Sentences with gene / chemical entities

Legend: GENE/PROTEIN CHEMICAL DISEASE SPECIES CELLLINE

Positive correlations were found between the level of carbonic anhydrase B and arterial CO2 tension and plasma HCO3 concentration . - pubmed
These findings suggest that the synthesis or degradation of carbonic anhydrase B isozyme is affected by arterial CO2 tension or plasma HC03 concentration . - pubmed
The 4 S to 5 S molar RNA ratio of the monosomes was calculated to be 0.6 , indicating 0.6 tRNA and/or aminoacyl tRNA molecule per ribosome ; no peptidyl tRNA was present . - pubmed
Diet , drug therapy ( Sulfonamide derivatives , biguanides , insulin ) ] . - pubmed
substance P ( measured as peak increases in lung resistance in cm H2O / ml per s ) at 6 h following antigen was noted in the presence of IL-1ra ( 30 mg / kg i.p . ) - pubmed
The behavioral effects of DMDS were reversed by naloxone and the kappa receptor antagonist nor - binaltorphimine diHCl , suggesting kappa opioid involvement in these behavioral changes . - pubmed
Below 14 degrees C , the data are consistent with a model in which interaction of MgATP with nitrogenase is fast and irreversible , and is followed by reversible electron transfer . - pubmed
From the extent and from the rate of the absorbance change , the rate constants for electron transfer from Fe protein to MoFe protein and of the reverse reaction were calculated . - pubmed
Analysis of the data obtained in the temperature range between 6 degrees C and 12 degrees C within the framework of the transition - state theory show that electron transfer from the Fe protein to the MoFe protein occurs via a highly disordered transition state with activation parameters delta H(0 ) + + = 289 kJ.mol-1 and delta S(0 ) + + = 792 J.K-1.mol-1 . - pubmed
Sequence segments of about 140 amino acids in length , each containing a selected consensus region , were used in alignments of the aminoacyl - tRNA synthetases with the aim of discerning their evolutionary relationships . - pubmed
Group II enzymes include those activating threonine , proline , serine , lysine , aspartic acid , asparagine , histidine , alanine , glycine , and phenylalanine . - pubmed
Within each group , enzymes specific for chemically similar amino acids tend to cluster together , indicating that a major theme of synthetase evolution involved the adaptation of binding sites to accommodate related amino acids with subsequent specialization to a single amino acid . - pubmed
In a few cases , however , synthetases activating dissimilar amino acids are grouped together . - pubmed
In order to increase water - solubility , solid complexes of polyacetylene compounds with alpha - cyclodextrin ( CD ) were prepared . - pubmed
Stimulation of platelets with thrombin leads to rapid degradation of inositol phospholipids , generation of diacylglycerol ( DAG ) and subsequent activation of protein kinase C ( PKC ) . - pubmed
Previous studies indicated that prior activation of PKC with phorbol myristate acetate ( PMA ) desensitizes platelets to thrombin stimulation , as indicated by a decreased production of inositol phosphates and decreased Ca2 + mobilization . - pubmed
This suggests that PKC activation generates negative - feedback signals , which limit the phosphoinositide response . - pubmed
To test this hypothesis further , we examined the effects of PKC activators and inhibitors on thrombin - stimulated DAG mass formation in platelets . - pubmed
Pretreatment with PMA abolishes thrombin - stimulated DAG formation ( 50 % inhibition at 60 nM ) . - pubmed
Pretreatment of platelets with the PKC inhibitors K252a or staurosporine potentiates DAG production in response to thrombin ( 3 - 4 - fold ) when using concentrations required to inhibit platelet PKC ( 1 - 10 microM ) . - pubmed
K252a does not inhibit phosphorylation of endogenous DAG or phosphorylation of a cell - permeant DAG in unstimulated platelets , indicating that DAG over - production is not due to inhibition of DAG kinase . - pubmed
Sphingosine , a PKC inhibitor with a different mechanism of action , also potentiates DAG formation in response to thrombin . - pubmed
Several lines of evidence indicate that DAG formation under the conditions employed occurs predominantly by phosphoinositide ( and not phosphatidylcholine ) hydrolysis : ( 1 ) PMA alone does not elicit DAG formation , but inhibits agonist - stimulated DAG formation ; ( 2 ) thrombin - stimulated DAG formation is inhibited by neomycin ( 1 - 10 mM ) but not by the phosphatidate phosphohydrolase inhibitor propranolol ; and ( 3 ) no metabolism of radiolabelled phosphatidylcholine was observed upon stimulation by thrombin or PMA . - pubmed
These data provide strong support for a role of PKC in limiting the extent of platelet phosphoinositide hydrolysis . - pubmed
In 6 a systemic accumulation of phytanic acid was demonstrated , together with low phytanic acid oxidase activity in skin fibroblasts in 5 of them . - pubmed
One dimensional SDS - PAGE showed four bands , present in control BLM and ischemic apical membranes , which could not be found in control apical membrane fractions . - pubmed
The effects of inositol hexaphosphate ( IHP ) and a second allosteric effector , bezafibrate , on the spin - state equilibria of the mixed - spin derivatives of ferric human hemoglobin A are examined . - pubmed
ACTH induced significant increases in plasma and salivary cortisol but in no case was a dose / response relationship detected . - pubmed
New information about the pharmacokinetics and pharmacodynamics of first - generation H1 receptor antagonists such as chlorpheniramine has become available in the past few years . - pubmed
Comprehensive pharmacokinetic and pharmacodynamic studies of new relatively nonsedating H1 receptor antagonists such as terfenadine , astemizole , loratadine , and cetirizine are appearing . - pubmed
Calpain II required 400 microM calcium , a reducing agent , and pH = 7.5 for maximal activity . - pubmed
These data suggest that RU 41740 acts directly on PMN membrane receptors . - pubmed
Serum testosterone and luteinizing hormone ( LH ) values remained within normal limits indicating resistance of Leydig cells to chemotherapy . - pubmed
Thus the drug has markedly altered the subcellular distribution of the glycoproteins so that they accumulate in the internal smooth membranes but are virtually absent in the plasma membrane . - pubmed
Significance of elevated urinary N - acetyl - beta - D - glucosaminidase activity in chronic cadmium poisoning . - pubmed
Urinary NAG activity increased with increasing beta 2 - m concentration up to about 100 U / g creatinine ; above this value the NAG activity did not increase with increasing beta 2 - m concentration . - pubmed
Urinary NAG activity thus represents another useful indicator of renal tubular damage caused by Cd poisoning since the mechanisms for the excretion of NAG and beta 2 - m into urine are thought to be different . - pubmed
It is concluded that PCMB releases the blocking effect of intracellular Na on apical Na channels . - pubmed
Bilirubin behaves as a peroxidase substrate for prostaglandin H synthase . - pubmed
Cell sublines of low and high metastatic potential expressed equally abl oncogene - coded mRNA and its phosphoprotein product p160 , and the capacity of p160 to become autophosphorylated with gamma -[ 32P ] ATP was the same among low and high metastatic cells . - pubmed
Regulation of corticosteroid hydroxylations : the intramitochondrial dielectric constant and proteolipids of adrenodoxin and cytochrome P450 . - pubmed
Applications : determination of plasma free fatty acids , serum lipase and post - heparin plasma triglyceride - lipases ] . - pubmed
The heme environment in horse heart ferrocytochrome c. - pubmed
Vascular smooth muscle differs from myocardial tissue in several critical ways : ( 1 ) calcium ion entry is achieved by at least two channels ; ( 2 ) both alpha-1 and alpha-2 postjunctional receptors regulate vascular tone ; ( 3 ) calmodulin increases the activity of the myosin light chain kinase ; ( 4 ) cyclic AMP decreases rather than increases cytosolic calcium ion concentration ; ( 5 ) angiotensin increases vascular tone without a positive inotropic effect by acting on angiotensin receptors which ultimately increase vascular cytosolic calcium . - pubmed
About 70 % of H-2Kb activity could be eluted from anti - H-2Kb - immunoadsorbents in the presence of 3 M NH4SCN and octyl glucoside ( pH 7.4 ) . - pubmed
On the mechanism of turnover of the carboxy - terminal tyrosine of the alpha chain of tubulin . - pubmed
The removal of tyrosine from the carboxyl terminus of the alpha chain of tubulin occurs predominantly from those tubulin dimers that are part of microtubules , and is dependent upon microtubular treadmill metabolism . - pubmed
This factor is neither tubulin : tyrosine ligase , the enzyme that catalyzes the addition of tyrosine to the carboxylterminal position of the alpha chain of tubulin , nor a carboxypeptidase - like activity . - pubmed
The reactions of metmyoglobin ( metMb ) and methemoglobin ( metHb ) , oxidized to their respective oxoferryl free radical species ( .Mb - FeIV = O/.Hb-4FeIV = O ) by tert - butyl hydroperoxide ( t - BuOOH ) , with nitric oxide ( NO . ) - pubmed
were studied by a combination of optical , electron spin resonance ( ESR ) , ionspray mass ( MS ) , fluorescence , and chemiluminescence spectrometries to gain insight into the mechanism by which NO . - pubmed
eliminated the signals of tyrosyl radical detected by ESR and oxoferryl detected by MS in the reaction of t - BuOOH with metMb . - pubmed
As evidenced by MS of apomyoglobin , this effect was due to the two - electron reduction of .Mb - FeIV = O by NO . - pubmed
The apparent volume of distribution was directly proportional to the nonalbumin protein concentration and ranged from 23 to 60 L. We conclude that the pharmacokinetics of 6 - MNA in this population are complicated by possible protein binding changes . - pubmed
We find that all transfectomas expressing IgM kappa encoded by VH 4 - 21 make IgM autoantibodies reactive with i , a linear poly - N - acetyllactosamine determinant present on neonatal red blood cells and a B cell - restricted isoform of the CD45 surface molecule . - pubmed
Thrombin induced a sustained contractile response with an initial transient increase in IP3 to about 160 % of the unstimulated control . - pubmed
Omission of extracellular Ca2 + or preincubation with verapamil ( 10 mumol / L ) reduced the maximum of contraction without significantly affecting the thrombin - induced increase in IP3 . - pubmed
To evaluate the role of PKC for the contractile response , the PKC was activated directly by phorbol 12,13 - dibutyrate ( PDBu , 50 nmol / L ) . - pubmed
Preincubation with the PKC inhibitor staurosporine ( 50 nmol / L ) significantly reduced the PDBu - induced contraction ( by about 80 % ) . - pubmed
In thrombin - stimulated vessels staurosporine only inhibited the tonic phase of the contractile response whereas the increase in IP3 and the phasic component of contraction were still evident . - pubmed
The phasic component of contraction is associated with the generation of IP3 ; the tonic component might be due to the activation of PKC . - pubmed
Microinjection of the excitatory amino acid L - glutamate into the nucleus tractus solitarii ( NTS ) elicits decreases in arterial pressure and heart rate . - pubmed
Microinjection of L - glutamate into the NTS caused rapid , transient , dose - related decreases in mean arterial pressure and heart rate . - pubmed
This study suggests that NTS neurons exert differential effects on renal , mesenteric , and hindquarter vascular beds and that glutamate - induced regional hemodynamic changes are mediated predominantly through autonomic pathways . - pubmed
Using altered DNA specificity Bicoid mutants and appropriate reporter genes , we show that Bicoid distinguishes among related DNA - binding sites in vivo by a specific contact between amino acid 9 of its recognition alpha - helix ( lysine 50 of the homeodomain ) and bp 7 of the site . - pubmed
Our results also demonstrate that Bicoid binds directly to those genes whose transcription it regulates and that the amino acid 9 contact is necessary for Bicoid to direct anterior pattern formation . - pubmed
In the beta - lactam resistant strains the enzyme activity and isoelectric points were determined on crude enzyme extract . - pubmed
The neurochemistry of vomiting involves serotonin and serotonin S3 receptors . - pubmed
The major classes of antiemetics include serotonin S3 receptor antagonists , phenothiazines and metoclopramide . - pubmed
We usually recommend a serotonin S3 receptor antagonist alone for less emetogenic regimens or in conjunction with dexamethasone for more emetogenic regimens . - pubmed
Subpicogram determination of oxytocin by an enzyme immunoassay using acetylcholinesterase as label . - pubmed
We measured the plasma ACTH and ME and PVN levels of CRH , and also of AVP , because AVP , another secretagogue of ACTH , has its cell body in the PVN and axon terminals partly in the ME . - pubmed
In the control perfusions , the hypothalamic release of CRH and AVP and the plasma ACTH did not change significantly during the entire period of observation. - pubmed
Ran / TC4 , in turn , interacts with a number of proteins that are involved in the regulation of GTP hydrolysis or are components of the nuclear pore . - pubmed
In solution binding assays , Ran - GTP bound p97 with high affinity , but the binding of Ran - GDP to p97 was undetectable . - pubmed
The addition of RanBP1 with Ran - GDP or Ran - GTP increased the affinity of both forms of Ran for p97 to the same level . - pubmed
Binding of Ran - GTP to p97 dissociated p97 from immobilized NLS receptor while the Ran - GDP / RanBP1 / p97 complex did not dissociate from the receptor . - pubmed
In a digitonin - permeabilized cell docking assay , RanBP1 stabilizes the receptor complex against temperature - dependent release from the pore . - pubmed
When added to an import assay with recombinant NLS receptor , p97 and Ran - GDP , RanBP1 significantly stimulates transport . - pubmed
These results suggest that RanBP1 promotes both the docking and translocation steps in nuclear protein import by stabilizing the interaction of Ran - GDP with p97 . - pubmed
To quantify the relatively large amount of O2.- generated over a 40 - min period by 1 x 10(6 ) granulocytes / mL , a discontinuous " 10 - min pulse " method employing cytochrome c was used ; 140 nmol O2.- per 1 x 10(6 ) cells was detected . - pubmed
The addition of catalase to remove extracellular H2O2 had no effect on DNA breakage . - pubmed
Renin - angiotensin system ( RAS ) is a methabolic pathway producing octapeptid angiotensin II ( AII ) with vasoconstrictive effects , which is also involved in the regulation of water homeostasis and electrolyte balance in the organism . - pubmed
The clock 's responses to light can , however , be reduced when glucose availability is decreased . - pubmed
Treatments were 1 ) control ( no added fat ) ; 2 ) tallow ( T ) , iodine value ( IV ) = 51.5 ; 3 ) partially hydrogenated tallow ( PHT ) , IV = 30.7 ; 4 ) hydrogenated tallow ( HT ) , IV = 6.9 ; 5 ) blend ( 1 : 1 ) of HT and hydrogenated free fatty acids ( HTHFA ) , IV = 9.0 ; and 6 ) hydrogenated free fatty acids ( HFA ) , IV = 11.2 . - pubmed
Flows of total C16 , total C18 , and total fatty acids to the duodenum were increased by supplemental fat ; flows of total C18 and total fatty acids were greater than their intake for all treatments . - pubmed
Digestibility of total fatty acids entering the small intestine ( 74 , 71 , 62 , 39 , 53 , and 63 % for treatments 1 to 6 , respectively ) was greater for the control diet than for fat - supplemented diets and decreased as either saturation ( T & lt ; PHT & lt ; HT ) or esterification ( HFA & lt ; HTHFA & lt ; HT ) increased . - pubmed
MAIN OUTCOME MEASUREMENTS : Muscle sympathetic nerve activity , assessed by microneurography of the peroneal nerve , blood pressure , heart rate , minute ventilation , transcutaneous oxygen saturation , and end tidal PCO(2 ) were measured during normal breathing , mask breathing , and CPAP at 5 and 10 cm H ( 2 ) O. - pubmed
Mono - chlorinated harmans were detected by concentration using blue rayon ( BR ) and GC / MS analysis in the final effluent from a sewage treatment plant in Shizuoka , Japan . - pubmed
Six separate alanine - substituted mutations were generated in 3 highly conserved motifs of FANCC . - pubmed
Cells bearing a naturally occurring FANCC mutation ( 322delG ) that preserves this conserved region showed normal STAT1 activation but remained hypersensitive to MMC . - pubmed
In addition , ( -)U50,488H stimulation enhanced phosphorylation of the Flag - hkor , but not Flag - rkor . - pubmed
( -)U50,488H - induced phosphorylation of the Flag - hkor was reduced by expression of the dominant negative mutant GRK2 - K220R , demonstrating the involvement of G protein - coupled receptor kinases ( GRKs ) . - pubmed
However , expression of GRK2 and arrestin-2 or GRK3 and arrestin-3 did not result in desensitization or phosphorylation of the Flag - rkor after ( -)U50,488H pretreatment . - pubmed
These results indicate that there are differences in ( -)U50,488H - induced desensitization and phosphorylation between the hkor and the rkor . - pubmed
Electron paramagnetic resonance ( EPR ) analysis showed two different signals , reflecting the presence of two different types of high - spin Fe sites with different symmetries . - pubmed
First , the W71V mutation led to a change in the relative proportion of the two Fe - based EPR signals . - pubmed
LPL plays a dual role in lipid metabolism both in the hydrolysis of triglyceride - rich lipoproteins and also as a molecular bridge , enhancing the receptor - mediated uptake of lipoproteins both by the liver ( anti - atherogenic ) but also by receptors on the artery wall ( pro - atherogenic ) . - pubmed
Results from a prospective study on CAD risk in healthy middle - aged men show that the risk associated with the LPL - D9N variant , which has a modest effect on plasma triglyceride levels , is enhanced up to 10 fold , but only in men who smoke . - pubmed
A BCR standard reference material of lichen ( CRM 482 ) was used to validate an analytical procedure consisting of a microwave oven digestion associated with ICP - OES , ICP - MS and GFAAS techniques for the analysis of trace elements ( Al , B , Ba , Cd , Co , Cr , Cu , Fe , Mn , Ni , Pb , Ti , V and Zn ) in biomonitor samples . - pubmed
All of the SECIS variants were shown to permit Sec insertion in phage ( by chemical modification of the Sec residue ) and fused to lacZalpha ( by beta - galactosidase assay ) . - pubmed
Exogenous expression of PEG10 conferred oncogenic activity and transfection of hepatoma cells with antisense S - oligonucleotides suppressing PEG10 resulted in their growth inhibition . - pubmed
OBJECTIVE : This study examined strength and bioactive ( remineralizing ) properties of a powder / liquid formulation ( Cement I ) and a more practical two - paste formulation ( Cement II ) of a fluoride - releasing resin - based Ca - PO ( 4 ) cement . - pubmed
On each tooth , two defined areas were coated , one with a composite resin ( calcium - free control ) , the other with either Cement I or II . - pubmed
Corticotropin and cortisone ; dosage and ratio of intramuscular and intravenous corticotropin ( ACTH ) and oral cortisone in treatment of certain dermatoses . - pubmed
Some characteristics of coagulation and von Willebrand factor during short term use of ticlopidine or clopidogrel ] . - pubmed
Levels of F1 + 2 , TAT and vWF were measured at baseline , on days 1 , 3 , 7 and 14 ( 7 days after discontinuation of thienopyridines ) . - pubmed
In substudy with clopidogrel differences between groups existed only in vWF levels . - pubmed
This could be interpreted as delayed effect of ticlopidine on thrombin activity . - pubmed
Functional expression of P2Y1 and P2Y2 / P2Y4 , but not P2Y6 , receptors in intact glomeruli was confirmed by measuring the relative stimulation of the inositol phosphate pathway induced by selective agonists of a particular receptor subtype . - pubmed
In the first assay stage , activated KSR1 is incubated with recombinant c - Raf-1 and ATP . - pubmed
In the second stage , activated c - Raf-1 is separated from KSR1 , and incubated with unactivated MEK1 , unactivated MAPK , Elk-1 , and ATP . - pubmed
Further , a complete inactivation of KSR1 - bound MEK1 by pretreating with the MEK inhibitor PD 98059 prior to the first assay stage did not alter KSR1 kinase activity . - pubmed
PURPOSE : Muscle insulin resistance is characterized by the inability of a normal insulin concentration to produce a favorable rate of glucose uptake . - pubmed
, glucose transport , is correlated with an increased expression of GLUT4 protein . - pubmed
Preventing GLUT4 overexpression during exercise training will inhibit the improvement in insulin - stimulated glucose transport . - pubmed
Urinary zinc excretion and serum insulin were significantly higher in the same group , although the insulinemia and the parameters of zinc nutritional status were not significantly correlated . - pubmed
The 5 - HT4 receptor agonist , tegaserod , is a potent 5 - HT2B receptor antagonist in vitro and in vivo . - pubmed
1 Tegaserod ( Zelnorm ) is a potent 5 - hydroxytryptamine4 ( 5 - HT4 ) receptor agonist with clinical efficacy in disorders associated with reduced gastrointestinal motility and transit . - pubmed
The present study investigated the interaction of tegaserod with 5 - HT2 receptors , and compared its potency in this respect to its 5 - HT4 receptor agonist activity . - pubmed
5 The data from this study indicate that tegaserod antagonizes 5 - HT2B receptors at concentrations similar to those that activate 5 - HT4 receptors . - pubmed
It remains to be determined whether this 5 - HT2B receptor antagonist activity of tegaserod contributes to its clinical profile . - pubmed
In the first step of protein synthesis , aminoacyl - tRNA synthetases react with amino acid and ATP to form an enzyme - bound adenylate that , in the next step , reacts with tRNA to form aminoacyl - tRNA . - pubmed
The reaction liberates pyrophosphate ( PP ( i ) ) which , in turn , can be hydrolyzed by pyrophosphatase to drive efficient aminoacylation . - pubmed
The bound adenylate does not react efficiently with the liberated PP(i ) that normally competes with tRNA to resynthesize ATP and free amino acid . - pubmed
Effects of histone deacetylase inhibitor FR901228 on the expression level of telomerase reverse transcriptase in oral cancer . - pubmed
In the present study , the epigenetic effects of the histone deacetylase ( HDAC ) inhibitor FR901228 on hTERT transcription in oral cancer cell lines were analyzed by RT - PCR . - pubmed
Moreover , cotreatment of protein synthesis inhibitor cycloheximide ( CHX ) resulted in the induction of hTERT transcription by FR901228 . - pubmed
This suggests that the induction of hTERT by FR901228 requires de novo protein synthesis to some extent and is more likely a direct than an indirect effect on epigenetic changes such as histone acetylation / deacetylation . - pubmed
We further examined the effect of FR901228 on c - myc protein , which is one of the main hTERT transcription activators . - pubmed
FR901228 repressed c - myc protein only in the absence of CHX , and depended on the enhancement of de novo protein synthesis . - pubmed
Our results indicate that c - myc protein is repressed indirectly by FR901228 but may not contribute to FR901228 - induced hTERT transcription . - pubmed
The present study showed that the HDAC inhibitor FR901228 induced the hTERT gene by a complex mechanism that involved transcription factors other than c - myc , in addition to inhibition of histone deacetylation . - pubmed
A ladder of relative proton affinities ( PA ) for a series of modified uridines ( e.g. - pubmed
The studied heterodimers are constituted of a modified nucleoside and either an amino acid or a nucleoside with known PA value . - pubmed
These results allowed our PA ladder of uridine and deoxyuridine analogs substituted at the C-5 position in the uracil ring to be extended . - pubmed
While these complexes are triply lithium - bridged dimers in the solid state , a monomer / dimer equilibrium is observed in solution by NMR spectroscopy and ESI FT - ICR MS . - pubmed
ESI FT - ICR mass spectrometry provides detailed insight into the mechanisms with which monomeric triscatecholate complexes as well as single catechol ligands exchange in the dimers . - pubmed
The 5alpha - reductase enzymes types 1 and 2 responsible for converting testosterone to the more potent androgen dihydrotestosterone are encoded by the SRD5A1 and SRD5A2 genes , respectively . - pubmed
We performed indirect genetic association studies of SRD5A1 and SRD5A2 and the dihydrotestosterone / testosterone ratio that reflects the activity of 5alpha - reductase in 57 males with type 2 diabetes . - pubmed
We found evidence of significant association between a single nucleotide polymorphism in SRD5A1 and the dihydrotestosterone / testosterone ratio ( median 0.10 , interquartile range 0.08 vs. median 0.06 , interquartile range 0.04 , P = 0.009 ) . - pubmed
These results suggest that functional genetic variants might exist in or around SRD5A1 that affect the activity of the 5alpha - reductase enzyme type 1 and influence androgen levels . - pubmed
The two families represent the cupredoxins and multicopper oxidases , both containing blue copper - binding domains . - pubmed
Biochemical analysis showed that lethal mutations of Asp425 , Arg463 , Arg511 and His515 in the kinase module abolished polynucleotide kinase activity in vitro . - pubmed
During PWA assessment no quantitative differences were observed in PAH between both groups nor after the angiography with oxygen administration . - pubmed
A left T12 - L1 transforaminal epidural steroid injection was performed using the " safe triangle " technique ; there was appropriate spread of dye that was visualized and recorded as well as a " washout " image . - pubmed
Heart rate ( HR ) , gas exchange and respiratory parameters were analyzed throughout the trial , and measured blood lactate concentration ( [ La(bl ) ] at rest and immediately after the exercise completion . - pubmed
Detached plant leaves were treated with CF for 6 , 18 and 30 h , and assayed for changes in hydrogen peroxide content , total ascorbate and glutathione pools and redox ratios ( reduced form to total pool ) , as well as for changes in activities of ascorbate peroxidase , glutathione reductase and glutathione - S - transferase . - pubmed
Sequence analysis of cDNA clones and analysis of putative translation products revealed that most encoded amino acid sequences shared the similar conserved motifs found in the cloned plant disease resistance genes RPS2 , MLA6 , L6 , RPM1 , and Xa1 . - pubmed
A valine ( Val ) to alanine ( Ala ) substitution at amino acid 16 , occurring in the mitochondrial targeting sequence of the MnSOD gene , has been associated with an increase in urolithiasis risk . - pubmed
The effects of the collimator - detector response , including the penetration and scatter components due to high - energy 123I photons , are modeled using precalculated tables of Monte Carlo simulated point - source response functions obtained from sources in air at various distances from the face of the collimator . - pubmed
Non - small cell lung cancer : whole - body MR examination for M - stage assessment -- utility for whole - body diffusion - weighted imaging compared with integrated FDG PET / CT . - pubmed
RESULTS : Area under ROC curve ( A(z ) ) values of whole - body MR imaging with DW imaging ( A(z ) = 0.87 , P = .04 ) and integrated FDG PET / CT ( A(z ) = 0.89 , P = .02 ) were significantly larger than that of whole - body DW imaging ( A(z ) = 0.79 ) . - pubmed
Specificity and accuracy of whole - body MR imaging with ( specificity , P = .02 ; accuracy , P & lt ; .01 ) and that without DW imaging ( specificity , P = .02 ; accuracy , P = .01 ) and integrated FDG PET / CT ( specificity , P & lt ; .01 ; accuracy , P & lt ; .01 ) were significantly higher than those of whole - body DW imaging . - pubmed
The formation of full - length mRNA products was facilitated by including in the reaction mixture 100 mM sodium acetate , high concentrations of ribonucleoside triphosphates , and proteinase K. - pubmed
We found that the PX domain of FAD49 not only has affinity for phosphoinositides , but also binds to its third SH3 domain . - pubmed
Cation complexing of crown ethers using fluorescence spectroscopy , part II . - pubmed
Application of the FGF-2 inhibitor polyanethole sulfonic acid ( PAS ) blocked the downregulation of osteoglycin and interfered with arteriogenesis . - pubmed
The thermodynamics of reduction and His ligand protonation have been determined for a range of loop - contraction variants of the electron transferring type 1 copper protein azurin ( AZ ) . - pubmed
For AZPC , in which the native C - terminal loop containing the Cys , His and Met ligands has been replaced with the shorter sequence from plastocyanin ( PC ) and AZAMI , in which the even shorter amicyanin ( AMI ) loop has been inserted , the thermodynamics of reduction match those of the protein whose loop has been introduced which are different to the values for AZ . - pubmed
The results for AZAMI and AZPC are primarily due to protein based enthalpic effects related to the interaction of the metal with permanent protein dipoles from the loop , and to the decreased loop length which favors His ligand protonation in the cuprous proteins . - pubmed
Diary - documented improvements in urgency , urge incontinence , frequency , nocturia and nocturnal voids were compared with pre - washout ( on tolterodine ) and post - washout ( on no drug ) diary entries . - pubmed
BACKGROUND AND OBJECTIVES : Dexmedetomidine is a highly selective alpha2 - adrenoceptor agonist used in anesthesia for its hypnoanalgesic and cardiovascular effects . - pubmed
A mechanism of action for such chemoprevention has been provided by the agonism of the peroxisome proliferator - activated receptor - gamma by 5 - ASA , which unifies its efficacy as an anti - inflammatory and chemopreventive agent . - pubmed
In the future , even more effective agents based on 5 - ASA are expected , based on more powerful agonism of peroxisome proliferator - activated receptor - gamma ; 5 - ASA preparations have become ' trendy ' again . - pubmed
Although HDL - mediated cholesterol transport to the liver is well studied , cholesterol efflux from hepatocytes back to HDL is less well understood . - pubmed
, HDL3 over HDL2 ; 2 ) NBD - cholesterol efflux was rapid ( detected in 1 - 2 min ) and resolved into fast [ half time ( t((1/2 ) ) ) = 2.4 min , 6 % of total ] and slow ( t((1/2 ) ) = 26.5 min , 94 % of total ) pools , consistent with protein- and vesicle - mediated cholesterol transfer , respectively ; 3 ) SCP-2 gene ablation increased efflux of NBD - cholesterol , as well as [ ( 3 ) H ] cholesterol , albeit less so due to competition by esterification of [ ( 3 ) H ] cholesterol , but not NBD - cholesterol ; and 4 ) SCP-2 gene ablation increased initial rate ( 2.3 - fold ) and size ( 9.7 - fold ) of rapid effluxing sterol , suggesting an increased contribution of molecular cholesterol transfer . - pubmed
Other membrane proteins in cholesterol efflux [ SRB1 and ATP - binding cassettes ( ABC ) A-1 , ABCG-1 , ABCG-5 , and ABCG-8 ] and several soluble / vesicle - associated proteins facilitating intracellular cholesterol trafficking ( StARDs , NPCs , ORPs ) were not upregulated . - pubmed
However , loss of SCP-2 elicited twofold upregulation of liver fatty acid - binding protein ( L - FABP ) , a protein with lower affinity for cholesterol but higher cytosolic concentration than SCP-2 . - pubmed
Ablation of SCP-2 and L - FABP decreased HDL - mediated NBD - cholesterol efflux . - pubmed
These results indicate that SCP-2 expression plays a significant role in HDL - mediated cholesterol efflux by regulating the size of rapid vs. slow cholesterol efflux pools and/or eliciting concomitant upregulation of L - FABP in cultured primary hepatocytes . - pubmed
Cangrelor is a reversible , potent , competitive inhibitor of the ADP P2Y ( 12 ) receptor that is administered intravenously and rapidly achieves near complete inhibition of ADP - induced platelet aggregation . - pubmed
These include behavior ; feeding rate changes ; respiration rate , oxygen consumption , and osmoregulation alterations ; nucleic acids , protein , and glycogen synthesis ; cholinesterase activity inhibition ; ATPase activity ; and oxidative stress responses . - pubmed
This article describes the morphological and chemical characterization of stimuli - responsive functionalized silicon surfaces provided in parallel by atomic force spectroscopy ( AFM ) and Fourier transform infrared spectroscopy ( FT - IR ) enhanced by the single - beam sample reference attenuated total reflection method ( SBSR - ATR ) . - pubmed
The stimuli - responsive behavior of the surfaces was obtained by grafting - to in melt carboxyl - terminated poly - N - isopropylacryl amides ( PNIPAAM ) with different degree of polymerization ( DP ) on epoxide - functionalized silicon substrates . - pubmed
These two NF - kappaB factors colocalized with Tax proteins in dotted cytoplasmic structures targeted by calreticulin , a multi - process calcium - buffering chaperone . - pubmed
The Pt ( II )-complex has a strong ability to quench the intrinsic fluorescence of HSA . - pubmed
The aim of the present study was to describe the effects of beta - blocker metoprolol monotherapy and combined with calcium channel inhibitor felodipin on digital microcirculation in primary Raynaud ' s syndrome . - pubmed
Action at a distance : amino acid substitutions that affect binding of the phosphorylated CheY response regulator and catalysis of dephosphorylation can be far from the CheZ phosphatase active site . - pubmed
Furthermore , amino acid substitutions distant from the active site can affect CheZ catalytic activity and CheYp binding , perhaps via the propagation of structural or dynamic perturbations through a helical bundle . - pubmed
RON expression was induced by the tumor promoter , phorbol 12 - myristate 13 - acetate ( PMA ) , in gastric adenocarcinoma AGS cells . - pubmed
The factors of metabolic syndrome ( BMI , SBP and DBP , and blood levels of glucose , cholesterol , and triglycerides ) , separately and combined , were analysed in quintiles and for transformed z - scores ( mean transformed to 0 and standard deviation to 1 ) . - pubmed
At week 48 , 83 % of the patients ( 61 % HBeAg - positive ; 92 % HBeAg - negative ) achieved a virological response and 82 % ( 78 % HBeAg - positive ; 83 % HBeAg - negative ) of those with elevated baseline alanine aminotransferase showed a biochemical response . - pubmed
Twenty - six per cent ( 14/54 ) of the HBeAg - positive patients lost HBeAg and 22 % ( 12/54 ) achieved seroconversion to anti - HBe . - pubmed
Examinations included visual acuity , slit - lamp , fundus , fundus fluorescein angiography ( FFA ) , large vascular ultrasound , digital subtraction angiography ( DSA ) , erythrocyte sedimentation rate ( ESR ) and reactive protein C ( CRP ) . - pubmed
Prostanoids are derived from arachidonic acid by the sequential actions of phospholipase A ( 2 ) , cyclooxygenase ( COX ) , and specific prostaglandin ( PG ) synthases . - pubmed
PGI ( 2 ) acts mainly on the prostacyclin ( IP ) receptor , but because of receptor homology , PGI ( 2 ) analogs such as iloprost may act on other prostanoid receptors with variable affinities . - pubmed
PGI ( 2 )/ IP interaction stimulates G protein - coupled increase in cAMP and protein kinase A , resulting in decreased [ Ca(2+)](i ) , and could also cause inhibition of Rho kinase , leading to vascular smooth muscle relaxation . - pubmed
In addition , PGI ( 2 ) intracrine signaling may target nuclear peroxisome proliferator - activated receptors and regulate gene transcription . - pubmed
These observations have raised interest in the use of COX inhibitors and PGI ( 2 ) analogs in the management of pregnancy - associated and neonatal vascular disorders . - pubmed
The amount of implanted ( 125 ) I seeds was 12 - 20 seeds / person , with a mean of 16.5 seeds / person , and the matching peripheral dosage ( MPD ) was 80~100Gy . - pubmed
Hyper - methylated miR-203 dysregulates ABL1 and contributes to the nickel - induced tumorigenesis . - pubmed
A calyculin A - sensitive Ser / Thr phosphatase activity downregulates TAp63alpha phosphorylation and ATM kinase mediates phosphorylation . - pubmed
Atorvastatin is eliminated by CYP3A4 which follows carrier - mediated uptake into hepatocytes by OATP1B1 , OATP1B3 , and OATP2B1 . - pubmed
Multiple clinical studies demonstrated that OATP inhibition by rifampin had a greater impact on atorvastatin systemic concentration than itraconazole - mediated CYP3A4 inhibition . - pubmed
If it is assumed that the blood and hepatocyte compartments are differentiated by the concentration gradient that is established by OATPs , and if the rate of uptake into the hepatocyte is rate - determining to the elimination of atorvastatin from the body , then it is hypothesized that blood concentrations may not necessarily reflect liver concentrations . - pubmed
A tetrakis ( dibenzylmethanido ) Eu ( III ) complex as a ultraviolet ( UV ) excited phosphor was synthesized , and incorporated with mesoporous silica as core - shell ( CS ) , outer - shell ( OS ) and intermediate - shell ( IS ) architectures , using a combination of the self - organization process and the Stober method . - pubmed
The CS silica nanoparticle casted UV - LED exhibited the best perfomence with strong intensity and excellent thermal stability . - pubmed
FokI polymorphism in the vitamin D receptor gene ( VDR ) and its association with lumbar spine pathologies in the Italian population : a case - control study . - pubmed
Alterations in vitamin D homeostasis , mainly involving its nuclear receptor ( VDR ) , could have a role in the pathophysiology of the spine . - pubmed
Regulative peroxidase activity of DNA - linked hemin by graphene oxide for fluorescence DNA sensing . - pubmed
The inhibition effect of graphene oxide toward the peroxidase activity of DNA - linked hemin was identified and conveniently utilized in the design of a homogenous fluorescence strategy for DNA sensing with high sensitivity . - pubmed
Specimens of tetragonal zirconia polycrystal ( TZP ) and aluminum toughened zirconia ( ATZ ) were modified with glass solder matrices in two configurations which mainly consisted of SiO2 , Al2O3 , K2O , and Na2O. - pubmed
Several degradation intermediates of 3,4 - DCA such as 1,2 - dichlorobenzene , 2 - chloro-1,4 - benzoquinone , 3,4 - dichlorophenyl isocyanate , 2 - chlorohydroquinone , 3,4 - dichloronitrobenzene , and 3,4 - dichlorophenol were identified by gas chromatography mass spectrometry ( GC - MS ) analysis . - pubmed
BLASTp analysis and phylogenetic relationship strongly suggested that the amino acid sequence was a member of HSP70 family . - pubmed
Multiple sequence alignment revealed that SmHSP70 and other known HSP70 were highly conserved , especially in the regions of HSP70 family signatures , the bipartite nuclear targeting sequence , ATP / GTP - binding site motif and ' EEVD ' motif . - pubmed
CONCLUSION : The use of preoperative oral 150 mg pregabalin 1 h before surgery or IV 0.5 mg ketamine with induction of anesthesia can reduce postoperative opioid consumption in breast cancer surgery without change in sedation or PONV and with a good safety profile . - pubmed
LSPR spectra of nanoparticulate gold films , including Au island films prepared by evaporation on glass and annealing as well as immobilized Au nanoparticle ( NP ) films , were measured in the transmission and reflection modes . - pubmed
PURPOSE : Navitoclax ( ABT-263 ) , a novel , oral Bcl-2 inhibitor , enhances the antitumor effects of chemotherapy in vitro by lowering the apoptotic threshold . - pubmed
The processed DBM can be used alone , together with nanostructured hydroxyapatite ( nanoHA ) , or dispersed in a physiological carrier or hydrogel . - pubmed
The authors observed that the presence of PNIPAAM reduced osteoblast adhesion , while the addition of nanoHA increased osteoblast adhesion , proliferation , interleukin-6 ( IL-6 ) production , and reduced lactate dehydrogenase ( LDH ) production . - pubmed
Increasing concentrations of PNIPAAM in combination with nanoHA further increased osteoblast proliferation , and decreased IL-6 and LDH production . - pubmed
Incorporation of PNIPAAM in DBM enhanced hMSCs proliferation and collagen type - I production . - pubmed
Furthermore , a combination of PNIPAAM and nanoHA further increased alkaline phosphatase and osteocalcin production in hMSCs , independently from the concentration of PNIPAAM . - pubmed
This study shows that combinations of DBM with nanoHA and PNIPAAM seem to offer a promising route to enhance cell activity and induce osteogenic differentiation . - pubmed
We further showed that abolishing the intramolecular interaction by a Leu-449 to Arg mutation or inducing ATR - dependent Chk1 phosphorylation by DNA damage disrupts the closed conformation , leading to an open and activated conformation of Chk1 . - pubmed
Bioaccumulation factor ( BAF ) values in earthworms were also higher for PFASs ( 4.06 + -2.23 ) than PBDEs ( 0.02 + -0.02 ) . - pubmed
We further analyzed the main biofilm components with NaIO4 , proteinase K , and DNase . - pubmed
Quantitative real - time RT - PCR revealed positive correlations between biofilm formation restrained by subinhibitory concentrations of tigecycline and the expression of biofilm potential - associated genes , especially the AdeFGH efflux pump gene . - pubmed
Chemical cross - linking and coimmunoprecipitation experiments revealed that OATP1B1 may form homo - oligomers , possibly through disulfide bonds . - pubmed
When wild - type OATP1B1 was coexpressed with a loss - of - function mutant W258A , cells showed reduced uptake of prototypic substrate estrone-3 - sulfate ( ES ) . - pubmed
Light increases the ion and non - electrolyte permeability of rhodopsin - phospholipid vesicles . - pubmed
A rapid method for the purification of supercoiled PM2 DNA by affinity chromatography on H1 histone covalently coupled to agarose . - pubmed
A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of H1 histone covalently coupled to agarose . - pubmed
Increase in sialic acids removable by neuraminidase during the shape change of platelets . - pubmed
The shape change associated with activation gave rise to an increase in sialic acids removable by neuraminidase . - pubmed
[ Determination of mercury in surface , spring , and ground waters ] . - pubmed
Screening procedures are included for the following categories : barbiturates and other sedative - hypnotics , anticonvulsants , benzodiazepines , antidepressants , phenothiazine and butyrophenone neuroleptics , central stimulants ( amphetamines , cocaine ) , hallucinogens ( LSD , phencyclidine , tetrahydrocannabinol ) , opioid ( narcotic ) and other potent analgesics , non - opioid analgesics , antihistamines ( histamine H1 - receptor blockers ) , antiparkinsonian drugs , beta - blockers ( beta - adrenoceptor blockers ) , antiarrhythmics ( class I and IV ) , diuretics , laxatives and their metabolites . - pubmed
Treatment with DHT caused a dose - dependent inhibition of testicular 3 beta - hydroxysteroid dehydrogenase / delta 5 - 4 isomerase ( 3 beta - HSD ) 2 h later , and this effect was apparent at the dose of 20 micrograms/100 g body weight ( P less than 0.01 ) . - pubmed
The inhibitory effect of 3 beta - HSD was not due to a possible interference of DHT in the enzyme assay , since various concentrations of the androgen ( 0.1 - 100 mumol / l ) were ineffective as inhibitors of 3 beta - HSD . - pubmed
The highest dose of DHT used in this study ( 200 micrograms/100 g body weight ) resulted in a rapid ( 1 - 2 h ) and transient ( 4 - 6 h ) inhibition ( approximately 80 % ) of 3 beta - HSD activity . - pubmed
The results presented suggest that the inhibitory effect of the non - aromatizable androgen DHT is receptor - mediated and involves the synthesis of a factor(s ) that modulates 3 beta - HSD activity . - pubmed
Perfusion with both acetylcholine and epinephrine ( 10(-5 ) to 10(-3 ) M ) produced concentration - dependent increases in mean CBF ( maximum increases at 10(-3 ) M of 16 and 9 % , P less than 0.05 ) , whereas only acetylcholine increased mean SLV ( +56 % at 10(-3 ) M , P less than 0.05 ) . - pubmed
The influence of calcium , sodium , and the Na+/Ca2 + antiport on susceptibility to cytolysin / perforin - mediated cytolysis . - pubmed
In NK cell - mediated cytolysis , the degranulation of the NK cell , and the binding and polymerization of its effector molecule cytolysin / perforin , are Ca2 + dependent . - pubmed
To determine if increases in the target cell free intracellular Ca2 + concentration ( [ Ca2+]i ) are also important for cytotoxicity , we examined the effects of Ca2 + substitutes , promotors of Ca2 + influx ; and inhibitors of Ca2 + efflux on the cytolysis mediated by purified cytolysin . - pubmed
Ca2 + promoted cytolysin - mediated cytolysis but could also inactivate cytolysin on preincubation in the absence of target cells . - pubmed
Ba2 + and Sr2 + could not alone promote cytolysin - mediated cytolysis but did enhance Ca2 (+)-driven cytolysis . - pubmed
Preincubation with Ba2 + and Sr2 + did not inactivate cytolysin . - pubmed
One interpretation which these results suggested was that neither Ba2 + nor Sr2 + were involved in cytolysin polymerization and pore formation , but could act intracellularly as Ca2 + analogs once pore formation had occurred . - pubmed
The synergistic interaction of cytolysin and the Ca2 + ionophore A23187 further supported a role for increased [ Ca2 +]i in cytolysis . - pubmed
Inhibitors of Na+/Ca2 + exchange , namely low Na+ medium , ouabain , and the amiloride analogs 2 ' , 4 '- dimethylbenzamil , 5 -( N-4 - chlorobenzyl )- 2 ' , 4'dimethylbenzamil , and alpha - phenylbenzamil all markedly enhanced cytolysin - mediated cytolysis . - pubmed
This open reading frame contains amino acid sequences identical to 13 N - terminal amino acids of purified factor Y , as well as to a 10 - amino acid internal sequence ( from a cyanogen bromide fragment ) as determined by gas - phase microsequencing . - pubmed
Naloxone caused a significant decrease and captorile -- increase of maximum meanings of HR during exercises ( the running on the treadmill during 3.5 min by the velocity of 30 m / min ) , both compounds did n't exert a considerable effect on BP at rest and during exercises . - pubmed
An inhibitory effect of IL 1 on insulin secretion is not observed when islets are coincubated with an inhibitor of DNA transcription ( actinomycin D ) . - pubmed
The inhibitory effect of IL 1 on insulin secretion also requires mRNA translation , because three structurally distinct inhibitors of protein synthesis ( cycloheximide , anisomycin , and puromycin ) prevent IL 1 - induced inhibition of insulin secretion when added to islets after the 1 - h exposure to IL 1 . - pubmed
Two - dimensional gel electrophoresis of islet proteins metabolically labeled with [ 35S ] methionine demonstrates that IL 1 augments the expression of a 65 - kD ( pl approximately 6.5 ) protein by greater than 2.5 - fold . - pubmed
Of all isoenzymes tested the human isoenzyme mu is most sensitive to the action of both ethacrynic acid and its glutathione conjugate . - pubmed
Furthermore , the content of the ANT mRNA in adult islets cultured at a high glucose concentration was increased compared with islets cultured at a low glucose concentration . - pubmed
Finally , streptozocin - treated islets , which display an impaired glucose - sensitive insulin release after 6 days in culture , also contained less of this mRNA than the control islets . - pubmed
Beta 1 - selective adrenoceptor antagonists : examples of the 2 -[ 4 -[ 3 -( substituted amino )- 2 - hydroxypropoxy ] phenyl ] imidazole class . - pubmed
An attempt to develop a highly cardioselective beta - adrenoceptor antagonist devoid of intrinsic sympathomimetic activity ( ISA ) focused on exploring structure - activity relationships around ( S )-[ p -[ 3 -[[ 2 -( 3,4 - dimethoxyphenyl ) ethyl ] amino ]- 2 - hydroxypropoxy ] phenyl ]- 4 -( 2 - thienyl ) imidazole . - pubmed
( S )- 4 - Acetyl-2 -[[ 4 -[ 3 -[[ 2 -( 3 , 4 - dimethoxyphenyl ) ethyl ] amino ]- 2 - hydroxypropoxy ] phenyl ] methyl ] imidazole ( S-51 ) was demonstrated to be highly cardioselective ( dose ratio beta 2 / beta 1 greater than 9333 ) and devoid of ISA . - pubmed
This indicates that adequate supply and balance of amino acids are required both for the increase in polysome - associated ODC - mRNA activity and for the translational and/or posttranslational mechanism(s ) during the induction of hepatic ODC activity . - pubmed
Interaction of ligands in phosphorylase A as monitored by crosslinking and enzymatic modifications : synergism of glucose and caffeine manifested in the exposure of N - terminal segment . - pubmed
Glycogen , caffeine and glucose dissociate phosphorylase a tetramer to dimers with half - maximum effect at 0.16 % , 1.1 and 71 mM concentration , respectively , as monitored by crosslinking with dimethyl suberimidate at 18 degrees C. 2 . - pubmed
These results indicate that the vasorelaxant effect of butein depended on the endothelium and was mediated by endothelium - derived relaxing factor ( EDRF ) . - pubmed
cAMP - specific phosphodiesterase ( type IV ) activity was potently inhibited by butein with an IC50 of 10.4 + /- 0.4 microM. - pubmed
In contrast , phosphodiesterase I , III and V activities were inhibited by butein above 100 microM. - pubmed
Adenylate cyclase and guanylate cyclase activities were unchanged by butein . - pubmed
These results suggest that the increase of cAMP formation elicited by butein is due to the inhibition of cAMP - specific phosphodiesterase . - pubmed
PDI also enhanced the yield of PelC in DsbA- cells but only in the presence of exogenous oxidized glutathione . - pubmed
Taurocholate feeding decreased ileal reductase activity 48 % , whereas tauroursocholate , the 7 beta - hydroxy epimer of taurocholate , had no effect . - pubmed
Feeding lovastatin ( inhibitor of HMG - CoA reductase ) stimulated total ileal HMG - CoA reductase activity threefold in washed microsomes , which were dissociated from the inhibitor . - pubmed
The inhibition of intestinal HMG - CoA reductase activity by taurocholate occurred without accumulation of mucosal cholesterol . - pubmed
Furthermore , the stimulation of total ileal HMG - CoA reductase activity by lovastatin treatment was observed without a decrease in mucosal cholesterol . - pubmed
In summary , the regulation of ileal HMG - CoA reductase activity by the intestinal luminal flux of bile acids is dependent on the orientation of the hydroxyl groups. - pubmed
Mutations in the fur ( ferric uptake regulator ) locus that produce an Atr - acid - sensitive phenotype also eliminate induction of six transiently induced ASPs . - pubmed
so that basal and LH - stimulated ovarian steroid secretion could be determined . - pubmed
The dopamine transporter ( DAT ) in pre - synaptic membranes and the vesicular monoamine transporter 2 ( VMAT2 ) in membranes of synaptic vesicles are involved in mediating the acute effects of amphetamine on dopamine transmission . - pubmed
Thus , repeated amphetamine administration exerts modest and regionally selective effects on DAT and VMAT2 mRNA expression in subpopulations of midbrain dopamine neurons . - pubmed
Instead , we found that the GST - Cdc6 can compete with distamycin A for binding to the DNA molecule . - pubmed
As distamycin A is a specific reagent that binds noncovalently to DNA at ( A + T)-rich tracks , the stimulation of Abf1 DNA binding activity may be mediated by the Cdc6 / DNA interaction . - pubmed
The method involved precipitation of plasma protein with methanol followed by analysis of the supernatant using reversed - phase column and UV detection at 316 nm . - pubmed
The amino acid transport system y+L / 4F2hc is a heteromultimeric complex . - pubmed
We show that expression of system y+L - like amino acid transport activity by 4F2hc in oocytes is limited by an endogenous factor and that direct covalent modification of external cysteine residue(s ) of an oocyte membrane protein blocks system y+L/4F2hc transport activity , based on the following . - pubmed
Mutation CS2 had no effect on the expressed system y+L - like transport activity , whereas C109S - containing mutants ( CS1 and Cys - less ) retained only partial y+L - like transport activity ( 30 to 50 % of wild type ) . - pubmed
4 ) Sensitivity to Hg2 + inactivation is increased by pretreatment of oocytes with ss - mercaptoethanol or in the C109S - containing mutants ( CS1 and Cys - less ) . - pubmed
These results indicate that 4F2hc is intimately associated with a membrane oocyte protein for the expression of system y+L amino acid transport activity . - pubmed
The ribozyme inhibited the formation of P - glycoprotein and reduced the cell 's resistance to adriamycin . - pubmed
Three RIIalpha isoforms of 51 , 54 , and 57 kDa were identified by RIIalpha immunodetection and labeling with 8 - azido [ 32P ] cAMP in different cell cycle phases . - pubmed
The membrane - bound quinohaemoprotein alcohol dehydrogenases pass electrons to membrane ubiquinone which is then oxidized directly by ubiquinol oxidases . - pubmed
The electron acceptor for membrane glucose dehydrogenase is ubiquinone which is subsequently oxidized directly by ubiquinol oxidases or by electron transfer chains involving cytochrome bc1 , cytochrome c and cytochrome c oxidases . - pubmed
Synthesis of the quinoprotein respiratory systems requires production of PQQ , haem and the dehydrogenase subunits , transport of these into the periplasm , and incorporation together with divalent cations , into active quinoproteins and quinohaemoproteins . - pubmed
Analgesic profile of the nicotinic acetylcholine receptor agonists , ( + ) - epibatidine and ABT-594 in models of persistent inflammatory and neuropathic pain . - pubmed
Allosteric interactions of staurosporine and other indolocarbazoles with N -[ methyl -( 3 ) H ] scopolamine and acetylcholine at muscarinic receptor subtypes : identification of a second allosteric site . - pubmed
We have studied the interactions of five indolocarbazoles with N -[ methyl -( 3 ) H ] scopolamine ( NMS ) and unlabeled acetylcholine at M ( 1 )- M ( 4 ) muscarinic receptors , using equilibrium and nonequilibrium radioligand binding studies . - pubmed
[ ( 3 ) H ] NMS binding was enhanced by staurosporine , KT5720 , and KT5823 at M ( 1 ) and M ( 2 ) receptors , and by K-252a at M ( 1 ) receptors . - pubmed
A range of cooperative effects with acetylcholine was seen , and , at the M ( 1 ) receptor , KT5720 had a log affinity of 6.4 and enhanced acetylcholine affinity by 40 % . - pubmed
The compounds inhibited the dissociation of [ ( 3 ) H ] NMS to different extents across the receptor subtypes , with the largest effects at M ( 1 ) receptors . - pubmed
In equilibrium binding studies the inhibitory potency of gallamine at M ( 1 ) receptors was not affected by KT5720 , indicating that these agents bind to two distinct allosteric sites and have neutral cooperativity with each other . - pubmed
In contrast , gallamine and staurosporine had a negatively cooperative or competitive interaction at M ( 1 ) receptors . - pubmed
Similarly , the potency and relative effectiveness of KT5720 for inhibiting [ ( 3 ) H ] NMS dissociation from M ( 1 ) receptors were not affected by gallamine or brucine , but were affected in a complex manner by staurosporine . - pubmed
These results demonstrate that there are at least two distinct allosteric sites on the M ( 1 ) receptor , both of which can support positive cooperativity with acetylcholine . - pubmed
Previous research indicated that allantoic acid degradation is catalysed by different enzymes in cultivars Maple Arrow and Williams . - pubmed
The enzyme found in Williams requires manganese as a cofactor . - pubmed
The solubilization of proteinases with 0.5 % Triton X-100 substantially improved the yield of the enzymes . - pubmed
The high trans alpha - linolenic acid diet significantly increased the plasma LDL-:HDL - cholesterol ratio by 8.1 % ( 95 % CI 1.4 , 15.3 ; and the total cholesterol : HDL - cholesterol ratio by 5.1 % ( 95 % CI 0.4 , 9.9 ; compared with the low - trans diet . - pubmed
This was largely explained by an increase in LDL - cholesterol on the high - trans diet , while no change was observed in the low - trans group ( mean treatment effect of 4.7 % ( 95 % CI -0.8 , 10.5 ; No effects were found on total cholesterol and HDL - cholesterol , triacylglycerols , apolipoprotein B and A-1 , and lipoprotein ( a ) concentrations . - pubmed
In conclusion , trans alpha - linolenic acid may increase plasma LDL-:HDL - cholesterol and total cholesterol : HDL - cholesterol ratios . - pubmed
Platelet aggregation in response to ADP , collagen , and PGE1 was assessed , using optical aggregometry . - pubmed
Addition of PGE1 prior to addition of an agonist inhibited platelet aggregation in a concentration - dependent manner , whereas addition of PGE1 4 minutes after addition of ADP resulted in partial reversal of aggregation and fibrinogen binding . - pubmed
CONCLUSIONS AND CLINICAL RELEVANCE : A high concentration of sodium citrate in blood samples decreases plasma concentration of ionized calcium , resulting in reduced platelet aggregation and fibrinogen binding . - pubmed
Platelet aggregation and fibrinogen binding can be reversed by PGE1 , which may result in underestimation of platelet activation . - pubmed
The analytes were extracted with methanol followed by isocratic chromatography on a micro C18 reversed - phase column using acetonitrile - water , 50:50 ( v / v ) , containing 20 mm acetic acid at 30 microl min(-1 ) . - pubmed
Polysaccharide recognition by surfactant protein D : novel interactions of a C - type lectin with nonterminal glucosyl residues . - pubmed
As with the mannose - binding proteins , we found significant hydrogen bonding between the protein and the vicinal , equatorial OH groups at the 3 and 4 positions on the sugar ring . - pubmed
By using various soluble glucosyl homopolysaccharides as inhibitors of SP - D carbohydrate binding , we confirmed the interactions predicted by our modeling studies . - pubmed
Diagnosis and dosimetry of exposure to sulfur mustard : development of a standard operating procedure for mass spectrometric analysis of haemoglobin adducts : exploratory research on albumin and keratin adducts . - pubmed
Experiments were carried out to develop a standard operating procedure for analysis of sulfur mustard adducts to the N - terminal valine in haemoglobin and to explore adduct formation with albumin and keratin . - pubmed
Influx of this model permeant by these cells exhibited characteristics of carrier - mediated membrane transport and was only minimally reduced by the addition of 5 micromol / L folic acid , a concentration of this natural folate that would have completely inhibited influx by the folate receptor . - pubmed
Gene - expression analysis revealed prominent expression of the folate receptor in some but not all mesothelioma cell lines , and in only one case was expression of this receptor gene greater than expression of the reduced folate carrier gene . - pubmed
CONCLUSION : In view of the ongoing reduced folate carrier - mediated internalization that occurs in mesothelioma cells , these results would seem to argue against a role for the folate receptor in the internalization and cellular pharmacokinetics of methotrexate and other classic folate analogs in this neoplasm . - pubmed
Furthermore , chronic ethanol consumption may damage the liver by inhibiting the hepatotrophic and hepatoprotective actions of TNF alpha and other cytokines . - pubmed
It suggested that liver - associated T lymphocytes are involved in the inflammatory process associated with alcohol related liver injury through increased cytokine secretion ( TNF alpha ) . - pubmed
Parallel solution- and solid - phase synthesis of spirohydantoin derivatives as neurokinin-1 receptor ligands . - pubmed
The combination of the 3,5 - bis ( trifluoromethyl ) phenyl group with a spirohydantoin motive as a central scaffold was the basis for the design of a combinatorial library targeted towards the neurokinin-1 receptor . - pubmed
strain CT16 , purification , and characterization of a deoxycytidine deaminase extremely thermostable in the presence of D , L - dithiothreitol . - pubmed
A deoxycytidine deaminase that was extremely thermostable in the presence of dithiothreitol was found in a mesophilic bacterium isolated from soil . - pubmed
The enzyme was purified to a homogeneous protein by treatment at 100 degrees C , ammonium sulfate precipitation , and chromatography on DEAE - Toyopearl , hydroxyapatite , and then Sephacryl S-100 . - pubmed
After treatment at 50 degrees C for 15 min in the absence of dithiothreitol , enzyme activity was 44 % of the starting activity ; after treatment at 100 degrees C for 2 h in the presence of 50 mm dithiothreitol , activity was 56 % of the starting activity . - pubmed
Dithiothreitol greatly stabilized the enzyme . - pubmed
The enzyme molecule was dissociated into two subunits each of 18 kDa subunit when reduced with mercaptoethanol . - pubmed
Lipid peroxidation may be initiated either by lipoxygenases or by reactive oxygen species ( ROS ) . - pubmed
PPA1 and PPB1 display powerful biologic activities including activation of mitogen - activated protein kinase ( MAPK ) and induction of glutathione - S - transferase ( GST ) , defense genes , and phytoalexins . - pubmed
However , a lack of change in serum cortisol concentration and body mass and dynamics in immunoglobulin protein , serum protein , and testes mass were not consistent with previous reports of semelparous dasyurid physiology . - pubmed
Further experiments indicated that Ms or Mt RecA proteins catalyzed adenosine triphosphate hydrolysis at approximately comparable rates across a wide range of pHs . - pubmed
These changes were most probably due to specific interactions of the salt ions with the charged amino acids on the protein surface rather than due to general effects such as Hofmeister and Debye - Huckel . - pubmed
We also found significant inverse correlations between OPGc ( corrected for BMI ) and fasting glucose ( r = -0.325 , p = 0.005 ) , fasting insulin ( r = -0.404 , p = 0.0001 ) as well as HOMA - IR ( r = -0.428 , p = 0.0001 ) . - pubmed
Cerebral MRI revealed diffuse hyperintense T2 abnormalities in the supratentorial white matter without cerebellar atrophy or lesions , while Technetium-99m - ECD brain SPECT revealed a severe cerebellar hypoperfusion . - pubmed
Agonists for aggregation were adenosine diphosphate , arachidonic acid , collagen , epinephrine , and two concentrations of ristocetin . - pubmed
Flow cytometry analysis using fluorescence - activated cell sorting with fluorescein isothiocyanate - labeled annexin V demonstrated that the TTSS1 - dependent cell death was by apoptosis . - pubmed
AIM : To investigate the distensibility of the common carotid artery ( CCA ) , baroreflex sensitivity ( BRS ) and its relation to plasma homocysteine concentration in exfoliation syndrome or exfoliation glaucoma ( XFS / XFG ) . - pubmed
In women independent associations with ASB were found for urinary incontinence ( OR : 2.99 , CI : 1.60 - 5.60 ) , reduced mobility ( OR : 2.68 , CI : 1.42 - 5.03 ) and oestrogen treatment ( OR : 2.20 , CI : 1.09 - 4.45 ) . - pubmed
RASGRF2 expression was restored after treatment with the demethylating agent , 5 - aza-2 '- deoxycytidine in all four cell lines tested that downregulated RASGRF2 expression . - pubmed
Hen egg white lysozyme ( HEWL ) and a synthetic model peptide defining HEWL helix-4 were used as comparative model systems to systematically investigate the effect of increasing TFE concentrations on the structure of proteins and peptides . - pubmed
Higher TFE concentrations denatured HEWL with the loss of lysozyme tertiary structure . - pubmed
Low Serum Selenium Is Associated with Anemia Among Older Women Living in the Community : the Women 's Health and Aging Studies I and II . - pubmed
An increase in loge selenium was associated with a reduced risk of anemia ( odds ratio per 1 SD increase = 0.63 , 95 % confidence interval = 0.47 - 0.84 ) , adjusting for age , education , chronic diseases , iron status , and serum interleukin-6 . - pubmed
Pattern of nucleotide substitution and divergence of prophenoloxidase in decapods . - pubmed
Despite the unprecedented development in identification and characterization of prophenoloxidase ( proPO ) in commercially important decapods , little is known about the evolutionary relationship , rate of amino acid replacement and differential selection pressures operating on proPO of different species of decapods . - pubmed
Previously , we showed that tyrosine phosphorylation of p130Cas ( Cas ) in a cytoskeletal complex is involved in force - dependent activation of the small GTPase Rap1 . - pubmed
In univariate analysis , leukocytes and CRP levels were associated with MVO , whereas pre - infarction angina and prior medication by aspirin or calcium channel antagonist appeared protective . - pubmed
Alteplase ( TPA ) for clotted dialysis catheters . - pubmed
The patient had received intravenous dextrose infusions while undergoing a surgical procedure for post - traumatic hydrocele elsewhere a week prior to this episode . - pubmed
Average combined turnover numbers of 35,800 + /- 20,900 ( N = 302 ) and 31,700 + /- 17,700 min(-1 ) ( N = 315 ) were obtained respectively for the in vitro synthesized enzyme from strain 35321 with the absence and presence of a C - terminal His(6 ) tag . - pubmed
The average combined turnover numbers of the enzyme from both strains synthesized in vivo and in vitro and with the presence and absence of a His(6 ) tag were found to differ significantly . - pubmed
This indicates that the in vivo and in vitro produced enzymes are not identical and the presence of a C - terminal His(6 ) tag alters the activity of beta - galactosidase . - pubmed
Separation of the amplification products on 4.5 % agarose gels allowed the comparative assessment of the classical type I IFNs . - pubmed
During follow - up ( postoperative ) , a decrease occurred in the body mass index ( mean + /- standard deviation 49.2 + /- 8.7 kg / m(2 ) to 34.1 + /- 8.1 kg / m(2 ) ; P & lt;.0001 ) , excess body weight ( mean + /- SD 76.1 + /- 23.6 kg to 34.9 + /- 21.7 kg ; P & lt;.0001 ) , hemoglobin A1c ( mean + /- SD 6.5 % + /- 1.3 % to 5.6 % + /- 0.8 % ; P & lt;.0001 ) , systolic blood pressure ( mean + /- SD 133.7 + /- 14.3 mm Hg to 112.9 + /- 14.6 mm Hg ; P & lt ; .0001 ) , urine albumin creatinine ratio ( from a median of 8.0 mg / g [ interquartile range 5.0 - 29.3 ] to a median of 6.0 mg / g [ interquartile range 3.3 - 11.5 ] ; P & lt;.0001 ) , and hs - CRP ( mean + /- SD 11.2 + /- 9.8 mg / L to 4.7 + /- 5.9 mg / L ; P & lt;.0001 ) . - pubmed
This study was designed to investigate the effects of activation of cholecystokinin CCK ( 1 ) and serotonin ( 5 - hydroxytryptamine , 5 - HT ) 5 - HT ( 3 ) receptors on pancreatic vagal afferent discharge and to determine whether there is an interaction between these receptors . - pubmed
The effects of systemic administration of cholecystokinin and the serotonin 5 - HT ( 3 ) receptor agonist phenylbiguanide on pancreatic vagal afferent discharge were recorded before and after administration of cholecystokinin CCK ( 1 ) and serotonin 5 - HT ( 3 ) receptor antagonists . - pubmed
Cholecystokinin CCK ( 1 ) receptor antagonists , lorglumide ( 10 mg / kg , i.v . ) - pubmed
On the other hand , serotonin 5 - HT ( 3 ) receptor blockade with granisetron ( 1 mg / kg , i.v . ) - pubmed
This study has confirmed that cholecystokinin and phenylbiguanide activate pancreatic vagal afferent discharge via activation of cholecystokinin CCK ( 1 ) and serotonin 5 - HT ( 3 ) receptors , respectively . - pubmed
In addition , it has demonstrated that ( i ) the serotonin 5 - HT(3 ) agonist phenylbiguanide acts partly via an interaction with cholecystokinin CCK ( 1 ) receptors , and ( ii ) the actions of cholecystokinin are not dependent on serotonin 5 - HT ( 3 ) receptor activation . - pubmed
Synthesis and antibacterial property of quinolines with potent DNA gyrase activity . - pubmed
BACKGROUND : Studies have suggested that vitamin D may be important for both insulin sensitivity and insulin secretion , and that supplementation with vitamin D may subsequently prevent development of type 2 diabetes . - pubmed
The insulin and metformin doses were not changed throughout the study . - pubmed
RESULTS : After 6 months , the fasting glucose , insulin , C - peptide , fructosamine , and HbA ( 1c ) levels were not significantly different from baseline values . - pubmed
miR-22 overexpression leads to a reduction of ER alpha levels , at least in part by inducing mRNA degradation , and compromises estrogen signaling , as exemplified by its inhibitory impact on the ER alpha - dependent proliferation of breast cancer cells . - pubmed
Role of FOXA in mitochondrial citrate carrier gene expression and insulin secretion . - pubmed
In this study , we have investigated the transcriptional role of the FOXA site present in the promoter of the mitochondrial citrate carrier ( CIC ) gene . - pubmed
podocalyxin like-1 , tribbles 3 , reactive oxygen species , and nuclear factor kappa B ) ? - pubmed
Its metal - independent nature , molecular weight of 14.5 kDa , preferential affinity for beta - D - lactose , and 87 - 92 % identity with carbohydrate recognition domain of previously reported galectin-1 confirmed its inclusion in galectin-1 subfamily . - pubmed
Treatment of the purified lectin with oxidizing agent , thiol blocking reagents , denaturants , and detergents resulted in significant changes in UV - VIS , fluorescence , CD and FTIR spectra , which strongly emphasized the important aspect of regular secondary structure of galectins for the maintenance of their active conformation . - pubmed
Reduction of the activity of the purified lectin after oxidation by H2O2 , with remarkable fluorescence quenching , may suggest potential role for galectin-1 in free radical - induced , oxidative stress - mediated cardiovascular disorders . - pubmed
PURPOSE : To test interobserver agreement in the fluorescein / indocyanine green angiography ( FA / ICGA ) scoring system for uveitis . - pubmed
These surrogate factors were fasting insulin , hemoglobin A1c ( HbA1c ) , high - sensitivity C - reactive protein ( hsCRP ) , and uric acid . - pubmed
METHODS : Waist circumference ( WC ) , blood pressure , triglycerides , high - density lipoprotein cholesterol ( HDL - C ) , fasting glucose , fasting insulin , HbA1c , hsCRP , and uric acid measurements were obtained from 2,624 adolescent ( 12 - 18 years old ) participants of the 1999 - 2006 National Health and Nutrition Examination Surveys . - pubmed
Metabolic syndrome criteria had the highest sensitivity for predicting fasting insulin ( 40 - 70 % ) , followed by uric acid ( 31 - 54 % ) , hsCRP ( 13 - 31 % ) , and HbA1c ( 7 - 21 % ) . - pubmed
Despite Wzx homologs performing a conserved function , it has been difficult to pinpoint specific motifs of functional significance in their amino acid sequences . - pubmed
Four TM helices ( II , IV , X , and XI ) contain polar residues ( aspartic acid or lysine ) , and they may form part of a relatively hydrophilic core . - pubmed
Aspartic acid-85 and lysine-419 are located in TM helices II and XI , while arginine-298 and aspartic acid-326 are located in periplasmic and cytosolic loops 4 , respectively . - pubmed
We propose that the functional requirement of charged residues at both sides of the membrane and in two TM helices could be important to allow the passage of the Und - PP - linked saccharide substrate across the membrane . - pubmed
FYX-051 : a novel and potent hybrid - type inhibitor of xanthine oxidoreductase . - pubmed
4 -[ 5 -( Pyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile ( FYX-051 ) is a potent inhibitor of bovine milk xanthine oxidoreductase ( XOR ) . - pubmed
Steady - state kinetics study showed that it initially behaved as a competitive - type inhibitor with a K(i ) value of 5.7 x 10(-9 ) M , then after a few minutes it formed a tight complex with XOR via a Mo - oxygen - carbon atom covalent linkage , as reported previously ( Proc Natl Acad Sci USA 101:7931 - 7936 , 2004 ) . - pubmed
The FYX-051 - XOR complex decomposed with a half - life of 20.4 h , but the enzyme activity did not fully recover . - pubmed
This was found to be caused by XOR - mediated conversion of FYX-051 to 4 -[ 5 -( 2 - hydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile ( 2 - hydroxy - FYX-051 ) , as well as formation of 6 - hydroxy-4 -[ 5 -( 2 - hydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile ( dihydroxy - FYX-051 ) and 4 -[ 5 -( 2,6 - dihydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ]- 6 - hydroxypyridine-2 - carbonitrile ( trihydroxy - FYX-051 ) during prolonged incubation for up to 72 h. A distinct charge - transfer band was observed concomitantly with the formation of the trihydroxy - FYX-051 - XOR complex . - pubmed
Rho - kinase / nuclear factor - kappabeta / angiotensinogen axis in angiotensin II - induced renal injury . - pubmed
This work proposes a simple and sensitive solid substrate - room temperature phosphorimetry ( SS - RTP ) for the selective determination of carvedilol ( CV ) . - pubmed
The method is based on the sensitizing effect of sodium dodecyl benzene sulphonate ( SDBS ) on CV to activate the oxidation between NaClO and amaranth , resulting in the intense quenching of room temperature phosphorescence ( RTP ) of the system . - pubmed
Ranolazine , nitroglycerin , or saline ( control ) was injected into the left anterior descending ( LAD ) coronary artery or femoral artery ( 2 - mL bolus in 10 seconds ) . - pubmed
Pretreatment with prazosin ( 300 mug / kg IV ) allowed determination of alpha ( 1 )- adrenergic receptor involvement ( n=8 ) . - pubmed
Rapid intracoronary administration of ranolazine ( 0.048 mg / kg ) to achieve high local concentrations resulted in 91 + -11 % increase in LAD coronary artery flow and 39 + -7 % reduction in coronary vascular resistance ( both , P<0.0001 ) . - pubmed
Intracoronary nitroglycerin ( 100 mug ) increased LAD coronary artery flow by 112 + -25 % ( P=0.02 ) , but the effect lasted & lt;2 minutes ; mean arterial pressure decreased by 4 + -1 mm Hg ( P=0.01 ) . - pubmed
CONCLUSIONS : Intracoronary or intrafemoral ranolazine bolus exerts a marked , 2- to 3 - minute dilatory effect that is comparable to nitroglycerin in magnitude but more persistent , attributable primarily to alpha ( 1 )- adrenergic blockade . - pubmed
Tyrosine kinase inhibitors such as imatinib , dasatinib , and nilotinib interfere with ATP - binding pocket to inhibit BCR - ABL1 kinase . - pubmed
Treatment with MMF prevented the motor impairment and significantly reversed the changes in levels of malondialdehyde , glutathione , myeloperoxidase and nitric oxide . - pubmed
Vasopressin receptor antagonists such as lixivaptan may provide effective volume unloading . - pubmed
In the present study , Rmi1 was found to be enriched at the region close to an early - firing replication origin when replication forks were arrested near their origins in the presence of hydroxyurea . - pubmed
The local synthesis of type 2 deiodinase ( Dio2 ) promotes triiodothyronine ( T3 ) production and summer biology , whereas type 3 deiodinase ( Dio3 ) promotes T3 degradation and winter biology . - pubmed
Both the central hypointense signal area and the peripheral cogwheel - shaped hypointense signal band in the tumor were observed on the post - contrast T1WIs , in accordance with the necrosis observed in the photomicrographs following hematoxylin and eosin ( HE ) staining . - pubmed
Previous studies reported that specific alleles and single nucleotide polymorphisms ( SNPs ) in the human leukocyte antigen ( HLA ) region were associated with NOA in East Asians . - pubmed
CONCLUSION : In unresectable primary RCC tumors , changes in select CT parameters after 2 cycles of neoadjuvant sunitinib might be associated with the potential for surgical resection . - pubmed
At 15 C , the relationship between RS ( microl O ( 2 ) individual(-1 ) h(-1 ) ) and larval dry mass ( M(D ) , microg ) was equal to : R(S ) = 0 0057(+-0 0007 , + - s.e . ) - pubmed
In mid-2011 , the addition of direct - acting antiviral agents ( DAAs)-the protease inhibitors boceprevir ( Victrelis , Merck ) and telaprevir ( Incivek , Vertex)-to pegylated interferon alpha-2a / b and ribavirin revolutionized the treatment of HCV infection by increasing cure rates across all fibrosis scores in patients with genotype 1 HCV infection . - pubmed
Overall , the following parameters were found to significantly influence cellulose consumption in a consolidated bioprocess ( CBP ): ( 1 ) the glucose uptake rate of the culture , ( 2 ) the bacterial cell concentration , and ( 3 ) the nature of the cellulase enzyme system ( complexed or non - complexed ) . - pubmed
In 15 % of cases , FDG - PET findings are false positive ( FP ) with unclear consequences . - pubmed
Therefore , we retrospectively investigated the rate of unnecessary procedures due to FP results on FDG - PET . - pubmed
Interestingly , USP35 only associates with polarized mitochondria , and rapidly translocates to the cytosol during CCCP - induced mitophagy . - pubmed
The current study aims to synthesize and compare the effect of dexamethasone ( Dex ) release from 2 variants of Dex - loaded chitosan nanoparticles ( CSnp ) on the odontogenic differentiation of stem cells from apical papilla ( SCAP ) . - pubmed
Biomineralization of SCAP was significantly higher ( P & lt ; .05 ) in presence of Dex - CSnpII compared with that in Dex - CSnpI at 3 weeks . - pubmed
Alkaline phosphatase gene expression was significantly higher in the presence of Dex - CSnpII compared with Dex - CSnpI , with peak expression seen at 2 weeks ( P & lt ; .05 ) . - pubmed
The expression of odontogenic specific marker dentin matrix protein-1 was significantly higher in presence of Dex - CSnpII compared with Dex - CSnpI at 3 weeks ( P & lt ; .05 ) . - pubmed
CONCLUSIONS : Collectively , these data suggest that sustained release of Dex results in enhanced odontogenic differentiation of SCAP . - pubmed
During ischemia - reperfusion , AMPK activation modulates glucose and fatty acid metabolism , mitochondrial function , endoplasmic reticulum ( ER ) stress , autophagy , and apoptosis . - pubmed
Trametinib radiosensitises RAS- and BRAF - mutated melanoma by perturbing cell cycle and inducing senescence . - pubmed
Thus , we studied whether trametinib , a potent and selective allosteric inhibitor of MEK1 / 2 could improve the efficacy of RT . - pubmed
The effects of trametinib with and without radiation on protein levels of MEK effectors were measured by immunoblot analyses . - pubmed
Trametinib efficiently blocked RT - induced phosphorylation of ERK at nanomolar concentrations . - pubmed
A larger population of senescence - activated beta - galactosidase - positive cells was seen in the trametinib pretreated group , and this correlated with activation of two of the major mediators of induced senescence , p53 and pRb . - pubmed
CONCLUSION : Trametinib radiosensitised RAS-/RAF - mutated melanoma cells by inducing prolonged G1 arrest and premature senescence . - pubmed
Blood pressure , urinary albumin / creatinine ratio , and serum albumin and creatinine levels were measured . - pubmed
There were no significant differences in urinary albumin / creatinine ratio as well as serum albumin and creatinine levels in Model and Sham groups . - pubmed
Possible Role of HER-2 in the Progression of Prostate Cancer from Primary Tumor to Androgen Independence . - pubmed
The influence of HER-2 expression on the frequency of biochemical failure and effects of androgen blockade was determined . - pubmed
Men CPC ( + ) had a higher frequency of biochemical failure , but there was no difference in HER-2 expression of CPCs with the frequency of biochemical failure . - pubmed
After androgen blockade , men with HER-2 ( + ) positive secondary CPCs had a higher frequency of disease progression to castrate resistant disease . - pubmed
In the presence of androgens , there is no survival advantage of expressing HER-2 , but once biochemical failure has occurred and androgen blockade started , HER-2 positive cells are resistant to treatment , survive and grow leading to castration resistant disease . - pubmed
BACKGROUND : The phosphodiesterase-5 inhibitor sildenafil has been shown to attenuate delayed cerebral ischemia ( DCI ) and improve neurologic function in experimental subarachnoid hemorrhage ( SAH ) . - pubmed
Each underwent ( 15 ) O - PET imaging to measure global and regional CBF at baseline and post - sildenafil . - pubmed
RESULTS : Mean arterial pressure declined by 10 mm Hg on average post - sildenafil ( 8 % , p = 0.01 ) , while ICP was unchanged . - pubmed
There was no change in global CBF ( mean 34.5 + - 7 ml/100g / min at baseline vs. 33.9 + - 8.0 ml/100g / min post - sildenafil , p = 0.84 ) . - pubmed
Up - regulating the abscisic acid inactivation gene ZmABA8ox1b contributes to seed germination heterosis by promoting cell expansion . - pubmed
Here we show that increased mutation density at gene promoters can be linked to promoter activity and differential nucleotide excision repair ( NER ) . - pubmed
Cost - Effectiveness Analysis of Ugt1a1 Genotyping Before Colorectal Cancer Treatment with Irinotecan . - pubmed
Specifically , these studies have established that artemisinin resistance manifests as slow parasite clearance in patients and increased survival of early - ring - stage parasites in vitro ; is caused by single nucleotide polymorphisms in the parasite 's K13 gene , is associated with an upregulated " unfolded protein response " pathway that may antagonize the pro - oxidant activity of artemisinins , and selects for partner drug resistance that rapidly leads to ACT failures . - pubmed
Intensifying these efforts should help to forestall the spread of artemisinin and partner drug resistance from SEA to sub - Saharan Africa , where the world 's malaria transmission , morbidity , and mortality rates are highest . - pubmed
The inhibitor of canonical NFkappaB activation parthenolide inhibited the CCL19 and the early RANTES responses , but not the CCL21 or late RANTES responses . - pubmed
The gilts were fed one of the following three diets for 14 days : a basic diet for the control group ; the basic diet supplemented with ZEA - contaminated basic diet for the treatment 1 ( T1 ) group ; and the basic diet supplemented with fermented ZEA - contaminated basic diet by CK1 for the treatment 2 ( T2 ) group . - pubmed
The actual ZEA contents ( analyzed ) were 0 , 1.20 + - 0.11 , 0.47 + - 0.22 mg / kg for the control , T1 , and T2 diets , respectively . - pubmed
Net glucose and insulin production were calculated from portal - arterial differences x portal blood flow , and PDV O consumption was calculated as arterial - portal O differences x portal blood flow . - pubmed
Indications of chemical bond contrast in AFM images of a hydrogen - terminated silicon surface . - pubmed
The lotr1 mutants display altered localization of CASP1 , an essential protein for Casparian strip formation [ 9 ] , disrupted Casparian strips , ectopic suberization of endodermal cells , and low accumulation of shoot calcium ( Ca ) . - pubmed
Ectonucleoside triphosphate diphosphohydrolase 1 ( NTPDase1 ) degrades the purines ATP and ADP that are key regulators of inflammation and clotting . - pubmed
Prepared nanocomposites were characterized by means of FT - IR , 1H NMR , Zeta - potential measurements , TEM and EDX analyses . - pubmed
Replication of rs300774 , a genetic biomarker near ACP1 , associated with suicide attempts in patients with schizophrenia : Relation to brain cholesterol biosynthesis . - pubmed
This SNP also functioned as a trans - eQTL for genes related to cholesterol biosynthesis and the wnt - beta - catenin pathway ( p & lt;= 0.0001 ) . - pubmed
Further , co - expression analysis of candidate genes in brain suggested ACP1 is important to the regulation of a number of brain mechanisms linked to suicide , including cholesterol synthesis , beta - catenin - mediated signaling pathway , serotonin , GABA , and the stress response via ARHGAP35 ( p190rhogap ) , a repressor of glucocorticoid receptor ( NR3C1 ) transcription . - pubmed
SA application significantly improved freezing tolerance as evidenced by reduced ion - leakage and alleviated oxidative stress ( lower accumulation of O2 - and H2O2 ) following freeze - thaw stress treatments ( -6.5 , -7.5 , and -8.5 C ) . - pubmed
Improved freezing tolerance of SA - treated leaves was paralleled by increased proline and ascorbic acid ( AsA ) accumulation . - pubmed
A 9 - d cold acclimation ( CA ) treatment also improved leaf freezing tolerance ( compared to non - acclimated control ) and was accompanied by accumulation of SA and proline . - pubmed
Notably , the beneficial effect of SA on freezing tolerance was abolished when either H2O2- or NO - scavenger ( 1 muM N - acetylneuraminic acid , NANA or 100 muM hemoglobin , HB , respectively ) was added to SA as pretreatment . - pubmed
Glycosaminoglycan ( GAG ) enrichment is a dominant hallmark of early CAVD , but culture of valvular interstitial cells ( VICs ) in biomaterial environments containing pathological amounts of hyaluronic acid ( HA ) or chondroitin sulfate ( CS ) did not directly increase indicators of disease progression such as VIC activation or inflammatory cytokine production . - pubmed
Stretched samples had increased myocardial cGMP levels , increased phosphorylated vasodilator - stimulated phosphoprotein phosphorylation , as well as , increased titin phosphorylation , which was reduced by prior protein kinase G ( PKG ) inhibition ( PKGi ) . - pubmed
PT decay after stretching was significantly reduced by combined NP antagonism , NO synthase inhibition and NO scavenging , or by PKGi . - pubmed
However , glycated albumin is also affected by obesity and albumin , and it is desirable to make a comprehensive judgment with glycated haemoglobin , random plasma glucose and other glycaemic index . - pubmed
Meanwhile , ICS II repressed the down - regulation of calbindin protein induced by IBO . - pubmed
CONCLUSION : These results suggest that ICS II attenuates IBO - induced cognitive deficits , possibly via the regulation of calbindin expression and the inhibition of apoptotic response . - pubmed
In addition , the MAPK signaling pathway is implicated in the potential mechanisms of ICS II against IBO - induced excitotoxicity , indicating that ICS II is a promising compound for treatment of excitotoxicity - related diseases , including AD . - pubmed
There are three classes of drugs available for treatment : endothelin receptor antagonists ( ERA ) , drugs acting on nitric oxide pathway ( riociguat and phosphodiesterase type 5 inhibitors [ PDE5i ] ) , and drugs acting on prostacyclin pathway . - pubmed
Importantly , a significant modulation of several psoriasis - related inflammatory cytokines and chemokines was observed compared to the high dose of free EGCG ( p<0.05 ) . - pubmed
In the bronchoalveolar lavage fluid , GO and DADS inhibited the elevation in the counts of inflammatory cells , particularly neutrophils , which were induced in the CS and LPS ( CS + LPS ) group . - pubmed
Additionally , GO and DADS inhibited the phosphorylation of extracellular signal - regulated kinase and the expression of matrix metalloproteinase-9 in the lung tissues . - pubmed
Taken together , these findings indicate that GO and DADS could be a potential preventive agent in CS - induced airway inflammation . - pubmed
Isothiocyanates ( ITCs ) are chemoprotectives produced as the hydrolyzed product of GSLs by enzyme myrosinase . - pubmed
Induction of ARE ( antioxidant responsive element ) and ROS ( reactive oxygen species ) mediated pathway by Nrf2 controls the activity of nuclear factor - kappaB ( NF - kappaB ) . - pubmed
Antagonistic activity of BITC , PEITC and SFN against cancer was related with the direct / indirect interaction with Nrf2 and NF - kappaB protein . - pubmed
All three ITCs able to disrupts Nrf2 - Keap1 complex and translocate Nrf2 into the nucleus . - pubmed
BITC have the affinity to inhibit the NF - kappaB than SFN due to the presence of additional benzyl structure . - pubmed
We have also discussed the molecular interaction(s ) of the antagonistic effect of BITC , PEITC and SFN with Nrf2 and NF - kappaB to prevent cancer . - pubmed
The hole transport layer - free C - PSC with a structure of fluorine - doped tin oxide / compact TiO2 / mesoporous TiO2 / CH3NH3PbI3 - SWCNT / SWCNT - C gained a remarkable PCE of 15.73 % with an FF of 0.72 , accompanied by an outstanding stability of 90 days in the dark under high - humidity [ 65 + - 5 % relative humidity ( RH ) , 25 + - 5 C ] and high - temperature ( 75 + - 5 C , 25 + - 5 % RH ) conditions . - pubmed
rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes , such as paralogs of PR1 and PR5 , and other factors of the salicylic acid signaling pathway . - pubmed
Sinomenine retards LPS - elicited inflammation via down - regulating CCAT1 in HaCaT cells . - pubmed
Sinomenine pre - administration maintained cell viability , blocked apoptosis and relieved inflammation with the decrease in cleaved caspase-3 /- 9 and inflammatory factors . - pubmed
LPS - induced phosphorylation of p65 , IkappaBalpha and p38MAPK and overexpression of CCAT1 were precluded by sinomenine . - pubmed
CCAT1 overexpression , which per se induced inflammatory lesions , negated the positive effects of sinomenine with the restored phosphorylation of p65 , IkappaBalpha , and p38MAPK . - pubmed
The anti - inflammatory activity of sinomenine might be mediated by CCAT1 down - regulation . - pubmed
LC - MS analysis revealed that the extracts contained different classes of phenolics . - pubmed
Bone Marrow and Tumor Radiomics at 18F - FDG PET / CT : Impact on Outcome Prediction in Non - Small Cell Lung Cancer . - pubmed
Each tumor , its surrounding penumbra , and bone marrow from the L3 - L5 vertebral bodies was contoured on pretreatment FDG PET / CT images . - pubmed
At the end of induction treatment , the CR / PR rate was 43 6/50 9 % and 36 3/60 8 % in the R - CHOP and R - CVP groups ( P = 0 218 ) respectively . - pubmed
After a median follow - up of 67 , 66 , and 70 months , five - year EFS was 61 % vs. 56 % ( not significant ) , progression - free survival ( PFS ) was 71 % vs. 69 % ( not significant ) and overall survival ( OS ) was 84 % vs. 89 % in the R - CHOP vs. the R - CVP arm respectively . - pubmed
Butyrate supplementation suppresses arthritis in a Breg - dependent manner by increasing the level of the serotonin - derived metabolite 5 - Hydroxyindole-3 - acetic acid ( 5 - HIAA ) , which activates the aryl - hydrocarbon receptor ( AhR ) , a newly discovered transcriptional marker for Breg function . - pubmed
Thus , butyrate supplementation via AhR activation controls a molecular program that supports Breg function while inhibiting germinal center ( GC ) B cell and plasmablast differentiation . - pubmed
One - pot synthesis of glycyrrhetic acid polyglycosides based on grafting - from method using cyclic sulfite . - pubmed
Glycyrrhetic acid polyglycosides were synthesized in one - pot via cationic ring - opening condensation polymerization of cyclic sulfite ( 4 ) initiated by glycyrrhetic acid as an aglycon . - pubmed
To more effectively identify promising Nrf2 activators through the inhibition of Keap1 - Nrf2 PPI , a homogeneous time - resolved fluorescence resonance energy transfer ( TR - FRET ) assay was developed in this work by indirectly labeling the Keap1 Kelch domain protein with Tb - anti - His antibody as the donor and using , as the acceptor , fluorescein isothiocyanate ( FITC)-labeled 9mer Nrf2 peptide amide , the same fluorescent probe that was used in an earlier fluorescence polarization ( FP ) assay . - pubmed
Silencing of Nrf2 enhanced the degree of apoptosis of EPCs as well as resulted in the impairment of EPC functions through inducing the promotion of ( reactive oxygen species ) ROS production . - pubmed
Marein treatment decreased hypoxia inducible factor-1alpha ( HIF-1alpha ) , peroxisome proliferator activated receptor gamma ( PPARgamma ) , medium chain acyl - coenzyme A dehydrogenase , glucose transporter-4 , and glycerol-3 - phosphate acyltransferase protein expressions , and increased PPARalpha , fatty acid transport protein-1 , carnitine palmitoyltransferase-1 , and pyruvate dehydrogenase kinase-4 protein expressions . - pubmed
TRO was faster for the LAP - ST ( mean = 6.8 min ; SE = 4.9 ) than standard RUL ECT ( mean = 15.5 min ; SE = 6.5 ) . - pubmed
Our results revealed NEO and its constituents as potent and efficient Cx43 GJ inhibitors acting by slow gating mechanism . - pubmed
GLP-1 regulation by food proteins and protein hydrolysates . - pubmed
Glucagon - like peptide 1 ( GLP-1 ) is an enterohormone with a key role in several processes controlling body homeostasis , including glucose homeostasis and food intake regulation . - pubmed
We review the literature on the GLP-1 secretory effects of protein and protein hydrolysates , and the mechanisms through which they exert these effects . - pubmed
Crude extracts of the transport - deficient mutant strain uc-5 pyr-1 were shown to have uridine 5 '- monophosphate pyrophosphorylase activity comparable to that of the wild - type strain , suggesting that uracil uptake in Neurospora does not occur by a group translocation mechanism involving phosphoribosylation . - pubmed
Specificity studies of uridine 5 '- monophosphate pyrophosphorylase indicated that phosphoribosylation was not an important determinant of the specificity of uracil uptake . - pubmed
Rabbit muscle pyruvate kinase has been shown to catalyze the decarboxylation of oxalacetate ( Creighton , D.J. - pubmed
Phosphoenol - alpha - ketobutyrate , an analog of the substrate phosphoenol - pyruvate , inhibits decarboxylase and kinase competitively and with nearly identical Ki values ( 5.7 micron and 4.8 micron , respectively ) . - pubmed
But , effects of the following modifiers suggest that different amino acid residues at that site participate in the two reactions : phenylalanine inhibition and fructose 1,6 - bisphosphate activation are more effective with the decarboxylase ; iodoacetamide preferentially inactivates decarboxylase while trinitrobenzenesulfonate preferentially inactivates kinase . - pubmed
The purified enzyme was homogeneous as judged by disc gel electrophoresis and was most active at pH 7.8 using formaldehyde as a substrate . - pubmed
The enzyme was also active toward acetaldehyde , propionaldehyde , glyoxal , and pyruvaldehyde , though the reaction rates were low . - pubmed
The enzyme was markedly inhibited by Ni2 + , Pd2 + , Hg2 + , p - chloromercuribenzoate , and phenylmethanesulfonyl fluoride . - pubmed
The molecular weight of the enzyme was estimated to be 150,000 by the gel filtration method , and analysis by SDS - polyacrylamide gel electrophoresis indicated that the enzyme was composed of two subunit monomers . - pubmed
The enzyme catalyzed the oxidation of formaldehyde accompanied by the stoichiometric reduction of NAD + , but no reverse reaction was observed . - pubmed
[ Effect of labor on plasma levels of chorionic somatomammotropin ( CS ) , estradiol ( E2 ) , progesterone ( Pr ) and cortisol ; behavior of these levels in the first hours of the puerperium ] . - pubmed
These studies have shown that the target of this nalidixic acid effect is dictated by the recB and recC genes and may be due to the binding of nalidixic acid to exonuclease V. - pubmed
Growth factors elicit increases in both the phosphoserine and the phosphotyrosine content of the PLC - gamma 1 isozyme . - pubmed
PLC - gamma 1 contains three tyrosine phosphorylation sites , which have been identified as residues 771 , 783 and 1254 . - pubmed
Phosphorylation of tyrosine residues is sufficient to increase the catalytic activity of PLC - gamma 1 , though other proteins may modulate this activation . - pubmed
However , the role of growth factor - enhanced phosphorylation of serine residues on PLC - gamma 1 remains obscure . - pubmed
In vitro studies of PLC - gamma 1 , recovered from growth factor - treated cells , indicate that activation by tyrosine phosphorylation is not due to increased sensitivity to Ca2 + , a required co - factor , but is reflected in altered kinetic constants , i.e. - pubmed
Disappearance of a highly unusual clone , 46,XY , del(7)(p12),t(9;22)(q34;q11 ) in chronic myeloid leukemia after treatment with recombinant interferon and cytosine arabinoside . - pubmed
Conventional chemotherapy with hydroxyurea and then with recombinant interferon - alpha ( rIFN - alpha ) did not reduce the population of either subclone . - pubmed
However , after treatment with a combination of rIFN - alpha and low - dose cytosine arabinoside ( LoDac ) continuously infused subcutaneously ( s.c . ) - pubmed
The Ki values for these nucleoside inhibitors were similar to their reported Km values as permeants of the nucleoside transporter . - pubmed
To test this hypothesis , the endoplasmic reticulum retention sequence Lys - Asp - Glu - Leu ( KDEL ) was added to the C - terminus of the wildtype TGF - beta 1 coding sequence , and to a construct in which mutagenesis of two cysteine residues in the precursor pro region results in the synthesis and secretion of active , as opposed to latent , TGF - beta . - pubmed
These and other observations demonstrate that in a purely " postsynaptic " system , short - term potentiation can be induced and is mediated via NMDA receptors whereas the potentiated current is carried via non - NMDA glutamate receptor channels . - pubmed
The observations of cell surface antigen expression were paralleled by analysis of glycolipids isolated from the cells by thin - layer chromatography . - pubmed
This analysis , in which the new monoclonal antibodies VINIS-56 and VIN-2PB-22 were included , also revealed expression of gangliosides GD3 and GD2 in all differentiated cultures , albeit at much lower levels following HMBA exposure than following retinoic acid or BUdR - exposure . - pubmed
The following results were observed : 1 ) vasoactive intestinal polypeptide and enkephalin did not influence mucociliary activity ; 2 ) bombesin had only a slight accelerating effect on the mucociliary activity at doses of 0.1 to 10 micrograms / kg ; and 3 ) substance P markedly accelerated the mucociliary activity in a dose - dependent manner in the dose range 0.01 to 10 micrograms / kg , the maximal increase being about 50 % . - pubmed
The effect of substance P was atropine - resistant , and probably acted directly on the mucosa . - pubmed
Cilazapril induced a pronounced increase of plasma renin activity and angiotensin I concentrations , whereas after propranolol both parameters decreased . - pubmed
After the pharmacological challenges with angiotensin I and isoprenaline specific effects of the ACE inhibitor and beta - adrenoceptor blocker were found respectively . - pubmed
At rest blood pressure was lowered by both drugs , but total peripheral resistance increased after propranolol and decreased after cilazapril . - pubmed
Essentially fatty - acid - free or essentially fatty - acid- and globulin - free BSA stimulated RTE cell colony formation at low concentrations ( less than 2.5 to 5 mg BSA / ml ) but resulted in concentration - dependent decreases in colony - forming efficiency at higher concentrations . - pubmed
Finally , commercially available fraction V BSA prepared by heat shock , dialysis , charcoal treatment , and deionization was stimulatory at low concentrations but inhibitory at high concentrations . - pubmed
These results suggest that a massive influx of sodium into the muscle fibers through their cut ends results in maximal stimulation of the Na +- K +- ATPase of the T - tubular membrane leading to increasing Na + concentration in the lumen of T - tubules followed by influx of Cl- to maintain the electrical gradient . - pubmed
The anterior pituitary gland , in turn , appears to require this episodic GnRH stimulation in order to secrete the gonadotropins LH and FSH in a physiologic fashion . - pubmed
Replacement regimens of GnRH which employ an episodic pattern of delivery correct the deficiency of endogenous GnRH secretion and stimulate gonadotropin secretion , gonadal steroidogenesis and gametogenesis . - pubmed
However , continuous occupancy of the GnRH receptor by native GnRH and/or its long - acting analogs paradoxically inhibits pituitary gonadotropin secretion . - pubmed
GnRH analogs have been synthesized which exhibit greater affinity and prolonged occupancy on the GnRH receptor and can produce pituitary desensitization of gonadotropin secretion . - pubmed
As regards the presence of carbonic anhydrase in the different types of gastric glandular cells , in addition to the already known function of the enzyme in hydrochloric acid secretion in the oxyntic and oxyntic - peptic cells , we suggest an interpretation of carbonic anhydrase presence observed in many chief cells . - pubmed
In the case of HDL cholesterol , the differences were 0.23 mmol / l among the men and 0.34 mmol / l among the women , explaining in large part the differences of the mean levels of total cholesterol . - pubmed
In serum samples phospholipase A2 , leukotriene C4 , prostaglandin E2 and gamma glutamyl transpeptidase were measured . - pubmed
These results suggest that diabetes results in a loss of myocardial sensitivity to insulin which seems to occur as early as 3 days after induction of diabetes with STZ . - pubmed
Chemical modification of bovine brain tubulin with the guanine nucleotide affinity analogue 5 '- p - fluorosulfonylbenzoylguanosine . - pubmed
Bovine brain tubulin purified in the absence of GTP and MgCl2 , reacts with 5 '- p - fluorosulfonylbenzoylguanosine ( 5 '- FSBG ) 2 , an affinity analog of GTP and two moles of the reagent are incorporated per mole of tubulin at 0 degree C. 5 '- FSBG is unable to promote the polymerization of tubulin into microtubules . - pubmed
Tubulin modified with 5 '- FSBG lost two sulfhydryl groups per mole of tubulin and reduction with beta - mercaptoethanol led to the loss of the 2 moles of FSBG that had been incorporated . - pubmed
These data are interpreted on the basis that the modification of tubulin by 5 '- FSBG proceeds via a thiosulfonate intermediate between the analogue and a reactive thiol group at or near that portion of the GTP binding site of tubulin where the phosphate moiety of GTP binds . - pubmed
Stepwise phosphorolysis with polynucleotide phosphorylase : a novel method for sequence analysis of oligoribonucleotides . - pubmed
[ Trimepranol , a new Czechoslovak sympatholytic beta receptor blockader . - pubmed
The effect of diethyaminoethyl diphenylpropylacetic acid ( SKF 525 - A ) on uridine 5 '- pyrophosphate glucuronyltransferase . - pubmed
The amino acid and nucleotide sequences encoded by the unmutated gene predict that a guanine - to - adenine transition is responsible for the glycine - to - aspartic acid mutation at codon 244 and would also result in the loss of a HaeIII site . - pubmed
Substitution of the hydroxyl group on C7 of cholic acid by a benzamido group leads to a derivative with inhibiting quality for the inward transport of both bile acids and phallotoxins by isolated liver cells . - pubmed
The latter compound was used for affinity labeling of liver plasma membranes in order to detect chemically modified proteins involved in the transport of bile acids . - pubmed
The 67 kDa protein could be found in the aqueous phase after phase separation in Triton X-114 . - pubmed
Aminoacyl - tRNA synthetase fails to activate benzo ( a ) pyrene-4,5 - dihydrodiol for binding to protein in vitro . - pubmed
Response to fibrinolytic activity and factor VIII - related antigen to stimulation with desmopressin in hyperlipoproteinemia . - pubmed
A subnormal activity was invariably found when serum triglyceride concentration was above 8 mmol / L. The defect can be attributed to low levels of extrinsic plasminogen activator . - pubmed
2,2 '- Dipyridyl enhanced the production of chemiluminescence in the presence of FeSO4 , ferritin and haemosiderin but not FeCl3 or horseradish peroxidase . - pubmed
Nitrilotriacetic acid ( NTA ) enhanced chemiluminescence in the presence of both FeSO4 and FeCl3 but not ferritin or haemosiderin . - pubmed
GLUT-2 gene transfer into insulinoma cells confers both low and high affinity glucose - stimulated insulin release . - pubmed
We found that the loss of glucose sensing in these cells was correlated with the loss of expression of GLUT-2 and glucokinase . - pubmed
GLUT-2 expressing cells also exhibited a larger response to the combination of 5 mM glucose + 1 microM forskolin than untransfected cells ( 7.9 versus 1.6 - 2.7 - fold , respectively ) . - pubmed
Preincubation of GLUT-2 expressing intermediate passage RIN cells with 2 - deoxyglucose to inhibit low Km hexokinases resulted in a glucose - stimulated insulin secretion response that was shifted toward the physiologic range . - pubmed
These studies indicate that GLUT-2 expression confers both a high and low affinity glucose - stimulated insulin secretion response to intermediate passage RIN cells . - pubmed
Verapamil is an antagonist of L - type Ca2 + channels , and part of its binding site is located in the sixth transmembrane segment ( S6 ) in the fourth repeat of the protein . - pubmed
Verapamil also blocks K + channels , which are members of the same supergene family as Ca2 + channels . - pubmed
S6 of fHK has significant homology to that portion of the verapamil binding site identified in Ca2 + channels , and S6 is thought to form part of the inner mouth of K+ channel pores . - pubmed
Hypertriglyceridemic effect of fructose is mainly due to an increase in hepatic production of VLDL . - pubmed
Cis - acting element named PKL - III in the 5'-flanking region of this gene is shown to be responsive to dietary fructose as well as to dietary glucose . - pubmed
Overexpression of calreticulin increases intracellular Ca2 + storage and decreases store - operated Ca2 + influx . - pubmed
The widely distributed and highly conserved Ca ( 2 +)-binding protein calreticulin has been suggested to play a role as a Ca2 + storage protein of intracellular Ca + stores . - pubmed
The total cellular Ca2 + content of calreticulin - overexpressing and control cells , as assessed by equilibrium 45Ca + 2 uptake , was 141 + /- 8 and 67 + /- 6 pmol of Ca2+/10(6 ) cells , respectively ( i.e. - pubmed
a 2.1 + /- 0.2 - fold increase in the Ca2 + content of calreticulin - overexpressing cells ) . - pubmed
In contrast , thapsigargin - induced Ca2 + and Mn2 + influxes from the extracellular space were markedly diminished in calreticulin - overexpressing cells , suggesting an active involvement of calreticulin in the regulation of store - operated Ca2 + influx . - pubmed
Rhizobium " hedysari " HCNT1 is an unclassified rhizobium which contains a nitric oxide - producing nitrite reductase but is apparently incapable of coupling the reduction of nitrite to energy conservation . - pubmed
The gene encoding the nitrite reductase , nirK , has been cloned and sequenced and was found to encode a protein closely related to the copper - containing family of nitrite reductases . - pubmed
Unlike other members of this family , nirK expression in HCNT1 is not dependent on the presence of nitrogen oxides , being dependent only on oxygen concentration . - pubmed
This loss of sensitivity suggests that the product of nitrite reductase , nitric oxide , is responsible for inhibition of oxygen respiration . - pubmed
By using a newly developed chemically modified electrode to detect nitric oxide , it was found that nitrite reduction by HCNT1 produces significantly higher nitric oxide concentrations than are observed in true denitrifiers . - pubmed
The capacity to generate such large concentrations of freely diffusible nitric oxide as a consequence of nitrite respiration makes HCNT1 unique among bacteria . - pubmed
A beta production in the TGN persisted even under conditions in which formation of nascent post - TGN vesicles was inhibited by addition of guanosine 5 '- O -( 3 - thiotriphosphate ) , a nonhydrolyzable GTP analogue , or by omission of cytosol . - pubmed
The kinetic pattern obtained from all groups , shows a significant , five - phased curve : phase I ( 2 - 6 days post - vaccination ) shows a slow increase in positive Anthraxin skin reactions . - pubmed
During phase V ( 91 - 365 days post - vaccination ) there is a continuous decrease in positive Anthraxin skin reactions . - pubmed
The authors studied 50 nondiabetic persons who had completed a euglycemic hyperinsulinemic clamp protocol in addition to a 75 - g oral glucose tolerance test and other measures of the coronary risk profile . - pubmed
Factor 1 consisted of positive loadings for uric acid , systolic and diastolic blood pressure , triglyceride concentration , and waist girth and negative loadings for HDL cholesterol and the rate of insulin - mediated glucose disposal ( M , in milligrams per kilogram of body weight per minute ) . - pubmed
M also loaded on factor 2 , along with fasting insulin and glucose concentrations , diastolic blood pressure , and waist girth . - pubmed
Placing a number of different peptides at the COOH termini of a secreted protein and two proteins with type II membrane anchors resulted in their TAP - independent presentation . - pubmed
These findings demonstrate that the secretory compartment possesses proteases able to liberate COOH - terminal antigenic peptides from virtually any context , entirely consistent with a role for these proteases in the processing of TAP - transported antigenic peptide precursors . - pubmed
Short - chain fatty acids inhibit intestinal trefoil factor gene expression in colon cancer cells . - pubmed
The human ITF promoter contained an 11 - residue consensus sequence with high homology to the butyrate response element of the cyclin D1 gene . - pubmed
We conclude that butyrate inhibits ITF expression in colon cancer cells and that this effect may be mediated transcriptionally and independently of its effects on differentiation . - pubmed
The bispecific ligand - antibody conjugate described in this study is a chemical conjugate between an anti - CD3 monoclonal antibody ( MAb ) and folic acid , the ligand for a high - affinity surface receptor expressed on the surface of choroid plexus tumors . - pubmed
Phorbol 12 - myristate 13 acetate ( PMA ) , a potent PKC activator , at 25 nM inhibited the currents through GABArho2 receptors , which have one consensus phosphorylation site by PKC in the predicted intracellular loops . - pubmed
The inhibitory effect of PMA was also observed after removing each consensus phosphorylation site in both GABArho1 and rho2 receptors by site - directed mutagenesis . - pubmed
New minimal substrate structural requirements in the enzymatic peroxidation of alkenes with soybean lipoxygenase . - pubmed
Substance P - mediated [ Ca2 +]i responses involved generation of inositol 1,4 , 5 - trisphosphate and release of Ca2 + from thapsigargin - sensitive stores , whereas mechanically induced responses were partially ( 29 % ) dependent on La3 + -sensitive influx of extracellular Ca2 + and partially on release of intracellular Ca2 + from thapsigargin - insensitive stores gated by ryanodine receptors . - pubmed
In the later case , the underlying mechanism may involve capacitative Ca2 + entry channels that are activated after mechanical stimulation but not by substance P. - pubmed
SERMs offer post - menopausal women many of the advantages of estrogen replacement while mitigating some of the disadvantages , particularly the concern over breast cancer . - pubmed
Heterogeneous long chain acyl - CoA synthetases control distribution of individual fatty acids in newly - formed glycerolipids of neuronal cells undergoing neurite outgrowth . - pubmed
Cells treated with nerve growth factor ( NGF ) were labeled with [ 3H ] glycerol , [ 3H ] oleic acid ( OA ) or [ 3H ] arachidonic acid ( AA ) in the presence of other unlabeled fatty acids of endogenous or exogenous origin . - pubmed
Triacsin C ( 4.8 microM ) , an inhibitor of acyl - CoA synthetase , decreased the incorporation of exogenous [ 3H ] OA into glycerolipids by 30 - 90 % , and increased by about 60 % the accumulation of free [ 3H ] OA in the cells . - pubmed
Activities of the LCAS that is specific for AA ( ACS ) , or of the non - specific LCAS which activates OA and other fatty acids ( OCS ) , were much higher in microsomal and cytoplasmic fractions than in mitochondria or nuclei . - pubmed
The Vmax and Km values of ACS and OCS in microsomes were 12 and 0.7 nmol / min / mg protein and 70 and 37 microM , respectively ; and in cytoplasm , 6 and 0.6 nmol / min / mg protein and 38 and 60 microM , respectively . - pubmed
Triacsin C ( 2 - 33 microM ) did not affect ACS activity in microsomal or cytoplasmal fractions , but inhibited OCS activities dose - dependently and competitively : IC50 and apparent Ki values were 13.5 microM and 14 microM in microsomes , and 3.8 microM and 4 microM in cytoplasm . - pubmed
NGF stimulated the activities of the LCASs , and , consistently , the incorporation of the various fatty acids into glycerolipids . - pubmed
These data indicate that LCASs are heterogeneous with respect to their intracellular locations , substrate specificities , kinetic characteristics and sensitivities to triacsin C ; and that this heterogeneity affects the extents to which individual fatty acids are utilized to form glycerolipids . - pubmed
To clarify whether the sialyl glycoconjugates work as functional molecules in the process of eosinophil degranulation , we examined the localization of sialyl residues after degranulation stimulated with calcium ionophore A23187 or interleukin-5 . - pubmed
Risk assessment based on a clinical score system , myocardial perfusion scintigraphy single photon emission computed tomography ( SPECT ) at rest and during adenosine stress , echocardiography , radionuclide angiography , symptom - limited exercise stress test , and 24 - h Holter ECG recording with ST - analysis and analysis of heart rate variability ( HRV ) were performed 5 - 8 days after hospital admission . - pubmed
Analysis of tyrosine phosphorylation - dependent protein - protein interactions in TrkB - mediated intracellular signaling using modified yeast two - hybrid system . - pubmed
Activated receptor tyrosine kinases induce a large number of tyrosine phosphorylation - dependent protein - protein interactions through which they mediate their various ligand - exerted functions including regulation of proliferation , differentiation and survival . - pubmed
TrkB receptor tyrosine kinase activated by binding of brain - derived neurotrophic factor ( BDNF ) also stimulates various protein interactions in a tyrosine phosphorylation - dependent manner in neuronal cells . - pubmed
To examine tyrosine phosphorylation - dependent interactions stimulated by active TrkB , we developed a modified yeast two - hybrid system , which we call the yeast two - and - a - half - hybrid system . - pubmed
Using this system with Shp2 as the bait , we demonstrated that Shp2 interacts directly with BIT / SHPS-1 ( also called SIRP ) and Grb2 depending on tyrosine phosphorylation mediated by TrkB. - pubmed
We found that fibroblast growth factor receptor substrate 2beta ( FRS2beta ) , also called SNT2 , interacts with Shp2 dependently on TrkB - mediated tyrosine phosphorylation of FRS2beta / SNT2 . - pubmed
Therefore , we show that the two - and - a - half - hybrid system is a powerful tool for studying tyrosine phosphorylation - dependent protein - protein interactions in intracellular signaling pathways stimulated by TrkB receptor tyrosine kinase . - pubmed
Hormones exhibit a wide range of biological activities on the skin , with major effects caused by growth hormone / insulin - like growth factor-1 , neuropeptides , sex steroids , glucocorticoids , retinoids , vitamin D , peroxisome proliferator - activated receptor ligands , and eicosanoids . - pubmed
Inhibition of actin polymerization with cytochalasin A resulted in similar phenotypes , suggesting that Scar - mediated polymerization of the actin cytoskeleton was important in the regulation of these processes . - pubmed
Possible involvement of endothelin-1 and nitric oxide in the pathogenesis of proliferative diabetic retinopathy . - pubmed
Endothelin-1 and NO metabolites were measured by radioimmunoassay and high - performance liquid chromatography based on the Griess method , respectively . - pubmed
CONCLUSION : These results indicate that ET-1 and NO may be related in the pathogenesis of PDR . - pubmed
Cellular effects of purvalanol A : a specific inhibitor of cyclin - dependent kinase activities . - pubmed
In synchronized cells , purvalanol A induced a reversible arrest the progression in G1 and G2 phase of the cell cycle , but did not prevent the completion of DNA synthesis in S - phase cells . - pubmed
The cell contents of cyclins D1 and E were lower in cells incubated with purvalanol A compared to controls , but the level of the cdk inhibitory protein p21(WAF1 / CIP1 ) was increased , indicating that the drug did not cause a general inhibition of gene expression . - pubmed
This compound , however , caused an inhibition of the estradiol - induced expression of an integrated luciferase gene , suggesting that cdk or related enzymes may participate in the regulation of the activity of certain promoters . - pubmed
We found that Tax - mediated apoptosis in response to UV irradiation was inhibited by Bcl-2 and Bcl - X ( L ) overexpression and by treatment with the caspase inhibitor z - VAd - FMK . - pubmed
The phosphorylation of CREB , ATF1 and MAPKAP - K2 were inhibited by SB203580 , a p38MAPK inhibitor , but not by PD98059 , an ERK kinase inhibitor . - pubmed
OBJECTIVES : To compare the in vitro scleral permeability of carboplatin using either a fibrin sealant or a balanced salt solution ( BSS ) vehicle and to measure in vivo ocular tissue levels following subconjunctival injection of carboplatin in fibrin sealant or BSS . - pubmed
RESULTS : In vitro carboplatin in fibrin sealant had a peak permeability constant of 13.7 + /- 2.3 x 10(-6 ) cm / s ; carboplatin in BSS , 27.0 + /- 1.7 x 10(-6 ) cm / s. After 24 hours , 33.2 % + /- 1.8 % of the carboplatin was retained in the fibrin sealant , while 5.5 % + /- 1.0 % was retained in the BSS . - pubmed
In vivo subconjunctival injection of carboplatin in fibrin sealant vehicle achieved 11.83 + /- 5.16 microg / mL in the vitreous at 1(1/2 ) hours and 0.03 + /- 0.06 microg / mL in the vitreous at 2 weeks . - pubmed
CONCLUSION : Fibrin sealant provided a more controlled and localized release of carboplatin and delivered carboplatin to the ocular tissues for up to 2 weeks . - pubmed
CLINICAL RELEVANCE : This study reports the use of fibrin sealant as a subconjunctival delivery vehicle for carboplatin , and quantifies ocular drug levels achieved in an animal model . - pubmed
The purpose of the present study was to investigate the effects of an ECE inhibitor , CGS 26303 , on hemolysate - induced injury of cerebral vessel endothelial cells as well as the production of ET-1 from these cells . - pubmed
Treatment with CGS 26303 inhibited the hemolysate - induced increases in the levels of ET-1 and big ET-1 and reduced endothelial cell injury . - pubmed
During MPTP administration , phosphorylation of p - Jun was increased in the substantia nigra , and MKK4 was increased both in the striatum and substantia nigra . - pubmed
Nucleotide - based amplification method is an important system for the identification of genomic modified foods ( GMF ) . - pubmed
In the potassium channel KcsA and in the cation channel gramicidin , permeant selectivity and mobility are determined by the proteinaceous matrix via hydrogen bonding , charge - dipole , and dipole - dipole interactions . - pubmed
Se , Zn and Cu were measured in plasma together with glutathione peroxidase ( GSH - Px ) activity , and lipid peroxidation ( thiobarbituric acid - reactive substances -- TBARS ) . - pubmed
A radiophotoluminescent ( RPL ) glass rod dosimeter ( GRD ) and a small active volume p - type silicon diode detector ( stereotactic field detector , SFD ) were used for the measurement of Gamma - Knife output factors . - pubmed
The immunophenotypic profile of the two subtypes was indistinguishable concerning the presence or absence of these antigens , including the absence of reactivity for the HLA - DR antigen . - pubmed
For both R689Q and M731 T , sensitivity to vanadate inhibition is decreased , suggesting that the steady - state E(1 ) & lt;==> ; E(2 ) poise of the enzyme is shifted toward E(1 ) . - pubmed
It is suggested that the disease phenotype is the consequence of lowered molecular activity of the alpha2 pump isoform due to either decreased K(+ ) affinity ( T345A ) or catalytic turnover ( R689Q and M731 T ) , thus causing a delay in extracellular K (+ ) clearance and/or altered localized Ca ( 2 + ) handling / signaling secondary to reduced activity in colocalized Na(+)/Ca ( 2 + ) exchange . - pubmed
In an example application , PG-594 was added to simulated milk samples to study effects of heat and beta - lactoglobulin ( beta - LG ) on the distribution of PG . - pubmed
Before heating , about one - third of the PG-594 remained soluble in the whey fraction ( supernatant ) whereas the rest became associated with the casein micelle . - pubmed
Heat - induced PG-594 binding to micelles in whey - protein - free systems was evidenced by a decrease of PG-594 from 31 to 15 % in the whey fraction accompanied by an increase of PG-594 from 69 to 85 % in casein micelle fractions . - pubmed
Incubation of the casein micelles with the reducing agent beta - mercaptoethanol revealed that disulfide bonds formed between PG and casein or between PG and casein - bound beta - LG are the mechanisms for heat - induced PG binding to casein micelles . - pubmed
Theoretically important findings are : 1 ) when heated , serum PG is capable of covalently binding to micellar casein or complexing with beta - LG in whey and then coadhering to micelles , and 2 ) PG that associated with micellar casein through lysine binding sites before heating is capable of developing heat - induced disulfide bonds with casein . - pubmed
The fact that the main mediators involved are sulfidoleukotrienes ( LTC4 , LTD4 , LTE4 ) and that the drugs responsible all inhibit cyclooxygenase-1 ( COX-1 ) , pointed to a pharmacogenetic abnormality of arachidonic acid metabolism . - pubmed
The splanchnic bed produces cortisol at rates approximating extraadrenal tissues by converting cortisone to cortisol via the 11beta - hydroxysteroid dehydrogenase ( 11beta - HSD ) type 1 pathway . - pubmed
Fasting glucose and insulin concentrations did not differ on the meal and saline study days . - pubmed
Glucose and insulin concentrations increased after meal ingestion , peaking at 11.0 + /- 1.0 mmol / l and 451 + /- 64 pmol / l , respectively , at 45 min , then fell to baseline thereafter . - pubmed
In contrast , glucose and insulin concentrations slowly fell to 5.1 + /- 0.1 mmol / l and 27 + /- 6 pmol / l during the 6 h of observation on the saline study day . - pubmed
We conclude that ingestion of a mixed meal does not alter either splanchnic cortisol production or the conversion of D4 cortisol to D3 cortisol or , therefore by implication , flux via the splanchnic 11beta - HSD type 1 pathway . - pubmed
Enantiomer separation of amino acids in immunoaffinity micro LC - MS . - pubmed
Chiral immunoaffinity microbore columns were directly interfaced with MS detection , and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti - D - amino acid antibody onto silica . - pubmed
The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 x 150 mm column , while an increase in column length enabled separation of more weakly bound compounds . - pubmed
For the first time , simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody - based chiral stationary phases using extracted ion chromatograms . - pubmed
Synthesis , dihydrofolate reductase inhibition , antitumor testing , and molecular modeling study of some new 4 ( 3H )- quinazolinone analogs . - pubmed
After blending the products with polyurethane ( PU ) and casting the result as film , the PEO segments migrated to the surface of the blend and the PCL segments acted as an anchor to fix the co - polymer on PU matrix , while the PEO segments provided PU the hydrophibility to prevent the fibrinogen adsorption on PU . - pubmed
Biosynthesis of isoprenoids : studies on the mechanism of 2C - methyl - D - erythritol-4 - phosphate synthase . - pubmed
2C - Methyl - D - erythritol-4 - phosphate synthase , encoded by the ispC gene ( also designated dxr ) , catalyzes the first committed step in the nonmevalonate isoprenoid biosynthetic pathway . - pubmed
Hydroxyacetone , the putative second fragment expected from the retroaldol cleavage , was not incorporated into the enzyme product . - pubmed
In contrast to other reports , IspC did not catalyze the isomerisation of 1 - deoxy - D - xylulose 5 - phosphate to give 1 - deoxy - L - ribulose 5 - phosphate under any conditions tested . - pubmed
The patient carrying the last duplication presents sex reversal , Peters ' anomaly and renal cysts and the duplication is located 4Mb away from the HSD17B1 gene , coding a key enzyme of testosterone biosynthesis . - pubmed
Apixaban , an oral , direct inhibitor of activated Factor Xa . - pubmed
Apixaban is an oral , direct Factor Xa inhibitor that is being developed by Bristol - Myers Squibb Co and Pfizer Inc. Apixaban is currently undergoing phase III clinical trials for cerebrovascular ischemia , deep vein thrombosis and lung embolism , and phase II clinical trials for coronary artery disease . - pubmed
This was performed by direct analysis of the ability of U1 to bind to wild - type and mutant donor sites , by engineering an in vitro splicing system to directly evaluate the functional importance of U1 snRNA base pairing with the exon 29 donor site , and by coexpression of mutant U1 snRNP molecules to try to rescue exon 29 inclusion in vivo . - pubmed
The present study investigates the consequences of cigarette smoke exposure on ciliary function and transepithelial chloride ( Cl (- ) ) secretion , a major determinant of ASL . - pubmed
Effects on ciliary beat frequency ( CBF ) and Cl (- ) secretion were investigated using pharmacologic manipulation . - pubmed
Forskolin - stimulated CBF was also significantly reduced when acutely exposed to CSC ( 5.64 + /- 0.06 Hz vs. control 7.15 + /- 0.18 Hz ) . - pubmed
For the rapid detection of the ethanol , pH and rest sugar in red wine , infrared ( IR ) spectra of 44 wine samples were analyzed . - pubmed
The models were used to estimate the contents of ethanol , pH and rest sugar in red wine samples for both in calibration set and predicted set . - pubmed
The results indicate that the r and RMSEP for the prediction of ethanol content , pH and rest sugar content are 0.953 , 0.983 and 0.994 , and 0.161 , 0.017 and 0.181 , respectively . - pubmed
The addition of immunosuppressive therapy , such as rituximab , to ITI may improve outcomes , although the optimal approach to combined ITI / immunosuppression has not been established . - pubmed
Estrogen receptors alpha ( ER - alpha ) and beta ( ER - beta ) play distinct biological roles in onset and progression of hormone - responsive breast cancer , with ER - beta exerting a modulatory activity on ER - alpha - mediated estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood . - pubmed
This probably occurs by correcting diabetes - linked increased clearance of thiamine , maintaining activity and expression of thiamine pyrophosphate - dependent enzymes that help counter the adverse effects of high glucose concentrations - particularly transketolase . - pubmed
We have conducted a site - specific saturation mutagenesis study of H181 and H184 of flavoprotein pentaerythritol tetranitrate reductase ( PETN reductase ) to probe the role of these residues in substrate binding and catalysis with a variety of alpha , beta - unsaturated alkenes . - pubmed
In addition , many mutants exhibited a switch in reactivity to predominantly catalyse nitro reduction , as opposed to C C reduction . - pubmed
The multiple binding conformations of hydroxy substituted nitro - olefins in PETN reductase were examined by using both structural and catalytic techniques . - pubmed
Serum uric acid ( UA ) and creatine phosphokinase were evaluated . - pubmed
The levels of creatine phosphokinase and F ( 2 )- isoprostanes decreased after exercise . - pubmed
Prevention of benzyl alcohol - induced aggregation of chymotrypsinogen by PEGylation . - pubmed
Herein we investigated the efficiency of PEGylation ( attachment of poly ( ethylene glycol ) ) to prevent benzyl alcohol - induced aggregation of the model protein alpha - chymotrypsinogen A ( aCTgn ) . - pubmed
KEY FINDINGS : When the model protein was reconstituted in buffer containing 0.9 % benzyl alcohol , copious amounts of buffer - insoluble aggregates formed within 24 h ( & gt;10 % ) . - pubmed
Benzyl alcohol - induced aggregation was completely prevented when two or five molecules of PEG with a molecular weight of 5000 Da were attached to the protein , whereas two or four molecules of bound 700 Da PEG were completely inefficient in preventing aggregation . - pubmed
Involvement of visfatin in palmitate - induced upregulation of inflammatory cytokines in hepatocytes . - pubmed
Visfatin - specific small interfering RNA significantly decreased the palmitate - induced mRNA expression and protein synthesis of interleukin-6 and tumor necrosis factor - alpha . - pubmed
Finally , by using the immunoabsorption method , binding partner proteins of two proteins residing in the interiors , PSD-95 and alpha - tubulin , and those of two proteins residing in the peripheral regions , elongation factor-1alpha and calcium , calmodulin - dependent protein kinase II alpha subunit , of cerebral and cerebellar PSDs were identified . - pubmed
A retrospective chart review was conducted on all patients treated with warfarin for a minimum of 14 days within a Long - Term Care ( LTC ) facility to compare Time within Therapeutic Range ( TTR ) between staff treated patients versus pharmacist treated patients . - pubmed
Design and synthesis of 8 - substituted benzamido - phenylxanthine derivatives as MAO - B inhibitors . - pubmed
We designed and synthesized a class of 8 - substituted benzamido - phenylxanthine derivatives as MAO - B inhibitors . - pubmed
The laboratory investigations showed a significant drop in hemoglobin and increased serum bilirubin . - pubmed
We identified that osmotic stress induces the phosphorylation of Raptor at Ser-696 , Thr-706 , and Ser-863 using liquid chromatography - tandem mass spectrometry . - pubmed
AP-3 binds to a PAR1 cytoplasmic tail - localized tyrosine - based motif and mediates PAR1 lysosomal degradation independent of ubiquitination . - pubmed
The ( I ) layers in the sesqui - dioxane and sesqui - benzene solvates are isostructural and unrelated to those in the hemi - MEK , mono - benzene and mono - toluene solvates . - pubmed
Rods of one kind are similar to analogous rods in the sesqui - dioxane and the sesqui - benzene solvates , whereas rods of the other kind are only remotely related to rods in the hemi - MEK solvate . - pubmed
The ability of IGFBP-3 to modulate the balance between pro - apoptotic and pro - survival sphingolipids by regulating sphingosine kinase 1 and sphingomyelinases may be integral to its role at the crossroads between cell death and survival in response to a variety of stimuli . - pubmed
Site-3 toxins bind primarily to the extracellular loops connecting transmembrane helical segments S1 - S2 and S3 - S4 in Domain 4 ( D4 ) and S5 - S6 in Domain 1 ( D1 ) and slow fast - inactivation of voltage - gated sodium channels . - pubmed
Scorpion alpha - toxin binding impedes D4S4 segment movement during inactivation since the modification rates of R3C in hNav1.4 with methanethiosulfonate ( CH3SO2SCH2CH2R , where R = - N ( CH3 ) 3 ( + ) trimethylammonium , MTSET ) and benzophenone-4 - carboxamidocysteine methanethiosulfonate ( BPMTS ) were slowed ~10 - fold in toxin - modified channels . - pubmed
The TARP gamma-8 isoform , which is highly expressed in the hippocampus , has a unique , long C - terminal domain with five distinct regions : two glycine - rich regions , a serine / arginine - rich region , a proline / alanine ( P / A ) rich region , and a PSD-95 / Dlg / ZO-1 ( PDZ ) binding motif . - pubmed
In addition , the GST - gamma-8 C - terminal tail ( residues 353 - 414 ) fusion protein containing the P / A - rich region bound to purified calcineurin in a Ca ( 2 + ) /calmodulin - dependent manner , whereas GST - gamma-8 with a deletion of the P / A - rich region did not . - pubmed
Our findings further suggest that the competition among various emission centers ( bright edge states ) and traps dominates the optical properties of these fluorescent carbon nanomaterials . - pubmed
Significance of osteopontin in the sensitivity of malignant pleural mesothelioma to pemetrexed . - pubmed
However , the mechanism of the sensitivity of MPM to PEM remains unclear . - pubmed
We analyzed the antitumor effects of PEM in six MPM cell lines by MTS assay . - pubmed
Pathway analysis revealed that osteopontin ( SPP1 / OPN ) was an important target in PEM sensitivity . - pubmed
PEM treatment with SPP1 / OPN knockdown inhibited the PEM - induced cell growth - inhibitory effect in PEM - sensitive cells . - pubmed
Expression of SPP1 / OPN and AKT phosphorylation significantly decreased after PEM treatment of the PEM - sensitive cells . - pubmed
PEM has antitumor effects in MPM cells dependent on SPP1 / OPN overexpression resulting in AKT activation . - pubmed
Our results suggest that SPP1 may be used as a single predictive biomarker of the effectiveness of PEM treatment in MPM . - pubmed
The voltage - gated sodium channel TPC1 confers endolysosomal excitability . - pubmed
The organelles have a previously uncharacterized , depolarization - activated , noninactivating Na (+ ) channel ( lysoNaV ) . - pubmed
Thus , TPC1 is a member of a new family of voltage - gated Na (+ ) channels that senses pH changes and confers electrical excitability to organelles . - pubmed
UNLABELLED : PURPOSES / AIM : Glucocorticoid steroids can induce expression of PPARgamma gene and enhance adipogenesis by bone marrow mesenchymal stem cells ( BMSCs ) , which may result in osteonecrosis of the femoral head . - pubmed
The purpose of this study was to evaluate the preventive effects of siRNA targeting PPARgamma gene on steroid - induced adipogenesis and osteonecrosis . - pubmed
The resulting mature form ( M(WT ) ) containing the catalytic domain and the PkdD showed optimum azocaseinolytic activity at 3 to 3.5 M NaCl , demonstrating salt - dependent stability . - pubmed
Activated platelets , in response to tissue damage , induce a proinflammatory program involving the aberrant expression of cyclooxygenase ( COX )- 2 , which leads to increased tissue concentrations of the proinflammatory and protumorigenic prostaglandin E2 . - pubmed
Low - dose aspirin acts as an antiplatelet agent by causing an irreversible inactivation of platelet COX-1 activity and the synthesis of thromboxane A2 . - pubmed
In this review we first discuss ion channels and transporters that function at the plasma membrane of melanocytes ; in the second part we consider ion transport across the membrane of intracellular organelles , with emphasis on melanosomes . - pubmed
CONCLUSIONS : Letrozole and everolimus synergistically inhibit the proliferation and induce cell apoptosis by inhibiting the cell signal pathway PI3K / Akt / mTOR and inducing cell apoptosis , thus showing a promising anti - tumor effectiveness . - pubmed
Reported here is the use of stable isotope labeling with amino acids in cell culture ( SILAC ) and pulse proteolysis ( PP ) for detection and quantitation of protein - ligand binding interactions on the proteomic scale . - pubmed
Increasing the substitute of carboxylic groups on CM - beta - CD was propitious to the formation of Ag nanoclusters and the stability of the produced silver nanoclusters was greatly improved . - pubmed
N - Acylethanolamine acid amidase ( NAAA ) is a cysteine amidase belonging to the N - terminal nucleophile ( Ntn ) hydrolases that primarily degrades anti - inflammatory and analgesic lipid amide palmitoylethanolamide . - pubmed
In this chapter , we review our contribution to ( i ) the determination of the reaction mechanism of amide hydrolysis catalyzed by cysteine Ntn - hydrolases and ( ii ) the discovery and optimization of active - site - directed inhibitors of NAAA characterized by a beta - lactone warhead . - pubmed
At H6 , parameters reflecting macrocirculation were not associated with mortality , whereas higher arterial lactate and mottling score were associated with death . - pubmed
The mean hemoglobin concentration was lower in women compared to men ( 11.7 vs. 12.6 g / dl , p & lt;= 0.0001 ) , while the blood urea and creatinine levels were higher in men ( p & lt ; 0.0001 ) . - pubmed
Erratum for : Eriocitrin and Apigenin as New Carbonic Anhydrase VA Inhibitors from a Virtual Screening of Calabrian Natural Products . - pubmed
In contrast to PFO- subjects , PFO+ subjects had 1 ) no improvement in A - aDO2 at rest and during exercise at ALT16 compared with ALT1 , 2 ) no significant increase in resting alveolar ventilation , or alveolar Po2 , at ALT16 compared with ALT1 , and consequently had 3 ) an increased arterial Pco2 and decreased arterial Po2 and arterial O2 saturation at rest at ALT16 . - pubmed
Furthermore , PFO+ subjects had an increased incidence of acute mountain sickness ( AMS ) at ALT1 concomitant with significantly lower peripheral O2 saturation ( SpO2 ) . - pubmed
Bromodeoxyuridine birth dating experiments in combination with immunohistochemical analysis using layer - specific markers showed that Dab2IP is important for proper positioning of a subset of layer II - IV neurons in the developing cortex . - pubmed
Here we present evidences of the effect of Thioflavin T ( ThT ) , a worldwide used fluorescent dye to monitor amyloid growth , on the Abeta ( 1 - 40 ) conformation , stability and aggregation . - pubmed
In particular ThT interactions with specific Abeta(1 - 40 ) residues promote a rigid partially - folded conformation which shifts the balance between different species in solution toward a more aggregation - prone ensemble of peptides , leading to aggregation . - pubmed
KIM-1 / TIM-1 is a phosphatidylserine receptor that is expressed on epithelial cells and can transform the cells into phagocytes . - pubmed
Desflurane preconditioning protects human umbilical vein endothelial cells against anoxia / reoxygenation by upregulating NLRP12 and inhibiting non - canonical nuclear factor - kappaB signaling . - pubmed
HUVECs were pre - conditioned with desflurane ( 1.0 MAC ) for 30 min , followed by a 15 - min washout , then exposed to 60 min anoxia and 60 min reoxygenation ( A / R ) , and incubated with 10 ng / ml tumor necrosis factor ( TNF )- alpha for 60 min . - pubmed
Desflurane preconditioning attenuated the effects of A / R and/or A / R plus TNF - alpha on cell viability , decreasing the levels of Smac and enhancing the levels of of cIAP1 ( P<0.05 ) . - pubmed
Preconditioning with desflurane also enhanced the mRNA levels of interleukin ( IL )- 10 and NLRP12 in the cells exposed to A / R by 2.40- and 2.16 - fold , respectively . - pubmed
Desflurane preconditioning enhanced HUVEC survival and protected the cells against A / R injury , and our results suggested that this process involved the upregulation of NLRP12 and the inhibition of non - canonical NF - kappaB signaling . - pubmed
This study aimed to establish the sequential static and static - dynamic supercritical carbon dioxide ( SDCO2 ) fractionation conditions to obtain a higher yield and desired chitosan with lower polydispersity index ( PDI ) and higher degree of deacetylation ( DD ) . - pubmed
Despite many studies on the effects of perinatal Bisphenol A ( BPA ) exposure on the brain , its effects on brain estrogen receptor ( ERalpha ) expression during puberty remain unclear . - pubmed
We used electrospray ionization - time of flight mass spectrometry to evaluate the degree of posttranslational modification of the entire HSA molecule that was associated with disease development and found that the fraction of Cys34 - cysteinylated HSA ( Cys - Cys34 - HSA ) , a major form of oxidative modification , varied substantially among the albumin preparations . - pubmed
These findings suggest that the Cys - Cys34 - HSA fraction , as estimated by electrospray ionization - time of flight mass spectrometry can be used as a predictive marker for the functional impairment of albumin preparations and that it would be preferable to use albumin preparations with higher contents of functionally effective albumin that correspond to a lower degree of cysteinylation of Cys34 in clinical practice . - pubmed
Pittsburgh sleep quality index ( PSQI ) , snore outcome survey ( SOS ) , epworth sleepiness score ( ESS ) , the lowest arterial oxygen saturation ( LSaO2 ) and AHI , time and ratio of slow wave sleep ( SWS ) stage and rapid eye movement ( REM ) stage were applied to comparing the curative effect between pre - operation and post - operation periods . - pubmed
BACKGROUND / AIM : There are controversial results regarding the relationship of serum 25 hydroxy vitamin D [ 25 ( OH ) D ] and ulcerative colitis ( UC ) activity . - pubmed
The alignment of SpA sequences enabled the clustering of several isoforms as a result of non - randomly distributed amino acid variations , located in two clusters of polymorphic sites in domains D to B and Xr ( a ) . - pubmed
In this report , the heterologous production of salicylate ( SA ) is the basis for metabolic extension to salicylate 2 - O - beta - d - glucoside ( SAG ) , a natural product implicated in plant - based defense mechanisms . - pubmed
When combined , SA and SAG production titers reached ~0.9g / L and ~2.5g / L , respectively . - pubmed
The SAG compound was then tested for anti - inflammatory properties relative to SA and acetylsalicylate ( aspirin ) . - pubmed
The risk for PD in these groups does not linearly increase with glucosylceramide ( GC ) accumulation or with acid beta - glucocerebrosidase ( GCase ) activity . - pubmed
Ambroxol hydrochloride , a pharmacological chaperone , which reduces endoplasmic reticulum ( ER ) stress induced by accumulation of mutant misfolded GCase could serve as such an agent . - pubmed
A3F and A3 G are deoxycytidine deaminases that inhibit HIV-1 replication by inducing guanine - to - adenine hypermutation through deamination of cytosine to form uracil in minus - strand DNA . - pubmed
Using size exclusion chromatography , rotational anisotropy , and in vitro deamination assays , we demonstrate that A3F promotes A3 G deamination activity by forming an A3F / G hetero - oligomer in the absence of RNA which is more efficient at deaminating cytosines . - pubmed
After APOBEC3 enzymes are encapsidated into virions , they deaminate cytosines in minus - strand DNA , which forms promutagenic uracils that induce transition mutations or proviral DNA degradation . - pubmed
plantarum ST8Sh to represent a potential probiotic candidate , including survival in the presence of low levels of pH and high levels of ox bile , production of beta - galactosidase , bile salt deconjugation , high level of hydrophobicity , functional auto- and co - aggregation properties , and adhesion to cell lines . - pubmed
Lowering cAMP in cystic tissues through the inhibition of the type-2 vasopressin receptor ( V2R ) constitutes a validated strategy to reduce disease progression . - pubmed
Mambaquaretin-1 is a full antagonist of the V2R activation pathways studied : cAMP production , beta - arrestin interaction , and MAP kinase activity . - pubmed
Mambaquaretin-1 interacts selectively with the V2R through its first loop , in the same manner that aprotinin inhibits trypsin . - pubmed
Mambaquaretin-1 represents a promising therapeutic agent against PKDs . - pubmed
This data demonstrates that beta1 integrin is required for the maintenance of glucose homeostasis through postnatal beta - cell function and expansion . - pubmed
Genuine splenic artery aneurysm rupture treated by N - butyl cyanoacrylate and metallic coils under resuscitative endovascular balloon occlusion of the aorta . - pubmed
We used 3.9x10 - 4 - 0.1 % HD , PD or DD , 3.9x10 - 4 - 0.0125 % PD , 7.8x10 - 5 - 0.02 % PHMB or CH concentration for 24 h to determine viability ( Cell Proliferation Kit XTT ) , proliferation ( Cell Proliferation ELISA BrdU kit ) , and migration using wound healing assay . - pubmed
HCEC proliferation AUC , comparing PD with PHMB , CH , DD , HD ( p & lt;= 0.016 ; PD worse for all ) and keratocyte proliferation AUC , comparing PHMB with HD , PD ( p = 0.004 ; p = 0.002 ; PHMB better for both ) , CH with HD , PD ( p & lt;= 0.001 ; CH better for both ) and DD with PD ( p = 0.043 ; DD better ) differed significantly . - pubmed
Keratocyte migration AUC comparing PD with control , PHMB , CH , DD and HD differed significantly ( p & lt;= 0.012 ; PD worse for all ) . - pubmed
In conclusion , our findings revealed that selective loss of neuronal AR actions impacts the kisspeptin / GnRH / LH cascade leading to compromised ovarian follicle dynamics . - pubmed
AIMS : This study describes differences in the partial pressures of exhaled nitric oxide ( PeNO ) between subjects fully acclimatized ( ACC ) to 5300 m and those who have just arrived to high altitude . - pubmed
In addition , systolic pulmonary artery pressure ( sPAP ) and arterial oxygen saturation ( SaO2 ) were measured in all participants . - pubmed
RESULTS : PeNO levels on day 1 were significantly higher in the non - ACC versus ACC cohort ( 8.7 + - 3.5 vs. 3.9 + - 2.2 nmHg , p = 0.004 ) . - pubmed
As the non - ACC group remained at altitude , PeNO levels fell and were not different when compared with those of the ACC group by day 9 ( 5.9 + - 2.4 vs. 3.9 + - 2.2 nmHg , p = 0.095 ) . - pubmed
TNF - alpha , interleukin-1 ( IL-1 ) , adiponectin ( ADP ) , nitric oxide ( NO ) and total nitric oxide synthase ( NOS ) levels were determined by enzyme linked immunosorbent assay ( ELISA ) , myeloperoxidase ( MPO ) activity was determined by colorimetric method , superoxide dismutase ( SOD ) activity was determined by hydroxylamine method , malondialdehyde ( MDA ) was determined by thiobarbituric acid colorimetric method , and catalase ( CAT ) activity was determined by UV spectrophotometry method . - pubmed
The inflammatory reaction in pioglitazone intervention groups was significantly reduced , TNF - alpha and IL-1 levels in serum and myocardium of DM+PGZ 4 mg and 8 mg groups were significantly decreased as compared with those of DM group [ serum : TNF - alpha ( ng / L ) was 268.33 + -46.57 , 261.34 + -33.73 vs. 331.40 + -69.05 , myocardium : TNF - alpha ( ng / L ) was 144.72 + -26.90 , 139.59 + -14.59 vs. 177.48 + -27.40 ; serum : IL-1 ( ng / L ) was 24.40 + -2.56 , 23.35 + -3.13 vs. 30.08 + -5.44 , myocardium : IL-1 ( ng / L ) was 21.26 + -2.85 , 20.54 + -2.75 vs. 24.78 + -3.60 , all P & lt ; 0.05 ] , and ADP levels were significantly increased [ serum ( mug / L ): 19.64 + -8.85 , 20.54 + -7.47 vs. 15.45 + -3.06 , myocardium ( mug / L ): 10.31 + -2.22 , 11.49 + -3.42 vs. 7.76 + -1.77 , all P & lt ; 0.05 ] , and the mRNA expressions of TNF - alpha and TLR4 in myocardium were significantly decreased ( TNF - alpha mRNA : 0.15 + -0.05 , 0.14 + -0.06 vs. 0.25 + -0.09 ; TLR4 mRNA : 0.57 + -0.17 , 0.40 + -0.18 vs. 0.75 + -0.35 , all P & lt ; 0.05 ) . - pubmed
Compared with the DM group , SOD and NO levels in serum and myocardium were significantly increased in DM+PGZ 4 mg and 8 mg groups [ serum : SOD ( U / L ) was 571.39 + -40.85 , 609.28 + -54.47 vs. 535.10 + -37.08 , myocardium : SOD ( U / mg ) was 55.74 + -8.12 , 53.60 + -9.87 vs. 42.26 + -12.34 ; serum : NO ( mumol / L ) was 2.95 + -0.51 , 2.99 + -0.43 vs. 2.03 + -0.78 , myocardium : NO ( nmol / mg ) was 1.95 + -0.37 , 2.11 + -0.26 vs. 1.56 + -0.33 , all P & lt ; 0.05 ] , the serum MDA levels were significantly decreased ( mumol / L : 20.11 + -2.34 , 19.70 + -2.02 vs. 23.07 + -3.06 , both P & lt ; 0.05 ) , while no significant effect on CAT . - pubmed
CONCLUSIONS : 4 mgxkg-1xd-1 pioglitazone could activate PPARgamma - TLR4 - TNF - alpha targeted pathway , thus inhibit inflammatory and oxidative stress factors expression , and down - regulate hyperglycemia induced myocardium inflammatory and oxidative stress level , but the effect did not show a dose dependent manner . - pubmed
RuBisCO large subunit gene primers for assessing the CO2 - assimilating planktonic community structure in Jiaozhou Bay , China . - pubmed
The protein coding genes ( rbcL / cbbL / cbbM ) for RuBisCO large subunit , the most abundant protein on earth that drives biological CO2 fixation , were considered as useful marker genes in characterizing CO2 - assimilating plankton . - pubmed
In this study , six different rbcL / cbbL / cbbM primers were employed to screen clone libraries to identify CO2 - assimilating plankton in Jiaozhou Bay . - pubmed
Bay-11 - 7082 also inhibited LPS - induced increases of ROS and Nrf2 expression . - pubmed
Background : Bevacizumab is a recombinant humanised monoclonal antibody to vascular endothelial growth factor shown to improve survival in advanced solid cancers . - pubmed
BRAF mutation positivity trended towards better OS with bevacizumab ( P = 0.21 ) . - pubmed
BRAF mutation status may predict for poorer OS untreated and potential benefit from bevacizumab . - pubmed
The review presents general principles for choosing optimal conditions for ecdysteroid separation , identification , and isolation using HPLC / TLC techniques in RP , NP - HILIC or NP modes . - pubmed
Thus , comprehensive overview of MS spectral parameters of ecdysteroids is provided . - pubmed
Furthermore , the first two genes of the Leloir pathway for d - galactose catabolism - encoding d - galactose 1 - epimerase and galactokinase - are induced more rapidly by l - arabinose than by d - galactose eventhough deletion mutants thereof grow as well as a wild type strain on the pentose . - pubmed
d - Galactose 1 - epimerase is hyperinduced by l - arabinose , d - xylose and l - arabitol but not by xylitol . - pubmed
nidulans , l - arabinose and d - xylose - both requiring NADPH for their catabolisation - actively promote the enzyme infrastructure necessary to convert beta - d - galactopyranose via the Leloir pathway with its alpha - anomer specific enzymes , into beta - d - glucose-6 - phosphate ( the starting substrate of the oxidative part of the pentose phosphate pathway ) even in the absence of d - galactose . - pubmed
Different SDs of APG were prepared by microwave , melted and kneaded technology using pluronic - F127 ( PL ) as a carrier . - pubmed
Significant enhancement in the dissolution rate and oral absorption of APG from SD suggested that developed SD systems can be successfully used for oral drug delivery system of APG . - pubmed
In this study , we obtained unprecedented AFM images of the Na , K - ATPase ( NKA ) pump after being reconstituted into DPPC and DPPC : DPPE liposomes . - pubmed
AFM data suggest that DPPC - NKA proteoliposomes are also rightside - out protein orientated , where the protrusions have an average height of 2.1 nm , while for DPPC : DPPE - NKA proteoliposomes , the majority of the protein reconstituted should be inside - out orientated , where the protrusions ' average height is 0.5 nm . - pubmed
Materials and Methods : Bacteria were cultured on a Yersinia Selective Agar medium ( " CIN - agar " ) at 28 C for 24 h. The microorganisms were grown in meat - peptone broth with 1.0 % glucose to measure the absolute values of the optical density of the culture . - pubmed
The specimens were examined with stereoscopic microscopy " BIOMED MS-1 Stereo " ( Russia ) and scanning electron microscope " TM 3030 plus " ( Holland ) . - pubmed
Factors Associated with the Need for Insulin as a Complementary Treatment to Metformin in Gestational Diabetes Mellitus . - pubmed
Those treated with metformin were compared with those who , in addition to metformin , also needed the combination with insulin . - pubmed
Of these , 366 ( 77.05 % ) were submitted to single therapy with metformin , and 109 ( 22.94 % ) needed insulin as a complementary treatment . - pubmed
CONCLUSION : Obesity resulted in an increased chance of the mother needing insulin as a complementary treatment to metformin , while FG & lt ; 90 mg / dL and primiparity were protective factors . - pubmed
A large number of randomized clinical trials and metanalyses provided strong evidence about therapeutic strategies acting on glucose metabolism such as GLP-1 RA and SGLT2i and about lipid - lowering treatment such as PCSK9i and Icosapent Ethyl . - pubmed
Interestingly , the associations between infant 's fasting glucose at age 6 and MYL6 , MYH11 , TNNT3 , TPM2 , CXCL2 , and NCAM1 were still relevant after correcting for multiple testing . - pubmed
At first , a three - step , one - pot procedure was developed to obtain reproducibly piperazine-2,6 - diones with various substituents at the N - atoms in high yields . - pubmed
Transformation of a piperazine-2,6 - dione with 1,4 - dibromobut-2 - ene and 3 - halo-2 - halomethylprop-1 - enes provided 3,8 - diazabicyclo [ 3.2.1 ] octane-2,4 - dione and 3,9 - diazabicyclo [ 3.3.1 ] nonane-2,4 - dione with a vinyl group at the C2- or a methylene group at the C3 - bridge , respectively . - pubmed
Since bridging via sulfinylimines and the one - pot bridging with 3 - bromo-2 - bromomethylprop-1 - ene gave promising yields , these strategies will be exploited for the synthesis of novel receptor ligands bearing various substituents in a defined orientation at the carbon bridge . - pubmed
Their metabolic profiles were studied via HPLC - PDA - ESI - MS / MS and afforded a tentative characterization of 98 compounds , including free organic acids , phenolic acid derivatives , flavonoid aglycones , flavonoid glycosides , flavonoids containing 3 - hydroxyl-3 - methylglutaroyl ( HMG ) units , coumarin derivatives and limonoids . - pubmed
Column chromatography resulted in isolation of six metabolites for the first time that were identified as nobiletin ( C1 ) , isosinensetin ( C2 ) , limonin ( C3 ) , 4 '- demethylnobiletin ( C4 ) , stigmasterol - O - glucoside ( C5 ) and hesperidin ( C6 ) . - pubmed
In vitro studies of the anti - inflammatory activity of DCM - L against cyclooxygenases ( COXs ) and 5 - lipoxygenase ( 5 - LOX ) enzymes revealed that DCM - L showed higher activity than the other tested fractions . - pubmed
Anti - inflammatory therapy with canakinumab targeting the IL-1beta innate immunity pathway could significantly reduce the rate of recurrent cardiovascular events than placebo . - pubmed
Given the well - documented role of mTOR in inhibiting autophagy , our results hence support the hypothesis that the antiaging mechanism of TPM - based medicines might involve autophagy induction . - pubmed
These antisera are capable of binding 125I - labeled tubulin in homologous or heterologous combinations , demonstrating immunological similarity between the tubulins . - pubmed
Differences between the tubulins were further resolved by radioimmunoassays , comparing the ability of each of the tubulins to inhibit the binding of each 125I - labeled tubulin to each antiserum . - pubmed
The effectiveness of insulin diminished as the concentration of these agonists increased , but insulin was able to suppress by 40 % the stimulation by a maximally effective concentration of epinephrine ( 1 muM ) . - pubmed
The response to glucagon , epinephrine , or insulin was not dependent upon protein synthesis as cycloheximide did not alter their effects . - pubmed
Insulin also suppressed the stimulation by isoproterenol of cyclic GMP . - pubmed
These data are the first demonstration of insulin antagonism to the stimulation of gluconeogenesis by catecholamines . - pubmed
Insulin reduced cyclic AMP levels which had been elevated by low concentrations of glucagon or by 1 muM epinephrine . - pubmed
This supports the hypothesis that the action of insulin to inhibit gluconeogenesis is mediated by the lowering of cyclic AMP levels . - pubmed
However , evidence is presented which indicates that insulin is able to suppress the stimulation of gluconeogenesis by glucagon or epinephrine under conditions where either the agonists or insulin had no measurable effect on cyclic AMP levels . - pubmed
Insulin reduced the glucagon stimulation of gluconeogenesis whether or not extracellular Ca2 + were present , even though insulin only lowered cyclic AMP levels in their presence . - pubmed
Insulin also reduced the stimulation by epinephrine plus propranolol where no significant changes in cyclic AMP were observed without or with insulin . - pubmed
In addition , insulin suppressed gluconeogenesis in cells that had been preincubated with epinephrine for 20 min , even though the cyclic AMP levels had returned to near basal values and were unaffected by insulin . - pubmed
Thus insulin may not need to lower cyclic AMP levels in order to suppress gluconeogenesis . - pubmed
Oral dexamethasone ( 8 - 12 mg ) administered over an 8 - 48 h period prior to delivery does not significantly alter placental ACTH content . - pubmed
These results indicate that the potencies of the TRH analogs in inducing shaking parallel their thyrotropin - releasing activities . - pubmed
This indicates dityrosine structures in the radiation - induced collagen aggregates . - pubmed
The data also suggest that the EGF / PDGF cascade branches into mitogenic and oncogenic signals , the latter of which is required for soft agar growth and focus formation . - pubmed
The technique was then used to screen Fab lots for batch - to - batch consistency , and for examining the effect of 10 mM cysteine on papain cleavage of whole antibody . - pubmed
Protein kinase C activators PMA 4 - phorbol 12 - myristate 13 - acetate and DiC8 ( 1,2 - dioctanoyl - sn - glycerol ) also increased arachidonic acid release , but the time course observed with PMA was different from that of IL-1 . - pubmed
To examine the role of other kinases in IL-1 stimulated arachidonic acid release , cultures were treated with H-7 ( 1 -( 5 - isoquinolinesulphonyl )- 2 - methylpiperazine dichloride ) , H-8 ( N -[ 2 -( methylamino ) ethyl ]- 5 - isoquinolinesulphonamide dichloride ) , HA1004 ( N -( 2 - guanidoinoethyl )- 5 - isoquinolinesulphonamide hydrochloride ) , and staurosporine . - pubmed
IL-1 stimulation of arachidonic acid release was blocked by H-7 , H-8 and staurosporine . - pubmed
Addition of H-7 at various times following IL-1 decreased IL-1 stimulated arachidonic acid release , suggesting that continued protein kinase activity was necessary for IL-1 action . - pubmed
Cycloheximide and actinomycin D inhibited the stimulation of arachidonic acid release by IL-1 , PMA or DiC8 . - pubmed
The addition of cycloheximide or actinomycin D 15 - 45 min after IL-1 also inhibited IL-1 stimulated arachidonic acid release , indicating that continued protein synthesis was required for IL-1 action . - pubmed
These clones became unresponsive to Ag after exposure to anti - CD3 . - pubmed
When compared with the effect of enflurane on transmural nerve stimulation - induced responses , exposure to enflurane resulted in slight attenuation of the contractile responses caused by tyramine , suggesting that enflurane may inhibit the responses to tyramine by interfering with an interaction between released norepinephrine and postjunctional alpha 1 - adrenoceptors rather than with tyramine - induced norepinephrine release . - pubmed
Addition of Ca2 + and Sr2 + to Ca2 + -depleted cells in the presence of BK produced a transient increase in Ca2 +i and Sr2 + i . - pubmed
Addition of Ba2 + to Ca2 + -depleted cells in the presence of BK produced an increase in Ba2 + i which continued to rise with time to a steady level . - pubmed
The antigen DR2 was more frequent in highly sensitized ( group C ) than in moderately sensitized ( group B ) patients with P = 0.002 , not significant after correction for the number of investigated antigens . - pubmed
Through a cannula inserted into a lateral cerebral ventricle a solution with a normal ( CSF , 152 mmol l-1 ) or high ( NaCSF , 1,000 mmol l-1 ) sodium ion content was infused . - pubmed
In vitro steroid sensitivity and interleukin 2 synthesis in normal subjects . - pubmed
Polymeric actin was localized in the reticulopodia by rhodamine - phalloidin staining . - pubmed
Simultaneous application of KCN and salicylhydroxamic acid ( an alternative oxidase inhibitor ) rapidly blocked all movement , indicating that motility is dependent on metabolic energy and that an alternative oxidative pathway functions in allogromiids . - pubmed
These changes were not accompanied by any changes in plasma levels of calcitonin or parathyroid hormone . - pubmed
The SH mRNA contains a single open reading frame which encodes a polypeptide of 44 amino acids with a molecular weight of 5,012 . - pubmed
[ Cobamide composition of aminopterin - resistant " folatereductase " ] . - pubmed
Rehydration as a diagnostic and therapeutic measure in hypercalcemia including an assessment of the calcium - lowering effect of porcine calcitonin . - pubmed
Effects other than the direct interaction of Cr ( III ) with DNA are inferred , which can cause infidelity of the DNA polymerase functions . - pubmed
After 7 days there was no significant difference between the efficacy of the two drugs with respect to ' pain at rest ' , ' pain on movement ' , ' tenderness ' or ' sleep disturbance ' but fenoprofen was significantly better when considering ' limitation of movement ' and ' The physician 's assessment of the severity of the condition ' . - pubmed
Ibuprofen did not significantly improve ' pain at rest ' but did improve the other parameters . - pubmed
Over a period of 3 to 12 months significant reductions in serum LH levels , LH / FSH ratios and in plasma testosterone were observed . - pubmed
and a 5 % alcohol solution during four weeks evoked strong increments of the serum enzyme activities of the aminotransferases ( GOT , GPT ) and the sorbitol dehydrogenase ( SDH ) . - pubmed
In plasma , concentrations of leucine , isoleucine , valine and proline were higher in Group II in the absence of glucose intake , than in the other groups . - pubmed
Diethylstilbestrol ( DES ) reduces significantly the plasma levels of testosterone , follicle - stimulating hormone ( FSH ) and luteinizing hormone ( LH ) . - pubmed
In contrast , those of prolactin , dehydoepiandrosterone ( DHEA ) , and cortisol as well as sexual hormone binding globulin ( SHBG ) are elevated . - pubmed
The additional administration of bromocriptine decreases prolactin to normal levels and further decreases the testosterone levels . - pubmed
The addition of cyproterone acetate to the therapeutic regimen decreases the cortisol levels , and all other values , including SHBG , are not altered significantly compared to the group treated with DES plus bromocriptine . - pubmed
Integrated regulation of very low density lipoprotein triglyceride and apolipoprotein - B kinetics in non - insulin - dependent diabetes mellitus . - pubmed
Turnover rates of plasma very low density lipoprotein ( VLDL ) triglyceride ( TG ) and apolipoprotein - B ( apo - B ) were significantly increased in age- and weight - matched groups of normolipemic and hyperlipemic mild diabetics , and hyperlipemic moderately severe diabetics when compared with normolipemic controls . - pubmed
The increase in LDL and/or VLDL remnant formation , regardless of prevailing plasma lipid levels or the severity of diabetes , provides a source of cholesterol which may account for the atherogeneity of this disorder . - pubmed
Increased hypoxia has been found after beta 2 adrenoceptor agonists ( but not adrenaline ) in asthmatics . - pubmed
Both the steady - state plasma and CSF concentrations of clonidine were proportional to the dose ; the ratio of CSF to plasma concentration was approximately 0.5 . - pubmed
The failure to see any change in CSF composition , significant spinal cord pathology , or signs of tissue or organ toxicity emphasizes the safety of epidurally administered clonidine at infusion rates up to 320 micrograms / hr and at infusate concentrations up to 2 mg / ml . - pubmed
In addition , direct binding of N - CAM to chondroitin sulfate could be demonstrated . - pubmed
Binding of N - CAM to the immobilized neurocan core protein could be inhibited with all recombinant fragments containing chondroitin sulfate or major parts of the mucin - like central region of neurocan . - pubmed
The activity of nucleotidyl transferase that adds CCA nucleotides to the 3 ' end of tRNA was also observed . - pubmed
Two fluorogenic probes anneal to the target between primers for c - erbB-2 and beta - globin genes and contain both a reporter dye ( 6 - carboxy - fluorescein ) and a quencher dye ( 6 - carboxy - tetramethyl - rhodamine ) . - pubmed
During the extension phase of the PCR cycle , the 5'-->3 ' exonuclease activity of Taq polymerase cleaves the hybridized fluorogenic probe , resulting in an increase of fluorescence emission of the reporter dye that is quantitative for the amount of PCR product and , under appropriate conditions , for the amount of template . - pubmed
Transformation studies showed that an aminoglycoside resistance gene , aadB , is carried on a 6.0 - kb plasmid ( pRAY ) in a clinical isolate of Acinetobacter ( strain SUN ) . - pubmed
Diversity of the E2P phosphoforms of Na , K - ATPase . - pubmed
Role of the carboxyl - terminal lectin domain in self - association of galectin-3 . - pubmed
Previous experimental results suggest that , when bound to multivalent glycoconjugates , galectin-3 self - associates through intermolecular interactions involving the amino - terminal domain . - pubmed
This mode of self - association is inhibitable by the lectin 's saccharide ligands . - pubmed
In addition , biotinylated galectin-3 bound to galectin-3 immobilized on plastic surfaces and the binding could also be inhibited by various saccharide ligands of the lectin . - pubmed
A mutant with a tryptophan to leucine replacement in the carboxyl - terminal domain , which exhibited diminished carbohydrate - binding activity , did not bind to galectin-3C - Sepharose 4B. - pubmed
Furthermore , galectin-3C formed covalent homodimers when it was treated with a chemical cross - linker and the dimer formation was completely inhibited by lactose . - pubmed
Therefore , galectin-3 can self - associate through intermolecular interactions involving both the amino- and the carboxyl - terminal domains and the relative contribution of each depends on whether the lectin is bound to its saccharide ligands . - pubmed
We found that the region between amino acid 353 and 393 is critical for the proliferation signal and activation of STAT6 , but not for tyrosine phosphorylation of Jaks . - pubmed
Gadolinium chloride ( GC ) , a rare earth lanthanide , has been used in in vivo studies to abrogate macrophage function to understand their role in tissue pathology . - pubmed
Although alkaline agarose electrophoresis of specific SV40 fragments showed that oxaliplatin formed interstrand cross - links , the levels of this lesion type were low . - pubmed
Low chlorophyll affects light absorptance but is unlikely to change light partitioning between photosystems . - pubmed
Both the minor ( GH I ) and the major ( GH II ) forms of purified GH appeared as single bands ( M(r ) approximately 23,000 ) after sodium dodecyl sulfate - polyacrylamide gel electrophoresis , as did the purified PRL ( M(r ) approximately 24,000 ) . - pubmed
Levodopa induces a cytoplasmic localization of D1 dopamine receptors in striatal neurons in Parkinson ' s disease . - pubmed
Mutations in the CNGB3 gene encoding the beta - subunit of the cone photoreceptor cGMP - gated channel are responsible for achromatopsia ( ACHM3 ) linked to chromosome 8q21 . - pubmed
Analysis of the extracts by gas chromatography - electron capture detector ( GC - ECD ) indicated that the herbicide trifluralin ( 2,6 - dinitro - N , N - dipropyl-4 - trifluoromethylaniline ) could be quantitatively extracted by using the SFE system proposed with small amounts of sample . - pubmed
After extraction of both lipoprotein ( a ) and low - density lipoprotein with glycerol prior to rotary shadowing , the protein components were observed to consist of a ring of density made up of nodules of different sizes , with apolipoprotein ( a ) and apolipoprotein B-100 closely associated with each other . - pubmed
However , when lipoprotein ( a ) was treated with a lysine analogue , 6 - aminohexanoic acid , much of the apolipoprotein ( a ) separated from the apolipoprotein B-100 . - pubmed
In 6 - aminohexanoic acid - treated preparations without glycerol extraction , lipoprotein ( a ) particles had an irregular mass of density around the core . - pubmed
In contrast , lipoprotein ( a ) particles treated with 6 - aminohexanoic acid in the presence of glycerol had a long tail , in which individual kringles could be distinguished , extending from the ring of apolipoprotein B-100 . - pubmed
As retinoids have been shown to inhibit the growth of breast cancer cells , loss of the proposed SEC14L1 retinal - binding function may contribute to breast tumorigenesis . - pubmed
Recessively inherited mutations in the HFE gene are responsible for iron accumulation in most cases of hereditary haemochromatosis and may have a role in HCV infection . - pubmed
AIMS : To assess the prevalence of HFE mutations in Scottish HCV infected patients and to explore the effect of the carrier state on serum and liver iron stores , and the severity of liver disease . - pubmed
Iron markers were assessed in serum ( ferritin , transferrin saturation ) and on liver biopsy ( stainable iron , liver iron concentration ( LIC ) and hepatic iron index ) . - pubmed
Carriage of HFE mutations was not associated with any clinical , biochemical , virological , or pathological features , including accumulation of liver iron . - pubmed
Carriage of HFE mutations , although frequently observed in these HCV infected patients , does not have a role in the accumulation of iron or the progression of liver disease in HCV infection . - pubmed
The BLM gene has 4437 bp and encodes 1417 amino acids . - pubmed
Apoptotic and necrotic cells were determined using Annexin - V and propidium - iodide staining . - pubmed
To identify inhibitors of the intrinsic N - acetylated alpha - linked acidic dipeptidase ( NAALADase ) activity of prostate specific membrane antigen ( PSMA ) that may be useful for targeting imaging agents or chemotherapeutic drugs to disseminated prostate cancer , analogs of the tetrahedral transition state for hydrolysis of the natural substrate , N - acetylaspartylglutamate ( NAAG ) , were synthesized . - pubmed
We have identified nine compounds , including fluorescent and iodine - labeled conjugates , which inhibit NAALADase enzyme activity with IC(50)s at , or below , 120nM. - pubmed
[ Dehydrogenases of higher fatty acids in the isolated liver in normal and pancreatectomized animals ] . - pubmed
The initial step in the formation of these diacids , which is thought to be rate limiting , is omega - hydroxylation by a cytochrome P450 ( CYP ) monooxygenase . - pubmed
tropicalis ATCC 20336 contains a family of CYP genes , and when ATCC 20336 or its derivatives are exposed to oleic acid ( C(18:1 ) ) , two cytochrome P450s , CYP52A13 and CYP52A17 , are consistently strongly induced ( Craft et al . - pubmed
CYP52A13 preferentially oxidized oleic acid and other unsaturated acids to omega - hydroxy acids . - pubmed
CYP52A17 also oxidized oleic acid efficiently but converted shorter , saturated fatty acids such as myristic acid ( C(14:0 ) ) much more effectively . - pubmed
Both enzymes , in particular CYP52A17 , also oxidized omega - hydroxy fatty acids , ultimately generating the alpha , omega - diacid . - pubmed
Consideration of these different specificities and selectivities will help determine which enzymes to amplify in strains blocked for beta - oxidation to enhance the production of dicarboxylic acids . - pubmed
Proliferation and osteogenic differentiation were analyzed by cell counting , an MTT assay , alkaline phosphatase activity , and total protein content . - pubmed
CD14 - positive cells cultured in the presence of PSK significantly increased the expression of HLA class II antigen and CD40 ; significantly increased the number and expression of CD80- , CD86- and CD83 - positive cells ; decreased Fluorescein isothiocyanate ( FITC)-dextran uptake , augmented IL-12 production ; augmented the allogeneic mixed lymphocyte reaction ; and induced antigen - specific cytotoxicity . - pubmed
We examined the SOD1 to GPX1 mRNA ratio in individual organs , as both enzymes form part of the body 's defense against oxidative stress , and because a disproportionate increase of SOD1 to GPX1 results in noxious hydroxyl radical damage . - pubmed
Comparative molecular field analysis and comparative molecular similarity indices analysis were performed on twenty five analogues of pimarane COX-2 inhibitor to optimize their cyclooxygenase-2 ( COX-2 ) selective anti - inflammatory activities . - pubmed
PE - induced phosphorylation of CREB was partially inhibited by Ro318220 and H89 , which were shown to be potent inhibitors of mitogen - and stress - activated protein kinase-1 ( MSK1 ) activation , implicating the involvement of this kinase in the response . - pubmed
In addition , inhibition of protein kinase A by RpcAMP reduced phosphorylation of CREB , suggesting that this pathway is also involved . - pubmed
An enhanced CBP / phospho - CREB complex formation was also observed , suggesting recruitment of CBP to phosphorylated CREB . - pubmed
BACKGROUND : HIV protease inhibitor ritonavir ( RTV ) is associated with many cardiovascular complications and causes vascular dysfunction through oxidative stress . - pubmed
RTV - treated arteries showed substantial reductions of eNOS mRNA by 77 % , eNOS protein by 72 % , and nitric oxide release by 37 % as compared with controls . - pubmed
The RTV plus curcumin - treated vessels showed substantial increases of eNOS and nitric oxide levels as compared with the RTV - treated vessels . - pubmed
Palladium - catalyzed one - pot synthesis of highly substituted furans by a three - component annulation reaction . - pubmed
Mechanism and influence factors of resonance light scattering ( RLS ) spectra of 4 -( 2 - pyridylazo )- resorcinol ( PAR ) aqueous solution were studied . - pubmed
The spectral feature of RLS is related to the absorption spectra of PAR . - pubmed
By a light polarization experiment , the RLS of PAR was demonstrated to be complete polarized light . - pubmed
Plasma RBP4 concentrations were found to be associated with sex , waist circumference , fasting plasma glucose , and insulin resistance . - pubmed
Of these , sex and fasting plasma glucose levels were found to be independent determinants of plasma RBP4 concentration . - pubmed
Serum samples were analyzed for adiponectin , inflammatory markers , and high density lipoprotein cholesterol ( HDL ) . - pubmed
RESULTS : Lipoatrophy was associated with higher triglycerides ( 330 vs 133 mg / dl , p = 0.0003 ) , lower HDL ( 33 vs 48 mg / dl , p = 0.02 ) , and a greater frequency of hypercholesterolemia ( total cholesterol & gt ; 200 mg / dl ; 64 % vs 23 % , p & lt ; 0.03 ) . - pubmed
Recent advances in this area indicate that glycosyltransferases involved in the biosynthesis of natural products have substrate flexibility regarding the sugar donor but also , less frequently , with respect to the aglycon acceptor . - pubmed
BACKGROUND : Real - time 3 - dimensional echocardiography ( RT3DE ) to visualize the coronary sinus ( CS ) was compared with multislice cardiac tomography ( MSCT ) . - pubmed
To assess the influence of temperature on calcium - triggered exocytosis , patch - clamp recordings of voltage - gated L - type Ca ( 2 + ) influx and exocytic membrane capacitance changes were performed at room ( 25 degrees C ) and physiological ( 35 - 37 degrees C ) temperatures . - pubmed
Immunohistochemistry of 1st and 3rd trimester placenta detected organic anion transporter 4 ( OAT4 ) and organic anion transporting polypeptide 2B1 ( OATP2B1 , former name OATP - B ) in cytotrophoblast membranes and at the basal surface of the syncytiotrophoblast , indicating that both transporter polypeptides are involved in placental uptake of foetal derived steroid sulfates . - pubmed
In contrast , DHEA - S uptake was only highly increased in OAT4 ( 40 times ) , but only weakly enhanced in OATP2B1 cells . - pubmed
The uptake of DHEA - S and E ( 1 ) S by OAT4 was partly Na(+)-dependent ( about 50 % ) , whereas uptake of DHEA - S by OATP2B1 was Na(+)-independent . - pubmed
In contrast , the affinity of DHEA - S towards OATP2B1 was about 10 times lower ( K(m)>200microM ) then for OAT4 ( K(m)=29microM ) . - pubmed
OATP2B1 seems not to be involved in de novo synthesis of placental estrogens but may contribute to the clearance of estrogen sulfates from foetal circulation . - pubmed
Pharmacological subversion of the S1P rheostat is proving to be clinically efficacious in multiple sclerosis , and both the scope and limitations of therapeutic modulation of the S1P axis in immunotherapy are becoming clearer as understanding of the integrated chemical physiology of the S1P system emerges . - pubmed
Influence of solid - state acidity on the decomposition of sucrose in amorphous systems II ( effect of buffer ) . - pubmed
blazei intake did not suppress ACF development or crypt multiplicity induced by DMH . - pubmed
Because benzodiazepines and ethanol have similar effect signatures , it has been hypothesized that these drugs share the gamma2 - containing GABA ( A ) receptors as a mechanism of action . - pubmed
While baseline learning and memory were unaffected by reduction of gamma2 containing GABA ( A ) receptors , ethanol dose - dependently impaired spatial memory equally in gamma2 heterozygous knockouts and wild type littermate controls . - pubmed
For instance , acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation , NGAL has a clear pro - tumoral effect , as has already been observed in different tumors ( e.g. - pubmed
Apolipoprotein E - containing lipoproteins ( LpE ) are generated in the central nervous system by glial cells , primarily astrocytes , and are recognized as key players in lipid metabolism and transport in the brain . - pubmed
Interestingly , a number of studies have also demonstrated that manipulating GR function with both agonists and antagonists has an antidepressant effect , and indeed that other drugs targeting the HPA axis and cortisol secretion - even drugs with opposite effects on the HPA axis - have antidepressant effects . - pubmed
Nicotine 's effect on hyperexcitability of inflamed neurons was blocked in the presence of an alpha ( 7 )- nicotinic acetylcholine receptor ( nAChR ) antagonist , methyllicaconitine , while choline , the alpha ( 7 )- nAChR agonist , induced a similar effect to that of nicotine . - pubmed
An almost fourfold increase in shelf life ( ~196 h ) was observed at the GA - predicted optimum ( protein concentration : 6.49 mg / ml , storage temperature : 20.8 C , Glycerol : 10.02 % , NaCl : 51.65 mM and pH : 8.2 ) . - pubmed
Changes in hormones , including testosterone , estrogen , cortisol , vasopressin , and prolactin , during the postpartum period in fathers may be biological risk factors in paternal PPD . - pubmed
Langmuir - Blodgett films were prepared by transferring the monolayers onto silica substrates and were subsequently analyzed by atomic force microscopy ( AFM ) . - pubmed
Here , through in situ high - resolution transmission electron microscopy ( HRTEM ) observations , we show that ( 1 ) in sharp contrast to what happens in bulk materials , in which plasticity is mediated by dislocation emission from Frank - Read sources and multiplication , partial dislocations emitted from free surfaces dominate the deformation of gold ( Au ) nanocrystals ; ( 2 ) the crystallographic orientation ( Schmid factor ) is not the only factor in determining the deformation mechanism of nanometre - sized Au ; and ( 3 ) the Au nanocrystal exhibits a phase transformation from a face - centered cubic to a body - centered tetragonal structure after failure . - pubmed
The Endo - F2 and Endo - F3 were efficient for transferring both sialylated and asia - lylated glycans and were highly specific for an alpha-1,6 - fucosylated GlcNAc - peptide as acceptor for transglycosylation , showing only marginal activity with non - fucosylated GlcNAc - peptides . - pubmed
In contrast , we found that the commonly used endoglycosidases such as Endo - A and Endo - M , which belong to GH family 85 , were unable to take alpha-1,6 - fucosyl - GlcNAc derivative as acceptors for transglycosylation . - pubmed
The novel activity of Endo - F2 and Endo - F3 was successfully applied for a highly convergent chemoenzymatic synthesis of a full - sized CD52 glycopeptide antigen carrying both terminal sialic acid and core fucose . - pubmed
This is the first report on endoglycosidases that are able to glycosylate alpha-1,6 - fucosylated GlcNAc derivatives to form natural core - fucosylated glycopeptides . - pubmed
The roles of their many potential receptors and/or binding proteins have been more difficult to assess at the genetic level due to the complexity of membrane interactions with these large proteins , but roles for MuSK - LRP4 in agrin signaling and for integrins , dystroglycan , and voltage - gated calcium channels in laminin - dependent phenotypes have been identified . - pubmed
The C allele rs2470152 was significantly associated with increased risk of vertebral fractures ( P = 0.04 ) , whereas none of the CYP19 polymorphisms showed differences in sex steroid levels between subjects with and without fractures . - pubmed
Platelet adenosine diphosphate receptor antagonists : ticlopidine to ticagrelor - a long continuing journey . - pubmed
Platelet adenosine diphosphate ( ADP ) receptor antagonists ( ticlopidine , clopidogrel , prasugrel , and ticagrelor ) are a major advance in the treatment of atherothrombotic diseases , especially acute coronary syndromes ( ACS ) . - pubmed
Ticagrelor is the latest non - thienopyridine ADP receptor blocker that is potent , effective , reversible , and relatively safer as compared to clopidogrel . - pubmed
We have earlier shown that stimulation of endothelin B receptors by IRL-1620 provides significant neuroprotection at 24h following cerebral ischemia . - pubmed
This study was designed to determine the effect of IRL-1620 on neurological functions , infarct volume , oxidative stress , and endothelin receptors following permanent middle cerebral artery occlusion for 7 days . - pubmed
All effects of IRL-1620 were blocked by endothelin B receptor antagonist , BQ788 . - pubmed
By promoting cellular cholesterol efflux in a cell autonomous manner and through its ability to enhance HDL function in hyperlipidemic plasma , apoE is now known to suppress atherosclerosis by controlling myeloid cell proliferation , monocyte activation and their capacity to infiltrate the vascular wall . - pubmed
Curcumin neither suppressed p53 mRNA transcription nor protected tumor cells against cisplatin cytotoxicity . - pubmed
Matrix metalloproteinase-1 ( MMP-1 ) is a member of the family of zinc - dependent endopeptidases that are capable of degrading extracellular matrix ( ECM ) and certain non - matrix proteins . - pubmed
The aim of this study was to compare the incidence of BMs in the pre- and post - docetaxel era in a single institution in which all oncological patients are referred to one Radiotherapy and one Medical Oncology Department . - pubmed
The year of the introduction of docetaxel into clinical practice ( 2002 ) divided the observation period into two 9 - year periods : period 1 ( P1 ) from 1994 until 2002 ( P1 ) , and period 2 ( P2 ) after 2002 . - pubmed
The plasma DOX measurements were performed by high - performance liquid chromatography with fluorescence detection , and the CKMB levels were assayed using the CKMB Labtest kit . - pubmed
Serum CKMB activity increased after conventional DOX administration but was unchanged in the DOX - ME group . - pubmed
Upon addition of Pb ( 2 + ) , it could specifically bind to its aptamer to form Pb ( 2 +)-stablized G - quadruplex and induce the aptamer strand to release from the electrode surface into solution , accompanied by the release of intercalated MB responsible for significant signal reduction . - pubmed
Between - group analysis did not reveal any differences except in the ratio of malondialdehyde ( MDA)-LDL over apolipoprotein B-100 ( MDA - LDL / apoB ) in pitavastatin vs. atorvastatin group ( -13 % vs. -0.7 % , p = 0.04 ) . - pubmed
Within - group changes from baseline to 12 - week revealed significant increases in OxPL / apoB and reductions in small - dense LDL , MDA - LDL , and lipoprotein - associated phospholipase A ( 2 ) measured on circulating apoB particles ( Lp - PLA(2)/apoB ) in both groups and significant reductions in OxPL / apoAI in the atorvastatin group . - pubmed
The determination of ETU and PTU was performed by high performance liquid chromatography with diode array detection ( HPLC / DAD ) or by gas chromatography with mass spectrometry detection ( GC / MS ) . - pubmed
The vls system alters the sequence of the expressed antigen by recombining gene fragments from unexpressed but divergent ' cassettes ' into the expression site , vlsE. - pubmed
Using the Ion Torrent PGM sequencer , we have analyzed a cohort of 297 unrelated Omani cases and reliably identified mutations in the beta - globin ( HBB ) gene . - pubmed
Nicotine exposure increases the complexity of dopamine neurons in the parainterfascicular nucleus ( PIF ) sub - region of VTA . - pubmed
BACKGROUND : Recent publications highlight differences within the sub - regions of the ventral tegmental area ( VTA ) including the parabrachial pigmented nucleus ( PBP ) , parainterfascicular nucleus ( PIF ) and paranigral nucleus ( PN ) in the projections to the prefrontal cortex ( PFC ) and the glutamatergic pathway . - pubmed
RESULTS : Our results have shown that the local field potentials corresponding to the neurons located in the PIF region of the VTA have ApEn values significantly higher ( p = 2x10 - 4 ) in the maternal nicotine cases when compared to the saline . - pubmed
CONCLUSION : Therefore , we speculate that the dopamine neurons located in the PIF sub - region of the VTA are very likely involved with the nicotine addiction . - pubmed
INTRODUCTION : Extracellular adenosine triphosphate ( ATP ) stimulates vasodilation by binding to endothelial ATP - selective P2Y2 receptors ; a phenomenon , which is posited to be accelerated during exercise . - pubmed
CONCLUSIONS : Oral ATP administration can increase post - exercise blood flow , and may be particularly effective during exercise recovery . - pubmed
The method was further used to assess the kinetics of hyperpolarized pyruvate - lactate exchange acquired in vitro in whole blood and to re - analyze the previously published in vitro reaction of hyperpolarized ( 15 ) N choline with choline kinase . - pubmed
Secondary outcomes included admission and duration of stay in the intensive care unit ( ICU ) , ventilation assistance , time to short - acting beta2 - agonists spaced at four hours or longer , supplemental asthma therapy , duration of supplemental oxygen , change from baseline in asthma severity , relapse after discharge , adverse health effects and withdrawals . - pubmed
Here we identify USP7 as a novel interacting protein of the oligonucleotide / oligosaccharide - binding fold of TPP1 , which was previously known to recruit telomerase to telomeres . - pubmed
We identify amino acids in TPP1 and USP7 that are critical for their interaction and multiple lysines within TPP1 that are oligo - ubiquitinated and deubiquitinated by USP7 . - pubmed
Lectins are proteins able to recognize carbohydrates , without modifying their structure , via the carbohydrate - recognition domain ( CRD ) . - pubmed
Endothelium - derived relaxant factors , extracellular calcium ( Ca ( 2 +) e ) and muscarinic receptors were also evaluated as putative participants in the CRLI relaxant effect . - pubmed
CRLI relaxant effect was blocked by L - NAME , a nonselective inhibitor of nitric oxide synthase ( NOS ) , and partially inhibited in a calcium - free solution ( 0Ca ) and by atropine , but it remained unchanged in the presence of indomethacin and TEA . - pubmed
In summary , our data suggest interaction between CRLI and muscarinic receptors located in vascular endothelial cells leading to NOS activation triggered by a mechanism that involves Ca ( 2 +) e along with the ability of CRLI to interact with heparan sulfate , a highly rated mechanoreceptor involved in eNOS activation . - pubmed
Films prepared from CNF processed after pretreatment with urea - containing microemulsions presented a more uniform fiber network and produced films with smoother surfaces compared to those based on ethylenediamine . - pubmed
This effect was inhibited either by antagonists of alpha7 - nAChRs , such as methyllycaconitine and alpha - cobratoxin , or by blocking SK - type calcium - activated potassium ( KCa ) channels with apamin or blocking intraterminal ryanodine receptors with ryanodine . - pubmed
First , the course of activation of the mTOR signaling pathway in PTZ - induced SE was examined to determine the time - point of peak activity , as reflected by phopshorylated ( p)-mTOR / mTOR and p - S6 / S6 ratios . - pubmed
Subsequently , pioglitazone was administrated intragastrically to investigate its effect on the mTOR signaling pathway , through western blot and immunochemical analyses . - pubmed
Compared with the vehicle treated - SE group , pretreatment with pioglitazone was associated with the loss of fewer neurons , lower levels of IL-1beta and IL-6 , and inhibition of the activation of the mTOR signaling pathway . - pubmed
Muscle glycogen , serum inflammatory cytokines , aspartic aminotransferase ( AST ) , alanine aminotransferase ( ALT ) , creatinine ( CRE ) , white blood cell ( WBC ) , neutrophil ( NEUT ) , red blood cell ( RBC ) , and hemoglobin ( HGB ) were measured . - pubmed
Consistent with the behavioral data , BST204 markedly increased muscle glycogen activity and concentrations of WBC , NEUT , RBC , and HGB . - pubmed
Also , tumor necrosis factor - alpha ( TNF - alpha ) and interleukin-6 ( IL-6 ) , AST , ALT , and CRE levels in the serum were significantly reduced in the BST204 - treated group compared to the control group . - pubmed
We designed an 18 - residue hybrid peptide ( PapMA ) incorporating N - terminal residues 1 - 8 of papiliocin and N - terminal residues 4 - 12 of magainin 2 , joined by a proline ( Pro ) hinge . - pubmed
Nuclear magnetic resonance experiments revealed that PapMA contained an N - terminal alpha - helix from Lys(3 ) to Lys(7 ) and a C - terminal alpha - helix from Lys(10 ) to Lys(17 ) , with a Pro(9 ) hinge between them . - pubmed
OBJECTIVE : In this study , nano - biocomposite composed of poly ( lactide - co - glycolide ) ( PLGA ) and chitosan ( CS ) were electrospun through a single nozzle by dispersing the CS nano - powders in PLGA solution . - pubmed
MATERIALS AND METHODS : In this experimental study , the PLGA / CS scaffolds were prepared at the different ratios of 90/10 , 80/20 , and 70/30 ( w / w ) % . - pubmed
RESULTS : H - ADSCs seeded on the matrices indicated that the PLGA / CS composite matrix with aligned nanofibres and higher content of CS nano - powders gave significantly better performance than others in terms of cell adhesion and proliferation rate ( P<0.05 ) . - pubmed
RESULTS : The results indicated that minocycline inhibited inflammation , decreased the expression of interleukin-1beta , tumor necrosis factor - alpha , and metalloproteinase - 9 , and squamous metaplasia features such as hyperproliferation and abnormal epidermal differentiation of conjunctival epithelium . - pubmed
Statistical analyses were performed using Plink and R. RESULTS : VKORC1 and CYP2C9 were the major genetic determinants of warfarin MWD and SMWD , with CYP4F2 having a smaller effect . - pubmed
Genotypes for VKORC1 and CYP2C9 * 3 , age , height and weight , as well as other clinical factors such as alcohol consumption , loading dose and concomitant drugs were important for the initial INR response to warfarin . - pubmed
VKORC1 and CYP2C9 genetic polymorphisms are the most important determinants of warfarin dosing , and it is highly unlikely that other common variants of clinical importance influencing warfarin dosage will be found . - pubmed
Both VKORC1 and CYP2C9 * 3 are important determinants of the initial INR response to warfarin . - pubmed
Cell blood count was assessed by sysmex system , serum iron and total iron binding capacity was measured by standard laboratory kit and for ferritin ELISA kit was utilized . - pubmed
NOX5 protein , one of the most active generators of reactive oxygen species ( ROS ) , plays an important role in many processes , including regulation of cell growth , death and differentiation . - pubmed
Contrary to other members of NADPH - oxidases family , NOX5 has its own regulatory calcium - binding domain and thus could be activated directly by calcium ions . - pubmed
In the present study using calorimetric methods and circular dichroism we found that oxidation of cysteine and methionine residues of NOX5 decreases binding of Ca2 + ions and perturbs both secondary and tertiary structure of protein . - pubmed
Our data strongly suggest that oxidation of calcium - binding domain of NOX5 could be implicated in its inactivation , serving as a possible defense mechanism against oxidative stress . - pubmed
Phosphatidic Acid Produced by RalA - activated PLD2 Stimulates Caveolae - mediated Endocytosis and Trafficking in Endothelial Cells . - pubmed
PLD2 was shown to be activated by RalA , and inhibition of PLD2 abolished Alexa-488 - BSA uptake , indicating that phosphatidic acid ( PA ) generated by PLD2 may facilitate caveolae - mediated endocytosis . - pubmed
Thus , our results suggest that the small GTPase RalA plays an important role in promoting invagination and trafficking of caveolae , not by potentiating the association between Cav-1 and FilA but by stimulating PLD2 - mediated generation of phosphatidic acid . - pubmed
Nodulation , nodule development and senescence directly affects nitrogen fixation efficiency , and previous studies have shown that inhibition of some cysteine proteases delay nodule senescence , so their nature inhibitors , cystatin genes , are very important in nodulation , nodule development , and senescence . - pubmed
On day three after seeding , the most apparent vinculin - containing focal adhesion plaques were also found on samples with higher concentrations of chromium . - pubmed
On the other hand , the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr - DLC samples with a lower concentration of Cr ( 0 - 1.8 at.% ) . - pubmed
The effects were assessed by comparing control and impact sites ( i ) on the composition of bacterial communities ; ( ii ) on the abundance of twelve antibiotic resistance genes ( ARGs ) encoding resistance to beta - lactams , fluoroquinolones , sulphonamides , tetracyclines , macrolides and vancomycin , as well as the class 1 integron - integrase gene ( intI1 ) ; ( iii ) on the occurrence of wastewater - associated bacteria , including putative pathogens , and their potential linkage to target ARGs . - pubmed
In this study , a DNA sample ( at 1 ng DNA target input in triplicate ) was spiked with five concentrations of five inhibitors ( humic acid , melanin , hematin , collagen , and calcium ) and sequenced with both the HID - Ion AmpliSeq Library Kit and ID panel on the Ion PGM System and the ForenSeq DNA Signature Prep Kit on the MiSeq FGx . - pubmed
The HID - Ion AmpliSeq Library Kit and ID panel and the ForenSeq DNA Signature Prep Kit both were susceptible to humic acid , melanin , and collagen ; however , the ForenSeq kit showed greater inhibition to melanin and collagen than the AmpliSeq kit . - pubmed
Because of reduced CF ASL volumes , CF mucus concentrations increased and produced a greater dependence of ASL volume regulation on cilia - mucus - ATP release interactions in CF than NL epithelial nodules . - pubmed
Similarly , the CF model was less tolerant to evaporation induced ASL volume reduction at all ATP release rates than the NL model . - pubmed
Previous studies indicate that genes GGT1 and HNF1A may contribute to the abnormal glucose metabolism and altered lipid profile that are important clinical features of PCOS . - pubmed
Our main outcome measures were the prevalence of major metabolic complications of acromegaly ( diabetes , hypertension , low - density lipoprotein cholesterol , triglycerides ) at diagnosis and last follow - up in comparison with French epidemiological data . - pubmed
Unlike FTR , FDR contains a rubredoxin [ 1Fe-0S ] center postulated to mediate electron transfer from ferredoxin to the active - site [ 4Fe-4S ] cluster . - pubmed
UV - visible , EPR , and Mossbauer spectroscopic data indicated that two - electron reduction of the active - site disulfide in FDR involves a one - electron - reduced [ 4Fe-4S]1 + intermediate previously hypothesized for FTR . - pubmed
Our results support a role for an active - site tyrosine in FDR that occupies the equivalent position of an essential histidine in the active site of FTR . - pubmed
At baseline and 4 h , middle cerebral artery blood flow velocity ( MCAv ) and CO2 reactivity ( CVR ) of the MCA and carotid artery were measured using transcranial Doppler ( TCD ) and duplex ultrasound , respectively . - pubmed
The groups were organized as Group I ( control ) , Group II ( malathion ) and Group III ( malathion + lipid emulsion treatment ) . - pubmed
RESULTS : A decrease of PAS positive staining cells , and an increase of liver enzymes , formation of degenerative changes and apoptotic cell deaths occurred in the malathion group . - pubmed
Within this context , high - rate activated sludge ( HRAS ) is the most promising alternative technology to redirect carbon ( organic compounds ) towards energy as biogas . - pubmed
However , the protective effects of Salvianic acid A and Danshen granule on Tat - induced neurotoxicity remain unknown . - pubmed
Here , we found that Salvianic acid A and Danshen granule remarkable inhibited Tat - induced cell death , blocked LDH release and rescued dendritic spine loss . - pubmed
Furthermore , Salvianic acid A and Danshen granule significantly ameliorates Tat - induced intracellular ROS and MDA production , attenuates cell apoptosis . - pubmed
In addition , Salvianic acid A and Danshen granule pretreatment obviously increases antioxidant enzymatic activity of CAT , SOD and GSH - Px and inhibits apoptotic pathways . - pubmed
In conclusion , this study demonstrated that Salvianic acid A and Danshen granule provides substantial neuroprotection against Tat - induced neurotoxicity , which may be new therapeutic agent in Tat induced HAND or neurodegenerative diseases . - pubmed
Fisetin decreased the expression of multiple signaling proteins ( beta - catenin , NF - kappaB , EGFR , STAT-3 ) acting upstream to EMT and known to be involved in induction and maintenance of mesenchymal phenotype , which may explain the observed effects . - pubmed
Overall , our study suggested the ability of fisetin to serve as a potential therapeutic agent on its capacity to attenuate the EMT program and inhibit migration , invasion and stem cell phenotype of lung cancer cells . - pubmed
To date , researchers have found that some chloroplast proteins have Ca2 + -binding sites , and the structure and function of some of these proteins have been discussed in detail . - pubmed
We have shown a role for ARNT / HIF1beta in glucose sensing and insulin secretion in vitro and no defects in in vivo glucose homeostasis . - pubmed
The effects of the HF diet rescued insulin secretion in fl / fl / Cre islets could be reproduced by treating low fat diet fed fl / fl / Cre islets with the lipid signalling molecule 1 - monoacylglcyerol . - pubmed
This suggests that the defects seen in low fat diet fed fl / fl / Cre islet insulin secretion involves lipid signalling molecules . - pubmed
A multifunctional nanoparticle was simply synthesized by mixing a TICT + AIE featured molecule ( TPAPP - CHO ) with PBS solution . - pubmed
[ IgA vasculitis with massive hemorrhage from the jejunum after steroid administration ] . - pubmed
Importantly , the diameters and surface charges of the FNPs changed dramatically after 2 h of post - treatment using 0.4 M hydrogen peroxide ( H2O2 ) as the oxidant , indicating that the FNPs were highly ROS - sensitive . - pubmed
In this Viewpoint , we review the state - of - the art of PET imaging with radiotracers that target the GABAA - benzodiazepine receptor complex , challenges and opportunities for imaging GABAB receptors and GABA transporters , and highlight an ongoing need to develop more sensitive radiotracers for imaging GABA release in the central nervous system . - pubmed
After 4 weeks of treatment , cinacalcet significantly decreased the oxidized albumin ratio which is a ratio of reduced and oxidized forms of HSA via increasing reduced form of HSA . - pubmed
In conclusion , cinacalcet improves the redox status of HSA by inhibiting PTH production and partially by its radical scavenging action . - pubmed
Overall , this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non - invasive PET / CT imaging to evaluate new therapies in the preclinical area . - pubmed
The proportion of statins , aspirin , clopidogrel , angiotensin - converting enzyme inhibitors / angiotensin II receptor blockers ( ACEI / ARB ) , and beta - blocker used at admission was 88.8 % , 86.9 % , 84.6 % , 51.7 % , and 61.4 % , respectively . - pubmed
fermentum 111 , to remove aflatoxin M1 ( AFM1 ) from a phosphate buffer solution ( PBS ; spiked with 0.15 microg / mL AFM1 ) . - pubmed
Levels of AFM1 in PBS were measured with high - performance liquid chromatography . - pubmed
Viable and non - viable cells of all examined isolates were capable of removing AFM1 in PBS with removal percentage values in the range of 73.9 - 80.0 % and 72.9 - 78.7 % , respectively . - pubmed
Since the historical use of gonadotrophin and estradiol levels to define the different anovulatory disorders has shown some limitations , the use of other markers such as anti - mullerian hormone ( AMH ) has been proposed . - pubmed
Effects of Sr2 + and Mg2 + on the phospholipase A and the presynaptic neuromuscular blocking actions of beta - bungarotoxin , crotoxin and taipoxin . - pubmed
The antitumor effects in vivo of rhIL-6 appear to be mediated indirectly as rhIL-6 had no effect on proliferation of LLC cells in vitro as assessed by colony and 3H - thymidine incorporation assays . - pubmed
Phorbol myristate acetate ( PMA ; 20 - 100 ng / ml ) triggered only acetyltransferase activation , without concomitant lyso - paf ( 1 - O - alkyl - sn - glycero-3 - phosphocholine ) and paf formation . - pubmed
A low concentration of PMA ( 5 ng / ml ) potentiated antigen - induced degranulation , acetyltransferase activation and paf formation by about 30 % but did not change the level of lyso - paf formation . - pubmed
Overnight treatment of mast cells with PMA ( 200 ng / ml ) caused disappearance of protein kinase C ( PKC ) from both cytosol and membranes . - pubmed
The PKC inhibitors chlorpromazine and staurosporine inhibited to a significant extent both cell degranulation and all steps leading to paf biosynthesis . - pubmed
The PKC - dependent signal directly generated by PMA or diacylglycerol is not sufficient to trigger the full cell response , which is obtained only through receptor - operated antigen challenge . - pubmed
Inhibition of cyclo - oxygenase with indomethacin , aspirin or meclofenamate abolished the vasoconstrictor effect of bradykinin , showing that a member of the arachidonic acid cascade may be involved . - pubmed
The endoperoxide H2 / thromboxane A2 receptor antagonist SQ 29548 significantly reduced the vasoconstrictor effect of bradykinin , but did not affect the vasoconstrictor response to noradrenaline , adrenaline , vasopressin , 5 - hydroxytryptamine or prostaglandins . - pubmed
We isolated and characterized a rat neutrophil defensin , RatNP-2 , that differs from the previously described defensin RatNP-1 by containing Ser-7 in place of Arg-7 . - pubmed
A study was made of the number of pulmonary endocrine cells , immunoreactive for gastrin - releasing peptide ( bombesin ) or calcitonin , in the terminal bronchioles of 39 cases of pulmonary vascular disease . - pubmed
Proliferating cells arrested at the G1 - S boundary by exposure to cytosine arabinoside showed an increased PCNA immunofluorescence as compared to unstimulated cells . - pubmed
However , the physiological phase consists of the activation of a KCl + NaCl carrier , while the Na + in turn is pumped out by Na +- K + ATPase , with a resultant net accumulation of KCl . - pubmed
, antigens seen in the context of self - class I molecules . - pubmed
[ Triglyceride and low - density lipoprotein cholesterol ( LDL - C ) levels in a population of men aged 35 - 54 years from 8 industrial plants in Wroclaw ] . - pubmed
Heptachlor and chlordane also caused only partial inhibition of oxidation , but were more toxic with cell - free extract nitrite oxidase . - pubmed
Differential response patterns to MAO inhibitors and tricyclics . - pubmed
We found a linear negative correlation ( P less than 0.001 ) between the amount of LDL cholesterol in the incubation solution and prostaglandin I2 synthetase activity , whereas there was a positive correlation ( P less than 0.01 ) between HDL cholesterol and prostaglandin I2 synthesis . - pubmed
A very low concentration of LDL cholesterol and a high concentration of HDL cholesterol stimulated prostaglandin I2 synthesis , whereas a high LDL cholesterol concentration inhibited prostaglandin I2 biosynthesis by 64 % . - pubmed
The concentration range of LDL and HDL cholesterol was representative of physiologically low , normal or elevated levels of lipoproteins . - pubmed
The examinations included measurements of erythrocyte count , hematocrit , mean corpuscular volume ( MCV ) , blood and urine lead concentrations , urine coproporphyrin and delta - aminolevulinic acid , membrane cholesterol ( CHO ) and phospholipids ( PL ) , total and free CHO in plasma , and lecithin cholesterol acyltransferase ( LCAT ) activity in plasma . - pubmed
But no significant difference was observed in total CHO in plasma , erythrocyte count , hematocrit , MCV , and LCAT activity between both groups . - pubmed
We proved that ( 1 ) elevated pulmonary arteriolar resistance ( PAR ) is not related to pulmonary blood flow and volume , pleural pressure , arterial oxygen or carbon dioxide tension and pH , left ventricular filling pressure and function ; ( 2 ) systemic vascular resistance ( SVR ) significantly correlates with PAR ; ( 3 ) calcium - channel blockade with nifedipine reduces systemic and pulmonary arterial pressures toward normal and significantly lowers both SVR and PAR ; ( 4 ) the percent decrease in vascular resistance after nifedipine is related to the baseline level of resistance in both the greater and the lesser circulations . - pubmed
Formic acid pretreatment markedly enhanced the reactivity to anti - IAPP8 - 17 antibody ; however , it failed to show the reactivity to anti - N - terminal and C - terminal flanking peptide antibodies . - pubmed
Formic acid pretreatment of pancreatic tissue sections prepared for immunostaining is useful for visualization of buried epitopes of mature IAPP and its precursor molecules , either in islet amyloid deposits or in the islet cells . - pubmed
At high loadings the specific activity of immobilized whole antibody was lower for the large antigen than for the small hapten , whereas no effect of hapten size on n was evident for either immobilized Fab ' or Fv fragments . - pubmed
There was a significant reduction in serum insulin levels and glucose clearance rates without overt diabetes for 30 Gy and 35 Gy ( p & lt ; 0.05 ) . - pubmed
The concentrations of VPA in plasma ( Css for total and C(uss ) for unbound drug ) and various tissues ( CT ) were determined . - pubmed
Nuclease stability as dominant factor in the antiviral activity of oligonucleotides directed against HSV-1 IE110 . - pubmed
A minimal protection strategy , consisting of end - capping and pyrimidine protection , has proven to be particularly useful , because it not only yields nuclease - resistant oligonucleotides but also minimizes non - sequence - specific effects . - pubmed
However , there was loss of circadian variation in serum cortisol concentrations and administration of high - dose oral dexamethasone was followed by a marked and reproducible rise in both ACTH and cortisol . - pubmed
A good histologic response , an LDH baseline value within the normal range , and the use of high - dose MTX were positive predictive factors for DFS . - pubmed
CONCLUSIONS : In non - metastatic osteosarcoma of the extremity , chemotherapy - induced tumor necrosis , the baseline LDH serum value and the use of HDMTX are significant predictive factors for DFS . - pubmed
Adaptation of gamma - aminobutyric acid type A receptors to alcohol exposure : studies with stably transfected cells . - pubmed
We studied the adaptation of gamma - aminobutyric acid type A ( GABAA ) receptor function to chronic ethanol exposure in cells stably transfected with the following GABAA receptor subunits : alpha-1 beta-2 gamma-2L , alpha-1 beta-2 gamma-2S , alpha-1 beta-3 gamma-2S , alpha-1 beta-1 , alpha-5 beta-3 gamma-3 and alpha-6 beta-3 gamma-2S. - pubmed
Direct activation of the GABAA receptor by pentobarbital was enhanced by chronic ethanol treatment . - pubmed
Chronic ethanol exposure produced a reduction in the Emax value for the action of muscimol for all six subunit combinations , but quantification of surface receptors by immunolabeling showed no change in GABAA receptor density . - pubmed
The differences in alcohol sensitivity and time courses for different effects of ethanol indicate multiple mechanisms of adaptation of GABAA receptors . - pubmed
Since glutamate reuptake is the primary mechanism for controlling extracellular glutamate levels , the present study was conducted to examine whether chronic neuroleptic exposure alters gene expression for the glutamate transporter GLT-1 in the striatum . - pubmed
Although both haloperidol and clozapine treatment for 30 days significantly decreased GLT-1 expression from normal , the effects of haloperidol treatment were consistently , and in the dorsal striatum , significantly greater than those of clozapine . - pubmed
When unfolded proteins accumulate in the endoplasmic reticulum ( ER ) , transcription of glucose - regulated proteins ( GRPs ) representing ER - resident molecular chaperones is markedly induced via the unfolded protein response ( UPR ) pathway . - pubmed
In a fourth , iron gluconate ( 62.5 mg i.v./HD ) increased transferrin saturation to 27 % and reduced the percentage of hypochromic red cells from 12 to 4 , while reticulocyte haemglobin remained & gt ; 30 pg . - pubmed
Transposon mini - Tn10 insertional mutagenesis was applied to strain 406 , which revealed that lpa-8 ( sfp ) ( encoding 4 '- phosphopantetheinyl transferase ) and the pps operon ( located between 167 and 171 degrees ) are essential for plipastatin production . - pubmed
By using a new high - performance liquid chromatography system , we revealed that strain 168 expressing only lpa-8 can also produce plipastatin , although the yield is very low . - pubmed
However , the introduction of the pleiotropic regulator degQ of strain YB8 into strain 168 expressing lpa-8 resulted in a 10 - fold increase in the production of plipastatin . - pubmed
Air entrainments on charcoal filters , extracted with CS ( 2 ) and subsequently with acetone , were analyzed by coupled gas chromatography ( GC)-electroantennogram ( EAG ) as well as by GC - mass spectrometry . - pubmed
The CS(2 ) extract contained 12 EAG - active peaks from which benzaldehyde , octanal , limonene , 1,8 - cineol , methyl benzoate , nonanal , decanal and geranyl acetone were identified . - pubmed
In the acetone extract we identified eight EAG - active peaks as phenol , nonanal , 2 - ethylhexanoic acid , octanoic acid , benzoic acid , nonanoic acid , decanoic acid and dimethyl phthalate . - pubmed
Two acidic amino acid residues , Asp(470 ) and Glu(471 ) , contained in the carboxyl cytoplasmic tail of a major lysosomal membrane protein , LGP85 / LIMP II , are important for its accumulation in secondary lysosomes . - pubmed
Lysosomal membrane glycoprotein termed LGP85 or LIMP II has a COOH - terminal cytoplasmic tail whose amino acid sequence is R(459)GQGSMDEGTADERAPLIRT(478 ) . - pubmed
Two acidic amino acid residues , D(470 ) and E(471 ) , in the cytoplasmic tail of LGP85 are crucial for its binding to adaptor - like complex AP-3 . - pubmed
In the present study we investigated their role(s ) in intracellular distributions of LGP85 using two alanine substitution mutants at D(470 ) and E(471 ) ( defined as D470A and E471A , respectively ) . - pubmed
Plasma fatty acids , blood lipids , and apolipoproteins were analyzed at 15 months , and dietary intakes at 12 , 15 , and 18 months . - pubmed
In vivo , the TPGS - incorporated film exhibited a two - fold increase in FA when compared to the control film containing the PEG 3350 5 % . - pubmed
The carbomer 971P and polycarbophil containing films were determined to have the highest FA and EAF , and the lowest MA of all films tested . - pubmed
ChAT activity was increased by retinoic acid , dexamethasone , and dibutyrylcyclic AMP ( dbcAMP ) , and a synergistic effect was observed with a combined dexamethasone and dbcAMP treatment . - pubmed
Hemicholinium-3 - sensitive choline uptake , hemicholinium-3 binding , and acetylcholine content were increased by differentiating agents , with a rank order of potency comparable to their effects on ChAT activity . - pubmed
Some of the carbohydrate components detected by ELLAs as well as two organic matrix proteins ( SM30 and SM50 ) were localized within different subregions of the spine skeleton using field - emission scanning electron microscopy . - pubmed
Lysine and polyamines are substrates for transglutamination of Rho by the Bordetella dermonecrotic toxin . - pubmed
Bordetella dermonecrotic toxin ( DNT ) catalyzes the transglutamination of glutamine-63/61 of Rho GTPases , thereby constitutively activating Rho proteins . - pubmed
The enzymatically active fragment of DNT ( residues 1136 to 1451 , DeltaDNT ) induced the incorporation of L -[( 14 ) C ] lysine in RhoA in a concentration - dependent manner . - pubmed
Also , Rac and Cdc42 , but not Ras , were transglutaminated with lysine by DeltaDNT . - pubmed
Transglutamination of the GTPase with L - lysine inhibited intrinsic and Rho - GAP - stimulated GTP hydrolysis of RhoA. - pubmed
In contrast to lysine , treatment of RhoA with alanine , arginine , and glutamine were not able to substitute for lysine in the transglutamination reaction . - pubmed
A comparison of putrescine , spermidine , and lysine as substrates for DNT - induced transglutamination of RhoA revealed that lysine is a preferred second substrate at least in vitro . - pubmed
Time - segmented MS2 scans enabled simultaneous monitoring of Met - enkephalin , Leu - enkephalin , and unknown peptides . - pubmed
Blood glucose monitoring is recommended : to evaluate the glycemic response to the effect of insulin ; to confirm hypoglycemia ; to prevent hyperglycemia in case of disease ; in case of physical exercise . - pubmed
Flavopiridol , a cyclin - dependent kinase inhibitor , enhances radiosensitivity of ovarian carcinoma cells . - pubmed
Flavopiridol , a cyclin - dependent kinase ( cdk ) inhibitor , can cause cell cycle arrest , induce apoptosis in cancer cells , and inhibit tumor cell growth in vivo . - pubmed
In addition , flavopiridol treatment ( 300 nM , 1 day ) resulted in decreased levels of Ku70 and Ku86 proteins that play a role in DNA repair processes , suggesting that DNA repair processes may have been disrupted by this agent . - pubmed
Flavopiridol down - regulated cyclin D1 and cyclin E protein levels and also inhibited phosphorylation of retinoblastoma protein , which is inconsistent with the observed cell cycle arrest . - pubmed
Among the cdks tested , cdk-9 , the catalytic subunit of positive transcription elongation factor b , was significantly down - regulated by flavopiridol , suggesting that flavopiridol may modulate cellular transcription processes . - pubmed
The objective of this study was to investigate the use of folate - targeted liposomes for the delivery of encapsulated oligonucleotides to folate receptor ( FR)-positive tumor cells in vitro and in vivo . - pubmed
Importantly , cell association was blocked by a large excess folic acid , thus reflecting the FR - specific nature of the cell interaction . - pubmed
We conclude from these results that folate liposomes can effectively deliver oligonucleotides into folate receptor - bearing cells in vitro , but additional barriers exist in vivo that prevent or decrease effective tumor uptake and retention . - pubmed
Ca ( 2 + ) sensitivity of arterial contractility is governed by regulating myosin phosphatase activity in response to agonist stimuli . - pubmed
Agonists , such as an alpha(1)-agonist and endothelin-1 , produced significant contraction in arteries of all species under physiological Ca ( 2 +)-containing conditions . - pubmed
Thus , CPI-17 is essential for G protein / PKC - mediated Ca ( 2 + ) sensitization in smooth muscle . - pubmed
The ability of Fe chelation by 100 microM SIH to limit enlargement to approximately 180 % confirms that the LIP plays a major role in cytomegaly . - pubmed
Pretreatment of the cells with PACAP resulted in a significant decrease in LPS- or IFNgamma - induced NO production as well as iNOS and IL-1beta mRNA levels . - pubmed
The inhibitory effect of PACAP appeared to be mediated through an increase in intracellular cAMP . - pubmed
PACAP inhibition of LPS - induced NO production was accompanied by inhibition of p38 MAPK activation , but not ERK , JNK , or NF - kappaB. - pubmed
Topotecan - induced alterations in the amount and stability of human DNA topoisomerase I in solid tumor cell lines . - pubmed
MATERIALS AND METHODS : Western blot analysis of the time - dependent redistribution of a full - size topo 1 and its proteolytical fragments was performed after Tpt treatment for 1 h at concentrations 10 - fold or 100 - fold higher than the Tpt IC50 for the respective cell lines . - pubmed
CONCLUSION : The data obtained indicate that Tpt - induced time- and dose - dependent effects on the amount and stability of topo 1 are involved in the mechanisms of Tpt activity against different solid tumor cell lines . - pubmed
This is in sharp contrast to the importance of this type of proteins , particularly G protein - coupled receptors ( GPCRs ) , as today 60 - 70 % of the current drug targets are based on membrane proteins . - pubmed
ATP hydrolysis in the presence and absence of ouabain was used to measure Na , K - ATPase activity . - pubmed
Western blot analysis was used to examine phosphotyrosine - containing proteins and tyrosine kinase expression . - pubmed
Na , K - ATPase activity in membrane material treated with Lck or Fes was not significantly altered , even though Lck and Fes treatment induced robust tyrosine phosphorylation . - pubmed
Added exogenously , each tyrosine kinase induced a different pattern of membrane protein tyrosine phosphorylation . - pubmed
As judged by immunoprecipitation , Src , Fyn , Lyn , and Lck elicited tyrosine phosphorylation of the Na , K - ATPase alpha1 protein . - pubmed
This could perhaps explain why inhibition of Na , K - ATPase activity is reported to be caused by tyrosine phosphorylation in some tissues , whereas stimulation of Na , K - ATPase activity is observed in other tissues . - pubmed
We investigated whether H2O2 , superoxide , and ERK participate in nerve growth factor ( NGF)-induced signaling cascades and whether antioxidant N - acetylcysteine ( NAC ) regulates these NGF - induced responses . - pubmed
We found that NGF increased intracellular H2O2 concentration and activated ERK but failed to affect intracellular superoxide level . - pubmed
Moreover , NAC counteracted these NGF - induced responses . - pubmed
The variability in CsA and Tac disposition has been attributed to interindividual differences in the expression of the metabolizing enzymes cytochrome P450 ( CYP ) 3A4 and 3A5 , and in the expression of the drug transporter P - glycoprotein ( encoded by the ABCB1 gene , formerly known as the multidrug resistance 1 gene ) . - pubmed
In addition , we determined if DCEs could serve as surrogate inducers of BMO gene expression . - pubmed
The impacts of lactic acid - supported DCE oxidation on BMO activity differed among the isomers . - pubmed
In intact cells , 50 % of BMO activity was irreversibly lost after consumption of approximately 20 nmol mg protein(-1 ) of 1,1 - DCE and 1,2 - trans DCE in 0.5 and 5 min , respectively . - pubmed
In contrast , a comparable loss of activity required the oxidation of 120 nmol 1,2 - cis DCE mg protein(-1 ) . - pubmed
butanovora " did not express BMO activity but gained activity after exposure to butane , ethene , 1,2 - cis DCE , or 1,2 - trans DCE . - pubmed
The products of BMO activity , ethene oxide and 1 - butanol , induced lacZ in a reporter strain containing lacZ fused to the BMO promoter , whereas butane , ethene , and 1,2 - cis DCE did not . - pubmed
1,2 - trans DCE was unique among the BMO substrates tested in its ability to induce lacZ expression . - pubmed
Pre - incubation with 50 microM 3 - quinuclidinyl benzilate ( QNB ) or 50 nM strychnine failed to inhibit the ACh - initiated AR , demonstrating that muscarinic AChRs and nAChRs containing alpha9 subunits do not mediate this event . - pubmed
Choline ( 2.5 , 5 and 10 mM ) , a highly specific but low potency agonist of the alpha7 nAChR initiated AR , with its effect blocked by the nAChR antagonist methyllycaconitine ( MLA ) . - pubmed
The nAChR antagonists , alpha - bungarotoxin ( alpha - BTX ) and MLA , reduced the ACh - initiated Ca2 + rise by 75 and 78 % , respectively , demonstrating the majority of the rise is mediated through nAChRs containing alpha7 or alpha9 subunits . - pubmed
The remaining sperm ( 6 % ) displayed a sharp spike of Ca2 + ( approximately 1 min ; 86 % + /- 4.3 SEM change in fluorescence intensity ) , followed by abrupt loss of fluorescence , a pattern suggestive of AR . - pubmed
Indeed , there is growing evidence that the gonadotropin , luteinizing hormone , which regulates serum estrogen and testosterone concentrations , could be an important causative factor in the development of Alzheimer ' s disease . - pubmed
Inhibition of indoleamine 2,3 dioxygenase activity by H2O2 . - pubmed
Indoleamine 2,3 - dioxygenase is the first and rate limiting enzyme of the kynurenine pathway of tryptophan metabolism , has potent effects on cell proliferation and mediates antimicrobial , antitumorogenic , and immunosuppressive effects . - pubmed
The work presented here represents the first systematic study exploring the mechanisms by which low levels of hydrogen peroxide ( 10 - 100 microM ) inhibit indoleamine 2,3 - dioxygenase in vitro . - pubmed
Following brief peroxide exposure both enzyme inhibition and structural changes were observed . - pubmed
Enzyme activity could in part be preserved in the presence of sulfhydryl containing compounds , particularly DTT and methionine . - pubmed
Some enzyme activity could be recovered in the presence of thioredoxin , indicating that the inhibitory effect of H ( 2 ) O ( 2 ) is at least partially reversible in vitro . - pubmed
We present evidence that cysteine oxidation represents one mechanism of indoleamine 2,3 - dioxygenase inhibition . - pubmed
Cessation of short - term alcohol administration thus had no promoting effects on development of GST - P foci , suggesting that the duration of alcohol treatment may be important . - pubmed
Our hypothesis that plant annexins mediate exocytotic events is supported by the finding that p23 , p33 , and p35 bind to these secretory vesicles in a Ca ( 2 +)-dependent manner . - pubmed
Homochiral 1D zinc - quitenine coordination polymer with a high dielectric constant . - pubmed
We further demonstrated that His384 , a universally conserved residue among protein tyrosine kinases , is not essential for Src catalysis or its inactivation by C - terminal tail Tyr phosphorylation . - pubmed
Interestingly , His384 mutants undergo autophosphorylation on Tyr416 like wild - type Src but are not activated by autophosphorylation . - pubmed
The potential role of His384 in Src activation by autophosphorylation is discussed in the context of Src structure . - pubmed
Increased cell survival and decreased chlorophyll degradation in transformants under acclimated conditions further confirmed the relevance of codA in imparting stress tolerance . - pubmed
Aer residues 210 to 290 were individually replaced with cysteine and then cross - linked in vivo . - pubmed
Several findings supported the hypothesis that Aer has a four - helix HAMP structure : ( i ) cross - linking independently identified the same residues at the dimer interface that were predicted by the model , ( ii ) the rate of cross - linking for residue pairs was inversely proportional to the beta - carbon distances measured on the model , and ( iii ) clockwise lesions that were not contiguous in the linear Aer sequence were clustered in one region in the folded HAMP model , defining a potential site of PAS - HAMP interaction during signaling . - pubmed
posadasii identification was confirmed by nested PCR of the antigen 2 / proline - rich antigen ( Ag2 / PRA ) encoding gene . - pubmed
An argatroban infusion was started at 2 microg / kg / minute during her 33rd week of pregnancy , with the dosage titrated based on the activated partial thromboplastin time ; infusion rates ranged from 2 - 8 microg / kg / minute . - pubmed
Periodic acid - Schif staining and immunohistochemical staining of CD34 were conducted to explore if VM exists in those Rb specimens . - pubmed
OBJECTIVE : The antiprogestin mifepristone is widely used for medical termination of pregnancy ( TOP ) . - pubmed
Medical TOP was induced with 200 mg of mifepristone followed by 0.8 mg of misoprostol 2 days later . - pubmed
Three - dimensional ultrasound examinations and hormone assays ( progesterone , human chorionic gonadotropin , and 17 - hydroxyprogesterone ) were performed in both groups on the day of , and 2 days after , administration of mifepristone . - pubmed
The esterification of cholesterol in plasma by LCAT was 67 % and in tissues by ACAT was 64 % . - pubmed
The relative esterification ( cholesterol being 1.0 ) of these sterols by the plasma LCAT was 1.00 , 0.95 , 0.89 , 0.40 , 0.85 , 0.82 and 0.80 , 0.69 and 0.82 , respectively . - pubmed
The predominant fatty acid of the sterol esters was linoleic acid for LCAT and oleic acid for ACAT . - pubmed
The saturation of the A ring of cholesterol increased ester formation by ACAT by 29 % and decreased the esterification by LCAT by 5.9 % . - pubmed
Esterification by ACAT and LCAT was reduced , respectively , by 25 and 11 % by the presence of an additional methyl group on the side chain of cholesterol at the C-24 position . - pubmed
This data supports our hypothesis that the structure of the sterol substrate has a significant effect on its esterification by ACAT or LCAT . - pubmed
Alternatively , we show through two - color spectro - electrochemistry that Raman scattering is also finely sensitive to oxidation state changes of flavin , a biomimetic system that mimics the active center of many flavoprotein enzymes . - pubmed
CONCLUSIONS : Our study confirmed a close association between preoperative glucose control indicated by HbA ( 1c ) levels & lt;7 % and a decreased risk of postoperative infectious complications . - pubmed
In critical patients sources of oxidative stress include the mitochondrial respiratory electron transport chain , xanthine oxidase activation , the respiratory burst associated with neutrophil activation , and arachidonic acid metabolism . - pubmed
Several endogenous antioxidants have been identified including enzymes , like superoxide dismutases and glutathione peroxidase , vitamins and other molecules such as uric acid and bilirubin . - pubmed
We present a case of metastatic CDC that responded favorably to a multiple tyrosine kinase inhibitor , sunitinib , achieving a partial response in both lung and skeletal metastases . - pubmed
Considering these findings , it would be worthwhile prospectively investigating the role of multiple tyrosine kinase inhibitors , particularly sunitinib , in the management of metastatic CDC . - pubmed
Muscarinic acetylcholine receptors in the nucleus accumbens core and shell contribute to cocaine priming - induced reinstatement of drug seeking . - pubmed
Muscarinic acetylcholine receptors in the nucleus accumbens play an important role in mediating the reinforcing effects of cocaine . - pubmed
However , there is a paucity of data regarding the role of accumbal muscarinic acetylcholine receptors in the reinstatement of cocaine - seeking behavior . - pubmed
The goal of these experiments was to assess the role of muscarinic acetylcholine receptors in the nucleus accumbens core and shell in cocaine and sucrose priming - induced reinstatement . - pubmed
Results indicated that systemic administration of the muscarinic acetylcholine receptor antagonist scopolamine ( 5.0 mg / kg , i.p . ) - pubmed
In contrast , infusion of scopolamine ( 36.0 mug ) directly into the accumbens core , but not shell , attenuated sucrose reinstatement , which suggests that muscarinic acetylcholine receptors in these two subregions of the nucleus accumbens have differential roles in sucrose seeking . - pubmed
Taken together , these results indicate that cocaine priming - induced reinstatement is mediated , in part , by increased signaling through muscarinic acetylcholine receptors in the shell subregion of the nucleus accumbens . - pubmed
The proposed model is successfully applied to estimate the site energy distribution of three pitch - based activated carbons ( PA , PFeA and PBA ) developed in our laboratory and also for other common adsorbent materials for different gas molecules . - pubmed
We planned this case - control study to evaluate the relationship between hypogonadism , as defined by the sexual hormone profile ( FSH , LH , testosterone ( T ) and prolactin ) and ED , as assessed by means of an internationally validated self - administered questionnaire ( IIEF ) . - pubmed
Mean alcohol consumption significantly differed in the three ALDH2 genotypes : 297 g per week in NN , 158 g in NM and 18 g in MM . - pubmed
These results suggest an interactive effect of ALDH2 and P450 2E1 genes on alcohol consumption and a higher sensitivity to alcohol - induced health effects in c2/2 genotypes , although larger scale studies are required to confirm these findings . - pubmed
Frataxin homologues are important iron chaperones in eukarya and prokarya . - pubmed
In a four - constituents reconstitution system ( including SufU , SufS , Fra and CitB ) we observed a Fra - dependent formation of a [ 4 Fe-4 S ] cluster on SufU that could be efficiently transferred onto the target apo - aconitase ( CitB ) . - pubmed
Purine / Pyrimidine nucleotides ratio ( OD 260 : OD 280 ) was also measured RESULTS : P5'N-1 deficient patients showed reduction in P5'N-1 activity ( Mean + - SD ; 4.06 + - 0.66 using an ELISA reader & amp ; 6.25 + - 1.37 using a spectrophotometer ) as compared to the normal control group ( ELISA reader : 13.24 + - 3.42 & amp ; Spectrophotometer : 18.25 + - 3.20 ) . - pubmed
Heterozygotes showed intermediate activity ( ELISA reader : 6.06 + - 0.48 & amp ; Spectrophotometer : 8.06 + - 1.28 ) , however they would have been missed on screening using the Purine / Pyrimidine nucleotides ratio CONCLUSION : Determination of P5'N-1 activity by using an ELISA reader is a new , simple , less time consuming and reliable method . - pubmed
MTS assay was used to measure the ability of DC transduced with AAV - AFP ( AAV - AFP + DC ) to stimulate the proliferation of T cell . - pubmed
For each SD increase in log FABP4 , hazard ratios ( HRs ) for diabetes were 1.35 ( 95 % CI 1.10 - 1.65 ) for women and 1.45 ( 1.13 - 1.85 ) for men controlling for age , race , education , physical activity , cystatin C , alcohol intake , smoking , self - reported health status , and estrogen use for women ( P for sex - FABP4 interaction 0.10 ) . - pubmed
circinelloides is beta - carotene , although it has some hydroxylase activity and produces low amounts of zeaxanthin . - pubmed
Two enzymatic activities are required for the production of astaxanthin from beta - carotene : a hydroxylase and a ketolase . - pubmed
Using site - directed mutagenesis and high - resolution copper - phenanthroline footprinting , we have identified the functional toxboxes required for the ToxT activation of PctxAB . - pubmed
AIM : To assess the effect of simulated mouth conditions reproduced with thermocycling on the tensile bond strength of two silicone based resilient denture liners with acrylic resin bases . - pubmed
Serum FSH , LH , and estradiol ( E2 ) were measured . - pubmed
In this study , we present a theory on how the CO2 diffusion gradient over the gasket is affected by leaf - mediated pores ( LMP ) and how LMP reduce diffusive exchange across the gaskets . - pubmed
However , a possible correlation between RBC MTX and MTXGlu2 - 5 concentrations and clinical outcomes of MTX treatment in RA is debated . - pubmed
AIM : The purpose of this analysis was to describe the pharmacokinetics of MTX and MTXGlu ( 2 - 5 ) in RBCs . - pubmed
Dexmedetomidine , a selective alpha2 - adrenergic receptor agonist , is often used for sedation and analgesia especially in intensive care units . - pubmed
Following this , local immune response was investigated by measuring cytokine levels in the presence of dexmedetomidine in spleen cell culture . - pubmed
According to our in vitro and in vivo results , dexmedetomidine shows anti - inflammatory effect by increasing IL-4 and IL-10 levels responsible from anti - inflammatory response via Th2 pathway . - pubmed
Moreover , we showed for the first time in the study that dexmedetomidine administration reduces IL-23 , which is responsible from initiation of inflammatory response via Th17 pathway . - pubmed
, a facultative C3 halophyte species , can efficiently control the activity of slow ( SV ) and fast ( FV ) tonoplast channels to match specific growth conditions by ensuring that most of accumulated Na + is safely locked in the vacuole ( Bonales - Alatorre et al . - pubmed
The interaction between an adsorbed 1 - butyl-1 - methylpyrrolidinium bis ( trifluoromethylsulfonyl ) imide , [ BMP ][ TFSA ] , ionic liquid ( IL ) layer and a Ag ( 111 ) substrate , under ultrahigh - vacuum conditions , was investigated in a combined experimental and theoretical approach , by high - resolution scanning tunneling microscopy ( STM ) , X - ray photoelectron spectroscopy ( XPS ) , and dispersion - corrected density functional theory calculations ( DFT - D ) . - pubmed
DHEAS , testosterone , FTI , SHBG , estriol , progesterone , beta - hCG , TSH , free T3 and free T4 concentrations did not differ between the groups . - pubmed
Pepsin in the gastric fluid would compete for the adsorption sites with the adsorbates , and bile salts in the intestinal fluid had a solubilization effect on the chemicals . - pubmed
However , as we reported herein , citric acid covalently modified a recombinant monoclonal antibody ( IgG1 ) in a phosphate / citrate - buffered formulation at pH 5.2 and led to the formation of so - called " acidic species " that showed mass increases of 174 and 156 Da , respectively . - pubmed
ST7612AA1 , a thioacetate - omega ( gamma - lactam carboxamide ) derivative selected from a novel generation of oral HDAC inhibitors . - pubmed
A systematic study of medicinal chemistry aimed at identifying a new generation of HDAC inhibitors , through the introduction of a thiol zinc - binding group ( ZBG ) and of an amide - lactam in the omega - position of the polyethylene chain of the vorinostat scaffold , allowed the selection of a new class of potent pan - HDAC inhibitors ( pan - HDACis ) . - pubmed
Molecular docking into HDAC crystal structures suggested a binding mode for these thiol derivatives consistent with the stereoselectivity observed upon insertion of amide - lactam substituents in the omega - position . - pubmed
ST7612AA1 ( 117 ) , selected as a drug candidate for further development , showed an in vitro activity in the nanomolar range associated with a remarkable in vivo antitumor activity , highly competitive with the most potent HDAC inhibitors , currently under clinical trials . - pubmed
Greater PAV regression in women versus men occurred with rosuvastatin ( p = 0.004 ) , those with diabetes ( p = 0.01 ) , stable coronary disease ( p = 0.01 ) , higher baseline LDL - C ( p = 0.02 ) , and higher CRP ( p = 0.04 ) levels . - pubmed
Annexin A5 promotes invasion and chemoresistance to temozolomide in glioblastoma multiforme cells . - pubmed
In the present study , we explored the effects of annexin A5 on GBM cell invasion and chemoresistance to temozolomide . - pubmed
Overexpression of annexin A5 in both cell lines significantly increased cell invasion , matrix metalloproteinase-2 ( MMP-2 ) expression / activity , Akt phosphorylation at serine 473 , and the half maximal inhibitory concentration ( IC50 ) values of temozolomide and markedly decreased temozolomide - induced apoptosis , all of which were abolished by selective PI3 K inhibitor BKM120 . - pubmed
On the other hand , knockdown of annexin A5 markedly decreased cell invasion , MMP-2 expression / activity , Akt phosphorylation at serine 473 , and the IC50 values of temozolomide and significantly increased temozolomide - induced apoptosis . - pubmed
In conclusion , our study provides the first evidence that annexin A5 promotes GBM cell invasion , MMP-2 expression / activity , and chemoresistance to temozolomide through a PI3K - dependent mechanism . - pubmed
PURPOSE : To report a subgroup analysis comparing safety and effectiveness outcomes in Japanese and non - Japanese patients as part of a prospective , multinational , randomized controlled trial ( ClinicalTrials.gov identifier NCT00120406 ) evaluating a paclitaxel - coated drug - eluting stent ( DES ) compared to percutaneous transluminal angioplasty ( PTA ) for treating peripheral artery disease . - pubmed
AIM : The treatment strategy for steroid - resistant nephrotic syndrome remains uncertain at present , especially in those with calcineurin inhibitor resistance or intolerance . - pubmed
A commercial CSF vaccine ( C - strain ) and phosphate - buffered saline ( PBS ) were used as positive or negative controls , respectively . - pubmed
On hydrophilic Si , adhesion increased with increasing RH for pollens with or without pollenkitt , up to 200nN at 70 % RH . - pubmed
Additionally , 3.8 % of the rifampin - resistant isolates showing no mutation in the rpoB gene were significantly associated with mixed infections ( chi(2 ) , 56.78 ; P & lt ; 0.01 ) . - pubmed
This study revealed for the first time that Manu2 was the predominant group in the cases of mixed infections , and this might be the main reason for heteroresistance and a possible mechanism for isolates without any mutation in the rpoB gene to become rifampin resistant . - pubmed
The most interesting formulation , containing GCS and SBE - beta - CD , was denoted as DA GCS / DA - CD NPs . - pubmed
DA GCS / DA - CD NPs showed cytotoxic effect toward Olfactory Ensheathing Cells only at higher dosage . - pubmed
Design and Synthesis of Imidazopyrazolopyridines as Novel Selective COX-2 Inhibitors . - pubmed
In the present study , we introduced imidazopyrazolopyridines as new potent and selective COX-2 inhibitors that lack the standard pharmacophoric binding features to hERG . - pubmed
This work examined the potential of the anodic iontophoresis of doxorubicin - loaded cationic solid lipid nanoparticles ( DOX - SLN ) to increase the distribution and tumor penetration of DOX . - pubmed
A double - labeled cationic DOX - SLN composed of the lipids stearic acid and monoolein and a new BODIPY dye was prepared and characterized . - pubmed
Although the iontophoresis of a DOX solution increased the penetration of DOX in the viable epidermis by approximately 4 - fold , the iontophoresis of cationic DOX - SLN increased the DOX penetration by approximately 50 - fold . - pubmed
In vivo , the DOX - SLN iontophoretic treatment was effective in inhibiting tumor cell survival and tumor growth and was accompanied by an increase in keratinization and consequent cell death . - pubmed
These results indicate a strong and synergic effect of iontophoresis with DOX - SLN and provide a potential strategy for the treatment of skin cancer . - pubmed
In recent years , gefitinib , an epidermal growth factor receptor tyrosine kinase inhibitor , has shown its good antitumor activities in treating NSCLC in a number of studies . - pubmed
LncRNA GClnc1 Promotes Gastric Carcinogenesis and May Act as a Modular Scaffold of WDR5 and KAT2A Complexes to Specify the Histone Modification Pattern . - pubmed
Mechanistically , GClnc1 bound WDR5 ( a key component of histone methyltransferase complex ) and KAT2A histone acetyltransferase , acted as a modular scaffold of WDR5 and KAT2A complexes , coordinated their localization , specified the histone modification pattern on the target genes , including SOD2 , and consequently altered gastric cancer cell biology . - pubmed
High throughput chemical library screening identifies a novel p110 - delta inhibitor that potentiates the anti - myeloma effect of bortezomib . - pubmed
Genetic or pharmacologic inhibition of p110 - delta substantially reduced myeloma viability and enhanced cellular sensitivity to bortezomib . - pubmed
A WD40 protein , AtGHS40 , negatively modulates abscisic acid degrading and signaling genes during seedling growth under high glucose conditions . - pubmed
The ghs40 - 1 mutant displayed severely impaired cotyledon greening and expansion and showed enhanced reduction in hypocotyl elongation of dark - grown seedlings when grown in Glc concentrations higher than 3 % . - pubmed
The Glc - hypersensitivity of ghs40 - 1 was correlated with the hyposensitive phenotype of 35S::AtGHS40 seedlings . - pubmed
Quantitative real time - PCR analysis showed that AtGHS40 was involved in the control of Glc - responsive genes . - pubmed
AtGHS40 acts downstream of HXK1 and is activated by ABI4 while ABI4 expression is negatively modulated by AtGHS40 in the Glc signaling network . - pubmed
Given that AtGHS40 inhibited ABA degrading and signaling gene expression levels under high Glc conditions , a new circuit of fine - tuning modulation by which ABA and ABA signaling gene expression are modulated in balance , occurred in plants . - pubmed
Thus , AtGHS40 may play a role in ABA - mediated Glc signaling during early seedling development . - pubmed
The cells were also transfected with small interfering RNA directed against PLTP , and U0126 , an inhibitor of the ERK1 / 2 pathway , was administered before CSE exposure . - pubmed
Topoisomerase 1 ( TOP1 ) poisons like camptothecin ( CPT ) are currently used in cancer chemotherapy but these compounds can have damaging , off - target effects on neurons leading to cognitive , sensory and motor deficits . - pubmed
Neuronal cells expressed higher levels of Top1 mRNA than fibroblasts but transcript levels are reduced in all cell types after treatment with CPT . - pubmed
Pathway analysis of the differentially expressed transcripts revealed activation of ERK and JNK signaling cascades in CPT - treated MNs . - pubmed
Immediate - early genes like Fos , Egr-1 and Gadd45b were upregulated in CPT - treated MNs . - pubmed
Fos mRNA levels were elevated in all cell types treated with CPT ; Egr-1 , Gadd45b and Dyrk3 transcript levels , however , increased in CPT - treated MNs and CNs but decreased in CPT - treated fibroblasts . - pubmed
Supplementation of drinking water with 40 mg / L ( 6 mg / kg BW ) boron also markedly increased splenic IL-2 expression and the CD4+/CD8 + cell ratio and reduced splenic CD8 + cell number . - pubmed
Supplementation with 80 mg / L ( 12 mg / kg BW ) boron significantly increased CD3 + and PCNA+ cell numbers ( P & lt ; 0.05 ) and decreased the IL-10 expression in the spleen . - pubmed
Addition of 320 ( 48 ) and 640 ( 96 ) mg / L ( mg / kg BW ) boron markedly reduced the serum IgG concentrations ; splenic IL-2 and IL-10 expression ; the number of CD3 + , CD4 + and PCNA+ cells ; and increased the number of splenic CD8 + and caspase-3 + cells and promoted caspase-3 expression in CD3 + cells . - pubmed
SUMMARY : Background FXaI16L ( PF-05230907 ) is a zymogen - like variant of activated factor X ( FXa ) . - pubmed
PREDICTORS : Creatinine , cystatin C , beta - trace protein ( BTP ) , and beta2 - microglobulin ( B2 M ) were measured in serum samples collected at the 12- and 24 - month follow - up visits , along with measured GFR ( mGFR ) at these time points . - pubmed
CONCLUSIONS : Declines in mGFR , eGFRcr , eGFRBTP , and the average of 4 filtration markers ( creatinine , cystatin C , BTP , and B2 M ) were consistently associated with progression to ESRD . - pubmed
In ~50 % of prostate cancers , chromosomal rearrangements cause the fusion of the promoter and 5'-UTR of the androgen - regulated TMPRSS2 ( transmembrane protease , serine 2 ) gene to the open reading frame of ERG , encoding an ETS family transcription factor . - pubmed
Here , we report a mechanism whereby ERK - mediated phosphorylation of ERG at one serine residue causes a conformational change that allows ERK phosphorylation at a second serine residue , Ser-96 . - pubmed
We found that the Ser-96 phosphorylation resulted in dissociation of EZH2 and SUZ12 , components of polycomb repressive complex 2 ( PRC2 ) , transcriptional activation of ERG target genes , and increased cell migration . - pubmed
Conversely , loss of ERG phosphorylation at Ser-96 resulted in recruitment of EZH2 across the ERG - cistrome and a genome - wide loss of ERG - mediated transcriptional activation and cell migration . - pubmed
Sialic acid - binding lectins ( SABLs ) are ubiquitous ancient molecules with binding properties to N - acetyl or N - glycolyl carbohydrates , and play crucial roles in both adaptive and innate immune responses . - pubmed
Syndecan-1 ( Sdc1 ) is a major cell surface heparan sulfate ( HS ) proteoglycan of epithelial cells , a cell type targeted by many bacterial pathogens early in their pathogenesis . - pubmed
Synergistic antiproliferative effects of an mTOR inhibitor ( rad001 ) plus gemcitabine on cholangiocarcinoma by decreasing choline kinase activity . - pubmed
This study aims to investigate a novel therapeutic regimen [ gemcitabine plus everolimus ( rad001 ) , an mTOR inhibitor ] for cholangiocarcinoma . - pubmed
In the in vitro study , gemcitabine plus rad001 exerted a synergistic therapeutic effect on the cholangiocarcinoma cells , irrespective of the KRAS mutation status . - pubmed
In the xenograft study , gemcitabine plus rad001 showed the best therapeutic effect on tumor volume change , and was associated with increased caspase-3 expression , decreased eIF4E expression , as well as overexpression of both death receptor- and mitochondrial apoptotic pathway - related genes . - pubmed
Also , increased intratumoral free choline , decreased glycerophosphocholine and nearly undetectable phosphocholine levels were demonstrated by proton nuclear magnetic resonance , supported by results of decreased choline kinase expression in western blotting . - pubmed
We concluded that gemcitabine plus rad001 has a synergistic antiproliferative effect on cholangiocarcinoma , irrespective of the KRAS mutation status . - pubmed
The antitumor effect is associated with activation of both death receptor and mitochondrial pathways , as well as the downregulation of choline kinase activity , resulting in a characteristic change in choline metabolism . - pubmed
Although TCR specificity seems to be important for CBZ - induced SJS / TEN in South East Asian patients , a distinct repertoire may not play a role in reactions to other drugs . - pubmed
CPSase is a metabolic enzyme that is involved in pyrimidine and arginine synthesis . - pubmed
Disruption of carB resulted in survival rates that were reduced by up to 2,500 - fold following antibiotic treatment , and this phenotype was abolished by the addition of uracil , highlighting the importance of de novo pyrimidine biosynthesis for persister formation . - pubmed
Disruption of carB resulted in intracellular ATP accumulation , and lowering ATP levels using arsenate restored the antibiotic tolerance profile of the mutant to levels similar to those seen with the wild type . - pubmed
aeruginosa Disruption of carB resulted in a metabolic perturbation that increased cellular ATP and reduced persister formation . - pubmed
While PBA treatment reduced ER stress and increased collagen IV incorporation into BMs , the persistence of defects in BM structure and reduced ability of the BM to withstand mechanical stress indicate that PBA may be counter - indicative for pathologies caused by matrix defects . - pubmed
Furthermore , to evaluate the molecular mechanism involved in cancer cell death , we studied the genes that are involved in fatty acid and cholesterol biosynthesis pathway including 3 - hydroxy-3 - methylglutaryl - coenzyme A reductase ( HMGCR ) , Acetyl CoA synthase ( ACC ) , sterol regulatory element - binding protein ( SPREPB1 ) and fatty acid synthase ( FASN ) respectively . - pubmed
Conformational sampling and polarization of Asp26 in pKa calculations of thioredoxin . - pubmed
Thioredoxin is a protein that has been used as model system by various computational methods to predict the pKa of aspartate residue Asp26 which is 3.5 units higher than a solvent exposed one ( eg , Asp20 ) . - pubmed
Here , we use extensive atomistic molecular dynamics simulations of two different protonation states of Asp26 in combination with conformational analysis based on RMSD clustering and principle component analysis to identify representative conformations of the protein in solution . - pubmed
For each conformation , the Gibbs free energy of proton transfer between Asp26 and Asp20 , which is fully solvated in a loop region of the protein , is calculated with the Amber99sb force field in alchemical transformations . - pubmed
The inclusion of the polarization of both aspartate residues in the free energy cycle by HI atomic charges corrects the results from the non - polarizable force field and reproduces the experimental DeltapKa value of Asp26 . - pubmed
Additionally , we investigated the effects of blocking NMDA or non - NMDA parietal ionotropic glutamate receptors on TUNL and found that , in contrast to the prefrontal cortex , NMDA receptors in the PPC are not necessary for TUNL performance , whereas blockade of AMPA / Kainate receptors significantly impaired accuracy . - pubmed
Moreover , a significant increment ( p & lt ; 0.05 ) in the levels of AST , ALP , creatinine , and urea was evident in both experimental groups but more significant differences were noticed in the tartrazine group . - pubmed
Tapentadol is a novel analgesic molecule that synergistically combines two mechanisms of action , micro - opioid receptor agonism and norepinephrine reuptake inhibition , and for which multiple literature data are available that confirm its efficacy and safety in controlling pain . - pubmed
The compounds namely 2 ( R ) , 4 ( R )- dihydroxypyrrolidine , 4 - hydroxy-2 -( 4 - methoxyphenyl )- 1,1 - dioxo-3,4 - dihydrothieno [ 3,2 - e ] thiazine-6 - sulfonamide , 2,3 - Diketo - L - gulonic acid , ( 3 - hydroxy-2 - octadeca-9,12 - dienoyloxypropyl ) octadecanoate , 2 -[[ 4 -[[ 4 -[( 4 - formamido-1 - methylimidazole-2 - carbonyl ) amino ]- 1 - methylimidazole-2 - carbonyl ] amino ]- 1 - methylimidazole-2 - carbonyl ] amino ] ethyl - dimethylazanium , Picolinic acid and ( 2 - Hydroxy-5 - nitrophenyl ) dihydrogen phosphate successfully bind inside the pocket of survivin . - pubmed
The presence of two reduced function variants in CYP2C9 influences the acute response to glipizide . - pubmed
Glucose and insulin levels were measured at baseline , 30 , 60 , 90 , 120 , 180 and 240 min for calculation of phenotypic endpoints of glipizide response . - pubmed
CONCLUSIONS : Carriers of two reduced function alleles in CYP2C9 may experience an increased insulin response to glipizide and be predisposed to a higher risk of hypoglycaemia , although no effect of genotype was seen in glucose - based measurements . - pubmed
Further studies are needed to clarify the utility of CYP2C9 genotyping to guide sulfonylurea treatment . - pubmed
: hypromellose phthalate ( HP ) , hypromellose ( AN ) , and hypromellose ( CN ) along with acetic acid ( AA ) ( 0.1 M , 20 % w / v ) as a plasticizer . - pubmed
The bioaccessibility of the total phenolic content and the total flavonoids - including decursin ( D ) and decursinol angelate ( DA)-were significantly higher in HP - AA - EAGN compared to the control . - pubmed
In whole - cell voltage - clamp recording , ASIC currents were evoked by brief local application of pH 6.0 external solution in the presence of TRPV1 channel blocker AMG9810 . - pubmed
Construction of a recyclable oxidase - mimicking Fe3O4@MnOx - based colorimetric sensor array for quantifying and identifying chlorophenols . - pubmed
Herein , we report a recyclable colorimetric sensor array according to the oxidase - mimicking catalytic characteristics of Fe3O4@MnOx for the high - performance quantification and differentiation of typical CPs . - pubmed
The core - shell Fe3O4@MnOx prepared by growing oxidase - like MnOx nanoflakes on the surface of magnetic Fe3O4 particles via a hydrothermal process can exhibit excellent catalytic activity to trigger the color reaction of CPs and 4 - aminoantipyrine with the participation of O2 . - pubmed
Objectives : The aim of our study was to develop a simple , precise , sensitive and specific method for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products using GC - MS . - pubmed
Materials and Methods : The methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of arbutin and hydroquinone using GC - MS method . - pubmed
Here , a hybrid PSC with a graded band gap is explored using MAPbBr3 ( MA = CH3NH3 ) and MAPbBr0.9I2.1 quantum dots ( QDs ) as component cells . - pubmed
Further analyses showed that CvBV-7 - 1 and three homologs from other Cotesia bracoviruses possessed a C - terminal leucine / isoleucine - rich region and had a similar function in inhibiting melanization . - pubmed
Our findings suggest that CLP genes from Cotesia bracoviruses encoded proteins that contain a C - terminal leucine / isoleucine - rich region and function as melanization inhibitors during the early stage of parasitization , which is important for successful parasitization . - pubmed
lncRNA - PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1 / NF - kappaB Signaling Pathway . - pubmed
In this study , the authors explored PRLB effect in the paclitaxel ( Tax ) resistance of ovarian cancer cells and revealed the role of RSF1 ( remodeling and spacing factor 1)/nuclear factor kappaB ( NF - kappaB ) signaling in this process . - pubmed
Nowadays , LDs are viewed as dynamic organelles , which store and release fatty acids depending on energy demand ( LD dynamics ) . - pubmed
We aimed to quantify PLIN2 and PLIN5 content at individual LDs as a reflection of the ability to switch between fatty acid release and storage depending on energy demand . - pubmed
Herein , at first , its main active components were identified by UPLC - ESI - qTOF - MS , and a representative MF flavone glycosides profile consisting of ten compounds was illustrated , which is the most detailed one up to now . - pubmed
Cholesterol glucosylation serves several functions that include promoting immune evasion , enhancing antibiotic resistance , maintaining the native helical morphology , and supporting functions of prominent virulence factors such as CagA and VacA. - pubmed
Body weight , disease activity index ( DAI ) , colon length , biochemical parameters ( MDA and SOD ) , and histopathological score in colon tissue were measured . - pubmed
Metabolomics results revealed forty - nine differential metabolites as biomarkers of DSS - induced colitis , demonstrating that the path - mechanism of colitis involved the perturbation of eight metabolic pathways , including alpha - linolenic acid and linoleic acid metabolism , sphingolipid metabolism , retinol metabolism , bile acid metabolism , et al . - pubmed
Thirty - six biomarkers were especially reversed to normal - like levels by PQ - A via regulation of alpha - linolenic acid and linoleic acid metabolism , sphingolipid metabolism , and retinol metabolism , which effectively hinted the potential pharmacological mechanism of PQ - A related to NF - kappaB / p65 inflammatory signaling . - pubmed
Molecular docking results predicted high affinity interaction between PQ - A and NF - kappaB / p65 , involving hydrogen - bond interactions at five amino acid residues , suggesting NF - kappaB / p65 as a target . - pubmed
Butylated hydroxytoluene ( BHT ) and butylated hydroxyanisole ( BHA ) at concentrations of 100 ppm ( 0.45 and 0.55 mM , respectively ) produced a marked leakage of lactic dehydrogenase ( LDH ) from cultured myocardial and endotheloid cells into the culture medium . - pubmed
Two and four days after adrenalectomy ( ADX ) on either day 5 or 10 after birth , a significant decrease in the concentration of FSH was found ; 4 days after ADX on either day 15 or 20 , FSH concentrations had increased significantly compared with sham - operated and/or intact controls . - pubmed
It is argued that the effects of ADX on both the release of gonadotrophins and the onset of puberty are primarily , and presumably exclusively , due to the effects on general bodily development ( expressed in body weight ) . - pubmed
The enthalpy change for the oxidative deamination of glutamate by NADP+ catalyzed by bovine liver glutamate dehydrogenase has been determined calorimetrically . - pubmed
[ Molecular weight distribution of hydrolysis products of polyuridylic acid by cobra venom ribonuclease . - pubmed
Onset of spontaneous labor and changes in E2 and progesterone levels -- possible interrelation . - pubmed
Angiotensin II or endothelin-1 was continuously infused into the renal artery throughout the experiment and a bolus of torasemide or furosemide was injected into the bracheal vein . - pubmed
infusion of endothelin-1 , 1.5 ng kg-1 min-1 , produced effects similar to those of angiotensin II on renal haemodynamics ; however , the onset of action was extremely slow compared with the effects produced by angiotensin II . - pubmed
alpha - Fetoprotein ( AFP ) production by 10(4 ) cells in 48 h increased in the A-10 Injection - treated and A-10 Injection+CDDP - treated groups as the concentration of these agents increased . - pubmed
Analysis of the sulfated molecules by chondroitinase ABC digestion followed by composite agarose / acrylamide gel electrophoresis confirmed the proteoglycan nature of the molecules . - pubmed
Immunoprecipitation of the methionine - labeled extracts with a polyclonal antibody against the core protein indicated that the reduction in glycosaminoglycan synthesis was due to an inhibition of the core protein synthesis after IL-1 treatment . - pubmed
In this study we compared 2 putative biochemical markers for rejection of pancreas allografts , serum immunoreactive anodal trypsinogen and urinary amylase , with serum creatinine in 15 simultaneously transplanted type I diabetics . - pubmed
These descending limbic structures , together with the amygdala , further send efferents to the four ascending NA , 5 - HT , DA and ACh systems . - pubmed
Therefore , the coupling of these descending limbic structures to the ascending NA , 5 - HT , DA and ACh systems completes the neural circuits responsible for the consolidation and retrieval of new declarative memories . - pubmed
ISDN and its two main hepatic metabolites , isosorbide-2 - mononitrate ( IS-2 - MN ) and isosorbide-5 - mononitrate ( IS-5 - MN ) were compared for their ability to inhibit platelet aggregation and thromboxane B2 generation in vitro in response to threshold concentrations of ADP , adrenaline , collagen , thrombin and arachidonic acid . - pubmed
Generation of a platelet - active species , IS-2 - MN , and synergism with prostacyclin are novel properties of ISDN and may account for in vivo - in vitro differences in antiaggregatory properties and in vivo mechanism of action . - pubmed
Effects of estrogen on progesterone receptor synthesis during endometrial cell culture . - pubmed
The increase in the progesterone receptor depended on the concentration of estradiol , with maximal response at about 1 nM. - pubmed
The progesterone receptor response was specific for estrogen . - pubmed
Testosterone or cortisol induced small or no response on the production of progesterone receptors . - pubmed
The progesterone receptor synthesis was inhibited by cycloheximide ( 10 micrograms / ml ) or actinomycin D ( 10 micrograms / ml ) . - pubmed
lysylbradykinin ) were inhibited in the presence of thapsigargin , whereas kinin responses were potentiated if the peptide was added following a stimulus which increases cyclic AMP . - pubmed
When examined by polarimetry , the material in all three fractions revealed native collagen helical structure with melting points which ranged from 31 - 37 degrees C. When the fractions were denatured and rechromatographed on a column of agarose beads , the most acidic fraction eluted as 13 - kDa polypeptides with and without prior reduction and alkylation . - pubmed
Two 13 - kDa polypeptides , 13K2 and 13K3 , which did not contain any methionyl residues and were not cleaved with CNBr , were digested with trypsin , and the peptide digests were resolved by reversed - phase chromatography . - pubmed
To clarify the physiological role of placental corticotropin - releasing hormone ( CRH ) , we measured plasma CRH , ACTH , and cortisol throughout pregnancy . - pubmed
Maternal plasma CRH levels , which increased progressively during pregnancy , correlated well with both ACTH and cortisol in early labor , delivery , and postpartum samples , and also with cortisol levels in samples before labor . - pubmed
Role of insulin and counter - regulatory hormones in the control of hepatic glycerolipid synthesis and low - density - lipoprotein catabolism in diabetes . - pubmed
We found that the HA gene of the H4 subtype is 1738 nucleotides in length and is predicted to encode a polypeptide of 564 amino acids . - pubmed
The connecting peptide , which is removed from the precursor polypeptide by peptidases to yield the mature HA1 and HA2 polypeptides , contains only one basic amino acid . - pubmed
Prelabeled [ 3H ] AA platelets were stimulated with thrombin or collagen and the amount of free [ 3H ] AA and radiolabeled metabolites released were measured by high - performance liquid chromatography . - pubmed
The reduction in free [ 3H ] AA was accompanied by an increase ( P less than 0.01 ) in the percentage of 3H - radioactivity released as cyclooxygenase products in atopic platelets ( compared to nonatopic cells ) after stimulation with 10 and 25 micrograms / ml collagen . - pubmed
Structural modifications of metyrapone , an inhibitor of cytochrome P-450 enzyme systems , have been made to determine the requirement for the A - or B - pyridyl ring for inhibition of P-45011 beta and P-450 scc activities . - pubmed
Characterization of the mitochondrial preparation demonstrated : enhancement of both activities by the addition of 15.0 microM adrenodoxin , the addition of 1 % ethanol decreased both activities less than 10 % , and the apparent Km of deoxycorticosterone for P-45011 beta was 6.8 microM and the apparent Km of cholesterol for P-450 scc was 21.6 microM. - pubmed
Inhibition of P-45011 beta and P-450 scc activities with these compounds demonstrated : the B - pyridyl ring of metyrapone is required for inhibition of both activities whereas requirement for the A - ring is less stringent , and the four metyrapone analogs were more selective inhibitors of P-45011 beta activity . - pubmed
Both types of granules stained for basic protein and arginine . - pubmed
Comparison of amino - acid sequences of hypothalamic peptide , brain - specific histone and myelin basic protein . - pubmed
A comparative study of interferon ( IFN ) production ( type - alpha and gamma ) was carried out using Ficoll - hypaque purified fresh and cryopreserved mononuclear cells from eight normal healthy individuals . - pubmed
Cells carrying one of the phoA+ plasmids overproduce alkaline phosphatase 10 - fold upon phosphate limitation . - pubmed
aeruginosa , which increased in the presence of the iron - binding proteins lactoferrin and transferrin . - pubmed
This bacteriostasis was reduced when the iron - binding proteins were saturated with iron . - pubmed
We conclude that opiate receptor stimulation by morphine causes inhibition of the vagally mediated component of water - induced bronchoconstriction . - pubmed
The effect of S-100a and S-100b proteins and Zn2 + on the assembly of brain microtubule proteins in vitro . - pubmed
The homologous proteins S-100a and S-100b affect the microtubule system in a distinctly different way in the presence of low molar ratios of Zn2 + . - pubmed
Assembly of brain microtubule proteins can be almost completely inhibited and rapid disassembly can be induced by low molar amounts of S-100b in the presence of low molar ratios [ 2 - 4 ] of Zn2 + . - pubmed
S-100a or S-100b can not bind to the microtubule polymer - form , either in the absence or in the presence of Zn2 + . - pubmed
It is suggested that HSG in DES exposed women with fertility disturbances be performed early in the process of infertility investigation and cerclage should be considered when uterine anomalies are demonstrated . - pubmed
Cyclophosphamide ( CP ) is associated with significant pulmonary toxicity ; however , the mechanism of toxicity is unknown . - pubmed
Upon activation , CP produces 4 - hydroxycyclophosphamide , acrolein ( ACR ) and the alkylating metabolite , phosphoramide mustard . - pubmed
These studies suggest 1 ) CP is not metabolized within pulmonary artery endothelial cells , 2 ) ACR may be the principal CP metabolite involved in mediating direct injury to pulmonary artery endothelial cells and 3 ) sulfhydryl - rich agents may be effective in reducing CP - induced damage to critical endothelial cell barriers . - pubmed
A study of colchicine tubulin complex by donor quenching of fluorescence energy transfer . - pubmed
The tubulin - colchicine complex was used as a donor - acceptor system to show that two inaccessible tryptophans are at or near the colchicine binding site . - pubmed
PhoV consists of 550 amino acid residues , resulting in a molecular mass of 61.3 kDa . - pubmed
In contrast to several other alkaline phosphatases , PhoV was inhibited by Mn2 + . - pubmed
Angiotensin II , aldosterone and endothelins play a central role in the remodeling of the ECM in hypertension , and decrease collagenase activity and/or increase collagen synthesis in cultured cells . - pubmed
Cytokines may stimulate or inhibit cell growth , the latter through prostaglandin formation . - pubmed
Cardiac fibroblasts possess G - protein - coupled AT1 receptors for angiotensin II that couple to activation of multiple signalling pathways , including : phospholipase C - beta , with the subsequent release of Ca2 + from intracellular stores and activation of protein kinase C , mitogen - activated protein kinases , tyrosine kinases , phospholipase D , phosphatidic acid formation , and the STAT family of transcription factors . - pubmed
Autoradiographic analysis of nondenaturing polyacrylamide gel electrophoregrams of outer membrane preparations previously incubated with [ 35Fe]ferric vibrioferrin revealed a single radiolabeled band , in which the 78 - kDa protein was detected predominantly by sodium dodecyl sulfate - polyacrylamide gel electrophoresis . - pubmed
The treated cells lost most of their iron uptake activity mediated by vibrioferrin . - pubmed
These results suggest that the ferric vibrioferrin - binding protein of 78 kDa may function as the receptor for ferric vibrioferrin involved in the initial step of vibrioferrin - mediated iron uptake . - pubmed
The cells did not stain with acetylated low density lipoprotein conjugated to the fluorescent marker octadecyl indocarbocyanine , a marker for endothelial cells and monocytes . - pubmed
10 - Formyltetrahydrofolate dehydrogenase ( 10 - FTHFDH ) is a folate - binding protein that is important in folate metabolism . - pubmed
Upon isoproterenol stimulation , beta1- and beta2AR down - regulated with a half - life of about 3 h and the beta3AR with a half - life of 30 - 40 h. Long term stimulation with both saturating ( micromolar ) and nonsaturating ( nanomolar ) concentrations of beta - adrenergic agonists caused a complete desensitization of the beta - adrenergic response at the adenylyl cyclase level and loss of stimulated protein kinase A activity and CREB phosphorylation . - pubmed
These results suggest that cAMP - dependent processes will be desensitized upon permanent treatment with beta3AR agonists . - pubmed
Prior to uptake into the cell thiamin phosphates are first hydrolysed and thiamin is taken up as free vitamin which is then pyrophosphorylated by a pyrophosphokinase . - pubmed
The THI genes encoding the enzymes of these final steps of ThDP production and of thiamin utilisation have been identified . - pubmed
Their expression is controlled by the level of thiamin and a number of regulatory proteins involved in regulated expression of the THI genes are known . - pubmed
Eleven such proteins have been found : pyruvate decarboxylase ( Pdc , three isoforms ) , acetolactate synthase , a putative alpha - ketoisocaproate decarboxylase with a regulatory role in ThDP synthesis and two proteins of unknown function form the group of Pdc related enzymes . - pubmed
Surprisingly , it has been found recently that expression of one of the genes for Pdc is repressed by thiamin . - pubmed
Reactivity of the dimple surface with specific lectins suggests that fucose might be utilized as a marker of glycoproteins located at the dimple surface . - pubmed
The alpha - l - fucose - specific lectin Ulex europaeus agglutinin I was utilized both in lectin blotting and in affinity chromatography and cross - reacted with the 200- and 270/260 - kDa bands . - pubmed
The proteolytic cleavage was significantly reduced by the addition of an excess amount of l - arginine ( & gt;/=0.2 M ) to the culture medium , which resulted in a marked improvement in the yield of intact hPTH . - pubmed
The results demonstrated that l - arginine at high concentrations reduced the rate of hPTH proteolysis by inhibiting extracellular proteases . - pubmed
Using a combination of electrospray mass spectrometry , HPLC , direct sequencing , and family screening with polymerase chain reaction / restriction digestion approach , we have characterized this hemoglobin variant as resulting from a Thr --> ; Ala replacement at beta84(EF8 ) . - pubmed
The data obtained are in agreement with differently aimed stress - induced alterations in the activity of the membrane neutral sphingomyelinase : the key enzyme of the sphingomyelin cascade , in the membrane P2 fraction of the brain cortex . - pubmed
The IL-1 seems to participate in physiological mechanisms of realisation of stress reactions on the levels of its production and biological action on target cells as well as of the sphingomyelin pathway of its signal transduction in nerve tissue . - pubmed
Tissues have multiple proteolytic systems , including the ATP- and ubiquitin - dependent proteasome pathway . - pubmed
Insulin inhibited ATP- and ubiquitin - dependent lysozyme degradation more than 90 % by reticulocyte extract , in a dose - dependent manner ( IC50 approximately 50 nM ) . - pubmed
Glycerol gradient analysis revealed cosedimentation of IDE with the 20S proteasome and possibly with the 26S proteasome . - pubmed
Serum levels of alpha - tocopherol , lipid peroxidation products ( thiobarbituric acid ; TBA ) , creatine kinase ( CK ) , lactate dehydrogenase ( LDH ) , and LDH isozyme 1 - 5 were quantitatively analyzed . - pubmed
These results indicate that vitamin E supplementation can reduce the leakage of CK and LDH following 6 successive days of endurance running . - pubmed
The ability of various types of microcrystalline cellulose ( MCC ) to hold water when subjected to an applied force has been assessed by a centrifuge technique . - pubmed
Results indicated that lung cells could activate DADDS to dapsone and that , while the complex appeared to delay cell growth in the first 24 h period , no significant difference was seen between cells incubated in the presence and absence of the complex at 72 h. These results indicate that DADDS - CD has significant potential as a drug - delivery system for DADDS in the lung based upon the ability of the cells to activate DADDS . - pubmed
The protein composition of the stimulated cells was compared by sodium dodecyl sulfate - polyacrylamide gel electrophoresis and tyrosine phosphorylation was detected by Western blot . - pubmed
Calcium phosphate crystal - associated proteins : alpha-2 - HS - glycoprotein , prothrombin fragment 1 and osteopontin . - pubmed
The incubation of PPP with thrombin in the presence of 100 - 1000 nM aptamer resulted in the significant concentration - dependent prolongation of thrombin time ( up to fourfold , P<.0001 ) . - pubmed
UNLABELLED : The development of arterial hypertension is accompanied by impairment of the normal ratio of the " ACE - depending " and of the sympathetic nervous system , correlates with the action on the main pharmacological " targets " : I - I - imidazoline receptors ( moxonidine ) or the ACE activity ( enalapril ) . - pubmed
Spinal PGE ( 2 ) application facilitated dorsal horn neuronal firing elicited by ionophoretic delivery of NMDA , suggesting that an interaction of PGs and NMDA receptors may contribute to inflammation - induced central sensitization . - pubmed
RESULTS : Two different doses of CHM could significantly increase the area of the peritoneal lymphatic stomata , promote its distributive density and enhance the drainage of urinary ion such as sodium , potassium and chlorine . - pubmed
Meanwhile , the NO concentration of two different doses of CHM groups was 133.52+/-23.57 micromol / L and 137.2+/-26.79 micromol / L respectively . - pubmed
The effect of Chinese herbal medicine on the peritoneal lymphatic stomata and the drainage of urinary ion was altered by adding NO donor(sodium nitropurruside , SNP ) or NO synthase ( NOS ) inhibitor ( N ( G )- monomethyl - L - arginine , L - NMMA ) to the peritoneal cavity . - pubmed
Maple syrup urine disease ( MSUD ) is an inborn error of metabolism stemming from a deficiency in 2 - ketoacid dehydrogenase and resulting in the systemic accumulation of branched chain amino acids ( BCAAs ) . - pubmed
Remarkably , however , digit duplication by dHAND requires neither the transcriptional activation domain nor the basic region , but only the HLH motif . - pubmed
More than one caspase was involved and these caspases appeared to transmit parallel signals for apoptosis induction because only broad - spectrum caspase inhibitors , but not inhibitors for specific individual caspases inhibited apoptosis in choline- or methionine - deprived cells . - pubmed
reinhardtii CC-2341 ( ac - u - g-2.3 ) , a PSI - minus strain , fluorescence increased with chlorophyll without treatment with DCMU . - pubmed
We therefore expressed and tested CV - N homologs in which the glycosylation - susceptible Asn residue at position 30 was replaced with Ala , Gln , or Val , and/or the Pro at position 51 was replaced by Gly to eliminate potential conformational heterogeneity . - pubmed
Immunofluorescence studies on transiently transfected cells indicated membrane localization and indicated that both NH ( 2 )- and COOH - terminal tails of the SLC26A6 variants are located intracellularly , suggesting a topology with an even number of transmembrane domains . - pubmed
We demonstrated also that the COOH terminus of SLC26A6 binds to the first and second PDZ domains of the Na (+)/ H (+ ) exchanger ( NHE ) 3 kinase A regulatory protein ( E3KARP ) and NHE3 regulatory factor ( NHERF ) proteins in vitro . - pubmed
Truncation of the last three amino acids ( TRL ) of SLC26A6 abrogated the interaction but did not affect transport function . - pubmed
CONCLUSIONS : This study suggests that outpatient induction of labour with intravaginal CR - PGE2 may be a reasonable option for selected low - risk women ; however , further study is needed to confirm the safety of this approach . - pubmed
We set the conditions where CEF - PSII functioned or did not , by adding nigericin to the reaction mixture for the dissipation of DeltapH across thylakoid membranes , and then the thylakoids were illuminated . - pubmed
These results imply that CEF - PSII contributes to the protection of PSII from its photoinhibition with LEF , as an electron sink . - pubmed
, imatinib mesylate or interferon - alpha ) entails the risk of the development of a sudden blastic phase , which may not permit enough time to perform a SCT . - pubmed
IL-6 levels appeared to correlate positively with bilirubin and gamma - GT levels and negatively with the degree of acidosis . - pubmed
UNSPECIFIC STAINING OF PARAPROTEINS BY PICRIC ACID . - pubmed
In this study , we evaluated the role of Ras in Ag - induced airway inflammation and hyperresponsiveness by i.p . - pubmed
OVA challenge also caused airway hyperresponsiveness to methacholine , which was dose dependently blocked by treatment with TAT - dnRas . - pubmed
TAT - dnRas also blocked Ag - induced IL-4 and IL-5 , but not IFN - gamma , production in lung tissue . - pubmed
The latter include lecithinase positive Clostridium , citrate assimilating enterobacteria , as well as Escherichia with low biochemical activity and capacity for hemolysin production . - pubmed
The cytokine response profiles as determined by [ ( 3 ) H ]- thymidine incorporation assay were : 2 - to-12 fold growth stimulation of MUTZ-11 by GM - CSF , IFN - alpha ( interferon ) , IFN - beta , IFN - gamma , IL-3 and SCF ( stem cell factor ) ; growth inhibition by TGF - beta1 ( transforming growth factor ) , TNF - alpha ( tumor necrosis factor ) and TNF - beta . - pubmed
The transcription intensity of LeFRO1 in roots is dependent on the iron status whereas it is constitutively expressed in leaves . - pubmed
These results indicate that LeFRO1 is required in roots and shoots as well as in reproductive organs for iron homeostasis and that its transcription in roots and shoots is regulated by different control mechanisms . - pubmed
Acute administration of gamma - aminobutyric acid B ( GABAB ) receptor agonists decreased nicotine , cocaine , ethanol , and heroin self - administration . - pubmed
The present study investigated the effects of repeated subcutaneous administration of the GABAB receptor agonist CGP44532 on nicotine- and food - maintained responding under a fixed ratio 5 schedule of reinforcement . - pubmed
Two PPARgamma ligands , 15 - deoxy - delta12,14 - prostaglandin J2 ( PGJ2 ) and rosiglitazone , inhibited growth of all cell lines , with PGJ , being the most potent agent . - pubmed
PGJ2 , but not rosiglitazone , induced arrest of the cells in the G2 / M phase as well as apoptosis . - pubmed
CYP2C8 and CYP3A4 are the major enzymes responsible for the metabolism of cerivastatin , and a transporter , OATP2 , contributes to uptake of cerivastatin to the liver . - pubmed
To our knowledge , this is the first report showing that the adverse effect of cerivastatin might be caused by the genetic variant of CYP2C8 . - pubmed
Using RT - PCR , we determined the expression of angiogenic regulators in DMBA - induced intraductal hyperplasia ( IDP ) , carcinoma in situ ( CIS ) , invasive tumors ( INV ) , as well as normal tissue . - pubmed
Inhibition of IGF-1R by the tyrphostin , AG1024 , blocked endothelial tubulogenesis in vitro , confirming that IGF-1 functions as a regulator of angiogenesis . - pubmed
Lipid surface density and the surface structure of multilamellar ( MLVs ) and extruded unilamellar ( LUVs ) vesicles deposited on three types of polystyrene ( PS ) well - plates were probed by fluorescence and atomic force microscopy ( AFM ) imaging . - pubmed
Pharmacological inhibition of calcineurin , by the immunosuppressive drugs cyclosporin A and FK506 , affects a wide variety of diseases . - pubmed
The thermal TMS peroxide conversions documented provided biomimetic models for enzymatic conversions of fatty acid hydroperoxides and also offered a method to generate an array of oxylipin derivatives of value as reference compounds in GC - MS studies . - pubmed
Comparing the wild - type strain , which is positive in beta - glucuronidase activity , with the mutated strains named clone1 and clone2 in laboratory conditions , we observed that beta - glucuronidase influences the production of hyaluronic acid considerably and the molecular weight of hyaluronan slightly . - pubmed
The significant increase in the production of hyaluronic acid and the slight increase in the molecular weight are probably caused by an absence of free beta - glucuronic acid , due to its removal from the non - reducing termini of the polysaccharide by beta - glucuronidase . - pubmed
Association of amino acid variants in the activating transcription factor 6 gene ( ATF6 ) on 1q21 - q23 with type 2 diabetes in Pima Indians . - pubmed
Although the associations with type 2 diabetes and plasma insulin levels are marginal and their functional consequences are yet unknown , all three amino acid substitutions are located in a functionally important part of ATF6 . - pubmed
12 - Lipoxygenase utilizes arachidonic acid to synthesize 12 ( S )- hydroperoxyeicosatetraenoic acid , which is converted to the end product 12 ( S )- hydroxyeicosatetraenoic acid , an eicosanoid that promotes tumorigenesis and metastasis . - pubmed
The higher concentrations of SNP up - regulated the apoptosis - related protein expression , which is consistent with the analyses of sub - G(1 ) phase arrest , annexin V - FITC ( fluorescein isothiocynate ) staining , nuclear staining , and DNA fragmentation . - pubmed
Improvement of pulmonary function with imatinib mesylate in bronchiolitis obliterans following allogeneic hematopoietic cell transplantation . - pubmed
Recent studies suggest that beta - escin may possess anti - inflammatory , anti - hyaluronidase , and anti - histamine properties . - pubmed
Results also indicate that beta - escin inhibited growth of colon cancer cells with either wild - type or mutant p53 . - pubmed
This chemistry mimics that known for the copper enzyme dopamine - beta - monooxygenase . - pubmed
Design of antisense oligonucleotides and short interfering RNA duplexes ( siRNA ) targeted to BCL6 mRNA : towards rational drug development for specific lymphoma subsets . - pubmed
GTPase activating proteins ( GAPs ) help the GTPase return to the inactive GDP - bound form . - pubmed
GxcDD can bind to several Rac GTPases , but its function as a nucleotide exchange factor needs to be studied further . - pubmed
Aromatic compounds were UV irradiated ( 254 nm , 15 W ) to generate hydrogen peroxide , which was determined via PO - CL detection using a mixture of bis ( 2,4,6 - trichlorophenyl ) oxalate ( aryloxalate ) and 2,4,6,8 - tetrathiomorpholinopyrimido [ 5,4 - d ] pyrimidine ( fluorophore ) as a post - column CL reagent . - pubmed
Generation of hydrogen peroxide from aromatic compounds was confirmed using a flow injection analysis ( FIA ) system incorporating an enzyme column reactor immobilized with catalase . - pubmed
Dipeptide derivatives bearing various P2 residues and pyrrolidine derivatives as P1 mimics were evaluated in order to identify lead structures for the development of DPP8 and DPP9 inhibitors . - pubmed
After release of the SMA clamp , AM ( 12 mug / kg BW ) and AMBP-1 ( 40 mug / kg BW ) in combination or vehicle ( 1 - ml normal saline ) were administered intravenously over a period of 30 min . - pubmed
The mucosal barrier function in the small intestine was assessed in an isolated everted ileum sac using fluorescein - isothiocyanate dextran ( FD4 ) at 4 h after AM / AMBP-1 treatment . - pubmed
AIMS : There is no cure for pulmonary arterial hypertension , but current approved treatment options include prostanoids , endothelin - receptor antagonists , and phosphodiesterase type-5 inhibitors . - pubmed
Multiplex ligation - dependent probe amplification screening of isolated increased HbF levels revealed three cases of novel rearrangements / deletions in the beta - globin gene cluster . - pubmed
We selected 15 individuals with elevated fetal haemoglobin ( HbF ) levels ( & gt;5 % ) from a previous screening of 27 006 Korean individuals and analysed dosage changes of the globin gene cluster using multiplex ligation - dependent probe amplification ( MLPA ) . - pubmed
Three subjects were found to have deletions in the globin gene cluster , including a beta - thalassaemia due to deletion of HBB ( beta - globin gene ) , an HPFH due to deletions of HBD ( delta - globin gene ) and HBB , and an HPFH due to a novel HBG2 - HBG1 fusion gene consisting of exons 1 and 2 of HBG2 ( ( G)gamma - globin gene ) and exon 3 of HBG1 ( ( A)gamma - globin gene ) . - pubmed
The protein was crystallized using di [ poly ( ethylene glycol ) ] adipate , which also served as a cryoprotectant . - pubmed
The CB sections were prepared using the sodium alginate method and subjected to HE , PAS - AB and immunohistochemical stains . - pubmed
Here we report the discovery of newly designed and synthesized pyridylpyrimidine based potent and selective inhibitors of HIF-1alpha . - pubmed
Although dyslipidemia is the most influential and least treated factor , the greatest benefit for CVD prevention is obtained with integrated intervention on all risk factors , reducing blood pressure to below 140/80 mmHg , low - density lipoprotein cholesterol ( LDL - c ) to below 100 mg / dl , encouraging smoking cessation , regular physical activity and maintaining a healthy weight ( BMI & lt ; 25 kg / m2 ) . - pubmed
Here , we used single molecule force spectroscopy techniques to directly determine the mechanical strength of such highly covalent ferric - thiolate bonds in rubredoxin . - pubmed
Release was evoked by either veratridine ( 1 , 3.2 , or 10 muM ) , which prevents activated voltage - dependent Na (+ ) channels from closing , or elevation of extracellular [ K (+ ) ] from 3 to 15 mM. - pubmed
The exocytosis inhibitor tetanus toxin ( TeT ) or withdrawal of buffer Ca ( 2 + ) ( Ca ( e ) ( 2 + ) ) reduced K(+)-evoked release in both species , while blockade of Na (+ ) channels with tetrodotoxin had no effect . - pubmed
In addition , oxidative damage in the vessel wall was determined by measuring malondialdehyde concentrations , vascular superoxide production and SOD activity . - pubmed
The exaggerated vascular contractions were inhibited by both apocynin ( a Nox inhibitor ) and tempol ( a SOD mimetic ) in a concentration - dependent manner . - pubmed
The nicotine treatment significantly decreased the superoxide dismutase activity and increased malondialdehyde , superoxide and nitrotyrosine protein levels in the vascular wall . - pubmed
Consistently , antenatal nicotine exposure significantly enhanced the protein expression of NADPH oxidase Nox2 / gp91 , but not Nox4 in the aorta . - pubmed
CONCLUSIONS AND IMPLICATIONS : The present findings suggest that antenatal nicotine exposure results in the programming of heightened oxidative stress and vascular hypertensive reactivity via a Nox2 - dependent mechanism , leading to an increased risk of hypertension in adult offspring . - pubmed
This relative decrease in activity could be mainly related to increases in islet asymmetric dimethyl - arginine content , an endogenous inhibitor of nNOS activity . - pubmed
Furthermore , 17AAG blocked the interaction between Hsp90 and TGF - beta type II receptor ( TbetaRII ) and promoted ubiquitination of TbetaRII , leading to the decreased availability of TbetaRII . - pubmed
Smurf2 - specific siRNA reversed the ability of 17AAG to inhibit TGF - beta1 signaling . - pubmed
The effect of 17AAG on TbetaRII expression and renal fibrosis was confirmed in UUO kidneys . - pubmed
METHODS : Sinus grafts were performed by using granules of a HA / TTCP blend and a collagen membrane . - pubmed
Apocynin , manganese ( III ) tetrakis ( 1 - methyl-4 - pyridyl ) porphyrin , catalase , and diethyldithiocarbamate inhibited endothelium - dependent contractions . - pubmed
Biosimilars of filgrastim in autologous stem cell transplant : reduction in granulocyte - colony stimulating factor costs , but similar effects on bone marrow recovery . - pubmed
These results indicate that the CA1 dopamine D1 receptors may potentially play an important role in scopolamine - induced amnesia as well as the scopolamine state - dependent memory . - pubmed
Furthermore , our results propose that dopamine D1 receptor agonist , SKF38393 reverses the scopolamine - induced amnesia via acetylcholine release and possibly through the activation of muscarinic receptors . - pubmed
Alpha-1 - adrenergic receptors in heart failure : the adaptive arm of the cardiac response to chronic catecholamine stimulation . - pubmed
Alpha-1 - adrenergic receptors ( ARs ) are G protein - coupled receptors activated by catecholamines . - pubmed
There are far fewer alpha-1 - ARs than beta - ARs in the nonfailing heart , but their abundance is maintained or increased in the setting of heart failure , which is characterized by pronounced chronic elevation of catecholamines and beta - AR dysfunction . - pubmed
Seawater chromium , copper , manganese , nickel and zinc concentrations were measured , as well as metal accumulation , lipid peroxidation , protein oxidation , reduced glutathione levels , and enzymatic activities of superoxide dismutase and glutathione - S - transferase in gills and digestive glands . - pubmed
The main components of this disulfide relay machinery are the oxidoreductase Mia40 and the sulfhydryl oxidase Erv1 / ALR . - pubmed
INTERVENTION(S ): Goserelin administered on day 13 of life , CTX on day 18 of life , euthanasia on day 20 ( prepubertal ) , 56 ( pubertal ) , or 92 of life ( mature ) , measurements of body weight , length , uterine weight , serum antimullerian hormone and follicle - stimulating hormone , and histologic assessment of ovarian follicles and femur growth , and apoptotic rates by TUNEL . - pubmed
The nerve regeneration was systematically characterized by sciatic function index ( SFI ) , histological assessment including HE staining , immunohistochemistry , ammonia silver staining , Masson 's trichrome staining and TEM observation . - pubmed
Novel CT techniques , including adenosine stress CTP and TAG320 , may improve the diagnostic accuracy of CTA . - pubmed
Following on the success of Aripiprazole with its high clinical efficacy and minimal side effects , further antipsychotic drugs ( such as Bifeprunox ) have been developed based on the same dopamine D2 partial agonist pharmacological profile as Aripiprazole . - pubmed
The analysis was performed on a column of ultimate C18 ( 50 mm x 4.6 mm , 5 microm ) with the mobile phase consisting of methyl alcohol - water - formic acid = 75:25 : 0.05 at a flow rate of 0 . - pubmed
The areal density of Cu in CA1 decreased with the cumulative ( totaled over 21 stimulation days ) intensity and duration of electroshock - induced tonic seizures while Zn level in the hilus of DG was positively correlated with the duration and intensity of both tonic and clonic seizures . - pubmed
Exosomes enhanced ubiquitination of cellular proteins , including CD36 , and blockade of proteosome proteolysis with MG-132 rescued CD36 expression . - pubmed
The summary statistics indicated that calcium dobesilate was significantly associated with improving retinal microaneurysms ( RR : 0.62 , 95%CI : 0.42 - 0.90 , P=0.01 ) , retinal hemorrhages ( RR : 0.39 , 95 % CI : 0.17 - 0.88 , P=0.02 ) ; exudates ( RR : 0.31 , 95 % CI : 0.12 - 0.81 , P=0.02 ) , reduction of whole blood viscosity ( MD : -0.57 CP , 95 % CI : -0.75 to -0.38 , P<0.001 ) , plasma viscosity ( MD : -0.36 CP , 95 % CI : -0.63 to -0.09 , P=0.01 ) and blood cholesterol ( MD : -0.48 mg mL(-1 ) , 95 % CI : -0.64 - 0.33 , P<0.00001 ) . - pubmed
The other metabolites of cosmeceutical , medicinal , nutraceutical and industrial significance such as alpha - tocopherol , n - methylpyrrolidone ( NMP ) , trans - farnesol , prostaglandin F2 , protocatechuic , gallic and cinnamic acids were identified from non - aqueous extracts using GC - MS . - pubmed
omega-3FFA and tamoxifen significantly increased Erk1 / 2 and Akt phosphorylation levels in a dose and time dependent manner . - pubmed
Compared to omega-3FFA alone , the combination of tamoxifen with omega-3FFA significantly increased Erk1 / 2 and Akt phosphorylation levels . - pubmed
Protein instability in supercritical CO2 limits the application of this green solvent in enzyme - catalyzed reactions . - pubmed
Molecular dynamics simulation is used to monitor the effects of adding different natural osmolytes on the conformation and dynamics of chymotrypsin inhibitor 2 ( CI2 ) in supercritical CO2 . - pubmed
The NAD(+)-dependent deacetylase Sirt1 has been reported to play positive roles in the regulation of cellular homeostasis in response to various stresses . - pubmed
Although the copper - catalyzed azide - alkyne cycloaddition reaction efficiently introduced 237 copies of GM2 per Qbeta , this construct failed to induce significant amounts of anti - GM2 antibodies compared to the Qbeta control . - pubmed
In contrast , GM2 immobilized on Qbeta through a thiourea linker elicited high titers of IgG antibodies that recognized GM2 - positive tumor cells and effectively induced cell lysis through complement - mediated cytotoxicity . - pubmed
Cystathionine beta synthase ( CBS ) is a key enzyme in the methionine and cysteine metabolic pathway , acting as a metabolic gatekeeper to regulate the flow of fixed sulfur from methionine to cysteine . - pubmed
Mutations in the CBS gene cause clinical CBS deficiency , a disease characterized by elevated plasma total homocysteine ( tHcy ) and methionine and decreased plasma cysteine . - pubmed
The results suggest that the altered pharmacokinetics of DIC might be attributed to RSV mediated inhibition of CYP2C9 enzyme . - pubmed
AIM : The study aimed to determine whether Coca - Cola ( Coke ) Zero is a safe and effective solvent for polyethylene glycol ( PEG ) . - pubmed
Tryptophan Fluorescence Changes Related to Ca ( 2 +)- ATPase Function . - pubmed
We demonstrate here with Ca ( 2 + ) and two specific inhibitors of SERCA how this can be done by measurements of the intrinsic fluorescence of the tryptophan residues of SERCA . - pubmed
Optimum coagulant for coagulation for DON and TDN removals was characterized by the dense convex - concave packing structure differing from other zinc - based coagulant , polycrystalline structure and high content colloidal species , which could account up to 87 % of the total colloidal species . - pubmed
DON removal efficiency was also higher than and linearly correlated with total dissolved nitrogen ( TDN ) removal , which implies that an effective coagulation for TDN is also effective for DON . - pubmed
The electron donation to PSI was substantially reduced in iron - deficient cells . - pubmed
This decreased electron transfer may induce the over - reduction of the plastoquinone pool and consequently the appearance of acceptor side photoinhibition in PSII even at low light intensities . - pubmed
[ Effects of laser solid forming of porous titanium on proliferation of osteoblast and RANKL / OPG expression ] . - pubmed
OBJECTIVE : To evaluate the effect of laser solid forming ( LSF ) of porous titanium on receptor activator of NF - kappaB ligand ( RANKL)/osteoprorotegerin ( OPG ) expression and osteoblast cells growth . - pubmed
An Oxidoreductase AioE is Responsible for Bacterial Arsenite Oxidation and Resistance . - pubmed
In this study , we found that aioE is induced by AsIII and located in the arsenic island near the AsIII oxidase genes aioBA and co - transcripted with the arsenic resistant genes arsR1 - arsC1 - arsC2 - acr3 - 1 . - pubmed
AioE belongs to TrkA family corresponding the electron transport function with the generation of NADH and H + . - pubmed
An aioE in - frame deletion strain showed a null AsIII oxidation and a reduced AsIII resistance , while a cytC mutant only reduced AsIII oxidation efficiency . - pubmed
With AsIII , aioE was directly related to the increase of NADH , while cytC was essential for ATP generation . - pubmed
In addition , cyclic voltammetry analysis showed that the redox potential ( ORP ) of AioBA and AioE were +0.297 mV vs. NHE and +0.255 mV vs. NHE , respectively . - pubmed
The ORP gradient is AioBA & gt ; AioE & gt ; CytC ( +0.217 ~ +0.251 mV vs. NHE ) , which infers that electron may transfer from AioBA to CytC via AioE. - pubmed
The results indicate that AioE may act as a novel AsIII oxidation electron transporter associated with NADH generation . - pubmed
Since AsIII oxidation contributes AsIII detoxification , the essential of AioE for AsIII resistance is also reasonable . - pubmed
In this study , we found that it is possible to confer antimicrobial activity to Au NPs through precise control of their size down to NC dimension ( typically less than 2 nm ) . - pubmed
The peroxidase mimics , the CeO2 nanoparticles , as signal generators were integrated with the detection antibody . - pubmed
BACKGROUND : Interest in technetium-99 m ( 99mTc )- sestamibi imaging for neoadjuvant chemotherapy ( NAC ) response monitoring in locally advanced breast cancer ( LABC ) is increasing but remains matter of discussion . - pubmed
Resistance to the integrase strand transfer inhibitors raltegravir and elvitegravir is often due to well - identified mutations in the integrase gene . - pubmed
Furthermore , most in vitro experiments to select resistance to dolutegravir have resulted in few mutations of the integrase gene . - pubmed
Mutations selected in vitro by dolutegravir , located outside the integrase gene , can confer a high level of resistance to all integrase inhibitors . - pubmed
Most mutations selected by raltegravir , elvitegravir , or dolutegravir exposure are located inside the catalytic site of the integrase gene , but mutations outside the catalytic site of the integrase gene have also been selected with dolutegravir . - pubmed
Following in vitro selection with dolutegravir , we report , for the first time , a virus with selected mutations outside the HIV-1 integrase gene that confer resistance to all integrase inhibitors currently used to treat patients , such as raltegravir , elvitegravir , and dolutegravir . - pubmed
Our observation may explain why some viruses responsible for virological failure in patients treated with dolutegravir did not show mutations in the integrase gene . - pubmed
This trial compared dalcetrapib , the cholesteryl ester transfer protein inhibitor , with placebo in patients with recent ACS and was performed between April 2008 and September 2012 at 935 sites in 27 countries . - pubmed
There was broad application of evidence - based secondary prevention strategies after ACS , including use of statins in 4030 patients ( 97 % ) . - pubmed
Conclusions and Relevance : For patients with recent ACS who are treated with statins , Lp ( a ) concentration was not associated with adverse cardiovascular outcomes . - pubmed
BACKGROUND : A novel auto - cross - linked hyaluronic acid formulation ( HA - ACP ) has been proposed as a treatment for skin rejuvenation . - pubmed
Characterization of Melanin Radicals in Paraffin - embedded Malignant Melanoma and Nevus Pigmentosus Using X - band EPR and EPR Imaging . - pubmed
The EPR results revealed that the peak - to - peak linewidths ( DeltaHpp ) of paraffin - embedded MM and NP samples were 0.65 + - 0.01 and 0.69 + - 0.01 mT , respectively . - pubmed
Here , we performed replica exchange molecular dynamics simulations of Abeta1 - 42 in explicit water to study , at the molecular level , the effect of CLR01 binding to the lysine and arginine residues in the dimer . - pubmed
We found that CLR01 molecules encapsulate both lysine residues of each Abeta1 - 42 monomer while only establishing labile interactions with the arginine residues . - pubmed
To develop a safe SPE for energy storage applications , mechanically robust cross - linked epoxy matrix is combined with fast ion - diffusing ionic liquid / lithium salt electrolyte ( ILE ) via a simple one - pot curing process . - pubmed
The following type 2 inflammation markers were assessed : exhaled nitric oxide ( FeNO ) , serum periostin , serum eosinophil cationic protein ( S - ECP ) , and urinary eosinophil - derived neurotoxin ( U - EDN ) . - pubmed
RESULTS : Elevated U - EDN ( values in the highest tertile , & gt;=65.95 mg / mol creatinine ) was more common in subjects with fixed - AO vs. subjects without fixed - AO : 55 % vs. 29 % , P & lt ; 0.001 . - pubmed
Elevated U - EDN related to increased likelihood of having fixed - AO in both all subjects and never - smoking subjects , with adjusted ( adjusted for sex , age group , use of inhaled corticosteroids last week , atopy , early - onset asthma , smoking history , and packyears ) odds ratios ( aOR ) of 2.38 ( 1.28 - 4.41 ) and 2.51 ( 1.04 - 6.07 ) , respectively . - pubmed
We previously synthesized a novel biogenic nanoselenium ( BNS ) particles and proved that BNS could effectively improve intestinal antioxidative function through activating Nrf2 - ARE pathway . - pubmed
BNS particles significantly increased the content of phosphorylated - Nrf2 , without evident influence on the level of Kelch - like ECH - associated protein 1 ( Keap1 ) . - pubmed
Moreover , BNS also induced the activation of p38 , extracellular signal - regulated kinase 1 / 2 ( ERK1 / 2 ) , c - Jun N - terminal kinase , and AKT while phosphorylating Nrf2 . - pubmed
Overall , our data demonstrated that BNS particles activated Nrf2 - ARE pathway through p38 , ERK1 / 2 , and AKT mediated - phosphorylation of Nrf2 to improve the antioxidant function of intestinal epithelial cells . - pubmed
This study aimed to develop a novel dressing material for DS containing the phenolic agent guaiacol and evaluate its biological properties . - pubmed
Protein kinase C ( PKC ) is a class of phospholipid - dependent serine / threonine kinases that contribute to cell survival , migration , and invasion . - pubmed
However , the role of PKC in glucose - stimulated insulin secretion ( GSIS ) remains unclear . - pubmed
Finally , we showed that TRPC1 could reverse the decrease in intracellular Ca2 + levels and reduced insulin secretion induced by treatment with PKCalpha inhibitor under high glucose conditions . - pubmed
Nucleotide - binding oligomerization domain , leucine rich repeat , and pyrin domain - containing protein 3 ( NLRP3 ) inflammasome has been implicated in a series of physiological and pathological processes . - pubmed
Interference with rosuvastatin in vitro revealed that the expression of NLRP3 inflammasome and its downstream cytokines were significantly downregulated in both SAP and AMI groups in a time - dependent manner . - pubmed
The activation of NLRP3 inflammasome may be involved in the development of CHD , and rosuvastatin could attenuate the inflammatory process of atherosclerosis by downregulating NLRP3 expression and its downstream mediators . - pubmed
These findings indicated a potential role of NLRP3 in the pathogenesis and management of CHD , and also provided new insights into the mechanistic framework of rosuvastatin activity . - pubmed
A DNA radar was first built by assigning the silver nanocluster ( AgNC ) as the radar transmitter , the middle single strand DNA - bridge connected on the AgNCs as the electromagnetic wave , and the G4 / crystal violet complex as the radar antenna . - pubmed
Here , G4 is chosen as the suitable template to connect potential nanomaterial AgNCs with the G4 binder ( crystal violet , CV ) since the rich guanine in G4 could not only enhance the fluorescence of AgNCs but also form quartets offering powerful binding sites for the G4 binder . - pubmed
Revealing Lectin - sugar Interactions with a Single Au@Ag Nanocube . - pubmed
An individual nanoparticle - based plasmonic nanotechnology was used for real - time revealing lectin - sugar interactions , which could be designed as novel plasmonic nanobiosensors for detection of trace ConA with high sensitivity and selectivity . - pubmed
In fact , each Au@Ag NC can act as a nanobiosensor for quantified detection of trace ConA , which miniaturized the biosensor system to nanoscale . - pubmed
We expect that this nanobiosensor system can provide alternative important method for the monitoring the lectin - sugar specific binding at a single nanoparticle surface . - pubmed
Treatment with benzodiazepines , several previous psychiatric admissions , and the absence of improvement after ECT were associated with relapse . - pubmed
Peripheral ALCSF was statistically lower for both addition powers of the MFCLs when compared to SVCLs ( 12.70 for Add2 and Add4 , 13.73 for SVCLs , p = 0.009 ) . - pubmed
INTRODUCTION : Once - weekly semaglutide has been associated with greater reductions in glycated hemoglobin ( HbA1c ) and body weight than sitagliptin and dulaglutide in the SUSTAIN 2 and 7 clinical trials , respectively . - pubmed
IL-18 expression , cell proliferation , and apoptosis were examined using Western blotting , methylthiazolyldiphenyl - tetrazolium bromide assay , and flow cytometry , respectively . - pubmed
Further experiments to clarify the MOF 589 activity indicated that the BIPA - TC linker might have an important impact through a cooperative effect on the metal cluster . - pubmed
KEGG enrichment analysis found that cortisol regulated LPS - stimulated immune responses mainly focus on cytokines , complement and coagulation cascades pathways , antigen presentation pathways , haematopoiesis , and inflammation . - pubmed
METHODS : Twenty - eight well - trained swimmers completed an incremental test for maximal oxygen uptake ( Peak-[Formula : see text ] ) and maximal aerobic velocity ( MAV ) assessment . - pubmed
LncRNA HOTAIR - mediated MTHFR methylation inhibits 5 - fluorouracil sensitivity in esophageal cancer cells . - pubmed
Interference with lncRNA HOTAIR enhanced 5 - FU - induced apoptosis , exhibited anti - proliferative activity , and reduced promoter methylation of the MTHFR in EC cells . - pubmed
CONCLUSION : Together , our study proposes that pharmacologic targeting of lncRNA HOTAIR sensitizes EC cells to 5 - FU - based chemotherapy by attenuating the promoter hypermethylation of the MTHFR in EC . - pubmed
It was determined that the detection power of GC - MS system was improved 7.8 times for bixafen and 119 times for fipronil over LOD comparisons of conventional GC - MS and B - DLLME - GC - MS systems . - pubmed
Meanwhile , development of therapeutic resistance to trastuzumab , a common breast cancer treatment administered after chemotherapy , is a significant challenge in the treatment of HER2 - positive breast cancer . - pubmed
We , therefore , analyzed the role of YAP in trastuzumab resistance in HER2 - positive - breast carcinoma cells in vitro and evaluated the status of YAP and related proteins in patient - derived breast carcinoma tissues by immunohistochemistry . - pubmed
In both paclitaxel , doxorubicin and cyclophosphamide ( TAC)-treated , and docetaxel , carboplatin , and trastuzumab ( TCbH)-treated groups ; the pathological complete response ( pCR ) ratios were inversely correlated with p - AKT status in biopsy specimens , while YAP and p73 status were positively correlated with the pCR ratio in the biopsy specimens of the TCbH group . - pubmed
Curcumol inhibits KLF5 - dependent angiogenesis by blocking the ROS / ERK signaling in liver sinusoidal endothelial cells . - pubmed
Interestingly , this process may depend on curcumol 's inhibition of the expression of transcription factor KLF5 . - pubmed
More importantly , we proved that curcumol could suppress KLF5 - mediated LSEC angiogenesis by inhibiting ROS / ERK signaling . - pubmed
SIGNIFICANCE : We suggested that transcription factor KLF5 could be considered as a new target molecule of curcumol in improving liver fibrosis , and pointed out that curcumol targeted ROS / ERK - mediated KLF5 expression could inhibit LSEC angiogenesis . - pubmed
In this prospective trial , a pan - genotypic PrEP with daclatasvir and sofosbuvir once daily for 12 week was administered at transplantation . - pubmed
Moreover , the alkaline phosphatase ( ALP ) activity at day 7 and the semiquantitative analysis of Alizarin red staining at day 21 demonstrated that osteogenic differentiation of hBMSCs was enhanced in the Ta group . - pubmed
Here , we report a highly efficient catalyst in acid , that is , solid - solution Ru - Ir nanosized - coral ( RuIr - NC ) consisting of 3 nm - thick sheets with only 6 at.% Ir . - pubmed
Among OER catalysts , RuIr - NC shows the highest intrinsic activity and stability . - pubmed
In addition to an anaerobic environment , maximum enzyme synthesis was dependent upon the presence of a complete complement of amino acids , with omission of L - threonine , L - valine , or L - leucine producing the largest decreases in enzyme formation . - pubmed
Half - maximal stimulation of enzyme synthesis occurred with 0.4 mM threonine in the medium . - pubmed
The roles of anaerobiosis and amino acids are interpreted as being in accord with the concept that threonine dehydratase functions in anaerobic energy production under conditions of amino acid sufficiency . - pubmed
In the present study , using flow cytometric analysis of the binding of merocyanine 540 to monitor transbilayer phospholipid distribution , Ca ( 2 +)-induced loss of asymmetry is shown to be independent from the aminophospholipid translocase which catalyzes movement of normally internal phospholipids from the outer to the inner leaflet of the membrane . - pubmed
Addition of ATP during lysis reverses loss of asymmetry , and this restoration can be blocked by inhibitors of the aminophospholipid translocase . - pubmed
The interplay of purified eIF-4A , - 4B , and - 4F was studied in relation to ATP dependence and cap analogue sensitivity of cap binding . - pubmed
The interaction of eIF-2 with the 5 ' end of mRNA was extremely sensitive to K(+)-ions and was resistant to a high concentration of Mg ( 2 +)-ions ; this influence of mono- and divalent ions was in contrast with the cross - linking of eIF-4B and - 4E. - pubmed
Effects of dopamine receptor antagonists on the inhibition of the spike generation by conditioning stimuli applied to the ventral tegmental area preceding the test stimulus to the parafascicular nucleus and those of dopamine agonists on the test stimulus - induced spikes were examined . - pubmed
For the rest of the day till the following morning after the test the blood sugar was elevated , as compared with the control level of the 4th day . - pubmed
Neutrophil elastase activity and superoxide production are diminished in neutrophils of alcoholics . - pubmed
The aim of this study was to examine the effect of ethanol consumption on neutrophil elastase activity and superoxide production of peripheral blood neutrophils . - pubmed
After 6 to 10 days , elastase activity and superoxide production were significantly greater than they were at Day 0 and approached normal levels . - pubmed
However , the type IIIb enzyme has a Ki for trimethoprim of 0.4 microM , and a pI of 5.35 ( as compared to 19 nM and 6.1 for the type IIIa ) ; both these results suggest that it is a different enzyme from the prototype type IIIa . - pubmed
The type IIIb dihydrofolate reductase was purified by methotrexate agarose affinity chromatography , yielding a pure protein as determined by HPLC . - pubmed
Automatic amino acid analysis of the purified enzyme showed it to be distinct from all other known plasmid - encoded dihydrofolate reductases and quite different from the type IIIa enzyme . - pubmed
The purified enzyme was examined by SDS - PAGE , which revealed that the type IIIb dihydrofolate reductase was a monomeric protein of Mr 17,200 . - pubmed
No isotope effect ( 1.00 + /- 0.03 ) was observed in the 5 - lipoxygenase reaction for conversion of the initially formed product 5 - hydroperoxyeicosatetraenoic acid to the epoxide leukotriene A4 . - pubmed
Correlation of some published amino acid sequences for photosystem I polypeptides to a 17 kDa LHCI pigment - protein and to subunits III and IV of the core complex . - pubmed
237 , 108 - 112 ] is not that of subunit IV but rather that of the 17 kDA LHCIc pigment protein . - pubmed
14 , 511 - 518 ] is indeed that of subunit III ; seemingly conflicting identifications , based on apparent sizes on SDS - PAGE , of which polypeptides are subunits III and IV are probably explained by subunit III 's electrophoretic migration rate being dependent on the solvent . - pubmed
The administration of DISO prior to overdrive atrial pacing improves the rate of conversion to SR and allows an easier stimulation protocol with a lower incidence of pacing - induced atrial fibrillation . - pubmed
Since it has been demonstrated that the use of opiates during pregnancy alters placental receptors , the effects of cocaine on placental receptors was studied . - pubmed
This study investigated the roles of cholinergic reflex stimulation and decreased beta 2 - adrenoceptor responsiveness to explain adenosine 's bronchoconstrictor action . - pubmed
The IB increased FEV1 by 11 to 15 % and SGaw by 69 to 73 % and provided a large degree of protection against methacholine , with a geometric mean concentration ratio ( CR ) of 196 when airway caliber was measured as SGaw ( p less than 0.001 ) . - pubmed
In contrast , IB provided little protection against adenosine - induced bronchoconstriction ( CR 1.3 for SGaw and 1.51 for FEV1 ) . - pubmed
Various calmodulin antagonists such as chlorpromazine , trifluoperazine and N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalenesulfonamide , not only activated the three reactions but also greatly enhanced their sensitivity to Ca2 + ( K0.5 , 0.1 - 0.3 microM ) . - pubmed
Although platelet membranes contained endogenous calmodulin ( 0.3 - 0.6 microgram / mg of membrane protein ) , the addition of exogenous calmodulin inhibited choline exchange activity but had no or little effect on serine or ethanolamine exchange activity . - pubmed
The results suggest that in the presence of low Ca2 + concentrations , these drugs markedly stimulate base - exchange activities , and choline exchange activity may be regulated by calmodulin . - pubmed
There was a significant fall in basal levels of gastrin ( -39 % , P less than 0.001 ) ; gastric inhibitory peptide ( -44.7 % , P less than 0.001 ) ; enteroglucagon ( -25 % , P less than 0.001 ) and neurotensin ( -33 % , P less than 0.05 ) . - pubmed
There was no significant change in plasma levels of insulin , motilin , pancreatic polypeptide or glucose . - pubmed
Specific immunoprecipitates of aminopeptidase M , an established transmembrane protein , and of gamma - glutamyltransferase were isolated from vesicles solubilized with Triton X-100 or with papain . - pubmed
With the samples derived from internally labeled vesicles , the triton - solubilized form of each enzyme was intensely labeled , whereas the papain - solubilized forms contained insignificant amounts of radioactivity . - pubmed
No gradient was recorded at rest , but it could be provoked by isoproterenol . - pubmed
The levels of all other amino acids in blood , urine , and CSF were within the normal range . - pubmed
Since aspartic acid is a putative excitatory neurotransmitter , a possible causal relationship is suggested between its increased CSF concentration and the occurrence of neonatal convulsions in this family . - pubmed
After introduction of screening for alanine aminotransferase and antibodies to hepatitis B core antigen the risk of seroconversion was 4 % per patient ( 0.26 % per unit ) . - pubmed
CONCLUSION : These results suggest that modification of the treatment regimen is needed , with higher doses of clarithromycin and combination therapy with proton - pump inhibition . - pubmed
RSC - b and - c were found to be homologous proteins having similar amino acid compositions and N - terminal sequences . - pubmed
These enzymes are stable at pH 4 - 8 and their optimum pHs toward glycolchitin are 5 . - pubmed
Superoxide dismutase activities and mRNA levels were not affected by hyperoxia , whereas catalase activity was slightly decreased after 24 h in 80 % oxygen at Day 17 . - pubmed
The presence of mGluR1 alpha in cells of the hippocampus that are associated with the release of somatostatin , suggest that this receptor could play a role in regulating hippocampal excitability in both normal and epileptic tissues . - pubmed
The evidence suggests that the clones contain DNA sequences encoding functions required for geldanamycin biosynthesis including components of the polyketide synthase . - pubmed
The activities of dephosphophosphorylase kinase and phosphorylase a were stimulated by each hormone or Bt2cAMP . - pubmed
Pretreatment of leaves with cycloheximide does not inhibit the response to auxin treatment , suggesting that hormone sensitivity of these promoter elements does not involve de novo synthesis of ASF-1 . - pubmed
Based on these results , we propose that transcriptional activation by ASF-1 may be modulated by auxin through modification of pre - existing factors . - pubmed
Our results also suggest a role for ASF-1 in mediating some of the effects of auxin in vivo . - pubmed
An increased sensitivity of glycolysis to insulin measured in vitro by both lactate production and glucose transport was observed following the acute exercise . - pubmed
This process was reversed by the addition of 2 - chloroadenosine , an adenosine receptor agonist , to the incubation medium . - pubmed
Our findings confirm earlier studies suggesting that improved insulin sensitivity of glucose utilization by skeletal muscles after endurance exercise may be at least partly explained by an alteration in the adenosine concentration or changes in the properties of adenosine receptors in soleus muscle . - pubmed
Prolactin enables normal development of ACh - stimulated current in cultured larval bullfrog skin . - pubmed
ACh , carbamylcholine , and choline stimulated SCC in skin that had been cultured with aldosterone ( 5 x 10(-7 ) M ) supplemented with prolactin ( PRL ; 2 micrograms / ml ) . - pubmed
Eserine , an inhibitor of acetylcholinesterase , also inhibited the ACh response . - pubmed
The main tumour results were : ( a ) original DS induced a higher tumour rate than extracted DS ; ( b ) the carcinogenic potency of extracted CB probably depends on the size of the primary carbon particles and on the specific surface area of the particles ; ( c ) extracted DS covered with 11 micrograms BaP per mg carbon particles caused a lower lung tumour rate than original DS containing only 0.9 ng BaP per mg , but a variety of other PAH and NO2 - PAH ; ( d ) a total dose of 15 mg pure BaP caused a lung tumour rate very similar to that of 30 mg extracted DS , 15 mg original DS or 15 mg Printex 90 T CB extracted or covered with approximately 29.5 micrograms BaP per mg CB . - pubmed
Addition of calmodulin ( 1 microM ) , or the calmodulin inhibitors mastoparan and calmidazolium ( 5 microM ) , did not alter the action of Ca2 + on the cGMP - activated current . - pubmed
We analysed fatty acid compositions , in particular arachidonate , and measured the amount and the activity of high molecular weight cytoplasmic phospholipase A2 ( cPLA2 ) of these tissues . - pubmed
cPLA2 was separated by SDS - PAGE , Western - blotted , immunoblotted using a specific antibody to cPLA2 and semiquantified following enhanced chemiluminescence using a scanning densitometer . - pubmed
cPLA2 biological activity was also assayed using 1 - stearoyl , 2 -[ 1 - 14C ]- arachidonyl , L-3 - phosphatidylcholine . - pubmed
Compared with normal mucosa / submucosa , there was more total arachidonate in tumours ( P & lt ; 0.01 ) , and increased levels of cPLA2 occurred in 6 of 17 tumours . - pubmed
There is a serine tRNA binding site downstream of the 5 ' LTR sequence and a polypurine tract upstream of the 3 ' LTR end . - pubmed
When the highly purified polyisoprenyl phosphate phosphatase was analyzed by SDS - polyacrylamide gel electrophoresis , a major 33 - kDa polypeptide was observed . - pubmed
Enzymatic dephosphorylation of Dol - P by the purified phosphatase was 1 ) optimal at pH 7 ; 2 ) potently inhibited by F- , orthovanadate , and Zn2 + & gt ; Co2 + & gt ; Mn2 + but unaffected by Mg2 + ; 3 ) exhibited an approximate Km for C95 - Dol - P of 45 microM ; and 4 ) was sensitive to N - ethylmaleimide , phenylglyoxal , and diethylpyrocarbonate . - pubmed
The pig brain phosphatase did not dephosphorylate glucose 6 - phosphate , mannose 6 - phosphate , 5 '- AMP , or p - nitrophenylphosphate , but it dephosphorylated dioleoyl - phosphatidic acid at initial rates similar to those determined for Dol - P. Based on the virtually identical sensitivity of Dol - P and phosphatidic acid dephosphorylation by the highly purified enzyme to N - ethylmaleimide , F - , phenylglyoxal , and diethylpyrocarbonate , both substrates appear to be hydrolyzed by a single enzyme with an apparent dual specificity . - pubmed
Although the enzyme is Mg2 + -independent and capable of dephosphorylating Dol - P and PA , several enzymological properties distinguish this lipid phosphomonoesterase from PAP2 . - pubmed
The production and release of pro - inflammatory cytokines are affected by theophylline showing a potent inhibitory effect on the production of IL-1beta , TNF - alpha and IFN - gamma . - pubmed
Nycthemeral significant variation in factor VII when acenocoumarol was administered once daily was noted ( P = 0.02 ) , but the clinical relevance of factor VII variation at steady - state is uncertain . - pubmed
The CCl4 administration alone caused hepatocellular necrosis , severe anemia , leucopaenia , lymphocytopaenia , neutrophilia , eosinophilia and haemoglobinaemia along with the alterations of plasma albumin and globulin . - pubmed
These cardiovascular effects appear to involve complex interactions at the level of the peripheral beta ( 1 )- , beta ( 2 )- , and alpha ( 2 )- adrenoreceptor - dependent as well as M ( 2 )- and M ( 4 )- cholinergic receptor - dependent transductional pathways both in cardiovascular myocells and at the level of the postganglionic sympathetic endings ( with reserpine- and guanethidine - like mechanisms ) . - pubmed
Thyroid peroxidase ( TPO ) as a tumor marker in the follow - up of differentiated thyroid carcinomas with surgical and ablative radioiodine therapy . - pubmed
In relation to the extension of residues , some cases presented increasing of TPO and hTG after radioiodine treatment . - pubmed
The clinical value of TPO seems to be in the time being limited to DTA due to false negative hTg values . - pubmed
An energy transfer acceptor was attached to Cys374 of actin and a donor to the tropomyosin heterodimer at either Cys36 of the beta - chain or Cys190 of the alpha - chain . - pubmed
ATP , which dissociates myosin from actin , completely reversed the changes in FRET induced by heads , whereas in the presence of ADP the effect of heads was the same as in its absence . - pubmed
The results indicate that myosin with and without ADP , intermediates in the myosin ATPase hydrolytic pathway , are effective regulators of tropomyosin position , which might play a role in the regulation of smooth muscle contraction . - pubmed
Five of the anti - CYP2D6 - positive sera recognized a peptide between amino acids 200 and 429 including amino acids 254 - 271 . - pubmed
Androgens mediate their effects through the androgen receptor ( AR ) and have antiproliferative effects on uterine endometrial cells . - pubmed
Reverse transcription - polymerase chain reaction was performed by using UEC cell lines with and without treatment by the demethylating reagent 5 - aza-2 '- deoxycytidine . - pubmed
Treatment with 5 - aza-2 '- deoxycytidine restored AR expression in all of the UEC cell lines that showed no AR expression before treatment . - pubmed
This study is the first to report that the possible mechanism of AR inactivation in endometrial cancer is through hypermethylation of the AR gene CpG islands . - pubmed
This hyperthermophilic enzyme did not show domain movement upon binding of an acidic substrate , glutamate , except for a small movement of the alpha - helix from Glu16 to Ala25 . - pubmed
Fluorescent staining of glycoproteins on polyvinylidene difluoride membrane with 8 - aminonaphthalene-1,3,6 - trisulfonate . - pubmed
Here , we describe a simple and sensitive method that allows fluorescent detection of glycoproteins on polyvinylidene difluoride ( PVDF ) membrane . - pubmed
We used periodic acid oxidation of carbohydrate chains of glycoproteins and fluorescent labeling with 8 - aminonaphthalene-1,3,6 - trisulfonate ( AN - TS ) by reductive amination . - pubmed
There is compelling evidence that topical corticosteroids down - regulate cytokine expression , and it is likely that a larger , and longer multi - centre trial may prove their efficacy in CRS . - pubmed
The detrimental effects that cytokines impart on metabolic function have been associated with nitric oxide ( NO ) production and apoptosis . - pubmed
RESULTS : Cytokine - induced suppression of glucose - stimulated insulin release was enhanced in a time - dependent manner . - pubmed
NO production was stimulated by IL-1 beta and augmented by TNFalpha and IFN gamma . - pubmed
N ( G )- Monomethyl - l - arginine ( l - NMMA ) blocked cytokine - induced NO production but only partially attenuated suppression of glucose - stimulated insulin release . - pubmed
Apoptosis increased in the presence of cytokines and was slightly reduced when NO production was specifically inhibited . - pubmed
Here , we extended this observation reporting that the mutation of serine-46 of Cdc28 to glutamic acid doubles , at least in vitro , the H1 - kinase activity of the Cdc28 / cyclin A complex . - pubmed
Corticosteroid hormone - induced factor ( CHIF ) is a short epithelial - specific protein that is independently induced by aldosterone and a high - K(+ ) diet . - pubmed
A number of studies have suggested that these proteins are involved in the regulation of ion transport and , in particular , functionally interact with the Na (+)- K (+)- ATPase . - pubmed
Upregulation of VEGF mRNA by hypoxia was mimicked by CoCl2 and desferrioxamine in normoxic AEC and was not prevented by inhibitors of reactive oxygen species , suggesting that hypoxic VEGF regulation involved an O2 - dependent protein that requires ferrous ions but is independent of reactive oxygen species generation . - pubmed
Cocaine - induced proliferation of dendritic spines in nucleus accumbens is dependent on the activity of cyclin - dependent kinase-5 . - pubmed
It has been shown previously that chronic cocaine administration increases the expression of cyclin - dependent kinase-5 in this brain region and that this neuronal protein kinase regulates cocaine - induced locomotor activity . - pubmed
These data indicate that cyclin - dependent kinase-5 is involved in regulation of , as well as cocaine - induced changes in , dendritic spine density . - pubmed
Mean arterial ( MAP ) and corpus cavernosal ( CCP ) pressures were monitored during intracavernosal injection of the Rho - kinase inhibitor Y-27632 . - pubmed
Intense neural activity reduced glycogen content in the presence of 10 mM bath glucose and CAP area gradually declined . - pubmed
CAP area declined more rapidly during high frequency stimulation if monocarboxylate transport was inhibited . - pubmed
The comparable decrease in activities was observed for the ouabain - sensitive and ouabain - resistant Na + , K (+)- ATPase components ( isoform species ) . - pubmed
Mg ( 2 +)- ATPase increases in postnatal period independent of Na + , K (+)- ATPase activity and it remains insensitive to postnatal maternal alcohol intake . - pubmed
The decomposition of hydrogen peroxide by hemocyanin and by its dissociation products . - pubmed
Sulfhydryl content and tryptic susceptibility of thermally denatured ovalbumin . - pubmed
[ Protein concentration , hemoglobin , hematocrit values and oxygen content in maternal and umbilical cord blood ] . - pubmed
The title 4,4 '- disubstituted diphenyl-1,3 - triazines , C14H15N3 , ( I ) , C12H9ClFN3 , ( II ) , and C13H12FN3 , ( III ) , each contain a triazene group ( - N = N - NH - ) having an extended conformation . - pubmed
The dihedral angles between the two benzene rings in ( I ) , ( II ) and ( III ) are 4.3 , 3.4 and 6.5 degrees , respectively . - pubmed
Nitric oxide ( NO * ) is an effective chain - breaking antioxidant in free radical - mediated lipid oxidation ( LPO ) . - pubmed
Using oxygen consumption as a measure of LPO , we simultaneously measured nitric oxide and oxygen concentrations with NO * and O2 electrodes . - pubmed
Bolus addition of NO * ( 1.5 microM ) inhibited LPO when the NO * concentration was greater than 50 nM. - pubmed
Similarly , using ( Z )- 1 -[ N -( 3 - ammoniopropyl )- N -( n - propyl ) amino ] diazen-1 - ium-1,2 - diolate as a NO * donor we found that an average steady - state NO * concentration of at least 72 + /- 9 nM was required to blunt LPO . - pubmed
Erlectin ( XTP3 - B ) is member of a protein family containing mannose 6 - phosphate receptor homology ( MRH- , or PRKCSH- ) domains implicated in N - glycan binding . - pubmed
Aldh null larvae and adults are poisoned by ethanol concentrations easily tolerated by wild - types ; their ethanol sensitivity is in fact comparable to that of Adh nulls . - pubmed
reinhardtii and the saturation of ribulose-1,5 - bisphosphate carboxylase at low extracellular carbon concentrations . - pubmed
Since translatable RNA for the polypeptide responsible for carbonic anhydrase activity was only present in cells that experienced low levels of CO ( 2 ) , the adaptation process either involves the regulation of transcription of the carbonic anhydrase gene ( and perhaps other genes involved in adaptation ) or the post - transcriptional processing of the messenger RNA . - pubmed
The isolates could be divided into six classes , namely beta - lactamase - non - producing ampicillin - susceptible ( 25.0 % ) , TEM-1 beta - lactamase - producing ampicillin - resistant ( 11.0 % ) , beta - lactamase - non - producing low - level ampicillin - resistant with N526 K or R517H substitution in the ftsI gene ( 30.4 % ) , BLNAR with an S385 T substitution together with either N526 K or R517H substitution in ftsI ( 22.2 % ) , BLPACR - I with either a N526 K or R517H substitution in ftsI ( 9.5 % ) and BLPACR - II with an S385 T substitution together with either a N526 K or R517H substitution in ftsI ( 1.9 % ) . - pubmed
Using CYP supersomes and liver microsomes , it was shown that aplidine was metabolised mainly by CYP3A4 and also by CYP2A6 , 2E1 and 4A11 . - pubmed
However , the aplidine metabolites formed by CYP were further conjugated by the phase II enzymes UGT , GST and SULT . - pubmed
These results provide evidence that CYP3A4 has a major role in metabolising aplidine in vitro with additional involvement of CYP2A6 , 2E1 , and 4A11 . - pubmed
CoCl ( 2 ) microinjected in the vMPFC significantly reduced freezing and attenuated MAP and HR increase of the conditioned group . - pubmed
However sulphur treatment is not without its risks ; a sulphur spring dermatitis has been described from a spa bath in Taiwan . - pubmed
Carbon - ion radiotherapy for locally advanced or unfavorably located choroidal melanoma : a Phase I / II dose - escalation study . - pubmed
Following the insult , synaptic activity was examined by recording orthodromically evoked CA1 subfield responses , while mitochondrial activity was assessed by spectrophotometric measurement of formazan produced by metabolism of 2,3,5 - triphenyltetrazolium chloride . - pubmed
Inhibition of the 26 S proteasome with MG132 rescued cyclin D1 protein levels , indicating that rather than inhibiting translation , PKR and PERK act to increase cyclin D1 degradation . - pubmed
Interestingly , this effect still requires eIF2alpha phosphorylation at serine 51 , as cyclin D1 remains unaffected in cells containing a non - phosphorylatable form of the protein . - pubmed
Furthermore , this degradation is independent of phosphorylation of cyclin D1 at threonine 286 , which is mediated by the glycogen synthase kinase 3beta and mitogen - activated protein kinase pathways as described in previous studies . - pubmed
These data show that the administration of epibatidine induces excitation of locus coeruleus neurons , which is mediated by nicotinic receptors . - pubmed
Identification of downstream targets of estrogen and c - myc in breast cancer cells . - pubmed
The lipase showed high preference for the S (+)- enantiomer of ibuprofen , which was esterified to the corresponding S (+)- ibuprofen ester . - pubmed
The enzyme activities of n - alcohols with different chain lengths ( 3 - 12 ) were compared , and it appeared that short- ( propanol and butanol ) and long - chained ( decanol and dodecanol ) alcohols were better substrates than the intermediate ones ( pentanol , hexanol , and octanol ) . - pubmed
However , unlike secondary and tertiary alcohols , all of the tested primary alcohols were substrates for the lipase . - pubmed
, the hydrolysis of racemic ibuprofen ester in the microemulsion ) was also carried out enantioselectively by the enzyme . - pubmed
The high enantioselectivity displayed by the lipase in the microemulsion system was seen neither in a similar esterification reaction in a pure organic solvent system ( isooctane ) nor in the hydrolysis reaction in an aqueous system ( buffer ) . - pubmed
The residues in FUS that are phosphorylated in vitro and in vivo were identified , and phospho - specific antibodies were generated to demonstrate that FUS becomes phosphorylated at Ser(42 ) in vivo , primarily in response to agents that cause DSBs ( double - strand breaks ) . - pubmed
DSB - induced FUS phosphorylation in vivo at Ser(42 ) requires ATM and not DNA - PK . - pubmed
Although Ser(42 ) is retained in the oncogenic FUS - CHOP [ C / EBP ( CCAAT / enhancer - binding protein )- homologous protein 10 ] fusion generated by a t(12;16)(q13;p11 ) chromosomal translocation , Ser(42 ) in FUS - CHOP is not phosphorylated after DNA damage . - pubmed
Post - translational modification of histone N - tails affects eukaryotic gene activity . - pubmed
The treatment of pertussis and pneumonia complicating pertussis ; the role of hyperimmune gamma globulin and sulfadiazine . - pubmed
Carbonyl Reductase 3 ( CBR3 ) Mediates 9 - cis - Retinoic Acid - Induced Cytostatis and is a Potential Prognostic Marker for Oral Malignancy . - pubmed
Our results suggest that the cytostatic effects of RA could be mediated by the activation of endogenous CBR3 gene in oral squamous cell carcinomas ( OSCCs ) , and the expression is a potential marker for oral malignancy . - pubmed
Melatonin transmits photoperiodic signals through the MT1 melatonin receptor . - pubmed
All data suggest that the melatonin signal that transmits photoperiodic information to the hypothalamo - hypophysial axis acts on the MT1 receptor . - pubmed
Activated pancreatic stellate cells ( PSC ) play a central role in the pathogenesis of pancreatic fibrosis , a common feature of chronic pancreatitis which is often caused by excessive alcohol consumption . - pubmed
In view of the central role of connective tissue growth factor ( CCN2 ) in fibrosis , we investigated the mechanisms by which CCN2 is regulated in PSC following their exposure to ethanol or acetaldehyde . - pubmed
CCN2 production , assessed by reverse - transcriptase polymerase chain reaction to measure CCN2 mRNA levels or by fluorescence activated cell sorting to assess CCN2 protein , was enhanced in a dose - dependent manner by ethanol or acetaldehyde . - pubmed
In the presence of 4MP , DAS , or the anti - oxidants vitamin D or catalase , there was a substantial decrease in the ability of ethanol to stimulate CCN2 mRNA expression and a concomitant decrease in CCN2 - positive PSC . - pubmed
Accumulation of reactive oxygen species in PSC after exposure to ethanol was verified by loading the cells with dichlorofluorescin diacetate and showing that there was a stimulation of its oxidized fluorescent product , the latter of which was diminished in the presence of catalase or vitamin D. - pubmed
The potential therapeutic effects of inhibitors of ethanol metabolism or anti - oxidants in alcoholic pancreatitis may arise in part through their ability to attenuate CCN2 production by PSC . - pubmed
The incretins and beta - cell health : contrasting glucose - dependent insulinotropic polypeptide and glucagon - like peptide-1 as a path to understand islet function in diabetes . - pubmed
None of the oils showed a hydroxyl radical scavenging capacity & gt ; 50 % , but they showed an ability to inhibit 5 - lipoxygenase . - pubmed
Pilot comparison of F - fluorocholine and F - fluorodeoxyglucose PET / CT with conventional imaging in prostate cancer . - pubmed
Positron emission tomography / computed tomography ( PET / CT ) with ( 18 ) F - flurodeoxyglucose ( FDG ) is also often suboptimal because of low tracer avidity , but ( 18 ) F - fluorocholine ( FCH ) appears to be a promising alternative molecular imaging probe . - pubmed
RESULTS : Based on all imaging - detected disease sites , both FCH - PET / CT and FDG - PET / CT ( 79 % ) were more sensitive than CI ( 14 % ) , with the highest number of sites of nodal and distant disease on FCH PET / CT . - pubmed
Neonicotinoid insecticides control crop pests based on their action as agonists at the insect nicotinic acetylcholine receptor , which accepts chloropyridinyl- and chlorothiazolyl - analogs almost equally well . - pubmed
These neonicotinoids effect a similar global transcriptional response to that of SA , including genes involved in ( a)biotic stress response . - pubmed
Furthermore , similar to SA , IMI and CLO induce systemic acquired resistance , resulting in reduced growth of a powdery mildew pathogen . - pubmed
The action of CLO induces the endogenous synthesis of SA via the SA biosynthetic enzyme ICS1 , with ICS1 required for CLO - induced accumulation of SA , expression of the SA marker PR1 , and fully enhanced resistance to powdery mildew . - pubmed
In contrast , the action of IMI does not induce endogenous synthesis of SA . - pubmed
Instead , IMI is further bioactivated to 6 - chloro-2 - hydroxypyridinyl-3 - carboxylic acid , which is shown here to be a potent inducer of PR1 and inhibitor of SA - sensitive enzymes . - pubmed
Thus , via different mechanisms , these chloropyridinyl- and chlorothiazolyl - neonicotinoids induce SA responses associated with enhanced stress tolerance . - pubmed
METHODS : PCR was applied to obtain Flavin - binding monooxygenase genes , then quantitative real - time polymerase chain reaction ( Q - RT - PCR ) and RT - PCR were applied to analyze gene expression in response to different LC - alkanes and pristane . - pubmed
Enhanced expression of almA1 genes was observed when strain A-11 - 3 grew with long chain alkanes ( C28 to C32 ) , in sodium acetate medium . - pubmed
Similarly , almA2 was induced by long chain alkanes ( C24 to C34 ) . - pubmed
CONCLUSION : AlmA genes were mostly responsible for the degradation of long - chain alkanes and pristane in strain A-11 - 3 . - pubmed
In addition , Thy-1 triggering enhanced cytokine secretion by iNKT cells that were concomitantly stimulated with alpha - galactosylceramide ( alphaGC ) , consistent with a co - stimulatory role for Thy-1 in iNKT cell activation . - pubmed
A 30 - mer phage displaying peptide library was constructed and screened against immobilized host cells , in which each peptide was overlapped 10aa to another peptide and spanned all amino acid sequences of Erns and E2 . - pubmed
Amino acid sequence analyses for two phage clones ( P2 and P6 ) demonstrated the strongest binding positions were at 101 - 130 ( S2 ) in Erns , and 141 - 170 ( S6 ) in E2 , respectively . - pubmed
The mechanisms by which thyroid stimulating hormone ( TSH ) regulates the expression and activity of sodium iodide symporter ( NIS ) through cAMP - PKA have been partially elucidated by many studies . - pubmed
In this study , we investigated the effects of the TSH - mediated cAMP - PKA and PLC - IP3 pathways on the expression of NIS in the presence of various intracellular iodinated compounds . - pubmed
A high concentration of iodine increased NIS expression at the mRNA and protein levels ; however , this phenomenon was not observed in the presence of MMI . - pubmed
Both the cAMP - PKA and PLC - IP3 signaling pathways inhibited the expression of NIS at low iodine concentrations ; however , in thyrocytes treated with high concentrations of iodine , the effect of cAMP - PKA on the expression of NIS changed from inhibition to promotion , while the PLC - IP3 pathway continued to inhibit NIS expression . - pubmed
These findings indicate that intracellular iodinated compounds affect NIS expression through the TSH - mediated cAMP - PKA and PLC - IP3 pathways . - pubmed
Synthesis of fluorescently labelled rhamnosides : probes for the evaluation of rhamnogalacturonan II biosynthetic enzymes . - pubmed
Three fluorescently labelled saccharides 10 - 12 , representing structures found in pectic glycan rhamnogalacturonan II ( RG - II ) , were synthesised by chemical glycosylation of O-6 of diacetone - d - galactose followed by deprotection and reductive amination with amino - substituted fluorophore APTS . - pubmed
APTS - labelled glycans prepared in this manner were purified by carbohydrate gel electrophoresis and subjected to capillary electrophoresis analysis , as a basis for the subsequent development of high sensitivity assays for RG - II - active enzymes . - pubmed
Divergent expression at the RNA and protein level was observed for the metabolic genes pckA and metE , involved in gluconeogenesis and methionine synthesis , respectively . - pubmed
Among them , several compounds displayed promising seizure protection in the maximal electroshock seizure ( MES ) , subcutaneous metrazol seizure ( scMET ) , 6 Hz and pilocarpine - induced status prevention ( PISP ) tests , with ED(50 ) values comparable to the reference anticonvulsant drugs ( AEDs ) . - pubmed
Comparative LC - MS studies on the leaf extract showed isomers of O - caffeoylquinic acid ( 3 - CQA , 4 - CQA and 5 - CQA ) , O - feruloylquinic acid ( 3 - FQA , 4 - FQA and 5 - FQA ) and para - coumaroylquinic acid ( 3 - pCoQA and 5 - pCoQA ) . - pubmed
In this oxidative muscle , Vo ( 2 ) and O ( 2 ) delivery were closely matched during the transition period from rest to steady - state contractions . - pubmed
In conjunction with our previous work showing that speeding O ( 2 ) delivery did not alter Vo ( 2 ) on - kinetics under similar conditions , it appears that spontaneously perfused skeletal muscle operates at the nexus of sufficient and insufficient O ( 2 ) delivery in the transition from rest to contractions . - pubmed
Also , the renal nitrotyrosine level and Txnip mRNA expression were lower when GLN was administered . - pubmed
Examination of the mechanistic activity of MMP9 revealed its capability to degrade the adhesion molecule N - cadherin as well as the basement - membrane protein laminin within or around NCC , respectively . - pubmed
The increased conjugation conferred to the TF dyes by the addition of the pi - conjugated pendant groups increases both their light - harvesting and photovoltaic energy conversion capability in comparison with N749 . - pubmed
This colocalisation is dependent upon an intact microtubule network as depolymerisation by nocodazole treatment abolishes starvation - induced MAP1B recruitment to these vesicles . - pubmed
Both nana and oxNANA display alterations in carbohydrate content , and the extent of these changes varies depending on growth light intensity . - pubmed
Moreover , nana and oxNANA are defective in the expression of some genes implicated in sugar metabolism and photosynthetic light harvesting . - pubmed
These findings suggest that the NANA aspartic protease has an important regulatory function in chloroplasts that not only influences photosynthetic carbon metabolism but also plastid and nuclear gene expression . - pubmed
VESW was associated with an increased incidence of delayed graft function ( DGF ) when steroids were withdrawn within three d post - transplantation . - pubmed
Addiction to cigarette smoking is a prevalent neuropsychiatric disorder that stems from the biophysical and cellular actions of nicotine on nicotinic acetylcholine receptors ( nAChRs ) throughout the central nervous system . - pubmed
Although whole - cell patch clamp recordings from neurons in brain slices is routinely done by the skilled electrophysiologist , it is challenging to locally apply drugs such as acetylcholine or nicotine to the recorded cell within a brain slice . - pubmed
After 2 years , the change in spine BMD was greater in each of the zoledronate groups than in the placebo group ; values are mean ( 95 % confidence interval [ CI ] ) difference versus placebo : zoledronate 1 mg 4.4 % [ 2.7 % to 6.1 % ] ; 2.5 mg 5.5 % [ 3.9 % to 7.2 % ] ; 5 mg 5.3 % [ 3.8 % to 6.7 % ] , p & lt ; 0.001 for each dose ) . - pubmed
Change in BMD at the total hip was greater in each of the zoledronate groups than the placebo group ( mean [ 95 % CI ] difference versus placebo : zoledronate 1 mg 2.6 % [ 1.5 % to 3.7 % ] ; 2.5 mg 4.4 % [ 3.5 % to 5.3 % ] ; 5 mg 4.7 % [ 3.7 % to 5.7 % ] , p & lt ; 0.001 for each dose ) . - pubmed
Each of the bone turnover markers , beta - C - terminal telopeptide of type I collagen ( beta - CTX ) and procollagen type - I N - terminal propeptide ( P1NP ) , was lower in each of the 2.5 - mg and 5 - mg zoledronate groups than the placebo group throughout the trial ( p & lt ; 0.001 versus placebo for each marker for each dose at each time point ) . - pubmed
The potency was predicted for a large set of cyclic amidine and guanidine cores extracted from beta - secretase ( BACE-1 ) inhibitors . - pubmed
The aim of this study was to assess the toxicological risks arising from the coexistence of polyethylene glycol coated single - walled carbon nanotubes ( SWCNTs - PEG ) and a known environmental contaminant : 4 - nonylphenol ( NP ) . - pubmed
Although the results provided no unambiguous evidences of synergistic toxicity between SWCNTs - PEG and NP , some specific treatments with mixtures ( SWCNTs - PEG+NP ) resulted in an unexpected combined toxicity in relation to the individual treatments . - pubmed
Only in those cases the interaction between SWCNTs - PEG and NP could have a synergistic effect on the resulting toxicity . - pubmed
Overall , the serum had a " protective effect " on cells against all treatments : SWCNTs - PEG , NP or their mixtures ( SWCNTs - PEG+NP ) . - pubmed
To determine the normal range of Hemoglobin and cutoff values in healthy adults of Southern India , blood samples were analyzed for parameters of RBC and iron metabolism in 177 male and 203 female medical students . - pubmed
In female students , hemoglobin , hematocrit and parameters of iron metabolism were lower than American white females , except TIBC which was higher . - pubmed
The plasma glucose and insulin levels were analyzed periodically for three hours during the OGTT examinations . - pubmed
Impaired fasting glucose ( IFG ) was defined as a plasma glucose level of & gt;=100 mg / dL to & lt;126 mg / dL. - pubmed
Diabetes was defined as a two - hour post - load plasma glucose level of & gt;=200 mg / dL. - pubmed
BACKGROUND : Monophosphoryl lipid A ( MPLA ) , a nontoxic TLR4 ligand derived from lipopolysaccharide ( LPS ) , is used clinically as an adjuvant in cancer , hepatitis , and malaria vaccines and in allergen - specific immunotherapy . - pubmed
This family - based study analyzed the links between single nucleotide polymorphisms ( SNPs ) in either the POMC or PCSK1 genes and obesity , as well as obesity - related traits among obese Thai children and their families . - pubmed
Participants with the homozygous variant genotype in rs155971 had significantly elevated cholesterol and low - density lipoprotein cholesterol ( LDL - C ) levels ( P = 0.011 , OR = 1.025 , 95%CI = 1.006 - 1.045 ; and P = 0.006 , OR = 1.030 , 95%CI = 1.009 - 1.053 , respectively ) after adjustment for age , gender , and body mass index ( BMI ) . - pubmed
However , variants in PCSK1 did affect cholesterol level , LDL - C level , and waist circumference . - pubmed
Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro - inflammatory response following zinc oxide nanoparticle exposure under different cell culture conditions . - pubmed
In terms of ZnO - NP - induced cytotoxicity pIL8 - RFP cells were the most affected , whilst the pIL8 - Luc were found the least responsive . - pubmed
Indeed , we observe that replication stress induced by the CHK1 inhibitor AZD7762 results in replication catastrophe and apoptosis , when combined with the ATR inhibitor VE-821 specifically in cancer cells . - pubmed
The RD of NDP was 90 mg / m(2 ) during cycles 1 - 3 . - pubmed
Spectroscopic studies performed by NMR , UV - vis and EPR , assisted by theoretical calculations , show that the [ Cu ( no2th )]( 2 + ) complex presents a structure in solution similar to that observed in the solid state . - pubmed
Association of Single Nucleotide Polymorphisms in the Apoptosis - Related Genes TP63 and CD40 with Risk for Lung Cancer in a Chinese Han Population . - pubmed
Polyphenol coatings were applied to chitosan functionalized polypropylene ( PP ) by laccase assisted polymerization of catechol and catechin . - pubmed
Polyphenol coated PP exhibited both metal chelating ( 39.3 + - 2.5 nmol Fe ( 3 + ) cm(-2 ) , pH 4.0 ) and radical scavenging ( up to 52.9 + - 1.8 nmol Trolox eq cm(-2 ) ) capacity , resulting in dual antioxidant functionality to inhibit lipid oxidation and lycopene degradation in emulsions . - pubmed
Nonmigratory polyphenol coated PP inhibited ferric iron promoted degradation better than soluble chelators , potentially by partitioning iron from the emulsion droplet interface . - pubmed
Evoked SPON excitation is comparatively weaker than evoked LSO excitation , likely due to a larger fraction of postsynaptic GluR2 - containing Ca2 + -impermeable AMPA receptors after hearing onset . - pubmed
AREAS COVERED : This review offers an explanation of the pharmacodynamics and of the pharmacokinetics of ospemifene , and gives readers a complete overview of Phase II and III studies on the clinical efficacy , tolerability and safety of this agent in the setting of GSM . - pubmed
Tumor cells do not express an enzyme called arginosuccinate synthetase ( ASS ) and hence , these cells become auxotrophic for arginine . - pubmed
The PARP Inhibitor AZD2461 Provides Insights into the Role of PARP3 Inhibition for Both Synthetic Lethality and Tolerability with Chemotherapy in Preclinical Models . - pubmed
The PARP inhibitor AZD2461 was developed as a next - generation agent following olaparib , the first PARP inhibitor approved for cancer therapy . - pubmed
Here we demonstrate the efficacy of this compound against olaparib - resistant tumors that overexpress P - glycoprotein . - pubmed
Alkali and alkaline earth metal salts with lithium ( 1 ) , sodium ( 2 ) , potassium ( 3 ) , rubidium ( 4 ) caesium ( 5 ) , magnesium ( 6 ) , calcium ( 7 ) , strontium ( 8) and barium ( 9 ) were prepared and fully characterized using elemental analysis , IR and NMR spectroscopy , DSC and TG analysis . - pubmed
The four groups used in study were : 2 % chlorhexidine digluconate , 2 % doxycycline solution , 5 % Proanthocyanidin ( PR ) , Control Group . - pubmed
Amongst all the etching pretreatment groups , CHE group ( Chlorhexidine etching group ) revealed highest exposure to collagen fibrils than the other groups of etching . - pubmed
CONCLUSION : The PDE4 inhibitor roflumilast has a favorable side - effect profile at a cognitively effective dose and could be considered as a treatment in disorders affected by disrupted sensory gating . - pubmed
Involvement of mitogen activated kinase kinase 7 intracellular signalling pathway in Sunitinib - induced cardiotoxicity . - pubmed
The tyrosine kinase inhibitor Sunitinib is used to treat cancer and is linked to severe adverse cardiovascular events . - pubmed
Apoptosis signal - regulating kinase 1 ( ASK1 ) is the upstream activator of MKK7 and is specifically inhibited by 2,7 - dihydro-2,7 - dioxo-3H - naphtho [ 1,2,3 - de ] quinoline-1 - carboxylic acid ethyl ester ( NQDI-1 ) . - pubmed
This study investigates the role of ASK1 , MKK7 and JNK during Sunitinib - induced cardiotoxicity . - pubmed
Administration of Sunitinib ( 1 muM ) during Langendorff perfusion resulted in increased infarct size , increased miR-133a expression , and decreased phosphorylation of the ASK1 / MKK7 / JNK pathway compared to control . - pubmed
Co - administration of NQDI-1 ( 2.5 muM ) attenuated the increased Sunitinib - induced infarct size , reversed miR-133a expression and restored phosphorylated levels of ASK1 / MKK7 / JNK . - pubmed
These findings suggest that the ASK1 / MKK7 / JNK intracellular signalling pathway is important in Sunitinib - induced cardiotoxicity . - pubmed
Investigations into the ASK1 / MKK7 / JNK transduction pathway could lead to development of cardioprotective adjunct therapy , which could prevent Sunitinib - induced cardiac injury . - pubmed
Aerobic granular sludge was cultivated in 44 days ( R1 ) and 25 days ( R2 ) under the conditions of high settling velocity ( 18 m h-1 ) and high organic loading rate ( OLR ) ( 2.1 - 2.4 kg COD m3 day ) . - pubmed
The Deubiquitinase OTU5 Regulates Root Responses to Phosphate Starvation . - pubmed
This divide and conquer strategy is first demonstrated in the fitting of PES for OH3 with Gaussian process regression ( GPR ) and further applied to construct PESs for CH5 and O+CH4 with artificial neural networks ( NN ) . - pubmed
Methods : Using experimental DBS from the blood of an adult volunteer , we optimized extraction of small molecules and integrated measurement of potassium as a proxy for blood hematocrit . - pubmed
Normalizing for potassium levels removed variability related to varying hematocrit across DBS punches . - pubmed
Aldosterone is synthesized in zona glomerulosa of adrenal cortex in response to angiotensin II . - pubmed
The physiological significance of Nurr1 in aldosterone production in adrenal cortex has been well studied . - pubmed
Furthermore , PARP1 inhibitor also suppressed the aldosterone secretion in response to the angiotensin II . - pubmed
In addition , iodocyclization of fluorinated propargylamine affords chiral 3 - iodo-2 -( trifluoromethyl )- 1,2 - dihydroquinoline , which can be easily converted to 2 -( trifluoromethyl )- 1,2 - dihydroquinoline derivatives with the selective COX-2 inhibitory activity . - pubmed
Simultaneous incubation with verapamil showed that P - glycoprotein does not play a relevant role on CA and KA oral absorption , with Papp of 4.48 ( + -0.26 ) x 10 - 6 cm / s and 5.37 ( + -0.72 ) x 10 - 6 cm / s observed for CA and KA , respectively . - pubmed
B-0831 ( Ps3alphaHSD ) , which forms a dimer composed of two 26 kDa subunits , we expressed and purified 15N - Tyr labeled Ps3alphaHSD and its mutants , and analyzed the conformational change upon NADH binding . - pubmed
They were found to have low serum phosphorus , normal calcium , and vitamin D studies , with elevated alkaline phosphatase suggestive of nutritional hypophosphatemia . - pubmed
VraS is a histidine kinase that undergoes autophosphorylation in the presence of signals elicited upon cell wall damage and it then transfers its phosphoryl group to VraR. - pubmed
A single point mutation in the dimerization interface of VraR , in which Met13 was replaced by Ala , led to the inability of VraR to dimerize and to bind optimally to the target promoter . - pubmed
The BTD transition has also been suggested to be valid for oxide glasses , but , unfortunately , direct prediction of Poisson 's ratio from chemical composition remains challenging . - pubmed
TTP399 is a hepatoselective , glucokinase activator with potential for treating type 2 diabetes . - pubmed
In situ heating TEM observations of evolving nanoscale Al - Mg - Si - Cu precipitates . - pubmed
This research concerns the precipitation and subsequent dissolution of precipitate phases in an Al-0.86Mg-0.62Si-0.1Cu ( at.% ) alloy subjected to varying thermal exposure during an in situ heating TEM experiment . - pubmed
CONCLUSION : The standard treatment of hormone receptor positive and HER-2 negative metastatic breast cancer is everolimus together with exemestane . - pubmed
In this scenario , inosine is a purine nucleoside known to act as an agonist of A1 and A2A adenosine receptors . - pubmed
Inosine pretreatment ( 25 mg / kg , oral route ) prevented the hyperlocomotion induced by ketamine ( 25 mg / kg , intraperitoneal route ) in the open - field test ; however , there was no difference in hippocampal density of A1 and A2A receptors , where ketamine , as well as inosine , were not able to promote changes in immunocontent of the adenosine receptors . - pubmed
In this sense , we suggest further investigation of signaling pathways involving purinergic receptors , using pharmacological strategies to better elucidate the action mechanisms of inosine on bipolar disorder . - pubmed
Specific cleavage of the recombinant proenzyme leads to the activation of proPAO , followed by oxidation and oxygenation of l - phenylalanine , resulting in an increase of chromogenic production when coupled with the Trinder reaction . - pubmed
RESULTS : We applied this method to determine the activities of both coagulation factor IIa and plasmin in their physiologically relevant basal state and fully activated state in sodium citrate - anticoagulated plasma respectively . - pubmed
We aim to differentiate the effects of acute beta - adrenergic stimulation ( beta - ARS ) and cardiomyocyte stretch ( increased sarcomere length ) on calcium - transient dynamics and force generation , using a novel computational model of cardiac electromechanics . - pubmed
An increase in beta - ARS increased the amplitudes of force ( augmented inotropy ) and calcium transient , and shortened both force and calcium - transient duration ( lusitropy ) . - pubmed
The paracaspase mucosa - associated lymphoid tissue lymphoma translocation protein-1 ( MALT1 ) regulates nuclear - factor - kappa - B ( NF - kappaB ) activation downstream of surface receptors with immunoreceptor tyrosine - based activation motifs ( ITAMs ) , such as the B - cell or T - cell receptor and has thus emerged as a therapeutic target for autoimmune diseases . - pubmed
Daily administration of the placebo solution , resveratrol ( 10 mg / kg ) , and ZLD ( 0.1 mg / kg ) was carried out for a period 42 days prior to initiation of EP , and then for another 28 days following ligature placement . - pubmed
IL-4 levels were significantly lower in the OVT + PLAC group versus OVT + ZLD + RESV and SHAM + PLAC ( P < .05 ) . - pubmed
NADPH oxidase ( nicotinamide adenine dinucleotide phosphate oxidase ) levels were significantly lower OVT + RESV group when compared to OVT + PLAC ( P < .05 ) . - pubmed
RESULTS : The addition of DMOG resulted in HIF-1alpha stabilization in the exterior of the engineered constructs , which resulted in increased regional type II collagen deposition , but the stabilization did not translate to overall increased extracellular matrix deposition . - pubmed
CONCLUSIONS : Despite previous studies that demonstrated the efficacy of DMOG , here , short - term treatment with DMOG did not have a uniformly positive impact on the chondrogenic capacity of SYN - MSCs in either pellet culture or in scaffold - based engineered cartilage , as evidenced by reduced matrix production . - pubmed
Galeterone sensitizes breast cancer to chemotherapy via targeting MNK / eIF4E and beta - catenin . - pubmed
In our work , galeterone as a chemical compound under clinical trials for the treatment of prostate cancer , was identified to be effective in targeting breast cancer cells via suppressing MNK - eIF4E and beta - catenin . - pubmed
Mechanism analysis demonstrated that galeterone decreased MNK1 / 2 level and phosphorylation of eIF4E. - pubmed
In addition , galeterone decreased beta - catenin level via promoting GSK-3beta - mediated beta - catenin degradation , and furthermore that Akt but not CK1 was involved in beta - catenin degradation by galeterone . - pubmed
Rescue studies demonstrated that both MNK / eIF4E and beta - catenin were responsible for anti - breast cancer activity of galeterone . - pubmed
Loss and gain of function of PDCD4 - AS1 , miR-10b-5p or IQGAP2 were performed before MTT and colony formation assay , TUNEL staining in addition to Transwell and scratch assays were applied to measure the cell biological functions . - pubmed
Stereospecific Response of E / Z - isomers of N - Nitrososarcosine in LC - ESI - MS / MS . - pubmed
Biochemical analyses of isolated Muller cells showed that ( a ) these cells did not synthesize and accumulate acetylcholine , gamma - aminobutyric acid , or catecholamines when incubated with appropriate radioactive precursors ; ( b ) the specific activities of choline acetyltransferase ( EC 2.3.1.6 ) , glutamate decarboxylase ( EC 4.1.1.15 ) , and tyrosine hydroxylase ( EC 1.14.16.2 ) in these cells were less than 2 percent of those found in the retina ; ( c ) Muller cells , however , contained high activities of transmitter degrading enzymes - acetylcholinesterase ( EC 3.1.1.7 ) and gamma - aminobutyrate - transamine ( EC 2.6.1.19 ) ; and ( d ) the cells also possessed high levels of two presumably glial - specific - enzymes - glutamine synthetase ( EC 6.3.1.2 ) and carbonic anhydrase ( EC 4.2.1.1 ) . - pubmed
The findings suggest that alteration of the cell surface membrane and microfilaments prevents damage by interfering with cell - target interactions , that energy derived from glycolysis is required for cytotoxicity , that cell - associated esterases are probably involved , and that cytotoxicity may be modulated by cyclic nucleotides . - pubmed
As more has become known about the mechanisms by which abnormal levels of circulating lipoproteins promote atherosclerosis , certain risk factors have emerged as concerns for women , including : ( 1 ) diabetes mellitus as a risk factor , perhaps through its more profound effects on circulating lipoproteins ; ( 2 ) serum triglyceride levels , and ( 3 ) changes in high - density lipoprotein cholesterol . - pubmed
This suggests that the high - affinity catalytic site presents a hydrophobic environment for ATP binding in native enzyme , that there is a significant difference between the conformation for ADP binding as opposed to ATP binding , and that the ADP - binding conformation is more hydrophilic . - pubmed
Cytosolic diacylglycerol kinase was irreversibly inactivated by 5 '- AMP since the enzyme remained less active after the removal of 5 '- AMP by P-10 gel chromatography . - pubmed
The value of penicillinase sensitive penicillins in treating superficial wound infections is questioned . - pubmed
The ATPase inhibitor dicyclohexylcarbodiimide ( DCCD ) specifically inhibited sulfate reduction but had no immediate influence on sulfate accumulation . - pubmed
The K +- H +- antiporter nigericin ( in 150 mM KCl ) and the Na +- H +- antiporter monensin ( in 150 mM NaCl ) caused partial inhibition of sulfate accumulation , whereas the K+-transporter valinomycin ( in 150 mM KCl ) and the Na+-H+ exchange inhibitor amiloride ( 2 mM ) were without effect. - pubmed
In contrast , postischemia treatment with pentoxifylline was associated with significant recovery in CIN and UFR , and stable RVR , RBF , and RBP . - pubmed
These findings offer further evidence for a primary role of prolactin in lipid metabolism and indicate that bromocriptine may be useful for treating obesity and lipid - based cardiovascular disorders . - pubmed
Effects of prenatal exposure to ethanol on neocortical development : II . - pubmed
Fetuses were administered with [ 3H ] thymidine on G13 , G17 , or G20 and killed 45 - 60 minutes later . - pubmed
TBPA was isolated by retinol - binding protein ( RBP ) affinity chromatography and further purified by preparative agarose gel electrophoresis or FPLC ion exchange chromatography . - pubmed
TBG was purified by thyroxine ( T4)-Sepharose chromatography followed by gel filtration on Sephacryl S-300 and preparative electrofocusing in a granulated dextran gel . - pubmed
Gel filtration of serum on Sephacryl S-200 , identification of T4 - binding proteins with 125I - T4 , and protein- and lipoprotein staining of fractions was performed . - pubmed
Thyroxine - binding was found to TBG in the beta - globulin region , TBPA in the alpha 2 - region , albumin , and to the high density lipoprotein ( HDL2 ) in the alpha 1 - region and the very low density lipoprotein ( VLDL ) in the pre - beta region . - pubmed
A corresponding band to the latter protein in serum was masked by TBG and TBPA , and T4 - binding in the alpha 1 - region was not always seen in serum . - pubmed
It turned out that , if the first postirradiation cell division occurs at higher KCl concentrations ( " rapid " postirradiation recovery is inhibited ) , the frequency of mitotic reciprocal recombination within the gene ADE2 - centromere region decreases . - pubmed
Keeping of irradiated cells ( in the G1 phase of the cell division cycle ) in water at 28 degrees C prior to plating on the selective agar containing 1.5 M KCl leads to smaller frequency of gene conversion lys2 - 25 / lys2 - 22 - ---Lys+ , as compared with that for the cells immediately plated on the selective agar . - pubmed
Methoctramine reveals heterogeneity of M2 muscarinic receptors in longitudinal ileal smooth muscle membranes . - pubmed
Methoctramine , an atrial selective muscarinic receptor antagonist in functional studies was , however , able to partially discriminate between these two receptors in our binding studies . - pubmed
The photomyoclonic reflex ( PMR ) , consisting of a one- or two - component blink reflex associated with the flash electroretinogram ( ERG ) , is sensitive to the acute effect of intravenously administered diazepam . - pubmed
Chromogranin represents a family of proteins that coexist with catecholamines in the secretory vesicles of adrenal medulla cells . - pubmed
Basal plasma GH concentrations before hGHRH-44 administration were not statistically different in the two experiments [ glucose experiment , 2.1 + /- 0.1 ( + /- SE ) ng / ml ; water experiment , 2.6 + /- 0.6 ng / ml ] . - pubmed
An examination of the zinc requirement for the catalytic activity of 3 - phosphoglyceraldehyde dehydrogenase . - pubmed
Detection of epsilon ( gamma - glutamyl ) lysine crosslinks is not only necessary for establishing the importance of the dipeptide as a post - translational modification of proteins , but provides information as to the importance of the transglutaminase enzyme in a biological system . - pubmed
Quantitative estimation of epsilon ( gamma - glutamyl ) lysine normally involves its further separation by ion - exchange chromatography and its post - column detection following derivatisation with ninhydrin . - pubmed
If there is only moderate mucosal damage , a rather weak correlation is seen either for the trehalose - load - test or for the disaccharidase activities with morphological changes . - pubmed
Both methods in comparison show a better correlation between trehalase activity and morphology than between trehalose - load - test and morphology . - pubmed
Upon recirculation of the brain , phosphocreatine , ATP , and lactate concentrations recovered to control values in the paramedian neocortex and CA1 zone of hippocampus and to near - control values in the striatum . - pubmed
The phosphocreatine and ATP concentrations then fell and the lactate levels rose in the striatum after 6 - 24 h , and in the CA1 zone of hippocampus after 24 - 72 h. The initial recovery and subsequent delayed changes in the phosphocreatine , ATP , and lactate concentrations in the striatum and hippocampus coincided with the onset and progression of morphological injury in these brain regions . - pubmed
It was shown to fractionate into at least 11 components that can be detected by coomassie blue staining , a radioligand - receptor assay ( RRA ) , and four hCG RIAs using antisera against the purified hCG , its alpha- and beta - subunits , and the hCG beta carboxyl - terminal peptide . - pubmed
Thus , sialic acid alone and/or differences in subunit assembly seem to be responsible for the electrophoretic heterogeneity of highly purified hCG . - pubmed
We show that the translocation t(12;16)(q13 : p11 ) in malignant myxoid liposarcoma can be a fusion of the CHOP dominant negative transcription factor gene with a novel gene , FUS , which can result in fusion of the FUS glycine - rich protein with the whole CHOP coding region . - pubmed
The effect of progesterone on sulfate uptake was abolished by blocking either the cAMP pathway or the genomic action of progesterone and was independent of the cAMP - activatable apical chloride channel . - pubmed
To clarify the role of serotonin , the in vivo and in vitro effects of the selective 5 - HT2 receptor antagonist , DV-7028 , on MCT - induced pulmonary hypertension were studied . - pubmed
After two months the mean blood sugar level rose again insignificantly and an insignificant decline of glycosylated haemoglobin occurred . - pubmed
Nucleotide binding to the hydrophilic C - terminal domain of the transporter associated with antigen processing ( TAP ) . - pubmed
NBD - TAP1 bound to C-8 - ATP - agarose and was specifically eluted with ATP or EDTA . - pubmed
Photoaffinity labeling of NBD - TAP1 with the ATP analogues 8 - azido -[ gamma-32P ] ATP and 3 '- O -[( 4 - azido-3,5 -[ 125I ] diiodo-2 - hydroxybenzoyl )- beta - alanyl ]- ATP was specific . - pubmed
ATP hydrolysis by NBD - TAP1 was not detected . - pubmed
The described experiments demonstrate ATP binding to an isolated domain of the antigenic peptide transporter , TAP , and therefore support the theory of ATP - dependent peptide translocation . - pubmed
One was a rapidly activating and slowly inactivating type of outward rectifier ( named IK ( FR ) for fast rectifier potassium current ) , similar to a potassium current described in cardiac muscle , and the other was a transient A - current ( IA ) . - pubmed
The thermal stability of ceruloplasmin , as studied by differential scanning calorimetry , was affected by calcium but not by magnesium . - pubmed
The interaction of ceruloplasmin with the chromatographic material used for its isolation , Sepharose 4B derivatized with chloroethylamine , was weakened by calcium . - pubmed
Isolation of a recombinant intracellular beta - galactosidase by ammonium sulfate fractionation of cell homogenates . - pubmed
As the ammonium sulfate concentration increased , the enzyme was precipitated preferentially and at 30 % salt saturation nearly all had been recovered in the precipitate . - pubmed
A significant increase in plasma cord blood cotinine was detected in Group II , newborns whose mothers were active cigarette smokers throughout the pregnancy . - pubmed
A genetic algorithm for designing gene family - specific oligonucleotide sets used for hybridization : the G protein - coupled receptor protein superfamily . - pubmed
A set of 15 oligos between 7 and 14 bases in length has correctly identified 70 % of the GPCR cDNA collection sequences with 0.02 % false positives . - pubmed
The tumor LR expression was immunohistochemically determined in paraffin - embedded sections using the MLuC5 monoclonal antibody which recognizes the 67 - kDa LR and the avidin - biotin immunoperoxidase method . - pubmed
Oxidative bioactivation of the lactol prodrug of a lactone cyclooxygenase-2 inhibitor . - pubmed
The lactol derivative of a lactone cyclooxygenase-2 inhibitor ( DFU ) was evaluated in vivo and in vitro for its potential suitability as a prodrug . - pubmed
TSP2 - null fibroblasts grew as aggregates on both surfaces , and their attachment was further compromised on ox - PDMS . - pubmed
METHODS : Recombinant adenoviruses expressing a series of C - CAM1 mutants were generated , such as AdCAMF488 ( mutated C - CAM1 containing Tyr-488 --> ; Phe-488 ) , AdCAMH458 ( intracellular domain deletion mutant containing 458 amino acids ) , AdCAMG454 ( intracellular domain deletion mutant containing 454 amino acids ) , and AdCAMDeltaD1(C - CAM1 mutant containing first Ig domain deletion ) . - pubmed
The EP4 receptor antagonist AH23848B ( [ 1alpha ( z ) , 2beta5alpha ]-(+/-)- 7 -[ 5 -[[( 1,1 '- biphenyl )- 4 - yl ] methoxy ]- 2 -( 4 - morph olinyl )- 3 - oxo - cyclopentyl ]- 4 - heptenoic acid ) ( 29 microM ) had no effect on concentration - effect curves to the EP receptor agonists . - pubmed
The mixed prostanoid receptor antagonist AH6809 ( 6 - isopropoxy-9 - oxaxanthene-2 - carboxylic acid ) competitively antagonised prostaglandin E2 with a pA2 of 5.6+/-0.2 . - pubmed
AH6809 ( 42 microM ) antagonised misoprostol , 11 - deoxy prostaglandin E1 , and the prostanoid DP receptor agonist BW245C ( 5 -( 6 - carboxyhexyl )- 1 -( 3 - cyclohexyl-3 - hydroxypropyl ) hydantoin ) with apparent pA2 values of 5.6+/-0.3 , 5.1+/-0.9 and 5.9+/-0.4 ( n = 4 ) , respectively , but was ineffective against the IP receptor agonist cicaprost ( n = 4 ) . - pubmed
The prostanoid DP receptor antagonist BW A868C ( 3 - benzyl-5 -( 6 - carboxyhexyl )- 1 -( 2 - cyclohexyl-2 - hydroxyethylamino ) h ydantoin ) ( 50 nM ) had no effect on responses to prostaglandin E2 or misoprostol . - pubmed
The resulting ubiquitin - protein conjugates are degraded by the 26S proteasome , an ATP - dependent protease . - pubmed
Dimerization of sterol regulatory element - binding protein 2 via the helix - loop - helix - leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta . - pubmed
The sterol regulatory element - binding protein 2 ( SREBP-2 ) , a transcription factor of the basic helix - loop - helix - leucine zipper ( bHLH - Zip ) family , is synthesized in the form of a membrane - attached precursor molecule . - pubmed
When cells are deprived of sterols , a two - step proteolytic processing releases the transcriptionally active N - terminal segment of SREBP-2 , thereby allowing it to enter the nucleus . - pubmed
It was found that the active form of SREBP-2 exists as a stable dimer in solution and that the substitution of leucine residues for alanine in the leucine zipper motif disrupted the dimerization . - pubmed
Activation of p53 transcriptional activity requires ATM 's kinase domain and multiple N - terminal serine residues of p53 . - pubmed
Finally , single point - mutations of serines 15 , 20 , 33 or 37 did not individually block the ATM - dependent activation of p53 transcriptional activity by bleomycin . - pubmed
However , double mutations of either serines 15 and 20 or serines 33 and 37 blocked the ability of ATM to activate p53 . - pubmed
In addition , activation of p53 by ATM requires multiple serine residues in p53 's transactivation domain . - pubmed
A survey was sent to programs to assess the processes in place to assist patients in managing cholesterol and reaching a low - density lipoprotein ( LDL - C ) goal of less than 100 mg / dL. - pubmed
The Y238 DAAO mutants have spectral properties similar to those of the wild - type enzyme but the degree of stabilization of the flavin semiquinone and the redox properties in the free form of Y238S are different . - pubmed
The purpose of Part II was to further establish concurrent validity of the PROBS-14 with known pediatric behavior measures ( TRF , CTRS ) and to ascertain if the PROBS-14 better predicts a cocaine behavioral effect . - pubmed
The PROBS instrument offers a promising brief measure of child problem behaviors with adequate concurrent and predictive validity , and which outperforms the TRF and CTRS in discriminating prenatal cocaine exposure status . - pubmed
Selective serotonin reuptake inhibitors were most commonly prescribed , followed by tricyclic antidepressants , atypical and third - generation antidepressants , and monoamine oxidase inhibitors . - pubmed
Using whole - cell patch clamp experiments , a novel chloride current was identified . - pubmed
The inwardly rectifying rClC-2 chloride current was activated by hyperpolarization but not by pH variation . - pubmed
A different depolarization - activated outwardly rectifying chloride current was activated only by hypotonic challenge and may correspond either to rClC-3 or rClC-6 . - pubmed
Based on our molecular analysis of rClC proteins , this new chloride current does not correspond to rClC-2 , rClC-3 , rClC-6 , or rClC-7 channels . - pubmed
Exposure to high concentrations of glucose and insulin results in insulin resistance of metabolic target tissues , a characteristic feature of type 2 diabetes . - pubmed
High glucose ( HG ) induced a significant decrease in insulin - stimulated glucose uptake [ tracer - determined disappearance rate ( Rd ) , control 41.2 + /- 1.7 vs. HG 32.4 + /- 1.9 mg . - pubmed
The effect of hyperventilation and probanthine on isolated RS - T segment and T - wave abnormalities . - pubmed
[ Blood transaminases as indication of tolerance to antidiabetic sulfanylurea ] . - pubmed
CONCLUSION : Amongst the differentially - expressed genes correlating with imatinib resistance , several suggest the activation of alternative pathway(s ) that maintain viability and growth independently of Bcr - Abl kinase activity . - pubmed
The discovery and synthesis of novel adenosine substituted 2,3 - dihydro-1H - isoindol-1 - ones : potent inhibitors of poly ( ADP - ribose ) polymerase-1 ( PARP-1 ) . - pubmed
We found that potent inhibition of poly ( ADP - ribose ) polymerase-1 ( PARP-1 ) was achieved when isoindolinone was linked to adenosine by a spacer group of a specific length . - pubmed
The removal of raffinose was due to the high alpha - galactosidase ( alpha - gal ) activities of these lactic acid bacteria , which was highest at pH 5.5 ( 5.0 U / ml ) . - pubmed
[ Effect of caffeine on amylases ] . - pubmed
Control of cytochrome c oxidase activity by nitric oxide . - pubmed
Over the past decade it was discovered that , over - and - above multiple regulatory functions , nitric oxide ( NO ) is responsible for the modulation of cell respiration by inhibiting cytochrome c oxidase ( CcOX ) . - pubmed
Silibinin down - regulated PSA mRNA expression and PSA secretion in conditioned medium under basal and DHT ( 10(-8 ) M ) stimulated conditions , which was paralleled by PDEF down - regulation . - pubmed
DHT alone stimulated PDEF and PSA gene expression and PSA secretion . - pubmed
The down - regulation of basal as well as DHT stimulated PDEF and PSA by silibinin demonstrates the antiproliferative potential of this agent . - pubmed
We show that doses of gamma - radiation ( IR ) as low as 0.25 Gy , which generate vast numbers of SSBs but only a few DSBs per cell ( & lt;8 ) , promptly activate ATM kinase and induce the phosphorylation of the ATM substrates p53 - Ser15 , Nbs1 - Ser343 and Chk2 - Thr68 . - pubmed
1 Gy ) , which cause Chk2 autophosphorylation on Thr387 , Chk2 - dependent accumulation of p21waf1 and checkpoint arrest in the S phase . - pubmed
Compared with the nonfluorinated parent compounds 1i-1 , fluorinated 1 -( naphth-2 - yl ) methylimidazoles 1a - h turned out to be potent inhibitors of CYP17 and CYP19 enzymes . - pubmed
In order to elaborate the function of different types of opioid receptors in regulating melatonin synthesis , we used a selective mu - opioid receptor agonist , Tyr -[ D - Ala(2 ) , N - methyl - phe(4 ) , glycol(5 ) ] ( DAMGO ) , a selective delta - opioid receptor agonist , Enkephalin [ D - Pen(2 ) , D - Pen(5 ) ] , ( DPDPE ) and a selective kappa - opioid receptor agonist , ( ( + ) -( 5alpha , 7alpha , 8beta )- N - methyl - N -[ 7 - ( 1 - pyrrolidinyl )- 1 - oxaspiro [ 4,5 ] dec-8 - yl ]- benzene acetamide ) ( U69593 ) to investigate the activity of N - acetyltransferase ( NAT ) activity and melatonin secretion . - pubmed
These stimulatory effects were blocked by naloxone , an opioid receptor antagonist . - pubmed
However , the kappa - opioid receptor agonist U69593 was unable to alter either the activity of NAT or the level of melatonin . - pubmed
This study reveals that the stimulatory effect of opioid receptor on melatonin synthesis is mediated via the activation of adenylate cyclase system . - pubmed
He was given the gonadotropin - releasing hormone agonist leuprolide acetate and the androgen receptor antagonist flutamide for the treatment of prostate cancer . - pubmed
After the second injection of leuprolide acetate , fasting glucose and hemoglobin A1c ( HbA1c ) levels were found to be markedly elevated ( 22.8 mmol / L and 10.5 % , respectively ) . - pubmed
After improvement of hyperglycemia by insulin treatment , their glycemic control remained good after treatment with pioglitazone without use of insulin . - pubmed
Natural rhodopsin contains an 11 - cis - retinylidene chromophore . - pubmed
Adenosine deaminases that act on RNA ( ADARs ) catalyze the site - specific conversion of adenosine to inosine in primary mRNA transcripts , thereby affecting coding potential of mature mRNAs . - pubmed
Structural determinants that define the adenosine moieties for specific ADARs - mediated deaminations are currently unknown . - pubmed
Erlotinib ( Tarceva , OSI-774 ; Pfizer , Inc. ) is an orally - active , targeted inhibitor of the epidermal growth factor receptor ( EGFR / HER1 ) , which is part of a key regulatory pathway in cancer . - pubmed
They were placed on a 6 - week cycle regimen comprising capecitabine ( X ; 2,000 mg / m2 / day in two divided doses for 2 weeks then 1 week rest ) , idarubicin ( I ; 10 mg / m2 / day , days 1 , 3 and 5 ) and cyclophosphamide ( C ; 100 mg / m2 / day for 2 weeks then 1 week rest ) . - pubmed
Molecular aspects of gefitinib antiproliferative and pro - apoptotic effects in PTEN - positive and PTEN - negative prostate cancer cell lines . - pubmed
We reported that the EGFR - selective tyrosine kinase inhibitor gefitinib ( ZD1839 ; Iressa ) is able to induce growth inhibition , G(1 ) arrest and apoptosis in PCa cells and that its effectiveness is associated primarily with phosphatase and tensin homologue deleted from chromosome 10 ( PTEN ) expression ( and thus Akt activity ) . - pubmed
In fact PTEN - negative PCa cells are slowly sensitive to gefitinib treatment , because this molecule is unable to downregulate PI3K / Akt activity . - pubmed
PI3 K inhibition , by LY294002 or after PTEN transfection , restores EGFR - stimulated Akt signalling and sensitizes the cells to pro - apoptotic action of gefitinib . - pubmed
Thus the effectiveness of gefitinib requires growth factor receptor - stimulated PI3K / Akt and MAPK signalling to be intact and functional . - pubmed
The loss of the PTEN activity leads to uncoupling of this signalling pathway , determining a partial gefitinib resistance . - pubmed
Moreover , gefitinib sensitivity may be maintained in these cells through its inhibitory potential in MAPK / ERK pathway activity , modulating proliferative EGFR - triggered events . - pubmed
Moreover , E-64 , an inhibitor of CTSK , could prevent adipocyte differentiation in a dose - dependent manner , which was characterized by the absence of triglyceride accumulation and glycerol contents . - pubmed
CONCLUSIONS : CTSK , a cysteine protease involved in extracellular matrix remodeling , could be one of the determinants of adipocyte differentiation . - pubmed
Ascorbate itself is oxidized to dehydroascorbate during the process of antioxidation , and dehydroascorbate reductase ( DHAR , EC 1.8.5.1 ) re - reduces the oxidized ascorbate . - pubmed
Salt tolerance was remarkably improved despite slight increases in DHAR activity and total ascorbate . - pubmed
By catabolizing tryptophan to N - formyl - kynurenine , IDO starves T cells from this important amino acid rendering them incapable of mounting appropriate immune responses . - pubmed
IDO - induced tryptophan depletion from the tumor microenvironment could be the result of either elevated levels of the enzyme or augmented tryptophan consumption by both tumor cells and antigen presenting cells of the host . - pubmed
Selenium inhibits high glucose- and high insulin - induced adhesion molecule expression in vascular endothelial cells . - pubmed
Selenium ( Se ) , a biologically essential trace element , can inhibit cytokine ( e.g. - pubmed
These experiments tested whether Se can also alter high glucose- and high insulin - induced expression of adhesion molecules . - pubmed
RESULTS : Se ( 100 nmol / L ) significantly inhibited glucose ( 25 mmol / L)-induced expression of vascular cell adhesion molecule-1 ( VCAM-1 ) , intercellular adhesion molecule-1 ( ICAM-1 ) , and E - selectin . - pubmed
Moreover , Se significantly inhibited insulin ( 100 nmol / L)-induced VCAM-1 and ICAM-1 expression , whereas high insulin had no inducing effect on E - selectin . - pubmed
Se also inhibited high glucose- and high insulin - induced activation of p38 mitogen - activated protein kinase ( p38 ) , which indicated that the preventive effects of Se on adhesion molecules may be associated with p38 . - pubmed
The important role of p38 in Se effects was further confirmed using p38 inhibitor SB203580 . - pubmed
CONCLUSIONS : These results suggest that Se can inhibit high glucose- and high insulin - induced expression of adhesion molecules . - pubmed
We also show that premature delivery is controlled by treatment with the selective endothelin receptor A antagonist BQ-123 in a dose - dependent manner . - pubmed
Gender - associated biological characteristics , likely independent of circulating gonadal steroids , thus seem to influence HPA - axis regulation in depression . - pubmed
Infusion media composition is phosphate - buffered saline containing anticoagulants and human serum albumin . - pubmed
The time course of hepatocyte viability , funtionality ( drug - metabolizing enzymes , ureogenic capability , ATP , glycogen , and GSH levels ) , apoptosis ( caspase-3 activation ) , and attachment and monolayer formation were analyzed . - pubmed
The optimal preservation of cell viability , attaching capacity , and functionality , particularly GSH and glycogen levels , as well as drug - metabolizing cytochrome P450 enzymes , was found in infusion media supplemented with 2 mM N - acetyl - cystein and 15 mM glucose . - pubmed
The results presented here represent the first report of a kaempferol glycoside as a putative efflux pump inhibitor in bacteria . - pubmed
However , the dolicholpyrophosphate - binding antibiotic bacitracin failed to inhibit the acid PPase . - pubmed
The purified enzyme showed similar K(m ) values for PPi , ATP , and ADP to the membrane - bound enzyme . - pubmed
Serum vitamin D , calcium , phosphate , and parathyroid hormone ( PTH ) concentrations were measured . - pubmed
However , we observed a significant inverse correlation between vitamin D concentrations and PTH . - pubmed
Lower vitamin D levels are associated with a higher PTH concentration . - pubmed
Down - regulation of Nrf2 enhanced the cytotoxicity of Oxaliplatin , Doxorubicin and Etopside . - pubmed
Down - regulation of Nrf2 enhanced the cytotoxicity of Oxaliplatin , Doxorubicin and Etopside . - pubmed
The deduced amino acid sequence of SoISG15 shares 60 - 67 % overall identities with the ISG15 of several fish species . - pubmed
In this study , we investigated the effects of zeaxanthin on the migration of skin fibroblasts induced by PDGF - BB and melanoma cells . - pubmed
We demonstrated that zeaxanthin inhibited PDGF - BB - induced skin fibroblast migration on collagen and gelatin by a modified Boyden chamber system . - pubmed
However , these effects did not result from direct interaction of zeaxanthin with PDGF - BB . - pubmed
Thus , our results provide the first evidence showing that zeaxanthin is an effective inhibitor of migration of stromal fibroblasts induced by PDGF - BB and melanoma cells and this effect may further support its antitumor potential . - pubmed
Strong catabolic repression on protease and alpha - amylase production was observed with glucose . - pubmed
Exercise training reduced epididymal fat , fasting serum insulin and plasma glucose disappearance . - pubmed
We describe the development of the Patient Satisfaction with Treatment Benefit ( PSTB ) questionnaire , and examine the face , content and criterion validity of this tool in a study of darifenacin treatment in OAB patients who expressed dissatisfaction with prior antimuscarinic therapy . - pubmed
After the LH peak , the intrafollicular concentration of 17beta - estradiol decreased significantly ( 48.3 + /- 4.4 ng / ml ; p & lt ; 0.001 ) , whereas that of progesterone increased ( 11690.2 + /- 693.6 ng / ml ; p & lt ; 0.001 ) . - pubmed
Invariant natural killer T ( iNKT ) cells produce large amounts of cytokines such as IL-4 and IFNgamma upon stimulation with a ligand , alpha - galactosylceramide , and regulate various immune responses . - pubmed
Vanilloid derivatives as tyrosinase inhibitors driven by virtual screening - based QSAR models . - pubmed
A number of vanilloids have been tested as tyrosinase inhibitors using Ligand - Based Virtual Screening ( LBVS ) driven by QSAR ( Quantitative Structure - Activity Relationship ) models as the multi - agent classification system . - pubmed
Among them , two chemicals , isovanillin ( K(M ) ( app ) = 1.08 mM ) near to kojic acid ( reference drug ) in one cluster and isovanillyl alcohol ( K(M ) ( app ) = 0.88 mM ) at the same distance as hydroquinone ( reference drug ) in another cluster showed inhibitory activity against tyrosinase . - pubmed
Glutamate cysteine ligase ( GCL ) catalyzes the rate - limiting step in the de novo synthesis of glutathione ( GSH ) . - pubmed
Although several studies suggest that this variation influences GSH content , its implication for GCLC expression remains unknown . - pubmed
These results provide evidence that interaction of the two variations can efficiently impair GCLC expression and thus suggest its involvement in the pathogenesis of diseases related to GSH metabolism . - pubmed
In vitro and in vivo evaluation of 64Cu - radiolabeled KCCYSL peptides for targeting epidermal growth factor receptor-2 in breast carcinomas . - pubmed
In this report , we tested the ability of a bacteriophage selected peptide KCCYSL , radiolabeled with 64Cu , to image EGFR-2 expressing breast tumors in vivo by positron emission tomography ( PET ) . - pubmed
PET / CT studies revealed successful tumor uptake of 64Cu - peptides at 2 hours postinjection . - pubmed
The optimization of bifunctional chelators improves the pharmacokinetic properties of the 64Cu - labeled GSG - KCCYSL peptide , which enables the selection of a suitable peptide homolog as a PET imaging agent for EGFR-2 expressing breast carcinomas . - pubmed
They were i ) Gram - negative , ii ) catalase positive , iii ) produced acid from glucose and maltose , iv ) tolerated 5 to 15 % NaCI v ) except MBRI 9 , showed pH tolerance in the range of 5.0 to 9.0 with optimum pH 7.0 to 8.0 v ) grew well at 30 degrees C and were able to grow in the range of 15 to 45 degrees C. EPA , an essential omega-3 fatty acid , was produced by these isolates in the range of 12 to 60 % at 30 degrees C. MBRI 12 was found to be a potential source as it produced 60 % EPA . - pubmed
Promising therapeutic effects have been reported in combination with DNA damaging agents , taxanes , targeted agents , death receptor agonists and hormonal therapies . - pubmed
The shells are made of phospholipids , surfactant , denatured human serum albumin , or synthetic polymer . - pubmed
Extracellular matrix ( ECM ) adhesion molecules consist of a complex network of fibronectins , collagens , chondroitins , laminins , glycoproteins , heparin sulfate , tenascins , and proteoglycans that surround connective tissue cells , and they are mainly secreted by fibroblasts , chondroblasts , and osteoblasts ( 7 ) . - pubmed
In all participants , measurement of height , weight , waist circumference , hip circumference , blood pressure systolic ( SBP ) and blood pressure diastolic ( DBP ) , resting heart rate ( RHR ) , blood glucose and lipids was carried out using standard methods . - pubmed
Low Density Lipoprotein - cholesterol ( LDL - c ) concentrations were calculated using the Friedwald formula . - pubmed
Triglycerides ( TG ) were significantly higher in urban than rural men ( 1.23 + - 0.39 mmol / L vs 1.17 + - 0.64 mmol / L , p = 0.017 ) . - pubmed
High Density Lipoprotein - cholesterol ( HDL - c ) levels were higher in rural compared to urban men ( 2.60 + - 0.10 35mmol / L vs 1.97 + - 1.14 mmol / L , p<0.001 respectively ) . - pubmed
However , total Cholesterol ( TC ) and LDL - c were significantly higher in urban than in rural men ( p<0.001 and p = 0.005 ) and women ( p<0.001 respectively . - pubmed
BACKGROUND AND PURPOSE : Ginsenoside - Rd is a receptor - operated calcium channel antagonist and has shown promise as a neuroprotectant in our phase II study . - pubmed
Synthesis of oxo - dihydrospiroindazole - based acetyl - CoA carboxylase ( ACC ) inhibitors is reported . - pubmed
Acylation of the resultant spirocyclic amines provided potent ACC inhibitors . - pubmed
In the present study we attempted to elucidate the mechanisms of glomerular injury induced by FK506 and the renoprotective effects of the angiotensin II receptor blocker telmisartan . - pubmed
RESULTS : FK506 induced ROS production via activation of NAD ( P ) H oxidase in the glomeruli . - pubmed
FK506 directly promoted NAD ( P ) H oxidase activity and accelerated production of ROS in hGECs . - pubmed
Conversely , cotreatment with telmisartan inhibited both NAD ( P ) H oxidase activity and production of ROS . - pubmed
CONCLUSION : These findings suggest that glomerular injury resulting from FK506 is caused by oxidative stress mediated by activation of NAD ( P ) H oxidase and that telmisartan exerts a renoprotective effect via antioxidative activity . - pubmed
Inhibition and induction of glutathione S - transferases by flavonoids : possible pharmacological and toxicological consequences . - pubmed
Many studies reviewed herein demonstrated the potency of some flavonoids to modulate the activity and/or expression of glutathione S - transferases ( GSTs ) . - pubmed
The effect of flavonoids on GSTs strongly depends on flavonoid structure , concentration , period of administration , as well as on GST isoform and origin . - pubmed
, acetaminophen , simvastatin , cyclophosphamide , cisplatine , polycyclic aromatic hydrocarbons , chlorpyrifos , acrylamide , and isocyanates ) , which are GST substrates , could have significant pharmacological and toxicological consequences . - pubmed
Although reasonable consumptions of a flavonoids - rich diet ( that may lead to GST induction ) are mostly beneficial , the uncontrolled intake of high concentrations of certain flavonoids ( e.g. - pubmed
The serum C3 levels of groups sensitized to TCE for 24 and 73h were 99.75 + - 1.45 and 93.28 + - 3.61g / ml , respectively , which were significantly lower than that ( 112.30 + - 9.10 g / ml ) of solvent control group ( P & lt ; 0.05 ) . - pubmed
Also The serum C4 levels of groups sensitized to TCE for 24 and 73 h were 34.63 + - 2.53 and 33.82 + - 2.76g / ml , respectively , which were significantly lower than that ( 43.87 + - 3.65 g / ml ) of solvent control group ( P & lt ; 0.05 ) . - pubmed
The serum IgA and IgM levels of groups sensitized to TCE and unsensitized to TCE for 24 and 72 h were significantly lower than those of solvent group ( P & lt ; 0.05 ) . - pubmed
as compared with unsensitized groups , the serum IgA levels of the groups sensitized to TCE for 24 and 72 h significantly decreased ( P & lt ; 0.05 ) . - pubmed
Flow cytometry showed that MIL induced a G2 / M arrest and apoptosis in a dose - dependent manner . - pubmed
Western blot demonstrated that MIL - induced G2 / M arrest was correlated with the inhibition of cyclin - dependent kinase 1 activity , including a remarkable decrease in cell division cycle ( cdc ) 2 synthesis , the accumulation of phosphorylated - Thr14 and decrease of phosphorylation at Thr161 of cdc2 . - pubmed
MIL also activated caspase 9 and caspase 3 , and significantly increased the ratio of Bax / Bcl-2 and stimulated the release of cytochrome c into cytosol , suggesting MIL induced apoptosis via mitochondrial apoptotic pathway . - pubmed
Neu2000 ( NEU ; 2 - hydroxy-5 -( 2,3,5,6 - tetrafluoro-4 - trifluoromethyl - benzylamino )- benzoic acid ) , a recently developed derivative of acetylsalicylic acid and sulfasalazine , potently protects against neuronal cell death following ischemic brain injury by antagonizing NMDA receptor - mediated neuronal toxicity and oxidative stress . - pubmed
These results suggest that NEU attenuates hypoxia - induced impairment of neuronal activity more potently than the antioxidant , trolox , and the NMDA receptor antagonists , ifenprodil and APV . - pubmed
Fifteen N - terminal amino acids were identified by Edman degradation , and 77 % of the rCPI primary structure was confirmed by mass fingerprint . - pubmed
When the purified CD13(high)CD133(+ ) cells were cultured at a low density with feeder cells in the presence of suitable growth factors and signaling inhibitors ( ALK inhibitor A-83 - 01 and ROCK inhibitor Y-27632 ) , individual cells gave rise to relatively large colonies . - pubmed
alpha - Synuclein oligomers induced by docosahexaenoic acid affect membrane integrity . - pubmed
Previously , we demonstrated that alpha - synuclein forms oligomeric species in the presence of docosahexaenoic acid and that these species are toxic to cells . - pubmed
Chromogranin A ( CgA ( CHGA ) ) is the major soluble protein co - stored and co - released with catecholamines and can function as a pro - hormone by giving rise to several bioactive peptides . - pubmed
The search strategies utilized keywords such as chromogranin A , vasostatins 1 and 2 , chromofungin , chromacin , pancreastatin , catestatin , WE14 , chromostatin , GE25 , parastatin , and serpinin and was supplemented by the screening of references from included papers and review articles . - pubmed
The analysis of the retrospective literature suggested that CgA and its several bioactive fragments exert a broad spectrum of regulatory activities by influencing the endocrine , the cardiovascular , and the immune systems and by affecting the glucose or calcium homeostasis . - pubmed
Proline , glutamic acid and leucine - rich protein-1 is essential for optimal p53 - mediated DNA damage response . - pubmed
PELP1 - depleted p53 ( wild - type ) breast cancer cells were less sensitive to various genotoxic agents including etoposide , camptothecin or gamma - radiation . - pubmed
QCASP3.2 did not exhibit any toxicity and appeared to accurately reflect the induction and inhibition of caspase activity by H2O2 and PACAP , respectively , both in cell lysates and in cultured neurons . - pubmed
L1 and Pn1 tumor cases displayed a significantly ( p & lt ; 0.05 ) decreased M1 and increased M2 polarization in the sinus of the lymph nodes . - pubmed
G3 cases presented a significantly ( p & lt ; 0.05 ) increased M2 polarization in the sinus compared to G2 cases . - pubmed
Photo - induced dynamics of the heme centers in cytochrome bc1 . - pubmed
The c1 - heme iron - ligand recombination time is 5.4 ps , in agreement with previous reports on homologous cytochromes . - pubmed
The different heme - responses illustrate the flexibility of the c1 - heme ligation in contrast to the more rigid b - heme binding , as well as the higher electronic reactivity of the b - hemes within the bc1 complex . - pubmed
Here we demonstrated that PINK1 - 53 is stabilized in the presence of enhanced Lys-63 - linked ubiquitination and identified TRAF6 - related NF - kappaB activation as a novel pathway involved in this . - pubmed
Twenty - five simulated roots from photoelastic resin were made and divided into 5 groups : CPC , cast post - and - core ; SP , screw post ; CF , carbon fiber post ; GF , glass fiber post ; and CGF , customized glass fiber post . - pubmed
After cementing CPC and SP posts with zinc phosphate cement , and CF , GF and CGF posts with resin cement , resin cores were made for groups 2 - 5 . - pubmed
The antisense BvCMO plants showed the decreased activity of GB synthesis from choline compared to wild - type ( WT ) plants which is well related to the suppressed level of BvCMO protein . - pubmed
[ Collagen and Taxifolin - based Material : Production and Properties ] . - pubmed
The possibility of producing the material based on collagen and biologically active polyphenol taxifolin was explored , and the properties of the material were studied . - pubmed
The data on the dynamics of the release of polyphenol chemically linked to collagen are represented , and the metal - reducing activity of polyphenol released from the gel is determined . - pubmed
It was shown that polyphenol released from the gel material exerts antioxidant and metal - reducing properties , suggesting that unoxidized polyphenol linked to collagen . - pubmed
We studied how several sub - types of vegetarian diets affect metabolic traits , including waist circumference , BMI , systolic blood pressure ( SBP ) , diastolic blood pressure , fasting blood glucose , total cholesterol ( TC ) , HDL , LDL , TAG and TC : HDL ratio , through both cross - sectional and longitudinal study designs . - pubmed
In the longitudinal follow - up , each additional year of vegan diet lowered the risk of obesity by 7 % ( 95 % CI 0 88 , 0 99 ) , whereas each additional year of lacto - vegetarian diet lowered the risk of elevated SBP by 8 % ( 95 % CI 0 85 , 0 99 ) and elevated glucose by 7 % ( 95 % CI 0 87 , 0 99 ) , and each additional year of ovo - lacto - vegetarian diet increased abnormal HDL by 7 % ( 95 % CI 1 03 , 1 12 ) , compared with non - vegetarians . - pubmed
In the cross - sectional comparisons , all sub - types of vegetarians had lower likelihoods of abnormalities compared with non - vegetarians on all metabolic traits ( P<0 001 for all comparisons ) , except for HDL and TAG . - pubmed
With proper management of TAG and HDL , along with caution about the intake of refined carbohydrates and fructose , a plant - based diet may benefit all aspects of the metabolic profile . - pubmed
A very low esterase activity and high water activity were observed when perlite , vermiculite and polyurethane were used as supports . - pubmed
Moreover , moisture retention was improved by mixing polyurethane and glass fiber , resulting in maximal biomass and esterase production . - pubmed
The application of hydrogen / deuterium exchange mass spectrometry ( HDX - MS ) to localize ligand binding sites in carbohydrate - binding proteins is described . - pubmed
Proteins from three bacterial toxins , the B subunit homopentamers of Cholera toxin and Shiga toxin type 1 and a fragment of Clostridium difficile toxin A , and their interactions with native carbohydrate receptors , GM1 pentasaccharides ( beta - Gal -( 1 3 )- beta - GalNAc -( 1 4 )[ alpha - Neu5Ac -( 2 3 )]- beta - Gal -( 1 4 )- Glc ) , Pk trisaccharide ( alpha - Gal -( 1 4 )- beta - Gal -( 1 4 )- Glc ) and CD - grease ( alpha - Gal -( 1 3 )- beta - Gal -( 1 4 )- beta - GlcNAcO ( CH2 ) 8CO2CH3 ) , respectively , served as model systems for this study . - pubmed
Overall , the results of this study suggest that HDX - MS can serve as a useful tool for localizing the ligand binding sites in carbohydrate - binding proteins . - pubmed
We describe the method to prepare neoglycoproteins from the conjugation of bovine serum albumin and pyridylaminated glycans . - pubmed
These pyridylaminated glycans can then be conjugated to bovine serum albumin via esterification with N - ( m - maleimidobenzoyloxy ) succinimide to form a neoglycoprotein , e.g. - pubmed
As a demonstration , we prepared High - mannose bovine serum albumin , which was immobilized on an activated glass slide . - pubmed
Evaluation of the gingival inflammation in pregnancy and postpartum via 25 - hydroxy - vitamin D3 , prostaglandin E2 and TNF - alpha levels in saliva . - pubmed
The aim of the present study was to evaluate saliva levels of 25 - hydroxy - vitamin D3 ( 25 ( OH ) D3 ) , prostaglandin E2 ( PGE2 ) and TNF - alpha ( TNF - alpha ) in pregnancy , postpartum and non - pregnant controls . - pubmed
25 ( OH ) D3 , PGE2 and TNF - alpha levels in the saliva samples were determined by enzyme - linked immunoassays . - pubmed
RESULTS : Saliva TNF - alpha and PGE2 levels were significantly lower and 25 ( OH ) D3 levels were significantly higher in the pregnant group than postpartum group ( p<0.0001 ) . - pubmed
Saliva TNF - alpha and 25 ( OH ) D3 levels were significantly higher and PGE2 levels were significantly lower in the control group than postpartum group ( p<0.0001 ) . - pubmed
Chloramphenicol resistance allows positive selection of clones after the first event , whereas a colorimetric assay based on the xylE gene provides a simple way to screen clones in which the second recombination event occurs . - pubmed
Abeta can induce astrocyte activation which give rise to produce reactive oxygen species ( ROS ) and cytotoxic cytokines and chemokines , and subsequently induce neuronal death . - pubmed
Four different transition metal complexes containing dipyridyl triazole ligands , namely [ Cu ( abpt ) 2Cl2 ] 2H2O ( 1 ) , [ Cu ( abpt ) 2 ( ClO4 ) 2 ] ( 2 ) , [ Co2 ( abpt ) 2 ( H2O ) 2Cl2 ] Cl2 4H2O ( 3 ) and [ Co2 ( Hbpt ) 2 ( CH3OH ) 2 ( NO3 ) 2 ] ( 4 ) have been designed , synthesized and further structurally characterized by X - ray crystallography , ESI - MS , elemental analysis , IR and Raman spectroscopy . - pubmed
However , RA subjects using current anti - TNF therapy ( n = 13 , disease activity score 1.98[1.8 - 2.2 ] ) have an almost 1.2 - fold higher ( 18 ) F - FDG uptake in the arterial wall compared to those using DMARDs ( but with previous anti - TNF therapy ) ( n = 10 , disease activity score 2.24[1.3 - 2.5 ] ) , which seemed to be predominantly explained by longer duration of their rheumatic disease in a multivariate linear regression analysis . - pubmed
Carbon capture and storage ( CCS ) provides a solution toward decarbonization of the global economy . - pubmed
Increase in dietary aflatoxin level resulted in the decreased villi height , villi height - to - crypt depth ratio ( VH : CD ) , villi surface area and apparent villi absorptive area , while it increased crypt depth , goblet cell count and lymphoid follicular diameter . - pubmed
BACKGROUND : Expression of the androgen receptor splice variant 7 ( AR - V7 ) is associated with poor response to second - line endocrine therapy in castration - resistant prostate cancer ( CRPC ) . - pubmed
Zepatier is a fixed - dose combination product coformulating MK-5172 [ grazoprevir ( GZR ) , 100 mg QD ] and MK-8742 [ elbasvir or ( EBR ) 50 mg QD ] : it combines highly potent inhibitors of the HCV NS3 / 4A protease and NS5A replication complex , respectively . - pubmed
Prototypical SOCs are formed of Orai1 proteins and are activated by the endo / sarcoplasmic reticulum Ca2 + sensor stromal interaction molecule 1 ( STIM1 ) . - pubmed
In Orai1-/- VSMCs , store depletion induced PLCbeta1 activity measured with the fluorescent phosphatidylinositol 4,5 - bisphosphate / inositol 1,4,5 - trisphosphate biosensor GFP - PLCdelta1 - PH , which was prevented by knockdown of STIM1 . - pubmed
Study outcomes included the incidence of bladder perforation and obturator jerk , decrease in hemoglobin and sodium levels , rates of re - coagulation and blood transfusion , TUR syndrome , and operation time . - pubmed
However , with a decrease in the hemoglobin and sodium levels , rates of re - coagulation and blood transfusion were greater in the monopolar arm . - pubmed
Pyk2 phosphorylation of VE - PTP downstream of STIM1 - induced Ca2 + entry regulates disassembly of adherens junctions . - pubmed
Here we investigated the role of stromal interaction molecule 1 ( STIM1 ) activation of store - operated Ca2 + entry ( SOCE ) in regulating AJ assembly . - pubmed
Pyk2 - induced tyrosine phosphorylation of VE - PTP promoted Src binding to VE - PTP , Src activation , and subsequent VE - cadherin phosphorylation and thereby increased the endothelial permeability response . - pubmed
Thus Pyk2 activation secondary to STIM1 - induced SOCE causes tyrosine phosphorylation of VE - PTP , and VE - PTP , in turn , binds to and activates Src , thereby phosphorylating VE - cadherin to increase endothelial permeability through disassembly of AJs . - pubmed
Our results thus identify a novel signaling mechanism by which STIM1 - induced Ca2 + signaling activates Pyk2 to inhibit the interaction of VE - PTP and VE - cadherin and hence increase endothelial permeability . - pubmed
Vitamin C , high sensitive C - reactive protein ( hs - CRP ) , soluble intercellular cell adhesion molecule 1 ( sICAM-1 ) , and soluble vascular cell adhesion molecule 1 ( sVCAM-1 ) were measured in a cohort of 598 RTR . - pubmed
A novel oxynitride compound , Pr4 - xCaxSi12O3 + xN18 - x , synthesized using a solid - state route has been characterized as a monoclinic structure in the C2 space group using Rietveld refinement on synchrotron powder X - ray diffraction data . - pubmed
TEM showed that the SWNTs with pyridine functionality more rapidly produced a more even distribution of copper seeds with a narrower size distribution ( 3 - 12 nm for py - US - SWNTs ) than those SWNTs with oxygen functional groups ( ca . - pubmed
In this study , a pair of water - soluble chiral 3,4 - ethylenedioxythiophene ( EDOT ) derivatives , ( R )- 2 '- hydroxymethyl-3,4 - ethylenedioxythiophene ( ( R )- EDTM ) and ( S )- 2 '- hydroxymethyl-3,4 - ethylenedioxythiophene ( ( S )- EDTM ) , were synthesized and electrodeposited on the surface of a glassy carbon electrode ( GCE ) via current - time ( I - t ) polymerization in an aqueous LiClO4 electrolyte . - pubmed
The results indicated that the ( R )- PEDTM / GCE sensor showed a higher peak current response toward the levo or ( S ) forms of the tested enantiomers , while the opposite phenomenon occurred for ( S )- PEDTM / GCE . - pubmed
AAs are vitally important for proper neurodevelopment , as they comprise the most abundant neurotransmitters in the brain and act as neurotransmitter precursors , nitric oxide donors , antioxidants , and neurotrophic factors , which induce synaptogenesis , neuronal proliferation , and migration . - pubmed
We conclude that OR and site 5 histidine substitutions have divergent phenotypic impacts and that cis interactions between the OR region and the site 5 region modulate pathogenic outcomes by affecting the PrP globular domain . - pubmed
2 - aminopurine suppresses the TGF - beta1 - induced epithelial - mesenchymal transition and attenuates bleomycin - induced pulmonary fibrosis . - pubmed
This compound significantly attenuated bleomycin ( BLM)-induced pulmonary inflammation , the EMT , and fibrosis . - pubmed
Collectively , we determined that 2 - AP could inhibit metastasis in vitro by suppressing the TGF - beta1 - induced EMT and could attenuate BLM - induced pulmonary fibrosis in vivo . - pubmed
In this study , we demonstrate that meta - tetra ( hydroxyphenyl ) chlorin ( mTHPC)-based photodynamic therapy combined with bevacizumab ( Avastin ) , an anti - VEGF neutralizing monoclonal antibody that blocks the binding of VEGF to its receptor , can enhance the effectiveness of each treatment modality . - pubmed
Enzyme - linked immunosorbent assay ( ELISA ) and immunohistochemistry ( IHC ) were performed to assess VEGF concentrations and microvessel density in the various treatment groups , and confocal imaging and high performance liquid chromatography ( HPLC ) analyses were used to measure the distribution and concentration of mTHPC in tumors . - pubmed
Immunostaining and ELISA analyses revealed a lower expression of VEGF in tumors treated with combination therapy of PDT followed by bevacizumab . - pubmed
UDG catalyzes the removal of uracil base from the enclosed dumbbell - shape DNA substrate to give an apyrimidinic site , at which the substrate oligonucleotide is cleaved by endonuclease IV . - pubmed
Part II : Phenolic compounds profile . - pubmed
Peptide polymerization relies on tyrosinase oxidation of tyrosine residues to Dopaquinones , which rapidly form cysteinyldopa - moieties with free thiols from cysteine residues , thereby linking unimers and generating adhesive polymers . - pubmed
Chromatographic separation coupled with high - resolution mass spectrometry revealed beta - galactosidase degradation of several beta - linkage - containing BMOs and production of novel oligosaccharides that ranged in size from 5 to 11 monosaccharide units containing several galactose repeating units and N - acetylhexosamine at their reducing ends . - pubmed
Fourier transfer infrared ( FTIR ) , Nuclear Magnetic Resonance ( NMR ) and thermogravimetric ( TG ) analysis revealed that DOPO and DPSD are linked to EGTO by a covalent bond . - pubmed
Olopatadine , a topical H1 antagonist used in the treatment of ocular allergies , may interfere with the humoral regulation of the iris arteriolar tone . - pubmed
Thermal and chemical unfolding of lysozyme in the presence of guanidineHCl denaturant is a model system to compare the conventional 2 - state model of protein unfolding with the multi - state Zimm - Bragg theory . - pubmed
Lysozyme unfolding is associated with a large endothermic enthalpy that decreases significantly upon addition of guanidineHCl . - pubmed
The Zimm - Bragg theory provides the protein cooperativity parameter , the average length of independently folding protein domains , and the Gibbs free energy of unfolding of individual amino acid residues . - pubmed
GuanidineHCl binding to lysozyme is exothermic and counteracts the endothermic unfolding enthalpy . - pubmed
The number of bound denaturant molecules is determined from the decrease in enthalpy and is extrapolated to the guanidineHCl - to - amino acid stoichiometry at complete lysozyme unfolding . - pubmed
Two alkaline - earth coordination compounds , [ Ba ( C8H4N4O2 )( H2O ) 4 ] n , ( I ) , and [ Sr ( C8H4N4O2 )( H2O ) 3 ] n , ( II ) , from the one - pot hydrolysis transformation of benzoyl chloride and the in situ self - assembled [ 2 + 3 ] cyclo - addition of nitrile are presented . - pubmed
Protective effect of proton pump inhibitors and potassium competitive acid blockers against post - gastric endoscopic submucosal dissection bleeding : a single - center , propensity score - matched analysis . - pubmed
OBJECTIVES : Both potassium - competitive acid blockers ( P - CABs ) and proton pump inhibitors ( PPIs ) are known to be protective against bleeding after gastric endoscopic dissection ( ESD ) for early gastric cancers . - pubmed
The aim was to compare the effect of PPI and P - CAB treatment against bleeding after gastric ESD . - pubmed
Reconstitution of a Ca2 + - transporting ATPase system from triton X-100 - solubilized sarcoplasmic reticulum . - pubmed
L - DOPA , dopamine , and tyrosine were found to be good substrates for this enzyme . - pubmed
Vmax = 14.1 , 8.1 , and 6.1 mumoles O2 / min / mg protein for each substrate , respectively . - pubmed
The relationship between the pulmonary toxicity of 3 - methylindole ( 3MI , skatole ) and the mixed - function oxidase ( MFO ) system was investigated . - pubmed
Results indicate that the MFO system is one of the pathways involved in the metabolism of 3MI and that pulmonary toxicosis results from metabolism of 3MI by this enzyme system . - pubmed
The inability of a peptide to substitute for the requisite amino acid was not due to the absence of cellular peptidases or to toxicity of the nonutilized peptides . - pubmed
In the bound state , the enzyme exhibits a greater affinity for glutamate , but a lower affinity for oxalacetate . - pubmed
A similar effect was elicited by a D - stereoisomer of propranolol with no beta - adrenoceptor blocking activity . - pubmed
In the observations reported here , the HMB45 - defined antigen was identified in another pigmented tissue , the retinal pigment epithelium ( RPE ) . - pubmed
Pre - treatment of deparaffinized tissue sections with neuraminidase completely eliminated HMB45 immunoreactivity , suggesting that the antigen(s ) recognized is a sialated glycoconjugate . - pubmed
A wide series of restriction enzymes with a range of specificities was used to investigate the interaction with DNA of Ru ( II )- DMSO complexes with anticancer activity . - pubmed
While cis - RuCl2 ( DMSO)4 was almost completely inactive , treatment of pBR 322 DNA with trans - RuCl2 ( DMSO)4 protected G rich sequences from cutting of specific restriction endonucleases , indicating a preferential interaction with adjacent guanines . - pubmed
The possible involvement of cyclic AMP in the control of the PEPCK activity of the primary renal cells was indicated by the stimulatory effects of 8 bromocyclic AMP , isobutyl methylxanthine ( a cyclic AMP phosphodiesterase inhibitor ) , and forskolin ( an activator of adenylate cyclase ) . - pubmed
Phorbol 12 - myristate 13 - acetate ( TPA ) , which activates protein kinase C , was inhibitory . - pubmed
Some of the former co - expressed HLA - DR antigens but not CD1a antigens . - pubmed
AT II , elymoclavine and bromocryptine were applied alone . - pubmed
Elymoclavine potentiates the effect of AT II during exploratory behaviour . - pubmed
The effects of the drugs tested on the exploratory behaviour most probably result from the interaction between AT II receptors , dopamine receptors and through GABA - ergic neurotransmission , in the respective brain zones responsible for behaviour . - pubmed
An attempt was made to predict clinical variants of a course of DTG using HLA - antigens . - pubmed
Gel column chromatography revealed that the pituitary ir FGF was present mainly as high molecular weight forms , with only a small proportion as the basic 146 amino - acid peptide . - pubmed
In order to avoid cross - reactions with other members of the keratin multigene family , appropriately taylored 35S - labeled cDNA probes comprising the low and non - homologous 3 ' coding and noncoding domains of the mRNAs were used for in situ hybridization to tissue sections . - pubmed
It was shown that IDF45 acted in the G1 phase of the cell cycle . - pubmed
Furthermore , IDF45 inhibited the early stimulation of RNA synthesis induced by alpha - globulin . - pubmed
By comparing amino acid sequences of Id+ and Id- anti - alpha(1 - ---6)dextrans , the major idiotype was assigned to residues in VH CDR3 , with a possible contribution from VH CDR2 , a conclusion supported by the hapten inhibition results . - pubmed
Initially , both groups had a similar body mass index , resting heart rate and blood pressures , blood glucose and hemoglobin A1 . - pubmed
Although total cholesterol was found to be reduced by 13 % for the exercise group ( P less than 0.03 ) and 11 % for the controls ( P less than 0.01 ) , a 15 % decrease ( P less than 0.03 ) in high - density lipoprotein ( HDL ) was observed for the control group , only . - pubmed
In this report the p beta H alpha-5 clone was used to investigate whether patients with two different variants of Tay - Sachs disease also lack the alpha - chain message . - pubmed
In the acute phase there was a tendency to a negative correlation of erythrocyte sedimentation rate and of C - reactive protein with serum cholesterol and/or HDL cholesterol . - pubmed
Both doses of 5 - hydroxytryptamine elicit a strong release of GH , the effect being dose - dependent . - pubmed
In pituitaries perifused with 5 - hydroxytryptamine ( 100 microM during 115 min or 1 , 10 and 100 microM during 15 min ) , a significant release of GH was also observed . - pubmed
These results suggested that 5 - hydroxytryptamine may stimulate GH secretion through a direct pituitary action . - pubmed
Attempts at histochemical localization of estrogen receptor with anti - steroid antibody or some fluoresceinated estrogens have given unacceptable sensitivities and specificities when compared with biochemical methods or clinical response . - pubmed
Cysteine specific activity was quantitated , and found to decline rapidly after injection of the labeled amino acid : less than 0.1 % of the injected label remained as free cysteine in the hypothalamus 30 min post injection . - pubmed
Incorporation of label into SRIF-14 and SRIF-28 reached maximum values 8 h post injection , compared with a labeling maximum at 1 - 2 h for acid - precipitable protein . - pubmed
Within 2 h after [ 35S ] cysteine injection , nearly half of the total labeled hypothalamic SRIF appeared in the medial basal hypothalamus ; 24 - h post injection this percentage reached approximately 75 % . - pubmed
Colchicine administration dramatically reduced the appearance of labeled SRIF in medial basal hypothalamus , but had no apparent effect on the total incorporation of [ 35S ] cysteine into SRIF-14 , SRIF-28 , or acid - precipitable protein , or on radioimmunoassayable SRIF levels . - pubmed
[ ATPase activity and ATP - dependent accumulation of Ca2 + in skeletal muscle nuclei . - pubmed
An addition of Ca2 + significantly increased the activity of nuclear ATPase . - pubmed
The changes in Ca - ATPase activities and in the ATP - dependent uptake of 45Ca induced by denervation and electric stimulation were found to be synchronous and unidirectional . - pubmed
The effects of triethyltin bromide on red cell and brain cyclic AMP - dependent protein kinases . - pubmed
Additions of 25 - 500 microM triethyltin to red cell ghosts resulted in enhanced phosphorylation of ghost proteins . - pubmed
Brief exposure of the partially purified enzymes to triethyltin , followed by DE52 chromatography , resulted in elution profiles for regulatory and catalytic subunits that were similar to the profile resulting after cyclic AMP activation . - pubmed
The inhibition was non - competitive with respect to both histone and ATP . - pubmed
When intact red cells were subjected to brief exposure with triethyltin , enhanced phosphorylation of certain membrane proteins occurred , suggesting that the activation of the cyclic AMP protein kinases by triethyltin may be physiologically significant . - pubmed
Enzymatic activity of the protein was detectable only in assays containing highly purified gamma - glutamyl phosphate reductase , the second enzyme of the proline pathway . - pubmed
Plots of gamma - glutamyl kinase activity as a function of glutamate concentration were sigmoidal , with a half - saturation value for glutamate of 33 mM , whereas plots of enzyme activity as a function of ATP concentration displayed typical Michaelis - Menten kinetics with a Km for ATP of 4 X 10(-4 ) M. Enzyme activity was insensitive to the glutamate analog L - methionine - DL - sulfoximine , but ADP was a potent competitive inhibitor . - pubmed
These results indicated that the only major difference was that the enzyme from the 3,4 - dehydroproline - resistant strain was 100 - fold less sensitive to feedback inhibition by proline . - pubmed
These findings strongly suggest that the combination of 5 - Fu and HuIFN - alpha or 5 - Fu alone is selectively effective on the neoplastic cells of HSG cell population but not on the non - neoplastic cells . - pubmed
The enzyme was maximally active at pH 7.6 in the presence of 4 mM calcium . - pubmed
The enzyme was labile in aqueous solutions and storage at 4 degrees C and --20 degrees C resulted in considerable loss of activity , unless glycerol ( 50 % v / v ) was added to the solution . - pubmed
The behaviour of blood lead ( PbB ) and of some indicators of effect ( erythrocyte protoporphyrin IX ( EP ) , delta - aminolaevulinic acid dehydratase activity of erythrocytes ( ALAD ) , and urinary delta - aminolaevulinic acid ( ALAU ) ) were studied in subjects who had ceased working with inorganic lead for at least one year . - pubmed
The relation between EP and PbU - EDTA shows that , corresponding levels of chelatable lead , the values of the erythrocyte metabolite are identical in the two groups . - pubmed
Considering the EP - PbB relation , however , at the same PbB levels the protoporphyrin values appear distinctly more altered in the subjects with past exposure . - pubmed
Similar results were obtained from a study of the relations between ALAD and PbU - EDTA and between ALAD and PbB. - pubmed
On the basis of the results obtained it is concluded that in subjects with past exposure , EP and ALAD can be used in establishing the persistence and extent of an " active deposit " of lead in the organism , while PbB is of very limited use . - pubmed
The present results showed that ANF enhanced the salivation induced by pharmacological agents which stimulate phosphatidylinositol hydrolysis . - pubmed
These effects of ANF may be probably related to the activation of the non - guanylate cyclase coupled receptor which has been associated with phosphatidylinositol turnover . - pubmed
Leukocytic DNA was extracted from the blood of 93 patients after which the region containing two mutation points coding amino acid residues 112 and 158 was amplified by using the polymerase chain reaction ( PCR ) . - pubmed
The alpha - tocopherol - binding protein did not bind oleate as assessed by direct radiolabeled fatty acid binding and fluorescence enhancement assay . - pubmed
The Western blot , ligand affinity , molecular size , and amino acid analysis data suggest that the alpha - tocopherol - binding protein is different from the cytosolic fatty acid - binding protein and that it may be involved in intracellular transport and metabolism of alpha - tocopherol . - pubmed
The selective tissue kallikrein inhibitor CH-694 ( but not the selective plasma kallikrein inhibitor CH-684 ) caused highly significant falls in airways resistance when it was administered at 10 mg / kg intraperitoneally 15 min before and 90 min after challenge . - pubmed
BACKGROUND : Taxol is a new anticancer drug that acts as a tubulin polymeration enhancer . - pubmed
Reaction of 7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole with the ( Ca2 + + Mg2 +)- ATPase protein of sarcoplasmic reticulum at low temperature . - pubmed
Modification of the ( Ca2 + + Mg2 +)- ATPase protein of rabbit skeletal sarcoplasmic reticulum ( SR ) with 7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole , NBD - Cl , at 4 degrees C for 5 min caused a 63 % loss of the Ca ( 2 +)-dependent ATPase activity when 1 mol of the adenine analog was incorporated per 10(5 ) g of protein . - pubmed
MgATP protected the ATPase activity at 4 degrees C and 25 degrees C but there was little change in the extent of labeling at 4 degrees C suggesting that changes in the fluidity of the lipid moiety made different sites on the ATPase protein accessible to the reagent . - pubmed
At 4 degrees C , addition of sodium deoxycholate enhanced the inactivation ( 6 % ATPase activity remained ) but the labeling of the SR - ATPase protein did not increase significantly . - pubmed
Incubation with MgATP prior to solubilization with deoxycholate resulted in the protection of the Ca ( 2 +)- ATPase activity and only a small decrease in the labeling occurred . - pubmed
At 25 degrees C , a similar pattern was found with deoxycholate but the loss of ATPase activity was less dramatic and the extent of labeling by NBD - Cl was greater than that at 4 degrees C. MgATP induced changes in the conformation of the ATPase protein protecting essential cysteine residues while shifting the reaction of NBD - Cl with the ATPase protein to non - essential sites in the absence or presence of deoxycholate . - pubmed
An analysis of tryptic digests of the NBD - ATPase protein showed that MgATP shifted the labeling from the A2 subfragment to the A1 subfragment in the absence of deoxycholate and from the A1 subfragment to the A2 subfragment in the presence of deoxycholate . - pubmed
The reagent , NBD - Cl , can distinguish between different temperature dependent conformational states of the ATPase protein . - pubmed
SN2 reaction of the known cyclopentenyl units 12 and 13 with 2 - haloadenines under basic conditions gave the protected carbocyclic nucleosides 14b , c and 15b , c , respectively . - pubmed
No evidence for association of dopamine D2 receptor variant ( Ser311 / Cys311 ) with major psychosis . - pubmed
We investigated a variant of the dopamine D2 receptor gene ( Ser311 / Cys311 substitution ) in Caucasian patients with schizophrenia ( n = 273 ) , delusional disorder ( n = 62 ) , bipolar I affective disorder ( n = 63 ) , and controls ( n = 255 ) . - pubmed
However , anti - ANT antibody can inhibit the ADP / ATP exchange of heart mitochondria and be organ - specific . - pubmed
An HPLC method is described for simultaneously obtaining the enantiomeric ratio of three amino acids ( aspartic acid , serine , and glutamic acid ) from dental collagen , with a view to using this information for estimating age at death . - pubmed
Pancreastatin ( PST ) , a recently discovered regulatory peptide derived from chromogranin A , has been shown to have a glycogenolytic effect in the hepatocyte that is mediated by increasing intracellular calcium . - pubmed
Since cerebral metabolic activity reflects regional brain work for all neurotransmitter systems , imaging metabolism directly with fluorodeoxyglucose and indirectly with blood flow and hemoglobin oxygen saturation can provide information about the functional neuroanatomy of a deficit in individual patients and allow patients to be grouped into more homogeneous subgroups for intensive study . - pubmed
PURPOSE : Acute anterior uveitis is strongly associated with the HLA - B27 antigen and triggered by the involvement of some external factors . - pubmed
White phosphorus affected several plasma constituents , most notably elevated aspartate aminotransferase , blood urea nitrogen , lactate dehydrogenase , and alanine aminotransferase . - pubmed
The presence of a methyl group on the piperazine moiety decreased the pharmacodynamic contribution to the convulsant activity by severalfold , and the ratios of concentrations of the FQs in cerebrospinal fluid ( CSF ) to concentrations of unbound FQs in plasma varied from about 5 to 75 % as a function of lipophilicity . - pubmed
This effect was evidenced by a reduction of cell viability in the MTT assay , G1 arrest and significant apoptosis upon flow - cytometric analysis , presence of apoptotic bodies , formation of DNA ladders upon gel electrophoresis and inhibition of viral early gene expression by RT - PCR and Western blot . - pubmed
NSAIDs cause gastrointestinal damage by 2 independent mechanisms : a topical effect , which is pH and pKa related , and a systemic effect mediated by cyclooxygenase ( COX ) inhibition with a reduction in prostaglandin synthesis . - pubmed
Among the strategies used to decrease the risk of ulcer development are : ( i ) the use of analgesics other than NSAIDs ; ( ii ) use of the lowest possible dosage of NSAID ; ( iii ) the use of a COX-2 selective NSAID ; ( iv ) the use of low doses of corticosteroids instead of NSAIDs ; ( v ) avoidance of concomitant use of NSAIDs and corticosteroids ; and ( vi ) use of preventive therapy . - pubmed
In an attempt to reduce the incidence of NSAID - induced gastrointestinal lesions , the following approaches have been proposed : ( i ) use of the prostaglandin analogue misoprostol , which is an antiulcer drug which has been proven to be as effective in the prevention of NSAID - induced gastric and duodenal ulcers as in the reduction of serious upper gastrointestinal complications ; ( ii ) histamine H2 receptor antagonists ( H2 antagonists ) , e.g. - pubmed
ranitidine , cimetidine and famotidine , which are useful in the prevention of NSAID - induced duodenal ulcers during long term treatment , but not in the prevention of NSAID - induced gastric ulcers ; ( iii ) proton pump inhibitors , e.g omeprazole , and pantoprazole , whose efficacy in preventing NSAID - associated ulcers has been recently demonstrated ; and ( iv ) barrier agents , e.g. - pubmed
Incubation of guinea - pig peritoneal macrophages with cholera toxin ( CT ) or aluminium hydroxide gel ( Al - gel ) released a significantly higher activity of lactate dehydrogenase than did commercial natural CTB ( CTB ) or rCTB . - pubmed
Intraintestinal or intramuscular administration of CT , CTB or Al - gel caused severe histopathological reactions . - pubmed
Interactions of human fibrinogens with factor XIII : roles of calcium and the gamma ' peptide . - pubmed
Factor XIII binds fibrinogen gamma ( A )/ gamma ' approximately 20 - fold more tightly than fibrinogen gamma ( A )/ gamma ( A ) , and the interaction with fibrinogen gamma ( A )/ gamma ' ( but not fibrinogen gamma ( A )/ gamma ( A ) ) is accompanied by a significant release of Ca ( 2 + ) . - pubmed
Because of its induction during carbohydrate and complete starvation ( incubation in tap water ) and CdCl ( 2 ) and bile salts stresses , one of these proteins ( Gls24 ) was qualified as a " general stress protein " and was analyzed at the molecular level . - pubmed
A mutation in the gls24 gene led to a severe reduction of growth rate and reduction of survival against 0.3 % bile salts in the 24 - h - starved cells compared to the wild - type strain . - pubmed
Three of these sequences map in genes coding for L - lactate dehydrogenase , lipoamide dehydrogenase , and pyruvate decarboxylase , all involved in pyruvate metabolism . - pubmed
The expression of P - selectin and fibrinogen binding was not affected by bezafibrate therapy . - pubmed
Under voltage clamp , the initial rate of increase in [ Ca2 +]( i ) in guard cells is sensitive to the extracellular divalent concentration , suggesting a close interaction between the permeant ion and channel gating . - pubmed
Low Na superfusion significantly suppressed the relaxation and decreased the rest potentiation in the myocardial preparations all ages . - pubmed
The PCR results and the ability of 1 - oleoyl - LPA to mimic these drugs ( also with respect to their susceptibility to antagonists ) suggest that the release - inhibiting receptor may be an edg - encoded lysophospholipid receptor . - pubmed
For each subject , blood samples were measured for complete blood count , reticulocyte count , percentage of hypochromic red cells ( % HRC ) , serum ferritin , serum iron , transferrin saturation ( TS ) , serum erythropoietin ( sEpo ) , C - reactive protein ( CRP ) , and sTfR. - pubmed
Our study showed that sTfR levels quantitatively reflect the integrated effects of iron availability , iron reserves , and erythropoietic stimulation . - pubmed
Pro - aqualysin I ( 38kDa ) as inactive protein was secreted into the medium of shake - flask cultures at a concentration of 1g / L. It was isolated from the culture supernatant by an ammonium sulfate precipitation and one - step anion exchange chromatography in a nearly pure form and was autoproteolytically activiated by heat treatment . - pubmed
Paper electrophoresis as a quantitative method : measurement of alpha and beta lipoprotein cholesterol . - pubmed
Phosphatidylinositol - specific phospholipase C ( PLC ) enzymes catalyze hydrolysis of phosphatidylinositol 4,5 - bisphosphate ( PIP2 ) generating diacylglycerol ( DAG ) and inositol 1,4,5 - triphosphate ( IP ( 3 ) ) . - pubmed
In the case of hexahistidine ( 6 - His)-tagged PLC beta the purification involves two steps , affinity chromatography with Ni - NTA agarose followed by heparin Sepharose chromatography . - pubmed
6 - His - tagged PLC epsilon can be purified in a single step with nickel nitrilotriacetic acid - agarose ( Ni - NTA ) affinity chromatography . - pubmed
Other bisphosphonates ( pamidronate , alendronate , and incadronate ) did not increase alkaline phosphatase activity in either cell line . - pubmed
These data suggest that clodronate acts directly on mesenchymal cells to enhance osteoblast differentiation , and this effect may be partly expressed through inhibition of protein Tyr phosphatase and/or Ser / Thr phosphatase activity . - pubmed
The title compound crystallizes in space group P21 / c with two molecules in the asymmetric unit , both of which exhibit disorder in one of their thiophene rings . - pubmed
Maximum electrode response was found in phosphate buffer at pH 8.0 and 35 degrees C. The reproducibility of the enzyme electrode was also tested by using standard phenol solutions ( 0.005 microM ) . - pubmed
Thirty female Small East African goat kids at an average age of 5 months were initially treated with albendazole orally ( 5 mg kg(-1 ) body mass ) and randomly assigned into one of two groups : group I were fed UMB prepared using a cold process and group II kids ( controls ) received no block supplementation ( NBS ) . - pubmed
These results suggest that the accumulation of amino acid substitutions in the HA protein during evolution promoted irreversible structural changes and therefore that antigenic changes in the H3HA protein may not be limited . - pubmed
None of the gonadoblastomas showed immunoreactivity with antibodies against steroid hormone receptors and against testosterone and estradiol , respectively . - pubmed
Removal of both low - molecular - weight components and histones increased the yield of 8 - oxo - dG 46 - fold and the yield of strand breaks 43 - fold . - pubmed
Removal of only the histones thus leads to a two times greater increase in the yield of strand breaks compared to 8 - oxo - dG. - pubmed
These results suggest that in intact cells , 8 - oxo - dG is preferentially formed in histone - free structures of chromatin , indicating a larger role for the indirect effect of radiation in the formation of 8 - oxo - dG than in the formation of strand breaks . - pubmed
Enzymically hydrolysed DNA was subjected to HPLC - MS / MS analysis , which demonstrated that the phiR1 - 37 genome is composed of DNA in which thymidine ( T ) is & gt;99 % replaced by deoxyuridine ( dU ) . - pubmed
N - terminal amino acid sequences of four major structural proteins did not show any similarity to ( viral ) protein sequences in databases , indicating that close relatives of phiR1 - 37 have not yet been characterized . - pubmed
GSTM1 ( - ) smokers had a significantly higher level of plasma DNA adducts and leukocytes level of 8 - OHdG than their GSTM1 ( + ) counterparts ( P & lt ; 0.01 ) . - pubmed
Only the GSTM1 ( - ) subgroup was the one that , under FPP treatment , increased lymphocyte 8 - OHdG ( P & lt ; 0.01 ) . - pubmed
In spite of the nonsulfidic conditions and abundant reactive iron ( III ) commonly found in mobile tropical deltaic muds , genes encoding dissimilatory sulfite reductase ( dsr ) were successfully amplified from the upper approximately 1 m of coastal deposits sampled along French Guiana and in the Gulf of Papua . - pubmed
The spatial and temporal distribution of dsr sequences strongly supports the contention that the sulfate - reducing prokaryote communities in mobile mud environments are cosmopolitan and stable over a period of years . - pubmed
Recently , association of an interleukin-1A promoter polymorphism ( -889 , thymine / thymine ( T / T ) ) with Alzheimer ' s disease was reported , suggesting that this cytokine may play an important role in disease development . - pubmed
Indirect staining of antibodies using fluorescein isothiocyanate indicated CRA were concentrated mainly around epidermal and mesophyll cells in compatible host ( T-78 ) . - pubmed
This study aimed to evaluate a small - molecule PAI-1 inhibitor ( PAI-039 ; tiplaxtinin ) in a rodent stenosis model of venous thrombosis in a two - phase experiment . - pubmed
PAI-039 treatment was also associated with significantly increased return of inferior vena cava blood flow four days post - thrombosis versus controls ( p & lt ; 0.05 ) . - pubmed
RV09 , a novel resveratrol analogue , inhibits NO and TNF - alpha production by LPS - activated microglia . - pubmed
Moreover , RV09 attenuated the expression of mRNA and protein of tumor necrosis factor - alpha ( TNF - alpha ) in a concentration - dependent manner . - pubmed
In the summary , these data suggest that , by blocking IkappaBalpha phosphorylation and degradation , RV09 acts to suppress the LPS - induced NO and TNF - alpha production in microglia , and this effect was mediated , at least in part , by inhibiting the generation of ROS . - pubmed
Insulin secretion was estimated by acute insulin response ( AIR ) during an intravenous glucose - tolerance test ( IVGTT ) , and the disposition index was calculated as AIR x M(FFM ) . - pubmed
M(FFM ) was higher in MHO who exhibited lower waist circumference , blood pressure ( BP ) , triglycerides , FFA , insulin levels , and higher high - density lipoprotein ( HDL ) cholesterol compared to insulin - resistant obese . - pubmed
We found that the formation of yolk sac hemogenic endothelium and its hematopoietic potential were significantly impaired in the absence of retinoic acid ( RA ) signaling , and could be restored in RA - deficient ( Raldh2(-/- ) ) embryos by provision of exogenous RA in utero . - pubmed
BACKGROUND : Understanding how androgen receptor ( AR ) function is modulated by exposure to steroids , growth factors or small molecules can have important mechanistic implications for AR - related disease therapies ( e.g. - pubmed
This study compares Breast Cancer 1 ( BRCA1 ) and excision repair cross complementation group 1 ( ERCC1 ) expression as predictive markers and evaluates the in vitro enhancement of platinum sensitivity using targeted agents in sporadic ovarian cancer ( OC ) . - pubmed
In summary , this study shows that low BRCA1 and ERCC1 expression correlate with improved survival in advanced OC and HDAC inhibition induces synergistic cytotoxicity with platinum in vitro . - pubmed
Similar to the endocytosis motor dynamin , Drp1 uses GTP hydrolysis to power constriction of the outer mitochondrial membrane and ultimately mitochondrial division . - pubmed
Dynamin phosphorylation in its unique C - terminal proline - rich domain interferes with binding of accessory proteins that induce membrane curvature and inhibits clathrin - mediated endocytosis . - pubmed
We describe brain calcifications secondary to hypoparathyroidism in family members with APS and compare clinical manifestations , the extent of brain calcifications on CT scans and the result of PET - FDG scans . - pubmed
The micelle properties of PAsp ( OBzl )- b - PVP diblock copolymer were extensively studied by dynamic light scattering ( DLS ) , fluorescence spectroscopy , and transmission electron microscopy ( TEM ) . - pubmed
Nucleotide binding by Lhs1p is essential for its nucleotide exchange activity and for function in vivo . - pubmed
Both proteins have the characteristic domain structure of the Hsp70 family consisting of a conserved N - terminal nucleotide binding domain and a C - terminal substrate binding domain . - pubmed
Kar2p is a canonical Hsp70 whose substrate binding activity is regulated by cochaperones that promote either ATP hydrolysis or nucleotide exchange . - pubmed
Lhs1p is a member of the Grp170 / Lhs1p subfamily of Hsp70s and was previously shown to function as a nucleotide exchange factor ( NEF ) for Kar2p . - pubmed
Analysis of the nucleotide requirement of these functions demonstrates that nucleotide binding to Lhs1p stimulates the interaction with Kar2p and is essential for NEF activity . - pubmed
In contrast , Lhs1p holdase activity is nucleotide - independent and unaffected by mutations that interfere with ATP binding and NEF activity . - pubmed
Thus , Lhs1p - dependent nucleotide exchange activity is vital for ER protein biogenesis in vivo . - pubmed
In the carotenoid biosynthesis pathway , phytoene synthase ( PSY , EC 2.5.1.32 ) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate ( GGPP ) to phytoene and has been shown to be a rate - limiting enzyme for the synthesis of carotenoids . - pubmed
Transcript levels of carotenoid biosynthetic genes in the CsPSY overexpressed plants remained unaltered except that PDS and ZDS showed a minor increase . - pubmed
Decreasing Mg ( 2 + ) ATPase activity confirms that the age - related loss of PtdSer asymmetry is likely due to loss of flippase activity , more prominent in APP / PS-1 brain . - pubmed
Two - site sandwich ELISA on SDS- and FA - soluble APP / PS-1 brain fractions were conducted to correlate Abeta ( 1 - 40 ) and Abeta ( 1 - 42 ) levels with age - related trends determined from the AV , NBD - PS , and Mg ( 2 + ) ATPase assays . - pubmed
ELISA revealed a significant increase in both SDS- and FA - soluble Abeta(1 - 40 ) and Abeta ( 1 - 42 ) with age , consistent with PtdSer and flippase assay trends . - pubmed
Lastly , because PtdSer exposure is affected by pro - apoptotic caspase-3 , levels of both latent and active forms were measured . - pubmed
The model was used to fit the permeation profile of lidocaine formulated in oil - in - water ( Type I ) and water - in - oil ( Type II ) lecithin - linker microemulsions . - pubmed
For Type II systems , the increase in lidocaine concentration in the skin was linked to the increase in skin - donor partition . - pubmed
Donor - acceptor pairs of probes were attached to three site - directed di - Cys mutants of RLC , each having one Cys at position 129 in the C - terminal lobe and the other at position 2 , 3 , or 7 in the N - terminal PD . - pubmed
Protocol for 18F - FDG quantification in PET - CT whole - body exams . - pubmed
Soy isoflavones did not affect DBP [ -0.13 ( 95 % confidence interval -1.03 to 0.78 ) mmHg , P = 0.78 ; heterogeneity P = 0.20 , fixed effect model ] . - pubmed
The induction of apoptosis by Ca ( 2 +)-mobilizing agents results in caspase activation , which is similar to what is seen with other inducers of apoptosis . - pubmed
In addition , Ca ( 2 +)-dependent proteases , such as calpain and a Ca ( 2 +)- dependent nuclear scaffold - associated serine protease , are also activated by Ca ( 2 + ) signalling in some cell types where they appear to be involved in alpha - fodrin and lamin beta cleavage , respectively . - pubmed
terreus the cis - aconitate decarboxylase gene is part of an itaconate acid related gene cluster including genes encoding two pathway specific transporters and a Zinc finger protein . - pubmed
Cu ( II )-catalyzed olefin migration and Prins cyclization : highly diastereoselective synthesis of substituted tetrahydropyrans . - pubmed
Metal - ligand complexes of Cu ( OTf )( 2 ) with an appropriate bisphosphine ligand have been shown to effectively catalyze the formation of substituted tetrahydropyrans via a sequential olefin migration and Prins - type cyclization . - pubmed
3alpha - Hydroxysteroid dehydrogenase and related enzymes play important roles in the metabolism of endogenous compounds including androgens , corticosteroid , prostaglandins and bile acids , as well as drugs and xenobiotics such as benzo ( a ) pyrene . - pubmed
HT decisions included continued medical treatment ( 22 % ) , percutaneous coronary intervention ( PCI ; 22 % ) , coronary - artery bypass grafting ( CABG ; 34 % ) or further investigations such as pressure wire studies , dobutamine stress echo or cardiac magnetic resonance imaging ( 22 % ) . - pubmed
In the present studies , the effects of oil rich in EPA on gene expression and activation of nuclear receptors was examined and compared with other omega3 - PUFAs . - pubmed
Serum calcium level , fasting blood glucose level ( FBG ) , hexokinase activity , glucose-6 - phosphatse and fructose 1,6 bisphosphatase activity were measured to establish a relevant correlation between vitamin D supplementation and hyperglycemia in T2DM . - pubmed
The activity of the glucose metabolic enzymes was also assayed in diabetic group and was found to be deviated from control group ; hexokinase ( 0.0241 + - 0.014 mug / mg / ml ) FBPase ( 0.433 + - 0.002 mug / mg / ml ) and G6Pase ( 0.918 + - 0.02 mug / mg / ml ) . - pubmed
However , the activity of these enzymes returned to near control values with hexokinase activity reaching 0.717 + - 0.003 mug / mg / ml on vitamin D3 supplementation . - pubmed
Inhibitor and substrate interactions with equilibrative nucleoside transporter 1 ( ENT1 ; SLC29A1 ) are known to be affected by cysteine - modifying reagents . - pubmed
A previous study from our laboratory established Cys222 in transmembrane ( TM ) 6 as the residue responsible for methyl methanethiosulfonate ( a membrane - permeable sulfhydryl modifier)-mediated enhancement of the binding of the ENT1 inhibitor nitrobenzylmercaptopurine riboside ( NBMPR ) in intact cells . - pubmed
We thus hypothesized that the inhibitory effects of the modifiers were due to the one or more of the six cysteine residues in the C - terminal half of ENT1 , particularly one or both of those in the fifth intracellular loop ( Cys414 and Cys416 ) . - pubmed
[ Protective effects of SUR2B / Kir6.1 potassium channel opener natakalim against RAVECs injuries induced by hypoxia ] . - pubmed
The cell survival rate was determined by MTT assay , con was measured using Griess Assay , RT - PCR was used to examine t he expression of intercellular adhesion molecule-1 ( ICAM-1 ) , vascular endothelial growth factor ( VEGF ) , endothelin-1 ( ET-1 ) mRNA in RAVEC . - pubmed
RESULTS : Natakalim could reverse hypoxia - induced changes in endothelial cell function , including increased endothelial cell survival rate and level of NO concentration , significantly inhibited the hypoxia - induced endothelial ICAM-1 , ET-1 , VEGF mRNA expression levels increased . - pubmed
CONCLUSION : Natakalim have protective effects on hypoxia - induced changes in endothelial cell function , increasing of permeation , excess expression of cell adhesion molecules . - pubmed
Attractor landscape analysis also suggested that combining nutlin-3 with inhibition of Wip1 would synergize to stimulate a sustained increase in p53 activity and promote p53 - mediated cell death . - pubmed
ZO-2 departure from the nucleus requires intact S257 , and stabilizing the beta - O - linked N - acetylglucosylation ( O - GlcNAc ) of S257 with O -( 2 - acetamido-2 - deoxy - d - glucopyranosylidene ) amino - N - phenylcarbamate , an inhibitor of O - GlcNAcase , triggers nuclear exportation and proteosomal degradation of ZO-2 . - pubmed
At the plasma membrane ZO-2 is not O - GlcNAc , and instead , as TJs mature , it becomes phosphorylated at S257 by protein kinase Czeta . - pubmed
Absorption of arginine - modified nanohydroxyapatite particles ( Arg - nHAP ) and DZ1 ( latent membrane protein 1 [ LMP1]-targeted ) reached nearly 100 % efficiency under in vitro conditions . - pubmed
Phorbol 12 - myristate 13 - acetate ( PMA ) , the most commonly used phorbol ester , binds to and activates protein kinase C ( PKC ) , causing a wide range of effects in cells and tissues . - pubmed
PMA induced the expression of c - fos gene transcription and protein expression , and led to activation of the MAPK pathways in NP cells . - pubmed
The c - fos promoter was suppressed completely in the presence of the MAPK inhibitor PD98059 , an inhibitor of the MEK / ERK kinase cascade , but not in the presence of SKF86002 , SB202190 , or SP600125 . - pubmed
Prior blockade of beta3 adrenoceptors ( SR59230A 10 mg / kg ip ) significantly reduced the amplitude of each temperature increase . - pubmed
Membrane stabilizing agents , which include the anticonvulsants , antiarrhythmics and probably corticosteroids , may act by blocking sodium channels on damaged neural membranes . - pubmed
Prescriptions for long - term management varied among districts , as follows : inhaled corticosteroids ( ICS ) , mean of 37.3 % ( 0 - 66.7 % ) ; ICS / long - acting beta - agonists ( LABA ) , 27.9 % ( 16.7 - 66.7 % ) ; and leukotriene receptor antagonist ( LTRA ) , 20.5 % ( 0 - 50.0 % ) CONCLUSION : These findings suggest that asthma treatment in emergency settings varies by facility and region . - pubmed
At the age of 4 mo , he was diagnosed as having CNDI , judging from fever with hypernatremia ( serum Na 153 mEq / L ) , diluted urine ( urine osmolarity 193 mOsm / kg ) , high antidiuretic hormone ( plasma antidiuretic hormone 53 pg / mL ) , and normal renal function ( serum creatinine 0.3 mg / dL ) . - pubmed
Pre - adolescents in " dry " communities ( with laws restricting alcohol use ) had much higher lifetime and past 30 - day HLP use . - pubmed
The data produced indicated that astroglial cells contain P450 - reductase which transforms paraquat into a pyridium free - radical . - pubmed
The effect of simvastatin on inflammatory cytokines in community - acquired pneumonia : a randomised , double - blind , placebo - controlled trial . - pubmed
The secondary end points were serum concentrations of inflammatory cytokines and partial pressure of arterial oxygen / fractional inspired oxygen ( PaO2 / FiO2 ) at 48 h after treatment administration . - pubmed
No significant differences in PaO2 / FiO2 ( p=0.37 ) , C reactive protein ( p=0.23 ) , tumour necrosis factor - alpha ( p=0.58 ) , interleukin 6 ( IL-6 ; p=0.64 ) , and IL-10 ( p=0.61 ) levels at 48 h of hospitalisation were found between simvastatin and placebo groups . - pubmed
In this work , we determined the reference intervals ( RIs ) for total cholesterol ( TC ) , high - density lipoprotein cholesterol ( HDL - c ) , non - high - density lipoprotein cholesterol ( nHDL - c ) , low - density lipoprotein cholesterol ( LDL - c ) and triglycerides ( TG ) in Brazilian healthy children and adolescents . - pubmed
For the LDL - cholesterol , the values corresponding to the percentiles above , aged 1 to 8 years and 9 to 12 years , were 132 mg / dL 139 mg / dL , respectively . - pubmed
Design , synthesis and docking studies of novel thienopyrimidine derivatives bearing chromone moiety as mTOR / PI3Kalpha inhibitors . - pubmed
Bisulfite sequencing evaluated DNA methylation of placenta - specific 8 ( PLAC8 ) . - pubmed
Methylation status of 17 CpG sites in PLAC8 negatively correlated with mRNA expression . - pubmed
Following the recommendations of the WHO , Cameroon changed its malaria treatment policy from monotherapy to artemisinin - based combination therapy ( ACT ) as the first - line treatment for uncomplicated malaria . - pubmed
The anti - malarials prescribed were Anti - malaria combination therapy ( ACT ) - 1216 ( 47.6 % ) , quinine 1044 ( 40.8 % ) or SP 296 ( 11.6 % ) . - pubmed
Although there is an overall increase in the prescription of ACT , less prescription of quinine and a noticeable restrain from prescription of SP to febrile cases , the old practice was still rampant . - pubmed
OBJECTIVE : To preliminarily develop a surfactant - free , polymeric solid dispersion ( PSD ) of paclitaxel suitable for oral administration . - pubmed
The absolute oral bioavailability ( AOB , 20 mg / kg ) of the three formulas [ HPMCAS / paclitaxel / WCB = 4/1/0 ( F1 ) , 8/1/0 ( F2 ) and 4/1/4 ( F3 ) , w / w / w ] were 11.8 , 13.6 and 25.6 % , respectively . - pubmed
The AOB of F3 is nearly seven times higher than that ( 3.8 % ) of paclitaxel material ( a control ) . - pubmed
The advantage of higher HPMCAS / paclitaxel ratio of F2 in a dissolution test was not reflected in the first in vivo test due to the relatively higher dose of polymer which could not be effectively dissolved under the limitation of intestinal environment . - pubmed
Further characterization of the effect of ethanol on voltage - gated Ca ( 2 + ) channel function in developing CA3 hippocampal pyramidal neurons . - pubmed
We previously reported that acute and repeated exposure to ethanol during the third trimester - equivalent inhibits long - term potentiation of GABAA receptor - dependent synaptic currents in CA3 pyramidal neurons through a mechanism that depends on retrograde release of brain - derived neurotrophic factor driven by activation of voltage - gated Ca ( 2 + ) channels ( Zucca and Valenzuela , 2010 ) . - pubmed
Here , we further investigated the acute effect of ethanol on the function of voltage - gated Ca ( 2 + ) channels in CA3 pyramidal neurons using Ca ( 2 + ) imaging techniques . - pubmed
To investigate the long - term consequences of ethanol on voltage - gated Ca ( 2 + ) channels , we used patch - clamp electrophysiological techniques to assess the function of L - type voltage - gated Ca ( 2 + ) channels during and following ten days of vapor ethanol exposure . - pubmed
Taken together with our previous findings , our results suggest that 3(rd ) trimester - equivalent ethanol exposure transiently inhibits L - type voltage - gated Ca ( 2 + ) channel function in CA3 pyramidal neurons and that compensatory mechanisms restore their function during ethanol withdrawal . - pubmed
Assessment of the performance of the continuous improvement programmes based on six measures comprising the post - caesarean rehabilitation score : duration infusion , early oral analgesia , time to removal of the urinary catheter , time to return to drinking , eating recovery time , use of carbetocin . - pubmed
Intact mitochondrial Ca2 + uniport is essential for agonist - induced activation of endothelial nitric oxide synthase ( eNOS ) . - pubmed
The knock - down of mitochondrial calcium uptake 1 ( MICU1 ) , a gatekeeper of the MCU , increased both mitochondrial Ca2 + sequestration and Ca2 + -induced NO signals . - pubmed
The positive correlation between mitochondrial Ca2 + elevation and NO production was independent of eNOS phosphorylation at serine1177 . - pubmed
Our findings emphasize that manipulating mitochondrial Ca2 + uptake may represent a novel strategy to control eNOS - mediated NO production . - pubmed
Corrigendum to " GENEDIA multi influenza Ag rapid test for detection and H1 , H3 , and H5 subtyping of influenza viruses " [ J. Clin . - pubmed
Several kinases and phosphatases have been shown to be crucial for ABA - dependent regulation of the ion transporters . - pubmed
To date , the Ca2 + -dependent regulation of Shaker channels by Ca2 + - dependent protein kinases ( CPKs ) is still elusive . - pubmed
Furthermore , Ca2 + -induced stomatal closure is impaired in two cpk33 mutant plants . - pubmed
Multicellular spheroids can be achieved within 24 h and further boosted by incorporating collagen fibrils in PDMS - HDA . - pubmed
For laboratory evaluation , cholestasis parameters ( bilirubin , gamma - GT ) , liver cell parameters ( ASAT , ALAT ) and liver synthesis parameters ( quick , albumin ) were determined . - pubmed
RESULTS : In chemical laboratory and histological evaluation , a distinction between acute and chronic cholestatic liver injury with identification of inflammation and regeneration could be demonstrated by an increase in cholestasis ( bilirubin : 1 - wk group , 156.83 + - 34.12 mumol / L , P = 0.004 ) and liver cell parameters ( ASAT : 2 - wk group , 2.1 + - 2.19 mumol / L.s , P = 0.03 ; ALAT : 2 - wk group , 1.03 + - 0.38 mumol / L.s , P = 0.03 ) after bile duct ligation ( BDL ) . - pubmed
The following anthroponetric parameters were determined : body mass index ( BMI ) , waist to hip ratio , blood lipid profile ( total cholesterol , triglycerides , high density lipoproteins ( HDL ) , and low density lipoproteins ( LDL ) ) . - pubmed
Leptin and insulin levels were measured using com- mercial ELISA kits & lt;<Leptin ELISA>> ; and & lt;<Insulin ELISA>> ; ( DRG International , Inc. , USA ) respectively Insulin resistance in- dex HOMA -JR ( Homeostasis Model Assessment of Insulin Resistance ) was calculated . - pubmed
Signficant direct correlations between leptin blood levels and BMI , HOMA - IR index , triglycerides , and cholesterol were documented The relationships between blood levels of leptin and the thickness of the gallbladder ( GB ) wall , the amount of cholesterol crystals in bile , and decreased bile release rate from GB which suggests effect of leptin on the structural and finctional changes in GB . - pubmed
To improve cellular uptake and transport of pure nanodrug in cells , here , a lipid covered saquinavir ( SQV ) pure drug NP ( Lipo@nanodrug ) was designed by modifying a pure SQV NP ( nanodrug ) with a phospholipid bilayer . - pubmed
Although the exact mechanism of the development of ovarian cysts is unknown , it is thought that increased androgen levels stimulate folicle development by increasing follicle stimulating hormone ( FSH ) levels . - pubmed
Laboratory test results were as follows : sodium : 126 mEq / L , potassium : 5.4 mEq / L , renin : 132 pg / mL , adrenocorticotropic hormone ( ACTH ): 207 pg / mL , cortisole : 7.8 mug / dL , basal 17OH progesterone : 21 ng / mL , androstenedione : 5.1 ng / mL , testosterone : 1188 ng / dL and dehydroepiandrosterone sulfate ( DHEAS)>1500 mug / dL. - pubmed
CONCLUSIONS : The findings in our patient suggest that a decline in adrenal androgens after glucocorticoid treatment resulted in an increase in gonadotropin levels and the giant cyst is developed by activation of gonadotropin cascade and increased gonadotropin receptors , instead of androgens . - pubmed
Our results also supported that more aerial nodal roots and fewer crown roots might be favored in temperate maize , and this root architecture might efficiently improve root - lodging resistance and the ability to take up deep water and nitrogen under dense planting . - pubmed
Cadmium disrupts signaling of the hypoxia - inducible ( HIF ) and transforming growth factor ( TGF - beta ) pathways in placental JEG-3 trophoblast cells via reactive oxygen species . - pubmed
The induction of cytosolic reactive oxygen species ( ROS ) , expression of metallothionein ( MT ) isoforms , HIF1alpha and downstream targets , and expression of TGFbeta pathway - associated genes and proteins were assessed . - pubmed
Additionally , the effect of pre - treatment with the antioxidant N - acetyl cysteine ( NAC ) on ROS generation and effects on HIF , MT and TGF - beta signaling pathways was examined . - pubmed
Cd and Cd - mixture treated cells displayed higher levels of ROSs with accompanying disruption of HIF and TGFbeta pathway signaling versus controls , with the Cd - mixture eliciting a greater effect . - pubmed
Conversely , pretreatment with NAC reduced Cd - induced ROS production and disruption of HIF , MT and TGFbeta pathway signaling . - pubmed
The plasmonic - photonic hybrid mode between the Au nanoparticles ( NP ) and the diatomite layer could supply nearly 10 times the increment of SERS signal ( MBA ) intensity compared to the common silica gel chromatography plate . - pubmed
The results indicated that the recombinant 140 amino acid protein , designated rMPN668 , displayed hydroperoxidase activity towards both organic ( tert - butyl hydroperoxide ) and inorganic ( hydrogen peroxide ) hydroperoxides in the presence of a reducing agent such as dithiothreitol . - pubmed
Additionally , homology modeling of MPN668 and a molecular dynamics simulation suggest that both Cys55 and Cys119 form part of the active site of the protein . - pubmed
Mutants in which Cys55 or Cys119 were replaced with a serine lack antioxidant activity , indicating that MPN668 is a Cys - based peroxidase , consistent with it representing a new member of the Ohr family . - pubmed
CYP2D6 genotype and endoxifen plasma concentration do not predict hot flash severity during tamoxifen therapy . - pubmed
Tamoxifen is a prodrug and requires bioactivation by CYP2D6 . - pubmed
There is paucity of prospectively collected data in terms of CYP2D6 genotype and measured tamoxifen , 4 - hydroxytamoxifen and endoxifen concentrations in relation to hot flash severity during tamoxifen therapy . - pubmed
Plasma concentrations of tamoxifen , 4 - hydroxytamoxifen , and endoxifen were measured using liquid chromatography - tandem mass spectrometry and genotyping was carried out for CYP2D6 . - pubmed
CONCLUSIONS : Hot flash severity during tamoxifen therapy can not be accounted for by CYP2D6 genotype or observed plasma concentration of tamoxifen , 4 - hydroxytamoxifen , or endoxifen . - pubmed
Inhibition of the sodium -- glucosecotransporter-2 ( SGLT2 ) can increase urinary glucose excretion and decrease plasma glucose levels in an insulin - independent manner . - pubmed
Advantages of ertugliflozin include reduction in glycated hemoglobin , weight loss and blood pressure lowering with a low risk of hypoglycemia . - pubmed
Synthesis , characterization , and SAR of arylated indenoquinoline - based cholinesterase and carbonic anhydrase inhibitors . - pubmed
Monophenyl ( 16 - 18 ) indenoquinolines significantly inhibited the AChE and BChE enzymes in ranges of IC50 37 - 57 nM and 84 - 93 nM , respectively , compared with their starting materials 15a - c and reference compounds ( galanthamine and tacrine ) . - pubmed
On the other hand , these novel arylated indenoquinoline - based derivatives were effective inhibitors of the BChE , hCA I and II , BChE and AChE enzymes with Ki values in the range of 37 + - 2.04 to 88640 + - 1990 nM for AChE , 120.94 + - 37.06 to 1150.95 + - 304.48 nM for hCA I , 267.58 + - 98.05 to 1568.16 + - 438.67 nM for hCA II , and 84 + - 3.86 to 144120 + - 2910 nM for BChE. - pubmed
As a result , monophenyl indenoquinolines 16 - 18 may have promising anti - Alzheimer drug potential and 3,8 - dibromoindenoquinoline amine ( 15f ) can be novel hCA I and hCA II enzyme inhibitors . - pubmed
25 - Hydroxyvitamin D ( 25 ( OH ) D ) , fasting plasma glucose and insulin were measured . - pubmed
The Food and Drug Administration ( FDA or we ) is classifying the high flow humidified oxygen delivery device into class II ( special controls ) . - pubmed
BACKGROUND : We conducted a single - arm phase II trial to evaluate the efficacy and adverse effects ( AEs ) of an anti - epidermal growth factor receptor monoclonal antibody , nimotuzumab , combined with cisplatin and 5 - fluorouracil ( PF ) as first - line treatment in recurrent metastatic nasopharyngeal carcinoma after radical radiotherapy . - pubmed
PGE2 and a selective EP3 agonist suppressed cytokine production and expression induced by polyinosine - polycytidylic acid stimulation , such as chemokine ligand 5 and chemokine motif ligand 10 . - pubmed
Here , we describe a novel liposome - based subunit vaccine formulation for tuberculosis ( TB ) based on phosphatidylserine encapsulating two prominent TB antigens , Ag85B , and ESAT-6 . - pubmed
An increasing initial osmotic pressure within the mineral medium decreases both glucose release and protease yield . - pubmed
This work presents the preparation of cross - linking Au nanoparticle ( NP ) monolayer membranes by the thiol exchange reaction and their enhanced mechanical properties . - pubmed
Moreover , the modulus of the cross - linking monolayer was measured by atomic force microscopy ( AFM ) and the result showed that the membrane with a longer dithiol molecule had a larger modulus , which might derive from the unbroken and intact structure of the cross - linking monolayer due to the selected appropriately lengthed dithiol . - pubmed
Data suggest that imbalance of metals , including copper , zinc , iron , and manganese , induces abnormalities in processing and degradation of prion proteins that are accompanied by self - propagation of PrPSc . - pubmed
Post - synthesis strategy to integrate porphyrinic metal - organic frameworks with CuS NPs for synergistic enhanced photo - therapy . - pubmed
Porphyrinic metal - organic frameworks with CuS NPs are obtained in aqueous solution via a simple post - synthesis strategy . - pubmed
EPR and magnetic susceptibility measurements helped us to assign the oxidation state of the manganese complexes in their native state . - pubmed
Preparation of theasinensin A and theasinensin B and exploration of their inhibitory mechanism on alpha - glucosidase . - pubmed
In the present study , TSA and TSB were chemically synthesized and purified , and their inhibitory effects on alpha - glucosidase and potential mechanisms were investigated . - pubmed
The results showed that TSA and TSB could inhibit the activity of alpha - glucosidase in a reversible and noncompetitive manner with IC50 values of 6.342 and 24.464 mug mL-1 , respectively , which were much lower than that of acarbose . - pubmed
The fluorescence and circular dichroism spectra revealed that TSA and TSB could alter the microenvironment and the secondary structure of alpha - glucosidase , thereby decreasing the alpha - glucosidase activity . - pubmed
Molecular docking results indicated that both TSA and TSB had a strong binding affinity to alpha - glucosidase by hydrophobic interactions and hydrogen bonds . - pubmed
Moreover , the stronger inhibition of TSA on alpha - glucosidase might be related to the closer binding site to the active site pocket of alpha - glucosidase . - pubmed
Oral prednisolone was administered on day 33 then fever subsided with dramatically clinical improvement ( decreased CRP and resolution of lung consolidation ) . - pubmed
RES treatment increased p - AMPK alpha / AMPK alpha and p - ULK1 S777 / ULK1 and the autophagy related proteins ( Beclin1 , LC3 II / I ) and its effects on TC and improvement in insulin resistence were quenched by the inhibitor of autophagy , 3 - MA . - pubmed
Together , these results suggest that the effect of RES in treatment of DN may involve AMPK alpha / mTOR - mediated autophagy . - pubmed
The dopamine transporter SPECT with 123I ioflupane showed abnormal scans in bilateral striatum . - pubmed
High levels of sPD-1 or sPDL1 were not correlated to PFS under bevacizumab while they were independent prognostic factors of PFS in the sunitinib group . - pubmed
In this study , response surface methodology ( RSM ) and box - behnken design ( BBD ) are used to investigate the stability of fiber in polymeric base suspensions , including carboxy methyl cellulose ( CMC ) , polyacrylamide ( PAM ) , and xanthan gum ( XG ) . - pubmed
Evaluation of a possible role of NaK - ATPase in transtubular sodium reabsorption is difficult , since ouabain doses that inhibit this enzyme system in vitro completely [ 1 , 11 ] , can not be applied in vivo . - pubmed
However , in this model , supramaximal doses of ouabain as used to block NaK - ATPase completely induced a potent vasoconstriction , which lowers the filtered load of sodium . - pubmed
In the following experiments we reinvestigated the relationship between inhibition of renal NaK - ATPase and the reduction of Na - reabsorption by ouabain under more appropriate conditions . - pubmed
TGF beta 1 mRNA , but not TGF beta 2 or TGF beta 3 mRNA , was also produced constitutively by all four cell lines . - pubmed
Quantitation by bioassay revealed baseline TGF beta secretion of approximately 1 ng/10(6 ) cells over 48 h. Stimulation of mastocytoma cells with phorbol ester increased the rate of release of TGF beta 1 , most markedly in the first 30 min after stimulation , without increasing TGF beta 1 mRNA . - pubmed
It is suggested that apo E secreted from peripheral tissues enhances the uptake of lipoproteins by themselves or by surrounding cells in the local environment which demand cholesterol and express LDL receptors . - pubmed
Post - marketing surveillance of quinolones 1988 - 1990 . - pubmed
It has been much easier to obtain original data on adverse drug reactions ( ADR ) of quinolones from the pharmaceutical industry than it was two years ago . - pubmed
This is to be welcomed and , as anticipated , the new data continue to suggest that the new 4 - quinolones have an ADR profile which is very similar to that of other antimicrobials . - pubmed
Visual disturbance is not a prominent feature , in contrast to the ADR profile of nalidixic acid . - pubmed
After VF , tissue concentrations of ATP , AMP , creatinine phosphate ( CP ) , and lactate were measured . - pubmed
After NE , but not E , tissue AMP concentrations were significantly increased , and CP concentrations were reduced compared with vehicle ( P less than 0.05 ) . - pubmed
Tissue levels of adenosine triphosphate ( a major tissue energy store ) , superoxide dismutase ( a major defense against free radicals ) , xanthine oxidase ( an enzymatic source of free radicals ) , and edema were measured . - pubmed
Photoaffinity labelling of dopamine D2 receptors by [ 3H ] azidomethylspiperone . - pubmed
We have characterized the dopamine D2 receptor photoaffinity probe , [ 3H ] azido - N - methylspiperone ( [ 3H ] AMS ) . - pubmed
However , no direct correlation between the increasing sensitivity to antigen and the decreasing reactivity to IL2 appears to exist . - pubmed
In TAM from all tumors studied the expression of PA was markedly greater than that of resident peritoneal macrophages . - pubmed
The fact that PA was similarly enhanced in peritoneal macrophages responding to a standard stimulation ( thioglycolate ) and in TAM suggests that the expression of PA is part of a more general cellular inflammatory response to injury . - pubmed
Basal serum luteinizing hormone ( LH ) concentration was increased by NAL during each trial in the luteal phase and LH pulse amplitude was proportionately increased by 158 % , 164 % and 350 % during the early luteal , mid luteal and early follicular phases , respectively . - pubmed
The apparent NAL induced increase ( 92 % ) in LH pulse amplitude during the late luteal phase was not significant . - pubmed
NAL only affected LH pulse frequency during the early follicular phase , when it was decreased . - pubmed
Mean serum follicle stimulating hormone ( FSH ) concentration was not affected by NAL . - pubmed
The results of this study indicate that endogenous opioid peptides ( EOPs ) may partially mediate the suppressive influence of estradiol-17 beta ( E2 ) on LH pulse amplitude and also the stimulatory effect of E2 on LH pulse frequency in the early follicular phase . - pubmed
The data may suggest that NAL enhances the amplitude of pulses of gonadotropin releasing hormone ( GnRH ) by counteracting E2 inhibitory effects on LH release at the level of the pituitary . - pubmed
This report describes the initial characterization of small muscle - associated connective tissue proteoglycans synthesized in embryonic chick leg in ovo and by embryonic leg muscle - associated secondary fibroblasts in vitro by CsCl equilibrium density gradient centrifugation . - pubmed
Formation of mixed disulfide adducts at cysteine-281 of the lactose repressor protein affects operator and inducer binding parameters . - pubmed
The lactose repressor protein has been modified with three sulfhydryl - specific reagents which form mixed disulfide adducts . - pubmed
Modification of the lac repressor with didanylcystine decreased operator binding only 4 - fold , and nonspecific DNA binding increased 3 - fold compared to unmodified repressor . - pubmed
Nuclear magnetic resonance studies of protein binding sites : the complex of staphylococcal nuclease with 3 ' , 5 '- thymidine diphosphate . - pubmed
the biosynthetic pathway ( 17 beta - HSD , delta 5 - 3 beta - HSD ) leading towards the androgens was enhanced in the abdominal skin . - pubmed
The lithium clearance technique has been proposed as a non - invasive method whereby fluid delivery from the pars recta and pars convoluta of proximal tubules can be measured as CLi and CIN [ 0.78 CLi / CIN + 0.22 ] , respectively [ 12 ] , CLi being the clearance of lithium and CIN that of inulin . - pubmed
Analysis of cyclosporin interactions with antibodies and cyclophilin using the BIAcore . - pubmed
The immunosuppressive cyclic undecapeptide cyclosporin A ( CS ) exists in various conformers in water . - pubmed
We conclude that some patients with PPS have in the serum high titers of IgM anti - PV antibodies , implying an ongoing antibody response to a viral antigen . - pubmed
We have previously identified six mutations in the G6Pase gene of glycogen storage disease type 1a patients and demonstrated that these mutations abolished or greatly reduced enzymatic activity of G6Pase , a hydrophobic protein of 357 amino acids . - pubmed
To further understand the roles and structural requirements of amino acids 83 , 222 , 295 , and those at the carboxyl terminus in G6Pase catalysis , we characterized mutant G6Pases generated by near - saturation mutagenesis of the aforementioned amino acids . - pubmed
Substitution of Arg-83 with amino acids of diverse structures including Lys , a conservative change , yielded mutant G6Pase with no enzymatic activity . - pubmed
All other substitutions of Arg-295 had no G6Pase activity , suggesting that the role of Arg-295 is to stabilize the protein either by salt bridge or hydrogen - bond formation . - pubmed
It is possible that Arg-83 is involved in stabilizing the enzyme ( His)-phosphate intermediate formed during G6Pase catalysis . - pubmed
Substitution of His-119 with amino acids of diverse structures also yielded mutant G6Pase with no activity , suggesting that His-119 is the phosphate acceptor in G6Pase catalysis . - pubmed
Familial high serum concentrations of the carboxyl - terminal propeptide of type I procollagen . - pubmed
We describe a family with an apparently autosomal - dominant trait that caused extremely high circulating concentrations of the carboxyl - terminal propeptide of type I procollagen ( PICP ) . - pubmed
Furthermore , their serum concentrations of the amino - terminal propeptide of type I procollagen ( PINP ) were normal . - pubmed
Amino acid analysis , mass determination ( 2,165 Da ) and N - terminal sequencing ( Ile - Ala - Xaa - Lys - Phe - Ile - Xaa - Xaa - Pro - Gly - Xaa - Ala - Lys - block ) demonstrated that staphylococcin 1580 is identical to epidermin , a lanthionine - containing antibiotic peptide ( lantibiotic ) . - pubmed
The data support a model in which the state of correlation of pre- and post - synaptic activity is converted by the voltage - dependent NMDA receptor channel into a graded postsynaptic Ca2 + signal . - pubmed
Another group of neurotransmitters , including 5 - hydroxytryptamine ( 5 - HT ) , thyrotropin - releasing hormone ( TRH ) and gamma - aminobutyric acid ( GABA ) was never expressed in situ in DRG neurons . - pubmed
Similar effects were observed when spermatozoa were incubated with phosphodiesterase inhibitors or cell - permeant cAMP analogs , suggesting that cAMP is involved in these two processes . - pubmed
The inhibition of these phenomena by cAMP - dependent protein kinase inhibitors , and the stimulation by protein phosphatase inhibitors , suggest that Ser / Thr protein phosphorylation plays an important role in the regulation of both sperm capacitation and protein - tyrosine phosphorylation pathways . - pubmed
However , observations that both calyculin A and okadaic acid stimulated sperm capacitation , whereas only calyculin A increased p105 and p81 phosphotyrosine content and sperm velocity , suggest that protein phosphatase PP1 is involved in the two latter phenomena while PP2A mediates sperm capacitation . - pubmed
These results suggest that divergent pathways might regulate tyrosine phosphorylation of p105 and p81 and sperm capacitation after cAMP - dependent phosphorylation of an intermediate protein . - pubmed
These findings were basically consistent with those in apoptosis induced by a monoclonal antibody against the surface antigen Fas . - pubmed
Expression patterns of brush - border antigen as well as molecular markers of kidney differentiation Wt1 , Lim1 , Hgf and c - met , c - ret , Shh , Wnt4 , Wnt7b , and Wnt11 were analyzed in explants by whole mount and tissue section in situ hybridization following 1 - 9 days in culture . - pubmed
Mycobacterial cell wall components were assessed by ELISA for their ability to bind the carbohydrate recognition domain of rMBP . - pubmed
The best ligand was mannosyl - lipoarabinomannan , followed by lipomannan , phosphatidylinositol mannoside , arabinosyl - lipoarabinomannan , and dimycolated trehalose ( cord factor ) . - pubmed
rMBP did not bind to partially purified lipid fractions containing glycopeptidolipids . - pubmed
Previous studies have shown that adrenalectomy results in a small increase in hippocampal alpha - bungarotoxin binding , whereas seven days of chronic treatment with high doses of corticosterone results in decreases in alpha - bungarotoxin binding in several brain regions . - pubmed
The studies reported here examined the effects of different doses of corticosterone on brain alpha - bungarotoxin binding . - pubmed
These results argue that corticosterone levels play an important role in modulating the level of the brain nicotinic receptors that bind alpha - bungarotoxin with high affinity . - pubmed
Complementation analysis with 15 reference single - site loci ( cistrons ) in the zeste - white region of the X chromosome revealed that 29 of 723 urethane - induced recessive lethals were located in the zeste - white region and all were restricted to a single locus . - pubmed
Positron emission tomography - based boron neutron capture therapy using boronophenylalanine for high - grade gliomas : part II . - pubmed
In the Born test 4 - chlorophenylethenecarboxamidoxime ( 21 ) was most active and inhibited the blood platelet aggregation induced by collagen with an IC50 = 3 microM. - pubmed
The THF extracts were derivatized with tert - butyldimethylsilyl ( t - BDMS ) for GC - MS analysis . - pubmed
A single crystal separated from the extract was used for an x - ray structure analysis , giving the monoclinic unit cell , space group P21 / c with ao = 5.543 A , bo = 8.845 A , co = 5.086 A , and beta = 91.9 degrees , consistent with beta - succinic acid , HOOC ( CH2 ) COOH . - pubmed
We determined the affinities of eight novel histamine H ( 3 )- receptor ligands ( ethers and carbamates ) for H ( 3 )- receptor binding sites and their agonistic / antagonistic effects in two functional H ( 3 )- receptor models . - pubmed
Only FUB 373 and 407 inhibited the evoked overflow by themselves ( intrinsic activities 0.3 and 0.4 ) ; these effects were counteracted by the H ( 3 )- receptor antagonist clobenpropit . - pubmed
In conclusion , the novel compounds are partial H ( 3 )- receptor agonists ( FUB 373 and 407 ) or H ( 3 )- receptor antagonists ; comparison with FUB 335 shows that the transition from antagonist to agonist is caused by a slight structural change . - pubmed
They had no effect on the cell influx , whereas the A3 antagonist , MRS-1220 , significantly inhibited cellular infiltration , suggesting mediation through A3 receptors . - pubmed
CONCLUSIONS : 5 '- AMP caused a rapid migration of eosinophils and macrophages into the airways only in sensitized guinea - pigs , and this was blocked by the A3 antagonist MRS-1220 . - pubmed
This adhI mutant expressed high levels of a novel alcohol dehydrogenase ( AdhI ) that uses ethanol , 1 - propanol and 2 - propanol as substrates . - pubmed
Second trimester maternal serum alpha - fetoprotein ( MS - AFP ) , human chorionic gonadotrophin ( hCG ) , unconjugated estiol ( uE3 ) , and inhibin - A ( INH - A ) levels were evaluated in pregnancies complicated by triploidy . - pubmed
However , there were the characteristic decreases in plasma atrial natriuretic peptide concentrations , and increases in plasma and urinary cortisol . - pubmed
Atrial and ventricular signals were recorded during sinus rhythm ( SR ) , atrial flutter , AF , and VT or VF . - pubmed
During VF atrial " sinus " signals had significantly ( P & lt ; 0.01 ) lower amplitudes ( 1.4 + /- 0.52 mV ) than during SR . - pubmed
Augmented expression of P - gp / multi - drug resistance gene by all - trans retinoic acid in monocytic leukemic cells . - pubmed
By liquid culture of leukemia cell lines and fresh AML cells with the addition of all - trans retinoic acid ( ATRA ) , modulation of P - gp / MDR1 and Egr1 was observed and the pattern of modulation was divided into four groups : ( 1 ) blastic AML type , in which distinct expression of P - gp / MDR1 and CD34 was not influenced by ATRA ; ( 2 ) t(8;21)AML type , in which P - gp / MDR1 expression was augmented by ATRA , while CD34 was kept high ; ( 3 ) AML M3 type , in which P - gp / MDR1 expression was reduced with granulocytic differentiation by ATRA ; ( 4 ) monocytic AML type , in which P - gp / MDR1 expression was augmented by ATRA , while CD34 expression decreased , and strong Egr1 expression was downregulated just prior to the augmentation of P - gp / MDR1 expression . - pubmed
WT1 expression was not influenced by the addition of ATRA in each group . - pubmed
However , P - gp / MDR1 expression augmented by ATRA , which was observed in monocytic AML , was recognized as a functional molecule of mature monocyte / macrophage , because CD34 expression decreased and CD13 expression increased by ATRA . - pubmed
Finally , expression of P - gp / MDR1 in monocytic leukemia , which was functionally confirmed by Rh123 efflux study , was thought to be closely related to the characteristic modulation of Egr1 expression by ATRA . - pubmed
Membrane - bound transhydrogenases are conformationally driven proton - pumps which couple an inward proton translocation to the reversible reduction of NADP+ by NADH ( forward reaction ) . - pubmed
The 2 - or 4 - hydroxypyrimidine - linked nitroreductase activity was inhibited by oxypurinol and ( + /-)- 8 -( 3 - methoxy-4 - phenylsulfinylphenyl ) pyrazolo [ 1,5 - a ]- 1,3,5 - triazine-4 ( 1H )- one ( BOF-4272 ) , inhibitors of xanthine oxidase , but not by menadione , chlorpromazine and isovanillin , inhibitors of aldehyde oxidase . - pubmed
When skin cytosol was applied to a DEAE - cellulose column , the fractions containing xanthine oxidase exhibited a marked 2 - hydroxypyrimidine - linked nitroreductase activity . - pubmed
Moreover , the xanthine oxidase fraction exhibited a significant nitroreductase activity in the presence of 2 - hydroxypyrimidine , 4 - hydroxypyrimidine or hypoxanthine , and these activities were inhibited by inhibitors of xanthine oxidase . - pubmed
These results indicated that reduction of 2 - nitrofluorene in the skin was mainly catalyzed by xanthine oxidase . - pubmed
[ ACTH and cortisone in the treatment of chronic lymphatic leukemia ] . - pubmed
LIPASE FORMATION BY MICRO - ORGANISMS GROWN ON HYDROCARBONS . - pubmed
ON THE ROLE OF FATTY ACID PEROXIDES IN ATHEROGENESIS . - pubmed
Effect of phosphodiesterase-5 inhibitor , sildenafil ( Viagra ) , in animal models of airways disease . - pubmed
Phosphodiesterase ( PDE )- 5 degrades guanosine 3 ' , 5'cyclic monophosphate ( cGMP ) and its inhibitor sildenafil citrate ( Viagra ) treats erectile dysfunction by smooth muscle relaxation through elevated cGMP . - pubmed
Twenty - four hours after ovalbumen exposure there was airway hyperreactivity ( AHR ) to inhaled histamine and significantly elevated macrophages , eosinophils , and nitric oxide ( NO ) metabolites in bronchoalveolar lavage fluid . - pubmed
Sildenafil treatment ( 1 mg / kg , intraperitoneally ) failed to affect the early and late responses but significantly reduced AHR , leukocyte influx , and elevated NO . - pubmed
LPS exposure ( 30 microg / ml ) caused AHR to histamine at 1 hour and macrophage , eosinophil , and neutrophil influx at 24 hours with raised NO . - pubmed
Sildenafil pretreatment inhibited LPS - induced AHR , leukocyte influx , and NO generation . - pubmed
The effectiveness of sildenafil was not dependent on endogenous NO because inhibition of NO synthase with Nomega - nitro - L - arginine methyl ester did not prevent its action . - pubmed
Inhibition of PDE5 by sildenafil was confirmed by elevated S - nitroso - N - acetylpenicillamine - induced cGMP generation in isolated lungs . - pubmed
A novel point mutation of the RET protooncogene involving the second intracellular tyrosine kinase domain in a family with medullary thyroid carcinoma . - pubmed
RET - activating mutations involve two important functional areas of the receptor : the cysteine - rich extracellular domain and the intracellular tyrosine kinase domain . - pubmed
Here , we describe a novel missense mutation of the RET protooncogene that substitutes arginine for proline at codon 912 of the intracellular tyrosine kinase domain in a family with medullary thyroid carcinoma . - pubmed
Morphological and cytological studies as well as determination of ABA content and analysis of the expression mode of a Lea gene , all point to its similarity to natural dormancy and germination of seeds . - pubmed
Hexokinase inhibitors such as glucosamine and N - acetyl - glucosamine did not exert any influence on the regulation - deregulation effects of sucrose . - pubmed
It is thus inferred that this sucrose - signaling pathway may be independent of hexokinase . - pubmed
This suggested the possibility that cSUT has an important role in this sucrose signal regulation system . - pubmed
SST , wogonin-7 - O - glucuronoside ( a major SST ingredient ) , and wogonin ( an intestinal metabolite of wogonin-7 - O - glucuronoside ) increased CD4 / CD8 ratio via a decrease of CD8 + T - cell counts with no effect on CD4 + T - cell counts . - pubmed
Flow cytometric analyses of viability , proliferation , and cell cycle revealed that wogonin suppressed CD8 + T - cell proliferation without inducing cell death . - pubmed
These findings suggest that SST may modulate the CD4 / CD8 ratio via the selective inhibition of CD8 + T - cell proliferation by the SST ingredient wogonin-7 - O - glucuronoside or its metabolite wogonin . - pubmed
A canted antiferromagnetic structure is observed below TN = 10 K , which remains stable down to 3 K , with an ordered magnetic moment of 2.44(7)mu(B ) for Co2 + cations . - pubmed
hominis cells were treated with MAb 1D5 or control MAb 5 , either with or without pretreatment with a pan - caspase inhibitor , zVAD.fmk , and/or a mitochondrial transition pore blocker , cyclosporine A ( CA ) . - pubmed
Flow cytometric examination of cell size , mitochondrial membrane potential ( delta psi(m ) ) , caspase activation and in situ DNA fragmentation showed that zVAD.fmk and CA , used independently or in combination , failed to inhibit MAb 1D5 - mediated PCD . - pubmed
The sections observed under light microscope to evaluate the results of hematoxylin and eosin ( HE ) staining , Masson staining or proliferative cell nuclear antigen(PCNA ) and alpha - smooth muscle actin ( alpha - SMA ) detected by immunohistochemistry . - pubmed
It contained putative copper - binding sites , a complement - like motif ( GCGWPRHM ) , a proteolytic activation site , and a conserved C - terminal region common to all known proPOs . - pubmed
The phage display technology offers an effective strategy for screening peptides which can interact with the anti - Gal antibody to block alpha - Gal antigen binding site . - pubmed
Two peptide libraries , linear 7 peptide library and C7C library , were panned on the anti - B monoclonal antibody which has the characteristic of binding to the alpha - Gal antigenic epitope . - pubmed
And new quantitative structure - activity relationships ( QSARs ) focused on the binding affinities of aryl hydrocarbon receptor ( AhR ) , aryl hydrocarbon hydroxylase ( AHH ) and 7 - ethoxyresorufin O - deethylase ( EROD ) induction potencies of PCDFs were developed . - pubmed
9,10 - Bis ( 4 - hexyloxyphenyl )- 2,7 - diiodophenanthrene was selectively obtained by a one - pot reaction of 10,10 - bis -( 4 - hexyloxy - phenyl )- 2,7 - diiodo-10H - phenanthren-9 - one with 1 equiv of triethylsilane in trifluoroacetic acid . - pubmed
INTRODUCTION : Cytochrome P450 1A2 ( CYP 1A2 ) is responsible for more than 90 % of caffeine clearance . - pubmed
Multivariate analysis revealed no evidence of any interaction effects of caffeine with CYP1A2 ( P=0.956 ) . - pubmed
Differentiation conditions caused a three- to fourfold elevation in cAMP level as well as cAMP - dependent protein kinase activity . - pubmed
Resistance against pro - oxidants from either stress or cAMP may be associated with upregulation of the expression of three major antioxidant genes , peroxidoxin 1 , trypanothione reductase , and superoxide dismutase A. - pubmed
In contrast , downregulation of intracellular cAMP by overexpression of cAMP phosphodiesterase A resulted in a decrease in resistance against oxidative damage and reduced infectivity toward activated macrophages . - pubmed
Superoxide dismutases ( SODs ) are a family of antioxidant enzymes that catalyse the degradation of toxic superoxide radicals in obligate and facultative aerobic organisms . - pubmed
All the genes identified ( sod1 to sod17 ) have been cloned and sequenced , and shown to encode potentially functional dimeric iron - containing SOD isozymes . - pubmed
cohnii cell lysate whereas MnSOD and Cu / ZnSOD were not detected . - pubmed
The nature of the resulting nanoparticles decorated multiwalled carbon nanotubes are characterized by scanning electron microscopy ( SEM ) and transmission electron microscopic ( TEM ) analysis . - pubmed
After debridement , the wounds were covered with rbFGF / ACS and then bound up with sterile gauze in the rbFGF / ACS group ( n = 30 ) , or bound up with petrolatum sterile gauze in the placebo group ( n = 28 ) . - pubmed
On serological examination , GLU , PL , K and ALT were increased significantly in 5 % females , and GLU was increased significantly and CRN was decreased significantly in the female 1.25 % group . - pubmed
It was not significantly associated with blood pressure , hypertension , serum total or high density lipoprotein cholesterol , body mass , diabetes , gout , osteoporosis , cardiovascular disease , smoking , caffeine use , or taking calcium channel blockers or anticholesterol medications . - pubmed
Some drugs ( antihistamines , antianxiety drugs , antidepressants , oral steroids ) are associated with greater risk , while angiotensin - converting enzyme inhibitors may be associated with lower risk . - pubmed
Seminal analysis of the baseline prior to incubation of samples with different concentrations of Alpha lipoic acids ( 0.00625 , 0.0125 , 0.025 , 0.05 , 0.1 mmol / ml ) was performed , and post - seminal analysis was conducted after a one - hour incubation . - pubmed
The cellular response to dsRNA or its synthetic analog polyinosinic - polycytidylic acid ( poly I : C ) results in IRF-3- , IRF-7- and NF - kB - mediated activation of type 1 IFNs and pro - inflammatory cytokines critical for innate antiviral immune responses . - pubmed
Ectopic expression and chronic activity of FGFR1 in hepatocytes accelerates diethylnitrosamine ( DEN)-initiated hepatocarcinogenesis by driving unrestrained cell proliferation and tumor angiogenesis . - pubmed
Hepatocyte FGFR4 mediates liver 's role in systemic cholesterol / bile acid and lipid metabolism and affects proper hepatolobular restoration after damage without effect on cell proliferation . - pubmed
Cast plates were fabricated from three types of gold alloys ( Type IV , Type II and low - gold ) and a Co - Cr alloy . - pubmed
In the specimens welded heterogeneously to Co - Cr , Type IV was the greatest , followed by low - gold and Type II . - pubmed
There was no statistical difference ( P<0.05 ) in fracture load between Type II control and that welded to Co - Cr . - pubmed
Higher elongations were obtained for Type II in all conditions , whereas the lowest elongation occurred for low - gold welded to Co - Cr . - pubmed
This study indicated that , of the three gold alloys tested , the Type IV gold alloy was the most suitable alloy for laser - welding to Co - Cr . - pubmed
Considering liver tumor oncologic procedures , the aim of this study was to evaluate the accuracy of liver iodine concentration ( IC ) estimated from CCT images under such challenging conditions . - pubmed
The accuracy of estimations using CCT and computed tomographic scanners was evaluated ; an elliptical cylinder water phantom with iodine solution inserts and seven patient data sets with transcatheter arterial chemoembolization were used . - pubmed
The deduced amino acid sequence showed to be identical with that of Bm - M55 , and the recombinant protein was about Mr 55000 . - pubmed
We also determined biological effects in tumors treated with the antibody alone or in combination with bevacizumab / avastin ( an anti - vascular endothelial growth factor antibody ) and/or gemcitabine ( a chemotherapeutic agent ) . - pubmed
When HDGF - H3 was combined with either bevacizumab or gemcitabine , we observed enhanced tumor growth inhibition , particularly when the three agents were used together . - pubmed
HDGF - H3 - treated tumors exhibited significant reduction of microvessel density with a pattern distinctive from the microvessel reduction pattern observed in bevacizumab - treated tumors . - pubmed
HDGF - H3 - treated but not bevacizumab - treated tumors also showed a significant increase of apoptosis . - pubmed
RESULTS : At 2 years , systolic and diastolic blood pressure ( SBP / DBP ) had decreased by 5.9/2.8 mm Hg ( 4.7/3.6 % ) in the added - potassium - and - calcium group and by 5.8/1.0 mm Hg ( 4.8/1.4 % ) in the salt - restricted group ; the values rose in the control group by 1.3/2.3 mm Hg ( 1.1/1.8 % ) . - pubmed
Enhancement of chemical derivatization of steroids by gas chromatography / mass spectrometry ( GC / MS ) . - pubmed
CONCLUSIONS : Most patients , who were regularly followed up in CKD clinics , were acceptably adherent to the prescribed dietary protein intake restrictions ; the LPD and MPD did not lead to protein wasting ; and the LPD did not decrease the risk of death or dialysis therapy initiation compared with the MPD . - pubmed
This effect requires a metabotropic glutamate receptor - mediated rise in Ca ( 2 + ) in the astrocyte processes surrounding the neuron and the release of the gliotransmitter ATP , which acts on postsynaptic purinergic receptors . - pubmed
All the treatments significantly reduced lactate dehydrogenase LDH efflux after reoxygenation ( -54.1 % for aspirin , -51.3 % for VOO and -72.9 % for aspirin plus VOO ) . - pubmed
The activity of the inducible isoform ( iNOS ) was inhibited by the three types of treatment ( -31.8 % for ASA , -29.1 % for VOO and -56.0 % for ASA plus VOO ) . - pubmed
The antibody , PIPA , protecting against small lethal challenges only is in the fraction A(2 ) containing mainly 11 S globulin . - pubmed
The PTs were incubated in a 50 % mixture of Ham 's F12 : Dulbecco 's medium containing only penicillin and dextran as supplements . - pubmed
CHRNB4 , which encodes the nAChR beta4 subunit , plays a major role in the molecular mechanisms that govern nicotine withdrawal . - pubmed
In addition , dietary astaxanthin increased concentrations of IgG and IgM , and B cell population . - pubmed
AIM : Oxidative stress is caused by imbalance between the productions of reactive oxygen species ( ROS ) and antioxidant defense mechanisms . - pubmed
Palm oil antioxidants such as tocotrienol rich fraction ( TRF ) is known to have neuroprotective effects on neurones by acting against free radical induced neuronal cell death . - pubmed
PURPOSE : The use of the high - dose corticotrophin stimulation test ( HDCST ) as a guide to use low - dose steroid therapy in septic shock is controversial . - pubmed
The adrenocotropin hormone ( ACTH ) constitutes the immediate stimuli to produce cortisol . - pubmed
However , we are not aware of any study investigating the effects of minocycline on caspase - independent programmed cell death ( PCD ) pathways . - pubmed
Minocycline exhibited neuroprotection and inhibition of the toxin - induced caspase-3 - like activity , DNA fragmentation , and chromatin condensation , hallmarks of apoptosis . - pubmed
Moreover , we revealed that 6 - OHDA also activated caspase - independent PCDs ( such as paraptosis ) , which required de novo protein synthesis . - pubmed
Additionally , by separately monitoring caspase - dependent and caspase - independent pathways , we showed that inhibition of apoptosis only partially explained the protective effect of minocycline . - pubmed
In conclusion , we report for the first time evidence indicating that minocycline may inhibit PCD pathways that are additional to conventional apoptosis . - pubmed
CONCLUSIONS : Our results show that claudin-6 protein is significantly down - regulated in breast invasive ductal carcinomas and is an important correlate with lymphatic metastasis , but claudin-6 down - regulation was not correlated with upregulation of the methylation associated proteins ( MeCP2 , DNMT1 ) or histone modification associated proteins ( HDAC1 , H3Ac , H4Ac ) . - pubmed
Treatment with vildagliptin showed increase in the number of insulin - positive beta - cells in islets and improved the expressions of genes and proteins are responsible for insulin secretions . - pubmed
Direct treatment with the three nitroderivatives decreased ROS generation compared to the control and NO - HTy at 20muM also increased glutathione peroxidase ( GPx ) activity ( p<0.001 ) . - pubmed
Pretreatment with the three nitroderivatives at 5 - 20 muM counteracted t - BOOH cell damage by decreasing ROS generation ( p<0.001 ) and malondialdehyde ( MDA ) levels ( p<0.001 ) , increasing reduced glutathione ( p<0.001 ) and disminishing GPx ( p<0.05 ) activity . - pubmed
NO - HTy , NO - HTy - A and NO - HTy - E decreased glutathione reductase activity ( p<0.05 ) . - pubmed
Our laboratory has previously demonstrated that reactive oxygen species , mediated by p22 ( phox )- based Nox oxidases , are enhanced in VHL - deficient cells and have a role in the activation of Akt on S473 , a site phosphorylated by the mTORC2 complex . - pubmed
Channel - mediated astrocytic glutamate release via Bestrophin-1 targets synaptic NMDARs . - pubmed
Our recent study demonstrated that astrocytes release glutamate upon GPCR activation via Ca2 + activated anion channel , Bestrophin-1 ( Best1 ) . - pubmed
The target of Best1 - mediated astrocytic glutamate has been shown to be the neuronal NMDA receptors ( NMDAR ) . - pubmed
FINDINGS : We recorded spontaneous miniature excitatory postsynaptic currents ( mEPSCs ) from CA1 pyramidal cells to test whether Best1 - mediated astrocytic glutamate targets synaptic NMDAR . - pubmed
An agonist of protease activated receptor 1 ( PAR1 ) was used to induce astrocytic Ca ( 2 + ) increase and glutamate release . - pubmed
Firstly , we found that activation of PAR1 and subsequent release of glutamate from astrocyte does not alone increase the frequency of mEPSCs . - pubmed
Astrocytic Best1 - mediated glutamate release by PAR1 activation did not affect decay kinetics , frequency , and amplitude of fast rising mEPSC . - pubmed
CONCLUSIONS : Our results indicate that astrocytic glutamate via Best1 channel targets and activates synaptic NMDARs . - pubmed
As a consequence , the serum cortisol half - life ( T1/2 ) is prolonged , ACTH is suppressed , and serum cortisol concentration is normal . - pubmed
We report three siblings - two female and one male - with the syndrome of apparent mineralocorticoid excess who presented with hypertension , hypokalemia , low renin , and low aldosterone levels . - pubmed
The addition of spironolactone resulted in a decrease in blood pressure , rise in serum potassium and a gradual increase in plasma renin activity in all three . - pubmed
Urinary steroid profile and molecular analysis confirmed the diagnosis of non - classic congenital adrenal hyperplasia ( NCCAH ) due to 21 - hydroxylase deficiency . - pubmed
Pretreated sample on enzymatic hydrolysis at 50 C for 20 h with cocktail of cellulase , xylanase and laccase produced by locally isolated consortia released 668.9 mg / g of total sugar and 265.06 mg / g of total polyphenols . - pubmed
Rab proteins are a large family of GTP - binding proteins that regulate cellular membrane traffic and organelle identity . - pubmed
Dephosphorylation of the E2P phosphoenzyme intermediate of the sarcoplasmic reticulum Ca ( 2 +)- ATPase was studied using density functional theory . - pubmed
The optimized structure of the transition state reveals considerable bond breakage between phosphorus and the Asp351 oxygen ( distance 2.19 A ) and little bond formation to the attacking water oxygen ( distance 2.26 A ) . - pubmed
These actions of clenbuterol were prevented by pre - treatment with the non - selective beta - adrenoceptor antagonist propranolol , the beta2 - adrenoceptor antagonist ICI-118,551 , but not the beta1 - adrenoceptor antagonist metoprolol , suggesting that the effects of clenbuterol on IkappaBalpha expression and NFkappaB activity are mediated specifically by the beta2 - adrenoceptor . - pubmed
In addition , the actions of clenbuterol were mimicked by systemic administration of another highly selective long - acting beta2 - adrenoceptor agonist formoterol . - pubmed
As neurodegenerative diseases are associated with inflammation we determined if clenbuterol could suppress NFkappaB activation that occurs in response to an inflammatory stimulus . - pubmed
In tandem , clenbuterol blocked expression of the NFkappaB - inducible genes TNF - alpha and ICAM-1 following LPS administration . - pubmed
Our finding that clenbuterol and formoterol inhibit NFkappaB activity in the CNS further supports the idea that beta2 - adrenoceptors may be an attractive target for treating neuroinflammation and combating inflammation - related neurodegeneration . - pubmed
These interactions are absent in a mutant strain with genomically encoded RNase E that lacks the C - terminal half , supporting the role of the carboxy - end domain as the scaffold for the degradosome . - pubmed
The data support a model for the RNA degradosome ( RNAD ) , in which the RNase E carboxy - end is proximal to PNPase , more distant to Enolase , and more than 10 nm from RhlB helicase . - pubmed
Replacement of the catalytic nucleophile aspartyl residue of dextran glucosidase by cysteine sulfinate enhances transglycosylation activity . - pubmed
This enzyme has an Asp-194 catalytic nucleophile and two catalytically unrelated Cys residues , Cys-129 and Cys-532 . - pubmed
The nucleophile mutant of 2CS was generated by substitution of Asp-194 with Cys ( D194C-2CS ) . - pubmed
As a member of imitation switch ( ISWI ) family in ATP - dependent chromatin remodeling factors , RSF complex consists of SNF2h ATPase and Rsf-1 . - pubmed
A snapshot of ubiquitin chain elongation : lysine 48 - tetra - ubiquitin slows down ubiquitination . - pubmed
Using " elongation intermediates " containing beta - catenin linked with Ub chains of defined length , it was observed that a Lys-48 - Ub chain of a length greater than four , but not its Lys-63 linkage counterparts , slowed the rate of additional Ub conjugation . - pubmed
Given that Lys-48 - tetra - Ub is packed into compact conformations due to extensive intrachain interactions between Ub subunits , this topology may limit the accessibility of SCF(betaTrCP)/Cdc34 to the distal Ub Lys-48 and result in slowed elongation . - pubmed
Glucose is transported across the cell membrane by specific saturable transport system , which includes two types of glucose transporters : 1 ) sodium dependent glucose transporters ( SGLTs ) which transport glucose against its concentration gradient and 2 ) sodium independent glucose transporters ( GLUTs ) , which transport glucose by facilitative diffusion in its concentration gradient . - pubmed
The more glycosylated GLUT1 is produced in brain microvasculature and ensures glucose transport across the blood brain barrier ( BBB ) . - pubmed
Glucose transported to astrocytes by GLUT1 is metabolized to lactate serving to neurons as energy source . - pubmed
GLUT2 is present in hypothalamic neurons and serves as a glucose sensor in regulation of food intake . - pubmed
GLUT3 is the most abundant glucose transporter in the brain having five times higher transport capacity than GLUT1 . - pubmed
GLUT5 is predominantly fructose transporter . - pubmed
In brain , GLUT5 is the only hexose transporter in microglia , whose regulation is not yet clear . - pubmed
GLUT4 and GLUT8 are insulin - regulated glucose transporters in neuronal cell bodies in the cortex and cerebellum , but mainly in the hippocampus and amygdala , where they maintain hippocampus - dependent cognitive functions . - pubmed
Insulin translocates GLUT4 from cytosol to plasma membrane to transport glucose into cells , and GLUT8 from cytosol to rough endoplasmic reticulum to recover redundant glucose to cytosol after protein glycosylation . - pubmed
For both SHP and CHP , the magnitude of hydraulic pressure that induced the greatest release of ATP during FSS was 15 mmHg . - pubmed
Decreasing the magnitude of pressure during FSS to atmospheric pressure reduced ATP release to that of basal ATP release from static cells and inhibited actin reorganization into stress fibers that normally occurred during FSS with 15 mmHg of pressure . - pubmed
Our study found that acetylcholine ( ACh ) was a key transmitter that derepressed the phenotype induced by LH . - pubmed
A phase II study of erlotinib monotherapy in pre - treated non - small cell lung cancer without EGFR gene mutation who have never / light smoking history : re - evaluation of EGFR gene status ( NEJ006 / TCOG0903 ) . - pubmed
CONCLUSIONS : Re - examination of EGFR gene status using different detecting method or different sample should be considered to grasp a chance of erlotinib treatment after first line treatment . - pubmed
In confirmed EGFR wild NSCLC , negative HGF staining could be a biomarker for longer PFS by erlotonib treatment . - pubmed
AIM : To evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems ( CS ) and hydroxyapatite ( HA - CS ) . - pubmed
After 24 h elution , cell viability was 10 + - 1.8 % , 49.5 + - 4.2 % and 61 + - 7.4 % , for MTA , and HA - CS , respectively . - pubmed
However , CS and HA - CS were significantly less toxic than the control material MTA ( P & lt ; 0.05 ) . - pubmed
Histopathological examination indicated similar inflammatory reaction , vascular congestion and connective tissue integrity associated with CS , HA - CS and MTA at each observation period ( P & gt ; 0.05 ) . - pubmed
The only significant difference was found for capsule thickness , that is thicker capsule was associated with HA - CS compared to MTA at 60 days ( P = 0.0039 ) . - pubmed
HA - CS induced moderately thick capsules ( median score 3 , score range 2 - 3 ) , whereas MTA resulted in thin capsule formation ( median score 2 , score range 1 - 3 ) . - pubmed
This treatment did not modify the SERCA1a affinity for Ca ( 2 + ) but increased the Ca ( 2 +)-dependent ATPase activity of SR membranes indicating that the S - acylation of SLN or of other proteins is required for this effect on SERCA1a . - pubmed
An alignment of 67 SLN sequences from the protein databases shows that 19 of them contain a cysteine and the rest a phenylalanine at position 9 . - pubmed
SITA severely impaired serum DPP-4 activity , but had no effect on glycaemia . - pubmed
In addition , SITA reduced resting tension of isolated single cardiomyocytes and intensified phosphorylation of the sarcomeric protein titin . - pubmed
SITA also increased LV concentrations of cGMP and increased activity of protein kinase G ( PKG ) . - pubmed
These effects seem at least partially mediated by stimulatory effects on the myocardial cGMP - PKG pathway and , hence , on the phosphorylation status of titin and the hereto coupled cardiomyocyte stiffness modulus . - pubmed
A sensitive strategy for the fluorescence detection of DNA methyltransferase activity based on the graphene oxide platform and T7 exonuclease - assisted cyclic signal amplification . - pubmed
In this work , a simple fluorescence strategy based on the graphene oxide ( GO ) platform and T7 exonuclease ( T7 Exo)-assisted cyclic signal amplification is developed for the fast and sensitive detection of DNA methyltransferase ( MTase ) activity and inhibition . - pubmed
The released ssDNA subsequently hybridized with a FAM - labeled signal probe ( DP ) to form a duplex with a blunt 5'-terminal of DP and a 4 - mer overhang at the 5'-end of the released ssDNA . - pubmed
Aquaporins Contribute to ABA - Triggered Stomatal Closure through OST1 - Mediated Phosphorylation . - pubmed
ABA induced a 2 - fold increase in osmotic water permeability ( Pf ) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells , which were both abrogated in pip2;1 plants . - pubmed
Open stomata 1 ( OST1)/Snf1 - related protein kinase 2.6 ( SnRK2.6 ) , a protein kinase involved in guard cell ABA signaling , was able to phosphorylate a cytosolic PIP2;1 peptide at Ser-121 . - pubmed
Upon expression in pip2;1 plants , a phosphomimetic form ( Ser121Asp ) but not a phosphodeficient form ( Ser121Ala ) of PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA - dependent activation and was able to restore ABA - dependent stomatal closure in pip2;1 . - pubmed
This work supports a model whereby ABA - triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide , through OST1 - dependent phosphorylation of PIP2;1 at Ser-121 . - pubmed
Insulin secretion and sensitivity were measured by the gold - standard Botnia clamp , which is a combination of a 1 - h intravenous glucose tolerance test ( IVGTT ) followed by 3 - h hyperinsulinemic euglycemic clamp . - pubmed
Fasting glucose and insulin were 7.3 + - 2.4 mmol / L and 10.6 + - 6.3 mU / L , respectively . - pubmed
Two epimeric metabolites of Larotaxel were synthesized in eight steps from 10 - DAB III as a commercial material and their structures were characterized using NMR and MS spectral data . - pubmed
Acetolactate synthase is a mitochondrial enzyme that catalyses the conversion of two pyruvate molecules to an acetolactate molecule with release of carbon dioxide . - pubmed
RESULTS : In Germany , mean levels of C16:1n-7 t , a marker for dairy and meat intake , decreased , as did mean levels of industrially produced ( IP)-TFA , as did the percentage of individuals with IP - TFA & gt ; 1.04 % . - pubmed
The HR for all - cause mortality of CACS & gt;=400 , in those with lower exercise capacity , is estimated to be about three times of that in those with higher exercise capacity ( HR 3.328 in & lt;10 METs vs. 1.108 in & gt;=10 METs , p for interaction = 0.024 ) after adjustment for age , gender , fasting glucose , creatinine , alanine transaminase and albumin . - pubmed
The symmetry of the room - temperature ( RT ) structure of title compounds La2 - xSrxCoTiO6 - delta changes with x , from P21 / n ( 0 & lt;= x & lt;= 0.2 ) to Pnma ( 0.3 & lt;= x & lt;= 0.5 ) and to R3c ( 0.6 & lt;= x & lt;= 1 ) . - pubmed
Therefore , this review focuses on the role that the interaction between vitamin D , VDR function , and gut microbiome might have on autoimmune diseases in the context of the hygiene hypothesis . - pubmed
The biologically active form of vitamin D , 1,25 ( OH ) 2D3 , serves as the primary ligand for VDRs , which have been shown to play a fundamental role in reducing autoimmune disease symptoms . - pubmed
Remarkably , evidence shows that hormonal compounds and byproducts of the microbiota such as secondary bile acids might also activate VDR . - pubmed
We have gained insights on how the VDR functions affects inflammation , autophagy , and microbiota composition that could lead to the development of pathogenesis of autoimmune diseases , while confirming the role vitamin D and VDRs have in the context of hygiene hypothesis . - pubmed
Although they have similar catalytic triads and oxyanion holes , esterases are hydrolases and homoserine transacetylases ( HTAs ) are transferases . - pubmed
Est22 could efficiently hydrolyze esters with acyl lengths of up to six carbon atoms but had no transacetylase activity , which is different from HTAs and HTA - like acetyl esterases . - pubmed
We solved the structures of Est22 and its L374D mutant and modeled the structure of the L374D mutant with p - nitrophenyl butyrate . - pubmed
Esterases catalyze the hydrolysis of simple esters and are widely used in the pharmaceutical and agrochemical industries , while HTAs catalyze the transfer of an acetyl group from acetyl - coenzyme A ( CoA ) to homoserine and are essential for microbial growth . - pubmed
The atypical vesicular glutamate transporter type 3 ( VGLUT3 ) is expressed by subpopulations of neurons using acetylcholine , GABA , or serotonin as neurotransmitters . - pubmed
SIGNIFICANCE STATEMENT VGLUT3 is an atypical member of the vesicular glutamate transporter family . - pubmed
Overall , BA contents decreased with NaCl level ( 2785.1mgkg-1 , 1148.1mgkg-1 and 307.7mgkg-1 ) and increased with ageing time ( 366.2mgkg-1 , 1711.8mgkg-1 and 2959.2mgkg-1 in the final product ( T0 ) , and after 1 ( T1 ) and 3 ( T3 ) months of ageing , respectively ) . - pubmed
The expression levels of osteoblast growth and differentiation factors ( runt - related transcription factor 2 , osteogenic protein-1 , and alkaline phosphatase ) , apoptosis - related genes ( transformation - related protein 53 , ataxia telangiectasia mutated protein , caspase-3 , and caspase-8 ) , and Akt1 and phospho - Akt ( Thr308 ) were then assessed via alkaline phosphatase staining , acridine orange - propidium iodide staining , annexin V / PI apoptosis assay , real - time RT - PCR , and Western blot analyses . - pubmed
Moreover , glucocorticoid treatment yielded increased transformation - related protein 53 , ataxia telangiectasia mutated protein , caspase-3 , and caspase-8 expression and decreased Akt1 and phospho - Akt levels , indicating glucocorticoid - induced apoptosis . - pubmed
Meanwhile , beta - ecdysone inhibited glucocorticoid function , preserving the expression of Akt1 and phospho - Akt and reducing the expression of transformation - related protein 53 , ataxia telangiectasia mutated protein , caspase-3 , and caspase-8 . - pubmed
Proprotein Convertase Subtilisin / Kexin Type 9 Inhibitor Therapy - Breakthrough in Low - Density Lipoprotein Cholesterol Lowering , Breakdown in Value . - pubmed
Here , we demonstrate the stability and dynamics of a tile - based 6 - helix DNA nanotube ( DNT ) embedded in POPC lipid bilayer using the analysis of 0.2 mus long equilibrium MD simulation trajectories . - pubmed
We observe that the head groups of the lipid molecules close to the lumen cooperatively tilt towards the hydrophilic sugar - phosphate backbone of DNA and form a toroidal structure around the patch of DNT protruding in the membrane . - pubmed
In this study , the effect on pore volume and compressive strength was investigated using nano - sized blast furnace slag ( BFS ) , fly ash ( FA ) , and desulfurized gypsum ( DG ) . - pubmed
In the chemical compositions of BFS and FA , the sum of the four ingredients , CaO , SiO2 , A12O3 , and MgO were shown to account for 97 % and 87 % , respectively . - pubmed
The mean CA-125 level was 80.04 U / mL at baseline and significantly decreased to 33.11 U / mL after taking DNG for 24 weeks and lasted until 72 weeks ( P for trend = .0288 ) . - pubmed
Then the Congo red ( CR ) removal efficiency by DAB was optimized based on the flocculation conditions , including wastewater initial pH , flocculant dosage and initial dye concentration . - pubmed
A Phase IV Efficacy Study of Formeta Plus Carboplatin as First - Line Treatment of Advanced Non - Squamous , Non - SmallCell Lung Cancer in Iran : An Affordable Price with Clinical Benefit Background : This study performed to assess the efficacy and safety of Formeta ( generic form of Pemetrexed ) plusCarboplatin as first - line chemotherapy in advanced stage , non - squamous , non small cell lung cancer ( NSCLC ) in Iran . - pubmed
Methods : This was a post marketing single - arm phase IV efficacy study of Formeta ( manufactured by Oncomed . - pubmed
R406 elicits anti - Warburg effect via Syk - dependent and -independent mechanisms to trigger apoptosis in glioma stem cells . - pubmed
Utilizing an anti - cancer compound library , we identified R406 , the active metabolite of a FDA - approved Syk inhibitor for immune thrombocytopenia ( ITP ) , with remarkable cytotoxicity against GSCs but not normal neural stem cells . - pubmed
Mechanistically , the anti - GSC effect of R406 was due to the disruption of Syk / PI3 K signaling in Syk - positive GSCs and PI3K / Akt pathway in Syk - negative GSCs respectively . - pubmed
Overall , these findings not only identify R406 as a promising GSC - targeting agent but also reveal the important role of Syk and PI3 K pathways in the regulation of energy metabolism in GSCs . - pubmed
RESULTS : Post - warming trypan blue staining showed the survival of most cells , although membrane integrity immediately after warming ( 66.00 % + - 4.73 ) or after 2 h IVC ( 59.67 % + - 4.18 ) was significantly lower than controls ( C0h 89.67 % + - 1.45 ) . - pubmed
Synthesis , Structural Studies , and alpha - Glucosidase Inhibitory , Antidiabetic , and Antioxidant Activities of 2,3 - Dihydroquinazolin-4 ( 1H )- ones Derived from Pyrazol-4 - carbaldehyde and Anilines . - pubmed
A series of new quinazoline derivatives were designed and synthesized via a one - pot condensation reaction between isatoic anhydride and aromatic aldehydes with anilines using aluminum sulfate as a catalyst in refluxing ethanol . - pubmed
The entire synthesized compounds showed the potent alpha - glucosidase inhibitory activity compared with acarbose as a standard material . - pubmed
Amongst , the compounds 4h and 4i showed the strongest enzyme inhibitory potentials than the standard drug acarbose . - pubmed
AIM : Aims were to test the effectiveness of an organizational change intervention integrating smoking cessation treatment into usual alcohol and other drug ( AOD ) treatment , compared with usual care , on ( 1 ) 7 - day point prevalence abstinence ( PPA ) at 8 weeks follow - up ; ( 2 ) prolonged abstinence ; ( 3 ) cigarettes smoked per day ; ( 4 ) number of quit attempts ; and ( 5 ) offer and use of nicotine replacement therapy ( NRT ) . - pubmed
The spinal LTP was remarkably reversed by the spinal application of either BLA or gastrin - releasing peptide receptor ( GRPR ) antagonist ( PD176252 ) . - pubmed
Together , BLA was effective in inhibiting histamine - dependent and histamine - independent itches , and the mechanisms underlying these effects were involved but not limited to the inhibition of GRP - GRPR signaling in the spinal dorsal horn . - pubmed
These were : triazoles for inhibition of retinoic acid ( RA ) metabolism , AM580 and CD3254 for selective activation of respectively RA - receptor ( RAR ) and retinoid - X - receptor ( RXR ) , dithiocarbamates for inhibition of lysyl oxidase , TCDD for activation of the aryl - hydrocarbon - receptor ( AhR ) , VPA for inhibition of histone deacetylase ( HDAC ) , and PFOS for activation of peroxisome proliferator - activated receptor - alpha ( PPARalpha ) . - pubmed
In this way , 2,4 - dinitrophenol matched with the TCDD and RAR profiles , boric acid with RAR , endosulfan with PFOS , fenpropimorph with dithiocarbamates , PCB126 with AhR , and RA with triazoles and RAR profiles . - pubmed
In the absence of a cocatalyst , the as - obtained colloidal CdS - NC photocatalyst achieves a superb H2 - evolution rate of 6.86 mmol h-1 g-1 and an apparent quantum efficiency of 52.8 % under visible light . - pubmed
One - pot access to 2 - amino-3 - arylbenzofurans : direct entry to polyheterocyclic chemical space . - pubmed
As a means to make new benzofuran - embedded polycyclic structures , we established two efficient one - pot sequential coupling routes to 2 - amino-3 - arylbenzofurans and 2 - amino-3 - arylnaphtho [ 2,1 - b ] furans . - pubmed
Further ring formation ( six- and seven - membered rings ) with the resulting amine moiety at the C2 position of benzofurans was realized , leading to further expansion of benzofuran - based chemical space . - pubmed
It also allowed us to uncover the functions of hypothetical proteins , such as Sll0445 , Sll0446 , and Sll0447 , participating in photosynthesis and cell motility , and Sll1334 in regulating fatty acid biogenesis . - pubmed
Stimulation of cerebral histamine H2 receptors by clonidine . - pubmed
In order to determine the influence of copper additives on the protein digestibility , the complex formation of proteins with Cu ions under acidic conditions ( pH=2.2 ) was investigated . - pubmed
pepsin was mixed with 10 mumol ( 2.49 mg ) CuSO4 - 5H2O and dialysed against low molecular complexing agents with increasing affinity towards copper ions . - pubmed
The remaining copper ions in pepsin could only be removed by alanin and ammonium - tetramethylendithiocarbamate . - pubmed
Maximum incorporation of 3H-5 - HTP is achieved by 2 h and that of 3H-5 - HT by 20 minutes post injection . - pubmed
Results indicated that chewable isosorbide dinitrate reduced systolic blood pressure and the triple product ( systolic blood pressure X heart rate X ejection time ) significantly during rest and reduced the left ventricular ejection time corrected for heart rate both at rest and peak exercise ; no significant differences were observed in the placebo group . - pubmed
Complex I and IV subunits were more affected than complex V subunits , and there was a rough correlation with both protein size and number of lysine residues . - pubmed
BW ) resulted in a marked and sustained elevation of serum growth hormone ( GH ) and a slight and delayed increase in plasma prostaglandin E2 - M. - pubmed
delta psi dissipation requires SecA , ATP , and proOmpA. - pubmed
proOmpA which , due to the covalent addition of a folded protein to a cysteinyl side chain , is arrested during its translocation , can nevertheless cause the loss of delta psi . - pubmed
This translocation - specific reduction in delta psi is only seen in the presence of halide anions , although halide anions are not needed for proOmpA translocation per se . - pubmed
Loss of lymphoproliferative responses to antigen occurs early in infected children and precedes the loss of CD4 + helper cells and of PHA responsiveness . - pubmed
Effect of exercise training on insulin sensitivity and glucose metabolism in lean , obese , and diabetic men . - pubmed
Before and 4 - 5 days after the last training session , euglycemic hyperinsulinemic ( 40 mU.m2.min-1 ) clamps were performed at a plasma glucose of 90 mg / dl , combined with indirect calorimetry . - pubmed
Total insulin - stimulated glucose disposal ( M ) was corrected for residual hepatic glucose output . - pubmed
Insulin sensitivity was not significantly altered by training in any group , but basal hepatic glucose production was reduced by 22 % in the diabetic men . - pubmed
Superfusion of NILs with the beta - adrenergic agonist ISO ( 30 min pulse ) resulted in a rapid , sustained and concentration - dependent stimulation of the release of beta E - like immunoreactivity ( beta E - IR ) over basal as determined with an antiserum directed against the C - terminus of the beta E- ( 1 - 31 ) sequence ( 10(-6 ) M : + 145 % ; 10(-7 ) M : + 73 % ; 10(-8 ) m : + 41 % ) . - pubmed
These effects of ISO were antagonized by the competitive alpha - adrenoceptor antagonist propranolol in a concentration - dependent manner , indicating the involvement of alpha - adrenoceptors . - pubmed
The beta - related peptides released from the NILs under basal and ISO - stimulated conditions were further characterized , based on their retention times in a reversed - phase HPLC system and their reactivity with specific antisera recognizing respectively the midportion of beta E , the N - terminus of acetylated endorphins , the C - terminus of tau - endorphin ( beta E -( 1 - 17 ) ; tau E ) , or the C - terminus of alpha - endorphin ( beta E-(1 - 16 ) ; alpha E ) . - pubmed
Six smaller peaks were observed , with the characteristics of beta E-(1 - 26 ) and beta E-(1 - 27 ) ( these peptides were not resolved with the HPLC system used ) , Ac tau E , tau E , Aa alpha E , and des - tyrosine - alpha E ( DT alpha E ) , respectively . - pubmed
The IgG monoclonals LRB 21 and LRB 45 recognized a spatially close together or identical apoC - I epitope . - pubmed
Fertility alpha 2 - microglobulin reacts with 3 out of 8 lectins , which possess affinity to monosaccharides ( glucose and mannose ) and acetylamino sugar . - pubmed
The results indicate that the 7F7 antigen and the P3.58 melanoma antigen are identical , that some epitopes of this adherence molecule are differentially expressed on individual cell types and that one epitope , which is only weakly expressed on activated T cells , does not contribute to the role of the 7F7 antigen in some examples of cell - cell adherence . - pubmed
This effect was mimicked by the M2 ligand AF - DX 116 , but neither the M1 - selective antagonist pirenzepine , nor the putative M3 - muscarinic antagonist , 4 - diphenylacetoxy - N - methylpiperidine ( 4 - DAMP ) , altered ACh release . - pubmed
The beta - carbolines , which are potent ligands for benzodiazepine receptors , antagonize the pharmacological actions of benzodiazepines . - pubmed
These findings indicate that the beta - carboline functionally interacts with benzodiazepine receptors in a manner unlike that seen with typical agonists and antagonists . - pubmed
Further evidence of phencyclidine / sigma opioid receptor commonality . - pubmed
Testosterone is able to restore prolactin binding in hypophysectomized animals , as PRL treatment does . - pubmed
Uptake of the following precursors was investigated : apocytochrome c , ADP / ATP carrier and subunit 9 of the oligomycin - sensitive ATPase . - pubmed
Concentrations of added apocytochrome c exceeding the concentrations of precursor proteins synthesized in vitro by a factor of about 10(4 ) did not inhibit the transfer of ADP / ATP carrier or ATPase subunit 9 into mitochondria . - pubmed
Carbonylcyanide m - chlorophenylhydrazone , an uncoupler of oxidate phosphorylation , inhibited transfer in vitro of ADP / ATP carrier and of ATPase subunit 9 , but not of cytochrome c. - pubmed
Both follicular fluid and granulosa cell homogenate , in vitro , catabolized 125I - labelled hCG to hormone sub - units . - pubmed
The many faces of methylmercury poisoning . - pubmed
Modification of enoyl - CoA hydratase using diethyl pyrocarbonate . - pubmed
Diethyl pyrocarbonate inactivates enoyl - CoA hydratase ( L-3 - hydroxyacyl - CoA hydro - lyase , EC 4.2.1.17 ) with a second - order rate constant of 1.3 M-1 X s-1 . - pubmed
The single histidine per enzyme subunit is completely modified at a rate considerably faster than inactivation , and enzymatic activity is not restored by treatment with hydroxylamine . - pubmed
Destruction of N - terminal serine residue by periodate oxidation lowers , but does not abolish enzyme activity . - pubmed
Modification with diethyl pyrocarbonate does not lead to any gross changes in the structure of the enzyme . - pubmed
These experiments taken together show that , in contrast to other hydratases , histidine is not involved in the catalytic mechanism of enoyl - CoA hydratase , and suggest that a single residue is important for activity . - pubmed
The primary evidence for this conclusion is that ventral tegmental electrolytic or 6 - OHDA lesions which damaged specifically these fibers invariably caused : ( 1 ) a marked reduction of CA varicosities in the perifornical area ; ( 2 ) a strong reduction or loss of the anorectic response produced by perifornical injection of the presynaptically acting drugs amphetamine and mazindol ; and ( 3 ) a potentiation of the anorectic response produced by perifornical injection of the CA receptor agonists dopamine and epinephrine . - pubmed
On subjecting 2162 - fold purified enzyme preparations to polyacrylamide - gel electrophoresis , a single band of protein coincident with the zone of enzyme activity and having an apparent molecular weight of 205 000 + /- 10 000 was identified . - pubmed
Some metalloenzyme properties of the phosphatase were also studied and it was demonstrated that both Mg2 + and Zn2 + ions are necessary for hydrolytic activity . - pubmed
The sialic acid residues , however , do not appear to constitute part of the active centre of the enzyme . - pubmed
The enzyme also hydrolysed a variety of compounds having either phosphomonoester or pyrophosphate linkages and was inactivated after heating at 60 degrees C for 15 min . - pubmed
Histone , protamine and myelin basic protein significantly stimulated cyclic GMP formation , both in a time- and concentration - dependent manner . - pubmed
Removal of the extracellular / intracellular Ca2 + gradient by a Ca2 + chelator completely abolished the cyclic GMP responses elicited by histone and protamine , suggesting that influx of extracellular Ca2 + might be involved in their activation of NO synthase . - pubmed
The effects of myelin basic protein on cyclic GMP formation , however , appeared to be due to Ca2 + release from intracellular stores . - pubmed
Generation of active oxygen forms ( GAOF ) by alveolar macrophages ( AM ) and leukocytes , the role of GAOF in provoking a bronchial asthma ( BA ) attack were studied simultaneously and compared in 10 asthmatics , 10 chronics with bronchitis and 5 healthy subjects . - pubmed
GAOF was evaluated using luminol - dependent chemiluminescence . - pubmed
It appeared that in BA aggravation leukocytes generate more active oxygen forms , but AM activation surpasses that of leucocytes . - pubmed
This study noninvasively measured hemoglobin oxygen saturation in young ( n = 6 , 28 + /- 6 yrs ) , old healthy ( n = 20 , 68 + /- 7 yrs ) , and old subjects with mild peripheral vascular disease ( PVD ) ( n = 8 , 72 + /- 4 yrs ) . - pubmed
Hemoglobin oxygen saturation was measured as the difference in absorption at 760 and 850 nm light using near - infrared spectroscopy ( NIRS ) . - pubmed
Effect of epinephrine on 86Rb efflux , 45Ca outflow and insulin release from pancreatic islets perifused in the presence of propranolol . - pubmed
In the presence of 16.7 mM D - glucose , however , epinephrine lowered both 86Rb and 45Ca outflow , this coinciding with suppression of insulin release . - pubmed
These findings are compatible with the recent proposal that epinephrine interferes with the entry of Ca2 + into the B - cell , as mediated by voltage - sensitive Ca2 + channels , but do not rule out a multifactorial coupling between the occupancy of alpha 2 - adrenergic receptors and the eventual inhibition of insulin release . - pubmed
The enzyme can convert both cephalosporin acid to 7 - aminocephalosporanic acid . - pubmed
Although IgM and IgG antibodies to cholesterol were detected , the titers of IgG antibodies were low when compared with IgM. - pubmed
The anti - cholesterol vaccine , which consists of liposomes heavily laden with cholesterol as an antigen and lipid A as an adjuvant , induces antibodies that react with low density lipoproteins ( LDL ) and opsonize them for removal by liver macrophages . - pubmed
Physiological , biochemical , and EPR characterization of site - directed mutants lacking the highest or lowest potential [ 4Fe-4S ] clusters . - pubmed
The C196A and C227A mutations resulted in the full loss of the four Fe - S clusters and of the Mo - cofactor , leading to inactive enzymes . - pubmed
The EPR analysis showed that the highest redox potential [ 4Fe-4S ] cluster ( center 1 ) was selectively removed by the C263A mutation and that the C26A replacement likely eliminated the lowest potential [ 4Fe-4S ] cluster ( center 4 ) . - pubmed
In both mutants , the three remaining Fe - S clusters kept the same spectral and redox properties as in the wild type enzyme . - pubmed
Cadmium - detoxification in the earthworm Enchytraeus : specific expression of a putative aldehyde dehydrogenase . - pubmed
Northern blot analysis shows that the Ebaldh - expression is strongly enhanced by Cd , but remains unaffected by other stressors such as Zn , Hg , and H2O2 . - pubmed
buchholzi requires low intracellular concentrations of aldehydes which are known to target sulfhydryl groups thus inactivating the Cd - binding capacity of CRP . - pubmed
subtilis DnaA protein was localized in the 93 C - terminal amino acids . - pubmed
VE was measured at peak inspiratory pressures ( PIP ) of 20 , 30 , 40 or 50 cm H2O while respiratory rate ( RR ) was held constant at 20 breaths / min . - pubmed
VE was measured as RR was set at 20 , 30 , 40 , or 50 breaths / min while target PIP was held constant at 20 cm H2O. - pubmed
They showed a preference for the binding to iron - loaded hTf . - pubmed
The effect of [ 3,4 - di ( 4 - methoxyphenyl )- 5 - isoxazolyl ] acetic acid ( mofezolac ) , a potent and highly preferential inhibitor of cyclooxygenase-1 , and N -[ 2 -( cyclohexyloxy )- 4 - nitrophenyl ] methanesulphonamide ( NS-398 ) , a selective inhibitor of cyclooxygenase-2 , on DNA fragmentation and cyclooxygenase-2 mRNA expression was weak compared to the effect of indomethacin or sodium diclofenac . - pubmed
The DNA fragmentation induced by sodium diclofenac was hardly affected by the exogenous addition of 16,16 - dimethyl prostaglandin E2 but was inhibited by caspase inhibitors such as Ac - YVAD - CHO and Ac - DEVD - CHO . - pubmed
The present data provide the first evidence that NSAIDs , such as indomethacin and sodium diclofenac , cause apoptotic DNA fragmentation in cultured gastric mucosal cells , and also indicate the involvement of caspases rather than the inhibition of cellular prostaglandin synthesis in the apoptotic process . - pubmed
The accumulation of cGMP and the production of TNF - alpha were inhibited by NG - monomethyl - L - arginine ( L - NMMA ) , indicating that sCD21 activates the L - arginine pathway of NO production . - pubmed
We demonstrated that sCD21 activates NO synthase ( NOS ) since it was found to enhance the conversion of L - arginine into L - citrulline and induce the intracellular expression of inducible NOS in CD23 + monocytes . - pubmed
The increases depended on extracellular Ca2 + , were unaffected by the inositol 1,4,5 - trisphosphate ( IP3 ) inhibitor heparin or an anti - IP3 receptor antibody and were unchanged when the endoplasmic reticulum was segregated to the hemisphere opposite the injection site by centrifugation . - pubmed
Postnatal depression ' was suggested in one - third of the sample , and in two - thirds of those with'Type II ' bulimia , and was predicted by alcohol misuse , symptom severity and persistence . - pubmed
hPT cells retained GSH - dependent enzyme activities during primary culture , whereas cells that had undergone subsequent passage exhibited a loss of activities of most GSH - dependent enzymes and no longer expressed P450s or GSTs . - pubmed
CYP4A11 expression in primary cultures of hPT cells was significantly increased after treatment for 48 h with either ethanol ( 50 mM ) or dexamethasone ( 7 nM ) . - pubmed
Experiments were performed in the absence of sodium ; the presence of 2.5 mM amiloride , a potent inhibitor of the Na (+)/ H (+ ) antiport ; in the absence of bicarbonate ; and in the presence of 4 , 4 '- diisothiocyanatodihydrostilbene-2,2 '- di - sulfonic acid , disodium salt ( H ( 2 ) DIDS ) for all three activation methods . - pubmed
This suggests that the Na (+)/ H (+ ) antiport and the HCO ( 3 )(-)/ Cl (- ) exchangers are not playing a role during treatment with calcium ionophore or thimerosal , and the pH increase observed during treatment with 7 % ethanol may be dependent upon a sodium or bicarbonate flux ( or both ) into the oocyte . - pubmed
Bafilomycin A1 ( 500 nm ) , an inhibitor of vacuolar - type H (+ ) ATPases , had no effect on 7 % ethanol or thimerosal treatments , but significantly reduced the increase in intracellular pH observed during calcium ionophore treatment . - pubmed
There is evidence for accelerated amino acid substitution in Hex - t1 that has lost residues known to be associated with glucose and glucose-6 - phosphate binding . - pubmed
The enzyme 5 - lipoxygenase catalyzes the rate - limiting first two steps in the synthesis of leukotrienes from arachidonic acid . - pubmed
The regulation of this movement of soluble 5 - lipoxygenase between the cytoplasm and the nucleoplasm , as well as its impact on 5 - lipoxygenase action , leukotriene synthesis and cell function , is only now being elucidated . - pubmed
Breakthrough times of cartridges and canisters were examined by generating cyclohexane vapor in a test airflow of 30 L / min in comparison with CCl4 vapor under various test conditions with different vapor concentrations and relative humidities ( RH ) . - pubmed
The purpose of the present study was to test the hypothesis that fatty acid chain length and degree of unsaturation differ with regard to their influence on the postprandial release of cholecystokinin ( CCK ) and the subsequent increase in plasma bile acid concentrations that occur following a meal . - pubmed
The high - fat meals were enriched in oleate or palmitate , or linoleate or medium - chain triacylglycerols ( MCT ) or a blend of oleate and long - chain n-3 fatty acids . - pubmed
The postprandial increase in plasma CCK concentration was lower on the MCT meal compared with all meals and was greater following the linoleate compared with the low - fat meal . - pubmed
Plasma bile acid concentrations increased 2 - 3 - fold postprandially but the increase was lower following the MCT meal compared with the other meals and was greater on the linoleate meal compared with the low - fat meal . - pubmed
In conclusion , meals rich in linoleate are a potent stimulus for CCK release and lead to prolonged elevations of plasma bile acids and meals containing MCT inhibit CCK release and the subsequent increase in plasma bile acid concentrations . - pubmed
Antisense oligonucleotides to SYT - SSX mRNA rapidly and drastically decreased cyclin D1 and subsequently inhibited cell growth . - pubmed
The decrease in cyclin D1 expression , which occurred shortly after inhibition of SYT - SSX expression , was found to be primarily dependent on an increased degradation of the cyclin D1 protein , as assessed by pulse - chase experiments using [ ( 35 ) S ] methionine . - pubmed
The promoters of the xynA and xynB genes have been sequenced ; both present consensus sequences for the binding of the carbon catabolite repressor CreA , but otherwise show substantial differences . - pubmed
In contrast , xynB is broadly induced by all these carbon sources ; this may be related to the presence of several XlnR boxes . - pubmed
The first enzyme in the biosynthetic pathway of most fructans is sucrose : sucrose fructosyl transferase ( SST ) . - pubmed
Soluble invertase activity found in the SST preparations was less sensitive to KCl and more sensitive to choline chloride than was SST . - pubmed
CuZnSOD is an efficient catalyst of rHCaBP S - nitrosation , and the mechanism of CuZnSOD - catalyzed S - nitrosation involves reduction of the active - site Cu ( II ) by a number of the five free thiols in rHCaBP , giving rise to thiyl radicals . - pubmed
S - nitrosation is rapidly gaining recognition as a major form of protein posttranslational modification , and the efficient S - nitrosation of CaBP by CuZnSOD / GSNO is speculated to be of neurochemical importance given that CaBP and CuZnSOD are abundant in neurons . - pubmed
We examined the potential possibility of an interaction between nicotine , a major component of cigarette smoke , and angiotensin II ( Ang II ) , which plays an important role in the pathogenesis of cardiovascular diseases characterized by Ang II type 1 ( AT1 ) receptor - mediated abnormal growth of vascular smooth muscle cells ( VSMC ) and fibroblasts . - pubmed
The nicotine - induced DNA synthesis was not affected by valsartan , an AT1 receptor - specific blocker , or PD123319 , an Ang II type 2 ( AT2 ) receptor - specific antagonist . - pubmed
Nicotine or Ang II stimulation rapidly increased extracellular signal - regulated kinase ( ERK ) activation , tyrosine- and serine - phosphorylation of signal transducer and activator of transcription ( STAT ) 1 and STAT3 , and p38 mitogen - activated protein kinase ( p38 MAPK ) , in both cell types . - pubmed
Interestingly , co - administration of nicotine and Ang II at lower doses , which did not affect cell growth , induced DNA synthesis and c - fos expression accompanied by enhancement of ERK , STAT , and p38MAPK activity . - pubmed
PD98059 , a mitogen - activated protein kinase / ERK kinase inhibitor , or SB23058 , a p38MAPK inhibitor , significantly attenuated the vasotrophic effect of nicotine and Ang II . - pubmed
CONCLUSIONS : These results suggest that nicotine exerts a growth - promoting effect on vascular cells and enhances the Ang II - induced vasotrophic effect , which is at least partly mediated by the activation of ERK , STAT , and p38MAPK . - pubmed
Although TRP14 contains five cysteines , only the two Cys residues in its WCPDC motif were exposed and redox sensitive . - pubmed
Biochemical characterization of TRP14 suggested that , like Trx1 , TRP14 is a disulfide reductase ; its active site cysteine is sufficiently nucleophilic with the pK(a ) value of 6.1 ; and its redox potential ( -257 mV ) is similar to those of other cellular thiol reductants . - pubmed
However , although TRP14 reduced small disulfide - containing peptides , it did not reduce the disulfides of known Trx1 substrates , ribonucleotide reductase , peroxiredoxin , and methionine sulfoxide reductase . - pubmed
Brevetoxins were detected by radioimmunoassay in methanolic extracts of blood within 1 h post intraperitoneal ( ip ) administration . - pubmed
The analytes were then identified and quantitatively determined by gas chromatography - mass spectrometry ( GC - MS ) via on - line derivatization in the injection - port using a large - volume ( 10 microl ) sample injection with tetrabutylammonium ( TBA ) salts . - pubmed
Sixty minutes after the administration of the beverage , the angiotensin I - induced pressor response decreased to +45+/-7 mmHg at 60 min ( p<0.05 ) , whereas no significant change was detected in the norepinephrine - induced pressor response . - pubmed
Familial hemiplegic migraine ( FHM ) is a rare monogenic subform caused by mutations in the calcium channel gene CACNA1A or the Na (+)/ K (+)- ATPase gene ATP1A2 . - pubmed
Anti - CD20 monoclonal antibody ( rituximab ) in the treatment of pemphigus . - pubmed
Arachidonic acid [ AA , 20:4(n-6 ) ] , EPA [ 20:5(n-3 ) ] , and DHA [ 22:6(n-3 ) ] antagonized the depression of LDLr gene expression by 25OH - cholesterol and increased LDLr protein abundance 1- to 3 - fold ( P & lt ; 0.05 ) , but had no significant effects on LDLr mRNA levels . - pubmed
Oleic ( 18:1 ) , linoleic ( 18:2 ) , and alpha - linolenic acids [ 18:3(n-3 ) ] did not significantly affect LDLr expression . - pubmed
The present results suggest that AA , EPA , and DHA increase LDLr protein levels , and that ACAT plays a role in modulating the effects of AA on LDLr protein levels . - pubmed
Furthermore , the effects of the fatty acids appeared to be independent of any change in SREBP-1 protein . - pubmed
Of these 5,437 ( 79.5 % ) showed sinus rhythm ( SR ) and 1,398 ( 20.5 % ) showed AF . - pubmed
CONCLUSIONS : BioGlue appears to be an effective adjunct in preventing postoperative CSF leaks after transsphenoidal surgery . - pubmed
Supramolecular metal ion assemblies are deposited from their solutions onto highly orientated pyrolytic graphite ( HOPG ) substrates to be imaged by scanning tunnelling microscopy ( STM ) . - pubmed
The renal antioxidant enzymes such as superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione peroxidase ( GPx ) activities and level of reduced glutathione ( GSH ) were declined ; level of malondialdehyde ( MDA ) was elevated in the cisplatin alone treated group . - pubmed
CONCLUSIONS : Granulocyte colony - stimulating factor ( G - CSF ) , all - trans retinoic acid ( ATRA ) , and vaccines met 2 of 3 criteria for an association with SS . - pubmed
There are sufficient data and a plausible pharmacologic mechanism for G - CSF and ATRA . - pubmed
Our analysis showed MELD score , bilirubin , ALT levels and white cell counts preorthotopic liver transplantation are important predictive factors for outcome . - pubmed
Accumulation of hypoxia - inducible factor-1alpha through a novel electrophilic , thiol antioxidant - sensitive mechanism . - pubmed
15 - deoxy - Delta ( 12,14 )- prostaglandin - J ( 2 ) ( 15d - PGJ ( 2 ) ) is a peroxisome - activated proliferator receptor - gamma ( PPARgamma ) agonist which contains an alpha , beta - unsaturated electrophilic ketone involved in nucleophilic addition reactions to thiols . - pubmed
15d - PGJ ( 2 ) induced stabilization of HIF-1alpha protein , without affecting HIF-1alpha mRNA levels or proteasome activity , leading to its nuclear accumulation and activation of HIF - induced transcription . - pubmed
Accumulation of HIF-1alpha was unaffected by selective PPARgamma blockade nor mimicked by the PPARgamma agonists ciglitazone and 9,10 - dihydro-15d - PGJ ( 2 ) . - pubmed
agents that act as thiol reducing agents and/or increase the GSH content ) , but not reactive oxygen species ( ROS ) scavengers , prevented 15d - PGJ ( 2 )-induced HIF-1alpha accumulation whereas the inhibitor of GSH synthesis buthionine sulfoximine cooperated with 15d - PGJ ( 2 ) to accumulate HIF-1alpha . - pubmed
Finally , HIF-1alpha expression was increased by the electrophilic alpha , beta - unsaturated compounds acrolein and PGA ( 2 ) , but not by 9,10 - dihydro-15d - PGJ ( 2 ) , which lacks the electrophilic cyclopentenone moiety . - pubmed
Taken together , these results point out to a new mechanism to increase pharmacologically the cell levels of HIF-1alpha through the electrophilic reaction of alpha , beta - unsaturated ketones with thiol groups . - pubmed
The primary efficacy end point was glycemic control as determined by changes in glycosylated hemoglobin ( HbA(1c ) ) from baseline to study end between those receiving combination MER+S treatment and those receiving sulfonylurea monotherapy . - pubmed
There were significant decreases from baseline in mean fasting plasma glucose ( FPG ) levels by the end of week 1 and in mean HbA(1c ) levels by week 8 in each MER+S group ( both , P & lt ; 0.001 ) . - pubmed
The mean ( 95 % CI ) changes from baseline to study end in the combined MER+S groups ( HbA(1c ) , -0.74 % [ -0.85 % to -0.64 % ] ; FPG , -12.9 [ -17.1 to -8.7 ] mg / dL ) were significantly different from the sulfonylurea monotherapy group ( HbA(1c ) , 0.08 % [ -0.08 % to 0.25 % ] ; FPG , 15.5 [ 8.2 to 22.8 ] mg / dL ; P & lt ; 0.001 ) . - pubmed
The unadjusted 6 - month medical costs for the newer agents ranged from $ US 2715 for paroxetine CR to $ US 6042 for duloxetine . - pubmed
Remarkable seasonal consistency was found in the amount of storage reserves available for nocturnal carboxylation , a consequence of predominantly daytime export of carbohydrate in spring and summer whilst nocturnal export was the major sink for carbohydrate in autumn and winter . - pubmed
In this study , the following topics regarding SN identification in lung cancer patients will be introduced : ( 1 ) devices for SN identification using a radioisotope tracer ; ( 2 ) movement of Tc-99 tin colloid after injection ; ( 3 ) characteristics of patients in whom SNs could not be identified ; ( 4 ) results of ex vivo SN identification ; ( 5 ) reliability of in vivo SN identification ; ( 6 ) algorithm for reducing mediastinal lymph node dissection ; ( 7 ) SN identification using SPECT / CT ; ( 8) differences in SN identification between large and small radioisotope particles ; ( 9 ) size of metastatic and nonmetastatic mediastinal lymph nodes in non - small cell lung cancer ; ( 10 ) SN navigation segmentectomy for clinical stage IA non - small cell lung cancer ; and ( 11 ) lymphatic flow at segmental lymph nodes . - pubmed
The K(i ) results reveal a surprising possibility that VKOR in some bird species are easily inhibited by warfarin . - pubmed
At day 28 , the proportion of CD34 + cells , myocardial BrdU immunohistochemical stain , myocardial capillary density , myocardial fibrosis and cardiac function was detected among the 3 groups . - pubmed
RESULTS : In Group G , the proportion of CD34 + cells and cardiac function was significantly higher than groups N and C , and BrdU positive cells in myocardium were higher than those other groups . - pubmed
RESULTS : We found that dietary supplementation of 10 g MLP kg(-1 ) or 1 g Q3MG kg(-1 ) in high - fat diet effectively suppressed blood glucose levels . - pubmed
Moreover , the production of chemokine - containing HS - TEX could be inhibited by ATP inhibitor , calcium chelator , and cholesterol scavenger , indicating that the mobilization of chemokines into exosomes was ATP- and calcium - dependent and via a lipid raft - dependent pathway . - pubmed
Socio - demographic information was collected via a self - administered instrument ( gender , age , school grade , economic level ( EL ) and family - head 's EL ) , SBC for PA , behavioural factors ( smoking , alcohol and stress ) and PA barriers . - pubmed
Modeling the kinetics of digoxin absorption : enhancement by P - glycoprotein inhibition . - pubmed
An increase in the area under the curve ( AUC ) after oral digoxin due to coadministration of drugs known as P - glycoprotein ( P - gp ) inhibitors has been reported in several studies , but there is very little information on the rate of absorption after P - gp inhibition . - pubmed
However , even the effect of the highest dose of naltrexone ( 20 mg / kg ) was incomplete , which is in contrast to the effect of the NMDA receptor antagonist MK-801 , which has been tested in the same model and found to completely prevent the incidence of allodynia at a dose of 0.5 mg / kg . - pubmed
Thus , NMDA receptor antagonists may contribute to the prevention of tissue damage by antagonizing the excitotoxic action of glutamate and/or aspartate released by ischemia into the spinal cord . - pubmed
Catalytic enantioselective one - pot aziridoarylation reaction of aryl cinnamyl ethers has been demonstrated in detail . - pubmed
trans-3 - Amino-4 - arylchroman is an advanced intermediate for the synthesis of chromenoisoquinoline compounds such as doxanthrine , a potent and selective full agonist for the dopamine - D ( 1 ) receptor . - pubmed
Thrombin generation in PPP was assessed using calibrated automated thrombogram . - pubmed
TiO2 nanoparticles induce insulin resistance in liver - derived cells both directly and via macrophage activation . - pubmed
We hypothesized that despite being considered " inert " as bulk material , TiO ( 2 ) NPs may impair insulin responses in liver - derived cells , either indirectly by inflammatory activation of macrophages , and/or by directly interfering with insulin signaling . - pubmed
Furthermore , direct exposure of Fao cells to TiO ( 2 ) results in activation of the stress kinases JNK and p38MAP kinase , and in induction of insulin resistance at the signaling and metabolic levels . - pubmed
Total collagen and testosterone levels peaked at 12 months of age . - pubmed
Breeds were ranked identically for total collagen and testosterone levels at 12 months . - pubmed
The increased synthesis of collagen appears to be influenced by testosterone or some other event associated with puberty . - pubmed
When stratified by PSA level of & lt;=10 ng / mL vs. & gt;10 ng / mL , Gleason score , or androgen deprivation therapy , no statistically significant differences in bPFS were discerned between the two EBRT regimens . - pubmed
The homogeneous alignments of helical rod - like polysilanes on a rubbed polyimide alignment layer were investigated by polarized optical microscopy ( POM ) and atomic force microscopy ( AFM ) analyses . - pubmed
The POM and AFM observations determined that polysilanes with a series of aliphatic side chains helically arranged around the main chains were tilted to the right and left by 33 from the rubbing direction when the handedness of the side - chain helical array is left and right , respectively . - pubmed
Infrared ( IR ) and nuclear magnetic resonance ( NMR ) spectroscopy have revealed that semi - fluorinated ( SF ) acids containing a perfluorooctyl group ( C8F17 ) as a terminal segment exist also as hydrogen - bonded dimers , which are the units of inter- and intramolecular movements in their liquids and CCl4 . - pubmed
In fact , low intramolecular movements for SF - acid were confirmed by 13C - NMR T1 measurements . - pubmed
Immuno fluorescent studies also revealed the extracellular filamentous actin organization over the scaffolds , where cell adhesion and proliferation was found to be higher with increase in PGA content , which is a hydrophilic polymer . - pubmed
Against this background , estrogen - alone effects on joint symptoms were examined in post hoc analyses in the Women 's Health Initiative randomized , placebo - controlled , clinical trial . - pubmed
Spearman 's rank correlation was used to determine the association of the five anthropometric indices with metabolic parameters including fasting plasma glucose , triglyceride , high density lipoprotein and blood pressure . - pubmed
Sesterterpene glycinyl - lactams : a new class of glycine receptor modulator from Australian marine sponges of the genus Psammocinia . - pubmed
Bioassay guided fractionation of three southern Australian marine sponges of the genus Psammocinia , selected for their ability to modulate glycine - gated chloride channel receptors ( GlyRs ) , yielded the rare marine sesterterpenes ( -)-ircinianin ( 1 ) and ( -)- ircinianin sulfate ( 2 ) , along with the new biosynthetically related metabolites ( -)- ircinianin lactam A ( 3 ) , ( -)- ircinianin lactam A sulfate ( 4 ) , ( -)- oxoircinianin ( 5 ) , ( -)- oxoircinianin lactam A ( 6 ) and ( -)- ircinianin lactone A ( 7 ) . - pubmed
The discovery and characterization of sesterterpenes 1 - 8 , and in particular the glycinyl - lactams 3 and 6 , provide valuable new insights into GlyR pharmacology . - pubmed
We focus our attention on multiple repeats of one amino acid ( homorepeats ) and create a new database ( named HRaP , at http://bioinfo.protres.ru/hrap/ ) of occurrence of homorepeats and disordered patterns in different proteomes . - pubmed
HRaP is aimed at understanding the amino acid tandem repeat function in different proteomes . - pubmed
SH3P2 also binds to phosphatidylinositol 3 - phosphate ( PtdIns3P ) and functions downstream of the phosphatidylinositol 3 - kinase ( PtdIns3 K ) complex . - pubmed
Our study aimed to investigate whether PKC ( protein kinase C)-NF - kappaB ( nuclear factor kappaB ) is involved in CCL2 - induced regulation of voltage - gated sodium Nav1.8 currents and expression . - pubmed
Compared with the control , significant change in Nav1.8 current density was observed when the CCR2 inhibitor INCB3344 ( 10 nM ) was applied . - pubmed
Furthermore , inhibition of PKC by AEB071 significantly eliminated CCL2 - induced elevated Nav1.8 currents . - pubmed
Moreover , p65 expression was significantly higher in CCL2 - induced neurons ( P<0.05 ) , and was reversed by treatment with INCB3344 and AEB071 . - pubmed
CONCLUSIONS : Our results suggest that Glycine max may change the expression level of CYPs , especially CYP3A4 and CYP1A 7 , involved in biotransformation of xenobiotics ( drugs , procarcinogens ) and may participate in clinically significant interactions with drugs metabolized by these enzymes . - pubmed
Additional studies indicated the production of reactive oxygen species accompanied of decreases in vitamin E and GSH contents and of glutathione transferase activity . - pubmed
Estrogens are known that they may produce breast cancer by actions on ER and also as chemical carcinogens , as a consequence of their oxidation leading to reactive metabolites . - pubmed
Duplex staining for proliferating cell nuclear antigen and alpha - smooth muscle actin ( alpha - SMA ) , as well as MTT and flow cytometry assays , were performed to measure fibroblast proliferation . - pubmed
Curcumin treatment inhibited fibroblast proliferation and the cell cycle was arrested in the G1 phase . - pubmed
Curcumin treatment upregulated the expression of PPAR - gamma and downregulated the expression of p - Smad2 / 3 . - pubmed
These results suggest that curcumin treatment ameliorates renal fibrosis by reducing fibroblast proliferation and ECM accumulation mediated by PPAR - gamma and Smad - dependent TGF - beta1 signaling . - pubmed
Crustins are cationic cysteine - rich antimicrobial peptides ( AMPs ) that contain multiple domains ( glycine - rich , cysteine - rich , or proline - rich ) at the N - terminus and whey acidic protein ( WAP ) domains at the C - terminus . - pubmed
The synthesized WAP domain of MjCru I-1 but not synthesized Cys - rich domain has antibacterial and agglutinative activities . - pubmed
During the first stage ethanol is oxidized into acetaldehyde , under the action of alcohol dehydrogenase . - pubmed
Micro - agar larval development test , MTT test of cytotoxicity , and biotransformation study coupled with Ultra high performance liquid chromatography - tandem mass spectrometry ( UHPLC - MS / MS ) technique were used for this purpose . - pubmed
The objective of this study was to increase the potency and durability of vaccine - induced immunity against B cell lymphoma by combining alpha - galactosylceramide ( alpha - GalCer)-loaded tumor cell vaccination with an agonistic antibody targeting the immune checkpoint molecule 4 - 1BB ( CD137 ) . - pubmed
RESULTS : Aerobic training significantly increased peak oxygen uptake ( VO2 ) ( 18.1 + - 3.0 vs. 22.9 + - 2.8 mL / kg / min ) , decreased the change in muscle oxygen saturation from rest to submaximal and peak exercise ( SmO2 ; 2.4 + - 5.7 vs. -7.0 + - 3.4 % ) , and increased the relative change in deoxygenated hemoglobin / myoglobin concentration from rest to submaximal ( -1.5 + - 2.3 vs. 3.0 + - 3.6 mumol / L ) and peak exercise ( 1.1 + - 4.5 vs. 8.2 + - 3.5 mumol / L ) . - pubmed
HSCT using alternative donors with post - transplantation cyclophosphamide ( PT / Cy ) for GVHD prophylaxis has been performed for hematologic malignancies with engraftment , GVHD , and TRM comparable with that seen with HLA - matched related donors . - pubmed
Furthermore , separation and detection of underivatized optical isomers of amphetamine , cathinone , nor - mephedrone , and pregabalin using UFCE / MS can be achieved with an analysis time of less than two minutes . - pubmed
Resolutions of 1.3 , 3.7 and 3.8 were achieved for pregabalin , cathinone and nor - mephedrone , respectively , under UFCE / MS conditions . - pubmed
The samples were extracted once with acetonitrile , concentrated by evaporation and injected into the LC - MS / MS system . - pubmed
Dual Energy CT Pulmonary Angiography with 6 g Iodine - A Propensity Score - Matched Study . - pubmed
CONCLUSION : Dual - energy CTPA with advanced monoenergetic reconstruction is feasible with 6 g iodine and allows for the diagnosis and safe exclusion of central , lobar , and segmental PE . - pubmed
Infusion reactions during infliximab treatment are not associated with IgE anti - infliximab antibodies . - pubmed
OBJECTIVES : Controversy exists on the role of IgE antidrug antibodies ( IgE - ADA ) in infusion reactions ( IR ) on infliximab treatment , partly due to the lack of a positive control used for assay validation . - pubmed
Kissorphin ( KSO ) is a new peptide derived from kisspeptin-10 . - pubmed
Moreover , prior administration of the NPFF receptor antagonist RF9 ( 10 nmol/300 muL , i.v . ) - pubmed
Simulated bioavailability of phosphorus from aquatic macrophytes and phytoplankton by aqueous suspension and incubation with alkaline phosphatase . - pubmed
Haloacetic Acid Water Disinfection Byproducts Affect Pyruvate Dehydrogenase Activity and Disrupt Cellular Metabolism . - pubmed
Previous studies of monoHAA - induced genotoxicity and cell stress demonstrated that the toxicity was due to inhibition of glyceraldehyde-3 - phosphate dehydrogenase ( GAPDH ) , leading to disruption of cellular metabolism and energy homeostasis . - pubmed
In this study , we evaluated the effects of mono - , di - , and tri - HAAs on cellular GAPDH enzyme kinetics , cellular ATP levels , and pyruvate dehydrogenase complex ( PDC ) activity . - pubmed
The monoHAAs , iodoacetic acid and bromoacetic acid , were the strongest inhibitors of GAPDH and greatly reduced cellular ATP levels . - pubmed
Chloroacetic acid , diHAAs , and triHAAs were weaker inhibitors of GAPDH and some increased the levels of cellular ATP . - pubmed
HAAs also affected PDC activity , with most HAAs activating PDC . - pubmed
The primary finding of this work is that mono- versus multi - HAAs address different molecular targets , and the results are generally consistent with a model in which monoHAAs activate the PDC through GAPDH inhibition - mediated disruption in cellular metabolites , including altering ATP - to - ADP and NADH - to - NAD ratios . - pubmed
The monoHAA - mediated reduction in cellular metabolites results in accelerated PDC activity by way of metabolite - ratio - dependent PDC regulation . - pubmed
DiHAAs and triHAAs are weaker inhibitors of GAPDH , but many also increase cellular ATP levels , and we suggest that they increase PDC activity by inhibiting pyruvate dehydrogenase kinase . - pubmed
Different techniques have been used to manage PAC - induced pulmonary artery rupture , including double lumen endotracheal tube ( DLT ) , bronchial blockers , pulmonary artery embolization , thoracotomy with hematoma evacuation , and extracorporeal life support for ventilation ( ECLS ) . - pubmed
The suitability of the device for bioanalysis and bioimaging was demonstrated by monitoring the degradation of a thin poly ( ester amide ) film with the enzyme alpha - chymotrypsin using LAPS . - pubmed
In this work , the interactions between MT2 - MMP and common trivalent metal ions , including aluminum ( Al3 + ) and ferrum ( Fe3 + ) ions , were investigated . - pubmed
Enzymatic detection revealed that Al3 + and Fe3 + strongly inhibited the MT2 - MMP . - pubmed
Fluorescence spectrography elucidated a static quenching interaction between the negatively charged amino acids on MT2 - MMP and the inhibitory trivalent metal ions , indicating that a stable complex was formed between MT2 - MMP and metal ions . - pubmed
Dosage forms of Genentech BCS class II drugs , GDC-0810 , GDC-0941 , and compound A , were tested by starting the dissolution in either pH 1.6 or pH 4.0 media then converting to FaSSIF after 30 min . - pubmed
One pot ligand exchange method for a highly stable Au - SBA-15 catalyst and its room temperature CO oxidation . - pubmed
Using the method of beta - cells detection with antibodies to insulin , we demonstrated that streptozotocin reduced the number of beta - cells and impaired their morphological structure . - pubmed
However , shikimate is not catalytically involved in the reactions of the monolignol pathway , and is only needed for pathway enzymes to recognize their main substrates . - pubmed
After CS exposure , tissue slides were made from uterine and ovarian samples and examined after hematoxylin and eosin staining . - pubmed
Furthermore , during oral glucose and insulin tolerance tests , FO from dyslipidaemic dams exhibited altered glucose tolerance and insulin sensitivity ( P & lt ; 0.05 ) when compared with FO from CTL dams . - pubmed
Switching to bupropion was strongly dominated by bupropion augmentation at an ICER of -$640 / remission ( 95 % CI , -$5,770 to $ 3,008 ) . - pubmed
The ICER for the aripiprazole augmentation versus switching to bupropion was $ 1,074 / remission ( 95 % CI , $ 47 to $ 5,022 ) , and the ICER for aripiprazole augmentation versus bupropion augmentation was $ 5,094 / remission ( 95 % CI , -$34,027 to $ 32,774 ) . - pubmed
In such conditions , rocuronium remains able to cause immunoglobulin E cross - linkage and the anaphylaxis mechanism can continue . - pubmed
Intradermic skin tests confirmed rocuronium immunoglobulin E - mediated anaphylaxis but also showed intradermal injection of mixing in equal molecular ratio of sugammadex with rocuronium preventing rocuronium anaphylactic skin reaction . - pubmed
Based on the physics understanding of the resistive switching , the measured NDR behavior is believed to be associated with the partially unipolar reset effect , which is due to the recombination between oxygen vacancies and the thermally released oxygen ions from the RuO2 interface layer . - pubmed
The nitrate reductase activity was the highest in the C+ N grown cells and the lowest activity was found in the glutamate grown cells . - pubmed
[ Role of PPAR - gamma - regulated autophagy in genistein - induced inhibition of hepatic stellate cell activation ] . - pubmed
OBJECTIVE : To investigate the inhibitory effect of genistein on activation of hepatic stellate cells ( HSCs ) in vitro and the role of the autophagy pathway regulated by PPAR - gamma in mediating this effect . - pubmed
The cells pretreated with 3 - MA prior to genistein treatment showed significantly increased protein expressions of -SMA and 1 ( I ) collagen compared with the cells treated with genistein only ( P & amp;lt ; 0.05 ) . - pubmed
CONCLUSIONS : PPAR - gamma- regulated autophagy plays an important role in mediating genistein - induced inhibition of HSC activation in vitro . - pubmed
Triglyceride deposit cardiomyovasculopathy ( TGCV ) is a phenotype primarily reported in patients carrying genetic mutations in PNPLA2 encoding adipose triglyceride lipase ( ATGL ) which releases long chain fatty acid ( LCFA ) as a major energy source by the intracellular TG hydrolysis . - pubmed
Patients with I - TGCV , of which etiologies and causes are not known yet , suffered from adult - onset severe heart disease , including heart failure and coronary artery disease , associated with a marked reduction in ATGL activity and myocardial washout rate of LCFA tracer , as similar to those with P - TGCV . - pubmed
Discovery of a Lead Triphenylethanamine Cholesterol Ester Transfer Protein ( CETP ) Inhibitor . - pubmed
Lead optimization of the diphenylpyridylethanamine ( DPPE ) and triphenylethanamine ( TPE ) series of CETP inhibitors to improve their pharmaceutical profile is described . - pubmed
RESULTS : Our data showed that EXE promoted hepatic autophagy via activation of canonical autophagy signaling pathways via mediating microtubule - associated protein B - light chain 3 II ( LC3 - II ) , autophagy protein 7 ( ATG7 ) , phosphorylated adenosine mono phosphate - activated protein kinase ( p - AMPK ) , CATHEPSIN L , lysosome - associated membrane protein 2 ( LAMP2 ) , and a reduction in p62 . - pubmed
Compared with the CON group , the ITM group showed decrease in ( P < 0.05 ) egg production , eggshell strength , eggshell palisade layer , palisade layer ratio , serum estrogen , luteinizing hormone , glycosaminoglycan concentration , and carbonic anhydrase activity and increase in ( P < 0.05 ) egg loss and mammillary layer ratio . - pubmed
In this study , polystyrene microplastic particles ( PSMPs ) with a size of 0.047 mum were selected as the representative of microplastics . - pubmed
Soil physicochemical properties play important roles in sorption of PSMPs , especially the content of soil organic carbon . - pubmed
The sorption capacity was positively correlated with soil organic carbon ( R2 = 0.999 ) and Fe2O3 content ( R2 = 0.967 ) , while the sorption of clay showed opposite trend ( R2 = 0.952 ) . - pubmed
The origin of PNH is the somatic mutation in the X - linked phosphatidylinositol glycan class A ( PIG - A ) gene located on Xp22 : this condition leads to the production of clonal blood cells with a deficiency in those surface proteins that protect against the lytic action of the activated complement system . - pubmed
In the presence of heat stress , the intracellular IAA content showed a significant increase in the two LeDnaJ07 overexpression strains but only a slight change in the YS55 wild type strain . - pubmed
OBJECTIVES : We established a novel mitochondrial / cytosolic purification pipeline for complimentary GC - TOF - MS and 1H - NMR metabolomics using robust , commercially available fractionation strategies . - pubmed
Apoptosis and intracellular reactive oxygen species ( ROS ) induced by PLF were accessed using high - content analysis with florescent staining . - pubmed
The ability of PLF to scavenge free radicals was evaluated by 2,2 - diphenyl-1 - picrylhydrazyl ( DPPH ) assay . - pubmed
Nlrp3 inflammasome elicit out an inflammatory response by emission of pro inflammatory cytokines by ROS ( reactive oxygen species ) production , mobilization of K + efflux and Ca2 + and by activation of lysosome destabilization that eventually causes pyroptosis , a programmed cell death process . - pubmed
Morphine-3 - glucuronide upregulates PD - L1 expression via TLR4 and promotes the immune escape of non - small cell lung cancer . - pubmed
M3 G activated the PI3 K and the NFkappaB signaling pathways , and this effect was antagonized by a TLR4 pathway inhibitor . - pubmed
Repeated M3 G intraperitoneal injections promoted LLC tumor growth and lung metastasis through the upregulation of tumor expressed PD - L1 and the reduction of CTL in the tumor microenvironment . - pubmed
Conclusions : M3 G specifically activated TLR4 in NSCLC cells and upregulated PD - L1 expression through the PI3 K signaling pathway , thereby inhibiting CTL cytotoxicity and finally promoting tumor immune escape . - pubmed
In this study , the influence of headspace pressure ( HP ; 20 - 100 kPa ) and organic loading rate ( OLR ; 10 - 30 g / L ) on H2 and volatile fatty acid ( VFA ) yield were investigated . - pubmed
More than 79 % generated H2 was consumed through homoacetogenesis pathway when HP was 100 kPa , and lowing HP could effectively promote the accumulation of H2 ( increased by at least 2 times ) . - pubmed
Even though consumed H2 through homoacetogenesis was related to OLR and HP , the lower HP was more likely to reduce this part H2 consumption , especially under 10 g / L condition . - pubmed
VIP - induced lipolysis and adenyl cyclase activity are not affected by phenoxybenzamine . - pubmed
VIP - induced lipolysis is inhibited by propranolol but VIP - induced adenyl cyclase activity is not . - pubmed
Triphenylmethane dyes as inhibitors of reverse transcriptase , ribonucleic acid polymerase , and protein synthesis . - pubmed
A comparison between skin tests , inhalation tests , specific IgE and basophil histamine release measurements . - pubmed
Nonspecific phosphatase activity and Ca2 + -dependent ATPase activity were not inhibited by Con A treatment of the cells . - pubmed
Abnormal lipoprotein - lipase - mediated plasma triglyceride removal in untreated diabetes mellitus associated with hypertriglyceridemia . - pubmed
An abnormality in the interaction of lipoprotein lipase with endogenous circulating plasma lipoprotein triglyceride has been demonstrated in untreated diabetes . - pubmed
These diabetics have a decreased maximal removal capacity for plasma triglyceride ( 27.0 mg TG / kh / hr ) and increased Km ( 390 mg / dl ) for endogenous plasma triglyceride - lipoprotein lipase interaction compared to that found in nondiabetic hypertriglyceridemic subjects ( Vmax , 32.0 ; km , 157 ) . - pubmed
The presence of free intrinsic factor in the small intestine has considerable physiological significance in maintaining the enterophepatic circulation of vitamin B12 . - pubmed
The exact role of ovarian sex steroids in the neuroendocrine regulation of thyrotropin ( TSH ) release in women can only be accurately assessed in the absence of any considerable ovarian sex steroid feedback upon the hypothalamic - pituitary unit . - pubmed
Compared to the control conditions , serum E2 and P4 concentrations markedly increased ( p less than 0.001 ) following oral E2 or E2 / P4 treatments . - pubmed
Total triiodothyronine ( T3 ) and thyroxine ( T4 ) concentrations and free T3 and T4 equivalents remained unchanged during E2 and E2 / P4 regimens . - pubmed
Since the TSH pulse attributes ( pulse amplitudes , frequencies , interpulse intervals , mean TSH concentrations , by Cluster pulse algorithm ) did not significantly change during E2 and E2 / P4 replacements , the episodic character of TSH secretion virtually remained unchanged by sex steroid replacements. - pubmed
Effects of calcium and potassium extracellular ionic concentration changes on the hippocampal CA1 activity of purinergic drugs . - pubmed
The data demonstrate a direct intrinsic activity of potassium ions , and suggest a possible direct interaction between adenosine receptors and potassium transneuronal fluxes . - pubmed
Phorbol ester and phospholipase C - mediated differentiated thyroid function in vitro : the effects of protein kinase C inhibition and downregulation . - pubmed
, 12 - O - tetradecanoylphorbol 13 - acetate ( TPA ) , inhibit TSH - stimulated iodide organification in vitro implying a role for protein kinase C ( PKC ) in the regulation of differentiated thyroid function . - pubmed
To further explore the PKC dependence of this action of TPA , we studied the effects of PKC inhibition and downregulation on phorbol - mediated differentiated thyroid function in vitro . - pubmed
Exogenous PLC ( 1 U / mL ) could mimic these effects of TPA . - pubmed
The PKC inhibitor , 1 -( 5 - isoquinolinesulfonyl )- 2 - methylpiperazine ( H7 ) inhibited TSH - stimulated iodide organification at concentrations exceeding 10 microM. - pubmed
After 24 h of TPA treatment to induce PKC downregulation , no recovery of TSH - stimulated iodide organification was observed , suggesting that the effects of TPA were irreversible . - pubmed
These studies indicate that the effects of TPA and PLC on differentiated thyroid function are mediated , at least in part , by PKC . - pubmed
Intracerebroventricular infusions of insulin ( 4 and 40 micrograms / min for 30 min ) increased plasma AVP concentrations dose - dependently without hypoglycaemia , but decreased hypothalamic and pituitary contents of AVP . - pubmed
In the presence of 5 mM glucose the preproinsulin mRNA content was increased by 10(-8 ) M Met - enkephalin to 198 % ( p less than 0.001 ) and by Leu - enkephalin to 145 % ( p less than 0.001 ) of the control without enkephalins . - pubmed
The preproinsulin mRNA content was raised in the presence of 5 mM glucose by 10(-10 ) M significant . - pubmed
The biosynthesis of ( pro)insulin was strongly decreased by actinomycin D at the glucose and enkephalin concentrations tested . - pubmed
The role of the amino acid in position 31 of cholecystokinin CCK26 - 33 in the recognition of central and peripheral receptors was investigated by replacement of methionine-31 by amino acids with side chains of various chemical nature . - pubmed
Interestingly , when the amine function in the side chain of the ornithine-31 was protected by a benzyloxycarbonyl group , an unusual high affinity for pancreatic binding sites was observed and the related analogue proved to be a new peripheral CCK antagonist . - pubmed
After 4 hours incubation in potassium cyanide and 2 - deoxyglucose , there was a 43 % increase in beta - adrenergic receptor number , 41 % decrease in adenosine triphosphate , and minimal morphological change in myocytes . - pubmed
Thus : beta - adrenergic receptor density increases during relatively early stages of injury in metabolically impaired myocytes with reduced adenosine triphosphate levels and decreases subsequently , after the myocytes become irreversibly injured ; the increased beta - adrenergic receptor density in moderately injured myocytes can be reversed upon removal of the injurious agent and restoration of the cellular adenosine triphosphate level ; and changes in catecholamines mediated by an intact nervous system are not required for an increase in beta - adrenergic receptor density in the setting of impaired energy metabolism . - pubmed
An acute stress , produced by crowding and confinement for 30 min , increased ACTH - IR approximately 10 - fold , and plasma cortisol levels 50 - fold , but the plasma alpha - MSH level was not affected . - pubmed
The amino acid substitution did not interfere with the synthesis , processing , and stability of the env gene polypeptides gp120 and gp41 or the binding of gp120 to its cellular receptor , the CD4 ( T4 ) molecule . - pubmed
Molecular characterization of two revertant proviral clones revealed the presence of the original mutation as well as a compensatory amino acid change in another region of HIV gp120 . - pubmed
The peak of plasma vasopressin level occurs at the same time as the late in vivo effect of aldosterone on passive 22Na efflux from arterial smooth muscle . - pubmed
Several hormones induce phosphatidylinositol turnover in cell membranes and thus activate protein kinase C. - pubmed
Utilizing in vitro microperfusion techniques , we found that activation of protein kinase C with either PMA or L - alpha-1,2 - DOG significantly inhibited net sodium absorption , net potassium secretion and transepithelial voltage in a dose - dependent manner . - pubmed
[ The HL - A system of transplantation antigens ] . - pubmed
Evidence from proton magnetic resonance data for the stacking of aromatic amino acids in lysine - vasopressin : comparison with oxytocin derivatives and related dipeptides . - pubmed
Dopamine receptor antagonism by the novel antianxiety drug , buspirone . - pubmed
The reduction of cytochrome P-450 - -CO complex in the presence of various agents in the reconstituted system of liver cell organelles was studied . - pubmed
The addition of glutamate ( but not succinate ) , NAD + and amytal ( or rotenone ) to the reconstituted system caused a 40 - 50 % reduction of NADPH - reducible cytochrome P-450 . - pubmed
The inhibitor of mitochondrial NADH - cytochrome b5 reductase dicumarol prevented the cytochrome P-450 reduction in the presence of glutamate , NAD + and amytal but did not affect the reduction of cytochrome P-450 by the added NADH . - pubmed
It was concluded that the electron transfer from the NAD - dependent substrates of the inner mitochondrial respiratory chain to the microsomal cytochrome P-450 occurs with the participation of non - bound NAD and cytochrome b5 of the outer mitochondrial membrane on the condition that the membranes of the two main oxidative systems are in tight contact . - pubmed
The effect of different antidepressants ( MAO inhibitors , monoamine uptake inhibitors , new drugs ) on the activity of bovine brain monoamine oxidase was investigated . - pubmed
The type A MAO activity was found to be selectively inhibited by the morpholine derivative , moclobamide ( IC50 - 10(-5 ) M ) . - pubmed
When injected in the presynaptic neuron , syntaxin complementary RNA also depressed Ca2 + current and transmission . - pubmed
Saturation analysis also showed that Ang II ( 0.1 nM ) increased the KD value of [ 3H ] PAC in the WKY strain but in contrast decreased the KD value of [ 3H ] PAC in the SHR . - pubmed
Hypothiocyanite production was detected in two strains and it was only detected in these strains after growth in MRS medium in aerobic conditions . - pubmed
It may be concluded that relaxations elicited by hypercapnic acidosis are associated with a fall of extracellular pH and an activation of the electrogenic Na + pump , and those caused by hyperosmolarity are due to stimulation of the Na(+)-H+ exchange and the Na+ pump . - pubmed
Discrete nuclei and other regions of the brain are solubilized in concentrated guanidine thiocyanate solution , mRNA is directly hybridized with riboprobes , and detected with a ribonuclease protection assay . - pubmed
RESULTS : N - beta - phenylethyl lidocaine was found to be a potent Na + channel blocker in vitro . - pubmed
Exceptionally stable salt bridges in cytochrome P450cam have functional roles . - pubmed
A long - standing puzzle in structure - function studies of cytochrome P450cam is how the substrate , camphor , reaches the buried active site . - pubmed
Strikingly , of the four most stable salt links in the protein ( delta Gtot & lt ; -6 kcal / mol ) , two involve the propionate groups of the heme and the other two involve Asp251 . - pubmed
Thus it appears that cytochrome P450cam has evolved four unusually strong salt bridges , stabilized by surrounding charged and polar groups in the protein , to keep its heme cofactor in place and to regulate substrate binding . - pubmed
A total of 929 lines belonging to 8 vibrational bands , 1 - 0 to 8 - 7 , for Na35 Cl , 252 lines of 1 - 0 , 2 - 1 , and 3 - 2 bands of Na37 Cl , and 355 lines of 1 - 0 , 2 - 1 , 3 - 2 , and 4 - 3 bands of K35 Cl have been measured and combined with the existing microwave and millimeter - wave data to obtain a set of refined molecular constants . - pubmed
12 - O - tetradecanoylphorbol-13 - acetate ( TPA ) binds to and activates PKC . - pubmed
Ovulation , endometrial thickness , serum oestradiol and progesterone , urinary luteinizing hormone , endometrial morphology and cervical mucus were assessed . - pubmed
Effects of poly ( ADP - ribose ) polymerase inhibition on cell death and chromosome damage induced by VP16 and bleomycin . - pubmed
We demonstrate that in lymphoblasts VP16 treatment induces apoptosis to a greater extent than BLM treatment , and that PARP inhibition reduces VP16 - induced apoptosis whereas it has no effect on BLM - induced apoptosis . - pubmed
Hence , PARP appears to play a significant role in VP16 - induced apoptosis and not in BLM - induced apoptosis . - pubmed
ap3 - 1 contains a single nucleotide change in the AP3 gene that alters both an amino acid in the AP3 protein and the 5 ' splice consensus site for intron 5 . - pubmed
PURPOSE : Brimonidine is a highly selective alpha2 - adrenoreceptor agonist that lowers intraocular pressure . - pubmed
Numerous topoisomerase I poisons including DNA minor groove binders such as Hoechst 33258 and DNA intercalators such as benzophenanthridine alkaloids and indolocarbazole derivatives have been discovered and developed . - pubmed
Many of these topoisomerase I suppressors are natural products ( beta - lapachone , diospyrin , topostatin , topostin , flavonoids ) which are believed to interact directly with the enzyme . - pubmed
Endothelial NO synthase ( eNOS ) generated NO ( 140 + /- 20 nmol l(-1 ) ) in lung during the acute phase ( first 10 - 15 min ) of endotoxemia , followed by production of NO by inducible NOS . - pubmed
In spite of cortisol replacement at 17 years , insulin requirement fell further to 0.25 U / kg / day at 18 years . - pubmed
The up - regulation of survivin expression may be due to the concurrent rise in progesterone concentrations during the normal menstrual cycle . - pubmed
Hematoxylin - eosin , Masson 's trichrome , and immunohistochemical stainings for Ulex europaeus I lectin binding , CD31 , and type IV collagen were done to differentiate lymphatic and blood vessels and to demonstrate basement membranes in vascular walls and around carcinoma cell nests was observed . - pubmed
Skeletal muscle glucose transport , plasma insulin , systolic blood pressure , and plasma lipids were measured in all groups after 22 months . - pubmed
In the HFS group , insulin - stimulated glucose transport was significantly reduced ( 67+/-4 versus 98+/-4 pmol . - pubmed
15 s(-)(1 ) ) , whereas plasma insulin ( 300+/-49 versus 82+/-8 pmol / L ) , blood pressure ( 147+/-4 versus 123+/-4 mm Hg ) , plasma triglycerides ( 2.58+/-0.31 versus 0.39+/-0.04 mmol / L ) , LDL cholesterol ( C ) ( 3.45+/-0.40 versus 0.89+/-0.06 mmol / L ) , LDL - C to HDL - C ratio ( 2.9+/-0.1 versus 2.2+/-0.1 ) , VLDL - C ( 1.53+/-0.23 versus 0.37+/-0.07 mmol / l ) , Total - C ( 5.56+/-0.58 versus 1.49+/-0.10 mmol / L ) , and body weight ( 360+/-11 versus 260+/-5 g ) were all significantly elevated compared with the LFCC . - pubmed
Conversion to a LFCC diet for 2 months led to normalization of glucose transport , blood pressure , plasma insulin , and VLDL - C and significant amelioration of obesity and other lipid abnormalities . - pubmed
Metallothionein ( MT ) is a low - molecular - weight cysteine - rich protein , which has the ability to bind and sequestrate heavy metal ions . - pubmed
Synthesis of MT is induced in a variety of tissues by these metal ions , as well as by endogenous factors such as glucocorticoids , interferon , interleukin-1 and vitamin D. - pubmed
As plasma proteins , including alpha-1 - acid glycoprotein ( AGP ) , may reduce drug efficacy through binding , AGP was investigated for its ability to interact with STI571 . - pubmed
Thus , these in vitro findings suggest that AGP will not abrogate the antileukemic activity of STI571 . - pubmed
Heparin cofactor II ( HCII ) is a plasma protein that inhibits thrombin rapidly in the presence of dermatan sulfate , heparan sulfate , or heparin . - pubmed
This alpha - LBD antibody showed high agonist activity for anti - apoptosis ( 56 + /- 9 % relative to PTN ) , low agonist activity for cell growth ( 21 + /- 1 % relative to PTN ) , and was an antagonist of PTN - induced cell growth ( 61 + /- 2 % inhibition ) . - pubmed
Specifically , we used pertussis toxin ( PTX ) as a tool to investigate the relationship between cocaine - induced alterations in cAMP signaling and behavior . - pubmed
After behavioral testing , brains were removed and processed for in vitro receptor autoradiography using the D ( 1 ) receptor ligand [ 3H ] SCH 23390 . - pubmed
These findings suggest a role for inhibitory proteins ( G ( i )/ G ( o ) ) within the nucleus acumbens in locomotor activity and also cocaine - induced behavioral sensitization . - pubmed
The benzaldehyde derivatives were more potent inhibitors of CYP2A5 than the phenylethylamines . - pubmed
The K(ic ) value of 4 - methylbenzaldehyde was 3.4 micro M and for 4 - methoxybenzaldehyde it was 0.86 micro M for CYP2A5 . - pubmed
Amphetamine is a weak inhibitor of CYP2A6 , whereas benzaldehyde is a suicide inhibitor with K(inact ) = 0.16 min(-1 ) and K(I ) = 18 micro M. The K(ic ) values of 2 - phenylethylamine , 2 -( p - tolyl )- ethylamine , 4 - methylbenzaldehyde and 4 - methoxybenzaldehyde were 1.13 , 0.23 , 0.36 and 0.73 micro M for CYP2A6 , respectively . - pubmed
Novel potent inhibitors were found for CYP2A6 and , except for 4 - methoxybenzaldehyde , all the compounds inhibited CYP2A5 and CYP2A6 enzymes differentially . - pubmed
AIP1 binds to the C - terminal domain of ASK1 via a lysine - rich cluster within the N - terminal C2 domain . - pubmed
STUDIES ON GLYCYRRHIZIN , AN ACTIVE PRINCIPLE OF RADIX LIQUIRITINE ( II ) INHIBITION BY GLUCOSACCHAROLACTONE OF DETOXYCATING ACTION OF GLYCYRRHIZIN . - pubmed
THYROXINE - BINDING BY THE THROXINE - BINDING GLOBULIN AS A MEASURE OF THE ANABOLIC ACTIVITY OF CONGENERS OF TESTOSTERONE . - pubmed
These data provide genetic evidence for the existence of tonically active MOR and DOR systems that modulate basal DA neurotransmission in the NAc . - pubmed
Furthermore , they demonstrate that in contrast to the locomotor - activating effects of morphine , a small component of morphine - evoked DA release occurs independently of MOR activation . - pubmed
Using the Chirobiotic T ( teicoplanin ) chiral stationary phase , native and derivatized theanine enantiomers were separated and detected via high - performance liquid chromatography ( HPLC ) coupled to atmospheric pressure ionization mass spectrometry ( API - MS ) . - pubmed
Optimum sensitivity and detection limits for derivatized theanine standards were achieved using ESI - MS . - pubmed
The effective doses of ICRP74 were higher than those of KORMAN with percent differences ranging from 6 % ( LLAT , 10 MeV ) to 30 % ( PA , 0.05 MeV ) . - pubmed
AEA binds to the cannabinoid receptor CB1 , located at the central nervous system , while OEA is an endogenous ligand for the alpha subtype of peroxisome - proliferator activating receptor ( PPARalpha ) . - pubmed
Besides typical central effects of cannabinoids , CB1 receptor activation leads to hyperphagia , whereas its pharmacological blockade is followed by changes in energy metabolism favouring substrate oxidation . - pubmed
OEA has inhibitory effects on food intake by acting on PPARalpha receptors which modulate the autonomous nervous system . - pubmed
Peptides obtained by proteolysis of the purified enzyme were isolated , sequenced and used to prepare degenerate oligonucleotide primers . - pubmed
The predicted amino acid sequence contains type II transmembrane region , three potential N - glycosylation sites as well as all of the isolated peptide sequences . - pubmed
The Vmax of the recombinant enzyme , 2.08 micromol/(min mg ) , was nearly the same as the native enzyme , 2.12 micromol/(min mg ) , when assayed with partially deglycosylated mucins as glycosyl acceptors . - pubmed
These results show for the first time that multiple isoforms of GalNAc transferases acting in sequence may be required for the complete O - glycosylation of mucin polypeptide chains , and those acting on the nacent polypeptide chain synthesize intermediates which can serve as glycosyl acceptors for other isoforms of the enzyme . - pubmed
EPR studies of chlorocatechol 1,2 - dioxygenase : evidences of iron reduction during catalysis and of the binding of amphipatic molecules . - pubmed
We determined the zero - field splitting of the Fe ( III ) cofactor ( D = 1.3 + /- 0.2 cm(-1 ) ) by electron paramagnetic resonance ( EPR ) experiments that along with other structural data allowed us to infer the Fe ( III ) coordination environment . - pubmed
Protein assays show that the enzyme activity is significantly lowered in the presence of stearic - acid molecules . - pubmed
Crystal structures of all five compounds show short intramolecular asymmetric hydrogen bonds ( SHB ) between adjacent carboxyl groups with O ... O distance average of 2.40 A. - pubmed
The vibrational modes , mainly SHB modes , are tentatively assigned by molecular orbital ab initio calculations of pyromellitic acid and anions [ C6H2 ( COO ) 4H3 ]- and [ C6H2 ( COO ) 4H2 ] 2 - . - pubmed
The detected ERBB2 mutation showed G to C transversion at bp 2305 ( 2305G>C ) , which would result in the substitution of Asp to His at codon 769 ( D769H ) . - pubmed
The amino acid D769 is located in the alpha - helix within the kinase domain , which is important in the binding of ATP with ERBB2 . - pubmed
In addition , we detected considerable NF2 promoter activity in various NF2 - affected tissues such as acoustic ganglion , trigeminal ganglion , spinal ganglia , optic chiasma , the ependymal cell - containing tela choroidea , and the pigmented epithelium of the retina . - pubmed
This article describes recent developments in sex - related differences in opioid ( morphine ) pharmacodynamics , morphine metabolites , the nociceptin / orphanin FQ receptor system , acute opioid tolerance and opioid - induced side - effects , such as opioid - induced respiratory depression and itch , and P - glycoprotein modulation of opioid effect . - pubmed
The structure , texture , and electronic properties of nanocomposites were studied using various characterization techniques such as N2 physisorption , X - ray diffraction ( XRD ) , laser Raman spectroscopy ( LRS ) , temperature - programmed reduction ( TPR ) , transmission electron microscopy ( TEM ) , and X - ray photoelectron spectroscopy ( XPS ) . - pubmed
TEM images reveal that the pores of SBA-15 are progressively blocked with MnO ( x ) nanoparticles . - pubmed
The surface structures of MnO ( x ) were also determined by LRS , XPS , and TPR . - pubmed
Finally , the micropatterned FAS - SAM was immersed in a solution of 1 vol % ( aminoethylaminomethyl ) phenethyltrimethoxysilane ( AEAMPS ) in absolute toluene . - pubmed
As a control , the micropatterned hydrophobic FAS - SAM template ( composed of CF3- and OH - terminated surfaces ) was also treated under the same conditions . - pubmed
Optical microscopy and atomic force microscopy confirmed that the formation of the continuous mesocomposite film proceeded only on the FAS - SAM - covered regions , while the AEAMPS - SAM - covered regions remained free of deposits . - pubmed
SCA - alpha2 , a close mimic of the metabolite , NCSi - gb , of the DNA bulge - specific enediyne antibiotic neocarzinostatin , differs in possessing a more stable spirocyclic ring system and in lacking certain bulky groupings that compromise bulged DNA binding . - pubmed
Like NCSi - gb , SCA - alpha2 possesses the natural N - methylfuranose moiety , alpha - linked to the benzindanol ( BI ) moiety . - pubmed
Lacking the bulky cyclocarbonate of NCSi - gb , the SCA-alpha2.bulged DNA complex has a much less twisted and buckled 3'-bulge - flanking base pair ( dG20.dC8 ) , and the G20 residue stacks directly above the BI ring platform . - pubmed
G - CSF , prophylactic antibiotics , and mesna were provided . - pubmed
Hemoglobin , red blood cell count , hematocrit , serum vitamin A , urinary iodine and prevalence of angular stomatitis were measured at baseline and at the end of the study after one year . - pubmed
RESULTS : There was a significant ( p<0.05 ) improvement in the experimental group in hemoglobin , red cell count , urinary iodine and serum vitamin A whereas in the control group there was a statistically significant decline ( p<0.05 ) in hemoglobin , hematocrit , red cell count and urinary iodine . - pubmed
Furthermore , experiments using the glutamatergic antagonist CNQX indicated that GA probably binds to non - NMDA receptors . - pubmed
We also provided some evidence that 3 - OHGA competes with the glutamate transporter inhibitor L - trans - pyrrolidine-2,4 - dicarboxylate , suggesting a possible interaction of 3 - OHGA with glutamate transporters on synaptic membranes . - pubmed
( 3 ) These results indicate that glutamate binding to receptors and transporters can be inhibited by GA and 3 - OHGA in cerebral cortex and striatum in a developmentally regulated manner . - pubmed
smegmatis mutants of the lipoprotein - encoding lpqW gene have a profound defect in LAM biosynthesis . - pubmed
Here we show that restoration of LAM biosynthesis in the lpqW mutant results from secondary mutations in the pimE gene . - pubmed
Our findings provide the first strong evidence that LpqW is required to channel intermediates such as AcPIM4 into LAM synthesis and that loss of PimE function results in the accumulation of AcPIM4 , bypassing the need for LpqW. - pubmed
Polarization of natural killer T cells towards an NKT2 subpopulation occurs after stimulation with alpha - galactosylceramide and rhG - CSF in aplastic anemia . - pubmed
In summary , our results indicate that polarization of NKT cells towards the NKT2 subpopulation occurs after co - stimulation with alpha - galactosylceramide and rhG - CSF in AA . - pubmed
The malondialdehyde ( MDA ) levels and activities of superoxide dismutase ( SOD ) and catalase ( CAT ) were measured and the number of apoptotic neurons was counted . - pubmed
diminuta did not affect the degree of gastric ulceration at either 3 or 7 days post - acetic acid application , as gauged by ulcer area or histopathology . - pubmed
Both kinases are key drivers of osteoclast maturation , and three agents that block osteoclastogenesis , the Src inhibitor dasatinib , the bisphosphonate alendronate , and the osteoclast - specific apoptosis - inducer reveromycin A , markedly reduced 17 - AAG - stimulated tumor growth in bone . - pubmed
Resistance to ESAs : A hyporesponse to ESAs is considered to be present when an Hb level of 11 g / dl is not achieved with a dose of epoetin & gt ; 300 IU / kg / week or a dose of darbepoetin alpha & gt ; 1.5 microg / kg / week ( Strength of Recommendation B ) . - pubmed
The L - channel agonist Bay K8644 increased the magnitude of both step and tail currents , but surprisingly failed to slow deactivation of TS channels . - pubmed
Roscovitine , a tri - substituted purine that enhances L - channel OSvdI , restored TS - impaired OSvdI. - pubmed
The cell surface intelectin-1 was not a glycosylphosphatidylinositol - anchored membrane protein . - pubmed
We demonstrate that exposure of the skin to calcipotriol before transcutaneous immunization with OVA protein and CpG adjuvant prevents Ag - specific CD8(+ ) T cell priming coincident with Langerhans cell depletion in the skin . - pubmed
Immunization through calcipotriol - treated skin induces CD4(+)CD25(+ ) regulatory T cells ( Treg ) that prevent subsequent Ag - specific CD8(+ ) T cell proliferation and IFN - gamma production . - pubmed
Topical calcipotriol treatment also induces RANKL ( receptor activator of NF - kappaB ligand ) expression by keratinocytes , a TNF family member involved in modulation of skin dendritic cells . - pubmed
The in vivo expansion of Ag - specific Treg with the topical application of the vitamin D analog calcipotriol followed by transcutaneous immunization is a simple method to augment functional Ag - specific CD4(+)CD25(+)Foxp3(+ ) Treg populations and mimics Ag - specific UV - induced tolerance . - pubmed
Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases . - pubmed
Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering , suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry , biology , and medicine . - pubmed
The relation of serum parathyroid hormone and serum calcium to serum levels of prostate - specific antigen : a population - based study . - pubmed
Experimental and clinical data implicate calcium and parathyroid hormone ( PTH ) in the development of prostate cancer . - pubmed
We examined the relationship between serum levels of calcium , PTH , and prostate - specific antigen ( PSA ) , an established marker of prostate growth , in a large , population - based study using multivariate linear regression . - pubmed
Adjusted for age , race , body mass index , and serum levels of 25 - hydroxyvitamin D , serum levels of PTH were significantly positively correlated with serum PSA ( P = 0.01 ) . - pubmed
Serum levels of PTH and calcium each were correlated significantly with free PSA ( P = 0.05 and 0.008 , respectively ) . - pubmed
These findings support the hypothesis that serum calcium and serum PTH stimulate prostate growth in men without clinical prostate cancer and have implications for the use of PSA as a screening tool for prostate cancer . - pubmed
Comparative 16S rRNA gene stable isotope probing ( SIP ) in the presence and absence of sulfate indicated that a Desulfosporosinus species , which constitutes only 0.006 % of the total microbial community 16S rRNA genes , is an important sulfate reducer in a long - term experimental peatland field site . - pubmed
Parallel SIP using dsrAB ( encoding subunit A and B of the dissimilatory ( bi ) sulfite reductase ) identified no additional sulfate reducers under the conditions tested . - pubmed
The link of DAP1 to autophagy was first apparent in that its knockdown enhanced autophagic flux and in that it displayed a rapid decline in its phosphorylation in response to amino acid starvation . - pubmed
Mapping of the phosphorylation sites and analysis of phosphorylation mutants indicated that DAP1 is functionally silenced in growing cells through mTOR - dependent phosphorylations on Ser3 and Ser51 . - pubmed
We found that exposure to genistein did not affect circulating levels of the androgen 11 - ketotestosterone or the estrogen E2 relative to negative - control fish . - pubmed
Caveolin 1 is required for the activation of endothelial nitric oxide synthase in response to 17beta - estradiol . - pubmed
Evidence suggests that estrogen mediates rapid endothelial nitric oxide synthase ( eNOS ) activation via estrogen receptor - a ( ERalpha ) within the plasma membrane of endothelial cells ( EC ) . - pubmed
However , the role played by caveolin 1 in regulating 17beta - estradiol - mediated NO signaling in EC has not been adequately resolved . - pubmed
Thus , the purpose of this study was to explore how 17beta - estradiol stimulates eNOS activity and the role of caveolin 1 in this process . - pubmed
Further , before estrogen stimulation ERalpha associates with caveolin 1 , whereas stimulation promotes a pp60(Src)-mediated phosphorylation of caveolin 1 at tyrosine 14 , increasing ERalpha - PI3 kinase interactions and disrupting caveolin 1 - ERalpha interactions . - pubmed
Furthermore , Y14FCav overexpression reduced eNOS phosphorylation at serine1177 and decreased NO generation after estrogen exposure . - pubmed
Delivery of a synthetic peptide based on this sequence decreased ERalpha plasma membrane translocation and reduced estrogen - mediated activation of eNOS . - pubmed
In conclusion , caveolin 1 stimulates 17beta - estradiol - induced NO production by promoting ERalpha to the plasma membrane , which facilitates the activation of the PI3 kinase pathway , leading to eNOS activation and NO generation . - pubmed
The catalytic reduction of NO ( 2 ) with hydrogen on a Pt field emitter tip is investigated using both field electron microscopy ( FEM ) and field ion microscopy ( FIM ) . - pubmed
One - pot conversions of olefins to cyclic carbonates and secondary allylic and homoallylic amines to cyclic carbamates . - pubmed
Sequential treatment of a 1,2 - disubstituted olefin with m - CPBA , Br3CCO2H , and DBU results in the one - pot , stereospecific conversion of the olefin to the corresponding disubstituted cyclic carbonate ( 1,3 - dioxolan-2 - one ) . - pubmed
Tyrosinase is the key and rate - limiting enzyme responsible for the conversion of tyrosine into melanin . - pubmed
DeoxyArbutin not only shows greater inhibition of tyrosinase activity but is also safer than hydroquinone and arbutin . - pubmed
The expression of mtc was induced after exposure to all metals , except for one chromium concentration . - pubmed
Exposure to soil originating from metal - contaminated field sites also induced mtc , while the expression did not change in response to a polycyclic aromatic hydrocarbon . - pubmed
Furthermore , we determined the effects of increasing extracellular K (+ ) concentrations ( [ K(+)](o ) ) and inhibition of Na (+) , K (+)- ATPase on neurogenic and norepinephrine - induced contractile responses . - pubmed
The neuromodulatory effects of PGEs were tested in the presence of K (+ ) channel blockers . - pubmed
The results demonstrate that PGE ( 1 ) and PGE ( 2 ) inhibit adrenergic responses by a prejunctional mechanism that involves the activation of large - conductance Ca ( 2 +)- activated K (+ ) channels and Na (+) , K (+)- ATPase . - pubmed
Leucine - rich amelogenin peptides regulate mineralization in vitro . - pubmed
Amelogenin 's capacity to regulate enamel formation is related to its conserved N- and C - terminal domains , its ability to self - assemble , and its ability to stabilize amorphous calcium phosphate ( ACP ) - a capacity enhanced by amelogenin phosphorylation . - pubmed
TEM , selected area electron diffraction , and Fourier transform - infrared spectroscopy were also used to determine the effect of phosphorylated and non - phosphorylated LRAP on calcium phosphate formation . - pubmed
Notably , this capacity was enhanced by added calcium and to a much greater degree for phosphorylated LRAP . - pubmed
Furthermore , phosphorylated LRAP was found to stabilize ACP and prevent its transformation to hydroxyapatite ( HA ) , while aligned HA crystals formed in the presence of non - phosphorylated LRAP . - pubmed
The N- and C - terminal amelogenin domains in non - phosphorylated LRAP are , therefore , sufficient to guide ACP transformation into ordered bundles of apatite crystals , making LRAP an excellent candidate for biomimetic approaches for enamel regeneration . - pubmed
Sandblasting abrasion with 50 micro alumina particles at a specific distance , pressure and time was the only surface treatment in DT Light Post and Transluma Post that increased the bond strength to dual cure resin composite cores . - pubmed
We have previously shown that amphiregulin ( AREG ) , a ligand to the epidermal growth factor receptor ( EGFR ) , is induced during the repair / differentiation process elicited by naphthalene - induced lung injury . - pubmed
We show that AREG and epithelial EGFR expression is dispensable to pulmonary epithelial repair after naphthalene - induced lung injury , but regulates secretory cell differentiation to a mucus - producing phenotype . - pubmed
We show that the pulmonary epithelium is the source of AREG , suggesting that naphthalene - induced MCM is mediated through an autocrine signaling mechanism . - pubmed
Our data demonstrate that AREG - dependent EGFR signaling in airway epithelial cells contributes to MCM in naphthalene - induced lung injury . - pubmed
Analyses using MTT assay and measurement of lactate dehydrogenase release showed that presence of 27 - hydroxycholesterol counteracted the toxic effects of staurosporine on these cells . - pubmed
Also , 27 - hydroxycholesterol significantly decreased the staurosporine - mediated induction of caspase-3 and - 7 , known to be important in apoptotic events . - pubmed
Therefore , the molecular signaling events affecting resveratrol - mediated heme oxygenase-1 ( HO-1 ) and glyoxalase expression levels were further investigated in this study . - pubmed
Our findings indicated that resveratrol activated the extracellular signal - regulated kinase ( ERK ) pathway but not the p38 or c - Jun N - terminal kinase ( JNK ) pathways , subsequently leading to Nrf2 nuclear translocation and elevation of HO-1 and glyoxalase expression levels . - pubmed
The efficacy of agents that alkylate the O-6 position of guanine is inhibited by O ( 6 )- alkylguanine - DNA alkyltransferase ( AGT ) which removes these lesions from the tumor DNA . - pubmed
A newly synthesized prodrug of the AGT inhibitor O ( 6 )- benzylguanine ( O ( 6 )- BG ) with an alpha , alpha - dimethyl-4 - nitrobenzyloxycarbonyl moiety masking the essential 2 - amino group has demonstrated the feasibility of targeting hypoxic regions that are unique to solid tumors , for drug delivery . - pubmed
Recently , new potent global AGT inhibitors with improved formulatability such as O ( 6 )-[( 3 - aminomethyl ) benzylguanine ( 1 ) have been developed . - pubmed
The findings show a significant modulation effect of cadmium on H - ras gene expression accompanied by up - regulation of caspase-3 - related apoptosis in the concentration range of 100 - 1000 nMu cadmium . - pubmed
Consequently , the blood calcium , calciotropic hormones , and skeleton are normal at birth despite severe vitamin D deficiency or genetic deletion of calcitriol or vitamin D receptor . - pubmed
This study compares the in vitro and in vivo properties of ASD material generated by fast evaporation , lyophilization , and spray - drying methods using griseofulvin as a model compound and hydroxypropyl methylcellulose acetate succinate as the polymer matrix . - pubmed
Lesatropane , a synthesized chiral tropane ( 3S , 6S - isomer of satropane ) , is a novel muscarinic agonist , and is being under preclinical development in China for the treatment of primary glaucoma . - pubmed
The reports concerning that activation of muscarinic acetylcholine receptors ( mAChRs ) could protect cells against apoptosis prompted us to study the neuroprotective effects of lesatropane and the mechanism . - pubmed
Atropine or pirenzepine , antagonist of mAChR or M1 mAChR , antagonized the protective effects of lesatropane respectively and suppressed the lesatropane 's effects on ERK1 / 2 . - pubmed
Furthermore , chelerythrine , a PKC inhibitor , partially suppressed ERK1 / 2 activation induced by lesatropane . - pubmed
The results indicated that the specific M1 mAChR via PKC - ERK1 / 2 pathway might be involved in the neuroprotective effects of lesatropane . - pubmed
Palmitic acid induces production of proinflammatory cytokines interleukin-6 , interleukin-1beta , and tumor necrosis factor - alpha via a NF - kappaB - dependent mechanism in HaCaT keratinocytes . - pubmed
Pyrrolidine dithiocarbamate ( PDTC , a selective chemical inhibitor of NF - kappa B ) and goat anti - human IL-6 polyclonal neutralizing antibody were used to inhibit NF - kappa B activation and IL-6 production , respectively . - pubmed
Our results showed that palmitic acid induced an upregulation of IL-6 , TNF - alpha , IL-1 beta secretions , accompanied by NF - kappa B nuclear translocation and activation . - pubmed
Moreover , the effect of palmitic acid was accompanied by PPAR alpha activation and Stat3 phosphorylation . - pubmed
Palmitic acid - induced IL-6 , TNF - alpha , IL-1 beta productions were attenuated by NF - kappa B inhibitor PDTC . - pubmed
Palmitic acid was administered in amounts able to elicit significant hyperproliferation and can be attenuated by IL-6 blockage . - pubmed
These data demonstrate for the first time that palmitic acid can stimulate IL-6 , TNF - alpha , IL-1 beta productions in HaCaT keratinocytes and cell proliferation , thereby potentially contributing to acne inflammation and pilosebaceous duct hyperkeratinization . - pubmed
In ovo administration of high dose CORT significantly suppressed the growth rate from 3weeks of age and increased the frequency of aggressive behaviors , and the dosage was associated with elevated plasma CORT concentrations and significantly downregulated hypothalamic expression of arginine vasotocin ( AVT ) and corticotropin - releasing hormone ( CRH ) . - pubmed
High dose CORT exposure significantly increased platelet serotonin ( 5 - HT ) uptake , decreased whole blood 5 - HT concentration ( p<0.05 ) , downregulated hypothalamic tryptophan hydroxylase 1 ( TPH1 ) mRNA and upregulated 5 - HT receptor 1A ( 5 - HTR1A ) and monoamine oxidase A ( MAO - A ) mRNA , but not monoamine oxidase B ( MAO - B ) . - pubmed
High dose CORT also significantly increased DNA methylation of the hypothalamic GR and CRH gene promoters ( p<0.05 ) . - pubmed
In marginal structural models , IL-1beta , IL-6 , IL-8 were associated with lower estradiol and progesterone concentrations . - pubmed
Urushiol induced cytostatic cell growth inhibition via upregulation of the cyclin - dependent kinase inhibitors , p21 ( WAF1 / CIP1 ) and p27 ( KIP1 ) proteins and down - regulation of cyclin - dependent kinase 2 and 4 proteins in a p53 - independent manner . - pubmed
DNA polymerases engineered by directed evolution to incorporate non - standard nucleotides . - pubmed
DNA polymerases have evolved for billions of years to accept natural nucleoside triphosphate substrates with high fidelity and to exclude closely related structures , such as the analogous ribonucleoside triphosphates . - pubmed
However , polymerases that can accept unnatural nucleoside triphosphates are desired for many applications in biotechnology . - pubmed
This review focuses on experiments that , by directed evolution , have created variants of DNA polymerases that are better able to accept unnatural nucleotides . - pubmed
After a therapeutic lefetamine dose , the bis - nor , bis - nor - hydroxy , nor - hydroxy , nor - di - hydroxy metabolites could be detected using the authors ' GC - MS SUSA and the nor - hydroxy - glucuronide by the LC - MS(n ) SUSA . - pubmed
In immature neurons , GABA ( A ) R activation leads to neuronal depolarization and activation of Ca ( 2 + ) signals , which mediate many of the developmental effects of GABA . - pubmed
BRD4 promotes pancreatic ductal adenocarcinoma cell proliferation and enhances gemcitabine resistance . - pubmed
Furthermore , we showed that the expression of BRD4 was increased after treatment with gemcitabine ( GEM ) . - pubmed
Here we report a theoretical study on the binding of two molecules , 05B and P4O , to the proteins MK2 and MK3 . - pubmed
Acid - resistant proteins , such as mucins , were identified after pellicle was exposed to lactic or citric acid . - pubmed
Further comparison of the MCI and NC groups revealed that declarative memory was inversely associated with cortisol levels ( P & lt ; 0.001 ) , but this association became marginal when 25 - hydroxy vitamin D was included in the linear multivariate regression analyses ( P = 0.06 ) . - pubmed
The cognitive impairment may be the result of HPA axis dysregulation but 25 - hydroxy vitamin D serum concentrations might be compensatory or even a potent contributing factor . - pubmed
Cell cycle was changed in the transformed cells after EGCG treatment with significantly increased cell numbers in G0 / G1 phase and decreased cell numbers in S phase compared to control group , P & lt ; 0.001 . - pubmed
Both mRNA and protein levels of hTERT gene were significantly decreased in cells after treated with EGCG , P & lt ; 0.001 . - pubmed
c - Myc protein level was significantly decreased after EGCG treatment , especially in the highest dose group ( i.e. - pubmed
A silver island film ( SIF ) substrate was used to demonstrate that Metal - Enhanced Fluorescence ( MEF ) is a powerful tool to enable detection of emission from ( bio)molecules at very low concentrations . - pubmed
The VEGF concentrations of combination group [ ( 94.61 + - 5.46 ) ng / L decreased significantly , as compared to thalidomide group [ ( 141.11 + - 3.70 ) ng / L and IFN group [ ( 119.90 + - 2.00 ) ng / L ( P & lt ; 0.05 ) . - pubmed
Stabilizing hypoxia inducible factor-1alpha with dimethyloxallyl glycine increased early survival , but not proliferation or differentiation , in neurospheres in vitro and in newly born SGZ cells in vivo . - pubmed
Microbial phytase - catalyzed phytate dephosphorylation was accompanied by increased iron and zinc release from the cereal substrates . - pubmed
No increases in soluble cadmium , lead or arsenic were observed with microbial phytase - catalyzed phytate dephosphorylation . - pubmed
CONCLUSION : Microbial phytase treatment abated phytate chelation hence enhanced the release of iron and zinc from phytate - rich cereals under simulated gastric conditions . - pubmed
The data infer that acid - stable microbial phytases can help improve iron bioavailability from phytate - rich cereal substrates via post - ingestion activity . - pubmed
Uniform rhodium nanoparticles ( NP ) with three different particle sizes ( 1.9 , 2.4 , and 3.6 nm ) were prepared via a polyol method with rhodium ( III ) acetylacetonate , poly ( vinylpyrrolidone ) with different concentrations of sodium citrate . - pubmed
The recombinant virus DC474 - 480 constructed with tyrosines 474 , 476 , 477 , and 480 mutated to alanine residues produced a gK - null - like phenotype characterized by the production of very small plaques and accumulation of capsids in the cytoplasm of infected cells . - pubmed
Immunoprecipitation assays revealed that replacement of all four tyrosines with alanines substantially reduced the ability of gK to interact with UL37 . - pubmed
Collectively , these results suggest that the UL37 conserved tyrosine 480 residue plays a crucial role in interactions with gK to facilitate cytoplasmic virion envelopment and infectious virus production . - pubmed
We show here that UL37 tyrosine residues conserved among all alphaherpesviruses serve critical roles in cytoplasmic virion envelopment and interactions with gK. - pubmed
We propose a method to specifically study the impact of actomyosin contractility on the intracellular dynamic signal by performing high throughput , comparative measurements of multicellular aggregates with and without blebbistatin action , a selective inhibitor of class - II myosins that disrupts actomyosin contractile activity . - pubmed
A three - dimensional hemin - functionalized graphene hydrogel ( Hem / GH ) was prepared by a facile self - assembly approach . - pubmed
The as - prepared Hem / GH showed good mechanical strength with a storage modulus of 609 - 642 kPa and a high adsorption capacity to organic dye contaminants ( 341 mg g-1 for rhodamine B ) . - pubmed
Moreover , Hem / GH could be used as a photosensitizer for the photocatalytic degradation of organic dyes and displayed superior photodegradation activity of methylene blue ( MB ) . - pubmed
This result was better than that of counterparts such as graphene hydrogel ( GH ) and commercial catalyst P25 . - pubmed
The excellent cycling performance of the Hem / GH was well maintained even after multiple cycles on adsorption process and photocatalytic reaction . - pubmed
As an extended application , visually intuitive observation showed the as - prepared Hem / GH also had strong antibacterial properties . - pubmed
Cellular mechanisms contributing to the complex machinery executing eryptosis include increase of cytosolic Ca2 + activity ( [ Ca2+]i ) , oxidative stress , ceramide , protein kinase C and caspases . - pubmed
METHODS : Phosphatidylserine exposure at the cell surface was estimated from annexin - V - binding , cell volume from forward scatter , [ Ca2 +]i from Fluo-3 fluorescence , ROS formation from DCFDA dependent fluorescence , and ceramide abundance utilizing specific antibodies . - pubmed
The effect of Camalexin on annexin - V - binding was significantly blunted by removal of extracellular Ca2 + , by kinase inhibitors staurosporine ( 1 microM ) and chelerythrine ( 10 microM ) , as well as by caspase inhibitors zVAD ( 10 microM ) and zIETD - fmk ( 50 microM ) . - pubmed
CONCLUSIONS : Camalexin triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane , an effect at least in part depending on Ca2 + entry , as well as staurosporine and chelerythrine sensitive kinase(s ) as well as zVAD and zIETD - fmk sensitive caspase ( s ) . - pubmed
Fifty - six maxillaryanterior - teeth were filled with TAP for 28 days , and assigned into 5 experimental groups ( n=10 ): Syringe irrigation ( SI ) , NaviTip FX ( NFX ) , Vibringe - Syringe irrigation ( V - SI ) , NaviTip FX - Vibringe ( V - NFX ) , passive ultrasonic irrigation ( PUI ) and positive and negative control groups ( n=3 for each ) . - pubmed
V - NFX and NFX showed similar performances ( p>0.05 ) while V - NFX was significantly more efficient than SI and PUI ( p<0.05 ) at all parts of root canals . - pubmed
VSI was less efficient at apical part when compared to NFX and V - NFX ( p<0.05 ) while not significantly different from PUI and SI at all parts of the root canals ( p>0.05 ) . - pubmed
The median sphenoid sinus mucosal thickness was greater in the PA than NFPA groups ( 2.0 vs. 0.5 mm ; p & lt ; 0.001 ) . - pubmed
The small , stable heme protein myoglobin ( Mb ) was modified through cofactor substitution and mutagenesis to develop a new catalyst for carbene transfer reactions . - pubmed
The native heme was removed from wild - type Mb and several Mb His64 mutants ( H64D , H64A , H64V ) , and the resulting apoproteins were reconstituted with ruthenium mesoporphyrin IX ( RuMpIX ) . - pubmed
Sperm counts or motility was significantly reduced by BPA , BPE or BPS exposure on PND 60 or PND 90 . - pubmed
PCSK9 inhibitors , apolipoprotein ( a)-antisense , and apolipoprotein ( B-100)-antisense mipomersen have shown promising results . - pubmed
Over 4 years he had recurrent disease in the face and liver and was treated with nab - paclitaxel , surgery , and radioembolization , but continued to have progressive disease . - pubmed
His tumor was found to express PD - L1 and he received off - label pembrolizumab 2 mg / kg every 21 days for 13 cycles with marked shrinkage of his liver disease and no new facial lesions . - pubmed
The HCB increased with increasing competitive interactions described by tree - centered competition measure : BAL or HCI , and species mixing effects described by BAPOR . - pubmed
In addition , FPHPe1 significantly ( p & lt ; 0.05 ) reduced SBP ( maximum -33 mmHg ) , DBP ( maximum -24 mmHg ) , MAP ( maximum -28 mmHg ) , and HR ( maximum -58 beats ) in SHRs . - pubmed
BACKGROUND : The inositol 1,4,5 - triphosphate ( IP3 ) receptor type 1 gene ( ITPR1 ) encodes the IP3 receptor type 1 ( IP3R1 ) , which modulates intracellular calcium homeostasis and signaling . - pubmed
Acetylation of alpha - tubulin at Lys-40 is a potential biomarker for cognitive deficits in various neurological disorders . - pubmed
This method has been successfully applied in a preclinical study of organophosphate neurotoxicity , and we found that chronic exposure to chlorpyrifos led to a significant and persistent inhibition of brain tubulin acetylation . - pubmed
In order to understand water chemistry after impoundment of the Three Gorges Reservoir ( TGR ) , the authors assessed the hydrogeochemical parameters ( water temperature , pH , conductivity , dissolved O2 ) , major element composition , and the carbon stable isotopic value of dissolved inorganic carbon ( delta13CDIC ) in summer and winter at various depths in the water column at TGR in 2009 . - pubmed
Ultimately , miR372 promotes the expression of erbB-2 through PKM2 - pH3T11 - acetylation on histone H3 lysine 9 ( H3K9Ac ) pathway . - pubmed
Fluorescence in situ hybridization demonstrated an ETV6 - neurotrophic tyrosine kinase , receptor , type 3 gene ( NTRK3 ) translocation but no ALK , ROS1 , or PDGFR - beta translocations . - pubmed
Materials and Methods : Thirty specimens of each denture base material : Heat cure polymethylmethacrylate ( DPI heat cure ) , high - impact polymethylmethacrylate ( Trevalon HI ) , and nylon ( Valplast ) were fabricated . - pubmed
Voclosporin , a novel calcineurin inhibitor , has been investigated as induction therapy in LN in combination with myocophenolate mofetil ( MMF ) and a glucocorticoid ( GC ) . - pubmed
Two phase II trials of voclosporin were the first trials of a potential treatment of active LN that met their primary endpoints . - pubmed
In the phase IIb randomized controlled trial AURA - LV , voclosporin was shown to be superior to placebo , when used in combination with MMF ( 1 - 2 g / day ) and GC , in achieving remission in active LN . - pubmed
Rib Fractures Mimicking Bone Metastases in 18F - PSMA-1007 PET / CT for Prostate Cancer . - pubmed
PET / CT showed F - PSMA-1007 uptake in healing rib fractures with no other pathologic findings . - pubmed
In this symposium , upcoming challenges of the modern MS techniques , such as electrospray ionization- and matrix - assisted laser desorption ionization - MS , in clinical tests for discovering biomarkers are being presented for the rapid diagnoses of infectious diseases and amyloidosis , and rapid detections of hormones and lipids in clinical samples by 4 speakers . - pubmed
Fragment - based ligand design , De novo design , ADMET and Molecular docking were performed to explore potential selective SHP2 allosteric inhibitors based on SHP836 . - pubmed
Two - pore domain K + ( K2P ) channels generate K+ leak current , which serves a vital role in controlling and modulating neuronal excitability . - pubmed
Engineering transcription factor BmoR for screening butanol overproducers . - pubmed
This study took transcription factor BmoR as an example and aimed to expand the detection range of BmoR for screening alcohols overproducers . - pubmed
Structure of BmoR - isobutanol complex were modelled , and isobutanol binding sites were confirmed by site - directed mutagenesis . - pubmed
Compared with wild - type BmoR , F276A / E627N BmoR and D333N / E627N BmoR have wider detection ranges ( 0 - 100 mM ) and relatively high outputs to the isobutanol added quantitatively or produced intracellularly , demonstrating they have potential for screening isobutanol overproduction strains . - pubmed
PAS staining indicated localization of deposits in the sub - retinal pigment epithelia ( RPE ) layer . - pubmed
The morphology and textural properties of ILM / ZIF-8 were characterized by SEM , TEM , BET and BJH . - pubmed
The ILM / ZIF-8 was applied to the concentration and determination of aflaoxins ( AFB1 , AFB2 , AFG1 and AFG2 ) in milk samples based on magnetic solid phase extraction ( MSPE ) coupled with ultra high performance liquid chromatography - tandem mass spectrometry ( UHPLC - MS / MS ) . - pubmed
ER - mediated anti - tumor effects of shikonin on breast cancer . - pubmed
This study aims to assess the effects of shikonin on the cell proliferation , cell cycle and cell apoptosis of BC and whether the effects are related to ER / GPER signaling pathway . - pubmed
Furthermore , shikonin downregulated the expression of ERalpha and GPER , and this effect was not affected by the estrogen environment . - pubmed
Albumin - stabilized manganese - based nanocomposites with sensitive tumor microenvironment responsivity and their application for efficient SiRNA delivery in brain tumors . - pubmed
Arg - Gly - Asp ( RGD ) peptide - decorated bovine serum albumin ( BSA ) was employed as the stabilizer and scaffold to fabricate Mn ( iii )- and Mn ( iv )-integrated nanocomposites ( RGD - BMnNPs ) with suitable size , good stability , and excellent biocompatibility . - pubmed
These novel albumin - stabilized manganese - based NPs combined with efficient drug delivery capacity hold great potential to serve as intelligent theranostic agents for further clinical translation . - pubmed
The asymmetric supercapacitor ( ASC ) device fabricated by employing Ni - CoP3 and activated carbon ( AC ) as positive and negative electrodes , resepectively , exhibits a remarkable high energy density of 89.6 Wh kg-1 at 796 W kg-1 and excellent stability of 93 % after 10 000 cycles , due to the skutterudite structure . - pubmed
At 24hr.after surgery , a decrease in hemoglobin in the DDAVP group was significantly lower than that in the NS group ( -5.0 + -6.9 g / L vs. -10.2 + -9.3g / L , p=0.03 ) . - pubmed
Sonoclot showed that the platelet function in the DDAVP group was higher than that in NS group at 24 hr . - pubmed
Kinetic studies were performed using 4 - nitrophenol ( 4 - NP ) as the model substrate , using the Langmuir - Hinshelwood model . - pubmed
CONCLUSIONS : Our results suggest that SP , CGRP and noradrenaline may promote , while acetylcholine may stall , the progression of adenomyosis through their respective receptors on adenomyotic lesions . - pubmed
The neoantigen migrated in immunoelectrophoresis as an alpha2 - beta globulin , had an average molecular size of 7S , and was soluble in 50 % saturated ammonium sulfate . - pubmed
Expression of K-19 was evaluated by immunoperoxidase staining in formalin - fixed normal ectocervical tissue , normal endocervical tissue , cervical dysplasia , squamous metaplasia , atrophic epithelium , cervical condylomas , and invasive carcinoma to determine if a correlation of K-19 expression with dysplasia was present in the cervical epithelium . - pubmed
Paraffin sections of brain containing the ependymal wound were reacted for glial fibrillary acidic protein by the avidin biotin complex technique . - pubmed
Non - hydrolysable analogues of GTP , such as GTP gamma S and GMP - PNP , have previously been shown to inhibit the formation of constitutive secretory vesicles ( CSVs ) and immature secretory granules ( ISGs ) from the trans - Golgi network ( TGN ) . - pubmed
ADP - ribosylation experiments revealed the presence of a pertussis toxin - sensitive G - protein alpha subunit in the TGN . - pubmed
Several treatments from the latter category , namely , hypertensive , hypervolemic hemodilutional therapy and the calcium channel blocker nimodipine , have proven moderately effective and are in widespread clinical use . - pubmed
However we are still in need of an effective agent to prevent arterial narrowing , and several hopeful candidates in this category of treatment are clot lytic agent tissue plasminogen activator ( rt - PA ) and an inhibitor of iron - dependent peroxidation , 21 - aminosteroid U74006F ( tirilazad mesylate ) . - pubmed
The most likely explanation for increased CSF lactate is ammonia - induced inhibition of malate - aspartate shuttle and/or inhibition of tricarboxylic acid cycle flux in brain . - pubmed
The ras - related rap2 gene encodes a 21 kDa GTP - binding protein that exhibits many structural similarities with Ras proteins . - pubmed
In particular , it contains a C - terminal CAAX sequence ( C , cysteine ; A , aliphatic residue ; X , any amino acid ) which has been shown to direct the post - translational modifications responsible for membrane binding of Ras proteins and nuclear lamins . - pubmed
During its maturation , the Rap2 protein is modified by the attachment of both palmitate and polyisoprenoid groups , as is also the case for H - and N - Ras proteins . - pubmed
Subcellular fractionation by sucrose density centrifugation as well as indirect immunofluorescence experiments show that the Rap2 protein is localized in a low - density compartment that morphologically overlaps with the endoplasmic reticulum , whereas Ras proteins are associated with the plasma membrane . - pubmed
17 beta - Bromoacetoxydihydrotesterone ( BA - DHT ) reacted with highly purified SHBG in a time - dependent and irreversible fashion . - pubmed
At the completion of the reaction , the molar ratio of BA - DHT to SHBG was approximately unity . - pubmed
SHBG was affinity labeled with [ 14C ] BA - DHT and submitted to acid hydrolysis . - pubmed
Furthermore , [ 14C ] BA - DHT - labeled SHBG was digested with trypsin , followed by isolation of the released tryptic peptides by reverse - phase high performance liquid chromatography . - pubmed
It contained 2 ' histidyl residues , corresponding to residues 235 and 251 in the known amino acid sequence of SHBG . - pubmed
Cancer cells were equally stained with fluorescent R 1881 and mibolerone , and this fluorescence seems to be made up of both the androgen receptor and progestin - binding protein . - pubmed
The immunoreactivities of these McAbs to 33 KD appear to be directed to peptide epitopes but not to carbohydrate moiety . - pubmed
An increase in the [ Ca2+]o from 0.45 to 3.6 mM depressed both the spontaneous and the nicotine - induced release of ACh , while the releases of ACh induced by electrical - field stimulation ( EFS ) or by high K + were augmented . - pubmed
IND and PGE2 did not modify the release of ACh induced by EFS and high K + , at any given [ Ca2+]o tested . - pubmed
Cyclic AMP perturbation of intact cells caused induction of both phosphorylase -' a ' activity and the 104 - kDa protein . - pubmed
Although a causative contribution of endogenous ANF in the natriuretic response to acute hypervolemia is well established , its participation in the maintenance of salt balance during large variations in dietary Na intake is less certain . - pubmed
In addition to increased sodium excretion , atrial factor also inhibits aldosterone and renin release , both in vitro and in vivo . - pubmed
the change in pulse interval in response to a phenylephrine - induced increase in blood pressure , was compared with ( 1 ) haemodynamic changes during mental arithmetic , a reaction time test , isometric and bicycle exercise ; ( 2 ) plasma noradrenaline ( PNA ) concentrations at rest , and during bicycle exercise and ; ( 3 ) the variability of ambulatory intra - arterial blood pressure . - pubmed
A specific anti - NAS antibody and fluorescein - labelled second antibody were employed to investigate the presence of NAS in the dentate gyrus of the hippocampus as a function of age . - pubmed
The enzyme is free of lipase activity , and possesses a very high sterospecificity on S - naproxen [ 2 -( 6 - methoxy-2 - naphthyl )- propionic acid ] esters and esters of related drugs . - pubmed
The primary structure of the enzyme , determined from the nucleotide sequence , shows limited homology with the catalytic site of lipases . - pubmed
To increase the resistance of the enzyme , lysine residues thought to be responsible for this phenomnon were replaced through site - directed mutagenesis . - pubmed
Potentiation by ACE inhibitors of the dilator response to bradykinin in the coronary microcirculation : interaction at the receptor level . - pubmed
Continuous infusion of bradykinin ( 10 nM ) elicited a transient decrease in coronary perfusion pressure ( CPP ) and increased prostacyclin ( PGI2 ) release which returned to baseline values within 30 min . - pubmed
Subsequent co - infusion of ramiprilat ( & gt ; or = 10 nM ) or moexiprilat , but not of the less potent ACE inhibitor n - octyl - ramipril ( RA - octyl ) , caused another fall in CPP and an increase in PGI2 release , the magnitude and time course of which were almost identical to the first response to bradykinin . - pubmed
No change in CPP or PGI2 release was observed when the ACE inhibitors were administered in the absence of exogenous bradykinin . - pubmed
Infusion of D - Arg [ Hyp3 ]- bradykinin ( 10 nM ) , a specific B2 - receptor agonist which was significantly more resistant to degradation by ACE than bradykinin , produced virtually identical changes in CPP and PGI2 release when compared to bradykinin . - pubmed
Subsequent co - infusion of ramiprilat was similarly effective in restoring the fall in CPP and increase in PGI2 release elicited by D - Arg [ Hyp3 ]- bradykinin as in the presence of bradykinin . - pubmed
In concentrations which should block the degradation of bradykinin by ACE in the coronary vascular bed , two ACE substrates , hippuryl - L - histidyl - L - leucine ( 0.2 mM ) and angiotensin I ( 0.3 microM ) , were unable to elicit a significant change in CPP or PGI2 release while ramiprilat and another ACE inhibitor , quinaprilat , were still active in the presence of these substrates . - pubmed
Ramiprilat ( 0.1 MicroM ) , but not RA - octyl ( 1 MicroM),potentiated the endothelium - independent , B2 - receptor - mediated constrictor response to bradykinin , but not that to the thromboxane - mimetic U46619 ( 9,11 - dideoxy - ll alpha,9 alpha - epoxymethano - prostaglandin F2.).Moreover , ramiprilat but not RA - octyl caused a concentration - dependent , B2 - receptor antagonist sensitive increase in tone when administered alone.7 . - pubmed
Bilateral simultaneous inferior petrosal sinus corticotropin sampling with corticotropin - releasing hormone stimulation was performed before transphenoidal pituitary adenomectomy , with successful localization of the pituitary adenoma . - pubmed
The test relies on the capacity of the kidney to retain H + during gastric acid secretion , leading to a post - prandial urine ' alkaline tide ' . - pubmed
Different qualitative patterns are seen when the activities of alkaline phosphatase ( AP ) , invertase ( INV ) , or [ 3H ] methylthymidine incorporation are expressed on the basis of millimoles per gram protein , percentage of highest activity , percentage of total activity , cumulative activity for each fraction , or millimoles per gram protein versus cumulative percentage of total protein . - pubmed
To identify gene products that interact with Notch , a second - site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated Notch molecules . - pubmed
Metallothioneins ( MTs ) are low molecular weight and cysteine - rich intracellular proteins involved in metal homeostasis and detoxication . - pubmed
nidulans imply a role for topoisomerases in amplification and replication of circular DNA molecules . - pubmed
Tubulin is a very water soluble protein , yet a significant portion is firmly associated with cell membranes . - pubmed
More palmitate of the pellet was found in alpha than beta tubulin ; EGF stimulation primarily increased palmitate in beta tubulin . - pubmed
In addition , blood samples were taken at rest and at steady state during exercise , and plasma levels of vasopressin and renin activity were assessed . - pubmed
At rest and during all levels of exercise , muscarinic cholinergic receptor blockade caused a marked increase in HR over control ( saline treated ) levels with little effect on MAP or TAQ . - pubmed
Summary of the nephron distribution of PDE activities showed that cAMP - PDE activities were detected in all nephron segments and the distal convoluted tubules ( DCTs ) had the highest activity . - pubmed
Both in proximal convoluted tubules ( PCTs ) and medullary collecting ducts ( MCDs ) , more than 80 % of the total cAMP - PDE activities were inhibited by a nonspecific PDE inhibitor , 3 - isobutyl-1 - methylxanthine ( IBMX ) . - pubmed
In PCTs , rolipram ( type - IV PDE inhibitor ) was an equally potent inhibitor of IBMX , while 8 - methoxymethyl - IBMX ( MM - IBMX , type - I PDE inhibitor ) and cilostamide ( type - III PDE inhibitor ) inhibited cAMP - PDE by 35 and 57 % , respectively . - pubmed
Rolipram potentiated the parathyroid hormone ( PTH)-induced cAMP content in PCTs most effectively . - pubmed
Cilostamide had no effect on the cAMP content stimulated by PTH or AVP in PCTs and MCDs . - pubmed
Among these , type - IV PDE was responsible for hydrolysis of cAMP in PCTs , and type - I and IV PDE were responsible for that in MCDs . - pubmed
Nine chlorinated metabolites produced from DDE were identified by gas chromatography - mass spectrometry - Fourier - transform infrared spectrometry ( GC - MS - FTIR ) from cultures grown on biphenyl . - pubmed
Role of quaternary structure in muscle creatine kinase stability : tryptophan 210 is important for dimer cohesion . - pubmed
A mutant of the dimeric rabbit muscle creatine kinase ( MM - CK ) in which tryptophan 210 was replaced has been studied to assess the role of this residue in dimer cohesion and the importance of the dimeric state for the native enzyme stability . - pubmed
All mutants exhibited wild - type preference for large hydrophobic residues , especially Phe , in the P1 ' substrate position . - pubmed
These amino acid positions are structurally equivalent to Arg8 , Leu10 , Val32 , Met46 , Ile54 , and Ile84 in HIV-1 PR , respectively , which mutate in drug - resistance . - pubmed
with the glucose antimetabolite , 2 - deoxy - D - glucose ( 2DG : 100 or 400 mg / kg ) , or the vehicle , saline , prior to onset of the expected LH surge . - pubmed
In other studies , i.c.v delivery of the opioid receptor antagonist , naltrexone ( NALT ) , partially reversed the inhibitory effects of 2DG on the gonadal steroid - induced LH surge . - pubmed
These studies show that magnitude of the LH surge is decreased by glucose substrate imbalance , and that regulatory effects of this metabolic challenge on the reproductive neuroendocrine axis is correlated with alterations in the transcriptional activation of preoptic GnRH neurones by gonadal steroid positive feedback . - pubmed
BM 144 : an original thromboxane A2 receptor antagonist derived from torasemide . - pubmed
With the aim to develop more potent TXA2 receptor antagonists , we investigated a series of torasemide derivatives . - pubmed
This pharmacomodulation led to the discovery of a sulfonyl - cyanoguanidine ( BM 144 ) which presents the same pharmacological profile as sulotroban , a TXA2 receptor antagonist used as reference . - pubmed
The retinoid concentrations returned to normal within 1 - 2 days post - irradiation , despite there being no overt increase in the uptake of [ 3H ] retinol or the biosynthesis of 3,4 - didehydroretinol . - pubmed
It has been known for many years that the absence of post - transcriptional modification at A1519 and the adjacent A1518 in strains lacking a functional KsgA methylase produces a kasugamycin resistance phenotype . - pubmed
Residues A794 and G926 are protected from chemical modification by kasugamycin and tRNA , and have been implicated in the initiation of protein synthesis . - pubmed
Post - natal modulation of heart and liver phosphoglyceride fatty acids in pups . - pubmed
Changes in fatty acid composition specific to tissue ( heart and liver ) and phosphoglyceride ( CPG and EPG ) occurred post - natally . - pubmed
With the exception in the heart EPG , the levels of arachidonic acid did not decrease concomitantly . - pubmed
Although the fatty acid composition of the diet did not change between pregnancy and lactation , docosahexaenoic and total n-3 increased in heart CPG and EPG and liver CPG of the suckling pups . - pubmed
The isoflavone genistein may either stimulate or inhibit cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl- channels . - pubmed
Single - channel analysis of channel block suggested that genistein ( 100 microM ) may inhibit CFTR by two mechanisms : first , it may slow the rate of channel opening and second , it may block open channels . - pubmed
Acidification of the intracellular solution relieved channel block , suggesting that the anionic form of genistein may inhibit CFTR . - pubmed
Genistein inhibition of CFTR Cl- currents was weakly voltage dependent and unaffected by changes in the extracellular Cl- concentration . - pubmed
Channel block was relieved by pyrophosphate ( 5 mM ) and ATP ( 5 mM ) , two agents that interact with the nucleotide - binding domains ( NBDs ) of CFTR to greatly stimulate channel activity . - pubmed
The data suggest that genistein may inhibit CFTR by two mechanisms . - pubmed
Second , it may bind within the CFTR pore to weakly block Cl- permeation . - pubmed
We proved a GC - MS - method with d ( 3 )- morphine as internal standard and silylation with MSTFA . - pubmed
Biologic effects included induction of low plasma concentrations of tumor necrosis factor - alpha and interleukin-6 observed immediately after MDXH210 infusion and 70 % saturation of circulating monocyte - associated Fc gamma RI with MDXH210 at a dose level of 4 to 8 mg / m2 . - pubmed
Quantitative determination of phospholipid compositions by ESI - MS : effects of acyl chain length , unsaturation , and lipid concentration on instrument response . - pubmed
DNA - XPA interactions : a ( 31 ) P NMR and molecular modeling study of dCCAATAACC association with the minimal DNA - binding domain ( M98 - F219 ) of the nucleotide excision repair protein XPA . - pubmed
Here , XPA - DNA interactions are further characterized by NMR spectroscopy from the vantage point of the DNA using a single - stranded DNA nonamer , dCCAATAACC ( d9 ) . - pubmed
A subset of ( 31 ) P resonances of d9 were observed to broaden and/or shift providing direct evidence that XPA - MBD binds d9 by a mechanism that perturbs the phosphodiester backbone of d9 . - pubmed
The interior five residues of d9 broadened and/or shifted before ( 31 ) P resonances of phosphate groups at the termini , suggesting that when d9 is bound to XPA - MBD the internal residues assume a correlation time that is characteristic of the molecular weight of the complex while the residues at the termini undergo a fraying motion away from the surface of the protein on a timescale such that the line widths are more characteristic of the molecular weight of ssDNA . - pubmed
A molecular model of the XPA - MBD complex with d9 was calculated based on the ( 15 ) N ( XPA - MBD ) and ( 31 ) P ( d9 ) chemical shift mapping studies and on the assumption that electrostatic interactions drive the complex formation . - pubmed
Even though the 4 - week and 8 - week exercise training caused 53 and 25 % increases , respectively , in total cytochrome P450 contents in the liver , exercise training did not cause any changes in the levels of P450 1A2 ( which primarily metabolizes azosemide ) , 2E1 and 3A23 in the liver , as assessed by both Western and Northern blot analyses . - pubmed
UNLABELLED : The standard celite or kaolin activated clotting time ( ACT ) correlates poorly with heparin levels during cardiopulmonary bypass ( CPB ) . - pubmed
We compared a modified kaolin ACT , in which plasma was supplemented , to a standard undiluted kaolin ACT for monitoring heparin levels during CPB . - pubmed
Heparin management was performed according to the Hepcon HMS results ( Medtronic , Minneapolis , MN ) . - pubmed
The data obtained were correlated to the plasma chromogenic anti - Xa activity as a reference assay for heparin levels . - pubmed
Methylation of lysine-79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast . - pubmed
Among these , the terminal transferase - mediated dUTP nick end - labeling ( TUNEL ) assay remains the most widely used technique . - pubmed
We developed a technique consisting of staining live cells and tissues with Hoechst 33342 and the vital dye propidium iodide ( PI ) , followed by fixation and the TUNEL reaction . - pubmed
Cusp - shaped conductivity spectra , consistent with weak impurity scattering of nodal quasiparticles , were obtained in the 0.6 - 21 GHz frequency range in highly ordered crystals of YBa2Cu3O6.50 and YBa2Cu3O6.99 . - pubmed
Eukaryotic initiation factor 5A ( eIF-5A ) contains an unusual amino acid , hypusine , which is formed post - translationally . - pubmed
Although eIF-5A and its hypusine modification are essential for eukaryotic cell viability , the precise physiological function of it has remained elusive . - pubmed
It is concluded that hypusine formation of eIF-5A exits in the regulation of cell cycle and the results suggest that eIF-5A is involved in the expression of proteins regulating transition of G(1)-S phase of cell cycle . - pubmed
[ USE OF CHLORDIAZEPOXIDE ( BALANCE ) AT UROLOGICAL CLINIC ] . - pubmed
Ganglioside GM3 blocks the activation of epidermal growth factor receptor induced by integrin at specific tyrosine sites . - pubmed
Previous studies have shown that ligand - dependent EGFR activation is inhibited by GM3 , the predominant ganglioside of epithelial cells , but the effect of GM3 on ligand - independent , integrin - EGFR cross - talk is unknown . - pubmed
Consistently , endogenous depletion of GM3 markedly increases the association of EGFR with tyrosine - phosphorylated integrin beta1 and promotes cell proliferation . - pubmed
Stimulation of EGFR and mitogen - activated protein kinase signaling by GM3 depletion involves the phosphorylation of EGFR at tyrosine residues 845 , 1068 , and 1148 but not 1086 or 1173 . - pubmed
The specific blockade of phosphorylation at Tyr-845 with Src family kinase inhibition and at Tyr-1148 with phosphatidylinositol 3 - kinase inhibition suggests that GM3 inhibits integrin - induced , ligand - independent EGFR phosphorylation ( cross - talk ) through suppression of Src family kinase and phosphatidylinositol 3 - kinase signaling . - pubmed
Taking 10(5 ) cells as a basis for comparisons , HAFs on PUF / SF exhibited initially higher glucose consumption rates , but persistently lower glutamine uptake rates than on PUF , whereas the rates of lactate and interleukin 6 release and of extracellular assembly of type I collagen fibers were alike on either substrate . - pubmed
This entry was attenuated by 2 - aminoethoxydiphenylborane , L - type Ca ( 2 + ) channel blockade , pinacidil , and Gd ( 3 + ) . - pubmed
The CCE - evoked contraction was sensitive to 2 - aminoethoxydiphenylborane , L - type Ca ( 2 + ) channel blockers , and Gd ( 3 + ) . - pubmed
E2 and E2 + P4 groups learned in fewer trials , and performed better in the radial maze and the working memory task than the other groups under the normal - calcium condition . - pubmed
In vitro treatment of embryonic pancreata with dexamethasone , a glucocorticoid agonist , induced a decrease of insulin - expressing cell numbers and a doubling of acinar cell area , indicating that glucocorticoids favored acinar differentiation ; in line with this , expression of Pdx-1 , Pax-6 , and Nkx6.1 was downregulated , whereas the mRNA levels of Ptf1 - p48 and Hes-1 were increased . - pubmed
We developed a diagnostic array of oligonucleotide probes targeting species - specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens . - pubmed
Both short- and long - term alcohol exposure also affect the serotonin receptors that convert the chemical signal produced by serotonin into functional changes in the signal - receiving cell . - pubmed
Pretreatment with protein kinase C ( PKC ) inhibitor blocked the TPA - induced increase in NAG-1 protein levels and NF - kappa B binding / transcriptional activity , whereas an inhibition of p38 , JNK , MEK activity had no effect on TPA - induced NAG-1 levels and NF - kappa B transcriptional activity . - pubmed
A single round of random mutagenesis followed by screening for hydrolytic activity for oleoyl 2 - naphthylamide as compared with that for oleoyl 2 - naphthyl ester identified five mutants with 1.7 - 2.0 - fold increased relative amidase activities . - pubmed
Active - site titration using a fluorescent phosphonic acid ester allowed the molecular activities for the amide and the ester to be determined for each mutant without purification of the lipase . - pubmed
A double mutant F207S / A213D gave the highest molecular activity of 1.1 min(-1 ) for the amide , corresponding to a 2 - fold increase compared with that of the wild - type lipase . - pubmed
A structural model of the lipase indicated that the mutations occurred at the sites near the surface and remote from the catalytic triad , but close to the calcium binding site . - pubmed
This study is a first step towards understanding why lipases do not hydrolyze amides despite the similarities to serine proteases in the active site structure and the reaction mechanism and towards the preparation of a general acyl transfer catalyst for the biotransformation of amides . - pubmed
Fluorescence quantum yield and lifetime of APL have been measured to be lower than those of the bare fluorophore ( AP ) due to the photoinduced intramolecular electron transfer ( PIET ) between the fluorophore and the receptor moieties of the molecule . - pubmed
Emerging evidence supports the recognition and management of secondary lipid goals , high - density lipoprotein cholesterol ( HDL - C ) level greater than 40 mg / dL , and triglyceride level less than 150 mg / dL. - pubmed
Preparation of the whole brain sample incorporated a highly efficient cysteinyl - peptide enrichment ( CPE ) technique to complement a global enzymatic digestion method . - pubmed
Both the global and the cysteinyl - enriched peptide samples were analyzed by SCX fractionation coupled with reversed phase LC - MS / MS analysis . - pubmed
RESULTS : By stepwise multivariate analyses , hemodynamic parameters such as left ventricular ejection fraction ( LVEF ) and left ventricular end - diastolic pressure ( LVEDP ) but not eGFR were independent predictors of a transcardiac increase ( coronary sinus - aortic root ) in BNP . - pubmed
Subtype specificity of scorpion beta - toxin Tz1 interaction with voltage - gated sodium channels is determined by the pore loop of domain 3 . - pubmed
A 22 - gauge needle was advanced to the sympathetic trunk at midlumbar level with CT fluoroscopic guidance , and 1 mL of iopamidol ( 200 mg of iodine per milliliter ) and 5 mL of 0.5 % bupivacaine were injected . - pubmed
Needle tip position and distribution of bupivacaine were measured on CT images . - pubmed
Genes of a glutathione S - transferase and a cytochrome P450 , which were induced by 2,4,6 - trinitrotoluene exposure in previous studies , were upregulated by 10- and 8 - fold , respectively . - pubmed
pneumophila secretes a siderophore ( legiobactin ) that is detectable by the CAS assay . - pubmed
The lbtA mutant is also defective for growth in deferrated media containing citrate , indicating that legiobactin is required in conditions of severe iron limitation . - pubmed
pneumophila does express lbtA in macrophages , suggesting that legiobactin has a dispensable role in infection . - pubmed
Anion - exchange HPLC has been used to purify legiobactin , and thus far , structural analysis suggests that the molecule is similar but not identical to rhizobactin , rhizoferrin , and alcaligin . - pubmed
pneumophila cytochrome c maturation locus in low - iron growth , intracellular infection , and virulence . - pubmed
STUDY DESIGN : Retrospective observational analysis of claims - based data of health plan members with CAD receiving 1 or more prescriptions per year of any of the following classes of medications used for secondary prevention of CAD : lipid - lowering agents , angiotensin - converting enzyme ( ACE ) inhibitors , or angiotensin receptor blockers ( ARBs ) and beta - blockers . - pubmed
Thus , CD4 T cells from IPF patients have characteristics typical of cell - mediated pathologic responses , including augmented effector functions , provision of facultative help for autoantibody production , oligoclonal expansions , and proliferations driven by an Ag present in diseased tissues . - pubmed
AIM : To evaluate promotive effect of hyperthermia on the carcinostatic activity of synthesized omega - hydroxy fatty acids ( omega HFAs ) and their ethylesters agaist Ehrlich ascites tumor ( EAT ) cells . - pubmed
METHODS : EAT cells were cultured with either omegaHFAs or their ethylester derivatives in a water bath at either 37 degrees C or 42 degrees C for 30 min , followed by incubation in a CO2 incubator for 20 or 72 h. Mitochond - rial dehydrogenase - based WST-1 assay and trypan blue dye exclusion assay were then conducted after incubation . - pubmed
An autoimmune process was considered to be responsible for the repeated episodes of encephalitis because the symptoms were highly responsive to steroids and anti - glutamate receptor antibodies were detected in the CSF . - pubmed
Thiopurine - methyltransferase and inosine triphosphate pyrophosphatase polymorphism in a liver transplant recipient developing nodular regenerative hyperplasia on low - dose azathioprine . - pubmed
The enzymes thiopurine - methyltransferase ( TPMT ) and inosine triphosphate pyrophosphatase ( ITPA ) are involved in thiopurine metabolism . - pubmed
Geologic estimates suggest that atmospheric oxygen in the late Paleozoic Era was 35 % . - pubmed
The concentration of GABA is modulated by the pyridoxal-5 '- phosphate ( PLP)-dependent enzymes , GAD and GABA - T. In this study , we generated pyridoxyl - gamma - aminobutyrate ( PL - GABA ) , a novel GABA analogue composed of pyridoxyl and GABA , and have also characterized its anticonvulsant and pharmacological functions in vitro . - pubmed
The results of biodistribution studies revealed that PL - GABA is capable of crossing the blood - brain barrier . - pubmed
PL - GABA evidenced anticonvulsant activity in a wide range of epilepsy models , some of which were electrically - based ( MES seizures ) and some chemically - based ( bicuculline , pentylenetetrazol ( PTZ ) , picrotoxine , 3 - mercaptopropionic acid ) . - pubmed
In addition , PL - GABA displayed no signs of tolerance after subchronic ( 10 day ) treatment . - pubmed
Therefore , our results indicate that PL - GABA exerts a broad - spectrum anticonvulsant effect , and identify the potential for reduced PL - GABA tolerance as an additional positive profile for novel antiepileptic drugs . - pubmed
Hemoglobin - based oxygen carriers may play a future role as intravascular volume expanders in lieu of transfusion of red blood cell concentrates . - pubmed
The recovery of Amberlite XAD-16 purified FA was up to 57.97 % with HPLC purity 50.89 % . - pubmed
The role of the heterocycle in bis ( hydroxyphenyl ) triazoles for inhibition of 17beta - Hydroxysteroid Dehydrogenase ( 17beta - HSD ) type 1 and type 2 . - pubmed
17beta - Hydroxysteroid dehydrogenase type 1 ( 17beta - HSD1 ) is responsible for the catalytic reduction of the weak estrogen estrone ( E1 ) into the highly potent 17beta - estradiol ( E2 ) . - pubmed
As 17beta - HSD1 is often overexpressed in mammary tumors and endometriosis , the selective inhibition of this enzyme is discussed as a promising approach for the treatment of estrogen - dependent diseases . - pubmed
Recently , we reported on bis ( hydroxyphenyl ) azoles as a new class of potent inhibitors of 17beta - HSD1 . - pubmed
Substituents were introduced on the triazole core in order to establish additional interactions with the enzyme active site . - pubmed
3 -[ 4 -( 4 - Hydroxyphenyl )- 1H-1,2,3 - triazol-1 - yl ] phenol ( 3 ) was the most active compound discovered in this study with an IC(50 ) value of 840nM and a reasonable selectivity towards 17beta - HSD2 . - pubmed
CONCLUSIONS : These data show that dual gene therapy with ER / GR might be a useful tool for understanding the role of steroid hormones in the storage of traumatic memories . - pubmed
Huntington ' s disease is a neurodegenerative disorder caused by a polyglutamine expansion within the huntingtin protein . - pubmed
Blood glucose and insulin levels and various appetite hormone concentrations were measured during the study . - pubmed
Bone metabolism was evaluated by serum calcium , phosphorus , alkaline phosphatase , parathyroid hormone , 25 ( OH ) vitamin D , osteocalcin , serum C - telopeptide cross - linked collagen type I levels . - pubmed
Validation of the mechanism of cholesterol binding by StAR using short molecular dynamics simulations . - pubmed
We previously proposed an original two - state cholesterol binding mechanism by StAR , in which the C - terminal alpha - helix of StAR gates the access of cholesterol to its binding site cavity . - pubmed
In our molecular model of the cholesterol - StAR complex , the hydrophobic moiety of cholesterol interacts with hydrophobic amino acid side - chains located in the C - terminal alpha - helix and at the bottom of the cavity . - pubmed
Molecular dynamics simulations showed that point mutations of Phe(267 ) , Leu(271 ) or Leu(275 ) at the alpha - helix 4 increased the gyration radius ( more flexibility ) of the protein 's structure , whereas the salt bridge double mutant E169M / R188 M showed a decrease in flexibility ( more compactness ) . - pubmed
Endothelial dysfunction is intricately related to insulin resistance through the parallel stimulatory effects of insulin on glucose disposal in metabolic tissues and NO production in the endothelium . - pubmed
In all cases , the prolines pucker substantially and the pucker is associated with the backbone rotation toward the E2 / ubiquitin . - pubmed
In contrast , NALP3 functionality in intermediate stage melanoma cells requires activation of the IL-1 receptor to secrete active IL-1beta ; cells from an early stage of melanoma require stimulation of the IL-1 receptor plus the co - stimulant muramyl dipeptide . - pubmed
Discovery of 7 -( 4 -( 3 - ethynylphenylamino )- 7 - methoxyquinazolin-6 - yloxy )- N - hydroxyheptanamide ( CUDc-101 ) as a potent multi - acting HDAC , EGFR , and HER2 inhibitor for the treatment of cancer . - pubmed
By incorporating histone deacetylase ( HDAC ) inhibitory functionality into the pharmacophore of the epidermal growth factor receptor ( EGFR ) and human epidermal growth factor receptor 2 ( HER2 ) inhibitors , we synthesized a novel series of compounds with potent , multiacting HDAC , EGFR , and HER2 inhibition and identified 7 -( 4 -( 3 - ethynylphenylamino )- 7 - methoxyquinazolin-6 - yloxy )- N - hydroxyheptanamide 8 ( CUDC-101 ) as a drug candidate , which is now in clinical development . - pubmed
Uncontrolled studies suggest that prostacyclin analogues and phosphodiesterase type-5 inhibitors may have benefits in advanced pulmonary vascular disease . - pubmed
Using a BGE of 100 mM acetic acid ( pH 3.1 ) and a positively charged capillary coating , a highly reproducible and efficient separation of four model proteins ( insulin , carbonic anhydrase II , ribonuclease A and lysozyme ) was obtained . - pubmed
Sheath - liquid CE - MS using the same porous tip capillary and an isopropanol - water - acetic acid sheath liquid showed slightly lower to similar analyte responses . - pubmed
The analyte response in sheathless CE - MS could be enhanced using a nanoESI source and adding 5 % isopropanol to the BGE , leading to improved detection limits by 50 - fold to 140 - fold as compared to sheath liquid interfacing using the same capillary - equivalent to sub - nM detection limits for three out of four proteins . - pubmed
Sauchinone protects pancreatic beta cells against cytokine - mediated toxicity . - pubmed
In this study , we investigated the effect of sauchinone on IL-1beta ( 5 ng / ml ) and IFN - gamma ( 100 U / ml)-induced beta - cell damage . - pubmed
Sauchinone prevented nitric oxide ( NO ) production , and this effect was correlated with reduced levels of protein expression of the inducible form of NO synthase ( iNOS ) . - pubmed
The molecular mechanism by which sauchinone inhibits iNOS gene expression appeared to involve the inhibition of NF - kappaB activation . - pubmed
Moreover , pancreatic beta - cells treated with cytokines upregulated the phosphorylation of STAT-1 , STAT-3 and STAT-5 , however , pre - treatment with sauchinone attenuated these effects . - pubmed
Sauchinone prevented cytokine - induced NO production , iNOS expression , JAK / STAT activation , and NF - kappaB activation and inhibition of glucose - stimulated insulin secretion ( GSIS ) . - pubmed
Even though , it has been shown that TiO ( 2 ) NPs exposure can induce some alterations in mitochondria including cytochrome c release to cytosol , change in mitochondrial permeability and decrease of mitochondrial membrane potential ( DeltaPsi(m ) ) , there is no information about the changes in mitochondrial function induced by TiO ( 2 ) NPs . - pubmed
[ Effect of vitamin C on the condition of NO - synthase system in experimental stomach ulcer ] . - pubmed
Vit C displayed a pronounced antioxidant action , reduced the degree of destructive affections , diminished the activity of iNOS and lipoperoxidation processes , decreased the NO content and SOD activity . - pubmed
Combined action of Vit C with L - arginine reduced the degree of GM lesions , activity of eNOS and the content of NO in GM whereas the concentration of L - arginine in blood was increased . - pubmed
Under conditions of Vit C action and iNOS and COX-2 blockage , the activity of NO - synthases and lipoperoxidation processes were slightly decreased , indicating on dominant action of Vit C. - pubmed
Here , we describe an engineered split - adaptor system , in which adaptor assembly and delivery of substrates to the ClpXP protease depends on a small molecule ( rapamycin ) . - pubmed
Seasonal patterns of prolactin and corticosterone secretion in an Antarctic seabird that moults during reproduction . - pubmed
In avian species that have evolved life - history strategies wherein molt and breeding overlap , there are potential conflicts between the regulatory roles of baseline prolactin and corticosterone in parental care ( positive ) and moult ( negative ) . - pubmed
When comparing prolactin , corticosterone , and moult in failed vs. active breeders , we found that failed breeding enabled a more rapid down - regulation of prolactin , thus facilitating a more rapid moult . - pubmed
Enhancing microRNA transfection to inhibit survivin gene expression and induce apoptosis : could it be mediated by a novel combination of sonoporation and polyethylenimine ? - pubmed
The AT - rich interactive domain 1A gene ( ARID1A ) , which encodes one of the subunits in the Switch / Sucrose Nonfermentable chromatin remodeling complex , carries mutations and is responsible for loss of protein expression in gastric carcinoma , particularly with Epstein - Barr virus ( EBV ) infection and a microsatellite instability - high phenotype . - pubmed
OBJECTIVE : The purpose of our study was to prospectively and directly compare capability of O2 - enhanced MRI , MDCT , and perfusion SPECT / CT to clinical outcome measurements in candidates for lung volume reduction surgery ( LVRS ) . - pubmed
Quantitatively assessed upper / lower lung ratios on O2 - enhanced MRI , MDCT , and SPECT / CT were calculated from regional relative enhancement ratios , functional lung volumes , and radioisotope uptakes between upper and lower lungs . - pubmed
Qualitatively assessed upper / lower lung ratios on O2 - enhanced MRI , MDCT , and SPECT / CT were estimated using visual scoring systems . - pubmed
CONCLUSION : O2 - enhanced MRI shows potential for more accurate evaluation of postoperative clinical outcome for LVRS candidates than SPECT / CT and can be considered at least as reliable as MDCT . - pubmed
IMPACT : Adequate folate status may protect against colorectal carcinogenesis through mechanisms involving adequate DNA methylation in the genome . - pubmed
Blood samples were also collected for determining FBS , HbA1c , cholesterol and triglyceride . - pubmed
Blood lead , delta - aminolevulinic acid dehydratase ( delta - ALAD ) and zinc protoporphyrin ( ZPP ) concentrations are widely used as biomarkers for lead toxicity The present study was designed to determine the impact of chronic lead exposure on selected biological markers . - pubmed
Reflectance difference spectroscopy ( RDS ) is applied to follow in situ the preparation of clean and carburized W ( 1 1 0 ) surfaces and to study the temperature - induced transition between the R(15 x 3 ) and R(15 x 12 ) carbon / tungsten surface phases . - pubmed
alpha - Lipoic acid ameliorates mitochondrial impairment and reverses apoptosis in FABP3 - overexpressing embryonic cancer cells . - pubmed
Fatty acid - binding protein 3 ( FABP3 ) is a low molecular weight protein with distinct tissue distribution , which may play an important role in fatty acid transport , cell growth , cellular signaling , and gene transcription . - pubmed
Furthermore , the MMP , intracellular ATP synthesis and the amount of mitochondrial DNA were also increased . - pubmed
Collectively , our results indicate that abnormalities in FABP3 expression contribute to mitochondrial dysfunction and apoptosis , and that alpha - LA represents a suitable candidate for development as a treatment for apoptosis - related congenital cardiac malformations . - pubmed
Calprotectin ( S100A8 / A9 ) , S100A12 , and EDTA - resistant S100A12 complexes ( ERAC ) in primary Sjogren ' s syndrome . - pubmed
Injury - mediated increased K (+ ) efflux through Kv2.1 channels promotes neuronal apoptosis , contributing to widespread neuronal loss in neurodegenerative disorders such as Alzheimer ' s disease and stroke . - pubmed
Diabetic patients have characteristic pattern of dyslipidemia with decreased level of high density lipoprotein cholesterol ( HDL - C ) and elevated triglycerides ( TG ) level . - pubmed
However , in diabetes mellitus , low density lipoprotein cholesterol ( LDL - C ) which is used as one of the markers for the risk of CVD , is underestimated so in such cases the levels of non - High density lipoprotein cholesterol ( non - HDL - C ) can be a stronger predictor of CVD as it strongly correlates with atherogenic lipoproteins . - pubmed
Diabetics have significantly elevated ratio of total cholesterol to high density lipoprotein cholesterol ( TC / HDL - C ) and the significant increased levels of non - high density lipoprotein cholesterol ( non - HDL - C ) compared to controls which can be used as markers of dyslipidemia and can also be used to predict the risk of cardiovascular disease in type 2 Diabetes Mellitus . - pubmed
18 - Fluorine fluorodeoxyglucose positron emission tomography in the pretreatment evaluation of thymic epithelial neoplasms : a metabolic biopsy confirmed by Ki-67 expression . - pubmed
OBJECTIVES : To investigate the usefulness of 18 - fluorine fluorodeoxyglucose ( ( 18 ) F - FDG ) positron emission tomography / computed tomography ( PET - CT ) in the pretreatment evaluation of thymic epithelial neoplasms ( TENs ) . - pubmed
CONCLUSIONS : FDG PET - CT can provide a useful tool in the preoperative work - up of TEN , reflecting its proliferation capacity , as described also by the Ki-67 expression . - pubmed
The major components of C - CAF are oxidative tea polyphenols ( OTP ) and caffeine . - pubmed
In this study , we treated MUCs with trichostatin A ( TSA ) to determine whether histone deacetylase inhibitor is able to stimulate the expression of epithelial genes in MUCs , an essential step for guiding mesenchymal - like MUCs to become sensory epithelial cells . - pubmed
After 72 h of TSA treatment , MUCs acquired epithelial - like features , which were indicated by increased expression of epithelial markers such as Cdh1 , Krt18 , and Dsp . - pubmed
Additionally , TSA decreased the expression of mesenchymal markers , including Zeb1 , Zeb2 , Snai1 , and Snai2 , and prosensory genes Lfng , Six1 , and Dlx5 . - pubmed
Moreover , the expression of the hair cell genes Atoh1 and Myo6 was increased in TSA - treated MUCs . - pubmed
We also observed significantly decreased expression of Hdac2 and Hdac3 in TSA - treated MUCs . - pubmed
However , no remarkable change was detected in protein expression using immunofluorescence , indicating that TSA - induced HDAC inhibition may contribute to the initial stage of the mesenchymal - to - epithelial phenotypic change . - pubmed
Nucleotide comparison revealed no significant similarity between bsh1 and bsh2 genes , whereas the deduced amino acid sequences showed 26 % sequence similarity between both BSH1 and BSH2 . - pubmed
Furthermore , examination of metabolic intermediates by gas chromatography - mass spectrometry revealed that the naphthalene metabolic pathway in transgenic plants mainly involves the dioxygenase pathway . - pubmed
Taken together , our findings suggest that grafting the naphthalene dioxygenase complex into plants is a possible strategy to breed PAH - tolerant plants to efficiently degrade PAHs in the environment . - pubmed
Using collagen ultrafiltration and single amino acid ( hydroxyproline ) methods , these specimens consistently date to beyond or near the ~ 50,000 y B.P. - pubmed
OBJECTIVE : To evaluate the efficacy and safety of the combination of steroids , plasmapheresis and intravenous immunoglobulins ( IVIG ) on maternal anti Ro / SS - A antibody levels in cases of fetal cardiac involvement . - pubmed
MATERIAL AND METHODS : A series of three cases of positive anti - Ro / SS - A mothers with fetuses showing mild cardiac involvement were treated with a triple therapy composed of steroids , plasmapheresis and IVIG . - pubmed
Caffeine and caffeic acid suppressed the growth of ER(+ ) ( P & lt;= 0.01 ) and ER(- ) ( P & lt;= 0.03 ) cells . - pubmed
Caffeine significantly reduced ER and cyclin D1 abundance in ER(+ ) cells . - pubmed
Caffeine also reduced the insulin - like growth factor - I receptor ( IGFIR ) and pAkt levels in both ER(+ ) and ER(- ) cells . - pubmed
CONCLUSIONS : The clinical and experimental findings demonstrate various anticancer properties of caffeine and caffeic acid against both ER(+ ) and ER (- ) breast cancer that may sensitize tumor cells to tamoxifen and reduce breast cancer growth . - pubmed
RESULTS : For the rocuronium - induced neuromuscular block , the mean recovery time of the T4 / T1 ratio to 0.9 decreased from 66.9 min in the sugammadex 0.5 mg kg-1 group to 1.3 min in the sugammadex 8.0 mg kg-1 group . - pubmed
Implementation of diesel particulate filters for goods vehicles in the 2021 - ALT - IV - O scenario would help in the reduction of ~87 % of PM2.5 concentration , compared to the 2021 - BAU scenario ; translating into a gain of 1267 and 505 DALY per million people from exposure to PM2.5 and NO2 concentrations , respectively . - pubmed
After calculating these spectral indices based on the resampling TM and Hyperion data , the regression equation between spectral indices and chlorophyll content was established . - pubmed
For TM , the result indicates that VIUPD has the best correlation with chlorophyll ( R2 = 0.819 7 ) followed by NDVI ( R2 = 0.791 8) , while MCARI / OSAVI and TVI also show a good correlation with R2 higher than 0.5 . - pubmed
It was demonstrated that VIUPD has the best accuracy and stability to estimate chlorophyll of winter wheat whether using simulated TM data or Hyperion data , which reaffirms that VIUPD is comparatively sensor independent . - pubmed
Simple correlation analyses showed that serum pentosidine levels were significantly and negatively correlated with muscle mass of legs ( r=-0.21 , p=0.017 ) and RSMI ( r=-0.18 , p=0.022 ) , and that IGF - I was significantly and positively correlated with muscle mass of arms and legs ( r=0.23 , p=0.008 and r=0.30 , p=0.001 , respectively ) as well as RSMI ( r=0.20 , p=0.022 ) . - pubmed
Moreover , after adjusting for age , duration of diabetes , serum creatinine , HbA1c , and IGF - I , pentosidine was significantly and negatively associated with RSMI ( beta=-0.27 , p=0.018 ) and marginally with muscle mass of legs ( beta=-0.18 , p=0.071 ) . - pubmed
The associations between IGF - I and indices of muscle mass such as arms , legs and RSMI were still significant after additional adjustment for pentosidine ( p=0.016 , 0.019 and 0.021 , respectively ) . - pubmed
These findings indicate that increased serum pentosidine and decreased IGF - I are independent risk factors for loss of muscle mass in postmenopausal women with type 2 diabetes . - pubmed
Randomized Controlled Trial of Zoledronic Acid plus Chemotherapy versus Chemotherapy Alone as Neoadjuvant Treatment of HER2 - Negative Primary Breast Cancer ( JONIE Study ) . - pubmed
We performed the present randomized Phase 2 trial to investigate the antitumor effect of ZOL with chemotherapy ( CT ) . - pubmed
Sonochemical enzyme - catalyzed regioselective acylation of flavonoid glycosides . - pubmed
The data suggested that monoclonal sclerostin - neutralizing antibody represents a promising therapeutic approach for severe osteoporosis induced by estrogen deficiency with concurrent mechanical unloading . - pubmed
However , unlike other HSPs such as HSP90 and HSP70 , small molecule approaches for neutralization of HSP27 are not well established because of the absence of an ATP binding domain . - pubmed
Previously , we found that a small molecule , zerumbone ( ZER ) , induced altered dimerization of HSP27 by cross linking the cysteine residues required to build a large oligomer , led to sensitization in combination with radiation . - pubmed
Dysregulation of matriptase expression , an epithelial - specific member of the type II transmembrane serine protease family , has been demonstrated in many different cancer types . - pubmed
Mass yield of mevalonate was increased by 41 % , and production was maintained for more than 45h after activation of the pyrF - based growth switch . - pubmed
Lipidomic analyses of the two mutants revealed significant and specific declines in phosphatidylinositol and phosphatidylglycerol levels upon repression of TgCDS1 and after deletion of TgCDS2 , respectively . - pubmed
Here we show that perturbations in equilibrium picosecond - nanosecond motions impact zinc ( Zn)-induced allosteric inhibition of DNA binding by the Zn efflux repressor CzrA ( chromosomal zinc - regulated repressor ) . - pubmed
Allosterically impaired CzrA mutants are characterized by distinct nonnative fast internal dynamics " fingerprints " upon Zn binding , and DNA binding is weakly regulated . - pubmed
These findings identify a unique amplification process of TNF synthesis and secretion via the interaction of NK-1 and ST2 receptors inhibitable by methoxyluteolin . - pubmed
Three sequence - related amplified polymorphism ( SRAP ) markers were employed to fingerprint genomic DNA , and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high - performance liquid chromatography . - pubmed
Mn - induced mitochondria - related apoptotic characteristics , such as caspase-3 and - 9 activation , cytochrome c release , Bax increase , and Bcl-2 decrease , were significantly suppressed by curcumin . - pubmed
In addition , curcumin significantly increased intracellular glutathione ( GSH ) and moderately potentiated superoxide dismutase ( SOD ) , both which were diminished by Mn treatment . - pubmed
Furthermore , curcumin was able to induce heme oxygenase ( HO )- 1 expression . - pubmed
Curcumin - mediated inhibition of ROS , down - regulation of caspases , restoration of MMP , and recovery of cell viability were partially reversed by HO-1 inhibitor ( SnPP ) . - pubmed
These results suggest the first evidence that curcumin can prevent Mn - induced microglial cell death through the induction of HO-1 and regulation of oxidative stress , mitochondrial dysfunction , and apoptotic events . - pubmed
Stimulatory Action of Telmisartan , an Antagonist of Angiotensin II Receptor , on Voltage - Gated Na + Current : Experimental and Theoretical Studies . - pubmed
Telmisartan ( Tel ) is recognized as a non - peptide blocker of AT1R. - pubmed
Mechanistically , Snhg8 sustained SCs proliferation by promoting the transcription of ribosomal proteins , while Gm26917 acted as a competing endogenous RNA for microRNA-29b , which accelerated apoptosis of SCs . - pubmed
Forward contamination is prevented with sealed tubes , and the use of thermolabile uracil DNA glycosylase ( UDG ) in the presence of dUTP in the amplification mixture . - pubmed
A long - lasting oral preformulation of the angiotensin II AT1 receptor antagonist losartan . - pubmed
Losartan ( Los ) , a non - peptidic orally active agent , reduces arterial pressure through specific and selective blockade of angiotensin II receptor AT1 . - pubmed
Here , we show that native transition metals ( Fe0 , Ni0 and Co0 ) selectively reduce CO2 to acetate and pyruvate - the intermediates and end - products of the AcCoA pathway - in near millimolar concentrations in water over hours to days using 1 - 40 bar CO2 and at temperatures from 30 to 100 C. Geochemical CO2 fixation from native metals could have supplied critical C2 and C3 metabolites before the emergence of enzymes . - pubmed
The present study for the first time gives an insight into regulation of estrogen - related receptors by histone methylation specifically through methyltransferase EZH2 in breast cancer . - pubmed
Immunohistochemistry in primary breast tumors of different grades showed a correlated expression of estrogen - related receptors and EZH2 . - pubmed
Ectopic expression of EZH2 in low EZH2 - expressing normal breast epithelial cells abrogated their expression and at the same time , its silencing enhanced the expression of estrogen - related receptors in cancerous cells . - pubmed
IE measurements with liquid chromatography electrospray ionization mass spectrometry ( LC / ESI / MS ) were carried out , and IE scales were constructed with a calibration curve for compounds with and without derivatization reagent diethyl ethoxymethylenemalonate . - pubmed
Abrogation of the Circadian Nuclear Receptor REV - ERBalpha Exacerbates 6 - Hydroxydopamine - Induced Dopaminergic Neurodegeneration . - pubmed
REV - ERBalpha deficiency significantly exacerbated 6 - OHDA - induced motor deficits as well as DAergic neuronal loss in the vertebral midbrain including the SN and the ventral tegmental area . - pubmed
In conclusion , the present study demonstrates for the first time that genetic abrogation of REV - ERBalpha can increase vulnerability of DAergic neurons to neurotoxic insults , such as 6 - OHDA , thereby implying that its normal function may be beneficial for maintaining DAergic neuron populations during PD progression . - pubmed
Decreased reduction rate of the luciferase flavin substrate was shown to be a possible reason for bioluminescence quenching . - pubmed
Furthermore , ATG7 knockdown led to an increased survival fraction under oxygen and glutamine starvation , indicating that ionizing radiation indeed induces autophagy which , however , leads to cell death finally . - pubmed
Van der Waals heterostructures composed of two - dimensional ( 2D ) transition metal dichalcogenides ( TMD ) materials have stimulated tremendous research interest in various device applications , especially in energy - efficient future - generation electronics . - pubmed
Long - term improvement in renal , bone , lipid parameters , and CD4 / CD8 ratio in HIV - infected patients switching to a dual therapy with lamivudine plus boosted darunavir . - pubmed
Thiol - norbornene photoclick hydrogels were formed with CRGDS and crosslinkers of PEG dithiol ( nondegradable ) , CVPLS - LYSGC ( P1 ) or CRGRIGF - LRTDC ( P2 ; dash indicates cleavage site ) at two crosslink densities . - pubmed
Overall , the low crosslinked P1 hydrogels exhibited an accelerated early and late osteogenesis with the highest ALP activity ( Day 7 ) , greatest calcium content ( Day 14 ) , and greatest collagen content ( Day 28 ) , concomitant with increased compressive modulus over time . - pubmed
The infant grew rapidly with rising PRL , GH , and IGF-1 concentrations and an enlarging suprasellar pituitary mass , despite treatment with bromocriptine . - pubmed
Here we predict two monolayered aluminum triphosphides ( AlP3 ) with C2 / m and P3m1 space groups with a tunable bandgap under strain as the new members of the 2D XP3 family by using the first principles calculations . - pubmed
The high mobility of 1.01 x 105 and 1.62 x 104 cm2 V-1 s-1 are observed for the electron of P3m1 and the hole of C2 / m. The optical absorptions of the AlP3 monolayers , in particular , the one with C2 / m , are obviously strong in the visible light range . - pubmed
The three - year concentration of PM2.5 , PM10 and NO2 was 72.8 + - 2.3 mug / m3 , 131.5 + - 5.7 mug / m3and 39.1 + - 3.1 mug / m3 , respectively . - pubmed
Complete ( 100 % ) inhibition of biofilm formation was observed with plain CuO NP ( 0.5 M hexamine , uncapped ) at 1,000 microg ml-1 . - pubmed
In this study , we evaluated the effects of PPB effect M1 polarization and inflammation and its ability to restore the effects of leptin , such as a decrease in appetite and body weight . - pubmed
Losatuxizumab vedotin ( formerly ABBV-221 ) is a second - generation antibody - drug conjugate targeting epidermal growth factor receptor ( EGFR ) . - pubmed
The NLRP3 ( nucleotide - binding domain , leucine - rich - repeat - containing family , pyrin domain - containing 3 ) inflammasome senses pathogen - associated molecular patterns ( PAMPs ) and danger - associated molecular patterns ( DAMPs ) , and activates caspase-1 , which provokes release of proinflammatory cytokines such as interleukin-1beta ( IL-1beta ) and IL-18 as well as pyroptosis to engage in innate immune defense . - pubmed
ER - mitochondria contact sites provide a location for NLRP3 activation , its association with ligands released from or residing in mitochondria , and rapid Ca2 + mobilization from ER stores to mitochondria . - pubmed
Lipid perturbation and cholesterol trafficking to the ER activate the NLRP3 inflammasome . - pubmed
Serum NO and H2O2 concentrations increased significantly and were related to muscle damage ( CK activity > 900 IU / mL ) in IHE and Arg / IHE compared to placebo . - pubmed
The changes in NO and H2O2 elevated the levels of circulating growth factors such as HGF , IHG-1 , PDGFBB , BDNF , VEGF and EPO . - pubmed
Pembigatinib has become the first approved targeted therapy for BTC with FGFR2 fusion or other rearrangements . - pubmed
Recent immunotherapy has opened new therapy avenues in BTC with pembrolizumab approved for either microsatellite instability high ( MSI - H ) or DNA mismatch repair deficient ( dMMR ) advanced solid tumors , including BTC . - pubmed
Excellent therapeutic success of the anti - interleukin 4 receptor - alpha antibody dupilumab in clinical trials and a real - world clinical context indicates the crucial roles of interleukin ( IL )- 4 and IL-13 in the pathogenesis of AD . - pubmed
Along with the clinical improvement in skin scores and pruritus , dupilumab significantly and progressively reduces and normalizes the upregulated expression of T helper type 2 signatures such as Chemokine ( C - C motif ) ligand ( CCL ) 17 , CCL18 , CCL22 , and CCL26 in the lesional skin of AD . - pubmed
Herein , we report a nano - MOF conjugated to maltotriose as a new DDS . - pubmed
This novel MOF is an effective PDT agent and shows little dark toxicity , MA - PCN-224 - 0.1Mn/0.9Zn uptakes selectively into cancer cells . - pubmed
Aberrant ALDH5A1 promoter regulation in functional terms can contribute to impaired gamma - aminobutyric acid homeostasis and thereby network excitability and seizure propensity . - pubmed
The inhibition effect of N , N '- phosphonomethylglycine ( PMG ) and vinyl phosphonic acid ( VPA ) on the 3 % NaCl acidic solution corrosion of carbon steel iron was studied at different immersion times by potentiodynamic polarization , electrochemical impedance spectroscopy , attenuated total reflectance Fourier transform infrared ( ATR - FTIR ) spectroscopy , and computational methods . - pubmed
Plasma biochemical analytes included glucose ( Glu ) , total calcium , phosphorus , sodium , potassium , chloride , blood urea nitrogen ( BUN ) , uric acid , cholesterol , triglycerides , creatine kinase , gamma - glutamyl transferase , alkaline phosphatase ( ALP ) , lactate dehydrogenase , aspartate aminotransferase , amylase , and bile acids . - pubmed
Moreover , IMO decreased the concentrations of serum D - lactate ( D - LA ) and lipopolysaccharides ( LPS ) at farrowing and day 18 of lactation ( L18 ) ( P < 0.05 ) . - pubmed
At 48 h before CIDR withdrawal , does were treated with 80 mg follicle - stimulating hormone ( FSH ) administered at 12 h intervals . - pubmed
Carbon black filled only the pial arteries and the rest of the brain was totally unperfused . - pubmed
Effects of dietary magnesium supplementation on diurnal variations of blood pressure and plasma Na + , K (+)- ATPase activity in essential hypertension . - pubmed
Supplementation of MgO elicited a significant fall in averaged mean blood pressure calculated with a 24 - h ambulatory blood pressure monitoring system ( MBP ) in EH from a baseline value of 104.3 + /- 12.2 to 99.5 + /- 11.6 mmHg ( p & lt ; 0.05 ) , while controls remained unaltered from a baseline value of 85.1 + /- 11.5 to 84.5 + /- 13.3 mmHg . - pubmed
After 2 weeks of magnesium supplementation , the plasma level of Na + , K (+)- ATPase inhibitory activity ( PATPI ) , defined as equivalency to ouabain , was reduced significantly from 0.75 + /- 0.54 to 0.40 + /- 0.30 mumol ouabain / ml ( p & lt ; 0.05 ) in the responder group , while it remained unaltered in controls and the nonresponder group . - pubmed
A significant negative correlation existed between the pretreatment PRA and changes in MBP after magnesium supplementation in EH ( r = -0.65 , p & lt ; 0.01 ) , and there was a significant positive correlation between changes in PATPI and changes in MBP as a whole ( r = 0.41 , p & lt ; 0.05 ) . - pubmed
It appears that magnesium suppresses circulating Na + , K (+)- ATPase inhibitory activity to attenuate vascular tone , and thereby reduces blood pressure in EH . - pubmed
The enzyme displayed the maximum activity towards proline p - nitroanilide at pH 7.7 - 7.9 and readily split glycine , alanine and proline from di - , tri - and tetrapeptides but did not practically hydrolyze oligopeptides of a greater chain length . - pubmed
The enzyme was not inhibited by complexons ( EDTA , 8 - oxiquinoline and 1.10 - phenanthroline ) . - pubmed
The peptidase was not activated by divalent metal ions and was inhibited by Zn2 + ; Cd2 + , Hg2 + and Cu2 + . - pubmed
In the presence of sodium dodecyl sulfate the molecular mass of the enzyme is 43 kDa , which is suggestive of the presence of a quaternary structure . - pubmed
One peculiarity of the enzyme is its activation by alkaline metal halogenides and sodium nitrate which reaches a maximum at the 0.05 - 0.1 M concentration of the salts . - pubmed
Under conditions of exposure where both chemicals elicited an IgG anti - hapten antibody response , only phthalic anhydride caused an increase in the serum concentration of IgE. - pubmed
Furthermore , like TMA , phthalic anhydride preferentially induced IgG2b rather than IgG2a antibody . - pubmed
In contrast , oxazolone , like DNCB , induced a markedly stronger IgG2a than IgG2b antibody response . - pubmed
The HOX4A protein predicted from the nucleotide sequence of the HOX4A gene is comprised of 416 amino acid residues . - pubmed
A dose finding study of a super long - acting luteinizing hormone - releasing hormone analog ( leuprolide acetate depot , TAP-144 - SR ) in the treatment of central precocious puberty . - pubmed
The lack of PLA2 in salt extracts from newborn cartilage observed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis analysis was confirmed by the very low levels of PLA2 activity detectable in these preparations . - pubmed
ER mRNA containing cells were found in the periventricular , suprachiasmatic and medical preoptic cell groups , in a manner which agrees with steroid hormone autoradiography . - pubmed
We conclude that the insensitivity to the male to progesterone , as regards the hormonal control of ovulation , can not be due to a total failure of PR gene expression in a specific subset of POA neurons . - pubmed
These patients were mostly men , had a family history of premature CAD , and 60 % ( 414 of 690 ) had high - density lipoprotein ( HDL ) cholesterol less than 0.90 mmol / liter ( 35 mg / dl ) . - pubmed
In a separate group of patients defined from the same admission population but having angiographically established CAD , 32 % ( 424 of 1,197 ) had a total cholesterol less than 5.17 mmol / liter ( 200 mg / dl ) , 59 % of whom ( 252 of 424 ) had HDL less than 0.90 mmol / liter ( 35 mg / dl ) . - pubmed
An analysis of persons admitted electively for angiography ( to exclude any effects of hospitalization per se on serum lipids ) revealed a similar proportion of persons with total cholesterol less than 5.17 mmol / liter ( 200 mg / dl ) ( 35 % ) , CAD ( 82 % ) , and HDL less than 0.90 mmol / liter ( 35 mg / dl). - pubmed
With straight chain aliphatic aldehydes ( C1 - C6 ) the Km values of the E3 isozyme are identical with those of the E1 isozyme . - pubmed
All isozymes dehydrogenate naturally occurring aldehydes , 5 - imidazoleacetaldehyde ( histamine metabolite ) and acrolein ( product of beta - elimination of oxidized polyamines ) with similar catalytic efficiency . - pubmed
Differences between the isozymes are in the Km values for aminoaldehydes . - pubmed
Although all isozymes can dehydrogenate gamma - aminobutyraldehyde , the Km value of the E3 isozyme is much lower : the same appears to apply to aldehyde metabolites of cadaverine , agmatine , spermidine , and spermine for which Km values range between 2 - 18 microM and kcat values between 0.8 - 1.9 mumol / min / mg . - pubmed
Thus , the E3 isozyme has properties which make it suitable for the metabolism of aminoaldehydes . - pubmed
The physiological role of E1 and E2 isozymes could be in dehydrogenation of aldehyde metabolites of monoamines such as 3,4 - dihydroxyphenylacetaldehyde or 5 - hydroxyindoleacetaldehyde ; the catalytic efficiency with these substrates is better with E1 and E2 isozymes than with E3 isozyme . - pubmed
Although the enzyme is generally considered to function in detoxication , our data suggest an additional function in metabolism of biogenic amines . - pubmed
Mossy fibres form synapses with identified pyramidal basket cells in the CA3 region of the guinea - pig hippocampus : a combined Golgi - electron microscope study . - pubmed
By using the combined Golgi - electron microscope technique mossy fibre terminals were found , which established asymmetric synaptic contacts with both spines of pyramidal cells and smooth dendrites of identified ( Golgi - stained ) pyramidal basket cells in the CA3 region of the guinea - pig hippocampus . - pubmed
A 57 kd component of oligosaccharyl transferase , termed glycosylation site binding protein , specifically recognizes a photoaffinity probe containing the N - glycosylation site sequence Asn - Lys - Thr . - pubmed
Corticotropin - releasing factor ( CRF ) and dynorphin - related peptides are co - localized within a subset of hypothalamic neurons suggesting the possibility of their co - release . - pubmed
Upon simultaneous administration , 0.1 nmol of dynorphin A1 - 17 , but not 0.1 nmol of dynorphin A1 - 8 or dynorphin A1 - 13 , markedly attenuated CRF - induced elevations of AP , HR , and plasma catecholamine levels . - pubmed
The activity changes of lactate dehydrogenase reflected changes in tissue lactate levels and succinate dehydrogenase activity had a tissue specificity . - pubmed
GTP - binding proteins appear to couple receptors to phospholipase - C - mediated PPI breakdown . - pubmed
Fluoride ( 1 - 30 mM ) also inhibited PTH release in dose - dependent fashion . - pubmed
Fluoride ( 30 mM ) produced 72.8 + /- 4.2 % suppression of maximal low Ca2 + -stimulated PTH release comparable to the 83.7 + /- 3.7 % inhibition by 2.0 mM extracellular Ca2 + . - pubmed
Since changes in both [ Ca2+]i and cAMP regulate PTH release , we measured the effect of fluoride on intracellular cAMP . - pubmed
While both may contribute to the fluoride - induced suppression of PTH release , our findings suggest that the stimulation of PPI turnover leads to inhibition of PTH secretion . - pubmed
Phy is metabolized to eseroline and three other metabolites , M1 , M2 , and M3 . - pubmed
The maximum inhibition of BuChE ( 52 % ) correlates with the highest Phy concentration ( 84.6 ng / ml ) in plasma at 2 min and 70 % of the enzymic activity recovered by 45 min . - pubmed
Previous acute studies of intratracheal cannulation ( ITC ) versus intralaryngeal cannulation ( ILC ) showed that tracheal cell proliferation increased significantly in ITC - induced mucosal wounds . - pubmed
Only mild increases in intrapulmonary cell proliferation were produced by Fe2O3 - saline given by ILC or ITC ( Keenan et al . - pubmed
The present chronic studies included the following variables : a single instillation by ILC of N - methyl - N - nitrosourea ( MNU ) at 5 weeks of age ; 15 weekly treatments ( beginning at 7 weeks of age ) by ILC or ITC alone , or with instillations of saline , or Fe2O3 - saline , or BP - Fe2O3 - saline ; and appropriate controls . - pubmed
Our conclusions are as follows : Plasma beta - endorphin shows a circadian rhythm similar to that seen with corticotropin ( ACTH ) and is suppressable by dexamethasone . - pubmed
When abnormality of either the beta - endorphin or cortisol is considered it is clear that both levels of the hypothalamic - pituitary - adrenal axis can be dysregulated in endogenous depression . - pubmed
An improved method is described in which toxic APAP metabolites are generated by a purified and reconstituted cytochrome P-450 system , minimizing the amount of exogenous detoxification enzymes in the assay . - pubmed
Toxicity required cytochrome P-450 and NADPH , and was inhibited by SKF 525A. - pubmed
The data are consistent with the generation of cytochrome P-450 - dependent reactive metabolites which subsequently can be detoxified by glutathione . - pubmed
Exostoses have already been studied and explained by the action of BAPN ( lysyl oxidase ) . - pubmed
Release was determined by assay of biochemical markers ( serotonin for dense granules and beta - thromboglobulin for alpha - granules ) . - pubmed
A tenfold higher concentration of prostacyclin was required to inhibit completely serotonin release as compared to the concentration required for beta - thromboglobulin release . - pubmed
The decrease in toxin - binding ability of synaptosomes was paralleled by a release of sialic acid from the synaptosomes by the neuraminidase treatment . - pubmed
The toxin - binding ability of synaptosomes treated with neuraminidase was lower than untreated ones at a high concentration of sodium chloride . - pubmed
In contrast , 17 alpha - OAc and especially 21 - OAc substitution on hydrocortisone and betamethasone causes a decrease in the steroid affinity for the receptor and an increase in steroid lipophilicity . - pubmed
The elongation of the ester chain from acetate to valerate in both position C-17 and C-21 leads to the increase in both the binding affinity for the receptor and the lipophilicity of steroids . - pubmed
Only in the series of 17 alpha- and 21 - esters is there a correlation between the binding affinity of steroids for the glucocorticoid receptor and their lipophilicity . - pubmed
An investigation of Ca2 + -binding centers of calmodulin was carried out by EXAFS - spectroscopy . - pubmed
The experimental results for protein preparations of calmodulin in which Ca2 + was isomorphically replaced by Tb3 + were obtained by a spectrometer working at the Institute of Nuclear Physics . - pubmed
Coincidence main maxima on phi ( r ) curves and identity of Fourier transformation for calmodulin and parvalbumin in the 2 - 6 A interval allow to infer the identity of Ca2 + -binding centers of calmodulin and parvalbumin . - pubmed
Utilization of pppGpp and dGTP by initiation factor 2 and elongation factor Tu . - pubmed
Histidyl groups seem to be located in the active site of the dicarboxylic transporter in that the simultaneous presence of Na and succinate protects the transporter against the histidyl specific reagent diethylpyrocarbonate . - pubmed
Lithium , which specifically competes for sodium sites in the dicarboxylic acid transporter , substantially blocked the protective effect of Na and succinate . - pubmed
We conclude that a histidyl residue is at , or is close to , the active site of the dicarboxylate transporter in renal brush border membranes . - pubmed
The activities of sialyl- and galactosyltransferases towards endogenous acceptors in the synaptic junction fraction were low but increased greatly after the addition of deglycosylated fetuin or mucin as exogenous acceptors . - pubmed
Hydroperoxides are determined from the coupled activities of glutathione peroxidase and glutathione reductase as measured by the loss of NADPH absorbance . - pubmed
To minimize the effects of tissue catalyzed peroxide degradation , incubations were performed in the presence of 1,3 - bis ( 2 - chloroethyl )- 1 - nitrosourea ( BCNU ) ; which inhibited the activity of retinal tissue glutathione reductase by 85 % . - pubmed
Rat brain transketolase and pyruvate dehydrogenase were inhibited 25 percent by 10 - 9 M moniliformin . - pubmed
Studies carried out to determine if moniliformin causes enzyme inhibition by reaction with thiamine were negative . - pubmed
After treatment with macrolide antibiotics , a significant reduction in the number of lymphocytes and activated CD8 + cells and an elevation in the CD4 / CD8 ratio in BALF of DPB patients was observed . - pubmed
Similarly , the content of alpha - hydroxyaldehydes produced from plasmalogens via their epoxids during lipidperoxidation ( LPO ) was studied . - pubmed
VLDL and LDL plasmalogens were oxidized to a greater extend ( 99 % decrease ) compared to HDL plasmalogens ( 35 % ) . - pubmed
Specificity of the binding of E3 to E2 was certified by a competition experiment measuring binding of biotin - labeled E3 in the presence of unlabeled E3 . - pubmed
Influence of monovalent cations on the ultraviolet - visible spectrum of tryptophan tryptophylquinone - containing methylamine dehydrogenase from bacterium W3A1 . - pubmed
Except for NH4 + , all spectral changes produced by binding of the monovalent cations can be explained by assuming two different binding sites in MADH ( type I and type II sites ) . - pubmed
Na + and K + displayed monophasic binding to the type II site , ( CH3 ) 3NH+ and ( CH3 ) 4N+ displayed monophasic binding to the type I site , and Li + , Rb + , and Cs + displayed either monophasic or biphasic binding to one or both sites depending on pH. - pubmed
CA III activity was significantly increased in SS - treated EDL and TA muscles compared to control ( CTR ) , while SNE injections partially prevented this increase . - pubmed
The CA III mRNA increase observed in response to denervation was reduced by 40 % in SNE - treated EDL and TA muscles . - pubmed
Heart rate ( HR ) , contractility ( dP / dt ) , mean arterial blood pressure ( MABP ) , left ventricular pressure ( LVP ) , coronary sinus pressure ( CSP ) , left - ventricular end - diastolic pressure ( LVEDP ) , coronary blood flow ( CBF ) , coronary vascular resistance ( CVR ) , myocardial oxygen consumption ( MVO2 ) and coronary sinus lactate were determined after 20 min in each position . - pubmed
A common amino acid polymorphism in insulin receptor substrate-1 causes impaired insulin signaling . - pubmed
As compared to wild - type IRS-1 , insulin stimulation of cells transfected with mutant IRS-1 exhibited a 32 % decrease in incorporation of [ 3H ] thymidine into DNA ( P = 0.002 ) , a 36 % decrease in IRS-1 associated phosphatidylinositol ( PI ) 3 - kinase activity ( P = 0.004 ) and a 25 % decrease in binding of the p85 regulatory subunit of PI 3 - kinase to IRS-1 ( P = 0.002 ) . - pubmed
The changes occurred with no change in insulin receptor or IRS-1 tyrosine phosphorylation . - pubmed
Two crystal structures of the leupeptin - trypsin complex . - pubmed
Three - dimensional structures of trypsin with the reversible inhibitor leupeptin have been determined in two different crystal forms . - pubmed
The leupeptin backbone forms four hydrogen bonds with trypsin and a fifth hydrogen bond interaction is mediated by a water molecule . - pubmed
The reaction of trypsin with leupeptin proceeds through the formation of stable tetrahedral complex in which the hemiacetal oxygen atom is pointing out of the oxyanion hole and forming a hydrogen bond with His-57 . - pubmed
lividans ( ard2 ) were shown to phosphorylate A201A in an ATP - dependent reaction . - pubmed
Phosphorylation by Ard2 takes place on the hydroxyl group at C2 of the unsaturated hexofuranose moiety of A201A , as shown by 1H - NMR analysis of purified P - A201A. - pubmed
Since unlike their normal counterparts , triggering of their TCR / CD3 in vitro elicits only a weak mitogenic response , we set out to determine which of the signal transduction molecules initiated by anti - CD3E antibodies are affected in neoplastic cells . - pubmed
The results obtained from analysis of tumor cells from four patients show a general reduction in basal and induced tyrosine phosphorylation of a wide range of signaling proteins . - pubmed
SurP , like other sucrose EIIs , lacked the hydrophilic domain containing the first ( IIA ) phosphorylation site . - pubmed
Cells deleted for rsv1 + are unable to survive glucose starvation . - pubmed
Transcription of rsv1 + is negatively regulated by the cAMP pathway and induced by glucose starvation . - pubmed
The nitric oxide synthase inhibitor N ( G )- nitro - L - arginine , ( 100 microM ) reduced [ 3H ] L - citrulline production in preparations from all three species . - pubmed
Omission of calcium or NADPH significantly reduced nitric oxide synthase - like activity in preparations from all three species but arginase - like activity was not significantly affected . - pubmed
These results provide direct evidence that the two conserved cationic residues Arg33 and Lys27 , located on loop II of the toxin structure , are binding in close proximity to the alpha - subunit region between residues 188 - 200 . - pubmed
This is supported by a number of experimental findings : ( i ) NiR - deficient mutants have lost Nii1 gene expression ; ( ii ) Nii1 mRNA accumulation is co - regulated with the expression of other structural genes of the nitrate assimilation pathway ; ( iii ) nitrite ( nitrate ) utilization ability is recovered in the NiR mutants by functional complementation with a wild - type Nii1 gene ; ( iv ) the elucidated NII1 amino acid sequence is highly similar to that of the cyanobacterial and higher - plant enzyme , and contains the predicted domains for plastidic ferredoxin - NiRs . - pubmed
Lde1 ( light - dependent expression ) , was not regulated by nitrogen , but like nitrate - assimilation clustered genes , its expression was down - regulated in the dark . - pubmed
Inactivation of cytochrome P450 2E1 by tert - butylisothiocyanate . - pubmed
Several naturally occurring and synthethic isothiocyanates were evaluated for their ability to inactivate the major ethanol - inducible hepatic cytochrome P450 2E1 . - pubmed
Of the compounds tested , tert - butylisothiocyanate ( tBITC ) was found to be the most selective inactivator of the 2E1 p - nitrophenol hydroxylation activity . - pubmed
The kinetics of inactivation of P450 2E1 by tBITC were characterized . - pubmed
When cytochrome b5 was co - reconstituted with P450 2E1 , the apparent KI for P450 2E1 inactivation by tBITC was similar to that seen in microsomes ( 14 microM ) . - pubmed
P450 2E1 T303A was also inactivated by tBITC with kinetic constants similar to that of the wild type enzyme . - pubmed
Co - incubations with an alternate substrate protected P450 2E1 from inactivation by tBITC . - pubmed
The extent of P450 2E1 inactivation by tBITC resulted in a comparable loss of the ability of the enzyme to form a reduced CO complex . - pubmed
Although angiotensin receptor antagonists ( AII - A ) share the common property with ACE - I with regard to blockade of angiotensin activity via angiotensin type 1 receptors ( AT1 ) , AII - A is also reported to stimulate AT2 that plausibly activates nitric oxide production within renal medulla and augments synthesis of vasodilatory P450 - metabolites in renal afferent arterioles . - pubmed
In contrast , AII - A is reported to have no effect on bradykinin activity . - pubmed
beta - adrenergic blockade in developing heart failure : effects on myocardial inflammatory cytokines , nitric oxide , and remodeling . - pubmed
BACKGROUND : Whether beta - adrenergic blockade modulates myocardial expression of inflammatory cytokines and nitric oxide ( NO ) in heart failure is unclear . - pubmed
Papillary muscle studies revealed isoproterenol hyporesponsiveness to be unaltered by NO synthase ( NOS ) inhibition . - pubmed
Metoprolol administration starting 48 hours after infarction ( 1 ) attenuated ( P<0.02 ) LV dilatation and systolic dysfunction , ( 2 ) preserved isoproterenol responsiveness ( P<0.025 ) via NO - independent mechanisms , and ( 3 ) reduced myocardial gene expression and protein production of TNF - alpha and IL-1beta ( P<0 . - pubmed
CONCLUSIONS : During heart failure development , adrenergic activation contributes to increased myocardial expression of TNF - alpha and IL-1beta but not IL-6 , and one mechanism underlying the beneficial effects of beta - adrenergic blockade may involve attenuation of TNF - alpha and IL-1beta expression independent of iNOS and NO . - pubmed
The interactions between alcohol and cytochrome P-450 enzymes have been well investigated . - pubmed
However , the data regarding the effect of alcohol on the regulation of UDP - glucuronosyltranferase ( UGT ) activity are less clear . - pubmed
In cultured hepatocytes , treatment with either ethanol or isopentanol significantly increased the expression of UGT1A1 , UGT1A5 , UGT2B1 , and UGT2B3 mRNAs , but to different degrees . - pubmed
The induction of UGT1A1 and UGT2B1 mRNAs by ethanol or isopentanol was time - dependent and maximal changes occurred at 48 h. The expression of UGT1A6 mRNA was not significantly modified by either ethanol or isopentanol . - pubmed
In conclusion , ethanol and isopentanol have direct roles in the regulation of UGT . - pubmed
Lower levels of plasma 11 - ketotestosterone and higher levels of plasma vitellogenin were shown in the E(2 ) group . - pubmed
The method gives good quantitative agreement with standard Soxhlet extraction , and with certified reference materials for PAH concentrations on soil , sediment ( SRM 1944 ) , and air particulate matter ( SRM 1649a ) . - pubmed
Recently , PBSC collection after high - dose cytarabine , with or without G - CSF , has been associated with DFS ranging from 47 to 57 % . - pubmed
In this study we investigated whether oral prednisolone modulates the balance of peripheral blood CXCR3 + and CCR4 + T cells . - pubmed
In the steroid - treated asthma group , there was a decrease in CCR4 + T cells ( from 29.3 % to 20.3 % , p & lt ; 0.0001 ) , and an increase in CXCR3+/ CCR4 + ratio ( from 1.86 to 2.89 , p = 0.0047 ) , whereas there was no change in CXCR3 + T cells . - pubmed
However , the percentages of CCR4 + cells did not change after steroid therapy in normal control subjects . - pubmed
NADH - dependent oxidase activity was shown to be equally induced ( 2- to 3 - fold ) in endothelial cells exposed to pulsatile unidirectional or oscillatory flow patterns . - pubmed
P22phox mRNA level increased in cells exposed to both unidirectional and oscillatory shear stress , suggesting that p22phox gene expression upregulation contributes to flow - induced increase in superoxide anion production in endothelial cells . - pubmed
We reinvestigated the structures of mannobiose ( M(2 ) ) , M(3)s , and mannotetraose ( M(4 ) ) that were completely isolated by matrix - assisted laser desorption ionization time - of - flight mass spectrometry ( MALDI - TOF - MS ) and NMR spectroscopy . - pubmed
cruzi as evidenced by depolarization of the DeltaPsi by H (+)- ATPase inhibitors dicyclohexylcarbo - diimide , N - ethylmaleimide , diethylstilbestrol , and bafilomycin A(1 ) . - pubmed
However , ouabain induced an additional significant depolarization in these stages when their DeltaPsi was already partially depolarized by the H (+)- ATPase inhibitor dicyclohexylcarbo - diimide , supporting the presence of an ouabain - sensitive sodium pump whose activity is masked by the H (+)- ATPase . - pubmed
The presence of K (+ ) channels in amastigotes and a sodium pump in trypomastigotes , in addition to the H (+)- ATPase , could provide important new targets for trypanocidal drug development . - pubmed
We applied two - dimensional gel electrophoresis ( 2 - DE ) to the total exoproteins secreted from pathogenic MRSA strains and identified major protein spots by N - terminal amino acid sequence analysis . - pubmed
Here we find that there is a cholesterol - dependent association between CD45 and the raft - associated protein linker for activation of T cells , suggesting that CD45 and linker for activation of T cells may colocalize in lipid rafts . - pubmed
Consistent with this observation , we find that approximately 5 % of total CD45 can be detected in Triton X-100 - insoluble buoyant fractions of sucrose gradients , demonstrating that CD45 is not excluded from lipid rafts . - pubmed
A His - X - His pseudotripeptide zinc complex ( X is a N - alkyl glycine derivative ) similar to the catalytic center of the carbonic anhydrase was computer designed and experimentally synthesized . - pubmed
Ritonavir and Saquinavir directly stimulate anterior pituitary prolactin secretion , in vitro . - pubmed
An association between human immunodeficiency virus type I ( HIV-1 ) protease inhibitors ( PIs ) and galactorrhoea / hyperprolactinemia adverse effect has recently been reported in four HIV-1 - infected women treated with PIs ( indinavir , nelfinavir , ritonavir or saquinavir ) . - pubmed
This could be explained by a direct effect of ritonavir and saquinavir on anterior pituitary prolactin ( PRL ) release , and/or an indirect effect of PIs on the secretion of hypothalamic dopamine , which is the main PRL inhibitory factor . - pubmed
We found that both ritonavir and saquinavir are able to significantly stimulate PRL secretion , with saquinavir slightly more effective than ritonavir . - pubmed
On the contrary , both protease inhibitors do not modify either basal or depolarization - induced dopamine release . - pubmed
[ Influence of atrazine on the tyrosine environment of catalase ] . - pubmed
By the UV difference spectrum and fluorescence spectrum technique , the influence of atrazine on tyrosine environment of catalase was studied . - pubmed
UV difference spectrum and fluorescence spectrum of atrazine - catalase were red shift that maybe caused by effect of hydrogen bonding . - pubmed
The results indicated that the tyrosine residues of catalase reside in hydrophilic core , and there is hydrogen bonding between atrazine and catalase probably . - pubmed
Atrazine has little effect on tyrosine environment of catalase . - pubmed
The inhibitor LY294002 was able to completely abolish survival mediated by KL , whereas IL-3 and FL were only partially affected . - pubmed
The addition of LY294002 did not affect the expression of Bcl-2 and Bcl - XL , and thus , we conclude that expression of antiapoptotic Bcl-2 family member genes is not dependent on PI-3 kinase activity . - pubmed
Finally , I review recent evidence that the soluble N - ethylmaleimide - sensitive factor attachment protein receptor ( SNARE ) complex , formed between proteins residing in the vesicle and the plasma membrane , is involved in formation and stabilization of the readily releasable vesicle pool , whereas synaptotagmin , a Ca2 + - and phospholipid - binding vesicular protein , is involved in setting the Ca2 + dependence of the fusion process itself . - pubmed
MPS IVA patients had an elevation of urine and plasma keratan sulfate ( KS ) concentrations compared with those of the age - matched control . - pubmed
OBJECTIVE : To study the influence of Cefaclor and Prednisonum to the expression of cell adhesion molecule in experimental otitis media with effusion ( OME ) . - pubmed
Cefaclor and Prednisonum may improve otitis media with effusion , which may related to the decreasing expression of Mac-1 and L - Selectin . - pubmed
AL-6556 and AL-6598 had an affinity ( Ki ) of 2.66 - 4.43 microM for DP receptors but a much lower affinity ( K(i)s = 38 - 103 microM ) for EP3 , FP , IP , and TP receptors ( n = 3 - 5 ) . - pubmed
In addition , AL-6556 and AL-6598 exhibited K(i)s & gt ; 100 microM for 19 nonprostanoid receptors . - pubmed
The DP antagonist , BWA868C , effectively antagonized the effects of AL-6556 with a high potency ( IC50 = 22.8 + /- 3.9 nM ; n = 3 ) . - pubmed
EP2 receptors labeled with [ 3H ] PGE2 were concentrated in the LCM , CM , RET , and iris . - pubmed
In conclusion , AL-6598 and AL-6556 are relatively DP - receptor - selective PGs with full agonist activity at the DP and partial agonist activity at the EP2 receptor . - pubmed
Neutrophil activation was influenced neither by 0.125 % human serum albumin ( HSA ) alone nor by vesicles of F6H8 or silicone oil prepared with 0.125 % HSA . - pubmed
From our previous results , inhibition of various polymerases by 8 - OH - G increases in the following order : Poleta & lt ; Polkappa & lt ; Polbeta & lt ; Polalpha , showing that major replicative and repair polymerases are more sensitive to this lesion than enzymes belonging to the Y - family . - pubmed
In the direct mutagenesis experiments , Polkappa was found to be more mutagenic than Poleta studied previously : it inserted dAMP more efficiently than dCMP opposite 8 - OH - G. - pubmed
Two adjacent 8 - OH - G residues in codon 12 inhibited Polkappa moderately and induced misincorporation of dAMP . - pubmed
A 78 - amino acid insertion ( L78 ) is found in the low - affinity type ( L - form ) of starch phosphorylase ( L - SP , EC 2.4.1.1 ) . - pubmed
However , H - SP ( the high - affinity isomer of SP lacking the L78 insertion ) and the proteolytic modified L - SP , which lost its L78 fragment , could not be phosphorylated . - pubmed
Furthermore , using L78P mutants by site - directed mutagenesis at Ser residues on L78 , we demonstrate that only one Ser residue on L78 is phosphorylated by the kinase . - pubmed
These results imply that this kinase is specific to L - SP , or more precisely , to the L78 insertion . - pubmed
Chemical integrity of the lesion containing oligonucleotides was probed using phosphodiesterase lability . - pubmed
We have also studied whether selenomethionine can modify the effect of overexpression of alpha - synuclein during differentiation of NB cells . - pubmed
We have utilized RO20 - 1724 , an inhibitor of cyclic nucleotide phosphodiesterase , and prostaglandin A1 ( PGA1 ) , a stimulator of adenylate cyclase , or RO20 - 1724 and dibutyryl cAMP to induce terminal differentiation in over 95 % of the cell population by elevating the intracellular levels of cAMP in NB cells . - pubmed
The viability of cells was determined by MTT assay and LDH leakage assay , and the degeneration was documented by photomicrographs . - pubmed
When selenomethionine was added to NB cells overexpressing alpha - synuclein immediately after the addition of RO20 - 1724 and PGA1 , the viability and degenerative changes were markedly reduced , suggesting the involvement of increased oxidative stress in the mechanism of action of alpha - synuclein . - pubmed
Orientation of 6 - mercaptopurine SAMs at the silver electrode as studied by Raman mapping and in situ SERS . - pubmed
Stability of 6MP SAMs was observed by in situ SERS spectroelectrochemical measurements . - pubmed
The difference between the binding of water and methanol is that the water molecule forms a hydrogen bond to O5 of pyrroloquinoline quinone , which the methanol , by virtue of its methyl group , does not . - pubmed
The results support the proposal that aspartate rather than glutamate is the catalytically active base for a hydride transfer mechanism in quinoprotein alcohol dehydrogenases . - pubmed
1,4 - Dihydroisonicotinic acid derivatives ( 1,4 - DHINA ) are compounds closely related to derivatives of 1,4 - dihydropyridine , a well - known calcium channel antagonists . - pubmed
Third - generation cephalosporins and beta - lactam / beta - lactamase inhibitor antibacterials as well as clindamycin or metronidazole are often required to provide broad - spectrum coverage for polymicrobial infections . - pubmed
However , unlike the green tissues , white tissues had no detectable level of galactinol synthase activity and a low level of sucrose phosphate synthase indicating that stachyose and possibly sucrose present in white tissues may have originated in green tissues . - pubmed
Both tissues contained high levels of invertase , sucrose synthase and alpha - galactosidase and extensively metabolized exogenously supplied ( 14 ) C - sugars . - pubmed
Putative raft markers were found to be dynamically compartmented within tens of milliseconds into small microdomains ( O & lt;120 nm ) that are sensitive to the cholesterol and sphingomyelin levels , whereas actin - based cytoskeleton barriers are responsible for the confinement of the transferrin receptor protein . - pubmed
We measured lesion size , macrophage , smooth muscle cell , and collagen accumulation at the aortic sinus , and expression of genes that regulate cholesterol transport and inflammation . - pubmed
In peritoneal macrophages , PPAR - gamma and ABCA1 expression was also reduced by 50 % and 70 % , respectively , as was cholesterol efflux , by 50 % . - pubmed
We suggest this protective role is mediated by PPAR - gamma and ABCA1 , molecules that affect cholesterol homeostasis and inflammation . - pubmed
Three open reading frames encoding peptides larger than 50 amino acids were also identified : repB , encoding a replicase of 315 amino acids , a transcriptionally coupled gene ( orfX - like ) , similar to the orfX of some theta - replicating lactococcal plasmids , and copG - like in the complementary strand , which showed a conserved domain present in proteins of the CopG family . - pubmed
Furthermore , our data indicate that mitoK(ATP ) , in addition to UCPs , may be involved in the control of energy metabolism and body weight . - pubmed
Losartan preserves integrity of cardiac gap junctions and PGC-1 alpha gene expression and prevents cellular apoptosis in remote area of left ventricular myocardium following acute myocardial infarction . - pubmed
This study tests the hypothesis that peroxisome proliferator activated receptor - gamma coactivator 1alpha ( PGC-1alpha ) and the integrity of gap junctions ( GJs ) were suppressed and the number of apoptotic bodies was increased in remote viable areas of left ventricle following acute myocardial infarction ( AMI ) , which can be reversed by losartan therapy . - pubmed
These compounds have been characterized by elemental analyses , FTIR , ESI - MS , and multinuclear ( 1H , 19F , and 31P ) NMR spectroscopy . - pubmed
Several recent studies have confirmed the efficacy and safety of both inhaled corticosteroids and leukotriene receptor antagonists in the management of early childhood asthma . - pubmed
Based on the current body of evidence , there is rationale for further investigation of these management strategies , including direct comparisons between inhaled corticosteroids and leukotriene receptor antagonists , as well as the role of long - acting beta - agonists , potentially targeting the subpopulations of early childhood with wheezing who are at highest risk for persistence of asthma symptoms . - pubmed
The results indicate that psychotic disorders are associated with elevated baseline and challenge - induced HPA activity , that antipsychotic medications reduce HPA activation , and that agents that augment stress hormone ( cortisol ) release exacerbate psychotic symptoms . - pubmed
The football match induced decreased neutrophil vitamin E levels and catalase and glutathione peroxidase activities but increased glutathione reductase activity . - pubmed
These enzymes are characterized by the presence of a conserved helicase domain with specific helicases motifs whose amino acid sequence allows the differentiation between DEAD and DExH - box RNA helicase families . - pubmed
The consideration of the observed thermodynamic parameters suggested that the increased rigidity of the MOR - modified TFO in the free state relative to the corresponding unmodified TFO may enable the significant increase in the binding constant . - pubmed
Analysis of melamine in milk powder by CZE using UV detection and hyphenation with ESI quadrupole / TOF MS detection . - pubmed
An easy to employ method for the determination of melamine in milk powder samples using CZE with either UV or MS detection is presented . - pubmed
Before analysis , milk powder samples were dissolved in water , de - fatted with dichloromethane and diluted with ACN to precipitate the protein , filtrated and injected without further pretreatment . - pubmed
The developed CZE method allowed the determination of melamine in milk powder down to a level of 0.50 mg / kg using UV detection and 0.25 mg / kg when ESI quadrupole / TOF MS was employed for detection . - pubmed
The inner diameter of vessels was recorded by a computerized diameter tracking system and the effects of K (+ ) channels on the development of vascular myogenic tone was tested by application of various K (+ ) channel inhibitors . - pubmed
Characterization of the nebulized tacrolimus dispersion for nebulization showed a fine particle fraction ( FPF ) of 46.1 % and a mass median aerodynamic diameter ( MMAD ) of 4.06 microm . - pubmed
A 20 - amino - acid tandem repeat that forms the core protein of MUC1 is overexpressed and aberrantly glycosylated in the majority of epithelial tumors . - pubmed
AS1402 is a potent inducer of antibody - dependent cellular cytotoxicity ( ADCC ) , specifically against MUC1 - expressing tumor cells . - pubmed
Letter by Choi et al regarding article , " Effects of the selective estrogen receptor modulator raloxifene on coronary outcomes in the raloxifene use for the heart trial : results of subgroup analyses by age and other factors " . - pubmed
Consistent with mediation by D ( 1 )- type receptors , SCH-23390 [ R -(+)- 7 - chloro-8 - hydroxy-3 - methyl-1 - phenyl-2,3,4,5 - tetrahydro-1H-3 - benzazepine ] reversed the effects of dopamine on spikes . - pubmed
Three - dimensional ( 3D ) visualization of endolymphatic hydrops after intratympanic injection of Gd - DTPA : optimization of a 3D - real inversion - recovery turbo spin - echo ( TSE ) sequence and application of a 32 - channel head coil at 3T. - pubmed
PURPOSE : To enable volume visualization of endolymphatic hydrops of Meniere ' s disease via a volume rendering ( VR ) technique , a three - dimensional ( 3D ) inversion - recovery ( IR ) sequence with real reconstruction ( 3D - real IR ) sequence after intratympanic injection of Gd - DTPA was optimized for higher spatial resolution using a 32 - channel head coil at 3T. - pubmed
Examining precursors from the purest white adipose tissue depot ( epididymal ) , we report here that chronic treatment with the peroxisome proliferator - activated receptor gamma agonist rosiglitazone promotes not only the expression of PGC-1alpha and mitochondriogenesis in these cells but also a norepinephrine - augmentable UCP1 gene expression in a significant subset of the cells , providing these cells with a genuine thermogenic capacity . - pubmed
DNase activity was measured by DNase agar clearance with and without Methyl Green . - pubmed
Neon shows continuous distribution throughout the channel pore in AQP1 as is the case of water , although the PMF of Ne at the selective filter ( SF ) region is higher than that of water , indicating that the stability of molecules in the channel is determined not only by their size , but also by the charge distribution . - pubmed
The ligand molecules , CO ( 2 ) , NO , urea , and glycerol , have a large barrier in PMF at the SF region in AQP1 , indicating that the channel is not permeable by those ligands . - pubmed
Therefore , our theory predicts that a NH ( 3 ) molecule can be permeated through the AQP1 channel . - pubmed
In GlpF , all the ligands have negative PMF throughout the channel pore except for glycerol , which has a small barrier at the SF area , approximately 2.1 kJ / mol . - pubmed
Also , high - density lipoprotein ( HDL ) cholesterol in males and triglyceride in females was significantly correlated with the IPSS total score . - pubmed
The gene fused to NUP98 ( nucleoporin 98 ) was detected by comparative genomic hybridization array as the retinoid acid receptor gamma gene ( RARG ) . - pubmed
Glutamate released from the presynaptic component activates postsynaptic glutamate receptors that are anchored in the postsynaptic density ( PSD ) and induces subsequent molecular events in the postsynaptic cytoplasm . - pubmed
Liver marker enzymes such as serum alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) and serum albumin ( ALB ) , total protein ( TP ) , total bilirubin ( TB ) and direct bilirubin ( DB ) levels and the markers for oxidative defense namely malondialdehyde ( MDA ) , reduced glutathione ( GSH ) , total thiols ( TT ) and catalase ( CAT ) were measured . - pubmed
Treatment with MEAC ( 200 and 400 mg / kg ) significantly prevented the rise of MDA and TP levels ( P<0.01 ) , and prevented the reduction of GSH , CAT and TT levels significantly compared with PCM - treated group ( P<0.01 ) . - pubmed
An improved Abbott ARCHITECT assay for the detection of hepatitis B virus surface antigen ( HBsAg ) . - pubmed
Captured antigen is then detected using anti - HBsAg antibody conjugated with the chemiluminescent compound , acridinium . - pubmed
Production of novel antioxidative prenyl naphthalen - ols by combinational bioconversion with dioxygenase PhnA1A2A3A4 and prenyltransferase NphB or SCO7190 . - pubmed
2 -( 2,3 - Dihydroxyphenyl )- 5,7 - dihydroxy - chromen-4 - one ( 2 ' , 3 '- dihydroxychrysin ) generated with another aromatic dihydroxylating dioxygenase and subsequent dehydrogenase was also geranylated at the C-5'-carbon by the action of NphB. - pubmed
Towards the discovery of a novel class of monoamine oxidase inhibitors : structure - property - activity and docking studies on chromone amides . - pubmed
This sample of phenytoin users showed no drug related adverse reactions and CYP2C9 allele type correlation . - pubmed
The role of CYP2C9 polymorphism influence on phenytoin adverse reaction remains to be determined since some literature evidence and our data found negative results . - pubmed
Specifically , the probe consists of a superparamagnetic iron oxide nanoparticle core ( IONP ) decorated with satellite CdS : Mn / ZnS Qdots where the Qdots themselves are further functionalized with STAT3 inhibitor ( an anti - cancer agent ) , vitamin folate ( as targeting motif ) and m - polyethylene glycol ( mPEG , a hydrophilic dispersing agent ) . - pubmed
The Qdot luminescence is quenched in this nanocomposite probe ( " OFF " state ) due to combined electron / energy transfer mediated quenching processes involving IONP , folate and STAT3 agents . - pubmed
METHOD : Hepatic function in the immunological liver injury model was evaluated by assessing the levels of ALT in plasma , and the content of MDA , ORAC , NO and iNOS mRNA in liver tissues . - pubmed
RECENT FINDINGS : The gene SLC2A9 encodes for GLUT9 , an important proximal tubule transporter of uric acid . - pubmed
Genetic studies of urate transportation and of uromodulin - related nephropathy emphasize the pivotal importance of the proximal tubule in uric acid homeostasis . - pubmed
SUMMARY : Renal tubular handling of uric acid is dependent on tubular transporters , one of which is GLUT9 . - pubmed
Mutations of its gene SLC2A9 are associated with aberrations of uric acid disposal . - pubmed
OBJECTIVE : To perform a retrospective analysis of UFT and oral leucovorin plus PSK combination adjuvant chemotherapy for stage III colon cancer in order to evaluate both treatment efficacy and toxicity . - pubmed
As well as lower meristem cell activity , the second reason for the Se - induced growth hindrance is the hormonal imbalance , since the in situ expression of the auxin - responsive DR5::GUS , and consequently the auxin levels , significantly decreased , while that of the cytokinin - inducible ARR5::GUS and the ethylene biosynthetic ACS8::GUS increased . - pubmed
High levels of NO in gsnor1 - 3 , lead to selenite tolerance , while low NO production in nia1nia2 resulted in selenite sensitivity . - pubmed
Dyslipidemia , and especially atherogenic dyslipidemia , a combination of small low - density lipoproteins cholesterol ( LDL - C ) , decreased high - density lipoprotein cholesterol ( HDL - C ) and increased triglyceride ( TG ) concentrations , represents a major cardiovascular ( CV ) risk factor . - pubmed
This study aims to determine whether hormone therapy ( HT ) for 1 year can modulate adrenal androgen production . - pubmed
A 34 - point AIS - APS was developed from the set of independent predictors including age , history of atrial fibrillation , congestive heart failure , chronic obstructive pulmonary disease and current smoking , prestroke dependence , dysphagia , admission National Institutes of Health Stroke Scale score , Glasgow Coma Scale score , stroke subtype ( Oxfordshire ) , and blood glucose . - pubmed
Plasma total cholesterol concentrations were significantly lower in the XD group than in the CD group ( p & lt ; 0.05 ) . - pubmed
Urinary amounts of equol were significantly higher in the XD group than in the CD group ( p & lt ; 0.05 ) . - pubmed
MGM-16 exhibited a high affinity for mu - and delta - opioid receptors , with K(i ) values of 2.1 and 7.0 nM , respectively . - pubmed
Use of CO as metabolic fuel for microbes relies on enzymes like carbon monoxide dehydrogenase ( CODH ) and acetyl - CoA synthase ( ACS ) , which catalyze conversions resembling processes that eventually initiated the dawn of life . - pubmed
CODHs catalyze the ( reversible ) oxidation of CO with water to CO2 and come in two different flavors with unprecedented active site architectures . - pubmed
Ni- and Fe - containing CODHs are frequently associated with ACS , where the CODH component reduces CO2 to CO and ACS condenses CO with a methyl group and CoA to acetyl - CoA.Our current state of knowledge on how the three enzymes catalyze these reactions will be summarized and the different strategies of CODHs to achieve the same task within different active site architectures compared . - pubmed
PLD generates phosphatidic acid by catalyzing the hydrolysis of phosphatidylcholine ( PtdCho ) and in some systems this PtdOH is de - phosphorylated to generate DAG . - pubmed
In contrast , DGK catalyzes the phosphorylation of DAG thereby converting it into PtdOH . - pubmed
While both enzymes are poised to regulate the levels of DAG and PtdOH , therefore , they both lead to the generation of PtdOH and could have opposite effects on DAG levels . - pubmed
In this report , we review the recent understanding of the roles PLDs , DGKs , and DAG lipases , as well as sphingolipids and cholesterol play in synaptic vesicle cycling . - pubmed
Cell cycle analysis suggested that CUR micelles induced apoptosis and enhanced G2 / M arrest . - pubmed
MAIN MEASURES : Use of advanced imaging ( CT / MRI ) , opioids / barbiturates , and referrals to other physicians ( guideline - discordant indicators ) , as well as counseling on lifestyle modifications and use of preventive medications including verapamil , topiramate , amitriptyline , or propranolol ( guideline - concordant during study period ) . - pubmed
Only herd A and B strains harboured the genes for beta - lactams resistance ; the leukocidin D / E gene , a type I site - specific deoxyribonuclease subunit , 3rd locus gene and serin - protease A and B were carried by all strains , but not by V329 , while serin - protease E was absent in V329 and in another isolate . - pubmed
Here it is described how photon - in photon - out hard X - ray spectroscopy techniques can be applied to study the electronic structure and its dynamics of transition metal systems with ultra - bright and ultra - short FEL X - ray pulses . - pubmed
Postoperative CD36 values were significantly correlated with total aneurysm volume ( p = 0.05 ) and were higher in endografts made from polyester ( p = 0.01 ) . - pubmed
Superoxide scavengers were not radioprotective in this paradigm , indicating the mechanisms for both loss of clonogenicity and PB1 radioprotection are independent of superoxide signaling . - pubmed
In contrast 1 , 25 ( OH ) 2D3 had no significant effect upon the expression of either TRPV 5 or 6 at either the protein or the mRNA level . - pubmed
Para - amino acetanilide biodegradation was assessed using spectrophotometry and confirmed by gas chromatography - mass spectroscopy ( GC - MS ) . - pubmed
According to GC - MS results , TBZ3 degrades PAA to benzene , hexyl butanoate , 3 - methyl-1 - heptanol and hexyl hexanoate . - pubmed
CONCLUSIONS : LMWH bridging therapy significantly increases the risk of pacemaker pocket hematoma and hemorrhage , and the pacemaker implantation may be safer after DES implantation 6 months . - pubmed
RESULTS : Cuprizone caused loss of myelin basic protein ( MBP ) immunofluorescence in the CC suggesting demyelination . - pubmed
Cuprizone increased the density of CD45+/Iba1 + microglial cells and also increased Gal-3 expression in the CC . - pubmed
Surprisingly , the number of Gal-3 + and CD45 + cells decreased in the SVZ after cuprizone , suggesting inflammation was selectively reduced therein . - pubmed
Inflammation can regulate SVZ proliferation and indeed the number of phosphohistone H3 + ( PHi3 + ) cells decreased in the SVZ but increased in the CC in both genotypes after cuprizone treatment . - pubmed
CONCLUSIONS : This study reveals a uniquely regulated inflammatory response in the SVZ and shows that Gal-3 participates in remyelination in the cuprizone model . - pubmed
Here , we report on the hybrid hole transport materials 4,4 '- bis -( carbazole-9 - yl ) biphenyl ( CBP ) or poly - N - vinylcarbazole ( PVK ) doped into poly ( 4 - butyl - phenyl - diphenyl - amine ) ( Poly - TPD ) as the hybrid hole transport layer ( HTL ) to tailor the energy band alignment between hole injection layer ( HIL ) and quantum dot ( QD ) light emitting layer in order to realize efficient quantum dot light emitting diodes ( QLEDs ) in all solution - processed fabrication . - pubmed
Compared to the pristine Poly - TPD based device , it is found that the electroluminescence ( EL ) performance of QLEDs can be significantly improved by 1.5 fold via addition of CBP into Poly - TPD , which can be attributed to the lowered highest occupied molecular orbital ( HOMO ) level of Poly - TPD to reduce the energy barrier between HTL and valance band ( VB ) of QDs . - pubmed
The interactions between DNA and HMGB1 are defined by hydrogen bonds and van der Waals contacts . - pubmed
Cytochrome P450 - 2D6 Genotype Definition May Improve Therapy for Paroxysmal Atrial Fibrillation A Case of Syncope Following " Pill - in - the - Pocket " Quinidine plus Propafenone . - pubmed
The results demonstrated that some flavonoids including scutellarein , luteolin , baicalein , quercetin and scutellarin displayed strong to moderate inhibitory effects against EcGUS , with the IC50 values ranging from 5.76 muM to 29.64 muM , while isoflavones and dihydroflavones displayed weak inhibitory effects against EcGUS . - pubmed
Further investigation on inhibition kinetics revealed that scutellarein and luteolin functioned as potent competitive inhibitors against EcGUS - mediated PNPG hydrolysis , with the Ki values less than 3.0 muM. - pubmed
Molecular docking simulations demonstrated that scutellarein and luteolin could be well - docked into the catalytic site of EcGUS , while the binding areas of these two natural inhibitors on EcGUS were highly overlapped with that of PNPG on EcGUS . - pubmed
Additionally , the structure - inhibition relationships of natural flavonoids against EcGUS are also summarized , which will be very helpful for the medicinal chemists to design and develop more potent flavonoid - type inhibitors against EcGUS . - pubmed
We found that these plastids have lost isopentenyl pyrophosphate biosynthesis and ribulose-1,5 - bisphosphate carboxylase / oxygenase - based carbon fixation but have retained various proteins for other metabolic pathways , including amino acid biosynthesis , and a portion of the Calvin - Benson cycle comprised only of glycolysis / gluconeogenesis and the reductive pentose phosphate pathway ( rPPP ) . - pubmed
NIES-3581 and the presence / absence of plastid sugar phosphate transporters , we propose that plastid proteins for glycolysis , gluconeogenesis , and rPPP are retained even after the loss of photosynthesis because they feed indispensable substrates to the amino acid biosynthesis pathways of the plastid . - pubmed
Given the correlated retention of the enzymes for plastid glycolysis , gluconeogenesis , and rPPP and those for plastid amino acid biosynthesis pathways in distantly related nonphotosynthetic plastids and cyanobacteria , we suggest that this substrate - level link with plastid amino acid biosynthesis is a key constraint against loss of the plastid glycolysis / gluconeogenesis and rPPP proteins in multiple independent lineages of nonphotosynthetic algae / plants . - pubmed
The increase in Runx2 and ALP activity was accompanied by the activation of calcium / calmodulin - dependent protein kinase type II ( CaMK II ) and extracellular signal - regulated kinases 1 / 2 ( ERK1 / 2 ) , which was completely abolished by treatment with KN93 and U0126 , respectively . - pubmed
Mannosylcalix [ n ] arenes as multivalent ligands for DC - SIGN . - pubmed
DC - SIGN is involved in the recognition process in the form of a tetramer and the multiple exposition of carbohydrate recognition sites ( CRSs ) is amplified by the formation on the DCs membrane of patches of tetramers . - pubmed
We herein prepared four mannosylated calix [ n ] arenes ( 1a - d ) and tested them by Surface Plasmon Resonance ( SPR ) competition assays as inhibitors of the binding between DC - SIGN and a mannosylated BSA used as model of HIV gp120 . - pubmed
Chronic macrolide treatment could possibly select resistant NTM strains and so could make the treatment clearly more difficult as macrolides are the cornerstone NTM treatment . - pubmed
Polyvinylpyrrolidone coated silver nanoparticles ( PVP - AgNPs ) were applied at different concentrations to reduce total nitrogen ( TN ) losses and the mechanisms of nitrogen bio - transformation were investigated in terms of the nitrogen functional enzymes and genes . - pubmed
In this regard , glial cells express cannabinoid receptors and synthesize endocannabinoids , and cannabinoid drugs affect some functions of these cells that can be implicated in schizophrenia pathobiology . - pubmed
Acetyl - CoA promotes glioblastoma cell adhesion and migration through Ca2 + -NFAT signaling . - pubmed
However , the specific molecular mechanisms through which acetyl - CoA production impacts gene expression and its functional roles in promoting malignant phenotypes are poorly understood . - pubmed
Here , using histone H3 Lys27 acetylation ( H3K27ac ) ChIP - seq ( chromatin immunoprecipitation [ ChIP ] coupled with next - generation sequencing ) with normalization to an exogenous reference genome ( ChIP - Rx ) , we found that changes in acetyl - CoA abundance trigger site - specific regulation of H3K27ac , correlating with gene expression as opposed to uniformly modulating this mark at all genes . - pubmed
Genes involved in integrin signaling and cell adhesion were identified as acetyl - CoA - responsive in glioblastoma cells , and we demonstrate that ATP citrate lyase ( ACLY)-dependent acetyl - CoA production promotes cell migration and adhesion to the extracellular matrix . - pubmed
Mechanistically , the transcription factor NFAT1 ( nuclear factor of activated T cells 1 ) was found to mediate acetyl - CoA - dependent gene regulation and cell adhesion . - pubmed
This occurs through modulation of Ca2 + signals , triggering NFAT1 nuclear translocation when acetyl - CoA is abundant . - pubmed
The findings of this study thus establish that acetyl - CoA impacts H3K27ac at specific loci , correlating with gene expression , and that expression of cell adhesion genes are driven by acetyl - CoA in part through activation of Ca2 + -NFAT signaling . - pubmed
CONCLUSIONS : For CHR females , the effect of CAT on adult SUI is mediated via depression , while for males , CAT and its domains have mainly direct effects in maintaining SUI . - pubmed
Some GABAergic terminals are negatively influenced by 5 - HT , suggesting that the hyperphagic effect of CB1R activation could involve changes in serotonergic and GABAergic signaling in the PVN . - pubmed
Here , we have found that the neurochemical mechanisms activated by stimulation of CB1 receptors in the PVN involve inhibition of 5 - HT release , resulting in a decrease of serotonergic activity mediated by 5 - HT1A and 5 - HT1B receptors and inducing disinhibition of GABA release to stimulate food intake . - pubmed
These lysins showed rapid killing kinetics and were active in the presence of high concentrations of salt and urea and under pH conditions ranging from 5.0 to 10.0 . - pubmed
Each model was evaluated for weight gain , fat mass , fasting plasma glucose , insulin and C - peptide , insulin sensitivity , glucose tolerance , lipid profile and liver lipid deposition , blood pressure , and autonomic nervous system function . - pubmed
Samples were divided into control ( Group I - normal saline , Group II-17 % ethylenediaminetetraacetic acid ) and experimental ( Group III , IV , V , VI , VII , VIII , and IX ) groups based on the type of final rinse solution ( 5 mL ) used , that is , TA - premixed ( P ) , TA-(P)-sonic , TA-(P)-ultrasonic , 3 % TA solution , 5 % TA solution , 10 % TA solution , and 10 % citric acid . - pubmed
Transglutaminases ( TGs ) are Ca2 + - and thiol - dependent acyl transferases and protein crosslinking enzymes . - pubmed
Post - annotation , we randomly selected a set of hundred proteins and computed the percentage distribution of 12 amino acids which have been substantially studied for their role in thermostability . - pubmed
Subsequently , the utility of this HIC - MS method was demonstrated in three different applications , where a mAb mixture , mAb molecular variants resulting from PTMs , and a Cys - based ADC mimic were all readily characterized in detail . - pubmed
Also , vitaminD reduces the production of inflammatory cytokines , such as IL-2 and interferon - gamma ( INF - gamma ) . - pubmed
Of interest , another mechanistic approach responds to considering the inhibition of the renin - angiotensin - aldosterone system ( RAAS ) , which is exacerbated in COVID-19 infection because the virus binds to the enzyme ACE2 , making more angiotensinII available to cause damage . - pubmed
VitaminD inhibits mediators of RAAS - present in all cells of the body - and by inhibiting ACE activity and increasing ACE2 , it lowers angiotensinII levels . - pubmed
This study investigated the effect of AT pretreatment of spectinomycin mycelial residues ( SMRs ) for promoting anaerobic biodegradability along with the reduction of antibiotic resistance genes ( ARGs ) , and thus obtained the optimal conditions of AT pretreatment . - pubmed
Biomethane potential ( BMP ) test was conducted to evaluate the anaerobic biodegradability of untreated and pretreated SMRs , and the fate of ARGs was tracked by quantitative polymerase chain reaction . - pubmed
5 - Hydroxymethyl cytosine , enhancer of zeste homolog 2 , cyclin D1 , and programmed death - ligand 1 IHC , and NF2 FISH are all potentially useful but need further study . - pubmed
Emodin enhances cisplatin sensitivity in non - small cell lung cancer through Pgp downregulation . - pubmed
Significant differences are found in the digestion pattern of the two tRNAs using the guanosine - specific Tl nuclease . - pubmed
In carrying out these analyses , the tRNA molecules are labeled either by putting 32P at the 5'-end of the molecular or by adding 32P - labeled pCp at the 3'-end . - pubmed
coli tRNA2Glu have modified Tl nuclease digestion patterns when pCp is added at the 3'-end of the molecule . - pubmed
Evidence is reviewed that 5 - HT ( 5 - hydroxytryptamine , serotonin ) acting through the 5 - HT3 receptor subtype can influence behaviour relevant to anxiety , schizophrenia and cognitive disorders , and that 5 - HT3 receptor antagonists such as ondansetron ( CAS 116002 - 70 - 1 ) can correct behavioural disturbance in the absence of effect on normal behaviour . - pubmed
In the active stage of UC , mucosal spermidine concentration was higher than in remission or in the controls , but the activities of ornithine decarboxylase and S - adenosylmethionine decarboxylase , rate - limiting enzymes of polyamine biosynthesis , were lower . - pubmed
The activity of spermidine / spermine N1 - acetyltransferase , the rate - limiting enzyme of polyamine degradation , was higher in the active stage of UC than in the remission stage of UC or in the controls . - pubmed
Electron microscopy using peroxidase antiperoxidase reveals that tenascin is present in nonstriated , 10 nm wide fibrils and in interstitial bodies , both of which have previously been reported to contain fibronectin . - pubmed
The discussion emphasizes the possible importance of type I collagen in neural crest cell migration and compares the ultrastructural associations of the ECM molecules present at this early embryonic stage . - pubmed
SSAO activity towards dopamine was present in the choroid and the retina , but not in the iris or the optic nerve . - pubmed
The absence of detectable SSAO activity towards dopamine in these last two tissues contrasts with the presence of that enzyme when benzylamine was studied as substrate . - pubmed
Light - induced Ca2 + release from the Ca2 + complex of Nitr-5 altered the FTIR spectra of sarcoplasmic reticulum vesicles and purified Ca ( 2 +)- ATPase preparations . - pubmed
Even though MHC polymorphism has long been known to affect both peptide binding and recognition by the TCR , the role of individual amino acids of MHC proteins in these interactions is poorly understood . - pubmed
Differences in binding of a set of HA307 - 319 analogues biotinylated at each residue to cells expressing DR1 Dw1 and DR1 Dw20 suggested that the polymorphism affected the interactions of many peptide residues with the class II molecule . - pubmed
In inhibition assays , DR1 Dw1 and DR4 Dw4 were shown to differ from DR1 Dw20 and DR4 Dw14 in their length requirements for peptide binding . - pubmed
The results indicated that polymorphism at residue 86 influenced the specificity and affinity of peptide binding and affected the conformation of peptide - DR protein complexes without completely eliminating T cell recognition . - pubmed
Its amino acid composition is similar to that of " tuft " proteins ( enamel matrix protein fragments remaining in the mature tissue ) . - pubmed
Effects of nerve agent antidote and heat exposure on soldier performance in the BDU and MOPP - IV ensembles . - pubmed
This study assessed the effects of nerve agent antidote ( atropine / 2 - PAM chloride versus saline placebo ) and heat - humidity ( 95 degrees F/60 % RH versus 70 degrees F/30 % RH ) on repeated performance of militarily relevant psychological tasks while wearing the battle dress uniform ( BDU ) and while wearing chemical protective clothing ( MOPP - IV ) . - pubmed
MOPP - IV heat sessions could not be continued beyond 2 hours ; all tasks were impaired and subjective reactions were numerous and severe . - pubmed
Atropine/2 - PAM significantly shortened endurance time for heat sessions in MOPP - IV . - pubmed
We measured mean arterial and central venous pressure , heart rate , arterial lactate , blood volume ( 51Cr - labeled red cells ) , hematocrit ( conductivity cell ) , macromolecular extravasation ( 125I - HSA ) and fluid retention ( calculated from change in plasma volume ) . - pubmed
In the presence of alcohols the enzyme catalyses the transfer of the substrate acyl group to alcohols in parallel to hydrolysis . - pubmed
On the basis of titration with diethyl p - nitrophenyl phosphate , a number of four active centres is determined for the tetrameric carboxylesterase . - pubmed
The sequence of 20 amino acid residues at the esterase N - terminus and the amino acid composition are reported . - pubmed
The responses to CCK and BBS were similar in shape , time course , and reversal potential to that induced by receptor mediated phospholipid breakdown and that which is induced by intracellular injection of IP3 . - pubmed
After administration of zimeldine in combination with 5 - HTP there was an increase in synchronized waking ( W-2 ) , followed by an increase in slow wave sleep ( SWS ) , at first SWS-1 with spindles and then highly synchronized SWS-2 . - pubmed
Maximal responses to milrinone and parathyroid hormone were comparable . - pubmed
We find similarities between milrinone and thyroxine actions on bone , because prostaglandin production was crucial for the effects of both agents in calvaria but not in limb bones , and neither agent exhibited escape from calcitonin inhibition . - pubmed
These mutants exhibited a Nif- phenotype in the presence of vanadium , unlike CA12 which was Nif + on vanadium - containing medium . - pubmed
The gene in the cloned nifH - like region is therefore apparently involved in the vanadium dependent alternative pathway of nitrogen fixation . - pubmed
This gene , nifH2 , has been sequenced and encodes a protein of 289 amino acids that is similar to nifH in nucleotide sequence , deduced amino acid sequence , predicted secondary structure and hydrophobicity profile . - pubmed
Molecular analysis of HLA class I and class II antigen loss mutants reveals a homozygous deletion of the DR , DQ , and part of the DP region : implications for class II gene order . - pubmed
DZ alpha genomic sequences were deleted as were the DP alpha 1 and DP beta 1 loci but the DP beta 2 and most , if not all , of the DP alpha 2 locus were retained . - pubmed
In addition , the analysis of a set of DR- , DQ- , DP+ homozygous deletion mutants ( 721.82 , 721.84 , and 721.101 ) reveals a deletion breakpoint between the DQ alpha 1 / DQ beta 1 loci and the DQ alpha 2 / DQ beta 2 loci . - pubmed
Among the 156 ampicillin - susceptible , beta - lactamase - negative isolates , synergy was detected in 13 with ampicillin - cefotaxime , and antagonism was detected in only 1 ; in contrast , synergy was found in only 2 strains with ampicillin - chloramphenicol , and antagonism was found in 15 . - pubmed
[ Diuretic effects of ethacrynic acid ( E.C.A. ) II . - pubmed
Interaction of the benzodiazepine antagonists , CGS 8216 and Ro 15 - 1788 , with central adenosine A-1 receptors . - pubmed
Although the potent benzodiazepine antagonist CGS 8216 has in vitro activity indicative of an interaction with central adenosine systems , neither it , nor the benzodiazepine antagonist Ro 15 - 1788 showed any great degree of interaction with central adenosine A-1 receptors as measured by radioligand binding . - pubmed
It is concluded that interaction of benzodiazepine antagonists with A-1 receptors is not a property related to their antagonist activity . - pubmed
She was treated with the calcium channel blocking drug nifedipine , with significant relief of symptoms . - pubmed
The results show no significant deterioration in renal function as determined by serum creatinine or creatinine clearance , as a function of years post - uninephrectomy or age at the time of donation . - pubmed
Uterine ERc and PRc , which sedimented at the 8S and 4S regions , respectively , of a 5 - 20 % sucrose gradient , showed similar rise and decline patterns before and after parturition . - pubmed
Ephedrine - saline nasal wash in allergic rhinitis . - pubmed
BACKGROUND : Ephedrine nasal wash ( 0.25 % to 1 % ) was once used in the treatment of sinusitis and other nasal and paranasal symptoms , but there are no reported controlled trials in allergic rhinitis . - pubmed
RESULTS : Use of ephedrine - saline nasal wash resulted in significant improvement in symptom scores and peak nasal inspiratory flow rates as compared with placebo ( p & lt ; or = 0.001 ) . - pubmed
CONCLUSION : A 1 % ephedrine - saline nasal wash is an inexpensive and effective treatment for allergic rhinitis . - pubmed
After oral PSL ( 30 mg ) , which was given at 7:00 ( AM ) on day 2 , lymphocytes and almost all lymphocyte subsets , except for CD16+CD57- cells , were significantly decreased ; these changes recovered between 13 and 26 hours after PSL administration . - pubmed
The circadian variations and PSL - induced alterations of lymphocyte subsets were relatively comparable , but PSL administration cause a decrease in a wider range of lymphocyte subsets including relatively corticosteroid - resistant subsets such as CD45RA+CD4 + cells , CD8 + cell and NK cell subsets. - pubmed
BACKGROUND : Glutaminase , the principal enzyme of glutamine hydrolysis , breaks down glutamine to supply energy and intermediates for cell growth and is present in high concentrations in replicating tissues such as intestinal epithelium and malignant tumors . - pubmed
These reductions in message and protein translated into a 60 - 80 % decrease in functional glutaminase - specific activity . - pubmed
CONCLUSIONS : This IFN - gamma - mediated decrease in glutaminase activity may be one mechanism by which gut glutamine metabolism is diminished as the tumor grows and becomes the principal organ of glutamine use . - pubmed
BACKGROUND / AIMS : Recently we showed that doses of cholecystokinin octapeptide ( CCK-8 ) that produce physiological plasma CCK levels act via stimulation of afferent vagal pathway to mediate pancreatic enzyme secretion . - pubmed
Vagotomy or perivagal application of capsaicin , a sensory neurotoxin , abolished increases in pancreatic secretion but not plasma CCK levels in response to diversion of bile pancreatic secretion or duodenal administration of casein . - pubmed
Simultaneous prodrug cleavage by nasal carboxylesterase was also noted in the case of hexanoate . - pubmed
Reassortants between these strains indicated that the 51Cr release phenotype segregates with the M2 gene segment . - pubmed
Biochemical studies indicated that the ISVPs ' acquisition of the capacity to induce 51Cr release followed the cleavage of the viral M2 gene product mu 1 / mu 1C to fragments delta and phi during virion conversion to ISVP but did not directly correlate with this cleavage . - pubmed
The chimeric subunit r1(25)r8 , containing 25 amino acid residues from subunit 1 , had the same low activity with alkenal substrates as that displayed by subunit 1 . - pubmed
Mutation of Ala-12 into Gly in r1(25)r8 gave rise to the high alkenal activity characteristic of subunit 8 , showing the importance of amino acid residue 12 for the activity . - pubmed
The major symptoms of Parkinson disease ( PD ) are tremors , hypokinesia , rigidity , and abnormal posture , caused by the degeneration of dopamine ( DA ) neurons in the substantia nigra ( SN ) and deficiency of DA in the neostriatal DA terminals . - pubmed
Norepinephrine ( NE ) and serotonin ( 5 - HT ) levels in the neostriatum and tyrosine hydroxylase and melanin pigments in the substantia nigra are also decreased , and brain cholinergic activity is increased . - pubmed
Increased methylation can deplete DA , NE , and 5 - HT ; increase acetylcholine ; and cause hypokinesia and tremors . - pubmed
A mutant JEM1p carrying a mutation in the highly conserved His - Pro - Asp sequence in the J - domain could not complement either temperature - sensitive growth of the Deltajem1 Deltascj1 double mutant or defects in karyogamy of the Deltajem1 mutant . - pubmed
This paper describes the process of dimer assembly of mitochondrial very - long - chain acyl - CoA dehydrogenase ( VLCAD ) subunit . - pubmed
Decreased immunostaining for TGF - beta RII in adenomas persisted for at least 8 mo after exposure to urethane , whereas immunostaining for TGF - beta1 , - beta2 , and - beta3 and TGF - beta RI persisted at levels comparable to those in normal bronchioles . - pubmed
Inhibition of corticotropin releasing factor ( CRF)-induced adrenocorticotropin ( ACTH ) secretion in pituitary corticotropic cells by substance P. - pubmed
Substance P ( SP ) is one of the three distinct peptides of tachykinin system which possess a common spectrum of biological activities including a modulation of stress . - pubmed
In the same way SP inhibited the CRF - induced accumulation of cyclic adenosine monophosphate ( cAMP ) , indicating that SP influenced the signal transduction pathway of CRF receptor activation . - pubmed
These experiments , as well as others assaying the strength of factor association in high - salt sucrose gradients , lead us to conclude that Upf1p is tightly bound to the smallest polyribosomes , but not to the 40S or 60S ribosomal subunits . - pubmed
Using sucrose gradient fractionation and Northern blotting , we can detect the polysomal association of a PGK1 mRNA decay intermediate and conclude that mRNA decay commences while an mRNA is still being translated . - pubmed
The effects of interfering with GTP - binding proteins on the activation mechanism of calcium release - activated calcium current . - pubmed
It has been suggested that , in some cell types , the link is accomplished by either a small or a heterotrimeric GTP - binding protein , which is inhibited by guanosine 5 '- O -( 3 - thiotriphosphate ) ( GTP [ gamma - S ] ) and , in some cases , pertussis toxin . - pubmed
Putting together a physician practice group and then creating the management and administrative structure for that practice to financially succeed is one of the greatest challenges health systems face today . - pubmed
Erythromycin resistance mutations in ribosomal proteins L22 and L4 perturb the higher order structure of 23 S ribosomal RNA . - pubmed
Thus , while L22 and L4 bind primarily to domain I of 23 S rRNA , erythromycin resistance mutations in these ribosomal proteins perturb the conformation of residues in domains II , III and V and affect the action of antibiotics known to interact with nucleotide residues in the peptidyl transferase center of domain V. These results support the hypothesis that ribosomal proteins interact with rRNA at multiple sites to establish its functionally active three - dimensional structure , and suggest that these antibiotic resistance mutations act by perturbing the conformation of rRNA . - pubmed
Alkaline proteinase and sodium hydroxide were used before phenol / chloroform extraction to achieve high quality DNA from very old samples . - pubmed
Immunohistochemical tests using anti - macrophage complement receptor 3 ( OX42 ) or anti - monocyte - macrophage ( ED1 ) and DiI - ac - low - density lipoprotein ( LDL ) uptake were used to identify microglia . - pubmed
Interaction of the EEA1 FYVE finger with phosphatidylinositol 3 - phosphate and early endosomes . - pubmed
Here we have investigated the structural requirements for the interaction of the FYVE finger of the early endosome antigen EEA1 with PtdIns ( 3 ) P and early endosomes . - pubmed
These results support recently proposed models for the ligand binding of the FYVE domain and indicate that PtdIns ( 3 ) P binding is crucial for the localization and function of EEA1 . - pubmed
Diacylglycerol mediates the T - cell receptor - driven Ca ( 2 + ) influx in T cells by a novel mechanism independent of protein kinase C activation . - pubmed
In this work , the role of diacylglycerol ( DAG ) as the mediator of T - cell receptor ( TCR)-driven Ca ( 2 + ) influx in T cells was investigated . - pubmed
Such stimulation was blocked by Ni ( 2 + ) , a divalent cation known to block Ca ( 2 + ) channels . - pubmed
Inhibition of protein kinase C ( PKC ) by calphostin C did not inhibit , but slightly enhanced , the DAG - stimulated Ca ( 2 + ) entry . - pubmed
However , inhibition of DAG metabolism by DAG kinase and lipase inhibitors enhanced the DAG - stimulated Ca ( 2 + ) entry . - pubmed
DAG lipase and kinase inhibitors also enhanced the Ca ( 2 + ) entry in T cells stimulated through TCR / CD3 complex with anti - CD3 antibody . - pubmed
Calphostin C did not affect the anti - CD3 - stimulated Ca ( 2 + ) entry . - pubmed
These results showed that TCR - driven Ca ( 2 + ) influx in T cells is mediated by DAG through a novel mechanism(s ) independent of PKC activation . - pubmed
The main quercetin conjugate was identified as rutin ( quercetin 3 - rhamnosylglucoside ) by LC - MS . - pubmed
Mutational analysis of invariant valine B12 in insulin : implications for receptor binding . - pubmed
An invariant residue , valine B12 , is part of the insulin B - chain central alpha - helix ( B9 - B19 ) , and its aliphatic side chain lies at the surface of the hydrophobic core of the insulin monomer in close contact with the neighboring aromatic side chains of phenylalanines ( B24 and B25 ) and tyrosines ( B26 and B16 ) . - pubmed
To investigate in detail the role of the B12 residue in insulin - receptor interactions , we have synthesized nine analogues bearing natural or unnatural amino acid replacements for valine B12 by chemical synthesis of modified insulin B - chains and the subsequent combination of each synthetic B - chain with natural insulin A - chain . - pubmed
Our results indicate that beta - branched aliphatic amino acids are generally tolerated at the B12 position with specific steric preferences and that the receptor binding potencies of these analogues correlate with their abilities to form dimers . - pubmed
Furthermore , the structure - activity relationships of valine B12 are quite similar to those of valine A3 , suggesting that valine residues at both A3 and B12 contribute to the insulin - receptor interactions in a similar manner . - pubmed
Using immunoblotting , a more than threefold increase in immunoreactive ROMK levels was observed in the outer medulla after dDAVP infusion . - pubmed
Restriction of water intake to increase vasopressin levels also significantly increased TAL ROMK immunolabeling and abundance in immunoblots . - pubmed
In addition , dietary Na (+ ) levels were varied to determine whether ROMK abundance was also affected under other conditions known to alter TAL transport . - pubmed
Infusion of the alpha ( 2 )- adrenoceptor agonist clonidine decreased extracellular noradrenaline in the LC as well as in the PFC . - pubmed
Infusion of the alpha ( 2A )- adrenoceptor antagonist BRL44408 , or the alpha ( 1 )- adrenoceptor agonist cirazoline into the LC or PFC caused a similar dose - dependent increase in both structures . - pubmed
Infusion of the 5 - HT ( 1A )- receptor agonist flesinoxan into the LC or the PFC decreased the release of noradrenaline in both structures . - pubmed
Ganglioside GD1a inhibits HGF - induced motility and scattering of cancer cells through suppression of tyrosine phosphorylation of c - Met . - pubmed
The c - Met in the cells was autophosphorylated by stimulation with HGF , but after treating the cells with GD1a , the HGF - induced autophosphorylation of c - Met was suppressed . - pubmed
These results suggest that GD1a acts as a negative regulator of c - Met in cancer cells . - pubmed
Demethylation treatment by 5 - aza-2 '- deoxycytidine restored AR mRNA expression in PLS20 . - pubmed
The measurement of iNOS activity , iNOS protein formation and NO concentration in the ischemic core and penumbra , and the determination of infarct volume were performed at 6 , 12 , 24 and 48 h after reperfusion . - pubmed
The time course of iNOS protein , enzymatic activity and NO concentration in the penumbra but not in the core matched the process of infarct maturation . - pubmed
Treatment with iNOS inhibitor aminoguanidine ( 100 mg.kg(-1 ) , i.p . ) - pubmed
at 6 and 12 h after reperfusion inhibited iNOS activity by 88.0 + /- 10.4 % and reduced NO concentration by 48.5 + /- 8.3 % in the penumbra , and lessened infarct size by 48.8 + /- 7.2 % . - pubmed
The iNOS activity and NO level in the core were not affected by the administration of aminoguanidine . - pubmed
NTP Toxicology and Carcinogenesis Studies of Furfural ( CAS No . - pubmed
In particular , TAP has stereoselectivity for amino acid derivative substrates , hence it is useful for the kinetic resolution of racemic mixtures of industrial chemicals . - pubmed
The structure of TAP in complex with a diethyl phosphono moiety ( DEP ) identified its catalytic triad , Ser10 - Asp154 - His157 , and oxyanion hole , Ser10 - Gly44 - Asn73 . - pubmed
The oxyanion hole of TAP consists of three residues each separated from the other by more than 3.5A , implying that all of them are highly polarized when substrate bound . - pubmed
The catalytic ( His)C(epsilon1)-H ... O = C hydrogen bond usually plays a role in the catalytic mechanisms of most serine hydrolases , however , there were none present in SGNH - hydrolases . - pubmed
Through comparison of TAP and the three SGNH - hydrolase structures currently known , we have identified a unique hydrogen bond network which stabilizes the catalytic center : a newly discovered structural feature of SGNH - hydrolases . - pubmed
[ Inhibition of hepatic glucose-6 - phosphatase by fatty acids and zinc ions ] . - pubmed
Attenuation of renal vasopressin V2 receptor upregulation by bosentan , an ETA / ETB receptor antagonist . - pubmed
The goal of the present studies was to determine if ET-1 is involved in upregulating the expression of AVP V2 mRNA in the IMCD of CM by using a mixed ETA / ETB receptor antagonist bosentan . - pubmed
The effect of bosentan in normalizing the expression of AVP V2 and AQP2 mRNA in the IMCD of CM was confirmed by in situ hybridization studies . - pubmed
Two distinct methods analyzing chromatin structure using centrifugation and antibodies to modified histone H3 : both provide similar chromatin states of the Rit1 / Bcl11b gene . - pubmed
One is ChIP assay examining the degree of enrichment with histone H3 methylated at lysine 9 ( H3 - mLys9 ) in chromatin and the other is H / E ( heterochromatin / euchromatin ) assay that measures a chromatin condensation state by using centrifugation . - pubmed
Mutations were identified in homozygous F3 progeny derived from ENU - treated founder males . - pubmed
Aberrant methylation of a sodium co - transporter , solute carrier family 5 member 8 gene ( SLC5A8 ) , has been detected in a subset of colorectal cancers , suggesting SLC5A8 may also serve as a tumor suppressor . - pubmed
Cells of all three cell lines died by apoptosis after exposure to 1 microg / ml nocodazole , a mitotic blocker that acts by inhibiting the polymerization of tubulin ( & gt;25 % apoptotic cells after 24 h ) . - pubmed
The phosphatidylinositol 3 - kinase ( PI3 K ) signaling pathway was also involved since the PI3 K inhibitor , LY294002 , abolished the TIMP-1 - mediated growth stimulation . - pubmed
Mechanisms of adaptations for oxytocin neurones are reviewed , using challenge with systemic interleukin-1beta , simulating activation of immune signaling by infection , as a stressor of special relevance in pregnancy . - pubmed
This lack of response is reversed by naloxone treatment just before interleukin-1beta administration , indicating endogenous opioid suppression of oxytocin responses in late pregnancy . - pubmed
Finasteride pretreatment , inhibiting progesterone conversion to allopregnanolone , a positive GABA ( A ) receptor allosteric modifier , also restores an oxytocin response to interleukin-1beta . - pubmed
Accordingly , allopregnanolone may both enhance GABA inhibition of oxytocin neurone responses to interleukin-1beta , and induce opioid suppression of noradrenaline release onto oxytocin neurones . - pubmed
BACKGROUND AND PURPOSE : To follow up in vitro evidence that Delta ( 9 )- tetrahydrocannabivarin extracted from cannabis ( eDelta ( 9 )- THCV ) is a CB ( 1 ) receptor antagonist by establishing whether synthetic Delta ( 9 )- tetrahydrocannabivarin ( O-4394 ) and Delta ( 8 )- tetrahydrocannabivarin ( O-4395 ) behave as CB ( 1 ) antagonists in vivo . - pubmed
CONCLUSIONS AND IMPLICATIONS : O-4394 and O-4395 exhibit similar in vitro potencies to eDelta ( 9 )- THCV as CB ( 1 ) receptor ligands and as antagonists of cannabinoid receptor agonists and can antagonize Delta ( 9 )- tetrahydrocannabinol in vivo . - pubmed
In the FTY720 - treated groups , MAdCAM-1 expression on high endothelial venules ( HEVs ) in Peyer 's patches ( PPs ) was upregulated and its expression on endothelial cells of vessels in the lamina propria was downregulated in comparison with the allograft group without FTY720 . - pubmed
Vitamin E supplementation enhances hemoglobin and erythropoietin levels in mildly anemic adults . - pubmed
In this paper , we test this model by creating procyclic and bloodstream form null mutants of TbALG3 , the gene that encodes the alpha - mannosyltransferase that converts Man5GlcNAc2 - PP - Dol to Man6GlcNAc2 - PP - Dol . - pubmed
However , both forms display aberrant N - glycosylation of their major surface glycoproteins , procylcin , and variant surface glycoprotein , respectively . - pubmed
Specifically , procyclin and variant surface glycoprotein N - glycosylation sites that are modified with Man9GlcNAc2 and processed no further than Man5GlcNAc2 in the wild type are glycosylated less efficiently but processed to complex structures in the mutant . - pubmed
Moreover , mutation of the predicted catalytic cysteine led to the same level of attenuation as a null mutant , suggesting that the G6 protein has enzymatic activity that is important in vivo . - pubmed
This treatment was also more effective than that with Se or Zn alone in reversing Cd - induced decrease in serum T4 level and Cd - induced increase in serum TSH level . - pubmed
The median lethal dose ( LD50 ) of raddeanin A lavage was 1.1 g / kg and that of raddeanin A injection was 16.1 mg / kg . - pubmed
With Avicel as carbon source the transcript level of cel5A continuously increased until the 10th hour of incubation and then a sharp decrease was observed , whereas cel5B presented a slow constitutive expression on this substrate . - pubmed
Somatolactin is thought to be involved in a wide range of physiological actions , including reproduction , stress response , the regulation of Ca ( 2 + ) and acid - base balance , growth , metabolism , and immune response . - pubmed
Sequence analysis of purified SL revealed that the prehormone is composed of a signal peptide of 23 amino acids and a mature protein of 207 amino acids , which has a possible N - glycosylation site at position 121 and seven Cys residues . - pubmed
The objective of this in silico study is to elucidate the pharmacokinetic origins of incomplete viral suppression , exemplified for zidovudine ( AZT ) as a representative of the key class of nucleoside reverse transcriptase inhibitors ( NRTIs ) . - pubmed
Inhibition of the cannabinoid type-1 receptor ameliorates the effects of endocannabinoids on food intake and energy metabolism ; lipogenesis is inhibited , lipolysis , fatty acid oxidation and glucose uptake increase . - pubmed
At discharge , only serum sodium , log - transformed BNP , IVC size , and collapsibility were statistically significant predictors of readmission . - pubmed
Moreover , by means of a sensitive electrochemiluminescent immunoassay employing Abeta C - terminal specific antibodies , mature oligodendrocytes are shown to secrete the 40 and 42 amino acid Abeta species ( Abeta40 and Abeta42 ) . - pubmed
The addition of PrP(Sc ) or PrP82 - 146 caused a dose - dependent reduction in the synaptophysin content of PrP wildtype neurones indicative of synapse damage . - pubmed
Pre - treatment of cortical or hippocampal neurones with docosahexaenoic ( DHA ) or eicosapentaenoic acids ( EPA ) protected neurones against the loss of synaptophysin induced by PrP82 - 146 or PrP(Sc ) . - pubmed
This effect of DHA and EPA was selective as they did not alter the loss of synaptophysin induced by a snakevenom neurotoxin . - pubmed
Promoter histone H3 lysine 9 di - methylation is associated with DNA methylation and aberrant expression of p16 in gastric cancer cells . - pubmed
Accordingly , we chose p16 to clarify whether there is a correlation among histone H3 lysine 9 ( H3 - K9 ) di - methylation , H3 - K9 acetylation , DNA methylation and p16 expression in human gastric cancer . - pubmed
We investigated p16 promoter DNA methylation status , p16 mRNA levels , regional and global levels of di - methyl - H3 - K9 and acetyl - H3 - K9 in four groups : i ) 5 - Aza - dC , ii ) TSA , iii ) the combination of 5 - Aza - dC and TSA and iv ) control group with no treatments . - pubmed
Treatment with TSA , increased H3 - K9 acetylation at the hypermethylated promoter , but did not affect H3 - K9 di - methylation or p16 expression . - pubmed
By contrast , treatment with 5 - Aza - dC , reduced H3 - K9 di - methylation , increased H3 - K9 acetylation at the hypermethylated promoter and reactivated the expression of p16 . - pubmed
In addition , 5 - Aza - dC and the combined treatment did not result in global alteration of H3 - K9 di - methylation . - pubmed
In addition to its effect on DNA methylation , 5 - Aza - dC can act at histone modification levels to reactivate p16 expression in region - specific and DNA methylation - dependent manner . - pubmed
Presumptive Estrogen Target Neurons Express mRNAs for both the Neurotrophins and Neurotrophin Receptors : A Basis for Potential Developmental Interactions of Estrogen with the Neurotrophins . - pubmed
Estrogen and the neurotrophins regulate development , survival , and plasticity of the nervous system . - pubmed
These results suggest a potential for interactions between estrogen and the neurotrophins , including possible estrogen - stimulated , neurotrophin - mediated autocrine mechanisms that may regulate neuronal differentiation and survival during development . - pubmed
A self - produced radioimmunoassay and relevant kits were used to detect the protein level of orexin - A in the plasma and the hypothalamus , serum glucose , total anti - oxidation capacity and alanine transaminase , HE staining and immunohistochemistry were used to investigate the pathological variation and protein expression of orexin - A in the liver , while RT - PCR was applied to observe mRNA expression of orexin - A in the hypothalamus and the liver . - pubmed
There was significant and positive linear correlation between the plasma orexin - A and serum glucose and total anti - oxidation capacity ; serum alanine transaminase in each reperfusion group was significantly higher , and liver damage was significantly alleviated at the later phase of the injury . - pubmed
Then , by combining the molecular weight of the so - obtained homopolymers , as measured by MS , with the relative molar proportion of the MMA and MMA units , as given by ( 1 ) H NMR spectrum , one can straightforwardly estimate the molecular weight of the initial copolymer . - pubmed
Hb Perpignan [ beta136(H14)Gly-->Ser ] , a silent variant associated with normal hematology . - pubmed
Inhibition of interleukin-1beta converting enzyme ( ICE or caspase 1 ) by aspartyl acyloxyalkyl ketones and aspartyl amidooxyalkyl ketones . - pubmed
Novel screening cascade identifies MKK4 as key kinase regulating Tau phosphorylation at Ser422 . - pubmed
Phosphorylation of Tau at serine 422 promotes Tau aggregation . - pubmed
An overexpression of netrin2 at these two developmental stages suggests a novel role for this gene in regulating axonal density specific to Pb neurotoxicity . - pubmed
Ceramidases are key enzymes that control this rheostat by hydrolyzing ceramide to produce sphingosine and may also confer resistance to drugs and radiation . - pubmed
In this study , we identify a novel ceramidase inhibitor ( Ceranib-1 ) by screening a small molecule library and describe the synthesis of a more potent analogue ( Ceranib-2 ) . - pubmed
In a cell - based assay , both compounds were found to inhibit cellular ceramidase activity toward an exogenous ceramide analogue , induce the accumulation of multiple ceramide species , decrease levels of sphingosine and S1P , inhibit the proliferation of cells alone and in combination with paclitaxel , and induce cell - cycle arrest and cell death . - pubmed
AFB ( 1 ) activation leads to DNA adduction and mutagenesis , with a specific mutation at codon 249 in TP53 ( p. R249S ) . - pubmed
Conversely , HBV infection did not significantly modify AFB ( 1 )-DNA adduct formation or repair as assessed by immunodot - blot assay , and the induction of p53 in response to AFB ( 1 ) was similar in infected and non - infected HepaRG cells . - pubmed
The iAbeta5p beta - breaker peptide regulates the Abeta(25 - 35 ) interaction with lipid bilayers through a cholesterol - mediated mechanism . - pubmed
In this work , we focused on the effect of the beta - sheet breaker peptide acetyl - LPFFD - amide , iAbeta5p , on the interaction of the Abeta(25 - 35 ) fragment with lipid membranes , studied by Electron Spin Resonance spectroscopy , using spin - labeled membrane components ( either phospholipids or cholesterol ) . - pubmed
The ESR results show that iAbeta5p influences the Abeta(25 - 35 ) interaction with the bilayer through a cholesterol - mediated mechanism : iAbeta5p withholds cholesterol in the inner hydrophobic core of the bilayer , making the interfacial region more fluid and capable to accommodate Abeta ( 25 - 35 ) . - pubmed
Concretely , AuNPs having poly ( 4 - vinylpyridine ) ( P4VP ) on the surface were first fabricated through surface - initiated atom transfer radical polymerization ( SI - ATRP ) . - pubmed
The bimetallic nanostructures of AuNP@P4VP nanocomposites containing another type of metal NP at the P4VP periphery exhibit a controlled sensing property in terms of the change in the refractive index of surrounding media and a typical electrocatalytic activity for methanol oxidation reaction . - pubmed
Fasting blood glucose , oral glucose tolerance , serum lipid profile , tissues glycogen content , glucose 6 - phosphatase and hexokinase enzymes level in liver , in vitro and in vivo insulin estimation were measured on last day of treatment . - pubmed
High glucose predisposes gene expression and ERK phosphorylation to apoptosis and impaired glucose - stimulated insulin secretion via the cytoskeleton . - pubmed
Chronic HG exposure which reduced glucose - stimulated insulin secretion ( GSIS ) increased transcript levels of 185 genes that clustered primarily in 5 processes namely cellular growth and proliferation ; cell death ; cellular assembly and organization ; cell morphology ; and cell - to - cell signaling and interaction . - pubmed
These studies revealed a depolymerized actin cytoskeleton that lacked actin stress fibers anchored at vinculin - containing focal adhesion sites as well as loss of E - cadherin - mediated cell - cell adherence after exposure to chronic HG , and were concomitant with constitutive ERK1 / 2 phosphorylation that was refractory to serum and glucose deprivation . - pubmed
Although inhibition of ERK phosphorylation by PD98059 promoted actin polymerization , it increased apoptosis and GSIS impairment . - pubmed
Co - over - expression of MNSFbeta and Bcl - G reduced S - nitrosoglutathione - induced ERK1 / 2 phosphorylation . - pubmed
Glycoprotein misfolding in the endoplasmic reticulum : identification of released oligosaccharides reveals a second ER - associated degradation pathway for Golgi - retrieved proteins . - pubmed
In the present work , analysis of the released , free oligosaccharides ( FOS ) derived from all glycoproteins undergoing ERAD , has allowed a global estimation of the mechanisms of this pathway rather than following model proteins through degradative routes . - pubmed
The Glc3Man7GlcNAc2 FOS species has been shown to be produced in the ER lumen by a mechanism involving a peptide : N - glycanase - like activity , and its production was sensitive to disruption of Golgi - ER trafficking . - pubmed
The detection of this oligosaccharide was unaffected by the overexpression of EDEM1 or cytosolic mannosidase , both of which increased the production of previously characterised cytosolically localised FOS . - pubmed
Ecto-5 '- nucleotidase ( CD73 ) is expressed on intestinal epithelial cells , and it is hypothesized to protect against toxin - induced epithelial damage through the cleavage of 5 '- AMP to adenosine ( Ado ) and subsequent activation of adenosine receptors ( AdoRs ) . - pubmed
Transepithelial electrical resistance ( TEER ) , paracellular fluorescein isothiocyanate ( FITC)-dextran flux , and tight junction protein ( ZO-1 ) integrity were monitored . - pubmed
Concurrent treatment with 5 '- AMP attenuated these responses to Cdf toxin ; an effect that was blocked with ZM241385 ( AdoRA2 antagonist ) . - pubmed
APCP , a CD73 inhibitor , also suppressed the protective effects of 5 '- AMP on paracellular flux . - pubmed
5 '- AMP reduced toxin - induced disruption of ZO-1 , an effect that was abolished by APCP and ZM241385 . - pubmed
Inhibition of CD73 with APCP during Cdf toxin exposure led to increased intestinal barrier permeability and epithelial damage in vivo . - pubmed
5 '- AMP treatment attenuated the damaging effects of Cdf toxin in vitro , and inhibitors of CD73 ( APCP ) and AdoRs ( ZM241385 ) revealed that the cleavage of 5 '- AMP to Ado was necessary for the protective effects . - pubmed
We showed previously that three cytoplasmic Ser / Thr kinases , LKB1 , SAD - A , and SAD - B , control early axon - dendrite polarization in forebrain neurons . - pubmed
The convulsant effects of alpha - thujone are attributed to inhibitory actions on the GABAA receptor . - pubmed
However , it should not be discarded that acute stress associated with novelty may have induced the recruitment of a subpopulation of GABAA receptors more sensitive to alpha - thujone than to the constitutive receptors , or that this monoterpene could have inhibited any protein or enzyme trafficking that modulated the phosphorylation of the receptor involved in the turnover of GABAA receptor . - pubmed
Filgrastim is a rhG - CSF analogue and is marketed under various brand names , including Neupogen ( ) ( Amgen ) , Imumax ( ) ( Abbott Laboratories ) , Neukine ( ) ( Intas Biopharmaceuticals ) and others . - pubmed
The enzyme 5alpha - reductase catalyzes the transformation of progesterone , testosterone , and deoxycorticosterone into 5alpha - reduced metabolites , which are recognized as neurosteroids in the brain with variable potential neuroactivity . - pubmed
Interestingly , immunolocalization studies with antibodies targeting peroxiredoxin indicate differential localization of the protein in the cell during metronidazole stress . - pubmed
In normal conditions , all the Entamoeba isolates exhibit presence of peroxiredoxin in the nucleus as well as in the membrane ; however with metronidazole stress the protein localized mostly to the membrane . - pubmed
Colocalization pattern of peroxiredoxin with CaBp1 , a cytosolic protein , revealed that the membrane and nuclear localization was specific to peroxiredoxin during metronidazole stress . - pubmed
Evidence for the participation of Ca ( 2 +)- activated chloride channels in formalin - induced acute and chronic nociception . - pubmed
In this study we determined the role of Ca ( 2 +)- activated chloride channels ( CaCC ) in acute and chronic nociceptive responses elicited by 1 % formalin . - pubmed
Local peripheral and intrathecal pre - treatment ( -10 min ) with the non - selective and selective CaCC blockers niflumic acid and CaCCinh - A01 , respectively , prevented formalin - induced flinching behavior mainly during phase 2 of the formalin test . - pubmed
Moreover , local peripheral and intrathecal post - treatment ( on day 6 ) with both CaCC blockers decreased the established formalin - induced secondary mechanical allodynia and hyperalgesia behavior in both paws . - pubmed
Formalin injection increased anoctamin-1 , but not bestrophin-1 protein levels at 6 days . - pubmed
Intrathecal injection of the CaCC inhibitor CaCCinh - A01 prevented formalin - induced anoctamin-1 increase . - pubmed
Data suggest that peripheral and spinal CaCC , and particularly anoctamin-1 , participates in the acute nociception induced by formalin as well as in the development and maintenance of secondary mechanical allodynia and hyperalgesia . - pubmed
Thus , CaCC activity contributes to neuronal excitability in the process of nociception induced by formalin . - pubmed
Decreases in the levels of C - reactive protein , fibrinogen , and D - dimer were not associated with decreases in the levels of triacylglycerol and low - density lipoprotein cholesterol . - pubmed
The first region accounts for the differences between J-1 and PL-1 gp16 and showed sequence similarity to carbohydrate - binding modules ( CBMs ) . - pubmed
Saponarin ( SA ) , a natural flavonoid , is known for its antioxidant and hepatoprotective activities . - pubmed
In vivo treatments with the pharmacological AnxA1 mimetic peptide ( Ac2 - 26 ) or the formyl peptide receptor ( FPR ) antagonist ( Boc-2 ) were used to elucidate the pathway of AnxA1 action , and with the cytosolic glucocorticoid antagonist receptor RU 38486 . - pubmed
AnxA1 and FPR2 were constitutively expressed in bone marrow granulocytes , and their expressions were reduced by treatment with RU38486 . - pubmed
When present , the severity of periodontitis and its relationship with gender , smoking , age , corticosteroids dose and AR activity were assessed . - pubmed
Further study indicated that berberine inhibited MRSA amyloid fibrils formation , which consist of phenol - soluble modulins ( PSMs ) . - pubmed
Collectively , berberine can inhibit MRSA biofilm formation via affecting PSMs ' aggregation into amyloid fibrils , and thereby enhance bactericidal activity of antibiotics . - pubmed
Upon tamoxifen administration at postnatal day 21 , the floxed mHtt - exon1 was removed and mHtt expression was terminated ( Q97(CRE ) ) . - pubmed
The present study was aimed to investigate the role of curcumin in myocardial infarction ( MI ) and its potential mechanism involving transcription factor specific protein 1 ( SP1 ) . - pubmed
In hypoxia - induced MCM , curcumin led to the decrease of cell apoptosis . - pubmed
Transfected MCM with miR-7a / b inhibitor , curcumin induced the decrease of cell apoptosis and SP1 expression was reversed . - pubmed
Transfected with pcDNA - SP1 , the decrease of cardiac myocytes apoptosis after the treatment of curcumin was also reversed . - pubmed
CONCLUSION : Curcumin pre - treatment protected against hypoxia - induced cardiac myocytes apoptosis through the up - regulation of miR-7a / b and the down - regulation of SP1 expression . - pubmed
To identify proteins essential for the IBB477 specific adhesion property , nine deletion mutants in chromosomal genes have been constructed and analysed using adhesion tests on bare polystyrene as well as mucin- , fibronectin- or collagen IV - coated polystyrene plates in comparison to the wild - type strain . - pubmed
These experiments demonstrated that gene AJ89_07570 encoding a protein containing DUF285 , MucBP and four Big_3 domains is involved in adhesion to bare and mucin - coated polystyrene . - pubmed
To avoid aeration and enhance oxygen - utilization efficiency , columnar activated carbon with half of its surface coated by polytetrafluoroethylene ( PTFE - coated CAC ) was fabricated as biocathode material , and its performance was investigated using a tide - type biocathode MES ( TBMES ) . - pubmed
The TBMES with PTFE - coated biocathode achieved a maximum power density of 8.2 + - 0.8 W m-3 , which was 39 % higher than that of the untreated control ( CAC biocathode ) . - pubmed
The transaminase ( TAm ) catalyzed kinetic resolution of racemic amino alcohols provides a straightforward approach to access these important compounds . - pubmed
Moreover , this method can be used to detect transaminase activity and enantioselectivity toward amino alcohols in a high - throughput format . - pubmed
The endocytosis of N - cadherin , a calcium - dependent adhesion molecule , has been implicated in the regulation of neurite outgrowth , but the mechanism remains unclear . - pubmed
Two tyrosine - based motifs in the cytoplasmic domain of N - cadherin recognized by the mu2 subunit of the AP-2 adaptor complex are responsible for CME of N - cadherin . - pubmed
Moreover , beta - catenin , a core component of the N - cadherin adhesion complex , inhibits N - cadherin endocytosis by masking the 2 tyrosine - based motifs . - pubmed
RESULTS : The chemical combination of 5 - hydroxymethyl-2 - furaldehyde ( 5 - HMF ) , parishin B ( PB ) and parishin C ( PC ) was discovered and confirmed as QCMs of GET by the OPLS - DA method . - pubmed
No infant in the MSC group was discharged with home supplemental oxygen compared with 22 % in the comparison group . - pubmed
The NP samples were prepared by the thermal decomposition of an organic iron precursor subjected to varying reaction conditions , yielding variations in the NP size , shape and relative crystallinity . - pubmed
These reduce low - density lipoprotein cholesterol by over 50 % across a range of populations and were generally found to have a safety profile comparable with placebo . - pubmed
The ODYSSEY OUTCOMES trial comparing alirocumab to placebo is expected to report in 2018 and provide cardiovascular outcome data in a post acute coronary syndrome population . - pubmed
The phase II ORION-1 study using inclisiran , a small interference RNA to PCSK9 , suggested that two doses of inclisiran produced time averaged reductions in LDL cholesterol of 50 % over 9 months . - pubmed
The analysis of the same polymorphisms showed association at the P value & lt;0.05 with nutrients ( fat , SFA , and polyunsaturated fatty acid - PUFA and saccharose ) and elevated LDL level . - pubmed
The majority of patients in both & gt;=50 and & lt;50 age groups received a non - nucleoside reverse transcriptase inhibitor - based regimen ( 89 % vs. 87 % ) , did not have AIDS at baseline ( 63 % vs. 62 % ) , and were male ( 59 % vs. 58 % ) . - pubmed
AIM : This study aims to explore the accuracy , specificity and laws of axillary lymph node metastasis predicted by sentinel lymph node biopsy ( SLNB ) by comparing axillary lymph node status via SLNB and axillary lymph node dissection ( ALND ) with nanocarbon as the tracer . - pubmed
Hypermethylated cytosines were evenly distributed throughout the genome with a slight preference for AP-1 ( JUN and FOS ) binding sites . - pubmed
Hypermethylated cytosines tended to be enriched within the binding sites of transcription factors that showed increased gene expression after VHL inactivation . - pubmed
The present study aims to investigate whether ONO-5046 , a novel neutrophil elastase inhibitor , could affect NETs formation and promote inflammation resolution . - pubmed
ROS was detected by DCFH - DA fluorescent staining and NETs formation was demonstrated via immunofluorescence staining for neutrophil elastase and citrullinated histone H3 ( H3Cit ) . - pubmed
ONO-5046 reduced LPS - induced NETs formation in a dose - dependent manner , as evidenced by immunofluorescence staining for neutrophil elastase and H3Cit whereby the fluorescence intensity decreased and neutrophil ROS generation was attenuated . - pubmed
CONCLUSIONS : The neutrophil elastase inhibitor ONO-5046 suppresses ROS - associated NETs formation , which may lead to inflammation resolution . - pubmed
In this study , we constructed a pharmacophore model based on a series of flavin adenine dinucleotide ( FAD)-competing inhibitors bearing triazole - dithiocarbamate scaffold combining docking , structure - activity relationship ( SAR ) study , and molecular dynamic ( MD ) simulation . - pubmed
On the basis of the two pharmacophore models , multi - step virtual screenings ( VSs ) were performed against substrate - binding pocket and FAD - binding pocket , respectively , combining pharmacophore - based and structure - based strategy to exploit novel LSD1 inhibitors . - pubmed
Considering the binding mode and SAR of the series of compounds , we could roughly deem that these compounds containing 3 - methylxanthine scaffold act through occupying substrate - binding pocket competitively . - pubmed
Total plasma triglyceride and VLDL - triglyceride concentrations decreased after calorie restriction and exercise ( all p & lt;= 0.025 ) ; the pattern of change was linear with an increasing energy deficit ( all p & lt ; 0.03 ) , with no evidence of plateauing . - pubmed
Our data indicate that an acute negative energy balance induced by calorie restriction and aerobic exercise reduces triglyceride concentrations in a dose - dependent manner , by decreasing circulating large and medium VLDL particles . - pubmed
Amitriptyline attenuates bleomycin - induced pulmonary fibrosis : modulation of the expression of NF - kappabeta , iNOS , and Nrf2 . - pubmed
Lung injury was evaluated through the histological examination and immunohistochemical detection of alpha - smooth muscle actin ( alpha - SMA ) in lung tissue , in addition to the biochemical assessment of pulmonary contents of hydroxyproline and transforming growth factor beta-1 ( TGF - beta1 ) . - pubmed
In addition , the following parameters were investigated for studying the possible mechanisms of amitriptyline antifibrotic effect : inducible nitric oxide synthase ( iNOS ) , nuclear factor - kappabeta ( NF - kappabeta ) , tumor necrosis factor - alpha ( TNF - alpha ) , serpine-1 , p53 , nuclear factor erythroid 2 - related factor 2 ( Nrf2 ) , lipid peroxides , and reduced glutathione ( GSH ) . - pubmed
Amitriptyline successfully suppressed the expression of NF - kappabeta , Nrf2 , iNOS , and p53 in lung tissues besides the inhibition of other oxidative stress and inflammatory mediators . - pubmed
lusitaniae strain for the expression of full - length FKS1 alleles harbouring known mutations contributing to reduced susceptibility or resistance to caspofungin , thus opening the way for the screening of other FKS1 / FKS2 mutations potentially involved in RES . - pubmed
Relative to Delta9 - tetrahydrocannabinol ( THC ) , the synthetic cannabinoid CP 55,940 ( CP ) is significantly more potent and efficacious at cannabinoid receptors , the primary targets for endogenous cannabinoids ( eCBs ) . - pubmed
A potent and selective irreversible CDK7 inhibitor THZ2 was able to induce cell growth inhibition , cell cycle arrest at G2 / M phase and apoptosis with the increasing intracellular reactive oxidative species ( ROS ) levels in gastric cancer cells . - pubmed
Despite surgery and IV vancomycin with MIC of 1 , suggesting extreme susceptibility , the patient 's blood cultures remained persistently bacteremic at day 5 of treatment . - pubmed
Our results demonstrate that overexpression of phytoglobins allows plants to more efficiently use atmospheric NO as N source . - pubmed
Application of CIP after p25 insult reverses the pathological changes and behavioral abnormalities . - pubmed
ROS -mediated p53 activation by juglone enhances apoptosis and autophagy in vivo and in vitro . - pubmed
Further experiments proved that H2O2 was a major factor since the H2O2 scavenger catalase ( CAT ) reduced both autophagy and cell death to a greater extent than the O2 - scavenger SOD . - pubmed
The released ions could not only kill microbes by destroying their membrane and inducing the outflow of nutrient substance , but also activate copper / zinc superoxide dismutase ( Cu / Zn - SOD ) to eliminate oxygen free radicals and rescue the cells from oxidative stress injury . - pubmed
By expressing channelrhodopsin in hESCs - derived glutamate neurons and co - culturing them with GABA neurons , we found that blue light stimulation increased the frequency of miniature excitatory postsynaptic currents ( mEPSCs ) and decreased the ratio of paired pulse facilitation ( PPF ) in non - ChR2 - expressing GABA neurons , indicating a facilitating action at the presynaptic terminals . - pubmed
Rosmarinic acid reverses non - small cell lung cancer cisplatin resistance by activating the MAPK signaling pathway . - pubmed
Our data demonstrated that RA significantly inhibited NSCLC cell proliferation and cell colony formation in a dose - dependent manner , induced G1 phase cell cycle arrest and apoptosis , and increased the sensitivity of cell lines resistant to DDP . - pubmed
Mechanistically , RA inhibited NSCLC cell growth , arrested cell cycle , and induced apoptosis by activating MAPK and inhibiting the expression of P - gp and MDR1 , which correspondingly enhanced p21 and p53 expression . - pubmed
We demonstrate that RA is a potentially effective MDR reversal agent for NSCLC , based on downregulation of MDR1 mRNA expression and P - gp . - pubmed
Bovine serum albumin determination based on methylene blue detection by photothermal lens spectroscopy . - pubmed
We report on a new sensitive method , for bovine serum albumin quantification , which is based on the use of methylene blue as a labelling agent combined with photothermal lens detection . - pubmed
In the presence of sodium dodecyl sulfate , methylene blue forms a dimer which can react with bovine serum albumin producing dedimerization . - pubmed
We found that , the photothermal signal decreases proportional to the concentration of bovine serum albumin added to the system composed by dodecyl sulfate and methylene blue . - pubmed
CONCLUSIONS : We show that staining for AFB on BAL material in lung transplant patients had limited benefit when concurrent microbiology was performed . - pubmed
Bone morphogenetic proteins ( BMPs ) belonging to the transforming growth factor - beta ( TGF - beta ) family control the dorsal - ventral ( DV ) patterning of embryos , whereas other morphogens such as fibroblast growth factor ( FGF ) , Wnt family members , and retinoic acid ( RA ) regulate the formation of the anterior - posterior ( AP ) axis . - pubmed
Trace elements , such as copper ( Cu ) , have been reported to be capable of inhibiting prolyl hydroxylases leading to the accumulation and activation of hypoxia - inducible factor-1alpha ( HIF-1alpha ) , a major transcription factor regulating the expression of VEGF . - pubmed
pastoris cell growth and may regulate the alcohol oxidase1 ( AOX1 ) promoter via regulatory factors activated by methanol - mediated stimulation . - pubmed
Thirdly , the unfolded protein response ( UPR ) and ER - associated degradation ( ERAD ) pathways were not significantly upregulated during the methanol induction period . - pubmed
Finally , the upregulation of the autophagy pathway during the methanol induction phase may be related to the degradation of damaged peroxisomes but not to the production of phytase . - pubmed
After euthanasia performed one month later , histological analysis was made using hematoxylin - eosin and anti - proColXIalpha1 . - pubmed
Modulation of EZH2 , Snail , eIF4E , IMPDH , mTOR , and cyclin D1 were observed in western blots and enzymatic activity assays in response to ribavirin treatment . - pubmed
Our work suggests that NPC responds to ribavirin - mediated EZH2 , Snail , eIF4E , IMPDH , and mTOR changes and positions ribavirin for clinical evaluation as a potential addition to our NPC treatment armamentarium . - pubmed
We then discuss the knotted states that arise in the intrinsically disordered polyglutamine and alpha - synuclein . - pubmed
What 's more , having experienced 8 weeks of FHJ intervention , KEGG pathways of two - component system , ATP - binding cassette ( ABC ) transporters , and biosynthesis of amino acids made the most differentially expressed genes more abundant , the unigene numbers are 16781,480 , and 1,221 , respectively . - pubmed
Here , this study reports a kind of conductive film ( PAM - dc - fGO ) fabricated by cross - linking intrinsic self - repair polyazomethine ( PAM ) and ethylenediamine - functionalized graphene oxide ( fGO ) through dynamic covalent bonds ( imine bonds , -CH N - ) . - pubmed
Renal oxidative stress was also evaluated by measuring superoxide dismutase and malondialdehyde in renal tissues . - pubmed
DNA was removed from the preparation by two cycles of pancreatic DNase digestion and phenol extraction . - pubmed
These additional sequences were contained in AAV RNA molecules present in the pellet fraction , whereas the 20S AAV mRNA accumulated in the supernatant . - pubmed
Increased oncogenic potential of ErbB is associated with the loss of a COOH - terminal domain serine phosphorylation site . - pubmed
Here we show that the mutation of erbB at this negative regulatory serine phosphorylation site causes fibroblast transformation in vitro and is associated with an increased oncogenic potential in vivo . - pubmed
This requirement questions the significance of the homology between La / SS - B and a retroviral gag polypeptide in the generation of the B cell response to La / SS - B and is in accord with the general concept that B cells recognize conformational epitopes on antigens rather than small linear peptide sequences . - pubmed
Spare receptors were detected for oxotremorine - M , methacholine and carbachol , but not the rest of the agonists , by comparing the receptor - occupancy curves with the concentration - response curves . - pubmed
A very sensitive and specific method for the simultaneous assay of the activities of two key regulatory enzymes in cholesterol metabolism , 3 - hydroxy-3 - methylglutaryl - coenzyme A ( HMG - CoA ) reductase ( EC 1.1.1.34 ) , and cholesterol 7 alpha - hydroxylase ( EC 1.14.13.7 ) , is described . - pubmed
Response rates during DD indicated a dissociation of rate depressant effects and discriminative performance following ethanol . - pubmed
The technique of competitive chromogenic substrate hydrolysis is used to examine the inhibitory effects of sucrose and glycerol on the inactivation of thrombin by antithrombin III . - pubmed
The evidence suggests that a component from each of the mitochondrial NADH - coenzyme Q , succinate - coenzyme Q , and coenzyme QH2 - cytochrome c reductases ( complexes I , II , and III ) , most likely a nonheme iron - sulfur protein of each complex , is involved in free radical formation . - pubmed
Continuous VP infusion reduced significantly 24 - h amounts of WI and UO , and increased Na+ excretion . - pubmed
Substance P and 5 - HT do not seem to share a common release mechanism . - pubmed
Changes in antibody production were not related to plasma levels of cortisol , corticosteroid - binding globulin activity , or testosterone . - pubmed
Ca2 + -antagonistic properties of phospholipase A2 inhibitors , mepacrine and chloroquine . - pubmed
Both agents decreased slow calcium channel current Isi and fast sodium channel current If . - pubmed
Role of plasma adenosine in the antiplatelet action of HL 725 , a potent inhibitor of cAMP phosphodiesterase : species differences . - pubmed
The inhibitory action of HL 725 can be reversed by washing and resuspension of the platelets , suggesting that HL 725 does not bind tightly to cAMP PDE . - pubmed
Similar blockade of the inhibitory actions of several other inhibitors of cAMP PDE such as RA 233 , RX - RA 69 ( analogs of dipyridamole ) and oxagrelate is seen by adenosine deaminase pretreatment . - pubmed
However , if the whole blood is pretreated with adenosine deaminase , no inhibitory effect of dipyridamole plus HL 725 is seen on platelet aggregation . - pubmed
Intrastriatal kainic acid injections resulted in a total disappearance of the stimulatory effects of Glu on evoked - release of [ 3H ] DA as well as in a parallel reduction in the maximal number ( Bmax ) of a D - aspartate - insensitive L -[ 3H ] Glu binding site in striatal particulate fractions . - pubmed
There was no significant level of 3H - thymidine uptake by cultures of SLE or normal mononuclear cells in response to either SLE or normal ACS . - pubmed
[ Effects of dithiocarbamate : " in vitro " and " in vivo " changes in gamma - glutamyltransferase induced by sodium diethyldithiocarbamate ] . - pubmed
Experiments of NaDDTC effect on kidney and 10,000 X g liver supernatant fraction have shown no modification of normal GGT activity , also after dialysis . - pubmed
It is suggested that liver MFO produces SH groups derived from NaDDTC metabolism able to interact with GGT and consequential inactivation of the enzyme . - pubmed
beta - Adrenergic blockade with propranolol displaced the glucose production ( MANOVA , P = 0.0220 ) and plasma glucose ( MANOVA , P = 0.0057 ) somatostatin response curves to higher levels , whereas alpha - adrenergic blockade with phentolamine combined with beta - adrenergic blockade displaced the glucose production ( MANOVA , P = 0.0281 ) and plasma glucose ( MANOVA , P = 0.0134 ) somatostatin response curves to lower levels . - pubmed
Nucleoids released from a Ncd clone give two peaks in a sucrose gradient with a characteristic S value for each nucleoid . - pubmed
Mechanism of synthesis of adenosine(5')tetraphospho(5')adenosine ( AppppA ) by aminoacyl - tRNA synthetases . - pubmed
An early and transient postoperative rise in calcitonin ( CT ) corresponding to a decline in calcium levels was demonstrated . - pubmed
The polysaccharide - iron - regulated cell surface protein conjugate ( PS - IRCSP ) was non - toxic and non - pyrogenic . - pubmed
Recombinant alpha 1 - antitrypsin with a P1 arginine residue ( Arg - alpha 1 - antitrypsin ) is a rapid inhibitor of both thrombin and activated protein C ( APC ) . - pubmed
For example , replacement of the P2 proline of Arg - alpha 1 - antitrypsin by glycine decreased the association rate constant ( kass ) with thrombin by 37 - fold while the kass value with APC was reduced by only 16 - fold . - pubmed
The decrease in the kass for thrombin caused by the mutation of the P2 proline to alanine or glycine was 3 - fold greater when threonine was present in the P'1 position instead of the normal serine . - pubmed
Substitution of proline for the P2 glycine of this chimeric serpin increased the kass values with thrombin and APC by 7- and 90 - fold , respectively . - pubmed
The effect of the P2 substitution was again found to depend on the sequence surrounding the residue ; the change in the kass for APC caused by the P2 Pro-->Gly replacement was 6 - fold larger in the chimeric serpin . - pubmed
Evaluation of the kass values of the chimeric serpin with a P2 proline in light of the likely rates of inhibition of thrombin and APC during antithrombotic therapy with heparin suggested that this serpin may have kinetic parameters suitable for an antithrombotic agent . - pubmed
In individual fibers , the activities of malate dehydrogenase ( MDH , citric acid cycle ) , lactate dehydrogenase ( LDH , glycolysis ) and adenylokinase ( high - energy phosphate ) were quantified . - pubmed
Evidence that the inhibitory effects of iodide on thyroid cell proliferation are due to arrest of the cell cycle at G0G1 and G2 M phases . - pubmed
The addition of methimazole at two concentrations ( 0.2 and 2 mM ) to cells exposed to 100 mM NaI prevented the accumulation of cells in G2 M , but did not abolish the accumulation of cells in G0G1 or the reduction in cell number . - pubmed
It appears that organified iodine may mediate the cell cycle arrest in the G2 M phase , whereas inorganic iodide may be responsible for the inhibitory effects at G0G1 . - pubmed
Treatment of membranes with PrBCM results in a selective inactivation of M3 muscarinic receptors , while treatment with EEDQ results in a relative sparing of M1 muscarinic receptors . - pubmed
We have previously shown that dopamine , working through D1 - like dopamine receptors , alters the PLC response to carbachol by preventing this shift in the apparent EC50 for GTP gamma S16 . - pubmed
When we reproduced these experiments in EEDQ- and PrBCM - treated membranes , only in EEDQ - treated membranes was dopamine able to inhibit the PLC response to carbachol . - pubmed
The eventual targets of retinoids are retinoic acid receptors ( RARs ) . - pubmed
Therefore , developmental expression patterns of RARs are important in elucidating the mechanisms of normal functions of retinoids and retinoid - induced teratogenesis . - pubmed
Overlapping expression and redundancy of various RARs may be one of the safeguard mechanisms in retinoid homeostasis . - pubmed
Point mutations of Thr-10 to Arg and Tyr-13 to Ile greatly lowered MCP-1 activity . - pubmed
Insertion of a Pro between these two residues , or their substitution by the sequence Gly - Pro - His , caused nearly complete loss of MCP-1 activity . - pubmed
This sexual dimorphism is apparently also due , in part , to a suppressive effect of circulating androgens on [ leptin ] . - pubmed
Phospholipids inhibit proteolysis of protein kinase C alpha by mM calcium - requiring calpain . - pubmed
The alpha isoform of protein kinase C ( PKC alpha ) is rapidly hydrolyzed by mM Ca ( 2 +)-requiring calpain ( calcium - activated neutral proteinase ) under cell - free conditions ( Shea et al , 1994 , FEBS Lett . - pubmed
With the exception of phosphatidic acid , these phospholipids did not directly inhibit calpain activity as evidenced by degradation of [ 14C ] azocasein , suggesting that the nature of inhibition of calpain - mediated PKC alpha degradation is due to an effect of phospholipids on PKC alpha conformation . - pubmed
In addition , the unique inhibition of calpain activity by phosphatidic acid suggests the existence of a specific mechanism by which this phospholipid regulates PKC alpha activity . - pubmed
The inhibitory effects on sexual behavior and ambulatory activity of the mixed GABAA / GABAB agonist progabide are differentially blocked by GABA receptor antagonists . - pubmed
It was concluded that the GABAA but not the GABAB receptor is important for the inhibitory effects of progabide on that behavior . - pubmed
This suggests that the motor effects of progabide are mediated by either a non - GABAergic receptor or by a subtype of the GABAA or the GABAB receptor that is not sensitive to the antagonists . - pubmed
Their abnormal hemoglobin was about 52 % of the total hemoglobin , and was shown to be stable by the isopropanol test . - pubmed
Whole blood P50 of the proband was 19.3 Torr , Bohr effect was decreased ( -0.25 ) , as well as the molar ratio between the 2,3 - diphosphoglycerate level and total hemoglobin of erythrocytes ( 0.68 ) . - pubmed
The purified abnormal hemoglobin was characterized by an altered oxygen affinity , low n - factor , chloride , and 2,3 - diphosphoglycerate effects . - pubmed
The myelin - associated / oligodendrocyte basic proteins ( MOBPs ) are recently discovered constituents of myelin and are small , cytoplasmic , and highly basic proteins exclusively expressed postnatally by oligodendrocytes . - pubmed
Due to a clustering of positively charged amino acids observed in the most abundant MOBP isoform similar to myelin basic protein ( MBP ) and P0 , it was speculated that MOBP could function in myelin sheath compaction . - pubmed
This ketol isomerase converts the aldose L - fucose into the corresponding ketose L - fuculose using Mn2 + as a cofactor . - pubmed
The protein environment suggests strongly that the reaction belongs to the ene - diol type . - pubmed
Pretreatment with Nomega - nitro - L - arginine methyl ester blocked IGF - I - induced increases in PTA in single myocytes . - pubmed
Consistent with its inotropic actions on muscles and myocytes , IGF - I induced a dose - dependent increase in Ca2 + transients in normal but not diabetic myocytes . - pubmed
Mechanisms underlying this alteration in IGF - I - induced myocardial response may be related to changes in intracellular Ca2 + and/or NO production in diabetes . - pubmed
Thyroid hormone receptor beta - dependent expression of a potassium conductance in inner hair cells at the onset of hearing . - pubmed
dHAND and eHAND are two related basic helix - loop - helix transcription factors that are expressed in a complementary fashion in the developing right and left ventricles , respectively . - pubmed
Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p . - pubmed
We have isolated the HXT1 gene , which encodes a low - affinity , high - capacity glucose transporter , as a multicopy suppressor of a gsf2 mutation . - pubmed
gsf2 mutants also display a galactose growth defect and abnormal localization of the galactose transporter Gal2p but are not defective in function or localization of the high - affinity glucose transporter Hxt2p . - pubmed
These findings suggest that Gsf2p functions in the ER to promote the secretion of certain hexose transporters . - pubmed
Kinetic , structural and electrostatic aspects of the reduction of pentacyanoferrate ( III ) complexes by myoglobin . - pubmed
The results of this and an earlier study show that an outer - sphere mechanism is operating for electron transfer between oxymyoglobin and FeIII ( CN ) 5Ln - , independent of the lability of the ligand L. The electron transfer process is preceded by precursor formation at a specific site on the protein close to the protein heme pocket . - pubmed
Biopharmaceutical profile of cerivastatin : a novel HMG - CoA reductase inhibitor . - pubmed
Following administration , cerivastatin was rapidly and almost completely absorbed into the gastrointestinal tract ( & gt ; 98 % ) , with maximum plasma concentrations ( Cmax ) reached at 2 - 3 h post dose . - pubmed
Vagotomy and administration of atropine abolished the gastric acid secretion induced by ghrelin . - pubmed
Hindquarters were perfused with 7 mmol / l glucose , 1,000 micromol / l albumin - bound palmitate , and albumin - bound [ 1 -( 14 ) C ] palmitate at rest ( no insulin ) . - pubmed
Modulation of Na , K - ATPase and Na - ATPase activity by phospholipids and cholesterol . - pubmed
The effects of phospholipid acyl chain length ( n(c ) ) , degree of acyl chain saturation , and cholesterol on Na , K - ATPase reconstituted into liposomes of defined lipid composition are described . - pubmed
In addition , the increased bilayer order induced by both cholesterol and saturated phospholipids could be important for the conformational mobility of the Na , K - ATPase changing the distribution of conformations . - pubmed
The temperature dependence of the Na , K - ATPase as well of the Na - ATPase reactions depends both on phospholipid acyl chain length and on cholesterol . - pubmed
Cholesterol increased significantly both the enthalpy of activation and entropy of activation for Na , K - ATPase activity and Na - ATPase activity of Na , K - ATPase reconstituted with monounsaturated phospholipids . - pubmed
In the case of ATPase reconstituted without cholesterol , the minimum free energy of activation and the maximum turnover both shifted to longer acyl chain lengths of about 22 . - pubmed
Repression of GAD autoantigen expression in pancreas beta - Cells by delivery of antisense plasmid / PEG - g - PLL complex . - pubmed
To repress GAD expression in islet beta - cells , we delivered an antisense GAD mRNA expression plasmid ( pRIP - AS - GAD ) using poly ( ethylene glycol )-grafted poly - L - lysine ( PEG - g - PLL ) as a gene carrier . - pubmed
In a gel retardation assay , the pRIP - AS - GAD / PEG - g - PLL complex was completely retarded above a weight ratio of 1:1.5 ( plasmid : PEG - g - PLL ) . - pubmed
PEG - g - PLL protected the plasmid DNA from DNase I for more than 60 minutes . - pubmed
Therefore , our study revealed that the pRIP - AS - GAD / PEG - g - PLL system is applicable for the repression of GAD autoantigen expression . - pubmed
The levels of total serum bilirubin ( P<0.05 ) and serum transaminases ( P<0.05 ) were significantly higher in patients with AIH - AH than in patients with AIH - CH and AIH - LC . - pubmed
Frizzled - related protein ( Frp ) is a new family of secreted proteins that contain a region homologous to the extracellular cysteine - rich domain ( CRD ) of the frizzled family proteins . - pubmed
The 876 - bp bla(CTX - M-17 ) gene differed from bla(CTX - M-14 ) by 2 nucleotides , which led to the single amino acid substitution Glu289 - ->Lys . - pubmed
[ Protective effect of low concentration endothelin-1 on the reactive oxygen - induced inhibition of pulmonary surfactant lipid synthesis ] . - pubmed
The effects of endothelin-1 ( ET-1 ) at low concentration ( 1 - 100 pmol / L ) on the reactive oxygen - induced inhibition of both pulmonary surfactant ( PS ) lipid synthesis and the activity of CTP : phosphorylcholine cytidylyltransferase ( CCT ) , a rate - limiting enzyme in biosynthesis of phosphoatidylcholine ( PC ) , were studied in cultured lung explants without serum . - pubmed
The xanthine - xanthine oxidase superoxide anion generating system decreased ( 3 ) H - choline incorporation into PC in a dose - dependent manner in cultured lung explants . - pubmed
ET-1 reduced both the reactive oxygen - induced decrease in ( 3 ) H - choline incorporation and the increase in malondialdehyde ( MDA ) content of lung tissues , but did not change the levels of antioxidant enzymes superoxide dismutase ( SOD ) , catalase ( CAT ) and the total antioxidant capability in the lung explants . - pubmed
ET-1 also prevented the inhibitive effect of reactive oxygen on microsomal CCT activity in the lung explants . - pubmed
The cDNA contained an open reading frame encoding 71 amino acid residues and the sequences for CdlP1 and CdlP2 were found to be located in the center of this coding region . - pubmed
The CMP - sialic acid synthetase ( CMP - Neu5Ac , synthetase ) is responsible for the synthesis of CMP - Neu5Ac , which is the donor used by sialyltransferases to attach sialic acid to acceptor hydroxyl groups in various polysaccharides , glycolipids , and glycoproteins . - pubmed
Since CMP - Neu5Ac is unstable and relatively expensive , the CMP - Neu5Ac synthetase is valuable for the preparative enzymatic synthesis of sialylated oligosaccharides . - pubmed
The purified CMP - Neu5Ac synthetase was used in the gram - scale synthesis of CMP - Neu5Ac . - pubmed
Impact of obesity and leptin levels on the secretion of estradiol , inhibin A and inhibin B during ovarian stimulation with gonadotropins . - pubmed
Serum levels of estradiol , leptin , inhibin A and inhibin B were measured . - pubmed
The results suggest that high leptin concentrations in vivo are not associated with impaired secretion of estradiol and dimeric inhibins during ovarian stimulation . - pubmed
Here we report that ura4 + , encoding orotidine monophosphate decarboxylase ( OMPdecase ) , shows glucose - inducible expression regulated at post - transcriptional level . - pubmed
The ura4 + mRNA level was rapidly decreased by approximately 50 % within 20 min after glucose depletion and it was readily recovered upon glucose - readdition within 1 h. Glucose at above 2 % similarly raised the transcript level of ura4 + , while low concentration ( 0.1 % ) was not effective . - pubmed
Interestingly , control of mRNA turnover would be the main regulatory step of the glucose - dependent expression of ura4 + . - pubmed
These results suggest that the SAPK pathway might participate in the glucose - dependent regulation of ura4 + and rrg1 + mRNA stabilities . - pubmed
As concerns follow - up of the CR patients , one did not receive any further treatment and died in CR from pulmonary aspergillosis , and one with a concomitant chronic lymphocytic leukemia ( CLL ) received two courses of FLAG ( fludarabine , cytarabine , granulocyte colony - stimulating factor ) regimen with disappearance of the clonal Ig rearrangement , but relapsed after 11 months and died from pneumonia . - pubmed
Analysis of uterine oestrogen receptor alpha revealed that naringenin significantly increased the cytosolic concentration of oestrogen receptor alpha , whereas in nuclei the oestrogen receptor alpha concentration was significantly decreased as compared to the solvent control . - pubmed
This was in contrast to the positive control 17 beta - oestradiolacetate which acted as a true oestrogen by increasing the concentration of both total and nuclear oestrogen receptor alpha . - pubmed
Both naringenin and 17 beta - oestradiolacetate , however , significantly , induced nuclear oestrogen receptor alpha in the liver , suggesting a tissue specific effect of naringenin on oestrogen receptor alpha distribution . - pubmed
Similarly , exposure to glutamate or staurosporine induced significantly more neuronal cell death in galanin knockout organotypic and dispersed primary hippocampal cultures than in WT controls . - pubmed
Further , exogenous galanin or the previously described high - affinity GALR2 agonist , both reduced cell death when coadministered with glutamate or staurosporine in WT cultures . - pubmed
The HER-2 - targeting antibodies trastuzumab and pertuzumab synergistically inhibit the survival of breast cancer cells . - pubmed
Trastuzumab ( herceptin ) and pertuzumab ( Omnitarg , 2C4 ) are recombinant humanized monoclonal antibodies that target different extracellular regions of the HER-2 tyrosine kinase receptor . - pubmed
Infrared ( IR ) spectroscopy using a smart endurance single bounce diamond attenuated total reflection ( ATR ) cell has been used to study the changes in the spectra of the surfactant octadecyltrimethylammonium ( ODTMA ) bromide upon intercalation into a sodium montmorillonite . - pubmed
Using a phosphospecific antibody to Syk ( Tyr525 / Tyr526 ) that measures activated Syk , we found that most ( 60%-75 % ) of the active Syk is in the ubiquitinated fraction . - pubmed
The role of N - glycosylation in the stability , trafficking and GABA - uptake of GABA - transporter 1 . - pubmed
Terminal N - glycans facilitate efficient GABA - uptake activity of the GABA transporter . - pubmed
The GABA transporter GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins which possess 12 putative transmembrane domains and three N - glycosylation sites in the extracellular loop between transmembrane domain 3 and 4 . - pubmed
This indicates that N - glycans , in particular their terminal trimming , are important for the GABA - uptake activity of GAT1 . - pubmed
For example , the ATP - binding cassette ( ABC ) drug transporters have been shown to protect cancer stem cells from chemotherapeutic agents . - pubmed
The structures of N - ethyl-3 -( 4 - fluorophenyl )- 5 -( 4 - methoxyphenyl )- 2 - pyrazoline-1 - thiocarboxamide , C19H20FN3OS , ( I ) , and 3 -( 4 - fluorophenyl )- N - methyl-5 -( 4 - methylphenyl )- 2 - pyrazoline-1 - thiocarboxamide , C18H18FN3S , ( II ) , have similar geometric parameters . - pubmed
The methoxy / methyl - substituted phenyl groups are almost perpendicular to the pyrazoline ( pyraz ) ring [ interplanar angles of 89.29 ( 8) and 80.39 ( 10 ) degrees for ( I ) and ( II ) , respectively ] , which is coplanar with the fluorophenyl ring [ interplanar angles of 5.72 ( 9 ) and 10.48 ( 10 ) degrees ] . - pubmed
We investigated the bioavailability of DSM Nutritional Products Ltd. ( Kaiseraugst , Switzerland ) CoQ10 10 % TG / P ( all - Q ) , a new tablet - grade formulation , with CoQ10 Q - Gel Softsules based on the Bio - Solv technology ( Tishcon Corp. , Salisbury , MD ; marketed by Epic4Health , Smithtown , NY ) and Q - SorB ( Nature 's Bounty , Bohemia , NY ) . - pubmed
The disease - modifying treatments , IFN - beta and glatiramer acetate , have been widely available over the last decade and have shown a beneficial effect on relapse rate and magnetic resonance imaging parameters of disease activity ; however , their effect on disease progression and disability is modest . - pubmed
These include humanized monoclonal antibodies such as daclizumab ( IL-2 antagonist ) , oral immunomodulators such as sirolimus and statins and neuroprotective agents such as NMDA antagonists and Na +- channel blockers . - pubmed
Overall Tween-20 extracts effectively decreased levels of TNF - alpha , IL-1 , IL-2 and IL-6 as observed using cytokine bioassays . - pubmed
Tween-20 extracts effectively inhibited both COX-1 and COX-2 cyclooxygenase activity . - pubmed
Although the anti - cytokine activity of the Tween-20 extract was destroyed by Proteinase - K treatment , the anti - COX-1 and anti - COX-2 activity of both the extracts were not sensitive to protease treatment . - pubmed
Surgical specimens were sliced transversely on serial embedded blocks at 2.5 - mm intervals , and stained with hematoxylin and eosin ( HE ) . - pubmed
Tumor - suspect nodules previously considered disease free by HE stain on whole - mount section were examined by in situ hybridization ( ISH ) on tissue microarray ( TMA ) through detecting mRNAs of CEA and CK20 with non radioactive biotin - tagged oligonucleotide probes . - pubmed
Alpha - galactosidase ( alpha - Gal ) enzyme , which is encoded by the melA gene hydrolyzes alpha-1,6 galactoside linkages found in sugars , such as raffinose and stachyose . - pubmed
MEASUREMENTS AND RESULTS : Four hours after LPS injection , the antioxidant enzyme activities , including superoxide dismutase ( SOD ) , glutathione peroxidase ( GPx ) , and thiobarbituric acid reactive substance ( TBARS ) levels as an indicator of lipid peroxidation , were determined . - pubmed
Intestinal apoptosis was assessed by hematoxylin - eosin staining and terminal deoxynucleotide transferase - mediated fluorescein - dUTP nick end labeling . - pubmed
Damage to ICC and pacemaking was greatly attenuated in the absence of NO derived from iNOS . - pubmed
Downregulation of c - FLIP promotes caspase - dependent JNK activation and reactive oxygen species accumulation in tumor cells . - pubmed
Nuclear factor - kappa B ( NF - kappaB ) inhibits cell death through suppression of the caspase cascade , the c - Jun N - terminal kinase ( JNK ) pathway , and reactive oxygen species ( ROS ) accumulation . - pubmed
New studies support this idea by showing that the catalysis of vitamin A into retinoic acid ( RA ) in gut - associated dendritic cells ( DCs ) enhances the transforming growth factor ( TGF )- beta - dependent conversion of naive T cells into regulatory T ( T reg ) cells and also directs T reg cell homing to the gut . - pubmed
Binding of dioxygen to a non - heme enzyme has been modeled using the ONIOM combined quantum mechanical / molecular mechanical ( QM / MM ) method . - pubmed
For the present system , isopenicillin N synthase ( IPNS ) , binding of dioxygen is stabilized by 8 - 10 kcal / mol for a QM : MM ( B3LYP : Amber ) protein model compared to a quantum mechanical model of the active site only . - pubmed
In the protein system , the free energy change of O2 binding is close to zero . - pubmed
The second major factor that stabilizes O2 binding is van der Waals interactions between dioxygen and the surrounding enzyme . - pubmed
Both GF and CL expression are highly induced at the onset of fruit ripening , coincident with the ripening - associated decline in chlorophyll . - pubmed
The SGR subfamily is required for chlorophyll degradation and operates through an unknown mechanism . - pubmed
Levels of fasting total - C , HDL - C , LDL - C , triglycerides , homocysteine , and lipoprotein ( a ) were measured . - pubmed
After 6 months of the therapy , serum levels of total - C , LDL - C , and homocysteine decreased significantly ( P=.04 , P=.02 , P=.008 , respectively ) in the treated group when compared with the control group . - pubmed
Moreover , deletion of N - terminus , proximal acidic domain or , surprisingly , zinc finger domains leads to Rpn4p stabilization . - pubmed
We found that the non - selective NOS inhibitor 7 - nitroindazole ( 7 - NI ) as well as the more selective neuronal and inducible NOS inhibitor 1 -( 2 - trifluoromethylphenyl ) imidazole ( TRIM ) decreased the number of marbles buried . - pubmed
Inhibition of nNOS seems to play a key role in the behavioral effects of NOS inhibitors , whereas agmatine seems to have a different mechanism of action . - pubmed
Nevertheless , exposure to the environmental xenobiotic 2,3,7,8 - tetrachlorodibenzo - p - dioxin ( TCDD ) , a well - known exogenous ligand of the Ahr pathway , led to overexpression of the Ahr target gene Cyp1a1 , and to increased sensitivity to TCDD - triggered apoptosis in E2F2(-/- ) T cells compared with WT controls . - pubmed
An artificial model of photosynthetic photosystem II : visible - light - derived O2 production from water by a di - mu - oxo - bridged manganese dimer as an oxygen evolving center . - pubmed
Visible - light - derived O ( 2 ) production was yielded by conjugating water oxidation catalysis by [ ( OH ( 2 ))( terpy ) Mn ( mu - O )( 2 ) Mn ( terpy )( OH ( 2 ))]( 3 + ) as an oxygen evolving center model and photo - sensitization of [ Ru ( bpy )( 3 )]( 2 + ) as a photoexcitation center model at an interlayer of mica . - pubmed
On 23 October 2009 , the commissioner of the US Food and Drug Administration ( FDA ) issued an Emergency Use Authorization ( EUA ) for peramivir for intravenous injection , an unapproved neuraminidase inhibitor used for treating certain hospitalized adult and pediatric patients infected with 2009 H1N1 influenza . - pubmed
METHODOLOGY / PRINCIPAL FINDINGS : We tested the possibility that L - DOPA might interfere with DAT binding . - pubmed
CONCLUSIONS / SIGNIFICANCE : These data indicate that L - DOPA does not induce modifications of DAT expression detectable by SPECT of by DAT binding autoradiography , suggesting that differences between clinical assessment and radiotracer imaging in clinical trials may not be specifically related to L - DOPA treatment . - pubmed
Expression of drug - transporting P - glycoprotein ( P - gp , an integral protein of the plasma membrane ) in neoplastic cells confers multidrug resistance and also involves alteration of cell sensitivity to inhibitors of the sarco / endoplasmic reticulum calcium pump thapsigargin ( Th ) . - pubmed
Purification and characterization of cystathionine beta - synthase bearing a cobalt protoporphyrin . - pubmed
Metal content analysis showed that the enzyme contained 92 % cobalt and 8 % iron . - pubmed
CoCBS was indistinguishable from wild - type FeCBS in its activity , tetrameric oligomerization , PLP saturation and responsiveness to the allosteric activator , S - adenosyl - l - methionine . - pubmed
The coordinators of these processes may be small GTPases of the Rho family and their activators , the factors of exchange of guanylic nucleotides . - pubmed
Decreased RRP22 expression levels were in part explained by 5'-CpG island hypermethylation and increased by the treatment with the demethylating agent 5 - aza-2 '- deoxycytidine in glioblastoma cell lines . - pubmed
In addition , the in vitro treatment with the histone deacetylase inhibitor trichostatin A alone resulted in RRP22 reexpression as well as a significant increase in the levels of RRP22 promoter DNA bound to pan - acetylated histone H3 and H4 . - pubmed
In a laboratory study , formulations of 15 % ( w / w ) of ordinary Portland cement ( OPC ) , calcium aluminate cement ( CAC ) and pozzolanic cement ( PC ) and additives : plasticizers cementol delta ekstra ( PCDE ) and cementol antikorodin ( PCA ) , polypropylene fibers ( PPF ) , polyoxyethylene - sorbitan monooleate ( Tween 80 ) and aqueous acrylic polymer dispersion ( Akrimal ) were used for solidification / stabilization ( S / S ) of soils from an industrial brownfield contaminated with up to 157 , 32,175 , 44,074 , 7614 , 253 and 7085 mg kg(-1 ) of Cd , Pb , Zn , Cu , Ni and As , respectively . - pubmed
The THG experiments for type I and type II LCB crystals were performed . - pubmed
Thin films of nickel and lanthanum - strontium - manganite ( LSM ) are grown on silicon substrate at 573 K using selected metallorganic complexes of Ni [( acac ) 2en ] , La ( tmhd ) 3 , Sr ( tmhd ) 2 and Mn ( tmhd ) 3 as precursors by plasma assisted liquid injection chemical vapor deposition ( PA - LICVD ) . - pubmed
Serum alanine aminotransferase , aspartate aminotransferase , gamma - glutamyl , alkaline phosphatase , bilirubin , and albumin levels , presentation patterns , and radiologic findings were assessed . - pubmed
The basal [ ( 35 ) S ] GTPgammaS binding was changed by varying the GDP concentration whilst the basal F - actin contents of the higher expressing T cell populations were elevated , suggesting constitutive activity of CCR4 . - pubmed
RESULTS The affinity of CCL17 ( also known as TARC ) derived from analysis of the T cell data ( pK(a ) = 9.61 + - 0.17 ) was consistent with radioligand binding experiments ( 9.50 + - 0.11 ) while that from the [ ( 35 ) S ] GTPgammaS binding experiments was lower ( 8.27 + - 0.09 ) . - pubmed
For this reason an LC - MS - MS method was developed for detection of wide range of NSAIDs , including both " acidic " NSAIDs ( carprofen , diclofenac , flunixin , meloxicam , phenylbutazone , oxyphenbutazone , tolfenamic acid , mefenamic acid , naproxen , ketoprofen , ibuprofen , firocoxib , rofecoxib , and celecoxib ) and " basic " NSAIDs ( four metamizole metabolites ) . - pubmed
All NSAIDs were separated on a Phenomenex Luna C8(2 ) column and analyzed by LC - MS - MS in negative ( acidic NSAIDs ) and positive ( metamizole metabolites ) ion modes . - pubmed
OsbZIP16 was shown to be induced by exogenous ABA treatment , while overexpression of OsbZIP16 was observed to make transgenic plants more sensitive to ABA than wild type plants were . - pubmed
Intracerebroventricular injections of 6 - OHDA induced a bilateral loss of tyrosine hydroxylase - positive cells in the substantia nigra ( -51 % ) and ventral tegmental area ( -31 % ) . - pubmed
Sequence analysis has shown that Spy0843 contains two leucine - rich repeat ( LRR ) domains that mediate interactions with the gp340 receptor . - pubmed
Elucidation of the crystal structure will provide insights into the interactions of Spy0843 with the gp340 receptor and a better understanding of the role of Spy0843 in streptococcal infections . - pubmed
The abundance of cysteines in this isoform is particularly fascinating , as hVDAC-1 cysteines have no associated functional role . - pubmed
We report a detailed biophysical examination of a Cys - less mutant of hVDAC-2 , and its behavioral comparison with the wild type protein . - pubmed
These findings suggest that HyaB and HydA can function as uptake hydrogenases that couple the oxidation of H2 to the reduction of amaranth to sustain cellular growth . - pubmed
Assays with respiratory inhibitors indicated that a menaquinone pool and different cytochromes were involved in the azoreduction process . - pubmed
The fact that certain MHC - I receptors have the unusual capacity to recognize MHC - I molecules expressed by other cells ( trans ) and by the NK cell itself ( cis ) has raised the question regarding possible contributions of the two types of interactions to NK cell education . - pubmed
OBJECTIVE : Evaluate ustekinumab , an anti - interleukin ( IL )- 12 and IL-23 antibody , effects on radiographic progression in psoriatic arthritis ( PsA ) . - pubmed
Treatment included an insulin infusion , omega-3 fatty acid esters , and gemfibrozil . - pubmed
The insulin infusion reduced serum triglyceride levels by 50 % after 1 day of treatment and to 355 mg / dL by day 7 of her ICU course . - pubmed
Specimens were randomly assigned to five groups according to the disinfectant used : Group I : Control ( no disinfectant ) ; Group II : 5.25 % sodium hypochlorite based ; Group III : 2 % chlorhexidine based ( Consepsis ) , Group IV : 0.1 % benzalkoniumchloride based ( Tubulicid red ) and Group V : 3 % doxycycline based ( Biopure , MTAD ) . - pubmed
RESULTS : Dentin disinfectants tested significantly negated the bonding of Adper Single bond 2 and the groups were ranked ; Group I & gt ; Group V = Group IV & gt ; Group II = Group III , meanwhile they enhanced significantly the mu - SBS values upon using Clearfil S ( 3 ) Bond and were ranked ; Group II & gt ; Group III = Group IV = Group V & gt ; Group I. - pubmed
Efficacy of some non - conventional herbal medications ( sulforaphane , tanshinone IIA , and tetramethylpyrazine ) in inducing neuroprotection in comparison with interleukin-10 after spinal cord injury : A meta - analysis . - pubmed
OBJECTIVES : To assess the efficacy of some non - conventional herbal medications including sulforaphane , tanshinone IIA , and tetramethylpyrazine in reducing inflammation and compare them with a known effective anti - inflammatory agent ( interleukin-10 ( IL-10 ) ) . - pubmed
Tetramethylpyrazine has an acceptable influence in reducing inflammation through the up - regulation of IL-10 . - pubmed
Outcomes of sulforaphane and tanshinone IIA administration are acceptable but still weaker than IL-10 . - pubmed
AIM : Hip arthrography usually requires the injection of iodine based dyes which can cause complications . - pubmed
In addition , anti - cancer activities were confirmed by conducting MTT assay for ART and ART - CLN on different cancer cell lines . - pubmed
Glucagon - like peptide-1 ( GLP-1 ) is a metabolic hormone that is secreted in a glucose - dependent manner and enhances insulin secretion . - pubmed
The results suggest that exendin-4 potentiates a subpopulation of extrasynaptic GABAA receptors in the CA3 pyramidal neurons . - pubmed
Phosphorylation of the transcription factor cAMP - responsive element binding protein ( CREB ) significantly increased in cells treated with high glucose ( HG ) compared to cell grown in normal glucose ( NG ) . - pubmed
Cells pretreated with rapamycin before exposure to HG showed significant decrease phosphorylation of CREB , increase in AMPK activity and decrease protein / mRNA and promoter activity of fibronectin . - pubmed
Cells treated with HG showed nuclear localization of p - CREB while pretreated cells with rapamycin reversed HG effect . - pubmed
Moreover , gel shift analysis shows increase binding of CREB to fibronectin promoter in cells treated with HG while cells pretreated with rapamycin reversed the effect of HG . - pubmed
Excessive accumulation of histamine in the body leads to miscellaneous symptoms mediated by its bond to corresponding receptors ( H1 - H4 ) . - pubmed
HOXA13 decreased beta - catenin , phospho - smad2 , and phospho - smad3 in the nucleus and increased phospho - beta - catenin in the cytoplasm . - pubmed
RASD1 has been shown to inhibit adenylyl cyclase activity in vitro resulting in the inhibition of the cAMP - PKA - CREB signaling pathway . - pubmed
Therefore , we tested the hypothesis that RASD1 may inhibit cAMP stimulated gene expression in the brain . - pubmed
RESULTS : We show that Rasd1 is expressed in vasopressin neurons of the PVN and SON , within which mRNA levels are induced by hyperosmotic cues . - pubmed
These effects were dependent upon isoprenylation , as both farnesyltransferase inhibitor FTI-277 and CAAX box deletion prevented Rasd1 inhibition of cAMP - induced gene expression . - pubmed
We propose that the abundance of RASD1 in vasopressin expressing neurons , based on its inhibitory actions on CREB phosphorylation , is an important mechanism for controlling the transcriptional responses to stressors in both the PVN and SON . - pubmed
These effects likely occur through modulation of cAMP - PKA - CREB signaling pathway in the brain . - pubmed
The enzyme was completely inhibited by PMSF and significantly inhibited by EDTA , TPCK , Co ( 2 + ) , Zn ( 2 + ) , and Cu ( 2 + ) , suggesting a chymotrypsin - like serine metalloprotease . - pubmed
On post injury day ( PID ) 1 , 2 , 3 , the vital signs , volume of fluid input , and volume of the urine output were observed and recorded ; femoral artery blood was drawn to determine lactate , bicarbonate radical , and base excess , and oxygen index was calculated . - pubmed
At PIH 12 , 24 , 48 , 72 , femoral vein blood was drawn to determine white cell count , platelet count , neutrophils , creatine kinase - MB , creatine kinase , lactic dehydrogenase , aspartate transaminase ( AST ) , alanine aminotransferase ( ALT ) , creatinine , urea nitrogen , and blood glucose ( the ratio of AST to ALT was calculated ) . - pubmed
Superelastic ( SE ) and thermo - activated ( TA ) nickel - titanium ( NiTi ) archwires are used in everyday orthodontic practice , based on their acceptable biocompatibility and well - defined shape memory properties . - pubmed
The cytotoxic effect of Rematitan SE correlated with the higher release of Ni ions in CM , higher concentration of surface Ni and an increased oxygen layer thickness after the conditioning . - pubmed
In this protocol , the quantitative derivation of the spring - constant and drag - coefficient of the bond between adhering colloidal particles and substratum surfaces using QCM - D is explained for bacteria and silica particles , using the particle - mass derived for validation . - pubmed
Dopamine D2 receptors mediate the increase in reinstatement of the conditioned rewarding effects of cocaine induced by acute social defeat . - pubmed
In the present study we evaluated the effect of the dopamine D1 - and D2 - like receptor antagonists ( SCH23390 and raclopride , respectively ) on the short - time effects of acute social defeat ( ASD ) . - pubmed
A final experiment was designed to evaluate the effect of the dopamine antagonists on the CPP induced by 3mg / kg of cocaine with or without a stress experience . - pubmed
Blockade of dopamine D2 - like receptors with raclopride specifically prevented the effects of stress without affecting the rewarding properties of cocaine . - pubmed
Our results confirm that the dopamine D2 receptor is involved in the short - term effects of ASD on the rewarding effects of cocaine . - pubmed
The dopamine D1 receptor is clearly involved in the rewarding effects of cocaine , but its role in the effects of ASD remains to be demonstrated . - pubmed
Hierarchical regression was used to evaluate if gratitude , social support , and satisfaction with life moderated the relationship between stressful life events ( as measured by the total life stress score ) and PTG , after adjustment for age , sex , race , level of involvement in Hurricane Katrina , and alcohol intake . - pubmed
Imatinib treatment not only prevented ankle swelling and synovial inflammation when administered before MSU crystals but also diminished these features when administrated after the injection of MSU crystals , a therapeutic protocol more closely mimicking the clinical situation in which treatment occurs after the development of an acute gout flare . - pubmed
A simple , mild and reproducible synthetic route was developed to fabricate the multifunctional Au@carbon / calcium phosphate core - shell nanoparticles ( Au@C / CaP NPs ) , which act as an efficient nanoplatform for pH / near - infrared ( NIR ) dual - responsive drug delivery , X - ray computed tomography ( CT ) imaging and synergistic chemo - photothermal cancer therapy in vitro . - pubmed
Deciphering protein dynamics changes along the pathway of retinol uptake by cellular retinol - binding proteins 1 and 2 . - pubmed
CRBP 1 and 2 , sharing a 56 % amino acid sequence identity , exhibit the highest binding affinities for retinol . - pubmed
Herein , the results of molecular dynamics simulations for the uptake of retinol by CRBP 1 and 2 are consistent with the presence of two different retinol entry points , both involving the ' cap region ' ( alpha - helices I and II and neighboring loops ) . - pubmed
We observed that a hydrophobic patch at the surface of the ' portal region ' ( alpha - helix II , CD and EF loops ) of CRBP 1 attracts retinol , which accesses the binding cavity through an opening generated by the concerted movements of Arg58 and Phe57 , present in the CD loop . - pubmed
In CRBP 2 a different distribution of the surface residues of the ' cap region ' allows retinol to access the binding cavity by sinking in a hydrophobic matrix between the two alpha - helices . - pubmed
Polar interactions mainly affect retinol movements inside the beta - barrel cavities of both CRBPs . - pubmed
More valuable information could probably be provided about the variety of the diseases caused by superoxide anions toxicities by intervention and application of the non - viral method for expressions of SOD1 and SOD3 enzymes . - pubmed
The identified 20 - amino acid sequence of the purified peak by Edman degradation shared 100 % identity with the N - terminal regions of mytichitin-1 , mytichitin-2 , mytichitin-3 , mytichitin-4 , mytichitin-5 , and chitinase - like protein-1 , and so was named mytichitin - CBD . - pubmed
An opioid modulator is a drug that can produce both agonistic and antagonistic effects by binding to different opioid receptors and therefore can not be classified as one or the other alone . - pubmed
In summary , the structural elucidation of opioid receptors , allosteric modulation of opioid receptors , new opioid modulators and agonists , the employment of optogenetics , optopharmacology , and next - generation sequencing of opioid receptor genes and related functionality should create exciting new avenues for research and therapeutic development to treat conditions including pain , opioid abuse , and addiction . - pubmed
Further targeted metabolomics by GC - MS uncovered the identity of the deoxyhexose with 1,5 - anhydroglucitol displaying the most significant changes . - pubmed
Fatty acids ( FA ) are crucial in this process , since they have a dual role as energetic coins and building blocks . - pubmed
In vitro , we verified that FA and estradiol stimulated FATP1 / SLC27A1 expression in BCCs . - pubmed
Additionally , experiments with estradiol and PHTPP ( ER - beta antagonist ) showed that estrogen receptor - beta ( ER - beta ) regulates FATP1 / SLC27A1 expression , the uptake of FA and cell viability , in four BCC lines . - pubmed
Furthermore , the inhibition of FATP1 with arylpiperazine 5k ( DS22420314 ) interfered with the uptake of FA and cell viability . - pubmed
BACKGROUND : Fingolimod ( FTY ) applied as treatment regimen of relapsing - remitting multiple sclerosis ( RRMS ) induces downregulation of sphingosine-1 - phosphate receptors on the lymphocytes . - pubmed
Hereby , we report a 71 - year - old female with seronegative rheumatoid arthritis who presented with massive splenomegaly , pancytopenia , and positivization of antibodies against double - stranded deoxyribonucleic acid ( dsDNA ) after initiation of the anti - tumor necrosis factor ( TNF ) golimumab . - pubmed
Withdrawal of the anti - TNF and treatment with the anti - CD20 antibody rituximab were able to revert the clinical abnormalities . - pubmed
In this paper , we propose a surface plasmon resonance ( SPR ) sensor based on two - dimensional ( 2D ) materials ( graphene , MoS2 , WS2 and WSe2 ) hybrid structure , and theoretically investigate its sensitivity improvement in the visible region . - pubmed
An Ag - based SPR sensor exhibits a higher sensitivity than an Au- or Cu - based one at most wavelengths in the visible region . - pubmed
In addition , the sensitivity of the proposed SPR sensor varies obviously with the wavelength of incident light , and shows a maximum value of 159 , 194 or 155 /RIU for Au , Ag or Cu , respectively . - pubmed
Not surprisingly , direct visualization of RGCs by SLO fundus imaging correlated best with histological quantification of RGC somata and axons . - pubmed
Using gas chromatography - mass spectrometry ( GC - MS ) , we identified methyl palmitate and methyl stearate as bioactive compounds . - pubmed
The effects of OMSW different pretreatments ( microwave or autoclave ) , different additives ( water , formic , or sulfuric acid ) , and utilization of different saccharification enzymes ( Cellic CTec2 or Accellerase 1500 ) were tested on saccharification process and sugar release , and on fermentation inhibitors formation and ethanol production . - pubmed
Simplified LC - MS Method for Analysis of Sterols in Biological Samples . - pubmed
The method was validated on 10 sterol standards that were detected in a single chromatographic LC - MS run without derivatization . - pubmed
The developed method enables a simple , sensitive , and fast quantification of sterols , without the need of extended knowledge of the LC - MS technique , and represents a new analytical tool in the rising field of cholesterolomics . - pubmed
Multivariate analysis showed that baseline steroids was an independent predictor for a worse OS ( P<0.001 ) , while performance status ( PS)>=2 was an independent predictor for a poorer TTF ( P<0.001 ) and OS ( P<0.001 ) . - pubmed
How Oxygen - binding Enhances Long - range Electron Transfer : Lessons from Reduction of Lytic Polysaccharide Monooxygenases by Cellobiose Dehydrogenase . - pubmed
Using multiscale simulations , we reveal that interprotein ET between CDH and LPMO is mediated by the heme propionates of CDH and solvent waters . - pubmed
Interestingly , we find that oxygen binding to the copper center of LPMO is coupled with the long - range interprotein ET . - pubmed
The uncovered ET mechanism rationalizes experimental observations and has far - reaching implications in LPMO catalysis , as well as the O 2 or CO binding - enhanced long - range ET processes in other metalloenzymes . - pubmed
However , inhibiting KATP by 5 - HD and Gly significantly reversed the effects , including NLRP3 inflammasome and inflammatory mediators IL-6 , MPO , TNF - alpha , and MCP-1 . - pubmed
Stimulation of glucose oxidation by insulin is reduced in large cells . - pubmed
Studies using a mimicker of insulin action , spermine , as well as measurements of 125I - insulin binding to large and small cells indicate that receptor number and affinity are not responsible for hormone resistance . - pubmed
Furthermore , when rapid and direct measurements of sugar uptake were made , insulin stimulation was virtually identical in both cell types . - pubmed
These findings indicate that large adipocytes have an efficient insulin - responsive D - glucose transport system and suggest that the apparent hormone resistance may be due to alterations in intracellular glucose metabolism . - pubmed
Microsomal mixed - function oxidases and epoxide hydratase convert benzo ( a ) pyrene stereospecifically to optically active dihydroxydihydrobenzo ( a ) pyrenes . - pubmed
After induction by TCB , EROD was detectable in microsomes from whole thymus , bursa and in peritoneal exudate cells ( containing primarily macrophages ) at levels of 28.3 , 7.2 and 1.3 pmol / mg microsomal protein / min , respectively ; the level in control liver was 89.9 pmol / mg microsomal protein / min . - pubmed
The P450I specific in vitro inhibitor , alpha - naphthoflavone ( NF ) inhibited the TCB - induced liver and immune tissue EROD by 50 % at concentrations in the range of 0.07 - 0.1 microM. - pubmed
Much higher TCB - induced EROD was detected in immune tissue supporting cells than in lymphocytes , particularly in the thymus . - pubmed
TCB - induced EROD was 12 - fold higher in the lymphocyte - depleted thymus than in normal thymus , with a less marked but similar pattern in the bursa. - pubmed
Previously it was shown [ 1 ] that amino acid substitutions at the region of the first alpha - helix of IL-2 specifically inactivate its reactivity with the intermediate - affinity receptor p70 , and mutations in the fifth alpha - helix specifically inactivate the binding to the low - affinity receptor p55 . - pubmed
We have synthesized the peptides corresponding to the putative binding site of IL-2 with the intermediate - affinity receptor p70 and found that the nonapeptide corresponding to the sequence 27 - 35 of the mature IL-2 [ 2 ] effectively competes with human rIL-2 for binding to thymocyte receptors . - pubmed
Characterization of the aluminum and beryllium fluoride species which activate transducin . - pubmed
Aluminofluoride and beryllofluoride complexes can activate the heterotrimeric G - proteins by binding next to GDP in the nucleotide site of their G alpha subunit and acting as analogs of the gamma - phosphate of a GTP . - pubmed
We have studied , by monitoring the activation - dependent tryptophan fluorescence of transducin T alpha subunit , the pF ( -log[F- ] ) and pH dependencies of the kinetics of activation and deactivation of T alpha GDP in the presence of NaF and aluminum or beryllium salts . - pubmed
After 2 weeks , the fish were exposed to severe environmental hypoxia ( final water oxygen partial pressure , PWO2 = 25 - 45 Torr , reached within 20 min ) , to assess the impact of the experimentally induced alkalosis , and hence increased haemoglobin - oxygen ( Hb - O2 ) affinity , on various aspects of the hypoxic response . - pubmed
This was accomplished by monitoring oxygen partial pressure and total oxygen content of arterial blood ( PaO2 and CaO2 ) , extracellular pH ( pHe ) , red blood cell ( RBC ) intracellular pH ( pHi ) , and the concentrations of plasma adrenaline , noradrenaline , lactate and haemoglobin ( Hb ) at 5 min intervals . - pubmed
Determination of the PaO2 thresholds for lactate and catecholamine release demonstrated that these substances appeared in the bloodstream when the degree of O2 saturation of the haemoglobin fell below 60 % . - pubmed
Cytochrome c , an extrinsic protein , at a 10:1 mole ratio , does not modify the phase - dependent behaviour of the terminal methyl groups , but consistently shifts all the observed bands to lower - frequency positions , which suggests a long - range effect of the protein along the phospholipid fatty acyl chains . - pubmed
Nonpeptidic angiotensin II antagonists : synthesis and in vitro activity of a series of novel naphthalene and tetrahydronaphthalene derivatives . - pubmed
Starting from the structure of the novel nonpeptidic angiotensin II antagonist DuP 753 , a series of more rigid analogues was prepared by replacing the biphenyl part of DuP 753 with a naphthalene ring . - pubmed
These results may indicate that the compounds do not bind to the AT1 receptor in the same way as DuP 753 . - pubmed
We conclude that ( 1 ) rIFN - alpha does not have a significant leukaemia - specific effect on the progenitor cells detected in these assays , and ( 2 ) the sensitivity of CML GM - CFC to rIFN - alpha is independent of the type of BCR / ABL message present in the cells . - pubmed
Endoglycosidase F incubated with C5 followed by monosaccharide analyses by anion exchange chromatography with pulsed amperometric detection revealed both high mannose and complex ( terminate in SA ) oligosaccharides were hydrolyzed ; no effect was found on the functional activity of C5 . - pubmed
Approximately 4 % of the complex oligosaccharides were hydrolyzed from C5 . - pubmed
Of the sialidase - treated 125I - C5 15 % was bound to EAC1gp(4b , oxy2a,3b)hu compared to 9.3 % of buffer - treated 125I - C5 . - pubmed
Furthermore , 27 % of sialidase - treated 125I - C5 was bound to EAC1gp,4bhu compared to 16.6 % of buffer - treated 125I - C5 , but no lysis occurred after the addition of C6 - C9 . - pubmed
A new approach to determine the stereospecificity in lipase catalysed hydrolysis using circular dichroism ( CD ): lipases produce optically active diglycerides from achiral triglycerides . - pubmed
We describe a sensitive CD method for determining the stereospecificity in lipase ( E.C.3.1.1.3 ) catalysed hydrolysis of triacyl glycerols into diacyl glycerols . - pubmed
The diglycerols were converted to chiral tert - butyldimethylsilylated 1,2 - or 2,3 - di - O - benzoyl - sn - glycerol ( 5 or 5 ' ) , and their CD was measured . - pubmed
This approach showed for the first time that lipases produce optically active diacyl glycerides from achiral tripalmitin and tribenzoyl glyceride with a variable extent of enantioselectivity depending on the acyl groups and the enzymes . - pubmed
[ Post - treatment evaluation of fillings with Cermet cement ( Ketac - Silver ) ] . - pubmed
Rates of total glucose utilization , glucose oxidation , nonoxidative glucose disposal ( NOGD ) , glucose metabolic clearance rate ( MCR ) , and endogenous glucose production ( EGP ) were determined with a " two - level " insulin - clamp technique ( 100 - min infusions at 40 and 400 mU X m-2 X min-1 ) combined with indirect calorimetry and D-3 -[ 3H ] glucose infusion . - pubmed
The role of thiols in lymphocyte responses : effect of 2 - mercaptoethanol on interleukin 2 production . - pubmed
Since 2 - ME could conceivably affect IL2 production and utilization equally , resulting in no apparent change in the IL2 concentration of treated cultures , 12 - h cultures were used to minimize any effects of IL2 utilization on the IL2 concentrations observed . - pubmed
No effect was observed on IL2 concentrations at 12h when cultures were treated with 2 - ME under conditions in which the 2 - ME enhanced cellular activation / proliferation . - pubmed
On the basis of plasma cotinine determination all real smokers had higher 7 - ethoxyresorufin 0 - deethylase ( ERDE ) activities measured either in placental microsomes or liver biopsy homogenates than non - smokers . - pubmed
Classification based on plasma cotinine levels showed a statistically significant ( P less than 0.001 ) difference between smokers and non - smokers in liver homogenate ERDE activity . - pubmed
The above studies showed that 3 drugs : ribamydil ( 50 micrograms / ml ) , phosphonoformate ( 120 micrograms / ml ) and recombinant interferon ( 1000 IU / ml ) were capable of statistically significant inhibition of retrovirus replication in continuous cultures of T - lymphocytes . - pubmed
However , the optimal dose levels were lower and the effective dose interval more restricted as compared to the previously described poly [ Lys -( Leui - DL - Alam ) ] ( LAK ) . - pubmed
Like LAK , both HAK and D - LAK were able to compensate for the immunosuppressive effect of the cytotoxic drugs dianhydrogalactitol , vincristine , and 5 - fluorouracil , which all have different mechanisms of action , provided that combined treatment by polypeptide and drug was applied repeatedly before the SRBC immunization . - pubmed
Clinical grade heparin is a very heterogeneous mucopolysaccharide , containing molecules with Mr ranging from 6,000 to 30,000 that have either a high affinity or a low affinity for antithrombin III ( AT ) . - pubmed
Equivalent amounts ( carbohydrate moiety ) of covalent complexes of heparin or of both heparin fragments with human AT resulted in an antithrombotic effect lasting for 45 - 60 min . - pubmed
Both [ 3H ] proline and [ 3 ] fucose were transported transneuronally to the ipsilateral abducens nucleus ( AN ) ; with long survivals [ 3H]proline was transported peripherally via the ipsilateral abducens nerve root . - pubmed
Photooxidation of lanthanide ion - lysozyme complexes . - pubmed
Basal adenylate cyclase activity in the testicular membrane fraction increased 20 to 30 % above control in response to gossypol administration . - pubmed
Testis membranes from the 20- and 30 - mg treatment group exhibited a 2- and 4 - fold greater activation of adenylate cyclase by guanine nucleotides . - pubmed
In vitro dose - response curves showed a half - maximal inhibitory concentration ( IC50 ) for inhibition of soluble testicular adenylate cyclase by gossypol of 400 microM in each treatment group . - pubmed
During synaptosomal reduction , the electron paramagnetic resonance ( EPR ) high field line of DMPO - OH is broadened indicating restrictions on the motion of the nitroxide . - pubmed
This was not due to simple absorption of DMPO - OH to proteins . - pubmed
Under these conditions , the average glucose infusion rate ( M ) is an index of glucose metabolic rate , the average insulin response ( I ) is an index of beta cell responsiveness to hyperglycemia , and the M / I ratio is an index of insulin sensitivity . - pubmed
The biochemical profile [ levels of calcium , phosphorus , magnesium , chlorides and iron , the activities of alkaline phosphatase ( ALP ) , aspartate aminotransferase ( AST ) and alanine aminotransferase ( ALT ) , and the concentrations of total protein , albumin , cholesterol , urea , glucose , and vitamins A and E ] was studied in the blood serum of 40 anoestrous and 40 control inseminated animals in a production herd with an increased occurrence of anoestrus in gilts . - pubmed
The anoestrous gilts showed significantly lower levels of albumin ( P less than 0.01 ) and glucose ( P less than 0.01 ) and ALP activity ( P less than 0.05 ) , and significantly higher concentrations of urea ( P less than 0.01 ) , vitamin A ( P less than 0.01 ) and vitamin E ( P less than 0.05 ) and ALT activity ( P less than 0.05 ) , as compared with the inseminated controls . - pubmed
The comparison of the obtained values of the studied biochemical criteria with literary data indicated a lower concentration of magnesium and a higher ALP and ALT activities in the anoestrous and inseminated gilts in both groups under study . - pubmed
The inhibitory action of two thrombin inhibitors ( TI-189 and TI-233 ) on the contractile responses to 5 - hydroxytryptamine and prostaglandin endoperoxide analogue ( U-44069 ) in isolated vascular strips . - pubmed
Microsomes were incubated with ferrous ion ( Fe2 + ) to promote free radical production , and alpha - T levels and LP were monitored after various incubation times . - pubmed
Coinciding with alpha - T depletion was a large and rapid increase in LP . - pubmed
With outer zone microsomes , alpha - T depletion required more than 30 min , and very little LP was demonstrable during this period . - pubmed
However , once alpha - T depletion occurred , LP was rapidly initiated and reached levels similar to those obtained with inner zone preparations . - pubmed
Inhibition of LP by MnCl2 prevented the Fe ( 2 +)-induced declines in alpha - T in both zones . - pubmed
The results demonstrate the importance of alpha - T as a modulator of adrenal LP and indicate that the zonal differences in LP are largely attributable to the differences in alpha - T concentrations . - pubmed
CTLA-4 molecules may either facilitate or down - regulate the second signal to T - cells , which is provided by the interaction between the two accessory molecules CD28 and B7 . - pubmed
An unequivocal answer is given to the question of why the reported fluorescence spectra of bacteriorhodopsin ( bR568 ) have been different from one another . - pubmed
Calcium - dependent transcriptional activation through the SRE is mediated by the serum response factor ( SRF ) . - pubmed
Membrane depolarization induces phosphorylation of SRF at Ser-103 , an event shown to enhance the ability of SRF to bind the SRE . - pubmed
Ca ( 2 +)-induced SRF phosphorylation occurs via a pathway that may involve Ca2 +/ calmodulin - dependent kinases . - pubmed
The sodium , potassium ions - activated adenosine triphosphatase ( Na + , K (+)- ATPase ) activity of the erythrocyte membrane was decreased to 22 % ( P & lt ; 0.01 ) and 24 % ( P & lt ; 0.01 ) in the NaCl and NaCit groups , respectively , compared with the control groups ; this activity was decreased to 43 % in the ChoCl group ( P & lt ; 0.05 ) . - pubmed
The improved results may be due to earlier renal biopsy for precise histopathologic definition , better supportive care , and selective use of aggressive therapy , including methylprednisolone pulse therapy , intravenous cyclophosphamide , intravenous prostaglandin E1 therapy , high - dose intravenous gammaglobulin therapy , and cyclosporin A for those with high risk factors . - pubmed
The structure of a catalytically competent reaction intermediate of carboxypeptidase A ( CPA ) formed with the specific spin - label ester substrate O -[ 3 -( 2,2 ,- 5,5 - tetramethyl-1 - oxypyrrolinyl ) propen-2 - oyl ]- L - b eta - phenyllactate through application of cryoenzymological methods has been determined by electron nuclear double resonance ( ENDOR ) and molecular modeling . - pubmed
It is sensitive to the dihydropyridines nicardipine and Bay K 8644 , to calciseptine , and to an anti - peptide antibody raised against the alpha1 subunit of the L - type calcium channel , but is voltage - insensitive . - pubmed
Camptothecin and minor - groove binder hybrid molecules : synthesis , inhibition of topoisomerase I , and anticancer cytotoxicity in vitro . - pubmed
The synthesis , characterization , inhibitory activity against topoisomerase I , and biological evaluation of a series of 14 camptothecin derivatives of polypyrrolecarboxamide ( lexitropsin ) conjugates of two structural classes : ( A ) camptothecin - NHCO - lexitropsin 44 - 51 and ( B ) camptothecin - CONH - lexitropsin 38 - 43 are described . - pubmed
All 16 compounds tested , 14 conjugates plus two functionalized camptothecin controls , inhibit topoisomerase I in the concentration range 1.12 - 16.6 microM that divide into three distinct categories based on activity . - pubmed
The most active enzyme inhibitors belong to structure class A with either cationic dimethylaminium or neutral amide end groups . - pubmed
Subjects were studied at rest and during constant cycle exercise at a work rate equivalent to 40 % of their maximum oxygen consumption . - pubmed
The aim of this study was to determine the effects of desflurane on cerebral blood flow ( CBF ) velocity using transcranial Doppler ultrasonography in a setting that closely resembled usual clinical practice . - pubmed
In group 2 no significant changes in systemic haemodynamic responses were measured after desflurane administration ; however , CBF velocity increased to 73 + /- 5 cm s-1 ( 59 % increase ) . - pubmed
The results indicate that desflurane increases CBF velocity concurrently with induction of tachycardia and hypertension . - pubmed
Although sufentanil and N2O attenuate the systemic haemodynamic alterations caused by desflurane , the CBF velocity increases . - pubmed
The inhibitory efficacy of sulbactam , clavulanic acid and tazobactam over TEM-1 is inversely proportional to the amounts of enzyme produced . - pubmed
The three principal sarcoplasmic / endoplasmic reticulum Ca ++ pump inhibitors have been compared for their effects on Ca ++ fluxes across intracellular stores present in isolated skeletal muscle and brain membrane preparations . - pubmed
These observations taken together suggest that transient releases were manifest primarily by vesicles with a high ratio of ryanodine receptors to pumps ( and thus more susceptible to becoming leaky with only some pumps inhibited ) , and that Ca(++)-induced Ca ++ release amplified releases when all pumps were blocked . - pubmed
One subset of compounds consists of fadrozole analogues and was studied in a previous work , from which a ' local ' 3 - D quantitative structure - activity relationship ( QSAR ) model for the inhibition of aromatase was obtained . - pubmed
In the present paper , that model is extended to include a second subset of compounds bearing a tetralone nucleus and acting at the same enzyme site with the same mechanism as the azoles . - pubmed
In the present investigation the effects of NPY on whole cell Ba2 + currents were examined to obtain direct evidence supporting the mechanisms suggested by those studies . - pubmed
This inhibition was pertussis toxin sensitive and resulted from NPY modulation of L- and N - type Ca2 + channels . - pubmed
The inhibition of L - type channels was not seen with & lt ; 1 nM free intracellular Ca2 + or when protein kinase C ( PKC ) was inhibited by chelerythrine or PKC -( 19 - 31 ) . - pubmed
Furthermore , the effect of NPY on L - type channels was mimicked by the PKC activator phorbol 12 - myristate 13 - acetate . - pubmed
Syndecan-1 , a transmembrane heparan sulphate proteoglycan ( HSPG ) , functions as a matrix receptor on the basal surface of epithelial cells . - pubmed
Similarly , there was a significant reduction of both molecules in carcinomas compared with associated adenomas ( syndecan-1 P = 0.00003 ; E - cadherin P = 0.002 ) . - pubmed
We have previously shown that a range of nicotinamide containing ' biomimetic coenzymes ' function as active analogues of NAD + in the oxidation of alcohols by horse liver alcohol dehydrogenase ( HLADH ) , despite their apparently astonishing lack of structural similarity to the natural coenzyme . - pubmed
An HPLC - based assay suggested that two of the new analogues were coenzymically active in the oxidation of butan-1 - ol by HLADH , with one displaying a significantly higher activity than CL4 . - pubmed
Ventilator management was aimed at maintaining comparable values of end tidal carbon dioxide , Hb saturation , and arterial blood gases . - pubmed
Multitargeted antifolate ( MTA , LY231514 ) is a novel antifolate antimetabolite , with antitumor activity via inhibition of thymidylate synthase , glycinamide formyl transferase , and dihydrofolate reductase . - pubmed
Intrapleural therapy with MDP - Lys ( L18 ) , a synthetic derivative of muramyl dipeptide , against malignant pleurisy associated with lung cancer . - pubmed
N2 -[( N - acetylmuramoyl )- L - alanyl - D - isoglutaminyl ]- N6 - stearoyl - L - lysine ( MDP - Lys ( L18 ) , romurtide ) is a synthetic muramyl dipeptide derivative , and has immunomodulating activities including activation of cells of monocyte - macrophage lineage . - pubmed
We examined the effect of intrapleural instillation of MDP - Lys ( L18 ) against malignant pleurisy associated with lung cancer . - pubmed
These results suggest MDP - Lys ( L18 ) instilled by intrapleural route had a potential local immunomodulatory activity . - pubmed
Chiral monodentate phosphine ligand MOP for transition - metal - catalyzed asymmetric reactions . - pubmed
Luminal epithelial cells , glandular epithelial cells and stromal cells were isolated from cyclic , pregnant or oestrogen - induced pseudopregnant gilts on Day 12 ( Experiment 1 ) or Day 16 ( Experiment 2 ) post oestrus ( oestrus = Day 0 ) . - pubmed
Polynitroxyl albumin ( PNA ) is a compound of human serum albumin covalently labeled with nitroxides that exhibits a prolonged half - life and an enhanced antioxidant activity . - pubmed
Discordant results were distributed equally among specimens from drug - experienced and drug - naive individuals and were slightly higher for protease inhibitors than for nonnucleoside reverse transcriptase inhibitors or nucleoside reverse transcriptase inhibitors . - pubmed
Glucose released from storage in liver glycogen by phosphorylase accounts for approximately 50 % of the glucose produced after an overnight fast . - pubmed
However , the same combination reduced HLGPa activity , and the inhibition was glucose dependent . - pubmed
The positive staining for GLUT-2 observed for islets cells and all islet tumors is consistent with the notion that all pancreatic islet cells and islet cell tumors utilize glucose as a major fuel , requiring transporter - facilitated diffusion of glucose into the cells of normal organ and their tumors . - pubmed
Structurally diverse carbon - linked ( C - linked ) analogs of antifreeze glycoprotein ( AFGP ) have been prepared via linear or convergent solid phase synthesis . - pubmed
Sulfa resistance and dihydropteroate synthase mutants in recurrent Pneumocystis carinii pneumonia . - pubmed
The results suggest that DHPS mutations can be selected de novo within patients by the pressure of a sulfa or sulfone drug . - pubmed
It was recently discovered that VGLUT1 , previously characterized as an inorganic phosphate transporter ( BNPI ) with 9 - 11 predicted transmembrane spanning domains , is capable of transporting glutamate . - pubmed
The enzyme 20alpha - hydroxysteroid dehydrogenase ( 20alpha - HSD ) catalyzes the conversion of progesterone into its inactive form , 20alpha - hydroxyprogesterone . - pubmed
These results suggest that progesterone can be inactivated by 20alpha - HSD in some specific brain areas . - pubmed
Evolved alachlor was collected in ethylene glycol , recovered with C18 solid phase extraction cartridges , and analyzed by reverse - phase high - performance thin - layer chromatography with densitometry . - pubmed
Parameters of bone metabolism ( intact parathyroid hormone , serum osteocalcin and urinary deoxypyridinoline ) and BMD were assessed before and after the study period . - pubmed
Nociceptin , a 17 amino acid opioid - like peptide that has an inhibitory effect on synaptic transmission in the nervous system , is involved in learning , memory , attention , and emotion and is also implicated in the perception of pain and visual , auditory , and olfactory functions . - pubmed
In this study , we investigated the NMR solution structure of nociceptin in membrane - like environments ( trifluoroethanol and SDS micelles ) and found it to have a relatively stable helix conformation from residues 4 - 17 with functionally important N - terminal residues being folded aperidoically on top of the helix . - pubmed
In functional assays for receptor binding and calcium flux , alanine - scanning variants of nociceptin indicated that functionally important residues generally followed helix periodicity , consistent with the NMR structural model . - pubmed
CONCLUSION : ICV injection of STZ increases the expression of Abeta and promotes hyerphosphorylation of tau protein . - pubmed
Adjustment by multiple logistic regression for hypertension , triglycerides , sex , non - HDL cholesterol , BMI , smoking , and alcohol intake did not diminish the association ( OR = 1.8 , P = 0.003 ) . - pubmed
Subjects with HL TT exhibited trends toward increased triglyceride and non - HDL cholesterol levels compared with CC carriers . - pubmed
CONCLUSIONS : HL T+ genotypes might increase the risk of developing diabetic nephropathy by slowing clearance of triglyceride - rich remnant lipoproteins . - pubmed
Hydroxy - methyl - glutaryl coenzyme A ( HMG - CoA ) reductase inhibitors have pleiotropic actions that affect many systems other than lowering blood cholesterol concentrations . - pubmed
These isoprenoid moieties are required for protein prenylation and HMG - CoA reductase inhibitors impair this function in some cells . - pubmed
Other mechanisms have also been proposed including an increase in the free fraction of cyclosporin and a reduction in the time that low density lipoprotein ( LDL ) spends in blood . - pubmed
Drug interactions between cyclosporin and HMG - CoA reductase inhibitors are a limiting factor to their use . - pubmed
Pravastatin appears to be the best HMG - CoA reductase inhibitor for organ transplant recipients because of its lesser potential to interact with cyclosporin and hence cause myositis , which may thus allow higher doses to be used . - pubmed
BACKGROUND : DE-310 is composed of the topoisomerase - I - inhibitor DX-8951 ( exatecan ) and a biodegradable macromolecular carrier , which are covalently linked by a peptidyl spacer . - pubmed
For quantitation of CoQ10 in food samples , 2 liquid chromatography ( LC ) methods with UV and mass spectrometry ( MS ) detections were developed . - pubmed
LC / MS analysis was performed on a LUNA C18 3 microm , 150 x 4.6 mm column , using mobile phase consisting of ethanol - dioxane - acetic acid ( 9 + 1 + 0.01 , v / v / v ) , flow rate was 0.6 mL / min , and the retention time of CoQ10 was 4.1 + /- 0.1 min . - pubmed
An extracellular agarase was purified by the procedure of ammonium sulfate precipitation , gel filtration on Sephacryl S-100HR , and ion - exchange chromatography on diethylaminoethyl - Sepharose . - pubmed
The enzyme hydrolyzed the beta-1,4 - glycosidic linkages of agar , yielding neoagarotetraose and neoagarohexaose as the main products . - pubmed
The optimum reaction temperature of the agarase was 35 degrees C , with a narrow range from 30 to 45 degrees C. The enzyme activity reached the maximum at pH 7.0 and in the presence of 2 % NaCl . - pubmed
There were no significant differences in total cholesterol , LDL - cholesterol and apoB between the TRANS- and the PALM - diet . - pubmed
HDL - cholesterol and apoA - I were significantly higher on the PALM - diet compared to the TRANS - diet while the ratio of LDL- to HDL - cholesterol was lower , although not significantly ( P = 0.077 ) on the PALM - diet . - pubmed
Total cholesterol , LDL - cholesterol and apoB were significantly lower on the PUFA - diet compared to the two other diets . - pubmed
Triglycerides and Lp ( a ) were not different among the three diets . - pubmed
t - PA activity was also decreased on the PUFA - diet compared to PALM - diet although not significantly ( P=0.07 ) . - pubmed
Two - dimensional fluorescence correlation spectroscopy IV : resolution of fluorescence of tryptophan residues in alcohol dehydrogenase and lysozyme . - pubmed
Generalized two - dimensional ( 2D ) fluorescence correlation spectroscopy has been used to resolve the fluorescence spectra of two tryptophan ( Trp ) residues in alcohol dehydrogenase and lysozyme . - pubmed
In each protein , one Trp residue is buried in a hydrophobic domain of the protein matrix and the other Trp residue is located at a hydrophilic domain close to the protein - water interface . - pubmed
Fluorescence spectra of the two Trp residues in alcohol dehydrogenase , which are heavily overlapped in conventional one - dimensional spectra , have been successfully resolved by the 2D correlation technique . - pubmed
In contrast , the fluorescence spectra of the two Trp residues could not be resolved after the alcohol dehydrogenase was denatured with guanidine hydrochloride . - pubmed
Fluorescence spectra of the Trp residues in lysozyme could not be resolved by the 2D correlation technique . - pubmed
The differences between the two proteins are attributed to the fact that the Trp residue in the hydrophobic site of lysozyme is not sufficiently protected from the quenching . - pubmed
Temporal expression of IL-1beta protein and mRNA in the brain after systemic LPS injection is affected by age and estrogen . - pubmed
Using the latter approach , we have shown that estrogen suppresses inflammatory cytokine expression in lesioned ovariectomized young adult females but not reproductive senescent animals . - pubmed
However , in cultured microglia derived from either young or senescent animals , estrogen fails to suppress LPS - induced cytokine expression . - pubmed
However , estrogen treatment attenuated IL-1beta expression in the olfactory bulb but not in the pituitary gland . - pubmed
In senescent animals , estrogen completely suppressed IL-1beta expression in the bulb and the pituitary gland , while placebo - replaced animals responded normally . - pubmed
Age and hormone effects on pituitary cytokines were also mirrored in plasma corticosterone ( CORT ) levels , such that estrogen treatment to senescent females attenuated LPS - induced CORT . - pubmed
1 - Cinnamoyl-3,11 - dihydroxymeliacarpin is a natural bioactive compound with antiviral and nuclear factor - kappaB modulating properties . - pubmed
CDM blocks VSV entry and the intracellular transport of VSV - G protein , confining it to the Golgi apparatus , by pre- or post - treatment , respectively . - pubmed
In conclusion , CDM is a pleiotropic agent that not only inhibits the multiplication of DNA and RNA viruses by the same mechanism of action but also modulates the NF - kappaB signaling pathway . - pubmed
Elevations in mitochondrial LPO along with decrements in enzymic antioxidants ( superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione peroxidase ( GPx ) , glutathione reductase ( GR ) and glutathione - S - transferase ( GST ) ) , non - enzymic antioxidants ( reduced glutathione ( GSH ) , vitamin C , vitamin E and vitamin A ) , citric acid cycle enzymes ( isocitrate dehydrogenase ( ICDH ) , alpha - ketoglutarate dehydrogenase ( alpha - KDH ) , succinate dehydrogenase ( SDH ) and malate dehydrogenase ( MDH ) ) , and respiratory chain enzymes ( NADH dehydrogenase and Cytochrome c oxidase ) were observed in B ( a ) P ( 50mg / kg body weight ) administered animals . - pubmed
CAP ( 10mg / kg body weight ) pretreatment decreased lung mitochondrial LPO and augmented the activities of enzymic , non - enzymic antioxidants , citric acid cycle enzymes and respiratory chain enzymes to near normalcy revealing its chemoprotective function during B ( a ) P induced lung cancer . - pubmed
There is no data on the influence of PTH fractions ( CAP and CIP ) on bone turnover and density in hyperprolactinemia . - pubmed
In contrast , PTH treatment had no effect on periosteal bone formation and minimal effects on DABM - induced osteoinduction . - pubmed
There was no interaction between PTH treatment and alcohol consumption for any of the endpoints evaluated . - pubmed
Furthermore , alcohol inhibits osteoinduction and reduces periosteal and cancellous bone formation , irrespective of therapeutic PTH administration . - pubmed
When sucrose was hydrolyzed in an aqueous suspension of the copoly(MA , St)-coated capsules into which invertase was loaded , the hydrolytic reaction was initiated at pH 5 . - pubmed
Systemic administration of tumour necrosis factor ( TNF )- alpha induces the release of norepinephrine in the paraventricular nucleus ( PVN ) of hypothalamus and an increase in expression of corticotrophin - releasing factor ( CRF ) and CRF type 1 receptors . - pubmed
We explored the hypothesis that CRF and norepinephrine in PVN mediate the cardiovascular and sympathetic responses to acute systemic administration of TNF - alpha . - pubmed
Prazosin blocked the TNF - alpha - induced increase in renal sympathetic nerve activity ( RSNA ) , whereas alpha - helical CRF substantially reduced the RSNA response . - pubmed
Conversely , CRF and the alpha 1 - adrenergic agonist phenylephrine , administered ICV , both elicited increases in PVN neuronal activity , RSNA , arterial pressure and heart rate . - pubmed
Microinjection of CRF and phenylephrine directly into PVN evoked smaller responses . - pubmed
These results are consistent with the hypothesis that norepinephrine and CRF in the PVN mediate the cardiovascular and sympathetic responses to acute systemic administration of TNF - alpha . - pubmed
Lipid profile changed thus : total cholesterol 4.97 ... 5.01mmol / l ( p = 0.7 ) , LDL cholesterol 2.83 ... 2.82 mmol / l mmol / l ( p = 0.9 ) , HDL cholesterol 1.13 ... 1.27 mmol / l ( p = 0.003 ) , triglycerides 1.98 ... 1.91 mmol / l ( p = 0.01 ) . - pubmed
A novel cellulose - based polyelectrolyte ( AM - C ) containing acylamino ( DS=0.625 ) and carboxyl ( DS=0.148 ) groups was homogeneously synthesized from cellulose with acrylamide in NaOH / urea aqueous solutions . - pubmed
The results indicated that AM - C could form large aggregates spontaneously in water with or without the addition of salts by the strong hydrogen bonds and electrostatic interaction between acylamino and carboxyl groups . - pubmed
Ammonium can be assimilated onto nitrogenous organic compounds by the concerted action of two enzymes that compose the glutamine synthetase ( GS , EC 6.3.1.2 ) - glutamate synthase ( Fd - GOGAT , EC 1.4.7.1 ; NADH - GOGAT , EC 1.4.1.14 ) cycle . - pubmed
Ammonium may also be directly incorporated into glutamate by the glutamate dehydrogenase ( GDH , EC 1.4.1.2 ) aminating reaction . - pubmed
However , as GDH reversibly deaminates glutamate , its physiological role in vivo remains controversial . - pubmed
Regarding GS gene - specific mRNA accumulation , an organ - dependent response was also observed that contributes for the detected alteration in the ammonium assimilatory metabolism . - pubmed
The possible contribution of GDH for ammonia assimilation was also investigated . - pubmed
Adjusted models indicated a modest risk among AZA patients for graft loss ( AHR = 1.14 , 95 % CI 1.07 - 1.20 ) ; however , this was not apparent among AZA patients also treated with tacrolimus ( AHR = 0.97 , 95 % CI 0.85 - 1.11 ] . - pubmed
Therefore , it was intuitive to obtain the sequence of the antitumor activity of four anticancer drugs without data processing as follows : mitoxantrone ( MIT ) & gt ; pirarubicin ( PIR ) & gt ; daunorubicin ( DAU ) & gt ; doxorubicin ( DOX ) by RLS screening spectra . - pubmed
Moreover , the apparent equilibrium constant ( K ) was 1.23 x 10(4 ) , 2.22 x 10(4 ) , 4.66 x 10(4 ) L / mol for DOX , DAU , and PIR , respectively . - pubmed
The inhibitory concentration 50 ( IC50 ) was 0.148 , 0.102 , 0.025 , 0.013 micromol / L for DOX , DAU , PIR , MIT , respectively . - pubmed
Therefore , the antitumor effect of four drugs was as follows : MIT & gt ; PIR & gt ; DAU & gt ; DOX , which was in good agreement with the result obtained from RLS screening assays . - pubmed
1 and 2 are isomorphous , they possess a three - dimensional architecture constructed from Ln ( 3 + ) ions bridged by oxalate anions and two types of 1H-6 - Opy-3 - CO ( 2 )(- ) bridges , showing a three - nodal ( 4,5)-connected topology ( 3.4(2).5(2).6(3).7.8)(2)(3.5(3).6(2))(2)(3(2).6.7(2).8 ) or a simplified uninodal 6 - connected topology ( 3(3).4(6).5(5).6 ) , both topologies are completely new ; while only one type of 1H-6 - Opy-3 - CO ( 2 )(- ) bridge is used to construct the two - dimensional layer networks of 3 and 4 besides oxalate bridges , both complexes 3 and 4 are isostructural , exhibiting the honeycomb topology 6(3 ) . - pubmed
Regional Tc 99m - DTPA GFR of both affected kidney and nonaffected kidney showed no significant differences perioperatively ( 6.3 + - 1.8 to 6.1 + - 1.9 ml / min per 1.73 m2 ; p = 0.641 ; 6.6 + - 1.9 to 7.1 + - 2.0 ml / min per 1.73 m2 ; p = 0.200 ) . - pubmed
Cytochrome c nitrite reductase , NrfA , catalyzes the six - electron reduction of nitrite , NO ( 2 )(- ) , to ammonium , NH ( 4 )(+ ) , as the final enzymatic step in the dissimilatory metabolic pathway of nitrite ammonification within the biogeochemical nitrogen cycle . - pubmed
NrfA is a 55 - 65kDa protein that binds five c - type heme groups via thioether bonds to the cysteines of conserved CXXCH heme attachment motifs . - pubmed
While no intermediates of the reaction are released , NrfA is able to reduce various other nitrogen oxides such as nitric oxide ( NO ) , hydroxylamine ( H ( 2 ) NOH ) , and nitrous oxide ( N ( 2 ) O ) , but notably also sulfite , providing the only known direct link between the nitrogen and sulfur cycles . - pubmed
In many enterobacterial species , NrfA is linked to the menaquinol pool in the cytoplasmic membrane through a soluble electron carrier , NrfB , that in turn interacts with a membrane - integral quinol dehydrogenase , NrfCD . - pubmed
AIM OF THE STUDY : Reperfusion following cerebral ischemia leads to excessive production of reactive oxygen species ( ROS ) and consumption of endogenous antioxidants . - pubmed
Copper , zinc - superoxide dismutase ( SOD1 ) is one of the major defensive mechanisms by which cells counteract the deleterious effects of ROS after ischemia . - pubmed
Our recent research provides novel molecular and functional evidence that indoxyl sulfate , an anionic uremic toxin , undergoes efflux transport at the BBB via OAT3 and creatinine , a uremic guanidino compound , undergoes efflux transport at the BCSFB via OCT3 . - pubmed
EDTA - imipenem disc synergy tests , and polymerase chain reaction amplification and sequencing were performed to detect the presence of metallo - beta - lactamases ( MBLs ) . - pubmed
Na delivery and ENaC mediate flow regulation of collecting duct endothelin-1 production . - pubmed
Collecting duct ( CD ) endothelin-1 ( ET-1 ) is an important autocrine inhibitor of Na and water transport . - pubmed
CD ET-1 production is stimulated by extracellular fluid volume expansion and tubule fluid flow , suggesting a mechanism coupling CD Na delivery and ET-1 synthesis . - pubmed
Flow with 300 mosmol / l NaCl increased ET-1 mRNA to 65 % above that observed under static conditions . - pubmed
Increasing perfusate osmolarity to 450 mosmol / l with NaCl or Na acetate increased ET-1 mRNA to ~184 % compared with no flow , which was not observed when osmolarity was increased using mannitol or urea . - pubmed
Inhibition of epithelial Na channel ( ENaC ) with amiloride or benzamil abolished the flow response , suggesting involvement of ENaC in flow - regulated ET-1 synthesis . - pubmed
Since Ca ( 2 + ) enhances CD ET-1 production , the involvement of plasma membrane and mitochondrial Na / Ca ( 2 + ) exchangers ( NCX ) was assessed . - pubmed
SEA0400 and KB - R7943 , plasma membrane NCX inhibitors , did not affect the flow response . - pubmed
However , CGP37157 , a mitochondrial NCX inhibitor , abolished the response . - pubmed
In summary , the current study indicates that increased Na delivery , leading to ENaC - mediated Na entry and mitochondrial NCX activity , is involved in flow - stimulated CD ET-1 synthesis . - pubmed
Dermatological adverse effects during genotype-1 hepatitis C treatment with the protease inhibitors telaprevir and boceprevir . - pubmed
Of the three compounds , apigenin-8 - C - beta - D -( 2 "- O - alpha - L - rhamnosyl ) glucopyranoside was identified based on its UV , MS , and NMR spectra . - pubmed
PYR / RCAR receptors contribute to ozone- , reduced air humidity- , darkness- , and CO2 - induced stomatal regulation . - pubmed
Rapid stomatal closure induced by changes in the environment , such as elevation of CO2 , reduction of air humidity , darkness , and pulses of the air pollutant ozone ( O3 ) , involves the SLOW ANION CHANNEL1 ( SLAC1 ) . - pubmed
OST1 activation is controlled through abscisic acid ( ABA)-induced inhibition of type 2 protein phosphatases ( PP2C ) by PYRABACTIN RESISTANCE / REGULATORY COMPONENTS OF ABA RECEPTOR ( PYR / RCAR ) receptor proteins . - pubmed
The stomatal conductance values varied severalfold among the studied mutants , indicating that basal ABA signaling through PYR / RCAR receptors plays a fundamental role in controlling whole - plant water loss through stomata . - pubmed
PYR / RCAR - dependent inhibition of PP2Cs was clearly required for rapid stomatal regulation in response to darkness , reduced air humidity , and O3 . - pubmed
Although a rapid stomatal response to elevated CO2 was evident in all but slac1 and ost1 mutants , the bicarbonate - induced activation of S - type anion channels was reduced in the dominant active PP2C mutants abi1 - 1 and abi2 - 1 . - pubmed
Thus , we show that PYR / RCAR receptors play an important role for the whole - plant stomatal adjustments and responses to low humidity , darkness , and O3 and are involved in responses to elevated CO2 . - pubmed
Role of the central arginine R133 toward the ion selectivity of the phosphate specific channel OprP : effects of charge and solvation . - pubmed
A detailed investigation of the structure - function relationship of OprP is inevitable to decipher the anion and phosphate selectivity of this porin in particular and to broaden the present understanding of the ion selectivity of different channels . - pubmed
To this end we investigated the role of the central arginine of OprP , R133 , in terms of its effects in selectivity and ion transport properties of the pore . - pubmed
For these mutants , the change in phosphate binding specificity , ion conduction , and anion selectivity was determined and compared to previous molecular dynamic calculations and electrophysiological measurements with wild - type OprP. - pubmed
Herein , we demonstrate that ionic supramolecular bonds , introduced by infiltration of divalent Cu ( 2 + ) ions , allow efficient stabilization of the mechanical properties of self - assembled water - borne nacre - mimetics based on sustainable sodium carboxymethylcellulose ( Na(+)CMC ) and natural sodium montmorillonite nanoclay ( Na(+)MTM ) against high humidity ( 95 % RH ) . - pubmed
To illustrate the interaction between CCR5 and microglia in the CNS , we used a model of excitotoxicity , and demonstrate the intimate involvement of CCR5 in neuron injury and inflammation attendant to kainic acid ( KA)-induced neurotoxicity . - pubmed
Acquired factor VIII inhibitor and positive lupus anticoagulant testing on silica clotting time . - pubmed
AF can be terminated in 3 modes : ( 1 ) directly into sinus rhythm ( SR ) ; ( 2 ) evolving into regular atrial tachycardia ( AT ) and subsequently into SR ; and ( 3 ) after direct current ( DC ) cardioversion if AF persists . - pubmed
CONCLUSIONS : The use of this amount of pentoxifylline did not modify the serum levels of hepcidin in this population . - pubmed
Epigallocatechin-3 - gallate ( EGCG ) exerts anti - inflammatory properties on immune cells and binds to CD4 molecules . - pubmed
CD4 blockade did not suppress the increase in IFN - gamma - induced STAT1 activation in CD4(+ ) T cells by EGCG . - pubmed
Interestingly , EGCG promoted apoptosis of CD4(+ ) T cells treated with IFN - gamma . - pubmed
Moreover , EGCG alleviates CD4(+ ) CD45RB(hi ) CD25(- ) T cell transfer induced colitis with less accumulation of CD4(+ ) T cells in the colon . - pubmed
Our findings suggest a novel role of EGCG in regulating IFN - gamma signaling and controlling inflammation . - pubmed
The Effects of a GLP-1 Analog on Glucose Homeostasis in Type 2 Diabetes Mellitus Quantified by an Integrated Glucose Insulin Model . - pubmed
The effect of liraglutide was incorporated in the model by including a stimulatory effect on insulin secretion . - pubmed
As other GLP-1 receptor agonists have similar modes of action , it is believed that the model can also be used to describe the effect of other receptor agonists on glucose homeostasis . - pubmed
An aim of this study is to investigate whether the combination of US - targeted microbubble destruction ( UTMD ) and polyethylenimine ( PEI ) ( UTMD / PEI ) can mediate even greater gene transfection efficiency than UTMD alone and to optimize ultrasonic irradiation parameters . - pubmed
UTMD / PEI , combined with albumin - coated microbubbles , warrants further investigation for therapeutic gene delivery . - pubmed
parareesei Tparo7 gene , encoding a chorismate mutase ( CM ) , a shikimate pathway branch point leading to the production of aromatic amino acids , which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites . - pubmed
In addition , the plants turned yellowish and were defective in jasmonic acid- and ethylene - regulated signaling pathways which was seen by expression analysis of lipoxygenase 1 ( LOX1 ) , ethylene - insensitive protein 2 ( EIN2 ) and pathogenesis - related protein 1 ( PR-1 ) genes . - pubmed
These brackets along with stainless steel ( SS ) or nickel - titanium ( NiTi ) orthodontic wires ( 0.016 " , round ) were immersed in Oral - B mouthwash containing 0.05 % sodium fluoride for 28 days . - pubmed
The electric potential ( EP ) difference of each bracket - wire couple was measured with a Saturated Calomel Reference Electrode ( Ag / AgCl saturated with KCl ) via a voltmeter . - pubmed
The copper ions released from specimens with NiTi wire were greater than those of samples containing SS wire . - pubmed
Corrosion rate of brackets coupled with NiTi wires was higher than that of brackets coupled with SS wires . - pubmed
To evaluate whether ZMP accumulated during MTX therapy , we measured the concentration of ZMP in erythrocytes and the concentration of its dephosphorylated derivative 5 - aminoimidazole-4 - carboxamide-1 - beta - d - ribofuranoside ( AICAR ) in urine using liquid chromatography mass spectrometry ( LC - MS / MS ) . - pubmed
To assess glucose homeostasis , we determined the concentration of glycated haemoglobin ( HbA1c ) and homeostasis model assessment of insulin resistance [ HOMA - IR : fasting glucose ( mmol / L ) x fasting insulin ( muU / mL)/22.5 ] . - pubmed
Herein we report a novel tumor vascular - targeting multidrug delivery system using mesoporous silica nanoparticles as carrier to co - load an antiangiogenic agent ( combretastatin A4 ) and a chemotherapeutic drug ( doxorubicin ) and conjugate with targeting molecules ( iRGD peptide ) for combined anti - angiogenesis and chemotherapy . - pubmed
Wheel running increased the percentage of BrdU - labeled cells expressing DCX in the SVZ , whereas CORT treatment decreased this percentage . - pubmed
In addition , CORT can suppress the cell differentiation within the SVZ , and this was alleviated by wheel running as indicated by the double labeling of BrdU and DCX . - pubmed
Serum FGF23 , 1 , 25 - dihydroxy vitamin D3 and parathyroid hormone were measured by enzyme - linked immunosorbent assay . - pubmed
FGF23 remained significantly associated with serum creatinine ( P = 0.046 ) and estimated glomerular filtration rate ( P = 0.034 ) after full adjustment . - pubmed
Cytoplasmic and nuclear extracts were isolated from fresh cerebrum and cerebellum and used to determine thioredoxin ( Trx ) and glutathione ( GSH ) levels , as well as thioredoxin reductase ( TrxR ) and glutathione peroxidase ( GPx ) activities . - pubmed
MeHg - induced antioxidant response was not uniform for all the analyzed antioxidant molecules , and sexual dimorphism in response to MeHg treatment was evident for TrxR , Trx and GPx . - pubmed
The study demonstrates for the first time sex - and structure - specific changes in the response of the thioredoxin system to MeHg neurotoxicity and suggests that these differences in antioxidant responses might impart differential susceptibility to developmental MeHg exposure . - pubmed
One such efficacious therapy is the anti - CD38 monoclonal antibody , daratumumab ( Dara ) . - pubmed
A free software for the calculation of T2 * values for iron overload assessment . - pubmed
Background Iron overload assessment with magnetic resonance imaging ( MRI ) using T2 * has become a key diagnostic method in the management of many diseases . - pubmed
This mutation substitutes a highly conserved glutamic to arginine at the position of the 47th amino acid which was shown to be located on the flank of the pleated sheet domain in PATE1 protein by the 3D model given by the Protein Model Portal ( PMP ) . - pubmed
Independent activation of ion conduction pores in the double - barreled calcium - activated chloride channel TMEM16A. - pubmed
The TMEM16 proteins constitute a family of membrane proteins with unusual functional breadth , including lipid scramblases and Cl- channels . - pubmed
Members of both these branches are activated by Ca2 + , acting from the intracellular side , and probably share a common architecture , which was defined in the recent structure of the lipid scramblase nhTMEM16 . - pubmed
The structural features of subunits and the arrangement of Ca2 + -binding sites in nhTMEM16 suggest that the dimeric protein harbors two locations for catalysis that are independent with respect to both activation and lipid conduction . - pubmed
Here , we ask whether a similar independence is observed in the Ca2 + -activated Cl- channel TMEM16A. - pubmed
Our results strongly suggest that TMEM16A contains two ion conduction pores that are independently activated by Ca2 + binding to sites that are embedded within the transmembrane part of each subunit . - pubmed
The established correlation between the extinction horizon and lowest CAMP basalts allows this sedimentary Hg excursion to be stratigraphically tied to a specific flood basalt unit , strengthening the case for volcanic Hg as the driver of sedimentary Hg / TOC spikes . - pubmed
Additional Hg / TOC peaks are also documented between the extinction horizon and the Triassic - Jurassic boundary ( separated by ~200 ky ) , supporting pulsatory intensity of CAMP volcanism across the entire province and providing direct evidence for episodic volatile release during the initial stages of CAMP emplacement . - pubmed
The antioxidant action of MRPs was also evaluated using a peroxide - scavenging test based on crocin bleaching . - pubmed
Our PEGylated MMNPs are highly stable in both phosphate - buffered saline ( PBS ) and in cell culture media and exhibit no cytotoxicity up to at least 100 mug mL-1 concentrations . - pubmed
Although liver enzymes , bilirubin and albumin demonstrated a circadian pattern , it was not associated with fatigue . - pubmed
Together with the light subunit LAT1 , CD98hc constitutes a heterodimeric transmembrane amino acid transporter . - pubmed
RESULTS : High expression levels of CD98hc resulted in an increase in mTOR pathway activation , amino acid metabolism , and DNA repair as well as downregulation of oxidative stress and autophagy . - pubmed
Transcription of the desiccation - related genes proV , STM1494 , kdpA , and otsB in response to paenibacterin treatment was investigated using reverse transcription - quantitative PCR . - pubmed
Furthermore , DEHP induced dysregulation of oxidative stress markers by increasing malondialdehyde ( MDA ) content and decreasing superoxide dismutase ( SOD ) , glutathione peroxidase ( GPx ) , and catalase ( CAT ) activities . - pubmed
Low concentration of DEHP ( & lt;=250 mg / kg ) exposure suppressed nuclear factor - E2 - related factor 2 ( Nrf2 ) signaling pathway , while high concentration of DEHP ( & gt;=500 mg / kg ) exposure activated Nrf2 - mediated defense response . - pubmed
DEHP induced splenic oxidative stress via interfering Nrf2 signal pathway and altering the transcription of its downstream genes . - pubmed
Individual free fatty acids have unique associations with inflammatory biomarkers , insulin resistance and insulin secretion in healthy and gestational diabetic pregnant women . - pubmed
Objective : We investigated the relationships of maternal circulating individual free fatty acids ( FFA ) with insulin resistance , insulin secretion and inflammatory biomarkers during mid - pregnancy . - pubmed
For example , women who had elevated DGLA ( highest quartile ) were twice as ( adjusted OR 2.06 , 95 % CI 1.42 to 2.98 ) likely to have higher interleukin ( IL )- 8 ( p<0.0001 ) levels . - pubmed
Conversely , women with high palmitoleic , oleic , and linolenic acid levels had reduced odds ( & gt;=2 - fold , p<0.01 to p<0.001 ) for having higher IL-8 , IL-6 or tumor necrosis factor - alpha levels . - pubmed
Conclusion : Our results suggest that maternal individual FFAs uniquely affect insulin resistance and secretion . - pubmed
CeO2NPs relieve radiofrequency radiation , improve testosterone synthesis , and clock gene expression in Leydig cells by enhancing antioxidation . - pubmed
The cells were collected to determine the antioxidant indices ( catalase [ CAT ] , malondialdehyde [ MDA ] , and total antioxidant capacity [ T - AOC ] ) , and the mRNA expression of the testosterone synthase genes ( Star , Cyp11a1 , and Hsd-3beta ) and clock genes ( Clock , Bmal1 , and Roralpha ) . - pubmed
Cells exposed to RF alone showed reduced levels of testosterone , T - AOC , and CAT activities , increased MDA content , and the downregulated genes expression of Star , Cyp11a1 , Hsd-3beta , Clock , Bmal1 , and Roralpha . - pubmed
Pretreatment of the cells with 128 mug / mL CeO2NPs for 24 hrs followed by RF exposure significantly increased testosterone synthesis , upregulated the expression of the testosterone synthase and clock genes , and increased the resistance to oxidative damage in Leydig cells compared with those in cells exposed to RF alone . - pubmed
Conclusion : Exposure to 1,800 MHz RF had adverse effects on testosterone synthesis , antioxidant levels , and clock gene expression in primary Leydig cells . - pubmed
Pretreatment with CeO2NPs prevented the adverse effects on testosterone synthesis induced by RF exposure by regulating their antioxidant capacity and clock gene expression in vitro . - pubmed
Distinct roles of an ionic interaction holding an alpha - helix with catalytic Asp and a beta - strand with catalytic His in a hyperthermophilic esterase EstE1 and a mesophilic esterase rPPE . - pubmed
An ionic interaction that holds an alpha - helix and a beta - strand on which catalytic Asp and His residues are located , respectively , is conserved in a hyperthermophilic esterase EstE1 ( optimum temperature 70 C ) and a mesophilic esterase rPPE ( optimum temperature 50 C ) . - pubmed
Ala substitutions caused a 5 - 10 C decrease in the optimum temperature of both EstE1 and rPPE mutants . - pubmed
The results of this study suggest that the ionic interaction between the alpha - helix with catalytic Asp and the beta - strand with catalytic His plays an important role in the active - site conformation of EstE1 and rPPE , with larger effects on the conformational flexibility of hyperthermophilic EstE1 and the thermal stability of mesophilic rPPE . - pubmed
Genetic down - regulation of CHRM4 or pharmacologic inhibition of CHRM4 using the selective antagonist PD102807 promoted BFU - E self - renewal , whereas deletion of Chrm4 increased erythroid cell production under stress conditions in vivo . - pubmed
The effects of muscarinic receptor antagonism on promoting expansion of BFU - Es were mediated by cyclic AMP induction of the transcription factor CREB , whose targets up - regulated key regulators of BFU - E self - renewal . - pubmed
Herein , cobalt phosphides anchored or embedded in spirulina - derived porous N - doped carbon matrix ( Co 2 P - NC ) are fabricated by two - step hydrothermal treatment and carbonization method , which cleverly utilized the intrinsic carbon , nitrogen and phosphorus of biomass as the source of C , N and P respectively . - pubmed
However , the presence of nitrite ( NO2 ) , a central intermediate of the nitrogen cycle , interferes with the electrochemical determination of the EAC . - pubmed
In addition to this ionotropic function , non - ionotropic signalling can be initiated through the exclusive binding of glycine or of glutamate to the NMDAR . - pubmed
Solution structures of copper ( i / ii ) complexes were calculated using density functional theory ( DFT ) and validated by both NMR and EPR spectroscopy . - pubmed
The tert - butyl hydroperoxide ( t - BHP ) exposure aging model bone mesenchymal stem cells ( BMSCs ) were reverted by using a poly - hybrid scaffold ( PS ) , which is a carbon nanotube ( CNT ) coated polycaprolactone ( PCL ) and polylactic acid ( PLA ) scaffold , combined with insulin - like growth factor-1 ( IGF ) . - pubmed
Changes in the sensitivity of the umbilical cord blood vessels to BRS , depending on the saturation with oxygen , have been emphasised , and the role and participation of kinins in closing up umbilical cord blood vessels immediately following labour have been discussed . - pubmed
The enzyme was shown to be homogenous by three different criteria : polyacrylamide disc gel electrophoresis , SDS polyacrylamide gel electrophoresis and analytical ultracentrifugation . - pubmed
The molecular weight of the alpha - amylase was determined by three different methods : sedimentation velocity - diffusion , conventional sedimentation equilibrium and SDS polyacrylamide gel electrophoresis and was found to be 57,850 ; 50,100 and 53,200 g mole-1 , respectively ( average value 53,700 ) . - pubmed
The amino acid composition of the enzyme was determined and compared with those of alpha - amylases from various other sources . - pubmed
[ Aminoacids in the keratin of skin , hair and nails in psoriasis cases ( author 's transl ) ] . - pubmed
Morphine in doses of 2.5 - 10 mg / kg definitely inhibited SI and SII potentials evoked by CTP stimulation . - pubmed
Morphine also inhibited SII potentials evoked by ITP stimulation , while it rather enhanced SI potentials evoked by ITP stimulation . - pubmed
Pentobarbital sodium in doses of 4 - 16 mg / kg tended to inhibit SI potentials , but showed no effect or rather an enhancement on SII potentials evoked by CTP stimulation . - pubmed
Pentobarbital sodium enhanced SI and SII potentials evoked by ITP stimulation . - pubmed
In a large dose of 32 mg / kg , pentobarbital sodium inhibited SI and SII potentials evoked by ITP and CTP stimulations . - pubmed
The results suggest that SII is more closely related to the analgesia due to morphine than is SI . - pubmed
The sensitivity of the Na +/ H + exchanger to the oxidant , hydrogen peroxide ( H2O2 ) and the protection by the antioxidant GSH suggests a possible role for the Na+/H+ exchange mechanism in the formation of cataracts . - pubmed
Mutations in Met168 highly decreased the specificity constant of the enzyme for penicillin G , penicillin V and phenylacetyl-4 - aminobenzoic acid and the catalytic constant kcat for phenylacetyl-4 - aminobenzoic acid . - pubmed
A putative penicillin - binding domain was located in penicillin acylase by sequence homology with other penicillin - recognizing enzymes . - pubmed
Lys374 and His481 , the conserved amino acid residues that are essential for catalysis in these enzymes , can be changed in penicillin acylase with no changes to the kcat and phenylmethylsulphonyl fluoride reactivity , but change the Km . - pubmed
The reported results might be used to alter the substrate specificity of penicillin acylase in order to hydrolyse substrates of industrial significance other than penicillins . - pubmed
A small group of lectins was identified , all with a main binding requirement of N - acetylgalactosamine that bound to all B lymphocytes but only to activated T lymphocytes expressing the interleukin-2 ( IL-2 ) receptor ( as shown by staining with the monoclonal antibody OX39 ) . - pubmed
With the knowledge of the binding requirements of these lectins , a structure can be deduced for the carbohydrate moiety which appears on T lymphocytes when activated . - pubmed
In the present study , we determined how the arachidonic acid cascade , mainly thromboxane A2 ( TXA2 ) , is related to ET-1 and ET-3 using indomethacin ( INDO ) , which inhibits the biosynthesis of prostaglandin ( PG ) , and OKY-046 , a selective inhibitor of TXA2 synthesis . - pubmed
While INDO failed to inhibit the slight decrease in hepatic blood flow induced by ET-1 , it significantly inhibited the marked decrease in hepatic blood flow elicited by ET-3 . - pubmed
In the next series of experiments , ET-1 and ET-3 were administered after administration of 20 mg / kg of OKY-046 . - pubmed
OKY-046 showed no effects in animals treated with ET-1 , as in those pre - treated with INDO , while it significantly inhibited the decreases in hepatic blood flow induced by ET-3 . - pubmed
These findings suggest that ET-1 decreases hepatic blood flow due to its direct effects although to a lesser extent than ET-3 , while ET-3 does so due not only to its direct effects but also to TXA2 - mediated effects . - pubmed
Cycloheximide blocks glucocorticoid - induced transcription of both the -353CAT and -239CAT reporter genes ( nucleotides -447 to -12 and nucleotides -239 to -12 of hGSTYBX , respectively , ligated to a CAT reporter gene ) ; therefore , our observations support previous results suggesting that hGSTYBX induction by glucocorticoids is a secondary response . - pubmed
The treatment of mesangial IgA nephropathy with cyclophosphamide , dipyridamole and warfarin . - pubmed
Cholesterol crystals and IgE - containing immune complexes in rheumatoid pericarditis . - pubmed
Examination of the pericardial fluid revealed the presence of cholesterol crystals and IgE - containing immune complexes . - pubmed
Captopril ( 8 , 16 , 32 mg / kg / day , IP ) induced a reversal of escape deficits but did not affect significantly the motor activity , suggesting that this effect was not due to motor stimulation . - pubmed
This antidepressant - like activity was comparable to that of imipramine ( 16 , 32 mg / kg / day , IP ) . - pubmed
Naloxone ( 0.5 ; 1 mg / kg , IP ) blocked the effect of captopril ( 16 mg / kg , IP ) in this test . - pubmed
Pyridostigmine partially restores the GH responsiveness to GHRH in normal aging . - pubmed
The GH response to pyridostigmine alone was also examined . - pubmed
Group I cases were given anti - schistosomal drugs ( oxamniquine and praziquantel ) , group II cases were given anti - schistosomal drugs plus prednisolone , and group III cases were given anti - schistosomal drugs plus cyclosporine . - pubmed
Direct observation of the enzyme - intermediate complex of 5 - enolpyruvylshikimate-3 - phosphate synthase by 13C NMR spectroscopy . - pubmed
The interaction of 5 - enolpyruvylshikimate-3 - phosphate ( EPSP ) synthase , 4,5 - dideoxyshikimate 3 - phosphate ( ddS3P ) , and [ 2 - 13C ]-and [ 3 - 13C ] phosphoenolpyruvate ( PEP ) has been examined by 13C NMR spectroscopy . - pubmed
Although no resonances due to a dead - end intermediate complex could be detected , an enzyme active site specific formation of pyruvate was observed . - pubmed
The interaction of EPSP synthase with shikimate 3 - phosphate ( S3P ) and [ 2 - 13C ]- or [ 3 - 13C ] PEP has been examined by 13C NMR spectroscopy . - pubmed
The resonance at 164.8 ppm has been assigned to enzyme - bound EPSP . - pubmed
The resonance at 88.9 ppm has been assigned to enzyme - bound EPSP . - pubmed
Neither mesaton nor isadrine altered the MP after blocking of the Na +/ K + pump . - pubmed
Catecholamines seem to exert a direct alpha - adrenomimetic and an indirect beta - adrenomimetic reversible blocking effect upon the activity of the Na +/ K +- ionic pump . - pubmed
The DNA stain Hoechst 33258 binds preferentially to AT base pairs . - pubmed
We investigated the bioactivities of the recombinant DNA - derived methionyl 20 K hGH ( 20K - Met - hGH ) and methionyl hGH ( 22K - Met - hGH ) . - pubmed
The receptors for insulin and insulin - like growth factor I ( IGF - I ) are closely related molecules , with an extracellular binding domain and an intracellular tyrosine kinase domain . - pubmed
Stimulation of both [ 3H ] glucose incorporation into glycogen and alpha -[ 3H ] aminoisobutyric acid uptake by insulin was half - maximal at concentrations of 1 - 5 nmol / L. These effects were blocked by the insulin receptor monoclonal antibody MA-10 , but not by the IGF - I receptor antibody alpha IR-3 . - pubmed
Stimulation of both functions by IGF - I was half - maximal at concentrations of 1 - 5 nmol / L , and these effects were inhibited by alpha IR-3 , but not by MA-10 . - pubmed
The first complex leaves neither a DNase footprint nor is the DNA protected from methylation by dimethyl sulfate . - pubmed
Vanadate , lanthanum , N - ethylmaleimide and calmodulin inhibitor R24571 inhibited this pump activity . - pubmed
Antiinflammatory actions of cyclooxygenase inhibitors may be related to inhibition of the synthesis and release of prostaglandins and thromboxane or to nonspecific actions of particular drugs . - pubmed
The role of cyclooxygenase products of arachidonic acid was studied in two leukocyte functions , free radical release and aggregation , after complement activation . - pubmed
In the presence of a protein kinase inhibitor , neither PMA nor any of the cytokines used are able to induce TNF - alpha mRNA accumulation , indicating that protein kinases may be crucially involved in signal transduction leading to activation of the TNF - alpha gene . - pubmed
This suggests that molybdate does not prevent receptor transformation , but rather inhibits disaggregation of the 8S oligomer into the 4S monomer . - pubmed
While IFN - beta mRNA rapidly decays after reaching a maximum 2 - 4 h after induction , this 12S RNA is stably maintained in the fibroblast cell for more than 16 h. Contrary to IFN - beta mRNA , the level of the 12S RNA is not further elevated by superinduction conditions ( cycloheximide treatment ) during poly(rI).poly(rC ) induction . - pubmed
Interactions of pig brain cytosolic sialidase with gangliosides . - pubmed
Reversible inactivation of cytosolic sialidase requires the presence of homogeneous micelles of GT1b or of mixed micelles ( for instance Triton X-100 and GT1b ) with a high GT1b content . - pubmed
Triton X-100 / ganglioside mixed micelles with a molar ratio above 50 , as well as small unilamellar vesicles of egg yolk lecithin and GT1b ( 7 - 15 mol% ) , did not inactivate the enzyme at all ; on the contrary these forms of ganglioside dispersion behaved as excellent substrates for the enzyme . - pubmed
It is to be concluded that under in vitro conditions the ability of ganglioside to interact with cytosolic sialidase , giving rise to catalytically inactive complexes or to Michaelis - Menten enzyme - substrate complexes , depends on the supramolecular organization of the ganglioside molecules . - pubmed
Short term effects of insulin on the exocrine pancreas in vitro , using isolated pancreatic acini , like stimulation of glucose- and aminoacid uptake , stimulation of proteinsynthesis , phosphorylation of certain proteins and stimulation of CCK induced amylase release are discussed . - pubmed
beta - Endorphin ( 2 microgram / kg IP ) has no effect on acquisition , but , when given prior to test sessions , facilitates retrieval of the two tasks . - pubmed
In addition to these effects , the pretraining administration of beta - endorphin disrupts , and that of naloxone facilitates retention of the two tasks . - pubmed
[ Pathogenetic aspects of the effect of thyroid hormones on glucose homeostasis : role of the insulin - producing apparatus of the pancreas ] . - pubmed
Maximum stimulation was dependent on the concentration of NAD + used in the assay ; an increase in Km value of NAD + was observed to parallel the increase in maximal velocity with increasing Mg2 + concentration , indicating that alterations in the catalytic properties of the E2 isozyme occur in the presence of Mg2 + . - pubmed
The presteady state burst of NADH product was observed to decrease in the presence of Mg2 + , suggesting that the rate - limiting step of the dehydrogenase reaction is altered by Mg2 + . - pubmed
No evidence for Mg2 + -induced alterations in the molecular weight properties of the E2 isozyme was observed using gel filtration column chromatography and fluorescence polarization techniques . - pubmed
Analysis of the incorporated sugars after hydrolysis of the glycoprotein showed that glucosamine and mannose were utilized in biosynthesis of the carbohydrate moiety directly whereas galactose was converted possibly to other intermediates before being incorporated into the antigen . - pubmed
Tunicamycin completely prevented the incorporation of the radiolabeled sugars into the surface glycoprotein . - pubmed
Ovarian concentrations of total CoA , free carnitine and acetylcarnitine increased 3 - fold on gonadotropin stimulation , whereas there was no marked change in total CoA and acid - soluble carnitine concentrations in the other organs . - pubmed
The prepubertal pattern of gonadotropin release can , in part , be explained on the basis of the development of pituitary GnRH sensitivity , which occurs independently of photoperiod . - pubmed
Compactin ( ML-236B ) and the related compound , mevinolin , are competitive inhibitors of 3 - hydroxy-3 - methylglutaryl coenzyme A reductase ( HMG CoA reductase ) , the rate - controlling enzyme in cholesterol synthesis . - pubmed
By blocking mevalonate synthesis , mevinolin activates this regulatory mechanism , and this in turn causes an increase in hepatic HMG CoA reductase . - pubmed
The ability to suppress the elevated HMG CoA reductase with mevalonate may prove useful in potentiating the effectiveness of mevinolin as a hypocholesterolemic agent . - pubmed
N - CAM has an unusual carbohydrate content and structure , is polydisperse in solution , and is associated with proteolytic activity leading to its spontaneous cleavage . - pubmed
Histochemical methods demonstrated the recovery of the glycogen content , the activity of succinate dehydrogenase and alpha - glycerophosphate dehydrogenase as well as increased fluorescence of catecholaminergic nerves in short - term ischemized myocardium ( experimental angina pectoris ) after stimulation of the carotid sinus nerve . - pubmed
The role of a phosphatidylcholine - specific phospholipase C in the production of diacylglycerol for nitric oxide synthesis in macrophages activated by IFN - gamma and LPS . - pubmed
Through analysis of diacylglycerol ( DAG ) synthesis and choline metabolism in activated macrophages , direct evidence is provided that NO synthesis involves the activation of an unusual phosphatidylcholine - specific phospholipase C ( PC - PLC ) and not a phosphatidylinositol - specific phospholipase C ( PI - PLC ) or phospholipase D ( PLD ) . - pubmed
Design , synthesis and characterization of photoreactive benzophenone - containing analogs of parathyroid hormone . - pubmed
Parathyroid hormone ( PTH ) regulates calcium and phosphate metabolism through a G - protein - coupled receptor which is shared with PTH - related protein ( PTHrP ) . - pubmed
Our approach to studying the nature of the bimolecular interface between hormone and receptor is to use a series of specially designed photoreactive benzophenone- ( BP- ) containing PTH analogs in " photoaffinity scanning " of the PTH / PTHrP receptor . - pubmed
In an H2O2 - driven assay , in which H2O2 replaced two of the protein components , NADH and O2 used in the standard assay , the proteolysed hydroxylase retained full activity for ethane , propane and propene , but had a 2 - 3 - fold increase with methane as substrate . - pubmed
Using polyoxyethylene bis ( imidazolyl carbonyl ) ( M(r ) 3350 ) as the crosslinking agent , increased thermostability of the hydroxylase was observed . - pubmed
Activated methoxypolyethylene glycol ( M(r ) 5000 ) was used to modify the hydroxylase which was now soluble in organic solvents as well as water and could be activated by H2O2 . - pubmed
The glycol - modified hydroxylase functioned well in organic solvents in the catalysis of propene oxidation . - pubmed
The non - reducing ends of this unit are the sites of mycolic acid attachment and , as they also appear in lipoarabinomannan ( LAM ) , the point of mannose capping in some mycobacteria . - pubmed
Upon application of degenerated oligonucleotides and the PCR - technology a number of putative odorant receptors have been cloned and sequenced . - pubmed
The addition of KCl ( from 0.05 M to 0.5 M ) to an acidic solution of monomeric creatine kinase ( pH 1.6 ) resulted in a highly cooperative transition from the partially unfolded conformation ( UA ) to the more compact conformation ( A ) with the properties of a molten globule , as probed by CD spectra and by fluorescence . - pubmed
To further define the factors required for late and very late promoter expression , we first determined that the eighteen lefs were also required for expression from two other major baculovirus promoters : the late basic 6.9 - kDa protein gene , p6.9 , and the very late 10 - kDa protein gene , p10 . - pubmed
Within 12 - 24 h after elevation of Ca2 + , a selective loss of the alpha 6 beta 4 integrin complex is associated with the induction of the spinous cell marker keratin 1 . - pubmed
Whereas early keratinocyte maturation is linked to the selective loss of the alpha 6 beta 4 complex , loss of both beta 1 and beta 4 integrin mRNA and protein occurs as cells proceed to later stages in the differentiation program as induced by 0.5 mM Ca2 + or suspension culture . - pubmed
Adjuvant hepatic arterial IL-2 and MMC , 5 - FU after curative resection of colorectal liver metastases . - pubmed
The regimen consisted of weekly interleukin-2 ( 1.4 - 2x 10(6 ) units ) , 5 - fluorouracil ( 250 mg ) by 2 - hour infusion and bolus mitomycin C ( 4 mg ) for 6 months . - pubmed
The efficient concentration ranges of other vitamin D3 metabolites suggest a mediation through the VD nuclear receptor ( VDR ) . - pubmed
Antiinflammatory 4,5 - diarylimidazoles as selective cyclooxygenase inhibitors . - pubmed
Variables evaluated were : disease duration , clinical pattern , disease activity ( SLEDAI ) , cumulative damage index ( SLICC / ACR ) , current and cumulative prednisone dose , duration of steroid treatment , and use of immunosuppressive agents . - pubmed
Tanshione II - A induced a dose- and time - dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly ( ADP - ribose ) polymerase in both cell lines . - pubmed
The causative biochemical defect has been identified as a deficiency of the enzyme fatty aldehyde dehydrogenase , a component of fatty alcohol : NAD + oxidoreductase . - pubmed
Within the lumen , Tsp removes the carboxy - terminal tail of the precursor of the PSII D1 protein . - pubmed
The ATP - dependent metalloprotease FtsH is bound to the thylakoid membrane , facing the stroma . - pubmed
Since ATP - dependent bacterial proteases and their mitochondrial homologues are also involved in the regulation of gene expression , via their determining the levels of key regulatory proteins , chloroplast proteases are expected to play a similar role . - pubmed
Interpreting trends in the binding of cyclic ureas to HIV-1 protease . - pubmed
Finally we verified that the ex vivo sst2 gene expression in tumor samples was positively related ( P & lt ; 0.01 ) to the in vivo semiquantitative determination of sst2 protein , assessed by 111In - pentetreotide imaging . - pubmed
We have recently shown that IL-3R occupancy activates a phosphatidylcholine - specific phospholipase C , and the sustained diacylglycerol accumulation subsequently activates protein kinase C ( PKC ) . - pubmed
The report of a PKC activatable cAMP response element ( CRE ) in the bcl-2 promoter and a role for PKC in bcl-2 expression in B cells led us to the hypothesis that PKC phosphorylation activates transcription factor CREB after IL-3R engagement . - pubmed
IL-4 , which is biologically active in these cells but does not use the beta common subunit , did not phosphorylate CREB on Ser(133 ) . - pubmed
OBJECTIVES : This study clarified the exposure - response relationships for the organic acid anhydrides ( OAA ) hexahydrophthalic ( HHPA ) and methylhexahydrophthalic ( MHHPA ) anhydrides and the development of specific immunoglobulin ( IG ) E and G antibodies and work - related symptoms . - pubmed
CONCLUSIONS : In spite of the very low OAA levels in the air and metabolites in the urine , there were high and exposure - related risks of specific IgE and IgG sensitization and of work - related symptoms for the eyes , nose ( especially bleeding ) , and lower airways . - pubmed
Many neurones co - expressed the leptin and cholecystokinin type A receptors , or leptin receptor and cocaine- and amphetamine - related transcript . - pubmed
We conclude that vagal afferent neurones that express the cholecystokinin type A receptor and cocaine- and amphetamine - related transcript , may also express the long form of the leptin receptor providing a neurochemical basis for observations of interactions between cholecystokinin and leptin . - pubmed
Administration of Y-27632 moderately suppressed the atrioventricular nodal conduction , slightly but significantly accelerated the repolarization process , and potently increased the coronary blood flow , whereas it hardly affected the intraventricular conduction . - pubmed
The estimated concentrations of Y-27632 causing the currently observed effects were enough to inhibit Rho - kinase . - pubmed
Activation of protein kinase C ( PKC ) by perfusion of hearts with phorbol-12 - myristate-13 - acetate ( PMA ) or endothelin-1 ( ET-1 ) inhibited the maximum ATPase rate by up to 25 % and increased the Ca2 + sensitivity of ATPase activity and of isometric tension by up to 0.15 pCa units . - pubmed
For comparison , the beta - adrenergic agonist isoproterenol caused a 38 % increase in maximum ATPase rate and a 0.12 pCa unit decrease in myofilament Ca2 + sensitivity . - pubmed
A primary role of PKC phosphorylation of cTnI may be to reduce the requirements of the contractile apparatus for both Ca2 + and ATP , thereby promoting efficient ATP utilisation during contraction . - pubmed
Low levels of apolipoprotein A1 ( ApoA1 ) and cholesterol in the serum and undetectable levels of high density lipoprotein ( HDL ) and low density lipoprotein ( LDL ) cholesterol in the blood confirmed the diagnosis of TD . - pubmed
Here we report that the receptor tyrosine kinases Ror1 and Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons . - pubmed
Upon Ror1 or Ror2 suppression using antisense oligonucleotides or RNA interference ( RNAi ) , neurons extended shorter and less branched minor processes when compared to those in control cells . - pubmed
Bcl-2 enhances Ca ( 2 + ) signaling to support the intrinsic regenerative capacity of CNS axons . - pubmed
Here , we report that Bcl-2 supports axonal growth by enhancing intracellular Ca ( 2 + ) signaling and activating cAMP response element binding protein ( CREB ) and extracellular - regulated kinase ( Erk ) , which stimulate the regenerative response and neuritogenesis . - pubmed
Expression of Bcl-2 decreases endoplasmic reticulum ( ER ) Ca ( 2 + ) uptake and storage , and thereby leads to a larger intracellular Ca ( 2 + ) response induced by Ca ( 2 + ) influx or axotomy in Bcl-2 - expressing neurons than in control neurons . - pubmed
Bcl - x ( L ) , an antiapoptotic member of the Bcl-2 family that does not affect ER Ca ( 2 + ) uptake , supports neuronal survival but can not activate CREB and Erk or promote axon regeneration . - pubmed
These results suggest a novel role for ER Ca ( 2 + ) in the regulation of neuronal response to injury and define a dedicated signaling event through which Bcl-2 supports CNS regeneration . - pubmed
The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4 - N - acetylmuramidase ( lysozyme ) domain in the C - terminal portion . - pubmed
Sodium dodecyl sulfate extracts of strain Xc showed two major bacteriolytic bands with molecular masses of 107 and 79 kDa , both of which were absent from a mutant with inactivated atlA. - pubmed
Because ADX reduces the orexigenic actions of NPY and AgRP , we hypothesised that ADX would also reduce the orexigenic action of ghrelin . - pubmed
Chronic alcohol exposure resulted in significant NTS gene regulation related to the general processes of synaptic transmission , intracellular signaling , and cation transport as well as specific neuronal functions including plasticity and seizure behavior that could be related to alcohol withdrawal symptoms . - pubmed
The differentially expressed genes were also significantly enriched for enzymes of lipid metabolism , glucose metabolism , oxidative phosphorylation , MAP kinase signaling , and calcium signaling pathways from KEGG . - pubmed
Intriguingly , many of the genes we found to be differentially expressed in the NTS are known to be involved in alcohol - induced oxidative stress and/or cell death . - pubmed
The study provides evidence of very extensive alterations of physiological gene expression in the NTS in the adapted state to chronic alcohol exposure . - pubmed
Polyamine oxidase and acrolein as novel biochemical markers for diagnosis of cerebral stroke . - pubmed
BACKGROUND AND PURPOSE : We found previously that plasma levels of acrolein ( CH2 = CHCHO ) and spermine oxidase ( SMO ) were well correlated with the degree of severity of chronic renal failure . - pubmed
The size of stroke was nearly parallel with the multiplied value of acrolein and total polyamine oxidase ( AcPAO plus SMO ) . - pubmed
However , the caspase inhibitors did not affect the DeltaPsim , but cyclosporin A inhibited the collapse of DeltaPsim followed by a reduction of cell death . - pubmed
Lidocaine - induced apoptosis was mitochondria- and caspase - dependent , but the collapse of DeltaPsim was independent of caspase activation . - pubmed
Pretreatment with PRE-084 , a sigma1 receptor agonist , resulted in significant increased viability in a dose - related manner . - pubmed
The effects of PRE-084 were antagonized by pretreatment with sigma1 receptor antagonists ( NE-100 and BD1047 ) , but interestingly were synergized by cotreatment with BD1047 that also presented an affinity for the sigma2 receptor . - pubmed
EFFECT OF 2,4 - DICHLOROPHENOXYACETIC ACID ON PROTEOLYTIC ACTIVITY OF RED KIDNEY BEAN PLANTS . - pubmed
After separating tRNAs on a two - dimensional polyacrylamide gel , the positions of seven selected tRNAs were identified by Northern hybridization and their relative affinities to immobilized exportin - t x ran x GppNHp complex were estimated in the order of tRNA(Leu)(CAG ) & gt ; tRNA(Ser)(GCU ) , tRNA(Leu)(CAA ) , tRNA(Ser)(UGA ) & gt ; tRNA(Ser)(AGA ) , tRNA(Leu)(AAG ) & gt ; tRNA(Arg)(ACG ) . - pubmed
In multivariable analyses and adjusting for established risk factors and additionally for glomerular filtration rate and for hemoglobin , serum albumin , proteinuria , and C - reactive protein levels , a higher level of serum phosphorus was associated with an increased CVD risk in a continuous fashion ( adjusted hazard ratio per increment of milligrams per deciliter , 1.31 ; 95 % confidence interval , 1.05 - 1.63 ; P=.02 ; P value for trend across quartiles = .004 ) . - pubmed
A phase II placebo - controlled trial of neoadjuvant anastrozole alone or with gefitinib in early breast cancer . - pubmed
We have therefore investigated whether neoadjuvant gefitinib , an EGFR inhibitor , might overcome biologic and clinical resistance to neoadjuvant anastrozole in a phase II placebo - controlled trial . - pubmed
ORs in the combination and anastrozole alone groups were 48 % and 61 % ( estimated difference = -13.1 % ; 95 % CI , -27.3 % to 1.2 % ) , respectively , with a nonsignificant trend against the combination ( P = .08 ) and 48 % versus 72 % ( estimated difference = -24.1 % ; 95 % CI , -45.3 % to -2.9 % ) in the progesterone - receptor - positive subgroup , which was significant ( P = .03 ) and consistent with Ki67 changes . - pubmed
Some proteins first enter the endocytic pathway before appearing at the cell surface ; this is detected by treating the cells at 4 degrees and 37 degrees C. Analysis of the number of sialic acids on proteins of cells treated at 4 degrees C identifies proteins resident at the cell surface , while cells treated at 37 degrees C internalize the sialidase , which can then act with proteins in the endocytic compartments . - pubmed
As shown by both methods , APE1 binds with the highest efficiency to DNA substrate containing 5 -sugar phosphate group in the nick / gap , whereas DNA polymerase beta binds to DNA duplex with a mononucleotide gap flanked by the 5 -p group . - pubmed
When APE1 and DNA polymerase beta are both present , a ternary complex APE1 - DNA polymerase beta - DNA is formed with the highest efficiency with DNA product of APE1 endonuclease activity and with DNA containing 5 -flap or mononucleotide - gapped DNA with 5 -p group . - pubmed
Kinetic deuterium isotope effect analysis and Hammett analysis using a series of phenol derivatives under anaerobic conditions ( single - turnover reaction ) have indicated that the monooxygenation reaction of phenols to catechols by the peroxo species of oxyhemocyanin proceeds via electrophilic aromatic substitution mechanism as in the case of tyrosinase . - pubmed
The HLA - B*3713 allele differs from the closest matching allele B*370101 by one nucleotide substitutions in exon 3 at nt 527(T-->A ) , resulting in an amino acid change from Val ( GTG ) to Glu ( GAG ) at codon 152 . - pubmed
Intranasal administration of ovalbumin ( OVA ) formulated in an archaeal lipid mucosal vaccine adjuvant and delivery ( AMVAD ) system prepared by the addition of CaCl2 to small unilamellar archaeosomes ( liposomes made from archaeal polar lipids ) containing encapsulated OVA , was recently shown to elicit strong and sustained OVA - specific mucosal and systemic immune responses . - pubmed
The D - lactate dehydrogenase is a zinc - flavoprotein with high acceptor specificity for cytochrome c , that catalyzes the oxidation of D - lactate into pyruvate . - pubmed
New oxidant used for the post - column derivatization determination of Malachite Green and Leucomalachite Green residues in cultured aquatic products by high - performance liquid chromatography . - pubmed
When the eluate was combined with 3.0 x 10(-4 ) mol L(-1 ) iodine solution , LMG was converted to MG and detected at 618 nm after post - column derivatization . - pubmed
Seven peptides , Val - Asp - Phe ( IC(50 ): 6.59 microM ) , Leu - Pro - Phe ( 10.59 microM ) , Met - Pro - Phe ( 17.98 microM ) , Tyr - Thr - Ala - Gly - Val ( 23.38 microM ) , Glu - Arg - Tyr - Pro - Ile ( 8.76 microM ) , Ile - Pro - Phe ( 8.78 microM ) , and Thr - Thr - Ile ( 24.94 microM ) , were identified by liquid chromatography - mass spectrometry ( LC - MS / MS ) , and their IC(50 ) values were predicted by using our previously reported structure and activity models . - pubmed
The fruit samples gained from the field trials were quantitatively analysed by gas chromatography with a nitrogen phosphorus detector ( GC / NPD ) for tebuconazole residues . - pubmed
RESULTS : Tebuconazole residues in the fruits gained from the plot treated with the WP formulation , 14 days after application , were in the range 0.01 - 0.07 mg kg(-1 ) , while corresponding residues in the plot treated with the WG formulation were in the range 0.01 - 0.06 mg kg(-1 ) . - pubmed
CONCLUSION : No significant differences in the residue levels of tebuconazole could be observed between the trials conducted with the WP and the WG formulation . - pubmed
Specific salivary components were lactoferrin , secretory IgA , albumin , lysozyme , mucin , cystatin , K+ , Ca2 + , Na + and Cl- . - pubmed
RESULTS : Significant associations were found between caries , age and specific individual submandibular / sublingual salivary proteins ( lactoferrin , albumin , lysozyme , mucin and cystatin ) and specific inorganic constituents ( K+ , Ca2 + , Na+ and Cl- ) . - pubmed
Steroid - resistant acute rejection ( SR - AR ) and ductopenic rejection ( DR ) after liver transplantation are infrequent , but difficult to manage . - pubmed
We conclude that a dual therapy regimen of tacrolimus and sirolimus can achieve a high response rate as a rescue therapy for SR - AR and DR , provided it is begun as soon as possible . - pubmed
Vasopressin plays an important role in the control of blood osmolarity and vascular tone , but is also involved in many other physiological events , which are mediated mainly via three types of vasopressin receptor : V1R , V2R , and V3R. - pubmed
V1R primarily mediate the vascular , and V2R the aquaretic , effects of vasopressin . - pubmed
Vasopressin may also interact with other receptors , like adrenergic and angiotensin - II receptors , or with distinct biological pathways , including those of nitric oxide and the K(ATP ) channel . - pubmed
Currently , vasopressin and terlipressin are most commonly used to stimulate V1R in vasodilatory shock and cardiac arrest , while desmopressin , a synthetic analogue of vasopressin , acts on V2R ; but new molecules are becoming available in the treatment of inappropriate antidiuretic hormone ( ADH ) secretion . - pubmed
On the catecholamine control of growth hormone regulation . - pubmed
INTERPRETATION & amp ; CONCLUSION : Our study showed high ESBL occurrence with CTX - M as the emerging type in our hospital and CTX - M-3 being reported for the fi rst time in India . - pubmed
Gallic acid induces neuronal cell death through activation of c - Jun N - terminal kinase and downregulation of Bcl-2 . - pubmed
Gallic acid also induced cleavage of poly ( ADP - ribose ) polymerase , which is strongly related to apoptosis in neurons . - pubmed
The objectives of this investigation were to assess the ability of gastrointestinal simulation software to predict the oral absorption of the BCS class I drugs propranolol and metoprolol and the BCS class III drugs cimetidine , atenolol , and amoxicillin , and to perform in silico bioequivalence studies to assess the feasibility of extending biowaivers to BCS class III drugs . - pubmed
For BCS class III drugs with intestinal absorption patterns similar to those of cimetidine , atenolol or amoxicillin , the dissolution criteria for extension of biowaivers to BCS class III drugs warrants further investigation . - pubmed
The substrate cleavage pattern monitored by capillary zone electrophoresis showed that TpAraF is a classical exo - acting enzyme producing arabinose as its end - product . - pubmed
Synthesis and pharmacological evaluation of fluorine - containing D3 dopamine receptor ligands . - pubmed
These compounds were also evaluated for intrinsic activity at D ( 2 ) and D ( 3 ) receptors using a forskolin - dependent adenylyl cyclase assay . - pubmed
Furthermore , compound 20e , which has the highest binding affinity and selectivity for the D ( 3 ) receptor ( K(i ) = 0.17 nM for D(3 ) , 163 - fold selectivity for D ( 3 ) vs D ( 2 ) receptors ) , represents a candidate fluorine-18 radiotracer for in vivo PET imaging studies on the regulation of D ( 3 ) receptor expression . - pubmed
Incorporation of interactions between two BCAs did not greatly affect the disease dynamics except when a mycoparasitic and , to a lesser extent , an antibiotic - producing BCA was involved . - pubmed
OBJECTIVES : Beryllium sensitisation ( BeS ) and chronic beryllium disease ( CBD ) are caused by exposure to beryllium with susceptibility affected by at least one well - studied genetic host factor , a glutamic acid residue at position 69 ( E69 ) of the HLA - DPbeta chain ( DPbetaE69 ) . - pubmed
To further unmask the mechanics governing extracellular dopamine levels we used intravenous injection of the vesicular monoamine transporter ( VMAT2 ) inhibitor , tetrabenazine , to deplete dopamine storage and increase cytoplasmic dopamine in the nerve terminals . - pubmed
Tetrabenazine almost abolished phasic dopamine release but increased extracellular dopamine to ~500 nM , presumably by inducing reverse transport by dopamine transporter ( DAT ) . - pubmed
Along the same way shown by imatinib in chronic myeloid leukemia , compounds that selectively target ALK are bringing a revolution in the treatment of ALK - positive tumors . - pubmed
Crizotinib has just been approved , and new more potent ALK inhibitors will shortly follow . - pubmed
Total cholesterol ( TC ) and triglyceride ( TG ) of plasma were tested using Toshiba 120 Automatic Biochemical Analyzer . - pubmed
There has been a longstanding interest in non - steroidal anti inflammatories in FAP where trials have shown regression of polyps with the " pro drug"sulindac and the selective COX2 inhibitors though impact on long - term cancer risk is not confirmed . - pubmed
For those who took aspirin ( or AP ) for a minimum of 2 years ( per protocol ) the HR was 0.41 ( CI 0.19 - 0.86 p = 0.02 ) and the IRR , 0.37 ( CI 0.18 - 0.78 p = 0.008 ) . - pubmed
tuberculosis - DNA gyrase apoprotein structures and topoII - DNA-6FQ complexes were used for defining the 6 - fluoroquinolones ( 6 - FQs ) binding pockets . - pubmed
We show that nonribosomal peptide synthetases ClbN and ClbB assemble and process an N - acyl - D - asparagine prodrug scaffold that serves as a substrate for the periplasmic D - amino peptidase ClbP. - pubmed
Lectin PHA - L Conjugates ( PHA - L , Invitrogen L - 11270 , ) was injected into the MVN as an anterograde tracer , and 5 % FluoSpheres carboxylate - modified microspheres ( MFS , Molecular Probe F-8793 ) was injected into the contralateral peripheral vestibule using as a retrograde tracer . - pubmed
We describe five genes , uricase , 5 - hydroxyisourate hydrolase , parahox neighbor B , allantoinase , and allantoicase , each coding for one of the five enzymes involved in urate degradation to urea , and report the identification of a putative second form of 5 - hydroxyisourate hydrolase that is characteristic of the genus Latimeria . - pubmed
Changes in systolic blood pressure ( SBP ) and diastolic blood pressure ( DBP ) were examined by randomization arm ( intensive lifestyle change [ ILS ] , metformin 850 mg twice daily , or placebo ) . - pubmed
Associations between changes in blood pressure and changes in sex hormone - binding globulin , estradiol , testosterone , and dehydroepiandrosterone were also examined . - pubmed
Estrogen users and nonusers randomized to ILS had similar decreases in SBP ( -3.3 vs -4.7 mm Hg , P = 0.45 ) and DBP ( -3.1 vs -4.7 mm Hg , P = 0.16 ) . - pubmed
Also , alcohol use in women decreases the success of infertility treatment , such as in vitro fertilization ( IVF ) . - pubmed
Our goal was to determine if there were differences in IVF outcomes and alcohol use parameters among at - risk drinkers randomized to a brief intervention ( BI ) versus assessment only ( AO ) . - pubmed
CONCLUSIONS : BI and AO contributed to a decrease in alcohol use and did not demonstrate differences in IVF outcomes . - pubmed
Antitumor activity of the selective pan - RAF inhibitor TAK-632 in BRAF inhibitor - resistant melanoma . - pubmed
Recently , the BRAF inhibitors vemurafenib and dabrafenib have emerged as promising agents for the treatment of melanoma patients with BRAF - activating mutations . - pubmed
Here , we demonstrate that the selective pan - RAF inhibitor TAK-632 suppresses RAF activity in BRAF wild - type cells with minimal RAF paradoxical activation . - pubmed
Our analysis using RNAi and TAK-632 in preclinical models reveals that the MAPK pathway of NRAS - mutated melanoma cells is highly dependent on RAF . - pubmed
We also show that TAK-632 induces RAF dimerization but inhibits the kinase activity of the RAF dimer , probably because of its slow dissociation from RAF . - pubmed
As a result , TAK-632 demonstrates potent antiproliferative effects both on NRAS - mutated melanoma cells and BRAF - mutated melanoma cells with acquired resistance to BRAF inhibitors through NRAS mutation or BRAF truncation . - pubmed
Furthermore , we demonstrate that the combination of TAK-632 and the MAPK kinase ( MEK ) inhibitor TAK-733 exhibits synergistic antiproliferative effects on these cells . - pubmed
Our findings characterize the unique features of TAK-632 as a pan - RAF inhibitor and provide rationale for its further investigation in NRAS - mutated melanoma and a subset of BRAF - mutated melanomas refractory to BRAF inhibitors . - pubmed
Polyacrylamide - gel electrophoresis showed the existence of nine isoenzymes of SOD . - pubmed
Results showed that , precipitation pH value increased from spring to summer , soluble Cd concentration of spring runoff was significantly higher than that of summer rainfall runoff , which presented an obviously seasonal heterogeneity and had a negative correlation with rainfall pH value , and rainfall pH value can obviously impact soil soluble Cd transportation into surface runoff charge . - pubmed
Skeletal muscle is an important target tissue for adiponectin where it regulates glucose and fatty acid metabolism directly and via insulin sensitizing effects . - pubmed
On the treated side , CBF and CBV values were higher before and 7 days after cranioplasty than at 3 months after surgery , whereas MTT values were lower at 7 days than at 3 months after surgical treatment . - pubmed
In this study , we demonstrate that deletion of the RR - encoding gene , abrC3 ( SCO4596 ) , results in a dramatic decrease in actinorhodin ( ACT ) and undecylprodiginine ( RED ) production and delays morphological development . - pubmed
Characterization of the mode of action of isohymenialdisine revealed that it directly acts on PKR by inhibiting autophosphorylation , perturbs the PKR - eIF2alpha phosphorylation axis , and can be modeled into the PKR ATP binding site . - pubmed
Conversely , amino acid exchanges in the two GARRPR motifs of Bag-1L can impair the interaction between Bag-1L and AR without altering the ability of Bag-1L to bind to chromatin . - pubmed
Furthermore , the mutant Bag-1L increases androgen - dependent activation of a subset of AR targets in a genome - wide transcriptome analysis , demonstrating a repressive function of the GARRPR / BF-3 interaction . - pubmed
However , RAGE deletion did not completely prevent inflammation or vascular damage , possibly because the induction of mitochondrial oxidative stress by dicarbonyls also contributes to inflammation and atherogenesis . - pubmed
Extrasynaptic glutamate release through cystine / glutamate antiporter contributes to ischemic damage . - pubmed
During brain ischemia , an excessive release of glutamate triggers neuronal death through the overactivation of NMDA receptors ( NMDARs ) ; however , the underlying pathways that alter glutamate homeostasis and whether synaptic or extrasynaptic sites are responsible for excess glutamate remain controversial . - pubmed
Blockade of excitatory amino acid transporters or vesicular glutamate release did not inhibit ischemia - gated currents or neuronal damage after OGD . - pubmed
Furthermore , glutamate released by the cystine / glutamate antiporter activated extrasynaptic , but not synaptic , NMDARs , and blockade of extrasynaptic NMDARs reduced ischemia - gated currents and cell damage after OGD . - pubmed
The aryl hydrocarbon receptor : a review of its role in the physiology and pathology of the integument and its relationship to the tryptophan metabolism . - pubmed
The aryl hydrocarbon receptor ( AHR ) is a cytosolic receptor for low molecular weight molecules , of which the most widely recognized ligand is 2,3,7,8 - tetrachlorodibenzo - p - dioxin ( TCDD ) , and the most widely recognized effect , chloracne . - pubmed
I provide a review outlining the role AHR plays in the development , cellular oxidation / antioxidation , responses to ultraviolet light , melanogenesis , epidermal barrier function , and immune regulation and its relationship to tryptophan metabolism . - pubmed
MBT also decreased triacylglycerol and total cholesterol levels in the muscle by 30 % , which was correlated with suppression of lipogenic genes such as ACC , C / EBP alpha , PGC-1 alpha , and PPAR gamma . - pubmed
In particular , decreased levels of p - ERK1 / 2 , PPAR gamma , and C / EBP alpha in the MBT - treated groups suggest that MBT might inhibit adipogenesis and decrease differentiation via the MEK / ERK pathway . - pubmed
Fasting glucose before breakfast on workdays ( after 7.1 + - 0.8 h ) was not significantly different between the three interventions ( CS 4.1 + - 0.5 mmol / l , MCS 4.6 + - 0.7 mmol / l , pasta 4.3 + - 0.9 mmol / l ) . - pubmed
In this sense , we construct three indices including Net Global Warming Potential ( NGWP ) and Nitrogen Surplus ( NS ) , simulated by a biogeochemistry model , and also an Environmental Impact Quotient ( EQI ) for paddy fields . - pubmed
Moreover , rutin decreased macrophage infiltration , proinflammatory cytokine expression and phosphorylation of nuclear factor - kappaB p65 . - pubmed
Furthermore , rutin inhibited extracellular matrix accumulation by reducing expression of type I / III collagen and fibronectin . - pubmed
Rutin also prevented the epithelial - mesenchymal transition processes of renal tubular cells by decreasing alpha - smooth muscle actin expression and retaining E - cadherin expression . - pubmed
These effects of rutin were in parallel with the reductions in Smad3 activity and pivotal to the fibrogenic potential of TGF - beta1 . - pubmed
Taken together , the renoprotective effects of rutin in obstructive nephropathy were likely due to anti - inflammatory effects and inhibition of TGF - beta1 / Smad3 signaling . - pubmed
Secondary outcome measures were tumour size , nodal positivity and radioactive iodine ablation rate . - pubmed
A reduced graphene oxide - based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2 . - pubmed
A novel fluorescence nanoprobe ( reduced nano - graphene oxide [ nrGO]/fluorescein isothiocyanate - labeled peptide [ Pep - FITC ] ) for ultrasensitive detection of matrix metalloproteinase 2 ( MMP2 ) has been developed by engineering the Pep - FITC comprising the specific MMP2 substrate domain ( PLGVR ) onto the surface of nrGO particles through non - covalent linkage . - pubmed
Upon cleavage of the amide bond between Leu and Gly in the Pep - FITC by protease - MMP2 , the FITC bound to nrGO was separated from nrGO surface , disrupting the fluorescence resonance energy transfer process and resulting in fluorescence recovery of FITC . - pubmed
Under optimal conditions , the fluorescence recovery of nrGO / Pep - FITC was found to be directly proportional to the concentration of MMP2 within 0.02 - 0.1 nM. - pubmed
The X - ray crystallographic analysis confirms that while CIP and CIT manifest planar structures , DPT shows a curved pi - conjugated carbon skeleton . - pubmed
In this study , we found that HSP90 was cleaved to a 55kDa protein after treatment with proteasome inhibitors including MG132 in leukemia cells but was not cleaved in other tissue - derived cells . - pubmed
HSP90 has two major isoforms ( HSP90alpha and HSP90beta ) , and both were cleaved by MG132 treatment . - pubmed
MG132 treatment also induced a decrease in HSP90 client proteins . - pubmed
MG132 treatment generated ROS , and the cleavage of HSP90 was blocked by a ROS scavenger , N - acetylcysteine ( NAC ) . - pubmed
MG132 activated several caspases , and the activation was reduced by pretreatment with NAC . - pubmed
Based on an inhibitor study , the cleavage of HSP90 induced by MG132 was dependent on caspase 10 activation . - pubmed
MG132 upregulated VDUP-1 expression and reduced the GSH levels implying that the regulation of redox - related proteins is involved . - pubmed
Taken all together , our results suggest that the cleavage of HSP90 by MG132 treatment is mediated by ROS generation and caspase 10 activation . - pubmed
Our results indicated that PcTx1can mainly regulate ASIC1a gating process through hydrogen bonds , which can affect their relative positions of several key domains in ASIC1a , further , a long - range conformational changes path was determined , which is composed of beta1 , beta2 , beta10 , alpha6 , alpha7 , beta11 , and beta12 in ASIC1a . - pubmed
It is reported that tert - butylhydroquinone ( tBHQ ) , a nuclear factor erythroid 2 - related factor 2(Nrf2)-inducer , has a variety of pharmacological activities such as anti - oxidation and anti - inflammatory effect . - pubmed
It increased PVN level of reactive oxygen species , gp91phox , IL-1beta , IL-6 , p - IKKbeta and nuclear factor - kappa B ( NF - kappaB ) activity , decreased PVN level of Nrf2 and Cu / Zn - SOD . - pubmed
In this paper , the prediction success of DDIs involving various cytochrome P450 isoenzyme ( CYP ) modulators namely ketoconazole ( a competitive inhibitor of CYP3A ) , itraconazole ( a competitive inhibitor of CYP3A ) , clarithromycin ( a mechanism - based inhibitor of CYP3A ) , quinidine ( a competitive inhibitor of CYP2D6 ) , paroxetine ( a mechanism - based inhibitor of CYP2D6 ) , ciprofloxacin ( a competitive inhibitor of CYP1A2 ) , fluconazole ( a competitive inhibitor of CYP2C9/2C19 ) and rifampicin ( an inducer of CYP3A ) were assessed using Simcyp software . - pubmed
Yet , it ranges from & lt;1 % to nearly 100 % compared to AUG translation , depending on surrounding sequences , including Kozak , and possibly additional nucleotide contexts . - pubmed
5MP represses non - AUG translation by competing with eIF5 for the Met - tRNAi - binding factor eIF2 . - pubmed
Consistently , eIF5 increases , whereas 5MP decreases translation of NAT1 / EIF4G2 / DAP5 , whose sole start codon is GUG . - pubmed
Furthermore , GNF - SERS combined with quantum chemical calculation identified cysteine - derived glutathione and hypotaurine ( HT ) as tumour - dominant and parenchyma - dominant metabolites , respectively . - pubmed
CD44 knockdown in cancer diminished glutathione , but not HT in tumours . - pubmed
Mechanisms whereby tumours sustained HT under CD44 - knockdown conditions include upregulation of PHGDH , PSAT1 and PSPH that drove glycolysis - dependent activation of serine / glycine - cleavage systems to provide one - methyl group for HT synthesis . - pubmed
HT was rapidly converted into taurine in cancer cells , suggesting that HT is a robust anti - oxidant for their survival under glutathione - suppressed conditions . - pubmed
After 7 , 14 , and 30 days post - surgery , a fragment of each specimen was fixed in - 80 C liquid nitrogen for zymographic evaluation , while the remaining was fixed in 10 % formalin for histological birefringence analysis . - pubmed
We report here the three - dimensional structure of McpU - LBD in complex with putrescine at a resolution of 2.4 A , which fitted well a solution structure generated by small - angle X - ray scattering . - pubmed
Putrescine bound to a negatively charged pocket in the membrane distal module of McpU - LBD . - pubmed
Synthesis , in vitro antiproliferative activity , and kinase inhibitory effects of pyrazole - containing diarylureas and diarylamides . - pubmed
The crystalline phases of these materials have PE chromophores that are twisted due to non - covalent perfluoroarene - arene ( ArF - ArH ) interactions involving perfluorophenyl pendants and the terminal rings of the PE chromophore , resulting in interrupted conjugation and an absence of chromophore aggregation . - pubmed
Here , we present a surface - enhanced Raman scattering ( SERS ) platform made by large - scale self - assembly of Au nanoparticle ( NP ) arrays at the cyclohexane / water interface and its use for pesticides residues trace detection . - pubmed
Transmission electron microscopy ( TEM ) images demonstrate a good uniformity of interparticle distances ( 2 - 3 nm ) in the Au NP arrays . - pubmed
However , upon 1 h of ultrasonic processing at 80 C , a significant increase of oxidation was observed with high peroxide and p - anisidine values in the post - processed oils ; meanwhile , the amount of unsaturated fatty acids decreased as well . - pubmed
Photolysis separates the taxane from the B12 cytoplasmic anchor , enabling the drug to exit the RBC carrier . - pubmed
A covalently appended Cy5 antenna sensitizes the conjugate ( Cy5 - B12 - TAX ) to far red light , thereby circumventing the intense light absorbing properties of hemoglobin ( 350 - 600 nm ) . - pubmed
Microscopy and imaging flow cytometry reveal that Cy5 - B12 - TAX - loaded mRBCs act as drug carriers . - pubmed
The isolate showed hydrocarbon degrading enzyme activities namely alkane hydroxylase , catechol 1,2 - dioxygenase and catechol 2,3 - dioxygenase . - pubmed
The level of C23O activity was 9.63 times higher than C12O thus suggesting active involvement of meta - cleavage pathway . - pubmed
Importantly , USP7 small - molecule inhibitors , P5091 and P22077 , inhibit SOCS1 expression and enhance IFN - I antiviral efficacy . - pubmed
We have evaluated the toxicity of flutianil , which was developed by OAT Agrio . - pubmed
These include promotion of mucosal antibody and anti - microbial peptide production , coupled with a strong suppression of inflammatory cytokines and reactive oxygen species . - pubmed
Pyruvate kinase activity and lactate detection assays were performed to analyze the effects of Sam68 on aerobic glycolysis . - pubmed
Sam68 significantly promoted cell proliferation and caused a decrease of PKM1 / PKM2 ratio , resulting in the metabolism of glucose switched from oxidative phosphorylation to glycolysis in CRC cells . - pubmed
Besides , Sam68 enhanced PKM2 mRNA transport from the nucleus to cytoplasm and increased the expression of PKM2 protein , resulting in elevated pyruvate kinase activity and lactate production . - pubmed
sanctori ) , harvested from the Algerian coast , we aimed to study their proximate composition , fatty acid profile and angiotensin - converting enzyme ( ACE ) inhibitory activity . - pubmed
The four holothurian species showed a high amount of protein ( 49.26 - 69.34 % ) , and an impressive lipid profile of 27 fatty acids , mainly composed of polar fatty acids ( 91.16 - 93.85 % ) , with a high polyunsaturated fatty acids ( PUFA ) content ( 50.90 - 71.80 % ) , particularly eicosapentaenoic acid ( EPA ) ( 5.07 - 8.76 % ) and docosahexaenoic acid ( DHA ) ( 4.86 - 7.25 % ) . - pubmed
Anti - tumor and Phenotypic Regulation Effect of Matrine on Dendritic Cells through Regulating TLRs Pathway . - pubmed
And the killing effect of DAK were measured by MTT assay . - pubmed
RESULTS : Matrine significantly increased the mRNA expression of TLR7 , TLR8 , myeloid differentiation factor 88 ( MyD88 ) , tumor necrosis factor receptor - associated factor 6 ( TRAF-6 ) and I kappa B kinase ( IKK ) , as well as the protein expression of TLR7 and TLR8 , and up - regulated the levels of interleukin-12 ( IL-12 ) , IL-6 and tumor necrosis factor - alpha ( TNF - alpha ) , meanwhile , it also increased the expressions of MHC - II , CD54 , CD80 and CD86 in DCs . - pubmed
CONCLUSION : Matrine plays an anti - tumor role by regulating TLRs signal transduction pathway , promoting the secretion of inflammatory cytokines and enhancing immune function . - pubmed
Mineral carbonation routes have been extensively studied for almost two decades at Abo Akademi University , focusing on the extraction of magnesium from magnesium silicates using ammonium sulfate ( AS ) and/or ammonium bisulfate ( ABS ) flux salt followed by carbonation . - pubmed
This study focused on the separation of AS , ABS and aqueous ammonia using different setups of bipolar membrane electrodialysis using both synthetic and rock - derived solutions . - pubmed
ORX - CFW and ORX - MBW partially prevented the reduction of serum testosterone concentrations and decreased insulin resistance , compared to the ORX - CON . - pubmed
Affinity labeling of gamma - glutamyl transpeptidase and location of the gamma - glutamyl binding site on the light subunit . - pubmed
Gamma - Glutamyl transpeptidase , which consists of two nonidentical subunits , is rapidly inactivated with respect to its transpeptidase and hydrolase activities by the gamma - glutamyl analogs 6 - diazo-5 - oxo - L - norleucine and L - azaserine . - pubmed
Inactivation , which is prevented by gamma - glutamyl substrates ( but not by acceptor substrates ) , is accelerated by maleate , which was previously shown to enhance utilization of glutamine by transpeptidase . - pubmed
The findings , which indicate that these enzymes have similar gamma - glutamyl binding subunits , are relevant to the structure - function relationships of this membrane - bound enzyme and its physiological role . - pubmed
The changes observed during the estrous cycle and after castration suggest that gonadal steroids play a direct role in the control of hypothalamic Gn - RH . - pubmed
By virtue of the conformational rigidity of the decalols , possible inferences concerning the stereochemical aspects of the interaction of the ephedrine analogs with adrenergic neurone - receptor sites are discussed . - pubmed
Effects of naltrindole and nor - binaltorphimine treatment on antinociception induced by sub - acute selective mu opioid receptor blockade . - pubmed
Cypridina luciferin analog ( CLA ) dependent chemiluminescence was used to detect superoxide anion radicals . - pubmed
The Type I ( mineralocorticoid ) receptor has identical affinities in vitro for cortisol and aldosterone . - pubmed
It has been suggested that the selective role of aldosterone in regulating sodium homeostasis relies on the microsomal enzyme 11 beta - hydroxysteroid dehydrogenase ( 11 - HSD ) . - pubmed
Gpp ( NH ) p and GTP gamma S inhibited phospholipase C at nanomolar concentrations . - pubmed
Increasing concentrations of Gpp ( NH ) p and GTP gamma S reversed the inhibitory effects and stimulated phospholipase C activity . - pubmed
Preincubation of coated vesicles with pertussis toxin blocked the poorly - hydrolyzable GTP - analogs ' inhibitory effects on phospholipase C. - pubmed
These data indicate that guanine nucleotides exert a dual regulatory control of phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a pertussis toxin - sensitive G - protein . - pubmed
Thyroglobulin antibody and thyroid peroxidase antibody were measured by highly sensitive assays which are based on the direct interaction between antibody and radiolabelled antigen . - pubmed
Our findings suggest a physiological circadian rhythm of spontaneous and LPS - induced IL-1 production of monocytes , independent of physiological cortisol levels , whereas unphysiological amounts of endogenous and exogenous glucocorticoids cause a substantial inhibition of IL-1 production with a latency time of 8 hours . - pubmed
Modulation of cytochrome oxidase activity by inorganic and organic phosphate . - pubmed
The activity of cytochrome oxidase reconstituted into phospholipid vesicles has been studied as a function of orthophosphate , ATP and inositol hexakisphosphate concentrations . - pubmed
This effect is related to a phosphate - dependent decrease in the rate constant for ferrocytochrome c oxidation observed in the presence of ionophores . - pubmed
The data can not be interpreted simply on the basis of ionic strength , which is known to limit cytochrome c binding to cytochrome oxidase , since cytochrome oxidase - containing vesicles responded differently to phosphate depending on the energization state of the phospholipid membrane . - pubmed
Treatment with lovastatin and cholestyramine can inhibit the direct synthesis pathway in this species , thereby lowering LDL apo B concentrations . - pubmed
The possibility that the direct synthesis pathway was confined to a specific subclass of LDL and the effect of lovastatin and cholestyramine on the metabolism of LDL subfractions were also investigated . - pubmed
Kinetic analysis of apo B specific activity curves following lovastatin treatment indicated that LDL apo B pool size was decreased by 25 % ( P less than .025 ) , which was due entirely to a 70 % ( P less than .025 ) decrease in the direct synthesis of LDL apo B , since VLDL - derived apo B , and LDL fractional catabolic rate ( FCR ) were not affected . - pubmed
T helper cell interactions with " presenting " cells and " target " B lymphocytes are inhibited by monoclonal antibodies directed either to the hapten or to I - A molecules on target cells . - pubmed
Carbachol induces desensitization of cardiac beta - adrenergic receptors through muscarinic M1 receptors . - pubmed
Incubation of enzymatically dissociated cardiac myocytes with carbachol leads to a time- and concentration - dependent loss of beta - receptors assayable with [ 3H ] CGP-12177 . - pubmed
The carbachol effects are not influenced by gallamine or oxotremorine which interact with the high - affinity ( M2 ) muscarinic receptors . - pubmed
These results suggest that carbachol - induced desensitization is secondary to activation of protein kinase C by diacylglycerols generated through M1 receptor - linked phosphoinositide hydrolysis . - pubmed
Timolol inhibits adenylate cyclase activity in the iris - ciliary body and trabecular meshwork of the eye and blocks activation of the enzyme by salbutamol . - pubmed
It is suggested that the hypotensive action of timolol on intraocular pressure results from structural and functional changes induced on the plasma membranes of the iris - ciliary body and trabecular meshwork by the thiadiazole group of the molecule , and , also , from the occupation of the adrenergic receptors of the iris - ciliary body by the tert - butylamino-2 - hydroxypropoxy part of the compound . - pubmed
The authors ' approach to the design of DNA polymerase - specific inhibitors , an approach based on the mechanism of action of 6 -( p - hydroxyphenylazo ) uracil , has been to disguise nucleic acid bases to mimic the purine substrates dGTP and dATP . - pubmed
Specifically , the strategy has been to synthesize bases with substituents that endow them with the capacity : to seek and react with unique features of the active site of a polymerase ; and to form H bonds with complementary template pyrimidines apposing the active site . - pubmed
This strategy has yielded a series of novel , enzyme - specific dATP and dGTP analogues which are non - polymerizable and which inhibit their target polymerase by sequestering it to a complementary pyrimidine residue in primer : template . - pubmed
Principles established with the pyrimidine derivatives have led to the development of bona fide purine nucleotide analogues which act as DNA polymerase inhibitors of high selectivity and unprecedented potency . - pubmed
For example , BuPdGTP , the 2 '- deoxyribonucleoside 5 '- triphosphate of N2 -( p - n - butylphenyl ) guanine ( BuPG ) , lacks discernible activity against mammalian polymerases beta and gamma , whereas it inhibits mammalian pol alpha with a Ki of less than 10 nanomolar . - pubmed
Currently , the authors are exploiting BuPdGTP , BuPdGDP , and similar butylanilino derivatives of dATP to probe the active site of pol alpha and to develop other N2 - substituted analogues which can bind selectively to the substrate sites of other important polymerases and nucleotide binding proteins . - pubmed
Effect of aluminum on parathyroid hormone secretion . - pubmed
In all these fusions , beta - galactosidase activity was shown to be sensitive to catabolite repression by glucose and to be inducible by polygalacturonate , galacturonate , and other intermediates of polygalacturonate catabolism . - pubmed
2 - Keto-3 - deoxygluconate appeared to be the true inducer of kduD expression resulting from galacturonate degradation . - pubmed
Characterization of the purple intermediate in the anaerobic reaction of D - amino - acid oxidase with D - alanine . - pubmed
To measure plasmin generation in these samples , we used the chromogenic plasmin substrate D - Val - Leu - Lys - p - nitroanilide , which liberates p - nitroaniline upon cleavage . - pubmed
The 21st chromosome has been implicated in the elevated activity of superoxide dismutase , a finding of significance in regard to potential intracellular damage from increased levels of peroxide . - pubmed
Amino acid sequence of a region in rabbit skeletal muscle glycogen synthase phosphorylated by cyclic AMP - dependent protein kinase . - pubmed
Purification of cardiac sarcolemmal vesicles : high sodium pump content and ATP - dependent , calmodulin - activated calcium uptake . - pubmed
The Ca2 + pump was activated by calmodulin . - pubmed
This activation was removed by addition of trifluoperazine , a specific inhibitor of calmodulin . - pubmed
When mean arterial pressure was decreased by 15 mmHg by adrenomedullin , the increases in heart rate and renal sympathetic nerve activity were 53 + /- 8 beats / min and 78 + /- 13 % , respectively , which were significantly smaller than those induced by intravenous injection of sodium nitroprusside ( 102 + /- 14 beats / min and 155 + /- 34 % , respectively ) . - pubmed
More than 86.0 % were resistant to ampicillin , and the addition of the beta - lactamase inhibitor sulbactam did not restore its activity . - pubmed
Hybridomas secreting IgG1 and IgG2b mAb , specific for the N - acetyl - glucosamine ( GlcNAc ) residues of streptococcal group A carbohydrate , were previously generated from a hybridoma secreting a mouse IgG3 mAb . - pubmed
Nevertheless , the IgG3 mAb binds more effectively than the V region - identical IgG1 or IgG2b mAb to each of three strains of group A streptococci that display different amounts of terminal GlcNAc residues on their cell walls . - pubmed
The magnitude of the subclass - associated differential in binding varies with the target strain , and , whereas the IgG3 mAb binds best to the strain expressing an intermediate amount of GlcNAc , the IgG1 and IgG2b mAb and IgG3 - derived F(ab')2 fragments bind best to the strain expressing the highest amount of GlcNAc . - pubmed
The IgG3 mAb also binds better than the IgG1 and IgG2b mAb to solid - phase GlcNAc50 - BSA , but the IgG2b mAb binds best to otherwise identical conjugates with lower ratios of GlcNAc to BSA ( 20:1 , 10:1 , 5:1 , and 1:1 ) . - pubmed
These results suggest that epitope density can significantly influence the magnitude of IgG subclass - associated binding differences , and that structural differences in the CH regions , particularly the CH2 and CH3 domains , can influence the apparent specificities of IgG molecules for multivalent Ag . - pubmed
Warfarin derivatives exert both their embryopathic and their fetopathic effects via pharmacologic action : inhibition of gamma carboxylation of osteocalcin and a similar carboxylation of the vitamin K dependent clotting factors . - pubmed
Administration of carbon tetrachloride ( CCl4 ) induced a significant decrease in liver regucalcin concentration and a corresponding elevation of serum regucalcin concentration at 24 h after the administration . - pubmed
Meanwhile , serum transaminases ( GOT and GPT ) activities were significantly increased by CCl4 administration , indicating that liver injury is induced . - pubmed
Substance abuse in patients was an independent inverse determinant of TC value ( p = 0.05 ) ; TG value correlated positively with alcohol use ( p & lt ; or = 0.1 ) and substance abuse ( p & lt ; 0.05 ) . - pubmed
Trunk LTM ( R2 = .37 ) , total - body fat tissue mass ( [ FTM ] R2 = .04 ) , androstenedione ( R2 = .03 ) , and total triiodothyronine ( [ T3 ] R2 = .02 ) were all significantly independently associated with REE . - pubmed
Together they explained 46 % ( model R2 ) of the interindividual variation in REE , with a standard error of estimate ( SEE ) of 549 kJ / d. We conclude that the interindividual variation in REE is explained mainly by differences in the visceral component of LTM , with additional minor information from the total - body FTM , androstenedione , and T3 . - pubmed
cerevisiae resembled that of bacteria ( reported earlier by us ) in several aspects : first , sulfate - S was used in preference to that of sulfonate , when both were present ; second , mutants unable to use sulfate as a source of sulfur because of deficiencies in ATP sulfurylase , adenylylsulfate kinase ( APS kinase ) or PAPS reductase were able to utilize sulfonates ; and third , mutants deficient in sulfite reductase were unable to utilize sulfonates . - pubmed
A subpopulation of cells express both N - cadherin and QH-1 antigen suggesting that endocardial and myocardial cells may arise from a common precursor population and that N - cadherin regulation may be a mechanism underlying specific cell sorting of these two cell populations during heart development . - pubmed
After G418 selection , the control transfectant clones transfected with pH8 vector not containing c - fos DNA insertion changed their original fibroblastic shape into dendritic cells . - pubmed
Inhibition of rat liver cytochrome P450 isozymes by isothiocyanates and their conjugates : a structure - activity relationship study . - pubmed
All isothiocyanates inhibited PROD more readily than EROD . - pubmed
Increases in the alkyl chain length of arylalkyl isothiocyanates to C6 resulted in an increased inhibitory potency in these assays ; at longer alkyl chain lengths ( C8 - C10 ) the inhibitory potency declined . - pubmed
The IC50s for phenethyl isothiocyanate ( PEITC ) were 47 , 46 and 1.8 microM for EROD , MROD and PROD , respectively . - pubmed
Substitution of an additional phenyl group on PEITC also increased the inhibitory potency ; the IC50s for 1,2 - diphenylethyl isothiocyanate ( 1,2 - DPEITC ) and 2,2 - diphenylethyl isothiocyanate ( 2,2 - DPEITC ) were 0.9 and 0.26 microM for EROD , and 0.045 and 0.13 microM for PROD , respectively . - pubmed
These results suggest the potential of isothiocyanates as structural probes for studying P450 isozymes . - pubmed
In addition , the inhibitory activity of isothiocyanates for PROD correlated with the previously demonstrated tumor inhibitory potency in ( 4 - methylnitrosamino )- 1 -( 3 - pyridyl )- 1 - butanone ( NNK ) induced A / J mouse lung tumor bioassays , which supports earlier findings that P450 2B1 is one of the major isozymes involved in NNK activation and that inhibition of this isozyme is an important mechanism for the chemopreventive activity of isothiocyanates . - pubmed
The magnitude of the dilution was in between that recorded for albumin and for sodium . - pubmed
It has been suggested that the biotransformation of oxybutynin is dependent on CYP3A. - pubmed
Because itraconazole , a widely used mycotic , is a potent inhibitor of CYP3A4 , we wanted to study a possible interaction between oxybutynin and itraconazole . - pubmed
CONCLUSIONS : Itraconazole moderately increases serum concentrations of oxybutynin , probably by inhibiting the CYP3A - mediated metabolism . - pubmed
Since about 90 % of the antimuscarinic activity of oxybutynin is attributable to N - desethyloxybutynin , any interaction of oxybutynin with CYP3A4 inhibiting drugs has only minor clinical significance . - pubmed
Recombinant fragment recognition by ICM - derived antibodies was delimited to three regions 150 - 200 residues long , with seven of the octapeptide epitopes also mapping to these regions . - pubmed
Morphine ( 10 mg / kg ) , D , L - amphetamine ( 4 mg / kg ) and riluzole ( 4 mg / kg ) itself each produced a significant CPP ; however , when riluzole was co - administered with morphine or amphetamine during the conditioning sessions , no CPP developed . - pubmed
The purpose of this study was to investigate the CYP isoform specificity of oxybutynin N - deethylation and possible interactions . - pubmed
Recombinant CYP3A5 enzyme had higher activity in oxybutynin N - deethylation than recombinant CYP3A4 . - pubmed
Ketoconazole inhibited oxybutynin N - deethylation by the recombinant CYP3A4 and CYP3A5 almost completely , whereas itraconazole inhibited the activity of CYP3A4 more potently than that of CYP3A5 . - pubmed
To investigate the properties of the two RAG1.RAG2 complexes in the presence of different Me2 + cofactors , we established an in vitro Mg2 + -based cleavage reaction on a single RSS . - pubmed
In contrast , Mn2 + allows aberrant binding of RAG1.RAG2 to single - stranded RSS and permits cleavage independent of binding to the nonamer . - pubmed
To determine the contribution of Me2 + ions at the early stages of V(D)J recombination , we analyzed specific DNA recognition and cleavage by RAG1.RAG2 on phosphorothioated substrates . - pubmed
These experiments revealed that Me2 + ions directly coordinate the binding of RAG1.RAG2 to the RSS DNA . - pubmed
All doses of bFGF used in this study suppressed synthesis of chondroitin 6 - sulfate . - pubmed
PKC alpha and PKC delta are activated and translocated to the nucleus upon exposure of cells to calcitriol . - pubmed
Accumulating evidence suggests that angiotensin -( 1 - 7 )( Ang -( 1 - 7 ) ) is an important component of the renin - angiotensin system and that the actions of the peptide may either contribute to or oppose those of Ang II . - pubmed
Both neprilysin and thimet oligopeptidase are also involved in the metabolism of bradykinin and the atrial natriuretic peptide . - pubmed
Moreover , recent studies suggest that in addition to Ang I and bradykinin , Ang -( 1 - 7 ) is an endogenous substrate for angiotensin converting enzyme . - pubmed
Thus , Ang -( 1 - 7 ) may oppose the actions of Ang II directly or as a result of increasing prostaglandins or nitric oxide . - pubmed
, glycation ) of proteins , which results in abnormal aggregation of collagen fibrils and production of superoxide radicals . - pubmed
The metabolite GLP-1 ( 9 - 36 ) amide was followed in blood , lung , and kidney . - pubmed
At 24 - h post - seeding interval , the number of cells which adhered to the ion - modified polystyrene was significantly higher than on the unmodified material ( +20 and +58 % in cultures with the polystyrene irradiated by lower and higher ion doses , respectively ) . - pubmed
As was shown by ELISA , the polystyrene irradiated by the higher ion dose enhanced the expression of a cytoskeletal protein , vimentin ( +65 % ) and protein of focal adhesion plaques , talin ( +15 % ) . - pubmed
TdT - mediated dUTP - biotin nick end - labeling ( TUNEL ) and enzyme histochemistry for acid phosphatase were performed in both models and results were analyzed by light microscopy , electron microscopy , and confocal laser scanning microscopy ( CLSM ) . - pubmed
jararacussu bothropstoxins - I ( BthTX - I ) and II ( BthTX - II ) were submitted to chemical modifications of : His , by 4 - bromophenacyl bromide ( BPB ) or photooxidation by Rose Bengal ( RB ) ; Tyr , by 2 - nitrobenzenesulphonyl fluoride ( NBSF ) ; and Trp , by o - nitrophenylsulphenyl chloride ( NPSC ) . - pubmed
The myotoxic and cytotoxic activities of BthTX - I , a Lys49 PLA(2 ) homologue , after modification by BPB , RB , NBSF and NPSC , were reduced to 50 % , 20 % , 75 % , 65 % and 13 % , 0.5 % , 76 % , 58 % , respectively . - pubmed
BPB - treated BthTX - II , an Asp49 PLA(2 ) homologue , lost most of its catalytic , indirect hemolytic , anticoagulant , myotoxic and cytotoxic activities . - pubmed
Lethality caused by BthTX - I and - II was strongly reduced after treatment with BPB or RB , but only partially with NBSF or NPSC . - pubmed
Serum creatinine ( SCr ) , TSH , FT4 and other chemicals were compared before and 2 mo after L - thyroxine ( LT4 ) substitution therapy . - pubmed
PATIENTS AND METHODS : Three groups of patients with at least one colorectal adenoma were included in a case control study : 401 heavy drinkers ( group HD , mean daily alcohol intake 117 ( SD 4 ) g / day for a mean duration of 22 ( SD 0.6 ) years ) , aged 57 ( 0.5 ) years ( 78 % men ) ; 152 patients suffering from irritable bowel syndrome ( IBS ) , aged 61 ( 0.9 ) years ( 57 % male ) ; and 108 patients with a family history ( FH ) of colorectal adenoma or cancer , aged 55 ( 1 ) years ( 64 % male ) . - pubmed
Cytomegalovirus UL97 phosphotransferase mutations that affect susceptibility to ganciclovir . - pubmed
Low drug levels resulting from oral GCV therapy may predispose the virus to the initial selection of these low - grade UL97 resistance mutations and to later accumulation of other mutations and greater resistance . - pubmed
For arsenic speciation a variety of mass spectrometric techniques , mainly inductively coupled plasma mass spectrometry ( ICP - MS ) and electrospray tandem mass spectrometry ( ES - MS / MS ) coupled on - line with high - performance liquid chromatography ( HPLC ) , are in use . - pubmed
Product ion scans with multiple stages of tandem MS ( MS(n ) ; n=2 - 4 ) were used to acquire diagnostic data for each arsenosugar . - pubmed
The coding sequence fused to the cleavable glycoprotein signal peptide gp 64 , and a C - terminal histidine tag was placed under the transcriptional control of the Orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate - early 2 ( OpIE2 ) promoter . - pubmed
Gender- and race - specific determination of albumin excretion rate using albumin - to - creatinine ratio in single , untimed urine specimens : the Coronary Artery Risk Development in Young Adults Study . - pubmed
Although albumin excretion rate is commonly estimated by using albumin / creatinine ratio ( A / C ) , gender and race differences in creatinine excretion may bias this estimate . - pubmed
There are several reports from in vitro studies indicating that vanadium increases enzyme activity in insulin signalling pathways ; however , these findings have not been duplicated in vivo . - pubmed
Insulin dependent GLUT4 is a major glucose transporter present in skeletal muscle , adipocytes and heart . - pubmed
However , with a single dose of BMOV the GLUT4 level was restored to normal . - pubmed
RESULTS : There was a decline of liver hydroxyline and serum AST and ALT in oxymatrine group compared to those of the D - GalN group . - pubmed
The SOD activity was ( 149.81 15.28 ) NU / mg in oxymatrine pretreatment group and ( 95.22 16.33 ) NU / mg in the model group ( t=7.309 , P<0.01 ) ; ( 157.68 19.54 ) NU / mg in the treatment group compared with ( 119.88 14.94 ) NU / mg in the model group ( t=4.348 , P<0.01 ) . - pubmed
faecalis strain V583 and here report the identification of a chromosomal gene , katA , encoding a heme - containing cytoplasmic catalase . - pubmed
A hexahistidyl - tagged version of the catalase was purified , and major characteristics of the enzyme were determined . - pubmed
It contains one protoheme IX group per KatA polypeptide . - pubmed
faecalis cells grown in the presence of heme in the medium ; about 2 and 10 micro M hemin was required for half - maximal and maximal production of catalase , respectively . - pubmed
BIII-890 - CL is a non - orally active sodium channel blocker under development by Boehringer Ingelheim Corp for the potential treatment of thromboembolic stroke . - pubmed
Pharmacological profile of MEN 11066 , a novel potent and selective aromatase inhibitor . - pubmed
No change in 11beta - hydroxylase or 21 - hydroxylase activities was produced in vitro by the addition of 10 microM MEN 11066 . - pubmed
These results demonstrated that MEN 11066 is a potent inhibitor of aromatase which does not interfere with the cytochrome P450 involved in the synthesis of other steroids or in the metabolism of xenobiotics . - pubmed
Down - regulation of basolateral ( Ntcp , Oatp1 , and Mrp3 ) and canalicular ( Mrp2 , Bsep ) transporter mRNA occurred after treatment with IL-6 , turpentine , and LPS . - pubmed
A novel methoxy group migration from the carbonyl group to the phosphoryl group was observed in ESI - MS2 . - pubmed
Biotin synthase is responsible for the synthesis of biotin from dethiobiotin and sulfur . - pubmed
Although the name of the protein implies that it functions as an enzyme , it has been consistently reported that biotin synthase produces & lt;1 molecule of biotin per molecule of protein in vitro . - pubmed
Functional COMT variant predicts response to high dose pyridoxine in Parkinson ' s disease . - pubmed
Pyridoxal-5 - phosphate , the biological active form of pyridoxine , is a cofactor for dopa - decarboxylase ( DDC ) enzyme . - pubmed
However , this effect can be negated in the presence of a DDC inhibitor , which potentiates plasma levodopa level . - pubmed
A single nucleotide polymorphism at the nucleotide 1947 in the catechol - O - methyltransferase ( COMT ) gene encodes the high ( COMT(H ) ) and low activity ( COMT ( L ) ) forms of the enzyme . - pubmed
Using a multivariate model , the presence of the COMT(L ) allele predicted response to pyridoxine , with the best outcome observed in COMT(L / L ) homozygotes . - pubmed
This study aims to estimate the tolerable lactose intake which can be utilized in the digestion by lactase and in the fermentation by intestinal microbes in Japanese female adults . - pubmed
A second lactose tolerance test involving 10 g and 30 g of lactose was carried out in 10 subjects showing resistance to diarrhea , and serum glucose and insulin levels and the amount of hydrogen excreted in the breath were measured for comparison with those of glucose and lactitol . - pubmed
Serum glucose and insulin levels were scarcely elevated following the ingestion of both 10 g and 30 g of lactose , while the amount of hydrogen excreted in the breath was greatly increased following the ingestion of 30 g of lactose , but these levels were less following the ingestion of 10 g of lactose . - pubmed
In contrast , the ingestion of 15 g of glucose significantly increased blood glucose and insulin levels , while no hydrogen was detected in the breath . - pubmed
The digestive capacity of lactase is less than 10 g of lactose by single ingestion , while intestinal microflora are able to ferment approximately 20 - 30 g of lactose . - pubmed
The ability of aluminum to inhibit the ( Na (+)/ K (+)) ATPase activity has been observed by several investigators . - pubmed
However , no studies have focused on determining the change in ( Na (+)/ K (+)) ATPase isoforms caused by chronic exposure to aluminum and its relation with aluminum toxicity . - pubmed
Aluminum accumulation upon chronic dietary AlCl ( 3 ) administration significantly decreased the ( Na (+)/ K (+)) ATPase activity measured in the presence of nonlimiting Mg - ATP concentrations , without compromising protein expression of alpha - subunit isoforms in brain and kidney . - pubmed
Aluminum - induced synaptosomal ( Na (+)/ K (+)) ATPase inhibition was due to a reduction in the activity of isozymes containing alpha ( 1 )- alpha ( 2 ) and alpha(3)-subunits . - pubmed
The onset of enzyme inhibition was accompanied by a decrease of the ( Na (+)/ K (+)) ATPase sensitivity to submicromolar concentrations of ouabain , and it preceded major damage in plasma membrane integrity and energy supply , as revealed by the analysis of lactate dehydrogenase leakage and endogenous adenine nucleotides . - pubmed
The data suggest that , during chronic dietary exposure to AlCl ( 3 ) , brain ( Na (+)/ K (+)) ATPase activity drops , even if no significant alterations of catalytic subunit protein expression , cellular energy depletion , and changes in cell membrane integrity are observed . - pubmed
PKA - dependent and PKA - independent pathways for cAMP - regulated exocytosis . - pubmed
Until recently , it was generally thought that the effects of cAMP in regulated exocytosis are mediated by activation of cAMP - dependent protein kinase ( PKA ) , a major cAMP target , followed by phosphorylation of the relevant proteins . - pubmed
Although the involvement of PKA - independent mechanisms has been suggested in cAMP - regulated exocytosis by pharmacological approaches , the molecular mechanisms are unknown . - pubmed
Newly discovered cAMP - GEF / Epac , which belongs to the cAMP - binding protein family , exhibits guanine nucleotide exchange factor activities and exerts diverse effects on cellular functions including hormone / transmitter secretion , cell adhesion , and intracellular Ca ( 2 + ) mobilization . - pubmed
cAMP - GEF / Epac mediates the PKA - independent effects on cAMP - regulated exocytosis . - pubmed
Thus cAMP regulates and modulates exocytosis by coordinating both PKA - dependent and PKA - independent mechanisms . - pubmed
The species of phosphatidylethanolamine ( PE ) and phosphatidylinositol ( PI ) were all detected as the [ M - H](- ) ions . - pubmed
A conformationally flexible , urea - based tripodal anion receptor : solid - state , solution , and theoretical studies . - pubmed
The receptors are based around a hexasubstituted aryl core and contain both urea and pyridinium functionalities . - pubmed
Ascorbate - mediated iron release from ferritin in the presence of alloxan . - pubmed
Release of iron from ferritin requires reduction of ferric to ferrous iron . - pubmed
We investigated the ability of ascorbate to catalyze the release of iron from ferritin in the presence of alloxan . - pubmed
Incubation of ferritin with ascorbate alone elicited iron release ( 33 nmol/10 min ) and the generation of ascorbate free radical , suggesting a direct role for ascorbate in iron reduction . - pubmed
Iron release by ascorbate significantly increased in the presence of alloxan , but alloxan alone was unable to release measurable amounts of iron from ferritin . - pubmed
Superoxide dismutase significantly inhibited ascorbate - mediated iron release in the presence of alloxan , whereas catalase did not . - pubmed
(- ) ) generated in reaction systems containing both ascorbate and alloxan decreased significantly upon addition of ferritin , suggesting that A. - pubmed
Although release of iron from ferritin and generation of A. - pubmed
(- ) is the main reductant involved in ascorbate - mediated iron release from ferritin in the presence of alloxan and that both dialuric acid and A. - pubmed
Olmesartan treatment resulted in a significant decrease of BP , urinary protein excretion , plasma glucose ( 99 + /- 16 versus 92 + /- 14 mg / dl ; P & lt ; 0.05 ) , insulin ( 23.1 + /- 8.8 versus 19.9 + /- 9 ; P & lt ; 0.05 ) , HOMA index ( 6.0 + /- 2.7 versus 4.7 + /- 2.8 ; P & lt ; 0.05 ) , and glycated hemoglobin ( 5.33 + /- 0.58 versus 4.85 + /- 0.81 % ; P & lt ; 0.01 ) . - pubmed
Oligonucleotides containing a methylene bridge between Nl or N9 of the heterocyclic base and C1 ' of the pentofuranosyl ring ( homo - N - oligonucleotides ) were synthesized . - pubmed
We altered amino acids in TRM6 and TRM61 that were predicted to be important for the stability of the heteroligomer based on this model . - pubmed
These findings , in conjunction with the therapeutic response of NES to sertraline , indicate that the serotonin system is involved in the pathophysiology of NES . - pubmed
Electrospray mass spectrometry showed that the modified enzyme preparation was a mixture of proteins containing from 2 to 5 covalently linked PEG chains per subtilisin molecule . - pubmed
PEG - subtilisin catalyzed transesterification between vinyl butyrate and 1 - hexanol was optimum at 0.55 MH ( 2 ) O , while hydrolysis prevailed above 2 MH ( 2 ) O. - pubmed
Subtilisin powder and celite - immobilized subtilisin were more stable and less susceptible to autolysis than the PEG - modified enzyme . - pubmed
Circular dichroism and intrinsic protein - fluorescence studies showed that the conformation of PEG - subtilisin did not change as a function of water concentrations between 0 and 9 M. The K(m , app ) value of PEG - subtilisin for 1 - hexanol was highly influenced by water , which behaved as a competitive inhibitor in the transesterification reaction with an affinity for the enzyme similar to that of the alcohol . - pubmed
Lyoprotectants such as sorbitol and free PEG did not influence the activity of PEG - subtilisin but notably increased the activity of subtilisin powder and celite - immobilized subtilisin . - pubmed
An expanded polyQ sequence in the huntingtin gene is known to cause the huntingtin protein to aggregate and form intracellular inclusions as disease progresses . - pubmed
This review focuses on key questions remaining for how the expanded polyQ sequences affect the aggregation properties of the huntingtin protein and the corresponding effects on cellular machinery . - pubmed
Kinetic analysis revealed that the reduction in the AUC of a substrate of CYP3A4 produced by consecutive administration of an inducer of CYP3A4 could be approximated by the equation 1/(1 + CRCYP3A4 * ICCYP3A4 ) , where CRCYP3A4 is the ratio of the apparent contribution of CYP3A4 to the oral clearance of a substrate and ICCYP3A4 is the apparent increase in clearance of a substrate produced by induction of CYP3A4 . - pubmed
The CRCYP3A4 was calculated for 22 substrates on the basis of the previously reported method from inhibitory DDI studies using a potent CYP3A4 inhibitor such as itraconazole or ketoconazole . - pubmed
In addition , rifampicin was found to be the most potent inducer among the compounds analysed in the present study , with an ICCYP3A4 value of 7.7 , followed by phenytoin and carbamazepine , with values of 4.7 and 3.0 , respectively . - pubmed
It was indicated that coadministration of rifampicin , phenytoin and carbamazepine may reduce plasma AUCs to less than half for a broad range of CYP3A4 substrate drugs , with CRCYP3A4 values greater than 0.13 , 0.21 and 0.33 , respectively . - pubmed
Reversal of P - glycoprotein - mediated multidrug resistance by the murine double minute 2 antagonist nutlin-3 . - pubmed
Nutlin-3 is a MDM2 inhibitor under preclinical investigation as nongenotoxic activator of the p53 pathway for cancer therapy . - pubmed
Effects of nutlin-3 single treatment were much more pronounced in p53 wild - type cell lines ( IC(50)s & lt;3 micromol / L ) than in p53 - mutated cell lines ( IC(50)s & gt;17 micromol / L ) . - pubmed
In sharp contrast to the expectations , nutlin-3 concentrations that did not affect viability of p53 - mutated cell lines strongly increased the efficacy of vincristine in p53 - mutated , P - glycoprotein ( P - gp)-overexpressing cell lines ( decrease in IC(50)s 92- to 3,434 - fold ) . - pubmed
Moreover , nutlin-3 reduced efflux of rhodamine 123 and other fluorescence dyes that are effluxed by P - gp . - pubmed
Kinetic studies and investigation of P - gp - ATPase activity showed that nutlin-3 is likely to act as a P - gp transport substrate . - pubmed
Examination of the nutlin-3 enantiomers nutlin-3a and nutlin-3b revealed that , in contrast to MDM2 - inhibitory activity that is limited to nutlin-3a , both enantiomers similarly interfere with P - gp - mediated drug efflux . - pubmed
In conclusion , nutlin-3 - induced inhibition of P - gp and MRP-1 was discovered as a novel anticancer mechanism of the substance in this report . - pubmed
Effect of the acidic strength on the vapor phase Beckmann rearrangement of cyclohexanone oxime over the MFI zeolite : an embedded ONIOM study . - pubmed
Within the lesion , Sox-2 expression increased transiently , while the number of nestin - positive ependymal cells increased with a concomitant enhancement of proliferation , as indicated by the mitotic markers Ki67 and bromo - deoxyuridine . - pubmed
The mutations responsible for the spontaneous resistance to streptomycin and spectinomycin were identified in the rpsL and rrs genes , respectively . - pubmed
Elimination of the Kv1.3 voltage - dependent potassium channel gene produces striking changes in the function of the olfactory bulb , raising the possibility that this channel also influences other sensory systems . - pubmed
In particular , no axonal staining of Kv1.3 was detected , and most prominent labeling was in structures surrounding the somata of the principal neurons , suggesting specific localization to the large calyx of Held presynaptic endings that envelop the principal cells . - pubmed
The presence of Kv1.3 in presynaptic terminals was confirmed by coimmunolocalization with the synaptic markers synaptophysin , syntaxin , and synaptotagmin and by immunogold electron microscopy . - pubmed
After GDC embolization , adjunctive therapies , such as ventricular and/or spinal drainage ( 67 % ) , intrathecal administration of urokinase ( 41 % ) , continuous cisternal irrigation ( 16 % ) , and external decompression ( 16 % ) , were performed . - pubmed
The total syntheses of tetrapeptides tubulysins D ( 1 b ) , U ( 1 c ) , and V ( 1 d ) , which are potent tubulin polymerization inhibitors , are described . - pubmed
Exogenous urocortin-2 increased the conditioned fear - induced expression of c - Fos in the central amygdala ( CeA ) , and parvocellular neurons of the paraventricular hypothalamic nucleus ( pPVN ) , and revealed the effect of conditioned fear in the medial amygdala ( MeA ) . - pubmed
Elevated levels of prolactin ( PRL ) , oxytocin , progesterone and glucocorticoids are characteristics of lactation , and the pronounced fluctuation of these hormones occurring in this phase may play a role protecting the hippocampus . - pubmed
Arg149 is involved in switching the low affinity , open state of the binding protein AfProX into its high affinity , closed state . - pubmed
The structure of a mutant AfProX protein ( substitution of Tyr111 with Ala ) in complex with GB was solved in the open liganded conformation to gain further insight into ligand binding . - pubmed
We found that a network of amino acid side chains communicates the presence of GB toward Arg149 , which increases ligand affinity and induces domain closure of AfProX. - pubmed
These results were corroborated by molecular dynamics studies and support the view that Arg149 finalizes the high - affinity state of the AfProX substrate binding protein . - pubmed
The neurorescuing effect of A68930 ( a potent selective D ( 1 ) agonist ) and its role on the regulation of hypothalamus - pituitary - adrenal ( HPA)-axis have been investigated . - pubmed
CUS caused a significant decrease in the number of TH ir neurons in the striatum , medial forebrain bundle , ventral tegmental area and substansia nigra and GR in the cortex , striatum and hippocampus as compared to the non - stress controls ( NS ) . - pubmed
AS enhanced the activities of superoxide dismutase ( SOD ) and glutathione peroxidase ( GSH - Px ) in the cortex and striatum , while CUS reduced the activities of SOD and catalase ( CAT ) in the cortex , striatum and hippocampus , when compared with NS . - pubmed
Increased GSH - Px activity , with reduced glutathione and increased lipid peroxidation was observed in both AS and CUS in selected brain regions as compared to NS . - pubmed
In conclusion , present findings suggest that the stress - induced immunoreactivity of TH and GR in distinct brain regions are modulated by A68930 leading to the normalization of HPA - axis response . - pubmed
A68930 also influenced the brain antioxidant machinery probably through the restoration of stress - induced changes in the dopaminergic system and its crosstalk with GR . - pubmed
Modified Moore swabs ( MMS ; consisting of a polyvinyl chloride cartridge filled with gauze ) capture microorganisms within the packed gauze as water flows through the cartridge , while continuous flow centrifugation ( CFC ) uses centrifugation to sediment the microorganisms while water continuously flows in the system . - pubmed
By incorporating polymer electrolyte into the carefully selected HP system , electrolyte layers / channels with length scales of a few tens of nanometers to micrometers can be formed with controlled orientation and anisotropy ; ionic conductivity anisotropy as high as 37 has been achieved . - pubmed
To evaluate the contribution of base stacking to the incorporation efficiency of dNTPs when a DNA polymerase encounters an abasic site , we varied the penultimate base pairs ( PBs ) adjacent to the abasic site using all 16 possible combinations . - pubmed
The efficiency of incorporation for incoming dNTPs opposite an abasic site varied between 2- and 210 - fold depending on the identity of the PB . - pubmed
Crystal structures of the ternary complexes show that the surface of the incoming base was stacked against the PB 's interface and that the kinetic parameters for dNMP incorporation were consistent with specific features of base stacking , such as surface area and partial charge - charge interactions between the incoming base and the PB . - pubmed
Without a templating nucleotide residue , an incoming dNTP has no base with which it can hydrogen bond and can not be desolvated , so that these surrounding water molecules become ordered and remain on the PB 's surface in the ternary complex . - pubmed
When these water molecules are on top of a hydrophobic patch on the PB , they destabilize the ternary complex , and the incorporation efficiency of incoming dNTPs is reduced . - pubmed
Vectors with dominant selective markers for G418 , hygromycin B , zeocin , and rho - fluoro - DL - phenylalanine were used to successfully transform JP1 cells . - pubmed
Promastigotes of Deltaatg4.2 but not Deltaatg4.1 were more susceptible than wild type promastigotes to starvation and oxidative stresses , which correlated with increased reactive oxygen species levels and oxidatively damaged proteins in the cells as well as impaired mitochondrial function . - pubmed
CONCLUSIONS : The results of this study suggest that bevacizumab is not promising for clinical implementation in the setting of HIPEC : the effects on tumour size were ambivalent and there was no statistically significant benefit in TGD . - pubmed
Vesicular monoamine transporter type 2 ( VMAT2 ) proteins in nerve terminals uptake dopamine into synaptic vesicles , preventing its cytoplasmic accumulation and toxic damage to nigral neurons . - pubmed
Presence of the JAK2 V617F mutation in a patient with chronic neutrophilic leukemia and effective response to interferon alpha-2b . - pubmed
We used a modified staining approach with a single MHC Class I tetramer ( K(d ) ) bound to two different fluorochromes to discriminate between the Class I - binding and fluorochrome - streptavidin - binding B cells with a high degree of specificity and binding efficiency . - pubmed
coli isobutanol tolerance through engineering its global transcription factor cAMP receptor protein ( CRP ) . - pubmed
coli isobutanol tolerance from its transcriptional level by engineering its global transcription factor cAMP receptor protein ( CRP ) . - pubmed
Random mutagenesis libraries were generated by error - prone PCR of crp , and the libraries were subjected to isobutanol stress for selection . - pubmed
Variant IB2 ( S179P , H199R ) was isolated and exhibited much better growth ( 0.18 h(-1 ) ) than the control ( 0.05 h(-1 ) ) in 1.2 % ( v / v ) isobutanol ( 9.6 g / L ) . - pubmed
Genome - wide DNA microarray analysis revealed that 58 and 308 genes in IB2 had differential expression ( & gt;2 - fold , p & lt ; 0.05 ) in the absence and presence of 1 % ( v / v ) isobutanol , respectively . - pubmed
When challenged with isobutanol , genes related to acid resistance ( gadABCE , hdeABD ) , nitrate reduction ( narUZYWV ) , flagella and fimbrial activity ( lfhA , yehB , ycgR , fimCDF ) , and sulfate reduction and transportation ( cysIJH , cysC , cysN ) were the major functional groups that were up - regulated , whereas most of the down - regulated genes were enzyme ( tnaA ) and transporters ( proVWX , manXYZ ) . - pubmed
As demonstrated by single - gene knockout experiments , gadX , nirB , rhaS , hdeB , and ybaS were found associated with strain isobutanol resistance . - pubmed
The intracellular reactive oxygen species ( ROS ) level in IB2 was only half of that of the control when facing stress , indicating that IB2 can withstand toxic isobutanol much better than the control . - pubmed
The normalized specific flux through the MBR and RO membranes was evaluated along with inorganic and organic constituents in the influent and effluent of each process . - pubmed
Increasing the SRT in the MBR led to an increase in the removal of bulk DOC , protein , and carbohydrates , as has been observed in previous studies . - pubmed
However , the opposite trend was observed for fouling of the RO membranes ; increasing the SRT of the MBR resulted in increased fouling of the RO membranes . - pubmed
These results indicate that the constituents that foul MBR membranes are not the same as those that foul RO membranes ; to be an RO membrane foulant in a MBR - RO system , the constituents must first pass through the MBR membranes without being retained . - pubmed
Gene deletion analyses confirmed that the enoyl - CoA hydratase / dehydrogenase Fox2p , the putative 3 - hydroxypropionyl - CoA hydrolase Ehd3p , the 3 - hydroxypropionate dehydrogenase Hpd1p , and the putative malonate semialdehyde dehydrogenase Ald6p essentially contribute to propionyl - CoA degradation and its conversion to acetyl - CoA. - pubmed
The function of Hpd1p was further supported by the detection of accumulating 3 - hydroxypropionate in the hpd1 mutant on propionyl - CoA - generating nutrients . - pubmed
Substrate specificity of Hpd1p was determined from recombinant purified enzyme , which revealed a preference for 3 - hydroxypropionate , although serine and 3 - hydroxyisobutyrate could also serve as substrates . - pubmed
Nutritional status was assessed from serum albumin , urea , creatinine , Hb , ferritin , and folate and plasma amino acid concentrations at 4 months . - pubmed
Intra - PVN administration of H3 ( 1.8 - 1620 pmol ) significantly increased plasma ACTH at 1620 pmol H3 and corticosterone at 180 - 1620 pmol H3 at 30 min following injection compared with vehicle . - pubmed
Treatment of hypothalamic explants with H3 ( 10 - 1000 nM ) stimulated the release of corticotrophin - releasing hormone ( CRH ) and arginine vasopressin ( AVP ) , but H3 had no effect on the release of ACTH from in vitro pituitary fragments . - pubmed
These results suggest that relaxin-3 may regulate the HPA axis , via hypothalamic CRH and AVP neurons . - pubmed
ISG activation depends on Tat interaction with MAP2K3 , MAP2K6 , and IRF7 promoters and a single exon Tat protein more strongly modulated the luciferase activity mediated by MAP2K3 , MAP2K6 , and IRF7 promoter sequences located 5 ' of the RNA start site than the wild type two - exon Tat , while a cysteine and lysine Tat mutants , reduced in LTR transactivation , had negligible effects on these promoters . - pubmed
Lithium treatment induced somatic growth retardation , renal microcysts and dilatations in cortical collecting duct ; it increased cortical cell proliferation and inactive pGSK-3beta abundance ; it lowered aquaporin-2 ( AQP2 ) protein abundance and induced polyuria with decreased ability to concentrate the urine ; and it increased COX-2 protein level in thick ascending limb . - pubmed
Concomitant treatment with lithium and a specific COX-2 inhibitor , parecoxib ( 5 mg / kg per day , P10 - P34 ) , did not prevent lithium - induced microcysts and polyuria , but improved urine concentrating ability transiently after a 1 - desamino-8 - D - arginine vasopressin challenge . - pubmed
COX-2 inhibition did not reduce cortical lithium - induced cell proliferation and phosphorylation of glycogen synthase kinase-3beta ( GSK-3beta ) . - pubmed
Discovery of ( S )- 1 -( 1 -( Imidazo [ 1,2 - a ] pyridin-6 - yl ) ethyl )- 6 -( 1 - methyl-1H - pyrazol-4 - yl )- 1H -[ 1,2,3 ] triazolo [ 4,5 - b ] pyrazine ( volitinib ) as a highly potent and selective mesenchymal - epithelial transition factor ( c - Met ) inhibitor in clinical development for treatment of cancer . - pubmed
Different non - ionic cellulose ethers ( methyl cellulose , MC ; hydroxyethyl cellulose , HEC ; hydroxypropyl cellulose , HPC ; hydroxypropylmethyl cellulose , HPMC ) and microcrystalline cellulose ( MCC ) were investigated as matrix formers for preparation of mini - tablets targeting vaginal drug delivery . - pubmed
Hexyl aminolevulinat hydrochloridum ( HAL ) was used as a model drug . - pubmed
PSi layers were synthesized using a two - electrode Teflon electrochemical cell ; the etching solution contained hydrogen peroxide 30 % , hydrofluoric acid 40 % ( HF ) , and methanol . - pubmed
These Si - ncs colloidal solutions , measured by photoluminescence ( PL ) , revealed efficient blue - green or violet emission intensities . - pubmed
After the experimental periods , blood samples were collected for measurement of oestradiol , calcium , phosphorus and alkaline phosphatase . - pubmed
On day 7 , alkaline phosphatase activity , calcium and phosphorus were higher in the OVX - RLX group than in the OVX - veh group ( P & lt ; 0.001 ) , but immunolabelling for RANKL and HIF-1alpha was lower in OVX - RLX group ( P & lt ; 0.001 ) . - pubmed
Mitochondrial iron accumulation exacerbates hepatic toxicity caused by hepatitis C virus core protein . - pubmed
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT : Elevated rates of substance use disorders ( SUDs ) , particularly ( but not only ) alcohol use , are observed among post - weight loss surgery ( WLS ) patients . - pubmed
GlyR function is affected by a variety of allosteric modulators including drugs of abuse , such as ethanol and inhalants and the ubiquitous divalent cation zinc . - pubmed
A direct comparison of agonist concentration - response curves showed that zinc enhancement was greater , overall , for taurine - activated than glycine - activated receptors . - pubmed
In addition , zinc only enhanced taurine- but not glycine - activated GlyR when agonists were applied at saturating concentrations . - pubmed
These data suggest that zinc affects taurine affinity , as well as the probability of channel opening at sub - maximal taurine concentrations , and that the magnitude of allosteric modulation at the GlyR depends on the efficacy of the agonist tested . - pubmed
Cloning , purification , crystallization and preliminary X - ray studies of a carbohydrate - binding module from family 64 ( StX ) . - pubmed
In this context , the study of carbohydrate - binding modules ( CBMs ) , which are involved in guiding the catalytic domains of glycoside hydrolases to polysaccharides , is crucial for enzyme development . - pubmed
We recently showed using the beta - arrestin - biased beta - blocker , carvedilol that beta - arrestin1 - biased beta1 - adrenergic receptor cardioprotective signalling stimulates the processing of miR-150 in the heart . - pubmed
Supported lipid bilayers on glass coverslips that contain phosphatidylserine and phosphatidylinositol 4,5 - bisphosphate ( PI ( 4,5 ) P2 ) make actin bundles termed filopodia - like structures that contain fascin and have vasodilator - stimulated phosphoprotein ( VASP ) at their growing tips , indicating that these resemble filopodia growing from the plasma membrane . - pubmed
The combination of PI ( 4,5 ) P2 and phosphatidylinositol 3 - phosphate in curved liposomes or supported bilayers on glass nanospheres uses Snx9 , Cdc42 , N - WASP ( neuronal - Wiskott - Aldrich syndrome protein ) , and Arp2 / 3 complex for actin polymerization suggesting that this membrane may mimic the progression from plasma membrane to endosomes . - pubmed
Here we describe how to prepare high - speed supernatant frog egg extracts and phosphoinositide - containing liposomes and supported lipid bilayers that can assemble actin structures . - pubmed
The chemical structures of P1 A was identified by complete acid hydrolysis , partial acid hydrolysis , periodate oxidation - Smith degradation , methylation analysis , IR and NMR . - pubmed
The results showed that P1 A had a backbone consisting rhamnose , mannose , glucose and galactose . - pubmed
Fatty acid ( FA ) -sensitive neurons are present in the brain , especially the hypothalamus , and play a key role in the neural control of energy and glucose homeostasis including feeding behavior , secretion insulin and action . - pubmed
Molecular effectors of these FA effects include ion channels such as chloride , potassium or calcium . - pubmed
Developmental exposure to bisphenol A alters expression and DNA methylation of Fkbp5 , an important regulator of the stress response . - pubmed
The estrogen receptor ( ER ) antagonist ICI 182,780 or ERbeta knock - down affected Fkbp5 expression and methylation similarly to BPA . - pubmed
Further , BPA 's effect on Fkbp5 was abolished upon knock - down of ERbeta , suggesting a role for this receptor in mediating BPA 's effects on Fkbp5 . - pubmed
Decreasing in retrogradation was observed in both NRF and GRF with MPP added of all levels . - pubmed
The hydrogen bonds and hydrophilic interactions between phenolic compounds with amylopectin molecule may be involved the decrease of starch retrogradation , especially GRF . - pubmed
Assessment of 25 CYP2D6 alleles found in the Chinese population on propafenone metabolism in vitro . - pubmed
The aim of this study was to assess the catalytic characteristics of 25 alleles ( CYP2D6.1 and 24 CYP2D6 variants ) and their effects on the metabolism of propafenone in vitro . - pubmed
The levels of SC and other laboratory indexes including complete blood counts , leukocytes , the immature - to - total - neutrophil ratio , procalcitonin ( PCT ) , C - reactive protein , and blood lactate were detected , cytokines [ interleukin ( IL )- 2 , IL-6 , and interferon - gamma ] released by neutrophils were also determined . - pubmed
Moreover , SAF plants exhibited enhanced drought stress tolerance and better growth under drought stress treatment , which was accompanied by a reduced transpiration rate and lower reactive oxygen species contents . - pubmed
Randomized Trial of the Effect of Magnesium Sulfate Continuous Infusion on IL-6 and CRP Serum Levels Following Abdominal Aortic Aneurysm Surgery . - pubmed
However , IL-6 level was significantly less in intervention group early after the end of MgSO4 infusion comparing with control group ( P = 0.01 ) . - pubmed
According to the results of this study , continuous infusion of MgSO4 after AAA surgery may provide IL-6 suppression . - pubmed
DNA - mediated assembly of core - satellite structures composed of Zr ( IV )-based porphyrinic metal - organic framework ( MOF ) and NaYF4 , Yb , Er upconverting nanoparticles ( UCNPs ) for photodynamic therapy ( PDT ) is reported . - pubmed
Under NIR irradiation , visible light emitted from the UCNPs is absorbed by the core MOF NP to produce 1 O2 at significantly greater amounts than what can be produced from simply mixing UCNPs and MOF NPs . - pubmed
Sugars with heteroatoms other than oxygen have attained considerable importance in glycobiology and in drug design since they are often more stable in blood plasma due to their resistance to enzymes , such as glycosidases , phosphorylases and glycosyltransferases . - pubmed
MTT assay showed that si - NEAT1 remarkably inhibited the cell proliferation in three HCC cell lines . - pubmed
In this Account , we extend the GPF - EP method to describe bonds involving N electrons in M orbitals ( N & lt ; M ) and show its application to ( 2c1e ) bonds . - pubmed
The method is employed to generate new characteristics of the B11u(51P1 ) electronic state of Cd2 based on LIF excitation spectrum previously recorded using the B11u X10g+ ( 51S0 ) transition . - pubmed
Correctness of the method is additionally tested on artificially generated LIF excitation spectra based on well characterized b30u +( 53P1 ) X10g+ transition in Cd2 . - pubmed
INTRODUCTION : To compare the percentage of arterial oxygen saturation ( SpO2 ) in healthy teeth with confirmed pulp vitality between individuals with sickle cell anemia ( HbSS ) and normal hemoglobin A ( HbAA ) . - pubmed
Furthermore expression of the enzyme responsible for PGD2 synthesis ( hematopoietic prostaglandin D synthase , HPGDS ) was increased in obese adipose tissues and remained high after weight loss . - pubmed
Perioperatively , Levobupivacaine hydrochloride ( 15 mg ) was administered in the subarachnoid space , at the L4 - L5 lumbar intervertebral space , using a 29 Gauge needle . - pubmed
The results showed that long - term exposure to 1 mg / L CeO2 NPs induced the deterioration of denitrifying process , which was consistent with the inhibition of enzyme activities of nitrite oxidoreductase and nitrate reductase under CeO2 NPs . - pubmed
To test the efficacy of nicotine patch preloading for engaging proposed treatment targets during the pre - quit phases , we used multilevel models to compare post - smoking changes in symptoms and cravings during the preloading phases to post - smoking changes reported during the ad lib smoking phase . - pubmed
RESULTS : There was no significant difference in quit rates across the two conditions on the primary outcome of seven - day point prevalence smoking abstinence bioverified with breath carbon monoxide at six weeks post - quit date . - pubmed
We show that ANANAS tethering of cetuximab promotes a more efficient EGFR - dependent vesicle - mediated internalization . - pubmed
Broadband dielectric relaxation spectroscopy ( DRS ) has been used to investigate aqueous solutions of MgCl2 and CaCl2 up to concentrations of about 1.8 mol L-1 at 25 C over the frequency range 0.07 & lt;= nu / GHz & lt;= 89 . - pubmed
Detailed analysis of the dominant solvent mode centered at ~20 GHz showed that both Mg2 + and Ca2 + are strongly solvated , each immobilizing ~20 water molecules on the DRS timescale . - pubmed
Thus , resveratrol as a hypoxia - inducible factor-1 - alpha inhibitor increased the radiosensitization of breast cancer spheroid cells . - pubmed
4 - Nonylphenol - enhanced EZH2 and RNF2 expression , H3K27me3 and H2AK119ub1 marks resulting in silencing of p21CDKN1A in vitro . - pubmed
The phospho - CREB , SP1 and E2F-1 are enriched at proximal promoter of RNF2 and EZH2 and cyclin D1 , but not p21 . - pubmed
In 2018 , The Food and Drug Administration ( FDA ) of the US approved several novel agents for AML treatment as follows : ivosidenib , an inhibitor of isocitrate dehydrogenase-1 ; venetoclax , a potent inhibitor of bcl2 ; and glasdegib , an inhibitor of hedgehog signaling pathway . - pubmed
Moreover , clinical trials of alvocidib ( flavopiridol ) , an inhibitor of the CDK9 , pevonedistat , an inhibitor of NEDD8 , and APR-246 , a reactivator of mutant p53 , are in progress . - pubmed
Multidisciplinary care by an NST leads to fewer complications including infection and electrolyte disturbances , and better survival for patients receiving short- and long - term PN . - pubmed
Regarding infection , resorbable presented an absolute risk ( AR ) = 0.032 , and titanium an AR = 0.025 ( P = 1.0 ) . - pubmed
For soft tissue reaction , an AR = 0.120 was shown for resorbable , and an AR = 0.132 for titanium ( P = 0.85 ) . - pubmed
Removal of fixation showed an AR = 0.024 for resorbable , and an AR = 0.025 for titanium ( P = 1.0 ) . - pubmed
In this study , we demonstrated that glycol chitosan ( GC ) and oxidized hyaluronate ( OHA ) can be used to form a self - healing ferrogel in the presence of superparamagnetic iron oxide nanoparticles ( SPIONs ) without additional chemical cross - linkers . - pubmed
The overall characteristics of GC / OHA / SPION ferrogel varied based on the GC / OHA ratio , SPION content , and total polymer concentration . - pubmed
Interestingly , GC / OHA / SPION ferrogel was used to fabricate 3D - printed constructs of various shapes via an extrusion printing method . - pubmed
Experience with Teaching Different Modeling Techniques on the example of Glucose Insulin Regulation Model . - pubmed
Hands - on implementation using all these techniques and comparing them towards same system ( in our case glucose - insulin regulation ) we teach pros and cons of each technique . - pubmed
Fingolimod induces sequestration of lymphocytes in lymphoid organs by altering lymphocyte expression of sphingosine-1 - phosphate receptors . - pubmed
Major depressive disorder ( MDD ) is associated with alterations of GABAergic interneurons , notably somatostatin ( Sst ) as well as parvalbumin ( Pvalb ) , in cortical brain areas . - pubmed
Chemogenetic inhibition of all GABA interneurons ( Gad1 + ) , as well as Sst+ and Pvalb+ subtypes in the mPFC produced dose and time - dependent antidepressant effects in the forced swim and novelty suppressed feeding tests , and increased synaptic plasticity . - pubmed
In contrast , stimulation of Gad1 , Sst , or Pvalb interneurons in mPFC abolished the effects of scopolamine and prevented scopolamine induction of synaptic plasticity . - pubmed
An immunofluorescence microscopy analysis was used to investigate cellular oxidative stress and DNA damage using DCFDA fluorescence staining and an anti - gammaH2AX antibody . - pubmed
Full - dimensional quantum dynamics study of isotope effects for the H2 + NH2 / ND2 / NHD and H2 / D2 / HD + NH2 reactions . - pubmed
After elution from the antibody column at pH 11 the normal beta component , by dialysis against pyridoxal phosphate at pH 7.5 , could be restored to the enzymatically - active beta2 dimer with a specific activity of 1700 enzyme units / mg protein . - pubmed
[ The effect of chloramphenicol on in vitro colony forming cell ( CFC ) ( author 's transl ) ] . - pubmed
Glutamate responses showed similarities to those of the kainate / quisqualate receptor subtype . - pubmed
The reflex tracheal dilatations were abolished by the combination of intra - arterial propranolol ( 100 micrograms ) and transections of both the bilateral superior laryngeal nerves and the spinal cord at the C1 level . - pubmed
Insulin neither affected basal lipolysis nor inhibited dibutyl cAMP - stimulated lipolysis . - pubmed
Immunoprecipitation studies showed that all four IgG isotypes appear to recognize a similar set of antigens . - pubmed
Cytoplasmic tail deletion converts membrane immunoglobulin to a phosphatidylinositol - linked form lacking signaling and efficient antigen internalization functions . - pubmed
Transfected constructs lacking the normal three - amino acid cytoplasmic tail are expressed exclusively as phosphatidylinositol - linked proteins , lack both antigen presentation and signal transduction functions , and are internalized slowly following anti - Ig binding . - pubmed
The molecular mass of the cytoplasmic tail - deleted phosphatidylinositol - linked Ig molecule is consistent with cleavage of the transmembrane residues during processing . - pubmed
The sugar - binding sites of LOLI are occupied by four well - ordered water molecules each . - pubmed
Fluorescein isothiocyanate specifically modifies lysine 338 of cytochrome P-450scc and inhibits adrenodoxin binding . - pubmed
Treatment of cytochrome P-450scc with fluorescein isothiocyanate ( FITC ) resulted in covalent labeling with 1.0 + /- 0.1 eq of FITC . - pubmed
The modification of lysine 338 with FITC resulted in 85 + /- 15 % inhibition of adrenodoxin binding to cytochrome P-450scc . - pubmed
In a complementary experiment it was found that if a complex between adrenodoxin and native cytochrome P-450scc was formed in the presence of cholesterol and then treated with FITC , there was almost no labeling of lysine 338 . - pubmed
These experiments suggest that lysine 338 is located at the binding site for adrenodoxin and electrostatically interacts with one of the carboxylate groups on adrenodoxin that has been implicated in binding . - pubmed
The fluorescence emission of the FITC label on cytochrome P-450scc was only 14 % as large as that of an equivalent concentration of FITC - labeled bovine serum albumin , suggesting that it was quenched by Forster energy transfer to the heme group . - pubmed
The affinity of the binding sites for Ins ( 1,4,5 ) P3 was not influenced by the non - hydrolysable GTP analogues GTP gamma S and Gpp [ NH ] p or by preincubation of the binding protein with a preparation of partially purified protein kinase C in the presence of ATP and TPA ( 12 - O - tetradecanoylphorbol 13 - acetate ) . - pubmed
The variant could not be resolved from Hb A on cellulose acetate ( pH 8.4 ) , but isoelectric focusing showed a double band in the region of Hb A that is probably the variant and Hb A. However , the variant chain was clearly evident by globin chain analyses in acid and alkaline buffers . - pubmed
Chronic lithium prevets reserpine - induced supersensitivity of adeylate cyclase . - pubmed
We describe a synthetic oligonucleotide that can prime specifically reverse transcription of VSG mRNA out of total trypanosome poly(A)+ RNA . - pubmed
Our medium was a modification of that of Doyle and Roman with the addition of filter - sterilized FBP ( 0.2 % ferrous sulfate , 0.025 % sodium metabisulfite , 0.05 % sodium pyruvate ) , 0.1 % sodium lauryl sulfate , and 0.075 % agar . - pubmed
Thus the total cellular concentrations of both estradiol and progesterone receptors are lowest in the late secretory phase . - pubmed
In anovulatory cycles the concentrations of estradiol and progesterone receptors are high , similar to those of the late proliferative phase . " - pubmed
The dextran - coated charcoal assay method of McGuire and DeLaGarza is a well - known method for quantitation of oestrogen receptors in breast tumour tissues . - pubmed
The UV spectrum and TSP / LC / MS / MS daughter ion analysis led to the proposition of a bisamide structure for the major metabolite . - pubmed
TSP / LC / MS indicated that the molecular weight of the minor metabolite was 16 u higher than the bisamide , suggesting an oxidized analogue . - pubmed
Fluorescent choleretic and cholestatic bile salts take different paths across the hepatocyte : transcytosis of glycolithocholate leads to an extensive redistribution of annexin II . - pubmed
Treatment of couplets with glycine derivatives of lithocholate and ursodeoxycholate , but not cholate or chenodeoxycholate , led to a marked relocalization of annexin II , which initially became concentrated at the basolateral membrane , then moved to a perinuclear distribution and finally to the apical membrane as the incubation progressed . - pubmed
This suggests that lithocholate and ursodeoxycholate treatment leads to a rapid induction of transcytosis and that annexin II exchange occurs upon membrane fusion at all stages of the hepatocyte transcytotic pathway . - pubmed
coli strain CA using three independent methods : SDS gel electrophoresis to quantify the synthesized luciferase protein , EIA to quantify the enzyme native conformer and activity measurements . - pubmed
The increase in the enzyme activity correlated well with the increase in the ATP content in the cell . - pubmed
Inhibition of cytokine - primed eosinophil chemotaxis by nedocromil sodium . - pubmed
These cytokines have the capacity to modulate chemotactic responses of eosinophils toward platelet - activating factor , formyl - methionyl - leucyl - phenylalanine , ( FMLP ) and neutrophil - activating factor ( NAF)/IL-8 , but not toward complement fragment C5a ( C5a ) . - pubmed
CONCLUSIONS : The chemotactic responses toward C5a were inhibited by nedocromil sodium at higher concentrations than were required in the priming studies ( IC50 approximately 10 to 100 nmol / L ) . - pubmed
The GABAB receptor agonist , baclofen , directly hyperpolarized or produced an outward current ( single electrode voltage - clamp ) in all 66 neurones tested . - pubmed
2 - OH - saclofen , a selective GABAB receptor antagonist , competitively antagonized baclofen - induced hyperpolarizations ( n = 4 ) with equilibrium dissociation constants estimated in two neurones to be 6 and 23 microM. - pubmed
These studies demonstrate that baclofen acting on GABAB receptors increases potassium conductance in all lateral and ventrolateral periaqueductal gray neurones . - pubmed
Melatonin and adrenocorticotropic hormone levels in video display unit workers during work and leisure . - pubmed
The study investigated possible effects of VDU work on circulating melatonin and adrenocorticotropic hormone ( ACTH ) levels . - pubmed
Circulating melatonin levels decreased significantly during VDU work , whereas ACTH levels increased significantly . - pubmed
Mental strain during work was significantly and positively associated with circulating levels of ACTH but not melatonin . - pubmed
Mental strain might explain changes in ACTH levels , but specific factors that might contribute to changes in melatonin levels are unknown . - pubmed
Lines were divergently selected for 4 - wk BW ( HW and LW ) or plasma TPP ( HP and LP ) . - pubmed
The first 5 amino acids of the carboxyl terminus of phosphatidylinositol transfer protein ( PITP ) alpha play a critical role in inositol lipid signaling . - pubmed
PITP has a single lipid - binding site that can reversibly bind phosphatidylinositol ( PI ) and phosphatidylcholine ( PC ) and transfer these lipids between membrane compartments in vitro . - pubmed
The role of the carboxyl terminus was examined by comparing wild - type PITPalpha with PITPalpha in which 5 , 10 , and 20 amino acids were deleted from the C terminus . - pubmed
We conclude that ( a ) the carboxyl terminus of PITP plays a critical role in phospholipase C signaling ; ( b ) the transfer activity is not the only determining factor that dictates the restorative function of PITP in inositol lipid signaling ; and ( c ) the dominant inhibitory effects of Delta5- and Delta10 - PITP on wild - type PITP in phospholipase C signaling suggest the existence of a receptor for PITP . - pubmed
PACAP release from islets was bioassayed using the response of cytosolic Ca2 + concentration ( [ Ca2+]i ) in single beta - cells , monitored by dual - wavelength fura-2 microfluorometry . - pubmed
High glucose - stimulated insulin release from isolated islets was attenuated by PACAP antiserum but not by non - immune sera . - pubmed
The islet incubation medium with high glucose ( Med ) possessed a capacity , which was neutralized by PACAP antiserum , to increase [ Ca2 +]i in beta - cells . - pubmed
PACAP antiserum also neutralized the [ Ca2 +]i - increasing action of synthetic PACAP-38 and PACAP-27 , but not that of vasoactive intestinal polypeptide ( VIP ) and glucagon . - pubmed
Both Med and synthetic PACAP increased [ Ca2 +]i in beta - cells only in the presence of stimulatory , but not basal , glucose concentrations . - pubmed
[ Ca2 +] i measurements combined with immunocytochemistry with insulin antiserum revealed a substantial population of glucose - unresponsive beta - cells , many of which were recruited by PACAP-38 into [ Ca2 +]i responses . - pubmed
These results indicate that PACAP-38 is a novel islet substance that is synthesized and released by islet cells and then , in an autocrine and/or paracrine manner , potentiates and arouses beta - cell responses to glucose , thereby amplifying glucose - induced insulin secretion in islets . - pubmed
The authors measured total T cells , T - helper cells , T - suppressor cells , natural killer cells , and the synthesis of interleukin-1 , interleukin-6 , and tumor necrosis factor by peripheral blood mononuclear cells before and on Day 40 of fish oil supplementation . - pubmed
AMs from animals inhaling asbestos showed significant elevations ( p & lt ; .05 ) in nitrite / nitrate levels which were ameliorated by NG - monomethyl - L - arginine ( NMMA ) , an inhibitor of inducible nitric oxide synthase ( iNOS ) activity . - pubmed
Regulation of phospholipase C delta 1 activity by GTP - binding proteins : rhoA as an inhibitory modulator . - pubmed
Clinical implications of the types of cryoglobulins determined by two - dimensional polyacrylamide gel electrophoresis . - pubmed
Examining the clinical findings in the mono group ( those with monoclonal IgM alone ) and the poly group ( those with polyclonal IgM alone or polyclonal and monoclonal IgM ) we found clearly significant differences : more severe liver involvement in the poly group , and higher cryocrit and creatinine values , lower C4 level and more severe purpura in the mono group . - pubmed
SO ( 10 ) operator analysis for nu micro nu tau oscillations . - pubmed
Constitutive degradation of PML / RARalpha through the proteasome pathway mediates retinoic acid resistance . - pubmed
Treatment with retinoic acid ( RA ) induces degradation of PML / RARalpha , differentiation of leukaemic blasts , and disease remission . - pubmed
The steady - state , dinitrostilbene - disulfonate - sensitive current ( DeltaI ) was Na + and HCO3 - dependent and therefore was taken as flux through the cotransporter . - pubmed
Our results identify Eph receptors and ephrins as the first molecules known to demarcate individual lobules during development . - pubmed
Membrane topology of the amino - terminal region of the sulfonylurea receptor . - pubmed
The sulfonylurea receptor ( SUR ) is a member of the ATP - binding cassette family that is associated with Kir 6.x to form ATP - sensitive potassium channels . - pubmed
SUR is involved in nucleotide regulation of the channel and is the site of pharmacological interaction with sulfonylurea drugs and potassium channel openers . - pubmed
SUR contains three hydrophobic domains , TM(0 ) , TM(1 ) , and TM(2 ) , with nucleotide binding folds following TM(1 ) and TM(2 ) . - pubmed
We analyzed the topology of the amino - terminal TM(0 ) region of SUR1 using glycosylation and protease protection studies . - pubmed
Deglycosylation using peptide - N - glycosidase F and site - directed mutagenesis established that Asn(10 ) , near the amino terminus , and Asn(1050 ) are the only sites of N - linked glycosylation , thus placing these sites on the extracellular side of the membrane . - pubmed
Restoration by glycerol of p53 - dependent apoptosis in cells bearing the mutant p53 gene . - pubmed
CONCLUSIONS : Glycerol appears to function as a chemical chaperone that restores mp53 to wtp53 function . - pubmed
A precise single - point mutation was introduced into genes encoding acetohydroxyacid synthase ( AHAS ) , at a position known to confer imidazolinone resistance . - pubmed
It has been observed that heparin acts as a specific blocker of IP3 receptors and inhibits IP3 - mediated calcium release in various cell types , including vascular smooth muscle and airway smooth muscle . - pubmed
The cationic peroxidases , such as major basic protein and eosinophil peroxidase , are neutralized by the highly anionic heparin ; thus heparin inhibits the epithelial damage induced by some of these cationic proteins . - pubmed
The mGluR subtypes that are coupled to the hydrolysis of phosphoinositide contribute to both synaptic plasticity and glutamate - mediated excitotoxicity in neurons . - pubmed
Protein oxidation was estimated by electron spin resonance spectroscopy , after labeling of the ' peroxidized ' mitochondria with 4 - maleimido-2 , 2,6,6 - tetramethylpiperidine-1 - oxyl . - pubmed
We found that ( i ) low concentrations of flupirtine ( 10 microM ) protect lipids and also proteins ( with lesser efficiency ) from attacks of reactive oxygen species ; ( ii ) flupirtine remarkably delayed the decline of complex mitochondrial functions , such as the respiratory control or the Ca ( 2 + ) retention capacity of mitochondria , under oxidative stress ; and ( iii ) the ADP / ATP antiporter ( ANT ) , a main component of the oxidative phosphorylation machinery as well as a core component of the permeability transition pore complex , seems to be a membrane protein particularly protected by flupirtine . - pubmed
In conclusion , the preservation of the Ca ( 2 + ) buffer capacity of mitochondria and of the ANT activity against oxidative stress supports an antiapoptotic application of flupirtine . - pubmed
Complex 6.CH ( 2 ) Cl ( 2 ) crystallizes in the triclinic space group P1 with , at -158 degrees C , a = 13.4635(3 ) , b = 13.5162(3 ) , c = 23.2609(5 ) A , alpha = 84.9796(6 ) , beta = 89.0063(8 ) , gamma = 86.2375(6 ) degrees , Z = 2 , and V = 4207.3(3 ) A(3 ) . - pubmed
Glutamate is a key transmitter of vagal afferents acting partly on NMDA receptors . - pubmed
GABA in the dialysate was determined by high - performance liquid chromatography system and serum LH concentration was determined by radioimmunoassay . - pubmed
Conversely , in the OLN group , elevated CIC and diminished CH50 and C3 serum levels were significantly different ( P & lt ; 0.01 ) . - pubmed
The membrane - associated EGF receptors undergo rapid tyrosine phosphorylation and internalization upon ligand binding in ovarian cells , including luteal cells . - pubmed
Raf-1 and B - Raf , but not A - Raf , were activated by EGF ( 10 ng / ml ) and the pharmacological protein kinase C ( PKC ) activator phorbol myristate acetate ( PMA , 20 nM ) . - pubmed
Additionally , activated ERK was found in the nucleus of the cells following treatment with EGF ( 10 ng / ml ) and PMA ( PMA , 20 nM ) for 5 min . - pubmed
Depletion of PKC by chronic PMA treatment ( 2.5 microM , 24 h ) only partially inhibited the stimulatory effects of EGF on Raf-1 , ERK2 and ERK1 . - pubmed
Treatment with the MEK1 inhibitor PD098059 ( 50 microM ) abolished EGF - induced ERK activation . - pubmed
However , blocking EGF - induced ERK activation by pretreatment with PD098059 only partially attenuated EGF - induced c - fos and c - jun mRNA expression . - pubmed
Structural basis for a non - phosphorus - containing cyclic peptide binding to Grb2 - SH2 domain with high affinity . - pubmed
Blocking the interaction between phosphotyrosine ( pTyr)-containing activated receptors and the Src homology 2 ( SH2 ) domain of the growth factor receptor bound protein 2 ( Grb2 ) is considered to be an effective and non - cytotoxic strategy to develop new anti - proliferative agents due to its potential to shut down the Ras activation pathway . - pubmed
To understand the structural basis for the high - affinity binding of these novel non - phosphorus - containing inhibitors to the Grb2 - SH2 domain , we extensively studied herein the unique functional requirements of the acidic side chain in Tyr-2 position due to the absence of the phosphate group in these non - phosphorylated peptides . - pubmed
With both the peptidomimetic optimization of the amino acid side - chains and the constraint of the backbone conformation guided by molecular modeling , we developed several potent antagonists with low nanomolar range binding affinity , such as cyclic peptide 20 with an IC(50)=0.026 microM , which is one of the most potent non - phosphorus - containing Grb2 - SH2 antagonists reported to date . - pubmed
Adenovirus type 2 ( Ad2 ) early region 4 ORF4 ( E4orf4 ) triggers a major death pathway that requires its accumulation in cellular membranes and its tyrosine phosphorylation . - pubmed
Here we provide strong evidence that a physical interaction requiring the kinase domain of Src and the arginine - rich motif of E4orf4 is involved . - pubmed
METHODS : Baseline PANSS data from six double - blind , randomized registration trials of olanzapine , three in schizophrenia and three in acute bipolar mania , were used in these post - hoc analyses . - pubmed
Ciclesonide as a novel corticosteroid itself is inactive and used as prodrug form and needs to be cleaved by pulmonary esterases to acquire anti - inflammatory activity . - pubmed
We use laser flash photolysis and time - resolved Raman spectroscopy of CO - bound H93 G myoglobin ( Mb ) mutants to study the influence of the proximal ligand on the CO rebinding kinetics . - pubmed
In H93 G mutants , where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands ( e.g. - pubmed
Unlike topical corticosteroid agents , tacrolimus ointment does not cause a reduction in collagen synthesis or skin thickness . - pubmed
Because tacrolimus ointment does not cause skin atrophy , it may be safely used for months or years on all skin areas , including the face and intertriginous areas . - pubmed
Several specific proteins , including amino acid synthesis enzymes and vacuolar enzymes , are increased during nitrogen starvation in wild - type cells but not in autophagy - defective delta atg7 cells despite similar mRNA levels . - pubmed
Bulk protein synthesis was substantially reduced in delta atg7 cells under nitrogen starvation compared with wild - type cells . - pubmed
The total intracellular amino acid pool was reduced in delta atg7 cells , and the levels of several amino acids fell below critical values . - pubmed
Compared with the RDR , the sensitivity and specificity to detect vitamin A deficiency for each test was , respectively : serum retinol , 90 % and 78 % ; retinol - binding protein ( RBP ) , 40 % and 91 % ; retinol / RBP molar ratio , 60 % and 74 % ; conjunctival impression cytology , 44 % and 48 % ; slit - lamp examination , 20 % and 66 % ; tear film break - up time , 40 % and 69 % ; and Schirmer 's test , 20 % and 78 % . - pubmed
Hyphal uptake of ( 32 ) Pi and expression of plant Pi transporter genes were investigated in the rmc mutant and its wild - type progenitor ( 76R ) associated with three AMF . - pubmed
In all cases , the main fragmentation ( MS2 mode ) of the pseudomolecular ion leads to the neutral loss of a glutamyl moiety ( -129 Da ) , affording an ion that gives structural information upon an additional fragmentation ( MS3 mode ) . - pubmed
The globulin could be separated into two major subunits by sodiumdodecyl sulfate polyacrylamide gel electrophoresis . - pubmed
Retinol modulates site - specific mobility of apo - cellular retinol - binding protein to promote ligand binding . - pubmed
We have investigated the mechanism of retinol uptake by the cellular retinol - binding protein type I ( CRBP ) using line shape analysis of NMR signals . - pubmed
The highly similar structures of apo- and holo - CRBP exhibit closed conformations that seemingly offer no access to ligand , yet the protein binds retinol rapidly and with high affinity . - pubmed
An initial nonspecific encounter with the ligand induces the formation of long - lived conformers in the portal region of CRBP suggesting a mechanism how retinol accesses the cavity . - pubmed
The peroxisome proliferation - activated receptor gamma ( PPARgamma ) is mainly expressed in the adipose tissue and integrates the control of energy , lipid , and glucose homeostasis . - pubmed
The functionality of the receptor was evidenced by 15 - deoxy-12,14 - prostaglandin J ( 2 ) ( PGJ2 ) and rosiglitazone ( BRL ) PPARgamma - agonists that were tested on capacitation , acrosome reaction , and motility . - pubmed
Both treatments also increase AKT phosphorylations and influence glucose and lipid metabolism in sperm . - pubmed
Using transcription reporter assays , we show that single nucleotide polymorphisms in the PGIS promoter can affect transcriptional activity . - pubmed
These results show that an individual 's PGIS promoter haplotype can play an important role in the predisposition for lung cancer and CpG methylation provides an epigenetic mechanism for the down - regulated PGIS expression . - pubmed
RESULTS : Bone growth proceeded very rapidly ; at 1 day of age , the degree of mineralization ( phosphate / protein ratio ) , the density of mineralized bone ( TMD ) , and mineral crystallinity had reached 36 % , 51 % , and 87 % of the adult ( 450 days ) values , respectively . - pubmed
In contrast to the notable changes in mineralization , carbonate substitution into the mineral lattice ( carbonate / phosphate ratio ) and collagen cross - linking did not show any significant changes over this time period . - pubmed
Between samples of different time - points , significant positive correlations were observed between the elastic modulus and TMD ( r(2 ) = 0.84 ) , phosphate / protein ratio ( r(2 ) = 0.59 ) , and crystallinity ( r(2 ) = 0.23 ) , whereas collagen cross - linking showed a small but significant negative correlation ( r(2 ) = 0.15 ) . - pubmed
The ATP - binding cassette transporter subfamily A member 1 ( ABC - A1 ) and type 2 diabetes : an association beyond HDL cholesterol . - pubmed
The absence of ABC - A1 led to cholesterol accumulation within the beta cell plasma membrane , suggesting that cholesterol may play a role in the insulin secretory pathway . - pubmed
In conclusion , ABC - A1 may be more than a determinant of HDL - cholesterol . - pubmed
In addition , a profoundly decreased intracellular calcium flux in CD4(+)T cells after PHA stimulus was detected among 620Trp carriers . - pubmed
Improvement of an enzyme electrode by poly ( vinyl alcohol ) coating for amperometric measurement of phenol . - pubmed
A poly ( vinyl alcohol ) film cross - linked with glutaraldehyde ( PVA - GA ) was introduced to the surface of a tyrosinase - based carbon paste electrode . - pubmed
The coated PVA - GA film was beneficial in terms of increasing the stability and reproducibility of the enzyme electrode . - pubmed
The electrode showed a sensitive current response to the reduction of the o - quinone , which was the oxidation product of phenol , by the tyrosinase , in the presence of oxygen . - pubmed
The effects of the PVA and PVA - GA coating , the pH , and the GA : PVA ratio on the current response were investigated . - pubmed
The sensitivity of the PVA - GA - Tyr electrode was 130.56microA / mM ( 1.8microA / microM cm(2 ) ) and the linear range of phenol was 0.5 - 100microM. - pubmed
Using the PVA - GA - Tyr electrode , a two - electrode system was tested as a prototype sensor for portable applications . - pubmed
[ Study on the interaction of hesperidin or icariin with lysozyme by fluorescence spectroscopy ] . - pubmed
The interaction of hesperidin ( HES ) or icariin ( ICA ) and lysozyme ( LYS ) was studied by fluorescence spectroscopy in physiological buffer solution . - pubmed
It was observed that there was a strong fluorescence quenching effect of hesperidin or icariin on lysozyme . - pubmed
The quenching constants of the drugs with lysozyme were measured at different temperatures , and the quenching mechanism was suggested as dynamic quenching for HES - LYS system and both static and dynamic quenching for ICA - LYS system . - pubmed
The thermodynamic parameters of the interaction of hesperidin or icariin and lysozyme were measured according to the Van 's Hoff equation : the enthalpy change ( DeltaH ) and the entropy change ( DeltaS ) of HES - LYS system and ICA - LYS system were calculated to be 20.29 kJ x mol(-1 ) and 146.28 J x mol(-1 ) x K(-1 ) , and -3.47 kJ x mol(-1 ) and 81.16 J x mol(-1 ) x K(-1 ) , respectively , which indicated that the interaction of hesperidin and lysozyme was driven mainly by hydrophobic force , whereas the interaction of icariin and lysozyme was driven mainly by electrostatic force . - pubmed
The binding distances of hesperidin and icariin from the lysozyme tryptophan residue were calculated to be 1.34 nm and 1.24 nm , respectively , based on the Forster 's theory of non - radiation energy transfer . - pubmed
The results of synchronous fluorescence spectra showed that the binding of hesperidin or icariin to lysozyme induced conformational changes in lysozyme . - pubmed
Sizing up ethanol - induced plasticity : the role of small and large conductance calcium - activated potassium channels . - pubmed
Small ( SK ) and large conductance ( BK ) Ca ( 2 +)- activated K (+ ) channels contribute to action potential repolarization , shape dendritic Ca ( 2 +)spikes and postsynaptic responses , modulate the release of hormones and neurotransmitters , and contribute to hippocampal - dependent synaptic plasticity . - pubmed
Over the last decade , SK and BK channels have emerged as important targets for the development of acute ethanol tolerance and for altering neuronal excitability following chronic ethanol consumption . - pubmed
In this mini - review , we discuss new evidence implicating SK and BK channels in ethanol tolerance and ethanol - associated homeostatic plasticity . - pubmed
Findings from recent reports demonstrate that chronic ethanol produces a reduction in the function of SK channels in VTA dopaminergic and CA1 pyramidal neurons . - pubmed
There is also increasing evidence supporting the idea that ethanol sensitivity of native BK channel results from differences in BK subunit composition , the proteolipid microenvironment , and molecular determinants of the channel - forming subunit itself . - pubmed
Taken together , these studies suggest that SK and BK channels contribute to ethanol tolerance and adaptive plasticity . - pubmed
OBJECTIVES : This study sought to evaluate the frequency and efficacy of combination of bivalirudin and provisional glycoprotein ( GP ) IIb / IIIa blockade compared with bivalirudin monotherapy in current clinical practice of percutaneous coronary intervention ( PCI ) with drug - eluting stents ( DES ) . - pubmed
BACKGROUND : Previous randomized trials have demonstrated that a strategy of bivalirudin with provisional ( bailout ) GP IIb / IIIa inhibition was non - inferior to unfractionated heparin ( UFH ) plus planned GP IIb / IIIa blockade for the prevention of acute and long - term adverse clinical events . - pubmed
However , the frequency and efficacy of provisional GP IIb / IIIa inhibition in addition to the full - dose bivalirudin in current practice is not well established . - pubmed
The incidence of in - hospital mortality ( 0 % vs. 0.3 % p = 1.000 ) , MI ( 7.1 % vs. 6.6 % ; p = 0.864 ) , and the combined endpoint of death , stroke , emergent coronary artery bypass graft surgery ( CABG)/PCI , or MI ( 7.1 % vs. 6.9 % ; p = 0.868 ) were similar in the bivalirudin - plus - bailout GP IIb / IIIa inhibitor versus the bivalirudin - alone arm . - pubmed
There was a higher incidence of bleeding complications ( 16.0 % vs. 9.6 % ; p = 0.018 ) in the bivalirudin - plus - bailout GP IIb / IIIa versus the bivalirudin - alone group . - pubmed
At follow up , there were 4 ( 2.6 % ) deaths in the bivalirudin - plus - GP IIb / IIIa inhibitor group versus 83 ( 7.0 % ) deaths in the bivalirudin - alone arm ( HR 0.36 , 95 % confidence interval [ CI ] 0.13 - 0.98 ; p = 0.044 ) . - pubmed
After multivariate Cox regression analysis , bailout GP IIb / IIIa use in addition to bivalirudin was associated with similar long - term survival when compared to bivalirudin monotherapy ( HR 0.41 , 95 % CI 0.15 - 1.12 ; p = 0.081 ) . - pubmed
Efficacy of the treatment was assessed by measuring the histopathologic injury score ( HIS ) , and biochemically by determining tissue superoxide dismutase ( SOD ) , glutathione peroxidase ( GSH - Px ) , and malondialdehyde ( MDA ) in ileum , liver and kidney homogenates . - pubmed
Furthermore , we found that the C57BL/6J - like changes in anxiety - related behavior after restraint were absent in null mutants lacking the modulatory NMDA receptor subunit Grin2a , but not the AMPA receptor subunit Gria1 . - pubmed
The rate of hydrolysis of GXG with TXy and accellerase was increased by 15 - 20 % in the presence of chemical surfactants ( tween 80 and toluene ) or protein additive ( BSA ) . - pubmed
The fermentation of enzymatic hydrolysates of GXG by TXy and accellerase with free cells at 40 degrees C produced an ethanol yield of 0.39 and 0.41 g / g whereas with immobilized cells produced 0.45 and 0.43 g / g , respectively , with a theoretical conversion efficiencies of 78 - 88 % . - pubmed
RESULTS : Lower glucose infusion rate was significantly associated with lower levels of HDL cholesterol in youths with Type 1 diabetes and with higher levels of triglycerides and higher triglyceride / HDL ratio in both youths and adults . - pubmed
The phenolic compounds both in ethanol and water extracts of the plant were analysed using liquid chromatography - mass spectrometry ( LC - MS ) and capillary zone electrophoresis . - pubmed
The contents of the pharmaceutically important antidepressants hyperforin and hypericin were also estimated by LC - MS and compared with the data in the literature . - pubmed
After high - performance liquid chromatography analysis , we have 69.3 + -3.2 % of [ ( 18 ) F ] F (- ) incorporation ratio on the manual synthesis and applied these conditions to automatic preparation with GE TracerLab FX module . - pubmed
The pS422 antibody was used as an early tau pathology marker that labels tau phosphorylated at Ser422 ; the TauC3 antibody was used to detect later stage tau pathology . - pubmed
Methylation status of CpG sites in the MCP-1 promoter is correlated to serum MCP-1 in Type 2 diabetes . - pubmed
Bodymass index , blood pressure , blood lipid , blood glucose , glycosylated hemoglobin ( HbA1c ) , and serum MCP-1 were measured . - pubmed
Methylation status of CpG sites in theMCP-1 promoter was determined using methylation specific polymerase chain reaction . - pubmed
MCP-1 promoter methylation was significantly correlated to serum MCP-1 , HbA1c , fasting blood glucose , and triglyceride . - pubmed
CONCLUSION : These data suggest that hypomethylation of CpG sites in the MCP-1 promoter region may be affected by blood glucose and TG , which then increase the serum MCP-1 level and may play a role in the vascular complications of Type 2 diabetes . - pubmed
The primary end point was to assess differences in postoperative serum alanine , aspartate aminotransferase ( ALT and AST ) and bilirubin levels . - pubmed
The curves of serum AST , ALT and bilirubin levels in a span of time of five postoperative days were not significantly different between the three groups . - pubmed
The FBs were incubated with different concentrations of PDGF - AA and the changes in their proliferative activity were quantified with MTT assay . - pubmed
A novel series of pyrazolylpiperidine N - type calcium channel blockers . - pubmed
A novel series of pyrazole - based N - type calcium channel blockers was identified by structural modification of a high - throughput screening hit and further optimized to improve potency and metabolic stability . - pubmed
Die - off ratio correlates with increased TNF - alpha : IL-10 ratio and decreased IVF success rates correctable with humira . - pubmed
However , mutating putative GR binding sites did not substantially reduce induction of ras - dva promoter activity by corticosterone , suggesting additional DNA elements within the 5'-flanking region are responsible for glucocorticoid regulation . - pubmed
Decreased numbers of apoptotic type II pneumocytes and mesenchymal cells , and decreased contents of proapoptotic cleaved caspase-3 and - 7 and cytoplasmic cytochrome c , and an increase in antiapoptotic Bcl - xL were found in lungs treated with diphenyl phenyl diamine . - pubmed
The study was aimed to investigate the effect of steroid substitution on serum osteoprotegerin and receptor activator of nuclear factor kappa - beta ligand ( RANKL ) levels in relation to bone mineral density ( BMD ) in PAI . - pubmed
Serum osteoprotegerin increased with age ( p<0.001 ) , but showed no correlation with daily hydrocortisone dose . - pubmed
Osteoprotegerin was negatively correlated with serum dehydroepiandrosterone - sulfate ( p=0.008 ) and with BMD at the lumbar spine ( p<0.001 ) and femoral neck ( p=0.003 ) . - pubmed
RANKL correlated negatively with PAI duration ( p=0.029 ) and positively with daily hydrocortisone dose ( p=0.018 ) . - pubmed
In conclusion , increased osteoprotegerin in PAI might reflect a compensatory response to enhanced bone resorption due to exogenous steroid excess and/or result from a deficit in adrenal androgens . - pubmed
RANKL levels remain within normal range on standard steroid replacement . - pubmed
Perianth explants in a relatively early stage of development were competent for differentiation of tepals on Murashige and Skoog ( MS ) medium supplemented with 2 mg 1(-1 ) N ( 6 )- benzylaminopurine ( BAP ) or zeatin and 0.1 mg 1(-1 ) 2,4 - dichlorophenoxyacetic acid ( 2,4 - D ) . - pubmed
Alkaline phosphatase ( AP ) catalyses the detachment of phosphate residues from different substrates . - pubmed
The effect of different doses of AP ( 1.2 and 2.5 IU / mL ) on several sperm parameters after IVC ( viability , acrosome integrity with FITC - PSA , capacitation status with CTC staining , tyrosine phosphorylation ) and on fertilizing ability during IVF were also evaluated . - pubmed
Apoptosis was evaluated with annexin - V and propidium iodide staining and flow - cytometry . - pubmed
Ligand tuning of single - site manganese - based catalytic antioxidants with dual superoxide dismutase and catalase activity . - pubmed
A bio - inspired manganese ( ii ) complex with a linear pentadentate ligand framework containing soft sulfur donors and an alternating NSNSN binding motif displays excellent dual CAT / SOD - like antioxidant activity with high turnover efficiency and good operation stability in an aqueous environment . - pubmed
A nanowire - directed templating synthesis of metal - organic framework ( MOF ) nanofibers has been demonstrated , where ultrathin tellurium nanowires ( TeNWs ) with excellent dispersivity can act as templates for directed growth and assembly of ZIF-8 nanocrystals ( one typical MOF ) , resulting in the formation of uniform ZIF-8 nanofibers . - pubmed
These terminal oxidases have varying properties such as their affinity for oxygen , transcriptional regulation and proton pumping ability . - pubmed
In comparison with other oxidase mutants , the cyo mutant had a significantly longer lag phase under low - oxygen conditions . - pubmed
Using a cyo : : lacZ transcriptional fusion , it was shown that cyo expression in the wild type peaks between 1 and 2.5 % O2 . - pubmed
In addition , it was shown with quantitative reverse transcriptase PCR that cyoB is upregulated approximately fivefold in 1 % O2 compared with fully aerobic ( 21 % O2 ) conditions . - pubmed
Although it is commonly thought that Cyo is utilized only at higher oxygen concentrations , the results from this study indicate that Cyo is important for adaptation to and sustained growth under low oxygen . - pubmed
Their stimulation by specific testosterone albumin conjugates ( TAC ) activate rapidly non - genomic FAK / PI3K / Rac1 / Cdc42 signaling , trigger actin reorganization and inhibit cell motility . - pubmed
In line with this , TAC and EMD638683 activated caspase-3 . - pubmed
These effects were insensitive to the classical androgen receptor ( iAR ) antagonist flutamide , pointing to iAR - independent , mAR - induced responses . - pubmed
Blocking actin restructuring with cytochalasin B abrogated the TAC- and EMD638683 - induced pro - apoptotic responses . - pubmed
Our aims were , to clarify the blood lipid differences [ Total serum cholesterol ( TC ) , High - density lipoprotein ( HDL ) , Low density lipoprotein ( LDL ) , Triglyceride ( TG ) ] between female patients who had attempted suicide and controls and to determine whether we could use the patients initial lipid profiles to predict suicide re - attempt within the subsequent year . - pubmed
Blood lactate rose from DIA1 to DP to DIA2 . - pubmed
O2 extraction was attenuated during DP ( P & lt ; 0.05 ) , but was the same during DIA1 and DIA2 . - pubmed
The ratio of upper-/whole - body EMGRMS correlated negatively with O2 extraction in the arms during both sessions of DIA ( P & lt ; 0.05 ) . - pubmed
These three new criteria are : greater than 60 % clonal plasma cells on bone marrow biopsy , a serum free light chain ( sFLC ) ratio of & gt;100 ( the involved sFLC must be & gt;100 mg / l ) and greater than one unequivocal focal lesion on advanced imaging ( low dose whole body computerized tomography , magnetic resonance imaging , ( 18 ) F fluorodeoxyglucose positron emission tomography ) . - pubmed
Before and after treatment , serum estradiol ( E2 ) , testosterone ( T ) , luteinizing hormone ( LH ) , prolactin ( PRL ) by radioimmunoassay and immunoglobulin ( IgG ) by immunodiffusion , erythrocyte sedimentation rate ( ESR ) by Westergren , interferon - gamma ( IFN - gamma ) and interleukin-4 ( IL-4 ) by enzyme linked immunosorbent assay were determined . - pubmed
GalN caused more severe injury to steatotic hepatocytes than to lean cells as documented by lactate dehydrogenase leakage . - pubmed
Necrotic mode of cell death strongly prevails over apoptosis since we did not observe any significant increase in activities of caspase 3 , 8 and 9 in any group of hepatocytes treated with GalN. - pubmed
Impact Study : MK-0646 ( Dalotuzumab ) , Insulin Growth Factor 1 Receptor Antibody Combined with Pemetrexed and Cisplatin in Stage IV Metastatic Non - squamous Lung Cancer . - pubmed
Dalotuzumab - MK0646 ( D ) is a humanized monoclonal antibody that targets IGF-1R. - pubmed
Serum glucose , insulin and amyloid A were measured 24 h before operation and at endpoints . - pubmed
Bodyweight was increased by HFD , whereas serum glucose , amyloid A and insulin were not influenced . - pubmed
TAT Peptide - Conjugated Magnetic PLA - PEG Nanocapsules for the Targeted Delivery of Paclitaxel : In Vitro and Cell Studies . - pubmed
The conjugation of a cysteine - modified TAT peptide ( HCys - Tyr - Gly - Arg - Lys - Lys - Arg - Arg - Gln - Arg - Arg - Arg - NH2 ) on the surface of the nanocapsules resulted to highly increased uptake of nanocapsules by cancer cells , as well as to profound improvement of their cytotoxicity against the cancer cells . - pubmed
In this study we assessed the pain score - VAS at rest at 2 , 4 , 6 , 12 and 24 hours after the operation and the total analgesic consumption of morphine over 24 hours . - pubmed
The cumulative 24 hours morphine consumption after the operation was significantly lower in group III ( 5.53 1.21 mg ) than in group II ( 6.16 2.41 mg ) and group I ( 9.26 2.41 mg ) . - pubmed
CONCLUSION : Concerning the inguinal hernia repair we found better postoperative pain scores and 24 hours reduction of the morphine consumption in group III ( GA and TAP - D block ) compared with group I ( GA ) and group II ( GA + TAP block ) . - pubmed
The claudin-4 mediated chloride permeability can be regulated by a protease endogenously expressed by the collecting duct cell - channel - activating protease 1 . - pubmed
Deletion of either claudin protein from the collecting duct causes renal chloride reabsorption defects and low blood pressure . - pubmed
Corneal edema was significantly less and the increases in anti - oxidative HO-1 , CAT and SOD2 mRNA expressions were significantly suppressed in the H2 group . - pubmed
In addition , corneal endothelial cell expressions of two oxidative stress markers , 4 - HNE and 8 - OHdG , were significantly lower in the H2 group . - pubmed
We found that macrophage colony - stimulating factor - dependent macrophage differentiation and subsequent receptor activator of NF - kappaB ligand - induced osteoclast formation were largely suppressed by adding TAS-115 . - pubmed
The phosphorylation of the macrophage colony - stimulating factor receptor FMS and osteoclast related kinases such as ERK and Akt were also suppressed by the presence of TAS-115 . - pubmed
RESULTS : Naftopidil treatment reduced the upregulation of mRNA levels of ischemia and fibrosis markers at the early phase of SCI , and ameliorated the decrease of bladder compliance and voiding efficiency , and the increase of urethral pressure and collagen concentration in the bladder wall at the late phase of SCI . - pubmed
Tadalafil treatment reduced the upregulation of mRNA levels of fibrosis markers , the decrease of bladder compliance and the increase of collagen concentration at the late phase of SCI . - pubmed
CONCLUSIONS : These results suggest that naftopidil and tadalafil treatments improved the bladder remodeling shown by increased bladder collagen contents after SCI in a different time course . - pubmed
BACKGROUND : Celecoxib is a selective cyclo - oxygenase-2 inhibitor recommended orally to treat arthritis and osteoarthritis . - pubmed
OBJECTIVES : We tested the hypothesis that nicotine acting at nicotinic acetylcholine receptors ( nAChRs ) would augment Pavlovian alcohol - seeking . - pubmed
RESULTS : All paired groups acquired conditioned port - entry responding to the CS , indicative of Pavlovian alcohol - seeking , whereas unpaired control group did not . - pubmed
Pre - session nicotine injections increased CS port - entries relative to saline , only in the paired group . - pubmed
This nicotine - induced enhancement of Pavlovian alcohol - seeking was blocked by pre - treatment with the nAChR antagonist mecamylamine . - pubmed
Photobiosynthesis of stable and functional silver / silver chloride nanoparticles with hydrolytic activity using hyperthermophilic beta - glucosidases with industrial potential . - pubmed
The syntheses were straightforward and rapid processes carried out by mixing beta - glucosidase and silver nitrate ( in buffer 10mM Tris - HCl , pH 8) under irradiation with light ( over a wavelength range of 450 - 600 nm ) , therefore , compatible with the green chemistry procedure . - pubmed
Finally , the results showed that the enzymatic activities were maintained in the beta - glucosidases assisted Ag / AgCl - NPs . - pubmed
Although the pilocarpine - induced SE itself is not significantly reduced , the SE - induced neuronal cell death is significantly reduced in the CA1 and the lateral septum , indicating that TRPC1 / 4 channels directly contribute to SE - induced neuronal cell death . - pubmed
The use of LABA+LAMA association without ICS increased from 0.9 to 7 % , but remained lower than the fixed LABA+ICS association ( 26 % ) , less often prescribed than the triple association LABA+ICS+LAMA ( 32.5 % in period 2 ) . - pubmed
AIM : The aim of this study is to identify the potential role of high protein - low carbohydrate ketogenic diet ( KD ) in managing weight loss in patients who underwent gastric banding and did n't lose weight anymore . - pubmed
RESULTS : An in vitro assay for the glycation of bovine serum albumin by methylglyoxal showed that GS - E3D inhibited AGE formation at an IC50 value of 19.65 + - 4.35 mug / mL. - pubmed
A novel ferrocene - based substrate for the ratiometric electrochemical detection of beta - galactosidase was designed and synthesised . - pubmed
Herein , well - defined hollow dendritic AuPtAg alloyed nanocrystals ( ANCs ) were synthesized by a simple L - proline - mediated one - pot aqueous method . - pubmed
The resultant label - free immunosensor exhibited the improved performance for highly sensitive detection of PSA based on the enhanced catalytic currents of K3 [ Fe ( CN ) 6 ] as a signal probe . - pubmed
An increase in auxin level in the root tips was associated with increased expression of auxin biosynthesis genes and auxin polar transporter AUX1 and PIN2 under BA stress . - pubmed
Furthermore , BA stimulated ethylene evolution , whereas blocking BA - induced ethylene signaling with ethylene biosynthesis inhibitor ( Co2 + ) , ethylene perception antagonist ( 1 - MCP ) or ethylene signaling mutant lines etr1 - 3 and ein3eil1 compromised BA - mediated inhibition of root elongation , up - regulation of auxin biosynthesis - related genes and AUX1 and PIN2 , indicating that ethylene signal was involved in auxin - mediated inhibition of primary root elongation during BA stress . - pubmed
Taken together , our results reveal that allelochemical BA inhibits root elongation by increasing auxin accumulation by stimulating auxin biosynthesis and AUX1 / PIN2 - mediated auxin transport via stimulating ethylene production and an auxin / ethylene independent ROS burst . - pubmed
The EOs was also found to be effective in reducing virulence factors like staphyloxanthin and hemolysin . - pubmed
The results suggest that ( E )- nerolidol showed better binding affinity towards the enzyme . - pubmed
Correction : Synergistic Interaction between the HDAC Inhibitor , MPT0E028 , and Sorafenib in Liver Cancer Cells In Vitro and In Vivo . - pubmed
RATIONALE : High - precision determination of magnesium ( Mg ) isotopes can now be routinely achieved by multi - collector inductively coupled plasma mass spectrometry ( MC - ICP - MS ) . - pubmed
Determination of elevated levels of nitrate in vegetable powders by high - precision isotope dilution GC - MS . - pubmed
A high - precision isotope dilution GC - MS method was employed for the determination of nitrate in processed vegetables . - pubmed
The samples were extracted in water , derivatized with triethyloxonium tetrafluoroborate and analyzed by headspace GC - MS ( 15 samples / h ) . - pubmed
We then describe our efforts to apply site - specific 1D and 2D IR spectroscopy toward elucidation of protein conformations and dynamics to investigate their involvement in protein molecular recognition , in particular mediated by dynamic complexes : plastocyanin and its binding partner cytochrome f , cytochrome P450s and substrates or redox partners , and Src homology 3 domains and proline - rich peptide motifs . - pubmed
However , the few prior studies exclusively relied on spot measurements of phasic HPA - axis activity , which may not adequately capture cortisol output over prolonged periods of time . - pubmed
CONCLUSION : In conjunction with data from previous studies , it is thus tempting to speculate that sGC may rather affect the capacity of dynamic changes and flexible adaption of an individual 's HPA - axis than changes in tonic steroid output . - pubmed
The TS stabilization of up to ~10 kcal / mol was consistent with the formation of a strong n pi * stabilization between the imide carbonyl oxygens and the ortho R group in the planar TS . - pubmed
beta - Etherases are enzymes with a promising potential for application in lignin depolymerization due to their selectivity in the cleavage of beta - O-4 aryl ether bonds . - pubmed
TMAO levels after 6 months of l - carnitine supplementation were associated with higher low - density lipoprotein - cholesterol ( LDL - c ) ( Spearman Rho = 0.518 , p = 0.003 ) and total cholesterol ( TC ) ( Spearman Rho = 0.407 , p = 0.026 ) levels . - pubmed
D - loop methylation was not directly correlated to the TMAO augmentation observed in the supplemented group , but its increase inversely correlated with TC ( Pearson coefficient = -0.529 , p = 0.029 ) and LDL - c ( Pearson coefficient = -0.439 , p = 0.048 ) . - pubmed
Overall , no significant difference in mortality was observed between the TP and catecholamine groups ( risk ratio(RR ) , 0.85 ( 0.70 to 1.03 ) ; P = 0.09 ) . - pubmed
DEGs were mainly enrich in ' sterol biosynthetic and metabolic process ' , ' cholesterol metabolic and biosynthetic process ' by GO enrichment analysis . - pubmed
The effects of high - intensity ultrasound ( HIU ) treatment ( 20 kHz at 400 W , 0 , 5 , 20 , and 40 min ) on the physical , structural , and aggregation properties of DGH were investigated with the aim to reveal the influence of the selectively hydrolyzing glycinin component on the HIU treatment of soy protein . - pubmed
Our objective was to assess the effect of nitric oxide added to the sweep gas of the oxygenator during cardiopulmonary bypass ( CPB ) in infants on platelet count , platelet function , clinical outcomes , and safety . - pubmed
Nitric oxide added to the sweep gas of the oxygenator during CPB in infants did not have an appreciable effect on preservation of platelet count , platelet responsiveness to agonist , or clinical outcomes . - pubmed
In contrary , caveolin-1 depletion shows no effect on the expression , phosphorylation ( on sites Ser39 , Ser56 , and Ser83 ) , distribution , solubility , and cellular dynamics of vimentin filaments . - pubmed
Uterine PGR responds to the heightened levels of ovarian progesterone ( P4 ) after ovulation and regulates uterine gene transcription for successful embryo implantation . - pubmed
This study aimed to synthesize and characterize L - epigallocatechin gallate ( EGCG ) complexed Mn2 + nanoparticle ( L - EGCG - Mn ) , a proof - of - concept pH - sensitive manganese core nanoparticle ( NP ) , and compare its magnetic resonance ( MR ) properties with those of Gd - DTPA , both in vitro and in vivo . - pubmed
It was suggested that the inhibition of prostaglandin synthesis by flurbiprofen might be due to the inhibition of the endoperoxygenase which catalyzed conversion of arachidonic acid to cyclic endoperoxide . - pubmed
In contrast , phenylbutazone which was a pyrozolone derivative inhibited the formation of prostaglandin E2 , but not affected the endoperoxygenase reaction . - pubmed
The kinetic studies for inhibition of prostaglandin E2 synthetase indicated that flurbiprofen competitively inhibited prostaglandin E2 synthesis , just like indomethacin . - pubmed
Cytochalasin B , which inhibits phagocytosis , acts by interfering with the interaction between actin - binding protein and actin . - pubmed
Conversely both hydrazono compounds decreased the monoamine oxidase activity much less , than phenelzine . - pubmed
CHEHP ( 145 mumol / kg ) inhibited this enzyme by less than 14 % . - pubmed
Plasma renin activity ( PRA ) and PRA responsiveness to sodium depletion were normal , suggesting that extracellular fluid volume was not increased . - pubmed
Relationships between plasma renin activity and urinary and plasma catecholamines . - pubmed
Effect of ergot drugs on central 5 - hydroxytryptamine neurons : evidence for 5 - hydroxytryptamine release or 5 - hydroxytryptamine receptor stimulation . - pubmed
It was found that ergocornine increased the 5 - HT receptor activity independent of presynaptic 5 - HT stores and that it didnot have any effects on uptake , retention and spontaneous overflow of 3 - H-5 - HT in vitro but reduced the fiedl stimulation - induced release of 3 - H-5 - HT in vitro . - pubmed
Therefore , it is suggested that ergocornine is a 5 - HT recpetor - stimulating agent , an effect which may lead to reduction of nervous impulse flow in the 5 - HT neurons and subsequently of 5 - HT release and turnover . - pubmed
MPME , on the other hand , seems to increase 5 - HT receptor release of 5 - HT stores , mainly from extragranular sites . - pubmed
Inhibition of the 5 - HT membrane pump does not seem to be of any major importance , since chlorimipramine was only weakly active on the extensor reflex in the pharmacological models used and since MPME did not block but rather enhanced the 5 - HT depletion caused by 4 - methyl - alpha - ethyl - m - tyramine . - pubmed
It is suggested that MPME is a releaser of extragranular 5 - HT stores leading to increased 5 - HT receptor activity and reduction of 5 - HT turnover in the same way as indicated for ergocornine . - pubmed
In spite of the fact that in carbon - limited chemostat cultures under induced conditions the enzymes involved in methanol metabolism were present at wild - type ( WT ) levels , this mutant is unable to grow on methanol as a sole carbon and energy source . - pubmed
Addition of methanol to glucose - limited ( SR = 12.5 mM ) chemostat cultures of the PER mutant only resulted in an increase in yield when small amounts were used ( up to 22.5 mM ) . - pubmed
The enzyme patterns obtained suggested that the ultimate decrease in yield observed at enhanced methanol concentrations was due to an inefficient methanol - metabolism as a consequence of the absence of peroxisomes . - pubmed
The absence of intact peroxisomes results in two major problems namely i ) in H2O2 - metabolism , which most probably is no longer mediated by catalase and ii ) the inability of the cell to control the fluxes of formaldehyde , generated from methanol . - pubmed
Nicotinic acid and its derivatives block adipose tissue lipolysis thus reducing the FFA flux of free fatty acids to the liver and the synthesis of very - low - density lipoproteins ( VLDL ) . - pubmed
Clofibrate and its derivatives decrease the synthesis of VLDL and increase their catabolism . - pubmed
Lipoprotein lipase activity as well as VLDL fractional catabolic rate are increased by fibrates . - pubmed
On the mechanism of rotenone - insensitive reduction of quinones by mitochondrial NADH : ubiquinone reductase . - pubmed
NADH acts as an incomplete competitive inhibitor for 5,8 - dioxy-1,4 - naphtoquinone during its rotenone - insensitive reduction by mitochondrial NADH : ubiquinone reductase . - pubmed
The bimolecular rate constant of the reduction of the enzyme by NADH in the quinone reductase reaction is about 2 times less than that of ferricyanide reductase reaction . - pubmed
It seems that during the steady - state reduction of ferricyanide and 5,8 - dioxy-1,4 - naphtoquinone these oxidizers react with NADH : ubiquinone reductase reduced to different extents . - pubmed
Interaction of 1,25 - dihydroxyvitamin D and plasma renin activity in high renin essential hypertension . - pubmed
Renin secretion by the kidney is inhibited by an increase in free intracellular calcium concentration . - pubmed
An inverse correlation existed between the change in vitamin D and the change in plasma renin activity ( r = -0.765 , P = .01 ) . - pubmed
It is postulated that the increase in dietary sodium led to an increase in serum 1,25 - dihydroxyvitamin D concentration , which may have contributed to an increase in intracellular calcium concentration , a decrease in renal secretion of renin , and a fall in plasma renin activity . - pubmed
Therefore , 1,25 - dihydroxyvitamin D may be a mediator in the response of high renin hypertension to increased sodium intake . - pubmed
One of the genes , apparently by means of alternate splicing , gives rise to two prothymosin alpha mRNAs , one of which has an additional internal glutamic acid codon with respect to the other . - pubmed
It was demonstrated on short columns packed with these anion exchangers that ( i ) a substantially higher resolution of proteins and nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers ; ( ii ) the low external surface area of the non - porous anion exchanger is not a critical parameter in analytical separations and ( iii ) microgram - amounts of enzymes of high purity and full biological activity were isolated . - pubmed
Preparations of monoclonal antibodies directed against some blood group antigens and anti - A1 lectin reagent were devoid of spectrin autoantibody as expected . - pubmed
Care must be exercised in studies designed to determine the association of some blood group antigens with the red cell membrane skeleton , when using polyclonal sera which contain spectrin autoantibodies in addition to the antibodies against the blood group antigen in question . - pubmed
Recently Cyclosporin A has been shown to cause renal damage , which correlates with increased glomerular neutrophil migration . - pubmed
Lower peptides and amino acids in hydrolyzates of casein obtained with protosubtilin were determined using high pressure liquid chromatography ( HPLC ) . - pubmed
Threshold of nickel sensitivity and immunoglobulin E determinations . - pubmed
hCRF caused brief pulse - like elevations of plasma IR - ACTH and cortisol without any effect on the plasma concentrations of GH , PRL , and LH . - pubmed
This is in contrast to oCRF , which causes prolonged ACTH and cortisol secretion . - pubmed
The oxidation of ferrocytochrome c mediated by cytochrome c oxidase was investigated in the presence of ferricytochrome c , trifluoroacetyl - cytochrome c , the heme fragments Hse65 -[ 1 - 65 ] and Hse80-[1 - 80 ] and their respective porphyrin derivatives , as well as carboxymethylated apoprotein and related fragments , polycations , salts and neutral additives . - pubmed
The protein content of insect venoms was determined using a Coomassie blue dye binding assay as well as the Lowry phenol assay . - pubmed
Basal plasma insulin before pregnancy was elevated and there was no rise from glucose ; after parturition the basal and pattern of response was normal . - pubmed
Correlation between effects of 24 different cytochalasins on cellular structures and cellular events and those on actin in vitro . - pubmed
The following effects were tested for each of 24 cytochalasins ; ( a ) four high dose ( 2 - 20 muM ) effects on the cellular level : rounding up of fibroblastic cells , contraction of actin cables , formation of hairy filaments containing actin , and inhibition of lymphocyte capping ; ( b ) a low dose ( 0.2 - 2 muM ) effect : inhibition of membrane ruffling ; and ( c ) two in vitro effects : an inhibition of actin filament elongation ( the high affinity effect [ low dose effect ] in vitro ) and an effect on viscosity of actin filaments(the low affinity effect [ high dose effect ] in vitro ) . - pubmed
From the data obtained with the 24 cytochalasins , we have calculated correlation coefficients of 0.87 and 0.79 between an effect in vivo , inhibition of capping , and an effect in vitro , inhibition of actin filament elongation , as well as between inhibition of capping and another effect in vitro , effect on viscosity of actin filaments , respectively . - pubmed
The strong positive correlation among low and high dose effects in vivo and those in vitro suggests that most of the effects caused by a cytochalasin , irrespective of doses or affected phenomena , might be attributed to the interaction between the drug and the common target protein , actin . - pubmed
In control cells with functional classic cadherins , we observed redistribution of desmoplakin to cell - cell borders with insertions of keratin filaments at the contact sites as soon as 2 h after calcium elevation , after an earlier event of E - cadherin translocation to the cell - cell contact sites . - pubmed
These findings indicate that the disruption of classic cadherin affected the organization of desmosomes upon calcium elevation and suggest that the proper function of classic cadherins is a prerequisite for desmosome assembly in keratinocytes . - pubmed
The effect of ethyl-2 -[ 6 -( 4 - chlorophenoxy ) hexyl ) oxirane-2 - carboxylate ( etomoxir ) and its oxirane analogues on the expression of several genes from liver and testis as well as the beneficial effect of etomoxir on heart performance and myosin isozyme expression is reviewed . - pubmed
The simultaneous addition of etomoxir and a fat diet produces an increase in the expression of carnitine palmitoyl transferase ( CPT ) I , cytochrome P-450 4A1 omega - hydroxylase and fatty acid binding protein ( L - FABP ) . - pubmed
The mRNA levels of other genes such as CPT II , 3 - hydroxy-3 - methylglutaryl - CoA ( HMG - CoA ) reductase , and fatty acid synthase ( FAS ) are increased by etomoxir alone . - pubmed
Neither cytosolic nor mitochondrial HMG - CoA synthase have any significant effect on the mRNA levels induced by etomoxir . - pubmed
A probably frequent mechanism for the action of etomoxir may involve the overload of non - metabolized fatty acids produced after the inhibition of CPT I by the oxirane compounds . - pubmed
There is some speculation as to whether the peroxisome proliferator activated receptor ( PPAR ) increases its participation in the expression , under the action of etomoxir . - pubmed
The presence of numerous nitric oxide synthase - positive neurons , all along the colon and at both plexuses , supports the hypothesis that an excessive production of nitric oxide may cause the persistent inhibition of contractions . - pubmed
The N - terminally truncated peptide ( 95 - 107 ) was also cleaved at that bond by both proteinases , but far less rapidly than peptide 91 - 107 , and additional cleavages appeared at secondary sites i.e those generating atriopeptin III ( R101 ) and auriculin ( R102 ) in rat pro - ANP . - pubmed
Particular ability of liver P450s3A to catalyze the oxidation of N omega - hydroxyarginine to citrulline and nitrogen oxides and occurrence in no synthases of a sequence very similar to the heme - binding sequence in P450s . - pubmed
Specific inhibitors of P450s3A as troleandomycin and dihydroergotamine strongly inhibit NOHA oxidation whereas metyrapone , an inhibitor of other P450 subfamilies , was without effect . - pubmed
These data show the particular ability of P450s of the 3A subfamily to catalyze the second step of the oxidation of L - arginine by NO synthases ( NOS ) . - pubmed
This analogy between NOSs and P450s3A is further substantiated by a protein sequence comparison which shows that a 9 - amino acid segment present in all NOSs exhibits a strong similarity with the sequence mainly responsible for heme binding in P450s3A which is well conserved in all P450s . - pubmed
This segment contains all the structural factors which are thought to be crucial for heme binding in P450s . - pubmed
[ 2 - 3H ] Glucosamine was prepared by acid hydrolysis of the labelled N - acetylglucosamine and was converted to [ 2 - 3H ] glucosamine 6 - phosphate by incubation with hexokinase and ATP . - pubmed
The sugar phosphate was used as a substrate for glucosamine 6 - phosphate deaminase ( isomerase , EC 5.3.1.10 ) in a simple 3H2O release assay . - pubmed
Endothelin ( ET ) , atrial natriuretic peptide ( ANP ) , catecholamine ( epinephrine , norepinephrine ) plasma levels , and ACE activity were measured from arterial blood sampled before injection of enalaprilat or NaCl solution ( baseline values ) , 10 min and 30 min thereafter , immediately before the start of cardiopulmonary bypass ( CPB ) , immediately after CPB , and at the end of surgery . - pubmed
Binding was also reduced by pretreatment with trypsin and papain , and by 8 - anilino-1 - naphthalenesulphonic acid , which binds to hydrophobic amino acids . - pubmed
Palladium-103 : a new radioactive source in the treatment of unresectable carcinoma of the pancreas : a phase I - II study . - pubmed
After considering some of the advantages of Pd-103 over I-125 , we designed a phase I - II clinical trial to assess the feasibility of intraoperative Pd-103 in unresectable carcinoma of the pancreas to study the related morbidity when combined with chemotherapy and external beam radiation , and to evaluate the impact on palliation and local control rates . - pubmed
The mean number of Pd-103 pellets was 45 ; the mean total activity to obtain a matched peripheral dose ( MPD ) of 11,000 cGy was 68.9 mCi . - pubmed
Activity of Parthenolide at 5HT2A receptors . - pubmed
These results suggest that parthenolide may be a low - affinity antagonist at 5HT receptors ; it is unlikely that the entire mechanism of action can be explained by its modest 5HT2A receptor affinity . - pubmed
Our data suggest a model whereby local production of phosphatidylinositol phosphates may trigger the binding of vesicles to the active zone via the Mint . - pubmed
From its periplasmic location , Cu , Zn SOD was inferred to acquire its substrate from outside the bacterial cell and was speculated to play a role in preserving meningococci from the action of microbicidal oxygen free radicals produced in the context of host defense . - pubmed
A sodC mutant was constructed by allelic exchange and was used to investigate the role of Cu , Zn SOD in pathogenicity . - pubmed
The mutant showed no increased sensitivity to paraquat , which generates superoxide within the cytosol , but was approximately 1,000 - fold more sensitive to the toxicity of superoxide generated in solution by the xanthine / xanthine oxidase system . - pubmed
We conclude that periplasmic Cu , Zn SOD contributes to the virulence of Neisseria meningitidis , most likely by reducing the effectiveness of toxic oxygen host defenses . - pubmed
METHODS : Qualitative morphological study on Rb was performed by means of light microscope , electron microscope and TdT mediated biotin - dUTP nick - end labeling ( TUNEL ) . - pubmed
While Panpal did not significantly influence the acetylcholinesterase activity in brain following non - treated as well as treated soman poisoning . - pubmed
Panpal increased so many - induced acetylcholinesterase inhibition following non - treated poisoning and decreased the reactivating effect of the oxime HI-6 following treated soman poisoning in diaphragm . - pubmed
CONCLUSIONS : Our data confirm the importance of the combination of reversible acetylcholinesterase inhibitor pyridostigmine with anticholinergic drugs in the pharmacological prophylaxis of soman poisoning because of the elimination of consequences of pyridostigmine - induced increasing in acetylcholinesterase inhibition in the peripheral compartment . - pubmed
The potency of the responses to other nucleotides : UTP & gt;= ATP & gt ; ADP & gt;> ; AMP = adenosine indicates the involvement of a mixture of P2Y receptors . - pubmed
Red blood cells of weak D phenotype have a much reduced number of presumably complete D antigens that were repeatedly reported to carry the amino acid sequence of the regular RhD protein . - pubmed
Likewise , in solid systems obtained by the solvent and melting solvent methods the drug solidifies in form III in ethanol / water and methanol while polymorph IV crystallizes in chloroform . - pubmed
Vasoactive intestinal polypeptide ( VIP ) and nitric oxide ( NO . ) - pubmed
N ( omega )- nitro - L - arginine and hemoglobin inhibited the rapid component but affected the sustained component less . - pubmed
The calcitonin gene - related peptide ( CGRP ) antagonist CGRP -( 8 - 37 ) did not alter EFS - induced LES relaxation . - pubmed
A high positive charge is the common characteristic shared by the beta - sheet region of stromal cell - derived factor-1 ( SDF-1 ) and CXCR4 antagonists such as ALX40 - 4C consisting of nine D - arginines . - pubmed
In the first study , peptide analogs possessing amino acid sequences from both the N - terminus and the beta - sheet region of SDF-1 were used as models to study the functional role of the beta - sheet region of SDF-1 . - pubmed
In the second study , two peptides containing nine arginines and the N - terminal signal sequence of SDF-1 were used as models to study the receptor binding mechanism of CXCR4 antagonists of high positive charges such as ALX40 - 4C. - pubmed
One peptide did not show signaling activity as indicated by the lack of calcium influx while another peptide induced unusual calcium influx distinct from that induced by the SDF-1 N - terminal peptide . - pubmed
Sp1 and NF - Y synergistically mediate the effect of vitamin D ( 3 ) in the p27 ( Kip1 ) gene promoter that lacks vitamin D response elements . - pubmed
Consistent with the roles of these transcription factors , treatment with vitamin D ( 3 ) stimulated the DNA binding activities of these factors to each element and induced the change of one NF - Y subunit . - pubmed
Importantly , the sMR has maintained its mannose - binding properties since it was capable of binding a mannosylated ligand . - pubmed
The high amount of sMR released by DC and its ability to bind mannosylated ligand might indicate that this molecule plays a role in the transport of mannosylated proteins from the site of inflammation to other parts of the body . - pubmed
Moreover , nicotinic survival support was not further enhanced by fibroblast growth factor , as seen for KCl , but increased to 100 % by adding PACAP , a trophic neuropeptide present in the ganglion . - pubmed
Total magnesium , ionized magnesium , albumin , ionized calcium , potassium , and pH did not change significantly by the administration of 1 g of magnesium sulfate . - pubmed
The cerebral blood flow was analyzed using 99mTc - ECD - SPECT . - pubmed
Applications of nylon membrane arrays to gene expression analysis . - pubmed
Kinesin is a molecular motor that interacts with microtubules and uses the energy of ATP hydrolysis to produce force and movement in cells . - pubmed
From the polarization experiments with the bifunctional probe , we determined the orientation of kinesin bound to microtubules in the presence of AMP - PNP and found close agreement with previous models derived from cryo - electron microscopy . - pubmed
We also compared the polarization anisotropy of monomeric and dimeric kinesin constructs bound to microtubules in the presence of AMP - PNP . - pubmed
rHuIL-12 was generally well tolerated , but was associated with temporary decreases in neutrophils and lymphocyte counts , and with elevations in serum transaminases and bilirubin . - pubmed
ERCC1 and thymidylate synthase mRNA levels predict survival for colorectal cancer patients receiving combination oxaliplatin and fluorouracil chemotherapy . - pubmed
PURPOSE : To test the hypotheses of whether the relative mRNA expression of the thymidylate synthase ( TS ) gene and the excision cross - complementing ( ERCC1 ) gene are associated with response to and survival of fluorouracil ( 5 - FU )/ oxaliplatin chemotherapy in metastatic colorectal cancer . - pubmed
CONCLUSION : These data suggest that intratumoral ERCC1 mRNA and TS mRNA expression levels are independent predictive markers of survival for 5 - FU and oxaliplatin combination chemotherapy in 5 - FU - resistant metastatic colorectal cancer . - pubmed
Estrogen action via the G protein - coupled receptor , GPR30 : stimulation of adenylyl cyclase and cAMP - mediated attenuation of the epidermal growth factor receptor - to - MAPK signaling axis . - pubmed
Estrogen triggers rapid yet transient activation of the MAPKs , extracellular signal - regulated kinase ( Erk )- 1 and Erk-2 . - pubmed
We have reported that this estrogen action requires the G protein - coupled receptor , GPR30 , and occurs via Gbetagamma - subunit protein - dependent transactivation of the epidermal growth factor ( EGF ) receptor through the release of pro - heparan - bound EGF from the cell surface . - pubmed
Here we investigate the mechanism by which Erk-1 /- 2 activity is rapidly restored to basal levels after estrogen stimulation . - pubmed
Evidence is provided that attenuation of Erk-1 /- 2 activity by estrogen occurs via GPR30 - dependent stimulation of adenylyl cyclase and cAMP - dependent signaling that results in Raf-1 inactivation . - pubmed
Moreover , GPR30 - dependent , cAMP - mediated attenuation of EGF - induced Erk-1 /- 2 activity was achieved by ER antagonists such as tamoxifen or ICI 182 , 780 ; yet not by 17alpha - E2 or progesterone . - pubmed
Thus , our data delineate a novel mechanism , requiring GPR30 and estrogen , that acts to regulate Erk-1 /- 2 activity via an inhibitory signal mediated by cAMP . - pubmed
Coupled with our prior findings , these current data imply that estrogen balances Erk-1 /- 2 activity through a single GPCR via two distinct G protein - dependent signaling pathways that have opposing effects on the EGF receptor - to - MAPK pathway . - pubmed
Conversely , the calcium - regulating receptors P2X ( 1 - 3 ) and P2Y ( 2 ) ( found in normal but not neoplastic skin ) were fully de - expressed within 2 microm of the melanoma but fully expressed beyond that distance . - pubmed
The effect of substituted 1,4 - anthraquinones on the photochemical activity and chlorophyll fluorescence of thylakoid membranes was examined . - pubmed
Stronger quinone - induced quenching of the chlorophyll fluorescence than quinone - induced changes in the activity of photosystem II is observed . - pubmed
The type ( Cl or Br ) and the position ( Cl ) of the chalogen atom strongly influence the degree of inhibition of PSII electron transport and the quenching of chlorophyll fluorescence . - pubmed
The collagen content and the ratio of collagenous to noncollagenous proteins ( hydroxyproline : proline ratio ) were determined by performing amino acid analysis on hydrolyzed samples using high - performance liquid chromatography . - pubmed
Collagen denaturation was quantified with a colorimetric assay by determining the amount of hydroxyproline released from samples treated with alpha - chymotrypsine . - pubmed
The hydroxyproline : proline ratio showed the same pattern as the collagen content . - pubmed
The percentage of collagen denaturation in the superficial layer was always significantly ( P & lt ; 0.01 ) higher than that in the deep layer , but no effect of the progesterone status or of the segment along the longitudinal axis was seen . - pubmed
Consistent with this , zeo1 - deficient cells suppress the growth defect of mutants in the Rho1p GDP - GTP exchange factor Rom2p , while exacerbating the growth defect of sac7delta mutants at 37 degrees C. In contrast , mid2delta mutants have opposing effects to zeo1delta mutants , being synthetically lethal with rom2delta , and suppressing an 18 degrees C growth defect of sac7delta , while overexpression of MID2 rescues a rom2delta 37 degrees C growth defect . - pubmed
[ Prolonged insulin coma ; therapeutic effect of ANP-235 ] . - pubmed
coli is a homodimer in solution with a specific activity of 3.8 U mg(-1 ) and K(m ) for UDP - Gal of 114 microM. - pubmed
cruzi UDP - Glc 4 '- epimerase is unable to inter - convert UDP - N - acetylglucosamine and UDP - N - acetylgalactosamine . - pubmed
cruzi initiates O - glycosylation of its abundant GPI - anchored surface mucins via GlcNAcalpha1 - O - Thr / Ser rather than the GalNAcalpha1 - O - Thr / Ser linkage that is common for mucins from many other eukaryotes . - pubmed
There was no convincing evidence of reaccumulation of iron in the grafted liver in HH ; however , 1 subject demonstrated increased serum ferritin concentration and grade 2 hepatic siderosis . - pubmed
The full - length cDNA consisted of a 1,561 - bp open reading frame encoding lacIIIb , a 499 - amino - acid protein and a 21 - amino - acid signal peptide . - pubmed
However , recent studies identify a distinct mode for PKCdelta activation involving tyrosine phosphorylation by Src family kinases . - pubmed
The tyrosine - phosphorylated form of PKCdelta ( which accumulates in the soluble fraction of cells exposed to oxidant stress ) displays lipid - independent kinase activity and is uniquely positioned to phosphorylate target substrates throughout the cell ( not just on lipid membranes ) . - pubmed
New thrombolytic agents , such as reteplase , tenecteplase or desmoteplase , offer pharmacokinetic and dynamic advantages over recombinant tissue plasminogen activator and have been or are currently being tested for safety and efficacy in clinical trials . - pubmed
C1s prefers Leu or Val at P(3 ) and Gly or Ala residues at P(2 ) . - pubmed
One ( 5C1 ) of the 13 mAb obtained was used as capture antibody at 5 mug / ml and biotin - labeled specific polyclonal antiserum at 0.63 microg / ml served for detection . - pubmed
The key features of this approach are ( 1 ) the utilization of a Japp - Klingemann condensation / Fischer cyclization to prepare cycloalkyl [ b ] indolones , ( 2 ) the asymmetric borane reduction of these heterocyclic ketones with ( S )- OAB to obtain enantiomerically pure alcohols , and ( 3 ) the stereoselective S ( N ) 2 - displacement of these indole alcohol substrates with a carbon nucleophile under Mitsunobu conditions to set the C1 or C3 tertiary carbon stereocenter . - pubmed
Two of the transporters , NaS1 and NaS2 , carry substrates such as sulfate , selenate and thiosulfate . - pubmed
The other members of the family ( NaDC1 , NaDC3 , and NaCT ) are transporters for di - and tri - carboxylates including succinate , citrate and alpha - ketoglutarate . - pubmed
This review also discusses new members of the SLC13 superfamily that exhibit both sodium - dependent and sodium - independent transport mechanisms . - pubmed
Apical membrane P2Y4 purinergic receptor controls K + secretion by strial marginal cell epithelium . - pubmed
BACKGROUND : It was previously shown that K + secretion by strial marginal cell epithelium is under the control of G - protein coupled receptors of the P2Y family in the apical membrane . - pubmed
Receptor activation by uracil nucleotides ( P2Y2 , P2Y4 or P2Y6 ) leads to a decrease in the electrogenic K + secretion . - pubmed
The present study was conducted to determine the subtype of the functional purinergic receptor in gerbil stria vascularis , to test if receptor activation leads to elevation of intracellular [ Ca2 + ] and to test if the response to these receptors undergoes desensitization . - pubmed
Activation caused a biphasic increase in intracellular [ Ca2 + ] that could be partially blocked by 2 - aminoethoxy - diphenyl borate ( 2 - APB ) , an inhibitor of the IP3 receptor and store - operated channels . - pubmed
The ineffectiveness of suramin at the concentration used was consistent with P2Y4 but not P2Y2 . - pubmed
CONCLUSION : The results support the conclusion that regulation of K + secretion across strial marginal cell epithelium occurs by P2Y4 receptors at the apical membrane . - pubmed
The apparent lack of desensitization of the response is consistent with two processes : a rapid - onset phosphorylation of KCNE1 channel subunit and a slower - onset of regulation by depletion of plasma membrane PIP2 . - pubmed
CP27 - 215 was 15N labeled for NMR studies and a 2D 1H-15N - HSQC spectrum confirmed the presence of a well - ordered structure and the monomeric form of the protein . - pubmed
We found that mutant Sso1 proteins with insertions or duplications show limited function , whereas replacement of as few as three amino acids preceding the transmembrane domain resulted in a nonfunctional SNARE in vivo . - pubmed
Viability is also maintained when two proline residues are inserted in the juxtamembrane of Sso1p , suggesting that helical continuity between the transmembrane domain and the core coiled - coil domain is not absolutely required . - pubmed
The inability of NR1 to assimilate nitrate appears to be due to the lack of an active nitrate reductase in these cells . - pubmed
DI-6 culture growth was inhibited by 70 % at a chlorate concentration of 30 micromolar , whereas growth of NR1 was stimulated by more than 60 % on the same medium and by 100 % at a chlorate concentration of 30 millimolar . - pubmed
The results suggest that the deletions of the N - glycans or other amino acid substitutions in the GP5 ectodomains of the mutants affect the immunogenicity of the neutralization epitope and the specificity of the Abs raised to it but not the sensitivity of the virions to Ab neutralization . - pubmed
Aliskiren is the first in a new class of potent , orally effective renin inhibitors . - pubmed
Aliskiren produces dose - dependent BP reduction with few side effects and constitutes a novel pharmacologic approach to renin - angiotensin - aldosterone inhibition . - pubmed
Bicarbonate - induced stomatal closure as well as H ( 2 ) O ( 2 ) production were restricted by exogenous catalase or diphenylene iodonium ( DPI , an inhibitor of NAD ( P ) H oxidase ) . - pubmed
In conclusion , the combination therapy with Alendronate and Alfacalcidol exhibited superiority in terms of BMD , overall fractures , rate of falls and back pain over either Alendronate in combination with plain vitamin D or Alfacalcidol alone . - pubmed
These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids , and apoptosis - inducing activity of the extract was mediated by caspase-3 activation in tumor cells . - pubmed
We found : 1 ) the phagocytic activity of hSP - A is reduced with increasing levels of in vivo SP - A carbonylation ; 2 ) in vitro ozone exposure of hSP - A decreases its function in a dose - dependent manner as well as its ability to enhance phagocytosis of either gram - negative or gram - positive bacteria ; 3 ) the activity of both SP - A1 and SP - A2 decreases in response to in vitro ozone exposure of proteins with SP - A2 being affected more than SP - A1 . - pubmed
We conclude that both in vivo and in vitro oxidative modifications of SP - A by carbonylation reduce its ability to enhance phagocytosis of bacteria and that the activity of SP - A2 is affected more by in vitro ozone - induced oxidation . - pubmed
A correlation was seen ( R2=.911 ) with FAS expression plotted against percentage change in 64Cu - ATSM uptake with C75 treatment . - pubmed
CONCLUSIONS : Although Cu - ATSM has clinical relevance in the PET imaging of hypoxia in many tumor types , its translation to the imaging of prostate cancer may be limited by the overexpression of FAS associated with prostatic malignancies . - pubmed
We found that protein S significantly reduced acetylated low - density lipoprotein ( AcLDL ) uptake and binding by macrophages and decreased the intracellular cholesteryl ester content . - pubmed
Soluble Mer protein or tyrosine kinase inhibitor herbimycin A effectively blocked the effects of protein S on AcLDL uptake . - pubmed
Furthermore , the hydroxy substitutions in the positions C3 ( C - ring ) in C5 and C7 ( A - ring ) would be important for neuroprotection in this model . - pubmed
RATIONALE : Little is known about the incidence of isoniazid - associated hepatitis in HIV - infected Africans who receive both isoniazid preventive therapy ( IPT ) and antiretroviral therapy ( ART ) . - pubmed
INH - associated hepatitis ( INH - hepatitis ) was defined as having either alanine or aspartate aminotransferase greater than 5.0 times the upper limit of normal regardless of symptoms when INH was not excluded as the cause . - pubmed
Antiretroviral therapy ( ART ) was received by 480 participants but was not statistically associated with INH - hepatitis ( relative risk [ RR ] , 1.56 ; 95 % confidence intervals [ CI ] , 0.62 - 3.9 ) ; those receiving nevirapine had a higher rate ( 2.0 % ) than those receiving efavirenz ( 0.9 % ; P = 0.34 ) . - pubmed
PTH - level reduction , which was significant versus baseline in both groups , although not statistically different between groups , was higher with cinacalcet . - pubmed
We investigated the effects of angiotensin II ( ANG II)-dependent hypertension on the platelet proteome . - pubmed
Inhibition of hepatitis C virus NS3 helicase by manoalide . - pubmed
Reaction pathway and free energy profile determined for specific recognition of oligosaccharide moiety of carboxypeptidase Y. - pubmed
lectin , which interacts with the carbohydrate moiety on a similar way . - pubmed
No post fixation was necessary for ICC , whereas , 20 minutes post fixation in formalin ( 4 % ) was necessary for ISH . - pubmed
Post fixation in formalin is necessary for ISH . - pubmed
BMPs signal via transmembrane type I and type II serine / threonine kinase receptors and intracellular Smad effector proteins . - pubmed
Serum androgens , lipids , insulin resistance and glucose metabolism measures were also determined . - pubmed
, thiophene ( T )- thienopyrazine ( TP ) , the electronic properties vary nonlinearly with D / A compositions . - pubmed
[ Successful treatment of trastuzumab - resistant HER2 - positive breast cancer with extensive liver metastases using the combination of trastuzumab and capecitabine - a case report ] . - pubmed
RESULTS : In PD population , there were no statistical relationships between early and delayed ( 123)I MIBG cardiac and ( 123 ) I FP - CIT striatal uptake in contralateral caudate ( P > 0.05 ) and in contralateral putamen ( P > 0.05 ) to the side mainly affected ; no statistically significant relationships have been found at any level when considering ipsilateral striatum . - pubmed
All three mast cell populations were rendered hyporesponsive by ES-62 and whilst the mechanisms underlying such desensitisation have not been fully delineated , they reflect a downregulation of calcium and PKC alpha signalling . - pubmed
coli O157 : H7 , over - expression of PDE decreased c - di - GMP level . - pubmed
We further observed decreased curli fimbriae synthesis in the strain with decreased c - di - GMP levels , which was supported by the reduction in the transcription of curli large subunit gene csgA and the curli expression regulator gene csgD. - pubmed
In fact , two histone deacetylase inhibitors ( HDIs ) , SAHA and Depsipeptide , are FDA approved for single - agent treatment of refractory cutaneous T cell lymphoma ( CTCL ) . - pubmed
Evaluation of C - reactive protein before and on - treatment as a predictor of benefit of atorvastatin : a cohort analysis from the Anglo - Scandinavian Cardiac Outcomes Trial lipid - lowering arm . - pubmed
OBJECTIVES : The aim of this study was to determine whether baseline and on - statin C - reactive protein ( CRP ) are independent predictors of cardiovascular ( CV ) outcome beyond low - density lipoprotein cholesterol ( LDL - C ) . - pubmed
After 6 months of atorvastatin therapy , the median LDL - C and CRP were reduced by 38.7 % and 25.8 % , respectively . - pubmed
In the circulation 25 - hydroxyvitamin D ( 25 ( OH ) D ) is bound to vitamin D - binding protein ( DBP ) and albumin . - pubmed
Serum 25 ( OH ) D , DBP and albumin were measured and the free and bio - available ( free + albumin - bound ) 25 ( OH ) D calculated . - pubmed
Based on genotyping of the polymorphisms rs7041 and rs4588 , the six common DBP phenotypes were identified and the free and bio - available 25 ( OH ) D calculated according to the corresponding binding coefficients . - pubmed
Relations between measures of 25 ( OH ) D and PTH and BMD were evaluated with linear regression adjusted for age and BMI . - pubmed
Adjusting for DBP phenotype affected the calculated free and bio - available 25 ( OH ) D levels up to 37.5 % . - pubmed
All measures of 25 ( OH ) D correlated significantly with PTH , whereas a significant association with BMD was only seen for the free and bio - available 25 ( OH ) D measures . - pubmed
A role beyond learning for NMDA receptors in reward - based decision - making - a pharmacological study using d - cycloserine . - pubmed
Immune cells , stimulated by NEFAs , produce pro - inflammatory cytokines , which have a direct effect on oxidating radicals that directly target the release of noradrenalin . - pubmed
Cement distribution ( 2 ml PMMA ) was analyzed on CT . - pubmed
Mutations in the KCNJ13 gene that encodes the inwardly rectifying potassium channel Kir7.1 cause snowflake vitreoretinal degeneration ( SVD ) and leber congenital amaurosis ( LCA ) . - pubmed
Kir7.1 controls the microenvironment between the photoreceptors and the retinal pigment epithelium ( RPE ) and also contributes to the function of other organs such as uterus and brain . - pubmed
We have determined that the mutation results in a severe truncation of the Kir7.1 C - terminus , alters protein localization , and disrupts potassium currents . - pubmed
RPTPsigma is reciprocally regulated by interactions with chondroitin sulfate or heparan sulfate containing extracellular proteoglycans in a mechanism called the proteoglycan switch . - pubmed
Osteoconductivity on these scaffolds was investigated by the common osteogenic markers alkaline phosphatase ( ALP ) activity , calcium mineral deposition and bone - related gene activation . - pubmed
CONCLUSIONS : Osteoconductivity of PES nanofibres was markedly enhanced after coating them with BG , and introduction of this construct as new bone - graft substitute for bone loss and defects is indicated . - pubmed
Incubation of bone chips with 0.2 % chlorhexidine , followed by vigorously washing resulted in a BCM with even higher expression of IL11 , PRG4 and NOX4 . - pubmed
Chlorhexidine alone , at low concentrations , increased IL11 , PRG4 and NOX4 expression , independent of the TGF - beta receptor I kinase activity . - pubmed
Quantitative hepatitis B surface antigen combined with hepatitis B e antigen as sustained virological response predictors during extended therapy with Peginterferon alfa-2a for hepatitis B e antigen - positive chronic hepatitis B. - pubmed
The serum HBsAg cut - off of 1800IU / mL at week 48 had area under curve ( AUC ) of 0.727 , and the serum HBsAg cut - off of 1500IU / mL , combined with HBeAg loss at week 72 , had AUC of 0.753 to predict SVR during the follow - up . - pubmed
The results in our study provide the first experimental evidence that both caspase - dependent and AIF - dependent apoptosis pathways are involved in the loss of the nigral DA neurons caused by intrastriatal administration of 6 - OHDA , and intrastriatal preadministration of Bip - V5 can inhibit the above two apoptosis pathways to protect the nigral DA neurons . - pubmed
The hormone islet amyloid polypeptide ( IAPP , or amylin ) plays a role in glucose homeostasis but aggregates to form islet amyloid in type-2 diabetes . - pubmed
Activation of the inflammasome , defects in autophagy , ER stress , generation of reactive oxygen species , membrane disruption , and receptor mediated mechanisms have all been proposed to play a role . - pubmed
We engineered an aTF , the Escherichia coli lac repressor , LacI , to respond to one of four new inducer molecules : fucose , gentiobiose , lactitol and sucralose . - pubmed
Using computational protein design , single - residue saturation mutagenesis or random mutagenesis , along with multiplex assembly , we identified new variants comparable in specificity and induction to wild - type LacI with its inducer , isopropyl beta - D-1 - thiogalactopyranoside ( IPTG ) . - pubmed
This article highlights the different types of images that can be generated by using dual - energy CT protocols such as virtual monochromatic , virtual unenhanced ( ie , water ) , and pulmonary blood volume ( ie , iodine ) images . - pubmed
Elevated UCP2 levels also protected against excitotoxic cell death induced by intraocular injection of either NMDA or kainic acid . - pubmed
In vitro release study of PL - DOC showed that PL - DOC had a sustained release effect . - pubmed
Non - nucleoside reverse transcriptase inhibitor ( NNRTI ) resistance was detected in 17 of 26 ( 65 % ) patients , 2 ( 7 % ) had Thymidine analogue mutations , and 3 ( 11 % ) had K65R. - pubmed
Recent work has established that , in the brain and other systems , the GR is translocated from the cytosol to the mitochondria and that stress and corticosteroids have a direct influence on mtDNA transcription and mitochondrial physiology . - pubmed
ChIP sequencing of GR showed that corticosterone treatment induced a dose - dependent association of the GR with the control region of the mitochondrial genome . - pubmed
Moreover , 19 protein bands from SDS - PAGE were analyzed and a total of 45 different proteins were identified by LC - MS / MS . - pubmed
PARP inhibitor 4 - aminobenzamide ( 4 - AB ) was administered daily for ten weeks after diabetes induction . - pubmed
PARP-1 activity , reduced glutathione ( GSH ) and nitrite contents were assessed in heart muscle . - pubmed
Fasting glucose , fructosamine , insulin , and tumor necrosis factor alpha ( TNF - alpha ) levels were measured in serum . - pubmed
The pore size distributions calculated using the nitrogen adsorption isotherms and TEM images show that all carbons are mainly nanoporous with certain contribution of narrow mesopores ( at pore half - width x & lt ; 5 nm ) . - pubmed
Mo polyoxometalate nanoparticles inhibit tumor growth and vascular endothelial growth factor induced angiogenesis . - pubmed
Furthermore , western blot analysis of cell signaling molecules indicated that Mo POMs blocked the vascular endothelial growth factor receptor 2 - mediated ERK1 / 2 and AKT signaling pathways in endothelial cells . - pubmed
One day post - SAH , brain tissue damage was measured with T2 - weighted magnetic resonance imaging , followed by cortical application of 1 mol / L KCl ( to induce SDs ) or NaCl ( no SDs ) . - pubmed
In mESCs , acetyl - LIFR undergoes homodimerization , leading to STAT3 hypo- or hyper - activation depending on the presence or absence of gp130 . - pubmed
Thus , LIFR acetylation and serine phosphorylation differentially promote stem cell self - renewal and differentiation . - pubmed
Synucleinopathies are neurodegenerative diseases characterized by the abnormal accumulation of alpha - synuclein ( alpha - Syn , a 140 amino acid protein that normally plays various neurophysiologic roles ) aggregates . - pubmed
However , under ABA , it showed higher levels of both peroxidases . - pubmed
Morphology of skin tissue was observed with HE staining ; tumor necrosis factor - alpha ( TNF - alpha ) content in serum and skin tissue was determined by enzyme - linked immunosorbent assay ; superoxide dismutase ( SOD ) content in serum and skin tissue was determined by hydroxylamine method ; malondialdehyde content in serum and skin tissue was determined by thiobarbituric acid method . - pubmed
CONCLUSIONS : The ultimate failure load was significantly greater in the F - MMA than the TSS group and similar to the conventional MMA technique . - pubmed
Furthermore , the proposed method was successfully applied to detect Co ( II ) in living cell samples , and the results were agreed with those obtained by the standard ICP - MS method . - pubmed
We also show that SAA could increase the chemotherapeutic efficacy of cisplatin , suggesting a synergistic effect of SAA and cisplatin . - pubmed
Thyroglobulin antibodies ( TG - aAb ) , thyroid peroxidase antibodies ( TPO - aAb ) , TSH , free T3 , free T4 , total T3 , and total T4 , as well as urine iodine excretion were determined . - pubmed
Se status was assessed by serum Se and selenoprotein P ( SELENOP ) concentrations . - pubmed
RESULTS : Serum Se and SELENOP concentrations were relatively low in both control and AITD women . - pubmed
The decline in TPO- and TG - aAb was unrelated to the Se status . - pubmed
Conditions that favor the helical structure formation in structurally similar sophorolipids ( SLs ) , that is , elaidic acid SLs ( having a trans double bond between the C9 and C10 positions of the alkyl chain ) and stearic acid SLs ( no double bond ) , are presented here . - pubmed
Despite new studies comparing insulin pump therapy to multiple daily injections , its effectiveness in improving glucose and pregnancy outcomes remains unclear . - pubmed
The long - term course of PRP symptoms was also examined , along with current ( today ) levels of trait anxieties . - pubmed
By matching the redox capacity of the PTMA- co - BP thin film and bis ( alkoxy )- substituted poly ( propylenedioxythiophene ) , the ECD achieved an optical contrast of 72 % in a small potential window of 2.55 V ( i.e. - pubmed
Novel approach to quorum quenching : rational design of antibacterials in combination with hexahistidine - tagged organophosphorus hydrolase . - pubmed
In this article the lactonase activity of the preparations based on hexahistidine - tagged organophosphorus hydrolase ( His6 - OPH ) towards AHLs was studied . - pubmed
Initially , three of the most interesting beta - lactam antibiotics were selected from seven that were trialed during molecular docking to His6 - OPH . - pubmed
The lactonase activity of the preparations was investigated under different physical - chemical conditions in the hydrolysis of AHLs [ N - butyryl - D , L - homoserine lactone , N -( 3 - oxooctanoyl )- D , L - homoserine lactone , N -( 3 - oxododecanoyl )- L - homoserine lactone ] . - pubmed
The broadening of the catalytic action of the enzyme against AHLs was revealed in the presence of the meropenem . - pubmed
Results of molecular docking of AHLs to the surface of the His6 - OPH dimer in the presence of antibiotics allowed proposing the mechanism of such interference based on a steric repulsion of the carbon chain of hydrolyzed AHLs by the antibiotics bounded to the enzyme surface . - pubmed
Cisplatin ( CP ) is one of the most potent anti - cancer drugs used against different types of cancer . - pubmed
Urine and blood samples were collected for the evaluation of kidney function including serum creatinine , BUN , cystatin - c , and creatinine clearance . - pubmed
We noticed that both doses of tempol significantly improved kidney function , which was deteriorated by CP injection . - pubmed
Tempol significantly elevated kidney glutathione ( GSH ) content and SOD activity , and decreased kidney lipid peroxidation and NOx production . - pubmed
Tempol also significantly decreased kidney caspase-3 expression which was elevated by CP toxicity . - pubmed
Thus , we conclude that tempol can protect against CP nephrotoxicity . - pubmed
We noticed that both doses of tempol are effective in ameliorating CP - nephrotoxicity . - pubmed
Treatment with LO for 8 weeks induces a mean reduction of 13 C - ALA conversion to 13 C - DHA by 48 % as reflected by the LDL compartment , and a mean reduction of the 13 C - ALA incorporation into LDL by 46 % . - pubmed
CONCLUSION : A 2 - month dietary intake of a high dose of LO is sufficient to reach saturation of ALA incorporation into LDL particles , which are responsible for ALA distribution in the body . - pubmed
Inhibition of nitric oxide ( NO ) production significantly increased the sensitivity of cells from the normal pregnancies to TNFalpha but not in the high risk group establishing a functional role for NO in this system . - pubmed
[ Indoleamine 2,3 - Dioxygenase Activity during Long - Term Letrozole Therapy for Hormone Receptor - Positive Breast Cancer ] . - pubmed
We evaluated the significance of indoleamine 2,3 - dioxygenase(IDO)during letrozole therapy in long - term response . - pubmed
We measured IDO activity based on the tryptophan(Trp)/kynurenine(Kyn)ratio . - pubmed
Recently identified imidazoline receptors of the first type ( I1Rs ) on the cardiomyocyte 's sarcolemma open a new field in calcium signaling research . - pubmed
Here we investigated the effects of I1Rs activation on L - type voltage - gated Ca2 + -currents under catecholaminergic stress induced by the application of beta - agonist , isoproterenol . - pubmed
Pharmacological agonist of I1Rs ( I1 - agonist ) , rilmenidine , and the putative endogenous I1 - ligand , agmatine , have been shown to effectively reduce Ca2 + -currents potentiated by isoproterenol . - pubmed
Inhibitory analysis shows that the ability to suppress voltage - gated Ca2 + -currents by rilmenidine and agmatine is fully preserved in the presence of the protein kinase A blocker ( PKA ) , which indicates a PKA - independent mechanism of their action . - pubmed
The blockade of NO synthase isoforms with 7NI does not affect the intrinsic effects of agmatine and rilmenidine , which suggests NO - independent signaling pathways triggered by I1Rs . - pubmed
A nonspecific serine / threonine protein phosphatase ( STPP ) inhibitor , calyculin A , abrogates effects of rilmenidine or agmatine on the isoproterenol - induced Ca2 + -currents . - pubmed
TAT peptide - modified cisplatin - loaded iron oxide nanoparticles for reversing cisplatin - resistant nasopharyngeal carcinoma . - pubmed
On this basis , modification with TAT peptide ( YGRKKRRQRRR ) significantly improved tumor intracellular uptake , devoting to better curative effects and minimized side effects by reducing CDDP therapeutic doses . - pubmed
The sandwich fragments are eta2 - coordinated to the second metal center with the average M - C distances of 2.837(4 ) A and 2.879(5 ) A for yttrium and lanthanum complexes , respectively . - pubmed
Investigations on the Relationship between Ovarian , Endocrine , and Renal Findings in Nonclinical Safety Studies of the gamma - secretase Inhibitor Avagacestat . - pubmed
Of note , these pro- or anti - EndMT effects were reversed by the EP4 overexpression or the EP4 antagonist AH-23848 , respectively . - pubmed
Polymorphisms in CEP72 have been linked to increased incidence of vincristine - induced toxicities , namely peripheral neuropathy . - pubmed
Hepatic expression of genes involved in inflammation , as well as glucose and lipid metabolism , were determined by quantitative polymerase chain reaction . - pubmed
During the PAP , EKD showed a more pronounced increase in glucose levels at the first hour after LPS challenge compared with the CD - LPS group . - pubmed
The Influence of Glycans - Specific Bioconjugation on the FcgammaRI Binding and In vivo Performance of 89Zr - DFO - Pertuzumab . - pubmed
To circumvent this issue , we have previously developed a chemoenzymatic bioconjugation strategy that site - specifically appends cargoes to the biantennary heavy chain glycans attached to CH2 domains of the immunoglobulin 's Fc region . - pubmed
Methods : We synthesized three desferrioxamine ( DFO)-labeled immunoconjugates based on the HER2 - targeting antibody pertuzumab : one using random bioconjugation methods ( DFO - nsspertuzumab ) and two using variants of our chemoenzymatic protocol ( DFO - sspertuzumab - EndoS and DFO - sspertuzumab - betaGal ) . - pubmed
Results : MALDI - ToF and SDS - PAGE analysis confirmed the site - specific nature of the bioconjugation , and flow cytometry and surface plasmon resonance ( SPR ) revealed that all three immunoconjugates bind HER2 as effectively as native pertuzumab . - pubmed
Critically , however , SPR experiments also illuminated that DFO - sspertuzumab - EndoS possesses an attenuated binding affinity for huFcgammaRI ( 17.4 + - 0.3 nM ) compared to native pertuzumab ( 4.7 + - 0.2 nM ) , DFO - nsspertuzumab ( 4.1 + - 0.1 nM ) , and DFO - sspertuzumab - betaGal ( 4.7 + - 0.2 nM ) . - pubmed
Neutralization of lysosomal pH effectively reduced ZVI@Ag NP - induced programmed cell death . - pubmed
Our results revealed the successful preparation of AE - loaded folate - targeted nanoparticles ( NP - F - AE ) with particle size around 177 nm , negative zeta potential , polydispersity index < 0.20 , and higher efficiency of encapsulation for both GAs present in the extract ( > 85 % ) . - pubmed
To investigate the cellular uptake , the fluorescent dye coumarin-6 was encapsulated into the nanoparticle ( NP - F - C6 ) . - pubmed
The study found that EX-527 reversed OVA - induced airway inflammation , and reduced OVA - induced increases in inflammatory cytokine expression , and total cell and eosinophil counts in bronchoalveolar lavage fluid . - pubmed
All effects induced by EX-527 , including increased phosphorylated - mTOR and decreased autophagy , were abrogated by rapamycin treatment . - pubmed
Apoptotic and antihepatofibrotic effect of honokiol via activation of GSK3beta and suppression of Wnt / beta - catenin pathway in hepatic stellate cells . - pubmed
Honokiol increased sub G1 population and activated caspase 3 and cleaved poly ( ADP - ribose ) polymerase ( PARP ) in HSCs . - pubmed
Moreover , honokiol attenuated the expression of alpha smooth muscle actin ( alpha - SMA ) , transforming growth factor beta 1 ( TGF - beta1 ) , phospho - Smad3 , phospho - AKT , cyclin D1 , c - Myc , Wnt3a , beta - catenin , and activated phosphorylation of glycogen synthase kinase 3 beta ( GSK3beta ) in HSCs . - pubmed
Overall , honokiol exerts apoptotic and antifibrotic effects via activation of GSK3beta and inhibition of Wnt3a / beta - catenin signalling pathway . - pubmed
NSUN2 is an RNA methyltransferase responsible for the m5C modification of multiple RNAs . - pubmed
Mechanistically , we determined that Pkm2 interacts with beta - catenin ( Ctnnb1 ) in the cytoplasm of CM , inhibiting Ctnnb1 phosphorylation at serine 552 and tyrosine 333 , by Akt . - pubmed
The accumulation of unrepaired ribonucleotides is associated with genomic instability , which is mediated by DNA topoisomerase 1 ( Top1 ) processing of embedded ribonucleotides . - pubmed
The cleavage initiated by Top1 at the site of a ribonucleotide leads to the formation of a Top1 - DNA cleavage complex ( Top1cc ) , occasionally resulting in a DNA double - strand break ( DSB ) . - pubmed
BACKGROUND AND AIMS : Cholangiocytes express cystic fibrosis transmembrane conductance regulator ( CFTR ) which is involved in bicarbonate secretion for the protection against bile toxicity . - pubmed
Thus , the aim of this study is to investigate the effect of hypoxia on CFTR activity in intrahepatic cholangiocyte organoids ( ICOs ) and test drug - interventions to restore bicarbonate secretion . - pubmed
Under oxygenated conditions , both CFTR and ANO1 channels were responsible for forskolin and UTP activated anion - secretion . - pubmed
Forskolin - stimulation in absence of intracellular chloride showed ion - transport , indicating that bicarbonate could be secreted by CFTR . - pubmed
Switching from oxygen to hypoxia during CFTR - measurements , reduced CFTR activity ( p=0.03 ) . - pubmed
Cholesterol depletion , which was achieved by a mild physiological treatment , and which increased the membrane - lipid fluidity , resulted in a substantial increase in the average number of the available transferrin receptors per bone marrow cell . - pubmed
Response of plasma insulin to small doses of tolbutamide in obesity and acromegaly . - pubmed
In acromegaly the insulin release pattern is compatible with an increased number of secretory units which , however , are relatively insensitive to tolbutamide , probably due to an elevated serum growth hormone level . - pubmed
In addition , secondary B cells can be stimulated in collaboration with allogeneic antigen - specific T cells to yield clones producing antibody of the IgG1 isotope , whereas primary B cells similarly stimulated produce only IgM antibodies . - pubmed
She had undergone R2 curative subtotal gastrectomy in June 1985 , and had orally taken tegafur 600 mg / day and then changed to doxifluridine 800 mg / day as postoperative adjuvant chemotherapy . - pubmed
Five months after the operation she had back pains and both 99mTc - MDP and 67Ga - citrate scintigram showed L1 vertebra and rib bone metastasis . - pubmed
However , at 0.1 - 1.0 microM , verapamil prevented or attenuated prolongation of transmural CT by ischemic conditions and reperfusion . - pubmed
Transmural CT was further prolonged at 3 microM verapamil . - pubmed
The inhibitory activity of CD4 + Leu-8 + T cell supernatants could not be accounted for by a deficiency or excess of IL-2 , IL-4 , IFN - gamma , IL-6 , or PGE2 . - pubmed
A Radial - Pak microBondapak C18 reversed - phase cartridge was used for the separations and elutions were carried out with acetonitrile - water - methanol - acetic acid mixtures . - pubmed
rpoH111 was a point mutation resulting in a single amino acid substitution . - pubmed
In common with other heavy SR preparations , it contains predominantly the calcium pump membrane ( 80 - 85 % of the membrane ) and compartmental contents ( CC ) , consisting mainly of calcium - binding protein ( calsequestrin ) . - pubmed
Effects of disodium ethane-1 - hydroxy-1,1 - diphosphonate ( EHDP ) on interleukin 1 production by macrophages . - pubmed
A high dose ( 1 mg / ml ) of EHDP inhibited interleukin 1 ( IL 1 ) production by oil - induced peritoneal macrophages stimulated with muramyl dipeptide ( MDP ) , lipopolysaccharide ( LPS ) , phorbol myristic acetate ( PMA ) , heat - aggregated IgG2 or calcium ionophore A23187 . - pubmed
On the other hand , low doses ( less than 0.125 mg / ml ) of EHDP augmented the MDP induced IL 1 production by macrophages . - pubmed
This biphasic effect was also observed when macrophages were exposed to EHDP at 37 C for 24 hr and then stimulated with IL 1 inducers . - pubmed
These results demonstrated that EHDP acted on macrophages directly and modulated IL 1 production in vitro . - pubmed
[ Hormone receptor determination in breast cancer using the dextran- coated charcoal method and monoclonal antibodies : correlation with cytomorphologic grading ] . - pubmed
In this study , cells derived from primitive neuroectodermal tumors of the posterior fossa ( PNET - PF ) were examined for their sensitivity to LAK cytolysis utilizing an in vitro 4 - hour chromium-51 - release assay . - pubmed
Flow cytometric measurements of Ca antigen ( Ca ) visualized by immunofluorescence with Ca1 monoclonal antibody ( Ca1 ) were performed on cells from 26 urinary bladder carcinomas of various histologic grades and DNA ploidy and on 6 samples of morphologically normal urothelium . - pubmed
Chromatography is performed on a reversed - phase C18 column , connected with a fixed - wavelength ultraviolet detector at 280 nm ; the compounds are eluted in about 5 min with tetrahydrofuran - acetonitrile-0.01 M sodium phosphate buffer , pH 7.4 ( 12:25:63 , v / v / v ) . - pubmed
Inactivation of melanocyte - stimulating hormone release - inhibiting factor by a manganese - stimulated bovine brain aminopeptidase . - pubmed
Although mitotic rate was subnormal , several other factors attest to the essential viability of the cultured intestine : L - leucine incorporation into protein , as well as the synthesis of a specific vitamin D ( 3 )-induced calcium - binding protein ( CaBP ) , increased over a 48 - h culture period , and the electropotential gradient across the intestine was maintained throughout the culture period as was a concentration gradient for calcium . - pubmed
The tissue responded to vitamin D ( 3 ) in the medium by synthesizing the calcium - binding protein within 6 h and by exhibiting enhanced ( 45)Ca uptake within 12 - 24 h. Concentrations of vitamin D ( 3 ) , or its 25 - hydroxylated derivative , higher than necessary for CaBP induction , also increased the activity of alkaline phosphatase . - pubmed
The 1,25 - dihydroxylated derivative of vitamin D ( 3 ) , at a level extremely potent in CaBP induction , did not stimulate alkaline phosphatase . - pubmed
Other embryonic organs , esophagus , stomach , liver , pancreas , lung , skin , and muscle , did not produce CaBP in response to vitamin D ( 3 ) in the culture medium . - pubmed
Fine - structural localization of acid phosphatase has been studied in triparanol - treated adrenal glands . - pubmed
Lead phosphate reaction product ( indicating acid phosphatase activity ) is demonstrable within these membrane - limited cytoplasmic bodies , identifying them as morphological lysosomes . - pubmed
Urinary kallikrein activity , renal hemodynamics , and electrolyte handling during chronic beta blockade with propranolol in hypertension . - pubmed
In the in vitro tests , both groups of agonists inhibited electrically evoked activity ; however in these experiments only antagonism of opiates by naloxone and alpha 2 - agonists by yohimbine could be shown and it is concluded that in these systems the activity of the alpha 2 - agonists is mediated via the presynaptic alpha 2 - receptors only . - pubmed
Experiments have been carried out to provide direct evidence for the proposed presynaptic 5 - HT autoreceptor agonist activity of 8 - hydroxy-2 -( di - n - propylamino ) tetralin ( 8 - OH - DPAT ) a compound with selectivity for the 5 - HT1A subtype of the 5 - HT1 binding site . - pubmed
These experiments have therefore failed to provide direct evidence for agonist activity of 8 - OH - DPAT at the 5 - HT autoreceptor and alternative explanations must be sought for its biochemical and behavioural effects in vivo . - pubmed
Moreover , the fact that 8 - OH - DPAT is inactive at the autoreceptor at concentrations selective for the 5 - HT1A recognition site suggests that this subtype of the 5 - HT1 binding site may not correspond to the 5 - HT autoreceptor . - pubmed
Plasma renin substrate concentration during chronic propranolol therapy . - pubmed
GTP - insensitive ornithine decarboxylase in acetobacteria able to synthesize spermine . - pubmed
However , their S - adenosyl - L - methionine decarboxylase resembled other prokaryotic adenosylmethionine decarboxylases in requiring Mg2 + ions in vitro for full activity . - pubmed
Plasma cholesterol levels rose sharply in the HCO and SFO groups but not at all in the EPO group . - pubmed
EPO contains the product of delta-6 - desaturation , 18:3n-6 , suggesting that conversion of linoleic acid to 18:3n-6 and possibly to further metabolites may be important for the cholesterol - lowering effect of polyunsaturates . - pubmed
These findings together with our results which show inhibition of AR by tyrosine - phosphorylation inhibitors , indicate that ejaculated bull sperm contain a typical 170 - kDa EGFR which is active in the mechanism leading to AR . - pubmed
This selection consisted of the absence in vitro of genomes with certain amino - acid substitutions at or near conserved glycosylation sites of the C2 region at positions 276 and 289 . - pubmed
Quantitative autoradiography was used to assess alterations in serotonin ( 5 - HT ) receptor binding in the hypothalamus and hippocampus following denervation with 5,7 - dihydroxytryptamine ( 5,7 - DHT ) , ovariectomy ( OVX ) and gonadal steroid manipulation . - pubmed
In addition , there was a significant interaction between OVX and 5,7 - DHT for both the 5 - HT1a receptor and the 5 - HT transporter in the VMN , such that OVX inhibited the 5,7 - DHT - induced increase in 5 - HT1a receptors and attenuated the 5,7 - DHT - induced decrease in 5 - HT transporter binding . - pubmed
In a separate experiment the effect of gonadal steroid manipulation on 5 - HT receptor binding was assessed . - pubmed
Subtype - specific binding of azidoanilido - GTP by purified G protein alpha subunits . - pubmed
Azidoanilido - GTP ( AA - GTP ) , a hydrolysis - resistant , photoreactive GTP analog , is becoming an increasingly popular tool for identifying activation of specific G proteins by receptors within native plasma membranes . - pubmed
Despite the use of AA - GTP as an affinity probe , surprisingly little is known regarding the ability of various G protein alpha subunits to bind this analog . - pubmed
To directly address this issue , we compared the ability of four purified G protein alpha subunits ( Go , Gi2 , Gs , and Gz ) to bind AA - GTP with their ability to bind GTP gamma S , a GTP analog commonly used to characterize the GTP - binding properties of G proteins . - pubmed
All four G alpha subunits tested bound AA - GTP in a manner distinct from their binding of GTP gamma S. - pubmed
One of these proteins , Gs alpha , required millimolar levels of free Mg2 + for significant binding of AA - GTP , while Go alpha and Gi alpha 2 displayed peak AA - GTP binding at approximately 100 microM free Mg2 + . - pubmed
The fourth G alpha subunit , Gz , bound AA - GTP very poorly relative to GTP gamma S regardless of the magnesium concentration . - pubmed
These results indicate that individual G protein alpha subunits differ markedly in their ability to bind AA - GTP . - pubmed
Use of AA - GTP to identify specific G protein - receptor interactions must therefore take into account the varied abilities of G alpha subunits to bind this analog . - pubmed
Bovine serum albumin ( BSA ) is used as acceptor of free fatty acids in the incubation medium of isolated fat cells . - pubmed
The assay involves pretreatment with the detergent sodium dodecyl sulfate ( SDS ) to liberate the free fatty acids from the bovine serum albumin before activation by acyl - CoA synthetase ( ACS ) ( EC 6.2.1.3 ) . - pubmed
This is followed by oxidation of the resulting thioesters by acyl - CoA oxidase ( ACO ) . - pubmed
The H2O2 formed is subsequently measured in a horseradish peroxidase ( HRP ) ( EC 1.11.1.7)-catalyzed luminol reaction . - pubmed
No interference was observed up to the following concentrations : 5 microM epinephrine , 5 microM norepinephrine , 80 microM isoproterenol , 1 mM insulin , 2.5 mM propranolol , 5 mM phentolamine , and 5 microM ascorbate . - pubmed
Associations with serum concentrations of total , low - density lipoprotein ( LDL ) , high - density lipoprotein-2 ( HDL2 ) , and HDL3 cholesterol , apolipoproteins ( apos ) A - I and B , lipoprotein ( a ) [ Lp ( a ) ] were studied . - pubmed
Serum total and free testosterone concentrations were positively correlated with HDL and HDL2 cholesterol and apo A - I. - pubmed
Serum total and free estradiol levels showed borderline - significant negative associations with total and LDL cholesterol levels . - pubmed
Among urinary variables , total estradiol excretion was negatively associated with apo B levels and showed borderline - significant associations with total and LDL cholesterol . - pubmed
Adjustment for potential confounders , including age , body mass index ( BMI ) , and waist to hip ratio ( WHR ) , strengthened the associations between urinary total estradiol and serum total cholesterol , LDL cholesterol , and apo B. - pubmed
Urinary free estradiol showed a significant correlation with HDL3 cholesterol . - pubmed
Urinary excretion of total testosterone was significantly negatively associated with serum cholesterol and LDL cholesterol levels only after controlling for confounding variables . - pubmed
HoloPYP could be reconstituted by the addition of p - coumaric anhydride to the histidine - tagged apoPYP ( PYP lacking its chromophore ) . - pubmed
No association found between Alzheimer ' s disease and a mitochondrial tRNA glutamine gene variant . - pubmed
In contrast , purified NS3 in which the predicted catalytic serine has been mutated loses protease but retains ATPase activity . - pubmed
Our results support the concept that HLAL and HGL / RLL are members of a gene family of lipases that most likely have evolved by duplication of an ancestral gene and subsequently assumed distinct roles in neutral lipid metabolism due to sequence divergence and different expression patterns . - pubmed
When control PMNs were tested , active UC rectal dialysate was almost as effective ( +115 % ) as N - formyl - methionyl - leucyl - phenylalanine ( +132 % ) in up - regulating CD11b . - pubmed
Changes in intracellular calcium levels paralleled these changes in CD11b . - pubmed
Rectal dialysate - induced changes in both CD11b and calcium paralleled our previous findings on rectal dialysate - induced changes in ROS production . - pubmed
Exposure of macrophages to TGF - beta1 led to a rapid and sustained increase in uPA mRNA levels , which was independent of de novo protein synthesis and completely inhibited by actinomycin D. - pubmed
Likewise , okadaic acid potentiated the ability of TGF - beta to up - regulate macrophage uPA expression . - pubmed
One copy of MeIARV was found to be inserted at the end of the 6th leucine domain of the c - maf proto - oncogene , which encodes a basic region / leucine zipper transcription factor related to the AP-1 family that is able to form homodimers or heterodimers with Fos and Jun transcription factors . - pubmed
Nitric oxide - induced downregulation of Cdk2 activity and cyclin A gene transcription in vascular smooth muscle cells . - pubmed
The addition of SNP or SNAP to VSMC cultures at the time of serum stimulation abrogates the induction of cdk2 activity without suppressing protein levels of cdk2 or cyclin E. - pubmed
These NO donors block serum - stimulated upregulation of cyclin A mRNA and protein and repress the serum induction of cyclin A promoter activity in VSMCs . - pubmed
CONCLUSIONS : The addition of the nitric oxide donors SNP or SNAP to mitogen - stimulated VSMCs prevents activation of cdk2 , a key regulator of the G1 and S phases of the cell cycle . - pubmed
These NO donors do not affect the expression of cdk2 protein but block the mitogen - induced expression of cyclin A , an activating subunit of cdk2 . - pubmed
SNP and SNAP also repress the mitogen - stimulated activation of the cyclin A promoter . - pubmed
These data suggest that the antiproliferative effect of NO on VSMCs results , at least in part , from the repression of cyclin A gene transcription . - pubmed
Overall , the findings from the present study provide additional evidence that there is basolateral uptake of inorganic mercury in the kidneys and that the primary mechanism involved in this basolateral uptake is dependent on the activity of the organic anion transporter . - pubmed
The dose - limiting toxicity ( DLT ) of UFT has generally been diarrhea . - pubmed
Enzyme solubilization by digitonin , affinity chromatography purification and ultrafiltration underlie this method . - pubmed
Characterization of the folding pathway of recombinant human macrophage - colony stimulating - factor beta ( rhM - CSF beta ) by bis - cysteinyl modification and mass spectrometry . - pubmed
Melarsen oxide [ p -( 4,6 - diamino-1,3,5 - triazin-2 - yl ) aminophenylarsonous acid ( MEL ) ] , which selectively bridges spatially neighboring bis - cysteinyl residues in ( reduced ) proteins , was used to trap folding intermediates chemically during 1 ) time - dependent renaturation of recombinant human macrophage colony - stimulating factor ( rhM - CSF ) ; by redox refolding in vitro ; 2 ) reductive unfolding in the presence of the trapping reagent ; and 3 ) denaturing unfolding reactions in urea and guanidinium hydrochloride . - pubmed
In all folding and unfolding reactions a characteristic dimeric intermediate with two attached melarsen oxide ( MEL ) groups was observed , suggesting that these rhM - CSF beta species were important refolding intermediates . - pubmed
Cysteine residues C7 and C90 were minor modification sites . - pubmed
The mass spectrometric results from the in vitro folding reactions of rhM - CSF beta are in agreement with intrinsic tryptophan fluorescence measurements and are consistent with the folding pathway that starts with a fully reduced monomer ( R ) , includes partially folded monomeric intermediates ( M ) and dimeric intermediates ( D ) , and yields a final product with the native tertiary structure ( N ): 2R = = & gt ; 2 M = = & gt ; D = = & gt ; N. Our results show that selective chemical trapping of bis - thiol groups of proteins with MEL permits study of folding pathways by mass spectrometric structure characterization of intermediates with otherwise transient conformations . - pubmed
The topoisomerase 1 inhibitor , topotecan , has shown activity in an initial study . - pubmed
This study was therefore designed to determine whether the specific thromboxane A2 ( TP ) receptor antagonist , GR32191B , is effective in inhibition of prostanoid or nonprostanoid receptors in the RA . - pubmed
Two effects of GR32191B were tested : ( 1 ) the relaxation induced by GR32191B in the RA precontracted with the TP receptor agonists U46619 and PGF2alpha or nonprostanoid vasoconstrictors ( noradrenaline [ NA ] , angiotensin II [ AII ] , and K + ) and ( 2 ) the inhibitory effect of GR32191B on TP receptor agonists and nonprostanoid vasoconstrictors . - pubmed
RESULTS : In U46619 ( 10 nm , n=7 ) and PGF2alpha ( 1 microm , n=7 ) precontracted RA , GR32191B induced 100 % relaxation ( 10 - 100 microm ) but not after precontraction with nonprostanoid stimuli ( 5.8 % for K+ , 25 mm , n=6 , 24.4 % for NA , 3 microm , n=8 , and 53.2 % for AII , 3 nm , n=5 ) ( P<0.001 ) . - pubmed
However , GR32191B at 3 microm did not significantly inhibit the contraction induced by either NA , AII , or K+ . - pubmed
Biphenotypic B / macrophage cells express COX-1 and up - regulate COX-2 expression and prostaglandin E ( 2 ) production in response to pro - inflammatory signals . - pubmed
A potential role for B / macrophage cells in inflammation was shown by their ability to express prostaglandin H synthase-1 ( COX-1 ) and prostaglandin H synthase-2 ( COX-2 ) and by their production of prostaglandin ( PG ) E ( 2 ) . - pubmed
In contrast , COX-2 and the prostanoids PGE ( 2 ) , PGF ( 2alpha ) and PGD ( 2 ) are highly inducible in B/ macrophage cells upon stimulation with lipopolysaccharide , CD40 ligand , or via engagement of surface IgM , supporting a role for these cells in inflammation . - pubmed
PGD ( 2 ) and its metabolites are of interest because they activate the nuclear receptor PPARgamma that regulates lipid metabolism . - pubmed
Since PGE ( 2 ) blunts IL-12 production , its synthesis by B / macrophage cells may shift the balance of an immune response towards Th2 and humoral immunity . - pubmed
Electron microscopic evaluation of layers Ia and Ib revealed that mGluR4a was localized in synaptic terminals in layers Ia and Ib . - pubmed
Mutation of Tyr(191 ) of MMP-1 to Thr , the corresponding residue in MMP-3 , reduced collagenolytic activity about 5 - fold . - pubmed
Low density lipoprotein ( LDL ) particles are the major cholesterol carriers in circulation and their physiological function is to carry cholesterol to the cells . - pubmed
A new molecular interaction model for the beta - sheet structure and phosphatidylcholine headgroups is introduced and an overall view of the tertiary structure of apolipoprotein B-100 in the LDL particles is presented . - pubmed
Results revealed that 8.1 AH is associated with a high in vivo and in vitro production of TNF - alpha , which in turn seems responsible for increased serum levels of sELAM-1 , cortisol , and IL-10 . - pubmed
Thus , serum values seem to be the result of the effects of increased serum levels of TNF - alpha and cortisol . - pubmed
Signal transduction by the BvgAS system is characterized by a complex His - Asp - His - Asp phosphorelay . - pubmed
Ether extracts of lactic acid bacteria were analyzed for prevention of the oxidation of erythrocyte membrane and human low - density lipoprotein in vivo . - pubmed
A calcium sensor in the sodium channel modulates cardiac excitability . - pubmed
Calcium ions ( Ca2 + ) have a fundamental role in the coupling of cardiac myocyte excitation and contraction , yet mechanisms whereby intracellular Ca2 + may directly modulate Na channel function have yet to be identified . - pubmed
Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2 +/CaM - dependent slow inactivation , whereas the gating effects of Ca2 +/CaM were restored by intracellular application of a peptide modelled after the IQ domain . - pubmed
A naturally occurring mutation ( A1924 T ) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome , but at the same time inhibited slow inactivation induced by Ca2 +/CaM , yielding a clinically benign ( arrhythmia free ) phenotype . - pubmed
The beta - lactam resistance was due to production of beta - lactamases and ceftazidime proved to be stronger beta - lactamase inductor than ceftriaxone . - pubmed
This case suggests that interferon - alpha plus cytarabine ocfosfate therapy may be of benefit in the treatment of extramedullary blast crisis of CML . - pubmed
ACTH stimulation tests were carried out consecutively by using 250 microg and 1 microg intravenous ACTH as a bolus injection after an overnight fast , and blood samples were drawn at 0,30 and 60 min . - pubmed
The MN - rate was determined using the cytochalasin B ( cyt B ) block method . - pubmed
The validity of the test methods was ascertained by positive controls : mitomycin C ( MMC ) and bleomycin ( BLM ) were used in experiments without exogenous activation and cyclophosphamide ( CP ) in experiments with exogenous activation . - pubmed
OBJECTIVE : To examine the effects of graded doses of hydrocortisone ( HC ) on leptin secretion , and determine the effect of fasting . - pubmed
Blood sampling was performed every 1 to 2 hours for measurement of leptin , cortisol , insulin , and glucose levels . - pubmed
Separation of capsular polysaccharide K4 and defructosylated K4 derived disaccharides by high - performance capillary electrophoresis and high - performance liquid chromatography . - pubmed
Following chondroitinase digestion of K4 and its derivative , the two disaccharides , DeltaHexAFrc - GalNAc for K4 and deltaHexA - GalNAc for defructosylated K4 , are separated and readily determined within 20 min on an uncoated fused - silica capillary using normal polarity at 20 kV and detection at 230 nm . - pubmed
The described methods were used to evaluate the defructosylation process of K4 under drastic acid conditions . - pubmed
Determination of dissociation constants for a heparin - binding domain of amyloid precursor protein and heparins or heparan sulfate by affinity capillary electrophoresis . - pubmed
The 3 cholesterol stones ( case I ) and the 2 brown pigment stones ( case II ) showed macroscopic homogeneity , respectively . - pubmed
The 6 cholesterol stones ( case IV ) were divided into two types in size . - pubmed
One such mutant , hos2 , was shown previously to have an enhanced induction of stress - responsive genes by cold , whereas the expression of these genes under osmotic stress or the phytohormone absciscic acid ( ABA ) treatments was not affected . - pubmed
FIERY1 is a general negative regulator that controls cold , osmotic stress , and ABA signal transduction and possesses inositol polyphosphate 1 - phosphatase activity . - pubmed
Interestingly , the hos2 mutant protein is extremely tolerant to Li + . - pubmed
Serotonergic mechanism of the lateral parabrachial nucleus and relaxin - induced sodium intake . - pubmed
In the present study , we investigated the effects of bilateral injections of methysergide ( serotonergic receptor antagonist ) into the LPBN on intracerebroventricular ( i.c.v . ) - pubmed
Intracerebroventricular injections of relaxin ( 500 ng/1 microl ) induced water intake ( 5.1+/-0.7 ml/120 min ) , but not significant 1.8 % NaCl intake ( 0.5+/-0.4 ml/120 min ) . - pubmed
Bilateral injections of methysergide ( 4 microg/0.2 microl ) into the LPBN strongly stimulated relaxin - induced 1.8 % NaCl intake ( 34.5+/-10.9 ml/120 min ) and slightly increased water intake ( 10.5+/-4.9 ml/120 min ) . - pubmed
relaxin combined with LPBN methysergide on 1.8 % NaCl intake ( 0.5+/-0.4 ml/120 min ) . - pubmed
Losartan ( 50 microg/1 microl ) partially reduced the effects of relaxin . - pubmed
The results show that central relaxin interacting with central angiotensinergic mechanisms induces NaCl intake after the blockade of LPBN serotonergic mechanisms . - pubmed
New evidence that both T - type calcium channels and GABAA channels are responsible for the potent peripheral analgesic effects of 5alpha - reduced neuroactive steroids . - pubmed
Neurosteroids are potent blockers of neuronal low - voltage activated ( T - type ) Ca ( 2 + ) channels and potentiators of GABA ( A ) ligand - gated channels , but their effects in peripheral pain pathways have not been studied previously . - pubmed
Analgesic effects of ALPX were incompletely antagonized by co - injections of the GABA ( A ) receptor antagonist bicuculline . - pubmed
However , ( 3beta,5alpha,17beta )- 17 - hydroxyestrane-3 - carbonitrile ( ECN ) ) , which has effects on T - type channels but not on GABA ( A ) receptors , also induced potent enantioselective peripheral anti - nociception . - pubmed
Guanylyl cyclase C and accumulation of cGMP induced by bacterial heat - stable enterotoxins ( STs ) promote colon cancer cell cytostasis , serving as a tumor suppressor in intestine . - pubmed
Conversely , capacitative calcium entry through store - operated calcium channels ( SOCs ) is a key signaling mechanism that promotes colon cancer cell proliferation . - pubmed
Elimination of capacitative calcium entry employing 2 - aminoethoxydiphenylborate ( 2 - APB ) , a selective inhibitor of SOCs , potentiated cytostasis induced by ST . - pubmed
Opposition of ST - induced cytostasis by capacitative calcium entry reflects reciprocal inhibition of guanylyl cyclase C signaling . - pubmed
Calcium entry through SOCs induced by the calcium - ATPase inhibitor thapsigargin or the receptor agonists UTP or carbachol inhibited guanylyl cyclase C - dependent cGMP accumulation . - pubmed
Moreover , regulation by capacitative calcium entry reflected ligand - dependent sensitization of guanylyl cyclase C to inhibition by that cation . - pubmed
Although basal catalytic activity was refractory , ST - stimulated guanylyl cyclase C was inhibited by calcium , which antagonized binding of magnesium to allosteric sites required for receptor - effector coupling . - pubmed
These observations demonstrate that reciprocal regulation of guanylyl cyclase C signaling by capacitative calcium entry through SOCs represents one limb of a coordinated mechanism balancing colon cancer cell proliferation and cytostasis . - pubmed
They suggest that combining guanylyl cyclase C agonists and SOC inhibitors offers a novel paradigm for cGMP - directed therapy and prevention for colorectal tumors . - pubmed
CONCLUSIONS : This article represents the first prospective , multi - institutional study of HRQOL in men treated with PB and demonstrates that patients undergoing PB have a very high overall HRQOL . - pubmed
PAR produced a time- and dose - dependent increase of BDNF expression in the three hippocampal subfields examined . - pubmed
On the contrary , the effect of DMI on BDNF mRNA was neither dose- nor time - dependent . - pubmed
The results suggest that paroxetine , but not desipramine , enhances synaptic plasticity in the hippocampus by increasing BDNF mRNA expression , which determines a later AMPAR subunit trafficking to synaptic membranes . - pubmed
Others report that carbonic anhydrase II ( CA II ) binds to the C termini of the anion exchanger AE1 and the electrogenic Na / HCO3 cotransporter NBCe1 - A , enhancing transport . - pubmed
After injecting oocytes with NBCe1 - A cRNA ( Day 0 ) , we measured NBC current ( I(NBC ) ) by two - electrode voltage clamp ( Day 3 ) , injected CA II protein + Tris or just Tris ( Day 3 ) , measured I(NBC ) or the initial rate at which the intracellular pH fell ( dpH(i)/dt ) upon applying 5 % CO2 ( Day 4 ) , exposed oocytes to the permeant CA inhibitor ethoxzolamide ( EZA ) , and measured I(NBC ) or dpH(i)/dt ( Day 4 ) . - pubmed
Because dpH(i)/dt was greater in CA II than Tris oocytes , and EZA eliminated the difference , injected CA II was functional . - pubmed
Moreover , EZA had identical effects in CA II versus Tris oocytes . - pubmed
dpH(i)/dt was greater in oocytes expressing EGFP - e1 - CA II versus EGFP - e1 , and EZA eliminated the difference . - pubmed
Slope conductances of EGFP - e1 - CAII versus EGFP - e1 oocytes were indistinguishable , and EZA had no effect . - pubmed
Developmental rates of the IVF - derived ova to blastocysts and the survival rates of the frozen - thawed blastocysts were not different between the SOF medium ( 16 and 49 % ) and the co - culture system ( 13 and 61 % , respectively ) . - pubmed
To determine whether ADCON - L Gel ( ALG ) or Preclude Spinal Membrane ( PSM ) was more effective in preventing scarring , reducing pain , and improving quality of life postoperatively . - pubmed
Previous laboratory and clinical studies have found ALG and PSM to be effective , but none compared the two materials . - pubmed
PSM and ALG are equally effective in preventing epidural scarring associated with postdiscectomy syndrome . - pubmed
When the studies were combined according to similar measurement units , the overall mean decrease in malondialdehyde ( MDA ) level was -0.3 mM ( 95 % CI , -0.5 to -0.1 mM ; seven studies ) and -0.8 nmol / mg low - density lipoprotein ( LDL ) ( 95 % CI , -1.3 to -0.4 nmol / mg LDL ; three studies ) , respectively . - pubmed
A meta - analysis on the effect of Excebrane on pre - dialysis levels of oxidized - LDL could not be performed due to study heterogeneity . - pubmed
The selective cannabinoid CB ( 1 ) receptor antagonist , rimonabant , has undergone phase III clinical testing as anti - obesity drug . - pubmed
In this scenario , rimonabant 's anti - obesity action is accompanied by favorable changes in markers for insulin resistance , C - reactive protein , adiponectin , tumor necrosis factor alpha ( TNFalpha ) . - pubmed
A humanised monoclonal antibody to the HER2 receptor , trastuzumab , has demonstrable single - agent activity in metastatic breast cancer and enhances the antitumour effects of chemotherapy . - pubmed
The enzyme belongs to the " transglutaminase superfamily " that contains a putative catalytic triad of cysteine , histidine , and aspartic acid . - pubmed
The C - terminus of PNGase was recently found to be a novel carbohydrate - binding domain . - pubmed
The compound URB754 was recently identified as a potent inhibitor of the endocannabinoid - deactivating enzyme monoacylglycerol lipase ( MGL ) by screening of a commercial chemical library . - pubmed
Based on HPLC / MS , NMR and EI / MS analyses , the present paper shows that the MGL - inhibitory activity attributed to URB754 is in fact due to a chemical impurity present in the commercial sample , identified as bis ( methylthio ) mercurane . - pubmed
Retinoids exert their effects , in part , by binding to nuclear receptors , thereby altering gene expression . - pubmed
Manganese superoxide dismutase mRNA , protein , and activity levels increased in a time - dependent manner upon treatment with ATRA . - pubmed
Nuclear levels of the NF - kappaB proteins p50 and p65 increased within 24 h of ATRA administration . - pubmed
Furthermore an increase in DNA binding to a NF - kappaB element occurred within a 342 - bp enhancer ( I2E ) of the SOD2 gene with 10 microM ATRA treatment . - pubmed
Reporter analysis showed that ATRA - mediated I2E - dependent luciferase expression was attenuated upon mutation of the NF - kappaB element , suggesting a contribution of this transcription factor to retinoid - mediated upregulation of MnSOD . - pubmed
These results suggest that signaling events involving NF - kappaB and SOD2 may contribute to the effects of retinoids used in cancer therapy . - pubmed
Loss of ZFPL1 function also impairs cis - Golgi assembly following brefeldin A washout and slows the rate of cargo trafficking into the Golgi apparatus . - pubmed
Epo also enhances nitric oxide ( NO ) bioavailability through endothelial NO synthase transcription and activation , and exerts antiapoptotic actions through Bcl-2 and Bcl - XL . - pubmed
NO is a powerful vasodilator , insulin - sensitizer , inhibitor of atherothrombosis and apoptosis , and essential for progenitor mobilization . - pubmed
Furthermore , inhibition of JNK with a pharmacologic inhibitor or by selective knockdown blunted the efficacy of CGC-11093 and bortezomib . - pubmed
Therefore , CGC-11093 enhances the anticancer activity of bortezomib by augmenting JNK - mediated apoptosis and blocking angiogenesis . - pubmed
Glucagon - like peptide-1 ( GLP-1 ) , a 30 - amino - acid peptide regulates glucose homeostasis via control of insulin and glucagon secretion and by inhibition of gastric emptying and food intake . - pubmed
Oxyntomodulin ( OXM ) a 37 - amino - acid peptide also derived from the proglucagon gene , binds to both the glucagon and GLP-1 receptor ( GLP-1R ) ; however , a separate OXM receptor has not yet been identified . - pubmed
Here we show that OXM , like other GLP-1R agonists , stimulates cAMP formation and lowers blood glucose after both oral and ip glucose administration , actions that require a functional GLP-1R. - pubmed
Unexpectedly , OXM , but not the GLP-1R agonist exendin-4 , increased plasma levels of insulin after oral glucose administration . - pubmed
Biochemical studies show that this molecule inhibits NAD (+)- dependent DNA ligases , but not ATP - dependent enzymes . - pubmed
The molecule inhibits NAD (+)- dependent DNA ligases competitively with respect to NAD(+ ) and specifically inhibits enzyme adenylation , but not DNA adenylation or ligation . - pubmed
Labeling studies establish that this molecule inhibits the incorporation of thymidine into DNA and that overexpression of DNA ligase in the cell abolishes this inhibition . - pubmed
By terminal uridine deoxynucleotidyl transferase dUTP nick end labeling and flow cytometric analysis , higher apoptotic activity and a remarkable increase in sub - G(1 ) cell population were observed in transfected cells , suggesting that FSTL1 induced apoptosis in cancer cells . - pubmed
Moreover , in - cell NMR spectroscopy demonstrates that ubiquitin has much higher hydrogen exchange rates in the intracellular environment , possibly due to multiple interactions with endogenous proteins . - pubmed
In general , COOH - SWNT adsorb to the greatest extent , followed by OH - SWNT and P2 - SWNT . - pubmed
Colibactin acts as a cyclomodulin and blocks the eukaryotic cell cycle . - pubmed
Seven conformers of L - threonine in the gas phase : a LA - MB - FTMW study . - pubmed
Rotational spectroscopy in combination with molecular beams and laser ablation ( laser - ablation molecular - beam Fourier transform microwave ( LA - MB - FTMW ) spectroscopy ) has proved to be successful in characterizing the conformers of natural amino acids . - pubmed
erythrocyte Cu , Zn - Superoxide dismutase ( SOD ) , Glutathione peroxidase ( GSH - Px ) and catalase . - pubmed
In this study , we sought to clarify the nature of this abnormality by combining proton magnetic resonance spectroscopy ( 1H - MRS ) with functional magnetic resonance imaging ( fMRI ) at 3T. - pubmed
VO(2)AT , VO ( 2 )Peak , Load AT , Load peak and VE / VCO(2 ) slope were measured and compared . - pubmed
( 2 ) None of VO(2)AT , VO ( 2 )Peak , Load AT , Load peak and VE / VCO(2 ) slope is correlated with LVEF ; VO(2)AT , VO(2)Peak , Load AT and Load peak all correlate negatively with the higher NYHA grade ; VE / VCO(2 ) slope correlates positively with a higher NYHA grade ; None of VO(2)AT , VO ( 2 )Peak , Load AT , Load peak or VE / VCO(2 ) slope correlates with LVMI . - pubmed
Reactions of laser - ablated U atoms with P ( 4 ) molecules upon codeposition in excess argon gave weak new infrared absorptions at 504 , 483 , and 426 cm(-1 ) , which are best identified as binary uranium phosphide UP ( 4 ) species based on extensive B3LYP , BPW91 , and PBE density functional and CASSCF / CASPT2 wave function based calculations . - pubmed
Synthesis of core 2 branched O - glycans decorated by sLe ( X ) is dependent on C2GnT1 function and thus we determined enzyme activity of C2GnT1 . - pubmed
Gene knock - down of C2GnT1 in colon and hepatic carcinoma cells using short hairpin RNAs ( shRNA ) resulted in a 40 - 90 % decrease in C2 - O - sLe ( X ) and a 30 - 50 % decrease in E - selectin binding compared to control cells . - pubmed
Two - dimensional differential in - gel ( DIGE ) analysis identified 10 differentially expressed proteins between CHOP - sensitive and -resistant DLBCL cells that play roles in glycolysis ( triosephosphate isomerase-1 , enolase-1 ) , cytoskeletal structure ( ezrin , vimentin , tubulin - specific chaperone B ) , purine biosynthesis ( serine hydroxymethyltransferase ) , calcium binding ( sorcin ) , and apoptosis ( p53 , 14 - 3 - 3zeta , Akt ) . - pubmed
Knockdown of vimentin by siRNA or withaferin A repressed the invasiveness of CHOP - resistant cells in collagen matrices . - pubmed
More than 100 mg Nkg(dry soil(-1 ) ) of NH4 + addition resulted in the reduction of type II / type I MOB ratios and an obvious evolution of type II MOB communities , while less than 100 mg Nkg(dry soil(-1 ) ) of NH4 + addition induced nearly no change of methanotrophic community compositions . - pubmed
RESULTS : With IC-1.23 and IC-1.1 , the highest rates of aerosol production were achieved using FC75 ( 2.27 + -0.18 and 0.76 + -0.01 , respectively ) followed by PFOB ( 1.74 + -0.06 and 0.56 + -0.04 ) , PFD ( 0.82 + -0.01 and 0.21 + -0.01 ) , and surfactant ( 0.42 + -0.05 and 0.092 + -0.01 ) . - pubmed
Changes in PIP and RR directly influence the percentage of surfactant and perfluorocarbon recovered . - pubmed
We found that VVC- and MVC - selected amino acid changes map to different V3 locations and involve residues that interact with the CCR5 NT in different ways . - pubmed
These effects of DnaD were independent of the ability of DnaA to bind or hydrolyze ATP . - pubmed
We suggest that organisms in which DnaA has a high rate of nucleotide exchange predominantly regulate the DNA binding activity of DnaA and that those with low rates of exchange regulate hydrolysis and exchange . - pubmed
This report was the first to depict a PET / CT scan of a liver IMT that revealed an inhomogeneous , intense ( fluorine 18 )- fluoro-2 - deoxy - D - glucose uptake . - pubmed
gamma - Hydroxybutyric acid induces actions via the GABAB receptor in arousal and motor control - related nuclei : implications for therapeutic actions in behavioral state disorders . - pubmed
In addition , we recorded GHB - induced membrane responses using whole cell , patch clamp electrophysiology of immunohistochemically - identified principal neurons within these nuclei . - pubmed
GHB induced GABAB receptor - mediated rises in calcium in neurons of the LDT and the DR . - pubmed
GHB induced GABAB receptor antagonist - sensitive outward currents / hyperpolarizations in immunohistochemically - identified cholinergic LDT and serotonergic DR neurons . - pubmed
Finally , NCS-382 and HOCPCA , a reported antagonist and agonist specific to activity at the putative GHB receptor , respectively , with no demonstrated binding at the GABAB receptor , failed to block GHB - induced effects or elicit any discernible electrophysiological action when applied alone , indicating a lack of involvement of a GHB receptor in mediating GHB actions . - pubmed
total carbon ( TC ) & gt;13 g kg(-1 ) sediment , NH4 (+)-N & gt;144 mg kg(-1 ) sediment ) , while Group 1.1a- and Group 1.1a - associated sequences were dominant in sediments with opposite conditions ( e.g. - pubmed
TC & lt;4 g kg(-1 ) sediment , NH4 (+)-N & lt;93 mg kg(-1 ) sediment ) . - pubmed
The discovery that Rett syndrome ( RTT ) is caused by mutation of the methyl - CpG - binding - protein MeCP2 provided a major breakthrough in understanding the neurodevelopmental disorder and accelerated MeCP2 research . - pubmed
PI3K / AKT / mTOR signal pathway is an important intracellular signal transduction pathway with a significant role in cell proliferation , growth , survival , vesicle trafficking , glucose transport , and cytoskeletal organization . - pubmed
Specifically , we show that the 40Love / Samba carboxy - terminal domain ( GRD ) enables nucleocytoplasmic shuttling behavior . - pubmed
The ability of the protein to stimulate oxygen evolution in inside - out thylakoids was preserved throughout the various fractionation steps . - pubmed
We report here that U - II produces an increase in cytosolic Ca ( 2 + ) concentration in retrogradely labeled cardiac vagal neurons of nucleus ambiguus via two pathways : ( i ) Ca ( 2 + ) release from the endoplasmic reticulum via inositol 1,4,5 - trisphosphate receptor ; and ( ii ) Ca ( 2 + ) influx through P / Q - type Ca(2 + ) channels . - pubmed
For hybrid strain zygotes ( B6CBF1 ) communal culture in KSOM media supplemented with amino acids , albumin and Paf under 5 % O2 resulted in complete rescue of their rate of accumulation of cells and blastocyst formation . - pubmed
Apoptosis was detected by flow cytometry using annexin V and propidium iodide . - pubmed
RESULTS : Manumycin treatment resulted in significant decreases in the viabilities of the two prostate cancer cell lines in a dose - dependent manner through apoptosis , and this apoptosis involved caspase-9 activation . - pubmed
A specific inhibitor of caspase-9 protected cells from caspase-3 activation , apoptosis , and cytotoxicity induced by manumycin . - pubmed
We also found that manumycin downregulated Bcl-2 expression and upregulated Bax expression . - pubmed
CONCLUSION : Our data suggest that manumycin induces apoptosis in prostate cancer cells through regulation of the Bcl-2 family involving caspase-9 activation . - pubmed
A cation - anion configuration , whereby BF4 (- ) interacts with the C2 mpy cation from the side of the ring , is the most likely structure in the low - temperature phase IV region . - pubmed
In summary , this study shows that codon optimization strongly increases hNIS protein levels and radioiodine uptake , thus supporting opt - hNIS as a more sensitive reporter and efficient therapeutic gene . - pubmed
Moreover , the presence of polyanionic cellulose ( PAC ) further improved the rheological and filtration performances of CNC / BT - WDFs , suggesting a synergistic effect between PAC and CNCs . - pubmed
Studies examining the role of aspirin and HMG - CoA reductase inhibitors ( statins ) have shown important clinical differences in men versus women in the preventive realm . - pubmed
Histamine activates p38 MAP kinase and alters local lamellipodia dynamics , reducing endothelial barrier integrity and eliciting central movement of actin fibers . - pubmed
We tested the hypothesis that the barrier - disrupting agent histamine would reduce local lamellipodia protrusions and investigated the potential involvement of p38 mitogen - activated protein ( MAP ) kinase activation and actin stress fiber formation . - pubmed
The p38 MAP kinase inhibitor SB203580 attenuated the histamine - induced decreases in barrier function and lamellipodia protrusion frequency . - pubmed
SB203580 also inhibited the histamine - induced decreases in withdrawal distance and velocity , and the subsequent actin fiber migration . - pubmed
These data suggest that histamine can reduce local lamellipodia protrusion activity through activation of p38 MAP kinase . - pubmed
Likewise , in the TNFalpha - induced insulin - resistant 3T3 - L1 adipocyte model , BAPN prevented the downregulation of adiponectin and GLUT4 and the increase in SOCS3 levels , and consequently normalised insulin - stimulated glucose uptake . - pubmed
We compared steroid dependency ( relapse & lt;3 months of tapering first course CS or inability to wean & lt;10 mg prednisolone ) , need for IFX , linear growth , and surgical resections over the first 2 years . - pubmed
Synthesis of new 4 - phenylpyrimidine-2 ( 1H )- thiones and their potency to inhibit COX-1 and COX-2 . - pubmed
The objective of the present work was to evaluate physical properties , transport and in vitro modulator effects on NF - kappaB of poly ( lactide - co - glycolide ) ( PLGA ) nanoparticles loaded with lupeol . - pubmed
C4'-oxidized ( C4 - AP ) and C5'-oxidized abasic sites ( DOB ) that are produced following abstraction of a hydrogen atom from the DNA backbone reversibly form cross - links selectively with dA opposite a 3'-adjacent nucleotide , despite the comparable proximity of an opposing dA. - pubmed
A previous report on UvrABC incision of DNA substrates containing stabilized analogues of the ICLs derived from C4 - AP and DOB also indicated that the latter is repaired more readily by nucleotide excision repair [ Ghosh , S. , and Greenberg , M. M. ( 2014 ) Biochemistry 53 , 5958 - 5965 ] . - pubmed
In addition , the preferred DOB analogue is more reactive than the respective C4 - AP ICL , which is consistent with its more efficient incision by UvrABC . - pubmed
To further corroborate an extrapineal secretion of melatonin , the presence of the two key enzymes involved in melatonin synthesis , arylalkylamine - N - acetyltransferase ( AANAT ) and N - acetylserotonin - O - methyltransferase ( ASMT ) was analyzed by RT - PCR , q - PCR and Western - blot in ram testes , epididymis , and accessory glands . - pubmed
miR-155 Drives Metabolic Reprogramming of ER+ Breast Cancer Cells Following Long - Term Estrogen Deprivation and Predicts Clinical Response to Aromatase Inhibitors . - pubmed
Targeting the glycolysis - priming enzyme hexokinase-2 ( HK2 ) in combination with the AI , letrozole , synergistically reduced cell viability in AI - sensitive models . - pubmed
Both CSPs encoded an N - terminal signal peptide , four conserved cysteines , an OS - D superfamily domain , a 2Fe-2S ferredoxin domain , an anaphylatoxin domain and an EGF - like domain . - pubmed
Part 3 : Notch - sparing gamma - secretase inhibitors : SAR studies of 2 - substituted aminopyridopyrimidinones . - pubmed
In search for novel lead compounds as gamma - secretase inhibitors , analogs of aminopyrido [ 2,3 - d ] pyrimidin-7 - ones ( I ) were synthesized and evaluated for inhibitory effects on amyloid - beta - peptide production and cleavage of the Notch1 receptor mediated by gamma - secretase . - pubmed
Selected pyridopyrimidines , such as 1 , 8 , 9 , 10 , 11 and 16 are gamma - secretase inhibitors that did not have an effect on Notch1 receptor processing . - pubmed
Adiponectin Attenuates Angiotensin II - Induced Vascular Smooth Muscle Cell Remodeling through Nitric Oxide and the RhoA / ROCK Pathway . - pubmed
We investigated whether nitric oxide ( NO ) , the RhoA / ROCK pathway , actin cytoskeleton remodeling , and reactive oxygen species ( ROS ) mediate the anti - hypertrophic effect of APN . - pubmed
Ang II - induced protein synthesis was attenuated by pre - treatment with APN , NO donor S - nitroso - N - acetylpenicillamine ( SNAP ) , or cGMP . - pubmed
The hypertrophic response to Ang II was associated with a significant increase in RhoA activation and vascular force production , which were prevented by APN and SNAP . - pubmed
NO was also associated with inhibition of Ang II - induced phosphorylation of cofilin . - pubmed
Ang II - induced ROS formation and upregulation of p22 ( phox ) mRNA expression were inhibited by APN and NO . - pubmed
CONCLUSION : Our results suggest that the anti - hypertrophic effects of APN are due , in part , to NO - dependent inhibition of the RhoA / ROCK pathway and ROS formation . - pubmed
We also observed weak emission from the Er ( iii ) cation in the UV region for the first time in a Ln - MOF based material . - pubmed
Though this chapter covers a range of different peptides and protein , if focuses specifically on the opportunities afforded by the de novo design of coiled coils , miniature protein scaffolds , and the development on lanthanide - binding sites into these architectures . - pubmed
DISCUSSION : Recurrent elevations in cortisol in response to hypoglycemia may be the underlying pathology leading to suppression of gonadotropin - releasing hormone ( GnRH ) release . - pubmed
Oleanolic acid ( OA ) regulates inflammation and cellular dedifferentiation of chondrocytes via MAPK signaling pathways . - pubmed
Inhibition of p38 with SB203580 in OA - treated cells rescued the expression of type II collagen and suppressed the expression of COX-2 . - pubmed
However , ERK inhibition with PD98059 accelerated the OA - induced inflammatory responses . - pubmed
Film characterization with , among others , Fourier transform infrared ( FT - IR ) spectroscopy , X - ray diffraction ( XRD ) , and atomic force microscopy ( AFM ) attests to successful grafting of the polymers to the laponite nanoparticles . - pubmed
In particular , evidence of basal plane expansion of laponite with increasing silane concentration are obtained using XRD , while patent morphological changes are revealed with AFM . - pubmed
While LP - PEG - silane is easily applied to a surface from a precursor solution via a dip coating procedure LP - APTMS - OEGMA requires lots more chemicals , a thorough control of reaction parameters , and longer reaction time in order to generate films with the desirable properties . - pubmed
to surfaces is reduced by approximately 85 % in the case of LP - PEG - silane and up to 92 % in the case of LP - APTMS - POEGMA , in comparison to the control surfaces . - pubmed
We developed a paradigm that allows us to investigate the neuropharmacology by which previously neutral stimuli acquire the capacity to function as CSs and elicit ( intravenous ) cocaine conditioned approach and used this paradigm to test the role of NMDA receptor stimulation in the acquisition of cocaine conditioned approach . - pubmed
Blockade of NMDA receptors significantly impaired the acquisition of cocaine - conditioned approach as indicated by the emission of significantly fewer nose pokes and significantly longer latencies to nose poke during CS presentations . - pubmed
These results suggest that NMDA receptor stimulation plays an important role in the acquisition of reward - related conditioned responses driven by intravenous cocaine - associated CSs . - pubmed
When polyoxin AL , a chitin - synthesis - inhibitor , was added to the diet containing MLX56 family proteins , the toxicity of MLX56 family proteins disappeared and PM became thinner and fragmented . - pubmed
ALA - mediated biphasic downregulation of alpha-7nAchR / HIF-1alpha along with mitochondrial stress modulation strategy in mammary gland chemoprevention . - pubmed
The efficacy of ALA was scrutinized in vivo and in vitro using various experiments like hemodynamic studies , morphological analysis , antioxidants parameters , immunoblotting , and quantitative reverse transcription polymerase chain reaction . - pubmed
ALA treatment has also increased the level of synaptic acetylcholine and acetylcholine esterase with a significant decrease in lipid content . - pubmed
It was concluded that ALA persuaded the mitochondrial stress , activation of downstream cholinergic anti - inflammatory markers , and favorable regulation of hypoxia microenvironment through inhibition of fatty acid synthase and sterol regulatory element - binding protein . - pubmed
Postoperative factors of prognostic importance included hormone - related symptoms , stage IV disease , raised levels of 5 - HIAA , insufficiency of the tricuspid valve , secondary surgery not being macroscopically radical and a second malignancy . - pubmed
We performed fluorescent microscopy , Xpert MTB / RIF ( Xpert ) , Lowenstein - Jensen culture , human immunodeficiency virus , and random blood sugar testing on recruited patients . - pubmed
It was found that lactic acid ( Lac ) pretreatment combined with choline chloride / Lac system could not only selectively convert the hemicellulose to xylo - oligosaccharides ( XOS ) , which account for 89.7 % of total xylose in prehydrolysate ( the functional XOS ( DP & lt ; 5 ) took up about 35 % ) , but also significantly promote the glucose release ( 33.2 g/100 g material ) and well lignin separation ( representing 40.9 % in whole process ) , which better than the single organic pretreatment at higher modified severity index ( SI ) . - pubmed
One new 5 / 6 bicyclic sesquiterpene and its esterified derivative were characterised by GC - MS and 1D and 2D NMR spectroscopy . - pubmed
Raw PS flours were characterized by greater tannin and kafirin contents when compared to WS , and , after cooking , PS flours had greater resistant starch ( from 4.2 to 21.4 g /100 g dry matter ) , and lower starch hydrolysis index ( HI ) with respect to cooked WS . - pubmed
In addition , kafirin and total tannins content ( on raw ingredients ) along with the anthocyanin profiles ( on cooked samples ) were negative correlated with HI . - pubmed
Regulation of the SIRT1 signaling pathway in NMDA - induced Excitotoxicity . - pubmed
Silent Information Regulator 1 ( SIRT1 ) , an NAD+-dependent deacetylase , contributes to the neuroprotective effect . - pubmed
It is well known that N - methyl - D - aspartate ( NMDA ) receptor activation induces calcium influx which then activates PKC , and SIRT1 is a mRNA target for HuR protein . - pubmed
We hypothesize that Ca2 + -PKC - HuR - SIRT1 pathway modulates SIRT1 function . - pubmed
The results showed that : ( 1 ) SIRT1 levels were downregulated in NMDA model ; ( 2 ) NMDA induced an increase in serine phosphorylation of HuR , while inhibition of serine phosphorylation of HuR increased SIRT1 levels , promoting cell survival ; ( 3 ) PKC inhibitor ( Go 6976 ) reversed NMDA insults and also suppressed serine phosphorylation of HuR ; ( 4 ) 1,2 - bis ( 2 - aminophenoxy ) ethane - N , N , N ' , N '- tetraacetic acid ( BAPTA - AM ) , an intracellular calcium chelator , fully reversed NMDA insults and also inhibited PKC activity evoked by NMDA . - pubmed
These results indicate that intracellular elevated Ca2 + activates PKC , which phosphorylates HuR and then promotes SIRT1 mRNA decay and subsequent neuronal death in NMDA model . - pubmed
Therefore , the study suggests that inhibition of Ca2 + -PKC - HuR - SIRT1 pathway could be an effective strategy for preventing certain neurological diseases related to NMDA excitotoxicity . - pubmed
The results showed that , in contrast to the control group ( SC ) , the BITC - treated group ( SQ_BITC ) had 197 differentially expressed genes ( DEGs ) , of which 115 were downregulated and 82 were upregulated . - pubmed
TRIM21 and PHLDA3 negatively regulate the crosstalk between the PI3K / AKT pathway and PPP metabolism . - pubmed
Here , we demonstrate the important reciprocal crosstalk between the PI3K / AKT signal and pentose phosphate pathway ( PPP ) branching metabolic pathways . - pubmed
PPP metabolites , in turn , reinforce AKT activation and further promote cancer metabolic reprogramming by blocking the expression of the AKT inhibitor PHLDA3 . - pubmed
Twenty - four patients were treated with cetuximab with 8/167 ( 4.7 % ) identified as being treated with a RAS variant ( n = 3 ) or incomplete testing ( n = 5 ) ; thus , not in accordance with guidelines . - pubmed
Serological tests for serum creatinine , ALT , and AST were performed . - pubmed
Thrombosis in post - transcatheter aortic valve replacement ( TAVR ) patients has been correlated with flow stasis in the neo - sinus . - pubmed
The results showed that all post - TAVR anatomical measurements were significantly different in patients with and without thrombus , but only sinus diameter had a linear correlation with thrombus volume ( r = 0.471 , p = 0.008 ) . - pubmed
Thus , root anatomy , reactive oxygen species detection by fluorescence microscopy and , ROS histochemical staining along with the NADPH oxidase activity were analyzed . - pubmed
BACKGROUND : PRAME ( PReferentially expressed Antigen in MElanoma ) has shown utility in distinguishing melanoma from benign melanocytic lesions , but knowledge of its expression pattern in intermediate melanocytic and spitzoid proliferations is limited . - pubmed
This study explores the potential to reach a circular economy for post - consumer Recycled Polyethylene Terephthalate ( rPET ) packaging and bottles by using it as a Distributed Recycling for Additive Manufacturing ( DRAM ) feedstock . - pubmed
It was found that AGM and its enzyme - degraded products , though with different molecular weights , had similar beta - glycosidic bonds and monosaccharide compositions , exerted similar strength of hypoglycemic effects , and reinstated with a similar extent the disordered gut microbiota and the contents of SCFAs and endogenous metabolites . - pubmed
Overall , our observation indicated that CYP or MAT could disturb the hormonal balance , and their combination of 0.1 mug / L CYP +25 mug / L MAT could significantly enhance the estrogenic effect . - pubmed
By hemagglutination , hemagglutination inhibition , and immunofluorescence techniques , it was shown that the anti - VH antiserum detected three sets of antigens in the VH region , namely idiotypic antigens , VH subgroup - specific antigens , and VH domain-(framework ) specific antigens . - pubmed
The release of TG showed significant correlations with the uptake of glycerol . - pubmed
The second extraction step with 4M - guanidinium chloride yielded only bands I and II . - pubmed
It is found by means of different methods ( the administration of 35S - heparin , the determination of fibrinolytic activity of blood plasma fractions containing fibrinogen degradation products and fibrinogen -- heparin complex , spectral analysis of fibrinogen -- heparin complex etc . ) - pubmed
Results obtained all the conclusion that two homeostatic equilibrium of L - carnitine family components , namely the inter - exchange between L - carnitine and its esters catalyzed by carnitine acyl transferases , and a saturable tubular reabsorption process with differentiated threshold for each component . - pubmed
omega - CT had no effect on either the electrically paced contractile response that was elicited by direct muscle stimulation or the enhancement of the paced response caused by beta - adrenoceptor agonist stimulation . - pubmed
Neomycin also caused insurmountable inhibition of responses elicited by beta - adrenoceptor agonist stimulation . - pubmed
In the vas deferens , ileum and atria the inhibitory effects of omega - CT were long - lasting , whereas the effects of neomycin could be reversed upon wash - out . - pubmed
Although the PHA - induced mitogen response of lymphocytes from the other three lymphoid compartments remained suppressed throughout the study period , the response to the mitogens Con - A and PWM generally returned to normal or supranormal levels by the seventh postburn day , Therefore it appears that the effect of a thermal injury on lymphocyte function varies according to the lymphocyte compartment examined and the mitogen tested . - pubmed
Even at an etodolac concentration of 80 micrograms / ml , DNA synthesis , proteoglycan synthesis , and type - II collagen synthesis were unchanged . - pubmed
Collagenase production was also unaffected by etodolac ( 60 micrograms / ml ) . - pubmed
The principle of the colorimetric method depends on the determination rate of p - nitrophenol released on hydrolysis of N - CBZ - L - leucine - p - nitrophenyl ester at pH 8.0 by subtilisin , with correction for any nonenzymatic ( spontaneous ) hydrolysis of the substrate . - pubmed
Thymidine kinases purified from the different sources were similarly sensitive to inhibition by dTTP or 5 '- AdThd and to deinhibition of the dTTP - induced regulation of enzyme activity by 5 '- AdThd . - pubmed
In none of these cultures was nucleoside phosphorylase activity detected , as indicated by the inability of the cells to produce thymine or iodouracil after exposure to the appropriate nucleosides . - pubmed
Also , 5 '- AdThd did not affect the breakdown of dTMP by crude preparations of cytosolic 5 '- nucleotidase from the different cells . - pubmed
We found that intracellular dTTP pools in the various cell types were substantially high ( 15 - 26 microM ) compared to the sensitivity of thymidine kinase to inhibition by dTTP ( IC50 2 - 4 microM ) . - pubmed
To test the sensitivity of thymidine kinase ( in situ ) to regulation by dTTP we investigated : ( a ) the effect of depleting intracellular dTTP pools with methotrexate on the uptake of thymidine ( dThd ) ; and ( b ) the effect of pH on the uptake of dThd and its perturbation by 5 '- AdThd , since the inhibition of thymidine kinase activity by dTTP is known to be pH dependent . - pubmed
Sodium dodecylsulphate ( SDS ) , a component in IL-2 excipient , was found to cause a short term breach in the BBB , enabling the penetration of viruses into the brain . - pubmed
Predicting bone marrow iron stores in anemic patients in a community hospital using ferritin and erythrocyte sedimentation rate . - pubmed
Forty - three consecutive cases from a community hospital with concomitant bone marrow iron stain , serum ferritin , and erythrocyte sedimentation rate ( ESR ) were reviewed . - pubmed
A two - dimensional linear graphic relationship between ferritin and ESR correctly identified six of nine iron - deficient patients and 32 of 34 iron - present patients . - pubmed
Lupus anticoagulants represent a subclass of anti - phospholipid antibodies which are characterized by their ability to prolong the clotting time in in vitro coagulation assays measuring partial thromboplastin time ( PTT ) ( Thiagarajan , P. , Shapiro , S. S. , and DeMarco , L. ( 1980 ) J. Clin . - pubmed
Competition experiments , using a modified PTT assay , demonstrated that hexagonal phase phospholipids , including natural and synthetic forms of phosphatidylethanolamine , were able to neutralize the lupus anticoagulant activity of all 11 hybridoma antibodies . - pubmed
Thus , these antibodies are capable of recognizing phospholipids on purely structural criteria . - pubmed
The demonstration that anti - phospholipid antibodies are able to distinguish between different structural arrangements of phospholipid may have important implications regarding the immunoregulation of autoimmunity . - pubmed
Met - enkephalin - like immunoreactivity in neurointermediate pituitary is decreased by DA receptor stimulation . - pubmed
Bromocriptine treatment resulted in a dramatic decline of Met - enkephalin - LI in neurointermediate pituitary which was significant by 3 days of treatment . - pubmed
The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity . - pubmed
Glucose administration ( 3 g / kg , by gavage , a dose sufficient to cause insulin - mediated reductions in plasma levels of the large neutral amino acids leucine , isoleucine , and valine ) also elevated brain phenylalanine and tyrosine , and enhanced the increments caused by the aspartame , nearly doubling the rise in brain phenylalanine . - pubmed
Norepinephrine and renin activity in chronic renal failure . - pubmed
The significance of PRA was further assessed by angiotensin blockade with saralasin . - pubmed
Lectin binding strain - specific carbohydrates on the cell surfaces of Leishmania strains from the Old World . - pubmed
The applied demetallization procedures ( dialysis against 0.1 M HCl or 1 M acetic acid and against 0.1 M EDTA following by dialysis against 1 M acetic acid ) had no effect on the stability of the lectin subunit structure , as proved by ultracentrifugation analysis or thin - layer chromatography on Sephadex G-200 Superfine . - pubmed
Affinity electrophoresis on polyacrylamide gel containing immobilized alpha - D - galactosyl residues and affinity chromatography on alpha - D - galactosyl derivative of Separon have shown that the interaction of the demetallized lectin preparations does not differ from that of the native lectin in alkaline media but is decreased in acidic media . - pubmed
CKA encodes a protein that is 80 % identical at the amino acid level to the human Mel1a melatonin receptor and is thus designated the chick Mel1a melatonin receptor . - pubmed
The widespread distribution of CKA and CKB mRNA in brain provides a molecular substrate for the profound actions of melatonin in birds . - pubmed
An association between alcohol and high - density lipoprotein cholesterol was found only in the latest survey and probably reflects the aging of the cohort . - pubmed
Analysis with multiple linear regression showed that all variables with the exception of triglycerides , HDL cholesterol and height were significantly associated with systolic blood pressure . - pubmed
[ Cys5 , Cys11 ] Dyn A1 - 11 - NH2 ( 1 ) and [ Cys5 , D - Ala8 , Cys11 ] Dyn A1 - 11 - NH2 ( 2 ) are not only highly potent kappa - selective peptides but they also exhibit exceptional selectivity for kappa receptors in the central ( brain ) vs. the peripheral ( ileum ) systems . - pubmed
The chromosome aberration assay ( CA ) in bone marrow cells and the micronucleus test ( MNT ) in both bone marrow and peripheral blood erythrocytes have been carried out for the evaluation of the clastogenic potential of acephate ( asataf ) in a chick in vivo test system . - pubmed
Co - infusion with BQ-123 ( 0.1 mg kg-1 min-1 ) prevented the rise in MAP produced by big ET-1 and completely blocked the renal response . - pubmed
Similarly , BQ-123 inhibited both the increase in MAP and the decrease in RPF and GFR produced by ET-1 . - pubmed
Big ET-1 but not ET-1 , produced a significant increase in water and sodium excretion . - pubmed
BQ-123 had no effect on the diuretic and natriuretic response to big ET-1 consistent with possible ETB - mediated inhibition of tubular reabsorption . - pubmed
We have previously shown that at higher doses of ET-1 , BQ-123 was unable to inhibit the renal vasoconstrictor response despite blockade of the pressor response . - pubmed
We conclude that serotoninergic neurotransmission plays an important part in post - stroke pathological crying and that citalopram is an effective and well - tolerated treatment . - pubmed
When both extra- and intracellular Ca2 + were depleted , the activation of p72syk was still persistent ; in contrast , the deactivation process was completely abrogated even at 6 min after stimulation . - pubmed
In addition , genistein and herbimycin A , potent protein - tyrosine - kinase inhibitors , were capable of reducing concanavalin A - evoked p72syk activation and Ca2 + mobilization as well as the aggregation and lysozyme release . - pubmed
These lines of evidence suggest that p72syk is activated with concanavalin A in a Ca ( 2 +)-independent manner , participating in a mechanism of Ca2 + recruitment , and negatively regulated by a feedback mechanism through Ca2 + in neutrophils . - pubmed
Vicinal dihaloalkanes are transformed by glutathione S - transferase - catalyzed reactions to mutagenic and nephrotoxic S -( 2 - haloethyl ) glutathione S - conjugates . - pubmed
The first step is hepatic glutathione S - conjugate formation followed by cleavage to the corresponding cysteine S - conjugates , and , after translocation to the kidney , metabolism by renal cysteine conjugate beta - lyase . - pubmed
Beta - Lyase - dependent metabolism of halovinyl cysteine S - conjugates yields electrophilic thioketenes , whose covalent binding to cellular macromolecules is responsible for the observed toxicity of the parent compounds . - pubmed
Finally , hepatic glutathione conjugate formation with hydroquinones and aminophenols yields conjugates that are directed to gamma - glutamyltransferase - rich tissues , such as the kidney , where they undergo alkylation or redox cycling reactions , or both , that cause organ - selective damage . - pubmed
Blood glucose and serum insulin levels were identical in both sessions . - pubmed
The rate of conversion of [ 14C ] arachidonic acid to [ 14C ] PGE2 ( PGE2 synthase ) was determined in cell sonicates under optimal in vitro conditions . - pubmed
After a treatment period of 4 h we observed an increase in PGE2 production in the presence of mEGF ( 4.7 - fold ) , VAN ( 1.8 - fold ) or both agents ( 6.8 - fold ) over control samples . - pubmed
Similarly , the specific activity of PGE2 synthase was stimulated maximally after 4 h with mEGF ( 2.1 - fold ) , VAN ( 1.7 - fold ) or with both ( 4.8 - fold ) compared with untreated tissue samples . - pubmed
We suggest , that VAN and mEGF act by a mechanism(s ) that involves de novo protein synthesis or that alters the phosphorylation state of enzymes that are requisite for the conversion of arachidonic acid to PGE2 . - pubmed
BP was measured with a mercury sphygmomanometer after a 10 min rest three times in 5 min . - pubmed
decreased levels of parathyroid hormone , calcitonin and the transport form of vitamin D3 . - pubmed
Parathion ( PA ) is a phosphorotioate pesticide requiring P-450 - mediated oxidations to become activated to paraoxon , or to be metabolised to its less toxic metabolites . - pubmed
On the other hand , sodium arsenite [ As ( III ) ] markedly decreases total hepatic P-450 content and dependent monoxygenase activities . - pubmed
Our aim was to determine the effects of As ( III ) pretreatment on the acute toxicity of PA and its possible relationship with the effects of As ( III ) on P-450 - dependent monooxygenase activities . - pubmed
As ( III ) pretreatment increased the acute toxicity of PA , reducing 38 % its median lethal dose ( LD50 ) from 11.68 to 7.21 mg PA / kg . - pubmed
In addition , As ( III ) pretreatment further decreased the inhibitory effect of PA on brain acetylcholinesterase activity , reducing 33 % the median inhibitory dose ( ID50 ) from 3.44 to 2.31 mg PA / kg . - pubmed
As ( III ) decreased the P-450 content to 87 % of control values , reduced EROD activity to 74 % and BROD activity to 41 % ; PA produced no significant effects on these parameters , whereas the joint administration of As ( III )+ PA produced effects similar to those of As ( III ) . - pubmed
PROD activity was reduced to 36 % of control value by PA , whereas As ( III ) alone produced no significant effects . - pubmed
Stimulation of lipolysis in vitro by the effectors isoproterenol with or without adenosine deaminase , dibutyryl cAMP with or without isobutylmethylxanthine , and forskolin was not altered by ST treatment . - pubmed
The computational time was short ( approximately 60 s for the prediction of proteins with 200 amino acid residues ) . - pubmed
Growth hormone reserve was assessed by at least two stimulation tests ( clonidine , L - dopa , growth hormone - releasing hormone ) . - pubmed
The GH response to clonidine was significantly less than that found with the other stimuli . - pubmed
The growth rate in conjunction with the GH response to clonidine provides a sensitive measure of GHD associated with chemotherapy . - pubmed
We conclude that C / EBPalpha is required for the cAMP induction of PEPCK gene expression in the liver and that C / EBPbeta can compensate for the loss of C / EBPalpha if its concentration is induced to appropriate levels . - pubmed
Plasma glucose concentrations decreased after 100 and 150 micrograms kg-1 IGF-1 from 3.40 to 2.50 and 2.34 mmol l-1 , respectively ( SED 0.314 , P & lt ; 0.001 ) , but were not significantly altered after lower doses . - pubmed
The degree of early - phase bronchial responsiveness to allergen ( the cumulated dose of allergen causing a 20 % fall in FEV1 [ PD20allergen ] ) was related to the degree of nonspecific bronchial responsiveness ( the concentration of histamine causing a 20 % fall in FEV1 [ PC20histamine ] ) and the level of specific IgE or allergen thresholds as found in skin tests and basophil histamine release assays . - pubmed
Weak correlations were found between PD20allergen and the level of specific IgE ( r = -0.36 , p = 0.07 ) or allergen thresholds as found in skin tests ( skin prick test , r = 0.36 and p = 0.07 ; intracutaneous test , r = 0.49 and p = 0.01 ) or basophil histamine release assays ( r = 0.37 , p = 0.08 ) . - pubmed
Moreover , no significant contribution of these indices of IgE - mediated hypersensitivity to the prediction of PD20allergen by multilinear regression models with PC20histamine was found . - pubmed
Nitric oxide ( NO ) is a multifunctional molecule that is synthesized by a family of enzymes , namely nitric oxide synthases ( NOS ) . - pubmed
NO modulates the expression of bcl-2 family members , p53 , interleukin-1 beta - converting enzyme family proteases and the cytokine receptor Fas . - pubmed
Therefore , NO generated from NO donors or synthesized by NOS induces cell death via apoptosis in a variety of different cell types . - pubmed
The electronic absorption spectra of these hemoproteins were similar to those of native MnP , but absorption maxima were shifted to longer wavelengths in the order of Deut - rMnP , MPP - rMnP , Etio - rMnP , DME - rMnP , and rMnP. - pubmed
All enzymes contained a high - spin , pentacoordinate heme iron as evidenced by the characteristic charge transfer bands in the visible region . - pubmed
The hemoproteins exhibited reduced catalytic activity while maintaining similar substrate Km values compared to native MnP. - pubmed
Compounds I , II , and III were obtained for these hemin - analogue enzymes except for Deut - rMnP. - pubmed
Histamine plays an important role in bronchoconstriction mediated by histamine receptors which provoke bronchial asthma attack . - pubmed
Glucose - induced thermal stabilization of the native conformation of GLUT 1 . - pubmed
It is concluded that the shift to a higher denaturation transition temperature in the presence of D - glucose is not a result of increased kinetic stability of GLUT 1 . - pubmed
In addition , the redox regulation of inactivation , which is typical for Kv1.4 channels , can be conferred to the inactivation of the deleted constructs by incorporation of an N - terminal cysteine residue . - pubmed
Barrier disruption effected by dioctanoyl ( di - C8 ) phosphatidate was markedly potentiated by the addition of propranolol , an inhibitor of endothelial cell " ecto "- phosphatidate phosphohydrolase ( PAP ) , a lipid phosphate phosphohydrolase ( LPP ) that efficiently hydrolyzes extracellular substrate . - pubmed
Phosphatidate - induced permeability was attenuated by preincubation of endothelial cells with the tyrosine kinase inhibitor , herbimycin A ( 10 microg / ml ) , and enhanced by the tyrosine phosphatase inhibitor , vanadate ( 100 microM ) , implicating a role for activation of intracellular tyrosine kinases in the response . - pubmed
In addition , phosphatidate increased the levels of intracellular free Ca ( 2 + ) in endothelial cells and ligated specific binding sites on endothelial cell plasma membranes , consistent with the presence of a phosphatidate receptor . - pubmed
We conclude that phosphatidate may be a physiologic modulator of endothelial monolayer permeability that exerts its effects by activating a receptor - linked , tyrosine kinase - dependent process which results in mobilization of intracellular stored Ca ( 2 +)and consequent metabolic activation . - pubmed
GC and GC / MS analysis indicated that the cuticular hydrocarbons with chain lengths & gt;21 carbons were the same as those found in the hemolymph . - pubmed
Glucose oxidation ( micromol / h per mg protein ; mean ( SEM ) ) on day 7 of DSS administration was significantly higher than in controls ( 0.06 ( 0.006 ) v 0.027 ( 0.004 ) , p<0.001 ) . - pubmed
Production of beta - hydroxybutyrate was decreased and production of lactate increased in DSS colitis compared with controls . - pubmed
Increasing butyrate concentration from 10 to 80 mM enhanced oxidation in DSS colitis ( 0.036 ( 0.002 ) to 0.285 ( 0.040 ) , p<0.001 ) , although it continued to remain lower than in controls . - pubmed
When 1 mM DSS was added to normal colonocytes in vitro , it did not alter butyrate oxidation . - pubmed
Abnormal butyrate oxidation became apparent only after six days of DSS administration , at which time histological abnormalities were more widespread . - pubmed
MRS was employed to measure the level of real - time intestinal ATP and pH in vivo . - pubmed
In group C , tissue pH was significantly lower than in other groups during I / R. CONCLUSION : Leukocytosis induced by G - CSF exerts a prolonged effect on ATP during I / R and leukocyte depletion helps protect against the I / R injury . - pubmed
Did nucleotides or amino acids drive evolutionary conservation of the WT1 + /-KTS alternative splice ? - pubmed
One highly conserved feature of the Wilms ' tumour suppressor gene , WT1 , is the potential , following alternative pre - mRNA splicing , to insert three amino acids ( KTS ) between the third and fourth zinc fingers . - pubmed
No sera showed antibodies that reacted to only PAH . - pubmed
An immunocompetition assay demonstrated that the reactivity against PAH represents a cross - reactivity with TPH , whereas antibodies against TPH and TH are directed towards epitopes unique for the two enzymes . - pubmed
Two series of fungal metabolites , enniatins and beauvericins , were found to be ubiquitous and potent inhibitors of ABC transporters . - pubmed
To study the role of these interactions in DNA transfer and to identify essential amino acids of VirB8 , we introduced random mutations in virB8 by the mutagenic PCR method . - pubmed
Analysis of the mutant strains by DNA sequence analysis , Western blot assays , and reconstruction of new point mutations led to the identification of five amino acid residues that are essential for VirB8 function . - pubmed
The substitution of glycine-78 to serine , serine-87 to leucine , alanine-100 to valine , arginine-107 to proline or alanine , and threonine-192 to methionine led to the loss of VirB8 activity . - pubmed
TASK-5 , a new member of the tandem - pore K (+ ) channel family . - pubmed
Four TASKs ( TASK1 - 4 ) identified so far form functional K (+ ) channels and encode background K (+ ) channels in various cell types . - pubmed
TASK-5 shares 51 % amino acid identity with TASK-1 and TASK-3 . - pubmed
Thus , TASK-5 is a new member of the tandem - pore K (+ ) channel family but does not produce a functional plasma membrane K (+ ) current by itself . - pubmed
The physical association between the two enzymes alters the DNA / topoisomerase II interaction as shown by pulse - field electrophoresis after incubation of intact nuclei with the specific topoisomerase II inhibitor VM-26 . - pubmed
We previously developed a formula to estimate the individual carboplatin clearance ( CL ) based on serum creatinine ( Scr ) determined by an enzymatic assay using creatinine amidohydrolase . - pubmed
After subretinal injection , both lenti - VSVG and AAV2/5 transduced the retinal pigment epithelium ( RPE ) and photoreceptors efficiently whereas transgene expression was restricted to RPE cells using lenti with the Mokola envelope or AAV2/1 . - pubmed
The icv infusion of the selective glucocorticoid receptor antagonist RU38486 blocked the hyperphagic effects of twice - daily icv injections of DEX in both sexes . - pubmed
Resistant isolates , containing the tcrB gene , grew on brain heart infusion agar plates containing up to 28 mM CuSO4 compared to only 4 mM for the susceptible isolates . - pubmed
The present study was designed to evaluate the link between platelet activation and GD3 expression and to verify whether this ganglioside might play a role in modulating signal transduction events . - pubmed
Our results suggest that following platelet activation , GD3 is rapidly expressed on the platelet surface and internalised to the cytoskeleton where it transiently associates first with the Src family tyrosine kinase Lyn then with the Fc receptor gamma chain . - pubmed
These data drive us to speculate that GD3 might act as second messenger in the activatory cascade , which leads to CD32 expression and triggers platelet adhesion and spreading to the subendothelial matrix . - pubmed
For dose - corrected concentrations ( C / D ) and for clearance ( Cl ) we found the coefficient of variation ( CV ) to be approximately 55 % for all variables ( C / D CIT , C / D DCIT , the ratio DCIT to CIT , and for Cl CIT ) . - pubmed
On a population level , signs of a possible saturation of CYP2D6 associated with increasing DCIT - to - CIT ratios with increasing daily doses was observed . - pubmed
Finally , concomitant medication affected the outcome of serum concentrations by a general increase in C / D CIT and C / D DCIT but without alteration in the DCIT - to - CIT ratio . - pubmed
BACKGROUND : The association between sympathetic activation and mortality in chronic heart failure and the favorable effect of beta blocking drugs has raised the possibility of therapeutic efficacy for central sympathetic inhibition with sustained - release ( SR ) moxonidine , an imidazoline receptor agonist . - pubmed
Final analysis revealed 54 deaths ( 5.5 % ) in the moxonidine SR group and 32 deaths ( 3.4 % ) in the placebo group during the active treatment phase . - pubmed
Hospitalization for heart failure , acute myocardial infarction and adverse events were also more frequent in the moxonidine SR group . - pubmed
Plasma norepinephrine was significantly decreased by moxonidine SR ( -18.8 % from baseline ) vs. placebo ( +6.9 % ) . - pubmed
Nonetheless , the excess early mortality and morbidity suggest the likelihood of an adverse effect of moxonidine SR and raise concerns regarding the efficacy of generalized sympathetic inhibition in heart failure . - pubmed
Two independent methods have been critically evaluated and applied to the measurement of total homocysteine in serum and plasma : solid - phase anion extraction ( SPAE ) gas chromatography / mass spectrometry ( GC / MS ) and protein precipitation liquid chromatography / tandem mass spectrometry ( LC / MS / MS ) . - pubmed
On the basis of the excellent precision and close agreement of the mass spectrometric ( MS ) methods , the MS - based methods will be used for certification of a serum - based SRM for homocysteine and folates . - pubmed
These data suggest that : ( i ) The actions of zotepine in discrimination assays are similar to , but not identical with , those of clozapine and quetiapine ; ( ii ) The differences among the actions of clozapine , quetiapine and zotepine may be related to either the unique ability of zotepine to block noradrenaline ( NA ) uptake , or to its more marked affinity for D ( 2 ) receptors ; ( iii ) The finding that zotepine only mimicked quetiapine up to a level of 54 % was unexpected , since quetiapine and clozapine generalise reciprocally and zotepine generalised fully to ( low dose ) clozapine . - pubmed
[ Therapeutic use of ACTH and cortisone ] . - pubmed
Paraoxon and phenylacetate were used as substrate for measuring PON1 activities and 1 - hexadecyl-2 -[ 3H ] acetyl - glycero-3 - phosphocholine for platelet - activating factor acetylhydrolase ( PAF - AH ) activity . - pubmed
PON1 activities correlated positively and significantly with HDL - cholesterol , HDL2 - cholesterol , HDL3 - cholesterol , HDL - phospholipids , apo A - I , apo A - II , and LpA - I : A - II . - pubmed
Enzymatic hydrolysis of gelatin layers on used lith film using thermostable alkaline protease for recovery of silver and PET film . - pubmed
To develop a new efficient and potential industrial enzymatic process for the recovery of silver and poly ( ethylene terephthalate ) ( PET ) from used lith film for printing , which has not been recycled at all , enzymatic hydrolysis of gelatin layers on lith film was investigated using the thermostabilized mutant enzyme of the alkaline protease from alkaliphilic Bacillus sp . - pubmed
Administration of NKT cell ligands , alpha - galactosylceramide ( alpha - GalCer ) resulted in the activation of both cytokine production and natural killing . - pubmed
Intracellular staining experiments demonstrated that NKT cells were the earliest source of both IL-4 and IFN - gamma production after alpha - GalCer administration in vivo . - pubmed
In contrast , isolated NK1.1+CD3- classical NK cells exhibited greatly enhanced natural killing activity 6 h after alpha - GalCer administration . - pubmed
NKT cells , however , exhibited a strong cytotoxicity when they were activated and expanded with alpha - GalCer plus IL-2 in vitro . - pubmed
These results indicated that NKT cells act as regulatory cells via production of cytokines for activation of NK cell - mediated cytotoxicity in vivo at early phase after alpha - GalCer administration . - pubmed
The combination of HSP70 overexpression in situ and tumor killing by thymidine kinase / ganciclovir treatment , but neither strategy alone , provoked potent systemic antitumor activities after intratumor injection of Ad - HT . - pubmed
The epitope of HMMC-1 was determined by transfection with a panel of glycosyltransferase cDNAs and by inhibition assays with chemically synthesized oligosaccharides . - pubmed
CONCLUSIONS : These results indicate that HMMC-1 specifically recognizes a novel O - glycan structure . - pubmed
Fluorescence and CD spectroscopic analysis of the alpha - chymotrypsin stabilization by the ionic liquid , 1 - ethyl-3 - methylimidazolium bis [( trifluoromethyl ) sulfonyl ] amide . - pubmed
The stability of alpha - chymotrypsin in the ionic liquid , 1 - ethyl-3 - methyl - imidizolium bis [( trifluoromethyl ) sulfonyl ] amide ( [ emim ][ NTf2 ] ) , was studied at 30 and 50 degrees C and compared with the stability in other liquid media , such as water , 3 M sorbitol , and 1 - propanol . - pubmed
The kinetic analysis of the enzyme stability pointed to the clear denaturative effect of 1 - propanol , while both 3 M sorbitol and [ emim ][ NTf2 ] displayed a strong stabilizing power . - pubmed
The [ emim ][ NTf2 ] enhanced both the melting temperature and heat capacity of the enzyme compared to the other media assayed . - pubmed
The fluorescence spectra clearly showed the ability of [ emim ][ NTf2 ] to compact the native structural conformation of alpha - chymotrypsin , preventing the usual thermal unfolding which occurs in other media . - pubmed
While the tyrosine phosphorylation of STAT3 , which is indicative of its signal - dependent activation , is a common occurrence in tumors , and appears to play a crucial role in some malignancies , a variety of new data suggest that it can be dispensable under some circumstances and STAT3 can participate in transformation through novel and non - canonical mechanisms . - pubmed
U0126 , a specific inhibitor of MEK1 / 2 , downregulated CAIX expression induced by true hypoxia and cell density . - pubmed
Mapping of the U0126 effect revealed that both critical elements within the CA9 promoter , the hypoxia response element and the juxtaposed SP1 - binding PR1 , were inhibited . - pubmed
Most importantly , release of pyridoxal-5 '- phosphate and protein thermal denaturation were found to occur simultaneously at pH 6.0 in contrast to pH 8.5 where denaturation preceded cofactor 's release by approximately 3 degrees C. To correlate the observed differences in thermal denaturation with structural features , the crystal structure of phosphoserine aminotransferase was determined at 1.2 and 1.5 A resolution at two different pHs ( 8.5 and 4.6 , respectively ) . - pubmed
Missense mutations in the Notch3 gene that create or destroy cysteine residues , have been found in most cases with a family history of the disease , although a few sporadic cases harbouring Notch3 mutations have also been described . - pubmed
SSE for SCs is similar to that described previously for PCs in that it is prevented by BAPTA and DAG lipase inhibitors in the recording pipette ; however , unlike in PCs , NMDA receptors ( NMDARs ) play an important role in SSE for SCs . - pubmed
Contrasting to normal CA , normal TA showed lower endothelial calcium signaling with more concentrated NF - kappaB in the endothelial nuclei . - pubmed
Molecular Dynamics simulations in aqueous solution were performed for the matrix metalloproteinase-8 ( MMP-8 ) free catalytic domain and for its complexes with the ( R )- and ( S )-[ 1 -( 4 '- methoxybiphenyl-4 - sulfonylamino )- 2 - methylpropyl ] phosphonate . - pubmed
Glucose regulation of CDK7 , a putative thiol related gene , in experimental diabetic nephropathy . - pubmed
MAIN OUTCOME MEASURES : Relationships between serum iron , adiposity , and serum transferrin receptor , C - reactive protein , ferritin , and iron intake analyzed by analysis of covariance and multiple linear regression . - pubmed
RESULTS : Serum iron was lower ( 75.8+/-35.2 vs 86.5+/-34.2 g / dl , P=0.002 ) , whereas transferrin receptor ( 22.6+/-7.1 vs 21.0+/-7.2 nmol / l , P=0.026 ) , C - reactive protein ( 0.75+/-0.67 vs 0.34+/-0.67 mg / dl , P<0.0001 ) and ferritin ( 81.1+/-88.8 vs 57.6+/-88.7 microg / l , P=0.009 ) were higher in obese than non - obese subjects . - pubmed
Obese subjects had a higher prevalence of iron deficiency defined by serum iron ( 24.3 % , confidence intervals ( CI ) 19.3 - 30.2 vs 15.7 % , CI 11.0 - 21.9 % , P=0.03 ) and transferrin receptor ( 26.9 % , CI 21.6 - 33.0 vs 15.7 % , CI 11.0 - 21.9 % , P=0.0078 ) but not by ferritin ( 9.8 % , CI 6.6 - 14.4 vs 9.3 % , CI 5.7 - 14.7 % , P=0.99 ) . - pubmed
Transferrin receptor , ferritin and C - reactive protein contributed independently as predictors of serum iron . - pubmed
B19 - infected plasma was used to test whether incubation of amotosalen with virus before PCT enhanced inactivation compared to immediate PCT . - pubmed
CONCLUSION : Under defined conditions , PCT with amotosalen combined with UVA light can be used to inactivate B19 , a clinically significant virus that can be transmitted through blood transfusion , and heretofore has been demonstrated to be refractory to inactivation . - pubmed
In the present study we aimed to assess the role of MMPs in experimental cardiomyopathy induced by repeated 10 - week administration of daunorubicin ( 3 mg / kg i.v . ) - pubmed
In the daunorubicin group , the plasma cardiac troponin T levels ( cTnT - a marker of myocardial necrosis ) were significantly increased ( p<0.05 ) , commencing with the 8th administration compared with the controls . - pubmed
The amount of collagen ( an estimate of fibrosis ) was also significantly higher in the daunorubicin group ( 13.39 + /- 0.97 mg / g wet weight ) compared to the control group ( 10.03 + /- 0.65 mg / g wet weight ) . - pubmed
Evaluation of the proposed method was performed by manual segmentation of chronic and enhanced ( early ) acute lesions in gadolinium enhanced T1 - weighted ( Gad - E - T1 - w ) images by studying T1 - weighted ( T1 - w ) and T2 - weighted ( T2 - w ) images , using similarity criteria . - pubmed
AIM : To determine whether endurance training can counterbalance the negative effects of hypoxia on mitochondrial phosphorylation and expression of the long chain mitochondrial fatty acid transporter muscle carnitine palmitoyl transferase 1 ( mCPT-1 ) . - pubmed
RESULTS : Chronic hypoxia decreased basal ( V(0 ) , -31 % with pamitoylCoA + carnitine and -21 % with palmitoylcarnitine , P & lt ; 0.05 ) and maximal ( V(max ) , -31 % with pamitoylCoA + carnitine , P & lt ; 0.05 ) respiration rates , hydroxyacylCoA dehydrogenase activity ( -48 % , P & lt ; 0.05 ) , mCPT-1 activity index ( -34 % , P & lt ; 0.05 ) and mCPT-1 protein content ( -34 % , P & lt ; 0.05 ) . - pubmed
BACKGROUND : Based on the outcome of several randomized controlled trials , the orally active leukotriene receptor antagonist montelukast ( Singulair , Merck ) has been licensed for treatment of asthma . - pubmed
Pharmacological differences of glitazones : does peroxisome proliferator - activated receptor - alpha activation make the difference ? - pubmed
RESULTS : Two - week preoperative treatment with tiotropium significantly improved respiratory symptoms and pulmonary function as reflected by FVC ( median 3.43 L pretreatment vs 3.52 L post - treatment ) , FEV(1 ) ( median 2.06 L vs 2.32 L ) and FEV(1)% ( 73.2 % vs 81.0 % ) ( all P & lt ; 0.001 ) . - pubmed
Enzyme activities of acetyl - CoA carboxylase ( ACC ) and fatty acid synthase ( FAS ) as well as diacylglycerol acyltransferase ( DGAT ) and 3 - hydroxy-3 - methyl - glutaryl - CoA reductase ( HMG - CoA - R ) , pace - setting steps of de novo fatty acid , triacylglycerol ( TAG ) and cholesterol synthesis respectively were assayed in digitonin - permeabilized hepatocytes . - pubmed
It has been demonstrated that ORP150 binds tumor - associated Ag peptides within cancer cells . - pubmed
Most recently , it has been shown that exogenous ORP150 induces cross - presentation of a chaperoned Ag , thereby stimulating Ag - specific CTLs . - pubmed
Our data indicate that targeting of the Ag to a " static " early endosme by ORP150 is required for the efficient cross - presentation . - pubmed
Nonlinear Hammett plots in pyridinolysis of 2,4 - dinitrophenyl X - substituted benzoates : change in RDS versus resonance contribution . - pubmed
The Bronsted - type plots for pyridinolysis of 1a - j are linear with beta(nuc ) = 0.74 - 0.98 , indicating that the reaction proceeds through a stepwise mechanism in which the second step is the RDS . - pubmed
It has been concluded that the electronic nature of the substituent X in the benzoyl moiety does not influence the RDS , but the degree of bond formation ( or the effective charge on the nucleophilic site ) in the transition state becomes more significant as the substituent X changes from a strong EDG to a strong EWG . - pubmed
Development of specific inhibitors of CYP707A , a key enzyme in the catabolism of abscisic acid . - pubmed
Catabolic inactivation of ABA is mainly controlled by ABA 8 '- hydroxylase ( CYP707A ) , which is the cytochrome P450 catalyzing the C8'-hydroxylation of ABA into 8 '- hydroxy - ABA and its more stable tautomer , phaseic acid , which has much lower hormonal activity than ABA . - pubmed
Thus , a specific inhibitor of CYP707A is promising not only as a chemical probe for the mechanism of ABA action , but also because of its potential use in agriculture and horticulture . - pubmed
The novel histone deacetylase inhibitor , AR-42 , inhibits gp130 / Stat3 pathway and induces apoptosis and cell cycle arrest in multiple myeloma cells . - pubmed
Preclinical data indicate cisplatin sensitivity , suggesting that these tumors may have defects in the BRCA1 pathway . - pubmed
Heme - regulated eukaryotic initiation factor 2alpha kinase ( HRI ) functions under conditions of heme shortage caused by blood diseases such as erythropoietic protoporphyria and beta - thalassemia , and retains the heme : globin ratio at 1:1 by sensing the heme concentration in reticulocytes . - pubmed
It was found that [ 1 ] an interaction between Hsp90 and phosphorylated HRI was evident , whereas no interaction was observed between Hsp90 and HRI dephosphorylated by treatment with lambda protein phosphatase ; [ 2 ] Hsp90 enhanced the kinase activity of phosphorylated HRI but not dephosphorylated HRI , but this enhancement was not observed in the presence of heme ; and , [ 3 ] autophosphorylation of HRI was not influenced by Hsp90 . - pubmed
Therefore , we propose that autophosphorylation of HRI is critical for catalytic regulation by Hsp90 under heme - shortage conditions . - pubmed
We showed that Naph exhibited effective preferential cell growth inhibition via G2 / M phase arrest and apoptosis , which was associated with reduced levels of Cdc2 and Cdc25C expression . - pubmed
Naph also increased cleaved caspase-3 and Poly ADR ( adenosine diphosphate ribose ) Polymerase expression , gamma - H2AX expression ( an indicator of DNA double strand breaks ) and DNA fragmentation . - pubmed
The cell lines were transfected or irradiated with US with pGL3 control ( luciferase reporter plasmid ) , and a luciferase assay ( 48 h later ) or an MTS assay ( 72 h later ) were performed . - pubmed
Design and evaluation of a series of pyrazolopyrimidines as p70S6 K inhibitors . - pubmed
High throughput screening versus p70S6 K led to the identification of aminopyrimidine 3a as active inhibitor . - pubmed
We included 576 subjects ( 229 controls , 193 AD , and 154 mild cognitive impairment subjects who converted to AD ) from the ADNI study , for whom baseline data on cerebrospinal fluid amyloid - beta ( Abeta)42 , phosphorylated tau ( p - tau ) , and total - tau ( t - tau ) , hippocampal volumes , and FDG - PET were available . - pubmed
To ensure the quality of the filter system every 7 days metabolic parametres such as WBC count , haemoglobin content , haemolysis rate , potassium load , pH and ATP content were analysed over a storage period of 49 days . - pubmed
During whole storage time the ATP level was maintained above 2.0 mumol per g haemoglobin . - pubmed
RESULTS : Lysozyme was found to be substantially bound onto negatively charged vesicles via electrostatic interaction as evidenced by zeta potential measurements regardless of cholesterol content . - pubmed
In contrast , the size of phospholipid vesicle - bound lysozyme became larger with the increasing cholesterol content . - pubmed
Furthermore , serum high - sensitivity C - reactive protein , 8 - isoprostane and superoxide dismutase were similar among the three tertiles of patients ( all P & gt ; 0.05 ) . - pubmed
Enzymatic assays of RcLPAT2 indicate a preference for ricinoleoyl - CoA over other fatty acid thioesters when ricinoleoyl - LPA is used as the acyl acceptor , while oleoyl - CoA is the preferred substrate when oleoyl - LPA is employed . - pubmed
RcLPATB groups with B - class LPAT enzymes described as seed specific and selective for unusual fatty acids . - pubmed
However , RcLPATB exhibit a broad specificity on the acyl - CoAs , with saturated fatty acids ( 12:0 - 16:0 ) being the preferred substrates . - pubmed
RcLPATB is upregulated coinciding with seed triacylglycerol accumulation , but its expression is not restricted to the seed . - pubmed
abortus 2308 , MucR regulates a wide variety of genes whose products may function in establishing and maintaining cell envelope integrity , polysaccharide biosynthesis , iron homeostasis , genome plasticity , and transcriptional regulation . - pubmed
The hypothesis for the enhanced stereoselectivity has been that the benzylidene locks the molecule in a less reactive conformation with the O6 trans to the ring oxygen ( O5 ) , which would stabilize the formed alpha - triflate and subsequent give beta - selectivity . - pubmed
In this work , the hypothesis is challenged by using the carbon analogue ( C7 ) of the benzylidene - protected mannosyl donor , which is investigated in terms of diastereoselectivity and reactivity and by low - temperature NMR . - pubmed
Furthermore , the mdVp1s formed multiprotein complexes with LT , Hsp70 , and Hsp40 , and a fraction of the largest mdVp1 , md4 , was disulfide linked to the unmodified Vp1DeltaC. - pubmed
Only cotreatment , but not the single exposure to limonene and linalyl acetate , replicated distinctive morphological and biochemical changes induced by BEO , including caspase-3 activation , PARP cleavage , DNA fragmentation , cell shrinkage , cytoskeletal alterations , together with necrotic and apoptotic cell death . - pubmed
The data suggest that the endolymphatic enlargement caused by a loss of Slc26a4 is a consequence of disrupted Na (+ ) transport . - pubmed
The collateral perfusion pressure changes to AVP were lower in STZ - injected groups , which were reversed by OPC-31260 ( a V2R antagonist ) and overcome by NaF ( a G protein activator ) . - pubmed
3 and 4 are able to react with 9 - ethylguanine ( 9 - EtG ) yielding the corresponding monoadduct [ Pt ( dmba )( L )( 9 - EtG )](+ ) derivatives as followed by ESI - MS . - pubmed
Compound 3 interacts mainly with double - stranded ( DS ) oligonucleotides as shown by analysis with ESI - TOF - MS , being also able to displace ethidium bromide ( EB ) from DNA , as observed by an electrophoretic mobility study . - pubmed
Theoretical calculations at the COSMO(CHCl3)/B3LYP - D / def2 - TZVPPecp//B3LYP - D / def2 - TZVPecp level and energy evaluations at the COSMO(CHCl3)/PWPB95 - D3 / def2 - TZVPPecp level of theory on compound 4 and model systems have been done . - pubmed
This was mainly due to postnatal increases in LA - containing PC and PE , resulting in decreased fractions of their DHA- and ARA - containing counterparts . - pubmed
These changes are also reflected by PE molecular composition as an indicator of altered hepatic fatty acid homeostasis . - pubmed
A surface receptor crosslinking assay was conducted to examine the response of individual NMDA receptor subunits to dopamine depletion in isolated and confined surface and intracellular compartments of striatal neurons . - pubmed
The NMDA receptor GluN2B subunit was elevated in its abundance in the surface pool of the lesioned striatum , while intracellular GluN2B levels were not altered . - pubmed
CONCLUSION : These results demonstrate the differential sensitivity of principal NMDA receptor subunits to dopamine depletion . - pubmed
GluN1 and GluN2B expression in the distinct surface compartment underwent upregulation in striatal neurons after selective lesions of the dopaminergic pathway by 6 - OHDA . - pubmed
MATERIALS AND METHODS : In this experimental study , we have treated hESCs with 200 nM of Stauprimide ( Spd ) for one day followed by activin A ( 50 ng / ml ; A50 ) for the next three days ( Spd - A50 ) . - pubmed
The use of higher concentrations of Spd ( 400 and 800 nM ) in the Spd - A50 protocol caused an increase in the expression SOX17 as well as a dramatic increase in mortality rate of the hESCs . - pubmed
Therefore , while the Spd - A50 protocol led to DE production , we have shown that IDE1 / 2 could not fully replace activin A in DE induction of hESCs . - pubmed
Structure - activity relationship ( SAR ) analysis showed that the anti - proliferative activity was affected by A - ring - substituent ( -OCH3>-CH3>-H>-Br>-Cl>-F ) . - pubmed
Docking simulation of compound 7d into EGFR active site showed that naphthalene ring of 7d with LYS721 formed two p - pi bonds , which enhanced antitumor activity . - pubmed
Various thioglycoside derivatives were obtained in high yields by one - pot fluoride - mediated de - silylation and thiol alkylation with alkyl halides or Michael acceptors of one common TIPS S - glycoside . - pubmed
Additional serum and plasma samples were used to measure concentrations of thromboxane B2 ( TXB2 ) and prostaglandin E2 ( PGE2 ) , to assess COX-1 and - 2 inhibition , respectively . - pubmed
N -[ 3,4 - dimethoxycinnamoyl ]- anthranilic acid ( tranilast ) inhibits transforming growth factor beta , alleviates allergic reactions , and decreases hypertrophic skin scars . - pubmed
Histological evaluation of ionized calcium binding adaptor molecule 1 ( Iba1 ) at 1 week after SCI and glial fibrillary acidic protein ( GFAP ) , fibronectin , and chondroitin sulfate ( CS ) at week 8 was performed . - pubmed
At week 8 , although the number of GFAP - positive cells increased greatly from the impaction site to the proximal and distal sites in the control group , these cells were confined around a cavity in the tranilast - treated groups . - pubmed
Anti - CS antibody level in the tranilast - treated groups was significantly lower than that in the control group . - pubmed
Levels of serotonin ( 5HT ) , norepinephrine ( NE ) and dopamine ( DA ) were quantified in the CSF after 1 h of sham , standard and microburst VNS with a wash - out period of 1 month . - pubmed
One week after the CSF experiment , the PTZ seizure threshold was determined after the same stimulation paradigm . - pubmed
SIM ( selected ion monitoring ) was applied to isolate fatty acid methyl esters on TIC ( total ion current ) chromatograms , using the 74 Da fragment ion , which originated from McLafferty rearrangement , and is typical for methyl - esters . - pubmed
GC - MS system was used for the trans - esterification of triglycerides to fatty acid methyl esters in the gas chromatographic injector . - pubmed
STUDY DESIGN : Intact deciduous teeth with different root resorption stages were cryopreserved using FBS and Dimethyl Sulfoxide ( DMSO ) medium ( 9:1 ) in a progressive freezing process , by placing the samples in the refrigerator ( 4 degrees C/60 min ) and subsequently transferring them to a -80 degrees C freezer ( controlled device -1 degrees C / min/24 hours ) , and finally into liquid nitrogen ( -196 degrees C/30 days ) . - pubmed
Chromatographic separation of antimalarial drugs and internal standard ( ibuprofen ) was achieved on a C18 column with a mobile phase composed of 10 mM dipotassium orthophosphate at pH 3.0 , methanol , and acetonitrile in a ratio of 20:38:42 v / v , at a flow rate of 1 mL / min . - pubmed
The mechanism of action of DSF for treating these addictions is unclear but it is unlikely to involve the inhibition of liver aldehyde dehydrogenase ( ALDH2 ) . - pubmed
An internal standard , the N , N - di - isopropylcarbamoyl homolog ( MIM : 291>128 ) is easily separable from DETC - NAC ( MIM : 263>100 ) on C18 RP media with a methanol gradient . - pubmed
However , these techniques are known to be problematic when applied to highly polymorphic genomic regions , such as the human leukocyte antigen ( HLA ) genes . - pubmed
Reactions between 2,6 - diformyl-4 - alkyl ( R )- phenol ( R = CH3 or C ( CH3 ) 3 ) and 1,3 - diamino-2 - hydroxypropane ( 1,3 - DAP ) in the presence of copper ( II ) salts ( Cu ( BF4 ) 2 6H2O , Cu ( ClO4 ) 2 6H2O / H3BO3 / Ar ) and triethylamine ( TEA ) in a single pot result in self - assembly of dimeric dodecacopper supramolecular architectures of 30 - membered hexatopic macrocyclic ligands ( H6L4 and H6L5 ) with unique and fascinating structures having the BO3 ( 3 - ) anion as the central species bonded to all six copper centers in a symmetrical fashion ( mu6 - BO3 ( 3 - ) ) . - pubmed
Thereafter , the rewarding effects of nicotine were evaluated through the conditioned place preference ( CPP ) and self - administration ( SA ) tests . - pubmed
In stark contrast , MEL1 hES cell amino acid and carbohydrate use and mitochondrial function were relatively unaltered in response to oxygen . - pubmed
Here , we have demonstrated that Hra1 mediates autoaggregation by self - association and we hypothesize that at least some surface - exposed amino acid sequences that are present in Hra1 , but absent in Tia , represent autoaggregation motifs . - pubmed
In Hra1 we swapped nine candidate motifs in three of these loops , ranging from one to ten amino acids in length , to the corresponding sequences in Tia . - pubmed
METHOD : We primarily assessed whether pitavastatin could help mitigate the development of emphysema induced by continuous cigarette smoking ( CS ) exposure . - pubmed
CONCLUSION : Increased AQP1 and AQP4 levels in the heart tissue may be correlated with the maintenance of water and electrolytes balance , which decreases with aging . - pubmed
TRP channels are classified into seven subfamilies based on the amino acid sequence homology , and transient receptor potential melastatin 2(TRPM2 ) is the second member of the TRPM subfamily . - pubmed
The single target lysine of PanB was identified by a combination of mass spectroscopy and mutational analysis . - pubmed
Titanium dioxide nanoparticles ( TiO2 NP ) are broadly used in a wide range of applications . - pubmed
In this study we investigated cytotoxicity , genotoxicity and epigenetic properties of TiO2 NP uncoated and coated with silica or citrate , as well as of the benchmark material P25 . - pubmed
Our results showed that the uncoated TiO2 NP were both cytotoxic and genotoxic , and the remediation strategy adopted did not reduce the adverse effects of uncoated TiO2 NP . - pubmed
In particular , the presence of citrate was able to increase their cytotoxicity and genotoxicity , exerting also epigenotoxic effects , as evaluated by the marked reduction of LINE-1 methylation levels . - pubmed
The structural , optical , and excitonic characteristics of the resultant MAPbI3 thin films were analyzed using X - ray diffraction ( XRD ) , atomic - force microscopy , absorbance spectroscopy , photoluminescence ( PL ) spectrometry , and nanosecond time - resolved PL . - pubmed
AIM : To optimize the production of pH - sensitive dapsone ( DAP ) nanoparticles based on Eugradit L100 ( NPs - EL100 - DAP ) for oral delivery . - pubmed
RESULTS : The in vitro release assay of NPs - EL100 - DAP confirmed the nanoparticles ' pH sensitivity and the ability to deliver DAP at intestinal environment . - pubmed
OBJECTIVE : Tofacitinib is an oral JAK inhibitor indicated for the treatment of rheumatoid arthritis ( RA ) . - pubmed
The dimeric excerpt { ( mu - bipy )[ FeL1 ( MeOH )] 2 } 2MeOH of the coordination polymer chain crystallizes in the triclinic space group P1 and reveals that the packing of the molecules in the crystal is dominated by hydrogen bonds . - pubmed
Effects of glucose - insulin infusion during major oral and maxillofacial surgery on postoperative complications and outcomes . - pubmed
Our aim is to elucidate the effects of intraoperative glycemic control by glucose - insulin ( GI ) infusion on postoperative complications and outcomes in major oral and maxillofacial surgery . - pubmed
In the GI group , regular insulin was continuously applied with glucose - added acetate Ringer 's solution ( 5 - 10 g glucose per 500 mL ) . - pubmed
The mean infusion rate of glucose during surgery ( mg / kg / h ) was independently associated with the decrease in both serum total protein and albumin concentrations from the control to Day1 . - pubmed
Conclusions : Application of additional glucose during major oral and maxillofacial surgery preserved serum albumin concentration . - pubmed
Simply linking the two aromatic rings of a diarylamine to afford a phenoxazine offers profound differences in HAT reactivity : 1000 - times greater than diphenylamine and 10 - fold more reactive than alpha - tocopherol , Nature 's optimized phenolic RTA . - pubmed
Thus , phenoxazines are an exciting scaffold for RTA development . - pubmed
In recent years , we have found that many organosulfur compounds exhibit very good RTA activity . - pubmed
In particular , sulfenic acids ( RSOH ) and hydropersulfides ( RSSH ) are found to be among the best HAT agents known , particularly to peroxyl radicals where secondary orbital interactions are found to play a significant role . - pubmed
These insights suggest that the RTA activity of organosulfur compounds may be as important to the inhibition of hydrocarbon autoxidation , if not more so , than their ionic reactions . - pubmed
We identified 545 differentially expressed genes ( DEGs ) , 33 of which were in common between the nMnO2 - Cd , nHAP20 - Cd , and nHAP40 - Cd groups . - pubmed
Blockage of HGF signaling by HGF receptor inhibitor , PHA-665752 , inhibited proliferation of hESC - RPE cells . - pubmed
Doxorubicin ( DOX ) , a model anticancer drug , was first covalently linked onto the partially acetylated poly ( amidoamine ) dendrimers of generation 5 ( G5 ) prefunctionalized with folic acid ( FA ) through acid - sensitive cis - aconityl linkage to form G5 NHAc - FA - DOX conjugates , which were then entrapped with gold ( Au ) nanoparticles ( NPs ) to create dendrimer - entrapped Au NPs ( Au DENPs ) . - pubmed
Importantly , because of the coexistence of targeting ligand FA and Au core NPs as a CT imaging agent , the multifunctional DOX - loaded Au DENPs afford specific chemotherapy and CT imaging of FA receptor - overexpressing cancer cells . - pubmed
The constructed DOX - conjugated Au DENPs hold a promising potential to be utilized for simultaneous chemotherapy and CT imaging of various types of cancer cells . - pubmed
Parvalbumin and lipoprotein lipase could be firstly selected as key proteins in view of their known functions in regulating the buffering of intracellular free Ca2 + in both cartilage and bone cells and bone mass , respectively . - pubmed
Apoptosis in germ cells was quantified by the transferase biotin - dUTP nick end labeling assay . - pubmed
Lactate modulates CD4 + T - cell polarization and induces an immunosuppressive environment , which sustains prostate carcinoma progression via TLR8 / miR21 axis . - pubmed
Here we demonstrate that in a prostate cancer model lactate released by glycolytic cancer - associated fibroblasts ( CAFs ) acts on CD4 + T cells , shaping T - cell polarization . - pubmed
In particular , CAFs exposure ( i ) reduces the percentage of the antitumoral Th1 subset , inducing a lactate - dependent , SIRT1 - mediated deacetylation / degradation of T - bet transcription factor ; ( ii ) increases Treg cells , driving naive T cells polarization , through a lactate - based NF - kB activation and FoxP3 expression . - pubmed
This hypothesis was confirmed by the fact that furfural and malonate , inhibitors of pyruvate dehydrogenase and succinate dehydrogenase ( P cycle enzymes ) , respectively , increased resistance of VA - RCAZ to antibiotics , while exposure to triclosan , to inhibit biosynthesis of fatty acids , decreased resistance . - pubmed
Oxygen concentration ( 0 , 0.5 % and 1 % ) of ionized gas was firstly confirmed to be the main factors for CP treatment , besides treatment time ( 2 , 4 and 6 min ) . - pubmed
In terms of anthocyanin and vitamin C , relatively shorter exposure time to CP was more suggested . - pubmed
CONCLUSIONS : The PRISTINE study demonstrated the feasibility of adding rifampicin to beta - lactam antibiotics in the treatment of community - acquired pneumococcal pneumonia , but , despite solid in vitro and experimental animal research evidence , failed to demonstrate a difference in plasma LTA concentrations and subsequent inflammatory and clinical responses . - pubmed
In addition , LTA plasma concentration could be too low to mount a response via Toll - like receptor 2 in vitro , but may nonetheless have an effect in vivo . - pubmed
Our findings show that the relative expression of SGLT1 and GLUT2 mRNA were not affected by fructose - feeding and/or chrysin . - pubmed
This is the first report showing that chrysin is able to interfere with the effects of fructose at the intestinal level , which may contribute to the fructose - induced MS features . - pubmed
Silicone oil or rubber with PDMS can be combined with NR - latex and CR - latex because of PVA 's emulsion polymerization behavior . - pubmed
New pyrimidine - benzoxazole / benzimidazole hybrids : Synthesis , antioxidant , cytotoxic activity , in vitro cyclooxygenase and phospholipase A2 - V inhibition . - pubmed
Exaggerated activation of the renin angiotensin aldosterone system ( RAAS ) is a key feature in diseases such as hypertension , diabetes and chronic kidney disease . - pubmed
Mitochondrial oxygen consumption was evaluated after addition of Ang II alone or after pre - incubation with candesartan ( AT1 receptor antagonist ) , PD-123319 ( AT2 receptor antagonist ) or the two in combination . - pubmed
Ang II response was reversed to increased oxygen consumption by nitric oxide synthase inhibitor L - NAME . - pubmed
In addition , it promoted the differentiation and activation of CD8 + T lymphocytes , differentiation of CD14 + monocytes , and stimulated the release of nitric oxide and cytokines , mainly those linked to a Th1 response . - pubmed
AIM : This study aimed to evaluate the effect of lithium disilicate endocrowns compared to prefabricated zirconia crown used for restoring pulpotomized primary molars , on their Fracture Resistance and to compare the loads to failure these different ceramic restorations with previously reported posterior occlusal forces . - pubmed
Group ( G1 ) and the lithium disilicate ( IPS e.max Press ) Endocrown Group ( G2 ) . - pubmed
RESULTS : Group zirconia crown ( G1 ) showed significantly higher fracture strength than Group ( G2 ) lithium disilicate endocrown ( p < 0.05 ) . - pubmed
Ribociclib ( LEE011 , Kisqali ) is a highly selective small molecule inhibitor of cyclin - dependent kinases 4 and 6 ( CDK4 / 6 ) , which has been approved for the treatment of advanced or metastatic breast cancer . - pubmed
Circuits were primed with blood , albumin , heparin , NaHCO3 , and CaCl2 . - pubmed
Considering the remarkably improved device performance , the proposed HTL of Cs0.32WO3 is promising acting as a favorable building block for high efficiency light - emitting devices . - pubmed
ABBREVIATIONS : Continuous Wave : CW ; Code Division Multiple Access : CDMA ; Global System for Mobile Communications : GSM ; Peripheral Blood Mononuclear Cell : PBMC ; Radiofrequency : RF ; Radiofrequency radiation : RFR ; Universal Mobile Telecommunications System : UMTS ; Wideband Code Division Multiple Access : WCDMA ; Specific Absorption Rate : SAR ; National Toxicology Program : NTP ; amplitude - modulated or amplitude - modulation : AM ; Electromagnetic frequencies : EMF ; confidence interval : CI ; Gigahertz : GHz ; odds ratio : OR ; incidence ratio : IR ; reactive oxygen species : ROS ; specific absorption rate : SAR ; International Agency of Research on Cancer : IARC ; single - strand breaks : SSB ; double - strand breaks : DSB ( 7,12 - Dimethylbenz [ a ] anthracene ): DMBA ; Hour : h ; international commission on non - ionizing radiation protection : ICNIRP ; extremely low frequency : ELFl ; microtesla : mT ; Gigahertz : GHz ; hertz : Hz ; decibel : dB ; kilometer : Km ; Watt per square meter : W / m2 ; Hour : h ; positron emission tomography : PET . - pubmed
MSC090213JE08 and its unsaturated fatty acid moiety has been confirmed in vitro to be synthesized by a type II PKS . - pubmed
Biosynthesis of such a highly reduced polyketide by a type II PKS is worthy of note . - pubmed
0.6 ( at pH 7.3 ) under stimulation of both photosystems with a short xenon flash of light . - pubmed
The effect of ribavirin was evaluated by the clinical picture and by the changes in both direct and total serum bilirubin and in the activity of serum glutamic - pyruvic and glutamic - oxaloacetic transaminases . - pubmed
There was a significant decrease in serum bilirubin , SGOT , and SGPT from the 5th to 10th day of treatment in the ribavirin group . - pubmed
[ Action of increasing doses of DL - propranolol on behavior of arterial pressure and plasma renin activity ( PRA ) ] . - pubmed
It may be responsible for a temporary elevation of the LH level in the blood triggering final maturation of the ovarian follicles and an increase of estrogen secretion . - pubmed
An LH - inhibiting activity not related to the negative estrogen feedback is exerted by this nuclear region during critical periods of sexual maturation . - pubmed
Positive inotropic action of hemolysin was observed , and the Na - Ca exchange mechanism was enhanced after hemolysin treatment . - pubmed
The hydrolysis of AA-2 G on the mucosal surface of everted gut was completely inhibited by an alpha - glucosidase inhibitor and the hydrolytic activity of a crude membrane extract diminished to one - forth after immunoprecipitation with the antibody specific to maltase . - pubmed
From these results , it is concluded that ingested AA-2 G serves as a vitamin C source through the hydrolysis by intestinal membrane - bound alpha - glucosidase , mainly maltase , and the subsequent absorption of released ascorbic acid . - pubmed
The proper disk mass for diffusion susceptibility tests with cefoperazone - sulbactam was determined by using a predictor panel of clinical isolates that included staphylococci and gram - negative bacteria intrinsically susceptible , intrinsically resistant , and of various susceptibilities because of the production of different types and amounts of beta - lactamase . - pubmed
Collectively , these results demonstrate that accumulation of plasmalogen catabolites resulting from activation of plasmalogen - selective phospholipases A2 can potentiate alterations in membrane dynamics during signal transduction in plasmalogen - enriched bilayers . - pubmed
We found that both PD + FET and PEP + FET improved whole lung TBC at 30 minutes and 1 h four or fivefold ( p less than 0.01 ) compared with control , whereas at 2 h and 3 h only the improvement following PEP + FET ( approximately 1.4 times ) was significant ( p less than 0.05 ) . - pubmed
There was no significant difference in whole lung or regional TBC between the PD + FET and PEP + FET treatments . - pubmed
A unique stimulation of BBMV ATPase activity occurred at acidic pH in the presence of 1 mM ZnCl2 . - pubmed
In the absence of Zn2 + , a second ATPase activity was stimulated by alkaline pH values with peak stimulation occurring between pH 8.5 and 9.0 . - pubmed
The results suggest that the alkaline pH - stimulated hydrolysis of ATP probably represents the activity of BBMV alkaline phosphatase . - pubmed
The unique acidic pH + Zn2 (+)-stimulated ATPase activity must represent the activity of a second protein other than the alkaline phosphatase , since purified alkaline phosphatase did not show this activity . - pubmed
The deduced IME1 product is a 360 - amino - acid protein with a tyrosine - rich C - terminal region . - pubmed
PGAL1 - IME1 expression did not cause growing cells to sporulate but permitted efficient sporulation of amino acid - limited cells , which otherwise sporulated poorly . - pubmed
To determine whether Sertoli cells and gonocytes are functionally coupled in the cocultures , we used the glass bead - loading technique of McNeil and Warder to introduce Lucifer yellow ( LY ) , a gap junction - permeant probe , and Rhodamine - dextran ( RD ) , a larger marker excluded by gap junctions , simultaneously into cultures 24 h after plating . - pubmed
We have developed two region - specific immunoassays capable of measuring PTHRP in plasma : an immunoradiometric assay directed toward PTHRP amino acid sequence 1 to 74 and a radioimmunoassay directed toward PTHRP amino acid sequence 109 to 138 . - pubmed
The mean ( geometric ) urinary kallikrein activity ( in U / g creatinine ) amounted to 0.52 ( range 0.11 - 1.90 ) in the control group ( n = 193 ) against 0.39 ( range 0.10 - 1.03 ) in the cadmium group , and the prevalence of abnormally low activity levels ( less than or equal to 0.20 U / g creatinine ) amounted to 17.2 % in the cadmium group against 5.2 % in the control group . - pubmed
There was no indication that moderate exposure to mercury vapour ( n = 53 ; mercury in urine , range 11 - 224 micrograms / g creatinine ; average duration of exposure : six years ) or to inorganic lead ( n = 23 ; lead in blood , range 40 - 67 micrograms/100 ml ; average duration of exposure : eight years ) was associated with a reduction of kallikrein production by the kidney . - pubmed
The nutritional requirements of the microorganism must be satisfied and the deleterious effect of oxygen metabolites and of lysosomal hydrolases must be adequately counteracted . - pubmed
Glycoprotein IIb - IIIa is the most prominent Arg - Gly - Asp ( RGD)-binding adhesion receptor on platelets . - pubmed
When an octyl glucoside extract of surface - radioiodinated platelets was applied to an affinity matrix of KYGRGDS - coupled Sepharose 4B , a 160 - kDa - labeled protein ( P160 ) and GPIIb - IIIa bound and were specifically eluted by soluble GRGDSP peptide , but not by the variant GRGESP peptide . - pubmed
Characterization of P160 by two - dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis and by the O'Farrell gel electrophoresis system indicated that P160 is a component of platelet GPIc . - pubmed
This possibility was further substantiated by the complete identity between the determined amino - terminal sequence of P160 and the known sequence of the VnR alpha subunit . - pubmed
The beta 2 - selective antagonist ICI 115881 reversed the effects of epinephrine , whereas the beta 1 - antagonists practolol and betaxolol had little effect . - pubmed
Forskolin , cAMP analogues , and the phosphodiesterase inhibitor 3 - isobutyl-1 - methylxanthine also effectively inhibited ConA - stimulated histamine release . - pubmed
In order to assess the induction of SOD while protecting against the inflammatory process , topical dexamethasone ( dex ) was administered t.i.d . - pubmed
Dex prevented the conjunctival hyperemia , vascular injection and iritis seen with endotoxin treatment alone and blocked the induction of SOD . - pubmed
A similar medrysone ( med ) application ( n = 4 ) failed to prevent the visible signs of ocular inflammation yet also blocked the elevation of SOD . - pubmed
Pretreatment with either of the cyclooxygenase inhibitors , indomethacin ( n = 4 ) or aspirin ( n = 7 ) , failed to block the induction in SOD ( p less than 0.001 and p less than 0.01 , respectively ) . - pubmed
However , the induction of SOD was prevented by the phospholipase A2 inhibitor , quinacrine , and the lipoxygenase inhibitor , nordihydroguaiaretic acid ( NDGA ) . - pubmed
Conformational changes of the Ca2 + - ATPase as early events of Ca2 + release from sarcoplasmic reticulum . - pubmed
The levels of the phosphorylated intermediate ( EP ) and the tryptophan fluorescence of the Ca2 + - ATPase were monitored during both the Ca2 + accumulation and the induced Ca2 + release using fast kinetic techniques . - pubmed
During Ca2 + uptake , both the EP level and the tryptophan fluorescence gradually decreased following a time course similar to that of the Ca2 + accumulation . - pubmed
Upon inducing Ca2 + release by addition of either caffeine or ADP , there was a transient increase of the EP level ( from 0.3 - 0.5 to 1 - 2 nmol / mg protein ) preceding the release of Ca2 + . - pubmed
These results indicate that the Ca2 + - ATPase enzyme undergoes a rapid conformational change in response to triggering of Ca2 + release . - pubmed
In glial cultures treated with 0.25 mM dibutyryl cyclic AMP in the same culture conditions , PST activity is suppressed to about 25 % of its untreated counterpart , even though dibutyryl cyclic AMP at concentrations of ImM only slightly inhibited PST activity in vitro . - pubmed
The effects of Mg2 + ions or EDTA on nuclear integrity and apparent subcellular distribution of unoccupied oestrogen receptors in breast cancer cells . - pubmed
Homogenisation and fractionation of cells in the presence of Mg2 + or EDTA resulted in unoccupied oestrogen receptor being recovered in the particulate fraction . - pubmed
In Mg2 + -prepared fractions , both 5 '- nucleotidase and unoccupied receptor were distributed between plasma membrane , partially - pure nuclei and mitochondrial / microsomal pellets . - pubmed
Mg2 + -prepared nuclei , freed of membranous contamination by pelleting through 1.8 M sucrose , were intact by electron microscopy but had no 5 '- nucleotidase or unoccupied receptor . - pubmed
[ Tyrosinase - oxidoreductase ; monophenol , o - diphenol : O2 ] . - pubmed
USA , in press ): m - xylene , a substrate of the degradative pathway , binds to the xylR gene product ; the m - xylene - xylR product complex activates the xylS gene ; and the xylS product thus synthesized de novo activates the xylDLEGF operon . - pubmed
The authors report that the reversible decrease in calcium , sodium and potassium , resulting from an increase in cerebral lithium levels , can be demonstrated on sampling at 1.30 and 3 hours ( IP ) , or at 12 hours ( VO ) . - pubmed
Phosphorylation of ribosomal protein S6 plateaued at around 2 mol of phosphate / mol of protein with both substrates . - pubmed
Peptide map analyses showed that the most prominent phosphorylation sites associated with 40 S substrates were the adjacent serines in the Arg - Leu - Ser - Ser - Leu - Arg segment of S6 . - pubmed
A larger fragment of S6 ( termed Tlc ) isolated from mild tryptic digests of whole ribosomes , consisted of the T1a sequence extended by the sequence Ser - Glu - Glu - Ser - Gln-(Lys ) at the COOH terminus . - pubmed
Alpha 2 - adrenoceptor - mediated inhibition of electrically evoked [ 3H]noradrenaline release from chick sympathetic neurons : role of cyclic AMP . - pubmed
Both effects of forskolin were potentiated by the phosphodiesterase inhibitor 3 - isobutyl-1 - methylxanthine . - pubmed
The forskolin - induced increase in overflow was abolished by the Rp - diastereomer of cyclic AMP - thioate , an antagonist at cyclic AMP - dependent protein kinases , and 1,9 - dideoxy - forskolin , an inactive analogue at adenylyl cyclase , had no effect on the evoked overflow . - pubmed
A 24 - h pretreatment with either cholera toxin or forskolin reduced the subsequent forskolin - induced accumulation of cyclic AMP and inhibited the stimulation - evoked release . - pubmed
Basal cyclic AMP production , however , remained unaltered after forskolin treatment and was enhanced after 24 h of cholera toxin exposure . - pubmed
Expression levels of the CKS - troponin I fusion were 8 % of total cellular protein 4 h after induction with IPTG . - pubmed
The fusion was affinity - purified over a TnC affinity column using a urea - solubilized extract of a crude cell lysate . - pubmed
TGF - alpha expression was observed in the cells with increased steroidogenesis , including compact tumor cells and zona fasciculata cells with lipid depletion , but did not necessarily correlate with production sites of any specific steroid hormone . - pubmed
Aortic smooth muscle contains guanylate - cyclase - coupled 130 - kDa atrial natriuretic factor receptor as predominant receptor form . - pubmed
The basal guanylate cyclase activity in the aortic membranes was 1.0 pmol cGMP produced.min-1.mg protein-1 at 37 degrees C using Mn ( 2 +)- GTP as substrate . - pubmed
TRH mRNA showed a similar pattern to somatostatin , with a 2.5 - fold increase after 15 min at 4 degrees C. In contrast , hypothalamic TRH content was significantly decreased after 15 min cold exposure , returning to control values at 30 min . - pubmed
Long - term potentiation ( LTP ) of synaptic efficacy in MI horizontal pathways could be produced at short ( 0.5 mm ) and long ( 1.0 mm ) distances by theta burst stimulation ( TBS ) , but only during local , transient application of the gamma - aminobutyric acid - A ( GABAA ) receptor antagonist , bicuculline methiodide ( bic ) immediately before TBS . - pubmed
In animals pretreated with guanethidine , intravenous administration of the vasoactive intestinal polypeptide ( VIP ) receptor antagonist , [ p - Cl - D - Phe6,Leu17 ]- VIP ( 50 micrograms kg-1 ) significantly attenuated the increase in carotid blood flow and decrease in carotid vascular resistance evoked by trigeminal ganglion stimulation . - pubmed
Responses evoked by trigeminal ganglion stimulation were , however , unaffected by intravenous injection of the tachykinin NK1 receptor antagonists , GR82334 ( 0.3 mg kg-1 ) and CP-99,994 ( 0.4 mg kg-1 ) , calcitonin gene - related peptide ( CGRP ) receptor antagonist , CGRP8 - 37 ( 0.9 mg kg-1 ) and the ganglion blocking agent , hexamethonium ( 10 mg kg-1 ) . - pubmed
Development and characterization of hydrogen peroxide - resistant Chinese hamster ovary ( CHO ) cell variants -- II . - pubmed
Further experiments have demonstrated that , under conditions of reduced NPSH content ( obtained by growing the cells in the presence of a glutamylcysteine synthetase inhibitor ) , the cytotoxic action of hydrogen peroxide was very slightly , if at all , augmented in both wild type and resistant cells . - pubmed
Remarkably , a previously unreported deficient variant , G6PD Orissa ( 44 Ala-->Gly ) , is responsible for most of the G6PD deficiency in tribal Indian populations but is not found in urban populations , where most of the G6PD deficiency is due to the G6PD Mediterranean ( 188 Ser-->Phe ) variant . - pubmed
We have shown that a PI3 K product , phosphatidylinositol ( PI ) 3,4,5 - trisphosphate , promotes pleckstrin phosphorylation in platelets . - pubmed
Since pleckstrin homology domains are thought to interact with Gbetagamma heterodimers and/or PI ( 4,5 ) P2 , we have examined the effects of recombinant pleckstrins on platelet PI3Kgamma and p85 / PI3 K activities . - pubmed
Pleckstrin - mediated inhibition of PI3Kgamma is overcome by excess Gbetagamma and is restricted to PI ( 4,5 ) P2 as substrate , i.e. - pubmed
Consistent with this , activation of protein kinase C by exposure of platelets to beta - phorbol diester ( to increase endogenous pleckstrin phosphorylation ) prior to platelet lysis causes inhibition of Gbetagamma - stimulatable PI3 K activity only with respect to PI ( 4,5 ) P2 substrate . - pubmed
MK-801 increased CGRP in a dose - response manner in frontal cortex , and NKA - LI in occipital cortex . - pubmed
Although the higher MK-801 dose reduced seizure duration by 50 % , the ECS induced NPY - LI increase in striatum , occipital cortex and hippocampus , and NKA - LI in occipital cortex was not diminished . - pubmed
Consistent with these findings , thalidomide ( which antagonizes TNF action ) attenuates c - src induction by LPS . - pubmed
This concept was supported through histologic analysis of hematoxylin- and eosin - stained sections , as well as by the pattern of immunohistochemical reactivity with antibodies directed against low and high molecular weight cytokeratins , cell adhesion molecules ( CAM 5.2 ) , and carcinoembryonic antigens . - pubmed
The percentage of hearing loss calculated for both ears , using CPT index , demonstrated the highest values in the group of subjects drinking non - consumable alcohols and addicted to volatile organic substances . - pubmed
Progesterone and transforming growth factor - beta coordinately regulate suppression of endometrial matrix metalloproteinases in a model of experimental endometriosis . - pubmed
Our findings indicate that blocking the action of TGF - beta opposes progesterone - mediated suppression of MMPs and blocks the ability of this steroid to prevent experimental endometriosis . - pubmed
However , we also show that the action of TGF - beta does not lead to a sustained suppression of MMPs as observed following progesterone treatment . - pubmed
Taken together , our data suggest that in the absence of a normal progesterone response , common in ectopic lesions of endometriosis , sensitivity to TGF - beta may be altered , resulting in a failure to regulate MMPs . - pubmed
A7 , A8 , A18 and A19 , respectively , were cured and found to encode at least part of the metabolic pathway involved in the growth on ortho - chlorinated benzoates . - pubmed
These data indicate that TNFR1 and CD95 are dispensable for deletion of antigen - specific T cells after viral infection . - pubmed
The DPD activity of pancreatic tumors may be a useful clinical marker of the responsibility of fluorinated pyrimidine dosing in pancreatic cancer . - pubmed
ATP - stimulated release of interleukin ( IL )- 1beta and IL-18 requires priming by lipopolysaccharide and is independent of caspase-1 cleavage . - pubmed
Monocytes rapidly processed and released both IL-1beta and IL-18 after exogenous ATP . - pubmed
Despite its constitutive cytosolic presence , IL-18 required lipopolysaccharide priming for the ATP - induced release . - pubmed
Release of both cytokines was blocked completely by a P2X7 receptor antagonist , oxidized ATP , and partially by an antibody to P2X ( 7 ) receptor . - pubmed
In evaluating the signaling components involved in the ATP effect , we identified that the protein - tyrosine kinase inhibitor , AG126 , produced a profound inhibition of both ICE activation as well as release of IL-1beta / IL-18 . - pubmed
Taken together , these results suggest that , although synthesis of IL-1beta and IL-18 differ , ATP - mediated release of both cytokines requires a priming step but not proteolytically functional caspase-1 . - pubmed
Interaction of the herbicide glyphosate with its target enzyme 5 - enolpyruvylshikimate 3 - phosphate synthase in atomic detail . - pubmed
We have identified the interaction of EPSP synthase with one of its two substrates ( shikimate 3 - phosphate ) and with the widely used herbicide glyphosate by x - ray crystallography . - pubmed
Glyphosate appears to occupy the binding site of the second substrate of EPSP synthase ( phosphoenol pyruvate ) , mimicking an intermediate state of the ternary enzyme.substrates complex . - pubmed
The elucidation of the active site of EPSP synthase and especially of the binding pattern of glyphosate provides a valuable roadmap for engineering new herbicides and herbicide - resistant crops , as well as new antibiotic and antiparasitic drugs . - pubmed
The discs large ( Dlg ) protein , or synapse - associated protein 97 ( SAP97 ) , is a member of the membrane - associated guanylate kinase family of multidomain scaffolding proteins which recruits transmembrane and signaling molecules to localized plasma membrane sites . - pubmed
BACKGROUND : The occurrence of phase III of migrating motor complexes in neonates is inversely related to gestational age , and it can be triggered in some infants by the motilin receptor agonist erythromycin . - pubmed
Immunoreactivity of SP is enhanced after acute chemical stimulation of somata . - pubmed
This enhancement was significantly attenuated by tamsulosin in the L6 and S1 segments . - pubmed
Tamsulosin can attenuate nociception - induced SP upregulation within the spinal cord . - pubmed
The black women , however , had lower plasma triglyceride levels ( 0.91 + -0.46 vs. 1.22 + -0.60 mmol / L , p<0.0001 ) and a trend toward higher high - density lipoprotein cholesterol levels ( 1.37 + -0.34 vs. 1.29 + -0.31 mmol / L , p=0.064 ) than the white women . - pubmed
Plasma total and low - density lipoprotein cholesterol levels were similar . - pubmed
In summary , compared to white women , black women had a higher mean body mass index , higher blood pressures , higher lipoprotein ( a ) and plasma total homocysteine levels , and greater consumption of saturated fat and cholesterol . - pubmed
In order to facilitate further studies on the molecular function of OMP , its crystal structure has been determined at 2.3 A resolution using multiwavelength anomalous diffraction experiments on selenium - labeled protein . - pubmed
Concentrations of 50 and 100 microM of this compound increased laccase activity when compared with control samples grown without tannic acid . - pubmed
The role of tannic acid was shown to be an inductor of laccase activity in this fungus , due to the enhancement of expression of the laccase gene at the transcriptional level . - pubmed
At 3 months , she developed hypocalcemia and hyperphosphatemia with normal levels of vitamin D and alkaline phosphatase , and parathyroid hormone level was low . - pubmed
DECREASED GLUTATHIONE IN AGING RED CELLS . - pubmed
The role of Ca ( 2 + ) ions in tubular toxicity and the effect of calcium channel blockers on CsA toxicity were evaluated by measuring intracellular calcium using the fluorescent dye Fura-2 AM . - pubmed
After 90 min incubation , LDH was 22.5 % in control PT and 41.9 % in PT treated with 100 micro g / ml CsA ( P & lt ; 0.001 , n = 11 ) . - pubmed
Both treatments significantly lowered triglycerides and increased high - density lipoprotein ( HDL ) cholesterol levels from baseline ; however , these changes were not significantly different between treatments . - pubmed
Low - density lipoprotein ( LDL ) cholesterol concentrations increased significantly in subjects who consumed DHA - enriched eggs ( p = 0.047 vs. control ) . - pubmed
However , there was no significant increase in cholesterol carried by small , dense LDL particles , as determined by nuclear magnetic resonance analysis . - pubmed
Results of exploratory analyses suggest favorable effects of the DHA - enriched eggs over ordinary eggs on triglyceride and HDL cholesterol levels in subjects with body mass index & gt ; or = 30 kg / m2 ; the DHA treatment produced a larger reduction in serum triglyceride concentration vs. ordinary eggs ( -12.3 vs. 2.1 % ; p = 0.027 ) , and there was a greater increase for HDL cholesterol in the DHA - enriched vs. ordinary egg group ( 5.0 vs. 1.1 % ; p = 0.040 ) . - pubmed
1.0 % ) , and beta - bourbonene ( 0.6 - 1.5 % ) , in the HD , SDE , MWHD , and SFE volatile fractions . - pubmed
Minute ventilation ( VE ) , respiratory rate ( RR ) , endtidal CO ( 2 ) pressure ( P(ECO(2 ) ) , and arterial hemoglobin oxygen saturation ( SpO2 ) were measured with a facemask tightly fitted before and during 7 % CO ( 2 ) inhalation . - pubmed
This study examined the effect of vegetarianism on skeletal muscle total creatine ( TCr ) content and creatine transporter ( CreaT ) gene expression , prior to and during 5 d of Cr supplementation ( CrS ) . - pubmed
OBJECTIVES : Previously , we reported that etoposide oleate associated to a cholesterol - rich microemulsion ( LDE ) is taken up by malignant cells overexpressing low - density lipoprotein ( LDL ) receptors . - pubmed
There was no difference in silica exposure between patients with cytoplasmic ANCA ( c - ANCA ) , perinuclear ANCA ( p - ANCA ) , or atypical ANCA . - pubmed
Skeletal muscle expresses two catalytic subunits , alpha1 and alpha2 , of the 5 '- AMP - activated protein kinase ( AMPK ) , which has been implicated in contraction - stimulated glucose transport and fatty acid oxidation . - pubmed
AMPK activation was associated with increased phosphorylation of Thr(172 ) of the alpha - subunit , the primary activation site . - pubmed
Muscle stimulation increased the phosphorylation of acetyl - CoA carboxylase ( ACC ) , a downstream target of AMPK , and the rate of 3 - O - methyl - d - glucose transport . - pubmed
In contrast , increasing the frequency ( & gt;or=5 Hz ) or duration ( & gt;or=5 min ) of contraction activated AMPKalpha1 and AMPKalpha2 and increased AMP concentration and the AMP / ATP ratio . - pubmed
These results suggest that 1 ) AMPKalpha1 is the predominant isoform activated by AMP - independent phosphorylation in low - intensity contracting muscle , 2 ) AMPKalpha2 is activated by an AMP - dependent mechanism in high - intensity contracting muscle , and 3 ) activation of each isoform enhances glucose transport and ACC phosphorylation in skeletal muscle . - pubmed
NO-1886 also suppressed fat accumulation in visceral ( 46.9 + /- 10.4 vs 73.7 + /- 14.5 g , P & lt ; .01 ) and subcutaneous ( 43.1 + /- 18.1 vs 68.9 + /- 18.8 g , P & lt ; .05 ) tissues and increased the levels of plasma total cholesterol and high - density lipoprotein cholesterol in comparison to the high - fat control group . - pubmed
In contrast , NO-1886 decreased the levels of plasma triglycerides , nonesterified free fatty acid , glucose , and insulin . - pubmed
NO-1886 increased LPL activity in soleus muscle ( 0.082 + /- 0.013 vs 0.061 + /- 0.016 mumol of free fatty acid per minute per gram of tissue , P & lt ; .05 ) . - pubmed
NO-1886 increased the expression of UCP3 mRNA in soleus muscle 3.14 - fold ( P & lt ; .01 ) compared with the high - fat control group without affecting the levels of UCP3 in mesenteric adipose tissue and BAT . - pubmed
In addition , NO-1886 did not affect the expression of UCP1 and UCP2 in BAT , mesenteric adipose tissue , and soleus muscle . - pubmed
In conclusion , NO-1886 increased the expression of UCP3 mRNA and LPL activity only in skeletal muscle . - pubmed
High resolution electronic spectra of anisole and anisole - water in the gas phase : hydrogen bond switching in the S1 state . - pubmed
Our results indicate that a decrease in P700 activity ( with a resultant increase in P700 unit size ) may be partially responsible for the photoinhibition of algal photosynthetic carbon dioxide fixation . - pubmed
These isozymes were partially purified on a DEAE - Trisacryl column . - pubmed
Comparison of C40/82A and P27A C40/82A barstar mutants using 19F NMR . - pubmed
Barstar , an inhibitor of the enzyme barnase , contains two phenylalanine residues , three tryptophan residues , and two proline residues . - pubmed
Barstar is cold denatured in the presence of urea . - pubmed
BACKGROUND : The ABCC5 gene encodes an organic anion pump of the ATP - binding cassette ( ABC ) transporter family , subclass C. The exact physiological function of ABCC5 however is not known . - pubmed
Ca ( 2 + ) binding increases the alpha - helix content and causes significant thermal stabilization with a melting temperature increase of approximately 10 degrees C. Functional Ca ( 2 +)-dependent phospholipid binding sites of annexin B1 were investigated using mutant proteins . - pubmed
By changing a conserved acidic amino acid residue that putatively combines Ca ( 2 + ) in each domain of annexin B1 singly or in combination , we found that Ca ( 2 + ) binding in the first domain is more important than that at the other Ca ( 2 + ) binding sites . - pubmed
The effectiveness and safety of topical PhotoActif phosphatidylcholine - based anti - cellulite gel and LED ( red and near - infrared ) light on Grade II - III thigh cellulite : a randomized , double - blinded study . - pubmed
CONCLUSIONS : The results of this small but well - documented , randomized , double - blinded study affirms that eight of nine thighs with Grade II - III cellulite responded positively to a novel , combined 3 - month treatment program of a phosphatidylcholine - based , anti - cellulite gel and LED exposure , as determined by the clinical determinants obtained . - pubmed
Severe overresponders and underresponders to upshock were further scrutinized for their calcium content with ( 45 ) Ca and their Yvc1 electrophysiological activities under patch - clamp . - pubmed
The severe underresponders have lower calcium content but no change in Yvc1 activity . - pubmed
Erythritol ( INS 968 ) is an important four - carbon sugar alcohol in the food industry . - pubmed
Currently , HPLC and GC methods are in use for the quantification of erythritol in natural / processed foods . - pubmed
We have utilized affinity - purified erythritol - specific antibodies generated earlier [ 9 ] to develop an indirect competitive ELISA . - pubmed
With erythritol - BSA conjugate ( 54 mol / mol ; 100 ng / well ) as the coating antigen , a calibration curve was prepared using known amounts of standard meso - erythritol ( 0.1 - 100,000 ng ) in the immunoassay . - pubmed
In contrast , all erythromycin - resistant isolates ( 73 ) were either constitutively resistant to telithromycin ( 13 isolates with constitutive erm genes ) or demonstrated telithromycin D - shaped zone ( 60 isolates with inducible msr(A ) and/or erm ) . - pubmed
All these isolates were resistant to streptogramin A and harbored vga ( A ) gene ( 1 isolate ) or vga ( A ) LC gene ( 19 isolates ) . - pubmed
Thiazide diuretics remain primary first - step agents , especially for uncomplicated hypertension ; calcium channel blockers are also efficacious . - pubmed
The supernatant is removed and 2 ml of Neural Basal Media ( NBM ) containing 2 % B27 ( vol / vol ) and 0.25 % Glutamax ( vol / vol ) is added to the cortex which is then re - suspended by pipetting up and down . - pubmed
The supernatant is then removed and the cortex pellet re - suspended with 2 ml of NBM containing B27 and Glutamax . - pubmed
Furthermore , a single point mutation in the CD28 cytosolic tail ( tyrosine 188 ) interferes with the ability of CD28 to preferentially accumulate at the cSMAC . - pubmed
PKCtheta distribution at the immunological synapse mirrors the distribution of tyrosine 188 - mutated CD28 , indicating that CD28 drives the localization of PKCtheta even when CD28 is not localized to the cSMAC . - pubmed
Mutation of tyrosine 188 also results in diminished activation of NF - kappaB , suggesting that CD28 - mediated localization of PKCtheta to the cSMAC is important for efficient signal transduction . - pubmed
The binding of the antigen - antibody complex was visualized by the addition of commercial rabbit antisheep IgG antibody labeled with alkaline phosphatase with subsequent addition of p - nitrophenyl phosphate substrate to produce a colored product for quantification . - pubmed
We also briefly illustrate three established methods to assess HPA axis activity , reactivity , and feedback functioning , namely the cortisol awakening rise ( CAR ) , Trier Social Stress Test ( TSST ) , and low - dose Dexamethasone Suppression Test ( DST ) . - pubmed
This intron - less gene is 1,149 - base pair long encoding 382 amino acids - putative membrane - bound Delta12 desaturase protein . - pubmed
The signature amino acid sequence ' YNNKL ' was also found to be present at the N terminus of the protein , which is necessary and sufficient for ER localization of enzyme . - pubmed
However , exogenously supplied linoleic acid was feebly converted to linolenic acid suggesting that LuFAD2 - 2 encodes a functional FAD2 enzyme and has substrate specificity similar to LuFAD2 . - pubmed
Plasma ADPN levels were directly correlated with age , HDL - C and creatinine , and inversely with BMI , triglycerides and glomerular filtration rate ( GFR ) . - pubmed
Multiple regression analysis identified plasma creatinine and HDL as the independent factors associated with ADPN plasma levels . - pubmed
There were statistically significant reductions in plasma total cholesterol , low - density lipoprotein cholesterol and triglyceride concentrations after dietary intervention . - pubmed
Multiple linear regression analyses showed that the AC and CC CYP7A1 genotypes accounted for 5.2 and 6.2 % of triglyceride concentration during follow - up and adjusted percent of change of triglyceride concentration , respectively . - pubmed
The present study provides evidence that -278A>C polymorphism in the CYP7A1 gene can modify triglyceride concentrations in response to a reduced fat diet in a dyslipidemic male population . - pubmed
Donepezil , an acetylcholinesterase inhibitor , is an approved drug for the treatment of Alzheimer ' s disease ( AD ) . - pubmed
Multiple linear regression and artificial neural networks ( ANNs ) as feature mapping techniques were used for the prediction of the biomagnification factor ( BMF ) of some organochlorine pollutants . - pubmed
Thus , more than 80 % patients with prior failure to nonnucleoside reverse transcriptase inhibitors could potentially benefit from etravirine rescue therapy . - pubmed
No difference in insulin sensitivity , measured as the glucose infusion rate necessary to maintain euglycaemia , was observed . - pubmed
Self - assembly on phosphoinositide - containing membranes stimulates dynamin GTPase activity , which is crucial for its function . - pubmed
Although the pleckstrin - homology ( PH ) domain is known to mediate phosphoinositide binding by dynamin , it remains unclear how this promotes activation . - pubmed
Here , we describe studies of dynamin PH domain mutations found in centronuclear myopathy ( CNM ) that increase dynamin 's GTPase activity without altering phosphoinositide binding . - pubmed
CNM mutations in the PH domain C - terminal alpha - helix appear to cause conformational changes in dynamin that alter control of the GTP hydrolysis cycle . - pubmed
Potent antifibrotic activity of mTOR inhibitors sirolimus and everolimus but not of cyclosporine A and tacrolimus in experimental liver fibrosis . - pubmed
CONCLUSIONS : mTOR inhibition by sirolimus and everolimus in experimental liver fibrosis associates with significantly less fibrosis progression and portal hypertension than treatment with calcineurin inhibitors tacrolimus and cyclosporine A. - pubmed
The day trip to the forest park significantly reduced blood pressure and urinary noradrenaline and dopamine levels and significantly increased serum adiponectin and dehydroepiandrosterone sulfate ( DHEA - S ) levels . - pubmed
Walking exercise also reduced the levels of serum N - terminal pro - B - type natriuretic peptide ( NT - proBNP ) and urinary dopamine . - pubmed
Taken together , habitual walking in forest environments may lower blood pressure by reducing sympathetic nerve activity and have beneficial effects on blood adiponectin and DHEA - S levels , and habitual walking exercise may have beneficial effects on blood NT - proBNP levels . - pubmed
Pre- and post - tests of server responses to pregnant - appearing " pseudo - patron " actors ordering alcohol in experimental ( n = 148 ) and comparison ( n = 183 ) establishments were a key method of evaluating the efficacy of this intervention . - pubmed
It occurs through two different mechanisms : Ca ( 2 + ) release from internal stores , involving phospholipase C ( PLC)-dependent generation of inositol-1,4,5 - trisphosphate ( IP3 ) and activation of IP3 sensitive channels in the store membrane , and Ca ( 2 + ) influx across the plasma membrane . - pubmed
In the last years , however , the Ca ( 2 + ) sensor stromal interaction molecule 1 ( STIM1 ) and the channel protein Orai1 emerged as the key players in platelet SOCE . - pubmed
Low - molecular mass proteins rich in cysteines called metallothioneins ( MT ) can be considered as markers for the pollution of the environment by metals . - pubmed
Light triggered detection of aminophenyl phosphate with a quantum dot based enzyme electrode . - pubmed
pAPP as substrate is converted by the enzyme alkaline phosphatase ( ALP ) to generate 4AP as a product . - pubmed
We hypothesized that doxycycline , a broad MMPs inhibitor , was as effective as simvastatin in reducing the incidence of plaque disruption . - pubmed
The results showed that oral administration of doxycycline resulted in a significant increase in the thickness of the fibrous cap of the aortic plaque whereas there was a substantial reduction of MMPs expression , local and systemic inflammation , and aortic plaque vulnerability . - pubmed
Both the number and localization of granzyme B - positive cells were altered by vitamin A. - pubmed
Other cytokines were reduced and chemokines increased as the disease progressed , but no additional effects of vitamin A were seen . - pubmed
Fragments added to the de novo derivatives 3a , 3b , 3c for GABA binding site , and 1a , 2a , and 3d for BZ binding site contributed to new binding sites and structural stability , further optimizing binding to GABA or BZ binding sites . - pubmed
Derivative structures can be used as starting points for developing high affinity GABAA receptor agonists with specificity towards GABA binding site and BZ binding site . - pubmed
Given the impact of amphipathic small molecules on bilayer properties such as elasticity and intrinsic curvature , we investigated the effects of six commonly used PAs -- cyclooctatetraene ( COT ) , para - nitrobenzyl alcohol ( NBA ) , Trolox ( TX ) , 1,4 - diazabicyclo [ 2.2.2 ] octane ( DABCO ) , para - nitrobenzoic acid ( pNBA ) , and n - propyl gallate ( nPG)--on bilayer properties using a gramicidin A ( gA)-based fluorescence quench assay to probe for PA - induced changes in the gramicidin monomer dimer equilibrium . - pubmed
, PA adsorption at the bilayer / solution interface that alter the equilibrium constant for gA channel formation , and thus the number of conducting gA channels in the large unilamellar vesicle membrane , will be detectable as changes in the rate of Tl + influx - the fluorescence quench rate . - pubmed
Ambient ultrafine particles reduce endothelial nitric oxide production via S - glutathionylation of eNOS . - pubmed
Here , we tested whether ambient ultrafine particles ( UFP , diameter & lt;200 nm ) modulate eNOS activity in terms of nitric oxide ( NO ) production via protein S - glutathionylation . - pubmed
UFP - mediated reduction in NO production was restored in the presence of JNK inhibitor ( SP600125 ) , NADPH oxidase inhibitor ( Apocynin ) , anti - oxidant ( N - acetyl cysteine ) , and superoxide dismutase mimetics ( Tempol and MnTMPyP ) . - pubmed
UFP exposure increased the GSSG / GSH ratio and eNOS S - glutathionylation , whereas over - expression of Glutaredoxin-1 ( to inhibit S - glutathionylation ) restored UFP - mediated reduction in NO production by nearly 80 % . - pubmed
Thus , our findings suggest that eNOS S - glutathionylation is a potential mechanism underlying ambient UFP - induced reduction of NO production . - pubmed
Carbon dioxide as a C1 building block for the formation of carboxylic acids by formal catalytic hydrocarboxylation . - pubmed
The concentrations of FX and NIV in the plasma and various tissues were measured using LC - MS / MS . - pubmed
FX and NIV were detectable in the vital organs up to 24h after po administration . - pubmed
In vitro of liver postmitochondrial fractions with FX demonstrated that the liver and kidney are capable of FX - to - NIV metabolism . - pubmed
In this work , we successfully synthesized a large area of highly - crystalline MoSe2 atomic layers on SiO2 / Si , mica and Si substrates using a simple chemical vapour deposition ( CVD ) method at atmospheric pressure . - pubmed
Atomic force microscopy ( AFM ) and Raman spectroscopy reveal that the as - grown ultrathin MoSe2 layers change from a single layer to a few layers . - pubmed
Photoluminescence ( PL ) spectroscopy demonstrates that while the multi - layer MoSe2 shows weak emission peaks , the monolayer has a much stronger emission peak at ~ 1.56 eV , indicating the transition from an indirect to a direct bandgap . - pubmed
Transmission electron microscopy ( TEM ) analysis confirms the single - crystallinity of MoSe2 layers with a hexagonal structure . - pubmed
Kirsten rat sarcoma viral oncogene ( KRAS ) mutations make some tumours less responsive to treatment with biological therapies such as cetuximab . - pubmed
OBJECTIVES : To compare the performance and cost - effectiveness of KRAS mutation tests in differentiating adults with metastatic colorectal cancer whose metastases are confined to the liver and are unresectable and who may benefit from first - line treatment with cetuximab in combination with standard chemotherapy from those who should receive standard chemotherapy alone . - pubmed
Two studies provided data on the accuracy of KRAS mutation testing for predicting response to treatment in patients treated with cetuximab plus standard chemotherapy . - pubmed
Four RCTs provided data on the clinical effectiveness of cetuximab plus standard chemotherapy compared with that of standard chemotherapy in patients with KRAS wild - type tumours . - pubmed
Glufosinate ammonium dissipated rapidly in soil to MPA in hours and gradually degraded to MPP . - pubmed
Poly ( epsilon - caprolactone ) nanofibers were surface modified with mussel inspired DOPA - melanin ( DM ) coating for adsorption of REST siRNA . - pubmed
It was found that the extent of accumulation of Ca , P , Zn , and Na was not uniform throughout the LAD artery and was higher in the proximal part than in the distal part with regard to the LAD artery beneath the MB ( the tunneled LAD artery ) . - pubmed
The extent of accumulation of Ca , P , Zn , and Na in the proximal part of the tunneled LAD artery was similar to that in the segments proximal to the MB , whereas the extent of accumulation of Ca , P , Zn , and Na in the distal part of the tunneled LAD artery was similar to that in the segments distal to the MB . - pubmed
We also identify a downregulation of miR-150 in CD46 - costimulated T cells and identify the glucose transporter 1 encoding transcript SLC2A1 as a target of miR-150 regulation , connecting miR-150 with modulation of glucose uptake . - pubmed
OBJECTIVES : To evaluate the relevance of anti - adalimumab ( anti - ADA ) antibodies ( Abs ) and their relationship with clinical / laboratory features in rheumatoid arthritis ( RA ) , ankylosing spondylitis ( AS ) and psoriatic arthritis ( PsA ) . - pubmed
Clinical / laboratory characteristics , disease activity , anti - ADA , anti - nuclear ( ANA ) , anti - double strand ( ds)DNA , anti - extractable nuclear antigens ( anti - ENA ) and anti - phospholipid Abs ( aPL ) were evaluated at baseline , 4 , 12 and 24 weeks of adalimumab treatment . - pubmed
Anti - ADA positivity was associated with significantly lower mean adalimumab serum levels ( P<0.05 ) . - pubmed
CONCLUSIONS : Anti - ADA abs could be considered an early marker associated to a poor clinical response to adalimumab treatment . - pubmed
NJE effectively inhibited 2,2 '- azobis ( 2 - methylpropionamidine ) dihydrochloride ( AAPH)-induced oxidation of biomolecules analyzed by pBR322 plasmid DNA damage , protein oxidation of bovine serum albumin and lipid peroxidation assays . - pubmed
The placenta growth factor ( PlGF ) is a member of the vascular endothelial growth factor ( VEGF ) family , which shares with VEGF - A the tyrosine kinase receptor VEGFR-1 and the co - receptor neuropilin-1 ( NRP-1 ) . - pubmed
Our findings emphasize the pivotal relevance of RNAi as an effective tool for increasing the therapeutic efficacy of PMX , a cornerstone in the treatment regimens of MPM , and thereby , raising the possibility for the development of a novel therapeutic strategy , combination therapy of TS - shRNA and PMX , that can surpass many of the currently applied , but less effective , therapeutic regimens against lethal MPM . - pubmed
Furthermore various hormones , including leptin and cortisone , are determined in cord plasma , and in heel prick blood spots ( thyroxine ) . - pubmed
Compared with the intakes of healthy children from National Nutritional Survey , the intake of IBD group was lower for calories ( 78 % , P = 0.012 ) , carbohydrates ( 61 % P & lt ; 0.05 ) , magnesium ( 67 % P & lt ; 0.05 ) , vitamin C ( 34 % , P & lt ; 0.05 ) , and fiber ( 54 % , P & lt ; 0.05 ) and high for B12 ( 141 % , P & lt ; 0.05 ) . - pubmed
After AVP - Vl receptor antagonist ( [ Deamino - penl , val4 , D - Arg8 ]- vasopressin ) was injected into NTS or bilateral chemical damage to NTS , the protective effect of the chemical stimulation of PVN on UC was also eliminated . - pubmed
Momelotinib in myelofibrosis : JAK1 / 2 inhibitor with a role in treating and understanding the anemia . - pubmed
On MR , it was hypointense on T1 , hyperintense on T2 , and did not enhance with gadolinium . - pubmed
ERCC1 and ERCC2 as predictive biomarkers to oxaliplatin - based chemotherapy in colorectal cancer patients from Egypt . - pubmed
BACKGROUND : The impact of Excision repair cross - complementation group 1 ( ERCC1 ) and group 2 ( ERCC2 ) expression levels on the efficacy of oxaliplatin - based chemotherapy is still controversial . - pubmed
The present study was conducted to determine the predictive value of these molecular biomarkers in stage III and IV colorectal cancer ( CRC ) patients receiving oxaliplatin ( OX)-based chemotherapy as first - line treatment . - pubmed
CONCLUSIONS : ERCC1 expression levels may help in selecting patients who benefit from oxaliplatin chemotherapy in stage III & amp ; IV CRC . - pubmed
The clinical resolution rates in the PPS population at the test of cure ( TOC , primary efficacy end point , 7 - 30 days post - therapy ) visit were 96.86 % ( 154/159 ) for morinidazole and 96.73 % ( 148/153 ) for ornidazole ( 95 % CI : -3.79 % to 4.03 % ) . - pubmed
Vitamin D across growth hormone ( GH ) disorders : From GH deficiency to GH excess . - pubmed
The interplay between vitamin D and the growth hormone ( GH )/ insulin - like growth factor ( IGF )- I system is very complex and to date it is not fully understood . - pubmed
GH directly regulates renal 1 alpha - hydroxylase activity , although the action of GH in modulating vitamin D metabolism may also be IGF - I mediated . - pubmed
On the other hand , vitamin D increases circulating IGF - I and the vitamin D deficiency should be normalized before measurement of IGF - I concentrations to obtain reliable and unbiased IGF - I values . - pubmed
Indeed , linear growth after treatment of nutritional vitamin D deficiency seems to be mediated through activation of the GH / IGF - I axis and it suggests an important role of vitamin D as a link between the proliferating cartilage cells of the growth plate and GH / IGF - I secretion . - pubmed
Conversely , in active acromegaly , a condition characterized by a chronic GH excess , both increased and decreased vitamin D levels have been highlighted , and the interplay between vitamin D and the GH / IGF - I axis becomes even more complicated when we consider the acromegaly treatment , both medical and surgical . - pubmed
The current review summarizes the available data on vitamin D in the main disorders of the GH / IGF - I axis , providing an overview of the current state of the art . - pubmed
Two types of SAMs with different dipole direction , thiophenol ( TP ) and pentafluorobenzene thiol ( PFBT ) were employed , modifying the work function of the inkjet - printed Ag ( 4.9 eV ) to 4.66 eV and 5.24 eV with TP and PFBT treatments , respectively . - pubmed
The analytes were completely separated from the endogenous compounds on an Agilent Poroshell 120 SB - C18 column ( 4.6mmx150 mm , 2.7mum ) using an isocratic elution with methanol - 0.1 % formic acid aqueous ( 4/1 , v / v ) as a mobile phase . - pubmed
Positron emission tomography computed tomography ( PET - CT ) scan of the lymph nodes revealed abnormal uptake of fluorodeoxyglucose ( FDG ) . - pubmed
The use of MAPT was associated with similar mortality rate ( 5.9 % vs. 6.6 % ; RR = 0.92 ; 95 % CI 0.49 - 1.71 ; P = 0.68 ) and stroke rate compared with DAPT ( 1.3 % vs. 1.3 % ; RR 1.04 ; 95 % CI 0.27 to 4.04 ; P = 0.81 ) . - pubmed
CONCLUSION : MAPT use after TAVR is associated with lower rates of major bleeding compared with DAPT with no significant difference in mortality , stroke or vascular complications . - pubmed
[ Effects of pargyline on histone methylation in promoter and enhancer regions and transcription of CYP3A4/3A7 ] . - pubmed
This study was designed to investigate effects of pargyline on histone methylation in the promoter and enhancer regions and transcription of cytochrome P450 3A4 / 3A7 ( CYP3A4/3A7 ) gene . - pubmed
The chromatin immunoprecipitation ( ChIP ) assay was performed with dimethylation of histone H3 at lysine 4 ( H3K4me2 ) , and IgG antibodies respectively . - pubmed
In conclusion , the enriched H3K4me2 in the promoter and enhancer regions was induced by pargyline with HNF4A or GR binding site in CYP3A4/3A7 gene to activate the corresponding genes . - pubmed
The total nitrogen ( TN ) content in soil is an important index for soil fertility and the rapid prediction of TN content with spectroscopy serves an important function in precision agriculture . - pubmed
However , whether the TN content in soil is predicted through its relationship with soil organic carbon ( SOC ) or on its specific absorption is still debatable . - pubmed
In order to explore the mechanism of reflectance spectroscopy for predicting total nitrogen in soil , the changes of model accuracies and the correlation of TN and SOC were analyzed . - pubmed
Tacrolimus and Single Intraoperative High - dose of Anti - T - lymphocyte Globulins Versus Tacrolimus Monotherapy in Adult Liver Transplantation : One - year Results of an Investigator - driven Randomized Controlled Trial . - pubmed
These excess Mo atoms can then reorganize into closed , triangular MTB - loops that can grow in size by adding more Mo atoms to them . - pubmed
Such MTB networks have been observed in samples grown by molecular beam epitaxy ( MBE ) and consequently their presence needs to be considered in understanding the properties of MBE grown MoSe2 and MoTe2 . - pubmed
INTRODUCTION : Once - weekly semaglutide is a novel glucagon - like peptide-1 ( GLP-1 ) analogue for the treatment of type 2 diabetes that was associated with greater reductions in glycated hemoglobin ( HbA1c ) and body mass index ( BMI ) versus once - daily GLP-1 analogue liraglutide in a recent network meta - analysis ( NMA ) . - pubmed
Influence of nonsynaptic alpha1 glycine receptors on ethanol consumption and place preference . - pubmed
We examined whether intraoperative regional cerebral oxygen saturation ( rSO2 ) and neurological dysfunction prior to surgery , measured by robotic technology , are important predictors of post - operative performance following CABG surgery . - pubmed
DISCUSSION : Our results suggest that pre - operative performance is a stronger indicator of post - operative neurological outcome than intraoperative rSO2 , and should be included as an important variable when elucidating the relationship between cerebral oxygen levels and post - operative neurological impairment . - pubmed
We found that those compounds slightly altered the expression of hNSC identity markers ( SOX2 , SOX3 and NES ) , without impairing cell viability or proliferation , in part by either modulating glycogen synthase kinase 3 beta ( GSK3beta ) signaling ( TBBPS , TCBPA and BDE-47 ) , and slightly disturbing the NOTCH pathway ( TBBPA , TBBPS and TCBPA ) . - pubmed
Pinholin S2168 is a 68 amino acid integral membrane protein consisting of two transmembrane domains ( TMDs ) called TMD1 and TMD2 . - pubmed
Systematic site - directed spin labeling and continuous wave electron paramagnetic resonance ( CW - EPR ) spectroscopic studies of pinholin S2168 in 1,2 - dimyristoyl - sn - glycero-3 - phosphocholine ( DMPC ) proteoliposomes are used to reveal the structural topology and dynamic properties in a native - like environment . - pubmed
Fabclavines are NRPS - derived hexapeptides , which are connected by PKS - derived malonate units to an unusual polyamine , also PKS - derived . - pubmed
In this study , we hypothesized that heme oxygenase-1 ( HO-1 ) , an enzyme responsible for degradation of heme to carbon monoxide ( CO ) , bilirubin , and iron is an important regulator of inflammation and epithelial responses in the prostate . - pubmed
Conversely , exogenous CO ( 250 ppm ) increased IL-1beta levels and suppressed cell proliferation in the prostates . - pubmed
Interestingly , immunomodulatory effects of HO-1 and CO correlated with early induction of the long - chain acyl - CoA synthetase 1 ( ACSL1 ) . - pubmed
Therefore , we investigated the electrophysiological changes and the concomitant mechanical responses according to the expression levels of the KCNJ2 E299V mutation during sinus rhythm and ventricular fibrillation . - pubmed
We concluded that in both sinus rhythm and fibrillation , the KCNJ2 E299V mutation results in very low contractility regardless of the expression level of mutation and increases the risk of cardiac arrest and cardiac death . - pubmed
Role of Chrysophanol in Epithelial - Mesenchymal Transition in Oral Cancer Cell Lines via a Wnt-3 - Dependent Pathway . - pubmed
We further speculated that chrysophanol - mediated EMT and metastasis are involved in the Wnt-3 - dependent signaling pathway . - pubmed
The inhibition of the EMT phenotype and metastasis and accumulation of ROS caused by chrysophanol was reversed by treatment with the Wnt-3 agonist Bml 284 . - pubmed
Therefore , our findings indicated that chrysophanol altered EMT formation , ROS accumulation , and metastasis via the Wnt-3 - dependent signaling pathway . - pubmed
The endogenous production of H2S is primarily mediated by cystathione beta - synthase ( CBS ) , cystathione gamma - lyase ( CSE ) , and 3 - mercaptopyruvate sulfurtransferase ( 3 - MST ) . - pubmed
Finally , we show that identical neuropeptides were detected between tissues treated with 10 % NBF and an aqueous 1 % w / v sodium phosphate solution ( composition of 10 % NBF without formaldehyde ) , suggesting that utilizing a salt solution wash affects neuropeptide detection and formaldehyde does not affect neuropeptide detection when our wash protocol is performed . - pubmed
Also , the serum level of interleukin-10 was increased after 3 days of VitD supplementation ( p = 0.001 ) . - pubmed
Baseline integrase drug resistance mutations and conserved regions across HIV-1 clades in Cameroon : implications for transition to dolutegravir in resource - limited settings . - pubmed
BACKGROUND : Transition to dolutegravir - based regimens in resource - limited settings ( RLS ) requires prior understanding of HIV-1 integrase variants and conserved regions . - pubmed
Of 288 HIV-1 integrase amino acid positions , 58.3 % were highly conserved across subtypes in the following major regions : V75 - G82 , E85 - P90 , H114 - G118 , K127 - W132 , E138 - G149 , Q168 - L172 , T174 - V180 , W235 - A239 and L241 - D253 . - pubmed
Melatonin can be measured in different body fluids , such as CSF , blood , saliva and urine . - pubmed
Frozen ground muscle , when compared to unfrozen ground tissue , showed significantly lower hexose monophosphates and significantly greater levels of all metabolites from fructose diphosphate to phosphoenolpyruvate , indicating activation of phosphofructokinase and inhibition of pyruvate kinase during the freezing process . - pubmed
Thawing caused rapid glycolysis and the metabolites returned to near unfrozen levels by the time glycolysis ceased , due to strong activation of phosphorylase by Ca2 + . - pubmed
After sucrose gradient centrifugation , the densest subfraction ( sarcoplasmic reticulum ) contained the highest ( K + , Ca2 +)- ATPase activity and virtually no ( Na2 + , K +)- ATPase activity , even when latent ( Na + , K +)- ATPase activity was unmasked . - pubmed
In addition , the sarcoplasmic reticulum fraction contained no significant sialic acid , beta receptor binding activity , or adenylate cyclase activity . - pubmed
The results suggest that ( Na + , K +)- ATPase , sialic acid , beta - adrenergic receptors , and adenylate cyclase can be entirely accounted for by the sarcolemmal content of cardiac microsomes . - pubmed
Cyclic AMP stimulated phosphorylation by [ gamma-32P ] ATP of two proteins of apparent Mr = 20,000 and 7,000 that were concentrated in sarcoplasmic reticulum , but the stimulation was markedly dependent on the presence of added soluble cyclic AMP - dependent protein kinase . - pubmed
Cyclic AMP also stimulated phosphorylation of membrane proteins in sarcolemma , but this phosphorylation was mediated by an endogenous protein kinase activity . - pubmed
In cirrhotics and in healthy controls the t 1/2 Leu was shortened during the additional glucose administration compared with the t 1/2 Leu after leucine administration alone and was prolonged by inhibiting insulin secretion by somatostatin . - pubmed
Furthermore , subjects treated with an ACE - inhibitor ( enalapril ) showed significant reduction in acetylcholine - evoked axon reflex responses , while the test group of smokers showed a significant reduction in their dilator response to pilocarpine.(ABSTRACT TRUNCATED AT 400 WORDS ) - pubmed
Normovolemic hemodilution under normoxic conditions ( arterial oxygen partial pressure normal ) at rest is limited by the critical arterial oxygen content of about 10 ml / dl corresponding to a hemoglobin concentration of 7.5 g / dl . - pubmed
Furthermore , stored blood ( blood bag ) seems to be of minor therapeutic effect because of the left shifted oxygen content curve combined with a reduced effective Hb concentration . - pubmed
Tout2 but not Tout1 was blocked by intracellular injection of 300 - 600 pmol , BaCl2 ( final concentration in the oocyte : 0.3 - 0.6 mM ) . - pubmed
Injection of KCl had no effect on either Tout1 or Tout2 . - pubmed
Tout2 but not Tout1 was enhanced by low concentrations of serotonin ( 0.5 - 2 nM ) . - pubmed
Higher concentrations ( above 10 nM ) of serotonin decreased the amplitude of Tout2 . - pubmed
The effect of serotonin was blocked by the protein kinase inhibitor , H-7 ( 25 microM ) . - pubmed
Tout2 but not Tout1 was enhanced by 10 nM - phorbol myristate acetate . - pubmed
Higher concentrations of the phorbol ester decreased the amplitude of Tout2 . - pubmed
This current reflects a second process of chloride channel opening which can be enhanced by serotonin via activation of protein kinase C. - pubmed
Influence of monosaccharides on the fibrillogenesis of type I collagen . - pubmed
The in vitro self - assembly of Type I rat tail tendon collagen in the presence of monosaccharides was studied turbidimetrically . - pubmed
The molecular mass of this membrane - bound adrenal trypsin - like enzyme ( mATLE ) is 31 kDa as determined by sodium dodecyl sulfate - polyacrylamide gel electrophoresis , and the enzyme is extremely basic , binding to carboxymethyl - Sephadex at pH 8.5 . - pubmed
The pH optimum of mATLE using t - butoxycarbonyl - Glu - Lys - Lys - aminomethylcoumarin as a substrate is 8.5 - 8.7 , and its Km value for this substrate is 2.2 mM. - pubmed
Sequencing of cleavage products released from Peptide B revealed that the enzyme preferentially cleaves between and following the paired basic residues at positions 23 and 24 of Peptide B ( thus generating [ Met - enkephalin ]- Arg - Phe and Arg -[ Met - enkephalin ]- Arg - Phe ) . - pubmed
The phenotype of the common , immature thymocyte was identified by the binding of either fluorescein isothiocyanate ( FITC)- or phycoerythrin ( PE)-conjugated monoclonal anti - T6 ( OKT 6 ) antibody and of mature thymocytes by the binding of monoclonal anti - T3 ( OKT 3 ) antibody . - pubmed
Plasma total , high - density - lipoprotein ( HDL ) , low - density - lipoprotein and very - low - density - lipoprotein cholesterol and triglycerides were determined weekly . - pubmed
A significant effect ( P less than 0.05 ) of dietary fat on plasma total and HDL cholesterol was observed between the end of wk 2 and 7 . - pubmed
Low fat intake resulted in 17 + /- 2 mg / dL lower total cholesterol and 10 + /- 1 mg / dL lower HDL cholesterol than the usual fat intake . - pubmed
Intracellular calcium release mediated by two muscarinic receptor subtypes . - pubmed
By combining fluorescent indicator dye ( fura-2 ) studies with electrophysiological measurements it is shown that stimulation of mAChR I and mAChR III readily leads to release of calcium from intracellular stores and to associated conductance changes , whereas stimulation of mAChR II and mAChR IV exerts no such effect . - pubmed
Dose - response curves describing the amplitude or the delay of the calcium rise induced by acetylcholine suggest that the apparent affinity of mAChR III for its agonist is higher by about one order of magnitude than that of mAChR I. - pubmed
Thiol / disulfide exchange between 3 - hydroxy-3 - methylglutaryl - CoA reductase and glutathione . - pubmed
At pH 7.0 , 37 degrees C , the second order rate constant for inactivation of the reduced enzyme by GSSG is 1700 + /- 200 M-1 min-1 , approximately 20 - fold faster than the reaction of GSSG with a typical , unhindered thiol of pKa 7.7 . - pubmed
High concentrations of GSH or lower concentrations of dithiothreitol restore the activity of the oxidized enzyme . - pubmed
The oxidation of the enzyme by GSSG is only 30 - fold slower in the presence of saturating levels of both substrates . - pubmed
The incomplete inhibition of thiol / disulfide exchange by substrates can lead to significant changes in the activity of the enzyme during the assay when glutathione is present . - pubmed
At redox equilibrium , both in the absence and presence of substrates , the activity of the enzyme depends on the quantity [ GSH]2/[GSSG ] , suggesting that the redox transition involves the formation of a protein - SS - protein disulfide . - pubmed
Thus , HMG - CoA reductase is very sensitive to dithiol oxidation both kinetically and thermodynamically . - pubmed
Significant changes in the oxidation state and activity of this enzyme could be expected to result from normal changes in the thiol / disulfide oxidation state of the cellular glutathione redox buffer . - pubmed
Role of iodinated hormones , parathyroid hormone , calcitonin , vitamin D and blood calcium levels ] . - pubmed
The decay rate of the excited triplet state of Zn cytochrome c was enhanced by electron acceptors including methyl viologen and ferric complexes of cyanide , oxalate , EDTA and cytochrome c at room temperature . - pubmed
After incubation of the cells to 1 microM ( 3H ) 7 - OH - MTX , four radiolabelled peaks , representing 75 % of the intracellular 3H , were converted to 7 - OH - MTX upon exposure to hog kidney conjugase indicating the formation of 7 - OH - MTX polyglutamyl derivatives . - pubmed
Change and correlates of change in high and low density lipoprotein cholesterol after six years : a prospective study . - pubmed
Change and correlates of change in high and low density lipoprotein cholesterol ( HDL and LDL ) were studied in a prospective population - based study of 614 men and women in Rancho Bernardo , CA , 1972 - 1980 . - pubmed
Among the behavioral variables , HDL change was positively associated with change in alcohol use in both sexes and with change in postmenopausal estrogen use in women and was inversely associated with change in obesity index in men . - pubmed
Effect of pretreatment of animals with ethidium bromide , an intercalating dye which has been shown to prevent the in vitro nuclear binding of steroid hormone - receptor complexes , on the depletion of receptor induced by testosterone administration was also insignificant . - pubmed
This drug does not stimulate insulin release but works by lowering glucose in peripheral tissues . - pubmed
Interferon alpha induces rapid tyrosine phosphorylation of the vav proto - oncogene product in hematopoietic cells . - pubmed
The IFN alpha - induced tyrosine phosphorylation of p95vav was time- and dose - dependent , confirming the specificity of the process . - pubmed
After IFN alpha stimulation significant amounts of phosphorylation of p95vav on tyrosine residues were detectable . - pubmed
Monoaldehyde allosteric effectors of hemoglobin were designed , using molecular modeling software ( GRID ) , to form a Schiff base adduct with the Val 1 alpha N - terminal nitrogens and interact via a salt bridge with Arg 141 alpha of the opposite subunit . - pubmed
It was envisioned that the molecules , which are aldehyde acids , would produce a high - affinity hemoglobin with potential interest as antisickling agents similar to other aldehyde acids reported earlier . - pubmed
However , oxygen equilibrium curves run on solutions obtained from T- ( tense ) state hemoglobin crystals of reacted effector molecules produced low - affinity hemoglobins . - pubmed
The GABA - A agonist muscimol , but not MK-801 , was effective if given 30 , 45 , or 60 min after embolization ( potency ratio compared with saline of 3.0 , 2.3 , 1.8 , respectively ) . - pubmed
Agonists of GABA - A , but not GABA - B , receptors blocked the toxic vacuolization seen in the cingulate and retrosplenial cortex after MK-801 treatment . - pubmed
Both sets of phosphopeptides contained candidate MAPK target sites at Thr292 and Thr386 that fit the consensus sequence ProXThr*Pro . - pubmed
Replacement of either Thr292 or Thr386 with alanine by site - directed mutagenesis reduced the phosphate incorporation respectively to 32 or 75 % that of wild type MKK . - pubmed
We conclude that the phosphorylation of MKK at Thr292 and Thr386 does not interfere with catalysis in vitro . - pubmed
Other receptors that stimulate APPs secretion include those for bradykinin , vasopressin , and interleukin-1 receptors . - pubmed
This increase is tetrodotoxin - sensitive and frequency - dependent , suggesting that APPs release may normally depend on neuronal activity . - pubmed
For 2 - , 3 - , 4 - , and 6 - fluorodeoxyglucose analogs , the alpha anomers permeate more rapidly , and the P alpha / P beta ratio is dependent on the position of fluorination , with values of 1.1 , 1.3 , 2.5 , and 1.6 , respectively , obtained at 37 degrees C. These results have been analyzed in terms of a simple alternating conformation model for the glucose transporter . - pubmed
Progesterone has been implicated to play a critical role in mediating LH induction of ovulation and possibly luteinization . - pubmed
Transcripts of approximately 11.0 , 7.2 , 6.8 , 6.2 , 3.4 , and 3.1 kilobases in size were induced by ovulatory ( 500 ng / ml ) , but not low ( 50 ng / ml ) , concentrations of LH and FSH as well as by forskolin ( 10 microM ) , GnRH ( 1 microM ) , and phorbol 12 - myristate 13 - acetate ( 200 nM ) . - pubmed
The antiprogestins RU486 and ZK98299 did not inhibit induction of PR mRNA and protein , but effectively blocked agonist activation of glucocorticoid response element2 - E1b - chloramphenicol acetyltransferase , as well as LH stimulation of luteinization in vitro . - pubmed
These results provide direct evidence that agonists stimulating diverse intracellular pathways can induce PR in granulosa cells , that progesterone plays a functional role in the luteinization process triggered by the LH surge , and that the effects are mediated at least in part by induction of PR . - pubmed
Unlike other fructose using tissues , where Glut-5 may subserve the role of a fructose carrier , in brain where fructose is not used as a substrate , Glut-5 may transport glucose alone . - pubmed
All the doses of Amitriptyline produced significant hyperglycemia at 4 h , which attained a peak at 24 h with 16 mg / kg dose and appears to be due to blockade of the uptake of monominergic transmitters across the axoplasmic membrane , It ( 16 mg / kg ) also produced glucose intolerance during early hours probably due to interference with gastrin function . - pubmed
The amino acid sequence of the deduced peptide showed a typical flavin adenine dinucleotide - binding site in its N - terminal region , confirming the flavoproteinic structure of delta 4 ( 5 alpha ) DH . - pubmed
The involvement of catecholamines , in hypertension , alcohol and ACE - inhibition . - pubmed
We have attempted to provide a progress report on current research on the role of catecholamines and serotonin receptor subtypes in feeding control . - pubmed
Chick cDNAs identified included : the parathyroid hormone - related protein , an immediate early gene ; the delta - subunit of the neuronal - specific Ca2 +/ calmodulin - regulated protein kinase II ; and the GTP - binding protein CDC42 , a member of the ras superfamily of G proteins . - pubmed
These results indicate the potential involvement of both Ca2 +/calmodulin - mediated signaling and GTPase cascades in the response to noise damage and during hair cell regeneration in the chick basilar papilla . - pubmed
It is metabolized by the cytochrome P-450 system to a stable , pharmacologically active epoxide intermediate , carbamazepine-10 , 11 - epoxide . - pubmed
Comparative studies of the phage T2 and T4 DNA ( N6 - adenine ) methyltransferases : amino acid changes that affect catalytic activity . - pubmed
Kinetic analyses with purified homogeneous enzymes showed that the two MTases had similar Km values for the methyl donor , S - adenosyl - L - methionine , and for substrate DNA . - pubmed
Since the T2 and T4 MTases differ at only three amino acid residues ( at positions 20 [ T4 , Ser ; T2 , Pro ] , 26 [ T4 , Asn ; T2 , Asp ] , and 188 [ T4 , Asp ; T2 , Glu ] ) , we have produced hybrid proteins to determine which residue(s ) is responsible for increased catalytic activity . - pubmed
BACKGROUND : A synthetic peptide corresponding to residues 75 - 84 of the alpha1 domain of HLA - B7 molecule ( HLA - B7.75 - 84 [ Allotrap ] ) inhibits cytotoxic T cell function in vitro and , when combined with subtherapeutic doses of cyclosporine ( CsA ) , prolongs allogeneic cardiac survival . - pubmed
alone or with CsA plus alternate - day HLA - B7.75 - 84 ( 20 mg / kg / day i.p . ) - pubmed
RESULTS : HLA - B7.75 - 84 plus CsA prolonged allograft survival ( 75 % of allografts survived & gt;90 days ) compared with CsA alone ( 27 % of allografts survived & gt;90 days ) ( P<0.05 ) . - pubmed
Results suggest that the estrus - inducing action of LHRH , PGE2 and db cAMP occurs through the activation of the progesterone receptor . - pubmed
Proton release upon glutathione binding to glutathione transferase P1 - 1 : kinetic analysis of a multistep glutathione binding process . - pubmed
The fate of the thiol proton coming from the ionization of the sulfhydryl group of GSH in the active site of glutathione transferase P1 - 1 has been studied . - pubmed
pH changes caused by the binding of GSH to the enzyme in the absence of any inorganic buffer indicate that the thiol proton leaves the active site when the binary complex is formed . - pubmed
By comparing the kinetic behavior of three distinct events occurring during the binding of GSH to the enzyme , i. e. , proton release , ionization of bound GSH and quenching of intrinsic fluorescence , it appears that the binding process follows a multistep mechanism possibly involving the conformational transition of a weak precomplex into the final Michaelis complex . - pubmed
Immunohistochemical analysis with monoclonal antibodies to bcl-2 oncoprotein in formalin - fixed paraffin - embedded tissue sections revealed that severe epithelial dysplasias had a higher percentage of immunoreactivity than did mild and moderate dysplasias and squamous cell carcinomas . - pubmed
Interactions with the promoter for the flagellin gene fliC were analyzed using DNase I and KMnO4 footprinting over a range of temperatures . - pubmed
In contrast to the previously published hMUC3 sequence , which terminates shortly after a single cysteine - rich EGF - like domain , conceptual protein translation of the dominant and largest PCR product identified two extracellular cysteine - rich EGF - like domains separated by an N - glycosylation - rich domain and a potential coiled - coil region , followed by a putative transmembrane region and a 75 amino acid cytoplasmic tail . - pubmed
Roles of his205 , his296 , his303 and Asp259 in catalysis by NAD +- specific D - lactate dehydrogenase . - pubmed
The role of three histidine residues ( His205 , His296 and His303 ) and Asp259 , important for the catalysis of NAD +- specific D - lactate dehydrogenase , was investigated using site - directed mutagenesis . - pubmed
None of these residues is presumed to be involved in coenzyme binding because Km for NADH remained essentially unchanged for all the mutant enzymes . - pubmed
These analyses revealed that the gene encoding Dab2 , a mitogen - responsive phosphoprotein , is differentially expressed in wild - type and GATA-6 - deficient ES cells . - pubmed
Taken together , these data demonstrate that the mitogen - responsive phosphoprotein Dab2 is a downstream target of GATA-6 in the visceral endoderm . - pubmed
Characterization of the new alpha-1 - antitrypsin - deficient PI M - type allele , PI M(vall d'hebron ) ( Pro(369)-->Ser ) . - pubmed
RESULTS : Regarding acute phase response , the EAAA and OAAA groups showed significant increases in C - reactive protein ( P & lt;.001 and P = .001 ) , body temperature ( P = .035 and P = .048 ) , and leukocyte count ( P & lt;.001 and P & lt;.001 ) . - pubmed
Regarding complement activation , the C3d / C3 ratio disclosed significant postoperative elevations in the EAAA and OAAA groups ( P = .013 and P = .009 ) . - pubmed
Meanwhile , ATP - induced DNA fragmentation was fully blocked by pretreatment with oxidized ATP ( OxATP ) , a compound recently shown to block P2z receptors . - pubmed
CONCLUSION : These data indicate that P2z receptors on lymphocytes play an important role in apoptosis induced by ATP in vitro . - pubmed
The protein is examined in powder form , in D ( 2 ) O , and in four two - component perdeuterated single - phase cryosolvents in which it is active and stable . - pubmed
The dynamic transition in all of the mixed cryosolvent - protein systems is much more gradual than in pure D ( 2 ) O , consistent with a distribution of energy barriers . - pubmed
GM - CSF regulates bleomycin - induced pulmonary fibrosis via a prostaglandin - dependent mechanism . - pubmed
Exogenous addition of GM - CSF to alveolar macrophages reversed the PGE2 synthesis defect in vitro . - pubmed
The enzymes involved in the production of the precursors of carbamyl phosphate and nitrogen assimilation , glutamine synthetase and nitrate reductase , were also investigated , and it appears that these two enzymes are present in the bacteria . - pubmed
The radiolabeled DAT ligand [ 3H ] GBR12935 was bound to peripheral lymphocytes in a manner consistent with the specific binding to a dopamine uptake system , with a dissociation constant similar to that found in striatum , but with a lower density of binding sites . - pubmed
Western blot analysis using antibodies raised against amino or carboxy terminus of DAT or against VMAT-1 or VMAT-2 revealed labeling of single bands of approximately 76 , 55 or 68 KDa , respectively , displaying similar migration characteristics in lymphocytes and test tissues used for comparison . - pubmed
Immunofluorescence revealed that anti - dopamine , anti - tyrosine hydroxylase , anti - DAT , anti - VMAT-1 and anti - VMAT-2 antibodies labeled the total population of cytospin - centrifuged lymphocytes mounted on microscope slides . - pubmed
Confocal laser microscopy demonstrated that dopamine and VMAT-2 immunoreactivity was developed mainly in cytoplasmic punctiform areas likely corresponding to vesicles and to a lower extent was associated to plasma membrane . - pubmed
Lymphocyte DAT characterized in this study has probably functional relevance as [ 3H ] dopamine was taken up by intact lymphocytes and uptake was inhibited specifically by compounds known to affect dopamine transport . - pubmed
Although this therapy has been traditionally performed by oral estrogen or bilateral orchiectomy , there are now two most important pharmacological hormonal therapies : LH - RH agonist and antiandrogen therapy . - pubmed
Pain associated with injection of botulinum A exotoxin reconstituted using isotonic sodium chloride with and without preservative : a double - blind , randomized controlled trial . - pubmed
Wortmannin , a specific inhibitor of phosphatidylinositol 3 - kinase ( PI3 K ) , mimicked the priming irradiation in that the inhibitor alone rendered the cells resistant against the induction of chromosome aberrations and apoptosis by the subsequent X - ray irradiation . - pubmed
Sonochemiluminescence ( SCL ) of luminol due to a single bubble is studied through spectral measurement . - pubmed
The first receptor model is a self training factor analysis ( FA ) model with non - negative constraints that was applied to identify the PCB sources and significant congener patterns . - pubmed
The FA model indicated two significant factors , the major one being Aroclor 1242 and the other , a profile dominated by low chlorinated congeners , indicating a possible PCB alteration profile . - pubmed
It was also deduced from the FA model that very small contributions of more highly chlorinated Aroclors may be present in the system . - pubmed
Here we apply extended X - ray absorption fine structure ( EXAFS ) measurements to characterize the metal ion binding site , in frozen solution , of the unimolecular quadruplex formed by the thrombin binding aptamer , d(G(2)T(2)G(2)TGTG(2)T(2)G(2 ) ) ( TBA ) , in the presence of Pb ( 2 + ) ions . - pubmed
The Pb L ( III ) -edge X - ray absorption spectrum of this metal - DNA complex is very similar to that we obtain for a Pb ( 2 +)-stabilized quartet system of known structure constructed from a modified guanine nucleoside ( G1 ) . - pubmed
The Fourier transforms of the Pb ( 2 + ) complexes with both TBA and G1 show a first - shell interaction at about 2.6 A , and a weaker , broader shell at 3.5 - 4.0 A. Quantitative analysis of the EXAFS data reveals the following : ( i ) very close agreement between interatomic distances at the metal coordination site for the Pb ( 2 +)- G1 complex determined by EXAFS and by X - ray crystallography ; ( ii ) similarly close agreement between interatomic distances measured by EXAFS for the Pb ( 2 +)- G1 and Pb ( 2 +)- TBA complexes . - pubmed
These results provide strong evidence for binding of the Pb ( 2 + ) ion in the region between the two quartets in the Pb ( 2 +)- TBA complex , coordinated to the eight surrounding guanine O6 atoms . - pubmed
RESULTS : Cumulative oxidation of ( 13 ) C - oleate at 11.5 h postdose was significantly greater during the heavy exercise ( 49 + /- 4 % ) trial than both the light exercise ( 39 + /- 4 % ) and the rest trials ( 34 + /- 4 % ) ( P & lt ; 0.005 ) . - pubmed
Oxidation of d ( 31 )- palmitate recovery at 11.5 h postdose was not different during the heavy exercise ( 12 + /- 1 % ) , light exercise ( 11 + /- 1 % ) , and rest trials ( 10 + /- 1 % ) . - pubmed
Culture of G(0 ) CD34(+ ) cells isolated based on Hoechst 33342 / PyroninY staining with Tpo , SCF and FL for 48 hrs , results in significantly elevated Survivin mRNA and protein levels . - pubmed
Moreover , labeling of fresh G(0 ) CD34(+ ) cells with 5 -( and 6 - ) carboxyfluorescein diacetate succinimidyl ester ( CFSE ) before culture with growth factors for up to 72 hrs , revealed that Survivin expression was elevated in CFSE(bright ) G(0 ) CD34(+ ) cells , indicating that up - regulation occurred before entry into G1 . - pubmed
In addition , G - Rh ( 2 ) almost completely inhibited telomerase activity , as measured by polymerase chain reaction - based telomeric repeat amplification protocol coupled with enzyme - linked immune sorbent assay , and human telomerase reverse transcriptase mRNA . - pubmed
MATERIAL / METHODS : Postmortem infarct volume was determined by quantitative image analysis of triphenyltetrazolium - stained brain sections , collected at 1 h , 3 h and 24 h after initiation of cerebral ischemia , to measure consistent infarct volumes of the post -ischemic gerbil brain . - pubmed
Acute administration of clonidine ( 2 mg / kg ) , an alpha2AR agonist , elicited dramatic decreases in DNA synthesis in the forebrain , brainstem , and cerebellum whether given on gestational day ( GD ) 21 , or on postnatal days ( PN ) 1 or 8 . - pubmed
However , alpha2AR blockade elicited by yohimbine ( 2.5 mg / kg ) also resulted in decreased DNA synthesis on GD21 and PN8 , albeit to a smaller extent than with clonidine . - pubmed
Because betaARs are also known to regulate DNA synthesis , we used propranolol ( 10 mg / kg ) blockade of betaARs to evaluate whether the alpha2AR effects were mediated by presynaptic autoreceptors that regulate the release of norepinephrine and consequent betaAR responses ; the effects of yohimbine were still discernible in the presence of propranolol . - pubmed
Although the role of PTP-1B in dephosphorylating various cell surface receptor tyrosine kinases is clear , the mechanisms by which it modulates receptor function from the endoplasmic reticulum ( ER ) remains an enigma . - pubmed
AG490 , a tyrosine kinase inhibitor affecting Jak proteins , inhibits CD2 - mediated IFN - gamma mRNA expression , secretion , and nucleoprotein binding to the IFN - gamma STAT5 site in a dose - dependent fashion . - pubmed
Dodecanethiol MPNs , in which the monolayer is dodecanethiol linked to the gold nanoparticle , have shown great promise as a GC stationary phase with efficient columns having been produced in a variety of capillary i.d . - pubmed
The efficiency of the dodecanethiol MPN stationary phase was determined with a 100 microm i.d . - pubmed
Areas of application illustrated and discussed utilizing the dodecanethiol MPN stationary phase include complementary separations such as two - dimensional GC ( GC x GC ) , potential utilization within a model system for a micro - fabricated GC ( microGC ) , as well as efficient single dimension high - speed separations . - pubmed
Included is a selectivity comparison of the retention behavior of the 4 - chlorobenzenethiol MPN stationary phase and the dodecanethiol MPN stationary phase . - pubmed
The anti - albuterol serum was treated with saturated ammonium sulfate solution to obtain the antibody ( immune globulin ) . - pubmed
The results showed that the immune globulin - affinity column has the characteristic of high purity and high specificity , and it is suitable for the preparation of chromatographic plasma specimen to determine albuterol in plasma . - pubmed
PURPOSE : Fructose 2,6 - bisphosphate ( F2,6BP ) is a potent activator of phosphofructokinase , which is a rate - limiting enzyme of glycolysis . - pubmed
The concentration of F2,6BP depends on the activity of the bifunctional enzyme , 6 - phosphofructo-2 - kinase / fructose-2,6 - bisphosphatase ( PFK-2 / FBPase ) . - pubmed
Quantitative , real - time PCR analysis confirmed the overexpression of PFKFB3 mRNA in colon carcinoma , with the dominant variant being the PFKFB3 - ACG isoform that contains a phosphorylation site at Ser461 . - pubmed
Roles of the threonine 407 , aspartic acid 417 , and threonine 419 residues in P450 2B1 in metabolism . - pubmed
We have previously observed that the quadruple ( S407T - N417D - A419T - K473 M ) and triple ( S407T - N17D - A419 T ) mutants of the chimeric construct of P450 2B1 / 2B2 do not undergo mechanism - based inactivation by 17alpha - ethynylestradiol ( 17EE ) and tert - butyl 1 - methyl-2 - propynyl ether ( tBMP ) . - pubmed
In the presence of the alternate oxidants cumene hydroperoxide and tert - butyl hydroperoxide , the wild - type 2B1 was not inactivated by 17EE . - pubmed
Metabolism of 17EE by 2B1 supported by these alternate oxidants revealed differences in the metabolites that may be related to the inability of 2B1 to be inactivated under these conditions . - pubmed
Apoptosis was detected by means of annexin V and propidium iodide staining . - pubmed
SCF synergized with chemoattractants in causing basophil upregulation of the integrin CD11b , and this effect was inhibited by a c - kit antibody , the tyrosine kinase inhibitor imatinib mesylate ( STI-571 ) , and a phosphatidylinositol 3 kinase inhibitor but not by inhibitors of p38 mitogen - activated protein kinase or mitogen - activated protein kinase / extracellular signal - regulated kinase kinase . - pubmed
Thiazolo [ 5,4 - f ] quinazolin-9 - ones , inhibitors of glycogen synthase kinase-3 . - pubmed
In an effort to identify new protein kinase inhibitors with increased potency and selectivity , we have developed the microwave - assisted synthesis of thiazolo [ 5,4 - f ] quinazolin-9 - ones . - pubmed
Molecular modeling studies suggest that the most selective GSK-3 inhibitors 7a - d bind into the ATP - binding site through a key hydrogen bond interaction with Val135 and target the specific hydrophobic backpocket of the enzyme . - pubmed
The thermal behavior of polyester - polyurethane and polyether - polyurethane in air atmosphere was investigated by using FTIR analysis in the temperature range from 20 to 400 degrees C. The thermal degradation behaviors of polyester - polyurethane and polyether - polyurethane in different atmospheres were investigated by using thermogravimetry analysis ( TG ) in the temperature range from 20 to 700 degrees C. The FTIR results together with the TG investigations showed that polyester - polyurethane has two degradation steps in air , but polyether - polyurethane has only one degradation step . - pubmed
It is therefore assumed that the supraordinated hormones , GnRH , FSH , and/or LH , have an effect on the urodynamic parameters . - pubmed
Within 1 week of GnRH analogue administration , plasma FSH and LH levels decreased from 72.5 and 7.7 to 7.75 and 0.72ng / mL , respectively . - pubmed
The improvement in bladder function after the application of GnRH - application is presumably a direct effect of the GnRH as a relationship between the plasma gonadotropin levels and the urodynamic parameters could not demonstrated . - pubmed
Aggregated silver nanoplates can serve as active SERS substrates with an enhancement factor of about 4.5 x 10(5 ) using 2 - aminothiophenol as a probing molecule . - pubmed
RESULTS : The seropositive rates for HBsAg , anti - HBs , and anti - HBc were 1.2 % , 50.5 % , and 3.7 % , respectively , in those born after the vaccination program ( & lt;20 years of age ) in 2004 . - pubmed
polygyrus were resistant to trinitrobenzenesulfonic acid ( TNBS)-induced colitis , a Th1 cytokine - dependent inflammation . - pubmed
Furthermore , an association study was performed , testing three single nucleotide polymorphisms ( SNPs ) of suppressor of cytokine signalling 3 ( SOCS3 ) in 162 NR and 184 SVR subjects ( SOCS3 -8464 A / C ( rs12952093 ) , -4874 A / G ( rs4969170 ) and 1383 A / G , ( rs4969168 ) ) . - pubmed
Of critical importance to sustainable agriculture are the symbioses formed between more than 80 % of terrestrial plants and arbuscular mycorrhizal ( AM ) fungi and between legumes and nitrogen - fixing rhizobial bacteria . - pubmed
MtDMI3 encodes a Ca ( 2 +)/ calmodulin - dependent protein kinase ( CCaMK ) essential for the transduction of the Ca ( 2 + ) signal induced by the perception of Nod factors . - pubmed
Use of aromatase inhibitors rather than tamoxifen for breast cancer will likely lead to more osteoporosis . - pubmed
STM results indicated that the symmetry of ordered structures decreased as the alkyl chain became longer , with the exception of C ( 6 ) OPc , which preferentially formed a quasi-3 - fold symmetrical structure . - pubmed
We studied the potential of systemically administered aminoglycosides as a therapy for retinal degeneration resulting from premature termination codon ( PTC ) mutations . - pubmed
These experiments indicated that gentamicin treatment induced on average a 5.3 % extra read - through of the S334ter PTC in vitro , but did not affect the rd12 PTC . - pubmed
Two different chitosan ( CS ) nanocarriers namely nanoparticles and nanoemulsion were developed to prolong Indomethacin ( IM ) precorneal residence time and to improve its ocular bioavailability the main limitations in its management of post - operative inflammation and intraocular irritation after cataract extraction . - pubmed
CS - nanoparticles were developed by modified ionic gelation of CS with tripolyphosphate while nanoemulsion was prepared by spontaneous emulsification technique . - pubmed
H2O2 - dependent hyperoxidation of peroxiredoxin 6 ( Prdx6 ) plays a role in cellular toxicity via up - regulation of iPLA2 activity . - pubmed
Peroxiredoxin 6 ( Prdx6 ) is a bifunctional enzyme with peroxidase activity and Ca2 + -independent phospholipase A2 ( iPLA2 ) activity . - pubmed
Here , we report that H2O2 - induced cellular toxicity acts through Prdx6 hyperoxidation . - pubmed
Under high concentrations of H2O2 ( & gt ; 100 microm ) , Prdx6 , and 2 - Cys Prdxs were hyperoxidized . - pubmed
Contrary to hyperoxidation of 2 - Cys Prdxs , hyperoxidation of Prdx6 was irreversible in vivo . - pubmed
Surprisingly , H2O2 - induced cell cycle arrest at the G2 / M transition correlated with hyperoxidation and increased iPLA2 activity of Prdx6 . - pubmed
Furthermore , the H2O2 - mediated increase in iPLA2 activity was dramatically abolished in a hyperoxidation mutant ( C47A ) , an iPLA2 mutant ( S32A ) , and a double mutant ( C47A / S32A ) of Prdx6 , demonstrating the essential requirement of Prdx6 C47 hyperoxidation for its iPLA2 activity . - pubmed
Together , our results demonstrate that H2O2 - mediated hyperoxidation of Prdx6 induces cell cycle arrest at the G2 / M transition through up - regulation of iPLA2 activity . - pubmed
Desflurane but not sevoflurane can increase lung resistance via tachykinin pathways . - pubmed
Antagonization of tachykinin receptors of airway smooth muscles completely diminished the increase in R(L ) induced by desflurane . - pubmed
Here , we show that exposing DT104 to mitomycin C , a DNA - damaging agent , induced production of prophage - encoded ArtA / ArtB. - pubmed
Pertussis - sensitive G proteins were labelled in the presence of [ ( 32 ) P ] NAD and ArtA , and the label was released by HgCl ( 2 ) , which is known to cleave cysteine - ADP - ribose bonds . - pubmed
ADP - dependent modification of G proteins was markedly reduced in in vitro - synthesized ArtA(6Arg - Ala ) and ArtA(115Glu - Ala ) , in which alanine was substituted for the conserved arginine at position 6 ( necessary for NAD binding ) and the predicted catalytic glutamate at position 115 , respectively . - pubmed
Hydrogen peroxide , an oxidative stressor , also induced ArtA / ArtB production , suggesting that these agents induce in vivo synthesis of ArtA / ArtB. - pubmed
These results , taken together , suggest that ArtA / ArtB is an active toxin similar to PTX . - pubmed
Tissue inhibitor of matrix metalloproteinase-1 reduces phosphatidylserine exposure on activated and aged platelets . - pubmed
The modulation transfer function ( MTF ) of the system was determined from simulated images of a point source of ( 99m ) Tc , positioned at different depths in a water phantom . - pubmed
Electrophysiological data have shown that the acidic residues E106 in transmembrane helix 1 ( TM1 ) and E190 in TM3 contribute to the high selectivity of ORAI1 channels for Ca ( 2 + ) . - pubmed
We have examined the pore architecture of the ORAI1 channel using ORAI1 proteins engineered to contain either one or two cysteine residues . - pubmed
Disulfide cross - linking shows that ORAI1 assembles as a tetramer or a higher oligomer with TM1 centrally located . - pubmed
Cysteine side chains projecting from TM1 at position 88 , 95 , 102 , or 106 cross - link efficiently to the corresponding side chain in a second ORAI1 monomer . - pubmed
Cysteine residues at position 190 or at surrounding positions in TM3 do not cross - link . - pubmed
We conclude that E106 residues in wild - type ORAI1 are positioned to form a Ca ( 2 + ) binding site in the channel pore and that E190 interacts less directly with ions traversing the pore . - pubmed
Later on , after World War II and the abolition of the camphor monopoly , the company started manufacturing products related to oils and fats , including higher fatty acids , and expanded its business by developing a new field of chemical industry . - pubmed
Characterization of in vivo keratin 19 phosphorylation on tyrosine-391 . - pubmed
Although K19 is known to be phosphorylated on tyrosine residue(s ) , conclusive site - specific characterization of these residue(s ) and identification potential kinases that may be involved has not been reported . - pubmed
METHODOLOGY / PRINCIPAL FINDINGS : In this study , biochemical , molecular and immunological approaches were undertaken in order to identify and characterize K19 tyrosine phosphorylation . - pubmed
Upon treatment with pervanadate , a tyrosine phosphatase inhibitor , human K19 ( hK19 ) was phosphorylated on tyrosine 391 , located in the ' tail ' domain of the protein . - pubmed
This tyrosine residue is not phosphorylated under basal conditions , but becomes phosphorylated in the presence of Src kinase in vitro and in cells expressing constitutively - active Src . - pubmed
An initial screen of osmolytes ( glycerol , sucrose , polyethylene glycol , 2,4 - methylpentanediol , glutamic acid , arginine , glycine , proline , betaine , sarcosine , and trimethylamine N - oxide ) reveals that glycine , along with similarly structured compounds , betaine and sarcosine , protects AP - aldolase structure and activity from thermal inactivation . - pubmed
Consistent with these results , epigenetic analysis , such as pyrosequencing , bisulfate sequencing and methyl - specific polymerase chain reaction ( MSP ) , showed that the expression level of ZNF312b is highly dependent on the degree of DNA methylation in gastric cancer cell lines . - pubmed
In addition , by ChIP assay using anti - acetyl / methyl H3K9 antibodies , histone acetylation was shown to mediate the expression of the ZNF312b gene . - pubmed
Phosphorescence quenching was used to quantify Pm(O ( 2 ) ) in vivo at rest and across the rest - to - twitch contraction ( 1 Hz , 5 min ) transition in the spinotrapezius muscle . - pubmed
Development and validation of a RP - HPLC method for stability - indicating assay of gemifloxacin mesylate including identification of related substances by LC - ESI - MS / MS , 1H and 13C NMR spectroscopy . - pubmed
A validated stability indicating RP - HPLC assay of gemifloxacin mesylate was developed by separating its related substances on an Inertsil - ODS3V - C18 ( 4.6 x 250 mm ; 5 mum ) column using 0.1 % trifluoroaceticacid ( pH 2.5 ) and methanol as a mobile phase in a gradient elution mode at a flow rate of 1.0 mL / min at 27 C. The column effluents were monitored by a photodiode array detector set at 287 nm . - pubmed
Forced degradation of gemifloxacin ( GFX ) was carried out under acidic , basic , thermal , photolysis and peroxide conditions and the degradation products were separated and characterized by ESI - MS / MS , ( 1 ) H and ( 13 ) C NMR spectroscopy . - pubmed
Aliphatic carboxylates were generally millimolar hCA I and II inhibitors and low micromolar / submicromolar beta - CA inhibitors . - pubmed
ITPases are nucleoside triphosphate pyrophosphatases that clean the precursor pools of the non - canonical triphosphates of inosine and xanthine . - pubmed
ITPA is clinically important because a widespread polymorphism , 94C > A , leads to null ITPase activity in erythrocytes and is associated with an adverse reaction to thiopurine drugs . - pubmed
We further show that ITPA knockdown results in elevated mutagenesis in response to HAP treatment . - pubmed
This implies that individuals with defective ITPase are predisposed to genome damage by impurities in nucleotide pools , which is drastically augmented by therapy with purine analogs . - pubmed
Our results suggest that the hMGL cysteine mutant maintains the same overall architecture as wild - type hMGL . - pubmed
The results also underscore the superior nucleophilic nature of the reactive catalytic Ser(122 ) residue as compared to that of Cys(122 ) in the sol - S - hMGL mutant and suggest that the nucleophilic character of the Cys(122 ) residue is not commensurately enhanced within the three dimensional architecture of hMGL . - pubmed
LC / MS analysis of tryptic digests from sol - S - hMGL directly demonstrate covalent modification of this variant by NAM and AM6580 , consistent with enzyme thiol alkylation and carbamoylation , respectively . - pubmed
These data provide insight into hMGL catalysis , the key role of the nucleophilic character of Ser(122 ) , and the mechanisms underlying hMGL inhibition by different classes of small molecules . - pubmed
Z - VAD - FMK , an inhibitor of classical apoptosis , was unable to reverse the suppressive effect of anti - GPC . - pubmed
The results indicate that PPV does not induce type I interferon and that the NS2 protein of PPV could blocks dsRNA - induced IFN - beta promoter activation . - pubmed
The L / F - SD and L / F - WS supplemented diets had equal proportion of 500 ppm laminarin and fucoidan . - pubmed
The Y665F and N642H mutant constructs increased the transcriptional activity of STAT5 and tyrosine ( Y694 ) phosphorylation , which was also observed in patient samples . - pubmed
Analysis of molecular species composition revealed that around 50 % of 36 :x ( number of carbons in the acyl chains : number of double bonds ) MGDG was hydrolysed and diacylglycerol ( DAG ) used for phospholipid synthesis , while another MGDG fraction was converted into digalactosyldiacylglycerol via the DGD1 / DGD2 pathway and subsequently into oligogalactolipids by SFR2 . - pubmed
The accumulation of oligogalactolipids and PI in the chloroplast and extraplastidial membranes , respectively , increases the concentration of hydroxyl groups and enhances the ratio of bilayer- to non - bilayer - forming lipids , thus contributing to protein and membrane stabilization . - pubmed
METHODS : Hazard ratios and 95 % confidence intervals ( 95 % CI ) from Cox proportional hazards analyses of mortality versus metabolic equivalent hours per day of exercise ( MET - hours per day , where 1 MET = 3.5 mL O2 kg min , or approximately 1 - km run ) . - pubmed
Common polymorphism ( 81Val>Ile ) and rare mutations ( 257Arg>Ser and 335Ile>Ser ) of the MC3R gene in obese Polish children and adolescents . - pubmed
NSAIDs are effective anti - inflammatory and analgesic drugs by virtue of their ability to inhibit biosynthesis of prostaglandins at the level of the cyclooxygenase enzyme . - pubmed
Development of novel N - linked aminopiperidine - based mycobacterial DNA gyrase B inhibitors : scaffold hopping from known antibacterial leads . - pubmed
Omarigliptin ( MK-3102 ): a novel long - acting DPP-4 inhibitor for once - weekly treatment of type 2 diabetes . - pubmed
AIP1 is a major cofactor of the actin depolymerizing factor / cofilin in eukaryotes , promoting actin depolymerizing factor / cofilin - mediated actin disassembly . - pubmed
This protein was purified after three chromatographic steps on cation exchange CM - Sepharose fast flow , size - exclusion column Sephacryl S-300 and anion exchange Capto Q. Bothropoidin was homogeneous by SDS - PAGE under reducing and non - reducing conditions , and comprised a single chain of 49,558 Da according to MALDI TOF analysis . - pubmed
Bothropoidin induced haemorrhage [ minimum haemorrhagic dose ( MHD ) = 0.75 microg ] , inhibited platelet aggregation induced by collagen and ADP , and interfered with viability and cell adhesion when incubated with endothelial cells in a dose and time - dependent manner . - pubmed
Mn - superoxide dismutase ) , ( 3 ) the production of reactive oxygen and nitrogen species under stress conditions was markedly decreased , with the significant alleviation of concomitant protein oxidation . - pubmed
CONCLUSION : With few exceptions like ALDH2 * 2 , the contribution of individual genetic variants to the risk for alcohol - related disorders is small . - pubmed
PFs for metiram and pyraclostrobin related to washing and oil dipping were 0.47 and 0.41 , and 0.78 and 0.63 at single dose ( SD ) and double dose ( DD ) , respectively . - pubmed
aureus is influenced by acquisition of the methicillin resistance gene mecA. - pubmed
Recent findings indicate that endothelial NOS and reactive oxygen species ( ROS ) are significantly increased during hypoxic stress and are modulated by antioxidants . - pubmed
However , the influence of antioxidants and steroids on nNOS upregulation by hypoxia is largely unknown . - pubmed
Hypoxia exposure ( dissolved oxygen , DO : 1.7 mg / L for 2 and/or 4weeks ) caused significant increases in hypothalamic nNOS mRNA , protein and its neuronal expression . - pubmed
In contrast , treatments with Nomega - nitro - L - arginine methyl ester , a competitive NOS - inhibitor , or vitamin E , an antioxidant , prevented the upregulation of O2 ( - ) production and nNOS expression in hypoxia - exposed ( DO : 1.7 mg / L for 4 weeks ) fish . - pubmed
Taken together , the results suggest that upregulation of nNOS and ROS in the croaker hypothalamus in response to hypoxia is influenced by antioxidant and overall estrogen status . - pubmed
Carbonic Anhydrase Activity Monitored In Vivo by Hyperpolarized 13C - Magnetic Resonance Spectroscopy Demonstrates Its Importance for pH Regulation in Tumors . - pubmed
Carbonic anhydrase buffers tissue pH by catalyzing the rapid interconversion of carbon dioxide ( CO2 ) and bicarbonate ( HCO3 ( - ) ) . - pubmed
We assessed the functional activity of CAIX in two colorectal tumor models , expressing different levels of the enzyme , by measuring the rate of exchange of hyperpolarized ( 13 ) C label between bicarbonate ( H ( 13 ) CO3 (- ) ) and carbon dioxide ( ( 13 ) CO2 ) , following injection of hyperpolarized H ( 13 ) CO3 (- ) , using ( 13 ) C - magnetic resonance spectroscopy ( ( 13 ) C - MRS ) magnetization transfer measurements . - pubmed
( 31 ) P - MRS measurements of the chemical shift of the pH probe , 3 - aminopropylphosphonate , and ( 13 ) C - MRS measurements of the H ( 13 ) CO3 (-)/( 13 ) CO2 peak intensity ratio showed that CAIX overexpression lowered extracellular pH in these tumors . - pubmed
Specifically , we review how even single amino acid changes in T cell epitopes can lead to loss of binding to major histocompatibility complex ( MHC ) , lack of cross - reactivity , or antagonism and immune interference , where simultaneous or sequential stimulation with related variants of the same T cell epitope can cause T cell anergy or the conversion of effector to immunosuppressive T cell phenotypes . - pubmed
Preparation and Biological Activity of New Collagen Composites , Part I : Collagen / Zinc Titanate Nanocomposites . - pubmed
The aim of this investigation was to develop new antimicrobial collagen / zinc titanate ( ZnTiO3 ) biomaterials using a sol - gel cryogenic draying technology in keeping the native collagen activity . - pubmed
Three mechanisms of the antimicrobial action were supposed , including ( i ) mechanical demolition of the cell wall and membrane by the crystal nanoparticles of the ZnTiO3 entrapped in the collagen matrix , ( ii ) chelation of its metal ions , and ( iii ) formation of free oxygen radicals due to the interaction between the microbial cells and antimicrobial agent . - pubmed
Moreover , PM2.5 increased the levels of intracellular reactive oxygen species ( ROS ) and the nuclear translocation of NF - E2 - related factor-2 ( Nrf2 ) . - pubmed
This pathway is comprised of glucose phosphorylation via polyphosphate glucokinase , NADH generation catalyzed by glucose 6 - phosphate dehydrogenase ( G6PDH ) and 6 - phosphogluconate dehydrogenase ( 6PGDH ) , electron transfer from NADH to the anode , and glucose 6 - phosphate regeneration via the non - oxidative pentose phosphate pathway and gluconeogenesis . - pubmed
In breast carcinoma cells , high levels of hyaluronan ( HA ) and its CD44 receptor are frequently associated with alteration in estrogen signaling . - pubmed
Albeit without effect on ERalpha expression and estradiol binding properties , HAS2 overexpression increased ERalpha Ser118 phosphorylation as well as transcriptional activity of estrogen in an ERE - luciferase reporter gene assay . - pubmed
However , HAS2 overexpression induced partial silencing of E2 driven - genes without affecting the magnitude of regulation by estradiol . - pubmed
This effect was associated with half - reduction in the activity of nuclear histone deacetylases ( HDACs ) through a post - translational mechanism likely consecutive to the enhanced expression of the histone acetyl - transferase EP300 . - pubmed
In conclusion , increase in HA / CD44 interactions may contribute , through an HDAC inhibitor - like and ER - independent mechanism , to the silencing of estrogen - driven genes in breast carcinoma . - pubmed
Methods rFVIIIFc and its isolated FVIII and Fc components were compared by the use of hydrogen - deuterium exchange mass spectrometry ( HDX - MS ) . - pubmed
Cytokine expression levels in ALS : A potential link between inflammation and BMAA - triggered protein misfolding . - pubmed
Furthermore , we propose a mechanism by which a known neurotoxin , beta - N - methylamino - l - alanine ( BMAA ) , may trigger this cytokine expression profile through motor neuron protein misfolding and subsequent NLRP3 ( nucleotide - binding domain ( NOD )- like receptor protein 3 ) inflammasome activation . - pubmed
Inhibition of STAT3 signaling pathway by ursolic acid suppresses growth of hepatocellular carcinoma . - pubmed
The effects of miRNA on the intracellular concentration and cytotoxicity of 5 - FU in CRC cells were measured by high performance liquid chromatography conjected tandem mass spectrometry ( HPLC - MS / MS ) and MTT methods , respectively . - pubmed
Statistical analysis showed that a polymorphism rs3742106 in the 3'-UTR of ATP - binding cassette subfamily C member 4 ( ABCC4 ) gene was significantly associated with the efficacy of 5 - FU / capecitabine - based chemotherapy in CRC . - pubmed
Opioid agonist treatment ( OAT ) with methadone or buprenorphine has strong evidence for treatment of this relapsing condition . - pubmed
In a recent randomized controlled trial , we demonstrated high rates of successful rapid referral from a syringe exchange programme ( SEP ) to treatment with methadone or buprenorphine , including actual treatment initiation . - pubmed
Median time to PSA progression was not reached with abiraterone versus 3.8 months for prednisone , attaining 58 % reduction in PSA progression risk ( HR = 0.418 ; p & lt ; 0.0001 ) . - pubmed
In 2013 , before switching to generic imatinib , all patients had reached MMR in accordance with European LeukemiaNet ( ELN ) Guidelines . - pubmed
Two different dry TOFA formulations ( silica carrier and palm oil coating ) were tested and showed performance effects similar to liquid TOFA . - pubmed
Searching for correlation between DNAmeth of the studied genes with demographic characteristics and baseline biochemical parameters of the studied population , we observed a correlation between IGF2 methylation and folate ( r = 0.44 , p = 0.003 ) . - pubmed
Joint associations of serum uric acid and ALT with NAFLD in elderly men and women : a Chinese cross - sectional study . - pubmed
BACKGROUND : Epidemiological evidence suggests sex difference in serum uric acid ( SUA ) and alanine aminotransferase ( ALT ) might be a potential explanation for the gender difference in prevalence of non - alcoholic fatty liver disease ( NAFLD ) . - pubmed
, gas production , pH , and short carboxylic acid concentrations , were compared to an untreated control at 96 h post inoculation . - pubmed
Seed mucilage RG - I with short side chains and unusual O3 substitution showed loose coils or star - like conformations . - pubmed
All four chemicals interfered with estradiol - mediated induction of PGR , NPPC , and GREB1 . - pubmed
The difficulty of accessing reference S - phosphorylated peptides ( pCys - peptides ) hampers progress in MS - driven cysteine phosphoproteomics , which requires targeted analytical procedures . - pubmed
Hen egg - white lysozyme was reduced with tris ( 2 - carboxyethyl ) phosphine ( TCEP ) followed by alkylation of cysteine with ( 3 - acrylamidopropyl ) trimethyl - ammonium chloride ( APTA ) and subsequent beta - elimination in aqueous Ba ( OH ) 2 to yield modified polypeptides containing multiple dehydroalanine ( Dha ) residues . - pubmed
The conjugate addition of thiophosphoric acid to Dha residues followed by trypsinolysis led to numerous D / L phosphocysteine - containing peptides , which were identified by higher - energy collisional - dissociation tandem mass spectrometry ( HCD - MS / MS ) . - pubmed
miR microarray analysis and PCR validation from CD4 + T cells showed treatment with resveratrol decreased the expression of several miRs ( miR-31 , Let7a , miR-132 ) that targeted cytokines and transcription factors involved in anti - inflammatory T cell responses ( Foxp3 and TGF - beta ) . - pubmed
Although the mutation analysis performed on DNA isolated from blood cells and progressed lesion samples confirmed the tumor to be germline BRCA wild - type , cisplatin monotherapy was administered based on the increasing evidence of safety and efficacy of platinum for breast cancer . - pubmed
In addition , vimentin expression was unaltered in comparison with the control , results that indicated an improvement in the CoCr biocompatibility with the ErGO on the material surface . - pubmed
Up to five size fractions of microcrystalline cellulose ( MCC ) , dibasic calcium phosphate ( DCP ) , and lactose monohydrate ( lactose ) in the range of < 125 microm up to the range of 355 - 500 microm were compressed into tablets and analysed with THz - TDS . - pubmed
This observation was more pronounced for DCP , followed by lactose and then MCC and the effect was more pronounced for larger compared to smaller initial particles . - pubmed
Suspending P2Y12 inhibitors while aspirin continued before elective CABG is recommended , as well as 12 months of DAPT following CABG . - pubmed
It is evident that the glutaraldehyde test gives a good idea of the immunoglobulin status of fatteners . - pubmed
Melatonin : deacetylation to 5 - methoxytryptamine by liver but not brain aryl acylamidase . - pubmed
Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover ; in addition , for the methylated nucleosides , higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells . - pubmed
Use of the TNF - alpha blocker , pentoxifylline , has also shown promise in lessening VOD . - pubmed
Some of the laboratory factors that did not show significant association with treatment response were the initial creatinine , hemoglobin , platelet count , lactate dehydrogenase , and total bilirubin . - pubmed
Synthesis and benzodiazepine receptor binding of 1H - pyrrolo [ 2,1 - C ][ 1,4 ] benzothiazines . - pubmed
Cyclosporin A inhibits the interleukin 2 receptor alpha chain gene transcription but not its cell surface expression : the alpha chain stability can explain this discrepancy . - pubmed
We have previously shown that CsA prevents the constitutive transcription of the IL2 gene in these cells . - pubmed
Here we show that as soon as 4 h after CsA addition , the transcription of the gene encoding the alpha chain ( p55 ) of IL2R was inhibited . - pubmed
CsA did not prevent this transient IL2 - dependent induction of IL2R alpha , but could still partially inhibit it . - pubmed
Once IL2 induction was over , CsA exerted its full inhibition . - pubmed
Thus , CsA does not seem to inhibit IL 2R alpha gene transcription simply by inhibition of IL2 synthesis . - pubmed
However , no modification of IL2R alpha expression on the cell surface could be detected after 48 h in the presence of CsA. - pubmed
This discrepancy between the effect of CsA on IL2R alpha expression as probed at the mRNA or the protein level can be accounted for by the stability of the IL2R alpha protein after synthesis . - pubmed
These results strongly suggest that the truncated gramicidins form a novel conducting channel which differs from the gramicidin head - to - head , single - stranded beta 6.3 - helical dimer ( " channel " ) in its conductance characteristic and its structure. - pubmed
When cells were lysed in the presence of 0.05 - 10 microM CaCl2 , the soluble 5 - lipoxygenase became associated with the particulate fraction . - pubmed
Ca2 + induced the membrane association of 5 - lipoxygenase when added into the incubation mixtures containing the membrane fraction with either the cytosolic fraction or the purified enzyme . - pubmed
5 - Lipoxygenase also bound to the microsomal - enriched fraction in the presence of Ca2 + . - pubmed
5 - Lipoxygenase binding to the membrane fraction was unaffected by pretreatment of the membranes with trypsin but was inhibited by treating with phospholipase A2 , suggesting that phospholipids are involved. - pubmed
, the pigmented area of the distal nerve ) the punctate laminin matrix is missing . - pubmed
By contrast , in the presence of l - PA or dl - PA new actinomycin components containing PA were synthesized at the expense of normally produced components . - pubmed
Although PA - containing actinomycins were formed as early as 6 h after the addition of 250 mug of l - PA per ml , it was not until 24 h postaddition that the relative percentages of actinomycin components produced approached a constant value . - pubmed
Ferredoxin - dependent phenylpyruvate synthesis by cell - free preparations of photosynthetic bacteria . - pubmed
Because of its extensive binding ( greater than 99 % ) to the protein fraction of the perfusate , the urinary excretion of carprofen was low an perfusate concentration - dependent . - pubmed
In contrast , the receptor - mediated uptake and degradation of mannosyl bovine serum albumin was increased three - fold . - pubmed
The effect of the Mo ( t ) supernatant was specific for macrophages and was abolished by preincubation of the supernatant with trypsin , which indicates that the active substances are protein in nature . - pubmed
The decrease in the uptake and degradation of MDA - LDL induced by preincubating the macrophages with Mo ( t ) supernatant appeared to result from a decrease in the number of receptors for this ligand at the cell surface . - pubmed
Esterase - like activity of human serum albumin : enantioselectivity in the burst phase of reaction with p - nitrophenyl alpha - methoxyphenyl acetate . - pubmed
CXD showed higher concentration than CEX , CED and CFT . - pubmed
The average recovery rates within 6 hours were 93.6 , 88.3 and 94.3 % respectively and they were similar to those of CEX , CED and CFT . - pubmed
Elimination of inhibitors of alkaline phosphatase from 2 - amino-2 - methyl-1 - propanol . - pubmed
When 2 - amino-2 - methyl-1 - propanol buffer solutions are extracted with chloroform , impurities inhibiting alkaline phosphatase are selectively removed and this source of variation is thereby greatly decreased . - pubmed
Augmentation of glucose transport into skeletal muscle by GLUT4 translocation to the plasma and T - tubule membranes can be mediated independently by insulin and by contraction / exercise . - pubmed
Fractions of 32 and 36 % sucrose exhibited the highest enrichment of GLUT4 and were independently responsive to insulin and exercise , respectively . - pubmed
The contraction- and insulin - sensitive GLUT4 pools also contained secretory component - associated membrane protein / glucose transporter vesicle triplet but not GLUT1 and caveolin . - pubmed
On the other hand , sedimentational analysis in sucrose velocity gradients revealed that the insulin - sensitive GLUT4 vesicles had a larger sedimentation coefficient than the exercise - sensitive vesicles . - pubmed
The fact that both children exhibited the rare Bombay blood group and were Lewis negative , each involving carbohydrates with different fucose linkages , suggested a possible defect in the fucose - containing ligand for E - and P - selectin , sialyl Lewis X ( SLe ( x ) ) . - pubmed
Adhesion of neutrophils to endothelial cells activated with interleukin-1 beta or histamine was markedly decreased ( & lt ; 5 % of control ) . - pubmed
Tubulin polymerization by paclitaxel ( taxol ) phosphate prodrugs after metabolic activation with alkaline phosphatase . - pubmed
Paclitaxel ( taxol ) phosphate derivatives BMY46366 , BMY-46489 , BMS180661 and BMS180820 were used to determine the ability of alkaline phosphatase to convert these water - soluble potential prodrugs to tubulin - polymerizing metabolites ( i.e. - pubmed
Compounds were treated up to 180 min with an in vitro metabolic activation system composed of 10 % bovine alkaline phosphatase in 0.2 M tris , pH 7.4 , or in 0.2 M glycine , pH 8.8 , plus 0.05 M MgCl2 . - pubmed
Pretreatment of 2 '- and 7 - phosphonoxyphenylpropionate prodrugs BMS180661 and BMS180820 with alkaline phosphatase for 30 to 120 min yielded relative initial slopes of about 20 to 100 % at test concentrations equimolar to paclitaxel . - pubmed
High - performance liquid chromatography / mass spectrometry of BMS180661 treated with alkaline phosphatase confirmed the production of paclitaxel from the prodrug . - pubmed
In contrast , 2 '- and 7 - phosphate analogs BMY46366 and BMY46489 treated with alkaline phosphatase were not active in tubulin assays . - pubmed
None of the paclitaxel phosphate prodrugs polymerized tubulin in the absence of metabolic activation . - pubmed
The differences in tubulin polymerization with metabolic activation may be related both to accessibility of the phosphate group to the enzyme and to anionic charge effects . - pubmed
These results demonstrate that certain paclitaxel phosphate prodrugs can be metabolized by alkaline phosphatase to yield effective tubulin polymerization . - pubmed
In fact , cAMP acted in two ways to stop IPC promyelocyte proliferation : ( 1 ) block in late G1 ( preventing new cells from entering DNA replication ) ; and ( 2 ) induction of apoptosis in S - phase . - pubmed
TEM experiments conducted on SLPF droplets cast from formic acid on amorphous carbon film demonstrated that these protein films have a microstructure formed of woven sheaves . - pubmed
TCR beta rearrangements involving gene segments from the V beta 1 gene family can be detected beginning on day 12 of development , while rearrangements involving the other family of V beta gene segments , V beta 2 , were first detected on day 14 of embryogenesis . - pubmed
VIP and preproVIP immunoreactivity was present in somata and nerve fibres of all central ganglia . - pubmed
The mean plasma concentration of human chorionic gonadotrophin and progesterone for the ectopic pregnancy group was significantly lower than that for the singleton delivery group ( P & lt ; 0.001 ) . - pubmed
This information was used to interpret the full oligosaccharide profiles of glycoproteins in a predictive manner based on arm specificity , linkage , and monosaccharide composition . - pubmed
Second , a serum IgG glycan pool was resolved into 20 peaks which were analyzed simultaneously by sequentially digesting the pool of sugars with exoglycosidase enzymes . - pubmed
The reproducibility of the separation system , the predictability of glucose unit values , and the quantitative response of the detection system for individual fluorescently labeled glycans also allowed the automatic analysis of neutral sugars using combinations of enzymes as in the reagent array analysis method ( RAAM ) . - pubmed
During the evolution of the autoimmune response to myelin basic protein ( MBP ) , at least two distinct lymphocyte populations arise , one that is specific for determinants within the protein antigen and another that is specific for the receptors displayed by the antigen - specific lymphocytes themselves . - pubmed
[ Apolipoprotein E and response to statins ] . - pubmed
The experimental strategy was to bypass upstream deficiencies by inducing normal , fertilization - like [ Ca2 +]i oscillations in fully grown , germinal vesicle ( GV ) stage oocytes and determine if the extent of CG exocytosis was restored to levels observed in mature , metaphase II ( MII)-stage eggs . - pubmed
These genes also showed differential responses in time course : more rapid induction was observed in the order of c - fos , hsp70 , and MT - IIA after exposure to Zn or Cd . - pubmed
By contrast , HSE - binding activity was detected in extracts from cells treated with Zn , Cd , Ag , and Cu . - pubmed
Agrin is a major heparan sulfate proteoglycan accumulating in Alzheimer ' s disease brain . - pubmed
However , while spread of GFP - VP22 was observed by examining intrinsic GFP fluorescence after methanol fixation , little spread was observed after PFA fixation , suggesting that the levels of the fusion protein in recipient cells were below the detection limits of intrinsic - fluorescence or that PFA fixation quenches the fluorescence of GFP - VP22 . - pubmed
We further considered whether elution of VP22 from methanol - fixed cells and postfixation binding to surrounding cells contributed to the increased detection of spread observed after methanol fixation . - pubmed
The extracted samples were analyzed on a jordi - gel DVB polyamine column ( operated under an acetonitrile / water gradient with water as the stronger mobile phase ) interfaced with a Finnigan TSQ 7,000 mass spectrometer . - pubmed
Allantoin and its internal standard ( 13C1 , 15N1 - allantoin ) were detected by use of single reaction monitoring ( SRM ) mode . - pubmed
Pharmacodynamic parameter estimates for postoperative vomiting were P0 ( the baseline probability of vomiting , with no opioid ) 0.115 , Pmax ( the maximal probability of vomiting due to morphine ) 0.997 , ED50 ( morphine dose that induces an effect equivalent to 50 % of the logit Pmax ) 0.18 mg / kg . - pubmed
Parameter estimates for overnight stay because of vomiting after morphine administration were P0 0.038 , Pmax 0.999 , ED50 0.369 mg / kg . - pubmed
Soluble N - ethyl maleimide - sensitive fusion protein attachment protein receptors ( SNAREs ) are core machinery for membrane fusion during intracellular vesicular transport . - pubmed
Comparing the PI arms at week 48 showed significantly higher levels of PT in the ritonavir - containing arms than in the indinavir arm ( p & lt ; 0.001 ) . - pubmed
In Experiment 1 , the effects of varying concentrations of BCAA and ARG in the TPN infusate , singly and together , were assessed : Group 1 , Standard TPN ( 19 % BCAA , 4.8 g ARG / L ) ; Group II , High BCAA TPN ( 35 % BCAA , 4.8 g ARG / L ) ; Group III , High ARG TPN ( 19 % BCAA , 9.6 g ARG / L ) ; Group IV , High ARG , High BCAA TPN ( 35 % BCAA , 9.6 g ARG / L ; Group V , chow and tap water ad libitum . - pubmed
Serum total bilirubin was significantly higher in Group I than in normals and in Groups II , III , and V. Serum lactate dehydrogenase levels were similar in normals and all 5 groups . - pubmed
The biochemical and physiological adaptations of plants to low temperatures include the post - translational activation and increased expression of enzymes of the sucrose synthesis pathway , the changed expression of Calvin cycle enzymes , and changes in the leaf protein content . - pubmed
Then , data from 8779 women screened using the quadruple test ( alphafetoprotein ( AFP ) , unconjugated estriol ( uE(3 ) ) , human chorionic gonadotrophin ( free beta - hCG ) , and inhibin - A levels with maternal age ) were used to determine the effect of smoking on the serum markers . - pubmed
Apomorphine , the catechol - derived dopamine D1 / D2 receptor agonist , is currently in use as an antiparkinsonian drug . - pubmed
The action of apomorphine can be mimicked by dopamine receptor ( D1 and D2 ) agonists or blocked by preincubation with D1 / D2 receptor antagonists . - pubmed
Increased mRNA expression levels of brain - derived neurotrophic factor and glial cell line - derived neurotrophic factor were also found in the apomorphine - treated mesencephalic cells along with concomitant protein expression increases in the conditioned medium . - pubmed
These results suggest that the apomorphine - modulated development of dopaminergic neurons may be mediated by activation of the dopamine receptor subtypes D1 and D2 thereby increasing the production of multiple growth factors . - pubmed
Cell viability and cytotoxicity were assessed by dimethylthiazolyldiphenyltetrazolium bromide ( MTT ) testing and quantification of lactate dehydrogenase . - pubmed
RESULTS : Neither keratin synthesis as a late marker of cell differentiation nor mitochondrial cell activity were influenced by either zinc compound . - pubmed
The synthesis of involucrin , an early marker of differentiation , was significantly increased by both zinc salts , ZnSO ( 4 ) being the more potent stimulator . - pubmed
Effect of reaction parameters on synthesis of citronellyl methacrylate by lipase - catalyzed transesterification . - pubmed
The methacrylate ester of citronellol was synthesized using various lipases as catalyst . - pubmed
The effect of different reaction parameters such as amount of lipase , solvent , temperature , and acylating agent on the conversion of citronellol to citronellyl methacrylate was studied . - pubmed
One - pot route to new alpha , alpha - difluoroamides and alpha - ketoamides . - pubmed
New alpha , alpha - difluoroamides ( 4a - d , 6a - d ) and alpha - ketoamides ( 5a - d , 7a - d ) result from one - pot reactions of alpha - ketoacids , RCOCO ( 2 ) H ( R = C ( 6 ) H ( 5 ) , CH ( 3 ) , CH ( 3 ) CH ( 2 ) , thienyl ) ( 1a - d ) with bis ( 2 - methoxyethyl ) aminosulfur trifluoride [ ( CH ( 3 ) OCH ( 2 ) CH ( 2 ))( 2 ) NSF ( 3 ) ] ( 2 ) ( Deoxofluor ) or diethylaminosulfur trifluoride [ ( CH ( 3 ) CH ( 2 ))( 2 ) NSF ( 3 ) ) ] ( 3 ) ( DAST ) . - pubmed
In the Danish cohort ( n = 306 ) of the Early Postmenopausal Intervention Cohort ( EPIC ) Study ( n = 1609 ) of oral alendronate ( ALN ) for prevention of postmenopausal osteoporosis , bone markers ( urine C - telopeptides of type I collagen ( uCTX ) , urine N - telopeptide cross - links of type I collagen ( uNTX ) , serum total osteocalcin measured by ELISA [ total OC ( ELISA ) ] , and serum total osteocalcin measured by RIA [ total OC ( RIA ) ] ) were measured at 6 - month intervals . - pubmed
ALPHA - AMYLASES AS CALCIUM - METALLOENZYMES . - pubmed
The drug salicylamide is an antagonist of the aryl hydrocarbon receptor that inhibits signal transduction induced by 2,3,7,8 - tetrachlorodibenzo - p - dioxin . - pubmed
The detrimental effects of TCDD are mediated via the aryl hydrocarbon receptor ( AhR ) , a transcription factor that regulates the expression of the carcinogen - activating enzymes cytochromes P-450 ( CYP ) 1A1 , 1A2 , and 1B1 . - pubmed
Salicylamide ( SAL ) , an analgesic drug , caused a potent and long - lasting inhibition of TCDD - induced CYP enzyme activity . - pubmed
Acetylsalicylic acid ( aspirin ) and the naturally occurring phytochemical salicylic acid had no effect on CYP activity . - pubmed
SAL inhibited the increase in CYP1A1 , - 1A2 , and - 1B1 mRNA levels that occurs on exposure to TCDD . - pubmed
TCDD - induced transcription of these genes was also inhibited by SAL , but not by aspirin or salicylic acid , as demonstrated by luciferase reporter assays . - pubmed
The transcription of the CYP1 family of genes is regulated by the interaction of TCDD - activated AhR with the xenobiotic - responsive element present in the promoter regions of these genes . - pubmed
As shown by electrophoretic mobility shift assay , SAL completely blocked the binding of TCDD - activated AhR to the xenobiotic responsive element . - pubmed
Also , SAL substantially blocked the binding of TCDD to the cytosolic AhR. - pubmed
These results demonstrate that SAL , a commonly used analgesic , is a potent inhibitor of AhR - mediated signal transduction , and may be an effective agent in the prevention of TCDD - associated disease . - pubmed
Activation of STAT3 and MAPK were manipulated by using dominant - negative STAT3 recombinant adenoviruses and a specific inhibitor of MAPK , respectively . - pubmed
RESULTS : Blocking of the MAPK pathway by the MAPK inhibitor PD98059 did not affect normal development of rods in retina explants or the suppression of their appearance by treatment with CNTF . - pubmed
In an effort to understand the mechanism , we also solved and report the structures of YbiV in complex with beryllofluoride ( BeF3- ) and aluminum trifluoride ( AlF3 ) , which have been shown to mimic the phosphorylated intermediate and transition state for hydrolysis , respectively , in analogy to other HAD phosphatases . - pubmed
Both structure and sequence homology indicate YbiV may be a sugar phosphatase , which is supported by biochemical assays that measured the release of free phosphate on a number of sugar - like substrates . - pubmed
Bicuculline , a GABA ( A ) receptor antagonist , was then injected ( 0.10 - 0.15 microl , 5 mM ) into the MRF through a stereotaxically placed glass micropipette . - pubmed
On the other hand , an injection of 0.10 - 0.15 microl of 5 mM muscimol , a GABA ( A ) receptor agonist , into the NRPv decreased the frequency of SLN - induced swallowing . - pubmed
However , these enzymes are activated by calcium or manganese , and as a result inhibited by chelators and by ATP , properties typical of neutral trehalases . - pubmed
Under these conditions the enzyme was neither activated by calcium nor inhibited by EDTA or ATP . - pubmed
The neutral activity was estimated in MES buffer at pH 6.5 , after subtracting the activity resistant to EDTA inhibition . - pubmed
AIMS : The main objectives of this study were to determine : ( 1 ) the relative roles of heavy episodic drinking ( HED ) , drinking frequency and drinking volume in explaining alcohol - related aggression and ( 2 ) whether drinking context variables ( i.e. - pubmed
usual drinking locations , typical drinking companions and extent of peer drinking ) confound or modify the relationship between HED and alcohol - related aggression or whether they predict alcohol - related aggression independently . - pubmed
Serum aminotransferase levels increased transiently 2 months after the cessation of immunosuppressive therapy ( prednisolone , azathioprine [ AZP ] , and CyA ) . - pubmed
Compared to soluble proteins , MHC classes I and II - restricted presentation of PLGA - MS - encapsulated proteins and peptides by MoDC was markedly prolonged and proteins were presented 50 - fold more efficiently on class I molecules . - pubmed
The apo and pyruvate - bound structures of PchB reveal that the enzyme is a structural homologue of chorismate mutases in the AroQalpha class despite low sequence identity ( 20 % ) . - pubmed
Determining the structure of PchB is part of a larger effort to elucidate protein structures involved in siderophore biosynthesis , as these enzymes are crucial for bacterial iron uptake and virulence and have been identified as antimicrobial drug targets . - pubmed
Ascorbic acid and its derivative induced a twofold increase in type VII collagen synthesis , and markedly increased the secretion of type VII collagen into the medium when compared with the control culture . - pubmed
For a given local Vo ( 2 ) , leg vascular conductance ( VC ) was five- to sixfold higher than arm VC , despite marked hemoglobin deoxygenation in the subclavian vein . - pubmed
A forbidden reflection at 7.792 keV near the Dy L3 absorption edge is identified as a quadrupolar ordering peak , and the quadrupolar order and a monoclinic structural distortion develop concomitantly below 12.3 K as second - order - type phase transitions . - pubmed
Hematoxylin - eosin staining and immunohistochemistry for Endonuclease G , Parvalbumin and Glial fibrillary acidic protein was performed after the ischemic insult . - pubmed
The magnitude of cell proliferation , measured by bromodeoxyuridine incorporation , was inversely proportional to the level of connexin32 expression . - pubmed
Positron - emission tomography - computed tomography ( PET / CT ) is rapidly being integrated into the imaging pathways of several different tumour types , most frequently using the glucose analogue 2 - [ ( 18 ) F ]- fluoro-2 - deoxy - D - glucose ( FDG ) . - pubmed
Integrated FDG - PET / CT combines functional and anatomical imaging to improve sensitivity and specificity of tumour detection . - pubmed
Abstract We tested the hypothesis of a cross - inhibition of oxytocin ( OT ) release by endogenous opioid peptides co - released with vasopressin ( VP ) . - pubmed
The effects of the opiate receptor antagonist naloxone were tested on neurohypophyseal VP release during dehydration , ethanol administration and sulphated cholecystokinin octapeptide ( CCK-8S ) application , assuming that the inhibition of pituitary OT release by endogenous opioids increases as neurohypophyseal VP output increases . - pubmed
Naloxone had no effect on VP release . - pubmed
Ethanol ( 10 % in saline ) administered intragastrically 50 min prior to decapitation and 20 min before subcutaneous naloxone ( 5 mg / kg ) resulted in the inhibition of VP output . - pubmed
The ethanol treatment resulted in a rise in plasma OT levels that was additional to the effect of naloxone . - pubmed
Peripheral CCK-8S administration induces an abrupt and selective secretion of OT . - pubmed
Blocking the opioid inhibition of OT release with naloxone resulted in a significant rise of OT compared to that with CCK-8S alone . - pubmed
No effect of CCK-8S and/or naloxone was found on VP plasma levels . - pubmed
The data indicate that opioid peptides co - released with VP ( like dynorphin ) may be responsible for cross - inhibition of OT release during dehydration . - pubmed
We reported , to our knowledge for the first time , that PS - G could promote neurite outgrowth of neurons cultured on the myelin substrates and inhibit the activation of RhoA. - pubmed
tumefaciens strain C58C1Rif containing plasmid pTDE4 harbouring neomycin phosphotransferase ( npt II , kanamycin resistance ) and beta - glucuronidase encoding genes . - pubmed
The Southern blot analysis has shown that 78 % kanamycin resistant plants contain gene encoding beta - glucuronidase . - pubmed
High magnetic field proton magnetic resonance spectroscopy ( ( 1 ) H - MRS ) provides a reliable measurement of GABA in specific regions of the brain . - pubmed
In the ACC , the GABA concentration was negatively correlated with the dose of the antipsychotics ( rs=-0.347 , p=0.035 ) . - pubmed
BACKGROUND : Both pramlintide and insulin pump waveforms separately provide improved post - meal glucose control . - pubmed
METHODS : Using the CGMS Gold ( Medtronics , Northridge , CA ) we measured the glucose response 0 - 4 h after the beginning of a meal and 15 min after the initiation of the insulin bolus wave and pramlintide ( 60 microg ) , bolus . - pubmed
RESULTS : With ST and C waves glucose fell approximately 40 mg / dL 0 - 2 h post - meal and then returned to baseline by the fourth hour . - pubmed
In comparison with other European countries , the use of RAAS antagonists is similar , there are more beta blockers and nitrates used , but there is less use of antithrombotic therapy ( especially anticoagulation ) and of statins . - pubmed
Epitaxial growth of graphitic carbon on C - face SiC and Sapphire by chemical vapor deposition ( CVD ) . - pubmed
We have measured the spin - polarized electron momentum density distributions of EuFe2 ( As0.73P0.27 ) 2 by magnetic Compton scattering ( MCS ) measurements . - pubmed
The MCS orbitalwise decomposition of the density distributions reveals that between 16 and 19 K , the spin - polarized Fe-3d character is enhanced ( as the ferromagnetic character supersedes superconducting character ) , where the resistivity shows a maximum , reentrant SC - like peak , at 18 K. The spin polarization of the Fe-3d orbital , enhanced by ferromagnetic Eu ions , suppresses the SC around 18 K , while at other temperatures the system indeed exhibits SC where the Fe-3d spin polarization is suppressed or collapses . - pubmed
OBJECTIVE : to develop a rapid and reliable real - time PCR to detect polymorphisms of angiotensinogen ( AGT ) , to compare the two methods of MS - PCR ( Mutagenically Separated PCR ) and real - time PCR to determine three polymorphisms of the angiotensinogen gene M235 T , the A ( -6 ) G and A ( -20 ) C. METHODS : the method of real - time PCR was developed on the PLC Roche LightCycler1 with SYBR Green I. We used two sense primers and a primer nonsense . - pubmed
omega - Transaminase ( omega - TA ) is an industrially important enzyme for production of chiral amines . - pubmed
This study aims to construct a mathematical model to simulate reductions in low - density lipoprotein ( LDL ) cholesterol in persons who combine the use of statins with a high intake of phytosterols/-stanols , e.g. - pubmed
METHODS AND RESULTS : The proposed model includes the cholesterol pool size in the liver and serum levels of very low - density lipoprotein ( VLDL ) cholesterol . - pubmed
Both an additional and a multiplicative effect of phytosterol/-stanol intake on LDL cholesterol reduction were predicted from the model . - pubmed
From the model , it was demonstrated that a daily intake of 2 g phytosterols/-stanols reduces LDL cholesterol level by about 8 % to 9 % on top of the reduction resulting from statin use . - pubmed
The additional decrease in LDL cholesterol caused by phytosterol/-stanol use at the recommended level of 2 g / d appeared to be similar or even greater than the decrease achieved by doubling the statin dose . - pubmed
Whereas in the NP breast , nuclei of epithelial cells were large and euchromatic , in the P breast they were small and hyperchromatic , showing strong methylation of histone 3 at lysine 9 and 27 . - pubmed
Downregulation of BCL2 by AT-101 enhances the antileukaemic effect of lenalidomide both by an immune dependant and independent manner . - pubmed
Lenalidomide , an immunomodulator , is clinically effective in CLL and can enhance the anti - CLL effects of CD20 targeting monoclonal antibody , rituximab . - pubmed
Here , we investigated the mechanism of immune - directed killing of lenalidomide in CLL and evaluated if concurrent targeting of CD20 and BCL2 can enhance this effect . - pubmed
This effect was further enhanced with pre - treatment of tumour cells with AT-101 ( a BH3 mimetic that functions as BCL2 antagonist ) . - pubmed
Neurogenesis was assessed by analysis of cells expressing NeuN , a neuronal marker , and 5 - bromo-2 '- deoxyuridine ( BrdU ) uptake . - pubmed
Immunohistochemical analysis showed that the number of BrdU / NeuN double - labeled cells in the dentate gyrus was significantly decreased 30 days after OBX . - pubmed
Chronic treatment with Comp.1 , Comp.2 or fluoxetine significantly increased the number of BrdU / NeuN double - labeled cells . - pubmed
Morphine - induced signaling via opioid receptors ( ORs ) in dorsal root ganglia ( DRG ) neurons , the spinal cord , and various brain regions has been shown to modulate gene activity . - pubmed
Overall , we reveal an important role of ELK-1 for morphine - dependent gene induction in DRG - derived cells and propose that ELK-1 and EGR-4 contribute to the effects of morphine on neuronal plasticity . - pubmed
RESULTS : mCPP significantly attenuated quinpirole - induced compulsive checking behavior by reducing vigor of checking ( indexed by frequency of checking and length of check ) and increasing rest after a bout of checking ( indexed by time to the next checking bout ) , but it did not affect focus on the task of checking ( indexed by recurrence time of checking and number of stops before returning to check ) . - pubmed
Ameliorative effects of mCPP may involve 5HT2A / 2C receptors in substantia nigra pars reticulata that inhibit expression of motor vigor . - pubmed
Moreover , integrin beta1 , through its abilities to activate latent TGFbeta1 and promote collagen production through focal adhesion kinase / rac1 / nicotinamide adenine dinucleotide phosphate oxidase ( NOX)/reactive oxygen species ( ROS ) , is essential for dermal homeostasis , repair and fibrosis . - pubmed
The gas - phase oxygen - hydrogen exchange reaction dynamics of O (( 3 ) P ) + i - C3H7 ( isopropyl ) H (( 2 ) S ) + CH3OCH3 ( acetone ) was first investigated by the vacuum - ultraviolet laser - induced fluorescence ( VUV - LIF ) spectroscopy in a crossed beam configuration . - pubmed
Histamine can exert effects through four different receptors , termed H1R - H4R. - pubmed
Intravenous injection of histamine was sufficient to elicit these responses , and similar to IgE - mediated anaphylaxis , loss of both H1R and H2R was necessary for complete protection . - pubmed
CONCLUSION : Our data demonstrate definitively that both H1R and H2R participate in the immediate systemic responses during histamine - associated pathophysiology and mechanistically support the utility of H2R - blocking therapeutics in alleviating symptoms of anaphylaxis . - pubmed
Disulfide bonds ( SS bonds ) are important post - translational modifications of proteins . - pubmed
Protein length also affects the SS bond frequency : the average number of SS bonds is positively correlated with the length for longer proteins ( & gt;200 amino acids ) , while for the shorter and less stable proteins ( & lt;200 amino acids ) this correlation is negative . - pubmed
Medium - sized proteins ( 250 - 350 amino acids ) indicated a high number of SS bonds only in Archaea which could be explained by the need for additional protein stabilization in hyperthermophiles . - pubmed
Denitrification activity was assayed using the chloramphenicol amended acetylene block method and bacterial communities were examined based on quantitative PCR and terminal restriction length polymorphisms of the nitrous oxide reductase ( nosZ ) and 16S rRNA genes . - pubmed
The aim of this study was to verify the clinical responses to Thai massage ( TM ) and Thai herbal compression ( THC ) for treating osteoarthritis ( OA ) of the knee in comparison to oral ibuprofen . - pubmed
In conclusion , TM and THC generally provided comparable clinical efficacy to oral ibuprofen after three weeks of treatment and could be considered as complementary and alternative treatments for OA of the knee . - pubmed
The change in the hypothalamic neuropeptide balance in response to leptin administration was associated with increased oxygen consumption , carbon dioxide production , and physical activity , which resulted in increased milk intake ( PN14 ) with no change in body weight . - pubmed
Here we use hydrogen / deuterium exchange to examine the interactions between a panel of bNAbs and native - like Env trimers ( SOSIP.664 trimers ) . - pubmed
In this study , we investigated the function of 6 - formylindolo ( 3,2 - b ) carbazole ( Ficz ) , a ligand of AHR , on IL-7 , colitis , and IEL phenotypes . - pubmed
Ficz also decreased CD8alphabeta(+ ) and CD8(+ ) IEL subpopulations , enhanced TCRgammadelta(+ ) IEL subpopulation , and reduced the percentage of activated CD4(+ ) and CD8(+ ) subpopulations . - pubmed
UVA - induced ROS and comet - like DNA formation were remarkably suppressed by EGT with a parallel inhibition of apoptosis , as evidenced by reduced DNA fragmentation ( TUNEL ) , caspase-9 /- 3 activation , and Bcl-2 / Bax dysregulation . - pubmed
Dose - dependent increases of antioxidant genes , HO-1 , NQO-1 , and gamma - GCLC and glutathione by EGT were associated with upregulated Nrf2 and downregulated Keap-1 expressions . - pubmed
Notably , augmented luciferase activity of ARE may explain Nrf2 / ARE - mediated signaling pathways behind EGT dermato - protective properties . - pubmed
This phenomenon was confirmed with suppressed nuclear Nrf2 activation , and consequently diminished antioxidant genes in cells treated with respective pharmacological inhibitors ( LY294002 , GF109203X , and N - acetylcysteine ) . - pubmed
ERK1 and ERK2 are the effector kinases of the ERK1 / 2 MAP - kinase signaling pathway , which plays a central role in transducing signals controlling cell proliferation , differentiation , and survival . - pubmed
Serum levels of PTH , ionised calcium , and creatinine were determined together with glomerular filtration rate ( GFR ) . - pubmed
On the contrary , nintedanib ( BIBF1120 ) was identified in large drug screening tests as a very specific inhibitor of certain tyrosine kinases , but no published data on preclinical tests existed until 2014 . - pubmed
perforatum extract , hypericin and hyperforin for 24 and 72 h. Then , a real - time PCR analysis was accomplished after incubating the cells with these products evaluating the relative CYP1A2 and CYP2D6 expression . - pubmed
Researchers at the University of California , Los Angeles , have shed light on mechanisms by which melanoma cells become resistant to PD-1 blockade with pembrolizumab . - pubmed
After 24 h treatment , cordycepin suppresses epithelial - mesenchymal transition and migration in ovarian carcinoma cells through inhibiting estrogen - related receptor ( ERR )- alpha . - pubmed
RESULTS : All changes in temperature for HVS , GAL , and TENS therapeutic electrical currents observed in our model system fall below the predefined 3 C. CONCLUSION : To the best of our knowledge , this is the first experimental based observation that prolonged exposure to a direct electrical current at therapeutic strength does not result in heating of metal titanium plates . - pubmed
Nearly 80 % of DIPGs harbor mutations in histone H3 genes , wherein lysine 27 is substituted with methionine ( H3K27 M ) . - pubmed
H3K27 M has been shown to inhibit polycomb repressive complex 2 ( PRC2 ) , a multiprotein complex responsible for the methylation of H3 at lysine 27 ( H3K27me ) , by binding to its catalytic subunit EZH2 . - pubmed
The block of P2X7R by JNJ47965567 significantly ( p<0.05 ) reverted the damage elicited by high glucose , detected as IL-1beta and LDH release . - pubmed
Overall , our findings suggest that the P2X7R represents an attractive pharmacological target to manage the early phase of diabetic retinopathy , and the compound JNJ47965567 is a good template to discover other P2X7R selective antagonists . - pubmed
bla OXA-48 carbapenemase was detected in seven O25b and one O16 isolates ; of those , one O25b - ST131 isolate was carbapenem - susceptible . - pubmed
Metallocluster extrusion requirements , interspecies MoFe - protein primary sequence comparisons and comparison of the primary sequences of the MoFe - protein subunits with each other have been used to assign potential P - cluster ( Fe - S cluster ) domains within the MoFe protein . - pubmed
In each beta unit of the MoFe protein , subunit domains , which include potential Fe - S cluster ligands Cys-62 , His-83 , Cys-88 and Cys-154 , and beta - subunit domains , which include potential Fe - S cluster ligands Cys-70 , His-90 , Cys-95 and Cys-153 , are proposed to comprise nearly equivalent P - cluster environments located adjacent to each other in the native protein . - pubmed
Results of these experiments show that MoFe - protein alpha - submit residues , Cys-62 and Cys-154 , are probably essential for MoFe - protein activity but that His-83 and Cys-88 residues are not . - pubmed
A hypothesized mechanism for everolimus - induced bone pain includes marrow edema and hyperemia from reduced intraosseous perfusion , which have been previously reported in association with mTOR inhibitor use . - pubmed
Glucose , insulin or lipid levels remained low , without the development of diabetes . - pubmed
However , levels of C - reactive protein ( CRP ) and matrix metalloproteinase-3 ( MMP-3 ) gradually increased thereafter and abatacept was initiated to prevent joint deterioration . - pubmed
Cholera toxin ( CT ) is composed of a disulfide - linked A1 / A2 heterodimer and a ring - like , cell - binding B homopentamer . - pubmed
Reduction of the A1 / A2 disulfide bond is required for holotoxin disassembly , but reduced CTA1 does not spontaneously separate from CTA2 / CTB5 : protein disulfide isomerase ( PDI ) is responsible for displacing CTA1 from its non - covalent assembly in the CT holotoxin . - pubmed
In contrast , high HNF-1beta expression was significantly associated with SLC3A1 expression , which plays a major role in HNF-1beta - triggered induction of reactive oxygen species in OCCCas , independent of abnormalities in both beta - catenin and MSI / MMR status . - pubmed
While it was long held that T cells were the primary mediators of multiple sclerosis ( MS ) pathogenesis , the beneficial effects observed in response to treatment with Rituximab ( RTX ) , a monoclonal antibody ( mAb ) targeting CD20 , shed light on a key contributor to MS that had been previously underappreciated : B cells . - pubmed
Using two expression profiles downloaded from the Gene Expression Omnibus database , differentially expressed genes ( DEGs ) between responders and non - responders to fluorouracil - based chemotherapy were identified . - pubmed
Following feature selection using two methods , a random forest classifier for response to fluorouracil - based chemotherapy with 13 DEGs was constructed . - pubmed
Simulating moxalactam dosage for extended - spectrum beta - lactamase - producing Enterobacteriaceae using blood antimicrobial surveillance network data . - pubmed
Objectives : Monte Carlo simulation ( MCS ) was used to evaluate optimal dosage for cefepime ( FEP ) , moxalactam ( MOX ) , and cefperazone / sulbactam ( CFZ / SBT ) against extended - spectrum beta - lactamase ( ESBL ) producers isolated from the Blood Bacterial Resistant Investigation Collaborative System . - pubmed
Conventional MOX and CFZ / SBT doses failed to reach 90 % PTA against isolates with MICs & gt;=8 mg / L and & gt;=4 mg / L , respectively . - pubmed
Paracetamol treatment produced an elevation in serum urea , uric acid , creatinine , C - reactive protein , renal nitric oxide , and lipid peroxide levels , and reductions in interleukin-10 , superoxide dismutase , and glutathione levels . - pubmed
BACKGROUND : During the initial scale up of ART in sub - Saharan Africa , prescribed regimens included drugs with high potential for toxicity ( particularly stavudine ) . - pubmed
The median BMI and CD4 count were 21.9 ( IQR : 19.6 - 24.8 ) and 188 cell / microL ( IQR : 65 - 353 ) respectively ; 27.5 % were started on stavudine , 46 % on zidovudine , and 26.5 % on a tenofovir containing regimens . - pubmed
We observed 6248 ART modifications in 4868/14,261 patients ( 34.1 % ) ; 1615 were due to toxicity , 1077 to treatment failure , 1330 to contraindications , and 1860 patients following WHO recommendation of phasing out stavudine and substituting with another NRTI . - pubmed
The application of norepinephrine ( NE ) , agonists of alpha - and beta - adrenergic receptors ( ARs ) or intracardiac nerves stimulation induced spontaneous AP in a form of periodical bursts or continuous firing . - pubmed
Acting through the muscarinic receptor , ACh reduces the magnitude of the potassium M - current , a hyperpolarizing current that builds up as neurons fire . - pubmed
Successful Treatment of Cosmetic Eyebrow Tattoos in Fitzpatrick III - IV With Picosecond ( 1,064 , 532 - nm ) Neodymium - Doped Yttrium Aluminum Garnet Laser With a Perfluorodecalin - Infused Patch : A Pilot Study . - pubmed
METHOD : In this study , we screened a small library and found that a flavonoid compound ( ZGM1 ) promoted the aggregation of beta - amyloid ( Abeta ) . - pubmed
Subsequently , we used dot blotting ( DB ) , transmission electron microscopy ( TEM ) , and thioflavin T fluorescence ( ThT ) measurements to study the aggregation of Abeta under the influence of ZGM1 . - pubmed
Enhanced Electrosynthetic Hydrogen Evolution by Hydrogenases Embedded in a Redox - Active Hydrogel . - pubmed
Hydrogenase enzymes offer a pathway for bioelectrochemically producing hydrogen that is advantageous over traditional platforms for hydrogen production because of low overpotentials and ambient operating temperature and pressure . - pubmed
We further show that Cc - PAA - mediated hydrogenases can operate at high faradaic efficiency ( 80 - 100 % ) and low overpotential ( -0.578 to -0.593 V vs. SHE ) . - pubmed
Specific activities of these hydrogenases in the electrosynthetic Cc - PAA assay were comparable to their respective activities in traditional methyl viologen assays , indicating that Cc - PAA mediates electron transfer at high rates , to most of the embedded enzymes . - pubmed
Interestingly , the PEI - PLA copolymer could effectively bind siRNA targeting PKM2 , and the obtained polyplex displayed much higher stability in serum than naked siRNA as determined by agarose gel electrophoresis . - pubmed
A diverse variety of CAMs assemble into complexes with glutamate and GABA receptors and regulate the targeting of these receptors to the cell surface and synapses . - pubmed
Relating to the molecular mechanism in Iron Deficiency Anemia ( IDA ) , hepcidin has a key role in iron homeostasis . - pubmed
It downregulates the iron exporter ferroportin , inhibiting both iron absorption and release . - pubmed
It can be used safely in various locations and different indications for preventingcomplications such as preventing dehiscence in revision surgeries , cerebrospinal fluid ( CSF ) fistula formation in the scalp , and wound breakdown in chronic corticosteroid use . - pubmed
Participants completed a laboratory - based psychosocial stress paradigm ( the Trier Social Stress Test - TSST ) pre- and post - intervention , throughout which cortisol and cardiovascular profiles were measured . - pubmed
Neither cortisol nor cardiovascular reactivity to the TSST was changed pre - to post intervention , either between or within groups . - pubmed
Reduction of CD73 protein resulted in significant suppression of GSC viability , proliferation and clonogenicity , whereas CD73 enzymatic activity exhibited negative effects only on GSC invasion involving impaired downstream adenosine ( ADO ) signalling . - pubmed
Furthermore , application of phosphodiesterase inhibitor pentoxifylline , a potent immunomodulator , effectively inhibited ZEB1 and CD73 expression and significantly decreased viability , clonogenicity , and invasion of GSC in vitro cultures . - pubmed
Given the involvement of adenosine and A3 adenosine receptor in GSC invasion , we investigated the effect of the pharmacological inhibition of A3AR on GSC maintenance . - pubmed
L - NAME ( non - selective nitric oxide synthase [ NOS ] inhibitor ) , aminoguanidine ( inducible NOS inhibitor ) , and 7 - nitroindazole ( neuronal NOS inhibitor ) were administered prior to modafinil . - pubmed
The main outcome variables included the percentage of necrotic area ( PNA ) in flap tissues , histopathological results , vascular endothelial growth factor ( VEGF ) immunohistochemical ( IHC ) staining , and nitrite concentrations . - pubmed
All NOS inhibitors significantly reversed the protective effect of modafinil ( p < 0.001 ) . - pubmed
Root canal filling was performed by the lateral compaction technique , using gutta - percha and an epoxy resin - based sealer ( AH Plus ) labeled with 0.1 % Rhodamine B dye . - pubmed
Ethanol - induced changes in activities of adenylate cyclase , guanylate cyclase and cyclic adenosine 3 ' , 5 '- monophosphate dependent protein kinase in the brain and liver . - pubmed
Maximum stimulation occurred at four days of incubation and 18 hours of pulse labeling with tritiated thymidine using 50 micrograms per milliliter of phytohemagglutinin and 1 microcurie of tritiated thymidine . - pubmed
The disruption of intercellular contacts , which accompanies cell dissociation and acquisition of motility , is correlated with a redistribution of E - cadherin , a Ca ( 2 +)-dependent cell adhesion molecule , over the entire cell surface and within the cytoplasm . - pubmed
The hemodynamic effects of ANF infusion ( 100 micrograms / h for 60 min ) were also examined in animals pretreated with the angiotensin - converting enzyme ( ACE ) inhibitor , captopril . - pubmed
Infusion of ANF attenuated the pressor responsiveness to exogenous AII and NE , but caused no significant change in the blood pressure increases produced by vasopressin . - pubmed
Infusion of captopril increased plasma concentration of renin ( PRC ) , and infusion of ANF produced no further change in PRC . - pubmed
The fragments were electrophoresed , transferred onto a nitrocellulose membrane and hybridized with two specific probes : pC21a for the CYP21 genes and pAT - A for the C4 genes . - pubmed
Two main types of CGD are known , an X - linked form which is normally associated with the absence of cytochrome b558 , a component of the membrane - associated reduced nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase which generates superoxide and an autosomal recessive form , in which cytochrome b558 is present , caused by the deficiency of a cytosolic factor required to activate NADPH oxidase . - pubmed
The origin of cerebellar NPY and possible colocalization with norepinephrine are discussed . - pubmed
The second example presents the balance of haemophilia A carrier detection rate performed in 30 obligatory carriers and 42 normal women by examination of F.VIII : C and VWF - Ag using universal discriminant . - pubmed
We conclude that immunocytochemistry on PLL - coated slides constitutes the method of choice for immunologic cell differentiation in CSF , which allows equivocal morphologic findings to be clarified . - pubmed
Although the receptor - mediated LDL uptake appeared to exert a strong influence on bile acid - independent bile flow , the receptor - independent uptake showed a significant relation with biliary bile acid excretion . - pubmed
Differences between the two mechanisms of LDL uptake were also evident in the biliary bile acid - cholesterol coupling , which was significantly stronger during receptor - independent than during receptor - dependent uptake of LDL . - pubmed
The effects of LDL uptake on bile secretion were modulated by the experimentally induced changes in both the content and composition of bile acids in the enterohepatic circulation . - pubmed
Cytochalasin B and Colchicine differ in their effect on the release of charge changing lymphokines . - pubmed
The amino acid composition and the amino - terminal sequence of the enzyme indicate that the protein is related to human pancreatic ribonuclease and to angiogenin , and that it may be identical with an eosinophil - derived neurotoxin and to a ribonuclease that has been isolated from urine . - pubmed
The catalytic activity of the liver ribonuclease and its sensitivity to iodoacetic acid inactivation also relate the enzyme to the pancreatic RNases , but the liver protein is clearly differentiated by immunological measurements . - pubmed
These include panretinal photocoagulation ( PRP ) , using the argon laser or xenon arc , for proliferative retinopathy , and focal photocoagulation for macular oedema . - pubmed
Other therapeutic modalities , such as hypophysectomy for severe retinopathy , remain controversial , while still others , such as rigorous blood glucose control and aldose reductase inhibitors , are currently under investigation . - pubmed
coli host cells , exonuclease III , was not utilized in repair of lethal H2O2 damage to the phage . - pubmed
HbO2 produced a dose - dependent reduction in AChE activity . - pubmed
In this concentration range MetHb and PSA had little effect on esterase activity . - pubmed
Estrogen and progesterone receptor content in breast tumor cytosols calculated with a pocket calculator . - pubmed
Activation of methionine synthetase by a reduced triphosphopyridine nucleotide - dependent flavoprotein system . - pubmed
With UDP - glucuronic acid prolonged incubation and variation of the composition of the incubation mixtures yielded equimolar amounts of azodipyrrole ( I ) and azodipyrrole beta - d - monoglucuronoside ( II ) after treatment of the incubation mixtures with the diazonium salt of ethyl anthranilate . - pubmed
After ammonolysis the conjugated azo - derivative ( II ) yielded d - glucuronic acid and the carboxylic acid amide of azodipyrrole , indicating transfer of a glucuronic acid residue to the carboxylic acid groups of bilirubin . - pubmed
The determination of human serum albumin by its specific binding of the anionic dye , 2 -( 4 '- hydroxybenzeneazo )- benzoic acid . - pubmed
By indirect immunofluorescence , the type 1 specific ( 6D4 ) and the group specific ( 1B5 ) reacted with a cytoplasmic antigen while the type 2 specific ( G3 ) reacted with a nuclear antigen . - pubmed
Mn2 + activated the adult ox enzyme 9 - fold and calf enzyme 22 - fold under optimal conditions ( no KCl ) . - pubmed
The vitamin K - dependent carboxylase from the dicoumarol - treated calf is apparently more tightly bound to the microsomal membrane than is the adult ox enzyme . - pubmed
Streptozotocin diabetes also caused a decrease in the cross - linking of reparative collagen as judged by the ratio of breaking strengths of skin incisions before and after formalin fixation . - pubmed
No significant associations of HLA - A or - B antigens with either group were observed after correction for the number of antigens tested . - pubmed
In the present report we show that protein kinase C ( PKC ) activity could be measured in extracts of distal neurites by an assay of the Ca ( 2 +)-dependent phosphorylation of a PKC - specific octapeptide substrate . - pubmed
We found that local application of 1 microM PMA for 24 h to distal neurites caused nearly complete down - regulation of Ca ( 2 +)-dependent PKC activity measured in this manner . - pubmed
We then investigated the effects of the recently available PKC inhibitors , calphostin C and chelerythrine , finding that , like PMA , these inhibited the growth of distal neurites when applied locally to them , and had no effect when applied to cell bodies and proximal neurites . - pubmed
However , the inhibition of neurite growth by calphostin C occurred at a concentration far below its IC50 value for protein kinase inhibition , and both calphostin C and chelerythrine inhibited distal neurite growth even in neurons pretreated with PMA . - pubmed
[ Study of the substrate specificity of T4 RNA ligase : interaction of the enzyme with ATP analogs modified through the ribose residue and with affinity sorbents ] . - pubmed
To elucidate the role of the ribose moiety in substrate binding , various ATP derivatives modified in ribose moiety were studied as probes for the T4 RNA ligase first stage reaction . - pubmed
Adenosine triphosphates attached to agarose were used as affinity adsorbent for purification of T4 RNA ligase . - pubmed
D1 , CDDP 100 mg / m2 ip D1 , CBDCA 300 mg / m2 i.v . - pubmed
The predicted amino acid sequence shows extensive homology to vitellogenin , the precursor of the lipovitellin complex , which has been shown by X - ray crystallography to contain a large lipid storage cavity . - pubmed
Results of segregation analysis are reviewed for six possibly related traits ( high sodium - lithium countertransport , low urinary kallikrein excretion , high fasting plasma insulin level , a fat pattern index , dense LDL subfractions , and body mass index ) . - pubmed
Oxygen sensitivity of second - phase secretion rate declined after 1 wk of in vitro culture : P50 was 13 mmHg after 1 wk and remained at 10 mmHg after 2 - 5 wk of culture . - pubmed
The reduction in insulin secretion is thought to be associated with the existence of pO2 gradients outside and inside the isolated islets , resulting in exposure of islet cells to low pO2 levels that decrease radially from the periphery to the core . - pubmed
Additionally , thromboelastography , prothrombin time , partial thromboplastin time , activated clotting time , fibrinogen , platelet counts , and hematocrit values were evaluated before the operation , after heparin reversal , after infusion of platelet rich plasma or control solution , and 2 hours after infusion . - pubmed
Perinatal hypothyroidism induced by methimazole treatment did not significantly alter the appearance of cerebral cortical G alpha o1 and G alpha o2 between days 1 and 22 postpartum . - pubmed
The boIL-5 coding sequence is 405 bp long , coding for a 15.2 kDa precursor protein of 134 amino acids ( aa ) . - pubmed
Interleukin-1 ( IL-1 ) and ibuprofen modulate the host response in different models after endotoxic challenge . - pubmed
Serum levels of IL-1 alpha , tumor necrosis factor - alpha ( TNF alpha ) , and interleukin-6 ( IL-6 ) were determined 1,2 , and 4 hr , post - LPS , and prostaglandin E2 ( PGE2 ) urine levels 4,8 , and 12 hr post - LPS , and a comparative mortality study was performed . - pubmed
IL-1 beta treatment provoked a reduction of IL-1 alpha , TNF alpha , and IL-6 without affecting PGE2 , while ibuprofen provoked a later increase of IL-1 alpha , TNF alpha , and IL-6 , with a decrease of PGE2 . - pubmed
Intravenous doses were as follows : rt - PA , 5 mg / kg over 0.5 h with 20 % of the total dose given as a bolus ; hirulog , 1 mg / kg bolus followed by 5 mg / kg over 1 h. MRI was performed at 2 , 3 , and 5 h following embolization . - pubmed
Significant cerebral hemorrhage was observed in one placebo , two rt - PA , and three rt - PA plus hirulog treated animals . - pubmed
We conclude that rt - PA plus hirulog improves cerebral perfusion but does not necessarily reduce cerebral injury . - pubmed
At this point the nuclear envelope becomes freely permeable to large ligands , as indicated by experiments with digitonin - treated cells and by the massive release of solubilized A - type lamins into the cytoplasm . - pubmed
M3S contains four changes : a hydrophilic substitution of L29S , two hydrophobic substitutions of S52I and Y56F , and a deletion of the N - terminal seven amino acids that is disordered in the structure of wild - type TNF - alpha . - pubmed
The low systemic toxicity of M3S appears to be the effect of the reduced and selective binding affinities for the TNF receptors , and the superior tumor - suppression of M3S appears to be the effect of its weak but longer antitumoral activity in vivo compared with wild - type TNF - alpha . - pubmed
Significantly high triglycerides and total cholesterol were observed in Groups I and II ( p & lt ; 0.001 ) and low high - density lipoprotein cholesterol in Group II ( p & lt ; 0.001 ) , when compared to controls . - pubmed
At 60 minutes during oral glucose tolerance test , higher insulin levels were seen in Groups I and II as compared to controls ( p = NS ) . - pubmed
Secreted pro - Atl ( 1256 amino acids ) is cleaved at residues 198 and 775 to generate a pro - peptide , amidase and glucosaminidase , respectively . - pubmed
Cells electroporated in the presence of anti - CaM antibodies had reduced influx of extracellular Ca2 + , with no change in release of Ca2 + from the internal stores . - pubmed
These observations suggest that T cell receptor engagement initiates Ca2 + influx by a pathway that likely includes CaM , which may in turn regulate IT . - pubmed
Influx of extracellular Ca2 + is required for cellular proliferation , and inhibition of CaM by pharmacological inhibitors reduced cellular proliferation . - pubmed
A combination of serum IgG precipitation by ammonium sulfate and yolk IgG extraction from egg was loaded in sodium dodecyl sulfate - polyacrylamide gel upon which the IgG was resolved by electrophoresis . - pubmed
Serum PRL and the concentrations of catecholamines , indoleamine , and their metabolites were measured by RIA and HPLC , respectively . - pubmed
Treatment with 5.0 mg deprenyl decreased the tumor diameter , tumor number , and serum prolactin ( PRL ) level . - pubmed
Treatment with 5.0 mg deprenyl enhanced the concentrations of norepinephrine ( NE ) and serotonin ( 5 - HT ) in the MBH and in the ST , and the concentration of dopamine ( DA ) in the MBH . - pubmed
These results suggest that the suppression of the development and growth of DMBA - induced mammary tumors by chronic deprenyl treatment may be mediated through alterations in the synthesis and metabolism of catecholamines and indoleamine in the MBH and inhibition of PRL secretion . - pubmed
Signal cross - contamination was approximately 0.2 % between individual coils , and simultaneous one- and two - dimensional spectra have been obtained from samples of fructose , galactose , adenosine triphosphate , and chloroquine ( 7 nmol of each compound ) . - pubmed
Since we had shown that Delta(2,4 ) double bonds could not be metabolized extra - peroxisomally to restore growth of the sps19Delta strain , we postulate that rat 3,2 - isomerase acted on the Delta(3 ) unsaturated metabolite of oleic acid by replacing the mutant 's missing activity from within the peroxisomes . - pubmed
These compounds were released enzymatically from their precursors by percolating the must through an immobilized tryptophanase column ( EC 4.1.99.1 ) , catalyzing an alpha , beta - elimination reaction on the S - cysteine conjugate . - pubmed
The volatile thiols were analyzed by GC - MS , as were the deuterated analogues that had been released from synthesized deuterated precursors and were added as internal standards . - pubmed
Ankyrins are spectrin - binding proteins that associate via ANK repeats with a variety of ion channels / pumps , calcium release channels and cell adhesion molecules . - pubmed
It consists of infinite , negatively charged , square , parallel columns bundled through N - H ... NC hydrogen bonds between NH ( 4 )(+ ) and terminal CN from the columns . - pubmed
Bicapped ( square face ) trigonal prismatic coordination geometries were observed for Ln ( III ) centers in 1 and 2 . - pubmed
Our previous work has identified two separate DNA - binding regions on NM23 - H2 / PuF : a sequence - dependent DNA - binding surface involving residues Arg34 , Asn69 , and Lys134 on the equator of the hexameric protein and a covalent DNA - binding site involving Lys12 located in the nucleotide - binding site , the site of the NDP kinase reaction . - pubmed
To understand the role of the nucleotide - binding site in the DNA cleavage reaction and to establish a connection between the nuclease and the NDP kinase activities , we used the known crystal structure of NM23 - H2 complexed with GDP as the basis for site - directed mutagenesis . - pubmed
We thus identified Arg88 and Arg105 as residues that are , in addition to Lys12 , critical for covalent DNA binding and DNA cleavage , as well as for the NDP kinase reaction . - pubmed
Another residue , Gln17 , was required only for DNA cleavage , and Tyr52 , Asn115 , and His118 were found to be essential only for the NDP kinase activity . - pubmed
We also show that nucleoside triphosphates but not nucleoside diphosphates inhibited the covalent DNA binding and DNA cleavage reactions , independent of phosphoryl transfer and the NDP kinase reaction . - pubmed
These findings collectively suggest that the binding modes of mononucleotides and duplex DNA oligonucleotides in the nucleotide - binding site differ , and that NM23 - H2 possesses multiple biochemical activities . - pubmed
Yolk oestradiol ( E2 ) increased seasonally while testosterone ( T ) decreased . - pubmed
For that purpose , we used WY14643 [ 4 - chloro-6 -( 2,3 - xylidino )- 2 - pyrimidinylthio acetic acid ] as a PPARalpha agonist , L-165041 and L-783483 as PPARbeta ligands , and 15 - deoxy - Delta ( 12,14 )- PGJ2 ( 15d - PGJ2 ) , troglitazone , and ciglitazone for PPARgamma . - pubmed
Cell viability against glutamate , hydrogen peroxide ( H ( 2 ) O ( 2 ) ) , and serum deprivation insults was determined using a calcein acetoxymethyl ( AM ) assay . - pubmed
However , the neuroprotective effects of those compounds are unlikely to be mediated through the PPARgamma for two reasons : ( 1 ) various concentrations of another PPARgamma agonist ( ciglitazone ) were not neuroprotective ; ( 2 ) by itself , PPAR exhibits a low affinity for DNA , and high - affinity binding requires heterodimerization with RXR , the 9 - cis - retinoic acid receptor ; administering 9 - cis - retinoic acid in conjunction with 15d - PGJ2 did not alter the neuroprotective effects of the latter . - pubmed
Our results demonstrate neuroprotective effects of 15d - PGJ2 and troglitazone that are likely independent of PPARgamma . - pubmed
Mechanical load significantly increased procollagen alpha1 ( I ) mRNA levels up to twofold above static controls after 24 h. This increase was completely abolished by the MEK 1 / 2 inhibitor U0126 , with no effect on basal levels . - pubmed
In contrast , SB203580 , a specific inhibitor of p38MAPK , enhanced both basal and stretch - stimulated levels of procollagen mRNA . - pubmed
Consistent with this finding , selective activation of the p38MAPK signalling pathway by expression of MKK6(Glu ) , a constitutive activator of p38MAPK , significantly reduced procollagen alpha1 ( I ) promoter activity . - pubmed
SB203580 - dependent increase in procollagen alpha1 ( I ) was accompanied by ERK 1 / 2 activation , and inhibition of this pathway completely prevented SB203580 - induced procollagen alpha1 ( I ) expression . - pubmed
Furthermore , the LC - ESI - TOF - MS analyses revealed that HALS-1 ( oligomeric ) was highly complex and consisted of at least five different mass series , while HALS-2 , which was assumed to be monomeric , contained two different mass series . - pubmed
In these studies , multiple conformations of the RNA polymerase II elongation complex were revealed by their distinct elongation potential and differing dependence on nucleoside triphosphate substrate . - pubmed
Using alpha - amanitin as a dynamic probe of the RNA polymerase II mechanism , we show that the most highly poised conformation of the elongation complex , which we interpreted previously as the posttranslocated state , is selectively resistant to inhibition with alpha - amanitin . - pubmed
This reaction , which is catalyzed by the protein - tyrosine kinase YwqD , activates the enzyme , while dephosphorylation of phosphotyrosine - YwqF by the phosphotyrosine - protein phosphatase YwqE reduces its enzyme activity . - pubmed
The UDP - glucose dehydrogenase reaction catalyzed by YwqF is inhibited by one of its substrates , UDP - glucose , and the extent of this inhibition seems to be reduced upon YwqF phosphorylation . - pubmed
We propose that this effect could at least partly account for the observed activation of YwqF induced by tyrosine phosphorylation . - pubmed
PURPOSE : In this study , we investigated the antitumor efficacy of thrombospondin-1 three type 1 repeats ( 3TSR ) , the antiangiogenic domain of thrombospondin-1 , in comparison and in combination with gemcitabine , in an orthotopic pancreatic cancer model . - pubmed
METHODS : In 2002 , we studied prescribing patterns of beta - blockers , calcium channel blockers , angiotensin converting enzyme ( ACE ) inhibitors , angiotensin - II antagonists and statins in the County of Funen , Denmark ( 180 general practices , 472,000 inhabitants ) . - pubmed
The overall structure of IDO shows two alpha - helical domains with the heme between them . - pubmed
A mutant analysis shows that none of the polar amino acid residues in the distal heme pocket are essential for activity , suggesting that , unlike the heme - containing monooxygenases ( i.e. - pubmed
, peroxidase and cytochrome P450 ) , no protein group of IDO is essential in dioxygen activation or proton abstraction . - pubmed
These characteristics of the IDO structure provide support for a reaction mechanism involving the abstraction of a proton from the substrate by iron - bound dioxygen . - pubmed
This study provides the structural basis for a heme - containing dioxygenase mechanism , a missing piece in our understanding of heme chemistry . - pubmed
Microtiter plates were sensitized with tumor necrosis factor alpha ( TNF - alpha ) and saturated with phosphate - buffered saline ( PBS ) containing 1 % bovine serum albumin ( BSA ) . - pubmed
Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2 , a molecule proposed to play a central role in interspecies quorum sensing . - pubmed
The negative element is contained within the overlapping dgt promoter , which is involved in purine metabolism . - pubmed
Oxygenase enzyme activity was determined in vivo using BD Oxygen Biosensor plates to measure oxygenase - mediated oxygen depletion in the presence of specific aromatic analytes . - pubmed
No significant signals were detected with hydrocarbons that were not substrates of the initial oxygenases . - pubmed
Thus , ethanol appears to prevent perseveration in performing needless instrumental behavior induced by repeated activation of D2 / D3 receptors . - pubmed
Apart from true double sensitization , IgE against cross - reactive carbohydrate determinants ( CCDs , alpha1,3 - fucosylated N - glycans ) with low clinical relevance is the most frequent and often only cause for the multiple reactivity . - pubmed
METHODS : Both gastroduodenal and small IP were assessed using an established three - sugar test , where urinary disaccharide excretion reflecting intestinal uptake was measured using HPLC . - pubmed
At the beginning and at the end of the study we recorded some clinical indicators such as BMI and WHR , some hormonal indicators such as testosterone , insulin , SHBG and DHEAS , as well as some metabolic indicators such as total cholesterol , triglycerides and HDL - C. RESULTS : We discovered a considerable reduction of 27,1 % in insulin and HOMA - IR ( 29,1 % ) , which indicates an improved insulin resistance . - pubmed
The decrease in testosterone and the increase in SHBG were not statistically significant . - pubmed
Although its mode of action is still not fully understood , ATO is known to induce cell apoptosis via generation of reactive oxygen species and activation of caspases . - pubmed
Photodynamic effects of steroid - conjugated fluorophores on GABAA receptors . - pubmed
We have shown that fluorescent , 7 - nitro-2,1,3 - benzoxadiazol-4 - yl amino ( NBD)-conjugated neurosteroid analogs photopotentiate GABA ( A ) receptor function . - pubmed
Our results are generally in agreement with the idea that steroids with activity at GABA ( A ) receptors are the most potent photopotentiators . - pubmed
Remarkably the crystal structure of this protein , solved to 1.2 A using x - ray crystallography , revealed that YtfQ binds a single molecule of galactofuranose in its ligand binding pocket . - pubmed
Modulatory effects of cAMP and PKC activation on gap junctional intercellular communication among thymic epithelial cells . - pubmed
BACKGROUND : We investigated the effects of the signaling molecules , cyclic AMP ( cAMP ) and protein - kinase C ( PKC ) , on gap junctional intercellular communication ( GJIC ) between thymic epithelial cells ( TEC ) . - pubmed
RESULTS : Treatment with 8 - Br - cAMP , a cAMP analog ; or forskolin , which stimulates cAMP production , resulted in an increase in dye transfer between adjacent TEC , inducing a three - fold enhancement in the mean fluorescence of coupled cells , ascertained by flow cytometry after calcein transfer . - pubmed
VIP , adenosine , and epinephrine which may also signal through cyclic nucleotides were tested . - pubmed
Stimulation of PKC by phorbol - myristate - acetate inhibited inter - TEC GJIC . - pubmed
CONCLUSIONS : Overall , our data indicate that cAMP and PKC intracellular pathways are involved in the homeostatic control of the gap junction - mediated communication in the thymic epithelium , exerting respectively a positive and negative role upon cell coupling . - pubmed
Fibroblastoid mast cells demonstrated increased chymase expression and activation with exaggerated spontaneous histamine release . - pubmed
A moderate increase of VEGF - C secretion was also observed in the presence of hypertonic doses of NaCl , which mimicked the matrix of chitin hydrolysate stock solution , and in the presence of chitin - binding lectin , WGA ( wheat germ agglutinin ) . - pubmed
These results suggest a novel function of chitin - derived oligosaccharides as VEGF - C stimuli . - pubmed
Since anti - apoptotic effect of ERK has not been elucidated clearly in DNA - damage - induced cell death , the role of ERK was examined in normal HEF cells treated with mild DNA damage using etoposide or camptothecin . - pubmed
PD98059 increased apoptosis and reduced DNA - damage - induced p21Waf1 / Cip1 / Sdi level . - pubmed
Depletion of p21Waf1 / Cip1 / Sdi induced cell death and PD98059 induced additional cell death . - pubmed
DNA - damage - induced increase in cytoplasmic localization and phosphorylation of threonine residues of p21Waf1 / Cip1 / Sdi was reversed by PD98059 . - pubmed
The histological grade and pyramidal neuronal density in the CA1 hippocampus were measured to quantitate the degree of hippocampal injury under thionin staining . - pubmed
Since endothelium - derived nitric oxide ( NO ) suppresses constitutively the release and vascular effects of thromboxane A ( 2 ) ( TXA ( 2 ) ) , NO - deficiency is often associated with activation of thromboxane receptors ( TP ) . - pubmed
In the present study we hypothesized that in the absence of NO , overactivation of the TP - receptor mediated cerebrovascular signaling pathway contributes to the development of vasomotion and CBF oscillations . - pubmed
Administration of the TP - receptor agonist U-46619 ( 1 microg / kg i.v . ) - pubmed
U-46619 significantly augmented the CBF - oscillations induced by L - NAME while inhibition of endogenous TXA ( 2 ) synthesis by ozagrel ( 10 mg / kg i.v . ) - pubmed
In isolated MCAs U-46619 in a concentration of 100 nM , which induced weak and stable contraction under physiological conditions , evoked sustained vasomotion in the absence of NO , which effect could be completely reversed by inhibition of Rho - kinase by 10 microM Y-27632 . - pubmed
CONCLUSION / SIGNIFICANCE : These results suggest that hypersensitivity of the TP - receptor - Rho - kinase signaling pathway contributes to the development of low frequency cerebral vasomotion which may propagate to vasospasm in pathophysiological states associated with NO - deficiency . - pubmed
Low HBsAg levels during peginterferon alfa-2a therapy were associated with high rates of posttreatment response . - pubmed
We demonstrate that electrospray ionization tandem ( triple quadrupole ) mass spectrometry ( ESI - MS / MS ) is an efficient method for monitoring steryl glucosides ( SG ) and acyl steryl glucosides ( ASG ) . - pubmed
These data demonstrate that ESI - MS / MS response per molar amount of sterol conjugate is similar among various molecular species of SG and ASG . - pubmed
METHODS : A cross sectional study was conducted in the north - east coast of Tanzania to investigate whether the changes in glycosylphosphatidylinositol ( GPI)-anchored complement regulatory proteins ( CD55 and CD59 ) contributes to malaria anaemia . - pubmed
In this case , the dissolution of IND ASD from water - soluble PVP and HPMCAS is almost instantaneous , resulting in an initial surge of IND concentration followed by a sharp decline due to the nucleation and crystallization events triggered by the rapid build - up of drug supersaturation . - pubmed
On the other hand , the dissolution of IND ASD from insoluble crosslinked PHEMA hydrogel beads is less rapid as it is regulated by a feedback - controlled diffusion mechanism , thus avoiding a sudden surge of supersaturation in the dissolution medium . - pubmed
The absence of an apparent decline in drug concentration during dissolution from IND - PHEMA ASD further reflects the diminished nucleation and crystallization events during IND dissolution from hydrogel - based solid molecular dispersions . - pubmed
Also , by selecting the appropriate particle sizes , immediate to controlled release of IND from IND - PHEMA ASD can be readily achieved as the release rate increases with decreasing PHEMA bead size . - pubmed
Furthermore , a robust physical stability has been demonstrated in IND - PHEMA ASD with no drug precipitation for up to 8 months at IND loadings below 16.7 % under direct open cup exposure to accelerated stability conditions ( 40 C/75 % RH ) . - pubmed
After acquisiting DICOM data of PET / CT / MR ( directed by radiotracer 18F - FDG etc . ) - pubmed
The results showed that the n - butanol extract had the highest anti - oxidant activity , and its anti - oxidant compounds were analysed by HPLC - DAD - ESI / MS(n ) and HPLC coupled with a post - column derivatisation ( PCD ) system supplied with DPPH , aluminium chloride or sodium acetate solutions . - pubmed
Results were classified as : normal glucose tolerance ( NGT ) or AGT ( impaired glucose tolerance [ IGT ] , CF related diabetes [ CFRD ] or indeterminate glucose tolerance [ INDET ] ) . - pubmed
We analyzed : OGTT ( glucose and insulin levels ) , nutritional status ( BMI - SD ) and lung function ( forced spirometry ) . - pubmed
In addition , r(m ) was modelled on the basis of detailed anatomical measurements to separate the importance of different serial resistances affecting CO ( 2 ) diffusion into chloroplasts . - pubmed
Calculated charge densities indicate that electrophiles should attack at C3 or O and nucleophiles at C2 or C4 in broad agreement with experimental observations . - pubmed
Complete inhibition of the enzyme by the inhibitor pepstatin A and the internal amino acid sequence classified the protein as an aspartic protease . - pubmed
The enzyme limitedly hydrolysed myoglobin between 1 - Met and 2 - Gly , 43 - Lys and 44 - Phe , and 70 - Leu and 71 - Thr . - pubmed
10 days after the start of using spironolactone and dabigatran bleeding was marked with decrease in hemoglobin levels to 69 g / l , creatinine level increases to 3.6 mg / dL ( glomerular filtration rate by MDRD 18 ml / min/1,73 m2 ) , and potassium to 5.5 mEq/ l. Against the background of the abolition of drugs normalization of renal function was marked . - pubmed
A common mechanism is the overexpression of ATP - binding cassette ( ABC ) efflux transporters in cancer cells such as P - glycoprotein ( P - gp / ABCB1 ) , multidrug resistance - associated protein 1 ( MRP1 / ABCC1 ) and breast cancer resistance protein ( BCRP / ABCG2 ) that limit the exposure to anticancer drugs . - pubmed
PP ( III ) is neither a substrate nor an inhibitor of pyrophosphatase , the enzyme that efficiently catalyses the hydrolysis of PP ( V ) . - pubmed
Toward one - pot lipase - catalyzed synthesis of poly ( epsilon - caprolactone ) particles in aqueous dispersion . - pubmed
The nature and amount of lipase , as well as the nature of the support material were identified as key parameters controlling ring - opening polymerization of epsilon - CL in aqueous dispersion . - pubmed
The body weight increase induced by avosentan was associated with CHF development ( P interaction=0.04 ) , whereas hemoglobin decrease was not ( P interaction=0.64 ) . - pubmed
Initially and following 4 weeks of tolcapone intake , the neurologist scored with Unified Parkinson ' s Disease Rating Scale parts I , II , IV , the nonmotor symptoms scale for Parkinson ' s disease and recorded the off time . - pubmed
Facile synthesis of brush poly ( phosphoamidate ) s via one - pot tandem ring - opening metathesis polymerization and atom transfer radical polymerization . - pubmed
Poly ( 2 -( dimethylamino ) ethyl methacrylate ) ( PDMAEMA)-based brush poly ( phosphoamidate ) s are successfully synthesized by a combination of ring - opening metathesis polymerization ( ROMP ) and atom transfer radical polymerization ( ATRP ) following either a commutative two - step procedure or a straightforward one - pot process using Grubbs ruthenium - based catalysts for tandem catalysis . - pubmed
Proton pump inhibitors omeprazole and lansoprazole contain chiral sulfur atom and they are administered as a racemate , i.e. - pubmed
After 12 months , there were significant differences in the serum 25 ( OH ) D concentration ( mean difference : 49.54 ; 95 % CI : 43.94 to 55.14 ) nmol / l ) and PTH levels ( mean difference : -1.02 ; 95 % CI : -1.67 to -0.38 pmol / l ) in the intervention group compared to placebo group . - pubmed
There was significant difference between treatment group in both serum 25 ( OH ) D and PTH . - pubmed
Here , we utilized a NOE - based approach to assign ( stereo- ) specifically labeled Ile , Leu , Val and Ala methyl labeled and uniformly ( 15 ) N - Phe and ( 15 ) N - Tyr labeled OmpX and calculated a refined high - resolution structure . - pubmed
We further used NOE information between protonated lipid head groups and side - chain methyls to determine the position of OmpX in the phospholipid bilayer . - pubmed
The protective HO-1 effects on placentation and fetal growth can be mimicked by the exogenous administration of carbon monoxide ( CO ) , a product of heme catalyzed by HO-1 . - pubmed
PURPOSE : Telaprevir inhibits CYP3A resulting in drug - drug interactions ( DDI ) of unprecedented magnitude . - pubmed
METHODS : We performed a static mechanistic DDI prediction to evaluate whether previously reported competitive inhibition of CYP3A by telaprevir and its diastereomeric metabolite - VRT-127394 is sufficient to explain the remarkable reduction in oral clearance observed with oral midazolam and tacrolimus . - pubmed
In comparing HLM incubations with and without a prior 30 - min exposure to telaprevir , a respective 4- and 11 - fold reduction in IC50 was observed with midazolam and tacrolimus as substrates . - pubmed
The sst2 somatostatin receptor , targeted by octreotide , is highly expressed in meningiomas . - pubmed
To investigate new therapeutic strategies , we evaluated the activity of everolimus ( mTOR inhibitor ) , BKM-120 and BEZ-235 ( new Pi3K / Akt / mTOR inhibitors ) , octreotide and a combined treatment ( octreotide plus everolimus ) , on cell proliferation , signaling pathways , and cell cycle proteins , respectively . - pubmed
Further , everolimus decreased cell viability of human meningiomas , but concomitantly , induced Akt activation , reducing the antiproliferative effect of the drug . - pubmed
The new Pi3 K inhibitors were not more active than everolimus alone , limiting their clinical relevance . - pubmed
In contrast , a clear cooperative inhibitory effect of octreotide and everolimus was observed on cell proliferation in all tested meningiomas , including WHO grades II - III . - pubmed
Octreotide not only reversed everolimus - induced Akt phosphorylation but also displayed additive and complementary effects with everolimus on downstream proteins involved in translation ( 4EB - P1 ) , and controlling cell cycle ( p27Kip1 and cyclin D1 ) . - pubmed
RESULTS : Body weight , visceral fat , BMI and food intake decreased significantly in OVX + AT + Vit D ( P & lt ; 0.001 ) ; whereas these variables increased significantly in OVX + C ( P & lt ; 0.001 ) and SHAM ( P & lt ; 0.023 ) groups . - pubmed
At the end of two - months of follow - up , we observed significant differences in TC , TG , HDL - C , LDL - C , glucose , insulin , and HOMA - IR in all groups . - pubmed
Total glutathione ( GSH + GSSG or GSx ) , reduced ascorbate ( AH2 ) , uric acid ( UA ) and antioxidant enzymes were determined in lung , heart and BALF immediately ( 0 h ) or 20 - h post 4 - h nose - only exposure to 0.0 , 0.25 , 0.5 and 1.0 ppm O3 . - pubmed
Two CVD strains ( JCR and SHHF ) had high basal levels of AH2 and GSx in BALF as well as high basal lung UA . - pubmed
Across all strains , high BALF GSx was only observed when high BALF AH2 was present . - pubmed
The results showed that animals fed with whole grain diets have lower food intake in comparison with the C diet , and decreased cecal content pH ( 7.06 vs. 6.33 ) and beta - glucosidase activity ( 1.66 vs. 0.21 mumol p - nitrophenol g(-1 ) cc h(-1 ) ) . - pubmed
Our group synthesized propane-2 - sulfonic acid octadec-9 - enyl - amide ( N15 ) , a novel peroxisome proliferator activated receptor alpha ( PPARalpha ) agonist . - pubmed
Simultaneous assessment of endogenous thiol compounds by LC - MS / MS . - pubmed
We reported a chemical derivatization- and LC - MS / MS - based approach to simultaneously determine thiol compounds including glutathione ( GSH ) , cysteine ( Cys ) , N - acetyl cysteine ( NAC ) , homocysteine ( Hcy ) , and cysteinylglycine ( CysGly ) in biological samples . - pubmed
Thiol - containing samples were derivatized with monobromobimane ( mBrB ) at room temperature , followed by LC - MS / MS analysis . - pubmed
PKA activation with a cAMP analogue alleviated the tumor vascular hyperpermeability , suggesting that the low PKA activity in the endothelial cells may be responsible for the tumor - tissue hyperpermeability . - pubmed
Motesanib , a kinase inhibitor for VEGF receptor , activated tumor endothelial PKA and reduced the vascular permeability in the tumor . - pubmed
Different measures to evaluate the efficacy of topiramate included subjective and objective assessment , photographs , the Skin Picking Scale modified after the Yale - Brown Obsessive - Compulsive Scale ( SPS - Y - BOCS ) , the Skin Picking Impact Scale , the Clinical Global Impressions - Improvement ( CGI - I ) and CGI - Severity scales , and the Beck Anxiety Inventory and Beck Depression Inventory . - pubmed
The samples were extracted by a simple protein precipitation method with methanol . - pubmed
The MS analysis was performed with multiple reaction monitoring ( MRM ) , and the transitions were set at m / z 753.3 427.0 for coumaroylspinosin and m / z 415.3 295.3 for IS , respectively . - pubmed
Here we show that the SETD8 / PR - Set7 methyltransferase , which catalyzes mono - methylation of histone H4 at lysine 20 ( H4K20me1 ) , suppresses nucleolar and mitochondrial activities to prevent cellular senescence . - pubmed
Upon anchoring the Ag NPs , ZnO NRs show enhanced band edge emission ( 380 - 400 nm ) and the emission is further significantly increased in Ag NP - anchored ZnO NTs . - pubmed
It is believed that the near - field coupling of SPR causes an optical enhancement of ZnO , whereas the bridging effect and hot - electron transfer to the conduction band of ZnO by plasmonic Ag NPs , anchored in close proximity , gives rise to a faster response of the photodetectors . - pubmed
RESULTS : In Experiment 1 , CBD alone produced no antinociceptive effects , while enhancing THC - induced paw pressure but not tail withdrawal antinociception 4 - 6h post - THC injection . - pubmed
CBD alone increased locomotor activity at 6h post - injection , but enhanced THC - induced hypolocomotion 4 - 6h post - THC injection , at lower THC doses . - pubmed
We summarize here how NPD1 elicits neuroprotection by up - regulating c - REL , a nuclear factor ( NF )- kappaB subtype that , in turn , enhances expression of BIRC3 ( baculoviral inhibitor of apoptosis repeat - containing protein 3 ) in the retina and in experimental stroke , leading to neuroprotection . - pubmed
The molecular mass of the purified TmrA was determined to be 44.5 kDa by SDS - PAGE and MALDI - TOF / TOF . - pubmed
The purified dehalogenase reductively dechlorinated CF to dichloromethane in vitro with reduced methyl viologen as the electron donor at a specific activity of ( 1.27 + - 0.04 ) x 103 units mg protein-1 . - pubmed
The most promising bioactive compound obtained from TW is celastrol . - pubmed
The beneficial effects of GIP were abrogated by inhibiting nitric oxide ( NO ) synthase , suggesting a possible mechanism mediated by NO . - pubmed
Furthermore , GIP dose - dependently increased NO production , whereas this effect was abolished by inhibiting AMP - activated protein kinase ( AMPK ) . - pubmed
GIP induced AMPK phosphorylation , which was abrogated by inhibiting phospholipase C and calcium - calmodulin - dependent protein kinase kinase but not by adenylate cyclase or liver kinase B1 , suggesting the existence of a calcium - mediated GIPR signaling pathway . - pubmed
Overall , we demonstrated the protective effects of GIP against peripheral arterial remodeling as well as the involvement of a calcium - mediated GIPR signaling pathway in vascular endothelial cells . - pubmed
The main factor of ascites formation is renal sodium retention due to activation of the renin - angiotensin - aldosterone system and sympathetic nervous system by the reduced effective volume secondary to splanchnic arterial vasodilation . - pubmed
The enzyme catalysis of AmyP and its mutants ( Q306A and E347A ) suggested that calcium ions contributed to the hydrolysis of cyclic structures in raw microalgae starch by a distinctive calcium - binding site Ca2 of AmyP. - pubmed
GlaxoSmithKline ( GSK ) has announced plans to buy Waltham , MA - based biotech Tesaro , maker of the once - daily oral PARP inhibitor niraparib ( Zejula ) , in a deal valued at $ 5.1 billion . - pubmed
Benzo [ d ] thiazol-2 ( 3H )- ones as new potent selective CB2 agonists with anti - inflammatory properties . - pubmed
In this work , we report a new series of selective CB2 agonists based on a benzo [ d ] thiazol-2 ( 3H )- one scaffold . - pubmed
Here , expression cloning with a cDNA expression library identified as an inhibitor of HIV-1 infection , a carboxy - terminally truncated form of human POZ / BTB and AT - hook - containing Zinc finger protein 1 ( PATZ1 ) , a transcriptional regulatory factor implicated in development and cancer . - pubmed
Experimental data of the tubulin polymerization inhibition assay was validated by molecular docking technique and the results exhibited strong hydrogen bonding interactions with amino acids ( ASN-101 , TYR-224 , ASN-228 , LYS-254 ) of tubulin . - pubmed
RESULTS : In the training cohort , hepatocellular carcinoma ( HCC ) , diabetes , total bilirubin ( TBIL ) , albumin ( ALB ) , alanine aminotransferase ( ALT ) , and serum creatinine ( Scr ) were independent predictors of in - hospital death . - pubmed
In this work , the low - temperature dehydration of fructose to HMF in different deep eutectic solvents ( DES ) was investigated . - pubmed
We found a very active and selective reaction system consisting of the DES tetraethyl ammonium chloride as hydrogen bond acceptor ( HBA ) and levulinic acid as hydrogen bond donor ( HBD ) in a molar ratio of 1:2 leading to a maximum HMF yield of 68 % after 120 h at 323 K. The DES still contained a low amount of water at the initial reaction , and water was also produced during the reaction . - pubmed
However , the nature of the HBA as well as the acidity of the HBD play an important role for the maximum achievable HMF yield . - pubmed
This was validated by measured yields in a DES with different combinations of HBD ( levulinic acid and lactic acid ) and HBA ( choline chloride and tetra - n - alkyl ammonium chlorides ) . - pubmed
In previous study , gallic acid ( GA ) , a part of EGCG , has shown anti - allergic inflammatory effect . - pubmed
Results : For in vitro , SG - SP1 reduced degranulation of mast cells by down - regulating intracellular calcium levels in a concentration - dependent manner . - pubmed
This inhibition was mediated by the reductions in serum histamine and interleukin-4 . - pubmed
Here , the crucial role of the ubiquitin lysine residue K27 in the process of histone H2A monoubiquitination mediated by the ubiquitin ligase RNF168 has been showed . - pubmed
The comparison of the turnover rate of the wild - type ubiquitin and its variant with a single functional lysine residue K27 suggests an important role of the ubiquitin deposition as a covalent conjugate with histone H2A in terms of the stability of the entire ubiquitinome . - pubmed
Nitric oxide production was measured by standard Griess reaction , arginase activity was determined by the measurement of urea , reactive oxygen species production was checked using NBT dye reduction assay and electron paramagnetic resonance spectroscopy , cytotoxic activity was estimated in MTT - assay . - pubmed
Membrane Cholesterol Regulates Endocytosis and Trafficking of the Serotonin1A Receptor : Insights from Acute Cholesterol Depletion . - pubmed
Our results show that the serotonin1A receptor exhibits agonist - induced clathrin - mediated endocytosis with a concentration - dependent inhibition in internalization with increasing concentrations of MbetaCD , which was restored upon cholesterol replenishment . - pubmed
Interestingly , subsequent to internalization under these conditions , serotonin1A receptors were re - routed toward lysosomal degradation , instead of endosomal recycling observed under normal conditions , thereby implicating membrane cholesterol in modulation of intracellular trafficking of the receptor . - pubmed
This raises the possibility of a novel cholesterol - dependent role of intracellular sorting proteins in GPCR trafficking . - pubmed
These results differ from our previous observations on the endocytosis of the serotonin1A receptor upon statin - induced chronic cholesterol depletion , in terms of endocytic pathway . - pubmed
We conclude that analysis of complex cellular trafficking events such as GPCR endocytosis under acute and chronic cholesterol depletion conditions should be carried out with caution due to fundamental differences underlying these processes . - pubmed
Full activity of thirteen A3 - corroles is achieved with four fluorine atoms present on the meso - phenyl ring reaching a selectivity index above 300 . - pubmed
To determine whether cholesterol side - chain cleavage enzyme ( CSCCE ) was deficient or absent in tubules , mitochondria from tubules , interstitium and whole testes were analyzed for CSCCE activity by measuring conversion of cholesterol - 26 - 14C to 14C - isocaproate ( + pregnenolone ) . - pubmed
The enzyme shares 85 % amino acid sequence identity with Fuc - TIII and 89 % identity with Fuc - TV but differs substantially in its acceptor substrate requirements . - pubmed
This response was abolished following pharmacological blockade of nicotinic receptors with hexamethonium and muscarinic receptors with atropine . - pubmed
A minimum of 6 weeks of co - treatment with TPA and rMuIFN - gamma ( zero time ) were necessary for demonstration of rMuIFN - gamma - dependent co - promotion . - pubmed
However , both doses of DFMO completely suppressed rMuIFN - gamma - dependent co - promotion . - pubmed
The simplest explanation for the increased sensitivity to restriction enzymes in the mutant is that the biochemical defect in XR-1 is not confined to the repair of ionizing radiation induced ends , but extends to DSBs that possess ligatable 3 '- hydroxyl and 5 '- phosphate ends as well . - pubmed
The rotation of cytochrome P-450 LM2 ( CYPIIB4 ) incorporated into large microsomal - like lipid vesicles was investigated by saturation transfer EPR using 15N- and 2H - substituted spin labels . - pubmed
32P - Post - labeling analysis allowed reproducible separation of DBF - DNA adducts and showed in addition the existence of several new adducts models of DBF . - pubmed
The allele - specific regulation of C4 expression and the androgen regulation of Slp expression are manifest only in some of the tissues where these genes are expressed . - pubmed
We show that these genes only affect the Slp expression in tissues where it is androgen - dependent . - pubmed
This indicates that the non - H-2 regulatory genes most likely act through interaction with the androgen regulation of Slp expression . - pubmed
The findings were : At 19 months of age CM showed higher testosterone and cortisol titers , higher adrenal tyrosine hydroxylase activities and higher seminal vesicles weights than IM . - pubmed
There is no apparent specificity for which strand of a duplex is initially cleaved by EcoB , nor is there specificity with respect to the composition of the 3'-terminal nucleotide formed on the DNA or the 3'- or 5'-terminal nucleotides of the acid - soluble oligonucleotides released during DNA cleavage . - pubmed
LDL - cholesterol , VLDL - cholesterol , and triglycerides during treatment periods with pindolol or atenolol , although there was a tendency to higher triglyceride levels during atenolol treatment . - pubmed
The serum HDL - cholesterol levels were significantly lower after 6 months of therapy with atenolol than before treatment , but HDL - cholesterol levels increased slightly during pindolol treatment . - pubmed
The ratio of HDL - cholesterol to total cholesterol decreased significantly during 6 months of treatment with atenolol . - pubmed
Serum insulin levels during oral glucose tolerance test at 120 min were decreased after pindolol therapy , but no significant changes were found in C - peptide levels during treatment periods . - pubmed
The immune response to a protein antigen is mainly in the IgG1 subclass , on the contrary the response to polysaccharide antigens is mainly IgG2 . - pubmed
Effect of obesity on ambient plasma glucose , free fatty acid , insulin , growth hormone , and glucagon concentrations . - pubmed
These amylases were expressed only when the organism was grown on maltose or other carbohydrates containing maltose units . - pubmed
Amylase synthesis was more severely repressed by glucose than by xylose . - pubmed
Amylases were produced in both wild - type and mutant strains when starch was added to cells growing on xylose but not when starch was added to cells growing on glucose . - pubmed
In both wild - type and mutant strains , glucoamylase and pullulanase were produced at high levels in starch - limited chemostats but not in glucose- or xylose - limited chemostats . - pubmed
Micro method for determination of blocked NH 2 - terminal amino acids of protein : application to identification of acetylserine of phosphoglycerate kinase and pyroglutamic acid of glucose 6 - phosphate dehydrogenase . - pubmed
It was found that adrenalectomy was followed by a decreased glucose - induced insulin secretion , glucose oxidation , calcium uptake , cAMP and insulin content without any remarkable change observed at the ultrastructural level . - pubmed
[ Prostaglandins and the kallikrein - kinin systems in the normal pregnancy - puerperium cycle and in hypertensive disease proper to pregnancy ] . - pubmed
Protein binding VPA ( equilibrium dialysis ) and FFA levels were determined on each sample . - pubmed
It was found that the chymotrypsin binding site that is not modified by mild oxidation with N - chlorosuccinimide ( Shechter et al . - pubmed
Structure of a mercaptan - thermolysin complex illustrates mode of inhibition of zinc proteases by substrate - analogue mercaptans . - pubmed
The structure of the complex of thermolysin and the inhibitor ( 2 - benzyl-3 - mercaptopropanoyl )- L - alanylglycinamide has been determined by X - ray crystallography at a resolution of 1.9 A and refined to a crystallographic residual of 18.4 % . - pubmed
The binding of this potent , specific inhibitor to thermolysin ( Ki = 7.5 X 10(-7 ) M ) serves as a model for the inhibition of zinc peptidases by substrate - analogue mercaptans . - pubmed
The study shows that the mercaptan inhibitor binds to thermolysin with the sulfur , presumably in the anionic form , tetrahedrally coordinated to the zinc and displacing a water molecule bound to the native enzyme . - pubmed
This is the first direct determination of the mode of binding of a mercaptan inhibitor to a zinc peptidase and confirms the geometry of binding expected on general grounds [ Ondetti , M. A. , Condon , M. E. , Reid , J. , Sabo , E. F. , Cheung , H. S. , & amp ; Cushman , D. W. ( 1979 ) Biochemistry 18 , 1427 - 1430 ; Nishino , N. , & amp ; Powers , J. C. ( 1979 ) Biochemistry 18 , 4340 - 4347 ] and inferred from previous spectroscopic studies [ Holmquist , B. , & amp ; Vallee , B. L. ( 1979 ) Proc . - pubmed
The inclusion in the growth medium of avidin , which almost irreversibly binds with biotin ( Kd , 10(-15 ) M ) , results in an increase in cellular biotin content and biotin enzyme activity over that seen when the cells are grown in a biotin - depleted medium . - pubmed
The addition of avidin - bound biotin to the growth medium led to a forty - fold increase in cellular biotin when compared to the inclusion of an equivalent amount of free biotin in the medium . - pubmed
Purified bovine factor H appeared to bind agarose - bonded heparin through its anion - binding domain and the binding was inhibited by the presence of free heparin or dextran sulfate . - pubmed
Calyculin A , the potent inhibitor of type 1 ( PP1 ) and type 2A ( PP2A ) phosphatases , has been employed in order to investigate the role of endogenously activated PP1 / PP2A in the signal - transduction pathway of platelet - activating - factor ( PAF)-stimulated platelets . - pubmed
Calyculin A alone caused an increase in protein phosphorylation in unstimulated platelets , with the detection of a number of newly phosphorylated proteins , whereas in PAF - stimulated platelets phosphorylation of the major substrates of protein kinase C and myosin light - chain kinase were no longer transient , but phosphorylation was sustained . - pubmed
PP1 / PP2A appear to play a role in Ca2 + homoeostasis , as inhibition of PP1 / PP2A caused an inhibition of Ca2 + mobilization and Ca2 + influx through the plasma membrane in PAF - stimulated platelets . - pubmed
The results are discussed in relation to the possible role of protein kinase C in mediating the events leading to the effects observed with calyculin A. - pubmed
These include a 12 - amino acid insertion in translation elongation factor 1 alpha and three small gaps in enolase . - pubmed
IgM antibodies to Gal ( beta 1 - 3 ) GalNac gangliosides appeared to have similar specificities since these were positively correlated and inhibitable by cross adsorption assays . - pubmed
Frequent amplification of a short chain dehydrogenase gene as part of circular and linear amplicons in methotrexate resistant Leishmania . - pubmed
The ltdh gene was amplified in all the mutants with the transport mutation of the first type , but not in all the mutants with a more pronounced decrease in the steady - state accumulation of MTX . - pubmed
In addition to these results with isoflurane , we found that 15 V stimulation and tail clamp produced comparable minimum alveolar concentration values for halothane and for desflurane . - pubmed
We have used selection in MPP+ to clone the cDNAs encoding two vesicular monoamine transporters , the first members of this novel gene family that now also includes the vesicular transporter for acetylcholine . - pubmed
This study investigates the reliability of glycated haemoglobin , measured by electroendosmosis or by affinity chromatography , fructosamine , and albumin adjusted fructosamine , as indices of glycaemic control in Type 1 diabetes complicated by chronic renal failure . - pubmed
Calculation of an albumin adjusted fructosamine result failed to improve the correlation with blood glucose concentrations . - pubmed
Like the Grb2 adaptor protein , which is known to couple receptor tyrosine kinases to the small GTPase Ras , Nck is presumed to bind to tyrosine - phosphorylated proteins using its SH2 domain and to downstream effector proteins using its SH3 domain . - pubmed
The Pak family of serine / threonine kinases are known to be activated by binding to the GTP - bound form of Cdc42 or Rac1 , which are small GTPases of the Rho family that are involved in regulating the organization of the actin cytoskeleton . - pubmed
CONCLUSION : These results demonstrate that localization of Pak1 to the membrane in the absence of other signals is sufficient for its activation , and imply that the Nck adaptor protein could function to link changes in tyrosine phosphorylation of cellular proteins to the Cdc42 / Pak signaling pathway . - pubmed
In addition , co - injection of RPP13 - 1 and FITC - dextrans established that RPP13 - 1 can induce an increase in plasmodesmal size exclusion limit to a value greater than 9.4 but less than 20 kDa . - pubmed
The enzyme was highly oxygen sensitive and contained a redox - active flavin cofactor , but no iron centres . - pubmed
Analysis of the derived amino acid sequences revealed that the large subunit of the enzyme shares homology to glycyl radical enzymes , particularly near the predicted radical site . - pubmed
The radical - containing subunit of benzylsuccinate synthase is oxygenolytically cleaved at the site of the glycyl radical , producing the alpha'-subunit . - pubmed
The four genes for benzylsuccinate synthase and the activating enzyme are organized as a single operon ; their transcription is induced by toluene . - pubmed
Furthermore , NO levels were negatively correlated with the EC - SOD levels in common EC - SOD phenotype subjects . - pubmed
One amino acid substitution in the extracellular domain common to all known leptin receptor proteins results from this mutation . - pubmed
In each case , CGH successfully identified amplifications and/or deletions unique to the drug - resistant selected cell lines . - pubmed
The receptor - binding site is located in a C - terminal disulphide - bonded loop of each pilin monomer , but functional binding sites are displayed only at the tip of the pilus . - pubmed
The pharmacy - based supervised self - administration of methadone scheme ( SSA ) at the Camden and Islington Community Health Services Trust Substance Misuse Services ( CTDS ) in London has been found to be valuable by clients , pharmacists and keyworkers . - pubmed
CONCLUSIONS : Despite the lower ambient oxygen at high altitude and an increase in umbilical artery PI , the fetal circulation does not exhibit a ' brain sparing effect ' . - pubmed
Curcumin also decreases ERE ( estrogen responsive element )- CAT activities induced by 17 - beta estradiol . - pubmed
Under optimal conditions ( 0.1 M K+-Hepes , pH 7.3 ) GspS has synthetase activity with apparent K(m ) values for GSH , spermidine and MgATP of 242 , 59 and 114 microM respectively , and a k(cat ) of 15.5 s(-1 ) . - pubmed
Glutathionylspermidine is formed as end product and the enzyme lacks TryS activity . - pubmed
coli GspS , the recombinant enzyme also possesses amidase activity ( EC 3.5.1.78 ) , hydrolysing glutathionylspermidine to GSH and spermidine with a k(cat ) of 0.38 s(-1 ) and a K(m ) of 500 microM. - pubmed
GspS can also hydrolyse trypanothione at about 1.5 % of the rate with glutathionylspermidine . - pubmed
A single amino acid mutation ( Cys-79 - ->Ala ) is shown to ablate the amidase activity without affecting the synthetase activity . - pubmed
Physiologic data provided evidence that urea transport in red blood cells and kidney inner medulla was mediated by specific urea transporter proteins . - pubmed
One fragment , corresponding to residues 17 - 484 ( PhbN ' ) , contained the regulatory domain of the protein , but in missing the first 16 residues was devoid of the sole phosphorylation site of Phb , Ser14 ; the second fragment corresponded to residues 485 - 843 ( PhbC ) and contained the catalytic domain of Phb . - pubmed
Our results support the idea that amino acid residues proximal to the convertible serine of Phb are important for its specific interaction with the catalytic subunit of PhK , but that regions distinct from the convertible serine residue of Phb and from the catalytic domain of PhK may also be involved in the interaction of these two proteins . - pubmed
[ Behavior of various acetylenic quaternary ammonium salts with regard to serum and erythrocyte cholinesterase levels ] . - pubmed
[ ON THE ANTI - ARRHYTHMIC ACTION OF THE MAGNESIUM DISODIUM SALT AT EDTA ] . - pubmed
First , we tested the hypothesis that endotoxin blocks the estradiol - induced LH surge centrally , by preventing the GnRH surge . - pubmed
Given this evidence that endotoxin blocks the estradiol - induced LH surge at the hypothalamic level , we began to assess underlying central mechanisms . - pubmed
Although flurbiprofen abolished endotoxin - induced fever , which is a centrally generated , prostaglandin - mediated response , it failed to reverse blockade of the LH surge . - pubmed
Collectively , these results indicate endotoxin blocks the LH surge centrally , suppressing GnRH secretion via a mechanism not requiring prostaglandins . - pubmed
The sorption of phenol , m - nitrophenol ( m - NP ) , and o - cresol from water onto montmorillonite modified with cetyltrimethylammonium bromide ( CTAB ) in a column was studied . - pubmed
RESULTS : MARS therapy achieved a remarkable improvement in clinical symptoms and physic signs , accompanied with a significant decrease in serum bilirubin , ammonia , urea nitrogen , fragrant amino acid , endotoxin , IL-6 , and TNF - alpha levels ( 0.05 ) ; at the 72 hours bilirubin rebounding analysis . - pubmed
MARS treatments resulted in a significant decrease of bilirubin rebounding level in comparison with what PE did ( 0.01 ) , though the bilirubin removal efficiency between two groups was not statistically significant . - pubmed
We identified a degenerate retinoic acid response element ( RARE ) between -186 and -163 in the promoter region of the tbx2 gene . - pubmed
Previous studies have shown significant changes in dopamine and opioid receptors in the basal ganglia following administration of cocaine . - pubmed
Cocaine administration results in a significant increase in the number of opioid receptors in dopamine - enriched brain regions . - pubmed
These data also suggest that the molecular mechanism responsible for the up - regulation of microOrs in the caudate and putamen following cocaine exposure may depend , in part , on the co - existence of D2rs and micro - Ors in these cells . - pubmed
Four polymorphisms were evaluated , including the serotonin transporter HTTLPR promoter ins / del , GABAAalpha6 Pro385Ser , NPY Leu7Pro , and catalase 262C>T. Two of these , HTTLPR and GABAAalpha6 Pro385Ser , had been previously associated with LR and alcoholism in a preliminary study . - pubmed
CONCLUSIONS : This report strengthens the support for a relationship between the HTTLPR L and GABAAalpha6 Ser385 alleles to low alcohol LR and to alcoholism in a prospectively studied cohort evaluated for LR in young adulthood and before the onset of alcohol dependence . - pubmed
Dihydro - S1P , which binds to and activates S1P receptors but has no direct intracellular effect , suppressed neither apoptosis nor NF - kappaB activity . - pubmed
These results suggest that S1P inhibits male germ cell apoptosis independently of its receptors , possibly by inhibiting the transcription factor NF - kappaB and Akt phosphorylation . - pubmed
Several lines of evidence suggest that D - serine , an endogenous agonist of the glycine site on the NMDA receptors , might play a role in the pathophysiology of schizophrenia . - pubmed
RESULTS : Hydroxyproline levels for the three groups , F0 - 1 , F2 - 3 , and F4 , were significantly elevated as the fibrosis score increased . - pubmed
Several differences were noted between the amount of collagen present according to hydroxyproline measurement and the stages of fibrosis according to histology . - pubmed
CONCLUSIONS : Although increases in collagen content ( by hydroxyproline ) approximately paralleled increases in fibrosis stage ( by histology ) , there was considerable overlap and some discrepancies . - pubmed
A descriptive account is given of our most recent research on the actinide dioxides with the Advanced Light Source Molecular Environmental Science ( ALS - MES ) Beamline 11.0.2 soft X - ray scanning transmission X - ray microscope ( STXM ) at the Lawrence Berkeley National Laboratory ( LBNL ) . - pubmed
This , along with the time course of hemoglobin concentration and arterial O ( 2 ) saturation ( infrared oximetry ) allowed computation of BBB QaO ( 2 ) . - pubmed
MATERIALS AND METHODS : The -I - HDL kinetics were performed by infusion of ( 5.5.5 -( 2 ) H3 )- leucine for 14 h. The prebeta1 and alphaHDL were separated by gel filtration fast protein liquid chromatrography system ( FPLC ) . - pubmed
After inhibition with 5 mmol / L H ( 2 ) O ( 2 ) to FeSOD and Cu / ZnSOD , MnSOD enzyme activity was assayed with nitrotetrazolium chloride blue staining . - pubmed
This article focuses on the mechanisms by which hormone resistance develops , including the reactivation of androgen receptor during androgen deprivation therapy , the role of cancer stem cells , and the emergence of epithelial - mesenchymal transition cells , which have increased metastatic potential . - pubmed
The ethylene - dependent beta - xylosidase gene expression is hypothesized to relate to modification of cell walls in terminal buds during SD - induced growth cessation . - pubmed
Effects of Hydroxylamine on Photosystem II : I. - pubmed
Illumination of chloroplasts in the presence of NH ( 2 ) OH ( 2 mm ) leads to the destruction of all system II activities without affecting system I activity . - pubmed
The system II primary charge separation remains intact when incubated with this agent in the dark with release of one of the system II Mn pools and simultaneous destruction of O ( 2 ) evolving capacity . - pubmed
Dekalb 202 ) was subjected to O ( 2 ) stress , nodal roots with well developed cortical air spaces ( aerenchyma ) grew into the deoxygenated solution . - pubmed
METHODS : Urothelium - denuded bladder neck strips were dissected and mounted in organ baths containing a physiological saline solution ( PSS ) at 37 degrees C and gassed with 5 % CO ( 2 ) and 95 % O ( 2 ) , for isometric force recording . - pubmed
The relaxations to transmural nerve stimulation ( EFS ) or PACAP 38 were performed on strips precontracted with 1 microM phenylephrine ( PhE ) . - pubmed
EFS experiments were carried out in the absence and the presence of guanethidine ( 10 microM ) , atropine ( 0.1 microM ) , and N ( G )- nitro - L - arginine ( L - NOARG , 100 microM ) , to block noradrenergic neurotransmission , muscarinic receptors , and nitric oxide ( NO ) synthase , respectively . - pubmed
RESULTS : EFS ( 2 - 16 Hz , 1 ms duration , 20 sec trains , 75 mA current output ) evoked frequency - dependent relaxations which were reduced by the VIP / PACAP receptor antagonist PACAP ( 6 - 38 ) ( 3 microM ) , and by the neurotoxin of the capsaicin - sensitive primary afferents capsaicin ( 10 microM ) , and abolished by the neuronal voltage - activated Na (+ ) channel blocker tetrodotoxin ( TTX , 1 microM ) . - pubmed
The vasoactive intestinal peptide ( VIP ) receptor antagonist [ Lys(1 ) , Pro(2,5 ) , Arg(3,4 ) , Tyr ( 6 )]- VIP ( 3 microM ) failed to modify the EFS - induced relaxations . - pubmed
PACAP 38 ( 1 nM-1 microM ) induced concentration - dependent relaxations which were reduced by PACAP ( 6 - 38 ) , TTX and by the neuronal voltage - gated Ca ( 2 + ) channel inhibitor omega - conotoxin GVIA ( omega - CgTX , 1 microM ) . - pubmed
Fasting blood samples were analyzed for sex hormones , plasma glucose , insulin , C - reactive protein ( CRP ) , and adipocytokines . - pubmed
Insulin sensitivity ( assessed by homeostasis model assessment 2 modeling ) , androgen , and HDL cholesterol levels were significantly decreased , whereas total fat mass and LDL cholesterol , triglyceride , CRP , leptin , and fructosamine levels were significantly increased in untreated Klinefelter ' s syndrome patients . - pubmed
In treated Klinefelter ' s syndrome patients , LDL cholesterol and adiponectin were significantly decreased , whereas no difference in body composition was found in comparison with untreated Klinefelter ' s syndrome patients . - pubmed
The proteins were adsorbed on alkanethiol surfaces with different terminating groups and 50,000 best orientations of each protein were analyzed . - pubmed
Docking of the tubulin dimer revealed that the orientation favored from the applicational point of view can be found in a list of the first 14 results for monolayers with different terminating groups and that there may be a noticeable difference in tubulin dimer interactions with alkanethiol chains of various length . - pubmed
We further examined the effects of PPARgamma agonist 15 - deoxy - Delta12,14 - prostaglandin J2 ( 15d - PGJ2 ) , a naturally occurring PPARgamma ligand , to these endometrial carcinoma cell lines . - pubmed
We also examined the status of apoptosis and p21 mRNA expression of these endometrial carcinoma cell lines following addition of 15d - PGJ2 . - pubmed
We showed that PPARgamma agonist 15d - PGJ2 inhibited cell proliferation and induced p21 mRNA of endometrial carcinoma cell lines . - pubmed
These findings suggest that a PPARgamma ligand , 15d - PGJ2 , has antiproliferative activity against endometrial carcinoma . - pubmed
We found enhanced panicogenic sensitivity to sodium lactate and an increased intensity and a differential pattern of Fos expression after the administration of yohimbine or caffeine in TgNTRK3 . - pubmed
We quantified four monoesters , four oxidative DEHP metabolites , and two secondary metabolites of di - isononyl phthalate ( DiNP ) by a LC / LC - MS / MS method . - pubmed
1 - O - alkylglycerols reduce the stimulating effects of bFGF on endothelial cell proliferation in vitro . - pubmed
Here we showed that alkyl - Gro reduced the basic Fibroblast Growth Factor ( bFGF)-stimulated endothelial cell proliferation in a concentration - dependent manner . - pubmed
The effects of 0.5 and 5 ng / ml bFGF on growth were completely suppressed after 72 h - treatment by 50 microM alkyl - Gro . - pubmed
Since bFGF greatly increased ( +56%+/-15 ) the production of 1 - O - alkyl-2 - acyl - sn - glycerophosphate in alkyl - Gro - treated endothelial cells , our data suggest that the observed effects of alkyl - Gro could be mediated through PLD activation . - pubmed
Inhibition of bFGF - stimulated endothelial proliferation could support anti - angiogenic activity of alkyl - Gro . - pubmed
In the present study , DS demonstrated a high level of binding activity to receptor activator of NF - kappaB ligand ( RANKL ) and obstructed the binding of RANK to RANKL , determined using a quartz - crystal microbalance ( QCM ) technique . - pubmed
The Fe - P , Al - P and Oc - P have close relationships with the degree of pollution and may act as good indicators for environment pollution in Jiaozhou Bay . - pubmed
Recruitment of the p85 - p110 complex to receptors and adaptor proteins via the p85 SH2 ( Src homology 2 ) domains alleviates this inhibition , leading to PI3 K activation and production of PIP(3 ) ( phosphatidylinositol 3,4,5 - trisphosphate ) . - pubmed
Such a monomeric p85 would compete with heterodimeric p85 - p110 for pTyr ( phosphotyrosine ) recruitment , and thus repress PI3 K activity . - pubmed
Further , analysis of methylation status of proximal GC boxes using sodium bisulfite sequencing reveals differential methylation of proximal GC boxes in the two cell lines examined . - pubmed
Identification of a chloride ion binding site in Na +/ Cl - dependent transporters . - pubmed
Here , we use calculations of pK(A)s and homology modeling to predict the location of a functionally important Cl (- ) binding site in serotonin transporter and other Cl(-)-dependent transporters . - pubmed
The process was studied by monitoring the change in RB5 concentration and the intermediate products employing UV - visible spectrophotometry , ion chromatography ( IC ) , and gas chromatography/ mass spectrometry ( GC / MS ) techniques and depletion in total organic carbon ( TOC ) content as a function of irradiation time . - pubmed
The optimal conditions for DHCP generation were also established by autoclaving GA ( pH 2.3 ) at 121 degrees C for 3h . - pubmed
coli was restored when superoxide dismutase and catalase were added to the culture broth that contained DHCP . - pubmed
DATA SOURCES : Comprehensive search using keywords AVP receptor antagonists , hyponatremia , SIADH , conivaptan , tolvaptan , lixivaptan , nurse practitioner , and others was carried out using the National Library of Medicine ( PubMed ) Web site from which full - text articles were obtained . - pubmed
The inhibition of the proliferative burst in MNU - treated parous mammary glands coincided with the upregulation of Mnt , a Myc - suppressor , and the formation of histone deacetylase 1 / Mnt / Max complexes that unexpectedly contained c - Myc . - pubmed
These results suggest that the complexes also function as transcription repressors of the growth - related Myc targets in MNU - treated parous mammary glands . - pubmed
BACKGROUND AND AIM OF THE STUDY : Oral anticoagulation with vitamin K antagonists is effective for the prevention and treatment of thromboembolic events . - pubmed
The soil - applied treatments ( paclobutrazol and imidacloprid ) were applied once during the trial ( spring 2004 ) , and the foliarly applied treatments ( JA and harpin ) were applied each spring . - pubmed
In the present study , we examined the relevance of the apoptosis repressor with caspase recruitment domain ( ARC ) on cardiac myocyte survival and its underlying mechanisms in a model of Dox - induced cardiotoxicity . - pubmed
Proteasomal inhibitors partially rescued both Dox - induced downregulation of ARC protein and induction of apoptosis . - pubmed
Knockdown of endogenous ARC sensitised cardiomyocytes to undergo apoptosis upon treatment with Dox . - pubmed
In response to Dox , Bax translocated from cytosol to mitochondria where it resulted in dissipation of the mitochondrial membrane potential , cytochrome c release and activation of caspases - 3 and - 9 . - pubmed
In this study , we provide evidence for the protective role of anti - apoptotic ARC in Dox - induced cardiotoxicity , which makes this molecule an interesting target for future therapies . - pubmed
In all groups anesthesia was induced with sevoflurane , associated or not to fentanyl , according to the following regimens : G1 = sevoflurane plus 2.5 microg.kg-1 fentanyl ; G2 = sevoflurane plus 5 microg.kg-1 fentanyl ; G3 = sevoflurane plus 7.5 microg.kg-1 fentanyl ; and G4 = sevoflurane plus saline solution . - pubmed
The following parameters were monitored : SBP , DBP , HR , BIS , SE , RE , sevoflurane expired concentration ( EC ) and motor response to TI at three moments : M1 = immediately before induction ; M2 = immediately before tracheal intubation and M3 = one minute after tracheal intubation . - pubmed
In the present study , high - resolution solution NMR spectra were obtained of OmpX kept water - soluble by either amphipol A8 - 35 or the detergent dihexanoylphosphatidylcholine . - pubmed
During the first 6h post - dose , the bronchodilatory effect of aclidinium ( all doses ) on day 1 was comparable to that on day 29 . - pubmed
Design , synthesis and preliminary activity assay of 1,2,3,4 - tetrahydroisoquinoline-3 - carboxylic acid derivatives as novel Histone deacetylases ( HDACs ) inhibitors . - pubmed
Herein , we report a novel series of 1,2,3,4 - tetrahydroisoquinoline-3 - carboxylic acid derivatives as HDACs inhibitors . - pubmed
Within this series , five compounds , 13a ( IC(50)=0.58+/-0.10 microM ) , 7d ( IC(50)=1.00+/-0.16 microM ) , 8l ( IC(50)=1.06+/-0.14 microM ) , 7i ( IC(50)=1.17+/-0.19 microM ) and 7a ( IC(50)=1.29+/-0.15 microM ) possessed better HDACs inhibitory activity than Vorinostat ( IC(50)=1.48+/-0.20 microM ) . - pubmed
Core 2 beta1,6 - N - acetylglucosaminyltransferase ( C2GnT ) , which exists in three isoforms , C2GnT1 , C2GnT2 and C2GnT3 , is one of the key enzymes in the O - glycan biosynthetic pathway . - pubmed
Functional analysis of two single nucleotide polymorphisms in SLC30A2 ( ZnT2 ): implications for mammary gland function and breast disease in women . - pubmed
Zinc transporter 2 ( ZnT2 ) plays a major role in zinc ( Zn ) export from the mammary gland . - pubmed
Recently , we determined that ZnT2 is associated with secretory vesicles reflecting its role in Zn secretion during lactation . - pubmed
FluoZin-3 fluorescence illustrated increased lysosomal accumulation of Zn in cells expressing SNP1 concomitant with the abrogation of Zn secretion . - pubmed
In contrast , ectopic expression of SNP2 was associated with the expansion of cytoplasmic Zn pools , elevated reactive oxygen species , and increased Zn efflux . - pubmed
Taken together , our data indicate that polymorphic variants in ZnT2 distinctly alter mammary cell Zn metabolism . - pubmed
Several examined hyperkalemia associated with angiotensin - converting enzyme ( ACE )- inhibitor / angiotensin II receptor blocker ( ARB ) , diuretic ( potassium - sparing ) , and beta - blocker use . - pubmed
Finally , orthostatic hypotension was evaluated in those taking ACE - inhibitor / ARB , beta - blocker , or calcium - channel blocker therapy . - pubmed
The roles of p27 ( Kip1 ) , Bcl-2 , and Cdk1 in apoptosis induced by dasatinib and paclitaxel were assessed using a terminal deoxynucleotidyl transferase - mediated dUTP nick - end labeling ( TUNEL ) assay , siRNA knockdown of gene expression , transfection with Bcl-2 and Cdk1 expression vectors , and flow cytometry . - pubmed
The siRNA knockdown of Src , Fyn , or Abl1 enhanced paclitaxel - mediated growth inhibition in ovarian cancer cells compared with a control siRNA . - pubmed
The siRNA knockdown of p27 ( Kip1 ) decreased dasatinib- and paclitaxel - induced apoptosis compared with a negative control siRNA ( sub - G1 fraction , control siRNA vs. p27 ( Kip1 ) siRNA : 42.5 % vs. 20.1 % , difference = 22.4 % , 95 % CI = 20.1 % to 24.7 % , P = .017 ) . - pubmed
Studies with forced expression and siRNA knockdown of Bcl-2 and Cdk1 suggest that dasatinib - mediated induction of p27 ( Kip1 ) enhanced paclitaxel - induced apoptosis by negatively regulating Bcl-2 and Cdk1 expression . - pubmed
CONCLUSION : Inhibition of Src family and Abl kinases with either siRNAs or dasatinib enhances paclitaxel sensitivity of ovarian cancer cells through p27(Kip1)-mediated suppression of Bcl-2 and Cdk1 expression . - pubmed
Metabolism and pharmacokinetic studies of JPH203 , an L - amino acid transporter 1 ( LAT1 ) selective compound . - pubmed
Synthetic chemistry and in vitro screening efforts have afforded a variety of novel and highly hLAT1 selective compounds , such as JPH203 1 . - pubmed
The current work develops a robust LC / MS - MS method to monitor 1 and its major Phase II metabolite N - acetyl - JPH203 2 from biological samples . - pubmed
In 21 % of cases , analogous lesions involve the CD58 gene , which encodes a molecule involved in T and natural killer cell - mediated responses . - pubmed
Neither D1 - type ( SKF38393 ) nor D2 - type ( quinpirole ) agonist was effective when given alone . - pubmed
Twenty - four fully published articles were abstracted and summarised : 16 articles focused on five peptide receptor radionuclide therapy ( ( 111 ) In - DTPAOC , ( 90 ) Y - DOTALAN , ( 90 ) Y - DOTATOC , ( 90 ) Y - DOTATATE , and ( 177 ) Lu - DOTATATE ) and eight focused on ( 131 ) I - MIBG treatment . - pubmed
Thus , the capacity of endogenous free radical clearance can be of patho - physiological importance ; in this regard , the major reactive oxygen species defense machinery , the nuclear factor ( erythroid - derived 2 )- like 2 ( Nrf2 ) system needs to be precisely modulated in response to pathological alterations . - pubmed
METHODS : CE - MS analysis was performed on a 70 cm X 50 mum fused - silica capillary . - pubmed
The optimal MS parameters were as follows : ESI - MS analysis was performed in the positive mode ; 90 % methanol containing 0.2 % formic acid with a flow rate of 8 mul min(-1 ) was selected as the sheath liquid ; the flow rate and temperature of drying gas were 6 L min(-1 ) and 250 degree , respectively ; The nebulizing gas pressure was set at 5 psig ; The optimal values of fragmentor and ESI voltage were 100 V and 5 000 V , respectively . - pubmed
Type II natural killer T cells use features of both innate - like and conventional T cells to recognize sulfatide self antigens . - pubmed
Glycolipids presented by the major histocompatibility complex ( MHC ) class I homolog CD1d are recognized by natural killer T cells ( NKT cells ) characterized by either a semi - invariant T cell antigen receptor ( TCR ) repertoire ( type I NKT cells or iNKT cells ) or a relatively variable TCR repertoire ( type II NKT cells ) . - pubmed
Here we describe the structure of a type II NKT cell TCR in complex with CD1d - lysosulfatide . - pubmed
Both TCR alpha - chains and TCR beta - chains made contact with the CD1d molecule with a diagonal footprint , typical of MHC - TCR interactions , whereas the antigen was recognized exclusively with a single TCR chain , similar to the iNKT cell TCR . - pubmed
Each chart was also reviewed for evidence of treatment with any cholinesterase inhibitor , memantine , or both . - pubmed
This study investigated optimizing the formulation parameters for encapsulation of a model mucinolytic enzyme , alpha - chymotrypsin ( alpha - CH ) , within a novel polymer ; poly ( ethylene glycol )- co - poly ( glycerol adipate - co - omega - pentadecalactone ) , PEG - co -( PGA - co - PDL ) which were then applied to the formulation of DNase I. alpha - CH or DNase I loaded microparticles were prepared via spray drying from double emulsion ( w(1)/o / w(2 ) ) utilizing chloroform ( CHF ) as the organic solvent , L - leucine as a dispersibility enhancer and an internal aqueous phase ( w(1 ) ) containing PEG4500 or Pluronic ( ) F-68 ( PLF68 ) . - pubmed
alpha - CH released from microparticles was investigated for bioactivity using the azocasein assay and the mucinolytic activity was assessed utilizing the degradation of mucin suspension assay . - pubmed
Optimum formulation alpha - CH - CHF / PLF68 , 1 % produced the highest bioactivity , enzyme encapsulation ( 20.08 + -3.91 % ) , loading ( 22.31 + -4.34 mug / mg ) , FPF ( fine particle fraction ) ( 37.63 + -0.97 % ) ; FPD ( fine particle dose ) ( 179.88 + -9.43 mug ) , MMAD ( mass median aerodynamic diameter ) ( 2.95 + -1.61 mum ) , and the mucinolytic activity was equal to the native non - encapsulated enzyme up to 5h . - pubmed
DNase I - CHF / PLF68 , 1 % resulted in enzyme encapsulation ( 17.44 + -3.11 % ) , loading ( 19.31 + -3.27 mug / mg ) and activity ( 81.9 + -2.7 % ) . - pubmed
Childhood immune thrombocytopenia : role of rituximab , recombinant thrombopoietin , and other new therapeutics . - pubmed
However , because immunosuppressive agents such as rituximab are not approved for use in ITP and the thrombopoietin receptor agonists are not yet approved in children , the decision to use alternatives to splenectomy needs to be considered carefully . - pubmed
Since externalization of phosphatidylserine ( PS ) is one of the early molecular events during apoptosis , Annexin B1 ( AnxB1 ) , a member of Annexins family with high affinity toward the head group of PS , could be a potential positron emission tomography ( PET ) imaging probe for imaging cell death process after labeled by positron - emitting nuclides , such as ( 18 ) F. - pubmed
In the present study , we investigated a novel PET probe , ( 18 ) F - labeled Annexin B1 ( ( 18)F - AnxB1 ) , for apoptosis imaging . - pubmed
( 18 ) F - AnxB1 was prepared reliably by conjugating AnxB1 with a ( 18 ) F - tag , N - succinimidyl 4 -[( 18 ) F ] fluorobenzoate ( [ ( 18 ) F ] SFB ) , in a radiolabeling yield of about 20 % within 40 min . - pubmed
The in vitro binding of ( 18 ) F - AnxB1 with apoptotic cells induced by anti - Fas antibody showed twofold increase compared to those without treatment , confirmed by flow cytometric analysis with AnxV - FITC / PI staining . - pubmed
( 18 ) F - AnxB1 PET / CT imaging was successfully applied to visualize in vivo apoptosis sites in tumor induced by chemotherapy and in kidney simulated by ischemia - reperfusion injury . - pubmed
As TNF - alpha plays a pivotal role in the induction of early atherosclerosis , treatment with TNF - inhibitors may modulate cholesterol handling , in particular , cholesterol efflux from macrophages . - pubmed
Two of these genes ( FAM129A , SYNPO2 ) were associated with airway hyperresponsiveness ( provocative concentration of methacholine causing a 20 % drop in FEV1 : r = -0.740 , P & lt ; 0.01 ; r = -0.746 , P & lt ; 0.01 ) . - pubmed
CONCLUSIONS : Oral prednisolone changes the transcriptomic profile of the ASM layer in asthma . - pubmed
Forward genetic studies have identified several chloride ( Cl- ) channel genes , including CFTR , ClC-2 , ClC-3 , CLCA , Bestrophin , and Ano1 , in the heart . - pubmed
A tetranuclear Cu4 ( mu3 - OH ) 2 - based metal - organic framework ( MOF ) with sulfonate - carboxylate ligands for proton conduction . - pubmed
A new tetranuclear Cu4 ( mu3 - OH ) 2 - based metal - organic framework ( MOF ) with sulfonate - carboxylate ligands features large hydrophilic channels . - pubmed
Nitric oxide in plants : the roles of ascorbate and hemoglobin . - pubmed
Ascorbic acid and hemoglobins have been linked to nitric oxide metabolism in plants . - pubmed
It has been hypothesized that ascorbic acid directly reduces plant hemoglobin in support of NO scavenging , producing nitrate and monodehydroascorbate . - pubmed
In this scenario , monodehydroascorbate reductase uses NADH to reduce monodehydroascorbate back to ascorbate to sustain the cycle . - pubmed
The product of the reaction is monodehydroascorbate , which can be efficiently reduced back to ascorbate in the presence of monodehydroascorbate reductase and NADH . - pubmed
However , our NO scavenging results suggest that the direct reduction of plant hemoglobin by ascorbic acid is unlikely to serve as a significant factor in NO metabolism , even in the presence of monodehydroascorbate reductase . - pubmed
Investigation of the mechanisms underlying the high acetylene - reducing activity exhibited by the soil bacterial community from BC2 horizon in the permafrost zone of the East Siberian larch forest bed . - pubmed
The calculated serum- ascites gradients for the cardiac and malignant disease groups had a greater frequency of negative values signifying greater levels of IL-8 , IL-10 and MCP-1 in ascites than did those with alcohol or HCV disease . - pubmed
BACKGROUND : Asymmetric dimethylarginine ( ADMA ) , the main endogenous inhibitor of nitric oxide synthase , is considered to be associated with endothelial dysfunction . - pubmed
Dual - time - point ( 18 ) F - FDG PET imaging ( whole body ) was performed at 1 - h ( early ) post - FDG injection and repeated ( 2 h delayed ) after injection . - pubmed
The diagnostic accuracy of pre - PET staging and post - PET staging was retrospectively evaluated , and the diagnostic accuracy of dual - time - point ( 18 ) F - FDG PET was compared to that of single - time - point ( 18 ) F - FDG PET . - pubmed
Sugar esters are non - ionic surfactants that can be synthesized in a single enzymatic reaction step using lipases . - pubmed
Automated blood glucose ( BG ) and insulin pump systems allow wireless transmission of all BG readings to a user 's pump . - pubmed
5,7,3 ' , 4 '- Tetramethoxyflavone exhibits chondroprotective activity by targeting beta - catenin signaling in vivo and in vitro . - pubmed
In addition , PGE2 increased the expression of cAMP / PKA signaling pathway in chondrocytes , stabilized and accumulated beta - catenin , and activated the expression of beta - catenin signaling pathway . - pubmed
Collectively , TMF is a potential compound with chondroprotective activity by inhibiting both EP / cAMP / PKA signaling pathway and beta - catenin signaling pathway . - pubmed
However , modeling and experimental studies on the intravenous anesthetic propofol indicate that potentiation of gamma - aminobutyric acid receptors leads to a state of thalamocortical synchrony , observed as coherent frontal alpha oscillations , associated with unconsciousness . - pubmed
Sevoflurane , an ether derivative , also potentiates gamma - aminobutyric acid receptors . - pubmed
The purpose of this study is to determine if there are differences in the expression of estrogen - regulated genes ( ERGs ) , proliferation - associated genes and the progesterone effector RANKL , in premenopausal ER + breast cancer as a result of the major changes in hormone levels that occur through the menstrual cycle . - pubmed
Expression of PGR , TFF1 , GREB1 and PDZK1 followed the previously reported pattern : a higher level in W2 compared to W1 while W3 had an intermediate value , mirroring changes in plasma estradiol . - pubmed
Cation exchange - based post - processing of ( 68 ) Ga - eluate : a comparison of three solvent systems for labelling of DOTATOC , NO2AP(BP ) and DATA(m . ) - pubmed
In this work three cation exchange resin based post - processing methods ( acetone , ethanol and NaCl ) have been compared , using three model precursors ( DOTATOC , NO2AP(BP ) and DATA ( m ) ) , in terms of labelling yield and reproducibility . - pubmed
PURPOSE : Preliminary iodine concentration ( UIC ) measurements in spot urines of the representative German adult study DEGS indicated a severe worsening of iodine status compared to previous results in German children ( KiGGS study ) . - pubmed
METHODS : UIC and creatinine concentrations were measured in 6978 spot urines from the German nationwide DEGS study ( 2008 - 2011 ) . - pubmed
RESULTS : In DEGS , median UIC was 69 microg / L in men and 54 microg / L in women , lying clearly below the WHO cutoff for iodine sufficiency ( 100 microg / L ) . - pubmed
Estimated median 24 - h UIE was 113 microg / day , accompanied by 32 % of DEGS adults , lying below the estimated average requirement ( EAR ) for iodine . - pubmed
Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA. - pubmed
In this work by mass spectrometry , we found that in the absence of nitrate , when NirA is inactive and predominantly cytosolic , methionine 169 in the nuclear export sequence ( NES ) is oxidized to methionine sulfoxide ( Metox169 ) . - pubmed
This oxidation depends on FmoB , a flavin - containing monooxygenase which in vitro uses methionine and cysteine , but not glutathione , as oxidation substrates . - pubmed
nidulans cells to nitrate led to rapid reduction of NirA - Metox169 to Met169 ; this reduction being independent from thioredoxin and classical methionine sulfoxide reductases . - pubmed
Replacement of Met169 by isoleucine , a sterically similar but not oxidizable residue , led to partial loss of NirA activity and insensitivity to FmoB - mediated nuclear export . - pubmed
Co - immunoprecipitation analysis of NirA - KapK interactions and subcellular localization studies of NirA mutants lacking different parts of the protein provided evidence that Met169 oxidation leads to a change in NirA conformation . - pubmed
Based on these results we propose that in the presence of nitrate the activation domain is exposed , but the NES is masked by a central portion of the protein ( termed nitrate responsive domain , NiRD ) , thus restricting active NirA molecules to the nucleus . - pubmed
In the absence of nitrate , Met169 in the NES is oxidized by an FmoB - dependent process leading to loss of protection by the NiRD , NES exposure , and relocation of the inactive NirA to the cytosol . - pubmed
To avoid potential confounders , LTRs on oral hypoglycemic agents , exogenous insulin , corticosteroids , or lipid - lowering therapy were excluded . - pubmed
LTRs with hepatic steatosis had higher serum concentrations of small - dense low - density lipoprotein cholesterol ( sdLDL - C ; 34.8 + - 16.9 versus 22.7 + - 11.2 mg / dL ; P & lt ; 0.001 ) , sdLDL - C to low - density lipoprotein cholesterol ratio ( 32.6 + - 11.6 versus 24.6 + - 10.2 ; P & lt ; 0.01 ) , small - dense low - density lipoprotein particle concentration ( sdLDL - P ; 770 + - 440 versus 486 + - 402 nmol / L ; P & lt ; 0.01 ) , very low density lipoprotein particle concentration ( VLDL - P ; 7.90 + - 7.91 versus 3.86 + - 3.18 nmol / L ; P & lt ; 0.01 ) , and very low density lipoprotein size ( VLDL - size ; 51.9 + - 6.4 versus 48.7 + - 6.3 nm ; P = 0.06 ) . - pubmed
LTRs with hepatic steatosis had higher serum insulin concentrations ( 27.8 + - 41.8 versus 11.7 + - 7.8 uU / mL ; P & lt ; 0.01 ) but similar fasting glucose and hemoglobin A1c . - pubmed
An increased amount of potassium significantly increases capacity , cycle life , and round - trip efficiency . - pubmed
The average effluent total nitrogen concentration was below 5 mg L(-1 ) and TN removal efficiency reached up to 97 % at COD / NO3 (- ) ratio of 3.0 under initial NH4 (+ ) concentration of 25 mg L(-1 ) and NO3 (- ) of 30 mg L(-1 ) . - pubmed
At Cornell , no differences emerged on improvement on the primary outcome , estimated speed of change over time on the PDSS ; at Penn , ART ( P = .025 ) and CBT ( P = .009 ) showed greater improvement at treatment termination than PFPP . - pubmed
Combined PCD / chlorine treatments resulted in greater disinfection efficiency than those for the two individual treatments . - pubmed
Combination of 0.9 MPa PCD ( various CO2 supply rates : 25 % CO2 + 75 % N2 , 50 % CO2 + 50 % N2 , and 100 % CO2 ) and chlorine ( 0.20 mg L-1 ) yielded average synergy values of 4.9 , 5.2 , and 4.4 log , respectively , within 3 min . - pubmed
This was deduced from the significant increase in H2O2 level , superoxide dismutase and catalase activities and malondialdehyde content . - pubmed
Maghemite ( gamma - Fe2O3 ) nanoparticles were obtained by coprecipitation of ferrous and ferric salts in an alkaline medium followed by oxidation ; the nanoparticles were coated with poly ( N , N - dimethylacrylamide ) ( PDMA ) and characterized by transmission electron microscopy , attenuated total reflection ( ATR ) Fourier transform infrared ( FTIR ) spectroscopy , dynamic light scattering , thermogravimetric and elemental analyses , and magnetic measurements in terms of particle morphology , size , polydispersity , amount of coating , and magnetization , respectively . - pubmed
Moreover , increased antitumor activity was observed after combined administration of gamma - Fe2O3@PDMA nanoparticles and Toc-6 - Ac ; however , levels of bilirubin , aspartate aminotransferase , and white bloods normalized and did not differ from those of the intact controls . - pubmed
This effect was associated to a significant inhibition of nitroglycerin - induced increase in c - Fos , TRPA1 and neuropeptides mRNA levels in medulla - pons area , in the cervical spinal cord and in the trigeminal ganglion . - pubmed
caroliniana were gradually compromised as time went by , and the down - regulations of the relative growth rate ( RGR ) and photosynthetic activity were coupled with phytoplankton proliferation under elevated atmospheric CO2 . - pubmed
L - mimosine induces caspase-9 - mediated apoptosis in human osteosarcoma cells . - pubmed
The Hoechst staining results and TEM revealed that nuclear damage increased with the concentration increase in L - mimosine , as did the formation of apoptotic bodies . - pubmed
Additionally , the results of the western blot analysis confirmed that the treatment of cells with L - mimosine was accompanied by increasing expression of cleaved caspase-9 . - pubmed
L - mimosine - induced apoptosis was inhibited by the caspase-9 inhibitor Z - LEHD - FMK . - pubmed
In addition , the extracellular signal - regulated kinase ( ERK ) signaling pathway was suppressed following treatment with L - mimosine . - pubmed
A total of 34 phenolic compounds were identified and quantitatively monitored by UPLC - ESI - QTOF - MS . - pubmed
This undesired triethanolamine deposit was easily removed from the surface by electrochemical voltage cycling or with an aqueous acidic wash for 1 h. - pubmed
Antiparasitic activity of new gibbilimbol analogues and SAR analysis through efficiency and statistical methods . - pubmed
The conversion of ent - CPP , ent - LPP , syn - CPP , and ent - neo - CT - CLPP by promiscuous diTPSs further yielded distinct labdane - type diterpene olefins and alcohols . - pubmed
Leptin increased carotid sinus nerve activity at baseline and in response to hypoxia in vivo . - pubmed
We found that leptin enhanced carotid sinus nerve activity at baseline and in response to 10 % O2 in vivo . - pubmed
Furthermore , TP42/40 was inversely associated with neocortical amyloid deposition ( measured with PiB - PET ) and was concordant with the AD biomarker profile in cerebrospinal fluid ( CSF ) . - pubmed
The results showed that 48 - h exposure to PS ( 0.1 mg / L ) or ROX ( 0.01 mg / L ) alone activated the activities of CAT and GST and MDA levels . - pubmed
magna co - exposed to 1 - mum PS were significantly decreased , while co - exposure to 10 - mum PS significantly decreased the responses of GST and MDA . - pubmed
Novel Isoxazole Derivatives with Potent FXR Agonistic Activity Prevent Acetaminophen - Induced Liver Injury . - pubmed
In this Letter , we have elaborated on the structure of GW4064 , the first nonsteroidal agonist for FXR , to identify novel isoxazoles endowed with FXR agonistic activity and improved ADME properties . - pubmed
While widely used fluorescent proteins , such as green fluorescent protein ( GFP ) , have had huge impact in biological imaging because they provide genetically encoded , highly specific labeling , these probes require oxygen to generate fluorescence . - pubmed
Luteolin supplementation also significantly lowered mRNA expression of inflammatory and M1 markers MCP-1 , CD11c , TNF - alpha and IL-6 , while maintaining expression of M2 marker MGL1 . - pubmed
pylori infection includes clarithromycin triple therapy ( clarithromycin , proton pump inhibitor [ PPI ] , and amoxicillin or metronidazole ) , bismuth quadruple therapy ( bismuth salt , PPI , tetracycline , and metronidazole or amoxicillin ) , or concomitant therapy ( clarithromycin , PPI , amoxicillin , and metronidazole ) . - pubmed
On d11 , P and NP were similar with low PGFM and no effect of oxytocin on PGFM . - pubmed
Alkynyl Benzoxazines and Dihydroquinazolines as Cysteine Targeting Covalent Warheads and Their Application in Identification of Selective Irreversible Kinase Inhibitors . - pubmed
A potent covalent inhibitor of JAK3 kinase was identified with superior selectivity across the kinome and improvements in in vitro pharmacokinetic profile relative to the related acrylamide - based inhibitor . - pubmed
In addition , the use of a novel heterocycle as cysteine reactive warhead is employed to target Cys788 in c - KIT where acrylamide has previously failed to form covalent interactions . - pubmed
Univariate analysis and multivariate Cox regression modelling were used to identify factors pre- and post - TACE therapy significantly associated with survival . - pubmed
On multivariate analysis greater tumour number ( p = 0.02 ) , higher serum bilirubin ( p = 0.007 ) post initial TACE , and hepatic decompensation ( p = 0.001 ) post second TACE were associated with reduced survival . - pubmed
On a per - lesion basis , the sensitivity of CEUS , MDCT , MRI , and FDG PET / CT was 86 % , 84 % , 89 % , and 62 % , respectively . - pubmed
On a per - patient basis , the sensitivity and specificity of CEUS , MDCT , MRI , and FDG PET / CT was 80 % and 97 % , 87 % and 95 % , 87 % and 94 % , and 96 % and 97 % , respectively . - pubmed
The sensitivity for MRI was higher than that for CEUS , MDCT , and FDG PET / CT for lesions < 10 mm and lesions at least 10 mm in size . - pubmed
Melatonin is a hormone that produces behavioral , pharmacological , and physiological effects through the activation of MT1 and MT2 melatonin receptors . - pubmed
Melatonin receptors participate in the modulation of the reinforcing effects of cocaine . - pubmed
Some studies report that dosing of melatonin decreases cocaine - induced locomotor activity and cocaine self - administration and that luzindole , an MT1 , and MT2 melatonin receptor antagonist , blocks the melatonin - dependent decrease in cocaine - induced locomotor activity . - pubmed
Melatonin decreased cocaine - induced locomotor sensitization and intracellular dopamine levels , as well as cocaine - CPP . - pubmed
These data suggest that melatonin may be a useful therapeutic agent to reduce cocaine abuse ; additionally , they suggest that MT1 and MT2 receptors could be therapeutic targets , useful for the treatment of drug abuse disorder . - pubmed
Besides , we isolated the promoter / enhancer of Ds serendipity alpha to drive the heterologous tetracycline - controlled transactivator ( tTA ) during early embryonic development and generated a testes - specific tTA driver line using the endogenous beta-2 - tubulin ( beta2 t ) promoter / enhancer . - pubmed
The experiment was tested on uniformly 15N- and 13C - enriched T4 lysozyme . - pubmed
The ability to react with fibronectin of the antigens of 58 - 60 and 30 - 31 kD was demonstrated by parallel labelling with a fibronectin - biotin conjugate . - pubmed
There currently exist various opinions concerning the best therapy for managing hydrogen fluoride ( HF ) dermal burns . - pubmed
Whole cell voltage - clamp recordings of the synaptic currents corresponding to these synaptic potentials in the presence of pharmacological antagonists of the neurotransmitters gamma - aminobutyric acid ( GABA ) , glutamate , and glycine receptor subtypes indicate that the inhibitory synaptic currents are mediated by GABA - activated Cl- channels , while the excitatory synaptic currents are due to activation of ionotropic glutamate receptors of the N - methyl - D - aspartic acid ( NMDA ) and non - NMDA subtypes . - pubmed
Phase I - II trial of hyperthermic isolated limb perfusion with cisplatin in the treatment of high risk malignant melanoma of the extremities . - pubmed
Concomitantly , activation of right atrial adenylate cyclase by 10 microM GTP , 10 microM isoprenaline and 1 microM forskolin was enhanced and inhibition by 100 microM carbachol was diminished . - pubmed
On isolated , electrically driven right atria the beta 1 - adrenoceptor - mediated positive inotropic effect of noradrenaline was - even with beta 1 - adrenoceptor number increased - not altered , while the beta 2 - adrenoceptor - mediated effect of procaterol was markedly enhanced . - pubmed
The effect of T4 non - carrying thyroxine binding globulin ( TBG ) on free thyroxine determination using the microencapsulated antibody method was studied , to investigate the precise reliability of the membrane and to find possible applications for estimating other free steroid hormones . - pubmed
All ( 6/6 ) FP / FL recipients were cured ( glucose less than 250 mg / dl for greater than 30 days ) , whereas only 72 % ( 13/18 ) of FP alone and 60 % ( 3/5 ) of FP / FS recipients were cured . - pubmed
In conclusion , FL in a composite graft with FP resulted in better engraftment , earlier isograft function , and protection from cyclosporine islet toxicity . - pubmed
When we treated the monolayers with drugs commonly used for the treatment of inflammatory bowel disease , including prednisolone , 5 - aminosalicylic acid , metronidazole , and 6 - mercaptopurine , we were not able to demonstrate a reduction in carrageenan - induced cell injury ; however , catalase at 1 mg / ml decreased carrageenan cytotoxicity . - pubmed
Phencyclidine binds with high affinity to both PCP and sigma receptors . - pubmed
Among the thirty - three nitro compounds other than the nitrofuran derivatives tested as substrates , the purified enzyme showed activity only toward o - dinitrobenzene , 4 - nitroquinoline N - oxide , 3,4 - dinitrobenzoic acid , p - dinitrobenzene , 2,5 - dinitrobenzoic acid , 2,5 - dinitrophenol , tetra - chloronitrobenzene and 2,4 - dinitrobenzoic acid . - pubmed
Among the other nine 2 - substituted 5 - nitrofuran derivatives tested , six compounds could be the substrates of the enzyme , and 5 - nitrofurfural and 5 - nitrofurfural diacetal were especially good substrates . - pubmed
The bulky substituents at position 2 appeared to decrease the susceptibility of these nitrofuran derivatives to the enzyme . - pubmed
In a short term study with 200 micrograms SMS bid , symptoms of hypercorticism disappeared while cortisol and ACTH serum concentrations fell below the normal values . - pubmed
Excellent clinical efficacy as well as normal cortisol and ACTH serum concentrations were maintained during the nine month follow up . - pubmed
These findings suggest that the contractile response to nicotine was mediated through an action on the nicotinic receptors and partially due to the release of acetylcholine and tachykinins . - pubmed
These findings suggest that chronic nicotine treatment might cause a decrease of the amounts of nicotinic receptors and also receptors for mediators released by nicotine . - pubmed
Chronic haloperidol treatment resulted in a 3.5 fold increase in striatal proenkephalin mRNA and a 30 % decrease in protachykinin mRNA ( no apparent change in alpha - tubulin mRNA was observed ) . - pubmed
The enzyme activity was stimulated 7 - fold in the presence of cardiolipin or phosphatidylglycerol ( and 3 - fold by phosphatidylethanolamine ) but not other phospholipids . - pubmed
The kinetic analysis indicated that addition of phosphatidylglycerol has a negligible effect on apparent Km values but increases the Vmax of the enzyme 5- to 6 - fold . - pubmed
The enzyme activity was solubilized by the dialyzable detergent CHAPSO without inhibition of the enzyme activity , and the solubilized enzyme in the presence of 0.4 % CHAPSO is partially purified by chromatography on GDP - hexanolamine - Sepharose . - pubmed
Removal of CHAPSO from the affinity purified enzyme by dialysis resulted in a 66 % loss of the original activity , which was restored by addition of phosphatidylglycerol . - pubmed
Chromatography of the affinity - purified enzyme with 3H - labeled phosphatidylglycerol on a Biogel A0.5 column indicated an association of the enzyme with the phospholipid that occurred only in the absence of detergent . - pubmed
These results suggest that phospholipid has a direct effect on the enzyme and that the inhibitory effect of detergents can be ascribable to disturbing interaction between phospholipids and the enzyme . - pubmed
A possible role of specific phospholipids on in vivo transferase activity for glycolipids is discussed . - pubmed
The results show that plasma and intraerythrocyte lactate concentrations were not significantly different from each other at rest or moderate ( less than or equal to 50 % VO2max ) exercise . - pubmed
The plasma to red cell lactate gradient reached a mean ( + /- SE ) 1.7 + /- 0.4 mmol . - pubmed
Interestingly , at 15 - min post - exercise the direction of the lactate gradient was reversed , with the mean intraerythrocyte concentration now being significantly increased over that found in the plasma . - pubmed
The best results were found in the males group , where cholesterol HDL increased and triglycerides and beta / alpha ratio decreased . - pubmed
In the females the only good result was represented by the increase of HDL - cholesterol , while the other values did not change . - pubmed
Chemical modification of cytochrome P-450 LM2 with N - acetylimidazole . - pubmed
Cytochrome P-450 LM2 has been reacted with N - acetylimidazole . - pubmed
About three tyrosyl residues of cytochrome P-450 LM2 are accessible to O - acetylation . - pubmed
The analysis of the spectral dissociation constants , substrate binding kinetics , reduction kinetics , N - demethylase activity and substrate conversion by artificial oxygen - donating agents of differently acetylated enzyme provides evidence for the existence of two groups of accessible tyrosines . - pubmed
One tyrosyl residue is located in the immediate environment of the heme iron and is involved in the binding of type II substrates . - pubmed
The results strongly suggest tyrosine(s ) to be involved in NADPH - cytochrome P-450 reductase dependent N - demethylation . - pubmed
The tubulin synthesized in vitro is characterized by ( i ) its electrophoretic mobility on sodium dodecyl sulfate - acrylamide gels ( brain : 55,000 daltons ; leg muscle : 53,000 daltons ) , and ( ii ) its ability to function as a microtubular subunit , as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro . - pubmed
Mechanism of urocanase as studied by deuterium isotope effects and labeling patterns . - pubmed
Most of the protein which bound to R 1881 was recovered in the precipitate of a 0 - 30 % saturation of ammonium sulfate , and was eluted in the void volume on a Sephadex G-200 column . - pubmed
The renin - angiotensin - aldosterone system during and after cardiopulmonary bypass . - pubmed
A chromatographic system consisting of Sorbax - SIL and Sorbax - CN columns , organic solvent extraction and a UV detector was used to analyze both more polar and less polar corticosteroids in the adrenal tissue . - pubmed
In addition , competition with the radioactively labeled translation product occurred only in the presence of purified Vt , but not BSA or LPI . - pubmed
The removal of oligosaccharides from purified Vt by enzymatic digestion with N - glycosidase F resulted in the appearance of a smaller polypeptide of 36 kDa , which reacted immunologically with Vt antiserum in a Western blot . - pubmed
After 6 - OHDA lesion , as well as caudally to the transection , a significant increase by 21 % ( P & lt ; 0.01 ) to 68 % ( P & lt ; 0.001 ) of alpha 1 - adrenoceptors densities was detected . - pubmed
The mechanism of action seems to be through decreasing intracellular calcium by inhibiting its uptake and stimulating the efflux , and by an inhibition on protein kinase C. - pubmed
Extracellular glutamate rose quickly after vessel occlusion and peaked at 33.95 + /- 6.3 mumol / ml 30 minutes after MCAO . - pubmed
By 60 minutes after MCAO , this level had decreased to 25.14 + /- 6.3 mumol / ml ; glutamate levels decreased slightly to 21.35 + /- 6.8 mumol / ml by 120 minutes . - pubmed
Long - term treatment with the cholinesterase inhibitor tacrine increase the uptake of [ 11C ] nicotine . - pubmed
Significant reduction in uptake between the two enantiomers ( S)(- ) and ( R )(+)-[ 11C ] nicotine has been observed compatible with a restoration of nicotinic receptors . - pubmed
A point mutation in the bile acid biosynthetic enzyme sterol 27 - hydroxylase in a family with cerebrotendinous xanthomatosis . - pubmed
An increase in plasma cholestanol alone would , thus , not appear to be a direct cause of sterol storage in CTX , while CTX is strongly suggested to be caused by defects in both alleles of the CYP27 gene . - pubmed
These results suggest that the fast inactivating ( transient ) current might be T - type Ca2 + current [ ICa(T ) ] , and such cells were ICa(T ) positive cells ; whereas the sustained Ca2 + current was L - type Ca2 + current [ ICa(L ) ] , and such cells were ICa ( L ) positive cells . - pubmed
Kinetic and pH titration experiments reveal that when His57 is deprotonated the enzyme binds its substrates randomly . - pubmed
However , when His57 is protonated the enzyme observes the obligatory substrate binding order as seen for the wild type . - pubmed
A comparison of the reaction schemes and pH profiles of the wildtype and His57 enzymes further indicates that protonation of residue 57 facilitates formation of the binary enzyme - carbamoyl phosphate complex and augments the turnover rate of the reaction . - pubmed
265 , 2620 - 2623 ) that the turnover rate of the wild - type ornithine transcarbamoylase in the forward reaction is largely dictated by the rate of the carbamoyl phosphate - induced isomerization . - pubmed
Highest levels of serum cholesterol were found in spring and lowest in summer . - pubmed
These changes were measured using MTT to assess mitochondrial reductase activity. - pubmed
Phosphatidylinositol-4 - phosphate 5 - kinases ( PIP5 K ) synthesize phosphatidylinositol-4,5 - bisphosphate , a key precursor in phosphoinositide signaling that also regulates some proteins and cellular processes directly . - pubmed
Thus , CD22 negatively regulates antigen receptor signaling in the absence of antigen . - pubmed
Mechanisms of centrally administered ET-1 - induced increases in systemic arterial pressure and AVP secretion . - pubmed
Endothelins ( ET ) within the central nervous system ( CNS ) alter systemic cardiovascular responses and arginine vasopressin ( AVP ) secretion . - pubmed
These experiments were designed to ascertain whether the rise in systemic arterial pressure after central administration of ET-1 is mediated by enhancing sympathetic outflow and/or circulating AVP . - pubmed
Thus ET-1 via ETA receptors within the CNS induced a concentration - dependent increase in systemic arterial pressure mediated by enhanced sympathetic outflow but not by circulating AVP . - pubmed
While whole body glucose removal and production may be reduced following training , this has only been demonstrated under exercising conditions in which glycemia demonstrates little deviation from rest . - pubmed
The CR1 genotype was determined by a method based on polymerase chain reaction ( PCR ) amplification of the genomic DNA fragment of 1.8 kb separated by HindIII endonuclease digestion and agarose gel electrophoresis . - pubmed
Beta - lactam resistance in mycobacteria results from an interplay between the following : ( i ) beta - lactamase production , ( ii ) affinity of the penicillin - binding proteins ( PBPs ) for the drugs , and ( iii ) permeation of the drugs . - pubmed
However , the affinities of PBP 1 for the beta - lactams tested were lower for the mutant than for the wild type . - pubmed
Taken together , the results suggest that decreased affinities of PBP 1 for beta - lactams , combined with the decreased permeability of the cell wall of the mutant , lead to the development of high - level acquired beta - lactam resistance . - pubmed
This effect , which was also observed with amphotericin B - treated corneas , was not mediated by classical beta - receptors based on the results obtained with isoproterenol and propranolol . - pubmed
The similarities , of a sequence motif found in potassium channels and beta - adrenergic receptor kinases that are regulated by the beta gamma subunit of G proteins with that found in aquaporins 2 and 5 , could explain the link with epinephrine . - pubmed
or administered orally led to a marked loss of uterine and vaginal estrogen receptor levels measured by binding assay , whereas ICI 182 780 exerted no inhibitory effect on this parameter under the experimental conditions used . - pubmed
Similarly , because P450s are catalyzing the toxication and detoxication of environmental pollutants , an understanding of these reactions facilitates risk assessment in environmental toxicology . - pubmed
RESULTS : Using multiple linear regression analysis , total calcium scores were better independent predictors of both CAGE & gt ; or = 20 and CAGE & gt ; or = 50 scores than either a SPECT - derived radionuclide perfusion score or the risk factors age , male gender and ratio of total / high - density lipoprotein ( HDL ) cholesterol . - pubmed
Biochemical analyses revealed that Abeta purified from senile plaques and vascular amyloid are isomerized at Asp-1 and Asp-7 . - pubmed
A specific antibody recognizing isoAsp-23 of Abeta further suggested the isomerization of Abeta at Asp-23 in vascular amyloid as well as in the core of senile plaques . - pubmed
Biochemical analyses of purified PHFs also revealed that heterogeneous molecular weight tau contains L - isoaspartate at Asp-193 , Asn-381 , and Asp-387 , indicating a modification , other than phosphorylation , that differentiates between normal tau and PHF tau . - pubmed
Protein L - isoaspartyl methyltransferase ( PIMT ) is suggested to play a role in the repair of isomerized proteins containing L - isoaspartate . - pubmed
By multivariate analysis , the DH ( + ) and DH ( - ) groups of NTG showed significantly longer peak times and tau s than did the non - glaucomatous eyes ( p & lt ; 0.05 ) . - pubmed
However , no statistically significant differences were observed in any parameters between the DH ( + ) and DH ( - ) groups of NTG . - pubmed
A new covalent modification of p53 at lysine 386 by SUMO-1 was recently identified . - pubmed
These data indicate that SUMO-1 modification of p53 at lysine 386 may not be essential for p53 's cellular localization , transcriptional activation , or growth regulation . - pubmed
In the C480Y mutant , tyrosine 480 protrudes from S6 to near S5 , suggesting that the bulky side chain may provide steric hindrance to the rotation of S6 that has been proposed to accompany the open - closed state transitions ( Perozo , E. , D.M. - pubmed
The side chain at position 480 in S6 in the pore model is close to and may interact with a conserved glycine ( G421 ) in S5 . - pubmed
Replacement of G421 with bulky side chains also leads to channels that are trapped in an active state , suggesting that S5 and S6 interact with each other during voltage - dependent open - closed state transitions , and that bulky side chains prevent the dynamic changes necessary for permanent channel closing . - pubmed
The specific activity of lipoxygenase from several strains of the zygomycete Mortierella varied from 1.02 to 2.02 microMol diene per min per mg protein ) . - pubmed
The enzyme equally used linoleic or arachidonic acid as a substrate . - pubmed
NDGA inhibited the enzyme in vitro ( IC50 = 142 microM ) , but its addition in the exponential phase of growth activated the enzyme . - pubmed
The substrates for measuring the activity of individual CYP in vitro included phenacetin , tolbutamide , debrisoquine , and omeprazole . - pubmed
Both plant species were assessed for sticky chromosomes by Feulgen - stained mitotic analysis and flow cytometric analysis of propidium iodide ( PI)-stained G1 nuclei . - pubmed
The neurotransmitter L - glutamate also led to activation of NF - kappaB and electrophysiology recordings showed a small but sustained increase in synaptic transmission ( fEPSP 106+/-12 % , 30 min post - drug ) . - pubmed
After a median of 48 ( range 2 - 126 ) weeks of therapy ( IFN , n=190 ; IFN and ribavirin , n=96 ; pegylated IFN , n=20 ; pegylated IFN and ribavirin , n=26 ) , 93 patients ( 28 % ) achieved an SVR . - pubmed
These studies demonstrate that inhibitors of PP1 can be used in a tissue- and developmental - specific manner to examine the developmental roles of PP1 . - pubmed
Anti - thrombin , anti - platelet and anti - vitamin K agents are common treatments given for the prevention of thrombotic or embolic events . - pubmed
Thiopental inhibits tumor necrosis factor alpha - induced activation of nuclear factor kappaB through suppression of kappaB kinase activity . - pubmed
The authors have recently reported that thiopental inhibits activation of nuclear factor ( NF ) kappaB , a transcription factor implicated in the expression of many inflammatory genes . - pubmed
METHODS : The authors tested gamma - aminobutyric acid ( GABA ) , the GABA(A ) antagonist bicuculline , and the GABA(B ) antagonist dichlorophenyl - methyl - amino - propyl - diethoxymethyl - phosphinic acid ( CGP 52432 ) in combination with thiopental for their influence on the activation of NF - kappaB. - pubmed
In addition , they investigated the direct effect of thiopental on activated NF - kappaB DNA binding activity . - pubmed
In addition , the authors tested the effect of the structural barbiturate analog pairs thiopental - pentobarbital and thiamylal - secobarbital and of thiopental in combination with the thio - group containing chemical dithiothreitol on the activation of NF - kappaB. - pubmed
RESULTS : GABA did not inhibit NF - kappaB activation , and the GABA(A ) and GABA(B ) antagonists bicuculline and CGP did not diminish the thiopental - mediated inhibitory effect on NF - kappaB activation . - pubmed
Thiopental did not inhibit activated NF - kappaB directly in a cell - free system . - pubmed
The phosphorylation of IkappaBalpha was prevented after incubation with 1,000 microg / ml thiopental . - pubmed
Thiobarbiturates ( 4 x 10(-3 ) m ) inhibited NF - kappaB activity , whereas equimolar concentrations of the structural oxyanalogs did not . - pubmed
CONCLUSION : Thiopental - mediated inhibition of NF - kappaB activation is due to the suppression of IkappaB kinase activity and depends at least in part on the thio - group of the barbiturate molecule . - pubmed
Using standard immunocytochemical and light - microscopic techniques , the distribution of GABA - ergic neurons ( and their co - localized calcium - binding proteins : calbindin ( CB ) , calretinin ( CR ) and parvalbumin ( PV ) ) was determined for each area . - pubmed
These data confirmed that there were striking differences in the distribution of GABA- , CB- , CR- and PV - positive neurons . - pubmed
Because the chemical structure of the interface plays an important role in the coating adhesion , we studied the interface between CaP and the polymers polyethylene ( PE ) and polydimethylsiloxane ( PDMS / silicone rubber ) . - pubmed
On PE , a low Ca / P ratio nearby the interface and a high amount of C - O bonds were found on both untreated and plasma pretreated PE . - pubmed
Amylase , IgA , proline - rich proteins and the high - molecular - weight glycoproteins , agglutinins , were detected in all saliva tested . - pubmed
Carbohydrate - reactive antibodies recognized the low - molecular weight mucin , MUC 7 in submandibular / sublingual saliva only . - pubmed
HORTON ' S SYNDROME , A GENETIC DISEASE : The preferential association of the disease with some alleles pf the HLA DR4 group has helped to emphasize the fundamental role of a few amino acids of the second hypervariable area of the HLA - DR molecule . - pubmed
YB-1(+/- ) cells showed increased sensitivity to cisplatin and mitomycin C , but not to etoposide , X - ray or UV irradiation , as compared to YB-1(+/+ ) cells . - pubmed
These bacteriocins are characterized by the presence , in their primary structure , of post - translationally modified amino acid residues ( i.e. - pubmed
Class II bacteriocins , containing three subclasses , consist of small peptides that do not bear any modified amino acid residue . - pubmed
Moreover , some of these bacteriocins , namely , sakacin G , pediocin PA-1 , enterocin A , coagulin , and divercin V41 , are characterized by the presence of a second disulfide bond in the C - terminal region . - pubmed
RESULTS : As compared with vitamin E , three doses of PHAS ( 1.6 , 0.8 and 0.4 ml / d ) prolonged the survival time to hypoxia and the pole climbing time and increased the heat survival rate , and the 0.8 ml / d PHAS had the best effect . - pubmed
In the group of 0.8 ml / d PHAS , the activity of SOD and CAT decreased less , and the contents of lipofuscin and MDA decreased significantly . - pubmed
The key step involves the one - pot reaction for 2 - arylbenzofurans ( 2 , 7 ) from 4 - hydroxyphenylacetone with 4 '- acetoxy-2 - chloro-2 -( methylthio ) acetophenone ( 1 ) and 2 - chloro-2 - methylthio -( 2 ' , 4 ' , 6 '- trimethoxy ) acetophenone ( 6 ) under Friedel - Crafts reactionconditions . - pubmed
In vitro , inhibition of NTE activity by organophosphates is correlated with inhibition of neurite initiation and reduction of neurite length , supporting the hypothesis that organophosphate - induced neurological disorders are caused by inhibition of NTE activity . - pubmed
However , there is no direct evidence for the involvement of NTE in organophosphate - induced impairment of neurites in vitro . - pubmed
Liver X receptors ( LXRs ) are nuclear receptors that regulate macrophage cholesterol efflux by inducing ATP - binding cassette transporter A1 ( ABCA1 ) and ABCG1 / ABCG4 gene expression . - pubmed
The Niemann - Pick C ( NPC ) proteins NPC1 and NPC2 are located in the late endosome , where they control cholesterol trafficking to the plasma membrane . - pubmed
Synthetic LXR activators increase the amount of free cholesterol in the plasma membrane by inducing NPC1 and NPC2 gene expression . - pubmed
Moreover , ABCA1 - dependent cholesterol efflux induced by LXR activators was drastically decreased in the presence of progesterone , which blocks postlysosomal cholesterol trafficking , and reduced when NPC1 and NPC2 mRNA expression was depleted using small interfering RNA . - pubmed
The stimulation of cholesterol mobilization to the plasma membrane by LXRs led to a decrease in cholesteryl ester formation and Acyl - coenzyme A cholesterol acyltransferase-1 activity . - pubmed
These data indicate that LXR activation enhances cholesterol trafficking to the plasma membrane , where it becomes available for efflux , at the expense of esterification , thus contributing to the overall effects of LXR agonists in the control of macrophage cholesterol homeostasis . - pubmed
The minimal model ( MM ) of glucose kinetics during an intravenous glucose tolerance test ( IVGTT ) is widely used in clinical studies to measure metabolic indexes such as glucose effectiveness ( S(G ) ) and insulin sensitivity ( S(I ) ) . - pubmed
Although the risk was observed originally with trials conducted with rofecoxib , it was attributed generally to the entire class of COX-2 - selective drugs based on a similar mechanism of action and a hypothesis that predicted the possibility of a prothrombotic effect of the drugs compared with nonselective NSAIDs . - pubmed
In contrast , the genes for the controlling enzymes of heme synthesis and degradation ( 5 - aminolevulinate synthase 1 and heme oxygenase 1 , respectively ) were up - regulated , implying depletion of a regulatory heme pool . - pubmed
Inhibition of heme synthesis ( by 70 - 80 % ) at different enzymic steps by succinyl acetone and N - methylprotoporphyrin IX resulted in the earlier lowered expression of NMDAzeta1 and -epsilon2 and NF - L. - pubmed
A single cotyledon was localized , perfused and stabilized with physiologic Eagles minimal essential medium containing 3 % bovine albumin and heparin as described by Chalier ( Chalier JC . - pubmed
BNP as a marker of the heart failure in the treatment of imatinib mesylate . - pubmed
Since its introduction 6 years ago , imatinib mesylate , a selective tyrosine kinase inhibitor , has been a phenomenon in treating chronic myelogenous leukemia ( CML ) with remarkably superior cytogenetic and molecular response rates at all stages of CML followed by longer progression free survival . - pubmed
The selected clones express progesterone ( PR ) and estrogen receptor - alpha ( ER - alpha ) at both mRNA and protein levels . - pubmed
Two 16S rRNA gene libraries were constructed using the reverse transcription polymerase chain reaction ( RT - PCR ) method to determine the major ammonia oxidizers maintaining high cellular rRNA content . - pubmed
In addition to this competition for glyoxylate , competitive inhibition at the active site of enzymes occurred between glutamate and alanine , but not between serine and glutamate or alanine . - pubmed
Phosphodiesterases ( PDEs ) regulate cyclic nucleotide levels . - pubmed
Increased cyclic AMP ( cAMP ) signaling has been associated with PRKAR1A or GNAS mutations and leads to adrenocortical tumors and Cushing syndrome . - pubmed
Tumor tissues showed 2q31 - 2q35 LOH , decreased protein expression and high cyclic nucleotide levels and cAMP - responsive element binding protein ( CREB ) phosphorylation . - pubmed
PDE11A codes for a dual - specificity PDE that is expressed in adrenal cortex and is partially inhibited by tadalafil and other PDE inhibitors ; its germline inactivation is associated with adrenocortical hyperplasia , suggesting another means by which dysregulation of cAMP signaling causes endocrine tumors . - pubmed
The least - correlated parameters were SRAX3 , TOP magnitude , and II3 in the KCN group and I - S K3 , amount of astigmatism and MPE magnitude in the normal group . - pubmed
Effects of ERBB2 amplicon size and genomic alterations of chromosomes 1 , 3 , and 10 on patient response to trastuzumab in metastatic breast cancer . - pubmed
In 35 patients with ERBB2 - amplified metastatic breast cancer , with 40 % overall response to trastuzumab , fluorescence in situ hybridization identified the telomeric extent of the ERBB2 amplicon and the status of the several pathway - related genes . - pubmed
These results suggest that patient selection for trastuzumab may be improved by considering ERBB2 amplicon size and genomic status of the 1q25 , PTGS2 , and centromere 10 loci . - pubmed
Then , the solid complexes of genistein with these macrocycles in 1:1 molar ratio were prepared by the co - precipitation method and characterized by FTIR absorption spectroscopy in ATR geometry . - pubmed
The protein , designated Cancerous Inhibitor of PP2A ( CIP2A ) , interacts directly with the oncogenic transcription factor c - Myc , inhibits PP2A activity toward c - Myc serine 62 ( S62 ) , and thereby prevents c - Myc proteolytic degradation . - pubmed
Taken together , PF4 - stimulated immediate monocyte functions ( oxygen radical formation ) are regulated by p38 MAPK , Syk , and PI3 K , whereas delayed functions ( survival and cytokine expression ) are controlled by Erk and JNK . - pubmed
The reactions of a model sulfur ylide with formaldehyde and 1,1 - dicianoethylene , leading to the formation of an epoxyde and a cyclopropane , respectively , have been studied using different computational methods , and the results have been compared to those obtained with the CBS - QB3 method . - pubmed
Monomer formation and function of p - hydroxybenzoate hydroxylase in reverse micelles and in dimethylsulfoxide / water mixtures . - pubmed
Surprisingly , highly conserved consensus sites for binding post - synaptic density-95 / discs large / zonula occludens-1 - domain - containing ( PDZ ) proteins and serines with a high probability of phosphorylation play no significant role in myoblast fusion . - pubmed
Biochemical studies establish that the SNS cytodomain is phosphorylated at multiple tyrosines and their site - directed mutagenesis compromises the ability of the corresponding transgenes to rescue myoblast fusion . - pubmed
This complexity and redundancy of multiple critical sequences within the SNS cytodomain suggest that it functions through a complex array of interactions that likely includes both phosphotyrosine - binding and SH3 - domain - containing proteins . - pubmed
The clinical symptoms , serum levels of triglyceride ( TG ) and APACHE II scores were observed before and after DFPP . - pubmed
In the presence of pronase E , for example , glycoproteins are digested to small glycopeptides having one to six amino acids residues , which can be analyzed with excellent sensitivity using mass spectrometry . - pubmed
When digesting glycoproteins , pronase E produced glycopeptides within 5min under microwave irradiation ; glycopeptides having one or two amino acids were the major products . - pubmed
Although analysis of peptides containing multiple amino acid residues offers the opportunity for peptide sequencing and provides information regarding the sites of glycosylation , the signals of Asn - linked glycans were often suppressed by the glycopeptides containing basic amino acids ( Lys or Arg ) in MALDI - MS experiments . - pubmed
This feature can be used to elucidate glycan structures and glycosylation sites without the need for MS / MS analysis . - pubmed
After ipsilateral injections of biotinylated dextran amine ( BDA ) into the CeA and cholera toxin B subunit ( CTb ) into the NRA , the prominent overlapping distribution of BDA - labeled axon terminals and CTb - labeled neurons was found ipsilaterally in the lateral / ventrolateral PAG , where some of the BDA - labeled terminals made symmetrical synaptic contacts with somata and dendrites of the CTb - labeled neurons . - pubmed
After BDA injection into the lateral / ventrolateral PAG , BDA - labeled fibers were distributed mainly in and around the NRA within the medulla oblongata . - pubmed
CA was induced in vitro in a medium containing BSA , CaCl ( 2 ) , NaHCO ( 3 ) , and AR by the addition of the calcium ionophore A23187 . - pubmed
Flow cytometry ( FC ) analyses were performed by loading samples with Fluo-3 AM , that emits fluorescence at a high [ Ca ( 2 +)]( i ) , combined with propidium iodide ( PI ) that allowed us to discriminate sperm with / without an integral plasma membrane both with high / low [ Ca ( 2 +)]( i ) . - pubmed
The treatment of sperm with increasing concentrations of two CaM antagonists , W7 and calmidazolium ( CZ ) , accounted for an increase in capacitated and acrosome - reacted CTC - sperm patterns . - pubmed
After AR , only 7.2 % of the total sperm preserved intact membranes with a very high [ Ca(2+)](i ) . - pubmed
Elucidation of phototransformation reactions of the X - ray contrast medium iopromide under simulated solar radiation using UPLC - ESI - QqTOF - MS . - pubmed
Following a 120 - min irradiation period , an almost complete primary degradation of iopromide gave rise to a series of photoproducts that were chromatographed on a reversed - phase UPLC and subsequently characterized by a combination of accurate mass measurements on a ESI - QqToF - MS instrument and H / D - exchange experiments . - pubmed
Translational fusions of lacZ to each rsbR paralogue revealed that rsbRA , -RB and -RC are expressed at similar levels , which remain relatively constant during growth , ethanol stress and entry into stationary phase . - pubmed
rsbRD , in contrast , is expressed at a level that is only slightly above background during growth , but is induced to 30 % of the rsbRA expression level following ethanol stress . - pubmed
The evolutionary conserved sorting and assembly machinery ( SAM complex ) assembles mitochondrial beta - barrel proteins , such as voltage - dependent anion - selective channel 1 ( VDAC1 ) , into complexes in the outer membrane by recognizing a sorting beta - signal in the carboxy - terminal part of the protein . - pubmed
The length of the tag at the C - terminus is critical for the assembly of VDAC1 , as well as the amino acid residues at positions 130 , 222 , 225 and 251 of the protein . - pubmed
STAT3 inhibitor WP1066 as a novel therapeutic agent for renal cell carcinoma . - pubmed
We examined whether it could be a novel therapeutic target for RCC by using the STAT3 inhibitor WP1066 . - pubmed
WP1066 suppressed the expression of Bcl-2 , induced apoptosis , and inhibited the basal and hypoxia - induced expression of HIF1alpha and HIF2alpha , as well as vascular endothelial growth factor secretion into cell culture medium . - pubmed
CONCLUSION : Our results suggest that using WP1066 to inhibit the STAT3 signalling pathway could be a novel therapeutic strategy against RCC . - pubmed
Paclitaxel was administered at 120 mg / m(2 ) on day 1 and S-1 was administered orally at 80 mg / m(2 ) for 14 consecutive days , followed by a 1 - week rest , as one course . - pubmed
Expressions of biopyrrins and heme oxygenase-1 ( HO-1 ) , a stress - responsive bilirubin - producing enzyme , in heart , aorta , kidney , liver and lung were immunostained with autopsied specimens . - pubmed
Immunoreactive HO-1 and biopyrrins were also observed in renal tubular cells , aortic wall and lung . - pubmed
Induction of anti - oxidative enzyme HO-1 seemed to be involved in the activation of bilirubin / biopyrrin pathway . - pubmed
In contrast , the kinetics of c - myc and c - Ha - ras inducibility were enhanced by BaP ( 3 mum ) leading to significant enhancement of mRNA levels . - pubmed
Challenge with 2,3,7,8 - tetrachlorodibenzo - p - dioxin ( TCDD ) ( 1 nm ) , an aryl hydrocarbon receptor ( AhR ) agonist , elicited responses comparable to BaP for c - jun and c - Ha - ras , but not for c - fos or c - myc . - pubmed
alpha - naphthoflavone ( 10 nm ) , an antagonist / agonist of the AhR and inhibitor of cytochrome P450 , elicited c - jun and c - Ha - ras responses comparable to BaP. - pubmed
Pretreatment of the cells with this agent nullified the modulatory effects of BaP on c - fos and c - myc . - pubmed
Carbohydrate decoration of microporous polypropylene membranes for lectin affinity adsorption : comparison of mono - and disaccharides . - pubmed
As the BD of saccharide increases , the " glycoside cluster effect " plays a primary role in lectin adsorption . - pubmed
Bz-423 , a small - molecule inhibitor of the mitochondrial F ( 1 ) F ( 0 ) adenosine triphosphate synthase ( F ( 1 ) F ( 0 )- ATPase ) , selectively increased superoxide and induced the apoptosis of alloreactive T cells , which arrested established GVHD in several BMT models without affecting hematopoietic engraftment or lymphocyte reconstitution . - pubmed
Alpha - synuclein ( SNCA ) is associated with a range of psychiatric diseases including neurodegeneration , alcohol craving , and depression . - pubmed
BACKGROUND AND PURPOSE : The cystic fibrosis transmembrane conductance regulator ( CFTR ) is a cAMP - dependent chloride channel in the plasma membrane of epithelia whose mutation is the cause of the genetic disease cystic fibrosis ( CF ) . - pubmed
The most frequent CFTR mutation is deletion of Phe(508 ) and this mutant protein ( delF508CFTR ) does not readily translocate to the plasma membrane and is rapidly degraded within the cell . - pubmed
CONCLUSION AND IMPLICATIONS : These results show that resveratrol modulated CFTR expression and localization and could rescue cAMP - dependent chloride transport in delF508CFTR cells . - pubmed
PRINCIPAL FINDINGS : The paper summarizes the general findings including various classes of compounds , and more specific information on two veterinary anthelmintics ( monepantel , emodepside ) and nitazoxanide , an antiprotozoal drug , compiled from the EMA EPAR and FDA registration files . - pubmed
The levels of serum pituitary gonadotropin including estradiol , follicle - stimulating hormone , luteinizing hormone , prolactin and testosterone were measured before treatment and 3 months , 6 months and 1 year after treatment . - pubmed
Interface pressures ( IPs ) and transcutaneous blood gas levels [ transcutaneous tissue oxygen ( T(c ) PO(2 ) ) ] were simultaneously assessed over both ischia and the sacrum to investigate the hypotheses : ( i ) tissue oxygenation decreases with sustained applied pressure ; ( ii ) tissue oxygen and IP are inversely correlated in loaded soft tissues ; ( iii ) multisite assessments are unnecessary because healthy individuals are symmetrical . - pubmed
The medium chain triglyceride ( MCT ) ketogenic diet is used extensively for treating refractory childhood epilepsy . - pubmed
We identify specific medium chain fatty acids ( both prescribed in the MCT diet , and related compounds branched on the fourth carbon ) that provide significantly enhanced in vitro seizure control compared to VPA . - pubmed
The activity of these compounds on seizure control is independent of histone deacetylase inhibitory activity ( associated with the teratogenicity of VPA ) , and does not correlate with liver cell toxicity . - pubmed
Our data therefore implicates medium chain fatty acids in the mechanism of the MCT ketogenic diet , and highlights a related new family of compounds that are more potent than VPA in seizure control with a reduced potential for side effects . - pubmed
Adding 3 % Tween 20 or 1 % catalase enabled cells to become culturable again , with resuscitation times of 48 h and 24 h , respectively . - pubmed
Because UGT2B17 metabolizes certain nonsteroidal anti - inflammatory drugs and flavonoids with antioxidative properties , individuals with a gene deletion may have higher levels of these protective dietary components . - pubmed
Glucose is an energy source that also controls the expression of key genes involved in energetic metabolism through the glucose - signaling transcription factor carbohydrate response element - binding protein ( ChREBP ) . - pubmed
ChREBP has recently emerged as a central regulator of glycolysis and de novo fatty acid synthesis in liver , but new evidence shows that it plays a broader and crucial role in various processes , ranging from glucolipotoxicity to apoptosis and/or proliferation in specific cell types . - pubmed
However , several aspects of ChREBP activation by glucose metabolites are currently controversial , as well as the effects of activating or inhibiting ChREBP , on insulin sensitivity , which might depend on genetic , dietary or environmental factors . - pubmed
Crescentic glomerulonephritis due to IgA nephropathy or propylthiouracil - induced Mpo - Anca positive vasculitis ? - pubmed
Currently , the best - suited piezoelectric material for high - temperature SAW applications is langasite ( LGS ) . - pubmed
A gas chromatography ( GC ) method for the analysis of pymetrozine was developed after utilizing matrix enhancement effect of pymetrozine to nitrogen phosphorus detector ( NPD ) . - pubmed
Samples were extracted with acetonitrile and purified through primary secondary amine ( PSA ) and C18 dispersive sorbent . - pubmed
Estrogen insufficiency decreases defense against oxidative stress ; skin becomes thinner with less collagen , decreased elasticity , increased wrinkling , increased dryness and reduced vascularity . - pubmed
The relevance of estrogen replacement , selective estrogen receptor modulators ( SERMs ) and phytoestrogens as therapies for diminishing skin aging is highlighted . - pubmed
The datasets were derived using Illumina high - throughput sequencing and were based on BLAST against self - established databases of BDGs , DDT degradation genes ( DDGs ) , HCH degradation genes ( HDGs ) , and ATZ degradation genes ( ADGs ) . - pubmed
The hdt , hdg , and atzB genes , which encode for hydratase , dehalogenase , and ethylaminohydrolase , were found to be the most abundant genes involved in DDT , HCH , and ATZ degradation , respectively . - pubmed
Here , we examine oxygen - deficient H : TiO2 nanotube arrays that have previously achieved very high solar - to - hydrogen ( STH ) efficiencies due to incident photon - to - current efficiency ( IPCE ) values of & gt;90 % for photoelectrochemical water splitting at only 0.4 V vs RHE under UV illumination . - pubmed
Neuroglobin is a brain globin with neuroprotective effects against ischemia and related pathological processes , acting as O2 sensor and antiapoptotic pathway transducer . - pubmed
The one - pot reaction involving a Cu ( I )/ Pd ( 0 ) cooperative dual catalyst directly addresses the need to develop more time - efficient and less - wasteful synthetic pathways . - pubmed
Defining a therapeutic window for the novel TGF - beta inhibitor LY2157299 monohydrate based on a pharmacokinetic / pharmacodynamic model . - pubmed
AIMS : To identify prospectively a safe therapeutic window for administration of a novel oral transforming growth factor beta ( TGF - beta ) inhibitor , LY2157299 monohydrate , based on a pharmacokinetic / pharmacodynamic ( PK / PD ) model . - pubmed
Insulin is widely used to control posttrauma glucose , but this treatment increases the risk of hypoglycemia . - pubmed
We tested a novel method for early posttrauma hyperglycemia control by suppressing hepatic glycogenolysis via beta2 - adrenoreceptor blockade [ ICI-118551 ( ICI ) ] . - pubmed
AIM OF THE STUDY : End tidal carbon dioxide ( EtCO2 ) monitoring is considered to reflect real - time estimation of partial pressure of carbon dioxide in arterial blood ( PaCO2 ) noninvasively . - pubmed
Consequently , the antagonistic A2A - D2 receptor interactions in a putative striatal receptor heteromer on striato - pallidal GABA neuron led to the introduction of A2A receptor antagonists as possible anti- Parkinsonian drugs . - pubmed
Special emphasis is given to in vivo microdialysis findings demonstrating the functional role putative A2A / D2 heteromers on striato - pallidal GABA neurons play in the modulation of this pathway , in which A2A receptors inhibit D2 receptor signaling . - pubmed
However , this does not necessarily lead to type I IFN production or autoimmunity unless there is concomitant exposure to endogenous TLR-7 ligands , which are released from dead cells following pristane treatment . - pubmed
Circadian changes in phosphate were not explained by urine phosphate excretion , parathyroid hormone , or fibroblast growth factor-23 . - pubmed
The main such transporters include Na (+)/ H (+ ) exchangers , various HCO3 (- ) transporters , H (+ ) pumps , and lactate - H (+ ) cotransporters . - pubmed
A water - soluble cationic conjugated polyelectrolyte ( CPE ) , poly ( 1,4 - bis ( 6 -( 1 - methylimidazolium )- hexyloxy )- benzene bromide ) ( PMI ) displays extraordinary stability over the full pH range of 1 - 14 as well as in seawater , brine , urine , and other solutions and carries out efficient detection , discrimination , and removal of moderately dissimilar anionic surfactants ( viz . - pubmed
Using PMI , a newly developed CPE , a rapid and practical method for the efficient detection , discrimination , and removal of SDS and SDBS at ppb levels from water and urine , under harsh conditions , and in natural chemical environments is demonstrated . - pubmed
In the MTA group , none of the mixing techniques exhibited a significant effect on CS ( P>0.05 ) ; however , in CEM group the CS at 21 - h and 21 - day intervals was higher with the hand technique ( P<0.05 ) . - pubmed
Telescopic vs. balloon showed : post - operative pain - day one 2.5 vs. 2.5 ; p = 0.90 , day seven 0 vs. 0 ; p = 0.02 ( 0 vs. 1 ; p & lt ; 0.01 for bilateral hernias ) ; operation times - unilateral 48.0 vs. 48.0 min ; p = 0.88 and bilateral 70.0 vs. 65.0 min ; p = 0.66 , length of hospital stay 1.0 vs. 1.0 day , analgesic intake ( dextropropoxyphene ) 6.0 vs. 6.0 tablets ; p = 0.95 , return to work / normal physical activities 7.0 vs. 7.0 days ; p = 0.46 and cosmetic scar scores 24.0 vs. 24.0 , respectively . - pubmed
This response could be mimicked by the addition of Asymmetric Dimethylarginine ( ADMA ) , an endogenous inhibitor of NO synthesis and the substrate for both isoforms of DDAH . - pubmed
WIDER IMPLICATIONS OF THE FINDINGS : This study provides new insight into the role of the DDAH / ADMA pathway in the regulation of trophoblast function . - pubmed
Both dysregulation of DDAH and the accumulation of ADMA have been associated with the development of pre - eclampsia . - pubmed
This is the first study to implicate the DDAH / ADMA pathway as a mechanism that might underlie the poor trophoblast invasion seen in this common pregnancy disorder . - pubmed
STIM1 elevation in the heart results in aberrant Ca2 + handling and cardiomyopathy . - pubmed
Stromal interaction molecule 1 ( STIM1 ) is a Ca ( 2 + ) sensor that partners with Orai1 to elicit Ca ( 2 + ) entry in response to endoplasmic reticulum ( ER ) Ca ( 2 + ) store depletion . - pubmed
While store - operated Ca ( 2 + ) entry ( SOCE ) is important for maintaining ER Ca ( 2 + ) homeostasis in non - excitable cells , it is unclear what role it plays in the heart , although STIM1 is expressed in the heart and upregulated during disease . - pubmed
As expected , STIM1 transgenic myocytes showed enhanced Ca ( 2 + ) entry following store depletion and partial co - localization with the type 2 ryanodine receptor ( RyR2 ) within the sarcoplasmic reticulum ( SR ) , as well as enrichment around the sarcolemma . - pubmed
Associated with this enhanced Ca ( 2 + ) profile was an increase in cardiac nuclear factor of activated T - cells ( NFAT ) and Ca ( 2 +)/ calmodulin - dependent kinase II ( CaMKII ) activity . - pubmed
We conclude that STIM1 has an unexpected function in the heart where it alters communication between the sarcolemma and SR resulting in greater Ca ( 2 + ) flux and a leaky SR compartment . - pubmed
Second - Trimester Maternal Serum Paraxanthine , CYP1A2 Activity , and the Risk of Severe Preeclampsia . - pubmed
OBJECTIVE : To measure the association between second - trimester maternal caffeine intake and caffeine metabolism through the CYP1A2 system and the risk of subsequent severe preeclampsia . - pubmed
The outcome of interest was severe preeclampsia , and exposures were serum paraxanthine ( 1,7 - dimethylxanthine ) , measured through high - performance liquid chromatography , and CYP1A2 activity , assessed by paraxanthine / caffeine ratios . - pubmed
In erythrocytes made echinocytic with exogenous phospholipid , addition of hemoglobin oxidized with hydrogen peroxide ( H2O2 ) or Vitamin C ( conditions that result in the formation of significant quantities of choleglobin ) , but not ferricyanide ( which produces predominantly methemoglobin ) , induced dose - dependent shape reversion to less echinocytic forms , consistent with extraction of phospholipids from the exofacial side of the membrane . - pubmed
Erythrocytes preloaded with radiolabeled phosphatidylcholine or NBD - labeled phosphatidylcholine , phosphatidylglycerol or phosphatidic acid , exhibited greatest extraction of radiolabel or fluorescence signal with exogenous hemoglobin oxidized via H2O2 or Vitamin C , but not ferricyanide . - pubmed
However , with NBD - phosphatidylserine ( a preferential inner monolayer intercalator ) , significantly less extraction of labeled lipid occurred with oxidized hemoglobin prepared under all three oxidizing conditions . - pubmed
In dimyristoylphosphatidylcholine liposomes containing radiolabeled phosphatidylcholine , phosphatidylserine or phosphatidylethanolamine , subsequent addition of hemoglobin oxidized with H2O2 or Vitamin C extracted radiolabeled lipid with significantly greater efficiency than ferricyanide - treated hemoglobin , with enhanced extraction detectable at levels approaching physiological plasma oxidant concentrations . - pubmed
Hemoglobin dimerization occurred at very low levels with H2O2 treatment , and even lower levels with Vitamin C treatment , and thus did not correlate to the high efficiency and consistent levels of lipid extraction observed with these treatments . - pubmed
These findings indicate that choleglobin extracts lipids from cell membranes regardless of headgroup or acyl chain length , through a process of direct hydrophobic interaction with the membrane surface . - pubmed
The immunological effects of rapamycin and BM - MSC treatments were associated with the inhibition of the Ag - specific lymphocyte proliferation , CD8 + cytolytic activity , and the Th1 - type cytokine ( gamma - interferon ( IFN - gamma ) ) and the increase of Th-2 cytokine ( interleukin-4 ( IL-4 ) and IL-10 ) production . - pubmed
This suggests the potential of rapamycin and BM - MSC combined therapy to play neuroprotective roles in the treatment of neuroinflammatory disorders . - pubmed
The blood glucose ( BG ) level over a 30 - minute interval in Week 2 was relatively the same as that in Week 1 , suggesting that EA treatment does not increase the likelihood of developing hyperglycemia . - pubmed
BACKGROUND : The aim of this study was to investigate the relationship between serum triglycerides ( TG ) levels and atherosclerosis and to explore its predicated value for atherosclerosis in elderly Chinese population . - pubmed
The aim of this study was to investigate whether CS / DS rich in GalNAc(4,6SO4 ) residues participate in the progression or resolution of liver fibrosis . - pubmed
ATM / CHK / p53 Pathway Dependent Chemopreventive and Therapeutic Activity on Lung Cancer by Pterostilbene . - pubmed
To investigate the underlying mechanism of this superior action of pterostilbene on cancer cells , we utilized a reverse - phase protein array followed by bioinformatic analysis and found that the ATM / CHK pathway is modified by pterostilbene in a lung cancer cell line . - pubmed
Given that ATM / CHK signaling requires p53 for its biological effects , we hypothesized that p53 is required for the anticancer effect of pterostilbene . - pubmed
Pterostilbene also activated ATM and CHK1 / 2 , which are upstream of p53 , in both cell lines , though pterostilbene - induced senescence was dependent on the presence of p53 . - pubmed
Finally , pterostilbene more effectively inhibited p53 - dependent cell proliferation compared to the other three stilbenoids . - pubmed
These results strongly support the potential chemopreventive effect of pterostilbene on p53 - positive cells during early carcinogenesis . - pubmed
Hydrostatic pressure applied using diamond anvil cells ( DAC ) has been widely explored to modulate physical properties of materials by tuning their lattice degree of freedom . - pubmed
Multiple - terminal FETs were fabricated in the DAC using few - layer MoS2 and BN as the channel semiconductor and dielectric layer , respectively . - pubmed
We intended to identify a set of structurally diverse hits , which occupy all subsites ( S1'-S3 ' , S2 , and S3 ) centering the zinc containing binding site of MMP-13 , by the virtual screening of a chemical library comprising more than ten million commercially available compounds . - pubmed
Exogenous diacylglycerol leads to membrane translocation of PKC , indicative of an activation . - pubmed
The intima and media layers were obtained by sequentially electrospinning silk fibroin ( SF ) and poly ( L - lactide - co - epsilon - caprolactone ) , with ratios selected to match the mechanical properties of the native tissue . - pubmed
The discovery that the most abundant cholesterol oxidized metabolite in the plasma , 27 hydroxycholesterol ( 27 - OHC ) , is a selective estrogen receptor modulator ( SERM ) and an agonist of Liver X receptors ( LXR ) partially fills the void in our understanding and knowledge of the mechanisms that may link hypercholesterolemia to development and progression of breast cancer and PCa . - pubmed
The wide spectrum and repertoire of SERM and LXR - dependent effects of 27 - OHC in the context of all facets and aspects of breast cancer and prostate cancer biology are reviewed in this manuscript in a very comprehensive manner . - pubmed
This review highlights recent findings pertaining to the role of 27 - OHC in breast cancer and PCa and delineates the signaling mechanisms involved in the governing of different facets of tumor biology , that include tumor cell proliferation , epithelial - mesenchymal transition ( EMT ) , as well as tumor cell invasion , migration , and metastasis . - pubmed
The plasma H2S levels , apoptosis of esophageal - gastric junction vascular smooth muscle cells , and the expression of nuclear transcription factor - kappaB ( NF - kappaB ) , p - AKT , IkappaBa and Bcl-2 were detected . - pubmed
The results showed that the plasma H2S levels and the CSE expression levels had statistically significant difference among different groups ( P<0.05 ) . - pubmed
As compared with PH group , plasma H2S levels were declined obviously ( 11.9 + -4.2 vs. 20.6 + -4.5 , P<0.05 ) , and CSE expression levels in the junction vascular tissue were notably reduced ( 1.7 + -0.6 vs. 2.8 + -0.8 , P<0.05 ) , apoptosis rate of vascular smooth muscle cells per unit area was significantly decreased ( 0.10 + -0.15 vs. 0.24 + -0.07 , P<0.05 ) , and the expression levels of p - AKT and NF - kappaB were significantly decreased ( 2.31 + -0.33 vs. 3.04 + -0.38 , P<0.05 ; 0.33 + -0.17 vs. 0.51 + -0.23 , P<0.05 ) , however , IkappaBa and Bcl-2 expression increased obviously ( 5.57 + -0.17 vs. 3.67 + -0.13 , P<0.05 ; 0.79 + -0.29 vs. 0.44 + -0.36 , P<0.05 ) in PH+PPG group . - pubmed
As compared with PH group , H2S levels were notably increased ( 32.7 + -7.3 vs. 20.6 + -4.5 , P<0.05 ) , the CSE levels in the junction vascular tissue were significantly increased ( 6.3 + -0.7 vs. 2.8 + -0.8 , P<0.05 ) , apoptosis rate of vascular smooth muscle cells per unit area was significantly increased ( 0.35 + -0.14 vs. 0.24 + -0.07 , P<0.05 ) , and the expression levels of p - AKT and NF - kappaB were significantly increased ( 4.29 + -0.49 vs. 3.04 + -0.38 , P<0.05 ; 0.77 + -0.27 vs. 0.51 + -0.23 , P<0.05 ) , yet IkappaBa and Bcl-2 expression decreased significantly ( 3.23 + -0.24 vs. 3.67 + -0.13 , P<0.05 ; 0.31 + -0.23 vs. 0.48 + -0.34 , P<0.05 ) in PH+S group . - pubmed
H2S can activate NF - kappaB by the p - AKT pathway , leading to the down - regulation of Bcl-2 , eventually stimulating apoptosis of vascular smooth muscle cells , easing PH . - pubmed
H2S / CSE system may play an important role in remission of PH via the AKT - NF - kappaB pathway . - pubmed
MR is expressed in all cell types in the heart , including the endothelial cells ( EC ) , in which aldosterone induces the expression of intercellular adhesion molecule 1 ( ICAM-1 ) . - pubmed
This pregnancy - related analgesia is reversible by intrathecal administration of naloxone , suggesting an opioid - mediated mechanism ; pharmacological and genetic data suggest the importance of delta - opioid receptors . - pubmed
We show that the phenomenon is dependent on pregnancy hormones ( estrogen and progesterone ) , delta - opioid receptors , and T cells of the adaptive immune system . - pubmed
The extracellular , active 180 amino acid Nogo - A region , named Nogo - A - Delta20 , binds to heparin and brain - derived heparan sulfate glycosaminoglycans ( GAGs ) but not to the closely related chondroitin sulfate GAGs . - pubmed
Ultrasound - assisted facile one - pot sequential synthesis of novel sulfonamide - isoxazoles using cerium ( IV ) ammonium nitrate ( CAN ) as an efficient oxidant in aqueous medium . - pubmed
A series of novel 3,5 - disubstituted isoxazoles have been synthesized , using a new , green , and versatile " one - pot three - steps " methodology . - pubmed
The syntheses are highly regioselective , as illustrated by the structures of the final compounds , which have been fully assessed by spectral analyses ( 1H and 13C NMR , MS ) . - pubmed
GP : T and DN :P SI ratios were compared between groups by using the analysis of variance and were analyzed relative to group , total cumulative number of doses of GBCA , age , and sex by using multivariable linear models . - pubmed
Concerted action of dipeptidyl peptidase IV and glutaminyl cyclase results in formation of pyroglutamate - modified amyloid peptides in vitro . - pubmed
The subsequent transformation of resulted Abeta ( 3 - 40 / 42 ) to pE - Abeta ( 3 - 40 / 42 ) in QC catalyzed glutamate cyclization was manifested . - pubmed
The best mucilage production from callus ( 36.76 % g g-1 dry weight ) was obtained in the MS medium supplemented with 1 mg L-1 of 2 , 4 - D and 2 mg L-1 of BAP under the light condition . - pubmed
The supplementation of emulsifier in the low - energy diet linearly decreased cholesterol ( P = 0.099 ) and LDL / C ( P = 0.074 ) . - pubmed
Based on the BAS score , BD who were moderately BAS - affected ( n = 5 ) , had significantly lower arterial saturation of hemoglobin with oxygen values on arterial blood gas when compared with MDD ( n = 18 ) . - pubmed
Although TRPV1 expressing neuron percentage was increased in Ins2+/Akita DRGs at 9 months of diabetes compared to control , capsaicin - induced Ca2 + influx was smaller in isolated Ins2+/Akita DRG neurons , indicating impaired TRPV1 function . - pubmed
Patch - clamp recordings revealed that capsaicin - activated currents decayed faster in isolated Ins2+/Akita DRG neurons as compared to those in control neurons . - pubmed
We propose that in poorly controlled diabetes , the accelerated rate of capsaicin - sensitive TRPV1 current decay in DRG neurons decreases overall TRPV1 activity and contributes to peripheral neuropathy . - pubmed
It has been shown previously in formaldehyde crosslinking experiments that EloR co - precipitates with KhpA , indicating that they form a complex in vivo . - pubmed
Site directed mutagenesis identified isoleucine 61 ( I61 ) as crucial for KhpA homodimerization . - pubmed
When substituting I61 with phenylalanine , KhpA lost the ability to homodimerize , while it still interacted clearly with EloR. - pubmed
Novel PSMA - Coated On - Off - On Fluorescent Chemosensor Based on Organic Dots with AIEgens for Detection of Copper ( II ) , Iron ( III ) and Cysteine . - pubmed
The active esters in the non - conjugated vinyl monomers could be used in a post - polymerization functionalization step to create functionalized polymers with defined monomer sequences in a modular way . - pubmed
The porosity of the cement paste , the aggregate and the interfacial transitional zones ( ITZ ) of a PC with recycled coarse aggregates ( RCA ) and silica fume ( SF ) is measured by means of image analysis - scanning electron microscope ( IA)-(SEM ) and by mapping the chemical elements with an SEM - EDS ( energy dispersive spectrometer ) detector microanalysis linked to the SEM and , as a contrast , the mercury intrusion porosimetry technique ( MIP ) . - pubmed
Cadmium is an environmental contaminant that can activate estrogen receptor alpha ( ERalpha ) and contribute to the development and progression of breast cancer . - pubmed
MTT and TTP were significantly higher and CBF was significantly lower when the number of LSAs was between 5 and 10 on each side of the basal ganglia ( P & lt ; 0.001 , & lt;0.001 , and & lt;0.001 , respectively ) . - pubmed
The three most prevalent serovars in the MAT were selected for the development of a pooled antigen for use in MSAT ; subsequently , the 193 serum were assessed with the macroscopic slide agglutination test ( MSAT ) containing the developed antigen . - pubmed
Celastrol - induced degradation of FANCD2 sensitizes pediatric high - grade gliomas to the DNA - crosslinking agent carboplatin . - pubmed
FINDINGS : FANCD2 is overexpressed in HGGs and depletion of FANCD2 by celastrol synergises with carboplatin to induce cytotoxicity . - pubmed
In addition , our results suggest that celastrol treatment stalls ongoing replication forks , causing sensitivity to DNA - crosslinking in FANCD2 - dependent glioma cells . - pubmed
lncRNA ZEB1 - AS1 inhibits high glucose - induced EMT and fibrogenesis by regulating the miR-216a-5p / BMP7 axis in diabetic nephropathy . - pubmed
lncRNA ZEB1 - AS1 inhibited high glucose - induced EMT and fibrogenesis via regulating miR-216a-5p / BMP7 axis in diabetic nephropathy , providing a potential target for DN therapy . - pubmed
Zinc ferrite and copper oxide nanostructure ( ZnFe2O4 - CuO ) and gold nanoparticles ( AuNPs ) have been used to prepare a nanocomposite in modifying a glassy carbon electrode ( GCE ) . - pubmed
The unique ZnFe2O4 - CuO / Au nanocomposite was applied as a sensor for the determination of traces of MET by some electroanalytical techniques . - pubmed
Under the optimum conditions and at a working potential of 0.85 V ( vs. Ag / AgCl/3.0 M KCl ) , the sensor response is linear in the MET range of 1.0 nmol L-1 to 1.0 mumol L-1 MET . - pubmed
Hydrogenase genes were also widespread in samples from three other climatically distinct deserts , the Namib , Gobi , and Mojave , and atmospheric H2 oxidation was also greatly stimulated by hydration at these sites . - pubmed
AIM : This study aimed to assess the effect of addition of nano - titanium oxide ( nano - TiO2 ) , nano - silicon dioxide ( nano - SiO2 ) , and a combination of both , on antimicrobial activity of an orthodontic composite . - pubmed
Warming crude ratuterine cytosol after the addition of [ 3H ] estradiol accelerates the association of the 4 - S estrogen - binding protein with a second macromolecule , resulting in the formation of the 5 - S estrogen - binding protein . - pubmed
Following each of these methods , the fraction containing the 4 - S estrogen - binding protein was incubated at 28 degrees C ; each of the these 4 - S estrogen - binding protein - containing fractions retained its capacity to completely transform to the 5 - S estrogen - binding protein . - pubmed
In samples subjected to partial purification procedures , it was necessary that the buffer contain 40 mM Tris , 60 mM Tris , 60 mM KC1 , 1 - 10 MM dithiothreitol , and 1 M urea at pH 7.4 , in order to accomplish the 4 - S to 5 - S estrogen - binding protein transformation at 25 degrees C. Formation of the 5 - S estrogen - binding protein requires association of the 4 - Estrogen - binding protein with a molecule identical to or very similar to itself . - pubmed
Another population , T2 , is long - lived after ATx , but highly sensitive to elimination by small doses of ATS . - pubmed
From precursors within the T2 population , effectors of specific helper activity , after priming with antigen , appeared within 1 to 2 days and reached a maximum on day 4 . - pubmed
[ Detection of anti - idiotypic antibody specific for anti - HBs in HBsAg positive serum ] . - pubmed
An ELISA for detecting anti - idiotypic antibody specific for anti - HBs(anti - anti - HBs , Ab 2 ) was devised and used to detect twenty - three asymptomatic HBsAg positive sera . - pubmed
Of these HBsAg positive samples , five were positive for anti - anti - HBs . - pubmed
The authors suggested that samples with HBsAg positive , especially those from asymptomatic carriers should be detected for anti - anti - HBs in order to rule out the interference of anti - anti - HBs . - pubmed
The aberrant PML proteins lacked the C terminus , which had been replaced by from two to ten amino acid residues from the RAR alpha sequence . - pubmed
Findings supported the idea that endogenous lectins could interact with sugar residues and perform several roles including packaging , immobilization and transport of glucidic material . - pubmed
Plasma parathormone ( PTH ) and urinary cyclic AMP ( ur . - pubmed
Response was assessed by barium swallow , CT scan , and measurement of metastases where present . - pubmed
In this study we have examined the effects of the diterpene forskolin on various parameters of B cell activation . - pubmed
Cells were activated either by antibodies to surface immunoglobulins ( anti - mu ) , by the monoclonal antibody 1F5 reactive with the CD20 antigen or by 12 - O - tetradecanoylphorbol 13 - acetate . - pubmed
While anti - mu stimulation induces increased phosphatidylinositol ( PI ) turnover and [ Ca2+]i fluxes , the latter two reagents confer an activation of B cells independent of the PI / Ca2 + pathway . - pubmed
We found a clear inhibitory effect of forskolin on the anti - mu - induced PI turnover and [ Ca2+]i fluxes as well as on later parameters of cell activation . - pubmed
There was also a clear inhibition of G1 entry and DNA synthesis when PI / Ca2 + -independent activation was employed , indicating that cAMP interferes with B lymphocyte stimulation in several ways . - pubmed
Importantly , forskolin maintained its inhibitory effect when added late after anti - mu stimulation , implying an effect also at multiple stages of activation . - pubmed
In the present study , we examine regulation of the isoenzymes by thyroid hormone , which is well known to increase activity of Na +- K +- ATPase in different tissue preparations . - pubmed
The number of alpha(+)- and alpha - subunits in heart homogenates estimated by [ 3H]ouabain binding was 3.5 + /- 0.1 and 2.5 + /- 0.1 pmol / mg protein , respectively [ alpha(+)/alpha = 1.40 ] . - pubmed
Capillary blood glucose concentrations before breakfast ( 5.2 + /- 0.4 mmol / l vs 9.1 + /- 0.8 mmol / l ) , after lunch ( 6.5 mmol / l + /- 0.8 vs 7.9 + /- 1.0 mmol / l ) and before the evening meal ( 5.0 + /- 0.7 mmol / l vs 7.7 + /- 0.7 mmol / l ) were lower on CSII , as were 24 - hour urine glucose excretion and total insulin dose ( 39.3 + /- 2.2 vs 49.8 + /- 4.0 U / day ) . - pubmed
There was a significant positive correlation between fasting blood glucose values and glycosylated haemoglobin on injection but not pump treatment . - pubmed
Its action on the cAMP - dependent protein kinase was found to be competitive with regard to protein substrate , but noncompetitive with regard to ATP and cAMP . - pubmed
For that reason , positive post - narcotic physostigmine effects could be due to its anti - opioid potency as well . - pubmed
The organism has been categorized into 10 biotypes based on the variable fermentation of dextrin , maltose , mannitol , and xylose and into 3 serotypes on the basis of heat stable , cell surface antigens . - pubmed
[ On the effect of trypsin , chymotrypsinogen , chymotrypsin , and amylase on iron resorption ] . - pubmed
[ Adrergic beta - receptor blocking with propranolol in some disorders of cardiac rhythm ] . - pubmed
While the demonstration of the in vivo inhibitory activity of n - butyl malonate and hydroxycitrate is entirely by physiological methods , that of the transaminase inhibitors aminooxyacetate and cycloserine depends in part on the isolation of mutants capable of growth on glycerol in minimal medium . - pubmed
On this medium these mutants depend on the malate - aspartate shuttle for growth , and as expected the transaminase inhibitors prevent their growth . - pubmed
Asparagine - linked glycopeptides were found to be mainly composed of the complex bi - antennary type as shown by affinity chromatography on concanavalin - A Sepharose ; high - molecular - weight glycopeptides were represented by nonfucosylated lactosaminoglycans since they were metabolically labeled with [ 14C ] glucosamine but not with [ 3H ] fucose , did not bind to DEAE - cellulose , and were susceptible to endo - beta - galactosidase . - pubmed
Labeling with galactose oxidase / Na B3H4 technique demonstrated that lactosaminoglycans were present on the surface of differentiating germ cells and of testicular and epididymal spermatozoa . - pubmed
On the other hand , epididymal spermatozoa lactosaminoglycans bound to the lectin and therefore contained fucose . - pubmed
A soluble fucosyltransferase , capable of transferring fucose to germ cell lactosaminoglycans , was found to be present in the epididymis but not in the testis . - pubmed
We suggest that these molecules are fucosylated in vivo by a fucosyltransferase secreted by the epididymal epithelium . - pubmed
Erythropoiesis was quantitated by reticulocyte response , ferrokinetics ( plasma iron turnover and marrow transit time ) , and by hemoglobin C synthesis . - pubmed
The sarcoplasmic reticulum Ca ( 2 +)- ATPase loses hydrolytic activity and the ability to be phosphorylated by Pi following incubation with EDC [ 1 - ethyl-3 -( 3 - dimethylaminopropyl ) carbodiimide ] . - pubmed
4 nmol of tempamine per mg SR protein can be coupled to either a glu or an asp side chain through the EDC reaction . - pubmed
In multivariate analysis , VLDL and global coronary atherosclerosis score predicted reinfarction ; plasma plasminogen activator inhibitor-1 ( PAI-1 ) activity and global coronary stenosis score predicted cardiac death ; and VLDL triglyceride levels , global coronary atherosclerosis score and age predicted any major coronary event . - pubmed
The oxygen - binding binuclear center of cytochrome bo is located in subunit I and consists of a heme ( heme o ; heme a3 in the aa3 - type oxidases ) and a copper ( Cu ( B ) ) . - pubmed
Three other amino acid residues which are potential ligands for heme o are shown tob e nonessential for enzyme activity . - pubmed
These glycans can be released by treatment with endoglycosidase H , and the majority has the same size as Man5GlcNAc1 glycans . - pubmed
thaliana with a transgenic strain that expresses the glycoprotein phytohemagglutinin , we obtained a unique strain that synthesizes phytohemagglutinin with two high - mannose glycans , instead of one high - mannose and one complex glycan . - pubmed
Interaction of myosin with charged membranes is affected by ATP . - pubmed
Total lysophosphatidylcholine concentration ( sum of lysophosphatidylcholine remaining in tissue and lysophosphatidylcholine released into the buffer ) increased from 0.73 + /- 0.08 nmol / mg protein at baseline to 1.83 + /- 0.45 nmol / mg protein after 5 minutes of ischemia ( p & lt ; 0.001 ) , and was associated with evidence of cell injury ( 26 % depletion of tissue lactate dehydrogenase ) . - pubmed
In contrast , there was no lysophosphatidylcholine accumulation or release and no lactate dehydrogenase depletion in oxygenated and perfused controls . - pubmed
Attenuation of lysophosphatidylcholine accumulation by incubation with lysophospholipase did not prevent cell injury . - pubmed
This database indicated that groundwater concentrations of TCE typically exceeded EPA 's MCL of 5 ppb for TCE . - pubmed
The presence of longitudinal sinus thrombosis was suggested by unenhanced CT scan , confirmed by colour doppler flow imaging and magnetic resonance angiography , with absence of blood flow in the longitudinal sinus . - pubmed
Both prenatal halothane and TFAA exposure produced changes in liver biochemistry of newborns , as indicated by significant increases in the serum activities of glutamate dehydrogenase and aspartate aminotransferase . - pubmed
In addition , TFAA caused a functional deficit of the proximal tubule in newborns , as evidenced by the significant increase in the urinary excretion of beta 2 - microglobulin . - pubmed
As a group ( n = 56 ) , RAR activity increased from a baseline of 1.2 + /- 0.2 to a peak of 4.3 + /- 0.3 impulses / breath at 8 - 10 min after the onset of PAE induction . - pubmed
Studies were repeated in 33 RARs initially stimulated by PAE . - pubmed
In another 24 receptors , the responses of RARs to PAE were unaltered by pretreatment with saline ( n = 8) or dimethylthiourea ( a hydroxyl radical scavenger ; n = 8) , but was significantly attenuated by pretreatment with ibuprofen ( a cyclooxygenase inhibitor : n = 8) . - pubmed
These results suggest that : ( 1 ) the increased RAR activity following PAE is associated primarily with an increase in bronchomotor tone and secondarily with the mechanical action of the beating heart impacting on lung tissues ; and ( 2 ) cyclooxygenase products may be involved in this sensory stimulation . - pubmed
Adenosine receptor blockade with 8 - phenyltheophylline ( 8 - PT ) was used to shift the adenosine dose - response curve 12 - fold . - pubmed
During intracoronary norepinephrine infusion , coronary blood flow and myocardial oxygen consumption increased similarly before and after 8 - PT , demonstrating a lack of an effect from the adenosine receptor blockade . - pubmed
After 8 - PT , a similar increase in estimated interstitial adenosine was found , demonstrating that there was no augmentation in adenosine concentration to overcome the adenosine receptor blockade . - pubmed
Thus adenosine could not be responsible for the increase in coronary blood flow after adenosine receptor blockade and therefore is not required for norepinephrine - induced hyperemia . - pubmed
This was demonstrated with the use of blocking antibodies directed to alpha4beta1 and peptide adhesion competitors containing the alpha4beta1 binding tripeptide , Leu - Asp - Val ( LDV ) . - pubmed
Consistent with this data , the adhesion is completely divalent cation - dependent and is stimulated by known activators of leukocyte integrin function , namely phorbol ester and the beta1 integrin activating antibody , 8A2 . - pubmed
The pH dependence of the model results from the binding of hydrogen ions to TM macromolecules -- to both neutral sites with basic pK and negatively charged sites with acidic pK. - pubmed
The exposure of the tyrosyl component is less in apo - TL than in holo - TL . - pubmed
The sole tryptophan residue , Trp17 , is buried in both holo - and apo - TL . - pubmed
The steady state exposure of Trp17 is the same in holo- and apo - TL , but the dynamic exposure is two - fold greater in apo - TL . - pubmed
Maneuvers to unfold the protein with urea or incubation in an acidic environment resulted in increased exposure of aromatic amino acids . - pubmed
Meta - analysis of streptokinase and heparin in deep vein thrombosis . - pubmed
The efficacy and safety of streptokinase and heparin in deep vein thrombosis ( DVT ) were compared in a meta - analysis . - pubmed
Randomized trials in which streptokinase ( followed by heparin ) and heparin alone were compared in treating phlebographically documented acute DVT were identified from MEDLINE and other sources for a meta - analysis . - pubmed
A fixed - effect model was used to combine the study results , enabling differences between streptokinase recipients and recipients of heparin alone to be expressed as summary ORs with 95 % CIs . - pubmed
Significantly more streptokinase recipients achieved thrombolysis than recipients of heparin alone ( summary OR , 6.24 ; 95 % CI , 3.62 to 10.78 ) . - pubmed
The frequency of major bleeding was significantly higher among streptokinase recipients than recipients of heparin alone ( summary OR , 3.78 ; 95 % CI , 1.26 to 11.32 ) . - pubmed
A meta - analysis showed that , compared with heparin alone , streptokinase therapy for DVT was associated with significantly more frequent thrombolysis and major bleeding ; after exclusion of one outlying study , analysis showed that streptokinase therapy was associated less frequently than heparin alone with postthrombotic changes ( assessed by phlebographic evaluation ) and postphlebitic syndrome . - pubmed
P450BM-3 : absolute configuration of the primary metabolites of palmitic acid . - pubmed
P450BM-3 catalyzes the oxidation of short- and long - chain , saturated and unsaturated fatty acids . - pubmed
While the 15 - and 14 - hydroxy compounds are produced in a highly stereoselective manner ( 98 % R , 2 % S ) , the 13 - hydroxy is a mixture of 72 % R and 28 % S. We have also examined the binding of these three hydroxy acids to P450BM-3 and shown that only two of them ( 14 - OH and 13 - OH palmitic acid ) can bind to and be further metabolized by P450BM-3 . - pubmed
The results indicate that in contrast to the flexibility of palmitoleic acid bound to the oxidized enzyme , palmitic acid is rigidly bound in the active site during catalytic turnover . - pubmed
All major classes of antihypertensive agents ( calcium channel blockers , diuretics , beta - blockers , angiotensin - converting enzyme inhibitors ) can cause LVH regression but not all to the same degree . - pubmed
Angiotensin - converting enzyme inhibitors may provide the most pronounced reduction in left ventricular mass per millimeter of mercury of blood pressure reduction . - pubmed
Because ARBs act specifically on the AT ( 1 ) receptor , angiotensin II can exert its favorable effects on cell growth inhibition through the AT ( 2 ) receptor . - pubmed
coli lacking endogenous cysteine synthase genes could functionally rescue the growth without addition of cysteine . - pubmed
The activity of recombinant serine acetyltransferase produced from the cDNA was inhibited by L - cysteine , which is the end - product of the pathway ; however , the sensitivity to cysteine ( 48.7 microM of the concentration for 50 % inhibition , IC(50 ) ) was fairly low compared with that of previously reported serine acetyltransferases ( approximately 5 microM IC(50 ) ) from various plants . - pubmed
tuberosum is likely due to the lower sensitivity of feedback inhibition of serine acetyltransferase to cysteine . - pubmed
The analysis of the predicted amino acid sequence of the cDNA clone revealed that the encoded protein contains two RNA binding domains , including the conserved consensus ribonucleoprotein sequences CS - RNP1 and CS - RNP2 , on the C - terminus half and the presence of a putative transit peptide sequence in the N - terminus region . - pubmed
17betaE(2 ) and bio-17betaE(2 ) , but not other hormones , were correlated with BMD after adjustment for age and body weight . - pubmed
Based on previous studies , unfiltered sulfur colloid provided a higher success rate of SN identification than the other tracer types . - pubmed
Further evaluation with 99mTc - sulfur colloid demonstrated that increased volume increased the success rate of SN identification . - pubmed
Electrophoretic mobility shift experiments with primary Th ( T helper ) cells showed the formation of a specific , T - cell receptor - inducible complex at this site that is sensitive to cyclosporin A and supershifted with anti - NFATc2 in both Th1 and Th2 cells . - pubmed
This toxin , named OsK2 , has been characterized as a 28 - residue peptide , containing six conserved cysteine residues and was shown to be a potent and selective blocker of Kv1.2 channels ( K(d ) = 97 nM ) . - pubmed
OsK2 is the second member of the 13th subfamily of short - chain K (+ ) channel - blocking peptides known thus far and is therefore called alpha - KTx 13.2 . - pubmed
( D'Arcy ) secrete large amounts of 2,3,4 - tri - O - acylglucoses possessing straight- and branched - chain fatty acids of short to medium chain length ( C4 - C12 ) . - pubmed
These fused - ring polyketides are formed by the action of polyketide synthases ( PKSs ) , which catalyse the assembly , folding and cross - linking of poly - beta - ketoacyl intermediates . - pubmed
Adenosine or the selective A1 receptor agonist R - PIA causes a concentration - dependent contraction . - pubmed
After pretreatment with A1 receptor antagonist , DPCPX , adenosine - mediated contraction was abolished . - pubmed
Adenosine - induced contraction was significantly increased when A1 receptors were preserved by pretreatment with DPCPX followed by inactivation of all unprotected receptors with N - ethylmaleimide . - pubmed
Adenosine- or R - PIA - induced contraction was significantly augmented in the preserved cells and the increase was abolished in the presence of the A1 receptor antagonist DPCPX . - pubmed
PTX abolished contraction induced by adenosine or R - PIA , implying that contraction activated by A1 receptor was coupled to a pertussis toxin ( PTX)-sensitive G ( i ) protein . - pubmed
Adenosine- or R - PIA - induced contraction of esophageal smooth muscle cells was not affected by the phospholipase D ( PLD ) inhibitor rho - chloromercuribenzoic acid ( rhoCMB ) , phospholipase A ( 2 ) ( PLA ( 2 ) ) inhibitor DEDA or PKC antagonist chelerythrine , but was significantly abolished by phospholipase C ( PLC ) inhibitor , neomycin . - pubmed
PLC - beta3 antibody inhibited R - PIA - induced contraction . - pubmed
R - PIA - induced contraction of esophageal muscle cells was inhibited by IP ( 3 ) receptor antagonist heparin , which suggests that the contraction of esophageal smooth muscle cells is dependent on phosphatidylinositol - specific phospholipase ( PI - PLC ) and IP ( 3 ) . - pubmed
A1 receptor is coupled to PTX - sensitive G protein G ( i2 ) , which results in the activation of PI - PLC - beta3 . - pubmed
PI hydrolysis by PI - PLC forms IP(3 ) , which binds to IP ( 3 ) receptor on endoplasmic reticulum , resulting in the release of intracellular Ca ( 2 + ) . - pubmed
A reversed - phase liquid chromatography ( LC ) method has been developed , optimised and validated for the separation and quantitation of capsaicin ( CP ) and dihydrocapsaicin ( DHCP ) in a topical cream formulation . - pubmed
The conditions allow the separation and quantitation of CP and DHCP without interference from the other substances contained in the cream . - pubmed
A technetium 99m - labeled tetrofosmin stress - rest tomographic scan and iodine 123 - labeled metaiodobenzylguanidine planar scans were performed before and after transmyocardial laser revascularization ( 3 and 12 months later ) to evaluate myocardial perfusion and innervation . - pubmed
Liver and muscle tissues were isolated from exposed , recovery and control fish at the end of 1 , 3 and 6 days in each condition , and used for the estimation of protein , amino acids , ammonia , glycogen , aminotransaminases , glycogen phosphorylase ( a and ab ) and glutamate dehydrogenase . - pubmed
The glycogen phosphorylase ab activity in liver was suppressed on exposure to carbofuran . - pubmed
Significantly , specific inhibition of GSK-3 activity by LiCl prevents hyperphosphorylation of tau , and spatial memory impairment resulting from PI3 K and PKC inhibition . - pubmed
Thyroid hormone induces erythropoietin gene expression through augmented accumulation of hypoxia - inducible factor-1 . - pubmed
Hypoxia , the reduction of oxygen supply , results in adaptationally appropriate alterations in gene expression through the activation of hypoxia - inducible factor 1 ( HIF-1 ) to overcome any shortage of oxygen . - pubmed
Thyroid hormones have been found to augment the oxygen capacity of the blood by increasing the production of erythropoietin ( EPO ) and to improve perfusion by vasodilation through the augmented expression of adrenomedullin ( ADM ) . - pubmed
Knocking down HIF-1alpha by short interfering RNA ( siRNA ) clearly demonstrated that thyroid hormone - induced target gene expression required the presence of HIF-1 . - pubmed
Although an increased association of the two known coactivators of HIF-1 , p300 and SRC-1 , was found , thyroid hormone did not affect the activity of the isolated COOH - terminal transactivating domain of HIF-1alpha . - pubmed
Increased synthesis of HIF-1alpha may contribute to the adaptive response of increased oxygen demand under hyperthyroid conditions . - pubmed
The calcium / calmodulin - dependent protein kinases ( CaM kinases ) are a family of related kinases that are activated in response to increased intracellular calcium concentrations . - pubmed
Surprisingly , this activation occurs in the absence of any detectable calcium flux , suggesting a novel means for the activation of these kinases . - pubmed
The addition of catalase to the cultures inhibited PMA - induced activation of the CaM kinases , suggesting that similar to hydrogen peroxide , PMA also activates the CaM kinases via the production of ROI . - pubmed
In support of this concept , okadaic acid and microcystin - LR , which are inhibitors of protein phosphatase 2A ( PP2A ) , induced CaM kinase II and IV activity in these cells . - pubmed
A synthetic p38 alpha MAP kinase inhibitor , SD-282 ( 40 mg / kg ) or vehicle ( 0.25 % Tween 80 in saline ) was given intraperitoneally twice a day for 3 days , concomitant with ISO treatment . - pubmed
The ISO - induced expression of COX-2 , collagen I and III , and fibronectin genes was reduced significantly ( P & lt ; 0.05 in all cases ) by administration of SD-282 . - pubmed
Furthermore , dopamine modulated the circadian rhythms in expression of LC opsin mRNA . - pubmed
Administration of dopamine increased LC opsin mRNA expression , but only in the early morning . - pubmed
Effect of DNA polymerase beta loop variants on discrimination of O6 - methyldeoxyguanosine modification present in the nucleotide versus template substrate . - pubmed
We have examined the mechanism of DNA polymerase beta ( pol beta ) lesion discrimination using alkylated dNTP versus alkylated DNA template substrates and the pol beta variants R253 M and E249K. - pubmed
Both of these amino acid variants are located in the loop region of the palm domain and are known to play a role in pol beta fidelity and discrimination of 3 '- azido-3 '- deoxythymidine triphosphate substrates . - pubmed
Under steady - state conditions , the ratio of inherent reactivity values for the m6dGTP substrate relative to the dGTP substrate was greater for both variant polymerases than for wild - type ( WT ) pol beta . - pubmed
Using N - methyl - N - nitrosourea- ( MNU- ) modified DNA templates in the HSV - tk in vitro assay , no difference among the enzymes in the frequency of alkylation - induced G to A transition mutations was observed . - pubmed
We conclude that a specific interaction at the loop of the palm domain is involved in pol beta discrimination of the m6 G lesion when present on the incoming dNTP substrate but not when present in the DNA template . - pubmed
Neither protein kinase C- nor protein tyrosine kinase - mediated pathways were involved in regulating riboflavin uptake . - pubmed
The system appears to be regulated by protein kinase A and Ca2 +/calmodulin pathways . - pubmed
Mutant genes giving rise to high GFP fluorescence intensity indicating high levels of soluble TEV - GFP were subsequently transferred to a vector providing a C - terminal histidine tag for expression , purification , and activity tests of mutated TEV . - pubmed
We identified a mutant , TEV(SH ) , in which three amino acid substitutions result in a five - fold increase in the yield of purified protease with retained activity . - pubmed
The substrate specificity of SlrA is typical for prokaryotic and eukaryotic cyclophilins , with Suc - Ala - Ala - Pro - Phe - p - nitroanilide ( pNA ) being the most rapidly catalyzed substrate . - pubmed
Vitamin D in chronic kidney disease : a systemic role for selective vitamin D receptor activation . - pubmed
Optimization of 2,4 - diaminopyrimidines as GHS - R antagonists : side chain exploration . - pubmed
The synthesis and structure - activity relationships of the 4- and 6 - substituents of 2,4 - diaminopyrimidine - based growth hormone secretagogue receptor ( GHS - R ) antagonists are described . - pubmed
Diaminopyrimidines with 6 - norbornenyl ( 4n ) and 6 - tetrahydrofuranyl ( 4p ) substitutents were found to exhibit potent GHS - R antagonism and good selectivity ( approximately 1000 - fold ) against dihydrofolate reductase . - pubmed
To elucidate why DH , an agonist , and MSVIII-19 , a competitive antagonist , bind selectively to glutamate receptor ( GluR ) 5 but not to the KA2 KAR subunit , we used molecular dynamics simulations to generate binding models that were tested experimentally in radioligand binding and electrophysiological assays . - pubmed
Three candidate sites , Val685 , Leu735 , and Ser741 in GluR5 , corresponding to Ile669 , Phe719 , and Met725 in KA2 , were predicted to underlie the distinct binding profiles of the marine toxins . - pubmed
Most notably , mutation of Met725 to serine in KA2 increased the affinity of DH by 350 - fold ; in contrast , mutation of one or more of the residues in GluR5 did not markedly alter DH binding . - pubmed
In the present contribution , we address the problem of the nuclearity of the iron species in Fe - ZSM-5 by a combination of techniques , that is , UV - Vis , EXAFS , Magnetic Circular Dichroism ( MCD ) and magnetisation measurements . - pubmed
Propofol reduces pulmonary edema by prevention of cytokine - mediated impairment of alveolar ion and fluid transport . - pubmed
The pellet remaining after SDS extraction was also treated with trypsin . - pubmed
AIM : The aim of this study was to evaluate the maxillary sinus growth and health in the presence and absence of postnasal air flow through a unique model . - pubmed
In contrast , phospho - Akt was down - regulated to the same extent 2 h after acute AMPH or repeated AMPH with an AMPH challenge . - pubmed
These data implicate differential regulation of phospho - extracellular signal - regulated kinase , phospho - cAMP response element - binding protein versus phospho - Akt in sensitized responses to AMPH . - pubmed
PGE2 inhibits apical K channels in the CCD through activation of the MAPK pathway . - pubmed
We used the patch - clamp technique and Western blot analysis to explore the effect of PGE ( 2 ) on ROMK - like small - conductance K ( SK ) channels and Ca ( 2 +)-activated big - conductance K channels ( BK ) in the cortical collecting duct ( CCD ) . - pubmed
Application of 10 microM PGE ( 2 ) inhibited SK and BK channels in the CCD . - pubmed
Moreover , either inhibition of PKC or blocking mitogen - activated protein kinase ( MAPK ) , P38 and ERK , abolished the effect of PGE ( 2 ) on SK channels in the CCD . - pubmed
The effect of PGE ( 2 ) on SK channels was completely blocked in the presence of SC-51089 , a specific EP1 receptor antagonist , and mimicked by application of sulprostone , an agonist for EP1 and EP3 receptors . - pubmed
Application of PGE ( 2 ) significantly stimulated the phosphorylation of P38 and ERK within 5 min . - pubmed
The dose - response curve of PGE ( 2 ) effect shows that 1 , 5 , and 10 microM PGE ( 2 ) increased the phosphorylation of P38 and ERK by 20 - 21 , 50 - 80 , and 80 - 100 % , respectively . - pubmed
The stimulatory effect of PGE ( 2 ) on MAPK phosphorylation was not affected by indomethacin but abolished by inhibition of PKC . - pubmed
This suggests that the effect of PGE ( 2 ) on MAPK phosphorylation is PKC dependent . - pubmed
We conclude that PGE ( 2 ) inhibits SK and BK channels and that there is an effect of PGE ( 2 ) on SK channels in the CCD through activation of EP1 receptor and MAPK pathways . - pubmed
Also , high concentrations of PGE ( 2 ) induced by K restriction may be partially responsible for increasing MAPK activity during K restriction . - pubmed
On the basis of these analytical results , we propose a pathway for dioxane oxidation by monooxygenase - expressing cells in which dioxane is first converted to 2 - hydroxy-1,4 - dioxane , which is spontaneously oxidized to HEAA . - pubmed
( bevacizumab ) , which is reactive with all VEGF - A isoforms , was radiolabeled with In-111 or with I-125 . - pubmed
To develop potent and highly selective transthyretin ( TTR ) amyloidogenesis inhibitors , it is useful to systematically optimize the three substructural elements that compose a typical TTR kinetic stabilizer : the two aryl rings and the linker joining them . - pubmed
Coconsideration of amyloid inhibition and ex vivo plasma TTR binding selectivity data reveal that direct connection of the two aryls or linkage through nonpolar E - olefin or -CH 2CH 2- substructures generates the most potent and selective TTR amyloidogenesis inhibitors exhibiting minimal undesirable binding to the thyroid hormone nuclear receptor or the COX-1 enzyme . - pubmed
Here , we show that hippocampal CA1 pyramidal cell synapses differ in size , shape , and glutamate receptor expression depending on the laterality of presynaptic origin . - pubmed
Acromegaly : correlation between expression of somatostatin receptor subtypes and response to octreotide - lar treatment . - pubmed
About one - third of acromegalics are resistant to the clinically available somatostatin analogs ( SA ) . - pubmed
N ( G )- hydroxy - L - arginine , a putative intermediate in the NOS - reaction , gave no NO emission . - pubmed
Production of NO was decreased by anoxia or by the addition of catalase , but was increased by conditions inducing reactive oxygen ( ROS ) or by the addition of hydrogen peroxide . - pubmed
Cell - free enzyme solutions generating superoxide or hydrogen peroxide also led to the formation of NO from HA or ( with lower rates ) from SHAM , and nitrite was also an oxidation product . - pubmed
Unexpectedly , the addition of superoxide dismutase ( SOD ) to cell suspensions stimulated NO formation from hydroxylamines , and SOD alone ( without cells ) also catalysed the production of NO from HA or SHAM . - pubmed
NO production by SOD plus HA was higher in nitrogen than in air , but from SOD plus SHAM it was lower in nitrogen . - pubmed
Thus , SOD - catalysed NO formation from SHAM and from HA may involve different mechanisms . - pubmed
BACKGROUND : In this study , levels and rates of change in total testosterone ( T ) , sex hormone - binding globulin ( SHBG ) and free androgen index ( FAI ) were related to chronological age and to the final menstrual period ( FMP ) as an indicator of ovarian aging . - pubmed
Huntington ' s disease ( HD ) is an autosomal dominantly inherited disorder , caused by an expanded polyglutamine region of a protein called huntingtin . - pubmed
Their early rise in intensity , occurring within few minutes after the alcohol injection , emphasizes how susceptible troponin bands are to chemical / ischemic insults . - pubmed
The inhibitor is a stable deoxy analogue of the tetrahedral intermediate presumed to form in the NeuB reaction and was synthesized as a mixture of stereoisomers at the key tetrahedral center . - pubmed
Second , it serves as a source of nucleophilic water and delivers it to the si face of the oxocarbenium intermediate to generate a tetrahedral intermediate with a ( 2R)-configuration . - pubmed
This specificity is well explained by the very hydrophobic nature of the S1 ' subsite that consists of Tyr(189 ) , Ile(213 ) , Ile(300 ) , Met(289 ) , Val / Leu(291 ) and Leu(298 ) . - pubmed
The dual characteristics of chymosin are due to the occurrence of polar / charged residues in the S1 ' subsite , such as Glu / Asp(289 ) , Gln(298 ) and Lys / Gln(299 ) , which are different from the S1 ' subsite of pepsin A. - pubmed
We aimed to determine the value of sidestream end - tidal carbon dioxide ( SS - ETCO2 ) measurement in patients with chronic obstructive pulmonary disease ( COPD ) in the emergency department . - pubmed
We also studied correlations between Se and other biochemical variables ( age , CSF protein concentrations and glutathione peroxidase activity and plasma Se values ) . - pubmed
No association was observed between plasma and CSF Se concentrations . - pubmed
Median CSF Se values were 32 times lower when compared with those for plasma . - pubmed
In conclusion , CSF Se concentrations depend on age and total CSF protein values . - pubmed
The association observed between CSF Se and GPX activity suggests that Se quantification might be a reflection of some Se - dependent protein function . - pubmed
1 - Aminobenzotriazole ( ABT ) has been widely used in drug development process as an irreversible inhibitor of CYP enzymes . - pubmed
One potential use of ABT is to potentiate pharmacological effects of rapidly - metabolized drugs on CYP expression by inhibiting their metabolism ; however , ABT 's own effects on CYP expression have been unknown . - pubmed
In this study , we show that ABT up - regulates expression of CYP2B6 and CYP3A4 potentially by activating nuclear receptor CAR . - pubmed
ABT also modulated CYP - inducing actions of CITCO and rifampin , the known inducers of CYP2B6 and CYP3A4 . - pubmed
These results suggest that the use of ABT as a potentiator of pharmacological effects of rapidly - metabolized drugs is limited due to its own pharmacological actions on CYP expression as a CAR activator . - pubmed
The elastic biomaterial is chemically cross - linked via interaction between primary amine groups naturally present on the two proteins , silk and elastin , or chemically introduced on hyaluronan and N - succinimide functionalities of the cross - linker . - pubmed
BACKGROUND : The copper ( Cu ) transporter ( COPT / Ctr ) gene family has an important role in the maintenance of Cu homeostasis in different species . - pubmed
However , only two , COPT1 and COPT5 , have been characterized for their functions in Cu transport . - pubmed
However , these COPT genes , which showed different tissue - specific expression patterns and Cu level - regulated expression patterns , were also transcriptionally influenced by deficiency of iron , manganese , or zinc . - pubmed
Tight glucose control ( TGC ) using a sliding scale based on intermittent blood glucose measurements occasionally can have a fatal outcome as a result of insulin - induced hypoglycemia . - pubmed
The mean blood glucose level was 129.7 + - 9.7 mg / dl in the TGC group and 200.9 + - 14.7 mg / dl in the GC group ( P & lt ; 0.01 , ANOVA ) . - pubmed
Histologic analysis from group 2 showed that , compared with placebo treatment , collagen organization in avotermin - treated scars more closely resembled normal skin in 14 of 19 cases . - pubmed
We have recently performed the immune - LCM of formaldehyde - fixated , paraffin - embedded tissues immunostained with a monoclonal antibody Factor VIII . - pubmed
CD300f also binds PMA / ionomycin - activated splenocytes and Ag - stimulated T cells , an interaction inhibited by Annexin V. By ELISA , cosedimentation , and surface plasmon resonance using phospholipid - containing liposomes , we show that CD300f preferentially binds phosphatidylserine and requires a metal ion . - pubmed
We conclude that expression of CD300f conveys additional capacity to recognize phosphatidylserine to myeloid cells . - pubmed
Early graft function was defined as IGF ( N=17 ) when serum creatinine ( sCr ) was & lt;264 micromol / l at 3(rd ) postoperative day ( POD ) ; as SGF ( N=20 ) when sCr & gt;264 micromol / l and not more than 1 dialysis was performed ; and as DGF ( N=19 ) when more than 1 dialysis was done . - pubmed
The research challenge for this detector is implementing effective detection of scintillation photons by covering six faces of a segmented crystal block with silicon photomultipliers ( SiPMs ) . - pubmed
An inactive t - PA variant inhibited the t - PA - mediated increase in permeability , whereas blockade of low - density lipoprotein receptors or exposed lysine residues resulted in similar inhibition , implying a role for both a t - PA receptor , most likely a low - density lipoprotein receptor , and a plasminogen receptor . - pubmed
SNP induced nephrotoxicity by increasing serum creatinine and blood urea nitrogen , SNP significantly decreased malate dehydrogenase , glucose-6 - phosphatase , fructose-1,6 - bisphosphatase and malic enzyme but increased lactate dehydrogenase and glucose-6 - phosphate dehydrogenase . - pubmed
The activity of catalase and glutathione peroxidase decreased concomitantly with increased lipid peroxidation , indicating that the significant kidney damage has been inflicted by SNP . - pubmed
Biomarkers of oxidative protein damage ( 3 - nitrotyrosine ; 3 - NT ) and oxidative DNA damage ( 8 - oxo - deoxyguanosine ; 8 - oxo - dG ) were also assessed . - pubmed
Functional indicators of oxidative stress included relative levels of 3 - chlorotyrosine ( 3 - CT ) , an established biomarker of a chronic inflammatory response , and aconitase activity , a biomarker of mitochondrial superoxide production . - pubmed
There was a significant increase in 3 - CT levels in both brain regions ( P<0.01 ) , whereas aconitase activity was significantly decreased in autism cerebellum ( P<0.01 ) , and was negatively correlated with GSH / GSSG ( P=0.01 ) . - pubmed
Pre - exposure to copper partially prevented the mercury effect on liver but not on kidney PBG synthase , and prevented the increase of the creatinine levels . - pubmed
Here we describe in - life and post - mortem observations for a hypomorphic Ercc1 variant , Ercc1(-/Delta7 ) , which is hemizygous for a single truncated Ercc1 allele , encoding a protein lacking the last seven amino acids . - pubmed
Based on amino acid sequence homology , hydrophobic cluster and three dimensional structure analyses , it was attested that the xylanase belongs to the glycoside hydrolase ( GH ) families 10 with five carbohydrate binding domains . - pubmed
thermarum is one of the hyperthermophilic xylanases that exhibits high thermostability , and thus , is a suitable candidate for generating XOs from cellulosic materials such as agricultural and forestry residues for the uses as prebiotics and precursors for further preparation of furfural and other chemicals . - pubmed
The SCN contains the body 's master ' clock ' while the pineal - SCN nexus is intricate to the nighttime production of melatonin . - pubmed
Through its actions on melatonin receptors agomelatine can resynchronise circadian rhythms . - pubmed
Exogenous melatonin administration and bright light therapy have mixed results in limited controlled clinical evaluations . - pubmed
Furthermore , agomelatine has other actions which pre - clinical studies suggest are as important to its therapeutic effects as are its actions on melatonin receptors ipso facto its resynchronising properties . - pubmed
Maraviroc , a CCR5 antagonist , blocks interactions between the viral envelope proteins and the CCR5 coreceptor . - pubmed
Enfuvirtide , a fusion inhibitor , disrupts conformational changes in gp41 that drive membrane fusion . - pubmed
BACKGROUND : Our previous studies showed that hypothermic storage ( HS ) induces red blood cell ( RBC ) microparticle ( RMP ) generation and changes in phosphatidylserine ( PS ) and CD47 expression on RBCs and RMPs . - pubmed
Units were assessed for hemolysis , adenosine triphosphate ( ATP ) concentration , lipid composition , and RMP generation , as well as PS and CD47 expression throughout 49 days of HS . - pubmed
Synthesis and in vitro urease inhibitory activity of N , N '- disubstituted thioureas . - pubmed
Thiourea derivatives ( 1 - 38 ) were synthesized and evaluated for their urease inhibition potential . - pubmed
The synthetic compounds showed a varying degree of in vitro urease inhibition with IC50 values 5.53 + - 0.02 - 91.50 + - 0.08 muM , most of which are superior to the standard thiourea ( IC50 = 21.00 + - 0.11 muM ) . - pubmed
Optical absorption and electron paramagnetic resonance ( EPR ) spectroscopy experiments reveal that red light induces the reduction of perfluoroisopropyl - substituted zinc ( ii ) phthalocyanine ( F64PcZn ) dissolved in ethanol . - pubmed
The UV - induced triplet state of F64PcZn is characterized using a combination of transient EPR experiments and DFT computations . - pubmed
METHODS : In the present work , we further analyzed the peptidase expression in the midgut of sugar - fed females using 1DE - substrate gel zymography , two - dimensional electrophoresis ( 2DE ) , mass spectrometry ( MS ) , and protein identification based on similarity . - pubmed
Concomitant use of the fluorogenic substrate Z - Phe - Arg - AMC and trypsin - like serine protease inhibitors corroborated the zymographic findings . - pubmed
Furthermore , the use of avidin - biotin interaction allows highly selective pre- or post - functionalization of the protein crystals in a modular way with different types of functional units , such as fluorescent dyes , enzymes and plasmonic nanoparticles . - pubmed
The treatments analysed were : antihypertensive treatments , statins , antiplatelet drugs , xanthine oxidase inhibitors , correction of metabolic acidosis , treatment with ( calcium or non - calcium ) phosphate binders , vitamin D ( calcitriol or paricalcitol ) , erythropoietin and the availability of an internal arteriovenous fistula ( IAVF ) . - pubmed
In the multivariate models , we observed that , in addition to age , the comorbidity index , serum albumin , pre - dialysis treatment with angiotensin - converting - enzyme inhibitors and/or angiotensin receptor blockers , correction of acidosis with sodium bicarbonate and IAVF at the start of haemodialysis were significantly associated with better survival in dialysis . - pubmed
BACKGROUND AND OBJECTIVES : Pegylated interferon ( peg - IFN )- alpha2a and - alpha2b show different pharmacokinetic properties but are used interchangeably for hepatitis C treatment in traditional dual combinations and with newer agents . - pubmed
Histology grading and staging , interleukin-28B ( IL28B ) status and baseline viral genotype , alanine aminotransferase , gamma glutamyltransferase and glucose were analysed . - pubmed
We identified accD1 and fasA as key genes of fatty acid synthesis , encoding the beta - subunit of the acetyl - CoA carboxylase and a type - I fatty acid synthase , respectively , and observed their repression during growth on minimal medium with acetate . - pubmed
In the present work we establish by co - isolation and gel - mobility shifts oleoyl - CoA and palmitoyl - CoA as effectors of FasR , and show by DNA microarray analysis that in presence of exogeneous fatty acids accD1 and fasA are repressed . - pubmed
These results are evidence that acyl - CoA derivatives derived from extracellular fatty acids interact with FasR to repress the genes of fatty acid synthesis . - pubmed
This model also explains the observed repression of accD1 and fasA during growth on acetate , where apparently the known high intracellular acetyl - CoA concentration during growth on this substrate requires reduced accD1 and fasA expression for fine control of de novo fatty acid synthesis . - pubmed
However , the genes specific to mycolic acid synthesis , which are in part shared with linear fatty acid synthesis , are not controlled by FasR , which is in agreement with the fact that they can not be supplied from the extracellular environment but that their synthesis fully depends on a constant supply of linear fatty acid chains . - pubmed
The proportion of patients who died from cancer was the highest in the calcium channel blocker ( CCB ) group ( 6.5 % ) , followed by thiazide diuretics ( 4.4 % ) , angiotensin converting enzyme inhibitors ( 4.2 % ) and beta - blockers ( 2.6 % ) . - pubmed
Sphingosylphosphorylcholine potentiates vasoreactivity and voltage - gated Ca2 + entry via NOX1 and reactive oxygen species . - pubmed
The potentiation was greatly suppressed or abolished by inhibition of phospholipase C ( PLC ; U73122 ) , PKCepsilon ( inhibitory peptide ) , Src ( PP2 ) , and NADPH oxidase ( VAS2870 ) , and also by Tempol ( superoxide scavenger ) , but not by inhibition of Rho kinase ( Y27632 ) . - pubmed
SPC elevated reactive oxygen species ( ROS ) in vascular smooth muscle cells , and this was blocked by PP2 , VAS2870 , and siRNA knockdown of PKCepsilon . - pubmed
CONCLUSION : Our results suggest that low concentrations of SPC activate a PLC - coupled and NOX1 - mediated increase in ROS , with consequent enhancement of voltage - gated Ca2 + entry and thus vasoreactivity . - pubmed
Results showed that the HRT of 2.7 days with TM / TP = 0.57 was the best to achieve simultaneous nutrients removal for the influent with initial soluble chemical oxygen demand ( SCOD ) of about 2000 mg L(-1 ) ( only 0.55 mg L(-1 ) NH4 - N , & lt ; 0.1 mg L(-1 ) nitrate , and 0.14 mg L(-1 ) soluble phosphorus in the effluent ) . - pubmed
The diagnostic protocol consisted of 1 ) history of the reaction , 2 ) measurement of serum baseline tryptase and specific IgE - assays for latex , beta - lactams and succinylcholine , 3 ) skin tests for the agents listed in the anaesthesia chart and for others likely to be safe for future use , latex , and others medications administered during the perioperative period ( i.e. - pubmed
A diagnosis of IgE - mediated reaction was established in 55 cases ( 28.50 % ) ; the most common causes were neuromuscular blocking agents , followed by latex and beta - lactams . - pubmed
The data from 11C - PIB PET scanning showed that amyloid beta deposition in PCA was comparable to TAD . - pubmed
Population - weighted , logistic models estimated how strongly each anthropometric indicator was associated with 5 cardiometabolic disorders : Dysglycemia ( glycated hemoglobin & gt;=5.7 % ) , HyperNonHDLc ( non - high - density - lipoprotein [ HDL ] cholesterol & gt;=4.14 mmol / L , or taking anticholesteremic medications ) , Hypertension ( systolic blood pressure & gt;=140 mm Hg or diastolic blood pressure & gt;=90 mm Hg , or taking antihypertensive medications ) , HyperALT ( alanine transaminase & gt;=p75 [ 75th percentile , sex - specific ] ) , and HyperGGT ( gamma - glutamyltransferase & gt;=p75 [ sex - specific ] ) . - pubmed
Selenium Augments microRNA Directed Reprogramming of Fibroblasts to Cardiomyocytes via Nanog . - pubmed
Removal of insulin - transferrin - selenium completely inhibited the effect of reprogramming media upon cardiac gene expression and the addition of selenium to standard culture media recapitulated the effects of RM . - pubmed
Transient lymphopenia is one hallmark of sepsis , and emergent data indicate the CD4 T cell compartment in sepsis survivors is numerically and functionally altered ( when examined at the Ag - specific level ) compared with nonseptic control subjects . - pubmed
Previous data from our laboratory demonstrated Ag - independent , lymphopenia - induced homeostatic proliferation to be a contributing mechanism by which CD4 T cells numerically recover in sepsis survivors . - pubmed
However , we reasoned it is also formally possible that some CD4 T cells respond directly to Ag expressed by gut - resident microbes released during polymicrobial sepsis . - pubmed
Thus , our data demonstrate that the numerical recovery and functional responsiveness of Ag - specific CD4 T cells in sepsis survivors is , in part , modulated by the intestinal barrier 's health discreetly defined by individual bacterial populations of the host 's microbiome . - pubmed
Charged Arg61 / Arg196 and Glu80 / Glu215 on the conserved motif of Tl - gal N - terminal CRD and C - terminal CRD are critical amino acids for recognizing carbohydrate binding , and the residues can affect protein folding and structure . - pubmed
RESULTS : Our analysis revealed the lack of clinically relevant interactions between preparations of levodopa and benserazide ( used for the treatment of PD ) and angiotensin - converting enzyme inhibitors , antagonists of AT1 receptor for angiotensin II or antagonists of beta - adrenoreceptors ( beta - adrenolytics ) . - pubmed
In contrast , knockdown of PPARD using small interfering RNA dramatically decreased the expression of genes related to fatty acid activation ( ACSL1 ) and lipid formation ( PLIN2 ) and increased the expression of genes related to fatty acid transport ( FABP3 ) and triacylglycerol synthesis ( AGPAT6 and DGAT1 ) . - pubmed
The expression of genes related to fatty acid synthesis ( FASN ) , hydrolysis ( PNPLA2 ) , and fatty acid oxidation ( CPT1A ) was downregulated significantly only after knockdown of PPARD in cells incubated with GW . - pubmed
Group I ( paracetamol group ) received 15 mg / kg/6 h IV paracetamol infusion for 3 days . - pubmed
Group III ( indomethacin group ) received 0.2 mg / kg/12 h indomethacin IV infusion for three doses . - pubmed
Competition binding studies were performed using cell lysates with high SV2A expression and [ 3 H ] brivaracetam as a radioligand . - pubmed
Subsequent concentration - inhibition curves revealed the antihistaminic prodrug loratadine ( Ki = 1.16 muM ) and the antimalarial drug quinine ( Ki = 2.03 muM ) to be the most potent SV2A protein ligands of the investigated drug library . - pubmed
A pharmacophore model was established , which indicated steric and electronic conformities of brivaracetam with the new SV2A ligands , and preliminary structure - activity relationships were determined . - pubmed
The anti - convulsive effects of the natural product quinine may - at least in part - be explained by interaction with SV2A. - pubmed
The estimated 5 - year major amputation rate was lower in the DES arm ( 19.3 % versus 34.0 % for PTA - BMS ; P=0.091 ) . - pubmed
The 5 - year rates of amputation- and event - free survival ( survival free from major amputation or reintervention ) were significantly higher in the DES arm compared with PTA - BMS ( 31.8 % versus 20.4 % , P=0.043 ; and 26.2 % versus 15.3 % , P=0.041 , respectively ) . - pubmed
The aim of this study was to develop a controlled release drug - in - adhesive patch containing escitalopram ( ESP ) using ion - pair technique . - pubmed
The controlled release of ESP was attributed to the interactions among ESP - counter ion - PSA by hydrogen bonding , and counter ion enhanced the interaction between ESP and PSA molecule , which acted as a " bridge " between them . - pubmed
Using PERCIST criteria , 14 lesions ( 78 % ) showed a metabolic response in PSMA PET with a reduction of SUV of at least 30 % , as well as a significant decrease in lesion size ; in seven of these lesions , no uptake of 68Ga - PSMA was detectable . - pubmed
The expression of HbMT2b , an isoform present in latex , decreased significantly in the latex following the stress - inducing treatments of tapping , wounding and ethephon ( an ethylene generator ) . - pubmed
Inhibitors of the sodium - glucose co - transporter-2 ( SGLT-2 ) are a novel class of glucose - lowering agents that show promising results . - pubmed
Among all blood glucose lowering drugs , the proportional reporting ratio ( PRR ) was increased only for SGLT-2 inhibitors ( 5.55 [ 4.23 , 7.29 ] ) . - pubmed
While we observed an expected signal for canagliflozin ( 7.09 [ 5.25 , 9.57 ] ) , the PRR was also high for empagliflozin ( 4.96 [ 2.89 , 8.50 ] ) and , for toe amputations only , for dapagliflozin ( 2.62 [ 1.33 , 5.14 ] ) . - pubmed
Carbon dioxide conversion mediated by transition metal complexes continues to attract much attention because of its future potential utilization as a nontoxic and inexpensive C1 source for the chemical industry . - pubmed
Our research is inspired by the efficient enzymatic CO2 catalysis occurring at the active site of carbon monoxide dehydrogenase . - pubmed
YAP Tyrosine Phosphorylation and Nuclear Localization in Cholangiocarcinoma Cells Are Regulated by LCK and Independent of LATS Activity . - pubmed
YAP is known to be regulated by a serine / threonine kinase relay module ( MST1 / 2 - LATS1 / 2 ) culminating in phosphorylation of YAP at Serine 127 and cytoplasmic sequestration . - pubmed
However , YAP also undergoes tyrosine phosphorylation , and the role of tyrosine phosphorylation in YAP regulation remains unclear . - pubmed
YAP was phosphorylated on tyrosine 357 ( Y357 ) in CCA cell lines and PDX models . - pubmed
SRC family kinase ( SFK ) inhibition with dasatinib resulted in loss of YAPY357 phosphorylation , promoted its translocation from the nucleus to the cytoplasm , and reduced YAP target gene expression , including cell lines expressing a LATS1/2 - resistant YAP mutant in which all serine residues were mutated to alanine . - pubmed
Finally , dasatinib displayed therapeutic efficacy in PDX models . - pubmed
Members of the Copper Metabolism Murr1 Domain containing ( COMMD ) family of 10 proteins are known to interact with ENaC. - pubmed
Specifically , we review the role of endovascular therapy in the prevention of post - thrombotic syndrome following acute lower limb deep vein thrombosis ( DVT ) ( ATTRACT trial ) , the efficacy of edoxaban as the first direct oral anticoagulant used for the treatment of cancer - associated thrombosis ( HOKUSAI VTE - Cancer study ) , whether aspirin can be considered for thromboprophylaxis post - major orthopedic surgery ( EPCAT-2 trial ) , and the need for anticoagulant therapy for treatment of isolated distal DVT ( CACTUS trial ) . - pubmed
Citric Acid Capped CdS Quantum Dots for Fluorescence Detection of Copper Ions ( II ) in Aqueous Solution . - pubmed
This transfer resulted in a blue shift of the excitonic photoluminescence ( PL ) peak generated by MoS2 monolayers , together with an intensity increase . - pubmed
This suspension technique also allowed the observation of PL resulting from interlayer excitons in the MoS2 / WS2 vdW heterostructures . - pubmed
SA / CdS-2 ( mass ratio SA : CdS = 1:2 ) exhibited the highest reduction efficiency of Cr ( VI ) , which could reduce the concentration of Cr ( VI ) to 0 mg / L after 60 min illumination . - pubmed
Morphine , which acts through opioid receptors , is one of the most efficient analgesics for the alleviation of severe pain . - pubmed
On the bases of the in vitro biological activities of the hybrids , two analogs , opioid agonist / NK1 antagonist Tyr -[ d - Lys - Phe - Phe - Asp ]- Asn - d - Trp - Phe - d - Trp - Leu - Nle - NH2 ( 2 ) and opioid agonist / NK1 agonist Tyr -[ d - Lys - Phe - Phe - Asp ]- Gln - Phe - Phe - Gly - Leu - Met - NH2 ( 4 ) , were selected for in vivo tests . - pubmed
When the analysis was restricted to those who sustained remission , exposure to olanzapine compared with placebo was associated with significant decreases in cortical thickness in the left hemisphere ( beta [ SE ] , 0.04 [ 0.009 ] ; t34.4 = 4.7 ; P & lt;.001 ) , and the right hemisphere ( beta [ SE ] , 0.03 [ 0.009 ] ; t35.1 = 3.6 ; P & lt;.001 ) . - pubmed
Switchable presentation of cytokines on electroactive polypyrrole surfaces for hematopoietic stem and progenitor cells . - pubmed
By a stepwise Fourier transform infrared spectroscopy , the reaction between MXene and NBR was proved . - pubmed
At only 2.8 vol% MXene , a swelling ratio of 1.61 was achieved , representing a 75 % reduction compared to NBR / reduced graphene oxide and carbon nanotube nanocomposites containing the same filler fraction . - pubmed
Overall , emulsion compounding proved to be an efficient technique for forming NBR / MXene nanocomposites . - pubmed
The revealed reaction between MXene and NBR to create functional polymer nanocomposites would provide a platform for utilising MXene in other polymers . - pubmed
Combination antiviral therapy with lopinavir / ritonavir , arbidol and interferon - alpha1b for COVID-19 . - pubmed
A dual - combination of ribavirin and interferon - alpha has been the widely used regimen for the treatment of this disease in China . - pubmed
Herein , we report a semiconductive , proton - conductive , microporous hydrogen - bonded organic framework ( HOF ) derived from phenylphosphonic acid and 5,10,15,20 - tetrakis [ p - phenylphosphonic acid ] porphyrin ( GTUB5 ) . - pubmed
This study aimed to determine the relation of best corrected visual acuity ( BCVA ) and low luminance visual acuity ( LLVA ) in proliferative diabetic retinopathy ( PDR ) following treatment with either aflibercept or pan - retinal photocoagulation ( PRP ) . - pubmed
Post aflibercept therapy , the mean change in BCVA and LLVA at 52 weeks after aflibercept was +2.1 ( SD 6.05 ) letters and +0.39 ( SD 5.6 ) letters , respectively . - pubmed
The principal metabolite was ( R )-(-)- 2 - phenylhydantoic acid , formed stereo - specifically in a ring - opening reaction of ( R )- 5 - phyenylhydantoin by dihydropyrimidinase ( EC 3.5.2.2 ) . - pubmed
Exogenous cyclic AMP as well as positive effectors of the adenylyl cyclase system were able to overcome the phosphate suppression . - pubmed
The change in enzyme activity as a result of the enzyme - haptene - antibody reaction is proportional to the concentration of the haptene . - pubmed
In this modified method , cortisol was released from its binding globulin by enzymatic degradation rather than by extraction with ether , and a preincubated mixture of first and second antibody was used to separate antibody - bound cortisol from free . - pubmed
Despite the recognition of a traS + ( Slo+ ) component of DNA within that encoding the Slo+ and Sfx+ phenotypes , this gene seems nevertheless to be responsible for the Sfx+ phenotype since hydroxylamine - induced Slo- mutants of pVS438 are usually also Sfx- . - pubmed
Therefore , we quantitated the tryptophan metabolites , L - kynurenine and quinolinic acid , and we measured neopterin , a marker of interferon - gamma ( IFN - gamma ) , in blood obtained during the acute phase of each syndrome . - pubmed
Our data suggest that indoleamine-2,3 - dioxygenase ( IDO ) , the rate limiting enzyme of the kynurenine pathway of L - tryptophan metabolism , was activated in both syndromes by cytokines including IFN - gamma , and that perhaps products of tryptophan metabolism played a role in the pathogenesis of EMS and TOS . - pubmed
Some alternative approaches , including meta - regression techniques , are discussed , and illustrated with reference to examples of meta - analyses of studies of breast cancer risk following oral contraceptive use and of patterns of post - bereavement mortality . - pubmed
Protein kinase A in the absence of ATP had no effect on channel activity ( n = 3 ) . - pubmed
We tested the effect of two potent inhibitors of protein phosphatases , calyculin A and okadaic acid . - pubmed
Calyculin A appeared to be less effective in increasing open probability than okadaic acid , suggesting that the phosphatase involved is neither type 1 , 2A , nor 2B. - pubmed
Calyculin A in the absence of protein kinase A was ineffective . - pubmed
Study I : The impact of metoprolol CR / ZOK 50 mg once daily for 4 weeks compared with placebo 24 hours post dosing was to produce a significant reduction in supine ( P = .0001 ) , and standing ( P less than .0001 ) systolic blood pressures and the standing diastolic pressure ( P = .035 ) . - pubmed
The supine diastolic pressure was lower after metoprolol CR / ZOK but not significantly so . - pubmed
Study II : Metoprolol CR / ZOK 50 mg daily given to elderly hypertensives when compared with placebo 24 hours post dosing produced a significant fall in supine ( P = .022 ) and standing ( P = .022 ) diastolic pressure . - pubmed
Fixed schistosomula coated with FPLC - purified IgE and IgG gave 7.6 + /- 0.4 and 6.0 + /- 0.1 pmoles of LTC4 per 10(6 ) low - density eosinophils , respectively . - pubmed
Following preincubation with platelet - activating factor ( PAF ) ( 10(-7 ) M ) and leukotriene B4 ( LTB4 ) ( 10(-7 ) M ) , normal density eosinophils released LTC4 when in contact with larvae coated with antigen - specific IgE. - pubmed
The synthetic chemotactic tripeptide formyl - methionyl - leucyl - phenylalanine ( FMLP ) had no influence on IgE - dependent release of LTC4 from eosinophils . - pubmed
In contrast , FMLP ( 10(-7 ) M ) enhanced the IgG - dependent LTC4 release , with PAF and LTB4 also showing a small enhancing effect . - pubmed
Baseline values and post - DDAVP increase were not significantly different from our normal controls . - pubmed
The specificity of the anti - Au ( 1 ) serum used in this study was ascertained by immunodiffusion and immunoelectrophoresis , and by immunoelectron microscopic studies of the antigen - antibody complex prepared in vitro by mixing the ferritin - conjugated anti - Au ( 1 ) reagent with the purified Au ( 1 ) particles . - pubmed
Kinetic studies and characteristics of a modifier of Ca2 + -stimulated ATPase . - pubmed
The thermostable modifier increases the Mg2 + -stimulated ATPase with a parallel decrease of Ca2 + -stimulated ATPase . - pubmed
The modifier inhibited Ca2 + -stimulated ATPase by diminishing the velocity without any significant effect on the apparent affinity of binding of Ca2 + . - pubmed
The inhibition of Ca2 + -stimulated ATPase was independent of Ca2 + concentration and could not be reversed by increasing Ca2 + levels . - pubmed
Monovalent cations and calmodulin increased the Mg2 + -stimulated ATPase in the presence of the modifier . - pubmed
Thus , H-2 antigens are not only recognized by allogenic lymphocytes leading to generation of cytotoxic T lymphocytes ( CTLs ) , but it has also been demonstrated that in syngeneic systems most T cells are only able to recognize foreign antigens in conjunction with their own MHC ( major histocompatibility complex ) antigens . - pubmed
The deduced amino acid sequence of TUB2 had a high degree of homology to other fungal beta - tubulins . - pubmed
The urinary excretion of endogenous creatinine ( CRTN ) and 3 - methylhistidine ( 3 - MH ) has been proposed as a predictor of fat - free mass ( FFM ) in healthy subjects . - pubmed
CRTN excretion was found well associated with FFM ( R2 = 0.89 ; P & lt ; 0.0001 ) , whereas 3 - MH was lightly associated with FFM ( R2 = 0.33 ; P & lt ; 0.01 ) . - pubmed
Residuals from the regression of 3 - MH vs. FFM were found to be correlated with CRTN excretion and FFM ( R2 = 0.57 and 0.67 , respectively ) , suggesting that muscularity and the absolute amount of lean mass are relevant for the error in predicting FFM from 3 - MH excretion . - pubmed
Data also suggest that the relationship between 3 - MH and FFM is complex , limiting the possibility of predicting body composition from the excretion of this metabolite . - pubmed
Down - modulation of interleukin-6 gene expression by 17 beta - estradiol in the absence of high affinity DNA binding by the estrogen receptor . - pubmed
The mechanism of repression of the interleukin-6 ( IL-6 ) promoter by 17 beta - estradiol ( E2 ) was investigated in cells transfected with wild - type ( wt ) or mutant estrogen receptor ( ER ) expression vectors . - pubmed
This was in contrast to the absolute dependence of ER on its own DNA binding domain for activation of typical estrogen response element - containing promoters , as reported previously by other investigators . - pubmed
Furthermore , the repression of the IL-6 promoter by a combination of ER and E2 , unlike activation of estrogen response elements by the same combination , did not appear to be mediated via high affinity binding of the receptor to the promoter . - pubmed
Observations on the HDL system in these two families substantiate the role of cholesteryl ester / triglyceride exchange between HDL and lower - density lipoproteins in the remodelling of HDL in plasma . - pubmed
Analysis of variance using a linear model demonstrated no significant intergroup differences in O2- production by lymphokine - activated monocytes from G2 pigs . - pubmed
Litter contributed significantly to variation in monocyte O2- production in G2 pigs ( P & lt ; or = 0.005 ) and SLA - DQ ( P & lt ; or = 0.01 ) expression . - pubmed
Cyclic sulfolanes as novel and high affinity P2 ligands for HIV-1 protease inhibitors . - pubmed
The level of LSP1 RNA in tumors developing & lt;110 days after injection of MNU was 19.1 % + /- 5.2 ( mean + /- SEM ) , while the level of LSP1 in later tumors was 78.4 % + /- 13.0 ( P = 0.004 ) . - pubmed
Third , they show that there are two classes of T - lymphoma , which differ in their expression of LSP1 RNA and that the down - regulation of LSP1 is specifically associated with the early appearance of T - lymphoma after injection with MNU . - pubmed
Of the specific inhibitors of iNOS in infarcted heart muscle , S - methylisothiourea ( SMT ) was the most potent . - pubmed
Dexamethasone and cyclosporin A failed to inhibit iNOS . - pubmed
Two H3 receptor agonists , R -(-)- alpha - methylhistamine and imetit , inhibited the electrically induced increase in diastolic blood pressure in a dose - dependent manner . - pubmed
Two H3 receptor antagonists , thioperamide 1 mumol / kg and clobenpropit 0.1 mumol / kg , attenuated the inhibitory effect of imetit . - pubmed
The neurogenic vasopressor response was increased by about 15 % by thioperamide 1 mumol / kg and clobenpropit 0.1 mumol / kg and decreased by 25 % by the histamine methyltransferase inhibitor metoprine 37 mumol / kg . - pubmed
Metoprine had only a very low affinity for H3 binding sites ( labelled by 3H - N alpha - methylhistamine ; pKi 4.46 ) . - pubmed
Moreover , it demonstrates that these H3 receptors are probably activated by endogenous histamine and appear to be operative in hypertension . - pubmed
In pectate lyases , this cleft is rich in charged residues and contains an elongated ribbon of positive potential when Ca2 + is bound . - pubmed
Pectin lyase A does not bind Ca2 + but an arginine residue is found in an equivalent position to the Ca2 + ion in pectate lyase , suggesting a similar role in catalysis . - pubmed
The AsD2 radical was generated in a free - space cell by dc - glow discharge of a gaseous mixture of D2 and He over arsenic powder . - pubmed
The harmonic force field was used to calculate vibrational corrections to the rotational constants of both species and thus the zero - point average structures for both species were derived ; AsH2 : rz(AsH ) = 1.53436(15 ) A , thetaz(HAsH ) = 90.695(20 ) degrees , and AsD2 : rz(AsD ) = 1.52902(13 ) A , thetaz(DAsD ) = 90.722(17 ) degrees , where the errors in parentheses are due to residual inertial defects of the corrected moments of inertia . - pubmed
From the difference between the rz structures for AsH2 and AsD2 the re structure of AsH2 is estimated to be re(AsH ) = 1.5158(6 ) A , thetae(HAsH ) = 90.79(8 ) degrees . - pubmed
administration of CA - SLN and a camptothecin control solution ( CA - Sol ) . - pubmed
Neutrophil - induced changes in the biomechanical properties of endothelial cells : roles of ICAM-1 and reactive oxygen species . - pubmed
This study evaluated the changes in the biomechanical properties of endothelial cells ( ECs ) induced by neutrophil adhesion and the roles of ICAM-1 and reactive oxygen species ( ROS ) in modulating these changes . - pubmed
The neutrophil - induced increase in the apparent stiffness of ECs was inhibited with 1 % DMSO ( a hydroxyl radical scavenger ) , allopurinol ( a xanthine oxidase inhibitor ) , or deferoxamine ( an iron chelator ) , suggesting that ROS may be involved in mediating the EC stiffening response . - pubmed
This ROS production in ECs was completely prevented by the anti - ICAM-1 Ab and partially inhibited by allopurinol . - pubmed
Full nucleotide and amino acid sequences were considered as well as abridged sequences from the hypervariable peptide binding region , known to interact in vivo , with HLA presented foreign peptide . - pubmed
HLA - A alleles ( n = 60 ) assorted into 12 groups based on full nucleotide and amino acid sequence which with few exceptions recapitulated serological groupings , however the patterns were largely discordant with clusters prescribed by PBR sequences . - pubmed
Expression of CD16b , a low affinity Fcgamma receptor , was established by either transfection or spontaneous insertion via its glycosylphosphatidylinositol anchor . - pubmed
Calcium - sensitive potassium channel inhibitors antagonize genistein- and daidzein - induced arterial relaxation in vitro . - pubmed
The method , based on the precipitation of amine - oxidase - generated H2O2 by CeCl3 , was shown to be specific for DAO and PAO and permitted their localization in plant tissues with a high degree of resolution . - pubmed
The oxidases could provide H2O2 for peroxidase - mediated cross - linking reactions in the cell wall and may , in this capacity , play a role in the regulation of plant growth . - pubmed
The kinetics of human polymerase beta ( pol beta ) binding to the single - stranded DNA , in the ( pol beta)(16 ) and ( pol beta)(5 ) binding modes , that differ in the number of occluded nucleotide residues in the protein - DNA complexes , have been examined , using the fluorescence stopped - flow technique . - pubmed
Their morning fasting urine were collected for measurement of iodide concentration , and their sera were measured for thyroid - stimulating hormone(TSH , the third generation ) , thyroid peroxidase antibody ( TPOAb ) , thyroglobulin antibody ( TGAb ) and thyroglobulin ( TG ) . - pubmed
RESULTS : The median level of urinary iodide of the sample subjects was 614.61 micrograms / L. The prevalence of clinical and subclinical hyperthyroidism was 1.21 % and 1.12 % respectively ; 92.3 % of the clinical hyperthyroidism were of Graves ' disease and 75 % of the subjects with subclinical hypothyroidism were TRAb positive . - pubmed
Fracture characteristics of carbon fibre , ceramic and non - palladium endodontic post systems at monotonously increasing loads . - pubmed
A carbon fibre post system , three non - palladium and one palladium metal post systems , two ceramic post systems , and a metal post system with a ceramic core were studied in vitro . - pubmed
The highest mean fracture loads were found for the carbon fibre post system ( 312.5 + /- 58.8 N ) . - pubmed
The fracture load of non - palladium metal posts ( 242.3 - 300.4 N ) did not differ significantly from that of the Perma - dor post ( 265.9 N ) , which does contain palladium . - pubmed
Values of 300.3 + /- 89.3 N ( aluminium oxide ceramics ) and 193.5 + /-57.0 N ( zirconia ceramics ) were found for the ceramic posts . - pubmed
The genomic DNA was amplified by polymerase chain reaction ( PCR ) and the products were separated on 1.5 % agarose gel and visualized by ultraviolet transillumination . - pubmed
Furthermore , lowering adenosine levels or antagonizing adenosine receptors can reverse pulmonary phenotypes in this model , suggesting that chronic adenosine elevations can affect signaling pathways that mediate aspects of chronic lung disease . - pubmed
Carbodiimide may form intrahelical and interhelical crosslinks within or between tropocollagen molecules , whereas genipin may further introduce intermicrofibrillar crosslinks between adjacent collagen microfibrils . - pubmed
The present study has shown that this inhibitory profile , referred to as bacteriocin producer ( P )- type 777 activity , is due to the type A1 lantibiotic streptin . - pubmed
The fully processed form of streptin ( streptin 1 ) is a 23 - amino - acid peptide with a mass of 2,424 Da . - pubmed
However , several PAHs enhanced histamine release and IL-4 production in response to crosslinking the high - affinity IgE receptor , Fc epsilon RI . - pubmed
The enhancement seen with 1,6 - BaP - quinone involved an increase in tyrosine phosphorylation in several different substrates , including the Fc epsilon RI - associated tyrosine kinase , Lyn , and elevated reactive oxygen species ( ROS ) levels detected by dichlorofluorescein fluorescence and flow cytometry . - pubmed
Effects of different acid and sodium hypochlorite treatments on dentin collagen : a FEISEM analysis . - pubmed
Plasma RLP - cholesterol was measured by an immunoabsorption enzyme method . - pubmed
The analytical procedures employed would also have detected ethylene glycol dinitrate ( EGDN ) if present at levels greater than 2 ng , nitrobenzene ( NB ) if present at levels greater than 50 ng , mononitrotoluenes if present at levels greater than 50 ng , and the other common isomers of dinitrotoluene if these had been present at levels in excess of 10 ng . - pubmed
The relatively high volatility of EGDN , NB , and the mononitrotoluenes would , however , cause traces of these compounds to disperse rapidly . - pubmed
The cystic fibrosis ( CF ) transmembrane conductance regulator ( CFTR ) plays a crucial role in mediating duodenal bicarbonate ( HCO ( 3 )(- ) ) secretion ( DBS ) . - pubmed
To explain this apparent paradox , we hypothesized that CFTR dysfunction increases cellular [ HCO ( 3 )(- ) ] and buffering power . - pubmed
DBS was measured in situ using a standard loop perfusion model with a pH stat under isoflurane anesthesia . - pubmed
Acid - induced DBS was abolished at 1 and 3 h and was reduced 24 h after treatment with CFTR(inh)-172 , although basal DBS was increased at 24 h. CFTR(inh)-172 treatment had no effect on gastric acid or HCO ( 3 )(- ) secretion . - pubmed
Several studies have demonstrated the existence of an autonomous intranuclear phospho - inositide cycle that involves the activation of nuclear PI - PLC and the generation of diacylglycerol ( DG ) within the nucleus . - pubmed
Rates of hypertension , high low density lipoprotein ( LDL ) cholesterol , and diabetes mellitus awareness , treatment , and control for persons with a history of myocardial infarction and stroke were compared by using two nationally representative samples of the US population : the Third National Health and Nutrition Examination Survey in 1988 - 1994 ( n = 1,004 ) and the National Health and Nutrition Examination Survey in 1999 - 2002 ( n = 512 ) . - pubmed
Among such survivors , awareness of a previous diagnosis of hypertension and prevalence of self - reported diabetes mellitus remained stable while awareness of high LDL cholesterol increased from 43.3 % to 60.2 % ( p & lt ; 0.01 ) . - pubmed
Among those aware of their diagnosis , pharmacologic treatment for high LDL cholesterol increased from 33.1 % to 78.4 % and pharmacologic treatment for diabetes mellitus increased from 80.0 % to 93.6 % during this time ( each p & lt ; 0.01 ) , while pharmacologic treatment for hypertension increased nonsignificantly . - pubmed
Among those receiving pharmacologic treatment , hypertension and high LDL cholesterol control increased from 48.9 % to 59.3 % ( p = 0.05 ) and from 5.1 % to 33.1 % ( p & lt ; 0.01 ) , respectively . - pubmed
The number of US myocardial infarction and stroke survivors increased between 1988 - 1994 and 1999 - 2002 , and substantial improvements occurred in the awareness , treatment , and control of high LDL cholesterol in this population . - pubmed
This article examines the rationale for the use of the oral dual endothelin receptor antagonist bosentan in this life - threatening condition . - pubmed
Interaction of riluzole with the closed inactivated state of Kv4.3 channels . - pubmed
The effect of riluzole on Kv4.3 was examined using the whole - cell patch - clamp technique . - pubmed
Riluzole inhibited the peak amplitude of Kv4.3 in a reversible , concentration - dependent manner with an IC(50 ) of 115.6 microM. - pubmed
tau ( f ) was not altered by riluzole at concentrations up to 100 microM , but tau(s ) became slower with increasing riluzole concentration , resulting in the crossover of the currents . - pubmed
Riluzole shifted the voltage dependence of the steady - state inactivation of Kv4.3 in the hyperpolarizing direction in a concentration - dependent manner . - pubmed
The K(i ) for riluzole for interacting with the inactivated state of Kv4.3 was estimated from the concentration - dependent shift in the steady - state inactivation curve and was determined to be 1.2 muM. - pubmed
Riluzole induced a significant use - dependent inhibition of Kv4.3 . - pubmed
These results suggest that riluzole inhibits Kv4.3 by binding to the closed inactivated state of the channels and that the unbinding of riluzole occurs from the closed state during depolarization . - pubmed
Mitochondrial complex II ( succinate dehydrogenase [ SDH ] ) is part of the tricarboxylic acid cycle and the respiratory electron transport chain . - pubmed
A functionally conserved basic residue in glutathione transferases interacts with the glycine moiety of glutathione and is pivotal for enzyme catalysis . - pubmed
The present study characterized conserved residues in a GST ( glutathione transferase ) in the active - site region that interacts with glutathione . - pubmed
In the GSTD ( Delta class GST ) studied , adGSTD4 - 4 , the network consisted of His(38 ) , Met(39 ) , Asn(47 ) , Gln(49 ) , His(50 ) and Cys(51 ) . - pubmed
CONCLUSION : The targeted magnetic nanoparticles containing 5 - FU can improve the chemotherapeutic effect of 5 - FU against hepatocellular carcinoma by decreasing the expression of bcl-2 gene , and increasing the expression of bax and caspase 3 genes . - pubmed
We prospectively examined the relationship between fructose intake and incident kidney stones in the Nurses ' Health Study I ( NHS I ) ( 93,730 older women ) , the Nurses ' Health Study II ( NHS II ) ( 101,824 younger women ) , and the Health Professionals Follow - up Study ( 45,984 men ) . - pubmed
Sequence analysis showed a single nucleotide deletion in the 89.6 env gene of the mutant that caused a frame shift and truncation of 115 amino acids from the cytoplasmic domain . - pubmed
Although a shift from fatty acids ( FAs ) to carbohydrates ( CHOs ) is considered beneficial for the diseased heart , it is unclear why subjects with FA beta - oxidation defects are prone to cardiac decompensation under stress conditions . - pubmed
Carbon-13 methodology was used to assess substrate flux through energy - yielding pathways in hearts perfused ex vivo at two workloads with a physiological substrate mixture mimicking the fed state , and real - time RT - quantitative polymerase chain reaction was used to document the expression of selected metabolic genes . - pubmed
At the metabolic level , beyond the expected shift from FA ( 5 - fold down ) to CHO ( 1.5 - fold up ; P & lt ; 0.001 ) at both preloads , PPARalpha null hearts also displayed 1 ) a significantly greater contribution of exogenous lactate and glucose and/or glycogen ( 2 - fold up ) to endogenous pyruvate formation , whereas that of exogenous pyruvate remained unchanged and 2 ) marginal alterations in citric acid cycle - related parameters . - pubmed
Collectively , our data highlight the importance of metabolic alterations in CHO metabolism associated with FA oxidation defects as a factor that may predispose the heart to decompensation under stress conditions even in the fed state . - pubmed
Label - free calcium imaging in ischemic retinal tissue by TOF - SIMS . - pubmed
Thus , the TOF - SIMS technique provides a tool for the study of intercellular communication by Ca ( 2 + ) ion movement . - pubmed
A combined chemotherapy regimen comprising fludarabine , cytosine arabinoside , and granulocyte colony - stimulating factor ( FLAG ) has been used in the treatment of relapsed or refractory leukemias . - pubmed
Germination of the two genotypes was differentially sensitive to abscisic acid ( ABA ) and gibberellin ( GA ) at elevated temperatures . - pubmed
Expression of many genes involved in ABA , GA , and ethylene biosynthesis , metabolism , and response was differentially affected by high temperature and light in the two genotypes . - pubmed
ATH1 , and NTH1 hydrolyze trehalose to glucose to provide energy and assist in recovery from stress . - pubmed
ATH1Delta and NTH2Delta showed very low neutral trehalase activity and NTH1Delta did not show any neutral trehalase activity , and trehalose concentrations were higher . - pubmed
Increased neutral trehalase activity ( equivalent to the wild type ) , reduction of trehalose content and brisk sensitivity to stressors were noted in TREH - ATH1Delta strain , but not in TREH - NTH1Delta or -NTH2Delta . - pubmed
Our results suggest that TREH acts as a stress - response protein in the kidney rather than involved in utilization of exogenous trehalose . - pubmed
BACKGROUND INFORMATION : The TSPO ( 18 kDa translocator protein ) is a mitochondrial transmembrane protein involved in cholesterol transport in organs that synthesize steroids and bile salts . - pubmed
Different natural and synthetic high - affinity TSPO ligands have been characterized through their ability to stimulate cholesterol transport , but also to stimulate other physiological functions including cell proliferation , apoptosis and calcium - dependent transepithelial ion secretion . - pubmed
Enterocyte cytoplasm also contained the endogenous TSPO ligand , polypeptide DBI ( diazepam - binding inhibitor ) . - pubmed
Whereas intestinal TSPO had high affinity for the synthetic ligand PK 11195 , the pharmacological profile of TSPO in the duodenum was distinct from the jejunum and ileum . - pubmed
Specifically , benzodiazepine Ro5 - 4864 and protoporphyrin IX showed 5 - 13 - fold lower affinity for duodenal TSPO . - pubmed
CONCLUSIONS : The functional differences in subpopulations of TSPO in different regions of the intestine could be related to structural organization of mitochondrial protein complexes that mediate the ability of TSPO to modulate either chloride secretion or absorption in the duodenum and ileum respectively . - pubmed
MYB134 - overexpressing plants exhibited a concomitant reduction in phenolic glycoside concentrations and other minor alterations to levels of small phenylpropanoid metabolites . - pubmed
Our investigation of Ca ( 2 +)-signaling pathways involved in axon formation using cultured hippocampal neurons demonstrates a role for Ca ( 2 +)/ calmodulin kinase kinase ( CaMKK ) and its downstream target Ca ( 2 +)/ calmodulin kinase I ( CaMKI ) . - pubmed
These results are consistent with a model in which highly localized calcium influx through the TRPC5 channels activates CaMKK and CaMKIgamma , which subsequently promote axon formation . - pubmed
The induction of FLT3 in CML cells attenuated imatinib - induced apoptosis . - pubmed
These findings indicate that FLT3 is associated with disease progression , despite imatinib therapy . - pubmed
A prominent defense against the oxidant burden caused by CS is the glutathione ( GSH ) adaptive response in the lung epithelial lining fluid ( ELF ) and tissue . - pubmed
OBJECTIVES : Examine the effect of aging on the GSH adaptive response to CS and resulting lung sensitization to inflammation and oxidation . - pubmed
Study on nucleic acid ( CT - DNA and yeast tRNA ) binding behaviors and cytotoxic properties of a heterodinuclear Ru ( II )- Co ( III ) polypyridyl complex . - pubmed
PURPOSE : To investigate the effect of Colloidal Platinum Nanoparticles ( CPN ) on the bond strength between dentin and 4 - META / MMA - TBB resin using different concentrations of CPN . - pubmed
One control and four experimental groups ( 2 specimens per group ) were used as follows : a ) dentin surfaces treated with 10 - 3 solution , followed by rinsing with water and subsequently an acrylic rod bonded with hand - mixed 4META / MMA - TBB resin ( Super - Bond C&B , Sun Medical ) ( control ) ; b ) dentin surfaces treated with 10 - 3 etching solution , followed by rinsing with water and application of CPN ( 100 % or 10 % ) as a primer solution for 60 s and rinsed with water for 20 s , then an acrylic rod bonded with Super - Bond C&B(Etch - CPN [ 100 % or 10 % ] ) ; c ) dentin surfaces treated with CPN ( 100 % or 10 % ) for 60 s , rinsed with water for 20 s , followed by application of 10 - 3 solution , then an acrylic rod bonded with Super - Bond C&B ( CPN - Etch [ 100 % or 10 % ] ) . - pubmed
RESULTS : Etch - CPN ( 100 ) , CPN - Etch(100 ) and CPN - Etch ( 10 ) showed significantly higher bond strengths compared to the control . - pubmed
The bond strength of 4META / MMA - TBB resin was approximately doubled by CPN application . - pubmed
As compared with the model group , the abnormal changes of TIMP-1 and MMP1 expressions were ameliorated in the two groups treated either by BHR or fosinopril ( P & lt ; 0.05 ) . - pubmed
Translational characterization of [ 11C ] GSK931145 , a PET ligand for the glycine transporter type 1 . - pubmed
Although all gels became degraded in the presence of lysozyme , their degradation rate greatly depended on the genipin load . - pubmed
After extraction and separation of hemoglobin from whole blood , the polyacrylamide gel electrophoresis ( SDS - PAGE ) , cellulose acetate electrophoresis , and isoelectric focusing ( IEF ) were used to confirm Hb variabilities in these fishes . - pubmed
UV - vis spectrophotometery was also utilized to measure oxygen affinity of Hbs by sodium dithionite . - pubmed
Oxygen affinities of these Hbs were compared using Hb - oxygen dissociation curves . - pubmed
Together , these results demonstrate a significant relationship between oxygen affinity of fish hemoglobins and environmental partial pressure of oxygen . - pubmed
A case of wear and entrapment of a polyethylene disc observed in a Starr - Edwards ( SE ) mitral caged - disc valve at 37 years after implantation is reported . - pubmed
Cinefluoroscopy showed the polyethylene disc of the SE valve to have impinged against a calcified mass on the left ventricular posterior wall , causing a tipping motion of the disc during opening . - pubmed
roseus WRKY transcription factor , CrWRKY1 , that is preferentially expressed in roots and induced by the phytohormones jasmonate , gibberellic acid , and ethylene . - pubmed
Up - regulation of TDC increased TDC activity , tryptamine concentration , and resistance to 4 - methyl tryptophan inhibition of CrWRKY1 hairy roots . - pubmed
The enzyme contains 1 mol Zn ( 2 + ) in its active site and shows a unique zinc dependence . - pubmed
The fact that dehydration of d - threonine and d - allo - threonine , also catalyzed by DsdSC , is likewise Zn ( 2 + ) dependent indicates that Zn ( 2 + ) is indispensable for the elimination of hydroxyl group , regardless of the stereochemistry of C(beta ) . - pubmed
Removal of Zn ( 2 + ) results in a less polar active site without changing the gross conformation of DsdSC . - pubmed
Mutation of Cys400 or His398 within the Zn ( 2 + ) binding sites to Ala endowed DsdSC with similar properties to those of the Zn ( 2 + ) -depleted wild - type enzyme : the mutants lost the reactivity toward d - serine and d - threonine but retained that toward beta - Cl - d - alanine . - pubmed
Our data suggest that DsdSC catalyzes the alpha - hydrogen abstraction and hydroxyl group elimination in a concerted fashion . - pubmed
LRH-1 plays important roles in embryonic development , steroidogenesis and cholesterol homeostasis . - pubmed
GREB1 is a well characterised ERalpha target gene , with three estrogen response elements ( ERE ) located on its promoter . - pubmed
In the presence of ICI 182,780 , an ERalpha antagonist , LRH-1 still induced proliferation . - pubmed
Utility of FDG PET / CT in the assessment of myeloid sarcoma . - pubmed
CONCLUSION : This article highlights the utility of ( 18 ) F - FDG PET / CT , which has high sensitivity in detecting early MS and provides a systemic overview of tumor burden , and its potential role in monitoring of treatment response . - pubmed
In this article , various approaches are explored towards a water compatible MIP for the target molecule l - nicotine . - pubmed
In this study , we synthesized a novel synthetic alpha - lipoic acid - decursinol ( ALA - DA ) hybrid compound , and compared neuroprotective effects of ALA , DA or ALA - DA against ischemic damage in the gerbil hippocampal CA1 region induced by 5 min of transient cerebral ischemia . - pubmed
However , 20 mg / kg ALA - DA pre - treatment protected pyramidal neurons from ischemic damage in the CA1 region . - pubmed
In addition , 20 mg / kg ALA - DA pre - treatment markedly decreased the activation of astrocytes and microglia in the CA1 region 4 days after ischemic injury . - pubmed
Deamidation of N - terminal Gln by the Ntaq1 Nt ( Q )- amidase is a part of the Arg / N - end rule pathway , a ubiquitin - dependent proteolytic system . - pubmed
Here we identify Gln - Usp1(Ct ) , the C - terminal fragment of the autocleaved Usp1 deubiquitylase , as the first physiological Arg / N - end rule substrate that is targeted for degradation through deamidation of N - terminal Gln . - pubmed
The autocleaved Usp1 remains a deubiquitylase because its fragments remain associated with Uaf1 , an enhancer of Usp1 activity , until the Gln - Usp1(Ct ) fragment is selectively destroyed by the Arg / N - end rule pathway . - pubmed
We also show that metabolic stabilization of Gln - Usp1(Ct ) results in a decreased monoubiquitylation of PCNA and in a hypersensitivity of cells to ultraviolet irradiation . - pubmed
Thus , in addition to its other functions in DNA repair and chromosome segregation , the Arg / N - end rule pathway regulates genomic stability through the degradation - mediated control of the autocleaved Usp1 deubiquitylase . - pubmed
By substitution of three conserved amino acids , found in all electrogenic isoforms , at corresponding sites in NaPi - IIc , electrogenicity was re - established and the Na(+)/P i stoichiometry increased from 2:1 to 3:1 . - pubmed
Fluorometric recordings from AAD - IIc mutants with Cys substituted at functionally important sites established that AAD - IIc undergoes substrate- and voltage - dependent conformational changes that correlated qualitatively with its presteady - state kinetics . - pubmed
This study was designed to investigate the potential role of induction of HSP70 using geranylgeranylacetone ( GGA ) on testicular damage caused by CCl4 . - pubmed
Serum testosterone , testicular lactate dehydrogenase ( LDH ) and alkaline phosphatase ( ALP ) , testicular malondialdehyde ( MDA ) , total nitrite and total antioxidant capacity ( T - AOC ) were measured . - pubmed
Group II showed lower values of gonado - somatic index , serum testosterone , testicular LDH , ALP , T - AOC and greater values of testicular MDA and total nitrite than in control . - pubmed
Group III had higher values of gonado - somatic index , serum testosterone , testicular LDH , ALP , T - AOC with less testicular MDA and total nitrite than in group II . - pubmed
These findings suggest that GGA - induced activation of HSP 70 significantly alleviate CCl4 inflicting testicular damage by HSP 70 mediated cytoprotection and antioxidant effects . - pubmed
Long - lasting distortion of GABA signaling in MS / DB neurons after binge - like ethanol exposure during initial synaptogenesis . - pubmed
The impact of ethanol in vivo was long lasting as most changes in MS / DB GABAAR mPSCs were still observed as late as PD 60 - 85 . - pubmed
Maturing MS / DB neurons in naive brain slices PD 4 - 16 showed increasing mPSC frequency , decay kinetics , and zolpidem sensitivity that were nearly identical to our earlier findings in cultured septal neurons ( DuBois et al . - pubmed
Previously , we showed ethanol treatment of cultured embryonic day 20 septal neurons distorts the maturation of GABAAR mPSCs predicting that early stages of GABAergic transmission in MS / DB neurons are vulnerable to intoxication injury ( DuBois et al . - pubmed
From eight gp21 - analyzed sequences one amino acid change ( Y477H ) was associated with the switch of a helix to coil structure at secondary structure prediction . - pubmed
From 10 HBZ analyzed sequences , two amino acid changes were identified ( S9P and T95I ) at the activation domain . - pubmed
It was found that the inclusion of rIL-2 in the R - CHOP regimen increased the effectiveness of the treatment of B - cell NHL with a high - risk of poor disease 's course . - pubmed
coli strain containing PAL1 , 4CL1 , and CUS led to the production of dicinnamoylmethane at a high level of 0.36 g / l. Supplement of 2 - fluoro - phenylalanine yielded two fluorinated dicinnamoylmethane derivatives , 6,6 '- difluoro - dicinnamoylmethane and 6 - fluoro - dicinnamoylmethane , of which the latter is a new curcuminoid . - pubmed
Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate - polyacrylamide gel electrophoresis , and 28,800 Da determined by gel filtration . - pubmed
Access of hydrogen - radicals to the peptide - backbone as a measure for estimating the flexibility of proteins using matrix - assisted laser desorption / ionization mass spectrometry . - pubmed
The MALDI - ISD spectra of bovine serum albumin ( BSA ) , myoglobin and thioredoxin show discontinuous intense ion peaks originating from one - side preferential cleavage at the N - Calpha bond of Xxx - Asp , Xxx - Asn , Xxx - Cys and Gly - Xxx residues . - pubmed
RESULTS : The unstimulated salivary flow rate measured by MST at the end of 3rd minute was 13.7 + - 10.08 , 19.86 + - 8.95 and 31.0 + - 5.4 mm and by spitting method was 0.12 + - 0.07 , 0.19 + - 0.10 and 0.30 + - 0.75 ml / min in TCA , SSRI and controls respectively ( p<0.001 ) . - pubmed
AIMS : A high consumption of fructose leads not only to peripheral changes in insulin sensitivity and vascular function , but also to central changes in several brain regions . - pubmed
Given the role of the endogenous cannabinoid system in the control of energy intake , we undertook a pilot study to determine whether a high fructose diet produced changes in brain CB1 receptor functionality . - pubmed
CB1 receptor functionality was measured autoradiographically as the increase in [ ( 35 ) S ] GTPgammaS binding produced by the agonist CP55,940 . - pubmed
Two - way robust Wilcoxon analyses for each brain region indicated that the dietary treatment did not produce significant main effects upon agonist - stimulated [ ( 35 ) S ] GTPgammaS binding in any of the regions , in contrast to a significant main effect upon both leptin and adiponectin levels in the blood . - pubmed
However , a MANCOVA of the data controlling for leptin and adiponectin as co - variables identified a significant effect of glucose and fructose treatment for five weeks upon the [ ( 35 ) S ] GTPgammaS response in the ventromedial hypothalamus , a region of importance for regulation of appetite . - pubmed
Photoperiod treatment ( either SD or LD ) failed to induce significant changes in the HPG activity , as measured by androgen levels and gonadosomatic index . - pubmed
However , SD increased the intensity of aggressive behaviors and shortened the time to settle a dominance hierarchy in an androgen - independent manner . - pubmed
The higher androgen response to a social challenge in residents under SD may be explained by the time course of the androgen response that due to the long time it takes to fight resolution under LD , might have been delayed . - pubmed
A novel method combining Inductively Coupled Mass Spectrometry ( ICP - MS ) with solid - phase extraction ( SPE ) to separate and purify radium isotopes from the matrix elements in high salinity solutions is developed in this study . - pubmed
Radium separation is accomplished using a combination of a strong - acid cation exchange resin to separate barium and radium from other ions in the solution and a strontium - specific resin to isolate radium from barium and obtain a sample suitable for analysis by ICP - MS . - pubmed
Method optimization achieved high radium recovery ( 101 + - 6 % for standard mode and 97 + - 7 % for collision mode ) for synthetic Marcellus Shale wastewater ( MSW ) samples with total dissolved solids as high as 171,000 mg / L. Ra-226 concentration in actual MSW samples with TDS as high as 415,000 mg / L measured using ICP - MS matched very well with the results from gamma spectrometry . - pubmed
Due to the inherent heterogeneity of conjugation to the multiple lysines or cysteines found in mAbs , significant research efforts are now being directed toward the production of discrete , homogeneous ADC products , via site - specific conjugation . - pubmed
These site - specific conjugations may involve genetic engineering of the mAb to introduce discrete , available cysteines or non - natural amino acids with an orthogonally - reactive functional group handle such as an aldehyde , ketone , azido , or alkynyl tag . - pubmed
As a consequence of profound acid suppression , serum gastrin during vonoprazan therapy rose to much higher levels than those during lansoprazole therapy , pointing to a requirement for the assurance of long - term safety of vonoprazan . - pubmed
We report a case of multiple lung metastases of an epithelioid sarcoma originating in the inguinal area that we treated with the multikinase inhibitor pazopanib . - pubmed
The results revealed that siomycin A induced apoptosis by influencing the downstream events of FoxM1 , including inhibiting the expression of Bcl-2 and Mcl-1 , as well as leading to caspase-3 cleavage . - pubmed
Successful treatment of FKBP51 - expressed multicentric reticulohistiocytosis using combination therapy with low - dose denosumab and tacrolimus . - pubmed
We identified dozens of proteins that responded to zinc in a ColR - independent manner , among others , known metal efflux systems CzcCBA1 , CzcCBA2 , CadA2 and CzcD. - pubmed
Despite the relatively modest ColR - dependent changes of wild - type , colR deficiency resulted in drastic proteome alterations , with 122 proteins up- and 62 down - regulated by zinc . - pubmed
This response likely alleviates the zinc stress , as the AlgU - regulated alginate regulator AmrZ was shown to contribute to zinc tolerance of colR knockout . - pubmed
Thus , the ColRS system is important for zinc homeostasis , and in its absence , alternative stress response pathways are activated to support the zinc tolerance . - pubmed
BX-795 is an inhibitor of 3 - phosphoinositide - dependent kinase 1 ( PDK1 ) , but also a potent inhibitor of the IKK - related kinase , TANKbinding kinase 1 ( TBK1 ) and IKKe . - pubmed
BX-795 treatment dose - dependently suppressed the expression of two HSV immediate - early ( IE ) genes ( ICP0 and ICP27 ) and the late gene ( gD ) at 12 h postinfection . - pubmed
HSV-2 infection resulted in the activation of PI3 K and Akt in the host cells , and BX-795 treatment inhibited HSV-2 - induced Akt phosphorylation and activation . - pubmed
HSV-2 infection increased the phosphorylation of JNK and p38 , and reduced ERK phosphorylation at 8 h postinfection in the host cells ; BX-795 treatment inhibited HSV-2 - induced activation of JNK and p38 MAP kinase as well as the phosphorylation of c - Jun and ATF-2 , the downstream targets of JNK and p38 MAP kinase . - pubmed
Furthermore , SB203580 ( a p38 inhibitor ) or SP600125 ( a JNK inhibitor ) dose - dependently inhibited the viral replication in the host cells , whereas PD98059 ( an ERK inhibitor ) was not effective . - pubmed
Moreover , BX-795 blocked PMA - stimulated c - Jun activation as well as HSV-2 - mediated c - Jun nuclear translocation . - pubmed
BX-795 dose - dependently inhibited HSV-2 , PMA , TNF - alpha - stimulated AP-1 activation , but not HSV - induced NF - kappaB activation . - pubmed
Overexpression of p38 / JNK attenuated the inhibitory effect of BX-795 on HSV replication . - pubmed
BX-795 completely blocked HSV-2 - induced MKK4 phosphorylation , suggesting that BX-795 acting upstream of JNK and p38 MAP kinase . - pubmed
In conclusion , this study identifies the anti - HSV activity of BX-795 and its targeting of the JNK / p38 MAP kinase pathways in host cells . - pubmed
The deduced PoVtn protein is comprised of 438 amino acids with a 19 - amino - acid signal peptide sequence ( 1Met-19Ala ) at the N - terminus . - pubmed
The unique properties of the SPIONs - core and the functional gold ( Au)-shell together ( SPIONS / Au core / shell or CS ) have a wide range of biomedical applications including , but not limited to , magnetic resonance imaging ( MRI ) , dual modality MRI / computed tomography ( CT ) , photo - induced and magnetic fluid hyperthermia ( MFH ) , drug delivery , biosensors , and bio - separation . - pubmed
Herein , we report synthesis and evaluation of new twenty six small molecules against beta amyloid ( Abeta)-induced opening of mitochondrial permeability transition pore ( mPTP ) using JC-1 assay which measures the change of mitochondrial membrane potential ( DeltaPsim ) . - pubmed
The neuroprotective effect of seventeen compounds against Abeta - induced mPTP opening was superior to that of the standard Cyclosporin A ( CsA ) . - pubmed
Fifteen derivatives eliciting increased green to red fluorescence percentage less than 40.0 % were evaluated for their impact on ATP production , cell viability and neuroprotection against Abeta - induced neuronal cell death . - pubmed
A method for rapid screening of fipronil and its metabolites in egg and egg products was developed by liquid chromatography coupled with quadrupole time - of - flight mass spectrometry ( LC - QTOF MS ) . - pubmed
The separation was performed on a Poroshell 120 EC C18 column ( 150 mmx3 mm , 2.7 mum ) with gradient elution using water and acetonitrile as mobile phases . - pubmed
Blood samples were collected from eight birds per treatment at week 4 ( starter phase ) and week 8 ( finisher phase ) of the experiment for the determination of plasma 3,5,3 '- triiodothyronine ( T3 ) , superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) . - pubmed
Gadolinium - enhanced magnetic resonance imaging T1 - weighted sequences have been demonstrated to be adequate for this purpose . - pubmed
Note that we used the Medtronic StealthStation S8 ( Minneapolis , Minnesota , USA ) and gadolinium - enhanced magnetic resonance imaging T1 - weighted sequences to plan 4 different surgical procedures ( needle biopsy , parasagittal meningioma , double metastases , and high - grade glioma ) . - pubmed
Proteobacteria were more abundant in the AT group than in the MST group and may include denitrifying bacteria contributing to nitrous oxide ( N2O ) emission or aerobic bacteria stimulated by oxygen . - pubmed
We also investigated the clinical relevance of an OqxR loss - of - function mutation , particularly in the context of beta - lactam susceptibility . - pubmed
RamR loss activated AcrAB efflux pump production ~5 - fold and this reduced beta - lactam susceptibility , conferring ertapenem non - susceptibility even in the absence of a carbapenemase . - pubmed
This reduced fluoroquinolone susceptibility but had little impact on beta - lactam susceptibility even in the presence of a beta - lactamase . - pubmed
This means that only RamR mutants have significantly reduced beta - lactamase - mediated beta - lactam susceptibility and therefore represent a greater clinical threat . - pubmed
Results : Twenty - four weeks of exposure to smokeless tobacco products or nicotine significantly decreased the levels of circulating Flk+/Sca+ endothelial progenitor cells . - pubmed
Neither smokeless products nor nicotine affected plasma lipoproteins , platelet activation , or systemic insulin sensitivity . - pubmed
Increased formation of proinflammatory cytokines such as TNFalpha by nicotine or Copenhagen snuff may lead to vascular inflammation and thereby exacerbate atherogenesis . - pubmed
We hypothesized that trauma experience would have a negative impact on brain function , resulting in altered basal cortisol levels via dysregulated neural control over the HPA axis system . - pubmed
Molybdenum carbides and nitrides preferentially activate C O and C - OH bonds over C C and C - C bonds in liquid - phase hydrogenation of bioderived furfural , leading to highly selective formations of furfuryl alcohol ( FA ) and its subsequent hydrogenolysis product ( 2 - methyl furan ( 2 - MF ) ) . - pubmed
, & gt ; 90 % yields of FA in methanol solvent and of 2 - MF in 2 - butanol solvent at 423 K ) . - pubmed
Investigating the Effect of Demographics , Clinical Characteristics , and Polymorphism of MDR-1 , CYP1A2 , CYP3A4 , and CYP3A5 on Clopidogrel Resistance . - pubmed
However , known polymorphisms of MDR-1 , CYP1A2 , CYP3A4 , and CYP3A5 were not associated with treatment resistance when compared to responders to clopidogrel therapy . - pubmed
My early studies focused on understanding how insulin promotes glucose transport into adipocytes , a classic model of highly insulin - responsive target cells . - pubmed
We found that the AMP - activated protein kinase ( AMPK ) pathway mediates leptin 's effects on fatty acid oxidation in muscle and also plays a role in leptin 's anorexigenic effects in the hypothalamus . - pubmed
Cell viability was determined using XTT assay and was assessed at 0 , 24 and 48 h intervals after a 10 - min - long treatment , followed by thorough washes , or at 24 and 48 h of treatment at TXA concentrations of 20 , 50 , 70 and 100 mg / ml . - pubmed
Valproic acid ( VPA ) is a histone deacetylase ( HDAC ) inhibitor that has shown positive results on SMA both in experimental and cohort studies . - pubmed
The primary outcome was the efficacy of VPA in terms of gross motor function and expression of both full - length spinal motor neuron ( SMN ) gene ( FL - SMN ) and exon 7 - lacking SMN . - pubmed
The overall effect estimate , comparing pre- and post - VPA treatment , regardless of carnitine co - administration and design of the studies , showed significant improvement in gross motor function ( standard mean difference [ SMD ] = 0.302 , 95 % confidence interval [ CI ] 0.048 - 0.556 , P = 0.02 ) using the Hammersmith Functional Motor Scale ( HFMS ) , Modified Hammersmith Functional Motor Scale ( MHFMS ) , and MHFMS - Extend , with no significant heterogeneity . - pubmed
No significant differences were found between pre- and post - VPA treatment co - administered with carnitine , in terms of the change in FL - SMN and exon 7 - lacking SMN . - pubmed
In conclusion , farnesol has antiarrhythmic effect mediated by reducing of ICa , L and IK along with a decrease of reactive oxygen species production and normalized Ca2 + sparks and waves . - pubmed
Azoramide is identified as a new compound with the dual properties for the improvement of ER - folding capacity in various cells as well as for the treatment of T2DM . - pubmed
Therefore , here , we aimed to investigate the direct effect of azoramide on insulin signaling in insulin - resistant ( IR ) cardiomyocytes using IR - modelled ventricular cardiomyocytes . - pubmed
This work shows that , when the electron - donating ability of the N - substitution is further increased in the presence of a carbanion or phenoxide , spontaneous electron transfer ( ET ) occurs and results in radical anions , verified with electron - paramagnetic resonance ( EPR ) spectroscopy . - pubmed
We analyzed the photoantimicrobial effect caused by red light activation of methylene blue ( MB - APDT ) and by blue light ( BL ) activation of endogenous microbial pigments against 5 clinically relevant pathogens . - pubmed
Durable Response of Low - Dose Afatinib plus Cetuximab in an Adenocarcinoma Patient with a Novel EGFR Exon 20 Insertion Mutation . - pubmed
Orientin suppresses oxidized low - density lipoproteins induced inflammation and oxidative stress of macrophages in atherosclerosis . - pubmed
The scavenger receptor CD 36 of ox - LDL was significantly downregulated after the treatment of orientin . - pubmed
Inhibition of ROS generation and increasing of eNOS expression by Orientin treatment was used to show the alteration of oxidative stress . - pubmed
Moreover , the expression levels of Angiopoietin - like 2 ( angptl2 ) and NF - kappaB were significantly upregulated after cells induced by ox - LDL , whereas orientin significantly reversed the effects of ox - LDL . - pubmed
Orientin inhibited ox - LDL - induced inflammation and oxidative stress , and CD36 may be the key regulator during Orientin action . - pubmed
The mRNA levels of genes involved in carbon fixation , namely , rbcL and rbcS , were clearly increased compared with those under shelter - free conditions , resulting in improved CO2 utilization . - pubmed
Furthermore , the transcription levels of genes involved in chlorophyll ( hemA , hemN , and chlH ) and carotenoid synthesis ( crtB , PDS , crtISO , and lcyB ) in the sheltered leaves peaked earlier than those in the unsheltered leaves , thereby promoting organic matter accumulation in leaves . - pubmed
Remarkably , the expression levels of key genes involved in the metabolic pathways of phenylpropanoid ( PAL , C4H , and 4CL ) and flavonoid ( CHS , CHI , F3'H , DFR , and ANS ) in the sheltered fruits were also up - regulated earlier than of those in the unsheltered fruits , conducive to an increase in anthocyanin content in the fruits . - pubmed
Particularly , ibutamoren , known as an agonist of growth hormone secretagogue receptor ( GHSR ) , showed the most promising antiviral activity with a 50 % effective concentration of 0.2 muM , a 50 % cytotoxic concentration of 42.4 muM , and a selectivity index of 222.8 . - pubmed
Although sFlt-1 is a soluble protein , it contains a binding site for the extracellular matrix component heparan sulfate . - pubmed
Consequently , the placenta becomes a potential storage site for large amounts of sFlt-1 bound to extracellular heparan sulfate . - pubmed
Additionally , it should be noted that sFlt-1 can bind to the anticoagulant unfractionated heparin due to its molecular mimicry to heparan sulfate . - pubmed
However , it remains unknown whether unfractionated heparin can compete with heparan sulfate for binding of localized sFlt-1 . - pubmed
Real - time quantitative PCR of the explants showed no change in sFlt-1 or heparanase-1 mRNA expression , eliminating increased production and enzymatic cleavage of heparan sulfate as causes for sFlt-1 media increase . - pubmed
These data demonstrate that chronic unfractionated heparin treatment is able to displace matrix - bound sFlt-1 into the maternal circulation to such a degree that mean arterial pressure is significantly affected . - pubmed
MEDI0562 , an agonistic humanized monoclonal antibody ( mAb ) , specifically binds to the costimulatory molecule OX40 . - pubmed
MEDI0562 induced increased Ki67 + CD4 + and CD8 + memory T - cell proliferation in the periphery and decreased intratumoral OX40 + FOXP3 + cells . - pubmed
During a follow - up visit , she showed signs of acute exacerbation and she was switched to benralizumab during her acute phase with a rapid , dramatic amelioration of respiratory symptoms and pulmonary function , without concomitant systemic corticosteroid administration . - pubmed
In potentiostatic electrolysis of 50 mM NaCl solutions , dramatic improvement ( 0.61 mmol cm-2 hr-1 at 2.5 V NHE ) was noted by simple coating of thin ( ~2 mum ) TiO2 layer from ball - milled TiO2 nanoparticle ( 80 - 100 nm ) suspension , even with moderate elevation in voltammetric wave . - pubmed
However , Bi - doped TiO2 layers prepared from polyol - mediated or co - precipitation methods marked the highest CERCS ( ~100 % ) and EERCS ( 8.16 mmol Wh-1 at 2.5 V NHE ) by increased mixing level and effective shift in surface charge . - pubmed
In the present study , using 10 muM of gibberellic acid ( GA3 ) , or gibberellin biosynthesis ( GA ) inhibitors - ancymidol ( A ) and paclobutrazol ( P)-the dynamic changes in soluble sugars , fructose and glucose content , fresh weight and sprouting capacity were investigated . - pubmed
meleagris bulbs were cultured on medium with GA3 and GA inhibitors for 1 , 2 and 5 weeks at two different temperatures ( 24 and 7 C ) . - pubmed
The sensitivity of bulbs to GA inhibitors differed and significantly affected sugar content in bulbs . - pubmed
BCR - ABL was assessed by quantitative rt - PCR at days 0 and 90 of imatinib therapy . - pubmed
The critical binding region of ACE2 is an ~ 30 - amino - acid ( aa)-long helix . - pubmed
NYBSP-2 and NYBSP-4 also prevented the formation of the virus - induced CPE with an IC100 of about 33 muM. - pubmed
The aims of this study were ( i ) to determine whether different metabolic profiles of B - vitamins and vitamin A in Brazilian children and adolescents were positively or negatively related to homocysteine and omega-3 fatty acids using k - means clustering analysis , ( ii ) compare nutrient intakes and metabolites between the different metabolic profiles , ( iii ) evaluate if the statistically significant metabolites found between the metabolic groups , can predict the variation of leukotriene A4 hydrolase ( LTA4H ) levels , a biomarker of low - grade inflammation , in the total studied population . - pubmed
Multiple linear regression analyses showed that blood cobalamin , riboflavin , pyridoxal and homocysteine combined , explained 9.0 % of LTA4H levels variation in the total studied population . - pubmed
The combined levels of the riboflavin , pyridoxal , cobalamin and homocysteine biomarkers can predict the variation of LTA4H in the total population studied , but it is not clear how this regulation occurs . - pubmed
Vibrational spectra of H14 - Cp * Rh - Cl and D14 - Cp * Rh - Cl were captured via inelastic neutron spectroscopy ( INS ) and assigned using density functional theory ( DFT ) phonon calculations . - pubmed
The therapeutic potential of TAK-242 , a selective TLR4 inhibitor , was evaluated for OD . - pubmed
TAK-242 treatment significantly reduced the level of IL-1beta , IL-6 , and TNF - alpha mRNA expression , as well as activation of microglia and astrocytes in the OB tissues . - pubmed
U0126 is a specific inhibitor of the extracellular signal - regulated protein kinases 1 and 2 ( ERK1 / 2 ) signaling pathway and has neuroprotective effects . - pubmed
Prospective and cross - sectional studies that evaluated the association between Hg exposure and the levels of thyroid stimulating hormone ( TSH ) , triiodothyronine ( T3 ) , thyroxine ( T4 ) , free triiodothyronine ( FT3 ) , and free thyroxine ( FT4 ) were included . - pubmed
The presence of Hg in blood may significantly increase the levels of TSH ( beta=0.55 ; 95%CI : 0.20,0.90 ; p < 0.001 ) and FT4 ( beta=0.47 ; 95%CI : 0.11,0.82 ; p < 0.001 ) , with the opposite association in T4 ( beta=-0.02 ; 95%CI : 0.02 , -0.01 ; p < 0.001 ) . - pubmed
For the subgroup analysis , blood Hg was positively correlated with TSH levels in children and adolescents ( beta=0.62 ; 95%CI : 0.09 , 1.15 ; p < 0.001 ) and FT4 levels in pregnant women ( beta=1.00 ; 95%CI : 0.99 , 1.00 ; p < 0.001 ) respectively . - pubmed
CONCLUSIONS : This meta - analysis indicates that exposure to Hg in blood could significantly corrrelate with the levels of TSH , T4 , and FT4 in the general population . - pubmed
Inactivation of tobacco ribulosebisphosphate carboxylase by 2,3 - butanedione . - pubmed
A fluorometric method for the determination of naproxen in serum , albumin solutions and protein free buffer solutions is described . - pubmed
The in vitro binding of naproxen in albumin solutions and serum was studied by equilibrium dialysis . - pubmed
At proestrus , the uptake of 125I - HCG was unaffected by the LH surge at 18.00 h but had decreased slightly at 24.00 h. The implications of these data in relation to the regulation of receptor sites , is discussed . - pubmed
We recorded a bioassay for prolactin using densitometric analysis on polyacrylamide gels of caseins produced by mammary explants in vitro . - pubmed
Both plasma and cerebrospinal fluid levels of oxytocin were measured under similar conditions , following CRF and morphine treatments . - pubmed
Morphine and CRF potentiated the effects of vaginocervical stimulation on these behaviours . - pubmed
The results show that maternal experience is essential for oxytocin to promote proceptive behaviours and that morphine and CRF potentiate the effects of vaginocervical stimulation on acceptance behaviour . - pubmed
This wound was closed using either a sutureless tissue weld created by a microscope - mounted CO2 laser ( 153 W / cm2 ) ( Group I , N = 61 ) or with a running 6 - O polypropylene suture ( Group II , N = 58 ) . - pubmed
Fluorography after photoaffinity labeling as well as immunoblotting with a specific polyclonal antibody revealed that the human androgen receptor migrated as a closely spaced 110 kD doublet on SDS - polyacrylamide gels . - pubmed
Previous studies demonstrating hydrolysis of phosphatidylinositol bisphosphate ( PIP2 ) and generation of inositol phosphates in neutrophils exposed to 20.0 mM NaF provide indirect evidence that activation of phospholipase - associated guanine nucleotide regulatory protein , a guanine nucleotide binding protein which regulates the activation of a membrane inositol - specific phospholipase C , is an early event in the neutrophil stimulus - response pathway triggered by fluoride . - pubmed
To further explore the involvement of guanine nucleotides in functional responses of intact neutrophils triggered by fluoride , we preincubated cells with 2 - beta - D - ribofuranosylthiazole-4 - carboxamide ( tiazofurin ) , a selective inhibitor of inosine monophosphate dehydrogenase , to diminish guanine nucleotide synthesis and then compared superoxide generation induced by FMLP , PMA , digitonin , and 20.0 mM NaF to intracellular levels of guanine nucleotides . - pubmed
The effects of tiazofurin on guanine nucleotides levels were not observed when neutrophils were preincubated at 4 degrees C. AT 37 degrees C , tiazofurin also decreased intracellular ATP and ADP levels but adenine nucleotide depletion was less pronounced than guanine nucleotide depletion for each concentration of tiazofurin used . - pubmed
Acute exposure by inhalation to 100 mg / m3 silica dust produced persistent pulmonary inflammation , as well as long - term changes in pulmonary alveolar macrophages ( AM ) typical of macrophage activation . - pubmed
After silica inhalation , the following changes were observed constantly in bronchopulmonary lavage cell samples : increased numbers of polymorphonuclear leukocytes ( PMN ) , hypersegmented PMN , binucleated AM , increased AM phagocytic indices and capacities , and increased mycocidal activity . - pubmed
Exposure to 500 mg / m3 graphite produced transient inflammation and AM activation for about 24 - 48 hr . - pubmed
The results of this study provide strong evidence at the level of mRNA expression that ProEnk A mRNA is expressed preferentially in the adrenaline synthesizing cells within the adrenal medulla . - pubmed
This reduction was 2.06 + /- 1.8 for the control , 1.96 + /- 1.27 when captopril ( CAP ) ( 10(-5 ) M ) was added to the perfusion liquid and 1 + /- 0.42 when perindopril ( PER ) ( 1.2 x 10(-5 ) M ) was added to the perfusion liquid ( p less than 0.05 vs CAP ) . - pubmed
In the presence of CAP or PER the effects of AII were the same , however in the presence of SAR these effects were prevented . - pubmed
This seizure suppressant action was dose dependent , spatially specific for the area of the SN and was not blocked by naloxone ( 2 mg / kg i.p . ) - pubmed
Chemical modification of aphidicolin and the inhibitory effects of its derivatives on DNA polymerase alpha in vitro . - pubmed
In the presence of 0.1 % Triton X-100 extensive degradation of the receptor by trypsin is observed . - pubmed
Assays of extracts from the lapel filters for Cat-1 content by radioimmunoassay and for allergenic activity by leukocyte histamine release demonstrated a significant correlation ( p less than 0.05 ) between the results of the two assays . - pubmed
The determination of the dissociation velocity constants of rabbit uterine cytosol receptor - oestrogen complexes . - pubmed
The first kinase , present in greatest activity in microsomal extracts , appears to be identical to casein kinase I by characteristic molecular size on gel filtration ( Mr 40,000 ) and sodium dodecyl sulfate - gel electrophoresis ( Mr 34,000 ) , autophosphorylation of this single subunit , inability to efficiently utilize GTP , and resistance to inhibition by heparin and 2,3 - diphosphoglycerate . - pubmed
The second kinase , predominant in cytosol , appears to be identical to casein kinase II by characteristic molecular size on gel filtration ( Mr 150,000 ) , an autophosphorylated subunit of Mr 25,000 , a Km for GTP nearly equal to that of ATP , inhibition by heparin and 2,3 DPG , and relative substrate specificity . - pubmed
Despite the incorporation of up to 2 mol 32P / mol carboxylase subunit ( kinase I ) and 0.6 mol / subunit ( kinase II ) , phosphorylation by either kinase causes no change in carboxylase activity . - pubmed
The site(s ) phosphorylated by each kinase and by the cAMP - dependent protein kinase on carboxylase appear to be clustered on a Mr 16,000 cyanogen bromide peptide that is readily released on incubation with trypsin . - pubmed
Preparation of a biocompatible albumin - coated ion exchange resin for bilirubin removal from the blood of jaundiced newborns . - pubmed
The choice of the appropriate ionic form of the resin and the proper albumin coating and crosslinking procedure assures a high bilirubin adsorption capacity and excellent blood compatibility of the resin . - pubmed
The albumin coated resin removes in vitro 80 - 90 % of the bilirubin initially present in the plasma . - pubmed
After treatment with clomiphene and HCG in 16 women one or more oocytes were harvested . - pubmed
The results of this study are as follows : FFA was significantly lower , while pyruvate was significantly higher at rest after a high CHO diet than after a low CHO diet ( P less than 0.05 ) . - pubmed
The cells were cryopreserved and stored under liquid nitrogen for intervals of 2 , 4 , 6 , 8 , or 12 wk , then assayed for viability ( using the trypan dye exclusion method as well as the NADH - dependent cytochrome c reductase ( cytochrome c ) assay ) and cellular function ( as documented by the measurement of in vitro aryl hydrocarbon hydroxylase ( AHH ) induction by benzanthracene ( BA ) and by quantitation of PAM particulate phagocytosis ) . - pubmed
Similarly , when AHH induction was fluorometrically quantitated in PAM cultured for 24 h , there was no decrease in BA - induced AHH levels after a 2 - wk cryopreservation period . - pubmed
However , AHH levels decreased slightly compared with those in the 0 - time control cells ( p less than 0.004 ) , when PAM were stored for longer intervals of 4 , 6 , or 8 wk . - pubmed
The purified enzyme was free of other known brush border enzymes and appeared homogeneous in immunoelectrophoresis and polyacrylamide gel electrophoresis in the presence of SDS . - pubmed
Increased thyrotrophin and prolactin secretion induced by domperidone in hypothyroid subjects . - pubmed
Domperidone ( 4 mg iv ) induced a clear - cut and sustained rise in both plasma TSH and Prl , with peak levels occurring 15 and 30 min post - injection , respectively . - pubmed
The increments in plasma Prl after domperidone were significantly higher than those in plasma TSH . - pubmed
Clomipramine - induced agranulocytosis and its treatment with G - CSF . - pubmed
When prolactin and growth hormone were coinjected , growth hormone also proved unable to oppose the Na+ retaining effect of prolactin , in both brackish and fresh water . - pubmed
Here , to test whether beta 2 carboxyl sequences can rescue the functional failure of the beta 3 isoform in spermatogenesis , we constructed a gene encoding a chimeric protein , beta 3 beta 2C , in which beta 3 sequences in the carboxyl region are replaced with those of beta 2 . - pubmed
Truncated forms of gC and gD , gC1(457 t ) , gC2(426 t ) , and gD1(306 t ) , lacking the transmembrane and carboxyl regions were expressed in the baculovirus system . - pubmed
Losartan , nonpeptide angiotensin II - type 1 ( AT1 ) receptor antagonist , attenuates pressor and sympathoexcitatory responses evoked by angiotensin II and L - glutamate in rostral ventrolateral medulla . - pubmed
We investigated the effect of losartan , a nonpeptide angiotensin II ( Ang II )- type 1 ( AT1 ) receptor antagonist , on the responses evoked by Ang II and L - glutamate ( L - Glu ) in the rostral ventrolateral medulla ( RVLM ) . - pubmed
Responses of mean arterial pressure ( MAP ) , heart rate ( HR ) and splanchnic sympathetic nerve activity ( SNA ) to microinjection of Ang II ( 100 pmol ) or L - Glu ( 2 nmol ) into the RVLM were examined following microinjection of losartan ( 10 pmol-10 nmol ) . - pubmed
Ang II increased MAP ( 16 + /- 1 mmHg in SHR and 16 + /- 1 mmHg in WKY ) and SNA ( 9 + /- 1 % and 10 + /- 1 % , respectively ) , which were significantly ( P & lt ; 0.01 ) attenuated by pretreatment with losartan ( 100 pmol-10 nmol ) in both strains . - pubmed
Reconstitution of the activity of dialyzed arginase by manganese is a two - phase process ; the second phase is independent of the cation concentration , with a half - time recovery ( t1/2 ) of 10.77 min . - pubmed
The apparent Km for Mn2 + is 280 microM and 10.5 microM for enzyme dialyzed for 24 and 72 hr , respectively . - pubmed
Treatment with 5 mM EDTA at pH 6 totally inhibits enzyme activity , which is reconstituted by Mn2 + . - pubmed
Results obtained with iodoacetamide treatment suggest the existence of sulphydryl groups accessible only when the enzyme is dialyzed . - pubmed
Significant increases in superoxide dismutase ( SOD ) , glutathione peroxidase and catalase ( CAT ) activities were observed at 15 or 18 hours after the injection of paraquat . - pubmed
In the lung , lipid peroxide concentration decreased significantly and the activities of SOD and CAT increased at 15 hours after injection of paraquat . - pubmed
However , the enzyme induction was produced by paraquat without the increase of TBARS in the lung . - pubmed
During endogenous feeding , despite larval drinking , MT mRNA levels did not appear to be inducible by exposure to Cd in the water within 3 days of hatching but were induced three- to fivefold after 48 hr exposure to waterborne Cd at all later stages of development . - pubmed
The results suggest that Cd tolerance is associated with metal induction of MT gene transcription and that the enhanced sensitivity of very early stage larvae is attributable to a deficiency in MT gene transcription . - pubmed
Blockade of DNA synthesis induced by platelet - derived growth factor by tranilast , an inhibitor of calcium entry , in vascular smooth muscle cells . - pubmed
At lower concentrations , PDGF induced oscillatory changes in [ Ca2 +]c , and reduction of extracellular calcium attenuated the oscillation . - pubmed
An antiallergic compound , tranilast , abolished the sustained phase of [ Ca2 +]c induced by 1 nM PDGF . - pubmed
Tranilast also inhibited the oscillatory changes in [ Ca2 +]c induced by 200 pM PDGF . - pubmed
In addition , PDGF - induced calcium influx in the late G1 phase , as assessed by measuring the initial uptake of 45Ca , was inhibited by tranilast in a concentration - dependent manner . - pubmed
Tranilast also inhibited PDGF - augmented DNA synthesis ; the ID50 for the inhibition of DNA synthesis was nearly identical to that for calcium influx . - pubmed
Although tranilast blocked PDGF - induced calcium entry , it did not affect PDGF - mediated autophosphorylation of the PDGF receptor , activation of phosphatidylinositol 3 - kinase , activation of Ras or mitogen - activated protein kinase . - pubmed
Similarly , PDGF - induced elevation of diacylglycerol was not affected by tranilast . - pubmed
These results suggest that the antiallergic drug tranilast inhibits PDGF - induced DNA synthesis by blocking PDGF - mediated calcium entry . - pubmed
Tranilast may be of use in controlling PDGF - induced DNA synthesis in VSMC . - pubmed
Surface damage of the tibial plateau components of knee prostheses made from medical grade ultra - high - molecular - weight polyethylene ( UHMW - PE ) has been attributed to delamination wear caused by a fatigue fracture mechanism . - pubmed
Gelsolin is a calcium - activated actin - binding protein with six subdomains . - pubmed
A Ca2 + -sensitive truncate containing G2 - 6 inhibited the F - actin - depolymerizing activities of both gelsolin and cofilin , while a G2 - 3 truncate was less effective . - pubmed
Cosedimentation assays in the presence of calcium demonstrate that the G2 - 6 truncate binds to F - actin more strongly than the G2 - 3 truncate consistent with a protection mechanism by conformational change of F - actin and/or competitive binding to actin filaments which depends upon the presence of actin filament binding domains . - pubmed
Acting through cell surface receptors , ADP activates platelets resulting in shape change , aggregation , thromboxane A2 production , and release of granule contents . - pubmed
ADP also causes a number of intracellular events including inhibition of adenylyl cyclase , mobilization of calcium from intracellular stores , and rapid calcium influx in platelets . - pubmed
The receptor responsible for the actions of ADP on platelets has been designated the P2 T receptor . - pubmed
In this study we have used ARL 66096 , a potent antagonist of ADP - induced platelet aggregation , and a P2X ionotropic receptor agonist , alpha , beta - methylene adenosine 5 '- triphosphate , to distinguish the ADP - induced intracellular events . - pubmed
ARL 66096 blocked ADP - induced inhibition of adenylyl cyclase , but did not affect ADP - mediated intracellular calcium increases or shape change . - pubmed
These results clearly demonstrate the presence of two distinct platelet ADP receptors in addition to the P2X receptor : one coupled to adenylyl cyclase and the other coupled to mobilization of calcium from intracellular stores through inositol trisphosphates . - pubmed
The synthetic 20 base oligoribonucleotide , which consists of the template sequence CUAACCCUAAC and the adjacent region of the RNA component of human telomerase , was also found to form the complex with the recombinant nm23 - H2 protein . - pubmed
However , at & gt ; 25 micrograms / cm2 MnSOD mRNA levels in silica- and crocidolite- but not in rockwool - exposed cells decreased . - pubmed
These results suggest that : ( 1 ) like crocidolite and silica , rockwool accelerates MnSOD gene expression in bronchoepithelial cells ; ( 2 ) an increase of MnSOD mRNA levels is not accompanied by MnSOD activity elevation ; ( 3 ) in contrast to rockwool , high concentrations ( & gt ; or = 25 micrograms / cm2 ) of crocidolite and silica reduced MnSOD activity and MnSOD mRNA levels . - pubmed
Because oxidants ( H2O2 ) and crocidolite fibers were shown to reduce SOD activity , lack of active MnSOD protein may be caused by inactivation on a post - translational level . - pubmed
The fibronectin synthesis increased 2.3- and 3.9 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of saponin , respectively , compared with that in non - treated cells . - pubmed
Fibronectin synthesis stimulated with 10 microg ml(-1 ) of saponin was inhibited with 69 % by 5 microg ml(-1 ) of an anti - TGF - beta1 antibody . - pubmed
mRNA of TGF - beta type I receptor increased 4.8- and 4.4 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of saponin , respectively , and that of TGF - beta type II receptor also increased 3.4- and 3.2 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of saponin , respectively . - pubmed
The significant increases of TGF - beta type I and II receptors and of fibronectin synthesis were observed at the same concentrations of saponin . - pubmed
TGF - beta content increased 1.74- and 1.87 - fold at conditioned medium of fibroblasts treated with 100 and 250 microg ml(-1 ) of saponin , respectively , higher concentrations than those which accelerated fibronectin synthesis . - pubmed
Furthermore , the active TGF - beta content was below 10 % of total TGF - beta at each concentration of saponin . - pubmed
These results indicate that saponin stimulates fibronectin synthesis through the changes of TGF - beta receptor expressions in fibroblasts . - pubmed
The enzyme exhibited the glucan transfer and Glc production activities that define D - enzymes . - pubmed
D - enzyme also transferred glucans among the outer chains of amylopectin ( using the polysaccharide chains as both donor and acceptor ) and from malto - oligosaccharides into the outer chains of either amylopectin or glycogen . - pubmed
Absence of all three activities co - segregated with the sta11 - 1 mutation , which is known to cause abnormal accumulation of oligosaccharides at the expense of starch . - pubmed
We surmised that the new Cys was most probably cysteinylated as this albumin species would have a mass decrease of 742 Da and be very close to the measured value of 755 Da . - pubmed
Cysteinylation was confirmed when a mass decrease of 863 Da was measured between the proteins after reduction of their disulfide bonds . - pubmed
Previous studies have demonstrated a positive or negative P - wave configuration in lead aVL originating from this area and a change from a biphasic P - wave in V1 during sinus rhythm to a positive P - wave configuration during tachycardia . - pubmed
Experimental results from an isothermal laminar flow reactor at atmospheric pressure are presented on the chemical composition in the post - oxidative region of two sooting fuel - rich pre - mixed mixtures diluted in nitrogen . - pubmed
Signal transduction inhibitor ( STI571 ) ( formerly CGP57 148B ) , is an ABL specific , tyrosine kinase inhibitor . - pubmed
Divalent metal transporter 1 ( DMT1 ) is the major transferrin - independent iron uptake system at the apical pole of intestinal cells , but it may also transport iron across the membrane of acidified endosomes in peripheral tissues . - pubmed
Iron transport and expression of the 2 isoforms of DMT1 was studied in erythroid cells that consume large quantities of iron for biosynthesis of hemoglobin . - pubmed
Interestingly , iron release from transferrin inside the endosome is normal in mk / mk reticulocytes , suggesting a subsequent defect in Fe (++ ) transport across the endosomal membrane . - pubmed
Experiments with isoform - specific anti - DMT1 antiserum strongly suggest that it is the non - iron - response element containing isoform II of DMT1 that is predominantly expressed by the erythroid cells . - pubmed
Together , these results provide further evidence that DMT1 plays a central role in iron acquisition via the transferrin cycle in erythroid cells . - pubmed
Efficient one - pot synthesis of the 2 - aminocarbonylpyrrolidin-4 - ylthio - containing side chain of the new broad - spectrum carbapenem antibiotic ertapenem . - pubmed
Starting material N -( O , O - diisopropyl phosphoryl )- trans-4 - hydroxy - L - proline is converted in a one - pot process to ( 2S )- cis-3 -[[( 4 - mercapto-2 - pyrrolidinyl ) carbonyl ] amino ] benzoic acid monohydrochloride in 70 - 75 % overall yield via a series of six reactions . - pubmed
Photoconsumption of oxygen in photosystem II preparations under impairment of the water - oxidizing complex . - pubmed
Oxygen consumption in photosystem II ( PSII ) preparations in the light was 2 micromol O2 / h per mg Chl at weakly acidic and at neutral pH values . - pubmed
The addition of either artificial electron donors for PSII such as MnCl2 or diphenylcarbazide , or diuron as an inhibitor of electron transfer from QA , the primary bound quinone acceptor , to QB , the secondary bound quinone acceptor of PSII , resulted in a decrease in oxygen consumption rate at basic pH to value close to ones measured at pH 6.5 . - pubmed
In experiments with PSII preparations treated with Tris to destroy the water - oxidizing complex , photoconsumption of oxygen in the entire pH region was close to the values in untreated preparations at basic pH. - pubmed
In untreated preparations the rate of light - induced oxygen consumption decreased in the presence of catalase , which decomposes H2O2 , as well as in the presence of electron acceptor potassium ferricyanide . - pubmed
From these data it is suggested that the light - induced oxygen consumption in PSII is caused by two processes , by an interaction of O2 with organic radicals , which were formed due to oxidation of components of the donor side of this photosystem ( proteins , lipids , pigments ) by cation - radical P680 + , as well as by oxygen reduction by still unidentified components of PSII . - pubmed
A site of ERK - dependent phosphorylation on Smad2 was located to Thr(8 ) , a site that overlaps with the calmodulin binding region . - pubmed
We show that calmodulin inhibits Smad2 phosphorylation by ERK1 , and overexpressing calmodulin , or stimulating calmodulin activity with ionomycin , reduces Smad2 levels . - pubmed
Interestingly , one known IgE - binding epitope of Ara h 3 shares 67 % of homologous amino acid residues with the corresponding area of Cor a 9 . - pubmed
The amino acids that differ were previously shown not to be critical for IgE binding in Ara h 3 . - pubmed
Triterpenes and phytosterols as human leucocyte elastase inhibitors . - pubmed
Ten triterpenes and phytosterols beta - amyrin , lupeol , lupeol acetate , ursolic acid , friedelin , canophyllol , 29 - hydroxy - friedelan-3 - one , beta - sitosterol , 3 - O - beta - D - glucopyranosyl - beta - sitosterol , 3 - O -( 6 '- O - palmitoyl )- beta - D - glucopyranosyl - beta - sitosterol , were evaluated as potential inhibitors of human leucocyte elastase ( HLE ) . - pubmed
In this series , lupeol , ursolic acid and canophyllol showed marked HLE inhibitory activity with IC(50 ) values at 1.9 microM , 4.4 microM , and 2.5 microM , respectively . - pubmed
It appeared that HLE inhibition depended on the presence and the orientation of two reactive groups in the tested molecules , distant from 10 - 12 A , reacting with Arg-217 in S(4 ) -S(5 ) subsites of the extended substrate - binding domain of HLE , and S(3 ) , respectively . - pubmed
The significantly higher level of cholesterol in aging neuronal membrane , the slow rate of cholesterol turnover , and the decreased level of total polyunsaturated fatty acids ( PUFA ) may result from poor passage rate via the blood - brain barrier , or from a decreased rate of incorporation into the membrane , or a decrease in the activities of delta-6 and delta-9 desaturase enzymes . - pubmed
FGF2 affords neuroprotection by interfering with a number of signaling pathways , including expression and gating of NMDA receptors , maintenance of Ca2 + homeostasis and regulation of ROS detoxifying enzymes . - pubmed
This stable cell line was validated with known CYP1A1 inducers , such as 3 - methylcholanthrene ( 3 - MC ) , beta - naphthoflavone ( beta - NF ) , alpha - naphthoflavone ( alpha - NF ) and 3 - indocarbinol . - pubmed
RESULTS : CYP1A1 reporter gene system can be used to identify strong inducers , such as 3 - MC , beta - NF and alpha - NF , and weak inducers , such as 3 - indocarbinol . - pubmed
Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat - tolerance of photosystem II . - pubmed
The PSII core complex of hf-2 , as compared with that of the wild type , showed structural fragility when solubilized with a detergent , dodecyl beta - d - maltoside , suggesting that the physical properties of the PSII complex were altered by the loss of SQDG . - pubmed
On the other hand , exposure of the cells to 41 degrees C for 120 min in the dark decreased the PSII activity to 70 % and 50 % of the initial levels in the wild type and hf-2 , respectively , which implies that the PSII activity , in the absence of SQDG , becomes less stable under heat - stress conditions . - pubmed
These results suggest that PSII inactivated by heat recovers through some mechanism dependent on light , and that SQDG participates in functioning of the mechanism . - pubmed
The conformational disorder of PSII caused by the defect in SQDG might be correlated with the increased susceptibility of its activity to heat - stress . - pubmed
BACKGROUND : A prospective phase II study was performed to determine the feasibility , efficacy and safety of arterial hepatic infusion ( HAI ) using pirarubicin combined with intravenous chemotherapy . - pubmed
[ Brain phosphatases in thiobarbiturate anesthesia ; histochemical study ] . - pubmed
The peptide hormone relaxin , which attains high circulating levels during pregnancy , has been shown to depress small - bowel motility through a nitric oxide ( NO)-mediated mechanism . - pubmed
In the presence of the NO synthesis inhibitor l - NNA , relaxin was ineffective . - pubmed
Direct smooth muscle contractile responses were not influenced by relaxin or l - NNA . - pubmed
Further addition of relaxin to the bath medium did not influence EFS - induced responses , whereas l - NNA did . - pubmed
A significant loss of ESP activity paralleled the decrease in sulforaphane nitrile formation . - pubmed
METHODS AND RESULTS : Ex vivo and in vivo sensitivity of coronary MR thrombus imaging was tested by use of intracoronarily delivered Gd - DTPA - labeled fibrinogen thrombi ( n=6 ) . - pubmed
Fibrinogen thrombi : 5 of 6 intracoronarily delivered Gd - labeled fibrinogen clots ( approximately 250 micromol / L Gd ) were visible on MRI and subsequently confirmed by x - ray angiography . - pubmed
Curcumin sensitizes tumor necrosis factor - related apoptosis - inducing ligand ( TRAIL)-induced apoptosis through reactive oxygen species - mediated upregulation of death receptor 5 ( DR5 ) . - pubmed
This apoptosis induced by the combination of curcumin and TRAIL is not interrupted by Bcl-2 overexpression . - pubmed
We found that treatment with curcumin significantly induces death receptor 5 ( DR5 ) expression both at its mRNA and protein levels , accompanying the generation of the reactive oxygen species ( ROS ) . - pubmed
Not only the pretreatment with N - acetylcystine but also the ectopic expression of peroxiredoxin II , an antioxidative protein , dramatically inhibited the apoptosis induced by curcumin and TRAIL in combination , blocking the curcumin - mediated DR5 upregulation . - pubmed
Taken together , the present study demonstrates that curcumin enhances TRAIL - induced apoptosis by ROS - mediated DR5 upregulation . - pubmed
Part II : On the possible occurrence of Stereochemical Changes in Equilibrated Solutions of Reducing Sugars introduced into the Circulation . - pubmed
Characterization of hepatobiliary transport systems of a novel alpha4beta1 / alpha4beta7 dual antagonist , TR-14035 . - pubmed
PURPOSE : Our previous pharmacokinetic studies have demonstrated that TR-14035 , a novel dual antagonist for alpha4beta1 / alpha4beta7 integrin , selectively and strongly accumulated in the liver and was mainly excreted in bile as an unchanged drug . - pubmed
OATP1B1 / OATP - C and OATP1B3 / OATP8 expressed in oocytes mediated the TR-14035 uptake with K(m ) of 7.5 and 5.3 microM , respectively . - pubmed
OATP1B1 * 15 , a genetic variant of OATP1B1 , exhibited a decreased transport of TR-14035 compared with OATP1B1 * 1a . - pubmed
CONCLUSION : These results indicate that OATP1B1 and OATP1B3 are at least partly responsible for the accumulation of TR-14035 into hepatocytes , and Mrp2 principally mediates the biliary excretion of TR-14035 . - pubmed
Furthermore , genetic polymorphisms of OATP1B1 may cause an interindividual variability in the pharmacokinetics of TR-14035 . - pubmed
The use of postnatal dexamethasone was associated with shorter length , lower weight , lower weight for length and smaller head circumference ; this effect remained after correction for GA , BW and BW - SDS . - pubmed
Haloperidol is an inhibitor but not substrate for MDR1 / P - glycoprotein . - pubmed
The inhibitory effect of haloperidol on other MDR1 substrates was also investigated in terms of the optimization of haloperidol - based pharmacotherapy . - pubmed
The transepithelial transport of [ ( 3 ) H ] haloperidol did not differ between the two cell lines , and vinblastine , a typical MDR1 substrate , had no effect on the transport , suggesting that haloperidol is not a substrate for MDR1 , and it is unlikely that MDR function affects haloperidol absorption and brain distribution , and thereby the response to haloperidol . - pubmed
However , haloperidol was found to have an inhibitory effect on the MDR1 - mediated transport of [ ( 3 ) H ] digoxin and [ ( 3 ) H ] vinblastine with an IC50 value of 7.84+/-0.76 and 3.60+/-0.64 microM , respectively , suggesting that the intestinal absorption , not distribution into the brain , of MDR1 substrate drugs could be altered by the co - administration of haloperidol in the clinical setting , although further clinical studies are needed . - pubmed
Besides damage to several tissues in vivo , AZA-1 has been shown to cause cytotoxicity in a number of cell lines and alterations in actin cytoskeleton and cell morphology . - pubmed
The activation of caspases by AZA-1 was studied next using a fluorescent caspase inhibitor . - pubmed
Additionally , regarding the involvement of apoptosis / cytoskeleton signaling in AZA-1 - induced morphological effects , inhibition of caspases with Z - VAD - FMK did not affect AZA-1 - induced cytoskeletal changes , suggesting , together with the activation kinetics , that caspases are not responsible for AZA-1 - elicited morphological changes . - pubmed
Modulation of PKA , PKC , PI3 K , Erk , p38MAPK , glutathione and microtubules with inhibitors / activators did not inhibit AZA-1 - induced actin cytoskeleton rearrangement . - pubmed
The JNK inhibitor SP600125 seemed to slightly diminish AZA-1 effects , however due to the effects of the drug by itself the involvement of JNK in AZA-1 toxicity needs further investigation . - pubmed
Recently , we showed that repeated activation of NKT cells by their specific glycolipid ligand , alpha - galactosylceramide , leads to a change in function to an immune regulatory role with IL-10 production . - pubmed
Five important forms of nonallergic angioedema can be distinguished : hereditary ( HAE ) , acquired ( AAE ) , renin - angiotensin - aldosterone system ( RAAS)-blocker - induced ( RAE ) , pseudoallergic angioedema ( PAE ) and idiopathic angioedema ( IAE ) . - pubmed
Today , a plasma pool C1 - esterase inhibitor ( C1 - INH ) concentrate is the therapy of choice in HAE . - pubmed
Recently , several new drugs were developed : a recombinant C1 - INH , a kallikrein inhibitor ( ecallantide ) and a specific bradykinin - B2 - receptor antagonist ( icatibant ) . - pubmed
The authors examined potential neuroprotective effects in optic neuritis by SRT647 and SRT501 , two structurally and mechanistically distinct activators of SIRT1 , an enzyme involved in cellular stress resistance and survival . - pubmed
This neuroprotective effect was blocked by sirtinol , a SIRT1 inhibitor . - pubmed
, Chen , K. H.-C. , Chen , C.-L. , and Chan , M. K. ( 2007 ) Redox potentiometry studies of particulate methane monooxygenase : Support for a trinuclear copper cluster active site , Angew . - pubmed
10 Cu ( I ) ions cooperatively when cupric ions are added to the protein fragment in the presence of dithiothreitol or ascorbate . - pubmed
The propensity for the C - terminal subdomain to bind Cu ( I ) is consistent with the high redox potential(s ) determined for the E - cluster copper ions in the pMMO . - pubmed
These properties of the E - clusters are in accordance with the function proposed for these copper ions in the turnover cycle of the enzyme . - pubmed
Response to prednisone in relation to NR3C1 intron B polymorphisms in childhood nephrotic syndrome . - pubmed
In the SRNS group , we performed a three - marker haplotype analysis of NR3C1 in relation to the response to prednisone , represented as time to proteinuria resolution ( TPR ) as categorical and ordinal variable . - pubmed
The GTA haplotype was associated with a higher GC sensitivity , as determined by TPR , and was found to be more prevalent in early ( response & lt;or=7 days ) than late ( response & gt;7 days ) prednisone responders ( 27.7 vs. 14.5 % , hap - score = -2.22 , p = 0.05 adjusted for biopsy results ) . - pubmed
A mutational variant of the enzyme lacking the disulfide bond has been produced by substituting the two cysteine residues with alanine ( EcoAcP mutational variant C5A / C49A , mutEcoAcP ) . - pubmed
The wild - type protein folds according to a two - state model with a very fast rate constant ( k(F)(H2O)=72,600 s(-1 ) ) , while mutEcoAcP folds ca 1500 - fold slower , via the accumulation of a partially folded species . - pubmed
The correlation also holds for EcoAcP , provided the relative contact order value is recalculated by considering the disulfide bridge as an alternate path for the backbone to determine the shortest sequence separation between contacting residues . - pubmed
Sucrose gradients of serum revealed antibody complexes in both non - responders . - pubmed
A delta class GST from the same species shows marginal DDT - metabolizing activity but the activity of agGSTe2 is approximately 350x higher than the delta class agGST1 - 6 . - pubmed
To investigate its catalytic mechanism and the molecular basis of its unusually high DDT - metabolizing ability , three agGSTe2 crystal structures including one apo form and two binary complex forms with the co - factor glutathione ( GSH ) or the inhibitor S - hexylglutathione ( GTX ) have been solved with a resolution up to 1.4A. - pubmed
The binding of GSH or GTX does not induce significant conformational changes in the protein . - pubmed
The comparison with the less active agGST1 - 6 provides the structural evidence for its high DDT - detoxifying activity . - pubmed
In short , this is achieved through the inclination of the upper part of H4 helix ( H4 '' helix ) , which brings residues Arg112 , Glu116 , and Phe120 closer to the GSH - binding site resulting in a more efficient GS(-)-stabilizing hydrogen - bond - network and higher DDT - binding affinity . - pubmed
To identify genomic sequences associated with estrogen responsiveness , the 5'-promoter region ( -44 to +118 ) of the IGFBP-6 gene was cloned into a chloramphenicol acetyltransferase ( CAT ) reporter vector . - pubmed
Identification of the estrogen - responsive element ( ERE ) [ 5 - CCTTCA CCTG-3 ] ( -9 to +1 ) in this IGFBP-6 gene promoter region was confirmed using electromobility shift assays and deletion analysis . - pubmed
However , the spatiotemporal expression patterns of the AQP gene family during tubular maturation and the potential influence of genetic variation on these patterns and on water handling remain unknown . - pubmed
Furthermore , adult CD-1 kidneys expressed more AQP2 in the collecting ducts , which was reflected by a significant delay in excreting a water load . - pubmed
These data ( 1 ) substantiate the clustering of important genes during tubular maturation and ( 2 ) demonstrate that genetic variability influences the regulation of the AQP gene family during tubular maturation and water handling by the mature kidney . - pubmed
Helium decreased expression of the proinflammatory marker CD11b and ICAM-1 on leukocytes and attenuated the expression of the procoagulant markers CD42b and PSGL-1 on platelets . - pubmed
By making use of the aggregation - induced emission feature of compound 1 and the cascade reactions among acetylthiocholine iodide ( ATC ) , AChE , and compound 2 , a new fluorescence " turn - on " method is developed for AChE assay and inhibitor - screening . - pubmed
CTAB - extracted DNA was also purified using the Zymo ( DNA Clean & amp ; Concentrator 25 kit ) , Qtip 100 ( Qiagen Genomic - Tip 100 / G ) , and QIAEX II Gel Extraction Kit . - pubmed
A study of tungsten oxide nanowires self - organized on mica support . - pubmed
Self - organized straight nanowires of WO3 have been epitaxially grown on muscovite mica in low super - saturation conditions . - pubmed
The experimental results show that straight WO3 nanowires are orientated preferentially parallel to two of three high symmetry crystallographic directions of mica [ 100 ] and [ 110 ] or [ 100 ] and [ Formula : see text ] . - pubmed
The growth mechanism has been studied as a function of different preparation conditions with special focus on the role of potassium ions present on the mica surface . - pubmed
The final structural identification was performed by MS , ( 1 ) H - NMR and ( 13 ) C - NMR Spectra . - pubmed
Post - resuscitation NAC treatment also significantly attenuated the increase in cortical caspase-3 and lipid hydroperoxide concentrations . - pubmed
We report that in vitro pre - treatment of PBMC with sodium arsenite ( NaAs ) reduces IL2 secretion and T cell proliferation induced by PHA , but does not prevent expression of monocyte - derived cytokines ( IL1 , IL6 , TNFalpha ) functioning as lymphocyte - activating factors . - pubmed
Kinetic analysis showed that NaAs pre - treatment first inhibits , but thereafter markedly increases , induction of IL2 and IL2RA mRNA when T cells are stimulated with PHA for 8 h and 72 h , respectively . - pubmed
NaAs systematically and significantly up - regulated a set of 35 genes , including several immune and stress genes , such as IL13 , granulocyte - macrophage colony stimulating factor , lymphotoxin alpha and heme oxygenase-1 ( HO-1 ) . - pubmed
Up - regulation of HO-1 , a stress and immunosuppressive protein , was rapidly detectable , both in T cells and in PBMC treated with NaAs . - pubmed
Inhibition of the immunosuppressive activity of HO-1 in PBMC however failed to prevent NaAs - dependent inhibition of T cell proliferation induced by PHA . - pubmed
However , chronic THC increased T lymphocyte CXCR4 expression on both CD4 + and CD8 + T lymphocytes compared to control . - pubmed
The use of dexmedetomidine , an alpha - adrenergic receptor agonist , for sedation may reduce intensive care unit length of stay when compared with use of benzodiazepines . - pubmed
Using comprehensive gene expression analyses , we have identified selenoprotein P(SeP ) as a novel hepatokine that elevates plasma glucose levels by inducing insulin resistance . - pubmed
BACKGROUND : This is the first study to compare plasma and cerebrospinal fluid ( CSF ) pharmacokinetics of intravenous ( IV ) , oral ( PO ) , or rectal ( PR ) formulations of acetaminophen . - pubmed
Acetaminophen plasma and CSF levels were obtained at T0 , 0.25 , 0.5 , 0.75 , 1 , 2 , 3 , 4 , and 6 hours . - pubmed
RESULTS : IV acetaminophen showed earlier and higher plasma and CSF levels compared with PO or PR administration . - pubmed
Inhibition of forebrain mu - opioid receptor signaling by low concentrations of rimonabant does not require cannabinoid receptors and directly involves mu - opioid receptors . - pubmed
Increasing number of publications shows that cannabinoid receptor 1 ( CB ( 1 ) ) specific compounds might act in a CB(1 ) independent manner , including rimonabant , a potent CB ( 1 ) receptor antagonist . - pubmed
rimonabant treatment prior to that of NE treatment caused further attenuation on the maximal stimulation of Tyr - d - Ala - Gly -( NMe ) Phe - Gly - ol ( DAMGO ) , which is a highly specific MOR agonist . - pubmed
In radioligand competition binding assays we highlighted that rimonabant fails to displace effectively [ ( 3 ) H ] DAMGO from MOR in low concentrations and is highly unspecific on the receptor at high concentrations in CB ( 1 ) knockout forebrain and in their wild - type controls . - pubmed
Surprisingly , docking computational studies showed a favorable binding position of rimonabant to the inactive conformational state of MOR , indicating that rimonabant might behave as an antagonist at MOR . - pubmed
Estrogenic compounds were detected ( average of combined solids and liquid fractions ) at average concentrations of : 1.42 ng / L for estrone , 0.69 ng / L for 17beta estradiol , 3.83 ng / L for estriol ( E3 ) , 0.56 ng / L for 17alpha - ethynylestradiol , 64.2 ng / L for bisphenol A , 7.51 ng / L for 4 - nonylphenol and 5.93 ng / L for 4 - tert - octylphenol . - pubmed
Recombinant His - tagged PhpA requires Mn ( 2 + ) or Co ( 2 + ) for phosphatase activity , and shows strict specificity for phosphorylated tyrosine residues . - pubmed
The k(m ) values of PhpA for p - nitrophenyl phosphate ( pNPP ) and phosphotyrosine peptide , RRLIEDAEpYAARG , were 803 and 139 microM , respectively . - pubmed
The phosphatase activity of PhpA was inhibited by sodium orthovanadate with a k(i ) of 33 microM. - pubmed
A phpA mutant exhibited an elevated level of tyrosine phosphorylation of a 79 kDa protein and cytoplasmic tyrosine kinase , BtkA. - pubmed
The presence of intracellular dopamine can further enhance the relative abundance of aSynL transcripts through alternative polyadenylation site selection . - pubmed
High sensitivity of diffusion - weighted MR imaging for the detection of liver metastases from neuroendocrine tumors : comparison with T2 - weighted and dynamic gadolinium - enhanced MR imaging . - pubmed
PURPOSE : To compare the sensitivity and specificity of diffusion - weighted ( DW ) magnetic resonance ( MR ) imaging for identifying liver metastases from neuroendocrine tumor ( NET ) to those of T2 - weighted fast spin - echo ( FSE ) and three - dimensional dynamic gadolinium - enhanced MR imaging , with surgical and histopathologic findings as the reference standard . - pubmed
DW MR was more sensitive ( observer 1 : sensitivity , 71.6 % [ 116 of 162 ] , 95 % confidence interval [ CI ] : 64.2 % , 78.0 % ; observer 2 : sensitivity , 71.0 % [ 115 of 162 ] , 95 % CI : 63.6 % , 77.4 % ) than T2 - weighted FSE ( observer 1 : sensitivity , 55.6 % [ 90 of 162 ] , 95 % CI : 47.9 % , 63.0 % ; observer 2 : sensitivity , 55.6 % [ 90 of 162 ] , 95 % CI : 47.9 % , 63.0 % ) and dynamic gadolinium - enhanced MR ( observer 1 : sensitivity , 47.5 % [ 77 of 162 ] , 95 % CI : 34.0 % , 55.2 % ; observer 2 : sensitivity , 48.1 % [ 78 of 162 ] , 95 % CI : 40.6 % , 55.8 % ) ( P & lt ; .001 for both , McNemar test ) . - pubmed
The specificity of these sequences ranged from 88.9 % to 100 % ( DW MR vs T2 - weighted FSE MR : P & gt ; .99 , DW MR vs dynamic gadolinium - enhanced MR : P = .61 , and T2 - weighted FSE MR vs dynamic gadolinium - enhanced MR : P = .61 , McNemar test ) . - pubmed
CONCLUSION : DW MR imaging was more sensitive for the detection and characterization of liver metastases from NETs than T2 - weighted FSE and dynamic gadolinium - enhanced MR imaging and should be systematically performed . - pubmed
While the down - regulated four genes , MAPK4 , FASN , INSIG1 and SCD , were mainly linked to the endoplasmic reticulum and fatty acid biosynthesis , these two cell processes that are associated with drug sensitivity by increasing evidences . - pubmed
RESULTS : Overexpression of TGF - beta1 and phospho - Smad2 , and reduced expression of membrane beta - catenin , were closely associated with lung metastasis in ACC . - pubmed
CONCLUSION : TGF - beta1 , phospho - Smad2 and beta - catenin were significantly correlated with ACC metastasis . - pubmed
Changes of PCr , ATP , inorganic phosphate , and phosphomonoesters were measured by means of ( 31 ) Phosphorous - magnetic resonance spectroscopy during and post - exercise . - pubmed
RESULTS : Average PCr ( percentage of [ PCr ] at initial rest ( % [ PCr]i ) ) at the end of the exercise ( adults 17 + - 12 % [ PCr]i , children 38 + - 17 % [ PCr]i , P & lt ; 0.01 ) and recovery periods ( adults 37 + - 14 % [ PCr]i , children 57 + - 17 % [ PCr]i , P & lt ; 0.01 ) was significantly lower in adults compared to children , induced by a stronger PCr decrease during the first exercise interval ( adults -73 + - 10 % [ PCr]i , children -55 + - 15 % [ PCr]i , P & lt ; 0.01 ) . - pubmed
Glucose tolerance , insulin resistance , insulin secretory capacity , anthropometric measurements , lipid profiles , nutrient intakes and pregnancy outcomes were evaluated at 24 - 28 weeks of gestation . - pubmed
Characterization of CM572 , a Selective Irreversible Partial Agonist of the Sigma-2 Receptor with Antitumor Activity . - pubmed
Here , we characterize CM572 [ 3 -( 4 -( 4 -( 4 - fluorophenyl ) piperazin-1 - yl ) butyl )- 6 - isothiocyanatobenzo [ d ] oxazol-2 ( 3H )- one ] ( sigma-1 Ki & gt;= 10 microM , sigma-2 Ki = 14.6 + - 6.9 nM ) , a novel isothiocyanate derivative of the putative sigma-2 antagonist , SN79 [ 6 - acetyl-3 -( 4 -( 4 -( 4 - fluorophenyl ) piperazin-1 - yl ) butyl ) benzo [ d ] oxazol-2 ( 3H )- one ] . - pubmed
CM572 bound irreversibly to sigma-2 receptors by virtue of the isothiocyanate moiety but not to sigma-1 . - pubmed
Low concentrations of CM572 that were minimally effective were able to attenuate significantly the calcium signal and cell death induced by the sigma-2 agonist CB-64D [ ( + ) - 1R,5R -( E )- 8 - benzylidene-5 -( 3 - hydroxyphenyl )- 2 - methylmorphan-7 - one ] . - pubmed
Taken together , these data show that CM572 is a selective , irreversible sigma-2 receptor partial agonist . - pubmed
at 30mM NaCl , actual quantum efficiency of photosystem II values were 63 - 96 % of controls ) . - pubmed
In the present paper we wanted to investigate lungs exposed to 1 h of warm ischemia without heparin followed by flush - perfusion and cold storage compared to lungs harvested from heart beating donors ( HBD ) using standard harvesting technique . - pubmed
An Overview of Insulin Pumps and Glucose Sensors for the Generalist . - pubmed
Applying differential microscale stresses and strains showed that hyaluronic acid ( HA ) gels display semi - flexible polymer behavior , while laminin - rich ECM hydrogels display flexible polymer behavior . - pubmed
In this work , we developed a novel layered molybdenum disulfide ( MoS2 ) nanosheet peroxidase mimetic - based fluorescent catalytic biosensor for the sensitive and selective detection of Fe ( 2 + ) . - pubmed
To elucidate underlying mechanisms , we assessed the arrhythmogenic SR - Ca ( 2 + ) leak at the time of LVAD implantation ( HF - Im ) and heart transplantation ( HF - Tx ) and evaluated the effects of CaMKII - inhibition . - pubmed
In sum , CaMKII inhibition yielded a clear tendency toward a reduction of the SR - Ca ( 2 + ) leak ( n cells / patients = 76/6 vs. 108/6 , P = 0.08 ) . - pubmed
In HF - Tx , we detected an even higher CaSpF of 1.00 + - 0.10 100 mum(-1 ) x s(-1 ) and a higher SR - Ca ( 2 + ) leak compared with HF - Im ( increase by 81 + - 33 % , n cells / patients = 156/7 vs. 130/7 , P & lt ; 0.05 ) , which fits to the further decreased LV function . - pubmed
These data show that the SR - Ca ( 2 + ) leak correlates with the development of LV function after LVAD implantation and may represent an important pathomechanism . - pubmed
The fact that CaMKII inhibition reduces the SR - Ca ( 2 + ) leak in HF - Tx suggests that CaMKII inhibition may be a promising option to beneficially influence clinical course after LVAD implantation . - pubmed
Children with severe forms of malaria had significantly higher IgG to merozoite antigens ( all p & lt ; 0.05 ) , but not to VAR2CSA ( all p & gt ; 0.05 ) when compared to the healthy children . - pubmed
An organic imidazolium salt of terephthalic acid has been utilized as a linker precursor for the synthesis of an aluminum - based metal organic framework ( MOF ) with MIL-53(ht ) structure . - pubmed
Investigating Mutations to Reduce Huntingtin Aggregation by Increasing Htt - N - Terminal Stability and Weakening Interactions with PolyQ Domain . - pubmed
Huntington ' s disease is a fatal autosomal genetic disorder characterized by an expanded glutamine - coding CAG repeat sequence in the huntingtin ( Htt ) exon 1 gene . - pubmed
Competing models for the misfolding and aggregation phenomena have suggested the role of the Htt - N - terminal region and the CAG trinucleotide repeats ( polyQ domain ) in affecting aggregation propensities and misfolding . - pubmed
We investigate the mutation landscape of the Htt - N - terminal region and explore amino acid residue mutations that affect its structural stability and hydrophobic interactions with the polyQ domain . - pubmed
The serotonin transporter ( SERT ) and other monoamine transporters operate in either a forward transport mode where the transporter undergoes a full transport cycle or an exchange mode where the transporter seesaws through half - cycles . - pubmed
This prediction was verified by tryptic digestion of SERT - expressing membranes : in the absence of Na + , the N terminus was rapidly digested . - pubmed
However , amphetamine - induced efflux by SERT - DeltaN32 or SERT - DeltaN42 ( but not by SERT - DeltaN22 ) was markedly diminished . - pubmed
The findings are consistent with a model where the N terminus acts as a lever to support amphetamine - induced efflux by SERT . - pubmed
This potentially suggests involvement of CAT8 in xylose metabolism . - pubmed
Constructed cat8Delta strain isolated from wild strain showed diminished growth on glycerol , ethanol and xylose as well as diminished respiration on the last substrate . - pubmed
CAT8 deletant was characterized by activated transcription of genes XYL3 , DAS1 and RPE1 and slight increase in the activity of several enzymes involved in xylose metabolism and alcoholic fermentation . - pubmed
Ethanol production from xylose in cat8Delta mutants in the background of wild - type strain and the best available ethanol producer from xylose increased for 50 and 30 % , respectively . - pubmed
The maximal titer of ethanol during xylose fermentation was 12.5 g ethanol / L at 45 C. Deletion of CAT8 did not change ethanol production from glucose . - pubmed
Available data suggest on specific role of Cat8 in xylose alcoholic fermentation . - pubmed
Pingyangmycin Pretreatment Influences the Biological Behavior of Ocular Venous Malformation and Relates with Galectin-3 Expression . - pubmed
We conducted this study to investigate the effect of pingyangmycin pretreatment on the Gal-3 expressions and biological behavior of ocular venous malformation . - pubmed
RESULTS : Gal-3 protein was expressed in 35 ( 52 % ) of 67 samples in the nonpingyangmycin group and in 19 ( 28 % ) of 69 samples in the pingyangmycin group ( P & lt ; 0.05 ) . - pubmed
Gal-3 mRNA expression was detected in 39 ( 58 % ) of 67 samples in the nonpingyangmycin group and 22 ( 32 % ) of 69 samples in the pingyangmycin group ( P & lt ; 0.05 ) . - pubmed
The higher Gal-3 expressions were detected in samples with deeper invasiveness than those with superficial invasiveness before ( chi2 = 12.720 and 13.369 , respectively , both P & lt ; 0.05 ) and after pingyangmycin treatment ( chi2 = 8.429 and 4.590 , respectively , both P & lt ; 0.05 ) . - pubmed
Pingyangmycin treatment led to a significant difference in Gal-3 expressions at both protein and mRNA levels ( chi2 = 8.664 and 9.524 , respectively , both P & lt ; 0.05 ) . - pubmed
CONCLUSIONS : Gal-3 expression may be involved in the development and invasiveness of ocular venous malformation , and pingyangmycin can inhibit Gal-3 expression , indicating a role of pingyangmycin treatment before the removal of ocular venous malformation . - pubmed
The beta - galactoside binding protein galectin-7 ( gal-7 ) is constitutively expressed at abnormally high levels in the outside milieu and intracellular compartments of many types of epithelial cancer cells , most notably in aggressive forms of ovarian and breast cancer . - pubmed
The ADP purinergic receptor P2Y12 is mainly expressed by platelets with important clinical significance as a target for several clinically approved antithrombotic agents . - pubmed
Clopidogrel treated TSSC6 KO arterioles showed smaller and less stable thrombi with increased tendency to embolise in vivo . - pubmed
PURPOSE : Epidemiological information on the association between sugar - sweetened beverage ( SSB ) consumption and the risk for hypertension ( HTN ) in Koreans is very limited . - pubmed
Current therapy relies on administration of acetylcholinesterase inhibitors ( AChEIs ) -donepezil , rivastigmine , galantamine , and N - methyl - d - aspartate receptor antagonist memantine . - pubmed
In this review article , we made comprehensive literature survey emphasising on donepezil template which was structurally converted into plethora of MTLDs preserving anti - cholinesterase effect and , at the same time , escalating the anti - oxidant potential , which was reported as a crucial role in the pathogenesis of the Alzheimer ' s disease . - pubmed
Pyruvate carboxylase ( PC ) catalyzes the conversion of pyruvate to oxaloacetate ( OAA ) , an important metabolic reaction in a wide range of organisms . - pubmed
AIBP decreases atherogenesis by augmenting cholesterol efflux . - pubmed
We found that ISL administration reduced serum amylase and lipase levels and alleviated the histopathological manifestations of pancreatic tissue in a dose - dependent manner . - pubmed
Meanwhile , ISL decreased the oxidative stress injury and increased the protein expression of the Nrf2 / HO-1 pathway . - pubmed
Methods : In this randomized controlled trial , women with ovarian or endometrial cancer [ age : & gt;=19 y ; body mass index ( kg / m2 ): & gt;=18.5 ] were randomly assigned to a KD ( 70:25:5 energy from fat , protein , and carbohydrate ) or the American Cancer Society diet ( ACS ; high - fiber , low - fat ) . - pubmed
Body composition ( DXA ) and fasting serum insulin , IGF - I , and beta - hydroxybutyrate were obtained at baseline and at 12 wk ; urinary ketones were also measured throughout the intervention . - pubmed
The animals were treated with 20 mg / kg / day losartan , an antagonist of AT1 receptor , or captopril , an angiotensin - converting enzyme inhibitor . - pubmed
It was shown that the mechanism of cell death caused by BRET - induced phototoxicity of mimiSOG in the presence of furimazine depends on the intracellular localization of the NanoLuc - miniSOG fusion protein : BRET - mediated activation of miniSOG in mitochondrial localization causes apoptosis , while the membrane localization of PS causes necrosis of cancer cells . - pubmed
Fasting blood samples were obtained to analyze the serum concentration of bone turnover markers including osteocalcin ( OC ) , beta - isomerized type I collagen amino - terminal peptide ( beta - CTX ) , alkaline phosphatase ( ALP ) , type I procollagen amino - terminal peptides ( PINP ) , calcium , and phosphorus . - pubmed
CONCLUSIONS : These findings indicate the action of at least two different carotenoid cleavage dioxygenases ( CCD2 and tentatively CCD4 ) during biosynthesis of this new bis - apocarotenoid aglycon ( norcrocetin ) and the existence of even higher glycosylated crocin derivatives at trace level . - pubmed
The mevalonate pathway provides sterols for membrane structure and nonsterol intermediates for the post - translational modification and membrane anchorage of growth - related proteins , including the Ras , Rac , and Rho GTPase family . - pubmed
Mevalonate - derived products are also essential for the Hedgehog pathway , steroid hormone signaling , and the nuclear localization of Yes - associated protein and transcriptional co - activator with PDZ - binding motif , all of which playing roles in tumorigenesis and cancer stem cell function . - pubmed
The phosphatidylinositol-4,5 - bisphosphate 3 - kinase - AKT - mammalian target of rapamycin complex 1 pathway , p53 with gain - of - function mutation , and oncoprotein MYC upregulate the mevalonate pathway , whereas adenosine monophosphate - activated protein kinase and tumor suppressor protein RB are the downregulators . - pubmed
Sterol regulatory element binding protein 2 mediates the sterol - controlled transcriptional downregulation of HMGCR . - pubmed
UbiA prenyltransferase domain - containing protein-1 regulates the ubiquitination and proteasome - mediated degradation of HMGCR , which is accelerated by 24 , 25 - dihydrolanosterol and the diterpene geranylgeraniol . - pubmed
Statins , competitive inhibitors of HMGCR , deplete cells of mevalonate - derived intermediates and consequently inhibit cell proliferation and induce apoptosis . - pubmed
Tumor HMGCR is resistant to the sterol - mediated transcriptional control ; consequently , HMGCR is upregulated in cancers derived from adrenal gland , blood and lymph , brain , breast , colon , connective tissue , embryo , esophagus , liver , lung , ovary , pancreas , prostate , skin , and stomach . - pubmed
Nevertheless , tumor HMGCR remains sensitive to isoprenoid - mediated degradation . - pubmed
The contents of PFAAs and the FDOM in the water samples were measured by a high - performance liquid chromatograph - mass spectrometer ( HPLC - MS ) and by a fluorescence spectrophotometer , respectively . - pubmed
Compared to LH / FSH - secreting tumors , PRL - secreting tumors showed statistically significant decrease in the levels of N - acetylaspartate ( NAA ) , myo - inositol ( mI ) , scyllo - inositol ( sI ) , glycine , taurine , phosphoethanolamine ( PE ) and increase in the levels of glutamine . - pubmed
When compared with LH / FSH - secreting tumors , ACTH - secreting tumors showed statistically significant decrease in the levels of sI , glycine , PE and increase in the levels of aspartate . - pubmed
Although lipid extracts of PAs showed the presence of many common lipid molecules , only glycerophosphoethanolamine ( GPE ) showed statistically significant decrease in PRL , ACTH and non - functional subtypes when compared to LH / FSH - secreting tumors . - pubmed
After EA treatment , SAS and SDS scores were decreased in the acupuncture group ( P=0.007 and P=0.027 , respectively ) and were lower than those of the control group ( P=0.003 and P=0.004 , respectively ) . - pubmed
Hydroxymethylene diphosphonate for imaging transthyretin cardiac amyloidosis . - pubmed
RESULTS : T1 increased crude proteins , fat , Ca , Fe , vitamin C , total phenolic content , antioxidant capacity by DPPH and by ORAC . - pubmed
On the other hand , T1 decreased reducing sugars , K and Cu content . - pubmed
We measured their alpha - gal A activity and plasma/ serum globotriaosylsphingosine ( lyso - Gb3 ) accumulation and performed electron microscopy of skin biopsies . - pubmed
Multiple linear regression was carried out to construct an Immunity model ( adjusted R2 = 75.8 % ) comprising Macrophage chemotaxis and phagocytosis and Lymphoproliferation capacity , a Redox model ( adjusted R2 = 84.4 % ) involving Reduced Glutathione and Malondialdehyde concentrations and Glutathione Peroxidase activity and a Behavioural model ( adjusted R2 = 79.8 % ) comprising Internal Locomotion and Time spent in open arms indices . - pubmed
UCP2 Deficiency Increases Colon Tumorigenesis by Promoting Lipid Synthesis and Depleting NADPH for Antioxidant Defenses . - pubmed
Mechanistically , UCP2 loss increases levels of oxidized glutathione and proteins in tumors . - pubmed
UCP2 deficiency alters glycolytic pathways while promoting phospholipid synthesis , thereby limiting the availability of NADPH for buffering oxidative stress . - pubmed
Metformin attenuates PD - L1 expression through activating Hippo signaling pathway in colorectal cancer cells . - pubmed
Here , we showed that metformin decreases PD - L1 and YAP1 expression in vitro and in vivo . - pubmed
After silencing or inhibiting YAP1 expression by Verteporfin ( VP ) , the inhibitor of YAP1 , the expression of PD - L1 were decreased in protein level in CRC cells . - pubmed
Compared with the control group , PD - L1 and YAP1 expressions in tumor tissues , detected by immunohistochemistry , were reduced in the group of metformin treatment . - pubmed
In order to develop an affinity HPLC method for screening direct thrombin inhibitors from Traditional Chinese Medicine ( TCM ) , thrombin was immobilized on the glutaraldehyde - modified amino silica gel and was used as thrombin stationary phase . - pubmed
Thirty - two crowns were studied and divided into four groups ( n = 8) , according to the ceramic material ( feldspathic glass - ceramic ( F ) and zirconia reinforced lithium silicate glass - ceramic ( ZLS ) ) and to their technological obtaining processes ( milling ( M ) and heat - pressing ( P ) ) . - pubmed
AIMS : To conduct a systematic review and meta - analysis to assess the efficacy , safety and tolerability of sodium - glucose co - transporter-2 inhibitors vs placebo as add - on therapy after metformin and dipeptidyl peptidase-4 inhibitor dual therapy in type 2 diabetes . - pubmed
A search was conducted in the PubMed , www.clinicaltrials.gov and Cochrane Central Register of Controlled Trials databases for relevant randomized controlled trials up until 14 August 2020 that compared sodium - glucose co - transporter-2 inhibitors vs placebo as add - on therapy after metformin and dipeptidyl peptidase-4 inhibitor therapy . - pubmed
Compared with placebo , sodium - glucose co - transporter-2 inhibitor treatment , as add - on to metformin and dipeptidyl peptidase-4 inhibitor therapy , was associated with a significant reduction in HbA1c level [ mean difference -8 mmol / mol , 95 % CI -10 , -6 ( -0.7 % , 95 % CI -0.9 , -0.6 ) ; P < 0.00001 ] , in fasting plasma glucose level [ mean difference -1.70 mmol / l , 95 % CI -1.91 , -1.49 ; P < 0.00001 ] , in weight ( mean difference -1.76 kg , 95 % CI -2.04 , -1.48 ; P < 0.00001 ) and in blood pressure ( systolic blood pressure : mean difference -3.6 mmHg , 95 % CI -4.8 , -2.4 ; P<0.00001 ; diastolic blood pressure : mean difference -1.5 mmHg ; 95 % CI -2.4 , -0.6 ; P=0.002 ) . - pubmed
Maternal Prenatal Depression in Pregnancies With Female and Male Fetuses and Developmental Associations With C - reactive Protein and Cortisol . - pubmed
Maternal prenatal C - reactive protein and hair cortisol and cortisone levels were determined . - pubmed
Maternal cortisol levels were significantly associated with depression only in pregnancies with female fetuses and with decreased newborn P50 inhibition only in female newborns . - pubmed
In pregnancies with male fetuses compared with pregnancies with female fetuses , cortisol was more robustly metabolized to cortisone , which does not activate cortisol receptors . - pubmed
The successful use of PARP1 inhibitors like olaparib ( Loparza ) in the treatment of BRCA1 / 2 - deficient breast cancer has provided clinical proof of concept for applying personalized medicine based on synthetic lethality to the treatment of cancer . - pubmed
Unfortunately , all marketed PARP1 inhibitors act by competing with the cofactor NAD+ and resistance is already developing to this anti - cancer mechanism . - pubmed
identified 5F02 as an allosteric PARP inhibitor that acts by preventing the enzymatic activation of PARP1 by histone H4 . - pubmed
NMDAR antibody encephalitis overlapping with CLIPPERS syndrome in a psoriasis patient on adalimumab . - pubmed
Fractions eluting at different phosphate concentrations were analysed for the presence of EBNA by means of an anti - EBNA - specific 125I - labelled IgG absorption assay . - pubmed
Decreased fidelity of DNA polymerase activity during N-2 - fluorenylacetamide hepatocarcinogenesis . - pubmed
The fidelity of polymerization of these enzymes was compared by determining the incorporation of noncomplementary deoxyribonucleoside triphosphates ( misincorporation ) on a poly(dA - dT).poly ( dA - dT ) template , with MnCl2 and MgCl2 as divalent cations . - pubmed
The results indicate that alcohol dehydrogenase and aldehyde dehydrogenase do not share a common pool of NAD , and that NADH formed during acetaldehyde oxidation is utilized for reductions in the cytosol to a smaller extent than the NADH formed in the alcohol dehydrogenase reaction . - pubmed
Chemical modifications of spinach leaf nitrate reductase , and its 28,000 M(r ) fragment with phenylglyoxal , 2,3 - butanedione and pyridoxal phosphate reduce the catalytic activity of the enzyme . - pubmed
NADH protects the inactivation of NADH - ferricyanide reductase activity of the 28,000 M(r ) fragment of nitrate reductase . - pubmed
These data suggest that nitrate reductase contains active sites of arginine and lysine residues that are involved in the NADH binding site of the enzyme . - pubmed
The two agents evaluated were polylysine 111In - antimyosin ( 0.023 mg Fab modified with a 3.3 kd polymer of polylysine and labeled with 111In ) and 99mTc - antimyosin ( 0.5 mg Fab ' antimyosin labeled using the RP-1 ligand technique ) . - pubmed
Chronic blockade of the endogenous opioids with the long - acting opioid antagonist naltrexone could result in increased gonadotropin secretion and ovulation induction in these cases . - pubmed
The new oral third generation cephalosporins are effective in infections due to beta - lactamase - producing Haemophilus strains . - pubmed
Cell extracts of 3 - hydroxybenzoate - grown cells exhibited 3 - hydroxybenzoyl - CoA synthetase activity of 190 nmol min-1 mg protein-1 as well as benzoyl - CoA synthetase activity of 86 nmol min-1 mg protein-1 . - pubmed
Pertussis toxin pre - treatment only partially blocked presynaptic inhibition caused by noradrenaline but abolished the reduction of EPSP amplitude by somatostatin . - pubmed
The presynaptic inhibitory action of noradrenaline results from activation of alpha 2 - adrenoceptors at nerve terminals but the mechanism(s ) by which these presynaptic receptors act can not be explained adequately by either activation of a potassium conductance and/or inhibition of a calcium conductance . - pubmed
Plasma membrane sphingomyelin and the regulation of HMG - CoA reductase activity and cholesterol biosynthesis in cell cultures . - pubmed
We have examined the mechanism of the inhibition of cholesterol synthesis in cells treated with exogenous sphingomyelinase . - pubmed
Free fatty acid synthesis or general cellular protein synthesis was unaffected by the addition of sphingomyelinase . - pubmed
, ceramide and phosphocholine , had no effect on reductase activity . - pubmed
Sphingosine , a further product of ceramide metabolism , caused a stimulation of reductase activity . - pubmed
Examination of the incorporation of [ 3H ] acetate into the nonsaponifiable lipid fractions in the presence of sphingomyelinase showed no changes in the percent distribution of radioactivity in the post - mevalonate intermediates of the cholesterol biosynthetic pathway , but there was increased radioactivity associated with the polar sterol fraction . - pubmed
Pretreatment of cells with ketoconazole , a known inhibitor of oxysterol formation , prevented the inhibition of reductase activity by sphingomyelinase and decreased the incorporation of [ 3H ] acetate in the polar sterol fraction . - pubmed
Ketoconazole had no effect on exogenous sphingomyelinase activity in vitro in the presence or absence of cells . - pubmed
Endogenous sphingomyelinase activity was also unaffected by ketoconazole . - pubmed
, chlorpromazine , desipramine , and N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalene sulfonamide ( W-7 ) , to the culture medium caused a dose - dependent stimulation of reductase activity . - pubmed
Treatment of cells with small unilamellar vesicles of dioleyl phosphatidylcholine or high density lipoprotein3 resulted in increased efflux of cholesterol and stimulation of reductase activity . - pubmed
Under similar conditions , the inhibitory effect of exogenous sphingomyelinase on reductase activity was prevented by incubation with small unilamellar vesicles of phosphatidylcholine or high density lipoprotein . - pubmed
Cathepsin H splits off only one N - terminal amino acid from each tuftsin and enkephalin , which , according to the literature , led to inactivation of peptides . - pubmed
The enzyme acts on the oxidized B chain of insulin as an aminoendopeptidase : it splits off the N - terminal phenylalanine and the centrally located bond(s ) . - pubmed
Km and Vmax for the cathepsin H catalyzed hydrolysis of LeuNA , ArgNA LysNA and BANA were determined . - pubmed
Based on the data obtained and the previously reported results on conversion of kallidin into bradykinin , the specificity of cathepsin H and its possible biological functions were discussed . - pubmed
Cloned T cells or cells from lymphoid organs were stimulated with antigen , concanavalin A , or phorbol myristate acetate and ionomycin in microwells coated with antibodies specific for IFN - gamma . - pubmed
Similarly , using TNF - specific antibody reagents , TNF - secreting cells were detected and quantitated in cell populations obtained from normal lymphoid tissues , bone marrow and peripheral blood , following activation with phorbol myristate acetate and ionomycin . - pubmed
Effect of cetyltrimethylammonium on ATP hydrolysis and proton translocation in the F0 - F1 H +- ATP synthase of mitochondria . - pubmed
The action of opiate receptor agonists : ( D - Ala2 )- methionine enkephalinamide ( D - MEA ) , morphine , heroin , etorphine , and antagonists : naloxone and diprenorphine on audiogenic seizures was tested during ethanol abstinence . - pubmed
The first mechanism is renin independent , requires antecedent sodium retention and appears related to abnormal membrane transport of calcium . - pubmed
This vasoconstriction is identified by low plasma renin and ionized calcium and is correctable by sodium depletion or calcium channel or alpha blockade . - pubmed
The second vasoconstrictor mechanism is renin mediated and involves an increase in cytosolic calcium . - pubmed
This mechanism is quantifiable by the plasma renin level or the hypotensive response to an antirenin - system drug ( converting enzyme inhibitor , beta blocker or saralasin ) . - pubmed
In this study , the fate of the polar AFNs during early development of Tubifex embryos has been followed using rhodamine - phalloidin as a specific stain for F - actin . - pubmed
The aromatic azido group in anti - cancer drug design : application in the development of novel lipophilic dihydrofolate reductase inhibitors . - pubmed
Two properties of the aromatic azido group have been exploited in the design of a novel dihydrofolate reductase inhibitor -- lipophilicity and biotransformability . - pubmed
This study examines the effects of N alpha - acetyl - beta - endorphin on tissue ODC activity to determine whether post - translational acetylation of beta - endorphin at the N alpha - terminus affects its capability to alter ODC activity and , consequently , tissue development . - pubmed
Naloxone inhibited the stimulatory actions of N alpha - acetyl - beta - endorphin on brain ODC completely , indicating the involvement of opioid receptors in that process . - pubmed
On the other hand , the increases in liver ODC were not prevented by naloxone , suggesting that these effects are not mediated through opioid - sensitive structures. - pubmed
In addition , S -(+)-enantiomers of aporphines and dopaminergic antagonists are equally potent as inhibitors of this enzyme , as compared to the corresponding R -(-)-enantiomers and other aporphine agonists . - pubmed
These results suggest that the inhibitory effects of apomorphine and its analogs against this enzyme are not mediated by their stimulation of dopamine autoreceptors . - pubmed
Since dihydropteridine reductase is required in vivo for the hydroxylation of tyrosine , the inhibition of this enzyme by apomorphine may represent one of several mechanisms by which apomorphine inhibits catecholamine synthesis . - pubmed
We now report that pure CRBP delivers retinol to the specific nuclear binding sites without itself remaining bound . - pubmed
CRBP is also capable of delivering retinol specifically to isolated chromatin with no apparent loss of binding sites , as compared to whole nuclei . - pubmed
CRBP again does not remain bound after transferring retinol to the chromatin binding sites . - pubmed
When isolated nuclei are incubated with [ 3H ] retinol - CRBP , sectioned , and autoradiographed , specifically bound retinol is found distributed throughout the nuclei . - pubmed
Thus , CRBP delivers retinol to the interior of the nucleus , to specific binding sites which are primarily , if not solely , on the chromatin . - pubmed
This laminin binding is calcium - dependent and can be competed by heparin . - pubmed
Twenty - two thrombovascular accidents were seen ( 16 % , 13 during acetylsalicylic acid and 9 during placebo , NS ) , including 17 fistula thromboses . - pubmed
The synergistic effect of the combination of VIP with noradrenaline ( NA ) was antagonized by pretreatment with prazosin or phentolamine but not by pretreatment with yohimbine or propranolol . - pubmed
Distal accentuation of conduction slowing in polyneuropathy associated with antibodies to myelin - associated glycoprotein and sulphated glucuronyl paragloboside . - pubmed
With the use of the antioxidant alpha - tocopherol with essential 12 hours after the development of peritonitis no significant changes occurred in the LPO values . - pubmed
Ventilator pressures were limited to & lt ; or = 39/9 cm H2O in the CMV - HI group . - pubmed
Remission maintenance therapy with histamine and interleukin-2 in acute myelogenous leukaemia . - pubmed
Histamine , of concentrations within the micromolar range , abrogated the monocyte - induced inhibition of NK - cells ; thereby , histamine and IL-2 synergistically induced NK - cell - mediated killing of AML blasts . - pubmed
The effect of histamine was apparently mediated by H2 - type histamine receptors ( H2R ) , since the H2R antagonist ranitidine completely blocked the response . - pubmed
Toxicity was moderate and include local reactions to IL-2 at the site of injection and short - lasting flush , hypotension , and headache to histamine . - pubmed
The addition of histamine to treatment with IL-2 significantly enhanced the accumulation of CD25 + T cells in peripheral blood as compared to treatment with IL-2 alone ( P< ; 0.003 ) . - pubmed
The N - terminal segment which contains the first three disulfide bridges is similar to epidermal growth factor . - pubmed
An inactivating Asn - Ser mutation in this region showed the same 3D structure indicating that these three residues , and possibly an additional Tyr in an adjacent loop , provide direct contacts in the interaction with nidogen . - pubmed
This collagen is very insoluble and resistant to the action of depolymerizing agents ( 4 % EDTA - Na2 , pepsin ) . - pubmed
Thus , the vast majority of the oligonucleotides synthesized by the 71 core facilities participating in this study were of high quality and performed well as sequencing primers without post - synthesis purification or desalting . - pubmed
Finally , our results suggest that an increase in the standard minimum performance specifications of DNA synthesis instruments and reagents from & gt ; or = 98 % to & gt ; or = 98.5 % average coupling efficiency , or the development of rapid , inexpensive and efficient methods to detect syntheses below the 98.5 % threshold , could obviate post synthesis purification of sequencing primers . - pubmed
Acute effects of bromocriptine , cyproheptadine , and valproic acid on plasma adrenocorticotropin secretion in Nelson ' s syndrome . - pubmed
ACTH was measured every 20 min for 8 h , 2 h before and 6 h after each of the following six treatments : placebo , bromocriptine ( 2.5 mg ) , cyproheptadine ( 8 mg ) , valproic acid ( 1 g ) , cyproheptadine plus valproic acid , and the combination of all three drugs . - pubmed
The percent maximal suppression of ACTH after administration of placebo ( 6 + /- 11 % ) , cyproheptadine ( 17 + /- 15 % ) , valproic acid ( 37 + /- 10 % ) or the combination of cyproheptadine and valproic acid ( 19 + /- 14 % ) did not achieve statistical significance . - pubmed
Bromocriptine , on the other hand , caused a significant decrease in plasma ACTH ( 52 + /- 8 % ; P & lt ; 0.05 ) , as did the combination of bromocriptine , cyproheptadine , and valproic acid ( 58 + /- 12 % ; P & lt ; 0.05 ) . - pubmed
We conclude that at the doses studied , bromocriptine had the greatest acute effect in suppressing ACTH secretion in Nelson ' s syndrome , and that combined administration with valproic acid and cyproheptadine did not further increase this acute ACTH - suppressive effect . - pubmed
The adverse course of the disease could not be influenced by repeated haemodialysis nor by the administration of disodium pamidronate ( Aredia ) and calcitonin . - pubmed
The circular dichroism ( CD ) spectrum of the denatured state of barstar has been analyzed as a function of urea and temperature . - pubmed
Water - soluble 2,3,7,8 - tetrachlorodibenzo - p - dioxin complex with human alpha - fetoprotein : properties , toxicity in vivo and antitumor activity in vitro . - pubmed
TCDD has been shown to form a stable complex with AFP in a 2:1 ( TCDD : AFP ) ratio . - pubmed
AFP may facilitate TCDD transport in embryonic tissues and enhance its embryotoxic and teratogenic effects . - pubmed
The data that imply a role for BCL2 and related molecules in such processes are reviewed in this article , which analyzes the factors intrinsic to the neuron that control its capacity for axonal growth . - pubmed
We investigated several assays to identify and characterize compounds that interfere with the binding of zinc to E6 . - pubmed
METHODS : Thirty - six compounds were selected on the basis of their structure , which would facilitate their participation in sulfhydryl residue - specific redox reactions , and were tested for their ability to release zinc from E6 protein . - pubmed
The zinc - ejecting compounds were then tested for their ability to inhibit E6 binding to E6 - associated protein ( E6AP ) and E6 - binding protein ( E6BP ) , two coactivators of E6 - mediated cellular transformation . - pubmed
RESULTS : Nine of the 36 tested compounds ejected zinc from E6 . - pubmed
Aspirin , heparin , low - molecular - weight heparin , hirudin and GP - IIb / IIIa blockers ] . - pubmed
The use of GP - IIb / IIIa blockers , especially the chimeric antibody fragment abciximab , is well established in interventional cardiology . - pubmed
Higher - than - standard doses of mitoxantrone have been shown to augment responses to cytarabine , possibly by increasing the DNA strand breaks induced by topoisomerase II ; these strand breaks can not be effectively repaired in the presence of cytarabine triphosphate . - pubmed
Toxicity of PrP106 - 126 may thus be mediated via a sequestration pathway that is not effective for this peptide in PrP - null cells . - pubmed
Three of these sites are involved in binding C3b and regulating complement activation ; others bind to sialic acid and/or heparin and are responsible for host recognition . - pubmed
The correlation of gastric emptying and time factors ( age , duration of diabetes ) and metabolic factors ( blood sugar level , glycosylated haemoglobin ) . - pubmed
No significant relationship was found between metabolic parameters ( blood glucose level , glycosylated haemoglobin ) and the time of gastric emptying . - pubmed
The authors did not find a relationship with the blood glucose level or glycosylated haemoglobin . - pubmed
The characteristics of centenarians in Tokyo is 1 ) low level of nutritional parameters , 2 ) low level of cholesterol and HDL - cholesterol , 3 ) low level of red blood cells and hemoglobin , 4 ) high level of CRP , 5 ) high level of homocysteine , 6 ) high level of von Willbrand factor . - pubmed
The level of homocysteine is associated with the level of von Willbrand factor , suggesting that homocysteine is related to endothelial injury . - pubmed
The active site of metalloproteases is composed of a primary sphere of residues co - ordinating the zinc atom , and a secondary sphere of residues that determines proteolytic specificity and activity . - pubmed
Glu-261 and Glu-267 directly co - ordinates the zinc atom in BoNT / A and BoNT / B respectively and the corresponding residue of TeNT was replaced by Asp or by the non conservative residue Ala. - pubmed
Tyr-365 is 4.3A away from zinc in BoNT / A , and the corresponding residue of TeNT was replaced by Phe or by Ala. - pubmed
The proteolytic activity of TeNT - Asp-271 ( E271D ) is similar to that of the native molecule , whereas that of TeNT - Phe-375 ( Y375F ) is lower than the control . - pubmed
These results demonstrate that both Glu-271 and Tyr-375 are essential for the proteolytic activity of TeNT . - pubmed
AIM : Protein tyrosine phosphatase-1B ( PTP-1B ) is an intracellular PTP known to dephosphorylate and inactivate upstream tyrosine phosphoproteins in the insulin signalling cascade . - pubmed
The aim of the present work was to further elucidate the role of PTP-1B in glucose metabolism in vivo . - pubmed
A hyperinsulinaemic euglycaemic clamp was used to study whole body glucose disposal and endogenous glucose production rates . - pubmed
Seven days post injection , no significant difference in rate of weight gain , fasting blood glucose or insulin levels were seen in any group . - pubmed
Similarly , under steady - state glucose clamp conditions , glucose disposal rate ( R(d ) ) , endogenous glucose production rate ( EGP ) and serum insulin levels were similar in all groups . - pubmed
CONCLUSION : We conclude that moderate medium - term overabundance , to a degree resembling that seen in insulin - resistant states , of PTP-1B in liver tissue does not alter insulin action on glucose metabolism and that the major site of action of PTP-1B is presumably at insulin - responsive target tissue or tissues other than the liver . - pubmed
A glutamate residue at the C terminus regulates activity of inward rectifier K + channels : implication for Andersen ' s syndrome . - pubmed
We found that a glutamate residue of GIRK2 ( E315 ) , located on a hydrophobic domain of the C terminus , is crucial for the channel activation . - pubmed
This glutamate ( or aspartate ) residue is conserved in all members of the Kir family . - pubmed
The mutated GIRK1 and GIRK2 retained ion selectivity to K (+ ) ions . - pubmed
Methyl - coenzyme M reductase ( MCR ) catalyzes methane formation from methyl - coenzyme M ( methyl - SCoM ) and N-7 - mercaptoheptanoylthreonine phosphate ( CoBSH ) . - pubmed
The active form of MCR , the red1 state , is generated by reducing another spectroscopically distinct form called ox1 with titanium ( III ) citrate . - pubmed
Previous electron paramagnetic resonance ( EPR ) and ( 14 ) N electron nuclear double resonance ( ENDOR ) studies indicate that both the ox1 and red1 states are best described as formally Ni ( I ) species on the basis of the character of the orbital containing the spin in the two EPR - active species . - pubmed
RR spectra are also reported for isolated cofactor F(430 ) in the reduced , resting , and oxidized states ; selected RR data are reported for the ( 15 ) N and ( 64 ) Ni isotopomers of the cofactor , both in the intact enzyme and in solution . - pubmed
Small Ni K - edge energy shifts indicate that minimal electron density changes occur at the Ni center during redox cycling of the enzyme . - pubmed
Analyses of the XANES and EXAFS data reveal that both the ox1 and red1 forms are best described as hexacoordinate and that the main difference between ox1 and red1 is the absence of an axial thiolate ligand in the red1 state . - pubmed
Furthermore , the vibrational characteristics of the ox1 state and red1 states are significantly different , especially in hydrocorphin ring modes with appreciable C = N stretching character . - pubmed
It is proposed that these differences arise from a 2 - electron reduction of the hydrocorphin ring upon conversion to the red1 form . - pubmed
Presumably , the ring - reduction and ligand - exchange reactions reported herein underlie the enhanced activity of MCR(red1 ) , the only form of MCR that can react productively with the methyl group of methyl - SCoM. - pubmed
The fetal ductus can be constricted by drugs , including cyclooxygenase inhibitors ( indomethacin ) , nitric oxide synthesis antagonists [ N - nitro - L - arginine monomethyl ester ( L - NAME ) ] , and glucocorticoid hormones ( dexamethasone ) . - pubmed
Poorly metabolised phytanic acid ( PA ) , pristanic acid ( PrA ) and picolenic acid ( PiA ) accumulates in fatty tissues , myelin sheaths , heart , kidneys and retina , leading to retinitis pigmentosa , peripheral dissociative polyneuropathy , cerebellar ataxia ( " sailors " walk ) , renal , cardiac and liver impairment . - pubmed
nidulans hxtA gene was repressed under high glucose conditions and expressed in vegetative hyphae upon carbon starvation and during sexual development . - pubmed
We found hxtA ( p )::sgfp expression in developing cleistothecia specifically in ascogenous hyphae and propose that HxtA is a high affinity glucose transporter involved in sugar metabolism during sexual development . - pubmed
Paired biopsy samples were taken from the esophagus 3 cm above the gastroesophageal junction ; one biopsy was snap - frozen for measurement of IL-8 mRNA levels by real - time quantitative polymerase chain reaction , and another was formalin - fixed for histopathological evaluation . - pubmed
Lansoprazole treatment significantly reduced the IL-8 mRNA expression levels . - pubmed
Arsenic exposure significantly decreased the glutamate - pyruvate transaminase activity in kidney . - pubmed
Glucose 6 - phosphatase activity in liver tissue was also significantly decreased after arsenic treatment . - pubmed
In addition to these , liver lactate dehydrogenase activity was elevated due to arsenic treatment . - pubmed
Radioligand binding using [ ( 125 ) I ] cyanopindolol revealed that HF resulted in a comparable decrease in the density of beta1 - ARs in both surface and T - tubule sarcolemma ( 55+/-4 % , n=7 , P<0.001 ; and 45+/-10 % , n=7 , P<0.01 , respectively ) , but no significant change in beta2 - AR density was observed . - pubmed
Whole - cell patch clamp studies demonstrated a markedly blunted increase in I(Ca , L ) in response to saturating concentrations of the nonselective beta - AR agonist isoproterenol ( 0.1 micromol / L ) in failing myocytes compared with control ( 129+/-20 % , n=11 , versus 332+/-35 % , n=7 ; P<0.001 ) . - pubmed
Experiments testing beta1 - AR- and beta2 - AR - selective stimulation showed that the major component of the blunted response to nonselective beta - AR stimulation in HF was caused by beta2 - AR activation , resulting in a pertussis toxin - sensitive , Gi - mediated inhibition of the beta1 - AR - induced increase in I(Ca , L ) . - pubmed
In parallel , recent studies indicate that aromatase , the enzyme that converts testosterone to estradiol in the brain , is expressed in presynaptic terminals and modulated within minutes by Ca ( 2 +)-dependent phosphorylation . - pubmed
Levels of UDP - GlcNAc , and the subsequent addition of O - linked beta - N - acetylglucosamine ( O - GlcNAc ) to Ser / Thr residues , is involved in regulating nuclear and cytoplasmic proteins in a manner analogous to protein phosphorylation . - pubmed
Recent research has highlighted key roles for O - GlcNAc serving as a nutrient sensor in regulating insulin signaling , the cell cycle , and calcium handling , as well as the cellular stress response . - pubmed
[ From the Cochrane Library and Diabetes Care : self - monitoring of blood glucose probably an effective way to improve glycaemic control in patients with type 2 diabetes not taking insulin ] . - pubmed
Self - monitoring of blood glucose has been found to be effective for patients with type I diabetes and for patients with type 2 diabetes taking insulin . - pubmed
There is much debate on the effectiveness of self - monitoring of blood glucose in the management of patients with type 2 diabetes who are not taking insulin . - pubmed
Meanwhile , LK-4 ( 3 mg / kg ) significantly inhibited the elevation of plasma tumor necrosis factor - alpha , as well as values of GOT and GPT , and BUN and creatinine caused by LPS . - pubmed
The induction of inducible NO synthase and the overproduction of NO and superoxide anions by LPS were also reduced by LK-4 . - pubmed
Screening of several drugs by intradermal treatment prior to toxin injection demonstrated reduced ET - induced vascular leakage with a cyclo - oxygenase inhibitor ( indomethacin ) , agents that interfere with histamine ( pyrilamine or cromolyn ) , or a neurokinin antagonist ( spantide ) . - pubmed
Systemic administration of indomethacin or celecoxib ( cyclo - oxygenase inhibitors ) , pyrilamine , aprepitant ( a neurokinin 1 receptor antagonist ) , or indomethacin with pyrilamine significantly reduced vascular leakage associated with ET . - pubmed
Our results indicate that ET , acting indirectly or directly on a target yet to be identified , stimulates the production / release of multiple inflammatory mediators , specifically neurokinins , prostanoids , and histamine . - pubmed
We demonstrated that the HDACI combinations exert different effects on cell cycle arrest and differentiation as determined by nitro blue reduction and the expression of the early myeloid differentiation marker CD11b . - pubmed
The most beneficial effects were found by use of 6 - h pretreatment with PB and vitamin B3 before the exposition to RA alone or in combination with vitamin B3 , showing significant acceleration and a high level of granulocytic differentiation . - pubmed
This suggests that interrupting ET-1 interaction with its endothelin A ( ET ( A ) ) receptor could be useful for inhibiting prostate cancer bone metastasis and , as such , may enhance the therapeutic activity of docetaxel ( Taxotere ) , the most commonly used drug for the treatment of metastatic prostate cancer . - pubmed
Among 40 proteins identified , peroxiredoxin 2 ( Prx2 ) was oxidized and 58 kDa glucose - regulated protein ( GRP58 ) was phosphorylated after 5 min of Ang II ( 1 microM ) stimulation . - pubmed
Valsartan , a selective Ang II type 1 ( AT1 ) receptor blocker , and N - acetylcysteine , an antioxidant , inhibited both of these modifications , indicating the contribution of AT1 receptor and reactive oxygen species to oxidation of Prx2 and phosphorylation of GRP58 by Ang II . - pubmed
On the other hand , LOX metabolites are involved in production of progesterone , which is required for pregnancy maintenance . - pubmed
In this study , we evaluated the interaction between the LOX pathway , progesterone , and HSD11B2 . - pubmed
Specifically , we hypothesized that LOX metabolites would alter HSD11B2 and this effect would be mediated by progesterone . - pubmed
By radiometric conversion assay , real - time quantitative PCR , Western blot analysis , and ELISA , we examined HSD11B2 enzyme activity , HSD11B2 mRNA and HSD11B2 protein expression , and progesterone output . - pubmed
Net progesterone output was increased by LOX metabolites and decreased by LOX inhibitors . - pubmed
Progesterone down - regulated HSD11B2 activity and HSD11B2 and HSD11B2 expression , and these effects were blocked by RU486 . - pubmed
Furthermore , the suppressive effect of 12 - HETE on HSD11B2 activity was also reversed by RU486 . - pubmed
Aprepitan , a neurokinin-1 receptor antagonist , is a centrally active agent that might have the ability to decrease hot flashes . - pubmed
Furthermore , the mutation in TIP neither impaired the salicylic acid - mediated induction of HRT expression nor the enhanced resistance conferred by overexpression of HRT . - pubmed
Acetohydroxyacid synthase ( AHAS ; EC 2.2.1.6 ) is the first enzyme in the biosynthetic pathway of the branched - chain amino acids . - pubmed
AHAS requires the cofactors thiamine diphosphate ( ThDP ) , Mg ( 2 + ) and FAD for activity . - pubmed
AQP1 expression in TM cells was assessed by SDS - PAGE and Western blot using anti - AQP1 IgGs . - pubmed
Interestingly , MPs from ischemic muscles produced more reactive oxygen species and expressed significantly higher levels of NADPH oxidase p47 ( 6 - fold ; P<0.05 ) and p67 subunits ( 16 - fold ; P<0.001 ) than controls , whereas gp91 subunit expression was unchanged . - pubmed
We found that MnSOD activity was increased in adenocarcinoma , CG and HP tissues ( p<0.05 - 0.001 ) , while Cu / ZnSOD was significantly lower in adenocarcinoma and HP tissues ( p<0.001 ) when compared to the healthy control . - pubmed
The susceptibility of the face to hexyl nicotinate , sodium lauryl sulfate , and benzoic acid differed depending on location and age . - pubmed
carbonic anhydrase and nitrate reductase ) with an increasing concentration of Ni . - pubmed
However , an increase in proline content and the activities of catalase , peroxidase , and superoxide dismutase was observed as a result of an increase in Ni concentration . - pubmed
The toxic effects generated by Ni were ameliorated by HBL through an improved antioxidant system and osmolyte . - pubmed
CONCLUSIONS : Type , duration , and intensity of physical activity must be considered when planning carbohydrate replacement and insulin dose reduction , as must the timing of exercise . - pubmed
We investigated the associations of EPHX1 Tyr113His and His139Arg polymorphisms with asthma and wheezing outcomes , and focused on the functional genetic change in different ambient nitrogen dioxide ( NO2 ) levels on glutathione S - transferase p1 ( GSTP1 ) and glutathione S - transferase m1 ( GSTM1 ) genotypes . - pubmed
We examined the associations of EPHX1 Tyr113His and His139Arg genotypes and diplotypes with asthma and wheezing outcomes under different ambient NO2 exposures . - pubmed
RESULTS : Children with the EPHX1 Arg / His or Arg / Arg genotypes at codon 139 were significantly associated with increased risks of lifetime asthma ( adjusted OR [ aOR ] = 1.3 ; 95 % CI , 1.1 - 1.7 ; and aOR = 1.5 ; 95 % CI , 1.1 - 2.1 , respectively ) . - pubmed
The risk of EPHX1 139Arg allele and 113Tyr-139Arg diplotype were of greater magnitude in higher compared with lower NO2 communities . - pubmed
The increase of the effect from the EPHX1 139Arg allele with higher NO2 exposure was most marked in the GSTP1 Val allele and GSTM1 present genotype . - pubmed
Comparing and contrasting the ( 1 ) H -( 1 ) H correlation data obtained for both polymorphic forms and taking into account the single crystal structure data existing for the solid form CIP - SAC ( II ) was possible to extract some conclusions on the polymorph CIP - SAC ( I ) where no single crystal information is available . - pubmed
Optimized AR coatings for bulk crystalline Si and thin - film CuIn ( 1 - x ) GaxSe ( 2 ) ( CIGS ) solar cells as two representative cases are presented and the effect of solar spectrum in the AR coating designs is investigated . - pubmed
By incorporating the transparent conductive and buffer layers as part of the AR coating in CIGS solar cells ( 2mum - thick CIGS layer ) , a single MgF ( 2 ) layer could provide an average reflectance of 8.46 % for wavelengths ranging from 350 nm to 1200 nm and incident angles from 0 to 80 . - pubmed
RESULTS : Eight compounds were isolated , and their structures were elucidated as bauerenol ( I ) , myristic acid ( II ) , 11 - phenyl-1 -( 2 ' , 6 '- dihydroxy-4 '- methoxy - phenyl )- undecan-1 - one ( III ) , ardisinone A ( IV ) , ardisinone B ( V ) , ardisinone C ( VI ) , ardisinone F ( VI ) , beta - sitosterol ( VIII ) . - pubmed
We , therefore , conclude that an increase in MAP1S levels triggers autophagy to suppress genome instability such that both the incidence of diethylnitrosamine - induced hepatocarcinogenesis and malignant progression are suppressed . - pubmed
Hydrolysis of xylan with commercial xylanase caused breakdown into XOS comprising of xylobiose , xylotriose along with xylose . - pubmed
Response surface model ( RSM ) revealed highest xylobiose yield ( 11 g/100 g xylan ) at pH 5.03 , temperature 45.19 C , reaction time 10.11h with enzyme dose 17.41 U. - pubmed
Similarly for maximizing xylotriose yield , ideal hydrolysis conditions were pH 5.11 , temperature 40.33 C , reaction time 16.55 h with enzyme dose 13.20 U. - pubmed
This was confirmed when cytochrome c release was shown to be unaffected by the pan caspase inhibitor , z - VAD - fmk . - pubmed
Sulfated derivatives based on powdered cellulose were obtained , including those containing additional ( carboxymethyl , ethyl amide or phosphate ) groups , and their activity against blood clotting factors ( thrombin and Xa ) was studied . - pubmed
Lipid peroxidation and spectrin of RBC membrane in hydroxyurea treated Ebeta thalassaemia . - pubmed
The percentage of spectrin and the level of malondialdehyde ( MDA ) , fetal hemoglobin ( HbF ) , hemoglobin ( Hb ) and packed cell volume ( PCV ) were measured . - pubmed
The HbF level was significantly increased after 90 days of HU therapy . - pubmed
Different Ca2 + -sensitivities between the EF - hands of T- and L - plastins . - pubmed
Plastins are Ca ( 2 +)-regulated actin - bundling proteins , and essential for developing and stabilizing actin cytoskeletons . - pubmed
Using various spectroscopic methods , gel - filtration chromatography , and isothermal titration calorimetry , we here demonstrate that the EF - hand motifs of both T- and L - plastin change their structures in response to Ca ( 2 + ) , but the sensitivity to Ca ( 2 + ) is lower in T - plastin than in L - plastin . - pubmed
Using experimental approaches , including optical tweezer ( OT ) stretching and atomic force microscopy ( AFM ) indentation , we found that RBCs in the presence of C ( 60 ) NPs are softer than normal RBCs . - pubmed
The strain - stress relations of both normal and C ( 60 ) infiltrated RBC membranes are extracted from the data of AFM indentation , from which we proved that C ( 60 ) NP infiltration can affect the mechanical properties of RBC membrane and tend to weaken the tensile resistance of lipids bilayers . - pubmed
The influence of C ( 60 ) NP concentration on the mechanical properties of RBC membrane is successfully predicted . - pubmed
Insulin resistance and the metabolism of branched - chain amino acids . - pubmed
The BCAAs are particularly responsive to the inhibitory insulin action on amino acid release by skeletal muscle and their metabolism is profoundly altered in insulin resistant conditions and/or insulin deficiency . - pubmed
20 - Hydroxyecdysone - induced transcriptional activity of FoxO upregulates brummer and acid lipase-1 and promotes lipolysis in Bombyx fat body . - pubmed
In a previous study , we have shown that the molting hormone , 20 - hydroxyecdysone ( 20E ) , reduces insect food consumption resulting in fat body lipolysis during the non - feeding molting and pupation stages , and assumed that the transcription factor FoxO is involved in this process . - pubmed
Three new series of symmetric dimers containing a naphthoyloxybenzyl ( NB ) , benzoyloxynaphthyl ( BN ) , and naphthoyloxysalicyl ( NS ) mesogenic core linked to an alkylene spacer via an imino group were synthesized . - pubmed
The dimers having NB and BN mesogenic units display intercalated smectic structures regardless of the spacer parity . - pubmed
Great efforts have been undertaken in the last decades to develop new oximes to reactivate acetylcholinesterase inhibited by organophosphorus compounds ( OP ) . - pubmed
A repeated - measures ANOVA ( group factor : treatment group and control group ; time factor : initial visit , 1.5 , 3 , and 6 months ) was used to analyze the probing depth ( PD ) , CAL , bleeding on probing ( BOP ) , high - sensitivity C - reactive protein ( hsCRP ) , glycated hemoglobin ( HbA1c ) , and fasting plasma glucose . - pubmed
RESULTS : Significantly lower PD ( F=62.898 , P-0.000 ) , CAL ( F=51.263 , P-0.000 ) , BOP ( F=75.164 , P=0.000 ) , hsCRP ( F=6.391 , P=0.010 ) , HbA1c(F=4.536 , P=0.011 ) , and fasting plasma glucose level ( F= 3.073 , P=0.031 ) were observed after therapeutic periodontal improvement . - pubmed
Increasing occurrence was found in those formulae produced by the less complex manufacturing processes affecting casein / whey protein ratio : pre - term , 14.3 % ; starter , 35.3 % ; follow - up , 42.1 % ; toddler 87.6 % ; while hypoallergenic and lactose - free were totally exempt . - pubmed
Development of potent antagonists for formyl peptide receptor 1 based on Boc - Phe - D - Leu - Phe - D - Leu - Phe - OH . - pubmed
In this study , Boc - Phe - D - Leu - Phe - D - Leu - Phe - OH ( Boc - FlFlF ) , one of several FPR subtype selective antagonists , was chosen and the positions at the Phe residues were optimized . - pubmed
This work describes using phase - angle information from tapping - mode AFM to follow the interactions of hydrogen with palladium , polycarbonate , and iron . - pubmed
Real - time AFM phase - angle information allowed for the observation of multiphase mass transfer to and from the surface of palladium at atmospheric pressure and room temperature without the need for complex sample preparation . - pubmed
The AFM observations are quantitatively benchmarked against and confirm mass transfer predictions based on bulk hydrogen diffusion data . - pubmed
Each cell nucleus and cytoplasm were identified in relation to DAPI and CD99 , respectively , and protein biomarkers ( e.g. - pubmed
We performed additional testing in two genomic regions ( P2RX4 / P2RX7 and P2RY1 ) in a cohort from the Prevalence , Prediction , and Prevention of Diabetes in Botnia ( n = 3504 ) , which includes more refined measures of glucose homeostasis . - pubmed
Rosuvastatin significantly reduced the MPV and the lipid parameters including total cholesterol , triglyceride , and low - density lipoprotein cholesterol ( LDL - C ) . - pubmed
However , there was no correlation between MPV and LDL - C before ( r = -.66 ; P = .383 ) and after ( r = -.112 ; P = .135 ) rosuvastatin treatment or between DeltaMPV and DeltaLDL - C after 40 mg rosuvastatin daily therapy ( r = -.155 ; P = .073 ) . - pubmed
An alternative pathway involves the leukocyte heme protein myeloperoxidase , which catalyzes the oxidation of thiocyanate in the presence of hydrogen peroxide , producing isocyanate at inflammation sites . - pubmed
Recently , it has been shown that carbamylated low - density lipoprotein ( LDL ) induces endothelial dysfunction via lectin - like - oxidized LDL receptor-1 activation and increased reactive oxygen species production , leading to endothelial nitric oxide synthase uncoupling . - pubmed
Moreover , carbamylated LDL harbors atherogenic activities , including both binding to macrophage scavenger receptors inducing cholesterol accumulation and foam - cell formation , as well as promoting vascular smooth muscle proliferation . - pubmed
We have demonstrated that , in these nonerythroid cells , adult and embryonic- larval subdomains possess different DNase I sensitivities and different profiles of H3K27me3 , a histone modification introduced by PRC2 complex . - pubmed
They were randomly allocated to receive intravenous fosaprepitant 150 mg ( n = 19 ; NK1 group ) or ondansetron 4 mg ( n = 19 ; ONS group ) before general anesthesia . - pubmed
IGF-1 level was negatively correlated with body mass index , waist circumference ( WC ) , AHI , and sleep duration with oxygen ( O2 ) saturation & lt ; 90 % and positively correlated with the average and minimum O2 saturation ( p = 0.027 ) . - pubmed
In a multivariable linear regression , considering WC and minimum O2 saturation as independent variables , only the minimum O2 saturation was a predictor of low IGF-1 levels . - pubmed
Hemoglobin ( Hb)A1c correlated negatively with the minimum O2 saturation and IGF-1 levels . - pubmed
Therefore , we investigated the pattern of cerebral glucose metabolism , as well as dopamine transporter ( DAT ) availability in a large and clinically well - defined cohort . - pubmed
Testosterone and androgen receptor gene polymorphism are associated with confidence and competitiveness in men . - pubmed
We found that APN downregulated PTEN expression and that both it and an inhibitor of PTEN ( SF1670 ) increased the expression of osteogenic markers such as osteocalcin , alkaline phosphatase , and runt - related transcription factor-2 in APN - treated hBMSCs . - pubmed
Whether the specific blood transfer conductance for nitric oxide ( NO ) with hemoglobin ( thetaNO ) is finite or infinite is controversial but important in the calculation of alveolar capillary membrane conductance ( DmCO ) and pulmonary capillary blood volume ( VC ) from values of lung diffusing capacity for carbon monoxide ( DLCO ) and nitric oxide ( DLNO ) . - pubmed
To prospectively identify mechanisms through which HSP90 - dependent cancer cells evade pharmacologic HSP90 blockade , we generated multiple mutant KRAS - driven cancer cell lines with acquired resistance to the purine - scaffold HSP90 inhibitor PU - H71 . - pubmed
All cell lines retained dependence on HSP90 function , as evidenced by sensitivity to short hairpin RNA - mediated suppression of HSP90AA1 or HSP90AB1 ( also called HSP90alpha and HSP90beta , respectively ) , and exhibited two types of genomic alterations that interfere with the effects of PU - H71 on cell viability and proliferation : ( i ) a Y142N missense mutation in the ATP - binding domain of HSP90alpha that co - occurred with amplification of the HSP90AA1 locus , ( ii ) genomic amplification and overexpression of the ABCB1 gene encoding the MDR1 drug efflux pump . - pubmed
In support of a functional role for these alterations , exogenous expression of HSP90alpha Y142N conferred PU - H71 resistance to HSP90 - dependent cells , and pharmacologic MDR1 inhibition with tariquidar or lowering ABCB1 expression restored sensitivity to PU - H71 in ABCB1 - amplified cells . - pubmed
Finally , comparison with structurally distinct HSP90 inhibitors currently in clinical development revealed that PU - H71 resistance could be overcome , in part , by ganetespib ( also known as STA9090 ) but not tanespimycin ( also known as 17 - AAG ) . - pubmed
Comparison of everolimus- and biolimus - eluting coronary stents with everolimus - eluting bioresorbable vascular scaffolds : Two - year clinical outcomes of the EVERBIO II trial . - pubmed
We confirmed serine 14 as a target of MAPKs and proline - directed kinases like cyclin - dependent kinase 5 ( Cdk5 ) in cell - based as well as in vitro kinase assays and quantitative phosphoproteomic analysis of TRPC6 . - pubmed
These two factors - which peak for the best electron donor , the thiolate ligand - afford a slim and narrow barrier through which the H - atom can tunnel most effectively . - pubmed
BACKGROUND / AIMS : Peptidyl - prolyl cis - trans isomerase FKBP25 is a member of the FK506 - binding proteins family which has peptidyl - prolyl cis / trans isomerase domain . - pubmed
METHODS : The spatio - temporal changes in expression of endothelial FKBP25 upon oxygen - glucose deprivation ( OGD ) treatment were examined by Western blot and immunofluorescence . - pubmed
Intriguingly , FKBP25 nuclear translocation was further validated in peroxynitrite ( ONOO-)-treated endothelial cells . - pubmed
PFASs were analyzed in plasma using isotope - dilution ultra - pressure liquid chromatography coupled to tandem mass spectrometry ( UPLC - MS / MS ) . - pubmed
Regulation of Kv11.1 potassium channel C - terminal isoform expression by the RNA - binding proteins HuR and HuD. - pubmed
She was started on micafungin , a potent 1,3 - beta - D glucan synthase inhibitor , to broaden antimicrobial coverage when her insulin requirement decreased to zero for & gt;48 hours . - pubmed
Right after discontinuation of micafungin and her switch to a different antifungal , insulin requirements increased back to her baseline needs . - pubmed
Oral administration of linear 1,3 - beta - D glucan has been documented to decrease blood glucose levels significantly by inhibition of expression of sodium - glucose transporter 1 ( SGLT1 ) in intestinal mucosa . - pubmed
INTRODUCTION : Trebananib , a peptide - Fc fusion protein , blocks angiogenesis by inhibiting binding of angiopoietin-1 / 2 to the receptor tyrosine kinase Tie2 . - pubmed
Trebananib plus trastuzumab and paclitaxel was evaluated in human epidermal growth factor receptor 2 - positive breast cancer in an open - label phase 1b clinical study . - pubmed
CONCLUSIONS : This case indicated an association of BRCA2 mutation with high mutation loads and an excellent response of oxaliplatin - based chemotherapy regimen for LARC . - pubmed
Collectively , these results suggest that LSD2 regulates distinct sets of genes during brown and beige adipocyte formation.-Takase , R. , Hino , S. , Nagaoka , K. , Anan , K. , Kohrogi , K. , Araki , H. , Hino , Y. , Sakamoto , A. , Nicholson , T. B. , Chen , T. , Nakao , M. Lysine - specific demethylase-2 is distinctively involved in brown and beige adipogenic differentiation . - pubmed
A monosaccharide ( mainly glucose ) was also obtained from the enzymatic saccharification of thermal acid hydrolysate using 16 U / mL Celluclast 1.5 L enzyme at 45 C for 36 h. Detoxification was performed using the adsorption method with activated carbon , the overliming method with Ca ( OH ) 2 , and the ion exchange method with polyethyleneimine . - pubmed
We performed a randomized trial to assess whether the combination of NSAIDs and sublingual nitrate is more effective than NSAIDs alone in preventing PEP . - pubmed
This finding was further substantiated by varying Pb2 + loadings on carbon nanoparticles and overexpressing lnc - PCK1 - 2:1 . - pubmed
The ira2 isu1 double deletion led to strains with the highest specific xylose consumption- and ethanol production rates but also the lowest biomass titre . - pubmed
The signalling response revealed that ira2 isu1 changed the low glucose - signal in the background strain to a simultaneous signalling of high and low glucose , suggesting that engineering of the signalome can improve xylose utilization . - pubmed
Furthermore , the expression of beta - catenin , p - AKT , VEGF , MMP-2 and MMP-9 proteins could be down - regulated after SMA / CORM-2 treatment . - pubmed
SMA / CORM-2 exerted both in vitro and in vivo anti - proliferation and anti - metastatic effects , which may yield a novel therapeutic strategy for CRC . - pubmed
On the other hand , in MBL and DBF forests , the ambient dose rate decreased 10 - 20 % faster than that induced solely by physical decay of radiocesium for the observation period 2011 - 2016 . - pubmed
All formalin - fixed , paraffin - embedded blocks from the surgical specimens from each subject were sectioned and stained with hematoxylin and eosin and antibodies to tau ( Thermo Fisher Scientific Clone AT8 ) , and examined blindly for tau pathology , including lesions characteristic of CTE . - pubmed
Low - Fouling Electrosprayed Hemoglobin Nanoparticles with Antioxidant Protection as Promising Oxygen Carriers . - pubmed
Despite all the attempts to create advanced hemoglobin ( Hb)-based oxygen carriers ( HBOCs ) employing an encapsulation platform , major challenges including attaining a high Hb loading and long circulation times still need to be overcome . - pubmed
Finally , the Hb - NPs also preserve the reversible oxygen - binding and releasing properties of Hb . - pubmed
MicroRNA-21 expressions were evaluated using reverse transcription - polymerase chain reaction ( RT - PCR ) and osteogenesis was detected via Alizarin Red S ( ARS ) staining and measurement of alkaline phosphatase ( ALP ) activity . - pubmed
Sortase - Mediated Site - Specific Modification of Interleukin-2 for the Generation of a Tumor - Targeting Acetazolamide - Cytokine Conjugate . - pubmed
Site - specific conjugation between interleukin-2 and acetazolamide was obtained by sortase A - mediated transpeptidation . - pubmed
Optimization of manufacturability and tumor - targeting performance of acetazolamide - cytokine products will be required in order to enable industrial applications . - pubmed
METHODS : We induced increased O - GlcNAc by treating AD and control cybrid cell lines with Thiamet G ( TMG ) , an inhibitor of the O - GlcNAcase enzyme that mediates removal of the nutrient - dependent O - GlcNAc modification . - pubmed
At baseline , AD cybrid cell line mitochondria showed partial activation of several proteins that help maintain bioenergetic homeostasis such as AMP - Regulated Kinase suggesting that AD mitochondria initiate a state of nutrient stress promoting energetic compensation ; however , this compensation reduces the capacity of cells to respond to additional nutrient - related stresses such as TMG treatment . - pubmed
Also , TMG caused disruptions in acetylation and Sirtuin 3 expression , while lowing total energetic output of the cell . - pubmed
Ketogenic diet and ketone bodies enhance the anticancer effects of PD1 blockade . - pubmed
Supplementation of KD with sucrose ( which breaks ketogenesis , abolishing 3HB production ) or with a pharmacological antagonist of the 3HB receptor GPR109A abolished the antitumor effects . - pubmed
Mechanistically , 3HB prevented the ICB - linked upregulation of PD - L1 on myeloid cells while favoring the expansion of CXCR3 + T cells . - pubmed
Methylene Blue ( MB ) was used as an organic model compound . - pubmed
Breakage of the aromatic ring of MB appeared by the scission of the double C - S + C bond as a result of the attack of OH species on adsorbed and electronically reorganized MB molecule . - pubmed
Previous studies have found that tomatidine decreases hepatic lipid accumulation via regulation of vitamin D receptor and activation of AMP - activated protein kinase ( AMPK ) phosphorylation . - pubmed
In vivo and in vitro , tomatidine significantly suppressed the expression of fatty acid synthase and transcription factors involved in lipogenesis , and increased the expression of adipose triglyceride lipase . - pubmed
Tomatidine promoted the sirtuin 1 ( sirt1)/AMPK signaling pathway to increase lipolysis and beta - oxidation in fatty liver cells . - pubmed
These findings suggest that tomatidine potentially ameliorates obesity and acts against hepatic steatosis by regulating lipogenesis and the sirt1 / AMPK pathway . - pubmed
While pyruvate dehydrogenase was inhibited in vitro , halofenate also inhibited the activety of various other isolated enzymes . - pubmed
Role of endogenous somatostatin in the secretion of parathyroid hormone and calcitonin . - pubmed
Our previous in vitro and in vivo studies demonstrated that exogenous somatostatin inhibited secretion of both parathyroid hormone ( PTH ) and calcitonin ( CT ) . - pubmed
This study evaluates the possible role of endogenous somatostatin in PTH and CT secretion . - pubmed
These combined observations strongly suggest that endogenous somatostatin must have a suppressive effect on PTH and CT secretion . - pubmed
These data strongly suggest that somatostatin is a locally - synthesized hormone that has a role in modulation of both PTH and CT secretion . - pubmed
Experiments in vivo indicate that N - acetylation may be a possible alternative metabolic pathway for biogenic amines and that brain N - acetyltransferase activity may be regulated by a beta - adrenergic mechanism . - pubmed
These results suggest that potassium ion may function on the affinities of dihydropteroate and L - glutamate to the enzyme . - pubmed
Dihydrofolate synthetase was inhibited by the addition of reduced forms of homopteroic acid . - pubmed
When atherogenic indices ( such as the relationship between total cholesterol and HDL - cholesterol or the Apo A1 / apo B quotient ) are used , 20 to 30 % of an IDDM population considered to be in clinically acceptable control have to be considered pathological . - pubmed
This observation is even more important since the recent Diabetes Control and Complications Trial has shown that , especially in the younger group of patients , significantly higher total cholesterol and triglycerides and lower HDL - cholesterol were observed . - pubmed
In this work , the helix - forming residues in fragments of several proteins ( ribonuclease , thermolysin , tendamistat and angiogenin ) were identified by NOE and the helix proton shifts were measured as delta changes associated with helix - population increments driven by trifluoroethanol addition . - pubmed
Aromatic residues considerably distorted the periodicity of the helix amide shifts in some peptides , as evidenced by the delta shifts of a RNase A fragment 1 - 15 analog in which the two aromatic residues were replaced by Ala. - pubmed
The benzodiazepine receptor antagonist ZK 93426 blocks the capacity of amitriptyline to augment the uptake of 36Cl- by 30 microM GABA . - pubmed
Our results support a model whereby dorso - ventral mesodermal patterning can be attained by a single mesoderm inducing agent , possibly bFGF , which is uniformly distributed across the prospective dorsal - ventral axis , and which acts in concert with a dorsally localized signal , possibly a Wnt protein , which either alters the response of ectoderm to induction or modifies the character of mesoderm after its induction . - pubmed
Peptide histidine valine ( PHV ) is a 42 amino acid polypeptide closely related to the neuropeptides VIP , PHI and PHM . - pubmed
The lack of effect of PHV infusion on blood pressure , despite tachycardia and marked cutaneous vasodilatation , implies that PHV has a different effect on the resistance vessels from that of other peptides such as VIP . - pubmed
Receptors , growth factors and steroid insensitivity of tumours . - pubmed
Throughout the physiologic range of plasma insulin concentrations , both the increase in total body glucose uptake and the suppression of hepatic glucose production ( HGP ) were significantly impaired in the obese group ( P less than .001 to .01 ) . - pubmed
At the two highest plasma insulin concentrations , inhibition of HGP and the stimulation of glucose disposal were similar in both the obese and control groups . - pubmed
One high - affinity site is the common DM1 / sigma 1 site , the affinity of which is allosterically increased 4 to 5 - fold by 10 microM ropizine . - pubmed
The Kd values of the DM1 / sigma 1 for DM and ( + ) - 3 - PPP are 17 and 11 nM respectively . - pubmed
DM binds to the second high - affinity site ( R2 ) with a Kd of 15 nM ; this site has low affinity for ( + ) - 3 - PPP . - pubmed
Conversely , ( + ) - 3 - PPP binds with high affinity ( Kd 53 nM ) to another site ( R3 ) , that has low - affinity for DM . - pubmed
The cells were stained with monoclonal antibodies to ICP4 , ICP5 , ICP6 , ICP8 , and gB , using the avidin - biotin - peroxidase complex ( ABC ) method . - pubmed
[ The effect of the beta 1 selective beta blocker , betaxolol , on metabolism in type II diabetics ] . - pubmed
No significant alterations were observed in postprandial blood glucose , glycosylated haemoglobin and 24 - h urinary glucose excretion . - pubmed
The lack of noticeable influence on the carbohydrate metabolism during 4 - week therapy is presumably related to the high beta 1 - selectivity of betaxolol . - pubmed
No correlation between ILS - induced paroxysmal activity and a decrease in TTP contents was found . - pubmed
Na +/ K +- ATPase activity was determined by measuring the inorganic phosphate ( Pi ) released by incubation in a reaction medium in the presence and absence of K ions or ouabain . - pubmed
Fasting total plasma cholesterol , triglycerides , high density lipoprotein cholesterol ( HDL ) , and body mass index were also measured . - pubmed
Estimates were similar for heritability of HDL cholesterol ( 51 vs. 45 % ) . - pubmed
No common household effect ( except for adjusted HDL cholesterol ( 24 % ) , p less than 0.01 ) was statistically significant for the lipids , blood pressures , or body mass index . - pubmed
Spouse correlations for lipids , blood pressures , and body mass index were low , with a maximum of 0.12 ( p less than 0.05 ) for HDL cholesterol . - pubmed
blocked both acetylcholine- and neurokinin B- , but not substance P- or neurokinin A - induced salivation . - pubmed
, a putative substance P antagonist , reduced responses to mammalian neurokinins but caused a 2 - fold potentiation of acetylcholine - induced salivation . - pubmed
, a novel putative neurokinin B antagonist , significantly reduced substance P- and neurokinin B- but not acetylcholine- or neurokinin A - induced salivation . - pubmed
Liver microsomal inactivation of 4 - methyl-5 - amino-1 - formylisoquinoline thiosemicarbazone as an inhibitor of ribonucleotide reductase . - pubmed
Studies were carried out to determine the effects of preincubation of 4 - methyl-5 - amino-1 - formylisoquinoline thiosemicarbazone ( MAIQ ) with hepatic microsomes on the ability of MAIQ to inhibit CDP reductase activity in vitro . - pubmed
When MAIQ ( 5 microM ) was first incubated in the presence of hepatic microsomes and NADPH , CDP reductase activity was inhibited only 30 % . - pubmed
This attenuation of MAIQ inhibition was dependent on time of incubation and microsomal protein concentration and showed an obligatory requirement for NADPH or NADH . - pubmed
In addition , a significant positive correlation was found between serum testosterone levels and LH B / I ratios in the impotent men ( r = 0.45 ; P = 0.029 ) . - pubmed
This paper reports the results of administration of a course of three 1 ml intramuscular injections of anti - HBs immunoglobulin to the babies of HBsAg positive mothers recognised during pregnancy over a period 1982 - 1985 . - pubmed
However , when comparing measurements made during mexiletine treatment with those at baseline , mexiletine monotherapy increased only the refractory periods of S4 . - pubmed
The effective refractory period of S4 during mexiletine monotherapy ( 200 + /- 20 ms ) was significantly longer than at baseline ( 160 + /- 21 ms ) . - pubmed
The effective refractory period of S4 during combination therapy ( 253 + /- 26 ms ) was significantly longer than that of quinidine monotherapy ( 223 + /- 27 ms). - pubmed
Phorbol myristate acetate ( PMA ) suppresses polarization and locomotion and alters F - actin content of Walker carcinosarcoma cells . - pubmed
Changes in shape and chemokinesis of Walker carcinosarcoma cells were associated with alterations in the relative amount and the topographical distribution of F - actin as determined by NBD - phallacidin binding . - pubmed
Suppression by PMA was associated with loss of the polar topographical distribution of F - actin visualized by NBD - phallacidin binding . - pubmed
In the presence of PMA , the relative amount of F - actin was higher than in unstimulated controls and lower in cells exposed to microtubule - disassembling agents . - pubmed
1,4 - Bis [ 2 -( 3,5 - dichloropyridyloxy )] benzene ( TCPOBOP ) and related compounds as inducers of hepatic monooxygenases . - pubmed
Ten analogs of TCPOBOP have been synthesized and their activities as microsomal enzyme inducers evaluated . - pubmed
The results illustrate that changes in the structure of the pyridyloxy ring markedly affect enzyme induction activity . - pubmed
In addition , the effects of altered substitution pattern of the benzene ring and structural alterations of the internal ring moiety were evaluated by measuring hepatic microsomal coumarin hydroxylase activity . - pubmed
The results confirm the microsomal monooxygenase enzyme induction activity of TCPOBOP , and the observed structure - dependent potencies of several related homologs support a receptor - mediated mechanism of action for the process . - pubmed
Subcellular localization of the adrenal desmolase active in the formation of dehydroepiandrosterone ( 3 - hydroxy-5 - androsten-17 - one ) . - pubmed
A synthetic dodecapeptide corresponding to the predicted reading frame of the repeating nucleotide unit behaved , in an immunoradiometric assay , identically with the native P. - pubmed
Circulating angiotensin II and adrenal receptors after nephrectomy . - pubmed
Mineralocorticoid secretion is predominantly controlled by the octapeptide angiotensin II , which exerts trophic actions on the adrenal glomerulosa and acute regulatory effects on aldosterone biosynthesis . - pubmed
The trophic actions include stimulation of angiotensin II receptors and enzymes of the aldosterone biosynthetic pathway , with corresponding enhancement of the aldosterone secretory capacity of the adrenal gland . - pubmed
The positive regulatory action of angiotensin II on its adrenal receptors occurs with elevations of the circulating peptide concentration within the physiological range and probably contributes to the increased sensitivity of the adrenal during sodium deficiency . - pubmed
To clarify these conflicting views we evaluated the effects of nephrectomy on adrenal angiotensin II receptors in relation to blood angiotensin II and plasma electrolyte levels . - pubmed
Maltose at a concentration of 0.5 % stimulated the glucoamylase synthesis , but at a concentration of 4 % it repressed not only the enzyme synthesis but the growth of the parental strain on the agar medium . - pubmed
The mutant 66 produced about 2.9 times more glucoamylase than its parent when maltose was added at 0.5 % concentration to the medium . - pubmed
The addition of cAMP ( 5.10(-5 ] resulted in a partial release of maltose ( 4 % ) repression of the glucoamylase synthesis in both strains . - pubmed
These results suggest that thyroid hormone influences the cholesterol esterification through the activation of lecithin : cholesterol acyltransferase . - pubmed
Kinetic modeling indicates that these NMDA receptors open at a rate of 230 s-1 and close at a rate of 167 s-1 , that in the absence of desensitization the maximum probability of being open is approximately 0.5 , and that this probability is half - maximal at approximately 150 microM NMDA . - pubmed
The results indicate that heparin and fucoidan , a naturally occurring sulfated L - fucose polymer , suppress the formation of an initial non - covalent interaction between 125I - TGF - beta 1 and activated alpha 2 - M. - pubmed
Electrophoresis of 125I - TGF - beta 1 showed that fucoidan protects TGF - beta 1 from proteolytic degradation by plasmin and trypsin . - pubmed
While plasmin caused little , if any , activation of latent TGF - beta derived from vascular smooth muscle cells ( SMC ) , plasmin degraded acid - activated TGF - beta , and purified TGF - beta 1 , and this degradation was inhibited by fucoidan . - pubmed
In vitro , heparin and fucoidan tripled the half - life of 125I - TGF - beta 1 and doubled the amount of cell - associated 125I - TGF - beta 1 . - pubmed
p35 , a major antigen extracted from the membrane of this mycoplasma using Triton X-114 has been found to be a lipoprotein . - pubmed
Propranolol increased the density of cerebral beta - adrenoceptors by 77 % and reduced the downregulation induced by stress . - pubmed
Atenolol also increased the density of cerebral beta - adrenoceptors by 34 % and abolished the stress - induced downregulation in cerebral beta - adrenoceptor density . - pubmed
These results suggest that the antihypertensive action of propranolol and atenolol may be partly associated with the inhibition of stress - activated central beta - adrenoceptor transmission . - pubmed
The effect of the concentration of peptone , as an additive , was investigated on cell growth , monoclonal antibody formation , and cell metabolism over 0 - 10 g l-1 range . - pubmed
It was found that 1 - 5 g l-1 peptone can significantly promote the growth of cells and increase the formation of monoclonal antibody , especially at 3 - 5 g l-1 , when both the accumulating level and secretion rate of monoclonal antibody are higher than that at other peptone concentrations . - pubmed
The cell growth and monoclonal antibody formation were inhibited at higher peptone concentrations , e.g. - pubmed
The proteolytic activity of the enzyme against dimethyl casein was inhibited by ethylenediaminetetraacetic acid and alpha 2 - macroglobulin . - pubmed
Demonstration of the suitability of CGP 12177 for in vivo studies of beta - adrenoceptors . - pubmed
Injection of ( + /- ) -propranolol at 15 min displaced ~ 60 % of the radioactivity by 90 min indicating that the tracer binds to beta - adrenoceptor sites in vivo.6 . - pubmed
The results show that ( -)-[ 3H ]- CGP 12177 is a suitable ligand for assessing beta - adrenoceptors in vivo . - pubmed
Because somatostatin also prevents lymphocyte proliferation , we hypothesized that iIELs may influence production of somatostatin . - pubmed
Concentration maps of paramagnetic tracers Gd3 + were measured in time through direct measurements of spin lattice relaxation time T1 obtained by using a two - point stimulated echo pulse sequence . - pubmed
Based upon known morphology , we suggest that the only structure supporting triad position is the SR itself , and that a non - homogeneous distribution of the SR within the sarcomere might be responsible for maintaining triad location near the mid - region of the thin filaments . - pubmed
Determination of 5 - hydroxytryptophol in urine by high - performance liquid chromatography : application of a new post - column derivatization method with fluorometric detection . - pubmed
The eluate was injected onto an isocratically eluted C18 reversed - phase column and 5HTOL was converted into a fluorescent oxazole derivative by on - line post - column reaction with benzylamine in the presence of potassium hexacyanoferrate ( III ) . - pubmed
Removal of adenosine decreases the responsiveness of muscle glucose transport to insulin and contractions . - pubmed
In the heart and in adipocytes , adenosine potentiates insulin - stimulated glucose transport . - pubmed
There is controversy regarding the effect of adenosine in skeletal muscle , with reports of both an inhibitory effect and no effect , on insulin - stimulated glucose transport . - pubmed
Measurement of the increase in GLUT4 at the cell surface in response to a maximally effective insulin stimulus in the epitrochlearis muscle , using the exofacial label ATB -[ 3H ] BMPA , showed that adenosine deaminase treatment markedly reduces cell - surface GLUT4 labeling . - pubmed
The reduction in cell - surface GLUT4 labeling was similar in magnitude to the decrease in maximally insulin - stimulated glucose transport activity in adenosine deaminase - treated muscles . - pubmed
These results show that adenosine potentiates insulin- and contraction - stimulated glucose transport in skeletal muscle by enhancing the increase in GLUT4 at the cell surface and raise the possibility that decreased adenosine production or action could play a causative role in insulin resistance . - pubmed
The effects of intraarticular bupivacaine and adrenaline , and a comparison of synovial fluid cell and synovial membrane cytokine expression , were also investigated . - pubmed
The xanthone 6 - hydoxylases are cytochrome P450 monooxygenases , as shown by their dependence on NADPH and molecular oxygen and their inhibition by carbon monoxide and typical P450 inhibitors . - pubmed
In both cell cultures , xanthone accumulation was preceded by an increase in xanthone 6 - hydroxylase activity . - pubmed
Cells were activated by phorbol myristate acetate ( PMA ) and anti - CD3 , PMA - ionomycin , or lipopolysaccharide ( LPS ) and assessed for cytokine mRNA transcription by semiquantitative reverse transcriptase - polymerase chain reaction , intracellular protein production by flow cytometry , and secretion by supernatant ELISA . - pubmed
CF cells secreted less IFN - gamma than control cells after maximal activation by PMA - anti - CD3 ( 1836 + /- 273 pg / ml versus 9635 + /- 3437 pg / ml , P = 0.04 ) . - pubmed
A promising approach to the gene therapy of PD is intrastriatal expression of dopamine - synthesizing enzymes [ tyrosine hydroxylase ( TH ) and aromatic L - amino acid decarboxylase ( AADC ) ] . - pubmed
Combination of dopamine - supplement gene therapy and GDNF gene therapy would be a logical approach to the treatment of PD . - pubmed
All ten of the possible five - carbon acyclic primary beta- , gamma- , and delta - amino alkanoic acids ( amino position isomers of the valines ) have been positively identified in hot - water extracts of the Murchison meteorite using combined gas chromatography - mass spectrometry ( GC - MS ) and ion exchange chromatography . - pubmed
Fifteen months after deworming with albendazole and praziquantel the prevalence of anaemia was reduced by a quarter and that of moderate - to - severe anaemia ( haemoglobin & lt;90 g / l ) was reduced by nearly a half . - pubmed
The maintenance of oxygen supply to the tissues during anaemia involves complex cardiovascular adaptations , including an increase in cardiac output , reduced peripheral resistance and increased oxygen extraction from haemoglobin ( Hb ) . - pubmed
Many potential reasons for erythropoietin - induced hypertension in uraemic patients have been postulated , including increased blood viscosity as haematocrit rises , a reversal of hypoxic vasodilatation , increased blood volume that is not compensated by haemodialysis , ultrafiltration and impaired nitric oxide synthesis , preventing vascular relaxation in response to increased blood viscosity . - pubmed
Similarity with wild - type receptors has also been demonstrated by the differential insertion of insulin - bound and free HIR - MAED complexes into artificial bilayer membranes of Triton X-114 . - pubmed
[ Stress - rest myocardial tomoscintigraphy with 99mTc - tetrofosmin in the diagnosis of ischemic disease ] . - pubmed
injections of 99mTc tetrofosmin , one at peak exercises ( 370 MBq ) and the other ( 740 MBq ) at rest 3 h after exercise ( images 15 - 30 min after injections for both studies ) . - pubmed
The neointimal area at 28 days was not diminished , however , by NO eluded from either stents of thin or thick barriers ( control bare stent - 0.66 mm2 , control PU stent - 0.68 mm2 , SNP - PU thin coating stent - 0.78 mm2 , SNP - PU thick coating stent - 0.85 mm2 ; all p = NS ) . - pubmed
The clinical data with ranolazine focuses on its use in chronic stable angina , where it has been shown to increase exercise tolerance and decrease angina compared with placebo and in combination with beta - blockers and calcium - channel blockers . - pubmed
Asp177 , conserved in all PP(i ) PFKs , negates the binding of the alpha - phosphate group of ATP and likely contacts the essential Mg ( 2 + ) cation via a water molecule . - pubmed
Mesenteric / SAF ratio of IL-6 , TNF - alpha , corticosterone , and gene profiling showed decrease of inflammation after RYGB . - pubmed
The combination of indole-3 - acetic acid ( IAA ) and horseradish peroxidase ( HRP ) has recently been proposed as a novel cancer therapy . - pubmed
It is known that IAA produces free radicals when oxidized by HRP . - pubmed
Because oxidative stress could induce apoptosis , we measured the production of free radicals at varying concentrations of IAA and HRP . - pubmed
Our results show that IAA / HRP produces free radicals in a dose - dependent manner , which are suppressed by ascorbic acid or ( -)- epigallocatechin gallate ( EGCG ) . - pubmed
In addition , IAA / HRP was observed to activate p38 mitogen - activated protein ( MAP ) kinase and c - Jun N - terminal kinase ( JNK ) , which are almost completely blocked by antioxidants . - pubmed
We further investigated the IAA / HRP - mediated apoptotic pathways , and found that IAA / HRP activates caspase-8 and caspase-9 , leading to caspase-3 activation and poly ( ADP - ribose ) polymerase ( PARP ) cleavage . - pubmed
IH induced significant decreases in Ser-133 phosphorylated CREB ( pCREB ) without changes in total CREB , starting as early as 1 h IH , peaking at 6 h-3 days , and returning toward normoxic levels by 14 - 30 days . - pubmed
In contrast , the presence of 5 Chordin - like cysteine - rich domains in the CV-2 protein suggests that CV-2 belongs to a family of well - established inhibitors of BMP function that includes Chordin and Sog [ Development 127 ( 2000 ) 3947 ] . - pubmed
The LPS - induced inhibition of the neurogenic vasopressor response was counteracted by the cannabinoid CB ( 1 ) receptor antagonist SR 141716A ( 0.1 micromol x kg(-1 ) ) , but not by the CB ( 2 ) receptor antagonist SR 144528 ( 3 micromol x kg(-1 ) ) , the vanilloid VR1 receptor antagonist capsazepine ( 1 micromol x kg(-1 ) ) or the histamine H ( 3 ) receptor antagonist clobenpropit ( 0.1 micromol x kg(-1 ) ) . - pubmed
We conclude that in the initial phase of septic shock , the activation of presynaptic CB ( 1 ) receptors by endogenously formed cannabinoids contributes to the inhibition of the neurogenic vasopressor response . - pubmed
At various time points postrelease , matrix lysates were blotted for the proteins of interest , and the terminal deoxynucleotidyltransferase - mediated dUTP nick - end label assay was performed on both whole matrices and cytospin preparations . - pubmed
RESULTS : Terminal deoxynucleotidyltransferase - mediated dUTP nick - end label positivity in attached versus detached matrices ( at 24 h post detachment ) was 0.7 + /- 03 versus 5.3 + /- 1.7 % ( P & lt ; 0.05 , unpaired t test ) . - pubmed
Biotin - rich , optically clear nuclei express estrogen receptor - beta : tumors with morules may develop under the influence of estrogen and aberrant beta - catenin expression . - pubmed
We investigated a possible role of estrogen receptor ( ER )- beta in the occurrence of tumors that form morules with biotin - rich optically clear nuclei ( BROCN)(BROCN - family tumors ) and its possible relationship with aberrant nuclear / cytoplasmic ( N / C ) beta - catenin expression . - pubmed
Whether the estrogen - signaling pathway and the Wnt - signaling pathway have crosstalk , cooperating in the development of the BROCN - family tumors , awaits further study . - pubmed
Isoflavone synthase ( IFS ) catalyzes the key entry point step of isoflavone biosynthesis from the general phenylpropanoid pathway . - pubmed
The defense related hormone salicylic acid induced the expression of IFS1 : GUS in all cell types of the root . - pubmed
We estimated the relative risk ( RR ) for each lipid parameter , adjusted for C - reactive protein , homocysteine , body mass index , family history , hypertension , diabetes , postmenopausal hormone use , physical activity , alcohol intake , and blood draw parameters . - pubmed
The RRs associated with an increase of approximately 1 SD ( mg / dL ) were as follows : HDL cholesterol ( HDL - C ) ( RR=0.6 [ 0.5 to 0.8 ] , SD=17 ) , apolipoprotein B100 ( apoB100 ) ( RR=1.7 [ 1.4 to 2.1 ] , SD=32 ) , LDL cholesterol ( LDL - C ) ( RR=1.4 [ 1.1 to 1.7 ] , SD=36 ) , total cholesterol ( TC ) ( RR=1.4 [ 1.1 to 1.6 ] , SD=40 ) , and triglycerides ( RR=1.3 [ 1.0 to 1.5 ] , SD=80 ) . - pubmed
After simultaneous control for several lipid biomarkers , HDL - C was the primary contributor of the variation in multivariate models ( P=0.01 ) , followed by LDL - C ( P=0.01 ) , whereas triglycerides and apoB100 did not contribute further information . - pubmed
The hepatitis C virus ( HCV ) RNA - dependent RNA polymerase ( RdRp ) has several distinct biochemical activities , including initiation of RNA synthesis by a de novo mechanism , extension from a primed template , nontemplated nucleotide addition , and synthesis of a recombinant RNA product from two or more noncovalently linked templates ( template switch ) . - pubmed
While the prototype enzyme had a K(m ) for GTP of 3.5 microM , all of the mutations except one had K(m)s that were three- to sevenfold higher . - pubmed
Using biotin-4 - fluorescein , the detection limit of streptavidin lies in the same range as with Coomassie blue staining . - pubmed
Based on well - controlled clinical trials , vasodilators such as misoprostol , and histamine receptor ligands should be further investigated . - pubmed
Finally , given the evidence that ST is a form of sensory epilepsy , new antiepileptic drugs should be tested for potential efficacy as they are developed ; such drugs may include novel N - methyl - D - aspartate receptor antagonists , as well as cannabinoids . - pubmed
These foci colocalized with those of Rad51 and phospho - DNA - PKcs . - pubmed
Furthermore , gammaH2AX and phospho - DNA - PKcs foci were localized at expressed fragile sites on metaphase chromosomes . - pubmed
Intergastric treatment with NAC or SMC five times a week resulted in decreased numbers and areas of preneoplastic , glutathione S - transferase placental form ( GST - P ) positive foci of the liver in a dose - dependent manner . - pubmed
Moreover , cell proliferation was reduced in GST - P positive foci by NAC and SMC . - pubmed
Insulin - like growth factor I ( IGF - I ) and inducible nitric oxide synthase ( iNOS ) mRNA expressions were found downregulated in the liver by NAC . - pubmed
Programme attendance : CBT + Acamprosate group ( 66.1 % ) , CBT + Naltrexone group ( 79.7 % ) , and in the CBT + Combined group ( 83.1 % ) . - pubmed
RESEARCH METHODS AND PROCEDURES : Isolated retroperitoneal adipocytes were cultured for 0.5 to 48 hours without ( baseline ) or with ( 0.001 to 1 nM ) ghrelin alone or in combination with insulin ( 0.01 to 10 nM ) or dexamethasone ( 1 to 100 nM ) . - pubmed
Adipocytes were also incubated with ghrelin and inhibitors either of RNA ( actinomycin D ) or protein synthesis ( cycloheximide ) or with several concentrations ( 10 to 1000 nM ) of a specific ghrelin antagonist . - pubmed
RESULTS : Our data indicate that ghrelin directly enhanced adipocyte leptin release and ob mRNA expression , that the leptin - releasing activity of ghrelin was additive to the action of both insulin and dexamethasone and was abrogated by protein synthesis inhibitors , and that effects of ghrelin on adipocyte ob mRNA expression and release were blocked by coincubation with the specific growth hormone secretagogue receptor 1a antagonist . - pubmed
Endothelial response to stress from exogenous Zn2 + resembles that of NO - mediated nitrosative stress , and is protected by MT-1 overexpression . - pubmed
Furthermore , we found Zn ( 2 +)-exposed PAECs exhibited a significant reduction in mitochondrial membrane potential , loss of cardiolipin from the inner leaflet , caspase activation , and significant increases in TdT - mediated dUTP nick end labeling - positive cells . - pubmed
Furthermore , using an adenoviral construct for the overexpression of the Zn ( 2 +)-binding protein , metallothionein-1 ( MT-1 ) , we found either MT-1 overexpression or coincubation with a Zn ( 2 +)-selective chelator , N , N , N ' , N '- tetrakis ( 2 - pyridylmethyl ) ethylene - diamide , in PAECs significantly protected the mitochondria from both NO and Zn ( 2 +)-mediated disruption and induction of apoptosis and cell death . - pubmed
In every case , we analyzed the plasma levels of glucose , ferritin and other biochemical parameters . - pubmed
The increased mitotic activity induced by CPG and mGnRH was abolished by the addition of mGnRH antagonists and anti - mGnRH antibody , suggesting that the spermatogonial proliferation is regulated by GnRH - like peptide in CPG of the scallop . - pubmed
These findings suggest that the spermatogonial proliferation at phase II in the scallop may be under the neuroendocrine control by GnRH neuron in CPG . - pubmed
In this study , horseradish peroxidase ( HRP)-labelled monoclonal anti - LH can catalyse the luminol - hydrogen peroxide reaction . - pubmed
Within 14 min , free enzyme conjugate and immune complex could be separated in alkaline borate buffer by means of a high voltage ( 15 kV ) . - pubmed
One of the earliest events produced by this hormone is inhibition of the plasma membrane enzyme adenylyl cyclase ( AC ) , with the concomitant drop in intracellular cAMP levels and reinitiation of the cell cycle . - pubmed
Overexpression of xRic-8 , through mRNA injection , greatly inhibits progesterone induced oocyte maturation and endogenous xRic-8 mRNA depletion , through siRNA microinjection , induces spontaneous oocyte maturation . - pubmed
On the other hand , the expression of PvNADH - GOGAT - I , but not PvNADH - GOGAT - II , was inhibited with nitrogen compounds . - pubmed
In silico analyses of the NADH - GOGAT promoters revealed the presence of potential cis elements in them that could mediate differential tissue - specific , and sugar and amino acid responsive expression of these genes . - pubmed
Phenylglycine and phenylalanine derivatives as potent and selective HDAC1 inhibitors ( SHI-1 ) . - pubmed
An HTS screening campaign identified a series of low molecular weight phenols that showed excellent selectivity ( & gt;100 - fold ) for HDAC1 / HDAC2 over other Class I and Class II HDACs . - pubmed
Two - dimensional temperature fields are measured in lean and sooting flames by means of two - color laser - induced fluorescence ( LIF ) imaging that uses seeded NO . - pubmed
Group 3 received phosphate buffered saline solution ( PBS ) ICI and 2.5 x 10(11 ) vpu Ad beta - gal ICI , 10 days later . - pubmed
Total and phosphorylated Akt and endothelial nitric oxide were analyzed by Western blot . - pubmed
The VEGF trap eliminated the beneficial effects of bFGF on endothelium - dependent vasoreactivity and decreased Akt and nitric oxide phosphorylation . - pubmed
This article provides an overview of the prevalence , aetiology , and mechanisms of tissue injury arising as a result of post - stroke hyperglycaemia , as well as exploring the evidence from glucose - lowering treatment trials to date . - pubmed
Nitric oxide ( NO ) is a reactive molecule synthesized by NO synthases ( NOS ) . - pubmed
During the study the following were measured : estradiol ( E2 ) , progesterone ( P4 ) , follicle - stimulating hormone ( FSH ) , luteinizing hormone ( LH ) , and histopathological study of the uteri , tubes , and ovaries . - pubmed
In the present study , we show that truncated Delta7 - ERalpha attenuates WT - ERalpha - driven gene expression across a wide range of estrogen concentrations in cells that express functional ERalpha at base line or upon co - transfection of full - length ERalpha . - pubmed
METHODS AND RESULTS : When HCAECs were treated with recombinant cHSP60 , endothelial nitric oxide synthase ( eNOS ) mRNA and protein levels , enzyme activities , cellular NO levels , mRNA stability , and promoter activities were significantly decreased . - pubmed
Superoxide anion production was significantly increased due to the inhibition of mitochondrial membrane potential and catalase and superoxide dismutase ( SOD ) activities as well as activation of NADPH oxidase . - pubmed
Antioxidant seleno - l - methionine ( SeMet ) or SOD mimetic MnTBAP effectively blocked cHSP60 - induced eNOS downregulation . - pubmed
The parent drug and the potential metabolites were identified by two independent qualitative parameters , retention time ( tR ) and MS / MS spectrum . - pubmed
Plateau potentials and persistent activity were suppressed by the generic nonselective cation channel blockers FFA ( 100 muM ) and 2 - APB ( 100 muM ) , as well as by the TRPC channel blocker SKF-96365 ( 50 muM ) . - pubmed
However , plateau potentials were not affected by the TRPV channel blocker ruthenium red ( 40 muM ) . - pubmed
The TRPC3 / 6 / 7 activator OAG did not induce or enhance persistent firing evoked by carbachol . - pubmed
Voltage clamp recordings revealed a carbachol - activated , nonselective cationic current with a heteromeric TRPC - like phenotype . - pubmed
Participants were randomized either to treatment as usual ( TAU ) or to TAU plus CBT - GSH . - pubmed
RESULTS : Compared to those receiving TAU only , those who received TAU plus CBT - GSH experienced 25.2 more binge - free days and had lower total societal costs of $ 427 over 12 months following the intervention ( incremental CEA ratio of -$20.23 per binge - free day or -$26,847 per QALY ) . - pubmed
Lower costs in the TAU plus CBT - GSH group were due to reduced use of TAU services in that group , resulting in lower net costs for the TAU plus CBT group despite the additional cost of CBT - GSH . - pubmed
CONCLUSIONS : Findings support CBT - GSH dissemination for recurrent binge - eating treatment . - pubmed
This potentiating effect of H ( 2 ) O ( 2 ) was blocked by the pretreatment with either 10,000 - unit / mL catalase or 300 - microM N - acetyl - cysteine . - pubmed
The potentiating effect of H ( 2 ) O ( 2 ) was occluded by an adenylate cyclase activator , forskolin , and was blocked by a protein kinase A inhibitor , N -( 2 -[ p - bromocinnamylamino ] ethyl )- 5 - isoquinolinesulfonamide hydrochloride . - pubmed
This study indicates that oxidative stress may potentiate presynaptic GABA release through the mechanism of cAMP - dependent protein kinase A ( PKA)-dependent pathways , which may result in the inhibition of the cerebral cortex neuronal activity . - pubmed
Selective knockdown of ceramide synthases reveals complex interregulation of sphingolipid metabolism . - pubmed
However , the contribution of each CerS to steady - state levels of specific Cer species has not been evaluated . - pubmed
Additionally , each CerS knockdown produced unique effects on the levels of multiple sphingolipid species . - pubmed
For example , downregulation of CerS2 decreased very long - chain Cer but increased levels of CerS4 , CerS5 , and CerS6 expression and upregulated long - chain and medium - long - chain sphingolipids . - pubmed
Conversely , CerS6 knockdown decreased C16:0 - Cer but increased CerS5 expression and caused non - C16:0 sphingolipids to be upregulated . - pubmed
Knockdown of individual CerS failed to decrease total sphingolipids or upregulate sphingoid bases . - pubmed
Treatment with siRNAs targeting combined CerS , CerS2 , CerS5 , and CerS6 , did not change overall Cer or sphingomyelin mass but caused upregulation of dihydroceramide and hexosyl - ceramide and promoted endoplasmic reticulum stress . - pubmed
These data suggest that sphingolipid metabolism is robustly regulated by both redundancy in CerS - mediated Cer synthesis and counter - regulation of CerS expression . - pubmed
SGK3 is an estrogen - inducible kinase promoting estrogen - mediated survival of breast cancer cells . - pubmed
Here we report that SGK3 is an estrogen receptor ( ER ) transcriptional target and promotes estrogen - mediated cell survival of ER - positive breast cancer cells . - pubmed
In ER - positive breast cancer cells , SGK3 expression was found to be induced by 17beta - estradiol ( E(2 ) ) in a dose- and time - dependent manner , and the induction of SGK3 mRNA by E(2 ) is independent of newly synthesized proteins . - pubmed
Together , our study defines the molecular mechanism of regulation of SGK3 by estrogen / ER and provides a new link between the PI3 K pathway and ER signaling as well as a new estrogen - mediated cell survival mechanism mediated by SGK3 in breast cancer cells . - pubmed
The DIF derivatives differentially affected cell differentiation and chemotaxis , suggesting the possible existence of at least three receptors for DIFs : one receptor responsible for stalk cell induction , and two receptors responsible for chemotaxis modulation . - pubmed
Furthermore , our results indicate that DIF derivatives can be utilized to analyze the DIF - signaling pathways . - pubmed
RESULTS : High glucose increased MMP2 and decreased connexin 43 in the mitochondria of retinal endothelial cells . - pubmed
Blood pressure - dependent and independent effects of amlodipine and angiotensin receptor blockers on the risk of stroke . - pubmed
Both transport purine and pyrimidine nucleosides and are distinguished functionally by a difference in sensitivity to inhibition by nanomolar concentrations of nitrobenzylmercaptopurine ribonucleoside ( NBMPR ) , hENT1 being NBMPR - sensitive . - pubmed
The same study also revealed lower , but significant , transport of hypoxanthine by h / rENT1 . - pubmed
Fluxes of hypoxanthine , thymine , and adenine by hENT1 were saturable and inhibited by NBMPR . - pubmed
Nucleobase transport activity was absent from an engineered cysteine - less version hENT1 ( hENT1C- ) in which all 10 endogenous cysteine residues were mutated to serine . - pubmed
Site - directed mutagenesis identified Cys-414 in transmembrane helix 10 of hENT1 as the residue conferring nucleobase transport activity to the wild - type transporter . - pubmed
Substitution of a single amino acid residue in the aromatic / arginine selectivity filter alters the transport profiles of tonoplast aquaporin homologs . - pubmed
X - ray crystallography of aquaporins indicates that four amino acids constitute an aromatic / arginine ( ar / R ) pore constriction known as the selectivity filter . - pubmed
TgTIP1;1 and TgTIP1;2 have a novel isoleucine in loop E ( LE2 position ) of the ar / R filter ; the residue at LE2 is a valine in all group I TIPs from model plants . - pubmed
TgTIP1;1- and TgTIP1;2 - mediated H ( 2 ) O ( 2 ) conductance was demonstrated further by a fluorescence assay . - pubmed
In contrast , mutants that mimicked group II TIPs showed no evidence of urea or H ( 2 ) O ( 2 ) conductance . - pubmed
These results suggest that the amino acid residue at LE2 position is critical for the transport selectivity of the TIP homologs and group I TIPs might have a broader spectrum of substrate selectivity than group II TIPs . - pubmed
METHODS : As aspirin is released from prodrugs following esterase hydrolysis we studied the expression and activity of butyrylcholineterase ( BuChE ) and carboxyesterase ( CE ) in plasma and platelets . - pubmed
Finally , the relative contribution of nitric oxide ( NO ) bioactivity to nitrate - containing prodrugs of aspirin - induced inhibition of aggregation was determined using 1H -[ 1,2,4 ] oxadiazolo [ 4,3 - a ] quinoxalin-1 - one ( ODQ , ) a selective inhibitor of the soluble guanylyl cyclase . - pubmed
KEY FINDINGS : ST0702 , 2 , a nicotinic acid - aspirin codrug was equipotent with aspirin with respect to inhibition of collagen - induced platelet aggregation . - pubmed
The platelet inhibitory effects of aspirin prodrugs were time - dependent as the maximal inhibitory effects against collagen - induced aggregation were achieved by aspirin at 2 min , 1 at 5 min and ST0702 at 15 min . - pubmed
The aspirin prodrugs were significantly less potent in WP than in PRP and the reverse was true of aspirin . - pubmed
In the presence of complete BuChE inhibition in PRP , there was almost complete loss of aspirin prodrug , but not aspirin anti - aggregatory activity . - pubmed
Interestingly , CE activity was observed in WP and platelet lysate with pNPA substrate . - pubmed
Accordingly , 1 and ST0702 retained 50 % and 100 % anti - aggregatory activity at maximal concentrations in WP , which was attenuated in the presence of esterase inhibitor phenylmethylsulphonyl fluoride . - pubmed
CONCLUSIONS : The inhibitory effect of aspirin prodrugs in PRP is due to prodrug activation by BuChE. - pubmed
DC were matured for 24 h with TNF - alpha and prostaglandin E ( 2 ) ( PGE ( 2 ) ) . - pubmed
The static respiration tests led to 2 - 2.5 times lower oxygen - related indices compared to those calculated in dynamic respiration tests ( DRT ) ; the 7 - day CO ( 2 ) cumulative generations were , however , almost similar for both types of tests . - pubmed
AIM : To determine the prevalence and to identify the risk factors of chloroquine maculopathy ( CM ) , and to evaluate the association of plasma chloroquine ( CQ ) and desethylchloroquine ( DCQ ) levels and CM . - pubmed
Synergistic enhancement of cancer therapy using a combination of heat shock protein targeted HPMA copolymer - drug conjugates and gold nanorod induced hyperthermia . - pubmed
In this study , tumor hyperthermia was utilized as a means to increase the active delivery of heat shock protein ( HSP ) targeted N -( 2 - hydroxypropyl ) methacrylamide ( HPMA ) copolymer - drug conjugates . - pubmed
Conjugates bearing the anticancer agents aminohexylgeldanamycin ( AHGDM ) , docetaxel ( DOC ) , or cisplatin and the GRP78 targeting peptide WDLAWMFRLPVG were synthesized and characterized . - pubmed
HSP targeted AHGDM and DOC conjugates demonstrated active binding comparable to native targeting peptide . - pubmed
Based on these results , HSP targeted DOC conjugates were selected for in vivo evaluation . - pubmed
( Photochem Photobiol 10:309 - 329 , 1969 ) designed and employed an elegant and highly sensitive electrochemical technique capable of measuring O2 evolved by photosystem II ( PSII ) in response to trains of single turn - over light flashes . - pubmed
The measurement and analysis of flash - induced oxygen evolution patterns ( FIOPs ) has since proven to be a powerful method for probing the turnover efficiency of PSII . - pubmed
Cell cycle analysis , methyl - thiazol - diphenyltetrazolium assay , alkaline phosphatase activity , mineralization behavior , and gene expression of odontoblast phenotype SCAP cultured with or without CM from PAFCs were evaluated . - pubmed
Analysis of the assembled contigs via two different methods , produced 36 new pre - pro - peptides , coding for 61 neuropeptides or peptide hormones belonging to the following families : Allatostatins ( A , B et C ) , Bursicon ( alpha and beta ) , Crustacean Hyperglycemic Hormones ( CHH and MIH / VIHs ) , Crustacean Cardioactive Peptide ( CCAP ) , Corazonin , Diuretic Hormones ( DH ) , the Eclosion Hormone ( EH ) , Neuroparsin , Neuropeptide F ( NPF ) , small Neuropeptide F ( sNPF ) , Pigment Dispersing Hormone ( PDH ) , Red Pigment Concentrating Hormone ( RPCH ) and finally Tachykinin . - pubmed
We also analyzed the transcripts of three citrate channel proteins and found they were up - regulated by NaCl , CuSO4 , and AlCl3 but not by IM or DM . - pubmed
APPROACH AND RESULTS : Platelets displayed all 3 primary proteasome protease activities , which MG132 and bortezomib ( Velcade ) inhibited . - pubmed
The effects of proteasome inhibitors were not confined to a single receptor as MG132 suppressed thrombin - stimulated , ADP - stimulated , and lipopolysaccharide - stimulated microparticle shedding . - pubmed
Mass spectrometry revealed a single MG132 - sensitive tryptic cleavage after R1745 in an extended Filamin A loop , which would separate its actin - binding domain from its carboxy terminal glycoprotein Ibalpha - binding domain . - pubmed
These findings show that progesterone increases n-3 PUFA biosynthesis by up - regulating the mRNA expression of genes involved in this pathway , possibly via changes in the epigenetic regulation of FADS2 . - pubmed
Here , we analysed the lipids in inflammatory exudates using liquid chromatography - tandem mass spectrometry and identified a novel DHA metabolite , 14,20 - dihydroxy - DHA ( 14,20 - diHDHA ) and showed that it is biosynthesized by eosinophils through the 12 / 15 - lipoxygenase pathway . - pubmed
White " x - ray radiation from the Advanced Photon Source was used to initiate the chemical decomposition of C6F14 , which resulted in the in situ production of F2 as verified via Raman spectroscopy . - pubmed
Induction of H2AX phosphorylation in tumor cells by gossypol acetic acid is mediated by phosphatidylinositol 3 - kinase ( PI3 K ) family . - pubmed
Thus , we further examined the possible mechanisms of GAA to induce gammaH2AX in tumor cells . - pubmed
However , it has been less well described for icotinib , a similar orally small - molecule tyrosine kinase inhibitor ( TKI ) . - pubmed
Disulfide - bond scrambling promotes amorphous aggregates in lysozyme and bovine serum albumin . - pubmed
In this study , we report the effect of disulfide - reducing agent dithiothreitol ( DTT ) on hen egg white lysozyme ( lysozyme ) and bovine serum albumin ( BSA ) aggregation at pH 7.2 and 37 C. BSA and lysozyme proteins treated with disulfide - reducing agents form very distinct amorphous aggregates as observed by scanning electron microscope . - pubmed
BSA and lysozyme aggregates show unique but measurable differences in 8 - anilino-1 - naphthalenesulfonic acid ( ANS ) and 4,4 '- dianilino-1,1 '- binaphthyl-5,5 '- disulfonic acid ( bis - ANS ) fluorescence , suggesting a loose and flexible aggregate structure for lysozyme but a more compact aggregate structure for BSA . - pubmed
Similar amorphous aggregates were also generated using a nonthiol - based reducing agent , tris ( 2 - carboxyethyl ) phosphine ( TCEP ) , at pH 7.2 and 37 C. In summary , formation of distinct amorphous aggregates by disulfide - reduced BSA and lysozyme suggests an alternate pathway for protein aggregation that may be relevant to several proteins . - pubmed
Binding of different ligands like GalNAc , lactose , and galactose onto native and demetallized protein was studied by isothermal titration calorimetry as well as molecular simulation methods . - pubmed
Design , synthesis and biological evaluation of novel pyrazoline - containing derivatives as potential tubulin assembling inhibitors . - pubmed
Total synthesis of a biotinylated rocaglate : Selective targeting of the translation factors eIF4AI / II . - pubmed
Affinity purification revealed that biotinylated rocaglate ( 8) and methyl rocaglate ( 2 ) bind with high specificity to translation factors eIF4AI / II . - pubmed
RESULTS : Thyroid - stimulating hormone , vitamin B12 and insulin levels were significantly lower in the study group than controls ( p=0.001 , p=0.049 and p=0.033 , respectively ) . - pubmed
All had a circumscribed central maculopathy with borders that did not respect posterior pole horizontal arterioles ( typically extending beyond the major arcades ) and associated with polygonal pigment clumping . - pubmed
To target extended - spectrum beta - lactamase ( ESBL)-producing pathogens , novel beta - lactam antibiotics , such as ceftolozane / tazobactam , ceftazidime / avibactam , aztreonam / avibactam , imipenem / relebactam and S-649266 , are antimicrobial alternatives for cIAIs . - pubmed
The FA and AD values in the left posterior limb of internal capsule correlated negatively with the heroin - positive urinalysis rate within follow - up . - pubmed
These observations prompted the US FDA to revise statin labels to include a warning of an increased risk of incident diabetes mellitus as a result of increases in glycosylated hemoglobin ( HbA1c ) and fasting plasma glucose . - pubmed
RESULTS : Risk factors associated with MRSA SSIs identified by the Delphi method were : patients from long - term care facilities , recent hospitalization ( within the preceding 30 days ) , Charlson score & gt ; 5 points , chronic obstructive pulmonary disease and thoracic surgery , antibiotic therapy with beta - lactams ( especially cephalosporins and carbapenem ) and/or quinolones in the preceding 30 days , age 75 years or older , current duration of hospitalization & gt;16 days , and surgery with prothesis implantation . - pubmed
LY294002 induces differentiation and inhibits invasion of glioblastoma cells by targeting GSK-3beta and MMP . - pubmed
Taken together , these findings suggest differentiation - inducing and invasion - inhibitory effectiveness of LY294002 in glioblastomas , most likely involving inhibition of GSK-3beta and MMP respectively . - pubmed
Low - Density Lipoprotein Cholesterol , Non - High - Density Lipoprotein Cholesterol , Triglycerides , and Apolipoprotein B and Cardiovascular Risk in Patients With Manifest Arterial Disease . - pubmed
Low - density lipoprotein cholesterol ( LDL - C ) only partly represents the atherogenic lipid burden , and a growing body of evidence suggests that non - high - density lipoprotein cholesterol ( non - HDL - C ) , triglycerides , and apolipoprotein B ( apoB ) are more accurate in estimating lipid - related cardiovascular disease risk . - pubmed
Adjusted hazard ratios ( HRs ) of MACE per 1 SD higher were for LDL - C ( HR 1.15 , 95 % confidence interval [ CI ] 1.09 to 1.22 ) , for non - HDL - C ( HR 1.17 , 95 % CI 1.11 to 1.23 ) , for log(triglycerides ) ( HR 1.12 , 95 % CI 1.06 to 1.19 ) , and for apoB HR ( 1.12 , 95 % CI 0.99 to 1.28 ) . - pubmed
Transcription factors such as NST1 may regulate genes involved in phenylpropanoid pathway and affect development and lignification of the endocarp . - pubmed
Moreover , a 3 year - old Vitamin E / HPbetaCD - IC NF sample has shown very similar antioxidant efficiency when compared with freshly prepared Vitamin E / HPbetaCD - IC NF indicating that long - term stability was achieved for Vitamin E in the CD - IC fiber matrix . - pubmed
First simultaneous generation and utilization of both copper - triazole and - ketenimine intermediates in copper - catalyzed sulfonyl azide - alkyne cycloaddition reactions is achieved for the one - pot synthesis of triazolopyrimidines via a novel copper - catalyzed multicomponent cascade of sulfonyl azides , alkynes , and azirines . - pubmed
Through the important women who lived their lives fighting against this disease and its complications , we would like to pay tribute to all those women who still today , in an era of antibiotics and where syphilis is a treatable pathology , they can not be cured because they are both geographically and socially disadvantaged . - pubmed
To check the applicability of JADE in real cases , JADE was used for resolution and quantification of phenanthrene and anthracene in aromatic fraction of heavy fuel oil ( HFO ) analyzed by GCxGC - MS . - pubmed
In addition , LOD values ( mug / mL ) were respectively 1.36 and 1.24 for phenanthrene and 1.26 and 1.09 for anthracene using MCR - ALS and JADE which showed outperformance of JADE over MCR - ALS . - pubmed
A liquid chromatography - high resolution mass spectrometry ( LC - HRMS ) method was developed and validated for the determination of piperine ( PPR ) on dried blood spots ( DBS ) . - pubmed
Results showed that ETB receptor agonist , sarafotoxin 6c induced a negligible contraction in fresh artery segments , while ETA receptor agonist , ET-1 induced an obvious contraction . - pubmed
The U0126 ( MEK / ERK1 / 2 inhibitor ) and SB203580 ( p38 inhibitor ) significantly attenuated both organ cultured - induced and PM2.5 - induced up - regulation of ETB receptor . - pubmed
U0126 also suppressed organ culture - increased and PM2.5 - increased expressions of ETA receptor . - pubmed
SB203580 only suppressed PM2.5 - induced enhanced expressions of ETA receptor In conclusion , airborne PM2.5 up - regulates ETB and ETA receptors of mesenteric artery via p38 MAPK and MEK / ERK1 / 2 MAPK pathways . - pubmed
Thyroid H2O2 generation was increased in DM , which was reversed by MSC treatment . - pubmed
A strong peak between 2 and 4 keV showed the greatest ratio of the elemental silver signals , due to surface plasmon resonance ( SPR ) . - pubmed
Immunofluorescence , Polscope equipment and quantitative reverse transcription polymerase chain reaction ( RT - qPCR ) were used to analyze damage , including mitochondrial distribution , reactive oxygen species ( ROS ) levels , spindle morphology , and gene expression levels ( Bcl-2 , BAX , and MnSOD ) . - pubmed
RT - qPCR showed that the expression of Bcl-2 was lower in the control group compared with the GSH - OEt group ; BAX and MnSoD expression levels were higher in the control group than in the GSH - OEt group ( p & lt ; 0.05 ) . - pubmed
I - X ) ; 2 ) LC - accurate MS / MS and quantitative 1H NMR spectroscopy conducted in parallel to acquire the qualitative and quantitative data of each fraction ; 3 ) Robust identification and quantification of components by use of LC coupled to multiple reaction monitoring . - pubmed
Using beta - CD as the particle core , polyethylene glycol ( PEG ) chains were conjugated to the primary face via non - cleavable bonds and amphiphilic polymers incorporating hydrophobic and cationic monomers were grafted to the secondary face via acid - labile linkages . - pubmed
We evaluated the efficacy of EPPT1 peptides presented on the free tips of the PEG brush to function as a targeting ligand against underglycosylated MUC1 ( uMUC1 ) receptors overexpressed on the surface of metastatic breast cancer cells . - pubmed
Overexpression of RCK1 significantly improved glucose and xylose fermentation under acetic acid stress conditions . - pubmed
Specifically , the RCK1 - overexpressing strain exhibited a two - fold higher specific ethanol productivity than the control strain in glucose fermentation under the presence of acetic acid . - pubmed
cerevisiae overexpressing RCK1 showed 40 % lower intracellular reactive oxygen species ( ROS ) levels as compared to the parental strain when the strains were exposed to acetic acid , suggesting that RCK1 overexpression might play a role in reducing the oxidative stress caused by acetic acid . - pubmed
A Polyamidoamine Dendrimer - Based Electrochemical Immunosensor for Label - Free Determination of Epithelial Cell Adhesion Molecule- Expressing Cancer Cells . - pubmed
The amino - terminated polyamidoamine dendrimer ( G6 PAMAM ) was first covalently attached to the 3 - mercaptopropionic acid ( MPA)-functionalized gold electrode to obtain a thin film , and then completely carboxylated by succinic anhydride ( SA ) . - pubmed
Next , the Anti - EpCAM was covalently bound with the G6 PAMAM to obtain a stable recognition layer . - pubmed
Notably , endothelial vasodilatation of mesenteric arteries was impaired in SHR adolescent offspring exposed to prenatal hypoxia , vascular responses to acetylcholine ( ACh ) and sodium nitroprusside ( SNP ) were reduced , as well as plasma nitric oxide levels and expression of endothelial nitric oxide synthase ( eNOS ) in vessels were decreased . - pubmed
Moreover , mesenteric arteries in SHR offspring following prenatal hypoxia showed enhanced constriction responses to phenylephrine ( PE ) , associated with up - regulated activities of L - type calcium channel ( Ca2 + -dependent ) , RhoA / Rock pathway signaling ( Ca2 + -sensitization ) , and intracellular Ca2 + flow . - pubmed
Furthermore , several proteins related to calcium signaling pathway and pathogen related proteins were up - regulated , and we also found expression levels of EDS1 , ICS1 , and PR1 involved in SA pathway up - regulated after DNPE6 ( 4 ) treatment . - pubmed
For Mcl-1 , a benzodioxane / benzoxazine series was optimized to a K d of 40 muM before a thienopyrimidine hit series was identified which subsequently led to the lead series from which the clinical candidate S 64315 ( MIK 665 ) was identified . - pubmed
For Bcl-2 , the fragment - derived series were difficult to progress , and a compound derived from a published tetrahydroquinone compound was taken forward as the hit from which the clinical candidate ( S 55746 ) was obtained . - pubmed
Oral glucose tolerance tests showed glucose intolerance and decreased insulin secretion in response to glucose loading in affected males , features which were exacerbated with age . - pubmed
Serum alanine / aspartate aminotransferases ( ALT / AST ) , liver glutathione ( GSH ) depletion , as well as the inflammatory cytokines , such as tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1beta ( IL-1beta ) , inducible nitric oxide synthase ( iNOS ) , and cyclooxygenase-2 ( COX-2 ) , were analyzed to indicate the underlying protective effects of Rb1 against APAP - induced hepatotoxicity with significant inflammatory responses . - pubmed
Importantly , Rb1 mitigated the changes in the phosphorylation of MAPK and PI3K / Akt , as well as its downstream factor NF - kappaB. - pubmed
In conclusion , experimental data clearly demonstrated that Rb1 exhibited a remarkable liver protective effect against APAP - induced ALI , partly through regulating MAPK and PI3K / Akt signaling pathways - mediated inflammatory responses . - pubmed
Therefore , our research aims to provide insights into the microstructure of K - A - S - H particles synthesized with accurate stoichiometric control by the hydrothermal method through thermogravimetric analysis ( TG ) , Fourier transform infrared spectroscopy ( FTIR ) , nuclear magnetic resonance ( NMR ) , scanning electron microscopy ( SEM ) , transmission electron microscopy ( TEM ) and BET surface area . - pubmed
The TG results indicate that the crystalline phase contains more non - evaporated water or zeolite water for structural rearrangement . - pubmed
Importantly , TRP channel TR structures have revealed sites and mechanisms of action of numerous synthetic and natural compounds , as well as those for endogenous ligands such as lipids , Ca2 + , and calmodulin . - pubmed
By contrast , under conditions of low cortisol change , individuals low in ERA outperformed those high in ERA . - pubmed
Canagliflozin ( CANA ) , a sodium - glucose cotransporter 2 ( SGLT2 ) inhibitor , affects various metabolisms . - pubmed
For instance , CANA significantly downregulated ATP synthase F1 subunit alpha , a mitochondrial electron transport system protein ( CON 297.28 + -20.63 vs. CANA 251.83 + -22.83 fmol/10 mug protein ; P = 0.0183 ) . - pubmed
Moreover , CANA significantly downregulated nucleoside diphosphate kinase 1 ( CON 110.30 + -11.37 vs. CANA 89.14 + -8.39 fmol/10 mug protein ; P = 0.0172 ) . - pubmed
Melatonin inhibits the endoplasmic reticulum stress - induced , C / EBP homologous protein - mediated pathway in acute pancreatitis . - pubmed
However , the mechanisms of the anti - inflammatory effects of melatonin ' are unknown , particularly the relationship between melatonin and ER stress . - pubmed
Moreover , cells treated with melatonin exhibited attenuated inflammation , decreased expression levels of ER stress - associated proteins and inhibition of apoptosis . - pubmed
Results : A meta - analysis of the data extracted from three randomized control trials revealed that treatment with exenatide yielded significant improvements in scores on the Unified Parkinson ' s Disease Rating Scale Part I ( UPDRS - I ) ( -0.438 , 95 % CI , -0.828 to -0.048 , p = 0.028 ) , UPDRS Part IV ( UPDRS - IV ) ( -0.421 , 95 % CI , -0.811 to -0.032 , p = 0.034 ) and the Mattis Dementia Rating Scale ( MDRS ) ( -0.595 , 95 % CI , -1.038 to -0.151 , p = 0.009 ) . - pubmed
More important , trelagliptin improved liver function by reducing alanine transaminase , aspartate transaminase , lactate dehydrogenase , and total bile acid . - pubmed
Mechanistically , we found that the administration of trelagliptin reduced the activity of hepatic nuclear factor - kappaB but increased the activity of AMP - activated protein kinase . - pubmed
We hypothesized that repeated short - term exposures of ammonia - oxidizing communities to free ammonia ( FA ) and free nitrous acid ( FNA ) would stabilize nitritation by selecting against nitrite - oxidizing bacteria ( NOB ) . - pubmed
A single stressor SBR ( SS / SBR ) using pH set - point control produced HNO3 , while a dual stressor SBR ( DS / SBR ) using timed alkalinity addition ( TAA ) produced HNO2 ( ammonium removal efficiency of 97 + - 2 % ; nitrite accumulation ratio of 98 + - 1 % ) . - pubmed
The diagnosis was stage IV pancreatic cancer(unresectable ) , and the patient underwent chemotherapy with GEM plus nab - PTX . - pubmed
This report examines the prevalence of SHS exposure among nonsmoking U.S. adults in 2015 - 2018 based on blood levels of cotinine , a metabolite of nicotine . - pubmed
In contrast , by adding DBD after PHA , RNA labelling was much more inhibited than DNA . - pubmed
When cyanocobalamin-57 Co was mixed with hog intrinsic factor in water , excretion increased to 15.6 % + /- 1.6 % . - pubmed
When cyanocobalamin-57 Co and intrinsic factor were given in two separate capsules , which is now a frequent practice , excretion was 9.5 % + /- 2.2 % . - pubmed
When cyanocobalamin-57 Co and intrinsic factor are given in separate capsules , binding of radioactive vitamin to intrinsic factor may be incomplete due to inadequate mixing in the stomach or to binding of intrinsic factor to blocking antibody in the gastric juice . - pubmed
The classic technique in which intrinsic factor and cyanocobalamin-57 Co are mixed in water before administration should be used . - pubmed
Recently , a female hormone , progesterone , has been found to inhibit the expression of alpha - fetoprotein in hepatoma cells . - pubmed
These results together with our hypothesis that the expression of alpha - fetoprotein is regulated by glucocorticoid receptor complex in proliferating hepatocellular carcinoma cells lead to the conclusion that steroid hormones and/or their antagonists may interfere with the function of glucocorticoid receptors in tumors , consequently regulate tumor growth . - pubmed
SslI activity was maximum at pH 8.8 , 0 to 50 mM NaCl , 2 to 8 mM Mg2 + , and 42 degrees C. Activity against phage DNA in vitro was demonstrated . - pubmed
BMP is the only molecule discovered thus far that is capable of inducing endochondral ossification in vivo . - pubmed
Although chronic ethanol treatment did not alter 2 - CADO- and NaF - stimulated adenylate cyclase activities in a cell - free preparation , 2 - CADO- , forskolin , and NaF - stimulated adenylate cyclase activities were reduced when assays were carried out in saponin - treated cells . - pubmed
Chronic ethanol exposure also increased the ability of in vitro ethanol to stimulate adenylate cyclase activity and cyclic AMP content . - pubmed
The increased sensitivity of adenylate cyclase to in vitro ethanol also was not observed when chronic ethanol treatment was carried out with media containing delipidated serum . - pubmed
Synergy between zinc and phorbol ester in translocation of protein kinase C to cytoskeleton . - pubmed
Treatment of cells for 40 min with a combination of zinc ( 2 - 50 microM ) , zinc ionophore pyrithione and unlabelled phorbol dibutyrate ( 200 nM ) caused up to a ten - fold increase in cytoskeletal protein kinase C and a corresponding decrease in other cellular compartments . - pubmed
We propose that zinc , like calcium , regulates the distribution of PKC in cells . - pubmed
However , unlike calcium which controls the binding of PKC to the lipid component on cell membranes , zinc controls the distribution of PKC to membrane cytoskeleton , possibly actin . - pubmed
[ The effect of corticosteroid on lymphokine - activated killer ( LAK ) activity and the change of membrane antigen phenotype on PBMC after IL2 incubation with reference to the evaluation in vitro ] . - pubmed
Moreover , the administration of corticosteroid may reduce the toxicity of IL2 . - pubmed
In this context , the effect of corticosteroid on LAK activity and the change of membrane antigen phenotype on PBMC ( peripheral blood mononuclear cells ) was studied in vitro by using methylprednisolone ( M - PSL ) . - pubmed
And the proliferation of PBMC after IL2 incubation , which was measured by incorporation of [ 3H ] TdR , was reduced dose - dependently by M - PSL . - pubmed
These results suggest that the inhibitory effect of M - PSL on LAK activation was caused not by preventing a triggering process of activation of precursor cells , but by a possible inhibition of proliferation , though other effects of corticosteroid remain to be elucidated . - pubmed
Like N2O reductase , NosA contained copper and was only made anaerobically . - pubmed
In contrast to N2O reductase , its synthesis was repressed by exogenous copper ( but not by Mn , Co , Ni , Zn , or Fe ) . - pubmed
To study whether or not atrial natriuretic peptide ( ANP ) is physiologically involved in body fluid volume regulation , we examined the relationship between plasma ANP level and renal function during NaCl loading and ANP infusion . - pubmed
The plasma ANP level increased by 60 % ( p less than 0.01 ) on the high NaCl diet . - pubmed
Although the plasma ANP level was higher in HTs than in NTs , the changes in plasma ANP due to NaCl loading were similar between the two groups . - pubmed
Furthermore , increases in urinary Na excretion due to ANP infusion at 25 ng / kg / min were greater for the high NaCl diet than for the low NaCl diet ( p less than 0.02 ) . - pubmed
In study II , graded doses of ANP were infusion into 16 other HTs and Nts on an 8 g / day NaCl diet . - pubmed
This rise in plasma ANP was comparable to that induced by high NaCl intake . - pubmed
Thus , a slight increase in plasma ANP induced by dietary Na loading seems to augment renal Na excretion , suggesting that ANP may play a physiological role in body fluid volume regulation . - pubmed
Densitometric lecithin / sphingomyelin ratios ( LSR ) and the presence of phosphatidylglycerol ( PG ) were determined for 735 consecutively received amniotic fluids . - pubmed
When the sample is introduced into the bioreactor , urea is first hydrolysed by urease to produce ammonia , which is then converted into L - glutamate by L - glutamate dehydrogenase . - pubmed
L - Glutamate is finally oxidized by L - glutamate oxidase to produce hydrogen peroxide , which is quantified by measuring chemiluminescence emitted upon admixing with luminol and potassium ferricyanide . - pubmed
Randomly selected patrons leaving taverns and bars were asked to participate in a study , and individuals agreed to answer a consumption questionnaire and have their blood alcohol level ( BAL ) measured . - pubmed
Acetone treatment did not alter the phospholipid and cholesterol content of the microsomes , whereas sodium phenobarbital ( positive control ) caused a significant decrease in cholesterol content / mg microsomal protein . - pubmed
Proceedings : Effect of intravenous infusion of oestradiol-17 beta , with and without prior progesterone treatment , on the plasma luteinizing hormone and follicle - stimulating hormone concentrations in anoestrous ewes . - pubmed
Analyses of such unusual microtubule arrays in taxol - treated cultures may provide valuable insights into tubulin - related systems in neurons , as well as in other cells . - pubmed
The deoxythymidine kinase ( dTK ) activity of a 5 - methoxymethyldeoxyuridine - resistant mutant ( MMdU(r)-20 ) of herpes simplex virus type 1 was compared with that of the parental wild - type ( WT ) virus . - pubmed
Further , it was inhibited to a lesser degree than the WT dTK by the nucleoside analogs MMdU and arabinosylthymine ( araT ) , which suggests that one of the effects of the mutation was a selective alteration in substrate recognition by the dTK . - pubmed
The loss of ability to inhibit the mutant dTK by E -( 2 )- 5 - bromovinyldeoxyuridine was not as great as that seen with araT and MMdU. - pubmed
[ 2 -( 14 ) C ] araT was used to explore further the resistance to araT. - pubmed
These findings suggest that a mutation in the dTK polypeptide has affected recognition not only of nucleoside substrates but of the nucleotide substrate dTMP as well , which agrees with the suggestion of Chen et al . - pubmed
Light density marrow cells ( LMDCs ) were preincubated in Biorich ( chemically defined serum - free complete medium ) with G + GM - CSF or IL-3 prior to semi - solid culture with Ara - C ( 10(-4 ) M-10(-12 ) M ) . - pubmed
Data from 26 normal marrows studied clonogenically , demonstrated a 60 % reduction in CFU - GM with 10(-4 ) M Ara - C. Preincubation in Biorich medium with the addition of G + GM - CSF or IL-3 caused a significant enhancement of sensitivity to Ara - C across the dose curve . - pubmed
Biorich medium alone had an apparent protective effect resulting in amelioration of the cytotoxic effect of Ara - C. Cell cycle analysis of eight subjects showed significant recruitment into S - phase following pre - treatment with cytokines . - pubmed
In summary , it would appear that cytokines can enhance the sensitivity of normal CFU - GM to Ara - C in vitro possibly due to an increase in S - phase activity . - pubmed
( + /-)- 1 -( Cyclohexyloxycarbonyloxy ) ethyl 2 - ethoxy-1 -[[ 2 '-( 1H - tetrazol-5 - yl ) biphenyl-4 - yl ] methyl ]- 1H - benzimidazole-7 - carboxylate ( TCV-116 , Candesartan ) and its active metabolite 2 - ethoxy-1 -[[ 2 '-( 1H - tetrazol-5 - yl ) biphenyl-4 - yl ] methyl ]- 1H - benzimidazole-7 - carboxylic acid ( CV-11974 ) are specific nonpeptide angiotensin AT1 receptor antagonists . - pubmed
AIF4- inhibits influx of Ca2 + into vascular smooth muscle cells . - pubmed
The cytoplasmic domain of Ig beta is sufficient to induce these early antigen - independent events by a mechanism that requires conserved tyrosine residues in this protein . - pubmed
Immunocompetition studies showed that import of precursors to Rieske FeS protein , ATPase su9 - DHFR , and the adenine nucleotide transporter was inhibited in the presence of antibody to MOM42 . - pubmed
The neurotensin antagonist [ D - Trp11 ]- neurotensin ( 3 pmol ) was previously found to enhance morphine , but not beta - endorphin antinociception from the periaqueductal gray ( PAG ) when injected into the RMg . - pubmed
A similar enhancement of morphine , but not beta - endorphin antinociception from the PAG was observed in the current study by [ D - Trp11 ]- neurotensin injections into the bilateral LPGi , bilateral Gi , or medial Gi . - pubmed
These data suggest that neurotensinergic projections from the PAG function in an antianalgesic manner throughout the RVM during morphine , but not beta - endorphin antinociception . - pubmed
Hypotonic stress ( HTS ) activates a Cl- current and releases intracellular Ca2 + in vascular endothelial cells , but there is no co - activation of K (+)- channels . - pubmed
Affinity purification of the 1 % Triton X-100 soluble stromal membrane fraction , on anti - ubiquitin - sepharose revealed a ubiquitinated 55 kD subunit . - pubmed
Using potassium hydroxide ( KOH ) and collagenase , the extracellular materials were successfully removed to expose the surface of muscle fibers . - pubmed
The other tested compounds : lysophospholipids , platelet - activating factor ( PAF ) and GM1 - ganglioside were without effect on A1 - AR expression . - pubmed
The inhibition with unsaturated FA was non - competitive and partially reversed by albumin . - pubmed
The A1 - AR agonist binding inhibition evoked by unsaturated FA in many respects is similar to that observed previously following ischaemia and may be contributory to the increased excitability of post - ischaemic brain . - pubmed
In order to investigate the relation between the conformation and the ability to modulate dopamine receptors , conformational free - energy calculations using an empirical potential ( ECEPP/3 ) and hydration shell model were carried out on the tripeptide + H2 - Pro - Leu - Gly - NH2 ( PLG ) and its analogues in which the Leu residue is replaced with Phe , Ahx ( L-2 - aminohexanoic acid ) , Ile , Abu and Ala residues in the unhydrated and hydrated states . - pubmed
PLG and two tripeptides possessing Phe and Ahx residues show dopamine receptor modulating activity , while the other tripeptides do not . - pubmed
Irrespective of activities , PLG and its analogues are found to have the high probabilities to form beta - bends of types II and I in the unhydrated state . - pubmed
A key player in the lambda DNA packaging process is the phage - encoded enzyme terminase , which is involved in ( i ) recognition of the concatemeric lambda DNA ; ( ii ) initiation of packaging , which includes the introduction of staggered nicks at cosN to generate the cohesive ends of virion DNA and the binding of the prohead ; ( iii ) DNA packaging , possibly including the ATP - driven DNA translocation ; and ( iv ) following translocation , the cutting of the terminal cosN to complete DNA packaging . - pubmed
The mechanism by which CPT induces cytotoxicity is the topic of continued research , but appears to be mediated by the stabilisation of transient " cleavable " topoisomerase I - DNA complexes . - pubmed
Furthermore , endothelial cell adhesion and spreading on type IV collagen stimulates the secondary signaling events of tyrosine phosphorylation of focal adhesion kinase ( pp125FAK ) and autophosphorylation of pp125FAK . - pubmed
CAI treatment of the endothelial cells inhibited cell spreading , and both the induction of pp125FAK phosphorylation and the phosphorylation of exogenous substrates by pp125FAK kinase . - pubmed
A model of the BR96 antibody variable regions is compared to two X - ray structures of a BR96 - carbohydrate complex , independently determined after the model was built and analyzed . - pubmed
Because ADR resistance in these cells is associated with the expression of high levels of P - glycoprotein ( Pgp ) , we evaluated the effect of anti - estrogens on Pgp expression and activity . - pubmed
Both ICI 182,780 and TAM failed to modulate Pgp expression as assessed by flow cytometry and Western - blot analysis , performed using the monoclonal antibodies MM4.17 and C 219 , which are specific for an external or an internal determinant respectively . - pubmed
Pgp activity was investigated by flow cytometry measuring the extrusion of ADR and the cationic dye Rhodamine 123 ( Rh 123 ) . - pubmed
Considerable physiological and anatomical evidence indicates that circulating angiotensin II ( AngII ) , plays important roles in the long - term regulation of autonomic output as a result of actions in two circumventricular structures , the subfornical organ ( SFO ) and area postrema ( AP ) . - pubmed
Through these actions circulating AngII is thus able to influence efferent projections from these CVO which in turn influence the output of hypothalamic cells projecting to the posterior pituitary ( vasopressin secretion ) , nucleus tractus solitarius ( NTS ) , and intermediolateral cell column of the spinal cord ( to influence sympathetic preganglionics ) , and medullary neurons in the NTS . - pubmed
We report a modified ion - pair extraction of interfering substances by tetrahexylammonium chloride ( THAC ) in ethyl acetate in the presence of albumin , and a microtitre plate assay for biliary beta - glucuronidase activity in bile with the substrate p - nitrophenol - glucuronide . - pubmed
Adding albumin improved the recovery of beta - glucuronidase activity to 99.8 % ( CV 1.9 % ) , and 92.2 % of the bilirubin in bile samples was extracted in one step . - pubmed
These results implicate activated calpain II in neurofibrillary degeneration , provide further evidence for the involvement of the calpain system in AD pathogenesis , and imply that neuronal calcium homeostasis is altered in AD . - pubmed
Leachate and soil samples collected from different tillage systems were analyzed for atrazine using gas chromatography ( GC ) and an enzyme - linked immunosorbent assay ( ELISA ) based on magnetic particle technology . - pubmed
Solid - phase extraction ( SPE ) was used to concentrate atrazine residues in leachate samples and soil extracts before GC analysis . - pubmed
Atrazine concentrations determined by GC ranged from 0.1 to 600 micrograms L-1 for water samples and from 1.0 to 700 micrograms kg-1 for soil samples . - pubmed
Atrazine concentrations in 92 leachate samples as determined by ELISA were well - correlated ( R = 0.97 ) with GC levels over the entire concentration range . - pubmed
Where detection methods ( Method 1 versus Method 2 ) were compared on specific extracts ( & lt ; 10 % MeOH ) , good agreement ( R = 0.99 ) was achieved between ELISA and GC values , illustrating that control of extractant concentration is critical in using this assay for atrazine detection in soil . - pubmed
The role of thiamin - dependent enzymes like transketolase and the importance of the NMDA ( N - Methyl - D - Aspartate ) System is discussed . - pubmed
The synthesis of a variety of novel 4 - amido , 4 - carbamoyl and 4 - carboxamido derivatives of 1 -( 8 - chloro-6,11 - dihydro-5H - benzo [ 5,6 ] cyclohepta [ 1,2 - b ] pyridin-11 - yl ) piperazine to explore the SAR of this series of FPT inhibitors is described . - pubmed
The synthesis of the corresponding 3 - bromo analogues resulted in the discovery of the 4 - pyridylacetyl N - oxides 83b ( + /- ) and 85b [ 11S(- ) ] and the 4 - carboxamidopiperidinylacetamido derivative 160b ( + /- ) , all of which exhibited potent FPT inhibition in vitro . - pubmed
Expression of the receptor tyrosine kinase Ret on the plasma membrane is dependent on calcium . - pubmed
Here we show that expression of p170(ret ) is dependent on calcium . - pubmed
Depletion of extracellular calcium completely blocks p170 ( ret ) expression , which is not caused by a decrease in half - life of p170 ( ret ) at the plasma membrane but by a defect in processing of p150 ( ret ) into p170 ( ret ) . - pubmed
We propose that a relatively high concentration of luminal calcium is necessary for the proper folding of Ret in the endoplasmic reticulum . - pubmed
The 4.5 - kb HIAP2 message is contained within eight coding region exons and a single 5'UTR exon . - pubmed
PSD-95 and SAP102 mRNAs were prominently expressed at embryonic day 13 ( E13 ) in the mantle zone of various brain regions , where NMDA receptor NR2B subunit mRNA is expressed at high levels . - pubmed
The S11 cDNA encodes a 376 amino acid protein with a pI of 5.6 and a molecular mass of 42.9 kDa . - pubmed
MTA ( LY231514 , multitargeted antifolate ) represents a new class of folate antimetabolites and inhibits multiple enzymes in the purine and thymidine biosynthetic pathways , including thymidylate synthase , dihydrofolate reductase , and glycinamide ribonucleotide formyl transferase . - pubmed
The recently developed radioligand [ 123I ] FP - CIT is suitable for clinical single - photon emission tomography ( SPET ) imaging of the dopamine ( DA ) transporter in vivo . - pubmed
In vivo [ 123I ] FP - CIT binding to the DA and serotonin transporters was evaluated after subchronic and acute administration of the drugs . - pubmed
The results of this study suggest that dopaminergic medication will not affect the results of DA transporter SPET imaging with [ 123I ] FP - CIT . - pubmed
Among the antagonists for putative melatonin receptors studied , neither luzindole ( melatonin MT1 and MT2 receptor antagonist ) nor prazosin ( melatonin MT3 receptor antagonist ) antagonized the melatonin reversal of morphine tolerance and dependence . - pubmed
Overall , the present observations suggest that the melatonin - induced reversal of morphine tolerance and dependence may involve its ability to suppress nitric oxide synthase ( NOS ) activity . - pubmed
Further , the melatonin - induced reversal of morphine tolerance and dependence is not mediated through its actions via putative melatonin receptors . - pubmed
The agonistic activity of melatonin towards peripheral benzodiazepine receptors may partially contribute to the suppression of morphine dependence but not to the reversal of tolerance to the analgesic activity of morphine . - pubmed
In contrast , Kv4.3 potassium channel alpha subunit mRNA was expressed at equal levels across the ventricular wall . - pubmed
The ShuS protein has no sequence homology with any proteins of known function and its role in heme acquisition has not been determined . - pubmed
It appears , therefore , that ShuS may function as both a heme storage protein , during periods of active heme transport , and as a DNA binding protein to protect the DNA from any ensuing heme mediated oxidative damage . - pubmed
Although there are numerous mechanisms involved in the process of renal disease progression , the renin - angiotensin system plays a major role as determined by the beneficial response to angiotensin I - converting enzyme inhibitors ( ACEIs ) and angiotensin II receptor blockers ( AT II blockers ) of reduced rate of progression in a variety of clinical and experimental renal diseases . - pubmed
Dual role of Fyn in the regulation of FAK + 6,7 by cannabinoids in hippocampus . - pubmed
In hippocampus endocannabinoids modulate synaptic function and plasticity and increase tyrosine phosphorylation of several proteins , including focal adhesion kinase ( FAK ) . - pubmed
Autophosphorylation of FAK on Tyr-397 is generally a critical step for its activation , allowing the recruitment of Src family kinases , and phosphorylation of FAK and associated proteins . - pubmed
Anandamide and 2 - arachidonoylglycerol , two endocannabinoids , and Delta9 - tetrahydrocannabinol , stimulated tyrosine phosphorylation of FAK + 6,7 , a neuronal splice isoform of FAK , on several residues including Tyr-397 . - pubmed
These effects were sensitive to manipulation of cAMP - dependent protein kinase , suggesting that they were mediated by inhibition of a cAMP pathway . - pubmed
PP2 , an Src family kinase inhibitor , prevented the effects of cannabinoids on p130 - Cas and on FAK+6,7 tyrosines 577 and 925 , but not 397 , indicating that FAK autophosphorylation was upstream of Src family kinases in response to CB1 - R stimulation . - pubmed
These findings suggested that the MMP inhibitor , R-94138 , could be used in adjunctive therapy for lymph node metastasis in gastric carcinoma . - pubmed
In contrast , only a limited number of inhibitors of KA receptor activity have been developed , most of which contain quinoxalinedione or decahydroisoquinoline skeletons . - pubmed
The purpose of this study was to examine the effects of fluid replacement on power output ( PO ) , rating of perceived exertion ( RPE ) , heart rate ( HR ) , body weight ( BW ) , urine osmolarity ( Uosm ) , and urine electrolyte concentrations ( [ UNa+ ] , [ UK+ ] , [ UCl - ] ) in physically active men ( n = 4 ) and women ( n = 7 ) . - pubmed
The enzyme tRNA - guanine transglycosylase ( TGT , EC 2.4.2.29 ) catalyzes a posttranscriptional transglycosylation reaction involved in the incorporation of the modified base queuine [ Q , 7 -( 4,5 - cis - dihydroxy-2 - cyclopenten-1 - ylaminomethyl )- 7 - deazaguanine ] into tRNA . - pubmed
coli TGT ( alanine , asparagine , cysteine , and glutamate ) . - pubmed
All four mutant TGTs were able to noncovalently bind tRNA , but only the glutamate mutant was able to form a stable complex with the RNA substrate under denaturing conditions that was comparable to wild type . - pubmed
Furthermore , the glutamate mutant was the only mutant TGT that demonstrated significant activity . - pubmed
Kinetic parameters were determined for this enzyme and shown to be comparable to wild type , revealing that the enzyme is considerably tolerant of the positioning of the carboxylate . - pubmed
Under conditions of high enzyme concentrations and long time courses , the alanine , asparagine , and cysteine mutants showed very low levels ( ca . - pubmed
INTRODUCTION : The main purpose of this contribution is to set today 's situation of technology assessment and point to some organizing and integrative ways with the evolution concern ASL ( Local Health Firm ) . - pubmed
The aim of the work was to describe the actual health status and the extent and seriousness of the affliction before the substitution therapy with opioid receptors agonist -- methadone . - pubmed
In mechanical mixtures of H - ZSM-5 and In2O3 thermal auto - reductive solid - state ion exchange ( AR - SSIE ) was found to proceed upon treatment in high vacuum at 840 K resulting in the incorporation of In+ ions into , and in an increase of the thermal stability of , the zeolitic component . - pubmed
Parallel solution- and solid - phase synthesis of spiropyrrolo - pyrroles as novel neurokinin receptor ligands . - pubmed
The combination of a 3,5 - bis ( trifluoromethyl ) phenyl needle with the spiropyrrolo - pyrrole motive as a privileged GPCR scaffold was the basis for designing a focused combinatorial library targeted towards the neurokinin-1 receptor . - pubmed
A tensile strain applied to the substrate for 15 min significantly increased microtubule ( MT ) assembly by 32 + /- 7 % , with no apparent effect on the cells ' focal adhesions as revealed by immunofluorescence and quantitative analysis of Triton X-100 - insoluble vinculin levels . - pubmed
Disruption of the MT network by prolonged treatments with low doses of either nocodazole or paclitaxel before the application of strain abolished the redistribution of RhoA and Rac in response to the applied forces . - pubmed
We also recorded non - sodium currents from Purkinje cells of both genotypes to test whether the Scn8a mutation induced changes in other ion channels . - pubmed
Hepatic glycogen was assessed by natural abundance 13C nuclear magnetic resonance spectroscopy ( MRS ) . - pubmed
After an oral dosing , blood samples were collected for a period of 60 h. Plasma concentrations of haloperidol were determined using a liquid chromatographic electrospray mass spectrometric ( LC - MS ) method . - pubmed
Several proteins with diverse functions outside primary carbon metabolism , such as the isomerase ROC4 , lipoxygenase 2 involved in jasmonic acid biosynthesis , and a carbonic anhydrase ( CA1 ) , were surprisingly abundant in the range of 0.75 - 1.5 % of the total stromal mass . - pubmed
Two - dimensional difference gel electrophoresis ( DIGE ) in combination with univariate ( Student 's t - test ) and multivariate data analysis , principal component analysis ( PCA ) and partial least squares discriminant analysis ( PLS - DA ) were used to study the anti - inflammatory effects of the beta ( 2 )- adrenergic receptor ( beta ( 2 )- AR ) agonist zilpaterol . - pubmed
This inhibition was counteracted by addition of the beta ( 2 )- AR antagonist propranolol ( LZP ) . - pubmed
The inhibition of MIP-1beta by zilpaterol and the involvement of the beta ( 2 )- AR and cAMP were confirmed using a specific immunoassay . - pubmed
Different analyses were made : light microscopy , scanning electron microscopy ( SEM ) and energy dispersive spectroscopy ( EDS ) , calcium incorporation to newly formed tissue ( alizarin red ) , bone nodule formation ( von Kossa ) , fat aggregate formation ( oil red O ) , collagen type I immunofluorescence , DNA concentrations , alkaline phosphatase ( ALP ) activity , and osteocalcin concentrations . - pubmed
Formation of MT1 / MT2 heterodimers remains to be shown in native tissues but is suggested by the documented co - expression of MT1 and MT2 in many melatonin - sensitive tissues , such as the hypothalamic suprachiasmatic nuclei , retina , arteries , and adipose tissue . - pubmed
Interaction between tyrosineZ and substrate water in active photosystem II . - pubmed
However , there is no direct evidence for the interaction between Tyr(Z ) and substrate water in active Photosystem II ( PSII ) , instead most experiments have been performed on inhibited PSII . - pubmed
EPR measurements observed that the maximum yield of Tyr(Z ) oxidation at cryogenic temperature in the S(0 ) and S(1 ) states was around neutral pH and was essentially pH - independent . - pubmed
Regular insulin secretory oscillations despite impaired ATP synthesis in Friedreich Ataxia patients . - pubmed
Basal secretion of insulin occurs in an oscillating manner presumably triggered by ATP - dependent feedback inhibition of glycolytic flux . - pubmed
In the present study Friedreich Ataxia patients with a presumptive impairment of ATP synthesis in pancreatic beta - cells were evaluated for regularity of basal secretory oscillations of insulin . - pubmed
Aspirin can reduce thrombin generation with the subsequent attenuation of thrombin - mediated coagulant reactions such as factor XIII activation . - pubmed
Aspirin also acetylates lysine residues in fibrinogen resulting in increased fibrin clot permeability and enhanced clot lysis as well as directly promoting fibrinolysis with high - dose aspirin . - pubmed
The variable effectiveness of aspirin in terms of clinical outcomes and laboratory findings , which has been termed aspirin resistance , may be related to these additional antithrombotic effects that are altered when associated with common genetic polymorphisms such as the Leu33Pro beta ( 3 )- integrin or Val34Leu factor XIII mutations . - pubmed
Using Western blotting , PhIP was shown to induce expression of the DNA damage response proteins p53 and p21(WAF1 / CIP1 ) , and to inhibit cell growth while activating G1 cell cycle checkpoint , a consequence of PhIP - induced DNA damage . - pubmed
For the genomic pathway , progesterone interacts with the type - B nuclear progesterone receptor ( nPR - B ) , which decreases expression of contraction - associated genes . - pubmed
For the nongenomic pathway , progesterone interacts with membrane - associated PRs ( mPRs ) to modulate directly intracellular Ca2 + and cyclic adenosine monophosphate ( cAMP ) levels . - pubmed
, PR - A and/or PR - C ) and changes in nPR coregulator levels that together repress the transcriptional activity of PR - B ; and ( 2 ) increased expression of specific mPRs , especially mPRss , which augments contractility by decreasing intracellular cAMP . - pubmed
Functional progesterone withdrawal may be induced by locally produced prostaglandins that alter myometrial nPR expression . - pubmed
( 3 ) As ( 2 ) O ( 3 ) could significantly down - regulate DNA methyltransferase 1 ( DNMT 1 ) , DNMT3A and DNMT3B gene at mRNA level in a dose - dependent manner . - pubmed
Despite treatment with systemic steroids , immunosuppressants , chemotherapy and interferon , progressive deterioration occurred . - pubmed
The effect of several metabolic genotypes on adduct levels were also investigated and higher PhIP and ABP adducts were associated with the rapid NAT2 and/or rapid NAT1 genotypes . - pubmed
In the allopurinol group , serum uric acid levels , GFR , systolic and diastolic blood pressure , and C - reactive protein ( CRP ) levels significantly improved ( P & lt ; 0.05 ) . - pubmed
No correlation was observed between changes in GFR and changes in CRP , or blood pressure in the allopurinol group . - pubmed
FDG - PET / CT finding of high uptake in pulmonary alveolar microlithiasis . - pubmed
Combined positron emission tomography and computed tomography using 18F - FDG ( FDG - PET / CT ) showed the abnormal accumulation of FDG in both lungs with a maximal standardized uptake value of 7.3 . - pubmed
A morphological study of 200 atherosclerotic carotid sinus ( CS ) plaques obtained at carotid endarterectomy revealed the structural components and processes characteristic for severe atherosclerosis ( foci of atheromatosis and edema , necrosis of collagen and elastic fiber , newly formed vessels and hemorrhages of various duration , lipophages and lymphocytes , portions of fibrosis and calcification , covering thinning and ulceration , thrombi , and the contents of plagues ( atheromatous masses , cholesterol crystals , lipophages , calcificates ) on their surface ) . - pubmed
One - pot synthesis of carbazoles by palladium - catalyzed N - arylation and oxidative coupling . - pubmed
One - pot N - arylation and oxidative coupling can be promoted by a common palladium catalyst in the presence of appropriate additives : palladium - catalyzed N - arylation of anilines with aryl triflates under the standard conditions followed by addition of acetic acid under oxygen or air atmosphere afforded various types of functionalized carbazoles in good to excellent yields . - pubmed
Evidence of Maillard reactivity was suggested using colorimetry , o - phthaldialdehyde assays , and sodium dodecyl sulfate PAGE followed by glycoprotein staining . - pubmed
Resultant glycoprotein dispersions exhibited altered functionality , in which case steady shear and small amplitude oscillatory rheology parameters were shown to be dependent on the specific reducing sugar present . - pubmed
The decrease of pH in the anaerobic self - fermentation of sterilized sludge was very lower ( from 6.81 to 6.56 ) because NH ( 4 )(+ ) produced by degradation of proteins could neutralize organic acids produced in the process . - pubmed
A new PEG-1000 - based dicationic ionic liquid exhibiting temperature - dependent phase behavior with toluene and its application in one - pot synthesis of benzopyrans . - pubmed
A new recyclable temperature - dependant phase - separation system comprised of PEG-1000 - based dicationic acidic ionic liquid and toluene was developed and used in one - pot three - component condensation to prepare benzopyrans in excellent yields ( up to 93 % ) . - pubmed
Our data provide mechanistic evidence for the first time showing that DIM potentiates the killing of pancreatic cancer cells by down - regulation of constitutive as well as drug - induced activation of NF - kappaB and its downstream genes ( Bcl - xL , XIAP , cIAP , and survivin ) . - pubmed
This was accompanied by loss of phospho - p65 and down - regulation of NF - kappaB activity and its downstream genes ( Bcl - xL , survivin , and XIAP ) , which correlated with reduced cell proliferation ( as assessed by Ki-67 immunostaining of tumor specimens ) and evidence of apoptosis [ as assessed by poly ( ADP - ribose ) polymerase cleavage and terminal deoxynucleotidyl transferase - mediated dUTP nick end labeling staining ] . - pubmed
These results provide strong in vivo evidence in support of our hypothesis that DIM could abrogate chemotherapeutic drug ( cisplatin , gemcitabine , and/or oxaliplatin)-induced activation of NF - kappaB , resulting in the chemosensitization of pancreatic tumors to conventional therapeutics . - pubmed
Taken together , these findings strengthen the argument that besides mTOR - inhibitors , such as rapamycin analogues , p27 and CDKs could also be considered targets for hamartoma therapeutics in tuberous sclerosis . - pubmed
Complex 1 displays bright orange luminescence in dichloromethane solution , arising from pi ( phenylacetylide )--& gt;pi *( Me ( 3 ) SiC [ triple bond ] CbpyC [ triple bond ] CSiMe ( 3 ) ) ( 3 ) LLCT and d(Pt)-->pi *( Me ( 3 ) SiC [ triple bond ] CbpyC [ triple bond ] CSiMe ( 3 ) ) ( 3 )MLCT triplet states which are supported by DFT calculation . - pubmed
There is a need to develop oral models of significant liver injury and to evaluate the possible role of CYP2E1 in ethanol actions in such models . - pubmed
These results show that CYP2E1 plays a major role in ethanol - induced fatty liver and oxidant stress . - pubmed
Then clinical symptoms , and levels of body mass index ( BMI ) , obesity degree , alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , gamma - glutamyl transpeptidase ( gamma - GT ) , total cholesterol ( TC ) , triglyceride ( TG ) and CT ratio of liver and spleen before and after treatment were compared between two groups . - pubmed
This process is initiated by the recognition of the Tyr- and di - Leu - based endocytic motifs of CFTR by the AP-2 adaptor complex , leading to the formation of clathrin - coated vesicles and the channel delivery to sorting / recycling endosomes . - pubmed
Antioxidant indicators ( CAT , MDA and T - AOC ) and neurotransmitters ( NO , Glu and Ach ) in cerebra were measured . - pubmed
CAT activities , MDA , NO and Glu contents were decreased in SIF H group only . - pubmed
Tyrosine phosphorylation of the Galpha - interacting protein GIV promotes activation of phosphoinositide 3 - kinase during cell migration . - pubmed
We demonstrate that GIV is a tyrosine phosphoprotein that directly binds to and activates phosphoinositide 3 - kinase ( PI3 K ) . - pubmed
Upon ligand stimulation of various receptors , GIV was phosphorylated at tyrosine-1764 and tyrosine-1798 by both receptor and non - receptor tyrosine kinases . - pubmed
These phosphorylation events enabled direct binding of GIV to the amino- and carboxyl - terminal Src homology 2 domains of p85alpha , a regulatory subunit of PI3 K ; stabilized receptor association with PI3 K ; and enhanced PI3 K activity at the plasma membrane to trigger cell migration . - pubmed
Tyrosine phosphorylation of GIV and its association with p85alpha increased during metastatic progression of a breast carcinoma . - pubmed
These results suggest a mechanism by which multiple receptors activate PI3 K through tyrosine phosphorylation of GIV , thereby making the GIV - PI3 K interaction a potential therapeutic target within the PI3K - Akt pathway . - pubmed
This nanoparticle - dODN bioconjugate ( gammaFe ( 2 ) O ( 3 )@ dODN ) was designed to target the signal transducer and activator of transcription 3 , STAT3 , a key regulator of cell survival and proliferation . - pubmed
Lectins are also one of the promising candidates for useful therapeutic agents because they can recognize the specific carbohydrate structures such as proteoglycans , glycoproteins , and glycolipids , resulting in the regulation of various cells via glycoconjugates and their physiological and pathological phenomenon through the host - pathogen interactions and cell - cell communications . - pubmed
PURPOSE : Amuvatinib is a multi - targeted tyrosine kinase inhibitor with activity that also disrupts DNA damage repair through suppression of homologous recombination protein Rad51 . - pubmed
GE activity was highly specific and hydrolysed the peptide bond predominantly on the carboxy side of Glu residues while hydrolysis on the carboxyl side of Asp residues was also observed . - pubmed
Src kinase is a direct target of apigenin against UVB - induced skin inflammation . - pubmed
Apigenin inhibited UVB - induced cyclooxygenase-2 ( COX-2 ) expression , which is a well - known key mediator of inflammation and cancer , and restored the upstream stimulatory factor level in JB6 P+ cells . - pubmed
Immunoblot and kinase assay data demonstrate that Src activity was attenuated by apigenin , and this led to subsequent inhibition of UVB - induced phosphorylation of epidermal growth factor receptor , mitogen - activated protein kinases and Akt signaling . - pubmed
In addition , in vitro pull - down assays revealed that apigenin binds Src in an adenosine triphosphate - competitive manner . - pubmed
Collectively , these findings suggest that apigenin exerts potent chemopreventive activity against UVB - induced skin inflammation primarily by targeting Src . - pubmed
The synaptic adhesion molecule SynCAM 1 contributes to cocaine effects on synapse structure and psychostimulant behavior . - pubmed
SynCAM 1 additionally contributes to the structural remodeling of NAc synapses in response to the psychostimulant cocaine . - pubmed
Specifically , we find that cocaine administration increases the density of stubby spines on medium spiny neurons in NAc , and that maintaining this increase requires SynCAM 1 . - pubmed
This study investigated the role of Nrf2 in mediating PHB - induced protection against colitis and expression of the antioxidant response element ( ARE)-regulated antioxidant genes heme oxygenase-1 ( HO-1 ) and NAD ( P ) H quinone oxidoreductase-1 ( NQO-1 ) . - pubmed
Suberoylanilide hydroxamic acid and anacardic acid were used to inhibit histone deacetylase and histone acetyltransferase , respectively . - pubmed
Finally , the selective CXCR2 antagonist SB225002 was administered intrathecally to reveal the function of spinal CXCR2 receptors after hind paw incision . - pubmed
Overall , acetylated histone H3 at lysine 9 was increased in spinal cord tissues after incision , and enhanced association of acetylated histone H3 at lysine 9 with the promoter regions of CXCR2 and keratinocyte - derived chemokine ( CXCL1 ) was observed as well . - pubmed
Epitaxial monolayer MoS2 on mica with novel photoluminescence . - pubmed
Herein , we have successfully grown predominantly monolayer MoS2 on an inert and nearly lattice - matching mica substrate by using a low - pressure chemical vapor deposition method . - pubmed
Photoluminescence ( PL ) measurements indicate strong single - exciton emission in as - grown MoS2 and room - temperature PL helicity ( circular polarization ~0.35 ) on transferred samples , providing straightforward proof of the high quality of the prepared monolayer crystals . - pubmed
For cytokine secretion , alpha - mangostin ( 1 , 2 and 4 microg / mL ) did not significantly induce either proinflammatory cytokines ( i.e. - pubmed
The combination of alpha - mangostin ( 1 , 2 and 4 microg / mL ) and ConA did not significantly alter the relative difference of TNF - alpha and IL-1beta compared with ConA - treated PBMCs ; however , these combinations could significantly decrease the relative difference of IL-2 compared with ConA - treated PBMCs . - pubmed
Recent evidence supports the hypothesis that tinnitus is related to neuronal hyperactivity in auditory brain regions , and consequently drugs that increase GABAergic neurotransmission in the CNS , such as the GABA ( B ) receptor agonist L - baclofen , may be effective as a treatment . - pubmed
It also lowered plasma triglyceride ( 4.0 + - 1.6 mmol / L vs 2.6 + - 1.2 mmol / L , p<0.05 ) , but there were no significant changes in body weight , high density lipoprotein cholesterol , apolipoprotein A or apolipoprotein B. - pubmed
Functional assays and measurements of cGMP , reactive - oxygen species ( ROS ) , and sGC protein expression in USM were determined . - pubmed
Oxidation of sGC with ODQ [ 1H -[ 1,2,4 ] oxadiazolo [ 4,3 - a ] quinoxalin-1 - one ] ( 10 muM ) potentiated BAY 60 - 2770 - induced USM responses in the lean group . - pubmed
However , post hoc analysis showed that MBSR decreased the proportion of participants with at least one flare - up among those with top tertile urinary cortisol and baseline perceived stress ( 30 vs. 70 % ; p & lt ; 0.001 ) . - pubmed
GSK1265744 ( GSK744 ) is an integrase strand - transfer inhibitor that has been formulated as a long - acting ( LA ) injectable suitable for monthly to quarterly clinical administration . - pubmed
There is an inverse relationship between indirect bilirubin levels and advanced liver fibrosis caused by CHC genotype 1b . - pubmed
Secondary end points were presence of early seizures ( 0 to 7 days post - TBI ) or late seizures ( 8 days post - TBI to phone interview ) , use of anticonvulsant medication when interviewed , medication - related hospital complications , and a summary of phenytoin ( PHT ) and LEV dosing regimens . - pubmed
Seven - point home blood glucose monitoring over a 7 - day period in each trimester demonstrated higher fasting and postprandial glycemic excursions in the first trimester of GCK pregnancies when compared to HNF-1alpha pregnancies ( fasting 104 [ 90 - 115 ] mg / dL vs 84 [ 77 - 88 ] mg / dL ; P = .01 and postprandial 154 [ 135 - 196 ] mg / dL vs 111 [ 100 - 131 ] mg / dL ; P = .04 ) despite insulin treatment . - pubmed
INTRODUCTION : The adenosine triphosphate - binding cassette transporter A1 ( ABCA1 ) is a major cholesterol transporter highly expressed in the liver and brain . - pubmed
RESULTS : After transgenesis , the proliferation of EPCs were increased , and the secretion of NO , LDH , iNOS by EPCs were significantly increased compared to the non - transgenesis group . - pubmed
Donor and recipient demographic data , preoperative BMI , blood C - reactive protein ( CRP ) and adiponectin levels , perivascular adipose tissue ( PAT ) samples and recipient blood creatinine levels were analyzed . - pubmed
We show the REMD simulation results of a mixed ( POPC / DMPC ) lipid bilayer as a real application using GENESIS . - pubmed
ASH was induced by ethanol ( 3 times ; 5 g / kg ; 12 h intervals , via gavage ) , followed by a single dose of lipopolysaccharide ( LPS ; 10 mg / kg ; i.p . ) - pubmed
Reactive oxygen species formation , malondialdehyde levels , myeloperoxidase activities and transforming growth factor beta1 ( TGF - beta1 ) and collagen 1alpha1 ( COL1A1 ) expressions were observed to decrease . - pubmed
In the present study , danshensu which is a selected component of traditional oriental medicine ( TOM ) compound was found to reduce the radioresistance of NSCLC by inhibiting the nuclear factor - kappaB ( NF - kappaB ) pathway . - pubmed
Taken together , this study shows that danshensu significantly reduces MAOB activity and attenuates NF - kappaB signaling to elicit the radiosensitization of NSCLC . - pubmed
Recently , hepcidin , an antimicrobial - like peptide hormone , has evolved as the master regulator of iron homeostasis . - pubmed
Iron parameters including ( serum iron , ferritin , transferrin , and total iron binding capacity [ TIBC ] ) were also measured . - pubmed
We found that AIS cases had higher serum iron , ferritin , and IL6 levels compared to the control group ( all P & lt ; 0.01 ) . - pubmed
Serum hepcidin showed significant positive correlations with serum iron , transferrin saturation , ferritin , and IL6 ( r = 0.375 , P & lt ; 0.05 ; r = 0.453 , P & lt ; 0.05 ; r = 0.687 , P & lt ; 0.01 ; r = 0.515 , P & lt ; 0.01 ; respectively ) . - pubmed
RESULTS : We verified that chronic administration of escitalopram decreased the activities of complexes I and II - III in cerebellum , hippocampus , striatum and posterior cortex whereas prefrontal cortex was not affected . - pubmed
However , chronic administration of escitalopram did not affect complex IV and enzymes of Krebs cycle activities as well as creatine kinase . - pubmed
The laboratory prediction model composed of fasting plasma glucose and hemoglobin A1c levels showed a significantly higher AROC ( 0.838 , P & lt ; 0.001 ) than the KRS . - pubmed
The total organic carbon content measurements confirmed the results obtained by LC / MS . - pubmed
Prevalence and significance of HBeAg and isolated anti - HBc in several institutions for the mentally retarded in the autonomous community of madrid . - pubmed
Of the total , 28 ( 6.8 % ) were HBsAg positive , and of these , 16 ( 57.1 % ) had a positive test for the Hepatitis Be antigen ( HBeAg ) . - pubmed
Proportionately more Down syndrome chronic carriers of HBsAg acquired the persistent Hepatitis Be antigen ( HBeAg ) than non - Down syndrome patients 15 ( 65.2 % ) vs. 1 ( 20 % ) . - pubmed
The presence of HBeAg was correlated with abnormal liver function and high titres of HBsAg . - pubmed
Testing for the IgM antibody to the hepatitis B core antigen ( IgM AntiHBc ) facilitated the identification of acute and chronic hepatitis infection in both RI and NRC individuals . - pubmed
Targeting BCR - ABL+ stem / progenitor cells and BCR - ABL - T315I mutant cells by effective inhibition of the BCR - ABL - Tyr177 - GRB2 complex . - pubmed
Interestingly , new pre - clinically - validated analogs of Icaritin ( SNG162 and SNG1153 ) , which target abnormal ERalpha36 activity , inhibit cell growth and induce apoptosis of BCR - ABL+ leukemic cells , particularly BCR - ABL - T315I mutant cells . - pubmed
Assessment of Efficacy and Irritation of Ingenol Mebutate Gel 0.015 % Used With or Without Dimethicone Lotion for Treatment of Actinic Keratosis on the Face . - pubmed
Consistent with the location of the CSPG4 - binding site on TcdB , we show that the anti - TcdB antibody bezlotoxumab , which binds partially within the first three short repeats , prevents CSPG4 binding to TcdB. - pubmed
Independently from the angle , DIP induced decrements in MVC , RFD , and RMS of EMG and MMG signals and lengthened the EMD components in agonist muscles ( P<0.05 ) . - pubmed
GC / EI - MS and GC / ECNI - MS mass spectra of the five PMPs were studied with regard to fragmentation pattern and individual responses which were strongly affected by the presence ( or absence ) of Br in alpha - position(s ) . - pubmed
A key enzyme of the shikimate pathway , 5 - enolpyruvylshikimate-3 - phosphate synthase ( EPSPS ; EC 2.5.1.19 ) , is the known target of the widely used herbicide glyphosate . - pubmed
The aim of this work was to study the pleiotropic effects of ( i ) EPSPS increased transcript abundance due to gene copy number variation ( CNV ) and of ( ii ) glyphosate application on the aromatic amino acid ( AAA ) and branched chain amino acid ( BCAA ) synthesis pathways . - pubmed
Hydroponically grown glyphosate sensitive ( GS ) and glyphosate resistant ( GR ) plants were treated with glyphosate 3 days after treatment . - pubmed
In absence of glyphosate treatment , high EPSPS gene copy number had only a subtle effect on transcriptional regulation of AAA and BCAA pathway genes . - pubmed
In contrast , glyphosate treatment provoked a general accumulation of the transcripts corresponding to genes of the AAA pathway leading to synthesis of chorismate in both GS and GR . - pubmed
After chorismate , anthranilate synthase transcript abundance was higher while chorismate mutase transcription showed a small decrease in GR and remained stable in GS , suggesting a regulatory branch point in the pathway that favors synthesis toward tryptophan over phenylalanine and tyrosine after glyphosate treatment . - pubmed
Importantly , this upregulation was glyphosate dose dependent and was observed similarly in both GS and GR populations . - pubmed
palmeri GR populations has no major pleiotropic effect on the expression of AAA biosynthetic genes , even in response to glyphosate treatment . - pubmed
As a result , a distinct colorimetric signal is produced by the ABTS oxidation promoted by the peroxidase mimicking activity of the released G - quadruplex DNAzymes . - pubmed
Communication : Mode - dependent excited - state lifetime of phenol under the S1 / S2 conical intersection . - pubmed
The reaction barrier formed by the conical intersection between the optically bright S1 ( pipi * ) state and the dissociative S2 ( pisigma * ) state does not inhibit O - H bond rupture even though the excitation energy is below the barrier height . - pubmed
To examine the O - H bond - rupture dynamics in association with the initial excited vibrational modes , we used a picosecond laser to investigate the vibrational - mode - dependent excited - state lifetime of phenol under the S1 / S2 conical intersection . - pubmed
Unexpectedly short lifetimes were observed in the S1 state for a '' symmetric vibrational modes ( including v4 , v16a , tauOH , and v5 ) . - pubmed
In this work , a wide range of 1,2,3 - triazole - chromenone carboxamides were designed , synthesized , and evaluated for their cholinesterase inhibitory activity . - pubmed
Among them , N -( 1 - benzylpiperidin-4 - yl )- 7 -(( 1 -( 3,4 - dimethylbenzyl )- 1H-1,2,3 - triazol-4 - yl ) methoxy )- 2 - oxo-2H - chromene-3 - carboxamide ( 11b ) showed the best acetylcholinesterase inhibitory activity ( IC50 = 1.80 microM ) , however , it was inactive toward butyrylcholinesterase . - pubmed
Potassium level before dialysis ( OR = 0.60 ) , parathyroid hormone ( OR = 0.99 ) , and higher Kt / V ( OR = 0.28 ) were found to be an advantage for survival . - pubmed
The antibacterial effect of fluoxetine was determined by a broth microdilution method ( the M07 - A9 method of the Clinical and Laboratory Standard Institute ) and flow cytometry techniques in which the probable mechanism of action of the compound was also assessed . - pubmed
Body mass index ( BMI ) , glycated hemoglobin ( HbA1c ) , fasting blood glucose ( FBG ) , and lipid level , as well as Mini - Mental State Examination and Berg Balance Scale scores , were measured at baseline and postintervention . - pubmed
In our study , we detected that oxidant parameter values and cytokine levels increased in the negative control group , but total antioxidant status reduced , whereas , cytokine and oxidant parameter levels were significantly reduced due to low and high doses of caftaric acid administration . - pubmed
Vitamin B12 modulates Parkinson ' s disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection . - pubmed
However , nearly all inhibitors are ATP competitive - some with unwanted side effects and unclear clinical outcome - alternative types of LRRK2 inhibitors are lacking . - pubmed
Herein we identify 5 '- deoxyadenosylcobalamin ( AdoCbl ) , a physiological form of the essential micronutrient vitamin B12 as a mixed - type allosteric inhibitor of LRRK2 kinase activity . - pubmed
Multiple assays show that AdoCbl directly binds LRRK2 , leading to the alterations of protein conformation and ATP binding in LRRK2 . - pubmed
STD - NMR analysis of a LRRK2 homologous kinase reveals the contact sites in AdoCbl that interface with the kinase domain . - pubmed
Furthermore , we provide evidence that AdoCbl modulates LRRK2 activity through disrupting LRRK2 dimerization . - pubmed
Our study uncovers vitamin B12 as a novel class of LRRK2 kinase modulator with a distinct mechanism , which can be harnessed to develop new LRRK2 - based PD therapeutics in the future . - pubmed
One hydrochlorofluorocarbon and two hydrofluorocarbons ( HCFC-22 , HFC-125 , and HFC-152a ) were measured in air samples at the Cape Point observatory ( CPT ) , South Africa , during 2017 . - pubmed
The components analysed were major STP components such as water , nicotine , sugars , humectants , sodium ions , chloride ions and ash . - pubmed
The HA protein of viruses from each cluster showed a different number of basic amino acids ( n = 5 - 7 ) in the cleavage site , and six different patterns at the predicted N - glycosylation sites . - pubmed
The Dephosphorylation of p70S6 ( Thr389 ) Kinase as a Marker of L - Glutamate - Induced Excitotoxicity Related to Diabetes Disturbances - an Unconventional In Vitro Model . - pubmed
The three reagents were used in different concentrations to create the model of excitotoxicity related to diabetes disturbances : L - glutamate ( 2.5 mM ; 10 mM ) , glucose ( 150 mM ; 200 mM ) , and insulin ( 0.093 mM ; 0.371 mM ) . - pubmed
The results we obtained demonstrated the increased phosphorylation of p70S6 K in Thr389 residue in almost all combinations of reagents , except for those including the highest concentration of L - glutamate , in which dephosphorylation was confirmed . - pubmed
This confirms the inhibition of mTOR kinase and suggests that p70S6 K ( Thr389 ) plays a functional role in the regulation of the signaling pathway in excitotoxicity related to diabetic disturbances . - pubmed
Control of the redox potential of lithium terephthalate Li2TP anode material is demonstrated by functionalizing its terephthalate backbone with an electron - donating amino group ; this lowers - as intended - the redox potential of Li2TP by 0.14 V. The two Li - organic electrode materials , Li2TP and Li2TP - NH2 , are fabricated as crystalline thin films from gaseous precursors using the atomic / molecular layer deposition ( ALD / MLD ) technique . - pubmed
From galvanostatic cycling experiments and post - mortem XRD and SEM analysis , the issue with Li2TP - NH2 is revealed to be in the morphology changes occurring during the discharge / charge cycling . - pubmed
Only 79 % of Pt mine workers , used their respiratory protective equipment ( RPE ) , sixty - five percent were exposed to work shifts exceeding the recommended eight hours and 8.8 % had been employed for more than ten years . - pubmed
The Pt miners had increased health risks due to accumulated low levels of dust exposure and lack of usage of RPE . - pubmed
Antidepressant - Like Effects and Cognitive Enhancement of Coadministration of Chaihu Shugan San and Fluoxetine : Dependent on the BDNF - ERK - CREB Signaling Pathway in the Hippocampus and Frontal Cortex . - pubmed
Then , the depression - like behavior and cognitive function were evaluated by the sucrose preference test ( SPT ) , forced swimming test ( FST ) , open field test ( OFT ) , and Y - maze test . - pubmed
What is more , the antidepressant mechanism of CSS and coadministration of CSS and FLU were studied through BDNF mRNA , ERK mRNA , CREB mRNA , BDNF , p - ERK / ERK , and p - CREB / CREB levels in the hippocampus and frontal cortex by Western blot and RT - PCR . - pubmed
Finally , we found that both CSS and coadministration of CSS and FLU play an antidepressant role , which may be due to the regulation of the BDNF / ERK / CREB signaling pathway in the hippocampus and frontal cortex . - pubmed
We used this tryptophan to determine the binding affinity of the unlabeled IkappaBalpha peptide to 14 - 3 - 3 via tryptophan fluorescence decay measurements . - pubmed
MATERIALS AND METHODS : We studied the anti - HCC effect of FH535 and a novel derivative Y3 , on proliferation , mitochondrial function and cellular metabolism focusing on the three key substrates , glutamine , glucose , and fatty acids . - pubmed
These sulfonamides , FH535 and Y3 , targeted glutamine and fatty acid metabolism and caused HCC cell reprograming towards the preferential use of glucose and the glycolytic pathway . - pubmed
CONCLUSIONS : FH535 , and Y3 , demonstrated potent anti - HCC activity by targeting OXPHOS , increasing dangerous levels of ROS and reducing ATP production . - pubmed
These sulfonamides target glutamine and FA metabolic pathways significantly increasing the cellular dependency on glycolysis . - pubmed
OBJECTIVE : This study aimed to determine how the GABAa and GABAb receptors of the central nucleus of the amygdala ( CeA ) regulate the compulsive cocaine - seeking behavior . - pubmed
The compulsive cocaine - seeking behavior was measured as the cocaine - seeking behavior in the face of footshock punishment . - pubmed
The role of the GABA receptors of CeA in the regulation of such behavior was determined by measuring the dose - dependent effects of the GABAa agonist muscimol or the GABAb agonist baclofen bilaterally microinjected into the CeA on the punished cocaine - seeking behavior . - pubmed
CONCLUSION : These data indicate that the CeA GABAa receptors play a key role in the regulation of the compulsive cocaine - seeking behavior and suggest that an increase in the function of the GABAa receptors possibly induced by cocaine or genetic factors may be an important mechanism involved in the development of or vulnerability to the compulsive cocaine use and addiction . - pubmed
Characterization of four BCHE mutations associated with prolonged effect of suxamethonium . - pubmed
Here , we report the characterization of four BCHE mutations associated with prolonged effect of suxamethonium ( amino acid numbering based on the matured enzyme ): p.20delValPheGlyGlyThrValThr , p. Leu88His , p. Ile140del and p. Arg386Cys . - pubmed
Cartilage was treated enzymatically to extract collagen , analyzed for 14C content , and year at formation was determined from historical atmospheric 14C concentrations . - pubmed
The target of rapamycin ( TOR ) is an evolutionarily - conserved serine / threonine kinase that senses and integrates signals from the environment to coordinate developmental and metabolic processes . - pubmed
A decrease in serum phosphate ( PO43- ) levels , which in most cases is asymptomatic , has been reported with IV iron preparations . - pubmed
The face shield frames were manufactured using the 3DVerkstan design and were coupled with an acetate sheet to assemble a complete face shield . - pubmed
We highlight that the MeCP2B isoform is required for correct higher - order PCH organization , and underline involvement of the methyl - binding and transcriptional repression domains . - pubmed
The administration of monosodium glutamate ( MSC ) to chick embryos at 24 hours did neither produce gross developmental defects nor influence growth by 72 hours total incubation . - pubmed
Zinc ions were shown to reversibly inhibit the activity of Pseudomonas protease . - pubmed
To characterize the site or sites of initiation of this tRNA transcription , rifampicin - resistant complexes of RNA polymerase , DNA , and either ATP ( UTP and CTP ) or GTP ( UTP and CTP ) were formed , and tRNA was transcribed from these stabilized sites . - pubmed
It is concluded that the initiation of tRNA transcription can occur , without accessory factors , with the insertion of ATP at a single or a few closely spaced sites , and that the tRNAs encoded by the bacteriophage T4 are present in a single operon . - pubmed
thermophilus PheRS , a heterotetrameric ( alpha 2 beta 2 ) , class II aminoacyl - tRNA synthetase . - pubmed
Intraperitoneal injection of caerulein did not change the basal c - fos and zif / 268 expression . - pubmed
Pretreatment with caerulein suppressed the PTZ - induced c - fos and zif / 268 expression . - pubmed
Nonphorbol tumor promoters okadaic acid and calyculin - A induce membrane translocation of protein kinase C. - pubmed
The cell - permeable inhibitors of type 1 and 2A protein phosphatases , okadaic acid and calyculin - A , induced a redistribution of protein kinase C ( PKC ) activity and immunoreactivity ( 40 to 60 % ) from cytosol to membrane in some cell types . - pubmed
Calyculin - A was 100 - fold more potent than okadaic acid and required only 5 to 10 nM concentrations to induce this PKC translocation . - pubmed
Taken together these results suggest that although the okadaic acid class of tumor promoters and phorbol ester - related agents bind to two different cellular receptors having counteracting enzymic activities , they share a common mechanism of action , namely the induction of cytosol to membrane translocation of PKC . - pubmed
A Kd of 1.72 + /- 0.12 nM for hSBP showed that the receptor binding site was effective for both androgen - carrier molecules . - pubmed
The receptor complex was also abolished in the presence of a 100 - fold excess of either unlabelled rABP or unlabelled hSBP , or in the presence of 20 mM EDTA . - pubmed
The intermediate V is then reduced to 1 - chloro-3,4 - dihydroxybenzene ( II ) , and the latter is methylated to form 1 - chloro-3,4 - dimethoxybenzene ( VI ) . - pubmed
VI in turn is oxidized by LiP to generate chloride and 2 - methoxy-1,4 - benzoquinone ( VII ) , which is reduced to 2 - methoxy-1,4 - dihydroxybenzene ( IV ) . - pubmed
IV is oxidized by either LiP or MnP to generate 4 - hydroxy-1,2 - benzoquinone , which is reduced to 1,2,4 - trihydroxybenzene ( III ) . - pubmed
This intermediate is also generated during the LiP- or MnP - catalyzed oxidation of the intermediate chlorocatechol ( II ) . - pubmed
The former is recycled by reduction and methylation reactions to generate an intermediate which is also a substrate for peroxidase - catalyzed oxidation , leading to the removal of a second chlorine atom . - pubmed
The effect of a single dose of carbon tetrachloride ( CT ) , 1,2 - dichloroethane ( DCE ) and the mixture on liver toxicity was investigated . - pubmed
Several of the bands identified by SDS - PAGE were composed of glycoproteins or carbohydrates as determined by their affinity for concanavalin A. - pubmed
Four of the antigenic determinants recognized by patient sera reacted with the concanavalin A - peroxidase stain , indicating that they are composed of glycoproteins or carbohydrate . - pubmed
These CO doses produced HbCO concentrations of 12 , 24 , 35 , 45 , and 60 % , respectively , at 30 min postinjection . - pubmed
The susceptibility of a variety of oligosaccharides to endo - beta - N - acetylglucosaminidase D was investigated . - pubmed
The oligosaccharides having the structures of Man alpha 1 - --- 6 ( GlcNAc beta 1 - --- 4Man alpha 1 - --- 3 ) Man beta 1 - --- 4GlcNAc beta 1 - --- 4 (+/- Fuc alpha 1 - --- 6 ) GlcNAcOT , derived from complex type triantennary sugar chains , released + /- Fuc alpha 1 - --- 6GlcNAcOT upon incubation with the enzyme at almost the same rate as Man alpha 1 - --- 6 ( Man alpha 1 - --- 3 ) Man beta 1 - --- 4GlcNAc beta 1 - --- 4GlcNAcOT . - pubmed
These results indicated that endo - beta - N - acetylglucosaminidase D does not require the presence of a free hydroxyl group at the C-4 position of the alpha - mannosyl residue of the trisaccharide glycon : Man alpha 1 - --- 3Man beta 1 - --- 4GlcNAc beta 1 - --- . - pubmed
Studies with 125I - labeled NIDS protein indicated that the main precipitate on 2 - dimensional immunoelectrophoresis was associated with SDS - polyacrylamide gel components of molecular weight 15,000 and 30,000 . - pubmed
Oral ( 100 g ) or intravenous ( 0.5 g X kg-1 ) glucose was used as a stimulus for insulin secretion in both studies . - pubmed
Neither acute nor chronic cimetidine administration modified insulin secretion and glucose tolerance . - pubmed
These data are consistent with the idea that H2 - receptors are not involved in the insulinogenic effect of glucose . - pubmed
The optimal dose for induction , as measured by the increase of the number of cells containing sheep erythrocyte receptors ( E - rosette assay ) , is between 8 and 16 nM 12 - O - tetradecanoylphorbol 13 - acetate ( TPA ) , although there were significant increases of E - rosette - positive ( E(+ ) ) cells at concentrations as low as 1.6 nM TPA . - pubmed
Finally , we have shown that TPA also affected another differentiation marker , the loss of the enzyme terminal deoxyribonucleotidyl transferase . - pubmed
The sequence of the 89 - residue form of HTa is : H2N - Val - Gly - Ile - Ser - Glu - Leu - Ser - Lys - Glu - Val - Ala - Lys - Lys - Ala - Asn - Thr - Thr - Gln - Lys - Val - Ala - Arg - Thr - Val - Ile - Lys - Ser - Phe - Leu - Asp - Glu - Ile - Val - Ser - Glu - Ala - Asn - Gly - Gl y - Gln - Lys - Ile - Asn - Leu - Ala - Gly - Phe - Gly - Ile - Phe - Glu - Arg - Arg - Thr - Gln - Gly - Pro - Arg - L ys - Ala - Arg - Asn - Pro - Gln - Thr - Lys - Lys - Val - Ile - Glu - Val - Pro - Ser - Lys - Lys - Lys - Phe - Val - Phe - Arg - Ala - Ser - Ser - Lys - Ile - Lys - Tyr - Gln - Gln - COOH The molecular weight calculated from the sequence is 9,934 . - pubmed
Another form of HTa probably differs only by the presence of an additional residue ( methionine ) at the NH2 terminus ( the calculated molecular weight of this form is 10,065 ) . - pubmed
The relationship to renin - angiotensin system and electrolyte metabolism ( author 's transl ) ] . - pubmed
The author is of the opinion that the renin - angiotensin - aldosterone system might be related to these changes during respiratory acidosis . - pubmed
These reports prompted me to question whether the renin - angiotensin - aldosterone system was related to the pathophysiological evidence concerning the respiratory acidosis . - pubmed
It is generally accepted that plasma aldosterone concentration is controlled by ( 1 ) the renin - angiotensin system , ( 2 ) ACTH and ( 3 ) serum potassium . - pubmed
Therefore , the purpose of this experiment was to investigate the roles of the renin - angiotensin system and electrolyte metabolism on plasma aldosterone concentration during acute respiratory acidosis . - pubmed
The increase of plasma aldosterone concentration was delayed and blurred , in contrast with a significant increase of plasma renin activity , during the acute respiratory acidosis , due to delayed time course of angiotensin II stimulation and also the expected changes in the concentration of angiotensin II receptors on the zona glomerulosa in the adrenal cortex . - pubmed
There was a possibility that the increase in the reabsorption of sodium and water in the proximal tubules , and the increase of ADH secretion during the acute respiratory acidosis , could be related to a decrease in excreted sodium and potassium as well as urine volume . - pubmed
These results suggest that the changes of plasma aldosterone concentration may be affected partially by renin - angiotensin system but not by serum potassium , and plasma aldosterone should be a minimal determinant on the electrolyte metabolism during respiratory acidosis with 10 % CO2 inhalation . - pubmed
Specific DV antigen was revealed by the fluorescent antibody ( FA ) technique in cells located in the infected tissues . - pubmed
Factor 2 was negatively correlated with UNaV : its correlations with plasma protein concentration , haematocrit , blood pressure , glomerular filtration rate and renal plasma flow implied that it represented post - glomerular plasma protein concentration and its influence on sodium reabsorption . - pubmed
The time course of haemoglobin autoxidation was studied under various conditions at 37 degrees C , and the changes in oxyhaemoglobin , intermediate haemoglobins and methaemoglobin during the reaction were analysed by isoelectric focusing on Ampholine / polyacrylamide - gel plates . - pubmed
Under various conditions ( 10 mM - phosphate buffer , 10 mM - phosphate buffer with 0.1 M - phosphate buffer , 10 mM - phosphate buffer with 0.1 M - NaCl , and 10 mM - phosphate buffer with 0.5 mM - inositol hexaphosphate ; pH range 6.6 - 7.8 each case ) , the intermediate haemoglobins were found to be present as ( alpha 2 + beta 3+)2 and ( alpha 3 + beta 2+)2 valency hybrids from their characteristic positions on electrophoresis . - pubmed
The modes of the quantitative changes in oxyhaemoglobin , intermediate haemoglobins , and methaemoglobin were very similar in all the media used except for the inositol hexaphosphate addition . - pubmed
In the presence of inositol hexaphosphate , the autoxidation rates were considerably accelerated , and the modes of the changes in the haemoglobin derivatives were also considerably altered ; the effects of this organic phosphate were maximal at acidic pH and minimal at alkaline pH. - pubmed
Preliminary analysis has shown possible evidence of benefit for both these clinical indications , and several other glutamate antagonists are now being evaluated for these indications in Phase II trials . - pubmed
Inhibitory effect of CP-96,345 , a non - peptide neurokinin-1 - receptor antagonist , on neurogenic responses of guinea - pig isolated airway smooth muscle . - pubmed
The actions of CP-96,345 , a non - peptide neurokinin-1 receptor antagonist , on the responses evoked by electrical - field stimulation or by acetylcholine and substance P in guinea - pig tracheal and bronchial muscle strips were examined . - pubmed
Submaximal contractions of tracheal and bronchial muscles evoked by exogenous substance P ( 0.1 - 3 microM ) were partly inhibited by CP-96,345 ( 1 - 10 microM ) , but acetylcholine - induced contractions were hardly inhibited . - pubmed
Stimulation by galactose of protein synthesis in the presence of the heme precursor 5 - aminolevulinic acid peaked 72 h after addition to the inducer to yield 108 nmol membrane - bound P-450 2B5 per liter of culture medium . - pubmed
The recombinant enzyme was purified to near homogeneity by a two - column procedure involving chromatography on DE-52 cellulose and hydroxylapatite . - pubmed
The hemoprotein was isolated mainly in the low - spin iron configuration and exhibited a reduced CO - difference spectrum with a Soret band at 451 nm . - pubmed
Second - derivative spectral analysis in the middle - UV region revealed that type I binding of 4 - nitroanisole to ferric P-450 2B5 abolished absorption bands ascribable to tyrosine residues within the polypeptide chain . - pubmed
Pseudo - first - order rates of NADPH - driven reduction of the pigment were lower when reconstituted with NADPH - cytochrome P-450 reductase than with the mitochondrial adrenodoxin / NADPH - adrenodoxin reductase redox couple . - pubmed
The enzyme was catalytically active toward 4 - nitroanisole and androstenedione ; metabolic rates were enhanced to different extents by the presence of cytochrome b5 . - pubmed
The recombinant hemoprotein did not catalyze bioactivation of 4 -( N - methyl - N - nitrosamino )- 1 -( 3 - pyridyl )- 1 - butanone , a potent pulmonary carcinogen . - pubmed
A KEKE motif , consisting of a very hydrophilic domain rich in ' alternating ' lysine ( positive ) and glutamate ( negative ) residues , is present in the C - terminus of p40 . - pubmed
Calcium - dependent inactivation of RNA polymerase III transcription . - pubmed
We have found that these extracts no longer support RNA polymerase III transcription in response to a brief incubation in the presence of Ca2 + . - pubmed
Fractionation of extracts was used to show that transcription factors ( TF ) IIIC and , to a lesser extent , TFIIIB , but not RNA polymerase III , were targets of the Ca ( 2 +)-dependent inactivation process . - pubmed
An additional component ( not present in fractionated TFIIIC or TFIIIB ) was required for the Ca ( 2 +)-dependent destruction of transcription factor activity . - pubmed
The Ca ( 2 +)-dependent inactivation process was blocked by protease inhibitors that inhibit known Ca ( 2 +)-dependent proteases called calpains . - pubmed
The common use of Ca2 + as a second messenger and the widespread distribution of calpains suggest that the proteolytic degradation of transcription factors may be a general mechanism for the regulation of gene expression . - pubmed
whether the variability in manifestations is due to variable expressions of steroid 5 alpha - reductase 1 or to effects of testosterone itself . - pubmed
To evaluate further the possible differential expression of bFGF in melanocytic lesions , we examined 110 formalin - fixed , paraffin - embedded metastatic and primary invasive melanomas , melanomas in situ , nevi with architectural disorder and cytological atypia , and ordinary benign melanocyte nevi by nucleic acid in situ hybridization . - pubmed
In addition , defensin increased the rate of monocyte aggregation induced by arachidonic acid or phorbol myristate acetate . - pubmed
Oxidation of MA was completely inhibited by 0.1 - 1 mM semicarbazide , without being affected by the monoamine oxidase ( MAO ) inhibitor , pargyline ( 1 mM ) , indicating that MA is metabolized by semicarbazide - sensitive amine oxidase ( SSAO ) and not by MAO . - pubmed
These results are similar to previous findings that SSAO in vascular smooth muscle is able to metabolize aliphatic amines such as MA , and furthermore suggest that SSAO may play a role in adipose tissue function and/or maturation . - pubmed
The localization of riboflavin sulfates in deficient roots is similar , but not identical , to that of high iron reductase activity . - pubmed
We have previously shown that the high - affinity binding of substance P ( SP ) to its receptor is dependent on an interaction with a PTX - insensitive G protein . - pubmed
Photoincorporation of this SP derivative was susceptible to guanine nucleotide inhibition , indicating that the receptor was coupled to its G protein during labeling . - pubmed
Activation of nuclear IRF-1 GAS - binding activity by sIg was inhibited by the tyrosine kinase inhibitor , herbimycin A , and binding activity was eliminated by tyrosine phosphatase treatment . - pubmed
This heretofore described cytokine signaling pathway may play a role in bringing about Ag - specific proliferative and differentiative responses . - pubmed
However , a 28 % increase in plasma high density lipoprotein ( HDL)-cholesterol ( P & lt ; .01 ) was noted during the initial 12 - week training period , which regressed during the washout period , and was not replicated during the second 12 - week exercise period . - pubmed
A purine / pyrimidine mirror repeat element ( M - PMR3 ) in the MUC1 promoter has been shown to form H - DNA under in vitro conditions . - pubmed
TS1 , the CBD , and an IAP agonist peptide ( 4N1 K ) from the CBD of TS1 activate the platelet integrin alphaIIbbeta3 , resulting in platelet spreading on immobilized fibrinogen , stimulation of platelet aggregation , and enhanced tyrosine phosphorylation of focal adhesion kinase . - pubmed
Corticosterone levels were positively correlated with fentanyl SA . - pubmed
Predictability of the stressor also had a greater effect on maintenance of fentanyl SA than it did on relapse to fentanyl SA . - pubmed
Annexin V binding to phosphatidylserine was evaluated by flow cytometry to examine apoptosis in different lymphocyte subsets of peripheral blood mononuclear cells after a 24 h in vitro culture period . - pubmed
The antibody binding capacity ( ABC ) of lymphocyte membrane antigens was determined with CD3 - fluorescein isothiocyanate ( FITC ) , CD45RA - FITC , CD4 - phycoerythrin ( PE ) , CD8 - PE , CD56 - PE , and CD20 - PE in viable and apoptotic cells . - pubmed
In the apoptotic cells a decrease of ABC was found for all antigens , except for CD20 . - pubmed
The results showed that silicone gel # 3 ( McGhan lot # S0400488 ) produced the highest mean anti - OVA antibody titer followed by silicone gel # 1 ( DC lot # HH019581 ) and silicone gel # 2 ( McGhan lot # DP9339 ) . - pubmed
However , because EGTA does not inhibit splicing , the involvement of CaMK II itself in splicing is unlikely ; rather , a protein similar to CaMK II may be involved in spliceosome assembly and splicing . - pubmed
Cytokine and prostaglandin production by amnion cells in response to the addition of different bacteria . - pubmed
Samples for quantification of interleukin-1 beta , interleukin-6 , interleukin-8 , tumor necrosis factor - alpha , and prostaglandin E2 were collected at 6 , 12 , 24 , and 48 hours . - pubmed
coli enhanced the secretion of interleukin-8 and also induced an transient increase of prostaglandin E2 in a dose - dependent manner . - pubmed
aureus did not cause an increase in either interleukin-6 , interleukin-8 , or prostaglandin E2 . - pubmed
However , the cytotoxic activity of SBA was only slightly modified by iron , cysteine analog or catalase , in contrast to ascorbate , which was highly sensitive to all these agents . - pubmed
Therefore , the ability of one such anionic phospholipid , phosphatidic acid ( PA ) , to enhance the activity of this enzyme has been investigated in detail . - pubmed
The enhancement of hydrolysis in the presence of PA could also be achieved by prior treatment of pure PC vesicles with PLD , an effect that was dependent on the concentration of PLD and the duration of exposure to this enzyme . - pubmed
BQ-123 is extensively used as an antagonist at endothelin ( ET ) receptors , having selectivity at the ET ( A ) receptor subtype . - pubmed
In contrast , the endothelin receptor antagonists , BQ-788 , PD155080 and PD145065 ( 1 - 10 microM ) did not affect the L - type Ca2 + current . - pubmed
The mechanism of action of BQ-123 , therefore , is not confined to ET receptor antagonism . - pubmed
Three major players have been presented over the past years : ( 1 ) an inward - directed pump specific for phosphatidylserine and phosphatidylethanolamine , known as aminophospholipid translocase ; ( 2 ) an outward - directed pump referred to as ' floppase ' with little selectivity for the polar headgroup of the phospholipid , but whose actual participation in transport of endogenous lipids has not been well established ; and ( 3 ) a lipid scramblase , which facilitates bi - directional migration across the bilayer of all phospholipid classes , independent of the polar headgroup . - pubmed
Whereas a concerted action of aminophospholipid translocase and floppase could , in principle , account for the maintenance of lipid asymmetry in quiescent cells , activation of the scramblase and concomitant inhibition of the aminophospholipid translocase causes a collapse of lipid asymmetry , manifested by exposure of phosphatidylserine on the cell surface . - pubmed
Finally , phosphatidylserine exposure during apoptosis will be briefly discussed in relation to inhibition of translocase and simultaneous activation of scramblase . - pubmed
Phox2a is not only necessary but also sufficient to induce Phox2b+ dopamine - beta - hydroxylase+ and tyrosine hydroxylase+ NA neurons in ectopic locations . - pubmed
Inhibition of NHE was achieved by hypotonic stress and by using the unspecific NHE inhibitor , amiloride , or the specific NHE 1 inhibitor , cariporide ( HOE 642 ) . - pubmed
Hypotonic stress inhibited basal bile flow by 46 % and prevented secretin stimulation of bile flow by reducing biliary bicarbonate output by 50 % . - pubmed
Secretin increased bile flow from 3.7 + /- 0.8 microL / min / g to 4.78 microL / min / g ( P & lt;.01 ) ; subsequent exposure to hypotonic stress decreased secretin - stimulated bile flow by 35 % and biliary bicarbonate secretion by approximately 50 % . - pubmed
Inhibition of NHE by amiloride or cariporide resulted in a similar reduction of secretin - stimulated bile flow and bicarbonate secretion . - pubmed
In contrast , hypotonic exposure inhibited Cl (- ) , HCO ( 3 )(- ) exchange activity in isolated BECs only during basal conditions but incompletely after secretin stimulation . - pubmed
Properties of an insulin receptor with an IGF-1 receptor loop exchange in the cysteine - rich region . - pubmed
Use of rifabutin with protease inhibitors for human immunodeficiency virus - infected patients with tuberculosis . - pubmed
RESULTS : Niacin use significantly increased HDL - C by 29 % and 29 % and decreased triglycerides by 23 % and 28 % and low - density lipoprotein cholesterol ( LDL - C ) by 8 % and 9 % , respectively , in participants with and without diabetes ( P<.001 for niacin vs placebo for all ) . - pubmed
Corresponding changes in participants receiving placebo were increases of 0 % and 2 % in HDL - C and increases of 7 % and 0 % in triglycerides , and increases of 1 % and 1 % in LDL - C. Glucose levels were modestly increased by niacin ( 8.7 and 6.3 mg / dL [ 0.4 and 0.3 mmol / L ] ; P = .04 and P<.001 ) in participants with and without diabetes , respectively . - pubmed
Levels of HbA(1c ) were unchanged from baseline to follow - up in participants with diabetes treated with niacin . - pubmed
Alleles of glpR associated with the same GlpR(- ) phenotype produce substitutions in different conserved portions of the glycerol 3 - phosphate repressor which are not part of the helix - turn - helix motif . - pubmed
Reaction of nitric oxide with synthetic hemoprotein , human serum albumin incorporating tetraphenylporphinatoiron ( II ) derivatives . - pubmed
The reaction of nitric oxide ( NO ) with a synthetic hemoprotein , the recombinant human serum albumin ( rHSA ) incorporating eight tetraphenylporphinatoiron ( II ) derivatives bearing a covalently linked axial base ( FeP ) [ rHSA - FeP ] , has been investigated . - pubmed
ESR spectroscopy revealed that the incorporated FePs in the albumin formed six - coordinate nitrosyl complexes ; the proximal imidazole moiety does not dissociate from the central iron when NO binds to the trans side . - pubmed
Since NO - association is diffusion controlled , incorporation of the synthetic heme into the albumin matrix appears to restrict the NO access to the central iron ( II ) . - pubmed
This has led to a growing consensus that plasma total and low - density lipoprotein cholesterol ( LDL - C ) should be reduced to target levels that have been shown in population studies to be associated with low rates of coronary heart disease ( CHD ) . - pubmed
The aim of this study is to clarify whether the activation of a Na (+)/ H (+ ) exchanger ( NHE ) is tightly concerned with neuronal and glial cell injury induced by ischemia using a selective NHE inhibitor , SM-20220 ( N -( aminoiminomethyl )- 1 - methyl-1H - indole-2 - carboxamide methanesulfonate ) . - pubmed
SM-20220 significantly reduced LDH release in both cells in a concentration - dependent manner , and this effect was statistically significant at concentrations of more than 10(-8 ) mol / l for neurons and 10(-7 ) mol / l for glia . - pubmed
CONCLUSIONS : These data indicate that delta9 - THC selectively impairs performance of a working memory task through a CB , receptor mechanism of action and that these memory disruptions are more sensitive than other pharmacological effects of delta9 - THC . - pubmed
The As2O3 - treated cells were examined for glutathione ( GSH ) level and activity of catalase ( CAT ) , superoxide dismutase ( SOD ) , glutathione S - transferase ( GST ) and glutathione peroxidase ( GPX ) . - pubmed
However , activity of GPX , GST and SOD did not significantly correlate with their resistance to As2O3 - induced apoptosis . - pubmed
CONCLUSION : Intracellular GSH level and/or catalase activity are important factors to determine sensitivity of malignant hematopoietic cells to As2O3 - induced apoptosis . - pubmed
In the concentration range 0.5 to 2 mM , ( + /-)-isoflurane inhibited ( 45)Ca ( 2 + ) fluxes and functionally modulated the effects of the Ca ( 2 + ) channel antagonist nifedipine ( 1 - 10 microM ) . - pubmed
Isoflurane - induced inhibition of ( 45 ) Ca ( 2 + ) fluxes was not significantly affected by pretreatment with either pertussis toxin ( 5 microg / ml ) or phorbol 12 - myristate 13 - acetate ( 50 nM ) . - pubmed
In conclusion , results indicate that isoflurane , independent of intracellular Ca ( 2 + ) levels , nonstereospecifically inhibits the function of voltage - dependent Ca ( 2 + ) channels and this effect is mediated through multiple binding sites . - pubmed
This selective induction of apoptosis , which we detect by FACS analysis of intracellular HIV / p24 and concomitant surface and apoptosis marker expression , is abrogated by the glutathione precursor , N - acetyl - l - cysteine . - pubmed
Enhancement of cytarabine sensitivity in squamous cell carcinoma cell line transfected with deoxycytidine kinase . - pubmed
The reason for this is thought to be that in cell lines of solid tumors the expression of cytidine deaminase , an enzyme that degrades cytarabine , is high , whereas the expression of deoxycytidine kinase ( dCK ) , which phosphorylates cytarabine ( a prodrug ) , is weak . - pubmed
CONCLUSION : The dCK - cytarabine system may be a useful approach for gene therapy of squamous cell carcinomas of the head and neck . - pubmed
X - ray single - crystal analyses were carried out for [ Co ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )]( n ) ( ) ( 1 ) , which crystallizes in the triclinic system , space group P1 macro , with a = 6.477(1 ) A , b = 7.130(1 ) A , c = 9.826(2 ) A , alpha = 108.9(1 ) degrees , beta = 93.97(3 ) degrees , gamma = 98.82(3 ) degrees , and Z = 2 ; for [ Ni ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )( 2 )]( n ) ( ) ( 2 ) , in the triclinic system , space group P1 macro , a = 6.425(1 ) A , b = 8.115(2 ) A , c = 10.146(2 ) A , alpha = 113.09(3)(o ) , beta = 99.64(3)(o ) , gamma = 98.90(3)(o ) , and Z = 2 ; and for [ Zn ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )]( n ) ( ) ( 3 ) , in the monoclinic system , space group P2(1)/n , a = 9.044(1 ) A , b = 8.264(1 ) A , c = 11.646(1 ) A , beta = 100.77(1 ) degrees , and Z = 4 . - pubmed
None of the endothelial cell markers ( thrombomodulin , P - selectin , E - selectin , intracellular adhesion molecule , vascular cell adhesion molecule ) , thrombin and plasmin generation markers ( thrombin - antithrombin complexes , prothrombin fragments 1 + 2 , plasmin - antiplasmin complexes , D - dimers ) , protein C , protein Z , vascular endothelial growth factor , and TAFI concentration and activity were directly correlated with the level of proteinuria , albumin , cholesterol , triglycerides or creatinine , except significant positive correlations between TAFI activity and serum creatinine , E - selectin and albumin as well as negative correlations between plasmin - antiplasmin complexes and proteinuria . - pubmed
Therapeutic doses of UH are monitored by the classical coagulation assays , activated partial thromboplastin time ( aPTT ) and thrombin time ( TT ) , but also by automated chromogenic tests of anti - factor Xa activity . - pubmed
Phenotype , cytotoxic activity and cytokine production of these clones were analyzed by flow cytometry , 51Cr release assay and ELISA , respectively . - pubmed
The expression profiles of the AHP CEI12 at different IPTG concentrations and different inducing times demonstrated no significant differences by SDS - PAGE analysis . - pubmed
OBJECTIVE : A prospective double - blind randomized study undertaken to assess the effect of postoperative prophylactic " renal - dose " dopamine on post - coronary artery bypass grafting surgery 's clinical outcome . - pubmed
The mean pH was higher in group D at 8 hours after operation ( 7.38 + /- 0.2 vs 7.36 + /- 0.3 , p = NS ) , due to higher bicarbonate levels ( 23 + /- 2 mmol / l vs 21 + /- 2 , p = 0.49 ) . - pubmed
The identification of five coumarins and eleven daucane derivatives by HPLC - diode array detection ( HPLC - DAD ) and HPLC - MS is described . - pubmed
CONCLUSIONS : Both physician and patient - rated scales indicate that fluvoxamine CR is effective and safe for the treatment of generalized social anxiety disorder . - pubmed
Less frequent are patients with an obstruction in the outflow tract of the heart or disorders in binding oxygen to hemoglobin . - pubmed
3 - Hydroxy-3 - methylglutaryl coenzyme A reductase inhibitors ( statins ) are potent inhibitors of cholesterol biosynthesis . - pubmed
With respect to these effects , statins augment the expression of bone morphogenetic protein-2 , a potent simulator of osteoblast differentiation and its activity , and promote mineralization by cultured osteoblasts , indicating that statins have an anabolic effect on bone . - pubmed
In the succinate experiments the mitochondria were contracted in buffered KCl layered over sucrose in centrifuge tubes and centrifuged down through distinct wash , reactive and isotope exchange layers . - pubmed
The stability of CaX approximately I must be low for the mitochondria swell very rapidly upon exhaustion of NADH or blocking of succinate oxidation by cyanide . - pubmed
The subjects were initially screened by an enzyme immunoassay whereas Western blot , INNO - LIA HTLV , polymerase chain reaction and nucleotide sequencing confirmed the infection . - pubmed
We report herein the results of fully atomistic molecular dynamics ( MD ) simulations of a series of Gemini surfactants : CsH2s - alpha , omega - bis ( C12H25N +( CH3 ) 2Cl - ) , at the air / water interface with s = 3 , 4 , 6 , 12 , 14 , and 16 , at values of the initial surface area per surfactant AS = 70 A2 , 77 A2 , 95 A2 , 151 A2 , 133 A2 , and 103 A2 , respectively . - pubmed
The Rho family of GTPases regulates many aspects of cellular behavior through alterations to the actin cytoskeleton , acting as molecular switches cycling between the active , GTP - bound and the inactive , GDP - bound conformations . - pubmed
RhoE protein levels increase upon exposure to UVB , and ablation of RhoE induction through small interfering RNA resulted in a significant increase in apoptosis and a reduction in the levels of the pro - survival targets p21 , Cox-2 , and cyclin D1 , as well as an increase of reactive oxygen species levels when compared with control cells . - pubmed
After the GST - HER2 ECD inclusion bodies were solubilized with denaturation buffer containing 8 M urea , an approach was then used to optimize refolding parameters . - pubmed
Comparison of apolipoprotein B and non - high - density lipoprotein cholesterol for identifying coronary artery disease risk based on receiver operating curve analysis . - pubmed
Whether or non - high - density lipoprotein cholesterol is equivalent to apolipoprotein B ( apo B ) for screening remains controversial . - pubmed
We found that post - pMCAO injections with HDAC inhibitors , valproic acid ( VPA ) , sodium butyrate ( SB ) , or trichostatin A ( TSA ) , decreased brain infarct volume . - pubmed
The reduction in levels of acetylated histone H3 in the ischemic brain was prevented by treatment with VPA , SB , or TSA . - pubmed
Moreover , injections with HDAC inhibitors superinduced heat - shock protein 70 and blocked pMCAO - induced down - regulation of phospho - Akt , as well as ischemia - elicited up - regulation of p53 , inducible nitric oxide synthase , and cyclooxygenase-2 . - pubmed
After 3 months ' atorvastatin therapy , serum levels of total cholesterol ( TC ) , low - density lipoprotein cholesterol ( LDL - C ) and coenzyme Q10 ( 0.43 + /- 0.23 to 0.16 + /- 0.10 microg / mL ) were all significantly reduced ( all p<0.001 ) . - pubmed
On day 2 after the last atorvastatin , the coenzyme Q10 level was significantly elevated ( 0.37 + /- 0.16 microg / mL ) and maintained the same levels on day 3 ( 0.39 + /- 0.18 microg / mL ) compared with those on month 3 ( both p< ; 0.001 ) , while TC and LDL - C did not significantly change within the same 3 days . - pubmed
These prostanoids and their deuterium derivatives , which were used as internal standards , were subjected to solid - phase extraction using Empore C18 HD disk cartridges and analyzed in the selected reaction - monitoring mode . - pubmed
In contrast , none of the PPAR - gamma agonists tested ( 0.1 , 1 and 10 microM ) altered the basal and the insulin ( 0.1 microM)-induced [ ( 3 ) H ]- glucose uptake into adipocytes of both species . - pubmed
Novelty seeking was not associated with differences in 5HIAA , HVA , MHPG , or CRF , but correlated with differences in object exploration observed in a different test situation at an earlier age . - pubmed
PURPOSE : Ispinesib , a kinesin spindle protein inhibitor , blocks assembly of a functional mitotic spindle , leading to G2 / M arrest . - pubmed
Docetaxel promotes tubulin assembly into microtubules while inhibiting microtubule de - polymerization leading to mitotic arrest . - pubmed
The goal was to fit a Bayesian population PK / PD model to characterize the relationship between the ispinesib / docetaxel combination and absolute neutrophil counts ( ANC ) . - pubmed
The PK properties of ispinesib and docetaxel , and the relationship of PK with ANC were investigated using nonlinear mixed - effects models and Bayesian methods . - pubmed
This ANC model has been used previously for ispinesib given as monotherapy , and for other chemotherapeutic drugs in the literature . - pubmed
An electrophysiological analysis of heterologous expression system exhibited that R468Q - CACNB4 showed greater Ba ( 2 + ) current density compared with the wild - type CACNB4 . - pubmed
The greater Ca(v)2.1 currents caused by the R468Q - CACNB4 mutation may increase the neurotransmitter release in the excitatory neurons under the condition of insufficient inhibitory neurons caused primarily by the SCN1A mutation . - pubmed
Previously , a series of structurally related novel 7 - membered iminocyclitols were synthesised and found to be inhibitors of alpha - mannosidase using in vitro assays . - pubmed
The present study aimed to delineate alpha - mannosidases hydrolytic pathways in azepane inhibitor treated cells by the analysis of free oligosaccharides ( FOS ) as markers of endoplasmic reticulum ( ER ) , Golgi , lysosomal and cytosolic alpha - mannosidase activities . - pubmed
Specific ER - mannosidase inhibition by kifunensine also reveals significant non - proteasomal degradation following FOS analysis and appears to be cell line dependent . - pubmed
After induction therapy , remission was maintained with low - dose prednisone and azathioprine for 4.5 years despite high levels of anti - MPO antibodies ( & gt;100 U / mL ) . - pubmed
During the postadolescent period ( 10 - 14 weeks postnatal ) , immunohistochemical experiments were carried out to investigate c - Fos expression following the administration of R -(+)- 8 - hydroxy-2 -( di - n - propylamino ) tetralin ( 8 - OH - DPAT ) , a 5 - HT ( 1A ) receptor agonist . - pubmed
In the 3wFS group , the 8 - OH - DPAT - induced c - Fos expression in the medial prefrontal cortex was significantly attenuated compared to that in the non - FS control group . - pubmed
A recent report in the New England Journal of Medicine on the early clinical evaluation of Olaparib in cancers lacking the BRCA1 or BRCA2 genes exemplifies this promising new trend . - pubmed
KIT alterations have been identified in melanoma and treatment with imatinib has met with some success . - pubmed
The test - retest reliability of the SDS was found to be very good ( the ICC for all the centres was 0.76 ; overall kappa value for SDS questions was 0.58 ; one - third of SDS questions had a kappa value of 0.60 or higher ) . - pubmed
Her full blood count , urea , electrolytes , uric acid , calcium , phosphate , alkaline phosphatase , and thyroid function levels were all within normal ranges , and her serum was negative for rheumatoid factor . - pubmed
Carbonyl reductase was immobilized on silica with an optimized protein loading of 4 mg / g support . - pubmed
Parotid constraints were a mean dose to either parotid & lt ; 26 Gy ( PP1 ) , 50 % of either parotid & lt ; 30 Gy ( PP2 ) , or 20 cc of total parotid & lt ; 20 Gy ( PP3 ) . - pubmed
PP2 ( parotid constraint of 30 Gy to less than 50 % of one parotid ) was the easiest to achieve ( group I , II , and III : 82 % , 76 % , and 78 % , respectively ) . - pubmed
Among these cycle - arrested cells , the proportion of cells in M phase was down - regulated after OTA treatment by the mitotic index and the level of phospho - histone H3 . - pubmed
The cyclinB1 - Cdc2 complex was reduced and the expression of Cdc25C , Cdc2 and cyclinB1 were significantly decreased by OTA treatment both at protein and mRNA level , respectively . - pubmed
In conclusion , cell apoptosis and G(2 ) phase arrest mediated by Cdc25C , Cdc2 and cyclinB1 may be the initiating event in the gastric toxicity of OTA . - pubmed
However , the substitution mutation mlt-10(mg364 ) , which disrupts the proline - rich repeats , causes the most severe phenotype . - pubmed
The neurological impairment was regressive within 6 months after intravenous immunoglobulin treatment followed by intravenous then oral corticosteroid administration . - pubmed
High - dose clopidogrel significantly reduced OTR from 285 + - 47 P2Y(12 ) reaction units ( PRU ) to 220 + - 91 PRU ( p & lt ; 0.001 ) . - pubmed
The structures of the self - assembled monolayers of various 4 - alkoxybenzoic acids physisorbed at the liquid - solid interface were established by employing scanning tunnelling microscopy ( STM ) . - pubmed
This solvent - induced convergence of supramolecular patterns is attained by means of co - crystallisation of octanoic acid molecules in the 2D crystal lattice , which is evident from high - resolution STM images . - pubmed
Analyzing together all the data , taking into account the plasma melatonin concentration versus the expression of adhesion molecules or iNOS we detected a significant inverse correlation . - pubmed
Caspase inhibitors altered the effects of regression induced by peroxide or vitamin C. - pubmed
At the same time , caspase inhibitors had little effect on reactive nitrogen species that are also associated with regression . - pubmed
Lowered expression of heparan sulfate / heparin biosynthesis enzyme N - deacetylase / n - sulfotransferase 1 results in increased sulfation of mast cell heparin . - pubmed
Surprisingly , the relative amount of heparin produced in NDST1(+/- ) and NDST1(-/- ) MCs is higher ( 30 % ) than in control MCs where 95 % of the ( 35 ) S - labeled glycosaminoglycans produced is chondroitin sulfate . - pubmed
Lowered expression of NDST1 also results in a higher sulfate content of the heparin synthesized and is accompanied by increased levels of stored MC proteases . - pubmed
Carbonic anhydrase inhibitors : in vitro inhibition of alpha isoforms ( hCA I , hCA II , bCA III , hCA IV ) by flavonoids . - pubmed
A series of flavonoids , such as quercetin , catechin , apigenin , luteolin , morin , were investigated for their inhibitory effects against the metalloenzyme carbonic anhydrase ( CA ) . - pubmed
The flavonoids inhibited hCA I with K(I)-s in the range of 2.2 - 12.8 muM , hCA II with K(I)-s in the range of 0.74 - 6.2 muM , bCA III with K(I)-s in the range of 2.2 - 21.3 muM , and hCA IV with inhibition constants in the range of 4.4 - 15.7 , with an esterase assay using 4 - nitrophenyl acetate as substrate . - pubmed
( 2 ) Activation of cell - mediated immune pathways as indicated by an increased production of interferon gamma and neopterin . - pubmed
( 4 ) Lowered levels of key antioxidants , such as coenzyme Q10 , zinc , vitamin E , glutathione , and glutathione peroxidase . - pubmed
using cytokine antagonists , minocycline , Cox-2 inhibitors , statins , acetylsalicylic acid , ketamine , omega3 poly - unsaturated fatty acids , antioxidants , and neurotrophic factors . - pubmed
Pharmacological LXR activation reduces presence of SR - B1 in liver membranes contributing to LXR - mediated induction of HDL - cholesterol . - pubmed
Part of these beneficial effects may be explained by accelerated reverse cholesterol transport since both plasma high density lipoprotein ( HDL ) cholesterol and fecal neutral sterol secretion are higher upon LXR activation . - pubmed
RESULTS : HDL cholesterol was maximally 60 % increased in a time - dependent fashion due to appearance of more and larger HDL particles . - pubmed
Surprisingly , LXR activation reduced SR - B1 protein amount in hepatic membranes , suggesting that this might contribute to elevated HDL cholesterol . - pubmed
In addition , SR - B1 is not essential for LXR - induced cholesterol removal from the body . - pubmed
CONCLUSION : Induction of fecal neutral sterol secretion by T0901317 critically depends on LXRalpha but not on LXRbeta . - pubmed
LXR activation reduces SR - B1 in hepatic membranes , probably partly contributing to elevated HDL cholesterol . - pubmed
SR - B1 is not required to enhance fecal neutral sterol secretion . - pubmed
present the structure of an extracellular undecaheme cytochrome involved in the step of electron transfer to metal oxides . - pubmed
MetS in children is based on the presence of any three of the following : waist circumference & gt;= 90th percentile for age and sex ; plasma triglyceride & gt;= 1.24 mmol l(-1 ) ; plasma high - density lipoprotein - cholesterol & lt;= 1.03 mmol l(-1 ) ; systolic or diastolic blood pressure & gt;= 90th percentile for age , sex , and height ; and fasting glucose & gt;= 5.6 mmol l(-1 ) . - pubmed
Models adjusted for duration of SGA treatment , ethnicity , sex , age and use of other medications revealed a positive relationship between the MTHFR 677 T allele and diastolic blood pressure Z - scores ( P=0.001 ) and fasting plasma glucose ( P<0.05 ) in SGA - treated children . - pubmed
The aim of the study was to determine the influence of treatment with heparin on protective effect of ischemic preconditioning ( IP ) in ischemia / reperfusion - induced acute pancreatitis . - pubmed
Pretreatment with heparin or IP applied alone reduced the severity of acute pancreatitis . - pubmed
Combination of heparin plus IP reduced the protective effect of heparin or IP applied alone . - pubmed
The unsaturated C-6 ' linoleic acid ester derivative 14 that was structurally most similar to 1 and 2 , inhibited HIF-1 activation with comparable potency ( IC50 0.92 muM ) . - pubmed
The octanoyl ( 11 ) and undecanoyl ( 12 ) ester derivatives also suppressed HIF-1 activation ( IC50 values 3.1 and 0.87 muM , respectively ) . - pubmed
One surprising observation was that , while less potent , the purported cancer chemopreventive agent auraptene ( 8) was found to act as a mitochondrial poison that disrupts HIF-1 signaling in tumors . - pubmed
obtusiloba inhibit mast - cell - derived allergic inflammation , histamine , and proinflammatory cytokines . - pubmed
In summary , direct phosphorylation of the A subunit at Ser-384 by AMPK represents a novel regulatory mechanism of the V - ATPase in kidney intercalated cells . - pubmed
With sulfadimethoxine and thrombin as model analytes , we found that water resuspended AuNPs can significantly increase the sensitivity by more than 10 - fold for AuNPs based fluorescence quenching aptasensor . - pubmed
Design and synthesis of novel pyrimidone analogues as HIV-1 integrase inhibitors . - pubmed
A series of novel pyrimidone analogues have been designed and synthesized as HIV-1 integrase ( IN ) inhibitors . - pubmed
This study demonstrated that introducing a substituent in the N1 - position of the pyrimidone scaffold does not significantly influence IN inhibitory activity . - pubmed
The carboxy - terminal segment of the human LINE-1 ORF2 protein is involved in RNA binding . - pubmed
Here , we explore the nucleic acid binding properties of the 180 - amino acid carboxy - terminal segment ( CTS ) of the human L1 ORF2p in vitro . - pubmed
Finally , we report that mutations destroying the putative Zn - knuckle structure of the protein do not significantly affect the level of RNA binding and discuss the possible functional role of the CTS in L1 retrotransposition . - pubmed
BACKGROUND : A combination of inhaled corticosteroid and long - acting beta2 agonist ( ICS / LABA ) is used frequently to treat chronic obstructive pulmonary disease ( COPD ) patients . - pubmed
[ Associations of ulcerative colitis with vitamin D receptor gene polymorphisms and serum levels of 25 - hydroxyl vitamin D ] . - pubmed
OBJECTIVE : To explore the associations of ulcerative colitis ( UC ) with vitamin D receptor ( VDR ) gene polymorphisms and serum levels of 25 - hydroxyl vitamin D[25 ( OH ) D ] . - pubmed
Four single nucleotide polymorphisms of VDR ( Fok I , Bsm I , Apa I , Taq I ) were detected by mini - sequencing technique . - pubmed
By linear regression analysis , the serum levels of 25 ( OH ) D in UC patients were independently and positively correlated with hemoglobin ( beta = 0.499 , P & lt ; 0.01 ) and yet independently and negatively correlated with C - reaction protein ( beta = -0.346 , P & lt ; 0.01 ) and white blood cells ( beta = -0.291 , P = 0.002 ) . - pubmed
CONCLUSIONS : Genetic polymorphism of VDR ( Fok I , Bsm I , Apa I , Taq I ) and serum levels of 25 ( OH ) D are significantly correlated with UC . - pubmed
QYHJ upregulated the mRNA and protein level of Eph receptor - interacting B1 ( EphrinB1 ) in the cells that were expressing different levels of EphB2 , however , QYHJ did not regulate EphB2 expression . - pubmed
Mean total testosterone , LH , and FSH were 6.2 + - 1.7 nmol / L , 3.4 + - 2 and 4.7 + - 3.1 mIU / L , respectively . - pubmed
The photodegradation of methylene blue by CdS photocatalyst confined inside VLP can be significantly enhanced by the controlled deposition of gold nanoparticles on the outer shell of VLP - CdS. - pubmed
CONCLUSION : Within the limitations of this study , dimensional changes of acrylic resin specimens were influenced by the molding technique used and SR - Ivocap injection procedure exhibited higher dimensional accuracy compared to conventional molding . - pubmed
The leukotriene B4 ( LTB4 ) receptor 1 ( BLT1 ) is a high affinity receptor for LTB4 , a chemotactic and inflammatory eicosanoid . - pubmed
The LTB4 receptor 2 ( BLT2 ) was originally identified as a low affinity receptor for LTB4 , and , more recently , as a high affinity receptor for 12 - hydroxyheptadecatrienoic acid ( 12 - HHT ) . - pubmed
Consistently , knockdown of lta4h , an enzyme mediating LTB4 production , induced a phenotype similar to knockdown of blt1 . - pubmed
Histone deacetylases ( HDACs ) are enzymes that catalyze the removal of acetyl groups from the epsilon - amino groups of conserved lysine residues in the amino terminal tail of histones . - pubmed
The mechanism is poorly understood ; it is unclear whether phosphate has direct effects or effects mediated via calcification or FGF23 . - pubmed
Oxcarbazepine ( Na channel blocker ) and pregabalin ( calcium channel alpha2 - delta ligand medication ) were tried . - pubmed
The disease is characterized by type 1 hypersensitivity to fungal allergens , dramatically elevated total serum IgE , accumulation of thick eosinophil - laden mucin with non - invasive fungal hyphae within the paranasal sinuses , nasal polyposis , and sinus bony remodeling . - pubmed
The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na + K +- ATPase alpha - subunits . - pubmed
Desensitization Using Bortezomib and High - dose Immunoglobulin Increases Rate of Deceased Donor Kidney Transplantation . - pubmed
Role of Serum Follicle Stimulating Hormone , Luteinizing Hormone , Testosterone and Prolactin Levels in Azoospermic Male Partner of Subfertile Couple . - pubmed
Using standard ELISA technique , serum levels of follicle stimulating hormone ( FSH ) , luteinizing hormone ( LH ) , testosterone and prolactin were measured / assayed / estimated . - pubmed
Low testosterone level ( & lt;270ng / dl ) was observed in 11(13.6 % ) , low TSH ( & lt;0.4muIU / ml ) in 1(1.2 % ) and low prolactin ( & lt;2.5ng / dl ) in 5(6.2 % ) . - pubmed
CD melting experiments demonstrate that spiroketal does not affect the G - quadruplex ( G4 ) thermal stability . - pubmed
Compared with the cells in which RNAi of the CAS gene was achieved , the cells with simultaneously over - expressed FPS and silenced CAS showed higher triterpene contents . - pubmed
These results demonstrate that over - expression of FPS can break the rate - limiting reaction catalyzed by FPS in the triterpene saponins biosynthetic pathway ; and inhibition of CAS expression can decrease the synthesis metabolic flux of the phytosterol branch . - pubmed
The subanesthesia dose sevoflurane in 60 % oxygen may reduce sepsis - induced inflammatory responses in animals and in PBMCs , and the inhibition to the activation of the NF - kappaB pathway may contribute to this protection . - pubmed
The aim of this study was to investigate epidermal growth factor receptor ( EGFR ) mutation status in lung adenocarcinoma - associated MPEs ( LA - MPEs ) and its correlation with efficacy of EGFR tyrosine kinase inhibitor ( TKI ) therapy . - pubmed
The full - length cDNA sequence of L - LECT2 consists of a 606 - bp ORF encoding a protein of 201 amino acid residues . - pubmed
The low mGluR5 protein group ( LE ) showed increased methylation of the mGluR5 CpG island , reduced mGluR5 mRNA levels , and unaltered basal EEG theta spectral power between stress day 1 and 6 . - pubmed
In contrast , the high mGluR5 protein group ( HE ) showed reduced methylation of CpG sites , increased mGluR5 mRNA expression , and reduced basal theta spectral power on stress day 6 . - pubmed
Nitrogen - doped graphene oxide sheets ( N - GOs ) are prepared by employing N - containing polymers such as polypyrrole , polyaniline , and copolymer ( polypyrrole - polyaniline ) doped with acids such as HCl , H2SO4 , and C6H5 - SO3 - K , which are activated using different concentrations of KOH and carbonized at 650 C ; characterized using SEM , TEM , BET , TGA - DSC , XRD , and XPS ; and employed for the removal of environmental pollutant CO2 . - pubmed
Furthermore , IrM2 rapidly induces a series of mitochondria - related dysfunctional events , including the loss of mitochondrial membrane potential , cellular ATP depletion , mitochondrial respiration inhibition and reactive oxygen species ( ROS ) elevation . - pubmed
Among the ROS , superoxide anion radicals play a critical role in IrM2 - mediated cell death . - pubmed
Preparation of Complexes between Ovalbumin Nanoparticles and Retinoic Acid for Efficient Induction of Tolerogenic Dendritic Cells . - pubmed
We have proposed a simple formulation of ovalbumin nanoparticles complexed with ATRA ( OVA / RA NPs ) . - pubmed
This protocol successfully avoids the isolation , purification and storage of carcinogenic isocyanates and allows elaboration of urethanes and ureas in a one - pot process from commercially available sources . - pubmed
A mechanistic study verified that nanosilica induced autophagy via negative regulation of mammalian target of rapamycin ( mTOR ) signaling but not the Beclin-1 associated pathway . - pubmed
Additionally , production of lymphoid chemokines and cytokines associated with ectopic lymphoneogenesis and , remarkably , saliva flow and autoantibody production , were significantly affected by treatment with seletalisib . - pubmed
Interest has been expanded to areas not only limited to low - density lipoprotein cholesterol reduction but also investigating other non - lipid pathways raising cardiovascular risk , in particular inflammation associated to raised high - sensitivity C - reactive protein levels , not significantly affected by the present PCSK9 antagonists . - pubmed
In this study , chitosan ( CS ) loading well - dispersed nanoscale zero - valent iron ( NZVI / CS ) was successfully prepared via the liquid - phase reduction method . - pubmed
Characterizations of the NZVI / CS with high - resolution transmission electron microscopy and X - ray diffraction suggested that the as - prepared NZVI / CS comprised numerous dispersed Fe0 nanoparticles . - pubmed
These findings indicate that the synthesized NZVI / CS composites have potential application for the removal of U ( VI ) from the sewage water . - pubmed
Mean Urine albumin - to - creatinine ratio ( UACR ) was 0.02 , and 166 subjects showed significant albuminuria ( UACR & gt;= 0.03 ) . - pubmed
The EQE of an OPD with P3HT : DRCN5 T ( 100 : 2 , wt / wt ) as the active layer reaches 1400 % at -10 V , exhibiting the photomultiplication ( PM ) phenomenon . - pubmed
The macrosomia rate was higher in the glibenclamide - exposed group than the insulin - alone group ( 12.2 % vs 0 % , P=0.025 ) . - pubmed
The Na - K - Cl cotransporter-1 ( NKCC1 ) , by mediating the electroneutral transport of Na+ , K + , and Cl- plays an important role in cell volume regulation , epithelial transport , and the control of neuronal excitability . - pubmed
Studies using catalase mutants have revealed that the inducible and constitutive elevations of intracellular hydrogen peroxide ( H2O2 ) have different roles : only the inducible H2O2 production transduces the abscisic acid ( ABA ) signal leading stomatal closure . - pubmed
We studied H2O2 and NO mobilization in guard cells of catalase mutants . - pubmed
Abbreviations : ABA : abscisic acid ; CAT : catalase ; cGMP : cyclic guanosine monophosphate ; DAF-2DA : 4,5 - diaminofluorescein-2 diacetate ; H2DCF - DA : 2 ' , 7 '- dichlorodihydrofluorescein diacetate ; MeJA : methyljasmonate ; NOS : nitric oxide synthetase ; NR : nitrate reductase ; POX : peroxidase ; ROS : reactive oxygen species ; SNAP : S - nitroso - N - acetyl - DL - penicillamine ; SNP : sodium nitroprusside ; NOX : NADP ( H ) oxidase . - pubmed
Meta - analysis showed that the changed FMD value before and after treatment with nebivolol was not statistically different from those treated with other beta - blockers [ Mean difference=1.12 , 95 % CI ( -0.56,2.81 ) , P=0.19 ] . - pubmed
Moreover , the correlations of ACE2 with CS - induced oxidative stress - related markers , hypoxia inducible factor-1alpha ( HIF-1alpha ) , inducible nitric oxide synthase ( iNOS ) , and 4 - hydroxynonenal ( 4 - HNE ) were investigated . - pubmed
The modification of HA on Pd@Pt - GOx could block the GOx activity , catalase ( CAT)-like and peroxidase ( POD)-like activities of Pd@Pt , reduce the cytotoxicity to normal cells and organs , and effectively target CD44 - overexpressed tumors by active targeting and passive enhanced permeability and retention ( EPR ) effect . - pubmed
After endocytosis by tumor cells , the intracellular hyaluronidase ( Hyase ) could decompose the outer HA and expose Pd@Pt - GOx for the enzymatic cascade reaction . - pubmed
Meanwhile , the upregulated acidity and H2O2 content in the tumor region generated by GOx catalytic oxidation of glucose dramatically facilitated the pH - responsive POD - like activity of the Pd@Pt nanozyme , which then catalyzed degradation of the H2O2 to generate abundant highly toxic OH , thereby realizing nanozyme - mediated starving - enhanced chemodynamic cancer therapy . - pubmed
Molecular subtype - specific responses of colon cancer cells to the SMAC mimetic Birinapant . - pubmed
In this study , responses to the IAP antagonist Birinapant and oxaliplatin/5 - fluorouracil ( 5 - FU ) were investigated in 14 colon cancer cell lines , representing the consensus molecular subtypes ( CMS ) . - pubmed
We observed increased cell death during Birinapant single treatment in these co - cultures , which was abrogated by anti - TNFalpha - neutralizing antibodies . - pubmed
Collectively , our study demonstrates that IAP inhibition is a promising modulator of response to oxaliplatin/5 - FU in colorectal cancers of the CMS1 subtype , and may show promise as in the CMS2 subtype , suggesting that molecular subtyping may aid as a patient stratification tool for IAP antagonists in this disease . - pubmed
Amid those , some bind to yet untapped amino - acids in the S1 pocket . - pubmed
Cisternography and CT scanning with 111In - DTPA in evaluation of posterior fossa arachnoid cysts . - pubmed
Mechanism of action of adenosylcobalamin : hydrogen transfer in the inactivation of diol dehydratase by glycerol . - pubmed
We have investigated the kinetic characteristics of the inactivation of the adenosylcobalamin - dependent enzyme propanediol dehydratase by glycerol , ( RS )- 1,1 - dideuterioglycerol , ( R )- 1,1 - dideuterioglycerol , and perdeuterioglycerol in the presence of 1,2 - propanediol and 1,1 - dideuterio-1,2 - propanediol . - pubmed
When diol dehydratase holoenzyme is inactivated by [ 1 - 3H ] glycerol , 5 '- deoxyadenosine which is enriched in tritium by a factor of 2.1 over that in glycerol can be isolated from the reaction mixture . - pubmed
Disease specific accumulation of PrP was observed at light microscopy as amyloid plaques or as diffuse or granular staining within the neuropil , often clearly associated with individual neurons . - pubmed
Serial electron microscopical preparations were immunostained for PrP by the immunogold method . - pubmed
This suggests that amyloid fibrils are formed following the accumulation and aggregation of sub - unit proteins at the plasmalemma and , furthermore , that normal PrP may be converted to its pathological form at this site . - pubmed
Moreover , in these clones H-2Kb expression could be enhanced in the presence of Zn2 + , indicating that the metallothionein enhancer was functioning properly . - pubmed
It is concluded that those with frequent occupational exposure to M tuberculosis have larger numbers of circulating T cells reactive with mycobacterial antigens , so that the development of the skin test response to tuberculin is less dependent on " by - stander " cell infiltration to mediate the delayed hypersensitivity reaction than the reactions in those with less intense and less frequent natural exposure . - pubmed
These findings were consistent with the hypothesis that metrizamide is a competitive inhibitor of human brain hexokinase . - pubmed
The maximum change in polymorphonuclear leukocytes ( PMNs ) was detected 6 h after O3 , and the maximum increase in BAL protein occurred 24 h postexposure . - pubmed
The phenotypes of the C57BL/6J ( B6 , termed susceptible ) and C3H / HeJ ( C3 , termed resistant ) strains were easily distinguished by the magnitude of their inflammatory responses to O3 . - pubmed
The treatment with the 5 mg dose had minor and inconsistent effect on the motility , hyaluronidase and acrosin activity , and the fertilizing ability of spermatozoa in addition to the blood level of testosterone . - pubmed
The role of histamine in cardiac anaphylaxis ; characterization of histaminergic H1 - and H2 - receptor effects . - pubmed
It is concluded that histamine might be an important mediator of these effects , since in the presence of H2 - receptor blockade with cimetidine ( 6.2 x 10(-7 ) moles / min ) , the positive inotropic and chronotropic effects were completely antagonized . - pubmed
The O2 reducing ability of the organism is due to the presence in the cells of a particle - bound NADH oxidase , a soluble NADPH oxidase and a soluble pyruvate oxidase . - pubmed
The two pyridine nucleotide oxidases reduce O2 to H2O2 , the pyruvate oxidase reduces O2 to H2O. - pubmed
Kinetic studies with cell - free extracts showed that the pyruvate oxidase had a considerably greater affinity ( smaller Km ) for O2 and capacity ( higher Vmax ) for O2 reduction than the two other oxidases . - pubmed
It is postulated that the pyruvate oxidase acts as a scavenger for O2 , leading to the non - toxic reduction product H2O , and thus functions as a defense mechanism against oxygen toxicity when the organism is exposed to aerobic condition . - pubmed
The effect of various drugs affecting the dopaminergic system in the brain were also investigated to clarify the relationship between NT and dopamine . - pubmed
Intraperitoneal(ip ) bolus administration of L - DOPA caused the reduction in NTLI concentration from 9.2 + /- 1.0 ng / g wet wt to 5.3 + /- 2.1 ng / g wet wt and Bmax of NT receptor from 19.7 + /- 3.1 fmol / mg protein to 13.9 + /- 2.6 fmol / mg protein with no change in Kd . - pubmed
Intracerebroventricular administration of dopamine also induced a significant reduction of NTLI concentration and Bmax of NT receptor with no change in Kd . - pubmed
Inversely , ip bolus administration of alpha - methyldopa evoked an increase in NTLI concentration from 6.9 + /- 0.9 ng / g wet wt to 12.0 + /- 3.0 ng / g wet wt and that in Bmax of NT receptor from 15.9 + /- 2.9 fmol / mg protein to 24.2 + /- 4.6 fmol / mg protein . - pubmed
Serial administration of haloperidol evoked an increase in Bmax of NT receptor from 16.9 + /- 1.5 fmol / mg protein to 20.5 + /- 1.9 fmol / mg protein with no changes in Kd and NTLI concentration . - pubmed
Like the engrailed gene , the invected gene can encode a protein of approximately 60 kD that contains a homeo box near its carboxyl terminus ; indeed , a sequence of 117 amino acids in the carboxy - terminal region of both proteins is almost identical . - pubmed
The induction of cytochrome P-450 by isosafrole and related methylenedioxyphenyl compounds . - pubmed
Isosafrole , piperonyl butoxide , and 5 - t - butyl-1,3 - benzodioxole were unable to displace 2,3,7,8 - tetrachlorodibenzo - p - dioxin or 3 - methylcholanthrene from either the Ah receptor or the 3 - 4S binding peak , in vitro . - pubmed
injection ; whereas , isosafrole caused a 2 - fold enhancement of the Ah receptor after 24 h. This increase in the Ah receptor binding following isosafrole treatment may be due to induction . - pubmed
Both in vivo and in vitro data appear to demonstrate that there is no direct role for the Ah receptor or the 3 - 4S protein in the regulation of cytochrome P-450 by methylenedioxyphenyl compounds . - pubmed
We examined the production of oxygen radicals by synovial fluid PMNs ( SF - PMNs ) and peripheral blood PMNs ( PB - PMNs ) by measuring chemiluminescence ( CL ) as well as superoxide anion ( O2- ) release . - pubmed
Increased spontaneous CL in the presence of luminol and increased CL in response to phorbol myristate acetate ( PMA ) was observed in SF - PMNs when compared to PB - PMNs . - pubmed
When zymosan was used as the stimulus in the absence of luminol , a slightly lower CL response was observed in SF - PMNs as compared to PB - PMNs . - pubmed
When O2- release from normal PB - PMNs pretreated with 10 % synovial fluid was compared to untreated controls , enhancement of spontaneous O2- release was observed . - pubmed
Increased spontaneous and PMA - stimulated release of myeloperoxidase ( MPO ) was also observed in normal PB - PMNs pretreated with synovial fluid . - pubmed
These findings may explain the increased luminol - dependent CL since this type of CL requires the presence of MPO . - pubmed
Our findings suggest that the enhanced chemiluminescence observed in normal PMNs treated with synovial fluids may be related to increases in spontaneous O2- generation and myeloperoxidase release . - pubmed
A tumor promoter , 12 - O - tetradecanoylphorbol-13 - acetate ( TPA ) also augmented anchorage - independent growth of ras - transformed cells and induced morphological changes in monolayer cultures without altering the expression of the ras gene or phosphorylation of the p21ras protein . - pubmed
Although the total percentages of PUFA in the two phosphoglycerides were not altered , compared with controls the ratio of PUFA of the ( n-3 ) series to those of the ( n-6 ) series was very significantly elevated in Down ' s syndrome , from 0.32 to 0.55 in EPG and from 0.60 to 0.97 in SPG . - pubmed
In particular , docosahexaenoyl , 22:6(n-3 ) , groups were uniformly increased in Down ' s syndrome compared with controls by 54 % and 33 % in EPG and SPG , respectively , while arachidonoyl , 20:4(n-6 ) , groups were decreased by 16 % and 30 % , respectively . - pubmed
A control test with a single oral dose of 2.5 mg bromocriptine was also performed ; serum PRL and GH levels were measured at the same time intervals for 2 days . - pubmed
Cabergoline induced a marked fall in serum PRL which began within 3 h and continued for 7 days . - pubmed
31P spectra were obtained using a surface coil placed into the surgically created pouch and using routine NMR pulsing techniques . - pubmed
Sedimentation analysis of cell sap in sucrose density gradients shows a shift of prolyl - tRNA synthetase activity toward the high - Mr form in regenerating liver . - pubmed
Under artificial photoperiods ganglionectomy suppressed the stimulatory role of long days and the inhibitory role of short days on prolactin secretion , and consequently on progesterone secretion and spring moult . - pubmed
However , in animals kept outdoors , prolactin and progesterone secretion and spring moult were not changed by ganglionectomy . - pubmed
[ Reduction of alpha-2 - globulins following administration of 1 - methyl - delta-1 - androstenolone enonthate ] . - pubmed
The location and identity of different autonomic ( sympathetic and parasympathetic ) and sensory nerves was further studied by monoamine histofluorescence , acetylcholinesterase histochemistry , immunohistochemistry for substance P and neurofilament , and Linder 's silver staining . - pubmed
Ca2 + -dependence of thrombin - induced aggregation and MDA formation , and Ionophore A23187 - induced aggregation of the platelets from SHRSP at a hypertensive age(16 - weeks ) was similar to that of the aggregation of platelets from age - matched WKY . - pubmed
The enhancement by Ca2 + of both thrombin- and Ionophore A23187- induced aggregation , however , was markedly less in SHRSP than in WKY , whereas their MDA formation was equally enhanced by Ca2 + . - pubmed
The magnitude of phospholipid(PI , PC , PE ) degradation , and MDA formation IN SHRSP at early- and late- hypertensive ages(11- and 17 - weeks ) were either the same as or greater than that of age - matched WKY . - pubmed
In addition , thrombin - induced thromboxane B2 formation in SHRSP platelets was similar to that in WKY in the concentration range of 0.22 - 0.44 U / ml , and became significantly higher at 0.65 U / ml despite severe hypoaggregability of SHRSP platelets in all the concentrations examined . - pubmed
These results suggest that abnormalities of SHRSP platelets at hypertensive ages are due to an impaired function of thromboxane A2 and/or calcium concerned in aggregation and secretion but not due to a defect in cyclo - oxygenase and thromboxane synthetase pathway . - pubmed
During exercise after propranolol a limited augmentation in cardiac performance and in myocardial and muscular blood flow was observed ; splanchnic blood flow which was most affected by propranolol at rest did not further decrease during exercise after propranolol . - pubmed
The influence of beta - adrenoreceptor blockade was even more evident under conditions of augmented sympathetic activity as reflected by a significant limitation of increase in cardiac performance , myocardial and muscular blood flow and by a sustained redistribution of splanchnic blood flow during exercise after propranolol . - pubmed
We developed an improved method for the dye - exclusion microcytotoxicity test by introducing low melting point agarose ( LMP - Aga ) into the assay medium . - pubmed
By contrast , both groups had similar serum levels of creatinine and beta 2 - microglobulin . - pubmed
Concerns about the redox reactivity of cell - free hemoglobin and its contribution to tissue - damaging oxygen free radicals has not been fully established . - pubmed
Superoxide radicals are produced during the spontaneous oxidation of hemoglobin . - pubmed
Spectral changes indicative of protein modification and ferrylhemoglobin formation during the enzymatic peroxidation of these hemoglobins differ qualitatively and occur at an increasing order , poly HbBv & gt ; HbBv & gt ; HbBv - FMDA . - pubmed
The proteins also differ in the rate of hemoglobin catalyzed NADPH oxidation and aniline hydroxylation , reactions mediated by reactive oxygen species . - pubmed
BACKGROUND : The likelihood of a positive response with dipyridamole stress echocardiography ( DSE ) is directly related to the extent and severity of angiographically assessed coronary artery disease . - pubmed
Antipyrine metabolism depends on at least three isoenzymes of cytochrome P450 forming the main metabolites 3 - OH - , 4 - OH- and norantipyrine . - pubmed
Our results demonstrate that the cytochrome P450 isoenzymes responsible for the different pathways of antipyrine metabolism are affected to different extents by cirrhosis . - pubmed
In a variety of systems , phosphatidylinositol 3 - kinase ( PI 3 - kinase ) is believed to be a downstream effector of tyrosine kinases , and activation of PI 3 - kinase results in increased levels of D3 - phosphorylated metabolites of phosphatidylinositol ( PtdIns ) . - pubmed
Increased levels of PtdIns 3,4,5 - P3 in LPS - treated cells resulted from an increase in the specific activity of PI 3 - kinase . - pubmed
Activation of PI 3 - kinase involved signaling through the monocyte cell surface molecule CD14 , since pretreatment of cells with Abs to CD14 abrogated LPS - induced increases in PtdIns 3,4,5 - P3 . - pubmed
Immunoprecipitates of p53 / 56lyn from LPS - treated cells showed a time - dependent and transient increase in PI 3 - kinase activity assayed in vitro , coordinate with activation of p53 / 56lyn , indicating that LPS induces the association and simultaneous activation of these two enzymes in vivo . - pubmed
These findings indicate that monocytes respond to LPS with the rapid activation of PI 3 - kinase , resulting in transient increases in levels of PtdIns 3,4,5 - P3 . - pubmed
Outcomes were levels of high - density lipoprotein cholesterol , insulin ( 2 hours after challenge ) , fibrinogen , systolic blood pressure . - pubmed
RESULTS : In white women , leisure physical activity was positively associated with levels of high - density lipoprotein cholesterol ( P = .001 ) and inversely associated with levels of insulin ( P = .04 ) and fibrinogen ( P = .02 ) . - pubmed
Compared with high - density lipoprotein cholesterol levels in the inactive and light leisure physical activity groups , moderate ( P & lt ; .001 ) and heavy ( P = .004 ) leisure activities were associated with higher high - density lipoprotein cholesterol levels . - pubmed
High - density lipoprotein cholesterol levels in the heavy leisure physical activity group were significantly higher than those in the moderate group ( P = .01 ) . - pubmed
Home physical activity was positively related to high - density lipoprotein cholesterol level ( P = .01 ) ; relative to lower levels of home physical activity , the heavy home physical activity group demonstrated significantly higher mean high - density lipoprotein cholesterol levels . - pubmed
Since there are so few H + present in the ECF in a free or a bound form , and in the absence of an increase in endogenous acid - production , the source of this H + is from proteins in the ICF . - pubmed
Accordingly , ALA - generated oxyradicals where shown to cause oxidative lesions in biomolecules and isolated cell organelles and to release iron from ferritin . - pubmed
[ Day to day changes in SAA ( serum amyloid A ) values of healthy subjects and the effect of dexamethasone administration ] . - pubmed
Cycloheximide and actinomycin D inhibited the ability of depudecin to induce a morphological change , suggesting that both mRNA synthesis and de novo protein synthesis are required for depudecin - mediated suppression of the transformed phenotypes in ras - transformed cells . - pubmed
Estrogen upregulation of BRCA1 expression with no effect on localization . - pubmed
The sex - steroid estrogen modulates cell proliferation of normal and neoplastic breast and ovarian epithelial cells , but the role of estrogen regulation on the expression of BRCA1 remains to be defined . - pubmed
In this study , estrogen - regulated BRCA1 expression was examined in breast and ovarian cancer cells . - pubmed
Cell fractionation studies localized the BRCA1 protein to the nucleus in both unstimulated and estrogen - stimulated cells . - pubmed
Taken together , these data support a role for the steroid estrogen and the involvement of the estrogen receptor pathway in the modulation of expression of BRCA1 . - pubmed
Removal of these portions by trypsin results in a fragment of 40 kDa ( HP40 ) , which lacks 42 NH2 - terminal and 51 COOH - terminal residues . - pubmed
Methionine aminopeptidase ( MetAP ) , in two isoenzymic forms , is responsible for hydrolysis of the initiator methionine residues from the majority of newly synthesized proteins . - pubmed
MetAP also has a potentially important role in the production of recombinant proteins , since failure to correctly remove initiator methionine residues can result in a product that is inactive or immunogenic . - pubmed
In contrast with previous methods , which usually use short tripeptides and require a post - reaction derivatization step , this assay uses peptides ranging in size from four to eight residues and utilizes UV detection of a tryptophan residue at the C - terminus . - pubmed
The mGluR antagonist ( R , S )- alpha - methyl-4 - carboxyphenylglycine ( MCPG ) was infused into the hippocampus 30 min before fear conditioning , and freezing was measured during both acquisition and retention tests . - pubmed
This shortening of the T1 - relaxation time is associated with pallidal deposition of manganese most likely reflecting the presence of an adaptive process designed to improve the efficacy of ammonia detoxification by astrocytes . - pubmed
Partial correction of anemia with iron , erythropoietin , or androgens can improve quality of life and potentially prevent cardiac disease . - pubmed
In addition to its action at cholinergic synapses acetylcholinesterase ( AChE ) has been proposed to modulate neuronal activity by mechanisms unrelated to the hydrolysis of acetylcholine . - pubmed
This increase is completely blocked by the specific AChE inhibitors propidium , physostigmine , DFP and BW 284C51 . - pubmed
AChE pretreatment had no effect on [ 3H]kainate binding . - pubmed
Furthermore , the AChE - induced potentiation of [ 3H]FW binding is Ca2 + - and temperature - dependent suggesting an enzymatic action for AChE in this system . - pubmed
Saturation binding experiments with [ 3H]FW to adult membranes reveal high and low affinity binding sites and demonstrate that the main action of AChE is to increase the Bmax of both sites . - pubmed
The objective of this study was to examine the association of carboxyhemoglobin ( COHb ) and plasma cotinine levels among pregnant women who smoke cigarettes and their fetuses . - pubmed
Maternal cotinine levels were correlated with maternal and fetal COHb levels ( r = 0.96 , p = 0.003 ; r = 0.99 , p = 0.0003 , respectively ) . - pubmed
Injections of both calcium channel blockers -- nifedipine ( 2.5 mg / kg ) and verapamil ( 5 mg / kg ) did not influence the ethanol - induced hypnotic activity themselves . - pubmed
It can be supposed that the modification of the activity of voltage - dependent calcium channels plays an important role in the anaesthetic action of ethanol . - pubmed
RESULTS : It was identified that a missense mutation in exon 6 of LPL gene ( 6G(979)-->A ) resulted in the substitution of Glu(242 ) by Lys in a heterozygous state . - pubmed
From the results of measurements of the nucleotide pools extracted from the antisense strain and its isogenic counterpart , we propose that one of the physiological roles of SGP is regulation and modulation of the GTP / GDP ratio under different growth conditions . - pubmed
Sensitivity of bacteriophage RB69 DNA polymerase to N2 -( p - n - butylphenyl )- 2 '- deoxyguanosine nucleotides . - pubmed
[ Amiloride - sensitive sodium channel in the nephron and its activation by vasopressin ] . - pubmed
PS1(P117L ) reduced BrdU incorporation the most ( 13 to 14 % ) followed by PS1(P267S ) ( 7.5 to 9 % ) , with the PS1(E280A ) mutant inhibiting BrdU incorporation the least ( 6 to 7 % ) , compared to wild - type PS1 . - pubmed
METHODS AND RESULTS : At a concentration of 1 mmol , only mercury showed significant inhibitory effects on the in vitro enzyme activities ; in all other cases , the enzymes remained active . - pubmed
Lipoxin - mediated inhibition of IL-12 production by DCs : a mechanism for regulation of microbial immunity . - pubmed
Phlorizin - specific binding and Western blot analysis indicated a decrease in the amount of SGLT1 in the ileum when compared to the duodenum and jejunum . - pubmed
We can conclude that the decrease in the apical D - glucose transport found in the ileum is primarily due to a reduction in the amount of SGLT1 present in the brush - border membrane rather than the differences in the lipid composition and fluidity . - pubmed
Suppression of monocyte chemoattractant protein 1 , but not IL-8 , by alprazolam : effect of alprazolam on c - Rel / p65 and c - Rel / p50 binding to the monocyte chemoattractant protein 1 promoter region . - pubmed
The goal of this study was to elucidate whether alprazolam modulates IL-1alpha - initiated responses . - pubmed
We found that alprazolam inhibited IL-1alpha - elicited MCP-1 production within a range of 0.1 - 3 micro M. In contrast , it did not inhibit IL-1alpha - induced IL-8 production . - pubmed
Although NF - kappaB is involved in regulating the IL-1alpha - induced expression of MCP-1 and IL-8 , the degradation of IkappaB - alpha stimulated by IL-1alpha was not inhibited by alprazolam . - pubmed
Alprazolam prevented NF - kappaB from binding to the MCP-1 promoter region ( the A2 and A1 oligonucleotide probes ) , but binding of NF - kappaB to IL-8 / NF - kappaB was not inhibited . - pubmed
Moreover , alprazolam inhibited c - Rel / p50 binding to the A2 oligonucleotide probe , but not p50 / p65 from binding to the IL-8 / NF - kappaB site . - pubmed
While AP-1 is involved in regulating the IL-1alpha - induced expression of IL-8 , but not MCP-1 , alprazolam potentiated the binding of c - Jun / c - Fos to the AP-1 oligonucleotide probe . - pubmed
These results show that the inhibition of IL-1alpha - mediated MCP-1 production by alprazolam is mainly due to inhibition of c - Rel / p65 and c - Rel / p50 binding to the MCP-1 promoter region , since alprazolam did not affect the IL-1alpha - mediated activation of NF - kappaB ( p50 / p65 ) or AP-1 ( c - Jun / c - Fos ) binding to the IL-8 promoter region . - pubmed
In conclusion , a new action of alprazolam was elucidated , as shown in the inhibition of c - Rel / p65- and c - Rel / p50 - regulated transcription . - pubmed
In addition cell death was prevented by antioxidants such as GSH , N - acetylcysteine or catalase . - pubmed
When the cells were exposed to MG132 the level of Bcl - XL diminished , while a new band , corresponding to the expression of the proapoptotic protein Bcl - XS was detected . - pubmed
MG132 also caused the release of cytochrome c from mitochondria and the activation of caspase-3 with the consequent degradation of poly - ADP ribose polymerase ( PARP ) . - pubmed
The observation that the broad spectrum caspase inhibitor z - VAD markedly reduced the apoptotic effect of the drug clearly demonstrated that caspases play an important role in MG132 - induced apoptosis . - pubmed
MG132 exerted a modest effect on the viability of Chang liver cells which primarily depended on the G2 / M arrest of cell cycle while only a small percentage of apoptotic cells was found . - pubmed
In response to ethylene , 9.5 cellulase was also induced in the vascular traces of the stem and the pulvinus without developing a separation layer . - pubmed
Low serum calcium was associated with elevated PTH- ( r = -0.75 , p = 0.001 ) , serum cross laps- ( r = -0.61 , p = 0.01 ) , osteocalcin levels ( r = -0.49 , p = 0.05 ) , was an independent predictor of femoral neck bone mass ( r = 0.57 , p = 0.02 ) and accounted for 36 % of this variance . - pubmed
Serum OPG was , however , significantly correlated to serum cross laps ( r = 0.71 , p = 0.003 ) , osteocalcin ( r = 0.63 , p = 0.01 ) , serum parathyroid hormone ( r = 0.61 , p = 0.01 ) and serum creatinine levels ( r = 0.53 , p = 0.04 ) and showed only a weak and non - significant correlation to femoral neck Z - scores ( r = -0.38 , p = 0.16 ) . - pubmed
Multiple regression analysis revealed that serum OPG was correlated independently of PTH , serum calcium and creatinine to serum cross - laps concentrations ( r = 0.63 , p = 0.04 ) . - pubmed
Liver fat content ( spectroscopy ) , serum insulin , C - peptide and triglyceride concentrations were significantly higher , and HDL cholesterol concentration lower in the HAART+LD+ than the HAART+LD- group . - pubmed
Dispersed anterior pituitary cells were incubated with or without fluorescein - conjugated CRH ( FL - CRH ) . - pubmed
[ Chloramphenicol - trypsin association in local treatment of chronic phlogosis of the sinal cavity and of the ear ] . - pubmed
Ligands of the TLR include exogenous microbial components such as LPS ( TLR4 ) , lipoproteins and peptidoglycans ( TLR1 , - 2 , - 6 ) , viral RNA ( TLR3 ) , bacterial and viral unmethylated cytosin - guanosin dinucleotide ( CpG)-DNA ( TLR9 ) , and endogenous molecules including heat - shock proteins and extracellular matrix molecules . - pubmed
Electron microscopy of the lens cytoskeleton from 129S2 / SvPas revealed similar morphological changes in the cytoskeleton as compared to the CP49 knockout , with beaded and intermediate filaments being apparently replaced by poorly defined filament - like material . - pubmed
Proteins secreted from the Ysa TTSS ( Ysps ) have only been detected in vitro when cells are cultured at 26 degrees C in a high - NaCl medium . - pubmed
Furthermore , in the absence of NaCl , the activity of ysaE - lacZ was reduced 25 - fold in the wild - type and DeltaysrS strains , indicating that YsrS is probably required for the salt - dependent expression of the ysa locus . - pubmed
Pre- and posttreatment with elaterium and cucurbitacin B reduced CCl4 - hepatotoxicity , as shown reduction in the anormally increased sGPT levels . - pubmed
Using tryptic phosphopeptide mapping , we have identified six additional CHK1 and CHK2 sites residing in the final 100 amino acids of p53 . - pubmed
Phosphorylation of at least three of these sites , Ser366 , Ser378 , and Thr387 , was induced by DNA damage , and the induction at Ser366 and Thr387 was abrogated by small interfering RNA targeting chk1 and chk2 . - pubmed
Exogenous creatine was taken up by keratinocytes and increased CK activity , mitochondrial function and protected against free oxygen radical stress . - pubmed
Upon butyrate treatment , the cyclin E - over - expressing cells exhibited a significantly increased albumin - secreting and ammonia - detoxifying capacity when compared to the control cells . - pubmed
OBJECTIVES : : The aims of this study were to improve the resolution of albumin variants ( bisalbumins , especially albumin Naskapi ) from albumin in commercial serum protein electrophoresis systems ; to compare the separation of bisalbumin from albumin on these improved methods against the recognized gold standard of capillary electrophoresis ; and to investigate the presence of albumin variants in urine . - pubmed
In response to changes in environmental factors such as oxygen tension , blood flow , circulating cytokines , and growth factors , the endothelium synthesizes and/or extracts many vasoactive mediators such as endothelin-1 ( ET-1 ) , norepinephrine , angiotensin 1 , thromboxane , prostacyclin ( PGI ( 2 ) ) , and the endothelial - derived relaxing factor nitric oxide ( NO ) . - pubmed
Among the PDF groups , cellular adhesion molecule expressions on ECs and CD11b expression on PMNs were lower with 600 and 1000 micromol / L than with 300 micromol / L GLN . - pubmed
IL-8 secretions from ECs and PMNs were higher with 300 and 600 micromol / L than with 1000 micromol / L GLN , and this was consistent with the expression of the IL-8 receptor on PMNs . - pubmed
A low GLN concentration comparable to catabolic conditions resulted in higher adhesion molecule expression and greater transendothelial migration of neutrophils . - pubmed
Body mass index ( BMI ) and lipid profile including total cholesterol , low density lipoprotein cholesterol , high density lipoprotein cholesterol and triglyceride correlated with blood pressure . - pubmed
RESULTS : The biological activity of PC - PLD was significantly decreased by TNF - alpha and ceramide ( P< ; 0.05 ) . - pubmed
On the other hand , the GMC apoptosis was induced by TNF - alpha and ceramide ( P & lt;0 . - pubmed
Methods We compared total nucleated cell ( TNC ) and CD34(+ ) cell counts before and after processing with three different CBU volume reduction methods used consecutively in our bank : a manual method based on hydroxyethyl starch sedimentation ( HES ) ( n=447 ) , a top - and - bottom ( TB ) semi - automated method ( n=181 ) using Optipress II , and the Sepax automated method ( n=213 ) . - pubmed
Gliding movements of the leading poles of cephalexin - treated filamentous cells were observed but not equivalent movements of the lagging poles . - pubmed
Indole-2 - amide based biochemical antagonist of Dishevelled PDZ domain interaction down - regulates Dishevelled - driven Tcf transcriptional activity . - pubmed
We designed and synthesized a series of indole-2 - amide - based compounds that antagonize interaction between the Dishevelled ( Dvl ) PDZ domain and a peptide derived from the natural PDZ ligand Frizzled-7 ( Fz7 ) . - pubmed
46 : 1303 - 1311 ) characterized the properties of secondary structure and in vitro functions of different cysteine mutants of apolipoprotein A - I. - pubmed
Chondrocytes stimulated with tumor necrosis factor - alpha ( TNF - alpha ) plus Ro 31 - 8220 ( a specific inhibitor of mitogen - activated kinase phosphatase-1 - MKP-1 ) were employed as an in vitro model of apoptosis . - pubmed
Protein phosphatase 2A ( PP2A ) activity was estimated by measuring the absorbance of a molybdate : malachite green : phosphate reaction complex . - pubmed
The integral membrane subunit PsrC was characterized using the natural substrate menaquinone-7 and inhibitors , providing a comprehensive representation of a quinone binding site and revealing the presence of a water - filled cavity connecting the quinone binding site on the periplasmic side to the cytoplasm . - pubmed
SIGNIFICANCE : The degree of nanoleakage had no influence on microtensile bond strength for the Syntac classic / Tetric Ceram Cavifil or for the Prime & amp ; Bond NT / Spectrum TPH group . - pubmed
BACKGROUND : The aim of this study was first to compare the widely used flow mediated dilation ( FMD ) method with the iontophoretically induced acetylcholine vasodilation ( IAV ) procedure . - pubmed
Part II : Isolation of the linear 9 - formylcarboxylic acid and its sequential macrocyclization with an amino alcohol or an azidoamine . - pubmed
Although optimization of 15 - membered azalactams including demycarosyl analogues did not provide remarkably promising molecules , SAR studies of 16 - membered azalides disclosed that substitution at the 15 position was very important for identification of a clinical candidate . - pubmed
The 5 ' triphosphate - ended virus leader transcript is recognised by RIG - I , a cellular helicase , and induces the interferon response . - pubmed
One - dimensional ( 1 ) H magnetic resonance spectroscopy ( MRS ) experiments were performed on the supernatant of aqueous dispersions of the stool samples . - pubmed
Using SR1 - specific 16S rRNA gene primers , we identified multiple novel SR1 lineages in four different anaerobic environments : sediments from Zodletone Spring , a sulfide- and sulfur - rich spring in southwestern Oklahoma ; inner layers of microbial mats obtained from Sperm Pool , a high - temperature , low - pH pool ( 55 degrees C , pH 2.5 ) in Yellowstone National Park ; fresh bovine ruminal contents ; and anaerobic freshwater pond sediments ( Duck Pond ) in Norman , Oklahoma . - pubmed
Corneal verticillata after dual anti - epidermal growth factor receptor and anti - vascular endothelial growth factor receptor 2 therapy ( vandetanib ) for anaplastic astrocytoma . - pubmed
Protein denaturation is preceded by an initial loss of water from the first solvation shell and consequent in - flow of urea toward the protein . - pubmed
Urea reaches the first solvation shell of the protein mainly due to electrostatic interaction with a considerable contribution coming from the dispersion interaction . - pubmed
We have also seen that water follows urea and reaches the protein interior at later stages of denaturation , while urea preferentially and efficiently solvates different parts of the protein . - pubmed
Why urea is preferred over water for binding to the protein backbone and how urea orients itself toward the protein backbone have been identified comprehensively . - pubmed
Tumors with 13q34 amplification were associated with high grade , estrogen receptor negativity , and expression of EGFR , CCNE , CK5 , and P - Cadherin , among other basal cell markers . - pubmed
A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population . - pubmed
AIM : To compare the efficacy of different solutions ( 1 % sodium hypochlorite , 10 % citric acid and 20 % EDTA ) in the removal of calcium hydroxide from root canals . - pubmed
Following the selection of reference genes , the novel target genes E2F7 and IL-11RA were identified as potential toxicity biomarkers for acetaminophen treatment . - pubmed
grisea infection and following exogenous jasmonate ( JA ) or salicylic acid ( SA ) exposure in the old leaves , but not in the new leaves of the wild - type . - pubmed
These are : PenB_TUA1 coding for an alpha - tubulin family protein , PenB_Raba1/11 coding for a Rab family protein , PenB_HMG - box coding for an HMG - box family protein and PenB_MT coding for an unknown transcript that contains an ORF of 295 amino acid residues . - pubmed
Small - angle X - ray diffraction ( SXRD ) , infrared spectroscopy ( IR ) , nitrogen adsorption / desorption , transmission electron microscopy ( TEM ) , thermogravimetric analysis ( TGA ) , and inductively coupled plasma atomic emission ( ICP - AES ) measurements allow us to propose that the organotin species are located within the pore channels of the mesoporous host . - pubmed
Results of redundancy analysis ( RDA ) showed that silicate ( SiO ( 4 ) ) and soluble reactive phosphorus ( SRP ) were the most important environmental factors influencing the phytoplankton distribution during spring and summer , respectively . - pubmed
Comparison of peritonsillar infiltration effects of ketamine and tramadol on post tonsillectomy pain : a double - blinded randomized placebo - controlled clinical trial . - pubmed
AIM : To assess the effect of peritonsillar infiltration of ketamine and tramadol on post tonsillectomy pain and compare the side effects . - pubmed
CONCLUSION : Preoperative peritonsillar infiltration of tramadol can decrease post - tonsillectomy pain , analgesic consumption , and the time to recovery without significant side effects . - pubmed
Levels of PGC-1alpha were correlated negatively ( P<0.05 ) with total cholesterol levels , aortic superoxide anion production and tumour necrosis factor - alpha expression , and positively ( P<0.05 ) with maximal relaxation in response to acetylcholine . - pubmed
OBJECTIVES : Tacrine was the first acetylcholinesterase inhibitor approved for the treatment of Alzheimer disease . - pubmed
Parameters such as ferric reducing antioxidant power ( FRAP ) , thiobarbituric acid reactive substances ( TBARS ) , carbonylated proteins , caspase 3 activity , superoxide dismutase activity and glutathione reductase activity were assessed in the frontal , temporal and occipital lobe , cerebellum , liver , spleen , heart , and kidney . - pubmed
Moreover , levels of glucose , total and HDL cholesterol forms , triglycerides , blood urea nitrogen , creatinine , total bilirubin , total protein , albumin and activities of aspartate aminotransferase , alanine aminotransferase , alkaline phosphatase and lactate dehydrogenase were assessed in plasma samples . - pubmed
Subsequently , we demonstrate that LPA is a potent mast cell activator which , unlike other triggers , favors release of tryptase . - pubmed
In this study , we report that Adenine Phosphoribosyl Transferase 1 ( APT1 ) is the causal gene of the high - dose cytokinin - resistant mutants . - pubmed
After accounting for age and sex , DHEAS was significantly inversely associated with both anxiety sensitivity ( F(3,92 ) = 6.97 , partial eta2 = 0.07 , P = 0.01 ) and negative affect ( F(3,87 ) = 10.52 , partial eta2 = 0.11 , P = 0.002 ) across groups . - pubmed
The persistent significance of the androgen receptor ( AR ) axis in CRPC was recently validated by the clinical efficacy of androgen synthesis inhibitors ( abiraterone ) and novel , second - generation AR antagonists ( enzalutamide ) . - pubmed
The appreciation that conventional therapeutic approaches achieve a suboptimal ablation of intratumoral androgens and AR axis signaling output opens transformative therapeutic opportunities . - pubmed
The levels of AST , CK , ALT , SOD , GSH - pX , MDA , miR-146a , and ECG were measured to explore the effects of SMI in cardiologic function and immune activity . - pubmed
RESULTS : Results show that the levels of AST , CK , and MDA decreased upon the administration of SMI . - pubmed
The APS reveals unique optical and electronic properties which can efficiently modify the work function ( PhiW ) of indium tin oxide ( ITO ) electrode to enhance the electron blocking capability . - pubmed
The inverted device based on APS reached a power conversion efficiency ( PCE ) of 3.56 % and exhibited much better stability under ambient conditions relative to that of the corresponding PEDOT : PSS based device . - pubmed
Gene network modeling and characterization of T - DNA mutants indicated that acyl - activating enzyme encoded by At4g19010 contributes to the biosynthesis of ubiquinone specifically from phenylalanine . - pubmed
CoA ligase assays verified that At4g19010 prefers para - coumarate , ferulate , and caffeate as substrates . - pubmed
Feeding experiments demonstrated that the at4g19010 knockout can not use para - coumarate for ubiquinone biosynthesis and that the supply of 4 - hydroxybenzoate , the side - chain shortened version of para - coumarate , can bypass this blockage . - pubmed
Furthermore , a trans - cinnamate 4 - hydroxylase mutant , which is impaired in the conversion of trans - cinnamate into para - coumarate , displayed similar defects in ubiquinone biosynthesis to that of the at4g19010 knockout . - pubmed
Green fluorescent protein fusion experiments demonstrated that At4g19010 occurs in peroxisomes , resulting in an elaborate biosynthetic architecture where phenylpropanoid intermediates have to be transported from the cytosol to peroxisomes and then to mitochondria where ubiquinone is assembled . - pubmed
Collectively , these results demonstrate that At4g19010 activates the propyl side chain of para - coumarate for its subsequent beta - oxidative shortening . - pubmed
Expression of serine peptidase inhibitor , Kazal type 3 ( Spink3 ) was detected in EPL cell - derived endoderm , and in a band of endoderm immediately distal to the embryonic - extra - embryonic boundary in pregastrula and gastrulating embryos . - pubmed
The gammadeltaT cells that were positive for CCR4 were killed by mogamulizumab via ADCC . - pubmed
This suggests that the mechanism by which melatonin alleviates the damage to the spinal cord by edema might be related to the expression of aquaporin-4 and glial fibrillary acidic protein . - pubmed
Poly - dipeptides encoded by the C9ORF72 repeats block global protein translation . - pubmed
Among these , poly - proline - arginine ( poly - PR ) and poly - glycine - arginine ( poly - GR ) peptides are known to be neurotoxic . - pubmed
The results displayed that the AMPKalpha1 overexpression suppressed lipid accumulation in the cytoplasm , decreased triglyceride levels , maintained the survival of steatotic hepatocyte model with decreased cell apoptosis and increased survival rate . - pubmed
Finally , Anisomycin , a frequently - used activator of p38MAPK , reversed the inhibitory effect of pc - AMPKalpha1 on the expression of p - p38MAPK , suggesting that AMPKalpha1 overexpression alleviates inflammatory response through the inactivation of p38MAPK . - pubmed
Serum and Plasma Levels of Vascular Endothelial Growth Factors in Relation to Quality of Glucose Control , Biomarkers of Inflammation , and Diabetic Nephropathy . - pubmed
Levels of vascular endothelial growth factors ( VEGF ) are regulated in a complex network of adipokines , glucose control , and low grade inflammation together with activated platelets , leucocytes , and endothelial dysfunction . - pubmed
T2DM was associated with significantly higher levels of hsCRP , ALAT , and albumin / creatinine ratio . - pubmed
Estrogen , relaxin , and progesterone can alter the balance between tissue degradation and repair by modulating MMPs , leading to gender disparities in many MMP - related disease states . - pubmed
In this article , we review the literature that examines the effects of estrogen , relaxin , and progesterone on MMP production and activity , connecting the nanoscale , microscale , and macroscale details to relevant disease states . - pubmed
Once successful transfection was achieved , the effects on the mRNA level of human telomerase reverse transcriptase ( hTERT ) , telomerase activity , cell proliferation and apoptosis were examined by semi - quantitative RT - PCR , stretch PCR , MTT assay and flow cytometry , respectively . - pubmed
Phytochemical profiling of the most active fraction by nontargeted RP - HPLC - MS and MS / MS technique showed that rosmarinic acid and methylrosmarinate constituted nearly 51 % of the total metabolites apart from twelve other major chemotypes . - pubmed
MATE2 Expression Is Associated with Cancer Cell Response to Metformin . - pubmed
Intracellular uptake and retention of metformin is affected by the expression of organic cation transporters ( OCT ) 1 - 3 and by multidrug and toxic compound extrusion ( MATE ) 1 - 2 . - pubmed
PURPOSE : Here we sought to determine if there is an association between the effects of metformin on inhibiting oxygen consumption , proliferation and expression of OCTs and MATEs in a panel of 19 cancer cell lines . - pubmed
There were statistically significant associations between resistance to inhibition of proliferation and MATE2 expression , as well as between sensitivity to inhibition of oxygen consumption and OCT3 expression . - pubmed
High MATE2 expression may result in resistance to the anti - proliferative effect of metformin and should be considered as a negative predictive biomarker in clinical trials . - pubmed
EglN2 contributes to ERalpha - positive breast tumorigenesis by acting as an estrogen - inducible gene . - pubmed
Furthermore , we identified some potential serine or threonine residues on the C - terminal of EglN2 that may mediate its binding and potential regulation by FBW7 . - pubmed
In regards to T2DM - related metabolic complications , the AG and GG genotypes of rs731236 were significantly associated with higher total cholesterol ( p=0.011 ) and LDL - cholesterol ( p=0.009 ) levels in the patients with T2DM . - pubmed
In contrast , the CT genotype of rs1544410 was significantly associated with lower BMI ( p=0.031 ) and the TT genotype was associated with lower LDL - cholesterol level ( p=0.007 ) . - pubmed
The rs731236 variant was shown to be associated with high cholesterol and LDL - cholesterol levels in T2DM patients , while rs1544410 was associated with lower BMI and lower LDL cholesterol levels . - pubmed
These preliminary results of biexponential T2 analysis could potentially be used to increase the specificity for detection of early osteoarthritis by measuring different water compartments and their fractions . - pubmed
CONCLUSIONS : In this preclinical model , the new biodegradable polymer - based DES studied showed less restenosis than BMS and no significant differences in safety or efficacy vs commercially available DES . - pubmed
Sensitive and Label - Free Fluorescent Detection of Transcription Factors Based on DNA - Ag Nanoclusters Molecular Beacons and Exonuclease III - Assisted Signal Amplification . - pubmed
Herein , we first applied DNA - Ag nanoclusters molecular beacons ( AgMBs ) in TFs analysis and designed an assay based on the switchable fluorescence of AgMBs . - pubmed
Then , the reporter triggers downstream Exo III - assisted signal amplification by continuously consuming the guanine - rich enhancer sequences in AgMBs , resulting in significant fluorescent decrease eventually . - pubmed
DNA was extracted from peripheral white blood cells and methylation levels of nine CpG sites in NET gene promoter region were investigated by pyrosequencing . - pubmed
RESULTS : For each CpG site and the average value of nine CpG sites , there were no significant differences in DNA methylation of the NET gene promoter between healthy controls and patients with depression or hypertension . - pubmed
CONCLUSION : Our study indicates that DNA methylation levels of nine CpG sites in NET gene promoter region are not associated with depression and hypertension . - pubmed
Deficient Insulin - mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy . - pubmed
Therefore , we tested a link between insulin deficiency and dysregulated activity of the equilibrative nucleoside transporters ( ENTs ) responsible for controlling extracellular levels of adenosine . - pubmed
In ex vivo glomeruli , high D - glucose decreased nucleoside uptake mediated by ENT1 and ENT2 transporters , resulting in augmented extracellular levels of adenosine . - pubmed
Particularly , insulin through insulin receptor / PI3 K pathway markedly upregulated ENT2 uptake activity to restores the extracellular basal level of adenosine . - pubmed
In conclusion , we evidenced ENT2 as a target of insulin signaling and sensitive to dysregulation in diabetes , leading to chronically increased extracellular adenosine levels and thereby setting conditions conducive to kidney injury . - pubmed
Identification of Novel Conjugative Plasmids with Multiple Copies of fosB that Confer High - Level Fosfomycin Resistance to Vancomycin - Resistant Enterococci . - pubmed
The total copy number of the fosB gene as revealed by qRT - PCR did not correlate with fosfomycin MICs or growth rates at sub - MICs of fosfomycin in different transconjugants . - pubmed
From susceptibility tests , the fosB gene , regardless of the copy number , conferred high fosfomycin MICs that ranged from 16384 to 65536 mug / ml . - pubmed
This first complete nucleotide sequence of a plasmid carrying two copies of fosB in VRE suggests that the fosB gene can transfer to multiple loci of plasmids by the ISL3 family transposase TnpA , possibly in the form of circular intermediates , leading to the dissemination of high fosfomycin resistance in VRE . - pubmed
A combined computational / experimental study on HSA binding of two water - soluble Schiff base ligands derived from pyridine derivative and ethylendiamine . - pubmed
The efficacy of erlotinib treatment for advanced non - small cell lung cancer ( NSCLC ) is due to its action as an epidermal growth factor receptor tyrosine kinase inhibitor ( EGFR - TKI ) . - pubmed
The single - nucleotide polymorphisms ( SNPs ) rs1042640 in UGT1A10 , rs1060463 , and rs1064796 in CYP4F11 , and rs2074900 in CYP4F2 were significantly associated with therapeutic responses to erlotinib . - pubmed
Moreover , analysis of a validation cohort confirmed the significant association between rs10045685 in UGT3A1 and erlotinib adverse drug response(unadjusted p = 0.015 ) . - pubmed
The aim of the present study was to formulate a nanosuspension ( FA - NS ) of fusidic acid ( FA ) to enhance its aqueous solubility and antibacterial activity . - pubmed
The molecular dynamics simulation of FA and Poloxamer-188 showed an interaction and binding energy of -74.42 kJ / mol and -49.764 + - 1.298 kJ / mol , respectively , with van der Waals interactions playing a major role in the spontaneous binding . - pubmed
The MTT assays showed a cell viability of 75 - 100 % confirming the nontoxic nature of FA - NS . - pubmed
Bioavailability was determined by co - administering feed with either pure PZQ , HCO - SLN or HCO - SLN coated with chitosan via intubation and quantifying the pharmacokinetics response . - pubmed
Furthermore , palatability of the feeds medicated with PZQ was not improved when the PZQ was incorporated into HCO - SLN , possibly due to the low loading rate of PZQ within the HCO - SLN and the subsequent thick coating of nanoparticles that was required on the surface of the feed pellets . - pubmed
Lenalidomide is approved for maintenance and guidelines recommend its use post ASCT . - pubmed
In addition to their classical mode of action in the brain , circulating factors may modulate the release of reactive oxygen / nitrogen species ( ROS / RNS ) from endothelial cells that compose the BBB without entering the brain . - pubmed
Control and early life stressed heterozygous serotonin transporter knockout ( SERT ) dams were treated with the SSRI fluoxetine or vehicle throughout gestation and lactation . - pubmed
Fluoxetine treatment of the dams resulted in a reduced total USV duration in pups at postnatal day 6 , especially in SERT+/+ males . - pubmed
Interestingly , early life stress in dams increased adult social exploration in vehicle exposed SERT+/+ female offspring and total social behavior in fluoxetine exposed adult SERT+/- male offspring . - pubmed
The enzymes L - asparaginase , L - glutaminase , and L - arginase were chosen according to amino acids starvation in cancer cells and screened in halophilic and halotolerant bacteria , given probably less immunological reactions of halophilic or halotolerant enzymes in patients . - pubmed
Identification of personalized antihypertensive treatments is a key for better BP management Objective : This review aimed to elucidate aspects of rational drug design and other methods to develop better hypertension management Results : Among hypertension - related signaling mechanisms , the renin - angiotensin - aldosterone system is the leading genetic target for hypertension treatment . - pubmed
The excessive accumulation of triglyceride ( TG ) and the deposition of fat were the two most critical causes . - pubmed
Here , we developed Fe3O4@OA@Poloxamer nanoparticles ( NPs ) with amphiphilic structures , which exhibited an excellent role in eliminating excess TG . - pubmed
Hydrophobic TG was adsorbed efficiently by Fe3O4@OA@Poloxamer NPs through the " liposuction effect " and the formation of NPs@TG complex was then conducted . - pubmed
Meanwhile , the " nano - enzyme effect " of Fe3O4 NPs recovered the lipid - regulated proteins including PPARalpha , further triggering biodegradation pathways of TG , although the lipid - regulated proteins were obviously inhibited in the high - fat hepatocytes models . - pubmed
luteum , possessing epigallocatechin , were active inhibitors of cholinesterase enzymes , alpha - glucosidase , and tyrosinase . - pubmed
The data presented here include sea surface temperature ( SST ) , sea surface salinity ( SSS ) , Secchi disk depth ( SDD ) , Chlorophyll - a ( Chl - a ) , suspended particulate matter ( SPM ) , mineral suspended solid ( MSS ) and chromophoric dissolved organic matter ( CDOM ) . - pubmed
To overcome barriers associated with gene delivery , a series of peptides consisting of multifunctional fragments , including a cationic amphiphilic alpha - helical antimicrobial peptide ( AMP ) , a cell penetrating peptide ( CPP ) , TAT , a stearyl moiety , and cysteine residues , were designed and synthesized for evaluation as non - viral gene vectors . - pubmed
Stearyl moieties provide an intramolecular hydrophobic environment to promote AMPs to form an alpha - helical conformation in PBS , and this is beneficial for DNA binding , cellular uptake , and endosomal escape . - pubmed
64Cu - labeled new pan - somatostatin receptors ( pan - SSTRs ) probe PA1 was synthesized , characterized , and evaluated by in vitro and in vivo experiments . - pubmed
The radiosynthesis , quality control , and preliminary biological evaluation of [ 64Cu ] NOTA - PA1 have broaden the application of radiolabeled octreotide for SSTRs imaging , which could act as a potential multisubtypes targeted radiotracer for imaging SSTRs - positive tumors . - pubmed
The biosynthetic pathways for flavonoids and terpenoids are well represented in roots , including the complete set of proteins for the MEV and MEP / DOXP pathways , and proteins involved in the branches which lead to the synthesis tricyclic diterpenoids and gibberellins . - pubmed
Also , casbene synthase which catalyzes the first commited step in the biosynthesis of tigliane - type diterpenes was identified in roots of both genotypes , but not in leaves and endosperm . - pubmed
Cerebral Ischemia - Reperfusion Injury : Lysophosphatidic Acid Mediates Inflammation by Decreasing the Expression of Liver X Receptor . - pubmed
The levels of LPA , an extracellular signaling molecule , increased after ischemia and caused neurological injury effect , decreased the expression level of LXR , and increased the expression level of inflammatory factors ( IL-1beta , IL-6 , and TNF - alpha ) via the NFkappaB signaling pathway . - pubmed
We present a direct or indirect connection between LPA and LXR in the pathophysiological process . - pubmed
In conclusion , we speculate that the inhibition of LPA generation and administration of LXR agonist may be explored as potential cerebral infarction treatment strategies . - pubmed
Analysis using the Kyoto Encyclopedia of Genes and Genomes database revealed differential genes associated with ribosome function , pyruvate metabolism , biosynthesis of amino acids , fatty acid biosynthesis , fatty acid metabolism , ATP - binding cassette ( ABC ) transporter , glycolysis , and glycerophospholipid metabolism . - pubmed
RESULTS : The LN , active LN , and stable LN groups had significantly higher levels of anti - dsDNA antibody , C - reactive protein , serum creatinine ( Scr ) , and blood urea nitrogen ( BUN ) than the control group ( P<0.05 ) . - pubmed
A potential to extend this versatility further was expected and examined in the present study , based on the recently discovered hysteretic behavior of a widely used chiral selector ( CS ) , amylose tris ( 3,5 - dimethylphenylcarbamate ) . - pubmed
Resveratrol prevents TNF - alpha - induced VCAM-1 and ICAM-1 upregulation in endothelial progenitor cells via reduction of NF - kappaB activation . - pubmed
OBJECTIVE : To assess the effects of resveratrol ( RSV ) on expression of adhesion molecules in endothelial progenitor cells ( EPCs ) following tumor necrosis factor - alpha ( TNF - alpha ) stimulation . - pubmed
A total of 10 - 20 % of plasma membrane proteins are anchored by glycosylphosphatidylinositol ( GPI ) . - pubmed
The median values of HBsAg reduction from baseline were -0.44 ( n=48 ) , -0.41 ( n=40 ) , and -0.68 ( n=11 ) log IU / mL at the end of treatment and at 48 and 144 weeks post - treatment , respectively . - pubmed
The rates of HBsAg response were 24.0 % and 22.5 % at the end of treatment and at 48 weeks post - treatment , respectively . - pubmed
A multivariate analysis identified HBsAg < 3.00 log IU / mL as an independent baseline factor contributing to the HBsAg response at the end of treatment and 48 weeks post - treatment ( p=1.07x10 - 2 and 4.42x10 - 2 , respectively ) . - pubmed
There were significant differences in the reduction of the HBsAg levels at 12 weeks of treatment and in the incidence of serum ALT increase during treatment between patients with and without an HBsAg response . - pubmed
Apoferritins prepared chemically by removal of iron from the holoferritins had migration properties indistinguishable from the parent holoferritins . - pubmed
There is an increase in the serum thyroxine ( T4)and triiodothyronine ( T3 ) concentration largely due to an increase in thyroid hormone - binding proteins . - pubmed
Six male chronic marihuana ( MH ) users exercised on a bicycle ergometer for 15 min at approximately 50 % VO2max under 3 conditions : ( 1 ) not smoking ( control ) , ( 2 ) after smoking MH containing 7.5 mg ( - ) delta-9 - tetrahydrocannabinol , and ( 3 ) after smoking placebo marihuana ( PL ) . - pubmed
The combined effect of radiotherapy and neuraminidase - treated tumor cells on 3 - methylcholanthrene - induced fibrosarcoma . - pubmed
Somatostatin delayed the onset of the motilin response and reduced motor activity in the stomach and the jejunum . - pubmed
During somatstatin infusion the motilin - induced contractile pattern did not migrate to the jejunum . - pubmed
The effects of sucrose uptake on the degradation of total cellular protein and of the cytoplasmic enzyme , alkaline ribonuclease , were determined for comparative purposes . - pubmed
Sucrose uptake produced increases of 270 % and 90 % respectively in the rates of synthesis of arylsulphatase B and galactosyltransferase , whereas the rate of synthesis of alkaline ribonuclease was not affected . - pubmed
In the absence of sucrose , the apparent half - lives of arylsulphatase B and galactosyltransferase were about 30 days and 40h respectively . - pubmed
After uptake of sucrose , the half - life of arylsulphatase B decreased to about 10 days and that of galactosyltransferase to 10h . - pubmed
Neither the half - life of alkaline ribonuclease ( 4 days ) nor the rate of degradation of total cellular protein was affected by the uptake of sucrose . - pubmed
Our investigations demonstrated a dose dependent blood glucose decrease with each sulfonylurea corresponding with the insulin levels after intravenous application . - pubmed
Gliquidone revealed a " tolbutamide - typed " dynamic both on insulin secretion and on blood glucose decrease whereas gliflumide showed a " glibenclamide - typed " reaction . - pubmed
The delayed and more prolonged insulin decrease , well known for glibenclamide , was even more distinct following gliflumide . - pubmed
Differences of insulin secretion after sulfonulureas can be observed only under intravenous conditions . - pubmed
St 587 ( 0.5 - 30 micrograms ) decreased TF latency and PP threshold below base line . - pubmed
doses of St 587 & gt ; 3 micrograms , there was an apparent but incomplete return of TF latency and PP threshold toward base line . - pubmed
guanfacine ( alpha 2 agonist ) in the TF and PP tests . - pubmed
Mechanism - based inactivation of dihydropyrimidine dehydrogenase by 5 - ethynyluracil . - pubmed
Uracil analogues with appropriate substituents at the 5 - position inactivated dihydropyrimidine dehydrogenase ( DHPDHase ) . - pubmed
For example , 5 - ethynyluracil inactivated DHPDHase with an efficiency ( kinact / Ki ) that was 500 - fold greater than that for 5 - propynyluracil . - pubmed
5 - Ethynyluracil inactivated DHPDHase by initially forming a reversible complex with a Ki of 1.6 + /- 0.2 microM. - pubmed
Thymine competitively decreased the apparent rate constant for inactivation of DHPDHase by 5 - ethynyluracil . - pubmed
The absorbance spectrum of 5 - ethylnyluracil - inactivated DHPDHase was different from that of reduced enzyme . - pubmed
5 - Ethynyluracil inactivated DHPDHase with a stoichiometry of 0.9 mol of inactivator per mol of active site . - pubmed
Enzyme inactivated with [ 2 - 14C ] 5 - ethynyluracil retained all of the radiolabel after denaturation in 8 M urea , but lost radiolabel under acidic conditions . - pubmed
A radiolabeled peptide was isolated from a tryptic digest of the enzyme inactivated with [ 2 - 14C ] 5 - ethynyluracil . - pubmed
The amino acid residue designated by X was identified as a cysteine from previous work with DHPDHase inactivated with 5 - iodouracil . - pubmed
In contrast to 5 - ethynyluracil , 5 - cyanouracil was a reversible inactivator of the enzyme . - pubmed
5 - Cyanouracil - inactivated enzyme slowly regained activity ( t1/2 = 1.8 min ) after dilution into the standard assay . - pubmed
The study of hormonal balance in the presence of pregnancy and labor in females with a history of infertility treated with parlodel failed to reveal any negative effect of elevated prolactin levels on the fetoplacental system . - pubmed
Sucrose gradient centrifugation of the PIPLC - released AChE showed a single 8.3 S molecular form , similar to that observed for AChE solubilized by Triton X-100 . - pubmed
PIPLC removed large amounts of AChE from the surface of intact schistosomula in culture , with no impairment of the viability of the parasite . - pubmed
Inhibition experiments with carbon monoxide and n - octylamine indicated that this sulphoxidation is catalyzed by cytochrome ( s ) P-450 , whereas flavin - containing monooxygenase and/or non - enzymatic reactions ( via peroxides ) seems not to be involved : no inhibition was observed by methimazole , N , N - dimethylaniline , preheating or glutathione and EDTA . - pubmed
Aristolochic acid is a non - competitive inhibitor of PLA2 . - pubmed
The binding of aristolochic acid to PLA2 induces an extrinsic CD band at 320 nm . - pubmed
Aristolochic acid forms a 1:1 complex with PLA2 , with an association constant K , of 5.4 X 10(3 ) M-1 and a Gibb 's free energy change ( delta G0 ) for the reaction of -5.1 kcal / mole . - pubmed
Binding of aristolochic acid causes a change in the secondary structure of the protein which is characterized by an increase in the apparent content of alpha - helix , without any detectable change in the tertiary structure of PLA2 . - pubmed
Whereas steroids exerted limited , possibly dose - dependent effects on this sequence , cyclophosphamide seemed to cause normalization of OKT4+/OKT8 + ratio , precipitous drop of anti - ds - DNA - Ab titers , and dramatic clinical improvement . - pubmed
p - Phenylenediamine , o - dianisidine ( o - DA ) and 3,5,3 ' , 5 '- tetramethylbenzidine were found to be optimal substrates of horse - radish peroxidase . - pubmed
The kinetics of oxidation of these substrates by horseradish peroxidase modified with three molecules of Strophanthin K was studied as well . - pubmed
Within the temperature range from 37 to 53 degrees C the inactivation rate constants were determined for peroxidase and its conjugate with Strophanthin K. The effect of sugars and polyols on thermal stability of the conjugate peroxidase - Strophanthin K was investigated . - pubmed
Measurements of glucose utilization during constant infusions of insulin , showed significant changes with changing energy state , falling with overeating and rising with undereating . - pubmed
Insulin sensitivity was not correlated with plasma norepinephrine metabolism . - pubmed
We have compared surface charge and the surface charge density on the polyanions heparin and potassium polyvinyl sulfate ( KPVS ) , as well as on hydrolyzed heparin and KPVS , with their accelerating effect on the inhibitory action of antithrombin III on thrombin . - pubmed
When the post - phenobarbitone half - lives were adjusted to a standard height they were approximately normally distributed . - pubmed
There was a significant regression of mean offspring value on mid - parent value , indicating that about 65 % of the observed phenotypic variance of post - phenobarbitone plasma phenylbutazone half - lives is due to the additive effects of genes . - pubmed
We measured CBF by a modification of the venous occlusion plethysmography technique and found that CBF increased 7.8 % per Torr alveolar carbon dioxide pressure change and that it decreased 15 % with 100 % O2 . - pubmed
These findings suggest that 1 ) CO2 is an important regulator of CBF in the perterm infant , 2 ) CBF - CO2 sensitivity in these infants may be greater than in adult subjects , 3 ) 100 % O2 reduced CBF significantly , and 4 ) a decrease in CBF during administration of 100 % O2 may be at least partially responsible for the increase in ventilation with hyperoxia . - pubmed
In the complicated cases of myocardial infarction it was established that the extraction of glucose , insulin decrease and in a number of cased production of lactate by the myocardium supervenes . - pubmed
The mean maternal serum glucose was 124.3 mg / dl ( range : 72.7 to 281.5 mg / dl ) , the mean hemoglobin A1c was 6.03 % ( range : 3.3 to 11.0 % ) and the mean fructosamine level was 255.97 mumol / L ( range : 205 to 397 mumol / L ) . - pubmed
Additionally , no significant correlation was noted between RI values and serum glucose levels ( r = 0.03 ) , hemoglobin A1c levels ( r = 0.13 ) or fructosamine levels ( r = 0.04 ) during the period of 26 to 34 gestational weeks . - pubmed
Since the intracellular activity of glutamine synthetase is significantly decreased after the administration of APAP to hepatocytes in culture , it is likely that the arylation of this enzyme may be involved in the ensuing hepatotoxicity . - pubmed
The enzyme was proven to be a metalloprotease like protease III and IDE , as was shown by the inhibitory effects exerted by EDTA and o - phenanthroline . - pubmed
Similar to protease III and IDE , ICP prefers the cleavage of small polypeptides ( insulin , insulin B - chain , glucagon ) to the cleavage of proteins ( casein , human serum albumin , globin ) and was inactive against synthetic amino acid derivates ( esters , p - nitranilides , and furoylacroleyl substrates ) of subtilisin , thermolysin , trypsin , and chymotrypsin . - pubmed
Cleavage sites in the oxidized insulin B - chain generated by ICP are Asn3 - Gln4 , His10 - Leu11 , Ala14 - Leu15 , Leu17 - Val18 , Gly23 - Phe24 , Phe24 - Phe25 , and Phe25 - Tyr26 . - pubmed
Repression of angiotensin II and potentiation of bradykinin contribute to the synergistic effects of dual metalloprotease inhibition in heart failure . - pubmed
Blockade of the formation of angiotensin II ( Ang II ) acting together with potentiation of the natriuretic peptides , bradykinin and other vasoactive peptides may mediate the interaction of dual enzyme inhibition . - pubmed
The Ang II AT1 receptor antagonist , SR 47436 ( BMS-186295 ) , was administered at 30 mumol / kg , i.v . - pubmed
infusion at 1 mumol / kg / min in combination with NEP - I ( SQ-28603 at 30 mumol / kg i.v . ) - pubmed
Bradykinin B2 receptor antagonism by Hoe 140 at 100 micrograms / kg , i.v . - pubmed
significantly blunted the decrease in left ventricular end diastolic pressure but not the decrease in left ventricular systolic pressure after dual NEP - I / ACE - I ( SQ-28603 and enalaprilat each at 30 mumol / kg , i.v . ) - pubmed
This suggests that bradykinin potentiation contributes to the preload - reducing , but not the afterload - reducing , acute effects of NEP - I / ACE - I. - pubmed
Following tyrosine phosphorylation IRS-1 binds to and activates specific proteins containing SH2 domains . - pubmed
Mechanism of enzymic glycoside hydrolysis and of glycosyl transfer by glycosidases and glycosyltransferases . - pubmed
Fluctuations in elastase of cervical mucus and clinical efficacy of cefodizime in obstetric and gynecologic infections . - pubmed
Clinical efficacy of cefodizime ( CDZM ) in obstetric and gynecologic infections and in their prevention , and fluctuations in cervical mucus elastase in gynecologic infections and during pregnancy were studied , and the following results were obtained : 1 . - pubmed
Cervical mucus elastase in uterine and pelvic infections showed high values before administration of CDZM but tended to decline with the progress of cure ( 15/19 cases , 78.9 % ) . - pubmed
In the whole population , there was a statistically significant decrease of PTH , total calcium , and proteins at nearly every time of blood withdrawal , when compared with the preoperative levels . - pubmed
The PTH decreased earlier and total calcium levels were significantly lower after total thyroidectomy than after hemithyroidectomy ( at 48 , 72 , and 96 hours ) . - pubmed
Deficiencies of copper , magnesium , vitamin B6 ( activated by a zinc kinase ) inhibit the conversion of tryptophan to nicotinic acid . - pubmed
This minor form ( AChEII ) was distinguished from the more abundant tetrameric form ( AChEI ) by a higher catalytic subunit relative molecular mass ( M(r ) ) of 80,000 ( 80 K ) , and by a lower affinity for edrophonium - Sepharose . - pubmed
Thus , it is possible that AChEII may be a solubilised form of a dimeric glycosylphosphatidyl inositol - linked AChE. - pubmed
Natural killer ( NK ) cells take up chylomicrons ( CM ) , very low density ( VLDL ) , low density ( LDL ) , high density ( HDL ) and acetyl - modified low density ( AcLDL ) lipoproteins through different receptors , VLDL being the lipoprotein with the highest uptake and HDL the lowest . - pubmed
Although the uptake of lipoproteins assessed by flow cytometry using DiI is not very high , the lipoproteins are able to induce an increase in proliferative responses , VLDL , AcLDL and HDL being the most important ones with 12- and 17 - fold increments , respectively . - pubmed
Incubation of these cells for 48 h with either 13 - cis retinoid acid ( 13 - cis RA ) or all - trans retinoic acid ( ATRA ) resulted in marked inhibition of cell growth , determined by 3H - thymidine incorporation , and in down - regulation of CD25 expression , determined by flow cytometry . - pubmed
pylori cultures contained NADH and NADPH oxidase activity . - pubmed
and the muscarinic antagonist l - methylscopolamine i.c.v . - pubmed
Inhibition of 14 - kDa PLA2 by 2 - acylamino - alkylphospholipids : the influence of amide acidity . - pubmed
2 - Acylamino - alkyl phospholipids are potent competitive inhibitors of 14 - kDa phospholipases A2 ( e.g. - pubmed
As concluded from X - ray studies the amide hydrogen of these inhibitors forms a hydrogen bond to His-48 in the active site of the enzyme . - pubmed
Comparison of isoacidic and isosteric inhibitors reveals that ( i ) the hydrogen bond of the amide proton to His-48 is crucial for strong PLA2 inhibition , ( ii ) regardless of the headgroup unsubstituted N - acyl groups result in optimal amide acidity for PLA2 inhibition and ( iii ) the exceptionally strong inhibition by acetamides and the isosteric fluoroacetamides is due to an additional steric effect . - pubmed
Thirty - one single amino acid substitutions at 24 positions of FLT3L either enhanced or reduced activity in receptor binding and cell proliferation assays . - pubmed
In the present study , the effect of beta - naphthoflavone ( BNF ) , phenobarbital ( PB ) and dexamethasone ( DEX ) on the expression of three cytochrome P450 ( P450 ) isoforms , 1A1 , 2B1 and 3A2 , and on glycogen storage was investigated in intrasplenic liver cell explants in comparison to adult liver . - pubmed
After BNF administration a P450 1A1 expression was induced in the hepatocytes of the peripheral regions of the liver lobules ( zone I , according to Rappaport ) , whereas the staining of the hepatocytes around the central veins disappeared . - pubmed
After BNF treatment a staining for P450 1A1 was induced and also the P450 2B1 expression was slightly more pronounced . - pubmed
PB treatment caused an increase in the staining for P450 2B1 and 3A2 and DEX administration for P450 3A2 within the transplanted hepatocytes . - pubmed
Additionally , after DEX treatment some bile ducts of the explants displayed a slight staining for P450 1A1 , 2B1 and 3A2 . - pubmed
We observed that the amount of Ca2 + taken up by the vesicles increases with the magnitude of the DeltapH across the membrane , particularly at Ca2 + concentrations ( approximately 500 microM ) found optimal for the antiporter activity . - pubmed
Under normal conditions ( H+ pump - induced DeltapH ) , the electrochemical gradient dependence of Ca2 + uptake by the vesicles was checked by inhibition of the process with specific inhibitors ( bafilomycin A ( 1 ) , ergocryptin , folymicin , DCCD ) of the H (+)- pump activity . - pubmed
These results indicate that synaptic vesicles Ca2 +/H+ antiport is indirectly linked to ATP hydrolysis and it is essentially dependent on the chemical component ( DeltapH ) of the electrochemical gradient generated by the H (+)- pump activity . - pubmed
METHODS : Percent fat , fat mass , and fat - free mass ( FFM ) were determined from DXA(QDR ) and DXA(DPX - L ) and from a four - component model based on measures of body density from underwater weighing , body water from deuterium dilution , and BMC from DXA(QDR ) ( 4C(QDR ) ) or DXA(DPX - L ) ( 4C(DPX - L ) ) in young men ( N = 14 ) and women ( N = 10 ) . - pubmed
The topology of the rooted tree and the species distribution of lipocalins suggest that the newly arising lipocalins show a higher rate of amino acid sequence divergence , a higher rate of gene duplication , and their internal pocket has evolved towards binding smaller hydrophobic ligands with more efficiency . - pubmed
A JAK3 - specific inhibitor blocked OSM - stimulated STAT1 tyrosine phosphorylation , DNA - binding activity of STAT1 as well as collagenase-1 ( MMP-1 ) , stromelysin-1 ( MMP-3 ) , collagenase-3 ( MMP-13 ) , and TIMP-3 RNA expression . - pubmed
In contrast , a JAK2 - specific inhibitor , AG490 , had no impact on these events . - pubmed
Similarly , curcumin ( diferuloylmethane ) , an anti - inflammatory agent , suppressed OSM - stimulated STAT1 phosphorylation , DNA - binding activity of STAT1 , and c - Jun N - terminal kinase activation without affecting JAK1 , JAK2 , JAK3 , ERK1 / 2 , and p38 phosphorylation . - pubmed
Curcumin also inhibited OSM - induced MMP-1 , MMP-3 , MMP-13 , and TIMP-3 gene expression . - pubmed
MOZ itself is a 2004 - residue protein containing a putative acetyl CoA - binding motif , so it was hypothesized that MOZ is a HAT . - pubmed
Smaller central pools of cholesterol later in life may contribute to reduced low - density lipoprotein - cholesterol ( LDL - C ) concentrations in the plasma and thus reduce the risk of cardiovascular disease . - pubmed
Both plasma total- and LDL - C were higher ( P & lt;.04 ) in BF compared with RF+cholesterol and RF formula - fed groups at 4 months of age . - pubmed
The recombinant sLDLR was identified by SDS - PAGE , Western blot and Ligand binding blot in supernatant of GS115 / pPIC9K - sLDLr . - pubmed
The SDS - PAGE and Western blot analysis showed that the apparent molecular weight of expressed sLDLR was about 36 kD. - pubmed
Limited proteolysis under catalytic condition ( in presence of PEP , Mg2 + and HCO3 ) although yielded a truncated subunit of 90 kDa , did not affect the catalytic function appreciably but desensitized the enzyme to the effectors like glucose-6 - phosphate glycine and malate . - pubmed
Development of gene therapy using prostate - specific membrane antigen promoter / enhancer with Cre Recombinase / LoxP system for prostate cancer cells under androgen ablation condition . - pubmed
To enhance the efficacy of suicide gene therapy for prostate cancer under androgen deprivation , we designed a promoter system that consists of the prostate - specific membrane antigen ( PSMA ) promoter / enhancer ( PEPM ) and Cre - loxP DNA recombination system . - pubmed
These findings indicate that the combination of the PSMA promoter / enhancer and the Cre - loxP system can enhance the PSMA promoter activity even under androgen ablation conditions and can exert its anti - tumor effect both in vitro and in vivo . - pubmed
Counteracting effects of urea and methylamines in function and structure of skeletal muscle myosin . - pubmed
We have investigated the effects of urea and the methylamines trimethylamine oxide ( TMA - O ) and glycine betaine ( betaine ) on activity and structure of skeletal muscle myosin . - pubmed
K (+ ) EDTA ATPase activity of myosin was almost completely inhibited by urea ( 2 M ) ; TMA - O stimulated myosin activity , whereas betaine had no effect . - pubmed
When combined with urea ( 0 - 2 M ) , TMA - O or betaine ( 1 M ) effectively protected the ATPase activity of myosin against inhibition . - pubmed
Intrinsic fluorescence measurements showed that in urea or TMA - O ( 0 - 2 M ) , there were no shifts in the center of mass of the fluorescence spectrum of myosin , despite a decrease in fluorescence intensity . - pubmed
Both methylamines ( & gt;or=1 M ) were also able to protect myosin structure against urea - induced effects ( 2 - 8 M ) . - pubmed
Alteration of the expression of Bcl-2 , Bcl - x , Bax , Fas , and Fas ligand in the involved skin of psoriasis vulgaris following topical anthralin therapy . - pubmed
In this study , we examined the immunohistochemical expression of apoptosis - regulated protein in the involved psoriatic skin following topical anthralin therapy . - pubmed
Immunohistochemical examination revealed that psoriatic keratinocytes expressed high levels of Bcl - x , which was significantly reduced after anthralin treatment . - pubmed
Bax was not detected in the epidermal keratinocytes in the petrolatum - treated skin , while it was present in the upper keratinocytes after anthralin therapy . - pubmed
Bcl-2 was detected only in basal layers of psoriatic epidermis following both petrolatum and anthralin application . - pubmed
Psoriatic keratinocytes expressed higher levels of Fas in the lower epidermis , while only weak expression was detected in anthralin - treated plaques . - pubmed
Furthermore , anthralin - treated psoriatic epidermis did not express FasL. - pubmed
[ Regulation by ATP of a phospholipase D in the ductal cells of the submaxillary gland ] . - pubmed
Among ATP analogs , BZATP and ATPgammaS were the only ones able to stimulate the PLD activity . - pubmed
PLD stimulation by ATP was inhibited by calphostin C and chelerythrine , two PKC inhibitors . - pubmed
The PLD stimulation by BZ - ATP and TPA , a phorbol ester which activates PKC , were not additive . - pubmed
Inductions of PPARgamma ( 2 ) in WAT and PPAR beta / delta in BAT are blunted by a hypolipemic drug , the ciprofibrate . - pubmed
Unexpectedly , treatment with ciprofibrate deeply affects lipolysis in BAT by increasing acyl - CoA dehydrogenase activity ( 3.4 fold ) and acyl - CoA oxidase at both activity and mRNA levels ( 2.8 and 3.8 fold , respectively ) and enhances strongly UCP1 mRNA level ( 9.5 fold ) during pre - hibernation . - pubmed
The molecularly imprinted polymer ( MIP ) with good selectivity and affinity for ( -)- ephedrine was synthesized with ( -)- ephedrine as the template , methacrylic acid as the functional monomer . - pubmed
This observation supports previous observations , suggesting that the elevation of BNP in cardiac amyloidosis may be due not only to elevated ventricular filling pressure but also to direct myocyte damage due to extracellular deposits of amyloid . - pubmed
MEASUREMENTS : Four measures derived from a stress - strain - coping - support model of alcohol , drugs and gambling problems and the family : Family Member Impact scale ( FMI ) , Symptom Rating Test ( SRT ) , Coping Questionnaire ( CQ ) , and Hopefulness - Hopelessness scale ( HOPE ) . - pubmed
The cupric ion as an inhibitor of photosynthetic electron transport in isolated chloroplasts . - pubmed
Binding of Cu ( 2 + ) to the chloroplast membranes resulted in removal of Cu ( 2 + ) from solution . - pubmed
Therefore , these findings suggest that enhanced NRG1 signaling may contribute to NMDA hypofunction in schizophrenia . - pubmed
Arachidonic acid can function as a signaling modulator by activating the TRPM5 cation channel in taste receptor cells . - pubmed
Intracellular Ca ( 2 + ) is known to be a TRPM5 activator , but the participation of lipid activators remains unreported . - pubmed
To clarify the effect of arachidonic acid on TRPM5 in taste cells , we investigated the expression profile of a series of enzymes involved in controlling the intracellular free arachidonic acid level , with the result that in a subset of taste bud cells , monoglyceride lipase ( MGL ) and cyclooxygenase-2 ( COX-2 ) are expressed as well as the previously reported group IIA phospholipase A ( 2 ) ( PLA ( 2 )- IIA ) . - pubmed
These results strongly suggest the possibility that arachidonic acid acts as a modulator of TRPM5 in taste signaling pathways . - pubmed
Obestatin is a recently discovered 23 amino acids peptide derived from the ghrelin gene . - pubmed
Moreover , obestatin administration had no effects on spontaneous corticosterone secretion . - pubmed
We have developed the computational models to analyze the deleterious nsSNPs in the transporters and predict ones in ABCB ( ATP - binding cassette B ) transporters of interest . - pubmed
EPO effects were similar to those of memantine , a known neuroprotective agent . - pubmed
IOP , in contrast , was unchanged by both EPO and memantine . - pubmed
Blood glucose , serum insulin and adiponectin levels were measured and mRNA for angiotensinogen , angiotensin - converting enzyme and angiotensin II type 1 receptor were determined in adipose tissue samples using RT PCR . - pubmed
Cardiac surgery significantly increased both insulin and blood glucose levels suggesting the development of insulin resistance , while serum adiponectin levels did not change . - pubmed
In hematologic oncology practice , troponin has been investigated extensively as a measure of myocardial injury after therapy with radiation and anthracyclines and after myeloablative therapies with stem cell replacement . - pubmed
IMP preferring cytosolic 5 '- nucleotidase ( cN - II ) is an ubiquitous nucleotide hydrolysing enzyme . - pubmed
19 h later enzyme activity was decreased from 0.7+/-0.026 mU / mg in control ADF cells to 0.45+/-0.046 mU / mg , while cell viability ( evaluated by the MTT reduction assay ) decreased up to 0.59+/-0.01 ( fold vs control ) and caspase 3 activity increased from 136+/-5.8 pmol min(-1 ) mg(-1 ) in control cells to 639+/-37.5 pmol min(-1 ) mg(-1 ) in silenced cells , thus demonstrating that cN - II is essential for cell survival . - pubmed
The results indicate that treatment with xamoterol significantly reduced PKA levels in the retinal endothelial cells cultured in high glucose . - pubmed
Blockade of p42 / p44 or p38 signaling could restore iNOS protein levels to those prior to xamoterol stimulation , suggesting that beta - adrenergic receptor regulates iNOS protein levels through regulation of PKA and MAP kinase signaling . - pubmed
Using a copper sheet of 2.0 mm thickness or more prevented over - sensing of pacemakers in the present study , especially when serial X - ray exposures were used with an over - table fluoroscopy system or cone - beam CT unit . - pubmed
MAIN OUTCOME MEASURE(S ): Glucose , triglycerides , high - density lipoprotein cholesterol , low - density lipoprotein cholesterol , DHEAS , total T , insulin , homeostatic model assessment for insulin resistance ( HOMA - IR ) , and visfatin levels were measured . - pubmed
After therapy , statistically significant decreases in HOMA - IR , fasting insulin , free T , and DHEAS concentrations were observed for the PCOS group . - pubmed
Nicotine- and methamphetamine - induced dopamine release evaluated with in - vivo binding of radiolabelled raclopride to dopamine D2 receptors : comparison with in - vivo microdialysis data . - pubmed
The effect of substances which alter extracellular dopamine ( DA ) concentration has been studied by measuring changes in the binding of radiolabelled raclopride , a DA D2 receptor ligand that is sensitive to endogenous DA . - pubmed
The apparent discrepancy in the extrasynaptic DA -[ 3H ] raclopride binding relationship between the DA - enhancing drugs with and without DAT - blocking property indicates that the competition between endogenous DA and radiolabelled raclopride takes place at the intrasynaptic rather than extrasynaptic DA D2 receptors and reflects synaptic concentration of DA . - pubmed
In this study we identified the GSK3 beta N - terminal amino acids 78 - 92 as necessary for its association with p53 . - pubmed
Inhibitors of GSK3 impaired the acetylation of p53 at Lys373 and Lys382 near the GSK3 beta binding region in p53 , indicating that GSK3 beta facilitates p53 acetylation . - pubmed
Cephalosporins , aminoglycosides ( usually IV gentamicin ) , IV amoxicillin , and once co - trimoxazole were recommended . - pubmed
These results suggested that the timing of CBT with RIC may be very important , and cytoreduction with not only ASCT but also bortezomib could give a promising chance for a successful CBT . - pubmed
Inhibition of oral midazolam clearance by boosting doses of ritonavir , and by 4,4 - dimethyl - benziso -( 2H )- selenazine ( ALT-2074 ) , an experimental catalytic mimic of glutathione oxidase . - pubmed
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT : * The viral protease inhibitor ritonavir is known to inhibit clearance of intravenous midazolam . - pubmed
* Therefore ritonavir is an appropriate positive control inhibitor for clinical drug interaction studies involving CYP3A substrates . - pubmed
AIMS : We evaluated whether ' boosting ' doses of ritonavir can serve as a positive control inhibitor for pharmacokinetic drug - drug interaction studies involving cytochrome P450 3A ( CYP3A ) . - pubmed
The study also determined whether 4,4 - dimethyl - benziso -( 2H )- selenazine ( ALT-2074 ) , an investigational organoselenium compound that acts as a catalytic mimic of glutathione oxidase , inhibits CYP3A metabolism in vivo . - pubmed
CONCLUSIONS : Low - dose ritonavir produces extensive CYP3A inhibition exceeding that of ketoconazole ( typically 10- to 15 - fold midazolam AUC enhancement ) , and is a suitable positive control index inhibitor for drug - drug interaction studies . - pubmed
ALT-2074 inhibits CYP3A metabolism to a small degree that is of uncertain clinical importance . - pubmed
In addition , three flavonoids ( kaempferol 3 - O - glycoside , apigenin-7 - O - rutinoside , and naringenin ) were identified by using an LC - MS / MS apparatus . - pubmed
MS2 titers were reduced by 1.14 log(10 ) PFU / ml with CJ , and 0.55 , 0.80 , and 0.96 log(10 ) PFU / ml with 0.15 , 0.30 , and 0.60 mg / ml PAC , respectively . - pubmed
INTERVENTION : We report 2 cases in which n - BCA was used effectively as a salvage rescue in aneurysms that ruptured during coil embolization . - pubmed
Finally a method for the surface treatment of Sylgard((R ) ) using sulphuric acid and fibronectin to enhance smooth muscle cell ( SMC ) adhesion is proposed and examined in vitro . - pubmed
We compared the effects of letrozole with that of oxandrolone on predicted adult height ( PAH ) , puberty , bone mineral density , serum insulin - like growth factor 1 ( IGF-1 ) and blood lipoproteins . - pubmed
RESULTS : Letrozole differed from oxandrolone and placebo in significantly increasing PAH ( p & lt ; 0.05 ) , and slightly but significantly decreasing HDL - cholesterol . - pubmed
The G6PD enzyme catalyzes the first step in the pentose phosphate pathway to protect cells against oxidative stress . - pubmed
Replacing the serine residue S621 of the SGK consensus motif by an alanine ( S621A ) abolished this stimulatory effect . - pubmed
Following oxidative stress , DEDC sequentially decreased mitochondrial membrane potential ( DeltaPsim ) , reduced Bcl-2 expression , increased cytochrome c release , and activated caspase-3 , - 8 , and - 9 . - pubmed
Phosphorylation of c - Jun N - terminal kinase ( JNK ) and p38 mitogen activated protein kinase ( MAPK ) was stimulated by treatment with DEDC . - pubmed
To further investigate the mechanisms of the DEDC - induced cell death , we examined the effects of reactive oxygen species scavenger N - acetyl - L - cysteine ( NAC ) and selective inhibitors for MAPK pathways on the cell death . - pubmed
The DEDC - induced cell death was significantly inhibited by both NAC and JNK inhibitor SP600125 , but promoted by p38 MAPK inhibitor , SB203580 . - pubmed
Bcl - x ( L ) blocks a mitochondrial inner membrane channel and prevents Ca2 + overload - mediated cell death . - pubmed
We have found that Bcl - x ( L ) neuroprotective actions take place at mitochondria where this antiapoptotic protein delays both mitochondrial potential collapse and opening of the permeability transition pore by preventing Ca ( 2 +)-mediated mitochondrial multiple conductance channel opening . - pubmed
Bcl - x ( L ) neuroprotective actions were antagonized by the Bcl - x ( L ) inhibitor ABT-737 and potentiated by the Ca ( 2 + ) chelator BAPTA - AM . - pubmed
The following general trends were observed : ( i ) order - order transitions from HEX Gyroid ( G ) Lamellae ( LAM ) ; ( ii ) the inter - cylinder spacing of the HEX phase increased ; ( iii ) when the homopolymer molecular weight and concentration was high , macrophase separation of the system occurred . - pubmed
A multiwalled carbon nanotube ( MWCNT ) modified glassy carbon electrode ( GCE ) is used for the simultaneous determination of cefpirome by differential pulse voltammetry and square wave voltammetry . - pubmed
Results indicated that cathodic peak of cefpirome is greatly improved at MWCNT modified GC electrode as compared with the bare GC electrode showing excellent electrocatalytic activity towards cefpirome reduction . - pubmed
Low folate reduced overall methylation of Slc394a by 3.4 % ( p=0.038 ) but did not affect Esr1 or Igf2 differentially methylated region ( DMR ) 1 . - pubmed
Plasma lipids were not different between Strongmen and sedentary controls , but in comparison to runners , Strongmen had higher low - density lipoprotein - cholesterol ( p & lt ; 0.05 ) and lower high - density lipoprotein cholesterol ( p & lt ; 0.01 ) . - pubmed
The recent identification of a fourth histamine receptor has reinforced clinical interest in the pleiotropic effects of histamine and the relative roles of histamine receptors in mediating immune and inflammatory responses . - pubmed
MATERIAL AND METHODS : A comprehensive literature search was conducted for the following terms , alone or in combination : allergic rhinitis , asthma , antihistamines , histamine , and histamine receptors , and for the second - generation antihistamines azelastine , cetirizine , desloratadine , ebastine , fexofenadine , levocetirizine , loratadine , mizolastine , and rupatadine . - pubmed
RESULTS : The study did not find a significant association between alcohol - use disorders and HRQoL [ Physical Component Summary ( PCS ) and Mental health Component Summary ( MCS ) ] in this clinic population . - pubmed
Exhaled nitric oxide ( FeNO ) has been associated with bronchial eosinophilia and with airway hyperresponsiveness ( AHR ) in mild stable asthma . - pubmed
We previously demonstrated in a large project that allergen exposure is able to raise FeNO and to worsen AHR to bradykinin . - pubmed
We postulated that allergen - induced increase in FeNO could be related to heightened mucosal eosinophils and AHR to bradykinin in atopic asthma . - pubmed
Inflammatory cell counts were related to FeNO and AHR to BK ( expressed as logPD20 bradykinin ) . - pubmed
UNLABELLED : We studied the metabolic characteristics of RCC subtypes and angiomyolipoma with 18F - FDG and 11C - acetate PET / CT . - pubmed
Each lesion was assessed for SUVmax ratio ( lesion - to - normal kidney ) on 11C - acetate/18F - FDG PET and attenuation density on CT . - pubmed
CONCLUSION : 11C - acetate PET / CT helps in differentiating " fat - poor angiomyolipoma " from RCC . - pubmed
RESULTS : There were no statistical differences concerning age , disease duration , L - Dopa equivalent dose , disease severity ( UPDRS III ) or dopaminergic uptake in FP - CIT - SPECT at baseline between both subgroups . - pubmed
Using cell imaging , proteomics , and lipidomics , we show that sFLT1 binds to the glycosphingolipid GM3 in lipid rafts on the surface of podocytes , promoting adhesion and rapid actin reorganization . - pubmed
The solvent - free melt electrospinning ( MES ) method was developed to prepare a drug delivery system with fast release of carvedilol ( CAR ) , a drug with poor water solubility . - pubmed
The nature of cell death was assessed by doubles - taining cells with fluorescein isothiocyanate - labeled Annexin V and propidium iodide , and then analyzing the cells using flow cytometry . - pubmed
Pretreatment with propofol suppressed H ( 2 ) O ( 2 )-induced LDH release in a concentration - dependent manner , reducing the percentage of LDH release to 38.1 + - 5.6 % , 33.5 + - 6.3 % , and 26.2 + - 3.8 % of the controls at 10 microM , 25 microM and 50 microM propofol , respectively . - pubmed
Numbers of viable cells increased following propofol pretreatment , with 11.4 + - 10.9 % , 19.5 + - 16.1 % , and 32.4 + - 23.3 % cell survival rates after pretreatment with 10 microM , 25 microM and 50 microM propofol , respectively . - pubmed
These substitutions control hole delocalization in the QDs through the energetic alignment of the highest occupied orbitals of PTC with the highest density - of - states region of the CdSe valence band , to which PTC couples selectively . - pubmed
Antiplatelet therapy with aspirin and a platelet P2Y12 receptor antagonist reduces thrombotic and ischemic events after percutaneous coronary intervention and acute coronary syndrome . - pubmed
Radiosensitization of prostate cancer cells by the dual PI3K / mTOR inhibitor BEZ235 under normoxic and hypoxic conditions . - pubmed
We investigated whether the novel PI3K / mTOR inhibitor BEZ235 might sensitize prostate cancer cells to radiation and reduce hypoxia - induced radioresistance . - pubmed
RESULTS : BEZ235 rapidly inhibited PI3 K and mTOR signaling in a dose dependent manner and limited tumor cell proliferation and clonogenic survival in both cell lines independently of PTEN status . - pubmed
Moreover , BEZ235 inhibited PI3K / mTOR activation and radiosensitized both cell lines under normoxia and hypoxia . - pubmed
BEZ235 radiosensitizing effects correlated with a decrease in gammaH2AX foci repair and increased G2 / M cell cycle arrest . - pubmed
Metformin also demonstrated a significant between - group advantage for BMI ( -0.7 ; 95 % CI=-1.1 to -0.2 ) , triglyceride level ( -20.2 mg / dL ; 95 % CI=-39.2 to -1.3 ) , and hemoglobin A1c level ( -0.07 % ; 95 % CI=-0.14 to -0.004 ) . - pubmed
AIM : To assess whole - body fluorodeoxyglucose ( FDG ) positron emission tomography / computed tomography ( PET / CT ) in the management of small bowel obstructions ( SBOs ) secondary to gastric cancer and its role in treatment strategies . - pubmed
Intravenous paracetamol was found to be the most effective in reducing both blood pressure and heart rate ( p & lt ; 0.001).The effect on post - operative pain was insignificant . - pubmed
By using interactive essential dynamics analysis , we found that Galphas and Gbetagamma domain of Gs protein had the tendency to separate when the inverse agonist ICI 118,551 bound to beta2AR . - pubmed
The alpha5 - helix had a relatively quick movement with respect to transmembrane segments of beta2AR when the inverse agonist ICI 118,551 bound to beta2AR . - pubmed
The present study focuses on the role of chitosan ( CS ) as nanofiller of graphite oxide ( GO ) in order to prepare composite materials with improved Hg ( II ) adsorption properties . - pubmed
The removal of Hg ( II ) from aqueous solutions was studied using adsorbents as graphite oxide ( GO ) , graphite oxide nanofilled with chitosan ( GO / CS ) and magnetic chitosan ( GO / mCS ) . - pubmed
The zonation and structure of phototrophic microbial mats were studied along two thermal gradients in sulfide - rich hot springs of southwest Iceland . - pubmed
Bisulfite sequencing analysis revealed the premature promoter demethylation of Gata1 , a key myeloerythroid transcription factor and a PIAS1 - target gene , in Pias1(-/- ) HSCs . - pubmed
He had a positive In - pentetreotide indicating the presence of somatostatin receptors . - pubmed
H2O2 increased the density of nine spots on two - dimensional gel electrophoresis of anti - GSK-3beta - immunoprecipitates by more than 3 - fold . - pubmed
The mitochondrial translocation of GSK-3beta was attenuated also when Lys-15 , but not Arg-4 or Arg-6 , in the N - terminal domain of GSK-3beta was replaced with alanine . - pubmed
The oxidative stress - induced mitochondrial translocation of GSK-3beta was associated with an increase in cell death , which was suppressed by lithium chloride ( LiCl ) , a GSK-3beta inhibitor . - pubmed
Comprehensive profiling of the reaction of adenine propenal with human serum albumin and the DNA repair protein , XPA , revealed that the only stable adduct is N ( epsilon )- oxopropenyllysine . - pubmed
Modification by adenine propenal dramatically reduces XPA 's ability to bind to a DNA substrate . - pubmed
The GAD and GABA - T enzyme activities were determined by measuring GABA and NADH production , respectively . - pubmed
The GAD enzyme inhibitor semicarbazide ( SCZ ) blocked the sedative and hypnotic effects of B2 . - pubmed
Multicentre randomised phase II trial of gemcitabine+platinum , with or without trastuzumab , in advanced or metastatic urothelial carcinoma overexpressing Her2 . - pubmed
CONCLUSION : The unexpectedly low incidence of Her2 overexpression precluded the detection of a significant difference in efficacy on addition of trastuzumab to platinum - based chemotherapy with gemcitabine . - pubmed
Bosentan exhibited a positive exposure - adjusted signal in one assay ( BSEP inhibition ) and a moderate CVB body burden . - pubmed
coli strain , harbouring a gene cassette ( camA ( + ) camB ( + ) camC ) that encodes the F87W / Y96F / L244A / V247L mutant of cytochrome P-450cam ( CYP101 ) , oxidised HCB to PCP . - pubmed
P2X7 R - mediated Ca ( 2 + ) -independent d - serine release via pannexin-1 of the P2X7 R - pannexin-1 complex in astrocytes . - pubmed
D - serine is a coagonist of N - methyl - d - aspartate ( NMDA ) subtype of glutamate receptor and plays a role in regulating activity - dependent synaptic plasticity . - pubmed
In this study , we examined the mechanism by which extracellular ATP triggers the release of d - serine from astrocytes and discovered a novel Ca ( 2 + ) -independent release mechanism mediated by P2X7 receptors ( P2X7 R ) . - pubmed
Using [ ( 3 ) H ] d - serine , which was loaded into astrocytes via the neutral amino acid transporter 2 ( ASCT2 ) , we observed that ATP and a potent P2X7 R agonist , 2 '( 3 ')- O -( 4 - benzoylbenzoyl ) adenosine-5 '- triphosphate ( BzATP ) , stimulated [ ( 3 ) H ] D - serine release and that were abolished by P2X7 R selective antagonists and by shRNAs , whereas enhanced by removal of intracellular or extracellular Ca ( 2 + ) . - pubmed
The P2X7 R - mediated d - serine release was inhibited by pannexin-1 antagonists , such as carbenoxolone ( CBX ) , probenecid ( PBN ) , and ( 10 ) Panx-1 peptide , and shRNAs , and stimulation of P2X7 R induced P2X7 R - pannexin-1 complex formation . - pubmed
Simply incubating astrocytes in Ca ( 2 + ) /Mg ( 2 + ) -free buffer also induced the complex formation , and that enhanced basal d - serine release through pannexin-1 . - pubmed
The P2X7 R - mediated d - serine release assayed in Ca ( 2 + ) /Mg ( 2 + ) -free buffer was enhanced as well , and that was inhibited by CBX . - pubmed
Treating astrocytes with general protein kinase C ( PKC ) inhibitors , such as chelerythrine , GF109203X , and staurosporine , but not Ca ( 2 + ) -dependent PKC inhibitor , Go6976 , inhibited the P2X7 R - mediated d - serine release . - pubmed
Thus , we conclude that in astrocytes , P2X7 R - pannexin-1 complex formation is crucial for P2X7 R - mediated d - serine release through pannexin-1 hemichannel . - pubmed
The release is Ca ( 2 + ) -independent and regulates by a Ca ( 2 + ) -independent PKC . - pubmed
The activated P2X7 R per se is also functioned as a permeation channel to release d - serine in part . - pubmed
This P2X7 R - mediated d - serine release represents an important mechanism for activity - dependent neuron - glia interaction . - pubmed
METHODS : Peripheral blood leukocyte and lymphocyte subsets , including CD3(+ ) , CD4(+ ) , and CD8(+ ) T cells ; CD19(+ ) B cells ; and CD56(+ ) natural killer ( NK ) cells , were obtained at baseline and monitored at 3 months , 6 months , and 12 months after initiation of DMF treatment . - pubmed
Employing this methodology , a number of acylpolyamine spider toxins were synthesized and identified as venom components by UHPLC and ESI - MS / MS . - pubmed
OBJECTIVE : To evaluate effects of holding parenteral nutrition ( PN ) for 3 hours prior to newborn screening ( NBS ) on false - positive NBS rate for amino acids ( AAs ) in very low birth weight ( VLBW ) infants ( birth weight & lt;1500 g ) . - pubmed
OBJECTIVE : In this study , a pilot - scale investigation was conducted to examine and compare the biotoxicity of the organic compounds in effluents from five treatment processes ( P1 - P5 ) where each process was combination of preoxidation ( O3 ) , coagulation , sedimentation , sand filtration , ozonation , granular activated carbon , biological activated carbon and chlorination ( NaClO ) . - pubmed
Glycosylation inhibition reduces cholesterol accumulation in NPC1 protein - deficient cells . - pubmed
We tested the hypothesis that Niemann - Pick type C 1 ( NPC1 ) protein aids the transfer of low density lipoprotein - derived cholesterol across this glycocalyx . - pubmed
CRISPR generated , NPC1 - deficient ldl - D cells supplemented with galactose accumulated more cholesterol than those in which sugar addition was blocked . - pubmed
In the absence of galactose supplementation , NPC1 - deficient ldl - D cells also transported more cholesterol from lysosomes to the endoplasmic reticulum , as monitored by an increase in cholesteryl [ ( 14 ) C ]- oleate levels . - pubmed
These experiments support a model in which NPC1 protein functions to transfer cholesterol past a lysosomal glycocalyx . - pubmed
These four compounds showed significant Ebola activity that is not specifically due to their inhibition of S - adenonosylhomocysteine hydrolase , as might have been expected for 3 - deazaadenine carbocyclic nucleosides . - pubmed
Synthesis , characterization , and H / D exchange of mu - hydride - containing [ FeFe]-hydrogenase subsite models formed by protonation reactions of ( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 ( TDT = SCH2SCH2S ) with protic acids . - pubmed
As [ FeFe ]- hydrogenase models , the first thiodithiolate ( TDT ) ligand - containing mu - hydride complexes [ ( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 ( mu - H )](+) Y (- ) ( 2 - 7 , Y = Cl , ClO4 , PF6 , BF4 , CF3CO2 , CF3SO3 ) have been prepared by protonation reactions of ( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 ( 1 ) with the corresponding HY acids . - pubmed
Cystatin C- and creatinine - based estimation of glomerular filtration rate ( eGFRcys and eGRFcreat , respectively ) and urinary albumin / creatinine ratio ( ACR ) were assessed to investigate their relationship with the risk of atrial fibrillation . - pubmed
Transient receptor potential ( TRP ) channels are recently identified proteins that form a versatile family of ion channels , the majority of which are calcium permeable and exhibit complex regulatory patterns with sensitivity to multiple environmental factors . - pubmed
RESULTS : Statins , proton pump inhibitors , angiotensin - converting enzyme inhibitors , and selective serotonin reuptake inhibitors were determined to have class equivalence for efficacy , toxicity , and convenience . - pubmed
Leuprolide acetate , a synthetic nonapeptide analogue of gonadotropin - releasing hormone ( GnRH or LHRH ) , is the active pharmaceutical ingredient used to treat a wide range of sex hormone - related disorders , including advanced prostatic cancer , endometriosis , and precocious puberty . - pubmed
Association between lipoxin A4 and interleukin-12 in gingival crevicular fluid : a preliminary investigation . - pubmed
This study aimed to explore the possibility of an association between lipoxin A4 and interleukin-12 in chronic periodontitis . - pubmed
Gingival crevicular fluid samples were collected and analysed for interleukin-12 and lipoxin A4 using ELISA . - pubmed
There was a negative correlation between interleukin-12 and lipoxin A4 in both groups . - pubmed
There was a negative correlation between clinical attachment loss and lipoxin A4 , and a positive correlation between clinical attachment loss and interleukin-12 . - pubmed
Lipoxin A4 possibly has an inhibitory effect on interleukin-12 . - pubmed
An FDG PET / CT scan was performed to evaluate the whole body in November 2015 . - pubmed
MATERIALS AND METHODS : The SRP 68 01 gamma spectrometer with attached scintillation detector based on a NaI ( Tl ) O 30 x 25 mm2 crystal and NC 482 VM one channel spectrometer ( Bicron Co. ) with attached scintillation detector based on a NaI ( Tl ) O 52 x 52 mm2 crystal were used for the study . - pubmed
It Remains Unknown Whether Filaggrin Gene Mutations Evolved to Increase Cutaneous Synthesis of Vitamin D. About 8 - 10 % of normal Northern Europeans are heterozygous carriers of common FLG mutations , while only 1 - 4 % of southern Europeans display these mutations , and only very rarely are mutations detected in African populations . - pubmed
Importantly , FLG mutation carriers have 10 % increased serum vitamin D concentrations compared to controls . - pubmed
Based on these observations , we have proposed that this latitude - dependent gradient of FLG mutations across Europe , Asia and Africa could have provided an evolutionary advantage for heterozygous FLG mutation carriers , residing at northern latitudes , depletion of the FLG downstream product , trans - urocanic acid , would facilitate the intracutaneous synthesis of vitamin D3 by allowing increased transcutaneous absorption of UVB photons . - pubmed
Proteome analysis of irradiated endothelial cells reveals persistent alteration in protein degradation and the RhoGDI and NO signalling pathways . - pubmed
RESULTS : The radiation - induced alteration of the endothelial proteome was characterized by sustained perturbation of Rho GDP - dissociation inhibitor ( RhoGDI ) and nitric oxide ( NO ) signalling pathways . - pubmed
The cumulative number of alleles associated with lower DHEAS within genes ZKSCAN5 , SULT2A1 , HHEX , and ARPC1A present in each individual negatively correlated with DHEAS levels in RA patients , but not in controls . - pubmed
Linear regression analysis showed significant effect of polymorphisms in genes ZKSCAN5 and ARPC1A on serum DHEAS levels in female RA patients but not in the control group . - pubmed
Urate promotes SNCA / alpha - synuclein clearance via regulating mTOR - dependent macroautophagy . - pubmed
Moreover , UA was still able to increase the LC3 - II level and the number of LC3 puncta in the presence of Bafilomycin A1 , a lysosomal inhibitor . - pubmed
Co - treatment with 3 - benzyl-5 -(( 2 - nitrophenoxy ) methyl )- dihydrofuran-2 ( 3H )- one ( 3BDO ) , an mTOR activator , abolished the UA - induced LC3 - II increase . - pubmed
METHODS : A total of 157 HIV - infected patients were screened through standard serological tests , including Wright 's test , Coombs ' Wright test , and 2 - mercaptoethanol Brucella agglutination test ( 2ME test ) , blood cultures in Castaneda media , and CD4 counting . - pubmed
Furthermore , the LsEm1 gene was also expressed in response to salt , dehydration , abscisic acid ( ABA ) , and cold stresses in young seedlings . - pubmed
ALRN-6924 , a stapled peptide that blocks interactions between p53 and both MDM2 and MDMX has potent in vitro activity and superior in vivo activity across 8 different PDX models compared to the standard - of - care agent romidepsin . - pubmed
We prepared the photosensitizer conjugates by expressing a recombinant Fab fragment and specifically linking IR700 - maleimide at the C - terminal of the Fab heavy chain . - pubmed
Exogenous application of the ethylene precursor 1 - aminocyclopropane-1 - carboxylic acid ( ACC ) was found to promote the emergence of nodal roots . - pubmed
Transcriptome analysis of nodal tissues revealed that the expression of genes involved in metabolic processes and cell wall biogenesis was upregulated in response to ACC treatment , supporting the notion that ethylene is a positive regulator for the outgrowth of young root primordia . - pubmed
In BSV::ARGOS8 transgenic plants with reduced ethylene sensitivity due to constitutive overexpression of ARGOS8 , nodal root emergence was delayed and the promotional effect of ACC on nodal root emergence decreased . - pubmed
This finding reveals how GARP exploits an unusual medley of interactions , including fold complementation by the amino terminus of TGF - beta1 , to chaperone and orient the cytokine for binding and activation by alphaVbeta8 . - pubmed
The electrical performance of the grown NiSi / SiC core - shell nanowires was characterized by the conducting AFM and it is found that the measured conductivities of the nanowires were higher than the reported works that might be enhanced by SiC shell layer on NiSi nanowires . - pubmed
Copanlisib : An Intravenous Phosphatidylinositol 3 - Kinase ( PI3 K ) Inhibitor for the Treatment of Relapsed Follicular Lymphoma . - pubmed
Data Sources : Sources of information were identified through searches of PubMed ( August 2014 to January 2019 ) using the key terms copanlisib , Aliqopa , PI3 K inhibitor , and BAY 80 - 6946 . - pubmed
The safety and efficacy of copanlisib has been studied in the multicenter , single - arm , phase II CHRONOS-1 study . - pubmed
Angiotensin II ( ATII ) is a noncatecholamine vasopressor recently approved for the management of distributive shock , but limited data support its clinical utility . - pubmed
To evaluate bone metabolism , markers ( alkaline phosphatase and parathyroid hormone - PTH ) and related nutrients ( vitamin D , vitamin B12 , calcium , phosphorus , magnesium , potassium and zinc ) were analyzed . - pubmed
After logistic regression , it was observed that inadequacies of calcium ( OR : 4.11 ; 95 % CI : 2.33 - 6.66 ) , phosphorus ( OR : 3.03 ; 95 % CI : 1.98 - 5.79 ) , vitamin D ( OR : 5.01 ; 95 % CI : 2.92 - 6.71 ) and PTH ( OR : 5.45 ; 95 % CI : 4.49 - 6.74 ) were significantly higher in the MUHO group compared to the MHO Group . - pubmed
Following resonance - enhanced multiphoton ionisation via the 11pipi * and 11pisigma * states of phenol , we observe 11pipi*-D0 / D1 ionisation and competing direct S0 - D0 / D1 ionisation . - pubmed
We also present a computational strategy for calculating vertical ionisation energies using a quantum - mechanics / effective fragmentation potential ( QM / EFP ) approach , in which we find that optimising the configurations obtained from molecular dynamics simulations and using the [ phenol ( H2O ) 5 ] QM [( H2O ) n>=250]EFP ( B3LYP / aug - cc - pvdz ) method gives good agreement with experiment . - pubmed
A composite chitosan / nano - activated carbon ( CS - NAC ) aminated by ( 3 - aminopropyl ) triethoxysilane ( APTES ) was prepared in the form of beads and applied for the removal of acetaminophen from aqueous solutions . - pubmed
The aminated adsorbent ( CS - NAC - APTES beads ) prepared with 40 % w / w NAC and 2 % v / v APTES showed higher adsorption capacity ( 407.83 mg / g ) than CS - NAC beads ( 278.4 mg / g ) . - pubmed
Brunauer - Emmett - Teller ( BET ) analysis demonstrated that the surface area of the CS - NAC - APTES beads was larger than that of CS - NAC beads ( 1.16 times ) . - pubmed
To demonstrate applications of this CRISPR - dCpf1 system in metabolic engineering , we applied this system to repress four genes involved in lysine biosynthesis ( gltA , pck , pgi , and hom ) with a single array , which increased the lysine titer and yield for over 4.0 - fold . - pubmed
Fucoidan , a ligand for the homing molecule l - selectin ( CD62L ) , is known to induce rapid up - regulation of several chemokine receptors on lymphocytes . - pubmed
Transcriptional Induction of Cystathionine gamma - Lyase , a Reactive Sulfur - Producing Enzyme , by Copper Diethyldithiocarbamate in Cultured Vascular Endothelial Cells . - pubmed
Reactive sulfur species are produced by enzymes , such as cystathionine gamma - lyase ( CSE ) , cystathionine beta - synthase , 3 - mercaptopyruvate sulfurtransferase , and cysteinyl - tRNA synthetase . - pubmed
Cu10 selectively upregulated CSE gene expression in vascular endothelial cells independent of cell density . - pubmed
This transcriptional induction of endothelial CSE required both the diethyldithiocarbamate scaffold and the coordinated copper ion . - pubmed
Additionally , the present study revealed that ERK1 / 2 , p38 MAPK , and hypoxia - inducible factor ( HIF )- 1alpha / HIF-1beta pathways mediate transcriptional induction of endothelial CSE by Cu10 . - pubmed
The transcription factors NF - kappaB , Sp1 , and ATF4 were suggested to act in constitutive CSE expression , although the possibility that they are involved in the CSE induction by Cu10 can not be excluded . - pubmed
The present study used a copper complex as a molecular probe to reveal that the transcription of CSE is regulated by multiple pathways in vascular endothelial cells , including ERK1 / 2 , p38 MAPK , and HIF-1alpha / HIF-1beta . - pubmed
Certain antiviral drugs such as Remdesivir are RNA dependent RNA polymerase inhibitor . - pubmed
RESULTS : It is reported that ATP is involved in synthesis of coronavirus non - structural proteins from 3CLpro and PLpro . - pubmed
Protein adsorption studies showed the PEUs did not led to significant platelet adsorption in platelet rich plasma ( PRP ) after pretreatment with fibrinogen . - pubmed
Release of human chorionic somatomammotrophin from isolated perfused lobules and superfused fragments of term placenta : spontaneous liberation and the effects of cholinergic drugs , dibutyrylcyclic adenosine monophosphate and calcium . - pubmed
Single isolated lobules from term placentae were bilaterally perfused with Earle 's solution , and the release of human chorionic somatomammotrophin ( HCS ) was measured by radioimmunoassay . - pubmed
The ability of the placenta to respond to alterations in the perfusion environment was verified by stimulating the HCS secretion with 1 mM dibutyryl cyclic adenosine monophosphate and by modifying calcium concentrations . - pubmed
High calcium ( 10 mM CaCl2 ) led to a sustained increase of HCS release . - pubmed
Three criteria of enzyme activity were compared : A , total radioactivity of the washed protein precipitate ; B , this figure minus activity incorporated in the absence of the collagen acceptor ; and C , radioactivity incorporated into the mixed amino acid fraction , collected by elution with dilute NH4OH from a Dowex 50 resin column after alkaline hydrolysis of the protein . - pubmed
The ratio of EGOT with in vitro stimulation by PLP to EGOT actual ( alpha - EGOT ) was also studied . - pubmed
Prostaglandin E2 has previously been shown to enhance the shape transformation of sickle prone erythrocytes ( 8) and to reduce the oxygen resaturation of Hemoglobin SS within intact sickle cell erythrocytes after deoxygenation ( 15 ) . - pubmed
The involvement of active oxygen species , such as singlet oxygen and H2O2 , was established by the inhibition of DNA breakage by catalase and by sodium azide . - pubmed
To elucidate a possible mechanism for the inhibition of AS101 , we have analyzed the effect of the drug on the catalytic functions associated with HIV RT , namely the RDDP , DDDP , and RNase H activities . - pubmed
On the other hand , the RNase H activity was less sensitive to AS101 , with an apparent IC50 value of about 30 microM. - pubmed
The ability of IL-10 and TGF - beta to suppress microbicidal function was also positively correlated with inhibition of nitrite generation in macrophage culture supernatants . - pubmed
These and the deduced amino acid sequences were compared with those of a subtype O1 virus strain . - pubmed
VP4 and VP2 differed by 2.4 and 1.8 % , whereas VP1 , known as major viral antigen , and VP3 differed by 8 % and 5.5 % , respectively . - pubmed
The glucose - phosphorylating enzyme glucokinase likely plays an important role in regulating glucose - stimulated insulin secretion from the islets of Langerhans and has previously been thought to be expressed only in that tissue and in liver . - pubmed
AtT20ins cells , in contrast , exhibited no response to glucose at any concentration tested , despite the fact that insulin secretion from both cell lines was stimulated by incubation with dibutyryl cAMP . - pubmed
The intensity of the TMAO resonance for CRF subjects was correlated with the plasma concentration of urea ( R = 0.55 ) and creatinine ( R = 0.74 ) , suggesting that the presence of TMAO is closely related to the degree of renal failure . - pubmed
Among individual phenomena the following are important : in stage I regular contact with the drug ( 04 ) and increased tolerance ( 05 ) , in stage II secret drinking ( 2 ) and frequency of palimpsests ( 7 ) , in stage III signs derived mostly from rationalization and alcohol - centered behaviour and finally in stage IV impaired thinking ( 33 ) , reduced tolerance ( 37 ) and possible ethic degradation ( 32 ) . - pubmed
The level of glucokinase activity does not appear to be responsible for poor glucose utilization in liver . - pubmed
The binding of palmitate to the protein was saturable . - pubmed
In contrast , the activity of His - tRNA synthetase was significantly increased in the symptomatic phase of MeHg intoxication . - pubmed
The activities of these six aminoacyl - tRNA synthetases in the control brains were affected to different extents on the direct addition of MeHg to the assay system in vitro . - pubmed
The chief advantages over conventional methods include virtually no loss of leukocytes during washing and separation steps ; a significant reduction in the time required to prepare leukocytes for radiolabeling compared to non - hemolytic preparations ; a 28 % increase in the average labeling efficiency obtained using 111In - oxine ; greater than 95 % cell viability as measured by the trypan blue exclusion test ; elimination of contaminating red blood cells from the leukocyte pellet prior to labeling ; and 80 % survivability at 15 min post injection ( measured as per cent of blood activity on leukocyte fraction ) . - pubmed
Inhibition of myogenesis by 5 - bromodeoxyuridine or by calcium deprivation prevents the developmentally associated changes in the expression of H36 . - pubmed
We conclude that H36 is a muscle - specific , developmentally regulated cell - surface antigen that may have a role in myoblast differentiation and that can be used to determine the embryonic lineages of skeletal and cardiac muscle . - pubmed
IRBP thus appears to be a unique , conserved glycoprotein of the retinal extracellular matrix that could serve as a retinoid - transport vehicle . - pubmed
The influence of triamcinolone upon the content of antidiuretic hormone in the hypothalamus and its release from the hypophysis into blood . - pubmed
Increased release of LH after administration of LH - RH to men pretreated with clomiphene . - pubmed
Among the various cell types , the extent of [ 3H ] PDBu binding did not correlate with the extent of EGF binding , indicating that these two substances occupy distinctly separate types of receptors . - pubmed
If present in sufficient amounts , aromatase could catalyze the synthesis of estrogens from plasma steroid precursors and produce high breast cancer tissue concentrations . - pubmed
To determine the biological importance of tumor aromatase , we validated a specific and highly sensitive 3H - labeled water release assay for aromatase and used this to quantitate the amount of estrogen synthesized in vitro in breast tumors . - pubmed
h. Three aromatase inhibitors ( aminoglutethimide , testololactone , and 4 - hydroxyandrostenedione ) blocked this activity at concentrations similar to those affecting aromatase activity in other tissues . - pubmed
If biologically important , the estrogen formed locally from aromatase would be expected to stimulate production of the progesterone receptor . - pubmed
Under these circumstances , a positive correlation of progesterone receptor and local estrogen production should be found . - pubmed
Lack of effect of domperidone on gastrin release : evidence for a peripheral activity of the drug . - pubmed
The lack of effect on gastrin release appears to confirm that domperidone is unable to penetrate the blood brain barrier in appreciable amounts in adults . - pubmed
In addition a portion of the enriched NK cells were positive for OKM1 , asialo - GM1 , and Ia antigens , which are undetectable on typical T cells but are present on PMN and/or monocytes . - pubmed
Zinc levels and , hence , SCL : SZL ratios did not reflect tumor activity . - pubmed
Vitronectin receptor expression and distribution at the photoreceptor - retinal pigment epithelial interface . - pubmed
A double - labeling procedure , employing VnR antibody and a fluorescently labeled phallotoxin ( phalloidin ) , was used to identify regions of VnR co - distribution with filamentous actin ( F - actin ) . - pubmed
Intravaginal clindamycin use caused a decrease 1 week post - therapy , but at 1 month , levels of lactobacilli were similar to those in the metronidazole treatment group . - pubmed
The intracellular localization of Cu / Zn - and Mn - superoxide dismutase ( SOD ) , which catalyze the dismutation of superoxide radicals ( O2- ) to O2 and H2O2 , was studied in the thyroid tissue of various thyroid disorders by an immunohistochemical technique . - pubmed
The concentrations of Cu / Zn - and Mn - SOD in thyroid tumor tissues and hyperplastic follicular disorders were significantly higher than those in normal thyroid tissue when they were compared as a function of protein or deoxyribonucleic acid contents . - pubmed
13C NMR relaxation data have been used to determine dipolar auto- and cross - correlation times for the di- and tripeptides GK , KG , and GKG , primarily to analyze lysine side - chain motional dynamics . - pubmed
Morphogenetic effect of kainate on adult hippocampal neurons associated with a prolonged expression of brain - derived neurotrophic factor . - pubmed
Therefore CA 19 - 9 and Sialyl Le ( x ) are tumor - associated antigens in the whole colorectum , and Leb and Le ( y ) in the distal colon . - pubmed
Expression of CA 19 - 9 in primary tumor provided more prognostic information and risk of liver metastasis than other Lewis blood group antigens . - pubmed
Exocytosis in neutrophils induced by thapsigargin and other inhibitors of calcium ATP - ase . - pubmed
Fluoride , a non - specific inhibitor of Ca ( 2 +)- ATPase , also causes exocytosis . - pubmed
Thapsigargin - induced exocytosis is not inhibited by L - type channel antagonists like verapamil , but is strongly inhibited by lanthanum ions , suggesting that the calcium required for induction of exocytosis is not entering via L - type channels , but via La ( 3 +)- sensitive calcium channels . - pubmed
The NGFr ( p75 )- IR identified with the electron microscope was located within small thinly myelinated and unmyelinated axons and within axon terminals . - pubmed
The profiles with NGFr ( p75 )- IR seen with the electron microscope indicate a primary afferent origin and show some similarities when compared to other markers of primary afferent fibers such as calcitonin gene - related peptide . - pubmed
In particular central catecholaminergic neurones and alpha - adrenoceptors have been analysed in detail , and they are recognized as important targets of the classic centrally acting antihypertensives clonidine , guanfacine and alpha - methyl - DOPA . - pubmed
Initially these drugs have been assumed to reduce elevated blood pressure via the stimulation of central alpha 2 - adrenoceptors in the brain stem , thus leading to peripheral sympathoinhibition and a reduction of elevated blood pressure , heart rate and plasma catecholamines . - pubmed
Finally , the peripheral alpha 1 - blocker urapidil has been demonstrated to possess an additional central mechanism , mediated by the stimulation of serotonergic 5HT1A - receptors located in the rostral ventrolateral medulla . - pubmed
The serotonin 5 - HT3 receptor is unique among the seven serotonin receptor " families " that have been recognized so far . - pubmed
Tyrosine phosphorylation was similar among MRL and normal CD4 + T cells after cross - linking with either anti - TCR antibody or anti - CD3 antibody , and following co - culture with Con A. - pubmed
In constrast , cross - linking of surface CD4 resulted in deficient tyrosine phosphorylation of cellular proteins in MRL T cells . - pubmed
The gL proteins of alpha herpesviruses and gamma herpesviruses do not have a significant percentage of amino acid sequence homology . - pubmed
Inhibition of dipeptidyl peptidase IV ( CD26 ) by peptide boronic acid dipeptides . - pubmed
Rate constants were determined for the inhibition of DPPIV using several peptide boronates at different pH values . - pubmed
Val - boroPro forms the most tightly bound complex with DPPIV ; the first order half life for dissociation of the inactive enzyme - inhibitor complex at 23 degrees C is approximately 27 days . - pubmed
VES , although having little effect on E2F-1 steady - state protein levels , decreased E2F-1 phosphorylation and transactivation activity and increased cyclin A binding to E2F-1 . - pubmed
The functional consequence of the negative regulatory effect of VES on E2F-1 function was shown by the ability of VES to inhibit the transcriptional activation of an E2F-1 responsive gene , c - myc . - pubmed
Anti - platelet action of nitric oxide and selective phosphodiesterase inhibitors . - pubmed
Since the NO effect is mediated via increased cGMP , this in vitro study was undertaken to test the hypothesis that selective phosphodiesterase ( PDE ) inhibitors might enhance aggregation inhibition at lower NO concentrations . - pubmed
Because the cAMP - selective PDE III and the cGMP - selective PDE V are prominent in platelets , milrinone , a PDE III inhibitor , and zaprinast , a PDE V inhibitor , were tested alone and in the presence of NO for their effect on aggregation . - pubmed
, formation of lipid peroxides , enlargement of HDL diameters , an increased exposure of the tryptophan groups of the apolipoprotein A - I to a more hydrophilic environment , formation of bityrosines , and cross - linking of apolipoprotein A - I. - pubmed
When these treatments were performed in the absence of EDTA , most of the modifications described above were more intense and HDLs formed a macroaggregate that displays a rosette - like structure . - pubmed
Native HDL or HDLs treated in the presence of EDTA inhibited the neuritogenesis of undifferentiated neurons but did not show any significant effect on the differentiated neurons in culture . - pubmed
Characterization of human IgG1 monoclonal antibody against gangliosides expressed on tumor cells . - pubmed
Despite a decrease in blood pressure , minoxidil had no effect on arterial weight or collagen content but increased elastin content in the abdominal aorta , renal , and superior mesenteric arteries . - pubmed
Thus the minoxidil - induced increase in arterial elastin content may be related to a direct effect of the drug to decrease elastase activity in these tissues . - pubmed
Although the nucleosome structure was formed as revealed by the micrococcal nuclease digestion , the B - type lamin uptake into the nuclei was inhibited by hemin . - pubmed
Some of the hemin - binding proteins bound concanavalin A ( Con A ) . - pubmed
Autoantibodies to steroid 21 - hydroxylase , 17alpha - hydroxylase , and P450 side chain cleavage enzyme were measured by immunoprecipitation assay using 35S - labeled recombinant proteins . - pubmed
Over recent years , a number of imidazole derivatives that specifically bind to the angiotensin II type 1 receptor , thereafter called sartans , have been developed and made available to the clinician . - pubmed
The results showed that Ca ( 2 +)- ATPase does not cause significant alterations in the interdigitation of 16:0 LPC / DPPC ( 27.0 mol% LPC ) vesicles when it is reconstituted with lipids . - pubmed
Intrinsic fluorescence , acrylodan fluorescent adducts , and CD spectra indicated that Ca ( 2 +)- ATPase , when embedded in interdigitated bilayer structures , is more exposed to the hydrophilic environment and has a looser structure than when embedded in non - interdigitated bilayers . - pubmed
Intracellular cholesterol biosynthesis may play a key role in supplying cholesterol ( as cholesteryl ester ) for the neutral core of very low density lipoprotein ( VLDL ) , thus modulating the secretion of apolipoprotein B-100 ( apo B-100 ) from hepatocytes . - pubmed
Pulse chase experiment demonstrated that NK-104 did not affect the synthetic rate of apo B-100 but increased intracellular degradation of newly synthesized protein . - pubmed
Experiments using cytochalasin A as an anti - cytoskeletal drug revealed that a functional actin cytoskeleton is crucial for mitochondrial morphology and the dynamic behavior of the mitochondrial network . - pubmed
Structure of the metal - water complex in Ras x GDP studied by high - field EPR spectroscopy and 31P NMR spectroscopy . - pubmed
On Mg ( 2 + ) --> ; Mn ( 2 + ) substituted samples , the first ligand sphere of the metal ion in the inactive , GDP - bound Ras has been studied by continuous wave EPR at 94 GHz ( W - band ) . - pubmed
However , for the alanine mutant in position 35 Ras(T35A ) as well as for the oncogenic mutant Ras(G12V ) , four water ligands can be observed in liquid solution . - pubmed
The EPR studies were supplemented by ( 31 ) P NMR studies on the Mg ( 2 + ) x GDP complexes of the wild - type protein and the three mutants . - pubmed
RESULTS : Cannabinoids were more effective antiemetics than prochlorperazine , metoclopramide , chlorpromazine , thiethylperazine , haloperidol , domperidone , or alizapride : relative risk 1.38 ( 95 % confidence interval 1.18 to 1.62 ) , number needed to treat 6 for complete control of nausea ; 1.28 ( 1.08 to 1.51 ) , NNT 8 for complete control of vomiting . - pubmed
Some potentially beneficial side effects occurred more often with cannabinoids : " high " 10.6 ( 6.86 to 16.5 ) , NNT 3 ; sedation or drowsiness 1.66 ( 1.46 to 1.89 ) , NNT 5 ; euphoria 12.5 ( 3.00 to 52.1 ) , NNT 7 . - pubmed
Harmful side effects also occurred more often with cannabinoids : dizziness 2.97 ( 2.31 to 3.83 ) , NNT 3 ; dysphoria or depression 8.06 ( 3.38 to 19.2 ) , NNT 8 ; hallucinations 6.10 ( 2.41 to 15.4 ) , NNT 17 ; paranoia 8.58 ( 6.38 to 11.5 ) , NNT 20 ; and arterial hypotension 2.23 ( 1.75 to 2.83 ) , NNT 7 . - pubmed
Second , Delta5 desaturase was more sensitive than Delta6 desaturase to changes in alcohol concentration . - pubmed
Third , in SHR , a tremendous decrease of arachidonic acid biosynthesis was evidenced in alcohol - intoxicated hepatocytes ; the effect was reinforced when ethanol concentration was high , mainly for Delta5 desaturase . - pubmed
METHODS : In situ hybridization with digoxigenin RNA probes localized cells responsible for gelatinases A and B , TIMP-1 and TIMP-2 mRNA production in histologic sections of aneurysmal ( n = 12 ) , and normal ( n = 6 ) cerebral arteries . - pubmed
Interferon - alpha-2b and ribavirin for retreatment of chronic hepatitis C. - pubmed
The physical interaction of HIF-2alpha with Ets-1 was demonstrated and localized to the HIF-2alpha carboxyl terminus and the autoinhibitory exon VII domain of Ets-1 , respectively . - pubmed
The deletion of the DNA binding and carboxyl - terminal transactivation domains of HIF-2alpha , respectively , created dominant negative mutants that suppressed transactivation by the wild type protein and failed to synergize with Ets-1 . - pubmed
Graft survival correlated with high dose cyclosporine treatment , complete complement blockade and the absence of an induced PAMA response to the murine anti - C5 monoclonal antibodies . - pubmed
An antagonist ( AM251 ) of the type-1 cannabinoid receptor blocked the depolarization - induced suppression of evoked inhibitory postsynaptic currents ( eIPSCs ) , and the cannabinoid WIN55,212 - 2 reduced eIPSC amplitude and occluded suppression . - pubmed
The degree of WIN55,212 - 2 - mediated inhibition of eIPSCs was strongly correlated with the magnitude of depolarization - induced suppression of the eIPSCs , suggesting that the WIN - sensitive afferents are suppressed by PN depolarization . - pubmed
The beta - site amyloid precursor protein cleaving enzyme 1 ( BACE1 ) , an integral membrane aspartyl protease responsible for cleavage of amyloid precursor protein ( APP ) at the beta - site , promotes A beta production . - pubmed
To analyze the protective effects of the aminotetraline derivative ( + /-)-( R , S )- 5,6 - dihydroxy-2 - methylamino-1,2,3,4 - tetrahydro - naphthalene hydrochloride ( CHF-1024 ) , a compound endowed with DA2 - dopaminergic / alpha2 - adrenergic receptor agonistic activity , in myocardial ischemia / reperfusion damage . - pubmed
This anti - ischemic effect of CHF-1024 was associated to a decrease in creatine kinase and lactate dehydrogenase , both released during heart reperfusion . - pubmed
These events were concomitant with maintenance of a higher production of 6 - keto - prostaglandin F1alpha The ability of CHF-1024 to improve postischemic ventricular dysfunction was correlated with a dose - dependent inhibition of the release of both norepinephrine ( NE ) , from sympathetic nerve endings , and TNF - alpha from cardiac tissue . - pubmed
The action of insulin on the penetration of sugars into the perfused heart . - pubmed
Olopatadine also significantly reduced ICAM-1 expression compared with placebo at 30 minutes and 5 hours postchallenge(P & lt ; 0.03 and P & lt ; 0.01 , respectively ) . - pubmed
Platelet binding of Stx was significantly higher in EDTA - washed preparations relative to ACD - derived platelets . - pubmed
Binding of Stx was also increased with ACD - derived platelets when activated with thrombin ( 1 U mL-1 ) and exposure of the Gb3 Stx receptor was detected only on platelets subjected to EDTA , higher g forces or thrombin . - pubmed
P - selectin ( CD62P ) exposure was significantly increased in EDTA - washed preparations relative to ACD - derived platelets , suggesting platelet activation . - pubmed
This study investigated apoptosis in prostate cancer before and after neoadjuvant treatment with LH - RH analog , demonstrating that this therapy induced high AI and bax and survivin overexpression , thus acting as a proapoptotic agent in prostate cancer . - pubmed
Here we test the hypothesis that glutaredoxin-1 ( Grx-1 ) , a member of the oxidoreductase family of enzymes , may be a critical component of redox - sensitive molecular switches by mediating reversible protein S - glutathionylation and enzymatic catalysis of thiol / disulfide exchange . - pubmed
ATP did not effect the chaperone activity of Hsc70 - SG but did increase the activity of reduced Hsc70 - SG . - pubmed
OBJECTIVE : Cardiac Troponin I ( cTnI ) phosphorylation by protein kinase C ( PKC ) results in a reduction of maximal actomyosin ATPase activity , an effect that is more marked at higher levels of calcium ( Ca2 + ) and is likely to reduce active force development . - pubmed
Thus , PKC phosphorylation sites on cTnI play a role in attenuating contractile responses to changes in intracellular Ca2 + . - pubmed
Moreover , plc1 appeared paler than the wild type , the result of an altered differentiation of chloroplasts and reduced chlorophyll levels compared with wild type filaments . - pubmed
In PJS patients , COX-2 was inhibited with a daily dose of 2 x 200 mg celecoxib for 6 months . - pubmed
We show that intra - LA infusions of the MAPK kinase ( MEK ) inhibitor U0126 , a manipulation which inhibits activation of ERK - MAPK , impairs postreactivation long - term memory ( PR - LTM ) but leaves the postreactivation short - term memory ( PR - STM ) intact . - pubmed
Post - reactivation infusions of cycloheximide blocked PR - LTM but not PR - STM and , in the absence of reactivation , had no effect . - pubmed
Resistance to thyroid hormone in a Chinese family with R429Q mutation in the thyroid hormone receptor beta gene . - pubmed
Diuretics , calcium antagonists , ACE inhibitors and angiotensin receptor blockers ( ARB ) are equally suitable for the reduction of blood pressure after apoplectic insult . - pubmed
The direct thrombin inhibitor , ximelagatran , will be available as an alternative to the oral anticoagulant marcumar in the foreseeable future . - pubmed
All forms of cyt c6 were functionally characterized by laser flash absorption spectroscopy , and their stability was probed by urea - induced folding equilibrium relaxation experiments and differential scanning calorimetry . - pubmed
Replacement of D72 by asparagine or lysine increased the efficiency of electron transfer to photosystem I , but destabilized the protein . - pubmed
pulses of methylprednisolone was observed , followed by a significant increase in BALP after completing the oral prednisone therapy . - pubmed
During steroid treatment of TO ( group I ) no dynamic changes of DPD levels were observed . - pubmed
Several features of MYPT2 and MYPT1 were similar , including : a specific interaction with the catalytic subunit of type 1 phosphatase , delta isoform ( PP1cdelta ) ; interaction of MYPT2 with the small heart - specific MP subunit ; interaction of the C - terminal region of MYPT2 with the active form of RhoA ; phosphorylation by Rho - kinase at an inhibitory site , Thr646 and thiophosphorylation at Thr646 inhibited activity of the MYPT2 - PP1cdelta complex . - pubmed
Overexpression of MYPT2 and PP1cdelta blocked the angiotensin II - induced sarcomere organization in cultured cardiomyocytes . - pubmed
Acetate is transported into the brain through MCA transporters that are also used for lactate and ketones . - pubmed
This upregulation would potentially allow a similar twofold increase in the transport of other MCAs , including lactate , during insulin - induced hypoglycemia . - pubmed
The enzyme can be inhibited by oxidant ( H ( 2 ) O ( 2 ) ) , solvent ( Tetrahydrofuran ) and several metal ions including Hg ( 2 + ) , Fe ( 2 + ) and Co ( 2 + ) . - pubmed
This ( R)-stereospecific epoxide hydrolase exhibits high enantioselectivity ( enantiomeric excess value , 99 % ) for the less hindered carbon atom of epoxide . - pubmed
Correspondingly , a reduced capacity for up regulation of CD62P expression and down regulation of CD42b was observed in PCT PLTs after stimulation by the agonists ADP or TRAP . - pubmed
Tsk ( 33.4+/-0.6 degrees C vs. 27.7+/-0.7 degrees C ) and Tre ( 37.4+/-0.6 degrees C vs. 37.0+/-0.6 degrees C ) were significantly higher in H. Tm was 38.7+/-1.1 degrees C in H. GE was lower in heat . - pubmed
Cocaine positive hairs and seized cocaine samples ( diluted to relevant concentrations ) were equally preprocessed and analyzed by LC - MS - MS . - pubmed
Thus , HIPK2 could induce beta - catenin protein degradation that was prevented by cell treatment with proteasome inhibitor MG132 . - pubmed
The FaCCD1 protein cleaves zeaxanthin , lutein , and beta - apo-8 '- carotenal in vitro . - pubmed
In the past decade , there are more than 2 000 published papers studying the clinical application , pharmacodynamic mechanism and structure - activity relationship ( SAR ) of berberine and its derivatives , for treating tumor , diabetes , cardiavascellum disease , hyperlipemia , inflammation , bacterium and virus infection , cerebral ischemia trauma , mental disease , Alzheimer disease , osteoporosis , and so on . - pubmed
This paper is a systematic review of the research advances of berberine and its derevatives in clinical application , pharmacodynamic mechanisms , molecular pharmacology , absorption and metabolism , and SAR studies . - pubmed
It has been reported that oral premedication with the H ( 2 ) receptor antagonist famotidine augmented intraoperative hypothermia . - pubmed
We again investigated whether the H ( 2 ) receptor antagonist famotidine significantly affected body temperature during open abdominal surgery under general anesthesia . - pubmed
In Digestive Disease Week 2008 , interesting advances in the treatment of hepatitis C were presented , especially related to the promising increase in the therapeutic efficacy of the combination of conventional treatment with the protease inhibitor , telaprevir . - pubmed
The cell cycle checkpoint status ( p21 , p27 , p16 , Tp53 ) , cell dynamics ( proliferating cell nuclear antigen , Ki-67 , terminal deoxynucleotidyl transferase - mediated dUTP - biotin nick - end labeling , M30 ) , DNA damage ( gamma - H2AX [ H2A histone family , member X ] ) , telomere lengths , chromosomal instability ( micronuclei index ) , and senescence - associated beta - galactosidase ( SA - beta - Gal ) activity were evaluated using an in situ approach and compared to those in normal liver ( n = 5 ) and liver with chronic cholestasis ( 34 cases of hepatolithiasis and three cases of primary biliary cirrhosis ) . - pubmed
Using 15 filter paper units of cellulase per gram cellulose , a digestibility of 95 % was achieved by the two - stage method , which was higher than 81 % obtained from a 1 % H ( 2 ) SO ( 4 ) pretreatment ( 200 degrees C , 8 min ) . - pubmed
The generation of reactive oxygen species ( ROS ) by the nicotinamide adenine dinucleotide phosphate oxidase is an important mechanism by which neutrophils kill pathogens . - pubmed
These components form an active complex at the correct time and subcellular location through a series of incompletely understood mutual interactions , regulated , in part , by GTP / GDP exchange on Rac , protein phosphorylation , and binding to lipid messengers . - pubmed
However , when the side chain of the midspan Arg ( 694 ) " snorkels " to the outer leaflet of the viral membrane , the MSD assumes a metastable conformation where the highly - conserved N - terminal core ( between Lys(681 ) and Arg(694 ) and containing the GXXXG motif ) unfolds . - pubmed
In contrast , when the Arg(694 ) side chain snorkels to the inner leaflet , the MSD peptide assumes a metastable conformation consistent with experimental observations where the peptide kinks at Phe(697 ) to facilitate Arg(694 ) snorkeling . - pubmed
RESULTS : SiP could reduce apoptosis rate ( P & lt ; 0.01 ) and stabilize the mitochondrial membrane potential ( P & lt ; 0.05 ) and improved the level of p - Akt1 ( P & lt ; 0.01 ) in hypoxia / reoxygenation cardiomyocytes significantly . - pubmed
Stabilization of mitochondrial membrane potential by P13K - AM signaling pathway is likely to play a role in protective action of S1P. - pubmed
Peroxidase activity was insensitive to oxidizing agents such as trans-4,4 - diydroxy-1,2 - dithiane or hydrogen peroxide , but was inhibited by treatment of thylakoids with reducing agents such as dithiothreitol or mercaptoethanol . - pubmed
We found that methylprednisolone not only significantly decreased serum TGF - beta1 , CTGF and troponin T , but also reduced myocardial expressions of TGF - beta1 , CTGF and Smad3 after CME . - pubmed
Stress treatments included sandblasting , leaf cutting and spraying with jasmonic acid , salicylic acid , chitosan oligosaccharide ( COS ) , H ( 2 ) O ( 2 ) ( HP ) and NaCl ( SC)at different concentrations . - pubmed
N - Terminal PEGylation of hGH was carried out by covalent attachment of PEG to the alpha - amine residue of Phe1 that yielded the monoconjugate PEG - Nter - hGH with a mass of 44152.2 Da , as measured by MALDI - TOF mass spectrometry . - pubmed
The mTGase mediated PEGylation , performed in a water / ethanol solution mixture , allowed a PEG coupling reaction only at the level of hGH Gln141 , yielding the single monoconjugate PEG - Gln141 - hGH with a mass of 44064.9 Da . - pubmed
Recognizing the potential pitfalls of hydrogen isotopic analysis of keratins with steam equilibration to infer origins of wildlife , food , and people . - pubmed
Analyses examined fractional anisotropy ( FA ) and mean diffusivity ( MD ) between adolescents with low and high levels of problematic alcohol use as assessed by the Alcohol Use Disorders Identification Test ( AUDIT ) . - pubmed
First , carboxylic acid - functionalized alkali lignin ( AL - COOH ) was prepared and subsequently incorporated into anhydride - cured epoxy networks via a one - pot method . - pubmed
MTT , colony formation assay and matrigel invasion assay were carried out to assess the role of cytoglobin on cell proliferation and invasion . - pubmed
After adjustment for potential confounders , when compared to trastuzumab patients , lapatinib patients had a higher rate of treatment discontinuation ( hazard ratio [ HR ] = 1.57 ; P & lt ; 0.001 ) , a higher rate of outpatient visits ( not treatment administration related ) ( IRR = 1.19 ; P & lt ; 0.004 ) , and a lower rate of medical visits associated with treatment administration ( IRR = 0.34 ; P & lt ; 0.001 ) . - pubmed
lactis CRL 581 possesses only one gene that encodes PrtL , which consists of 1924 amino acids and is a multidomain protein anchored to the cell via its W domain . - pubmed
PrtL was extracted from the cell under high ionic strength conditions using NaCl , suggesting an electrostatic interaction between the proteinase and the cell envelope . - pubmed
Under optimal conditions , PrtL exhibited higher affinity for succinyl - alanyl - alanyl - prolyl - phenylalanine - p - nitroanilide than for succinyl - alanyl - glutamyl - prolyl - phenylalanine - p - nitroanilide , while methoxy - succinyl - arginyl - prolyl - tyrosyl - p - nitroanilide was not degraded . - pubmed
Finally , on the basis of its specificity towards caseins and the unique combination of amino acids at residues thought to be involved in substrate specificity , PrtL can be classified as a representative of a new group of CEP . - pubmed
Anti - ERp5 antibody inhibited ERp5 - dependent platelet and endothelial cell disulfide reductase activity in vitro . - pubmed
ERp5 release at the thrombus site was detected after infusion of Alexa Fluor 488 - labeled anti - ERp5 antibody at 0.05 mug / g body weight , a dose that does not inhibit thrombus formation . - pubmed
The cysteine residues in the ERp5 active sites are not required for binding to beta3 integrin . - pubmed
Among therapy drugs that lower urate , benzbromarone ( BBR ) , an inhibitor of urate transporters , is widely used because it is well tolerated and highly effective . - pubmed
We demonstrate that BBR is also an activator of voltage - gated KCNQ potassium channels . - pubmed
In cultured recombinant cells , BBR exhibited significant potentiation effects on KCNQ channels comparable to previously reported classical activators . - pubmed
In native dorsal root ganglion neurons , BBR effectively overcame the suppression of KCNQ currents , and the resultant neuronal hyperexcitability caused by inflammatory mediators , such as bradykinin ( BK ) . - pubmed
Notably , the analgesic effects of BBR are largely mediated through peripheral and not through central KCNQ channels , an observation supported both by pharmacokinetic studies and in vivo experiments . - pubmed
Moreover , multiple residues in the superficial part of the voltage sensing domain of KCNQ channels were identified critical for the potentiation activity of BBR by a molecular determinant investigation . - pubmed
Our data indicate that activation of peripheral KCNQ channels mediates the pain relief effects of BBR , potentially providing a new strategy for the development of more effective therapies for gout . - pubmed
In group 3 , tail blood samples were collected from animals before ( T0 ) and 24 hours after ( T1 ) cyclophosphamide ( CP ) delivery . - pubmed
Hexavalent chromium - induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of alpha - tubulin . - pubmed
Cerdulatinib , a novel dual SYK / JAK kinase inhibitor , has broad anti - tumor activity in both ABC and GCB types of diffuse large B cell lymphoma . - pubmed
Herein , we investigated the anti - lymphoma activity of cerdulatinib , a novel compound that dually targets SYK and JAK / STAT pathways . - pubmed
Importantly , JAK / STAT and BCR signaling can be blocked by cerdulatinib in primary GCB and non - GCB DLBCL tumor cells that were accompanied by cell death . - pubmed
Our work provides mechanistic insights into the actions of cerdulatinib , suggesting that the drug has a broad anti - tumor activity in both ABC and GCB DLBCL , at least in part by inhibiting SYK and JAK pathways . - pubmed
Impact of CYP3A5 polymorphism on trough concentrations and outcomes of tacrolimus minimization during the early period after kidney transplantation . - pubmed
CONCLUSIONS : Although CYP3A5 polymorphism significantly influenced the tacrolimus dose required to achieve the target concentration , the impact of CYP3A5 polymorphism on BPAR was not observed in this study . - pubmed
Excess betaLGm preferentially entered the AMP phase at pH & gt ; 5.0 and the XG phase at lower pH. - pubmed
The inversion was caused by adsorption of XG onto betaLGm that started below pH 5.5 . - pubmed
Moreover , the increase of potassium currents by cisplatin was further inhibited by the application of TEA confirming the action of cisplatin on potassium channels . - pubmed
Moreover , the increase of potassium currents by cisplatin was further inhibited by the application of TEA confirming the action of cisplatin on potassium channels . - pubmed
RESULTS : Stretozotocin ( STZ ) only - treated groups exhibited hyperglycemia , polydipsia , polyuria , weight loss , glucose intolerance , low fasting plasma insulin and reduced hepatic glycogen content compared to the control group . - pubmed
Naringin treatment significantly improved Fasting Plasma Insulin ( FPI ) , hepatic glycogen content , malondialdehyde , beta - hydroxybutyrate , acetoacetate , bicarbonate , blood pH and anion gap but not Fasting Blood Glucose ( FBG ) compared to the STZ only - treated group . - pubmed
, ECG and respiratory motion gating ) ( 1 ) H - MRS was used to quantify myocardial triglyceride levels for each gender . - pubmed
CONCLUSIONS : The study showed that ( 1 ) H - MRS is a capable , noninvasive tool for acquisition of myocardial triglyceride metabolites . - pubmed
Investigation of binding properties of dicationic styrylimidazo [ 1,2 - a ] pyridinium dyes to human serum albumin by spectroscopic techniques . - pubmed
The binding interaction between two dicationic styrylimidazo [ 1,2 - a ] pyridinium dyes and human serum albumin ( HSA ) was investigated at physiological conditions using fluorescence , UV - vis absorption , and circular dichroism ( CD ) spectroscopies . - pubmed
The calculated thermodynamic parameters ( DeltaG , DeltaH and DeltaS ) indicated that hydrogen bonding and van der Waals forces played a major role in the formation of the dye - HSA complex . - pubmed
AIM : To comparatively evaluate the efficacy of magnesium sulphate as an adjunct to ropivacaine in local infiltration for postoperative pain following lower segment cesarean section . - pubmed
First tested these five concentrations of PCA2 ( 3 - 300 muM ) alone and in combination with BPA(100 mug / L ) , on insulin secretion from isolated islets at in vitro condition . - pubmed
At in vivo condition , the BPA ( 100 mug / kg ) and PCA2 ( 10 mumol / kg ) administered for 20 days then , blood glucose and insulin , Pdx1 and , Glut2 genes expression , and oxidative stress markers examined . - pubmed
The results indicated that PCA2 strongly prevents islet cells apoptosis induced by BPA and , co - administration of PCA2 and BPA modified hyperglycemia . - pubmed
BPA reduced Pdx1 and Glut2 mRNA expression and antioxidant level in pancreas tissue , whereas PCA2 prevented from these effects . - pubmed
The labelling efficiency with three stable specific immunoplasmonics labels ( functionalized silver nanospheres ( CD44 - AgNSs ) , gold ( Au ) NSs ( EGFR - AuNSs ) and Au nanorods ( KV1.1 - AuNRs ) ) detected by reflected light microscopy ( RLM ) is similar to the one with immunofluorescence . - pubmed
We genotyped two selected polymorphisms of the tryptophan hydroxylase 1 ( TPH1 ) gene ( rs1800532 218A>C and rs1799913 779A>C ) and polymorphism in the promoter region of serotonin transporter gene ( 5 - HTTLPR , rs25531 ) related to serotoninergic neurotransmission . - pubmed
This situation triggers a transcriptional response , mediated by the hypoxia inducible factors ( HIFs ) , that aims to restore oxygen homeostasis . - pubmed
RORalpha is a member of the nuclear receptor super family and plays important roles in lipid , glucose metabolism , as well as the inflammatory response . - pubmed
Conversely , selective antagonism ( SR1001 ) abrogated the induction of M2 macrophage markers which induced by CH - S. In addition , CH - S induced phosphorylation of Adenosine monophosphate ( AMP )- activated protein kinase alpha ( AMPKalpha ) , which was accompanied by the activation of acetyl - CoA carboxylase 1 ( ACC ) . - pubmed
However , SR1001 abolished the activation of AMPKalpha and ACC induced by CH - S. Meanwhile , inactivation of AMPKalpha by its inhibitor Compound C ( CompC ) abrogated the mRNA and protein levels of CH - S - induced M2 macrophage markers expressions . - pubmed
The non - receptor tyrosine phosphatase Ptpn11 ( Shp2 ) is an important transducer of growth factor and cytokine signals . - pubmed
PI3Ks have dual kinase specificity : a lipid kinase activity that phosphorylates the 3'-hydroxyl of phosphoinositides and a protein - kinase activity that includes autophosphorylation . - pubmed
Our results revealed that Lys-776 , located in the P - loop of PI3Kalpha , is essential for the recognition of lipid and ATP substrates and also plays an important role in PI3Kalpha autophosphorylation . - pubmed
Replacement of the residues His-936 and His-917 in the activation and catalytic loops , respectively , with alanine dramatically changed PI3Kalpha kinetics . - pubmed
Compared to the CM model group , the CM + AmB and CM + PD-1 Ab groups exhibited increased levels of Th1 cytokines and chemokines expression , and reduced levels of Th2 cytokines expressions . - pubmed
This study demonstrates that changes in circulating insulin that are too fine to adversely affect glucose homeostasis nonetheless exert control over adiposity.-Page , M. M. , Skovso , S. , Cen , H. , Chiu , A. P. , Dionne , D. A. , Hutchinson , D. F. , Lim , G. E. , Szabat , M. , Flibotte , S. , Sinha , S. , Nislow , C. , Rodrigues , B. , Johnson , J. D. Reducing insulin via conditional partial gene ablation in adults reverses diet - induced weight gain . - pubmed
Moreover , the presence of Snail or Twist1 partially blocked phorbol 12 - myristate 13 - acetate - induced megakaryocyte differentiation , while that of Twist significantly altered imatinib - induced erythroid differentiation . - pubmed
Lastly , coronal cells express the Trpa gene , which encodes an ion channel expressed in hair cells . - pubmed
Furthermore , BETi reduce growth of prostate cancer cells and patient - derived organoids with known AR mutations , AR amplification and AR - V7 expression . - pubmed
Conclusions : BETi merit clinical evaluation as inhibitors of AR splicing and function , with trials demonstrating their blockade in proof - of - mechanism pharmacodynamic studies . - pubmed
Hcp / fcc nucleation in bcc iron under different anisotropic compressions at high strain rate : Molecular dynamics study . - pubmed
In this study , a milk protein - derivative is shown to increase GHSR-1a - mediated intracellular calcium signalling in a concentration - dependent manner in vitro . - pubmed
Herein , we successfully synthesized partially oxidized 5 nm cobalt nanoparticles dispersed on a single - layer nitrogen - doped graphene ( SL - NG ) ( denoted as PO-5 nm Co / SL - NG ) catalyst by a unique and simple one - pot synthesis strategy , which was efficiently applied for highly selective electrocatalytic reduction of carbon dioxide to methanol in 0.1 mol dm-3 aqueous NaHCO3 medium under mild conditions , reaching the maximum faradaic efficiency ( FE ) of 71.4 % for methanol at -0.90 V versus saturated calomel electrode ( SCE ) , possessing a strong electrocatalytic current density of 4 mA cm-2 and a high yield of 1.10 mmol dm-3 h-1 , and the corresponding overpotential is as low as 280 mV. - pubmed
Moreover , at -1.0 V versus SCE , a high current density of 10 mA cm-2 can be obtained , and the FE for methanol still remains 23.2 % . - pubmed
This work first provides an advanced PO-5 nm Co / SL - NG for selective electroreduction of carbon dioxide into methanol , which simultaneously possesses the merits of high current density , low overpotential , high selectivity , superior FE , and good stability , outperforming most reported electrocatalysts . - pubmed
Determination of adsorbable organically bound fluorine ( AOF ) and adsorbable organically bound halogens as sum parameters in aqueous environmental samples using combustion ion chromatography ( CIC ) . - pubmed
Because of their toxicity and biomagnification potential individual perfluoroalkyl and polyfluoroalkyl substances ( PFAS ) are regularly examined in food and environmental matrices by LC - MS / MS . - pubmed
The combustion ion chromatography ( CIC ) can be used to determine adsorbable organic fluorine ( AOF ) in aqueous samples . - pubmed
CIC analysis was performed in 22 samples from a chemical company wastewater treatment plant , and 14 individual PFAS were determined by LC - MS / MS . - pubmed
AOF values up to 555 mug / L were found in these samples while the total of the individual PFAS , calculated as fluorine , was 8.8 mug / L. This provides evidence , that CIC covers a huge range of fluoroorganic compounds that are presently not detected by LC - MS / MS . - pubmed
Furthermore , the CIC method allowed the determination of four halogens in 26 surface water samples . - pubmed
This demonstrated that the CIC technique can be used as a powerful screening test to support LC - MS / MS methods , and is also useful to detect organic chlorine , bromine and iodine compounds ( AOCl , AOBr and AOI ) . - pubmed
The aim of this study was to investigate the distribution and retention of different chemotypes typifying available inhaled drugs [ slowly dissolving neutral fluticasone propionate ( FP ) and soluble bases salmeterol and salbutamol ] using mass spectrometry imaging ( MSI ) . - pubmed
In the same animals , salmeterol - d3 , salbutamol - d3 , and FP - d3 were delivered by intravenous ( IV ) injection . - pubmed
At the 30 - minute time point , inhaled salmeterol and salbutamol were preferentially retained in bronchiolar tissue , whereas FP was retained in all regions of the lungs . - pubmed
5 ' RACE experimental data showed that bna - miR827a cleaves three NITROGEN LIMITATION ADAPTATION ( NLA ) genes ( BnaC08g45940D , BnaA10g01450D and BnaC05g01480D ) . - pubmed
The expression levels of a phosphate ( Pi)-related miRNA , bna - miR827a , and its target genes were affected in wax - deficient rapeseeds . - pubmed
Among all of the nitroaromatics , the highest fluorescence quenching is observed for nitrophenols ( say picric acid ( PA ) ) . - pubmed
Perrin 's model of quenching sphere action has been attributed to nitrophenol ( PA ) selective signaling behavior of biaryl systems . - pubmed
Synergistic interactions were observed when DosRST inhibitors were examined in pairwise combinations with the strongest potentiation observed between artemisinin paired with HC102A , HC103A , or HC106A. - pubmed
GRP94 is an ATP - dependent chaperone able to regulate pro - oncogenic signaling pathways . - pubmed
In this work , an untargeted lipidomic analysis revealed that some lipid species were altered in GRP94 - deficient cells , specially GM2 and GM3 gangliosides . - pubmed
The catalytic pathway of GM2 is affected by the low enzymatic activity of beta - Hexosaminidase ( HexA ) , responsible for the hydrolysis of GM2 to GM3 . - pubmed
In this work , based on the strategy of label - assisted chemical adsorption triggered conversion of electroactivity of sensing interface , a Ag / AgCl process was achieved to enhance sensitivity of the constructed sandwich - type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 19 - 9 ( CA19 - 9 ) . - pubmed
The ' Coal Replacing Project ' ( CRP ) , replacing coal with cleaner energy like natural gas and electricity , was implemented in North China to curb PM2.5 pollution ; therefore , it is important to explore the sources and transformation mechanisms of PM2.5 nitrate under this strategy for examining its effectiveness . - pubmed
The results showed that the average concentrations of PM2.5 and its dominant inorganic ions ( SO42 - , NO3 - , NH4 + ) in C3 during the CRP , compared to C2 , decreased by 62.13 % , 79.69 % , 55.14 % and 38.84 % , respectively , attesting the improvement of air quality during the CRP . - pubmed
According to the correlation between [ NO3 -/ SO42 - ] and [ NH4 +/ SO42 - ] as well as delta18O variations , the homogeneous formation pathway might be dominant in C1 , while the heterogeneous pathway would be primary in C2 and C3 during the formation of nitrate . - pubmed
The dominant sources in C1 were biogenic soil emission ( 37.0 % + - 9.9 % ) and mobile emission ( 25.7 % + - 19.1 % ) , while coal combustion ( 42.4 % + - 13.8 % in C2 and 34.9 % + - 14.4 % in C3 ) and biomass burning ( 31.0 % + - 21.2 % and 34.7 % + - 22.7 % ) were the main sources in C2 and C3 . - pubmed
In the winter , the contribution of coal combustion dropped by about 8 % during the CRP ( C3 ) in comparison with that in C2 , suggesting the implementation of CRP played an important role in reducing NOX from coal combustion . - pubmed
Further studies revealed that MS prevented CSDS - evoked augmentation of glutamatergic transmission onto principal neurons ( PNs ) in the basolateral amygdala ( BLA ) by inhibiting presynaptic glutamate release . - pubmed
Diclofenac sodium - loaded sodium alginate / hydroxyapatite / halloysite nanotubes ( SA / HA / HNTs - DS ) nanocomposite hydrogel beads were prepared by the in situ generation of HA nanoparticles during the sol - gel transition of the SA / HNTs suspension . - pubmed
, the weight ratio of HNTs to SA ( mHNTs / mSA ) , the weight ratio of ( NH4 ) 2HPO4 to SA ( m ( NH4 ) 2HPO4 /mSA ) , and the concentration of SA , which influenced the entrapment efficiency ( EE ) and release of DS , were investigated . - pubmed
An almost constant rate release of DS is achieved when mHNTs / mSA = 0.3 and m( NH4 ) 2HPO4 /mSA = 0.1 . - pubmed
Simeprevir , daclatasvir , and sofosbuvir for hepatitis C virus - infected patients : Long - term follow - up results from the open - label , Phase II IMPACT study . - pubmed
BACKGROUND : Hyaluronic acid ( HA ) is a key contributor to skin moisture ( hydration ) , and Microbotox demonstrates improvements for fine wrinkles of the face . - pubmed
Using the hydrotoxin mixture , two ingredients could be injected on the exact dermal layer of faces in a session . - pubmed
This inhibitory effect of VEGF was not influenced by masking of phosphatidylserine ( PS ) on the surface of apoptotic cells and was partially mediated by the PI3K - Akt signaling pathway . - pubmed
After the microcosms were sparged with hydrogen gas , samples were taken regularly within 122 h for nitrate and nitrite measurements , community composition analysis via 16S rRNA gene amplicon sequencing , and gene and transcript quantification via qPCR of reductase genes essential for complete denitrification . - pubmed
In this study , a multicolor sensing system has been established for simultaneous determination of four foodborne bacteria by exploiting oxidase mimicking activity of aptamer - functionalized manganese dioxide - coated ferriferrous oxide ( apt - Fe3O4 / MnO2 ) nanocomposites and oxTMB etching of gold nanorods ( AuNRs ) . - pubmed
Effect of various inhibitors of factor XIII on the patterns in SDS - electrophoresis and crossed immunoelectrophoresis . - pubmed
The effect of different factor XIII inhibitors ( competetive inhibition , interference with active center SH - groups in different ways , Ca2 + depletion ) on the sequence of the gamma - dimerization and alpha - polymerization of fibrin is examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis ( SDS - PAE ) ( reduced material ) . - pubmed
Non - crosslinked fibrin and fibrin clots of different degrees of crosslinking are digested with plasmin and the lysate tested in SDS - PAE ( non - reduced material ) and crossed agarose gel immunoelectrophoresis ( CAIE ) . - pubmed
Minimally destructive methods were sought to assess the factor VIII content of fresh frozen plasma intended for large - scale fractionation and stored in five - liter polyethylene packs after pooling approximately 23 plasma donations . - pubmed
While not directly inhibiting the growth of subcutaneous fibroblasts in vitro , dexamethasone does suppress the production and/or activity of fibroblast growth factors generated through macrophage - EL4 cell interactions in vitro . - pubmed
We have examined the effects of fetal hypoxaemia , produced by reducing the percent oxygen in maternal inspired air , on fetal plasma concentrations of corticotrophin releasing hormone ( CRH ) , adrenocorticotrophin ( ACTH ) and cortisol and determined the effects of an opioid receptor antagonist , naloxone on these responses . - pubmed
Hypoxaemia ( fetal PO2 , 15 - 18 mmHg ) for 60 min provoked a significant ( P & lt ; 0.05 ) increase in fetal plasma ACTH and cortisol concentrations at days 125 - 130 of pregnancy , but did not affect circulating CRH . - pubmed
There was no effect of naloxone administered either intravenously ( 1.25 mg bolus followed by a 2.5 mg / h continuous infusion for one hour ; fetal body weight approximately 2.5 Kg ) or via the lateral cerebral ventricle ( 50 micrograms bolus followed by a 100 micrograms / h infusion for one hour ) on this pattern of ACTH and cortisol change nor on the lack of CRH response to hypoxaemia . - pubmed
We conclude that the increase in fetal ACTH and cortisol in response to acute hypoxaemia is not accompanied by an increase in systemic CRH concentrations , nor is the response dependent on short - term opioid regulation . - pubmed
Induction of this enzyme was accompanied by a decrease in the constitutive NO synthase activity , a phenomenon which was prevented or reversed by dexamethasone ( 1 microM ) . - pubmed
Cytochalasans and PMA induce IL-2 receptors on CD8 + lymphocytes . - pubmed
The phorbol ester tumor promoter , PMA , alone is not mitogenic for primary lymphocytes but enhances the activity of mitogenic lectins . - pubmed
Because the cytochalasans have been reported to increase intracellular Ca2 + and because PMA activates protein kinase C , lymphocytes were treated with PMA and cytochalasin B ( CyB ) to determine if this combination would induce DNA synthesis . - pubmed
While this treatment by itself did not cause proliferation , lymphocytes cultured with PMA and CyB overnight , washed , and recultured with IL-2 proliferated to the same degree as lymphocytes stimulated with Con A. - pubmed
Three different cytochalasans , cytochalasin B , cytochalasin D , and chaetoglobosin C , all of which bind to cellular actin with different affinities and only one of which affects glucose transport , induced IL-2 receptors in combination with PMA . - pubmed
However , no IL-2 was produced after the exposure of lymphocytes to the combination of cytochalasans and PMA . - pubmed
We recently reported that autophosphorylated protein kinase C ( PKC ) has an intrinsic Ca ( 2 +)- and phospholipid - dependent ATPase activity and that the ATPase and histone kinase activities of PKC have similar metal - ion cofactor requirements and Km , app(ATP ) values . - pubmed
These observations support a mechanism of PKC catalysis in which the productive binding of phosphoacceptor substrates enhances the rate of phosphodonor substrate ( ATP ) hydrolysis at the active site of PKC . - pubmed
In fact , sequence analysis indicates that PKC contains a potential second nucleotide binding site outside of its active site . - pubmed
We show that the regulatory and catalytic properties of the ATPase reactions of three PKC isozymes are similar , despite critical differences among the isozymes in their consensus sequences for the potential non - active - site nucleotide binding site in their catalytic domains . - pubmed
We also show that the ATPase and histone kinase reactions of each isozyme have similar Km , app(ATP ) values. - pubmed
Two and five days after FAS injections into the amygdala , the density of muscarinic receptor decreased about 50 % as measured by the specific bindings of 3H - quinuclidinyl benzilate and 3H - oxotremorine . - pubmed
The production of inositol bisphosphate and the release of Ca2 + from intracellular stores induced by IgE - antigen stimulation were enhanced in 3C7 . - pubmed
Hypolipidemic effect of polymethylenemethane thiosulfonates : inhibitors of acetoacetyl coenzyme A thiolase . - pubmed
Their action was based on their specific inhibitory effect on cytoplasmic acetoacetyl - CoA thiolase , one of the key enzymes in cholesterol biosynthesis . - pubmed
In the presence of Mg2 + alone , the leuX precursor was found to be self - cleaved at a site approximately 13 nt inside from the 5 ' end of mature tRNA . - pubmed
The basic comparison was made from the staining patterns for the myofibrillar actomyosin ATPase produced by preincubation of fresh frozen cross - sections of muscle at alkaline pH ( 10.30 ) or acid pH ( 4.60 ) with those produced by preincubation in media containing Cu2 + at alkaline pH ( 10.30 ) , near neutral pH ( 7.40 ) , or acid pH ( 4.60 ) . - pubmed
Type II fibers in mixed fibered muscles were either very sensitive , moderately sensitive , or relatively insensitive to inactivation of the myofibrillar actomyosin ATPase after acid preincubation . - pubmed
The single prostacyclin receptor of gel - filtered platelets provides a correlation with antiaggregatory potency of PGI2 mimics . - pubmed
Platelet - rich plasma ( PRP ) displays the same receptor density but the KD value increases to 123 nM due to protein binding of PGI2 which lowers its effective concentration . - pubmed
Antiaggregatory IC50s and radioligand binding IC50s correlate for PGE2 , E1 , and six PGI2 analogs . - pubmed
In group I , atropine increased sinus rate from 53.7 + /- 4 to 87.9 + /- 17 bpm ( delta % : 65.5 + /- 33 ) and in group II from 51.6 + /- 5 to 73.9 + /- 14 bpm ( delta % : 43.1 + /- 26 ) . - pubmed
The adherence was decreased by platelets and the active constituent was identified as adenosine diphosphate which decreased the sticking of fibrin to cellular linings as well as to artificial surfaces . - pubmed
Ribonucleotide reductase is responsible for the production of the deoxyribonucleotides required for DNA synthesis . - pubmed
Bt2cAMP ) results in the protein kinase - dependent arrest of the cells in the G1 phase of the cell cycle . - pubmed
Removal of bt2cAMP after 24 h exposure resulted in increased holoenzyme and protein M2 activities . - pubmed
These results demonstrate that the ribonucleotide reduction necessary for DNA synthesis is regulated in a cell cycle - dependent fashion by the activity of the protein M2 subunit of ribonucleotide reductase . - pubmed
A PifC - LacZ fusion protein of 149,000 daltons has been identified by immunoprecipitation and polyacrylamide gel electrophoresis . - pubmed
The maximum hemagglutination titer ( 1:64 to 1:256 ) was obtained against GA - fixed RBC with the KSCN - treated organisms that were also sonicated . - pubmed
The activity was inactivated by heating at 100 C or by treatments with formalin or trypsin , but not by treatments with hyaluronidase or neuraminidase . - pubmed
Evidence for a carboxyl group in the vicinity of the retinal chromophore of bacteriorhodopsin . - pubmed
Carboxyl groups of bacteriorhodopsin in purple membranes were activated using a hydrophobic reagent and then covalently labeled with a pH - sensitive reporter group , nitrotyrosine methyl ester . - pubmed
These results indicate that a carboxyl group is near the retinal chromophore of bacteriorhodopsin . - pubmed
Mutually facilitated binding of cholesterol and adrenodoxin . - pubmed
The addition of PCC also resulted in the modest expression of B7 - 1 and a more robust expression of B7 - 2 on the cognate B cells . - pubmed
The S6 monoclonal antibody to somatostatin , but not control antibodies , enhanced gastrin - stimulated histamine release . - pubmed
In the presence of S6 , the somatostatin analogue SMS-201 - 995 ( 10(-7 ) M ) inhibited gastrin - stimulated histamine release by 95 % . - pubmed
In contrast to somatostatin , epinephrine failed to inhibit this gastrin stimulation . - pubmed
Furthermore , enhancement of gastrin - stimulated histamine release by immunoneutralization of somatostatin indicates an important role for endogenous somatostatin as a paracrine inhibitor of non - mast cell histamine release . - pubmed
MAIN OUTCOME MEASURES : We measured blood pressure , serum creatinine , hematocrit , and hemoglobin . - pubmed
RESULTS : Bone lead levels in the patella were found to be significantly correlated with a decrease in hemoglobin and hematocrit , even after adjusting for age , blood lead , body mass index , cigarette smoking , and alcohol ingestion and removing outliers . - pubmed
Several other agents including pyrazole , 4 - methylpyrazole , isoniazid , pyridine , and DMSO also increased the content of P4502E1 in this cell line but not oxidation of substrates , presumably a reflection of remaining tightly bound to the active site of P4502E1 . - pubmed
Slot blot analysis indicated that ethanol addition did not increase P4502E1 mRNA levels . - pubmed
These results indicate that ethanol can increase the content of P4502E1 as well as catalytic oxidation of substrates dependent on P4502E1 in this experimental model , perhaps by stabilization of the protein against degradation . - pubmed
The different samples were homogenized and centrifuged ( 2000 g during 20 minutes ) , and the supernatant was incubated with dog kininogen and 0.1 mol / L Tris - HCl buffer ( pH 8.5 ) in the presence of peptidase inhibitors . - pubmed
Tin - protoporphyrin ( 10 mumol / L ) produced an eightfold to 10 - fold increase in erythropoietin RNA within 40 min . - pubmed
Exposure to cobalt chloride ( 100 mumol / L ) resulted in a 20 - fold to 30 - fold increase in erythropoietin expression for 5 to 20 min , returning to control by 40 min . - pubmed
Additionally , nuclear runoff assays demonstrated that the increase in heme oxygenase or erythropoietin messenger RNA accumulation by cobalt chloride appeared to be a result of stimulated transcription of the heme oxygenase and erythropoietin genes . - pubmed
Heme produced an immediate expression of heme oxygenase RNA ( 50 - fold within 5 min ) and a second sustained response during the next 24 hr . - pubmed
Thus both erythropoietin and heme oxygenase genes appear to be expressed after treatment with tin - protoporphyrin , heme or cobalt chloride ; however , the time and patterns of expression are different. - pubmed
In this report we present the construction and analysis of 15 mutant alleles of the topoisomerase containing amino acid substitutions in a highly conserved region . - pubmed
In sum , these studies suggest that this region of the topoisomerase ( amino acids 216 - 225 ) plays a proximal role in mediating DNA cleavage and the covalent interaction between the 3'-phosphoryl of the nicked DNA and tyrosine 274 of the vaccinia topoisomerase I. - pubmed
The number of macrophages and their response to LH to produce IL-1 were increased significantly ( P & lt ; 0.01 ) by estradiol treatment but not by progesterone or testosterone treatment . - pubmed
Our results show that the higher concentrations of ethylenediaminetetraacetic acid ( EDTA ) and bovine serum albumin ( BSA ) in CZB medium , in comparison with KSOM , protect against the toxic component in the oil . - pubmed
Unlike coiled coil motor proteins , mitofilin is resistant to detergent extraction . - pubmed
The presence of mitochondrial targeting and stop - transfer sequences , along with the accessibility of mitofilin to limited proteolysis suggests that it resides predominantly in the intermembrane space , consistent with immuno - electron micrographs which show mitofilin mainly at the mitochondrial periphery . - pubmed
The induced increase in cytosolic [ Ca2 +]i is blocked by sugar hapten inhibitors of galectin-3 . - pubmed
The galectin-3 - induced effect is insensitive to voltage - gated Ca2 + channel antagonists such as prenylamine , nifedipine and diltiazem and to pertussis toxin but is inhibited by cholera toxin . - pubmed
The results suggest that galectin-3 released by accessory cells such as macrophages may bind in vivo to T - cell activation antigens and also participate in Ca2 + signalling . - pubmed
The DNA helicases acting in nucleotide excision repair , XPD , CSB and XPB , are not required for PCNA - dependent repair of abasic sites . - pubmed
DNA repair of abasic sites is accomplished in mammalian cells by two distinct base excision repair ( BER ) pathways : a single nucleotide insertion pathway and a proliferating cell nuclear antigen ( PCNA)-dependent pathway involving a resynthesis patch of 2 - 10 nucleotides 3 ' to the lesion . - pubmed
The latter pathway shares some enzymatic components with the nucleotide excision repair ( NER ) pathway acting on damage induced by ultraviolet light : both pathways are strictly dependent on PCNA and several observations suggest that the polymerization and ligation phases may be carried out by common enzymatic activities ( DNA polymerase delta / epsilon and DNA ligase I ) . - pubmed
No defect of either the PCNA - dependent or the single nucleotide insertion pathways could be observed in UV5 , UV61 or 27 - 1 mutant cell extracts , thus showing that the partial enzymatic overlap between PCNA - dependent BER and NER does not extend to DNA helicase activities . - pubmed
Expression of heme oxygenase-1 mediated by non - NMDA and metabotropic receptors in glial cells : possible involvement of reactive oxygen species production and protein kinase C activation . - pubmed
Heme oxygenase ( HO ) produces biliverdin and bilirubin which are physiological antioxidants and potent scavengers of oxygen radicals . - pubmed
The HO-1 protein level was significantly enhanced by several agonists for non - N - methyl - D - aspartate ( non - NMDA ) receptors and metabotropic glutamate receptors ( mGluR ) such as KA , quisqualic acid ( QA ) , ( + /-)- alpha - amino-3 - hydroxy-5 - methylisoxazole-4 - propanoic acid ( AMPA ) , and trans -(+/-)- 1 - amino -( 1S,3R )- cyclopentanedicarboxylic acid ( ACPD ) . - pubmed
KA - induced HO-1 expression was inhibited by the non - NMDA antagonist NBQX . - pubmed
In addition , KA induced the marked production of reactive oxygen species ( ROS ) , and KA - induced HO-1 expression was also inhibited by the antioxidants allopurinol and ascorbic acid . - pubmed
ACPD - induced HO-1 expression was inhibited by the mGluR antagonist MCPG and the protein kinase C ( PKC ) inhibitor calphostin C. - pubmed
Vesicular monoamine transporters ( VMATs ) are involved in chemical transduction in monoaminergic neurons and various endocrine cells through the storage of monoamines in secretory vesicles . - pubmed
The 5 - HT uptake was inhibited significantly by bafilomycin A1 ( an inhibitor of vacuolar H +- ATPase ) , 3,5 - di - tert - butyl-4 - hydroxybenzylidenemalononitrile ( a proton conductor ) , or reserpine ( an inhibitor of VMAT ) . - pubmed
The 5 - HT immunoreactivity in the long branching processes disappeared upon incubation with reserpine . - pubmed
Our results suggest that PTH modulates the expression of ClC-5 in the kidney cortex and that neither 1alpha,25 ( OH )( 2 ) vitamin D ( 3 ) nor PTH regulates ClC-5 expression in the medulla . - pubmed
The pattern of expression of ClC-5 varies with urinary calcium . - pubmed
Insulin increases renal sodium reabsorption which may contribute to hypertension . - pubmed
The meglitinides are nonsulfonylurea agents that are also insulin secretagogues . - pubmed
Unlike the sulfonylureas , repaglinide appears to require the presence of glucose to close the adenosine triphosphate - sensitive potassium channels and induce calcium influx . - pubmed
Disaccharidase inhibitors effectively compensate for the defective early - phase insulin release by slowing the production of sugars from carbohydrates . - pubmed
Thiazolidinediones appear to activate peroxisome proliferator - activated receptor gamma , which is involved in the metabolism of lipids . - pubmed
The combined use of the early detection of alcohol consumption ( EDAC ) test and carbohydrate - deficient transferrin to identify heavy drinking behaviour in males . - pubmed
The aim of this study was to determine the efficacy of the combined use of carbohydrate - deficient transferrin ( CDT ) and the Early Detection of Alcohol Consumption ( EDAC ) test to assess heavy drinking in a population of males ( n = 187 ) drinking an average of 20 drinks per day . - pubmed
Siglecs are immunoglobulin superfamily member lectins that selectively recognize different types and linkages of sialic acids , which are major components of cell surface and secreted glycoconjugates . - pubmed
Our results are consistent with a model whereby phosphatidic acid generated from phosphatidylcholine hydrolysis by Spo14p regulates susceptibility to choline - containing lysolipid analogs and drugs . - pubmed
A 2 - oxoglutarate - dependent dioxygenase is integrated in DIMBOA - biosynthesis . - pubmed
Comparing C12 with sodium caprate ( C10 ) , used in drug products marketed , 100 micromol C10 was needed to exert the similar absorption - enhancement of rebamipide , a poorly absorbable antiulcer drug , to that by 10 micromol C12 , and 100 micromol C10 was obviously more toxic to the mucosa than 10 micromol C12 . - pubmed
Univariate and multivariate analyses predicted systolic and diastolic sodium sensitivity based on interactions among demographic factors ( age , gender , race , body mass index ) and hypertensive type [ low - renin ( LR ) , modulator ( M ) , non - modulator ( NM ) ] . - pubmed
CONCLUSIONS : For subjects in sodium balance , the distributions of SSS and DSS were unimodal and the mechanisms mediating SSS and DSS were different . - pubmed
OBJECTIVES : DA-8159 is a pyrazolopyrimidinone derivative showing potent and selective phosphodiesterase 5 ( PDE5 ) inhibition . - pubmed
Purification of scotopsin and its amino acid component . - pubmed
Myocardial carbohydrate metabolism during induced cardiac arrest and post - arrest perfusion . - pubmed
[ Assessment of several years of the treatment with ACTH and corticosteroids of chronic total pelada ] . - pubmed
Circulatory response to tilt with hydroxyzine , thiethylperazine maleate , SA 97 . - pubmed
The toxicity of tumor necrosis factor - alpha upon cholinergic neurons within the nucleus basalis and the role of norepinephrine in the regulation of inflammation : implications for Alzheimer ' s disease . - pubmed
The loss of forebrain norepinephrine following systemic treatment with DSP4 did not alter the level of cortical choline acetyltransferase activity or activate microglia but significantly activated astrocytes within the basal forebrain . - pubmed
By using artificial polyleucine TM segments we have demonstrated in this study that a pattern of only five amino acids ( GVxxGVxxT ) promotes specific dimerisation . - pubmed
Single nucleotide polymorphisms of the IL18 gene are associated with atopic eczema . - pubmed
In sharp contrast with styrylquinolines bearing a carboxylic acid group at C-7 , these compounds proved to be inactive toward HIV-1 integrase in in vitro assays . - pubmed
Production of indoleamine 2,3 - dioxygenase ( IDO ) by tumor cells , leading to tryptophan depletion and production of immunosuppressive metabolites , may facilitate immune tolerance of cancer . - pubmed
However , 3 - HAA , but not L - kyn markedly inhibited antigen - independent proliferation of CD8(+ ) T cells induced by common receptor gamma - chain cytokines IL-2 , - 7 and - 15 . - pubmed
Our data suggest that CD8(+ ) T cell activation induced by antigenic stimulation , a function exquisitely fulfilled by mDC , is unaffected by tryptophan metabolites . - pubmed
Instead , in the absence of effective T cell receptor triggering , 3 - HAA profoundly affects homeostatic proliferation of CD8(+ ) T cells . - pubmed
Ferritin , the iron storage protein , plays a key role in iron metabolism . - pubmed
The predicted protein contains 170 amino acid residues with a predicted molecular weight ( MW ) about 19 , 422.89 Da and theoretical isoelectric point ( PI ) of 4.73 . - pubmed
A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro . - pubmed
These results suggest that protein phosphatase 1 or 2A , or both , are involved in the regulation of Ca ( 2 + ) uptake across the plasma membrane , in exocytotic activity and in the biosynthesis of cell wall components , all processes that occur in the tip region of pollen tubes and that control pollen tube development . - pubmed
Racecadotril , which is rapidly metabolized to its active metabolite thiorphan , is a neutral endopeptidase ( NEP ) inhibitor used in clinical practice for diarrhoea with a remarkable safety profile . - pubmed
Intraperitoneal administration of thiorphan reduced ibotenate - induced cortical lesions by up to 57 % and cortical caspase-3 cleavage by up to 59 % . - pubmed
Thiorphan effects were mimicked by substance P ( SP ) and , in a less potent manner , by neurokinin A. - pubmed
Thiorphan effects were inhibited by blockers of NK1 and NK2 receptors . - pubmed
In particular , potential bioenergetic and neural mechanisms for anticonvulsant efficacy of fluoxetine were explored using food intake / body weight monitoring and quantification of brain serotonin transporter protein . - pubmed
Importantly , the 7 day period of dietary fluoxetine exposure was effective in selectively reducing cell density in the parietal cortex and increasing serotonin transporter protein content in the nucleus accumbens . - pubmed
Recent genetic evidence has linked mutations in hTHTR1 with the manifestation of TRMA ( thiamine - responsive megaloblastic anaemia ) , a condition also associated with diabetes mellitus , sensorineural deafness and retinal disorders . - pubmed
Our results reveal a spectrum of mutant phenotypes , underlining that TRMA can result from decreased thiamine transport activity underpinned by changes in hTHTR1 expression levels , cellular targeting and/or protein transport activity . - pubmed
The results indicate that manic or mixed recurrences might rather occur at lower lithium levels , whereas the depressive pole prevails in the higher range . - pubmed
The reduction of prochiral ketones catalyzed by Ru ( diphosphine )( diamine ) complexes has been studied at the DFT - PBE level of theory . - pubmed
The DFT - PBE results suggest that this reaction is driven to the ( R)-product only by the process of binding the acetophenone to the active site of the trans - Ru ( H ) 2 ( S , S - dpen )( S - tolbinap ) catalyst . - pubmed
In fact , autoantigens have been demonstrated to be content within apoptotic bodies and apoptotic cells seems to be critical in the presentation of antigens , activation of innate immunity and regulation of macrophage cytokine secretion . - pubmed
The atheroprotective effect of apo B peptide immunization occurred in the absence of an increase in peptide - specific IgG , but was associated with an increase in IgM recognizing native and copper - oxidized LDL . - pubmed
Analysis of xenon binding to photosystem II by X - ray crystallography . - pubmed
Under the conditions employed , 25 Xe - binding sites were identified in each monomer of the dimeric PSII complex . - pubmed
One binding site was located close to the haem of cytochrome b559 in a position analogous to a Xe - binding site of myoglobin . - pubmed
The only Xe - binding site not associated with the intrinsic subunits of PSII was within the hydrophobic core of the PsbO protein . - pubmed
These altered behavioral responses to LPS were accompanied by reduction in dopamine turnover within the striatum and increased expression of cyclooxygenase-2 in the cortex . - pubmed
It has been shown that diabetes - induced fetal abnormalities are accompanied by some metabolic disturbances including elevated superoxide dismutase ( SOD ) activity , reduced levels of myoinositol and arachidonic acid and inhibition of the pentose phosphate shunt pathway . - pubmed
The activation of PGU1 transcription by galactose was found to be strain specific , independent of the strain being an industrial or a domesticated one . - pubmed
OBJECTIVE : To detect the release of endostatin in microcapsules of calcium alginate gel by emulsification - internal gelatification technology , to observe the distribution of Endostatin microcapsules in the eye by periocular injection and biocompatibility . - pubmed
METHODS : The calcium alginate gel microcapsules encapsulating endostatin were prepared by emulsification - internal gelatification technology , the release of endostatin in microcapsules in vitro was examined by uv - Spectrophotometry . - pubmed
RESULTS : SDS polyacrylamide gel electrophoresis showed strap of ES protein . - pubmed
One chemotherapy cycle consisted of irinotecan 180 mg / m(2 ) on days 1 and 15 ; 5 - FU 400 mg / m(2 ) bolus IV with 600 mg / m(2 ) by a 22 hour intravenous infusion on days 1 , 2 , 15 and 16 ; and leucovorin 20 mg / m(2 ) on days 1 , 2 , 15 and 16 , every 4 weeks . - pubmed
Imaging was performed on a clinical 1.5 Tesla scanner , using conventional balanced gradient echo sequences ( SSFP ) , T2 * -weighted sequences , and IRON - imaging . - pubmed
To this end , a series of mononuclear Mn ( III ) complexes was chosen for which the structures have been resolved by X - ray diffraction and the zfs parameters have been accurately determined by high - field EPR spectroscopy . - pubmed
Despite covering dynamic correlation contributions to the transition energies , NEVPT2 does not lead to large improvements in the results as the excitation energies of the Mn ( III ) d - d transitions are already fairly accurate at the SA - CASSCF level . - pubmed
The expression of this major transcript , namely PDCD4 ( programmed cell death gene 4 ) , was measured in beta and ductal cells after stimulation with the incretin hormone glucagon - like peptide 1 , mitogenic insulin , the thiazolidinedione rosiglitazone , and by high glucose concentrations . - pubmed
RESULTS : The expression of the Pdcd4 gene in pancreatic beta and ductal cells was found to be stimulated in a comparable manner by either glucagon - like peptide 1 , insulin , and by high glucose concentrations . - pubmed
Furthermore , the thiazolidinedione rosiglitazone specifically upregulates Pdcd4 gene expression in beta cells in a time - dependent manner . - pubmed
HO-2 oxidizes heme to CO and biliverdin ; the latter is reduced to bilirubin by biliverdin reductase ( BVR ) . - pubmed
In addition , HO-2 is a redox - sensitive K / Ca ( 2 )-associated protein , and BVR is an S / T / Y kinase . - pubmed
This is the first reporting of regulation of HO-2 by BVR and that their coordinated increase in isolated myocytes and intact heart protects against cardiotoxicity of beta - adrenergic receptor activation by isoproterenol ( ISO ) . - pubmed
In isolated cardiomyocytes , experiments with cycloheximide , proteasome inhibitor MG-132 , and siBVR suggested BVR - mediated stabilization of HO-2 . - pubmed
Perfusion of the heart with the inhibitory peptide blocked ISO - mediated BVR activation and augmented apoptosis ; conversely , perfusion with the activating peptide inhibited apoptosis . - pubmed
EGF also induces tyrosine phosphorylation of Toca-1 and interactions with N - WASP and Abi1 . - pubmed
Interest in these enzymes has increased in recent years due to hydrogen fuel initiatives and concerns over development of new methods for CO2 sequestration . - pubmed
One Ni - Fe enzyme called carbon monoxide dehydrogenase ( CODH ) is a key player in the global carbon cycle and carries out the interconversion of the environmental pollutant CO and the greenhouse gas CO2 . - pubmed
AIM : To investigate the effect of acetylcholine ( ACh ) on the cytotoxicity of natural killer ( NK ) cells and to explore the receptor mechanisms involved in the effect . - pubmed
The various concentrations of ACh , cholinergic receptor agonists or antagonists were added to the cultures , respectively according to distinct experimental purposes . - pubmed
The inhibitory effect of ACh on NK cell cytotoxicity was mimicked by pilocarpine , an agonist of muscarinic receptor , and by nicotine , an agonist of nicotinic receptor , at all applied concentrations ( 10(-10 ) - 10(-6 ) mol / L ) . - pubmed
Muscarinic receptor antagonist atropine blocked the inhibitory effect of ACh on the cytotoxicity of NK cells . - pubmed
Nevertheless , tubocurarine , an antagonist of nicotinic receptor , had no blocking effect on the suppression of NK cell cytotoxicity by ACh . - pubmed
CONCLUSION : ACh results in an inhibition of the cytotoxicity of NK cells , and this inhibition is realized mainly through M and N1 cholinergic receptor . - pubmed
In addition , ET-1 evoked the release of Ca ( 2 + ) which is a central regulator of oligodendrocyte differentiation . - pubmed
Apoptosis , total antioxide capacity ( T - AOC ) , acetylcholine esterase ( AchE ) and choline acetyl transferase ( ChAT ) activities in the hippocampus and the cortex were investigated . - pubmed
YXQNG at both doses , but not rivastigmine , had significant reduction in apoptosis , and significant increases in T - AOC and ChAT activity in both the hippocampus and the cortex . - pubmed
Unlike rivastigmine , neither dose of YXQNG showed significant reduction in AchE activity . - pubmed
Poly ( ADP - ribose ) polymerase-1 ( PARP-1 ) is a critical enzyme activated to facilitate DNA repair using nicotinamide adenine dinucleotide ( NAD + ) as a substrate . - pubmed
However , in the presence of severe oxidative stress and DNA damage , PARP-1 overactivation may ensue , depleting cells of NAD + and ATP , killing them by metabolic catastrophe . - pubmed
Selenium glycinate supplementation increases blood glutathione peroxidase activities and decreases prostate - specific antigen readings in middle - aged US men . - pubmed
Two concepts are often currently applied to selenium in adult men in the United States : Intake is generally enough to maximize blood glutathione peroxidase activities . - pubmed
The hypothesis was confirmed , in that selenium supplementation raised activities for erythrocyte and plasma glutathione peroxidase as well as lowered values for plasma prostate - specific antigen . - pubmed
Cyclooxygenase-2 ( Cox-2 ) is an inducible enzyme involved in the conversion of arachidonic acid to prostaglandin and other eicosanoids . - pubmed
Several experimental and clinical studies have established potent anti - cancer activity of NSAID ( Non - steroidal anti - inflammatory drugs ) and other Cox-2 inhibitors such as celecoxib . - pubmed
The average release of mitochondrial glutamate dehydrogenase as indicator of cell membrane damage decreased after addition of glycine . - pubmed
Meta - analysis : the diagnostic accuracy of lactose breath hydrogen or lactose tolerance tests for predicting the North European lactase polymorphism C / T-13910 . - pubmed
AIM : To perform a meta - analysis of available studies that compares the north - European genetic polymorphism C / T-13910 with the lactose breath hydrogen and the lactose tolerance tests , to determine their ability to predict geno / phenotype relationships . - pubmed
METHODS : An electronic database of the literature as well as individual references in articles were searched with the theme of genetics of lactase and comparisons with breath or lactose tolerance tests were carried out . - pubmed
While the introduction of HER2 - targeted therapies , such as the monoclonal antibody trastuzumab and the small - molecule tyrosine kinase inhibitor lapatinib , has significantly improved outcomes in HER2 + breast cancer compared with previously available therapies , use of these targeted therapies is often limited by the development of drug resistance and tolerability issues . - pubmed
Recently , several agents have demonstrated promising activity in HER2 + metastatic breast cancer , either as monotherapy or in combination therapy , including the tyrosine - kinase inhibitors neratinib ( HKI-272 ) and afatinib ( BIBW-2992 ) and the anti - HER2 monoclonal antibodies pertuzumab and trastuzumab - DM1 ( T - DM1 ) . - pubmed
Fasting serum concentrations of glucose ( P<.05 ) and insulin ( P<.0001 ) , and homeostasis model assessment index ( P<.0001 ) , were all higher in the low adiponectin group than in the normal adiponectin group . - pubmed
OBJECTIVE : Our previous study identified Threonine 161 ( Thr-161 ) , located in the second intracellular loop of the delta - opioid receptor ( DOR ) , as the only consensus phosphorylation site for cyclin - dependent kinase 5 ( Cdk5 ) . - pubmed
The aim of this study was to assess the function of DOR phosphorylation by Cdk5 in complete Freund 's adjuvant ( CFA)-induced inflammatory pain and morphine tolerance . - pubmed
RESULTS : Both the total amount and the surface localization of pThr-161 - DOR were significantly enhanced in the ipsilateral DRG following CFA injection . - pubmed
We report that formation of peroxynitrite ( PN ) in response to activation of nitric oxide synthases and NADPH oxidase in spinal cord contributes to neuropathological changes through two mechanisms . - pubmed
Targeting PN with PN decomposition catalysts ( PNDCs ) not only blocked the development of paclitaxel - induced neuropathic pain without interfering with antitumor effects , but also reversed it once established . - pubmed
The improved chemical design of SRI6 and SRI110 also affords selectivity for PN over other reactive oxygen species ( such as superoxide ) . - pubmed
Our findings identify PN as a critical determinant of CIPN , while providing the rationale toward development of superoxide - sparing and " PN - targeted " therapeutics . - pubmed
Discovery of XL413 , a potent and selective CDC7 inhibitor . - pubmed
CDC7 is a serine / threonine kinase that has been shown to be required for the initiation and maintenance of DNA replication . - pubmed
In this paper , we disclose the discovery of a potent and selective CDC7 inhibitor , XL413 ( 14 ) , which was advanced into Phase 1 clinical trials . - pubmed
The full - length cDNA of Ec - TRX was comprised of 767bp with a 327bp open reading frame that encodes a putative protein of 108 amino acids . - pubmed
The PSAP data were used to infer the mass of light absorbing carbon ( LAC ) and the daily mean varied from 0.1 to 6.8 mug m-3 . - pubmed
VEGF regulates various endothelial cellular processes , including cell migration , proliferation , and survival , through the serine threonine protein kinase Akt . - pubmed
Graphene nanosheets ( GNS ) were employed as an air electrode for a sodium - air battery ( SAB ) . - pubmed
The mean age and duration of alcohol use were 47.9 + -7.4 and 16.1 + -5.2 years , respectively . - pubmed
With the protection of Methylprednisolone , the upregulation of PUMA and p53 expression as well as the decrease of MDM2 and SirT2 in spinal cord were partially rescued in western bolt analysis . - pubmed
This association remained statistically significant for glucose , total cholesterol , and low - density lipoprotein after controlling for confounding factors in multivariate analysis . - pubmed
The protein expression of UBC9 , as compared with other biomarkers , showed an inverse correlation with steroid hormones ( ER , progesterone ( PgR ) ) , BRCA1 , p27 , p21 and MDM4 , and a positive correlation with triple negative , basal cytokeratins ( CK14 and CK5 / 6 ) , epidermal growth factor receptor ( EGFR ) , basal - like breast cancer phenotype , p53 , phosphoinositide-3 - kinases ( PI3KCA ) , placental cadherin , ( P - cadherin ) and BRCA1 regulators ( metastasis tumor antigen-1 ( MTA1 ) . - pubmed
Track - etched polyethylene terephthalate ( PET ) membranes with previously introduced aliphatic C - Br groups as initiator on the pore surface were used to prepare first a functional polymer scaffold , grafted poly ( methacrylic acid ) , via surface - initiated atom transfer radical polymerization ( SI - ATRP ) . - pubmed
After template protein ( IgG ) binding to the scaffold , UV - initiated cross - linking copolymerization of acrylamide and methylenebisacrylamide ( MBAA ) as second step lead to a grafted MIP hydrogel layer . - pubmed
The influences of the three independent parameters , scaffold chain length by SI - ATRP time , degree of cross - linking of the MIP layer by MBAA content , and grafted MIP layer thickness by UV irradiation time , were studied to optimize protein binding capacity and selectivity . - pubmed
The main inhibitory neurotransmitter , GABA , acts on both ligand - gated and G protein - coupled receptors , the GABAA / C and GABAB receptors , respectively . - pubmed
Low concordance and resistance mutation emergence in the HIV protease gene among circulating and cell - associated viruses at viral replication episodes during darunavir / ritonavir monotherapy . - pubmed
Because Eotaxin-3 - EGFP and ssEGFP ( small soluble cargo proteins ) were largely depleted from post - fusion WPBs , we studied these molecules in cells preincubated in the weak base NH4Cl which caused WPB alkalinisation and rounding similar to that produced by plasma membrane fusion . - pubmed
MT plays a critical role in zinc sequestration in the liver suggesting its therapeutic effect would be mediated by zinc . - pubmed
Zinc supplementation was introduced 4 weeks after BDL surgery via intragastric administration once daily for 2 weeks resulting in a significant reduction in the collagen deposition in the liver and an increase in the survival rate . - pubmed
Furthermore , zinc suppressed gene expression of alpha - SMA and collagen I and enhanced the capacity of collagen degradation , as determined by the increased activity of total collagenases and elevated mRNA and protein levels of MMP13 . - pubmed
Therefore , the results demonstrate that zinc supplementation suppresses BDL - induced liver fibrosis through both inhibiting collagen production and enhancing collagen degradation . - pubmed
Compared with ACSF - exposed animals , SCI - elevated [ H2O2 ] significantly increased intracellular protein and DNA oxidation by threefold and MLP by eightfold in neurons , respectively . - pubmed
H2O2 also significantly increased cell loss and the numbers of terminal deoxynucleotidyl transferase ( TdT)-mediated deoxyuridine triphosphate-(dUTP)-biotin nick end labeling ( TUNEL)-positive and active caspase-3 - positive neurons by 2.3- , 2.8- , and 5.6 - fold compared to ACSF controls , respectively . - pubmed
Our results directly and unequivocally demonstrate that SCI - elevated [ H2O2 ] contributes to post - SCI MLP , protein , and DNA oxidation to induce cell death . - pubmed
Therefore , we conclude that ( 1 ) the role of H2O2 in secondary SCI is pro - oxidation and pro - cell death , ( 2 ) apoptosis is a pathway for SCI - elevated [ H2O2 ] to induce cell death , ( 3 ) caspase activation is a mechanism of H2O2 - induced apoptosis after SCI , and ( 4 ) MnTBAP treatment significantly decreased H2O2 - induced oxidation , cell loss , and apoptosis to the levels of ACSF controls , further supporting MnTBAP 's ability to scavenge H2O2 by in vivo evidence . - pubmed
The dopamine receptor sensitization is followed , after imipramine discontinuation , by an opposite phenomenon ( dopamine receptor desensitization ) and an increased immobility time ( depression ) in the forced swimming test of depression . - pubmed
The molecular architecture of PaMTH1 and the mechanism of the methyl transfer reaction remain unknown . - pubmed
Here , we report the crystal structures of PaMTH1 apoenzyme , PaMTH1 - SAM ( co - factor ) , and PaMTH1 - S - adenosyl homocysteine ( by - product ) co - complexes refined to 2.0 , 1.9 , and 1.9 A , respectively . - pubmed
Furthermore , using NMR , mass spectrometry , and site - directed active site mutagenesis , we show that PaMTH1 catalyzes the transfer of the methyl group from SAM to one hydroxyl group of the myricetin in a cation - dependent manner . - pubmed
genitalium with macrolide resistance - associated 23S rRNA mutations and fluoroquinolone resistance - associated parC alterations were 47.1 % and 36.8 % , respectively . - pubmed
In the era of increasing resistance to antibiotics , the efficacy of fluoroquinolone prophylaxis and standard treatment of febrile neutropenia have been questioned . - pubmed
Post - hoc , observed - case analysis included pooled data from six studies on 40 - 160 mg / day lurasidone ; two studies included active comparators ( 2 - 6 mg / day risperidone or 200 - 800 mg / day quetiapine XR ) . - pubmed
AIM : A contralateral breast cancer ( CBC ) is today treated as an independent primary tumour , although recent data suggest risk and prognosis of CBC to be influenced by characteristics of and treatment given for the first tumour ( BC1 ) . - pubmed
Crayfish visceral and tail tissue , as well as water - deployed LDPE , were extracted and analyzed for 62 PAHs using GC - MS / MS . - pubmed
Polydatin Protecting Kidneys against Hemorrhagic Shock - Induced Mitochondrial Dysfunction via SIRT1 Activation and p53 Deacetylation . - pubmed
Beneficial effect of PD was abolished partially when the SIRT1 inhibitor Ex527 was added . - pubmed
The transepithelial electrical resistance , MTT ( 3 -( 4,5 - dimethyl-2 - thiazolyl )- 2,5 - diphenyltetrazolium bromide ) assay , and lactate dehydrogenase ( LDH ) release rate were investigated . - pubmed
The MTT assay revealed that the cells recovered their mitochondrial dehydrogenase activity without proliferation . - pubmed
These results suggest that the damage by MCFA compounds is recoverable and that the recovery can be assessed by an MTT assay , but that the LDH - release behavior is different among the enhancers . - pubmed
Isoliquiritigenin suppresses tumor necrosis factor - alpha - induced inflammation via peroxisome proliferator - activated receptor - gamma in intestinal epithelial cells . - pubmed
A signal transduction study revealed that ISL significantly attenuates TNF - alpha - mediated JNK phosphorylation . - pubmed
ISL - induced ERK1 / 2 phosphorylation was associated with PPARgamma expression . - pubmed
Collectively , ISL - induced PPARgamma mediated , at least partially , the suppression of intestinal inflammation . - pubmed
We precisely controlled the MOS interface for diamond by wet annealing and fabricated p - channel and planar - type MOSFETs with phosphorus - doped n - type body on diamond ( 111 ) substrate . - pubmed
Detailed microscopic analysis shows that the NTE relies on strong intralayer Ti - O covalent bonding and weaker interlayer interactions , which is in contrast to conventional NTE mechanisms for perovskites , such as rigid - unit modes , structural transitions , and electronic or magnetic ordering . - pubmed
Birds fed the lowest level of dietary lysine ( 1.016 % ) showed a lower expression of genes such as NADH dehydrogenase subunit I ( ND1 ) , cytochrome b ( CYTB ) and cytochrome c oxidase subunits I ( COX I ) , II ( COX II ) and III ( COX III ) , displaying the worst performance and body protein deposition . - pubmed
Several hypotheses can be proposed for the molecular mechanisms of the protection conferred by DRB1 * 13 , some of which can explain the dominant effect of DRB1 * 13 molecules over the susceptible alleles , but the actual protective function of DRB1 * 13 requires further study . - pubmed
Arsenic accumulation and speciation were analyzed using inductively coupled plasma - mass spectrometry ( ICP - MS ) . - pubmed
Identification of residues that control Li + versus Na + dependent Ca2 + exchange at the transport site of the mitochondrial NCLX . - pubmed
BACKGROUND : The Na +/ Ca2 +/ Li + exchanger ( NCLX ) is a member of the Na+/Ca2 + exchanger family . - pubmed
The metabolites showed positive correlations with total- and LDL - cholesterol ( r ranged from 0.13 to 0.57 ) . - pubmed
When additionally adjusting for total- , LDL- and HDL - cholesterol , triglycerides and C - reactive protein , acyl - alkyl - phosphatidylcholine C36:3 and diacyl - phosphatidylcholines C38:3 and C40:4 remained associated with risk of MI . - pubmed
In this study , a thermosensitive butane diisocyanate ( BDI)-collagen hydrogel ( BC hydrogel ) was designed as an injectable cell delivery carrier of tendon stem / progenitor cells ( TSPCs ) for tendon tissue engineering . - pubmed
It was significant correlation between EMAP - II and HbAc1 , blood glucose , total cholesterol , LDL , triglycerides and inverse correlation between EMAP - II and HDL , endotheliumdependent dilatation . - pubmed
The revealed change of EMAP - II serum level reflects an endothelial dysfunction in type 1 diabetes , alteration of carbohydrate and lipid metabolism could influence of this pathway . - pubmed
OBJECTIVE : To evaluate the effects of CCB on EF we have performed a meta - analysis of available data from randomized and placebo - controlled or other treatment - controlled clinical studies encompassing effects of CCB on EF , as measured by flow - mediated dilation ( FMD ) of the brachial artery . - pubmed
CCB improved FMD more pronounced than thiazide diuretics - TD ( 3 studies , 157 participants , WMD=2.08 % , 95 % CI=0.35 - 3.80 % ; P=0.02 ) . - pubmed
Oppositely , ACE inhibitors ( ACEI ) and angiotensin receptor blockers ( ARB ) notably improved FMD if compared to CCB ( CCB vs. ACEI : 5 studies , 533 participants , WMD = -1.62 % , 95 % CI = -2.74 % to -0.50 % ; P=0.005 ; and CCB vs. - pubmed
Evidence for the synthesis of an unusual high spin ( S = 7/2 ) [ Cu-3Fe-4S ] cluster in the radical - SAM enzyme RSAD2 ( viperin ) . - pubmed
We demonstrate the synthesis and spectroscopic characterization of an unusual high spin ( S = 7/2 ) [ Cu-3Fe-4S ] cluster in a member of the radical - SAM enzymes . - pubmed
52.6 % ( n = 72 ) of them were treated with dementia medication : AchEIs 16.1 % ( n = 22 ) , memantine 9.5 % ( n = 13 ) , antidepressants 23.4 % ( n = 32 ) , antipsychotic medication 9.5 % ( n = 13 ) , tranquilisers 16.8 % ( n = 23 ) and anticonvulsant drugs 6.6 % ( n = 9 ) . - pubmed
The enzymatic hydrolysis of chitin , one of the most abundant carbohydrates in nature , is achieved by chitinases , enzymes of increasing importance in biomedicine and industry . - pubmed
Unlike most retaining glycosidases , family GH18 chitinases follow a substrate - assisted mechanism in which the 2 - acetamido group of one N - acetylglucosamine monomer , rather than a basic residue of the enzyme , reacts with the sugar anomeric carbon , forming an intermediate that has been described as an oxazolinium ion . - pubmed
The interpretation of these results as an evidence for the RO5263397 anticompulsive - like action , however , should be taken with caution because the drug also influenced the drinking behavior and only weakly affected the other parameters of SIP used to reveal the potential anticompulsive - like effects of drugs . - pubmed
The chromatographic separation was achieved on a Waters HSS T3 column under gradient elution with acetonitrile and water containing formic acid as the mobile phase . - pubmed
PSI has the potential to generate reactive oxygen species and be oxidatively inactivated by the reactive oxygen species . - pubmed
It has been reported that PSI photoinhibition does not occur as long as the reaction - center chlorophyll ( P700 ) remains oxidized , even in excess light conditions . - pubmed
PSI photoinhibition correlated linearly with decreases in effective quantum yield of PSII and nonphotochemical quenching ; however , the photosynthetic CO2 assimilation rate was less affected , as exemplified by ~50 % of the normal CO2 assimilation rate maintained with an 80 % loss in PSI photochemical activity . - pubmed
Based on these results , we propose that the stoichiometric quantity of PSI is optimized to induce P700 oxidation for dissipating excess light energy in PSI , thus avoiding inhibition of photosynthetic CO2 assimilation caused by PSI photoinhibition . - pubmed
Simple linear regression models revealed that ammonia - oxidizing archaea ( AOA ) contributed much to N2O emission ( R2 = 0.77 ) . - pubmed
Additionally , the R2 coefficient of linear regression was 0.87 between nosZ genes and N2O emission rates in alpine meadow . - pubmed
Novel antimycobacterial C-21 amide derivatives of the antibiotic fusidic acid : synthesis , pharmacological evaluation and rationalization of media - dependent activity using molecular docking studies in the binding site of human serum albumin . - pubmed
The fusidic acid ethanamides were , generally , the most potent of the analogues evaluated for antimycobacterial activity ( MIC90 & lt ; 10 muM ) in the MB7H9 / CAS medium . - pubmed
The lack of activity in the MB7H9 / ADC medium was supported by strong binding interactions in the fusidic acid binding site of the human serum albumin ( HSA ) protein . - pubmed
Doxorubicin and liposomal doxorubicin induce senescence by enhancing nuclear factor kappa B and mitochondrial membrane potential . - pubmed
KEY FINDINGS : Based on the results , the highest doses of Dox and Ldox ( 0.75 and 0.1 mg / kg / BW respectively ) significantly increased the level of inflammatory markers and according to other factors especially p53 and SA beta - gal expression , both were able to induce senescence but the changes in Ldox were less tangible than the Dox . - pubmed
Identification of Aloperine as an anti - apoptotic Bcl2 protein inhibitor in glioma cells . - pubmed
In addition , molecular docking demonstrated that ALO expectedly docks into the active pocket of the Bcl2 protein , suggesting Bcl2 as a direct target of ALO . - pubmed
In addition , Bcl-2 overexpression could rescue ALO - induced Bcl-2 inhibition and suppress pro - apoptotic effects in glioma cells . - pubmed
Conclusion : Taken together , these findings suggest that the natural agent ALO effectively enhances apoptosis by acting as a potential Bcl2 inhibitor in human glioma cells . - pubmed
In this study , three - dimensional quantitative structure - activity relationship ( 3D - QSAR ) models of PPARalpha receptor ( CoMFA-1 , q2 = 0.636 , r2 = 0.953 ; CoMSIA-1 , q2 = 0.779 , r2 = 0.999 ) and PPARdelta receptor ( CoMFA-2 , q2 = 0.624 , r2 = 0.906 ; CoMSIA-2 , q2 = 0.627 , r2 = 0.959 ) were successfully constructed using 35 triazolone ring derivatives . - pubmed
In the present study , we developed an environmentally friendly and convenient method to decorate the wood fiber / polypropylene ( WF / PP ) composite board . - pubmed
We used chlorinated polypropylene ( CPP ) as an intermediate film to laminate wood veneer to WF / PP composite boards by hot - pressing at 110 C and then cooling down . - pubmed
Vitamin C is incorporated into the cerebrospinal fluid ( CSF ) through choroid plexus cells . - pubmed
While the transfer of vitamin C from the blood to the brain has been studied functionally , the vitamin C transporter , SVCT2 , has not been detected in the basolateral membrane of choroid plexus cells . - pubmed
The effect of energy level alignment between the hole transport layer ( HTL ) and active layer in PbS quantum dot ( QD ) solar cells was investigated . - pubmed
Our results indicated that DEHP is cytotoxic ; it induces the overexpression of Hsp70 protein and causes oxidative damage through the generation of free radicals leading to lipid peroxidation induction and the increase of superoxide dismutase ( SOD ) and catalase ( CAT ) activities . - pubmed
In addition , cell treatment with DEHP resulted in a glutathione ( GSH ) content decrease and a decrease in the glutathione reductase ( GR ) activity . - pubmed
As new evidence provided in this study , we demonstrated that the DEHP affected the two enzymes ' activities of the oxidative phase of the pentose phosphate pathway : Glucose-6 - phosphate dehydrogenase ( G6PD ) and 6 - phosphogluconate dehydrogenase ( 6PGD ) . - pubmed
This leads to a decrease in the level of NADPH used by the GR to maintain the regeneration of the reduced GSH . - pubmed
PBS and HPBS are a type of biological products detected on the C - S bond cleavage step through various oxidative biodesulfurization ( OBDS ) pathways , and are useful synthetic intermediates and fine chemicals . - pubmed
A stabilized power conversion efficiency ( PCE ) of 16.1 % under simulated AM 1.5 G 1 sun illumination with an aperture of 1.00 cm2 was achieved for PSCs using an additive - free spiro - OMeTAD layer cast from PC . - pubmed
Molecular mechanism for bidirectional regulation of CD44 for lipid raft affiliation by palmitoylations and PIP2 . - pubmed
The process is controlled by two different pathways : On the one hand palmitoylation keeps CD44 in lipid raft domains and disables the linking to the cytoplasmic adaptor , whereas on the other hand , the presence of phosphatidylinositol-4,5 - biphosphate ( PIP2 ) lipids accelerates the formation of the CD44 - adaptor complex . - pubmed
The molecular mechanism explaining how CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of PIP2 remains , however , elusive . - pubmed
We observe a delicate balance between the influence of the palmitoylations and the presence of PIP2 lipids : whereas the palmitoylations of CD44 increases the affinity for raft domains , PIP2 lipids have the opposite effect . - pubmed
We find that the presence of PIP2 lipids allows CD44 and FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity . - pubmed
OBJECTIVE : Combined methylmalonic acidemia and homocysteinemia is a genetically heterogeneous disorder of cobalamin ( cbl ; vitamin B12 ) metabolism , which consists of five subtypes including cblC , cblD , cblF , cblJ , and cblX deficiencies . - pubmed
Changes in the osmolality and level of angiotensin II ( ANG II ) are important peripheral signals modulating appropriate central sympathetic output and maintaining a normal arterial pressure during high salt intake . - pubmed
The median preoptic nucleus ( MnPO ) receives reciprocal inputs from the subfornical organ ( SFO ) and organum vasculosum of the lamina terminalis ( OVLT ) , the circumventricular organs that have been shown to be necessary in multiple central effects of changes in the osmolality and circulating ANG II directed toward the maintenance of sodium and water homeostasis . - pubmed
Femoral venous catheters were also implanted to collect blood for the measurements of plasma osmolality and sodium concentration , as well as plasma renin activity . - pubmed
Identification of a novel homozygous variant in the alkaline phosphate ( ALPL ) gene associated with hypophosphatasia . - pubmed
IgG and IgG2a titers elicited by the Ace - DEX MP platform were compared to titer levels elicited by MSP2 adsorbed to an FDA - approved alum adjuvant , MSP2 alone , and PBS alone . - pubmed
Both adsorption of MSP2 to Ace - DEX MPs and to alum elicited antibody responses in vivo , but only the formulation containing Ace - DEX MPs was able to elicit a significant Th1 - biased response needed to combat the intracellular pathogen . - pubmed
As such , MSP2 adsorbed to Ace - DEX MPs demonstrates promise as a malaria vaccine . - pubmed
Compared with the placebo , probiotic and selenium co - supplementation resulted in a significant elevation in total antioxidant capacity ( beta 91.09 mmol / L ; 95 % CI , 35.89 , 146.30 ; P = 0.002 ) and total glutathione ( beta 96.50 mumol / L ; 95 % CI , 26.13 , 166.87 ; P = 0.008 ) and a significant reduction in high - sensitivity C - reactive protein levels ( beta - 1.44 mg / L ; 95 % CI , - 2.22 , - 0.66 ; P = 0.001 ) . - pubmed
Additionally , co - supplementation significantly decreased fasting glucose ( beta - 7.40 mg / dL ; 95 % CI , - 10.15 , - 4.64 ; P < 0.001 ) , insulin levels ( beta - 1.46 muIU / mL ; 95 % CI , - 2.35 , - 0.57 ; P = 0.002 ) , and homeostasis model of assessment - insulin resistance ( beta - 0.51 ; 95 % CI , - 0.72 , - 0.29 ; P < 0.001 ) and a significant increase in quantitative insulin sensitivity check index ( beta 0.01 ; 95 % CI , 0.006 , 0.01 ; P < 0.001 ) compared with the placebo . - pubmed
It was found that it amplified the induction of liver tyrosine aminotransferase by dexamethasone markedly with doses of dexamethasone that caused minimal enzyme induction , but had little effect with doses that caused maximal induction . - pubmed
These results and the finding that actinomycin D inhibited the amplification indicate that the amplifier does not act on the translational level of enzyme induction . - pubmed
It was found that the amplifier increased both incorporation of [ 3H ] dexamethasone into the cytosol and binding of [ 3H ] dexamethasone of cytosol protein and that it decreased decay of the [ 3H ] dexamethasone - protein complex . - pubmed
sIg+ lymphocytes detected by immunofluorescence and by 51Cr release cytotoxicity showed similar frequency and adherence properties . - pubmed
Increased precipitability of IgG and C4 was seen in a great percentage ( 80 % ) of pathological sera giving positive PEG precipitation without direct influence of IgG , IgM , C1q , C3 and C4 serum levels . - pubmed
The C1q binding test could be replaced by the more direct and simple evaluation of the amount of C4 precipitated with IgG by 3.5 % PEG . - pubmed
The lung is the site of a number of reactions affecting vasoactive substances : hydrolysis of angiotensin I , bradykinin and ATP , occurring at the surface of endothelial cell ; uptake and intracellular metabolism of 5 - hydroxytryptamine and norepinephrine ; and intracellular oxidation of prostaglandins . - pubmed
Pretreatment of granulosa cells with activin , but not FSP , for 24 h increased the responsiveness of cells to FSH ( 20 ng / ml ) and LH ( 40 ng / ml ) shown by increases in aromatase activity , progesterone , and immunoreactive inhibin production over and above control in a manner which depended upon activin doses . - pubmed
Absorption and circular dichroism spectra of stable enzyme - substrate intermediates of aspartate aminotransferase were recorded at subzero temperatures ( down to -65 degrees C ) in the cryosolvent water / methanol . - pubmed
The intermediates were formed either between the pyridoxal form of the enzyme and its amino acid substrates , or between the pyridoxamine form and its oxo acid substrates . - pubmed
Accumulation of the ketimine in the reverse direction would indicate that the proton abstraction at C4 ' is rate - limiting in this half - reaction . - pubmed
on the therapeutic level : the attacks resist barbiturate and benzodiazepin treatment in 80 % of the cases , but the attacks diminish as the diabetes is brought under control through insulin treatment . - pubmed
There is evidence that a functional deficit of serotonin / noradrenaline and/or of their precursors L - tryptophan ( L - TRP )/ tyrosine and disorders in the hypothalamic - pituitary - adrenal ( HPA ) axis are linked to the pathophysiology of severe depressions . - pubmed
Above indications were all decreased ; especially both the thymus weight and the thymus ADA activity were markedly decreased in the zinc deficient group . - pubmed
After zinc supplementation for one month , all the body weight , weight of the thymus and spleen , and ADA activity of the spleen were recovered to the control group 's level , while ADA activity of the thymus was 33.03 % higher than that of the control group . - pubmed
Our data suggested that zinc was not only an important trace element in maintaining the growth of the body , the development of the thymus and spleen , but it may be an important agent to activate the ADA or to prompt synthesis of ADA . - pubmed
specificity of the 5 laboratory values AP , GGT , GPT , GOT and bilirubin was 22 % resp . - pubmed
The diagnostic value determined by their Chi2 - value was AP greater than GPT greater than bilirubin greater than GOT greater than GGT . - pubmed
The combination of AP , GPT and bilirubin with the result of abdominal sonography in a logistic discriminant function led to a correct a posteriori classification of all patients . - pubmed
The serine esterase affinity label diisopropyl fluorophosphate reacts strongly with granzymes A and D , to a lesser extent with B , E , F , G , and H , and not at all with C and F. - pubmed
Quaternary forms of classical muscarinic antagonists distinguish subpopulations of muscarinic receptors : correlation with gallamine - defined subpopulations . - pubmed
Under the same conditions , the inhibition by N - methylatropine ( NMA ) and N - methylscopolamine ( NMS ) of the binding of [ 3H ] QNB is consistent with the presence of subpopulations of receptors that differ greatly in affinities toward these quaternary ligands . - pubmed
Thyroxine , triiodothyronine , thyroglobulin , hypophyseal thyrotrophic and gonadotrophic hormone , prolactin , cortisol and corticotrophin concentrations in the peripheral blood of 475 healthy females were measured by radioimmunoassay at different times of their arrival to the North . - pubmed
The role of adrenergic receptors in Ni2 + -induced coronary vasoconstriction . - pubmed
The experiments on both models showed that ( i ) alfa - adrenergic blockade by phenoxybenzamine or phentholamine caused only partial depression of Ni2 + -induced coronary vasoconstriction : ( ii ) beta - adrenergic receptor blockade by propranolol totally prevented Ni2 + -action , and ( iii ) Ni2 + ( 1 microM ) caused significant inhibition of coronary vasodilatation induced by isoproterenol . - pubmed
The experimental results indicate that alfa - adrenoceptors play minor role ( if any ) in the coronary action mechanism of Ni2 + but it may be mediated by beta - adrenergic mechanisms . - pubmed
Nickel was found to alter the reactivity of coronary beta - adrenoceptors suggesting a possible modulatory role of this trace metal in coronary adrenergic mechanisms . - pubmed
Finally , we show that high MBA gels are also effective in resolving mixtures of oligoribonucleotides such as those produced by T1 ribonuclease digestion of small RNAs . - pubmed
Comparison of gonadotropin releasing hormone and human chorionic gonadotropin and effects of progestogen and estrogen . - pubmed
The demethylating enzyme activity is inducible by phenobarbital ( PB ) , not by 3 - methylcholanthrene ( 3 - MC ) . - pubmed
Pretreatment of animals with PB plus 3 - MC resulted in reduced inducibility compared with PB - pretreatment alone . - pubmed
Probably this is not due to an inhibition of PB - induction by 3 - MC , but effected by PB residues in the liver tissue and in the incubation mixture . - pubmed
The effector cells are insensitive to anti - Thy 1 antibody and complement treatment , are not H-2 restricted , do not adhere to plastic and are unaffected by silica or carrageenan . - pubmed
The treatment results were evaluated by thyroid scintigraphy and sonography , by triiodothyronine , thyroxine , and thyrotropin assays , and by a thyrotropin - releasing hormone stimulation test 6 months after the end of treatment . - pubmed
Growth hormone ( GH ) suppresses its own secretion by stimulating somatostatin ( SRIF ) release . - pubmed
Our results show that alpha - amanitin blocks cells specifically in G1 , irrespective of the concentration within the range of 3 to 30 micrograms / ml . - pubmed
This suggests that cells exposed to alpha - amanitin are able to complete their cell cycle because sufficiently high levels of mRNA are present in S / G2 due to basal level transcription , or are left from preceding cell cycles . - pubmed
Serum testosterone concentration was higher before the race at altitude than at sea level ( 19 % , P & lt ; 0.02 ) , and 30 min after the race growth hormone ( GH ) concentration was higher at sea level than at moderate altitude ( P & lt ; 0.002 ) . - pubmed
This was followed by in situ hybridization using 35S - labeled riboprobes for PRL and GH mRNA . - pubmed
Incorporation of these fatty acids leads to profound changes in membrane fatty acid composition which in turn may alter the activity of transmembrane receptor / effector systems . - pubmed
It is suggested that beta - adrenoreceptor number , subtype and adenylyl cyclase stimulation may be regulated by alterations in membrane fatty acid composition as a result of changes in the levels of polyunsaturated fatty acids and alterations in eicosanoid production . - pubmed
The most evaluated regimens include dual therapy with a proton pump inhibitor and either amoxicillin or clarithromycin , and bismuth - based triple therapy with metronidazole and tetracycline . - pubmed
Moreover , combinations of a proton pump inhibitor and clarithromycin show more predictable and higher eradication rates than amoxicillin combinations . - pubmed
Actual pentamidine dose delivered by Respigard II nebulizer . - pubmed
The Respigard II nebulizer system is the approved method to deliver pentamidine aerosols in the USA . - pubmed
Pentamidine solutions ( 300 mg in 6 mL water ) were nebulized continuously with the Respigard II nebulizer system until dryness ( 40 min operation ) . - pubmed
SS receptors were identified using 125I - Tyr(11)-SS and isolated pancreatic acinar membranes in vitro . - pubmed
The aim of this study was to characterize the changes in the quantitative expression of beta 2 - integrins and L - selectin detected by means of fluorochrome - conjugated monoclonal antibodies and flow cytometry on leukocytes in the systemic circulation after a major musculoskeletal trauma , i.e. - pubmed
The cells on the PPy - coated ITO plate could be kept in culture , without any significant changes in morphology and in the secretory responsiveness to acetylcholine as compared with those of the cells cultured on collagen . - pubmed
This paper describes that PPy films are applicable as a polymer - modified electrode which support the cell function without collagen . - pubmed
A combination of acyclovir and interferon therapy was started at 25 weeks . - pubmed
[ Glycogenolysis through lyase reaction and 1,5 - anhydroglucitol ] . - pubmed
The phosphorylation of a serine residue at position 133 of CREB is critical for activation of CREB . - pubmed
It is initiated by a highly local , rise in calcium ion concentrations near the cell membrane , but culminates in the activation of a specific calmodulin - dependent kinase known as CaMK IV , which is constitutively present in the neuronal nucleus . - pubmed
Certain Ca2 + entry systems ( L - type Ca2 + channels and NMDA receptors ) are able to cause mobilization of calmodulin , whereas others ( N- and P / Q - type Ca2 + channels ) are not . - pubmed
This translocation of calmodulin provides a form of cellular communication that combines the specificity of local Ca2 + signalling with the ability to produce action at a distance . - pubmed
However , all recombinant viruses contained an insertion of two amino acids between codons 68 and 69 of RT as well as an amino - acid change at codon 67 , as was seen in the serum - derived viruses . - pubmed
CONCLUSIONS : Antiretroviral therapy including zidovudine may yield replicating viruses with a two amino - acid insertion in RT in combination with amino - acid changes at codons 67 and 215 , which are highly resistant to lamivudine and stavudine on top of zidovudine and have unpredictable susceptibility to didanosine and zalcitabine despite lack of previously reported corresponding resistance - associated amino - acid changes . - pubmed
The same features were found in all four strains of the genus Fibrobacter metabolizing simultaneously glucose and cellobiose : i ) differential metabolism of glucose and cellobiose ; glucose seems preferentially used for glycogen storage and energy production , while part of cellobiose seems to be diverted from glycolysis , ii ) synthesis of cellodextrins , mainly from cellobiose not entering into glycolysis , iii ) accumulation of glucose 6 - phosphate , iv ) simultaneous presence of cellobiose phosphorylase and cellobiase activities . - pubmed
Many of axon terminals of striatonigral neurons were confirmed electron microscopically not to be in synaptic contact with SPR - like immunoreactive structures within the striatum . - pubmed
p62 is an only recently discovered protein that is a phosphotyrosine - independent ligand of the SH2 domain of p56 ( lck ) , a member of the c - src family of cytoplasmic kinases . - pubmed
Average systolic peak velocity ( ASV ) and deceleration time of diastolic flow velocity ( DDT ) significantly correlated to 1 - month A - WMSI ( r=-0.54 , P=0.007 and r=-0.62 , P=0.002 , respectively ) , and optimal cutoff values to predict viable myocardium ( defined as 1 - month A - WMSI & lt;/=2.0 ) were 6.5 cm / s for ASV and 600 ms for DDT ( sensitivity=0.79 , specificity=0.89 and sensitivity=0.86 , specificity=0.89 , respectively ) . - pubmed
Fertility was measured by serum progesterone ( P(4 ) ) concentrations and pregnancy diagnosis by rectal examination post - insemination . - pubmed
They were questioned regarding risk factors and blood samples were taken for HBsAg , anti - HBs and anti - HBc testing . - pubmed
Development of the antigen retrieval ( AR ) technique , a simple method of boiling archival paraffin - embedded tissue sections in water to enhance the signal of immunohistochemistry ( IHC ) , was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine formalin - fixed , paraffin - embedded tissues for wide application of IHC in research and clinical pathology . - pubmed
Nanoelectrospray ionization mass spectrometry ( nanoESI - MS ) and computer simulation were applied to the characterization of non - covalent interactions of [ Leu5 ]- enkephalin ( LE ) and its optical isomers , [ D - Tyr1 , Leu5 ]- enkephalin ( Y - LE ) , [ D - Phe4 , Leu5 ]- enkephalin ( F - LE ) and [ D - Tyr1 , D - Phe4 , Leu5 ]- enkephalin ( YF - LE ) . - pubmed
The dimer formation tendencies of the optical isomers of LE were evaluated by nanoESI - MS using quadruply deuterated LE ( H2N - Tyr -( 2,2 - d2 ) Gly -( 2,2 - d2 ) Gly - Phe - Leu - COOH , d4 - LE ) as an internal standard . - pubmed
It was positive in vacuole with acridine orange and acid phosphatase stain . - pubmed
Upon in vitro decidualization of endometrial stromal cells with progesterone , PAPP - A levels in CM increased nearly 9 - fold without a concomitant change in proMBP . - pubmed
In contrast to the experiments with trophoblasts , IGF - II and the IGF analogues , Leu(27 ) IGF - II , and Des ( 1 - 6 ) IGF - II , resulted in a dose - dependent decrease of PAPP - A levels in decidualized endometrial stromal CM by 70 - 90 % , and a dose - dependent increase in proMBP of 14- to 41 - fold . - pubmed
Among the most detailed mediators studied are corticotropin - releasing factor and serotonin which , via the hypothalamic - pituitary - adrenal axis and the sympathetic and parasympathetic nervous system , stimulate catecholamines and cortisol and inhibit anabolic hormones , insulin , leptin , ghrelin , including neuropeptide Y and other neuropeptides , among them the paracrine - acting cytokines . - pubmed
Cadaverine inhibition of porin plays a role in cell survival at acidic pH. - pubmed
In order to understand the physiological significance of cadaverine excretion and the inhibition of porins , we isolated an OmpC mutant that showed resistance to spermine during growth and polyamine - resistant porin - mediated fluxes . - pubmed
Here , we show that the addition of exogenous cadaverine allows wild - type cells to survive a 30 - min exposure to pH 3.6 better than cells expressing the cadaverine - insensitive OmpC porin . - pubmed
Competition experiments between strains expressing either wild - type or mutant OmpC showed that the lack of sensitivity of the porin to cadaverine confers a survival disadvantage to the mutant cells at reduced pH. - pubmed
On the basis of these results , we propose that the inhibition of porins by excreted cadaverine represents a novel mechanism that provides bacterial cells with the ability to survive acid stress . - pubmed
HHV-8 - gB possesses the Arg - Gly - Asp ( RGD ) motif , the minimal peptide region of many proteins known to interact with subsets of host cell surface integrins . - pubmed
A truncated form of gB without the transmembrane and carboxyl domains ( gBdeltaTM ) and a gBdeltaTM mutant ( gBdeltaTM - RGA ) with a single amino acid mutation ( RGD to RGA ) were expressed in a baculovirus system and purified . - pubmed
In contrast , adhesion was not blocked by the preincubation of plate - bound gBdeltaTM protein with heparin , suggesting that the adhesion is mediated by the RGD amino acids of gB , which is independent of the heparin - binding domain of gB. - pubmed
Adhesion induced by the gBdeltaTM was blocked by EDTA , thus suggesting the role of integrins in the observed adhesions . - pubmed
Inhibition of FAK phosphorylation by genistein blocked gBdeltaTM - induced FAK activation and cell adhesion . - pubmed
These included measurement of parathyroid hormone ( PTH ) or vitamin D metabolites , or receipt of calcitriol or calcium - containing phosphate binders ( CCPB ) prior to RRT . - pubmed
RESULTS : Only 3.4 % of CKD patients had their PTH assessed prior to RRT , and 0.3 % had vitamin D status measured . - pubmed
We observed no interference from anti - HBc antibody or blood components , but the assay was inhibited by very high concentrations ( & gt;1 microg / ml ; corresponding to 80 signal / cutoff ) of HBeAg . - pubmed
Solutes were separated on a C18 column with a mobile phase of methanol - acetate buffer ( pH 4.0 ) ( 80:20 ) . - pubmed
Expression profiling identified processes likely responsible for 18 - fluoro-2 - deoxy - glucose uptake and tumor localization by positron emission tomography , and a role for hypoxia - inducible factor-1 was suggested . - pubmed
[ Current ACTH and cortisone therapy ] . - pubmed
[ DESOXYGLUCOSYLTHYMINE AS AN INHIBITOR OF PYRIMIDINE - NUCLEOSIDE - PHOSPHORYLASE FROM ASCITES TUMOR CELLS ] . - pubmed
[ ON THE EFFECT OF THE ENZYME INHIBITOR TRASYLOL IN THE THERAPY AND PROPHYLAXIS OF ACUTE PANCREATITIS : REPORT BASED ON 893 CASES ] . - pubmed
Recently , a family of tyrosine kinase inhibitors , the tyrphostins , has been successfully used in models of endotoxemia , peritonitis , and hypovolemic shock . - pubmed
CONCLUSIONS : Tyrphostin Ag 556 alters spinal cord ischemia reperfusion injury by inhibiting protein kinases . - pubmed
ESI MS analysis of NAT1 treated with Br - AAF showed that a single molecule of 2 - acetylaminofluorene had been incorporated . - pubmed
Proteolysis with pepsin followed by sequencing of adducted peptides by ESI MS / MS identified the modified residue as the catalytically essential Cys-68 . - pubmed
ESI Q - TOF MS analysis of NAT1 that had been treated with bromoacetanilide resulted in identification of a monoadducted protein as the primary product and a diadducted protein as a minor product . - pubmed
Pepsin digestion of bromoacetanilide - inactivated NAT1 and sequencing by ESI MS / MS identified Cys-68 as the primary site of adduct formation . - pubmed
Additional proteolysis of the bromoacetanilide - treated NAT1 led to the identification of a second modified peptide which was adducted at Cys-44 . - pubmed
Zinc depletion resulted in a decrease in the activity levels of the CDP - diacylglycerol pathway enzymes phosphatidylserine synthase , phosphatidylserine decarboxylase , phosphatidylethanolamine methyltransferase , and phospholipid methyltransferase . - pubmed
In contrast , the activity of phosphatidylinositol synthase was elevated in response to zinc depletion . - pubmed
For the CHO1 - encoded phosphatidylserine synthase , the reduction in activity in response to zinc depletion was controlled at the level of transcription . - pubmed
This regulation was mediated through the UAS(INO ) element and by the transcription factors Ino2p , Ino4p , and Opi1p that are responsible for the inositol - mediated regulation of UAS(INO)-containing genes involved in phospholipid synthesis . - pubmed
A zrt1Delta zrt2Delta mutant ( defective in the plasma membrane zinc transporters Zrt1p and Zrt2p ) grown in the presence of zinc exhibited a phospholipid composition similar to that of wild type cells depleted for zinc . - pubmed
Determination of midazolam and its metabolite as a probe for cytochrome P450 3A4 phenotype by liquid chromatography - mass spectrometry . - pubmed
This study demonstrated the analysis of midazolam and its metabolites by liquid chromatography - mass spectrometry ( LC - MS ) with a sonic spray ionization ( SSI ) interface . - pubmed
We also investigated the effect of propofol on the metabolism of midazolam using recombinant CYP3A4 . - pubmed
Propofol competitively inhibited the metabolism of midazolam to 1 '- hydroxymidazolam by CYP3A4 . - pubmed
The EGFR , EGFRvIII expression and tyrosine phosphorylation ( Tyr - P ) levels were quantified by Western blotting . - pubmed
Docetaxel plus epirubicin as first - line chemotherapy in MBC ( KCSG 01 - 10 - 05 ): phase II trial and the predictive values of circulating HER2 extracellular domain and vascular endothelial growth factor . - pubmed
We conducted a multicenter phase II study to evaluate the efficacy and tolerability of the docetaxel plus epirubicin combination chemotherapy as first - line treatment in MBC and performed a prospective assessment of the predictive values of circulating HER2 extracellular domain ( ECD ) and vascular endothelial growth factor ( VEGF ) . - pubmed
[ Synthesis of purpure-18 - imide and study on its interaction with bovine serum albumins by fluorescence ] . - pubmed
The binding reaction between purpure-18 - imide and bovine serum albumins ( BSA ) in aqueous was studied by fluorescence and UV - Vis absorption spectra . - pubmed
In aqueous , purpure-18 - imide strongly bound BSA with molar ratio of 1:1 . - pubmed
About 40 % of hK11 is enzymatically active , whereas the rest is inactivated by internal cleavage after Arg156 ( Genbank accession no . - pubmed
Purified hK11 possesses trypsin - like activity and cleaves synthetic peptides after arginine but not lysine residues . - pubmed
The strongest inhibitor , APMSF , completely inhibited hK11 activity at a concentration of 2.5 mmol / L. Aprotinin and an hK11 - specific monoclonal antibody inhibited hK11 activity up to 40 % . - pubmed
Plasmin is a strong candidate for cleaving hK11 at Arg156 . - pubmed
The sulfide oxidase was purified to homogeneity and the molecular weight of the purified enzyme was 43 kDa when estimated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis . - pubmed
The purified sulfide oxidase can be effectively immobilized in DEAE ( diethylaminoethyl)-cellulose matrix with a yield of 66 % . - pubmed
The purified free sulfide oxidase activity was completely inhibited by 1 mM Co2 + and Zn2 + and sulfhydryl group reagents ( para - chloromercuribenzoic acid and iodoacetic acid ) . - pubmed
Empiric treatments include the use of calcium channel blockers , steroids , induced hypertension or blood pressure lowering , and rarely endovascular therapy . - pubmed
Taking into account the structural requirements for TICT - type sensor molecules , a general synthetic route to derive pH and cation - responsive pretwisted donor ( D )- donor ( D ) biphenyls ( b ) equipped with donor receptors is developed and a first model compound containing a mono aza-15 - crown-5 and a DMA receptor is synthesized , see Scheme 1 . - pubmed
Therefore , these results indicate that glutathione degradation occurs within vacuoles or at the tonoplast , rather than at the plasmalemma or in the apoplast . - pubmed
Activity of PXD101 , a histone deacetylase inhibitor , in preclinical ovarian cancer studies . - pubmed
In the current investigation , we examined the activity of PXD101 , a potent histone deacetylase inhibitor , used alone or in combination with clinically relevant chemotherapeutics ( docetaxel , paclitaxel , and carboplatin ) , in preclinical in vitro and in vivo models of ovarian cancer . - pubmed
In support of these findings , PXD101 was shown to increase the acetylation of alpha - tubulin induced by docetaxel and the phosphorylation of H2AX induced by carboplatin . - pubmed
AIM : The aim of this study was to examine the potential effects of COCs on women with hypoactive sexual desire disorder ( HSDD ) . - pubmed
A two - tailed t - test comparison was made between the two groups comparing free and total testosterone and sex hormone - binding globulin ( SHBG ) . - pubmed
MAIN OUTCOME MEASURES : The main outcome measures are the differences between the two groups comparing free testosterone , total testosterone , and SHBG . - pubmed
The SHBG was significantly higher in the group on COCs compared with those who were not on COCs . - pubmed
Production of 1,25 ( OH )( 2 ) D ( 3 ) is catalysed by the enzyme 25 - hydroxyvitamin D ( 3 )- 1alpha - hydroxylase ( CYP27B1 ) . - pubmed
Cells were treated with either parathyroid hormone ( PTH ) , 1,25 ( OH )( 2 ) D ( 3 ) , transforming growth factor - beta ( TGF - beta ) or insulin - like growth factor-1 ( IGF-1 ) and luciferase activity was measured 24h later . - pubmed
The results showed that 1,25 ( OH )( 2 ) D ( 3 ) did not alter expression of the reporter construct , however treatment with PTH , IGF-1 and TGF - beta decreased expression by 18 , 53 and 58 % respectively . - pubmed
The incretin effect was measured as the difference in insulin secretion ( area under the curve [ AUC ] ) in response to an oral glucose tolerance test ( OGTT ) and to an isoglycemic intravenous glucose test . - pubmed
One month after RY - GBP , body weight decreased by 9.2 + /- 7.0 kg , oral glucose - stimulated GLP-1 ( AUC ) and GIP peak levels increased significantly by 24.3 + /- 7.9 pmol x l(-1 ) x min(-1 ) ( P & lt ; 0.0001 ) and 131 + /- 85 pg / ml ( P = 0.007 ) , respectively . - pubmed
Gliadin fibers have high strength ( 120 MPa ) and elongation ( 25 % ) compared to similar collagen fibers that were crosslinked with glutaraldehyde ( strength of about 44 MPa and elongation of 14 % ) . - pubmed
18FDG - PET scan and T1 enhanced MRI follow - up studies were performed between 3 and 5 months after treatment . - pubmed
Intracellular pH homeostasis and serotonin - induced pH changes in Calliphora salivary glands : the contribution of V - ATPase and carbonic anhydrase . - pubmed
Blowfly salivary gland cells have a vacuolar - type H (+)- ATPase ( V - ATPase ) in their apical membrane that energizes secretion of a KCl - rich saliva upon stimulation with serotonin ( 5 - hydroxytryptamine , 5 - HT ) . - pubmed
We have used BCECF to study microfluometrically whether V - ATPase and carbonic anhydrase ( CA ) are involved in intracellular pH ( pH(i ) ) regulation , and we have localized CA activity by histochemistry . - pubmed
We show : ( 1 ) mean pH(i ) in salivary gland cells is 7.5+/-0.3 pH units ( N=96 ) , higher than that expected from passive H (+ ) distribution ; ( 2 ) low 5 - HT concentrations ( 0.3 - 3 nmol l(-1 ) ) induce a dose - dependent acidification of up to 0.2 pH units , with 5 - HT concentrations & gt;10 nmol l(-1 ) , causing monophasic or multiphasic pH changes ; ( 3 ) the acidifying effect of 5 - HT is mimicked by bath application of cAMP , forskolin or IBMX ; ( 4 ) salivary gland cells exhibit CA activity ; ( 5 ) CA inhibition with acetazolamide and V - ATPase inhibition with concanamycin A lead to a slow acidification of steady - state pH(i ) ; ( 6 ) 5 - HT stimuli in the presence of acetazolamide induce an alkalinization that can be decreased by simultaneous application of the V - ATPase inhibitor concanamycin A ; ( 7 ) concanamycin A removes alkali - going components from multiphasic 5 - HT - induced pH changes ; ( 8) NHE activity and a Cl (-)-dependent process are involved in generating 5 - HT - induced pH changes ; ( 9 ) the salivary glands probably contain a Na (+)- driven amino acid transporter . - pubmed
We conclude that V - ATPase and CA contribute to steady - state pH(i ) regulation and 5 - HT - induced outward H (+ ) pumping does not cause an alkalinization of pH(i ) because of cytosolic H (+ ) accumulation attributable to stimulated cellular respiration and AE activity , masking the alkalizing effect of V - ATPase - mediated acid extrusion . - pubmed
Arginase I , an abundant manganese ( Mn)-binding protein in the liver , requires Mn as an essential element to exhibit maximal enzyme activity . - pubmed
In the present study , we examined the effect of MeHg on hepatic arginase I in vivo and in vitro . - pubmed
With purified arginase I , we found that interaction of MeHg with arginase I caused the aggregation of arginase I as evaluated by centrifugation and subsequent precipitation , and then the reduction of catalytic activity . - pubmed
Experiments with organomercury column confirmed that arginase I has reactive thiols that are covalently bound to organomercury . - pubmed
While MeHg inhibited arginase I activity , Mn ions were released from this enzyme . - pubmed
These results suggest that MeHg - mediated suppression of hepatic arginase I activity in vivo is , at least in part , attributable to covalent modification of MeHg or substantial leakage of Mn ions from the active site . - pubmed
Chondroitin sulfate ( CS ) dermatan sulfate ( DS ) , and CS / DS hybrid chains are biologically active like heparan sulfate , and structurally the most complex species of the glycosaminoglycan family along with heparan sulfate . - pubmed
They function as regulators of functional proteins such as growth factors , cytokines , chemokines , adhesion molecules , and lipoproteins through interactions with the ligands of these proteins via specific saccharide domains . - pubmed
Recent microsequencing of CS / DS oligosaccharides that bind growth factors , such as pleiotrophin , and various monoclonal antibodies against CS / DS , have revealed a considerable number of unique oligosaccharide sequences . - pubmed
MATERIALS & amp ; METHODS : Two antiprostate - specific antigen scFvs mutants -- one with an 11 - residue c - myc ( referred as scFvB80 - M1 ) and the other with a lysine - enriched His 6 - tagging peptide attached to their C - termini ( referred as scFvB80 - M2)--were prepared . - pubmed
These two scFv mutants were conjugated directly with CdSe / ZnS Qdots and their binding activities were measured and compared . - pubmed
RESULTS & amp ; DISCUSSION : Both scFv mutants can be conjugated covalently with CdSe / ZnS Qdots ; however , the resulting conjugates exhibit significantly different affinities in the prostate - specific antigen fluoroimmunoassays -- the binding activity of scFvB80 - M2 / Qdots is equivalent of that of free scFvB80 and four times of that of scFvB80 - M1 / Qdots . - pubmed
Upon strand separation by a helicase and ATP , the beacon strand forms an intramolecular hairpin that brings the tethered fluorescent and quencher molecules into juxtaposition , quenching fluorescence . - pubmed
Calcium binding of rE - Syt2 triggered structural rearrangements and initiated reversible multimerization of the protein in vitro . - pubmed
IV mannitol ) , the appropriate treatment for CFP remains unclear to many physicians . - pubmed
It is proposed to study the effect of ALA on TD induced by HAL and to correlate it with oxidative stress by studying total antioxidant status and lipid peroxidation ( LP ) . - pubmed
ALA was suspended in 0.2 % carboxy methyl cellulose at a dose of 25 , 50 and 100 mg / kg and was administered orally by oral gavage 1 h before HAL on 21st day of treatment . - pubmed
Fitting a single - phase model to the post - exercise changes in heart rate and oxygen uptake . - pubmed
Half - time for VO2 negatively correlated with square root of exercise duration ( within - subject r = -0.629 , p & lt ; 0.001 ) , the total post - exercise oxygen uptake till t(1/2 ) was thus constant irrespectively of exercise intensity . - pubmed
The renal distal convoluted tubule ( DCT ) has an essential role in maintaining systemic magnesium ( Mg ( 2 + ) ) concentration . - pubmed
The DCT is the final determinant of plasma Mg ( 2 + ) levels , as the more distal nephron segments are largely impermeable to Mg ( 2 + ) . - pubmed
A large fraction of this resides in the DCT , namely , ( i ) the transient receptor potential channel melastatin subtype 6 ( TRPM6 ) , a divalent cation - permeable channel located at the luminal membrane of the DCT , facilitates Mg ( 2 + ) entry from the pro - urine into the cell ; ( ii ) the epidermal growth factor is a novel hormone regulating active Mg ( 2 + ) transport through TRPM6 ; ( iii ) the voltage - gated K (+ ) channel , Kv1.1 , establishes a favorable luminal membrane potential for TRPM6 - mediated Mg ( 2 + ) transport ; ( iv ) the Na (+)/ K (+)- ATPase gamma - subunit ( gamma - Na (+)/ K (+)- ATPase ) was identified as mutated protein in a family with isolated dominant hypomagnesemia . - pubmed
The aim of the present in vitro investigation was to elucidate the impact of AB0 blood group - dependent vWF concentrations on eptifibatide and abciximab mediated inhibition of GPIIb / IIIa function . - pubmed
If measured with the collagen / ADP cartridge , blood group 0 was associated with a prolonged mean baseline closure time in comparison with blood group A ( 94.3 + /- 14.6 s vs. 74.6 + /- 9.9 s , p = 0.007 ) which was paralleled by reduced concentrations of vWF and factor VIII . - pubmed
The effects of triphosphopyridine nucleotide and of adenosine triphosphate on pigeon liver oxalacetic carboxylase . - pubmed
Addition of induction therapy offsets the hazard of marked calcineurin minimization among renal transplant recipients treated with sirolimus . - pubmed
Association between FokI polymorphism in vitamin D receptor gene and susceptibility to spinal tuberculosis in Chinese Han population . - pubmed
BACKGROUND AND AIMS : Studies have shown that the vitamin D receptor ( VDR ) gene polymorphisms may be associated with pulmonary tuberculosis ( TB ) and play important roles in the regulation of calcium in a variety of tissues including bone . - pubmed
Adenylosuccinate lyase ( ADSL ) is a bifunctional enzyme acting in de novo purine synthesis and purine nucleotide recycling . - pubmed
Nucleotide sequence analysis of the PCR amplicons followed by GenBank nucleotide BLASTn analysis revealed five novel DNA sequences ; translated amino acid BLASTx analysis confirmed sequence similarity to class 1b major structural proteins encoded by csnA , fotA , and fasA. - pubmed
Accuracy of parathyroid imaging : a comparison of planar scintigraphy , SPECT , SPECT - CT , and C-11 methionine PET for the detection of parathyroid adenomas and glandular hyperplasia . - pubmed
PURPOSE : To compare the accuracy of planar scintigraphy , single photon emission computed tomography ( SPECT ) , SPECT - CT , and positron emission tomography ( PET ) with C-11 methionine for the pre - operative detection of parathyroid adenomas . - pubmed
In the case of negative scintigraphic findings and proven hyperparathyroidism , additional C-11 methionine PET or PET - CT is recommended . - pubmed
The scope of this study is to modify a Surface Plasmon Resonance ( SPR ) sensor slide with isophthalic acid to evaluate the possible application on the detection of copper ( II ) ions in aqueous media by total internal reflection ellipsometry . - pubmed
Isophthalic acid monolayer modified gold slides were used for in situ detection of aqueous Cu ( 2 + ) solution with the SPR enhanced total internal reflection ellipsometry ( SPRe - TIRE ) technique . - pubmed
Gibberellins negatively regulate low temperature - induced anthocyanin accumulation in a HY5 / HYH - dependent manner . - pubmed
Recently , two bZIP transcription factors LONG HYPOCOTYL 5 ( HY5 ) and HOMOLOG OF HY5 ( HYH ) were identified to play an important role in the process of low temperature - induced anthocyanin accumulation . - pubmed
However , the mechanism by which HY5 / HYH regulates anthocyanin accumulation under low temperature still remains unclear . - pubmed
Moreover , hy5hyh mutant was insensitive to PAC in enhancing anthocyanin accumulation under low temperature . - pubmed
From these data we propose that , together with HY5 / HYH , GA signaling may play an important role during low temperature - induced anthocyanin accumulation . - pubmed
At least two pesticides were detected at every site including insecticides , fungicides , herbicides , and the Reef Plan 's ( 2009 ) five priority photosystem II ( PSII ) herbicides ( diuron , atrazine , hexazinone , tebuthiuron and ametryn ) . - pubmed
On morphology and strain field of Ge / Si(001 ) Islands according to TEM phase imaging method . - pubmed
Cd treatments led to a decline in mycorrhizal infection ( MI ) , nodule number and dry weights which was accompanied by reductions in leghemoglobin content , nitrogenase activity , organic acid contents . - pubmed
The lard - rich diet increased liver CL synthase gene expression while the fish oil - rich diet increased the ( n-3 ) polyunsaturated fatty acids content in CL . - pubmed
These include , amongst others , the Rho GTPase , focal adhesion kinase , cyclooxygenase-2 , beta - catenin signalling and calcium signalling . - pubmed
A total of 164 bacteria - specific probes were designed from 16S rRNA gene sequences to target 12 bacteria species , including lactic acid bacteria and selected food pathogens . - pubmed
In the present study , we administered CO to the same model of ALI only after resuscitation and examined whether it exerted a therapeutic effect without adverse events on HSR - induced ALI , since treatment of animals with CO prior to HSR did not prevent lung injury . - pubmed
This was determined from an observed increase in the number of cells positive for transferase - mediated dUTP - fluorescein isothiocyanate ( FITC ) , nick - end labeling staining and activated caspase-3 . - pubmed
The protective effects of CO against lung injury were notably associated with an increase in the protein expression level of peroxisome proliferator - activated receptor ( PPAR )- gamma , an anti - inflammatory transcriptional regulator in the lung . - pubmed
Moreover , CO inhalation did not affect the hemodynamic status or tissue oxygenation during HSR . - pubmed
These findings suggest that inhalation of CO at a low concentration exerts a potent therapeutic effect against HSR - induced ALI and attenuates the inflammatory cascade by increasing PPAR - gamma protein expression . - pubmed
The affinity tag consists of an N - terminal 6xHis - tag , a choline binding domain followed by a proteolytic site specific for the TEV ( tobacco etch virus ) endopeptidase . - pubmed
Based on the choline - binding His - tag combination the new 16.5 kDa tag was named CHiC. - pubmed
The STAT5 Inhibitor Pimozide Displays Efficacy in Models of Acute Myelogenous Leukemia Driven by FLT3 Mutations . - pubmed
We have identified the psychotropic drug pimozide as an effective inhibitor of STAT5 function . - pubmed
Pimozide inhibits the tyrosine phosphorylation of STAT5 , leading to the death of AML cells through the induction of apoptosis . - pubmed
Pimozide shows a combinatorial effect with the tyrosine kinase inhibitors midostaurin ( PKC412 ) and sunitinib in the inhibition of STAT5 tyrosine phosphorylation and the induction of apoptosis . - pubmed
The HSP90 inhibitor NVP - AUY922 - AG inhibits the PI3 K and IKK signalling pathways and synergizes with cytarabine in acute myeloid leukaemia cells . - pubmed
Here , we investigated HSP90 as a molecular target for acute myeloid leukaemia ( AML ) using the novel HSP90 inhibitor NVP - AUY922 - AG . - pubmed
NVP - AUY922 - AG induced increases in HSP70 expression and depletion of total AKT , IKKalpha and IKKbeta in cell lines and primary blasts . - pubmed
This study shows that the novel HSP90 inhibitor NVP - AUY922 - AG has significant single agent activity in AML cells and is synergistic with Ara - C. - pubmed
A sensitive analytical method was developed for the simultaneous determination of glyphosate , glufosinate and aminomethylphosphonic acid by capillary electrophoresis with laser - induced fluorescence detection ( CE - LIF ) . - pubmed
Every quarter , mean levels and prevalence of conformity were collected for the following indicators : anemia , dialysis dose , serum calcium and phosphorus , PTH , 25OH - vitamin D , albumin , serum bicarbonate , LDL - cholesterol , serum beta2 - microglobulin , systolic and diastolic blood pressure , intradialytic hypotension and vascular access . - pubmed
An improvement in the percentage of conformity was observed for hemoglobin , dialysis dose , phosphates , PTH , serum bicarbonate and beta2 - microglobulin in the global population . - pubmed
Finally , we suggest that the definition and targets of some indicators , especially hypertension and LDL - cholesterol , be reviewed , since evidence of their association with mortality is not demonstrated . - pubmed
Reactions at room temperature in water - ethanol mixtures give rise to hydrated kinetic products , including two new crystal structures , [ Li2 ( D , L - tartrate )( H2O ) 2 ] n in P1 and [ Li2 ( meso - tartrate )( H2O )( 0.5 )] n in C222(1 ) , whose structures are contrasted with known anhydrous products . - pubmed
METHODS : Planar and SPECT / CT scans of a water phantom containing a 70 ml bottle filled with lutetium-177 ( Lu ) were acquired over 60 days . - pubmed
RESULTS : For count rates that were about two - fold higher than typically seen in post - therapy Lu scans , the maximum DTCF reached a level of about 17 % . - pubmed
The properties of mutants affecting the biosynthesis of leucine and its regulation suggest that the expression of LEU1 and LEU2 ( structural genes encoding isopropylmalate isomerase and beta - isopropylmalate dehydrogenase , respectively ) is controlled by a complex of a - isopropylmalate and a regulatory element ( the LEU3 gene product ) . - pubmed
Initial investigations revealed atrial fibrillation ( AF ) and pericardial effusion , further investigations unraveled primary hypothyroidism ( thyroid stimulating hormone ) of 34.7 muIU / ml and total thyroxine ( T4 ) of 5.57 mug / dl ) . - pubmed
The incidence of four - year MACE was significantly higher in BA ( 75.2 % ) than in DES implantation ( 45.8 % , p<0.001 ) , mainly because of the lower TLR rate in DES implantation ( 60.5 % vs. 27.6 % , p<0.001 ) . - pubmed
Radiosynthesis of carbon-11 and fluorine-18 labelled radiotracers to image the ionotropic and metabotropic glutamate receptors . - pubmed
l - Glutamate is the major neurotransmitter in the central nervous system and activates both ionotropic and metabotropic receptors . - pubmed
A tetradecapeptide somatostatin dicarba - analog : Synthesis , structural impact and biological activity . - pubmed
Remarkably , this alkene - bridged somatostatin analog is highly selective against somatostatin receptors 1 and 5 , suggesting that conformational rigidity is not required for the efficient interaction of somatostatin analogues with these two receptors . - pubmed
Washed leaves were crushed in a meat - mill in an isotonic or slightly hypertonic medium containing sucrose , EDTA , MgSO4 , bovine serum albumin , mercaptoethanol , KH2PO4 , and HEPES as a buffer substance . - pubmed
After resuspension in a washing medium , the pellet was centrifuged in a swing - out rotor for 30 min at 35000xg on a density gradient generated from a silica sol ( " Ludox HS 40 " ) . - pubmed
The respiration rate was 29.5 muatoms O / mg protein - N / h with alpha - ketoglutarate as a substrate . - pubmed
The arsenic ambient water quality criteria ( AWQC ) , based on the assumption of a linear mode of action for skin cancer risk , results in an allowable limit of 0.018ppb in ambient waters ; the drinking water Maximum Contaminant Level ( MCL ) was determined using a similar linear approach . - pubmed
tuberculosis sigma ( K ) in complex with the cytosolic domain of RskA ( RskAcyto ) revealed a disulfide bridge in the -35 promoter - interaction region of sigma ( K ) . - pubmed
Biochemical experiments reveal that the redox potential of the disulfide - forming cysteines in sigma ( K ) is consistent with its role as a sensor . - pubmed
The disulfide bond in sigma ( K ) influences the stability of the sigma ( K )- RskAcyto complex but does not interfere with sigma ( K )-promoter DNA interactions . - pubmed
Photoluminescence ( PL ) measurements of ZnO : Eu3 + showed a strong red luminescence emission . - pubmed
The intensity of Eu3 + PL decreased with the increasing Eu3 + concentration and the concentration quenching mechanism was presented based on non - radiative recombination processes in ZnO and the energy back - transfer from the excited state of Eu3 + to the ZnO host . - pubmed
Association between single nucleotide polymorphisms of asporin ( ASPN ) and BMP5 with the risk of knee osteoarthritis in a Chinese Han population . - pubmed
Fe3O4 peroxidase mimetics as a general strategy for the fluorescent detection of H2O2 - involved systems . - pubmed
We developed a general H2O2 - involved fluorescence system using Fe3O4 magnetic microspheres as peroxidase mimetics and benzoic acid ( BA ) as indicator . - pubmed
Glucose oxidase hydrolyzes glucose , in the presence of oxygen , to H2O2 followed by the activation of Fe3O4 MMs , resulting in the catalyzed oxidation of benzoic acid . - pubmed
Reducing AgNO3 with NaBH4 in CNF suspensions produced smaller but more uniform Ag nanospheres ( AgNSs ) with increasing Ag(+)/CNF ratios . - pubmed
CNF bound AgNSs were facilely transformed to AgNPs by etching with H2O2 , supporting the capping and shape - regulating capability of CNFs . - pubmed
The AgNPs transformed from CNF bound AgNSs are similar to those synthesized directly , but more stable against H2O2 . - pubmed
The CNF surface bound AgNPs were easily fabricated into freestanding CNF / AgNPs films that showed excellent surface enhanced Raman scattering of Rhodamine 6 G with analytical enhancement factor of 5 x 10(3 ) in contrast to none from the CNF / AgNSs film . - pubmed
Tetraspanin CD9 is considered to be a serious candidate molecule participating in these events . - pubmed
Since caffeine is a mixed nonspecific A ( 1 ) and A ( 2A ) adenosine receptor antagonist , we measured also the influence of antagonists and agonists of these receptors on SWD expression . - pubmed
GAERS were equipped with four cortical electrodes over the frontoparietal cortex and the cumulated duration and number of SWDs were recorded for 120 min after the injection of increasing doses of caffeine , specific antagonists and agonists of A ( 1 ) and A ( 2A ) adenosine receptors . - pubmed
These data show that the acute exposure to low doses of caffeine , or A(1 ) and A ( 2A ) adenosine receptor antagonists reduces SWD expression in GAERS , independently from any effect on motor activity . - pubmed
Two extrinsic fluorescent probes , 3 -( dimethylamino )- 8,9,10,11 - tetrahydro-7H - cyclohepta [ a ] naphthalen-7 - one ( 1 ) and 7 -( dimethylamino )- 2,3 - dihydrophenanthren-4 ( 1H )- one ( 2 ) , are used to probe the unfolding of human serum albumin by sodium dodecyl sulfate ( SDS ) . - pubmed
Each subsequent equivalent of SDS gives rise to a dramatic increase in polarity until HSA is saturated with seven molecules of SDS at the end of the specific binding domain . - pubmed
This result is consistent with greater exposure of the carbonyl group in 2 , but not the bulk of 2 , to the aqueous solvent in site II of the SDS - saturated HSA complex . - pubmed
To validate these observations , the expression of Keap1 protein was restored in arsenite - transformed cells by treatment with a DNA methyltransferase inhibitor , 5 - aza-2 '- deoxycytidine ( 5 - Aza - dC ) , and protein levels of Nrf2 and colony formation were then determined after these treatments . - pubmed
Results showed that enhancement of Keap1 expression by 5 - Aza - dC significantly reduced Nrf2 and its target antioxidant enzyme levels , and that in turn suppressed cell proliferation and colony formation of the transformed cells . - pubmed
The inhibition of noradrenaline re - uptake by propiverine mainly occurs at the urethral level and partially in the central nervous system , and might stimulate the smooth muscle of the bladder neck and proximal urethra through alpha1 - adrenergic receptors , as well as stimulating the striated muscle of the urethra and pelvic floor by activation of spinal motoneurons . - pubmed
The in - vitro antioxidant effect of ECP was investigated using 1 - diphenyl-2 - picrylhydrazyl , reducing power , beta - carotene bleaching and thiobarbituric acid reactive substances assays . - pubmed
BACKGROUND / OBJECTIVE : The mechanistic target of rapamycin ( mTOR ) is a serine - threonine kinase that functions into distinct protein complexes ( mTORC1 and mTORC2 ) that regulate energy homeostasis . - pubmed
RESULTS : We first report that systemic ( brain and periphery ) overexpression of DEPTOR prevents high - fat diet - induced obesity , improves glucose metabolism and protects against hepatic steatosis . - pubmed
Ten - eleven translocation enzyme 2 ( TET2 ) protein , one member of TET family , could rely on alpha - ketoglutarate ( alpha - KG ) as cosubstrate to exhibit catalytic activity of DNA demethylation . - pubmed
Further analysis showed that alpha - KG concentration had a positive correlation with both haemoglobin A1c ( HbA1C ) and 2 h postprandial blood glucose ( PBG ) ( r = 0 393 , P = 0 005 ; r = 0 320 , P = 0 025 , respectively ) . - pubmed
BACKGROUND : Aerobic interval training ( IT ) seems to be superior to continuous training ( CT ) in improving exercise capacity ( peak oxygen uptake ( VO2 ) ) in coronary artery disease ( CAD ) patients in some , but not in all studies . - pubmed
Higher lactate levels were reached after IT sessions ( ITw 5.42 + - 1.42 mmol / l , ITc 5.05 + - 1.38 mmol / l ) compared to CT sessions ( CTw 2.45 + - 1.04 mmol / l , CTc 3.41 + - 1.44 mmol / l ) ( p & lt ; 0.01 ) . - pubmed
Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide - rich hot spring . - pubmed
Alkaline sulfide - rich hot springs provide a unique environment for microbial community and arsenic ( As ) biogeochemistry . - pubmed
In this study , a representative alkaline sulfide - rich hot spring , Zimeiquan in the Tengchong geothermal area , was chosen to study arsenic geochemistry and microbial community using Illumina MiSeq sequencing . - pubmed
High ratios of As ( V )/ AsSum ( total combined arsenate and arsenite concentrations ) ( 0.59 - 0.78 ) , coupled with high sulfide ( up to 5.87 mg / L ) , were present in the hot spring 's pools , which suggested As ( III ) oxidation occurred . - pubmed
This study improves our understanding of microbially - mediated As transformation in alkaline sulfide - rich hot springs . - pubmed
In this study , we used glioma cell lines and H2O2 to investigate the role of JNK in glioma cell parthanatos induced by oxidative stress . - pubmed
We found that exposure to H2O2 not only induced intracellular accumulation of ROS but also resulted in glioma cell death in a concentration- and incubation time - dependent manner , which was accompanied with cytoplasmic formation of PAR polymer , expressional upregulation of PARP-1 , mitochondrial depolarization , and AIF translocation to nucleus . - pubmed
Moreover , the phosphorylated level of JNK increased markedly with the extension of H2O2 exposure time . - pubmed
Either attenuation of intracellular ROS with antioxidant NAC or inhibition of JNK phosphorylation with SP600125 or JNK siRNA could significantly prevent H2O2 - induced parthanatos in glioma cells . - pubmed
Additionally , inhibition of JNK with SP600125 alleviated intracellular accumulation of ROS and attenuated mitochondrial generation of superoxide . - pubmed
Light - induced vasopressin release enhances the responses of SCN neurons to light . - pubmed
Many SCN neurons express vasopressin , and it has been assumed that the role of vasopressin in the SCN reflects the activity of these cells . - pubmed
Here we show that vasopressin is also expressed in many retinal cells that project to the SCN . - pubmed
Light - evoked vasopressin release contributes to the responses of SCN neurons to light , and enhances expression of the immediate early gene c - fos in the SCN , which is involved in photic entrainment of circadian rhythms . - pubmed
The proposed model is applied to two motivating studies of amyotrophic lateral sclerosis : a clinical trial of ceftriaxone and the Pooled Resource Open - Access ALS Clinical Trials database . - pubmed
MATERIAL AND METHODS : Type 4 commercially pure titanium disks and 5 porcelain dentin shades ( A2 , A3 , B2 , C2 , and D3 ) were used . - pubmed
CONCLUSIONS : The minimum ceramic thickness required to mask the underlying titanium color is 0.5 mm for all shades except D3 , which only needs 0.4 mm . - pubmed
Astragaloside IV protects against podocyte injury via SERCA2 - dependent ER stress reduction and AMPKalpha - regulated autophagy induction in streptozotocin - induced diabetic nephropathy . - pubmed
Docosahexaenoic acid enrichment in NAFLD is associated with improvements in hepatic metabolism and hepatic insulin sensitivity : a pilot study . - pubmed
Bicarbonate Recycling by HIF-1 - Dependent Carbonic Anhydrase Isoforms 9 and 12 Is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells . - pubmed
We tested the hypothesis that a network comprising HIF-1alpha , carbonic anhydrase ( CA ) 9 and 12 isoforms , and sodium - coupled bicarbonate cotransporters ( NBCs ) buffer intracellular pH through coordinated bicarbonate recycling . - pubmed
Contrary to the current understanding of NP cell metabolism , analysis of metabolic - flux data from Seahorse XF analyzer showed that CO2 hydration contributes a significant source of extracellular proton production in NP cells , with a smaller input from glycolysis . - pubmed
Because enzymatic hydration of CO2 is catalyzed by plasma membrane - associated CAs we measured their expression and function in NP tissue . - pubmed
As expected , inhibition of CA activity decreased extracellular acidification rate independent of changes in HIF activity or lactate / H+ efflux . - pubmed
These results suggested that extracellular bicarbonate generated by CA9 / 12 is recycled to buffer cytosolic pH fluctuations . - pubmed
Taken together , our studies show for the first time that bicarbonate buffering through the HIF-1alpha - CA axis is critical for NP cell survival in the hypoxic niche of the intervertebral disc . - pubmed
Simulations using PK / PD models showed that CYP2C9 genotype is the factor that affects the PDs of meloxicam . - pubmed
Curcumin suppresses MUC5AC production via interfering with the EGFR signaling pathway . - pubmed
Curcumin was able to counteract the endothelial growth factor ( EGF)-stimulated mRNA and protein expression of MUC5AC . - pubmed
In addition , curcumin treatment prevented EGF - induced phosphorylation of EGF receptor ( EGFR ) as well as the downstream AKT and signal transducer and activator of transcription 3 ( STAT3 ) , while inhibition of PI3 K and STAT3 signaling significantly attenuated the expression of MUC5AC that was induced by EGF . - pubmed
Furthermore , EGF - induced increases in the levels of phosphorylated STAT3 in the nuclear fraction were inhibited by curcumin and PI3 K inhibitors . - pubmed
In addition , treatment with curcumin significantly decreased MUC5AC and EGFR expression in a time - dependent manner under basal conditions . - pubmed
Bayesian statistics identified 12 metabolites associated with clinical characteristics of postprandial vascular endothelial function , such as flow - mediated dilation ( FMD ) , postprandial plasma markers of oxidative stress and NO status . - pubmed
Serum blood urea nitrogen , creatinine , and urinary and serum kidney injury molecule-1 KIM-1 levels were measured . - pubmed
Hemoglobin ( Hb ) target levels can be achieved through more frequent intravenous ( IV ) iron use with lower ESA dose , or with less iron dosing but higher ESA . - pubmed
ESA therapy to correct anemia may result in severe arterial and venous thrombotic complications and the evidence base evaluating hard clinical outcomes related to the use of IV iron is sparse . - pubmed
In Poland the use and dose of IV iron was 35 - 72 % higher than that in Portugal ( p & lt ; 0.001 ) during three consecutive months ; use and dose of ESA was 61 % higher in Portugal ( 5034 vs 3133 IU ( adjusted)/week , p & lt ; 0.001 ) . - pubmed
CONCLUSIONS : Administration of high doses of IV iron in routine clinical HD practice may not be associated with considerable harm . - pubmed
Correction to : Unexpected Benefit from Alpelisib and Fulvestrant in a Woman with Highly Pre - treated ER - Positive , HER2 - Negative PIK3CA Mutant Metastatic Breast Cancer . - pubmed
Silver Nanoparticle - Induced Phosphorylation of Histone H3 at Serine 10 Involves MAPK Pathways . - pubmed
The phosphorylation of histone H3 at serine 10 ( p - H3S10 ) has been shown to be closely correlated with mitotic chromosome condensation . - pubmed
We previously reported that intracellular silver nanoparticles ( AgNPs ) release Ag ions that alter actin filament dynamics , leading to the activation of Aurora kinases and the formation of p - H3S10 through a mechanism clearly different from that occurring during mitosis . - pubmed
We observed different effects of AgNO3 ( Ag+ ) and AgNPs on cells , and only the JNK inhibitor suppressed the temporary AgNO3 - induced formation of p - H3S10 . - pubmed
The photochemistry of anthracene , a new class of photoresist for direct laser writing , is used to enable visible - light - gated control over the mechanical properties of 3D microstructures post - manufacturing . - pubmed
Applying correct WST class prediction from RFA modelling , new reference intervals were calculated for activin B and associated pathology markers , where 24 - h urinary creatinine clearance , serum urea and serum activin B showed the best potential as diagnostic markers . - pubmed
HuR regulates phospholamban expression in isoproterenol - induced cardiac remodeling . - pubmed
OBJECTIVE : The elevated expression of phospholamban ( PLB ) has been observed in heart failure and cardiac remodeling , inhibiting the affinity of Ca2 + pump to Ca2 + thereby impairing heart relaxation . - pubmed
However , deletion of HuR in cardiomyocytes mitigated the effect of ISO in inducing PLB expression and reducing beta1 - AR expression , in turn aggravating ISO - induced myocardial hypertrophy and cardiac fibrosis . - pubmed
The HuR - PLB and HuR - beta1 - AR regulatory processes impact on ISO - induced cardiac remodeling . - pubmed
TRANSLATIONAL PERSPECTIVE : PLB , the reversible inhibitor of SERCA2A , is of critical importance for the SR Ca2 + sequestration thereby the excitation - contraction coupling of myocytes and myocardial remodeling . - pubmed
The activity of PLB could be inhibited by beta1 - AR - cAMP - PKA pathway through PKA - mediated protein phosphorylation . - pubmed
The escape latency ( EL ) ; cerebral infarction area ratio ; positive apoptosis ; neuron pathological changes ; auto - phagosome numbers ; inflammatory factor contents ; mRNA and protein expressions of Beclin-1 , Caspase-3 and LC3II / I ; and the phosphorylation level of JNK were decreased , while the times across platform and the times stayed in the quadrant of the original platform were increased after dexmedetomidine treatment . - pubmed
Decreased H3K9 acetylation level of LXRalpha mediated dexamethasone - induced placental cholesterol transport dysfunction . - pubmed
Results showed that dexamethasone increased maternal serum total cholesterol ( TC ) , high - density lipoprotein - cholesterol ( HDL - C ) , low - density lipoprotein - cholesterol ( LDL - C ) levels , as well as placental cholesterol synthesis and TC concentration , while reduced fetal birth weight , and serum TC , HDL - C and LDL - C levels . - pubmed
Meanwhile , the expression of placental cholesterol transporters , including low - density lipoprotein receptor ( LDLR ) , scavenger receptor class B type I ( SR - B1 ) and ATP - binding cassette transporter A1 and G1 ( ABCA1 and ABCG1 ) were decreased by dexamethasone . - pubmed
Dexamethasone ( 2500 nM ) induced GR translocation into nucleus and recruited HDAC3 . - pubmed
Furthermore , LXRalpha agonist and GR inhibitor reversed respectively dexamethasone - induced the expression inhibitions of cholesterol transporter and LXRalpha , and HDAC3 siRNA reversed the H3K9ac level of LXRalpha promoter and its expression . - pubmed
Together , dexamethasone impaired placental cholesterol transport and eventually decreased fetal cholesterol levels , which is related to the down - regulation of LXRalpha mediated by GR / HDAC3 / H3K9ac signaling . - pubmed
Biochemical assays of UGT76C family revealed that UGT76C5 ( encoded by At5g05890 , previously identified as NaNGT ) was a multiple functional nicotinate N - glycosyltransferase , with high preference to UDP - xylose and UDP - arabinose . - pubmed
We dissect the following three key molecular events that occur as Rad4 / XPC tries to recognize and bind to DNA lesions / mismatches : ( a ) the association of Rad4 / XPC with the damaged / mismatched DNA , ( b ) the insertion of a lesion - sensing beta - hairpin of Rad4 / XPC into the damage / mismatch site and ( c ) the flipping of a pair of nucleotide bases at the damage / mismatch site . - pubmed
LC - MS / MS revealed that PDD is associated with the 5 - methylaminomethyl-2 - thiouridine modification of chloroplast tRNA . - pubmed
Because of the many correlations between oxytocin binding and bioactivity , it appears that oxytocin binding sites on the plasma membrane of target cells constitute the recognition part of oxytocin receptors . - pubmed
The effects lasted for 24 h. Intraamniotic injection of 10 mg oPRL prevented or reversed a doubling of water and electrolyte content of the fetal extracellular fluid ( ECF ) volume in the face of hypertonic amniotic fluid . - pubmed
Radioactively - labeled neurophysin bound to polymethylmethacrylic particles was similarly infected into the animals . - pubmed
Immunoperoxidase histochemical techniques were applied to formalin - fixed kidney slices and demonstrated the presence of neurophysin - like material localized in the cells of proximal tubules of the cortex and medulla . - pubmed
The reference nootropic drug piracetam given in a dose 200 mg / g for 9 days after surgery promoted CAAR recovery . - pubmed
Another peptide analogue of piracetam -- pyroglutamyl - asparagine amide ( II ) showed no promoting effect on CAAR recovery after frontal lobectomy . - pubmed
Reaction of dioxygen with cytochrome c oxidase reduced to different degrees : indications of a transient dioxygen complex with copper - B. - pubmed
The reactions of the fully reduced , three - electron - reduced , and mixed - valence cytochrome oxidase with molecular oxygen have been followed in flow - flash experiments , starting from the CO complexes , at 445 and 830 nm at pH 7.4 and 25 degrees C. With the fully reduced and the three - electron - reduced enzyme , four kinetic phases with rate constants in the range from 1 x 10(5 ) to 10(3 ) s-1 can be observed . - pubmed
It is suggested that O2 initially binds to reduced CuB and is then transferred to cytochrome a3 before electron transfer from cytochrome a / CuA takes place . - pubmed
RESULTS : Training significantly increased maximal ventilatory oxygen consumption ( VO2MAX ) , and there was a decrease in ambulatory diastolic blood pressure ( DBP ) which did not reach statistical significance . - pubmed
Atenolol alone significantly reduced ambulatory systolic blood pressure ( SBP ) and DBP . - pubmed
This chemical modification increases the dissociation constant of the apoflavodoxin - flavin mononucleotide ( FMN ) complex from 0.4 nM to 2 microM. - pubmed
The signals at 109.4 ppm and 112.2 ppm due to the cyanylated apoflavodoxin were unstable at 28 degrees C , and they were slowly replaced signals at 114.5 ppm and 115.3 ppm which are attributed to an inactive form of the apoprotein , which does not bind FMN . - pubmed
Differential dopamine - induced prolactin mRNA levels in various prolactin - secreting cell ( sub)populations . - pubmed
We have examined the effects of dopamine on prolactin gene expression using quantitative in - situ hybridization histochemistry in different pituitary cell ( sub)populations separated according to their density on a discontinuous Percoll gradient . - pubmed
Administration of dopamine resulted in a drastic reduction in hybridization of 35S - labelled DNA probe complementary to prolactin mRNA in total pituitary cells and in lactotrophs with low density . - pubmed
In contrast , dopamine significantly stimulated mRNA accumulation in prolactin - secreting cells with high density compared with other cell layers . - pubmed
Prolactin - secreting cells with high and low density clearly show functional heterogeneity in their response to dopamine . - pubmed
In nonstripped colon , the action of serotonin ( 5 - hydroxytrytamine [ 5 - HT ] ) on short - circuit current was inhibited by a factor of 60 % by tetrodotoxin ( P less than 0.05 ; t test ) and was also inhibited by the 5HT2 antagonist ketanserin ( p less than 0.05 vs serotonin ; t test ) and the 5 - HT3 antagonist ICS 205 - 930 ( p less than 0.05 vs serotonin , t test ) . - pubmed
Surface TM activity was increased by active phorbol esters ( 10(-8 ) M , 24 - 48 h ) , analogs of cAMP ( 1 - 10 mM , 4 h ) , and forskolin ( 10(-5 ) M , 24 - 48 h ) . - pubmed
Up - regulation of TM in HUVECs by 4 beta - phorbol 12 - myristate 13 - acetate ( PMA ) and dibutyryl cAMP ( dBcAMP ) was due to de novo synthesis of TM protein resulting from increased TM mRNA levels . - pubmed
In addition , PMA effects on surface TM activity are biphasic , with an initial reduction followed by a significant enhancement . - pubmed
Continuous exposure to oestradiol-17 beta ( 1.8 nmol / l ) for 9 days in serum - free medium resulted in a decrease in cytosolic oestrogen receptors with an associated nuclear accumulation of oestrogen receptors . - pubmed
Thus , as previously reported for vaginal epithelial cells , oestrogen , despite its stimulation of specific product synthesis ( progestin receptors ) , did not increase proliferation of endometrial luminal epithelial cells in this culture system . - pubmed
Without SA or CA , GM was not absorbed . - pubmed
However , GM absorption was marked when 90 mg of solid SA or CA was added ( the bioavailability of GM was 58 % with SA , and 59 % with CA ) . - pubmed
The enhancing effect of SA or CA ( 30 mg ) in solid or aqueous solution form on GM absorption was evaluated using the Type I suppository . - pubmed
In the case of SA , the highest plasma GM level ( Cmax 15.3 + /- 1.7 micrograms / ml , AUC0 - 4 27.3 + /- 3.9 h.micrograms/ml ) was obtained following coadministration of powdered GM and SA ; the plasma GM level ( Cmax 1.5 + /- 0.6 micrograms / ml , AUC0 - 4 3.0 + /- 1.3 h.micrograms/ml ) following the administration of a solution of GM and SA was significantly decreased as compared with the results using the powdered form . - pubmed
A marked increase in the enhancing effect of SA on the rectal GM absorption was found following use of Type II suppositories when GM and SA were coadministered in solution form . - pubmed
However , the GM absorption after coadministration of GM and CA using Type II suppositories was not significantly different from the absorption resulting from use of Type I suppositories . - pubmed
Our results suggest that the form and concentration of drug should not be ignored in evaluating the enhancing effects of SA or CA on the rectal absorption of poorly absorbed drugs such as GM . - pubmed
Arecoline and arecaidine propargyl ester ( APE ) as well as their corresponding N - demethyl derivatives , guvacoline ( norarecoline ) and guvacine propargyl ester , acted as full agonists at both atrial and ileal muscarinic receptors ( range of pD2 - values 6.09 - 8.07 ) . - pubmed
Treatment of infected L cells with 10 micrograms / ml cytochalasin B ( CB ) was found to promote a rapid relocalization of viral glycoproteins on the cell surface . - pubmed
Acetaminophen as a cosubstrate and inhibitor of prostaglandin H synthase . - pubmed
At the concentrations tested , NOP , NPD , OPD , MPD and ANL were active in one or more assays . - pubmed
A panel of 10 FITC - labelled lectins ( MPA , PNA , ConA , DBA , SBA , RCA-120 , WGA , UEA , GS - I , GS - II+ ) was applied to cryosections of seven specimens of normal urothelium . - pubmed
MPA , RCA , UEA and GS - I showed the most marked increase in fluorescence intensity from basal to superficial cells as observed microscopically and quantified by flow cytometry . - pubmed
The purification led to an homogeneous hydrogenase preparation with a specific activity of 269 U / mg protein ( methylene blue reduction ) and a yield of 45 % . - pubmed
During purification and storage the enzyme was optimally stabilized by the presence of 0.2 mM MnCl2 . - pubmed
The acceptors reduced include viologen dyes , flavins , quinones , cytochrome c , methylene blue , 2,6 - dichlorophenolindophenol , phenazine methosulfate and ferricyanide . - pubmed
eutrophus H16 it was demonstrated that this type of hydrogenase in its solubilized , purified state is able to catalyze also the reverse reaction , the H2 evolution from reduced methyl viologen . - pubmed
The effect of d - and l - norepinephrine on rat cardiac phosphorylase activation . - pubmed
Alanine dehydrogenase activity and growth yield ( grams of cells / mole of methane ) were highest when the organism was cultured with excess ammonia , whereas growth yield was lower and glutamine synthetase was maximal when ammonia was limiting . - pubmed
These findings support the view that liver HBcAg and serum HBeAg are markers of virus replication , and demonstrate that active liver disease in HBsAg - positive patients may occur with or without such markers of replication . - pubmed
We report our observed change in the pattern of progression of septicemia to meningitis and death in nonimmunized SS children who were not receiving prophylactic penicillin in the face of a persistently high incidence of pneumococcal disease . - pubmed
In the presence of catalase , the amounts of chromium released by phagocytosing and resting macrophages were similar ; in the presence of superoxide dismutase and cytochrome c , the amount of chromium released by phagocytosing macrophages was 13 - 35 % less than the amount of chromium released by macrophages incubated without the antioxidants . - pubmed
The aim of this study was to assess the behavior of fasting serum glucose , C - peptide levels and OGTT in pancreatic cancer follow - up . - pubmed
[ Evaluation of serum adenosine deaminase as a prognostic marker in the treatment of human immunodeficiency virus infection with zidovudine ] . - pubmed
Therapy with AZT decreased ADA activity : 21.6 + /- 5.4 vs. 15.2 + /- 4.3 U / l ( p & lt ; 0.001 ) and correlated with an increase in CD4 counts : 187 + /- 105 vs. 353 + /- 145 / mm3 ( p & lt ; 0.001 ) and in CD4 / CD8 ratio : 0.188 + /- 0.10 vs. 0.382 + /- 0.18 ( p & lt ; 0.001 ) . - pubmed
Use of a polymorphic dinucleotide repeat sequence to detect non - blastomeric contamination of the polymerase chain reaction in biopsy samples for preimplantation diagnosis . - pubmed
MEASUREMENTS : Serum thyroid hormone levels , serum thyrotropin receptor antibody titer , and thyroidal radioiodine uptake . - pubmed
L - NAME significantly decreased sodium excretion ( 1.38 + /- 0.41 to 0.36 + /- 0.14 microEq / min and 1.19 + /- 0.46 to 0.30 + /- 0.05 microEq / min , respectively ) , fractional excretion of lithium ( 25.7 + /- 1.7 % to 16.7 + /- 2.3 % and 25.6 + /- 4.0 % to 18.2 + /- 1.7 % ) , and renal interstitial hydrostatic pressure ( 6.4 + /- 1.4 to 3.2 + /- 0.9 mm Hg and 6.3 + /- 1.8 to 2.7 + /- 0.9 mm Hg ) in servocontrolled and nonservocontrolled groups . - pubmed
After injection of UK , NEP decreased significantly and urinary kinins , cGMP , urine volume ( UV ) and urinary sodium excretion ( UNaV ) increased significantly . - pubmed
With a pretreatment of L - NMMA , injected UK decreased NEP and increased kinins , while urinary cGMP , UV and UNaV did not increase . - pubmed
Moreover , changes in urinary cGMP do not reflect the changes in plasma ANP , but rather , those in NO under this condition . - pubmed
Focal accumulations of mononuclear cells containing IL-2 producing cells were readily detected as early as 2 - 3 h following local challenge with hapten in animals primed at skin but not at buccal sites . - pubmed
Known as osmotic stress protein 94 , or Osp94 , this 2935 - base pair cDNA encodes an 838 - amino acid protein that shows greatest homology to the recently discovered hsp110 / SSE gene subfamily . - pubmed
Like the hsps , Osp94 has a putative amino - terminal ATP - binding domain and a putative carboxyl - terminal peptide - binding domain . - pubmed
The in vitro translated Osp94 product migrated as a 105 - 110 - kDa protein on SDS - polyacrylamide gel electrophoresis . - pubmed
Treatment of woodchuck hepatitis virus infection in vivo with 2 ' , - 3 '- dideoxycytidine ( ddC ) and 2 ',- 3 '- dideoxycytidine monophosphate coupled to lactosaminated human serum albumin ( L - HSA ddCMP ) . - pubmed
The present study examined the effects of lesions of the central nucleus of the amygdala ( CeA ) on the acquisition of a new response with conditioned reinforcement ( CR ) and its potentiation by intra - accumbens infusions of d - amphetamine ( 1 , 3 , 10 , and 20 microg / microl ) . - pubmed
Remifentanil is a new synthetic opioid with direct action on mu - opioid receptors . - pubmed
The hydrolysis of remifentanil produces a metabolite with very weak opioid receptor activity that does not contribute to the effects of remifentanil . - pubmed
The authors examined the effect of four different kinds of contrast media ( ionic / non - ionic , monomer / dimer ) on the activation of the complement ( C ) system ( haemolytic activity and anaphylatoxin generation ) in vitro . - pubmed
In addition , the authors compared the effect of contrast media on inulin - mediated generation of the anaphylatoxin derivative C3a des Arg in sera from urticarial reactors and their non - reacting controls . - pubmed
No significant difference between the urticarial reactors and non - reactors in the inhibition of C3a des Arg generation was observed . - pubmed
These include , amongst others , the platelet - derived growth factor receptor , the fibroblast growth factor receptor , and the nonreceptor c - Src tyrosine kinase . - pubmed
Leech immunocytes contain proopiomelanocortin : nitric oxide mediates hemolymph proopiomelanocortin processing . - pubmed
No dibasic amino acid residues were found at the C terminus of the gamma - and beta - MSH peptides . - pubmed
In contrast , the leech alpha - MSH was flanked at its C - terminal by the Gly - Arg - Lys amidation signal . - pubmed
ACTH and corticotropin - like intermediary pituitary peptide were also C - terminally flanked by dibasic amino acid residues . - pubmed
Nonsteroidal anti - inflammatory drugs may delay the repair of gastric mucosa by suppressing prostaglandin - mediated increase of hepatocyte growth factor production . - pubmed
Prostaglandins ( PGs ) , hepatocyte growth factor ( HGF ) , and induction of cyclooxygenase ( PG synthetase , COX ) play important roles in the repair process of gastric mucosa . - pubmed
Among various potential stimulants tested , interleukin-1 beta ( IL-1 beta ) dramatically increased PGE2 production and significantly stimulated HGF production . - pubmed
IND significantly reduced both basal and IL-1 beta - induced PGE2 release and HGF production . - pubmed
This study determined whether estradiol regulates insulin - like growth factor ( IGF )- I and IGF binding proteins ( IGFBPs ) in the pituitary gland , hypophyseal stalk median eminence ( SME ) , and circulation concomitantly with effects on LH . - pubmed
Estradiol suppressed serum LH to a greater extent during anestrus than after onset of the breeding season ( Days 60 and 75 ) . - pubmed
Amounts of mRNA for LHbeta subunit were decreased by estradiol , but mRNA for alpha and FSHbeta subunits were not affected . - pubmed
Estradiol increased serum IGF - I , IGFBP-3 , and IGFBP-4 throughout the treatment period , but it did not influence other IGFBPs in serum . - pubmed
In response to estradiol , pituitary IGFBP-2 tended to increase and mRNA for IGFBP-2 increased twofold . - pubmed
Other IGFBPs in the pituitary gland were not influenced by estradiol . - pubmed
In the SME , IGFBP-2 , IGFBP-5 , and the 40 - kDa IGFBP-3 were increased by estradiol . - pubmed
Estradiol influences on gonadotroph function may be mediated by alterations in the IGF system . - pubmed
These results suggest that the etidronate treatment for 2 weeks beforehand influenced the effects of subsequent exercise training on maintaining the BMD in the proximal femur and the trabecular bone area of the tibia . - pubmed
Maximal positive GluR2 promoter activity required both Sp1 and NRF-1 cis elements and an interelement nucleotide bridge sequence . - pubmed
The deleterious effects of the abasic mutations are not relieved by lowering the temperature , by increasing the concentration of monovalent or divalent metal ions , or by adding polyamines , in contrast to effects observed with protein enzymes and large RNA enzymes . - pubmed
Biochemical analysis of the Cas - Br - E Env isoforms indicated that they result from differential processing of N - linked sugars . - pubmed
Methylene group selectivity data showed only minor differences in the stationary - phase characteristics between the small- and large - pore size C18 columns . - pubmed
The true eluting power of micellar mobile phases was revealed with wide - pore stationary phases and was demonstrated by the separation and elution of an extended series of alkylphenones on C18 columns . - pubmed
The expression of conglutin gamma was studied by partial amino acid sequencing of the mature seed protein and by nucleotide sequencing of reverse transcriptase - polymerase chain reaction products from various tissues during the plant life cycle . - pubmed
The inhibitory effect of CART peptide 55 - 102 on Ca ( 2 + ) signaling is likely mediated by an inhibition of L - type voltage - gated Ca ( 2 + ) channel activity via a G - protein - dependent pathway . - pubmed
These results indicate that voltage - gated Ca ( 2 + ) channels in hippocampal neurons are targets for CART peptide 55 - 102 and suggest that CART peptides may be important in physiology and behavior mediated by the hippocampus , such as certain forms of learning and memory . - pubmed
RESULTS : Tx had postabsorptive and insulin - stimulated glucose , leucine , and free fatty acid metabolism , as well as IMCL content , similar to that of CON . - pubmed
A defective insulin - dependent suppression of the endogenous leucine flux ( index of proteolysis ) was also evident during the clamp in D - Tx . - pubmed
CONCLUSIONS : Heart transplantation , notwithstanding the immunosuppressive therapy , was characterized by a normal postabsorptive and insulin - stimulated glucose , leucine , and free fatty acid metabolism in Tx . - pubmed
A natural gradient experiment with dissolved MTBE - containing gasoline in the shallow , aerobic sand aquifer at Canadian Forces Base ( CFB ) Borden ( Ontario , Canada ) from 1988 to 1996 suggested that biodegradation was the main cause of attenuation for MTBE within the aquifer . - pubmed
Quinoline resistance in malaria is frequently compared with P - glycoprotein - mediated multidrug resistance ( mdr ) in mammalian cells . - pubmed
Genetic analysis of putative quinoline resistance genes did not suggest an association between known point mutations in pfcrt and pfmdr1 and NP30 sensitization activity . - pubmed
Class IC drugs ( ethacizine , disopyramide ) caused worsening of qualitative parameters of SA ECG and appearance of ventricular late potentials . - pubmed
Semen samples and serum for reproductive hormones ( testosterone , luteinizing hormone and follicle - stimulating hormone ) were collected at baseline , after 3 months and at the end of treatment . - pubmed
[ The duration of effects of a zinc insulin protaminate ] . - pubmed
We propose a new mechanism of metabolic compensation of seasonal temperature variations - cold acclimation results in changes in the kinetic and thermodynamic properties of LDH in a way that favours aerobic metabolism through reduction of the competition of LDH for pyruvate in normoxic conditions . - pubmed
Ethanol shows antiketonaemic effects changing the cellular redox potential , inhibiting beta - oxidation of fatty acids , stimulating the release of insulin and inhibiting the release of its antagonist . - pubmed
Pigpen is a cellular binding protein of therapeutic oligonucleotides . - pubmed
Although co - localization of pigpen with phosphorothioate ( PS ) ON was not observed for the first hour after ON uptake , co - localization was observed 8 h later . - pubmed
One - pot synthesis of large FePt nanoparticles from metal salts and their thermal stability . - pubmed
One - pot synthesis of FePt nanoparticles larger than 5 nm with controlled composition has been developed by the polyol reduction of platinum acetylacetonate and iron acetylacetonate in excess ligands . - pubmed
When mitochondrial oxidases were blocked , illumination of the algae reversed the effect of the inhibitors on the ATP and glucose 6 - phosphate concentrations . - pubmed
We conclude that hyperhomocysteinemia enhances susceptibility to arterial thrombosis through a mechanism that is not caused by loss of endothelium - derived nitric oxide but may involve oxidative stress and impairment of the protein C anticoagulant pathway . - pubmed
SPK activity assay was used to evaluate the effect of alphastatin on ECs . - pubmed
CONCLUSION : Downregulating ECs SPK activity may be one of the mechanisms that alphastatin inhibits gastric cancer angiogenesis . - pubmed
Dual - specificity phosphatases ( DSPs ) , which dephosphorylate proteins at Ser / Thr as well as Tyr residues , are thought to be involved in critical signaling events such as control of MAP kinases ( MAPKs ) . - pubmed
LDP-4 is composed of 211 amino acids with a predicted molecular mass of 23.9 - kDa . - pubmed
Here we describe the crystal structure of GlpG in a more open conformation , where the capping loop L5 has been lifted , exposing the previously buried and catalytically essential Ser-201 to outside aqueous solution . - pubmed
The loop movement also destabilizes a hydrophobic side chain ( Phe-245 ) previously buried between transmembrane helices S2 and S5 and creates a side portal from the lipid to protease active site . - pubmed
BAEP measurements recorded at 85 dB both at entry into and after the study were not significantly different among the groups , although a sufficient response to iron treatment was achieved in iron - deficient groups ( Group I and Group II ) . - pubmed
A selective CRF1 receptor antagonist ( NBI27914 ) or vehicle was administered systemically ( i.p . ) - pubmed
Most of these cells exhibited activation evidenced by an increased reactive oxygen species generation and high expression of the Gr1 +/ Mac1 + markers . - pubmed
An amine - targeted iTRAQ tag labels tryptic peptides generated from the proteins and protease cleavage products of secreted proteins , as well as protein domains shed from the cell membrane or pericellular matrix of protease - transfected cells that have accumulated in conditioned medium ; a second iTRAQ tag is used for control cells . - pubmed
A conditional logistic regression model was used to identify risk factors for MRSA bacteraemia compared with methicillin - sensitive SAB and no bacteraemia in unmatched ( 1:1 ) and matched ( 1:4 ) nested case - control analyses , respectively . - pubmed
Phase 2 study of gemcitabine and irinotecan in metastatic breast cancer with correlatives to determine topoisomerase I localization as a predictor of response . - pubmed
BACKGROUND : Gemcitabine incorporation into DNA enhances cleavage complexes in vitro when combined with topoisomerase I inhibitors and demonstrates synergy in cancer cells when given with irinotecan . - pubmed
Correlative studies included characterization of cellular levels and nuclear distribution of topoisomerase I and pharmacokinetic analysis of gemcitabine and irinotecan . - pubmed
TTR was also down - regulated by the commercial penta - BDE mixture , DE-71 . - pubmed
In contrast , neither DE-71 , nor BDE-47 , -99 , or -100 altered the mRNA levels of the TRs or nAChR alpha-7 . - pubmed
Deletion of Fphog1 , a HOG - type MAP kinase gene resulted in further increases in FUM1 and FUM8 expression under nitrogen starvation ( absence of any N - source ) conditions . - pubmed
Fumonisin B1 ( FB1 ) production paralleled with increased FUM gene expression : significant amounts of FB1 were measured in culture filtrates of the DeltaFphog1 deleted mutant after five days culturing , whereas only traces of FB1 could be detected in filtrates of the wild type and the restored strain ( R1 ) complemented with the wild - type Fphog1 - 24 gene . - pubmed
The up - regulation of fumonisin biosynthesis genes in the DeltaFphog1 mutant could be explained by the increased sensitivity of these strains to N - starvation stress that appears in the absence of an intact HOG - type MAPK pathway . - pubmed
In addition , HMC05 significantly inhibited production of reactive oxygen species ( ROS ) and nuclear factor ( NF )- kappaB activation by TNF - alpha . - pubmed
Those inhibitory effects of HMC05 ( 1 - 10 mug mL(-1 ) ) on the TNF - alpha - induced inflammatory event was similar to those of berberine ( 1 - 10 muM ) , which is a major component of HMC05 and one of herbal compounds known to have vasorelaxing and lipid - lowering activities . - pubmed
Role of the AMPKgamma3 isoform in hypoxia - stimulated glucose transport in glycolytic skeletal muscle . - pubmed
Skeletal muscle glucose transport is regulated via the canonical insulin - signaling cascade as well as by energy - sensing signals . - pubmed
5 '- AMP - activated protein kinase ( AMPK ) has been implicated in the energy status regulation of glucose transport . - pubmed
Taken together , we provide direct evidence for a role of the AMPKgamma3 isoform in hypoxia - mediated glucose transport in glycolytic muscle . - pubmed
Biochemical characteristics in term of quantities of protein and nitric oxide ( NO ) did not differ significantly , whereas the quantity of glucose , acid phosphatase ( ACP ) and vitamin E were significantly higher in the T(2 ) group . - pubmed
CD8(+ ) T cells are activated upon presentation of antigens from the cytosol . - pubmed
Physiological observations upon a larval Eustrongylides ; influence of oxygen tension on the aerobic and post - anaerobic oxygen consumption . - pubmed
The in vitro exposure of kidney proximal tubular cells to physiological concentrations of C - peptide activates extracellular signal - regulated kinase , phosphatidylinositol 3 - kinase , protein kinase C , elevates intracellular calcium , and stimulates transcription factors NF - kappaB and peroxisome proliferator - activated receptor - gamma . - pubmed
Immersion in a simulated body solution ( SBF ) solution was used to evaluate the bioactivity of the plasma - treated PET / TiO ( 2 ) samples in vitro . - pubmed
Analytical and microstructural investigations of SBF results , showed considerable higher rates of apatite formation on the plasma - treated PET / TiO ( 2 ) compared to the untreated films . - pubmed
The BH3 - only protein Bad confers breast cancer taxane sensitivity through a nonapoptotic mechanism . - pubmed
Unexpectedly , Bad did not induce the mitochondrial apoptotic machinery in response to taxane treatment . - pubmed
As dividing cells are the targets of taxane therapy , Bad - stimulated proliferation may be a marker of taxane sensitivity . - pubmed
They also suggest that cells expressing Bad are more sensitive to taxanes because of their altered cell cycle dynamics and reveal a clinically relevant proliferative role of Bad in breast cancer . - pubmed
After curcumin treatment , BAX and BAD were up - regulated , BCL-2 , BCL - X ( L ) and XIAP were down - regulated . - pubmed
Apoptotic cell death induced by curcumin was significantly reversed by pretreatment with ROS scavenger or caspase-8 inhibitor . - pubmed
RESULTS : The T124A mutation alone and the combination T124A / L101I were more frequent in raltegravir - failing patients than in INI - naive patients ( 39.3 % versus 24.5 % , respectively , P = 0.005 for T124A and 20.2 % versus 10.0 % , respectively , P = 0.008 for T124A / L101I ) . - pubmed
The optimal temperature was in the range 45 - 55 C. All three proteases were active towards azokeratin and were inhibited by EDTA , suggesting that they are keratinolytic metalloproteases . - pubmed
The proteolytic activity of K12 was stimulated by organic solvents and the detergent SDS , suggesting its potential application for detergent formulations and peptide synthesis . - pubmed
The number of immature flukes was positively correlated with values of aspartate aminotransferase ( AST ) , gamma - glutamyltransferase ( GGT ) , LDH , GLDH , urea and triglycerides . - pubmed
The number of migratory lesions was positively correlated with GGT , GLDH , globulin and urea values . - pubmed
Thermo - sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells . - pubmed
Transient receptor potential channels ( TRPs ) regulate tumor growth via calcium - dependent mechanisms . - pubmed
The ( thermosensitive ) capsaicin receptor TRPV1 is overexpressed in numerous highly aggressive cancers . - pubmed
Intracellular free Ca ( 2 + ) ( [ Ca ( 2 +)]( i ) ) was measured by fura-2 ; TRPV1 channel currents by the planar patch - clamp technique . - pubmed
Pharmacological blockade of TRPs by La ( 3 + ) ( 100 muM ) or by ruthenium - red ( RuR ) or by capsazepine ( CPZ ) ( both at 10 muM ) suppressed the capsaicin ( CAP)- or heat - stimulated increase of [ Ca ( 2 +)]( i ) in NET cells . - pubmed
Furthermore , CAP ( 10 muM ) stimulated CgA secretion , which was inhibited by CPZ or by RuR ( both 10 muM ) . - pubmed
La ( 3 + ) potently reduced both stimulated and the basal CgA secretion . - pubmed
Activation of TRPV1 translates into changes of intracellular Ca ( 2 + ) , a known mechanism triggering the secretion of CgA. - pubmed
In both WT and synapsin DKO beta - cells the Ca ( 2 + ) current peaked at 0 mV and measured peak current ( I(p ) ) and net charge ( Q ) were of similar magnitude . - pubmed
MEASUREMENTS : The primary outcomes were 25 -( OH ) D and PTH levels at 6 and 12 months . - pubmed
Parathyroid hormone levels at 12 months decreased with an increasing dose of vitamin D ( 3 ) ( P = 0.012 ) . - pubmed
Study of surface activity of piroxicam at the interface of palm oil esters and various aqueous phases . - pubmed
The aim of this study was , firstly , to assess the surface and interfacial tension behaviour of newly synthesised palm oil esters and various pH phosphate buffers . - pubmed
The study revealed that piroxicam has no effect on surface tension values of all pH phosphate buffers and palm oil esters . - pubmed
The arginine methyltransferase PRMT5 - MEP50 is required for embryogenesis and is misregulated in many cancers . - pubmed
However , the mechanism by which PRMT5 utilizes MEP50 to discriminate substrates and to specifically methylate target arginines is unclear . - pubmed
HO-1 , a microsomal enzyme , catalyzes the breakdown of pro - oxidant heme , which is released from heme proteins to equimolar quantities of iron , carbon monoxide , and biliverdin . - pubmed
Biliverdin is converted to bilirubin by biliverdin reductase . - pubmed
The beneficial effects of HO-1 expression are not merely due to heme degradation but are also attributed to the cytoprotective properties of the byproducts of the reaction . - pubmed
Bone - seeking tracers like 99mTc - DPD and pyrophosphate are beginning to have a role in imaging transthyretin cardiac amyloidosis . - pubmed
GROWING POINTS AND FURTHER RESEARCH : 99mTc - DPD has a high sensitivity and specificity to cardiac transthyretin amyloid deposits and its role in early diagnosis of this condition is under investigation . - pubmed
2 -( 2 - Phenylmorpholin-4 - yl ) pyrimidin-4 ( 3H )- ones ; a new class of potent , selective and orally active glycogen synthase kinase-3beta inhibitors . - pubmed
A series of 2 -( 2 - phenylmorpholin-4 - yl ) pyrimidin-4 ( 3H )- ones was synthesized and examined for their inhibitory activity against glycogen synthase kinase-3beta ( GSK-3beta ) . - pubmed
We hypothesized that an aldosterone excess was associated with abnormalities in urinary proteome , specific for PA subtypes . - pubmed
Single nucleotide polymorphisms ( SNPs ) in the regulatory regions of TPH2 gene may affect gene expression and biosynthesis of 5 - HT triggering to various neuropsychiatric disorders related to 5 - HT dysfunction . - pubmed
Rab9 plays a vital role in regulating the transport of mannose 6 - phosphate receptors from late endosomes to the trans - Golgi network through interactions with various effectors . - pubmed
Here , we report the crystal structure of GTP - bound Rab9A in complex with the Rab - binding domain ( RBD ) of the effector RUTBC2 . - pubmed
Dietary GI and GL were independently associated with risk for endometrial cancer but carbohydrate intake was unrelated to risk . - pubmed
This study suggests that intake of high GL or GI foods , but not carbohydrates per se , may increase risk for endometrial cancer . - pubmed
PMA primed PBTLs for polarization under flow , with protein kinase C ( PKC )- delta enriched in the leading edge , PKC - betaI in the microtubule organizing center , and PKC - betaII in the uropod and peripheral region . - pubmed
RESULTS : Serum osteocalcin level was independently associated with menopausal status , bone mineral density , calcium , phosphate , alkaline phosphatase , fasting blood glucose , triglyceride , low - density lipoprotein cholesterol , alanine aminotransferase , gamma - glutamyltransferase , and NAFLD . - pubmed
The ER stress response is an adaptive mechanism that allows tumor cells to survive in the harsh growth conditions inherent to the tumor milieu such as low oxygen ( hypoxia ) , low pH and low levels of glucose . - pubmed
Following collapse of the GC , memory T cells persisted in the outer follicle where they scanned CD169(+ ) subcapsular sinus macrophages . - pubmed
These data suggest that Tfh cells integrate their antigen - experience history to focus T cell help within the GC during primary responses but act rapidly to provide systemic T cell help after re - exposure to the antigen . - pubmed
Qiliqiangxin Protects Against Cardiac Ischemia - Reperfusion Injury via Activation of the mTOR Pathway . - pubmed
Expression levels of phospho - mTOR ( Ser2448 ) , mTOR , phospho-4EBP ( Ser65 ) , 4EBP , phospho - Akt ( Ser473 ) and Akt were detected by Western blotting . - pubmed
Moreover , western blotting analysis demonstrated that QL activated the mTOR pathway , while mTOR inhibition via Rapamycin abolished the protective effects of QL against I / R injury . - pubmed
To engineer biocompatible materials from beta - cyclodextrin ( beta - CD ) , in this study , we synthesized acetalated beta - CDs ( Ac - betaCDs ) by one - pot acetalation using 2 - ethoxypropene as an acetonation reagent , which can be further processed into nanoparticles ( NPs ) via the emulsion technique . - pubmed
We also demonstrated increased serotonin fibers and synaptophysin in direct contact with motor neurons . - pubmed
We showed that DHA significantly upregulates miR-21 and downregulates PTEN in corticospinal neurons . - pubmed
Downregulation of PTEN and upregulation of phosphorylated AKT by DHA were also seen in primary cortical neuron cultures and were accompanied by increased neurite outgrowth . - pubmed
We show that DHA significantly upregulates miR-21 and phosphorylated AKT , and downregulates phosphatase and tensin homolog ( PTEN ) , which is involved in suppressing anatomical plasticity , in corticospinal neurons and in primary cortical neuron cultures . - pubmed
Finally , they support the hypothesis that ARFs are a crucial component of the auxin signaling pathway , which regulates a wide range of physiological processes . - pubmed
Among the resistant haplotypes , allelic progression involving an additional cis - acting mutation and gene duplication evolved a CYP9M10 haplotype associated with extremely high transcription and strong pyrethroid resistance . - pubmed
To achieve the same effect , at least 500 mug / mL Cur - NP alone was needed . - pubmed
Hb Constant Spring ( Hb CS , HBA2 : c.427 T & gt ; C ) is a common nondeletional alpha - thalassemia ( alpha - thal ) that results from a nucleotide substitution at the termination codon of the alpha2 - globin gene . - pubmed
DNA sequencing of the alpha - globin genes found that both partners were Hb CS carriers and the fetus was an Hb CS homozygote . - pubmed
The following discussion , derived from an extensive literature search using the PubMed database , applying the terms incretin , GLP-1 , exenatide , liraglutide , albiglutide , dulaglutide , lixisenatide , semaglutide , and taspoglutide , provides a comprehensive review of existing and upcoming molecules in the GLP-1 RA class in terms of their structure , pharmacological profiles , efficacy , safety , and convenience . - pubmed
Among the standard functionals , omegaB97X - D and SOGGA11X yield accurate charge reorganization energies in comparison with IP - EOM - CCSD values . - pubmed
Together , our results reveal that S1PR1 contributes to survival and growth mechanisms in injured RGCs by regulating the mammalian target of rapamycin pathway . - pubmed
Synthesis , cytotoxicity and carbonic anhydrase inhibitory activities of new pyrazolines . - pubmed
A series of polymethoxylated - pyrazoline benzene sulfonamides were synthesized , investigated for their cytotoxic activities on tumor and non - tumor cell lines and inhibitory effects on carbonic anhydrase isoenzymes ( hCA I and hCA II ) . - pubmed
Although tumor selectivity ( TS ) of the compounds were less than the reference compounds 5 - Fluorouracil and Melphalan , trimethoxy derivatives 4 , 5 , and 6 were more selective than dimethoxy derivatives 2 and 3 as judged by the cytotoxicity assay with the cells both types originated from the gingival tissue . - pubmed
The compound 6 ( 4 -[ 3 -( 4 - methoxyphenyl )- 5 -( 3,4,5 - trimethoxyphenyl )- 4,5 - dihydro-1H - pyrazol-1 - yl ] benzene sulfonamide ) showed the highest TS values and can be considered as a lead molecule of the series for further investigations . - pubmed
All compounds synthesized showed superior CA inhibitory activity than the reference compound acetazolamide on hCA I , and II isoenzymes , with inhibition constants in the range of 26.5 - 55.5 nM against hCA I and of 18.9 - 28.8 nM against hCA II , respectively . - pubmed
KAT6A - deficient CD8(+ ) T cells downregulated surface CD8 co - receptor expression during clonal expansion , a finding linked to reduced Cd8alpha transcripts and histone - H3 lysine 9 acetylation of the Cd8 locus . - pubmed
Moreover , the axons of identified PV+/Scgn+ interneurons preferentially targeted the somata of SPNs of the so - called ' direct pathway ' , whereas PV+/Scgn- interneurons preferentially targeted ' indirect pathway ' SPNs . - pubmed
Intestinal bile acid receptors are key regulators of glucose homeostasis . - pubmed
Bile acids bind and modulate the activity of transmembrane and nuclear receptors ( NR ) . - pubmed
Among these receptors , the G - protein - coupled bile acid receptor 1 ( TGR5 ) and the NR farnesoid X receptor ( FXR ) are implicated in the regulation of bile acid , lipid , glucose and energy homeostasis . - pubmed
More precisely , recent studies have shown that FXR is important for glucose homeostasis in particular in metabolic disorders such as T2D and obesity . - pubmed
This review highlights the growing importance of the bile acid receptors TGR5 and FXR in the intestine as key regulators of glucose metabolism and their potential as therapeutic targets . - pubmed
The concordances of the GenoType MTBDRplus assay and the conventional DST for the detection of RIF and INH susceptibility were 80 % ( 8/10 ) and 86.2 % ( 25/29 ) , respectively . - pubmed
For INH - resistant isolates , the highest mutation rate ( 88.8 % ) related to a katG mutation ( Codon S315T1 ) . - pubmed
CONCLUSION : The findings of this study revealed that the GenoType MTBDRplus assay has high sensitivity and specificity for the detection of RIF and INH resistance . - pubmed
The crystal structures of title hydrogen - bonded co - crystals , 2C12H16O3 C12H10N2 , ( I ) , and 2C13H18O3 C12H10N2 , ( II ) , have been determined at 93 K. In ( I ) , the asymmetric unit consists of one 4 -( n - pent - yloxy ) benzoic acid mol - ecule and one half - mol - ecule of ( E )- 1,2 - bis -( pyridin-4 - yl ) ethene , which lies about an inversion centre . - pubmed
The asymmetric unit of ( II ) comprises two crystallographically independent 4 -( n - hex - yloxy ) benzoic acid mol - ecules and one 1,2 - bis -( pyridin-4 - yl ) ethene mol - ecule . - pubmed
Annexin V , a protein with high affinity to phosphatidylserine ( PS ) in a calcium dependent manner , has been widely used to probe apoptosis . - pubmed
Analysis of Novel Interactions between Components of the Selenocysteine Biosynthesis Pathway , SEPHS1 , SEPHS2 , SEPSECS , and SECp43 . - pubmed
The SECp43 structural model presented here constitutes the basis of future exploration of the protein - protein interactions among early components of the selenocysteine biosynthesis and incorporation pathway . - pubmed
Changes in total , HDL- , and LDL - cholesterol , and urine 8 - isoprostane levels did not differ between the two groups . - pubmed
METHODS : Pulmonary function testing results were compared during apnea induced by hyperventilation ( CO2 washout ) vs. intravenous propofol ( 1 dose 10 mg / kg ) . - pubmed
The 16S rRNA amplicon sequencing analysis revealed differences in beta diversity with a significant reduction in the relative abundances of short - chain fatty acid ( SCFA ) producers such as Oscillospira and Ruminococcaceae due to BPA exposure . - pubmed
The principal steps of intracellular cytokine staining are as follows : First , cells are activated for a few hours using either a specific peptide or a non - specific activation cocktail . - pubmed
Brefeldin A ) is added to retain the cytokines within the cell . - pubmed
Ryanodine receptors ( RyRs ) , the large homotetrameric protein complexes , regulate the release of calcium from intracellular stores into the cytosol and play vital roles in the excitation - contraction coupling of cells . - pubmed
The changes in RGC spontaneous firing and membrane potential could be reversed by the tyrosine kinase inhibitor PP2 , suggesting that EphB / ephrinB reverse signaling activation induced RGC hyperexcitability . - pubmed
Intravitreal pre - injection of either LY367385 or MPEP , selective mGluR1 and mGluR5 antagonists , also blocked the changes in RGC spontaneous firing and membrane potential . - pubmed
Furthermore , intravitreal pre - injection of the mixture of L - NAME ( an NO synthase inhibitor ) and XPro1595 ( a selective inhibitor of soluble TNF - alpha ) could reduce the EphB2 - Fc injection induced increase in RGC firing , suggesting that Muller cells might be involved in EphB / ephrinB reverse signaling activation induced change in RGC hyperexcitability . - pubmed
In addition , LY367385 / MPEP reduced the numbers of TUNEL - positive RGCs both in EphB2 - Fc injected and COH retinas . - pubmed
Neratinib is a tyrosine kinase inhibitor that has been approved by the US Food and Drug Administration for the treatment of breast cancer . - pubmed
This study was carried out to investigate the in vitro and in vivo metabolism of neratinib using an UHPLC - DAD - Q Exactive Orbitrap - MS instrument with dd - MS2 on - line data acquisition mode . - pubmed
Our findings suggested that neratinib was mainly metabolized via O - dealkylation ( M3 ) , oxygenation ( M8 ) , N - demethylation ( M10 ) , N - oxygenation ( M12 ) , GSH conjugation ( M1 , M2 , M4 , M5 , M6 and M7 ) and N - acetylcysteine conjugation ( M9 and M11 ) . - pubmed
Vascular hyperreactivity was accompanied by reduced nitric oxide ( NO ) , and was reversed by incubation with Dimethylsulfoxide , Catalase and Tempol . - pubmed
The NADPH oxidase subunit gp91phox was increased due to iron - overload , and incubation with angiotensin II type-1 receptor ( AT1 ) antagonist losartan not only reduced oxidative stress but also restored vascular function . - pubmed
Thus , we concluded that AT1 pathway plays a role in pulmonary vascular dysfunction by increasing oxidative stress and reducing NO bioavailability , thereby contributing to vascular remodeling and pulmonary hypertension of iron - overload . - pubmed
This finding should instigate future studies on the beneficial impacts of in vivo blockade of AT1 receptor under iron overload . - pubmed
savastanoi QS regulon consisted of only a few loci whereas in Etoletana many putative metabolic genes were regulated by QS , among which are several involved in carbohydrate metabolism . - pubmed
Response was evaluated using the 68Ga - PSMA - ligand - PET / CT or 99mTc - iPSMA - SPECT / CT diagnostic images and serum PSA levels before and after 177Lu - iPSMA treatment . - pubmed
The regulator of chromosome condensation 1 ( RCC1 ) is the nucleotide exchange factor for a GTPase called the Ras - related nuclear protein , and it is important for nucleo - plasmic transport , mitosis , nuclear membrane assembly , and control of chromatin agglutination during the S phase of mitosis in animals . - pubmed
Water depth , transparency ( SD ) , electric conductivity , and total nitrogen were the highest in spring , while temperature and total phosphorus were the highest in summer . - pubmed
During the spring period , Protozoa were positively correlated with conductivity , transparency ( SD ) , and total nitrogen . - pubmed
A 24 - week study demonstrated that sodium benzoate , a D - amino acid oxidase ( DAAO ) inhibitor , surpassed placebo in improving cognitive function in early - phase Alzheimer ' s disease ; however , benzoate did not excel placebo in another 6 - week study on BPSD . - pubmed
DAAO level , amino acids ( L - serine , D - serine , L - alanine , and D - alanine , glycine ) , and two antioxidants ( catalase , superoxide dismutase ) were assayed in peripheral blood . - pubmed
Results : After benzoate treatment , DAAO inhibition was correlated with ADAS - cog decrease ( p = 0.034 ) , while baseline DAAO level was correlated with baseline BEHAVE - AD score . - pubmed
HIF-1alpha - dependent miR-424 induction confers cisplatin resistance on bladder cancer cells through down - regulation of pro - apoptotic UNC5B and SIRT4 . - pubmed
RESULTS : In this study , we have found for the first time that cisplatin ( CDDP ) induces the expression of miR-424 in a HIF-1alpha - dependent manner under normoxia , and miR-424 plays a vital role in the regulation of CDDP resistance of bladder cancer cells in vitro . - pubmed
Moreover , knockdown of UNC5B or SIRT4 prohibited CDDP - mediated proteolytic cleavage of PARP and also decreased CDDP sensitivity of these cells . - pubmed
CONCLUSIONS : Collectively , our current results strongly suggest that miR-424 tightly participates in the acquisition / maintenance of CDDP - resistant phenotype of bladder cancer cells through down - regulation of its targets UNC5B and SIRT4 , and thus combination chemotherapy of CDDP plus HIF-1alpha / miR-424 inhibition might have a significant impact on hypoxic as well as normoxic bladder cancer cells . - pubmed
The ileum was processed for the evaluation of myeloperoxidase ( MPO ) , malondialdehyde ( MDA ) , and the protein expression of occludin and activated caspase-3 . - pubmed
RESULTS : Indomethacin elicited a significant intestinal damage , associated with a decrease in blood hemoglobin and an increase in tissue MPO , MDA and fecal calprotectin . - pubmed
In this setting , either the PAR1 agonist or PAR2 antagonist counteracted these changes , with the exception of MDA , which was unaffected . - pubmed
Indomethacin also decreased occludin and increased activated caspase-3 expression in ileal tissues . - pubmed
CONCLUSIONS : PAR2 is involved in the pathogenesis of indomethacin enteropathy , through pro - inflammatory mechanisms and an impairment of the intestinal epithelial barrier . - pubmed
Within the AMP group , increases in PA and decreases in NA both uniquely predicted subsequent increases in connectedness throughout treatment . - pubmed
Correlation of the preoperative MV - BMI ratio with preoperative Framingham risk score ( FRS ) and triglyceride / HDL - cholesterol ratio was investigated . - pubmed
RESULTS : Preoperative MV - BMI showed an inverse correlation with preoperative FRS and triglyceride / high - density lipoprotein - cholesterol ratio . - pubmed
BACKGROUND : Programmed cell death protein 1 / programmed death - ligand 1 ( PD-1 / PD - L1 ) blocking antibodies including cemiplimab have generated profound clinical activity across diverse cancer types . - pubmed
89Zr - REGN3504 immuno - PET accurately detected a significant reduction in splenic PD - L1 positive cells following systemic treatment with clodronate liposomes . - pubmed
CONCLUSION : This work supports the clinical translation of 89Zr - REGN3504 immuno - PET for the assessment of PD - L1 expression . - pubmed
Future clinical studies will aim to investigate the utility of 89Zr - REGN3504 immuno - PET for predicting and monitoring response to anti - PD-1 therapy . - pubmed
The rate of autoxidation of native oxymyoglobin to metmyoglobin has been examined over the pH range of 4.8 - -12.6 in 0.1 M buffer at 25 degrees C , and some 40 values of the observed first - order rate constant , kobs , are plotted against pH of the solution . - pubmed
The complicated pH - profile can be best explained by the ' acid - base catalyzed three states model ' , which reveals not only the catalytic role of hydrogen ions and hydroxyl ions , but also the involvement of two dissociation groups of myoglobin molecule in the autoxidation reaction . - pubmed
Antiproliferative effects of antisense oligonucleotides directed to the RNA of c - myc oncogene . - pubmed
Several groups have reported the use of antisense oligonucleotides to inhibit c - myc gene expression and study its biological role . - pubmed
To lower their concentration and stabilize the antisense effect against c - myc , oligonucleotides were covalently linked to poly ( L - lysine ) and administered in ternary complexes formed with heparin ( 100 micrograms / ml ) . - pubmed
A theoretical ( MM2 ) and experimental ( 1H and 13C NMR ) study of butaclamol hydrochloride in CDCl3 has been done in order to determine preferred conformations and internal molecular flexibility of this molecule . - pubmed
13C spin - lattice relaxation time measurements show that most of the molecule is rigid , although there is some degree of mobility in the seven - membered B ring , associated with rapid flipping of the bridging C8 and C9 carbons between two skewed conformations , which have previously been referred to as conformer A and conformer B ( Laus et al . - pubmed
Simultaneous injection of polymyxin B ( 10(-8 ) mol ( kg body weight)-1 ) , an inhibitor of protein kinase C , with TPA and CCK8 reversed the inhibitory effect of the phorbol ester on CCK8 - induced pancreatic juice flow , total protein output and amylase release . - pubmed
Prothrombinase assembly was studied on macroscopic planar bilayers consisting of 20 % dioleoyl - phosphatidylserine ( DOPS ) and 80 % dioleoyl - phosphatidylcholine ( DOPC ) . - pubmed
We performed a random assignment , double - blind , placebo - controlled study of nortriptyline ( NT ) in postpubertal 12- to 17 - year - olds with Research Diagnostic Criteria ( RDC ) and DSM - III major depressive disorder . - pubmed
The only observed biochemical change was a significant decrease in gamma - glutamyl cysteine synthetase the glutathione synthetic enzyme , in the carcinogen - induced preneoplastic and neoplastic line compared to control . - pubmed
Reduced glutathione ( GSH ) and non - protein sulfhydryl ( NPS ) levels were not reduced in the liver , kidney , or circulating red blood cells ( rbc ) by helenalin treatment . - pubmed
Combination of any of these agents , after three days , with helenalin afforded increases in P-388 cell GSH and NPS levels . - pubmed
Exons encoding the beta 1 , beta 2 and transmembrane domains of a B - L beta chain have been identified with 63 , 66 and 62 % similarity with the HLA - DQ beta sequence . - pubmed
Ornithine decarboxylase and diamine oxidase activities showed maximal increases at 24 h in the striatum of the lesioned side , whereas in the substantia nigra ornithine decarboxylase attained a very high value as early as 4 h after the operation and diamine oxidase activity peaked at 48 h. The enzyme activities returned toward the basal values at 168 h. On the intact side , ornithine decarboxylase showed a small increase starting at 4 h and diamine oxidase was enhanced at 48 h. These results indicate that the stimulation of biosynthetic and degradative enzymes of polyamine metabolism accompanied by marked and prolonged increases in putrescine may be essential events in the early phases of neuronal response to mechanical injury in the CNS . - pubmed
The two enzymes were separated by DEAE - cellulose chromatography and called kinases I and II . - pubmed
Adenosien 3 ' : 5 '- cyclic monophosphate stimulated the activity of both enzymes . - pubmed
In the presence of adenosine 3 ' : 5 '- cyclic monophosphate , protein kinase I dissociated into two subunits : a subunit binding adenosine 3 ' : 5 '- cyclic monophosphate , and a catalytic subunit . - pubmed
The mechanism of action of the cyclic nucleotide on kinase II appeared to be different from that of kinase I. - pubmed
In complete Krebs - Ringer bicarbonate buffer ( pH 7.4 ) the calcium - channel - blocking drugs verapamil ( 50 microM ) and flunarizine ( 50 microM ) had no effect on active renin release but increased inactive renin secretion . - pubmed
In Ca - free or Ca - depleted ( + EGTA ) buffers secretion of both active and inactive renin was increased . - pubmed
Neither verapamil nor flunarizine under these conditions had any additional effect on active renin secretion but inactive renin release was stimulated by verapamil and inhibited by flunarizine . - pubmed
Although having no effect in the presence of Ca ++ ions , the ionophore A23187 ( 17 microM ) selectively abolished the secretion of inactive renin in the absence of calcium . - pubmed
But there was a great difference in the apparent association rate constant ( kappa a ) between Domain II and Domain III at pH 8.0 ( the optimum pH of the enzymatic activity ) , and Domain III associated with TLE - Se about 10(5 ) times faster than Domain II . - pubmed
The pH dependency of kappa a for Domain III and TLE - Se was maximum around pH 8 , but in the case of Domain II and TLE - Se the maximum was around pH 4 . - pubmed
This suggested that some acidic amino acid residues had some influence upon the association of Domain II with TLE - Se . - pubmed
When we examined the interaction between TLE - Se and amidated Domain II in which most of the aspartic and glutamic acids were amidated for conversion into asparagine and glutamine with NH4Cl and carbodiimide , the pH dependence of kappa a greatly differed from that obtained for TLE - Se and native Domain II , and an almost constant kappa a value ( 10(3)-10(5 ) times higher than that of native Domain II ) was exhibited between pH 5 and 8 . - pubmed
At the same time , the dissociation constant at pH 8.0 became about 10(-2 ) times smaller , and thus the affinity between TLE - Se and amidated Domain II became stronger . - pubmed
We tested this hypothesis by analyzing pausing and non - pausing variants of both the pR ' promoter segment and a more active mutant version of pR ' ; we measured reporter gene expression and used KMnO4 footprinting to measure directly occupancy of the promoter and pause sites in growing cells . - pubmed
Activation of Ca2 + influx by transforming Ha - ras . - pubmed
The expression of transforming p21Ha - ras caused a significant increase in Mn2 + influx which was blocked by Cd2 + , La3 + , niguldipine and the Ca ( 2 +)- channel blocker SK&F96365 . - pubmed
In addition to the enhanced Mn2 + influx , transforming p21Ha - ras elicited an increased efflux of the K(+)-congener 86Rb + which was inhibitable by Ca ( 2 +)- channel blockers and charybdotoxin , a selective inhibitor of high and intermediate conductance Ca ( 2 +)- dependent K + channels . - pubmed
Charybdotoxin did not reduce the increase in Mn2 + influx by ras , demonstrating that the activation of Ca ( 2 +)- dependent K + channels was not required for the sustained Mn2 +/Ca2 + influx in the presence of transforming Ha - ras . - pubmed
In ras - expressing cells , the bradykinin - induced Mn2 + influx and charybdotoxin sensitive 86Rb + efflux were markedly potentiated . - pubmed
The increase in the inositol - 1,4,5 - trisphosphate and inositol-1,3,4,5 - tetrakisphosphate levels by ras is not sufficient to explain the elevated Mn2 + influx . - pubmed
The mitogenic response to an expression of transforming Ha - ras was inhibited by the Ca ( 2 +)- channel blockers not , however , by charybdotoxin . - pubmed
These data suggest the existence of an agonist - independent activation of a receptor- or second messenger - operated Ca2 + channel by transforming Ha - ras which is necessary for the mitogenic response to the activation of the oncogene . - pubmed
[ Preservation of PCNA in different fixatives and demonstration of PCNA as well as membrane antigen by double labelling technique ] . - pubmed
Four fixatives were used with two different fixation times in order to determine the influence of different fixatives on PCNA preservation . - pubmed
The result indicated that the aldehyde - base fixatives preserved PCNA excellently and the appropriate fixative time was 24 hours . - pubmed
The result of alcohol - base fixative on PCNA preservation seemed unsatisfactory and 10 % buffer formalin was the best one among the aldehyde - base fixatives for this preservation . - pubmed
Additionally , double - labelling technique for demonstrating PCNA as well as the membrane antigen in the same slide was established . - pubmed
Serum osteocalcin , serum total alkaline phosphatase , fasting urinary excretion of hydroxyproline and calcium , serum 25 - hydroxyvitamin D , and 1,25 dihydroxyvitamin D were assessed . - pubmed
Since the incidence of ER does not significantly differ between females and males , the observed sex differences regarding thyroid tumor incidence may reflect the higher estrogen serum content in females . - pubmed
RBI-257 : a highly potent dopamine D4 receptor - selective ligand . - pubmed
Dopamine D4 receptor affinity of iodo - isomers of RBI-257 ranked : para & gt ; meta & gt ; ortho . - pubmed
RBI-257 may be a useful probe or radioligand for brain dopamine D4 receptors . - pubmed
EPR detection of endogenous nitric oxide in postischemic heart using lipid and aqueous - soluble dithiocarbamate - iron complexes . - pubmed
Diethyldithiocarbamate ( DETC ) and Fe formed in ischemic - reperfused myocardium the lipophilic DETC2 - Fe - NO complex exhibiting an EPR signal ( g perpendicular = 2.04 and g parallel = 2.02 at 77 K ) with a triplet hyperfine structure at g perpendicular . - pubmed
Dithiocarbamate - Fe - NO complexes detected by both trapping agents were abolished by the .NO synthase inhibitor , NG - nitro - L - arginine methyl ester . - pubmed
For the lipoproteins , VLDL had the highest level of triglycerides followed by IDL , whereas the LDL particles had the highest levels of cholesterol esters compared to all other fractions . - pubmed
After separating the lipoprotein particles by size ( FPLC ) it was determined that both LDL and HDL carried approximately equal levels of cholesterol . - pubmed
The recombinant KIR2 has been crystallized in 9 - 10 % poly ( ethylene glycol ) methyl ether ( average Mr = 8000 ) , 50mM HEPES , 8 % ethylene glycol , 0.5 % octyl - beta - glucoside , pH 7.5 , at 294 K using the sitting - drop vapour - diffusion method . - pubmed
Disease activity was assessed by Ritchie articular index , erythrocyte sedimentation rate ( ESR ) and C - reactive protein ( CRP ) ; laboratory tests were done for deoxypyridinoline and bone alkaline phosphatase . - pubmed
The mean value for deoxypyridinoline and bone alkaline phosphatase on entry were 8.4 and 12.5 ; mean values of Ritchie articular index and ESR were initially 10.1 and 59 mm , respectively . - pubmed
At 3 months deoxypyridinoline levels had fallen to 6.2 , while bone alkaline phosphatase had risen to 18.8 . - pubmed
At 9 months there was significantly reduced deoxypyridinoline , to 5.5 ( p & lt ; 0.001 ) , while bone alkaline phosphatase had risen significantly to 30 ( p & lt ; 0.001 ) compared with pre - MTX assessment . - pubmed
Whereas cells cultured in GM - CSF alone were functionally immature , cells incubated with CD40L or LPS were mature BmDC , as evident by morphology , capacity to internalize Ag , migration into regional lymph nodes , IL-12 secretion , and alloantigen or peptide Ag presentation in vitro . - pubmed
The present data support the clinical use of mature , rather than immature , tumor Ag - pulsed dendritic cells as cancer vaccines and identifies CD40L as a potent stimulus to enhance their in vivo Ag - presenting capacity . - pubmed
The Erm methyltransferases confer resistance to macrolide , lincosamide and streptogramin B ( MLS ) antibiotics by methylation of a single adenosine base within bacterial 23 S ribosomal RNA . - pubmed
The core of the motif has the primary sequence 2056 - GGAHA-2060 , where H is any nucleotide except guanosine , and ErmE methylates at the adenosine in bold . - pubmed
Zener 's model of ferromagnetism , originally proposed for transition metals in 1950 , can explain T(C ) of Ga ( 1 -)( x ) Mn ( x ) As and that of its II - VI counterpart Zn ( 1 -)( x ) Mn ( x ) Te and is used to predict materials with T(C ) exceeding room temperature , an important step toward semiconductor electronics that use both charge and spin . - pubmed
The method is based on the extractive two - phase isobutoxycarbonyl ( isoBOC ) derivatization with subsequent solid - phase extraction ( SPE ) for the direct analysis by gas chromatography ( GC ) and gas chromatography - mass spectrometry ( GC - MS ) . - pubmed
The resulting isoBOC derivatives were then recovered by SPE using Chromosorb P in normal - phase partition mode , followed by direct GC and GC - MS analysis . - pubmed
protein - directed selectivity in reactions of hemoglobin with aryl trimesates . - pubmed
There are marked differences in the reaction patterns of these compounds with amino groups in hemoglobin compared to reactions with n - propylamine . - pubmed
The compounds that effectively modify the protein contain a carboxyl group ortho to the phenolic oxygen of the ester , while materials with meta or para carboxyl groups give little or no reaction . - pubmed
On the basis of these observations and the known reaction patterns of salicylates , it is clear that the environment in the protein controls the reaction , regardless of the inherent reactivity of the reagent . - pubmed
We propose that the carboxyl group positions the reagent critically within the protein . - pubmed
We postulate that the early drop - outs in the olanzapine - treated parkinsonian group may be attributable to a non - specific effect of the drug as a result of starting at too high a dose , and that the worsening of parkinsonism following prolonged treatment may have been caused by the drug 's blocking effect on striatal D2 receptors . - pubmed
These studies find that the elevation of corticosterone does not affect the expression of membrane receptors on macrophages and does not affect the activation of macrophages to produce cytokines . - pubmed
In contrast , elevated corticosterone levels correlate with enhanced T cell proliferation to both mitogens and superantigens . - pubmed
There were no significant differences in cyclic GMP and NOS analysis . - pubmed
Nitric oxide blocks fast , slow , and persistent Na + channels in C - type DRG neurons by S - nitrosylation . - pubmed
The NO scavenger hemoglobin abolished the effects of papa - NONOate on Na + currents , indicating that NO or NO - related species inhibit these Na + currents . - pubmed
Incubation of neurons with 8 - bromo cGMP , a membrane - permeable analogue of cGMP , and cG - PKI , an inhibitor of cGMP - dependent protein kinase , had no effect on papa - NONOate - mediated Na + current block , demonstrating that Na + current inhibition is independent of cGMP . - pubmed
Alkylation of free thiols with N - ethylmaleimide prevented the actions of papa - NONOate , suggesting that NO , or a related reactive nitrogen species , modifies sulfhydryl groups on Na + channels or a closely associated protein . - pubmed
These results demonstrate that inhibition of Na + currents is not due to the modulation of fast and slow sodium channel inactivation . - pubmed
Taken together , these results show that NO or NO - related products modify the sulfhydryl groups on Na + channels and inhibit Na + currents by blocking the channel conductance . - pubmed
Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarabine of acute myeloid leukemic cells . - pubmed
Inactivation of deoxycytidine kinase ( dCK ) plays an important role in AraC resistance in vitro . - pubmed
Because these dCK - spliced variants were only detectable in resistant AML , it was hypothesized that they might play a role in AraC resistance in vivo . - pubmed
However , a cell expressing alternatively spliced dCK forms that has lost wt dCK expression is resistant to the cytotoxic effects of AraC. - pubmed
CJ-13,981 and CJ-13,982 , new squalene synthase inhibitors . - pubmed
Two new squalene synthase ( SSase ) inhibitors , CJ-13,981 ( I ) and CJ-13,982 ( II ) , were isolated from the fermentation broth of an unidentified fungus CL 15036 . - pubmed
Based on FAB - MS and NMR analyses , the structures of I and II were determined to be 3 - hydroxy-3,4 - dicarboxy-15 - hexadecenoic acid and 3 - hydroxy-3,4 - dicarboxyhexadecanoic acid , respectively . - pubmed
To test the usefulness of RuBD as a probe for diffusive processes in calf thymus DNA , we compared the efficiencies of fluorescence resonance energy transfer ( FRET ) using three donors which display lifetimes near 5 ns for acridine orange ( AO ) , 22 ns for ethidium bromide ( EB ) and 92 ns for RuBD , with nile blue ( NB ) as the acceptor . - pubmed
The Forster distances for AO - NB , EB - NB and RuBD - NB donor - acceptor pairs were 42.3 , 52.3 , and 30.6 A , respectively . - pubmed
Decreased activity and enhanced nuclear export of CCAAT - enhancer - binding protein beta during inhibition of adipogenesis by ceramide . - pubmed
The addition of exogenous N - acetylsphingosine ( C2 - ceramide ) or increasing endogenous ceramide levels inhibited the expression of C / EBPalpha and PPARgamma , and blocked adipocyte development . - pubmed
C2 - ceramide did not decrease the cellular expression of C / EBPbeta , which is required for expression of C / EBPalpha and PPARgamma , but significantly blocked its transcriptional activity from a promoter construct after 24 h. The ceramide - induced decrease in the transcriptional activity of C / EBPbeta correlated with a strong decrease in its phosphorylation , DNA - binding ability and nuclear localization at 24 h. However , ceramide did not change the nuclear level of C / EBPbeta after a period of 4 or 16 h , suggesting that it was not affecting nuclear import . - pubmed
Leptomycin B is an inhibitor of CRM1 / exportin-1 , and reversed the ceramide - induced decrease in nuclear C / EBPbeta at 24 h. Taken together , these data support the hypothesis that ceramide may inhibit adipogenesis , at least in part , by enhancing dephosphorylation and premature nuclear export of C / EBPbeta at a time when its maximal transcriptional activity is required to drive adipogenesis . - pubmed
p42 / p44 MAPKs are intracellular targets of the CDK inhibitor purvalanol . - pubmed
Intensive screening of a combinatorial chemistry library of 2,6,9 - trisubstituted purines has led to the identification of purvalanol , one of the most potent and selective CDK inhibitors to date . - pubmed
p42 / p44 MAPK activity was visualized using an Elk - Gal4 luciferase reporter system and CDK1 activity was detected by the phosphonucleolin level . - pubmed
When cells were treated with purvalanol , p42 / p44 MAPK and CDK1 activities were inhibited in a dose - dependent manner . - pubmed
Furthermore , purvalanol inhibited the nuclear accumulation of p42 / p44 MAPK , an event dependent on the catalytic activity of these kinases . - pubmed
We conclude that the anti - proliferative properties of purvalanol are mediated by inhibition of both p42 / p44 MAPK and CDKs . - pubmed
Pretreatment of 4LHBMEC with glycosaminoglycan ( GAG)-degrading enzymes or sodium chlorate demonstrated that SDF-1 bound to both HSPGs and CS / DSPGs in a sulfation - dependent manner , as determined with an SDF-1 antibody recognizing the CXCR4 - binding site . - pubmed
[ Blood prothrombin during radiophosphorus and x - ray therapy of polycythemia and leukoses ] . - pubmed
Bilirubin levels in G6PD - deficient neonates were somewhat higher compared to G6PD - normal babies ( 18.8 + /- 2.4 mg / dl [ 321.5 + /- 41 micromol / l ] vs. 15.7 + /- 3.2 mg / dl [ 268.5 + /- 54.7 micromol / l ] ; P & lt ; 0.05 ) . - pubmed
Sodium dodecyl sulphate - polyacrylamide gel electrophoresis ( SDS - PAGE ) and immunoblotting demonstrated that the purified protein was recognized by both IL-1ra and IL-10 antibodies . - pubmed
The merozoite surface protein MSP-3beta is a member of a family of related merozoite surface proteins , all of which contain a central alanine - rich domain that is predicted to form a coiled - coil tertiary structure . - pubmed
In this first assessment of PvMSP-3 beta variation between populations , we discovered widespread and significant diversity , mostly within the alanine - rich central region . - pubmed
We observed frequent differences in PvMSP-3 beta gene size , caused by the insertion and/or deletion of several large sequence blocks , as well as numerous single nucleotide polymorphisms and smaller scale insertions and deletions . - pubmed
Lamellipodia production , or function , is defective and there is a selective reduction in the level and tyrosine phosphorylation of FAK , p130Cas , Crk , and Dock180 at nascent focal complexes . - pubmed
The high dairy diet resulted in decreases in total body fat ( 2.16 kg , p & lt ; 0.01 ) , trunk fat ( 1.03 kg , p & lt ; 0.01 ) , insulin ( 18.7 pM , p & lt ; 0.04 ) , and blood pressure ( 6.8 mm Hg systolic , p & lt ; 0.01 ; 4.25 mm Hg diastolic , p & lt ; 0.01 ) and an increase in lean mass ( 1.08 kg , p & lt ; 0.04 ) , whereas there were no significant changes in the low dairy group . - pubmed
Thyroid function tests revealed elevated serum thyroxine and triiodothyronine as well as low thyroid stimulating hormone concentrations , confirming the diagnosis of thyrotoxicosis . - pubmed
Because nitric oxide ( NO ) has been reported to play roles in the pathogenesis of acute lung injury , the present study explores the effects on VILI of NO derived from chronically overexpressed endothelial nitric oxide synthase ( eNOS ) . - pubmed
L - NAME abrogated the beneficial effect of eNOS overexpression . - pubmed
The mass and heat transfer dynamics of evaporating multicomponent alcohol / water droplets have been probed experimentally by examining changes in the near surface droplet composition and average droplet temperature using cavity - enhanced Raman scattering ( CERS ) and laser - induced fluorescence ( LIF ) . - pubmed
Since both events might be linked to the inhibition of K + channels , we studied whether in vivo application of minoxidil , a stimulator of ATP - gated K channels ( K+ ATP channel activator ) prevents both effects . - pubmed
This twelve - week randomized , double - masked , placebo - controlled study assessed the efficacy and tolerability of the specific and potent oral dipeptidyl peptidase-4 inhibitor , vildagliptin ( 25 mg , bid , n=70 ) VS . - pubmed
The between - group differences in corrected insulin response at peak glucose and mean prandial C - peptide were + 0.06 + /- 0.02 ( p=0.0258 ) and + 0.10 + /- 0.03 nmol / l ( p=0.0031 ) , respectively . - pubmed
We have previously shown that cysteine - affecting mutations could lead to partial endoplasmic reticulum ( ER ) retention . - pubmed
Treatment of primary oviductal cells with 10 pg / ml 17beta - estradiol or 10 ng / ml progesterone resulted in a higher expression of COX-2 and cPLA ( 2 ) alpha , but not of the other enzymes . - pubmed
The dissimilatory reduction of 1mmol / L AQDS can couple to the production of enough ATP to support cell growth about 60 generations ; The oxidization of electron donors was coupled to the reduction of humus , as reduced humus increased corresponding with increasing of electron donor ; The typical inhibitors such as Cu2 + which inhibited Fe - S center , Stigmatellin which was methyl - naphthoquinone model , Dicumarol which inhibited oxidized methyl - naphthoquinone transform to reduced one , Metyrapone which was specific inhibitor for P450 enzyme blocked the humus respiration seriously . - pubmed
Is the effect of testosterone on body composition modulated by the androgen receptor gene CAG repeat polymorphism in elderly men ? - pubmed
OBJECTIVE : The androgen receptor ( AR ) gene contains a CAG repeat polymorphism coding for a polyglutamine chain , the length of which is inversely correlated with AR transcriptional activity . - pubmed
Here , we identify a highly conserved 9 amino acid motif in the ligand binding domains ( E domains ) of human / mouse ER alpha and ER beta , progesterone receptors A and B , and the androgen receptor . - pubmed
Mutation of the phenylalanine or tyrosine at position-2 , cysteine at position 0 , and hydrophobic isoleucine / leucine or leucine / leucine combinations at positions +5/6 , relative to cysteine , significantly reduced membrane localization , MAP and PI 3 - kinase activation , thymidine incorporation into DNA , and cell viability , stimulated by specific SR ligands . - pubmed
The localization sequence mediated palmitoylation of each SR , which facilitated caveolin-1 association , subsequent membrane localization , and steroid signaling . - pubmed
RESULTS : ( 1 ) Compared with that at 0 min , the mean fluorescence intensity of JC-1 within the spermatozoal body and the percentage of orange - red colored spermatozoa in the experiment group were increased significantly at 5 and 15 min respectively after uPA incubation ( P & lt ; 0.05 ) . - pubmed
[ Sequential acupuncture method for treatment of apoplexy hemiplegia and its effects on plasma ET-1 and NO levels ] . - pubmed
After treatment for 3 months , their clinical therapeutic effects were observed and plasma endothelin-1 ( ET-1 ) and nitric oxide ( NO ) levels were detected . - pubmed
RESULTS : The treatment group in the improvement of the nerve function - deficiency , total life ability and clinical overall effect , and the course of recovery , decreasing of ET-1 level and increasing of NO level was superior to the control group ( P & lt ; 0.05 , P & lt ; 0.01 ) , the shorter the course , the better the therapeutic effect . - pubmed
CONCLUSION : The sequential acupuncture method has an obvious therapeutic effect on apoplexy hemiplegia , and it can decrease plasma ET-1 level and increase NO level . - pubmed
Mutation of the nuclear factor - kappaB ( NF - kappaB ) binding site significantly impaired both DON- and LPS - induced luciferase expression . - pubmed
In contrast , mutating the activator protein-1 binding site resulted in significantly increased DON- and LPS - induced luciferase expression . - pubmed
CCAAT / enhancer - binding protein beta , octamer-1 , or NF - kappaB repressing factor binding site mutations did not affect DON - induced luciferase activity . - pubmed
Consistent with reporter studies , the NF - kappaB inhibitor caffeic acid phenethyl ester completely ablated both DON - induced IL-8 mRNA and protein expression . - pubmed
When NF - kappaB subunit binding to a specific IL-8 promoter probe was evaluated by enzyme - linked immunosorbent assay ( ELISA ) , DON was observed to increase p65 binding by 21 - fold , have no effect on p50 binding and decrease p52 binding . - pubmed
Taken together , these data suggest that DON - induced IL-8 expression is likely to be mediated at the transcriptional level by NF - kappaB , specifically p65 , but does not appear to involve mRNA stabilization . - pubmed
Cardiac microdialysis technique demonstrated that topical perfusion of acetylcholine increased dialysate TIMP-1 protein level , which was suppressed by coperfusion of atropine . - pubmed
Immunohistochemistry demonstrated a strong expression of TIMP-1 protein in cardiomyocytes around the dialysis probe used to perfuse acetylcholine . - pubmed
Inhibition of nitric oxide production by the carbazole compound LCY-2 - CHO via blockade of activator protein-1 and CCAAT / enhancer - binding protein activation in microglia . - pubmed
LCY-2 - CHO did not block DNA - binding activity of nuclear factor - kappaB ( NF - kappaB ) or cyclic AMP response element - binding protein ( CREB ) , but abolished that of activator protein-1 ( AP-1 ) , CCAAT / enhancer - binding protein ( C / EBP ) and nuclear factor IL6 ( NF - IL6 ) . - pubmed
LCY-2 - CHO attenuated the nuclear levels of c - Jun and C / EBPbeta , but not those of p65 , p50 , C / EBPdelta , signal transducer and activator of transcription-1 ( STAT-1 ) or the nuclear expression of IFN regulatory factor-1 ( IRF-1 ) . - pubmed
Taken together , these results suggest that LCY-2 - CHO inhibits NO production in microglia through the blockade of AP-1 and C / EBP activation . - pubmed
Our multiple regression model for DNA tail moment showed that age , high pesticide exposure , low pesticide exposure , GSTP1 Ile - Ile , and XRCC1 399 Arg - Arg genotype were associated with increased DNA tail moment ( DNA damage ) . - pubmed
Further analysis of interaction between GSTP1 and XRCC1 genes that increase susceptibility revealed a significant difference in DNA tail moment for high pesticide - exposed subjects carrying both GSTP1 Ile - Ile with XRCC1 399 Arg - Arg genotypes ( 2.49+/-0.09 microm / cell ; P=0.004 ) , compared to those carrying GSTP1 Ile - Val / Val - Val with XRCC1 399 Arg - Gln / Gln - Gln genotypes ( 1.98+/-0.15 microm / cell ) . - pubmed
Pectolinarigenin strongly inhibited COX-2 - mediated PGE2 and 5 - LOX - mediated LT production at & gt;1 microM , indicating that it is a dual inhibitor of COX-2 / 5 - LOX . - pubmed
However , pectolinarigenin did not affect COX-2 expression or nuclear transcription factor ( NF - kappaB ) activation . - pubmed
Endothelial barrier protection by FTY720 under hyperglycemic condition : involvement of focal adhesion kinase , small GTPases , and adherens junction proteins . - pubmed
The phosphorylated form of FTY720 binds to S1P receptors to exert S1P - like biological effects , suggesting endothelial barrier promotion by FTY720 . - pubmed
Similar to S1P , site - specific FAK tyrosine phosphorylation analysis revealed FTY720 - induced FAK [ Y576 ] phosphorylation without phosphorylation of FAK [ Y397 / Y925 ] . - pubmed
Furthermore , FTY720 conditioned the phosphorylation profile of FAK [ Y397 / Y576 / Y925 ] in hyperglycemic medium to the same pattern observed in normoglycemic medium . - pubmed
FTY720 challenge resulted in small GTPase Rac activation under hyperglycemic conditions , whereas increased Rho activity in hyperglycemic medium was restored to the basal level . - pubmed
Rac protein depletion by small interfering RNA ( siRNA ) technique completely abolished FTY720 - induced FAK [ Y576 ] phosphorylation . - pubmed
These findings strongly suggest the barrier protective effect of FTY720 on HMVEC monolayers in hyperglycemic medium via S1P signaling , further implying the possibility of FTY720 as a therapeutic agent of diabetic vascular disorder . - pubmed
After release from their precursor kininogens , kinins or their C - terminal des - Arg metabolites activate two distinct G protein - coupled receptors ( GPCR ) , called B2 ( B2R ) or B1 ( B1R ) . - pubmed
Both receptors can couple to Galphai and Galphaq families of G proteins to release mediators such as nitric oxide ( NO ) , arachidonic acid , prostaglandins , leukotrienes and endothelium - derived hyperpolarizing factor and can induce the release of other inflammatory agents . - pubmed
NrdH is one of the intracellular redoxins exist in Mycobacterium and other microorganisms lacking the common glutathione system . - pubmed
NrdH seems a chimeric protein of glutaredoxin amino acid sequence and thioredoxin activity . - pubmed
It serves as a hydrogen donor for ribonucleotide reductase . - pubmed
Results led to a call for more modest treatment goals ( initiation of insulin at a blood glucose & gt;180 mg / dl with a goal of approximately 150 mg / dl ) . - pubmed
To this end , we treated bone marrow - derived MSC with estradiol and progesterone , bone morphogenetic protein 2 ( BMP2 ) , and activators of the protein kinase A ( PKA ) pathway and investigated specific markers of hESF differentiation ( decidualization ) . - pubmed
Furthermore , we investigated the transcriptome of these cells in response to cAMP and compared this to the transcriptome of hESF decidualized in response to activation of the PKA pathway . - pubmed
Bioactive glasses ( BG ) which contain strontium have the potential to combine the known bone regenerative properties of BG with the anabolic and anti - catabolic effects of strontium cations . - pubmed
Here we created a BG series ( SiO ( 2 )- P ( 2 ) O ( 5 )- Na ( 2 ) O - CaO ) in which 0 - 100 % of the calcium was substituted by strontium and tested their effects on osteoblasts and osteoclasts in vitro . - pubmed
They also inhibit osteoclast activity by both reducing tartrate resistant acid phosphatase activity and inhibiting resorption of calcium phosphate films in a dose - dependent manner . - pubmed
Additionally , osteoblasts cultured in contact with BG show increased proliferation and alkaline phosphatase activity with increasing strontium substitution , while osteoclasts adopt typical resorption morphologies . - pubmed
These effects , when combined with the advantages of BG such as controlled ion release and delivery versatility , may make strontium - substituted BG an effective biomaterial choice for a range of bone regeneration therapies . - pubmed
When taxol was added , treated cells arrested indicating that microtubule turnover was necessary for kinetochore fragment separation . - pubmed
BACKGROUND / AIM : 9 -[ 2 -( phosphonomethoxy ) ethyl ] guanine ( PMEG ) is a guanine acyclic nucleotide analog whose targeted prodrugs are being investigated for chemotherapy of lymphomas . - pubmed
The objective of this study was to determine the requirements for caspase and CD95 / Fas activation in PMEG - induced apoptosis . - pubmed
Additionally , the influence of PMEG on cell cycle regulatory proteins was explored . - pubmed
Indole and aliphatic glucosinolates accumulated in response to K - deficiency in a manner that was respectively dependent or independent on signaling through Coronatine - Insensitive 1 ( COI1 ) . - pubmed
Amrubicin at a lower - dose with routine prophylactic use of granulocyte - colony stimulating factor for relapsed small - cell lung cancer . - pubmed
To overcome this clinical problem , we investigated whether 35 mg / m(2 ) of AMR administration with routine prophylactic use of granulocyte - colony stimulating factor ( G - CSF ) can reduce myelosuppression , while maintaining efficacy . - pubmed
CO reduced acute tubular necrosis , apoptosis , tissue factor expression and P - selectin expression and enhanced proliferative repair as measured by phosphorylation of retinol binding protein and histone H3 . - pubmed
Gene microarray analyses with confirmatory PCR of biopsy specimens showed that CO blocked proinflammatory gene expression of MCP-1 and heat shock proteins . - pubmed
Complete ophthalmic examination including visual acuity , fundus biomicroscopy , fundus photography , fluorescein angiography , and spectral domain optical coherence tomography ( Spectralis SD - OCT ; Heidelberg Engineering , Heidelberg , Germany ) was performed at baseline and follow - up ( 1 week , 1 month , and 3 months ) , and tolerability of the implant was assessed . - pubmed
During protein synthesis , translation elongation factor Tu ( Ef - Tu ) is responsible for the selection and binding of the cognate aminoacyl - tRNA to the acceptor site on the ribosome . - pubmed
The activity of Ef - Tu is dependent on its interaction with GTP . - pubmed
tuberculosis Ef - Tu ( MtbEf - Tu ) by PknB reduced its interaction with GTP , suggesting a concomitant reduction in the level of protein synthesis . - pubmed
MtbEf - Tu was found to be phosphorylated by PknB on multiple sites , including Thr118 , which is required for optimal activity of the protein . - pubmed
We found that kirromycin , an Ef - Tu - specific antibiotic , had a significant effect on the nucleotide binding of unphosphorylated MtbEf - Tu but not on the phosphorylated protein . - pubmed
Our results show that the modulation of the MtbEf - Tu - GTP interaction by phosphorylation can have an impact on cellular protein synthesis and growth . - pubmed
15 ( S )- HETE at concentration 0.1 muM stimulated proliferation of PASMCs and induced ERK 1 / ERK 2 phosphorylation but had no effect on p38 kinase expression as assessed by Western blotting . - pubmed
15 ( S )- HETE - stimulated PASMC proliferation was blocked by the MEK inhibitors PD-98059 . - pubmed
Hypoxia ( 3 % O ( 2 ))-stimulated PASMC proliferation was blocked by U0126 , a MEK inhibitor , as well as by NDGA and CDC , inhibitors of 15 - LOX , but not by the p38 MAPK inhibitor SB-202190 . - pubmed
Glucagon - like peptide-1 ( GLP-1 ) and glucose - dependent insulinotropic polypeptide ( GIP ) are incretins produced in the intestine that play a central role in glucose metabolism and insulin secretion . - pubmed
Inhibition of chylomicron formation with L-81 reduced GLP-1 secretion into lymph compared to Liposyn stimulation alone ( P = 0.034 ) . - pubmed
The effect of L-81 relative to Liposyn alone had an even greater effect on GIP secretion , which was completely abolished ( P = 0.004 ) . - pubmed
These findings of a dramatic effect of L-81 on lymph levels of GLP-1 and GIP support a strong link between intestinal lipid absorption and incretin secretion . - pubmed
The relative difference in the effect of L-81 on the two incretins provides further support that nutrient - stimulation of GIP and GLP-1 is via distinct mechanisms . - pubmed
Lipopolysaccharide / adenosine triphosphate - mediated signal transduction in the regulation of NLRP3 protein expression and caspase-1 - mediated interleukin-1beta secretion . - pubmed
OBJECTIVE : Reactive oxygen species ( ROS ) plays a critical role in the regulation of NLRP3 inflammasome activation . - pubmed
ROS was scavenged by N - acetyl cysteine ; NADPH oxidase , the major source of ROS , was inhibited by diphenyliodonium , apocynin or gp91 - phox siRNA transfection ; and protein kinase was inhibited by its specific inhibitor . - pubmed
RESULTS : LPS - induced NLRP3 protein expression was regulated through the NADPH oxidase / ROS / NF - kappaB - dependent , JAK2 / PI3 - kinase / AKT / NF - kappaB - dependent , and MAPK - dependent pathways , while ATP - induced caspase-1 activation was regulated through the NADPH oxidase / ROS - dependent pathway . - pubmed
CONCLUSIONS : These results demonstrate that ROS regulates not only the priming stage , but also the activation stage , of NLRP3 inflammasome activation in LPS + ATP - activated macrophages . - pubmed
Mutagenesis of the CPXV203 / MHCI interface identified the importance of two CPXV203 His residues that confer low pH stabilization of the complex and are critical to ER retrieval of MHCI . - pubmed
The first step of the NETotic cascade is stimulation of one or several receptors followed by activation of the Raf / MEK / ERK pathway that culminates in the assembly of the multimeric NADPH oxidase complex and the production of reactive oxygen species ( ROS ) . - pubmed
Of these 25 , a single protein , hydroxysteroid dehydrogenase like 2 , showed decreased expression in both males and females following arsenic exposure . - pubmed
We found that the cells expressing BmAsns protein were capable of influencing the formation of autophagic vacuoles stimulated by amino acid deprivation . - pubmed
Altogether , we concluded that the up - regulated expression of BmASNS would be able to protect cells from impairment induced by amino acid deprivation , which in turn facilitates cell growth and survival . - pubmed
Phosphorylation of the activation loop tyrosine 823 in c - Kit is crucial for cell survival and proliferation . - pubmed
Regulation of the catalytic activity of several kinases is known to be governed by phosphorylation of tyrosine residues in the activation loop of the kinase domain . - pubmed
However , in the case of c - Kit phosphorylation of Tyr-823 has been demonstrated to be a late event that is not required for kinase activation . - pubmed
By using a tyrosine - to - phenylalanine mutant of tyrosine 823 , we investigated the impact of Tyr-823 on c - Kit signaling . - pubmed
We demonstrate here that Tyr-823 is crucial for cell survival and proliferation and that mutation of Tyr-823 to phenylalanine leads to decreased sustained phosphorylation and ubiquitination of c - Kit as compared with the wild - type receptor . - pubmed
Thus , we propose that activation loop tyrosine 823 is crucial for activation of both the MAPK and PI3 K pathways and that its disruption leads to a destabilization of the c - Kit receptor and decreased survival of cells . - pubmed
Structure activity relationship studies of 17 - cyclopropylmethyl-3,14beta - dihydroxy-4,5alpha - epoxy-6alpha -( isoquinoline-3 '- carboxamido ) morphinan ( NAQ ) analogues as potent opioid receptor ligands : preliminary results on the role of electronic characteristics for affinity and function . - pubmed
17 - Cyclopropylmethyl-3,14beta - dihydroxy-4,5alpha - epoxy-6alpha -( isoquinoline-3 '- carboxamido ) morphinan ( NAQ ) was previously designed following the ' message - address ' concept and was identified as a potent and highly selective mu opioid receptor ( MOR ) ligand based on its pharmacological profile . - pubmed
For the new ligands synthesized as NAQ analogues , their binding assay results showed that a longer spacer and a saturated ring system of the side chain were unfavorable for their MOR selectivity over the kappa and delta opioid receptors . - pubmed
In contrast , substitutions with different electronic properties at either 1'- or 4'-position of the isoquinoline ring of the side chain were generally acceptable for reasonable MOR selectivity . - pubmed
The majority of NAQ analogues retained low efficacy at the MOR compared to NAQ in the ( 35 ) S - GTP [ gammaS ] binding assays while electron - withdrawing groups at 1'-position of the isoquinoline ring induced higher MOR stimulation than electron - donating groups did . - pubmed
In summary , the electronic characteristics of substituents at 1'- or 4'-position of the isoquinoline ring in NAQ seem to be critical and need to be further tuned up to achieve higher MOR selectivity and lower MOR stimulation . - pubmed
OBJECTIVE : This study was undertaken in the belief that the atypical antipsychotic drug quetiapine could prevent apoptosis in the penumbra region following ischemia , taking into account findings that show 5 - hydroxytryptamine-2 receptor blockers can prevent apoptosis . - pubmed
The D - I group was administered quetiapine following ischemia and sacrificed on the 1st day while the D - II group was administered quetiapine every day following the ischemia and sacrificed on the 3rd day . - pubmed
ROC analysis reported Met levels and DNA MET % , respectively , with a slight and moderate discriminative power . - pubmed
The degree to which PBA was bonded to chitosan was related to the 4 - FPBA load used in the reaction ( termed F1 through to F6 with increasing 4 - FPBA load ) . - pubmed
Topographic analysis by SEM revealed that PBA - chitosan conjugates F1 , F2 and F3 have porous matrices and their sensitivity to glucose adsorption was directly proportional to the degree of PBA substitution onto chitosan . - pubmed
Conversely , conjugates F4 , F5 and F6 appeared crystalline under SEM and glucose adsorption sensitivity decreased in proportion to amount of PBA bonded to chitosan . - pubmed
Nephrotoxicity was induced in Groups I - IV on the 20th day by the administration of a single dose of cisplatin solution ( 7 mg / kg body weight , i.p . ) - pubmed
TA not only prevented the development of retinal vascular lesions but also inhibited fluorescein leakage from established vascular lesions . - pubmed
This targeting to transcribed sequences requires SETD2 - mediated methylation of lysine 36 on histone H3 and a functional PWWP domain of DNMT3B. - pubmed
RESULTS : The maximum concentration of alpha - terpineol ( 833.93 mg L(-1 ) ) was obtained when the pre - culture medium was in medium log - phase by adding 840 mg L(-1 ) substrate dissolved in ethanol and cultivation was performed at 24 C , 150 rpm , and pH 6.0 for 12 h. Addition of small amounts of ( R )-(+)- limonene ( 84 mg L(-1 ) ) at the start of fungal log - phase growth yielded a 1.5 - fold yield of alpha - terpineol , indicating that the enzyme was inducible . - pubmed
Although basal insulin secretion was remarkably high in the isolated pancreatic islets , the responses to both glucose stimulation and the incretin GLP-1 were retained . - pubmed
In the pancreatic islets , the insulin secretory response to glucose stimulation was maintained but the response to the incretin was diminished . - pubmed
Ammonium transporters ( AMTs ) are plasma membrane proteins that exclusively transport ammonium / ammonia . - pubmed
Expression analyses showed that 14 AMT genes were vegetative organs expressed ; AMT1 ; 1 / 1 ; 3 / 1 ; 6 / 3 ; 2 and AMT1 ; 1 / 1 ; 2 / 2 ; 2 / 3 ; 1 had high transcript accumulation level in the leaves and roots , respectively and strongly changes under the nitrogen - dependent experiments . - pubmed
The results imply the functional roles of AMT genes in ammonium absorption in poplar . - pubmed
Re : Ion Channel TRPM8 Promotes Hypoxic Growth of Prostate Cancer Cells via an O2 - Independent and RACK1 - Mediated Mechanism of HIF-1alpha Stabilization . - pubmed
In this study , we analyze the effects of the dual kinase inhibitor PI-103 , which blocks PI3 K and mTOR activity , on three matched pairs of GBM stem cells / differentiated cells . - pubmed
While blocking PI3K - mediated signaling concurrently with application of chemotherapy does not appear to be a valid treatment option , pharmacological inhibitors , such as PI-103 , nevertheless have an important place in future therapeutic approaches . - pubmed
The responses to lenalidomide seem to be inversely correlated to the pre - treatment EPO level . - pubmed
This system utilized one - pot nanocomposite entrapping Fe3O4 magnetic nanoparticles ( MNPs ) as peroxidase mimetics and glucose oxidase ( GOx)/cholesterol oxidase ( ChOx ) in mesoporous silica to detect glucose and cholesterol in blood samples . - pubmed
The sensing mechanism involves the generation of H2O2 by the catalytic action of an immobilized oxidase on the target molecules in the sample . - pubmed
The regulatory role of interferon - gamma producing gamma delta T cells via the suppression of T helper 17 cell activity in bleomycin - induced pulmonary fibrosis . - pubmed
Our aim was to evaluate the neuroprotective effects of guanosine against oxidative damage in hippocampal slices subjected to an in vitro ischemia model , the oxygen / glucose deprivation ( OGD ) protocol . - pubmed
We also assessed the participation of nitric oxide synthase ( NOS ) enzymes activity on the neuroprotection promoted by guanosine . - pubmed
Here , we showed that guanosine prevented the increase in ROS , nitric oxide , and peroxynitrite production induced by OGD . - pubmed
Moreover , guanosine prevented the loss of mitochondrial membrane potential in hippocampal slices subjected to OGD . - pubmed
The protective effects of guanosine were mimicked by inhibition of neuronal NOS , but not of inducible NOS . - pubmed
The neuroprotective effect of guanosine may involve activation of cellular mechanisms that prevent the increase in nitric oxide production , possibly via neuronal NOS . - pubmed
In further experiments , vitamin B12 - dependent methylmalonyl - CoA mutase and methionine synthase activities were detected in cell homogenates . - pubmed
In particular , methionine synthase activity was significantly increased following the addition of vitamin B12 to the medium . - pubmed
It remains unclear , however , whether ethanol also increases NAc DA transmission following operant oral self - administration ( SA ) . - pubmed
As a follow up to these studies , we recently investigated the changes in NAc shell and core DA transmission associated to oral SA of a 10 % ethanol solution . - pubmed
For the purpose of comparison with literature studies utilizing sucrose + ethanol solutions , we also investigated the changes in dialysate DA associated to SA of 20 % sucrose and 10 % ethanol + 20 % sucrose solutions . - pubmed
Conversely , under sucrose SA and extinction DA increased exclusively in the shell . - pubmed
These observations provide unequivocal evidence that oral SA of 10 % ethanol increases dialysate DA in the NAc , and also suggest that stimuli conditioned to ethanol exposure contribute to the increase of dialysate DA observed in the NAc following ethanol SA . - pubmed
Comparison between the pattern of DA changes detected in the NAc subdivisions under sucrose and ethanol SA likewise suggests that the NAc shell and core DA play different roles in sucrose as compared to ethanol reinforcement . - pubmed
Lecithin : Cholesterol Acyltransferase Activation by Sulfhydryl - Reactive Small Molecules : Role of Cysteine-31 . - pubmed
Lecithin : cholesterol acyltransferase ( LCAT ) catalyzes plasma cholesteryl ester formation and is defective in familial lecithin : cholesterol acyltransferase deficiency ( FLD ) , an autosomal recessive disorder characterized by low high - density lipoprotein , anemia , and renal disease . - pubmed
This study aimed to investigate the mechanism by which compound A [ 3 -( 5 -( ethylthio )- 1,3,4 - thiadiazol-2 - ylthio ) pyrazine-2 - carbonitrile ] , a small heterocyclic amine , activates LCAT . - pubmed
The site - directed mutation LCAT - Cys31Gly prevented activation by compound A. - pubmed
Substitution of Cys31 with charged residues ( Glu , Arg , and Lys ) decreased LCAT activity , whereas bulky hydrophobic groups ( Trp , Leu , Phe , and Met ) increased activity up to 3 - fold ( P & lt ; 0.005 ) . - pubmed
Mass spectrometry of a tryptic digestion of LCAT incubated with compound A revealed a +103.017 m / z adduct on Cys31 , consistent with the addition of a single hydrophobic cyanopyrazine ring . - pubmed
Finally , sulfhydryl - reactive beta - lactams were developed as a new class of LCAT activators . - pubmed
In conclusion , compound A activates LCAT , including some FLD mutations , by forming a hydrophobic adduct with Cys31 , thus providing a mechanistic rationale for the design of future LCAT activators . - pubmed
Mutation in the conserved cysteine residue in the NEL domain of RipAW completely abolished the E3 ubiquitin ligase activity in vitro and the ability to suppress PTI responses in plant leaves . - pubmed
Unlike other members of the IpaH family of effectors , RipAW and RipAR had no leucine - rich repeat motifs in their amino acid sequences . - pubmed
BACKGROUND : Proprotein convertase subtilisin / kexin type 9 inhibitors , Praluent ( alirocumab [ ALI ] ) and Repatha ( evolocumab [ EVO ] ) have been approved as adjuncts to the standard - of - care maximal - tolerated dose ( MTD ) of low - density lipoprotein cholesterol ( LDLC)-lowering therapy ( LLT ) , statin therapy , in heterozygous ( HeFH ) ( ALI or EVO ) or homozygous ( EVO ) familial hypercholesterolemia , or clinical atherosclerotic cardiovascular disease ( CVD ) where LDLC lowering is insufficient ( both ) . - pubmed
SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0.86 g L-1 h-1 and the yield of 0.40 g g-1 sugar . - pubmed
The final skeleton obtained depends on the nature of both the R2 group of the allene and the R3 group of the allylic residue . - pubmed
Cholinergic excitation of corticopontine neurons involves at least three ionic mechanisms : suppression of KV 7 currents , activation of the calcium - dependent non - specific cation conductance underlying afterdepolarizations , and activation of what appears to be a calcium - sensitive but calcium - permeable non - specific cation conductance . - pubmed
Cholinergic excitation of CPn neurons involved at least three distinct ionic mechanisms : suppression of KV 7 channels ( the ' M - current ' ) , activation of the calcium - dependent non - specific cation conductance underlying afterdepolarizations , and activation of what appears to be a calcium - sensitive but calcium - permeable non - specific cation conductance . - pubmed
[ Construction and characterization of hemolysin S gene mutant strain producing hyaluronic acid ] . - pubmed
The yield of HA , HA molecular weight and virulence factors such as streptolysin Hylc , hyaluronate lyase , glyceraldehyde-3 - phosphate dehydrogenase and cell surface proteins were determined by spectrophotometer and SDS - PAGE . - pubmed
Berberine Attenuates Macrophages Infiltration in Intracranial Aneurysms Potentially Through FAK / Grp78 / UPR Axis . - pubmed
Immunostaining data showed that BBR inhibited CD68 + macrophages accumulation in IAs tissues and suppressed FAK phosphorylation . - pubmed
Conclusion : BBR prevents IAs formation potentially through inhibiting FAK phosphorylation and inactivating UPR pathway in macrophages , which causes less macrophage infiltration and reduced proinflammatory cytokine release . - pubmed
The biological indicators of Qi deficiency related were blood lipids , ECG , blood rheology , inflammatory reaction , NO , ET and NF - kappaB signalling pathway . - pubmed
Systolic ( SBP ) and diastolic ( DBP ) blood pressures were measured using a standard mercury sphygmomanometer . - pubmed
Fasting blood glucose ( FBG ) , lipid profile , uric acid and blood urea nitrogen ( BUN ) , and hs - CRP were measured using routine methods . - pubmed
lucidum alcohol extracts could improve learning and memory function , ameliorate neuronal apoptosis and brain atrophy , and down - regulate the expression of the AD intracellular marker , Abeta1 - 42 . - pubmed
We found a subset of MDR inhibitors also induced IEC delamination , implicating MDR efflux pumps as cellular targets underlying Glafenine - induced enteropathy . - pubmed
Optimization of extraction conditions by a central composite experimental design revealed that the dilution of the alcohol content of 5 mL of Baijiu to 10 % , followed by the addition of 1.5 g of NaCl and subsequent SPME Arrow extraction with DVB / CAR / PDMS fiber coating over 45 min at 45 C was the most suitable . - pubmed
Expression of SPH kinases ( SPHK ) 1 and 2 , which splice SPH into S1P , was also inhibited by MAT treatment . - pubmed
In vitro studies showed a direct inhibitory effect of MAT on S1P1 expression of activated astrocytes , suggesting that MAT could function as an S1PRs antagonist . - pubmed
Moreover , MAT upregulated the expression of plasma gelsolin , which combines with S1P to reduce its concentration . - pubmed
Interestingly , FMT from SHR - losartan to SHR reduced BP , improved the aortic endothelium - dependent relaxation to acetylcholine and reduced NADPH oxidase activity . - pubmed
Cetirizine is a second - generation antihistamine , derived from the metabolism of hydroxyzine , highly specific for the H1 receptors , and with marked antiallergic properties . - pubmed
HLA - C * 06:284 differs from HLA - C * 06:02:01:02 by two single nucleotide substitutions in codon 24 ( Ser > Thr ) . - pubmed
The branched - chain amino acids ( BCAA ) play an important role in muscle energy metabolism , and Kruppel - like factor 15 ( KLF15 ) is an essential regulator of BCAA metabolism in muscle under nutritional deficiency . - pubmed
It can significantly increase the proportion of G2 / M phase ( up to 3.2 - fold enhancement ) to reactivate cisplatin via high level of G2 / M arrest induced by valproate . - pubmed
Meanwhile , the intracellular ROS - P53 crosstalk can be upregulated by diallyl disulfide ( up to 8 - fold increase of ROS ) and valproate ( up to 18 - fold increase of P53 ) to enhance early apoptosis . - pubmed
The synchronization of enhanced G2 / M arrest and ROS - P53 crosstalk devotes to reverse the cisplatin resistance with a high level of resistance reversion index ( 50.22 ) . - pubmed
Determinants of Maternal Renin - Angiotensin - Aldosterone - System Activation in Early Pregnancy : Insights From Two Cohorts . - pubmed
It may thus contribute to the renin - angiotensin - aldosterone - system ( RAAS ) activation that is required for maternal adaptation in pregnancy . - pubmed
METHODS : Quantification of maternal prorenin , renin and aldosterone blood levels at 5 , 9 and 11 weeks of gestation . - pubmed
RESULTS : Prorenin and renin were lower in the absence of a CL at all - time points when compared to 1 CL , while prorenin , renin and aldosterone were higher in the presence of > 1 CL versus 1 CL ( P<0.05 ) . - pubmed
Ovarian stimulation with menopausal gonadotropin resulted in higher prorenin , renin and aldosterone concentrations during late first trimester than recombinant follicle - stimulating hormone ( P<0.05 ) . - pubmed
Prorenin , and to a lesser degree renin , correlated positively with serum progesterone and relaxin , but not serum estradiol . - pubmed
AIM AND OBJECTIVE : Production of light olefins from methanol was studied over SAPO-34 molecular sieves exploring the effect of mono and dual templates . - pubmed
A novel homozygous variant in REN in a family presenting with classic features of disorders involving the renin - angiotensin pathway , without renal tubular dysgenesis . - pubmed
Autosomal recessively inherited pathogenic variants in genes associated with the renin - angiotensin - aldosterone system ( RAAS ) result in early onset oligohydramnios and clinical features of the Potter sequence , typically in association with proximal renal tubules dysgenesis . - pubmed
Carbon capture and storage ( CCS ) technologies in shale reservoirs have attracted increasing interest in recent years . - pubmed
In this study , the characteristics of the CO2 adsorption in shale and its relationship with the shale nano - structure were studied , which may be helpful for understanding CCS technologies and their application in enhanced shale gas recovery . - pubmed
( 1972 ) Biochemistry 10 , 2606 - -2617 ) a protein ( radioactive band 4 ) which migrated slightly slower than PAS-3 in SDS gel was labeled heavily by the metal chelate . - pubmed
This protein has an apparent molecular weight of 37,500 in 8.4 % acrylamide - SDS gel . - pubmed
The diazo linkage of the metal chelate to this protein was found to be especially unstable to heat . - pubmed
Brain renin activity was partially purified by ammonium sulfate fractionation and Sephadex gel filtration , resulting in the removal of angiotensinase activity . - pubmed
Both enzyme activities were irreversibly inhibited by the pepsin inhibitor pepstatin , in nanomolar concentrations . - pubmed
Interaction of muscle pyruvate kinase with 1,8 - anilinonaphthalene sulfonate . - pubmed
Adrenalectomy had a small but significant effect on kainate binding in the stratum lucidum and stratum radiatum of CA3 in the first experiment , but no effect in follow - up experiments . - pubmed
However , little is known about how much each type of cell , oxytocin ( OT ) or vasopressin ( VP ) , contributes to this plasticity during dehydration . - pubmed
A method is described for determination of the relative availability of transferrin - bound iron and cell - derived iron to microbial iron - scavenging mechanisms . - pubmed
In one culture the transferrin was labelled with 59Fe and in the other the cells were labelled , and the relative uptake of radioiron by the microorganisms determined . - pubmed
coli was observed , suggesting that differences in the ability of aerobactin and enterochelin to remove iron from intracellular ferritin were not responsible for this preference . - pubmed
Inhibition of epidermal growth factor - induced activation of phospholipase C by psi - tectorigenin . - pubmed
Ovarian response in repetitive cycles induced by menotrophin alone or combined with gonadotrophin releasing hormone analogue . - pubmed
PHA and PPD induced stimulations of DNA synthesis and interleukin-2 ( Il-2 ) production and NK cell activity were measured from peripheral blood mononuclear cells cultured with and without isoprinosine . - pubmed
Rabbit Fc - specific antibodies were conjugated with PNC by a single step glutaraldehyde bridge . - pubmed
The PNC conjugate could be used at a greater dilution than those of the ALP and HRP conjugates and the BTB substrate mixture was stable for at least 3 weeks at 4 degrees C. Penicillin is readily available in developing countries , and at a substantially lower cost than p - nitrophenyl phosphate , the commonly used substrate for ALP in the plate ELISA . - pubmed
Compared with those not so affected , hirsutism was associated with a higher mean serum testosterone concentration , infertility was associated with higher mean gonadotrophin concentrations , obesity was associated with a higher mean serum testosterone concentration , hyperprolactinaemia was associated with a lower mean serum testosterone concentration and smaller ovaries , alopecia was associated with lower mean serum LH and testosterone concentrations , and acanthosis nigricans was associated with obesity and a raised mean serum testosterone concentration . - pubmed
The object of this study was to further explore the suppression of pulsatile LH release by EOPs on day 8 of pregnancy by examining whether inhibition of norepinephrine ( NE ) or epinephrine ( EPIN ) synthesis , or stimulation of gamma - aminobutyric acid ( GABA )- B receptors , modified the ability of naloxone infusion ( 0.5 mg / kg / hr for 3.5 hr ) to stimulate pulsatile LH secretion . - pubmed
1 ) LY 134046 ( PNMT inhibitor , 50 mg / kg IP ) , given at -27 , -20 , and -3 hr relative to the onset of a 3 - hr blood sampling period , produced no change in hypothalamic - preoptic area ( HPOA ) levels of NE , and a 76 % decline in HPOA - EPIN levels , as determined by HPLC . - pubmed
Although basal LH pulse frequency was reduced , this treatment had no effect on the stimulatory action of naloxone on pulsatile LH release . - pubmed
2 ) FLA-63 ( DBH inhibitor , 25 mg / kg IP ) given at -3 hr produced a 72 % decline in HPOA - NE levels , a 44 % decrease in HPOA - EPIN values , and blocked the stimulatory action of naloxone on LH pulse frequency . - pubmed
Since depletion of HPOA - EPIN by LY 134046 did not compromise the LH response to naloxone , this effect of FLA-63 is due to depletion of HPOA - NE levels . - pubmed
3 ) While saline had no effect on the increased pulsatile LH release caused by naloxone , administration of baclofen ( GABA - B receptor agonist , 6 mg / kg IV ) suppressed the pulsatile LH secretory response to naloxone infusion. - pubmed
Synthesis and biological evaluation of phosphonamidate peptide inhibitors of enkephalinase and angiotensin - converting enzyme . - pubmed
3.4.24.11 ) and angiotensin - converting enzyme ( ACE , 3.4.14.1 ) has been explored with a series of enkephalin analogues in which the scissile Gly3 - Phe4 amide bond has been replaced with a phosphonamidate moiety . - pubmed
Within a series of [ ( N - acylamino ) methyl ] phosphonamidates there was a dramatic decrease in inhibitory activity against enkephalinase as the N - acyl moiety was substituted with larger , more hydrophobic acyl groups . - pubmed
Likewise , the inhibitory activity of the [ ( N - acylamino ) methyl ] phosphonamidates against ACE was attenuated by larger phenylalkyl acyl functionalities , although not to the same degree as against enkephalinase . - pubmed
Over a 2 1/2 - year period , treatment with melphalan and prednisolone produced improvement in the neuropathy and resolution of dermato - endocrine features with a corresponding decline in the serum paraprotein concentration . - pubmed
The effect of 3 - aminobenzamide ( 3AB ) , an inhibitor of poly ( ADP - ribose ) synthetase on the frequency of X - ray - induced chromosomal aberrations in these 3 cell lines was also studied . - pubmed
3AB potentiated the frequency of aberrations in G1 and G2 in all the cell types . - pubmed
Vitamin - dependent enzyme patterns ( B1 , B2 , B6 ) and excretion of the tryptophan metabolites kynurenin , xanthurenic acid , trigonellinamide , and N - methylpyridone ] . - pubmed
[ Tinidazol ( CP , 12 - 574 ) in the treatment of vaginal trichomoniasis ] . - pubmed
These data suggest that adult and fetal intestinal alkaline phosphatases represent structurally distinct proteins , either encoded for by different genes or produced by differential processing of a common precursor molecule determined by a single gene . - pubmed
The behavioral disruption associated with this manipulation suggests a role for endogenous LHRH in maintaining sex behavior in a low testosterone environment . - pubmed
2OHE2 - 17 beta and 4 - hydroxyestrone , which are potent estrogens , may act in this instance first as estrogen agonists , advancing the " time window " when their catechol antagonist properties in blocking the LH surge can be functional . - pubmed
The nonuterotropic 2 - hydroxyestradiol-17 alpha and 2 - hydroxyestrone act solely as catechol estrogens and inhibit the preovulatory LH surge if administered at a time when they be present during this physiological window , which is thought to involve events at the estrogen - catecholaminergic interphase . - pubmed
Following administration of bovine growth hormone ( bGH ) and thyroxine ( T4 ) during the first 20 d of postnatal life , this retarded neuronal growth was restored to normal : neuronal perikarya were enlarged , and the dentrites were thicker with denser branchings . - pubmed
Primary autoimmune hypothyroidism was confirmed by the finding of a low serum thyroxine concentration and a high thyrotropin concentration . - pubmed
Sucrose density - gradient ultracentrifugation studies suggest that the mRF- and C1q - reactive substances in both bullous pemphigoid sera and blister fluids are of a size compatible with immune complexes . - pubmed
Functional studies of two new abnormal hemoglobins with their mutation located at intersubunit contacts : Hb Hotel Dieu beta99 ( G1 ) Asp replaced by Gly and Hb Pitie Salpetriere beta34 ( B16 ) Val replaced by Phe . - pubmed
The functional properties of two new abnormal hemoglobins with high oxygen affinity were studied . - pubmed
Hb Hotel Dieu beta99 ( G1 ) Asp replaced by Gly is situated in the alpha1beta2 contact . - pubmed
Both hemoglobins exhibited similar functional properties with a 10 - fold increased oxygen affinity , a decreased cooperativity , a decreased Bohr effect and a normal or slightly decreased 2,3 - diphosphoglycerate effect . - pubmed
Similar effects were observed in ovalbumin - sensitized guinea - pigs upon aerosol of acetaldehyde ( 20 mg / ml ) which has been administered at the flow rate of 0.1 ml / min for 2 min . - pubmed
Theophylline ( CAS 58 - 55 - 9 ) antagonized both the increase of ITP values and the rise of histamine in the blood caused by acetaldehyde given intravenously ( ED50 = 5.8 mg / kg i.v . ) - pubmed
or of the substance P ( SP ) receptor antagonist , [ D - Pro4 , D - Trp7.9 ] SP 4 - 11 ( 10 mg / kg i.v . ) - pubmed
SRF MADS - box position 1 was always a glycine in the variants , but many different sequences at the other nonconserved MADS - box residues were compatible with efficient DNA binding . - pubmed
Additionally , the relative abundance of ligand and receptor in the hIL5.shIL5R alpha complex was determined to be 1:1 by both titration calorimetry and SDS - polyacrylamide gel electrophoresis analysis of dissolved cocrystals of the complex . - pubmed
Measurements of the level of control ( LC ) are available such as glycohemoglobin ( GH ) and fructosamine ( FA ) . - pubmed
In comparison with subjects with good LC , patients with poor LC had higher values of duration of DM ( p & lt ; 0.01 ) , waist / hip ratio ( p & lt ; 0.04 ) , fasting glucose ( p & lt ; 0.001 ) and lower values of fasting insulin ( p & lt ; 0.07 ) , LDL cholesterol ( p & lt ; 0.006 ) and VLDL cholesterol ( p & lt ; 0.09). - pubmed
[ The effect of wheat germ agglutinin on anion transport in the erythrocyte membranes ] . - pubmed
Glycophorin is thought to be associated with Band 3 , the anion transport protein , via the cytoskeleton proteins Band 4.1 , spectrin and ankyrin . - pubmed
The effect of WGA on the anion transport across the erythrocyte membranes was measured by NO2- transport and NH4 Cl isotonic swelling , two different methods developed by our laboratory in recent years . - pubmed
All effects that WGA exerted above were instantly abolished by adding N - acetylglucosamine which has specific binding sites on WGA . - pubmed
In conclusion , the binding of WGA to the sialic acid groups of glycophorin can lead to the conformational changes in saccharides and such changes will be transferred via glycophorin , cytoskeleton to Band 3 , which causes the final change of anion transport . - pubmed
These data indicate that endogenous adenosine inhibits renin release during hemorrhagic hypotension , but does not attenuate noradrenergic neurotransmission even during prolonged hemorrhagic hypotension . - pubmed
Cells incubated with insulin produced 5 - 10 fold more estradiol in response to IGF - II than those incubated without . - pubmed
In contrast , insulin produced only a small increase of estradiol in response to IGF - I. - pubmed
Effects were reversible either by removal of the oligomers or by addition of exogenous IL-8 protein . - pubmed
Intravenous bolus administration of 125 mg / kg of cysteamine induced increases in serum gastrin concentration ( 864 + /- 96 pg / ml ) and gastric acid outputs ( 107 + /- 27 mumol H+/kg.30 min ) not significantly different from the gastrin 17 - l infusion experiments . - pubmed
Immunohistochemistry with antibodies directed against histamine or histidine decarboxylase revealed that hippocampal transplants received the most dense histaminergic innervation . - pubmed
Furthermore , surface iodination , immunoprecipitation and SDS / PAGE analysis were used to identify two annexin I - binding proteins on the surface of monocytes with molecular masses of 15 kDa and 18 kDa respectively . - pubmed
5 - HT3 receptor antagonists such as ondansetron seem to be efficacious both in the control of acute and prolonged emesis following carboplatin chemotherapy , but randomly controlled data comparing ondansetron with other anti - emetic regimens have not yet been published . - pubmed
Plasma thyroid hormone concentrations were significantly ( P & lt ; 0.05 ) lower in WR lambs , but there were no differences between WR and CR groups in thyroid gland weight or hepatic iodothyronine 5 '- deiodinase activity at 8 or 29 days of age . - pubmed
On incorporation of intracellular membrane vesicles containing the delta exon5 CFTR proteins into the lipid bilayer membrane , functional phosphorylation- and ATP - dependent chloride channels were identified . - pubmed
More frequently , the delta exon5 CFTR formed chloride channels with lower conductance states of approximately 2 - 3 and approximately 4 - 6 pS. - pubmed
The data obtained indicate that deleting 30 amino acids from the first intracellular loop of CFTR affects both processing and function of the CFTR chloride channel . - pubmed
We describe a reverse transcription polymerase chain reaction ( RT - PCR ) technique using fluorescent dUTP to examine changes in mRNA level in samples . - pubmed
Testosterone treatment of females caused irregular inhibitory effects on the FHG22 mRNA levels , whereas male castration originated transitory increases during less than 2 weeks . - pubmed
Treatment of 15 day - castrated males for 1 or 2 days with beta - estradiol-3 - benzoate caused a marked stimulation in the FHG22 mRNA levels . - pubmed
In the absence of hormones , the FHG22 mRNA levels decreased along the time and neither progesterone , testosterone , or 5 alpha - dihydrotestosterone affected the expression . - pubmed
However , estradiol stimulated the FHG22 mRNA expression in a time and dose - dependent manner : increasing effects were detected between 8 - 96 h of treatment and the EC50 was about 10(-9 ) M. The estradiol effect was reverted by the antiestrogen ICI 164,384 or by cycloheximide . - pubmed
We conclude that estradiol stimulates FHG22 mRNA expression in Harderian gland , although other agents may also control the expression in vivo . - pubmed
The induction of prostaglandin G / H synthase ( PGHS ; prostaglandin endoperoxide synthase , cyclooxygenase ) by proinflammatory cytokines accounts , at least in part , for the altered eicosanoid biosynthesis in inflammatory diseases . - pubmed
The enhanced production of PGE2 reflected the upregulation of PGHS-2 as indicated by enhanced expression of PGHS-2 RNA and increased recovery of PGHS-2 protein in NHBECs . - pubmed
When the binding between the epidermal growth factor ( EGF ) receptor and its ligands was disrupted by a neutralizing antibody ( LA-1 ) , IFN - gamma failed to upregulate the release of PGE2 and the expression of PGHS-2 RNA in NHBECs . - pubmed
Furthermore , IFN - gamma induced the expression of RNAs for a number of ligands at the EGF receptor TGF - alpha ; heparin - binding EGF - like growth factor ( HB - EGF ) ; and amphiregulin in NHBECs , and when administered exogenously , these ligands increased PGE2 release from NHBECs . - pubmed
These data are consistent with the presence of an autocrine growth factor / EGF receptor loop regulating PGHS-2 expression and PGE2 synthesis in bronchial epithelial cells . - pubmed
Induction of calreticulin expression in response to amino acid deprivation in Chinese hamster ovary cells . - pubmed
We characterize here the induction of calreticulin in response to complete amino acid deprivation in Chinese hamster ovary cells . - pubmed
Amino acid deprivation caused a 4 - fold increase in calreticulin protein levels over a period of 4 - 10 h. In addition to an overall increase in protein levels , the glycosylation of calreticulin was increased . - pubmed
This glycosylation event was blocked by tunicamycin and was not required for the increase in calreticulin protein levels . - pubmed
These results are similar to the large increases in asparagine synthetase mRNA observed in response to amino acid deprivation , but the amino acid - deprivation - response element identified to be involved in asparagine synthetase induction is absent from the calreticulin promoter . - pubmed
The release of AMB from the fatty base of Witepsol or a medium chain triglyceride ( MCT ) was markedly faster than that from the hydrophilic base of macrogol . - pubmed
The addition of POE ( 2 ) LE into the MCT base resulted in a marked increase in bioavailability , showing the highest bioavailability of 4.9 % . - pubmed
Solid state NMR studies of hydrogen bonding in a citrate synthase inhibitor complex . - pubmed
The ionization state and hydrogen bonding environment of the transition state analogue ( TSA ) inhibitor , carboxymethyldethia coenzyme A ( CMX ) , bound to citrate synthase have been investigated using solid state NMR . - pubmed
This enzyme - inhibitor complex has been studied in connection with the postulated contribution of short hydrogen bonds to binding energies and enzyme catalysis : the X - ray crystal structure of this complex revealed an unusually short hydrogen bond between the carboxylate group of the inhibitor and an aspartic acid side chain [ Usher et al . - pubmed
To further investigate the nature of this short hydrogen bond , low spinning speed 13C NMR spectra of the CMX - citrate synthase complex were obtained under a variety of sample conditions . - pubmed
Thus the tensor values for the enzyme - inhibitor complex could be consistent with either a very long syn hydrogen bond or an anti hydrogen bond ; the latter would agree very well with previous crystallographic results . - pubmed
This proton resonance is assigned to Hdelta2 of Asp 375 , on the basis of comparison with crystal structures and the fact that this cross - peak was absent in the heteronuclear correlation spectrum of the inhibitor - D375 G mutant enzyme complex . - pubmed
The cellular redox status is controlled primarily by glutathione , a major cellular antioxidant , whose synthesis is regulated by the rate - limiting enzyme gamma - glutamylcysteine synthetase ( gamma - GCS ) . - pubmed
gamma - GCS overexpression also completely suppressed NF - kappa B activation induced by phorbol ester and okadaic acid , whereas that induced by H2O2 , ceramide , and lipopolysaccharide was minimally affected . - pubmed
Overall , our results indicate that most of the pleiotropic actions of TNF are regulated by the glutathione - controlled redox status of the cell . - pubmed
The enzyme released acetate from several acetylated substrates , including acetylated xylan . - pubmed
Using p - nitrophenyl acetate as a substrate , the enzyme had a K(m ) of 0.9 mM and a V(max ) of 785 micromol min(-1 ) mg(-1 ) . - pubmed
ADP - ribosylation factor ( ARF ) 6 regulates plasma membrane trafficking and cortical actin formation by cycling between inactive GDP and active GTP - bound conformations . - pubmed
We also describe experiments that propose a central role in this pathway for the PI 3 - kinase - dependent plasma membrane recruitment of the cytohesin-1 family of PtdIns ( 3,4,5 ) P ( 3 )-binding ARF - exchange factors . - pubmed
Agonists ( isoproterenol or nitroprusside ) were used to stimulate adenylyl cyclase and guanylyl cyclase , respectively , in the absence and presence of nonselective IBMX or PDE5 specific inhibitors E4021 ( 1 ) . - pubmed
The PDE activities reside predominantly in the supernatant , but the PDE activity for degrading cGMP is more pronounced . - pubmed
Moreover , results with E4021 suggest that PDE5 activity could play a critical role in modulating cGMP - related activity in the trabecular meshwork . - pubmed
An accumulation of extracellular lactate was observed after inhibition of the lactate transporters suggesting that transport of lactate into neurones is necessary for the neuroprotective effect of 17beta - estradiol . - pubmed
These results suggest a new neuroprotective mechanism of 17beta - estradiol by activating glutamate - stimulated lactate production , which is estrogen receptor - dependent . - pubmed
Our results show that a single intraventricular administration of HIVgp120 selectively increases REMS and that such an increase can be prevented by U0126 , an inhibitor of ERK activating enzyme , MEK . - pubmed
In contrast , SB202190 , a MAPK - p38 inhibitor , had no effect on HIVgp120 - induced increase in REMS . - pubmed
We have previously reported a single nucleotide polymorphism ( SNP ) at nucleotide ( nt ) position -88 ( G or T ) within an interferon - stimulated response element - like sequence in the promoter region of the MxA gene , which correlated with responsiveness of hepatitis C patients to interferon . - pubmed
The case of the platelet glycoprotein IIb / IIIa inhibitor , abciximab , is used to illustrate many of the issues surrounding the collection , interpretation , and ( mis ) application of cost - effectiveness data . - pubmed
The neuronal serine protease inhibitor neuroserpin is widely expressed in the developing and adult brain . - pubmed
Measurement of multiple psi torsion angles in uniformly 13C,15N - labeled alpha - spectrin SH3 domain using 3D 15N-13C-13C-15N MAS dipolar - chemical shift correlation spectroscopy . - pubmed
We demonstrate the simultaneous measurement of several backbone torsion angles psi in the uniformly ( 13 ) C ,( 15 ) N - labeled alpha - Spectrin SH3 domain using two different 3D 15N-13C-13C-15N dipolar - chemical shift magic - angle spinning ( MAS ) NMR experiments . - pubmed
BACKGROUND : Interleukin ( IL )- 18 has been shown to activate basophils to produce histamine and IL-4 and to induce naive T cells to differentiate into T - helper ( Th ) 2 cells . - pubmed
The performance of the nonadentate chelate was compared with a heptadentate chelate in a dry reagent immunoassay for human chorionic gonadotropin ( hCG ) . - pubmed
Contrary to the heptadentate label , the effect of aqueous quenching on the nonadentate label was found to be insignificant , with calculated analytical detection limits ( background + 3 SD ) of 0.9 and 0.7 IU / L hCG for wet and dry measurements , respectively , and a linear range up to 5000 IU / L. The CVs for the new label were & lt;8 % at the cutoff of 25 IU / L and above in both whole blood and plasma . - pubmed
Copper sensitized astrocytes to the action of menadione , an intracellular generator of superoxide anion radical , exogenous hydrogen peroxide ( H2O2 ) and rotenone , an inhibitor of mitochondrial electron transport chain complex I. - pubmed
Four electrode materials : Pt , Au , Pd and glassy carbon ( GC ) , were studied to investigate their suitability as substrates in the development of two different classes of glutamate biosensor . - pubmed
Glutamate oxidase cross - linked onto poly ( o - phenylenediamine ) was chosen as the type 1 biosensor ( PPD / GluOx ) , incorporating PPD as the permselective element to detect H ( 2 ) O ( 2 ) directly on the electrode surface at relatively high applied potentials . - pubmed
GluOx and horseradish peroxidase / redox polymer modified electrodes ( Os ( 2 +) PVP / HRP / GluOx ) that relied on enzyme - catalysed H ( 2 ) O ( 2 ) detection at lower applied potentials were used as type 2 biosensors . - pubmed
The voltammetric and amperometric responses to the enzyme signal transduction molecule , H ( 2 ) O ( 2 ) , and the archetypal interference species in biological applications , ascorbic acid , were determined on the bare and PPD / GluOx - modified surfaces . - pubmed
Cadherins are Ca ++-dependent cell - cell adhesion molecules which play an important role in the cellular connection between normal cells . - pubmed
cynocephalus ) were autotransfused with RBCs that had been harvested using CPD and that had been resuspended and stored in AS-1 solution at 4 degrees C for as long as 49 days . - pubmed
Phase II / III trials should be done to compare docetaxel with liposomal anthracyclines as a first - line treatment . - pubmed
Niemann - Pick disease type C ( NPC ) is a genetic disorder in which patient cells exhibit lysosomal accumulation of cholesterol and sphingolipids ( SLs ) caused by defects in either NPC1 or NPC2 proteins . - pubmed
The clearance of lysosomal free cholesterol was also associated with a decrease in LDL - receptor levels . - pubmed
Reduction of naphthoquinones by DT - diaphorase is often described as a detoxification reaction . - pubmed
In the present study , the effect of several substances that are known to increase tissue activities of DT - diaphorase on the toxicity of 2 - amino-1,4 - naphthoquinone has been investigated . - pubmed
Again like 2 - hydroxy-1,4 - naphthoquinone , the severity of the haemolysis induced by the 2 - amino derivative was increased in animals pre - treated with inducers of DT - diaphorase , but the degree of nephrotoxicity was decreased . - pubmed
With these substances , therefore , DT - diaphorase both activates and detoxifies the quinone , depending on the target organ . - pubmed
It is not possible to generalize with regard to the effects of modulation of tissue levels of DT - diaphorase on naphthoquinone toxicity in vivo , since this may change not only the severity of the toxic effects , but also the target organ specificity . - pubmed
These clusters include one cluster ( Mal I , PF1737 to PF1751 ) involved in maltose metabolism and another cluster ( PF0691 to PF0695 ) whose products are thought to remove toxic reactive nitrogen species . - pubmed
The results obtained demonstrate that MC - NO : ( a ) absorbs in the UVB region even after UVA irradiation ; ( b ) acts as free radical scavenger as demonstrated by EPR experiments ; ( c ) strongly reduces both UVA- , sunlight- and BMDBM - induced lipid peroxidation in liposomes , measured as reduced TBARS levels ; and ( d ) has comparable antioxidant activity to that of commonly used vitamin E and BHT in skin care formulations . - pubmed
Ca2 + sparks are the elementary units of intracellular Ca2 + signaling in striated muscle cells revealed as localized Ca2 + release events from sarcoplasmic reticulum ( SR ) by confocal microscopy . - pubmed
Adsorption , ionization , and migration of hydrogen chloride on ice films at temperatures between 100 and 140 K. Adsorption of hydrogen chloride ( HCl ) on water ice films is studied in the temperature range of 100 - 140 K by using Cs+ reactive ion scattering ( Cs+ RIS ) , low energy sputtering ( LES ) , and temperature - programmed - desorption mass spectrometry ( TPDMS ) . - pubmed
Falsely low LDL cholesterol results and cholestasis . - pubmed
We have identified two new histone deacetylase ( HDAC ) inhibitors ( 9 and 24 ) capable of inducing the expression of gamma - globin and/or beta - globin promoter - driven reporter genes in a synthetic model of Hb switch . - pubmed
nBAF complexes bind tightly to the Ca ( 2 +)-responsive dendritic regulator CREST and directly regulate genes essential for dendritic outgrowth . - pubmed
Recent work strongly suggests that NADPH oxidase is a major source of superoxide in cardiovascular cells , and oxidative stress can be involved in the process of endothelial dysfunction . - pubmed
From the viewpoint of these molecular mechanisms , HMG - CoA reductase inhibitors ( statins ) might inhibit the high glucose - induced NADPH oxidase activation through inhibition of Rac activity and finally prevent the increase in ROS production in diabetes . - pubmed
Beta - blockers decrease liver perfusion and inhibit the activity of hepatic microsomal lidocaine metabolizing enzymes of the P450 _ 2D subfamily . - pubmed
After making a mark on the stromal side of a microkeratome - prepared DSAEK corneal button to confirm appropriate orientation of the donor tissue after insertion into the anterior chamber , the corneal tissue was returned to Optisol GS solution for 1 hour . - pubmed
The glutathione peroxidase ( GPx ) activities of some diaryl diselenides incorporating tertiary amino groups were studied with H ( 2 ) O ( 2 ) , Cum - OOH , and tBuOOH as substrates and with PhSH as thiol co - substrate . - pubmed
Simple replacement of a hydrogen atom with a methoxy group dramatically enhances the GPx activity . - pubmed
The chemical identities of these components were confirmed by ( 1 ) H NMR and EI - MS . - pubmed
Aromatase inactivation by 2 - substituted derivatives of the suicide substrate androsta-1,4 - diene-3,17 - dione . - pubmed
To gain the structure - activity relationship of Delta ( 1 )- androstenediones ( Delta ( 1 )- ADs ) as mechanism - based inactivator of aromatase , series of 2 - alkyl - and 2 - alkoxy - substituted Delta ( 1 )- ADs ( 6 and 9 ) as well as 2 - bromo - Delta ( 1 )- AD ( 14 ) were synthesized and tested . - pubmed
All of the alkyl steroids 6 along with the alkoxy steroid 9 , except for the ethyl and n - propyl compounds 6b and 6c , caused a time - dependent inactivation of aromatase . - pubmed
The results indicate that the 2 - hexyl compound 6f act as the most powerful mechanism - based inactivator of aromatase among Delta ( 1 )- AD analogs and may be submitted to the preclinical study in estrogen - dependent breast cancer . - pubmed
Due to the presence on histology of lymphoid follicles and the persistent high serum immunoglobulin E levels , we elected to attempt treatment with rituximab . - pubmed
The efficacy of flaxseed oil supplementation was investigated in terms of survival of animals and production of various inflammatory molecules ( malondialdehyde , myeloperoxidase , nitric oxide ) in the lung homogenate of animals . - pubmed
This was accompanied by decreased inflammatory markers ( malondialdehyde , myeloperoxidase , nitric oxide ) as the disease progressed . - pubmed
Yeast Elongation protein 3 ( yElp3 ) , the catalytic subunit of the multi - subunit histone acetyltransferase elongator complex , is involved in histone acetylation and transcription , exocytosis and tRNA modification . - pubmed
For immunoassay , specificity can be an issue especially in relation to the proportion of 25OHD2 that is quantified whereas HPLC and LC - MS / MS methods are able to measure the two major vitamin D metabolites 25OHD2 and 25OHD3 independently . - pubmed
The tyrosine kinase receptor RET51 is expressed in distinct families of neurons where it promotes different functions . - pubmed
The substitution of the tyrosine 905 of RET51 , a key residue phosphorylated by both GDNF and NGF , disrupts the RET51 / FKBP52 complex . - pubmed
Response of perianal fistulas to antibiotics ( metronidazole alone or combined with ciprofloxacin ) was significantly higher in patients without CARD15 mutations ( 7.7 % versus 40.5 % , P = 0.041 ) . - pubmed
However , significant decreases of plasma and ovarian inhibin alpha were seen after reinstatement of estrous cyclicity with progesterone implant treatment . - pubmed
Investigational approaches discussed include aminothienopyridazine inhibitors of tau aggregation , the alternative splicing of tau pre - mRNA , protein phosphatase 2A as a potential therapeutic target , and immunotherapy and macroautophagy approaches for clearing aberrant tau protein from the brain . - pubmed
The combined structural and mutagenesis results suggest that during decarboxylation of ferulic acid by FADase , Trp25 and Tyr27 are required for the entering and proper orientation of the substrate while Glu134 and Asn23 participate in proton transfer . - pubmed
The aim of the current study was to explore in vitro the effects of STP on neuronal activity of GP neurons during local repetitive stimulation . - pubmed
Nephropathy was defined as microalbuminuria ( urinary albumin - creatinine ratio [ ACR ] : 30 to 299 mg / g ) , macroalbuminuria ( urinary ACR : & gt;= 300 mg / g ) , or an estimated glomerular filtration rate of & lt;60 mL / min/1.73 m(2 ) . - pubmed
Ouabain - sensitive and ouabain - resistant Rb+ ( K+ ) influxes were measured during regulatory cell volume increase ( RVI ) and apoptotic volume decrease ( AVD ) . - pubmed
Here , we demonstrate that while replication factor C subunit 1 ( RFC1)-RFC is required for Cdt1 degradation after UV irradiation during the nucleotide excision repair process , another RFC complex , Ctf18 - RFC , which is known to be involved in the establishment of cohesion , has a key role in Cdt1 degradation in S phase . - pubmed
Mitochondrial neurogastrointestinal encephalopathy ( MNGIE ) is caused by deficiency in thymidine phosphorylase ( TP ) , that regulates thymidine ( dThd ) and deoxyuridine ( dUrd ) . - pubmed
, IV - L - glutamine ( 250 mg / kg , p.o . ) - pubmed
Glycosylated haemoglobin , lipid profile , plasma and colonic active ( GLP-1 ) ( 7 - 36 ) amide , mRNA expression of proglucagon GLP-1 , plasma and pancreatic insulin , histology of pancreata and biomarkers of oxidative stress ( superoxidase dismutase , reduced glutathione , malondialdehyde , glutathione peroxidase , glutathione S transferase ) were measured after 8 week . - pubmed
, II - sitagliptin ( 5 mg / kg , p.o . ) - pubmed
, IV - L - glutamine ( 500 mg / kg , p.o . ) - pubmed
Plasma glucose , active GLP-1 ( 7 - 36 ) amide concentration and insulin levels were measured after glucose loading . - pubmed
The docking data indicated that l - glutamine bind to the GLP-1 receptor . - pubmed
In this study we labeled the anti - human epidermal growth factor receptor type 2 ( HER2 ) Nanobody with ( 68 ) Ga via a 1,4,7 - triazacyclononane-1,4,7 - triacetic acid ( NOTA ) derivative and assessed its use for HER2 iPET imaging . - pubmed
Biodistribution studies showed fast and specific uptake ( percentage injected activity [ % IA ] ) in HER2 - positive tumors ( 3.13 + - 0.06 and 4.34 + - 0.90 % IA / g for ( 68 ) Ga - NOTA-2Rs15dHis6 and ( 68 ) Ga - NOTA-2Rs15d , respectively , at 1 h after injection ) and high tumor - to - blood and tumor - to - muscle ratios at 1 h after injection , resulting in high - contrast PET / CT images with high specific tumor uptake . - pubmed
CONCLUSION : A new anti - HER2 PET tracer , ( 68 ) Ga - NOTA-2Rs15d , was synthesized via a rapid procedure under mild conditions . - pubmed
Up - regulation of urinary markers predict outcome in IgA nephropathy but their predictive value is influenced by treatment with steroids and azathioprine . - pubmed
OBJECTIVE : Steroids and immunosuppressants can delay progression of renal function in IgAN , but their possible effect in local cytokines has not been studied . - pubmed
Serum creatinine at time of diagnosis had significant correlation with proteinuria ( p = 0.02 ) , urinary levels of IL-1beta ( p = 0.03 ) , IL-2 ( p = 0.01 ) and MCP-1 ( p = 0.03 ) . - pubmed
Steroids + aza may exert their beneficial effect through suppression of the production or activation of most cytokines . - pubmed
Under both unstimulated and RANKL - stimulated conditions , Nrf2 loss led to an increase in the intracellular ROS level and the oxidized - to - reduced glutathione ratio and a defect in the production of numerous antioxidant enzymes and glutathione . - pubmed
Moreover , pretreatment with N - acetylcysteine or diphenyleneiodonium significantly reduced the OC differentiation and decreased the intracellular ROS level in both Nrf2 - deficient and wild - type cells . - pubmed
Pretreatment with sulforaphane and curcumin also inhibited the OC differentiation by activating Nrf2 in part . - pubmed
RESULTS : In GI and GII , addition of plerixafor led to a significant ( p & lt ; 0.01 ) increase of leukocytes and CD34 + cells in peripheral blood ( PB ) . - pubmed
In GII , the median number of CD34 + cells in PB before and after addition of plerixafor was significantly ( p = 0.019 ) higher compared to GI ( 9 vs. 5 and 50 vs. 24 cells / mul , respectively ) . - pubmed
CONCLUSIONS : Plerixafor and G - CSF mobilization or the addition of plerixafor during non - optimal chemotherapy and G - CSF mobilization together with LVL enabled , independent of leukocyte count and even without detectable CD34 + cells before addition of plerixafor , sufficient harvest of HPC numbers for transplantation . - pubmed
Mechanism of action of colchicine : IV . - pubmed
Embryogenesis was induced with three genotypes using a modified half - strength Murashige and Skoog ( MS ) medium supplemented with 1.0 to 4.0 mg l(-1 ) 2,4 - dichlorophenoxyacetic acid ( 2,4 - D ) and 0.33 to 1.0 mg l(-1 ) kinetin . - pubmed
Within two to three months after culture initiation , embryos were transferred to modified MS medium containing 0.2 mg l(-1 ) 6 - benzyladenine ( BA ) and 2 % sucrose and placed in the light for conversion into plantlets . - pubmed
It is concluded that the NAA - binding - sites are probably located at the external face of the plasma membrane ; they are destroyed during protoplast isolation by proteolytic enzymes in the cellulase and macerozyme preparations . - pubmed
The mitochondria promote branching through ATP generation and the determination of localized hot spots of active axonal mRNA translation , which contribute to actin - dependent aspects of branching . - pubmed
Group A - Bioactive glass ( SHY - NM ) , Group B - Fluoride tooth paste ( Amflor ) , Group C - CPP - ACP ( Tooth mousse ) , Group D - CPP - ACPF ( Tooth mousse plus ) , Group E - control . - pubmed
Results showed that the retinoic acid receptor alpha ( RARalpha ) , a target protein in acute promyelocytic leukemia ( APL ) , was in the top rank , with a pharmacophore model matching well the molecular features of Tanshinone IIA . - pubmed
These results indicated that RARalpha may be a potential target of Tanshinone IIA for APL . - pubmed
From our nucleosome model , it is evident that substitution of specific amino acid residues in TH2B results in both differential histone - DNA and histone - histone contacts . - pubmed
Recognition of foreign antigens by T lymphocytes is restricted by molecules of the major histocompatibility complex ( MHC ) . - pubmed
The technique of Texas Red immunostaining for cellular gammaH2AX identifies by fluorescence microscopy DNA undergoing active remodeling or repair . - pubmed
Energy metabolism genes encoded proteins primarily involved in H2 uptake and electron cycling , including a rhodanese - containing complex that is phylogenetically conserved among sulfate - reducing Deltaproteobacteria . - pubmed
Exposure to 1,4 - BQ showed no significant effect on CD3e(+ ) cells but reduced the total counts of Sca-1(+ ) , CD11b(+ ) , Gr-1(+ ) , and CD45(+ ) cells at 7 and 12 muM ( p & lt ; 0.05 ) . - pubmed
Skeletal muscles continuously produce reactive oxygen species ( ROS ) and reactive nitrogen species ( RNS ) at rest and more so during contraction . - pubmed
Morphine is a substrate of P - glycoprotein and the P - glycoprotein - mediated efflux transport at the BBB maintains a lower unbound concentration of morphine in the brain compared with plasma . - pubmed
Unlike EM , AFM imaging can be performed under physiological conditions , enabling the real - time visualization of PFT assembly and the transition from the prepore state , in which the toxin does not span the membrane , to the pore state . - pubmed
In addition to characterizing PFT oligomers , AFM has also been used to examine toxin - induced alterations in membrane organization . - pubmed
In this review , we summarize the contributions of AFM to the understanding of both PFT assembly and PFT - induced membrane reorganization . - pubmed
Serum lactate and base deficit are very sensitive markers for detecting and monitoring the extent of bleeding and shock , in conjunction with repeated combined measurements of prothrombin time , activated partial thromboplastin time , fibrinogen and platelets . - pubmed
The methylation of SINEs is increased compared with their flanking regions , and the strongest effect is visible for cytosines in the CHH context , indicating an involvement of asymmetric methylation in the silencing of SINEs . - pubmed
The growth inhibitory effect of these lectins on pancreatic cancer cells were shown to be a consequence of lectin cell surface binding and triggering G0 / G1 arrest , mitochondrial membrane depolarization , sustained increase of the intracellular calcium release and the apoptotic signal is amplified by activation of caspases executing cell death . - pubmed
Both OS ( hazard ratio ( HR)=1.14 , 95 % CI 0.80 - 1.63 , P=0.448 ) and PFS ( HR=1.20 , 95 % CI 0.95 - 1.52 , P=0.119 ) were similar in the erlotinib compared to the chemotherapy group using IPW - adjusted Cox regression analysis treating the use of erlotinib as a time - dependent covariate starting from second - line treatment and stratified for ECOG performance status and treatment line . - pubmed
F1F0 ATP synthases are bidirectional molecular motors that translocate protons across the cell membrane by either synthesizing or hydrolyzing ATP . - pubmed
Unlike mesophilic ATP synthases , alkaliphilic enzymes have tightly regulated ATP hydrolysis activity , which can be relieved in the presence of lauryldimethylamine oxide . - pubmed
In addition , epigenetic analysis revealed a differential methylation pattern in TPO , and triiodothyronine tissue levels were significantly decreased ( P<0.01 ) . - pubmed
Adjustment for E2F1 expression attenuated the association of ASK1 with fasting glucose , insulin resistance , circulating IL-6 , and lipids ( triglycerides , HDL - cholesterol ) , even after adjusting for BMI . - pubmed
An innovative MWCNTs / DOX / TC nanosystem for chemo - photothermal combination therapy of cancer . - pubmed
We previously developed an innovative TAT - chitosan functionalized MWCNTs ( MWCNTs / TC ) based drug delivery system for doxorubicin ( DOX ) and preliminarily investigated its release profile and antitumor effect . - pubmed
In the present study , the application potential of MWCNTs / DOX / TC in chemo - photothermal combination therapy was further explored . - pubmed
The in vivo anti - tumor effect of MWCNTs / DOX / TC was further evaluated by noninvasive bioluminescence imaging . - pubmed
Using commercially available nanoparticles , continuous wave Surface - Enhanced Stimulated Raman spectroscopy ( CW SE - SRS ) is demonstrated for the first time using two Ti : Sapphire lasers producing a pump beam ( 785 nm , 100 mW ) and appropriately varying probe / Stokes beams ( 860 - 870 nm , 120 mW ) . - pubmed
Removal of lead ( II ) from aqeouos waste using ( CD - PCL - TiO2 ) bio - nanocomposites . - pubmed
In this study , titanium dioxide was synthesized via sol - gel technique then incorporated in a polymer blend ( CD - PCL ) via solution blending method . - pubmed
HS is treated with the IL-1 Receptor antagonist ( anakinra ) but also specifically with IL-18BP . - pubmed
In addition , results showed that the architecture and distribution of grafted polyNaSS on Ti surfaces alter the intensity of the bacteria response mediated by fibronectin . - pubmed
Interestingly , H12 contains a distinct cluster of cis - elements like dehydration - responsive element ( DRE - core ; GCCGAC ) , an ABA - responsive element ( ABRE ; ACGTGTC ) and as-1 element ( TGACG ) which are known to be induced by cold , drought and pathogen / SA respectively . - pubmed
All WU conditions reduced the energy cost ( EC ) of running , respiratory exchange ratio , and step frequency ( SF ) after the experimental and control phases of WU , while blood lactate ( BLC ) increased in uphill and level WUs and decreased in control WUs . - pubmed
This protein exists as a dimer of two identical subunits and contains two intramolecular disulfide bonds that are responsible for its resistance to trypsin digestion and allergenicity . - pubmed
Based on SDS - PAGE , HPLC analysis , and competitive ELISA , the reduction of disulfide bonds of BLG with OsNTRB / OsTrx23 , OsNTRB / OsTrx1 , GSH / OsTrx1 , or GSH / OsTrx20 increased its trypsin digestibility and reduced its immunoreactivity . - pubmed
In the hypostome , HmTSP- and Wnt3 - expressing cells were identical or in close vicinity to each other , and regions of ectopic tentacle formation induced by pharmacological beta - Catenin activation ( Alsterpaullone ) corresponded to foci of HmTSP expression . - pubmed
TlpA has been implicated in sensing arginine , bicarbonate , and acid , but no experimentally determined protein structures of TlpA were available to better understand ligand binding and signal transduction . - pubmed
Comparisons with TlpC show that despite high similarity across the general structure , TlpA lacks the residues required to bind lactate , and instead contains a pocket almost entirely hydrophobic in nature . - pubmed
Cytochrome P450 ( Cyp ) 17A1 has both 17alpha - hydroxylase and 17,20 - lyase activities , which are involved in the steroidogenic pathway that produces androgens and estrogens . - pubmed
These results confirm that adequate estrogen is essential for maintaining ovarian differentiation , and they provide new insight into the role of FSHbeta in male testicular development and spermatogenesis . - pubmed
The toxins comprise 73 amino acid residues and 5 disulfide bridges , being homologous to alpha - bungarotoxin ( alpha - BgTx ) , a classical blocker of muscle - type and neuronal alpha7 , alpha8 , and alpha9alpha10 nAChRs . - pubmed
On the other hand , the hydroxyls groups available on MSNs surface allows the conjugation of specific molecules which can binds to the overexpressed Enhanced Growth Factor Receptor ( EGFR ) in many tumors , representing a potential strategy for the cancer treatment . - pubmed
Monoamine receptor interaction profiles of 4 - aryl - substituted 2,5 - dimethoxyphenethylamines ( 2C - BI derivatives ) . - pubmed
Many ring - substituted phenethylamines exert psychedelic effects that are thought to be primarily mediated by interactions with serotonergic 5 - hydroxytryptamine 2 ( 5 - HT2A ) receptors . - pubmed
In contrast , 2C derivatives with bulky lipophilic substituents at the 4 - position of the phenyl ring tend to display antagonist behavior at serotonin 5 - HT2 receptor sites . - pubmed
To gain a better understanding of agonist and antagonist behavior of substituted phenethylamines , binding affinities and functional activation and inhibition of a series of 4 '- aryl substituted 2,5 - dimethoxyphenethylamine ( 2C - BI derivatives ) at various monoamine receptors were determined . - pubmed
Additionally , 2C - BI-8 and 2C - BI-12 activated serotonin 5 - HT2A and 5 - HT2B receptors at submicromolar concentrations . - pubmed
2C - BI-1 and 2C - BI-7 were the only 2C - BI derivatives to activate human trace amine - associated receptor 1 . - pubmed
2C - BI-3 and 2C - BI-4 interacted with monoamine transporters but with low overall potency . - pubmed
The relatively high affinities of various 2C - BI derivatives at the serotonin 5 - HT2A receptor indicate a high steric tolerance of the binding pocket . - pubmed
Potent partial activation of the serotonin 5 - HT2A receptor by 2C - BI-8 and 2C - BI-12 suggests that these substances may potentially exert psychedelic effects similar to other compounds of the 2C family . - pubmed
The 2 - year RFS and overall survival rates for DCF versus ACF were 64.1 % versus 42.9 % ( hazard ratio 0.53 , 95 % confidence interval 0.33 - 0.83 , P = 0.0057 ) and 78.6 % versus 65.4 % ( P = 0.08 ) , respectively . - pubmed
Degradation of DNA by DNase 1 was monitored at a steady state by making changes in the fluorescence polarisation of EtBr in droplets with a volume of 330 picolitre at a 40 hertz frequency under visible light . - pubmed
Using this technique , we successfully determined the half - maximal inhibitory concentration ( IC50 ) of ethylenediaminetetraacetic acid ( EDTA ) for the inhibition of DNase 1 activity to be 1.56 + - 0.91 mM. - pubmed
We halted autophagy by introducing Atg 5 specific siRNA or the autophagy inhibitor Bafilomycin A1 ( Baf - A1 ) . - pubmed
Treatment with IKK16 ( 1 mg / kg body weight ) was administered , by tail vein , 15 min post - LPS . - pubmed
At 24 h post - IKK16 treatment , serum enzyme levels indicative of liver , kidney , pancreas and muscle function were evaluated by biochemical analysis , and RT - PCR technique was used to analyze gene expression of pro - inflammatory cytokines . - pubmed
Moreover , as expected , LPS promoted a significantly overexpression of TNF - alpha , IL-6 and IL-1beta in the heart , kidney , and liver ; which was diminished by IKK16 treatment . - pubmed
However , further studies on cardiac biomarkers and other diagnostic tools in CO poisoning are needed given the low sensitivity of troponin I. - pubmed
METHODS : A well designed single - center cross - sectional study was performed and the GFR was determined by 3 methods separately in the same day : technetium-99m - diethylene triamine pentaacetic acid ( 99mTc - DTPA ) dual plasma sample clearance method ( mGFR ) ; FAS equation method ; Asian modified CKD - EPI equation method . - pubmed
Both the FAS equation and Asian modified CKD - EPI can be a satisfactory method and may be the most suitable creatinine - based equation . - pubmed
Pygenic Acid A ( PA ) Sensitizes Metastatic Breast Cancer Cells to Anoikis and Inhibits Metastasis In Vivo . - pubmed
In this process , dyslipidemia indexes ( LDL , apolipoproteins A ) correlate with the calcium volume whereas blood glucose , as measured in the oral glucose tolerance test , correlates with the lipid component of coronary plaque . - pubmed
had a high proportion of potential genes responsible for methanogenesis and nitrogen fixation , whereas the CAB of Temora spp . - pubmed
At the 6th week of intervention , glucose contents of intraperitoneal glucose tolerance test ( IPGTT ) and intraperitoneal insulin tolerance test ( IPITT ) were measured . - pubmed
Serum total cholesterol ( TC ) and total triglyceride ( TG ) were assayed by using an automatic biochemical analyzer . - pubmed
hydrolases with particular reference to pyrophosphate and orthophosphate monoester phosphohydrolases . - pubmed
The predominant pathway for ethanol oxidation at low ethanol concentrations involves alcohol dehydrogenase . - pubmed
Oxidation of ethanol under these conditions is associated with a change in the steady - stage concentration of catalase - H2O2 . - pubmed
Based on recent evidence , we conclude that it is unnecessary to postulate that ethanol is oxidized directly via cytochrome P-450 . - pubmed
Acetaldehyde production from ethanol via the microsomal subfraction can be accounted for by the combined activities of catalase - H2O2 and alcohol dehydrogenase . - pubmed
The metabolism of ehtanol via alcohol dehydrogenase produces a marked reduction in the hepatocellular NAD - NADH sytems . - pubmed
Attempts in inhibit alcohol dehydrogenase with alkylpyrazoles and activate catalase with substrates for peroxisomal H2O2 - generating flavoproteins , while successful , may have limited applicability because of the native toxicity of the substrates themselves ... - pubmed
Coexistence of contractile and relaxant 5 - hydroxytryptamine receptors coupled to distinct signaling pathways in intestinal muscle cells : convergence of the pathways on Ca2 + mobilization . - pubmed
In naive cells from both regions , 5 - HT caused contraction and stimulated increases in cytosolic free calcium concentration ( [ Ca2+]i ) ( 3 - fold ; p & lt ; 0.01 ) and cAMP levels ( 40 - 60 % ; p & lt ; 0.01 ) that were inhibited , respectively , by the 5 - HT2 antagonist ketanserin and the 5 - HT1p antagonist N - acetyl-5 - hydroxytryptophyl 5 - hydroxytryptophan amide ( 5 - HTP - DP ) . - pubmed
In cells maximally contracted with a non-5 - HT agonist ( cholecystokinin octapeptide ) , 5 - HT caused relaxation when the contractile effect mediated by 5 - HT2 receptors was blocked with ketanserin ; relaxation and the concomitant increase in cAMP were inhibited by 5 - HTP - DP . - pubmed
In cells with only 5 - HT2 receptors , 5 - HT caused contraction and an increase in [ Ca2+]i but not in cAMP levels ; contraction and the increase in [ Ca2+]i were inhibited by ketanserin . - pubmed
Conversely , in cells with only 5 - HT1p receptors , 5 - HT caused relaxation and an increase in cAMP levels but not in [ Ca2+]i ; relaxation and the increase in cAMP levels were inhibited by 5 - HTP - DP . - pubmed
Thus , the increase in [ Ca2+]i was augmented 1 ) when cAMP production was inhibited by 5 - HTP - DP in naive cells or 2 ) when cAMP production was suppressed in cells where 5 - HT1p receptors were inactivated and only 5 - HT2 receptors were preserved . - pubmed
The results imply that the increase in cAMP levels mediated by 5 - HT1p receptors acted to attenuate the increase in [ Ca2 +]i mediated by 5 - HT2 receptors . - pubmed
alpha - hANP was biotinylated using N - hydroxysuccinimidobiotin and trapped onto an anti - alpha - hANP [ 6 - 28 ] IgG - coated polystyrene ball . - pubmed
The complex formed was trapped onto ( anti-2,4 - dinitrophenyl group ) IgG - coated polystyrene balls and , after washing , reacted with avidin - beta - D - galactosidase conjugate . - pubmed
The polystyrene balls were washed , and the complex of the three components was eluted with epsilon N-2 , 4 - dinitrophenyl - L - lysine and transferred to anti - fluorescein IgG - coated polystyrene balls . - pubmed
beta - D - Galactosidase activity bound to the last polystyrene balls was assayed by fluorometry . - pubmed
Only 12 - 26 % of [ 3H ] thymidine influx occurred through this transporter , the remainder entering the cells through a thymine / uracil - specific transporter described previously . - pubmed
However , NADH formation as a result of hydrogenase interaction with anion - radicals ( CO2.- ) formed in the course of formate photooxidation can not at present be excluded . - pubmed
Peripheral blood lymphocytes taken during hospitalization with IAR therapy ( first half and latter half ) , and every 3 to 6 months for 2 years at the longest after IAR therapy were double - stained with FITC- and PI - labelled antibodies and two - color analysis was conducted by a FACS Analyzer . - pubmed
Thus we conclude that RP 67580 , a SP antagonist , belongs to a class of drugs that may be useful in the management of various clinical pathologies where pain and neurogenic inflammation are involved . - pubmed
The pentapeptide , Tyr - Ile - Gly - Ser - Arg , ( YIGSR ) , an amino acid sequence from the B1 chain of laminin , was found to compete with laminin for binding to the laminin receptor . - pubmed
Progesterone receptor was measured in 97 breast cancer biopsy samples with two methods , the multiple point dextran coated charcoal method with Scatchard analysis and enzyme immunoassay . - pubmed
One obvious advantage with enzyme immunoassay compared to the dextran coated charcoal method is that significantly less tissue is needed . - pubmed
Arterial thromboxane B2 and platelet factor 4 concentrations were elevated on cardiopulmonary bypass in all groups . - pubmed
Whereas 1 microgram / ml of compound 48 / 80 slightly stimulated Ca2 + -dependent phospholipase A2 , higher concentrations led to dose - dependent inhibition of this platelet enzyme . - pubmed
These results demonstrate that the inhibition of platelet aggregation by compound 48 / 80 is not due solely to effects on calmodulin as previously reported , but that inhibition of phospholipases and probably arachidonate mobilization may also be involved . - pubmed
Urine IR - EGF ( U - EGF ) increased about 100 - fold relative to urea concentration and about 1000 - fold relative to urine volume from the 1st day of life to adulthood . - pubmed
The participation of extracellular calcium in transmission of activation into the cell being excluded , the Ca2 + -activation of the contractile apparatus and kinase - phosphorylase system in electrically stimulated muscles presumably proceeds from the induction of Ca2 + release from sarcoplasmic reticulum ( SR ) by transmembrane Na +- or Li+-current . - pubmed
The mechanism of inhibition of phosphoenolpyruvate carboxylase by quinolinic acid . - pubmed
Following the C - banding procedure it was possible to differentially stain the centromeric area with Feulgen and GCA and even with non - fully specific stain for DNA such as methylene blue . - pubmed
Lysine residues in the C - terminal lobe and lysosomal targeting of procathepsin D. - pubmed
A major pathway to the lysosome for soluble hydrolases involves the 6 - phosphorylation of mannose residues . - pubmed
BCECF labeling of cells indicated that the parent and variant cells possess an amiloride sensitive Na +/ H + antiporter of comparable activity . - pubmed
Exposure of parent cells to PGF2 alpha or TPA resulted in the activation of Na +/ H + antiporter activity . - pubmed
These studies strongly suggest that PKC mediated activation of the Na +/ H + antiporter accounts for the inhibition of ammonia production produced by both PGF2 alpha and TPA . - pubmed
Interleukin 1 beta converting enzyme ( ICE ) is responsible for processing an inactive 31 - kDa precursor to the active , mature 17 - kDa Il-1 beta with cleavage occurring between the Asp116 - Ala117 amide bond . - pubmed
Upon cleavage of DABCYL - Tyr - Val - Ala - Asp - Ala - Pro - Val - EDANS ( DABCYL - ICE - EDANS ) , an increase in fluorescence is observed at the EDANS emission wavelength of 490 nm , permitting a continuous assay of ICE that is useful in the screening of inhibitory compounds . - pubmed
A partial prevention of muscle waste was observed with acetylsalicylic acid , a non - steroidal anti - inflammatory drug , or with leupeptin , a proteinase inhibitor . - pubmed
Although treatment with insulin decreased plasma corticosterone to normal values , it did not modify the circulating level of tumour necrosis factor . - pubmed
Histone H4 , at concentrations up to 11.8 microM , increased 2 - deoxyglucose ( 2 - DG ) uptake in a dose - dependent fashion . - pubmed
However , at concentrations higher than 11.8 microM , H4 caused a decrease in 2 - DG uptake from the maximum , suggesting a secondary inhibitory action of this compound . - pubmed
The maximal effect of H4 on 2 - DG uptake was not additive to the maximal effect of insulin . - pubmed
Moreover , 2 - DG uptake in the presence of both H4 and insulin was significantly lower than the 2 - DG uptake in the presence of insulin alone . - pubmed
The maximal effect of H4 on stimulation of 2 - DG uptake was neither additive nor inhibitory to the maximal effects of the intracellularly acting insulin mimetics sodium vanadate or H2O2 . - pubmed
Also , in contrast with the effects of H4 on insulin - stimulated 2 - DG uptake , H4 did not inhibit insulin - like growth factor - I ( IGF - I)-stimulated 2 - DG uptake , as the maximal effects of H4 and IGF - I were additive . - pubmed
These data suggest that H4 interacts with the insulin , rather than the hypoxia / contraction , pathway for activation of glucose transport in muscle tissue , and that H4 acts either directly or indirectly to increase the number of insulin receptors at the surface of the muscle cell . - pubmed
In trabeculae with intact endothelial cells , cAMP increased actomyosin ATPase activity toward an apparently maximum value . - pubmed
In trabeculae with damaged endothelial cells , cAMP did not change actomyosin ATPase activity . - pubmed
The extent to which the force of the isolated trabecula is changed by the coronary venous effluent is closely related to the degree to which cAMP has up - regulated the actomyosin ATPase activity in the isolated heart donating the coronary effluent : the greater the degree of up - regulation of ATPase activity , the greater the increase in force produced by the effluent . - pubmed
The rates of desorption of the NBD - phospholipids from phosphatidylcholine ( PC ) donor vesicles was in the order phosphatidylcholine ( PC ) & gt ; phosphatidylserine ( PS ) & gt ; phosphatidylethanolamine ( PE ) , the slower rates in the PS and PE reflecting direct interlipid hydrogen bonding . - pubmed
Ethanol also increased the rate of NBD - phospholipid desorption from donor PC vesicles in the order PC & gt ; PS & gt ; PE , showing that PC interactions , here limited to the weaker hydrogen - bonded water molecule network , were more susceptible compared to stronger , direct interlipid hydrogen bonds involving PE and PS . - pubmed
We found that induced sputum from asthmatic subjects had a higher percentage of eosinophils [ 8.1 + /- 3.43 ( mean + /- SEM ) versus 0.03 + /- 0.02 % , p & lt ; 0.009 ] ( after excluding squamous cells ) and also had higher levels of albumin ( 232.3 + /- 54.8 versus 79.5 + /- 9.7 micrograms / ml , p & lt ; 0.02 ) , fibrinogen ( 44.2 + /- 11.6 versus 11.9 + /- 2.5 micrograms / ml , p & lt ; 0.008 ) and eosinophil cationic protein ( ECP ) ( 142.6 + /- 34.2 versus 26.1 + /- 4.7 ng / ml , p & lt ; 0.006 ) but not of histamine or tryptase . - pubmed
Characterization of the secondary structure of the antitumor antibiotic C-1027 has been made from a comparison of C-1027 and its apoprotein by various analytical means . - pubmed
The results indicated the antibiotic to be abundant in beta - structure by measurements of Fourier - transform infrared ( FT - IR ) spectroscopy and the circular dichroism ( CD ) spectrum , and by a prediction of the secondary structure based on the amino acid sequence of the peptide . - pubmed
In comparison of the IR spectra of their proteins in D2O , the apoprotein exhibited a faster H - D exchange than C-1027 , indicating an increase in the " non - motile parts " of the beta - sheets formed through the protein - chromophore interaction in holo - C-1027 . - pubmed
Partial amino acid sequence analysis of this protein indicated that these components involved a heterogeneously modified single polypeptide , and that this antigen is identical to human prostatic acid phosphatase . - pubmed
Glucokinase ( GK ) plays a key role in the regulation of glucose - induced insulin secretion , and questions have been raised about its relationship to the glucose transporter GLUT2 and its function in diabetes . - pubmed
The translocation of this key enzyme for glucose recognition by beta - cells may lead to altered rates of insulin secretion during acute perturbations of fuel provision and in the diabetic state . - pubmed
A standardized hyperthyroid symptom score ( HSS ) and thyroid function tests including thyroid - stimulating hormone ( TSH ) , free thyroxine ( free T4 ) , free triiodothyronine ( free T3 ) , total thyroxine ( T4 ) and total triiodothyronine ( T3 ) were evaluated before and after 10 and 30 days of the study period . - pubmed
HSS decreased from 27.80 + /- 4.54 to 22.51 + /- 4.04 after 10 days of diltiazem therapy in Group 1 ( p & lt ; 0.01 ) . - pubmed
The cobalt ( III )-insulin hexamer is a prolonged - acting insulin prodrug . - pubmed
The insulin hexamer has two high - affinity metal ion binding sites , each involving three HisB10 residues , one from each dimer . - pubmed
Insulin hexamers containing Co2 + at both these sites were oxidized to form a stable Co ( 3 +)-insulin complex . - pubmed
It is shown that the Co ( 3 +)-coordinated insulin monomers are released extremely slowly in aqueous solution at pH 8.0 , and that the hexamer does not spontaneously dissociate into subunits at nanomolar concentrations of insulin . - pubmed
The Co ( 3 +)-insulin hexamer is not recognized by the insulin receptor in vitro but the complex shows a protracted action profile following subcutaneous ( s.c . ) - pubmed
The Co ( 3 +)-insulin hexamer provides a novel prodrug approach to a soluble , prolonged - acting insulin preparation of potential use for basal insulin delivery in the treatment of diabetes . - pubmed
Hypertension risk increased with age , heart rate , serum triglycerides , insulin area , and obesity ( in young participants ) . - pubmed
The 5 ' regulatory region of ORF1 contains early ( CGTGC ) and late ( TAAG ) transcriptional initiation motifs and ORF1 is predicted to encode a protein with 114 amino acid residues . - pubmed
ORF4 is included in ORF6 - part and can encode a 48 amino acid residues polypeptide , but ORF4 and ORF6 - part are located on different DNA strands . - pubmed
Dysfunction of nitric oxide induces protein kinase C activation resulting in vasospasm after subarachnoid hemorrhage . - pubmed
We hypothesize that the interaction between protein kinase C ( PKC ) and nitric oxide ( NO ) plays a role in the modulation of cerebral vascular tone , and the disturbance of this interaction following subarachnoid hemorrhage ( SAH ) results in vasospasm . - pubmed
N omega - nitro - L arginine ( L - NA ) , an inhibitor of NO production , enhanced PKC activity . - pubmed
PKC activity in the SAH was significantly higher than in the control ; however , no further enhancement was produced with L - NA . - pubmed
In the SAH , PKC activity was not inhibited either by 8 - bromo - cGMP or SIN-1 . - pubmed
We conclude that NO maintains an appropriate vascular tone through inactivation of PKC , and that this effect is disturbed following SAH , resulting in PKC - dependent vascular contraction , such as vasospasm . - pubmed
On the other hand , once PKC has been activated , NO precursors do not inhibit PKC . - pubmed
These facts indicate NO inactivates PKC through the inhibition of phosphatidylinositol breakdown . - pubmed
ISO , with and without NP , increased cyclic AMP ( from 287 + /- 21 to 477 + /- 33 pmol / g ) without altering cyclic GMP . - pubmed
Left - ventricular pressure increased from 97 + /- 12 to 178 + /- 9 mm Hg and dP / dtmax from 1,786 + /- 275 to 4,049 + /- 354 mm Hg / s. NP increased cyclic GMP ( from 4 to 30 pmol / g ) in both the absence and presence of ISO , but NP did not alter cyclic AMP . - pubmed
Without ISO , NP insignificantly altered left - ventricular pressure ; however , in the presence of ISO , NP significantly decreased left - ventricular pressure by -25 + /- 4 mm Hg and decreased dP / dtmax by -619 + /- 142 mm Hg / s. Isoproterenol increased O2 consumption , but the changes with NP were not significant . - pubmed
When this study was repeated in the presence of LY83583 , a guanylate cyclase inhibitor , NP still produced cardiac depression in the presence of ISO . - pubmed
Therefore , cardiodepressant effects of NP were only observed against a background of inotropic stimulation with ISO . - pubmed
However , effects of NP on contractility were unrelated to increases in cyclic GMP or cyclic GMP - induced changes in cyclic AMP . - pubmed
Pretreatment of RAEC with db - cAMP ( 1 mM ) attenuated Exo A induced cytotoxicity . - pubmed
G protein coupled receptors for prostaglandins are activated when agonists are bound to a binding pocket formed in part by the seven transmembrane domains . - pubmed
Recent studies have determined that substitution of a conserved threonine in the second extracellular loop of the prostaglandin EP3 receptor resulted in increased affinity for ligands with a C1 methyl ester moiety . - pubmed
The homologous threonine in the second extracellular loop of the human prostaglandin EP2 and EP4 receptors was mutated to alanine . - pubmed
In contrast , EP2 and EP4 receptors bearing conservative threonine to serine mutations ( EP2T185S ; EP4T168S ) displayed Kd values for [ 3H ] prostaglandin E2 similar to wild type receptors : 8.8 + /- 0.7 nM for EP2T185S compared to 12.9 + /- 1.2 nM for EP2 wild type ; 2.0 + /- 0.8 nM for EP4T168S compared to 0.9 + /- 0.3 nM for the EP4 wild type receptor . - pubmed
The EC50 values for cAMP stimulation were 1.3 + /- 0.6 nM for EP2 wild type ; 2.7 + /- 1.3 nM for EP2T185S ; 1.1 + /- 0.3 nM for EP4 wild type ; and 1.4 + /- 0.33 nM for EP4T168S. - pubmed
These studies suggest a critical role for the hydroxyl moiety on these conserved threonine residues at position 168/185 of the second extracellular loop in prostaglandin receptor - ligand interactions . - pubmed
A randomized prospective comparison of oral levofloxacin plus intraperitoneal ( IP ) vancomycin and IP netromycin plus IP vancomycin as primary treatment of peritonitis complicating CAPD . - pubmed
OBJECTIVE : To compare the therapeutic efficacy of daily oral levofloxacin plus intermittent intraperitoneal ( IP ) vancomycin ( group 1 ) versus daily IP netromycin and intermittent IP vancomycin ( group 2 ) in the primary treatment of peritonitis complicating continuous ambulatory peritoneal dialysis ( CAPD ) . - pubmed
CONCLUSION : Oral levofloxacin in combination with intermittent IP vancomycin has comparable efficacy to IP netromycin combined with intermittent IP vancomycin as primary treatment in CAPD peritonitis , but is simpler and more cost - effective to administer . - pubmed
Although treatment with donepezil did not normalize EP latencies , treatment was associated with a significant decrease in the auditory P300 latency ( mean latency pretreatment=401 . - pubmed
Treatment with donepezil had no discernible effect on auditory or visual P300 EP amplitudes . - pubmed
As expected , the particle free energy is higher in the right - handed state ( DeltaG(t)=1.9(+/-0.I ) kT ) , but it decreased ( to 1.3(+/-0.1 ) kT ) upon histone acetylation and the addition of phosphate , a potent tail destabilizer . - pubmed
Eukaryotic translation initiation factor 5A ( eIF-5A ) is the only cell protein that contains the unusual basic amino acid hypusine [ N epsilon -( 4 - amino-2 - hydroxybutyl ) lysine ] . - pubmed
Hypusine is formed by the transfer of the butylamine portion from spermidine to the epsilon - amino group of a specific lysine residue of eIF-5A precursor and the subsequent hydroxylation at carbon 2 of the incoming 4 - aminobutyl moiety . - pubmed
Here we have studied whether the inhibition of hypusine synthesis can potentiate the anti - cancer activity of the anti - tumour agents interferon - alpha ( IFN alpha ) and cytosine arabinoside ( ara - C ) . - pubmed
Growth inhibition induced by IFN alpha was paralleled by decreased hypusine synthesis and , when EGF counteracted anti - proliferative effects , a reconstitution of hypusine levels was recorded . - pubmed
UNLABELLED : Calcium chloride ( CaCl ( 2 ) ) is ineffective in severe calcium channel antagonist overdoses . - pubmed
Digoxin increases intracellular calcium by inhibiting the sodium - potassium adenosine triphosphatase enzymes . - pubmed
Mutation of Cys-67 alters the thermodynamic stability of the human leukocyte antigen HLA0 - B * 2705 . - pubmed
Among the four heavy chain mutations , the C67S change was investigated for unfolding and peptide binding properties because this position may mediate disulfide pair bridging and alter T - cell recognition of HLA - B * 2705 . - pubmed
Cys-67 seems to play a crucial role in controlling the thermodynamic stability of the B * 2705 - peptide complexes as the C67S mutant unfolds faster and with a single transition , independent of pH. - pubmed
Bradykinin - induced increase in the intracellular concentration of free calcium evokes an activation of the endothelial nitric oxide synthase ( eNOS ) enzyme , producing nitric oxide ( NO ) . - pubmed
Cigarette smoke inhibits the eNOS - NO - cGMP signaling pathway . - pubmed
After the purification with DEAE ion - exchange chromatography , HSP70 with a purity of higher than 90 % was obtained . - pubmed
Activation of hydrogen peroxide in horseradish peroxidase occurs within approximately 200 micro s observed by a new freeze - quench device . - pubmed
To observe the formation process of compound I in horseradish peroxidase ( HRP ) , we developed a new freeze - quench device with approximately 200 micro s of the mixing - to - freezing time interval and observed the reaction between HRP and hydrogen peroxide ( H ( 2 ) O ( 2 ) ) . - pubmed
We conclude that the activation of H ( 2 ) O ( 2 ) in HRP at ambient temperature completes within approximately 200 micro s. The developed device can be generally applied to investigate the electronic structures of short - lived intermediates of metalloenzymes . - pubmed
Immunohistochemical staining of cells using isoform - specific antibodies further demonstrated translocation of the phorbol ester - sensitive isoforms , PKCalpha and PKCepsilon , to both the plasma membrane and perinuclear sites , reflecting potential initial steps in regulation of specific effector pathways . - pubmed
Indeed , selective inhibition of PKCs indicates a potential role for PKCalpha in modulating a calcium influx channel . - pubmed
The in vitro and in vivo enantioselectivity of etomidate implicates the GABAA receptor in general anaesthesia . - pubmed
In electrophysiological and radioligand binding assays , the enantiomers of etomidate positively regulated GABAA receptor function at anaesthetic concentrations and with an enantioselectivity paralleling their in vivo activity . - pubmed
GABA - evoked currents mediated by human recombinant GABAA receptors were potentiated by either R -(+)- or S -(-)- etomidate in a manner dependent upon receptor subunit composition . - pubmed
Modulation of GABA receptor activity was selective ; R -(+)- etomidate inhibited nicotinic acetylcholine , or 5 - hydroxytryptamine3 receptor subtypes only at supra - clinical concentrations and ionotropic glutamate receptor isoforms were essentially unaffected . - pubmed
Collectively , these observations strongly implicate the GABAA receptor as a molecular target relevant to the anaesthetic action of etomidate . - pubmed
The protein encoded by one of the clones interacting with neuroglobin ( NGB ) was confirmed to be the C terminus of the Na (+ ) , K (+)- ATPase beta2 subunit ( NKA1b2 ) based on amino acid sequences . - pubmed
We tested whether dexamethasone ( DEXA ) administration influences the proliferation , apoptosis , and number of B- and T - lymphocytes in Peyer 's patches ( PP ) and thymus in calves fed colostrum ( C ) or a milk - derived formula . - pubmed
The DEXA ( 30 microg / kg of BW daily ) was injected for 4 d into groups fed colostrum on the first 3 d ( CD+ ) and those fed the formula that contained nutrients in amounts as in colostrum but no immunoglubulin G ( FD+ ) . - pubmed
There were significant effects ( P & lt ; 0.05 ) of DEXA treatment ( decrease of cell proliferation rates in follicles of PP and thymus , increase of apoptotic rate in follicles of PP and thymus , decrease of B - lymphocyte numbers in follicles of PP , increase of B - lymphocyte numbers in domes of PP , increase of T - lymphocyte numbers in follicles of PP , and a decrease of intraepithelial T - lymphocyte numbers ) . - pubmed
In conclusion , DEXA treatment decreased cell proliferation rates in follicles of PP and thymus and enhanced apoptotic rates in follicles of PP . - pubmed
Colostrum feeding decreased cell proliferation rates , likely of B - lymphocytes , in follicles of PP and numbers of B - lymphocytes in domes , follicular - associated epithelium , and interfollicular areas of PP and enhanced cell proliferation rates and selectively modified DEXA effects in the thymus . - pubmed
THE MEASUREMENT OF FIBRINOLYSIS WITH RADIOACTIVE MERCURY LABELLED FIBRIN CLOTS . - pubmed
[ The treatment of the Parkinson syndrome with disipal ( BS 5930 ) ] . - pubmed
METHODS : We retrospectively evaluated hazardous concomitant prescriptions of hydroxymethylglutaryl coenzyme A reductase inhibitors ( statins ) and azole antifungals , which were invoiced through 9 months of 2001 within an outpatient setting . - pubmed
The euryarchaeal nitrogen repressor NrpR controls the expression of the nif ( nitrogen fixation ) operon , resulting in full repression with ammonia , intermediate repression with alanine , and derepression with dinitrogen . - pubmed
Here we have undertaken both in vivo and in vitro approaches to study the way in which NrpR , nifOR ( 1 ) , nifOR ( 2 ) , and the effector 2 - oxoglutarate ( 2OG ) combine to regulate nif expression , leading to a comprehensive understanding of this archaeal regulatory system . - pubmed
nifOR ( 1 ) has stronger binding and by itself can mediate the repression of nif transcription during growth on ammonia , unlike the weakly binding nifOR(2 ) . - pubmed
However , nifOR(2 ) in combination with nifOR(1 ) is critical for intermediate repression during growth on alanine . - pubmed
NrpR responds directly to 2OG , which weakens its binding to the operators . - pubmed
Hence , 2OG is an intracellular indicator of nitrogen deficiency and acts as an inducer of nif transcription via NrpR. - pubmed
We studied single - nucleotide polymorphisms in transforming growth factor ( TGF )- beta1 , interleukin ( IL )- 10 , IL-6 , interferon ( IFN )- gamma and tumour necrosis factor ( TNF)-alpha genes in the Finnish population using family - based association approach . - pubmed
To validate the method , three contrast agents targeted to integrins were synthesized by grafting to USPIO ( ultrasmall particles of iron oxide ): ( a ) the CS1 ( connecting segment-1 ) fragment of fibronectin ( FN ) ( USPIO - g - FN ) ; ( b ) the peptide GRGD ( USPIO - g - GRGD ) ; ( c ) a non - peptidic RGD mimetic ( USPIO - g - mimRGD ) . - pubmed
Delta R2 was converted in values of iron concentration based on a calibration curve . - pubmed
Low - density lipoprotein - cholesterol , lipoprotein ( a ) , and hemoglobinA1C levels were determined . - pubmed
NM-3 significantly suppressed the increase of VEGF induced by high glucose in cultured podocytes and also suppressed the increase of VEGF and TGF - beta induced by high glucose in cultured mesangial cells . - pubmed
Barth syndrome ( BTHS ) is a mitochondrial disorder that is caused by mutations in the tafazzin gene , which affects phospholipid composition . - pubmed
G - protein beta-3 subunit C825 T polymorphism , sodium and arterial blood pressure : a community - based study of Japanese men and women . - pubmed
Epidemiological evidence on gene - environment effects of the G - protein beta-3 subunit C825 T polymorphisms and sodium on blood pressure in the free - living general population is limited . - pubmed
Our results indicate that the G - protein beta-3 subunit C825 T polymorphism is associated with higher systolic blood pressure levels in a large free - living Japanese population , and more specifically in women with a low sodium intake . - pubmed
Relative quantification of tau - related peptides using guanidino - labeling derivatization ( GLaD ) with online - LC on a hybrid ion trap ( IT ) time - of - flight ( ToF ) mass spectrometer . - pubmed
By in situ dye labeling , we show that neonatal and adult SVZ progenitors differentially contribute to neurochemically distinct types of periglomerular interneurons in the GL . - pubmed
Deletions of the sFRP-1 cysteine rich domain ( CRD ) lead to the complete loss of Wnt antagonist function . - pubmed
A region between amino acids 73 - 86 within the second loop of the CRD of sFRP-1 was necessary for the optimal Wnt inhibitory function . - pubmed
Within this region , a conserved tyrosine residue played a critical role , and its change to neutral or polar amino acids lead to decreased Wnt inhibitory activity . - pubmed
The sFRP-1 / sFRP-2 chimera with the replacement of C - terminal 19 amino acids of sFRP-1 with 11 amino acids of sFRP-2 resulted in 70 % loss of activity indicating that carboxyl - terminal region of sFRP-1 is important for its Wnt inhibitory activity . - pubmed
Samples were removed at predefined time points and analysed for acetylsalicylic acid ( ASA ) and salicylic acid ( SA ) content using stability - indicating HPLC . - pubmed
LTD was mimicked and occluded by mGluR agonists and blocked by nonselective mGluR antagonist ( RS )- alpha - methyl-4 - sulfonophenylglycine ( MSPG ) but not by N - methyl - D - aspartic acid ( NMDA ) receptor antagonist 2 - amino-5 - phosphonovaleric acid ( APV ) . - pubmed
AIM : Radiation exposure to the kidney limits therapy with radiometal labelled DOTATOC . - pubmed
Hormones like ACTH , corticosteroids and catecholamines as well as cytokines , chemokines and alarmins play important roles in the initiation and persistence of the pro - inflammatory response after severe injury . - pubmed
Ca2 + entry through store - operated Ca2 + channels drives the production of the pro - inflammatory molecule leukotriene C4 ( LTC4 ) from mast cells through a pathway involving Ca2 + - dependent protein kinase C , mitogen - activated protein kinases ERK1 / 2 , phospholipase A2 , and 5 - lipoxygenase . - pubmed
Manipulating the amplitude and spatial extent of Ca2 + entry by altering chemical and electrical gradients for Ca2 + influx or changing the Ca2 + buffering of the cytoplasm all impacted on protein kinase C and ERK activation , generation of arachidonic acid and LTC4 secretion , with little change in the bulk cytoplasmic Ca2 + rise . - pubmed
Similar bulk cytoplasmic Ca2 + concentrations were achieved when CRAC channels were activated in 0.25 mm external Ca2 + versus 2 mm Ca2 + and 100 nm La3 + , an inhibitor of CRAC channels . - pubmed
However , despite similar bulk cytoplasmic Ca2 + , protein kinase C activation and LTC4 secretion were larger in 2 mm Ca2 + and La3 + than in 0.25 mm Ca2 + , consistent with the central involvement of a subplasmalemmal Ca2 + rise . - pubmed
Recombinant TRPC3 channels also activated protein kinase C , suggesting that subplasmalemmal Ca2 + rather than a microdomain exclusive to CRAC channels is the trigger . - pubmed
Enterostatin inhibition of angiogenesis : possible role of pAMPK and vascular endothelial growth factor A ( VEGF - A ) . - pubmed
METHOD : To investigate this hypothesis , we studied the effect of enterostatin as an antiangiogenic agent in two angiogenic tissue culture model systems . - pubmed
The AMPK activator 5 - aminoimidazole-4 - carboximide ribonucleoside ( AICAR ) induced VEGF - A expression . - pubmed
The resemblance suggests that L1CAM may be a target for ethanol , and consistent with this idea , ethanol can inhibit L1CAM adhesion in cell lines and L1CAM - mediated outgrowth and signaling in cerebellar granule neurons . - pubmed
However , it is not known whether ethanol inhibits L1CAM function in other neuron types known to require L1CAM for appropriate development . - pubmed
We find that ethanol does not alter axonal polarization , L1CAM - dependent axon outgrowth or branching , or L1CAM recycling in axonal growth cones . - pubmed
Thus , ethanol inhibition of L1CAM is highly dependent on neuronal context . - pubmed
Free copper at baseline correlated with the ADL and IADL clinical scales scores at 1 year . - pubmed
Site - specific mono - PEGylations were performed in different conformational regions of Thymosin alpha 1 ( T alpha 1 ) by introducing one cysteine residue into the chosen site and coupling with thiol - specific mPEG - MAL reagent . - pubmed
Treatment of antisense c - raf.1 oligomers enhanced cytochrome P-450 content ( 81 % ) and reduced glutathione S - transferase activity ( 33 % ) , UDP glucuronosyltransferase activity ( 74 % ) and MDA concentration ( 30 % ) in carcinogen and antisense oligomer - treated group as compared with carcinogen control animals . - pubmed
The SFs were grown on a commercially available collagen mix that was placed onto the upper side of a Transwell polycarbonate membrane . - pubmed
BACKGROUND : Despite increasing evidence for the presence of voltage - gated Na(+ ) channels ( Na ( v ) ) isoforms and measurements of Na ( v ) channel currents with the patch - clamp technique in arterial myocytes , no information is available to date as to whether or not Na ( v ) channels play a functional role in arteries . - pubmed
In parallel , using real time RT - PCR , we identified three Na ( v ) transcripts : Na(v)1.2 , Na(v)1.3 , and Na(v)1.5 . - pubmed
KB - R7943 , an inhibitor of the reverse mode of the Na (+)/ Ca ( 2 + ) exchanger , mimicked the effect of TTX and had no additive effect in presence of TTX . - pubmed
CONCLUSIONS / SIGNIFICANCE : These results define a new role for Na ( v ) channels in arterial physiology , and suggest that the TTX - sensitive Na ( v ) 1.2 isoform , together with the Na (+)/ Ca ( 2 + ) exchanger , contributes to the contractile response of aortic myocytes at physiological range of membrane depolarization . - pubmed
The distribution of PAHs and PCDD / Fs in different MSWI unit ashes , including bottom residue ( BR ) , fly ash pit ( FAP ) , fabric filter ( FF ) , semi - dryer absorber ( SDA ) , economizer ( EC ) and super heater ( SH ) , has seldom been discussed . - pubmed
Average PAHs content in ash samples from BR , FAP , FF , SDA , EC and SH were found to be 0.020 , 0.237 , 0.234 , 0.125 , 0.037 and 0.034 microg TEQ g(-1 ) , respectively . - pubmed
Furthermore , PAHs in ashes were mainly found in BR ( 41 % ) and FAP ( 31 % ) . - pubmed
The FAP ash included 18 % of PAHs coming from FF ash . - pubmed
On the other hand , PCDD / Fs content in BR , FAP , FF , SDA , EC and SH was found to be 0.0329 , 1.1589 , 1.2807 , 0.1476 , 0.6868 and 0.0921 ng I - TEQ g(-1 ) , respectively . - pubmed
The FAP ash included 33 % of PCDD / Fs coming from FF ash . - pubmed
Therefore , PCDD / Fs content in FAP and FF exceeded the legal limit of 1 ng I - TEQ g ( -1 ) ; therefore , a solidification process is necessary before landfilling in Taiwan . - pubmed
Treatment usually consists of carvedilol and an angiotensin - converting enzyme inhibitor or angiotensin II receptor blocking agent if left ventricular ejection fraction is less than 40 % . - pubmed
Next , Experimental test 10H2DA how to inhibited the activities of MMPs : with RASFs isolated from rheumatoid tissues by enzymatic digestion , cultures in monolayers were treated with 10H2DA ( 0.5mM , 1mM , and 2mM ) or PBS for 2h followed by stimulation with TNF - alpha ( 10 ng / ml ) for 2h , mRNA . - pubmed
RESULTS : The molecular investigation revealed that the 10H2DA - mediated suppression was likely to occur through blocking p38 kinase and c - Jun N - terminal kinase - AP-1 signaling pathways . - pubmed
In contrast , 10H2DA had no effect on extracellular signal - regulated kinase activity , NF - kappaB DNA - binding activity and IkappaBalpha degradation . - pubmed
The anti - CD20 monoclonal antibody rituximab is an effective treatment for small lymphocytic lymphoma ; however , it has been associated with infusion reactions , including cardiac arrhythmias . - pubmed
Amino acid starvation by asparaginase ( ASNase ) enhances phosphorylation of eukaryotic initiation factor 2 ( eIF2 ) by general control nonderepressible 2 ( GCN2 ) kinase , leading to reduced global mRNA translation rates . - pubmed
This study addressed the importance of GCN2 for the immune system to adapt to amino acid starvation by ASNase . - pubmed
These results indicate that loss of GCN2 enhances immunosuppression by ASNase and that this eIF2 kinase is broadly required for amino acid stress management in the immune system . - pubmed
Dimerization and its role in GMP formation by human guanylate binding proteins . - pubmed
Human guanylate binding proteins ( hGBP-1 and 2 ) belonging to large GTPases have the unique feature of hydrolyzing GTP to a mixture of GDP and GMP with unequal ratios . - pubmed
Remarkably , the enzyme exhibits differential GTPase activities depending on dimerization ; a monomer produces only GDP , but a dimer gives both GDP and GMP with stimulation of the activity . - pubmed
Similar to hGBP-1 , hGBP-2 showed dimerization - related GTPase activity for GMP formation , indicating that this family of proteins follows a broadly similar mechanism for GTP hydrolysis . - pubmed
The obtained copper ( I ) complexes were characterized in detail by time - dependent 1D [ ( 1 ) H , ( 13 ) C ] and 2D [ ( 1 ) H - NOESY ] NMR spectroscopy , elemental analysis , high - resolution ESI - TOF mass spectrometry , and analytical ultracentrifugation . - pubmed
CONCLUSION : These findings indicate that alterations in high - mannose type N - glycans and N - glycogenes in chondrocytes correlate with the release of MMP-13 and ADAMTS-5 during cartilage degradation . - pubmed
dexamethasone , preoperative administration of analgesia including the use of non - steroidal anti - inflammatory or COX II inhibitors , intraoperative use of an anti - emetic , pre - incisional use of bupivacaine , administration of intraperitoneal bupivacaine on establishment of pneumoperitoneum , and avoidance of drains . - pubmed
For the OPA1 gene , the exons were amplified by PCR and mutations were detected by restriction enzymes or the dye terminator method . - pubmed
Displacement was defined as the length between the center of gravity of titanium markers and the needle applicator tips along the daily CT axis . - pubmed
Inflow and outflow samples were collected and analysed for Total Suspended Solids ( TSS ) , Total Phosphorus ( TP ) and Total Nitrogen ( TN ) . - pubmed
Histone deacetylase-2 ( HDAC-2 ) plays a pivotal role in many cases of steroid insensitivity . - pubmed
The main aim of this study was to restore the smoking - induced reduction in corticosteroid sensitivity by increasing HDAC-2 activity using low - dose theophylline . - pubmed
These results indicate that low - dose theophylline might provide protection from smoke damage and improve the anti - inflammatory effects of steroids by increasing HDAC-2 activity . - pubmed
A conserved N - terminal arginine - motif in GOLPH3 - family proteins mediates binding to coatomer . - pubmed
We identify a cluster of arginine residues at the N - terminal end of GOLPH3 proteins that are necessary and sufficient to mediate coatomer binding . - pubmed
We establish that the oligomerization status and phosphatidylinositol-4 - phosphate - binding properties of Vps74p largely account for the membrane - binding capacity of the protein and identify an Arf1p - Vps74p interaction as a potential contributing factor in Vps74p Golgi membrane association . - pubmed
To ascertain whether the defect in tdy2 leaves affected the movement of sucrose into the phloem or its long - distance transport , we performed radiolabeled and fluorescent dye tracer assays . - pubmed
The disruption of sucrose movement in the phloem in tdy2 mutants provides evidence that the Tdy2 callose synthase functions in vascular maturation and that the vascular defects result in impaired symplastic trafficking into the phloem translocation stream . - pubmed
Clinical and preclinical data suggest that ZOL may have also anti - angiogenic activity ; however , the effects of Dmab ( a fully humanized antibody against the receptor activator of nuclear factor kappa B ligand ) on angiogenesis are largely unknown . - pubmed
FDG - PET and PET / CT are useful methods to identify malignant change in neurogenic tumors in NF1 and to discriminate malignant from benign neurogenic lesions . - pubmed
Administration of trona significantly reduced ( p & lt ; 0.05 ) alkaline phosphatase activity in the liver and kidney with corresponding increases in the serum enzyme . - pubmed
Blood samples at 0 , 20 , 40 , 60 min of the standard mixed meal were collected to test plasma glucose , serum insulin and C peptide . - pubmed
The area under the curve ( AUC ) of plasma glucose and serum C peptide was significantly lower after the INJEX30 injection than that after insulin pen injection [ plasma glucose AUC ( 542 + - 172)min mmol L(-1 ) vs ( 601 + - 199 ) min mmol L(-1 ) , P & lt ; 0.01 ; C peptide AUC ( 70 + - 53 ) min microg L(-1 ) vs ( 80 + - 58 ) min microg L(-1 ) , P & lt ; 0.01 ] . - pubmed
Phenome - wide association studies on a quantitative trait : application to TPMT enzyme activity and thiopurine therapy in pharmacogenomics . - pubmed
We tested our approach on thiopurine S - methyltransferase ( TPMT ) activity in patients treated by thiopurine drugs . - pubmed
Data after initiation of thiopurine treatment were analyzed and patient groups were compared according to their TPMT activity level . - pubmed
In pretreated NSCLC , EGFR TKIs are considered more effective than standard monotherapy with cytotoxics in presence of classical EGFR mutations , whereas in the EGFR wild - type population , a similar efficacy to docetaxel or pemetrexed in term of survival has been demonstrated . - pubmed
Enteral feeds contributed 16.2 % to overall administered sodium , and were the major source in patients in the PICU for & gt ; 10 days . - pubmed
The mean fasting plasma glucose level at onset of HNF1beta - MODY was considerably higher and the age of onset of HNF1beta - MODY was considerably older than they were for HNF1alpha - MODY , while the mean BMI and C - peptide index at onset were similar . - pubmed
A significant improvement in anti - Toxoplasma effect among quinoline derivatives was detected in B11 , B12 , B23 , and B24 , whose structures carry two quinoline rings , and their resultant cLogP values are & gt;=7 . - pubmed
When treated with quinoline , 8 - hydroxyquinoline or B23 , 40 - 45 % of the parasites lost their apicoplasts . - pubmed
More importantly , B12 and B23 which harbor superior anti - cancer properties than chloroquine , have effective anti - Toxoplasma activity . - pubmed
Per protocol ( PP ) analysis , including only participants who accessed the interventions and also reported accessing additional outside interventions during the trial , showed that about 75 % of extended self - help participants moved from probable dependence , harmful or hazardous use to lower alcohol use levels at 3- and 6- and 12 - month follow - ups , compared to about 40 - 60 % of brief intervention users and controls who accessed extra help ( p & lt ; 0.05 ) . - pubmed
In contrast , PP analysis suggested that cognitive - behavioral extended self - help in combination with other interventions was more effective in changing alcohol use than brief intervention or assessment only . - pubmed
The substrate - binding cleft in SpPth is formed with three loops : the gate loop , Ile91 - Leu102 ; the base loop , Gly108 - Gly115 ; and the lid loop , Gly136 - Gly150 . - pubmed
Effects of melatonin on prenatal dexamethasone - induced epigenetic alterations in hippocampal morphology and reelin and glutamic acid decarboxylase 67 levels . - pubmed
The programming effects of prenatal dexamethasone exposure on the brain were assessed at postnatal days ( PND ) 7 , 42 , and ~120 . - pubmed
At ~PND120 , melatonin restored the reduced levels of hippocampal reln and GAD1 mRNA expression in the DEXM group . - pubmed
In addition , melatonin restored the reln mRNA expression levels by ( 1 ) reducing DNA methyltransferase 1 ( DNMT1 ) mRNA expression and ( 2 ) reducing the binding of DNMT1 and the methyl - CpG binding protein 2 ( MeCP2 ) to the reln promoter . - pubmed
The present study showed that prenatal dexamethasone exposure induced gross alterations in hippocampal morphology and reduced the levels of hippocampal mRNA expression of reln and GAD1 . - pubmed
Thus , melatonin had a beneficial effect in restoring hippocampal reln mRNA expression by reducing DNMT1 and MeCP2 binding to the reln promoter . - pubmed
Previous studies in vanadium toxicity showed involvement of oxidative stress in isolated liver hepatocytes and mitochondria via increasing of ROS formation , release of cytochrome c and ATP depletion after incubation with different concentrations ( 25 - 200 microM ) . - pubmed
Aromatic L - amino acid decarboxylase cells in the spinal cord : a potential origin of monoamines . - pubmed
This compound goes on to display a coil - globule transition above a certain threshold of acetone . - pubmed
Rapamycin is helpful in the treatment of certain cancers by inhibiting mTOR ( mammalian target of rapamycin ) pathway . - pubmed
Western blot assay showed that rapamycin led to release of cytochrome c from mitochondrial membranes to cytosol . - pubmed
Further Western blot assays showed that rapamycin induced activation of caspase-9 and caspase-8 and the cleavage of caspase-3 . - pubmed
Rapamycin induced cleavages of caspase-3 and apoptosis was inhibited by both Z - LETD - FMK and Z - IETD - FMK treatment . - pubmed
Postharvest treatment of ' Gala ' with propylene did not affect the internal ethylene concentration of the fruit but 1 - MCP markedly inhibited it . - pubmed
Bioinformatic , phenotypic , and gene expression analyses demonstrated that the KdgR regulon included genes involved in uptake and metabolism of molecules resulting from pectin degradation as well as those central to the utilization of a number of other carbon sources . - pubmed
Trichloroacetic acid ( TCA ) was used to precipitate the protein and separate the dextran sulfate . - pubmed
Detection and quantitation of dextran sulfate was achieved by post column reaction with dimethylene blue to form a metachromatic complex that absorbs visible light at 530 nm . - pubmed
The enzyme was strongly inactivated by serine protease inhibitor PMSF . - pubmed
The values of Km and Vmax for racemic 2,2 - dimethylcyclopropane carboxamide ( DMCPCA ) were 4.58 mM and 35.03 mumol min(-1 ) mg(-1 ) protein , respectively . - pubmed
The amidase showed a broad substrate spectrum toward aliphatic , aromatic and heterocyclic amides , but could hardly hydrolyze the bulky side - chain - containing amides . - pubmed
Furthermore , kinetic resolution of racemic DMCPCA by the amidase afforded S - DMCPCA in 46.3 % yield and 99 % ee with an average E - value of 67 . - pubmed
These unique properties of the amidase imply that it is a promising biocatalyst for the production of chiral amides and carboxylic acids . - pubmed
A cocktail approach , with dosages of the probe drugs caffeine ( 3.0 mg / kg ) , metoprolol ( 2.33 mg / kg ) and midazolam ( 0.45 mg / kg ) , was used to measure cytochrome P450 ( CYP ) metabolic activities . - pubmed
Tet had no or minor impact on the pharmacokinetics and metabolism of the probe drugs caffeine and metoprolol , CYP1A2 and CYP2D6 substrates , respectively . - pubmed
However , Tet increased AUC0 - 24 h and decreased AUCratio(0 - 24 h ) ( 1 - hydroxymidazolam / midazolam ratio ) for midazolam statistically significant , both in single or multiple dosing of Tet , with up to 39 or 57 % increase for AUC0 - 24 h and 29 % or 22 decrease for AUCratio(0 - 24 h ) , respectively , in line with previous in vitro findings for its CYP3A4 inhibition . - pubmed
The extensive use of Tet and herbal medicines containing Tet makes Tet a candidate for further evaluation of CYP3A - mediated herb - drug interactions . - pubmed
Although VMAT2 is known to package monoamines into synaptic vesicles , in VENs and fork cells its expression occurs in the absence of monoamine - synthesizing enzymes or reuptake transporters . - pubmed
Compared to low LDL cholesterol , high LDL cholesterol was associated with longer RFS in univariable analysis ( continuous : Hazard Ratio ( HR ): 0.67 , 95%-Confidence Interval ( CI ): 0.47 - 0.96 , P = 0.03 ; 3 mM cut - point : HR : 0.44 , 95%-CI : 0.24 - 0.79 , P = 0.006 ) . - pubmed
Preoperative LDL cholesterol remained an independent predictor for PCa recurrence in a multivariable model adjusted for age , preoperative PSA , statin use , tumor stage , Gleason score , nodal status and surgical margin status ( continuous : HR : 0.66 , 95%-CI : 0.44 - 0.99 , P = 0.04 ; 3 mM cut - point : HR : 0.41 , 95%-CI : 0.21 - 0.78 , P = 0.007 ) . - pubmed
Acetate reduction to acetaldehyde and further to ethanol via an aldehyde : ferredoxin oxidoreductase ( AOR ) and alcohol dehydrogenase has been postulated alongside the classic pathway of ethanol formation via a bi - functional aldehyde / alcohol dehydrogenase ( AdhE ) . - pubmed
Here we demonstrate that AOR is critical to ethanol formation in acetogens and inactivation of AdhE led to consistently enhanced autotrophic ethanol production ( up to 180 % ) . - pubmed
The aor1 + 2 double knockout strain lost the ability to convert exogenous acetate , propionate and butyrate into the corresponding alcohols , further highlighting the role of these enzymes in catalyzing the thermodynamically unfavourable reduction of carboxylic acids into alcohols . - pubmed
Several mechanisms have been proposed including altered glucose and fatty acid metabolism , hepatic fatty acid storage , and modulation of glucagon - like peptide ( GLP )- 1 . - pubmed
A rapid and selective method for the quantitation of neurotransmitters , l - Glutamic acid ( GA ) and gamma - Aminobutyric acid ( GABA ) , was developed and validated using gas chromatography - tandem mass spectrometry ( GC - MS / MS ) . - pubmed
The method calibration was linear from 0.5 to 100mug / mL , with limits of detections of 100ng / mL and 250ng / mL for GA and GABA , respectively . - pubmed
Furthermore , these results suggest that loss of Oat1 or Oat3 function in isolation does not result in significant alterations in brain tissue levels of GA or GABA . - pubmed
Expert commentary : In this report , we review the up - to - date findings of more potency roles of silibinin in beta - thalassemia ( beta - TM ) , acute myeloid leukemia ( AML ) , anaplastic large cell lymphoma ( ALCL ) and multiple myelomas ( MM ) therapy and attempt to clarify the mechanisms underlying its effects . - pubmed
Courses were primarily dispensed to men who have sex with men ( 84 % ) and consisted overwhelmingly of a three - drug antiretroviral therapy regimen containing two nucleoside reverse transcriptase inhibitors and either an integrase inhibitor ( raltegravir ) or a boosted protease inhibitor ( lopinavir / ritonavir ) . - pubmed
The deoxyribonucleic acid ( DNA ) repair gene encoding the excision - repair cross - complementation group 1 ( ERCC1 ) protein is known to predict the response to platinum - based chemotherapy . - pubmed
Hepatic superoxide dismutase ( SOD ) and malondialdehyde ( MDA ) were detected . - pubmed
Our results showed marked increase in all measured parameters except SOD , TAC , eNOS immunoexpression and Bcl2 mRNA gene expression which decreased in Cd induced hepatotoxicity group . - pubmed
NEB showed marvelous protective effect against Cd induced changes . - pubmed
NEB decreased liver enzymes ( ALT and AST ) , mean arterial pressure , total cholesterol levels , MDA , iNOS immunoexpression and TNF - alpha gene expression but significantly increased SOD , TAC , eNOS immunoexpression and Bcl-2 gene expression . - pubmed
Moreover ; NEB markedly improved the histopathological changes induced by Cd . - pubmed
These findings prove the antioxidant , anti - apoptotic and anti - inflammatory properties of NEB and its protective role in Cd induced hepatotoxicity . - pubmed
Lipid bilayer - coated mesoporous silica nanoparticles carrying bovine hemoglobin towards an erythrocyte mimic . - pubmed
Hemoglobin ( Hb)-loaded mesoporous silica nanoparticles ( MSNs ) coated with a lipid bilayer ( LB - MSNs ) were investigated as an erythrocyte mimic . - pubmed
Furthermore the encapsulated Hb exhibits similar peroxidase - like activity in catalyzing the oxidation of 2,2 '- azino - bis ( 3 - ethylbenzothiazoline-6 - sulfonic acid ) diammonium salt ( ABTS ) with hydrogen peroxide . - pubmed
The natural course of classical diabetic nephropathy is initially microalbuminuria or moderately increased urine albumin excretion ( 30 - 300mg / g creatinine ) . - pubmed
Multifactorial intervention targeting glucose , lipids and blood pressure including blockade of renin angiotensin system and lifestyle , has improved renal and cardiovascular prognosis and reduced mortality with 50 % . - pubmed
Carboplatin in BRCA1 / 2 - mutated and triple - negative breast cancer BRCAness subgroups : the TNT Trial . - pubmed
HR also repairs DNA lesions caused by platinum agents and PARP inhibitors . - pubmed
Triple - negative breast cancers ( TNBCs ) harbor subpopulations with BRCA1 / 2 mutations , hypothesized to be especially platinum - sensitive . - pubmed
Cancers in putative ' BRCAness ' subgroups - tumors with BRCA1 methylation ; low levels of BRCA1 mRNA ( BRCA1 mRNA - low ) ; or mutational signatures for HR deficiency and those with basal phenotypes - may also be sensitive to platinum . - pubmed
We conclude that patients with advanced TNBC benefit from characterization of BRCA1 / 2 mutations , but not BRCA1 methylation or Myriad HRD analyses , to inform choices on platinum - based chemotherapy . - pubmed
Hypotension , but not the increase in colorectal pressure , was inhibited by a 10 - min pretreatment with the NK1 receptor antagonist CP-99,994 . - pubmed
In a pilot experiment using subcutaneous ( SC ) injection , a similar dose range of LMN - NKA ( 3 - 100 mug / kg ) again appeared to increase colorectal pressure with a similar AUC ( up to 18,546 mmHg*s ) to that seen after IV injection , but lower peak amplitude ( up to 49 mmHg ) . - pubmed
Results : The differences between the groups ' levels of HbA1c ( P=0.02 ) and HDL ( P=0.02 ) were significant , but the differences between their FBS , cholesterol , triglycerides and LDL were not significant ( P>0.05 ) . - pubmed
The reproduction assays gave EC50 and EC10 values , respectively , of 6.3 and 1.4 mg a.s./kg dry soil for abamectin ; 1.0 and 0.12 mg a.s./kg dry soil for Kraft 36 EC ; and 54 and 23 mg a.s./kg dry soil for Score 250 EC . - pubmed
PURPOSE : To compare and evaluate bone regeneration with and without combining platelet rich plasma ( PRP ) , calcium sulfate ( CS ) and autogenous bone graft in periapical defects of jaw . - pubmed
In the first group ( study group ) the bone defect was filled with PRP , calcium sulfate and autologous bone graft . - pubmed
In the second group ( control group ) defect was allowed to heal without PRP , calcium sulfate and autogenous bone graft . - pubmed
Thus the grouping of PRP , combined calcium sulfate with autologous bone graft proved to be a novel osteoinductive biomaterial . - pubmed
TGR was significantly reduced by 12 weeks with lanreotide versus placebo ( difference in least - square mean TGR0 - 12 of - 2.9 [ - 5.1 , - 0.8 ] , p = 0.008 ) , a difference that was maintained at most subsequent visits . - pubmed
baumannii cultures from cerebrospinal fluid ( CSF ) showed extensive resistance to the tested antibiotics except for tigecycline and colistin . - pubmed
While glimepiride stimulates beta - cell secretion and leads to a reduction of blood glucose levels , pioglitazone activates peroxisome proliferator - activated receptor - gamma and improves insulin resistance . - pubmed
The Combined Use of Phenothiazines and Statins Strongly Affects Doxorubicin - Resistance , Apoptosis , and Cox-2 Activity in Colon Cancer Cells . - pubmed
Additionally , COX-2 activity was diminished , and caspase-3 activity was increased to a higher extent by phenothiazine derivative : simvastatin mixtures than by phenothiazine derivatives themselves . - pubmed
We found that SP2509 , a selective inhibitor of LSD1 , specifically promoted the differentiation of Utx - null HSPCs while sparing wild - type HSPCs . - pubmed
In contrast , SP2509 treatment reversed these changes in gene expression in Utx - null HSPCs . - pubmed
Accordingly , Utx loss decreased H3K4 methylation level probably through the COMPASS - like complex , while LSD1 inhibition by SP2509 partially reversed the reduction of H3K4 methylation in Utx - deficient HSPCs . - pubmed
In contrast , DbLcyE preferred to convert lycopene into monocyclic delta - carotene , and possessed a weak beta - monocyclase activity . - pubmed
However , it is rarely reported whether indirect exposure to TCDD can cause similar negative impact on F3 . - pubmed
Results showed that in the F3 generation 500 ng / kg BW / day TCDD group , ovarian coefficient , LH concentration in serum and number of primary follicles were decreased , and the apoptosis of granular cells was significantly increased . - pubmed
Our data showed that ancestral TCDD exposure may impair transgenerational adult ovary development and functions , which may be related to an inhibition of the Igf2 / H19 pathway in the ovarian . - pubmed
The developed approach has been tested using HDAC4 siRNA as a model therapeutics , while the application can also be extended to other gene therapeutics including micro RNA ( miRNA ) , plasmids oligonucleotides , etc . - pubmed
The amino acid sequence of S proein and its interaction with the ACE-2 protein is mimicked with the aid of Hill function . - pubmed
Although the mechanisms of action underlying the glucose - lowering properties of HCQ are still not entirely clear , evidence suggests that this drug may exert multifaceted effects on glucose regulation , including improvement of insulin sensitivity , increase of insulin secretion , reduction of hepatic insulin clearance , and reduction of systemic inflammation . - pubmed
Letter to the editor : " Comment on : Application of the optimized carbon monoxide rebreathing method for the measurement of total haemoglobin mass in chronic liver disease " . - pubmed
This shark displays hormonally controlled bioluminescence in which melatonin ( MT ) and prolactin ( PRL ) trigger light emission , while alpha - melanocyte - stimulating hormone ( alpha - MSH ) and adrenocorticotropic hormone ( ACTH ) play an inhibitory role . - pubmed
Our results reveal the implication of Es - Opn3 , MT , inositol triphosphate ( IP3 ) , intracellular calcium , calcium - dependent calmodulin and dynein in the ILS cell pigment aggregation . - pubmed
The structure , morphology , and valence states of surface elements of as - prepared catalysts were characterized by XRD , TEM , SEM , N2 adsorption - desorption , XPS , and H2 - TPR . - pubmed
BACKGROUND : Lorvotuzumab mertansine ( IMGN901 ) is an antibody - drug conjugate linking an antimitotic agent ( DM1 ) to an anti - CD56 antibody ( lorvotuzumab ) . - pubmed
Only one study showed a positive effect after supplementation with 60,000 IU / month of vitamin D3 for 12 months , with a significant reduction in the concentrations of glycated hemoglobin , fasting glucose , and two - hour postprandial glucose . - pubmed
Further , the molecular interactions between erythrin and the diabetes related target proteins was established by analysing the interactions with associated amino acids . - pubmed
We aimed to evaluate the effect of the inhibitors of proprotein convertase subtilisin / kexin type 9 ( PCSK9 - i ) on steatosis biomarkers such as triglyceride - glucose index ( TyG ) and hepatic steatosis index ( HSI ) and analyse the role of TG / HDL in this population before and after adding - on PCSK9 - i. - pubmed
Basolateral Amygdala Serotonin 2C Receptor Regulates Emotional Disorder - Related Symptoms Induced by Chronic Methamphetamine Administration . - pubmed
Molecular and morphological evidence showed that chronic MA administration reduced the expression of the 5 - hydroxytryptamine ( 5 - HT ) rate - limiting enzyme , tryptophan hydroxylase 2 , in the dorsal raphe and the concentrations of 5 - HT and its metabolite 5 - hydroxyindoleacetic acid in the basolateral amygdala ( BLA ) nuclei . - pubmed
Neuropharmacology and virus - mediated silencing strategies confirmed that targeting 5 - HT2CR reversed the depressive and anxious behaviors induced by chronic MA administration . - pubmed
Thus , herein , for the first time , we report that the abnormal regulation of 5 - HT2CR is involved in the manifestation of emotional disorder - like symptoms induced by chronic MA use . - pubmed
Our study suggests that 5 - HT2CR in the BLA is a promising clinical target for the treatment of MA - induced emotional disorders . - pubmed
Endogenous SO2 - dependent Smad3 redox modification controls vascular remodeling . - pubmed
Notably , ~42 % of these sulfenylated cysteines are dynamically regulated by SO2 , among which is cysteine-64 of Smad3 ( Mothers against decapentaplegic homolog 3 ) , a key transcriptional modulator of transforming growth factor beta signaling . - pubmed
Sulfenylation of Smad3 at cysteine-64 inhibits its DNA binding activity , while mutation of this site attenuates the protective effects of SO2 on angiotensin II - induced vascular remodeling and hypertension . - pubmed
Investigations on new tripeptidyl - p - nitroanilide substrates for subtilisins . - pubmed
Administration of 10(-9)-10(-7 ) mol / L ET caused dose - dependently severe contracture of myocytes , increase in cytosolic lactate dehydrogenase leakage and increase of cellular total calcium . - pubmed
ET also enhanced 45Ca2 + influx in myocytes , that could be antagonized by calcium channel blocker verapamil . - pubmed
Incubation of Fura-2 -preloaded myocytes with ET would induce a significant increase of cytosolic free Ca2 + , and this effect of ET could also be partly antagonized by verapamil . - pubmed
These results suggest that ET enhances Ca2 + influx in myocytes mainly through voltage - dependent calcium channel , by which ET exerts its biological action . - pubmed
The results suggest that Mg2 + ions promote binding by an action at the opioid receptor , even in the absence of G protein , and that opioid antagonists may differ in their recognition of opioid receptor binding sites . - pubmed
[ The effect of elastase on aminonucleoside nephrosis -- on renal vascular system ] . - pubmed
, or PAN+elastase - treated ( 5 mg / kg / day i. m. ) group . - pubmed
These changes , which progressed over a period of 40 min after indomethacin was injected , probably result from renal oligemia and decreased urine production , with an associated decrease in T2 - values . - pubmed
We have explored the effect of the protein kinase inhibitor H7 on tight junction formation in a MDCK cell model for the development of cell - cell contact , tight junctions and epithelial polarity : the " Ca ++ switch " model . - pubmed
In this developmental model , which is thought to mimic processes during the early morphogenesis of epithelial tissues , the protein kinase inhibitor H7 markedly inhibits the development of transepithelial resistance of confluent MDCK cells during the " switch " from low ( 1 - 5 microM ) to normal ( 1.8 mM ) Ca++ media compared with control MDCK cells . - pubmed
Moreover , indirect immunofluorescence using specific antisera against two tight junctional proteins , ZO1 and cingulin , revealed that H7 inhibits the sorting of these proteins from an intracellular site to the lateral surfaces of MDCK cells when the Ca ++ in the medium is raised . - pubmed
Trimming inhibitors , a second generation of inhibitors of glycosylation , interfere with the processing of oligosaccharides by specific glucosidases and mannosidases , resulting in a block of the conversion of high - mannose to complex - type oligosaccharides . - pubmed
Immunoprecipitation using the monoclonal antibodies GR - J2C / 6 and GR - E6B / 3 followed by SDS - PAGE analysis has led to the following results : GR - J2C / 6 precipitated two molecules with apparent molecular weights of 58 and 60 kD ; the antigen recognized by GR - E6B / 3 was a molecule with molecular weight of 68 kD. - pubmed
In includes three successive chromatographies of total tRNA on C18 - modified Lichrosphere Si-1000 in acetonitrile gradients . - pubmed
Clinically , leukopenia with neutropenia and low plasma levels of copper and ceruloplasmin were seen in all cases . - pubmed
ACTH and cortisol production decreases , metabolism slows down and due to an adrenal insufficiency for which the pituitary is responsible there are characteristic changes in the cortisol - dependent metabolic processes . - pubmed
The formation of electronically excited states during hydroperoxide metabolism is analysed in terms of recombination reactions involving secondary peroxyl radicals and scission of the O - O bond of peroxides by haemoproteins , mainly myoglobin . - pubmed
Endogenic phosphorylation of acetyl - CoA - carboxylase completely correlates with its inactivation and depends on the citrate level . - pubmed
About 68 % of individuals receiving PEP had exposures to bites , especially by dogs , and nearly 30 % of PEP cases were due to non - bite exposures . - pubmed
In contrast , with phosvitin as the exogenous substrate , higher doses of polyamines , especially spermine , inhibited phosphorylation . - pubmed
Cytosol protein kinase activity eluted from DEAE - cellulose at 0.2 - 0.3 M NaCl . - pubmed
[ Effect of " heavy " phenamines on alpha - and beta - adrenoreceptors ] . - pubmed
The subunit composition of human myeloperoxidase was studied with the use of sodium dodecyl sulphate / polyacrylamide - gel electrophoresis and gel filtration . - pubmed
The 57 000 - Mr and 39 000 - Mr forms had very similar amino acid composition and yielded an apparently identical collection of fragments on incubation with CNBr . - pubmed
Two hypotheses have been investigated : either a rapid action of glucosidases on the liberated phosphooligosaccharides or a preferential splitting of the non - glucosylated population of dolichyl diphosphate oligosaccharides . - pubmed
Addition of p - nitrophenyl - alpha - D - glucoside inhibits glucosidase activities and allows the production of a major population of dolichyl diphosphate oligosaccharides containing three glucose residues . - pubmed
thiosulphate and cyanide , provides additional evidence of Ping - Pong Bi - Bi mechanism for this transferase . - pubmed
NMR studies of 6 - fluoropyridoxal 5 '- phosphate - containing cytosolic aspartate aminotransferase . - pubmed
Sucrose also led to an early rise in the activity of hepatic triokinase , but did not influence ketohexokinase or fructose-1 - phosphate aldolase . - pubmed
The comparison between the pre- and post - dialysis incorporation values ( 3900 + /- 660 cpm vs 9580 + /- 1600 cpm , n = 8) showed a significant increase of the 3H - Thymidine incorporation in the presence of post - dialysis plasma ( paired t test : p less than 0.01 ) which did not differ from that of control plasma . - pubmed
Serum PSA and testosterone levels were measured monthly . - pubmed
The positive margin rate was not increased after reevaluation with cytokeratin , PSA and prostatic acid phosphatase immuno - staining but of 4 cases initially staged as P0 on hematoxylin and eosin evaluation 2 had microscopic foci of cancer with prostatic acid phosphatase staining . - pubmed
Immuno - staining with the proliferation markers proliferation cell nuclear antigen and Ki-67 showed decreased staining in surgical specimens relative to pretreatment needle biopsy specimens , which suggests that outgrowth of androgen independent clones does not develop during prolonged neoadjuvant therapy . - pubmed
CONCLUSIONS : Eight months of neoadjuvant androgen withdrawal therapy results in low positive margin rates and PSA nadir levels . - pubmed
The initial rapid decrease in PSA results from cessation of androgen regulated PSA synthesis and apoptosis , while the ongoing slower decrease reflects decreasing tumor volume . - pubmed
Polymerization of fibrin monomers in the presence and absence of calcium ions was strongly impaired . - pubmed
SDS - polyacrylamide gel electrophoresis of reduced fibrinogen showed normal B beta - and gamma - chains , whereas no normal A alpha - chain was detected in the proposita . - pubmed
Immunoblot analysis with the monoclonal antibody Y18 , detecting an epitope within the stretch of amino acids A alpha 1 - 51 , revealed an A alpha - chain of about 50 kDa with an intact amino terminus . - pubmed
Immunoblotting with antibodies directed against serum albumin demonstrated the presence of albumin covalently linked to fibrinogen via a disulfide bridge . - pubmed
Radicicol , a protein tyrosine kinase inhibitor , suppresses the expression of mitogen - inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide and in experimental glomerulonephritis . - pubmed
Thus , COX-2 instead of COX-1 is implicated to produce prostanoids mediating inflammatory responses . - pubmed
Radicicol inhibited tyrosine phosphorylation of p53 / 56lyn , a Src family tyrosine kinase and one of the major tyrosine - phosphorylated proteins in LPS - stimulated macrophages . - pubmed
The enzyme activity of COX-1 or COX-2 was not affected by radicicol in macrophages . - pubmed
Other protein tyrosine kinase inhibitors suppressed the LPS - induced COX-2 expression in macrophages but at much higher concentrations than needed for radicicol . - pubmed
Radicicol did not inhibit the COX-2 expression induced by phorbol 12 - myristate 13 - acetate in macrophages . - pubmed
The magnitude of the inhibition of COX-2 protein synthesis by radicicol was much greater than that of the steady state levels of COX-2 mRNA . - pubmed
These results suggest that radicicol inhibits COX-2 expression mainly at post - transcriptional steps . - pubmed
Role of the Cys 2 - Cys 10 disulfide bond for the structure , stability , and folding kinetics of ribonuclease T1 . - pubmed
The Cys 2 - Cys 10 disulfide bond in ribonuclease T1 was broken by substituting Cys 2 and Cys 10 by Ser and Asn , respectively , as present in ribonuclease F1 . - pubmed
The thermodynamic stability of ribonuclease T1 is strongly reduced by breaking the Cys 2 - Cys 10 bond , and the free energy of denaturation is decreased by about 10 kJ / mol . - pubmed
Apparently , the Cys 2 - Cys 10 disulfide bond is important for the stability but not for the folding mechanism of ribonuclease T1 . - pubmed
MDX4 - 4210 and Cosmesil facial elastomers had the strongest bond to skin with the majority of adhesive tapes , whereas Silskin II , Cosmesil HC2 , and RS 330T - RTV were the weakest . - pubmed
Home - made excimer lasers are operating today in the US -- the rest of the country watches and waits . - pubmed
In the MGL line , pyridoxine associated or not with magnesium ( groups 2 and 4 ) significantly reduced the mean number of ulcers . - pubmed
Quaternary structure dynamics and carbon monoxide binding kinetics of hemoglobin valency hybrids . - pubmed
In addition to spermidine , the enzyme also catalyzed the acetylation of spermine and putrescine with Michaelis constants ( Km values ) of 97 , 12 , and 10 microM , respectively . - pubmed
culbertsoni , indicates a role for the enzyme in the production of 1,3 - diaminopropane , the major polyamine found in the Acanthamoeba . - pubmed
Fatty acid composition of plasma and red cell phospholipids was measured within 4 days of birth and at time of ERG testing , and detailed record was kept of dietary intake . - pubmed
A positive correlation was seen between scotopic ( rod ) b - wave implicit time on ERG and DHA in both plasma ( r = 0.733 , P = 0.001 ) and red cells ( r = 0.502 , P = 0.04 ) . - pubmed
A positive correlation was seen between arachidonic acid and rod ERG amplitude ( r = 0.565 , P = 0.022 ) which may reflect the higher AA / DHA ratio in the developing retina . - pubmed
The MESAM IV system measures arterial oxygen saturation ( Sa , O2 ) , heart rate , snoring sounds and body position , and allows both automatic and manual scoring of the recordings . - pubmed
Stereochemistry of oxygenation of linoleic acid catalyzed by prostaglandin - endoperoxide H synthase-2 . - pubmed
The stereochemistry of the hydrogen which was removed from C-11 during the conversion of linoleic acid into hydroxy acids in the presence of PGHS-1 or PGHS-2 was determined by incubation of [ ( 11R )- 2H ]- and [ ( 11S )- 2H ] linoleic acids followed by mass spectrometric analysis of the isotope contents of the individual hydroxy acid isomers . - pubmed
Both enzymes were found to catalyze oxygenations which involved stereospecific removal of the ( 11S ) hydrogen and retention of the ( 11R ) hydrogen . - pubmed
It is concluded that the initial steps of the PGHS-2- and PGHS-1 - catalyzed oxygenations proceed with identical stereochemistry and involve stereospecific removal of the pro - S hydrogen from the omega 8 - methylene group of the substrate . - pubmed
We were not able to characterize a Ca2 + stimulated ATPase , whereas 57 and 15 % of the total ATPase activity were sensitive to Mg2 + in the oviductal and endometrial fractions , respectively . - pubmed
Increasing the preincubation time to 30 min , the Mg2 + ATPase in the endometrial fraction was significantly inhibited by all four pesticides at 64 microM , but in the oviductal fraction only at 64 microM o , p ''- DDT . - pubmed
The insulin suppression test consisted of a constant infusion of glucose , insulin and somatostatin for 150 min ; insulin resistance was estimated by determining the steady - state plasma glucose concentrations during the last 60 minutes of the test . - pubmed
These results have also provided in vivo evidence that DAG - PKC activation could be responsible for the hyperglycemia - induced vascular dysfunctions in diabetes . - pubmed
Based on the demand for cholesterol for membrane formation , we determined the ability of low - density lipoprotein ( LDL ) to support proliferation in lymphocytes bearing different LDL receptor mutations , which were treated " in vitro " with lovastatin to inhibit endogenous cholesterol synthesis . - pubmed
Flow cytometric analysis with 1,1 '- dioctadecyl-3,3,3,3 '- tetramethylindocarbocyanineperchlorate ( Dil)-LDL showed normal LDL binding but defective internalization in lymphocytes from case 1 , whereas in lymphocytes from case 2 both LDL binding and internalization were affected . - pubmed
No methylated or demethylated products of DMA were detected in blood or any organ up to 8 h post - exposure . - pubmed
In this report , we demonstrate that Us9 - promoted transneuronal spread in vivo is dependent on a conserved acidic motif previously shown to be essential for the maintenance of Us9 in the TGN region and recycling from the plasma membrane . - pubmed
Mutant viruses with the acidic motif deleted have an anterograde spread defect indistinguishable from that of Us9 null viruses . - pubmed
The compounds have been tested for their inhibitory effects on Epstein - Barr virus early antigen ( EBV - EA ) activation induced by 12 - O - tetradecanoylphorbol-13 - acetate ( TPA ) , as a test for potential cancer chemopreventive agents . - pubmed
However , altering two amino acid residues in the EII ' enzyme enhanced the activity of EII ' to the same level as that of the EII enzyme . - pubmed
The deduced amino acid sequences from eight regions of pOAD2 had significant similarity with the sequences of gene products such as oppA - F ( encoding oligopeptide permease ) , ftsX ( filamentation temperature sensitivity ) , penDE ( isopenicillin N - acyltransferase ) and rep ( plasmid replication ) . - pubmed
The data indicate that ( a ) both c - Fos and p53 are involved in cellular protection against the clastogenic effect of genotoxic agents , ( b ) p53 is not required for induction of apoptosis by UV light and MMS , but rather prevents fibroblasts from undergoing apoptotic cell death upon DNA damage , and ( c ) c - Fos and p53 seem to act independently in determining genotoxic resistance , which is hypothesized to be achieved by impaired DNA repair or differential cell cycle check point control . - pubmed
Furthermore , mechanisms such as direct inhibition of oxygen free radical formation , abrogation of neutrophilic infiltration and reduced up - regulation of adhesion molecules have also been documented in these studies . - pubmed
The cytochrome P4503A4 inhibitor clarithromycin increases the plasma concentrations and effects of repaglinide . - pubmed
Plasma repaglinide , serum insulin , and blood glucose concentrations were measured up to 7 hours . - pubmed
Clarithromycin increased the mean incremental area under the concentration - time curve from 0 to 3 hours of serum insulin by 51 % ( P & lt;.05 ) and the maximum increase in the serum insulin concentration by 61 % ( P & lt;.01 ) compared with placebo . - pubmed
CONCLUSIONS : Even low doses of the cytochrome P4503A4 ( CYP3A4 ) inhibitor clarithromycin increase the plasma concentrations and effects of repaglinide . - pubmed
Concomitant use of clarithromycin or other potent inhibitors of CYP3A4 with repaglinide may enhance its blood glucose - lowering effect and increase the risk of hypoglycemia . - pubmed
Administration of an AA metabolism inhibitor , however , only blocked the stimulatory effect of sGnRH without affecting cGnRH - II - induced meiosis . - pubmed
As observed previously , in the presence of GtH , sGnRH was found to inhibit GtH - induced resumption of meiosis and testosterone production , whereas cGnRH - II was without effect . - pubmed
Platelet - derived growth factor activates production of reactive oxygen species by NAD ( P ) H oxidase in smooth muscle cells through Gi1,2 . - pubmed
Recent findings indicate that platelet - derived growth factor ( PDGF ) plays a role in the generation of reactive oxygen species ( ROS ) as second messengers in smooth muscle cells ( SMC ) . - pubmed
ROS release was completely inhibited by the Gi protein inhibitor PTX as well as an antibody against Galphai1,2 , however , not by antibodies against Galphai3 / 0 , Gas , and Gbeta1beta2 . - pubmed
Immunoaffinity chromatography demonstrated coupling of Galphai1,2 to the PDGF a - receptor , which , after preincubation of the SMC membranes with PDGF , was increased in the absence of GTPgammaS but decreased in the presence of GTPgammaS and prevented by PTX treatment . - pubmed
Analyses of both discrete traits ( type 2 diabetes and the composite trait of type 2 diabetes and/or impaired glucose homeostasis [ IGH ] ) , and quantitative traits ( glucose levels during a 75 - g oral glucose challenge , designated glucose 0 - 180 and HbA(1c ) ) were performed . - pubmed
These results suggest that the antibodies prepared against omega - CTX GVIA and B1Nt in this work have high selectivity for their antigen . - pubmed
Gastric intramucosal pH ( pHi ) , plasma endotoxin in portal vein , and plasma D - lactic acid were measured and blood samples were cultured at different time intervals after trauma . - pubmed
Using urea denaturation , coat protein monomers are shown to be marginally stable . - pubmed
The reversible folding of coat protein follows a three - state model , N if I if U , with an intermediate exhibiting most of the tryptophan fluorescence of the folded state , but little secondary structure . - pubmed
The specific binding of L - tryptophan to serum albumin . - pubmed
Purification of native TSH receptor has allowed to study directly and to characterize the autoantibodies directed against this antigen . - pubmed
The use of triisopropylsilyl - oxymethyl ( TOM ) in the synthesis of anti - telomerase 2 - 5A antisense compound RBI 011 . - pubmed
The 2 - 5A antisense compound RBI 011 targeting telomerase RNA was synthesized using the triisopropylsilyl - oxymethyl ( TOM ) group for the 3 '- hydroxyl protection of 2',5'-linked RNA . - pubmed
In the final steps of glycogenolysis , intracellular glucose 6 - phosphate ( Glc-6 - P ) is transported into the endoplasmic reticulum where it is hydrolyzed to glucose by glucose-6 - phosphatase ( Glc-6 - Pase ) . - pubmed
Although the majority of body glycogen is stored in the muscle , the current dogma holds that Glc-6 - Pase ( now named Glc-6 - Pase - alpha ) is expressed only in the liver , kidney , and intestine , implying that muscle glycogen can not contribute to interprandial blood glucose homeostasis . - pubmed
Ten years ago , the linkage between mutations in the gene coding for the antioxidant enzyme Cu , Zn superoxide dismutase ( SOD1 ) and the neurodegenerative disease known as familial amyotrophic lateral sclerosis ( FALS ) was established . - pubmed
Increased glutathione ( gamma - glutamylcysteinylglycine ; GSH ) conjugation ( inactivation ) of alkylating anticancer drugs due to overexpression of cytosolic glutathione S - transferase ( GST ) is believed to be an important mechanism in tumor cell resistance to alkylating agents . - pubmed
In our experiments , a combination of lipid chromatography / electrospray ionization mass spectrometry ( LC / ESI / MS ) was employed for structural characterization of the resulting conjugates between CHB and GSH . - pubmed
We showed that microsomal GST was activated by CHB in a concentration and time dependent manner . - pubmed
Microsomal GST which was stimulated approximately two - fold with CHB had a stronger catalytic effect . - pubmed
Thus , microsomal GST may play a potential role in the metabolism of CHB in biological membranes , and in the development of ADR . - pubmed
Our results show that survival factor NT-3 acts as a short - distance axon guidance molecule for muscle sensory afferents as they approach their proper targets . - pubmed
SNP - stimulated ( 18 ) F - FDG uptake was abolished by cotreatment with cycloheximide , the tyrosine kinase inhibitor genistein , the phosphatidylinositol-3 kinase ( PI3 K ) inhibitor wortmannin , or the protein kinase C inhibitor staurosporine . - pubmed
CONCLUSION : NO stimulates ( 18 ) F - FDG uptake in HUVECs through an increase in GLUT1 expression and hexokinase activity , which appears to involve both protein kinase C and PI3 K pathways . - pubmed
Using semiquantitative reverse transcription - PCR , the genes of the gal operon were shown to be expressed more during growth in iron medium than in growth in sulfur medium . - pubmed
RESULTS : While many old drugs are truly antiquated , medications such as typical antipsychotics , tricyclic and monoamine oxidase antidepressants , and lithium should remain integral parts of a psychopharmacology teaching program . - pubmed
Insulin sensitivity derived from oral glucose tolerance testing in athletes : disagreement between available indices . - pubmed
The aims of the present study were to determine whether available " fasting " and oral glucose tolerance test - derived insulin sensitivity indices could effectively discriminate between individuals with higher than normal insulin sensitivity , and whether they would all provide similar information in clinical practice . - pubmed
Besides glucose and insulin responses , a total of nine such indices were computed . - pubmed
Fasting as well as post - load glucose concentrations were similar in the three groups , while basal plasma insulin and the insulinaemic response to glucose were both higher in untrained individuals ( at P & lt ; 0.05 and P & lt ; 0.02 , respectively ) . - pubmed
Apparently , the various insulin sensitivity indices examined provided different quantitative and qualitative information , despite insulin action being greater in both groups of athletes relative to controls , as reflected by their similar glucose tolerance with lower insulin concentrations . - pubmed
Cu , Zn - superoxide dismutase is an intracellular catalyst for the H ( 2 ) O ( 2 )-dependent oxidation of dichlorodihydrofluorescein . - pubmed
We compared the peroxidase activity of Cu , Zn - superoxide dismutase ( CuZnSOD ) , cytochrome c , horseradish peroxidase ( HRP ) , Cu ( 2 + ) , and Fe ( 3 + ) under various condi - tions to identify an intracellular catalyst . - pubmed
Enormous increase by bicarbonate in the rate of DCFH ( 2 ) oxidation distinguished CuZnSOD from cytochrome c and HRP . - pubmed
Cyanide inhibited the reaction catalyzed by CuZnSOD but accelerated that by Cu ( 2 + ) and Fe ( 3 + ) . - pubmed
We propose that CuZnSOD is a potential intracellular catalyst for the H ( 2 ) O ( 2 )-dependent oxidation of DCFH ( 2 ) . - pubmed
The addition of cross - linked murein , disaccharides linked to interconnected wall peptides , blocked GFP - CWT binding to staphylococci , whereas murein monomers or lysostaphin - solubilized cell wall fragments did not . - pubmed
A tagO mutant strain , defective in the synthesis of polyribitol wall teichoic acid attached to the cell wall envelope , displayed increased GFP - CWT binding . - pubmed
In contrast , a femAB mutation , reducing both the amount and the length of peptidoglycan cross - linking ( monoglycine cross bridges ) , showed a dramatic reduction in GFP - CWT binding . - pubmed
Pretreatment with phenoxybenzamine , an alpha adrenoceptor blocking agent , resulted in a small but statistically significant attenuation in the response of SCBF to brushing . - pubmed
However , pretreatment with propranolol , a beta adrenoceptor blocking agent , or atropine , a muscarinic cholinergic receptor blocking agent , produced no such effect . - pubmed
In CT investigation irregular bone thickenings of anterior and lateral walls of maxillary sinus were visible . - pubmed
This process is notably exemplified by the discovery of the modified betulinic acid derivative , DSB [ PA-457 ] , which is currently in Phase II clinical trial and is the first - in - class HIV maturation inhibitor ( MI ) . - pubmed
In contrast , addition of females injected with Ovaprim ( to induce an LH surge and ovulation ) increased male LH concentrations at all sample times ( 5 , 9 , 13 , and 17h ) following female addition and increased milt volumes at all but the first ( 5h ) sample time . - pubmed
Males also increased LH and milt 9h after addition of females injected with the goldfish pheromonal steroid 4 - pregnen-17,20beta - diol-3 - one ( 17,20betaP ) , suggesting that similar responses to ovaprim - injected females were due , at least in part , to release of preovulatory pheromonal steroid(s ) . - pubmed
Recent work has identified a role for metabotropic glutamate receptor subtype 5 ( mGlu ( 5 ) ) in the discriminative stimulus properties of investigator - administered ethanol . - pubmed
The purpose of this study was to determine if mGlu ( 5 ) receptors modulate the discriminative stimulus properties of self - administered ethanol . - pubmed
Results show that the mGlu ( 5 ) receptor antagonist 6 - Methyl-2 -( phenylethynyl ) pyridine ( MPEP ; 10 mg / kg ) inhibited the discriminative stimulus properties of consumed ethanol during a self - administration test session . - pubmed
These results indicate that mGlu ( 5 ) receptors are involved in the expression of the discriminative stimulus properties of self - administered ethanol . - pubmed
OBJECTIVES : Methionine synthase reductase ( MTRR ) and betaine - homocysteine S - methyltransferase ( BHMT ) are two enzymes that regulate homocysteine metabolism . - pubmed
We further evaluated whether homocysteine levels differed between cases and controls for MTRR and BHMT genotypes . - pubmed
The etx gene on these plasmids was insertionally inactivated using a chloramphenicol resistance cassette to phenotypically tag the plasmid . - pubmed
The synthesized 1,8 - naphthyridine-3 - carboxamides were also evaluated for anti - inflammatory and myeloprotective activities , indicated by modulation in cytokine and chemokine levels secreted by dendritic cells . - pubmed
After deletion of the central anaerobic fermentation genes ( ldhA , adhE , ackA ) , the pathway for malate and succinate production remained as the primary route for the regeneration of NAD + . - pubmed
Under anaerobic conditions , ATP production for growth was obligately coupled to malate dehydrogenase and fumarate reductase by the requirement for NADH oxidation . - pubmed
The best succinate biocatalysts , strains KJ060(ldhA , adhE , ackA , focA , pflB ) and KJ073(ldhA , adhE , ackA , focA , pflB , mgsA , poxB ) , produce 622 - 733 mM of succinate with molar yields of 1.2 - 1.6 per mole of metabolized glucose . - pubmed
The best malate biocatalyst , strain KJ071(ldhA , adhE , ackA , focA , pflB , mgsA ) , produced 516 mM malate with molar yields of 1.4 per mole of glucose metabolized . - pubmed
Angiotensin - converting enzyme inhibitors , angiotensin II receptor blockers , and statins have renoprotective effects . - pubmed
Only captopril consistently improved these abnormalities and reduced the cortical expression of several proinflammatory and profibrotic factors including transforming growth factor - beta ( TGF - beta ) . - pubmed
These effects were independent of blood pressure , accompanied by increased urine N - acetylseryl - aspartyl - lysyl - proline ( Ac - SDKP ) levels , and the restoration of renal angiotensin - converting enzyme and angiotensin II to baseline levels . - pubmed
Captopril or Ac - SDKP suppressed angiotensin II - induced MAPK activation and TGF - beta secretion . - pubmed
Angiotensin - converting enzyme inhibition confers renoprotection in adriamycin nephropathy by reducing intrarenal angiotensin II and augmenting Ac - SDKP expression that together attenuate MAPK signaling and its downstream proinflammatory and fibrogenic properties . - pubmed
The degree of alkylation ranged from methyl ( C1 ) to heptyl ( C7 ) and represents the types of APs present in produced water . - pubmed
Independent of alkyl homologue , the glucuronide conjugated APs were the most abundant metabolites ( 73 - 100 % ) , whereas sulfates , glucosides and unchanged compounds were excreted in amounts of 0 - 21 % , 0 - 6.1 % and 0 - 6.3 % , respectively . - pubmed
PURPOSE : Romidepsin ( FK228 , depsipeptide , FR901228 ) , a unique cyclic depsipeptide with a histone deacetylase inhibitor ( HDACI ) activity , is a potential cancer therapeutic agent and currently under clinical trials for several types of cancer . - pubmed
Our data indicate that DMCA can potentiate the HDACI activity of romidepsin and other biological activities , such as cell cycle arrest and apoptosis ; these effects were accompanied with the expression of various key cell cycle regulators in different bladder cancer cells . - pubmed
FT - IR , FT - Raman spectra and ab initio HF and DFT calculations of 4 - N , N '- dimethylamino pyridine . - pubmed
Thyroid tests at birth , age at TSH normalization and average thyroid tests under L - T4 treatment during the 1st yr did not differ between sexes in any of the groups . - pubmed
In both cell lines , MI - ER1 alpha interacted with ER alpha in the presence and absence of oestrogen , but the interaction was stronger in the absence of ligand . - pubmed
17 - AAG sensitized malignant glioma cells to death - receptor mediated apoptosis . - pubmed
17 - AAG is a selective HSP90 - inhibitor that exhibited therapeutic activity in cancer . - pubmed
Treatment with subtoxic doses of 17 - AAG in combination with tumor necrosis factor - related apoptosis - inducing ligand ( TRAIL ) induces rapid apoptosis in TRAIL - resistant glioma cells , suggesting that this combined treatment may offer an attractive strategy for treating gliomas . - pubmed
17 - AAG treatment down - regulated survivin through proteasomal degradation . - pubmed
In addition , over - expression of survivin attenuated cytotoxicity induced by the combination of 17 - AAG and TRAIL . - pubmed
Cytochrome P450 monooxygenase appears to be involve in the first step of 1,2,4 - TCB degradation , as evidenced by marked inhibition of both dechlorination and degradation of 1,2,4 - TCB in the presence of the known cyt P450 inhibitors 1 - aminobenzotriazole and piperonyl butoxide . - pubmed
Based on these results , a degradation pathway of 1,2,4 - TCB through cyt P450 monooxygenase and epoxide hydrolase was proposed . - pubmed
The neutron transmutation doping ( NTD ) method was applied to the initially p - type silicon in order to extend the NTD applications at HANARO . - pubmed
Our goal was to investigate the effects of exposure to nicotine on maternal , fetal , and neonatal IGF - II processing . - pubmed
Nicotine exposure prevented the decrease in maternal IGF - II processing seen in controls with advancing gestation . - pubmed
However , there was no influence of nicotine on fetal levels of IGF - II . - pubmed
Postnatally ( postnatal day [ PND ] 21 ) , pups exposed to nicotine in utero had decreased levels of big IGF - II . - pubmed
Our results show , for the first time , that nicotine exposure prevents the decrease of IGF - II processing in the maternal compartment . - pubmed
Sequencing of the complete genome of one of these variants identified a single point mutation that resulted in a substitution of a conserved asparagine by histidine at position 498 of the haemagglutinin - neuraminidase protein , although this mutation was not present in all rapidly growing variants . - pubmed
3 - Bromoacetamino-4 - methoxy - benzoylurea ( F13 ) is a benzoylurea derivative selected from the library of small molecule tubulin ligands . - pubmed
This was found to occur through the upregulation of the reversion - inducing cysteine - rich protein with Kazal motifs ( RECK ) , a membrane - anchored inhibitor of MMPs , which acts by reducing ERK1 / 2 phosphorylation . - pubmed
Data collection will take place during the Physical Education ( PE ) lessons and on school premises and will include : anthropometrical variables ( height , weight , waist and hip circumferences , skinfold thickness at two sites ) , physiological responses ( blood pressure , aerobic fitness , heart rate ( HR ) response , vertical jump performance , 10 - metre ( m ) sprint , 50 - m sprint and 505 - agility test ) , diet ( self - reported seven - day food diary ) , physical activity ( Physical Activity Questionnaire for Adolescents ( PAQ - A ) ) and blood tests ( fasting glucose , insulin , total cholesterol ( TC ) , high - density lipoprotein ( HDL ) , high - sensitivity C - reactive protein ( hs - CRP ) , fibrinogen ( Fg ) , interleukin-6 ( IL-6 ) , adiponectin ( high molecular weight ) , triglyceride and plasminogen activator inhibitor-1 ( PAI-1 ) . - pubmed
The diagnosis of MNGIE disease can be established in a proband by detection of one of the following : ( 1 ) biallelic pathogenic variants in TYMP ( formerly known as ECGF1 ) ; ( 2 ) markedly reduced levels of thymidine phosphorylase enzyme activity ; or ( 3 ) elevated plasma concentrations of thymidine and deoxyuridine . - pubmed
PR - Set7 - mediated monomethylation of histone H4 lysine 20 at specific genomic regions induces transcriptional repression . - pubmed
Lipoprotein lipase ( LPL)-mediated lipolysis of triglycerides is the first and rate - limiting step in chylomicron / very low density lipoprotein clearance at the luminal surface of the capillaries . - pubmed
Moreover , ANGPTL3 enhances LPL cleavage in the presence of either heparan sulfate proteoglycans or glycosylphosphatidylinositol - anchored high density lipoprotein - binding protein 1 ( GPIHBP1 ) . - pubmed
In this study , using a placental explant model , we investigated whether different oxygen environments induced abnormal shedding of trophoblastic debris or secretion of cytokines from the placenta . - pubmed
There was also no difference in the levels of activated caspases in trophoblasts shed from explants cultured in these two oxygen environments nor was there a significant difference in the endothelial cell responses to trophoblasts shed from explants cultured in 1 % or 8 % oxygen . - pubmed
Similarly , there was no significant change in the secretion of nine cytokines into the conditioned medium from explants cultured in 1 % or 8 % oxygen . - pubmed
RESULTS : The mixed venous oxygen saturation ( SvO(2 ) ) was lower through T2 - T4 compared to the baseline value in both groups , while the degree of the decrease was significantly less in group M than that in group C. The other hemodynamic variables , the operative data and the postoperative outcomes were similar between the two groups . - pubmed
Matrix - assisted laser desorption / ionization ( MALDI ) was performed on an external ion source Fourier transform ion cyclotron resonance mass spectrometer ( FTICR - MS ) to analyze the block length distributions of triblock polymers of poly ( oxypropylene ) and poly ( oxyethylene ) . - pubmed
CBD elicits an endoplasmic reticulum ( ER ) stress response , characterized by changes in ER morphology and the initiation of RNA - dependent protein kinase - like ER kinase- , activating transcription factor-6- , and inositol - requiring ER - to - nucleus signal kinase-1 ( IRE1)-mediated signaling cascades . - pubmed
Furthermore , CBD induces downstream activation of the pro - apoptotic IRE1 / ASK1 / c - Jun N - terminal kinase pathway , leading to HSC death . - pubmed
Finally , we provide evidence that the elevated basal level of ER stress in activated HSCs has a role in their susceptibility to the pro - apoptotic effect of CBD . - pubmed
DeltaywdL spores are unable to germinate in response to CaDPA , and have altered germination properties , a phenotype that confirms the expected defect in localization of the cortex lytic enzyme CwlJ in the coat . - pubmed
The repair of cis - syn cyclobutane pyrimidine dimers ( CPDs ) can be initiated via the base excision repair ( BER ) pathway , utilizing pyrimidine dimer - specific DNA glycosylase / lyase enzymes ( pdgs ) . - pubmed
High purity recombinant proteins rEhCBP30 , rEhCBP30 - CHP and rEhCBP30 - ATP with N - terminal His tag were obtained by single step affinity purification . - pubmed
After a gap of almost 60 years following the development of warfarin , 2 new categories of oral anticoagulant agents have been approved for clinical use - the direct thrombin inhibitors and factor Xa inhibitors . - pubmed
There is a difference between immature and adult brain in an appearance of withdrawal symptom after administration of the partial agonist of benzodiazepine receptors Ro 19 - 8022 . - pubmed
Lysophosphatidic acid promotes cell migration through STIM1- and Orai1 - mediated Ca2 +( i ) mobilization and NFAT2 activation . - pubmed
Here we investigated the involvement of agonist - induced Ca ( 2 + ) entry and STIM1 and Orai1 proteins in regulating nuclear factor of activated T cell ( NFAT ) signaling and LPA - induced keratinocyte cell motility . - pubmed
Store - operated Ca ( 2 + ) entry ( SOCE ) was blocked by the SOCE inhibitor diethylstilbestrol ( DES ) , STIM1 silencing using RNA interference ( RNAi ) , and expression of dominant / negative Orai1(R91W ) . - pubmed
LPA induced significant NFAT activation as monitored by nuclear translocation of green fluorescent protein - tagged NFAT2 and a luciferase reporter assay , which was impaired by DES , expression of Orai1(R91W ) , and inhibition of calcineurin using cyclosporin A ( CsA ) . - pubmed
By using chemotactic migration assays , LPA - induced cell motility was significantly impaired by STIM1 , CsA , and NFAT2 knockdown using RNAi . - pubmed
These data indicate that in conditions relevant to epidermal wound healing , LPA induces SOCE and NFAT activation through Orai1 channels and promotes cell migration through a calcineurin / NFAT2 - dependent pathway . - pubmed
The effect of tamoxifen was considered a clinical complete response ( CR ) . - pubmed
Telmisartan administration resulted in significant suppression in tumor necrosis factor - alpha expression and nuclear factor - kappaB phosphorylation . - pubmed
The two - component system ( TCS ) KdpD / KdpE , extensively studied for its regulatory role in potassium ( K(+ ) ) transport , has more recently been identified as an adaptive regulator involved in the virulence and intracellular survival of pathogenic bacteria , including Staphylococcus aureus , entero - haemorrhagic Escherichia coli , Salmonella typhimurium , Yersinia pestis , Francisella species , Photorhabdus asymbiotica , and mycobacteria . - pubmed
Here we employ STM to study individual tris -( phenylpyridine )- iridium complexes on a C60 monolayer , and investigate the influence of their electronic structure on the plasmon excitation between the Ag ( 111 ) substrate and an Ag - covered Au tip . - pubmed
Prospective and post - hoc analyses from these phase III studies show rasagiline monotherapy significantly improved tremor symptoms in early PD , independent of disease duration , compared with placebo . - pubmed
A six - week treatment of weekly intraperitoneal injections of PNP ( 50 mg / kg ) resulted in severe damage to the seminiferous tubules , a remarkable increase in both hydroxyl radical and malondiadehyde production , and notably decreased glutathione peroxidase and superoxide dismutase activities . - pubmed
Moreover , PNP treatment induced germ cell apoptosis , inhibited Bcl - xl expression , and then activated Bax expression and the caspase-3 enzyme . - pubmed
However , simultaneous supplementation with quercetin ( 75 mg / kg ) attenuated the toxicity induced by PNP through renewal of the antioxidant enzyme 's status , alleviating apoptosis by regulating the expressions of Bax and Bcl - xl , XBP-1 and HO-1mRNAs , and the regulation of caspase-3 activity . - pubmed
This study compared five technology configurations : 1 - conventional AD with CHP , 2 - Thermal Hydrolysis Process ( THP ) AD with CHP , 3 - THP AD with bio - methane grid injection , 4 - THP AD with CHP followed by drying of digested sludge for solid fuel production , 5 - THP AD followed by drying , pyrolysis of the digested sludge and use of the both the biogas and the pyrolysis gas in a CHP . - pubmed
Towards achieving this goal , several researchers have carried out studies in various cell lines and in vivo and have deciphered that fucoxanthin exerts its anti - proliferative and cancer preventing influence via different molecules and pathways including the Bcl-2 proteins , MAPK , NFkappaB , Caspases , GADD45 , and several other molecules that are involved in either cell cycle arrest , apoptosis , or metastasis . - pubmed
The amino acid sequence of the protein corresponded to that of albumin . - pubmed
Toxicity and quality of life after choline - PET / CT directed salvage lymph node dissection and adjuvant radiotherapy in nodal recurrent prostate cancer . - pubmed
BACKGROUND : In a previous study we demonstrated that , based on 11C / 18 F - choline positron emission tomography - computerized - tomography as a diagnostic tool , salvage lymph node dissection ( LND ) plus adjuvant radiotherapy ( ART ) is feasible for treatment of pelvic / retroperitoneal nodal recurrence of prostate cancer ( PCa ) . - pubmed
In general , TRPs are nonselective cation channels which are Ca ( 2 + ) permeable . - pubmed
Fibroin 1 is dominated by glycine - alanine motifs , and lacks sequence motifs associated with orb - weaver MaSps . - pubmed
We hypothesize fibroin 2 is a piriform or aciniform silk protein , based on amino acid composition , spigot morphology , and phylogenetic analyses . - pubmed
To overcome this immediate lethality , we generated a doxycycline - responsive tTA - DNMT1 * rescue line and readily obtained homozygous DNMT1 - mutant lines . - pubmed
However , doxycycline - mediated repression of exogenous DNMT1 * initiates rapid , global loss of DNA methylation , followed by extensive cell death . - pubmed
Characterization of JNJ-42847922 , a Selective Orexin-2 Receptor Antagonist , as a Clinical Candidate for the Treatment of Insomnia . - pubmed
Here , we characterized JNJ-42847922 ( [ 5 -( 4,6 - dimethyl - pyrimidin-2 - yl )- hexahydro - pyrrolo [ 3,4 - c ] pyrrol-2 - yl ]-( 2 - fluoro-6 -[ 1,2,3 ] triazol-2 - yl - phenyl )- methanone ) , a high - affinity / potent OX2R antagonist . - pubmed
JNJ-42847922 had an approximate 2 - log selectivity ratio versus the human orexin-1 receptor . - pubmed
Associations between the dysregulation of the hypothalamic - pituitary - adrenal ( HPA ) axis and these disorders have been recognized in the scientific literature , specifically in regard to cortisol levels , as well as changes in pro- and anti - inflammatory cytokines . - pubmed
Identification of a resveratrol tetramer as a potent inhibitor of hepatitis C virus helicase . - pubmed
Combined treatment of vitisin B with an NS5B polymerase inhibitor ( sofosbuvir ) exhibited a synergistic or at least additive antiviral activity . - pubmed
We confirmed a direct binding between vitisin B and a purified NS3 helicase in vitro . - pubmed
Vitisin B was a potent inhibitor of a HCV NS3 helicase ( IC50 = 3 nM ) . - pubmed
Conclusion and Implications Vitisin B is one of the most potent HCV helicase inhibitors identified so far . - pubmed
scoparia and to determine the relationship of EPSPS gene amplification with the level of glyphosate resistance . - pubmed
In the absence of glyphosate , no differences in growth and reproductive parameters were evident between the tested GR and SUS inbred lines , across an intraspecific competition gradient ( 1 to 170 plants m-2 ) . - pubmed
scoparia plants with 2 to 4 copies of the EPSPS gene survived the field - use rate ( 870 g ha-1 ) of glyphosate , but failed to survive the 4,350 g ha-1 rate of glyphosate ( five - times the field - use rate ) . - pubmed
In contrast , GR plants with 5 to 14 EPSPS gene copies survived the 4,350 g ha-1 of glyphosate . - pubmed
scoparia with 5 or more EPSPS gene copies will most likely persist in field populations , irrespective of glyphosate selection pressure . - pubmed
Analysis of variance ( ANOVA ) and Tukey 's test were used to verify any differences in creatinine levels and estimated clearance at 0 , 6 , 12 , 24 and 36 months , adjusting for the co - variables sex , skin color , age & gt;50 years , arterial hypertension , diabetes and the use of the ritonavir - boosted protease inhibitors ( PI / r ) lopinavir / r or atazanavir / r. The software package STATISTICA 10 ( ) was used for statistical analysis . - pubmed
Approximately 27 deaths could be avoided for every 1000 people receiving NLT of beta - adrenergic blocking agents , ACEIs , and ARBs . - pubmed
Participants in the NLT group reached maximal dose of beta - adrenergic blocking agents in half the time compared with participants in usual care . - pubmed
In one scenario , the loss of function of lipoic acid synthase , an enzyme with Fe - S cluster cofactor in mitochondria , impairs activity of various lipoamide - dependent enzymes with drastic consequences for metabolism . - pubmed
In a third scenario , tRNA modifications arising from action of the cysteine desulfurase and/or Fe - S cluster proteins are lost , which may lead to impaired protein synthesis . - pubmed
METHODS : MATRICS Consensus Cognitive Battery ( MCCB ) performance was assessed in CPD ( n = 41 ) and healthy subjects ( HS ; n = 41 ) in a double - blind , randomized cross - over design of acute ( single dose ) MEM ( placebo vs. 10 or 20 mg p.o . ) - pubmed
In CPD subjects , greater age , positive MEM effects on PPI , and SNP rs1337697 ( within the ionotropic NMDA receptor gene , GRIN3A ) predicted greater positive OCME with 20 mg MEM . - pubmed
First , elevated GTSP1 expression in SD patients might be the result of activation of GTSP1 in response to exposure to carcinogens . - pubmed
Composed of ten subunits that add up to a molecular mass of about 500 kDa , TFIIH is also essential for nucleotide excision repair . - pubmed
Geological evidence suggests that after the Late Pleistocene closure of the Danakil - Red Sea connection , increased post - glacial groundwater availability caused the formation of a brackish paleo - lake flooding the entire region below the -50 m contour . - pubmed
MTX concentrations were not significantly different in the brain or CSF 6 hr after the intrathecal administration of MTX . - pubmed
However , compare to intravenous administration of MTX , intravenous administration of both FA and MTX significantly decreased MTX concentrations in the brains and CSF . - pubmed
These results suggest that FA inhibits the influx of MTX into the brain and CSF , possibly by competing with folate carriers , but has no effect on its efflux from these regions . - pubmed
This paper presented the studies on effects of various concentrations of BPA ( 0 , 0.25 mg kg-1 , 0.50 mg kg-1 , 1.00 mg kg-1 , 2.00 mg kg-1 ) and soil aertaiton ( aerobic and anaerobic ) on characteristics of paddy soil microbial communites by technology of qPCR ( fluorescence quantitative PCR ) and PCR - DGGE ( polymerase chain reaction - denaturing gradient gel electrophoresis ) . - pubmed
Two - month treatment with HCQ resulted in significant decrease in SLEDAI-2 K ( p & lt ; 0.001 ) , anti - dsDNA ( p & lt ; 0.001 ) , IL-1beta ( p = 0.003 ) , IL-6 ( p & lt ; 0.001 ) and TNF - alpha ( p & lt ; 0.001 ) and a significant increase in CH50 levels ( p = 0.012 ) . - pubmed
Compounds 2 - 6 showed potent in vitro pancreatic lipase inhibitory activity , suggesting their participation in the reductive effect of the herbs on triglyceride absorption . - pubmed
Pretreatment with baicalin decreased the inflammatory response ; pretreatment with baicalin and berberine hydrochloride decreased the inflammatory response mediated by the NF - kappaB signaling pathway . - pubmed
Eco - friendly hydrogel composite beads based on crosslinked - carboxymethyl cellulose ( CMC ) and dextran sulfate ( DS ) embedded within network were prepared using ionotropic gelation in presence of sodium n - dodecyl sulfate ( SDS ) as pore - forming template . - pubmed
The effect of DS on adsorption of composites for cationic dye methylene blue ( MB ) was investigated by changing influencing factors : pH , adsorbent dosage , time contact , dye concentration , and temperature . - pubmed
Further LC - MS analysis confirmed the similarity of fungal resveratrol with standard resveratrol . - pubmed
CLP - protein binding assay following proteome analysis reveals that iturin A attached to 12 - oxophytodienoate reductase 2 involved in the oxylipin biosynthetic process required for jasmonic acid production which is implicated in induced systemic resistance ( ISR ) . - pubmed
This study suggests specific elicitor action of individual CLP , particularly iturin A showed the most powerful in stimulating the ISR system in response to stresses in postharvest mandarins . - pubmed
Both ligands have been previously developed as mimics of the tetrapeptide sequence Asp1 - Trp2 - Val3 - Ile4 of the cadherin adhesion arm , and have been shown to inhibit E - cadherin - mediated adhesion in epithelial ovarian cancer cells with millimolar potency . - pubmed
Overall , these results will help the design of novel cadherin inhibitors that might prevent the swap dimer formation by targeting both the Trp2 binding pocket and the adhesive arm residues . - pubmed
In contrast , AccMGST1 expression was inhibited when the antioxidant vitamin C ( VC ) was fed to experimental bees . - pubmed
Bromodomain and extraterminal domain inhibition synergizes with WEE1 - inhibitor AZD1775 effect by impairing nonhomologous end joining and enhancing DNA damage in nonsmall cell lung cancer . - pubmed
AZD1775 , an inhibitor of the G2 - M cell cycle checkpoint kinase WEE1 , induces DNA damage by promoting premature mitotic entry . - pubmed
Thus , we hypothesized that BET inhibition would increase AZD1775 - induced cytotoxicity by impairing DNA damage repair . - pubmed
Two BET inhibitors , JQ1 and AZD5153 , increased and prolonged AZD1775 - induced DNA double - strand breaks ( DSBs ) and concomitantly repressed genes related to nonhomologous end joining ( NHEJ ) , including XRCC4 and SHLD1 . - pubmed
The objective of this study was to determine if combination of polymyxin ( polymyxin B or colistin ) with a nonantibiotic small molecule AR-12 , a celecoxib derivative that is devoid of cyclooxygenase 2 ( COX-2 ) inhibitory activities , can be an effective strategy against polymyxin - resistant MDR bacteria . - pubmed
While NT - proBNP levels did not change significantly under treatment ( 2561 + - 2042 ng / l vs. 1938 + - 1524 ng / l , p = 0.20 ) , creatinine levels increased ( 1.14 + - 0.52 mg / dl vs. 1.35 + - 0.74 mg / dl , p = 0.002 ) . - pubmed
Estrogen induction upregulated the expression of GPAM and AGPAT2 , downregulated the expression of AGPAT3 , AGPAT9 , LPIN1 and LPIN2 , and had no effect on the expression of the other genes . - pubmed
This family comprises six members three of which , GATA4 , 5 , and 6 , are predominantly expressed in cardiac cells where they activate specific downstream gene targets via interactions with one another and with other TFs and signaling molecules . - pubmed
Glucose - Sensitive Nanoparticles Based On Poly ( 3 - Acrylamidophenylboronic Acid - Block - N - Vinylcaprolactam ) For Insulin Delivery . - pubmed
Background : Compared with random copolymers , block copolymerization is easier to prepare for nanoparticles with core - shell structure , and they will have better glucose sensitivity and higher insulin loading . - pubmed
Purpose : In our study , insulin - loaded poly ( 3 - acrylamidophenylboronic acid - block - N - vinyl caprolactam ) p ( AAPBA - b - NVCL ) nanoparticles were successfully prepared and were glucose - sensitive , which could effectively lower the blood sugar levels within 72 hrs . - pubmed
Meanwhile , insulin can be easily loaded by p ( AAPBA - b - NVCL ) nanoparticles and an effective sustained release of insulin was observed when the nanoparticles were placed in physiological saline . - pubmed
Conclusion : Our research suggested that these p ( AAPBA - b - NVCL ) nanoparticles might have the potential to be applied in a delivery system for insulin or other hypoglycemic proteins . - pubmed
Effect of pemafibrate , a novel selective peroxisome proliferator - activated receptor - alpha modulator ( SPPARMalpha ) , on urinary protein excretion in IgA nephropathy with hypertriglyceridemia . - pubmed
Pemafibrate is a novel selective peroxisome proliferator - activated receptor - alpha modulator and may be distinct from conventional fibrates in terms of its pharmacological activity and hepatic and renal safety . - pubmed
METHODS : This was a retrospective study of adult commercial and Medicare Advantage with Part D ( MAPD ) enrollees with at least one claim for a PAH - related medication from January 01 , 2012 to March 31 , 2017 , at least one medical claim with a pulmonary hypertension diagnosis , and continuous health plan enrollment at least 6 months prior to and at least 12 months following the date of the first pharmacy claim for PAH - related therapy ( index date ) . - pubmed
From EELS measurements performed for 99.99 % Al , Si wafer and 99.99 % Fe , linear relationships were confirmed between the thickness of the TEM thin foil and the ratio of the total intensity of EELS spectrum to the total intensity of zero - loss spectrum for all samples . - pubmed
The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO ( 2 ) incubator . - pubmed
Functional changes ( acceleration of cell migration ) after AMC exposure were statistically significant for the MSC samples only . - pubmed
Alteration of cell motility dynamics through collagen fiber density in photopolymerized polyethylene glycol hydrogels . - pubmed
We demonstrated a method to encapsulate collagen into PEG hydrogel crosslinked via photopolymerization and studied the effect of fiber density on motility dynamics . - pubmed
The changes in [ Ca2+]cys induced by HCO3 - could be detected by an aequorin - based calcium imaging system . - pubmed
In vitro stability of PDMS - SB - UU was confirmed against lipase and 30 % H2O2 for 8 weeks , and PDMS - SB - UU demonstrated significantly higher resistance to fibrinogen adsorption and platelet deposition compared to control PDMS . - pubmed
After the treatment period , the histopathological examination of nasal and sinus mucosa was performed and levels of cytokines ( tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1beta ( IL-1beta ) , interleukin-6 ( IL-6 ) ) and oxidative stress in the blood , nasal mucosa , lungs and brain were also analyzed . - pubmed
Quercetin administration diminished the exudate and degree of inflammation in lamina propria of nasal and sinusal areas , parallel with the decreased secretion of TNF - alpha ( 40.2 % reduction after the low dose of LPS , and 35.4 % reduction after the high dose of LPS ) and IL-6 ( 21.4 % reduction after the low dose of LPS and 35.8 % reduction after the high dose of LPS ) . - pubmed
In lungs , quercetin reduced TNF - alpha ( 43.3 % ) and IL-6 levels ( 24.5 % ) , and in the brain , the protective effect was noticed only on TNF - alpha ( 46.5 % ) . - pubmed
Genes that encoding the key enzyme in carbon - related metabolism such as Rubisco , AMY , PK , ME , CS , ACAT , KAS , and DGAT were identified . - pubmed
Cellular and molecular features of EDC exposure : consequences for the GnRH network . - pubmed
This observation , together with our previous finding that mutant strains defective in acyl - CoA synthetase I can not incorporate exogenous fatty acid into cellular lipids but are able to degrade fatty acid via beta - oxidation , indicates the presence of two functionally distinct long - chain acyl - CoA pools in the cell -- i.e. - pubmed
Unlike the wild - type and the revertant strains as well as the mutants lacking acyl - CoA synthetase II , the mutants defective in acyl - CoA synthetase I do not exhibit the repression of acetyl - CoA carboxylase [ acetyl - CoA : carbon - dioxide ligase ( ADP - forming ) , EC 6.4.1.2 ] by exogenous fatty acid . - pubmed
Measurement of the two long - chain acyl - CoA pools with the aid of appropriate mutant strains has indicated that the long - chain acyl - CoA to be utilized for lipid synthesis , but not that to be degraded via beta - oxidation , is involved in the repression of acetyl - CoA carboxylase . - pubmed
Refolding and reactivation of liver alcohol dehydrogenase after dissociation and denaturation in 6 M guanidine hydrochloride . - pubmed
Horse - liver alcohol dehydrogenase has been dissociated and denatured by 6 M guanidinium hydrochloride . - pubmed
The concentrations of the enzyme , as well as the coenzyme ( NAD + ) , and Zn2 + , affect the reactivation significantly , since high concentrations promote the formation of inactive aggregation products . - pubmed
injection of either clorgyline ( 0.5 , 1 or 5 mg / kg ) which selectively inhibits Type A MAO or deprenyl ( 0.5 , 5 or 10 mg / kg ) which selectively inhibits Type B MAO . - pubmed
Glucoprivation produced by 0.5 - -2 U / kg insulin did not stimulate food intake although plasma glucose fell to a mean of 49 mg/100 ml ( 43 % of control ) after 1 U / kg insulin . - pubmed
Pharmacological blockade of the action of glutamate at the N - methyl - D - aspartate ( NMDA ) receptor , ( the glutamate receptor subtype principally involved in the neurotoxic effects of the amino acid ) provides a novel therapeutic approach to cerebral ischaemia . - pubmed
Recoverin is a recently discovered 26 kDa calcium - binding protein , which activates guanylate cyclase in retinal photoreceptors when the intracellular concentration of free calcium drops upon photoexcitation . - pubmed
Infusion of ANF caused a progressive fall of plasma aldosterone and plasma renin activity . - pubmed
Intrathecal perfusion with 50 and 500 microM 5 - HT also released a nucleotide which eluted from the HPLC column at a retention time identical to that of cyclic AMP standards , and was reduced following incubation with cyclic AMP phosphodiesterase . - pubmed
At P2 and at P7 , concentrations of electron - dense material were observed in 0.5 - 0.7 % of the axon profiles in the medullary PT . - pubmed
Our results show that a decondensed state of the NOR chromatin is necessary but not sufficient to permit silver impregnation , allowing us to conclude that transcriptional NOR activity in the previous interphase is additionally required for silver staining . - pubmed
Experiments were designed to analyze the effects of endothelium - derived relaxing factor(s ) ( EDRF ; released basally or on stimulation with acetylcholine ) and nitric oxide ( NO ) on smooth muscle of coronary arteries of different diameter . - pubmed
Because of similar T2 NMR relaxation times , the water was doped with a paramagnetic ion , Mn+2 , to reduce its T2 relaxation time . - pubmed
Is phospholipid a required cofactor for the activity of mammalian signal peptidase ? - pubmed
We have used the calmodulin antagonists , trifluoperazine ( TFP ) and calmidazolium , to study the potential role of this protein in the movement of acetylcholine receptors ( AChRs ) to and from the myotube membrane , as well as in the formation of clusters of AChRs within the plasma membrane . - pubmed
An enantioselective high - performance liquid chromatographic method for chloroquine and desethylchloroquine was developed using a chiral alpha 1 - acid glycoprotein column . - pubmed
[ Relation between optic absorption spectrum and structure of nitrosyl hemoglobin ] . - pubmed
Norepinephrine and the post - ejaculatory refractory period . - pubmed
Administration of norepinephrine synthesis inhibitors significantly increased both mount and intromission latencies and caused a dramatic increase in the length of the post - ejaculatory refractory period . - pubmed
, by the addition of decoyinine , a specific inhibitor of GMP synthetase . - pubmed
Therefore , we studied the effect of xenon inhalation on the CT appearance of five demyelinated plaques . - pubmed
Therefore , xenon enhancement does not generally increase the sensitivity of CT for detecting cerebral demyelination . - pubmed
The serological diagnosis of virus hepatitis A and B depends on the demonstration of the various virus antigens ( HA - antigen , HBs - antigen , HBe - antigen ) and on their corresponding antibodies ( HA- , IgG- and IgM - antibody , HBc - antibody , Hbe - antibody and HBs - antibody ) . - pubmed
Our observations suggest that vitamin C stimulates the elaboration of Ig M - type antibodies in particular . - pubmed
Sugar meal analysis , using high - performance anion exchange ( HPAE ) chromatography with pulsed amperometric detection ( PAD ) and the cold anthrone test , was used to demonstrate that significantly more sandflies fed on bean plants contaminated with aphids and honeydew than on uncontaminated beans . - pubmed
Single - amino - acid substitutions were made at several positions within the DNA binding domain of p53 , and this set of p53 point mutants were tested with DNA site variants for DNA binding . - pubmed
Interestingly , mutant Cys-277 to Gln bound a consensus site as two and four monomers , as opposed to the wild - type p53 fragment , which invariably binds this site as four monomers . - pubmed
Stimulation of heparinized blood with 1 microM formyl - methionyl - leucyl - phenylalanine ( FMLP ) resulted in the formation of & lt ; 30 pmol / ml plasma of 5 - lipoxygenase ( 5 - LO ) products . - pubmed
The priming effect of LPS was also observed using the complement fragment C5a or interleukin 8 as agonists . - pubmed
Treatment of PMN with LPS strongly enhanced the release of arachidonic acid after stimulation with FMLP . - pubmed
Involvement of ryanodine receptors in sphingosylphosphorylcholine - induced calcium release from brain microsomes . - pubmed
SPC has little effect on [ 3H ] IP3 binding but enhances [ 3H ] ryanodine binding , as expected for an activator of ryanodine receptors . - pubmed
SPC - induced Ca2 + release is inhibited by ryanodine receptor blockers but not by selective blockers of IP3 receptors . - pubmed
We conclude that SPC releases Ca2 + from brain microsomes by activating ryanodine receptors rather than IP3 receptors . - pubmed
Activation of an additional SPC - sensitive pathway for releasing Ca2 + is not precluded . - pubmed
In this report , from the nucleotide sequence , we demonstrate that leukocidin and gamma - hemolysin share the F or H gamma I component and the specificity of both toxins depends on this component . - pubmed
A common target of FK506 and cyclosporin A is inhibition of activation of the NFAT transcription factor , for which a specific binding region is present in the promoter of the IL-2 gene . - pubmed
The isolation protocol included tissue homogenization in 0.4 M HClO4 , centrifugation , solid - phase extraction through LiChroprep RP-18 material , chromatofocusing on polybuffer exchanger PBE 94 - modified Sepharose and dialysis against water . - pubmed
The amino terminus of mammalian nucleolin specifically recognizes SV40 T - antigen type nuclear localization sequences . - pubmed
coli D - glyceraldehyde-3 - phosphate dehydrogenase modified by 2,3 - butanedione : manifestation of a pairwise of non - equivalence of active centers . - pubmed
coli d - glyceraldehyde-3 - phosphate dehydrogenase by an arginine - specific reagent , 2,3 - butanedione , stabilized the tetrametric enzyme in an asymmetric state , with only two of the four active centers able to catalyze oxidative phosphorylation of D - glyceraldehyde-3 - phosphate . - pubmed
Analogous results have been previously obtained with the rabbit muscle D - glyceraldehyde dehydrogenase modified at a single arginine residue per subunit ( Kuzminskaya , E.V. - pubmed
coli enzyme , activity loss upon arginine modification never exceeded 80 - 82 % . - pubmed
Determination of phosphatidylserine ( PS ) in the outer leaflet of RBC by the PS - dependent prothrombinase assay revealed that PS redistributed to the cell 's outer leaflet upon in vitro storage and vesiculation . - pubmed
Inhibition of PS movement by oxidation of membrane sulfhydryls with pyridyldithioethylamine resulted in higher prothrombinase levels and enhanced clearance of vesiculated RBC . - pubmed
When different categories of obesity are compared , blood pressure and one or more of the lipids show an increasing trend but glucose or insulin level shows no significant change . - pubmed
Even where a relationship exists , as with triglyceride and HDL cholesterol levels , the magnitude of the response is lower among the Inuit . - pubmed
These advantages are related to high affinity at the 5 - HT2 binding site , no association with an alteration in dopamine A9 firing rates , and lower D2 striatal receptor blockade of olanzapine . - pubmed
Allene oxide synthase ( AOS ; hydroperoxide dehydratase ; EC 4.2.1.92 ) catalyzes the first step in the biosynthesis of jasmonic acid from lipoxygenase - derived hydroperoxides of free fatty acids . - pubmed
AOS was induced in leaves by wounding , systemin , 12 - oxophytodienoic acid , and methyl jasmonate . - pubmed
Salicylic acid inhibited AOS and PIN II expression , and the addition of 12 - oxophytodienoic acid or methyl jasmonate did not prevent the inhibition of PIN II expression in the presence of salicylic acid . - pubmed
Ethylene induced the expression of AOS , but the presence of ethylene alone did not produce an optimal induction of PIN II . - pubmed
The addition of silver thiosulfate , an ethylene action inhibitor , prevented the wound - induced expression of both AOS and PIN II . - pubmed
In the light and in the presence of organic compounds , strain OS - H1 oxidized sulfide to elemental sulfur and polysulfides , but was not capable of photoautotrophic growth . - pubmed
Wnt-7a , expressed in the dorsal ectoderm , encodes a key molecule implicated in these events . - pubmed
Human chorionic gonadotropin or an implant containing a gonadotropin - releasing hormone analog was used to initiate follicular and oocyte maturation . - pubmed
Reprogramming of gene expression in cultured cardiomyocytes and in explanted hearts by the myosin ATPase inhibitor butanedione monoxime . - pubmed
BACKGROUND : Butanedione monoxime ( BDM ) is a reversible myosin ATPase inhibitor . - pubmed
Accumulation of catabolic intermediates was investigated with three classes of Tn5 mutants with the following polycyclic aromatic hydrocarbon ( PAH)-negative phenotypes ; ( class I NAP(- ) PHE(- ) FLA(- ) , class II NAP(- ) PHE(- ) , and class III FLA(- ) ) . - pubmed
Mutant 200pbhA and other class I mutants lost the ability to catalyze the initial dihydroxylation step and did not transform NAP , ANT , PHE , or FLA . - pubmed
When mutant 132pbhC was incubated with 1 - hydroxy-2 - naphthoic acid , it accumulated trans - o - hydroxybenzylidene pyruvate . - pubmed
Acenaphthenone was oxidized via incorporation of one molecule of dioxygen by another oxygenase . - pubmed
Gonadotropin - releasing hormone ( GnRH)-antagonists can suppress the pituitary hormone secretion completely within a few hours , allowing the avoidance of premature luteinization within controlled ovarian hyperstimulation ( COH ) for assisted reproductive technologies ( ART ) by midcycle administration . - pubmed
Two different protocols were described , which were widely used in COH in several phase II and III studies as well as in clinical practice since the GnRH - antagonists Cetrorelix ( Cetrotidesound recording copyright sign ; Serono International S.A. , Geneva , Switzerland ) and Ganirelix ( Orgalutansound recording copyright sign , Antagonsound recording copyright sign ; Organon , Oss , The Netherlands ) are available on the market . - pubmed
Estradiol secretion is not compromised by the GnRH - antagonists using recombinant follicle - stimulating hormone ( FSH ) for COH . - pubmed
The incidence of a premature leutinizing hormone ( LH ) surge is far below 2 % while the pituitary response remains preserved , allowing the induction of ovulation by GnRH or GnRH - agonists . - pubmed
The two ionized forms of ascorbate present in aqueous solution reduce SNP in the order A2->>HA->>H2A ( H2A = L - ascorbic acid ) to release NO . - pubmed
In the frontal cortex , regional metabolic glucose response to m - CPP was entered into a group ( impulsive aggressive , control ) x slice ( dorsal , middle , orbital ) x position ( medial , lateral ) x location ( anterior , posterior ) x hemisphere ( right , left ) mixed - factorial analysis of variance design . - pubmed
While estrogen has been shown to induce osteoblastic OPG production , the effects of androgens on OPG production have not been defined . - pubmed
Inhibition of OPG mRNA levels and protein production by 5alpha - DHT was completely abrogated by the AR antagonist , hydroxyflutamide ( OHF ) , indicating that these effects are directly mediated by the AR . - pubmed
Of note , OHF alone increased OPG mRNA levels and protein secretion by 2- to 3 - fold . - pubmed
Moreover , 5alpha - DHT and testosterone also decreased OPG protein secretion by 40 - 46 % in the untransformed MSC cells , while OHF stimulated it . - pubmed
In conclusion , we demonstrate that androgens specifically inhibit OPG mRNA levels and protein secretion by osteoblastic cells . - pubmed
RESULTS : Most pronounced BR to histamine was observed in allergic asthmatics ( n = 28 ) , irrespective of the presence or absence of allergic rhinitis . - pubmed
The time - course accumulation of the inositol phosphate in response to GnRH revealed that bfGnRHR-1 and - 2 were rapidly desensitized , whereas bfGnRHR-3 was slowly desensitized . - pubmed
These results , therefore , suggest that differential regulatory mechanisms for desensitization and internalization of the GnRHR are involved in diverse cellular and physiological responses to GnRH stimulation . - pubmed
The enzyme showed specificity for adenosine and exhibited Michaelis - Menten Kinetics with kappa(cat ) of 1112.41 min(-1 ) and K(m ) of 14.7 microM at pH 7.5 . - pubmed
Purine riboside inhibited the enzyme competitively with K(i ) of 16 microM. - pubmed
Antidepressants , lithium , and valproate indirectly regulate a number of factors involved in cell survival pathways , including CREB , BDNF , Bcl-2 , and MAP kinases , and may thus bring about some of their delayed long term beneficial effects via underappreciated neurotrophic effects . - pubmed
Dopamine released during bursts feeds back onto D ( 2 ) autoreceptors that depress neuronal activity . - pubmed
New findings from Ingram and colleagues suggest that , by contrast , tonic activity excites these neurons by activating an uncoupled Cl- conductance that is mediated by the dopamine uptake transporter . - pubmed
These 16 genes , nine ( IFN inducible protein 9 - 27 , MAA A32 , CCLB protein , integrin beta4 , XRCC1 , K8 , K18 , MT3 , MAPKK6 ) of which were altered in a chemical - specific manner and seven ( MIC1 , bikunin , MTS1 , BMP4 , RAD23A , DOC2 , vimentin ) of which were commonly affected by the MNNG and As or mixture treatments , were examined for expression in detail by real - time RT - PCR . - pubmed
Although OCPs were detected after culturing for a further 5 days ( phase II ) , the reduction of OCPs in phase I was maintained in phase II . - pubmed
Lithium interaction with the cyclooxygenase 2 inhibitors rofecoxib and celecoxib and other nonsteroidal anti - inflammatory drugs . - pubmed
We sought to determine whether NSAIDs that selectively inhibit cyclooxygenase ( COX ) 2 also elevate serum lithium concentrations . - pubmed
METHOD : The U.S. Food and Drug Administration 's Adverse Event Reporting System ( AERS ) database was searched in January 2003 for reports of interactions between lithium and rofecoxib or celecoxib , the selective COX-2 inhibitors marketed in the United States . - pubmed
RESULTS : Eighteen cases of increased serum lithium concentrations after the addition of one of the COX-2 inhibitors to stable lithium therapy were retrieved from AERS , 13 with rofecoxib and 5 with celecoxib . - pubmed
Mutants deficient in glucose-6 - phosphate dehydrogenase and 6 - phosphogluconate dehydrogenase were constructed by disrupting the zwf and gnd genes and were grown in minimal media with two different carbon sources , such as glucose or pyruvate . - pubmed
The measurement of enzyme activities implied a significant change in metabolism , when alternative pathways such as the Entner - Doudoroff pathway ( EDP ) and the malic enzyme pathway were activated in the gnd mutant grown on glucose . - pubmed
As compared with the parent strain , the activities of phosphoglucose isomerase were increased in mutants grown on glucose but decreased in mutants grown on pyruvate . - pubmed
AIM : To compare costs of treatment with risperidone or olanzapine in a naturalistic setting . - pubmed
Neutrophil serine proteinases inactivate surfactant protein D by cleaving within a conserved subregion of the carbohydrate recognition domain . - pubmed
The NSPs generated similar , relatively stable , disulfide cross - linked immunoreactive fragments of approximately 35 kDa ( reduced ) , and sequencing of a major catheptic fragment definitively localized the major sites of cleavage to a highly conserved subregion of the carbohydrate recognition domain . - pubmed
Chemical double mutant cycles have been used to quantify the interactions of halogens with the faces of aromatic rings in chloroform . - pubmed
The halogens are forced over the face of an aromatic ring by an array of hydrogen - bonding interactions that lock the complexes in a single , well - defined conformation . - pubmed
Erythrocyte glutathione and serum total glutathione - S - transferase in epilepsy . - pubmed
Regionally , NAA / Cho was lowered in the internal capsule ( AMN 1.30 + /- 0.20 vs controls 1.69 + /- 0.37 ; p = 0.002 ) and in parieto - occipital white matter ( AMN 1.45 + /- 0.19 vs controls 1.78 + /- 0.55 ; p = 0.04 ) . - pubmed
NAA / Cr was lowered in parieto - occipital white matter ( AMN 2.34 + /- 0.31 vs controls 2.83 + /- 0.71 ; p = 0.04 ) . - pubmed
Serum concentrations of lipids , glucose , insulin , total testosterone ( TT ) , SHBG , estradiol ( E2 ) , and dehydroepiandrosterone sulfate ( DHEA - S ) were measured . - pubmed
Bioavailable testosterone ( BT ) was calculated using TT and SHBG . - pubmed
The metabolic syndrome was defined according to the National Cholesterol Education Program definition , and insulin sensitivity was calculated by use of the quantitative insulin sensitivity check index . - pubmed
RESULTS : Multiple logistic regression analyses showed an inverse relationship according to 1 sd increase for circulating TT [ odds ratio ( OR ) = 0.43 ; 95 % confidence interval ( CI ) , 0.32 - 0.59 ] , BT ( OR = 0.62 ; 95 % CI , 0.46 - 0.83 ) , SHBG ( OR = 0.46 ; 95 % CI , 0.33 - 0.64 ) , and DHEA - S ( OR = 0.76 ; 95 % CI , 0.56 - 1.02 ) with the metabolic syndrome . - pubmed
Linear regression analyses showed that higher TT , BT , and SHBG levels were related to higher insulin sensitivity ; beta - coefficients ( 95 % CI ) were 0.011 ( 0.008 - 0.015 ) , 0.005 ( 0.001 - 0.009 ) , and 0.013 ( 0.010 - 0.017 ) , respectively , whereas no effects were found for DHEA - S and E2 . - pubmed
CONCLUSIONS : Higher testosterone and SHBG levels in aging males are independently associated with a higher insulin sensitivity and a reduced risk of the metabolic syndrome , independent of insulin levels and body composition measurements , suggesting that these hormones may protect against the development of metabolic syndrome . - pubmed
The anticonvulsant potency of the competitive antagonist of the N - methyl - D - aspartate ( NMDA ) receptor , 2 - amino-7 - phosphonoheptanoic acid ( APH ) was investigated in the model of epilepsy induced by systemic administration of metaphit ( 1 -( 1 -( 3 - isothiocyanatophenyl )- cyclohexyl )- piperidine ) . - pubmed
Somatostatin analogues can be useful in reducing gastric acid hypersecretion , serum gastrin and gastric enterochromaffin - like cells , and can thus contribute to treating the disease more effectively . - pubmed
PBDEs and BB 153 were quantified using a gas chromatograph coupled with a mass spectrometer ( MS ) operated in electron - capture negative ionization mode . - pubmed
Intracellular glutathione ( GSH ) , mitochondrial membrane potential ( MMP ) , cell viability ( propidium iodide uptake , PI ) , surface phosphatidylserine ( Annexin V ) , T - lymphocyte ( CD3 , CD4 , CD8 ) , and B - lymphocyte ( CD19 ) phenotype distribution and number were assessed . - pubmed
Regardless of training status , an acute bout of strenuous exercise decreased the total and phenotype specific ( CD3 , CD4 , CD8 ) number of cells , MMP , and GSH levels immediately after exercise . - pubmed
Carbachol ( CCh)-induced desensitization to CCh was interrupted by a transient resensitization during its early stage , with concomitant changes at the muscarinic receptor / G - protein level in smooth muscle of guinea - pig taenia caeci . - pubmed
Surface tension , fluorescence , and circular dichroism ( CD ) methods have been used to investigate the interaction between cationic gemini surfactant 1,2 - ethane bis ( dimethyldodecylammonium bromide ) ( C12C2C12 ) and proteins including bovine serum albumin ( BSA ) and gelatin . - pubmed
Addition of C12C2C12 has a different effect not only on the polarity of the microenvironment in BSA and gelatin systems but also on their fluorescence spectra . - pubmed
It can be seen from far - UV CD spectra that the alpha - helical network of BSA is disrupted and its content decreases from 41.7 % to 27.6 % while the random coil content of gelatin increases from 53.0 % to 55.9 % with increasing C12C2C12 concentration . - pubmed
The results from near - UV CD spectra show that the binding of C12C2C12 induces changes of the microenvironment around the aromatic amino acid residues and disulfide bonds of BSA at high C12C2C12 concentrations . - pubmed
Crystals of the human immunodeficiency virus 1 ( HIV-1 ) subtype C protease ( PR ) complexed with the clinically used inhibitors indinavir ( IDV ) and nelfinavir ( NFV ) have been grown in the monoclinic space group P2(1 ) , with mean unit - cell parameters a = 46.7 ( + /-0.1 ) , b = 59.8 ( + /-0.3 ) , c = 87.0 ( + /-0.4 ) A , beta = 95.2 ( + /-0.5 ) degrees . - pubmed
The DNA methylation state of 11 CpG sites of surfactant protein ( SP )- A1 , - B , - C , and - D was determined using universal bead arrays . - pubmed
Normal lung tissues with a higher level of unmethylated SP - A1_1468 and SP - D_1170 CpG exhibited a higher level of SP - A1 and SP - D gene transcripts indicating that CpG methylation may play a role in gene expression . - pubmed
Both NAC and WY14643 , a peroxisome proliferator - activated receptor ( PPAR )- alpha agonist attenuated these LCM - induced effects in OL - progenitors . - pubmed
Similar to WY14643 , NAC attenuated LCM - induced inhibition of PPAR - alpha activity in developing OLs . - pubmed
Studies conducted with cytokines and diamide ( a thiol - depleting agent ) confirmed that cytokines are active agents in LCM which may be responsible for inhibition of OL development via peroxisomal dysfunction and induction of oxidative stress . - pubmed
Collectively , these data provide evidence that the modulation of PPAR - alpha activity , thus peroxisomal function by NAC attenuates LPS - induced glial factors - mediated inhibition of OL development suggesting new therapeutic interventions to prevent the devastating effects of maternal infections . - pubmed
Involvement of lysine 1047 in type I collagen - mediated activation of polymorphonuclear neutrophils . - pubmed
post - translational binding of cyanate to lysine epsilon - NH ( 2 ) groups ) impairs PMN oxidative functions , suggesting the potential involvement of lysine residues in this process . - pubmed
The present study was devoted to the identification of lysine residues involved in the collagen - induced activation of PMNs . - pubmed
The inhibition of PMN activation by collagen in the presence of 6 - amino - hexanoic acid , a structural analogue of lysine residues , confirmed the involvement of specific lysine residues . - pubmed
Modification of lysine residues by carbamylation demonstrated that only one residue , located within the alpha ( 1 ) CB6 collagen peptide , was involved in this mechanism . - pubmed
A recombinant alpha(1)CB6 peptide , designed for the substitution of lysine 1047 by glycine , exhibited decreased activity , demonstrating that the lysine residue at position 1047 within the collagen molecule played a significant role in the mechanism of activation . - pubmed
These results help to understand in more detail the collagen - mediated PMN activation mechanism and confirm the prominent involvement of lysine residues in interactions between extracellular matrix proteins and inflammatory cells . - pubmed
Help from CD4 T cells may be required for optimal generation and maintenance of memory CD8 T cells and also for optimal Ag reactivation . - pubmed
We examined whether the helper cell and the CD8 killer cell need to have the same Ag specificity for help to be effective during interactions of memory T cells with mature APC . - pubmed
Results were as follows for B ( 10(-9 ) K cm(3 ) s(-1 ) ) , E(a ) ( K ) , and k(298 ) ( 10(-12 ) cm(3 ) s(-1 ) ): cyclohexane , 3.24 + /- 0.14 , 332 + /- 12 , 7.13 ; cyclo - octane , 3.47 + /- 0.30 , 149 + /- 26 , 14.1 ; 2 - methylhexane , 1.45 + /- 0.08 , 110 + /- 15 , 6.72 ; 3 - methylhexane , 1.50 + /- 0.08 , 128 + /- 16 , 6.54 ; methylcyclopentane , 1.65 + /- 0.07 , 109 + /- 13 , 7.65 ; methylcyclohexane , 1.86 + /- 0.09 , 83 + /- 14 , 9.43 ; methylcycloheptane , 3.45 + /- 0.45 , 142 + /- 36 , 14.4 ; n - propylcyclohexane , 2.83 + /- 0.14 , 112 + /- 15 , 13.0 ; isopropylcyclohexane , 1.79 + /- 0.11 , -44 + /- 34 , 13.9 . - pubmed
However , the LTF avoids a distant donor site , provides morphologically similar donor tendon that is readily accessible , and avoids morbidity that may be associated with the use of DTG . - pubmed
Effect of erythromycin A and its new derivative EM201 on type I collagen production by cultured dermal fibroblasts . - pubmed
In this study , we investigated the effects of erythromycin A ( EMA ) and its new derivative EM201 on type I collagen production by cultured dermal fibroblasts . - pubmed
Dermal fibroblasts were cultured with 10(-9 ) M-10(-5 ) M EMA or EM201 , and collagen production was measured by incubation with radioactive proline , SDS - polyacrylamide gel electrophoresis and fluorography . - pubmed
The results showed that both EMA and EM201 increased collagen production and type I collagen mRNA level ( to a maximum of 200 % with EMA and 250 % with EM201 ) in a dose - dependent manner in cultured dermal fibroblasts . - pubmed
Transcription of the type I collagen gene was also increased by both macrolides . - pubmed
Structural basis of cyanide inhibition of Ni , Fe - containing carbon monoxide dehydrogenase . - pubmed
Carbon monoxide dehydrogenases ( CODHs ) catalyze the reversible oxidation of carbon monoxide with water to carbon dioxide , two protons , and two electrons . - pubmed
The CODHs of anaerobic microorganisms harbor a complex Ni / Fe / S - containing metal center called a C - cluster in their active site , which activates the substrates water and carbon monoxide , stabilizes an intermediary metal - carboxylate , and transiently stores the two electrons generated in the reaction . - pubmed
Several small molecules have been reported to inhibit carbon monoxide oxidation by CODHs , among which the cyanide anion acts as a slow binding inhibitor . - pubmed
Tetrahydroxy stilbene glucoside reduces the cognitive impairment and overexpression of amyloid precursor protein induced by aluminum exposure . - pubmed
The effect of TSG was evaluated by passive avoidance task , and APP expression was evaluated by Western blotting . - pubmed
Both TSG and VE significantly ameliorated the performance impairment in the passive avoidance task , and suppressed the over - expression of APP . - pubmed
c - Jun activation is required for 4 - hydroxytamoxifen - induced cell death in breast cancer cells . - pubmed
The c - Jun N - terminal kinase ( JNK ) has been shown to mediate tamoxifen - induced apoptosis in breast cancer cells . - pubmed
However , the downstream mediators of the JNK pathway linking tamoxifen to effectors of apoptosis have yet to be identified . - pubmed
We show that before DNA fragmentation and caspase 3 / 7 activation , cytotoxic concentrations of 4 - hydroxytamoxifen ( OHT ) induced JNK - dependent phosphorylation of c - Jun at JNK sites earlier shown to regulate c - Jun - mediated apoptosis . - pubmed
In addition , OHT induced ERK - dependent expression of c - Fos and transactivation of an AP-1 - responsive promoter . - pubmed
In particular , the ectopic expression of dominant - negative constructs blocking either AP-1 activity or c - Jun N - terminal phosphorylation prevented DNA fragmentation after OHT treatment . - pubmed
Taken together , our results indicate that the c - Jun / c - Fos AP-1 complex has a pro - apoptotic role in OHT - treated cancer cells and suggest that pharmacological boosts of c - Jun activation may be useful in a combination therapy setting to sensitize cancer cells to tamoxifen - mediated cell death . - pubmed
Regulation of conductance by the number of fixed positive charges in the intracellular vestibule of the CFTR chloride channel pore . - pubmed
Rapid chloride permeation through the cystic fibrosis transmembrane conductance regulator ( CFTR ) Cl (- ) channel is dependent on the presence of fixed positive charges in the permeation pathway . - pubmed
Thus , the mutant channel K95S / S1141 K showed Cl (- ) conductance and open - channel blocker interactions similar to those of wild - type CFTR , thereby " rescuing " the effects of the charge - neutralizing K95S mutation . - pubmed
Thus , the Cl (- ) channel function of CFTR is maximal with a single fixed positive charge in this part of the inner vestibule of the pore , and increasing the number of such charges to two causes a net decrease in overall Cl (- ) transport through a combination of failure to increase Cl (- ) conductance and increased susceptibility to channel block by cytosolic substances . - pubmed
2 - Amino-3,8 - dimethylimidazo [ 4,5 - f ] quinoxaline ( MeIQx)-induced hepatocarcinogenesis is not enhanced by CYP1A inducers , alpha - and beta - naphthoflavone : relationship to intralobular distribution of CYP1A expression . - pubmed
MeIQx is metabolically activated by CYP1A2 and then N - acetyltransferase ( NAT ) , findings that suggest that its carcinogenic potential might be enhanced by simultaneous exposure to chemical(s ) inducing CYP1A2 . - pubmed
Unexpectedly , no modifying influence of naphthoflavones on MeIQx - induced hepatocarcinogenesis was demonstrated with reference to glutathione S - transferase placental form ( GST - P ) positive foci in the liver , although up - regulation of CYP1A2 was detected on Western blot analysis . - pubmed
This difference in intralobular distribution of CYP1A might be related to the fact that MeIQx hepatocarcinogenesis was not modified by the two CYP1A inducers . - pubmed
Charcoal hemoperfusion ( HP ) was performed for 4 hours . - pubmed
Crizotinib for EML4 - ALK positive lung adenocarcinoma : a hope for the advanced disease ? - pubmed
Further clinical studies are needed in order to assess if crizotinib , an ALK inhibitor , is able to increase the efficacy of the conventional chemotherapy in this disease subset . - pubmed
Urinary excretion of 3HIA - carnitine was determined by liquid chromatography - tandem MS on d 0 , 14 , and 28 ( depletion ) and on d 35 and 50 ( repletion ) . - pubmed
One structure corresponds to a prefusion state , in which ATL molecules in apposing membranes interact through their GTPase domains to form a dimer with the nucleotides bound at the interface . - pubmed
Here , we show that a CD44 variant ( CD44v ) interacts with xCT , a glutamate - cystine transporter , and controls the intracellular level of reduced glutathione ( GSH ) . - pubmed
Here we used the yeast two - hybrid system to demonstrate that FGFRL1 binds with its C - terminal , histidine - rich domain to Spred1 and to other proteins of the Sprouty / Spred family . - pubmed
We report that crystal cells express elevated levels of Sima protein orthologous to mammalian hypoxia - inducible factor - alpha ( Hif - alpha ) even under conditions of normal oxygen availability . - pubmed
ZHER2:477 is a commercially available anti - HER2 affibody . - pubmed
One of these 2 - helix proteins is MUT - DS , which has alpha - helices 1 and 2 bundles , with a disulfide bridge being formed between the two inserted homocysteines ( 10 - 12 ) . - pubmed
The helix conformation of MUT - DS has been shown to be improved with the placement of a disulfide bridge . - pubmed
To explore cell - autonomous functional differences between naive and memory CD8 T cells that are not influenced by differences in the priming environment , an experimental model has been developed in which physiological numbers of both populations of cells were cotransferred into naive hosts before Ag stimulation . - pubmed
Modulation of FoxO1 phosphorylation / acetylation by baicalin during aging . - pubmed
Included is its ability to suppress reactive species ( RS ) producing activity and modulate nuclear factor - kappaB through cellular redox regulation with enhanced thiol ability . - pubmed
In the present study , we attempted to elucidate the molecular modulation of antioxidant baicalin on the insulin - induced FoxO1 inactivation . - pubmed
Our results further reveal that baicalin regulated FoxO1 phosphorylation via PI3K / Akt by insulin and FoxO1 acetylation by the interaction of CBP and SIRT1 , leading to changes in catalase gene expression during aging . - pubmed
TLR2 / MyD88 / NF - kappaB pathway , reactive oxygen species , potassium efflux activates NLRP3 / ASC inflammasome during respiratory syncytial virus infection . - pubmed
Here , we compare the EGFR binding affinities of GE11 , EGF , and their polyethyleneimine - polyethyleneglycol ( PEI - PEG ) conjugates . - pubmed
We found that although GE11 by itself does not exhibit measurable affinity to the EGFR , tethering it to PEI - PEG increases its affinity markedly , and complex formation with polyinosine / cytosine ( polyIC ) further enhances the affinity to the submicromolar range . - pubmed
PolyIC / PPGE11 has a similar strong antitumor effect against EGFR overexpressing tumors in vitro and in vivo , as polyIC / polyethyleneimine - polyetheleneglycol - EGF ( polyIC / PP - EGF ) . - pubmed
BACKGROUND : Intravenous ( IV ) artesunate is the treatment of choice for severe malaria . - pubmed
CONCLUSIONS : Data from the named patient programme demonstrate that IV artesunate is effective and well - tolerated in European travellers lacking immunity . - pubmed
Treatment of IV artesunate should be limited to the period that IV treatment is required and should be followed by a full oral course of an appropriate anti - malarial drug . - pubmed
Zinc - binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template . - pubmed
The zinc - binding domain ( ZBD ) of prokaryotic DNA primases has been postulated to be crucial for recognition of specific sequences in the single - stranded DNA template . - pubmed
The ability of T7 DNA primase to catalyze template - directed oligoribonucleotide synthesis is eliminated by substitution of any five - amino acid residue - long segment within the ZBD . - pubmed
The role of this region in primase function was further investigated by generating a protein library composed of multiple amino acid substitutions for Pro-16 , Asp-18 , and Asn-19 followed by genetic screening for functional proteins . - pubmed
The results suggest that the zinc - binding mode alone is not responsible for sequence recognition , but rather its interaction with the RNA polymerase domain is critical for DNA binding and for sequence recognition . - pubmed
CONCLUSION : Of the compounds studied , only vitamin E and NAC significantly mitigated the effects of oxidative stress on RPE cells . - pubmed
A - type lamins have been shown to interact and sequester activated phospho - ERK1 / 2(pERK1 / 2 ) at the nucleus . - pubmed
In an assay of gap junctional function , Lmna(-/- ) MEFs transferred dye to 60 % fewer partners compared to Lmna(+/+ ) controls . - pubmed
TGF - beta1 enhanced glioma - induced angiogenesis , which was inhibited by JNK inhibitor SP600125 but not p38 MAPK inhibitor SB202190 , ERK inhibitor PD98059 , or PI3 K inhibitor LY294002 , indicating the important role of TGF - beta1 and JNK pathways in this process . - pubmed
Up to the present , lepiridin , recently suspended , and argatroban ( direct thrombin inhibitors ) have been the approved medicines normally used in treatment , administered in parenteral form . - pubmed
Dabigatran , a new anticoagulant medicine that is a direct and reversible thrombin inhibitor , could theoretically be a medicine employed in treating HIT . - pubmed
Our work provides a facile and versatile approach for the design and fabrication of smart intracellular targeted nanovehicles for effective cancer treatment , and opens a new era in the development of biodegradable polyurethanes for next - generation nanodelivery systems . - pubmed
The values of pH , TN , WSC , and the content of NH : - N of composting were 4.58 % , 12.62 % , 49.06 % , and 35.30 % higher than those of the control . - pubmed
Here we report that IKBKE regulates FOXO3a through phosphorylation of FOXO3a - Ser644 . - pubmed
Previous studies have shown that IKBKE directly activates Akt and that Akt inhibits FOXO3a by phosphorylation of Ser32 , Ser253 and Ser315 . - pubmed
, converting 3 serine residues to alanine ) was inhibited by IKBKE . - pubmed
These findings suggest that IKBKE regulates FOXO3a primarily through phosphorylation of SerS644 and that IKBKE exerts its cellular function , at least to some extent , through regulation of FOXO3a . - pubmed
When the inserted amino acid sequences were aligned with the Cap protein sequence , eight core regions in Cap ( ( 50)SRTFGYT(56 ) , ( 62)VRTPSW(67 ) , ( 68)AVDMMR(73 ) , ( 79)FLPPGG(84 ) , ( 86)SNPRSVPF(93 ) , ( 102)KVEFWP(107 ) , ( 119)GSSXXXLDDN(128 ) and ( 229)PPLNP(233 ) ) were identified , three of which ( ( 50)SRTFGYT(56 ) , ( 86)SNPRSVPF(93 ) and ( 102)KVEFWP(107 ) ) for the first time . - pubmed
Therefore , it was surmised that failure of irinotecan may be the result of overexpression of the DNA repair enzyme tyrosyl - DNA phosphodiesterase ( TDP1 ) , which processes topoisomerase I - DNA complexes resulting from topoisomerase I inhibitor treatment . - pubmed
Critically , knockdown of TDP1 or inhibition of poly ( ADP - ribose ) polymerase-1 ( PARP-1 ) , an enzyme in the same complex as TDP1 , sensitized rhabdomyosarcoma cell lines to analogues of irinotecan . - pubmed
BACKGROUND : The Acute Study of Clinical Effectiveness of Nesiritide in Decompensated Heart Failure ( ASCEND - HF ) randomly assigned 7,141 participants to nesiritide or placebo . - pubmed
Recent work implicates a lipid critical for neuronal function , phosphatidylinositol-4,5 - bisphosphate [ PI ( 4,5 ) P2 ] , in Abeta - induced synaptic and behavioral impairments . - pubmed
Synaptojanin 1 ( Synj1 ) , a lipid phosphatase mediating the breakdown of PI ( 4,5 ) P2 , has been shown to play a role in synaptic vesicle recycling and receptor trafficking in neurons . - pubmed
To this end , we developed a screening assay for Synj1 based on detection of inorganic phosphate liberation from a water - soluble , short - chain PI ( 4,5 ) P2 . - pubmed
The assay displayed saturable kinetics and detected Synj1 's substrate preference for PI ( 4,5 ) P2 over PI ( 3,4,5 ) P3 . - pubmed
Sunitinib is a multiple tyrosine kinase receptor inhibitor that is approved for the treatment of metastatic renal cell carcinoma ( RCC ) . - pubmed
Samples of the intestinal tissue were used for histological evaluation and assays for glutathione ( GSH ) levels , malonyldialdehyde ( MDA ) concentration , myeloperoxidase ( MPO ) activity , nitrate and nitrite ( NO3 /NO2 ) concentration and cytokines levels . - pubmed
Additionally , it avoided the consumption of GSH , decreased pro - inflammatory cytokine levels , MDA and NO3 /NO2 concentrations and diminished the MPO activity . - pubmed
CONCLUSIONS : Our results suggest that the PLS fraction has a protective effect against intestinal damage through mechanisms that involve the inhibition of inflammatory cell infiltration , cytokine releasing and lipid peroxidation . - pubmed
Prostacyclin regulates bone growth via the Epac / Rap1 pathway . - pubmed
In growth plate chondrocytes , inhibition of proliferation and ERK activation by NSAIDs is reversed by forskolin , 8 - bromoadenosine , 3 ' , 5 '- cAMP and a prostacyclin analog , iloprost . - pubmed
The inhibition of proliferation and ERK activation by celecoxib is also reversed by 8CPT-2Me - cAMP , an activator of Epac , implicating the small G protein Rap1 in the pathway activated by iloprost . - pubmed
Bloods showed potassium of 1.8 mEq / L ( normal range 3.5 - 5 ) , free T4 of 29.2 pmol / L ( normal range 6.5 - 17 ) and thyroid - stimulating hormone ( TSH ) of & lt;0.01 mIU / L ( normal range 0.35 - 4.94 ) . - pubmed
The first step in caffeine metabolism is mediated for over 95 % by the CYP1A2 isoform of cytochrome P450 . - pubmed
Therefore , CYP1A2 activity is most conveniently measured through the determination of caffeine clearance . - pubmed
, G-259 - T and C-248 - T ) in gyrA leading to Asp-87 - Tyr and Ser-83 - Phe substitutions in GyrA , respectively , were detected . - pubmed
Of all plasmid - mediated quinolone resistance genes tested , only qnrS ( 4.1 % ) and qnrB ( 1.8 % ) were found . - pubmed
ii ) The absence of exATP generation by the enzyme , and the finding that exATP hydrolysis was largely oligomycin - insensitive , are in line in parental cells with the deficit of OSCP and suggest the occurrence of sub - assemblies together evoking more regulation by IF1 . - pubmed
Mitochondrial Lon protease ( Lon ) regulates several mitochondrial functions , and is inhibited by the anticancer molecule triterpenoid 2 - cyano-3 , 12 - dioxooleana-1,9 ( 11 )- dien-28 - oic acid ( CDDO ) , or by its C-28 methyl ester derivative ( CDDO - Me ) . - pubmed
In particular , they : i ) cause depolarization ; ii ) increase mitochondrial ROS , iii ) alter mitochondrial morphology and proteins involved in mitochondrial dynamics ; iv ) affect the levels of Lon and those of aconitase and human transcription factor A , which are targets of Lon activity ; v ) increase level of protein carbonyls in mitochondria ; vi ) lead to intrinsic apoptosis . - pubmed
Concomitantly , farnesol dose - dependently increased glucose uptake and glucose transport protein 4 ( GLUT4 ) expression without affecting cell viability . - pubmed
Furthermore , quantitative real - time polymerase chain reaction and Western blot showed that farnesol increased the mRNA and protein levels of peroxisome proliferator - activated receptor gamma ( PPARgamma ) , a key regulator of adipocyte differentiation , and the mRNA levels of PPARgamma - regulated fatty acid - binding protein 4 and adiponectin ; in contrast , farnesol downregulated Pref-1 gene , a marker of pre - adipocytes . - pubmed
GW9662 ( 10 micromol / L ) , an antagonist of PPARgamma , reversed the effects of farnesol on cellular lipid content , suggesting that PPARgamma signaling pathway may mediate the farnesol effect . - pubmed
Farnesol ( 25 - 75 mumol / L ) did not affect the mRNA level of 3 - hydroxy-3 - methylglutaryl coenzyme A reductase , the rate - limiting enzyme in the mevalonate pathway . - pubmed
Farnesol may be the mevalonate - derived inducer of adipocyte differentiation and potentially an insulin sensitizer via activation of PPARgamma and upregulation of glucose uptake . - pubmed
Hormones , such as estrogen , follicle stimulating hormone , and leptin , transcription factors , such as Runx2 and osterix , and the wnt signaling pathway are discussed in terms of their roles in bone cell differentiation and function . - pubmed
Glutamate treatment for 30 min induced the permeabilization of lysosomal membranes as assessed by acridine orange redistribution and immunofluorescence of cathepsin B in the cytoplasm . - pubmed
Inhibition of glutamate excitotoxicity by the NMDA receptor antagonist MK-801 and the calcium chelator ethylene glycol - bis ( 2 - aminoethylether )- N , N , N ' , N '- tetraacetic acid , rescued lysosomes from permeabilization . - pubmed
The role of calpain and reactive oxygen species ( ROS ) in inducing LMP was also investigated . - pubmed
It has been reported that lysosomal - associated membrane protein 2 ( LAMP2 ) and heat shock protein ( HSP ) 70 are two calpain substrates that promote LMP in cancer cells ; however , it was found that calpains were activated by glutamate , but only LAMP2 was subsequently degraded . - pubmed
Furthermore , LMP was not alleviated by treatment with the calpain inhibitors calpeptin and SJA6017 , which blocked the cleavage of the calpain substrate alpha - fodrin . - pubmed
It was demonstrated that LMP was significantly alleviated by treatment with the antioxidant N - Acetyl - L - cysteine , indicating that LMP involvement in early glutamate excitotoxicity may be mediated partly by ROS rather than calpain activation . - pubmed
Overall , these data shed light on the role of ROS - mediated LMP in early glutamate excitotoxicity . - pubmed
The tumour protein D52 isoform 1 ( PC-1 ) , a member of the tumour protein D52 ( TPD52 ) protein family , is androgen - regulated and prostate - specific expressed . - pubmed
Calcium was estimated in the reagents used for native gel electrophoresis of Neuronal Calcium Sensor ( NCS ) proteins . - pubmed
NCS proteins exhibit calcium - dependent mobility shift in native gels . - pubmed
Methylation status of two CpG islands ( CpG1 and CpG2 ) in promoter region of DJ-1 was explored by bisulfite specific PCR - based sequencing method . - pubmed
RESULTS : CpG sites in these two CpG islands ( CpG1 and CpG2 ) of DJ-1 were unmethylated in both PD and NC group . - pubmed
When comparing first and third admission - day laboratory values , the increase in leukocyte counts and the decrease in CPK , urea , creatinine , aPTT , PT , INR , and hemoglobin levels were significant in nonfatal cases . - pubmed
Extended Spectrum Beta - Lactamases ( ESBL ) provide high level resistance to beta - lactam antibiotics among bacteria . - pubmed
Metformin elicits its therapeutic effects mainly via activation of AMP - activated kinase ( AMPK ) pathway . - pubmed
We measured concentrations of follicle - stimulating hormone ( FSH ) , luteinizing hormone ( LH ) , and total testosterone ( T ) from 234 of their sons at 12 years . - pubmed
A 10 - fold increase in total prenatal SigmaPCBs was associated with a 64.5 % increase ( 95 % CI : 8.6 , 149.0 ) in FSH , primarily driven by non- dioxin - like congeners . - pubmed
Furthermore , we observed a crosstalk between the Nrf2 / HO-1 signaling pathway and the activation of the nuclear factor - kappaB ( NF - kappaB ) transcription factor , since administration of ZnPP IX ( specific inhibitor of HO-1 ) or Nrf2 knockdown using small interfering RNA ( siRNA ) abolished the anti - inflammatory effects induced by CA . - pubmed
Hydrochlorothiazide ( HCTZ ) is a class IV drug according to the Biopharmaceutical Classification System . - pubmed
The optimized formulations , with reduced amount of surfactant , and composed of medium chain triglycerides , Cremophor EL and Transcutol P did not affect the pH or show drug incompatibilities . - pubmed
During the induction of NMDA - receptor - dependent LTP , Ca2 + influx stimulates recruitment of synaptic AMPA ( alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazole propionic acid ) receptors , thereby strengthening synapses . - pubmed
LTP was restored by expression of wild - type Syt7 but not of a Ca2 + -binding - deficient mutant Syt7 . - pubmed
Blocking postsynaptic expression of Syt1 and Syt7 did not impair basal synaptic transmission , reduce levels of synaptic or extrasynaptic AMPA receptors , or alter other AMPA receptor trafficking events . - pubmed
Moreover , expression of dominant - negative mutant Syt1 which inhibits Ca2 + -dependent presynaptic vesicle exocytosis , also blocked Ca2 + -dependent postsynaptic AMPA receptor exocytosis , thereby abolishing LTP . - pubmed
Our results suggest that postsynaptic Syt1 and Syt7 act as redundant Ca2 + -sensors for Ca2 + -dependent exocytosis of AMPA receptors during LTP , and thereby delineate a simple mechanism for the recruitment of AMPA receptors that mediates LTP . - pubmed
The RUN domain Beclin-1 - interacting cysteine - rich - containing ( Rubicon ) protein is involved in the maturation step of autophagy and the endocytic pathway as a Beclin-1 - binding partner , but little is known regarding the role of Rubicon during viral infection . - pubmed
This study reports the successful structure - based design and synthesis of new 7 - azaindenoisoquinolines that act as triple inhibitors of Top1 , Tdp1 , and Tdp2 . - pubmed
Sigma-1 - mediated antihyperalgesia was reversed by the opioid antagonists naloxone and naloxone methiodide ( a peripherally restricted naloxone analog ) and by local administration at the inflamed site of monoclonal antibody 3 - E7 , which recognizes the pan - opioid sequence Tyr - Gly - Gly - Phe at the N terminus of most endogenous opioid peptides ( EOPs ) . - pubmed
Prdx-1 is an anti - apoptotic and stress response protein which protects cells from damage by ROS and H2O2 . - pubmed
Inhibition of the p38 MAPK by a specific inhibitor , SB203580 , abrogates the ESAT-6 mediated induction of Prdx-1 expression as well as the phosphorylation of NRF-2 in a time - dependent manner . - pubmed
The ectoderm had two morphologically and immunocytochemically distinct populations of neurons , the motor nerve net ( MNN ) , which was immunoreactive to the taurine - like molecule , and the diffuse nerve net ( DNN ) , which was immunoreactive to the neuropeptide Phe - Met - Arg - Phe - NH2 ( FMRFamide ) . - pubmed
In conclusion , these results suggest that Nano - C3 G can reduce UVB - induced epidermal damage through the p53 - mediated apoptosis signaling pathway . - pubmed
Nano - encapsulation of epigallocatechin gallate in the ferritin - chitosan double shells : Simulated digestion and absorption evaluation . - pubmed
EGCG - loaded H-2F complex ( EHF ) was firstly prepared by taking advantages of the reversible self - assembly of the ferritin , and the EHF - CS composite ( EHFC ) was fabricated by electrostatic interactions with binding number n of ( 4.1 + - 0.11 ) and binding constant K of ( ( 5.3 + - 0.2 ) x 105 M-1 ) , respectively . - pubmed
It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69 % ( w / w ) . - pubmed
SDS - PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin ; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract . - pubmed
Atomic scale reversible opto - structural switching of few atom luminescent silver clusters confined in LTA zeolites . - pubmed
Luminescent silver clusters ( AgCLs ) stabilized inside partially Ag exchanged Na LTA zeolites show a remarkable reversible on - off switching of their green - yellowish luminescence that is easily tuned by a hydration and dehydration cycle , making them very promising materials for sensing applications . - pubmed
lutzii 's gp43 was not observed in IB or silver / PAS staining . - pubmed
Identification of a functional FADS1 3'UTR variant associated with erythrocyte n-6 polyunsaturated fatty acids levels . - pubmed
BACKGROUND : Blood polyunsaturated fatty acid ( PUFA ) levels are determined by diet and by endogenous synthesis via Delta5 - and Delta6 - desaturases ( encoded by the FADS1 and FADS2 genes , respectively ) . - pubmed
Genome - wide association studies have reported associations between FADS1 - FADS2 polymorphisms and the plasma concentrations of PUFAs , HDL- and LDL - cholesterol , and triglycerides . - pubmed
METHODS : To address this question , we ( 1 ) genotyped individuals ( n = 540 ) for the rs174547 polymorphism to confirm associations with PUFA levels used as surrogate estimates of desaturase activities and ( 2 ) examined the functionality of variants in linkage disequilibrium with rs174547 using bioinformatics and luciferase reporter assays . - pubmed
RESULTS : The rs174547 minor allele was associated with higher erythrocyte levels of dihomo - gamma - linolenic acid and lower levels of arachidonic acid , suggesting a lower Delta5 - desaturase activity . - pubmed
In a second study , we performed a retrospective analysis of patients who underwent transesophageal echocardiography ( TEE ) for various reasons , assigned these to 2 groups based on the heart - rhythm ( sinus - rhythm [ SR ] vs AF ) and examined associations between clinical and echocardiographic parameters and intra - cardiac thrombogenic material . - pubmed
Norepinephrine was modestly increased while the ratio between plasma dihydroxyphenylglycol ( DHPG ) and norepinephrine levels , a marker for norepinephrine clearance , was reduced with UFP + O3 . - pubmed
Both oxazolidinones caused a concentration- and time - dependent inhibition of CYTox I expression , cytochrome c - oxidase activity and mitochondrial spare capacity . - pubmed
While the expression of CYTox I was completely recovered after 6 days of drug washout , we observed only a partial ( linezolid ) or no ( tedizolid ) recovery of cytochrome c - oxidase activity , and no rescue of mitochondrial spare capacity ( after 3 days ) . - pubmed
RESULTS : baseline troponin was 3.00 ( 0.06 ; 5.20 ) ng / mL in the tirofiban group versus 4.6 ( 0.02 ; 13.10 ) ng / mL in the no - tirofiban group ( p=0.229 ) , and post - procedural peak 3.5 ( 0.06 ; 5.50 ) ng / mL versus 6.30 ( 0.09 ; 28.40 ) ng / mL ( p=0.191 ) . - pubmed
Peak - baseline difference in troponin was lower in the tirofiban group than in the no - tirofiban group : 0.3 ( 0.00 ; 1.7 ) ng / mL versus 1.3 ( 0.01 ; 10.00 ) ng / mL ( p=0.044 ) ; the relative peak - baseline change in troponin was analogously different : 24.3 % ( 0 % ; 44.7 % ) versus 50 % ( 21.3 % ; 80.0 % ) ( p=0.039 ) . - pubmed
CONCLUSIONS : Tirofiban during CAS might provide peri - procedural myocardial protection and reduce myocardial injury as determined by serial troponin measurements . - pubmed
Herein , a uniform wafer - scale 1T - WS2 film is prepared using a plasma - enhanced chemical vapor deposition ( PE - CVD ) system . - pubmed
All the Pt ( II ) complexes exhibit admixed 3(LC / MLCT ) characters in T1 states with various proportions , which are strongly structure - dependent . - pubmed
Compared with wild - type , both VX and VP transgenic lines grew better at 200mM NaCl , and also exhibited higher tolerance and faster recovery from water - deficit stress : these performances were associated with more Na + , K+ and Ca2 + accumulation in their leaves and roots , which caused lower leaf solute potential and thus retained more water . - pubmed
FSH , LH , testosterone , semen volume , age , BMI , ethnicity and testicular size on clinical evaluation were included in these models . - pubmed
The values of affinity energy obtained for the hydroxychloroquine ligands was -9.9 kcal / mol and for the chloroquine of -10.8 kcal / mol which indicate that the coupling contributes to an effective improvement of the affinity energies with the protease . - pubmed
Alkaline Phosphatase Activity Is a Key Determinant of Vascular Responsiveness to Norepinephrine . - pubmed
Here , we tested the hypothesis that TNAP ( tissue nonspecific alkaline phosphatase ) modulates vascular responsiveness to norepinephrine . - pubmed
At 100 micromol / L , L - p - BT further reduced mesenteric TNAP activity and caused an additional significant right - shift of the norepinephrine concentration versus vasoconstriction relationship . - pubmed
A higher concentration ( 200 micromol / L ) of L - p - BT had no further effect on either mesenteric TNAP activity or norepinephrine - induced vasoconstriction . - pubmed
Additional experiments showed that the effects of TNAP inhibition on norepinephrine - induced vasoconstriction were not mediated by accumulation of pyrophosphate or ATP ( TNAP substrates ) nor by reduced adenosine levels ( TNAP product ) . - pubmed
TNAP inhibition significantly reduced the Hillslope of the norepinephrine concentration versus vasoconstriction relationship from 1.8 + -0.2 ( consistent with positive cooperativity of alpha1 - adrenoceptor signaling ) to 1.0 + -0.1 ( no cooperativity ) . - pubmed
Selective activation of A1 - adenosine receptors , which are known to participate in coincident signaling with alpha1 - adrenoceptors , reversed the suppressive effects of L - p - BT on norepinephrine - induced vasoconstriction . - pubmed
TNAP modulates vascular responses to norepinephrine likely by affecting positive cooperativity of alpha1 - adrenoceptor signaling via a mechanism involving A1 receptor signaling . - pubmed
Immobilization of aminoacylase by adsorption to tannin immobilized on aminohexyl cellulose . - pubmed
The immobilization of aminoacylase ( N - acylamino acid amidohydrolase , EC 3.5.1.14 ) was investigated by using tannin immobilized on aminohexyl cellulose . - pubmed
The most active immobilized aminoacylase was obtained when aminoacylase was adsorbed to the immobilized tannin in a weak alkaline medium containing sodium chloride and n - butanol at 37 degrees C. The activity of the immobilized tannin - aminoacylase complex per unit volume was five times higher than that of the DEAE - Sephadex - aminoacylase complex used for industrial production of L - amino acids in our plants . - pubmed
In this work a steady - state kinetic analysis has been carried out on the catabolic ornithine carbamoyltransferase at pH 6.8 in the presence of the allosteric activator , phosphate . - pubmed
coli enzyme in the reverse direction , the initial velocity patterns converging on the abscissa were obtained with either citrulline or arsenate as variable substrate . - pubmed
Properties of mini - plasmids of the antibiotic resistance factor R1 carrying deleted and enlarged ampicillin transposons ( Tn3 ) . - pubmed
These results suggest that sigma receptors play a crucial role in the induction of methamphetamine - induced sensitization . - pubmed
Of more practical importance , the combined absence of CK , EMA , and vimentin in formalin - fixed , paraffin - embedded CCTL virtually precludes confusion with renal cell carcinoma . - pubmed
Uptake by ER - rich target tissue remained high when charcoal was used to prevent reabsorption of radiolabel from the digestive tract after its biliary excretion , and when the products of biliary excretion were removed by catheterization of the common bile duct . - pubmed
Sulfobromophthalein , bromophenol blue and rose bengal , which yielded an in vivo ' counter - transport ' phenomena , markedly inhibited ANS binding to cytosolic proteins . - pubmed
Sulfobromophthalein , which is one of the organic anions exhibiting the in vivo ' counter - transport ' phenomenon , remarkably inhibited ANS binding to ligandin fraction . - pubmed
The main representatives of substances with a vasodilatating action are the endothelial relaxation factor and prostacycline , as to vasoconstrictor substances it is endothelin , thromboxan A2 and some growth factors . - pubmed
Because of a history of prolonged infertility , conservative treatment ( including bed rest , antibiotics and intravaginal povidone - iodine pessaries ) was given . - pubmed
From comparison with structural data of other blue - copper proteins , it is concluded that the copper coordination environment is relatively rigid , which may be a clue to its high redox potential . - pubmed
Yeast tRNAPhe was incubated with the enzyme in the presence of double stranded RNA ( in this case polyI - polyC ) and ATP or deoxyATP . - pubmed
The reaction products were analyzed by ribonuclease T1 digestion of the tRNA , polyacrylamide gel electrophoresis and autoradiography . - pubmed
Using ATP , the 2 - 5A synthetase adds one , two or three AMP residues to the 3'-end of the tRNA whereas when dATP is replacing ATP , only one nucleotide unit is added . - pubmed
Within 5 minutes of intravenous injection of antigen , an acute , transient arthritis occurred in the test joints only , with swelling and extravasation of intravascular blue dye and 125I - labeled albumin , decreased numbers of stainable mast cells , and decreased histamine content of the joint synovium . - pubmed
Pretreatment of experimental animals with H1 and H2 antihistamines did not completely block the reaction . - pubmed
The PMNL - induced vascular smooth muscle relaxation was abolished by both hemoglobin and methylene blue and potentiated by both superoxide dismutase and captopril . - pubmed
Although suspension of PMNLs caused release of eicosanoids , thromboxane A2 and prostacyclin , from rings with intact endothelium , neither indomethacin nor the TxA2 - endoperoxide receptor antagonist SQ 29548 modified the effects of PMNLs on vascular smooth muscle tone . - pubmed
A sensitive and specific enzyme - linked immunosorbent assay ( ELISA ) for cholecystokinin octapeptide sulfate ( CCK-8S ) has been developed using N - terminal specific antibody for CCK-8S. - pubmed
The latter action suggests an effect on ovarian aromatase , on testicular 17 alpha / 17,20 - lyase activities and finally on cholesterol side - chain cleavage . - pubmed
The increase of progestins suggests that the 17 alpha / 17,20 - lyase activities are the most sensitive testicular enzymatic reactions to R 8110 . - pubmed
Unlike the neurite - promoting and neuronal - survival modulating agent laminin , it can not be depleted on poly ( L - alpha - ornithine )-coated plastic surfaces . - pubmed
NB IMR 32 cell extracts were electrophoresed using NaDodSO4 / PAGE and transferred to nitrocellulose . - pubmed
In contrast to the tolerance that is rapidly observed after repeated administration of LPS , MDP does not lose its capacity of inducing serum CSA after repeated injections . - pubmed
Repeated daily injections of MDP also fail to induce tolerance to the LPS - CSA inducing effect . - pubmed
This applies to glycosphingolipids ( GSLs ) and glycoproteins ( GPs ) . - pubmed
An important distinction between the two types of glycoconjugates seems to be that GPs , apart from carrying carbohydrates , have some biological function which is most likely associated with their protein moieties . - pubmed
These data suggest that the inhibitory action and potency of nucleoside dialdehydes on neuroblastoma growth are independent of their capacity to undergo a kinase - mediated phosphorylation at the 5 ' position . - pubmed
Human immunoglobulin M was reduced with concentrations of 2 - mercaptoethylamine chosen so that approximately 40 % of the immunoglobulin M was reduced to 7S subunits . - pubmed
Under these conditions , which selectively cleave intersubunit disulfides , J chain was released . - pubmed
The high - molecular - weight immunoglobulin M remaining after treatment with 2 - mercaptoethylamine had a sedimentation coefficient of 18.0 S and molecular weight of 1 million , and dissociated in 4 M guanidine into subunits similar in size to the 7S subunits . - pubmed
J chain was found in the 18.0S , noncovalently linked immunoglobulin M from which it was released only after more complete reduction with 10 mM dithiothreitol . - pubmed
The nature of chemically or photochemically reduced flavin of salicylate hydroxylase . - pubmed
The guinea - pig ileum was contracted much more slowly than by synthetic LT C4 or D4 and this contraction was not antagonized by FPL 55712 . - pubmed
In liver PE , PC , PS and PI it was respectively 4.3 , 4.9 , 3.8 and 0.4 , while in the analogous platelet lipids it was 3.0 , 4.0 , 0.9 and 0.6 . - pubmed
Feeding linolenate did not markedly elevate the levels of 22:5(n-3 ) or 22:6(n-3 ) in platelet PI , but the combined amounts of 22:5(n-3 ) and 22:6(n-3 ) in liver PI were 21.2 % , versus 2.9 % in chow - fed controls . - pubmed
Again , the 20:5(n-3)/20:4(n-6 ) ratio was lowest for platelet PI and PS and liver PI . - pubmed
These incorporation studies are consistent with the feeding studies which show that oleate is converted to 20:3(n-9 ) and incorporated into PI more readily than the analogous metabolism of 18:3(n-3 ) to 20:5(n-3 ) and its acylation into PI , which is an important source of unsaturated fatty acids for prostaglandin biosynthesis . - pubmed
A series of alkyldimethylbenzylammonium chlorides have been synthesized with n - alkyl chain lengths of C1 leads to C18 . - pubmed
The data fit to quadratic functions estimated for low ( C1 - C7 ) and high ( C8 - C16 ) alkyl chain length compounds was better than for a single quadratic describing the activity of the complete series ( C1 - C18 ) . - pubmed
These maximized at log P values corresponding to alkyl - chain lengths of approximately C7 and C14 respectively , and were suggestive of low and high affinity binding sites upon the cell surface . - pubmed
Evidence for essential lysines in heparin cofactor II . - pubmed
Covalent modification with pyridoxal 5 '- phosphate was used to study the function of lysyl residues in heparin cofactor II , a heparin - dependent plasma protease inhibitor . - pubmed
Reduction of the Schiff base with sodium borohydride resulted in modification of 3 - 4 lysyl residues of heparin cofactor II at high concentrations of pyridoxal 5 '- phosphate , one of which was protected in the presence of heparin . - pubmed
In contrast to native heparin cofactor II , the modified protease inhibitor did not bind to a heparin - agarose column . - pubmed
The results suggest that lysyl residues are essential for heparin cofactor activity during thrombin inhibition . - pubmed
[ Comparative study of the quantitative determination of kanamycin sulfate in ophthalmic films with a collagen base by microbiological and chemical methods ] . - pubmed
The results of the comparative study on microbiological and chemical quantitative determination of kanamycin sulfate in the ophthalmic films with the collagen base are presented . - pubmed
The intraocular films prepared with the use of 1 per cent collagen solution contain dexamethasone and kanamycin . - pubmed
Substitution of amino acids for casein was followed by a fall in urinary and plasma A1 . - pubmed
In the repair test , larval stock , consisting of meiotic recombination - deficient double mutant mei-9a mei-41D5 males and repair - proficient females , was exposed to the test agents , and the preferential killing of the mutant larvae was taken as evidence of the DNA - damaging effect . - pubmed
RNA and protein analyses indicated that the expression of p84NF - kappa B1 and p98NF - kappa B1 is restricted to certain tissues and that the phorbol myristate acetate - mediated induction of p84NF - kappa B1 and p105NF - kappa B1 differs in a cell - type - specific manner . - pubmed
The fdxA coding region is 231 nucleotides which codes for a 77 amino acids protein with the molecular weight of 8,744 except for iron - sulfur clusters . - pubmed
The 3D structure of a cyclosporin analogue in water is nearly identical to the cyclophilin - bound cyclosporin conformation . - pubmed
In these polar solvents the conformation is identical and very similar to the structure found in the cyclophilin - cyclosporin complex . - pubmed
The duration of the anovulatory period ( DAP , in d ) was estimated by bi - weekly blood progesterone measurements . - pubmed
Twenty - two of the SLE patients were receiving corticosteroids ( CS ) at the time of the study . - pubmed
The critical relative humidity ( CRH ) of the ranitidine HCl bulk powder was approximately 67 % relative humidity ( RH ) . - pubmed
Ranitidine HCl powder was unstable around the CRH . - pubmed
The lateral shift and increased intensity of GUS activity in the stem of rolC::uidA transformants during dormancy induction was shown to be associated with the accumulation of starch , and to be mimicked by incubating stem sections in sucrose , as well as glucose and fructose , but not sorbitol , prior to the GUS assay . - pubmed
Our results demonstrate that the activities of the rolC and 35S promoters varied in very different , unpredictable ways during the annual cycle of growth and dormancy in a perennial species , and indicate that the spatial and temporal variation in rolC promoter activity that we observed in the stem of transgenic hybrid aspen plants is attributable to cellular and seasonal changes in sucrose content . - pubmed
In this study , the effects of the deprivation of macrophage CSF ( M - CSF ) on enriched microglia in cultures were examined by cytochemical methods including in situ nick - end labeling for DNA fragmentation , and Carrazi 's hematoxylin nuclear staining . - pubmed
no substantial change in the percentage of students aware of the health risks of alcohol abuse from Q1 to Q6 was observed . - pubmed
Differential actions of haloperidol on neurotensin release might be due to regional differences in dopamine or sigma receptor subtypes associated with the neurotensin - containing neurons . - pubmed
Serum insulin , insulin C - peptide , and glucose were measured , fasting and 30 min after a 75 - g glucose load . - pubmed
The changes observed for fasting insulin , insulin C - peptide , glucose , serum lipids and the atherogenic index were also non - significant . - pubmed
The moths were trained to associate a conditioned stimulus ( CS ) , geraniol odour , with an unconditioned stimulus ( US ) , a sucrose solution . - pubmed
The aim of the present study was to further investigate the mechanism of the captopril - induced changes in early LV relaxation by comparing the effects of two sulphydryl and two non - sulphydryl containing ACE inhibitors in the same experimental preparation . - pubmed
The sulphydryl - containing ACE inhibitors captopril and zofenaprilat enhanced early LV relaxation , whereas the non - sulphydryl - containing ACE inhibitors lisinopril and quinaprilat did not . - pubmed
The effects of captopril and zofenaprilat were attenuated both by the nitric oxide - scavenger haemoglobin and the bradykinin B2 - kinin receptor antagonist HOE 140 . - pubmed
Neither the oxygen free - radical scavenger superoxide dismutase nor the sulphydryl - containing compound N - acetyl cysteine administered together with lisinopril had any effect on LV relaxation . - pubmed
These data demonstrate that inhibition of intra - cardiac ACE activity may acutely modulate LV relaxation through increased activity of the bradykinin - nitric oxide pathway . - pubmed
The presence of a sulphydryl group on the relevant ACE inhibitor appears to be essential for this LV relaxant effect . - pubmed
Glucose ( 25 mM ) induces an activation of the transcription of the fatty acid synthase gene , and this effect is markedly reduced by incubation of the cells with okadaic acid , an inhibitor of protein phosphatases 1 and 2A. - pubmed
A similar reduction in glucose - activated fatty acid synthase gene expression is obtained by incubation with 5 - amino - imidazolecarboxamide riboside , a cell - permeable activator of the AMP - activated protein kinase . - pubmed
Taken together , these results indicate that the glucose - induced expression of the fatty acid synthase gene involves a phosphorylation / dephosphorylation mechanism and suggest that the AMP - activated protein kinase plays an important role in this process . - pubmed
Characterization of antibacterial COOH - terminal proenkephalin - A - derived peptides ( PEAP ) in infectious fluids . - pubmed
Importance of enkelytin , the antibacterial PEAP209 - 237 secreted by stimulated chromaffin cells . - pubmed
Finally , we report the high phylogenetic conservation of the COOH - terminal PEAP , which implies some important biological function and we discuss the putative importance of enkelytin in the defensive processes . - pubmed
Should I be injecting insulin two or three times a day in an attempt to get very " tight " control of my blood sugar ? - pubmed
We looked at the expression of cyclin D1 in cortical neuronal cultures treated with either staurosporine to produce apoptotic death , or with glutamate , to produce a non - apoptotic death . - pubmed
Following the addition of either staurosporine or glutamate , cyclin D1 immunoreactivity did not change within 4 h. The cyclin D1 immunoreactivity was lost by 6 h with the appearance of either staurosporine - induced fragmented nuclei or glutamate - induced pyknotic nuclei . - pubmed
The formation of the main band was inhibited by EDTA , suggesting that this protease is a metalloprotease . - pubmed
METHODS : Dentin disks were treated with : ( 1 ) 35 % phosphoric acid ( PA ) 20 s ; ( 2 ) PA treatment followed by 1.5 % NaOCl , 2 min ; ( 3 ) PA treatment , followed by a 10 % NaOCl immersion for 120 h. SEM was used to analyze the morphology of dentin and its interface with dentin bonding agents ( DBAs ) , while shear bond strength tests were used to measure adhesion . - pubmed
All specimens were then fractured into two halves : One half was inspected under SEM ; the other half was sequentially placed in 10 % PA followed by 12.5 % NaOCl for 70 h , to remove all dentin from the resin replica of the original bonded interface . - pubmed
RESULTS : SEM observations showed that collagen fibrils were completely removed from the acid - etched surface by NaOCl treatment . - pubmed
In contrast , substantial declines in Cr concentrations were found in the house dust collected from homes located near the remediated waste sites : previously high - level homes had a median of 50 micrograms / g and mid - level homes had a median of 34 micrograms / g. Site remediation had a beneficial effect on household loadings of Cr , since no differences in post - remediation house dust Cr concentrations were found among the three groups . - pubmed
Immunological tests showed a normal total IgE but were negative in the CAP - FEIA test for penicilloyl G , penicilloyl V , amoxycilloyl and ampicilloyl . - pubmed
However , a strikingly high frequency of resistance to piperacillin or ceftazidime suggests that extended - spectrum beta - lactamase production in our Enterobacteriaceae may have been more prevalent than realized . - pubmed
In 14 subjects with leg DVT we examined the leukocyte filtration [ unfractionated , mononuclear cells ( MN ) , PMNs ] , PMN membrane fluidity and PMN cytosolic Ca2 + concentration . - pubmed
The observed J coupling constants were explained by the rigid sugar conformation model , and the sugar conformations were found to be between C3'-exo and C2'-endo with phi(m ) values of 26 degrees to 44 degrees , except for the second and 3 ' terminal residues C2 and C10 . - pubmed
The class of drugs known as the 5 - HT3 receptor antagonists have become widely used for chemotherapy - induced nausea and vomiting , and are considered a standard part of care for moderately- and highly - emetogenic chemotherapy in combination with corticosteroids . - pubmed
The sequence of full - length mDBR1 cDNA contained a single 515 amino acid open reading frame of 58 kDa protein . - pubmed
The increased hepatic corticosterone concentration due to the elevation of 11beta - HSD1 and high plasma corticosterone concentration may antagonize the action of insulin and cause insulin resistance . - pubmed
A molecular motif required for the activation of rat neutrophil phospholipase A ( 2 ) by organochlorine compounds . - pubmed
PLA ( 2 ) activation stimulated by each of the OC compounds was reduced by methyl arachidonyl fluorophosphonate , which inhibits both a cytosolic and a calcium - independent PLA ( 2 ) ( iPLA(2 ) ) , and by E-6 -( bromoethylene ) tetrahydro-3 -( 1 - naphthalenyl )- 2H - pyran-2 - one ( BEL ) , a selective inhibitor of iPLA ( 2 ) . - pubmed
For an amino acid sequence XYZ , we define two correction factors , Delta((X)Y)n , s and Delta(Y(Z))n , s , representing the effects on Y 's chemical shifts from the preceding residue ( X ) and the following residue ( Z ) , respectively , where X , Y , and Z are any of the 20 naturally occurring amino acids , n stands for ( 1 ) H ( N ) , ( 15 ) N , ( 1 ) H ( alpha ) , ( 13 ) C ( alpha ) , ( 13 ) C ( beta ) , and ( 13 ) C ' nuclei , and s represents the three secondary structural types beta - strand , random coil , and alpha - helix . - pubmed
Venous blood samples were drawn for 24 h for assay of total and unbound plasma ropivacaine , 3 - hydroxyropivacaine , pipecoloxylidide ( PPX ) , and serum alpha ( 1 )- acid glycoprotein ( AAG ) . - pubmed
To validate the expression profiling data , we evaluated the protein expression and promoter activity of prostate - specific antigen ( PSA ) , a gene induced by PC - SPES but repressed by DES . - pubmed
We provide evidence that androgen response elements ( ARE ) II and III within the promoter region are responsible for the suppressive effects of DES and stimulatory effects of PC - SPES . - pubmed
In addition , we show that the effects on PSA transcription are ARE specific in the case of DES while PC - SPES affects this promoter nonspecifically . - pubmed
Epsilon - amino caproic acid additive decreases fibrin bandage performance in a swine arterial bleeding model . - pubmed
The blood loss using the bandage with EACA ( TC - S+EACA ; 728.8+/-132 g , n=5 ) was much greater as compared to the TC - S bandage alone ( TC - S ; 237.8+/-47.9 g , n=6 , p=.02 ) . - pubmed
The time to " cessation of bleeding " ( T(b ) ) was also increased for animals treated with TC - S+EACA bandages compared to the TC - S controls ( 33.6+/-10.8 min vs. 9.2+/-2.2 min ; p=.05 ) . - pubmed
Although plasma fibrinogen concentration decreased in animals treated with the TC - S+EACA bandage , activated partial thromboplastin times ( aPTT ) and thrombin times ( TT ) were decreased . - pubmed
In conclusion , addition of EACA to the Nycomed product decreased fibrin bandage efficiency . - pubmed
Synthetic oligonucleotides ( ODNs ) are routinely analyzed using capillary gel electrophoresis ( CGE ) for size - sieving based separations as well as electrospray mass spectrometry ( ESI - MS ) for identification . - pubmed
Effects of CGE buffer concentration , capillary length , separation and orifice voltage on CGE separation and MS detection of a phosphodiester ODN mixture were examined . - pubmed
The inward current was blocked by the presence of dihydrokainate ( 0.5 mM ) , shown by others to readily block the GLT1 isoform . - pubmed
Reactivation of phosphorylated acetocholinesterase by pyridine-2 - aldoxime methiodide . - pubmed
TARTRATE - INHIBITED ACID PHOSPHATASE IN BENIGN PROSTATIC HYPERTROPHY AND IN PROSTATIC CANCER . - pubmed
We have investigated the relationship between the induction of AICD by phorbol 12 - myristate 13 - acetate plus ionomycin during the cell cycle and the expression of survivin , an inhibitor of the apoptosis protein ( LAP ) family . - pubmed
Isoprenylcysteine carboxyl methyltransferase modulates endothelial monolayer permeability : involvement of RhoA carboxyl methylation . - pubmed
The role of RhoA and Rac1 carboxyl methylation in modulating endothelial monolayer permeability is not known . - pubmed
In this study , we found that inhibition of isoprenylcysteine - O - carboxyl methyltransferase ( ICMT ) with adenosine plus homocysteine or N - acetyl - S - geranylgeranyl - l - cysteine decreased RhoA carboxyl methylation , RhoA activity , and endothelial monolayer permeability , suggesting that RhoA carboxyl methylation may play a role in the ICMT - modulated monolayer permeability . - pubmed
Similar studies showed no effect of ICMT inhibition on Rac1 carboxyl methylation or localization . - pubmed
PAECs stably overexpressing ICMT demonstrated increased RhoA carboxyl methylation , membrane - bound RhoA , and RhoA activity . - pubmed
These effects were blunted by adenosine plus homocysteine and by inhibition of RhoA , but not by inhibition of Rac1 . - pubmed
These results indicate that ICMT modulates endothelial monolayer permeability by altering RhoA carboxyl methylation and activation , thus changing the organization of intercellular junctions . - pubmed
Therefore , carboxyl methylation of RhoA may modulate endothelial barrier function . - pubmed
The structure - activity relationships ( SAR ) for affinity and intrinsic efficacy of analogues of the full agonist N6 - cyclopentyladenosine ( CPA ) are emphasized . - pubmed
The SAR of affinity and intrinsic efficacy of the 2 ' , 3 ' , and especially the 5 '- deoxy derivatives of CPA is presented . - pubmed
From this analysis , the nature of the interactions of specific atoms and substituents of the CPA molecule with the A ( 1 )- adenosine receptor are deduced and presented pictorially . - pubmed
At 48 hours after reperfusion , AST ( P = .03 ) and LDH ( P = .05 ) levels were significantly lower in the gadolinium group . - pubmed
Inhibition of nucleoside transport proteins by C8 - alkylamine - substituted purines . - pubmed
4 - nitrobenzylthioinosine ( NBTI , 1 ) is a well - known inhibitor for the nucleoside transport protein ENT1 . - pubmed
The vitamin K - dependent gamma - carboxylation system in the endoplasmic reticulum membrane responsible for gamma - carboxyglutamic acid modification of vitamin K - dependent proteins includes gamma - carboxylase and vitamin K 2,3 - epoxide reductase ( VKOR ) . - pubmed
An understanding of the mechanism by which this system works at the molecular level has been hampered by the difficulty of identifying VKOR involved in warfarin sensitive reduction of vitamin K 2,3 - epoxide to reduced vitamin K ( 1 ) H ( 2 ) , the gamma - carboxylase cofactor . - pubmed
Identification and cloning of VKORC1 , a proposed subunit of a larger VKOR enzyme complex , have provided opportunities for new experimental approaches aimed at understanding the vitamin K - dependent gamma - carboxylation system . - pubmed
However increased activity of the gamma - carboxylation system , where VKOR provides the reduced vitamin K ( 1 ) H ( 2 ) cofactor , was measured only in cells transfected with VKORC1 and the double transfected cells . - pubmed
The results show that VKOR is the rate - limiting step in the gamma - carboxylation system and demonstrate successful engineering of cells containing a recombinant vitamin K - dependent gamma - carboxylation system with enhanced capacity for gamma - carboxyglutamic acid modification . - pubmed
Upregulation of RPL6 was associated with enhanced resistance to multiple anticancer drugs ( adriamycin , vincristine , etoposide , 5 - fluorouracil and cisplatin ) and to adriamycin - induced apoptosis . - pubmed
Downregulation of RPL6 reversed MDR and sensitized cells to adriamycin - induced apoptosis . - pubmed
Alteration of RPL6 showed no obvious influence on intracellular adriamycin accumulation , glutathione content and expression of glutathione S - transferase . - pubmed
A series of combretastatins substituted with epoxides , amides and small alkyl groups has been synthesised and evaluated for cytotoxicity and their ability to inhibit the assembly of tubulin . - pubmed
Beyond its antioxidative properties , PON1 possesses additional antiatherogenic properties against macrophage foam cell formation : attenuation of cholesterol and oxidized lipids influx , inhibition of macrophage cholesterol biosynthesis and stimulation of macrophage cholesterol efflux . - pubmed
Nutritional antioxidants , such as polyphenols , increase PON1 mRNA expression and activity , by an aryl hydrocarbon receptor - dependent mechanism . - pubmed
Some drugs and nutrients including dietary antioxidants and polyphenols considerably increase the activities of paraoxonases which , in turn , can reduce oxidative stress and atherosclerosis development . - pubmed
Since the cloning of the intracellular steroid receptors / transcription factors , it has been assumed that most if not all of the actions of the gonadal steroids are mediated via these intracellular receptors . - pubmed
In addition , gonadal steroids can affect second messenger systems , including calcium and various kinases to prompt and/or alter cell signaling . - pubmed
After co - administration of estradiol and progesterone , progesterone reversed estradiol 's analgesic effect in Phase II , however , estradiol did not reverse progesterone 's analgesic activity in Phase I. - pubmed
To determine if estradiol effects are receptor - mediated , tamoxifen ( selective estrogen receptor mediator , 15 mg / kg ) or alpha - estradiol ( an inactive isomer of estradiol , 20 microg ) were utilized . - pubmed
Tamoxifen decreased the number of formalin - induced flinches during Phase II while alpha - estradiol did not affect any formalin - induced responses . - pubmed
AIM : The aim of this analysis was to examine the average rate and variation of alcohol - related injury across emergency department ( ED ) studies , the effect of usual drinking on likelihood of alcohol - related injury , whether cross - study variation in rate of alcohol - related injury can be explained by between - study difference in usual consumption and whether social - cultural contextual variables help explain cross - study variations , after between - study difference in usual consumption has been controlled . - pubmed
Two receptors for ADP , the G ( q )- protein - coupled P2Y1 and G ( i )- protein - coupled P2Y12 and one receptor for ATP , the P2X1 ion channel , have been identified on platelets . - pubmed
RESULTS : Compared with control group , the expression of TERT was increased in 5 micromol / kg Cd group and 10 micromol / kg Cd group , c - Myc protein was increased in 10 micromol / kg Cd group , and the expression of p53 protein was increased in 5 micromol / kg group and 10 micromol / kg Cd group . - pubmed
TERT expression in Se + 10 micromol / kg Cd group was lower than that of 10 micromol / kg Cd group significantly . - pubmed
c - Myc protein was decreased in Se + 10 micromol / kg Cd group compared with 10 micromol / kg Cd group . - pubmed
p53 protein of Se + 5 micromol / kg Cd group and Se + 10 micromol / kg Cd group were decreased significantly compared with 5 micromol / kg Cd group and 10 micromol / kg Cd group respectively . - pubmed
Indole , a core nucleus for potent inhibitors of tubulin polymerization . - pubmed
Several tubulin polymerization inhibitors characterized by the presence of an indole nucleus have been obtained from natural sources or have been prepared by semi - synthesis . - pubmed
We have reviewed anti - tubulin agents obtained by synthesis having an indole as core nucleus . - pubmed
A high frequency of the kdr resistance gene has not prevented pyrethroid - treated nets from functioning , but it is important to develop alternative fabric treatments in case stronger forms of resistance emerge . - pubmed
We concluded that in all normal subjects their breath test was negative after 3 minutes , and at least a 5 minute wait post Salamol inhalation is necessary before roadside breath testing should be undertaken , otherwise a failed breath test is likely . - pubmed
In conclusion , the MASP1 / 3 gene harbours a low - frequent polymorphic site resulting in an amino acid substitution , which may influence the function of the gene product . - pubmed
Neuronal Gq / 11 - coupled dopamine receptors : an uncharted role for dopamine . - pubmed
There is strong evidence for the existence of Gq / 11 - coupled dopamine receptors in the brain but the mechanism by which dopamine signaling activates Gq / 11 , or its roles in neuronal function , are only just beginning to be understood . - pubmed
During apoptosis induction by sanguinarine and chelerythrine , reactive oxygen species ( ROS ) was rapidly generated and DeltaPsi(mt ) dissipated , while Bax , Bcl-2 and Bcl - X (( L / S ) ) proteins ' content in the mitochondrial fraction did not change significantly . - pubmed
Caspase-3 activation and DNA fragmentation were considerably inhibited by N - acetyl - cysteine ( NAC ) . - pubmed
Chelidonine induced only a slight release of cytochrome c ( 12h ) , parallel to caspase-3 activation . - pubmed
Subtilisin - A was colyophilized with various types of support materials , such as Amberlite IRC-50 , Celite545 , chitosan , DEAE - cellulose , DOWEX-1 , zeolite , glass bead , and polystyrene . - pubmed
The colyophilized enzyme was used for the optical resolution of racemic 1 - phenylethylamine with 2,2,2 - trifluoroethylbutyrate in 3 - methyl-3 - pentanol . - pubmed
The genes of penile development ( HOX , FGF , Shh ) and testicular determination ( WT1 , SRY ) and those regulating the synthesis [ luteinizing hormone ( LH ) receptor ] and action of androgen ( 5alpha reductase , androgen receptor ) can cause hypospadias if altered . - pubmed
The cells with impairment of LDL uptake produced DCP . - pubmed
Fat soluble vitamins are taken up into cells through clathrin - mediated endocytosis , in which the dynamic polymerization of F - actin plays a crucial role . - pubmed
METHODS : Key studies supporting the importance of surrogate markers as diagnostic and prognostic predictors of cardiovascular and renal clinical outcomes in hypertension , as well as what is known about the effects of renin - angiotensin - aldosterone system - blocking agents on these biomarkers were reviewed . - pubmed
RESULTS : Clinical data supporting the use of surrogate markers for heart failure , such as brain natriuretic peptide ( BNP ) and N - terminal prohormone BNP ; markers for renal function , such as urinary albumin to creatinine ratio ( UACR ) , urinary albumin excretion rates ( UAER ) , and creatinine , reflecting glomerular filtration ; and markers of cardiac remodeling , such as left ventricular hypertrophy and calculations of left ventricular mass index ( LVMI ) , were reviewed for their utility in improving prognosis and treatment efficacy . - pubmed
CL(int ) values for a cocktail incubation of phenacetin , diclofenac , diazepam , bufuralol , midazolam , and hydroxycoumarin were 4.9 + /- 3.4 , 18 + /- 7.2 , 5.1 + /- 4.9 , 6.3 + /- 3.3 , 9.8 + /- 5.8 and 22 + /- 14 microl min(-1)/10(6 ) cells , respectively , and they correlated well with corresponding CL(int ) values using cryopreserved hepatocytes from 25 different donors . - pubmed
Influence of Pb ( II ) ions on the EPR properties of the semiquinone radicals of humic acids and model compounds : high field EPR and relativistic DFT studies . - pubmed
X - band ( 9.76 GHz ) and high field ( 416.00 GHz ) electron paramagnetic resonance spectroscopy ( EPR ) was used to study the interactions between Pb ( II ) ions and semiquinone radicals of natural humic acids and their simple models . - pubmed
Principal component analysis ( PCA ) and positive matrix factorization ( PMF ) analysis were followed by multilinear regression analyses to identify and quantify PAH source contributions , together with spatial and temporal trends . - pubmed
The PMF coke oven emission factor showed the greatest variability in total loadings , consistent with the large PAH emissions from the steel industries and the large influence of wind direction on PAH concentrations . - pubmed
The PMF coke oven impact factor showed good correlations with two commonly used PAH diagnostic ratios when the ratios were combined into a single ratio . - pubmed
The immunogen was prepared by linking MEL hapten with carrier protein via carbodiimide method . - pubmed
The MAbs showed high specificity with low cross - reactivity ( & lt ; or = 1 % ) toward cyanurate , ammelide , and ammeline . - pubmed
PURPOSE : 68Ga - DOTA - DPhe1,Tyr3 - octreotate ( 68Ga - DOTATATE ) is a somatostatin analogue that shows high affinity for somatostatin receptor subtype 2 ( sst2 ) and has been used for imaging neuroendocrine tumours . - pubmed
RESULTS : 68Ga - DOTATATE uptake was noted in the sst2 - expressing organs such as pituitary , thyroid , stomach wall , spleen , adrenals , kidneys , pancreas and prostate and in the liver and salivary glands , with excreted activity in the bowel and in the pelvicalyceal system of the kidneys and urinary bladder . - pubmed
We show that the rpoS allele of Ty19 ( rpoS(Ty19 ) ) led to the synthesis of a sigma ( S )( Ty19 ) protein carrying a single glycine - to - valine substitution at position 282 in sigma ( S ) domain 4 , which was much more dependent than the wild - type sigma ( S ) protein on activation by Crl , a chaperone - like protein that increases the affinity of sigma ( S ) for the RNA polymerase core enzyme ( E ) . - pubmed
In addition to acetylsalicylic acid , a cyclooxygenase inhibitor , ADP - receptor blockers are frequently used for anti - platelet therapy . - pubmed
Moreover , dexamethasone , lipopolysaccharides ( LPS ) and other relevant adipokines such as leptin and adiponectin were able to modulate chemerin , LCN2 and SAA3 mRNA expression alone and when coadministered . - pubmed
Current evidence supports such a role for the ABI1 protein phosphatase and , by inference , protein kinase elements in gating K (+ ) channel sensitivity to pH(i ) and ABA . - pubmed
We describe a robust method for detecting SHH , WNT , and non - SHH / WNT molecular subgroups in formalin - fixed medulloblastoma samples . - pubmed
BACKGROUND : The diagnostic accuracy of flat panel digital detector ( FPDD ) cinefluoroscopy for coronary artery calcium ( CAC ) detection , compared to multiple detector computed tomography ( MDCT ) , is not known . - pubmed
Median values of urine IL-8 / creatinine levels were 61.5 , 4.64 , 15.5 , 14.3 , 1.06 and 4.19 pg / mumol in groups A , B , C , D , E and F respectively . - pubmed
Taking the backbone of tenofovir and lamivudine / emtricitabine as a reference , we found that , with the exception of regimens including zidovudine , the effect of other nucleoside reverse transcriptase inhibitor backbones had little effect on SVR . - pubmed
In this study , a commercially available silica - coated paper was explored in an attempt to improve the analysis of therapeutic drugs in dried blood spots ( DBS ) . - pubmed
RESULTS : The method used for estimating glomerular filtration rate was serum creatinine in 36.6 % , creatinine clearance in 41.5 % and equations in 22 % ; none reported using cystatin - C. - pubmed
In conclusion , we have demonstrated that a beta - cell specific disruption of the miRNAs network , although allowing for apparently normal beta - cell development , leads to progressive impairment of insulin secretion , glucose homeostasis and diabetes development . - pubmed
We now report that isoflurane anesthesia in young , pre - symptomatic Tau . - pubmed
The prescription drug memantine , identified as an NMDA receptor antagonist , prevented the post - anesthesia upper airways dysfunction and alleviated tauopathy in the nucleus ambiguus and Kolliker - Fuse . - pubmed
Successful resolution of inflammation and increased regulatory T cells in sirolimus - treated post - transplant allograft hepatitis . - pubmed
Pharmacological mechanisms of 5 - HT3 and tachykinin NK1 receptor antagonism to prevent chemotherapy - induced nausea and vomiting . - pubmed
However , in 2003 , a new 5 - HT ( 3 ) receptor antagonist was introduced into the market ; unlike first generation 5 - HT ( 3 ) receptor antagonists , palonosetron was found to be effective in preventing both acute and delayed chemotherapy induced nausea and vomiting . - pubmed
Recent mechanistic studies have shown that palonosetron , in contrast to first generation receptor antagonists , exhibits allosteric binding to the 5 - HT ( 3 ) receptor , positive cooperativity , persistent inhibition of receptor function after the drug is removed and triggers 5 - HT ( 3 ) receptor internalization . - pubmed
Further , in vitro and in vivo experiments have shown that palonosetron can inhibit substance P - mediated responses , presumably through its unique interactions with the 5 - HT ( 3 ) receptor . - pubmed
ADAM-8 has an overall fold similar to those of other ADAM members , including a central five - stranded beta - sheet and a catalytic Zn ( 2 + ) ion . - pubmed
This effect with high BMI might hypothetically be a result of reduced inhibition of aromatase and suppression of plasma estrogen levels and might be overcome by the use of an increased dose of anastrozole or , alternatively , the use of a more potent AI such as letrozole . - pubmed
Maximum degradation was observed in undoped and ZnO - doped beta - TCP samples , which degraded to 41 % and 68 % of the original strength before soaking in SBF . - pubmed
Neuropeptide CART ( cocaine - amphetamine - regulated transcript ) , a hypothalamus and midbrain enriched neurotransmitter with an antioxidant property , can be found in mitochondria , which is the main source of reactive oxygen species . - pubmed
In this article , we summarize recent progress in studies investigating the relationship between CART , dopamine , and the pathophysiology of PD , with a focus on mitochondria - related topics . - pubmed
Poly ( 3 - hexylthiophene ) ( P3HT ) monolayer has been investigated by scanning tunneling microscopy ( STM ) . - pubmed
The monolayer was dominated by three kinds of ordered structure ( I , II , and III ) , where the thiophene main chains lied parallel to one another , but high resolution STM images revealed that the arrangement of the hexyl side chains was different . - pubmed
In structure II , the interchain distance was significantly larger at ~1.52 nm , and the hexyl side chains were liquid - like . - pubmed
Structure III exhibited similar interchain distance as structure II , but the hexyl side chains were perpendicular to the main chain and were interdigitated . - pubmed
In addition to these ordered domains , individual poly ( 3 - hexylthiophene ) chains in various special configurations were observed , and their unfolding into more stable structures was tracked by dynamic STM , which provides evidence that P3HT is a relatively flexible polymer . - pubmed
Altered ketones suggest that systemic metabolism may play a critical role in preventing neurological injury , while altered succinate provides a possible explanation for hypoxia - inducible factor 1 - alpha ( HIF-1alpha ) stabilization in HI injury . - pubmed
We applied photoluminescence , PL excitation and absorbance spectra to determine the influence of crystal symmetry , surface to volume ratio and ligands on the optical properties of doped Eu ( 3 + ) ions . - pubmed
Unilateral intrastriatal administration of QA ( 300 nmol/4 microl ) significantly reduced body weight , impaired behavior ( locomotor activity , beam balance and memory performance ) , caused oxidative damage ( increased lipid peroxidation , nitrite concentration , depleted super oxide dismutase and reduced glutathione ) , demonstrated mitochondrial dysfunction ( decreased Complex - I , II , III , and IV activities ) , increased striatal lesion volume and altered the levels of TNF - alpha , caspase-3 as well as BDNF expression , as compared to sham group . - pubmed
aureus ( hVISA ) using the glycopeptide resistance detection e - test and population analysis , giving a prevalence rate of 2.19 % . - pubmed
Pre - administration of SB334867 , an orexin-1 receptor antagonist , attenuated the degree of inhibition of swallowing frequency induced by the administration of orexin - A. - pubmed
Pre - injection of SB334867 into the medial DVC disrupted the inhibitory response induced by fourth ventricular administration of orexin - A. - pubmed
I prostanoid receptor - mediated inflammatory pathway promotes hepatic gluconeogenesis through activation of PKA and inhibition of AKT . - pubmed
In this study , we observed dysregulated expression of cyclooxygenase-2 , prostacyclin biosynthesis , and the I prostanoid receptor ( IP ) in the liver 's response to diabetic stresses . - pubmed
IP deletion depressed cAMP - dependent CREB phosphorylation and elevated AKT phosphorylation by suppressing PI3K - gamma / PKC - zeta - mediated TRB3 expression , which subsequently downregulated the gluconeogenic genes for glucose-6 - phosphatase ( G6Pase ) and phosphoenol pyruvate carboxykinase 1 in hepatocytes . - pubmed
We exogenously administrated one of three different compounds to adults , that is , ( a ) cortisol , ( b ) glucocorticoid receptor antagonist mifepristone RU486 or ( c ) sham ( saline ) , and observed their cleaning behaviour during the following 45min . - pubmed
We also found that the cytochrome P450 CYP6ER1 was significantly overexpressed in 12 imidacloprid - resistant populations tested when compared with a laboratory susceptible strain , with fold changes ranging from ten- to 90 - fold . - pubmed
In contrast , another cytochrome P450 CYP6AY1 , also implicated in imidacloprid resistance , was underexpressed in ten of the populations and only significantly overexpressed ( 3.5 - fold ) in a single population from India compared with the same susceptible strain . - pubmed
Further selection of two of the imidacloprid - resistant field strains correlated with an approximate threefold increase in expression of CYP6ER1 . - pubmed
In macrophages the alpha7 nicotinic acetylcholine receptor ( alpha7nAChR ) modulates production of inflammatory cytokines , cholesterol accumulation and lipoprotein uptake . - pubmed
Mechanisms through which omega-3 FA act are manifolds and still a matter of investigation : beside their interaction with ion channel and their influence on plasma membrane fluidity , probably the main effect is acting as competitor for cytochrome P-450 ( CYP ) with respect to omega-6 FA . - pubmed
The aim of the present study is to rationalize acrylamide pendant Phos - Tag in - gel discrimination of phosphorylated and non - phosphorylated plant protein species with standard immunoblot analysis , and optimize sample preparation , efficient electrophoretic separation and transfer . - pubmed
Mucus - buffers - phospholipid layer as pre - epithelial barrier , enhanced by prostaglandins and epidermal growth factor , remains a vanguard of mucosal protection . - pubmed
The surface epithelial cells , elaborating mucins , buffers , phospholipids , prostaglandins , trefoil peptides , peptide growth factor and their receptors , heat shock proteins , cathelicidins , and beta - defensins form the second line of defense . - pubmed
This microcirculation is instrumental for supply of oxygen , nitric oxide , hydrogen sulfide and removal of ad hoc generated toxic substances as well as for continuous mucosal cell renewal from progenitor cells , secured by growth factors accompanied by survivin preventing early apoptosis . - pubmed
In addition , pUL27 is necessary for the full antiviral activity of the pUL97 kinase inhibitor maribavir ( MBV ) . - pubmed
Is nucleotide exchange sufficient to activate K - Ras4B ? - pubmed
With the use of NMR and molecular dynamics ( MD ) simulations , we observed that in solution , farnesylated guanosine 5 '- diphosphate ( GDP)-bound K - Ras4B is predominantly autoinhibited by its hypervariable region ( HVR ) , whereas the GTP - bound state favors an activated , HVR - released state . - pubmed
In the autoinhibited state , the HVR is sandwiched between the effector lobe and the membrane ; in the active state , with membrane - anchored farnesyl and unrestrained HVR , the catalytic domain fluctuates reinlessly , exposing its effector - binding site . - pubmed
Notably , we also observe HVR - autoinhibited K - Ras4B - GTP states , with GDP - bound - like orientations of the helices . - pubmed
Thus , we propose that the GDP / GTP exchange may not be sufficient for activation ; instead , our results suggest that the GDP / GTP exchange , HVR sequestration , farnesyl insertion , and orientation / localization of the catalytic domain at the membrane conjointly determine the active or inactive state of K - Ras4B. - pubmed
Importantly , K - Ras4B - GTP can exist in active and inactive states ; on its own , GTP binding may not compel K - Ras4B activation.-Jang , H. , Banerjee , A. , Chavan , T. S , Lu , S. , Zhang , J. , Gaponenko , V. , Nussinov , R. The higher level of complexity of K - Ras4B activation at the membrane . - pubmed
The total bilirubin , direct bilirubin and tumor markers ( CA-199 , CA-242 , CEA ) before and after surgery , the biliary stent patency status was assessed , and the survival time was evaluated . - pubmed
With binding to specific nicotinic acetylcholine receptors , nicotine can induce migration and proliferation of vascular cells , and hence enhances angiogenesis . - pubmed
KEY WORDS : Angiogenesis ; Atherosclerosis ; nicotinic acetylcholine receptors ( nAChRs ) ; Nicotine . - pubmed
Evolution of Src Homology 2 ( SH2 ) Domain to Recognize Sulfotyrosine . - pubmed
Protein tyrosine O - sulfation is considered as the most common type of post - translational tyrosine modification in nature and plays important roles in extracellular biomolecular interactions . - pubmed
To facilitate the mapping , biological study , and medicinal application of this type of post - translational modification , we seek to evolve a small protein scaffold that recognizes sulfotyrosine with high affinity . - pubmed
We focused our efforts on the engineering of the Src Homology 2 ( SH2 ) domain , which represents the largest class of known phosphotyrosine - recognition domain in nature and has a highly evolvable binding pocket . - pubmed
The best mutant , SH2 - 60.1 , displayed more than 1700 fold higher sulfotyrosine - binding affinity than that of the wild - type SH2 domain . - pubmed
Sex hormone - binding globulin regulation of androgen bioactivity in vivo : validation of the free hormone hypothesis . - pubmed
Sex hormone - binding globulin ( SHBG ) is the high - affinity binding protein for androgens and estrogens . - pubmed
According to the free hormone hypothesis , SHBG modulates the bioactivity of sex steroids by limiting their diffusion into target tissues . - pubmed
SHBG increased total androgen and estrogen concentrations via hypothalamic - pituitary feedback regulation and prolonged ligand half - life . - pubmed
Daratumumab ( DARA ) , a monoclonal antibody ( MoAb ) , was developed to treat relapsed / refractory multiple myeloma ( RRMM ) . - pubmed
In chromosome aberration ( CA ) and micronucleus ( MN ) tests , roxithromycin did not show genotoxic effect . - pubmed
ROS levels were partially reduced by incubation with PP2 ( c - Src inhibitor ) or IL1RN , and further reduced by using the NOX1 / 4 inhibitor GKT137831 . - pubmed
Placentas were collected and subjected to histological evaluation , the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene pleckstrin homology - like domain , family A , member 2 ( PHLDA2 ) , as well as chromatin immunoprecipitation ( ChIP ) for histone marks within the promoter of PHLDA2 . - pubmed
ChIP revealed an increase in the permissive mark dimethylation of lysine 4 on histone H3 ( H3K4me2 ) , surprisingly associated with the silent allele of PHLDA2 , and a decrease in the inhibitory mark H3K9me2 in NT samples . - pubmed
PNC-27 is a peptide synthesized of amino acids of the p53 - MDM-2 binding domain . - pubmed
METHODS : The impact of exposure to paclitaxel on the surface expression of MDM-2 was assessed with the use of flow cytometry . - pubmed
The cytotoxic effect of PNC-27 was dependent on its binding to MDM-2 . - pubmed
These patients had a reduced transfer coefficient for carbon monoxide ( Kco ; 33 % [ interquartile range , 30%-35 % ] predicted ) and younger age at diagnosis ( 29 years ; interquartile range , 23 - 38 years ) and more interlobular septal thickening and mediastinal lymphadenopathy on computed tomography of the chest compared with patients with PAH without EIF2AK4 mutations . - pubmed
The transport of bile acids facilitated by NTCP is an important factor in establishing bile flow . - pubmed
GCDC and 3S - GLC but not CDCA were transported by NTCP . - pubmed
The efficient uptake of GCDC , TCA and 3S - GLC by NTCP enabled the determination of kinetics . - pubmed
The apparent CLint value for GCDC was 20 - fold greater ( 153 + -53mul / mg protein / min ) than the apparent CLint for TCA ( 6.92 + -4.72mul / mg protein / min ) and apparent CLint for 3S - GLC ( 8.05 + -1.33mul / mg protein / min ) . - pubmed
These kinetic results provide important complementary data on the substrate selectivity and specificity of NTCP to transport bile acids . - pubmed
NTCP transports GCDC with greater efficiency than TCA and has the same efficacy for 3S - GLC and TCA . - pubmed
Computed tomography ( CT ) , F - fluorodeoxyglucose ( F - FDG ) , positron emission tomography - computed tomography ( PET - CT ) , and transthoracic echocardiography showed a space - occupying lesion 7 cm x 5 cm x 5 cm in the right atrium . - pubmed
Inhibition of PAK in LA by microinjection of the PAK inhibitor IPA-3 30 minutes before fear conditioning enhanced long - term but not short - term fear memory formation . - pubmed
No significant correlations were found between plasma AOPP concentration or ImAnOx and PTH , or their changes in time . - pubmed
( 2 ) This benefit may be related rather to the direct action of cinacalcet than to the serum PTH concentration decrease . - pubmed
MANF improves the MPP+/MPTP - induced Parkinson ' s disease via improvement of mitochondrial function and inhibition of oxidative stress . - pubmed
OBJECTIVE : This study aimed to investigate the therapeutic effect of mesencephalic astrocyte - derived neurotrophic factor ( MANF ) on the MPTP / MPP+-induced model of Parkinson ' s disease ( PD ) and the potential mechanism . - pubmed
Two weeks later , Rotarod test , immunohistochemistry , and detection of dopamine ( DA ) and its metabolites , superoxide dismutase ( SOD ) , glutathione ( GSH ) , and malondialdehyde ( MDA ) were performed . - pubmed
MANF pre - treatment significantly decreased the MPP +-induced reduction of cell viability ( P<0.01 ) , inhibited the ratio of Bax / Bcl-2 expression ( P<0.01 ) , activated gene expression levels of HMOX1 ( P<0.01 ) and SOD2 ( P<0.05 ) , and reversed MPP +-induced loss of mitochondrial membrane potential ( P<0.01 ) . - pubmed
Contrary to MOG , DNA - histone and DNA - met - BSA only stimulated the formation of anti - DNA antibodies hydrolysing DNA with a long delay ( 15 - 20 days after immunization ) . - pubmed
In this study , we designed a folate - grafted PEI600 - CyD ( H1 ) nanoparticle - mediated DNA vaccine containing an adjuvant of absent in melanoma 2 ( AIM2 ) and a tumor - specific antigen of carbonic anhydrase IX ( CAIX ) for renal carcinoma therapy . - pubmed
RESULTS : The GST - NUDT9 - H fusion protein was stabilized with lysis buffer containing 0.5 % n - dodecyl - beta - d - maltoside ( DDM ) , and wash buffer containing 0.025 % DDM in size - exclusion chromatography system , and finally the NUDT9 - H with high purity was obtained after cleaved by thrombin ( 1 U/2 mg fusion protein ) for 24 h. CONCLUSIONS : Due to the poor stability of NUDT9 - H , it is necessary to add DDM in extraction and purification buffer to stabilize the conformation of NUDT9 - H , so as to increase its yields and purity . - pubmed
In this review , we will discuss pathways targeted in new topical treatments such as antagonist of proteinase - activated receptor-2 , the endocannabinoid system , neurotrophins and tropomyosin - related kinase A receptor , the transient receptor potential - vanilloid or transient receptor potential - melastatine ion channels . - pubmed
The skin irritation testing indicated that CB2 and CF2 were safe after application and did not cause any skin irritation . - pubmed
While CB2 showed insignificant effects , therefore , it was demonstrated that CF2 decreased skin erythema content and increased skin elasticity and hydration significantly but had an insignificant effect on skin melanin content with respect to time . - pubmed
OBJECTIVE : To assess the safety and efficacy of crisaborole 2 % ointment - a nonsteroidal , phosphodiesterase-4 inhibitor - in the treatment of intertriginous , anogenital , and facial psoriasis . - pubmed
Development of an sEMG sensor composed of two - layered conductive silicone with different carbon concentrations . - pubmed
To achieve robust sEMG measurements in an EMG prosthetic system , this study proposes a surface electromyogram ( sEMG ) sensor with a novel electrode structure composed of two - layered conductive silicone with different carbon concentrations . - pubmed
We hypothesized there is an optimal carbon concentration for achieving a large sEMG amplitude with robustness to external perturbation , and we empirically determined this optimal concentration . - pubmed
Our results clearly demonstrate an optimal carbon concentration for robust sEMG measurements , and suggest that the robust measurement of sEMG is supported by the capacitance component of the sensor system . - pubmed
Migrating neurons within the cortical plate of E18.5 Lmnb1 - deficient embryos exhibited NM ruptures , evident by the escape of the nuclear - localized reporter into the cytoplasm and NM discontinuities by electron microscopy . - pubmed
To test whether mechanical forces on the cell nucleus are relevant to NM ruptures in migrating neurons , we examined cultured Lmnb1 -/- neurons when exposed to external constrictive forces ( migration into a field of tightly spaced silicon pillars ) . - pubmed
Graft phagocytic cells were depleted in vivo at different time points during G - CSF / rapamycin treatment , to identify their role in Tr1 - cell induction and consequently in graft survival . - pubmed
The role of percentage saturation of hemoglobin with oxygen ( oxygen saturation ) , blood pressure and early use of point - of - care ultrasound in differential diagnosis and specific management is detailed . - pubmed
Prenatal androgen ( PNA ) exposure mimics reproductive dysfunction of the common fertility disorder polycystic ovary syndrome ( PCOS ) and reduces prepubertal GnRH neuron activity . - pubmed
Significance Statement Gonadotropin - releasing hormone ( GnRH ) neuron activity and associated GnRH release link the neuronal control of reproduction to peripheral secretion of reproductive hormones . - pubmed
Here , we reveal that prepubertal suppression of GnRH neuron activity without androgen exposure leads to similar increases in adult GnRH neuron activity , and a mild degradation in reproductive cycles . - pubmed
This provides a clearer understanding of the role prepubertal GnRH neuron activity plays in establishing adult reproductive function and suggests additional androgen - dependent programming actions are required for complete reproductive disruption in this model and perhaps PCOS . - pubmed
All isolates were multidrug resistant but vancomycin susceptible ; they carried the icaA gene but tested negative for the pvl and ses genes . - pubmed
Here we set out to characterize the expression status of LINC00426 in osteosarcoma and understand its mechanistic involvement in incidence of doxorubicin ( Dox ) resistance . - pubmed
The direct association between LINC00426 and miR-4319 was analyzed by pulldown assay with biotin - labelled probes . - pubmed
RESULTS : LINC00426 was significantly up - regulated in Dox - resistant osteosarcoma ( OS ) both in vitro and in vivo , which intimately associated with unfavorable prognosis . - pubmed
SiRNA - mediated knockdown of LINC00426 remarkably compromised cell viability and proliferation in Dox - resistant OS cells , which accompanied with decrease of IC50 and activation of caspase-3 . - pubmed
CONCLUSION : Ectopic introduction of miR-4319 significantly surmounted the Dox resistance in OS cells , while miR-4319 inhibition in LINC00426 - deficient cells greatly restore this phenotype . - pubmed
We uncovered the important contribution of LINC00426 / miR-4319 to Dox resistance in osteosarcoma . - pubmed
We test hypothesis whether high methionine diet ( HMD , an amino acid high in red - meat ) a substrate for Hcy can cause skeletal muscle and growth retardation and treatment with probiotics ( PB ) mitigate muscle dysfunction . - pubmed
A greenhouse experiment as a completely randomized design with six treatments of bio / organic - fertilizers ( no bio - fertilizer ( NF ) , RHB , RHC , MR , RHB+MR , and RHC+MR ) and three levels of SDZ application ( 0 , 100 , and 200 mg kg-1 ) was performed for 7months with three replicates . - pubmed
The results revealed that the application of RHC , RHB , and MR had a significant impact on the reduction of the toxicity effects of SDZ on plant properties . - pubmed
The same results were obtained with macro ( NPK ) and micro ( Fe , Zn , Cu , and Mn ) nutrients uptake by the shoot in which the lowest values of nutrients uptake were observed in treatment of 200 mg kg-1 of SDZ with no bio - fertilizers . - pubmed
And the characterization of BcHep - I after purified with Ni - NTA affinity chromatography suggested that it is a mesophilic enzyme . - pubmed
BcHep - I can be activated by 1 mM Ca2 + , Mg2 + , and Mn2 + , while severely inhibited by Zn2 + , Co2 + , and EDTA . - pubmed
These aspects have motivated the search for collectors that exhibit superior performance without the health , safety and environmental ( HSE ) concerns associated with xanthates . - pubmed
3 -( 2,4,6 - trinitrophenylhydrazone -( methyl ) rifamycin SV is a strong inhibitor for all the polymerases tested and belongs to the C class inhibitors of reverse transcriptase . - pubmed
From protective action of the template - primer and other data it might be suggested that the rifamycin derivatives act at an early step(s ) in DNA synthesis catalyzed by reverse transcriptase . - pubmed
Trypsin was then covalently bonded onto the CMPS membrane by diazotization . - pubmed
The activity of immobilized trypsin reaches up to 10200 U / g ; 15 mg trypsin was immobilized on 1 g CMPS membrane . - pubmed
The two forms of stromelysin were subsequently separated , again using Procion Red - Sepharose . - pubmed
The acridine half - mustard ICR-170 - induced reversion of the mutant am15 , which has a single base - pair deletion , at a frequency of between 9 and 28 X 10(-6 ) . - pubmed
The purified MAb was found to be over 95 % pure based on the results from SDS - PAGE . - pubmed
The role of the activated c - H - ras-1 locus in the initiation of mammary neoplasia was analysed by studying seven transplantable , DMBA - induced Balb / c premalignant mammary hyperplasias . - pubmed
Modulation by protein kinase C but not by cyclic nucleotides . - pubmed
Protein kinase C activators attenuated GnRH - induced phosphoinositide hydrolysis , while neither cyclic AMP analogs nor cyclic GMP analogs were effective . - pubmed
These results indicate that phosphoinositide hydrolysis represents an important postreceptor transducing mechanism for GnRH action at the gonadotroph and that protein kinase C ( but not cyclic nucleotides ) may exert a negative feedback control on GnRH receptor - coupling mechanisms . - pubmed
Linkage and segregation analyses of apolipoproteins A1 and B , and lipoprotein cholesterol levels in a large pedigree with excess coronary heart disease : the Bogalusa Heart Study . - pubmed
Robust methods were employed , using data from a single large pedigree , to screen serum apolipoprotein A1 and B levels , serum lipoprotein cholesterol levels , and ratios of serum lipoprotein cholesterol fractions to apolipoprotein A1 and B levels for genetic linkage to 31 polymorphic markers . - pubmed
Segregation analyses were performed for each of the apolipoprotein and lipoprotein cholesterol fractions to obtain estimates for use in applying likelihood methods of linkage analysis . - pubmed
Results from the segregation analyses were consistent with major gene determination of apo B and HDL - C levels , the HDL - C to apo A1 ratio , the LDL - C to apo B ratio , and a measure of relative content of cholesterol in HDL - C and LDL - C. Linkage between haptoglobin and the HDL - C / apo A1 ratio was suggested , with a lod score of 1.72 at theta = 0.05 . - pubmed
Indoxyl sulfate could not be removed effectively by conventional hemodialysis because of its strong binding to serum albumin . - pubmed
Equilibrium dialysis demonstrated that indoxyl sulfate inhibited the binding of salicylate to albumin , and that 3 - carboxy-4 - methyl-5 - propyl-2 - furanpropionic acid inhibited the binding of indoxyl sulfate to albumin . - pubmed
The review provides a conclusive evidence which suggests that chromium , especially its hexavalent form , is both toxic and carcinogenic , but its trivalent form is physiologically essential in the metabolism of insulin . - pubmed
Mutations at the chlA or chlE locus , which are required for molybdenum cofactor biosynthesis , similarly do not affect the activity of the heat - treated extract in the in vitro activation process . - pubmed
None of the other known pleiotropic chlorate resistance loci ( chlD , chlG ) are required for the expression of its activity . - pubmed
We conclude that a low - molecular - weight substance , distinct from the active molybdenum cofactor , is required to bestow activity on the molybdoenzyme nitrate reductase during its biosynthesis . - pubmed
Tryptic degradation of the 50 K fragment abolished , or reduced greatly , the binding of heat - treated subfragment 1 to actin in solution but not on nitrocellulose overlays . - pubmed
It is likely that during its differentiation the primary mesenchyme provides some of its own extracellular microenvironment in the form of collagen and proteoglycans . - pubmed
Injected neurons were first examined in whole slices , and subsequently , in sectioned material , characterized immunocytochemically using antisera to vasopressin- and oxytocin - associated neurophysins ( VP - NP and OT - NP respectively ) . - pubmed
Six of the single cells and all the dye coupled pairs were immunoreactive with VP - NP antiserum and not reactive with OT - NP antiserum . - pubmed
[ Dentin liquid : determination of creatinine and lactate dehydrogenase content ] . - pubmed
Antagonistic effects of 25 ( OH ) vitamin D3 and peptide hormones on the activation of adenylate cyclase / cyclic AMP system in renal tissue in vitro . - pubmed
[ Mechanism of the anti - aggregation action of thyroxine and insulin ] . - pubmed
Thyroxine and insulin inhibit the aggregation capacity of platelets . - pubmed
Based on the data obtained a hypothesis is advanced according to which thyroxine inhibits the platelet aggregation , interfering with the release of arachidonic acid from phospholipids of platelets ( thrombocytopathy A ) , whereas insulin -- by blocking the cyclooxygenase activity ( thrombocytopathy B ) . - pubmed
Analyses of the tubulin by two - dimensional polyacrylamide gel electrophoresis , isoelectric focusing , and peptide mapping indicated that the beta - tubulin complex remained constant regardless of source but that some heterogeneity was present in the alpha subunit . - pubmed
After 120 minutes of incubation , however , Tris inhibited the conversion of proPTH to parathormone ( PTH ) , judged by a decrease in cellular and secreted radioactive PTH and a corresponding increase in radioactive cellular proPTH . - pubmed
With both concentrations of A23187 and the low concentration of X537A there were no changes in amounts of radioactive proPTH , moderate decreases in cellular and secreted radioactive PTH , and little discernible distension of the Golgi membranes . - pubmed
At 17 microM X537A there was moderate increase in amount of radioactive proPTH , a marked decrease in amount of radioactive PTH and swelling of the Golgi membranes . - pubmed
Taken together , these findings suggest that Tris inhibited conversion of proPTH to PTH by disrupting the Golgi zone - the site of conversion of proPTH to PTH ; that A23187 and the low concentration of X537A decreased production of PTH by enhancing its degradation ; and that X537A at the higher concentration acted both by inhibiting conversion of proPTH to PTH and by enhancing the degradation of PTH . - pubmed
The effects of chronic morphine administration were tested in a paradigm of 7 days saline , 7 days morphine , 1 day morphine+naloxone , and 6 days post - drug saline . - pubmed
[ The renin - aldosterone profile in hypertension ] . - pubmed
Moderate positive correlation was observed between the level of aldosterone and renin activity ; aldosterone - renin index at the stage of the disease remains unchanged . - pubmed
MM - MTX were prepared by the following methods : ( A ) reaction of MTX with poly ( ethylene glycol ) 1500 ( PEG ) to form a poly ( ethylene glycol )- methotrexate conjugate ( PEGMTX ) which was then added to a ferrous / ferric ion salt solution to give MM - MTX I ; ( B ) reaction of ferrous / ferric ion salts with PEG to give a ferromagnetic polymer complex which was then coupled with MTX to give MM - MTX II ; ( C ) MM - MTX IIIA were prepared by reacting MTX with amino - terminated magnetic microspheres , commercially available , in the presence of 1 - ethyl-3,3 - bis ( methylamino ) propylcarbodiimide ( EDCI ) ; ( D ) reaction of aminohexanol with di - tert - butyl dicarbonate to form an [ N -( tert - butoxycarbonyl ) amino ] hexanol ( t - Boc - AH ) , which was then coupled with MTX in the presence of 1,3 - dicyclohexylcarbodiimide and 4 - pyrrolidinopyridine to give a t - Boc - AH - MTX conjugate , which was then saturated with hydrogen chloride to give an aminohexanol - methotrexate ( AH - MTX ) conjugate . - pubmed
Drug content of the magnetic microsphere - methotrexate conjugates as determined by HPLC was 0.45 % ( w / w ) , 4.0 % ( w / w ) , and 6.3 % ( w / w ) MTX for MM - MTX I , MM - MTX II , and MM - MTX IIIB , respectively. - pubmed
Aryl hydrocarbons ( AHs ) such as 2,3,7,8 - tetrachlorodibenzo - p - dioxin and benzo [ a ] pyrene activate the sequence - specific DNA - binding activity of the AH receptor . - pubmed
Methylamine protection experiments and immunofluorescence studies suggested that CNF2 enters the cytosol of the target cell through an acidic compartment and induces the reorganization of actin into stress fibers . - pubmed
The [ 32P]ADP - ribosylated Rho proteins from CNF2 - treated cells migrated slightly more slowly in SDS / PAGE than did the labeled proteins from the control cells . - pubmed
PROCEDURE : Colonic smooth muscle strips were suspended in physiologic buffer solution , attached to isometric force transducers , and contracted with acetylcholine ( ACh ; 10(-9 ) to 10(-4)M ) , substance P ( SP ; 10(-10 ) to 10(-6)M ) , cholecystokinin ( CCK ; 10(-11 ) to 10(-8)M ) , potassium chloride ( KCl ; 10 to 80 mM ) , or electrical field stimulation ( EFS ; 25 V , 1 to 30 Hz , 0.5 - millisecond duration ) . - pubmed
RESULTS : Passive isometric stress was not altered , but the active isometric stress responses of megacolon smooth muscle to ACh , SP , CCK , KCl , and EFS were significantly ( P & lt ; 0.05 ) diminished , compared with healthy controls . - pubmed
The contractile response of megacolon smooth muscle to EFS , and the inhibition of this response by tetrodotoxin , suggest that myenteric and submucosal plexus neurons in megacolon smooth muscle are functional . - pubmed
The diminished isometric stress responses to receptor occupancy ( ACh , SP , and CCK ) and membrane depolarization ( KCl ) further suggest that the disorder involves disturbance in the activation of smooth muscle myofilaments . - pubmed
After 23 d of treatment , concentrations of ACTH and cortisol were measured during 7.5 h. On d 24 , cortisol concentrations were determined at time -.5 , 0 , .5 , 1 , 2 , 3 , 4 , and 5 h after i.v . - pubmed
Cortisol concentrations did not differ among treatment groups in the baseline series and after ACTH administration . - pubmed
[ Normal cerebral perfusion of 99mTc - ECD brain SPECT -- evaluation by an anatomical standardization technique ] . - pubmed
Therefore , the specific purpose of this study was to investigate the normal distribution pattern of 99mTc - ECD SPECT image . - pubmed
The primary structure of barley grain serpin BSZ7 was deduced from a cDNA encoding 397 amino - acid residues . - pubmed
Functional integrity of hepatocytes was evaluated from lactate dehydrogenase ( LD ) leakage , urea synthesis in the perfusion medium and lipid peroxides ( LP ) formation . - pubmed
Low concentration of sodium nitroprusside ( as NO donor ) significantly decreased LD leakage , increased the rate of urea synthesis and increased trypan blue exclusion by hepatocytes in anoxia / reoxygenation or cumene hydroperoxide injured ( 0.2 mM ) cells . - pubmed
Using these methods , we investigated the effects of the sulphated octapeptide of cholecystokinin ( CCK8 ) on gastric emptying and on stomach motility in vivo and in vitro . - pubmed
The decrease in amplitude was not affected by the nitric oxide synthase inhibitor NG - nitro - l - arginine methyl ester hydrochloride ( L - NAME ; 10(-4 ) mol l-1 ) . - pubmed
Depending on the concentration and experimental arrangement , CCK8 had either inhibitory or excitatory effects on the cardiac stomach , suggesting the possible presence of different types of CCK receptor . - pubmed
Increasing plasma neopterin and persistent plasma endothelin during follow - up after acute cerebral ischemia . - pubmed
Plasma neopterin was higher at follow - up ( 6.3 nmol / L [ 3.7 - 21.6 ] vs 5.6 nmol / L [ 3.5 - 17.2 ] ; p & lt ; 0.05 ) than at the acute stage , whereas the plasma ET-1 concentration was unchanged . - pubmed
Plasma concentrations of both neopterin and ET-1 correlated directly with age both in the acute stage ( r = 0.42 and r = 0.35 , respectively ; p & lt ; 0.01 ) and after follow - up ( r = 0.34 ; p & lt ; 0.05 and r = 0.27 ; p = 0.05 , respectively ) . - pubmed
Linomide downregulates autoimmunity through induction of TH2 cytokine production by lymphocytes . - pubmed
In this study , in an effort to elucidate the mechanisms by which Linomide suppresses EAE , and autoimmunity in general , we investigated the in vivo effects of this drug on the TH1 / TH2 lymphocyte balance , which is important for the induction or inhibition of autoireactivity . - pubmed
A significant upregulation of IL-4 , IL-10 and TGFbeta was observed following treatment with Linomide , which peaked at day 10 ( IL-10 ) or day 15 ( IL-4 ) . - pubmed
We speculate that Ihyp - act may have a role in basal Cl- absorption , acting as a Cl- sensor to maintain optimal volume and composition of airway surface liquid . - pubmed
The new technique - acid coinjection - achieves a similar retention enhancement for arginine with only a minimal effect on the rest of the separation . - pubmed
The Al ( 3 +)-activated current exhibited higher permeability to malate(2- ) than to Cl (- ) ( P ( mal )/ P ( Cl ) & gt ; or = 2.6 ) and was inhibited by anion channel antagonists , niflumate and diphenylamine-2 - carboxylic acid . - pubmed
Antidiuretic action of vasopressin : quantitative aspects and interaction between V1a and V2 receptor - mediated effects . - pubmed
( 1 ) Vasopressin ( VP ) , or antidiuretic hormone , is secreted in response to either increases in plasma osmolality ( very sensitive stimulus ) or to decreases in plasma volume ( less sensitive stimulus ) . - pubmed
( i ) Increase in water permeability along the entire CD ( via AQP2 ) . - pubmed
( 3 ) Because urine is first diluted along the nephron before being concentrated in the medulla , VP is required , not only for urine concentration , but first for re - equilibration of tubular fluid osmolality with plasma osmolality , a step taking place in the renal cortex , and achieved through the reabsorption of large quantities of water ( more than what is subsequently reabsorbed in the medulla to concentrate urine ) . - pubmed
Surface expression of P - selectin was minimal in unstimulated HBMEC ; however , it was significantly augmented upon stimulation with histamine ( 10(-7)-10(-3 ) M ) and thrombin ( 0.01 - 1 U / ml ) . - pubmed
Histamine - induced surface expression of P - selectin was blocked by the histamine H2 receptor antagonist cimetidine . - pubmed
The H1 receptor antagonist mepyramine had no effect . - pubmed
Expression was reduced by the extracellular calcium chelator EDTA and blocked by the cyclic AMP phosphodiesterase inhibitor rolipram . - pubmed
Thus histamine and thrombin both increase P - selectin expression in HBMEC . - pubmed
Histamine mediates expression through the H2 , but not the H1 , receptor and calcium , whereas expression is reduced by cyclic AMP . - pubmed
RESULTS : Five mutations in the beta - globin gene which are common in the Indian population were detected in 92.3 % of mutant chromosomes , whereas 3.1 % of chromosomes carried rare mutations followed by 0.8 % haemoglobin E and 0.4 % sickle cell mutations . - pubmed
In order to evaluate the ability of multivalent glycosides based on a beta - cyclodextrin core as site - specific molecular carriers , a study on both the inclusion complexation behaviour and lectin binding affinity of branched and hyperbranched beta - cyclodextrins is presented . - pubmed
The persubstitution of the primary face of the beta - cyclodextrin with saccharides led to a slight increase of the binding constant values for the inclusion complexation with ANS relative to the native beta - cyclodextrin . - pubmed
METHODS : RBC extracts were trimethylsilylated ( TMS ) and analyzed by gas chromatography / mass spectrometry ( GC / MS ) . - pubmed
In the whole sample , multiple regression analysis of dose - normalized concentration revealed significant effects of time postdose ( -18 % per 12 hours delay , P & lt ; 0.05 ) , age & gt;/=60 years ( +27 % , P & lt ; 0.005 ) , cigarette smoking ( -12 % , P & lt ; 0.05 ) , and comedication with fluvoxamine ( +74 % , P & lt ; 0.001 ) , paroxetine , fluoxetine , or sertraline ( considered together , +32 % , P & lt ; 0.05 ) , venlafaxine ( +27 % , P & lt ; 0.05 ) , and inducers of P450 enzymes ( -40 % , P & lt ; 0.001 ) . - pubmed
We investigated differentiation of CD4 T cells responding to Ag presented by bone marrow - derived dendritic cells ( DC ) in association with MHC class II ( MHC II ) molecules . - pubmed
MHC II - peptide - specific T cells responding to this Ag were polarized to a Th1 cytokine profile in a CD40- , CD28- , MyD88- , and IL-12 - dependent manner . - pubmed
DC that took up the same FcR - targeted liposomes and then were exposed to methyl - beta - cyclodextrin , which chelates cholesterol and dissociates lipid microdomains , also stimulated Th2 differentiation . - pubmed
They caused a severe decrease of the respiratory function and altered the assembly of the iron - sulfur protein in the bc ( 1 ) complex , as observed by immunodetection . - pubmed
Suppressor mutations that partially restored the respiratory function impaired by S152P or G291D were found in or close to the hinge region of the iron - sulfur protein , suggesting that this region may play a role in the stable binding of the subunit to the bc ( 1 ) complex . - pubmed
Y279C caused a significant decrease of the bc ( 1 ) function and perturbed the quinol binding . - pubmed
The ankyrin repeat is one of the most frequently observed amino acid motifs in protein databases . - pubmed
More significantly , our data demonstrate that in vivo treatment with the petroleum ether extract exerts an insulin - sensitizing action by enhancing the activity of the two major intracellular signal transduction pathways of the hormone 's receptor . - pubmed
Heparin was infused at a rate of 16 - 32 IU/ kg / h to maintain an ACT of 160 - 180 seconds . - pubmed
We have previously reported that YO-2 , a selective plasmin inhibitor , induces thymocyte apoptosis . - pubmed
To elucidate the mechanism of YO-2 - induced apoptosis , other YO compounds with different plasmin inhibitory action were tested for the pro - apoptotic activity in this study . - pubmed
Lactams as EP4 prostanoid receptor agonists . - pubmed
The latter lactams were devised using a pharmacophore for EP ( 4 ) receptor activity . - pubmed
gamma - Lactams were characterized for their prostanoid EP receptor affinities and EP(4 ) activity and found to be selective for the EP ( 2 ) and EP ( 4 ) receptors or selective for the EP ( 4 ) subtype . - pubmed
Women with PCOS and the MBS had significantly higher levels of serum free testosterone ( P = 0.002 ) and lower levels of serum SHBG ( P = 0.001 ) than women with PCOS without the MBS . - pubmed
In this analysis of the charge distribution of 7 proteins , we estimate the solvation free energy contribution to the total free energy by exploring the 2(zeta ) possible ionization states of the whole molecule , with zeta being the number of ionizable groups in the amino acid sequence , for every conformation in the ensembles of 7 proteins . - pubmed
CONCLUSION : These results suggest that exercise training induces the altered expression in several genes including prostaglandin , CNP , and nitric oxide in the aorta and that these molecular changes ( particularly eNOS as its protein expression was altered ) may contribute , at least in part , to the beneficial effect of exercise training on aortic stiffness . - pubmed
Both cell lines were refractory to Fas ; whereas , alpha - TEA sensitized them to Fas signaling . - pubmed
alpha - TEA increased levels of Fas message , protein and membrane - associated Fas . - pubmed
Neutralizing antibodies to Fas or Fas L partially blocked alpha - TEA - induced apoptosis . - pubmed
alpha - TEA induced prolonged activation of c - Jun N - terminal kinase ( JNK ) and its substrate c - Jun ; Bax conformational change ; and cleavage of Bid and caspases-8 , - 9 and - 3 . - pubmed
Chemical inhibitors of JNK , and caspases blocked alpha - TEA - induced apoptosis . - pubmed
alpha - TEA decreased phosphorylation of protein kinase B ( Akt / PKB ) and extracellular signal - regulated kinase ( ERK1 / 2 ) , as well as cellular FLICE - like inhibitory protein ( c - FLIP ) and Survivin protein levels . - pubmed
Knockdown of Akt and ERK activity using phosphoinositide - 3 - kinase ( PI3 K ) and mitogen - activated protein kinase kinase ( MKK1 ) inhibitors enhanced alpha - TEA - induced apoptosis . - pubmed
Over - expression of constitutively active Akt2 and MKK1 blocked alpha - TEA - induced apoptosis . - pubmed
Considering the importance of nitric oxide generation in the regulation of vessel tone , reduced endothelial nitric oxide synthase ( eNOS ) expression in alveolar macrophages exposed to short - term silica ( Si ) suggests the possibility of Si - induced changes in endothelial functions . - pubmed
Si - induced reduced NO association with elevated endothelium - derived hyperpolarizing factor ( EDHF ) in response to ACh , together with reduced NO sensitization , might have clinical importance in cardiovascular pathology . - pubmed
In summary , brain VIP and PACAP both appear to exert generally catabolic effects on energy metabolism in the chick , but their influence on body temperature and glucose metabolism differs and their central effects do not appear to be mediated by the same receptors . - pubmed
Studies were conducted on day 20 , when blood was collected for corticosterone measurement as an indication of hypothalamus - pituitary - adrenal ( HPA ) axis activity , and colonic function was studied in tissues mounted in Ussing chambers . - pubmed
Pulmonary neuroendocrine cells ( PNEC ) , including neuroepithelial bodies ( NEB ) , are amine- and peptide ( for example , bombesin)-producing cells that function as hypoxia / hypercapnia - sensitive chemoreceptors that could be involved in the pathophysiology of sudden infant death syndrome ( SIDS ) . - pubmed
Before differentiation , significantly more cells incorporated BrdU in cultures exposed to FGF2 ( 19 - fold ; P & lt ; 0.001 ) and FGF8 ( 3 - fold ; P & lt ; 0.05 ) compared to controls . - pubmed
After differentiation , biochemical analyses showed significantly more dopamine and FA1 in conditioned medium from both FGF2 and FGF8 expanded cultures than in controls . - pubmed
Glucoamylase is an industrially extremely important enzyme in the fermentative production of ethanol , used in the enzymatic conversion of starch into high glucose and fructose syrups . - pubmed
Induction by 0.75 % methanol for 72 h led to synthesis of secreted glucoamylase . - pubmed
Based on the activity profiles and the availability of materials , five inhibitors were examined for their ability to compete with [ ( 125 ) I ] iodoarylazidoprazosin labeling of ABCG2 , increase binding of the anti - ABCG2 antibody 5D3 , and prevent P - glycoprotein or multidrug resistance protein 1 - mediated transport . - pubmed
None of the compounds affected P - glycoprotein - mediated rhodamine 123 transport , whereas three affected multidrug resistance protein-1 - mediated calcein transport at 25 mumol / L , suggesting that the compounds are relatively specific for ABCG2 . - pubmed
Isoprostanes are prostaglandin - like bioactive compounds that are biosynthesized in vivo independent of cyclooxygenases , principally through free - radical catalyzation of arachidonic acid . - pubmed
Effects of urea on protein stability have been studied from all - atom molecular dynamics simulations of ubiquitin , G311 protein , and immunoglobulin binding domain ( B1 ) of streptococcal protein G ( GB1 ) in water and 8 M aqueous urea solution . - pubmed
The mechanism of the change in the solvent environment and the early events in protein unfolding by urea have been identified with emphasis on the change in the interactions of hydrophilic and hydrophobic parts of the protein by calculating the potential of mean force ( PMF ) . - pubmed
Urea replaces the protein - protein and protein - water contacts by forming stronger contacts with the protein , which is indicated by the longer survival times of the protein - urea hydrogen bonds . - pubmed
Crystalline structure of ceria particles controlled by the oxygen partial pressure and STI CMP performances . - pubmed
The effect of the crystalline structures of nano - sized ceria particles on shallow trench isolation ( STI ) chemical mechanical planarization ( CMP ) performance was investigated . - pubmed
The crystalline structures of ceria particles were analyzed by the high - resolution TEM nano - beam diffraction pattern . - pubmed
In the STI CMP evaluation , the ceria slurry prepared with the cubic CeO ( 2 ) shows enhanced performances of the oxide - to - nitride removal selectivity . - pubmed
The severity of cholestasis and hepatic injury was determined by changes in the plasma enzyme activities of aspartate aminotransferase , alanine aminotransferase , gama glutamin transferase , and levels of total bilirubin and direct bilirubin . - pubmed
Malondialdehyde , nitric oxide , and superoxide dismutase were determined to evaluate the oxidative status in the liver tissue . - pubmed
Myeloperoxidase activity and levels of tissue hydroxyproline were determined to assess neutrophil activation and collagen accumulation , respectively . - pubmed
Sphingosylphosphorylcholine also inhibited the increase in liver malondialdehyde ; nitric oxide levels significantly and also attenuated the depletion of superoxide dismutase in the liver after BDL . - pubmed
Similarly , the increase in tissue myeloperoxidase activity and hydroxyproline owing to BDL was also attenuated by the SPC treatment . - pubmed
Although one antagonist -- maraviroc , a CCR5 inhibitor to treat AIDS -- has been registered as an approved drug , this is the only success so far . - pubmed
This paper presents a GC - MS analytical procedure for determining proteinaceous materials , glycerolipids , natural waxes and terpenoid resins in the same paint micro - sample . - pubmed
The proteinaceous fraction is analysed by GC - MS after hydrolysis and derivatisation of the freed amino acids . - pubmed
Drugs with a strong rationale that have failed or have not shown univocal results in clinical trials so far include eicosapentaenoic acid , cannabinoids , bortezomib , and anti - TNF - alpha MoAb . - pubmed
Several emerging drugs have shown promising results but are still under clinical investigation ( thalidomide , selective cox-2 inhibitors , ghrelin mimetics , insulin , oxandrolone , and olanzapine ) . - pubmed
During subunit assembly , this segment of the chaperone is displaced by an amino - terminal extension of an incoming subunit in a reaction termed donor - strand exchange . - pubmed
Here we show that the binding of complexes between FimC and the different pilus subunits to the amino - terminal substrate recognition domain of FimD is an extremely fast process , with association rate constants in the range of 10(7)-10(8 ) M(-)(1 ) s(-1 ) at 20 degrees C. Furthermore , we demonstrate that the ordered assembly of pilus subunits is a consequence of the usher 's ability to selectively catalyze the assembly of defined subunit - subunit pairs that are adjacent in the mature pilus . - pubmed
Water -- more than just a green solvent : a stereoselective one - pot access to all - chiral tetrahydronaphthalenes in aqueous media . - pubmed
Using rationally designed substrates , the reaction conditions have been optimized and the one - pot process has been applied to a series of nitroolefin acrylates and aldehydes . - pubmed
Here we summarize evidence that the acute actions of the steroid arise from actin signaling cascades centrally involved in long - term potentiation ( LTP ) . - pubmed
E2 's actions on transmission and plasticity were completely blocked by latrunculin , a toxin that prevents actin polymerization . - pubmed
Estrogen activated the small GTPase RhoA , but not the related GTPase Rac , and phosphorylated ( inactivated ) synaptic cofilin , an actin severing protein targeted by RhoA. - pubmed
Collectively , these results indicate that E2 engages a RhoA & gt;ROCK&gt ; cofilin&gt ; actin pathway also used by brain - derived neurotrophic factor and adenosine , and therefore belongs to a family of ' synaptic modulators ' that regulate plasticity . - pubmed
A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP . - pubmed
Three cases ( 20.0 % ) were refractory to corticosteroid plus diverse immunosuppressive therapy and were treated with anti - TNF agents , all of them with disease remission . - pubmed
The LPO treatment downregulated levels of reactive oxygen species in osteoclasts . - pubmed
Ultimately , LPO treatment led to decreased expression of c - Fos and NFAT2 . - pubmed
A rapid turnover of phosphate pools in the leaves is essential for energy transfer and metabolism within photosynthesis , and consequently , we hypothesized that PHR1 is needed for adaptation to high - light stress during P deficiency . - pubmed
Compared to the wild - type and the overexpressor line , the phr1 mutant has decreased levels of phosphate , anthocyanins and carbohydrates during combined P deficiency and light stress . - pubmed
We conclude that PHR1 is needed for the metabolic balance , for retaining P(i ) levels and for inducing anthocyanin production , and during P deficiency PHR1 is vital for adaptations to avoid permanent damage to photosystems during high - light conditions . - pubmed
Endoplasmic reticulum stress decreases intracellular thyroid hormone activation via an eIF2a - mediated decrease in type 2 deiodinase synthesis . - pubmed
To test whether D2 is normally processed through ER - associated degradation , ER stress was induced in cells that endogenously express D2 by exposure to thapsigargin or tunicamycin . - pubmed
ER stress - induced loss of D2 was prevented in the absence of T(4 ) , by blocking the proteasome with MG-132 or by treatment with chemical chaperones . - pubmed
Notably , ER stress did not alter D2 activity half - life but rather decreased D2 synthesis as assessed by induction of D2 mRNA and by [ ( 35 ) S ] methionine labeling . - pubmed
FITC - labeled GW - H1 was found to attach to cell membrane initially , then translocated into the cytoplasm , and eventually membranous organelles or nucleus . - pubmed
INTRODUCTION : Ticagrelor , an oral P2Y ( 12 ) receptor antagonist used as part of dual antiplatelet therapy in the treatment of acute coronary syndromes ( ACS ) , has many favorable characteristics when compared with the more frequently used P2Y ( 12 ) receptor antagonist clopidogrel . - pubmed
Also , the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein , reducing sugar , and nitrogen levels were monitored during the stationary cultivation . - pubmed
The highest Lac activity was obtained as 2247.62 + - 75 U / L on day 10 in the presence of 750 microM Mn2 + , while the highest MnP activity was attained as 2198.44 + - 65 U / L on day 15 in the presence of 500 microM Mn2 + . - pubmed
Kinetic analysis of iron - dependent histone demethylases : alpha - ketoglutarate substrate inhibition and potential relevance to the regulation of histone demethylation in cancer cells . - pubmed
The Jumonji C domain - containing histone demethylases ( JmjC - HDMs ) are alpha - ketoglutarate ( alphaKG)-dependent , O ( 2 )-activating , non - heme iron enzymes that play an important role in epigenetics . - pubmed
A continuous O ( 2 ) consumption assay reveals that HDMs have low affinities for O ( 2 ) , suggesting that these enzymes can act as oxygen sensors in vivo . - pubmed
An interesting case of alphaKG substrate inhibition was found , and the kinetic data suggest that alphaKG inhibits JMJD2C competitively with respect to O ( 2 ) . - pubmed
JMJD2C displays an optimal activity in vitro at alphaKG concentrations similar to those found in cancer cells , with implications for the regulation of histone demethylation activity in cancer versus normal cells . - pubmed
Our aim was to determine the effects of dexamethasone treatment on wild - type CFTR expression . - pubmed
Within 24 h , dexamethasone ( 10 nM ) showed a 0.3 - fold decrease in CFTR mRNA expression , and a 3.2 - fold increase in alphaENaC mRNA expression compared with control groups . - pubmed
Dexamethasone ( 10 nM ) induced a 1.97 - fold increase in the total protein of wild - type CFTR , confirmed by inhibition by mifepristone . - pubmed
To access surface protein expression , biotinylation followed by Western blotting showed that dexamethasone treatment led to a 2.35 - fold increase in the amount of CFTR in the cell surface compared with the untreated control groups . - pubmed
Once protein translation was inhibited with cycloheximide , dexamethasone could not increase the amount of CFTR protein . - pubmed
Dexamethasone did not alter the degradation of wild - type CFTR . - pubmed
Assessment of the B band of CFTR within 15 min of metabolic pulse labeling showed a 1.5 - fold increase in CFTR protein after treatment with dexamethasone for 24 h. Chaperone 90 ( HSP90 ) binding to CFTR increased 1.55 - fold after treatment with dexamethasone for 24 h , whereas chaperone 70 ( HSP70 ) binding decreased 0.30 fold in an immunoprecipitation assay . - pubmed
CONCLUSION : Mature wild - type CFTR protein is regulated by dexamethasone post transcription , involving cotranslational mechanisms with HSP90 and HSP70 , which enhances maturation and expression of wild - type CFTR . - pubmed
During late larval stages , neural proliferation becomes insensitive to dietary nutrients , and the steroid hormone ecdysone acts on Delta / Notch signaling to promote the switch from symmetric mitoses to asymmetric neurogenic divisions . - pubmed
Here , we study the role of oxysterols and their nuclear receptors , Liver X Receptor ( LXR ) , in myelinating glial cells of the central and peripheral nervous systems . - pubmed
We show that the transcripts of Wnt components ( Disheveled , TCF3 , beta - catenin ) are strongly repressed by oxysterols in Schwann cells but are activated in oligodendrocytes . - pubmed
Furthermore , we show by immunofluorescent labeling that beta - catenin is re - localized on the level of the Golgi apparatus of Schwann cells after incubation with 25 - OH . - pubmed
Median and mean ion levels were low for unilateral resurfacings ( Cr : median 1.3 microg / l , mean 1.95 microg / l ( & lt ; 0.5 to 16.2 ) ; Co : median 1.0 microg / l , mean 1.62 microg / l ( & lt ; 0.5 to 17.3 ) ) and bilateral resurfacings ( Cr : median 3.2 microg / l , mean 3.46 microg / l ( & lt ; 0.5 to 10.0 ) ; Co : median 2.3 microg / l , mean 2.66 microg / l ( & lt ; 0.5 to 9.5 ) ) . - pubmed
The ratio of kaempferols to apigenins of the exudate also shows seasonal variation ( maximum value in summer and minimum in spring ) . - pubmed
Galactose modified trimethyl chitosan - cysteine ( GTC ) conjugates with various galactose grafting densities were developed for oral delivery of Survivin shRNA - expression pDNA ( iSur - pDNA ) and vascular endothelial growth factor ( VEGF ) siRNA ( siVEGF ) in the synergistic and targeted treatment of hepatoma . - pubmed
Dietary magnesium intake is inversely associated with serum C - reactive protein levels : meta - analysis and systematic review . - pubmed
This post hoc analysis of the Phase III TOWER study evaluated the effects of teriflunomide treatment on five severe relapse outcomes : relapses with sequelae defined by an increase in Expanded Disability Status Scale ( EDSS)/functional system ( FS ) score ( sequelae - EDSS / FS ) 30 days post relapse ; relapses with sequelae defined by the investigator ( sequelae - investigator ) ; relapses leading to hospitalization ; relapses treated with intravenous corticosteroids ; and intense relapses using the definition of Panitch et al . - pubmed
Compared with placebo , teriflunomide significantly reduced annualized rates of relapses with sequelae - EDSS / FS [ 14 mg , 36.6 % ( p = 0.0021 ) ; 7 mg , 31.3 % ( p = 0.0104 ) ] and sequelae - investigator [ 14 mg only , 53.5 % ( p = 0.0004 ) ] , relapses leading to hospitalization [ 14 mg only , 33.6 % ( p = 0.0155 ) ] , relapses requiring intravenous corticosteroids [ 14 mg , 35.7 % ( p = 0.0002 ) ; 7 mg , 21.5 % ( p = 0.0337 ) ] , and intense relapses [ 14 mg only , 52.5 % ( p = 0.0015 ) ] . - pubmed
To examine this effect , distortion product otoacoustic emissions ( DPOAEs ) measurements and serum levels of oxidative and antioxidant status [ including malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione ( GSH ) , glutathione peroxidase ( GPX ) , total oxidant status ( TOS ) , total antioxidant status ( TAS ) , and oxidative stress index ( OSI ) ] were evaluated . - pubmed
We showed that in cultured microglia , activators of Nrf2 ( i ) induce antioxidative defense components , ( ii ) reduce peroxide formation , ( iii ) up - regulate phagocytosis - mediating scavenger receptor CD36 , and ( iv ) enhance red blood cells ( RBC ) phagocytosis . - pubmed
Several highly conserved amino acid residues were found high in p110 beta ( n = 102 ) . - pubmed
Subsequently , the number of highly conserved amino acid restudies was low in p50alpha and p55gamma ( n = 15 ) . - pubmed
This interaction , mainly in groove mode , weakens the hydrogen bonds between base pairs of the helix inducing denaturation of the double strands , as well as condensation of the macromolecule , from an extended coil state to a globular state . - pubmed
A pooled analysis of six randomized placebo - controlled trials ( n=618 ) indicated that responses at 4 weeks may predict response at 6 - 9 weeks , although another pooled analysis of open - label data ( n=338 ) suggests that the probability of a robust response to atomoxetine [ & gt;=40 % decrease in ADHD - Rating Scale ( ADHD - RS ) scores ] may continue to increase beyond 6 - 9 weeks . - pubmed
We have previously reported that the mechanisms underlying the effects of caffeine , a non - selective adenosine A1 / A2 receptor antagonist , combined with oseltamivir . - pubmed
These enhancements were inhibited by an adenosine A2 receptor agonist , CGS21680 ( 0.2 mg / kg , subcutaneously ( s.c . ) - pubmed
Furthermore , an adenosine A2 receptor antagonist , SCH58261 ( 1 and 3 mg / kg , i.p . ) - pubmed
Our findings suggest an interaction between the central blockade of adenosine A2 receptors by caffeine and OCB - induced behavioral changes . - pubmed
The results indicated that crosslinked NQC / PVA hydrogels with glutaraldehyde ( GA ) are more thermallystable than non crosslinked hydrogels , NQC / PVA hydrogels swell highly in different buffer solutions as PVA content increases and the antimicrobial activity of NQC / PVA hydrogels is higher than NQC itself . - pubmed
Maternal separation had opposite effects on NMDA receptor - mediated inhibition of norepinephrine release in SHR and WKY hippocampus , as it increased inhibition of both glutamate - stimulated and depolarization - evoked release in SHR hippocampus but not in WKY . - pubmed
Nucleotide excision repair ( NER ) removes chemically diverse DNA lesions in all domains of life . - pubmed
These steps are coordinated by ATP binding and hydrolysis in the ' proximal ' and ' distal ' UvrA ATP - binding sites . - pubmed
Subsequent UvrB recruitment requires ATP hydrolysis in the proximal site . - pubmed
Among 22 transfer RNAs , 21 have the typical clover - leaf structure , while tRNASer ( AGN ) does not possess the dihydrouridine ( DHU ) arm and could not form a stable stem - loop structure . - pubmed
Inositol features may exert insulin - like effects . - pubmed
To determine the type of cholera toxin subunit B ( ctxB ) , nucleotide sequencing was performed . - pubmed
RESULTS : All the eight strains , except one strain , showed resistant to nalidixic acid , sulphamethoxazole , streptomycin and trimethoprim and possessed the sullI , strB , dfrA1 and int SXT genes . - pubmed
This study tested the hypothesis that prenatal HS may influence Adenosine - induced vasodilatation via protein kinase A ( PKA ) pathway in coronary arteries . - pubmed
Coronary arteries from male adult offspring were tested for K + channels and Adenosine signal pathways . - pubmed
After pretreatment with PKA inhibitor , vasodilatation to Adenosine was decreased to a smaller extent in HS than that in control . - pubmed
Forskolin ( activator of adenylate cyclase)-mediated vasodilatation was decreased in HS . - pubmed
Iberiotoxin ( large - conductance Ca2 + - activated K + channel [ BK channel ] inhibitor ) attenuated Forskolin - induced vasodilatation in control , not in HS group . - pubmed
CONCLUSIONS : Prenatal HS diets altered Adenosine - mediated coronary artery vasodilatation in the offspring , which was linked to downregulation of cAMP / PKA / BK channel pathway . - pubmed
UV - B Inhibits Leaf Growth through Changes in Growth Regulating Factors and Gibberellin Levels . - pubmed
Interestingly , the decrease in the GZ size correlates with a decrease in the concentration of GA19 , the immediate precursor of the active gibberellin , GA1 , by UV - B in this zone , which is regulated , at least in part , by the expression of GRF1 and possibly other transcription factors of the GRF family . - pubmed
Mutations in the beta hairpin of ribosomal protein uL22 , which is rather distal to the erythromycin binding site , also generate resistance to the antibiotic . - pubmed
Hydrogen saline suppresses neuronal cell apoptosis and inhibits the p38 mitogen - activated protein kinase - caspase-3 signaling pathway following cerebral ischemia - reperfusion injury . - pubmed
These results demonstrated that H2 significantly reduced the number of apoptotic cells , and the protein expression of p38 MAPK and caspase-3 , compared with the IR group . - pubmed
Folate plus zinc supplementation did not show any statistically different effect on serum testosterone ( P = .86 ) , inhibin B ( P = .84 ) , FSH ( P = .054 ) , and sperm motility ( P = .169 ) as compared to the placebo . - pubmed
Potential immunotoxic effects of trichloroethylene - induced IV allergic reaction in renal impairment . - pubmed
A high degree of cytokine deposition was observed in the renal tubular proximal epithelial cells in the TCE - sensitized groups . - pubmed
The expression levels of genes involved in inflammatory signaling ( TNFAIP2 , ACOT7 , and RORA ) were also associated with birth weight , suggesting a role for inflammatory processes in Cd - associated reproductive toxicity . - pubmed
All these compounds form hydrophobic interaction , aromatic pi - pi stacking and hydrogen bond efficiently with the Eg5 . - pubmed
The serotonin transporter polymorphism ( 5 - HTTLPR ) and cortisol stress responsiveness : preliminary evidence for a modulating role for sleep quality . - pubmed
The short ( S ) allele of a functional polymorphism ( 5 - HTTLPR ) within the promoter region of the serotonin transporter gene ( SLC6A4 ) is found to predispose the risk for stress - related affective disorders relative to the long ( L ) allele . - pubmed
Accounting for this 5 - HTTLPR - dependent influence enhanced the predictive value of 5 - HTTLPR on cortisol stress responsiveness , revealing greater HPA reactivity in S - allele relative to L - allele carriers . - pubmed
Molecular recognition of RAS / RAF complex at the membrane : Role of RAF cysteine - rich domain . - pubmed
Activation of RAF kinase involves the association of its RAS - binding domain ( RBD ) and cysteine - rich domain ( CRD ) with membrane - anchored RAS . - pubmed
Design , synthesis and antifungal evaluation of novel pyrazole carboxamides with diarylamines scaffold as potent succinate dehydrogenase inhibitors . - pubmed
And compound 1c ( IC50 = 0.034 mg / L ) showed higher inhibition abilities against succinate dehydrogenase than fluxapyroxad ( IC50 = 0.037 mg / L ) . - pubmed
Chemical immunosuppressive drugs including mammalian target of rapamycin inhibitors ( sirolimus and everolimus ) , calcineurin inhibitors ( tacrolimus and cyclosporine ) , and purine and pyrimidine synthesis inhibitors ( 6 - mercaptopurine , mycophenolic acid , and methotrexate ) are widely prescribed for the treatment of autoimmune and inflammatory diseases and for controlling alloimmunity in interfering with the signals that activate and allow T cells to proliferate . - pubmed
We found that isopsoralen pretreatment significantly reversed the increase in DNA fragmentation and apoptosis rate , and significantly decreased the caspase-3 activity and PARP cleavage in IL-1beta - treated chondrocytes . - pubmed
Moreover , the expressions of LC3 - II and LAMP-1 were markedly increased but the expression of p62 / SQSTM1 was remarkably decreased by isopsoralen pretreatment . - pubmed
Importantly , the protective effects of isopsoralen against IL-1beta were blocked by pretreatment with autophagy inhibitor 3 - MA and bafilomycin A1 . - pubmed
Thirty years ago , a novel axis of the renin - angiotensin system ( RAS ) was unveiled by the discovery of angiotensin -( 1 - 7 ) [ ANG -( 1 - 7 ) ] generation in vivo . - pubmed
Very recently , another branch of the RAS was discovered , based on angiotensin peptides in which the amino - terminal aspartate was replaced by alanine , the alatensins . - pubmed
Ala - ANG-(1 - 7 ) or alamandine was shown to interact with Mas - related G protein - coupled receptor D , and the first functional data indicated that this peptide also exerts protective effects in the cardiovascular system . - pubmed
ACD incidence decreased by 53 % ( 95%CI : 0.27 - 0.79 ) per 100 cells / L increase in baseline CD4 - cell count and decreased by 90 % ( 95%CI : 0.01,0.87 ) among adults treated with nevirapine - containing HAART . - pubmed
Glucose Oxidase - Based Glucose - Sensitive Drug Delivery for Diabetes Treatment . - pubmed
The glucose - sensitive drug delivery systems based on glucose oxidase ( GOD ) , which exhibit highly promising applications in diabetes therapy , have attracted much more interest in recent years . - pubmed
The self - regulated drug delivery systems regulate drug release by glucose concentration automatically and continuously to control the blood glucose level ( BGL ) in normoglycemic state . - pubmed
The applications of GOD - immobilized platforms , such as self - assembly layer - by - layer ( LbL ) films and polymer vesicles , cross - linking hydrogels and microgels , hybrid mesoporous silica nanoparticles , and microdevices fabricated with insulin reservoirs have been surveyed . - pubmed
Progression - free survival ( PFS ) superiority with osimertinib over comparator EGFR - TKI remained consistent irrespective of randomization route ( central / local EGFRm positive tissue test ) . - pubmed
In this framework , we report the characterization of Met - Ageritin , an isoform of Ageritin with an additional N - terminal methionyl residue . - pubmed
Met - Ageritin inhibits protein synthesis in vitro with an IC50 = 2.8 nM that is 21 - fold lower than that of Ageritin , while not show endonuclease activity on DNA . - pubmed
In contrast , reducing nicotine by reducing number of cigarettes per day ( CPD ) is common . - pubmed
Participants were then randomized to gradually reduce to 70 % , 35 % , 15 % , and 3 % of baseline nicotine over 4 weeks by either ( 1 ) transitioning to lower nicotine VLNC cigarettes or ( 2 ) reducing the number of full nicotine CPD . - pubmed
CONCLUSIONS : Reducing number of CPD increased QAs more than reducing nicotine via switching to VLNC cigarettes . - pubmed
IMPLICATIONS : Reducing the frequency of smoking behavior ( ie , CPD ) could be a more effective strategy to increase QAs than reducing the magnitude of nicotine in each cigarette ( ie , VLNC ) per se . - pubmed
The MoS2@polypyrrole@Fe3O4 composite was skillfully synthesized via a novel one pot method . - pubmed
In addition , the one pot method used in this work can promisingly be applied to prepare other similar composites such as carbon fiber@polypyrrole@Fe3O4 , graphene@polypyrrole@Fe3O4 . - pubmed
The liver is enriched with polyunsaturated triglycerides ( PUFA - TGs ) in PNPLA3 - I148 M carriers . - pubmed
RESULTS : PUFA - TGs were significantly increased in AT in carriers versus non - carriers of PNPLA3 - I148M. - pubmed
PNPLA3 - I148 M locally remodels AT TGs to become polyunsaturated as it does in the liver , without affecting lipolysis or composition of serum NEFAs . - pubmed
We measured iPTH , oxPTH and n - oxPTH at study entry in 600 kidney transplant recipients ( KTRs ) . - pubmed
Length of root and hypocotyl was enhanced by BS compared to CS silver NPs . - pubmed
From proteomics , abundances of peroxidases were increased under CS silver NPs . - pubmed
Immunoblot analysis depicted that accumulation of ascorbate peroxidase , glutathione reductase , and peroxiredoxin remained unchanged under both BS and CS silver NPs . - pubmed
ATP content decreased under CS silver NPs compared to BS silver NPs . - pubmed
Length of root and hypocotyl was enhanced by GS silver NPs compared to CS silver NPs . - pubmed
From the proteomics , the abundances of peroxidases were increased under CS silver NPs . - pubmed
Immunoblot analysis depicted that accumulation of ascorbate peroxidase , glutathione reductase , and peroxiredoxin remained unchanged under both GS and CS silver NPs . - pubmed
ATP content decreased under CS silver NPs compared to GS silver NPs . - pubmed
The 26S proteasome is a 2.5 - MDa protease complex responsible for the selective and ATP - dependent degradation of ubiquitylated proteins in eukaryotic cells . - pubmed
Accompanied by deciphering the structural diversity of ubiquitin chains with eight linkages and chain lengths , the ubiquitin code for proteasomal degradation has been expanding beyond the best - characterized Lys48 - linked ubiquitin chains . - pubmed
One of the latter ( rs13266634 ) locates in a SLC30A8 exon , encoding a tryptophan - to - arginine substitution that decreases SLC30A8 function , being the canonical explanation for type 2 diabetes risk association . - pubmed
METHODS : This multicenter prospective study involved 25 cases of fungal keratitis that were treated by DALK with cross - linked APCSs and post - operative topical tacrolimus from December 2013 to November 2014 at the Wenzhou Eye Hospital and the Henan provincial Eye Hospital . - pubmed
Post - operative topical tacrolimus alleviated the ciliary injection , except in one case which acute stromal rejection occurred . - pubmed
CONCLUSIONS : DALK using cross - linked APCS combining topical tacrolimus treatment is safe and effective in managing fungal keratitis . - pubmed
Tauroursodeoxycholic acid ( TUDCA ) , an inhibitor of ERS , or vehicle was administered intracerebroventricularly for 4 weeks post - HF induction . - pubmed
Analysis of rostral ventrolateral medulla gene expression confirmed the presence of ERS , inflammation , and activation of renin - angiotensin system pathways in high output HF and showed that TUDCA treatment completely abolished ERS and ERS - related signaling . - pubmed
Response to comments on : Short - term outcome of botulinum neurotoxin A injection with or without sodium hyaluronate in the treatment of infantile esotropia - A prospective interventional study . - pubmed
Regulation of valproic acid induced EMT by AKT / GSK3beta / beta - catenin signaling pathway in triple negative breast cancer . - pubmed
Valproic acid ( VPA ) is a selective histone deacetylation ( HDAC ) inhibitor and exerts anti - cancer properties in different types of cancer . - pubmed
Therefore , we aimed to clarified the unravel relationship between AKT / GSK3beta / beta - catenin signalling pathway and VPA - induced EMT in triple negative breast cancer ( TNBC ) . - pubmed
Our results showed that VPA remarkably inhibited the growth of TNBC cell and triggered apoptotic cell death through G0 / G1 arrest . - pubmed
Furthermore , VPA increased cell migration and activated the EMT process through significantly increasing Snail expression and in turn downregulation of E - cadherin and GKS3beta levels . - pubmed
However , the level of AKT and beta - catenin was reduced after treatment of VPA . - pubmed
Our data showed that VPA induced EMT process and cell migration in TNBC cells . - pubmed
In Situ Exosomal MicroRNA Determination by Target - Triggered SERS and Fe3O4@TiO2 - Based Exosome Accumulation . - pubmed
The most common adverse events were fatigue ( 12.6 % ) , pruritis ( 7.3 % ) , increased serum ALT ( 4.7 % ) and bilirubin ( 3.8 % ) levels , and anemia ( 3.1 % ) . - pubmed
This contrast allows accurate identification of fluorescent CSF cells and structures in formalin fixed paraffin embedded brain tissues ; in the latter , the combined optical procedure is fruitfull in order to avoid fluorescent artifacts misinterpretation . - pubmed
NT and NE caused a decrease in serum cholesterol and phospholipid levels , an increase in liver cholesterol level , no significant change in triglyceride levels of both serum and liver , with a significant change in serum lipoprotein patterns ; a decreased in alpha - and beta - lipoproteins and a marked increase in pre beta - lipoprotein . - pubmed
Pure NT decreased serum cholesterol without causing any change in lipoprotein pattern . - pubmed
MAP , CMA and DOCA causee almost no effect on lipid levels in serum and liver , but CMA and DOCA increased alpha - lipoprotein and decreased beta - and pre beta - lipoproteins . - pubmed
An acute treatment with estradiol caused a decrease in alpha - and beta - lipoproteins and an increase in pre beta - lipoprotein with a decrease in serum lipid levels and an increase in liver lipids . - pubmed
Transamination rates of 3 - hydroxy - DL - kynurenine and 5 - hydroxy - DL - kynurenine by the enzyme were 1/2.9 and 1/2.6 of kynurenine . - pubmed
The enzyme showed a higher preference for pyruvate than 2 - oxoglutarate as aminoacceptor . - pubmed
Besides high resistances to adriamycin , actinomycin D , and vincristine , we observed a lowered sensitivity towards bleomycin , a rather hydrophilic drug usually not involved in P - glycoprotein associated MDR . - pubmed
The expression of this virus is regulated by mammary cell type - specific factors , steroid hormones , and polypeptide growth factors . - pubmed
Besides ketanserin , the descending inhibition was blocked by ritanserin , haloperidol , and pipamperone , which have affinities to 5 - hydroxytryptamine2 receptors , and also by spiperone and methiothepin , which are antagonists at both 5 - hydroxytryptamine1 and 5 - hydroxytryptamine2 receptors ( all 1 microM ) . - pubmed
The results with these antagonists favor the involvement of 5 - hydroxytryptamine2 receptors although the results with mesulergine disagree with this notion . - pubmed
5 - Hydroxytryptamine1 agonists , such as 8 - hydroxy-2 -( di - n - propylamino ) tetralin , buspirone , and 5 - carboxyamidotryptamine , and a 5 - hydroxytryptamine3 agonist , 2 - methyl-5 - hydroxytryptamine , induced a long - lasting inhibition of the monosynaptic reflex , which was blocked by ketanserin whereas a 5 - hydroxytryptamine2 agonist , S -(+)- alpha - methyl-5 - hydroxytryptamine , evoked a biphasic inhibition , in which only the later component was blocked by ketanserin.(ABSTRACT TRUNCATED AT 400 WORDS ) - pubmed
Oncoprotein - mediated signalling cascade stimulates c - Jun activity by phosphorylation of serines 63 and 73 . - pubmed
Phosphorylation of these residues , serines 63 and 73 , stimulates the transactivation function of c - Jun and is required for oncogenic cooperation with Ha - Ras . - pubmed
These oncoproteins , v - Sis , v - Src , Ha - Ras , and Raf-1 , participate in a signal transduction pathway that leads to increased phosphorylation of serines 63 and 73 on c - Jun. While oncogenic Ha - Ras is a constitutive stimulator of c - Jun activity and phosphorylation , the normal c - Ha - Ras protein is a serum - dependent modulator of c - Jun ' s activity . - pubmed
Support for the segregation of NMDA and non - NMDA receptors in discrete anatomical patches arises from the observation that the fast and slow components of dual - component potentials mediated via NMDA and non - NMDA receptor types can fail independently . - pubmed
We report the occurrence of spontaneous TTX - resistant EPSPs in sensory interneurons that are mediated by EAA receptors . - pubmed
These data suggest that spontaneous EPSPs reflect release of EAA neurotransmitter at synaptic contacts overlying homogeneous populations of either NMDA or non - NMDA receptors . - pubmed
Evaluation of a multicentre study with Iomazenil -- a benzodiazepine receptor ligand . - pubmed
After showing in an earlier publication that Iomazenil is a potent benzodiazepine receptor antagonist , the substance has been distributed to 11 clinical centres in Europe for further tests . - pubmed
Prior to the Iomazenil examination , flow images by perfusamine or HMPAO were required , and as comparative methods EEG , computed tomography ( CT ) and magnetic resonance imaging ( MRI ) were performed . - pubmed
In vitro studies indicate that in the absence of sodium chloride , compounds 3a , 4a , N - propyl - N - norbuprenorphine ( 5 ) , buprenorphine and diprenorphine are reasonably comparable in binding affinity for opioid receptors . - pubmed
The endothelium - derived relaxing factor generated by bradykinin in this vascular bed is an oxygen radical generated in association with enhanced arachidonate metabolism via cyclooxygenase . - pubmed
Following intraarterial ( IA ) or intravenous ( IV ) administration of cisplatin ( 2.5 mg / kg ) , changes in the tumor cells were examined electron microscopically . - pubmed
Assessments were made of sinus node function , intra - atrial , atrioventricular ( AV ) nodal and His - Purkinje conduction , and atrial and ventricular refractoriness in the control state and during RVS and LVS at 4 - 12 Hz . - pubmed
Long term haemodynamic , pulmonary function and symptomatic effects of pirbuterol in COPD . - pubmed
In the absence of UV irradiation , m - ATIP inhibited K+ -stimulated ATPase activity in lyophilized gastric vesicles competitively with respect to K+ , with a Ki value of 2.4 microM at pH 7.0 . - pubmed
Irradiation of lyophilized gastric vesicles at pH 7.0 with [ 14C ] m - ATIP in the presence of 0.2 mM ATP resulted in a time - dependent inactivation of ATPase activity that was associated with an incorporation of radioactivity into a 100 - kDa polypeptide representing the catalytic subunit of the ( H + + K +)- ATPase . - pubmed
A mechanism by which nitrate receptors could be manipulated to increase vascular responsiveness is theorized , as well as a means by which a nitrate - free interval might avoid the development of hemodynamic attenuation in terms of cellular mechanisms and receptors . - pubmed
Mitochondrial succinate dehydrogenase activity was decreased in the presence of MEHP . - pubmed
Pulmonary function was evaluated by means of spirometry and a single breath nitrogen washout . - pubmed
Following the introduction of chlormethiazole , there was a significant reduction in DT mortality and in duration of DTs . - pubmed
Glucose and insulin levels in obese non - diabetics . - pubmed
[ Two - dimensional electrophoresis of the products of limited hydrolysis of human serum albumin using isoelectric focusing in a borate - polyol system ] . - pubmed
A procedure developed involved isoelectrofocusing in borate - polyol system and thin - layer electrophoresis in polyacrylamide gel gradient containing sodium dodecylsulfate of the products of human blood serum albumin obtained after partial hydrolysis . - pubmed
Mean serum total cholesterol concentration was 5.7 mmol / l ; about 60 % of this cholesterol was located in the HDL fraction ( density greater than 1.063 g / ml ) . - pubmed
The LDL ( defined as heparin - manganese precipitable lipoproteins in VLDL and chylomicron - deficient serum ) contained 49 % of cholesterol and 43 % of phospholipids ( on the basis of total LDL lipids ) . - pubmed
The HDL ( defined as heparin - manganese soluble lipoproteins in VLDL and chylomicron - deficient serum ) contained 36 % of cholesterol and 58 % of phospholipids ( on the basis of total HDL lipids ) . - pubmed
[ Conformational changes in ionic pump structure during interaction of Na plus - K plus - ATPase with ouabain ] . - pubmed
[ Diffusion procedures for the determination of ammonia within the framework of the post mortem diagnosis of uremia ] . - pubmed
[ Actinomycin D derivatives that recognize 2 DNA GC pairs : bis - actinomycin D ] . - pubmed
In both WR and WD subjects selective unloading of cardiac baroreceptors with lower body negative pressure ( LBNP ) -15 mmHg for 30 min did not increase AVP levels . - pubmed
Further analysis of the data based on serum osmolalities ( OSM ) indicated that LBNP -40 mmHg significantly increased AVP levels ( 3.72 + /- 0.43 to 13.59 + /- 3.63 microU / ml ; P less than 0.05 ; n = 9 ) during sessions with OSM greater than 294 mosmol / kg , but not during sessions with OSM less than 294 mosmol / kg ( 2.85 + /- 0.29 to 4.66 + /- 1.15 microU / ml ; P = NS ; n = 11 ) . - pubmed
Gas chromatography of the acid - hydrolyzed protein confirmed the presence of fatty acid covalently associated with the protein presumably through an amino linkage . - pubmed
Digestion of the fatty acid - containing peptide with pronase resulted in the fatty acid together with L - alanine becoming extractable into hexane . - pubmed
Overall , the prevalence of autoantibodies against thyroid peroxidase , thyroglobulin , thyroxine , and triiodothyronine was 45 % , 18 % , 42 % , and 36 % , respectively . - pubmed
Two of these are in the helix 1A subdomain of the type II keratin 1 , giving a serine - to - proline substitution in codon 185 and an asparagine - to - serine substitution in codon 187 . - pubmed
In the analogous region of type I keratin 10 , an arginine - to - proline and an arginine - to - serine transition in codon 156 have been identified . - pubmed
Intracerebroventricular injection of capsaicin and SP antiserum had no effect on the immune response to SRBC . - pubmed
Treatment with retinoic acid ( RA , 100 nM ) increased medium concentrations of IGFBP-3 to 175 + /- 8 % ( mean + /- SE , n = 4 ) , and IGFBP-6 to 217 + /- 20 % of control values . - pubmed
In the presence of 100 nM RA , the stimulation elicited by these agents was enhanced , with IGFBP-3 levels increasing to 6 - fold above that seen with RA alone . - pubmed
IGFBP-6 increased 12 - fold with RA + forskolin and 20 - fold with RA + dbcAMP . - pubmed
Estrogen ( 10 nM estradiol ) reduced basal IGFBP-3 levels by 25 % but increased IGFBP-6 1.5- to 2 - fold . - pubmed
The stimulatory effect of RA + forskolin on IGFBP-3 was partially reversed by estrogen , whereas RA + forskolin - stimulated IGFBP-6 levels were further increased by estrogen . - pubmed
Increased IGFBP-3 and - 6 production in response to RA + forskolin was accompanied by a decrease in IGF - stimulated thymidine incorporation into DNA ; by contrast , the bioactivity of an IGF analog that does not bind with IGFBPs , [ Gln3 , Ala4 , Tyr15 , Leu16]IGF - I , was unchanged under these conditions . - pubmed
Further , IGFBP-3 and IGFBP-6 are differentially regulated by estrogen . - pubmed
However , all of these studies have been limited to the use of exogenous reagents to perturb GalTase function . - pubmed
Tn and sialyl - Tn carbohydrate structures , first identified in glycophorins of persons with the rare Tn syndrome , were found to be present on the surface of most cancer cells . - pubmed
In this article , the studies on Tn and sialyl - Tn antigens as diagnostic and prognostic tumor markers and as immunogens in vaccines for cancer immunotherapy are shortly reviewed . - pubmed
Biochemically , there was increase of AST , ALT and cholesterol in 48 % , of GGT in 52 % and of alkaline phosphatase in 38 % . - pubmed
The effects of free radicals on voltage - gated Ca2 + currents ( ICa ) were investigated in single guinea - pig ventricular myocytes using the whole - cell clamp technique . - pubmed
ICa was measured in the baseline state and after the application of free radicals from cumene hydroperoxide or generated from the addition of purine to xanthine oxidase . - pubmed
ICa also decreased from 1303 + /- 560 pA to 965 + /- 360 pA in the presence of the free radical generating system ( 2.3 mM purine plus 20 U / l xanthine oxidase ) . - pubmed
The administration of CaNa2EDTA or DMSA was more effective than that of DMPS , and the combination of CaNa2EDTA and DMSA was more efficient than that of CaNa2EDTA and DMPS or the individual chelators in enhancing urinary / fecal excretion of lead , in reducing hepatic , renal , and femur concentration of lead , and in restoring lead - induced inhibition of blood delta - aminolevulinic acid dehydratase and increase in blood zinc protoporphyrin and urinary delta - aminolevulinic acid levels . - pubmed
We now found that CK II is activated only when the cells are exposed to both IGF-1 and TF or when TF is replaced in this combination with relatively high levels of iron salts . - pubmed
Using cells either wild type for acetyl phosphate metabolism or defective for phosphotransacetylase or acetate kinase , or both , we measured flagellar expression and the intracellular concentration of acetyl phosphate relative to growth phase and temperature . - pubmed
To extend these observations , we measured the intracellular concentration of acetyl - CoA , the level of expression from the pta and ackA promoters , and the activities of phosphotransacetylase and acetate kinase derived from cell lysates . - pubmed
Relative to increasing culture density , acetyl - CoA levels and expression from both the pta and ackA promoters decreased . - pubmed
We propose that cells regulate intracellular acetyl phosphate concentrations relative to growth phase and temperature by modulating the availability of acetyl - CoA , the expression of ackA , and the activity of phosphotransacetylase . - pubmed
This selective activation suggests that the mechanism of action of pentylenetetrazol , as a convulsant , is not simply as an antagonist of GABA receptors . - pubmed
METHODS : Renal function was assessed by S - creatinine and the albumin / creatinine clearance ratio , and arthritic activity by joint score and C - reactive protein ( CRP ) . - pubmed
Prompt institution of corticosteroid treatment is regarded as indispensable at increase of CRP / S - AA due to infection or surgery . - pubmed
Our data suggest a role for immunoregulatory cytokines and prostanoids such as IL-1 beta and PGE2 in these interactions . - pubmed
Carboxy terminal sequence and synthesis of rat kidney laminin gamma 1 chain . - pubmed
N - Ethylmaleimide inhibited alternative oxidase activity , but iodoacetate was found to stimulate activity much like pyruvate , including enhancing the enzyme 's apparent affinity for reduced ubiquinone . - pubmed
The presence of pyruvate or iodoacetate blocked inhibition of the enzyme by N - ethylmaleimide , indicating that all three compounds acted at the same sulfhydryl group on the alternative oxidase protein . - pubmed
The site of pyruvate and iodoacetate action was shown to be a different sulfhydryl than that involved in the redox - active regulatory disulfide bond , because iodoacetate bound to the alternative oxidase at the activating site even when the redox - active regulatory sulfhydryls were oxidized . - pubmed
Given the nature of the covalent adduct formed by the reaction of iodoacetate with sulfhydryls , the activation of the alternative oxidase by alpha - keto acids appears to involve the formation of a thiohemiacetal . - pubmed
Dihydrobenzophenanthridine ( DHBP ) oxidase catalyses the last step in the biogenesis of the benzo [ c ] phenanthridine alkaloid sanguinarine . - pubmed
Enzyme - specific activity could be induced in a dose- and time - dependent manner up to 4- to 14 - fold , respectively , when cells were treated with MeJ or with ASA . - pubmed
canadensis defence against pathogens by eliciting the enzymes involved in the synthesis of the phytoalexin benzophenanthridine alkaloids . - pubmed
Castasterone , 6 - deoxocastasterone , typhasterol and 6 - deoxotyphasterol were identified by GC - MS . - pubmed
The 45Ca2 + release was not inhibited by heparin , an antagonist of IP3 receptor . - pubmed
The effects of caffeine , ryanodine and cADPR on 45Ca2 + release were also tested in the presence of thapsigargin ( TG ) , an inhibitor of microsomal Ca2 + - ATPase . - pubmed
The properties of the ryanodine - sensitive mechanism are similar to that of the ryanodine receptor as described in muscle cells . - pubmed
Two of the 16 subjects had familial ALS and mutations in the gene for Cu / Zn SOD1 . - pubmed
Using 3H - daunomycin ( 3H - DM ) and calcein as MRP substrates and probenecid ( PRO ) , genistein ( GEN ) , benzbromarone ( BB ) , N - ethylmaleimide ( NEM ) and verapamil ( VP ) as MRP modulators , drug accumulation studies revealed a transporting activity of MRP that correlated significantly with the gene expression data . - pubmed
Moreover , a significant correlation between MRP expression and chemoresistance against daunomycin ( DM ) , doxorubicin ( DOX ) , etoposide ( VP-16 ) and vinblastine ( VBL ) , but not cisplatin ( CDDP ) and bleomycin ( Bleo ) ( MTT - based survival assay ) , was detected . - pubmed
Synthesis and radiolabeling of ( S )- 4 - amino-5 - iodo-2 - methoxy - N -( 1 - azabicyclo [ 2.2.2 ] oct-3 - yl ) benzamide , the active enantiomer of [ 125I ] iodozacopride , and re - evaluation of its 5 - HT3 receptor affinity . - pubmed
We report an improved synthesis of unlabeled ( S )- iodozacopride , the radiolabeling of ( S )-[ 125I ] iodozacopride via deschloro -( S )- zacopride , and a re - evaluation of its affinity for the 5 - HT3 receptor . - pubmed
The cDNA encoding the 86 kDa arginine kinase was amplified by PCR and the cDNA - derived amino acid sequence of 724 residues was determined . - pubmed
The amino acid sequence clearly indicates that Pseudocardium arginine kinase has a two - domain structure : the first domain residues 1 - 363 and the second domain 364 - 724 . - pubmed
Comparison of the amino acid sequence in the GS region ( a possible candidate for the guanidine substrate recognition site in the phosphagen kinase family ) suggests that the first domain of Pseudocardium arginine kinase might not retain a complete enzyme activity , because the Asp-7 in the GS region , which is assumed to be involved in the recognition of the positive charge of arginine , was replaced by a Gly residue in the first domain . - pubmed
Effects of extracellular acetylcholine on muscarinic receptor binding assessed by [ 125I ] dexetimide and a simple probe . - pubmed
[ 125I ] Dexetimide reached a plateau at 90 min post - injection . - pubmed
After the protein was isolated and the N - terminal amino acid sequence determined , a protein database analysis revealed that it is high mobility group-1 ( HMG-1 ) protein . - pubmed
The interactions with the transition - state analogues are , in general , very similar to those seen previously for the complex of the enzyme with a bestatin - based inhibitor . - pubmed
These observations tend to support one of the mechanistic schemes for eMetAP considered before , although with a revision in the role played by His79 . - pubmed
Since a sub - population of NPY - producing neurons in the arcuate nucleus ( ARC ) of the hypothalamus co - express GABA , the possibility of an interplay between NPY and GABA in the release of LH was investigated in two ways . - pubmed
These morphological findings raised the possibility that inhibitory GABA co - released with NPY may act to restrain the excitatory effects of NPY on LH release . - pubmed
On the other hand , administration of MUS blocked the NPY - induced stimulation of LH release in a dose - dependent manner . - pubmed
Similarly , administration of MUS abolished the excitatory effects on LH release of 1229U91 , a selective NPY Y4 receptor agonist . - pubmed
These results support the possibility that in the event of co - release of these neurotransmitters / neuromodulators , GABA may act to restrain stimulation of LH release by NPY during the basal episodic and cyclic release of LH in vivo . - pubmed
[ Changes in synthesis of nitric oxide , blood levels of ACTH and cortisol in viral hepatitis B ] . - pubmed
ACTH and hydrocortisone blood levels were significantly high in AVH - B and in CH - B exacerbation . - pubmed
A negative correlation between nitrituria / nitraturia and ALAT , ASAT , bilirubin , alkaline phosphatase , gamma - glutamyl - transpeptidase is an indirect evidence for a protective role of NO against viral hepatitis B. - pubmed
The performance of silica gel based cation - exchangers such as BioSil CAT is in most cases better than observed for sulfoacylated resins . - pubmed
The enzyme activity of CYP2C9 catalyzing oxidation of tolbutamide to hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC ) . - pubmed
RESULTS : The amino acid sequence predicted from the cDNA segment was identical to that of CYP2C9 * 1 , the wild type CYP2C9 . - pubmed
21T>C , 1146C>T , but the encoding amino acid sequence was the same , L7 , P382 . - pubmed
One hundred and eighty - five conventional fatty acids were identified by GC - MS , out of which five were new multibranched polyunsaturated fatty acids . - pubmed
The 3 - hydroxy-3 - methylglutaryl ( HMG )- CoA reductase inhibitors or statins are potent inhibitors of cholesterol synthesis . - pubmed
Microarray - based analysis of anti - angiogenic activity of demethoxycurcumin on human umbilical vein endothelial cells : crucial involvement of the down - regulation of matrix metalloproteinase . - pubmed
Left hemihepatectomy ( LHX ) was performed without in situ perfusion ( control - LHX , n = 5 ) or with concomitant in situ perfusion with hypothermic ( 4 degrees C ) Ringer - glucose ( cold - LHX , n = 5 ) or normothermic ( 38 degrees C ) Ringer - glucose ( warm - LHX , n = 5 ) . - pubmed
Permeabilization in a cerebral endothelial barrier model by pertussis toxin involves the PKC effector pathway and is abolished by elevated levels of cAMP . - pubmed
Cellular intoxication by pertussis toxin proceeds via ADP - ribosylation of alpha - G ( i ) proteins , which not only interferes with the homeostatic inhibitory regulation of adenylate cyclase stimulation but also results in a modulation of the membrane receptor coupling . - pubmed
Increasing intra - endothelial cAMP levels by employing cholera toxin or forskolin even inhibited the pertussis toxin - induced permeabilization of endothelial barriers . - pubmed
In summary , we conclude that permeabilization of cerebral endothelial monolayers by pertussis toxin does not depend on elevated cAMP levels and proceeds via the phosphokinase C pathway . - pubmed
However , the BIS values on awakening were highly variable , suggesting that it reflects both the residual depressant effects of methohexital and post - ictal depression . - pubmed
IMPLICATIONS : The bispectral index ( BIS ) value immediately before the electroconvulsive therapy ( ECT ) stimulus correlates with the duration of the motor and electroencephalogram ( EEG ) seizure activity during methohexital anesthesia . - pubmed
[ Effect of cardiac glycosides on actomyosin . - pubmed
[ SPECTROPHOTOMETRIC METHOD FOR THE DETECTION OF NITRIC ACID AND HYDROCHLORIC ACID IN THE PRESENCE OF NITRATES AND CHLORIDES IN THE AIR ] . - pubmed
ACCELERATING EFFECT OF COPPER ION ON THE REACTIVATION OF REDUCED TAKA - AMYLASE A THROUGH CATALYSIS OF THE OXIDATION OF SULFHYDRYL GROUPS . - pubmed
[ TREATMENT OF CORONARY CIRCULATORY DISORDERS WITH ISOPTIN IN GENERAL PRACTICE ] . - pubmed
Lifestyle changes as recommended in diabetes are fundamental for treatment ; addition of insulin - sensitising agents ( eg , metformin ) may be valuable in circumstances such as anovulatory infertility . - pubmed
Infertility can be treated successfully in most women by diet and exercise , clomiphene citrate with or without metformin , ovarian drilling , or ovulation induction with gonadotrophins ; in - vitro fertilisation should be avoided unless there are other indications . - pubmed
Dependence of lipoprotein - lipase - catalyzed triacylglycerol hydrolysis on droplet size of synthetic monodisperse emulsions measured with static light scattering . - pubmed
These emulsions were used as synthetic substrates for lipoprotein lipase , a key enzyme for the hydrolysis of serum triacylglycerols . - pubmed
Our prior studies demonstrated that peri - sciatic zymosan activated macrophages and neutrophils to release proinflammatory cytokines and reactive oxygen species ( ROS ) . - pubmed
A skin prick test , pulmonary function test , histamine challenge test and exercise challenge test ( ECT ) were performed in all subjects . - pubmed
Polymorphisms in ABCG5 and ABCG8 transporters and plasma cholesterol levels . - pubmed
ABCG5 and ABCG8 transporters play an important role in the absorption and excretion of sterols . - pubmed
The Tyr54Cys polymorphism influenced the degree of reduction in total plasma cholesterol ( delta -0.49 mmol / l in Tyr54 homozygotes vs. delta +0.12 mmol / l in Cys54 homozygotes , p<0.04 ) and LDL - cholesterol ( delta -0.57 mmol / l in Tyr54 homozygotes vs. delta +0.04 mmol / l in Cys54 homozygotes , p<0.03 ) levels between 1988 and 1996 in females , but not in males . - pubmed
Male Thr400 homozygotes exhibited a greater decrease in total cholesterol ( delta -0.90 mmol / l vs. delta -0.30 mmol / l , p<0.02 ) and LDL - cholesterol ( delta -0.62 mmol / l vs. delta -0.19 mmol / l , p<0.04 ) than Lys400 carriers . - pubmed
We conclude that Tyr54Cys and Thr400Lys variations in the ABCG8 gene may play a role in the genetic determination of plasma cholesterol levels and could possibly influence the gender - specific response of plasma cholesterol levels after dietary changes . - pubmed
TRPM8 is a TRP family cation channel which can be activated by cold stimuli or l - menthol . - pubmed
[ A new treatment of keratectasia after LASIK by using collagen with riboflavin / UVA light cross - linking ] . - pubmed
This mutation in the conserved cytoskeleton - associated protein , glycine - rich ( CAP - Gly ) domain lowers the affinity of p150Glued for microtubules and EB1 . - pubmed
Cell lines from patients are morphologically normal but show delayed recovery after nocodazole treatment , consistent with a subtle disruption of dynein / dynactin function . - pubmed
In summary , the energy - metabolism oscillation is basically regulated by a feedback loop of oxido - reductive reactions of energy metabolism mediated by metabolites like NADH and ATP , and is modulated by metabolism of storage carbohydrates in combination of post - translational and transcriptional regulation of the related enzymes . - pubmed
Here I propose a model in which an alteration in oxygen metabolism is the key cellular event involved in HIF-1 activation under hypoxic and nonhypoxic conditions . - pubmed
Experimental studies have suggested that angiotensin converting enzyme inhibitors and calcium channel blockers may prevent or arrest progression of renal injury . - pubmed
The positron emission tomography with F18 17beta - estradiol has the potential to benefit diagnosis and treatment of endometrial cancer . - pubmed
BACKGROUND : The positron emission tomography ( PET ) with F18 17beta - estradiol ( FES ) has good imaging for assessment of estrogen receptor in breast cancer . - pubmed
Before hormone treatment was started , FES - PET showed increased uptake of endometrium , magnetic resonance imaging ( MRI ) showed thickness and F-18 fluorodeoxyglucose ( FDG)-PET showed increased uptake . - pubmed
FES - PET after 3 months showed remaining FES uptake , but there were no abnormal findings on MRI and FDG - PET . - pubmed
CONCLUSIONS : To our knowledge , this is the first report that FES - PET has the potential to provide more useful information than did FDG - PET about the hormone therapy . - pubmed
Oxygen sensing and redox signaling : the role of thioredoxin in embryonic development and cardiac diseases . - pubmed
Here we discuss the roles of TRX on oxygen stress and redox regulation from different perspectives , in embryogenesis and in adult diseases focusing on cardiac disorders . - pubmed
Interventional radiology ( IR ) comprising thrombolysis and percutaneous transluminal angioplasty ( PTA ) is well established in the management of thrombosed polytetrafluoroethylene ( PTFE ) grafts . - pubmed
The PKC inhibitor GF-109203x , the MEK inhibitor PD-98059 , and the p38 MAPK inhibitor SB-203580 significantly reduced this contraction . - pubmed
These inhibitory effects were abolished when the microcystin concentration was increased to 10 muM , indicating that application of these kinase inhibitors generated an increase in MLCP activity . - pubmed
On the other hand , SB-203580 , but not GF-109203x or PD-98059 , significantly reduced the phosphorylation level of the PKC - potentiated phosphatase inhibitor of 17 kDa ( CPI-17 ) . - pubmed
A zipper - interacting protein kinase ( ZIPK ) inhibitor ( SM1 peptide ) and a Rho - associated kinase inhibitor ( Y-27632 ) had little effect on microcystin - induced contraction at pCa 9 . - pubmed
In conclusion , PKC , ERK1 / 2 , and p38 MAPK pathways facilitate microcystin - induced contraction at low Ca2 + concentrations by contributing to the inhibition of MLCP activity either through phosphorylation of MYPT1 or CPI-17 [ probably mediated by integrin - linked kinase ( ILK ) ] . - pubmed
The negative regulation of MLCP by PKC and MAPKs during microcystin - induced contraction at pCa 9 , which is not observed in vascular smooth muscle , may be unique to phasic smooth muscle . - pubmed
All NLA derivatives strongly inhibited relaxation of supercoiled DNA catalyzed by either Topo I or II , in a concentration - dependent manner , without stabilization of a cleavable complex . - pubmed
In this study , we examined the effects of Rem , a member of the RGK ( Rem , Rem2 , Rad , Gem / Kir ) family of Ras - related monomeric GTP - binding proteins , on the function of the skeletal muscle L - type Ca ( 2 + ) channel . - pubmed
EC coupling was found to be weakened in myotubes expressing Rem tagged with enhanced yellow fluorescent protein ( YFP - Rem ) , as assayed by electrically evoked contractions and myoplasmic Ca ( 2 + ) transients . - pubmed
This impaired EC coupling was not a consequence of altered function of the type 1 ryanodine receptor , or of reduced Ca ( 2 + ) stores , since the application of 4 - chloro - m - cresol , a direct type 1 ryanodine receptor activator , elicited myoplasmic Ca ( 2 + ) release in YFP - Rem - expressing myotubes that was not distinguishable from that in control myotubes . - pubmed
However , YFP - Rem reduced the magnitude of L - type Ca ( 2 + ) current by approximately 75 % and produced a concomitant reduction in membrane - bound charge movements . - pubmed
Thus , our results indicate that Rem negatively regulates skeletal muscle EC coupling by reducing the number of functional L - type Ca ( 2 + ) channels in the plasma membrane . - pubmed
Blood pressure responses to the nitric oxide synthase inhibitor nitro - L - arginine - methyl - ester were evaluated . - pubmed
CONCLUSION : The present data support the critical role of AT ( 1A ) receptors in the development of 2K1C hypertension , whereas AT ( 1B ) receptors play only a minor role in blood pressure regulation in this model of angiotensin II - dependent hypertension . - pubmed
Activation of AT ( 2 ) receptors does not play an antagonistic role in the AT ( 1 ) receptor - mediated hypertensinogenic actions of angiotensin II in this model . - pubmed
A cholecystokinin-1 receptor agonist ( CCK-8 ) mediates increased permeability of brain barriers to leptin . - pubmed
KEY RESULTS : We observed that CCK-8 depressed the increase in plasma leptin that followed the i.p . - pubmed
The effect of CCK-8 was totally prevented by the CCK1 receptor antagonist , SR-27,897 ( 0.3 mg kg(-1 ) , s.c . ) - pubmed
, but not by the CCK2 receptor antagonist , L-365,260 ( 1 mg kg(-1 ) ) . - pubmed
[ DMF - induced adult hepatocyte apoptosis and its effects on expression of Bcl-2 , Bax and Caspase-3 ] . - pubmed
RESULTS : The increase in apoptotic rate of hepatocytes in concentration - manner was shown after DMF treatment for 12 h. After treatment the expression of Bcl-2 was decreased steadily and lower than the control group ( P & lt ; 0.01 ) , the expression of Bax showed no significant difference among the groups of different dosage by one - factor analysis of variance ( P & gt ; 0.05 ) , as the increase of the dosage of DMF . - pubmed
The ratio of Bcl-2 / Bax dropped with the dosage of DMF increasing , and the ratio in 200 mmol / L of DMF was significantly lower than that of the control ( P & lt ; 0.01 ) . - pubmed
CONCLUSION : DMF can induce apoptosis of cultured adult normal hepatocytes in vitro , and the mechanism might be related to the decrease of Bcl-2 / Bax and the cleavage of Caspase-3 . - pubmed
H2S - donating sildenafil ( ACS6 ) inhibits superoxide formation and gp91phox expression in arterial endothelial cells : role of protein kinases A and G. - pubmed
BACKGROUND AND PURPOSE : Superoxide ( O ( 2 )(*- ) ) , derived from nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase , is associated with acute respiratory distress syndrome ( ARDS ) . - pubmed
NADPH oxidase activity and expression are blocked by nitric oxide ( NO ) and sildenafil . - pubmed
EXPERIMENTAL APPROACH : PAECs were incubated with 10 ng mL(-1 ) tumour necrosis factor - alpha ( TNFalpha ) ( + /-NaHS or ACS6 ) , both of which released H ( 2 ) S , for 2 h or 16 h. O ( 2 )(*- ) was measured . - pubmed
Expression of gp91 ( phox ) was measured by western blotting and the role of cyclic AMP ( cAMP ) and/or cyclic GMP was assessed using protein kinase inhibitors . - pubmed
Both 100 nM NaHS and 1 nM ACS6 completely inhibited gp91 ( phox ) expression induced by TNFalpha . - pubmed
The effects of NaHS were blocked by the inhibition of protein kinase A ( PKA ) , but not PKG , and not by the inhibition of guanylyl cyclase . - pubmed
CONCLUSION AND IMPLICATIONS : H ( 2 ) S inhibited O ( 2 )(*- ) formation and upregulation of NADPH oxidase in PAECs through the adenylyl cyclase - PKA pathway . - pubmed
ACS6 may be effective in treating ARDS through both elevation of cAMP and inhibition of phosphodiesterase type 5 activity . - pubmed
METHODS : Fos immunoreactivity revealing neuronal activation , and degenerating neurons labeled by Fluoro - Jade B ( FJB ) histochemistry were analyzed on the first day after onset of seizures lasting 3 h. Subpopulations of gamma - aminobutyric acid ( GABA)ergic cells were characterized with double Fos - parvalbumin immunohistochemistry . - pubmed
To determine whether the observed bacterial adhesion to fish tissue cells was caused by non - specific interactions , adhesion to bovine serum albumin ( BSA ) and polystyrene was also tested . - pubmed
Our results demonstrated that non - specific adhesion such as hydrophobic interactions are only partially involved in the binding process since adhesion to BSA was low , and there was no correlation between adhesion to polystyrene and adhesion to fish tissue cells . - pubmed
The results showed that a region spanning 11 amino acids ( Ile(72)-Thr(82 ) ) on Glu1 is essential and sufficient for BGAF binding , whereas the extreme N - terminal region Ser(1)-Thr(29 ) , together with C - terminal region Phe(466)-Ala(512 ) , affects the size of Glu1 - BGAF complexes . - pubmed
C-11 binds to BGAF , indicating that the Ile(72)-Thr(82 ) region is indeed a major interaction site on Glu1 involved in BGAF binding . - pubmed
Dexamethasone treatment inhibits VEGF production via suppression of STAT3 in a head and neck cancer cell line . - pubmed
After DEX treatment , the differences of NF - kappaB ( p65 ) , glucocorticoid receptor and p - AKT were not observed between the cell lines . - pubmed
When STAT3 was overexpressed after DEX treatment , the level of VEGF in the culture media was restored . - pubmed
Taken together , we suggest that p - STAT3 can be a mediating factor which regulates VEGF secretion in the DEX treatment . - pubmed
Because the relationship between the three molecules DEX , STAT3 and VEGF is scarcely known , our findings clarified one of the signaling pathways of DEX , which is often used in clinical conditions . - pubmed
MicroRNA-34a is induced via p53 during cisplatin nephrotoxicity and contributes to cell survival . - pubmed
During cisplatin treatment , p53 was activated . - pubmed
Inhibition of p53 with pifithrin - alpha abrogated the induction of miR-34a during cisplatin treatment of proximal tubular cells . - pubmed
Collectively , the results suggest that miR-34a is induced via p53 during cisplatin nephrotoxicity and may play a cytoprotective role for cell survival . - pubmed
Stable and fully functional complexes with different CPR : CYP3A4 stoichiometric ratios are formed within several minutes after addition of the full - length CPR to the solution of CYP3A4 preassembled into POPC Nanodiscs at 37 degrees C. We find that the steady state rates of NADPH oxidation and testosterone hydroxylation strongly depend on CPR : CYP3A4 ratio and reach maximum at tenfold molar access of CPR . - pubmed
Defining the structural requirements for ribose 5 - phosphate - binding and intersubunit cross - talk of the malarial pyridoxal 5 - phosphate synthase . - pubmed
Deletion analysis revealed that the C - terminus has four distinct functionalities : assembly of the Pdx1 monomers , binding of the pentose substrate ( ribose 5 - phosphate ) , formation of the reaction intermediate I(320 ) , and finally PLP synthesis . - pubmed
However , both the native and camphor - soaked forms of CYP101D2 had open conformations with an access channel . - pubmed
Two other potential camphor - binding sites were also identified from electron densities in the camphor - soaked structure : one located in the access channel , flanked by the B / C and F / G loops and the I helix , and the other in a cavity on the surface of the enzyme near the F helix side of the F / G loop . - pubmed
HBsAg positive samples were further tested for IgM anti - HBc , HBeAg and anti - HBe . - pubmed
Specifically , 11.7 % were carriers of HBsAg , whereas 28.8 % were negative for HBsAg but positive for anti - HBc . - pubmed
Among the HBsAg positive patients , 8.5 % were found positive for HBeAg , while 5.1 % was positive for IgM anti - HBc . - pubmed
Endosulfan ( ED ) significantly increased the hemoglobin concentration and white blood cell count after 96 h. Differential leukocytes count was also altered , due to an increase in the percentage of neutrophils in exposed fish . - pubmed
Proinflammatory cytokines , chemokines , adhesion molecules and mediators of inflammation including inducible nitric oxide synthase , cyclooxygenase-2 and nitric oxide orchestrate the inflammatory response . - pubmed
Ginsenosides inhibit different inducers - activated signaling protein kinases and transcription factor nuclear factor - kappaB leading to decreases in the production of cytokines and mediators of inflammation . - pubmed
In this model , d / n - ATF5 expression is controlled by doxycycline and the promoter for GFAP , a marker for stem / progenitor cells as well as gliomas . - pubmed
METHODS : Thirty - one peptides containing 15 amino acids each , which covered the full 222 amino acids of Der p 1 protein sequence , were synthesized on the cellulous membrane by solid - phase peptide ( SPOTs ) synthesis , with 8 overlapping amino acids between every two neighboring peptides . - pubmed
RESULTS : Three strong IgE - binding epitopes were identified in the amino acid sequence of Der p 1 molecule , namely Ep1 ( amino acids 85 - 99 ) , Ep2 ( amino acids 106 - 120 ) and Ep3 ( amino acids 190 - 204 ) . - pubmed
The resulted composites showed uniform palladium nanoparticles distribution , which were characterized by Fourier transform infrared spectrometry ( FTIR ) , transmission electron microscopy ( TEM ) , scanning electron microscopy ( SEM ) , Raman spectroscopy , atomic force microscopy ( AFM ) , X - ray diffraction spectroscopy ( XRD ) and electron diffraction pattern ( ED ) , etc . - pubmed
MATERIALS AND METHODS : The nonfermenters were identified using a standard protocol that included tests for motility , oxidase production , oxidation - fermentation test for various sugars , gelatin liquefaction , and growth on 10 % lactose agar . - pubmed
Influence of proteasome inhibitor bortezomib on the expression of multidrug resistance genes and Akt kinase activity . - pubmed
The goal of this work was to study the mechanisms of ABC family transport proteins ' regulation by a new - generation antitumor drug - the proteasome inhibitor bortezomib ( Velcade ) . - pubmed
We confirmed our previously discovered observation that bortezomib affects the expression of genes involved in the formation of MDR ( ABCB1 gene , also known as MDR1 , and ABCC1 - MRP1 ) , reducing the amount of their mRNA . - pubmed
When exposed to bortezomib , selection of resistant to it cell variants was much faster for a Pgp - overexpressing cell population ( compared to wild - type cells ) . - pubmed
The data indicate that the transcriptional activity of YB-1 might be one of the mechanisms that determine the effect of bortezomib on the amount of MRP1 gene mRNA . - pubmed
Factors associated with OS were bisphosphonates use ( HR = 0.5 , p = 0.008 ) , elevated pre - treatment alkaline phosphatase ( HR = 2.9 , p = 0.003 ) and sunitinib induced HTN ( HR = 0.63 , p<0.0001 ) . - pubmed
Since ethylene receptors negatively regulate signaling , the suppression is canceled upon ethylene binding , permitting responses including fruit ripening . - pubmed
Treatment of preclimacteric fruits with ethylene resulted in accumulation of LeETR4 with reduced phosphorylation whereas treatments of ripening fruits with ethylene antagonists , 1 - methylcyclopropene and 2,5 - norbornadiene , induced accumulation of the phosphorylated isotypes . - pubmed
Alteration in the phosphorylation state of receptors is likely to be an initial response upon ethylene binding since treatments with ethylene and 1 - methylcyclopropene rapidly influenced the LeETR4 phosphorylation state rather than protein abundance . - pubmed
MAIN OUTCOME MEASURES : Lateral thoracolumbar radiographs , bone mineral density ( BMD ) , bone turnover markers , and plasma S1P levels were obtained from all subjects . - pubmed
Induction of heat shock proteins ( HSP ) represents an important adaptive response in muscle subjected to stress , and in several cell types including cardiac myocytes prostaglandins are important in induction of the HSP response . - pubmed
However , the carbohydrate chain heterogeneity on the Fc region of IgG ( Fc - IgG ) can play an important role in modulating the immune response . - pubmed
Altogether , our results show that repeated MDMA exposure causes strong neural and behavioral adaptations and that inhibitory feedback mediated by alpha ( 2A )- adrenergic and 5 - HT ( 1A ) autoreceptors has an important role in the physiopathology of addictive behaviors . - pubmed
The approach is demonstrated in the modification of a quartz surface and in the incorporation of an azobenzene unit into a functional peptide , the third zinc finger in the mammalian factor Sp1 . - pubmed
It is known that the expression of DIO1 , a gene contributing to triiodothyronine ( T3 ) signalling , is regulated by miR-224 . - pubmed
Thus , we analysed mutual regulation between triiodothyronine pathway and miR-224 / miR-452 / GABRE cluster . - pubmed
Firstly , we found that miR-452 directly regulates the expression of thyroid hormone receptor TRbeta1 in renal cancer cells . - pubmed
Previous reports used granulocyte - colony - stimulating factor ( G - CSF ) for mobilization of stem cells ; there are no reported cases undergoing plerixafor mobilization . - pubmed
Since cinnarizine is a well - tolerated calcium channel blocker which has acceptable effect on both vertigo and migraine headache , we carried out this study to evaluate the efficacy and safety of this medication in vestibular migraine and also migraine with brainstem aura associated with vertigo . - pubmed
Development of a Ru complex - incorporated MOF photocatalyst for hydrogen production under visible - light irradiation . - pubmed
A Ru complex - incorporated Ti - based MOF ( Ti - MOF - Ru ( tpy ) 2 ) has been synthesised by using a bis ( 4 '-( 4 - carboxyphenyl )- terpyridine ) Ru ( ii ) complex ( Ru ( tpy ) 2 ) as an organic linker . - pubmed
The solubility of BCS class II compound carvedilol was found to increase significantly in simulated and aspirated media of the fed state . - pubmed
, alpha - , beta - DBE - DBCH , BATE , PBEB , PBT , TBP - DBPE ) were measured close to or below the limit of quantitation ( 0.64 ng / g ) in both the NCCLS and FOX samples , and the seventh , HBB , was measured at median concentrations of 1.85 ng / g and 9.40 ng / g in the NCCLS and FOX samples , respectively . - pubmed
Histone H2B ubiquitination at lysine 120 ( H2Bub1 ) is regulated by RNF20 , an E3 ubiquitin ligase that is altered in many tumor types . - pubmed
Through an evolutionarily conserved trans - histone pathway , H2Bub1 is an essential prerequisite for subsequent downstream dimethylation events at lysines 4 ( H3K4me2 ) and 79 ( H3K79me2 ) of histone H3 . - pubmed
This results in the processing of high - molecular - weight kininogen and the release of bradykinin , a potent vascular mediator . - pubmed
The changes in Tph expression was in agreement with the levels of serotonin ( 5 - HT ) and 5 - hydroxytryptophan in the preoptic area - hypothalamus . - pubmed
Considering the stimulatory influence of 5 - HT on GnRH and GTH release , it is possible to propose a network association between these correlates during early development , which may bring about brain sex dimorphism in males . - pubmed
A recent study from our laboratory during female brain sex development demonstrated high expression of tyrosine hydroxylase in correlation with catecholamine levels , brain aromatase and its related transcription factors such as fushi tarazu factor 1 , Ftz - f1 and fork head box protein L2 , foxl2 . - pubmed
Phase II Trial of S-1 and Oxaliplatin Plus Cetuximab for Colorectal Cancer Patients with Initially Unresectable or Not Optimally Resectable Liver Metastases ( KSCC1002 ) . - pubmed
Overall these studies reveal core mechanisms fundamental to grafted NPC - based therapeutic effects , and delineate the particular contributions of two graft - expressed molecules , SHH and GDNF , in mediating midbrain dopamine neuron protection , and host plasticity after NPC transplantation . - pubmed
The biotransformation of the nanoparticle iron oxide ( maghemite ) core into ferritin , the non - toxic form of iron storage , was demonstrated for the first time ex vivo in atherosclerotic plaques as well as in spleen , the iron storage organ . - pubmed
This study showed that these nanoparticles were stored as non - toxic iron compounds : maghemite oxide or ferritin , which is promising for MRI detection of atherosclerotic plaques in clinics using these USPIOs . - pubmed
Thirteen isolates were found to be ciprofloxacin - resistant , and all harbored a Ser84Leu mutation within the QRDR of the GyrA protein , with 3 isolates showing 2 extra substitutions , Ser98Ile and Arg100Lys ( one strain ) and Glu88Asp and Asp96Thr ( 2 strains ) . - pubmed
The replacement of Asn203 with a basic amino acid was suggested to stabilize alpha - helix 2 , which is important for the Mpr1 structure , probably by neutralizing its dipole . - pubmed
In addition , the combination of two amino acid substitutions at positions 65 and 203 in Mpr1 , Phe65Leu , which was previously isolated by the screening from PCR random mutagenesis library of MPR1 , and Asn203Lys or Asn203Arg , led to further stabilization of Mpr1 . - pubmed
Together , these results suggest that striatal MRS measures of lactate or glutathione might be useful biomarkers for early detection of risk for developing compulsive behavior disorders . - pubmed
These findings suggest that adipocyte COX-2 PGE2 - EP3 - mediated signaling is crucially involved in the development of obesity - associated AT inflammation and insulin resistance.-Chan , P.-C. , Hsiao , F.-C. , Chang , H.-M. , Wabitsch , M. , Hsieh , P. S. Importance of adipocyte cyclooxygenase-2 and prostaglandin E2 - prostaglandin E receptor 3 signaling in the development of obesity - induced adipose tissue inflammation and insulin resistance . - pubmed
Association between estimated blood glucose levels and glycated hemoglobin levels . - pubmed
Tissue specific expression of genes encoding cholesterol transporters ABCA1 and ABCG1 as well as genes encoding the most important transcriptional regulators of adipogenesis - LXRa , LXRb , PPARg and RORa has been investigated in intraabdominal adipose tissue ( IAT ) samples . - pubmed
These data suggest that the nuclear factor RORa can play a significant role in the regulation of cholesterol metabolism and control IAT expression of ABCA1 and ABCG1 , while the level of IAT LXRb gene expression may be an important factor associated with the development of obesity . - pubmed
INTRODUCTION : Anaplastic lymphoma kinase ( ALK ) rearranged lung adenocarcinomas are responsive to the multitargeted ALK inhibitor crizotinib . - pubmed
One of the common mechanisms of resistance to crizotinib is the acquisition of ALK kinase domain mutations . - pubmed
However , the presence of ALK mutations in crizotinib - naive tumors has not been widely reported and it is unclear if de novo ALK mutations affect the response to crizotinib . - pubmed
METHODS : We analyzed preclinical models of ALK rearranged lung cancers that were sensitive / resistant to ALK inhibitors , probed our institutional and other lung cancer databases for tumors with ALK kinase domain mutations , and evaluated tumor response to crizotinib . - pubmed
RESULTS : ALK rearranged cell lines with ALK kinase domain mutations were heterogeneously less inhibited by increasing concentrations of crizotinib than cells driven solely by EML4 - ALK fusions . - pubmed
We identified one TKI - naive ALK rearranged tumor with an ALK kinase domain mutation : ALK - S1206F ( mutations at ALK - S1206 shifted crizotinib inhibitory curves only minimally in preclinical models ) . - pubmed
The never smoker whose tumor harbored de novo EML4 - ALK - E5;A20+ALK - S1206F only achieved a 4 - month radiographic response to crizotinib 250 mg twice daily . - pubmed
In addition to producing antibody , Bregs also regulate diseases via cytokine production and antigen presentation . - pubmed
Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro . - pubmed
We used a combination of Raman microscopy , AFM and TEM to quantify the influence of dimerization on the surface enhanced Raman spectroscopy ( SERS ) signal for gold and silver nanoparticles ( NPs ) modified with Raman reporters and situated on gold , silver , and aluminum films and a silicon wafer . - pubmed
We determined the involvement of afferent sensory neurons , calcitonin - gene - related peptide ( CGRP ) , lipid peroxidation , and nitric oxide ( NO ) biosynthesis in gastroprotection of H2S - releasing NaHS and CO - releasing tricarbonyldichlororuthenium ( II ) dimer ( CORM-2 ) against aspirin - induced injury . - pubmed
The areas of aspirin - induced lesions and gastric blood flow ( GBF ) were assessed by planimetry and laser flowmetry respectively . - pubmed
RESULTS : Aspirin caused gastric lesions , decreased GBF , and raised MDA content , but pretreatment with NaHS and CORM-2 reduced these effects . - pubmed
NaHS and CORM-2 decreased MDA and 4 - HNE content , restoring GPx-1 protein expression . - pubmed
NaHS and CORM-2 prevented aspirin - induced gastric mucosal lipid peroxidation via restoration of microcirculation and antioxidative GPx-1 protein expression . - pubmed
Conversely , the addition of APLN-13 and APLN-17 to in vitro maturation medium containing IGF1 ( 10 - 8 M ) but not FSH ( 10 - 8 M ) arrested most oocytes at the germinal vesicle stage , which was associated with a decrease in progesterone secretion , an inhibition in MAPK ERK1 / 2 phosphorylation and an increase in PRKA phosphorylation in oocytes . - pubmed
Particularly , lupane - type triterpenes have shown great ability to modulate multiple cancer - related signaling pathways and processes , including NF - kappaB , Wnt / beta - catenin , PI3K / Akt , apoptosis , and many other routes related to proliferation or cell death , which are uncontrolled in malignant tumors . - pubmed
NMR data were used to construct a three - dimensional structural model of the CCL2 / CCR218 - 31 complex , revealing that CCR218 - 31 occupies a binding site juxtaposed to the dimer interface , partially replacing monomer - monomer contacts , explaining why CCR218 - 31 binding weakens the dimer interface and induces dissociation . - pubmed
Expert opinion : Baricitinib is an oral targeted synthetic ( ts ) disease - modifying antirheumatic drug ( DMARD ) that mainly inhibits JAK1 and JAK2 . - pubmed
These occurrences are indicative of an expansion of the citrulline - specific B cell response , though ACPA remain of low avidity even in established disease . - pubmed
Before PMX - DHP , the survivor group exhibited a significantly higher PaO2 / FiO2 ( P / F ) ratio and serum surfactant protein - D ( SP - D ) levels and significantly lower lactate dehydrogenase ( LDH ) and ferritin levels than the non - survivor group . - pubmed
Tumor Cell - selective Synergism of TRAIL- and ATRA - induced Cytotoxicity in Breast Cancer Cells . - pubmed
The present work focused on tumor cell - inhibiting effects of a combination of tumor necrosis factor - related apoptosis - inducing ligand ( TRAIL ) and all trans retinoic acid ( ATRA ) in breast cancer cells . - pubmed
Cell viability was assessed via 3 -( 4,5 )- dimethylthiahiazo (- z - yl )- 3,5 - di - phenytetrazoliumromide ( MTT ) assay , the potential of cell colony formation via clonogenic assay , cell death induction via cell - cycle analysis by fluorescence - activated cell sorting ( FACS ) , terminal deoxynucleotidyltransferase - mediated UTP nick end labeling ( TUNEL ) assay and Cell death detection ELISAPLUS , expression of apoptosis and TRAIL pathway proteins via western blot and cell surface expression of TRAIL receptor 1 ( DR4 ) via FACS analysis . - pubmed
RESULTS : TRAIL and ATRA evoked synergistic inhibition of breast cancer cell viability based on cytostatic and cytotoxic mechanisms . - pubmed
CONCLUSION : The cytostatic and cytotoxic effects of the combination of TRAIL and ATRA are tumor cell - selective . - pubmed
Each of NMT and terazosin has its own advantages in attenuating urinary tract irritation and obstruction , but whether their combination may produce a better effect on LUTS and the specific mechanisms of NMT improving acute symptoms of BPH are yet to be further studied . - pubmed
Most frequent pore size in GCT 30 scaffold was the highest of 120 mum whereas that for GCH 30 was the lowest of 96 mum as suggested by Hg porosimetry analysis . - pubmed
Among those in the MAD arm who generated at least trace amounts of urinary ketones , there was a large ( effect size = 0.53 ) and statistically significant ( p = 0.03 ) increase in Memory Composite Score between the baseline and week-6 assessment . - pubmed
The determination of the albumin - creatinine ratio in spot urine is easy to perform and the method of choice to diagnose nephrotic - range proteinuria . - pubmed
Also examined were whether effects on adrenal steroidogenesis that were not obvious in the blood steroid levels after sole administration of KET could be revealed by post - administration of ACTH , and the correlation between the blood and adrenal steroid levels . - pubmed
KET - induced changes in blood levels of other steroids were revealed by ACTH , and the blood and adrenal levels were generally correlated especially after ACTH post - administration . - pubmed
The usefulness of the multiple steroid measurement as a method for mechanism investigation of drug effects on the adrenal gland can be further enhanced by ACTH . - pubmed
In February 2016 , because follow - up CT revealed a right adrenal tumor with time - dependent growth , sunitinib ( 25 mg / body ) was introduced . - pubmed
Vitamin treatments in the commercial diluent were as follows : 2.4 mug of vitamin D3 ( D3 ) or 25 - hydroxylcholecalciferol ( 25OHD3 ) . - pubmed
The ET of eggs significantly fluctuated during the postinjection time period ; however , the type of vitamin D3 source injected did not affect ET . - pubmed
Nevertheless , the injection of 25OHD3 resulted in a lower late embryo mortality than the diluent and D3 injection treatments . - pubmed
In conclusion , the in ovo injection of 25OHD3 has the potential to improve early posthatch broiler performance without affecting ET . - pubmed
The aim of this study is to investigate pharmacokinetic ( PK ) characteristics of inhaled FTS , SM , and TTP after co - administration . - pubmed
A single dose of FTS / SM ( 250/50 microg ) and TTP ( 18 microg ) were concomitantly inhaled in period 1 , and the dose of each drug was escalated to two- and three - fold in periods 2 and 3 , respectively , with a 2 - week washout between periods . - pubmed
In conclusion , PK characteristics of FTS , SM , and TTP by pulmonary absorption were evaluated after concurrent inhalation . - pubmed
In this study , we developed a hydrogel - blended scaffold consisted of gelatin methacryloyl ( GelMA ) and alginate enriched with hydroxyapatite nanoparticles ( HAP ) to model an in vitro prevascularized bone construct . - pubmed
Objectives : C - reactive protein ( CRP ) is an acute phase protein used in multiple canine inflammatory conditions including steroid responsive meningitis - arteritis , immune - mediated polyarthritis and bronchopneumonia . - pubmed
Here a novel MN patch is designed in which the MNs are coated with insulin / poly - l - glutamic acid ( PGA ) layer - by - layer ( LBL ) films at pH 3.0 . - pubmed
The new MN patch is expected to become a painless , convenient method for the control of postprandial glucose . - pubmed
Since HIF-1alpha and VEGFA are critical factors promoting alveolar development and pulmonary angiogenesis , these results suggested that PFOS may also affect lung development by inhibiting HIF-1alpha and VEGFA expression . - pubmed
The primary endpoint was changes in cardio - metabolic risk based on the Suita score at 1 year , and the secondary endpoints were changes in medical examination data related to cardio - metabolic risk ( total cholesterol [ TC ] , triglyceride , high - density lipoprotein cholesterol , low - density lipoprotein cholesterol , body mass index [ BMI ] , and hemoglobin A1c ) at 1 year . - pubmed
The bread was fortified with protein ( whey protein , blood plasma proteins from farm animals ) , dietary fiber , calcium ( eggshell ) , iron ( purified hemoglobin ) and iodized whey protein . - pubmed
Blood levels of total calcium ( by colorimetric method ) , gonadotropins , testosterone , and estradiol ( by enzyme - linked immunosorbent assay ) were analyzed . - pubmed
In animals of the O120 + group , in comparison with the O120 sample , there was a decrease in blood testosterone and a marked compensatory release of follicle - stimulating hormone , while no changes were detected in the concentration of estradiol and the state of the uterus atrophied against the background of ovariectomy . - pubmed
These changes are not specific for acrylamide intoxication , having been observed in other experimentally induced neuropathies ( n - hexane , Mn - BK , CS2 , ... ) , as well as in a variety of diseases both genetically determined and due to exposure to toxic substances ( glue - sniffing , leather cement poisoning , antiblastic therapy , ... ) . - pubmed
A comparative study of antiestrogen action : temporal patterns of antagonism of estrogen stimulated uterine growth and effects on estrogen receptor levels . - pubmed
Changes in the chemical structures of these anti - estrogens produce compounds with markedly different affinities for the uterine estrogen receptor as measured in an in vitro cell - free cytosol system ; binding affinities relative to estradiol ( 100 % ) are : CI-628 , 4 % ; CI-680 , 34 % ; 94X1127 ( 94X),222 % ; U-11,100A ( UA ) , 6 % ; and U-23,469 ( U-23 ) , 0.1 % . - pubmed
The compounds CI-628 , CI-680 and UA ( 50 microng sc in saline ) , which have a methylated hydroxyl group ( at the site analogous to the steroid position 3 ) , show a prolonged maintenance of elevated levels of nuclear receptor ( beyond 48 h ) and elevated uterine weight ( until 72 h ) ; this correlates with a prolonged period of depressed cytoplasmic receptor levels ( beyond 48 h ) and prolonged uterine insensitivity to estrogen ( beyond 36 h as monitored by 3 h wet weight response ) . - pubmed
Comparative studies with these five compounds indicate that they are effective as estrogen antagonists only so long as they maintain cytoplasmic receptor levels low , and that the magnitude of the responsiveness to estradiol after antiestrogen correlates with the extent of reappearance of cytoplasmic receptor . - pubmed
Histamine release is induced by monoclonal antibodies directed against the Fab but not the Fc region of the IgG4 molecule . - pubmed
That HR is calcium dependent and is accompanied by the synthesis and release of leukotriene C4 . - pubmed
In addition , we found that nine donors were unresponsive to an anti - IgE stimulus , while they released histamine efficiently after challenge with anti - IgG4(Fab ) , suggesting the existence of different receptors for both immunoglobulins . - pubmed
Frequent expression of CD19 was found in the blastic population of t(8;21 ) AML ( 18 of 23 cases ) without other B - cell antigens and Ig gene rearrangements . - pubmed
Our findings indicate that leukemic blasts of t(8;21 ) AML commonly express CD19 while preserving the stem cell - associated antigens , and differentiate into the granulocytic pathway with discordant maturation such as low CD33 expression . - pubmed
Neurokinin A ( NKA ) caused dose - dependent increases in ciliary beat frequency ( CBF ) , as measured by a photoelectric method , with the maximal increase from the baseline 15.7 + /- 1.7 % ( mean + /- SEM , p less than 0.01 ) , whereas substance P ( SP ) had no effect . - pubmed
The NKA - induced increase in CBF was not inhibited by phentolamine , propranolol , or atropine , but it was abolished by the tachykinin antagonist [ D - Pro2 , D - Trp7,9 ] SP . - pubmed
Pretreatment of tissue with thiorphan ( 10(-5 ) M ) , a NEP inhibitor , had little effect on CBF responses to NKA ; however , it significantly potentiated the responses to SP ( 14.9 + /- 3.0 % , p less than 0.01 ) . - pubmed
Other peptidase inhibitors , including captopril , bestatin , and leupeptin , did not alter the tachykinin - induced CBF response , suggesting that angiotensin converting enzyme , aminopeptidases , and serine proteinases do not modulate ciliary activity in response to tachykinins . - pubmed
These results suggest that NKA increases CBF by acting directly on tachykinin receptors and that NEP may play a role in modulating the tachykinin - induced stimulatory effects on CBF . - pubmed
Calcitriol as well as angiotensin - converting enzyme could represent unregulated secretion products from granulomatous tissue and their plasma concentrations may roughly reflect activity of the disease . - pubmed
The mechanism of the sensitizer - membrane interactions has been studied by following the distribution properties of selected porphyrins , including haematoporphyrin ( HP ) and protoporphyrin ( PP ) , into unilamellar liposomes of dipalmitoyl phosphatidylcholine ( DPPC ) . - pubmed
At porphyrin concentrations below 0.5 microM , HP and PP exclusively localize in the inner phospholipid monolayer ; at higher concentrations , the outer monolayer also becomes populated . - pubmed
The porphyrin binding sites in liposomes , however , are different for HP and PP : HP preferentially distributes into water - accessible lipid regions , while PP localizes in the most hydrophobic loci of the lipid matrix . - pubmed
In DPPC - cholesterol vesicles , all HP molecules dissolve in DPPC - rich regions while all PP molecules partition in cholesterol - rich environments . - pubmed
While the raised GGT values reduced gradually , the MCV values remained consistently high even after strict withholding of alcohol . - pubmed
We have distinguished the InsP4 binding site from the InsP3 receptor binding site by differences in brain regional and tissue distribution , affinity for InsP4 and InsP3 , and sensitivity to calcium . - pubmed
Secretion of lysosomal beta - glucuronidase , membrane 5 '- nucleotidase , cellular plasminogen activator , and superoxide anion generation were measured as markers of activation . - pubmed
On the basis of the 5'-nucleotidase findings , it is concluded that both the NC and HC granuloma macrophages are highly activated , and further that hypercholesterolemia does not enhance macrophage generation of superoxide anion , either spontaneously or as the result of phorbol myristate acetate stimulation . - pubmed
Hepatocellular and Kupffer cell lysosomes were engorged with ferritin and contained clefts and trilaminar structures believed to represent very long chain fatty acids . - pubmed
Phorbol esters broaden the action potential in CA1 hippocampal pyramidal cells . - pubmed
O , O ' , S - tris - acetyl-3 - thio - hydroquinone , which was characterized by GC / MS . - pubmed
1.5 % of the administered dose of APAP after 24 h , a figure which is equivalent to 6.3 % of the corresponding APAP - thiol conjugates in the urine . - pubmed
The tetrapeptide FMRF - HN2 hyperpolarizes the pacemaker neurons . - pubmed
Identification of a hydroxide ligand at the iron center of ribonucleotide reductase by resonance Raman spectroscopy . - pubmed
Its exact location in the 16O species is obscured by the presence of a protein tryptophan vibration at 758 cm-1 . - pubmed
A model for the binuclear iron center of ribonucleotide reductase is presented in which the hydroxide ligand sites provide an explanation for the half - of - sites reactivity of the enzyme . - pubmed
Rifampicin resistance of RNA polymerase in the binary complex with DNA . - pubmed
The same treatment with TA-064 elevated the concentrations of blood FFA , glycerol and plasma IRI and IRG . - pubmed
These changes induced by TA-064 were inhibited by pretreatment with propranolol ( a non - selective beta - adrenergic antagonist ) and practolol ( a selective beta 1 - adrenergic antagonist ) . - pubmed
They also brought about sustained rises in blood glycerol and plasma IRI , but only transiently increased the plasma IRG level . - pubmed
The cardiotonic agent prenalterol , claimed to be a selective beta 1 - agonist , elevated blood glucose , lactate , and glycerol only slightly , and plasma IRI significantly , but it had no effect on plasma IRG . - pubmed
Stimulation of meiosis resumption in vitro in follicle - enclosed oocytes by both LH and [ D - Ala6 ] GnRH , was blocked by elevated levels of cAMP as demonstrated when either dibutyryl cAMP or the phosphodiesterase inhibitor methylisobutylxanthine was present in the culture medium . - pubmed
On the other hand , the potent GnRH - antagonist [ D - pGlu1 , pClPhe2 , D - Trp3,6 ] GnRH which blocked the stimulatory effect of the agonist on oocyte maturation and ovulation had no effect on LH action . - pubmed
It is concluded that while a GnRH - like peptide does not seem to mediate LH action on the ovarian follicles , both LH and GnRH agonist share some common mechanistic pathways at a post - receptor locus . - pubmed
Since 1 - O - alkyl-2 - lyso - GPC is the metabolic precursor of platelet activating factor , these results further interrelate pathways forming arachidonate metabolites and platelet activating factor ; they also supply a rationale for the observation that both classes of stimuli form concomitantly during cell activation . - pubmed
Experiments designed to test whether Ca2 + mediates metabolic crenation showed that : ( 1 ) an influx of extracellular Ca2 + is not required for metabolic crenation ; ( 2 ) metabolic crenation is accompanied by a 70 % increase in 86Rb + permeability , a change much smaller than the increase expected if crenating concentrations of Ca2 + were released from bound intracellular pools ; ( 3 ) A23187 plus EGTA , a treatment that depletes intracellular Ca2 + and stops Ca2 + crenation , does not affect metabolic crenation ; ( 4 ) calmodulin inhibitors do not slow metabolic crenation . - pubmed
Albumin washes reverse Ca2 + crenation and metabolic crenation involve the accumulation of some amphiphilic species ( e.g. - pubmed
These findings indicate that sulfido derivatives of CP can act directly ( without metabolic activation ) on cells , probably through spontaneous stepwise conversion to phosphoramide mustard , the presumed proximal alkylating agent . - pubmed
Sulfidocyclophosphamide CP derivatives appear to be suitable for in vitro studies of the mechanism of action of CP . - pubmed
Sulfidocyclophosphamide CP derivatives may also have therapeutic potential as CP - like drugs that do not require metabolic activation . - pubmed
The following agents were evaluated in the absence of S9 : 1 - nitropyrene , diesel exhaust extract , and the chlorinated drinking water mutagen 3 - chloro-4 - dichloromethyl-5 - hydroxy-2 -[ 5H ]- furanone ( MX ) . - pubmed
In the presence of S9 , the following agents were evaluated : 4 - aminobiphenyl , benzo [ a ] pyrene , and an organic extract from the particulate emissions resulting from the combustion of polyethylene in a rotary kiln incinerator . - pubmed
MEASUREMENTS : Serum GH and IGF - I were measured by radioimmunoassay whilst uGH was estimated by an immunoradiometric assay using commercially available reagents . - pubmed
Angiotensin - converting enzyme ( ACE ; EC 3.4.15.1 ) may participate in respiratory inflammatory diseases by regulating levels of inflammatory peptides such as bradykinin . - pubmed
ACE activity was significantly more abundant in the membrane - rich fraction than in the soluble cytosolic fraction of nasal mucosal extracts ( 74.18 + /- 24.50 versus 3.99 + /- 1.83 pmol / min / mg protein , respectively , P & lt ; 0.01 by an enkephalin degradation assay ; 89.16 + /- 16.17 versus 2.30 + /- 0.89 mU / mg protein , P & lt ; 0.01 by colorimetric assessment of Bz - Gly - Gly - Gly degradation ) . - pubmed
Topical application of histamine stimulated secretion of ACE activity into nasal lavage fluid ( 2.90 + /- 0.88 versus 1.53 + /- 0.45 U / liter after saline provocation , P & lt ; 0.05 by Bz - Gly - Gly - Gly assay ) . - pubmed
In both histamine and allergen challenges , ACE release correlated closely with that of the vascular proteins IgG and albumin . - pubmed
Methacholine , a stimulant of glandular secretions , failed to augment ACE levels above baseline . - pubmed
ACE - immunoreactive material was localized by the immunogold technique with silver enhancement to the glycocalyx , between epithelial cells , and to interstitial , extracellular sites in the superficial lamina propria , with the highest intensity of staining immediately beneath the basement membrane . - pubmed
ACE was identified by immunoblotting as a 150 kD band on SDS - PAGE. - pubmed
Mechanism of the acyl - carbon cleavage and related reactions catalyzed by multifunctional P-450s : studies on cytochrome P-450 ( 17 ) alpha . - pubmed
It is now well - known that conventional cytochrome P-450s catalyze hydroxylation reactions using an iron mono - oxygen species , the structure of which , as inferred from chemical model studies , may be drrepresented by the following canonical forms : FeV==O<-->(.+)FeIV==O<-->FeIV -- O ( . - pubmed
Certain multifunctional P-450s , notably those involved in steroid biosynthesis , catalyze , in addition to hydroxylation reactions , an acyl - carbon cleavage process in which the participation of an iron peroxide intermediate , FeIII -- OOH , has been suggested . - pubmed
1,3 - Dihydro-5 - phenyl-2H - pyrido [ 3,2 - e ]- 1,4 - diazepin-2 - ones ( 11 - 13 ) , in which the chlorophenyl ring of 7 - chloro-1,4 - benzodiazepin-2 - ones is replaced by a pyridyl ring , were synthesized and evaluated as anticonvulsants using subcutaneous pentylenetetrazole ( scPTZ ) and maximal electroshock ( MES ) induced seizure screening tests . - pubmed
A methyl substituent at the N-1 and/or C-3 position of the diazepin-2 - one ring system was not a determinant of scPTZ activity , but the 1,3 - dimethyl derivative ( 13 ) was more potent than the parent 1,3 - unsubstituted compound ( 11 ) in the MES screen . - pubmed
TAM resistance was not related to the development of estrogen receptors , decreased TAM uptake , or the increased expression of the mdr-1 gene . - pubmed
The purpose of this study was to analyze the effects of nordihydroguaiaretic acid , an inhibitor of the lipoxygenase pathway of arachidonic acid , on antibody - dependent cellular cytotoxicity . - pubmed
The inhibition produced by nordihydroguaiaretic acid could not be reversed by leukotriene B4 , a 5 - lipoxygenase product . - pubmed
We demonstrate that small heat shock proteins ( sHsp ) inhibit in vitro amyloid formation by the Alzheimer 's A beta ( 1 - 42 ) polypeptide as detected by a thioflavine T fluorescence assay and electron microscopy . - pubmed
Proteins of the ATF / CREB class of transcription factors stimulate gene expression of several cell growth - related genes through protein kinase A - related cAMP response elements . - pubmed
However , numerous examples of self - catalyzed protein rearrangements have recently been discovered , including protein splicing and autoproteolysis of hedgehog proteins , glycosylasparaginases and pyruvoyl enzyme precursors . - pubmed
An experiment was performed to evaluate the effects of somatotropin on plasma free amino acid , urea and insulin concentrations and rumen fermentation pattern and to assess their relationships . - pubmed
Exogenous rbST increased plasma insulin concentration and the insulin response to feeding , and decreased plasma urea and free essential and branched chain amino acid concentrations . - pubmed
AIM : To examine whether alpha - hederin ( He d ) modulates hepatic detoxifying systems as a means of hepatoprotection . - pubmed
RESULTS : He d increased liver glutathione ( GSH ) content ( 20 % ) , but had no effect on GSH peroxidase , GSH reductase , and GSH S - transferase . - pubmed
The activities of superoxide dismutase and quinone reductase were unaffected by He d treatment . - pubmed
At the high dose of He d , catalase activity was decreased by 20 % . - pubmed
Hepatic content of metallothionein was dramatically increased ( 50 - fold ) , along with elevations of hepatic Zn and Cu concentrations ( 25%-80 % ) . - pubmed
Neuropeptide receptors couple via G - proteins to two principal signaling pathways that elevate cAMP through adenylate cyclase ( AC ) or mobilize intracellular Ca ( 2 + ) through phospholipase C ( PLC)-stimulated inositol phosphate ( IP ) turnover and production of inositol 1,4,5 - trisphosphate ( IP ( 3 ) ) . - pubmed
We showed previously that high - affinity receptors for pituitary adenylate cyclase - activating polypeptide ( PACAP ) are present on chick ciliary ganglion neurons and that receptor occupation increases cAMP production , resulting in enhanced acetylcholine sensitivity . - pubmed
After we suppressed AC activity and cAMP production with 2 '- 5 ' dideoxyadenosine , however , PACAP no longer increased acetylcholine sensitivity but instead reduced it , suggesting that an AC - independent signal pathway activated by PACAP inhibits some nicotinic acetylcholine receptors ( AChRs ) . - pubmed
Without previous AC block , both the rapidly desensitizing , alpha - bungarotoxin ( alphaBgt)-sensitive alpha7 - AChRs and the slowly desensitizing , alphaBgt - insensitive alpha3 * - AChRs on the neurons were potentiated by PACAP . - pubmed
After AC blockade , however , PACAP inhibited alpha7 - AChRs but left alpha3 * - AChRs unaffected . - pubmed
The selective inhibition of alpha7 - AChRs appeared to use a PLC signaling pathway because it was not seen after lowering PLC activity or buffering intracellular Ca ( 2 + ) and was mimicked by dialyzing neurons with an IP ( 3 ) receptor agonist . - pubmed
PACAP also induced IP turnover and increased [ Ca ( 2 +)]( i ) assessed directly with Fluo-3AM imaging . - pubmed
In male brains , PGE2 - 9 - ketoreductase inhibitor decreased PGF2alpha release and increased that of PGE2 ; aromatase inhibitor decreased 17beta - oestradiol and increased testosterone release . - pubmed
In female brains , NO donor and PGE2 - 9 - ketoreductase inhibitor increased testosterone and PGE2 release and decreased that of 17beta - oestradiol and PGF2alpha ; NO synthase inhibitor decreased testosterone release and PGE2 and increased 17beta - oestradiol and PGF2alpha release ; PGF2alpha decreased testosterone release and increased 17beta - oestradiol release ; aromatase inhibitor decreased 17beta - oestradiol release and increased testosterone release . - pubmed
In female brains , NO donor and PGE2 - 9 - ketoreductase inhibitor decreased PGE2 - 9 - ketoreductase and aromatase activities ; PGF2alpha increased aromatase activity ; NO synthase inhibitor increased PGE2 - 9 - ketoreductase and aromatase activity . - pubmed
The data suggest that , in amplexing female brains , external and/or internal stimuli inhibit NO synthase , decreasing NO and activating PGE2 - 9 - ketoreductase ; in turn , PGF2alpha increases aromatase activity and 17beta - oestradiol release ; while , in amplexing male brains , stimuli inhibit aromatase activity , thereby increasing testosterone production . - pubmed
In particular , differences observed in subsites S(3 ) , S(4 ) and the loops involved in inhibitor interactions explain the preference of caspase-8 for substrates with the sequence ( Leu / Val )- Glu - X - Asp . - pubmed
We developed buffer conditions that suppressed dynamic instability behavior by approximately 10 - fold to minimize the contribution of dynamic instability to total tubulin - GTP exchange . - pubmed
This system was used to demonstrate that soxS expression is sufficient for the induction of resistance to the superoxide - generating drug paraquat and for the transcriptional activation of the sodA and micF genes . - pubmed
This selection yielded six independent chromosomal lacZ transcriptional fusions that were activated by either artificial expression of SoxS or exposure of wild - type cells to micromolar concentrations of paraquat . - pubmed
These systems consisted of a homologous series of n - alkanes as both analytes and mobile phases with a C18 reversed - phase adsorbent . - pubmed
Since the beta - spectrin phosphate sites are located near the C - terminal region and close to the head of the beta - chain that is involved in dimer - dimer interaction , we supposed that the beta - chain phosphorylation has an effect upon the interactions between spectrin dimers , ie the tetramerization process . - pubmed
Haemoglobin levels and body iron stores were measured monthly . - pubmed
OBJECTIVES : We investigated whether augmentation of bradykinin ( BK ) bioavailability with angiotensin - converting enzyme ( ACE ) inhibition is associated with reduced exercise - induced myocardial ischemia in hypertension . - pubmed
Moreover , no correlation was observed between the basal response to BK or the magnitude of its improvement with EN and with either the dose of EN or the improvement in exercise ischemic threshold . - pubmed
CD4(+ ) T lymphocytes participate in protective immunity to ehrlichial pathogens through production of gamma interferon ( IFN - gamma ) , which promotes switching to high - affinity immunoglobulin G ( IgG ) and activation of phagocytic cells to produce nitric oxide . - pubmed
This study was designed to determine the relative contributions of MSP1a and MSP1b proteins , which contain both variant and conserved amino acid sequences , in stimulating memory CD4(+ ) T - lymphocyte responses in calves immunized with native MSP1 . - pubmed
In the latter case , & gt ; or = 80 % of the PEN - induced apoptosis was dependent on the presence of wild - type p53 . - pubmed
Proinflammatory mediators , reactive oxygen species , and TGF - beta can activate pancreatic stellate cells and their synthesis of collagen I and III . - pubmed
Interestingly , T - cell responses to all three epitopes are restricted by the H-2K ( s ) molecule , and this skewed class I restriction may be associated with susceptibility to demyelinating disease . - pubmed
Bisphosphorylation of TnI reduces the affinity of the regulatory site on troponin C ( TnC ) for Ca ( 2 + ) by increasing the rate of Ca ( 2 + ) dissociation . - pubmed
The ability of phosphorylation of the mutant TnIs to affect Ca ( 2 + ) sensitivity has been assessed . - pubmed
We propose that TnI residues 16 - 29 bind to TnC stabilizing the " open " Ca ( 2 +)-bound state . - pubmed
Other neuromodulators that can influence intracellular Ca ( 2 + ) levels include the nucleoside adenosine , which acts via A ( 1 ) adenosine receptors subtypes ( A ( 1 ) ARs ) . - pubmed
Because A ( 1 ) AR activation inhibits glutamate release and action , A ( 1 ) AR activation may also contribute to neonatal brain injury . - pubmed
To examine this possibility , we treated primary neuronal cultures with the A ( 1 ) AR agonist CPA , the NMDAR antagonist MK801 , or CPA + MK801 . - pubmed
These observations show that A ( 1 ) AR activation and NMDAR blockade lead to early postnatal cell injury by mechanisms that involve inhibition of intracellular Ca ( 2 + ) signaling . - pubmed
We have studied three cell cycle markers in these models : 5 - bromo-2 '- deoxyuridine ( BrdU ) incorporation ( a marker of S phase ) , cdc2 protein expression ( a marker of G2 phase ) , and expression of MPM2 ( a marker of M phase ) , an epitope phosphorylated by cdc2 . - pubmed
We report here that postmitotic dopaminergic neurons undergoing PCD in the SNpc following 6 - OHDA and axotomy lesions incorporate BrdU and overexpress cdc2 , but do not express MPM2 . - pubmed
RESULTS : The liposome - plasmid DNA complexes affected metastatic capability in three different ways : TM - TPS : DOPE and DOTAP : DOPE had no effect on metastatic capability ; a suppressive effect was observed in DOSPA : DOPE and DMRIE : cholesterol ; while an augmentative effect was observed in DOTMA : DOPE and Effectene . - pubmed
This fact was showed by immunofluorescence analysis which confirmed that diosgenin strongly enhanced the activation of p53 in contrast to hecogenin and tigogenin actions . - pubmed
( 99m ) Tc - ECD SPET gave significantly higher uptake ratio values than ( 99m ) Tc - HMPAO SPET in several symmetrical clusters , including the right and left occipital cuneus , the left occipital and parietal precuneus , and the left superior and middle temporal gyri . - pubmed
( 99m ) Tc - HMPAO SPET gave significantly higher uptake ratio values than ECD in two smaller clusters , including the hippocampus in both hemispheres . - pubmed
In AD , relative brain uptake of ( 99m ) Tc - HMPAO and ( 99m ) Tc - ECD is different in several brain regions , some of which are typically involved in AD , such as the precuneus and the hippocampus . - pubmed
To analyze the relative sensitivity of the predicted hydrophobic face of the N - terminal heptad repeat to the insertion of uncharged , polar , and charged substitutions , mutations that introduced alanine , serine , or glutamic acid into positions 436 , 443 , 450 , and 457 of the envelope protein were examined . - pubmed
While no single amino acid change at any of the positions interfered significantly with the synthesis , processing , or transport to the plasma membrane of glycoprotein complexes , 9 of the 12 nonconservative mutations in these residues completely abolished fusion activity and virus infectivity . - pubmed
Mutations in the central positions ( 443 and 450 ) of the heptad repeat region were the most detrimental to Env function , and even single alanine substitutions in these positions dramatically altered the fusogenicity of the protein . - pubmed
When administered locally , the antihyperalgesic effects of morphine were mediated by peripheral opioid receptors in both males and females , since this effect was not reversed by i.c.v . - pubmed
The effect of gamma globulin and oral cortisone in the acute disease . - pubmed
MAIN OUTCOME MEASURES : The primary endpoint was reduction in urine N - telopeptides of type 1 collagen ( NTx ) corrected for creatinine level at 3 months . - pubmed
RESULTS : Over 3 months , ALN produced a significantly greater mean reduction in urine NTx than did RIS ( -52 % vs -32 % , p & lt ; 0.001 ) , which was maintained at 12 months . - pubmed
ALN produced a significantly greater mean BMD increase than did RIS at 6 months , and it was maintained at 12 months at the lumbar spine ( 4.8 % vs 2.8 % , p & lt ; 0.001 ) and total hip ( 2.7 % vs 0.9 % , p & lt ; 0.001 ) , as well as at the trochanter and femoral neck . - pubmed
Significant reductions in BSAP with ALN compared to RIS were maintained over the 12 months of treatment . - pubmed
Tolerability was generally similar between ALN , RIS and PBO , and the incidence of upper GI AEs causing discontinuation and oesophageal AEs was similar in the ALN and RIS groups . - pubmed
CONCLUSION : In this study , ALN 70 mg OW produced a 50 % greater reduction in bone resorption as measured by urine NTx and significantly greater increases in lumbar spine and hip BMD than did RIS 5 mg daily . - pubmed
Additional studies are needed comparing OW ALN with OW RIS , which became available after the commencement of the present study . - pubmed
Colchicine decreased the accumulated extracellular matrix and the multiple fibrotic nodules formed in cirrhotic liver , and eliminated alpha - smooth muscle actin ( alpha - SMA)-positive cells . - pubmed
In activated stellate cells , colchicine inhibited alpha - SMA and transforming growth factor - beta1 ( TGFbeta1 ) expression . - pubmed
Aminoacyl - tRNA ( aa - tRNA ) formation , an essential process in protein biosynthesis , is generally achieved by direct attachment of an amino acid to tRNA by the aa - tRNA synthetases . - pubmed
An exception is Gln - tRNA synthesis , which in eukaryotes is catalyzed by glutaminyl - tRNA synthetase ( GlnRS ) , while most bacteria , archaea , and chloroplasts employ the transamidation pathway , in which a tRNA - dependent glutamate modification generates Gln - tRNA . - pubmed
Early work suggested that mitochondria use the transamidation pathway for Gln - tRNA formation . - pubmed
Serum calcium , phosphate , parathyroid hormone , 1,25 - dihydroxyvitamin D , bone alkaline phosphatase ( BAP ) , osteocalcin , and urine calcium were measured . - pubmed
RESULTS : Alendronate caused significant increase in serum osteocalcin and BAP levels biochemically and the number of osteoblasts histopathologically . - pubmed
Deletion of orfR , a gene immediately upstream of orf1 - orf2 - omcB that encodes a putative transcriptional regulator , significantly lowered the omcB transcription when Fe ( III ) was the electron acceptor and partially inhibited Fe ( III ) reduction . - pubmed
From P8 to P14 , there was a marked increase in doublecortin immunoreactive cells in the AMPA - injected SVZ . - pubmed
Blockade of the renin - angiotensin - aldosterone - system ( RAAS ) attenuates hepatic fibrosis . - pubmed
Serum laminin ( LN ) and hyaluronic acid ( HA ) were measured using radioimmunoassays . - pubmed
Perindopril treatment significantly reduced mean fibrosis score , protein levels of AT1R , TGF - beta1 and PDGF - BB , serum levels of HA and LN , and the activity of MMP-2,9 . - pubmed
NF - kappaB DNA binding activity markedly increased in model group , perindopril treatment considerably reduced NF - kappaB DNA binding activity . - pubmed
FAD oxidizes the ERO1 - PDI electron transfer chain : the role of membrane integrity . - pubmed
Microsomal membrane integrity was mandatory for all FAD - related oxidation steps downstream of Ero1 . - pubmed
The PDI inhibitor bacitracin could inhibit FAD - mediated oxidation of microsomal proteins and PDI , but did not hinder the FAD - driven oxidation of Ero1 . - pubmed
Our data demonstrated that Ero1 can utilize FAD as an electron acceptor and that FAD - driven protein oxidation goes through the Ero1 - PDI pathway and requires the integrity of the endoplasmic reticulum membrane . - pubmed
Our findings prompt further studies to elucidate the membrane - dependent steps of PDI oxidation and the role of FAD in redox folding . - pubmed
The significantly improved therapeutic outcome of animals receiving the triple therapy was attributed to multiple factors , including most likely improved FMG expression throughout the tumor and enhanced sensitivity of the tumor cells to gemcitabine by adenoviral gene products but also FMG expression . - pubmed
HscA and HscB stimulate [ 2Fe-2S ] cluster transfer from IscU to apoferredoxin in an ATP - dependent reaction . - pubmed
CD spectra indicate that both HscB and HscA interact with [ 2Fe-2S]IscU and the rate of cluster transfer was stimulated more than 20 - fold in the presence stoichiometric HscA and HscB and excess MgATP . - pubmed
No stimulation was observed in the absence of either HscB or MgATP , and cluster transfer was found to be an ATP - dependent reaction based on concomitant phosphate production and the enhanced rates of cluster transfer in the presence of KCl which is known to stimulate HscA ATPase activity . - pubmed
The results demonstrate a role of the ISC HscA / HscB cochaperone system in facilitating efficient [ 2Fe-2S ] cluster transfer from the IscU scaffold protein to acceptor proteins and that [ 2Fe-2S ] cluster transfer from IscU is an ATP - dependent process . - pubmed
279 , 53924 - 53931 ] , and mechanistic proposals for coupling of the HscA ATPase cycle with cluster transfer from [ 2Fe-2S]IscU to apo - IscFdx are discussed . - pubmed
Surprisingly , modifications in sustained K (+ ) currents in eag and Shab mutants increased thresholds for DD induction and GF pathway failure . - pubmed
The search for target analytes to uncover the misuse of long acting insulin analogues ( Lantus , Insulin Glargine ; Levemir , Insulin Detemir ) in doping control samples led to the identification of several degradation products of insulin or its synthetic analogues . - pubmed
In this study , we evaluated in vitro and in vivo the effects of ZD4054 , an orally active antagonist that specifically binds ET ( A ) R , as monotherapy , and in combination with paclitaxel . - pubmed
ZD4054 treatment also resulted in a reduction of ET ( A ) R - driven angiogenesis and invasive mediators , such as vascular endothelial growth factor , cyclooxygenase-1 / 2 , and matrix metalloproteinase ( MMP ) . - pubmed
The combination of ZD4054 and paclitaxel led to the potentiation of all these effects , indicating that ZD4054 , by blocking the ET ( A ) R - dependent proliferative , invasive , and antiapoptotic signals , can enhance sensitivity to paclitaxel . - pubmed
Furthermore , ZD4054 - dependent tumor growth inhibition was associated with a reduction in proliferation index , microvessel density , and MMP-2 expression . - pubmed
Ouabain , a potent inhibitor of the Na +- K + pump has been shown to reduce fluid absorption in animal models of PD . - pubmed
The results of the present study , showing a rapid effect of ouabain on the RTM of visceral peritoneum , therefore clearly suggest that cell membrane Na + K +- ATPase is important for peritoneal ionic transport . - pubmed
Water washed manganese nodule leached residue ( WMNLR ) calcined at different temperatures was characterized by XRD , FTIR , TG - DTA , surface area , surface oxygen , and surface acid sites . - pubmed
He was transferred to our center for F-18 FDG PET / CT scan under the suspicion of occult malignancy . - pubmed
The PET / CT scan showed increased FDG uptake in the right cervical lymphadenopathy and multiple bony lesions . - pubmed
After another 6 months of adjusted antituberculosis therapy with isoniazid , rifampin , ethambutol , and clarithromycin , a repeat PET / CT scan showed remission of previous lesions . - pubmed
PURPOSE : The role of arginine vasopressin in heart failure and the use of vasopressin receptor antagonists in the treatment of heart failure are reviewed . - pubmed
Folding thermodynamics and kinetics of tRNA(Phe ) , pseudoknots , and mRNA fragments in DMD simulations are in agreement with previous experimental findings . - pubmed
( 2 ) The percentage of CD3- CD56 + Nk cells in group A ( 44 + /- 5)% and in group B ( 48 + /- 4)% were significantly higher when compared to the control group ( 35 + /- 3)% ( P & lt ; 0.05 ) , there was no significantly difference between the two mifepristone - treated groups ( P & gt ; 0.05 ) ; The percentage of CD3- CD56 + CD16- subset in group A ( 42 + /- 5)% and in group B ( 45 + /- 5)% were significantly higher when compared to the control group ( 33 + /- 3)% , ( P & lt ; 0.05 ) , there was no significantly difference between the two mifepristone - treated groups ( P & gt ; 0.05 ) ; the percentages of CD3- CD56 + CD16 + subset in three groups [ ( 2.70 + /- 0.24)% ( 3.26 + /- 0.37)% and ( 2.33 + /- 0.29)% ] were not significantly different ( P & gt ; 0.05 ) . - pubmed
BACKGROUND : Magnesium ( Mg ) is an essential cofactor for enzymes involved in glucose and insulin metabolism . - pubmed
The MS was defined based on criteria set by the Third Report of the National Cholesterol Education Program except that body mass index was used in place of waist circumference . - pubmed
Logistic regression analysis was used to examine the association between quartile categories of Mg intake , prevalence of MS and components of the MS . - pubmed
RESULTS : Mg intake was inversely associated with the MS ; those with the highest intake of Mg had significantly lower risk of having MS compared to the lowest quartile of intake ( OR : 0.36 , 95 % CI 0.19 - 0.69 , P for trend 0.002 ) . - pubmed
Glutathione regulates integrin alpha ( IIb ) beta(3)-mediated cell adhesion under flow conditions . - pubmed
Experiments conducted under static conditions using platelet - rich plasma show that platelet activation and adhesion activity of alpha ( IIb ) beta ( 3 ) are regulated by glutathione ( GSH - GSSG ) redox potential . - pubmed
However , it remains unclear as to whether GSH - GSSG exerts its regulatory role in platelets by direct targeting of alpha ( IIb ) beta ( 3 ) or intracellular signals that activate the integrin . - pubmed
GSH - GSSG dose - dependently reduced the number of adherent cells to fibrinogen and VWF under 2.5 dyn / cm(2 ) of shear stress , a physical force calculated to be 110 dyne on platelets . - pubmed
GSH treatment also abolished the hyper - adhesion activity of cells expressing the Pro33 variant of alpha ( IIb ) beta ( 3 ) . - pubmed
The results suggest that GSH may have distinct effects on agonist - induced alpha ( IIb ) beta ( 3 ) activation and on the alpha ( IIb ) beta ( 3 )- fibrinogen or alpha ( IIb ) beta ( 3 )- VWF bonds when exposed to fluid shear stress . - pubmed
Interaction of carbon monoxide with the apoptosis - inducing cytochrome c - cardiolipin complex . - pubmed
The interaction of mitochondrial cytochrome ( cyt ) c with cardiolipin ( CL ) is involved in the initial stages of apoptosis . - pubmed
This interaction can lead to destabilization of the heme - Met80 bond and peroxidase activity [ Basova , L. V. , et al . - pubmed
We show that under these conditions carbon monoxide ( CO ) binds to cyt c , with very high affinity ( approximately 5 x 10(7 ) M(-1 ) ) , in contrast to the native cyt c protein involved in respiratory electron shuttling that does not bind CO . - pubmed
Binding of CO to the cyt c - CL complex inhibits its peroxidase activity . - pubmed
Photodissociated CO from the cyt c - CL complex shows & lt;20 % picosecond geminate rebinding and predominantly bimolecular rebinding , with a second - order rate constant of approximately 10(7 ) M(-1 ) s(-1 ) , an order of magnitude higher than in myoglobin . - pubmed
Together with the findings that ( a ) approximately 30 CL per cyt c are required for full CO binding and ( b ) salt - induced dissociation indicates that the two negative headgroup charges interact with approximately 5 positive surface charges of the protein , these results are consistent with a CL anchorage model with an acyl chain impaled in the protein [ Kalanxhi , E. , and Wallace , C. J. - pubmed
The affinity of CO for the complex is high enough to envisage an antiapoptotic effect of nanomolar CO concentrations via inhibition of the cyt c peroxidase activity . - pubmed
The high - affinity and selective sigma ( 1 ) antagonists BD-1063 , BD-1047 and NE-100 , administered subcutaneously ( s.c . ) - pubmed
The effects of the sigma ( 1 ) antagonists were reversed by the sigma ( 1 ) agonist PRE-084 ( 32 mg / kg , s.c . ) - pubmed
These results suggest that sigma ( 1 ) receptors are essential for capsaicin - induced mechanical hypersensitivity , but are not involved in mechanical nociceptive pain . - pubmed
Compound C caused G(2)/M cell cycle block , accompanied by apoptotic glioma cell death characterized by caspase activation , phosphatidylserine exposure and DNA fragmentation . - pubmed
RESULTS : Among these GEM sensitivity - related genes , dCK alone showed a significant correlation with GEM efficacy . - pubmed
CONCLUSION : dCK mRNA expression is a candidate indicator for GEM efficacy in unresectable pancreatic cancer . - pubmed
The purpose of this study is to identify the hierarchy of importance amongst pathways involved in fatty acid ( FA ) metabolism and their regulators in the control of hepatic FA composition . - pubmed
The resulting model included FA uptake by the liver , FA oxidation , elongation and desaturation of FA , which were found active in both genotypes during fasting . - pubmed
The models showed that FA were oxidized at a constant and small rate , whereas desaturation of FA also occurred during fasting . - pubmed
RESULTS : The swiprosin-1 augmented PMA / A23187 - induced expression of cytokines and release of histamine . - pubmed
However , knock - down of swiprosin-1 showed only a modest effect on PMA / A23187 - induced cytokine expression , suggesting that swiprosin-1 has gain - of - function characteristics . - pubmed
Importantly , either disruption of actin by cytochalasin B or inhibition of PI3 kinase , an enzyme involved in actin remodeling , by wortmannin blocked cytokine expression only in swiprosin-1 - overexpressing cells . - pubmed
The body mass index , weight , waist circumference , fat mass , blood pressure , total cholesterol , and low - density lipoprotein cholesterol had decreased significantly . - pubmed
The correlation analysis showed a positive association between the baseline visfatin and total cholesterol level ( r = 0.49 ; P & lt;.01 ) . - pubmed
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single - chain variable - fragment ( scFv ) as a recognition element . - pubmed
The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on the pharmacophore of fluoroquinolone antibiotics and the subsequent selection of scFv receptor modules against these antibiotics - based antigens using phage display . - pubmed
A2 is a specific binder for enrofloxacin and did not cross - react with other fluoroquinolone antibiotics including structurally similar ciprofloxacin , while F9 is a general fluoroquinolone binder that likely bound to the antigen at the common pyridone - carboxylic acid pharmacophore . - pubmed
These scFv - based receptors were successfully applied to the development of one - step fluorescent biosensor which can detect fluoroquinolone antibiotics at concentrations below the level suggested in animal drug application guidelines . - pubmed
The present results verify that in the beta-(1 4)-linkage intramolecular hydrogen bonding in the aqueous phase is due to the decreased ability of water to successfully compete for the O5 ' and HO3 hydrogen bonding moieties , in contrast to that occurring between the O5 ' and HO6 atoms in this alpha-(1 4)-linkage . - pubmed
Phosphatidylinositol 4 - kinases ( PI4Ks ) regulate vesicle - mediated export from the Golgi apparatus via phosphatidylinositol 4 - phosphate ( PtdIns4P ) binding effector proteins that control vesicle budding reactions and regulate membrane dynamics . - pubmed
An important hub of this network is protein kinase D , which regulates the activity of PI4 K and several PtdIns4P effectors that control sphingolipid and sterol content of Golgi membranes . - pubmed
Other newly identified PtdIns4P effectors include Vps74 / GOLPH3 , a phospholipid flippase called Drs2 and Sec2 , a Rab guanine nucleotide exchange factor ( GEF ) . - pubmed
IPC - induced increase in decoy receptor expression was reduced by prior treatment with CAPE , a nuclear factor - kappa B inhibitor ( NFkappaB ) . - pubmed
( rac )- 1,1 '- Binaphthyl - based simple receptors 1 and 2 have been designed , synthesized and studied theoretically . - pubmed
The receptors utilize naphthyridine as the binding motifs for complexation of dicarboxylic acids in CHCl ( 3 ) . - pubmed
The emission of the BINOL moiety was monitored experimentally to ascertain the selectivity and sensitivity of the receptors . - pubmed
Receptor 1 distinguishes maleic acid from isomeric fumaric acid by exhibiting different fluorescence behavior and demonstrates stronger binding in the excited state . - pubmed
The atomistic calculations provide insight into the differential dicarboxylic acid binding behavior of receptors 1 and 2 . - pubmed
MRP1 co - transports its substrate with glutathione ( GSH ) , leading to lower intracellular GSH . - pubmed
In this report we tested whether depleting intracellular GSH in MRP1 - expressing cells could hyper - sensitize them to anticancer drugs or not . - pubmed
We have found that the GSH contents in MRP1 - expressing cells are significantly lower than their corresponding control cells . - pubmed
The treatment with MRP1 substrate verapamil or the GSH synthetase inhibitor buthionine sulfoxi - mine significantly reduced the intracellular GSH contents in MRP1 - expressing cells . - pubmed
Vitamin D receptor gene polymorphisms and HLA DRB1 * 04 cosegregation in Saudi type 2 diabetes patients . - pubmed
The vitamin D receptor ( VDR ) gene has been involved in the modulation of susceptibility to inflammatory and autoimmune conditions , and could play a role in the pathogenesis of type 2 diabetes mellitus ( T2DM ) . - pubmed
Genomic DNA was genotyped for the VDR gene single nucleotide polymorphisms of Fok-1 , Taq-1 , ApaI , and Bsm - I. - pubmed
Notably , the VDR polymorphisms observed to be more frequent in T2DM patients correlated with increased VDR expression and IL-12 production , as well as with metabolic parameters of susceptibility to T2DM , including serum cholesterol and high - density lipoprotein levels . - pubmed
The overall prevalence of MetS was 19.0 % ( 16/84 ; median treatment duration = 14 months ) in SGA - treated and 0.8 % ( 1/127 ) in SGA - naive children ( OR 29.7 ; 95 % CI 3.85 to 228.40 , P & lt ; 0.001 ) , with an increased prevalence of all components except high - density lipoprotein cholesterol ( HDL - C ) , respectively : elevated WC ( 40.7 % and 10.1 % ; P & lt ; 0.001 ) ; hypertriglyceridemia ( 33.7 % and 18.8 % ; P = 0.01 ) ; impaired fasting glucose ( 12.5 % and 0.7 % ; P = 0.005 ) ; and elevated blood pressure ( 41.2 % and 16.5 % ; P & lt ; 0.001 ) . - pubmed
Presence of abdominal obesity was more sensitive ( 92.9 % ) than BMI ( 68.8 % ) , while fasting glucose of 5.6 mmol / L or more and HDL - C of 1.03 mmol / L or less were most specific ( 94.1 % ) in correctly identifying MetS. - pubmed
Hydrogen sulfide decreases the levels of ROS by inhibiting mitochondrial complex IV and increasing SOD activities in cardiomyocytes under ischemia / reperfusion . - pubmed
We found that H ( 2 ) S inhibited mitochondrial complex IV activity and increased the activities of superoxide dismutases ( SODs ) , including Mn - SOD and CuZn - SOD . - pubmed
Further studies indicated that H ( 2 ) S up - regulated the expression of Mn - SOD but not CuZn - SOD . - pubmed
Using a cell - free system , we showed that H ( 2 ) S activates CuZn - SOD . - pubmed
An isothermal titration calorimetry ( ITC ) analysis indicated that H ( 2 ) S directly interacts with CuZn - SOD . - pubmed
Taken together , H ( 2 ) S inhibits mitochondrial complex IV and activates SOD to decrease the levels of ROS in cardiomyocytes during I / R. - pubmed
AIM : To predict simultaneously the area under the concentration - time curve during one dosing interval [ AUC(0,12 h ) ] for mycophenolic acid ( MPA ) and tacrolimus ( TAC ) , when concomitantly used during the first month after transplantation , based on common blood samples . - pubmed
EXPERT OPINION : Mechanistic studies have discovered that TSIIA modulates ion channels , kinases , hormone receptors , apoptosis proteins , growth factors , cytokines , microRNA , tumor suppressor and many other targets , revealing an intricate biological network modulated by TSIIA . - pubmed
GC - MS analysis of circulating methyl farnesoids during the mid to late 3rd instar showed that methyl farnesoate is predominant over methyl epoxyfarnesoate (= JH III ) . - pubmed
An equilibrium binding assay confirmed that the mutation ( Q288A ) strongly altered methyl farnesoate interaction with USP , while a heterologous cell - line transfection assay confirmed that the mutation did not allosterically alter the transcriptional response of the ultraspiracle / ecdysone receptor heterodimer to ecdysteroid signaling . - pubmed
These results suggest that normal larval to pupal maturation in this mecopteran model insect requires the involvement of a distinct endocrine axis of USP binding to its own endogenous terpenoid ligand . - pubmed
Ceramide 1 - phosphate mediates endothelial cell invasion via the annexin a2 - p11 heterotetrameric protein complex . - pubmed
We have now identified and characterized interactions between ceramide 1 - phosphate and the annexin a2 - p11 heterotetramer constituents . - pubmed
Although the annexin a2 - p11 heterotetramer constituents do not bind the lipid C-1 - P exclusively , other structurally similar lipids , such as phosphatidylserine , sphingosine 1 - phosphate , and phosphatidic acid , could not elicit the potent chemotactic stimulation observed with C-1 - P. - pubmed
These results imply that extracellular C-1 - P , acting through the extracellular annexin a2 - p11 heterotetrameric protein , can mediate vascular endothelial cell invasion . - pubmed
Downregulation of miR-133 via MAPK / ERK signaling pathway involved in nicotine - induced cardiomyocyte apoptosis . - pubmed
Consistently , caspase-9 , a target gene for miR-133 , was significantly upregulated , leading to an increase in caspase-3 , in nicotine - treated cardiomyocytes compared to non - treated cells . - pubmed
Lipid peroxidation ( LPO ) and reduced glutathione ( GSH ) were measured by standard methods . - pubmed
It was also observed that there was a significant correlation between SIBO with IL-6 , IL-8 , TNF - alpha , and IL-10 , LPO and GSH . - pubmed
In stefin B - deficient bone marrow - derived macrophages ( BMDMs ) , we detected an increase in the induction of the LPS - induced pro - inflammatory signal nitric oxide ( NO ) but decreased IL-10 expression . - pubmed
Most importantly , lung cancer cells themselves upregulated IL-6 secretion by activating the p38 / NF - kappaB pathway through treatment with cisplatin and camptothecin . - pubmed
Juvenile stress reduced the maximum amplitude of the adrenocorticotropic hormone ( ACTH ) levels ( reduced peak at lights off ) , without affecting the circadian corticosterone rhythm , but other aspects of the HPA function ( negative glucocorticoid feedback , responsiveness to further stressors and brain gene expression of corticotrophin - releasing hormone and corticosteroid receptors ) remained unaltered . - pubmed
coli strains carrying mutations in PmrAB that confer resistance to polymyxins , which might have evolved in vivo and have been rarely detected , are described for the first time in enterobacteria isolated from animals . - pubmed
MLD was then performed on the patterns to deposit organic films predominantly on the Si surface , with only small amounts growing on the Cu regions . - pubmed
A negative potential bias was subsequently applied to the pattern to selectively desorb the layer of SAMs electrochemically from the Cu surface while preserving the MLD films on Si . - pubmed
P74308 is the most efficient enzyme for 4 - ONE discovered until now . - pubmed
The role of the little investigated carbonyl - reducing enzymes in detoxification seems to be in fact a very old process with rarely observed nonhyperbolic enzyme kinetics as an adaptation mechanism to higher concentrations of reactive oxygen species . - pubmed
Licochalcone A up - regulates of FasL in mesenchymal stem cells to strengthen bone formation and increase bone mass . - pubmed
Here we found that 0.35 mg / L Licochalcone A ( L - A ) had a strong effect in increasing the osteogenic differentiation and mineralization of BMSCs both in vivo and in vitro by up - regulating FasL and further playing a role in regulating the ERK and GSK-3beta - catenin systems . - pubmed
Results of our study suggested that careful titration of MSC was response to L - A and up - regulated FasL pathways mediating differentiation of ERK and GSK-3beta - catenin biological systems under disease state in vivo , restore the impaired function , is one of the ways of L - A relieve or treatment osteoporosis . - pubmed
SHP represents a highly conserved ancient PTP , and studying MbSHP should provide a better understanding about the evolution of tyrosine phosphorylation . - pubmed
Indeed , Delta13Met expression led to a decrease in HGF - induced tyrosine phosphorylation of Met and ERK phosphorylation , as well as cell proliferation and migration via sequestration of HGF . - pubmed
At 6 weeks post - ACLR , GHK - Cu groups resulted in a smaller side - to - side difference in knee laxity as compared to the saline group ( p = 0.009 ) , but there was no significant difference at 12 weeks post - operation . - pubmed
The graft complex in the 0.3 mg / ml GHK - Cu group had higher stiffness than saline group at 6 weeks post - operation ( p = 0.026 ) , but there was no significant difference in ultimate load , gait parameters , and histological scores among treatment groups . - pubmed
Body and testis weights , serum testosterone , testis malondialdehyde ( MDA ) , catalase ( CAT ) , superoxide dismutase ( SOD ) , glutathione ( GSH ) , glutathione S transferase ( GST ) and protein levels were measured , and testis tissue was examined histopathologically and immunohistochemically ( PCNA ) . - pubmed
MRME treatment rendered significant increases in body weight , testis weight , testes - body weight ratio , down regulated the MDA levels , reduced the degeneration and disruption of seminiferous tubule structure , restored the antioxidant enzymes and serum testosterone levels , increased the PCNA activities and attenuated the testes injury . - pubmed
A novel oral delivery system that TPGS modified docetaxel proniosomes , DTX - TPGS - PN , was developed and the characterization after hydration was observed . - pubmed
Firstly , Doce - TPGS - PN was optimized by investing the factors , including the type of surfactant , methods of adding TPGS , content of TPGS and the molar ratio of span40 / cholesterol , which may affecting the particle size , encapsulation efficiency and instantaneous release of drug in the formulation . - pubmed
These results indicate the oral delivery system of DTX - TPGS - PN was successfully built with good properties . - pubmed
Furthermore , albumin binds metals and heme thereby limiting their ability to produce reactive oxygen and reactive nitrogen species . - pubmed
Similar to pathogenic processes that occur during epilepsy , extravasated albumin could induce the expression of proinflammatory cytokines and affect the ability of astrocytes to maintain potassium homeostasis thereby possibly making neurons more vulnerable to glutamate exicitotoxicity , which is thought to be a pathogenic mechanism in MS . - pubmed
Hypoacetylation of the cells in the presence of acetyltransferase inhibitor , garcinol generally reduced efficiency of BER of 8 - oxoG damage , indicating that some steps in the pathway are stimulated by acetylation . - pubmed
BACKGROUND : The fibrotic capsule that surrounds silicone implants consists mainly of collagen . - pubmed
( 4 ) The protein expressions of TGF - beta1 of cells in group BC and 1 , 3 mumol / L SD-208 groups were respectively 1.00 + -0.08 , 0.80 + -0.08 , and 0.61 + -0.05 , and the protein expressions of TGF - beta1 of cells in 1 , 3 mumol / L SD-208 groups were significantly lower than those in group BC ( with t values respectively 4.0 and 9.2 , P values below 0.01 ) . - pubmed
We quantified urinary 2,4 - D using LC / MS / MS . - pubmed
CD62L test at 2 years of natalizumab predicts progressive multifocal leukoencephalopathy . - pubmed
Exposure to Cr ( VI ) significantly increased MDA contents and organ coefficient , whereas decreased T - SOD activities , Ca2 + - ATPase activities , mitochondrial membrane potential and GSH contents , and histological studies demonstrated renal damage . - pubmed
RESULTS : The ethyl acetate fraction at 200 mg / kg produced significant ( p<0.05 ) elevations of catalase enzyme activity and a significant ( p<0.05 ) reduction in serum malondialdehyde . - pubmed
Furthermore , 17beta - estradiol downregulated the expression of intercellular adhesion molecule-1 ( P = .0001 ) and P - selectin ( P & lt ; .0001 ) on the endothelium and increased the protein expression of eNOS ( P & lt ; .0001 ) . - pubmed
CONCLUSIONS : The prophylactic treatment with 17beta - estradiol showed better overall repercussions and was able to prevent any fatal occurrence , increase eNOS expression , thus preserving mesenteric perfusion and intestinal integrity , and reduce inflammation . - pubmed
Exposing SCLC lines to tubulin - targeted agents ( paclitaxel or vinorelbine ) simultaneously with etoposide / carboplatin resulted primarily in less than additive cell killing . - pubmed
As single agents , nuclear kinase inhibitors including Aurora kinase inhibitors , Kinesin Spindle Protein / EG5 inhibitors , and Polo - like kinase-1 inhibitors were potent cytotoxic agents in SCLC lines ; however , simultaneous exposure of the SCLC lines to these agents along with etoposide / carboplatin , generally , resulted in less than additive cell killing . - pubmed
Exposure of the SCLC lines to the MDM2 inhibitor JNJ-27291199 produced enhanced killing in 80 % of the SCLC lines . - pubmed
Chk-1 inhibitors such as rabusertib increased the cytotoxicity of etoposide / carboplatin to the SCLC lines in an additive to greater than additive manner . - pubmed
The combination of GSK-3beta inhibitor LY-2090314 with etoposide / carboplatin increased killing in approximately 40 % of the SCLC lines . - pubmed
Exposure to the BET bromodomain inhibitor MK-8628 increased the SCLC cell killing by etoposide / carboplatin in 20 - 25 % of the SCLC lines . - pubmed
Only 10 - 15 % of the SCLC lines had an increased response to etoposide / carboplatin when simultaneously exposed to the PARP inhibitor talazoparib . - pubmed
Efficacy research of salazosulfamide in ankylosing spondylitis and NAT1 gene polymorphism . - pubmed
The aim of this study was to explore the correlation of salazosulfamide efficacy on ankylosing spondylitis and N - acetyltransferase 1 ( NAT1 ) gene polymorphism . - pubmed
Overall , salazosulfamide demonstrates curative activity for ankylosing spondylitis and we believe that NAT1 AA / GG genotype at 263 locus can promote salazosulfamide effectiveness on ankylosing spondylitis . - pubmed
Serum parathyroid hormone and alkaline phosphatase as predictors of calcium requirements after total parathyroidectomy for hypocalcemia in secondary hyperparathyroidism . - pubmed
RESULTS : There is a significant correlation between the postoperative amount of calcium requirement and preoperative serum alkaline phosphatase level ( r = 0.442 ; P & lt ; .001 ) and parathyroid hormone level ( PTH ; r = 0.889 ; P & lt ; .001 ) . - pubmed
For prediction of insufficient calcium supplement , the cutoff point of PTH and alkaline phosphatase level values were calculated by receiver operating characteristic ( ROC ) curve analysis and modified equations were developed by regression analysis . - pubmed
Therefore , we investigated whether concomitant inhibition of the two main antioxidant defense pathways , that is , the thioredoxin ( TRX ) and the glutathione ( GSH ) systems , presents a new strategy to trigger cell death in RMS . - pubmed
gamma - glutamylcysteine synthetase inhibitor , buthionine sulfoximine ( BSO ) or the cystine / glutamate antiporter inhibitor erastin ( ERA ) , synergize with thioredoxin reductase ( TrxR ) inhibitor auranofin ( AUR ) to induce cell death in RMS cells . - pubmed
Interestingly , AUR causes accumulation of ubiquitinated proteins when combined with BSO or ERA , in line with recent reports showing that AUR inhibits the proteasome besides TrxR. - pubmed
Consistently , AUR / BSO or AUR / ERA cotreatment increases ubiquitination and expression of the short - lived proteins NOXA and MCL-1 , accompanied by increased binding of NOXA to MCL-1 . - pubmed
Notably , NOXA knockdown significantly rescues RMS cells from AUR / BSO- or AUR / ERA - induced cell death . - pubmed
In addition , AUR acts together with BSO or ERA to stimulate BAX / BAK and caspase activation . - pubmed
Importantly , re - supply of GSH or its precursor NAC completely prevents AUR / ERA- and AUR / BSO - induced accumulation of ubiquitinated proteins , NOXA upregulation and cell death , indicating that GSH depletion rather than ROS production is critical for AUR / BSO- or AUR / ERA - mediated cell death . - pubmed
M344 promotes nonamyloidogenic amyloid precursor protein processing while normalizing Alzheimer ' s disease genes and improving memory . - pubmed
We show that the small - molecule histone deacetylase inhibitor M344 reduces beta - amyloid ( Abeta ) , reduces tau Ser396 phosphorylation , and decreases both beta - secretase ( BACE ) and APOEepsilon4 gene expression . - pubmed
M344 increases the expression of AD - relevant genes : BDNF , alpha - secretase ( ADAM10 ) , MINT2 , FE65 , REST , SIRT1 , BIN1 , and ABCA7 , among others . - pubmed
M344 increases sAPPalpha and CTFalpha APP metabolite production , both cleavage products of ADAM10 , concordant with increased ADAM10 gene expression . - pubmed
M344 also increases levels of immature APP , supporting an effect on APP trafficking , concurrent with the observed increase in MINT2 and FE65 , both shown to increase immature APP in the early secretory pathway . - pubmed
The roles of RNA 5 - methylcytosine ( RNA : m5C ) and RNA : m5C methyltransferases ( RCMTs ) in lineage - associated chromatin organization and drug response / resistance are unclear . - pubmed
hnRNPK interacts with the lineage - determining transcription factors ( TFs ) , GATA1 and SPI1 / PU.1 , and with CDK9 / P - TEFb to recruit RNA - polymerase - II at nascent RNA , leading to formation of 5 - Azacitidine ( 5 - AZA)-sensitive chromatin structure . - pubmed
In contrast , NSUN1 binds BRD4 and RNA - polymerase - II to form an active chromatin structure that is insensitive to 5 - AZA , but hypersensitive to the BRD4 inhibitor JQ1 and to the downregulation of NSUN1 by siRNAs . - pubmed
This study reveals novel RNA : m5C / RCMT - mediated chromatin structures that modulate 5 - AZA response / resistance in leukaemia cells , and hence provides a new insight into treatment of leukaemia . - pubmed
By probing ( Mo2/3 M1/32)2 AC ( where M2 = Sc , Y and A = Al , Ga , In , Si , Ge , In ) , we predict seven stable i - MAX phases , five of which should have a retained stability at high temperatures . - pubmed
Furthermore , based on i - MAX phases as parent materials for 2D MXenes , we also expect that the range of MXene compositions will be expanded . - pubmed
RESULTS : CCl4 administration increased liver weight , decreased body weight , elevated plasma alanine aminotransferase , and aspartate aminotransferase and increased liver oxidative stress ( malondialdehyde and glutathione ) . - pubmed
Moreover , SE inhibited CCl4 - induced nuclear translocation of hepatic nuclear factor kappa B ( NF - kappaB ) p65 and phosphorylation of mitogen - activated protein kinase ( MAPK ) . - pubmed
CONCLUSION : These results suggested that SE prevented CCl4 - induced hepatic fibrosis by inhibiting the MAPK and NF - kappaB signaling pathways . - pubmed
Formaldehyde ( FA ) is a ubiquitous environmental pollutant , which can induce apoptosis in lung cell and is related to the pathogenesis of asthma , pneumonia , and chronic obstructive pulmonary disease . - pubmed
Heat shock protein 70 ( Hsp70 ) is an ATP - dependent molecular chaperone and exhibits an anti - apoptosis ability in a variety of cells . - pubmed
Inhibitor ( LY 294002 , a specific inhibitor of PI3K - Akt ) test result indicated that PI3K - Akt signaling pathway was involved in the inhibition of FA - induced apoptosis by Hsp70 overexpression and also active in the maintenance of GLI2 level . - pubmed
The influence of different ratios of starch / polyvinyl alcohol ( PVA)/citric acid and different drying times on the performance properties , transparency , tensile strength ( TS ) , water vapor permeability ( WVP ) , water solubility ( WS ) , color difference ( DeltaE ) , and antimicrobial activity of the ternary blends films were investigated . - pubmed
We found that niclosamide - induced endosomal neutralization prevented E glycoprotein conformational changes on the virion surface of flaviviruses , resulting in the release of non - infectious immature virus particles with uncleaved pr peptide from host cells . - pubmed
Kidney tissues were harvested at 8 weeks post - STZ and analyzed by a hydrophilic interaction liquid chromatography ( HILIC ) ultra - performance liquid chromatography - quadrupole time - of flight mass spectrometry ( UPLC - QTOF MS ) . - pubmed
Meprin expression - associated metabolites with strong variable importance of projection ( VIP ) scores were indoxyl sulfate , Ng - L - Glutamyl - L - aspartic acid , N - Methyl-4 - pyridone-3 - carboxamide , inosine , and cis-5 - decenedioic acid . - pubmed
Meprin deficiency - associated differentiating metabolites with high VIP scores were cortisol , hydroxymethoxyphenylcarboxylic acid - O - sulphate , and isovaleryalanine . - pubmed
The data suggest that meprin beta activity enhances diabetic kidney injury in part by altering the metabolite balance in kidneys , favoring high levels of uremic toxins such as indoxyl sulfate and N - Methyl - pyridone - carboxamide . - pubmed
The results showed that serum protein profiles were linearly significantly increased of serum phosphor , uric acid , aspirate aminotransferase , alanine transaminase and alkaline phosphatase in groups treated with Roundup , as well as the serum concentrations of triglyceride altered after treatment with glyphosate . - pubmed
MALDI - TOF mass spectrometry and NMR spectroscopy revealed the molecular weight of the acetyl - xylogalactan to be 88.5 kDa . - pubmed
Versatile derivatization for GC - MS and LC - MS : alkylation with trialkyloxonium tetrafluoroborates for inorganic anions , chemical warfare agent degradation products , organic acids , and proteomic analysis . - pubmed
Recent analytical applications of trialkyloxonium derivatizations include the high - precision determination of inorganic anions in complex matrices , the qualitative confirmation of chemical warfare agent degradation products in soils , the profiling of carboxylic acids in urine , and the detection of protein post - translational modifications induced by carbon dioxide . - pubmed
Graphical Abstract Alkylation with trialkyloxonium salts for GC - MS and LC - MS analysis . - pubmed
In the case when the fraction of TBCHA is equal or higher than the fraction of VCL , the microgels become almost insensitive to the temperature changes , and the amount of water inside the microgels appeared to be diminishingly small . - pubmed
Phenobarbital , a well - known liver cancer promoter , has been found to promote hepatocyte proliferation via CAR activation . - pubmed
Unigene33312 and Unigene33024 were found to be the most stably expressed genes under salt stress and Cd stress . - pubmed
Moreover , Unigene33024 and PP2A were the most suitable reference genes under Cr stress and Unigene33024 and Sb09g019750 were deemed more suitable reference genes under As stress . - pubmed
The expression patterns of two target genes ( Cu / Zn SOD and CAT ) were used to further verify those selected reference genes under different conditions . - pubmed
In the absence of measured rivaroxaban exposure , exposure estimates were predicted based on individual increases in prothrombin time ( PT ) and the known correlation between rivaroxaban plasma concentrations and PT dynamics . - pubmed
Predicted rivaroxaban exposure and CrCl were significantly associated with both efficacy outcomes in the BID period . - pubmed
AIM : To compare morbidity , postoperative complications , and early survival between liver grafts perfused with IGL-1 and those perfused with histidine - tryptophan - ketoglutarate ( HTK ) solutions . - pubmed
DESIGN : In this cross - sectional study , participants completed abdominal MRI to measure hepatic fat fraction ( HFF ) , oral glucose tolerance test , and PNPLA3 rs738409 genotyping . - pubmed
All the viral proteins were synthesized in the presence of TM , but the glycoproteins were selectively altered in that they migrated faster than normal viral glycoproteins when analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis , suggesting defective glycosylation . - pubmed
Within 1 h after TM addition , [ 14C ] glucosamine incorporation into glycoproteins was inhibited 20 % , whereas [ 35S ] methionine incorporation was unaffected . - pubmed
By 2 to 3 h after TM addition , glucosamine incorporation had fallen to 15 % of control value , with methionine incorporation being 60 % of normal . - pubmed
A simple test system for the separation of staphylococci from micrococci is described , which is based on the ability of staphylococci to produce acid aerobically from glycerol in the presence of 0.4 mug of erythromycin per ml and on their sensitivity to lysostaphin . - pubmed
Very high levels of urinary catecholamines suggested a diagnosis of pheochromocytoma , but no tumour was found at computerized tomography ( CT ) and metaiodobenzylguanidine ( MIBG ) scintigraphy . - pubmed
No tumour was visible at CT or ultrasonography and another MIBG scintigraphy proved negative . - pubmed
These data are consistent with the hypothesis that an increase in the release of catecholamines within the caudal PVN in response to hemorrhage is facilitatory for the release of adrenocorticotropin and/or vasopressin . - pubmed
Multiple short - echo ( 2.5 - ms ) quantitation of in vivo sodium T2 relaxation . - pubmed
Early studies on excised tissue samples revealed that the sodium nucleus exhibited a two - component T2 relaxation . - pubmed
Inhibition of cytotoxic T lymphocyte - mediated lysis and cellular proliferation by isoquinoline sulfonamide protein kinase inhibitors . - pubmed
In addition , suggestive evidence is provided that protein kinase C , or some other enzyme with similar sensitivity to the isoquinoline sulfonamides , plays an important role in cellular proliferation . - pubmed
It is suggested that , as in sarcoidosis , ACE levels are useful in the diagnosis , prognosis and follow - up of Crohn ' s disease , especially in relation to the use of steroid drugs . - pubmed
The effect of mAbs on the alpha BgTx and cholinergic ligand binding properties of the AcChR molecule defined three major categories of mAbs : those that block alpha BgTx and carbamylcholine ( agonist ) binding , but do not block d - tubocurarine ( antagonist ) binding ( 383C , 572C , 370C and 249E ) ; mAb 387D , which blocks agonist binding and partially blocks alpha BgTx and d - tubocurarine binding ; and mAb 247 G , which does not affect agonist binding , blocks at most 50 % of the alpha BgTx binding sites , and decreases the affinity of the high affinity component of d - tubocurarine binding ( Mihovilovic , M. , and Richman , D. P. ( 1984 ) J. Biol . - pubmed
They also indicate the nonequivalence of the two alpha - toxin binding regions of the AcChR molecule and suggest that it is possible to identify epitopes within the alpha BgTx binding region that when bound produce differential effects on the binding of the agonist ( carbamylcholine ) and the antagonist ( d - tubocurarine ) . - pubmed
Comparison of the effects of apamin , a Ca2 + - dependent K + channel blocker , and arylazido aminopropionyl ATP ( ANAPP3 ) , a P2 - purinergic receptor antagonist , in the guinea - pig vas deferens . - pubmed
These studies provide additional support for the view that ANAPP3 antagonizes ATP - induced responses of the guinea - pig vas deferens by blocking P2 - purinergic receptors . - pubmed
By using the immunoadsorbent columns of 6A4 antibody and anti - I - Ab , TaF , in a manner similar to TsF , was demonstrated to be composed of two chains , i.e. - pubmed
Noradrenaline ( 1 microM , in the presence of the uptake inhibitor desipramine ) inhibited K +-induced [ 3H ] NA release by activation of presynaptic alpha - receptors . - pubmed
When the Ca2 + -concentration in the medium was reduced , or the Mg2 + -concentration increased , [ 3H ] NA release appeared to be more susceptible to alpha - receptor mediated inhibition . - pubmed
Veratrine - induced release was also inhibited by alpha - receptor activation . - pubmed
However , [ 3H ] NA release induced by the calcium - ionophore was not affected by alpha - receptor agonists . - pubmed
These results strongly support the view that alpha - receptor activation results in a decrease of the availability of Ca2 + for stimulus - secretion coupling processes . - pubmed
The essential characteristics of pHSG415 and pHSG422 may be summarized as follows : ( 1 ) their genome copy number is low ( 4 - -6 copies / chromosome ) ; ( 2 ) their replication ceases at high temperature and they are rapidly lost from host cells grown at temperatures of 37 degrees C and above ; ( 3 ) the relaxation nick site of pSC101 , which is thought to be synonymous with its origin of transfer replication , is absent from the vectors ; as a consequence , they are not mobilized to a significant extent by co - existing conjugative plasmids that are able to mobilize wild - type pSC101 ; ( 4 ) they contain unique insertion sites for DNA fragments generated by the following restriction endonucleases : EcoRI , XhoI , XmaI , HindIII and PstI ; pHSG415 additionally contains single BamHI , BstEII and HincII sites and may also be used to clone PvuI - generated fragments ; ( 5 ) the plasmids confer upon their host cells resistance to chloramphenicol , kanamycin and ampicillin , and every unique cloning site , except those of BamHI and BstEII , is located within one of these antibiotic - resistance genes . - pubmed
This elevated intracellular calcium concentration is responsible for cytoskeletal modifications which alter cell shape , the activation of phospholipases which results in perpetuation of membrane damage and finally , mitochondrial calcification . - pubmed
These effects of arachidonate are specific to these cations , concentration - dependent , and sensitive to lipoxygenase inhibitors . - pubmed
Arachidonic acid - dependent , but not F - Met - Leu - Phe - dependent , calcium release is sensitive to lipoxygenase inhibitors . - pubmed
Alcohol and Serum creatinine kinase levels . - pubmed
The remaining relaxation elicited by acetylcholine could be blocked with 30 mmol / l KCl or with 10 mumol / l ouabain ( inhibitor of Na + , K (+)- ATPase ) , and , when combined with NG - nitro - L - arginine methyl ester , these interventions abolished acetylcholine - induced relaxation . - pubmed
Tolbutamide at 2 mmol / l or 10 mumol / l glyburide ( blockers of ATP - sensitive potassium channels ) partially inhibited NG - nitro - L - arginine methyl ester - resistant endothelium - dependent relaxation. - pubmed
Theophylline ( 3 x 10(-5)M ) and the phosphodiesterase inhibitor AH 21 - 132 ( 3 x 10(-5)M ) did not alter the enhancement of 3H - overflow caused by either cyclic AMP or adenosine . - pubmed
Forskolin ( 3 x 10(-6)M ) and the phosphodiesterase inhibitors ICI 63 197 ( 10(-4)M ) and AH 21 - 132 ( 3 x 10(-6)-3 x 10(-5)M ) increased stimulation - evoked 3H - overflow . - pubmed
The purified rPhl p V induced specific histamine release of blood basophils from grass pollen - allergic patients . - pubmed
O6 - Benzyl-2 '- deoxyguanosine is a potential antitumor drug modulator that is intended to reduce or eliminate O6 - alkylguanine - DNA alkyltransferase activity in tumors prior to treatment with genotoxic chemotherapeutic alkylating agents . - pubmed
Analysis of venous blood showed the presence of O6 - benzyl-2 '- deoxyguanosine and O6 - benzylguanine at concentrations which are considered to be effective in depleting alkyltransferase activity . - pubmed
The effect of recombinant insulin - like growth factor I on ketone body , lipid and apolipoprotein concentrations and its use to treat ketoacidosis in severe insulin resistance . - pubmed
One month later , the effect of 20 mg rhlGF - I infused daily for 4 days on ketone body concentrations was studied . - pubmed
Further studies of rhlGF-1 use caused a fall in concentrations of cholesterol , triglyceride , VLDL , LDL , and apolipoprotein B. - pubmed
The Sp isomer of 6 - thioethylpurineriboside 3',5 '- phosphorothioate ( 6SEth - cPuMPS ) does not bind to the surface receptor , enters the cell and has relative high affinity for protein kinase A. - pubmed
These results indicate that intracellular cAMP formation and activation of protein kinase A are essential for down - regulation of the surface cAMP receptor . - pubmed
pylori did not differ from those with negative serology with respect to age , duration of acromegaly , duration of octreotide treatment or serum GH level . - pubmed
Cell morphology , protein concentrations , lactate dehydrogenase leakage , energy metabolism , as reflected by specific transport and incorporation of 2 - D -[ 3H ] deoxyglucose , as well as superoxide radical formation were analyzed as a function of time . - pubmed
In this paper we present a new method for the detection of resistance to activated protein C ( APC ) that is based on direct measurement of the effect of APC on the cofactor activity of plasma factor Va. - pubmed
The factor V present in a diluted plasma sample was activated with thrombin and its sensitivity towards APC was subsequently determined by incubation with phospholipids and APC . - pubmed
Addition of 0.2 nM APC and 20 microM phospholipid vesicles to a 1000 - fold diluted sample of thrombin - activated normal plasma resulted in loss of more than 85 % of the cofactor activity factor Va within 6 min . - pubmed
The APC - sr was defined as the ratio of the factor Va cofactor activities determined in thrombin - activated plasma samples after 6 min incubation with or without 0.2 nM APC and was multiplied by 100 to obtain integers ( APC - sr = ? - pubmed
Here , we analyzed the role of free calcium signaling in the induction of CTF-1 transcriptional activity by TGF - beta . - pubmed
TGF - beta induction of CTF-1 is inhibited in cells pretreated with thapsigargin , which depletes the endoplasmic reticulum calcium stores , thus further arguing for the potential relevance of calcium mobilization in TGF - beta action . - pubmed
The immunosuppressants cyclosporin A and FK506 abolish calcineurin - mediated induction of CTF-1 activity . - pubmed
However , TGF - beta still induces the CTF-1 TAD in cells treated with these compounds or in cells overexpressing both calcineurin and DeltaCaMKIV , suggesting that other calcium - sensitive enzymes might mediate TGF - beta action . - pubmed
These results identify CTF / NF - I as a novel calcium signaling pathway - responsive transcription factor and further suggest multiple molecular mechanisms for the induction of CTF / NF - I transcriptional activity by growth factors . - pubmed
Serotonin is strongly related to both types of memory , and particularly , to STM , however , its regulatory role is still unclear . - pubmed
In this study , the effects of tryptophan ( TRY ) restriction on learning and STM were evaluated . - pubmed
This decline in insulin - mediated glucose uptake was accounted for by a decrease in whole body glycolytic rate with no change in the rate of glycogen synthesis . - pubmed
A hydrophobic loop on the surface of SEB and other bacterial superantigens , centered around a conserved leucine ( L45 ) residue , is essential for binding to class II major histocompatibility complex molecules . - pubmed
Vaccines against staphylococcal superantigens should focus on this critical leucine residue . - pubmed
RESULTS : PACAP27 produced dose dependent relaxations of 10 microM rings preconstricted with prostaglandin F2 alpha ( PGF2 alpha ) with half maximal relaxation ( IC50 ) at 17 nM. - pubmed
Pretreatment of the vessels with the ATP sensitive K+ ( KATP ) channel blocker glibenclamide ( 1 microM ) or with the Ca2 + activated K + ( KCa ) channel blocker iberiotoxin ( 100 nM ) inhibited the PACAP27 induced relaxation . - pubmed
In response to decreased oxygen delivery / metabolic " distress " , the RPE may elaborate substances leading to CNV growth . - pubmed
First observation of the EL2 lattice defect in indium gallium arsenide grown by molecular - beam epitaxy . - pubmed
Functionally , the oatp2 - expressing oocytes were found to transport dehydroepiandrosterone sulfate , delta1 opioid receptor agonist [ D - Pen2,D - Pen5 ] enkephalin , Leuenkephalin , and biotin significantly , as well as the substrates previously reported . - pubmed
We have studied the substrate specificity of a recombinant Sgs1 helicase ( amino acid residues 400 - 1268 of the Sgs1 protein ) . - pubmed
DNase I and hydroxyl radical footprinting of the Sgs1 - DNA complex shows that the protein binds specifically to the junction of a double - stranded DNA and its 3 ' overhang . - pubmed
The role played by CAMs and other membrane - associated molecules is discussed in the sense that they are either inductor molecules that turn on different homeobox genes , or downstream products of genes , or both . - pubmed
Kinetics study of a selenium - containing ScFv catalytic antibody that mimics glutathione peroxidase . - pubmed
The steady - state kinetics study and some enzymatic characterization of a selenium - containing scFv catalytic antibody ( Se - scFv2F3 ) were carried out . - pubmed
The obtained kinetics parameters from the kinetics study of Se - scFv2F3 were analyzed and compared with those of native glutathione peroxidase . - pubmed
The parameters measured were : serum levels of albumin ( ALB ) , nitrate , glucose , insulin , corticosterone ( CORT ) , prolactin ( PRL ) and PRL mRNA in the adenopituitaries . - pubmed
Arthritis increased serum NO and reduced ALB levels ; both changes were significantly restored in the FR - AA group . - pubmed
Significant Gal9p - mediated transport could be demonstrated for [ ( 14 ) C ]- uric acid and for [ ( 14 ) C ]- PAH(1 ) . - pubmed
The data of the present study sustain the idea of the involvement of galectin 9 in immune / inflammation processes and in potential - sensitive uric acid translocation . - pubmed
Chromosomal integration of retinoic acid response elements prevents cooperative transcriptional activation by retinoic acid receptor and retinoid X receptor . - pubmed
All - trans - retinoic acid receptors ( RAR ) and 9 - cis - retinoic acid receptors ( RXR ) are nuclear receptors known to cooperatively activate transcription from retinoid - regulated promoters . - pubmed
The effect of 15d - PGJ ( 2 ) on MMP-1 promoter activation was analysed in transient transfection experiments , while electrophoretic mobility shift assays were performed to study the binding activity of the transcription factor AP-1 . - pubmed
PPARgamma activators ( 15d - PGJ ( 2 ) and BRL 49653 ) inhibited IL-1beta - induced MMP-1 synthesis in a dose - dependent manner . - pubmed
Interestingly , 15d - PGJ ( 2 ) reduced both basal and IL-1beta - induced AP-1 binding activity . - pubmed
The reduced pressure response to vasopressin during acute sepsis has directed our interest to the regulation of vasopressin V ( 1A ) receptors . - pubmed
V ( 1A ) receptor expression was downregulated by the cytokines in a nitric oxide - independent manner . - pubmed
Blood pressure dose - response studies after injection of endotoxin showed a diminished responsiveness to the selective V ( 1 ) receptor agonist Phe(2),Ile(3),Orn ( 8 )- vasopressin . - pubmed
We propose that this downregulation of V ( 1A ) receptors contributes to the attenuated responsiveness of blood pressure in response to vasopressin and , therefore , contributes to the circulatory failure in septic shock . - pubmed
In contrast , we found that fasting is associated with increase in the activity of acidic endoglycosidases ( of lysosomal origin ) which degraded hyaluronic acid , chondroitin-4 - sulfate , chondroitin-6 - sulfate and heparin . - pubmed
Increased [ 3H ] thymidine incorporation persisted for three days following treatment with a single dose of GLP-2 . - pubmed
The recombinant strain expressed more than 700 - fold of the beta - gal activity as compared to the wild type strain under induction by maltose . - pubmed
Chloride transport across vesicle and cell membranes by steroid - based receptors . - pubmed
The most significant changes were seen in the MIF + MPA group . - pubmed
In the MPA group , the pre - treatment and post - treatment expression of PR ( 2.9 + /- 1.1 , 1.6 + /- 0.8 ) , ER ( 2.8 + /- 0.9 , 1.4 + /- 0.9 ) , PCNA ( 0.84 + /- 0.10 , 0.60 + /- 0.12 ) , bcl-2 ( 0.236 + /- 0.089 , 0.157 + /- 0.981 ) and CD(44v6 ) ( 4.6 + /- 1.8 , 2.5 + /- 1.9 ) were all decreased ( all P & lt ; 0.01 ) ; the expression of bax ( 0.20 + /- 0.10 , 0.42 + /- 0.07 ) was increased ( P & lt ; 0.01 ) . - pubmed
In the MIF + MPA group , the expression of PR ( 3.2 + /- 1.0 , 0.8 + /- 0.8 ) , ER ( 2.7 + /- 0.9 , 0.7 + /- 0.9 ) , PCNA ( 0.81 + /- 0.09 , 0.25 + /- 0.09 ) , bcl-2 ( 0.225 + /- 0.091 , 0.066 + /- 0.009 ) and CD ( 44v6 ) ( 4.5 + /- 1.9 , 2.7 + /- 1.6 ) were all decreased ( all P & lt ; 0.01 ) ; the expression of bax ( 0.22 + /- 0.06 , 0.59 + /- 0.09 ) was increased ( P & lt ; 0.01 ) ; there were significant different expression of PCNA , ER , PR , bax and bcl-2 as compared with the MIF group and the MPA group , respectively ( all P & lt ; 0.01 ) . - pubmed
The expression of CD(44v6 ) was significantly different ( P & lt ; 0.01 ) between the MIF + MPA group , and the MIF group , but not significantly different between the MIF + MPA group and the MPA group . - pubmed
CONCLUSIONS : The study indicates that high dose progesterone could inhibit the growth , promote apoptosis and inhibit metastasis of endometrial carcinoma , MIF could inhibit the growth and promote apoptosis , MIF + MPA could more strongly inhibit the growth , promote apoptosis and inhibit metastasis of endometrial carcinoma than MIF or MPA , and synergistic effect was observed on the expression of PCNA , ER , PR , bax and bcl-2 . - pubmed
Changes in mRNA expression of gene targets ( estrogen responsiveness , cell cycle progression , apoptosis and aromatase activity ) in tumors induced by the different diets were evaluated . - pubmed
The effect of EPI is prevented by beta ( 2 )- AR blockade and is associated with an increase in G protein - coupled receptor kinase 3 ( GRK3 ) expression . - pubmed
Furthermore , 24 - h EPI or NE treatments that produced desensitization also resulted in a selective 2 - fold up - regulation of GRK3 ; GRK2 was unchanged . - pubmed
The beta - AR antagonist alprenolol ( 1 microM ) and GRK3 antisense ( but not sense ) DNA blocked 0.3 microM EPI- and 10 microM NE - induced desensitization and down - regulation of the alpha ( 2B )- AR as well as GRK3 up - regulation . - pubmed
In conclusion , simultaneous activation of alpha ( 2B )- and beta ( 2 )- ARs results in a 67 - fold decrease in the threshold concentration of EPI required for alpha ( 2B )- AR down - regulation . - pubmed
Backbone residual dipolar coupling ( N - H , Calpha - Halpha , N - C ' , and Calpha - C ' ) data collected in five different media on the B3 IgG binding domain of streptococcal protein G ( GB3 ) have been analyzed by simultaneous refinement of the coordinates and optimization of the magnitudes and orientations of the alignment tensors using single and multiple structure representations . - pubmed
BACKGROUND : S100B , a 20 - kDa , Ca2 + -binding dimer , is a putative intrinsic negative regulator of myocardial hypertrophy expressed after myocardial infarction . - pubmed
AIM : Thiazolidinediones ( TZD ) are a new class of oral antidiabetic drugs that have been shown to inhibit growth of same epithelial cancer cells . - pubmed
Although TZD were found to be ligands for peroxisome proliferator - activated receptor gamma ( PPARgamma ) , the mechanism by which TZD exert their anticancer effect is presently unclear . - pubmed
RESULTS : Exposure to TZD inhibited colony formation in a PPARgamma - dependent manner . - pubmed
CONCLUSION : TZD treatment in pancreatic cancer cells has potent inhibitory effects on growth by a PPAR - dependent induction of pancreatic ductal differentiation . - pubmed
Besides the essential nutrients , non - essential food constituents such as non - digestible carbohydrates may also have an impact on the immune system , especially in the area of the gut - associated lymphoid tissue ( GALT ) . - pubmed
Assessment of an LC - MS method for plasma quantification of the new immunosuppressant FK778 through comparison with HPLC - UV . - pubmed
The aim of this work was to develop an HPLC - MS method to measure FK778 in plasma for pharmacokinetic studies . - pubmed
After precipitation of plasma proteins with acetonitrile , the supernatant was injected onto an analytical RP - C18 column . - pubmed
The aim of this study was to investigate whether aglepristone , a progesterone receptor antagonist , can be used to treat this form of canine acromegaly . - pubmed
Blood samples were collected before MPA administration , immediately before , during , and 3.5 and 5.5 weeks after the last administration of aglepristone for determination of the plasma concentrations of GH and IGF - I. - pubmed
In addition , blood samples for the determination of the 6 - h plasma profile of GH were collected before MPA administration , before aglepristone administration , and 1 week after the last aglepristone treatment . - pubmed
MPA administration resulted in a significant increase of the mean plasma IGF - I concentration , whereas analysis of the pulsatile plasma profile demonstrated a trend ( P=0.06 ) for a higher mean basal plasma GH concentration and a higher mean AUC(0 ) for GH . - pubmed
Treatment with aglepristone resulted in a significant decrease of the mean plasma GH and IGF - I concentrations . - pubmed
Analysis of the pulsatile plasma profile showed a trend ( P=0.06 ) for a lower mean basal plasma GH concentration and a lower mean AUC(0 ) for GH 1 week after the last aglepristone treatment compared with these values before aglepristone administration . - pubmed
Three and a half and 5.5 weeks after the last aglepristone administration the mean plasma IGF - I concentration increased again . - pubmed
administration of the dopamine D1 receptor agonist ( SKF38393 ) , dopamine D2 receptor agonist ( quinpirole ) and dopamine D2 receptor antagonist ( sulpiride ) not only reversed the effect of pre - training morphine treatment , but also increased this action of the drug . - pubmed
administration of dopamine D1 receptor antagonist ( SCH23390 ) prevented the restoration of memory by morphine . - pubmed
In conclusion , the morphine - induced recovery of memory , on the test day , seems to be induced , at least in part , through dopamine receptors . - pubmed
The frequencies of potential susceptibility biomarkers , the UGT1A6 - Arg184Ser and the SULT1A1 - Arg213His polymorphisms , were not associated with urinary BPA levels , either as single genes or in combination . - pubmed
Lower prevalence of the OCT2 Ser270 allele in patients with essential hypertension . - pubmed
The Organic Cation Transporter 2 , OCT2 ( SLC22A2 ) , has been implicated in renal dopamine handling as well as in the inactivation of circulating catecholamines and is supposed to be involved in blood pressure regulation . - pubmed
SLC26A4 ( pendrin , PDS ) is a Na+-independent , Cl-/HCO3-/OH- exchanger that is expressed in the apical regions of type B and non - A , non - B intercalated cells within the cortical collecting duct ( CCD ) , the connecting tubule and the distal convoluted tubule where it mediates HCO3 - secretion and Cl- absorption . - pubmed
SLC26A4 is upregulated with aldosterone analogues and with Cl- restriction . - pubmed
In conclusion , SLC26A4 regulates blood pressure and arterial pH , likely by participating in the renal regulation of net acid and Cl- excretion . - pubmed
PHB overexpression increases the expression of glutathione - S - transferase pi and protects from oxidant - induced depletion of glutathione . - pubmed
Finally , PHB overexpression decreases accumulation of reactive oxygen metabolites , as well as increased permeability induced by oxidative stress in intestinal epithelial cells . - pubmed
To examine the regulation of androgen sensitivity in hair follicles , we focused on androgen receptor co - activator Hic-5 / ARA55 . - pubmed
Western blotting revealed rapid tyrosine phosphorylation of focal adhesion kinase ( FAK ) and Src kinase in response to TGF - beta1 ; inhibition of both of these kinases using pp2 ( 4 - amino-5 -( 4 - chlorophenyl )- 7 -( t - butyl ) pyrazolo [ 3,4 - d ] pyrimidine ) , ameliorates TGF - beta1 - induced monolayer permeability . - pubmed
Activation of FAK / Src requires activation of the epidermal growth factor receptor downstream of the TGF - beta receptors , and is blocked by the epidermal growth factor receptor inhibitor AG1478 . - pubmed
Rearrangement of paxillin and vinculin by TGF - beta1 is not blocked by the FAK / Src inhibitor , pp2 , or by SB431542 inhibition of the TGF - beta type I receptor , anaplastic lymphoma kinase 5 ; however , pp1 ( 4 - Amino-5 -( 4 - methylphenyl )- 7 -( t - butyl ) pyrazolo [ 3,4 - d ] pyrimidine ) , which inhibits both type I and type II TGF - beta receptors , does block paxillin and vinculin rearrangement . - pubmed
Proliferation of SGC cells was determined by methylthiazolyl tetrazolium ( MTT ) assay . - pubmed
EPR spectra of the excited quartet and doublet molecular states of ( tetraphenylporphinato ) zinc ( II ) covalently bounded to 3 -( N - nitronyl - notroxide ) pyridine stable radical are modeled in terms of the spin - Hamiltonian given by the sum of the contributions from the radical and triplet moieties , and the interaction between them . - pubmed
Piracetam and its derivatives improved the outcome by 30.2 % ( 95 % CI = 16.1 - 44.4 ) . - pubmed
Lack of involvement of lipocortin 1 in dexamethasone suppression of IL-1 release . - pubmed
Treatment of peripheral blood monocytes with 2 mug / ml lipopolysaccharide potently increased IL-1beta release ( p = 0.001 ) and dexamethasone ( 10(-7 ) M ) significantly reduced both resting and stimulated IL-1beta release ( p = 0.009 ) . - pubmed
T - cadherin is unique among the family of type I cadherins , because it lacks transmembrane and cytosolic domains , and attaches to the membrane via a glycophosphoinositol anchor . - pubmed
However , Tcad1 is monomeric in the absence and presence of calcium . - pubmed
Specific amino acid replacements compared to classical cadherins reduce the size of the binding pocket for residue 2 and alter the backbone conformation and flexibility around residues 5 and 15 as well as many electrostatic interactions . - pubmed
In addition , we also found that BR regulated the transcription of genes related to many physiological processes , such as photoreaction , ion transportation and some metabolic processes . - pubmed
The 3 - hydroxy group was derivatised to a primary amine and subsequently the thiazolidinone nucleus was generated at the amino functionality through DCC mediated one - pot three - component reaction in good yields . - pubmed
P - glycoprotein ( P - gp ) does not affect melatonin absorption but transported rhodamine 123 , a well - known P - gp substrate . - pubmed
At univariate analysis , both BNP and IL-6 plasma concentrations were predictors of death and heart transplantation , but only BNP was a predictor of death ; however , only creatinine plasma level was an independent predictor of prognosis . - pubmed
Airway mechanosensors [ slowly adapting receptor ( SARs ) and rapidly adapting receptors ] were inhibited by lidocaine , whereas chemosensors ( C - fiber receptors and high - threshold A delta - receptors ) were stimulated . - pubmed
The complete cDNA sequence of Sirt2(T ) is 1059 bp , encoding a deduced protein of 352 amino acids which is 39 amino acids shorter at the N - terminus than Sirt2 . - pubmed
Dietary habits were evaluated using a food frequency questionnaire and the dietary antioxidant capacity was based on published values of Italian foods measured by three different assays : ferric - reducing antioxidant power ( FRAP ) , total radical - trapping antioxidant parameters ( TRAP ) and trolox - equivalent antioxidant capacity ( TEAC ) . - pubmed
RESULTS : Positive associations were observed between dietary antioxidant capacity and adiponectin concentration , as assessed with FRAP ( b+/-s.e.=0.012+/-0.005 , P=0.018 per 1 mmol Fe ( II )/day ) , TRAP ( b+/-s.e.=0.030+/-0.013 , P=0.017 per 1 mmol trolox equivalent / day ) and TEAC ( b+/-s.e.=0.025+/-0.012 , P=0.042 , per 1 mmol trolox equivalent / day ) in multiadjusted analysis . - pubmed
The sensitivity , specificity , accuracy , positive and negative predictive values of FDG PET / CT were respectively 97 % , 92 % , 95 % , 94 % and 96 % . - pubmed
The dialysate - to - plasma ratio for creatinine ( D / P Cr ) was positively correlated to dialysate CTGF concentration and estimated local peritoneal production of CTGF . - pubmed
The nodes of the RD - RIRR network are comprised of models of individual mitochondria that include a mechanism of ROS - dependent oscillation based on the interplay between ROS production , transport , and scavenging ; and incorporating the tricarboxylic acid ( TCA ) cycle , oxidative phosphorylation , and Ca ( 2 + ) handling . - pubmed
Local mitochondrial interaction is mediated by superoxide ( O2.- ) diffusion and the O2.(-)-dependent activation of an inner membrane anion channel ( IMAC ) . - pubmed
Outer membrane proteins ( OMPs ) were analysed by SDS - PAGE and OmpA by matrix - assisted laser desorption ionization time - of - flight / time - of - flight mass spectrometry . - pubmed
CONCLUSIONS : The ' cefpiromase ' or ' cefepimase ' ESBL phenotype of the clinical isolates non - susceptible to cefpirome and cefepime resulted from high expression of TEM-1 or OXA-1 beta - lactamases combined with loss of porins . - pubmed
Based on the comparison of deconvoluted mass spectra with spectra databases as well as experimentally derived retention indices with literature values , 38 metabolites were identified , which belong to the major classes of plant volatiles including C6 - compounds , terpenes ( including modified terpenes ) , aromatic compounds , alcohols and n - alkanes . - pubmed
Our preliminary data indicate that defence related pathways such as the mevalonate and/or alternative isopentenyl pyrophosphate- , the lipoxygenase- ( LOX ) as well as the phenylpropanoid pathway are affected in root galls as a response to phylloxera attack . - pubmed
Single 20- and 40 - mg doses of vorapaxar completely inhibited thrombin receptor activating peptide ( TRAP)-induced platelet aggregation ( & gt;80 % inhibition ) at 1 h and sustained this level of inhibition for & gt;=72 h. Multiple doses yielded complete inhibition on Day 1 ( 5 mg / day ) and Day 7 ( 1 and 3 mg / day ) . - pubmed
Both BMD and histological examinations of femurs showed osteoporotic changes after ovariectomy , while systemic treatment with ZOL restored mineralized bone . - pubmed
Micro - CT examination demonstrated that OVX group showed significant decrease of mineralized bone and implant - bone contact when compared with sham control , whereas both systemic and local treatments of ZOL significantly increased mineralized bone and implant - bone contact in ovariectomized animals . - pubmed
OBJECTIVE : To investigate the selective killing effect of herpes simplex virus - thymidine kinase / ganciclovir ( HSV - tk / GCV ) suicide gene system controlled by human IGF - II P4 promoter on HCC cells in vitro . - pubmed
We show that half of identified E16 - P0 mDA neurons spontaneously generate non - synaptic , intrinsically driven Ca ( 2 + ) spikes and Ca ( 2 + ) plateaus mediated by N- and L - type voltage - gated Ca ( 2 + ) channels . - pubmed
Starting from E18 - P0 , half of the mDA neurons also reliably generate overshooting Na (+ ) spikes with an abrupt maturation at birth ( P0 = E19 ) . - pubmed
At that stage ( E18 - P0 ) , dopaminergic terminals release dopamine in a calcium - dependent manner in the striatum in response to local stimulation . - pubmed
Polymeric copper ( II ) complex , [ Cu ( Hacm )( 2 )( na )( 2 )( H ( 2 ) O )( 2 ) ] [ H ( 2 ) acm ; acetazolamide , na ; nicotinamide ] was synthesized and characterized by spectroscopic ( IR ; infrared spectroscopy , EPR ; electron paramagnetic resonance ) , structural ( XRD ) and voltammetric structural ( CV ) methods . - pubmed
The copper ( II ) compound crystallizes in the triclinic space group P1 , Z=1 , with the unit - cell dimensions : a=7.672 ( 5)A , b=8.681 ( 5)A , c=11.938 ( 5)A , alpha=90.807 ( 7 ) , beta=98.616 ( 5 ) and gamma=110.647 ( 5 ) . - pubmed
The powder EPR spectrum of copper ( II ) complex have indicate that the paramagnetic center is in a tetragonal symmetry with the Cu ( 2 + ) ion having a distorted octahedral geometry . - pubmed
The magnetic properties of the porous metal - organic complex Co ( bpy )( 1.5 )( NO ( 3 ))( 2 ) ( bpy = 4,4 '- bipyridine ) , investigated by SQUID magnetometry , EPR and heat capacity measurements , are reported . - pubmed
Amperometric determination of total phenolic content in wine by laccase immobilized onto silver nanoparticles / zinc oxide nanoparticles modified gold electrode . - pubmed
A method is described for construction of a highly sensitive amperometric biosensor for measurement of total phenolic compounds in wine by immobilizing laccase covalently onto nanocomposite of silver nanoparticles ( AgNPs)/zinc oxide nanoparticles ( ZnONPs ) electrochemically deposited onto gold ( Au ) electrode . - pubmed
Wip1 knockdown or inhibition enhanced DNA damage signaling and resensitized oral SCC cells to cisplatin . - pubmed
Finally , using xenograft tumor models , we showed that Wip1 upregulation promotes tumorigenesis and its inhibition improves the tumor response to cisplatin . - pubmed
Synthesis of novel azo - resveratrol , azo - oxyresveratrol and their derivatives as potent tyrosinase inhibitors . - pubmed
Particularly , introduction of hydroxyl or methoxy group into the 4 - hydroxyphenyl moiety diminished or significantly reduced mushroom tryosinase inhibition . - pubmed
Among the synthesized azo compounds , azo - resveratrol ( 5 ) showed the most potent mushroom tyrosinase inhibition with an IC(50 ) value of IC(50)=36.28 + - 0.72 muM , comparable to that of resveratrol , a well - known tyrosinase inhibitor . - pubmed
Comparisons between SGAs have shown that treatment with olanzapine was associated with greater weight gain and increased cholesterol levels , and that treatment with risperidone was associated with a greater increase in prolactin levels . - pubmed
Peptidomimetics based on the N - terminal stretch of the first 11 amino acids of the PTH have been investigated as potential lead compounds for the treatment of osteoporosis . - pubmed
On the basis of a peptide reported in the literature , referred to here as the Parent Peptide ( H - Aib - Val - Aib - Glu - Ile - Gln - Leu - Nle - His - Gln - Har - NH2 ) , we conducted systematic SAR analyses to investigate the effects of altering peptide hydrophobicity on PTH receptor functional potency as measured by the cAMP ( cyclic adenosine monophosphate ) accumulation and beta - arrestin recruitment assays . - pubmed
Among hydrophobic residues , we found that the Val2 position shows the least flexibility in terms of the SAR studies , whereas the Leu7 position appeared to be most flexible . - pubmed
The somatic cell count ( SCC ) , total bacterial count ( TBC ) in milk , cyclooxygenase ( COX ) activity , phagocytic activity , production of hydrogen peroxide ( H2O2 ) and myeloperoxidase ( MPO ) activity in milk leukocytes were evaluated after intramammary infusion of BRM in quarters inflicted with SCM . - pubmed
Significant enhancement of phagocytic activity , H2O2 and MPO activities indicate the priming of resident milk leukocytes in response to BRM infusion . - pubmed
We explored the association of serum uric acid ( UA ) concentrations with pro - inflammatory state and high - density lipoprotein ( HDL ) dysfunction . - pubmed
Linear regression model revealed fasting triglycerides ( 1.86 mg / dl variance ) , male sex , and gamma - glutamyl transferase and age as covariates of UA levels in women . - pubmed
The major fatty acids were C15 : 0 iso , summed feature 3 ( C16 : 1omega6c and/or C16 : 1omega7c / t ) , C16 : 1omega5c and C15 : 0 anteiso . - pubmed
The effect of age , sex , blood sugar , GCS , CNS , and seizure type on stroke outcome and time to in - hospital death in EPASS was tested on logistic regression and Cox proportional hazard regression . - pubmed
Initial response rates were poor , however they have slowly but steadily improved , such that with the addition of the nucleotide analogue ribavirin and the pegylation of interferon , over 50 % of infected individuals could be cured with a course of therapy . - pubmed
Although the first DAAs were combined with peginterferon and ribavirin , with the rapid progress in the field , it is likely that interferon - free therapy will be available for most patients in the relatively near future . - pubmed
In the short term , peginterferon will be required with either the protease inhibitor simeprevir , or the nucleotide analogue polymerase inhibitor , sofosbuvir , for the treatment of genotype 1 infection . - pubmed
Coincidental metabolic syndrome [ adjusted odds ratio ( aOR ) 2.0 , 95 % confidence interval ( CI ) 1.1 - 3.5 , P = 0.015 ] , central obesity ( aOR 2.0 , 95 % CI 1.0 - 4.1 , P = 0.05 ) and low level of high - density lipoprotein cholesterol ( aOR 1.9 , 95 % CI 1.0 - 3.7 , P = 0.04 ) were associated with liver fibrosis progression independent of change in viral load and ALT level . - pubmed
TLR agonists induced PGE2 in macrophages exclusively via IL-1beta - independent mechanisms . - pubmed
Blocking of TLR4 endocytosis during LPS priming prevented the increase in PGE2 production by exogenous IL-1beta . - pubmed
Our data showed that TLR2 agonists induce PGE2 in monocytes independently from IL-1beta . - pubmed
In contrast , anthocyanin inductive conditions ( AIC ) provided by high sucrose media are characterized by high accumulation of A9 * and A5 relative to other stress conditions . - pubmed
, insulin , glucose , cholesterol ) , insulin signaling components ( e.g. - pubmed
CR decreased plasma levels of insulin , glucose , cholesterol and leptin , and increased hepatic IR beta - subunit and IR pY1158 levels as well as IR , IRS-1 and GLUT-2 gene expression compared to ad libitum feeding , showing a significant beneficial diet intervention effect . - pubmed
On the contrary , GH increased levels of glucose , cholesterol and leptin in plasma , and p - mTOR or p - p38 in livers , and decreased plasma adiponectin and hepatic IR beta - subunit compared to saline treatment . - pubmed
Centrifugal partition chromatography ( CPC ) was used as a fractionation and purification tool , guided by an in vitro adenosine diphosphate ( ADP ) , arachidonic acid or collagen - platelet aggregation assay . - pubmed
Unlike 2G12 , which is strictly specific for the high - density Man9GlcNAc2Asn ( Man9 )-clusters , GNA recognizes a number of N - glycan cryptic sugar moieties . - pubmed
Recently , the 5 - Lipoxygenase ( 5 - LO , a key enzyme metabolizing arachidonic acid to produce leukotrienes ) inhibitors have been showed to protect brain against ischemic damage in animal model of cerebral ischemia . - pubmed
Superoxide dismutase ( SOD , the antioxidant enzyme ) activities and malondialdehyde ( MDA , an oxidative stress biomarker ) contents were detected . - pubmed
NF - kappaBp65 expression and MDA content decreased significantly , and SOD activities increased significantly in hippocampus . - pubmed
Complete work up including physical examination , laboratory and imaging studies ( complete blood count , b - HCG , hormonal levels and ultrasonography ) is needed , and appropriate treatment with combined oral contraceptives is administered accordingly . - pubmed
After surgery , the serum ACTH level was almost normalized , and the dexamethasone ( 1 mg ) suppression test showed significant suppression of ACTH . - pubmed
EXPERT OPINION : Preclinical and retrospective data for erlotinib provide evidence of CNS penetration , and objective responses in the setting of BM from EGFR mutated NSCLC . - pubmed
Phase II / III data however , from non - EGFR mutation enriched populations , have demonstrated no benefit in progression free or overall survival with the addition of erlotinib to metastasis directed radiotherapy . - pubmed
Moreover , we suggest some unexplored pathways such as sphingolipid metabolism , insulin - vesicle trafficking , regulation of transcription / transduction by miRNA-30 , trafficking proteins , and cell cycle proteins that could play important roles in the process mentioned above for further investigation . - pubmed
We further show that iron depletion potentiates the antistaphylococcal activity of HHQ , as well as 2 - heptyl-4 - hydroxyquinoline - N - oxide ( HQNO ) , another AQ that functions as a cytochrome B inhibitor . - pubmed
Linkage disequilibrium between the CYP2C19 * 2 ,* 17 and CYP2C9 * 1 alleles and impact of VKORC1 , CYP2C9 , CYP2C19 gene polymorphisms and gene - gene interactions on warfarin therapy . - pubmed
This study aims to investigate the allele distribution of VKORC1 , CYP2C9 and CYP2C19 , contribution of different allele variants and possible gene - gene interaction on warfarin therapy . - pubmed
Patient with VKORC1 - 1639 G & gt ; A , CYP2C9 * 2 and CYP2C9 * 3 genetic variants need significant lower warfarin dose than patient with wild type allele of VKORC1 1639 G or CYP2C9 * 1 . - pubmed
There is no significant differences between CYP2C19 allele variants for warfarin stable dose and INR & gt ; 5 event . - pubmed
Because of the complete linkage disequilibrium between CYP2C19 * 2 ,* 17 and CYP2C9 * 1 , patient with CYP2C19 * 2 /* 2 , * 2 /* 17 and * 17 /* 17 genotypes tend to have higher warfarin dose than patient with CYP2C19 * 1 /* 1 genotype . - pubmed
Stepwise regression analysis showed that VKORC1 , CYP2C9 , body mass index ( BMI ) , age and gender were included as a factor significantly contributing to warfarin dose , whereas CYP2C19 did not contribute to warfarin dose . - pubmed
No statistically significant interaction between CYP2C9 and VKORC1 on warfarin dose and INR & gt ; 5 event was detected in univariate general linear model analysis . - pubmed
Our study suggests that polymorphic variants of VKORC1 and CYP2C9 affect warfarin dose independently , whereas CYP2C19 did not contribute to warfarin therapy . - pubmed
We found that specific NIR spectral absorbances correlated with MIR measurements of chemical composition , including relative amount of PGA ( R = 0.86 , p = 0.02 ) , collagen ( R = 0.88 , p = 0.03 ) , and proteoglycan ( R = 0.83 , p = 0.01 ) . - pubmed
Then , fasting blood glucose , blood insulin level and insulin resistance index were measured with enzymatic methods . - pubmed
Immunofluorescent labeling was carried out to visualize the insulin receptor substrate 2 ( IRS2 ) , Obese receptor ( Ob - R , a leptin receptor ) , voltage - dependent anion channel protein 1 ( VDAC1 ) , cytochrome C ( cytC ) , 5 - methylcytosine ( 5 - mC ) positive cells in hippocampal CA1 area . - pubmed
The present work provides a detailed study on the structural variants of the tetrasaccharide fragments of this binding site afforded following treatment of a heparin with heparin lyase II . - pubmed
Appropriate concentrations of betaine and L - arginine for subtype - selective inhibition in SNAT - overexpressing cells were identified . - pubmed
This morphological phenotype was rescued by co - expression of TCF4 plus calmodulin in a calcium - dependent manner and by dampening neuronal excitability through co - expression of an inwardly rectifying potassium channel ( Kir2.1 ) . - pubmed
For we believe the first time , we show that N - methyl - d - aspartate ( NMDA ) receptor - dependent Ca2 + transients are instructive to minicolumn organization because Crispr / Cas9 - mediated mutation of NMDA receptors rescued TCF4 - dependent morphological phenotypes . - pubmed
BACKGROUND / AIM : To evaluate the efficacy of conversion surgery following S-1 plus cisplatin ( CS ) or oxaliplatin ( SOX ) chemotherapy . - pubmed
The recombinant protein had 24 kDa apparent molecular mass , suggesting being expressed as a glycoprotein , and was purified from serum - free conditioned medium by the combination of Diethylaminoethanol Sepharose ion exchange and Sephacryl S-200 HR gel filtration . - pubmed
Moreover , hypothyroidism significantly decreased the content of ACh ( 29.8 % ) and activity of AChE ( 27.8 % ) , which were restored to control values by T4 admi - nistration . - pubmed
At the protein level , hypothyroidism increased the levels of syt-1 and SNAP-25 in the PFC , both of which were not restored to control values following T4 administration , while concurrent administration of T4 and DON was able to induce this effect . - pubmed
Dopamine cross - reacts with adrenoreceptors in cortical astrocytes to induce BDNF expression , CREB signaling and morphological transformation . - pubmed
However , it has remained unknown which receptors mediate dopamine - induced BDNF upregulation in astrocytes . - pubmed
We show that in cortical astrocytes BDNF exon IV and exon VI containing mRNAs are induced by dopamine and norepinephrine via CREB - dependent signaling and that this regulation is mediated by a mechanism that is distinct from activity - dependent CREB - mediated activation of BDNF transcription in neurons . - pubmed
We also show that regulation of BDNF promoters IV and VI by catecholamines requires a distal regulatory element in the BDNF locus . - pubmed
Finally , we demonstrate that dopamine - induced astrocyte stellation and induction of CREB signaling are mediated by cross - reaction of dopamine with beta adrenoreceptors . - pubmed
FpvAI is a TonB - dependent transporter ( TBDT ) that actively imports the small siderophore ferripyoverdine ( Fe - Pvd ) by coupling to the proton motive force ( PMF ) via the inner membrane ( IM ) protein TonB1 . - pubmed
The crystal structure of the N - terminal domain of pyoS2 ( pyoS2NTD ) bound to FpvAI ( Kd = 240 pM ) reveals that the pyocin mimics Fe - Pvd , inducing the same conformational changes in the receptor . - pubmed
GGT , AST and ALT levels were tested during weeks 14 - 18 of gestation and oral glucose tolerance test was conducted during 24 - 28 weeks to screen GDM . - pubmed
Here , we discuss the biosynthetic routes toward the N - C macrocyclization of linear peptide precursors , specifically , those transformations that are catalyzed by peptidases . - pubmed
AIM : To evaluate renal safety of TDF according to the Kidney Disease : Improving Global Outcomes ( KDIGO ) guidelines . - pubmed
Participation of fatty acids on the AR through PKA and PKC pathways was evaluated using a specific inhibitor of these enzymes . - pubmed
When spermatozoa were incubated with PKA or PKC inhibitors , there was a significant reduction of arachidonic acid - induced acrosome reaction ( AR ) ( P & lt ; 0.05 ) , and inhibition by PKA inhibitor is stronger than that by PKC inhibitor . - pubmed
Conclusions : Incorporation of unsaturated fatty acids , especially oleic acid , into triglycerides and phospholipids provides prerequisite energy for AR . - pubmed
Cholesterol efflux by arachidonic acid triggers AR . - pubmed
Arachidonic acid activated PKA and PKC pathway participate in induction of the AR . - pubmed
Primary metabolic transformation involved aromatization of the dihydronaphthyridine moiety of metabolite M1 as a major clearance pathway with a second oxidative pathway leading to M4 . - pubmed
Plasma FGF21 concentration consistently increased by threefold only after the two low - protein ( 3 % ) overfeeding diets , one high in carbohydrate ( 75 % ) and the other high in fat ( 46 % ) , with larger increases in FGF21 being associated with greater increases in 24 - h EE . - pubmed
We had previously observed that after severe burn trauma , increased energy expenditure is temporally related to a marked reduction in serum concentrations of both parathyroid hormone and fibroblast growth factor 23 , both of which have phosphaturic effects . - pubmed
Here we used molecular modelling to produce a range of ligand binding domain ( LBD ) point mutants of GluK1 - 3 KAR subunits with and without altered agonist efficacy to further investigate the role of glutamate binding in surface trafficking and activation of homomeric and heteromeric KARs using endoglycosidase digestion , cell surface biotinylation and imaging of changes in intracellular Ca2 + concentration [ Ca2 +]i . - pubmed
However , [ Ca2 +] i imaging indicates that the occupancy of both GluK2 and GluK5 LBDs is required for the full activation of GluK2 / GluK5 heteromeric KAR channels . - pubmed
RESULTS : Compared to model group , OPCRR treatments significantly decreased the serum lipid profiles including total cholesterol , total triglycerides , low - density lipoprotein cholesterol ( LDL - C ) and ox - LDL and increased the high - density lipoprotein cholesterol ( HDL - C ) ; significant increased serum antioxidant enzymes ( SOD and GSH - Px ) and decrease of MDA content as a product of lipid peroxidation ; lowered serum levels of TNF - alpha , IL-1beta , IL-6 , ICAM-1 and VCAM-1 and enhanced IL-10 level ; increased the serum release of nitric oxide and expression of iNOS in aortic , whereas decreased the expression of eNOS . - pubmed
CONCLUSION : OPCRR can improve the progress of atherosclerosis by regulation of lipid metabolism , restoring of the antioxidant capacities , and attenuation of pro - inflammatory cytokines and chemcytokines release , and improving the endothelial dysfunction indicated by nitric oxide system . - pubmed
The mitochondria - associated glycerol-3 - phosphate acyltransferase 1 ( GPAT1 ) catalyzes the first committed step in glycerolipid synthesis , and its activity diverts fatty acids from mitochondrial beta - oxidation . - pubmed
Them2 , a mitochondria - associated acyl - CoA thioesterase , also participates in the trafficking of fatty acids into oxidative versus glycerolipid biosynthetic pathways . - pubmed
Preserved insulin receptor activity was supported by Thr-308 phosphorylation of Akt following GPAT1 overexpression in Them2 -/- hepatocytes . - pubmed
These findings suggest a pathogenic role of Them2 in the biosynthesis of glycerolipid metabolites that promote hepatic insulin resistance . - pubmed
The knockdown of intracellular PRDX6 significantly enhances LOOH and ferroptotic cell death triggered by ferroptosis inducers ( Erastin and RSL-3 ) , which is correlated with the transcriptional activation of heme oxygenase-1 . - pubmed
Moreover , overexpression of heme oxygenase-1 enhances both Erastin- and RSL-3 - triggered LOOH , suggesting that heme oxygenase-1 mediates PRDX6 silencing - enhanced ferroptosis . - pubmed
More importantly , the application of a specific PRDX6 phospholipase A2 ( iPLA2 ) inhibitor , MJ-33 , synergistically enhances the ferroptosis induced by Erastin , suggesting that PRDX6 removes LOOH through its iPLA2 activity . - pubmed
Achieving CR was associated with older age ( p = 0.033 ) , no arthralgia / arthritis at baseline ( p = 0.001 ) , and no prior use of cyclophosphamide ( p = 0.003 ) or hydroxychloroquine ( p = 0.016 ) . - pubmed
Overall , compared with the control group for heart failure , liraglutide treatment significantly can reduce NT - proBNP ( Std . - pubmed
CONCLUSIONS : Liraglutide treatment has an important ability to reduce NT - proBNP and improve 6MWT for heart failure , but shows no important influence on LVEF , LVEDV , LVESV , hospitalization for heart failure , major adverse cardiovascular events , and cardiac death . - pubmed
After SCI , chondroitin sulfate proteoglycans ( CSPGs ) inhibit axon regrowth via putative interactions with the LAR - family protein tyrosine phosphatases , PTPsigma and LAR , localized on the injured axon tips . - pubmed
The saline soil was amended with BC at rates of 0 , 7.5 t ha-1 ( BC1 ) , 15 t ha-1 ( BC2 ) and 30 t ha-1 ( BC3 ) alone or combined with fulvic acid ( 1.5 t ha-1 ) compared with control . - pubmed
Although Na + , Cl- and SO42 - in BC and /or FA treatments significantly decreased , the pH value increased up to 9.0 as the CO32- + HCO3 - content increased . - pubmed
Total organic carbon ( TOC ) and phosphorus ( TP ) responded positively to biochar addition rate . - pubmed
Synthesis , Antimicrobial Activity and Molecular Docking of Novel Thiourea Derivatives Tagged with Thiadiazole , Imidazole and Triazine Moieties as Potential DNA Gyrase and Topoisomerase IV Inhibitors . - pubmed
coli Topoisomerase IV ( IC50 = 0.33 + - 1.25 and 19.72 + - 1.00 microM , respectively ) in comparison with novobiocin ( IC50 values 0.28 + - 1.45 and 10.65 + - 1.02 microM , respectively ) . - pubmed
Moreover , pre - treatment with PLGA / SBN nanoparticles reduced elevated transaminase and bilirubin levels in blood , caspase 3 activation , and morphological histology changes in liver tissue upon DTIC treatment . - pubmed
Gaucher disease , the most prevalent metabolic storage disorder , is caused by mutations in the glucocerebrosidase gene GBA1 , which lead to the accumulation of glucosylceramide ( GlcCer ) in affected cells . - pubmed
With this synthetic mRNA - based rapid differentiation method , we found that the metabolic defect in GD1 patient cells can be rescued by the overexpression of wild - type GBA1 or the treatment with an inhibitor for GlcCer synthesis . - pubmed
We also evaluated for associations between LAKR and the metabolic state of cancer cell lines , given that KRAS has been shown to regulate fatty acid synthesis . - pubmed
FASN inhibitors such as TVB-3664 showed synergistic effects with the KRASG12C inhibitor MRTX849 in LUAD cells with KRASG12C and LAKR , including an in vivo trial using a xenograft model . - pubmed
Type 3 cells are stainable with PAS , ATh , AB , AF , and aniline blue and are distributed in an area comprises of anteroventral RPD and anterodorsal PPD . - pubmed
We measured the binding of [ 14C ] iodoacetamide labeled heavy meromyosin subfragment-1 ( S-1 ) to F - actin by sedimenting the actin - S-1 complex and assaying the radioactivity remaining in the supernatant . - pubmed
Kd was not altered when the troponin - tropomyosin complex was bound on the actin , nor was it affected by free calcium concentration in the range 10(-4 ) to 10(-9 ) M. Measurements of the displacement of labeled S-1 from actin by native S-1 showed labeling had not altered Kd . - pubmed
The fragments Boc - Lys ( Z )- Ile - Cys ( SiPr )- Gly - Lys(Z)(I ) and Boc - Pro - His ( Trt )- Ile - Cys ( Trt )- Arg(Tos ) ( II ) were synthesized conventionally . - pubmed
After the hydrolysis of fragment II with 6N HCl , allo - isoleucine could be detected by gas chromatography and amino - acid analysis . - pubmed
coli BB core was studied by beta - elimination and using the information obtained with KDO - PN . - pubmed
The basal [ Ca2 +]i of cells at rest was not altered by long - term treatment with NGF , neither in the cell bodies nor in the growth cones . - pubmed
The dihydropyridine ( DHP ) nifedipine alone , or together with omega - conotoxin ( omega - CgTX ) , were similarly effective in inhibiting the K (+)-induced increase in [ Ca2+]i in untreated and NGF - treated cell bodies , arguing for a preferential distribution of L - type Ca2 + channels in this cell area . - pubmed
By contrast , after 6 - 7 days exposure to NGF the K (+)-induced initial transient rise of [ Ca2+]i in growth cones was very sensitive to omega - CgTX , whereas nifedipine affected only the sustained rise . - pubmed
During NGF - induced differentiation there is a predominance of N - type Ca2 + channels in growth cones , while Ca2 + stores are of minor importance in these structures . - pubmed
The half - lives of R - and S - flecainide in PMs ( R 19.3 h ; S 16.1 h ) were approximately twice those observed in EMs ( R 8.8 h ; S 9.1 h ) . - pubmed
Specific binding sites for [ 3H]Ro5 - 4864 and [ 3H ] PK 11195 were also assayed and found to cofractionate with each other and in a manner which coincided with the photolabeled PKBS profile . - pubmed
Scatchard analysis of both subpopulations revealed a single class of binding sites for [ 3H ] Ro5 - 4864 with an apparent KD of 14 nM for Q - I and 22 nM for Q - II ; these affinities were slightly lower than those found in mitochondrial membrane preparations used as the starting material for solubilization . - pubmed
The rank order of potency to inhibit [ 3H ] Ro5 - 4864 binding in both subpopulations was PK 11195 greater than Ro5 - 4864 greater than diazepam greater than clonazepam , in connection with the pharmacological specificity of membrane - associated peripheral - type benzodiazepine binding sites . - pubmed
These studies provide direct biochemical evidence that the recognition sites for benzodiazepines and isoquinoline carboxamides cofractionate in unison with the 17 - kD PKBS protein , demonstrating an intimate relationship between this protein and the binding domains for peripheral - type benzodiazepine ligands . - pubmed
Eight of the first nine mutant RTAs studied showed single - amino - acid changes involving residues located in the proposed active - site cleft . - pubmed
The respective rate constants , k1 and k2 , were determined using two superoxide - generating systems ( xanthine oxidase / xanthine as well as ionizing radiation ) . - pubmed
OXANOH oxidation and OXANO reduction are both inhibitable by superoxide dismutase , pH - dependent ( 4.5 - 9.3 ) , and result in a steady state distribution of [ OXANO ] and [ OXANOH ] , independent of their initial concentrations , i.e. - pubmed
the OXANO / OXANOH couple exhibits a metal - independent superoxide dismutase - like function . - pubmed
Native , heparinized , citrated and EDTA blood and platelet - rich plasma ( PRP ) were forced through the filter . - pubmed
ADP- and thrombin - induced aggregation requires GPrIIb / IIIaand fibrinogen . - pubmed
The amino acid sequences of APRTs have been highly conserved throughout evolution . - pubmed
Retention of an elevated CpG dinucleotide content , despite loss of sequence homology , suggests that there may be selection for CpG dinucleotides in these regions and that their maintenance may be important for APRT gene function . - pubmed
Sodium warfarin was routinely used in an amount to reduce the prothrombin activity in a range of 20 - 30 % normal by thrombotest . - pubmed
Heparin therapy was instituted for the prevention of thromboembolism , when prothrombin activity recovered to 50 % normal by thrombotest , in 5 of 7 cases . - pubmed
Heparin therapy was maintained by continuous intravenous infusion to produce ACT of 130 to 150 seconds . - pubmed
Heparin therapy was stopped when prothrombin activity reached a therapeutic level with sodium warfarin . - pubmed
The dopamine hypothesis of schizophrenia received strong support when a two- to three - fold elevation of D2 receptor densities was demonstrated by positron emission tomography ( PET ) and [ 11C ] N - methylspiperone ( [ 11C ] NMSP ) . - pubmed
Preincubation of NK cells with three anti - CD7 mAb ( Leu-9 , 8H8 - 1 , and LAU - A1 ) failed to block the binding of IgM to the Fc mu R. - pubmed
In current - clamp mode , superfusion with L - leucine caused a hyperpolarization while superfusion with L - arginine depolarized the oocyte . - pubmed
In conclusion , rBAT - mediated transport of neutral and dibasic amino acids is associated with net outward or inward currents , respectively , which may be caused by an exchange of neutral with dibasic amino acids . - pubmed
Forty seven units ordered a specific inspired oxygen , the rest ordering " air " as balance . - pubmed
These observations support the suggestion that an unbalanced synthesis of collagenase and TIMP reported to occur following UV - A irradiation or during inflammatory conditions may be mediated by singlet oxygen . - pubmed
By using Pax-5 and Pax-8 recombinant proteins , we demonstrate that the binding activity of the Prd domain is regulated through the oxidation / reduction of conserved cysteine residues . - pubmed
Mass spectrometry analysis and mutagenesis experiments demonstrate that the redox regulation is accomplished through the reversible formation of an intramolecular disulfide bridge involving the cysteines present in the PAI subdomain , whereas the RED subdomain appears quite insensitive to redox potential . - pubmed
Here , we demonstrate that sequences essential for binding to the CAS nuclear export factor are located near the Imp alpha COOH terminus and include a critical acidic motif . - pubmed
Although point mutations introduced into this acidic motif inactivated both CAS binding and Imp alpha nuclear export , a putative leucine - rich nuclear export signal proved to be neither necessary nor sufficient for Imp alpha nuclear export . - pubmed
However , these two NLS - binding sites showed only minimal overlap , thus suggesting that the degeneracy of the Arm repeat region of Imp alpha may serve to facilitate binding to similar but nonidentical basic NLSs . - pubmed
Fourier transform infrared analysis of purified lactose permease : a monodisperse lactose permease preparation is stably folded , alpha - helical , and highly accessible to deuterium exchange . - pubmed
The purification of monodisperse lactose permease has been aided by the development of a lacY gene product containing an amino - terminal six histidine affinity tag . - pubmed
In the novel purification method described here , lactose permease is purified from beta - dodecyl maltoside - solubilized membrane vesicles using three sequential column steps : hydroxyapatite , nickel - nitriloacetic acid ( Ni - NTA ) affinity , and cation - exchange chromatography . - pubmed
Cytochrome P450 - dependent metabolism of arachidonic acid . - pubmed
The cytochrome P450 - dependent metabolism of arachidonic acid , the mechanisms of regulation of stereo- and regiospecificity by cytochrome P450 isoenzymes , and the biological relevance of metabolites of the arachidonic acid cascade is discussed in this review . - pubmed
The comparison between BuXI and BvTI reactive site structure indicates differences at Met59 , Thr66 and Met67 residues . - pubmed
The hydrolysis rate of quenched fluorescence peptide substrates based on BuXI reactive site sequence , Abz - VMIAALPRTMFIQ - EDDnp ( leading peptide ) , by HuPK and porcine pancreatic kallikrein ( PoPK ) is low , but hydrolysis is enhanced with Abz - VMIAALPRTMQ - EDDnp , derived from the leading peptide shortened by removing the dipeptide Phe - Ileu from the C - terminal portion , for HuPK ( Km = 0.68 microM , k(cat)/Km = 1.3 x 10(6 ) M(-1 ) s(-1 ) ) , and the shorter substrate Abz - LPRTMQ - EDDnp is better for PoPK ( Km = 0.66 microM , k(cat)/Km = 2.2 x 10(3 ) M(-1 ) s(-1 ) ) . - pubmed
The contribution of substrate methionine residues to HuPK and PoPK hydrolysis differs from that observed with factor Xa . - pubmed
Fibroblast growth factor-8 ( FGF-8 ) is an important signaling molecule in the generation and patterning of the midbrain , tooth , and limb . - pubmed
First , it converts presumptive retinal pigment epithelium ( RPE ) into NR . - pubmed
However , GABA further elevated CCK ( 10 pM)- , gastrin - releasing peptide ( 100 pM)- , or electrical field stimulation - induced pancreatic secretions of fluid and amylase dose dependently . - pubmed
The GABA ( 30 microM)-enhanced CCK - induced pancreatic secretions were completely blocked by bicuculline ( 10 microM ) , a GABA ( A ) receptor antagonist , but were not affected by saclofen ( 10 microM ) , a GABA ( B ) receptor antagonist . - pubmed
The enhancing effects of GABA ( 30 microM ) on CCK - induced pancreatic secretions were not changed by tetrodotoxin ( 1 microM ) but were partially reduced by cyclo -( 7 - aminoheptanonyl - Phe - D - Trp - Lys - Thr[BZL ] ) ( 10 nM ) , a somatostatin antagonist . - pubmed
Nonsteroidal anti - inflammatory drugs ( NSAIDs ) such as aspirin , indomethacin ( IND ) , ibuprofen and newer cyclooxygenase-2 selective NSAIDs ( e.g. - pubmed
IND pre - treatment significantly inhibited VEGF - induced Egr-1 mRNA expression by 29+/-4 % ( P<0.01 ) and protein expression by 41+/-8 % ( P<0.05 ) . - pubmed
NS-398 inhibited VEGF - induced Egr-1 mRNA and protein expression by 23+/-3 % and 35+/-4 % , respectively ( both P<0.01 ) . - pubmed
Muscular creatine phosphate values were not significantly changed in the TPN group , but the values increased in the TPN + FDP group from 4.06 mumol / g.wt at the beginning to 4.93 mumol / g.wt at 24 h and 5.60 mumol / g.wt at 48 h ( P & lt ; 0.05 ) , with a cytochrome oxidase increase in immunohistochemistry stain . - pubmed
ProMMP-26 has the unique Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp cysteine - switch motif that discriminates this protease from all other matrix metalloproteinases ( MMPs ) known so far . - pubmed
MMPs become functionally active when proteolytic cleavage releases the prodomain and the PRCXXPD sequence and exposes the zinc atom . - pubmed
Here , we report that the Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp motif is not functional in proMMP-26 and consequently is not involved in the activation mechanisms . - pubmed
The autolytic Lys - Lys - Gln(59 ) downward arrow Gln ( 60 )- Phe - His cleavage upstream of the Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp motif induced the proteolytic activity of recombinant proMMP-26 whereas any further cleavage inactivated the enzyme . - pubmed
The AR is a hormone - dependent transcription factor that activates expression of numerous androgen - responsive genes . - pubmed
Earlier labeling studies suggested that nitrogen might be incorporated from a pathway involved in a molybdenum - dependent nitrate reductase . - pubmed
The amino acid sequence deduced from the moeA gene showed significant similarity to members of the MoeA protein family and contains all the structural features that are highly conserved in the putative functional domains of MoeA proteins . - pubmed
mediterranei growth in a minimal medium with nitrate as sole nitrogen source , possibly because nitrate reductase activity is diminished due to disruption of the moeA gene . - pubmed
Compared to the wild type strain , moeA - disrupted mutants lost 95 % of their rifamycin SV production capacity in complex fermentation media . - pubmed
mediterranei , and that the nitrogen assimilation pathway involved in nitrate reductase is the major pathway for the genesis of the amide nitrogen atom in the rifamycin SV molecule . - pubmed
BACKGROUND : Impairment of insulin - stimulated glucose transport is a characteristic of type 2 diabetes . - pubmed
[ Transformation in vivo of dithiopropylthiamine in cocarboxylase ] . - pubmed
BACKGROUND : The differential tolerability profile of various interferon ( IFN )- alpha preparations used in combination with ribavirin for the treatment of chronic hepatitis C needs to be elucidated . - pubmed
OBJECTIVE : To test the hypothesis that fibrinogen / LDL - apheresis is as effective or superior to conventional therapy with plasma expanders and prednisolone in the treatment of SSHL . - pubmed
INTERVENTIONS : Single fibrinogen/ LDL - apheresis infusion of prednisolone ( 250 mg , tapered by 25 mg daily ) , hydroxyethyl starch ( 500 ml , 6 % ) and pentoxifylin ( 400 mg / day ) . - pubmed
CONCLUSIONS : Fibrinogen / LDLapheresis is at least equally effective compared to prednisolone treatment in sudden hearing loss . - pubmed
Direct electrochemistry and electrocatalysis of hemoglobin immobilized on carbon paste electrode by silica sol - gel film . - pubmed
Direct electrochemical and electrocatalytic behaviors of hemoglobin ( Hb ) immobilized on carbon paste electrode ( CPE ) by a silica sol - gel film derived from tetraethylorthosilicate ( TEOS ) were investigated for the first time . - pubmed
The formal potential of Hb heme Fe ( III )/ Fe ( II ) couple varied linearly with the increase of pH in the range of 5.0 - 10.0 with a slope of 49.44 mV pH(-1 ) , which suggests that a proton transfer is accompanied with each electron transfer ( ET ) in the electrochemical reaction . - pubmed
The immobilized Hb displayed the features of peroxidase and gave excellent electrocatalytic performance to the reduction of O2 , NO2 (- ) and H2O2 . - pubmed
The calculated apparent Michaelis - Menten constant was 8.98 x 10(-4)M , which indicated that there was a large catalytic activity of Hb immobilized on CPE by sol - gel film toward H2O2 . - pubmed
On such a ground , the relevance of the starting tip - substrate distance in determining a non - invasive imaging has been investigated for a plastocyanin mutant chemisorbed on Au ( 111 ) electrodes . - pubmed
In the present study , using two - dimensional polyacrylamide gel electrophoresis and quadrupole time - of - flight mass spectrometry , we identified an aspartate ammonia - lyase ( AspA ) which is upregulated upon induction with bronchoalveolar lavage fluid ( BALF ) . - pubmed
In a subset of K (+ ) channels , gating is regulated through the direct binding of ligands by large cytoplasmic RCK domains . - pubmed
Standard detergent solubilization of recombinantly expressed MjK2 required the addition of a high NaCl concentration to the extraction buffer for MjK2 solubilization . - pubmed
Purified MjK2 reconstituted in liposomes generated only limited Rb (+ ) uptake , blocked by BaCl ( 2 ) . - pubmed
As deduced from amino acid sequence , as well as determined by gel - filtration and SDS - polyacrylamide gel electrophoresis , ppBH4 is an active monomer of 46.5 kDa . - pubmed
The difference in fluorescence of the free and enzyme - bound flavin strongly indicates that the increase in enzyme activity in a pH - dependent manner is related to a protein - cofactor interaction . - pubmed
Only one amino acid residue , His75 , has an intrinsic pKa approximately 6.0 and is localized in proximity ( & lt;10 A ) to N5 - N10 of the isoalloxazine ring and , therefore , is able to participate in such an interaction . - pubmed
There is a strong coupling between the fluorescence of the enzyme - bound flavin and the intrinsic tryptophans , as demonstrated by energy transfer between Trp47 and the isoalloxazine ring of flavin adenine dinucleotide ( FAD ) . - pubmed
The pH - induced changes in intrinsic tryptophan and FAD fluorescence indicate that inhibition of the FAD - binding enzyme at low pH is related to dissociation of the flavin cofactor , due to protonation of its adenine moiety . - pubmed
In this work , seven fluorescent , internally quenched , decapeptides have been synthesized using the prototypical cathepsin B selective substrate Z - Phe - Arg - AMC as a lead , and used to identify the structural factors determining the susceptibility of peptides to hydrolysis at acidic and neutral pH values . - pubmed
Peptides containing Ala or Phe at position 6 are good substrates for the enzyme at both pH 5.0 and 7.4 . - pubmed
By contrast , those containing Glu , Asp , Lys or Val are not cleaved at all by cathepsin B at pH 7.4 , and are poorly hydrolyzed at pH 5.0 . - pubmed
C8 is an oligomeric protein composed of a disulfide - linked C8alpha - gamma heterodimer and a noncovalently associated C8beta chain . - pubmed
betaMACPF was also capable of binding independently to erythrocytes carrying C5b-7 . - pubmed
The native enzyme has a molecular weight of 130,000 as measured by gel permeation chromatography , and contains four subunits ( mol wt 31,500 ) as measured by sodium dodecyl sulfate gel electrophoresis . - pubmed
No selenium could be detected in the purified enzyme . - pubmed
The enzyme is active with H ( 2 ) O ( 2 ) and a variety of organic hydroperoxides , including 13 - hydroperoxylinoleic acid , and is specific for GSH as the thiol substrate . - pubmed
The antiapoptotic effect of bortezomib was likely mediated by an increase in hepatic cellular FLICE inhibitory protein ( c - FLIP ) levels , a potent antiapoptotic protein . - pubmed
Consistent with this observation , PCNA immunoreactivity and cyclin A protein expression were also increased with bortezomib treatment . - pubmed
We showed that the cSMAC formed by fusion of microclusters contained more CD45 than microclusters and is a site enriched in lysobisphosphatidic acid , a lipid involved in sorting ubiquitinated membrane proteins for degradation . - pubmed
Calcium signaling via TCR was blocked within 2 min by anti - MHCp treatment and 1 min by latrunculin - A treatment . - pubmed
IDS epimerase does not show significant overall amino acid sequence similarity to any other protein of known three - dimensional structure . - pubmed
The crystal structure of this novel epimerase has been determined by multi - wavelength diffraction to 1.5 A resolution using selenomethionine - substituted enzyme . - pubmed
Role of Scarf and its binding target proteins in epidermal calcium homeostasis . - pubmed
The novel Ca2 + -binding protein , Scarf ( skin calmodulin - related factor ) belongs to the calmodulin - like protein family and is expressed in the differentiated layers of the epidermis . - pubmed
To further understand the functions of Scarf in epidermis in vivo , we altered the epidermal Ca2 + gradient by acute barrier disruption . - pubmed
The expression of Scarf , annexins , calreticulin , and ERp72 were up - regulated by Ca2 + gradient disruption , whereas the expression of 14 - 3 - 3s and nucleolin was reduced . - pubmed
Because annexins , calreticulin , and ERp72 have been implicated in Ca2 + -induced cellular trafficking , including the secretion of lamellar bodies and Ca2 + homeostasis , we propose that the interaction of Scarf with these proteins might be crucial in the process of barrier restoration . - pubmed
Our results suggest that Scarf plays a critical role as a Ca2 + sensor , potentially regulating the function of its binding target proteins in a Ca2 + -dependent manner in the process of restoration of epidermal Ca2 + gradient as well as during epidermal barrier formation . - pubmed
Discovery and structure - activity relationships of new steroidal compounds bearing a carboxy - terminal side chain as androgen receptor pure antagonists . - pubmed
Lead optimization of CH4892280 ( 4 ) , an androgen receptor ( AR ) pure antagonist , was investigated . - pubmed
Twenty micrometer sections were stained by hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils ( anti - mbs-1 ) , activated macrophages / microglia ( anti - ed1 ) , astrocytes ( anti - gfap ) , damaged axons ( anti - betaapp ) and myelin basic protein ( MBP ) . - pubmed
In a new model , with adiponectin as a dependent variable , the HDL cholesterol levels remained in the model ( F = 8.46 ; p & lt ; 0.05 ) , with an increase of 5.38 ( 95 % CI 1.7 - 9.1 ) ng / ml of adiponectin with each 1 mg / dl of HDL cholesterol . - pubmed
Oxamate , an inhibitor of lactate dehydrogenase ( LDH ) , abolished mitochondrial lactate consumption . - pubmed
The presence of estrogen receptors ( ERs ) , as detected by immunohistochemistry ( IHC ) , is a weak prognostic marker of clinical outcome in breast cancer , but a strong predictive marker for response , for example , to tamoxifen - based therapy . - pubmed
Breast cancers with human epidermal receptor protein-2 ( c - erbB-2 ; HER2 ) alterations are critical to identify because such tumors require unique treatment , including the use of targeted therapies such as trastuzumab . - pubmed
faecalis in a synthetic medium with ethanolamine was abolished in the response regulator RR17 mutant strain . - pubmed
Ethanolamine induced a 15 - fold increase in the rate of autophosphorylation in vitro of the HK17 sensor histidine kinase , indicating that this is the ligand recognized by the sensor domain of the kinase . - pubmed
In response to : Benefit of neoral C2 monitoring in de novo cardiac transplant recipients receiving basiliximab induction . - pubmed
We have examined interactions between mitoxantrone ( MXT ) and DNA duplexes or triplexes with different base compositions by using electrospray ionization mass spectrometry ( ESI - MS ) , respectively . - pubmed
Moreover , no sequence selectivity of MXT to different DNA duplexes was found while MXT showed greater affinity to the triplexes that have adjacent TAT or C(+)GC sequences . - pubmed
MEASUREMENT : Maximal isometric voluntary contraction strength of the lower limb and hand with computerized dynamometers , serum 25OHD and parathyroid hormone concentration . - pubmed
F-18 FDG PET / CT findings of a case of complete hydatidiform mole . - pubmed
The interface essentially acts as a proton pump , allowing hydrogen evolution by directly using the aqueous proton . - pubmed
Reentry was easily induced by rapid pacing in cocultures ; treatment with lidocaine , a Na (+ ) channel blocker , significantly decreased reentry rate and CV , increased reentry path length and terminated 30 % of reentrant arrhythmias ( n=18 ) . - pubmed
In contrast , nitrendipine , an L - type Ca ( 2 + ) channel blocker terminated 100 % of reentry episodes while increasing reentry cycle length and path length and decreasing reentry CV ( n=16 ) . - pubmed
Reentry termination by L - type Ca ( 2 + ) channel , but not Na (+ ) channel , blockers suggests a greater Ca ( 2 +)-dependence of propagation . - pubmed
Glutamate - aspartate transporter reduction in Bergmann glia by mutant Atx1 and vulnerability of Purkinje cell to glutamate are both strengthened by Maxer knockdown in Bergmann glia , whereas Maxer overexpression rescues them . - pubmed
International reproducibility of single breathhold T2 * MR for cardiac and liver iron assessment among five thalassemia centers . - pubmed
In this work an LC - DAD - ESI - MS / MS instrument was used to develop a new tandem MS data acquisition strategy for anthocyanin characterization which was subsequently compared to more conventional measurements . - pubmed
It has been shown that the newly developed strategy , multiple reaction monitoring - initiated anthocyanin characterization ( MIAC ) , can successfully be used in anthocyanin analysis and has various advantages compared to some more traditional measurements , such as enhanced selectivity , better signal - to - noise ratio and simplified data evaluation . - pubmed
The plasma concentrations of nitric oxide ( NO ) and tumor necrosis factor - alpha ( TNF - alpha ) were measured using the Griess method and bioassay , respectively . - pubmed
Expression of both proviruses was restricted to androgen - responsive prostate cancer cell lines and LTRs sequences containing steroid hormone - responsive elements bound the androgen receptor and conferred androgen responsiveness to reporter constructs . - pubmed
The NOS-2 was only present in endothelium - denuded aortic rings , as demonstrated by immunoblot with lactone treated rings . - pubmed
Both nitric oxide produced by NOS-2 and K + channels are involved in the relaxant effect of the lactone . - pubmed
Data collected included the underlying disease , basic hematological and biochemical blood test results , APACHE II scores and serum 25 - hydroxyvitamin D [ 25 ( OH ) D ] levels . - pubmed
In addition , we show that acute exposure to NGF , via activation of TrkA , attenuates GABA ( A ) receptor - mediated inhibitory synaptic currents and reduces sensitivity to exogenously applied GABA . - pubmed
These acute actions of NGF display cell type and functional selectivity insofar as ( 1 ) they were found in cholinergic , but not GABAergic , MS / DB neurons , and ( 2 ) glutamate - mediated excitatory synaptic activity as well as AMPA - activated current responses were unaffected . - pubmed
Plasma membrane calcium ATPase 2 ( PMCA2 ) , an important calcium ( Ca ( 2 + ) ) extrusion pump that interacts with synaptic signaling complexes , is most abundantly expressed in PCs compared to other neurons . - pubmed
We focused on Ca ( 2 + ) signaling and the expression of glutamate receptors which play a key role in PC function including synaptic plasticity . - pubmed
We found that the amplitude of depolarization and 2 - amino-3 -( 5 - methyl-3 - oxo-1,2 - oxazol-4 - yl ) propanoic acid receptor ( AMPAR)-mediated Ca ( 2 + ) transients are significantly higher in cultured PMCA2(+/- ) PCs than in PMCA2(+/+ ) PCs . - pubmed
This is due to increased Ca ( 2 + ) influx , since P / Q type voltage - gated Ca(2 + ) channel ( VGCC ) expression was more pronounced in PCs and cerebella of PMCA2(+/- ) mice and VGCC blockade prevented the elevation in amplitude . - pubmed
Vacuolar CAX1 and CAX3 influence auxin transport in guard cells via regulation of apoplastic pH. - pubmed
CATION EXCHANGERs CAX1 and CAX3 are vacuolar ion transporters involved in ion homeostasis in plants . - pubmed
Widely expressed in the plant , they mediate calcium transport from the cytosol to the vacuole lumen using the proton gradient across the tonoplast . - pubmed
Here , we report an unexpected role of CAX1 and CAX3 in regulating apoplastic pH and describe how they contribute to auxin transport using the guard cell 's response as readout of hormone signaling and cross talk . - pubmed
We show that indole-3 - acetic acid ( IAA ) inhibition of abscisic acid ( ABA)-induced stomatal closure is impaired in cax1 , cax3 , and cax1 / cax3 . - pubmed
Both ethylene and 1 - naphthalene acetic acid inhibited ABA - triggered stomatal closure in cax1 , cax3 , and cax1 / cax3 , suggesting that auxin signaling cascades were functional and that a defect in IAA transport caused the phenotype of the cax mutants . - pubmed
We also found that cax1 / cax3 mutants have a higher apoplastic pH than the wild type , further supporting the hypothesis that there is a defect in IAA import in the cax mutants . - pubmed
Accordingly , we were able to fully restore IAA inhibition of ABA - induced stomatal closure in cax1 , cax3 , and cax1 / cax3 when stomatal movement assays were carried out at a lower extracellular pH. - pubmed
Consistent with a role for auxin in vascular specification , the vascular defects in the icu13 mutant were accompanied by reduced expression of auxin transport and auxin perception markers in provascular cells . - pubmed
Achiral pyrazinone - based inhibitors of the hepatitis C virus NS3 protease and drug - resistant variants with elongated substituents directed toward the S2 pocket . - pubmed
Structure - activity relationship data and molecular modeling support occupancy of the S2 pocket from elongated R(6 ) substituents on the 2 ( 1H )- pyrazinone core and several inhibitors with improved inhibitory potency down to Ki = 0.11 muM were identified . - pubmed
RESULTS : The addition of the crystalline amino acids rendered a dose independent three - fold increase of the FPF . - pubmed
The studied enzyme does not deaminate histamine and putrescine and is not sensitive to 10(-2 ) M semicarbaside . - pubmed
Moreover , overexpression of miR-382 significantly attenuated alcohol - induced up - regulation of DRD1 and DeltaFosB , decreased voluntary intake of and preference for alcohol and inhibited the DRD1 - induced action potential responses . - pubmed
A variety of fatty acid esters are now being produced commercially using immobilized lipase in nonaqueous solvents . - pubmed
Here , we identify TRPP2 , a member of the transient receptor potential ( TRP ) superfamily of channel proteins encoded by the pkd2 gene , as a central component of Ca ( 2 + ) signaling in the kidney field . - pubmed
TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular Ca ( 2 + ) entry . - pubmed
We further show that inhibition of Ca ( 2 + ) signaling with an inducible Ca ( 2 + ) chelator also causes downregulation of pax8 , and that pkd2 knockdown results in a severe inhibition of Ca ( 2 + ) transients in kidney field explants . - pubmed
Finally , we show that disruption of RA results both in an inhibition of intracellular Ca ( 2 + ) signaling and of TRPP2 incorporation into the plasma membrane of kidney field cells . - pubmed
We propose that TRPP2 - dependent Ca ( 2 + ) signaling is a key component of pax8 regulation in the kidney field downstream of RA - mediated non - transcriptional control of TRPP2 . - pubmed
A new page on the road book of inorganic mercury in fish body - tissue distribution and elimination following waterborne exposure and post - exposure periods . - pubmed
RESULTS : CNP reduced histological renal tubular damage and apoptosis induced by cisplatin and suppressed plasma blood urea nitrogen and creatinine levels , which were elevated by cisplatin administration . - pubmed
CNP treatment decreased the expression of kidney injury molecule-1 and monocyte chemoattractant protein-1 , which were elevated in the kidney by cisplatin administration . - pubmed
CNP treatment attenuated the decrease in GC - B expression in cisplatin - induced kidney injury . - pubmed
CONCLUSIONS : The present study is the first to show that CNP inhibits nephrotoxicity and kidney cell damage induced by cisplatin . - pubmed
The mechanism of action may involve down - regulation of inflammatory cytokine expression in cisplatin - induced kidney injury and attenuation of apoptosis in renal tubular cells . - pubmed
Osteomodulin ( OMD ) is a member of the small leucine - rich repeat proteoglycan family , which is involved in the organization of the extracellular matrix . - pubmed
However , the potency of bacteriocin was susceptible to digestion with Proteinase K , Pepsin , SDS , EDTA and Urea . - pubmed
Myeloperoxidase , tumor necrosis factor a , interleukin 1beta , PGE2 , LTB4 , cPLA2 , COX-1 , and COX-2 levels were estimated by enzyme - linked immunosorbent assay or Western blot analysis . - pubmed
RESULTS : PMK - S005 significantly attenuated the ethanol - induced gastric damage ; it reduced mucosal inflammatory cytokine production and increased mucus levels . - pubmed
The expression levels of cPLA2 , COX-1 , and COX-2 were decreased by PMK - S005 . - pubmed
A novel hirudin isoform 3 mimetic peptide , named peptide S2 , has been prepared by introduction of a stearic acid modification . - pubmed
Peptide S2 exhibited superior inhibitory activity to hirulog-1 ( Bivariludin ) and showed significantly higher anticoagulant potency in vivo . - pubmed
GMP synthetase ( GMPS ) , a key enzyme in the purine biosynthetic pathway performs catalysis through a coordinated process across two catalytic pockets for which the mechanism remains unclear . - pubmed
Efficacy and mechanism of action of the tyrosine kinase inhibitors gefitinib , lapatinib and neratinib in the treatment of HER2 - positive breast cancer : preclinical and clinical evidence . - pubmed
This review focuses on the effects of three TKIs mostly studied in HER2- positive breast cancer , lapatinib , gefitinib and neratinib . - pubmed
In this work , gamma - mercaptopropyltrimethoxysilane ( gamma - MPTS ) modified Fe3O4@SiO2 magnetic nanoparticles ( MNPs ) was successfully prepared , and characterized by Fourier transform infrared spectrometer ( FT - IR ) , Transmission electron microscope ( TEM ) and Vibrating sample magnetometer ( VSM ) . - pubmed
The sorption performance of the prepared Fe3O4@SiO2@gamma - MPTS MNPs towards methylmercury ( CH3Hg (+ ) ) and inorganic mercury ( Hg ( 2 + ) ) was investigated . - pubmed
It was found that CH3Hg (+ ) and Hg ( 2 + ) could be simultaneously retained on the prepared Fe3O4@SiO2@gamma - MPTS MNPs , and the quantitative elution of CH3Hg (+ ) and total mercury ( THg ) was achieved by using 1.5 mol L(-1 ) HCl containing 0.01 % and 3 % thiourea ( m / v ) , respectively . - pubmed
Based on the above facts , a method of magnetic solid phase extraction ( MSPE ) combined with inductively coupled plasma mass spectrometry ( ICP - MS ) was developed for the speciation of CH3Hg (+ ) and Hg ( 2 + ) . - pubmed
The interrelationship between oxygenated hemoglobin , deoxygenated hemoglobin , total hemoglobin and cerebral oxygen exchange are related to initial dips . - pubmed
In the iron - depleted condition , overexpression of iron homeostasis genes was observed independent of codY deletion ; however , transcription of siderophore and amino acid biosynthesis genes was CodY dependent . - pubmed
Although CodY has a significant regulatory role in central metabolism and the carbon overflow pathway , metabolism - associated genes exhibited CodY - independent expression patterns under glucose starvation . - pubmed
Genes that were differentially expressed in response to CO2 availability showed CodY - dependent regulation , though their maximal expression did require a supply of CO2 / bicarbonate . - pubmed
Baduanjin users also experienced a significant elevation in the serum melatonin levels but showed no significant differences in the Self - Rating Anxiety Scale ( SAS ) score . - pubmed
In this paper , two high atomic number filter materials are studied , namely , gadolinium ( Gd ) and erbium ( Er ) , and compared with Al and Cu filters currently used in breast CT systems . - pubmed
It was observed that spectral shaping using Er and Gd filters substantially increased the dose efficiency ( defined as signal - to - noise ratio of the CHO divided by mean glandular dose ) as compared with kilovolt peak and filter settings used in commercial and prototype breast CT systems . - pubmed
The endothelial nitric oxide synthase cofactor tetrahydrobiopterin shields the remote myocardium from apoptosis after experimental myocardial infarction in vivo . - pubmed
Excess activation of endothelial nitric oxide synthase ( eNOS ) increases its uncoupling potential and results in nitrosative stress via formation of peroxynitrite . - pubmed
Notably , pretreatment with the eNOS cofactor BH4 reduced eNOS activation , prevented excess protein nitrosylation , blunted apoptosis induction , facilitated anti - apoptotic signalling , and eventually prevented apoptosis execution . - pubmed
Evidence is presented that pretreatment with the eNOS cofactor BH4 resulted in less nitrosative stress and weakened apoptotic processes , although the stabilisers contained did participate in this phenomenon . - pubmed
Then , after bleaching labial enamel surfaces of 20 teeth with 15 % carbamide peroxide 6 hours a day for 5 days , they were randomly divided into two groups : A1 and A2 ( n = 10 ) , depending upon whether or not they are treated with antioxidant . - pubmed
Labial enamel surfaces of the remaining 20 teeth were bleached with 38 % hydrogen peroxide before being randomly divided into groups B1 and B2 ( n = 10 ) , again depending on whether or not the antioxidant was used in their treatment . - pubmed
Psilocybin with psychological support improves emotional face recognition in treatment - resistant depression . - pubmed
CONCLUSIONS : Psilocybin with psychological support appears to improve processing of emotional faces in treatment - resistant depression , and this correlates with reduced anhedonia . - pubmed
Design : In a separate study , oral glucose tolerance tests , a 45 - minute bout of exercise , lipid infusions , and euglycemic - hyperinsulinemic clamps ( EHCs ) were performed to investigate the association of myonectin with homeostasis model assessment of insulin resistance ( HOMA - IR ) and T2DM . - pubmed
Plasma myonectin correlated positively with waist / hip ratio , percentage of body fat , triglyceride , fasting blood glucose , 2 - hour blood glucose after glucose overload , fasting insulin , hemoglobin A1c , and HOMA - IR and negatively with the insulin sensitivity index in all of the study population . - pubmed
Circulating myonectin levels were not significantly altered in response to an oral glucose challenge or EHC . - pubmed
In addition , acute elevated free fatty acid levels induced by lipid infusion had no effects on circulating myonectin . - pubmed
GAPDH levels in the preadipocytes were not significantly different at the doses tested for the flavonoids and exogenous GSH . - pubmed
However , significant ( p & lt;.05 ) decreases in phospho - AKT levels in cells treated with quercetin , kaempferol , and GSH at certain doses were observed compared to their respective controls . - pubmed
AUX1 - mediated root hair auxin influx governs SCFTIR1 / AFB - type Ca2 + signaling . - pubmed
This depolarization is strongly impaired in aux1 mutants , indicating that AUX1 is the major transporter for auxin uptake in root hairs . - pubmed
AUX1 - mediated IAA transport , as well as IAA- triggered calcium signals , are blocked by treatment with the SCFTIR1 / AFB - inhibitor auxinole . - pubmed
Our study reveals that the AUX1 transporter , the SCFTIR1 / AFB receptor and the CNGC14 Ca2 + channel , mediate fast auxin signaling in roots . - pubmed
Furthermore , pharmacological inhibition of the endogenous P2Y6 receptor activity by the potently selective P2Y6R antagonist , MRS 2578 , greatly up - regulated pro - inflammatory cytokine interleukin ( IL)-1beta , IL-6 and TNF - alpha gene expression in PBL cells treated with UDP . - pubmed
[ Imaging of parathyroid adenomas with F-18 choline PET - CT ] . - pubmed
Recently , F-18 choline positron emission tomography computed tomography ( choline PET / CT ) has been shown promising results for the diagnostic work up of primary hyperparathyroidism ( pHPT ) suggesting superiority over conventional scintigraphy using Tc99 m sestamibi based protocols using planar dual - phase imaging , SPECT or SPECT / CT . - pubmed
METHODS : This review presents the results of F-18 choline PET / CT on the basis of a literature search using the keywords " primary hyperparathyroidism and choline " , " primary hyperparathyroidism and PET " , " parathyroid adenoma and choline " und " parathyroid adenoma and PET " . - pubmed
RESULTS : 6 studies were identified dealing with the diagnostic impact of choline PET / CT . - pubmed
Localization of single gland adenomas can be achieved with choline PET / CT in 80 up to 96 % of cases . - pubmed
Isocitrate lyase , an essential enzyme for growth on acetate , was expressed under an arabinose inducible promoter . - pubmed
The autonomous downregulation of the expression is based on the gradual oxidation of the arabinose inducer by a glucose dehydrogenase gcd . - pubmed
The aim of this research work was to develop a solid supersaturated self - nanoemulsifying drug delivery system of fenofibrate ( FB ) for enhanced dissolution . - pubmed
METHODS : FB loaded supersaturated self - nanoemulsifying preconcentrate ( superSNEP ) was prepared using dimethyl acetamide ( DMA ) , medium chain triglycerides ( MCT ) , and kolliphor EL . - pubmed
Co - processed excipients ( CPE ) prepared using inorganic microporous silica ( Neusilin US2 , Florite 100 , or Aerosil 200 ) and hydrophilic polymers ( Polyvinyl alcohol , HPMC , and Kollidon VA64 ) were evaluated for flow property , BET surface area , and adsorption capacity . - pubmed
CPE prepared using PVA and Florite 100 ( 1:1 weight ratio ) showed the highest adsorption capacity ( 1 mL / g ) and complete release of oil as depicted by fluorescence study . - pubmed
Pro - inflammatory cytokines concentrations were also decreased by DMF treatment . - pubmed
DMF downregulated the expression of inflammatory ( NF - kB ) and apoptotic ( Bax , Caspase-3 ) genes and protein . - pubmed
DMF treatment increased the gene expression of Nrf2 , HO-1 , NQO1 , Bcl-2 and protein expression of Nrf2 and Bcl2 . - pubmed
To expand the application of double network hydrogel in water treatment , in this study , eco - friendly physically cross - linked double network polymer hydrogel beads ( DAP ) are prepared and studied in depth on the mechanism of Methylene Blue ( MB ) adsorption ; and then the polymer hydrogels are further functionalized by inorganic materials . - pubmed
In general , the ECD of a protein is involved in protein - protein or protein - carbohydrate interactions , whereas the ICD is important for intracellular signaling . - pubmed
We previously reported that GPNMB contributes to the initiation and malignant progression of breast cancer through the hemi - immunoreceptor tyrosine - based activation motif ( hemITAM ) in its ICD . - pubmed
Furthermore , we showed that the tyrosine residue in hemITAM is involved in induction of the stem - like properties of breast cancer cells . - pubmed
MOF exhibited better removal rates than commercial powder activated carbon ( PAC ) , considering pseudo - second order kinetic model . - pubmed
To confirm the practicality of MOF adsorption , we carried out regeneration tests with four adsorption and desorption cycles using acetone as a cleaning solution . - pubmed
Serum metabonomics studies were performed by UPLC - Q - TOF - MS to reveal the dose - dependent biochemical disturbances caused by PMP . - pubmed
In addition , carbachol - induced contraction was reduced in isolated detrusor strips from the L - NAME group , and bladder expression of HIF-1 and connexin 43 showed upregulation . - pubmed
METHODS : We performed a bibliographic research on PUBMED of all studies investigating the use of IV valproate as a treatment of acute mania in BD - I. - pubmed
RESULTS : Overall , the results suggest that IV valproate as a loading therapy is an efficacious , safe and well tolerated treatment for manic episodes , and it is comparable to the oral loading regimen . - pubmed
CONCLUSIONS : IV valproate may represent a valid option for the management of acute mania , with comparable effects in terms of efficacy and safety to the oral valproate . - pubmed
However , larger and more homogeneous studies are warranted in order to collect more precise information on the beneficial effect of IV valproate . - pubmed
The control group did not receive any kind of intervention , one group received functional electrical stimulation of lower limb muscles ( FES group ) , another group practiced Tai Chi exercise ( TCE group ) , and another received functional electrical stimulation of lower limb muscles and practiced Tai Chi exercise ( FES & amp ; TCE group ) . - pubmed
Compared to the control group , only FES group had increased Kansas City Cardiomyopathy Questionnaire ( KCCQ ) score ( P<0.0001 , q=9.06 ) , only the TCE group had decreased heart rate ( P<0.0001 , q=5.72 ) , and decreased peak oxygen consumption was reported in the TCE group ( P<0.0001 , q=9.15 ) and FES & amp ; TCE group ( P<0.0001 , q=10.69 ) . - pubmed
We measure post - shock temperature and pressure in experiments with nonreacting binary mixtures of sulfur hexafluoride and helium ( two dramatically disparate gases ) and show that neither model can accurately predict the observed values , on time scales much longer than that of the shock front passage , due to the models ' implicit assumptions about mixture behavior on the molecular level . - pubmed
However , enthalpy and Tp were higher in the sample without HIU analyzed after OM due to the migration of low melting point triacylglycerols out of the crystalline network . - pubmed
This study introduces for the first time , a new processing technology to reduce OM in a palm kernel fat with high content of saturated fatty acids that is commonly used in confectionery applications . - pubmed
Lactate and BDNF : Key Mediators of Exercise Induced Neuroplasticity ? - pubmed
Current research has shown that ( i ) increased peripheral lactate levels ( following high intensity exercise ) are associated with increased peripheral BDNF levels , ( ii ) lactate infusion at rest can increase peripheral and central BDNF levels and ( iii ) lactate plays a very complex role in the brain 's metabolism . - pubmed
In this review , we summarize the role and relationship of lactate and BDNF in exercise induced neuroplasticity . - pubmed
Conclusions : This review article highlights the role of fundus fluorescein angiography ( FA ) , indocyanine green angiography ( ICG ) , optical coherence tomography ( OCT ) , and optical coherence tomography angiography ( OCTA ) in the diagnosis and management of ocular sarcoidosis . - pubmed
This review article highlights the role of fundus fluorescein angiography ( FA ) , indocyanine green angiography ( ICG ) , optical coherence tomography ( OCT ) , and optical coherence tomography angiography ( OCTA ) in the diagnosis and management of ocular sarcoidosis . - pubmed
PSMA - targeted Radiotracers versus 18F Fluciclovine for the Detection of Prostate Cancer Biochemical Recurrence after Definitive Therapy : A Systematic Review and Meta - Analysis . - pubmed
The comparative performance between fluciclovine and gallium 68 or 18F prostate - specific membrane antigen ( PSMA ) PET / CT , a newer examination , is unclear . - pubmed
Studies of fluciclovine PET / CT or PSMA PET / CT in biochemical recurrence were identified . - pubmed
Pooled detection rates for PSMA and fluciclovine were 45 % ( 95 % CI : 38 % , 52 % ) and 37 % ( 95 % CI : 25 % , 49 % ) , respectively , for a PSA level less than 0.5 ng / mL ( P = .46 ) ; 59 % ( 95 % CI : 52 % , 66 % ) and 48 % ( 95 % CI : 34 % , 61 % ) for a PSA level of 0.5 - 0.9 ng / mL ( P = .19 ) ; and 80 % ( 95 % CI : 75 % , 85 % ) and 62 % ( 95 % CI : 54 % , 70 % ) for a PSA level of 1.0 - 1.9 ng / mL ( P = .01 ) . - pubmed
The Association of ARMC5 with the Renin - Angiotensin - Aldosterone System , Blood Pressure and Glycemia in African Americans . - pubmed
We examined the association of ARMC5 variants with aldosterone , plasma renin activity ( renin ) , blood pressure , glucose and hemoglobin A1c ( A1c ) in community - dwelling AAs . - pubmed
Kernel machine method was used to perform a single joint test to analyze for an overall association between the phenotypes of interest ( aldosterone , renin , systolic and diastolic blood pressure [ SBP , DBP ] , glucose and A1c ) and the ARMC5 single nucleotide variants ( SNVs ) adjusted for age , sex , BMI , and medications ; followed by Baysian Lasso methodology to identify sets of SNVs in terms of associated haplotypes with specific phenotypes . - pubmed
Haplotype TCGCC ( ch16:31476015 - 31476093 ) was negatively associated , whereas haplotype CCCCTTGCG ( ch16:31477195 - 31477460 ) was positively associated with SBP , DBP , and glucose . - pubmed
CONCLUSIONS : ARMC5 variants are associated with aldosterone , PRA , blood pressure , fasting glucose and A1c in community - dwelling AAs , suggesting germline mutations in ARMC5 may underlie cardiometabolic disease in AAs . - pubmed
The linear relationship observed between the thermodynamic force and the thermodynamic flow allowed to establish that MSC - laden in collagen scaffolds were in a near - equilibrium stationary state ( affinity < < RT ) , MSCs were also seeded in solid collagen scaffolds functionalized with the tripeptide Arg - Gly - Asp ( RGD ) . - pubmed
Background and Aims : Mirtazapine is an atypical antidepressant with antagonist activity for serotonin and histamine receptors . - pubmed
Large - scale population screenings are not feasible by applying laborious oral glucose tolerance tests , but using fasting blood glucose ( FPG ) and glycated hemoglobin ( HbA1c ) , a considerable number of diagnoses are missed . - pubmed
Physical , mechanical parameters ( weight loss , decay rate , colour index and firmness ) , phytochemical contents ( ascorbic acid , acidity , soluble solids concentration , titratable acidity , and repining index ) , phenolic enzymes ( peroxidase and polyphenoloxidase ) , pigments ( anthocyanin ) and microbiological analysis ( mesophilic aerobic , yeasts and molds populations ) were detected every other day until the end of the experiment . - pubmed
( B / CH / Nano - TiO2 ) reported a gradual increase in polyphenoloxidase and peroxidase enzyme activities ( 659.45 U / min g ) and ( 20.39 U / min g ) , respectively . - pubmed
Since the ET plasmid - negative substrains still made ET , it was postulated that this residual toxin was made from chromosomal genes . - pubmed
Treatment of cleared lysates from strain UT0002 with ethidium bromide , Pronase , or sodium dodecyl sulfate , but not heat at 60 C , induced conversion of the 56S closed circular ET plasmid to a 38S open circular form as determined after centrifugation on 5 to 20 % neutral sucrose gradients . - pubmed
The binding rate of pyridoxal ' 5 - phosphate ( Pxa - P ) to apotryptophanase and the dissociation rate of the coenzyme from holotryptophanase were able to be determined by following the enzyme activity in continuous flow reactions on a column of immobilized tryptophanase . - pubmed
Immobilized tryptophanase freed of coenzyme restored rapidly its original activity , when the assay mixture containing a given concentration of substrate and Pxa - P was passed through the immobilized enzyme column . - pubmed
This means that Schiff base formation between Pxa - P and tryptophan in the assay mixture has no effect on the binding of Pxa - P to apoenzyme . - pubmed
It was found by an enzymatic study that riboflavin ( FR ) was apparently converted via SF2 to SF1 , and that SF2 was a direct precursor of SF1 . - pubmed
The carboxylic group in SF1 and the aldehyde group in SF2 were detected by spot tests . - pubmed
Elemental analyses and the spectroscopic analyses of the crystalline SF1 supported the existence of carboxylic group in the terminal of its ribityl moiety . - pubmed
From these experimental results , schizoflavins were revealed to be oxidation products of FR that C-5 ' site of its ribityl moiety is oxidized . - pubmed
Consequently , SF1 and SF2 were identified as 7,8 - dimethyl-10 -( 2,3,4 - trihydroxy-4 - carboxybutyl ) isoalloxazine and 7,8 - dimethyl-10 -( 2,3 , 4 - trihydroxy-4 - formylbutyl ) isoalloxazine , respectively . - pubmed
Thus , we postulated that acetylcholine ( ACh ) stimulates bronchial epithelial cells ( BEC ) to release neutrophil and monocyte chemotactic activity ( NCA and MCA ) . - pubmed
BEC released NCA and MCA in response to ACh in a dose - dependent and time - dependent manner . - pubmed
The release of NCA and MCA was inhibited by the lipoxygenase inhibitors , nordihydroguaiaretic acid and diethylcarbamazine . - pubmed
To determine whether smoke might activate BEC to release NCA by stimulating nicotinic ACh receptors , we further characterized the ACh receptors , using nicotine and nicotinic and muscarinic receptor antagonists . - pubmed
Nicotine , the nicotinic receptor antagonist d - tubocurarine , and the M2 receptor antagonist gallamine did not modulate the release of NCA in response to ACh . - pubmed
In contrast , atropine and the M1 receptor antagonist , pirenzepine , inhibited the release of NCA . - pubmed
These data demonstrate that ACh stimulates BEC to release lipoxygenase - derived NCA and MCA through the muscarinic receptor . - pubmed
The deduced amino acid sequence is similar to that of human brain calcineurin beta subunit with respect to containing four putative calcium binding sites . - pubmed
However , distinct differences were found : 1 ) The cloned cDNA had six amino acids polypeptide tail at carboxy - terminal which is absent in human brain calcineurin beta subunit . - pubmed
The ionization states of the 5 '- phosphate group in the various coenzyme forms bound to mitochondrial aspartate aminotransferase . - pubmed
We have carried out a Fourier transform infrared spectroscopic study of mitochondrial aspartate aminotransferase in the spectral region where phosphate monoesters give rise to absorption . - pubmed
The integrated intensity of this band in several enzyme forms ( pyridoxal phosphate , pyridoxamine phosphate , and sodium borohydride - reduced , pyridoxyl phosphate form ) does not change with pH in the range 5 - 9 . - pubmed
The integrated intensity value of this infrared band for the PLP enzyme form before and after reduction with sodium borohydride is close to that given by free PLP at pH 8 - 9 . - pubmed
These results are taken as evidence that in the active site of mitochondrial aspartate aminotransferase the 5 '- phosphate group of PLP remains mostly dianionic even at a pH near 5 . - pubmed
Thus , it is suggested that the chemical shift changes associated with pH titrations of various PLP forms reported in a previous 31P NMR study of this enzyme [ M. E. Mattingly , J. R. Mattingly , and M. Martinez - Carrion ( 1982 ) J. Biol . - pubmed
Since no chemical shift changes were observed in pH titrations of the PMP forms ( lacking an ionizable internal aldimine ) of this isozyme , the Schiff base between PLP and Lys-258 at the active site is the most likely candidate for the ionizing group influencing the phosphorus chemical shift in this enzyme . - pubmed
The MAC - reducing effect of L - DOPA was attenuated by selective antagonism of the D2 dopamine receptor with YM-09151 - 2 while selective blockade of the D1 dopamine receptor with SCH-23390 did not alter L - DOPA 's effect on the MAC for halothane . - pubmed
Inhibitory effects of spermine and spermidine on muscle calpain II . - pubmed
The muscle enzyme calpain II , in contrast to muscle calpain I , was markedly inhibited by millimolar concentrations of the polyamines spermine and spermidine . - pubmed
These results suggest that the polyamines may have possible regulatory effects on the in vivo activity of calpain II enzymes . - pubmed
Even after a 9 - h chase in the presence of brefeldin A , LDL receptor 5 - 10 kDa smaller than the normal mature form was found to be stable . - pubmed
Benazepril significantly reduced systolic blood pressure ( SBP ) and DBP , both supine and standing ( p less than 0.01 ) , while heart rate ( HR ) did not change . - pubmed
These results imply that multidisciplinary treatments in combination of immuno - chemotherapy with IFN - beta , IFN - gamma or vinca alkaloids , hyperthermia and irradiation should be applied for advanced renal cell carcinoma . - pubmed
Dark adaptation increased the calcium - independent CaM kinase activity 2.7 times and calcium - stimulated activity 3.9 times in membrane fractions . - pubmed
In the cytosol , dark adaptation increased calcium- and calmodulin - independent kinase activity 3.3 - fold but did not enhance calcium- and calmodulin - dependent activity . - pubmed
The dose of N - acetylcysteine was 150 mg / kg given in 15 min followed by 50 mg / kg in 4 h and 100 mg / kg over the next 16 h. Liver damage was absent or mild on 13 occasions ( ALT greater than 500 mu / l ) and severe on 5 ( ALT less than 1000 mu / l ) . - pubmed
Application of monoclonal antibody techniques to the study of Na + , K +- ATPase offers a unique opportunity to investigate the molecular basis of sodium potassium transport . - pubmed
However , after higher doses of clonidine ( 0.56 and 1.2 nmol ) a small and short - lasting period of behavioural and ECoG SWS was still evident . - pubmed
Previous studies have demonstrated that two peptides , substance P ( SP ) and substance K ( SK ) , are contained in a common prohormone -- beta - preprotachykinin . - pubmed
This study evaluated the behavioral activity of SK and compared it to the activities of SP , bombesin ( BN ) , and structurally related peptides . - pubmed
The specific rank order of these durations of action were : BN greater than gastrin releasing peptide ( GRP ) = ranatensin ( RT ) greater than neuromedin B ( NMB ) greater than kassinin ( KASS ) = SK = SP and ranged from dose - dependent maxima of approximately 2 min ( SP ) to approximately 100 min ( BN ) . - pubmed
Since the hypothalamus and septum are known to be critical areas for water and electrolyte balance , and blood pressure control , these results suggest that ANP plays a pathophysiological role in these brain regions of SHR . - pubmed
GCR binding is selectively inhibited by methylation of two short , symmetrically arranged clusters of guanine residues within this site . - pubmed
The newly synthesized acid hydrolases acquire Man 6 - P residues by a two - step reaction . - pubmed
Then , N - acetylglucosamine residues are removed by the N - acetylglucosaminyl phosphoglycosidase to generate the Man 6 - P monoester , which is capable of binding the Man 6 - P receptor . - pubmed
Following delivery of enzyme to lysosomes , the enzymes undergo post - lysosomal processing by acid phosphotases , which remove the phosphomonoester groups , and acid proteases which reduce their size and trim off excess polypeptides . - pubmed
Serum type III procollagen peptide in alcoholic liver disease and idiopathic hemochromatosis : its relationship to hepatic fibrosis , activity of the disease and iron overload . - pubmed
Our findings , therefore , weaken the diagnostic value of sPIIIP as an index of connective tissue deposition in the liver , and suggest that , at least in alcohol - related liver disease and IHC , hepatocellular necroinflammation influences the results . - pubmed
SDS - PAGE examination of the microsomes revealed the induction of a cytochrome P-450 isozyme of 53.1 kDa , but the aniline hydroxylase and p - nitroanisole O - demethylase activities of the 9000 g supernatant of liver were not induced . - pubmed
It is important to note that liver hypertrophy , microsomal enzyme induction and the excretion of unchanged cinnamyl anthranilate all have the same dose - threshold for their appearance . - pubmed
Alteration of tyrosine hydroxylase activity in the locus coeruleus after administration of p - chlorophenylalanine . - pubmed
Analysis of HMG proteins by NEPHGE / sodium dodecyl sulfate - gel electrophoresis is thus precluded in samples containing histones . - pubmed
At certain intervals after ovariectomy , a single administration of delta 9 - tetrahydrocannabinol ( THC ; 50 mg / kg ) reduced plasma levels of luteinizing hormone ( LH ) and follicle - stimulating hormone ( FSH ) , while at other times THC had no effect . - pubmed
Administration of THC significantly reduced plasma concentrations of LH and FSH in intact diestrous females . - pubmed
In an additional study , in intact diestrous females injected with testosterone propionate ( TP ; 125 micrograms ) , concommitant exposure to THC did not affect plasma testosterone ( T ) levels , but plasma dihydrotestosterone ( DHT ) levels at 20 and 60 min were significantly higher in animals receiving TP and THC . - pubmed
Effects of THC on plasma androgen levels after exogeneous treatment with TP suggest that THC can alter metabolism or target tissue response to gonadal steroids . - pubmed
Intravenous infusion of 0.7 and 2.9 pmol min-1 kg-1 produced significant elevation of plasma gastrin , cholecystokinin - like immunoreactivity and neurotensin . - pubmed
It was found also to potentiate glucose - dependent insulin secretion . - pubmed
Known liver function tests ( activities of ASAT = L - Aspartate : 2 - oxoglutarate aminotransferase , ALAT = L - Alanine : 2 - oxoglutarate aminotransferase , GLDH = Glutamate - dehydrogenase , LDH = Lactate - dehydrogenase in serum ) are not qualified for detecting minimal histopathological liver alterations induced by aminophenazon . - pubmed
The full - length cDNA as well as genomic clones contain a single open reading frame ( ORF ) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide ( M(r ) ca . - pubmed
Sequence analysis of this clone revealed a continuous unspliced transcript between the region of UL101X and UL102 ; the only in - frame translational stop codon is 2,619 bp downstream from the first ATG in the message . - pubmed
[ Clinical evaluation on the detection of rifampicin specific IgG , IgM ] . - pubmed
The reactions of the positive serum could be inhibited significantly by the addition of RFP , RFP - RSA but not by RSA . - pubmed
Vasoconstriction of about 35 - 40 % of the maximal effect obtained with KCl was found with mononuclear cells activated by thrombin or complement component C5a , but not with unactivated mononuclear cells . - pubmed
HS-142 - 1 , a selective antagonist for ANP biological receptors , inhibited ANP - induced cellular cGMP accumulation and prevented ANP - induced inhibition of both serum - stimulated incorporation of 3H - thymidine and cell proliferation . - pubmed
Des -[ Gln18 , Ser19 , Gly20 , Leu21 , Gly22]-ANP4 - 23 - NH2 ( C - ANP ) failed to inhibit 3H - thymidine incorporation . - pubmed
Oxidative regeneration and selective reduction of native disulfide bonds in the N - terminal half - molecule of ovotransferrin . - pubmed
The denatured , disulfide - reduced form of the N - terminal half - molecule of ovotransferrin was reoxidized with either oxidized dithiothreitol or GSSG and analyzed for the localization of disulfide bonds . - pubmed
Chemical analyses of the reoxidized proteins revealed that the disulfide peptides corresponding to the six native protein disulfides ( SS - I , SS - II , SS - III , SS - IV , SS - V , and SS - VI ) are all regained in the reoxidized protein . - pubmed
The peptide recoveries from the reoxidized proteins were , however , about half of those from the native protein with respect to the two inner disulfides ( SS - IV and SS - V ) in the kringle bridges , but all the disulfide peptides corresponding to the remaining disulfides ( SS - I , SS - II , SS - III , and SS - VI ) were recovered at almost equivalent yields in the native and reoxidized proteins . - pubmed
In addition , on searching for a nonnative disulfide peptide , the two disulfides , Cys171 - Cys174 and Cys174 - Cys182 , which can be accounted for by mispaired bridges of sulfhydryls in SS - IV and SS - V , were detected in the protein reoxidized with oxidized dithiothreitol . - pubmed
Upon disulfide reduction of the native protein with reduced dithiothreitol , both SS - IV and SS - V were selectively cleaved under the same buffer and temperature conditions as in the oxidative refolding . - pubmed
The lower stabilities of the two inner disulfide bonds in the kringle may be related to the lower recoveries of the disulfide peptides from SS - IV and SS - V and the generation of the nonnative disulfide bonds . - pubmed
A 20 - year - old woman from a consanguineous family in the Hunan Province of the People 's Republic of China was diagnosed as having Glanzmann ' s thrombasthenia based on ( 1 ) nearly a lifelong history of epistaxis , gum bleeding , petechiae , and purpura ; ( 2 ) severe menorrhagia resulting in anemia and need for whole - blood transfusion ; ( 3 ) normal coagulation assays ; ( 4 ) prolonged bleeding time ; ( 5 ) absent clot retraction ; ( 6 ) decreased glass bead retention ; ( 7 ) absent platelet aggregation in response to adenine diphosphate , epinephrine , and collagen ; and ( 8) normal initial slope of platelet aggregation in response to ristocetin , but with a diminished maximal extent . - pubmed
Sequence analysis of the patient 's GPIIIa RNA identified a G to A mutation at nucleotide 1219 , predicting a Cys to Tyr substitution at residue 374 . - pubmed
Anti - pseudomonal activity of collagen sponge with liposomal polymyxin B. - pubmed
An antibiotic delivery system has been developed using collagen sponge with liposome - encapsulated polymyxin B. - pubmed
Wounds were dressed with collagen sponge containing liposomal polymyxin B or containing empty liposomes ( with PBS ) as a control . - pubmed
To investigate whether the differences in morphology are due to incorporation of different packaging proteins , spermatozoa nuclear proteins were characterised by acetic acid - urea polyacrylamide gel electrophoresis ( PAGE ) and fractionated by reverse - phase high - pressure liquid chromatography ( HPLC ) . - pubmed
METHODS AND RESULTS : Blood was anticoagulated alone or in combination with citrate , ethylenediaminetetraacetatic acid , corn trypsin inhibitor ( CTI , an inhibitor of activated factor XII ) , heparin , enoxaparin , recombinant tick anticoagulant peptide ( rTAP ) , or recombinant hirudin . - pubmed
Platelet activation in response to adenosine diphosphate ( ADP ) or collagen was detected by assay of P - selectin on the platelet surface delineated by flow cytometry . - pubmed
Although minimal activation was seen without ADP , the fraction of platelets expressing P - selectin in response to ADP was greatest in blood anticoagulated with citrate compared with CTI and all other anticoagulants . - pubmed
ADP - induced platelet activation was greater in blood anticoagulated with heparin compared with an equipotent anti - Xa concentration of enoxaparin . - pubmed
More variable results were seen with collagen , but platelet activation in the presence of citrate was greater than that with CTI . - pubmed
Low molecular weight protein - tyrosine phosphatase tyrosine phosphorylation by c - Src during platelet - derived growth factor - induced mitogenesis correlates with its subcellular targeting . - pubmed
As a consequence of its tyrosine phosphorylation , LMW - PTP significantly increases its catalytic activity . - pubmed
On the other hand we have now demonstrated that the cytoskeleton - associated LMW - PTP acts specifically on a few not yet identified proteins that become tyrosine - phosphorylated in response to the PDGF receptor activation . - pubmed
The cytoplasmic LMW - PTP pool exerts its action within a few minutes from PDGF receptor activation ( short term action ) , while tyrosine phosphorylation of cytoskeleton - associated LMW - PTP lasts for more than 40 min ( long term action ) . - pubmed
To establish the subcellular localization of the PS1 regulation of APP processing to Abeta , fibroblasts from PS1 wild - type ( wt ) or knockout ( KO ) embryos as well as Chinese hamster ovary ( CHO ) cells stably transfected with wt or mutant PS1 were subjected to subcellular fractionation on discontinuous Iodixanol gradients . - pubmed
Bilirubin , formed by activation of heme oxygenase-2 , protects neurons against oxidative stress injury . - pubmed
Heme oxygenase ( HO ) catalyzes the conversion of heme to carbon monoxide , iron , and biliverdin , which is immediately reduced to bilirubin ( BR ) . - pubmed
Two HO active isozymes exist : HO1 , an inducible heat shock protein , and HO2 , which is constitutive and highly concentrated in neurons . - pubmed
We demonstrate a neuroprotective role for BR formed from HO2 . - pubmed
Neurotoxicity elicited by hydrogen peroxide in hippocampal and cortical neuronal cultures is prevented by the phorbol ester , phorbol 12 - myristate 13 - acetate ( PMA ) via stimulation of protein kinase C. - pubmed
One gene out of about 1000 genes analyzed , identified as alpha - tropomyosin , showed an increased transcription level caused by alpha - tocopherol treatment . - pubmed
The aetiology of this development may be related to the patient 's HLA status or possibly to his treatment with the immunosuppressive agent cyclophosphamide ; it is also possible that it is coincidental . - pubmed
All three RNA structures were subsequently subjected to twin refinement in space group P1 , using anisotropic thermal displacement parameters at resolutions of 1.16 , 1.23 and 1.4 A for ALA(wt ) , ALA(I ) and ALA(C70 ) , respectively . - pubmed
Alternatively , the structure of ALA(wt ) was refined in space group C2 assuming twofold disorder of the molecular orientation . - pubmed
Previous studies using pharmacological and behavioral approaches suggested that MDMA treatment leads to desensitization of 5 - HT1B receptors . - pubmed
[ 3H ]- citalopram binding to serotonin transporters in hippocampus was reduced in the high dose protocol , indicating degeneration of forebrain serotonergic fibers . - pubmed
[ 125I ]- Iodocyanopindolol binding to 5 - HT1B sites in hippocampus was also not changed . - pubmed
In Experiment II , high dose MDMA had no effect on 5 - HT1B mRNA in any brain region either 1 or 14 days after treatment . - pubmed
These results suggest that previously described changes in 5 - HT1B function following MDMA treatment involve only posttranscriptional changes in receptor regulation and do not alter 5 - HT1B mRNA levels . - pubmed
Ramipril uniformly decreased deltaGFR and incidence of ESRD in women with either DD ( -39 % and - 100 % ) or II + ID ( -71 % and -82 % ) genotype , and in men ( -25 % and -50 % ) with the DD genotype , but had no beneficial effect in men with the II + ID genotype ( +18 % and +34 % ) . - pubmed
Thus , the relative risk of events ( ESRD ) between conventional and ramipril - treated men was higher in subjects with the DD genotype ( 1.85 ; 0.69 to 4.94 ) and lower in those with the II + /- ID genotype ( 0.71 ; 0.28 to 1.80 ) . - pubmed
Of note , the ACE genotype - related effect of ramipril was still evident even after correction for the above potentially confounding factors ( adjusted RR [ 95 % CI ] : DD , 2.52 [ 0.83 to 7.63 ] , P = 0.10 ; II + ID , 0.35 [ 0.12 to 1.01 ] , P = 0.05 ) . - pubmed
Caveolin-1 is an integral membrane protein of caveolae that is thought to play an important role in both the traffic of cholesterol to caveolae and modulating the activity of multiple signaling molecules at this site . - pubmed
The region between amino acids 71 and 80 controls incorporation of caveolin-1 oligomers into detergent - resistant regions of the Golgi apparatus . - pubmed
The binding ratios for CTAB to collagen , gelatin , DNA - collagen and DNA - gelatin mixtures respectively have also been determined . - pubmed
The conformational structures of different biopolymers are observed to play significant role in macromolecular interactions between protein and DNA in the presence of CTAB . - pubmed
The protein - DNA - CTAB interaction in mixture has been explained in terms of the deviation ( delta ) of ( gamma 2 m ) from ( gamma 2m)id in the presence of a surfactant in bulk . - pubmed
The present retrieval and laboratory investigations have proven , that the morse taper junctions of the MRP - titanium stem are stable and resistant to relevant wear mechanisms . - pubmed
The importance of the highly flexible , N - terminal region of PrP has recently become more widely appreciated , particularly the biological activities associated with its metal ion - binding domain and its potential to form a poly(L - proline ) II ( PPII ) helix . - pubmed
Circular dichroism spectroscopy of an N - terminal peptide , PrP(37 - 53 ) , showed that the PPII helix is formed in aqueous buffer ; as it also contains an Xaa - Pro - Gly consensus sequence , it may act as a substrate for the collagen - modifying enzyme prolyl 4 - hydroxylase . - pubmed
Aberrant CFTR - dependent HCO3- transport in mutations associated with cystic fibrosis . - pubmed
Initially , Cl- conductance in the sweat duct was discovered to be impaired in CF , a finding that has been extended to all CFTR - expressing cells . - pubmed
Subsequent cloning of the gene showed that CFTR functions as a cyclic - AMP - regulated Cl- channel ; and some CF - causing mutations inhibit CFTR Cl- channel activity . - pubmed
The identification of additional CF - causing mutants with normal Cl- channel activity indicates , however , that other CFTR - dependent processes contribute to the disease . - pubmed
Indeed , CFTR regulates other transporters , including Cl(-)-coupled HCO3 - transport . - pubmed
We have examined Cl(-)-coupled HCO3 - transport by CFTR mutants that retain substantial or normal Cl- channel activity . - pubmed
Transcriptional repression by Gal4 - dHDAC1 was blocked by the deacetylase inhibitor , FR901228 , and thus , deacetylase activity correlates with repression . - pubmed
Brain perfusion was measured by ( 99m ) Tc - labeled bicisate ( ECD ) brain SPECT and the images were compared using Statistical Parametric Mapping ( SPM96 ) . - pubmed
BACKGROUND : A recombinant humanized anti - IgE mAb , omalizumab , forms complexes with free IgE , blocking its interaction with mast cells and basophils ; as a consequence , it might be effective in the treatment of asthma . - pubmed
The TTD - determined DHT is a useful predictor of myocardial viability after an anterior AMI . - pubmed
The effect of NCS-1 was specific for GLUT4 and the insulin - responsive aminopeptidase translocation as there was no effect on the trafficking of the cation - independent mannose 6 - phosphate receptor or the GLUT1 glucose transporter isoform . - pubmed
The inhibitory action of NCS-1 was independent of calcium sequestration since neither treatment with ionomycin nor endothelin-1 , both of which elevated the intracellular calcium concentration , restored insulin - stimulated GLUT4 translocation . - pubmed
Endomorphin-1 ( EM-1 ) is a recently isolated endogenous peptide having potent analgesic activity and high affinity and selectivity for the mu - opioid receptor . - pubmed
Trypsin treatment reduced myocyte RF as shown by a decrease in the slope of the RF - SL relationship , and this was accompanied by a downward and leftward shift of the SL - pCa curve , indicative of sensitization of the myofilaments to calcium . - pubmed
In addition , trypsin digestion did not alter the relationship between SL and interfilament spacing ( assessed by cell width ) after calcium activation . - pubmed
The mechanisms of action of these new atypical antipsychotics can be classified into three general groups : a ) binding to D(2 ) together with non - dopaminergic receptors , b ) interaction with dopamine receptor subtypes other than D(2 ) and c ) selective binding to non - dopaminergic systems , such as glutamatergic , sigma , neurotensin , and cannabinoid . - pubmed
All patients underwent complete pulmonary function tests , which included spirometry , body plethysmography , and single - breath carbon monoxide diffusion ( Dlco ) . - pubmed
Adrenaline / noradrenaline ratio in the adrenal glands of the hedgehog during summer activity and hibernation . - pubmed
Comparison of ChIFN - alpha sequences , detected six amino acids substitutions at positions 50 , 58 , 65 , 81 , 181 , and 183 . - pubmed
In addition , both SH - ChIFN - alpha and WJ - ChIFN - alpha were expressed with the N - terminal 6 consecutive histidine residues in a high - level prokaryotic expression system . - pubmed
Localization of ectopic corticotropin - producing carcinoid tumor with use of indium-111 pentetreotide scintigraphy . - pubmed
OBJECTIVE : To report on the diagnosis of ectopic corticotropin ( adrenocorticotropic hormone [ ACTH])-producing bronchial carcinoid tumor by indium-111 pentetreotide ( octreotide scan ) scintigraphy . - pubmed
ACTH values returned to normal after resection of the lesion , and octreotide scans confirmed the completeness of surgical resection . - pubmed
Recent studies reveal that statins stimulate the bone formation , presumably through increasing the production of BMP-2 , an important differentiation factor of osteoblast . - pubmed
Alkaline phosphatase ( ALP ) activity was maintained in the groups cryopreserved with Me ( 2 ) SO and glycerol . - pubmed
In the present study , we have demonstrated SOCE - dependent sarcoplasmic reticulum ( SR ) Ca2 + loading ( load(SR ) ) that was not altered by inhibition of L - type Ca2 + channels , reverse mode Na+/Ca2 + exchange ( NCX ) , or nonselective cation channels . - pubmed
In contrast , lowering the extracellular [ Ca2 + ] to 0 mM or adding either 0.5 mM Zn2 + or the putative store - operated channel ( SOC ) inhibitor SKF-96365 ( 100 microM ) inhibited load(SR ) at rest . - pubmed
Interestingly , inhibition of forward mode NCX with 30 microM KB - R7943 stimulated SOCE significantly and resulted in enhanced load(SR ) . - pubmed
In addition , manipulation of the extracellular and intracellular Na + concentrations further demonstrated the modulatory role of NCX in SOCE - mediated SR Ca2 + loading . - pubmed
Although there is little knowledge of SOCE in cardiomyocytes , the present results suggest that this mechanism , together with NCX , may play an important role in SR Ca2 + homeostasis . - pubmed
These findings may be of particular importance during open heart surgery in neonates , in which uncontrolled SOCE could lead to SR Ca2 + overload and arrhythmogenesis . - pubmed
The macroparameter K(in)app is a composite of several kinetic terms defining the distribution volume of FDOPA in brain ( V(e)D ) and the relative activity of DOPA decarboxylase ( k3D ) . - pubmed
The significant worsening of CPT results did not correlate with changes in FDOPA utilization . - pubmed
CNV was induced using krypton laser photocoagulation of the fundus , and the CNV lesions were subsequently treated with PDT using light doses of 32 , 64 , and 83 s. Enucleated eyes were analyzed with light and transmission electron microscopies , and measurements of CNV size were done on histologic sections and on isolectin B4 - stained choroidal flat mounts . - pubmed
Hsp70 proteins assist refolding of polypeptides in an ATP dependent manner . - pubmed
Ultrasound contrast agent ( SonoVue ) was administered in continuous mode using an infusion pump ( BR - INF 100 , Bracco Research ) . - pubmed
PURPOSE : : Bevacizumab is a monoclonal antibody to vascular endothelial growth factor ( VEGF ) which has been successfully used for the treatment of age - related macular degeneration with choroidal neovascularization . - pubmed
These experiments tested the hypothesis that vitamin E administration attenuates nuclear factor kappaB ( NF - kappaB ) , IL-6 , IL-1beta and tumour necrosis factor alpha ( TNFalpha ) responses in skeletal and cardiac muscle to an inflammatory challenge induced by systemic LPS . - pubmed
Lipopolysaccharide - induced levels of cardiac IL-1beta mRNA and protein and skeletal IL-1beta mRNA were lower with vitamin E than placebo . - pubmed
Lipopolysaccharide - induced NF - kappaB activation and increases in total oxidized proteins were attenuated with vitamin E compared with placebo in both tissues . - pubmed
Vitamin E decreased LPS - induced increases in plasma IL-1beta but not IL-6 compared with placebo . - pubmed
The major results provide the first in vivo evidence that short - term vitamin E administration reduces IL-6 and IL-1beta responses to LPS in skeletal and cardiac muscle and prevents LPS - induced increases in NF - kappaB activation and total oxidized proteins . - pubmed
Although TGA and TAG were compositionally similar , we found a great variation in use of TGA but a very limited range of use of TAG . - pubmed
The frequency of use of TGA in the gene sequences generally increased with the GC content of the chromosome , while the frequency of use of TAG , like that of TAA , was inversely proportional to the GC content of the chromosome . - pubmed
The patterns of use of TAA , TGA and TAG as real stop codons were less biased and less influenced by the GC content of the chromosome . - pubmed
The bias toward TGA but against TAG as a PSC could not be explained either by the preferential usage of specific codons or by the GC contents of individual chromosomes . - pubmed
Peroxisome proliferator - activated receptor - gamma ( PPAR - gamma ) agonists ( thiazolidinediones ) have anti - inflammatory effects and improve endothelium function . - pubmed
Pioglitazone given acutely after transient brain ischemia / reperfusion reduced lesion size and the number of Iba1 - expressing microglia in the ischemic striatum at three days . - pubmed
However , analysis at 6 weeks after MCAo / reperfusion no longer yielded an effect of pioglitazone on either lesion size or Iba1 + cell counts . - pubmed
Regarding functional longer - term outcome , we also did not detect a beneficial effect of pioglitazone on motor function measured either on the pole test or the wire hanging test or on learning and memory in the Morris water maze . - pubmed
Pharmacological preconditioning of mesenchymal stem cells with trimetazidine ( 1 -[ 2,3,4 - trimethoxybenzyl ] piperazine ) protects hypoxic cells against oxidative stress and enhances recovery of myocardial function in infarcted heart through Bcl-2 expression . - pubmed
The preconditioned cells showed a significant protection against H ( 2 ) O ( 2 )-induced loss of cellular viability , membrane damage , and oxygen metabolism accompanied by a significant increase in HIF-1alpha , survivin , phosphorylated Akt ( pAkt ) , and Bcl-2 protein levels and Bcl-2 gene expression . - pubmed
A significant increase in the recovery of myocardial function and up - regulation of pAkt and Bcl-2 levels were observed in hearts transplanted with TMZ - preconditioned cells . - pubmed
Therefore , we tested effects of oligomeric Abeta on membrane fluidity of whole living cells , the impact of exogenous and cellular Abeta on the processing of APP and the role of GM-1 ganglioside . - pubmed
We present evidence that oligoAbeta((1 - 40 ) ) stimulates the amyloidogenic processing of APP by reducing membrane fluidity and complexing with GM-1 ganglioside . - pubmed
Stability and selectivity of a cholesterol - coated C18 stationary phase . - pubmed
Cholesterol loading onto a typical C18 stationary phase is examined . - pubmed
Nitric oxide stimulates myoglobin gene and protein expression in vascular smooth muscle . - pubmed
Mb ( myoglobin ) is a haemoprotein present in cardiac , skeletal and smooth muscle and is primarily responsible for the storage and ' facilitated transfer ' of molecular oxygen from the cell membrane to mitochondria . - pubmed
Overall , exposure of VSMCs ( either in cell culture or intact vessels ) to pathological * NO promotes an up - regulation of the Mb gene and protein , suggesting a feedback relationship between * NO and Mb that regulates the concentration of the potent cell signalling molecule in the vessel wall , similar to the role haemoglobin plays in the vessel lumen . - pubmed
Cocaine self - administration alters the expression of chromatin - remodelling proteins ; modulation by histone deacetylase inhibition . - pubmed
Cocaine self - administration was accompanied by an increased synthesis of Mecp2 , HDAC2 and HDAC11 and by a decreased nuclear localization of HDAC5 and of the phospho - form of HDAC5 , suggesting a nuclear export of this protein in response to the drug . - pubmed
Among the genes we examined , treatment with trichostatin A before each cocaine self - administration session was found to mostly affect Mecp2 and HDAC11 expression . - pubmed
A correlation was found between the modification of Mecp2 and MEF2C gene expression and the reinforcing property of cocaine . - pubmed
We found that insulin and glucose promoted cancer cell proliferation and contributed to chemoresistance . - pubmed
Both drugs affected signalling in the protein kinases B ( AKT)/mammalian target of rapamycin pathway ; metformin activated adenosine monophosphate ( AMP )- activated protein kinase whereas rosiglitazone increased chromosome ten level . - pubmed
Although high insulin and glucose concentrations promoted chemoresistance , the combination of metformin or rosiglitazone with gemcitabine or doxorubicin , resulted in an additional decrease in live cancer cells and increase in apoptosis . - pubmed
A membrane transport protein ( SEC61 ) was also characterised and this demonstrated the utility of the clam sequence data as a resource for examining cold adapted amino acid substitutions . - pubmed
In conclusion , reduced expression of NPY and increased expression of NOS in hippocampus may make significant contributions to CUMS - induced depression . - pubmed
UC - MSCs labeled with carboxyfluorescein diacetate succinimidyl ester ( CFSE ) were found in the lungs and kidneys 1 week post infusion . - pubmed
Silver - silver chloride electrodes placed at Oz , O1 and O2 were referred to Fz . - pubmed
Axotomy - induced changes to the neuronal cytoskeleton and supporting astrocytes during the early stages after transection are delineated by examining the sequence of neurofilament subunit , microtubule ( TUB ) , microtubule associated protein ( MAP ) , glial fibrillary acidic protein ( GFAP ) and terminal deoxynucleotidyl transferase biotin - dUTP nick end labelling ( TUNEL ) modification . - pubmed
Recent discoveries have led to an understanding of the biological role of KIT and platelet - derived growth factor receptor - alpha in GIST and the development of the tyrosine kinase inhibitor imatinib mesylate ( Gleevec , formerly STI-571 ) , one of the most exciting examples of targeted therapy to date . - pubmed
Instability and aggregation problems were circumvented by expression of viral proteins fused to a carrier protein ( thioredoxin A ; TrxA ) , purification via inclusion body formation , urea solubilization , His tag affinity chromatography and direct covalent coupling to microspheres without removal of the elution buffer . - pubmed
The tetragonal prismatic SBU in 2 is connected to eight neighboring SBUs to form a 3 - D MOF of bcu net topology . - pubmed
SynchFastSlow , a metric that is used in clinical depth - of - anesthesia monitors , showed a robust correlation with the loss of consciousness at the induction of propofol GA , but this could be largely explained by power spectral changes without considering cross - frequency coupling . - pubmed
The results suggest that the antidepressant - like activity of DSS is probably mediated by the modulation of central monoamine neurotransmitter systems and the reduction of oxidative stress . - pubmed
Motif analysis predicted direct repeat with a spacer of one nucleotide as the most prevalent RXRalpha binding site . - pubmed
Biological functional analysis of RXRalpha - dependent genes revealed that hepatic RXRalpha deficiency mainly resulted in up - regulation of steroid and cholesterol biosynthesis - related genes and down - regulation of translation- as well as anti - apoptosis - related genes . - pubmed
Mitochondrial swelling has been observed following mitochondrial calcium overload , and studies report that chelation of cytosolic calcium induces extensive mitochondrial division at ER contact sites . - pubmed
Mitochondrial fission following ER stress was abrogated with exposure of cells to the AQP inhibitor mercuric chloride , suggesting the involvement of AQP ( s ) especially AQP8 and AQP9 known to be present in the mitochondrial membrane , in mitochondrial fission . - pubmed
In participants in the highest vs the lowest quintile of nondairy dietary calcium the multivariate relative risk of kidney stones was 0.71 ( 95 % CI 0.56 - 0.92 , p for trend 0.007 ) in HPFS , 0.82 ( 95 % CI 0.69 - 0.98 , p trend 0.08 ) in NHS I and 0.74 ( 95 % CI 0.63 - 0.87 , p trend 0.002 ) in NHS II . - pubmed
When comparing the highest to the lowest quintile of dairy calcium , the multivariate relative risk was 0.77 ( 95 % CI 0.63 - 0.95 , p trend 0.01 ) for HPFS , 0.83 ( 95 % CI 0.69 - 0.99 , p trend 0.05 ) for NHS I and 0.76 ( 95 % CI 0.65 - 0.88 , p trend 0.001 ) for NHS II . - pubmed
We concluded that the oncocytotoxic effect of dopamine was related to the induced polymerization of cytosol actin . - pubmed
MATERIAL AND METHODS : Composite resin ( AeliteFlo , Bisco , Schaumburg , IL , USA ) was inserted into a Class II cavity prepared in the Teflon blocks and was cured with a LED light curing unit ( Dr 's Light , GoodDoctors Co. , Seoul , Korea ) using various light curing modes for 20 s. Polymerization temperature was measured with an infrared thermographic camera ( Thermovision 900 SW / TE , Agema Infra - red Systems AB , Danderyd , Sweden ) for 40 s at measurement spots adjacent to the cavity wall and in the middle of the cavity from the surface to a 4 mm depth . - pubmed
On the other hand , hydralazine , with similar blood pressure lowering to RD , did not significantly suppress the onset of stroke and brain injury in SHRSP . - pubmed
Opiate use ( 7.6 % vs. 33 % ) , imaging ( 6.3 % vs. 20.6 % ) , use of nonsteroidal anti - inflammatory drugs / serotonin agonists at discharge ( 72.7 % vs. 22.2 % ) , and admission rates to hospital ( 22 % vs. 0 % ) were significantly different . - pubmed
The present study was aimed to assess the potential to use the Chk1 and Chk2 inhibitor , AZD7762 , with other anticancer agents in chemotherapy to treat ovarian clear cell carcinoma . - pubmed
We treated the cells with AZD7762 and anticancer agents , then assessed cell viability , cell cycle distribution , apoptosis , and the expression of protein in apoptotic pathways and molecules downstream of the Chk signaling pathways . - pubmed
AZD7762 effectively suppressed the Chk signaling pathways activated by cisplatin , dramatically enhanced expression of phosphorylated H2A.X , cleaved caspase 9 and PARP , decreased the proportion of cells in the gap 0/ gap 1 phase and the synthesis - phase fraction , and increased apoptotic cells . - pubmed
OBJECTIVES : To determine whether a steerable catheter with electrogram guidance ( CS - assist group ) could facilitate access to the coronary sinus ( CS ) during cardiac resynchronization therapy ( CRT ) implantation . - pubmed
The atonal genes are an evolutionarily conserved group of genes encoding regulatory basic helix - loop - helix ( bHLH ) transcription factors . - pubmed
Bilirubin , prothrombin time , creatinine , potassium at admission , and a spontaneous change in bilirubin levels from day 0 to day 7 ( SCBL ) were incorporated into a model for AH to grade the severity in an Asian patient cohort ( MAGIC ) . - pubmed
Cardiac glycoside - mediated effects on NMD are dependent on binding and inhibiting the sodium - potassium ATPase on the plasma membrane and subsequent elevation of intracellular calcium levels . - pubmed
Linear regression modeling was used to evaluate the association of serum leptin level with glomerular filtration rate calculated using the plasma iohexol disappearance curve , demographics , body mass index ( BMI ) , and cardiovascular risk factors , including inflammatory cytokines , insulin resistance , and serum lipid levels . - pubmed
Leptin level also correlated positively with serum triglycerides and insulin resistance ( P & lt ; .001 ) and negatively with serum high - density lipoprotein cholesterol ( P = .002 ) . - pubmed
Leptin level was not associated with glomerular filtration rate calculated using the plasma iohexol disappearance curve or inflammatory cytokines . - pubmed
In multivariate analysis , BMI , age , female sex , and serum triglyceride levels were significantly associated with serum leptin level . - pubmed
Opposite face sensitivity of CeO2 in hydrogenation and oxidation catalysis . - pubmed
OBJECTIVES : To assess if coronary artery calcium ( CAC ) scans influence treatment patterns as reflected by subsequent rates of cardiac imaging and therapeutic interventions , and their effect on ischemic events downstream . - pubmed
Discrete decreased activity in the lower thoracic spine on FDG PET / CT : another respiration - related artifact . - pubmed
The aim of this study was to develop and evaluate the effects of chitosan inserts for sustained release of the angiotensin - converting enzyme 2 ( ACE2 ) activator , diminazene aceturate ( DIZE ) , in experimental glaucoma . - pubmed
Monolayer DIZE loaded inserts ( D+I ) were prepared and characterized through swelling , attenuated total reflectance Fourier transformed infrared spectroscopy ( ATR - FTIR ) , differential scanning calorimetry ( DSC ) and in vitro drug release . - pubmed
Biochemical investigations including GAA , creatine and GAMT enzyme activity measurements are essential to confirm the diagnosis of GAMT deficiency . - pubmed
The present study aims to assess the removal of 3 - amino-5 - methylisoxazole ( AMI ) , a recalcitrant by - product resulting from the biological breakdown of some pharmaceuticals , applying a solar photo - Fenton process assisted by ferrioxalate complexes ( SPFF ) ( Fe3 +/ H2O2 / oxalic acid / UVA - Vis ) and classical solar photo - Fenton process ( SPF ) ( Fe2 +/ H2O2 / UVA - Vis ) . - pubmed
The oxidation power of the classical SPF was assessed at different pH values ( 2.8 - 4.0 ) using 2.0 mg Fe2 + per liter . - pubmed
Furthermore , the effect of AMI concentration ( 2 - 20 mg L-1 ) , presence of inorganic ions ( Cl- , SO42 - , NO3 - , HCO3 - , NH4 + ) , and radical scavengers ( sodium azide and D - mannitol ) on the SPF method at pH 3.5 was also assessed . - pubmed
Oxalic and oxamic acids were identified as the main low - molecular - weight carboxylic acids detected during the pilot - scale SPF reaction . - pubmed
METHODS : Concentration - response curves for phenylephrine ( PE ) were obtained in strips of corpus cavernosum , in absence or after incubation with tiron , tempol or polyethylene glycol ( PEG)-catalase combined or not with tempol . - pubmed
The combination of PEG - catalase and tempol did not alter the contractile response to PE in both groups . - pubmed
HHcy increased O2 (- ) generation and SOD activity , whereas H2O2 concentration was reduced . - pubmed
In this paper , removal of copper ions from aqueous solution using novel xanthan gum ( XG ) hydrogel , xanthan gum - graft-2 - acrylamido-2 - methyl-1 - propane sulfonic acid ( XG - g - P(AMPS ) ) hydrogel and xanthan gum - graft-2 - acrylamido-2 - methyl-1 - propane sulfonic acid / montmorillonite ( XG - g - P ( AMPS )/ MMT ) hydrogel composite were studied . - pubmed
Adsorption process was found to follow Langmuir isotherm model with maximum adsorption capacity of 24.57 , 39.06 and 29.49 mg / g for the XG , XG - g - P(AMPS ) and XG - g - P ( AMPS )/ MMT , respectively . - pubmed
5 '- deoxyadenosine ( dAH ) , S -( 5 '- Adenosyl )- l - cysteine ( AdoCy ) and S -( 5 '- Adenosyl )- l - homocysteine ( AdoHcy ) were competitive inhibitors of MtbBS with the following inactivation parameters : Ki = 24.2 muM , IC50 = 267.4 muM ; Ki = 0.84 muM , IC50 = 9.28 muM ; and Ki = 0.592 muM , IC50 = 6.54 muM for dAH , AdoCy and AdoHcy respectively . - pubmed
Mechanistically , Tets depletion or deficiency increases Dnmt3b and decreases 5hmC levels , resulting in elevated methylation levels at sub - telomeres . - pubmed
During surgery , samples of CSF were collected from the lumbar and ventricular compartments and assayed for chloride , glucose and total protein . - pubmed
Hearts with MR had a 53 % decrease in desmin ( P & lt ; .0001 ) and a 2.6 - fold increase in desmin aggregates ( P & lt ; .0001 ) versus normal , along with substantial , intense perinuclear staining of alpha , beta - unsaturated aldehyde 4 - hydroxynonenal in areas of mitochondrial breakdown and clustering . - pubmed
These findings suggest that mGlu2 is at least in part necessary for the 5 - HT2A receptor - dependent psychosis - like behavioral effects induced by LSD - like drugs . - pubmed
CMG pre - treatment also restored mitochondrial complex I and IV activities inhibited by ROT . - pubmed
CMG induced anti - apoptotic effects was substantiated by decreased phosporylation of JNK3 and c - jun , which in turn decreased the cleavage of pro - caspase 3 . - pubmed
Q - PCR analysis of redox genes showed up - regulation of NOS2 and down - regulation of NQO1 upon ROT exposure and this was attenuated by CMG pre - treatment . - pubmed
Herein , we report a new bottom - up synthetic method utilizing ammonium fluoride ( NH4F ) for the preparation of anhydrous MFx ( CuF2 , FeF3 , and CoF2)/mesoporous carbon ( MSU - F - C ) nanocomposites , whereby a series of metal precursor nanoparticles preconfined in mesoporous carbon were readily converted to anhydrous MFx through simple heat treatment with NH4F under solventless conditions . - pubmed
Here , systematic density functional theory calculations demonstrate that metal sulfide fullerenes Sc2S@Cn have similar cage geometries from C70 to C84 and form a close - knit family of structures related by Endo - Kroto insertion / extrusion of C2 units and Stone - Wales isomerization transformations . - pubmed
SMF of either orientation reduced systolic blood pressure variability in very low frequency domain while downward - oriented SMF also reduced low - frequency and increased high frequency domains . - pubmed
Emergence of RASs relevant only to the polymerase non - nucleoside inhibitors ( NNI ) was detected , and these lay within CD8 + T - cell epitopes . - pubmed
It provides an alternative MRS tool for in vivo study of metabolic coupling relationship between aerobic and anaerobic glucose metabolisms in brain under physiopathological states . - pubmed
Both Pten and Nras are downstream mediators of receptor tyrosine kinase activation that plays important roles in controlling cell survival and proliferation . - pubmed
At the molecular level , lipid droplet formation was primarily contributed by upregulated expression of genes responsible for lipogenesis and fatty acid sequestration , such as Srebpf1 , Acc , Pparg and its downstream targets . - pubmed
From the in silico prediction of PRAME gene structure , we identified the putative myeloid zinc finger 1 ( MZF1 ) binding sites , which overlap with a CpG - rich region located in the first intron . - pubmed
Upon treatment with a DNA methylation inhibitor , 5 - aza-2 '- deoxycitidine ( 5 - azaC ) , together with ectopic expression of MZF1 , PRAME expression was significantly enhanced at both the protein and mRNA levels . - pubmed
More pronounced MZF1 binding to the PRAME DNA was observed in the presence of 5 - azaC. - pubmed
Molecular mechanism of PD-1 / PD - L1 blockade via anti - PD - L1 antibodies atezolizumab and durvalumab . - pubmed
In 2016 and 2017 , monoclonal antibodies targeting PD - L1 , including atezolizumab , durvalumab , and avelumab , were approved by the FDA for the treatment of multiple advanced cancers . - pubmed
Recently , the crystal structures of PD - L1 in complex with BMS-936559 and avelumab have been determined , revealing details of the antigen - antibody interactions . - pubmed
However , it is still unknown how atezolizumab and durvalumab specifically recognize PD - L1 , although this is important for investigating novel binding sites on PD - L1 targeted by other therapeutic antibodies for the design and improvement of anti - PD - L1 agents . - pubmed
Here , we report the crystal structures of PD - L1 in complex with atezolizumab and durvalumab to elucidate the precise epitopes involved and the structural basis for PD-1 / PD - L1 blockade by these antibodies . - pubmed
Thioredoxin-1 maintains mechanistic target of rapamycin ( mTOR ) function during oxidative stress in cardiomyocytes . - pubmed
Thioredoxin 1 ( Trx1 ) is a 12 - kDa oxidoreductase that catalyzes thiol - disulfide exchange reactions to reduce proteins with disulfide bonds . - pubmed
Mechanistic target of rapamycin ( mTOR ) is a serine / threonine kinase that regulates cell growth , metabolism , and survival . - pubmed
In response to H2O2 treatment in cardiomyocytes , mTOR exhibited a high molecular weight shift in non - reducing SDS - PAGE in a 2 - mercaptoethanol - sensitive manner , suggesting that mTOR is oxidized and forms disulfide bonds with itself or other proteins . - pubmed
Immune complex kinase assays disclosed that H2O2 treatment diminished mTOR kinase activity , indicating that mTOR is inhibited by oxidation . - pubmed
Of note , Trx1 overexpression attenuated both H2O2 - mediated mTOR oxidation and inhibition , whereas Trx1 knockdown increased mTOR oxidation and inhibition . - pubmed
Moreover , Trx1 normalized H2O2 - induced down - regulation of metabolic genes and stimulation of cell death , and an mTOR inhibitor abolished Trx1 - mediated rescue of gene expression . - pubmed
H2O2 - induced oxidation and inhibition of mTOR were attenuated when Cys-1483 of mTOR was mutated to phenylalanine . - pubmed
These results suggest that Trx1 protects cardiomyocytes against stress by reducing mTOR at Cys-1483 , thereby preserving the activity of mTOR and inhibiting cell death . - pubmed
The temporal effect of transforming growth factor beta 3 ( TGFbeta3 ) and fibroblast growth factor 2 ( FGF2 ) on the derivation of proliferative chondrocytes from MSCs in three - dimensional agarose was investigated by manipulating the duration of TGFbeta3 and FGF2 treatment . - pubmed
Interleukin-33 - Activated Islet - Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production . - pubmed
Here we show that interleukin ( IL )- 33 was produced by islet mesenchymal cells and enhanced by a diabetes milieu ( glucose , IL-1beta , and palmitate ) . - pubmed
IL-33 promoted beta cell function through islet - resident group 2 innate lymphoid cells ( ILC2s ) that elicited retinoic acid ( RA)-producing capacities in macrophages and dendritic cells via the secretion of IL-13 and colony - stimulating factor 2 . - pubmed
Disruption of TFGbeta - SMAD3 pathway by the nuclear receptor SHP mediates the antifibrotic activities of BAR704 , a novel highly selective FXR ligand . - pubmed
The farnesoid - x - receptor ( FXR ) is a bile acid sensor that counteracts HSCs transdifferentiation . - pubmed
While targeting FXR holds promise , 6 - ethyl - CDCA known as obeticholic acid , the first in class of FXR ligands , causes side effects , partially because the lack of selectivity toward GPBAR1 , a putative itching receptor . - pubmed
Here , we describe the 3 - deoxy-6 - ethyl derivative of CDCA , BAR704 , as a highly selective steroidal FXR agonist . - pubmed
MAIN RESULTS : In transactivation assay BAR704 activated FXR with and EC50 of 967 nM while exerted no agonistic activity on other receptors including GPBAR1 . - pubmed
CONCLUSIONS AND APPLICATIONS : BAR704 is a selective FXR agonist that reduces liver fibrosis by interfering with the TGFbeta - SMAD3 pathway in HSCs . - pubmed
Herein , we detected an SSR embedded in the promoter region of the CYP82Y1 gene , which was found to be the first committed - step enzyme in the noscapine biosynthesis pathway , using the MISA program . - pubmed
METHODS : Following a legislative change that allowed EMTs to administer naloxone in one rural state , we evaluated an EMT training program by examining EMTs ' opioid overdose knowledge and attitudes before and after the training . - pubmed
The controlled pyrolysis of ZIF-67 can lead to the formation of Co nanoparticles encapsulated in nitrogen - doped porous carbon ( Co NC ) , which subsequently underwent galvanic replacement with Na2PdCl4 to form Co@Pd core - shell nanoparticles embedded in nitrogen - doped porous carbon ( Co@Pd NC ) . - pubmed
The Co@Pd NC exhibited outperformance in ORR and HER than commercial Pd / C , as manifested by more positive onset potential and larger diffusion - limited current density in ORR tests , as well as a small overpotential to drive a current density of 10 mA cm-2 , and much lower Tafel slope in HER tests . - pubmed
Estimation of pancreatic R2 * for iron overload assessment in the presence of fat : a comparison of different approaches . - pubmed
Altitude training was first used to prepare for competition at altitude , and it still is today ; however , more often now , elite athletes embark on a series of altitude training camps to try to improve sea - level performance . - pubmed
Furthermore , liquid chromatography - mass spectrometry ( LC - MS ) analysis of ethyl acetate extract identified 80 different compounds based on mass - to - charge ratio , database difference , resolution of mass spectrum and so on . - pubmed
Herein , a rigid squarate - based metal - organic framework ( MOF ) , having a perfect pore size ( 4.1 A x 4.3 A ) comparable with the kinetic diameter of Xe ( 4.047 A ) as well as pore surface decorated with very polar hydroxyl groups , is able to effectively discriminate Xe atoms , affording a record - high Xe / Kr selectivity . - pubmed
The MOF exhibits the highest Xe Henry coefficient ( 192.1 mmol / g / bar ) and Xe / Kr Henry selectivity ( 54.1 ) among all state - of - the - art adsorbents reported so far . - pubmed
However , enzymes that target diterpenoid compounds and show specific activity for side - chain glucosyl groups are rare . - pubmed
SPBGL1 displayed high specificity toward sophorose , and activity toward stevioside , but not rebaudioside A. - pubmed
SPBGL1 also operated at high substrate concentrations , such as in 50 % crude steviol glycoside extract . - pubmed
Glucose liberated from stevioside was easy to quantify using the glucose oxidase method , allowing the stevioside content to be determined . - pubmed
Simply linear regression analysis demonstrated a positive correlation between serum preptin and gender , body mass index ( BMI ) , blood urea nitrogen , creatinine , ACR , and a negative correlation between serum preptin and glomerular filtration rate , metformin , acarbose treatment . - pubmed
A label - free immunosensor based on PtPd NCs@MoS2 nanoenzymes for hepatitis B surface antigen detection . - pubmed
We introduced new nanoenzymes to a label - free electrochemical immunosensor for Hepatitis B surface antigen ( HBs Ag ) detection . - pubmed
The prepared nanoenzymes exhibited enhanced peroxidase - like activity than separate MoS2 and PtPd NCs . - pubmed
PtPd NCs@MoS2 were used for sensitive detection of HBs Ag , which is ascribed to their superior peroxidase activity , good conductivity and high specific surface area and synergistic amplification for current signals . - pubmed
Glibenclamide ameliorates transplant - induced arteriosclerosis and inhibits macrophage migration and MCP-1 expression . - pubmed
We used hematoxylin and eosin ( HE ) and Elastic Van Gieson ( EVG ) staining for histological assessment , and qRT - PCR and ELISA to measure mRNA and protein levels . - pubmed
Morphologically , glibenclamide reduced inflammatory cell accumulation and collagen deposition in the aortas . - pubmed
Furthermore , glibenclamide inhibited macrophage expression and secretion of inflammatory factors in vitro through suppressing activation of the nuclear factor - kappaB ( NF - kappaB ) pathway and MCP-1 production . - pubmed
CONCLUSION : Glibenclamide protected against aorta transplantation - induced arteriosclerosis by reducing inflammatory factors in vivo and inhibited macrophage migration and MCP-1 production in vitro . - pubmed
Elevated serum 17 - hydroxyprogesterone ( 17 - OHP ) and CYP21 gene molecular analysis confirmed the diagnosis . - pubmed
H2S mediates apoptosis in response to inflammation through PI3K / Akt / NFkappaB signaling pathway . - pubmed
Moreover , exogenous H2S rescued LPS - induced cystathionine gamma - lyase ( CSE ) inhibition and cystathionine beta - synthase ( CBS ) synthesis . - pubmed
Interestingly , in cells undergoing inflammation - induced apoptosis , H2S activated the PI3K / Akt and NFkappaB signal pathways both tested concentrations . - pubmed
CONCLUSIONS : H2S regulates LPS - induced inflammation and apoptosis by activating the PI3K / Akt / NFkappaB signaling pathway . - pubmed
FTIR showed that O - H bond was destroyed after torrefaction for both FRP and SP . - pubmed
18F - FDG PET / CT is limited to aggressive forms and PET imaging using 68Ga - somatostatin analogs appears suboptimal . - pubmed
Using a set of compounds ( new derivatives , known products and GABAA subtype receptor ligands from our library ) we identify the amino acids at the alpha+/gamma- interface , which could be involved in the agonist or antagonist profile , using the ' Proximity Frequencies ' , namely the frequencies with which a ligand intercepts two or more binding - site amino acids during the molecular dynamic simulation . - pubmed
Severity and time course of these phenomena suggest that early institution of direct ( Trasylol ) or indirect ( heparins , cortocosteroids ) proteinase inhibition , and if necessary a replacement of the lost factors , should be considered . - pubmed
[ C - erbB-2 oncogene amplification in breast cancer in correlation to steroid and epidermal growth factor receptor ] . - pubmed
Lipid peroxidation parameters , such as malonic dialdehyde and antioxidant defense ( superoxide dismutase and catalase activities ) were studied in healthy individuals , miners of different occupations , working at mines of different altitudes . - pubmed
Loss of thrombin - induced Ca2 + mobilization in a subpopulation of platelets during storage . - pubmed
The maximal concentration of [ Ca2 +] i after stimulation with 0.5 U of thrombin / ml decreased from 544 + /- 58 nM ( mean + /- SEM , n = 6 ) on day 0 , to 276 + /- 9 nM on day 3 and to 203 + /- 23 nM on day 6 . - pubmed
The study shows that during platelet storage a decrease in the rise in [ Ca2 +] i upon thrombin stimulation occurs . - pubmed
ADP ribosylation and G protein regulation in the thyroid . - pubmed
Group I received no antiplatelet agents ( control ) ; group II received U-63,557A , a specific thromboxane synthetase inhibitor ( TSI ) ; group III received aspirin ; and group IV received aspirin and TSI . - pubmed
The fact that platelets , PMN leukocytes , and MN leukocytes concentrate ascorbic acid suggests that vitamin C has an important role in their physiological functions . - pubmed
They also found that MN leukocytes , in contrast , do not show any such relationship ; however , MN leukocytes maintain the highest levels of ascorbic acid and play a very important function in immunocompetence . - pubmed
We have found that with a limited number of subjects , ascorbic acid content of MN and PMN leukocytes correlates positively with plasma ascorbic acid , but there was no correlation between platelets and plasma ascorbic acid ( unpublished results ) . - pubmed
These effects of PAF on LDL and lipid metabolism could be related to perturbations in membrane structure characteristics , leading to a delay in LDL delivery to lysosomes , and to modification of the activity of some key enzymes of lipid metabolism . - pubmed
LDH isoenzymes were studied using electrophoresis in polyacrylamide gel . - pubmed
Thyrotropin- and prostaglandin E2 - responsive adenyl cyclase in thyroid plasma membranes . - pubmed
[ Effect of carbonic anhydrase inhibitor , benzanilamide , on activity of carbonic anhidrase in the ciliary body and intraocular pressure of glaucomatous eyes ] . - pubmed
Studies on the effect of thyroxine on in vivo insulin secretion as modified by hypophysectomy . - pubmed
The finding that alprazolam was effective in the treatment of panic disorder is of interest as this diagnostic category is usually treated with tricyclic antidepressants or MAO inhibitors . - pubmed
Lithium chloride lacked a direct effect on tyrosine hydroxylase ( TH ) , monoamine oxidase ( MAO ) , or catechol - O - methyltransferase ( COMT ) in concentrations up to 2 mM. - pubmed
[ Urinary kallikrein excretion , renin - angiotensin - aldosterone system and urinary electrolytes excretion in hypertensive outpatients ] . - pubmed
Treatment with both oxygen and subcutaneous sumatriptan reduced the CGRP level to normal , while opiate administration did not alter the peptide levels . - pubmed
Zinc is a cofactor for alcohol dehydrogenase , the ethanol metabolizing enzyme . - pubmed
Adverse effects of Plachitin particles for arterial chemoembolization were epigastralgia , nausea , fever , and elevation of serum aspartate aminotransferase ( AST ) and alanine aminotransferase ( ALT ) levels . - pubmed
Monoclonal antibodies ( MoAbs ) specific for glycyrrhetic acid ( GA ) were prepared and characterized . - pubmed
Obtained MoAbs ( AGA-1 , AGA-3 , and AGA-6 ) reacted with GA dose - dependently , but not with glycyrrhizin ( GL ) , carbenoxolone , and steroids . - pubmed
5 - Lipoxygenase has been recognized to be an important enzyme that catalyzes the first step in leukotriene production . - pubmed
Among the synthesized phospholipids examined , 1 - oleoyl-2 - docosahexaenoyl - sn - glycero-3 - phosphocholine ( 1 - oleoyl-2 - DHA - PC ) was found to be the most potent inhibitor of 5 - lipoxygenase . - pubmed
Lineweaver - Burk plot analysis showed that the inhibition of 5 - lipoxygenase by 1 - oleoyl-2 - DHA - PC was non - competitive . - pubmed
The inhibition by this synthesized phospholipid was very specific to 5 - lipoxygenase ; that is , it did not extend to fatty acid cyclooxygenase , 12 - lipoxygenase or 15 - lipoxygenase . - pubmed
These results suggest that endogenously existing DHA - containing phospholipids may affect 5 - lipoxygenase activity and thus control leukotriene biosynthesis in vivo . - pubmed
They were incubated for isolation in Hanks ' balanced salt solution with 0.35 % collagenase and Earle 's solution with 1 mM EDTA . - pubmed
NG - nitro - D - arginine methyl ester ( D - NAME , a less active inhibitor of NO synthase ) showed no effects in the above behavioural tasks . - pubmed
Quantitative DNase I footprint titration experiments reveal that ImPyPy - gamma - PyPyPy - beta - Dp binds all eight 5'-TG(A , T)(3)-3 ' target sites with only a 12 - fold difference in the equilibrium association constants between the strongest site , 5'-TGTTT-3 ' ( Ka = 2.1 x 10(8 ) M-1 ) , and the weakest site , 5'-TGAAT-3 ' ( Ka = 1.8 x 10(7 ) M-1 ) ( 10 mM Tris . - pubmed
INTERVENTIONS : Each family member completed a questionnaire and underwent expiratory flow measurement , skin prick tests for allergies , methacholine tests and measurement of total serum IgE level and blood eosinophil count . - pubmed
RESULTS : The group of families with a member with asthma had an increased prevalence of atopy and AHR , lower PC20 methacholine and higher serum IgE levels in comparison with controls , even when the first identified family members with asthma and their matched controls were not included . - pubmed
There was a significant correlation between PC20 methacholine level and atopic index , between blood eosinophil count and serum IgE level , and between atopic index and serum IgE level , for all groups . - pubmed
Single nucleotide modulation of uridine to pseudouridine rearrangement in transfer RNA catalyzed by pseudouridine synthase I. - pubmed
coli pseudouridine synthase I ( PSUI ) catalyzes the rearrangement of uridine residues in positions 38 , 39 and 40 of tRNA transcripts to pseudouridine . - pubmed
Receptor expression was observed in cells that had earlier been shown to express cGMP - dependent kinase , but not the cystic fibrosis transmembrane regulator , a known downstream target of cGMP / G - kinase , which suggests that GCC/ cGMP could regulate additional cellular signal transduction machinery . - pubmed
In addition to histamine , MC contain many other angiogenic factors and a variety of cytokines , growth factors , and proteolytic enzymes implicated in tissue remodeling , and normal as well as tumor - associated neoangiogenesis . - pubmed
Chronic administration of PTU or PTU plus T4 inhibited the 3 beta - hydroxysteroid dehydrogenase , 21 beta - hydroxylase , and 11 beta - hydroxylase activities . - pubmed
In cultured cells transfected with the human TSH receptor , hCG increases generation of cAMP . - pubmed
Molecular variants of hCG with increased thyrotropic potency include basic molecules with reduced sialic acid content , truncated molecules lacking the C - terminal tail , or molecules in which the 47 - 48 peptide bond in the beta - subunit loop is nicked . - pubmed
In normal pregnancy , when hCG levels are highest at 10 to 12 weeks gestation , there is suppression of serum TSH levels , presumably due to slight increases in free thyroxine ( T4 ) concentration . - pubmed
Furthermore , an increased toxicity was observed when Al was concurrently administered with CA , SA , VA , PCA , and HY . - pubmed
Inversely , significant cytoprotective effects were noted with EDTA , HA , CFA , and PA . - pubmed
The [ rho(0 ) ] mutants and antimycin - treated cells differ from the wild type in PPM composition and invertase , acid phosphatase , and glucoamylase activities . - pubmed
The reduced flocculation of cells grown in the presence of antimycin , under anaerobiosis , or carrying a [ rho(0 ) ] mutation may be the consequence of alterations of PPM structures which are the ligands of lectins , both involved in this cell - cell recognition phenomenon . - pubmed
dose - dependently enhanced ( 9/26 neurons ) or suppressed ( 8/26 ) activity , and the 5 - HT1A antagonist ( + ) WAY-100135 often attenuated these responses . - pubmed
CONCLUSION : The lack of correlation between total GST and protease activity for most of the enzymes investigated and alanyl AP levels in perfusate samples could be related to differences in cellular localisation , suggesting that assays of alanyl AP may give complimentary biochemical information relating to kidney tissue damage . - pubmed
Here we report more details on one of the genes identified , a hemN - like gene ( now called hemN ( 1 ) ) whose product exhibits significant similarity to oxygen - independent coproporphyrinogen III dehydrogenases involved in heme biosynthesis in facultatively anaerobic bacteria . - pubmed
The hemN ( 2 ) gene maps outside of the symbiotic gene region ; it is located 1.5 kb upstream of nirK , the gene for a Cu - containing nitrite reductase . - pubmed
The two deduced HemN proteins are similar in size ( 445 and 450 amino acids for HemN(1 ) and HemN(2 ) , respectively ) and share 53 % identical ( 68 % similar ) amino acids . - pubmed
Moreover , these mutants lacked several c - type cytochromes which are normally detectable by heme staining of proteins from anaerobically grown wild - type cells . - pubmed
The results are discussed in terms of energization of SE transporters for retrieval of leaking sucrose , K + and amino acids , as one of the unknown roles of ATP found in SEs . - pubmed
The function of the PPase could be related to phloem sucrose metabolism in support of ATP - requiring processes . - pubmed
Rapid degradation of auxin / indoleacetic acid proteins requires conserved amino acids of domain II and is proteasome dependent . - pubmed
Auxin rapidly induces auxin / indoleacetic acid ( Aux / IAA ) transcription . - pubmed
The C - terminal 109 amino acids did not affect LUC half - life . - pubmed
New data reveal that insulin may regulate this process in part by promoting membrane trafficking of intracellular fatty acid transporters FATP1 and FATP4 to the plasma membrane . - pubmed
Exonic splicing enhancer - dependent splicing of the gonadotropin - releasing hormone premessenger ribonucleic acid is mediated by tra2alpha , a 40 - kilodalton serine / arginine - rich protein . - pubmed
In an earlier study , we found that excision of the first intron ( intron A ) from the rat GnRH primary transcript is attenuated in non - GnRH - producing cells . - pubmed
In the presence of an ESE in exon 4 ( ESE4 ) , an addition of GT1 NE further increased the excision rate of intron A , whereas the addition of KK1 ( a non - GnRH - producing cell ) NE decreased it . - pubmed
To define the GnRH neuron - specific splicing activity , GT1 NE was fractionated by ultracentrifugation and ammonium sulfate precipitation . - pubmed
A 50 - 90 % ammonium sulfate pellet ( ASP50 - 90 ) fraction was further precipitated with 20 mM MgCl ( 2 ) to isolate a serine / arginine - rich ( SR ) protein fraction . - pubmed
Among the ASP fractions , ASP40 - 50 significantly increased the excision rate of intron A in the presence of HeLa NE or SR protein - rich fraction . - pubmed
These results imply that Tra2alpha and a cofactor protein in the ASP40 - 50 fraction are involved in mediating the GnRH neuron - specific excision of intron A from the GnRH primary transcript . - pubmed
OBJECTIVES : To study the relationship between serum levels of hyaluronic acid ( HA ) , type III procollagen ( PCIII ) , laminin ( LN ) , type IV collagen ( IV - C ) and hepatic fibrosis and to determine their value in clinical practice . - pubmed
FINDINGS : After a mean follow - up of 5.1 years , fluvastatin lowered LDL cholesterol concentrations by 32 % . - pubmed
Sodium dodecyl sulfate at pH 6.0 - 6.3 cleaves the DNA of isolated gelable nuclei from the residual protein of these nuclei over a period of 2 to 3 hours . - pubmed
BACKGROUND : Tacrolimus is an immunosuppressive drug that is a substrate of cytochrome P450 3A ( CYP3A ) enzymes and P - glycoprotein ( P - gp ) . - pubmed
Corticosteroids induce both CYP3A and P - gp activity . - pubmed
After treated with genistein , BMD and the serum level of ALP , ACP , osteocalcin increased significantly , while the serum level of IL-1beta and TNF - alpha decreased . - pubmed
The protein has a fold similar to that of disulfide reductases and consists of the FAD - binding , NAD - binding , and C - terminal domains . - pubmed
Compared to homologous flavoenzymes , the reductase component of biphenyl dioxygenase ( BphA4 ) and apoptosis - inducing factor , Pdr lacks one of the arginine residues that compensates partially for the negative charge on the pyrophosphate of FAD . - pubmed
The aromatic side - chain of the " gatekeeper " Tyr159 is in the " out " conformation and leaves the nicotinamide - binding site of Pdr completely open . - pubmed
A structural comparison of Pdr with homologous flavoproteins indicates that an open and accessible nicotinamide - binding site , the presence of an acidic residue in the middle part of the NAD - binding channel that binds the nicotinamide ribose , and multiple positively charged arginine residues surrounding the entrance of the NAD - binding channel are the special structural elements that assist tighter and more specific binding of the oxidized pyridine nucleotide by the BphA4 - like flavoproteins . - pubmed
Here , it is reported that deletion of ppoB ( DeltappoB ) reduced production of the oleic - acid - derived oxylipin psiBbeta and increased the ratio of asexual to sexual spore development . - pubmed
Generation of the triple mutant Delta ppoA Delta ppoB Delta ppoC resulted in a strain deficient in producing oleic - and linoleic - acid - derived 8 '- hydroxy psi factor and caused increased and mis - scheduled activation of sexual development . - pubmed
The purpose of this study was to investigate the localization and distribution of ferrofluid microsphere of human serum albumin methotrexate ( FM - HSA - MTX ) carriers in the brain and to explore the magnetic targeting chemotherapy for malignant brain tumor . - pubmed
Synthesized FM - HSA - MTX carriers ( MTX 25 mg / kg ) were injected into the systemic circulation via the caudal vein ( magnetic targeting group , n = 30 ) . - pubmed
The non - magnetic targeting group ( n = 30 ) was administered with FM - HSA - MTX without external magnetic field , meanwhile the control group ( n = 30 ) was treated with MTX and a magnetic field . - pubmed
We measured urinary iodine ( UI ) , thyroid - stimulating hormone ( TSH ) , and total thyroxine ( TT4 ) concentrations and thyroid gland volume ( by ultrasound ) . - pubmed
Enhanced cystatin C and lysosomal protease expression following 6 - hydroxydopamine exposure . - pubmed
Following 6 - OHDA exposure , the caspase 3 - like inhibitor Ac - DEVD - CHO significantly decreased 6 - OHDA induced cell death . - pubmed
In addition , alterations in expression of the lysosomal cysteine and aspartic proteases , cathepsin B ( CB ) and cathepsin D ( CD ) and the endogenous cysteine protease inhibitor cystatin C were observed utilizing immunocytochemical analysis at 24 , 48 , and 72 h following 6 - OHDA exposure . - pubmed
Moreover , Western blot analysis confirmed a significant increase in protein expression for CB and CD at 72 h and a temporal and concentration dependent increase in cystatin C in response to 6 - OHDA . - pubmed
Cells treated with pepstatin A , an inhibitor for CD , showed a significant decrease in cell death , however , CA-074ME , a specific inhibitor for CB , failed to protect cells from 6 - OHDA induced cell death . - pubmed
Thus , these results suggest that apoptosis induced by 6 - OHDA exposure is mediated in part through caspase 3 activation and lysosomal protease CD . - pubmed
Effects of aprotinin on plasma coagulation kinetics determined by thrombelastography : role of Factor XI . - pubmed
Thrombelastography has been utilized to monitor coagulation in these settings , and aprotinin delays clot initiation , presumably by inhibiting kallikrein ; however , aprotinin also inhibits Factor XI ( FXI ) , a contact system protein . - pubmed
Thus , it was hypothesized that celite - activated thrombelastography coagulation kin - etics would be decreased via aprotinin - mediated FXI inhibition . - pubmed
Exposure of celite - activated , prekallikrein - deficient plasma to aprotinin 0 , 200 , 400 or 800 KIU / ml resulted in R values of 366 + /- 15 , 630 + /- 64 , 698 + /- 46 and 850 + /- 47 s , respectively , which were significantly different from one another ( P & lt ; 0.05 ) . - pubmed
There were no significant differences in R values between tissue factor - activated , prekallikrein - deficient plasma samples with 0 or 800 KIU / ml aprotinin . - pubmed
CONCLUSIONS : These data support a role for the inhibition of FXI as the mechanism for aprotinin - mediated delayed contact system clot initiation determined by thrombelastography . - pubmed
We examined the mechanism of Al - induced citrate release by studying the relationship between citrate release and expression of the mitochondrial citrate synthase ( mCS ) gene in three leguminous trees . - pubmed
Lanthanum did not alter the expression level of mCS . - pubmed
We studied the correlation between serum interleukin-2 ( IL-2 ) and tumor necrosis factor - alpha ( TNF - alpha ) levels and the response to 89SrCl2 therapy . - pubmed
After treatment with 89SrCl2 , IL-2 levels increased and TNF - alpha levels decreased , with maximal changes at the fourth month after therapy . - pubmed
After comparing the serum levels of IL-2 and TNF - alpha between responders and nonresponders , we found that these variables did not differ before 89SrCl2 therapy but differed significantly ( P & lt ; 0.05 ) after therapy . - pubmed
Further studies are needed to elucidate the roles of IL-2 and TNF - alpha in the response to 89SrCl2 therapy and to evaluate their usefulness as indicators of 89SrCl2 efficacy . - pubmed
Responses of neurons containing VGLUT3 / nNOS - cGMP in the rVLM to cardiac stimulation . - pubmed
Colocalization of neuronal nitric oxide synthase , soluble guanylyl cyclase and c - Fos or vesicular glutamate transporter 3 was noted following bradykinin stimulation . - pubmed
Immediately following exposure , the cytosol accounted for 40 % of the body burden in insects from both sites ; however , 89 + /- 2 % of the cytosolic Cd was associated with metallothionein - like proteins ( MTLP ) in insects from the contaminated site , compared to 60 + /- 0 % in insects from the reference site . - pubmed
Results suggested that differences in exposure history had no influence on the bioaccumulation of Cd , but did affect the concentrations of Cd bound to MTLP during Cd exposure in these insects . - pubmed
This suggests that , at high phosphate doses , activation of the proton pump is induced by the longer lasting proton influx acidifying the cytoplasm . - pubmed
The increased H (+ ) efflux due to the proton pump could partially compensate protons taken up via the proton - phosphate cotransport system . - pubmed
CONTEXT : Despite the importance of dihydrotestosterone in androgen action , polymorphisms in the genes for the two isoforms of 5alpha - reductase ( SRD5A1 and SRD5A2 ) have not been evaluated as risk factors for polycystic ovary syndrome ( PCOS ) . - pubmed
The Leu allele of the Val89Leu variant in SRD5A2 was associated with protection against PCOS ; this allele is known to modestly reduce 5alpha - reductase activity . - pubmed
Results from the present study suggest , for the first time , that PLC attenuates forebrain ischaemia - induced neuronal injury , oxidative stress and energy depletion in the hippocampal CA1 region . - pubmed
A highly efficient synthesis of potent and selective butyrolactam inhibitors of 11beta - HSD1 . - pubmed
A convergent synthesis of structurally novel butyrolactam 11beta - HSD1 inhibitors is described . - pubmed
Using a yeast two - hybrid screen , NECAB2 , a neuronal calcium binding protein , was identified as a binding partner for the adenosine A ( 2A ) receptor ( A ( 2A ) R ) interacting with its C - terminal domain . - pubmed
Sequence analysis of the tagged Tn917 - interrupted genes in these mutants indicated the participation in pathogenesis of the transcriptional regulatory proteins homologous to GidA and MerR ; the metabolic enzymes asparagine synthetase A and alpha - acetolactate synthase ; the ABC transport system of glutamine and a calcium - transporting ATPase ; the dltA locus involved in alanylation of teichoic acids ; and hypothetical proteins containing EAL and Eis domains , among others . - pubmed
The anti - VEGF antibody bevacizumab enhances the response rate and prolongs disease control when added to interferon - alpha . - pubmed
5 - Nitrobenzo [ b ] thiophene-2 - carboxylic acid 1 is converted to the corresponding hydrazide 2 by the reaction with hydrazine hydrate and then to the azide 3 with nitrous acid , followed by thermal rearrangement , cooling , and trapping in one pot reaction . - pubmed
Nitrogenase model complexes [ Cp * Fe ( mu - SR ( 1 )) 2 ( mu - eta ( 2 )- R ( 2 ) N = NH ) FeCp * ] ( R(1 ) = Me , Et ; R(2 ) = Me , Ph ; Cp * = eta ( 5 )- C5Me5 ): synthesis , structure , and catalytic N - N bond cleavage of hydrazines on diiron centers . - pubmed
The reactions of [ Cp * Fe ( mu - SR1 ) 3FeCp * ] ( Cp * = eta5 - C5Me5 ; R1 = Et , Me ) with 1.5 equiv R2NHNH2 ( R2 = Ph , Me ) give the mu - eta2 - diazene diiron thiolate - bridged complexes [ Cp * Fe ( mu - SR1 ) 2 ( mu - eta2 - R2N NH ) FeCp * ] , along with the formation of PhNH2 and NH3 . - pubmed
Aortic inflammation , as assessed by hybrid FDG - PET / CT imaging , is associated with enhanced aortic stiffness in addition to concurrent calcification . - pubmed
PURPOSE : To analyse the relationship between : ( i ) aortic pulse wave velocity ( PWV ) , an index of aortic stiffness with strong prognostic significance , and ( ii ) aortic calcification and inflammation , which were quantified by hybrid imaging with X - ray computed tomography ( CT ) and ( 18 ) F - fluorodeoxyglucose ( FDG ) positron emission tomography ( PET ) . - pubmed
CONCLUSION : Aortic inflammation , assessed by hybrid FDG - PET / CT imaging , is associated with an enhanced aortic stiffness , in addition to the concurrent impact of calcifications . - pubmed
Expression of the P2X7 receptor increases the Ca2 + content of the endoplasmic reticulum , activates NFATc1 , and protects from apoptosis . - pubmed
Here we show that P2X ( 7 ) transfection increased the ability of the endoplasmic reticulum to accumulate , store , and release Ca ( 2 + ) . - pubmed
This caused a larger agonist - stimulated increase in cytosol and mitochondrial Ca ( 2 + ) in P2X ( 7 ) transfectants than in mock transfected cells . - pubmed
P2X ( 7 ) transfectants survived and even proliferated in serum - free conditions and were resistant to apoptosis triggered by ceramide , staurosporin , or intracellular Zn ( 2 + ) chelation . - pubmed
Whole - body fluorine-18 fluorodeoxyglucose positron emission tomography and CT ( FDG - PET / CT ) performed 15 days after right nephrectomy showed intense FDG uptake in the aforementioned lesions ( lung and pleural nodules , mediastinal lymph nodes ) . - pubmed
FDG - PET / CT was considered useful for evaluating distant metastases and thus portending the aggressive nature of sarcomatoid RCC . - pubmed
RESULTS : Neocytolysis ensued that most evident from a rapid rise in serum ferritin as the iron from young RBC was transferred back to stores . - pubmed
The contents of trehalose constitutively accumulated in the TPS1- and TPS2 - overexpressing triple deletion strains were higher than that in the original triple deletion strain . - pubmed
High trehalose accumulation and growth activity were observed in the TPS2 - overexpressing triple deletion strain after ethanol stress induction . - pubmed
Based on these results , we have explored the molecular mechanisms underlying circadian oscillation and phase response to light of the master clock located in the dorsomedial subdivision of the suprachiasmatic nucleus ( SCN ) and found various proteins closely related to phase response such as BIT / SHPS-1 and those of circadian oscillation , some of which are involved in protein - tyrosine phosphorylation . - pubmed
On the other hand , we have presented several lines of evidence that the ventrolateral subdivision of the SCN includes not only the control center of energy supply to the brain , but also that of homeostasis such as blood glucose , blood pressure , water balance , and body temperature . - pubmed
We have also shown that besides these functions , the latter subdivision is involved in the regulations of hormone secretions such as insulin , glucagon , corticosterone and vasopressin . - pubmed
Results from molecular docking and MD simulations demonstrate that nine PPIs adopt two different conformations ( extended and U - bend structures ) at the binding sites and position themselves far above the heme of 2C9 . - pubmed
Interestingly , at the binding sites of all PPI - CYP2C9 complexes except for Lan / CYP2C9 , there are hydrogen - bonding networks made of PPIs , water molecules , and some residues of 2C9 . - pubmed
However , in the case of Lan/2C9 , the hydrophobic interactions are more important than the electrostatic interactions for stabilizing the binding site . - pubmed
Bax regulates production of superoxide in both apoptotic and nonapoptotic neurons : role of caspases . - pubmed
A Bax- and , apparently , mitochondria - dependent increase in superoxide ( O ( 2 ) ( - ) ) and other reactive oxygen species ( ROS ) occurs in apoptotic superior cervical ganglion ( SCG ) and cerebellar granule ( CG ) neurons . - pubmed
We used the O ( 2 ) ( -)-sensitive dye MitoSOX to monitor O ( 2 ) ( - ) in neurons expressing different levels of Bax and mitochondrial superoxide dismutase ( SOD2 ) . - pubmed
Basal and apoptotic O ( 2 ) ( - ) levels in both SCG and CG neurons were reduced in SOD2 wild - type ( WT ) cells having lower Bax concentrations . - pubmed
A caspase inhibitor inhibited O ( 2 ) ( - ) in both apoptotic and nonapoptotic SCG neurons . - pubmed
O ( 2 ) ( - ) production increased when WT , but not Bax - null , SCG neurons were permeabilized and treated with active caspase 3 . - pubmed
O ( 2 ) ( - ) levels in nonapoptotic caspase 3 - null SCG neurons were lower than in WT cells but not as low as in caspase inhibitor - treated cells . - pubmed
These data indicate that Bax lies upstream of most O ( 2 ) ( - ) produced in neurons , that caspase 3 is required for increased O ( 2 ) ( - ) production during neuronal apoptosis , that caspase 3 is partially involved in O ( 2 ) ( - ) production in nonapoptotic neurons , and that other caspases may also be involved in Bax - dependent O ( 2 ) ( - ) production in nonapoptotic cells . - pubmed
Influence of CYP2C8 * 13 and CYP2C8 * 14 alleles on amiodarone N - deethylation . - pubmed
Effects of an N - type calcium antagonist on angiotensin II - renin feedback . - pubmed
RESULTS : Valsartan increased the plasma angiotensin II ( Ang II ) level in a dose - dependent manner . - pubmed
Cilnidipine suppressed the increase in plasma renin activity and plasma Ang II levels induced by valsartan , but amlodipine did not . - pubmed
CONCLUSIONS : The results of this study suggest that the suppressive effect of cilnidipine on the valsartan - induced increase in RAS activity can be partly explained by its sympatholytic action mediated by N - type calcium channel blockade , and that combined administration of cilnidipine and valsartan might provide a synergistic therapeutic effect . - pubmed
To further explore the role of dcEFs in regulating endothelial cells , we analyzed the endothelial cell proliferation and secretion of nitric oxide ( NO ) , endothelin-1 ( ET-1 ) in response to dcEFs of physiological strength . - pubmed
The results also showed dcEFs did not affect cell proliferation , but affected the productions of NO and ET-1 . - pubmed
When COCs were cultured with FSH , Fshr mRNA expression was immediately decreased within 5 h , whereas Ptger2 , Ptger4 , and Ptgs2 expression levels were significantly increased in cumulus cells in the culture containing FSH for 5 or 10 h. The PTGS2 inhibitor NS398 significantly suppressed not only PGE2 secretion at any culture time point but also Areg , Ereg , and Tace / Adam17 expression in cumulus cells at 10 and 20 h but not at 1 or 5 h. During the early culture period , phosphorylation of MAPK3 and MAPK1 ( MAPK3 / 1 ) was not affected by NS398 ; however , at 10 and 20 h , phosphorylation was suppressed by the drug . - pubmed
Furthermore , down - regulations of MAPK3 / 1 phosphorylation and expression of the target genes by NS398 was overcome by the addition of either PGE2 or EGF . - pubmed
FSH - induced cumulus expansion and meiotic progression to the MII stage were also suppressed by NS398 , whereas these effects were also overcome by addition of either PGE2 or EGF . - pubmed
More trials with carefully designed outcome measures are needed , especially with other modern combinations , including mTOR inhibitors and/or belatacept . - pubmed
Activation of Rice nicotianamine synthase 2 ( OsNAS2 ) enhances iron availability for biofortification . - pubmed
Activation of iron deficiency - inducible OsNAS2 resulted in a rise in Fe content ( 3.0 - fold ) in mature seeds . - pubmed
One possible cause of NO deficiency is increased levels of endogenous NO synthase inhibitors , in particular asymmetric dimethylarginine ( ADMA ) . - pubmed
At the end of the procedure , lung tissue levels of thiobarbituric acid reactive substances ( TBARS ) , reduced glutathione ( GSH ) , superoxide dismutase ( SOD ) , and catalase ( CAT ) were determined . - pubmed
The most frequent epidermal growth factor receptor mutation we detected was a deletion in exon 19 ( 60.3 % , 38 patients ) , followed by an L858R amino acid substitution in exon 21 ( 27 % , 17 patients ) . - pubmed
On multivariate analysis using the Cox proportional hazards model , the presence of HLN metastasis ( P = 0.002 ) , along with a large number ( & gt ; or = 4 ) of regional lymph node metastases ( P = 0.003 ) , and nonuse of oxaliplatin - based chemotherapy ( P = 0.005 ) were identified as independent risk factors for shorter survival . - pubmed
Blood biochemical markers measured at baseline and at the end of the study included : triglycerides ( TG ) , cholesterol , HDL - C , LDL - C , VLDL - C , ApoB100 , ApoAI , C - reactive protein ( CRP ) , and fibrinogen . - pubmed
Serum total cholesterol ( TC ) decreased significantly in the treatment groups compared with the control group ( P & lt ; 0.005 ) . - pubmed
The BRAF(V600E ) inhibitor vemurafenib was used to confirm targets in BRAF(V600E)-positive melanoma cell lines and in tumors from melanoma patients undergoing inhibitor treatment . - pubmed
The primary influence of gender was structure / geometry in bone growth and mechanical properties , whereas the mineral / matrix composition and hydroxyproline content of bone were influenced primarily by the oim collagen mutation . - pubmed
The concentrations of total PCBs in the 2,4 - D were in the range of 16.1 and 8023ngkg(-1 ) , and the WHO - TEQ values of the PCBs were between 0.057 and 108.3ng WHO - TEQkg(-1 ) , while total PCBs were between 1486 and 47342ngkg(-1 ) . - pubmed
It was found that Garsi ( GR ) ( IC50=4.9 + -0.2 mg / L by ABTS assay ) and Zaghwani ( ZG ) ( IC50=3.9 + -0.2 mg / L by ABTS assay ) varieties exhibited the highest antioxidant activity . - pubmed
Both diets decreased serum total cholesterol , LDL - cholesterol and HDL - cholesterol levels , but D1 had a greater effect on total cholesterol and LDL - cholesterol levels ( P< ; 0 001 and P< ; 0 05 , respectively ) . - pubmed
D1 increased colonic fermentative activity , as judged from the higher ( P< ; 0 001 ) breath hydrogen levels recorded . - pubmed
We administered the same amount of the fluorescent dye , Nile red , into the middle ear in two forms : loaded in PHEA polymersomes ( NP group ) or diluted in ethanol ( NR group ) . - pubmed
Although Abeta hypothesis prevails in the ethiology of Alzheimer ' s Disease ( AD ) , it has become increasingly evident that disturbances in cerebral glucose metabolism is an invariant pathophysiological feature of AD and may provide an ubiquituos mechanism underlying the pathogenesis of AD . - pubmed
In addition , paeoniflorin increased glutathione level and restored the activity of glyoxalase I to almost the control level . - pubmed
Additionally , the nitric oxide and nuclear respiratory factor 1 levels were significantly increased by paeoniflorin , suggesting that paeoniflorin may induce mitochondrial biogenesis . - pubmed
Paeoniflorin treatment decreased the levels of proinflammatory cytokines such as TNF - alpha and IL-6 . - pubmed
CONCLUSIONS : These findings indicate that paeoniflorin might exert its therapeutic effects via upregulation of glyoxalase system and mitochondrial function . - pubmed
In spite of the low response rate , IFN - alpha therapy has the advantage of having a limited duration and being effective even after therapy , as demonstrated by a much higher incidence of HBsAg clearance in responders to IFN - alpha than in naturally occurring inactive HBsAg carriers . - pubmed
RESULTS : We identified a de novo missense mutation in KCNB1 that encodes the KV 2.1 voltage - gated potassium channel . - pubmed
The percentage of patients meeting each criterion of the AHA was as follows : 46 % for abdominal obesity ; 44 % for hypertriglyceridemia or cholesterol - lowering medication use ; 26 % for low high - density lipoprotein cholesterol or being on a lipid - lowering medication ; 45 % for hypertension ; and 20 % for high fasting glucose or anti - diabetic medication use . - pubmed
Basal tears were collected using a microcapillary tube and assayed for concentration and activity of the secretory phospholipase A2 enzyme and concentration of the lipid aldehyde malondialdehyde . - pubmed
Secretory phospholipase A2 enzyme activity in tears was associated with higher levels of malondialdehyde ( R = 0.65 , p = 0.01 ) and shorter NISDT ( R = 0.84 , p = 0.001 ) . - pubmed
Early postinfarction ST2 levels and change over 24 weeks are related to infarct extent and remodeling to a similar extent as NT - proBNP and aldosterone , and ST2 may have a significant pathophysiological role in these postinfarction processes . - pubmed
Estrogen - related Receptor beta Reduces the Subnuclear Mobility of Estrogen Receptor alpha and Suppresses Estrogen - dependent Cellular Function . - pubmed
ERR has three subtypes ( alpha , beta , and gamma ) expressed in estrogen - sensitive organs , including ovary , breast , and brain . - pubmed
No endogenous ligands of ERRs have been identified , but these receptors share a common DNA element with ERalpha and control estrogen - mediated gene transcription . - pubmed
Recent evidence suggests a role of ERRs in estrogen - related pathophysiology , but the detailed mechanisms of ERR functions in estrogen - related tissues are unclear . - pubmed
Using live - cell imaging with fluorescent protein labeling , we found that only ERRbeta among the ERRs exhibits a punctate intranuclear pattern overlapping with ERalpha following 17beta - estradiol ( E2)-stimulation . - pubmed
These results provide strong evidence for a suppressive effect of ERRbeta on estrogen signaling through reduction of the intranuclear mobility of ERalpha . - pubmed
The findings further suggest a unique inhibitory role for ERRbeta in estrogen - dependent cellular function such as cancer cell proliferation . - pubmed
Genistein increases estrogen receptor beta expression in prostate cancer via reducing its promoter methylation . - pubmed
Genistein has protective effects against prostate cancer ( PCa ) but whether this protection involves an estrogen receptor ( ER ) beta dependent mechanism has yet to be elucidated . - pubmed
However , the ability of genistein to reverse ER - beta promoter hypermethylation in PCa has not been studied . - pubmed
In addition , there are great discrepancies among studies that examined the effect of genistein on ER - beta gene expression . - pubmed
We also examined the role of ER - beta in mediating the preventive action of genistein . - pubmed
Genistein induced phosphorylation , nuclear translocation and transcriptional activity of ER - beta in all three PCa cell lines . - pubmed
In conclusion , genistein and ER - beta act together to prevent PCa cell proliferation ; genistein increases ER - beta levels via reducing its promoter methylation and ER - beta , in turn , mediates the preventive action of genistein . - pubmed
Arnebin-1 promotes angiogenesis by inducing eNOS , VEGF and HIF-1alpha expression through the PI3K - dependent pathway . - pubmed
In this study , in an aim to elucidate these mechanisms of action of arnebin-1 , we investigated the effects of arnebin-1 on the VEGF receptor 2 ( VEGFR2 ) and the phosphoinositide 3 - kinase ( PI3K)-dependent signaling pathways in HUVECs treated with VEGF by western blot analysis . - pubmed
We found that the activation of the VEGFR2 signaling pathway induced by VEGF was enhanced by arnebin-1 . - pubmed
Arnebin-1 promoted endothelial cell proliferation , migration and tube formation through the PI3K - dependent pathway . - pubmed
Moreover , Arnebin-1 significantly increased the eNOS , VEGF and HIF-1alpha expression levels in the HUVECs and accelerated the healing of diabetic wounds through the PI3K - dependent signaling pathway . - pubmed
The molecular mechanism of polygalasaponin F - mediated decreases in TNFalpha : emphasizing the role of the TLR4 - PI3K / AKT - NF - kappaB pathway . - pubmed
The results indicated that inhibition of toll - like receptor ( TLR ) 4 ( CLI-095 1 mug / ml ) , phosphatidylinositol 3 - kinase ( PI3 K ) ( Ly294002 10 muM ) or IkappaBalpha phosphorylation ( Bay11 - 7082 10 muM ) completely prevents the release of TNFalpha induced by LPS without affecting cell viability and attenuated the nuclear translocation of p65 stimulated by LPS . - pubmed
The crystal and mol - ecular structures of the title salt , C8H8N3S2 ( + ) Cl (- ) , ( I ) , and salt hydrate , C8H7ClN3S2 ( + ) Cl (- ) H2O , ( II ) , are described . - pubmed
The common feature of the crystal packing in ( I ) and ( II ) is the formation of charge - assisted amino - N - H Cl (- ) hydrogen bonds , leading to helical chains in ( I ) and zigzag chains in ( II ) . - pubmed
In ( II ) , the chains are linked into a layer by charge - assisted water - O - H Cl (- ) and water - O - H O(water ) hydrogen bonds with charge - assisted iminium - N (+)- H O(water ) hydrogen bonds providing the connections between the layers to generate the three - dimensional packing . - pubmed
In ( II ) , the chloride anion and water mol - ecules are resolved into two proximate sites with the major component being present with a site occupancy factor of 0.9327 ( 18 ) . - pubmed
gondii infection ( TOX - IgG , TOX - IgM ) was performed , along with individual spermiograms and determinations of follicle - stimulating hormone ( FSH ) and testosterone serum levels . - pubmed
gondii infection on sperm characteristics ( ejaculate quantity , sperm count , motility , morphology ) and serum levels of FSH or testosterone were found . - pubmed
Despite their phylogenetic divergence , the geographically separate Calescamantes populations were highly similar in their predicted metabolic capabilities and core gene content , respiring O2 , or oxidized nitrogen species for energy conservation in distant but chemically similar hot springs . - pubmed
SIRT6 , a member of the mammalian sirtuins family , functions as a mono - ADP - ribosyl transferase and NAD(+)-dependent deacylase of both acetyl groups and long - chain fatty acyl groups . - pubmed
Glu106 targeted inhibitors of ORAI1 as potential Ca2 + release - activated Ca2 + ( CRAC ) channel blockers - molecular modeling and docking studies . - pubmed
Calcium release - activated calcium modulator 1(ORAI1 ) is an integral component of the calcium release - activated calcium channel ( CRAC ) channel complex and plays a central role in regulating Ca2 + concentrations in T - lymphocytes . - pubmed
In this study , we have used a high - throughput screening approach to inhibit the binding of Ca2 + toward ORAI1 and the interactions are verified through induced fit docking . - pubmed
The results hint that these compounds act by possibly binding with , and thereby blocking Ca2 + -binding with ORAI1 ( E106 ) . - pubmed
We have thus identified a novel class of highly stable , potential lead compounds that directly bind with the selectivity filter region E106 and block Ca2 + binding on ORAI1 . - pubmed
Two quantities , the NM - related index ( RNM ) and the binding potential of the radiotracer [ 18F ] FE - PE2I to DAT ( BPND ) in SN , were measured for each subject using MRI and PET , respectively . - pubmed
Development and validation of a sensitive and selective LC - MS / MS method for determination of tacrolimus in oral fluids . - pubmed
In this study , we report the development and validation of a rapid , sensitive and selective liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) method for quantification of tacrolimus in OF . - pubmed
Chromatographic separation were achieved on an Acquity UPLC BEH C18 column by a gradient elution using 2mM ammonium acetate/0.1 % ( v / v ) formic acid in water ( mobile phase A ) and in methanol ( mobile phase B ) with a 2.2min chromatographic run time . - pubmed
Eighteen - month - old date palm plants were subjected to severe salt ( 48 g / L NaCl ) and drought ( 82.5 g / L PEG or no irrigation ) conditions for one month . - pubmed
Transketolase was significantly changed only in drought stress with PEG . - pubmed
Palladium - catalyzed heteroannulation of [ 60 ] fullerene with phenols has been developed as a practical and efficient protocol for the one - pot synthesis of various [ 60 ] fullerene - fused benzofurans . - pubmed
If the incident energy spectrum is known , the dose measured in the PSD 's polystyrene sensitive volume can be converted to score dose in any other media such as air , water or soft tissues using the large cavity theory ( LCT ) . - pubmed
Molecular docking studies revealed that all compounds except PGG showed binding energy values ranging from -10.30 to -8.00kcal / mol while the binding energy of galantamine , a known AChE inhibitor , was -9.53kcal / mol ; they inhibited the AChE by binding into the ligand pocket with the similar binding pattern to that of galantamine by interacting with Glu199 of AChE. - pubmed
The interaction between AChE and PGG was further evaluated with the ITC method and the results indicated that the PGG - AChE interaction was relevant to AChE concentration . - pubmed
Rate of oxygen consumption decreased by 10 % from IS to SS ( IS : 37.9 + - 5.68 ml kg-1 min-1 , SS : 34.1 + - 5.07 ml kg-1 min-1 , P & lt ; 0.0001 , ES 3.05 ) . - pubmed
The neurobiological mechanisms underlying antidepressant effects of CHM , including enhancement of monoamine , dehyperactivation of hypothalamic - pituitary - adrenal axis , neuroimmune response , and neurotrophic factors are discussed . - pubmed
Extracellular ATP is perceived by a plant purinoceptor , P2 receptor kinase 1 ( P2K1 ) , inducing downstream signaling for defense responses . - pubmed
Therefore , enhancing the sensitivity and effect of radiotherapy with propranolol , a beta - adrenoceptor antagonist , could reduce tumor growth . - pubmed
Decreased expression of NF - kappaB was also observed in treatment groups where both propranolol and radiation were used , leading to the reduction of COX-2 , EGFR , and VEGF expression compared to that in the other groups . - pubmed
Conclusion : The present study indicated that propranolol potentiates the antitumor effects of radiotherapy in gastric cancer by inhibiting NF - kappaB expression and its downstream genes : VEGF , EGFR , and COX-2 . - pubmed
Anti - mitochondrial drugs ( oligomycin , casiopeina II - gly , and methoxy - TEA ) and celecoxib , at doses used to block OxPhos , significantly decreased TN cancer cell proliferation ( IC50 = 2 - 20 muM ) , migration capacity ( 10 - 90 % ) , and invasiveness ( 25 - 65 % ) . - pubmed
Methyl salicylate elicited emissions of benzenoids ( BZ ) , monoterpenes ( MT ) and fatty acid derived compounds ( LOX products ) . - pubmed
Although the share of LOX products was similar across MeSA treatments , some LOX products responded differently to MeSA concentration , ultimately resulting in unique VOC blends . - pubmed
It is predicted to be localized in the plasma membrane and may function in transmembrane ion transport and/or as a cell surface receptor . - pubmed
Islet - Derived eATP Fuels Autoreactive CD8 + T Cells and Facilitates the Onset of Type 1 Diabetes . - pubmed
Extracellular ATP ( eATP ) activates T cells by engaging the P2X7R receptor . - pubmed
eATP / P2X7R signaling facilitates the onset of autoimmune T1D by fueling autoreactive CD8 + cells and therefore represents a novel targeted therapeutic for the disorder . - pubmed
To interrupt neuronal transmission in the spinal defecation center , antagonists of norepinephrine , dopamine , and/or serotonin receptors were injected intrathecally at the L6 - S1 spinal level , where the spinal defecation center is located . - pubmed
Intraluminal administration of capsaicin , acting on the transient receptor potential vanilloid 1 channel , caused transient propulsive contractions . - pubmed
Capsaicin - induced contractions were blocked by preinjection of D2 - like dopamine receptor and 5 - hydroxytryptamine subtype 2 and 3 receptor antagonists into the spinal defecation center . - pubmed
sAA activity did not correlate with sAA concentration or salivary cortisol , and a low correlation was observed between sAA concentration and salivary cortisol ( r = 0.48 , p = 0.003 ) . - pubmed
Methods : Five high - touch surfaces were cleaned and disinfected either according to the SOP ( alcohol - based cleaning and chlorine - based disinfection ) or using quaternary ammonium compounds - based disposable wipes ( MOP ) . - pubmed
In this study , we examined the effectiveness of a hallmark DOR agonist , SNC80 , in disease models reflecting diverse headache disorders including : chronic migraine , post - traumatic headache ( PTH ) , medication overuse headache by triptans ( MOH ) , and opioid - induced hyperalgesia ( OIH ) . - pubmed
PTH was modeled by combining the closed head weight drop model with the nitroglycerin model of chronic migraine . - pubmed
Engineered Ssp DnaX inteins for protein splicing with flanking proline residues . - pubmed
We found that engineered Ssp DnaX mini - intein and split inteins could carry out protein splicing with proline at the +2 positions or at both -1 and +2 positions . - pubmed
coli cells , the mini - intein , S1 split intein , and S11 split intein spliced efficiently , whereas the S0 split intein did not splice with proline at both -1 and +2 positions . - pubmed
The S1 and S11 split inteins also trans - spliced efficiently in vitro with proline at the +2 positions or at both -1 and +2 positions , but the S0 split intein trans - spliced inefficiently with proline at the +2 position and did not trans - splice with proline at both -1 and +2 positions . - pubmed
Nine RCTs that compared hybrid CR with usual care showed that hybrid CR had greater improvements in peak oxygen uptake ( 9.72 mL / kg per minute ; 95 % CI , 5.12 - 14.33 ) but not in hr - QOL ( 2 studies ; standardized mean difference : 0.67 ; 95 % CI , -0.20 to 1.54 ) . - pubmed
Specially , the down - regulation of nicotinamide ( NAM ) upon SK treatment indicated the suppression of the deacetylation of the histone H3 on lysine 56 residue ( H3K56 ) . - pubmed
CONCLUSIONS : UGT1A1 polymorphisms were associated with improved outcomes , despite pazopanib interruption and dose reductions . - pubmed
In era of antibiotic resistance , antibacterial silver nanoparticles are considered as potential alternative therapeutic agent to combat drug resistant pathogens . - pubmed
Evolving a Unique Red - emitted Fluorophore with Optically Tunable Hydroxy Group for Imaging Nitroreductase in Cell , Tissue and Vivo . - pubmed
The cell debris solutions and filter liquor from inducible and non - inducible samples were cultured with 3 - PBA and its intermediate metabolites at same condition , and the location and induction of enzymes were analyzed by the degradation . - pubmed
This study provided a promising method for studying the co - metabolic enzymes of 3 - PBA degradation by fungi . - pubmed
Polymorphic alpha - Synuclein Strains Modified by Dopamine and Docosahexaenoic Acid Interact Differentially with Tau Protein . - pubmed
Our institution began using front - loaded lorazepam in August 2017 secondary to a nation - wide shortage of intravenous ( IV ) diazepam . - pubmed
CONCLUSION : Front - loaded lorazepam was similar to front - loaded diazepam in controlling AWS symptoms . - pubmed
PARP1 catalytic variants reveal branching and chain length - specific functions of poly ( ADP - ribose ) in cellular physiology and stress response . - pubmed
Poly ( ADP - ribose ) ( PAR ) molecules exhibit a remarkable heterogeneity in chain lengths and branching frequencies , but the biological significance of this is basically unknown . - pubmed
Adjuvants are mixed with the immunizing antigen for the first two immunizations only , and Complete Freund 's adjuvant is only used with the first immunization ; subsequent immunizations are performed in phosphate - buffered saline ( PBS ) or normal saline , with or without Incomplete Freund 's adjuvant . - pubmed
The impacts of TIG on the level of inflammatory cytokines ( IL-1beta , TNF - alpha , IL-6 , IFN - gamma , IL-17 and IL-10 ) , and the regulation of OPG / RANKL / NF - kappaB pathways were determined by the ELISA and western blot , respectively . - pubmed
Moreover , TIG markedly inhibited the expression of p - IKK - alpha , p - IkappaB and p - p65 , and decreased the ratio of OPG / RANKL in the synovial tissues . - pubmed
CONCLUSION : TIG possessed significant anti - RA activities on adjuvant - induced arthritis , which might be ascribed to the regulation of inflammatory cytokines IL-1beta , TNF - alpha , IL-6 , IFN - gamma IL-17 and IL-10 , as well as inhibition of OPG / RANKL / NF - kappaB signaling pathways . - pubmed
The prepared CuO / ZnO - DSDSHNM was used over the glassy carbon electrode ( GCE ) as the active material for glucose detection and then coated by Nafion to provide the proposed Nafion / CuO / ZnO - DSDSHNM / GCE . - pubmed
The achieved high - performance glucose sensing based on Nafion / CuO / ZnO - DSDSHNM / GCE shows that both the material synthesis and the sensor fabrication methods have been promising and they can be used in future researches . - pubmed
Asymmetric binding of the inhibitor di ( adenosine-5 ' ) pentaphosphate ( Ap5A ) to adenylate kinase . - pubmed
The effect of binding diadenosine pentaphosphate ( Ap ( 5 ) A ) to adenylate kinase ( ATP : AMP phosphotransferase ; EC 2.7.4.3 ) has been investigated by ( 31 ) P nuclear magnetic resonance . - pubmed
The symmetric molecule , Ap(5)A , is a potent inhibitor of the adenylate kinase reaction , 2 ADP right arrow over left arrow ATP + AMP . - pubmed
The spectrum of Ap ( 5 ) A bound to porcine adenylate kinase shows five groups of signals centered at 10.9 , 11.9 , 20.5 , 22.7 , and 24.0 ppm ; the resonances at 11.1 ppm ( 1 - P and 5 - P ) and at 22.8 ppm ( 2 - P and 4 - P ) are now clearly split , indicating asymmetric binding of Ap ( 5 ) A to the enzyme . - pubmed
The asymmetry is strikingly enhanced in enzyme - bound MgAp ( 5 ) A , which has resonances at 10.5 , 12.5 , 18.6 , 22.7 , and 25.6 ppm . - pubmed
By the addition of Mn ( 2 + ) to the enzyme . - pubmed
MgAp ( 5 ) A complex , the observed signals in increasing order of shifts were tentatively assigned to 1 - P , 5 - P , 4 - P , 3 - P , and 2 - P , where the 3- , 4- , and 5 - P positions correspond to the ATP - binding site on the enzyme . - pubmed
The asymmetry introduced in the phosphate chain of enzyme . - pubmed
MgAp ( 5 ) A is indicated by the ( 31 ) P chemical shift of 7 ppm between 2- and 4 - P , which is one of the largest thus far observed for phosphate substrates bound noncovalently to enzymes . - pubmed
If this has been ruled out and if oestrogens are elevated with low plasma gonadotrophins , a feminizing ovarian or adrenal tumour should be considered . - pubmed
cholerae 0395 containing a TnphoA insertion in a gene designated viuA , lacks this 74 - kDa outer membrane protein and is unable to bind or utilize exogenous ferric vibriobactin . - pubmed
Introduction of a plasmid containing the complete viuA coding sequence and 513 bp of upstream DNA into MBG14 restored ferric vibriobactin utilization to the mutant . - pubmed
Construction of viuA::TnphoA gene fusions allowed study of the regulation of viuA expression by iron . - pubmed
cholerae grown in high- and low - iron media revealed a monocistronic viuA message that was negatively regulated by iron at the transcriptional level . - pubmed
cholerae by fur ; additional regulatory factors , superimposed upon the fur system , may provide more precise control of individual iron - regulated genes . - pubmed
Because pentoxifylline ( PTXF ) inhibits TNF production , we hypothesized that PTXF would ameliorate IL-2 toxicity without compromising antitumor efficacy . - pubmed
Upon completion of therapy , we found that PTXF suppressed many of the IL-2 - induced effects including TNF production , lymphocytic infiltration of multiple organs , multiple organ edema , hepatic dysfunction , leukopenia , and thrombocytopenia . - pubmed
PTXF preserved antitumor efficacy while reducing the morbidity and mortality caused by IL-2 treatment . - pubmed
These data strongly support the use of PTXF in extending the therapeutic index of IL-2 in the treatment of cancer . - pubmed
The dopamine ( D2 ) blocker , haloperidol , did not block AMPH actions . - pubmed
One group had large HDL particle sizes and high HDL - cholesterol concentrations . - pubmed
Another group had smaller HDL particles and lower HDL - cholesterol concentrations , and HDL sizes and HDL - cholesterol concentrations were significantly correlated . - pubmed
In the RI strains , HDL sizes and HDL - cholesterol cholesterol concentrations clearly segregated with one or another of the progenitor strains , and RI strain distributions showed a strong linkage to the apolipoprotein ( apo ) A - II gene ( Apoa-2 ) . - pubmed
In contrast , LDL - cholesterol concentrations and particle sizes on FPLC did not show dichotomous distributions among the 10 inbred strains . - pubmed
In RI strains , the configuration of the LDL FPLC profiles and LDL - cholesterol concentrations did resemble one or another of the progenitors in the majority of cases , but LDLs of several RI strains resembled neither progenitor strain in profile configuration , and LDL - cholesterol concentrations were both greater and smaller than those of progenitor strains . - pubmed
However , LDL particle diameters ( as judged by peaks of LDL - cholesterol profiles ) did segregate with progenitors in 29/33 ( 88 % ) of RI strains suggesting that a major gene may affect LDL size . - pubmed
Dexamethasone inhibits the induction of monocyte chemotactic - activating factor production by IL-1 or tumor necrosis factor . - pubmed
We examined the turn - over of synthesized MCAF mRNA that showed DXS decreased the stability of MCAF mRNA . - pubmed
In addition , a nuclear run - off analysis revealed that DXS also inhibited the transcription of IL-1- or TNF - activated MCAF genes . - pubmed
Therefore , both the destabilization of MCAF mRNA and the inhibition of transcription of the gene contribute to the decrease in the MCAF mRNA steady state level by DXS . - pubmed
One porter developed high altitude pulmonary edema , Grade IV , at an altitude of 4,200 m , and was successfully treated with oxygen , nifedipine and descent . - pubmed
A second precolumn packed with C18 - bonded silica was used for the reconcentration of desorbed clenbuterol prior to the analytical separation . - pubmed
Urine , after 2 - fold dilution with buffer ( pH 7.4 ) , was loaded directly onto the immuno precolumn , where clenbuterol was trapped by the immobilized antibodies . - pubmed
The sustained negative potential and the negative OFF response were not the M - wave ON and OFF responses of proximal retina , despite an overall resemblance in form and time course : 1 ) the M - wave was spatially tuned , whereas the ERG components were not ; 2 ) tetrodotoxin ( TTX ) ( 3.8 - microM vitreal concentration ) did not alter the M - wave , but it reduced the ERG OFF response ; 3 ) picrotoxin ( 0.14 mM , after TTX ) enhanced the M - wave but did not affect the negative ERG ; and 4 ) 2 - amino-4 - phosphonobutyric acid ( APB ; 0.95 mM ) removed the M - wave ON response , and aspartate ( 43 mM ) removed the M - wave OFF response , in addition , while the sustained negative potential persisted . - pubmed
The number of amino acids between the two conserved cysteine residues is intermediate between those of the Ig V and C domains . - pubmed
Likewise , a clear - cut synergism was observed when a low dose of the dopamine receptor agonist apomorphine ( 0.1 mg / kg ) , which did not per se affect motor activity , was combined with MK-801 ( 1.5 mg / kg ) ; however , the synergism between apomorphine and MK-801 was less dramatic than that observed between MK-801 and clonidine . - pubmed
Removing extracellular Na + prevented the development of the negative slope in this voltage region , suggesting that Na + can block IK1 channels in a time- and voltage - dependent manner . - pubmed
The time and voltage dependence of Cs +-induced block of IK1 was also examined . - pubmed
In the absence of Na + , inactivation of IK1 could also be induced by both Cs + and Li + . - pubmed
The degradation of 3H - TP60 in the absence of RNA synthesis was inhibited by iodoacetamide , a cysteine protease inhibitor , resulting in the accumulation of 3H - TP60 in the nucleus reaching about a threefold concentration in the cytoplasm . - pubmed
The NADPH - nitrate reductase enzymes formed in the above three complementing mixtures are similar to the wild - type enzyme in sucrose density gradient profiles , molecular weight , substrate affinity , sensitivity to inhibitors and temperature , but show different ratios of associated enzyme activities . - pubmed
The data suggest that nitrate reductase consists of at least two protein subunits : a nitrate - inductible subunit as reflected by inductible NADPH - cytochrome c reductase , and a constitutive protein which is activated ( as indicated by the appearance of flavine adenine dinucleotide , reduced form ( FADH ( 2 ))- and reduced methyl viologen - nitrate reductase activities ) when it combines with the inductible subunit . - pubmed
3 - bromoacetonyltrimethylammonium bromide , a choline acetylase inhibitor . - pubmed
[ Lack of activity of diphenhydramine in the secretion of prolactin and insulin induced by arginine ] . - pubmed
Membranes prepared throughout in N - ethylmaleimide , which yield upon detergent solubilization a predominant 13 - S dimeric species and which are not depleted of the nonreceptor v - peptide ( Mr 43 000 ) by alkaline treatment , are relatively more ' rigid ' than normal membranes and do not show the anisotropy components characteristic of the monomeric receptor . - pubmed
The influence of the v - peptide on the structural stability of the receptor oligomeric forms and the thiol - dependent interconversions are thus reflected in the time - dependent spectroscopic measurements . - pubmed
[ Pro4 , Trp7,9 ] SP-(4 - 11 ) and a newly developed compound , [ Pro4 , Trp7,9,10 ] SP-(4 - 11 ) were shown to be potent antagonists of substance P ( SP ) . - pubmed
Water versus acetone - HCl extraction of digitalis - like factor from guinea - pig heart . - pubmed
The enzyme was maximally activated , approximately 2 - fold , in the presence of 0.1 - 0.4 % ( w / v ) digitonin . - pubmed
Induction of the digitonin activated clofibric acid glucuronyltransferase was observed following phenobarbitone treatment in vivo ( 2.2 - fold ) , and to a lesser extent , following beta - naphthoflavone treatment ( 1.3 - fold ) . - pubmed
0.08 nmoles glucuronide formed / min / mg microsomal protein ) . - pubmed
The data indicated that clofibric acid glucuronyltransferase belongs to the neonatal cluster of enzymes and clofibric acid is a group 2 substrate . - pubmed
An unusual pattern of peptide - bound lysine metabolism in collagen from an infant with lethal perinatal osteogenesis imperfecta . - pubmed
It was subsequently shown that the apparent deficiency in lysyl residues was due , at least in part , to the presence of unusually high levels of allysine , a cross - link precursor formed from peptide - bound lysine under the catalytic action of lysyl oxidase . - pubmed
The inhibitory effect of MTX - poly ( Lys ) on dihydrofolate reductase in vitro is increased nearly 100 - fold in the presence of excess heparin but remains less than that of free MTX . - pubmed
These observations raise the possibility that at least part of the MTX - poly ( Lys )- heparin reaches cellular dihydrofolate reductase in the form of macromolecular complexes that escape from entrapment in endocytotic structures . - pubmed
Activities of glycolytic enzymes in the pectoralis muscle showed similar patterns of development as those found for the aerobic enzymes , except for hexokinase , which was low at all ages , indicating an adaptation for catabolism of both intracellular glycogen and plasma fatty acids in preference to plasma glucose . - pubmed
Activities of anaerobic glycolytic enzymes in the ventricles were low , but values for hexokinase , which is involved in the phosphorylation of plasma glucose , developed rapidly . - pubmed
Values for lactate dehydrogenase were also high , reflecting the capacity of the heart to catabolize plasma lactate . - pubmed
The average maximum oxygen uptake for premigratory geese during flight , as indicated by values for OGD , is calculated to be 484 ml O2 / min ( or 208 ml O2.min-1.kg-1 ) . - pubmed
ADP - ribosylation is a reversible post - translational modification of proteins involving the addition of the ADP - ribose moiety of NAD to an acceptor protein or amino acid . - pubmed
The reverse reaction , catalyzed by ADP - ribosylarginine hydrolase , removes ADP - ribose , regenerating free arginine . - pubmed
IGF - I and insulin both stimulated 3 - O - methylglucose uptake and GLUT-4 translocation in a dose - dependent manner with a maximal effect six- to sevenfold above basal . - pubmed
No additive effects of IGF - I and insulin on maximal 3 - O - methylglucose uptake were found . - pubmed
In contrast , IGF - I bound to the partially purified IGF - I receptor with an apparent affinity ( ED50 = 3.7 nM ) that was similar to the concentrations of IGF - I which caused half - maximal activation of 3 - O - methylglucose uptake ( ED50 = 2.4 nM ) and GLUT-4 translocation ( ED50 = 2.5 nM ) . - pubmed
Our findings suggest that IGF - I exerts its insulin - like effects on glucose uptake primarily through its own specific receptor and that the molecular events underlying IGF - I and insulin actions on glucose uptake in skeletal muscle are similar , namely caused by a translocation of the GLUT-4 transporter from an intracellular pool to the cell surface . - pubmed
This was done by the determination of progesterone production , aromatase activity and cAMP accumulation after a 48 h incubation with PMSG , IGF - I and PMSG plus IGF - I in cells treated with either CT or PT . - pubmed
Both CT and PT treatment stimulated PMSG - induced progesterone production in granulosa cells after 48 h of culture with PMSG . - pubmed
CT treatment also stimulated aromatase activity in cells treated with PMSG and increased cAMP secretion under basal conditions ( untreated cells ) and in PMSG treated cells . - pubmed
Both CT and PT increased the stimulation by IGF - I of PMSG - induced progesterone production after 48 h of culture with PMSG plus IGF - I. - pubmed
Furthermore , CT augmented the enhancement by IGF - I of PMSG - induced aromatase activity and cAMP accumulation . - pubmed
In the absence of PMSG , CT did not increase steroidogenesis either alone or in the presence of IGF - I within the time frame studied even though CT was able to stimulate cAMP accumulation in untreated and IGF - I treated cells . - pubmed
After concentration of amoxicillin on a C8 cartridge , the cartridge was eluted on - line to a reversed - phase column packed with 5 microns Chromspher C18 . - pubmed
The organophosphonate recruited chemical potential from the immune response that resembled the way these compounds recruit the catalytic power of the serine hydrolases . - pubmed
During this recruitment , a large proportion of the isolated antibodies catalyzed the formation and cleavage of phosphonylated intermediates and subsequent ester hydrolysis . - pubmed
Furthermore , MN frequencies obtained from slides stained with May - Grunwald - Giemsa ( MGG ) and with the DNA fluorochrome 4',6 '- diamidino-2 - phenylindole ( DAPI ) were compared to check if the DNA - specific DAPI facilitated a more accurate recording of MN than the unspecific MGG . - pubmed
Alachlor ( 7.5 - 20 micrograms / ml ) and MMC ( 0.6 microM ) acted as pure clastogens without aneugenic activity , inducing exclusively KC- and CM - negative MN . - pubmed
VBL produced primarily KC- and CM - positive MN , in accordance with its known mechanism of action . - pubmed
A comparison between CM and KC data in the VBL treatment suggested that some 7 % of KC - containing MN may not be detected by the probe . - pubmed
The frequencies of MN were generally higher in slides stained with DAPI than in those stained with MGG , especially in controls and clastogen - treated cultures. - pubmed
We also studied the induction of 7 - ethoxyresorufin - O - deethylase ( EROD ) in these PCB - treated cells . - pubmed
Microsequencing of the 50 - kDa band yielded an amino - terminal sequence of 38 amino acids identical to that deducted from the CYP2D16 cDNA . - pubmed
Sodium dodecyl sulfate - polyacrylamide gel electrophoresis and Western blot analyses demonstrated greater expression of CYP2D16 in microsomes from the inner zone ( zona reticularis ) of the adrenal cortex than from the outer zones , coinciding with the major site of adrenal xenobiotic metabolism . - pubmed
Actin disruption inhibits bombesin stimulation of focal adhesion kinase ( pp125FAK ) in prostate carcinoma . - pubmed
UNLABELLED : Jasplakinolide is a member of a new class of antitumor agents targeting the actin cytoskeleton with activity against prostate cancer . - pubmed
We hypothesized that the neuropeptide bombesin would activate FAR in prostate carcinoma , and that disruption of the actin network would block FAK activation and inhibit cell growth . - pubmed
FAK phosphorylation was measured in cells stimulated with 0.01 - 10 nM bombesin ; separate cells were pretreated 6 hr with 50 - 500 nM Jas or CyE. - pubmed
Cell lysates and anti - FAK immunoprecipitates were subjected to SDS - PAGE , Western blotting , and detection with anti - actin or anti - phosphotyrosine . - pubmed
Bombesin rapidly stimulated tyrosine phosphorylation of FAK in a dose dependent manner . - pubmed
FAK phosphorylation was inhibited to near - basal levels ( 50 % of bombesin stimulated ) by 500 nM Jas ( 63 % ) and 500 nM CyE ( 61 % ) . - pubmed
CONCLUSIONS : Bombesin stimulated FAK in prostate carcinoma cells . - pubmed
Substrate nucleotide - determined non - templated addition of adenine by Taq DNA polymerase : implications for PCR - based genotyping and cloning . - pubmed
The Applied Biosystems PRISM fluorescence - based genotyping system as well as the Invitrogen TA Cloning vector system are influenced by the tendency of Taq DNA polymerase to add an adenine nucleotide to the 3 ' end of PCR products after extension . - pubmed
Lysates of C57BL/6J - derived BMMC contained a Ca2 + -dependent PLA2 that was absorbed to a column conjugated with anti - type IIA sPLA2 antibody and had a similar molecular mass of 14 kDa , as assessed by immunoblotting . - pubmed
The soluble mineralocorticoid receptor bound to an agonist acts as a transcription factor for several genes relevant to ion transport by kidney and colon epithelial cells and is a major regulator of electrolyte and fluid homeostasis . - pubmed
The isolated mineralocorticoid receptor binds several adrenal steroids , including aldosterone and the major glucocorticoids , with equal affinity . - pubmed
Evidence is presented for the de novo synthesis of adrenal corticosteroids in the brain which may , by paracrine regulation of central control mechanisms , be relevant for certain clinical and experimental forms of hypertension characterized by low circulating levels of mineralocorticoids which respond to mineralocorticoid receptor antagonists . - pubmed
During the course of our analysis of myo - inositol monophosphatase ( IMPase ) , a key enzyme of brain inositol signaling , we found it also hydrolyzes galactose 1 - phosphate ( Gal 1 - P ) , an intermediate of galactose metabolism . - pubmed
The ability of IMPase to use both inositol 1 - phosphates and galactose 1 - phosphate equally as substrates is of considerable importance in determining lithium 's mechanism of action . - pubmed
Our current results suggest that during lithium therapy , both galactose and inositol metabolic pathways can be simultaneously modulated through lithium inhibition of IMPase . - pubmed
Enzyme studies with Mg2 + ions as activators and with Li + , Ca2 + , Mn2 + , Ba2 + ions as inhibitors demonstrate that IMPase is a single enzyme possessing the ability to hydrolyze both inositol monophosphates and Gal-1 - P with equal efficiency . - pubmed
Our results indicate that lithium inhibition of IMPases at clinically relevant concentrations , may modulate both inositol and galactose metabolism , and identifies yet another carbohydrate pathway utilizing IMPase . - pubmed
On postoperative day 3 , if both a pneumothorax and AL were present , the CT was placed to 10 cm H2O of suction . - pubmed
If a pneumothorax was present without an AL , the CT was returned to 20 cm H2O of suction . - pubmed
Oxidative modification of glutamine synthetase by 2,2 '- azobis ( 2 - amidinopropane ) dihydrochloride . - pubmed
To this end , we exposed glutamine synthetase ( GS ) and the peptide melittin to solutions containing 2,2 '- azobis ( 2 - amidinopropane ) dihydrochloride ( AAPH ) , which is known to decompose in aqueous , aerobic solutions to yield alkyl radicals and alkylperoxides . - pubmed
Under our conditions , pH 7.4 , 37 degrees C , the AAPH - dependent formation of alkylhydroperoxide increased linearly with time and led to 40 % inactivation of GS in 1 h and to complete inactivation in 4 h. Complete inactivation was associated with the loss of 2 of 16 histidine residues , 6 of 17 tyrosine residues , 5 of 16 methionine residues , and all of the tryptophan residues ( 2 residues ) per subunit . - pubmed
Exposure of GS to AAPH led also to the generation of protein carbonyl derivatives ( 0.34 mol / mol subunit ) and to formation of a significant amount ( 0.038 mol / mol subunits ) of quinoprotein derivatives . - pubmed
To investigate the mechanism of tryptophan modification , the 26 - amino - acid peptide , melittin , which contains one tryptophan but no histidine , tyrosine , or methionine residues , was treated with AAPH . - pubmed
Stimulation with lipopolysaccharide and IL-1beta resulted in the full induction of IL-6 expression only if the cells were coincubated with cAMP agonists ; this effect was attenuated by protein kinase A inhibitors . - pubmed
Inhibition of NO - synthase by the intravenous administration of L - NNA reduced significantly the amplitude of neurotensin hyperaemia . - pubmed
Pretreatment with L - Arginine , the substrate of NO - synthase reversed the hemodynamic , but not motility effects of neurotensin . - pubmed
Neuronal nicotinic receptors in dementia with Lewy bodies and schizophrenia : alpha - bungarotoxin and nicotine binding in the thalamus . - pubmed
AIM : To study antianginal effectiveness of trimetazidine ( TMS ) in monotherapy and combined antianginal therapy ; to design approaches to prediction of its effect . - pubmed
The proteins , which were named grainin 1 and 2 , show a considerable structural similarity to calcium - binding proteins , particularly within EF - hand motifs . - pubmed
Each grainin possesses three of these putative calcium - binding sites . - pubmed
Calcium - binding activity of grainins was demonstrated by two independent methods . - pubmed
Dependent variables for the study were total cholesterol ( TC ) , triglycerides ( TG ) , high - density lipoprotein cholestrol ( HDL - C ) , low - density lipoprotein cholesterol ( LDL - C ) , and systolic ( SBP ) and diastolic ( DBP ) blood pressure . - pubmed
Identification of Cys140 in helix 4 as an exofacial cysteine residue within the substrate - translocation channel of rat equilibrative nitrobenzylthioinosine ( NBMPR )- insensitive nucleoside transporter rENT2 . - pubmed
PCMBS caused reversible inhibition of uridine influx by rENT2 , but had no effect on hENT1 , hENT2 or rENT1 . - pubmed
This difference correlated with the presence in rENT2 of a unique Cys residue ( Cys(140 ) ) in the outer half of TM4 that was absent from the other ENTs . - pubmed
Mutation of Cys(140 ) to Ser produced a functional protein ( rENT2 / C140S ) that was insensitive to inhibition by PCMBS , identifying Cys(140 ) as the exofacial Cys residue in rENT2 responsible for PCMBS inhibition . - pubmed
Uridine protected wild - type rENT2 against PCMBS inhibition , suggesting that Cys(140 ) in TM4 lies within or is closely adjacent to the substrate - translocation channel of the transporter . - pubmed
Attenuation of hypothermia - induced platelet activation and platelet adhesion to artificial surfaces in vitro by modification of hemoglobin to carry S - nitric oxide and polyethylene glycol . - pubmed
The effects of human hemoglobin ( Hb ) covalently modified to carry S - nitric oxide ( NO ) functional groups ( SNO - Hb ) , polyethylene glycol ( PEG - Hb ) , and SNO - PEG - Hb on platelet activation were studied . - pubmed
Platelet adhesion and aggregation were measured in a parallel glass plate chamber coated with unmodified Hb , SNO - Hb , PEG - Hb , SNO - PEG - Hb , and collagen . - pubmed
There was significantly less platelet binding of antibodies to GPIIb - IIIa and P - selectin with SNO - Hb , PEG - Hb , and SNO - PEG - Hb compared with unmodified Hb . - pubmed
There was significantly less platelet attachment , adhesion , and aggregation on the SNO - Hb , PEG - Hb and SNO - PEG - Hb coated surfaces compared with unmodified Hb - coated and -uncoated surfaces . - pubmed
SNO - Hb , PEG - Hb , and SNO - PEG - Hb induced less platelet activation at hypothermic temperature , and induced less platelet adhesion and aggregation on thrombogenic surfaces compared with unmodified Hb . - pubmed
The inhibitory effect may be derived from antiadhesive properties of Hb , antiplatelet actions of NO , and molecular barrier action of PEG . - pubmed
Investigation of the levels of cyclins E , D and B by immunoblot analysis showed cyclin B level was unaffected , whereas cyclin D and E levels declined with curcumin in both cell lines . - pubmed
Investigation of cyclin - dependent kinases , Cdk2 and Cdc2 , showed activity of Cdc2 , but not Cdk2 , increased markedly in response to curcumin . - pubmed
In both cell lines , immunoblot analysis indicated that curcumin caused induction of apoptosis as evidenced by cleavage of PARP , caspase-3 , and reduction in Bcl - XL levels . - pubmed
Curcumin also stimulated the activity of caspase-8 , which initiates Fas signalling pathway of apoptosis . - pubmed
O-15 water CBF images can predict the effect of Nicholin by assessing transient increases in cerebral blood flow ( CBF ) , thereby facilitating improvement in higher brain functions such as orientation . - pubmed
Brain SPECT with I-123 IMP can also identify " misery " perfused areas resulting from reduced CBF and decreased vasoreactivity in the chronic phase . - pubmed
The inclusion of hexafluoroisopropanol in the cleavage solvent mixture did not improve yields for either gA or WALP peptides . - pubmed
For example , higher estrogen levels , elevated insulin levels , a greater concentration of growth factors in adipose tissue , hypertension , cholesterol metabolism abnormalities , and immune malfunction are just some of the potential mechanisms that may increase kidney cancer risk . - pubmed
There was also an association between BNP and male sex ( p = 0.008 ) , higher plasmatic creatinine ( p = 0.01 , r = 0.25 ) , Iarger ventricular diameters ( p = 0.0001 ) and higher pulmonary systolic pressure ( p = 0.001 , r = 0.44 ) . - pubmed
The FP region is composed of hydrophobic , glycine - rich residues that are essential for the initial penetration of the target cell membrane . - pubmed
Primary management of post - radiation bone lesions include conservative modalities such as saline irrigations , antibiotics during infectious episodes , topically applied antiseptics , gentle sequestrectomy and removal of visibly loosened bone elements as well as treatment with hyperbaric oxygen ( HBO ) . - pubmed
Human serum and glucocorticoid - inducible kinase - like kinase ( SGKL ) phosphorylates glycogen syntheses kinase 3 beta ( GSK-3beta ) at serine-9 through direct interaction . - pubmed
Furthermore , the kinase activity of wild - type SGKL was required for the in vitro phosphorylation of a GSK-3 crosstide fusion protein at serine-21/9 as demonstrated with a Phospho - GSK-3alpha / beta ( Ser21 / 9 ) specific antibody . - pubmed
The present results provide strong evidences that SGKL could utilize GSK-3beta as a direct downstream target by phosphorylating GSK-3beta at serine-9 . - pubmed
Pre - administration of glucagon had no effect on serum lactate and pyruvate levels after ischemic forearm exercise test while serum glucose elevated . - pubmed
The glucose clamp test confirmed insulin resistance . - pubmed
In these mutants , several independent insertions mapped in the dltA gene ( involved in D - alanine transfer in lipoteichoic acids ) and resulted in a NucT secretion defect . - pubmed
Metabotropic glutamate receptor type 4 is involved in autoinhibitory cascade for glucagon secretion by alpha - cells of islet of Langerhans . - pubmed
The L - glutamate triggers secretion of gamma - aminobutyric acid ( GABA ) from beta - cells , which in turn inhibits glucagon secretion from alpha - cells through the GABAA receptor . - pubmed
In the present study , we tested the working hypothesis that L - glutamate functions as an autocrine / paracrine modulator and inhibits glucagon secretion through a glutamate receptor(s ) on alpha - cells . - pubmed
The addition of L - glutamate at 1 mmol / l ; ( R , S )- phosphonophenylglycine ( PPG ) and ( S )- 3,4 - dicarboxyphenylglycine ( DCPG ) , specific agonists for class III metabotropic glutamate receptor ( mGluR ) , at 100 micromol / l ; and ( 1S,3R,4S )- 1 - aminocyclopentane-1,3,4 - tricarboxylic acid ( ACPT - I ) at 50 micromol / l inhibited the low - glucose - evoked glucagon secretion by 87 , 81 , 73 , and 87 % , respectively . - pubmed
This inhibition was dose dependent and was blocked by ( R , S )- cyclopropyl-4 - phosphonophenylglycine ( CPPG ) , a specific antagonist of class III mGluR. - pubmed
Agonists of other glutamate receptors , including kainate and quisqualate , had little effectiveness . - pubmed
Pertussis toxin , which uncouples adenylate cyclase and inhibitory G - protein , caused the recovery of both the cAMP content and secretion of glucagon . - pubmed
These results indicate that alpha- and F cells express functional mGluR4 , and its stimulation inhibits secretion of glucagon through an inhibitory cAMP cascade . - pubmed
Testosterone ( 1 microM ) treatment induced a maximum increase in beta1 - adrenergic receptor and L - type calcium channel mRNA levels following an eight hour exposure . - pubmed
Six hours testosterone treatment stimulated a 300 - fold increase in androgen receptor message abundance , and Na / Ca exchanger mRNA levels reached a maximum level following twenty - four hour testosterone treatment . - pubmed
Cluster analysis allowed identification of colinear or antiparallel expression profiles of a small set of genes involved in post - translational regulation , and photosynthetic carbon metabolism . - pubmed
Upon stimulation with lipopolysaccharide / interferon - gamma , macrophages generated a high concentration of nitric oxide . - pubmed
Western blot and reverse transcription polymerase chain reaction analysis showed that melatonin decreased the expression of inducible nitric oxide synthase at either the protein or mRNA level . - pubmed
Further investigation showed that melatonin significantly attenuated the nitration of cytoplasmic IkappaB - alpha , inhibited the degradation of IkappaB - alpha , and blocked the translocation of p65 / RelA into the nuclei . - pubmed
The results suggest that melatonin inhibits the expression of inducible nitric oxide synthase by modulating the nuclear factor kappaB pathway . - pubmed
Protective mechanisms are impermeability of mucosa barrier , presence of intestinal mucus , activity of secretive immunoglobulins , some prostaglandins and interleukins , glutamine , somatostatin , cortisol and short - chain fatty acids . - pubmed
After dosing with the MR extracts , the thickness of the hind limbs , tibia failure load , tibia bone mineral density ( BMD ) , serum osteocalcin levels , tibia calcium ( Ca ) and phosphorus ( P ) contents were monitored with histomorphometrical changes of the tibia . - pubmed
The macrolide roxithromycin impairs NADPH oxidase activation and alters translocation of its cytosolic components to the neutrophil membrane in vitro . - pubmed
Roxithromycin alters the reconstitution of an active enzyme and impairs the translocation to the outer membrane of the cytosolic components p47 - phox and p67 - phox . - pubmed
Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes - chitosan composite . - pubmed
The carbon nanotubes - chitosan ( CNTs - CS ) composite provides a suitable biosensing matrix due to its good conductivity , high stability , and good biocompatibility . - pubmed
The system is in favor of the accessibility of substrate to the active site of laccase , thus the affinity to substrates is improved greatly , such as 2,2 '- azino - bis -( 3 - ethylbenzthiazoline-6 - sulfonic acid ) diammonium salt ( ABTS ) , catechol , and O2 with Km values of 19.86 microM , 9.43 microM , and 3.22 mM , respectively . - pubmed
The fortuitous location of this specific stabilizing interaction in a disulfide - bond - containing loop region of ribonuclease A results in the localized modulation of protein dynamics that , in turn , enhances the susceptibility of the disulfide bond to reduction leading to an alteration in the reductive unfolding behavior of the homologues . - pubmed
RESULTS : ( 1 ) The MVD of the NCTD group was 4.12 + /- 1.4 , significantly lower than those of the 5 - FU group ( 15.8 + /- 5.9 ) and control group ( 17.6 + /- 3.2 ) ( both P & lt ; 0.01 ) , but not significantly different from those of the NCTD + 5 - FU group ( 3.8 + /- 1.7 ) , ES group ( 4.5 + /- 2.1 ) , and NCTD + ES group ( 2.9 + /- 1.5 ) ( all P & gt ; 0.05 ) . - pubmed
( 3 ) In comparison with the control group , the mRNA expression of VEGF and of Ang-2 of the tumor cells of the NCTD group were both significantly lower and the mRNA expression of TIMP2 was significantly higher . - pubmed
The PBR binds to high - affinity drug ligands and cholesterol . - pubmed
Many functions are associated directly or indirectly with the PBR , including the regulation of cholesterol transport and the synthesis of steroid hormones , porphyrin transport and heme synthesis , apoptosis , cell proliferation , anion transport , regulation of mitochondrial functions and immunomodulation . - pubmed
By using terminal deoxynucleotidyl transferase - mediaed dUTP nick end labeling ( TUNEL ) , apoptotic cells were detected in 40 specimens of breast cancer . - pubmed
Multifrequency EPR studies on the Mn ( II ) centers of oxalate decarboxylase . - pubmed
A multifrequency cw - EPR approach has now enabled us to show that the two Mn ions can be distinguished on the basis of their differing fine structure parameters and to observe that acetate and formate bind to Mn ( II ) in only one of the two sites . - pubmed
The EPR evidence is consistent with the hypothesis that this Mn - binding site is located in the N - terminal domain , in agreement with predictions based on a recent X - ray structure of the enzyme . - pubmed
BP554 is a 5 - HT1A agonist acting primarily on the membrane of efferent neurons by potassium - induced hyperpolarization . - pubmed
The uridine nucleotide - activated P2Y2 , P2Y4 and P2Y6 receptors are widely expressed in the brain and are involved in many CNS processes , including those which malfunction in Alzheimer ' s disease ( AD ) . - pubmed
These results provide a basis for considering P2Y2 receptor changes as a neurochemical substrate of AD , and point towards uridine nucleotide - activated P2Y receptors as novel targets for disease - modifying AD pharmacotherapeutic strategies . - pubmed
Two short stretches of amino acid sequences at the membrane proximal to the N- and C - termini ( amino acids 61 - 71 and 299 - 303 respectively ) were indispensable for Golgi localization of RKTG , but were not required for the interaction with Raf-1 . - pubmed
LPA binds to selective cell surface receptors to promote cell growth , survival , motility and differentiation . - pubmed
Recent work identifies a secreted enzyme , autotaxin ( ATX ) , as the key component of an extracellular pathway for generation of lysophosphatidic acid by lysophospholipase D catalyzed hydrolysis of lysophospholipid substrates . - pubmed
Accordingly , pharmacological inhibition of ATX may be a viable and potentially effective way to interfere with LPA signaling in the cardiovascular system and possibly other settings such as tumor metastasis for therapeutic benefit . - pubmed
In this review we provide an update on recent advances in defining roles for LPA signaling in major disease processes and discuss recent progress in understanding the regulation and function of autotaxin focusing on strategies for the identification and initial evaluation of small molecule autotaxin inhibitors . - pubmed
The effects of culturing time , and carbon and nitrogen sources on enzyme production were systematically investigated . - pubmed
Stabilisation of electrochemically deposited Prussian blue ( PB ) films on glassy carbon ( GC ) electrodes has been investigated and an enhancement in the stability of the PB films is reported if the electrodes are treated with tetrabutylammonium toluene-4 - sulfonate ( TTS ) in the electrochemical activation step following the electrodeposition . - pubmed
A multi - enzyme PB based biosensor for sucrose detection was made in order to demonstrate that PB films can be coupled with an oxidase system . - pubmed
A tri - enzyme system , comprising glucose oxidase , mutarotase and invertase , was crosslinked with glutaraldehyde and bovine albumin serum on the PB modified glassy carbon electrode . - pubmed
The deposited PB operated as an electrocatalyst for electrochemical reduction of hydrogen peroxide , the final product of the enzyme reaction sequence . - pubmed
The optimal concentrations of the immobilisation mixture was standardised as 8U of glucose oxidase , 8U of mutarotase , 16U of invertase , 0.5 % glutaraldehyde ( 0.025mul ) and 0.5 % BSA ( 0.025 mg ) in a final volume of 5mul applied at the electrode surface ( 0.066cm(2 ) ) . - pubmed
An increase in the operational and storage stability of the sucrose biosensor was also noted when the PB modified electrodes were conditioned in phosphate buffer containing 0.05 M TTS during the preparation of the PB films . - pubmed
AIM : This paper is a report of a study to describe patients ' and nurses ' perspectives on oxygen therapy . - pubmed
We identified specific amino acid propensities at the interfaces of antigen - antibody interactions in non - redundant qualified antigen - antibody complex structures from Protein Data Bank . - pubmed
Propensities were expressed by the frequency of each of the 20 x 20 standard amino acid pairs that appeared at the interfaces of the complexes and were named the antibody - specific epitope propensity ( ASEP ) index . - pubmed
, SLCO1B1 ) , which are potentially responsible for AR-67 carboxylate clearance . - pubmed
We evaluated the concentrations of PDE5 - I , cAMP and cGMP in prostate tissues and plasma , and calculated the prostate tissue - to - plasma ( T / P ) ratio . - pubmed
RESULTS : The concentration of udenafil in prostate tissue and plasma was 2028.6 + - 360.8 ng / g and 463.7 + - 39.1 ng / mL , respectively , and the resulting T / P ratio was 4.4 . - pubmed
Based on a cell - injury model made by ozone ( 1.5 x 10(-6 ) ) exposure , the present study investigated the protective effect of fibronectin ( Fn ) , a kind of ligand of integrin , and it 's specific sequence Arg - Gly - Asp ( RGD peptide ) which is a recognizable domain for integrin on BEC . - pubmed
3H release , LDH and MDA were measured as indexes for damage . - pubmed
RESULTS : ( 1 ) Ozone exposure induced a significant increase of 3H release and LDH release from BEC and elevation of MDA . - pubmed
( 2 ) Pretreatment with Fn or RGD before ozone exposure markedly attenuated the release of 3H , LDH and MDA , and the effects of Fn were reversed by W7 , a calmodulin inhibitor . - pubmed
( 3 ) Either Fn or RGD up - regulated activity of catalase in BEC and promoted GSH synthesis in BEC . - pubmed
Previous experiments at the Rifle , Colorado Integrated Field Research Challenge ( IFRC ) site demonstrated that field - scale addition of acetate to groundwater reduced the ambient soluble uranium concentration . - pubmed
Additionally , ABM induced DNA fragmentation , a characteristic of apoptosis and the expressions of apoptosis - related proteins , including apoptosis - inducing factor , cytochrome c , and caspase-3 , were increased . - pubmed
: The objective of this study was to determine the toxicity of cisplatin - based intraperitoneal ( IP)/intravenous ( IV ) treatment using a modified version of the IP / IV arm of GOG 172 . - pubmed
BACKGROUND : Based principally on findings in three studies , the collaborative reanalysis ( CR ) , the Women 's Health Initiative ( WHI ) and the Million Women Study ( MWS ) , it is claimed that hormone replacement therapy ( HRT ) with estrogen plus progestogen ( E+P ) is now an established cause of breast cancer ; the CR and MWS investigators claim that unopposed estrogen therapy ( ET ) also increases the risk , but to a lesser degree than does E+P. - pubmed
Glutathione S - transferase ( GST ) is a polymorphic supergene family involved in the detoxification of numerous toxins including OCPs . - pubmed
The collected samples at the time of delivery / after delivery were analyzed for OCPs levels by gas chromatography and polymorphic analysis for GSTM1 / GSTT1 gene using multiplex PCR . - pubmed
When interaction between GSTM1 / GSTT1 genes polymorphism - OCPs levels and birth weight ( gene - environment interaction ) was ascertained , a significant association was seen between beta - HCH and GSTM1- genotype with reduction in birth weight of 213 g. CONCLUSION : Higher levels of OCPs in pregnant women may be considered as an important aetiological factor in ' idiopathic ' FGR . - pubmed
Evaluation of double - disk potentiation and disk potentiation tests using dipicolinic acid for detection of metallo - beta - lactamase - producing pseudomonas spp . - pubmed
A high false - positive rate is a problem with EDTA - based MBL detection methods . - pubmed
aeruginosa isolates with false - negative DDPTs with MEM - DPA 250 - mug disks carried bla(IMP-6 , ) and the high level resistance to MEM ( MIC & gt;= 512 mug / ml ) was reduced by the presence of phenylalanine arginine beta - naphtylamide . - pubmed
In DPA DDPTs , IPM disks perform better than MEM disks when the isolates are highly resistant to MEM due to the overexpression of efflux pumps . - pubmed
In the present study , we have developed an osteoinductive coating of a porous titanium implant using biomimetic polyelectrolyte multilayer ( PEM ) films loaded with BMP-2 . - pubmed
BMP-2 loaded in dried PEM films exhibited shelf - life stability over one year . - pubmed
Remarkably , the in vivo studies showed that the osteoinductive potential of BMP-2 contained in PEM - coated Ti implants was fully preserved after air - drying of the implants and sterilization at 25 kGy . - pubmed
We report a real - space visualization of the formation of hydrogen bonding in 8 - hydroxyquinoline ( 8 - hq ) molecular assemblies on a Cu ( 111 ) substrate , using noncontact atomic force microscopy ( NC - AFM ) . - pubmed
Intermolecular coordination between the dehydrogenated 8 - hq and Cu adatoms was also revealed by the submolecular resolution AFM characterization . - pubmed
Cell organelles from crassulacean acid metabolism ( CAM ) plants : II . - pubmed
After separation of cell organelles by isopycnic centrifugation , enzymes of the Crassulacean acid metabolism were found in the following cell fractions : Phosphoenolpyruvate carboxylase in the chloroplasts ; NAD - dependent malate dehydrogenase in the mitochondria and in the supernatant ; NADP - dependent malate dehydrogenase and phosphoenolpyruvate carboxykinase in the chloroplasts ; NADP - dependent malic enzyme in the supernatant and to a minor extent in the chloroplasts ; NAD - dependent malic enzyme in the supernatant and to some degree in the mitochondria ; and pyruvate ; orthophosphate dikinase in the chloroplasts . - pubmed
The activity of the NAD - dependent malate dehydrogenase was due to three isoenzymes separated by ( NH4 ) 2SO4 gradient solubilization . - pubmed
It was previously demonstrated that delivery of exogenous wild - type tumor suppressor gene p53 via a tumor - targeted nanocomplex ( SGT-53 ) which crosses the blood - brain barrier could sensitize highly TMZ - resistant GBM tumors to TMZ . - pubmed
Although metastable from 0 to 50 GPa , the new phase is more stable at low pressures than the other well - known HCP and FCC allotropes and smoothly transforms into the FCC phase under compression . - pubmed
Burukutu consumption at 0.78 g / kg alcohol produced no significant change in the activities of alkaline phosphatase ( ALP ) , alanine aminotransferase ( ALT ) , and aspartate aminotransferase ( AST ) . - pubmed
Subsequently , flow cytometry was performed to investigate indomethacin - induced apoptosis following the overexpression or knockdown of Smac , and western blot analysis was performed to determine the expression of Smac and the activation of caspase-3 . - pubmed
Western blot analysis demonstrated that indomethacin induced apoptosis through releasing Smac into the cytosol and activating caspase-3 . - pubmed
These results indicated that Smac is essential for the apoptosis induced by indomethacin in esophageal cancer cells . - pubmed
Crystal structure analysis of extrinsic PsbP protein of photosystem II reveals a manganese - induced conformational change . - pubmed
Conversely , the introduction of bromine at C7 of the 2,4 - dichloro [ 3,2 - d ] pyrimidine eliminated cell type - dependent differences in cytotoxicity or cell cycle status . - pubmed
Together , these data indicate that a substituent at C7 can profoundly modify the cytotoxic mechanism of halogenated thieno [ 3,2 - d ] pyrimidines in a cell type - specific manner . - pubmed
The results demonstrated that ADSC transplantation reduced the serum levels of alanine aminotransferase , total bilirubin , total cholesterol , triglycerides and fatty acids , and reduced the content of malondialdehyde in the liver homogenates . - pubmed
If used to estimate the presence of low rivaroxaban concentrations , POCT was superior to predictions based on normal prothrombin time and activated partial thromboplastin time values even if sensitive reagents were used . - pubmed
The linear response ranges were 0.3 - 20000nM for celecoxib , and 1.2 - 20000nM , 0.3 - 20000nM , 2.0 - 2000nM , 1.5 - 6000nM for its metabolites carboxycelecoxib ( M2 ) , hydroxycelecoxib ( M3 ) , hydroxycelecoxib glucuronide ( M1 ) , and carboxycelecoxib glucuronide ( M5 ) , respectively . - pubmed
In conclusion , our results showed that our validated LC - MS / MS method can be successfully used for the pharmacokinetic studies of celecoxib and that the blood cells are a very important compartment for this drug such that profiles of celecoxib and its metabolites in whole blood will be more comprehensive and accurate representation of their profiles in vivo than the plasma . - pubmed
Herein , a novel red - emitting LPL phosphor Ca3Ti2O7 : Pr ( 3 + ) is successfully prepared by a high - temperature solid - state method . - pubmed
Finally , a possible LPL mechanism for Ca3Ti2O7 : Pr ( 3 + ) is proposed . - pubmed
Over 73 fluorinated proteins have been studied by ( 19 ) F NMR since the seminal studies of Hull and Sykes in 1974 . - pubmed
With advances in cryoprobe production and fluorinated amino acid incorporation strategies , protein - based ( 19 ) F NMR offers opportunities to the medicinal chemist for characterizing and ultimately discovering new small molecule protein ligands . - pubmed
ABBREVIATIONS : CML = Chronic Myeloid Leukemia , BCR - ABL1 = Break Cluster Region - Abelson gene , TKI = tyrosine kinase inhibitor treatment , ACAs = additional cytogenetic abnormalities , CCyR = complete cytogenetic response , PCyR = partial cytogenetic response , mCyR = minor cytogenetic response , MMR = major molecular response , HSCT = hematopoietic stem cell transplant , HLA = human leukocyte antigens , CP = chronic phase , AP = accelerated phase , BP = blast phase , OS = overall survival , CBA = chromosome banding analysis , +8 = trisomy 8 , i(17q ) = isochromosome ( 17q ) , + Ph = second Philadelphia chromosome , -7 = monosomy 7 , -17 = monosomy 17 , +17 = trisomy 17 , -21 = monosomy 21 , +21 = trisomy 21 , -Y = loss of Y chromosome , ELN = European LeukemiaNet , IMA600 = Imatinib 600 mg daily , IMA400 = Imatinib 400 mg daily , NILO600 = Nilotinib 600 mg daily , DASA100 = Dasatinib 100 mg daily , DASA140 = Dasatinib 140 mg daily . - pubmed
All - trans retinoic acid modulates Wnt3A - induced osteogenic differentiation of mesenchymal stem cells via activating the PI3K / AKT / GSK3beta signalling pathway . - pubmed
Here , we explored whether Wnt3A and all - trans - retinoic acid ( ATRA ) play synergistic roles in MSC osteogenic differentiation . - pubmed
We found that ATRA potentiated the Wnt3A - induced expression of early and late osteogenic markers as well as matrix mineralization and further confirmed the phenomena using foetal limb explant culture and MSC implantation experiments . - pubmed
Mechanistically , ATRA cooperated with Wnt3A to induce beta - catenin translocation from cell - cell contacts into the cytosol and nucleus , thereby activating Wnt / beta - catenin signalling . - pubmed
Additionally , Wnt3A attenuated ATRA - induced Cyp26a1 expression , inhibiting the degradation of ATRA into its oxidative forms . - pubmed
beta - catenin silencing abolished the stimulatory effect of ATRA on Wnt3A - induced alkaline phosphatase ( ALP ) activity and reversed its inhibitory effect on Cyp26a1 expression . - pubmed
Furthermore , ATRA and Wnt3A synergistically promoted AKT phosphorylation , enhancing beta - catenin - dependent transcription through GSK3beta inhibition or direct beta - catenin phosphorylation at Ser552 . - pubmed
This event was largely abolished by LY294002 pre - treatment , suggesting that ATRA and Wnt3A at least partially promote osteogenic differentiation via activating the PI3K / AKT / GSK3beta signalling pathway . - pubmed
Thus , crosstalk between the Wnt / beta - catenin and retinoic acid signalling pathways may be an effective therapeutic target for bone diseases , such as osteoporosis . - pubmed
MAIN OUTCOMES AND MEASURES : Seventeen performance measures related to diabetes mellitus ( including hemoglobin A1c control , retinal eye examination , low - density lipoprotein cholesterol control , nephropathy screening , and blood pressure control ) , cardiovascular disease ( including low - density lipoprotein cholesterol control , blood pressure control , and use of a beta - blocker after myocardial infarction ) , cancer screening ( colorectal and breast ) , and appropriate medications ( including systemic corticosteroids and bronchodilators for chronic obstructive pulmonary disease [ COPD ] and disease - modifying antirheumatic drugs ) . - pubmed
The association with adolescent fruit intake was stronger for both estrogen and progesterone receptor negative cancers than estrogen and progesterone receptor positive cancers ( P=0.02 for heterogeneity ) . - pubmed
Biological variation of glucose , insulin and lipids in lean , insulin - sensitive and obese , insulin - resistant Chinese males without diabetes . - pubmed
The CB1 Neutral Antagonist AM4113 Retains the Therapeutic Efficacy of the Inverse Agonist Rimonabant for Nicotine Dependence and Weight Loss with Better Psychiatric Tolerability . - pubmed
Rimonabant , a CB1 inverse agonist found to be effective for smoking cessation , was associated with an increased risk of anxiety and depression . - pubmed
In contrast to previously identified azole - resistant isolates related to cyp51A mutations , only one isolate carries a point mutation in cyp51A ( F219L mutation ) among 105 independent stable azole - resistant isolates . - pubmed
High - performance liquid chromatography ( HPLC ) analysis verified that the decreased absorption of itraconazole in related Afcox10 mutants is the primary reason for itraconazole resistance . - pubmed
Moreover , a complementation experiment by reengineering the mutation in a parental wild - type background strain demonstrated that both the F219L and R243Q mutations contribute to itraconazole resistance in an algA - independent manner . - pubmed
These data collectively suggest that the loss of algA results in an increased frequency of azole - resistant isolates with a non - cyp51A mutation . - pubmed
Clinical and immunological efficiency of ingavirin prevention of recurrent herpetic stomatitis is proved by estimation of oral cavity local immunity ( SlgA , lisozyme ) , humoral immunity ( IgE and IgG ) and cellular immunity ( RBTL with FGA , defined T - lymphocytes ) . - pubmed
We hypothesize that DATS reduces diabetic MI / R injury by activating SIRT1 signaling . - pubmed
We found that DATS treatment markedly improved left ventricular systolic pressure and the first derivative of left ventricular pressure , reduced myocardial infarct size as well as serum creatine kinase and lactate dehydrogenase activities . - pubmed
Furthermore , the myocardial apoptosis was also suppressed by DATS as evidenced by reduced apoptotic index and cleaved caspase-3 expression . - pubmed
However , these effects were abolished by EX527 ( the inhibitor of SIRT1 signaling , 5 mg / kg ) . - pubmed
We further found that DATS effectively upregulated SIRT1 expression and its nuclear distribution . - pubmed
Additionally , PERK / eIF2alpha / ATF4 / CHOP - mediated ER stress - induced apoptosis was suppressed by DATS treatment . - pubmed
Moreover , DATS significantly activated Nrf-2 / HO-1 antioxidant signaling pathway , thus reducing Nox-2 / 4 expressions . - pubmed
Taken together , these data suggest that perioperative DATS treatment effectively ameliorates MI / R injury in type 1 diabetic setting by enhancing cardiac SIRT1 signaling . - pubmed
Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro . - pubmed
The present results showed that chronic DEX exposure significantly increased LDH release and apoptosis , decreased MAP2 and GR expression in hippocampal neurons . - pubmed
DEX ( 5muMu ) exposure for 3d significantly increased the expression of NLRP-1 , ASC , caspase-1 and IL-1beta in the hippocampal neurons and the release of IL-1beta and IL-18 in the supernatants . - pubmed
Moreover , DEX ( 1 , 5muMu ) treatment for 3d significantly increased the expression of NF - kappaB in hippocampal neurons . - pubmed
The GR antagonist , mifepristone ( RU486 ) , had protective effects on chronic DEX induced hippocampal neurons injury and NLRP1 inflammasome activation . - pubmed
The carbapenem resistance mechanisms such as carbapenemase genes , efflux pump hyperexpression , AmpC overproduction , and OprD gene downregulation were determined by phenotypic and molecular methods . - pubmed
Eighteen metallo - beta - lactamase ( MBL ) producer isolates were found to be sensitive to amikacin . - pubmed
The clonal distribution of the strains was related to carbapenem resistance mechanisms ( most of MBL producers belong to the same clones ) and the same hospital wards where the isolates were collected . - pubmed
Amikacin was considered a very efficient antibiotic to treatment of MBL - producing CRPA isolates . - pubmed
Our results showed that OprD downregulation and IMP - type MBL are the main carbapenem resistance mechanisms in CRPA isolates from northwest of Iran . - pubmed
Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH / POX - Dependent Pro - Apoptotic Pathways in a Novel in Vitro Cell Culture Model . - pubmed
BACKGROUND / AIMS : The effect of impaired intracellular proline availability for proline dehydrogenase / proline oxidase ( PRODH / POX)-dependent apoptosis was studied . - pubmed
We have used inhibitor of proline utilization in collagen biosynthesis , 2 - metoxyestradiol ( MOE ) , inhibitor of prolidase that generate proline , rapamycin ( Rap ) and glycyl - proline ( GlyPro ) , substrate for prolidase . - pubmed
The optimized LC - MS / MS method described here has been utilized extensively for metabolomics analysis of polar metabolites . - pubmed
T-614 Promotes Osteoblastic Cell Differentiation by Increasing Dlx5 Expression and Regulating the Activation of p38 and NF - kappaB. - pubmed
The contents and activity of alkaline phosphatase , levels of collagen type I and bone gla protein , and calcium nodule formation were increased significantly after T-614 treated . - pubmed
The results showed that T-614 promotes osteoblastic differentiation by increasing the expression of Osterix and Dlx5 and increasing the activation of P38 . - pubmed
T-614 could advance the ectopic expression of NF - kappaB to suppress inflammation , which indirectly inhibits the damage of the osteoblasts . - pubmed
ArMR displayed the optimum catalytic activity at 50 C , pH 7.8 in Tris - HCl buffer . - pubmed
KM parameter of ArMR towards ( R )- and ( S )- mandelic acid was 1.44 and 0.81 mmol / L , respectively ; the corresponding kcat value was 410 s-1 and 218 s-1 . - pubmed
In addition , KM of ArMR towards ( R )- and ( S )- 2 - chloro mandelic acid was 6.48 and 6.37 mmol / L , and the corresponding kcat value 0.22 s-1 and 0.23 s-1 , respectively . - pubmed
Meanwhile , Mg2 + and Mn2 + could activate the enzyme whereas Zn2 + inactivated the enzyme completely . - pubmed
The results revealed that LBP protected the proliferative ability of trophoblast cells injured with H2O2 in a dose - dependent manner . - pubmed
LBP inhibited the oxidative stress induced by H2O2 , by reducing ROS and LDH levels and increasing SOD activity . - pubmed
Additionally , LBP decreased MMP disruption and cell apoptosis induced by H2O2 , by increasing the mRNA and protein expression of survivin , HIF1 - alpha and Bcl-2 and decreasing Bax expression . - pubmed
Presynaptic Ca2 + accumulation in the terminal was also enhanced in DRP1 - preKO synapses . - pubmed
Synaptic transmission defects could be partially rescued with EGTA - AM , indicating close coupling of Ca2 + channels to SV distance normally found in mature terminals may be compromised by DRP1 - preKO . - pubmed
The characterization studies of these derivatives were carried by using different spectroscopic techniques ( 1H NMR , 13C NMR and MS ) and elemental analyses . - pubmed
Vemurafenib , a selective BRAF kinase inhibitor , has been found to induce several cutaneous adverse effects , ranging from a keratosis pilaris - like reaction to squamous cell carcinoma . - pubmed
Abbreviations : BM = bone metastases ; COX = multivariate analyses ; DM = distant metastases ; DSM = disease - specific mortality ; DSS = disease - specific survival ; DTC = differentiated thyroid carcinoma ; ETE = extrathyroidal extension ; LNM = lymph node metastases ; OM = overall mortality ; OS = overall survival ; PTCFV = papillary thyroid carcinoma ; RAI = radioactive iodine ; SM = spinal metastases ; SRE = skeletal - related event ; txWBS = whole - body scan after RAI therapy . - pubmed
Hepcidin is a main regulator of iron metabolism , of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of iron by interacting with ferroportin . - pubmed
Alborixin clears amyloid - beta by inducing autophagy through PTEN - mediated inhibition of the AKT pathway . - pubmed
We found that autophagy induced by alborixin substantially cleared Abeta in microglia and primary neuronal cells . - pubmed
Autophagy induced by alborixin was associated with inhibition of the phosphoinositide 3 - kinase ( PI3K)-AKT pathway . - pubmed
A knock down of PTEN and consistent , constitutive activation of AKT inhibited alborixin - induced autophagy and consequent clearance of Abeta . - pubmed
Abbreviations : Abeta : amyloid beta ; ALB : alborixin ; ATG : autophagy - related ; BECN1 : beclin 1 ; DAPI : 4 , 6 - diamidino-2 - phenylindole ; DCFH - DA : 2,7 - dichlorodihydrofluorescein diacetate ; fAbeta : fibrillary form of amyloid beta ; GFAP : glial fibrillary acidic protein ; MAP1LC3B / LC3B : microtubule - associated protein 1 light chain 3 beta ; MAP2 : microtubule - associated protein 2 ; MTOR : mechanistic target of rapamycin kinase ; PTEN : phosphatase and tensin homolog ; ROS : reactive oxygen species ; SQSTM1 : sequestosome 1 ; TMRE : tetramethylrhodamine , ethyl ester . - pubmed
BACKGROUND : Previous research demonstrates increased utilization of high - intensity statins , but unchanged low - density lipoprotein cholesterol ( LDL - C ) levels , immediately after the 2013 American College of Cardiology ( ACC ) and the American Heart Association ( AHA ) guideline release . - pubmed
Atomic force microscopy ( AFM ) normal force measurements provide insight into the architecture of the HA / LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of LUB end - domains directly to HA chains . - pubmed
These AFM force measurements indicate that the adhesion of LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules . - pubmed
Prmt7 promotes myoblast differentiation via methylation of p38MAPK on arginine residue 70 . - pubmed
The mechanistic study reveals that Prmt7 methylates p38MAPKalpha at the arginine residue 70 , thereby promoting its activation which in turn enhances MyoD activities . - pubmed
The arginine residue 70 to alanine mutation in p38MAPKalpha impedes MyoD / E47 heterodimerization and the recruitment of Prmt7 , MyoD and Baf60c to the Myogenin promoter resulting in blunted Myogenin expression . - pubmed
In conclusion , Prmt7 promotes MyoD - mediated myoblast differentiation through methylation of p38MAPKalpha at arginine residue 70 . - pubmed
PANF - ATL was characterized by elemental analysis , FTIR , TGA , SEM and XPS . - pubmed
The transfer factor ( TF ) and the total bioaccumulation amount ( TBA ) of uranium were used to describe remediation efficiencies in this paper . - pubmed
Results indicated that sperm motility was significantly reduced from 1 day following TiNP injection ( in both dose ) , and this reduction persisted up to 9 days post - TiNP injection ( 10 mg / kg injection group ) . - pubmed
The LC sensor was fabricated by immobilizing 3 microg mL-1 BSA solution on dimethyloctadecyl -[ 3 -( trimethoxysilyl ) propyl ] ammonium chloride ( DMOAP)-coated glass slides . - pubmed
Three - point bending tests showed higher mechanical properties for PUF - DCPD ( 80 % ) than MUF - ENB ( 40 % ) compared to the neat epoxy systems . - pubmed
Serum 25 - hydroxyvitamin D ( 25 ( OH ) D ) levels were determined by radioimmunoassay and liver and adipose tissue cholecalciferol ( VD3 ) and 25 - hydrocholecalciferol ( 25 ( OH ) D3 ) levels were measured by LC - MS / MS . - pubmed
There was no difference in mRNA levels of liver 25 - hydroxylases by both dietary fat amount and vitamin D content . - pubmed
Although the total cholesterol values were normal , the very - low - density lipoprotein of cholesterol level was high ( 49 + - 7 mg / dL ) . - pubmed
The mean values of total triglyceride , very - low - density lipoprotein of both triglyceride and cholesterol decreased significantly after the treatment period ( 319 + - 29 , 245 + - 18 , and 27 + - 2 mg / dL , respectively , P < 0.05 ) . - pubmed
The addition of Ag+ triggered the self - assembly of branched PNAs through the formation of C - Ag +- C metallo base pairing of the three PNA C4 arms leading to non - covalent dendrimers , whose architecture is directed by the C4 ( R / S )-stereocenter of core 4 - aminoproline . - pubmed
Post - ban samples were asked about awareness of the ban and access to menthol cigarettes . - pubmed
However , odds were lower post - ban for reporting menthol as the usual cigarette ( OR=0.80 , 95 % CI 0.72 to 0.90 ) , and for smoking only menthol cigarettes ( OR=0.19 , 95 % CI 0.18 to 0.19 ) . - pubmed
Moreover , Cm3639 and Cm3769 can convert the GlcNAc dimer and colloidal chitin ( CC ) into GlcNAc , which showed that they also possess NAGase activity . - pubmed
In addition , NAGase Cm3245 possesses a very high exo - acting activity of hydrolyzing GlcNAc dimer . - pubmed
These results suggest that chitinases and NAGase from SYBC - H1 both play important roles in conversion of N - acetyl chitooligosaccharides to GlcNAc , resulting in the accumulation of the final product GlcNAc . - pubmed
The intravenous injection of glucose as a " therapeutic test " in comatose diabetics is reviewed in the light of evidence that it may evoke a paradoxical rise in plasma - potassium in the insulin - deprived diabetic . - pubmed
The plasma - potassium rose promptly after a drink of 50 g glucose in 8 insulin - dependent diabetics who had not had insulin for approximately 14 - 26 hours . - pubmed
Initially , the microsomal mixed function oxidase system was assumed to be capable of oxidizing ethanol in a mechanism that did not involve either alcohol dehydrogenase or catalase . - pubmed
Later evidence suggested that the oxidative enzyme was , in fact , catalase , a contaminant of microsomal preparations and that the mixed function oxidase system merely furnished hydrogen peroxide to the reaction . - pubmed
The actin content of fibroblasts may be low or the amino acid sequences of muscle and fibroblast actin may differ in the N - terminal region . - pubmed
Lectin affinity chromatography of proteins bearing O - linked oligosaccharides : application of jacalin - agarose . - pubmed
These proteins were eluted from columns of jacalin - agarose specifically by alpha - galactopyranosides such as melibiose and alpha - methylgalactopyranoside but not by lactose or other sugars . - pubmed
Treatment of asialofetuin with endo -- alpha -- N -- acetylgalactosaminidase eliminated its affinity for the lectin column , and other proteins known to contain only N - linked oligosaccharides such as ovalbumin , transferrin , and alpha 1 - acid glycoprotein were not retained by the lectin . - pubmed
Binding of proteins with O - linked oligosaccharides to the lectin column did not require divalent cations and was affected little by changes in pH and ionic strength over a wide range . - pubmed
Virtually all of the glycosidically linked oligosaccharides of fetuin , chorionic gonadotropin , and plasminogen are known to be sialated . - pubmed
Thus , binding of these glycoproteins to jacalin , which is known to have affinity for the core disaccharide , 1 - beta - galactopyranosyl-3 -( alpha-2 - acetamido-2 - deoxygalactopyranoside ) , in O - linked oligosaccharides of these proteins , was not prevented by the presence of sialic acids . - pubmed
Affinity of oligosaccharides for jacalin did appear to be reduced by occurrence of sialic acids as it was found that higher concentrations of melibiose were required to elute asialofetuin than fetuin from jacalin - agarose . - pubmed
Results of the present study indicate that affinity chromatography using this lectin is a widely applicable technique for identifying and purifying proteins bearing O - linked oligosaccharides . - pubmed
Several polypeptide mediators capable of stimulating fibroblasts replication or excessive collagen deposition have been implicated in this , but the precise role of these in bleomycin - induced fibrosis is yet to be demonstrated . - pubmed
The beta - turn formed by the amino acid residues 20 - 23 of the B - chain of insulin has been implicated as an important structural feature of the molecule . - pubmed
In two analogues , we have substituted alpha - aminoisobutyric acid ( Aib ) for the Glu residue normally present in position B21 or for the Arg residue normally present in position B22 ; in a third compound , we have replaced the Glu residue with its D - isomer . - pubmed
D -[ U-14C ] Glucose tracer exchange , measured under voltage - clamp condition at Na+/D - glucose equilibrium , revealed that addition of calcitriol to the culture medium approximately doubled the activity of the Na +/ D - glucose transporter in the brush - border membrane . - pubmed
Further , a model including age , smoking habits , fibrinogen , viscosity , and white blood cell count predicts IHD as well as one in which the three hemostatic / rheological variables are replaced by total cholesterol , diastolic pressure , and body mass index . - pubmed
Because Ca2 + has been implicated as a regulator of mitogenic processes in a number of tissues , we investigated whether GH affects the transmembrane movement of Ca2 + through voltage - activated channels of cardiac myocytes . - pubmed
The results suggest that a tumor - derived growth factor , most likely GH , can cause a specific enhancement of T - type Ca2 + current in atrial myocytes . - pubmed
Neither the plasma insulin response to meals nor insulin - stimulated glucose uptake improved with acarbose therapy , consistent with the view that acarbose improves glycemic control by delaying glucose absorption . - pubmed
Ga - labeling of immunoglobulin G with high specific radioactivity . - pubmed
To obtain radiogallium labeled immunoglobulin G with a high specific radioactivity for in vitro use , a 67Ga source was purified by extraction from 67Ga - gallium citrate with butyl acetate , and a 67Ga - labeling solution was produced . - pubmed
The adenosine - stimulated increase in vascularity was blocked with 110 micrograms of methyl - isobutyl - xanthine injected daily into the albumin . - pubmed
Compounds I and II were also formed from the reaction of 1.0 microM 20 - OH - LTB4 , PMN microsomes , and 100 microM NADPH ; the identity of compound II was confirmed as 20 - COOH - LTB4 by gas chromatography - mass spectrometry . - pubmed
Addition of immune IgG to cytochrome P-450 reductase or 1.0 mM SKF-525A completely inhibited the formation of 20 - CHO - LTB4 from 20 - OH - LTB4 , indicating that the reaction was catalyzed by a cytochrome P-450 . - pubmed
LTB5 ( 3.0 microM ) , a known substrate for cytochrome P-450LTB and a competitive inhibitor of LTB4 omega - oxidation , completely inhibited the omega - oxidation of 1.5 microM 20 - OH - LTB4 to 20 - CHO - LTB4 , indicating that the cytochrome P-450 was P-450LTB . - pubmed
Conversion of 1.0 microM 20 - CHO - LTB4 to 20 - COOH - LTB4 by PMN microsomes was also dependent on NADPH and inhibited by antibody to cytochrome P-450 reductase , 1.0 mM SKF-525A , or 5.0 microM LTB5 , indicating that this reaction was also catalyzed by cytochrome P-450LTB . - pubmed
These results identify the novel metabolite 20 - CHO - LTB4 and indicate that cytochrome P-450LTB catalyzes three sequential omega - oxidations of LTB4 leading to the formation of 20 - COOH - LTB4 via 20 - OH - LTB4 and 20 - CHO - LTB4 intermediates . - pubmed
By comparing inactivating effects of diethyldithiocarbamic acid mixed disulfides with thiols of increasing chain length evidence is provided that steric hindrance is the reason for lack of inhibition of the mitochondrial enzyme by disulfiram in vitro . - pubmed
52 , 187 - 222 ] the results also suggest a mechanism by which aldehyde dehydrogenase is inhibited by disulfiram and diethyldithiocarbamic acid in vivo . - pubmed
Lymphocyte functions , such as PHA response , surface marker and serum IAP , before and after NCS injections were the same . - pubmed
Epidermal growth factor receptor expression during morphological differentiation of pheochromocytoma cells , induced by nerve growth factor or dibutyryl cyclic AMP . - pubmed
In addition , exposure of the cells to dbcAMP caused a twofold increase of EGF - receptor phosphotyrosine kinase activity . - pubmed
It is shown that PRO induces mutants by means of " mutation - mitotic segregation " mechanism , while HAP induces mutants through novel mechanism " both allele mutation " . - pubmed
We used phosphate buffer with Triton X-100 for extracting apoB on DBS . - pubmed
While there was little effect of most of the native preparations tested , some batches showed significant release of histamine or lysosomal enzymes after heating to 70 degrees C. HSA preparations produced by heat ethanol fractionation never induced significant release reactions . - pubmed
Oxidation of glutathione by the myeloperoxidase system . - pubmed
Oxidation of glutathione ( GSH ) by the myeloperoxidase ( MPO ) system was studied . - pubmed
The combination of MPO , H2O2 , and a halide ion oxidized GSH . - pubmed
The MPO - mediated oxidation of GSH required the simultaneous presence of MPO , H2O2 , and a halide ion . - pubmed
The oxidative product was shown to be GSSG , since it could be reduced back to GSH by glutathione reductase and NADPH . - pubmed
The MPO - mediated oxidation of GSH may be one mechanism by which this system damages microorganisms . - pubmed
Psoralens were placed specifically near the BamHI site , in the tetracycline - resistance gene of pBR322 , using a sulfhydryl - containing psoralen derivative . - pubmed
Morphine analgesia potentiated but tolerance not affected by active immunization against cholecystokinin . - pubmed
This process goes from immature ( To ) to semi - mature ( T1 ) and mature ( T2 ) lymphocytes , these latter mostly characterized by the absence of TdT and by the property to form E - rosettes with SRBC and respond to mitogens . - pubmed
These mitochondria have a large capacity to flux pyruvate through the pyruvate carboxylase reaction causing an accumulation of malate , citrate and phosphoenolpyruvate . - pubmed
As much as 70 % of the pyruvate utilized during state 3 respiration can flux through the pyruvate carboxylase reaction . - pubmed
The reactivity of Ab1 and Ab3 with two amantadine - resistant virus mutants indicates that IIF4 epitope ( and its related region recognized by Ab3 ) becomes accessible in consequence of destabilization of trimeric arrangement of HA and it also correlates with expulsion of N - terminus of HA2 . - pubmed
The routinely used immunoenzyme peroxidase ( PO ) labelling has been replaced by alkaline phosphatase ( AP ) using new fuchsin as substrate . - pubmed
The enzyme was also active against alpha - fructosyl lysine and borohydride - reduced Amadori product , weakly active with ribated and glycated polylysine , and inactive with reducing sugars , amino acids , and glycated proteins . - pubmed
The reaction products were characterized by 1H NMR , 13C NMR , and GC / MS and found to correspond to 1 - deoxy-1 - aminofructose , i.e. - pubmed
In contrast , however , the new enzyme acts on the alkylamine bond rather than the ketoamine bond of the Amadori product . - pubmed
Postexercise elevations in serum cortisol and prolactin were seen only in the male subjects . - pubmed
For serum testosterone , neither resting nor higher post - exercise concentrations were different at any point of the training periods for either sex . - pubmed
Relationship between plasma triglycerides and apolipoprotein CII in infants during the first year of life . - pubmed
Plasma triglyceride and apolipoprotein CII ( apo CII ) levels were measured in normal full - term infants and low - birth - weight infants at birth ( from umbilical cord blood at 0 weeks ) , as well as at 2 , 3 , 4 , 8 , 24 , and 48 weeks of age , and the relationship between plasma triglyceride and apo CII levels was studied . - pubmed
In both full - term and low - birth - weight infants , the plasma triglyceride and apoprotein CII levels were very low at birth , but they increased rapidly thereafter in the full - term infants and somewhat more slowly in the low - birth - weight infants . - pubmed
Both triglyceride and apoprotein CII levels became stable from 3 weeks of age in full - term infants and from 8 weeks in low - birth - weight infants . - pubmed
Characterization of HIV1 - PAR , a macrophage - tropic strain : cell tropism , virus / cell entry and nucleotide sequence of the envelope glycoprotein . - pubmed
Five CD4 - binding domains , the gp120 / gp41 cleavage site , the putative gp41 fusion domain and 21 out of the 22 cysteine residues were conserved in both isolates . - pubmed
Competition binding studies using AH 6809 ( EP1 antagonist ) , butaprost ( EP2 agonist ) , M/+B 28,767 ( EP3 agonist ) , and AH 23848B ( EP4 antagonist ) suggested that the retinal vessels of the newborn contained approximately equal number of EP1 and EP2 receptor subtypes whereas the main receptor subtype in the adult vessels was EP1 . - pubmed
PGE2 , 17 - phenyl trinor PGE2 ( EP1 agonist ) and PGF2 alpha caused a 2.5 to 3 - fold greater increase in inositol 1,4,5 - triphosphate ( IP3 ) formation in adult than in newborn preparations . - pubmed
Two of 5 transformants obtained as bialaphos - resistant colonies showed two to twenty times higher specific activity of GUS than the recipient . - pubmed
Based on this finding , scanning mutagenesis was used to find a single - cysteine mutant of the pore - forming protein staphylococcal alpha - hemolysin ( alpha HL ) suitable for caging . - pubmed
A post - capillary reactor is constructed to allow on - line addition of fragment B ( of protein A)-fluorescein to form affinity complexes with separated IgG subclasses . - pubmed
Ethanol disrupts signal transduction mediated by a variety of G - protein coupled receptors . - pubmed
We examined the effects of ethanol on arachidonic acid release mediated by muscarinic acetylcholine receptors . - pubmed
Dose response curves suggest that ethanol acts as a noncompetitive inhibitor of muscarinic receptor - induced [ 3H]AA release insofar as maximal [ 3H ] AA release was depressed in the presence of ethanol with no apparent change in the EC50 for stimulation by carbamylcholine . - pubmed
These results indicate that ethanol acutely inhibits muscarinic receptor signaling through the arachidonic acid pathway in a noncompetitive manner , but chronically enhances muscarinic signaling through the same pathway . - pubmed
The ATPase activity was inhibited by NO3 - and SCN- which are the inhibitors of V - type ATPase , but not by azide and vanadate , inhibitors of F - type ATPase and P - type ATPase , respectively . - pubmed
PCR - based analysis of the freshly harvested tumor from patient PM2 - B2 revealed the presence of message for the melanoma - associated gene products MAGE-1 and MAGE-3 , but not for tyrosinase or MART-1 / MELAN - A. - pubmed
8 M KCl , which contained transcription factor IID ( TFIID ) , overcame the inhibition . - pubmed
BACKGROUND : Deep hypothermic circulatory arrest ( DHCA ) has been shown to cause impairment in recovery of cerebral blood flow ( CBF ) and cerebral metabolism ( CMRO2 ) proportional to the duration of the DHCA period . - pubmed
This effect on CMRO2 may be a marker for brain injury , because CMRO2 recovers normally after cardiopulmonary bypass ( CPB ) when DHCA is not used . - pubmed
The aim of this study was to investigate the effects of intermittent perfusion during DHCA on the recovery of CMRO2 after CPB and to correlate these findings with electron microscopy ( EM ) of the cerebral microcirculatory bed . - pubmed
RESULTS : UI - DHCA resulted in significant impairment to recovery of CMRO2 after CPB ( p & lt ; 0.05 ) . - pubmed
Thus , the co - existence of NADPH - d and AChE occurred in the location of interneurons . - pubmed
Radiolabeled derivatives of WAY-100635 have been shown to be important for imaging in vivo because of their antagonist properties and their specificity for the 5 - hydroxytryptamine ( 1A ) ( 5 - HT ( 1A ) ) receptor . - pubmed
He was administered benzinpenicilline , chloramphenicol - succinate , lanatoside , human albumins , dexamethasone .... - pubmed
Statins , 3 - hydroxy-3 - methylglutaryl coenzyme A ( HMG - CoA ) reductase inhibitors , are a class of drugs with a potent lipid - lowering effect that have been shown to reduce LDL cholesterol by 35 - 45 % at therapeutic doses . - pubmed
At present , the primary target of statins is LDL cholesterol reduction , but with additional effects on the inflammatory cell of the plaque with a reduction in macrophages and secretion of collagenolytic metalloproteases and reinforcing the fibrous skeleton of the plaque by increasing the content of interstitial collagen . - pubmed
The increased serum adrenocorticotropic hormone , corticosterone and serotonin levels observed following melatonin and p - chloroamphetamine treatment suggest that this combination may have adverse peripheral effects . - pubmed
Nitric oxide ( NO ) regulates cyclo - oxygenase ( COX ) activity in various cell systems and reports conflict in regard to its stimulatory versus inhibitory role . - pubmed
The COX-2 induction was dependent on a de novo synthesis since cycloheximide , an inhibitor of protein synthesis , blocked the enzyme expression . - pubmed
The increase in COX-2 expression was not accompanied by a corresponding change in prostaglandin ( PG ) production . - pubmed
However , the COX activity was partially recovered when immunoprecipitated COX-2 was incubated with arachidonic acid and haematin . - pubmed
Peroxynitrite , a highly reactive nitrogen molecule derived from the interaction of NO and superoxide anion , significantly increased COX-2 expression . - pubmed
Under these conditions and within the limit of detection of the antibody , selective antibody for nitrotyrosine failed to detect nitrated COX-2 in immunoprecipitated COX-2 when cells where incubated with SIN-1 or SIN-1+IL-1alpha . - pubmed
Ro 31 - 8220 , a specific inhibitor of protein kinase ( PK ) C , blocked the induction of COX-2 . - pubmed
Also , SB203580 , the selective inhibitor of p38 MAP kinase , strongly blocked the induction of COX-2 by SIN-1 in the presence or absence of IL-1alpha , whereas the MEK-1 inhibitor , PD 98059 , affected it to a lesser extent . - pubmed
These data demonstrate that SIN-1 induces COX-2 in HUVEC in the absence of PG formation and suggest a complex regulation of COX-2 expression and PG formation by NO in endothelial cells . - pubmed
To block the Fas - Fas ligand interaction , an anti - Fas ligand antibody ( FasL ) was injected with ADR 7 , 8 , and 9 wk after first administration of ADR . - pubmed
Fas antigen was overexpressed in the hearts of ADR and ADR+FasL groups , however , an expression of Fas antigen in the pirarubicin group was similar to the expression of Fas antigen in the control group . - pubmed
Synthesis of calcineurin - resistant derivatives of FK506 and selection of compensatory receptors . - pubmed
We used olefin metathesis to synthesize C40 derivatives of FK506 and measured their ability , when complexed to FKBP12 , to inhibit calcineurin ' s phosphatase activity . - pubmed
Using chemical genetic selection , we identified compensatory mutant CABs that respond to a calcineurin - resistant FK506 derivative at concentrations well below the response threshold for CABs containing only wild - type calcineurin sequence . - pubmed
The cytotoxicity of 5 against a panel of tumor cell lines , including several with multidrug resistance , and its effect on tubulin polymerization were comparable to epothilone D ( 4 ) . - pubmed
RESULTS : NOS 3 immunoreactivity and amount of protein ( western blotting ) were significantly decreased in CCl ( 4 ) cirrhotic livers . - pubmed
No changes were observed in systemic haemodynamics , in liver tests or urinary nitrates , or in NOS 3 expression in lung or kidney , indicating a highly selective transfer . - pubmed
These data indicate a major haemodynamic role of intrahepatic NOS 3 in the pathogenesis of portal hypertension in CCl ( 4 ) cirrhosis . - pubmed
The prevalence of mutations was lower among those receiving other nucleoside reverse transcriptase inhibitors and protease inhibitors . - pubmed
delivery of low doses of 8 - bromo - cGMP ( 0.1 - 0.25 micromol ) reduced nociceptive behavior and formalin - induced upregulation of PKG - I in the spinal cord . - pubmed
To explain these dose - dependent contrary effects we assessed the potential involvement of various cGMP targets : protein kinase G , cyclic nucleotide gated cation channels ( CNGs ) , phosphodiesterases ( PDE2 and PDE3 ) and AMPA - receptors . - pubmed
The PKG inhibitor , Rp-8 - bromo - cGMPS did not antagonize the antinociceptive effects of 8 - bromo - cGMP but caused antinociception itself . - pubmed
Inhibitors of CNGs , PDE2 and PDE3 had no effect on formalin evoked nociceptive behavior . - pubmed
Since AMPA receptor currents were found to be reduced by 8 - bromo - cGMP in vitro a direct or indirect reduction of AMPA receptor currents might possibly contribute to the antinociceptive effects of 8 - bromo - cGMP . - pubmed
On the other hand , 8 - bromo - cGMP evoked antinociception appears to be largely independent of PKG - I , CNGs , PDE2 and PDE3 . - pubmed
The antinociceptive effects of the PKG inhibitor suggest that a strong PKG activation may be responsible for ' high dose ' 8 - bromo - cGMP evoked hyperalgesia . - pubmed
BACKGROUND AND AIM OF THE STUDY : Calcification of bioprosthetic heart valves fabricated from glutaraldehyde ( GA)-pretreated heterograft tissue is frequently responsible for the clinical failure of these devices . - pubmed
Ethanol pretreatment of bioprosthetic heart valves was shown to inhibit cuspal calcification due to multiple mechanisms , including alterations of collagen structure and lipid extraction . - pubmed
AlCl3 pretreatment of bioprostheses to prevent calcification was also investigated ; this alters elastin structure , inhibits alkaline phosphatase , and complexes with phosphoesters , thereby inhibiting aortic wall mineralization . - pubmed
All tested compounds but one ( Ro 15 - 1570 ) revealed a dose - dependent inhibition of RNase P activity , indicating that they may have a direct effect on tRNA biogenesis . - pubmed
Activin A , hypoxanthine ( Hx ) , xanthine ( Xa ) plasma levels and absolute nucleated red blood cell ( NRBC ) count were measured . - pubmed
PHOSVITIN , THE IRON CARRIER OF EGG YOLK . - pubmed
Estrogen , another crucial factor in breast cancer , stimulates VEGF ; and an ERE in the VEGF gene has been defined . - pubmed
We show , using intratumoral microdialysis in vivo , that E(2 ) treatment increased tumor extracellular levels of VEGF in an estrogen - dependent breast cancer model . - pubmed
Arp residues expected to interact with nucleotide and with the first and second actin subunits in a daughter filament are conserved and similar to actin . - pubmed
This provides strong evidence that c - kit receptor mediated tyrosine kinase involvement in the pathogenesis of phyllodes tumors , and the therapeutic agent , STI571 , Glivec , may be a potentially useful drug for its management . - pubmed
PURPOSE : We report penile length ( PL ) responses to testosterone enanthate ( TE ) therapy for micropenis , and the relevance of the V89L polymorphism of SRD5A2 encoding the 5alpha - reductase type 2 and CAG repeat length polymorphism of AR encoding the androgen receptor . - pubmed
This method is tested on the F ( 2P )+ H2O-->HF+OH ( 2Pi ) reaction , which otherwise proves challenging to describe with traditional SA - MCSCF methods due to ( i ) different asymptotic degeneracies of reactant ( threefold ) and product ( twofold ) channels , and ( ii ) presence of low - lying charge transfer configurations near the transition state region . - pubmed
In this study , the effects of SJW on a benzodiazepine hypnotic , quazepam , which is metabolized by CYP3A4 , were examined . - pubmed
The urinary ratio of 6beta - hydroxycortisol to cortisol , which reflects CYP3A4 activity , also increased after dosing with SJW ( ratio ; 2.1 ( 95%CI 0.85 - 3.4 ) ) . - pubmed
CONCLUSIONS : These results suggest that SJW decreases plasma quazepam concentrations , probably by enhancing CYP3A4 activity , but does not influence the pharmacodynamic effects of the drug . - pubmed
BACKGROUND AND OBJECTIVES : Arginine 315 in factor VII ( FVII ) belongs to a solvent - exposed loop involved in direct interaction with the co - factor ( tissue factor , TF ) , in transmission of TF - induced effects and potentially in FVIIa inactivation . - pubmed
DESIGN AND METHODS : We characterized a mild coagulation FVII deficiency associated with reduced FVII activity ( 26 % ) and antigen ( 67 % ) . - pubmed
For PWWF / ADWF ratios in the upper range ( f(q ) approximately 2.0 ) , aerobic mass fractions in the lower range ( f(maer ) & lt ; 0.60 ) , and high ( usually raw ) wastewater strengths , the indicated mode of operation of MBR BNR systems is as extended aeration WWTPs . - pubmed
MMP-9 levels positively correlated with TIMP-1 and lactate dehydrogenase levels , and negatively with pH and glucose levels in pleural effusions . - pubmed
Short - term progestin treatments prevent estrous induction by a GnRH agonist implant in anestrous bitches . - pubmed
Abstract Raloxifene and low - dose simvastatin can each reduce low - density lipoprotein ( LDL ) cholesterol without affecting high - density lipoprotein ( HDL ) cholesterol and triglycerides . - pubmed
The objective of this double - blind , 12 - week study is to determine whether raloxifene and simvastatin coadministration gives added benefit beyond either monotherapy in affecting fasting lipoproteins and apolipoproteins . - pubmed
Each active treatment decreased non - HDL cholesterol compared with placebo ( P & lt ; .01 ) . - pubmed
Coadministration treatment was more effective than either monotherapy in reducing LDL cholesterol ( P & lt ; .05 ) . - pubmed
Coadministration treatment reduced mean apolipoprotein B ( P & lt ; .001 ) and non - HDL cholesterol ( P & lt ; .001 ) when compared with raloxifene , but was not significantly different when compared with simvastatin . - pubmed
Coadministration therapy increased HDL cholesterol and apolipoprotein A1 levels compared with placebo ( P & lt ; .02 ) . - pubmed
No significant effect on triglycerides , very low density lipoprotein cholesterol , and lipoprotein ( a ) occurred with any active treatment . - pubmed
Combining lapatinib ( GW572016 ) , a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases , with therapeutic anti - ErbB2 antibodies enhances apoptosis of ErbB2 - overexpressing breast cancer cells . - pubmed
Treating ErbB2 - overexpressing breast cancer cell lines with a relatively low concentration of lapatinib alone resulted in a minimal increase in tumor cell apoptosis with an associated decrease in steady - state protein levels of p - ErbB2 , p - Akt , p - Erk1 / 2 , and notably survivin , compared to baseline . - pubmed
Treatment with trastuzumab alone had relatively little effect on survivin and apoptosis was unaffected . - pubmed
Combining lapatinib with either pAb or trastuzumab markedly downregulated survivin protein and enhanced tumor cell apoptosis . - pubmed
Ifosfamide must be activated by cytochrome P450 ( P450 ) enzymes in the liver , initially to a short lived intermediate and then to toxic metabolites that are subsequently distributed by the circulatory system . - pubmed
In this study , we examined the feasibility of fibrin glue , mixed with hyaluronic acid ( HA ) as a composite scaffold . - pubmed
These results indicate that the metabolism of iprodione involves Phase II biotransformation in the liver ( XRE , GSTYa ) , and that this chemical has the potential to cause cell proliferation and/or inflammatory reactions ( c - fos , NF - kB ) , proteotoxic effects ( HSP70 , GRP78 ) , metabolic disruption ( CRE ) , and DNA damage ( GADD45 , GADD153 ) . - pubmed
However , atrazine metabolites , deethylatrazine ( DEA ) and deisopropylatrazine ( DIA ) , were detected in plant roots and the AM colonization enhanced the metabolism . - pubmed
The only drugs currently approved for the treatment of HBV infection are lamivudine , adefovir , entecavir , and interferon - a. - pubmed
After achieving steady - state exercise , L - NAME ( 25 mg ) was infused over 5 min to inhibit NO synthase . - pubmed
Dramatic clinical efficacy of cladribine in Rosai - Dorfman disease and evolution of the cytokine profile : towards a new therapeutic approach . - pubmed
Our findings show the promising efficacy of cladribine and suggest that therapies targeting specifically cytokines might be useful in some cases of active RDD . - pubmed
This was a predetermined secondary end point of a randomized clinical trial designed to assess progression of coronary artery calcium ( CAC ) scores in the two treatment arms . - pubmed
Mortality was borderline significantly lower in subjects randomized to sevelamer ( 5.3/100 patient years , confidence interval ( CI ) ( 2.2 - 8.5 ) compared to those randomized to calcium - containing binders ( 10.6/100 patient years , CI 6.3 - 14.9 ) ( P=0.05 ) . - pubmed
The association of systolic blood pressure ( SBP ) with the MMSE score through the follow - up period was analysed controlling for frailty ( time to death ) , age , gender , apoprotein E , homocysteine , hypertension , congestive heart failure , and stroke . - pubmed
These findings suggest the presence of the specific vitamin C transporter SVCT in retina and RPE , which may be involved in the manifestation of diabetic retinopathy . - pubmed
Using multivariate studies , four major CPE variables were evaluated : Triton X-114 concentration , sample volume , NaCl concentration , and pH. - pubmed
The results show that surfactant concentration and sample volume were the main variable affecting the CPE process , with the following optimized parameters : 1 % ( w / v ) Triton X-114 concentration , 50 microL of sample volume , 6 % ( w / v ) NaCl concentration and extractions carried out at pH 7.0 . - pubmed
Structural basis for the activity of the RSK - specific inhibitor , SL0101 . - pubmed
SL0101 is a potent and specific inhibitor of RSK ; therefore , we performed an analysis of the structural basis for the inhibitory activity of this lead compound . - pubmed
In in vitro kinase assays we found that acylation of the rhamnose moiety and the 4 ' , 5 , and 7 - hydroxyl groups are responsible for maintaining a high affinity interaction of RSK with SL0101 . - pubmed
It is likely that the hydroxyl groups facilitate RSK binding through their ability to form hydrogen bonds . - pubmed
We found that acylation of the rhamnose moiety was essential for maintaining the selectivity for RSK inhibition in intact cells . - pubmed
Further , the efficacy of SL0101 in intact cells is limited by cellular uptake as well as possible hydrolysis of the acetyl groups on the rhamnose moiety by ubiquitous intracellular esterases . - pubmed
These studies should facilitate the development of a RSK inhibitor , based on the SL0101 pharmacophore , as an anti - cancer chemotherapeutic agent . - pubmed
As rejection rates were higher in the absence of CsA , further study of calcineurin inhibitor - free regimens is required before general recommendations can be made . - pubmed
In this syndrome , we believe that high levels of circulating epinephrine trigger a switch in intracellular signal trafficking in ventricular cardiomyocytes , from G ( s ) protein to G ( i ) protein signaling via the beta ( 2 )- adrenoceptor . - pubmed
Importantly , administration of olomoucine , a selective CDK inhibitor , not only suppressed astroglial proliferation and glial scar formation , but also decreased neuronal cell death in the ischemic boundary zone and hippocampal CA1 region at days 1 and 30 after MCAO . - pubmed
Retrospective detection of sulfur mustard exposure by mass spectrometric analysis of adducts to albumin and hemoglobin : an in vivo study . - pubmed
The albumin adduct ( S - HETE ) Cys - Pro - Tyr was detectable up to 7 days after the exposure , while the adduct to the N - terminal valine in hemoglobin was still detected after 28 days . - pubmed
Remarkably , the N - terminal valine adduct to hemoglobin increased during the first three days , which implies that there is still free sulfur mustard present during that time . - pubmed
OBJECTIVE : To compare efficacy of sublingual ( S / L ) , oral and vaginal routes of misoprostol administration for cervical priming before suction evacuation ( SE ) under local anaesthesia . - pubmed
Autologous PRGF activated with calcium chloride was used to infiltrate the injured tissues . - pubmed
Nine skilled players ( 21.0+/-2.9 yr ) underwent a graded maximal aerobic test ( GT ) where V O(2)max and HR - V O ( 2 ) relationship were determined . - pubmed
V O ( 2 ) , HR and blood lactate ( [ La](b ) ) were recorded during a 2 x 225 s ( interspersed with 30s rest ) 4 - a - side handball game and were compared to those measured during an 480 - s running intermittent exercise ( IE ) . - pubmed
The HR - V O ( 2 ) relationship during GT was high ( r(2)=0.96 , p<0.001 ) but estimated V O(2 ) from HR was lower to that measured ( p=0.03 ) in handball , whereas there was no difference in IE . - pubmed
The biochemical hypercortisolemia together with the prolactin and androgen levels were evaluated to explore the cause of her anovulation . - pubmed
Adrenal Cushing ' s syndrome was confirmed on the basis of the elevated urinary free cortisol ( 454 microg/24h , normal range : 20 - 70 ) with a suppressed ACTH level ( 2.0 pg / mL , normal range : 6.0 - 76.0 ) and the loss of circadian cortisol secretion . - pubmed
The behavior at the air / water interface and the structures of Langmuir - Blodgett monolayers at different surface pressures of rod - coil molecules , which consist of a Y - shaped rigid aromatic segment containing peripheral tetradecyloxy groups and a flexible poly ( ethylene oxide ) ( PEO ) chain with 17 , 21 , 34 , or 45 repeating ethylene oxide units ( Y17 , Y21 , Y34 , and Y45 ) , were investigated . - pubmed
Expressed prostatic secretions ( EPS ) were obtained to determine the leukocyte count , PMN elastase ( ELISA ) and citric acid concentrations ( UV method ) , and the occurrence of C. - pubmed
The AlkB family proteins utilize iron ( II ) , alpha - ketoglutarate ( alpha - KG ) and dioxygen to perform oxidative repair of alkylated nucleobases in DNA and RNA . - pubmed
In addition , these two proteins can catalyze the demethylation of 3 - meU in ssRNA with a slightly higher efficiency over that of 3 - meT in ssDNA , suggesting that methylated RNAs are the preferred substrates for FTO . - pubmed
Nonenzymatic role of acetylcholinesterase in neuritic sprouting : regional changes in acetylcholinesterase and choline acetyltransferase after neonatal 6 - hydroxydopamine lesions . - pubmed
Parallel examinations of choline acetyltransferase , an enzymatic marker for cholinergic nerve terminals , showed a different pattern of 6 - OHDA - induced alterations , with initial decreases in both forebrain and brainstem in males and regression toward normal by PN21 ; females were far less affected . - pubmed
Further , the results for choline acetyltransferase indicate that early depletion of norepinephrine compromises development of acetylcholine systems , consistent with a trophic role for this neurotransmitter . - pubmed
Activated carbon cloth ( ACC ) was used as adsorbent for the removal of cationic surfactants such as benzyltrimethylammonium chloride ( BTMACl ) , benzyltriethylammonium chloride ( BTEACl ) , benzyltributylammonium chloride ( BTBACl ) , benzyldimethyldecylammonium chloride ( BDMDACl ) , benzyldimethyltetradecyl ammonium chloride ( BDMTDACl ) , benzyldimethylhexadecylammonium chloride ( BDMHDACl ) , N - dodecylpyridinium chloride ( N - DPCl ) and N - cetylpyridinium chloride ( CPCl ) from aqueous solutions . - pubmed
Ion specific effects of sodium and potassium on the catalytic activity of HIV-1 protease . - pubmed
The behavior of HIV-1 protease in aqueous NaCl and KCl solutions is investigated by kinetic measurements and molecular dynamics simulations . - pubmed
Sodium is attracted more strongly than potassium to the protein surface primarily due to stronger interactions with carboxylate side chain groups of aspartates and glutamates . - pubmed
These effects are of particular importance for acidic amino acid residues at or near the active site of the enzyme , including a pair of aspartates at the entrance to the reaction cavity . - pubmed
RNF122 : a novel ubiquitin ligase associated with calcium - modulating cyclophilin ligand . - pubmed
From a yeast two - hybrid screen , we identified calcium - modulating cyclophilin ligand ( CAML ) as an RNF122 - interacting protein . - pubmed
The CYP21A2 H365Y mutant exhibited minimal 21 - hydroxylase activity to convert 17 - hydroxyprogesterone to 11 - deoxycortisol or progesterone to 11 - deoxycorticosterone . - pubmed
Threonine ( T ) was also substituted with alanine ( A ) at position 8 ( T1087A ) more frequently in A*03 - positive patients ( 6/12 ) compared with controls ( 2/33 , p=0.01 ) , and the double substitution of TK to AR was also observed predominantly in HLA - A * 03 - positive patients ( p=0.004 ) . - pubmed
After extensive genetic and metabolic investigation , a nucleotide substitution c.2207 A G in the POLG gene resulting in amino acid change Asn 736Ser in exon 13 was demonstrated . - pubmed
Antioxidant defenses consisting of contents of reduced glutathione ( GSH ) and glutathione disulfide ( GSSG ) and activities of superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione reductase ( GR ) , selenium - dependent glutathione peroxidase ( Se - GPx ) and glutathione S - transferase ( GST ) in liver of freshwater fish were determined and the GSH - GSSG ratio and content of tGSH ( total glutathione ) were calculated . - pubmed
In addition , hepatic SOD and GSH , especially tGSH , were sensitive to 2,4 - DCP contamination and thus , can possibly be used in early assessment of 2,4 - DCP - dominant polluted aquatic ecosystems . - pubmed
Moreover , ALK5 inhibition using the SB431542 inhibitor significantly affected CTGF expression only at later time points whereas ERK1 / 2 inhibition completely abrogated CTGF expression . - pubmed
The Cellular Prion Protein Prevents Copper - Induced Inhibition of P2X ( 4 ) Receptors . - pubmed
Although the physiological function of the cellular prion protein ( PrP(C ) ) remains unknown , several evidences support the notion of its role in copper homeostasis . - pubmed
PrP ( C ) binds Cu ( 2 + ) through a domain composed by four to five repeats of eight amino acids . - pubmed
Previously , we have shown that the perfusion of this domain prevents and reverses the inhibition by Cu ( 2 + ) of the adenosine triphosphate ( ATP)-evoked currents in the P2X ( 4 ) receptor subtype , highlighting a modulatory role for PrP ( C ) in synaptic transmission through regulation of Cu ( 2 + ) levels . - pubmed
Here , we study the effect of full - length PrP(C ) in Cu ( 2 + ) inhibition of P2X ( 4 ) receptor when both are coexpressed . - pubmed
PrP ( C ) expression does not significantly change the ATP concentration - response curve in oocytes expressing P2X ( 4 ) receptors . - pubmed
However , the presence of PrP(C ) reduces the inhibition by Cu ( 2 + ) of the ATP - elicited currents in these oocytes , confirming our previous observations with the Cu ( 2 + ) binding domain . - pubmed
Thus , our observations suggest a role for PrP ( C ) in modulating synaptic activity through binding of extracellular Cu ( 2 + ) . - pubmed
We detected the activities of GPx , GRd and SOD in the liver , and the levels of inflammatory mediators including IL-1beta , IL-6 , TNF - alpha , COX-2 , MDA and NO in the edema paw to investigate the mechanism of action against inflammation . - pubmed
EO(MeOH ) increased the activities of SOD and GPx in liver tissue and decreased MDA , NO , IL-1beta , IL-6 , TNF - alpha and COX-2 levels in paw edema tissue at the 3rd h after lambda - carrageenan - induced inflammatory reaction . - pubmed
It was indicated that the anti - inflammatory mechanism of EO(MeOH ) may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD , GPx and GRd in the liver . - pubmed
Additionally , EO(MeOH ) decreased IL-1beta , IL-6 , TNF - alpha and COX-2 levels in the edema paw . - pubmed
EphA4 receptor ( EphA4 ) tyrosine kinase is an important regulator of central nervous system development and synaptic plasticity in the mature brain , but its relevance to the control of normal behavior remains largely unexplored . - pubmed
In addition , immunohistological analyses revealed that some of the neurons in the ectodermal epithelium are GLWamide- and PRGamide - positive . - pubmed
This inhibition is due to the binding by Grft of high - mannose saccharides on the surface of gp120 . - pubmed
AREAS COVERED : Today an array of biologics blocking either proinflammatory cytokines or lymphocyte activation / survival are available that enable a substantial improvement over conventional disease - modifying antirheumatic drugs ( DMARDs ) . - pubmed
Loss of function mutations and non - conservative amino acid replacements affected several habitat- and lifestyle - associated traits , for example , the key regulator GacS of the switch between acute and chronic disease phenotypes was disrupted in strain CHA . - pubmed
Comparison of Effectiveness of Betamethasone gel Applied to the Tracheal Tube and IV Dexamethasone on Postoperative Sore Throat : A Randomized Controlled Trial . - pubmed
This randomized controlled trial study investigated the incidence of postoperative sore throat after general anesthesia when betamethasone gel is applied to a tracheal tube compared with when IV dexamethasone is prescribed . - pubmed
RESULTS : The incidence of sore throat was significantly lower in the betamethasone gel group compared with the IV dexamethasone and control groups , 1 , 6 , and 24 hours after surgery . - pubmed
In the first day after surgery 10.7 % of the betamethasone group had sore throat whereas 26.7 % of the IV dexamethasone group and 30.7 % of the control group had sore throat . - pubmed
CONCLUSION : We concluded that wide spread application of betamethasone gel over tracheal tubes effectively mitigates postoperative sore throat , compared with IV dexamethasone application . - pubmed
Changes in follicle - stimulating hormone ( FSH ) , testosterone , sperm motility , semen volume , and sperm morphology were also examined . - pubmed
There were no significant differences between placebo and pregabalin groups with respect to their effects on FSH , testosterone , or sperm motility . - pubmed
However , in the presence of TALE factors , RNAPII remains phosphorylated on serine 5 and hoxb1a transcription is inefficient . - pubmed
This triggers P - TEFb - mediated transitioning of RNAPII to the serine 2 - phosphorylated form and efficient hoxb1a transcription . - pubmed
RESULTS : ECLS significantly improved renal ( creatinine 1.86 + - 0.91 vs 1.32 + - 0.52 mg / dl , P = 0.02 ) , hepatic ( aspartate aminotransferase 1426 + - 2176 vs 277 + - 259 U / l , P = 0.04 ; alanine aminotransferase 982 + - 1466 vs 357 + - 447 U / l , P = 0.04 ) and pulmonary functions ( fraction of inspired oxygen 52 + - 18 vs 26 + - 23 % , P & lt ; 0.01 ; positive end - expiratory pressure 7 + - 3 vs 5 + - 4 mbar , P = 0.02 ) over a period of 8 + - 7 days . - pubmed
The structure of this new polyglucuronic acid sodium salt was analyzed by ( 13 ) C NMR spectra and HPAEC - PAD chromatography . - pubmed
Cyclophosphamide and IL-12 - transduced DCs enhance the antitumor activity of tumor antigen - stimulated DCs and reduce Tregs and MDSCs number . - pubmed
The highest level of tumor growth inhibition , accompanied by high cytotoxic activity of effector cells , and their increased influx into tumor tissue , was observed after application of cyclophosphamide in combination with BMDC / TAg and BMDC / IL-12 . - pubmed
AIM : To assess the effects and safety of iron - based phosphate binders in adult patients receiving dialysis . - pubmed
Anthropometric parameters , serum IL-6 , IL-18 , CRP , glucose , insulin concentrations and insulin sensitivity / resistance indexes were evaluated . - pubmed
Moreover , we confirmed specific electrophysiological responses from different neuronal units in the thalamus , in response to particular types of painful stimuli ( i , e. , formalin injection , pinch ) , which we found to be modulated by optogenetic control of the ACC inhibitory neurons . - pubmed
Recently , a dual - target indol-3ylglyoxyldipeptide derivative , designed to bind to the Translocator Protein ( TSPO ) and reactivate p53 function via dissociation from its physiological inhibitor , murine double minute 2 ( MDM2 ) , has been developed as a potent GBM pro - apoptotic agent . - pubmed
Changes in the glucose avidity of mediastinal nodal metastases may be a stronger predictor of survival than changes in the primary site of disease . - pubmed
Phosphatase 2A ( PP2A ) is an important serine and threonine phosphatase . - pubmed
Prospects of medium specific activity ( 177 ) Lu in targeted therapy of prostate cancer using ( 177 ) Lu - labeled PSMA inhibitor . - pubmed
Targeted radionuclide therapy using ( 177 ) Lu - labeled peptidomimetic inhibitor of prostate specific membrane antigen ( PSMA ) viz . - pubmed
A stereoselective total synthesis of ( + ) - marrubiin has been accomplished starting from a chiral building block via the CyNH2 - promoted Pauson - Khand reaction ( PKR ) followed by oxidative cleavage of the resultant cyclopentenone ring . - pubmed
OBJECTIVE : To evaluate the feasibility and image quality of low - dose CT of the paranasal sinuses using iterative reconstruction with adaptive - iterative dose reduction in three dimensions ( AIDR 3D ) in comparison with conventional image protocols of older scanner generations . - pubmed
Cross - sectional TEM investigation reveals that the utilization of the diamond interlayer circumvented the formation of amorphous boron nitride prior to the growth of hexagonal boron nitride . - pubmed
Glutamate metabolism was indicated by expression of glutamine synthase ( GS ) , glutamate - aspartate transporter ( GLAST ) and glutamate transporter-1 ( GLT-1 ) at both protein and gene levels . - pubmed
Compared to the control group , exposure to 2 - CE could cause a dose dependent damage in astrocytes , indicated by decreased cell viability and morphological changes , and supported by decreased levels of nonprotein sulfhydryl ( NPSH ) and inhibited activities of Na +/ K +- ATPase and Ca2 + - ATPase in the cells . - pubmed
A first intravenous dose of bisphosphonates may be associated with an acute - phase response ( APR ) . - pubmed
Prior bisphosphonate exposure was predictive of APR risk and severity . - pubmed
INTRODUCTION : Intravenous ( IV ) administration of bisphosphonates ( BP ) , such as zoledronate ( ZOL ) and ibandronate ( IBN ) , may be associated with an APR . - pubmed
Prior oral or IV BP use was less frequent in the ZOL group ( 61.8 vs. 71.7 % , p = 0.005 ) . - pubmed
Prior BP exposure was predictive of both APR risk and severity , and lower serum 25 - hydroxy vitamin D ( 25 ( OH ) D ) levels were possibly predictive of severity . - pubmed
CONCLUSIONS : In a real - world setting , APR rates with ZOL and IBN may be higher than reported in randomised controlled trials and may differ by compound , prior BP exposure , and serum 25 ( OH ) D levels . - pubmed
Staining techniques most commonly used included the following : cytokeratin 7 ( 94 % ) , KIT ( 71 % ) , vimentin ( 65 % ) , colloidal iron ( 59 % ) , CD10 ( 53 % ) , and AMACR ( 41 % ) . - pubmed
The results suggest that signaling proteins affected by EGCG in ovarian cancer , which include JUN , FADD , NFKB1 , Bcl-2 , HIF1alpha , and MMP , are involved primarily in cell cycle , cellular assembly and organization , DNA replication , etc . - pubmed
Extended stability of the rituximab biosimilar CT - P10 in its opened vials and after dilution and storage in polyolefin bags . - pubmed
The stability of the rituximab biosimilar CT - P10 , in 50mL vials at a concentration of 10mg / mL , and after dilution to final concentrations of 1 and 4mg / mL and storage in polyolefin bags at 4 C and 25 C was studied by several orthogonal and complementary methods . - pubmed
Lung glutathione was significantly depleted across all CS groups , however the FD group had the most severe , ongoing oxidative stress . - pubmed
Notably , the HgP per unit of TSS at the burned site was an order of magnitude higher than the unburned site ( 2.66 and 0.13 ng HgP per mg TSS , respectively ) for 8 months following the fire , resulting in elevated HgP concentrations for the range of flow conditions , after which there was a rapid return to non - disturbed conditions . - pubmed
We developed new photoresponsive polydiacetylene ( PR - PDA ) molecules by incorporating a photocleavable moiety , 6 - nitropiperonyl alcohol ( NP ) or 4,5 - dimethoxy-2 - nitrobenzyl alcohol ( DMN ) , into a self - assembling diacetylene molecule . - pubmed
In this paper , a WSe2 film prepared by chemical vapor deposition ( CVD ) is transferred onto a tapered fiber , and a WSe2 saturable absorber ( SA ) is fabricated . - pubmed
The modulation depth of the WSe2 SA is measured as being 21.89 % . - pubmed
Taking advantage of the remarkable nonlinear absorption characteristic of the WSe2 SA , a mode - locked erbium - doped fiber laser is demonstrated at 1557.4 nm with a bandwidth of 25.8 nm and signal to noise ratio of 96 dB. - pubmed
Interleukin-6 or tumor necrosis factor - alpha blockade treatments were not effective , the oral administration of colchicine was stared under the immunosuppressive treatments with steroid and cyclosporine A ( CyA ) . - pubmed
The dose of prednisolone was successfully tapered , and the elevated levels of C - reactive protein were normalized . - pubmed
Moreover , VU0155041 treatment substantially blocked SHH - induced cyclin D1 expression and cell proliferation , while increasing TUNEL - positive cells and the activation of apoptosis - related proteins . - pubmed
We could estimate whether a vessel was an artery or a vein by using the S - factor obtained from the PA images at two wavelengths , which corresponds approximately to the haemoglobin oxygen saturation . - pubmed
A779 attenuated captopril - mediated improvements in insulin sensitivity ( 23 + - 2 mg kg-1 min-1 ; P = 0.042 ) , with no effect on body weight ( 32 + - 2 g ; P = 0.441 ) or BP ( 111 + - 7 mmHg ; P = 0.788 ) . - pubmed
Tanshinone IIA protects lens epithelial cells from H2 O 2 -induced injury by upregulation of lncRNA ANRIL . - pubmed
In the meantime , the expression of lncRNA ANRIL was upregulated by Tanshinone IIA . - pubmed
Moreover , the elevated expression of lncRNA ANRIL induced by Tanshinone IIA was abolished by BAY 11 - 7082 ( an inhibitor of NF - kappaB ) . - pubmed
Tanshinone IIA confers its protective effects possibly via modulation of NF - kappaB signaling and thereby elevating the expression of lncRNA ANRIL . - pubmed
Dihydrotestosterone inhibits arylsulfatase B and Dickkopf Wnt signaling pathway inhibitor ( DKK )- 3 leading to enhanced Wnt signaling in prostate epithelium in response to stromal Wnt3A. - pubmed
The effects of androgen exposure on ARSB and the impact of decline in ARSB on Wnt signaling in prostate tissue were unknown . - pubmed
The effects of DHT and of ARSB overexpression on DKK expression were determined . - pubmed
Exposure to 5alpha - dihydrotestosterone ( DHT ) increased ARSB and DKK3 promoter rmethylation , and effects of DHT on DKK3 expression were reversed when ARSB was overexpressed . - pubmed
CONCLUSIONS : Androgen - induced declines in ARSB and DKK3 may contribute to prostate carcinogenesis by sustained activation of Wnt signaling in prostate epithelium in response to stromal Wnt3A. - pubmed
In vitro , PTXL showed better ability on inhibiting cell proliferation , migration and invasion , and effectively on promoting apoptosis and arresting cell cycle in G2 / M phase compared to PTX . - pubmed
Finally , we found PTX treatment suppressed the production of tumor necrosis factor alpah ( TNF - alpha ) , interleukin 6 ( IL-6 ) and transforming growth factor beta ( TGF - beta ) and inhibited the expression of alpha smooth muscle actin ( alpha - SMA ) and collagen I in HKFs . - pubmed
The activation of protein kinase B ( AKT)/glycogen synthase kinase 3 beta ( GSK3beta ) signaling pathway also blocked by PTX in cultured HKFs and keloid tissues . - pubmed
LY294002 , a PI3 K ( phosphatidylinositol 3 - kinase)/AKT inhibitor , also suppressed the expression of TNF - alpha , IL-6 and TGF - beta , and simultaneously , reduced the production of alpha - SMA and collagen I in HKFs . - pubmed
The inhibition of AKT / GSK3beta signaling pathway contribute to inhibit the generation of fibrogenic cytokines by PTXL on ameliorating fibrosis progress in keloids . - pubmed
Mean serum FGF23 concentration and standard deviation of participants at baseline and after vitamin D3 supplementation were extracted to calculate standard mean difference ( SMD ) . - pubmed
The meta - analyses found that serum intact FGF23 increased significantly after oral vitamin D3 supplementation in vitamin D - deficient participants with the pooled SMD of 0.36 ( 95%CI , 0.14 , 0.57 ; p = 0.001 ; I2 of 36 % ) . - pubmed
Serum C - terminal FGF23 also increased after vitamin D3 supplementation in vitamin D - deficient participants with the pooled SMD of 0.28 although without reaching statistical significance ( 95%CI , - 0.08 , 0.65 ; p = 0.13 ; I2 of 0 % ) . - pubmed
The present systematic review and meta - analysis revealed that serum intact FGF23 concentration increased significantly after oral vitamin D3 supplementation in vitamin D - deficient participants . - pubmed
After 1h , hepatic and splenic tissue samples were harvested for TBARS , CAT , GPx and GSH - Rd measurement . - pubmed
Furthermore , the hepatic and splenic activities of CAT , GPx and GSH- Rd were significantly higher in sIPC group than in IRI group . - pubmed
BACKGROUND : A confounding issue in [ 18F ]- NaF PET / CT imaging of abdominal aortic aneurysms ( AAA ) is the spill in contamination from the bone into the aneurysm . - pubmed
CONCLUSION : The background correction ( OSEM + PSF + BC ) technique provided more robust AAA quantitative assessments regardless of the AAA ROI delineation method , and thus it can be considered as an effective spill in correction method for [ 18F ]- NaF AAA studies . - pubmed
The extract was able to reduce total cholesterol and non - high - density lipoprotein cholesterol ( C - HDL ) levels and increase the C - HDL levels reduced in diabetes , decreasing the atherogenic index and therefore the risk of suffering CVD at the same level as atorvastatin . - pubmed
HRM was also used for the detection of adulteration of olive oil with oils of different plant origin by using plastid trnL indels and SNPs . - pubmed
The trnL - indels - HRM showed higher discrimination power than the trnL - SNP - HRM in determining adulteration in olive oil . - pubmed
Meanwhile , it remained a rejection of 95.85 % bovine serum albumin ( BSA ) , which was also better than that of the pristine PES membrane . - pubmed
The enzyme showed a great deal of stability in the presence of ethanol , methanol , acetone , 2 - propanol , dimethyl sulfoxide , Tween-80 and Triton X-100 . - pubmed
A series of collagen fibrils with diameters of 26 - 230 nm were harvested using this process , and in situ observations under polarizing microscopy ( POM ) and using molecular dynamics simulations revealed the influence of the NaOH / urea system on the tendon collagen . - pubmed
Vesicles prepared by exposure of spheroplasts to ultrasound contained cytochromes a , b and c , and at 50 degrees C they rapidly oxidized NADH and ascorbate in the presence of tetramethyl - p - phenylenediamine . - pubmed
Hydrolysis of ATP by a dicyclohexylcarbodi - imide - sensitive adenosine triphosphatase was accompanied by a similarly directed net translocation of H (+ ) . - pubmed
The last result suggested an energy - conserving site in the respiratory chain between cytochrome c and oxygen . - pubmed
Under anaerobic conditions the reduction of cytochrome b by ascorbate ( with tetramethyl - p - phenylenediamine ) was stimulated by ATP hydrolysis , indicating an energy - conserving site between cytochrome b and cytochrome c. - pubmed
However , no reduction of NAD (+ ) supported by oxidation of succinate , malate or ascorbate occurred , neither did it with these substrates in the presence of ATP under anaerobic conditions , suggesting that there was no energy - conserving site between NADH and cytochrome b. - pubmed
These effects were not observed when phenazine methosulphate , which transfers electrons from succinate dehydrogenase directly to oxygen , was present . - pubmed
It was concluded that in these vesicles the oxidation of succinate was energy - dependent and that the reoxidation of reduced succinate dehydrogenase was dependent on the outward movement of H (+ ) by the protonmotive force . - pubmed
This may be related to the need for an ample supply of succinate for haem synthesis in order to provide cytochromes for the organism . - pubmed
A mutant of MP1 , MP5 , had increased susceptibility to amikacin and reduced phosphotransferase activity . - pubmed
MP9 , a mutant MP5 , was more resistant to amikacin and had increased phosphotransferase activity . - pubmed
The mutations leading to these alterations of amikacin susceptibility and amikacin phosphotransferase activity were transferable with the same plasmid that was associated with amikacin resistance and phosphotransferase activity in MP1 . - pubmed
[ Scope and limitation of the analytical use of dansyl chloride , II : Formation of secondary dansyl amines by the reaction of dansyl chloride with N - oxides of aliphatic tertiary amino derivatives according to the Polonovski reaction ( author 's transl ) ] . - pubmed
In contrast , carbohydrate - induced preservation of labile enzymes during freeze - drying is a fundamentally different process . - pubmed
Using Fourier transform intra - red spectroscopy we have found that hydrogen bonding between the carbohydrate and the dried protein is required for stabilization . - pubmed
Thus , it appears that certain carbohydrates protect dried enzymes because these solutes serve as water substitutes for the dried protein , by satisfying the hydrogen bonding requirement of polar groups on the protein 's surface . - pubmed
From the time course of the release of prosaposin induced by thrombin and from the fact that weak platelet agonists , ADP , epinephrine , and collagen , did not cause the release of prosaposin , prosaposin secretion from platelets seemed to be from lysosome like granules . - pubmed
Reduction of Cl- in tubular fluid from 84 to 8 mM also decreased Acl from 16.1 to 9.9 mM and hyperpolarized Vbl by 12 mV. - pubmed
Because a previous study suggested that electroneutral NaCl reabsorption is mediated by Na +/ H (+)- Cl -/ HCO3 - exchangers in the apical membrane , the Cl-/HCO3- exchange inhibitor , 4,4 - diisothiocyanostilbene-2 - 2 - disulphonic acid ( DIDS ) was added to tubule fluid . - pubmed
Total protein and albumin are significantly less in neonatal serum , whereas bilirubin ( total and direct ) is significantly higher in neonatal serum than in adult serum ( P less than 0.01 ) . - pubmed
The parameters studied included blood serotonin levels , monoamine oxidase ( MAO ) activity in the blood serum , as well as urinary excretion of adrenaline , noradrenaline , dopamine , DOPA and 5 - HOIAA . - pubmed
Following a course of AP they presented a compensatory increase in MAO activity , normalization of blood serotonin levels and of the urinary excretion of noradrenaline , dopamine and DOPA . - pubmed
Before treatment , serum free T4 correlated significantly , either positively or negatively , with the basal metabolic rate ( BMR ) , duration of the achilles tendon reflex ( ATR ) , resting pulse rate ( RPR ) , serum total cholesterol ( T - cholesterol ) , ratio of the pre - ejection period to the left ventricular ejection time ( PEP / LVET ) , serum high density lipoprotein ( HDL ) and creatine phosphokinase ( CPK ) in the descending order . - pubmed
A series of 64 punch biopsies collected from women prospectively followed - up for cervical Human papillomavirus ( HPV ) infections ( with and without CIN ) , and 38 control biopsies ( normal epithelia , and classical CIN ) were analysed for expression of filaggrin ( a histidine - rich protein constituent of keratohyalin granules ) using the ABC technique and polyclonal antibody . - pubmed
Reversal of scopolamine - induced amnesia of passive avoidance by pre- and post - training naloxone . - pubmed
Post - training administration of naloxone ( 3 mg / kg ) as a single or as a split dose also attenuated the scopolamine - induced amnesia . - pubmed
Control experiments indicated that neither an increase in pain sensitivity ( pre - training naloxone ) nor an induced aversive state ( post - training naloxone ) appear to be responsible for the influence of naloxone on the scopolamine - induced retention deficit . - pubmed
Although the peroxidative metabolism of benzidine and other carcinogenic arylamines has been regarded as a significant pathway in extrahepatic tissues , the mechanism of peroxidase - mediated covalent binding to DNA has remained unclear . - pubmed
In this study , we have compared the metabolic activation of benzidine by prostaglandin H synthase , horseradish peroxidase , chloroperoxidase , and lactoperoxidase . - pubmed
All four peroxidases mediated the binding of benzidine to DNA and equimolar amounts of hydrogen peroxide ( or arachidonic acid ) and benzidine were required for the maximal binding in the system with either horseradish peroxidase or prostaglandin H synthase . - pubmed
Two major and one minor deoxyguanosine adducts ( P - I , P - III and P - IV , respectively ) were isolated after the enzymatic hydrolysis of the benzidine - modified DNA . - pubmed
The interrelationships of alcohol dehydrogenase and the aldehyde dehydrogenases in the metabolism of ethanol in liver . - pubmed
In 10 hemodialyses ( HD ) with cuprophan ( CU ) and 10 with polyacrylonitrile ( PAN ) , signs of complement activation were investigated by following arterial and venous levels of C3a , C3d and C5a , in order to propose a marker of bioincompatibility . - pubmed
HD using PAN membranes were associated with a lower C3a , C3d and C5a generation and fewer dialyses generating these products . - pubmed
The concentrations of glycerol and phosphatidylglycerol in amniotic fluid were measured by using a coupled enzymatic assay with and without phospholipase D ( EC 3.1.4.4 ) . - pubmed
( 3 ) Exercise - induced increases in O2 uptake and blood lactate concentration were both attenuated by LBNP and exaggerated by LPP . - pubmed
The present study was designed to determine whether the central sympatholytic effects of ketanserin result from the ability of the drug to block serotonin2 or alpha-1 adrenergic receptors . - pubmed
These data , along with earlier results from our laboratory , provide strong evidence to indicate that ketanserin produces a centrally mediated inhibition of sympathetic nerve discharge as a result of the ability of the drug to block alpha-1 adrenergic receptors . - pubmed
Actual and absolute deviations from the reference standard demonstrate that the Accuchek bG and Glucoscan 2000 monitors provide relatively unbiased estimates of blood glucose , whereas the Glucokey , Glucochek II , Glucometer II , and Trendsmeter generally underestimate the true values . - pubmed
Synthesis of cytochromes and heme by mutant H-4 was dependent upon delta - aminolevulinate ; this strain did not form bacteriochlorophyll either with or without delta - aminolevulinate and , consequently , grew only under aerobic conditions . - pubmed
The mutants differed in their ability to take up radioactive delta - aminolevulinate from the external environment ; mutant H-5 was less active than mutant H-4 or the wild type . - pubmed
spheroides made only one form of delta - aminolevulinate synthase , which provided delta - aminolevulinate for bacteriochlorophyll and heme synthesis . - pubmed
A rapid microassay of lysozyme activity with reduced chitin oligosaccharides as substrate . - pubmed
As the serum digoxin level reached 21.7 ng / ml digoxin - specific Fab - antibody - fragments were used to bind free serum digoxin . - pubmed
pneumophila phagosome and monocyte lysosomes were investigated by prelabeling the lysosomes with thorium dioxide , an electron - opaque colloidal marker , and by acid phosphatase cytochemistry . - pubmed
USA 78 , 2717 - 2721 , 1981 ) , we conclude that the tryptophan codon UGG in position 234 has been changed into a UGA nonsense codon in the CyOnB mutant . - pubmed
The analyses also demonstrate that the importance of Na + entry across the basolateral membrane is strongly dependent upon transepithelial potential , cmNa and csK ; under certain conditions , net Na + entry could be appreciably greater across the basolateral than across the apical membrane . - pubmed
Lamellar body phospholipases hydrolyse dipalmitoyl phosphatidylcholine ( containing 10 % phosphatidylglycerol and thus approximating the phospholipid composition of mature lamellar bodies ) at only 1 % of their rate of hydrolysis of unsaturated molecules ( Tables 1 and 2 ) . - pubmed
The synthesis of peroxides was estimated by the measurement of leukotriene B4 and C4 ( LTB4 , LTC4 ) . - pubmed
Axial and sagittal T1 - weighted images of the lumbar spine were obtained , pre- and post - Gd - DTPA ( 0.1 mmol / kg ) administration . - pubmed
Omeprazole inhibits ATPase activity also in isolated plasma - membrane vesicles . - pubmed
Progestins were dissolved in propylene glycol and IV injected through an indwelling jugular catheter . - pubmed
alpha - Naphthoflavone , a known inhibitor of P4501A - related activities , did not affect these procarcinogen activation in fetal liver microsomes . - pubmed
Furthermore , we analysed the effects of the non - selective ( A / B ) endothelin receptor antagonist , bosentan ( 6 and 100 mg kg-1 day-1 ) on mean arterial blood pressure , water and sodium excretion and glomerular filtration rate . - pubmed
Moreover DNA cleavage can be identified by electrophoresis and by specific labeling using in situ nucleotidyltransferase assay ( ISNT ) , terminal deoxynucleotidyltransferase - mediated dUTP nick - end labeling technique ( Tunel ) , or by Elisa . - pubmed
[ Percutaneous alcohol instillation therapy ( PAI ) and chemoembolization in extensive hepatocellular carcinoma ] . - pubmed
Following measurements were done : quantitative computed tomography , radiological estimation of thoracic and lumbal spinal column and hands , in serum total alkaline phosphatase , calcium , phosphate , in urine excretion of calcium , phosphate and creatinine . - pubmed
Nitric oxide ( NO ) , a gaseous molecule synthesized in the arteriolar endothelium from the amino acid L - arginine ( L - arg ) , has been identified as the previously described Endothelium - Derived Relaxing Factor ( EDRF ): nitroderivatives such as nitroglycerin are known to induce vasodilation via NO release . - pubmed
The first step of glycosylphosphatidylinositol biosynthesis is mediated by a complex of PIG - A , PIG - H , PIG - C and GPI1 . - pubmed
Biosynthesis of glycosylphosphatidylinositol ( GPI ) is initiated by transfer of N - acetylglucosamine ( GlcNAc ) from UDP - GlcNAc to phosphatidylinositol ( PI ) . - pubmed
PIG - L , which is involved in the second step in GPI synthesis , GlcNAc - PI de - N - acetylation , did not associate with the isolated complex . - pubmed
The protein complex had GPI - GlcNAc transferase ( GPI - GnT ) activity in vitro , but did not mediate the second reaction . - pubmed
These results suggest that GPI - GnT recognizes the fatty acyl chains of PI . - pubmed
Apoptosis in 30 autologous PBSC harvests mobilised by cyclophosphamide + G - CSF or standard chemotherapy + G - CSF was analysed immediately after collection by dual - colour flow cytometry with CD34 PE and annexin V FITC . - pubmed
Cyclophosphamide + G - CSF significantly increased the yield of CD34 + cells but did not increase apoptosis . - pubmed
The heterologously expressed synthases produce ( + ) - bornyl diphosphate , 1 , 8 - cineole , and ( + ) - sabinene , respectively , as their major products from geranyl diphosphate . - pubmed
The bornyl diphosphate synthase also produces significant amounts of ( + ) - alpha - pinene , ( + ) - camphene , and ( + /-)- limonene . - pubmed
The 1,8 - cineole synthase produces significant amounts of ( + ) - and ( -)- alpha - pinene , ( + ) - and ( -)- beta - pinene , myrcene and ( + ) - sabinene , and the ( + ) - sabinene synthase produces significant quantities of gamma - terpinene and terpinolene . - pubmed
Plasmodial hydroperoxide metabolism displays similarities to the mammalian system in comprising glutathione biosynthesis , glutathione reductase , and at least one glutathione peroxidase homolog having the active site selenocysteine replaced by cysteine . - pubmed
This observation indicates that PrRP may regulate oxytocin secretion . - pubmed
These results suggest that the PrRP acts as a neuro - modulator of the function of magnocellular neurons , especially oxytocin neurons , in the brain . - pubmed
In view of the conflicting data in the literature concerning the effect of calcium channel antagonists on autonomic balance , we evaluated the effect of verapamil on heart rate and blood pressure variability , and on baroreflex sensitivity . - pubmed
Hyperbaric oxygen treatment attenuates cytokine induction after massive hemorrhage . - pubmed
We investigated the effect of hyperbaric oxygen treatment ( HBO ) on cytokine induction after hemorrhage , because hypoxia induces cytokines in vitro . - pubmed
Time course changes in plasma endotoxin level , arterial ketone body ratio ( AKBR ) , serum tumor necrosis factor ( TNF ) , interleukin-6 ( IL-6 ) , and their hepatic mRNA were detected in the three groups . - pubmed
HBO significantly attenuated decreases in AKBR after hemorrhage with a significant reduction of mortality and cytokine induction . - pubmed
These results indicate that HBO attenuated the cytokine induction after hemorrhage by improving liver ischemia , and they suggest that tissue hypoxia may be responsible , at least in part , for cytokine induction after massive hemorrhage . - pubmed
Reaction with , and fine structural recognition of polyamines by human IgE antibodies . - pubmed
Human IgE antibodies from nine allergic subjects were found to react with poly - L - lysine ( PLL ) and other polyamines . - pubmed
Radioimmunoassay inhibition studies indicated that the two amino groups , but not the carboxyl , in lysine contributed to the antibody binding and 4 - aminomethyl-1,8 - octanediamine , a compound containing three primary amino groups , was a better inhibitor than compounds containing only two primary amino groups . - pubmed
Ethylamine showed weak but clear inhibition indicating that even a single amino group could bind to the antibody combining site . - pubmed
Inhibition studies with compounds containing an amino and a carboxyl group at different distances revealed that an adjacent carboxyl group interfered with recognition of the amino group by some IgE antibodies . - pubmed
IgE binding to PLL was examined at different pHs and ionic strengths . - pubmed
These results indicated that binding of specific antibody to polyamine was due to electrostatic interactions of positively charged amino groups in the polyamine with the antibody combining site . - pubmed
This non - processed lipase had 50 % residual activity after 2 h at 50 degrees C , while the residual activity of the processed lipases was only 10 % after 30 - 45 min of incubation at 50 degrees C. The CD spectrum of the non - processed type of lipase at 222 nm was almost unchanged by heating , suggesting that this group of lipases had a very rigid structure and that the peptide bond between the A- and B - chain contributed to maintain this rigid structure . - pubmed
On the other hand , the length of the sugar chain bound to the lipase had no effect on the thermal stability . - pubmed
Cyclosporine impairs the guanylyl cyclase activity of the natriuretic peptide receptor in the glomerulus . - pubmed
The guanylyl cyclase activity in the glomerulus from the CsA group was attenuated compared with that from the control . - pubmed
Direct application of CsA to normal glomerular membrane completely abolished the ANP - induced guanylyl cyclase activation . - pubmed
Binding studies , using( 125 ) I - ANP , revealed that B(max)was decreased in the CsA group , while K(d)was not affected in the glomerulus . - pubmed
These results indicate that CsA impairs the guanylyl cyclase activity mainly in the glomerulus by the decrease in NPR population and/or by direct inhibition , suggesting that the ANP / NPR system might be involved in CsA - induced nephrotoxicity . - pubmed
BACKGROUND : Na + : HCO3 - cotransporters mediate the transport of HCO3 - into or out of the cell . - pubmed
Expression studies in oocytes indicated that NBC-3 mediates Na - dependent HCO3 - cotransport . - pubmed
CONCLUSIONS : Based on its distribution and electroneutrality , we propose that kNBC-3 mediates the transport of HCO3 - into the cells . - pubmed
NO synthase ( NOS ) catalyses the conversion of L - arginine to L - citrulline in the presence of oxygen and NADPH - diaphorase ( NADPH - d ) . - pubmed
After the hearts were excised , the NADPH - d co - enzyme was visualized by a histochemical method and the endothelial isoform of NOS was localized by immunohistochemistry . - pubmed
The eNOS visualization , which involved immunoprecipitation , and the NADPH - d visualization , which involved histochemical staining , were both diminished in endothelial cells of the vascular wall of diabetic hearts , compared to non - diabetic hearts . - pubmed
The residues of additives and other chemicals in 23 kinds of silicone rubber products for food contact use , including nipples , packing and spatulas , were investigated by GC / MS . - pubmed
Proteoglycan ( PG ) content of cartilage was measured by a dimethylmethylene blue assay of papain digests . - pubmed
Ribonucleotide reductase ( RNR ) is the enzyme performing de novo production of the four deoxyribonucleotides needed for DNA synthesis . - pubmed
The smaller of the homodimers , denoted R2 , contains a di - iron carboxylate site which , upon reaction with molecular oxygen , generates a stable tyrosyl radical needed for catalysis . - pubmed
The present structure provides evidence that manganese is not oxidized by the protein , in agreement with recent biochemical data , and that no obvious structural abnormalities are seen in the oxidized and reduced iron - containing forms , giving further support that the protein is indeed an iron - dependent RNR R2 . - pubmed
ammoniagenes R2 also reveals an additional water molecule bridging the radical and the iron site , which has not previously been seen in any other R2 structure and which might have important mechanistic implications . - pubmed
Appropriate recommendations and correct interpretations of the results of routine thyroid scans and the usefulness of other scintigraphic studies with 111In - octreoscan , 99mTc - MIBI , 99mTc - DMSA , 18FDG and radiolabeled monoclonal anti - carcinoembryonic antigen antibodies were discussed in the light of the present thyroid diagnostic studies . - pubmed
aegypti KAT ( AeKAT ) has four potential N - glycosylation sites ( Asn - Xxx - Trp / Ser ) and a typical mitochondrial leader sequence consisting of 49 amino acids at its NH(2)-terminus with a putative cleavage site between Met-49 and Ser-50 . - pubmed
Using PEI vector , both pancreatic primary tumor growth and metastatic tumor growth were also significantly slackened as compared with both LacZ - treated and untreated control groups ( P & lt ; 0.02 ) . - pubmed
Three points are discussed : ( 1 ) the different conformations of CsA and the question of its biologically active form ; ( 2 ) the Fab - CsA recognition mechanism ; and ( 3 ) the relationship between structure and binding properties of CsA analogs . - pubmed
By use of basic coordination chemistry , 4 -( 3 - quinolinoazo ) hydroxybenzoic acid ( QABA ) and derivatives were successfully conjugated to ovalbumin , bovine serum albumin , and alkaline phosphatase at a site that was distinct from the metal binding site . - pubmed
A single amino acid alteration in cytoplasmic domain determines IL-2 promoter activation by ligation of CD28 but not inducible costimulator ( ICOS ) . - pubmed
Together , these observations lead us to believe that the difference of a single amino acid , which affects Grb2 binding ability , may define a functional difference between the CD28- and ICOS - mediated costimulatory signals . - pubmed
Proliferation , cytotoxicity , and cytokine secretion were measured by [ ( 3 ) H ] thymidine incorporation , ( 51 ) Cr release , and enzyme - linked immunosorbent assay , respectively . - pubmed
The specificity of the drug T cell receptor interaction was studied using CBZ metabolites and other structurally related compounds . - pubmed
The T cell receptor of certain clones could accommodate some CBZ metabolites , but no cross - reactivity was seen with other anticonvulsants or structural analogs . - pubmed
C - reactive protein ( CRP ) , a marker of systemic inflammation and an independent predictor of cardiovascular mortality in the general population , and asymmetric dimethylarginine ( ADMA ) , an endogenous inhibitor of NO synthase , are important risk factors for cardiovascular disease and mortality in the ESRD population . - pubmed
Both CRP and ADMA have been shown to be associated with increases in the incidence and progression of atherosclerotic lesions in carotid arteries , as evaluated by high - resolution Doppler ultrasonography . - pubmed
At E18 and P0 - P2 , the in vitro frequency decreased by around 50 % under hypoxia , increased by 40 - 50 % under noradrenaline or substance P and was permanently depressed by the pontine A5 areas . - pubmed
At E16 however , hypoxia had no effects , both noradrenaline and substance P drastically increased the frequency and area A5 inhibition was not expressed at this time . - pubmed
The average drop in total cholesterol was 24.8 mg / dL ( 9.9 % ) , LDL 15.3 mg / dL ( 11.4 % ) , and triglycerides 38 mg / dL ( 9.9 % ) . - pubmed
The effects of ribonuclease and other basic proteins on the incorporation of C14 - L - leucine into liver proteins in vitro . - pubmed
The NADPH - oxidase is a plasma membrane enzyme complex that enables phagocytes to generate superoxide in order to kill invading pathogens , a critical step in the host defense against infections . - pubmed
The oxidase transfers electrons from cytosolic NADPH to extracellular oxygen , a process that requires concomitant H+ extrusion through depolarization - activated H+ channels . - pubmed
Whether H + fluxes are mediated by the oxidase itself is controversial , but there is a general agreement that the oxidase and H + channel are intimately connected . - pubmed
Oxidase activation evokes profound changes in whole - cell H + current ( IH ) , causing an approximately -40 - mV shift in the activation threshold that leads to the appearance of inward IH . - pubmed
Run - down of both IH and Ie was delayed , and sometimes prevented , by cytosolic ATP and GTP - gamma - S. A good correlation was observed between the amplitude of Ie and both inward and outward IH when a stable driving force for e- was imposed . - pubmed
Combined application of NADPH and DPI reduced the inward IH amplitude , even in the absence of concomitant oxidase activity . - pubmed
Etoposide , a substrate of both P - glycoprotein and CYP3A , was used as a model drug . - pubmed
Of special significance from a clinical perspective is also CD26 effect on glucose metabolism through its DPP IV activity and its potential role as a therapeutic target in diabetes . - pubmed
A number of experimental and clinical studies have demonstrated the beneficial effects of trimetazidine , a drug that inhibits the long - chain mitochondrial 3 - ketoacyl coenzyme A thiolase enzyme in the myocyte , resulting in a shift from fatty acid oxidation to glucose oxidation . - pubmed
The products of heme catalysis , biliverdin / bilirubin , carbon monoxide ( CO ) , and iron ( that induces apoferritin ) mediate the beneficial effects of HO-1 . - pubmed
HO-1 was up - regulated by cobalt - protoporphyrin ( 5 mg / kg , intraperitoneally ) . - pubmed
RESULTS : Cobalt - protoporphyrin treatment resulted in significant up - regulation of HO-1 protein in mucosal and submucosal cells . - pubmed
CONCLUSIONS : We conclude that heightened HO-1 expression or administration of biliverdin ameliorates dextran sodium sulfate - induced experimental colitis . - pubmed
Novel therapeutic strategies based on HO-1 and/or biliverdin administration may have use in inflammatory bowel disease . - pubmed
Bioactivity in vitro tests were excellent : ( a ) rapid and pronounced formation of hydroxyapatite occurred when the scaffolds were immersed in simulated body fluid ( SBF ) , and ( b ) outstanding proliferation of osteoblasts was registered . - pubmed
Amyloid deposition was histologically confirmed , and severe inflammatory activity was found in and at the margins of the amyloidomas , the latter correlating in CT with the halo sign . - pubmed
The increase that occurred during the administration partially remained in the Ca ( 2 +)-free medium and was blocked by 2 - aminoethoxydiphenyl borate ( 2 - APB ) , an IP ( 3 )- receptor blocker , indicating that the source of Ca ( 2 + ) for the increase could be ascribed to the intracellular store . - pubmed
The effects on the [ Ca ( 2 +)]( i ) were blocked by pretreatment with pyridoxal phosphate-6 - azophenyl-2 ' , 4 ' disulfonic acid , a P2 - receptor antagonist . - pubmed
About 65 % of thymus BuChE activity was extracted with a saline buffer and 30 % with 1 % Triton X-100 . - pubmed
Sedimentation analyses and phenyl - agarose chromatography showed that thymus contained amphiphilic BuChE monomers ( G(1)(A),44 % ) and dimers ( G(2)(A),33 % ) , and hydrophilic tetramers ( G(4)(H),23 % ) . - pubmed
To identify specific substrates , a mutant of PKCalpha ( M417A ) was constructed at the ATP binding site such that it could bind a sterically large ATP analogue derivatized through the N6 amino group of adenosine ( [ gamma-32P ]- N6 - phenyl - ATP ) . - pubmed
Because this analogue could be utilized by the mutant kinase but not by wild - type PKCalpha ( or presumably other protein kinase ) to phosphorylate peptide or protein substrates , 32P - labeled products were the direct result of the mutant PKCalpha . - pubmed
We investigated five functional polymorphisms in the transforming growth factor beta1 gene ( -800 G / A , -509 C / T , codon 10 Leu / Pro , codon 25 Arg / Pro and codon 263 Thr / Ile ) in relation to arterial stiffness in a population - based study . - pubmed
The results of this study show that the TGF - beta1 -800 G / A , -509 C / T , codon 10 Leu / Pro and codon 25 Arg / Pro polymorphisms are not associated with arterial stiffness . - pubmed
Specifically , hypoxia markedly increased LOX protein expression ; however , catalytic activity ( beta - aminopropionitrile inhibitable hydrogen peroxide production ) was significantly reduced under hypoxic conditions . - pubmed
This work demonstrates that both hypoxia and reoxygenation are necessary for LOX catalytic activity which facilitates breast cancer cell migration through a hydrogen peroxide - mediated mechanism ; thereby illuminating a potentially novel mechanism by which poorly invasive cancer cells can obtain metastatic competency . - pubmed
Immobilization of the pronase enzymes facilitated their removal from the glycopeptide preparations , and was found to prevent enzyme autolysis while leaving the proteolytic activities of pronase intact . - pubmed
Monitoring the progress of glycoprotein digestion over time allowed detailed tracking of successive amino acid cleavages about the sites of glycan attachment , and provided a more complete protein glycosylation profile than any single representative time point . - pubmed
Separation of beta - receptor blockers and analogs by capillary liquid chromatography ( CLC ) and pressurized capillary electrochromatography ( pCEC ) using a vancomycin chiral stationary phase column . - pubmed
Enantiomeric separation of chiral pharmaceuticals was carried out by means of in capillary liquid chromatography ( CLC ) and pressurized capillary electrochromatography ( pCEC ) using a vancomycin chiral stationary phase ( CSP ) . - pubmed
Enantiomeric resolution of fifteen beta3 - receptor blockers and analogs was studied by polar organic CLC mode and reversed - phase pCEC mode using mobile phases containing methanol - isopropanol - acetic acid - triethylamine and TEAA buffer - methanol , respectively . - pubmed
Good enantiomeric resolution was achieved by CLC mode for propranolol , celiprolol , esmolol , bisoprolol , atenolol , metoprolol and carteolol using methanol - isopropanol - acetic acid - triethylamine ( 70:30:0.05:0.05 , v / v / v / v ) as mobile phase and for clenbuterol , bambuterol , terbutaline , and salbutamol using methanol - isopropanol - acetic acid - triethylamine ( 50:50:0.05:005 or 50:50 : 0.025:0.05 , v / v / v / v ) as mobile phase . - pubmed
Acetone extract ( 92microg GAE / mL ) increased QR activity 2.8 - fold , while ethanol ( 120microg GAE / mL ) and water ( 1000microg GAE / mL ) extracts increased QR activity by 1.35- and 1.56 - fold , respectively . - pubmed
Peritoneal fluid samples were centrifuged at 600 x g for 10 min and 4 degrees C , and the supernatant was incubated at 56 degrees C for 30 min in a water bath to inactivate the complement protein . - pubmed
After a simple step of protein precipitation using methanol , plasma samples were analyzed by reverse phase high - performance liquid chromatography - electrospray ionization - tandem mass spectrometry ( HPLC - ESI - MS / MS ) with valsartan as the internal standard ( I.S. ) - pubmed
valsartan were separated on a Venusil MP C18 analytical column using methanol-10mM ammonium acetate ( 75:25 , v / v , pH 3.0 ) as mobile phase at a flow rate of 1.0 mL / min . - pubmed
Interaction of [ Rh ( 2 )( O ( 2 ) CCH ( 3 ))( 4 )( H ( 2 ) O )( 2 ) ] and [ Rh ( 2 )( O ( 2 ) CCH ( OH ) Ph )( 2 )( phen )( 2 )( H ( 2 ) O )( 2 )]( O ( 2 ) C - CH ( OH ) Ph )( 2 ) With Sulfhydryl Compounds and Ceruloplasmin . - pubmed
The interaction of binuclear rhodium ( II ) complexes [ Rh ( 2 )( OOCCH ( 3 ))( 4 )( H ( 2 ) O )( 2 ) ] , [ Rh ( 2 ){ OOCCH ( OH ) Ph }( 2 )( phen )( 2 )( H ( 2 ) O )( 2 ) ] { OOCCH ( OH ) Ph }( 2 ) , [ Rh ( 2 )( OOCCH ( 3 ))( 2 )( bpy )( 2 )( H ( 2 ) O )( 2 )]( OOCCH ( 3 ))( 2 ) and [ Rh ( 2 ) Cl ( 2 )( OOCMe )( 2 )( bpy )( 2 )]( 3H ( 2 ) O ) with ceruloplasmin , cysteine , glutathione and coenzyme A have been investigated using . - pubmed
Complex [ Rh ( 2 ){ OOCCH ( OH ) Ph }( 2 )( phen )( 2 )( H ( 2 ) O )( 2 ) ] strongly changes structure of ceruloplasmin leading to the decrease of of alpha - helix content and loss of oxidase activity . - pubmed
Directly relating reduction energies of gaseous Eu ( H2O ) n ( 3 + ) , n = 55 - 140 , to aqueous solution : the absolute SHE potential and real proton solvation energy . - pubmed
In solution , half - cell potentials are measured relative to other half - cells resulting in a ladder of thermodynamic values that is anchored to the standard hydrogen electrode ( SHE ) , which is assigned an arbitrary value of exactly 0 V. A new method for measuring the absolute SHE potential is introduced in which reduction energies of Eu ( H ( 2 ) O )( n )( 3 + ) , from n = 55 to 140 , are extrapolated as a function of the geometric dependence of the cluster reduction energy to infinite size . - pubmed
Losses of antioxidant activity , TP , flavanol monomers , and procyanidins were associated with an increased extractable pH of the baked cakes . - pubmed
The adsorbents from pyrolusite - supplemented sludges performed better in dye removal than those without mineral addition , with the carbon from pyrolusite - augmented sludge T2 presenting a significant increase in maximum adsorption capacity of 50mg / g. The properties of the adsorbents were improved during pyrolusite - catalyzed pyrolysis via enhancement of mesopore production , thus the mesopore channels may provide fast mass transfer for large molecules like dyes . - pubmed
Estimates of landfill gas ( LFG ) collection efficiency are required to estimate methane emissions and the environmental performance of a solid waste landfill . - pubmed
Apolipoprotein CIII bound to apoB - containing lipoproteins is associated with small , dense LDL independent of plasma triglyceride levels in healthy men . - pubmed
OBJECTIVE : Plasma levels of both apolipoprotein CIII ( apoCIII ) and small , dense LDL particles are associated with plasma triglyceride ( TG ) concentrations , and are markers for cardiovascular disease ( CVD ) risk . - pubmed
Stepwise multiple regression analysis showed that LpB : CIII was significantly associated with small , dense LDL ( LDL3+LDL4 ) levels after adjusting for age , BMI , plasma levels of TG , apoB , and HDL - cholesterol . - pubmed
The objective of this study was to determine the genotoxic activity of water after UV / H ( 2 ) O ( 2 ) oxidation and GAC filtration . - pubmed
Pre - treated surface water from three locations was treated with UV / H ( 2 ) O ( 2 ) with medium pressure ( MP ) lamps and passed through granulated activated carbon ( GAC ) . - pubmed
The results indicate that UV / H ( 2 ) O ( 2 ) treatment may lead to the formation of genotoxic by - products , which can be removed by subsequent GAC filtration . - pubmed
One of the more prevalent views is that immunodominance is driven by passive T cell competition for space on antigen presenting cells ( APCs ) or for access to specific MHC : epitope complexes on the surface of APCs . - pubmed
On the other hand , the non - natural amino acids are not metabolized but are taken up through both the L - type transporter and the energy - dependent A - type transporter ( 7 ) . - pubmed
Sequencing of the mevalonate kinase gene revealed 2 mutations , leading to amino acid substitutions : one already described ( V3771 ) and R40W : never reported before . - pubmed
Four biopsies were taken during bisphosphonate therapy and one biopsy was taken after one year of strontium ranelate ( SR ) treatment . - pubmed
CONCLUSIONS / SIGNIFICANCE : Microstructural data revealed a notable increase in bone volume fraction after one year of SR treatment compared to the bisphosphonate treatment period . - pubmed
Administration of SR following bisphosphonates led to a maintained mineralization profile with an uptake of strontium on the bone surface level . - pubmed
This was achieved by dispersion polymerization of methacrylic acid ( MAA ) , polyethylene glycol ( PEG ) and different chitosan ( CS ) grades in the presence of cross linking agent ethylene dimethacrylate ( EDMA ) and polymer initiator potassium persulphate . - pubmed
Method development was carried out by varying formulation parameters such as type of CS , ratio of PEG to CS , quantity of solvent and polymer initiator . - pubmed
These findings closely resembled the distribution of signal loss on T2 - weighted MRI seen after SPIO injection reflecting intravascular accumulation of iron particles trapped in vessel thrombi as confirmed histologically . - pubmed
Proapoptotic protein Bim is differentially required during thymic clonal deletion to ubiquitous versus tissue - restricted antigens . - pubmed
The proapoptotic Bcl-2 family member Bim is important for negative selection by inducing apoptosis in thymocytes receiving a strong signal through their antigen receptor . - pubmed
Unlike UbA , clonal deletion to tissue - restricted antigens ( TRAs ) requires positive selection and CCR7 - mediated migration to the medulla . - pubmed
Serum NGAL correlated with age , haematocrit , leukocyte , platelet and erythrocyte count , eGFR , creatinine , fasting glucose , HbA ( 1c ) , fibrinogen , SBP , and diabetes duration . - pubmed
In multiple regression analysis kidney function ( eGFR , creatinine clearance or creatinine ) , cystatin C and SBP were predictors of serum NGAL . - pubmed
In the present paper , xanthohumol exhibited more - potent activity in inhibiting platelet aggregation stimulated by collagen . - pubmed
Xanthohumol inhibited platelet activation accompanied by relative [ Ca ( 2 +)]( i ) mobilization , thromboxane A ( 2 ) formation , hydroxyl radical ( OH ( ) ) formation , and phospholipase C ( PLC ) gamma2 , protein kinase C ( PKC ) , mitogen - activated protein kinase ( MAPK ) , and Akt phosphorylation . - pubmed
Neither SQ22536 , an inhibitor of adenylate cyclase , nor ODQ , an inhibitor of guanylate cyclase , reversed the xanthohumol - mediated inhibitory effect on platelet aggregation . - pubmed
This study demonstrates for the first time that xanthohumol possesses potent antiplatelet activity which may initially inhibit the PI3 - kinase / Akt , p38 MAPK , and PLCgamma2 - PKC cascades , followed by inhibition of the thromboxane A ( 2 ) formation , thereby leading to inhibition of [ Ca ( 2 +)]( i ) and finally inhibition of platelet aggregation . - pubmed
The optical response of LCs changed from a bright to dark appearance when an aqueous solution of lipase was in contact with a nematic LC , 4 - cyano-4 '- pentylbiphenyl ( 5CB ) , that was doped with glyceryl trioleate , which is a glyceride that can be enzymatically hydrolyzed by lipase . - pubmed
We did not observe a bright - to - dark shift in the optical appearance of LCs when pure 5CB was immersed into the lipase solution . - pubmed
Moreover , we further confirmed the specificity of the enzymatic reaction by transferring an aqueous buffer solution not containing an analyte , or with bovine serum albumin ( BSA ) or trypsin onto the interface of aqueous solutions and the glyceryl trioleate - doped 5CB , which did not produce any distinctive contrast in the optical appearance . - pubmed
Body weight , height , BMI , and waist circumference were recorded and fasting serum levels of glucose , insulin , 25 ( OH ) D , and parathyroid hormone ( PTH ) were measured . - pubmed
The mean level of 25 ( OH ) D was higher in the SGA group ( 26.2 + -10 vs. 17.2 + -7 ng / ml , p<0.01 ) but that of PTH was no different . - pubmed
In the SGA group , 25 ( OH ) D was correlated with HOMA - beta% but not with HOMA - IR or insulin . - pubmed
Using preclinical models and patients ' samples we recently reported that the emergence of KRAS mutations but also KRAS amplification is associated with acquired resistance to the EGFR inhibitors cetuximab or panitumumab . - pubmed
Blockade of MORs using 1 or 3 mug of H - d - Phe - Cys - Tyr - d - Trp - Arg - Thr - Pen - Thr - NH2 within the dorsal striatum decreased mating during the cohabitation period and inhibited partner preference formation . - pubmed
AIMS : While there is thought to be an association between glucose and lipid metabolism , it is largely unknown whether apolipoprotein B and non - high density lipoprotein ( HDL ) cholesterol are associated with the development of Type 2 diabetes . - pubmed
RESULTS : Apolipoprotein B and non - HDL cholesterol levels showed a strong association with the development of Type 2 diabetes compared with conventional lipid measurements and their ratios [ hazard ratio per 1 sd ; 1.39 ( 95 % CI 1.37 - 1.42 ) and 1.38 ( 95 % CI 1.35 - 1.40 ) , respectively ; both P & lt ; 0.001 ] . - pubmed
The Kaplan - Meier survival curve demonstrated that Type 2 diabetes developed more frequently as apolipoprotein B or non - HDL cholesterol levels increased across quartiles ( both P & lt ; 0.001 ) . - pubmed
In multivariate Cox regression analyses , both apolipoprotein B and non - HDL cholesterol were associated with the development of Type 2 diabetes , independent of other risk factor including age , sex , waist circumference , family history of diabetes , fasting serum glucose and insulin levels , HbA1c , systolic blood pressure and other conventional lipid measurements [ hazard ratio per 1 sd ; 1.14 ( 95 % CI 1.11 - 1.18 ) and 1.13 ( 95 % CI 1.10 - 1.16 ) , respectively ; both P & lt ; 0.001 ] . - pubmed
Berberine protects against palmitate - induced endothelial dysfunction : involvements of upregulation of AMPK and eNOS and downregulation of NOX4 . - pubmed
The expressions of eNOS were significantly increased , while NOX4 protein expression was decreased in berberine - treated HUVECs . - pubmed
Moreover , berberine upregulated the protein expression of AMPK and p - AMPK in palmitate - treated HUVECs , but had no effect on the levels of Akt . - pubmed
Therefore , berberine ameliorates palmitate - induced endothelial dysfunction by upregulating eNOS expression and downregulating expression of NOX4 . - pubmed
This regulatory effect of berberine may be related to the activation of AMPK . - pubmed
We determined the value of estimated GFR ( eGFR ) as a threshold for the increase of plasma cFGF23 ( C - terminal ) and intact fibroblast growth factor-23 ( iFGF23 ) ( intact ) concentrations in the course of chronic kidney disease ( CKD ) and compared this eGFR value with values related to increased serum intact parathyroid hormone ( iPTH ) or phosphorus concentrations in an elderly population . - pubmed
RESULTS : We found that genistein treatment enhanced ECFCs ' migration and proliferation , which was accompanied by increases in the expression of ILK , alpha - parvin , F - actin , and phospholylation of ERK 1 / 2 signaling . - pubmed
Transplantation of genistein - stimulates ECFCs ( GS - ECFCs ) into myocardial ischemic sites in vivo induced cellular proliferation and secretion of angiogenic cytokines at the ischemic sites and thereby enhanced neovascularization and decreased myocardial fibrosis as well as improved cardiac function , as shown by echocardiography . - pubmed
Furthermore , proline - rich antimicrobial peptides display the ability to not only modulate the immune system via cytokine activity or angiogenesis but also possess properties of penetrating cell membranes and crossing the blood brain barrier suggesting a role as potential novel carriers . - pubmed
Enzyme activity and transcript levels of HEMA1 , GSA and ALAD for ALA synthesis greatly decreased in ALA- and OF - treated plants . - pubmed
Transcript levels of PPO1 , CHLH , CHLI , and PORB genes involving Mg - porphyrin synthesis continuously decreased in ALA- and OF - treated plants , with greater decreases in ALA - treated plants . - pubmed
By contrast , up - regulation of FC2 and HO2 genes in Fe - porphyrin branch was noticeable in ALA and OF - treated plants 1 day and 2 days after the treatments , respectively . - pubmed
Decreased transcript levels of nuclear - encoded genes Lhcb1 , Lhcb6 , and RbcS were accompanied by disappearance of MgProto IX in ALA- and OF - treated plants after 2 days of the treatments . - pubmed
The up - regulation of FC2 and HO2 also appears to compensate for the photodynamic stress - induced damage . - pubmed
Nigral and striatal redox balance , was studied by means of biochemical indicators such as malondialdehyde ( MDA ) , nitric oxide ( NO ) and the catalase enzymatic activity ( CAT EA ) . - pubmed
Biochemical studies revealed that after 48 - h PPN neurotoxic injury , the CAT EA showed a significant increase in the subtantia nigra pars compacta ( SNpc ) ( p<0.05 ) . - pubmed
This significant increase of CAT EA persisted in the nigral tissue ( p<0.001 ) and reached the striatal tissue ( p<0.001 ) seven days after PPN injury . - pubmed
Also at seven days post - injury PPN , increased concentrations of MDA ( p<0.01 ) and a tendency to decrease in the concentrations of NO in both structures ( SNpc and striatum ) were found . - pubmed
In particular , the role of glycated hemoglobin ( HbA1c ) and oral glucose tolerance testing ( OGTT ) will be reviewed . - pubmed
BMP-1 digestion of DSPP - derived proteins extracted from dental pulp did not generate new DPP bands on sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( indicating an absence of intact DSPP ) , although the results suggested another BMP-1 cleavage site within DSP . - pubmed
Its amino acid composition was similar to DSP . - pubmed
RESULTS : Dabigatran altered the prothrombin time , activated partial thromboplastin time and thrombin time , and the measurement of intrinsic and extrinsic factor levels . - pubmed
Conversely , the addition of dabigatran falsely corrected an abnormal activated protein C resistance ratio . - pubmed
MEASUREMENTS : Between 2011 and 2013 , fasting venous samples were analyzed for 25 ( OH ) D , iPTH , alkaline phosphatase ( ALP ) , calcium , and phosphorus . - pubmed
Several anti - EGFR antibodies including cetuximab are used in clinics for treatment of EGFR - overexpressing colorectal and head and neck cancers but the efficiency of these antibodies is threatened by their large size and chimeric nature . - pubmed
Here , we aimed to define further the reactivity profile of CHB - associated antibodies towards Ro52p200 ( amino acid 200 - 239 ) . - pubmed
In particular , amino acid D233 appeared crucial to maternal antibody reactivity towards p200 . - pubmed
T Cells Encountering Myeloid Cells Programmed for Amino Acid - dependent Immunosuppression Use Rictor / mTORC2 Protein for Proliferative Checkpoint Decisions . - pubmed
Using a reduced complexity in vitro macrophage - T cell co - culture system , we show that macrophage arginase-1 is the only factor required by M2 macrophages to block T cells in G1 , and this effect is mediated by l - arginine elimination rather than metabolite generation . - pubmed
After 2 days of arginine deprivation , mTORC1 activity declined paralleling a selective down - regulation of SREBP target gene expression , whereas mRNAs involved in glycolysis , gluconeogenesis , and T cell activation were unaffected . - pubmed
Arginine deprivation - induced cell cycle arrest was mediated in part by Rictor / mTORC2 , providing evidence that this nutrient recognition pathway is a central component of how T cells measure environmental arginine . - pubmed
For those receiving atomoxetine , the summary pre - post ( e.g. - pubmed
The regulation of tumor suppressor protein , p53 , and estrogen receptor ( ERalpha ) by resveratrol in breast cancer cells . - pubmed
Previous studies from our laboratory have shown the tumor suppressor protein p53 and estrogen receptor - alpha ( ERalpha ) to be possible molecular targets for RES . - pubmed
In this study , the anti - estrogenic effects of RES were analyzed on the expression of ERalpha and p53 . - pubmed
Our studies indicate that RES caused a decrease in the levels of protein expression of p53 and ERalpha as compared to the control . - pubmed
RES , in conjunction with ICI 182,780 ( ICI ) , caused a down - regulation of both p53 and ERalpha as compared to the control . - pubmed
These observed effects on cell proliferation and regulation of both p53 and ERalpha by RES may lead to further understanding of the relationship between tumor suppressor proteins and steroid receptors in breast cancer cells . - pubmed
AIM : The aim of this study was to compare the effectiveness of 0.1 % levobupivacaine and 0.1 % ropivacaine with fentanyl as an adjuvant for epidural labor analgesia in terms of onset , duration , quality of analgesia , and degree of motor blockade . - pubmed
Camptothecin resistance is determined by the regulation of topoisomerase I degradation mediated by ubiquitin proteasome pathway . - pubmed
Proteasomal degradation of topoisomerase I ( topoI ) is one of the most remarkable cellular phenomena observed in response to camptothecin ( CPT ) . - pubmed
Importantly , the rate of topoI degradation is linked to CPT resistance . - pubmed
Here , we show that the Ku70 / Ku80 heterodimer binds with topoI , and that the DNA - dependent protein kinase ( DNA - PKcs ) phosphorylates topoI on serine 10 ( topoI - pS10 ) , which is subsequently ubiquitinated by BRCA1 . - pubmed
A higher basal level of topoI - pS10 ensures rapid topoI degradation leading to CPT resistance . - pubmed
Importantly , PTEN regulates DNA - PKcs kinase activity in this pathway and PTEN deletion ensures DNA - PKcs dependent higher topoI - pS10 , rapid topoI degradation and CPT resistance . - pubmed
Due to the color change property of cobalt chloride , the as - spun composite NFMs show obviously macroscopic color change from blue to pink as relative humidity ( RH ) increasing from 12.4 to 97.2 % . - pubmed
Owing to the above double sensitive mechanism on RH , the PA66 / CoCl2 composite NFM may show great potential applications from meticulous to coarse . - pubmed
Batch experiments showed that optimal K2FeO4 dosage and reaction time for sludge disintegration was 100mg / g suspended solids ( SS ) and 24h , respectively . - pubmed
Sgp130 , a natural antagonist of IL-6 trans - signaling , and sex hormones , especially testosterone and estradiol , are prominently considered as anti - atherosclerotic . - pubmed
Serum concentrations of hs - CRP , testosterone , estradiol , and other routine biochemical markers were also quantified . - pubmed
Besides , we observed the effects of testosterone and estradiol on sgp130 in HUVECs . - pubmed
In addition , multiple regression analysis indicated that serum sgp130 was positively associated with estradiol ( beta - coefficient = 0.450 , p & lt ; 0.001 ) and T / E2 ratio ( beta - coefficient = 0.257 , p = 0.001 ) . - pubmed
Furthermore , basic studies found that supernatant sgp130 levels of HUVECs were significantly increased by the cooperativity of testosterone and estradiol . - pubmed
ECM were extracted with methanol , precipitates were separated by centrifugation and metabolite production of individual embryo was analysed by LC - MS ( the positive ion mode ) . - pubmed
Changes in urinary albumin excretion rate ( UAER ) , serum malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) activity , urine 8 - hydroxyl deoxyguanosine ( 8 - OHdG ) , serum creatinine , estimated glomerular filtration rate , and lipid profile before and after 24 weeks of follow - up were examined and compared . - pubmed
SOD activity decreased significantly after 24 weeks in the SCH group ( p & lt ; 0.05 ) . - pubmed
The levels of mutant K - RAS and mutant N - RAS are rapidly reduced in a Beclin1 / ATG5 -dependent fashion by the irreversible ERBB1 / 2 / 4 inhibitor neratinib . - pubmed
The FDA approved irreversible inhibitor of ERBB1 / 2 / 4 , neratinib , was recently shown to rapidly down - regulate the expression of ERBB1 / 2 / 4 as well as the levels of c - MET and mutant K - RAS via autophagic degradation . - pubmed
In the present studies , in a dose - dependent fashion , neratinib reduced the expression levels of mutant K - RAS or of mutant N - RAS , which was augmented in an additive to greater than additive fashion by the HDAC inhibitors sodium valproate and AR42 . - pubmed
Neratinib could reduce PDGFRalpha levels in GBM cells , that was enhanced by sodium valproate . - pubmed
Knock down of Beclin1 or of ATG5 prevented neratinib and neratinib combined with sodium valproate / AR42 from reducing the expression of mutant N - RAS in established PDX and fresh PDX models of ovarian cancer and melanoma , respectively . - pubmed
Neratinib and the drug combinations caused the co - localization of mutant RAS proteins and ERBB2 with Beclin1 and cathepsin B. - pubmed
Collectively , our data reinforce the concept that the irreversible ERBB1 / 2 / 4 inhibitor neratinib has the potential for use in the treatment of tumors expressing mutant RAS proteins . - pubmed
The structure , dynamics , and ozone signal of the Quasi - Biennial Oscillation produced by the 35 - year NASA MERRA-2 ( Modern - Era Retrospective Analysis for Research and Applications ) reanalysis are examined based on monthly mean output . - pubmed
AIM AND OBJECTIVE : The multi - component condensation of benzil , primary amines , ammonium acetate and various aldehydes was efficiently catalyzed using cobalt oxide nanoparticles under ultrasonic irradiation . - pubmed
In addition , the catalytic influence of the nanocatalyst was examined in the one - pot reaction of primary amines , benzil , ammonium acetate and diverse aromatic aldehydes under ultrasonic irradiation . - pubmed
Discovery of MK-8282 as a Potent G - Protein - Coupled Receptor 119 Agonist for the Treatment of Type 2 Diabetes . - pubmed
G - Protein coupled receptor 119 ( GPR119 ) is a GPCR target at which agonists have demonstrated glucose - dependent insulin secretion and shows beneficial effects on glycemic control . - pubmed
The overall aim of this study was to evaluate whether iodine-131 radiolabeled monoclonal antibody ( mAb ) targeting programmed death - ligand 1 ( PD - L1 ) can be used for imaging of PD - L1 expression noninvasively in vivo and playing synergistic effect combined with immunotherapy . - pubmed
Anti - PD - L1 mAb was radiolabeled with iodine-131 ( 131 I - PD - L1 mAb ) and was characterized in vitro . - pubmed
Fluoride - Releasing Effect of a Modified Resin Denture Containing S - PRG Fillers on Salivary Fluoride Retention : A Randomized Clinical Study . - pubmed
This study aimed to evaluate the clinical effect of a resin denture base containing 20wt% surface prereacted glass ionomer ( S - PRG ) fillers on saliva fluoride concentrations as a surrogate evaluation of caries control . - pubmed
A significant increase in salivary fluoride concentration in the S - PRG denture group was observed from baseline to day 1 , followed by a significant decrease to an approximate baseline level . - pubmed
The resin denture base containing 20wt% S - PRG fillers demonstrated an initial fluoride release that increased saliva fluoride concentrations , but the fluoride release from within the material was short lived . - pubmed
Intense near - infrared ( NIR ) emitting phosphors Ca5 ( PO4 ) 3 Cl : Eu2 + , Nd3 + were synthesized using a conventional solid - state reaction method and characterized using X - ray diffraction , reflectance , photoluminescence ( PL ) emission and PL excitation ( PLE ) spectroscopy , and PL lifetime measurements . - pubmed
We compared the AMH levels of the two groups and receiver operating characteristic ( ROC ) curve was used to determine the threshold for AMH in predicting the ovulatory response to CC therapy . - pubmed
AMH values and AFC were found to be predictors of the adequate ovulatory response to CC . - pubmed
Protocorm - like body ( PLB ) segments formed calli in Vacin and Went medium with sucrose . - pubmed
Paperless ophthalmology units are feasible in today 's NHS and can produce near complete cataract data sets ; this can ultimately lead to more comprehensive and reliable aggregate cataract outcome data . - pubmed
For the evaluation of osteogenic differentiation , alkaline phosphatase ( ALP ) activity and staining , Alizarin red staining , and a quantitative real - time polymerase chain reaction ( RT - PCR ) analysis were performed . - pubmed
The increased level of ALP production , deposition of calcium mineralization , as well as the expression of typical osteogenic genes , indicated the enhancement of osteogenic differentiation of hMSCs . - pubmed
Moreover , the incorporation of Au could activate the Wnt / beta - catenin signaling pathway , which seemed to be the molecular mechanism underlying the osteoinductive capability of HA - Au nanoparticles . - pubmed
Furthermore , TAZ regulates the expression of Epithelial Membrane Protein 1 ( EMP1 ) , which , in turn , induces the expression of nicotinamide adenine dinucleotide phosphate ( NADPH ) Oxidase 4 ( NOX4 ) , a renal - enriched reactive oxygen species ( ROS)-generating enzyme essential for ferroptosis . - pubmed
ISR / ISO was associated with age ( p = 0.01 ) and CRP level > 5 mg / l ( p = 0.043 ) , while greater stent length was associated with ISR only in the DES group ( p = 0.024 ) . - pubmed
Herein , we report a new multifunctional supramolecular eutectic liquid ( SEL ) binder , consisting of diphenylamine ( DP ) and benzophenone ( BP ) , which enables a strongly improved conductive network and anchored polysulfides for high - performance lithium - sulfur batteries . - pubmed
Airway inflammation ( assessed by exhaled nitric oxide ( eNO ) ) increases after a single high - fat meal ( HFM ) , yet this response may be modified by airway stretch and baseline eNO level . - pubmed
The results showed that after WAS was treated by 20 mg / g total suspended solids ( TSS ) Fe ( II ) and 50 mg / g TSS SPC , the water content of sludge cake ( WCSC ) by press filtration and capillary suction time ( CST ) dropped from 90.8 % + - 1.6 % and 96.1 + - 4.0 s ( the control ) to 55.6 % + - 1.4 % and 30.1 + - 2.5 s , respectively . - pubmed
Biophysical study of phloretin with human serum albumin in liposomes using spectroscopic methods . - pubmed
In this study , the interaction between phloretin and human serum albumin ( HSA ) in an L - egg lecithin phosphatidylcholine ( PC ) liposome suspension was investigated by fluorescence and absorbance spectroscopy . - pubmed
The results show that phloretin efficiently quenches the intrinsic fluorescence of HSA through a combination of dynamic and static quenching . - pubmed
The values of Gibbs free energy , and the enthalpy and entropic change in the binding process of phloretin with HSA in the PC liposome suspensions were negative , suggesting that the binding process of phloretin and HSA was spontaneous . - pubmed
In addition , binding of phloretin to HSA in liposome suspensions was investigated by synchronous fluorescence spectroscopy . - pubmed
The pooled standard mean difference ( 95 % confidence interval ) between exposed and unexposed groups was estimated as of 0.74 ( 0.28 , 1.21 ) for alkaline phosphatase ( ALP ) , 2.44 ( 1.80 , 3.08 ) for aspartate transaminase ( AST ) , 2.06 ( 1.42 , 2.69 ) for alanine transaminase ( ALT ) , 0.10 ( -0.09 , 0.29 ) for total Bilirubin ( TB ) , 0.74 ( -0.42 , -1.90 ) for total protein ( TP ) , -0.49 ( -0.82 , -0.15 ) for albumin , 0.88 ( -0.10 , 1.87 ) for uric acid , 1.02 ( 0.45 , 1.59 ) for creatinine and 1.44 ( 0.75 , 2.13 ) for blood urea nitrogen ( BUN ) . - pubmed
The metal - ion activator , Mg2 + , did not affect the equilibrium between nucleotide and enzyme . - pubmed
The beginning of this plateau depended on both the albumin preparation and the Polybrene lot . - pubmed
Modulation of glucose metabolic response to endotoxin by granulocyte colony - stimulating factor . - pubmed
The present study examines whether in vivo administration of granulocyte colony - stimulating factor ( G - CSF ) and the resultant neutrophilia alters basal glucose metabolism or modulates the glucose metabolic response to a subsequent endotoxin [ lipopolysaccharide ( LPS ) ] challenge . - pubmed
The elevated rate of whole body glucose utilization was attributable to the G - CSF - enhanced LPS - induced increase in glucose uptake by the ileum , spleen , liver , and lung . - pubmed
While basal enzyme activity was not altered , galanin from 10(-11 ) M to 5 x 10(-7 ) M decreased forskolin- and VIP - stimulated adenylate cyclase with a half - maximal effect being elicited by 0.7 nM neuropeptide and a maximal 80 % inhibition of the enzyme activity . - pubmed
The galanin fragments ( 2 - 29 ) and ( 1 - 15 ) dose - dependently inhibited the forskolin - stimulated adenylate cyclase , while the fragments ( 3 - 29 ) and ( 10 - 29 ) were found inactive . - pubmed
The potency of the relaxation induced by 10(-9 ) M of ET-1 was 16.0 + /- 1.3 % , compared to the response to isoproterenol ( 2 x 10(-5 ) M ) , and the duration of the relaxation was 7.2 + /- 0.7 minutes . - pubmed
[ Fluorometric determination of monoaminoxidase activity with 1 - methyl-4 - phenyl-1,2,3,6 - tetrahydropyridine as substrate ] . - pubmed
We studied thrombin - induced changes in cytosolic calcium concentration ( Ca2 + i ) using microfluorometry of fura-2 - loaded single cells , cell topography ( scanning electron microscopy ) , and cytoskeleton ( rhodamine phalloidin ) in endothelial cells . - pubmed
Thrombin caused an initial and sustained phase of an increase in Ca2 + i . - pubmed
Pretreatment with pertussis toxin abolished both phases of Ca2 +i response . - pubmed
Sustained phase of thrombin effect required extracellular calcium . - pubmed
Pretreatment of endothelial cells with indomethacin protracted the sustained phase , whereas a lipoxygenase inhibitor , nordihydroguaiaretic acid curtailed it . - pubmed
Thrombin caused a marked retraction of confluent endothelial cells coincident with the sustained phase of Ca2 +i response . - pubmed
Pretreatment of endothelial cells with nordihydroguaiaretic acid blunted the thrombin - induced cell retraction . - pubmed
The sustained response required microinjection of phospholipase A2 or co - injection of phospholipase A2 with phosphatidylinositol 4,5 - bisphosphate - specific phospholipase C , phosphatidylinositol 1,4,5 - trisphosphate , or CaCl2 , further implying that thrombin receptor(s ) can be coupled to both phospholipases C and A2 . - pubmed
Sustained elevation of Ca2 + i was a necessary prerequisite for the thrombin - induced changes in endothelial cell topography . - pubmed
The biological activity and heparin binding ability was retained when the serine was substituted for the cysteine residue at either 70 or 88 of the bFGF protein . - pubmed
The substitution of the residues at these positions , especially at position 88 , reduced the heterogeneity recognized as several peaks of bFGF eluted from a heparin affinity column , even after oxidation with hydrogen peroxide , suggesting that the cysteines at these positions are exposed to the surface of the molecule to form disulfide bonds that induce heterologous conformations . - pubmed
In this study the dynamic contrast behavior after Gd - DTPA of different breast tissues and tumors has been investigated with a series of T1 - weighted FLASH - sequences during the first 5 minutes after the application of Gd - DTPA . - pubmed
DD between carcinomas and proliferative dysplasia ) was possible on FLASH - scans early after contrast medium than on the late SE - scans . - pubmed
, dipeptidylpeptidase II ( DPP II ) reaction was carried out in smears of peripheral blood using Lys - Pro - MNA and Lys - Ala - MNA as substrates and Fast Blue B ( FBB ) as the coupling agent in cacodylate buffer , pH 5.5 . - pubmed
Inactivation of sulfatase A by calomel electrodes . - pubmed
Hemoglobin F - Caltech : alpha 2 G gamma 2 120Lys replaced by Gln . - pubmed
In in vitro experiments , the inactivation of rheumatoid factor and the inhibition of collagenase and bone alkaline phosphatase activities were observed with both compounds , but these effects of SA96 were more potent than those of D - PA . - pubmed
Within 10 sec of the addition of TRH ( 1 microM ) , there was a maximal 60 % decrease in PtdIns 4,5P2 and 40 % decrease in PtdIns 4P. - pubmed
This early rise in 1,2 - diacylglycerol levels coincided in time and was equivalent in lipid mass with the decrease in the polyphosphoinositide content , suggesting the involvement of a phospholipase C - type enzyme . - pubmed
1,2 - Diacylglycerol levels subsequently fell toward control values and , after 3 min of treatment with TRH , rose again to levels 50 % above normal . - pubmed
These results suggest that TRH stimulates a cycle of events in which the breakdown of the polyphosphoinositides , PtdIns 4,5P2 and , perhaps , PtdIns 4P by a phospholipase C enzyme could be the initiating event . - pubmed
Reaction of isolated subunits with the dialdehyde derivative of ADP is slow compared to the initial reaction with native enzyme , indicating differences in the effects of ADP on intact enzyme and subunits . - pubmed
Long - term beneficial effects of endralazine , a new arteriolar vasodilator at rest and during exercise capacity in chronic congestive heart failure . - pubmed
Pulsatile secretion of oestradiol was observed at frequencies similar to LH during the periods 4 - 12 h ( 3 heifers ) and 24 - 36 h ( 2 heifers ) after PG , respectively , resulting in higher ( P less than 0.05 ) mean oestradiol concentrations . - pubmed
ASA and even SA blocked the effects of bradykinin on both parameters . - pubmed
While SA had no effect on LTC4 , ASA blocked the effects of LTC4 upon both tracheal pressure and nerve activity . - pubmed
These results suggest that both LTC4 and bradykinin may act through mediators derived from arachidonic acid not only in increasing tracheal pressures but also to increase RAR nerve activity . - pubmed
In addition , using bovine serum albumin or the synthetic polymer Kollidone , inhibition could be prevented as well as abolished . - pubmed
The reaction includes neuraminidase hydrolysis of glycoprotein , cleavage of sialic acid to pyruvate by N - acetyl neuraminic acid ( NANA )- aldolase , oxidation of pyruvate by pyruvate oxidase which produces hydrogen peroxide , and colorimetry of hydrogen peroxide using the peroxidase - p - chlorophenol-4 - amino - antipyrine method . - pubmed
Although the disease continued to progress during interferon treatment , a change to a regime of deoxycoformycin resulted in an excellent response . - pubmed
Fas antigen ( Ag ) was expressed in various thyroid tissues , including normal subjects . - pubmed
Tyrosine at position 26 in the second hypervariable region in the beta 1 domain of the DQB1 gene is common to these two alleles and is not present in any other known DQB1 alleles . - pubmed
One major B cell epitope , located within the region corresponding to amino acid residues 74 - 248 , was perfectly associated with the amino acid sequence FLEDR at positions 67 - 71 in the beta 1 domain of the DRB gene . - pubmed
Two other epitopes , corresponding to 316 - 441 or 658 - 700 , were associated with the serologically defined HLA - DR52 antigen . - pubmed
Pretreatment with indomethacin did not significantly alter the effects of guanylin and STa . - pubmed
The levels of three OLS , namely leucine - enkephalin ( LEK ) , beta - endorphin ( beta - EP ) and dynorphin A1 - 13 ( DynoA1 - 13 ) of 44 cases with neonatal asphyxia were studied by radioimmunoassay . - pubmed
The OLS levels of CSF were also higher in cases with cerebral injury than in those without cerebral injury , while the levels of OLS in plasma had no difference in these two groups . - pubmed
This receptor demonstrated a rank order of potency for muscarinic antagonist binding characteristic for the M3 receptor : with high affinity binding for hexahydrosiladifenidol , Kd : 16 + /- 2 nM ( + /- S.E. - pubmed
Moderate stimulation of contractility by isoprenaline ( 0.01 mumol / L ) partly reversed the negative force - frequency relationship in NYHA class IV and preserved the positive force - frequency relationship in nonfailing myocardium . - pubmed
The addition of ouabain and BDF 9148 together restored completely the force - frequency relationship in NYHA class IV . - pubmed
The resulting H-2Ld / Q10d protein is homologous to the native H-2Ld heavy chain for the three external domains except for an Arg to His substitution at position 260 . - pubmed
This molecule reacts with H-2Ld - specific mAb that identify determinants on the first and second domains as well as with an anti - Q10 carboxyl - terminal peptide antiserum , but is not recognized by a mAb specific for a determinant of H-2Ld third domain . - pubmed
The integrity of antibody reactivity of the first and second domains together with beta 2 - microglobulin association suggest that our molecule may be considered a good soluble counterpart of the native membrane H-2Ld molecule with which to perform functional studies . - pubmed
The enzyme activity of both domains was completely inactivated in the presence of copper or silver ions and partially inactivated by iron or zinc ions . - pubmed
Serum alkaline phosphatase activity and the urinary hydroxyproline / creatinine ratio were quickly and drastically reduced in all three groups . - pubmed
A significant reduction of serum alkaline phosphatases and the hydroxyproline / creatinine ratio was still present six months after the five day therapeutic course , reflecting a sustained activity of tiludronate even after stopping treatment . - pubmed
During the operation , supplemental ( rescue ) medication consisted of fentanyl 25 micrograms IV , bolus injections in all four treatment groups . - pubmed
Therefore , we evaluated effects of SH on plasma nutrient and pancreatic hormone ( insulin , glucagon ) concentrations . - pubmed
Previous procedures were followed except dietary carbohydrate was standardized to glucose polymers for all three diets , and sampling was extended to 3 h. Overall , portal or arterial nutrient and hormone concentrations were not different in the SI and SH groups ( P & gt ; 0.05 ) , indicating that hydrolyzed soy protein did not cause abnormal plasma concentrations . - pubmed
The overall mean ( SD ) urea clearance was 26.6 ( 6.0 ) ml.min-1 and the overall creatinine clearance was 30.1 ( 6.3 ) ml.min-1 . - pubmed
Creatinine clearance was significantly lower in filters that failed within 24 h ( filters & lt ; 24 h 27.5 ( 6.3 ) ml.min-1 ; filters & gt ; 24 h 32.2 ( 5.5 ) ml.min-1 ) . - pubmed
Purified rGal was homogeneous as judged by SDS - PAGE analysis with the relative molecular mass under reducing conditions of 39.8 , and under nonreducing conditions 38.0 kDa . - pubmed
Serum cortisol levels and their diurnal variations , ACTH and 24 - h urinary 17 - ketosteroids , ketogenic steroids , 17 - hydroxy steroids , and creatinine , were measured . - pubmed
Ion - dependent inactivation of barium current through L - type calcium channels . - pubmed
These observations suggest that activation of a novel staurosporine - sensitive protein kinase is necessary for basal expression of the preproAM gene in these cells . - pubmed
The profiles of SF extracts of aqueous homogenates of ramp bulbs from three different seasons and growing regions revealed that the thiosulfinates were major components . - pubmed
It recognizes a carbohydrate antigen ( Ley ) which is expressed in a variety of solid tumors including breast and colon . - pubmed
Pre - incubation of cell cultures with cholera toxin significantly down - regulated lipopolysaccharide - induced NO synthesis and phorbol-12 - myristate-13 - acetate - induced respiratory burst . - pubmed
Analysis of a phenobarbital - responsive enhancer sequence located in the 5 ' flanking region of the chicken CYP2H1 gene : identification and characterization of functional protein - binding sites . - pubmed
We previously identified in the chicken CYP2H1 gene an upstream enhancer domain ( -5900/-1100 ) that responds to phenobarbital . - pubmed
Melittin rapidly quenched the isotropic signal in the EPR spectrum of spin - labeled stearic acid added to SR membranes . - pubmed
It was demonstrated with the use of a cross - linking agent , cupric o - phenanthroline , that melittin induced slow aggregation of Ca - ATPase protein in SR membranes ; the content of enzyme in the monomeric form decreased with rate constant 0.14 min-1 . - pubmed
Furthermore , IGF-1 facilitates glucose metabolism , lowers insulin levels , increases insulin sensitivity , and improves the lipid profile . - pubmed
In the brain the renin - angiotensin system has been implicated in the regulation of the cardiovascular system as well as of regulation of reproductive hormones , water balance and behavior . - pubmed
This biologically active Ang II 3 - 8 fragment is referred to as angiotensin IV ( Ang IV ) and has been reported to participate in memory acquisition and recall as well as in the regulation of blood flow , neurite outgrowth , angiogenesis and kidney function . - pubmed
Its tryptic peptide mass profile was determined using the same technique , and the amino acid sequence of two internal peptides was obtained by tandem MS and Edman degradation . - pubmed
The two enzymes showed the highest affinity for angiotensin III ( Ki & lt ; 1 microM ) and a preference for ahydrophobic residue at the P1 ' site . - pubmed
A highly enantioselective methodology for the synthesis of the GABA ( B ) receptor agonist ( R )-(-)- baclofen is described . - pubmed
Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer . - pubmed
Adding ten per cent beta - cyclodextrin , a cyclic oligosaccharide that binds cholesterol and bile acids to the lithogenic diet at the expense of sucrose , induced a decrease in cholesterol bile secretion and in the CSI and HI and prevented cholesterol gallstone formation . - pubmed
Efficient ( 13 ) C labeling was achieved by blocking the endogenous pathway genetically or chemically during the feed of a ( 13 ) C labeled precursor specific to the MEP or MVA pathways . - pubmed
Liquid chromatography - mass spectrometry analysis demonstrated that the prenyl group of trans - zeatin ( tZ ) and isopentenyladenine is mainly produced through the MEP pathway . - pubmed
In comparison , a large fraction of the prenyl group of cis - zeatin ( cZ ) derivatives was provided by the MVA pathway . - pubmed
Organotin derivatives were measured in both the visceral coil and the rest of the soft body of the organisms . - pubmed
in the visceral coil , and from 96 + /- 24 to 297 + /- 107 ng Sn g(-1 ) d.w . - pubmed
( visceral coil ) and from 0.25 to 32 + /- 22 ng Sn g(-1 ) d.w . - pubmed
Recombinant factor VIIa ( NovoSeven ) [ also known as recombinant activated factor VII or eptacog alfa ] is a vitamin K - dependent glycoprotein that is structurally similar to human plasma - derived factor VIIa . - pubmed
In the BPI group , there were significantly more nulliparous women , with more use of oxytocin . - pubmed
Pharmacokinetics and pharmacodynamics of etizolam are influenced by polymorphic CYP2C19 activity . - pubmed
OBJECTIVE : To examine the effect of cytochrome P450 ( CYP ) 2C19 activity on the single - dose pharmacokinetics and pharmacodynamics of etizolam . - pubmed
CONCLUSION : The present study suggests that the single - dose pharmacokinetics and pharmacodynamics of etizolam are influenced by polymorphic CYP2C19 activity . - pubmed
Data was compared between the 2 acquisition methods at rest and between the single breath - hold acquisition at rest and during adenosine stress . - pubmed
Herein , ab initio density functional theory is used to study the thermal decomposition of the Keggin molecular HPA structure through the desorption of constitutional water molecules . - pubmed
The overall reaction energy and activation barrier are computed for the overall reaction HnXM12O40 - ->Hn-2XM12O39+H2O. and subsequently used to predict the effect of HPA composition on thermal stability . - pubmed
We designed and validated the real - time quantitative polymerase chain reaction ( Q - PCR ) assays with SYBR Green dyes for simultaneous quantification of the expression of the 16 genes . - pubmed
Potassium permanganate footprinting on the nontemplate and template strands indicates that when polymerase is in a stable ( open ) complex with P(minor ) , the DNA is single stranded from positions -11 to +4 . - pubmed
Co - treatment with EGCG and the protein synthesis inhibitor cycloheximide prominently shortened Bad half - life , with as little as 30 % of the Bad protein content remaining after 2 h , suggesting an effect of EGCG on Bad protein degradation . - pubmed
Accordingly , the proteasome inhibitors MG-132 and lactacystin damped Bad down - regulation by EGCG . - pubmed
The general protein kinase C ( PKC ) inhibitor GF109203X , or the down - regulation of conventional and novel PKC isoforms , abolished EGCG - induced Bad decline . - pubmed
The enforced expression of Bad for up to 72 h rendered the cells more susceptible to serum deprivation - induced cell death , whereas EGCG treatment significantly improved cell viability ( up to 1.6 - fold ) . - pubmed
The present study reveals a novel pathway in the neuroprotective mechanism of the action of EGCG , which involves a rapid PKC - mediated degradation of Bad by the proteasome . - pubmed
Functional significance of Asn - linked glycosylation of proteinase 3 for enzymatic activity , processing , targeting , and recognition by anti - neutrophil cytoplasmic antibodies . - pubmed
PR3 has two potential Asn - linked glycosylation sites . - pubmed
Using glycosylation deficient rPR3 mutants we found that glycosylation at Asn-147 , but not at Asn-102 , is critical for thermal stability , and for optimal hydrolytic activity of PR3 . - pubmed
Efficient amino - terminal proteolytic processing of rPR3 is dependent on glycosylation at Asn-102 . - pubmed
AIM : To evaluate and compare the clinical outcomes of prophylactic submucosal saline - epinephrine injection and saline injection alone for large colon polyps by conventional polypectomy . - pubmed
Surface properties of ultrathin films of cellulose esters deposited onto silicon wafers have been investigated by means of contact angle measurements and atomic force microscopy ( AFM ) . - pubmed
Only CAB films with lower degree of esterification presented dewetting , CAB films with high degree of butyrate were stable even after one week annealing . - pubmed
Negative values of A(eff ) were found for CA , CAP , and lower butyrate content CAB , which are related to instability and agree with dewetting phenomena observed by AFM . - pubmed
In contrast , a positive A(eff ) was determined for higher butyrate content CAB , corroborating with experimental observations . - pubmed
The mean intensity in grayscale units ( 0 - 255 ) of anti - gentamicin labeling for type I hair cells was 28.14 ( 95 % CI 24.60 - 31.69 ) , for type II hair cells was 17.09 ( 14.99 - 19.20 ) , and for support cells was 5.35 ( 5.34 - 5.46 ; p & lt ; 0.001 , ANOVA ) . - pubmed
Intensity of fluorescence with Texas - Red conjugated gentamicin was 25.38 ( 22.83 - 27.94 ) in type I hair cells , 15.60 ( 14.73 - 16.48 ) in type II cells , and 12.62 ( 12.06 - 13.17 ) in support cells ( p & lt ; 0.001 , ANOVA ) . - pubmed
In addition , zolpidem ( 100 nm ) , a modulator of alpha(1 ) subunit - containing GABA ( A ) receptors , selectively affected mIPSC(S ) suggesting that different postsynaptic GABA ( A ) receptors mediate mIPSC(F ) and mIPSC(S ) . - pubmed
As CGP55845 , a GABA ( B ) receptor antagonist , eliminated the observed difference in PPR , the lower release probability at IPSC(F ) connections probably reflects a stronger tonic GABA ( B ) receptor - mediated inhibition of IPSC(F ) synapses . - pubmed
Thus , CR cells receive two GABAergic inputs , which differ in the quantal amplitude , the probability of GABA release and the frequency dependence of signal transfer . - pubmed
Calcimimetics drug such as cinacalcet suppress the secretion of parathyroid hormone by sensitizing parathyroid calcium receptors to extracellular ionized calcium . - pubmed
The influence of a new parenteral lipid emulsion ( LE ) containing fish oil ( SMOF ) was experimentally evaluated on neutrophils ' chemotaxis and macrophages ' phagocytosis . - pubmed
Only MCT / LCT / FO increased the number of spleen resident macrophages that had engaged in phagocytosis ( p<0.05 ) . - pubmed
SMOF LE had neutral effect while fish oil LE enriched with MCT / LCT LE increased resident - macrophages ' phagocytosis . - pubmed
Ganglioside GM1 - binding sites in interleukin-4 : a photoaffinity labeling study . - pubmed
Plasma cortisol concentration was increased during hypercapnia , and administration of exogenous cortisol caused a marked increase in NHE3 mRNA and protein . - pubmed
Thus we speculate that the elevation of plasma cortisol during hypercapnia contributes to transcriptional activation of NHE3 that ultimately promotes acid - base regulation by stimulating H (+ ) secretion and HCO ( 3 )(- ) reabsorption . - pubmed
The resveratrol analogue 3,5,3 ' , 4 ' , 5 '- pentahydroxy - trans - stilbene inhibits cell transformation via MEK . - pubmed
Further , we identified MEK / ERK signaling as the direct molecular target for the anticancer effects of RSVL2 and demonstrated that RSVL2 inhibited MEK1 , but not Raf1 or ERK2 kinase activity . - pubmed
RSVL2 also dose - dependently suppressed MEK1 kinase activity induced by TPA and the inhibition of H - Ras - induced cell transformation was much stronger for RSVL2 than for PD098059 or resveratrol . - pubmed
Both in vitro and ex vivo pull - down assays indicated that RSVL2 , but not resveratrol , directly bound with GST - MEK1 , but did not compete with ATP for binding . - pubmed
Fasting blood samples were analyzed for glucose , HbA1 - c , insulin , C - peptide , leptin and lipid profile . - pubmed
RESULTS : Mean serum levels of glucose ( fasting and following 2h - OGTT ) , C - peptide and leptin in male offspring of diabetic parents were higher than in male offspring of NDP . - pubmed
Oxorhenium - mediated cyclization and self - assembly of peptides : a combinatorial approach for the selection of new ligands of the human cyclophilin hCyp-18 . - pubmed
Vasopressin ( VP ) is typically reserved as a second - line agent due to the concern of ischemia . - pubmed
CONCLUSIONS : Results indicate that DB is ineffective in increasing CO or improving mesenteric blood flow when used with physiologic replacement doses of VP . - pubmed
Addition of aspartate increased acid survival of the wild type but not the aspA knockout mutant . - pubmed
AspA increases acid survival by producing ammonia as demonstrated by mutation and in vitro enzyme activity analyses . - pubmed
Calcineurin inhibitors such as cyclosporin A ( CsA ) are the mainstay of immunosuppressive treatment for organ transplant recipients . - pubmed
The proportion of SON to total soluble nitrogen ( TSN ) in 0 - 20 cm soil layer was 79.17 - 80.78 % for CAC , 68.64%-74.51 % for CUL , and 59.97%-69.66 % for ALG . - pubmed
With increasing soil depth , the proportion of soil SON to soil TSN and total nitrogen ( TN ) for the three forest stands all increased . - pubmed
Soil SON content had a significant positive correlation with soil organic carbon ( r=0.982 , P<0.01 ) , and also , had definite correlations with soil TSN , TN , NH4 (+)- N , and NO3 (-)- N. - pubmed
The testes , male accessory glands ( MAGs ) , ovaries , and CA contained the long - chain intermediates in the JH biosynthetic pathway , farnesoic acid ( FA ) , methyl farnesoate ( MF ) , and JH III . - pubmed
The testes and ovaries , but not CA , produced radioactive JH III after the addition of ( 3 ) H - methionine and , separately , unlabeled methionine , to the incubation medium . - pubmed
Addition of FA to incubation medium yielded increased JH III , again indicating methylation of FA to MF in the testes , MAGs , ovaries , but not CA . - pubmed
germari proceeds via oxidation to FA , methylation to MF , and epoxidation to JH III . - pubmed
The results indicated that the frequently of G allele and Ile / Val + Val / Val of GSTP1 gene ( 26.7%and 44 % respectively ) in AL group were higher than those in control group ( 10 % and 16 % respectively ) ; the AL risk for persons with Ile / Val + Val / Val was 3.260 - fold ( 95%CI = 1.527 - 5.236 ) of persons with Ile / Ile . - pubmed
Combined genotype analysis showed that the AML risk for persons in combination of lle / Val + Val / Val of GSTP1 gene with C1C2 + C2C2 of CYP2E1 gene increased by 3.208 - fold . - pubmed
It is concluded that the GSTP1 gene is related with susceptibility to AML , the AL risk for persons with lle / Val + Val / Val of GSTP1 gene decreased , while CYP2E1 gene is not related with susceptibility to AL , the AML risk for persons in combination of GSTP1 wildtype with CYP2E1 hybrid and mutant genotype can be further decreased . - pubmed
Furthermore , estrogen receptor 1 mRNA is over - expressed in early - stage tumors , suggesting that expression of the oviduct - related genes may be regulated by estrogen . - pubmed
Oxidized and nitrated oleic acid in biological systems : analysis by GC - MS / MS and LC - MS / MS , and biological significance . - pubmed
cis - EpOA is formed from oleic acid by the catalytic action of various cytochrome P450 isozymes . - pubmed
The sensitivity and specificity of the MS / MS approach make electron - capture negative ion chemical ionization ( ECNICI ) GC - MS / MS and negative electrospray ionization ( NESI ) LC - MS / MS methodologies indispensable in experimental and clinical settings on oxidative and nitrative oleic acid metabolism . - pubmed
On the basis of the influence of the oxygen content in solution on the photocurrent generation , the enzymatic activity of glucose oxidase is evaluated in solution . - pubmed
We show that the inducing effect of NRB on the PT ( i ) is inhibited by the selective ligands of the 18kDa outer membrane ( OMM ) translocator protein ( TSPO , formerly peripheral benzodiazepine receptor ) protoporphyrin IX , N , N - dihexyl-2 -( 4 - fluorophenyl ) indole-3 - acetamide and 7 - chloro-5 -( 4 - chlorophenyl )- 1,3 - dihydro-1 - methyl-2H-1,4 - benzodiazepin-2 - one ; and ( ii ) is lost in digitonin mitoplasts , which lack an intact OMM . - pubmed
Our results also indicated that SH2 domains coevolved and the number of the domains expanded alongside protein tyrosine kinases and tyrosine phosphatases , thereby coupling phosphotyrosine signaling to downstream signaling networks . - pubmed
Recently , it was demonstrated that a specialized Ca ( 2 +)-activated anion - channel , bestrophin1 ( Best1 ) , found on glial cells , can release GABA that contributes up to 50 - 75 % of the tonic GABAergic current . - pubmed
However , it is unknown if ethanol has any actions on Best1 function . - pubmed
We attempted to measure the Best1 - mediated component of the tonic current in slices using 5 - nitro-2 -( 3 - phenylpropylamino ) benzoic acid ( NPPB ) . - pubmed
To better isolate the Best1 - dependent tonic current component , we blocked the Golgi cell component of the tonic current with tetrodotoxin and found that NPPB similarly and significantly potentiated the tonic current amplitude and decay time of miniature IPSCs . - pubmed
Two other Cl (-)- channel blockers were also tested : 4 '- diisothiocyanatostilbene-2,2 '- disulfonic acid disodium salt hydrate ( DIDS ) showed no effect on GABAergic transmission , while niflumic acid ( NFA ) significantly suppressed the tonic current noise , as well as the mIPSC frequency , amplitude , and area . - pubmed
These data suggest that acute ethanol exposure does not modulate Best1 channels and these findings serve to challenge recent data indicating that these channels participate in the generation of tonic GABAergic currents in cerebellar GCs . - pubmed
Laninamivir octanoate ( LO ) ( Inavir ; Daiichi Sankyo , Japan ) is an ester prodrug of the neuraminidase inhibitor laninamivir . - pubmed
These results demonstrated that the prolonged high retention of laninamivir in the respiratory tissues was attributed to a consecutive series of three steps : uptake of LO into the airway epithelial cells , hydrolysis of LO into laninamivir by intracellular esterase(s ) , and limited efflux of the generated laninamivir due to its poor membrane permeability . - pubmed
ZBTB1 depletion impairs formation of phospho - KAP-1 at UV damage sites and reduces RAD18 recruitment . - pubmed
We propose that ZBTB1 is required for localizing phospho - KAP-1 to chromatin and enhancing RAD18 accessibility . - pubmed
The availability of a large quantity of the powder sample allows us to determine the crystal and magnetic structures by neutron powder diffraction ( NPD ) at 300 and 1.3 K. Measurements of the magnetization and heat capacity showed a ferrimagnetic transition at 160 K. A ferrimagnetic structure consisting of the uncompensated antiferromagnetic coupling between neighboring collinear copper and iron spins has been resolved from the low - temperature NPD data . - pubmed
After infection control measures and prolonged decontamination have been implemented with a high adherence , six patients remained sustained CA - MRSA USA300 carriers , including one who developed mupirocin resistance and six who experienced minor CA - MRSA - related SSTIs . - pubmed
CA - MRSA decontamination using mupirocin and chlorhexidine in the community setting may also be a questionable strategy , associated with failure and resistance to both agents . - pubmed
After nucleotide(s ) are incorporated across from the altered base(s ) , the aberrant primer termini are typically extended by DNA polymerase zeta ( pol zeta ) . - pubmed
Here we used brain penetrant tyrosine kinase inhibitors ( TKIs ) , including nilotinib and bosutinib and showed that they reduce the level of nuclear TDP-43 , abrogate its effects on neuronal loss , and reverse cognitive and motor decline . - pubmed
Nilotinib decreased soluble and insoluble TDP-43 , while bosutinib did not affect the insoluble level . - pubmed
Along with elevation in serum and hippocampal TNF - alpha and corticosterone levels associated with significant increase in hippocampal kynurenine / tryptophan ratio . - pubmed
In particular , the effects of vasoactive intestinal peptide and secretin on intra - acinar cell adenosine 3 ' , 5 '- cyclic monophosphate are explored . - pubmed
Considerable attention is paid to regulation of beta - cell function and includes studies detailing the mechanisms of regulation of insulin by somatostatin , serotonin , and melanocortins . - pubmed
BACKGROUND : Zanamivir is a neuraminidase ( NA ) inhibitor used for the treatment of influenza . - pubmed
The combination of high selectivity of aptamer with the peroxidase - mimicking property of DNAzyme has presented considerable opportunities for designing colorimetric aptasensor for detection of ochratoxin A ( OTA ) . - pubmed
The presence of OTA triggers the opening of the hairpin structure and the beginning of HCR , which results in the release of many DNAzyme , and generates enhanced colorimetric signals , which is correlated to the amounts of OTA with linear range between 0.01 to 0.32 nM , and the limit of detection is 0.01 nM under optimal conditions . - pubmed
Coordinated GnRH1 release , not simply elevated absolute levels , effects the release of pituitary gonadotropins that drive steroid production in the gonads . - pubmed
We report that GnRH1 neurons are strongly and uniformly interconnected by electrical synapses that can drive spiking in connected cells and can be reversibly blocked by meclofenamic acid . - pubmed
Among 46 differential metabolites found in the ultra - performance liquid chromatography quadrupole time of flight mass spectrometry ( UPLC - Q - TOF / MS ) and ( 1 ) H NMR - based urinary metabonomics , 20 were significantly correlated with the preferred sucrose consumption observed in behavior experiments and were considered as biomarkers to evaluate the antidepressant effect of CSGS . - pubmed
While the chemical constituents of Bai - Shao contributed to CSGS were paeoniflorin , albiflorin , isomaltopaeoniflorin , and benzoylpaeoniflorin based on the multivariate analysis of the UPLC - Q - TOF / MS chemical profiles from CSGS and QBS extracts . - pubmed
NO production , cell viability , cytokines profile were evaluated . - pubmed
BRP at 50 mug / ml inhibited NO production by 78 % without affecting cell viability . - pubmed
We have tried to use quartz crystal microbalance ( QCM ) , which is widely used to analyze interaction among macromolecules , to detect proteolytic fragments of TnT during aging of meat . - pubmed
Erratum to : Prescribing pattern and resource utilization of monoamine oxidase - B inhibitors in Parkinson treatment : comparison between rasagiline and selegiline . - pubmed
N changes were noted post supplementation for cortisol or DHEA , but testosterone was found to be significantly reduced in both diary treatments compared to control ( F = 4.27 , p = .022 ) . - pubmed
RESULTS : There was a decreasing linear effect on the intake of DM , organic matter ( OM ) , crude protein ( CP ) , neutral detergent fiber ( NDF ) , total carbohydrates ( TCH ) , and nonfibrous carbohydrates ( NFC ) . - pubmed
The apparent digestibility of DM , OM , CP , NDF , TCH , and NFC , as well as urine nitrogen excretion , nitrogen balance and ruminal parameters , were not influenced by different levels of residual frying oil in the diet . - pubmed
The sterol - regulatory element - binding protein ( SREBP ) pathway may contribute to the underlying pathophysiology of AAP - induced metabolic adverse effects . - pubmed
We explored the association between the variants of the sterol - regulatory element - binding transcription factor-1 ( SREBF1 ) gene and the SREBP cleavage - activation protein ( SCAP ) gene with AAP - induced MetS in a genetic case - control study . - pubmed
CONCLUSIONS : The genetic polymorphisms of SREBF1 could play a role in the mechanism for interindividual variation of AAP - induced MetS. - pubmed
The main enzyme involved in the termination of cGMP effects is phosphodiesterase enzyme 5 ( PDE-5 ) , which is overexpressed in ventricular hypertrophy and heart failure . - pubmed
Keywords used for search included cGMP ( mechanisms and functions ) , EDs ( drugs used ) , nitric oxide , and PDE-5 inhibitors ( clinical applications ) . - pubmed
In addition , we provide evidence that heterogeneous octopamine receptor localization , coupled with local differences in phosphodiesterase activity , underlies the observed differences in cAMP signaling in the axon , cell body , and boutons . - pubmed
Seven of the variables considered explained a proportion of the education - T2DM relationship ( body mass index , alcohol consumption , hypertension , fasting triglycerides , high - density lipoprotein ( HDL ) cholesterol , physical activity and smoking status ) , where the contribution of the variables ranged from 1.0 % to 17.7 % . - pubmed
Exogenous expression of wild - type PNPT1 , but not mutants , rescued ATP production in patient skin fibroblasts , suggesting the pathogenicity of the identified mutations . - pubmed
Eluxadoline ( Viberzi ): A Mu - Opioid Receptor Agonist for the Treatment Of Irritable Bowel Syndrome With Diarrhea . - pubmed
miR-339 - 5p downregulation contributes to Taxol resistance in small - cell lung cancer by targeting alpha1,2 - fucosyltransferase 1 . - pubmed
Spectroscopic investigation of the anticancer alkaloid piperlongumine binding to human serum albumin from the viewpoint of drug delivery . - pubmed
Piperlongumine ( PL ) is a very promising natural agent with a high potential for cancer treatment . - pubmed
Displacement studies indicated that PL and warfarin had separate binding regions in site I. - pubmed
Prediction of outcome using pretreatment 18F - FDG PET / CT and MRI radiomics in locally advanced cervical cancer treated with chemoradiotherapy . - pubmed
PURPOSE : The aim of this study is to determine if radiomics features from 18fluorodeoxyglucose ( FDG ) positron emission tomography / computed tomography ( PET / CT ) and magnetic resonance imaging ( MRI ) images could contribute to prognoses in cervical cancer . - pubmed
We supplement atomistic calculations with thermodynamic arguments to show that codons for ' phase 1 ' amino acids ( those appearing early in evolution ) are more likely than a lower entropy GC - rich sequence to form triplets under tension . - pubmed
The objective of this study was to evaluate ADMI color removal from a biologically treated textile mill effluent by heterogeneous photocatalysis with UV - visible irradiation ( UV - vis ) using a novel catalyst composed of TiO2 supported on hydrotalcite and doped with iron oxide ( HT / Fe / TiO2 ) . - pubmed
Photocatalytic treatments were carried out with TiO2 and HT / Fe / TiO2 of Fe : Ti molar ratios of 0.25 , 0.5 , 0.75 and 1 , with varying catalyst doses ( 0 - 3 mg / L ) , initial pH values ( 4 - 10 ) and UV - vis times ( 0 - 6 h ) . - pubmed
The most efficient composite was HT / Fe / TiO2 1 at pH 10 , also at a dose of 2 g / L , which provided more complete ADMI color removal , from 303 to 9 ADMI color units ( 96 % ) , than unmodified TiO2 , from 303 to 37 ADMI color units ( 88 % ) , under the same conditions . - pubmed
For this reason , the HT / Fe / TiO2 1 composite performed better at pH 10 , because the higher concentration of hydroxide ions present at higher pH favored hydroxyl radical formation . - pubmed
HT / Fe / TiO2 1 was reused five times and still lowered secondary effluent ADMI color below local discharge limits . - pubmed
Benefits of the HT / Fe / TiO2 1 catalyst compared to TiO2 include its lower bandgap energy ( 2.34 eV vs 3.25 eV ) , higher ADMI color removal and its magnetic nature that facilitated its recovery and would reduce treatment costs . - pubmed
The only medication in this group approved for the treatment of CD is pasireotide , a somatostatin receptor ligand . - pubmed
Mechanism of action and available data on efficacy and safety of cell cycle inhibitor roscovitine , epidermal growth factor receptor inhibitor gefitinib , retinoic acid , and silibinin , a heat shock protein 90 inhibitor are also presented . - pubmed
Additionally , elevated preoperative suPAR but not creatinine levels were a predictor of acute kidney injury ( AKI ) after CRLM resection , correlating with a longer postoperative hospitalization . - pubmed
We further provide a liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) approach that can separate and quantify the four normal DNA bases ( A , T , G , and C ) , as well as the four modified cytosine bases ( 5 - methyl , 5 - hydroxymethyl , 5 - formyl , and 5 - carboxyl ) . - pubmed
These results underpin the contribution of magnesium , TRPM7 and MagT1 to autophagy and osteoblastogenesis . - pubmed
Older age ( P & lt ; 0.01 ) , a longer duration of fever ; total and before admission ( P & lt ; 0.01 ) , more negative nitrite test ( P & lt ; 0.01 ) , higher platelet count ( P = 0.04 ) , increased C - reactive protein ( CRP ) ( P & lt ; 0.01 ) and erythrocyte sedimentation rate ( ESR ) ( P & lt ; 0.01 ) , were identified as initial features of infants finally diagnosed with KD . - pubmed
Mean + - SD I - PSS and quality of life scores ( 15.9 + - 6.5 vs 6.8 + - 5.6 and 3.1 + - 1.4 vs 1.5 + - 1.4 , respectively , each p & lt;0.001 ) were significantly improved after holmium laser enucleation of the prostate compared to preoperative values . - pubmed
FGY-1153 treatment inhibited the expression of TGFbeta and the phosphorylation of SMAD2 in the heart . - pubmed
This long - term cyanobacterial recurrence also highlights the past presence of both the anaC and mcyB genes , involved in anatoxin - a and microcystin biosynthesis , respectively , throughout the core . - pubmed
Here , we demonstrate this polaron decoupling effect on the photochemical dynamics in singlet fission ( SF ) of amorphous rubrene thin films embedded in optical microcavities . - pubmed
Body weight , serum biochemical lipid parameters , and those for liver and kidney functions , serum TC , LDL - C , vLDL - C , HDL - C , TG , atherogenic index ( AI ) , ALT , AFT , ALP , gamma - GT , total protein ( TP ) , serum albumin and globulin ratio ( A / G ) , serum creatinine , blood urea nitrogen ( BUN ) and blood urea , and histopathology of hematoxylin and eosin ( H&E ) stained liver and kidney sections of all aforementioned groups were examined in the treated animals . - pubmed
Our aim was to study whether a three - meal diet ( 3Mdiet ) with a carbohydrate - rich breakfast may upregulate clock gene expression and , as a result , allow dose reduction of insulin , leading to weight loss and better glycemic control compared with an isocaloric six - meal diet ( 6Mdiet ) . - pubmed
Body weight , glycemic control , continuous glucose monitoring ( CGM ) , appetite , and clock gene expression were assessed at baseline , after 2 weeks , and after 12 weeks . - pubmed
Upregulation of clock genes seen in this diet intervention could contribute to the improved glucose metabolism . - pubmed
The bleeding group had values of lower albumin after 3 months of operation ( HR 0.28 , 95 % CI ( 0.11 - 0.73 ) , p = .01 ) , higher bilirubin after 3 months of operation ( total bilirubin : HR 1.18 , 95 % CI ( 1.04 - 1.33 ) , p = .01 ) , ( direct bilirubin : HR 1.21 , 95 % CI ( 1.05 - 1.41 ) , p = .01 ) . - pubmed
Propofol exhibits a tumor - suppressive effect and regulates cell viability , migration and invasion in bladder carcinoma by targeting the microRNA-10b / HOXD10 signaling pathway . - pubmed
An efficient and bio - based alginate pillared hydrotalcite ( SA@LDHs ) was fabricated via calcination - reconstruction manner with sodium alginate ( SA ) and hydrotalcite ( LDHs - C ) , and used as novel flame retardant for polypropylene ( PP ) . - pubmed
Two common strategies to feed the CO2 are pH steady ( PS ) and CO2 steady ( CS ) . - pubmed
The optimum concentration of CO2 was estimated based on the PS cultivation data and fed to culture media using the CS approach with no buffering agent . - pubmed
The biomass productivity , CO2 bio - fixation rate , and rubisco activity under CSBPS strategy were 127 , 121 , and 65 % higher than 0.04 % CS strategy , respectively . - pubmed
acetyl - CoA carboxylase ( accD ) and ribulose bisphosphate carboxylase large chain ( rbcL ) ) was evaluated using real - time PCR under different initial iron feeds . - pubmed
In 90 microM initial iron concentration , the expression of accD and rbcL genes showed a 4.8- and 35 - fold increase , respectively , compared to that of the control genes . - pubmed
Finally , in this paper , different mechanisms where iron affects acetyl - CoA carboxylase ( ACCase ) and 1,5 - ribulose bisphosphate carboxylase / oxygenase ( RuBisCo ) are illustrated . - pubmed
Gallic acid ( GA ) is a natural polyphenol , which exhibits a broad spectrum of biological activities , but its therapeutic application was limited due to poor bioavailability and toxicity . - pubmed
GA was chemically conjugated with 4.0 G PAMAM dendrimer at outer surface and characterized by different biophysical methods . - pubmed
In conclusion , findings of this study revealed that PAMAM - GA conjugate improves the bioavailability of GA and specificity towards cancer cellsto manifests apoptotic cell death . - pubmed
The findings of the AMG model suggest that a 1 % increase in energy price leads to a 0.02 % decrease in carbon emission . - pubmed
Evaluation of the Effects of Nobiletin on Toll - Like Receptor 3 Signaling Pathways in Prostate Cancer In Vitro . - pubmed
Therefore , we aimed to evaluate the effects of NOB on the activation of TLR3 signaling pathways in PCa In Vitro . - pubmed
However , the level of TRIF protein and CASP8 decreased after both NOB and Poly I : C incubation . - pubmed
SLC39A1 is a zinc ion transport protein which inhibits the progression of prostate cancer . - pubmed
Log - rank tests for univariate analysis ( gender , age , ethnicity and deprivation ) effects on serum creatinine testing at dabigatran etexilate treatment initiation and 12 - months post initiation were performed . - pubmed
The associations among organophosphate pesticide exposure , oxidative stress , and genetic polymorphisms of paraoxonases in children with attention deficit / hyperactivity disorder . - pubmed
This study will help to clarify the relationship between organophosphate pesticides ( OPs ) and attention deficit / hyperactivity disorder ( ADHD ) related to oxidative stress and paraoxonases ( PON ) polymorphisms to further characterize the gene - environment interaction . - pubmed
Oxidative stress biomarkers , such as 8 - hydroxy-2 - deoxyguanosine ( 8 - OHdG ) , 8 - nitroguanine ( 8 - NO2 - Gua ) , 8 - iso - prostaglandin F2alpha ( 8 - iso - PGF2alpha ) , and 4 - hydroxy-2 - nonenoic acid - mercapturic acid ( HNE - MA ) , were analyzed by using liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) . - pubmed
The estimated AP value was 0.66 ( 95 % CI : 0.17 - 1.15 ) , indicating that 66 % of ADHD cases in DMP - exposed children with the PON1 CT / TT ( rs705381 ) genotype were due to gene - environment interactions . - pubmed
Affinity labeling of gamma - glutamyl transpeptidase of tumor cell AH-130 and transport activity of glutathione and amino acids . - pubmed
A complex of immunoglobulin A ( IgA ) with peroxidase was obtained as a result of interaction of IgA with the enzyme , modified with sodium periodate . - pubmed
The effect of two anticholinergic drugs , Ipratropium bromide , nonselective muscarinic receptor antagonist and Pirenzepine , a selective M1 receptor blocker on bronchospasm induced by metacholine and histamine was investigated . - pubmed
It was shown that both drugs protect significantly metacholine induced bronchospasm , but Ipratropium was more effective than Pirenzepine , which suggest that blockade of the M1 receptors only is not sufficient for a complete limitation of bronchi response to metacholine challenge . - pubmed
Cellular cAMP levels peaked after two h of FSK treatment and remained elevated for 48 h. Chromogranin B mRNA increased with FSK treatment , reaching a maximum of 7 - fold above control after 24 h , while the level of SgII mRNA decreased to a level of 65 + /- 10 % of control after 48 h. The effects of FSK on CgB mRNA appear to be mediated by cAMP , as 8 - bromo - cAMP ( 500 microM ) resulted in a 2.8 - fold increase in CgB mRNA , and H-89 ( 30 microM ) , a selective inhibitor of cAMP - dependent protein kinase , reduced the FSK - mediated response . - pubmed
The intensity of SgII staining was diminished by FSK treatment , most likely a result of a decreased rate of synthesis as well as an increase in the release of SgII . - pubmed
Puromycin applied 15 min before and during dexamethasone treatment blocked the induction of this mRNA , suggesting that additional glucocorticoid induced transcription factor proteins may be required for enhanced calmodulin gene transcription . - pubmed
A two - fold increase in the intensity of an approximately 18 K immunoreactive calmodulin protein band was detected by immunoblotting at 90 min after dexamethasone administration . - pubmed
Corticotropin releasing factor , added for 30 min at the start of steroid treatment , prevented the increase of calmodulin mRNA , as well as the suppression of corticotropin releasing factor - evoked ACTH release caused by dexamethasone . - pubmed
Effects of staurosporine , K 252a and other structurally related protein kinase inhibitors on shape and locomotion of Walker carcinosarcoma cells . - pubmed
The structure / activity relationship of the protein kinase inhibitors , staurosporine and K 252a and their analogues on motility of Walker carcinosarcoma cells has been studied in vitro . - pubmed
CGP 41251 , a staurosporine derivative with a much higher specificity for protein kinase C ( PKC ) than staurosporine , induces a dose - dependent increase in the proportion of polarised cells , and stimulates cell locomotion . - pubmed
Two K252a analogues , KT 5720 and KT 5822 , which act preferentially on cyclic nucleotide - dependent protein kinases , and CGP 42700 , an inactive staurosporine analogue , had no effect on cell polarity and locomotion . - pubmed
The blocking activity of the eluted MP+ HS - PG bands depended partly on the amount of GAG attached to the protein core and presumably partly on the structure of the core itself . - pubmed
Carboxypeptidase E ( CPE ) catalyses the removal of C - terminal basic amino acids and is implicated in the processing of peptides and hormones in secretory vesicles . - pubmed
Fractionation of the intracellular forms of CPE with Triton X-114 at various pH values indicates that the membrane - bound , but not the soluble , form is amphipathic ; this difference probably arises from post - translational modification of the enzyme . - pubmed
Thymocyte subsets have been well characterized on the basis of CD4 and CD8 antigen expression . - pubmed
Using three - colour immunofluorescence , we show that ( i ) while most CD45RA+ cells are present amongst the CD4 - CD8- thymocyte subset in the normal thymus , after cortisone treatment or irradiation , all four thymocyte subsets co - express significant amounts of CD45RA . - pubmed
Only IL-1 beta-(208 - 240 ) enhanced non - rapid - eye - movement sleep and body temperature , although both IL-1 beta-(208 - 240 ) and IL-1 alpha-(223 - 250 ) stimulated PGE2 production ; both of these peptides failed to stimulate T - cell production . - pubmed
Biochemically , the most diagnostic alteration is a marked reduction in blood copper and ceruloplasmin levels . - pubmed
Physiological coagulation inhibitors ( protein S , protein C and antithrombin III ) in severe preeclamptic states and in users of oral contraceptives . - pubmed
The clinical utility of the carbonic anhydrase ( CA ) inhibitor acetazolamide ( ACTZ ) is limited because of rapid development of tolerance to its effects . - pubmed
The percent anergic infants decreased and serum IgA increased significantly only in Zn - supplemented group . - pubmed
Receptor concentration was determined by measuring binding of the muscarinic antagonist 1 - quinuclidinyl [ phenyl-4 - 3H ] benzilate ( 3H - QNB ) . - pubmed
These observations suggested that measurement of 3H - QNB binding would provide a reliable estimate of muscarinic receptor number on cultured sympathetic neurons . - pubmed
In contrast to the carbachol - induced down - regulation , the reduction in binding after RFCM treatment was not prevented by atropine , indicating that the effect was not mediated by acetylcholine stimulation of muscarinic receptors . - pubmed
For this purpose , the glycans of the protein were liberated from the polypeptide chain by hydrazinolysis . - pubmed
The chemical structures of two minor oligosaccharide units were determined with the aid of exoglycosidases , and by methylation analysis and Smith degradation . - pubmed
[ Regulation of the renin - angiotensin system in congestive heart failure : indications for a total body sodium - dependent regulation of the renin secretion ] . - pubmed
The thrombi were treated with plasmin or sodium chloride and the radioactivity released in the supernatant by the thrombus was determined . - pubmed
Immunoprecipitates containing SM P180 and SM P120 exhibited a protein kinase activity capable of phosphorylating tyrosine residues of both viral gene products but not immune immunoglobulin G molecules . - pubmed
By independently immunoprecipitating each of the two SM - FeSV proteins we found that most of the tyrosine - specific phosphorylating activity was associated with the SM P120 molecule . - pubmed
In vivo analysis of 32P - labeled SM P180 and SM P120 revealed their phosphoprotein nature ; however , both molecules exhibited low levels of phosphorylation and did not contain phosphotyrosine residues . - pubmed
Other hormones , including somatomedins , epidermal growth hormone , sex steroids , and vitamin D metabolites , are also involved in the control of growth . - pubmed
Sodium selenite inhibited AHH activity to a maximum of approximately 70 % . - pubmed
Sodium thiosulphate also inhibited AHH activity by approximately 47 % . - pubmed
Measurements from the triceps surae were made at rest , and before and after prolonged exercise ( uphill walking ) at approximately 75 % VO2 max in low muscle glycogen ( low CHO ) and high muscle glycogen ( high CHO ) conditions . - pubmed
One hundred unrelated patients with type IIa hyperlipoproteinemia have been investigated for their HLA - A and B antigens and compared to 171 normal controls . - pubmed
Release of gastric inhibitory polypeptide ( GIP ) during calcium infusion and in hyperparathyroidism . - pubmed
This caused GIP to respond significantly more to glucose ingestion . - pubmed
The potentiated response of insulin to glucose may be caused , in part , by GIP . - pubmed
Compounds such as insulin were added to the cells upon release from the block to sensitize the cells to the carcinogen that was added during S. Growth of the transformed cells as distinct from nontransformed cells was promoted by growth in medium supplemented with 8X nonessential amino acids . - pubmed
One possible target for chemotherapy is the biosynthesis of the glycosyl phosphatidyl - inositol ( GPI ) of this parasite 's variant surface glycoprotein ( VSG ) . - pubmed
Their incorporation into VSG correlated roughly with toxicity , although they also were incorporated into phospholipids and other proteins . - pubmed
Myristate analogs are useful for studying the mechanism of GPI myristolyation , and they are candidates for antitrypanosomal chemotherapy . - pubmed
Changes in sex hormone binding globulin , total and free testosterone , dihydrotestosterone and delta 4 - androstenedione were found to be independent of the epileptic syndrome type . - pubmed
Among antiepileptic drugs , PHT is associated with higher sex hormone binding globulin and estradiol and lower free testosterone and dihydrotestosterone levels . - pubmed
PB and CBZ , but not PHT , blunt the LH response to exogenous LHRH in PE . - pubmed
The present study is a more extensive examination of sex differences in immunoreactive inhibin and steroidal hormones in term umbilical cord blood . - pubmed
The concentration of dehydroepiandrosterone sulphate ( DHEAS ) and the sex hormone - binding globulin ( SHBG ) capacity were similar in males and females . - pubmed
Inhibin did not correlate with DHEAS either in their arterial serum concentrations or in the arteriovenous concentration differences . - pubmed
Plasma glucose was analysed by a glucose oxidase / oxygen electrode methodology . - pubmed
Sequence comparisons between parental and mutant HN proteins revealed multiple non - adjacent amino acid substitutions in the majority of MAR mutants . - pubmed
L1 is an axonal cell adhesion molecule found primarily on projection axons of both the CNS and PNS . - pubmed
CKII was able to phosphorylate a peptide encompassing amino acids ( aa ) 1,173 - 1,185 , as well as a related peptide representing an alternatively spliced nonneuronal L1 isoform that lacks aa 1,177 - 1,180 . - pubmed
Serine to alanine substitutions in these peptides indicate that the CKII phosphorylation site is at Ser1,181 . - pubmed
Instead , in this study , an increase in anastomotic prostacyclin production over unsutured controls ( control 1093 + /- 222 pg / ml of 6 - keto prostaglandin F ( PGF ) 1 - alpha , n=21 ; anastomosis 2494 + /- 414 , n=21 , mean + /- 1 SEM , p=0.005 ) is demonstrated . - pubmed
MAIN OUTCOME MEASURES : Glucose , cortisol , androgens , estrogens , thyroid hormones , and insulin growth - like factor ( IGF - I ) were measured in basal samples . - pubmed
The GH responses to GH - RH alone , pyridostigmine alone , or the combination were lower in the older than in the younger group and were correlated negatively with age . - pubmed
In contrast , either arginine alone or GH - RH plus arginine produced similar GH responses in the two groups . - pubmed
Adrenergic and purinergic receptors - mediated calcium responses in brown adipocytes . - pubmed
L - Arginine alone failed to affect basal or stimulated ( CCK or yolk ) emptying of the GB , but when combined with L - NMMA , it reversed the enhancement of GB contraction caused by L - NMMA . - pubmed
The basal plasma CCK level was 1.2 + /- 0.3 pmol / l and rose to 7.9 + /- 2.1 pmol / l with CCK8 infusion and to 4.7 + /- 1.8 pmol / l after yolk meal . - pubmed
No significant change in plasma CCK levels was observed in tests with L - NMMA and/or L - arginine . - pubmed
We conclude that under physiological conditions , the GB contractile activity is regulated predominantly by CCK , but endogenous nitric oxide has a tonic relaxing influence on this activity . - pubmed
No differences in either hyaluronate or collagen were found in either strain and 14 days of development and dexamethasone treatment decreased these compounds in both strains . - pubmed
This machinery consists of 3 ' to 5 ' mRNA decay activities that are Mg2 + -dependent , require neither exogenous ATP / GTP nor an ATP - regenerating system , and act independently of a 7mG(5')ppp(5')G cap structure to deadenylate an exogenous mRNA substrate in a c - myc 3'-UTR - dependent fashion . - pubmed
Replacing Val with Pro at P2 in this sequence greatly improved the rate of cleavage by cruzipain . - pubmed
Tyr and Val residues are preferred at P3 by all cysteine proteinases whatever their origin , whereas only cruzipain and cathepsin L cleaved substrate with a His at that position . - pubmed
The combination of a Pro at P2 and His at P3 abolished cleavage by cathepsin L , so that only cruzipain was able to cleave the HPGGP peptide at the GG bond . - pubmed
A substrate with intramolecularly quenched fluorescence was raised on this sequence ( Abz - HPGGPQ - EDDnp ) which was also specifically cleaved by cruzipain ( kcat / Km of 157 000 m-1 . - pubmed
The pH activity profile of cruzipain on Abz - HPGGPQ - EDDnp showed a narrow peak with a maximum at pH 5.5 and no cleavage above pH 6.8 , although trypanosomal cysteine proteinases remain active at basic pH. - pubmed
The importance of the His residue at P3 for cleavage by cruzipain was confirmed by substituting Lys for His at that position . - pubmed
It was concluded that the presence of an imidazolium group at P3 was essential to endow the HPGGPQ sequence with the properties of a cruzipain substrate . - pubmed
Prostate - specific antigen ( PSA ) promoter - driven androgen - inducible expression of sodium iodide symporter in prostate cancer cell lines . - pubmed
Causing prostate cancer cells to express functionally active sodium iodide symporter ( NIS ) would enable those cells to concentrate iodide from plasma and might offer the ability to treat prostate cancer with radioiodine . - pubmed
The PSA promoter is responsible for androgen - dependent expression of PSA in benign and malignant prostate cells and was therefore used to mediate androgen - dependent prostate - specific expression of NIS . - pubmed
Prostate cells transfected with NIS / PSA - pEGFP-1 showed perchlorate - sensitive , androgen - dependent iodide uptake in a range comparable to that observed in control cell lines transfected with hNIS cDNA . - pubmed
Perchlorate - sensitive iodide uptake was not observed in cells transfected with NIS / PSA - pEGFP-1 and treated without androgen or in cells transfected with the control vectors . - pubmed
In conclusion , tissue - specific androgen - dependent iodide uptake activity has been induced in prostate cancer cells by PSA promoter - directed NIS expression . - pubmed
Androgen and estrogen signaling at the cell membrane via G - proteins and cyclic adenosine monophosphate . - pubmed
The system consists of three components , an agonist steroid , sex hormone - binding globulin ( SHBG ) , and a membrane receptor ( R(SHBG ) ) for SHBG . - pubmed
SHBG is a well - characterized plasma protein that has two binding sites , one binds certain estrogens and androgens , and the other binds to R(SHBG ) . - pubmed
The characteristics of this novel signal transduction system , from the interaction of SHBG with R(SHBG ) , to the intermediacy of G - proteins , to cAMP generation , to downstream effects of the second messenger will be reviewed . - pubmed
When cells were incubated in 25 mM glucose to mimic the diabetic state , IL-1beta induction was inhibited in all cases , but to a significantly lesser extent with the 14 - repeat allele . - pubmed
[ Intrinsic nucleoside diphosphate kinase - type activity is a novel function of the molecular chaperone and protease ] . - pubmed
We examined the function of the V - ATPase in cytosolic Ca ( 2 + ) homeostasis by comparing responses to a brief Ca ( 2 + ) challenge of a V - ATPase mutant ( vma2Delta ) and wild - type cells treated with the V - ATPase inhibitor concanamycin A. - pubmed
The kinetics of the Ca ( 2 + ) response were determined using transgenic aequorin as an in vivo cytosolic Ca ( 2 + ) reporter system . - pubmed
In wild - type cells , the V - ATPase - driven Vcx1p was chiefly responsible for restoring cytosolic Ca ( 2 + ) concentrations after a brief pulse . - pubmed
In cells lacking V - ATPase activity , brief exposure to elevated Ca ( 2 + ) compromised viability , even when there was little change in the final cytosolic Ca ( 2 + ) concentration . - pubmed
vma2Delta cells were more efficient at restoring cytosolic [ Ca ( 2 + ) ] after a pulse than concanamycin - treated wild - type cells , suggesting long term loss of V - ATPase triggers compensatory mechanisms . - pubmed
The objective of this study was to determine the impact of endoluminal LPRLL on vascular levels of inducible nitric oxide synthase ( iNOS ) and cyclic guanosine monophosphate ( cGMP ) to help define the mechanism of this effect . - pubmed
For five members of the family of the small leucine - rich proteoglycans ( SLRPs ) , the expression pattern during fetal development was analyzed . - pubmed
To investigate the biochemical mechanism of the effect of chronic insulin delivery by either route on hepatic glucose metabolism , we measured the hepatic activity of glucose 6 - phosphatase ( G6Pase ) and glucokinase ( GK ) . - pubmed
Glucose levels , higher in D , were normalized by insulin treatment regardless of route . - pubmed
STZ diabetes resulted in a 10 - fold decrease in GK ( P & lt ; .001 ) , and a 2 - fold increase in G6Pase activity ( P & lt ; .01 ) . - pubmed
Our current studies indicate that chronic intraperitoneal insulin treatment is superior to subcutaneous treatment with regard to hepatic glucose metabolism . - pubmed
Oxidant / antioxidant parameters , namely superoxide dismutase ( SOD ) , glutathione peroxidase ( GSH - Px ) , catalase ( CAT ) , malondialdehyde ( MDA ) , nonenzymatic superoxide scavenger activity ( NSSA ) , susceptibility to oxidation ( SO ) and antioxidant potential ( AOP ) values , were assayed in the samples . - pubmed
The major lipoprotein fractions ( VLDL , LDL , HDL ) were separated by preparative ultracentrifugation , and TG and cholesterol concentrations were determined in plasma and lipoprotein fractions . - pubmed
In the initial 3 - mon period , total cholesterol remained steady , whereas LDL - cholesterol and total apolipoprotein B showed a significant increase . - pubmed
No significant changes were found in total and transported TG and cholesterol between the 3 - mon and the 6 - yr values . - pubmed
Post - transplant immunosuppression consisted of tacrolimus , mycophenolate mofetil , and prednisolone . - pubmed
BMD of the lumbar spine and femoral neck decreased significantly in the steroid+ group ( -1.4+/-3.2 % and -2.3+/-2.9 % , respectively , P<0.05 ) while no changes were observed in the steroid- group . - pubmed
The change in BMD of the lumbar spine was significantly different between the steroid+ and the steroid- group , whereas the change in BMD of the femoral neck was not significantly different . - pubmed
The central section was stained for tartrate resistant acid phosphatase ( TRAP ) in order to identify osteoclasts , one adjacent section was immunostained with an affinity purified antibody to a 15 amino acid sequence unique to rat PTH / PTHrP receptor , and the other adjacent section in the set served as an immunostaining control . - pubmed
In contrast to AtlE , which is known to influence primary attachment , delta - toxin appeared to exert its effect on attachment to polystyrene during later stages of biofilm formation . - pubmed
Very low doses of ethanol induce behavioral changes involving dopamine D2 receptors . - pubmed
These effects were inhibited by peripheral administration of the dopamine D2 receptor antagonist , sulpiride ( 1 mg / kg ) . - pubmed
The pretreatment with the dopamine D2 receptor agonist , ( + /-)- 2 -( N - phenethyl - N - propylamino )- 5 - hydroxytetralin ( PPHT , 0.1 mg / kg ) , enhanced the effects of ethanol very low doses in delaying the initiation and reducing the duration of narcosis induced by an acute i.p . - pubmed
Thus , repeated administration of ethanol very low doses may have affected the sensitivity of presynaptic dopamine D2 receptors . - pubmed
The 68 and 72 kDa molecular forms of inner ear CTL2 are distinguished by sialic acid modification of the carbohydrate . - pubmed
The KHRI-3 antibody binds to an N - linked carbohydrate on CTL2 and presumably damages the organ of Corti by blocking the transporter function of this molecule . - pubmed
The primary end point was HbA ( 1c ) after 12 weeks ; secondary end points were fasting serum glucose , fasting C - peptide , fasting glucagon , fasting insulin , beta - cell function , body weight , adverse events , and hypoglycemic episodes . - pubmed
HbA(1c ) decreased in all but the lowest liraglutide dosage group . - pubmed
In the 0.75 - mg liraglutide group , HbA(1c ) decreased by 0.75 percentage points ( P & lt ; 0.0001 ) and fasting glucose decreased by 1.8 mmol / l ( P = 0.0003 ) compared with placebo . - pubmed
The proinsulin - to - insulin ratio decreased in the 0.75 - mg liraglutide group ( -0.18 ; P = 0.0244 ) compared with placebo . - pubmed
Reduction of dioxygen catalyzed by pyrene - wired heme domain cytochrome P450 BM3 electrodes . - pubmed
We have electronically wired the cytochrome P450 BM3 heme domain to a graphite electrode with the use of a pyrene - terminated tether . - pubmed
AFM images clearly reveal that pyrene - wired enzyme molecules are adsorbed to the electrode surface . - pubmed
Routine database analyses suggested that two enzymes essential for fatty acid synthesis were missing , one of these is the holo - acyl - carrier - protein synthase . - pubmed
Besides lecithin , surfactant contains a variety of minor lipids , such as plasmalogens , polyunsaturated fatty acid - containing phospholipids ( PUFA - PL ) , and cholesterol . - pubmed
Lipid composition exhibited strong differences : Survanta had the highest proportion of disaturated PL and total neutral lipids and the lowest proportion of PUFA - PL . - pubmed
In Survanta samples , viscosity increased & gt;8 x 10(-6 ) kg / s at surface tension of 30 mN / m. Curosurf showed only slightly increased surface viscosity below surface tensions of 25 mN / m , and viscosity did not reach 5 x 10(-6 ) kg / s. By adding defined PL to Survanta , we obtained a Curosurf - like lipid mixture ( without plasmalogens ) that exhibited biophysical properties like Curosurf . - pubmed
For DNA microarrays , oligonucleotide probes for these targeted genes were deposited onto epoxysilane - derivatized , 12 - well , Teflon - masked slides by using a MicroGrid II arrayer . - pubmed
Direct titanium ( 14 ) and stainless steel ( 14 ) posts were not used extensively . - pubmed
CONCLUSIONS : The majority of the practitioners surveyed in this study used : amalgam for core build - ups ; indirect posts ; addition - cured silicone for impressions ; and glass - ionomer cements for luting procedures . - pubmed
Production of extracellular phospholipase and protease was tested by the agar plate methods . - pubmed
Cerebral emboli may be composed of atherosclerotic debris , calcium , air , fat , platelet thrombi , or CPB tubing . - pubmed
The GGDEF and EAL domain proteins involved in c - di - GMP synthesis and degradation , respectively , are ( almost ) ubiquitous in bacterial genomes . - pubmed
While the role of GGDEF and EAL domain proteins in bacterial physiology and behaviour has gained appreciation , and significant progress has been achieved in understanding the enzymology of c - di - GMP turnover , many questions regarding c - di - GMP - dependent signalling remain unanswered . - pubmed
The rational for this study was to determine if there is an altered risk for liver damage by APAP in the absence of Mrp2 . - pubmed
Mrp2 expression not only is important in biliary excretion , but also influences the toxic potential of reactive intermediates by controlling intrahepatic GSH and possibly drug metabolism . - pubmed
The biological activity of DnaK , the bacterial representative of the Hsp70 protein family , is regulated by the allosteric interaction between its nucleotide and peptide substrate binding domains . - pubmed
Despite the importance of the nucleotide - induced cycling of DnaK between substrate - accepting and releasing states , the heterotropic allosteric mechanism remains as yet undefined . - pubmed
To further characterize this mechanism , the nucleotide - induced absorbance changes in the vibrational spectrum of wild - type DnaK was characterized . - pubmed
To assign the conformation sensitive absorption bands , two deletion mutants ( one lacking the C - terminal alpha - helical subdomain and another comprising only the N - terminal ATPase domain ) , and a single - point DnaK mutant ( T199A ) with strongly reduced ATPase activity , were investigated by time - resolved infrared difference spectroscopy combined with the use of caged - nucleotides . - pubmed
The results indicate that ( 1 ) ATP , but not ADP , binding promotes a conformational change in both subdomains of the peptide binding domain that can be individually resolved ; ( 2 ) these conformational changes are kinetically coupled , most likely to ensure a decrease in the affinity of DnaK for peptide substrates and a concomitant displacement of the lid away from the peptide binding site that would promote efficient diffusion of the released peptide to the medium ; and ( 3 ) the alpha - helical subdomain contributes to stabilize the interdomain interface against the thermal challenge and allows bidirectional transmission of the allosteric signal between the ATPase and substrate binding domains at stress temperatures ( 42 degrees C ) . - pubmed
Diffusion of water in montmorillonite clays at low hydration has been studied on the microscopic scale by two quasi - elastic neutron scattering techniques , neutron spin - echo ( NSE ) and time - of - flight ( TOF ) , and by classical microscopic simulation . - pubmed
In the case of the Na - bihydrated system , the TOF results are in closer agreement with the other two techniques ( the techniques differ by a factor of 2 - 3 at most ) , giving diffusion coefficients of ( 5 - 10 ) x 10(-10 ) m(2 ) s(-1 ) . - pubmed
LC - MS analysis of carbonyl compounds and their occurrence in diesel emissions . - pubmed
The aim of this work was to study the effects of the M , SD and its interaction on 5 - HT and 5 - hydroxy - indole - acetic acid ( 5 - HIAA ) contents in the dorsal raphe ( DR ) and the suprachiasmatic nuclei ( SCN ) , two sleep - wake cycle regulators . - pubmed
DR and SCN were dissected and processed to determine the 5 - HT and 5 - HIAA contents by means of HPLC . - pubmed
We conclude that the sleep - wake cycle disruptions produced by chronic M and SD are mediated in part by a synergistic effect on 5 - HT in the DR - SCN pathway , perhaps due to a delay in the development of such brain structures . - pubmed
An original and novel assay system with urease as a catalyst and CdSe / ZnS quantum dots ( QDs ) as an indicator has been developed for quantitative analysis of urea . - pubmed
The detection is based on the enhancement of QD photoluminescence ( PL ) intensity , which is correlated to the enzymatic degradation of urea . - pubmed
By controlling the buffer concentration and pH , PL enhancement due to the degradation of urea is linear in the urea concentration ranging from 0.01 to 100mM. - pubmed
Here , we conducted ( 19 ) F NMR , CD , T(m ) , and modeling experiments at various primer positions with respect to a template modified by a fluorine tagged AF - adduct ( FAF ) . - pubmed
Molecular modeling indicated that the aminofluorene moiety in the minor groove of the W - conformer would impose a steric clash with the tight - packing amino acid residues on the DNA binding area of the Bacillus fragment ( BF ) , a replicative DNA polymerase . - pubmed
BACKGROUND & amp ; OBJECTIVES : Endo - derived nitric oxide ( NO ) is synthesized from L - arginine by endothelium nitric oxide synthase ( eNOS ) encoded by the NOS3 gene on chromosome7 . - pubmed
Since reduced NO synthesis has been implicated in the development of coronary atherosclerosis ; polymorphisms of NOS gene might be associated with increased susceptibility to this disorder and coronary artery disease ( CAD ) . - pubmed
The interaction and cellular localization of HSP27 and ERbeta are modulated by 17beta - estradiol and HSP27 phosphorylation . - pubmed
Recently , we identified heat shock protein 27 ( HSP27 ) as an estrogen receptor - beta ( ERbeta ) associated protein that acts as a co - repressor of estrogen signaling and serves as a biomarker of atherosclerosis . - pubmed
In vitro we determined that phosphorylated HSP27 is retained in the cytoplasm after treatment with 17beta - estradiol and to a lesser extent with heat shock . - pubmed
HSP27 and ERbeta associate in both the cytoplasm and nucleus , however , co - localization studies reveal that in the presence of 17beta - estradiol , a significant portion of this interaction occurs outside of the nucleus . - pubmed
These data highlight an extranuclear interaction between ERbeta and HSP27 that may be of potential importance in modulating estrogen signaling . - pubmed
Cannabinoid CB1 receptors of the dorsal hippocampus are important for induction of conditioned place preference ( CPP ) but do not change morphine CPP . - pubmed
The cannabinoid CB1 receptor agonist ( WIN55,212 - 2 ) and antagonist ( AM251 ) were injected bilaterally into the dorsal hippocampus . - pubmed
The conditioning treatments with injections of morphine ( 6 and 9 mg / kg ) induced a CPP for the drug - associated place . - pubmed
HIF-1alpha promotes survival of prostate cells at a high zinc environment . - pubmed
METHODS : Immunohistochemistry , histofluorescent zinc staining , Western blot , in vitro binding assay , immunoprecipitation , caspase activity assay , and proteasome activity assay . - pubmed
Moreover , in cultured prostate cells , HIF-1alpha expression was zinc - dependently induced even under normoxic conditions . - pubmed
Mechanistically , zinc ions inhibited HIF-1 - prolyl hydroxylase ( PHD ) activity , and therefore blocked von Hippel - Lindau tumor suppressor protein ( pVHL ) binding to HIF-1alpha in vivo and in vitro . - pubmed
The HIF-1alpha stabilization was mediated by oxidative stress induced by zinc ion . - pubmed
CONCLUSION : These results suggest that HIF-1alpha functions as an intrinsic defense molecule that enables prostate cells to survive in a zinc - rich environment . - pubmed
Drugs targeting serotonin receptors like serotonin reuptake inhibitors ( SSRIs ) are useful in the present therapy of anxiety and depression . - pubmed
The spectroscopic behavior of mono- and dinuclear Ru ( II ) complexes ( P , T , PP and TT , Figure 1 ) that contain the extended planar ligand tetrapyrido [ 3,2 - a : 2 ' , 3 '- c : 3 ' ' , 2 ' ' - h : 2 ' ' ' , 3 ' ' ' - j ] acridine ( TPAC ) and either 1,10 - phenanthroline ( phen ) or 1,4,5,8 - tetraazaphenanthrene ( tap ) as ancillary ligands is examined in water and as a function of the pH. - pubmed
In contrast , with phen ancillary ligands ( P and PP ) , the protonated excited state does not luminesce . - pubmed
Thioglycoside hydrolysis catalyzed by beta - glucosidase . - pubmed
Not surprisingly , in spite of the requirement for the presence of this protonated group in catalytically active beta - glucosidase , thioglucoside hydrolysis does not involve general acid catalysis . - pubmed
There is no solvent kinetic isotope effect on the enzyme - catalyzed hydrolysis of pNPSG . - pubmed
DON had no effect on collagen secretion in RPTEC cells . - pubmed
We also studied the metabolism and the cellular uptake of DON using LC - MS / MS . - pubmed
Apart from known steroid effects , calcitriol in particular promoted regulatory T cell profiles as indicated by a marked increase of IL-10 , TGFbeta , FoxP3 , and CTLA4 . - pubmed
Glucose levels were & gt;11.1 mmol / l throughout 72 h of continuous glucose monitoring , with low insulin secretion during intravenous glucose tolerance testing . - pubmed
Cotransfection of Kir6.2 and mutant SUR1 demonstrate that the mutated protein is expressed efficiently at the cell surface but fails to respond to MgADP , resulting in minimal channel activity . - pubmed
PURPOSE : The aim of the study was to identify variables associated with successful long - term maintenance of sinus rhythm ( SR ) after a single ablation of long - lasting persistent atrial fibrillation ( AF ) . - pubmed
Inosine monophosphate dehydrogenase type II ( IMPDH2 ) is the target for immunosuppression by mycophenolic acid and has been linked to resistance of tumor cells to chemotherapy . - pubmed
The influence of sterols on the conformation of recombinant mitochondrial porin in detergent . - pubmed
When isolated from mitochondria porins copurify with sterols , and some isolated forms of the protein require sterol for insertion into artificial membranes . - pubmed
Nonetheless , the contributions of sterols to the folded state of mitochondrial porin are not understood . - pubmed
Sterols do not significantly alter the secondary structure of porin in lauryl dimethylamine oxide , nor in a mixture of sodium dodecylsulfate and dodecylmaltopyranoside . - pubmed
Serum glucose and insulin were examined in the morning of the 3rd day before milk . - pubmed
Gestational age , birth weight , birth weight percentage , serum glucose , insulin and HOMA - IR were compared among different genotype groups . - pubmed
RESULTS : No significant difference was found between the serum glucose level of SGA group ( 4.03 + /- 1.05 mmol / L ) and AGA group ( 4.05 + /- 1.14 mmol / L ) , P = 0.008 . - pubmed
The dermatitis was accompanied by infiltration of neutrophils and macrophages and by elevated levels of pro - inflammatory cytokines , chemokines and prostaglandin E2 . - pubmed
A series of 3 - acylaminocaprolactams are inhibitors of chemokine - induced chemotaxis . - pubmed
Branching of the side chain alpha - carbon provides highly potent inhibitors of a range of CC and CXC chemokines . - pubmed
The majority of research for the calcitonin gene - related peptide ( CGRP ) in the stomach in the hypertension has been devoted to the submucosal blood flow , and no attention has been paid to its quantitative distribution in the gastric neuroendocrine cells . - pubmed
Moreover , since angiogenesis is essential for follicle development , the effect of the flavonoid on Vascular Endothelial Growth Factor output by granulosa cells was also taken into account . - pubmed
Additionally , the flavonoid interferes with the angiogenic process by inhibiting VEGF production as well as by altering redox status . - pubmed
Chapter 4 : Regulation of Clusterin activity by calcium . - pubmed
In this chapter , the attention is put on Ca ( 2 + ) effect on Clusterin ( CLU ) activity . - pubmed
We showed that two CLU forms ( secreted and nuclear ) are differently regulated by Ca ( 2 + ) and that Ca ( 2 + ) fluxes affect CLU gene expression . - pubmed
Based on available data we suggest , that different CLU forms play opposite roles , depending on intracellular Ca ( 2 + ) concentration , time - course of Ca ( 2 + ) current , intracellular Ca ( 2 + ) compartmentalization , and final Ca ( 2 + ) targets . - pubmed
Discussion will be motivated on how CLU acts on cell in response to Ca ( 2 + ) waves . - pubmed
The impact of Ca ( 2 + ) on CLU gene activity and transcription , posttranscriptional modifications , translation of CLU mRNA , and posttranslational changes as well as biological effects of CLU will be discussed . - pubmed
We will also examine how Ca ( 2 + ) signal and Ca ( 2 +)-dependent proteins are attributable to changes in CLU characteristics . - pubmed
Some elucidation of CLU gene activity , CLU protein formation , maturation , secretion , and intracellular translocations in response to Ca ( 2 + ) is presented . - pubmed
We assume that commonly upregulated mRNA for nCLU versus sCLU and vice versa are dependent on Ca ( 2 + ) accessibility and its intracellular distribution . - pubmed
It looks as if at low intracellular Ca ( 2 + ) the delay in cell cycle allows more time for DNA repair ; otherwise , cells undergo nCLU - dependent apoptosis . - pubmed
In conclusion , a narrow range of intracellular Ca ( 2 + ) concentrations is responsible for the decision whether nCLU is mobilized ( apoptosis ) or sCLU is appointed to improve survival . - pubmed
Nonetheless we feel that much work is left ahead before remaining uncertainties related to Ca ( 2 + ) signal and the respective roles of CLU proteins are unraveled . - pubmed
Negative charge of the glutamic acid 417 residue is crucial for isomerohydrolase activity of RPE65 . - pubmed
RPE65 is the isomerohydrolase essential for regeneration of 11 - cis retinal , the chromophore of visual pigments . - pubmed
Apc ( MIN ) modulation of vitamin D secosteroid growth control . - pubmed
A central paradox of vitamin D biology is that 1alpha,25 -( OH )( 2 ) D ( 3 ) exposure inversely relates to colorectal cancer ( CRC ) risk despite a capacity for activation of both pro- and anti - oncogenic mediators including osteopontin ( OPN)/CD44 and E - cadherin , respectively . - pubmed
Here we investigate effects of the Apc ( Min /+ ) genotype on 1alpha,25 -( OH )( 2 ) D ( 3 ) regulation of OPN / CD44 / E - cadherin signalling and intestinal tumourigenesis , in vivo . - pubmed
Treatment by 1alpha,25 -( OH )( 2 ) D ( 3 ) or rationally designed analogues ( QW or BTW ) enhanced OPN but inhibited expression of CD44 , the OPN receptor implicated in cell growth . - pubmed
All secosteroids suppressed Apc(Min/+)-driven tumourigenesis although QW and BTW had lower calcium - related toxicity . - pubmed
Taken together , these data indicate that the Apc(Min/+ ) genotype modulates vitamin D secosteroid actions to promote functional predominance of E - cadherin tumour suppressor activity within antagonistic molecular networks . - pubmed
APC heterozygosity may promote favourable tissue- or tumour - specific conditions for growth control by vitamin D secosteroid treatment . - pubmed
The absolute quantification method , on the other hand , provides us with an accurate computational tool to calculate absolute values of oxy- , deoxy- , total hemoglobin concentrations , and light scattering coefficients . - pubmed
Laboratory test revealed a hemoglobin of 7.7 g / dl and a bone marrow negative for iron . - pubmed
Up - regulation of the G3PDH ' housekeeping ' gene by estrogen . - pubmed
In our studies implementing estrogen treatments on different cell lines , we initially used glyceraldehyde-3 - phosphate dehydrogenase ( G3PDH ) as an internal standard . - pubmed
However , the results of PCR amplification demonstrated that 17beta - estradiol enhanced the expression of the G3PDH gene , rendering it impossible to use G3PDH as an unbiased comparative control . - pubmed
DNA damage , stalled replication forks , errors in mRNA splicing , and availability of nutrients activate specific phosphatidylinositiol-3 kinase - like kinases ( PIKKs ) that in turn phosphorylate downstream targets such as p53 on serine 15 . - pubmed
Here , we demonstrate that siRNA oligonucleotides targeting the mRNA surveillance proteins SMG1 , Upf1 , Upf2 , or the PIKK protein ATM attenuated p53 ( ser15 ) phosphorylation in cells damaged by high oxygen ( hyperoxia ) , a model of persistent oxidative stress that damages nucleotides . - pubmed
In contrast , loss of SMG1 or ATM , but not Upf1 or Upf2 reduced p53 ( ser15 ) phosphorylation in response to DNA double strand breaks produced by expression of the endonuclease I - PpoI. - pubmed
30 + - 2 days postinfection , jejunal EBF ( electrophysiological parameters , fluorescein isothiocyanate - dextran fluxes and responses to secretagogues and MMC degranulators ) was evaluated ( Ussing chamber ) . - pubmed
spiralis - infected animals , secretory responses to serotonin ( 5 - HT ) and immunoglobulin E ( IgE)-dependent activation of MMCs were reduced . - pubmed
In contrast , responses to substance P ( SP ) and capsaicin were similar in infected and noninfected animals . - pubmed
The altered permeability and the TTX - independent hyporesponsiveness to 5 - HT and IgE indicate epithelial alterations . - pubmed
The mean of the indirect bilirubin measurements for 0- , 1- , and 3 - hour samples were 174 ( SD 68.65 ) , 186.97 ( SD 60.47 ) , and 184.56 ( SD 66.93 ) , respectively . - pubmed
Neuroimaging studies have found moderating effects of dopamine genes during both the anticipation and delivery of rewards , particularly the catechol - O - methyltransferase ( COMT ) genotype . - pubmed
In anticipation of uncertain outcomes , we observed an increased SPN among Met homozygotes . - pubmed
Under low levels of reactive oxygen species ( ROS ) , " normal " amounts of p53 upregulates expression of antioxidant genes , protecting macromolecules from ROS - induced damage . - pubmed
CONCLUSION : The mutation acted in a E - subunit dependent manner , suggesting I(Ks ) function altered by the presence of different KCNE subunits in sinus node , atria and ventricles as the molecular basis of sinus bradycardia , fAF and LQTS in mutation carriers . - pubmed
Then observed the general reaction of each group;The content of cholesterol in the blood and brain tissue was detected ; The neurofibrillary tangles , beta amyloidal protein deposits and loss of midbrain neurons of hippocampus and cortex tissue were observed . - pubmed
In time fluorescence quantitative method was used to detect the hippocampus and cortex tissue metabolism of cholesterol key genes CYP46a1 and APOE expression . - pubmed
Actually the content of cholesterol of group II , III increased obviously , especially group III , while decreases in group IV after the treatment . - pubmed
SIRT3 is a member of the Sir2 family of NAD(+)-dependent protein deacetylases that promotes longevity in many organisms . - pubmed
A vital feature of CaMKII in plasticity is its ability to switch to a calcium ( Ca ( 2 + ) ) independent constitutively active state after autophosphorylation at threonine 287 ( T287 ) . - pubmed
At the cellular level CaMKII overexpression in the alpha'/beta ' neurons increased activity dependent Ca ( 2 + ) responses while reduction of CaMKII decreased it . - pubmed
Likewise reducing CASK or directly expressing a phosphomimetic CaMKII T287D transgene in the alpha'/beta ' similarly decreased Ca ( 2 + ) signaling . - pubmed
Our results are consistent with CASK regulating CaMKII autophosphorylation in a pathway required for memory formation that involves activity dependent changes in Ca ( 2 + ) signaling in the alpha'/beta ' neurons . - pubmed
In a cell - free system , however , ethyl p - methoxycinnamate did not directly inhibit tyrosinase , the rate - limiting enzyme of melanogenesis . - pubmed
These results indicate that the pigment - inhibitory effect of ethyl p - methoxycinnamate results from downregulation of tyrosinase . - pubmed
Atypical zeta ( zeta ) isoform of protein kinase C serves as a factor that integrates with peripheral MOR pathway and insulin signals for glucose utilization . - pubmed
The developments call new insights into the chemical compounds and/or herbal products that might enhance opioid peptide secretion and/or stimulate MOR in peripheral insulin - sensitive tissues to serve as potential agents or adjuvants for helping the glucose metabolism . - pubmed
Role of rostral ventrolateral medullary ERK / JNK / p38 MAPK signaling in the pressor effects of ethanol and its oxidative product acetaldehyde . - pubmed
METHODS : Effects of EtOH or ACA on blood pressure ( BP ) were evaluated in the absence or presence of selective JNK ( SP600125 ) , ERK ( PD98059 ) , p38 ( SB203580 ) , or ser / thr phosphatases ( okadaic acid [ OKA ] ) inhibitor . - pubmed
SP600125 abrogated the pressor action of EtOH , but not ACA , thus implicating JNK in EtOH action on BP . - pubmed
Despite EtOH enhancement of p38 phosphorylation , pharmacological studies argued against a causal role for this kinase in EtOH - evoked pressor response . - pubmed
Interestingly , pharmacologic phosphatase inhibition ( OKA ) , which increased RVLM p - ERK2 and BP , abrogated the pressor effect of subsequently administered EtOH or ACA . - pubmed
CONCLUSIONS : Enhancement of RVLM ERK2 phosphorylation constitutes a major molecular mechanism for the pressor response elicited by intra - RVLM EtOH or its metabolite , ACA , in conscious SHRs . - pubmed
Pharmacological inhibition of GSNOR is being actively pursued as a therapeutic approach to increase S - nitrosoglutathione levels for the treatment of asthma and cystic fibrosis . - pubmed
Other than being comprised of micrometer - sized aggregates that include nanoscale particulates , Alum lacks specific physicochemical properties to explain activation of the innate immune system , including the mechanism by which aluminum - based adjuvants engage the NLRP3 inflammasome and IL-1beta production . - pubmed
Because we know that long aspect ratio nanomaterials trigger the NLRP3 inflammasome , we synthesized a library of aluminum oxyhydroxide ( AlOOH ) nanorods to determine whether control of the material shape and crystalline properties could be used to quantitatively assess NLRP3 inflammasome activation and linkage of the cellular response to the material 's adjuvant activities in vivo . - pubmed
These results demonstrate that shape , crystallinity , and hydroxyl content play an important role in NLRP3 inflammasome activation and are therefore useful for quantitative boosting of antigen - specific immune responses . - pubmed
Furthermore , the transformation of strains with wcjE alleles from 11Av strains was sufficient to restore partial betaGal6OAc in an 11E background . - pubmed
IDH2 encodes an enzyme in the tricarboxylic acid cycle in the mitochondrion , which plays a key role in aerobic energy metabolism . - pubmed
Single Nucleotide Polymorphisms ( SNPs ) in genes involved in the DNA Base Excision Repair ( BER ) pathway could be associated with cancer risk in carriers of mutations in the high - penetrance susceptibility genes BRCA1 and BRCA2 , given the relation of synthetic lethality that exists between one of the components of the BER pathway , PARP1 ( poly ADP ribose polymerase ) , and both BRCA1 and BRCA2 . - pubmed
Lysyl oxidase promotes bleomycin - induced lung fibrosis through modulating inflammation . - pubmed
LOX expression is significantly upregulated in bleomycin ( BLM)-induced lung fibrosis , and knockdown of LOX expression or inhibition of LOX activity alleviates the lung fibrosis . - pubmed
AIM : To evaluate the efficacy and safety of arsenic trioxide transarterial chemoembolization and intravenous administration in unresectable hepatocellular carcinoma with lung metastasis . - pubmed
GPI inhibited ADR - induced apoptosis accompanied by decreased Fas and increased Survivin in RA FLS . - pubmed
Neurotensin ( NT ) is a neurohormone produced in the central nervous system and in the gut epithelium by the enteroendocrine N cell . - pubmed
Luminal inhibition of the sodium - glucose cotransporter 1 ( SGLT1 ) ( by phloridzin ) eliminated glucose - stimulated release as well as secretion stimulated by luminal methyl - alpha - D - glucopyranoside ( 20 % wt / vol ) , a metabolically inactive SGLT1 substrate , suggesting that glucose stimulates secretion by initial uptake by this transporter . - pubmed
However , secretion was also sensitive to GLUT2 inhibition ( by phloretin ) and blockage of oxidative phosphorylation ( 2 - 4 - dinitrophenol ) . - pubmed
Therefore , glucose stimulates NT secretion by uptake through SGLT1 and GLUT2 , both causing depolarization either as a consequence of sodium - coupled uptake ( SGLT1 ) or by closure of KATP channels ( GLUT2 and SGLT1 ) secondary to the ATP - generating metabolism of glucose . - pubmed
The influence of zinc ( II ) on thioredoxin / glutathione disulfide exchange : QM / MM studies to explore how zinc ( II ) accelerates exchange in higher dielectric environments . - pubmed
QM / MM studies were performed to explore the energetics of exchange reactions of glutathione disulfide ( GSSG ) and the active site of thioredoxin [ Cys32 - Gly33 - Pro34 - Cys35 ] with and without zinc ( II ) , in vacuum and solvated models . - pubmed
Surface morphology and porous structure of the PSi were characterized using scanning electron microscopy ( SEM ) and transmission electron microscopy ( TEM ) . - pubmed
The expression of SRSF3 in DMBA treated primary cultured oral epithelial cells were analyzed by western blot . - pubmed
Carcinogen DMBA treated primary cultured oral epithelial cells showed significantly increased SRSF3 level than in control cells . - pubmed
The Renal Sodium Bicarbonate Cotransporter NBCe2 : Is It a Major Contributor to Sodium and pH Homeostasis ? - pubmed
Subsequently , NBCe2 was found in diverse locations where it plays a role in regulating sodium and bicarbonate transport , influencing intracellular , extracellular , interstitial , and ultimately plasma pH ( Boron et al . - pubmed
NBCe2 is located in the proximal tubule Golgi apparatus under basal conditions and then disperses throughout the cell , but particularly into the apical membrane microvilli , during various maneuvers that increase intracellular sodium . - pubmed
Depot medroxyprogesterone acetate use associated with increased hemoglobin and immune activation ( HBD , HBB , IL36 G ) , and decreased epithelial repair proteins ( TFF3 , F11R ) . - pubmed
Two advantages of the new procedure are : ( a ) no benzyl ester was formed and ( b ) a stereoisomeric mixture of syn - and anti - alcohols at the beginning of the synthetic sequence could be separated and the rest of the synthesis could be carried out with the main syn - stereoisomer instead of the corresponding stereoisomeric mixture as it was the case in the previous process . - pubmed
Fimasartan is a novel angiotensin II receptor blocker . - pubmed
Monitoring of the surface reaction and characterization of the adsorbed heptacene was performed with scanning tunneling microscopy ( STM ) , X - ray photoelectron spectroscopy ( XPS ) , near - edge X - ray absorption fine structure ( NEXAFS ) spectroscopy , and density functional theory ( DFT ) calculations . - pubmed
In the STM images , heptacene shows a dumbbell shape , which may correspond to the substantial out - of - plane deformations of heptacene on Ag ( 111 ) . - pubmed
The anti - aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells . - pubmed
Here , we report that GABA markedly increased circulating levels of Klotho in streptozotocin ( STZ)-induced diabetes . - pubmed
NF - kappaB activation triggers beta - cell apoptosis , and both GABA and Klotho suppress this pathway . - pubmed
We found Klotho KD augmented NF - kappaB p65 expression , and abrogated the ability of GABA to block NF - kappaB activation . - pubmed
Resveratrol can improve the dysfunction by downregulating the mast cell activation and the activity of TGF - beta / Wnt / beta - catenin pathway . - pubmed
The purpose of this study was to develop and test a nonlinear optical device to photoactivate riboflavin to produce spatially controlled collagen crosslinking and mechanical stiffening within the cornea . - pubmed
A 2.3 nm long perylene tetracarboxylic acid diimide ( PDI ) acceptor molecule equipped with isocyanide linker groups was synthesized , adsorbed onto Ag , Au and Pt substrates , and the current - voltage ( I - V ) properties were measured by CP - AFM . - pubmed
Naloxone , beta funaltrexamine , or naltrindole was used with 1 muM morphine to assess the involvement of specific opioid receptor . - pubmed
A p38 mitogen - activated protein kinase ( MAPK ) activator ( anisomycin 0.1 muM ) or inhibitor ( SB 203580 , 20 muM ) was used to determine the involvement of p38 MAPK pathway . - pubmed
Results Morphine decreased lipopolysaccharide and interferon - gamma - induced microglial engulfment except the highest concentration ( 10 muM ) and both naloxone and naltrindole ( a selective delta opioid receptor antagonist ) attenuated morphine effect ( p & lt ; 0.001 ) . - pubmed
SB203580 blocked the increased microglial engulfment induced by lipopolysaccharide and interferon - gamma ( p & lt ; 0.001 ) ; whereas , anisomycin enhanced the morphine - induced decrease of engulfment ( p & lt ; 0.001 ) . - pubmed
This morphine effect seems to be mediated by delta opioid receptor and via p38 MAPK inhibition . - pubmed
When the ethanol to water ratio was 10:1 , the diameter of the spherical polysaccharide nanoparticles was the smallest , and the mean particle size of the TP - NPs , GLP - NPs , and MCP - NPs was 99 + - 15 , 95 + - 7 , and 141 + - 9 nm , respectively . - pubmed
We found that red and blue light could enhance artemisinin accumulation by promoting the expression of the genes that were involved in artemisinin biosynthesis , such as amorpha-4,11 - diene synthase ( ADS ) and cytochrome P450 monooxygenase ( CYP71AV1 ) genes . - pubmed
18F - FDG PET / CT Unveiling of Implant Rupture and Clinically Unsuspected Silicone Granuloma in Treated Breast Cancer . - pubmed
The fatty acid profile ( & gt;5 % ) included C18 : 1omega6c ( 43.1 % ) , C16 : 0 ( 14.6 % ) , C17 : 1omega6c ( 14.0 % ) and C14 : 0 2 - OH ( 11.1 % ) . - pubmed
Bioflavonoids promote stable translocations between MLL - AF9 breakpoint cluster regions independent of normal chromosomal context : Model system to screen environmental risks . - pubmed
Overlap of MLL bcr sequences associated with both infant acute leukemia and therapy - related leukemia following exposure to the topoisomerase II inhibitor etoposide led to the hypothesis that exposure during pregnancy to biochemically similar compounds may promote infant acute leukemia . - pubmed
We show bioflavonoids genistein and quercetin most biochemically similar to etoposide have a strong association with MLL - AF9 bcr translocations , while kaempferol , fisetin , flavone , and myricetin have a weak but consistent association , and other compounds have a minimal association in both embryonic stem ( ES ) and hematopoietic stem cell ( HSC ) populations . - pubmed
The MLL and AF9 bcrs are sensitive to these agents and recombinogenic independent of their native context suggesting bcr sequences themselves are drivers of illegitimate DNA repair reactions and translocations , not generation of functional oncogenic fusions . - pubmed
Hematoxylin and eosin staining , oil red O staining , immunohistochemistry , immunoblotting , and quantitative polymerase chain reaction ( qPCR ) were performed for mechanistic investigations . - pubmed
Here , we found NGLGN3 was significantly upregulated in neuroblastoma cells and tissues , its overexpression significantly promoted neuroblastoma cell proliferation and growth determined by MTT analysis , colony formation assay , cell cycle progression analysis , BrdU incorporation assay and animal model , while its knockdown inhibited cell proliferation and growth . - pubmed
The RNA polymerase I inhibitor cx-5461 induced apoptosis , ROS production , and proliferative inhibition in the HASMCs , which was partly attenuated by p53 knockout . - pubmed
The specificity of serine / threonine kinases is partly determined by interactions with a few residues near the phospho - acceptor residue , forming the so - called kinase - substrate motif . - pubmed
Comparative clinical study of the efficacy of intralesional MMR vaccine vs intralesional vitamin D injection in treatment of warts . - pubmed
OBJECTIVE : To evaluate the efficacy of intralesional injection of MMR vaccine vs vitamin D in treatment of warts . - pubmed
Group A received intralesional MMR vaccine into largest wart , and group B received intralesional vitamin D3 into each lesion with maximum of five warts treated in one session . - pubmed
CONCLUSIONS : Immunotherapy by both intralesional MMR vaccine and vitamin D3 is simple , well - tolerated , effective , and cost - benefit modalities for the treatment of warts . - pubmed
In this work , we explore the electronic excitation energies of 1,4 - CHD and 1,3 - CHD for both valence and Rydberg states by means of complete active space second - order perturbation theory ( CASPT2 ) , extended multi - state CASPT2 ( XMS - CASPT2 ) , and multiconfiguration pair - density functional theory ( MC - PDFT ) ; it is shown by comparison to experiment that MC - PDFT yields the most accurate results . - pubmed
Parthenolide inhibits lipid accumulation via activation of Nrf2 / Keap1 signaling during adipocyte differentiation . - pubmed
Furthermore , PL significantly reduced intracellular reactive oxygen species ( ROS ) production during adipogenesis . - pubmed
A total number of 5,379 proteins were identified from hemocytes of oysters after the treatments with Ach and ENK separately or simultaneously , and 1,475 of them were found to be significantly up - regulated , while 1,115 of them were significantly down - regulated . - pubmed
The protein expression patterns in the groups treated by ACh and ENK separately were quite similar , which were dramatically different from that in the group treated by ACh+ENK . - pubmed
Moreover , GO enrichment and co - expression network analyses implied that the combined immunomodulation of ACh and ENK might be mediated by p53 , EGF - R - ErbB , and Fc gamma R ( FcgammaR ) signaling pathways . - pubmed
The presented protocol generates MNs within 20 days , with a success rate of 80 % , using specifically chosen molecules , such as Sonic Hedgehog or retinoic acid . - pubmed
The pigment was purified and identified by mass spectrometry ( DI - EI - MS ) and Nuclear Magnetic Resonance ( NMR ) spectroscopy as violacein . - pubmed
Pre- and post - anthracycline cardiac function and hemodynamic variables were compared within each group with paired t - tests ; the change was compared between groups using ANCOVA with adjustment for baseline values . - pubmed
Targeting Caveolin-1 and Claudin-5 with AY9944 , Improve Blood - Brain Barrier Permeability ; Computational Simulation and Experimental Study . - pubmed
The current study aimed to determine the protective effect of AY9944 related to Caveolin-1 and Claudin-5 role in lipid raft , which can rescue the blood - brain barrier from enhanced permeability . - pubmed
Molecular docking simulation demonstrated that AY9944 exerts a possible protective role via attenuating the interaction of the Caveolin-1 and cholesterol in lipid raft . - pubmed
Rescuing blood - brain barrier from enhanced permeability via inducing the protective effect of AY9944 related to caveolin-1 and claudin-5 role in lipid raft was the aim of the current study . - pubmed
In vitro studies carried out in a phosphate buffer saline ( PBS ) solution ( at pH 7.4 or 5.5 ) gave the maximum release levels of active substances after 10 - 240 min depending on the polymer system . - pubmed
The increase in the level of DON decreased the hemoglobin concentration ( p < 0.001 ) , whereas the erythrocyte counts were only decreased at 15 mg DON / kg feed . - pubmed
The enzymes show an absolute requirement for Mn2 + as the divalent ion . - pubmed
The enzymes , however , differ somewhat in the pH curves as well as in the Mn2 + and Mg2 + concentration curves . - pubmed
The method was found to be more sensitive to unstabilized fibrin monomer than the serial - dilution protamine sulfate test , or the ethanol gel test , and detected fibrin in a mixture of the plasmin digestion products of fibrin . - pubmed
In the present study the sensitivity of the immune precipitate method for detecting specific plasmin digestion products of fibrin X , Y , D , and E , its sensitivity for stabilized fibrin monomer complexes , and its sensitivity for fibrin retaining B - peptide were measured and compared with the corresponding sensitivities of the serial - dilution protamine sulfate test and the ethanol gel test for detecting these same products . - pubmed
The immune precipitate method was found to be highly sensitive to stabilized fibrin monomer complexes and to fibrin retaining B - peptide ; to be significantly less sensitive to the plasmin digestion fragments X , Y , and E ; and to be insensitive to fragment D. The serial - dilution protamine sulfate test and the ethanol gel test were found to be insensitive to all of the plasmin digestion products of fibrin and less sensitive to the other fibrin complexes . - pubmed
After GC analysis of the vincamine - serum concentration the following pharmacokinetic profile could be established : The substance appears in the streaming blood with an invasion half - life of 53 min . - pubmed
These studies suggest that the carbohydrate intolerance of cystic fibrosis is consequent upon an impaired insulin response to glucose , but that this insulin deficiency is partly compensated for by increased peripheral tissue sensitivity to insulin . - pubmed
Galactose and galactose-1 - amine were competitive inhibitors of beta - galactosidase . - pubmed
The stability of neuraminidase has been found increase in the presence of magnesium ions . - pubmed
These cleavage fragments from type III and IV collagens were separated from the intact collagen chains by SDS polyacrylamide gradient gel electrophoresis run under reducing conditions , and they were detected by their characteristic Coomassie blue staining pattern . - pubmed
The specific binding of 125 - I - alpha - actinin to vinculin and metavinculin immobilized on a polysterene surface was also demonstrated . - pubmed
Pertussis toxin ( PT ) catalyzes the ADP - ribosylation of several guanine nucleotide - binding ( G ) proteins that are involved in the transduction of cell surface receptor - mediated signals . - pubmed
The toxin effect was blocked by heating ( 95 degrees C for 30 minutes ) , by antitoxin antibody and was not associated with increased cAMP levels in target cells . - pubmed
Catalytically active immobilized dimer and monomeric forms of the enzyme were prepared after urea - induced dissociation of the tetramer . - pubmed
A study of the coenzyme - binding properties of matrix - bound tetrameric , dimeric and monomeric species has shown that : ( 1 ) an immobilized tetramer binds NAD+ with negative cooperativity , the dissociation constants being 0.085 microM for the first two coenzyme molecules and 1.3 microM for the third and the fourth one ; ( 2 ) coenzyme binding to the dimeric enzyme form also displays negative cooperativity with Kd values of 0.032 microM and 1.1 microM for the first and second sites , respectively ; ( 3 ) the binding of NAD + to a monomer can occur with a dissociation constant of 1.6 microM which is close to the Kd value for low - affinity coenzyme binding sites of the tetrameric or dimeric enzyme forms . - pubmed
Thus , the monomers of rabbit muscle D - glyceraldehyde-3 - phosphate dehydrogenase displayed a hyperbolic kinetic saturation curve for NAD + , whereas the tetramers exhibited an intermediary plateau region corresponding to half - saturating concentrations of NAD+ . - pubmed
The rate constant of inactivation due to modification of essential arginine residues in the holoenzyme decreased in the presence of glyceraldehyde 3 - phosphate , probably as a result of conformational changes accompanying catalysis . - pubmed
Angiotensin II causes sustained elevations in cytosolic calcium in glomerulosa cells . - pubmed
The threshold concentration for angiotensin II - stimulated increases in cytosolic calcium was 10(-14)-10(-13 ) M , and maximal elevation of cytosolic calcium was produced by 10(-9 ) M angiotensin II . - pubmed
The calcium signals invoked by intermediate concentrations ( 10(-12)-10(-10 ) M ) of angiotensin II exhibited dose - dependent increases in magnitude and a gradual transition in nature between those invoked by threshold and maximal concentrations of the peptide . - pubmed
Significant resistance against oral T. gondii infection was induced by intraperitoneal pretreatment with TDM but not with MDP . - pubmed
The apparent Km for ATP in soluble human adrenal glycerol kinase was 22.0 microM. - pubmed
All D- and L - isomers of amino acids tested were found to markedly stimulate pepsin secretion , but to only slightly stimulate acid secretion . - pubmed
Pepsin stimulatory response to each D and L - isomer of amino acid was similar . - pubmed
In contrast to the conformation in aqueous solution , the C6 hydroxymethylene group is freely rotating around the C6 - - C5 bond . - pubmed
The large difference in coupling constants of the methylene protons of C1 ' to the C2 ' methine proton of the sphingosine indicates a restricted rotation around the C1'--C2 ' bond . - pubmed
The Ca ( 2 +)-binding constant of the C - terminal site in the Asn-56 --> ; Ala mutant of calbindin D9k has been measured at seven different protein concentrations ranging from 27 microM to 7.35 mM by using 1H NMR . - pubmed
For calmodulin , we have measured the average Mg ( 2 +)-binding constant of sites I and II at 0.325 , 1.08 , and 3.25 mM protein and find a 13 - fold difference between the two extremes . - pubmed
Specific species and tissue differences for the gene expression of calcium - binding protein regucalcin . - pubmed
Addition to the dialysis perfusion solution of either D1 - R and D2 - R agonists , such as SKF-38393 ( 50 and 100 microM ) and Quinpirole ( 5 and 10 microM ) , resulted in dose - dependent increases in extracellular concentrations of GLU and ASP , respectively . - pubmed
The SKF-38393 and Quinpirole - induced effects were reduced by SCH-23390 ( 0.5 microM ) , a D1 - R antagonist , and by Spiperone ( 1.0 microM ) , a D2 - R antagonist , respectively . - pubmed
However , SCH-23390 and Spiperone did increase GLU and ASP extracellular concentrations . - pubmed
No differences were observed among women with primary ( no full term pregnancy ) or secondary abortions ( some full term pregnancies ) with regard to common HLA antigens , with both groups being homogeneous . - pubmed
Furosemide ( 30 mg / kg ) was given orally with or without pretreatment with derapril ( 30 mg / kg ) , an angiotensin converting enzyme inhibitor , TCV-116 ( 1 mg / kg ) , an AT1 receptor antagonist , or losartan ( 10 mg / kg ) , another AT1 receptor antagonist . - pubmed
As TCV-116 and losartan are selective AT1 receptor antagonists , the effect of furosemide on renal PGE2 production , as reflected by the urinary PGE2 , might be mediated by an activation of AT1 receptors . - pubmed
Gastrin did not induce cell proliferation or increase [ 3H ] thymidine incorporation and no high - affinity gastrin binding sites were observed . - pubmed
An assay to detect glycoproteins that contain mannose 6 - phosphate . - pubmed
We have developed a broadly applicable solid phase assay for quantitative and qualitative analysis of phosphorylated lysosomal enzymes and other glycoproteins that contain mannose 6 - phosphate ( Man6 - P ) residues . - pubmed
Samples are immobilized on membranes and proteins that contain Man6 - P are detected using an iodinated , soluble form of the cation - independent Man6 - P receptor . - pubmed
Second , it can be used in a western blot format to identify Man6 - P glycoproteins that have been fractionated by polyacrylamide gel electrophoresis . - pubmed
No correlation could be found between sE - selectin concentrations and Gd enhancement on MRI , but a close correlation existed between mean concentrations of sE - selectin and TNF alpha ( r = 0.71 , P & lt ; 0.001 ) . - pubmed
Raised CRP concentrations did correlate with infectious episodes , clinical relapse , and Gd enhancement , and were significantly raised when no MRI activity was found . - pubmed
The expression of aromatase by breast cancer cells and the role of locally produced estrogen in the stimulation of tumor growth has been controversial . - pubmed
The functional significance of locally produced estrogens on growth of the tumor was addressed by measuring aromatase activity and a marker of proliferation ( PCNA score ) . - pubmed
In the ten tumors that showed immunoreaction to aromatase , the average aromatase activity measured in cryosections was 286.5 + /- 18.6 ( SE ) fmol estrogen / mg protein.h , whereas in nine tumors with weak aromatase immunoreaction , the enzyme activity was 154.7 + /- 19.3 ( SE ) fmol estrogen / mg protein - h ( P & lt ; 0.05 ) . - pubmed
In two of these tumors , aromatase activity was high and [ 3H ]- thymidine incorporation into DNA was also stimulated by testosterone . - pubmed
In the other two tumors that had low aromatase activity , no such stimulation occurred with testosterone . - pubmed
The results indicate that aromatase is expressed mainly in tumor epithelial cells and that sufficient amounts of estrogen are synthesized by the tumor to produce a proliferative response . - pubmed
We demonstrate that binding studies using [ 125I ] HPP - HOE140 offer high sensitivity and specificity for characterization , quantitation and localization of the bradykinin B2 receptors in tissues and offers new information on uterine bradykinin B2 receptors . - pubmed
13 RA , 23 osteoarthritis ( OA ) , 21 joint trauma ( JT ) , and 9 synovial sarcoma ( SS ) specimens were immunostained for Ki-67 and PCNA , silver - stained for nucleolar organizer regions ( AgNORs ) , and double stained with either T - cell- ( CD3 ) , macrophage- ( CD68 ) , or polymorphonuclear neutrophilic leucocytes ( PMN)-markers ( CD15 ) . - pubmed
The mean number of abnormal psychometric test results and the prevalence of SHE were used for a quantitative evaluation of the efficacy of the lactulose treatment . - pubmed
Treatment patterns and distribution of low - density lipoprotein cholesterol levels in treatment - eligible United States adults . - pubmed
To estimate the fraction of United States ( U.S. ) adults who are eligible for treatment to reduce elevated low - density lipoprotein ( LDL ) cholesterol levels based on Adult Treatment Panel II ( ATP II ) guidelines and the percent reduction in LDL cholesterol required by those who qualify for treatment , we analyzed data on 7,423 respondents to Phase 2 of the third National Health and Nutrition Examination Survey ( NHANES III ) administered between 1991 and 1994 . - pubmed
Eighty - two percent of adults with coronary heart disease are not at their target LDL cholesterol level of 100 mg / dl . - pubmed
Overall , 40 % of people who qualify for drug therapy require an LDL cholesterol reduction of & gt ; 30 % to meet their ATP II treatment goal . - pubmed
Approximately 75 % of those with coronary heart disease who qualify for drug therapy require an LDL cholesterol reduction of & gt;30 % . - pubmed
Although elevated LDL cholesterol levels can be treated , prevalence rates in the U.S. adult population remain high . - pubmed
The findings in this study provide at least a partial explanation for why many patients receiving therapy do not reach their treatment goals : they require a larger reduction in LDL cholesterol than many therapies can provide . - pubmed
The first reaction binds UDP - glucose ( UDP - Glu ) , displaces glucose-1 - phosphate ( glu-1 - P ) , and forms the UMP - GALT intermediate . - pubmed
In the second reaction , galactose-1 - phosphate ( gal-1 - P ) is bound , UDP - galactose ( UDP - Gal ) is released , and the free enzyme is recycled . - pubmed
Gln188 - GALT displaced 80 + /- 7 . - pubmed
By contrast , both Arg188- and Asn188 - GALT released more glu-1 - P ( 170 + /- 8.0 and 129 + /- 28.4 nmol / mg GALT / min , respectively ) . - pubmed
Trials were reviewed that compared calcitonin with placebo , no therapy , or calcium with or without vitamin D , and that mentioned fracture as an outcome . - pubmed
The fungus - inducible PepThi gene is highly inducible only in the unripe fruit by salicylic acid . - pubmed
Expression of the jl - l gene is enhanced by jasmonic acid in the unripe fruit but suppressed in the ripe fruit . - pubmed
The amount of phosphorous in the protein increased with the excess of phosphorous oxychloride and the pH of reaction . - pubmed
The bulk of phosphorus was only loosely bound to the protein and was removed by washing with cold perchloric acid . - pubmed
The more stably bound phosphorus groups after reaction at pH 8 were found to be nearly equally attached to amino and hydroxyl groups , whereas phosphorylation at pH 10 - 11 led to predominant O - phosphorylation as detected by studying the acid- and alkali - lability of the protein - phosphorous bonds . - pubmed
Similar losses in hepatic enzyme were observed for benzyloxy - resorufin and pentoxy - resorufin dealkylase ( CYP2B ) and chlorzoxazone hydroxylation ( CYP2E ) . - pubmed
We conclude that N - OH - PABA decreases NAT1 activity by a direct interaction with the enzyme and appears to be a result of covalent modification at the cofactor - binding site . - pubmed
This is in contrast to our findings for PABA , which appears to reduce NAT1 activity by down - regulating the enzyme , leading to a decrease in NAT1 protein content . - pubmed
Urinary excretion of proANP peptides significantly increased , particularly when the serum creatinine level was> ; 5.0 mg / dL [ proANP(1 - 30 ) 26 - fold , proANP(31 - 67 ) 8.4 - fold ] . - pubmed
It is proposed that the diseased kidney increases early urinary excretion of proANP fragments to participate in the regulation of renal function as well as sodium and water excretion . - pubmed
tuberculosis antigens was observed between the DRB1 * 1501 , * 1502 and * 1503 positive or negative controls , ATB and ITB patients . - pubmed
Amounts of ADAM15 protein ( 102+/-12 versus 40+/-6 U ; P<0.01 ) and mRNA ( 24.0+/-5.6 versus 10.5+/-2.5 U ; P<0.05 ) increased significantly during AF compared with sinus rhythm . - pubmed
Causes of interscan variability of coronary artery calcium measurements at electron - beam CT . - pubmed
RATIONALE AND OBJECTIVES : The authors performed this study to investigate the causes of interscan variability of coronary artery calcium measurements at electron - beam computed tomography ( CT ) . - pubmed
Indeed , we show that fluid intake had a significant impact : albumin concentration decreased , whereas the albumin / creatinine ratio ( A / C ) and albumin excretion rate increased ( P<.001 ) significantly . - pubmed
Following 12 weeks of diabetes , there was a small increase in the level of glutamine synthetase labelling in somata and endfeet compared with controls ( ANOVA , P & lt ; 0.05 ) . - pubmed
ACBP and cholesterol differentially alter fatty acyl CoA utilization by microsomal ACAT . - pubmed
Microsomal acyl CoA : cholesterol acyltransferase ( ACAT ) is stimulated in vitro and/or in intact cells by proteins that bind and transfer both substrates , cholesterol , and fatty acyl CoA. - pubmed
To resolve the role of fatty acyl CoA binding independent of cholesterol binding / transfer , a protein that exclusively binds fatty acyl CoA ( acyl CoA binding protein , ACBP ) was compared . - pubmed
In the presence of exogenous cholesterol , ACAT was stimulated in the order : ACBP & gt ; sterol carrier protein-2 ( SCP-2 ) & gt ; liver fatty acid binding protein ( L - FABP ) . - pubmed
In contrast , in the absence of exogenous cholesterol , these proteins inhibited microsomal ACAT , but in the same order : ACBP & gt ; SCP-2 & gt ; L - FABP . - pubmed
The extracellular protein BSA stimulated microsomal ACAT regardless of the presence or absence of exogenous cholesterol . - pubmed
Thus , ACBP was the most potent intracellular fatty acyl CoA binding protein in differentially modulating the activity of microsomal ACAT to form cholesteryl esters independent of cholesterol binding / transfer ability . - pubmed
The enzyme showed a high dioxygenase activity only for 1,2,4 - trihydroxybenzene , with K(m ) and V(max ) values of 9.6 micro M and 6.8 micro mol min(-1 ) mg of protein(-1 ) , respectively . - pubmed
OBJECTIVE : To evaluate the short - term bone fusion after implantation of composite biosynthetic bone made of coralline hydroxyapatite composite ( CHC ) as a substitute for autologous bone grafting . - pubmed
[ The effect of diphenylmethane derivatives and of a basic alkyl derivative of o -( 4 - hydroxybenzoyl )- benzoic acid methyl ester on the uterine muscle and on the action of hypophysin ] . - pubmed
The tritium rate effect in tyrosinase - catalyzed hydroxylation . - pubmed
Involvement of hepatic glucocorticoid receptor - mediated functions in steroid - induced cataract formation . - pubmed
Determination of whether the steroid - induced cataract formation is caused through glucocorticoid ( GC ) receptor - mediated process was conducted by using GC antagonist ( RU486 ) and anti - GC receptor antibody , and by sucrose density gradient ultracentrifugation analysis . - pubmed
( 1 ) When 15 day - old chick embryos were treated with dexamethasone ( DEX , 0.025 micromol per egg ) , their lenses started to form an opaque ring around the peri - nuclear region ( stage II - III ) after 12 hr and developed into nuclear - like cataract ( stage IV - V ) after 44 hr . - pubmed
( 3 ) Sucrose gradient ultracentrifugation analysis indicated that the receptor ( 9S ) in the liver could be transformed to the 4S form after 0.4 M NaCl treatment . - pubmed
The Gr5a gene on the X chromosome , which encodes a receptor for the sugar trehalose , has transposed from one such tandem cluster of six genes at cytological location 64 , as has Gr61a , and all eight of these receptors might bind sugars . - pubmed
The differential effects of angiotensin II receptors inhibitors losartan and PD123319 on the pancreatic injury were assessed by virtue of using the pancreatic water content , biochemical and histological analyses . - pubmed
Blockade of the AT ( 1 ) receptor by losartan at a dose of 200microg / kg could markedly ameliorate the pancreatic injury induced by cerulein , as evidenced by biochemical and histopathological studies . - pubmed
However , blockade of the AT ( 2 ) receptor by PD123319 appeared not to provide any beneficial role in cerulein - induced pancreatic injury . - pubmed
The protective action of losartan was linked to an inhibition of NAD ( P ) H oxidase activity , thus consequential oxidative modification of pancreatic proteins in the pancreas . - pubmed
Thus the protective role of AT ( 1 ) receptor antagonist , losartan , could be mediated by the inhibition of NAD ( P ) H oxidase - dependent generation of reactive oxygen species ( ROS ) . - pubmed
At MRS , cancer patterns were diagnosed when the ratio of choline plus creatine to citrate was greater than 0.86 . - pubmed
[ Studies on alkaline phosphatases in osteoarticular tuberculosis and effect of streptomycin therapy ] . - pubmed
At the dose of 50 mg / kg , riparin III increased the number of entries in the open arms of the EPM test as compared with control . - pubmed
The subunit that contains the sulfate ( or phosphate ) ion and two magnesium ions is in the closed conformation observed in enolase complexes with the substrate or its analogues ; the other subunit is in the open conformation observed in enolase subunits without bound substrate or analogues . - pubmed
Although fibric acid derivatives increase serum HDL - C levels and decrease triglyceride levels , their effects on renal function are largely unknown . - pubmed
During positive ion LC / ESI - MS , LG formed an abundant sodiated species ( [ M+Na]+ ) while the protonated molecule was barely observable . - pubmed
Neither antibody blocking procedures nor an avidin / biotin block were entirely satisfactory for eliminating this background staining . - pubmed
Prophenin-2 ( PF-2 ) is a cathelicidin , 97 - amino - acid antimicrobial protein stored in neutrophil secondary granules . - pubmed
CONCLUSIONS : The treatment intensity achieved with PEG ASNase in the present study appears to be acceptable based on the surrogate of serum ASNase activity . - pubmed
Treatment of leaves with butanedione monoxime , an inhibitor of myosin ATPase , resulted in similar behaviour of mitochondria to that under ROS stress . - pubmed
It seems that neutrophil migration impairment is mediated by the circulating inflammatory cytokines , such as TNF - alpha and IL-8 , which induce the nitric oxide ( NO ) production systemically . - pubmed
In conclusion , we demonstrated that , in sepsis , failure of neutrophil migration is critical for the outcome and that NO is involved in the process . - pubmed
The method is based on derivatization of JA with 5 - bromomethylfluorescein ( 5 - BMF ) and separation and quantification of the resulting 5 - BMF - JA derivative by capillary electrophoresis coupled to laser - induced fluorescence detection ( CE - LIF ) . - pubmed
Expression of the collagen type II gene was selectively upregulated for PGA , PGCL , and PLGA and slightly increased for P(LA - CL)25 polymers but was downregulated for fullerene C-60 DMA . - pubmed
Aggrecan gene expression was strongest with PGA and was consistently expressed with other matrices , especially with PGCL and PLGA . - pubmed
We found the relationship between the 3 ' end ACCN sequences of tRNA molecules and the activated amino acids and its biological significance , the rRNAs ' consensus sequences 5'NCC ... TGG3 ' or 5'TGG ... NCC3 ' which may perform as functional segments of rRNAs to help triggering the reaction of peptide formation , and common nature of releasing factors that the first amino acid residue of releasing factors ERF , RF1 and RF2 are all Methionine , except a few Alanine , which may be necessary for releasing the translated polypeptide and stopping the translating process . - pubmed
There was an increase in the levels of fructose 1,6 - P ( 2 ) , sedoheptulose 1,7 - P ( 2 ) , triose - P , ribose 5 - P , and ribulose 5 - P pools , indicating inactivation of stromal enzymes , most notably fructose 1,6 - bisphosphatase , sedoheptulose 1,7 - bisphosphatase , and ribulose 5 - P kinase . - pubmed
The mechanisms of inflammation include cellular peroxide production , acidic solutions , glucose and its degradation products , and the integrated response of the cellular - cytokine cascade . - pubmed
Cardiovascular and antiinflammatory therapeutics may interfere with TGF and thereby influence the effect of thiazides once co - administration is clinically indicated . - pubmed
Similar results were obtained with the AT II type 1 receptor antagonist candesartan ( 8 mg ): GFR remained stable , Na (+ ) excretion rose by 35 % and K (+ ) excretion was not changed . - pubmed
The effect of the Ca ( 2 + ) channel blocker amlodipine ( 5 mg ) on GFR and HCT - induced Na (+ ) excretion equalled that obtained with the AT ( 1 ) blocker , yet with this treatment K (+ ) excretion rose in proportion to Na (+ ) excretion . - pubmed
The beta - blockers propranolol ( 80 mg ) or bisoprolol ( 5 mg ) reduced GFR but maintained TGF . - pubmed
The inhibition of cyclooxygenase by diclofenac ( 50 mg ) or rofecoxib ( 25 mg ) significantly reduced the diuretic / natriuretic effect of HCT , but K (+ ) excretion was unchanged , and TGF was still demonstrable . - pubmed
CONCLUSION : In conclusion , AT ( 1 ) receptors , as well as the Ca ( 2 + ) channels in the smooth muscle cells of the afferent arteriole , are considered prerequisites for TGF function ; their blockade increases the diuretic / natriuretic efficacy of thiazide diuretics . - pubmed
Resveratrol binds to the sulfonylurea receptor ( SUR ) and induces apoptosis in a SUR subtype - specific manner . - pubmed
Sulfonylurea receptors ( SURs ) constitute the regulatory subunits of ATP - sensitive K + channels ( K(ATP ) channels ) . - pubmed
Optimization of halopemide for phospholipase D2 inhibition . - pubmed
Halopemide , which was identified by HTS to inhibit phospholipase D2 ( PLD2 ) , provided the basis for an exploratory effort to identify potent inhibitors of PLD2 for use as inflammatory mediators . - pubmed
Parallel synthesis and purification were utilized to rapidly identify orally available amide analogs derived from indole 2 - carboxylic acids with superior potency versus PLD2 . - pubmed
Site - directed mutagenesis analysis demonstrated that the GxGK(232)T to GxGAT mutation in the conserved motif I abolished the NTPase activity of ntNS3 , whereas substitution of TATPA(354 ) for TATPV in the motif III had no effect on the enzyme activity . - pubmed
Strychnine - sensitive glycine receptors mediate the analgesic but not hypnotic effects of emulsified volatile anesthetics . - pubmed
These results suggest that strychnine - sensitive glycine receptors may contribute to the analgesic but not to the hypnotic effects induced by ether , enflurane , isoflurane and sevoflurane . - pubmed
In recent years , the molecular structures of Ngb with carbon monoxide bound to the heme iron and without an exogenous ligand have been solved , and interesting structural changes have been noticed upon ligand binding . - pubmed
A decrease in intracellular magnesium , caused by magnesium deficiency , releases the magnesium - mediated inhibition of ROMK channels and increases potassium secretion . - pubmed
Heparin attenuates metastasis mainly due to inhibition of P - and L - selectin , but non - anticoagulant heparins can have additional effects . - pubmed
The virtual elimination of metastasis by a single treatment with a modified heparin without anticoagulant activity strongly suggests that heparin primarily reduces metastatic disease by inhibiting P - and L - selectin interactions . - pubmed
An important regulatory mechanism of this complex is through acetylation and SIRT1 - mediated lysine de - acetylation under low nutrient conditions . - pubmed
RESULTS : Increased uptake levels of 18F - FDG were observed in the esophagi after EDA procedure . - pubmed
Moreover , the adenosine receptor antagonist , caffeine , potently attenuates the EEG markers of nonREM sleep homeostasis during sleep , as well as during wakefulness . - pubmed
While these convergent findings strongly support an important contribution of adenosine and adenosine receptors to nonREM sleep homeostasis , further research is needed to elucidate the underlying mechanisms that mediate the actions of adenosine on sleep and the sleep EEG . - pubmed
The biologic agent bevacizumab ( Avastin ) , an inhibitor against vascular endothelial growth factor , and cetuximab ( Erbitux ) and panitumumab ( Vectibix ) , monoclonal antibodies to epidermal growth factor receptor , have demonstrated their efficacy in clinical trials . - pubmed
In the present study , we show that ISG15 is a determinant for CPT sensitivity / resistance possibly through its effect on proteasome - mediated repair of topoisomerase I ( TOP1)-DNA covalent complexes . - pubmed
Second , the level of ISG15 was found to be significantly reduced in several tumor cells selected for resistance to CPT , suggesting that altered ISG15 regulation could be a significant determinant for acquired CPT resistance . - pubmed
Taken together , these results suggest that ISG15 , which interferes with proteasome - mediated repair of TOP1 - DNA covalent complexes , is a potential tumor biomarker for CPT sensitivity . - pubmed
Posttranslational carboxyl methylation of small GTPases , such as RhoA and Ras , regulates subcellular localization and GTPase activity , resulting in altered cellular function . - pubmed
In this study , we investigated the role of RhoA carboxyl methylation in modulating endothelial monolayer permeability . - pubmed
These results suggest that ICMT modulates endothelial monolayer permeability by altering RhoA carboxyl methylation and activation . - pubmed
The APOE gene was found to be of bimodal structure , with a hypomethylated CpG - poor promoter and a fully methylated 3'-CpG - island , that contains the sequences for the epsilon4 - haplotype , which is the only undisputed genetic risk factor for LOAD . - pubmed
The concentrations of cytokines [ interleukin ( IL )- 4 , IL-10 , IL-8 , IL-6 , tumor necrosis factor - alpha ( TNFalpha ) , IL-1beta , interferon - gamma ) were assessed by ELISA , and nitric oxide ( NO ) production was evaluated by Griess assay . - pubmed
Arg(high)/Gln(high ) decreased the production of TNFalpha , IL-1beta , IL-8 , and IL-6 ( each P & lt ; 0.01 ) . - pubmed
Arg(low)/Gln(high ) decreased IL-6 and IL-8 production ( both P & lt ; 0.01 ) , whereas Arg(high)/Gln(low ) did not affect cytokine and NO production . - pubmed
Arg(low)/Gln(high ) and Arg(high)/Gln(high ) decreased NF - kappaB p65 subunit expression , whereas p38 MAPK was decreased only by Arg(high)/Gln(high ) . - pubmed
Combined pharmacological doses of Arg and Gln decreased TNFalpha and the main proinflammatory cytokines release in active colonic CD biopsies via NF - kappaB and p38 MAPK pathways . - pubmed
We then administered physiologic doses of thyroxine to inhibit thyroid - stimulating hormone secretion . - pubmed
Mutagenesis of key arginine residues in the DNA contact sequence results in the loss of specific interaction of the PEST sequence with the p65 subdomain . - pubmed
We conclude that the local structure of the IkappaBalpha / NF - kappaB complex in the region of the PEST sequence is consistent with a direct interaction of this acidic sequence with the basic DNA contact sequence in p65 , thus reducing the affinity of NF - kappaB for DNA by a competitive mechanism that is still to be elucidated fully . - pubmed
Selective estrogen receptor modulators ( SERMs ) , such as tamoxifen ( TAM ) , have been used extensively for the treatment and prevention of breast cancer and other pathologies associated with aberrant estrogen receptor ( ER ) signaling . - pubmed
However , the observation that SERMs can also regulate aspects of calcium signaling and apoptosis in an ER - independent manner in some systems suggests that some of the activity of drugs within this class may also arise as a consequence of their ability to interact with targets other than ER . - pubmed
In this study , we demonstrate that 4 - hydroxy - TAM ( 4OHT ) , an active metabolite of TAM , directly binds to and modulates the transcriptional activity of the aryl hydrocarbon receptor ( AHR ) . - pubmed
Of specific interest was the observation , that in the absence of ER , 4OHT can induce the expression of AHR target genes involved in estradiol metabolism , cellular proliferation , and metastasis in cellular models of breast cancer . - pubmed
The potential role for AHR in SERM pharmacology was further underscored by the ability of 4OHT to suppress osteoclast differentiation in vitro in part through AHR . - pubmed
Cumulatively , these findings provide evidence that it is necessary to reevaluate the relative roles of ER and AHR in manifesting the pharmacological actions and therapeutic efficacy of TAM and other SERMs . - pubmed
Further studies showed that RES also inhibited H ( 2 ) O ( 2 ) induced p38 and JNK phosphorylation . - pubmed
As oxygen concentrations in the UGT are low under physiological conditions ( O(2 ) & lt ; 5 % ) and further decrease during an inflammatory process , we wondered whether antibacterial properties of IFN - gamma are maintained under hypoxic conditions . - pubmed
Impaired effectiveness of IFN - gamma on chlamydial growth under hypoxia was accompanied by reduced phosphorylation of Stat-1 on Tyr701 and diminished IDO activity . - pubmed
trachomatis depend on the environmental oxygen supply , which could explain inadequate bacterial clearance and subsequent chronic infections eventually occurring in the UGT of women . - pubmed
TBADH was active at a water concentration & gt;=10 % v / v. In the reduction reaction of sulcatone to sulcatol carried out in biphasic systems , the yield obtained with SBA-15 - adsorbed TBADH was up to 5.5 - fold higher than that with the free enzyme , which suggests a higher stability of the immobilized enzyme toward the organic solvent . - pubmed
AIMS : To ( i ) determine whether methylarginine - specific antibodies can be employed for standard immunohistochemical analysis of paraffin - embedded tissues , ( ii ) analyse methylarginine expression in normal and neoplastic tissues and ( iii ) correlate methylarginine expression with that of protein arginine methyltransferase ( PRMT1 ) , the predominant cellular arginine methyltransferase . - pubmed
METHODS AND RESULTS : Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies : anti - methylarginine - specific antibody ( anti - mRG ) raised against a methylarginine peptide , Control antibody ( anti - RG ) , a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti - PRMT1 . - pubmed
A correlation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed . - pubmed
CONCLUSION : Methylarginine - specific antibodies are capable of recognizing methylarginine proteins ( MeRP ) in paraffin - embedded tissues . - pubmed
The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins ( MeRP ) in biological processes including carcinogenesis . - pubmed
)-induced elevation of plasma catecholamines was reduced by dihydro - beta - erythroidine ( a selective antagonist of alpha4beta2 nicotinic acetylcholine receptors ) ( 100 and 300nmol / animal , i.c.v . ) - pubmed
, while methyllycaconitine ( a selective antagonist of alpha7 nicotinic acetylcholine receptors ) ( 100 and 300nmol / animal , i.c.v . ) - pubmed
RJR-2403 ( a selective agonist of alpha4beta2 nicotinic acetylcholine receptors ) ( 2.5 and 5mumol / animal , i.c.v . ) - pubmed
elevated plasma noradrenaline and adrenaline ( adrenaline>noradrenaline ) , while PNU-282987 ( a selective agonist of alpha7 nicotinic acetylcholine receptors ) ( 2.5 and 5mumol / animal , i.c.v . ) - pubmed
Increased levels of ROS , MDA and CD4(+ ) lymphocyte apoptosis were positively associated with disease activity while decreased levels of GSH and percentage expression of CD4(+ ) lymphocyte were negatively associated with disease activity . - pubmed
The decrease in intracellular levels of GSH was negatively associated with T lymphocyte , CD4(+ ) lymphocyte , CD8(+ ) lymphocyte apoptosis and intracellular caspase-3 expression . - pubmed
TEM , SEM and EDX analyses indicated that Ag nanoparticles were successfully deposited onto the surfaces of hollow SiO ( 2 ) beads . - pubmed
Noticeably , ginsenoside Rg(6 ) ( 12 ) and ginsenoside F(4 ) ( 13 ) exhibited particularly inhibitory effect on LPS - induced IL-12 production with the inhibition values of 80 and 82 % ; and ginsenoside ST(1 ) ( 4 ) , ginsenoside SL(2 ) ( 8) , ginsenoside SL(3 ) ( 9 ) , ginsenoside Rh(3 ) ( 14 ) , ginsenoside Rk(2 ) ( 15 ) , and ginsenoside Rs(4 ) ( 18 ) showed moderate effects with inhibition rates of 63 , 65 , 67 , 68 , 71 , 73 , and 67 % , respectively . - pubmed
The results show that the amino - acid distribution probability representing the primary structure of cellulase can predict both optimal pH and temperature , whereas various properties of amino acids related to the primary structure can not do so . - pubmed
RESULTS : Diazoxide inhibited in vitro nitric oxide ( NO ) , tumor necrosis factor alpha ( TNF - alpha ) and interleukin-6 ( IL-6 ) production and inducible nitric oxide synthase ( iNOS ) expression by activated microglia without affecting cyclooxygenase-2 ( COX-2 ) expression and phagocytosis . - pubmed
Diazoxide did not affect the number of infiltrating lymphocytes positive for CD3 and CD20 in the spinal cord . - pubmed
In a multicenter randomised 2 x 16 - week cross - over study ( n = 86 ) , with a 6 - week wash out period preceding and between treatment periods , intravenous alfacalcidol and paricalcitol were given by forced titration ( 50 % dose increase ) every second week , until parathyroid hormone were sufficiently suppressed or ionised calcium and/or phosphate levels were elevated . - pubmed
FGF23 increases renal phosphate excretion and decreases levels of 1,25 - dihydroxyvitamin D. - pubmed
We explored the influence of alfacalcidol and paricalcitol on FGF23 in stored blood samples from the beginning and the end of each treatment period . - pubmed
FGF23 increased significantly and equally during treatment with alfacalcidol and paricalcitol . - pubmed
Furthermore , we found baseline FGF23 to predict PTH levels after 16 weeks of vitamin D analog treatment . - pubmed
gamma - Aminobutyric acid type A ( GABA ( A ) ) receptors are principal targets for most anesthetics . - pubmed
The second aim of the study was to determine whether memory deficits after isoflurane can be reversed by inhibiting alpha5GABA ( A ) receptors . - pubmed
We also sought to determine whether the expression of alpha5GABA ( A ) receptors is necessary for the development of memory dysfunction after isoflurane . - pubmed
Gastric bleeding was not induced by AAD-2004 even at a dose 400 - fold higher than that required to obtain maximal therapeutic efficacy in superoxide dismutase 1(G93A ) . - pubmed
Targeting both mPGES-1 - mediated PGE ( 2 ) and free radicals may be a promising approach to reduce neurodegeneration in ALS and possibly other neurodegenerative diseases . - pubmed
The increased cytotoxicity was associated with a concurrent increase in the number of apoptotic cells and production of reactive nitrogen species ( RNS ) . - pubmed
This can delay subsequent use of radioactive iodine ( RAI ) therapy because the iodine can compete for uptake . - pubmed
Since its discovery in 1995 identifying the NAADP receptor protein / Ca2 + channel has been a major persuit of the Ca2 + signalling community . - pubmed
In their paper ' The N - terminal region of two - pore channel 1 regulates trafficking and activation by NAADP ' published in this issue of the Biochemical Journal Patel and colleagues describe that the N - terminus of one of the NAADP receptor protein / Ca2 + channel candidates , TPC1 ( two - pore channel 1 ) , is crucial for protein targeting and for sensitivity to NAADP . - pubmed
We characterized the binding of GAG heparin octasaccharide to dCXCL1 using solution NMR spectroscopy . - pubmed
Purine derivatives as potent Bruton ' s tyrosine kinase ( BTK ) inhibitors for autoimmune diseases . - pubmed
Investigation of various heterocyclic core isosteres of imidazopyrazines 1 & amp ; 2 yielded purine derivatives 3 & amp ; 8 as potent and selective BTK inhibitors . - pubmed
Subsequent SAR studies of the purine series led to the discovery of 20 as a leading compound . - pubmed
Biochemical investigations with recombinant proteins demonstrated that L - tryptophan and derivatives , including D - tryptophan , 4 - , 5- , 6- and 7 - methyl - dl - tryptophan , were well accepted by both enzymes in the presence of DMAPP . - pubmed
Structural elucidation of the isolated products revealed regiospecific prenylation at C-6 of the indole ring and proved unequivocally the identification of two very similar 6 - dimethylallyltryptophan synthases ( 6 - DMATS ) . - pubmed
Utilizing GPP as a prenyl donor has not been reported for tryptophan prenyltransferases previously . - pubmed
Study variables were tumor weight ( TW ) ; Hb , as index of tumor - mediated angiogenesis ; vascular endothelial growth factor ( VEGF ) protein abundance ; protein carbonyl content ; alpha - tubulin abundance ; and GluAp , AlaAp , and AspAp activities . - pubmed
BSO produced a major decrease in TW ( 203 + - 18 mg ) with respect to controls ( 365 + - 26 ) and a reduction in Hb content . - pubmed
The TETRAC group also showed marked reductions in TW ( 129 + - 15 ) and Hb concentration associated with a reduced VEGF content . - pubmed
BSO treatment increased protein carbonyl and tubulin abundance in comparison to controls . - pubmed
In conclusion , administration of BSO reduced the TW , which inversely correlated with protein carbonyl content , suggesting a loss of microtubule polymerization . - pubmed
Class I - specific histone deacetylase inhibitor MS-275 overrides TRAIL - resistance in melanoma cells by downregulating c - FLIP . - pubmed
Conversely , class II - selective HDACi MC1575 has shown no effect on the resistance of melanoma cells and was able exclusively to increase TRAIL - induced cell death in responsive cells . - pubmed
However , only MS-275 markedly decreased the expression levels of both the long and short c - FLIP isoforms . - pubmed
RNAi - mediated c - FLIP silencing resulted in caspase 8 - dependent apoptosis in survivor cells which was comparable to that observed following MS-275 treatment . - pubmed
Accordingly , enforced expression of ectopic c - FLIP has abolished the cooperative induction of apoptosis by the combination of MS-275 and TRAIL . - pubmed
Inhibition of class I HDAC isoenzymes 1 , 2 and 3 has resulted to be functionally important for c - FLIP downregulation by MS-275 . - pubmed
In contrast , knockdown of class II HDACs has had no effect on c - FLIP expression , thus explaining the dual incapacity of MC1575 to inhibit c - FLIP expression and sensitize cells resistant to TRAIL . - pubmed
The data reported here suggest that MS-275 represents a promising therapeutic approach in combination with TRAIL for treatment of cutaneous and uveal melanoma due to its ability to reduce c - FLIP expression . - pubmed
In the follow - up arm , a multivariable analysis adjusted for age , birth year , BMI , education , socioeconomic status , ethnicity , and physical activity yielded hazard ratios of 0.32 ( 95 % CI 0.10 - 0.82 , P = .002 ) for incident obesity , 0.49 ( 95 % CI 0.25 - 0.95 , P = .037 ) for incident triglycerides 150 mg / dL or greater , 0.56 ( 95 % CI 0.31 - 0.98 , P = .048 ) for low - density lipoprotein cholesterol 130 mg / dL or greater , and 2.14 ( 1.368 - 3.359 , P = .001 ) for high - density lipoprotein cholesterol less than 40 mg / dL for FMF participants compared with controls . - pubmed
Hesperetin Induces Apoptosis in Breast Carcinoma by Triggering Accumulation of ROS and Activation of ASK1 / JNK Pathway . - pubmed
The cytotoxic effect of hesperetin was due to the induction of apoptosis as evident from the phosphatidyl - serine externalization , DNA fragmentation , caspase-7 activation , and PARP cleavage . - pubmed
Pre - treatment with caspase-9 specific inhibitor ( Z - LEHD - fmk ) markedly attenuated apoptosis suggesting an involvement of intrinsic mitochondrial apoptotic cascade . - pubmed
Profiling of MAPKs revealed activation of JNK upon hesperetin treatment which was abrogated upon NAC pre - treatment . - pubmed
Additionally , inhibition of JNK by SP600125 significantly reversed hesperetin - mediated apoptosis . - pubmed
( + ) - Naloxone inhibits morphine - induced chemotaxis via prevention of heat shock protein 90 cleavage in microglia . - pubmed
RESULTS : Our results showed that 1muM morphine enhanced microglia activation and migration , decreased alpha - tubulin acetylation , and induced heat shock protein 90 ( HSP90 ) fragmentation and histone deacetylase 6 ( HDAC6 ) expression . - pubmed
Morphine - induced alpha - tubulin deacetylation and HSP90 fragmentation were HDAC6 - dependent . - pubmed
Pretreatment with ( + ) - naloxone ( 1nM ) inhibited morphine - evoked microglia activation and chemotaxis and prevented alpha - tubulin deacetylation and HSP90 fragmentation by inhibiting HDAC6 expression . - pubmed
CONCLUSION : Based on the findings of the present study , we suggest that ( + ) - naloxone inhibits morphine - induced microglia activation by regulating HDAC6 - dependent alpha - tubulin deacetylation and HSP90 fragmentation . - pubmed
A new type of bifunctional magnetic - fluorescent Fe3O4@SiO2 - PDI - PAA / Ca ( 2 + ) nanoparticles has been prepared by coating PDI - cored star polymers ( PDI - PAA ) onto the surface of Fe3O4@SiO2 core - shell nanostructures . - pubmed
RESULTS : The primary mechanism of action of colchicine is tubulin disruption . - pubmed
difficile total counts and spores , cytotoxin titres , short chain fatty acid concentrations , biometal concentrations , lactoferrin concentration and iron content of lactoferrin were monitored daily . - pubmed
difficile spores occurred 6 days post instillation of clindamycin , followed by rapid vegetative cell proliferation and detectable toxin production . - pubmed
difficile vegetative cell growth and toxin production , whereas apo - bLf and iron alone did not . - pubmed
Immunostaining of the muscle and axon markers F59 , znp1 , and zn5 revealed that 4 - MBC exposure leads to a disorganized pattern of slow muscle fibers and axon pathfinding errors during the innervation of both primary and secondary motor neurons . - pubmed
Hes3 is a component of the STAT3 - Ser / Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells . - pubmed
Here we provide initial observations implicating Hes3 in the cuprizone model of demyelination and remyelination . - pubmed
Based upon this approach , we identify a Janus kinase inhibitor and dbcAMP as powerful inducers of Hes3 gene expression . - pubmed
Spectro - microscopic data at the Fe L3 , La M5 , and Al K X - ray absorption edges reveal differing levels of deposited Fe on the ECAT catalysts corresponding with an overall loss in tetrahedral Al within the zeolite domains . - pubmed
Two small - molecule inhibitors of IGF-1R were chosen , NVP - ADW-742 ( IGF-1R - selective ) and OSI-906 ( a dual IGF-1R / insulin receptor alpha [ IR - alpha ] inhibitor ) . - pubmed
RESULTS : Novel mechanisms of resistance ( MOR ) emerged after dalotuzumab- , NVP - ADW-742- , and OSI-906 - based targeting of IGF-1R. - pubmed
MOR to dalotuzumab included upregulation of IRS1 , PI3 K , and STAT3 , as well as p38 MAPK , which was also induced by OSI-906 . - pubmed
pEIF4E(Ser209 ) , a key regulator of Cap - dependent translation , was induced in ridaforolimus - resistant ES cell lines . - pubmed
Unusual Horner ' s Syndrome in Recurrent Breast Cancer : Evaluation Using 18F - FDG PET / CT . - pubmed
18F - fluorodeoxyglucose ( FDG ) positron emission tomography / computed tomography ( PET / CT ) is a widely used imaging modality in the initial diagnosis of cancer , treatment response evaluation and detection of recurrence . - pubmed
Clinicians suspected Horner ' s syndrome , and the patient underwent FDG PET / CT for the evaluation of recurrence that could cause Horner ' s syndrome . - pubmed
FDG PET / CT demonstrated a focal hypermetabolic lesion in the right cervicothoracic junction area , corresponding to the preganglionic cervical sympathetic trunk . - pubmed
In this case , we could detect the exact location of the recurring lesion that caused Horner ' s syndrome using FDG PET / CT . - pubmed
Methotrexate combined with leflunomide exhibited a higher extension of CAL ( p=0.005 ) , and anti - TNF - alpha therapy with methotrexate was associated with a lower extension of CAL ( p=0.05 ) . - pubmed
CRITICAL ISSUES : The molecular mechanism whereby PXDN uses HOBr as a reactive intermediate to cross - link collagen IV , yet avoid collateral damage to nearby BM proteins , remains unclear . - pubmed
As seen with the sulfilimine cross - link of collagen IV , hypohalous acid oxidative events may be beneficial in select situations rather than uniformly deleterious . - pubmed
A large amount of recombinant SEA ( rSEA ) was obtained by engineering technology and was purified by Ni affinity chromatography column , and the expression and purity of rSEA and SEA were analyzed by SDS - PAGE . - pubmed
The result showed that large amount of SEs expressed within a short time at 28 C or thereabouts , and there was no impurity bands in electrophorogram after rSEA was purified by Ni affinity chromatography column . - pubmed
The result showed that the content of formic acid in mobile phase was an important factor for ionization of SEs in ESI - TOF . - pubmed
[ SEs + nH+ + mNH4 + ] n+m+ was shown on ESI - TOF spectra when SEA was detected by ESI - TOF in positive ion mode , and the numerical value of n+m was less than or equal to the number of basic amino acids in SEs . - pubmed
aeruginosa G6PDH is also a key enzyme in the metabolism of various carbon sources , such as glucose , glycerol , fructose , and mannitol . - pubmed
This potentially enabled Nec-1 to prevent SA - AKI . - pubmed
A C6 semi - laminectomy was performed in the control group , whereas a water - absorbent polyurethane polymer was implanted into the C6 epidural space in the compression group . - pubmed
CD73 facilitates tumor growth by upregulation of the adenosine ( immunosuppressive factor ) in the tumor microenvironment , however , its precise molecular mechanisms is not precisely understood . - pubmed
We used chitosan lactate ( ChLa ) nanoparticles ( NPs ) to deliver CD73 - specific small interfering RNA ( siRNA ) into cancer cells . - pubmed
alpha - Adrenergic stimulation with phenylephrine caused similar dose - dependent increases in resistance and pressure across salinities , indicating unaltered alpha - adrenoceptor sensitivity . - pubmed
Proteomic quantification identified the modulation of an important metabolic regulator , Fumarase , and concomitant accumulation of the metabolite fumarate . - pubmed
Two Coselected Distal Mutations in HIV-1 Reverse Transcriptase ( RT ) Alter Susceptibility to Nonnucleoside RT Inhibitors and Nucleoside Analogs . - pubmed
In contrast , both mutations affected the ability of RT to incorporate nucleoside analogs . - pubmed
A series of novel BN tetraphene derivatives have been prepared successfully for the first time via a post - functionalization strategy . - pubmed
ANCR expression and intracellular calcium ions were detected by quantitative real - time polymerase chain reaction ( qRT - PCR ) and laser confocal microscopy , respectively . - pubmed
Alkaline phosphatase ( ALP ) activity and calcium nodules were examined by ALP and alizarin red staining assay , respectively . - pubmed
Si - ANCR significantly increased the proliferation , ALP activity , calcium deposition of osteoblast cells and decreased apoptosis . - pubmed
The aglycon is generated by megaenzyme polyketide synthases ( PKSs ) from diverse acyl - CoA as precursor substrates . - pubmed
In this review , we have discussed versatile approaches for generating a wide range of macrolide structures by engineering the PKS and post - PKS cascades at either enzyme or cellular level in actinobacteria species , either the native or heterologous producer strains . - pubmed
Here we use hydrogen / deuterium exchange mass spectrometry , nuclear magnetic resonance ( NMR ) , and evolutionary sequence comparisons to map where and how the PHTH domain affects the Bruton ' s tyrosine kinase ( BTK ) domain . - pubmed
Through statistical comparisons of diverse tyrosine kinase sequences , we identify residues unique to BTK that coincide with the experimentally determined PHTH - binding surface on the kinase domain . - pubmed
All of the data suggested CDE possessed potential anti - osteoporotic activity and this effect was , at least in part , involved in modulation of RANKL / RANK / TRAF6 - mediated NF - kappaB and PI3K / AKT signaling as well as c - Fos and NFAT2 levels . - pubmed
The optimal extractions of TPC and AA were explored by varying the key variables , including the extraction temperature , ethanol concentration , extraction time , and ultrasonic power , through statistical optimization . - pubmed
In the variation experiment , the maximum TPC and AA values of 9.8 mg GAE / g and 91.8 % were obtained respectively with an extraction temperature of 74.4 C , ethanol concentration of 55.4 % , extraction time of 59.6 min , and ultrasonic power of 700 W. HPLC coupled with diode array detection was used to identify and quantify the phenolic compounds in the extracts , and EGCG ( 0.12 mg / g DM ) was identified as a major peak in the analysis , demonstrating that high value - added material with a bioactive property can be produced from LEM . - pubmed
LEM has a high TPC value , particularly with regard to EGCG , and excellent AA , considering it is highly used as a functional material for food , cosmetics , and medicine . - pubmed
There was increased RPE - related fb - P3 amplitude for those in the high alcohol use group . - pubmed
We found that high alcohol use participants had attenuated FRN amplitude in contrast with low alcohol use participants for both positive and negative RPEs but enhanced fb - P3 for both positive and negative RPE . - pubmed
To clarify the strength and underlying mechanism of amino acid binding to pyrite surfaces , we measured the unbinding ( pull - off ) force of ten amino acids and two oligo - peptides from water - pyrite interfaces using atomic force microscopy ( AFM ) . - pubmed
had negative correlations with lysophospholipids metabolites , and three of them had also negative correlations with serum IL-17alpha . - pubmed
We review two phase III trials of rituximab , a chimeric CD20 monoclonal antibody , used for the treatment of pemphigus vulgaris ( PV ) . - pubmed
This behavior was relatively accorded with the average oxidation state ( AOS ) results , evidencing the potential of EF process in enhancing the biodegradability of CFPZ . - pubmed
Analysis of 37 - S RNA , the common precursor to 17 - S , 5.8 - S and 26 - S rRNA and most probably the primary ribosomal transcript , shows that this RNA molecule contains already most if not all of the 36 - 37 pseudouridine residues found in the mature rRNAs . - pubmed
The pseudouridine residues within the rRNA sequences are apparently clustered to a certain degree as can inferred from the occurrence of a single oligonucleotide containing 3 pseudouridines , which was obtained by digestion of 26 - S rRNA with ribonuclease T1 . - pubmed
Porcine pancreatic elastase ( EC 3.4.21.11 ) has been immobilized on polyacrylamide beads using glutaraldehyde ad bridging reagent without important loss of catalytic activity . - pubmed
A nitroxide spin label , 1 - oxyl-2,2,5,5 - tetramethyl-4 - piperidinyl - ethylphosphonofluoridate , reacting covalently with the serine-195 residue of the active centre of free elastase was used as a conformational and dynamical electron spin resonance probe . - pubmed
The charcoal - coil : a dialysis - dialysate regeneration system . - pubmed
Fatty acids of medium chain length ( C6 to CII ) at 0 - 4 % produced a decrease in cell concentration , nonanoic and decanoic acids being the most effective . - pubmed
The gross morphology was revealed by NADH - diaphorase histochemistry , and the shape of the neurons by silver impregnation . - pubmed
Three morphologically distinct cell types were revealed by silver impregnation : mainly type I and type II neurons cells were seen in the duodenum , while the rest of the intestine contained type II and III cells . - pubmed
Gamma - aminobutyric acid showed non - uniform distribution in the two parts of the midgut : a stronger and more regular fibre staining was found in the duodenum then in the rest of the intestine . - pubmed
Following treatment of ROS cells with Pb2 + , intracellular levels of the calcium - binding protein osteonectin / SPARC were increased . - pubmed
These studies suggest that processes associated with osteonectin / SPARC translation and secretion are sensitive to Pb2 + . - pubmed
266 , 16633 - 16635 ) and the increased water permeability in oocytes that express mRNA encoding a 28 - kDa erythrocyte protein ( CHIP28 , Preston , B. M. , Carroll , T. P. , Guggino , W. B. , and Agre , P. ( 1992 ) Science 256 , 385 - 387 ) . - pubmed
Pf in proteoliposomes reconstituted with solubilized CHIP28 was high ( Pf = 0.03 cm / s , 37 degrees C ) , the activation energy was low ( 2.2 kcal / mol ) , and Pf was decreased by greater than 50 - fold by mercurial sulfhydryl reagents and Me2SO . - pubmed
The positive inotropic and chronotropic actions of parathyroid hormone ( PTH ) in cardiac cells are considered to be related to the modulation of calcium influx . - pubmed
The active synthetic fragment of bovine PTH , bPTH -( 1 - 34 ) , at a concentration of 1 microM , significantly enhanced the magnitude of L - channel currents by 67.8 % ( n = 13 , p less than 0.01 ) . - pubmed
The suppression or amplification of PTH - induced enhancement of inward currents by nifedipine , 1 microM , or Bay K-8644 , 5 microM , respectively , further indicated that the effect of PTH was specific for L - type calcium channels . - pubmed
However , bPTH -( 1 - 34 ) failed to modulate the magnitude or kinetics of T - channel currents . - pubmed
Ion replacement experiments and sensitivity to furosemide or a Cl - channel blocker indicated that PLC induced Cl- secretion . - pubmed
The effect of PLC was blocked by the protein kinase inhibitor , staurosporine . - pubmed
Carbachol , when added during the second phase of the PLC response , induced a ' paradox ' change in Isc : a rapid , transient increase in Isc was followed by a long - lasting decrease . - pubmed
This inhibition of the PLC response was more pronounced after elevation of the external Ca2 + concentration . - pubmed
A Ca2 + ionophore , ionomycin , and a Ca2 + channel activator , BAY K 8644 , also inhibited the PLC response . - pubmed
The results suggest dual dependence of the action of PLC on the intracellular Ca2 + concentration . - pubmed
In the plantaris , triglyceride content was unchanged , but citrate synthase activity in H ( 210 + /- 13 mumol min-1 g-1 ) was significantly greater than in SA ( 177 + /- 10 ) . - pubmed
According to those results , the superior sagittal sinus was supposed to have the incomplete occlusion or delayed blood flow that were not observed by DSA , MRI and CT scan performed previously . - pubmed
Those occlusive change in the superior sagittal sinus impeded the CSF absorption and elevated the pressure of venous inflow , then the arterio - venous communication has been developed. - pubmed
The following parameters were determined : ( 1 ) involution and damage of the endothelial cells by scanning and transmission electron microscopy ; ( 2 ) incidence and weight of thrombi in the lumens of the veins after 2 h stasis , and ( 3 ) effects of heparin on APTT and anti - Xa activity . - pubmed
Previous investigations have shown that the catalytic efficiency ( kcat / KM ) of pyruvate oxidase can be enhanced 450 - fold by chymotryptic cleavage of a 23 - residue peptide ( alpha - peptide ) from the carboxy terminus of the enzyme . - pubmed
The extent of carboxypeptidase hydrolysis was followed by analyzing the release of amino acids and by mass spectral analysis of the truncated alpha - peptides which were derived from the carboxypeptidase - treated preparations . - pubmed
The results indicate that the removal of 7 carboxy - terminal residues does not activate the enzyme whereas the removal of 10 or 11 residues produces activated pyruvate oxidase . - pubmed
Endoproteinase Glu - C specificity predicts hydrolytic cleavage of the peptide bond between Glu-561 and Val-562 with the removal of 11 residues from the carboxy terminus of the enzyme . - pubmed
Reaction of certain flavoproteins , the NADH : oxidoreductases , with different quinones is well known to form hydrogen - peroxide . - pubmed
Different redox cycling compounds are shown to be active in this " diaphorase " reaction by lens protein extract ( LCE ): Oxygen consumption can be detected in the presence of pyrroloquinoline quinone and juglone whereas 1,4 - naphthoquinone , menadione and paraquat are no redox cyclists in this flavoprotein catalyzed reaction . - pubmed
This excitation was associated with blockade of IPSPs , represented by positive waves , at a presynaptic site , which was antagonised by PDAc suggesting the involvement of PKC . - pubmed
4 ) Sphingosine , a dual PKC / calcium - calmodulin - dependent kinase inhibitor , did not reproduce H-7 - induced responses . - pubmed
In conclusion , in the hippocampus , PKC can modulate the IPSPs , the anomalous rectification , and the membrane potential , but PKC is unlikely to be the major intracellular mediator of the excitatory actions of acetylcholine . - pubmed
Nucleotides are known to enhance cyclooxygenase product formation in several tissues and , in addition , are believed to function as cofactors for mammalian 5 - lipoxygenases . - pubmed
We found that the phosphorylated adenine derivatives ( ATP , ADP , and AMP ) increased the formation of 12 - hydroxyeicosatetraenoic acid ( 12 - HETE ) by 2 - fold without altering the formation of cyclooxygenase products . - pubmed
This suggests that the 12 - lipoxygenase enzyme of platelets can be regulated by adenine nucleotides . - pubmed
We next determined the portion of the nucleotide molecule responsible for the enhanced 12 - lipoxygenase activity of platelets . - pubmed
In conclusion , platelet 12 - lipoxygenase was stimulated by ATP , as is true for several mammalian 5 - lipoxygenases . - pubmed
However , cyclooxygenase product formation by platelets was not altered by nucleotide addition . - pubmed
These studies suggest that following in vivo injury or platelet aggregation , when local concentrations of nucleotides are high , platelet lipoxygenase activity may be stimulated . - pubmed
A single topical treatment with 12 - O - tetradecanoylphorbol-13 - acetate caused a rapid initial decrease in the epidermal transcript levels corresponding to the Mr 67,000 and 59,000 keratin subunits . - pubmed
In contrast , the transcript level for the Mr 55,000 subunit was increased substantially 12- 48 h after treatment , the Mr 50,000 subunit transcript increased to a lesser extent , and the Mr 60,000 subunit message was transiently decreased at 12 h but returned to the level of solvent - treated skin by 24 h. Single exposure to the incomplete tumor promoters 4 - O - methyl-12 - O - tetradecanoylphorbol-13 - acetate , the ionophore A23187 , and mezerein induced changes in keratin gene transcripts similar to those of 12 - O - tetradecanoylphorbol-13 - acetate . - pubmed
Six NHS , acting in CHF , have presently been identified : three of them induce vasoconstriction and sodium retention ( sympathetic nervous systems , renin - angiotensin - aldosterone system and arginine - vasopressine system ) ; the remaining three offset or balance the former ones , acting , therefore as " counterregulators " ( prostaglandins -- PGE2 and PGI2 - - , dopaminergic system and atrial natriuretic factor ) . - pubmed
The onset and offset of the plasma melatonin rhythm in DD after 3 weeks of the respective light treatments was assessed for 48 hr , immediately after transferring to DD . - pubmed
The cytochrome oxidase activities ( measured with ascorbate + phenazine methosulfate as substrates ) were also decreased , but this segment of the respiratory chain was not rate - limiting for succinate or NAD - linked substrate oxidation . - pubmed
Analyses of the steady - state reduction kinetics of the respiratory chain carriers revealed that the rate - limiting step of the impaired respiration with succinate or NAD - linked substrates lies between the c cytochromes and cytochrome oxidase . - pubmed
Glucose counterregulation , hypoglycemia , and intensive insulin therapy in diabetes mellitus . - pubmed
The enhanced presentation of ferritin conjugated to goat anti - IgG ( H + L ) or to anti - IgM was sensitive to radiation doses greater than 2000 R , and was effective at less than one - tenth the number of spleen cells , consistent with the predominance of B cells as antigen - presenting cells for this form of the antigen rather than macrophages and dendritic cells only . - pubmed
Effect of magnesium on production of toxic - shock - syndrome toxin-1 : a collaborative study . - pubmed
We performed a series of collaborative experiments to clarify the effect of Mg ++ on production of toxic - shock - syndrome toxin-1 ( TSST-1 ) in various culture media . - pubmed
[ Evaluation of photocoagulation of the posterior pole with argon laser in edematous diabetic retinopathy ] . - pubmed
Use of macrophage inhibition factor and mast - cell degranulation tests for diagnosis of cloxacillin - induced cholestasis . - pubmed
Cloxacillin - induced cholestasis was diagnosed with the help of the macrophage inhibition factor and mast - cell degranulation tests . - pubmed
We conclude that the abnormalities of the divalent cation and phosphate metabolism can not be accounted for in full by increased circulating PTH activity , and are predominantly due to an intrinsic renal abnormality . - pubmed
The sleep promoting effects of Factor S can not be duplicated by serotonin , 4 - OH - butyrate , butyrolactone , GABA ( gamma - amino butyric acid ) , glutamic acid or 3 ' , 5 '- cyclic AMP when these substances are added to control fluids in concentrations up to 10 times greater than those found in normal c.s.f.5 . - pubmed
Hemoglobin Kariya [ alpha 40 ( C5 ) Lys leads to Glu ] : a new hemoglobin variant with an increased oxygen affinity . - pubmed
This hemoglobin showed an increased oxygen affinity , decreased heme - heme interaction and a lowered 2,3 - DPG effect . - pubmed
An IgM lambda monoclonal human antibody recognizing a sialic acid determined antigen fully expressed on newborn erythrocytes . - pubmed
Papainization of erythrocytes enhanced markedly the susceptibility of the corresponding antigen not only to antibody binding but also to the action of neuraminidase , indicating that sialic acid is the immunodominant component of the cryptic antigen . - pubmed
Insulin - like partial effects of agmatine derivatives in adipocytes . - pubmed
In previous investigations , we described insulin - like effects of agmatine [ ( 4 - aminobutyl ) guanidine ] in vitro . - pubmed
In the present work we have examined whether these effects of agmatine can be enhanced by variation in chain length ( C3 and C5 forms ) and by alkylation . - pubmed
In our test system , insulin decreases adrenalin - induced lipolysis to 40 % of the control value ( 100 % ) . - pubmed
Some of the glycoprotein / sialic acid of this internal membrane system ( 30 % ) remains bound for a considerable length of time . - pubmed
( 5 ) 3H - NAm was a more specific marker of glycoprotein than 2H - NAG . - pubmed
The following radioactive precursors , either combined or separately , dissolved in 50 microliter of saline containing 15 % BSA , were injected into vitreous body : [ 2 - 3H ] glycerol ( 50 microCi ) , [ 1 - 14C ] palmitate ( 15 microCi ) , and [ 1 - 14C ] linoleate ( 7.5 microCi ) . - pubmed
In order to obtain detailed information about the efferent connections of these lateral cortex subdivisions , iontophoretic injections of the anterograde tracers Phaseolus vulgaris leucoagglutinin and biotinylated dextran were made in the various parts . - pubmed
Regulation of volume activated chloride channels by protein kinase C - mediated phosphorylation of P - glycoprotein . - pubmed
The multidrug resistance P - glycoprotein ( Pgp ) transports hydrophobic drugs out of cells and has been recently associated with volume - activated chloride channels . - pubmed
Replacement of these residues with glutamate , to mimic the effect of phosphorylation , mimicked the effects of PKC on channel activation . - pubmed
These results indicate that overexpression of Pgp confers PKC - regulation of endogenous volume - activated chloride channels . - pubmed
More generally they favour a model in which Pgp acts as a regulator of volume - activated chloride channels . - pubmed
Four treatment regimens were compared : radiation treatment alone ( I ) , combined radiation , drug and estrogen treatments ( II ) , estrogen therapy alone ( III ) , combined estrogens and radiation ( IV ) . - pubmed
NIR gene encodes a protein of 378 amino acid residues including a putative signal peptide of 37 residues . - pubmed
The DNA - derived amino acid sequence of NIR is in complete agreement with that from Edman degradation and the DNA coding sequence of BCP is also consistent with its partial N - terminal amino acid sequence . - pubmed
The correlation between the concentrations of myoglobin and malonic dialdehyde suggests that there is an association between hypermyoglobinemia and the status of cellular membranes . - pubmed
Propeptide and glutamate - containing substrates bound to the vitamin K - dependent carboxylase convert its vitamin K epoxidase function from an inactive to an active state . - pubmed
The vitamin K - dependent gamma - glutamyl carboxylase catalyzes the posttranslational conversion of glutamic acid to gamma - carboxyglutamic acid in precursor proteins containing the gamma - carboxylation recognition site ( gamma - CRS ) . - pubmed
We show that in the absence of gamma - CRS - containing propeptide and/or glutamate - containing substrate , carboxylase has little or no epoxidase activity . - pubmed
Epoxidase activity is induced by Phe - Leu - Glu - Glu - Leu ( FLEEL ) ( 9.2 pmol per min per pmol of enzyme ) , propeptide , residues -18 to -1 of proFactor IX ( 3.4 pmol per min per pmol of enzyme ) , FLEEL and propeptide ( 100 pmol per min per pmol of enzyme ) , and proPT28 ( HVFLAPQQARSLLQRVRRANTFLEEVRK , residues -18 to +10 of human acarboxy - proprothrombin ) , ( 5.3 pmol per min per pmol of enzyme ) . - pubmed
These results indicate that in the absence of propeptide or glutamate - containing substrate , oxygenation of vitamin K by the carboxylase does not occur . - pubmed
Upon addition of propeptide or glutamate - containing substrate , the enzyme is converted to an active epoxidase . - pubmed
X - ray diffraction data analysis shows that noncrystallographic symmetry - related arcelin-1 molecules form a lectin - like dimer and reveals the presence of a solvent - exposed anion binding site on the protein , at a crystal - packing interface . - pubmed
Transferrin conjugates of doxorubicin : synthesis , characterization , cellular uptake , and in vitro efficacy . - pubmed
Thus , thiolated human serum transferrin was conjugated with four maleimide derivatives of doxorubicin that differed in the stability of the chemical link between drug and spacer . - pubmed
Resonance raman characterization of the heme domain of soluble guanylate cyclase . - pubmed
We propose that the absence of hydrogen - bond formation between a distal residue and exogenous ligands is physiologically relevant in lowering the oxygen affinity of heterodimeric sGC and , therefore , stabilizing the ferrous , active form of the enzyme under aerobic conditions . - pubmed
High affinities of Ki = 0.81 and 2.6 nM for the [ 3H ]- cytisine rat brain nicotinic acetylcholine binding sites were found for the 5 - pyrimidinyl and the 5 -( 2 - amino )- pyrimidinyl epibatidine analogues , respectively . - pubmed
Superstructures and defect structures revealed by atomic - scale STM imaging of WO3(001 ) . - pubmed
Stretch - induced increases in growth were completely blocked on addition of anti - IGF - I and partially blocked with platelet - derived growth factor ( PDGF ) antibodies and with a tyrosine kinase inhibitor ( tyrphostin-1 ) . - pubmed
In the present study , we investigated whether serine phosphorylation of the SR Ca ( 2 +)- ATPase occurs in the intact functioning heart . - pubmed
Following 45 - 50 min of ( 32 ) P perfusion , the hearts were freeze - clamped , SR isolated , and analyzed for protein phosphorylation . - pubmed
SDS - polyacrylamide gel electrophoresis and autoradiography showed phosphorylation of several peptides including the Ca ( 2 +)- ATPase and Ca ( 2 + ) release channel ( ryanodine receptor ) . - pubmed
The Ca ( 2 +)- ATPase showed immunoreactivity with a phosphoserine monoclonal antibody indicating that the in situ phosphorylation occurred at the serine residue . - pubmed
Quantification of Ca ( 2 +)- ATPase phosphorylation in situ yielded a value of 208 + /- 12 pmol ( 32)P / mg SR protein which corresponded to the phosphorylation of approximately 20 % of the Ca ( 2 + ) pump units in the SR membrane . - pubmed
, in the absence of any inotropic intervention ) , a considerable steady - state pool of serine - phosphorylated Ca ( 2 +)- ATPase likely exists in the normally beating heart . - pubmed
These findings demonstrate that serine phosphorylation of the Ca ( 2 +)- ATPase is a physiological event which may be important in the regulation of SR function . - pubmed
At 6 to 24 h after H ( 2 ) O ( 2 ) injection , the level of LPO was significantly higher in the H ( 2 ) O ( 2 ) group than in the H ( 2 ) O ( 2 ) + pHGF - treated group . - pubmed
This finding indicated that pHGF protected against cell membrane damage in H2O2 - induced acute lung injury . - pubmed
In the H ( 2 ) O ( 2 ) + pHGF - treated group , TUNEL - positive signals were reduced compared with those in the H ( 2 ) O ( 2 ) group . - pubmed
In the H ( 2 ) O ( 2 ) + pHGF - treated group , the formation of subendothelial blebs was seen , but no severe subendothelial blebs were observed . - pubmed
These findings indicated that pHGF protects the progression of H ( 2 ) O ( 2 )-induced acute lung injury , and showed that pHGF acts to stabilize the cell membrane in capillary endothelial cells and lung epithelial cells . - pubmed
A series of cis-1 ( S ) 2 ( R )- amino-2 - indanol based compounds with a biphenylmethyl group at the P1 ' position was found to be potent aggrecanase inhibitors . - pubmed
Inhibition of estrone sulfatase ( ES ) by derivatives of 4 -[( aminosulfonyl ) oxy ] benzoic acid . - pubmed
In our search for potent inhibitors of the enzyme estrone sulfatase ( ES ) , we have undertaken the synthesis and biochemical evaluation of a range of straight chain alkyl esters of 4 -[( aminosulfonyl ) oxy ] benzoic acid . - pubmed
Gadodiamide and gadopentetate dimeglumine in MRI versus spiral CT in the diagnosis of liver lesions . - pubmed
In addition , the ratio of activated tyrosine phosphorylated STAT3 to total STAT3 was not significantly altered in failing hearts compared with non - failing controls . - pubmed
CpZPC , a newt ZPC molecule , localizes to the inner surface of the egg envelope . - pubmed
Two bands corresponding to 84 kDa and 70 kDa in the egg envelopes were detected by immunoblotting with an antiserum raised against a 9 amino acid peptide in the C - terminus domain of cpZPC . - pubmed
In all species studied to date , the function of heat shock protein 90 ( Hsp90 ) , a ubiquitous and evolutionarily conserved molecular chaperone , is inhibited selectively by the natural product drugs geldanamycin ( GA ) and radicicol . - pubmed
Mechanism of inhibition of Catalase by 3 - amino-1,2,4 - triazole . - pubmed
Both ( 1 ) H- and ( 31 ) P - MRS have revealed significant disturbances in amino acids , lipids , and phosphorus - containing metabolites within tumors . - pubmed
( 1 ) H - MRS has been successfully used to quantify the extent of neuronal cell loss imposed on the brain during radiotherapy . - pubmed
( 19 ) F - MRS opens access to artificially induced fluorocompounds such as 5 - fluorouracil and its metabolites . - pubmed
Acute renal failure was not observed in this dosage of oral nickel administration by analysing NAG , beta2 - microglobulin , urine albumin and urine protein . - pubmed
METHODS : Ischemia - reperfusion injury was studied in forearm skeletal muscle by technetium Tc 99m - labeled annexin A5 scintigraphy . - pubmed
Immediately on reperfusion , annexin A5 labeled with technetium Tc 99 m was administered intravenously , and ischemia - reperfusion injury was expressed as the percentage difference in radioactivity between the experimental arm and the control arm 1 and 4 hours after reperfusion . - pubmed
RESULTS : Dipyridamole treatment significantly reduced annexin A5 targeting in skeletal muscle compared with the control group ( thenar region , 13 % + /- 7 % versus 22 % + /- 15 % at 1 hour after reperfusion and 9 % + /- 6 % versus 27 % + /- 13 % at 4 hours for dipyridamole and control groups , respectively [ P = .01 ] ; flexor region , 4 % + /- 8 % versus 7 % + /- 6 % at 1 hour after reperfusion and 1 % + /- 4 % versus 10 % + /- 9 % at 4 hours for dipyridamole and control groups , respectively [ P = .01 ] ) . - pubmed
OBJECTIVES : Adding ribavirin to interferon improves treatment response for patients with chronic hepatitis C , but the effects of ribavirin monotherapy are unclear . - pubmed
Ribavirin had no significant effect on sustained ( risk difference ( RD ) , 0 % ; 95 % confidence intervals ( CI ) , -2 % to 3 % ) or end - of - treatment virological response ( RD , 0 % ; 95 % CI , -3 % to 3 % ) . - pubmed
Ribavirin had no significant effect on liver - related morbidity plus mortality ( RD , 0 % ; 95 % CI , -2 % to 3 % ) . - pubmed
Purified gamma - sm was used as antigen to screen the displayed phage hybrid antibody library rescued by helper phage M13K07 for three rounds . - pubmed
PURPOSE : Autosomal dominant Stargardt - like ( STGD3 ) disease results from mutations in the ELOVL4 gene ( elongation of very - long - chain fatty acids ) . - pubmed
Characterization included examining gene message and protein levels , electroretinogram ( ERG ) , retinal morphology and ultrastructure , and plasma and retinal fatty acid composition . - pubmed
In order to determine whether increase in global protein glutathionylation by HEDS leads to loss of function of an important protein , we compared the effect of HEDS on global protein glutathionylation with that of Ku protein function , a multifunctional DNA repair protein , using a novel ELISA . - pubmed
E89 cells treated with HEDS showed a significant loss of Ku protein binding to DNA . - pubmed
TRPM5 is known to be abundantly expressed in taste receptor cells where it participates in sweet , amino acid and bitter perception . - pubmed
In addition , a time course experiment was set up to determine how the transcription profile of TbICL , TbMLS and TbFBP in axenic - grown mycelia is affected by different carbon sources . - pubmed
The high induction of TbICL , TbMLS and TbFBP transcription and the translocation of lipids in fruiting bodies let us hypothesize that glyoxylate cycle and gluconeogenesis are key metabolic pathways in the recycling of existing cell material and the channelling towards the biosynthesis of new cell components during the maturation of fruiting bodies . - pubmed
Clavulanic acid and cephamycin C production are improved in the DeltaareB mutant although no drastic difference in ccaR expression was observed . - pubmed
The azoles are relatively safe , but clinicians should be aware of drug - drug interactions and adverse effects , including visual disturbances ( with voriconazole ) , elevations in liver transaminase levels , and skin rashes . - pubmed
There have been few detailed studies of binding characterizations for the complex formation by TNFalpha and clinically relevant antagonists , particularly Infliximab ( Remicade ) and Etanercept ( Enbrel ) . - pubmed
Bacterial and yeast two - hybrid experiments revealed a complex network of interactions involving TagA , - B , - E , - F , - G , - H , and - O and the cell shape determinants MreC and MreD ( encoded by the mreBCD operon and presumably involved in the spatial organization of PG synthesis ) . - pubmed
The treatment plan for this patient included the following medications : pioglitazone hydrochloride ( a thiazolidinedione , 30 mg / d ) ; irbesartan ( an angiotensin receptor blocker , 150 mg / d titrated to 300 mg / d ) ; hydrochlorothiazide ( an antikaliuretic agent , 12.5 mg / d ) ; and aspirin ( 325 mg / d ) . - pubmed
Sitagliptin phosphate ( a dipeptidyl peptidase IV inhibitor , 50 mg / d ) was added to the treatment regimen to improve glycemic control . - pubmed
Preliminary study of fibrin glue combined with pingyangmycin for the treatment of venous malformations in the oral and maxillofacial region . - pubmed
PURPOSE : To observe the outcome of using fibrin glue ( FG ) combined with Pingyangmycin ( Tianjin Taihe Pharmaceutical Co Ltd , Tianjin , China ) in the treatment of venous malformations ( VMs ) in the oral and maxillofacial region . - pubmed
In all of these cells , K channel activity , reflected in the net , steady - state outward K + currents ( IK ) , was inversely related to the plasma membrane PtdInsP2 level . - pubmed
Consistent with this , short - term manipulations decreasing PtdInsP2 levels in the High PIs , such as pretreatment with the phytohormone abscisic acid ( 25 microM ) or neutralizing the bath solution from pH 5.6 to pH 7 , increased IK ( i.e. - pubmed
These results are consistent with NtORK inhibition by the negatively charged PtdInsP2 in the internal plasma membrane leaflet . - pubmed
Polymerase chain reaction of a urate anion exchanger known to regulate blood urate level ( SLC22A12 gene : URAT1 ) demonstrated that homozygous mutations in exon 4 ( W258X ) . - pubmed
Two hundred and forty fish ( average weight : 27.01 g / fish ) , distributed equally into 4 different groups ( control , T(1 ) , T(2 ) , and T(3 ) ) , each with 6 replicates were fed with control ( 40 % crude protein , CP and 0.1 % vitamin C ) , T(1 ) ( 40 % CP and 0.1 % vitamin C ) , T ( 2 ) ( 50 % CP and 0.1 % vitamin C ) , and T ( 3 ) ( 50 % CP and 0.2 % vitamin C ) diets for 90 days . - pubmed
Results indicated significant reduction in the growth performance , survival , and activities of lactate dehydrogenase ( liver and muscle ) , malate dehydrogenase ( liver and muscle ) , enzymes of protein metabolism ( aspartate amino transferase in liver and alanine amino transferase in liver and muscle ) , acetyl choline esterase ( brain ) , alkaline phosphatase activity ( liver ) , and ATPase ( gill ) enzymes of group fed control diet and exposed to endosulfan . - pubmed
Further , high CP and vitamin C diet reduced endosulfan accumulation in the muscle . - pubmed
Each side was randomly assigned to the control group ( CG : bioabsorbable membrane made of glycolide and lactide copolymer ) or the test group ( TG : ADM as a membrane ) . - pubmed
These include nucleoside and nonnucleoside reverse transcriptase inhibitors , protease and integrase strand transfer inhibitors , CCR5 antagonists , and an entirely new class of maturation inhibitors . - pubmed
Neutralization of IL-17 suppressed the hepatotoxic effect of halothane . - pubmed
In conclusion , we demonstrated that IL-17 is involved in the halothane - induced liver injury . - pubmed
The maximum burning velocity of 1234yf was found to be 1.2+/-0.3 cm s(-1 ) , which was approximately one - fifth that of HFC-32 ( 6.7 cm s(-1 ) ) and one order of magnitude less than those of 1243yf ( 19.8 cm s(-1 ) ) and 1243zf ( 14.1 cm s(-1 ) ) . - pubmed
Prediction of asthma in symptomatic preschool children using exhaled nitric oxide , Rint and specific IgE. - pubmed
Associations of FE(NO ) ( n=308 ) , Rint ( n=482 ) and specific IgE ( n=380 ) at 4 years with wheezing and asthma at the ages of 5 - 8 years were assessed using repeated measurement analyses . - pubmed
RESULTS : FE(NO ) and specific IgE measured at 4 years were associated with wheezing and asthma at 8 years . - pubmed
Both tests also remained significant predictors after mutual adjustment and adjustment for clinical history : OR on wheezing at 8 years for FE(NO ) ( ( 10)log - scale , per IQR ) 1.6 ( 95 % CI 1.1 to 2.2 ) and for specific IgE 2.8 ( 95 % CI 1.9 to 4.1 ) . - pubmed
CONCLUSIONS : In preschool children with symptoms suggestive of asthma , both FE(NO ) and specific IgE measured at age 4 , but not Rint , improved the prediction of asthma symptoms until the age of 8 years , independent of clinical history . - pubmed
Expression profiles of genes in CRPC support a role for the transcriptional activity of the androgen receptor ( CCNH , CUEDC2 , FLNA , PSMA7 ) , steroid synthesis and metabolism ( DHCR24 , DHRS7 , ELOVL5 , HSD17B4 , OPRK1 ) , neuroendocrine ( ENO2 , MAOA , OPRK1 , S100A10 , TRPM8 ) , and proliferation ( GAS5 , GNB2L1 , MT - ND3 , NKX3 - 1 , PCGEM1 , PTGFR , STEAP1 , TMEM30A ) , but neither supported nor discounted a role for cell survival genes . - pubmed
Characterization of nicotinamidases : steady state kinetic parameters , classwide inhibition by nicotinaldehydes , and catalytic mechanism . - pubmed
Nicotinamidases are metabolic enzymes that hydrolyze nicotinamide to nicotinic acid . - pubmed
Nicotinaldehyde was determined to be a potent competitive inhibitor of these enzymes , binding in the low micromolar to low nanomolar range for all nicotinamidases tested . - pubmed
Inhibitions are consistent with reaction of the universally conserved catalytic Cys on each enzyme with the aldehyde carbonyl carbon to form a thiohemiacetal complex that is stabilized by a conserved oxyanion hole . - pubmed
pneumoniae nicotinamidase can catalyze exchange of ( 18 ) O into the carboxy oxygens of nicotinic acid with H ( 2 )( 18 ) O. - pubmed
Disease activity , lipid profile , serum malondialdehyde ( MDA ) , tumor necrosis factor - alpha ( TNF - alpha ) , resistin , adiponectin , and brachial artery flow - mediated dilation ( FMD ) were measured before and after 6 months of treatment . - pubmed
RESULTS : Atorvastatin combined with MTX therapy significantly reduced serum total cholesterol , low - density lipoprotein cholesterol , and triglycerides , and increased high - density lipoprotein cholesterol ( p & lt ; 0.001 ) . - pubmed
Disease activity variables , serum MDA , TNF - alpha , resistin , adiponectin , and FMD were significantly improved by the drug combinations ( p & lt ; 0.001 ) . - pubmed
In the present study , we determined whether a cyclophosphamide - induced reduction of naturally occurring regulatory T cells ( Tregs ) was associated with the anti - tumor activity in our nonmyeloablative SCT system . - pubmed
These results indicated that cyclophosphamide - induced reduction of recipient Tregs is associated with retardation of tumor progression via the expansion of host - reactive donor T cells and IFN - gamma production after DLI in our nonmyeloablative SCT system . - pubmed
Inhibition of the MDM2 - p53 interaction using either Nutlin-3 or MDM2 RNA interference resulted in hyperactivation of the p53 pathway and a strong induction of apoptosis in cisplatin - sensitive and -resistant TC cells . - pubmed
Suppression of wild - type p53 induced resistance to Nutlin-3 in TC cells , demonstrating the key role of p53 for Nutlin-3 sensitivity . - pubmed
More specifically , our results indicate that p53 - dependent induction of Fas membrane expression ( ~threefold ) and enhanced Fas / FasL interactions at the cell surface are important mechanisms of Nutlin-3 - induced apoptosis in TC cells . - pubmed
Importantly , an analogous Fas - dependent mechanism of apoptosis upon Nutlin-3 treatment is executed in wild - type p53 expressing Hodgkin lymphoma and acute myeloid leukaemia cell lines . - pubmed
Finally , we demonstrate that Nutlin-3 strongly augmented cisplatin - induced apoptosis and cell kill via the Fas death receptor pathway . - pubmed
ROS was determined using the reactive dye , Dichlorodihydrofluorescin ( DCFH ) , and the Plasmid Scission Assay ( PSA ) , which determine DNA damage . - pubmed
These " pleiotropic " effects stem from their inhibition of prenylation of the small GTP - binding proteins Ras and Rho , and to the disruption , or depletion , of cholesterol rich membrane micro - domains ( membrane rafts ) . - pubmed
The inhibition of mevalonate pathway by statins impairs modification of Ras and Rho GTPases , which play key roles in signaling pathways related to tumor formation , metastasis and cell death . - pubmed
In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired . - pubmed
We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center . - pubmed
Photoelectron spectroscopy and computational modeling of thymidine homodimer anions . - pubmed
The intact thymidine homodimer anion ( dT(2)(- ) ) was generated in the gas phase using an infrared desorption / photoemission source and recorded by a pulsed photoelectron spectrometer . - pubmed
Here we report that the most stable anion radical homodimer geometries observed in the PES are the anionic nucleoside coordinated by the O8 atom of thymine to the deoxyribose of the second neutral nucleoside . - pubmed
RUT - positive patients without a post - treatment evaluation were invited for a urea breath test . - pubmed
In vitro cytocompatibility evaluation of MGF - Ct24E chemically grafted and physically blended with maleic anhydride modified poly ( D , L - lactic acid ) . - pubmed
As a growth repair factor , mechano - growth factor ( MGF ) and its 24 amino acid peptide analogs corresponding to the unique C - terminal E - domain ( MGF - Ct24E ) positively regulate bone regeneration . - pubmed
MGF - Ct24E was introduced into the poly ( D , L - lactic acid ) ( PDLLA ) to improve bone regeneration in our previous study . - pubmed
MGF - Ct24E - grafted PDLLA was chemically characterized and showed potential as a biofunctional polymer . - pubmed
In this study , we evaluated the cytocompatibility of MGF - Ct24E chemically grafted and physically blended with maleic anhydride modified PDLLA , relative to maleic anhydride modified PDLLA ( MPLA ) as the control . - pubmed
Electro - assisted solid - phase microextraction based on poly ( 3,4 - ethylenedioxythiophen ) combined with GC for the quantification of tricyclic antidepressants . - pubmed
In this study , a platinum wire coated with poly ( 3,4 - ethylenedioxythiophen ) was used as an electro - assisted solid - phase microextraction fiber for the quantification of tricyclic antidepressant drugs in biological samples by coupling to GC employing a flame ionization detector . - pubmed
Group I metabotropic glutamate receptors in the medial prefrontal cortex : role in mesocorticolimbic glutamate release in cocaine sensitization . - pubmed
One , 7 , or 21 days from the fourth injection , dual - probe microdialysis experiments were performed wherein Group I metabotropic glutamate receptor agonist DHPG was infused into the medial prefrontal cortex and glutamate levels in this region as well as the nucleus accumbens or ventral tegmental area were examined . - pubmed
The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis , cell survival and/or proliferation , increase in intracellular calcium , and gene transcription . - pubmed
Extensive laboratory workup revealed low levels of serum cortisol ( and its precursors 17alpha - hydroxyprogesterone and 11 - deoxycortisol ) , adrenal androgens ( dehydroepiandrosterone sulfate and androstenedione ) , and estrogen ( estradiol ) ; and high levels of mineralocorticoids precursors ( 11 - deoxycorticosterone and corticosterone ) with high levels of ACTH , FSH and LH . - pubmed
Of the 62 rifampin - monoresistant samples by LPA , 38 ( 61.4 % ) showed rifampin resistance , while 21 ( 33.8 % ) were found susceptible to rifampin by Xpert MTB / RIF using cartridge version G4 . - pubmed
Of the 116 pan - susceptible samples , only 83 were available for Xpert MTB / RIF testing ; 4 ( 5.1 % ) were rifampin resistant , 74 ( 94.8 % ) were susceptible , and 5 ( 6.0 % ) showed an error . - pubmed
The MGIT960 results showed 100 % agreement with LPA results but only 64.4 % agreement with Xpert MTB / RIF results . - pubmed
Sequencing analysis of discrepant samples showed 91.3 % concordance with LPA but only 8.7 % concordance with the Xpert MTB / RIF assay . - pubmed
Molecular mechanisms of methoctramine binding and selectivity at muscarinic acetylcholine receptors . - pubmed
Methoctramine ( N , N '- bis [ 6 -[[( 2 - methoxyphenyl )- methyl ] hexyl ]- 1,8 - octane ] diamine ) is an M(2)-selective competitive antagonist of muscarinic acetylcholine receptors and exhibits allosteric properties at high concentrations . - pubmed
To reveal the molecular mechanisms of methoctramine binding and selectivity we took advantage of reciprocal mutations of the M ( 2 ) and M ( 3 ) receptors in the second and third extracellular loops that are involved in the binding of allosteric ligands . - pubmed
To this end we performed measurements of kinetics of the radiolabeled antagonists N - methylscopolamine ( NMS ) in the presence of methoctramine and its precursors , fluorescence energy transfer between green fluorescent protein - fused receptors and an Alexa-555 - conjugated precursor of methoctramine , and simulation of molecular dynamics of methoctramine association with the receptor . - pubmed
We confirm the hypothesis that methoctramine high - affinity binding to the M ( 2 ) receptors involves simultaneous interaction with both the orthosteric binding site and the allosteric binding site located between the second and third extracellular loops . - pubmed
Methoctramine can bind solely with low affinity to the allosteric binding site on the extracellular domain of NMS - occupied M ( 2 ) receptors by interacting primarily with glutamate 175 in the second extracellular loop . - pubmed
In this mode , methoctramine physically prevents dissociation of NMS from the orthosteric binding site . - pubmed
Our results also demonstrate that lysine 523 in the third extracellular loop of the M ( 3 ) receptors forms a hydrogen bond with glutamate 219 of the second extracellular loop that hinders methoctramine binding to the allosteric site at this receptor subtype . - pubmed
Impaired interaction with the allosteric binding site manifests as low - affinity binding of methoctramine at the M ( 3 ) receptor . - pubmed
The level of H2S in serum , the activity of superoxide dismutase ( SOD ) and the content of malondialdehyde ( MDA ) , as well as anti - superoxide anions in vascular tissue were determined by spectrophotometry . - pubmed
Angiotensin II ( AngII ) levels in plasma were measured using competitive enzyme immunoassay . - pubmed
Decreased vascular endothelial exfoliative cells and vascular smooth muscle cell ( SMC ) proliferation were shown in NaHS groups by HE staining . - pubmed
Masson staining analysis showed reduced relative contents of collagen fibers ( P & lt ; 0.05 ) and SMC ( P & lt ; 0.05 ) in NaHS groups . - pubmed
In the D - gal group , the concentration of AngII in plasma was significantly increased compared with that in vehicle group , while it was decreased in NaHS groups ( P & lt ; 0.05 ) . - pubmed
Moreover , levels of vascular tissue anti - superoxide anion and the activity of SOD were obviously higher , MDA was significantly lower in all NaHS treated groups than those in the D - gal group respectively ( P & lt ; 0.05 ) . - pubmed
Western blot analysis showed that the expression of AT1R was increased in D - gal group compared with that in vehicle group , while it was decreased after treatment with NaHS compared with that in D - gal group ( P & lt ; 0.05 ) . - pubmed
These results suggest that exogenous H2S can ameliorate the age - related changes of aortic morphology , decrease the concentration of AngII in plasma , down - regulate the expression of AT1R in vascular tissue , and mitigate the level of oxidative stress . - pubmed
Evaluation of the Xpert MTB / RIF assay for diagnosis of tuberculosis and rifampin resistance in county - level laboratories in Hunan province , China . - pubmed
For the detection of rifampin resistance , the sensitivity of MTB / RIF RIF - resistance detection was 92.9 % , and the specificity was 98.7 % . - pubmed
Of the 26 Xpert MTB / RIF - positive and RIF - resistant patients confirmed by LJ proportion tests , 20 ( 76.9 % ) patients were infected by MDR - TB . - pubmed
We found higher levels of 8 - OHdG and PC in the NTD group than in the control group ; however , we did not observe a statistically significant difference in 8 - iso - PGF2alpha levels between the NTD and the control groups . - pubmed
NTD risk increased with increasing quartiles of 8 - OHdG [ odds ratio ( OR)=1.17 ; 95 % confidence interval ( CI ) 0.39 - 3.51 ; OR=2.19 ; 95 % CI , 0.68 - 7.01 ; OR=3.70 ; 95 % CI , 1.30 - 10.51 , for the second , third , and fourth quartile relative to the lowest quartile , respectively ; P=0.009 ] , and with increasing quartiles of PC ( OR=2.26 ; 95 % CI , 0.66 - 7.69 ; OR=3.86 ; 95 % CI , 1.17 - 12.80 ; OR=5.98 ; 95 % CI , 1.82 - 19.66 , for the second , third , and fourth quartile relative to the lowest quartile , respectively ; P=0.002 ] . - pubmed
As expected , TNF - alpha increased ET-1 and ETA expression in a dose - dependent manner ; furthermore , application of the TNF - alpha inhibitor CAY10500 partially inhibited hypoxia - induced ET-1 and ETA expression . - pubmed
Expression of STAT1 was inhibited by administration of 50muM fludarabine ( 2 - fluoro - ara - AMP ) for 2 , 4 , 8 , 24 , 48 or 72h or by using small interfering RNA ( siRNA ) . - pubmed
Fludarabine inhibits the so induced phosphorylation of STAT1 when administered 48 or 72h before stimulation . - pubmed
Fludarabine reduces STAT1 DNA - binding capacity . - pubmed
Suppression of STAT1 by fludarabine or siRNA influences activity of ERK1 / 2 which indicates a crosstalk between both pathways . - pubmed
In this study , quantitative real - time polymerase chain reaction analyses revealed that venom allergen 5 was significantly overexpressed in adult females of both deltamethrin - resistant laboratory populations and two field populations . - pubmed
The overexpression of venom allergen 5 decreased the susceptibility of mosquito cells to deltamethrin , while knockdown of this gene by RNAi increased the susceptibility of mosquitoes to deltamethrin . - pubmed
Treating larval or adult worms with antifungal azole CYP450 inhibitors results in worm death at low micromolar concentrations . - pubmed
In addition , combinations of SmCYP450 - specific dsRNA and miconazole show additive schistosomicidal effects supporting the hypothesis that SmCYP450 is the target of miconazole . - pubmed
LPO , SOD , CAT and GST levels were significantly higher in liver and kidney , whereas GSH levels declined significantly compared to fish from the reference site . - pubmed
For volatile profile , a total of 29 volatile compounds were identified by HP - SPME - GC - MS , mainly alcohols , esters , aldehydes , ketones and phenols . - pubmed
Solution Structure and Constrained Molecular Dynamics Study of Vitamin B12 Conjugates of the Anorectic Peptide PYY(3 - 36 ) . - pubmed
Vitamin B12 -peptide conjugates have considerable therapeutic potential through improved pharmacokinetic and/or pharmacodynamic properties imparted on the peptide upon covalent attachment to vitamin B12 ( B12 ) . - pubmed
Chronic estradiol treatment did not affect ovarian blood flow at rest , while changed the basal ovarian estradiol secretion rate , i.e. - pubmed
RESULTS : MSG and MSH groups had elevated blood pressure , triglyceride , waist circumference and fasting glucose values in comparison to gingivitis and healthy groups ( P<0.0001 ) . - pubmed
Amino acids desmosine and isodesmosine are unique cross - links in mature elastin fibers and can serve as biomarkers of elastin degradation when measured in body fluids . - pubmed
Synthesis and pre - clinical evaluation of a new class of high - affinity 18F - labeled PSMA ligands for detection of prostate cancer by PET imaging . - pubmed
PURPOSE : Current clinical imaging of PSMA - positive prostate cancer by positron emission tomography ( PET ) mainly features 68Ga - labeled tracers , notably [ 68Ga ] Ga - PSMA - HBED - CC . - pubmed
We aimed to develop high - affinity PSMA inhibitors labeled with fluorine-18 as alternative tracers for prostate cancer . - pubmed
, and co - administration of PMPA was used to determine whether uptake was PSMA - specific . - pubmed
The synthesized bioavailable curcuminoid formulation Cureit was characterizations by Nuclear magnetic resonance spectroscopy ( NMR ) , scanning electron microscopy ( SEM ) , X - ray diffraction ( XRD ) , Fourier transform infra - red ( IR ) , current - voltage ( I - V ) study , Quadrupole Time - of - Flight Mass Spectrometry ( Q - TOF ) , differential scanning calorimeter ( DSC ) and thermogravimetric analysis ( TGA ) . - pubmed
The chemical profile of Cureit was analyzed by Q - TOF confirmed the presence of curcuminoids ( curcumin , demethoxycurcumin and bismethoxycurcumin ) , lactones , sesquiterpenes and their derivatives derived from polar layer , aromatic turmerone , dihydroturmerone , turmeronol , curdione and bisacurone derived from non - polar layer . - pubmed
IR , XRD , DSC and TGA also confirmed the presence of curcuminoids with high stability in the PNS formulation . - pubmed
In Wilson disease ( WD ) defective AT7B function leads to biliary copper excretion and pathologic copper accumulation , particularly in liver and brain , where it induces cellular damage . - pubmed
Mechanistically , chronic alcohol consumption decreased the number of antitumor CD8 + T cells but increased the number of tumor - associated macrophages ( TAMs ) in the liver in DEN - initiated tumorigenesis . - pubmed
Moreover , chronic alcohol consumption aggravated inflammation , fibrosis , and epithelial - mesenchymal transition ( EMT ) in the pathological process of HCC . - pubmed
BACKGROUND : The barrier dysfunction in atopic dermatitis ( AD ) skin correlates with stratum corneum ( SC ) lipid abnormalities including reduction of global lipid content , shorter ceramide ( CER ) as well as free fatty acid ( FFA ) chain length and altered CER subclass levels . - pubmed
AIM : We investigated whether the expression of CER and FFA biosynthesis enzymes are altered in AD skin compared with control skin and determine whether changes in enzyme expression can be related with changes in lipid composition . - pubmed
These enzymes include stearoyl CoA desaturase ( SCD ) , elongase 1 ( ELOVL1 ) and ELOVL6 involved in FFA synthesis and beta - glucocerebrosidase ( GBA ) , acid - sphingomyelinase ( aSmase ) , ceramide synthase 3 ( CerS3 ) involved in CER synthesis . - pubmed
This was accompanied by functional changes displayed by increased unsaturated FFAs ( SCD ) and reduced FFA C22 - C28 ( ELOVL1 ) in AD lesional skin . - pubmed
The CER composition in AD lesional skin showed corresponding changes such as increased CER AS and NS ( aSmase ) and decreased esterified omega - hydroxy CERs ( CerS3 ) . - pubmed
METHODS : The MAP consists of mandatory pre - operative etoricoxib , intra - operative acetaminophen , and post - operative acetaminophen with optional post - operative tramadol . - pubmed
Assignment of the chemical shifts of the backbone atoms ( HN , N , CA , CB , and C ) of proteins is often a prerequisite to using NMR information in the study of proteins . - pubmed
Furthermore , molecular docking studies suggested that 4h binds to the colchicine binding site of tubulin . - pubmed
Cyclodipeptide c(Orn - Pro ) Conjugate with 4 - Ethylpiperic Acid Abrogates Cancer Cell Metastasis through Modulating MDM2 . - pubmed
The present work describes the synthesis , characterization , and anticancer properties of c ( Lys - Pro ) , P1 ; c ( Orn - Pro ) , P2 ; and conjugates PA - c ( Lys - Pro ) , C1 ; PA - c ( Orn - Pro ) , C2 ; EPA - c ( Lys - Pro ) , C3 ; and EPA - c ( Orn - Pro ) , C4 . - pubmed
RESULTS : TNF - alpha - induced TSLP expression was inhibited by the EGFR kinase inhibitor AG1478 . - pubmed
While TSLP expression was induced by EGF , it was inhibited by the MEK inhibitor , U0126 . - pubmed
The open reading frames of RcWRI1 - A ( 1341 bp ) and RcWRI1 - B ( 1332 bp ) differ by a stretch of 9 bp , such that the predicted RcWRI1 - B lacks the three amino acid residues " VYL " that are present in RcWRI1 - A. - pubmed
When the two isoforms were individually introduced into an Arabidopsiswri1 - 1 loss - of - function mutant , total fatty acid content was almost restored to the wild - type level , and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the wri1 - 1 mutant line . - pubmed
Both RcWRI1 - A and RcWRI1 - B can be used as a key transcriptional regulator to enhance fatty acid and oil biosynthesis in leafy biomass . - pubmed
Anti - cancer Effects of HNHA and Lenvatinib by the Suppression of EMT - Mediated Drug Resistance in Cancer Stem Cells . - pubmed
This study aimed to develop targeted agents against thyroid malignancy , focusing on individual and synergistic effects of HNHA ( histone deacetylase ) , lenvatinib ( FGFR ) , and sorafenib ( tyrosine kinase ) inhibitors . - pubmed
HNHA - lenvatinib combined treatment induced markers of cell cycle arrest and apoptosis and suppressed anti - apoptosis markers , epithelial - mesenchymal transition ( EMT ) , and the FGFR signaling pathway . - pubmed
HNHA - lenvatinib combination treatment thus blocked the FGFR signaling pathway , which is important for EMT . - pubmed
ANOVA showed that class of film , fungal species , aw and temperature and their interactions significantly affected growth rates ( GR ) , ED50 and ED90 and the doses for total fungal growth inhibition and OTA production . - pubmed
Depending on the species , aw and temperature affected GR and OTA production in a different way . - pubmed
Selenoprotein P ( SeP ) , a liver - derived secretory protein , functions as a selenium supply protein in the body . - pubmed
Cellular assays were carried out for chemotaxis ( agarose spot assay ) , cell motility ( live cell imaging ) , metabolism ( MTT ) and cell polarisation ( CK18 ; ezrin ) . - pubmed
Several analogues of ( + ) - ursolic acid ( 1 ) were synthesized , and a preliminary structure - activity relationship ( SAR ) study indicated that the C-3 hydroxy and C-28 carboxylic acid groups and 19,20 - dimethyl substitution are all essential in the mediation of the bioactivities observed for this triterpenoid . - pubmed
A multivariate logistic regression analysis was used to examine the effects of sociodemographic characteristics , lifestyle , activity status , physical exercise , systolic blood pressure , low - density lipoprotein ( LDL ) , high - density lipoprotein ( HDL ) , triglycerides , body mass index ( BMI ) , and CRP levels on depressive symptoms . - pubmed
After adjusting for age , education , marital status , hukou , residence , cigarette smoking , alcohol drinking , eating meals , activity status , major accidental injury , diseases , health status , physical exercise , systolic blood pressure , LDL , HDL , triglycerides , and BMI , depressive symptoms were not associated with subsequent high CPR levels among the middle - aged and elderly ( CRP 1.01 - 3.00 mg / L : OR = 0.93 , 95 % CI = 0.83 - 1.03 ; CRP 3.01 - 10.00 mg / L : OR = 0.95 , 95 % CI = 0.82 - 1.10 ; CRP & gt;10 mg / l : OR = 1.11 , 95 % CI = 0.88 - 1.39).Our data do not support an association between CRP and depressive symptoms in both middle - aged and elderly men and women among china . - pubmed
The role of TDM in optimizing vedolizumab , ustekinumab , and tofacitinib remains unclear , given differences in pharmacokinetics and immunogenicity compared to TNF antagonists . - pubmed
In addition to the original models for CID and HCD fragmentation , we have added specialized models for the TripleTOF 5600 + mass spectrometer , for TMT - labeled peptides , for iTRAQ - labeled peptides , and for iTRAQ - labeled phosphopeptides . - pubmed
Molecular dynamics ( MD ) revealed that the root mean - square deviation ( RMSD ) , root mean square fluctuation ( RMSF ) , radius of gyration ( Rg ) , and the number of hydrogen and hydrophobic bonds between TQ and residues of BCL-2 , BCL - XL and MCL-1were significantly ( P<0.001 ) changed . - pubmed
Background : The retinol binding protein 4 ( RBP4 ) is essential in delivering retinol to the retinal pigment epithelium and normal functioning of the visual cycle . - pubmed
Acetonyl Peroxy and Hydro Peroxy Self- and Cross- Reactions : Kinetics , Mechanism , and Chaperone Enhancement from the Perspective of the Hydroxyl Radical Product . - pubmed
After treatment with raw cornstarch , the total triglycerides ( TG ) level has significantly decreased by 30 + -50 % ( 8.37 + -7.23 to 5.39 + -5.29 mmol / L , p<0.001 ) . - pubmed
Oxygen saturation was evaluated at baseline ( T0 ) , just after the arterial embolization ( T1 ) , at 10 minutes ( T2 ) , at 20 minutes ( T3 ) , and at 40 minutes ( T4 ) . - pubmed
We quantified brain cell death using serum levels of biomarkers of glial and neuronal cell death ( Glial Fibrillary Acidic Protein ( GFAP ) , Ubiquitin Carboxy - terminal Hydrolase - L1 ( UCH - L1 ) , S100B and Alpha II Spectrin Breakdown Product 150 ( SBDP ) ) in the Biomarkers of Injury and Outcome - Progesterone for Traumatic Brain Injury , Experimental Clinical Treatment ( BIO - ProTECT ) study . - pubmed
Serum progesterone levels were measured at 24 and 48 hours post - injury . - pubmed
Similarly , at 24 and 48 hours post - injury , there were no differences in biomarker levels in the progesterone versus placebo groups ( p>0.15 ) . - pubmed
We conclude that progesterone treatment does not decrease the levels of biomarkers of glial and neuronal cell death during the first 48 hours post - injury . - pubmed
Coupled porosity and heterojunction engineering : MOF - derived porous Co3O4 embedded on TiO2 nanotube arrays for water remediation . - pubmed
METHODS : This cross - sectional , case - control study evaluates associations among OC mucus inflammatory proteins , psychophysical olfactory testing , and computed tomography ( CT ) analysis of the OC and sinuses . - pubmed
At rest , the heart rate was significantly lower on metoprolol than on practolol or H 87/07 . - pubmed
On the contrary the correlation of total and free thyroid hormone concentrations with the response of TSH to TRH was not significant . - pubmed
The amino acid sequence of polypeptide II from beef heart cytochrome c oxidase is described . - pubmed
Thus subunit II of the oxidase is a member of this copper protein family . - pubmed
Thus subunit II is like a blue copper protein with type I copper . - pubmed
In further study of these specimens , an immunoperoxidase staining technique was used to stain for hCG in formalin - fixed sections . - pubmed
Eserine sulphate ( 10(-5 ) M ) was used as inhibitor for AChE. - pubmed
Ganglioside - induced CD4 endocytosis occurs independent of serine phosphorylation and is accompanied by dissociation of P56lck . - pubmed
Gangliosides induce a selective and complete modulation of CD4 from the surface of T cells . - pubmed
This process is independent of serine phosphorylation of the cytoplasmic tail of CD4 and does not require the association between the tyrosine protein kinase p56lck and the cytoplasmic tail of CD4 . - pubmed
Ganglioside - induced CD4 endocytosis is accompanied by the loss of p56lck activity associated with CD4 . - pubmed
The kinetics of p56lck dissociation after ganglioside treatment is identical to that of CD4 endocytosis , suggesting that p56lck is displaced in the process of endosome formation . - pubmed
Gonadotropin - releasing hormone ( GnRH ) agonists show promise as new therapeutic agents with considerable potential for clinical applications in endocrinology , gynecology , pediatrics , and oncology . - pubmed
GnRH agonists are derived from GnRH . - pubmed
In as much as PBN contains mu and kappa but no detectable delta receptors , the present results suggest that mu opioid activity within the PBN is involved in the mediation of feeding behavior . - pubmed
DNA was isolated from the peripheral blood lymphocytes and aromatic DNA adducts were detected by 32P - postlabelling assay using the nuclease P1 digestion procedure for the enrichment of the adducts . - pubmed
Induction of preterm labour by infusion of ACTH ( 66.6 ng / min for 15 min every 2 h ) to the fetus resulted in a reduction in plasma progesterone concentrations and increases in values of oestradiol-17 beta and 13,14 - dihydro 15 - keto PGF-2 alpha in maternal plasma . - pubmed
When cell growth was inhibited with mevinolin , a cholesterol synthesis inhibitor , the addition of LDL to the medium had no effect on the growth of cells from five of six tumor cell lines . - pubmed
Thus , five of six colon tumor cell lines were unable to overcome a mevinolin block in cholesterol metabolism indicating that these cells were deficient in LDL receptors . - pubmed
Plasma atrial natriuretic peptide was increased and was not significantly modified by infusion of hypertonic saline or acute administration of furosemide . - pubmed
In the neutral extracts an immunoreactive somatostatin of unidentified molecular structure dominated quantitatively over somatostatin 14 and 28 , which were the major components in acetic acid extracts . - pubmed
The recent availability of monoclonal antibodies immunoreactive to T - or B - lineage antigens in formalin - fixed , paraffin - embedded tissue has permitted the adaptation of frozen - section immunodiagnostic criteria to paraffin - embedded tissue . - pubmed
Arginine vasopressin increases the IR - ACTH response to oCRF fourfold when given simultaneously with oCRF . - pubmed
Salivary lysozyme , peroxidase , and hypothiocyanite concentrations were already at the adult level at the time when the primary teeth erupt , whereas immunoglobulin ( IgA , IgG , and IgM ) , lactoferrin , myeloperoxidase , and thiocyanate concentrations were significantly lower in children than in adults . - pubmed
Dentate children had more IgG , thiocyanate , and protein in whole saliva than did predentate children . - pubmed
In both anemic and nonanemic patients serum ferritin levels decreased and total iron - binding capacity increased as gestation progressed . - pubmed
The total iron - binding capacity is higher in maternal than in umbilical cord blood at delivery ; this suggests that little or no ferritin or transferrin is transferred from mother to fetus . - pubmed
The removal of transferrin from the surface of the trophoblast by thiocyanate and its rebinding were demonstrated . - pubmed
Creatine and the control of myosin synthesis in differentiating skeletal muscle . - pubmed
Skeletal muscle cells formed both in vitro and in vivo synthesize myosin heavy chain faster when supplied creatine in vitro . - pubmed
Gamma - glutamyltransferase activity was present in very few of the spontaneous neoplasms or in the neoplasms induced in the absence of phenobarbitone administration . - pubmed
GA and HS selectively inhibited the DMPP - induced contraction without having an antagonistic effect on the McN - A-343 - induced and acetylcholine - induced contraction . - pubmed
In addition , since GA and HS showed a local anesthetic action weaker than that of procaine , the effect of procaine was studied on the same preparation . - pubmed
This same component accumulates label at an accelerated rate in the presence of the protease inhibitor leupeptin . - pubmed
The proarrhythmic effects of the ATP - sensitive potassium channel modulators cromakalim ( n = 10 ; 0.01 to 0.3 mg / kg i.v . ) - pubmed
; n = 7 ) , or Vasopressin ( VP : 0.8 U / kg B.W. - pubmed
The response of forearm blood flow to sodium nitroprusside was greater when subjects received a high sodium diet than when they received a low sodium diet ( F = 7.11 , P & lt ; .05 ) ; however , the response to methacholine was not altered by sodium intake ( F = 0.57 , P = NS ) . - pubmed
However , increasing resistance to ceftazidime and other third generation cephalosporins , particularly among Enterobacteriaceae , due to the emergence of plasmid - mediated extended spectrum beta - lactamases and the class I chromosomally mediated beta - lactamases , is of concern . - pubmed
We have used intravenous gammaglobulin ( IVIG ; 10 % Gamimune N ) to determine both its efficacy in reducing panel - reactive antibodies in vitro and the prognostic value of the in vitro testing for in vivo efficacy . - pubmed
Biochemical studies to determine the effective component of IVIG show that it is the IgG fraction and not soluble antigen or the minor IgM or IgA contaminants that is responsible . - pubmed
An increase in [ Ca2+]i in neurons B5 was also induced by a PTH agonist [ bPTH -( 1 - 34 ) ] , but not by a PTH antagonist [ bPTH -( 3 - 34 ) ] . - pubmed
Furthermore , our results demonstrated that the effect of PTH on [ Ca2 +]i in neurons B5 was not due to the hormonal modulation of voltage - dependent Na + or K + channels or a Na +/ Ca2 + exchanger . - pubmed
Serum cortisol concentration was measured before and after adrenocortical stimulation with 100 IU of adrenocorticotropic hormone , 1 week prior to TA administration , and 4 and 8 weeks thereafter . - pubmed
Both compounds resulted in decreased binding of conA and WGA to cell surface oligosaccharides showing they were causing a reduction in N - linked cell surface glycoproteins . - pubmed
Changes in the plasma concentration of adrenaline were produced by varying the rate of an intravenous infusion of adrenaline and in the effects of 5 - HT , by intravenous injections of the selective 5 - HT2 receptor antagonist , ICI 170809 . - pubmed
Antagonism of the 5 - HT2 receptor by ICI 170809 , in the absence of an infusion of adrenaline , abolished thrombus formation ( mean ED50 0.41 microgram kg-1 , i.v . ) - pubmed
The locations of [ 3H ] thymidine - labeled neurons were analysed by autoradiography eight to 16.5 months post - grafting . - pubmed
N - Ethylmaleimide ( NEM ) , an inhibitor of H (+)- ATPase , reduced the cellular pH ( pHi ) dose - dependently . - pubmed
1 x 10(-7 ) M AVP also increased [ Ca2+]i from 111.2 to 189.3 nM , which was significantly reduced by NEM in a dose - dependent manner . - pubmed
These results indicate that H (+)- ATPase participates the cellular action of AVP mediated via the pHi control in renal papillary collecting tubule cells . - pubmed
Liver and kidney GST and liver GR activities were significantly increased after AAF administration . - pubmed
Kidney GPx activities were significantly affected ; activity assayed with cumene hydroperoxide ( cu - OOH ) was increased but activity assayed with H2O2 was reduced . - pubmed
Design and synthesis of C - linked fucosides as inhibitors of E - selectin . - pubmed
Two series of C - linked fucosides as mimetics for the tetrasaccharide sialyl Lewis X have been synthesized and tested as inhibitors of E - Selectin . - pubmed
In addition , BP170 was co - expressed with tyrosine hydroxylase which suggests its possible role in the dopamine pathway . - pubmed
Differential effects of gonadotropin - releasing hormone ( GnRH ) pulse frequency on gonadotropin subunit and GnRH receptor messenger ribonucleic acid levels in vitro . - pubmed
The hypothalamic hormone , GnRH , is released and transported to the anterior pituitary in a pulsatile manner , where it binds to specific high - affinity receptors and regulates gonadotropin biosynthesis and secretion . - pubmed
The frequency of GnRH pulses changes under various physiological conditions , and varying GnRH pulse frequencies have been shown to regulate differentially the secretion of LH and FSH and the expression of the gonadotropin alpha , LH beta , and FSH beta subunit genes in vivo . - pubmed
Cells were treated with 10 nM GnRH pulses for 24 h at a frequency of every 0.5 , 1 , 2 , or 4 h. alpha , LH beta , and FSH beta messenger RNA ( mRNA ) levels were increased by GnRH at all pulse frequencies alpha and LH beta mRNA levels and LH secretion were stimulated to the greatest extent at a GnRH pulse frequency of every 30 min , whereas FSH beta mRNA levels and FSH secretion were stimulated maximally at a lower GnRH pulse frequency , every 2 h. GnRH receptor ( GnRHR ) mRNA levels also were increased by GnRH at all pulse frequencies and were stimulated maximally at a GnRH pulse frequency of every 30 min . - pubmed
This suggests that alpha and LH beta mRNA levels are maximally stimulated when GnRHR levels are relatively high , whereas FSH beta mRNA levels are maximally stimulated at lower levels of GnRHR expression , and that the mechanism for differential regulation of the gonadotropins by varying pulse frequencies of GnRH may involve levels of GnRHR . - pubmed
Furthermore , these data suggest that the mechanisms whereby varying GnRH pulse frequencies stimulate alpha LH beta , and GnRHR gene expression are similar , whereas the stimulation of FSH beta mRNA levels may be different . - pubmed
Most parasympathetic axons contained IR for vasoactive intestinal peptide ( VIP ) and neuropeptide Y ( NPY ) , with or without enkephalin ( Enk ) . - pubmed
PURPOSE : Our experience with IVF using low - dose clomiphene citrate for stimulation in " non- " and " poor " responders was reviewed and the treatment outcomes with the previous controlled ovarian stimulation cycles in which hMG and GnRH agonist were used were compared . - pubmed
METHODS : The treatment outcome in 11 non- and 20 poor responders having 30 and 53 clomiphene citrate IVF treatment cycles , respectively , were compared with the treatment outcome in the previous long - protocol buserelin / hMG cycles . - pubmed
RESULTS : The clinical pregnancy rates per oocyte collection achieved in the first clomiphene citrate cycle in non ( 9.1%)- and poor ( 10 % ) responders were comparable to those achieved by poor responders ( 11.9 % ) who had buserelin / hMG using the long protocol . - pubmed
CONCLUSIONS : IVF using long - protocol buserelin / hMG is more successful than using clomiphene citrate stimulation . - pubmed
However , this advantage may not be significant in those women with a previous poor response to buserelin / hMG . - pubmed
It is suggested that for such poor responders , three attempts of IVF in a clomiphene citrate cycle may offer a viable therapeutic alternative before reverting to more stressful , expensive , and time - consuming treatment . - pubmed
Flavopiridol is known to inhibit potently the activity of multiple cyclin - dependent kinases . - pubmed
Flavopiridol , when administered as daily bolus intravenous ( IV ) injections , produced selective apoptosis of cells in the thymus , spleen , and lymph nodes , resulting in atrophy of these organs . - pubmed
After treatment with 7.5 mg / kg flavopiridol bolus IV or intraperitoneal on each of 5 consecutive days , 11 out of 12 advanced stage subcutaneous ( s.c . ) - pubmed
We discovered that compounds with 26 membered rings of [ Cys4,D - Nal7,Cys11 ] alpha - MSH(4 - 11 ) displayed specific MC4 receptor selectivity . - pubmed
The preference order of the different MC receptor subtypes for the novel [ Cys4D - Nal7Cys11 ] alpha - MSH(4 - 11 ) analogues are distinct from all other known MSH analogues , particularly as they bind the MC4 receptor with high and the MC1 receptor with low relative affinities . - pubmed
HS964 and HS014 have 12 and 17 fold MC4 / MC3 receptor selectivity , respectively , which is much higher than for the previously described cyclic lactam and [ Cys4,Cys10 ] alpha - MSH analogues SHU9119 and HS9510 . - pubmed
HS964 is the first substance showing higher affinity for the MC5 receptor than the MC1 receptor . - pubmed
We found that a compound with a 29 membered ring of [ Cys3,Nle10,D - Nal7,Cys11 ] alpha - MSH(3 - 11 ) ( HS010 ) had the highest affinity for the MC3 receptor . - pubmed
In order to improve both quantitative and qualitative thalassemia erythropoiesis , several trials were undertaken combining rHuEPO with hydroxyurea ( HU ) , which is known to increase % Hb F , MCV and MCH without a major effect on Hb levels . - pubmed
The designed trial included 3 to 6 months of HU alone ( 20 mg / kg x 4 / week ) , or with rHuEPO alone ( 500 u / kg x 3 / week or 375 u / kg x 2 / week ) or a combination of the two drugs . - pubmed
It is not known whether the addition of oral iron to rHuEPO is warranted for maximal erythropoietic response . - pubmed
Calcium - binding proteins , such as S-100 , dimerize readily , and this phenomenon plays an important role in their regulation of target enzymes [ Krebs , J. , Quadroni , M. & amp ; Van Eldik , L.J. - pubmed
We have investigated by Fourier - transform ion cyclotron resonance ( FTICR ) MS the conformational states of the calcium - binding protein calmodulin , and present clear evidence for a calmodulin dimer formed as a result of noncovalent interactions between folded monomers . - pubmed
With the use of denaturing solutions ( 1 : 1 acetonitrile / water + 1 % formic acid ) , relatively high charge states ( 20 & lt ; z & lt ; 10 ) of monomeric calmodulin ions were detected , whereas when calmodulin was electrosprayed from buffer , monomers ions with only 5 - 10 charges were detected . - pubmed
CD measurements for calmodulin in buffered solution revealed that its alpha - helical content was significantly higher than that for calmodulin in acetonitrile / water solutions , consistent with a proposition that changes in charge state distributions observed in the MS experiments reflect differing states of calmodulin folding . - pubmed
Superoxide dismutase activity and reduced glutathione content were not significantly different in either cell type when challenged with Ox or Ox + COM . - pubmed
Effect of lovastatin on small GTP binding proteins and on TGF - beta1 and fibronectin expression . - pubmed
It was postulated that this down - regulation may be linked to signaling of small guanine triphosphate ( GTP )- binding proteins and mediated by the limitation of isoprenoids such as farnesylpyrophosphate ( FPP ) and geranylgeranylpyrophosphate ( GGPP ) in RMC . - pubmed
To determine the isoprenoid and small GTP - binding proteins involved in TGF - beta1 and fibronectin expression . - pubmed
Suppression of TGF - beta1 and fibronectin expression by lovastatin was reversed effectively when GGPP was added alone . - pubmed
Partial reversal of lovastatin effect on fibronectin and TGF - beta1 expression was found when FPP was added alone . - pubmed
Adding both GGPP and FPP resulted in complete reversal of lovastatin effect on fibronectin but not TGF - beta1 suggesting that fibronectin and TGF - beta1 are regulated differently . - pubmed
Intracellular reduction of selenite into glutathione peroxidase . - pubmed
Selenium ( Se ) in selenite is present in an oxidized state , and must be reduced for it to be incorporated as selenocysteine into selenoenzymes such as glutathione peroxidase ( GPx ) . - pubmed
In vitro , Se , as in selenite , can be reduced utilizing glutathione ( GSH ) and glutathione reductase ( GRed ) . - pubmed
Buthionine sulfoximine ( BSO ) was used to decrease GSH levels , 1,3 bis -( 2 chloroethyl )- 1 - nitrosourea ( BCNU ) was used to inhibit GRed activity and methylene blue ( MB ) was used to decrease cellular NADPH levels . - pubmed
This statistical method evaluates the effects of BSO , BCNU , MB and selenite alone and in combinations on GPx activity . - pubmed
For cells with abnormal ability to generate NADPH , lowering the GSH levels had a small effect on selenite - dependent GPx synthesis . - pubmed
GRed activity is not required for the selenite - dependent synthesis of GPx . - pubmed
All of the dppA - disrupted mutants produced a normal level of native form of DMSOR and grew by DMSO respiration , indicating that the loss of DppA protein alone had no effect on the formation of active DMSOR . - pubmed
Cocaine and amphetamine regulated transcript ( CART ) peptide has been shown to be an anorectic peptide that inhibits both normal and starvation - induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus . - pubmed
The C - terminal part containing the three disulfide bridges CART(48 - 89 ) is the biologically active part of the molecule affecting food intake . - pubmed
CART consists mainly of turns and loops spanned by a compact framework composed by a few small stretches of antiparallel beta - sheet common to cystine knots . - pubmed
1.4.3.6 ) , the enzymes which control membrane properties ( LOX ) and the catabolism of polyamines ( DAO ) , respectively . - pubmed
Although irbesartan had no effect on glucose transport ( 2 - deoxyglucose uptake ) in the epitrochlearis muscle , which consists mainly of type IIb fibers , acute angiotensin II receptor antagonism led to a dose - dependent increase in insulin action in the predominantly type I soleus muscle . - pubmed
Irbesartan at 25 and 50 mg / kg induced significant increases ( 41 % and 50 % , respectively ; P<0.05 ) in insulin - mediated glucose transport . - pubmed
Moreover , these acute irbesartan - induced improvements in soleus - muscle glucose transport were associated with enhancements in whole - body insulin sensitivity ( r=-0.732 ; P<0.05 ) , as assessed during an oral glucose tolerance test . - pubmed
d(-1 ) ) , glucose tolerance was enhanced further , and insulin - mediated glucose transport was significantly elevated in both epitrochlearis ( 32 % ) and soleus ( 73 % ) muscle . - pubmed
Chronic irbesartan - induced increases in whole - body insulin sensitivity were associated with increased insulin - mediated glucose transport in both epitrochlearis ( r=-0.677 ; P<0.05 ) and soleus ( r=-0.892 ; P<0.05 ) muscle . - pubmed
In summary , angiotensin II receptor ( AT ( 1 )- subtype ) antagonism , either acutely or chronically , improves glucose tolerance , at least in part because of an enhancement in skeletal - muscle glucose transport , and the effect of chronic angiotensin II receptor antagonism on type I skeletal - muscle glucose uptake is associated with an increase in GLUT-4 protein expression . - pubmed
These results show that residues around the NPA1 box can influence the folding of the MIP in the membrane , and provide structural evidence for the poor water transport properties of MIP , as the NPA boxes lie outside the plane of the membrane . - pubmed
BACKGROUND : Xanthurenic acid is an endogenous product of tryptophan degradation by indoleamine 2,3 - dioxygenase ( IDO ) . - pubmed
In the control cells gelsolin stained the perinuclear region , whereas in the presence of 10 microM xanthurenic acid gelsolin is translocated to the cytoskeleton , but does not lead to cytoskeleton breakdown . - pubmed
At low ( 5 to 10 microM ) of xanthurenic acid concentration , the elongation of the cytoskeleton was associated with migration of mitochondria and cytochrome c release . - pubmed
We observed an induction of calpain Lp 82 and an increase of free Ca2 + in the cells in a xanthurenic acid concentration - dependent manner . - pubmed
Xanthurenic acid induces calpain Lp82 and caspases in the cells growing in the dark and can be involved in senile cataract development . - pubmed
PURPOSE : To review the uses and previously proposed mechanisms of action of amniotic membrane transplantation ( AMT ) and to suggest a new mechanism of action for the effectiveness of AMT in resolving a persistent epithelial defect ( PED ) . - pubmed
Electron microscopic determination of silver incorporation in collagen fibers as a model of organic - metal chiral supramolecular structure with optical anisotropic properties . - pubmed
The high positive linear dichroism ( LD ) exhibited by silver - impregnated collagen fibers are thought to be caused by an ordered binding of silver nanoparticles to the collagen fibers . - pubmed
A correlation between LD , established by image analysis , and the size of silver particles as measured by electron microscopy was investigated in the same sections of silver - impregnated tendon collagen fibers . - pubmed
Silver particles were found to be periodically ordered along the collagen fibers , with some preference for the 67 nm collagen period and collagen a bands . - pubmed
The finding that interference colors are correlated to abnormal dispersion of birefringence characteristics supports the concept that collagen fibers induce an ordered binding of silver distributed along the fibers . - pubmed
Hepatocyte prostaglandin synthesis and Mdr1 expression . - pubmed
The most common ART was zidovudine / lamivudine ( 39 % ) followed by zidovudine - lamivudine - nelfinavir ( 34 % ) , although 34 combinations were used . - pubmed
TRP , 5 - hydroxyindoleacetic acid ( 5 - HIAA ) , kynurenine ( KYN ) , and interleukin-6 ( IL-6 ) were measured . - pubmed
A chronic colitis was induced by oral administration of 5 % dextran sulfate sodium ( DSS ) for 9 days followed by 2 % DSS for 18 days . - pubmed
Catechol - O - methyltransferase ( COMT ) is an enzyme that inactivates catecholamines , including levodopa . - pubmed
An amino acid change ( Val-108 - Met ) in the COMT protein has been found to result in a change from high to low enzyme activity . - pubmed
Dynamic aspect of bacteriorhodopsin as a typical membrane protein as revealed by site - directed solid - state 13C NMR . - pubmed
We demonstrate here a general feature of dynamic aspect of membrane proteins as revealed by site - directed 13C NMR studies on bacteriorhodopsin ( bR ) as a typical membrane protein and a variety of mutants at ambient temperature . - pubmed
It is emphasized that the observed displacement of [ 3 - 13C]-labeled Ala 184 peak at 17.4 ppm in the presence of kinked structure in this model peptide is consistent with that of intact protein at 17.27 ppm . - pubmed
Maintenance immunosuppression regimens after the first and second grafts were similar , consisting of a combination of a calcineurin inhibitor , mycophenolate mofetil , and prednisone . - pubmed
We have previously shown that hormone therapy ( HT ) with medroxyprogesterone acetate ( MPA ) alone or in combination with conjugated equine estrogens ( CEE ) impairs insulin sensitivity . - pubmed
In the current study , we sought to determine if the effect of MPA on whole body insulin sensitivity is associated with alterations in insulin signaling proteins in skeletal muscle . - pubmed
There was a tendency for decreased insulin receptor tyrosine phosphorylation with CEE + MPA treatment ( ANOVA P = 0.14 ) . - pubmed
The cardiac slow delayed rectifier potassium channel ( IKs ) , comprised of ( KCNQ1 ) and beta ( KCNE1 ) subunits , is regulated by sympathetic nervous stimulation , with activation of beta - adrenergic receptors PKA phosphorylating IKs channels . - pubmed
In IKs / B2 - AR myocytes , IKs density was increased , and activation shifted in the hyperpolarizing direction ; IKs was not further modulated by exposure to isoproterenol , and KCNQ1 was found to be PKA - phosphorylated . - pubmed
Conversely , beta2 - AR overexpression did not affect L - type calcium channel current ( ICaL ) under basal conditions with ICaL remaining responsive to cAMP . - pubmed
The G - allele carriers of the SREBF-1 gene C - G polymorphism in exon 18c and coding for glycine at the protein level ( G952 G ) have shown to associate more frequently with obesity and type 2 diabetes than the C - allele carriers . - pubmed
Similarly muscle cholesterol ( p=0.045 ) and lathosterol ( p=0.054 ) concentrations were elevated in the CC homozygotes supporting the view that endogenous cholesterol synthesis rate is SREBF-1 genotype - dependent . - pubmed
Metabolism of levodopa via the enzyme catechol - O - methyltransferase requires S - adenosylmethionine ( SAM ) as a methyl donor . - pubmed
Plasma levels of SAM and levodopa significantly increased , but MET concentrations did not significantly decrease . - pubmed
RESULTS : The mean total cholesterol ( TC ) , low - density lipoprotein cholesterol ( LDLC ) , and triglyceride ( TG ) levels were similar in men and women , while the mean HDLC level was significantly higher in women than men ( 58.3+/-18.1 vs. 50.3+/-13.5 mg / dL ; p=0.0064 ) . - pubmed
piscifermentans ; lactic acid production , urease activity , bacteriocin production and the antibiotic susceptibility of it were also determined . - pubmed
Kinetics of hydrolysis of acetylcholine and acetylthiocholine by two types of acetylcholinesterase and butyrylcholinesterase inhibited by 13 new inhibitors ( 5 carbamates and 8 carbazates -- hydrazinium derivatives ) was measured in vitro in a batch reactor at 25 degrees C , pH 8 , ionic strength 0.11 M and enzyme activity 3.5 U by four nondependent analytical methods . - pubmed
Kinetics of hydrolyses inhibited by all studied carbamates , sevin , carbazates ( with exceptions ) and rivastigmin ( with exceptions ) can be simulated by the competitive inhibition model with irreversible reaction between enzyme and inhibitor . - pubmed
The tested carbazates failed as inhibitors of cholinesterases . - pubmed
Although the mechanisms are not understood , evidence suggests that 17beta - estradiol ( E2 ) confers protection from cardiovascular and renal complications in many diseases . - pubmed
There was no change in ERalpha or AT1R binding following Cas + /- DHT ( 25 mg ) treatment , although Cas did elevate cortical ERbeta ( P & lt ; 0.01 ) . - pubmed
Interestingly , the high dose DHT ( 200 mg ) elevated ERalpha 150 % above intact levels and profoundly decreased AT1R binding ( 1,824 + /- 705 cpm , P & lt ; 0.001 vs. intact male ) . - pubmed
Furthermore , E2 regulates ERalpha and is inversely associated with glomerular AT1R binding , supporting our hypothesis that E2 tonically suppresses AT1Rs and suggesting a potential mechanism for the protective effects of estrogen . - pubmed
Correlations between the level of fructosamine and other variables including the results of glucose challenge test ( GCT ) , oral glucose tolerance test ( OGTT ) , and glycosylated hemoglobin ( HbA1c ) test , and infant 's birth weight were analyzed by Pearson correlation . - pubmed
Molecular insights into nitrogenase FeMoco insertion -- the role of His 274 and His 451 of MoFe protein alpha subunit . - pubmed
The final step of FeMo cofactor ( FeMoco ) assembly involves the insertion of FeMoco into its binding site in the molybdenum - iron ( MoFe ) protein of nitrogenase . - pubmed
Our results from combined metal , activity , EPR , stability and insertion analyses show that mutations of His alpha274 and/or His alpha451 , two of the histidines that belong to a so - called His triad , to small uncharged Ala specifically reduce the accumulation of FeMoco in MoFe protein . - pubmed
Subunit H of the vacuolar ( H + ) ATPase inhibits ATP hydrolysis by the free V1 domain by interaction with the rotary subunit F. The vacuolar ( H + ) ATPases ( V - ATPases ) are large , multimeric proton pumps that , like the related family of F1F0 ATP synthases , employ a rotary mechanism . - pubmed
ATP hydrolysis by the peripheral V1 domain drives rotation of a rotary complex ( the rotor ) relative to the stationary part of the enzyme ( the stator ) , leading to proton translocation through the integral V0 domain . - pubmed
Unlike the corresponding domains in F1F0 , the dissociated V1 domain does not hydrolyze ATP , and the free V0 domain does not passively conduct protons . - pubmed
275 , 21761 - 21767 ) showed that subunit H ( part of the stator ) inhibits ATP hydrolysis by free V1 . - pubmed
To test the hypothesis that subunit H accomplishes this by bridging rotor and stator in free V1 , cysteine - mediated cross - linking studies were performed . - pubmed
Our experiments suggest that activation Insig-1 by drugs leads to reduced levels of active Srebp-1 and consequently to reduced target gene expression including the genes responsible for triglyceride synthesis . - pubmed
In addition , observed that Insig-1 is also a target of AMP - activated kinase , the hepatic activity of which is increased by activators of CAR and PXR and is known to cause a reduction of triglycerides . - pubmed
The analyte was isocratically eluted on a Venusil XBP C8 column ( 200 mm x 3.9 mm ID , 5 microm ) with methanol - water mol x L(-1 ) ammonium formate ) ( 80:20 ) at a flow rate of 0.4 mL x min(-1 ) , and detected ( containing 5 mmol x L(-1 ) ammonium formate ) ( 80:20 ) at a flow rate of 0.4 mL x min(-1 ) , and detected with a triple quad LC - MS / MS using ESI with positive ionization . - pubmed
Among the discordant cases , MRI was superior in demonstrating tumor involvement in the parapharyngeal space , skull base , intracranial area , sphenoid sinus , and retropharyngeal nodes while PET / CT was superior in demonstrating neck nodal metastasis . - pubmed
Folate binds to dihydrofolate reductase ( DHFR ) to form a binary complex whose structure maintains the overall configuration of the enzyme ; however , some significant changes are evident when a comparison is made to the enzyme . - pubmed
Differential estradiol and selective estrogen receptor modulator ( SERM ) regulation of Keratin 13 gene expression and its underlying mechanism in breast cancer cells . - pubmed
Expression of the Keratin 13 ( KRT13 ) gene , which encodes a cytoskeletal protein thought to play important roles in breast cancer growth and metastasis , is differentially regulated by estradiol ( E2 ) and the selective estrogen receptor modulators ( SERMs ) tamoxifen and raloxifene . - pubmed
While stimulation of KRT13 by tamoxifen is robust and prolonged , stimulation by E2 is more transient and raloxifene has virtually no effect . - pubmed
Directed composite mutations in this region revealed that three estrogen response elements and three Sp1 sites were involved in its ligand - dependent regulation . - pubmed
Differential recruitment of ERalpha and cofactors to the KRT13 regulatory sites paralleled the different time course and magnitude of regulation by these ligands : there was almost no ERalpha or cofactor recruitment with raloxifene , whereas there was strong , prolonged ER recruitment and histone acetylation with tamoxifen , and an early and more transient recruitment with E2 . - pubmed
Taken together , our results suggest that the different ligand regulations of KRT13 are due to ligand - differential recruitment of ER and coactivators , and they provide insight into the mechanisms responsible for the different agonistic activities and differential gene regulation by estradiol and the SERMs tamoxifen and raloxifene . - pubmed
The CPM loaded in the resinates ( drug - loaded resins ) increased with the increasing degree of sulfonic group and hence the drug binding site in the employed resins . - pubmed
The CPM release was lower from the resins with the higher degree of sulfonic group due to the increase in the diffusive path depth . - pubmed
The CPM release was obviously lower in SGF than SIF because CPM , a weak base drug , ionized to a greater extent in SGF and then preferred binding with rather than releasing from the resins . - pubmed
The open reading frame ( ORF ) of 297 nucleotides ( nt ) of Lycstefin encodes a protein of 99 amino acids ( aa ) with a putative molecular weight of 11kDa , in which no signal peptide and potential N - glycoslation site are predicted . - pubmed
Recombinant Lycstefin protein fused with glutathione S - transferase ( rLycstefin ) was shown to have strong inhibitory activity against papain with a K(i ) of 1.3x10(-13)M. The in vivo experiments revealed that Lycstefin could not modulate the expression levels of large yellow croaker tumor necrosis factor - alpha2 ( TNF - alpha2 ) and interleukin-10 in spleen and head kidney . - pubmed
CdS magic - sized nanocrystals exhibiting bright band gap photoemission via thermodynamically driven formation . - pubmed
CdS magic - sized nanocrystals ( MSNs ) exhibiting both band gap absorption and emission at 378 nm with a narrow bandwidth of approximately 9 nm and quantum yield ( QY ) of approximately 10 % ( total QY approximately 28 % , in hexane ) were synthesized via a one - pot noninjection approach . - pubmed
This CdS MSN ensemble is termed as Family 378 . - pubmed
( 13 ) C solid - state cross - polarization magic - angle spinning ( CP / MAS ) nuclear magnetic resonance ( NMR ) demonstrates that the capping ligands are firmly attached to the nanocrystal surface via carboxylate groups . - pubmed
Observation of terahertz vibrations in the nitrogenase FeMo cofactor by femtosecond pump - probe spectroscopy . - pubmed
In addition , CD14(high ) bone marrow monocytes showed relatively high levels of uptake of fluorescently labeled risedronate , which correlated with selective accumulation of unprenylated Rap1A in CD14(+ ) cells , as well as osteoclasts , following treatment with risedronate in vivo . - pubmed
The degradation was found to be enzymatic and dependent on a cofactor , UDP - glucose , suggesting that UDP - glucosyltransferase or other UDP - glucose - dependent enzymes were involved . - pubmed
AIM : To estimate the frequency of relapses of thrombotic and hemorrhagic complications during moderately intensive therapy for antiphospholipid syndrome ( APS ) with warfarin with and without aspirin . - pubmed
, at 1 , 12 and 24h ) , ( 2 ) effects of single ( 1h ) or multiple ( 1 , 12 and 24h ) ethyl pyruvate treatments ( EP ; at 20 or 40 mg / kg , i.p . ) - pubmed
Pyruvate treatment(s ) attenuated CCI - induced reductions of cerebral cytochrome oxidase activity at 7 2h , significantly improving activity in peri - contusional cortex after multiple SP or EP treatments . - pubmed
Ipsilateral CA3(b ) neuronal loss , but not cortical tissue loss , was significantly reduced 1 month post - CCI with pyruvate treatments begun 1h post - CCI . - pubmed
While water and electrolytes can freely pass through the glycocalyx , plasma proteins , especially albumin , interact strongly . - pubmed
RESULTS : The radiochemical purity ( RCP ) of the ( 99m ) Tc - BnAO - NI preparation was greater than 96 % and stable at ambient temperature for 24h ( RCP>90 % ) . - pubmed
The tumor - to - muscle ratios at 2h post - injection of ( 99m ) Tc - BnAO - NI with and without pentoxifylline pretreatment were 1.67 + - 0.38 and 2.59 + - 0.25 , respectively ( p = 0.025 , n = 3 ) . - pubmed
We hypothesized that application of the TLR4 antagonist eritoran may also reduce cardiac hypertrophy after TAC surgery . - pubmed
In contrast , IL-10 was significantly increased in both eritoran groups independent from TAC . - pubmed
CONCLUSION : Application of the TLR4 antagonist eritoran attenuates the development of cardiac hypertrophy possibly by a reduction in inflammatory and increase in anti - inflammatory cytokines . - pubmed
There are some intriguing results and hypotheses about the link between isoprenoid metabolism and the IL-1 pathway through geranylgeranylation that deserve to be further examined . - pubmed
Treatment with the HDAC inhibitor trichostatin A increased the incidence of an abnormal metaphase but had no adverse effect on maturation efficiency . - pubmed
The resulting materials were thoroughly characterized by CHS elemental analysis , X - ray diffraction , transmission electron microscopy , N2 physisorption and H2 chemisorption , as well as by Raman , NMR , FTIR and EPR spectroscopies , in order to investigate the influence of the temperature and atmosphere of the pretreatment on the gold particle size and oxidation state , the formation of defective E ' centres on the silica support and their ability to induce the formation of reactive O2 (- ) surface species . - pubmed
Epitaxial surface growth of oligophenylenes on a heated muscovite mica crystal results in a thin film of mutually aligned nanofibres with dimensions of tens of nanometres in height , hundreds of nanometres in width and tens to hundreds of micrometres in length . - pubmed
Here we used a new MS screening approach to reveal that Ybt is also a physiologically favorable Cu ( II ) ligand . - pubmed
Chemical and genetic characterization showed that Ybt helps bacteria resist copper toxicity by sequestering host - derived Cu ( II ) and preventing its catechol - mediated reduction to Cu ( I ) . - pubmed
Together , these studies reveal a new virulence - associated function for Ybt that is distinct from iron binding . - pubmed
This study presents a systematic detailed experimental and theoretical investigation of the electronic properties of size - controlled free and gamma - Al ( 2 ) O ( 3 )-supported Pt nanoparticles ( NPs ) and their evolution with decreasing NP size and adsorbate ( H ( 2 ) ) coverage . - pubmed
A correlation between the NP size , number of surface atoms and coordination of such atoms , and the maximum hydrogen coverage stabilized at a given temperature is established , with H / Pt ratios exceeding the 1 : 1 ratio previously reported for bulk Pt surfaces . - pubmed
In particular , JVH1 is able to selectively cleave carbon - sulfur bonds within alkyl chains . - pubmed
The variability of peak current of L - type calcium channel ( I(Ca , L ) ) shows an increase in cardiomyocytes after 6 h of preservation when the acutely isolated cardiomyocytes are preserved in a small volume buffer solution . - pubmed
In order to get stable peak I(Ca , L ) , we need to not only select a normal shape of cardiomyocyte at a bright field but also a blue fluorescent myocyte at an ultraviolet excitation . - pubmed
Presence of catalase or indomethacin or SQ 29,548 reduced significantly the pressure - induced myogenic response . - pubmed
Here we verify whether these changes are associated with the occurrence of acute phase response ( APR ) to intravenous ( IV ) zoledronic acid ( ZOL ) or changes of other circulating white blood cells ( WBC ) . - pubmed
Influence of mild and moderate liver impairment on the pharmacokinetics and metabolism of almorexant , a dual orexin receptor antagonist . - pubmed
This single - dose study aimed at investigating the effect of different degrees of hepatic impairment on the pharmacokinetics ( PK ) , metabolism , and tolerability of almorexant , a first - in - class dual orexin receptor antagonist . - pubmed
Twenty - seven VvAP genes were expressed in at least one of the six tissues examined ; nineteen VvAPs responded to at least one abiotic stress , 12 VvAPs responded to powdery mildew infection , and most of the VvAPs responded to SA and ABA treatments . - pubmed
The crystal structure of Rv3717 in complex with a dipeptide product shows that , compared with previously characterized peptidoglycan amidases , the enzyme contains an extra disulfide - bonded beta - hairpin adjacent to the active site . - pubmed
DESIGN : Fibroblasts derived from keloid tissue were incorporated in either Matrigel or polyethylene glycol diacrylate mixed with type I collagen to create 3 - dimensional models to investigate the role MMPs play in keloid formation . - pubmed
The dying cells exhibit FITC - annexin V staining , indicative of programmed cell death ( apoptosis ) . - pubmed
In all 4 , ACTH - stimulated aldosterone concentrations were below the detection limit of the assay . - pubmed
Aldosterone concentrations were not different at 30 , 45 , or 60 minutes after ACTH administration . - pubmed
In this study , an electrochemical method , the cyclic voltammetry method , basing on loop - mediated isothermal amplification ( LAMP ) , electrochemical ion bonding ( positive dye , methylene blue ) , was introduced . - pubmed
The serum adiponectin levels were higher in the healthy group and showed a positive correlation with HDL cholesterol levels in the unhealthy group . - pubmed
According to the number of cysteines in their active site , Grx are classified as dithiolic or monothiolic ( 1 - C - Grx ) . - pubmed
Inositol 1,4,5 - trisphosphate ( IP3 ) is a universal signalling molecule that releases calcium from stores within cells by activating the IP3 receptor . - pubmed
A novel sample preparation method to probe the solution phase structure of dimerized Gramicidin A ( GA ) inserted into lipid vesicle bilayers is described . - pubmed
Solution - phase physicochemical interactions of GA influenced by lipid acyl chain length and extent of acyl chain unsaturation can now be probed by monitoring the conformer preferences using IM - MS . - pubmed
Increasing the acyl chain length from 12 to 22 carbons yields [ 2GA + 2Na ]( 2 + ) IM - MS profiles with reduced conformer microheterogeneity . - pubmed
Superoxide dismutase activity and malondialdehyde content responded efficiently to in situ contamination when a certain degree of contamination was reached . - pubmed
We developed and studied hybrid bilayer wound dressings which combine a drug - loaded porous poly ( dl - lactic - co - glycolic acid ) top layer with a spongy collagen sublayer . - pubmed
Superoxide dismutase ( SOD ) and catalase ( CAT ) activities , along with malondialdehyde ( MDA ) level were determined in skin tissue homogenates of the dermal wounds . - pubmed
macrocarpa fruit extract increased the SOD and CAT activities in the healing wounds , thereby significantly increasing MDA level . - pubmed
Vitamin D Receptor Gene FOKI Polymorphism Contributes to Increasing the Risk of HIV - Negative Tuberculosis : Evidence from a Meta - Analysis . - pubmed
BACKGROUND : Vitamin D receptor ( VDR ) gene FokI polymorphism have been studied in relation to tuberculosis ( TB ) in many populations and provided inconsistent results . - pubmed
On the first CT reevaluation , the sorafenib response rates were 20 % , 5 % , 7 % and 3 % according to Choi , EASL , mRECIST and RECIST 1.1 . - pubmed
In keratitis - ichthyosis - deafness syndrome , different connexin 26 mutations can either form dominant hemichannels with altered calcium regulation or increased calcium permeability , leading to clinical subtypes of this syndrome . - pubmed
Polyriboinosinic - polyribocytidylic acid ( polyI : C ) , a ligand for toll - like receptor 3 , was selected as a model nucleic acid - based adjuvant . - pubmed
The in vitro degradation behaviors of the bare AZ31 alloy and coated samples were evaluated in a simulated body fluid ( SBF ) using the potentiodynamic polarization curve test and the static immersion test . - pubmed
The in vitro degradation tests showed that the PEO / PCL composite coating improved the corrosion resistance of the AZ31 alloy in SBF with a more positive corrosion potential and a lower corrosion current density . - pubmed
After further surface functionalization by polydopamine , the corrosion resistance of the composite coating was maintained , while its bioactivity was significantly enhanced with a large amount of hydroxyapatite ( HA ) formed on its surface after immersion in SBF . - pubmed
This feasible method for fabricating a cytocompatible and antibacterial composite coating on a biodegradable AZ31 alloy may be promising in implant applications due to the osteointegration and anti - infection properties of these materials post operation . - pubmed
Lipin-1 exhibits a dual function : an enzyme that catalyzes the conversion of phosphatidate to diacylglycerol and a transcriptional regulator . - pubmed
Our data showed that resveratrol significantly increased the levels of Lipin-1 protein and mRNA in HSCs . - pubmed
Further investigation revealed that resveratrol blocked the polyubiquitination of Lipin-1 . - pubmed
Resveratrol inhibited TGF - beta - induced fibrogenic gene expression . - pubmed
TGF - beta - induced SMAD binding element - luciferase reporter activity was significantly diminished by resveratrol with a simultaneous decrease in SMAD3 phosphorylation . - pubmed
Consistently , knockdown of the Lipin-1 gene using siRNA abolished the inhibitory effect of resveratrol . - pubmed
We conclude that Lipin-1 can antagonize HSC activation through the inhibition of TGF - beta / SMAD signaling and that resveratrol may affect Lipin-1 gene induction and contribute to the inhibition of TGF - beta - mediated hepatic fibrogenesis . - pubmed
The most common grade 3 - 4 adverse events in the leucovorin and fluorouracil group were neutropenia without fever ( 17 [ 23 % ] ) , fatigue ( 16 [ 22 % ] ) , paraesthesia ( 14 [ 19 % ] ) , diarrhoea ( nine [ 12 % ] ) , and mucositis ( seven [ 10 % ] ) ; in the gemcitabine group they were neutropenia without fever ( 12 [ 32 % ] ) , thrombocytopenia ( seven [ 18 % ] ) , fatigue ( eight [ 21 % ] ) , anaemia ( five [ 13 % ] ) , increased alanine aminotransferase and aspartate aminotransferase concentrations ( five [ 13 % ] for both ) , and paraesthesia ( four [ 11 % ] ) . - pubmed
Grade 1 - 3 elevation of bilirubin , alkaline phosphatase , aspartate aminotransferase , and alanine transaminase were more frequently recorded in arm A than in arm B. - pubmed
METHODS : PM10 concentrations were recorded and filter paper samples were collected using E - samplers ; PM1 , PM2.5 , black carbon ( BC ) , methane ( CH4 ) , and carbon monoxide ( CO ) were also measured . - pubmed
We analysed NOx ( nitrite+nitrate ) and hydrogen peroxide ( H2O2 ) concentrations , transcript levels of endothelial nitric oxide synthase ( eNOS)/inducible nitric oxide synthase ( iNOS ) and arginine modulators , hydrogen peroxide regulators in the cohort . - pubmed
The transcripts involved in arginine bioavailability : Argininosuccinate lyase ( ASL ) , Solute Carrier Family1 , member 7 ( SLC7A1 ) and Arginase 1 ( ARG1 ) and Asymmetric Dimethyl Arginine ( ADMA ) metabolism : Protein arginine methyltransferase 1 ( PRMT1 ) and Dimethylarginine dimethylaminohydrolase 2 ( DDAH2 ) also showed differential expression . - pubmed
Biomarkers of kidney function were determined , including glomerular filtration rate ( eGFR ) estimated from the Chronic Kidney Disease Epidemiology Collaboration equation , urine albumin - to - creatinine ratio ( ACR ) , microalbuminuria status , and glomerular hyperfiltration status . - pubmed
All the processes are done in a single system ( one - pot and in - situ processing ) based on deep eutectic choline chloride - calcium chloride ( Calcoline ) at mild temperature of 50 C in direct contact with air . - pubmed
About 70 % of the adult world population is lactose - intolerant , due to low levels of intestinal lactase , also called lactase - phlorizin hydrolase ( LPH ) , a beta - d - galactosidase found in the apical surface of the intestinal microvilli . - pubmed
The photocatalytic degradation rates of NCTs were identical with respect to their adsorption capacities , and the apparent rate constant ( kapp ) values indicated that the amount of TiO2 in NCTs played a vital role in the degradation of MEF . - pubmed
, Ac - MtFluNox , Lyso - RhoNox , and ER - SiRhoNox , which can detect Fe ( ii ) specifically in the mitochondria , lysosomes , and endoplasmic reticulum ( ER ) , respectively . - pubmed
The present article reviews various attempts to test drugs approved for different purposes such as calcium channels blockers , non - steroidal anti - inflammatory drugs , cardenolides , antidiabetic , antibiotics and antimalarial for treating retinoblastoma . - pubmed
METHODS : Disc - shaped specimens ( n=15 ; O=10 mm ; thickness=1.0 mm ) were produced from CAD / CAM blocks as follows : feldspathic ( FEL ) ; leucite ( LEU ) ; lithium disilicate ( LD ) ; zirconia - reinforced lithium silicate ( ZRLS ) ; polymer - infiltrated ceramic network ( PICN ) ; and resin nanoceramic ( RNC ) . - pubmed
Hypermethylation of transcription factor activating enhancer - binding protein 2e ( TFAP2E ) has been reported to be associated with chemoresistance to 5 - fluorouracil ( 5 - FU ) in gastric cancer ( GC ) . - pubmed
Collectively , our data support an important role of exosomes and exosomal miRNAs in TFAP2E methylation - induced chemoresistance to 5 - FU in GC . - pubmed
Measurement of ultra - trace level of intact oxytocin in plasma using SALLE combined with nano - LC - MS . - pubmed
In these experiments , spinal nerve ligation ( SNL ) and a daily injection of propentofylline ( 1 mg / kg , ip ) as a glial cell inhibitor agent , 1 h following nerve ligation during 7 - day post - SNL period , were performed . - pubmed
Prednisolone treatment induced temporary factor IX normalization in mild hemophilia B who required an epidural infiltration : A case report . - pubmed
Surprisingly , hemostasis result tests performed before epidural infiltration were a normal activated partial thrombin time at 36.1 s. ( normal range 27.9 - 37.7 s. ) and factor IX ( FIX ) level 76 % ( normal range>70 % ) , 13 days after prednisolone introduction . - pubmed
By using both in vivo and ex vivo approaches , we have found that pharmacological doses of progesterone ( P4 ) and estradiol ( E2 ) produced an inhibition in the expression of hypothalamic GnRH , while physiological doses produced a differential effect on the pulsatile release frequency or genomic expression of GnRH . - pubmed
Our ex vivo experiment indicates that a short - term effect of E2 modulates the frequency of GnRH release pattern that would be associated with membrane ERs . - pubmed
On the other hand , our in vivo approach suggests that a long - term effect of E2 , acting through the classical nuclear ERs - PRs pathway , would produce the modification of GnRH mRNA expression during the GnRH pre - ovulatory surge . - pubmed
This paper describes the fabrication of an imprinted fluorescent nanoprobe based on SiO2 - coated NaYF4 : Yb , Er upconversion nanoparticles ( UCNP ) encapsulated with a molecularly imprinted polymer ( MIP ) for determination of acetamiprid . - pubmed
The fluorescent MIP nanoprobe was prepared using UCNP as the material for fluorescence signal readout , acetamiprid as template molecule , methylacrylic acid ( MAA ) as functional monomer , and ethyleneglycol dimethacrylate ( EGDMA ) as cross - linking agent . - pubmed
UCNP@MIP shows a high selectivity towards acetamiprid with an imprinting factor ( IF ) of 7.84 . - pubmed
Graphical abstractA simple fluorescence nanoprobe which integrates upconversion nanoparticles ( UCNPs ) and molecular imprinting polymer ( MIP ) was developed for the determination of acetamiprid . - pubmed
Gene panel test showed c.697A > G heterozygous variant in the PSEN1 gene leading to a M233V amino acid change , which was validated by a Sanger sequencing . - pubmed
In published O - glycoproteomics data , our method more than doubled the number of glycoPSMs annotated when searching the same glycans as the original search , and yielded 4- to 6 - fold increases when expanding searches to include additional glycan compositions and other modifications . - pubmed
In this paper , we report on our simple innovative approach in the selection of best - transfected cells with the highest expression of ASPH using subclone selection , quantitative real - time polymerase chain reaction , and gradual increment of hygromycin concentration . - pubmed
Furthermore , the gradual increment of hygromycin ( up to 400mg / mL ) improved the expression of ASPH . - pubmed
Succinic acid inhibits the activity of cytochrome P450 ( CYP450 ) enzymes . - pubmed
OBJECTIVE : The effect of succinic acid on the activity of cytochrome P450 ( CYP450 ) enzymes was investigated in this study . - pubmed
MATERIALS AND METHODS : The effect of succinic acid ( 100 muM ) on the activity of eight isoforms of CYP450 ( i.e. - pubmed
RESULTS : Succinic acid exerted inhibitory effect on the activity of CYP3A4 , 2D6 , and 2C9 with the IC50 values of 12.82 , 14.53 , and 19.60 muM , respectively . - pubmed
Succinic acid inhibited the activity of CYP3A4 in a non - competitive manner with the Ki value of 6.18 muM , and inhibited CYP2D6 and 2C9 competitively with Ki values of 7.40 and 9.48 muM , respectively . - pubmed
DISCUSSION AND CONCLUSIONS : Succinic acid showed in vitro inhibitory effects on the activity of CYP3A4 , 2D6 , and 2C9 , which indicated the potential drug - drug interactions . - pubmed
Succinic acid should be carefully co - administrated with the drugs metabolized by CYP3A4 , 2D6 , and 2C9 . - pubmed
This paper demonstrates that the molecular conformation ( in addition to the composition and structure ) of molecules making up self - assembled monolayers ( SAMs ) influences the rates of charge tunneling ( CT ) through them , in molecular junctions of the form AuTS / S ( CH2 ) 2CONR1R2//Ga2O3 / EGaIn , where R1 and R2 are alkyl chains of different length . - pubmed
tunneling barrier width ) and their rectification ratios at + -1.0 V. When R1 = H , the molecules are well ordered and exist predominantly in trans - extended conformations . - pubmed
As shown previously for heterogeneous nuclear RNA ( HnRNA ) and 45 S precursor ribosomal RNA ( pre - rRNA ) , proflavine immediately stabilizes newly synthesized adenovirus RNA , while ethidium bromide stabilizes the RNA after a 30 - -60 min lag period . - pubmed
Partial isolation and translation in vitro of messenger ribonucleic acid for phosphoenolpyruvate carboxykinase ( guanosine triphosphate ) . - pubmed
The presence of newly synthesized phosphoenolpyruvate carboxykinase ( GTP ) was detected by immunoprecipitation with a specific antibody to the enzyme and analysis by sodium dodecyl sulphate / polyacrylamide - gel electrophoresis . - pubmed
The optimal concentrations of magnesium acetate and KCl for phosphoenolpyruvate carboxykinase synthesis were determined . - pubmed
When polyribosomal RNA was separated by sucrose - gradient centrifugation , phosphoenolpyruvate carboxykinase mRNA migrated between 20 and 26 S in keeping with the high mol . - pubmed
Thymidine kinase was eluted with 0.1 mM thymidine , 0.1 M KC1 in 0.01 M Tris - HC1 , pH 7.5 . - pubmed
The purified enzyme accounted for about 26 % of the applied activity ; the specific activity of the purified material ( peak fraction ) was 3,500 moles TMP formed per mg protein per 10 min . - pubmed
There was , however , no correlation between the PRA levels and the blood pressure decrease after salt depletion or after propranolol . - pubmed
Proceedings : Decrease in the accuracy of DNA polymerase following treatment with gamma - rays and methylnitrosourea . - pubmed
The results suggest that suppression of basal DA release in the NAC is an adaptive consequence of sustained cocaine exposure and may in part underlie the post - cocaine anhedonia observed in behavioral models of cocaine withdrawal . - pubmed
Essential amino acids like methionine , cystine , threonine and valine were found to be limiting in the seed proteins of both the germplasms . - pubmed
Antinutritional substances like total free phenols , tannins , L - DOPA , trypsin inhibitor activity and phytohaemagglutinating activity also were investigated . - pubmed
In the presence of Mo , PWM stimulation of T lymphocytes ( highly depleted of B lymphocytes ) induces as much IL-2 mRNA as phytohemagglutinin ( PHA ) , but results in higher and persistent IL-2 levels in culture supernatants despite the concomitant T cell mitogenesis , suggesting that PWM - activated T cells do not utilize the IL-2 they produce . - pubmed
Moreover , the PWM - induced T cell mitogenesis was not inhibited by anti - IL-2 or CD25 antibodies and only partially ( 50%-60 % ) inhibited by cyclosporin A , while these treatments abrogated the PHA - induced one . - pubmed
In addition , peptide YY ( 1 mumol / L ) reduced by 42 % + /- 6 % electrically stimulated muscle contractions that were blocked by atropine and tetrodotoxin , providing additional evidence that the peptide inhibits release of acetylcholine . - pubmed
Next , the effect of peptide YY on potassium - evoked release of [ 3H ] acetylcholine and whether the peptide inhibits cyclic adenosine monophosphate - dependent release of acetylcholine were examined . - pubmed
In addition , peptide YY reduced half - maximal forskolin and cholera toxin - evoked release of acetylcholine by 45 % + /- 6 % and 42 % + /- 8 % , respectively , suggesting that the peptide can inhibit cyclic adenosine monophosphate - dependent release of acetylcholine . - pubmed
In addition , peptide YY antagonized cyclic adenosine monophosphate - mediated release of acetylcholine through a pertussis toxin - sensitive mechanism . - pubmed
During continuous exposure to TEA , a significant increase in PRL secretion occurred by 20 min and the response was sustained until the end of a 60 - min exposure . - pubmed
Blocking Ca2 + influx by employing a Ca ( 2 +)-depleted medium or the Ca2 + channel blocker , nifedipine , prevented induction of PRL secretion by 20 mM TEA . - pubmed
Preincubation of the cells for 10 min with 20 mM TEA did not inhibit PRL secretion induced by thyrotropin - releasing hormone ( TRH ) , phorbol 12 - myristate 13 - acetate ( TPA ) or by cell swelling produced by 30 % medium hyposmolarity , but significantly depressed that induced by depolarizing 30 mM K + . - pubmed
BaCl2 , another K + channel blocker , had the same effect on PRL secretion as TEA . - pubmed
Molecular basis of androgen resistance in a family with a qualitative abnormality of the androgen receptor and responsive to high - dose androgen therapy . - pubmed
The androgen receptor gene in this family contains two structural alterations : a single nucleotide substitution at position 2444 in exon 5 ( adenosine----guanosine ) that converts tyrosine 761 to a cysteine residue and a shortened glutamine homopolymeric segment in exon 1 that encodes 12 rather than the usual 20 - 22 glutamines . - pubmed
A family study was performed using polymerase chain reaction amplification of the glutamine - rich segment , and it was shown that the sister of the proband does not carry the mutant allele . - pubmed
Marked thermolability of the transfected receptor protein , however , was demonstrable only upon introduction of an androgen receptor cDNA containing both the partial deletion of the glutamine homopolymeric segment and a cysteine residue at position 761 . - pubmed
Specific stages of the prereplicative G1 phase of the cell cycle in nonneoplastic cells requires extracellular Ca2 + for successful transition . - pubmed
One of these agonist , inositol 1,3,4,5 - tetrakisphosphate [ Ins ( 1,3,4,5 ) P4 ] , has been demonstrated by us to also stimulate the activity of a phosphoprotein phosphatase . - pubmed
A patch - clamp study of excitatory amino acid activated channels . - pubmed
In contrast , dexamethasone was found to inhibit the induction of transin mRNA when added with , or following , NGF treatment . - pubmed
Finally , we show that sequences contained within 750 bp of the 5 ' untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone . - pubmed
The upper 95th percentile for the difference between two yearly measurements , expressed as a percent of the population mean , ranged from 4 % for Na + to approximately 20 % for total cholesterol and to greater than 90 % for ferritin . - pubmed
Year - to - year differences attributable to the biological component ranged from a low of 2 % of the population mean for Na+ to 70 % for gamma - glutamyltransferase . - pubmed
A different hydrosmotic response for oxytocin and isoproterenol ( but not for A II ) was found between four cutaneous regions of toad body . - pubmed
The configuration of M13 bacteriophage coat protein in a model membrane was analyzed using protease digestion followed by gel permeation chromatography on Fractogel TSK in formic acid / ethanol . - pubmed
A new reconstitution was thereby developed which involves adding a small volume of a concentrated solution of cholate - solubilized coat protein to preformed vesicles ( with the amount of detergent added being less than that required to solubilize the vesicles ) , freezing in liquid nitrogen , thawing , followed by dialysis to remove excess detergent . - pubmed
Cleavage of transferrin by cyanogen bromide . - pubmed
We show that : ( 1 ) Epinephrine in the 10(-7)-10(-3 ) M concentration range ( ED50 = 11.10(-6 ) M ) inhibits VIP - induced cyclic AMP rise in isolated colonic epithelial cells ; the maximal inhibition reaches 30 % of VIP effect ; epinephrine alters the efficacy of the peptide and does not modify its potency ; epinephrine also reduces the basal cyclic AMP level . - pubmed
( 3 ) The inhibition of VIP action by epinephrine is reversed by the alpha antagonists dihydroergotamine , phentolamine and the alpha 2 antagonist yohimbine , while unaffected by the beta antagonist propranolol and the alpha 1 antagonist prazosin , ( 4 ) Epinephrine inhibits VIP - stimulated adenylate cyclase activity in preparations of colonic plasma membranes . - pubmed
After immunization with the primary test antigen haemocyanin , a decreased antigen specific humoral immune response was observed for all three Ig classes , irrespective of disease activity or the use of corticosteroids . - pubmed
Capsaicin and substance P produced similar excitatory effects in both quiescent and rhythmically contracting bladders , i.e. - pubmed
Atropine pretreatment prevented the neurogenic component of the excitatory effect of both capsaicin and substance P. Repeated applications of capsaicin but not of substance P led to desensitization . - pubmed
Experiments have been carried out in the presence of indomethacin ( IN ) , specific inhibitor of prostaglandin - synthetase . - pubmed
Lower temperature and orthophosphate control of alkaline phosphatase in a cold - requiring plant . - pubmed
The elevated activity of alkaline phosphatase observed in roots and shoots of Verbascum rosettes grown in vitro at 15 degrees C may be the result of a reduced supply of orthophosphate . - pubmed
The rosette reveals greater activities of this enzyme under conditions of lower external phosphate or chilling temperature . - pubmed
The stimulation of phosphatase in tissues having lower levels of phosphate is thus a possible feature of other plant species which also display freezing tolerance . - pubmed
There were three major findings in the various combinations of CBD , HND , and SA in ponies during treadmill exercise . - pubmed
First , the combination of CBD with HND or SA resulted generally in an accentuation of the hypocapnia during exercise that was predictable on the basis of CBD alone . - pubmed
However , in one pony that showed a hypercapnic exercise response after SA alone , CBD subsequently caused a greater exercise hypercapnia . - pubmed
O2 binding properties of the separated aggregating and non - aggregating Hbs purified at low pH can not account for the functional properties of the composite hemolysate , suggesting the presence of other subunits interactions . - pubmed
glutinosa Hb , where the presence of three major monomeric Hbs and a possible CO2 - dependent aggregation had been reported . - pubmed
At the end of reperfusion , bile production , lactate dehydrogenase ( LDH ) secretion , and tissue adenosine triphosphate ( ATP ) was measured . - pubmed
Viability of the group B livers , assessed by bile production , tissue ATP value , and LDH release , was preserved better than that of group A livers . - pubmed
The phenotype of mis5 is DNA replication defect and hypersensitivity to UV ray and hydroxyurea . - pubmed
Since an increase in cytoplasmic free Ca2 + concentration ( [ Ca2+]i ) in the pancreatic beta cell is the major stimulus for insulin release , this study was undertaken to investigate the dynamics of electrical activity , [ Ca2 +]i - changes and insulin release , in stimulated islets from subjects of varying glucose tolerance . - pubmed
Antidepressant / anxiolytic ipsapirone inhibits cold - induced hypothalamic TRH release . - pubmed
Acute administration of the serotonin ( 5 - HT ) 1A receptor agonist ipsapirone has been previously shown to elicit a dose - dependent decrease of cold - elicited thyrotropin ( TSH ) secretion . - pubmed
Therefore , the aim of the present study was to evaluate , by means of a push - pull cannula implanted in the median eminence ( ME ) , whether ipsapirone acts primarily through an inhibition of cold - elicited thyrotropin - releasing hormone ( TRH ) release . - pubmed
1 - Aminocyclopentane - trans-1S,3R - dicarboxylic acid ( 1S,3R - ACPD ) , a compound which selectively activates a metabotropic glutamate receptor ( mGluR ) , suppresses hippocampal excitatory synaptic transmission . - pubmed
This depression is not antagonizable by L-2 - amino-3 - phosphonoproprionic acid ( L - AP3 ) , an agent which counteracts mGluR - induced phosphoinositide ( PI ) metabolism in several neuronal systems . - pubmed
Therefore , we believe that 1S,3R - ACPD activates a novel hippocampal mGluR subtype that is either insensitive to L - AP3 blockade and/or linked to an effector system that does not involve PI turnover . - pubmed
Recently , hormone receptor status ( estrogen [ ER ] , progesterone [ PR ] ) in endometrial cancer has been suggested to be a prognostic factor . - pubmed
Release of tritiated water into the medium was thereupon detected utilizing its lack of binding to active charcoal , which is an established technique to measure in situ thymidylate synthase activity . - pubmed
Like water , dihydrouracil does not bind to active charcoal , and its presence in the cell medium can result in an overestimation of the in situ thymidylate synthase activity . - pubmed
The catabolism of dCyd was highest in macrophages where 25 % of the added dCyd ( 0.5 microM , 0.5 nmol / million cells ) had been converted to dihydrouracil after 30 min , and 90 % after 12 h. The in situ thymidylate synthase activity was found to be the highest in stimulated lymphocytes . - pubmed
This study investigated whether the circadian regulation of luteinizing hormone ( LH ) release may be through direct input of the suprachiasmatic nucleus ( SCN ) to estrogen receptor ( ER)- and/or gonadotropin releasing hormone ( GnRH)-immunoreactive neurons . - pubmed
Results suggest that the circadian system could regulate the timing of the LH surge via two pathways , through input to GnRH and to ER cells . - pubmed
Overexpression of LAS1 results in extra cell surface projections in the mother cell , alterations in actin and SPA2 localization , and the accumulation of electron - dense structures along the periphery of both the mother cell and the bud . - pubmed
Effect of a novel 5 - hydroxytryptamine3 ( 5 - HT3 ) receptor antagonist , GK-128 , on 5 - HT3 receptors mediating contractions and relaxations in guinea - pig distal colon . - pubmed
We investigated 5 - hydroxytryptamine3 ( 5 - HT3 ) receptor - mediating contractions and relaxations in the guinea - pig isolated distal colon using various 5 - HT3 receptor agonists and antagonists , including GK-128 ( 2 -[( 2 - methylimidazol-1 - yl ) methyl ] benzo [ f ] thiochromen-1 - one monohydrochloride hemihydrate ) . - pubmed
Selective 5 - HT3 receptor agonists , 2 - methyl-5 - HT and m - chlorophenylbiguanide , produced spantide - insensitive contraction and atropine - insensitive contraction and the relaxation . - pubmed
Other selective 5 - HT3 receptor antagonists , azasetron and tropisetron , also exhibited higher affinity in contraction than in relaxation , but the extent of their affinity differences was smaller than that observed in GK-128 . - pubmed
These results suggest that the 5 - HT3 receptors which mediate contraction and relaxation in the guinea - pig distal colon may not be the same , and that GK-128 is a 5 - HT3 receptor antagonist with a stronger potency for contraction . - pubmed
Lower - efficacy D1 or D2 agonists substituted for methamphetamine either partially ( SDZ 208 - 911 ) or not at all ( SKF 77434 , SDZ 208 - 912 ) . - pubmed
Pretreatment with dopamine receptor blockers [ D1 ( SCH 39166 , 0.1 mg / kg ) or D2 ( remoxipride , 3.0 mg / kg and nemonapride , 0.003 mg / kg ) ] and low - efficacy agonists [ D1 ( SKF 77434 ; 3.0 mg / kg ) or D2 ( SDZ 208 - 911 and SDZ 208 - 912 ; 0.01 - 0.03 mg / kg ) ] antagonized the discriminative - stimulus effects of methamphetamine . - pubmed
These results support the view that both dopaminergic D1 and D2 mechanisms mediate the discriminative - stimulus effects of methamphetamine ; further , they indicate that selected dopamine D1 partial agonists may have antagonist actions at doses that do not produce undesirable effects associated with dopamine receptor blockade . - pubmed
In this study , we conducted a nested reverse transcription - polymerase chain reaction ( RT - PCR)-based assay to detect the TLS / FUS - CHOP fusion gene transcripts using archival formalin - fixed , paraffin - embedded tumor specimens . - pubmed
The present study confirms previous data about the effects of Zn supplementation on aging processes and demonstrates that HF - MRI is a powerful technique to study processes of aging , providing information about the effects of drug treatments on these processes . - pubmed
Kemptide and autophosphorylation activities of PrKX are strongly inhibited by the RIalpha subunit and by protein kinase inhibitor in vitro , but only weakly by the RIIalpha subunit . - pubmed
The inhibition by the RIalpha subunit is reversed by addition of nanomolar concentrations of cAMP ( Ka = 40 nM ) , thus demonstrating that PrKX is a novel , type I cAMP - dependent protein kinase that is activated at lower cAMP concentrations than the holoenzyme with the Calpha subunit of cAMP - dependent protein kinase . - pubmed
Microinjection data clearly indicate that the type I R subunit but not type II binds to PrKX in vivo , preventing the translocation of PrKX to the nucleus in the absence of cAMP . - pubmed
The RIIalpha subunit is an excellent substrate for PrKX and is phosphorylated in vitro in a cAMP - independent manner . - pubmed
We discuss how PrKX can modulate the cAMP - mediated signal transduction pathway by preferential binding to the RIalpha subunit and by phosphorylating the RIIalpha subunit in the absence of cAMP . - pubmed
NOEs , chemical shift perturbations and temperature coefficients of amide protons in aqueous and nonaqueous media suggest that carbohydrate moiety in threonine glycosylated peptides ( series II ) is in close proximity to the peptide backbone . - pubmed
Delineation of solution structure of these ( glyco)peptides by NMR and CD revealed largely a poly L - proline type II and/or random coil conformation for the peptide core . - pubmed
Typical peptide fragments of tandem repeat sequence of mucin ( MUC7 ) showing profound glycosylation effects and distinct differences between serine and threonine glycosylation as observed in the present investigation could serve as template for further studies to understand the multifunctional role played by mucin glycoproteins . - pubmed
IntI1 protein , as well as IntI1 - COOH , a truncated form containing its carboxy - terminal domain , has been purified . - pubmed
IntI1 - COOH did not bind either attI or attC , indicating that the N - terminal domain of IntI1 was required for binding to double - stranded attI. - pubmed
Protein IntI1 - COOH bound ss attC as well as did the complete integrase , indicating that the ability of the protein to bind ss aadA1 attC was contained in the region between amino acids 109 and 337 of IntI1 . - pubmed
Binding to ss attI DNA by the integrase , but not by IntI1 - COOH , was also observed and was specific for the attI bottom strand , indicating similar capabilities of IntI1 for binding attI DNA in either double - stranded or ss conformation . - pubmed
Insulin - sensitivity index and carbohydrate and lipid metabolism in gestational diabetes . - pubmed
The insulin - tolerance test ( ITT ) consisted of an intravenous bolus of 0.1 IU / kg of regular insulin with glucose sampling at -5 , 0 , 3 , 5 , 7 , 10 and 15 minutes . - pubmed
Characterization of a phospholipase C beta 2 - binding site near the amino - terminal coiled - coil of G protein beta gamma subunits . - pubmed
273 , 7148 - 7154 ) , we showed that overlapping peptides , N20 K ( Asn(564)-Lys(583 ) ) and E20 K ( Glu(574)-Lys(593 ) ) , from the catalytic domain of phospholipase C ( PLC ) beta2 block Gbetagamma - dependent activation of PLC beta2 . - pubmed
The ability of the PLC beta2 - derived peptide to cross - link to each betagamma mutant was then analyzed to identify the reactive sulfhydryl moiety on the beta subunit required for the cross - linking reaction . - pubmed
This indicates that the peptide is specifically binding to a region near cysteine 25 of beta ( 1 ) which is located in the amino - terminal coiled - coil region of beta ( 1 ) and identifies a PLC - binding site distinct from the alpha subunit interaction site . - pubmed
The sequence of the lens protein ' s cDNA ( about 1.7 kb total ) described an open reading frame of 582 residues which coded for a protein of 194 amino acids . - pubmed
Modulation of radiation - induced protein kinase C activity by phenolics . - pubmed
Curcumin , ellagic acid and quercetin were effective in inhibiting radiation - induced PKC activity . - pubmed
Curcumin and ellagic acid were found to be more inhibitory towards radiation - induced PKC activity , while quercetin was the least effective . - pubmed
Curcumin was found to inhibit the activated cytosolic and particulate PKC at very low concentrations . - pubmed
Suppression of PKC activity by phenolics may be one of the means of preventing the development of radioresistance following radiotherapy . - pubmed
Eleven different PrP genotypes encoding at least five unique predicted mature PrP amino acid sequences were found . - pubmed
History , physical examination , karyotype , FSH , LH , testosterone , testis histology and results of ICSI using ejaculated or testis sperm were retrospectively accumulated in two academic clinical practices . - pubmed
FSH , LH , testosterone and testis histology could not differentiate those with oligospermia or azoospermia , nor could they predict whether sperm could be found in harvested testis tissue . - pubmed
CGs had plasma catecholamine levels sampled at rest and in response to a stressor ( speech task ) before and 1 month after a 2 - week in - home respite intervention . - pubmed
Multidrug - resistant ( MDR ) counterparts of these cell lines express differentially the MDR - conferring ATP - binding cassette transporter genes MDR1 , MRP1 and BCRP , respectively . - pubmed
The N- and C - terminal amino acids of human hemoglobin H. - pubmed
Linear alkylbenzene sulfonate and boron monitoring in four catchments in the UK contribution to GREAT - ER # 11 . - pubmed
3 - OH flavone inhibition of epidermal growth factor - induced proliferaton through blocking prostaglandin E2 production . - pubmed
Epidermal growth factor ( EGF ) has been shown to induce proliferation in cells , however , the role of prostaglandin E ( 2 ) ( PGE ( 2 ) ) plays in EGF - induced proliferation in still unclear . - pubmed
PD98059 , a specific ERK1 / 2 inhibitor , inhibited EGF - induced proliferation with concomitant decreases in ERK1 / 2 phosphorylation and COX-2 / PGE ( 2 ) induction . - pubmed
Non - steroid anti - inflammatory drugs ( NSAIDs ) such as aspirin and diclofenac , a COX activity inhibitor , inhibited EGF - induced proliferation by blocking PGE ( 2 ) production . - pubmed
The addition of PGE ( 2 ) reversed the inhibitory effects of PD98059 , aspirin , and diclofenac on EGF - induced proliferation . - pubmed
This suggests that COX-2 / PGE ( 2 ) activation involves in EGF - induced proliferation and locates at the downstream of ERK1 / 2 activation . - pubmed
Furthermore , the natural product , 3 - OH flavone , showed the most - potent inhibitory activity on EGF - induced proliferation among 9 structurally - related compounds , and suppression of EGF receptor phosphorylation , ERK1 / 2 phosphorylation , and COX-2 / PGE ( 2 ) production by 3 - OH flavone was identified . - pubmed
PGE ( 2 ) addition attenuates the inhibitory activity of 3 - OH flavone on EGF - induced proliferation by MTT assay and colony formation by soft agar assay . - pubmed
Results of our present study provide evidence to demonstrate that PGE ( 2 ) is an important downstream molecule in EGF - induced proliferation , and 3 - OH flavone , which inhibits PGE ( 2 ) production by blocking MAPK cascade , might reserve potential for development as an anti - cancer drug . - pubmed
Student 's t test , Tukey test and repeated measures variance analysis were used to evaluate differences in the study group and between the women with polycystic ovary syndrome ( PCOS ) and idiopathic hirsutism after gonadotropin releasing hormone ( GnRH)-agonist administration . - pubmed
The redox reaction of cytochrome c immobilized on the bare surfaces of microelectrodes and macroscopic electrodes ( macroelectrodes ) composed of different planes of highly oriented pyrolytic graphite has been investigated using cyclic voltammetry . - pubmed
Markers of inflammation ( macrophage , lactate dehydrogenase , nitric oxide ) were apparently more in the BAL fluid of those with lung involvement than in those without , though the arsenic content did not differ significantly . - pubmed
In SOCS-3 knockdown studies , antisense oligonucleotides inhibited the expression of SOCS-3 and increased the Ang II - induced STAT activation and c - Fos / c - Jun expression , then resulting in a more severe renal damage . - pubmed
The aqueous extracts of them contained the common seleno compound that was identified as gamma - glutamylmethylselenocysteine by an electrospray ionization - tandem mass spectrometry ( ESI - MS / MS ) . - pubmed
The role of " premature " coronary death and the level of some risk factors , particularly obesity and blood pressure in African descendants and high triglycerides , low high - density lipoproteins , and diabetes in some Hispanics , is higher than in whites . - pubmed
In addition , we examined the ability of trimipramine ; a tricyclic antidepressant that is devoid of monoamine reuptake inhibitory properties on cytokine production . - pubmed
All of the antidepressants suppressed IFN - gamma production in the 10 - 50 microM concentration range , irrespective of their preference for serotonin or noradrenaline transporters . - pubmed
The fact that trimipramine also suppressed IFN - gamma production and T - cell proliferation indicates that these immunomodulatory actions of antidepressants are most likely unrelated to inhibition of monoamine reuptake . - pubmed
Interestingly , exposure to a lower concentration ( 1 microM ) of the antidepressants tended to increase T - cell - derived IL-10 production , with significant effects elicited by the noradrenaline reuptake inhibitors reboxetine and desipramine . - pubmed
After an 18 - hour preincubation in nitrogen - free medium the accumulation ratio of AIB exceeded 4.5 at an external AIB concentration of 10 micromolar . - pubmed
METHODS : The quinolone resistance determining regions ( QRDRs ) of gyrA , gyrB , parC and parE of these strains were analysed by PCR - based sequencing . - pubmed
RESULTS : All 18 strains had at least one amino acid substitution in the QRDRs of GyrA and ParC , which included Ser-81 - ->Tyr / Phe and Glu-85 - ->Lys in GyrA and Ser-79 - ->Phe / Ile / Tyr , Asp-83 - ->Tyr , Asn-91 - ->Asp , Ser-107 - ->Phe , Lys-137 - ->Asn and Ala-142 - ->Ser in ParC. - pubmed
Most isolates had Asp-435 - ->Asn / Ile-460 - ->Val / Ala-596 - ->Thr substitutions in ParE , while no amino acid substitution in GyrB was noted in all isolates . - pubmed
Ten isolates for which levofloxacin MICs were 16 or 32 mg / L had multiple mutations in both GyrA and ParC. - pubmed
We now report the discovery of an alternative and quantitatively dominant mechanism for cyanate formation and protein carbamylation at sites of inflammation and atherosclerotic plaque : myeloperoxidase - catalyzed oxidation of thiocyanate , an anion abundant in blood whose levels are elevated in smokers . - pubmed
We also show that myeloperoxidase - catalyzed lipoprotein carbamylation facilitates multiple pro - atherosclerotic activities , including conversion of low - density lipoprotein into a ligand for macrophage scavenger receptor A1 recognition , cholesterol accumulation and foam - cell formation . - pubmed
Does the activity of CuZnSOD change due to fluoxetine treatment ? - pubmed
The activity of CuZnSOD rises after fluoxetine treatment . - pubmed
New biological roles could be attributed to various TCSs , including roles in cytochrome c biogenesis ( HssRS ) , transport of carbohydrates , peptides and/or amino acids ( YkoGH , LytSR ) , and resistance to toxic ions ( LiaSR ) , antimicrobial peptides ( BceRS ) and beta - lactam antibiotics ( BacRS , YcbLM ) . - pubmed
At higher pH chlorin p6 was found to be photodynamically more effective in aqueous environment whereas at lower pH chlorin p6 was photodynamically more effective in hydrophobic environment . - pubmed
One - pot [ 1 + 1 + 1 ] synthesis of dithieno [ 2,3 - b : 3 ' , 2 '- d ] thiophene ( DTT ) and their functionalized derivatives for organic thin - film transistors . - pubmed
A facile one - pot [ 1 + 1 + 1 ] synthesis of dithieno [ 2,3 - b : 3 ' , 2 '- d ] thiophene (; ) has been achieved , enabling the efficient realization of a new DTT - based semiconductor series for organic thin - film transistors ( OTFTs ) . - pubmed
When " ARDS - controlled window " appeared , the ventilation mobility was switched to oronasal face continuous positive airway pressure ( CPAP ) , followed by CPAP until weaning ; the control group was weaned with SIMV+PSV+PEEP . - pubmed
This study was carried out to develop and optimize oral sustained - release formulations for Ambroxol hydrochloride matrix pellets using a combination of wax and water - insoluble polymer , glyceryl behenate ( Compritol 888 ATO ) and Ethylcellulose ( EC(7 FP ) ) . - pubmed
It involved three factors : the content of Compritol 888 ATO ( X(1 ) ) , EC(7 FP ) ( X(2 ) ) , and the matrix formation methods ( X(3 ) ) , as independent variables . - pubmed
Medroxyprogesterone acetate also caused glomerular , vascular , tubular , and interstitial lesions that were accompanied by increased blood pressure and enhanced NADPH oxidase ( p67phox ) and sodium channel ( alpha - ENaC ) expression . - pubmed
Drospirenone and spironolactone also increased renal angiotensin II type 2 receptor expression and relieved aldosterone - induced suppression of serum angiotensin II levels . - pubmed
Evaluation of selective gamma - secretase inhibitor PF-03084014 for its antitumor efficacy and gastrointestinal safety to guide optimal clinical trial design . - pubmed
We describe the potent antitumor effects of PF-03084014 , a small molecule that is a reversible , noncompetitive , and selective gamma - secretase inhibitor . - pubmed
PF-03084014 treatment also resulted in robust NICD reduction in HBP - ALL xenograft models . - pubmed
Collectively , the results show that inhibition of Notch signaling by PF-03084014 while minimizing gastrointestinal toxicity presents a promising approach for development of therapies for Notch receptor - dependent cancers . - pubmed
Using immunohistochemical techniques , we found that GABA is colocalized with the vesicle - associated protein synapsin in nearby nerves and hence is presumably released there . - pubmed
Cycling hypoxia - induced HIF-1alpha activation reflected ROS mediated HIF-1alpha synthesis and stabilization , whereas non - interrupted hypoxia - induced HIF-1alpha activation was due to decreased HIF-1alpha degradation resulting from decreased prolyl hydroxylation . - pubmed
The instrument employs a microchip - based nano - LC column packed with porous graphitized carbon ( PGC ) to provide excellent isomer separation for SHMOs with highly reproducible retention time . - pubmed
In previous studies , low blood levels of n-3 fatty acids ( FA ) have been associated with increased risk of cardiac death , and the omega-3 index ( red blood cell ( RBC ) eicosapentaenoic acid ( EPA ) and docosahexaenoic acid ( DHA ) expressed as weight percentage of total FA ) has recently been proposed as a new risk factor for death from coronary artery disease , especially following sudden cardiac arrest ( SCA ) . - pubmed
We could not demonstrate significant changes in the FA measurements in any of the groups , this also applied to the omega-6 / omega-3 ratio and the arachidonic acid ( AA)/EPA ratio . - pubmed
GSH is synthesized into two enzymatic steps , the first , and the rate - limiting one , is catalyzed by glutamate - cysteine ligase ( GCL ) to form a dipeptide which is then converted to GSH by GSH synthetase . - pubmed
In this chapter , we describe assays for the measurements of GSH levels and GCL activity . - pubmed
Synthesis , detailed structural characterization ( X - ray , NMR , MS , IR , elemental analysis ) , and studies of toxicity , antioxidant activity and bioavailability of unique potent anti - atherosclerotic succinobucol - steroid conjugates are reported . - pubmed
A series of pyrimidodiazepines was identified as potent Polo - like kinase 1 ( PLK1 ) inhibitors . - pubmed
The lead compound 7 ( RO3280 ) has potent inhibitory activity against PLK1 , good selectivity against other kinases , and excellent in vitro cellular potency . - pubmed
Cyclin - dependent kinase 7 / 9 inhibitor SNS-032 abrogates FIP1 - like-1 platelet - derived growth factor receptor alpha and bcr - abl oncogene addiction in malignant hematologic cells . - pubmed
PURPOSE : The " gate - keeper " mutations T674I platelet - derived growth factor receptor alpha ( PDGFRalpha ) in hypereosinophilic syndrome ( HES ) and T315I Bcr - Abl in chronic myeloid leukemia ( CML ) are resistant to imatinib and the second - generation small - molecule tyrosine kinase inhibitors ( TKI ) . - pubmed
The purpose of this study was to evaluate the strategy of shutting down the transcription and expression of FIP1 - like-1 ( FIP1L1)-PDGFRalpha and Bcr - Abl with SNS-032 , an inhibitor of cyclin - dependent kinase 7 ( CDK7 ) and CDK9 in phase I clinical trials . - pubmed
EXPERIMENTAL DESIGN : The effects of SNS-032 on PDGFRalpha and Bcr - Abl signaling pathways , apoptosis , and cell cycling were analyzed in TKI - resistant cells of HES and CML . - pubmed
RESULTS : SNS-032 inhibited the phosphorylation on Ser5 and Ser2 of RNA polymerase II . - pubmed
SNS-032 decreased both the mRNA and protein levels of FIP1L1 - PDGFRalpha and Bcr - Abl and inhibited the proliferation of malignant cells expressing FIP1L1 - PDGFRalpha or Bcr - Abl . - pubmed
CONCLUSIONS : This CDK7 / 9 inhibitor potently inhibits FIP1L1 - PDGFRalpha - positive HES cells and Bcr - Abl - positive CML cells regardless of their sensitivity to imatinib . - pubmed
Erufosine suppresses breast cancer in vitro and in vivo for its activity on PI3 K , c - Raf and Akt proteins . - pubmed
PURPOSE : This study investigated the antineoplastic effect of the membrane active alkylphosphocholine erufosine in breast carcinoma models in vitro and in vivo and determined its influence on the PI3K / Akt and Ras / Raf / MAPK signaling pathways . - pubmed
In addition , erufosine caused dose - dependent decreases in the phosphorylation of PI3 K ( p85 ) , Akt ( PKB ) at Thr 308 and cRaf in both cell lines . - pubmed
In addition , it can be derived that the mechanism of action of erufosine involves influence on both , PI3K / Akt and Ras / Raf / MAPK signaling pathways . - pubmed
Voltage - gated proton channels are expressed in microglia , and are required for NADPH oxidase - dependent reactive oxygen species generation . - pubmed
In addition , the EGCG effect was preserved in the presence of the cytoskeletal stabilizers paclitaxel and phalloidin , phosphatase inhibitors , the antioxidant Trolox , superoxide dismutase or catalase . - pubmed
Spinal cord involvement secondary to non - Hodgkin ' s lymphoma identified by 18F - FDG PET / CT . - pubmed
Here , we used a tetracycline regulated d2EGFP reporter construct containing specific 3'UTR sequences from DDB2 to identify novel cis - acting elements that regulate mRNA stability . - pubmed
Conversely , these same 3'UTRs led to more rapid induction of the reporter mRNA , export of the message to the cytoplasm and the subsequent accumulation of the encoded reporter protein , indicating that this newly identified cis - acting element affects transcriptional and post - transciptional processes . - pubmed
After nitrogen starvation , green fluorescent protein ( GFP)-Syb1 is found in intracellular dots . - pubmed
Electron microscopy shows that an abnormal spore wall is often formed in syb1 - S1 mutant spores . - pubmed
Although most syb1 - S1 mutant spores are germinated , they are less tolerant to ethanol than wild - type spores . - pubmed
The syb1 - S1 allele carries a missense mutation , resulting in replacement of a conserved cysteine residue adjacent to the transmembrane domain , which reduces the stability and abundance of the Syb1 protein . - pubmed
and their adulterants were firstly investigated and compared using saccharide mapping , enzymatic ( endo-1,3 - beta - D - glucanase and pectinase ) digestion followed by polysaccharide analysis using carbohydrate gel electrophoresis analysis . - pubmed
Cisplatin caused acute ABR threshold shift at 30 and 40 kHz , but that acute hearing loss led to less age - related hearing loss at those frequencies . - pubmed
ABR P1 amplitude input - output functions were not significantly affected by the cisplatin exposure when controlling for threshold shift . - pubmed
Meta - analyses demonstrated significant weighted mean difference ( WMD ) for hsCRP ( -0.50 mg / l , 95 % CI:-0.78 ; -0.22 ) , IL-6 ( -0.48 ng / l , 95 % CI : -0.90 ; -0.06 ) , TNF - alpha ( -0.75 pg / ml , 95 % CI : -1.34 ; -0.17 ) , fibrinogen ( -0.47 g / l , 95 % CI : -0.76 ; -0.17 ) , total cholesterol ( -0.11 mmol / l , 95 % CI : -0.21 ; -0.01 ) and HDL - C ( 0.04 mmol / l , 95 % CI : 0.03 ; 0.06 ) favouring periodontal intervention . - pubmed
Starting with the public database ChEMBL organized in the chemical space of Molecular Quantum Numbers ( MQN , a series of 42 integer value descriptors of molecular structure ) , a visual survey of nearest neighbors of the alpha7 nAChR partial agonist N -( 3R )- 1 - azabicyclo [ 2.2.2 ] oct-3 - yl-4 - chlorobenzamide ( PNU-282,987 ) pointed to N -( 2 - halobenzyl )- 3 - aminoquinuclidines as possible nAChR modulators . - pubmed
Reducing elevated plasma LDL cholesterol : the central role of the LDL receptor . - pubmed
Cyclopamine modulates gamma - secretase - mediated cleavage of amyloid precursor protein by altering its subcellular trafficking and lysosomal degradation . - pubmed
Using in vitro and in vivo models , we identified cyclopamine as a novel regulator of gamma - secretase - mediated cleavage of APP . - pubmed
We demonstrate that cyclopamine decreases Abeta generation by altering APP retrograde trafficking . - pubmed
Specifically , cyclopamine treatment reduced APP - C - terminal fragment ( CTF ) delivery to the trans - Golgi network where gamma - secretase cleavage occurs . - pubmed
Instead , cyclopamine redirects APP - CTFs to the lysosome . - pubmed
These data demonstrate that cyclopamine treatment decreases gamma - secretase - mediated cleavage of APP . - pubmed
In addition , cyclopamine treatment decreases the rate of APP - CTF degradation . - pubmed
Together , our data demonstrate that cyclopamine alters APP processing and Abeta generation by inducing changes in APP subcellular trafficking and APP - CTF degradation . - pubmed
There are 2 main cotransporters in the kidney , SGLT1 and SGLT2 , which reabsorb the glucose ( 10 % and 90 % , respectively ) and return it to the blood . - pubmed
Selective pharmacological SGLT2 inhibition increases renal glucose excretion and secondarily reduces its plasma values . - pubmed
Several clinical trials have shown that dapagliflozin ( 10mg / day ) , the first SGLT2 inhibitor commercialized in Spain , produces a statistically significant reduction in HbA1c of 0.82 - 0.97 % , both in monotherapy and in combination with metformin , glimepiride , pioglitazone , or insulin . - pubmed
Phospholipase C ( PLC ) comprises a superfamily of enzymes that play a key role in a wide array of intracellular signalling pathways , including protein kinase C and intracellular calcium . - pubmed
Exploring the copper ( II )- aminotriazole complex - binding sites of human serum albumin . - pubmed
Further fluorescent tests revealed that the quenching mechanism of HSA by Cu ( II )- ATA was overall static . - pubmed
Meanwhile , the obtained binding constant and thermodynamic parameters on complex - HSA interaction showed that the types of interaction force of Cu ( II )- ATA and HSA were hydrogen bonding , van der Waals and electrostatic . - pubmed
The analysis of three - dimensional fluorescence , circular dichroism and Fourier transform infrared spectroscopy showed that Cu ( II )- ATA induced the changes in the secondary structure of HSA . - pubmed
Furthermore , amino group and attractive electrostatic interaction of Cu ( II )- ATA greatly contributed to the hydrogen bonding , van der Waals and electrostatic interaction between Cu ( II )- ATA and HSA , as confirmed by experimental data . - pubmed
In our work , a combination of surface photovoltage spectroscopy ( SPS ) , transient photovoltage ( TPV ) technique , photoelectrochemical impedance spectroscopy ( PEIS ) and transient photocurrent measurements was used to study the real role of Ni ( OH ) 2 as a cocatalyst for the enhanced PEC performance of Ni ( OH ) 2 - modified Ti - doped alpha - Fe2O3 . - pubmed
Discovery of ( ( 4 -( 5 -( Cyclopropylcarbamoyl )- 2 - methylphenylamino )- 5 - methylpyrrolo [ 1,2 - f ][ 1,2,4 ] triazine-6 - carbonyl )( propyl ) carbamoyloxy ) methyl-2 -( 4 -( phosphonooxy ) phenyl ) acetate ( BMS-751324 ) , a Clinical Prodrug of p38alpha MAP Kinase Inhibitor . - pubmed
In search for prodrugs to address the issue of pH - dependent solubility and exposure associated with 1 ( BMS-582949 ) , a previously disclosed phase II clinical p38alpha MAP kinase inhibitor , a structurally novel clinical prodrug , 2 ( BMS-751324 ) , featuring a carbamoylmethylene linked promoiety containing hydroxyphenyl acetic acid ( HPA ) derived ester and phosphate functionalities , was identified . - pubmed
In this study , we show that curcumin at low concentrations ( 1.25 - 3.12 muM ) has a strong anti - proliferative effect on TNF - alpha - induced psoriasis - like inflammation when applied in combination with light - emitting - diode devices . - pubmed
Intraoperative peak glucose value and insulin requirement have also been reported to be lower with del Nido cardioplegia , which can have prognostic significance . - pubmed
The 3 bp in - frame deletion removes 1 of the 3 adjacent isoleucine residues in transmembrane segment DIVS6 of Nav1.6 ( p. Ile1750del ) . - pubmed
The third - generation aromatase inhibitor letrozole inhibits estrogen production , and is more efficacious than the estrogen receptor inhibitor tamoxifen . - pubmed
Recent studies have demonstrated that endogenous TRPV1 agonists are generated by oxidation of omega-6 polyunsaturated fatty acids , including both linoleic acid and arachidonic acid . - pubmed
Together , these studies point to a novel pain mechanism involving the enzymatic generation of endogenous OLAM and OAAM agonists of TRPV1 . - pubmed
Furthermore , commensal bacteria contribute to intestine epithelial homeostasis through NOX1- and dual oxidase 2 - derived reactive oxygen species ( ROS ) . - pubmed
The overproduction of superoxide anion together with inactivation of superoxide dismutase are involved in the pathogenesis of Barrett ' s esophagus and its transformation to adenocarcinoma . - pubmed
In colorectal cancer , redox signaling exhibits two Janus faces : On the one hand , NOX1 up - regulation and derived hydrogen peroxide enhance Wnt / beta - catenin and Notch proliferating pathways ; on the other hand , ROS may disrupt tumor progression through different pro - apoptotic mechanisms . - pubmed
Involvement of Glucagon - Like Peptide-1 in the Regulation of Selective Excretion of Sodium or Chloride Ions by the Kidneys . - pubmed
The increase in GLP-1 level was similar to that after oral glucose administration . - pubmed
After intraperitoneal administration of 2.5 % NaCl , GLP-1 mimetic exenatide accelerated natriuresis and urinary chloride excretion . - pubmed
These findings suggest that GLP-1 participates in selective regulation of the balance of sodium and chloride ions . - pubmed
CD169 is a Siglec that may function as an adhesion molecule and a facilitator of the recognition and internalization of sialic acid decorated apoptotic bodies and exosomes derived from tumors . - pubmed
Abiraterone acetate is the oral prodrug of abiraterone , a specific CYP17 inhibitor that blocks androgen biosynthesis within the adrenal glands , testes and tumor microenvironment . - pubmed
Keywords used for the search were erectile dysfunction , phosphodiesterase type 5 inhibitors , sildenafil , vardenafil , tadalafil , avanafil , safety , side effects , and adverse events , among others . - pubmed
Stimulation of renal collecting duct principal cells with antidiuretic hormone ( arginine - vasopressin , AVP ) results in inhibition of the small GTPase RhoA and the enrichment of the water channel aquaporin-2 ( AQP2 ) in the plasma membrane . - pubmed
Rho guanine nucleotide exchange factors ( GEFs ) interact with RhoA , catalyze the exchange of GDP for GTP and thereby activate the GTPase . - pubmed
When comparing Orx vs. Orx+G groups the following significant ( P<0.05 ) changes were observed : a further increase in number of replicating cells in zonas glomerulosa and reticularis , vasculature network presence , cortical and zona reticularis volumes , ACTH and corticosterone concentrations , and lower DHEA levels . - pubmed
Comparing Orx vs. Orx+Soy resulted in elevated ( P<0.05 ) ACTH and corticosterone levels . - pubmed
However , the CP with 2 % KOH combined with 1 % Ca ( OH ) 2 displayed similar effects to those of the MP with 3 % KOH , obtaining the cumulative methane yield of 239.8 mL gVS-1 and an improved biodegradability from 56.37 % of raw WS to 66.10 % . - pubmed
Moreover , the results of thyroid function tests ( free triiodothyronine , free thyroxine , and thyroid - stimulating hormone ) remained within normal ranges both before and after ablation . - pubmed
In addition , blood viscosity and serum lipid assays suggested that XFZY capsules could decrease serum triglycerides , total cholesterol , low - density lipoprotein cholesterol and whole blood viscosity at a low shear rate . - pubmed
An antilisteriolysin O ( LLO ) monoclonal antibody ( HID5E12D7 ; IgG2b ) covalently bound to polystyrene amidine - modified latex beads was used in a slide agglutination assay . - pubmed
The latex reagent detected 0.1 ng / ml of LLO in phosphate - buffered saline plus bovine serum albumin . - pubmed
We observed no effect on intramuscular proteins involved in lipolysis ( ATGL , G0S2 , CGI-58 , HSL ) or fatty acid transport and capacity ( CD-36 and HAD , respectively ) . - pubmed
In this study , we developed a nanodisc ( ND)-functionalized bioelectronic nose ( NBN ) based on a nickel ( Ni)-decorated carboxylated polypyrrole nanoparticle ( cPPyNP)-FET that offers the detection of liquid and gaseous cadaverine ( CV ) . - pubmed
The NBN was fabricated by the oriented assembly of TAAR13c - embedded NDs ( T13NDs ) onto the transistor with Ni / cPPyNPs . - pubmed
During the last decade , ulipristal acetate ( UPA ) , a selective progesterone receptor modulator , has emerged as the most effective EC pill , and it is now recommended as first line hormonal treatment for EC in several countries . - pubmed
In the present review , we summarize and discuss the existing evidence obtained on the effect of UPA on sperm function and post - ovulatory events as potential additional mechanisms to prevent pregnancy . - pubmed
Thus , besides the described effects on ovarian function , UPA contraceptive effectiveness might also be attributed to post - ovulatory effects , depending on the moment of the female cycle in which the drug is administered . - pubmed
We observed profound prostate - specific antigen ( PSA ) and/or objective responses to immune checkpoint blockade following prior treatment with bipolar androgen therapy ( BAT ) and enzalutamide . - pubmed
Synthesis , Antibacterial and Lipoxygenase Inhibition Studies of N -( Alkyl / aralkyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfonamides . - pubmed
Moreover , N -( 3 - phenylpropyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfonamide ( 5c ) and N -( 4 - chlorobenzyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfon - amide ( 5e ) displayed decent inhibition against lipoxygenase enzyme relative to standard Baicalein . - pubmed
Hydrothermal carbonization can play an innovative role in sewage sludge ( SS ) treatment and valorization , as well as in phosphorus recovery . - pubmed
In this study , leaching tests using nitric acid were performed on hydrochar from SS and the influence of pH ( 1 - 3.5 ) , leaching time ( 30 - 240 min ) , and solid / liquid ( S / L ) ratio ( 5 - 20 wt% ) was analyzed and optimized according to the Design of Experiments method , under the Response Surface Methodology approach . - pubmed
( 2 - Aminobenzothiazole )- Methyl-1,1 - Bisphosphonic Acids : Targeting Matrix Metalloproteinase 13 Inhibition to the Bone . - pubmed
Combining both approaches , we conducted the synthesis and preliminary biological evaluation of a series of ( 2 - aminobenzothiazole )- methyl-1,1 - bisphosphonic acids active as selective inhibitors of MMP-13 via in vitro and in silico studies , which could prove useful for the treatment of bone metastases thanks to the bone - targeting capabilities granted by the bisphosphonic acid group . - pubmed
Effect of sodium - glucose cotransporter-2 inhibitors on renal handling of electrolytes . - pubmed
In this review , we aim to describe electrolytes and acid - base abnormalities due to SGLT2i as well as to elucidate the underlying mechanism . - pubmed
Changes in bone mineral composition and plasma Ca , Mg and F were determined as well as alterations in bone pyrophosphatase and the amounts of bone cyclic-3 ' , 5 '- adenosine monophosphate ( cAMP ) . - pubmed
The levels of soluble bone pyrophosphatase were not greatly influenced by changes in dietary fluoride . - pubmed
Administration of EHDP at 20 mg P / kg , however , typically decreased the levels of bone pyrophosphatase . - pubmed
At 1 mM acetate 10 % of the cell carbon came from acetate , the rest from CO2 . - pubmed
Degradation studies showed that C1 of alanine , C1 and C4 of aspartate , and C1 and C5 of glutamate were exclusively derived from CO2 , whereas C2 and C3 of alanine and aspartate , and C3 and C4 of glutamate were partially derived from acetate . - pubmed
The [ Ca2 +] i response of the three clones to BK decreased with the receptor number / cell . - pubmed
BK at 10nM and thrombin at 2units / ml both effectively increased cytosolic [ Ca2+]i . - pubmed
BK at 10nM stimulated PGE2 production three fold over basal , while thrombin only marginally elevated PGE2 levels . - pubmed
Alone , BK stimulated a small increase in 3H - thymidine incorporation into DNA . - pubmed
This peptide processing can be specifically blocked by the angiotensin-1 converting enzyme ( ACE ) inhibitor captopril , and can occur by exposing p18 to purified ACE . - pubmed
Blood pressure and sodium concentrations in CSF and serum were significantly higher in the high salt period than the low salt period ( CSF Na+ concentration : 147.7 + /- 0.4 mmol / L vs 145.3 + /- 0.5 mmol / L ; P less than 0.001 ) . - pubmed
Levels of CSF pressure and potassium or calcium concentrations were not different between the two periods . - pubmed
The levels and the changes in sodium and NA in CSF were not significantly different between the salt - sensitive ( n = 8) and the non - salt - sensitive ( n = 7 ) subjects , but the changes in plasma NA and PRA were smaller in the salt - sensitive subjects . - pubmed
Decreased 5 - HT - mediated prolactin release in major depression . - pubmed
Glycolytic oscillations were triggered after a pulse of glucose by inhibition of mitochondrial respiration with KCN , myxothiazol and antimycin A or in mutants in the bc1 complex ( ubiquinol : cytochrome c reductase ) that were largely deficient in respiratory capacity . - pubmed
Inhibition of the adenine nucleotide translocator by preincubation with bongkrekic acid also triggered a train of damped sinusoidal oscillations after glucose addition . - pubmed
Low rates of cytosolic NADH reoxidation induced by pyrazole , an inhibitor of alcohol dehydrogenase , were also associated with the disappearance of glycolytic oscillations . - pubmed
The mitochondrial NADH dehydrogenase and the glycolytic steps associated with NADH reoxidation downstream from pyruvate appear to provide another control level of glycolysis dynamics in vivo . - pubmed
Extensive phosphorylation of pp60c - src occurs at tyrosine 527 in fibroblasts and inhibits pp60c - src kinase activity . - pubmed
These data suggest that pp60c - src in fibroblasts is normally inhibited by trans - acting factors , that either directly catalyze phosphate transfer to tyrosine 527 or augment autophosphorylation at this site . - pubmed
When log - phase cells growing in glucose were heat shocked , hsp26 concentrated in nuclei and continued to concentrate in nuclei when these cells were returned to normal temperatures for recovery . - pubmed
Similarly , in cells genetically engineered to synthesize hsp26 in the presence of galactose , hsp26 did not concentrate in nuclei , with or without a heat shock . - pubmed
In wild - type cells grown in galactose or acetate and in mitochondrial mutants grown in glucose or galactose , hsp26 also failed to concentrate in nuclei with a heat shock . - pubmed
Quantitative measurement of [ 3H]acetylcholine synthesis and storage from [ 3H ] choline were negative at the early neurula stage and in 5 to 15 - day - old NF cultures , and remained low in 5 to 15 - day - old NP cultures . - pubmed
Acetylcholine production in cocultures increased linearly with time and was always much higher than in NP cultures . - pubmed
Mortality was significantly higher in group IV which also showed doubling of myocardial malondialdehyde content relative to the non - adriamycin - treated vitamin E deficient group ( III ) . - pubmed
Total sialic acid content of the sarcolemma in groups II , III and IV was 55 , 90 and 24 % of the control values in group I. - pubmed
The two major transcripts contain NGF ( 12.5 kilodaltons [ kDa ] ) at the C - terminus and differ by alternative splicing of an N - terminal exon , so that the large precursor ( 34 kDa ) had 67 amino acids upstream of an internal signal peptide and the smaller precursor ( 27 kDa ) had this signal peptide at its N - terminus . - pubmed
In these cells , a membrane - permeable cyclic AMP analog , 8 - bromo - cyclic AMP , stimulated NGF secretion . - pubmed
The last two steps in the purification of this antibiotic included reverse - phase chromatography on C18 - bonded silica gel and countercurrent chromatography on an Ito Coil Planet Centrifuge to give material of 90 % purity . - pubmed
Molecular dynamics simulation of photodissociation of carbon monoxide from hemoglobin . - pubmed
A molecular dynamics simulation of the photodissociation of carbon monoxide from the alpha subunit of hemoglobin is described . - pubmed
The effect of the protein on the rate and extent of the displacement of the iron from the porphyrin plane was assessed by comparing the results with those obtained for an isolated complex of heme with imidazole and carbon monoxide . - pubmed
The half - time for the displacement of the iron from the porphyrin plane was found to be 50 - 150 fs for both the protein and the isolated complex . - pubmed
These results support the interpretation of optical absorption studies using 250 - fs laser pulses that the iron is displaced from the porphyrin plane within 350 fs in both hemoglobin and a free heme complex in solution . - pubmed
The new peptide had an apparent molecular weightof 4000 daltons and was more acidic than ACTH as analyzed by sodium dodecyl sulfate / urea and acidic ( pH 4.5 ) polyacrylamide gel electrophoreses , respectively . - pubmed
High performance liquid chromatographic analysis of [ 35S ] methionine - labeled tryptic fragments of the peptide revealed that this peptide contained gamma MSH - ( 1 - 7 ) within its sequence . - pubmed
In the group given 0.2 mg / kg for 90 days and that given 1.0 mg / kg for 9 or 30 days , free ACh content increased slightly but significantly , and AChE activity decreased to 58 per cent . - pubmed
The amino acid composition of this protein was different from those of AA protein . - pubmed
Addition of adenosine 3 ' , 5 '- cyclic monophosphate ( cAMP)-elevating agents [ forskolin and 3 - isobutyl-1 - methylxanthine ( I / F ) ] led to large increases in uptake of 36Cl , 125I , and 82Br into oocytes expressing wild - type CFTR or delta F508 CFTR but not sham - injected oocytes . - pubmed
I / F also stimulated halide efflux from CFTR and delta F508 oocytes in the sequence Cl & gt ; Br & gt ; I. - pubmed
cAMP - induced increases in 36Cl efflux from delta F508 oocytes were approximately 20 % of those in CFTR oocytes . - pubmed
The phorbol ester , phorbol 12 - myristate 13 - acetate , also stimulated 36Cl efflux from CFTR oocytes . - pubmed
ATP uptakes into CFTR and sham oocytes were similar , and both were reduced by I / F. However , ATP uptake into I / F - treated CFTR oocytes was slightly greater ( approximately 40 % ) than into I / F - treated sham oocytes . - pubmed
Urea uptake into CFTR and sham oocytes was similar and in both cases was increased by I / F. However , the I / F - induced increase in urea uptake into CFTR oocytes was significantly greater than for sham oocytes . - pubmed
I / F stimulated formate uptake into CFTR oocytes but not into sham oocytes . - pubmed
Fluxes of 22Na , 86Rb , 35SO4 , 32PO4 , and mannitol were unaltered by expression and activation of CFTR . - pubmed
In both cell models , in the presence of cGMP and ATP , cGKII was found to mimic the effect of the catalytic subunit of cAMP - dependent protein kinase ( cAK ) on opening CFTR - Cl - channels , albeit with different kinetics ( 2 - 3 - min lag time , reduced rate of activation ) . - pubmed
By contrast , cGKI or a monomeric cGKI catalytic fragment was incapable of opening CFTR - Cl- channels and also failed to potentiate cGKII activation of the channels . - pubmed
The slow activation by cGKII could not be ascribed to counteracting protein phosphatases , since neither calyculin A , a potent inhibitor of phosphatase 1 and 2A , nor ATP gamma S ( adenosine 5 '- O -( thiotriphosphate ) ) , producing stable thiophosphorylation , was able to enhance the activation kinetics . - pubmed
Channels preactivated by cGKII closed instantaneously upon removal of ATP and kinase but reopened in the presence of ATP alone . - pubmed
Paradoxically , immunoprecipitated CFTR or CF-2 , a cloned R domain fragment of CFTR ( amino acids 645 - 835 ) could be phosphorylated to a similar extent with only minor kinetic differences by both isotypes of cGK . - pubmed
These results indicate that cGKII , in contrast to cGKI alpha , is a potential activator of chloride transport in CFTR - expressing cell types . - pubmed
In contrast , activity of the adrenal catecholamine - synthesizing enzyme tyrosine hydroxylase , but not phenylethanolamine - N - methyl transferase , was significantly elevated in CHR compared to CTL . - pubmed
Fluticasone propionate is a new , topically active glucocorticoid which shows high specificity for the glucocorticoid receptor . - pubmed
In vitro addition of AS101 at 0.1 micrograms / ml to the in vivo UVB - treated lymphocytes raised IL-2 production to approximately double the initial level , and improved the helper activity of CD4 cells . - pubmed
The insulin gene contains multiple transcriptional elements that respond to glucose . - pubmed
The beta cells in the pancreatic islets of Langerhans increase insulin gene transcription in response to increased glucose concentration . - pubmed
We have mapped sequences within the rat insulin I gene 5 '- flanking DNA ( rInsI promoter ) that direct this transcriptional response to glucose . - pubmed
A 50 - bp sequence isolated from the rInsI promoter , the Far - FLAT minienhancer , can confer glucose responsiveness to nonresponsive promoters . - pubmed
Nuclear extracts from islets grown in various glucose concentrations demonstrate a glucose - stimulated increase in a protein complex that binds the Far element and contains the transcription factors Pan-1 and Pan-2 . - pubmed
Overexpression of intact or partially deleted Pan-1 ablates the Far - directed transcriptional response to glucose . - pubmed
We conclude that the full glucose response of the insulin promoter involves the interaction of multiple sequence elements . - pubmed
A single transgene , when placed in a catalase null background , was sufficient to restore resistance to H2O2 as well as reduce susceptibility to early death . - pubmed
The Drosophila catalase gene is composed of 3 exons , encoding 19 , 307 , and 180 amino acids , which are separated by 3520- and 96 - bp introns . - pubmed
Sequence analysis of the promoter domain is presented , revealing multiple sequence similarities between catalase and Cu , Zn superoxide dismutase promoter domains . - pubmed
Developmental RNA get analysis shows that peaks of catalase mRNA accumulation correspond roughly with major peaks of ecdysone titer during third instar and pupal stages . - pubmed
Candidate ecdysone response element sequences are noted downstream of the catalase polyadenylation site . - pubmed
Addition of free radical scavengers such as , superoxide dismutase ( 100 units / ml ) , catalase ( 150 units / ml ) , mannitol ( 1 mM ) and beta - carotene ( 0.5 mM ) to the reaction system showed an insignificant effect on lipid peroxidation . - pubmed
In this system a concomitant increase in the activity of microsomal glutathione - S - transferase was observed which may serve as an alternative pathway to detoxify lipid peroxides . - pubmed
Addition of glutathione alone to the reaction system prevented both against the loss in vitamin E content and increase in the activity of glutathione - S - transferase . - pubmed
Supplementation of both vitamin E and glutathione was found to be effective in lowering glutathione - S - transferase activity to that of normal basal level . - pubmed
Our data further suggest that the lung tissue may have an inbuilt mechanism whereby glutathione - S - transferase may be triggered to cope with the excessive production of lipid peroxides . - pubmed
The only clearly identifiable cells that were [ 3H ] thymidine - positive were astrocytes , based on GFAP staining . - pubmed
The normal BBB is relatively permeable to water , but considerably less so to ions , including the principal electrolytes Brain fluid regulation takes place within the context of systemic fluid volume control , which depends on the mutual interaction of osmo- , volume- , and pressure - receptors in the hypothalamus , heart and kidney , hormones such as vasopressin , renin - angiotensin , aldosterone , atriopeptins , and digitalis - like immunoreactive substance , and their respective sites of action . - pubmed
Left ventricular wall thickness was measured by echocardiography , and relative amounts of myosin heavy chain isoforms were assessed by pyrophosphate gels . - pubmed
However , the decrease in systolic blood pressure caused by nifedipine continued for no more than 8 hours , whereas the blood pressure - lowering effect of amlodipine continued for more than 16 hours post dose . - pubmed
Amlodipine , but not nifedipine , prevented an increase in the relative amount of V3 myosin heavy chain isoform and suppressed an increase in mRNA levels of beta - myosin heavy chain , skeletal alpha - actin , and type 1 collagen . - pubmed
An assay has been developed that allows the measurement of remnant - like particle cholesterol ( RLP - C ) and triglyceride ( RLP - TG ) . - pubmed
The inhibitory effects of nucleotides are mediated via the pore - forming subunit , Kir6.2 , whereas the potentiatory effects are conferred by the sulfonylurea receptor subunit , SUR . - pubmed
The stimulatory action of Mg - nucleotides complicates analysis of nucleotide inhibition of Kir6 . - pubmed
We therefore used a truncated isoform of Kir6.2 , that expresses ATP - sensitive channels in the absence of SUR1 , to explore the mechanism of nucleotide inhibition . - pubmed
We found that Kir6.2 is highly selective for ATP , and that both the adenine moiety and the beta - phosphate contribute to specificity . - pubmed
The emission spectrum was excited in a Be hollow cathode discharge lamp with a He / D2 gas mixture . - pubmed
The osteolytic process is mainly characterized by an osteoclastic activity of bone resorption and inflammatory activity provoked by various cytokines and prostaglandins . - pubmed
Exploring structure - activity relationships around the phosphomannose isomerase inhibitor AF14049 via combinatorial synthesis . - pubmed
Solid - phase synthetic methods were developed and a series of libraries and discrete analogs synthesized to explore SAR around AF14049 . - pubmed
Thymidine dinucleotide mimics the effect of solar simulated irradiation on p53 and p53 - regulated proteins . - pubmed
We find that , like UV irradiation , pTpT increases the levels of p53 , p21 , and proliferating - cell nuclear antigen . - pubmed
The total group population with normal calcium intake ( & gt ; 1,000 mg / day ) showed higher BMD ( proximal femur and spine ) than the group with low calcium intake , this variation not being observed in group BB alleles . - pubmed
CONCLUSIONS : Our results indicate an effect of the VDR genotype on BMD proximal femur which is clearly influenced by calcium intake and calcidiol serum levels . - pubmed
Phenylarsine oxide , which inhibits the translocation of glucose transporters , decreased brazilin - stimulated glucose transport to the basal level . - pubmed
The inhibition of phosphatidylinositol 3 - kinase ( PI3 - kinase ) with wortmannin also blocked brazilin - stimulated glucose transport . - pubmed
Western blot analysis with an anti - GLUT4 antibody revealed that brazilin increased the translocation of GLUT4 from intracellular pools to the plasma membrane . - pubmed
Protein kinase C activity was not influenced by brazilin treatment . - pubmed
The inhibition of protein synthesis showed no effect on brazilin - stimulated glucose transport , and GLUT4 content in the total membrane fraction was not altered as a result of treatment with brazilin for 4 hr . - pubmed
Metabolic labeling of GLUT4 with [ 35S ] methionine showed that de novo synthesis of GLUT4 was not induced by brazilin . - pubmed
These data suggest that brazilin may increase glucose transport by recruitment of GLUT4 from intracellular pools to the plasma membrane of adipocytes via the activation of PI3 - kinase . - pubmed
However , the effect of brazilin may not be mediated by GLUT4 synthesis and protein kinase C activation . - pubmed
Genetic polymorphism in either CYP1A1 or glutathione S - transferase is linked to an increased risk of smoking related cancers , while N - acetyltransferase activity is related to cancers in which aromatic amines are the main risk factor . - pubmed
In contrast , a supramaximal dose of indomethacin ( 6 mg / kg ) partially inhibited , and dexamethasone completely prevented the carrageenan - induced increase in NGF . - pubmed
BACKGROUND : The tetra - acetate ester of 2 - deoxy - D - glucose was recently found to either inhibit or augment insulin secretion , depending on the concentration of the ester . - pubmed
The precise role of apoptosis in the pathogenesis of hepatitis C - related liver injury remains unclear , but its induction may be related to downregulation of bcl-2 expression associated with ethanol consumption . - pubmed
Efficient measurement of ( 3 ) J(N , Cgamma ) and ( 3 ) J ( C ' , Cgamma ) coupling constants of aromatic residues in ( 13 ) C , ( 15 ) N - labeled proteins . - pubmed
Light induces phosphorylation of photosystem II ( PSII ) proteins in chloroplasts by activating the protein kinase(s ) via reduction of plastoquinone and the cytochrome b ( 6 ) f complex . - pubmed
( ii ) A disulfide bond in the LHCII kinase , rather than in its substrate , may be a target component regulated by thioredoxin . - pubmed
( iii ) The target disulfide bond in inactive LHCII kinase from dark - adapted leaves is exposed and easily reduced by external thiol mediators , whereas in the activated LHCII kinase the regulatory disulfide bond is hidden . - pubmed
( iv ) Upon high - light illumination of leaves , the target disulfide bond becomes exposed and thus is made available for reduction by thioredoxin , resulting in a stable inactivation of LHCII kinase . - pubmed
Metal - binding properties of an engineered purple CuA center in azurin . - pubmed
Harder metal ions , like Co ( II ) , bind to apo - CuA - azurin only under basic conditions ( pH 9.1 - 9.2 ) . - pubmed
The results obtained from these studies indicate that two factors influence metal binding in CuA azurin : ( 1 ) the site favors metal combinations which produce an overall +3 charge , and ( 2 ) the site binds soft , thiophilic metal ions . - pubmed
The maximal specific extracellular glucose oxidase production ( 3.17 U mg-1 dry cell mass ) was achieved when the initial dissolved oxygen concentration was 6.83 mg l-1 at the impeller speed of 420 rev min-1 and at the rate of aeration of 0.25 vvm . - pubmed
Ang II was infused when subjects were in balance on a 10 - mmol Na (+ ) intake . - pubmed
rh - DNase , rh - GH , rh - MetGH , and rh - IGF - I were freeze dried with either mannitol , sucrose , trehalose , or dextran at concentrations ranging from 0 % to 100 % ( w / w ) . - pubmed
Melting of mannitol was observed through almost all of the protein concentration range examined . - pubmed
Two melting endotherms , however , were observed for mannitol at most protein / mannitol ratios , indicating the presence of protein / mannitol interactions . - pubmed
This work suggests that direct interactions occur between proteins and carbohydrates in lyophilized mixtures . - pubmed
The protein component accounted for between 0.5 and 2.0 % of the AGPs and contained between 7 and 12 % hydroxyproline . - pubmed
HSP induction correlated with enhanced survival of neonatal cardiomyocytes after sublethal heat stress in XTT testing . - pubmed
The burst - generating mechanism in the model neuron results from the interaction of a N - methyl - D - aspartate ( NMDA)-induced current and the sodium pump . - pubmed
Cytosine deaminase/5 - fluorocytosine system had significant antitumoractivities in vivo . - pubmed
In addition , the solution structure of peptide 1 that contains a phenylalanine residue ( F186 in hCD81 ) known to be critical in the binding interaction with HCV - E2 was determined using 1D and 2D 1H NMR spectroscopy . - pubmed
The CD data recorded as a function of pH and NaCl concentration are consistent with stabilization of the helical structure from electrostatic forces for both peptides . - pubmed
BACKGROUND / PURPOSE : This randomized study examined survival ( S ) and event - free survival ( EFS ) rates using high - or standard - dose cisplatin - based combination chemotherapy and surgical resection for this subset of germ cell tumors . - pubmed
Recombinant nucleoprotein productivity in nutritional feeding cultures exposed to hyperosmolarity was about 72 % higher than that obtained in batch culture at physiological osmolarity , but 31 % was a result of feeding and the rest 41 % was a result of hyperosmolarity and increasing Na (+ ) concentration . - pubmed
[ Role of sulfhydryl groups in antitrypsin in serum ] . - pubmed
The homo - oligomer III was isolated following SDS treatment of ATP synthase , the proton - turbine III+IV was obtained by blue - native electrophoresis , and complete CFO was isolated by anion exchange chromatography of NaSCN splitted ATP synthase . - pubmed
HVR-1 quasispecies modifications occur early and are correlated to initial but not sustained response in HCV - infected patients treated with pegylated- or standard - interferon and ribavirin . - pubmed
L - Arginine is a common substrate for the enzymes arginase and nitric oxide synthase ( NOS ) . - pubmed
Arginase is activated by manganese ( Mn ) , and the consumption of a Mn - deficient ( Mn- ) diet can result in low arginase activity . - pubmed
Tension produced by NOS inhibition using N ( G ) monomethyl - L - arginine ( L - NMMA ; 10(-3 ) M ) and vasorelaxation evoked by ( i ) arginase inhibition using difluoromethylornithine ( DFMO ; 10(-7 ) M ) , ( ii ) ACh ( 10(-8)-10(-4 ) M ) in the presence of DFMO , and ( iii ) sodium nitroprusside ( 10(-9)-10(-4 ) M ) were unaffected by diet . - pubmed
In vitro - measured NOS activities allowed the estimation of cardiomyocyte capacity for NO production , a value that increased from 57 % ( sea level ) to 79 nmol NO.min(-1).g heart(-1 ) ( 4,340 m ) . - pubmed
The contribution of mtNOS to total cell NO production increased from 62 % ( sea level ) to 71 % ( 4340 m ) . - pubmed
Heart mtNOS activity showed a linear relationship with hematocrit and a biphasic quadratic association with estradiol and testosterone . - pubmed
Multivariate analysis showed that exposure to high altitude linearly associates with hematocrit and heart mtNOS activity , and that testosterone - to - estradiol ratio and heart weight were not linearly associated with mtNOS activity . - pubmed
Remifentanyl led to an improvement over the local anaesthetic technique previously used , with a clear decrease in intra- and post - operative neurovegetative symptoms such as dizziness , nausea , vomiting and pain , as well as reduced bleeding . - pubmed
AhR - active compounds were isolated from sediments by Soxhlet extraction , and the crude extracts were fractionated using a Florisil column into three Fractions . - pubmed
It was determined that sediments from the Dagu River contained greater concentrations of TCDD - EQ than did sediments from the Haihe River except at Jingangqiao ( location R3 ) , which is associated with industrial activities in the adjacent densely populated area . - pubmed
With confocal microscopy , we demonstrated that calcium rise , induced by the perifusion of a solution containing a high potassium concentration , was higher in SolC1(- ) than in SolD(+ ) myotubes . - pubmed
The analysis of amplitude and kinetics of the calcium increase in SolC1(- ) and in SolD(+ ) myotubes during the exposure with SR Ca2 + channel inhibitors ( ryanodine and 2 - APB ) suggested the presence of two mechanisms of SR calcium release : ( 1 ) a fast SR calcium release that depended on ryanodine receptors and ( 2 ) a slow SR calcium release mediated by IP3 receptors . - pubmed
Furthermore , analysis of the kinetics of the rise in calcium revealed that the slow IP3 - dependent release may be increased in the SolC1(- ) as compared to the SolD(+ ) , suggesting an inhibitory effect of mini - dystrophin in this signaling pathway . - pubmed
Upon incubation with pertussis toxin ( PTX ) , an inhibitory effect similar to that of the IP3R inhibitor ( 2 - APB ) was observed on K+-evoked calcium release . - pubmed
A hypothetical model is depicted in which both Gi protein and IP3 production could be involved in K+-evoked calcium release as well as a possible interaction with mini - dystrophin . - pubmed
Our findings demonstrate the existence of a potential relationship between mini - dystrophin and SR calcium release as well as a regulatory role of mini - dystrophin on intracellular signaling . - pubmed
Furthermore , we demonstrated that application of N - propanoylmannosamine leads to a decrease in the polysialylation of the neural cell adhesion molecule in vivo , which is a marker of poor prognosis for some tumors with high metastatic potential . - pubmed
Hemoglobin Constant Spring ( Hb CS ) is an unstable hemoglobin ( Hb ) variant that results from a nucleotide substitution at the termination codon of the alpha2 - globin gene . - pubmed
Pgp and MRP activities were measured based on the extrusion of rhodamine 123 ( for Pgp ) and Fluo-3 ( for MRP ) . - pubmed
We showed previously that the synthetic peptide P(111 - 136 ) composed of the last 26 HARP amino acids inhibited HARP - induced mitogenesis . - pubmed
AgPNP has kcat values of 54 and 41 s-1 for 2 '- deoxyinosine and inosine , its preferred substrates , and 1.0 s-1 for guanosine . - pubmed
This is the tightest - binding inhibitor known for any PNP , with a remarkable Km / Ki * of 5.4 x 10(7 ) , and is consistent with enzymatic transition state predictions of enhanced transition - state analogue binding in enzymes with enhanced catalytic efficiency . - pubmed
Our data show that length - normalized Hg concentrations ( & gt ; or = 97 % as MeHg in tissue of both species ) were 230 % greater in GS in comparison to RS collected from the same general area . - pubmed
Stable C and N isotope signatures ( delta15N and delta13C ) indicate that GS occupy a slightly higher trophic position ( approximately 30 % of one trophic position higher ) on the Gulf food web in comparison to RS and that GS appear to incorporate higher trophic positioned prey , continually and at smaller sizes . - pubmed
Mercury was strongly correlated with combined delta15N and delta13C in pooled species data , arguing that most of the substantial difference in Hg bioaccumulation between RS and GS can be explained by modest differences in their trophic position and , to a lesser degree , carbon sources , which had low variation and high overlap among species . - pubmed
In this study we evaluated the apoptotic response to oxidative stress induced by 2 - deoxy - d - ribose ( dRib ) in peripheral blood lymphocytes ( PBLs ) of two siblings with Parkinson disease secondary to A53 T alpha - synuclein mutation . - pubmed
BACKGROUND : An arteriovenous loop ( AVL ) enclosed in a polycarbonate chamber in vivo , produces a fibrin exudate which acts as a provisional matrix for the development of a tissue engineered microcirculatory network . - pubmed
OBJECTIVES : By administering enoxaparin sodium - an inhibitor of fibrin polymerization , the significance of fibrin scaffold formation on AVL construct size ( including the AVL , fibrin scaffold , and new tissue growth into the fibrin ) , growth , and vascularization were assessed and compared to controls . - pubmed
Two hours before surgery and 6 hours post - surgery , saline ( Series I ) or enoxaparin sodium ( 0.6 mg / kg , Series II ) was administered intra - peritoneally . - pubmed
Reductions in the 20 - kDa myosin regulatory light chain ( MLC20 ) phosphorylation were achieved by inhibiting MLC kinase with ML-7 . - pubmed
Increases in MLC20 phosphorylation were achieved by inhibiting myosin light chain phosphatase with microcystin . - pubmed
We also found that a heptapeptide that mimics the actin - binding motif of myosin II enhanced microcystin - induced force generation and actin polymerization without affecting MLC20 phosphorylation in beta - escin - permeabilized vessels . - pubmed
Hydrogen peroxide in basic media is proposed as a means for dissolving whole blood samples to be analyzed by electrothermal atomization atomic absorption spectrometry , ET AAS . - pubmed
RESULTS : At P11 , bumetanide ( 0.5 mg / kg ) increased the baseline hippocampal afterdischarge threshold and shortened the afterdischarge duration . - pubmed
At P21 , bumetanide ( 0.2 ; 2.5 mg / kg ) exerted no effects on hippocampal excitability and kindling progression . - pubmed
DISCUSSION : The obtained results provide further evidence that bumetanide may be beneficial for treating neonatal seizures , and that NKCC1 represents a potential target for antiepileptic interventions in the immature brain . - pubmed
Expression systems based on self - cleavable intein domains allow the generation of recombinant proteins with a C - terminal thioester , providing a unique handle for site - specific conjugation using native chemical ligation ( NCL ) . - pubmed
The NMDAR contains a glycine binding site in its NR1 subunit that may be a useful target for the treatment of schizophrenia . - pubmed
The hypofunctional Dao 1(G181R ) mutation elevated brain levels of D - serine , but alone it did not affect performance in the behavioral measures . - pubmed
Diminished DAO activity and elevations in D - serine may serve as an effective therapeutic intervention for the treatment of psychiatric symptoms . - pubmed
the oxygen vacancy occurs at only the O4 site . - pubmed
Here , we sought to determine the sensitivity of the manual Polybrene test in detecting anti - Jk(a ) and anti - Jk ( b ) antibodies and Jk ( a ) and Jk ( b ) antigens . - pubmed
Furthermore , 40 randomly selected donor blood samples were collected and phenotyped for the frequencies of Jk ( a ) and Jk ( b ) antigens using the manual Polybrene test and the indirect antiglobulin test . - pubmed
RESULTS : The manual Polybrene test displayed an overall sensitivity of 60 % in detecting anti - Jk ( a ) and anti - Jk ( b ) antibody . - pubmed
Furthermore , the manual Polybrene test exhibited a sensitivity of 46.15 % for Jk ( a ) antigen and a sensitivity of 77.42 % for Jk ( b ) antigen . - pubmed
CONCLUSION : The manual Polybrene test has a very low sensitivity in detecting anti - Jk ( a ) and anti - Jk ( b ) antibody , especially anti - Jk ( a ) antibody . - pubmed
Moreover , the commercial HA ( Shanghai ) could accelerate the oxidation of different chlorophenols ( CP ) significantly under neutral condition . - pubmed
The apparent second - order rate constants of 2 - CP / phenol oxidation by permanganate in the presence of HAs correlated well with specific visible absorption ( SVA ) at 665 nm of HAs . - pubmed
70 % Ag/30 % Au was found to be optimum for SERS excited using 514.5 nm radiation , and it is explained on the basis of composition - dependent changes in the local surface plasmon resonance ( LSPR ) of the electrodeposited Ag - Au alloy . - pubmed
RESULTS : In group A ( FO ) LTB4 , TXB2 , 6 - keto prostaglandin F1alpha levels fell during omega-3 administration ( T12 ) . - pubmed
Bayesian image analysis of dexamethasone and shear stress - induced glucocorticoid receptor intracellular movement . - pubmed
Using a unique expectation - maximization ( EM ) algorithm based on Bayesian statistics , we have developed an image analysis algorithm to quantitatively assess GR nuclear translocation based on time - lapse images of green fluorescence protein - tagged GR ( GFP - GR ) under continuous exposure to a shear stress of 10 or 25 dynes / cm(2 ) as well as to Dexamethasone , a GR agonist . - pubmed
The autoregulation gene SUNN mediates changes in root organ formation in response to nitrogen through alteration of shoot - to - root auxin transport . - pubmed
RESULTS : We present an analysis of the influence of the tip apex during imaging of the Si(100 ) substrate in ultra - high vacuum ( UHV ) at 5 K using a qPlus sensor for noncontact atomic force microscopy ( NC - AFM ) . - pubmed
For bright fluorescence emissions , the carbon nanoparticles may be surface - doped with an inorganic salt and then the same organic functionalization . - pubmed
Influence of ABCC2 , SLCO1B1 , and ABCG2 polymorphisms on the pharmacokinetics of olmesartan . - pubmed
No significant differences were observed for any other single nucleotide polymorphism in ABCC2 , SLCO1B1 , or ABCG2 . - pubmed
The ABCC2 -24CC genotype group exhibited lower systemic exposure of olmesartan than the -24CT genotype group , whereas no significant differences were observed in the other transporter genotype groups . - pubmed
Collectively , these results demonstrate that CXCL14 binding to glycoproteins harboring heparan sulfate proteoglycans and sialic acids leads proliferation and migration of some cancer cells . - pubmed
Interactions of glycine betaine with proteins : insights from volume and compressibility measurements . - pubmed
Specifically , we measure the partial molar volumes and adiabatic compressibilities of cytochrome c , ribonuclease A , lysozyme , and ovalbumin in aqueous solutions of the stabilizing osmolyte glycine betaine ( GB ) at concentrations between 0 and 4 M. The fact that globular proteins do not undergo any conformational transitions in the presence of GB provides an opportunity to study the interactions of GB with proteins in their native states within the entire range of experimentally accessible GB concentrations . - pubmed
Adsorption of bovine serum albumin ( BSA ) , gamma - globulin , ( 125 ) I - BSA , pepsin , and chymotrypsin on neat and PEGylated microspheres is determined by UV - VIS spectroscopy of supernatants and eluates or by measurement of radioactivity in an ionization chamber . - pubmed
While it is thought that bioactivation to reactive Dcl acyl glucuronides ( AG ) and subsequent protein adduct formation contribute to Dcl induced liver injury , LC - MS / MS analyses did not reveal increased formation of 4 '- and 5 - hydroxy - Dcl , Dcl - AG or Dcl - AG dependent protein adducts in animals treated with LPS / Dcl . - pubmed
Hepatic gene expression analysis suggested enhanced activation of NFkappaB and MAPK pathways and up - regulation of co - stimulatory molecules ( IL-1beta , TNF - alpha , CINC-1 ) by LPS / Dcl and PIC / Dcl , while protective factors ( HSPs , SOD2 ) were down - regulated . - pubmed
Hyperbranched polyester - based fluorescent probe for histone deacetylase via aggregation - induced emission . - pubmed
Herein , we report a hyperbranched polyester - based one - step fluorescent assay for HDAC . - pubmed
HDAC triggers the deacetylation of H40 - Lys(Ac ) , thereby turning the electroneutral polymer into the positively charged one . - pubmed
Phosphorylation of Thr-457 on the short cytoplasmic domain isoform ( CEACAM1 - SF ) that is predominant in normal epithelial cells is required for lumen formation in a three - dimensional model that involves apoptosis of the central acinar cells . - pubmed
We conclude that CaMKIID specifically phosphorylates Thr-457 on CEACAM1 - SF , which in turn regulates the process of lumen formation via apoptosis of the central acinar cells . - pubmed
CONCLUSIONS : HGF - loaded PLA - O - CMC nanoparticles can steadily release HGF , and exhibits better tendencies in liver regeneration , survival rate and hepatic function compared with intravenous HGF . - pubmed
In this study , two dimensional ( 2D ) liquid chromatography ( LC ) was coupled in - line with ozonolysis - mass spectrometry ( O3 - MS ) for the elucidation of phosphatidylcholine ( PC ) structures in phospholipid extracts . - pubmed
In O3 - MS , PC molecules carried by mobile phase pass through a length of gas - permeable tubing surrounded by ozone vapor resulting in oxidative cleavage of double bonds . - pubmed
Thus , by combing the separation power of 2D - LC with in - line O3 - MS ( 2D - LC / O3 - MS ) , PC species in complex mixtures can be identified including the detailed structure of their two fatty acid chains . - pubmed
Among the 15 variables , NaNO3 , KH2PO4 and Tween 80 were found as the most significant factors with positive effect on beta - glucosidase production . - pubmed
Airway hyperresponsiveness ( AHR ) to methacholine ( Mch ) , lung histopathology analysis , inflammatory mediators in serum ( IL-4 , IL-5 , IL-17A , IFN - gamma , and eotaxin ) and in lung ( IL-4 , IL-5 , IFN - gamma , and eotaxin ) were selected for investigation and comparison . - pubmed
The effect of HD in lowering the IL-4 and IL-17A level was significantly greater than that of GD ( P & lt ; 0.05 ) , and was not significantly different from DEX ( P & gt ; 0.05 ) . - pubmed
HD treatment was more effective in down - regulation of IL-5 in serum and eotaxin level both in serum and lung than DEX ( P & lt ; 0.05 ) . - pubmed
However , the effect of HD treatment on increase of IFN - gamma mRNA expression was more apparent than GD and DEX treatment ( P & lt ; 0.05 ) . - pubmed
It has been shown the decrease of physical activity , increasing of corticosterone 's level and gene expression of cytokines after injection of Poly I : C as a model of postviral fatigue . - pubmed
In a second trial , NE was given at 100 ppm alone ( D1 ) or in combination with the prebiotic PREVIDA ( 0.5 % ) ( PRE ) ( D2 ) for 17 weeks . - pubmed
In Alzheimer ' s disease ( AD ) , neuritic plaques composed of beta - amyloid ( Abeta ) deposits and dystrophic neurites occur frequently around cerebral vasculature , raising a compelling question as to whether , and if so , how , microvascular abnormality and amyloid / neuritic pathology might be causally related . - pubmed
Analyses of electrolyte leakage revealed more extensive cell death in the control plants than in NbMADS1 - silenced plants . - pubmed
The compromised stomatal closure observed in the NbMADS1 - silenced plants was inhibited by the application of H2O2 and sodium nitroprusside ( an NO donor ) . - pubmed
Design , synthesis , and evaluation of guanylhydrazones as potential inhibitors or reactivators of acetylcholinesterase . - pubmed
Analogs of pralidoxime , which is a commercial antidote for intoxication from neurotoxic organophosphorus compounds , were designed , synthesized , characterized , and tested as potential inhibitors or reactivators of acetylcholinesterase ( AChE ) using the Ellman 's test , nuclear magnetic resonance , and molecular modeling . - pubmed
The most effective AChE inhibitor discovered was the guanylhydrazone derived from 2,4 - dinitrobenzaldehyde and was compared with tacrine , displaying similar activity to this reference material . - pubmed
Enhanced Sodium Ion Storage Behavior of P2 - Type Na ( 2 / 3 ) Fe ( 1 / 2 ) Mn ( 1 / 2 ) O2 Synthesized via a Chelating Agent Assisted Route . - pubmed
In this work , P2 - type Na2/3Fe1/2Mn1/2O2 layered materials were synthesized by a chelating agent assisted sol - gel method with NH3 H2O. - pubmed
For long - term cycling , it is preferred to cut off at 4.0 V rather than at 4.3 V. For the optimized P2 - type Na2/3Fe1/2Mn1/2O2 calcined at 900 C , a discharge capacity of 92 mAh / g remains after 40 cycles in the voltage range of 1.5 - 4.0 V , and the Coulombic efficiency remains 100 % . - pubmed
Intravesical Bacillus Calmette - Guerin Versus Combination of Epirubicin and Interferon - alpha2a in Reducing Recurrence of Non - Muscle - invasive Bladder Carcinoma : FinnBladder-6 Study . - pubmed
INTERVENTIONS : The four - arm study involved a single perioperative instillation of EPI plus five weekly instillations of BCG or EPI / IFN , followed by 11 monthly instillations in the 1 - yr BCG or EPI / IFN maintenance arms , further followed by four additional quarterly instillations in the two 2 - yr maintenance arms . - pubmed
RESULTS AND LIMITATIONS : The median follow - up time was 7.5 and 7.4 yr in the BCG and EPI / IFN groups , respectively . - pubmed
The probability of recurrence was significantly lower in the BCG group than in the EPI / IFN group . - pubmed
CONCLUSIONS : The monthly maintenance BCG regimen showed excellent efficacy and was significantly better in preventing recurrence than a similar regimen of EPI / IFN - alpha2a . - pubmed
PATIENT SUMMARY : A monthly bacillus Calmette - Guerin ( BCG ) regimen showed excellent efficacy and was significantly better in preventing recurrence than a similar regimen of epirubicin and interferon - alpha2a . - pubmed
Paclitaxel is mainly inactivated in vivo by cytochrome P5402C8 ( CYP2C8 ) . - pubmed
In recent years , the clopidogrel metabolite has been reported to potently inhibit CYP2C8 . - pubmed
MEASUREMENTS : Copeptin , serum cortisol , 24 - h urinary free cortisol , BMI - SDS and , as parameters of the MetS , insulin resistance index ( HOMA ) , HbA1c , uric acids , blood pressure and lipids . - pubmed
A 24 - h urinary free cortisol ( beta - coefficient 0 13 + - 0 06 , P & lt ; 0 001 ) , but not serum cortisol , was significantly related to copeptin in multiple linear regression analyses adjusted for age , gender , pubertal stage and BMI - SDS . - pubmed
Further studies are necessary to understand the relationship between obesity , cortisol , gender , pubertal stage and copeptin levels . - pubmed
Supt20 interacts with the Gcn5 - containing SAGA histone acetylation complex . - pubmed
In this research , beta - cyclodextrin / iron oxide reduced graphene oxide hybrid nanostructure ( beta - CD / MRGO ) with high water dispersability , excellent magnetic responsivity and molecular selectivity was prepared via a facile one step green strategy . - pubmed
Given the importance of ApoA - I in HDL and cholesterol transport , it is necessary to understand the mechanisms of ApoA - I adsorption and the associated structural changes for assessing consequences of ENM exposure . - pubmed
Here , the authors used a comprehensive array of microscopic and spectroscopic tools to elucidate the interactions between ApoA - I and 100 nm Ag nanoparticles ( AgNPs ) with four different surface functional groups . - pubmed
While the unfolding of ApoA - I on AgNPs did not significantly change their uptake and short - term cytotoxicity , the authors observed that it strongly altered the ability of only some AgNPs to generate of reactive oxygen species . - pubmed
Spasmolytic Mechanism of Aqueous Licorice Extract on Oxytocin - Induced Uterine Contraction through Inhibiting the Phosphorylation of Heat Shock Protein 27 . - pubmed
The percentage of phosphorylation of HSP27 at Ser-15 residue increased up to 50.33 % at 60 min after oxytocin stimulation . - pubmed
Colocalization between HSP27 and alpha - SMA was observed in the myometrial tissues , especially along the actin bundles in the oxytocin - stimulated group . - pubmed
Additionally , employing ChemGPS - NP provided support for a preliminary assignment of liquiritigenin and isoliquiritigenin as protein kinase C ( PKC ) inhibitors in addition to liquiritigenin , isoliquiritigenin , liquiritin and isoliquiritin as MAPK - activated protein kinase 2 ( MK2 ) inhibitors . - pubmed
Using structural magnetic resonance imaging we examined : i ) volumetric changes and white / grey matter properties of the whole cerebrum and of specific brain areas ; and ii ) whether potential alterations could be normalized by blocking glucocorticoid receptors with mifepristone during the critical developmental window of early adolescence , i.e. - pubmed
METHODS : In this study , 40 Iranian patients were investigated for the presence of the mutations in 6 mitochondrial tRNA genes ( tRNA Ile , Met , Gln , Asn , Ala and Trp ) by PCR - SSCP analysis . - pubmed
[ Detection of HLA - B * 13:01 gene by dual allele - specific real - time polymerase chain reaction in patients with trichlorethylene - induced dermatitis ] . - pubmed
Conclusion : Dual allele - specific real - time PCR can be used for detection of the HLA - B * 13:01 gene in patients with trichlorethylene - induced dermatitis . - pubmed
Clade IV SWEET proteins were localized to the tonoplast , and used hexose as a substrate . - pubmed
FDA Approval Summary : Dabrafenib and Trametinib for the Treatment of Metastatic Non - Small Cell Lung Cancers Harboring BRAF V600E Mutations . - pubmed
IMPLICATIONS FOR PRACTICE : The approvals of dabrafenib and trametinib , administered concurrently , provide a new regimen for the treatment of a rare subset of non - small cell lung cancer ( NSCLC ) and demonstrate how drugs active for treatment of BRAF - mutant tumors in one setting predict efficacy and can provide supportive evidence for approval in another setting . - pubmed
Batatasin III ( 5 ) and dendrosinen B ( 9 ) showed strong alpha - glucosidase inhibitory activity . - pubmed
Dendrosinen B ( 9 ) also exhibited appreciable pancreatic lipase inhibitory effect . - pubmed
Organic coated iron minerals ( iron supplements ) were stable against strong chelators ( calcein and transferrin ) . - pubmed
And then the effects of miR-105 were evaluated by performing trypan blue staining , transwell assay , ANNEXIN - FITC / propidium iodide ( PI ) double staining and Western blot analysis . - pubmed
Besides , inhibition of mTOR and p38MAPK pathways by using Rapamycin and VX-702 abolished the regulatory effects of Mcl-1 on EMT . - pubmed
METHODS : Analyses included adipose mtDNA levels , CI and CIV activity by immunoassay , visceral adipose tissue by computed tomography , and fasting serum glucose at week 0 and week 96 of ART . - pubmed
CONCLUSIONS : Decreased adipose mitochondrial activity correlated with changes in adiponectin and glucose homeostasis on ART . - pubmed
In addition , it was revealed that resveratrol elevated ROS concentration and expression of biomarker of cell death Bax , while inhibiting Bcl2 , an anti - apoptotic protein , and reinforcing expression of p53 . - pubmed
Moreover , resveratrol remarkably increased the expressions of HIF-1alpha and p53 in PC cells . - pubmed
Resveratrol suppressed cell survival and promoted cell death , but its effects were reversed after HIF-1alpha knockdown , suggesting that the effects of resveratrol in PC are mediated via HIF-1alpha . - pubmed
CONCLUSIONS Our findings indicate that resveratrol induces apoptosis via HIF-1alpha / ROS / p53 signaling in prostate cancer cells and may be a useful therapeutic agent against prostate cancer . - pubmed
Parathyroid hormone resistance syndromes - Inactivating PTH / PTHrP signaling disorders ( iPPSDs ) . - pubmed
Metabolic disorders caused by impairments of the Gsalpha / cAMP / PKA pathway affecting the signaling of PTH / PTHrP lead to features caused by non - responsiveness of target organs , in turn leading to manifestations similar to the deficiency of the hormone itself . - pubmed
Serum Lipoprotein Profile Is Associated With Protective Effects of Oral Contraceptive Use on Multiple Sclerosis Severity : A Cross - Sectional Study . - pubmed
A pyrene - labeled m7 GTP analogue showed up to eightfold enhanced fluorescence emission upon binding to eukaryotic translation initiation factor 4E ( eIF4E ) and over 30 - fold enhancement upon cleavage by decapping scavenger ( DcpS ) enzyme . - pubmed
Degradation of RhB into small organic moieties is detected using LC - MS technique . - pubmed
Thioacetamide increased liver weight and serum alanine aminotransferase and aspartate aminotransferase levels . - pubmed
Based on the optimum condition , when the dosage of PS was 5 g / L , the initial concentration of tetracycline hydrochloride ( TC ) , oxytetracycline hydrochloride ( OTC ) and chlortetracycline hydrochloride ( CTC ) was 250 mg / L , the removal rate of PS was 55.90 % , 45.10 % and 14.98 % higher than those of EC process after 40 min treatment . - pubmed
Herein , we adopt an alternative strategy , where the bistable ferroelectric switches ( P and P ) of Sc2CO2 ( SCO ) assist the ferromagnetic states of Cr2Ge2Te6 ( CGT ) in order to achieve non - volatile memories . - pubmed
Moreover , MXene SCO , being an aided layer in multiferroic CGT / SCO hetero - structures , also modifies the electronic properties of CGT to half metal by its polarized P state . - pubmed
The plasma concentrations of LH , FSH and testosterone were low at the beginning ; each short infusion of LH - RH resulted in a transitory increase in the level of LH and testosterone while the concentration of FSH was only marginally affected . - pubmed
The response to the individual injections of LH - RH was also modified , the peak in LH being reduced in amplitude but more prolonged while the FSH and testosterone responses were both enhanced . - pubmed
When the pulsed infusion was stopped the concentration of LH and testosterone declined rapidly while the decline in FSH levels took many days . - pubmed
Plasmic digestion of fibrinogen yielding fragment X , did not reduce the accelerating affect on erythrocyte sedimentation , indicating that the intact carboxyl terminal end of the A alpha chain is unnecessary for this phenomenon . - pubmed
Lactate dehydrogenase isoenzymes of 10 museum strains of lactobacilli were examined electrophoretically in polyacrylamide gel . - pubmed
The stimulus - secretion coupling of glucose - induced insulin release - XVII . - pubmed
The usefulness of these adaptations is illustrated by their application to the simultaneous detection of three point mutations , two in the tyrosine kinase domain of the insulin receptor and one in the insulin - responsive glucose transporter ( GLUT4 ) in a highly insulin - resistant NIDDM population . - pubmed
As an extension of previous investigations on synthesis and dopamine autoreceptor activity of bicyclic ergoline analogues the tricyclic azaergoline analogues 9a and 9b were synthesized . - pubmed
Using the SH group cross - linker phenylarsine oxide as the inducer , we show that both matrix pH and the membrane potential can modulate the process of PTP induction independently of Ca2 + . - pubmed
matrix alkalinization and membrane depolarization ) , we have explored the possibility that the Ca ( 2 +)-induced changes of the delta mu H+ may contribute to PTP induction by Ca2 + . - pubmed
Our data in mitochondria treated with Ca2 + plus N - ethylmaleimide and Ca2 + plus phosphate show that membrane depolarization is a powerful inducer of the PTP . - pubmed
Taken together , our observations indicate that the PTP can be controlled directly by the delta mu H+ both in the absence and presence of Ca2 + , and suggest that a collapse of the membrane potential may be the cause rather than the consequence of PTP induction under many experimental conditions . - pubmed
A new type of chloride channel has been identified by functional expression of phospholemman , a 72 - amino acid cardiac sarcolemmal protein with a single transmembrane domain . - pubmed
SCATyr ( Me ) AVP elicited outstanding antagonistic properties by blocking at concentration of 10(-7 ) M nearly completely the constrictory activity of AVP . - pubmed
Second , complementation analysis indicates that high - level expression of both the gltD and gltF genes is required for the induction of the Ntr enzymes under nitrogen - limiting conditions , indicating that the phenotype of the gltB insertion probably results from polarity on gltD and gltF. - pubmed
Evaluation of LH and FSH secretion during a 24 - h period was performed in a group of 15 PMW after a single oral dose of 600 or 1,000 mg of ipriflavone or placebo , and after 7 , 14 and 21 days of oral treatment with ipriflavone 600 mg and 1,000 mg / daily , administered in three divided doses . - pubmed
LH secretion was also evaluated during naloxone infusion before and after 21 days of ipriflavone , placebo or conjugated estrogen treatment ( 0.625 mg / day ; CE ) . - pubmed
LH response to NAL treatment was absent during ipriflavone and placebo such as it was observed before treatments . - pubmed
DESIGN : A prospective randomized trial was set up to compare long and short regimens of gonadotropin - releasing hormone agonist administration for ovulation induction in IVF . - pubmed
Furthermore , serum alpha - tocopherol correlated with retinol ( r = 0.53 , P less than 0.001 ) , selenium ( r = 0.45 , P less than 0.001 ) , and albumin ( r = 0.37 , P less than 0.001 ) in serum . - pubmed
Activation involves tryptic cleavage of an N - terminal pentapeptide Ala - Pro - Gly - Pro - Arg ( APGPR ) which is known as procolipase activation peptide ( CLAP ) . - pubmed
This loss of reactivity can be significantly slowed by the addition of 20 mmol / l Zinc ions ( Zn2 + ) , while ethylenediaminetetra - acetic acid ( EDTA ) and other protease inhibitors were ineffective . - pubmed
Additionally the increased blood flow may be associated with the previous findings of increased synthesis of prostaglandins , gastric mucus and bicarbonate secretion by CBS . - pubmed
The wild - type and mutant PDH complexes were purified to near - homogeneity and were analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis , immunoblotting , and enzyme assays . - pubmed
Deletion of most of the putative lipoyl domain ( residues 8 - 84 ) resulted in loss of about 85 % of the overall activity , but did not affect the acetyltransferase activity of E2 or the binding of pyruvate dehydrogenase ( E1 ) , dihydrolipoamide dehydrogenase ( E3 ) , and protein X to the truncated E2 . - pubmed
Although the lipoyl domain of E2 and/or its covalently bound lipoyl moiety were removed , the mutant complexes retained 12 - 15 % of the overall activity of the wild - type PDH complex . - pubmed
Replacement of both lysine-47 in E2 and the equivalent lysine-43 in protein X by arginine resulted in complete loss of overall activity of the mutant PDH complex. - pubmed
The cell adhesive proteins collagen and fibronectin , and the cell adhesive oligopeptide RGDS ( arginine - glycine - aspartic acid - serine ) were successfully covalently immobilized on poly ( vinyl alcohol ) ( PVA ) hydrogel sheets . - pubmed
Regulation of protein kinase C activity by gangliosides . - pubmed
The activity of protein kinase C ( Ca2 +/ phospholipid - dependent enzyme ) in the presence of phosphatidylserine and its physiological regulator , diacylglycerol , could be suppressed by a mixture of brain gangliosides . - pubmed
Inhibition was observed at all concentrations of Ca2 + between 10(-8 ) and 10(-4 ) M. Inhibition of protein kinase C activity could not be reversed by increasing the concentration of diacylglycerol or the substrate , histone . - pubmed
Our results suggest that gangliosides may regulate the responsiveness of protein kinase C to diacylglycerol . - pubmed
The properties of transcellular K + movement can be adequately described by a model which provides for active K + uptake across the basolateral membrane via an electrogenic Na / K ATPase and K+ exit from the cell across the apical membrane down an electrochemical potential gradient via K+ channels which are blocked by mucosal barium . - pubmed
Paracellular biliary access of anionic horseradish peroxidase was less than 50 % that of neutral and cationic horseradish peroxidase both in the control state and when paracellular entry was augmented with 10(-10 ) M vasopressin . - pubmed
In order to predict a haemoglobin ( Hb ) rise , in response to treatment with iron from simple erythrocyte and serological parameters , we treated 28 anaemic RA patients with oral iron during 6 weeks . - pubmed
Iron treatment is only useful in iron deficient RA patients , although active RA limits maximal Hb rise . - pubmed
Pretreatment of these cells with 500ng / ml of pertussis toxin for 24h prior to addition of 5HT reduced 5HT - induced formation of inositol phosphates . - pubmed
These data suggest that a guanine nucleotide inhibitory ( Gi ) protein couples 5HT receptor to phospholipase C and TPA modulates 5HT - stimulated hydrolysis of inositol phospholipids in cultured VSMC through activation of PKC . - pubmed
In contrast , the activities of lactate and malate dehydrogenases were unchanged , and that of acetylcholinesterase apparently increased . - pubmed
Thus satisfactory histochemical demonstration of all enzymes was achieved ( except with alkaline phosphatase , lactate and malate dehydrogenases ) following the microwave - saline pretreatment . - pubmed
For this purpose a structural analysis was employed and blood levels of gonadotrophins and testosterone were radioimmunoassayed . - pubmed
Glucosidase II is an endoplasmic - reticulum - localized enzyme that cleaves the two internally alpha-1,3 - linked glucosyl residues of the oligosaccharide Glc alpha 1 - --- 2Glc alpha 1 - --- 3Glc alpha 1 - --- 3Man5 - 9GlcNAc2 during the biosynthesis of asparagine - linked glycoproteins . - pubmed
The thyroid protein kinase C was not stimulated by Ca2 + or phosphatidylserine ( PS ) , but was stimulated by the combination of the two by 570 % . - pubmed
Diolein stimulated the kinase by increasing its sensitivity to Ca2 + . - pubmed
Other phospholipids could not substitute for PS and were ineffective in stimulating the protein kinase C in the absence of diolein . - pubmed
Quercitin , a protein kinase C inhibitor in other systems , inhibited the thyroid enzyme in a dose - related manner . - pubmed
Separation of these 32P - labelled proteins by electrophoresis and subsequent autoradiography revealed that three proteins were phosphorylated by the protein kinase C of approximate molecular weights 60,000 , 45,000 , and less than 29,000 . - pubmed
DCBT irreversibly inhibits in vitro polymerization of purified tubulin , but only after a prolonged preincubation of the protein with the drug . - pubmed
Binding of radiolabeled DCBT to tubulin similarly requires a long incubation time , with the reaction not being complete even after 6 hr at 37 degrees C. A specific interaction with tubulin is also shown by the crossresistance to DCBT of Colcemid - resistant cells with an altered beta - tubulin . - pubmed
Both strains were , however , resistant to relatively high concentrations of reagent H2O2 or H2O2 enzymatically generated by glucose and glucose oxidase , KI , or lactoperoxidase ( LPO ) alone . - pubmed
Kinetic studies using small quantities of reagent or enzymatically generated H2O2 and LPO - KI showed that the system was lethal for both fungal strains within 1 min . - pubmed
Furthermore , using the glucose - glucose oxidase - LPO - KI system , it was shown that catalase , superoxide dismutase and histidine scavengers of H2O2 , superoxide anion and singlet oxygen , respectively , prevented the killing of fungus , whereas scavengers of hydroxyl radicals such as benzoate and mannitol had no effect . - pubmed
Consequently , the xanthine ( or hypoxanthine ) and xanthine oxidase system in which the main product is superoxide anion had no toxic effect on the fungus . - pubmed
The high sensitivity of dermatophytes to killing by the H2O2 - peroxidase - halide system active in polymorphonuclear neutrophils and macrophages may account in part for fungal toxicity in vivo . - pubmed
A nonhistone protein , which migrates between oxidized histone H3 and histone H1 has been found to be increased in amount following cycloheximide treatment . - pubmed
Lys- and Glu - plasminogen caused 50 % inhibition of anti - kringle 4 binding to 125I - kringle 4 at ratios of 4 and 20 mol / mol ( competitor / kringle 4 ) , respectively . - pubmed
Amyloid beta - protein activates tachykinin receptors and inositol trisphosphate accumulation by synergy with glutamate . - pubmed
ABP weakly activated tachykinin receptors , but in the presence of N - methyl - D - aspartate and alpha - amino-3 - hydroxy-5 - methylisoxazole-4 - propionate - type glutamate receptors ABP - induced responses were greatly enhanced . - pubmed
These observations suggest that in the presence of glutamate , ABP can activate tachykinin receptors and phosphatidylinositol turnover . - pubmed
A panel of 12 neutralizing anti - LtxA monoclonal antibodies also recognized specificities within the 253 - amino acid fragment . - pubmed
Prompted by the reversal of skin hyperproliferation to normal by 13 - hydroxyoctadecadienoic acid ( 13 - HODE ) , a 15 - lipoxygenase metabolite of linoleic acid , we investigated a possible mechanism for this antiproliferative action . - pubmed
To address this we first demonstrated that 13 - HODE is incorporated into epidermal phosphatidyl 4,5 - bisphosphate ( PtdIns4,5 - P2 ) and released as 13 - HODE - containing diacylglycerol by epidermal phospholipase C. - pubmed
Secondly , we tested the possibility whether this putative 13 - HODE - containing DAG ( 13HODE - DAG ) could exert a modulatory effect on epidermal protein kinase C ( PKC ) activity which previously has been associated with skin hyperproliferation . - pubmed
Unlabeled 13HODE - DAG was generated from 13 - HODE - containing phosphatidylcholine after phospholipase C hydrolytic cleavage . - pubmed
The effects of the 13HODE - DAG were determined on : i ) total epidermal PKC activity ; ii ) diolein - activated PKC activity ; and iii ) the two identified epidermal PKC - isozymes ( PKC - beta and PKC - alpha ) . - pubmed
Our data revealed over a twofold activation of total basal PKC activity by diolein . - pubmed
In contrast , replacement of diolein ( 1,2 - dioleoylglycerol ) with 13HODE - DAG ( 1 - palmitoyl,2 - 13HODE - glycerol ) in the incubation mixture exerted no effect on total basal PKC activity . - pubmed
In an another experiment , 13HODE - DAG inhibited diolein - activated PKC activity in a dose - dependent manner . - pubmed
To determine whether the effects of 13HODE - DAG are selective , we tested its effects on DEAE - Sephacel - purified and Western blot - confirmed PKC isozymes . - pubmed
Our data revealed that 13HODE - DAG selectively inhibited the activity of PKC - beta isozyme , while exerting negligible effect on the PKC - alpha isozyme . - pubmed
This selective inhibitory effect of 13HODE - DAG on a major epidermal PKC isozyme activity suggests that 13HODE - containing DAG seemingly can modulate epidermal PKC activity , which purportedly is associated with epidermal hyperproliferation . - pubmed
DNA sequence analysis of this clone , which was designated as Oryza sativa homeobox 1 ( OSH1 ) , and a genomic clone encoding the OSH1 sequence have shown that the OSH1 gene consists of five exons and encodes a polypeptide of 361 amino acid residues . - pubmed
The effects of two months of streptozotocin - induced diabetes and aldose reductase inhibition on body weight , plasma glucose , and nerve sugars and polyols were comparable to those reported previously . - pubmed
Thus , either deficits in laser Doppler blood flow and conduction are not aldose reductase inhibitor - dependent or tolrestat has some other property which offsets the beneficial effects of aldose reductase inhibition . - pubmed
Laboratory studies revealed increased total thyroxine ( T4 ) and triiodothyronine ( T3 ) concentrations , a high - normal free T4 concentration , and a normal basal thyrotropin ( TSH ) concentration with a normal response to thyroliberin ( TRH ) . - pubmed
Immunoelectrophoresis in the presence of [ 125I ] T4 or [ 125I ] T3 localized the radiolabeled iodothyronines to a band corresponding exactly to the precipitin arc of the monoclonal IgA. - pubmed
Purified IgA ( 50 micrograms ) bound both [ 125I ] T4 ( 12.3 % ) and [ 125I ] T3 ( 2.7 % ) specifically and in a dose - dependent manner . - pubmed
Scatchard analysis of competitive - binding data utilizing [ 125I ] T4 and unlabeled T4 revealed a Kd of 2.2 x 10(-7 ) mol / L. The binding capacity for T4 was approximately 7 mumol / L. Thus , in this case of IgA - secreting myeloma , the monoclonal IgA acts as an additional thyroid hormone - binding protein in serum that interferes in the T4 and T3 RIAs . - pubmed
Despite the unusual specificity of the RNase H activity of reverse transcriptase , which cleaves the RNA c - a phosphodiester rather than the junction a - C linkage , we found no major structural differences among the RNA c - a phosphodiester rather than the junction a - C linkage , we found no major structural differences among the RNA sugar conformations -- all RNA sugars were found in the normal C3'-endo A - form conformation . - pubmed
The norepinephrine transporter ( NET ) is a site of action for tricyclic antidepressant drugs and for drugs of abuse such as amphetamine and cocaine . - pubmed
Various membrane markers including lipooligosaccharide ( LOS ) , the 16 - kDa outer membrane lipoprotein ( SmpA ) , and the C subunit of the F1F0 ATPase indicated that the lowest - density membrane fraction contained outer membranes while the high - density membrane fraction contained inner membranes and that both are essentially free of contamination by the periplasmic flagella , a major contaminant of membranes isolated by other techniques . - pubmed
Multiple alignments of these Vg sequences indicate that the variation in cleavage location has not arisen through exon shuffling , but through local modifications of the amino acid sequences . - pubmed
The so - called epidermal growth factor precursor region contains two types of motifs ( cysteine - rich Class B repeats and YWXD repeats ) which occur independently of one another in diverse proteins , and are often involved in protein - protein interactions , suggesting that they potentially are involved in dimerization of VgRs and other LDLR - family proteins . - pubmed
Pancreatic polypeptide ( PP ) is a member of a family of 36 - amino acid brain - gut peptides , including neuropeptide Y ( NPY ) and polypeptide YY ( PYY ) and acting through many subtypes of Y receptors belonging to the superfamily of the G protein - coupled receptors . - pubmed
Load - dependent mechanical efficiency of individual myosin heads in skeletal muscle fibers activated by laser flash photolysis of caged calcium in the presence of a limited amount of ATP . - pubmed
Immediately before laser flash activation , almost all of the myosin heads in the fiber are in the state , M.ADP.Pi , and can undergo only one ATP hydrolysis cycle after activation . - pubmed
( + ) - Cis-3,5 - dimethyl-2 -( 3 - pyridyl ) thiazolidin-4 - one hydrochloride ( SM-12502 ) is a newly developed drug as a platelet - activating factor receptor antagonist . - pubmed
A new RFLP characterized by SacI and SphI was found to be due to the entire gene deletion of the three exons and was associated with the decreased metabolism of SM-12502 . - pubmed
On the other hand mast cells play their role in defence of peritoneal cavity via TNF - alpha and histamine release and inhibition of fibrynolysis . - pubmed
Reply : cytochrome P450 - derived eicosanoids and the vascular wall . - pubmed
These data were confirmed by the Tdt - mediated d ' UTP - FITC nick end labeling positivity of the cells and the fragmentation of the nucleus visualized by 4 ' , 6 - diamidino-2 - phenylindole and DNA ladder techniques . - pubmed
Trio is a complex protein containing two guanine nucleotide exchange factor domains each with associated pleckstrin homology domains , a serine / threonine kinase domain , two SH3 domains , an immunoglobulin - like domain , and spectrin - like repeats . - pubmed
RhoA isoprenylation is necessary for the RhoA - Trio interaction , because mutation of the RhoA carboxyl - terminal cysteine residue blocked binding . - pubmed
METHODS : After extraction , chromatographic separation was achieved on a narrow - bore C ( 18 ) column by gradient elution with fluorometric detection ; results were confirmed by liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) . - pubmed
During chronic osmotic stimulation by giving 2 % NaCl solution for 2 and 5 days , a large number of Fos - positive neurons were observed , but the cessation of chronic osmotic stimulation by normal water drinking immediately decreased the number of Fos - positive neurons to the control level within 2 h. The number of FosB - positive neurons was increased with period of chronic osmotic stimulation , and a significant number were observed 2 - 8 h after the cessation of the stimulation . - pubmed
The diet rich in MCT significantly raised the plasma level of triacylglycerols when compared to either the starch- or cellulose - rich diet . - pubmed
Postprandial insulin concentrations were lowest for the MCT diet , and concentrations of free fatty acids were highest . - pubmed
Endogenous methylarginines regulate neuronal nitric - oxide synthase and prevent excitotoxic injury . - pubmed
While guanidino - methylated arginines ( MA ) including asymmetric dimethylarginine ( ADMA ) and N ( G )- methyl - l - arginine ( NMA ) are potent competitive inhibitors of nitric oxide synthase ( NOS ) and are released upon protein degradation , it is unknown whether their intracellular concentrations are sufficient to critically regulate neuronal NO production and secondary cellular function or injury . - pubmed
Therefore , we determine the intrinsic neuronal MA concentrations and their effects on neuronal NOS function and excitotoxic injury . - pubmed
Normal neuronal concentrations of all NOS - inhibiting MA were determined to be approximately 15 microm , while l - arginine concentration is approximately 90 microm . - pubmed
These MA levels result in & gt;50 % inhibition of NO generation from neuronal NOS . - pubmed
Thus , neuronal MA profoundly modulate NOS function and suppress NO mediated injury . - pubmed
GSNO ( 1,000 microM ) caused inhibition of collagen and ristocetin induced aggregation by approximately 50 % . - pubmed
The Coomassie blue staining intensity of all crystallin 2D gel components was 2- to 3 - fold greater in light exposed than in control retinas . - pubmed
Oxygen , beta2 agonists , and systemic corticosteroids are the mainstays of acute asthma therapy . - pubmed
FK506 inhibits Erk and PI-3K / Akt signaling , two crucial pro - survival pathways . - pubmed
The levels of phosphorylated Akt and p42 / 44 Erk decline in few hours after FK506 addition . - pubmed
Interestingly , expression of BDNF increased in a dose - dependent manner in FK506 - treated astrocytes . - pubmed
Upregulation of BDNF mRNA and protein level in astrocytes exposed to FK506 may underlie neuroprotective action of FK506 . - pubmed
Structure - function study of the amino - terminal stretch of the catalase subunit molecule in oligomerization , heme binding , and activity expression . - pubmed
CTCDelta1 - 4 and CTCDelta1 - 24 , whose N - terminal regions were shortened by four and 24 amino - acid residues , respectively , showed an 80 % decrease in specific activity compared to wild - type CTC in spite of containing the same amount of heme as in the wild - type . - pubmed
Although the smaller oligomers were found to be bound with heme , only the tetramer exhibited catalase activity in activity staining on nondenaturing gel . - pubmed
CTCDelta1 - 49 , a mutant with deletion of the N - terminal 49 amino - acid residues which contain the conserved helix alpha2 , showed no catalase activity and no heme binding . - pubmed
However , the CD spectrum profiles of CTCDelta1 - 49 , CTCDelta1 - 4 , and CTCDelta1 - 24 indicated that these mutant subunits could attain secondary conformations similar to that of wild - type CTC , regardless of their binding with heme . - pubmed
From these results , it was concluded that the N - terminal stretch of catalase is significant for complete assembly into active tetramer and that the conserved helix alpha2 , although it has little effect on the formation of the subunit secondary structure , is indispensable not only in assembling tetramer but also in binding heme . - pubmed
To regulate the production of proinflammatory compounds , a specific peptide inhibitor Val - Ala - Phe - Arg - Ser ( VAFRS ) for the group I PLA ( 2 ) enzymes has been designed and synthesized . - pubmed
Cyclin D1 mRNA and protein expressions were significantly lower in the PVL than in the PVNL and HEP at 18 h. The levels of mRNA and protein expression of cyclin A were significantly lower in the PVL than in the PVNL and HEP at 36 h. Liver regeneration , assessed by the relative liver weight , thymidine incorporation into DNA , and proliferating cell nuclear antigen ( PCNA ) labeling index was delayed significantly in the PVNL compared to that in the HEP . - pubmed
A rise in D - glucose concentration from 3.3 to 5.0 or 7.3 mM or the administration of D - fructose ( 17 and 40 mM ) in the presence of 3.3 mM D - glucose stimulated insulin release in a concentration - related manner , but failed to affect somatostatin output . - pubmed
The secretory response to L - arginine ( 5 mM ) , 25 min after restoring the basal concentration of D - glucose , was more markedly affected , in terms of potentiation of insulin and somatostatin release and reduction of glucagon output , after prior administration of D - fructose than after a prior increase in D - glucose concentration . - pubmed
A combination of selective COX-2 inhibitors and hydrogen peroxide increase the reactive oxygen species formation in osteosarcoma cells after X - ray irradiation . - pubmed
We investigated whether a combination of selective COX-2 inhibitors and hydrogen peroxide increases the effect of X - ray irradiation , with regard to reactive oxygen species ( ROS ) formation in an osteosarcoma cell line . - pubmed
A low dose concentration of COX-2 inhibitor in combination with hydrogen peroxide and irradiation did affect ROS formation in the intracellular compartment ; however , this same combination of agents at high doses did not modulate the effect of irradiation . - pubmed
Therefore , low doses of COX-2 inhibitor and hydrogen peroxide together , in combination with irradiation , is a potentially useful alternative form of radiotherapy for apoptotic - resistant neoplasms such as osteosarcoma . - pubmed
Regardless of which mechanism is correct , the selective toxicity of chloroquine for malaria parasites is probably due to permease - mediated uptake . - pubmed
Therefore we analyzed serum oxdative stress marker ( 8 - OHdG ) and anti oxidative substances ( Mn - SOD and TRX ) to evaluate their clinical significance . - pubmed
Though there was no relation between serum level of 8 - OHdG and clinical data , positive correlation between serum level of Mn - SOD , TRX and serum level of ALP , IgM was observed . - pubmed
It also made clear that genotype of GST related to the titer of AMA . - pubmed
RATIONALE : Glutamate , the major excitatory neurotransmitter in the brain mediates its effects by both ionotropic and metabotropic receptor subtypes . - pubmed
Recently , the search for selective ligands for glutamate receptor subtypes has led to the discovery of 2 - methyl-6 -( phenylethynyl ) pyridine ( MPEP ) , an antagonist specific for metabotropic glutamate receptor 5 ( mGlu5 ) . - pubmed
OBJECTIVES : We sought to assess the effects of mGlu5 receptor antagonists , MPEP and 3 -[( 2 - methyl-1,3 - thiazol-4 - yl ) ethynyl ] pyridine ( MTEP ) on cortical norepinephrine ( NE ) levels . - pubmed
CONCLUSIONS : Taken together , these data show that MPEP and MTEP , through their blockade of the mGlu5 , reduce extracellular norepinephrine , the impact of which may contribute to their anxiolytic actions . - pubmed
Sarco / endoplasmic reticulum Ca ( 2 +) ATPases ( SERCAs ) pump free Ca ( 2 + ) from the cytosol into the endoplasmic reticulum . - pubmed
However , doubly transgenic males ( HCM / GSK-3beta ) demonstrated depressed contractile function , reduced sarcoplasmic ( endo ) reticulum Ca(2+)-ATPase ( SERCA ) expression , elevated atrial natriuretic factor ( ANF ) expression , and premature death . - pubmed
Role of TGF - beta and PGE2 in T cell responses during Plasmodium yoelii infection . - pubmed
We found that during acute infection DC migrate to the spleen and secrete TGF - beta , prostaglandin E2 ( PGE2 ) and IL-10 . - pubmed
During blood - stage infection , inhibition of the activity of TGF - beta and PGE2 restores the CD8 + T cell responses generated by sporozoites , increasing protection against re - infection . - pubmed
Sphingosine-1 - phosphate ( SIP ) receptor 1 and the circulatory lipid , S1P , are required for lymphocyte egress from lymphoid organs . - pubmed
Among its various pharmacological actions , TPM has been shown to inhibit the activity of specific carbonic anhydrase enzymes in the kidney . - pubmed
The increased levels of Cu and Fe in CSF may indicate an active role of these metals in the pathogenesis of Skogholt ' s disease . - pubmed
However , no association was found between genetic factors and Pgp function using rhodamine 123 accumulation . - pubmed
The putative Mn ( II ) oxidase genes were not among the predicted targets , indicating that regulation of Mn ( II ) oxidation is controlled by other factors yet to be identified . - pubmed
Nqo1 mRNA , protein , and activity levels were determined through 96 h in SD and ACI males and females following treatment with known Nqo1 inducers oltipraz and butylated hydroxyanisole . - pubmed
The ABC transporter AtABCB14 is a malate importer and modulates stomatal response to CO2 . - pubmed
Here we show that the ABC transporter AtABCB14 modulates stomatal closure on transition to elevated CO2 . - pubmed
Stomatal closure induced by high CO2 levels was accelerated in plants lacking AtABCB14 . - pubmed
Apoplastic malate has been suggested to be one of the factors mediating the stomatal response to CO2 ( Refs 4,5 ) and indeed , exogenously applied malate induced a similar AtABCB14 - dependent response as high CO2 levels . - pubmed
In isolated epidermal strips that contained only guard cells , malate - dependent stomatal closure was faster in plants lacking the AtABCB14 and slower in AtABCB14 - overexpressing plants , than in wild - type plants , indicating that AtABCB14 catalyses the transport of malate from the apoplast into guard cells . - pubmed
We therefore suggest that AtABCB14 modulates stomatal movement by transporting malate from the apoplast into guard cells , thereby increasing their osmotic pressure . - pubmed
Positive affect was inversely related to the increase in cortisol 30 min post awakening ( p=.042 ) , and unrelated to the evening decline in cortisol levels ( p=.174 ) . - pubmed
We have examined three methods for determining the local apparent contact potential difference ( CPD ): frequency modulation KPFM ( FM - KPFM ) , amplitude modulation KPFM ( AM - KPFM ) , and frequency shift - bias spectroscopy , on a test system of 3,4,9,10 - perylene tetracarboxylic dianhydride ( PTCDA ) on NaCl , an example of an organic semiconductor on a bulk insulating substrate . - pubmed
We will also discuss the distance dependence of the CPD as measured by FM - KPFM for both the PTCDA organic deposit and the NaCl substrate . - pubmed
CONCLUSION : Unlike ADC , ( 23 ) Na MRI detected within - core ischemic lesion heterogeneity . - pubmed
The tyrosine kinase Pyk2 plays a unique role in intracellular signal transduction by linking Ca ( 2 + ) influx to tyrosine phosphorylation , but the molecular mechanism of Pyk2 activation is unknown . - pubmed
In neurons , Ca ( 2 + ) influx through NMDA - type glutamate receptors causes postsynaptic clustering and autophosphorylation of endogenous Pyk2 via Ca ( 2 +)- and calmodulin - stimulated binding to PSD-95 . - pubmed
Accordingly , Ca ( 2 + ) influx promotes oligomerization and thereby autoactivation of Pyk2 by stimulating its interaction with PSD-95 . - pubmed
Platelets were stimulated with 75 muM arachidonic acid ( AA ) , 10 muM ADP or 25 muM TRAP . - pubmed
After stimulation with each agonist , a greater proportion of large platelets bound fibrinogen , VWF , P - selectin and activated integrin alphaIIbbeta3 than small platelets both in the presence and in the absence of in vitro aspirin . - pubmed
The prepared samples were characterized by FTIR , TG , SEM , and N ( 2 ) and CO ( 2 ) adsorption . - pubmed
We incorporated simulations of the pathways that translate an increase in [ Ca](cyt ) to the activation of myosin light chain ( MLC ) kinase and cell contraction , as well as the kinetics of NO and reactive oxygen species formation and their effects on [ Ca](cyt ) and MLC phosphorylation . - pubmed
This results from stimulation of Ca ( 2 + ) uptake from the cytosol into the SR via SERCA pumps , Ca ( 2 + ) efflux into the extracellular space via plasma membrane Ca ( 2 + ) pumps , and MLC phosphatase ( MLCP ) activity . - pubmed
At concentrations above 1 nM , O ( 2 )(- ) is predicted to modulate [ Ca(cyt ) ] and MCLP activity mostly by reducing NO bioavailability . - pubmed
The DVR vasoconstriction that is induced by high concentrations of H ( 2 ) O ( 2 ) can be explained by H ( 2 ) O ( 2 )-mediated downregulation of MLCP and SERCA activity . - pubmed
We conclude that intrinsic generation of NO by the DVR wall may be sufficient to inhibit vasoconstriction by maintaining suppression of MLC phosphorylation . - pubmed
In this article , the photo - degradation of 4 - chlorophenol ( 4 - CP ) under UV irradiation was studied with focus on the photodecomposition of 4 - CP by reactive oxygen species ( ROS ) . - pubmed
4 - CP underwent much faster and more complete degradation in UV / air system than in UV / N ( 2 ) system . - pubmed
Angiotensin II upregulation of cardiomyocyte adiponectin production is nitric oxide / cyclic GMP dependent . - pubmed
A nitric oxide synthase inhibitor ( Nx - nitro - l - arginine methyl ester hydrochloride ) and an analog of cGMP antagonist ( Rp-8 - Br - CGMP - S ) blocked Ang II - mediated upregulation of adiponectin . - pubmed
CONCLUSIONS : These data suggest a mechanism whereby Ang II upregulates adiponectin in NRVMs via the angiotensin type-2 receptor / nitric oxide / cGMP/ protein kinase G signaling pathway . - pubmed
HBO was administered at a pressure of 3 atmospheres absolute for 1 h with 100 % oxygen , starting at 3 h post brain ischemia in HBO groups . - pubmed
The infarct volume ( IV ) , activation of astrocyte , and level of total glutathione and lipid peroxidation ( LP ) were assessed 24 h post - reperfusion . - pubmed
Significant reduction in IV was noted in HBO group when compared with control group . - pubmed
The Paterno - Buchi ( PB ) reaction of geraniol derivatives 1 , which contain allylic alcohol functionality and unfunctionalized double bonds , with benzophenone was investigated to see the effect of the hydroxyl group on the regioselectivity of the oxetane formation , i.e. - pubmed
The oxetane 2a is derived from the PB reaction at the allylic alcohol moiety , whereas the PB reaction at the unfunctionalized double bond produces the oxetane 3a . - pubmed
The PB reaction of the hydroxy - protected methyl ether 1b and acetate 1c gave selectively oxetanes 3b , c derived from the reaction at the more nucleophilic double bond , 2/3~ 15/85 . - pubmed
The hydroxyl - group effect was found to be small , but apparently increased the formation of 2a in the PB reaction with geraniol ( 1a ) . - pubmed
Killing of chronic lymphocytic leukemia by the combination of fludarabine and oxaliplatin is dependent on the activity of XPF endonuclease . - pubmed
We postulated that a mechanism for this activity is the incorporation of fludarabine into DNA during nucleotide excision repair ( NER ) stimulated by oxaliplatin adducts . - pubmed
CONCLUSIONS : The synergistic cell killing is caused by a mechanistic interaction that requires the initiation of XPF - dependent excision repair in response to oxaliplatin adducts , and the inhibition of that process by fludarabine incorporation into the repair patch . - pubmed
Further reduction of preparative conditioning to busulfan resulted in acceptance of donor skin implanted at 2 weeks after transplantation of naive and FasL - coated BMC , whereas third - party grafts were acutely rejected . - pubmed
MS has provided a better understanding of the absorption of beta - carotene , the most potent provitamin A carotenoid , through the use of stable isotopes of beta - carotene . - pubmed
Preparation of ( 68 ) Ga - Citrate and ( 68 ) Ga - transferrin is described , with very high yield and high radiochemical purity ( RCP ) , which is ideally suited for routine clinical studies . - pubmed
One of the major limitations of ( 67 ) Ga - Citrate SPECT is the delayed post injection waiting time of 48 hrs , in contrast to 60 min post injection waiting with ( 68 ) Ga - Citrate . - pubmed
This may be due to short half - life of ( 68 ) Ga ( 68 min ) , as it would have decayed one half - life at 60 min post - imaging time , compared to the SPECT agent ( ( 67 ) Ga ) , which would require 76 hrs to undergo one half - life . - pubmed
Similarly , uptake of ( 68 ) Ga by liver , cardiac blood pool activity is much lower than ( 67 ) Ga at 60 min post injection period , may be attributed to the faster decay of ( 68 ) Ga than ( 67 ) Ga . - pubmed
In summary , preliminary reports suggest ( 68 ) Ga - Citrate PET / CT is useful in the diagnosis of suspected bone infections with reliable sensitivity , specificity , positive predictive value , negative predictive value and overall accuracy . - pubmed
Adenylate kinase isozyme 4 ( AK4 ) belongs to a family of nucleotide monophosphate kinases involved in energy metabolism . - pubmed
Degradation of chlorophenols by a sequential combination of hydrodechlorination ( HDC ) and catalytic wet peroxide oxidation ( CWPO ) using a new magnetic Pd - Fe / gamma - Al2O3 catalyst has been studied . - pubmed
The sequential combination of HDC and CWPO allows overcoming some of the drawbacks of both treatments applied independently . - pubmed
The HDC step achieves the complete dechlorination of chlorophenols , so that the subsequent CWPO does not lead to the formation of highly toxic chlorinated by - products and reduces significantly the organic load of the effluent . - pubmed
Therefore , the effective combination of HDC and CWPO requires that the HDC step achieves the complete dechlorination of chlorophenols but no further hydrogenation is needed . - pubmed
The Pd - Fe / gamma - Al2O3 catalyst showed a high activity in both HDC and subsequent CWPO of chlorophenols being easily separated and recovered from the reaction medium due to its ferromagnetic properties . - pubmed
In spite of a moderate loss of activity , the complete dechlorination of chlorophenol and a negligible ecotoxicity of the final effluents were maintained upon successive applications of HDC + CWPO in a four - cycles test . - pubmed
Arbuscular mycorrhizal ( AM ) fungi are the most abundant plant symbiont and a major pathway of carbon sequestration in soils . - pubmed
Changes in the environmental conditions and variability of the mycorrhizosphere may alter the diurnal patterns of productivity of AM fungi , thereby modifying soil carbon sequestration , nutrient cycling and host plant success . - pubmed
We carried out a morphologic study of the endothelium with the aid of light and transmission electron microscopy and an immunohistochemical assessment to identify adenosine , inducible nitric oxide synthase ( iNOS ) , and vascular endothelial growth factor ( VEGF ) in the vein wall . - pubmed
CONCLUSION : Endothelial integrity and adenosine , iNOS , and VEGF immunoreactivities were better preserved when the no - touch technique was used for vein graft harvesting . - pubmed
Progression of hepatic fibrosis was evaluated by histopathology , hydroxyproline content , and DWI - MRI to determine the apparent diffusion coefficient ( ADC ) . - pubmed
Direct biomarkers for liver fibrosis , including transforming growth factor - beta1 , tissue inhibitor of matrix metalloproteinase-1 ( TIMP-1 ) , hyaluronic acid ( HA ) , procollagen type III amino - terminal peptide ( PIIINP ) and osteopontin ( OPN ) , were measured . - pubmed
The indirect biomarkers aspartate and alanine aminotransferases , albumin and bilirubin were also tested . - pubmed
Urological examination revealed a negative urine , bilateral large ureterohydronephrosis on USG , and serum creatinine 231 - 250 micromol / l. CT displayed the urinary bladder completely herniated into the scrotum with distal parts of both ureters and small intestine , and bilateral large ureterohydronephrosis . - pubmed
The anti - tumor effect of shikonin on osteosarcoma by inducing RIP1 and RIP3 dependent necroptosis . - pubmed
The cell death induced by shikonin could be mostly rescued by specific necroptosis inhibitor necrostatin-1 , but not by general caspase inhibitor Z - VAD - FMK . - pubmed
The protein levels of RIP1 and RIP3 in primary tumor tissues were increased by shikonin . - pubmed
CONCLUSIONS : Shikonin had prompt but profound anti - tumor effect on both primary and metastatic osteosarcoma , probably by inducing RIP1 and RIP3 dependent necroptosis . - pubmed
For seven inhibitors , we find that with correctly converged normal mode estimates of the configurational entropy , we can correctly distinguish inhibitors in agreement with experimental data for both the MMPBSA and MMGBSA methods and thus have the ability to rank the efficacy of binding of this selection of drugs to the protease ( no account is made for free energy penalties associated with protein distortion leading to the over estimation of the binding strength of the two largest inhibitors ritonavir and atazanavir ) . - pubmed
Saturation of beta 2 - microglobulin was low ( 0.34 + - 0.1 ) and decreased discretely with time ( r ( 2 ) = 0.15 , P & lt ; 0.05 ) and significantly with TMP increases ( r ( 2 ) = 0.31 , P & lt ; 0.01 ) . - pubmed
Twenty microbial isolates that precipitated macroscopic CaCO3(s ) when grown on B4 media supplemented with calcium acetate or calcium citrate were identified . - pubmed
Three anhydrous CaCO3(s ) polymorphs ( calcite , aragonite , and vaterite ) were identified by mu - XRD , and calcite and aragonite were also identified based on TEM lattice - fringe d value measurements . - pubmed
The obtained lectin preparation ( abbreviated PIFA ) is a glycoprotein with 6.5 + -1 % carbohydrates , molecular mass of 64 kDa , consisting of four identical subunits . - pubmed
Cellulose nanocrystals ( CNs ) were prepared from tunicate by enzymatic hydrolysis ( ECN ) , TEMPO - mediated oxidation ( TCN ) and acid hydrolysis ( ACN ) . - pubmed
The final PAN nanofibers were characterized by scanning electron microscopy ( SEM ) , energy dispersive spectrometer ( EDS ) , transmission electron microscopy ( TEM ) , TEM - selected area electron diffraction ( SAED ) , X - ray diffraction ( XRD ) and thermogravimetric analysis ( TGA ) . - pubmed
SEM , TEM micrographs and SAED patterns confirmed homogeneous dispersion of the silver nanoparticles which were composed of monocrystals with diameters 20 - 30 nm . - pubmed
EDS and XRD results showed that these monocrystals tended to form face - centered cubic single silver . - pubmed
Various analyses were performed on parameters including body weight , tumor weight , in situ expression of MMP-2 and VEGF , and the plasma concentrations of epinephrine , norepinephrine and glucocorticoid . - pubmed
Placental Barrier and Autism Spectrum Disorders : The Role of Prolactin and Dopamine on the Developing Fetal Brain . - pubmed
Dopamine and prolactin exhibit opposite effects on lactation . - pubmed
However , a possible role for increased prolactin / dopamine ratio in postpartum mood and thought disorders and as a prognostic indicator of the mother 's future mental health has not been well investigated . - pubmed
This review article explores a possible link between in - utero exposure to a high maternal prolactin / dopamine ratio and subsequent development of autism spectrum disorders . - pubmed
This approach was based on combining the two privileged structures , BS and THQ , which are known to be active . - pubmed
The number of GPIIb / IIIa receptors occupied by the drugs was assayed by flow cytometry using two CD61 surface fluorescein antibodies . - pubmed
Naringin showed significantly greater percentage occupation of GPIIb / IIIa receptors than did naringenin ( 14.82 + -0.81 % vs. 3.90 + -0.55 % ) , and esculetin returned significantly higher values than fraxetin and coumarin ( 12.47 + -0.97 vs. 7.53 + -0.49 and 7.90 + -0.69 respectively ) . - pubmed
Naringin was the best antiplatelet compound , showing the greatest antiplatelet activity and the highest percentage binding of GPIIb / IIIa receptors . - pubmed
Here , a potential application of the forward osmosis ( FO ) filtration process with ammonium bicarbonate ( NH4HCO3 ) as a draw solution ( DS ) was investigated to remediate membrane bioreactor - treated LL ( M - LL ) . - pubmed
In conclusion , this novel FO system using NH4HCO3 as the DS provides a cheap and efficient protocol to effectively remove PAHs and other pollutants in LL , and the diluted DS can be directly applied to crops as a liquid fertilizer , indicating that this technique is effective and eco - friendly for the treatment of different types of LL . - pubmed
Indeed , alpha - synuclein accumulation can impair synaptic dopamine release and induces the death of nigrostriatal neurons . - pubmed
Considering the known effects of TMS and the pathophysiology of traumatic brain injury ( TBI ) , TMS was proposed to have potential for treating this condition . - pubmed
The modified neurological severity score of the TMS group was lower compared with the control group at post - TBI day 14 . - pubmed
According to the results of hematoxylin eosin staining , relative cerebral parenchyma loss was lower at post - TBI day 28 in the TMS group compared with the control group . - pubmed
A significantly higher rate of neuron survival at day 28 ( P<0.05 ; indicated by NeuN ) and a significantly reduced rate of apoptosis at days 7 and 14 ( P<0.05 ; indicated by caspase-3 ) were observed in the perilesional zone of the TMS group , as compared with the control group . - pubmed
HES1 , KLF4 , STAT3 , IL-10 and SOCS3 levels were determined using western blotting and qRT - PCR , while phospho - STAT3 levels were assessed by western blotting . - pubmed
Field surveys of the impact of environmental estrogen ( EE ) pollution in aquatic wildlife have been conducted using vitellogenin ( VTG ) as a biomarker to evaluate the influence of EE . - pubmed
Mean plasma VTG and E2 levels exhibited similar changes , increasing in the yolk vesicle stage and peaking in the tertiary yolk stage in females . - pubmed
However , plasma VTG and E2 levels showed no significant changes in males , remaining at low levels throughout the reproductive cycle . - pubmed
6 - Substituted Hexamethylene Amiloride ( HMA ) Derivatives as Potent and Selective Inhibitors of the Human Urokinase Plasminogen Activator for Use in Cancer . - pubmed
This study reports the discovery of 6 - substituted HMA analogs that show nanomolar potency against uPA , high selectivity over related trypsin - like serine proteases , and minimal inhibitory effects against epithelial sodium channels ( ENaC ) , the diuretic and antikaliuretic target of amiloride . - pubmed
The results support further evaluation of 6 - substituted HMA derivatives as uPA - targeting anticancer drugs . - pubmed
UHPLC / MS / MS was used for the analysis of IVM faecal concentration . - pubmed
High CHOL content is found to exert strong inhibitory effects on Kv channels . - pubmed
Studying anemia of chronic disease and iron deficiency in patients with rheumatoid arthritis by iron status and circulating hepcidin . - pubmed
Functional iron deficiency in ACD can be attributed to overexpression of the main iron regulatory hormone hepcidin leading to diversion of iron from the circulation into storage sites resulting in iron - restricted erythropoiesis . - pubmed
Complete blood counts , serum iron , total iron binding capacity , ferritin , and hepcidin-25 were assessed . - pubmed
It was strongly and positively correlated with ferritin ( P<0.001 ) , while hemoglobin , serum iron , and total iron binding capacity were negatively correlated with hepcidin ( P=0.016 , 0.022 and & lt;0.001 , respectively ) . - pubmed
ATP hydrolysis activity catalyzed by chloroplast and proteobacterial ATP synthase is inhibited by their epsilon subunits . - pubmed
Moreover , systematic post - characterizations further reveal that the optimal Ni - FeS2 - 0.5 sample completely converts to amorphous Ni - doped FeOOH via an in situ electrochemical transformation with yolk - shell structure well - preserved under the OER conditions . - pubmed
Mechanisms of Blockade of the Muscle - Type Nicotinic Receptor by Benzocaine , a Permanently Uncharged Local Anesthetic . - pubmed
Many of them are protonated at physiological pH , but benzocaine ( Bzc ) is permanently uncharged , which is relevant because the effects of LAs on nicotinic acetylcholine ( ACh ) receptors ( nAChRs ) depend on their presence as uncharged or protonated species . - pubmed
Co - application of ACh with increasing concentrations of Bzc showed that Bzc reversibly blocked nAChRs . - pubmed
Besides , ACh and Bzc co - application enhanced nAChR desensitization . - pubmed
When Bzc was just pre - applied it also inhibited IACh , by blocking closed ( resting ) nAChRs . - pubmed
The electrophysiological results indicate that Bzc effects on nAChRs are similar to those of 2,6 - dimethylaniline , an analogue of the hydrophobic moiety of lidocaine . - pubmed
Furthermore , docking assays on models of the nAChR revealed that Bzc and DMA binding sites on nAChRs overlap fairly well . - pubmed
These results demonstrate that Bzc inhibits nAChRs by multiple mechanisms and contribute to better understanding both the modulation of nAChRs and how LAs elicit some of their clinical side effects . - pubmed
In this work , three chemically distinct treatments used to form structurally and mechanically stable scaffolds - EDC - NHS , genipin and tissue transglutaminase - are investigated for their effect on collagen piezolectricity . - pubmed
Crosslinking with EDC - NHS is noted to produce a distinct self - assembly of the fibres into bundles roughly 300 nm in width regardless of the collagen origin . - pubmed
Effects of palm oil consumption on biomarkers of glucose metabolism : A systematic review . - pubmed
Dietary fatty acids has also been linked to affect glucose metabolism and insulin sensitivity . - pubmed
This systematic review is aimed at critically evaluating the available evidence on the association of palm oil with the biomarkers of glucose metabolism as compared to other vegetable oils . - pubmed
Insignificant effects were also seen on fasting insulin when compared to partially hydrogenated soybean oil [ 1.72pmol / L ( -11.39,14.84 ) P = 0.80 , I2 = 12 % ] and olive oil diet [ -0.14pmol / L ( -4.87,4.59 ) P = 0.95 , I2 = 0 % ] . - pubmed
CONCLUSION : Current evidence on the effects of palm oil consumption on biomarkers of glucose metabolism is poor and limited to only healthy participants . - pubmed
We conclude that little or no additional benefit will be obtained by replacing palm oil with other oils rich in mono or polyunsaturated fatty acids for changes in glucose metabolism . - pubmed
Tagging of TurboID , an improved promiscuous biotin ligase that biotinylates neighboring proteins , to the C terminus of caspases revealed the differences among the neighbors of executioner caspases . - pubmed
Surprisingly , energy - related nutrients such as amino acids / peptides and tricarboxylic acid cycle - related metabolites were found at higher levels in SI pollinations than in SC pollinations . - pubmed
PURPOSE OF REVIEW : This article aims to give a state - of - the - art assessment of treatment options for bloodstream infection because of ceftriaxone - resistant Gram - negative bacilli , especially those caused by extended - spectrum beta - lactamase ( ESBL ) or AmpC - producing Enterobacteriaceae . - pubmed
Therefore , the mechanisms for induction of MtDNAcn and MN by benzene may be similar from exposure to low doses but different from high doses . - pubmed
Similar increase of MN frequencies and MtDNAcn may be due to oxidative stress induced by benzene at low concentrations , while higher concentrations may start to initiate the cell death pathway . - pubmed
Meanwhile , TSA substantiated adequate power to demonstrate the relationship between SOX2 - OT expression and OS . - pubmed
MgSO4 intervention in women at imminent risk for preterm birth decreased the offspring 's CP risk ( meta - analysis RR 0.68 , 95 % CI 0.54 - 0.85 ; TSA RR 0.69 , 95 % CI 0.48 - 0.97 ) . - pubmed
In addition , increasing in vivo studies further indicated that migraine - related changes such as CGRP increase , inflammation , nitric oxide increase , and spontaneous behavior problems can be reduced by UR extraction and its active constituents . - pubmed
Biohybrid colloids were fabricated based on electrostatic complexation between anionic TEMPO - oxidized cellulose nanofibrils ( TO - CNF ) and cationic hen egg white lysozyme ( HEWL ) . - pubmed
Using an Ordinal Approach to Compare Outcomes Between Vancomycin Versus Ceftaroline or Daptomycin in MRSA Bloodstream Infection . - pubmed
Tetrahydroisoquinolines derived from catecholamines or DOPA : effects on brain tyrosine hydroxylase activity . - pubmed
The enzyme molecule is a tetramer of the alpha 2 beta 2 type . - pubmed
Transketolase contains about two moles of TPP per mole of protein and does not require metal ions for its catalytic activity . - pubmed
An 11 - year - old Jewish girl of Turkish extraction with abetalipoproteinaemia was found to be homozygous for haemoglobin Szuhu ( beta80 Asn leads to Lys ) . - pubmed
[ Successful use of the erbium - Yag laser in an IVF program ] . - pubmed
mansoni antigens in particular are implicated in the immune - dependent action of PZQ : a 200 kDa glycoprotein and a 27 kDa antigen with non - specific esterase activity . - pubmed
A kit formulation for preparation of iodine-123 - IBZM : a new CNS D-2 dopamine receptor imaging agent . - pubmed
The nature of these replacements in this HLA - B35 variant is likely to affect peptide binding and recognition by T lymphocytes . - pubmed
There were significant reductions ( p less than 0.03 ) in percent recovery ( 37 degrees C and 24 degrees C ) of cardiac output , stroke work , and adenosine triphosphate level in the groups that contained 0 or 15 mmol / L Mg2 + as the calcium concentration was increased from 0.05 to 4.5 mmol / L.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
Effect of amygdala kindling on GABA neurons : histochemical and biochemical detection of GABA - T activity . - pubmed
The yield of purified enzyme was 41 % and the specific activity was 592 o - nitrophenyl beta - D - galactopyranoside U / mg at 37 degrees C. Two isozymes were present , but only one subunit was detected , having a mol . - pubmed
At the electron microscopic level , PRL - containing cells were visualized using the immunogold procedure . - pubmed
The two binding curves were not completely parallel , suggesting the existence of slight variations of the amino acid sequences of PRL in the two species . - pubmed
1000 - fold by sequential ( NH4 ) 2SO4 fractionation , gel filtration , ion - exchange and affinity chromatography on calmodulin - Sepharose 4B. - pubmed
The purified protein kinase is completely dependent on Ca2 + and calmodulin , and phosphorylates a 20 kDa light - chain subunit of intact gizzard myosin , suggesting that it belongs to a class of enzymes known as myosin light - chain kinase ( MLCK ) . - pubmed
The apparent Km values of the putative pancreatic MLCK for ATP ( 73 microM ) , gizzard myosin light chains ( 18 microM ) and calmodulin ( 2 nM ) are similar to those reported for MLCKs isolated from smooth muscle , platelet and other sources . - pubmed
After separation from unbound ligand , cell - bound IFN was extracted with the detergent digitonin yielding soluble and insoluble complexes of IFN and receptor , together with a certain amount of uncomplexed IFN . - pubmed
The total ( soluble and insoluble ) of complexed IFN obtained after digitonin extraction was a constant fraction ( 0.62 ) of the total cell - bound radioactivity , being independent of the concentration of IFN added to the cells ( less than pM to greater than nM ) , and of the time of incubation ( 1 min to 20 h ) . - pubmed
Ambulatory activity ( counts ) in the control periods was 298.2 + /- 20.1 in the dark , 35.6 + /- 9.4 in the light , and 343.9 + /- 24.1 over 24 h. After administration of STZ ( 60 mg / kg , ip ) , the ambulatory activity gradually decreased [ at 3 days : 165.0 + /- 12.3 in the dark ( P less than 0.001 ) , 34.0 + /- 10.5 in the light ( P = NS ) , and 199.0 + /- 10.5 over 24 h ( P less than 0.001 ) . - pubmed
To prevent late thrombosis of saphenous vein aortocoronary grafts , it appears reasonable that decreasing the ratios of total cholesterol / HDL cholesterol and of LDL cholesterol / HDL cholesterol , refraining from smoking , and controlling other risk factors for atherosclerosis should be advised . - pubmed
We have conducted phase I and II trials of topical tretinoin ( retinoic acid and Retin - A ) delivered by means of a cervical cap and inert collagen sponge system . - pubmed
The activity of the tagged gene as well as of the resident gene was significantly inhibited by treatment of the transgenic plants with the herbicide norfluorazon , indicating that this gene activity is dependent on the presence of functional chloroplasts in the leaves . - pubmed
Characterization of septic shock serum PLA2 revealed a calcium - dependent enzyme with absolute 2 - acyl specificity with a pH optimum of 7.5 . - pubmed
Chloroquine , chlorpromazine , dexamethasone base , dexamethasone sodium phosphate , indomethacin , lidocaine , oleic acid , palmitic acid , promethazine , trans - retinoic acid , rutin and dl - alpha - tocopherol were all studied over the range of 10(-2 ) to 10(-7 ) M. All agents , with the sole exception of dexamethasone base , inhibited PLA2 activity at concentrations greater than 10(-3 ) M. PLA2 inhibition by dexamethasone sodium phosphate was factitious , due to the formation of calcium - phosphate complexes . - pubmed
Since serum PLA2 levels correlate with mortality in both experimental endotoxemia and clinical gram - negative septic shock , and chlorpromazine was previously shown to improve survival in these conditions , we postulate that its therapeutic efficacy resides at least in part in its PLA2 - inhibitory activity . - pubmed
The HSS reference patient improved after oxygen therapy , diuretics , antibiotics , and relief of OSA . - pubmed
Damage effect of chronic intoxication by CCl 4 on structural organization of liver microsomes and cytochromes ( b ) 5 and P-450 . - pubmed
The purified enzyme catalyzes the oxidation of methanol and formaldehyde to formate ; other primary alcohols are oxidized to their corresponding aldehydes . - pubmed
Ammonium ions are required for enzyme activity . - pubmed
4 The ineffectiveness of atenolol compared to the other beta - adrenoreceptor blocking drugs may be related to the finding that , in acute experiments , atenolol inhibited tissue uptake of 3H - NA . - pubmed
Control of opiate receptor - adenylate cyclase interactions by calcium ions and guanosine-5 '- triphosphate . - pubmed
Activation of either opiate receptors by 10 microM morphine or alpha - adrenergic receptors by 10 microM norepinephrine inhibits adenylate cyclase by 55 % in the absence of Ca2 + ions , and inhibits the Ca2 + -dependent activation of adenylate cyclase by more than 90 % . - pubmed
Concentrations of Ca2 + ions greater than 0.1 mM inhibit adenylate cyclase and also reduce the extent inhibition of adenylate cyclase by morphine but not by norepinephrine . - pubmed
Guanosine-5 '- triphosphate ( 0.1 - 1 microM ) is required for inhibition of adenylate cyclase by morphine . - pubmed
The results show that morphine inhibits adenylate cyclase by a guanosine-5 - triphosphate - dependent process and that the extent of inhibition of adenylate cyclase by morphine or norepinephrine is a function of the Ca2 + ion concentration and the proportion of adenylate cyclase molecules that are activated or inhibited by Ca2 + ions . - pubmed
It was almost 50 % higher than phosphatidylcholine in the unsaturated fat group and coincided with the accumulation of an apolipoprotein E - rich high density lipoprotein . - pubmed
In a fatal case of acute parathion ingestion , paranitrophenyl sulphate , the paranitrophenyl glucuronide and free paranitrophenol excretion on day 3 were quantitated using a HPLC reverse method with a C18 column following sample preparation using a C18 mini - column and methanol elution . - pubmed
Effects of tibolone on lipoprotein ( a ) and HDL subfractions . - pubmed
OBJECTIVE : To determine the effects of tibolone , a synthetic steroid used to alleviate climacteric symptoms and prevent osteoporosis , on lipoprotein metabolism , with particular reference to lipoprotein ( a ) levels and HDL subfraction profiles . - pubmed
There were significant decreases in triglycerides and VLDL cholesterol and no significant change in LDL cholesterol . - pubmed
There was a significant reduction of 18 % in HDL cholesterol and a 26 % reduction in the HDL2 - HDL3 ratio . - pubmed
CONCLUSION : The reduction in lipoprotein ( a ) levels may have a beneficial effect on cardiovascular risk , which could go some way towards balancing the potentially adverse effect on the cardiovascular system caused by the reduction in HDL cholesterol . - pubmed
Vasotocin treatment increased adenylate cyclase activity in glomeruli and in collecting ducts and did not modify it in proximal convoluted tubules and in early and late distal tubules . - pubmed
In glomeruli , the hormonal stimulation resulted mainly in a decrease in the Km value for adenylate cyclase , which means a higher affinity for substrate ( ATP ) to the enzyme , whereas the response to forskolin was accounted for by increases both in affinity for substrate and in maximal adenylate cyclase velocity . - pubmed
This receptor apparently is coupled to a G - protein which activates cGMP - phosphodiesterase to modulate cGMP levels and thus a cGMP - gated cation channel . - pubmed
Thus , across a wide range of species , G ( o ) is present in retinal bipolar cells , and at least some of these are depolarizing and APB - sensitive . - pubmed
Changes in plasma pentosidine levels after transplantation were compared to changes in simultaneously obtained glycohemoglobin levels . - pubmed
Kidney - pancreas transplantation resulted in normal glycohemoglobin levels within three months but offered no advantage over kidney transplantation alone in the partial correction of plasma pentosidine levels . - pubmed
The unifactorial analysis ( UFA ) suggested that the following factors were associated to an statistically significant increase in the mortality due to sepsis : age above 70 ; male sex ; presence of shock upon hospitalization ; nosocomial etiology ; high levels of urea , creatinine and LDH ; proteinuria ; family of the causal germ ( gram - positive coccus ) and underlying pathology . - pubmed
The relative risks ( RR ) or " odd ratios " of creatinine and LDH were , respectively , 2.8 and 2.9 in the UFA ; 3 and 3.3 in the multivariant analysis ( MVA ) . - pubmed
The results of the univariant and multivariant analysis showing the relative risk ( odd ratio ) associated to each of these variables with statistical significance , demonstrate that the mortality rate due to sepsis increases with age , nosocomial etiology and elevation of creatinine and lactodehydrogenase ( LDH ) levels . - pubmed
Urapidil , an antihypertensive agent with dual action ( alpha 1 - adrenergic antagonist and 5HT1A agonist ) is well established in the treatment of arterial hypertension . - pubmed
These two tissues were representative of both isoforms , SI- or SI+ , and displayed the same [ 3H]InsP3 - binding characteristics . - pubmed
Deletion of the peptide 316 - 352 containing the SI segment greatly reduced InsP3 binding [ Miyawaki , Furuichi , Ryou , Yoshikawa , Nakagawa , Saitoh and Mikoshiba ( 1991 ) Proc . - pubmed
The antibodies against the SI region or against residues 337 - 349 did not modify the binding of [ 3H ] InsP3 in the cortical membranes rich in the SI+ isoform or in cerebellar membranes . - pubmed
We suggest that the domain with the SI region may be involved in a function other than InsP3 - induced Ca2 + release . - pubmed
The purpose of the exhibition was to inform members of the progress on patient education , guidance of diet and exercise treatment , the compliance situation on self - monitoring of blood glucose , self - injection of insulin , etc . - pubmed
Using colorimetric assays , we were able to show aryl sulphatase B activity ( 18.5 + /- 3.7 nM nitrocatechol/10(6 ) cells ) and detected peroxidase ( 1.86 + /- 0.03 ng/10(6 ) cells ) in EGC homogenates . - pubmed
Bilateral testicular function was determined 60 days after experimental torsion by measuring testis weights , daily sperm production ( DSP ) , and testicular venous testosterone concentrations . - pubmed
RESULTS : SOD + catalase and SOD + catalase+verapamil treatments caused significant rescue of tests function following 1 hr . - pubmed
Effects of N - methyl - D - aspartate - receptor blockade on high - potassium - induced neuronal death and glutamate release . - pubmed
In this study we determined the effects of the competitive N - methyl - D - aspartate ( NMDA )- receptor antagonist CGP 40116 . - pubmed
The aim of the present investigation was to examine if new cementum ( NC ) formed during cyclosporin A ( CsA ) administration was maintained after suspension of the treatment . - pubmed
These results suggest that ( a ) NC deposition and its functional relations with the adjacent connective tissue are not reversible after cessation of CsA treatment and ( b ) in the presence of cervical NC spurs the amount of connective tissue attachment on the root surfaces is increased . - pubmed
To block P - gp function , cyclosporin A was administered . - pubmed
This indicates complete inhibition of P - gp mediated [ 11C ] verapamil efflux . - pubmed
We conclude that cyclosporin A can fully block the P - gp function in the blood brain barrier and the testes and that PET enables the in vivo measurement of P - gp function and reversal of its function non - invasively . - pubmed
IL-13 dose - dependently ( 1 - 100 pmol / L ) reduced the incorporation rate of [ 3H ] thymidine in hOB cells by more than 50 % . - pubmed
Blood concentrations of vasopressin , neuropeptide FF and prolactin are increased by high - dose right unilateral ECT . - pubmed
The maximal elevations in circulating vasopressin and prolactin concentrations were 680 % and 950 % , respectively . - pubmed
The neuropeptide FF concentration returned to baseline level at 10 min and the vasopressin concentration at 25 min after ECT . - pubmed
In fact , these three novel metal - thiosemicarbazone compounds kill Pam 212 - ras cells through apoptosis induction . - pubmed
Crystallographic analysis of triclosan bound to enoyl reductase . - pubmed
mazei Go1 comprises 12 genes that are referred to as fpoA , B , C , D , H , I , J , K , L , M , N , and O. Analysis of the deduced amino acid sequences revealed that the enzyme is closely related to proton translocating NADH dehydrogenases of respiratory chains from bacteria ( NDH-1 ) and eukarya ( complex I ) . - pubmed
Like the NADH - dependent enzymes , the F ( 420 ) H ( 2 ) dehydrogenase is composed of three subcomplexes . - pubmed
Characterization of SB-269970 - A , a selective 5 - HT ( 7 ) receptor antagonist . - pubmed
and the non - selective 5 - HT ( 7 ) receptor antagonist metergoline ( 0.3 - 3 mg kg(-1 ) s.c . ) - pubmed
, suggesting a role for 5 - HT ( 7 ) receptor stimulation in 5 - CT induced hypothermia in guinea - pigs . - pubmed
Liver histopathology , hydroxyproline content , prolyl hydroxylase activity , collagen I and III mRNA expression and the evolution of transformed Ito cells into myofibroblasts were assessed . - pubmed
2 - Phosphonomethyl pentanedioic acid ( 2 - PMPA ) is a potent and selective inhibitor of glutamate carboxypeptidase II ( NAALADase ) , and has shown robust neuroprotective activity in both in vitro and in vivo models of ischemia . - pubmed
In the brain , glutamate carboxypeptidase II ( GCPII ) ( EC3.4.17.21 ) hydrolyzes the neuropeptide N - acetylaspartylglutamate ( NAAG ) to glutamate and N - acetylaspartate . - pubmed
Binding exhibited similar pharmacological properties to GCPII enzyme activity , including chloride dependency , cobalt stimulation and inhibition by phosphate and quisqualate . - pubmed
The binding of [ ( 3 ) H ] 2 - PMPA also showed tissue specificity in that tissues previously reported to be devoid of GCPII enzymatic activity were devoid of [ ( 3 ) H ] 2 - PMPA binding . - pubmed
[ ( 3 ) H ] 2 - PMPA binding represents an additional probe for the study of GCPII activity , and may be useful as a high throughput screening assay . - pubmed
BACKGROUND : Selective deamidation of glutamine residues by tissue transglutaminase ( tTG ) turns gliadin peptides into stronger activators of T cells from celiac disease ( CD ) patients . - pubmed
The reviewed literature includes the effects of changing the conditions , intermediates and kinetic traps , mutations , similar topologies , fragment complementation experiments , fragments and pathways , focusing on one specific well - studied example , that of the dihydrofolate reductase , chaperones , and chaperonines , in vivo vs. in vitro folding , still using the dihydrofolate example , amyloid formation , and molecular " disorder " . - pubmed
A phase I - II study of docetaxel - ifosfamide - cisplatin ( DIP ) combination chemotherapy regimen in advanced nonsmall cell lung cancer . - pubmed
G - CSF ( lenograstin ) was administered from days 4 - 13 . - pubmed
The dose - limiting toxicity ( DLT ) was reached at DL4 ( Docetaxel : 100 mg / m2 - Ifosfamide : 5 g / m2 - Cisplatin : 100 mg / m2 ) consisting of 2 cases of febrile neutropenia ( FN ) , and DL3 ( Docetaxel : 100 mg / m2 - Ifosfamide : 5 g / m2 - Cisplatin : 80 mg / m2 ) was considered as the maximum tolerated dose ( MTD ) and recommended for further phase II testing . - pubmed
In the present phase I - II study , DIP appears highly active and tolerable in advanced NSCLC in the outpatient setting . - pubmed
The telomerase - transduced cells recovered a chondrocyte - specific gene expression pattern following culture on polyHEMA - coated dishes . - pubmed
The present study examined a possible molecular mechanism involved in cAMP elevators - induced inhibition of IL-12 p40 expression in response to LPS . - pubmed
Subsequent studies revealed that cAMP - elevators blocked phosphorylation of p38 MAPK , but did not affect the activity of NF - kappaB binding to IL-12 promoter ( -136/-112 ) . - pubmed
This is the first report that cAMP elevators inhibit LPS - induced IL-12 production by a mechanism that is associated , at least in part , with p38 - dependent inhibition by cAMP signaling pathways . - pubmed
The higher the vWF levels or the lower the NO levels , the more severe in PIH . - pubmed
There was a negative correlation between the plasma vWF and NO levels . - pubmed
CONCLUSION : The results suggest that plasma vWF and NO concentration can be used as indicators in the judgement of the severity of PIH . - pubmed
Carbohydrate structural units in glycoproteins and polysaccharides as important ligands for Gal and GalNAc reactive lectins . - pubmed
Glycoproteins ( gps ) contain many carbohydrate epitopes or crypto - glycotopes for Gal and GalNAc reactive lectins . - pubmed
They are : F , GalNAcalpha1 - -& gt;3GalNAc ; A , GalNAcalpha1 - -& gt;3Gal ; T , Galbeta1 - -& gt;3GalNAc ; I , Galbeta1 - -& gt;3GlcNAc ; II , Galbeta1 - -& gt ; 4GlcNAc ; B , Galalpha1 - ->3Gal ; E , Galalpha1 - ->4Gal ; L , Galbeta1 - -> ; 4Glc ; P , GalNAcbeta1 - -& gt;3Gal ; S , GalNAcbeta1 - -& gt;4Gal and TN , GalNAcalpha1 - -& gt;Ser(Thr ) . - pubmed
TN , which is an important marker for breast / colon cancer and vaccine development , exists only in O - glycans . - pubmed
To facilitate the selection of lectins that could serve as structural probes , the carbohydrate binding properties of Gal / GalNAc reactive lectins have been classified according to their highest affinity for structural units and their binding profiles are expressed in decreasing order of reactivity . - pubmed
Hence , the binding relationship between glycoproteins and Gal / GalNAc specific lectins can be explored . - pubmed
Endothelial and subendothelial cells expressed none to scant , very weak progesterone and estrogen receptor positivity . - pubmed
Thus , increased expression of hCTR1 had a substantially larger effect on the cellular pharmacology of copper than cisplatin . - pubmed
Thus , although increased expression of hCTR1 mediates greater cellular accumulation of copper and cisplatin , hCTR1 delivers these compounds into intracellular compartments from which they do not have ready access to their key cytotoxic targets . - pubmed
Facial axotomy led to a gradual increase of B7.2 immunoreactivity ( IR ) on microglial cell surface ; similar effects were also observed following application of LPS , but both effects were not additive , suggesting overlapping or saturated signaling pathways . - pubmed
The adsorption of fibrinogen ( Fb ) and bovine serum albumin onto polycrystalline Au coated with HS ( CH2 ) 3O ( CH2CH2O ) 5CH3 was determined by surface plasmon resonance from bare Au ( 0 % coverage ) to the complete ( approximately 100 % coverage ) self - assembled monolayer ( SAM ) . - pubmed
On the basis of our data , we propose a mechanism of protein rejection by oligo ( ethylene oxide ) ( OEO)-modified surfaces in terms of changes in free energy ( DeltaGsystem ; system = protein + surface ) due to oligomer conformational constriction over an area greater than the contact area . - pubmed
Differential DNA recognition and cleavage by EcoRI dependent on the dynamic equilibrium between the two forms of the malondialdehyde - deoxyguanosine adduct . - pubmed
Hypoglycaemia induced by insulin injection is a powerful stimulus to the hypothalamic - pituitary - adrenal ( HPA ) axis and drives the secretion of corticotropin - releasing hormone and vasopressin from the neurones in the paraventricular nucleus ( PVN ) , as well as the downstream hormones , adrenocorticotropic hormone and corticosterone . - pubmed
Regulation of glutamatergic mechanisms could be involved in the control of the HPA axis during hypoglycaemic stress and one potential site of regulation might be at the receptors for glutamate , which are expressed in the PVN . - pubmed
Injection of insulin induced a rapid fall in plasma glucose concentrations , which was mirrored by an increase in plasma corticosterone concentrations . - pubmed
Moreover , the synergistic interaction of gemcitabine and paclitaxel was correlated to a block of the cells in the G0 / G1 compartment of cell cycle and to an increase of apoptotic cells compared to each drug . - pubmed
Glyceroneogenesis is a phosphoenolpyruvate carboxykinase ( PEPCK)-dependent process by which glycerol can be produced from precursors like glutamine and therefore represents a truncated gluconeogenic pathway . - pubmed
However , the demonstration that PEPCK is massively expressed in these larval forms as compared to adult parasites , together with the observation that 3 - mercaptopicolinate , a specific inhibitor of PEPCK , significantly reduces the effect of glutamine on sporocyst growth , suggest that glutamine could also be used for glucose or glycerol production . - pubmed
Results of [ 14C ] glutamine incorporation confirmed that neosynthesis of glucose and mainly of glycerol occurred in sporocysts and was dependent on PEPCK activity . - pubmed
Apart from light and melatonin little is known about other systems from the periphery that may provide information to the SCN . - pubmed
BACKGROUND : The oral dual endothelin ( ET ) antagonist bosentan has been established as a cornerstone in the treatment of pulmonary arterial hypertension ( PAH ) . - pubmed
Considering the importance of cAMP and cGMP to disparate physiological functions including visual response , smooth muscle relaxation , platelet aggregation , immune response , and cardiac contractibility , perhaps the 200 or more splice isoforms of PDE genes also play a major functional role . - pubmed
In addition , we used a PPAR - gamma antagonist , T0070907 , to determine the role of PPAR - gamma during ischemia . - pubmed
Both DNA binding and lipoprotein lipase expression were increased by the addition of the PPAR - gamma agonist rosiglitazone . - pubmed
Finally , we found that rosiglitazone - mediated protection after stroke was reversed by the PPAR - gamma antagonist T0070907 . - pubmed
Interestingly , infarction size was also increased by T0070907 in the absence of PPAR - gamma agonist , suggesting that endogenous PPAR - gamma ligands may mitigate the effects of cerebral ischemia . - pubmed
3 - Beta - hydroxysteroid dehydrogenase1 ( HSD3B1 ) and uridine diphosphate - glucuronosyltransferase 2B17 ( UGT2B17 ) are enzymes that inactivate dihydrotestosterone in the prostate and may affect dihydrotestosterone concentration in prostatic tissue . - pubmed
The activities of these enzymes were repressed by excess phosphate ( 4 mM ) in the culture medium , but this repression was reversed upon the onset of phosphate starvation in low phosphate defined medium ( LPDM ) containing 0.2 mM of phosphate at approximately the end of the exponential growth phase . - pubmed
The expressions of the two enzymes were also influenced by different carbon sources , including glucose , fructose , maltose , glycerol , and sodium acetate at different levels . - pubmed
The APase activity was derepressed most profoundly in LPDM containing fructose as a sole carbon source . - pubmed
However , the repression / derepression of the enzyme by phosphate was not observed in media containing glycerol or sodium acetate . - pubmed
The highest levels of PLase activity were detected in LPDMsodium acetate , followed by LPDM - fructose . - pubmed
PLase was not fully repressed by high phosphate concentrations when sodium acetate was utilized as the sole carbon source . - pubmed
Cytosensing and evaluation of cell surface glycoprotein based on a biocompatible poly ( diallydimethylammonium ) doped poly ( dimethylsiloxane ) film . - pubmed
To escape from attack of lymphocytes , villous trophoblasts do not express classical major histocompatibility complex ( MHC ) class I molecules . - pubmed
A lipidomics approach revealed that At4g24160 has additional triacylglycerol lipase and phosphatidylcholine hydrolyzing enzymatic activities . - pubmed
Gene alyPI was composed of a 1575 bp open reading frame encoding a protein of 57.4 kDa containing 524 amino acid residues with a signal peptide of 23 amino acids . - pubmed
AlyPI was most active at 40 degrees C and pH 7.0 in the presencce of 0.1 mol / L NaCl and stable over a broad range of pH , 6.0 - 10.6 . - pubmed
Another nine amino acid consensus region , YXRSELREM , only found in G - specific alginate lyases previously existed in AlyPI , which could degrade sodium alginate , M blocks , and G blocks and appeared to be a broad substrate - specific alginate lyase . - pubmed
The effects of selective antagonists of the four adenosine receptor subtypes were investigated on the airways responses of sensitized guinea - pigs to inhaled ovalbumin to ascertain the role of adenosine in these allergen responses , and compared with a corticosteroid , dexamethasone . - pubmed
Airway reactivity to inhaled histamine ( 1 mM ) and inflammatory cell influx in bronchoalveolar lavage fluid were also determined 24 h after ovalbumin challenge . - pubmed
ZM241385 ( A ( 2A ) receptor antagonist ) did not affect these responses , whereas DPCPX ( A ( 1 ) receptor antagonist ) exerted a small inhibition only of the LAR . - pubmed
MRS1706 ( A ( 2B ) receptor antagonist ) inhibited the airways hyperreactivity and cellular influx and enhanced the EAR . - pubmed
MRS1220 ( A ( 3 ) receptor antagonist ) inhibited the airways hyperreactivity and cellular influx without affecting EAR and LAR . - pubmed
Dexamethasone inhibited the ovalbumin - induced late asthmatic responses , airways hyperreactivity and cellular influx . - pubmed
The blockade of airway hyperreactivity and inflammatory cell influx by A ( 2B ) and A ( 3 ) receptor antagonists suggests that endogenous adenosine is released by inhaled allergen and these responses are mediated via A ( 2B ) and A ( 3 ) receptors in guinea - pigs . - pubmed
The adenosine released by allergen inhalation does not , however , appear to be involved in the EAR , but it may contribute to the LAR via A ( 1 ) receptors . - pubmed
Here , we further characterize the mechanisms mediating this low frequency stimulation ( LFS)-induced LTP in area CA1 of intact , urethane - anesthetized preparations . - pubmed
Local application of the protein synthesis inhibitor anisomycin in CA1 blocked LFS - induced LTP , as did application of H89 , an inhibitor of protein kinase A. - pubmed
A generalized model for correlation of the CD spectra of 2 - cyclohexenones with their structures is presented . - pubmed
Docosahexaenoic acid derivative prevents inflammation and hyperreactivity in lung : implication of PKC - Potentiated inhibitory protein for heterotrimeric myosin light chain phosphatase of 17 kD in asthma . - pubmed
Moreover , treatment with CRBM-0244 resulted in a decrease in NF - kappaB activation and cyclooxygenase-2 ( COX-2 ) overexpression triggered by TNF - alpha . - pubmed
The inhibition of peroxisome proliferator - activated receptor - gamma with GW9662 abolished the CRBM-0244 - mediated anti - inflammatory effects . - pubmed
CRBM-0244 reduced the Ca ( 2 + ) sensitivity of bronchial smooth muscle through a decrease in the phosphorylation and expression of the PKC - potentiated inhibitory protein for heterotrimeric myosin light chain phosphatase of 17 kDa ( CPI-17 ) . - pubmed
Furthermore , the presence of specialized enzymes such as 5 - lipoxygenase and 15 - lipoxygenase in the lung may convert CRBM-0244 into active mediators , leading to the resolution of inflammation . - pubmed
After oral administration of CRBM-0244 , airway leukocyte recruitment , airway mucus , ovalbumin - specific IgE , and proinflammatory markers such as TNF - alpha and COX-2 were markedly reduced . - pubmed
Hence , CRBM-0244 treatment prevents airway hyperresponsiveness , Ca ( 2 + ) hypersensitivity , and the overexpression of CPI-17 in lung tissue . - pubmed
This study characterized the UGT enzyme selectivity of niflumic acid ( NFA ) . - pubmed
Higher NFA concentrations ( 50 - 100 muM ) inhibited UGT1A1 and UGT2B15 but had little effect on the activities of UGT1A3 , UGT1A4 , UGT1A6 , UGT2B4 , UGT2B7 , and UGT2B17 . - pubmed
Consistent with previous reports , APAP was glucuronidated by recombinant UGT1A1 , UGT1A6 , UGT1A9 , and UGT2B15 . - pubmed
NFA concentrations in the range of 2.5 to 100 muM inhibited APAP glucuronidation by UGT1A1 , UGT1A9 , and UGT2B15 but not by UGT1A6 . - pubmed
The mean V(max ) for APAP glucuronidation by HLM was reduced by 20 , 35 , and 40 % , respectively , in the presence of 2.5 , 50 , and 100 muM NFA . - pubmed
Taken together , the NFA inhibition data suggest that UGT1A6 is the major enzyme involved in APAP glucuronidation . - pubmed
By electrochemical junction with iodide / iodine based redox electrolyte , perovskite QD - sensitized 3.6 mum - thick TiO ( 2 ) film shows maximum external quantum efficiency ( EQE ) of 78.6 % at 530 nm and solar - to - electrical conversion efficiency of 6.54 % at AM 1.5 G 1 sun intensity ( 100 mW cm(-2 ) ) , which is by far the highest efficiency among the reported inorganic quantum dot sensitizers . - pubmed
Thereby , flow cytometry revealed high cellular association and internalization of anti - IGF1 - R immunoliposomes ( soy phosphatidylcholine ( SPC)/cholesterol ( Chol)-polyethyleneglycol ( PEG)-1H7 , 50.1 + -2.2 % ) . - pubmed
Surface enhanced fluorescence ( SEF ) effect of acridine orange fluorophore in the proximity of silver nanoparticles ( NPs ) has been investigated experimentally in the aqueous solution system . - pubmed
Compared to the normal population , the levels of oxidized low - density lipoprotein ( LDL ) cholesterol are higher and the levels of high density lipoprotein ( HDL ) cholesterol are lower . - pubmed
Structure and dynamics of the two amphipathic arginine - rich peptides RW9 and RL9 in a lipid environment investigated by solid - state NMR and MD simulations . - pubmed
However , amino acids present in the carboxy - terminal region of CD5 , were necessary for this effect , indicating that ITAM - independent sites were involved in the interaction of c - Cbl with CD5 . - pubmed
Interactions between oestrogen and the renin angiotensin system - potential mechanisms for gender differences in Alzheimer ' s disease . - pubmed
Interactions between oestrogen and the renin angiotensin system ( RAS ) are reviewed and explored from the perspective where these interactions may modulate risk of developing Alzheimer ' s disease ( AD ) . - pubmed
Specifically , oestrogen prevents the production and action of angiotensin II ( Ang II ) , thought to exert harmful effects of the RAS in both hypertension and AD , while also potentially facilitating RAS - mediated Abeta degradation . - pubmed
Clinical trials targeting either the RAS or oestrogen systems for AD prevention and treatment should perhaps give closer attention to key biochemical components of these pathways as potential confounders to primary and secondary outcome measures . - pubmed
In a mesocosm experiment unfiltered seawater from the northern Baltic Sea was fertilized with inorganic nutrients and humic carbon ( CNP ) , and only with inorganic nutrients ( NP ) . - pubmed
The use of atazanavir , compared with other protease inhibitors , was associated with a lower likelihood of having a prolonged QTc . - pubmed
The highest in vitro antioxidant activity determined by DPPH radical scavenging was achieved with rutin hydrolyzed by hesperidinase . - pubmed
There was no statistical difference between xanthine oxidase inhibition by rutin before and after hydrolysis . - pubmed
pylori , in vitro , using a new photosensitising material ( PSM ) based on a ruthenium ( II ) complex covalently bound to micrometric glass beads . - pubmed
pylori isolates ( classified according to cagA genotype , and metronidazole - clarithromycin resistance ) were used . - pubmed
Subjects were classified as normoalbuminuric ( urinary albumin - to - creatine ratio [ UACR ] & lt;30 mg / g ) , microalbuminuric ( UACR & gt;=30 and & lt;300 mg / g ) , or proteinuric ( UACR & gt;=300 mg / g ) . - pubmed
They also presented greater measures of body mass index and waist circumference , higher values of total and LDL cholesterol , and lower values of HbA1c and serum creatinine than subjects with microalbuminuria or proteinuria . - pubmed
Molecular imaging and pharmacokinetics of ( 99 m ) Tc - hTERT antisense oligonucleotide as a potential tumor imaging probe . - pubmed
The uptake kinetics and imaging results of ( 99 m ) Tc - hTERT antisense oligonucleotides ( ASON ) in hTERT - expressing cells were examined in vitro and in vivo . - pubmed
The pharmacokinetics and acute toxicity studies of ( 99 m ) Tc - hTERT ASON were also performed . - pubmed
Abstract Background : Nephrotoxicity is a dose - limiting side effect of long - term use of tenofovir , a reverse transcriptase inhibitor that is used for the treatment of human immunodeficiency virus ( HIV ) infection . - pubmed
Interleukin-6 ( IL-6 ) and thiobarbituric acid reactive substances ( T - BARS ) were used as markers of inflammation and oxidative stress respectively . - pubmed
Exposure of cells to sphingosine 1 - phosphate induced pro - inflammatory responses characterized by interleukin-6 , interleukin-8 , and cyclooxygenase-2 up - regulations , as observed by ELISA and Western blot . - pubmed
Strikingly , cell treatment with sphingosine 1 - phosphate plus lipopolysaccharide resulted in the synergistic induction of cyclooxygenase-2 , and intercellular adhesion molecule 1 , as well as the secretion of prostaglandin E2 , the soluble form of the intercellular adhesion molecule 1 , and the pro - angiogenic factor vascular endothelial growth factor - A. - pubmed
As regards pro - osteogenic pathways , sphingosine 1 - phosphate induced calcium deposition and the expression of the calcification markers bone morphogenetic protein-2 and alkaline phosphatase , and enhanced the effect of lipopolysaccharide , an effect that was partially blocked by inhibition of sphingosine 1 - phosphate receptors 3 / 2 signaling . - pubmed
fumigatus gliT , a strain that is hypersensitive to gliotoxin . - pubmed
fumigatus gliT when challenged with gliotoxin . - pubmed
fumigatus gliT challenged with exogenous gliotoxin . - pubmed
Similarly , Rankl expression peaked in marrow cells within 3 days of iron exposure ( 9.2 - fold ) . - pubmed
By contrast , TPPPS1 exhibited other effects , and it induced the highest amount of NO production , thereby indicating that TPPPS1 had the best antioxidant activity . - pubmed
Results indicated that TPPPS comprised three main components , among which , TPPPS1 mainly showed antioxidant effects , whereas TPPPS2 and TPPPS3 played key roles in immunomodulation , especially TPPPS3 . - pubmed
Moreover , BABA seed priming reduced malondialdehyde content in the seedlings and enhanced the accumulation of proline , total protein , total carbohydrate , nitrate reductase activity , and activities of antioxidant enzymes like guaiacol peroxidase and superoxide dismutase . - pubmed
Polymorphism of the CYP3A5 gene and its effect on tacrolimus blood level . - pubmed
OBJECTIVES : Tacrolimus is the cornerstone for immunosuppression in renal transplant and is metabolized by the cytochrome P 450 3A ( CYP3A ) subfamily of enzymes in the liver and small intestine . - pubmed
A polymorphism in intron 3 of the CYP3A5 gene affects the expression of this enzyme and tacrolimus trough blood levels . - pubmed
Mean tacrolimus level in the CYP3A5 * 1 /* 1 group was 5.154 ng / mL ( range , 4.42 to 6.5 ng / mL ) , CYP3A5 * 1 /* 3 group was 5.348 ng / mL ( range , 3.1 to 9.87 ng / mL ) , and CYP3A5 * 3 /* 3 group was 9.483 ng / mL ( range , 4.5 to14.1 ng / mL ) . - pubmed
Tacrolimus drug level correlated well with presence or absence of CYP3A5 polymorphisms . - pubmed
Therefore , CYP3A5 polymorphism analysis before renal transplant may help determine the optimal dose of tacrolimus in this population and prevent acute rejection episodes or tacrolimus toxicity . - pubmed
Herein , the synthesis of a supported Ni - Pt bimetallic nanocatalyst on mesoporous ceria by a one - pot evaporation - induced self - assembly method is reported . - pubmed
Phase / structural analysis by XRD , TEM , and Auger electron spectroscopy was conducted to gain insight into the excellent catalytic performance of the Ni - Pt / CeO2 catalyst . - pubmed
Fludarabine ( FLU)-based conditioning was associated with decreased graft failure ( odds ratio 0 21 , P = 0 01 ) , decreased early mortality ( hazard ratio 0 25 , P = 0 01 ) and improved 5 - year overall survival ( FLU 87 8 % [ standard error ( SE ) 5 1 % ] versus non - FLU 59 3 % [ SE 9 5 % ] , P = 0 01 ) . - pubmed
It shows that a non - irradiation and busulfan - free conditioning regimen can be used successfully , also in alternative donor SCT . - pubmed
18F - FDG PET / CT of Seminal Vesicle Metastasis From Ascending Colon Adenocarcinoma . - pubmed
This also highlights the role of F - FDG PET / CT to detect metastatic adenocarcinoma from colon in the genitourinary system . - pubmed
Patients were genotyped for functional variants on vitamin D synthetic pathway including GC ( rs4588 , rs7041 , rs22020 , rs2282679 ) , CYP2R1 ( rs2060793 , rs12794714 ) , CYP27B1 ( rs10877012 ) , and DHCR7 ( rs12785878 ) . - pubmed
Mutation analysis of SUOX revealed homozygous c.1200C & gt ; G mutations , resulting in an amino acid substitution of tyrosine to a stop codon ( Y400X ) . - pubmed
The completely demineralized dentine collagen blocks were then remineralized in a calcium chloride agarose hydrogel and a sodium hydrogen phosphate and fluoride agarose hydrogel . - pubmed
Starting from common commercially available materials , the titled cis / trans - tetraarylethenes can be conveniently obtained by a one - pot process under mild conditions . - pubmed
Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex - type N - glycan shield that hides most protein epitopes on HIV-1 envelope ( Env ) . - pubmed
Dorsal repulsive axon guidance protein ( Draxin ) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1 , but also directly binds the EGF domain of Netrin-1 through a 22 - amino - acid peptide ( 22aa ) . - pubmed
, past-30 - day cocaine use and intravenous opiate use ) , number of positive drug tests , and receiving any sanction / therapeutic response were associated with two - year post - program recidivism . - pubmed
We report here that VB1 promotes the protective immune response to limit the survival of MTB within macrophages and in vivo through regulation of peroxisome proliferator - activated receptor gamma ( PPAR - gamma ) . - pubmed
In addition , VB1 increases mitochondrial respiration and lipid metabolism and PPAR - gamma integrates the metabolic and inflammatory signals regulated by VB1 . - pubmed
Limonene Inhibits Methamphetamine - Induced Sensitizations via the Regulation of Dopamine Receptor Supersensitivity . - pubmed
These results suggest that limonene may ameliorate drug addiction - related behaviors by regulating postsynaptic dopamine receptor supersensitivity . - pubmed
The crystallite size and band gap values of SiO2 , TiO2 , NiS - SiO2 and Cr2S3 - TiO2 - 1 were obtained from XRD and UV - vis DRS analysis and found insignificant 44.22 , 54.11 , and 57.11 nm , and 8.9 , 3.2 , 3.0 , 2.7 eV , respectively . - pubmed
Folic acid ( FA ) is one of the biologic molecules which has been targeted overexpressed - folic acid receptor ( FR ) on the surface of cancer cells . - pubmed
Blockage of PAR-1 with the PAR-1 - specific antagonist , SCH79797 , significantly suppressed the activation of Rab5 , EEA-1 , and the ERK1 / 2 and AKT pathways and subsequently increased EV production and enriched EV cargo contents . - pubmed
The genes gyrA , gyrB , rrs , eis promoter and tlyA of 257 drug - resistant clinical isolates were sequenced to identify mutations that could be responsible for resistance against fluoroquinolones and second - line injectable drugs . - pubmed
Mechanistic study revealed that SAL - miat acted as a competitive endogenous RNA ( ceRNA ) that upregulated the expression of sirt1 by sponging miR-22 - 3p . - pubmed
Stress and motivation status after rehabilitation were determined by corticosterone concentrations ( ELISA ) and immunoreactivity of DeltaFosB ( immunohistochemistry ) in the nucleus accumbens , respectively . - pubmed
To investigate the motivation and stress related to the exercises , the expression of DeltaFosB in the nucleus accumbens and corticosterone concentration were compared after rehabilitation . - pubmed
Finally , living imaging and hematoxylin and eosin ( HE ) staining were used to measure the morphology and pathology of lung and liver cancer cell metastases , respectively . - pubmed
Our group previously demonstrated that sarcoma cell lines were insensitive to epidermal growth factor receptor ( EGFR ) inhibitor gefitinib monotherapy . - pubmed
Considering the positive regulation of tumour energy production by both the EGFR signalling and tumour metabolism pathways , this study aimed to investigate the effect and mechanisms of combination therapy using gefitinib and PENAO in sarcoma cell lines in vitro and in vivo . - pubmed
In addition , with the increase of the introduced B3 monomer , the oxidation stability of SPI-6FODA has been greatly improved . - pubmed
Herein , pore size controllable meso - TiO2 layers are prepared using spin coating of commercial TiO2 nanoparticle ( NP ) paste with added soft polymer templates ( SPT ) followed by removal of the SPT at 500 C. The SPTs consist of swollen crosslinked polymer colloids ( microgels , MGs ) or a commercial linear polymer ( denoted as LIN ) . - pubmed
The PDGF - BB - induced stimulation of Kir2.1 currents was blocked by the PDGF - BB receptor beta ( PDGF - BBRbeta ) inhibitor AG1295 and was not affected by the PDGF - BBRalpha inhibitor AG1296 . - pubmed
The PDGF - BB - induced stimulation of Kir2.1 currents was blocked by the protein kinase A inhibitor Rp-8 - CPT - cAMPs ; however , the antagonist of protein kinase B ( GSK690693 ) had marginal effects on current activity . - pubmed
The PDGF - BB - induced stimulation of Kir2.1 currents was enhanced by forskolin , an adenylyl cyclase ( AC ) activator , and was blocked by the AC inhibitor SQ22536 . - pubmed
We conclude that PDGF - BB increases Kir2.1 currents via PDGF - BBRbeta through activation of cAMP - PKA signaling in RASMCs . - pubmed
Fluctuation of sulfite reductase ( SiR ) activity , expression of MET5 and MET10 genes , and concentrations of sulfite and selenium were evaluated to support the population findings . - pubmed
A novel strategy to treat CADM with rapidly progressive interstitial lung disease is the Janus kinase inhibitor tofacitinib . - pubmed
Chronic arsenic exposure enhances metastatic potential via NRF2 - mediated upregulation of SOX9 . - pubmed
One proposed contributor to metastasis in NSCLC is nuclear factor ( erythroid - derived 2 )- like 2 ( NRF2 ) , a transcription factor with known oncogenic properties that has proved to be critical for arsenic carcinogenesis . - pubmed
Here , we demonstrate that chronic arsenic exposure enhances the invasive and migratory capacity of immortalized lung epithelial cells via NRF2 - dependent upregulation of SRY - box 9 ( SOX9 ) , another transcription factor linked with cell proliferation , epithelial - mesenchymal transition , and metastasis . - pubmed
MYCNOS1 silencing enhanced the response of retinoblastoma cells to topotecan but not carboplatin . - pubmed
Experiments were repeated with AS101 ( an IL-10 protein synthesis inhibitor ) , MS-275 ( a selective class 1 HDAC inhibitor ) , or both . - pubmed
Increase of the sialic acid - rich variants of the B beta- and gamma - chains of fibrinogen Zurich II . - pubmed
The effects of saralasin , an angiotensin II antagonist , on blood pressure and the renin - angiotensin - aldosterone system in normal and hypertensive subjects . - pubmed
Radioligand binding to forebrain and hindbrain membranes after 6 days treatment was examined using [ 3H ] yohimbine to measure the number of adrenergic binding sites and [ 3H]clonidine and [ 3H ] idazoxan to assess nonadrenergic imidazoline sites . - pubmed
At pH 7.25 , CqTX is as potent as TTX in blocking the sodium channel , with an ED50 of 3.8 nM. - pubmed
Platelet activating factor ( PAF ) is a phospholipid mediator of inflammation and vascular leakage that may be important in the etiology of asthma . - pubmed
PAF - induced vascular leakage was inhibited in a dose - dependent fashion by the PAF receptor blocker WEB 2086 . - pubmed
However , PAF - induced vascular leakage was not inhibited by blockade of cyclooxygenase / lipoxygenase , calmodulin , calcium channels , protein kinase C , histamine receptors , or by destruction of peptidergic sensory nerves . - pubmed
Recently developed monoclonal antibodies against V gamma and V delta gene products are useful reagents for the identification and isolation of gamma / delta+ T - cell subpopulations . - pubmed
It is suggested that an endogenous BR ligand down - regulating GABAA receptors is released during the bicuculline - evoked enhancement of neuronal activity and may contribute to the epileptiform discharge of motoneurons in the spinal cord . - pubmed
The purpose of this study was to determine ( a ) if the administration of DHT suppresses plasma prolactin levels or its nocturnal surge within 24 h after the treatment , ( b ) how soon after the commencement of treatment do the concentrations of DHT increase and progesterone levels decrease in the circulation , ( c ) the ultrastructural changes that occur in corpora lutea , and ( d ) the changes in luteal P-450 side - chain cleavage ( P-450scc ) enzyme and mRNA content upon DHT treatment . - pubmed
ESR spectra of spin - labelled cholestane ( CSL ) revealed that this steroid probe was incorporated into the AChR - rich membrane vesicles in regions which were to a certain extent enriched preferentially in the steroid , both in the presence and in the absence of local anaesthetics . - pubmed
Since the nitroxide group present in CSL is also a paramagnetic quencher of the intrinsic protein fluorescence , this property was used to characterize the AChR - steroid interactions . - pubmed
The quenching induced by CSL was sensitive both to AChR concentration and to the action of cholinergic agonists . - pubmed
In competition experiments , the ability of CSL to quench the AChR intrinsic fluorescence was markedly inhibited by benzocaine , tetracaine and QX-222 ( a quaternary trimethylammonium derivative of lidocaine ) , and was totally inhibited by procaine . - pubmed
The effectiveness of local anaesthetics in inhibiting CSL - induced quenching followed the order : procaine much greater than benzocaine approximately greater than tetracaine greater than QX-222 . - pubmed
The data can be interpreted in terms of a model requiring specific association sites for local anaesthetics on the hydrophobic surface of the AChR which at least partially overlap with those for steroids . - pubmed
After IV administration , ketamine kinetics followed a 3 - term exponential decrease , indicating rapid initial distribution of the drug to highly vascular tissues including the brain , followed by redistribution into less vascular tissues , and elimination . - pubmed
The synthesis and testing of E-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone and Z-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone as gamma - emitting ligands for the androgen receptor . - pubmed
Two iodinated steroids , E-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone and Z-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone were synthesized in a search for a gamma - emitting androgen that binds with high affinity to the androgen receptor . - pubmed
These 17 alpha - iodovinyl steroids were synthesized because many 17 alpha - substituents do not interfere markedly with binding to the androgen receptor and because similar analogs of other steroids , estrogens and progestins , have been shown to have the requisite properties for ligands to those receptors . - pubmed
Both of these potential ligands were tested for their ability to compete with [ 3H ] R1881 for binding to the androgen receptor in cytosols from prostate , hypothalamus and pituitary . - pubmed
In order to test the two ligands directly , they were both synthesized labelled with 125I and tested for binding to the androgen receptor in prostatic cytosol and in vivo for specific concentration in androgen responsive tissues . - pubmed
While on the basis of relative binding affinity , these 2 steroids appeared to be good candidates for androgen receptor ligands , neither were useful for this purpose . - pubmed
Although no specific gpNu1 binding to the left side of cos can be detected , this DNA segment does play a specific role since a cos fragment that does not include the left side or whose left side is replaced by non - cos sequences , is unable to bind gpNu1 unless either spermidine or IHF is present . - pubmed
Reduction of catalase by feeding 3 amino 1,2,4 triazole does not enhance the toxicity of phosphine in treated insects . - pubmed
In pharmacologic doses ( 1 g or more / day ) , alone or in combination with other lipid - lowering drugs , nicotinic acid lowers very low - density ( VLDL ) and low - density lipoprotein ( LDL ) levels , while concurrently increasing high - density lipoprotein ( HDL ) levels . - pubmed
Mass spectrometry of L - beta - aspartamido carbohydrates isolated from ovalbumin . - pubmed
In the process , we have discovered six new components in the L - beta - aspartamido carbohydrate fraction of ovalbumin . - pubmed
The bilirubin values were low in the control group and after selective proximal vagotomy without pyloroplasty , higher after selective proximal vagotomy with Finney 's operation , antecolic Billroth II operation with enteroanastomosis by Baum and Billroth I operation , maximally after retro- and antecolic Billroth II operation . - pubmed
Influence of metabolic inhibitors on ATP level and ATP - ase activity in blood platelets . - pubmed
All the data indicate that increased gluconeogenesis is an early response to metabolic acidosis and will facilitate ammonia production by utilization of glutamate which inhibits the glutaminase I enzyme . - pubmed
Evaluation of the reliability of hemoglobin determination by copper sulphate densitometry . - pubmed
More severe vena caval obstruction ( n = 10 ) decreased mean arterial pressure 13 + /- 2 mmHg , central venous pressure 1.3 + /- 0.3 mmHg , and heart rate 47 + /- 12 beats / min , and increased fetal plasma ACTH 1,047 + /- 448 pg / ml , cortisol 4.4 + /- 2.2 ng / ml , and vasopressin 47.9 + /- 24.2 pg / ml , but did not alter 11 - deoxycortisol . - pubmed
End - inflation plasma ACTH and vasopressin concentrations were significantly related to the induced decreases in mean arterial and central venous pressures , suggesting that the hormonal responses to vena caval obstruction were mediated by cardiovascular mechanoreceptors . - pubmed
By analogy with other steroid hormones , the production of better , and especially more specific , antibodies might be expected . - pubmed
This stimulation was most powerful at low concentrations of enzyme , or high concentrations of squalene . - pubmed
Epoxidase activity was destroyed by Triton X-100 , deoxycholate or Cu2 + , and partially inhibited by thiol reagents , rotenone and antimycin A. - pubmed
The enzyme was not inhibited by cyanide or by several inhibitors of cytochrome P-450 . - pubmed
A study has been made of the IMP or poly ( I ) interactions leading to substitution of X and Y ligands in binuclear platinum complexes of the general formula : [ X - Pt ( dien )] m +-( CH2 ) 6 -[( dien ) Pt - Y ] n + ( NO3 ) m + n , where dien stands for diethylenetriamine , X = H2O , Y = Br , m = 2 , n = 1 ( I ) , X = Y = H2O , m = n = 2 ( II ) , and X = Y = Br , m = n = 1 ( III ) . - pubmed
PVA had no effect on the responsiveness to phytohemagglutinin - P , whereas HB and BV enhanced the phytohemagglutinin - P responsiveness . - pubmed
It has been suggested that an inhibitory ECM containing chondroitin-6 - sulfate proteoglycan ( C-6S - PG ) and tenascin ( TN ) , which appears homogeneously in the core of the OB following afferent fiber arrival , helps position ingrowing olfactory axons in the prospective glomerular layer ( GL ) ( Gonzalez and Silver , 1992 ; Gonzalez et al . - pubmed
In the present study , we suggest that the formation of TN / C-6S - PG in the precise ring pattern around glomeruli appears to be dependent upon the presence of bundled olfactory axons . - pubmed
Significant proliferation was also induced by IL-6 ( 7 + /- 2.3 % of BRDU+ cells ; P = 0.006 ) , IL-3 ( 5.3 + /- 1.3 % ; P = 0.01 ) and PIXY-321 ( 5.4 + /- 1.6 % ; P = 0.02 ) . - pubmed
We have investigated the mechanisms involved in this cAMP - mediated regulation of c - myc expression . - pubmed
These data suggest that cyclic AMP downregulates c - myc expression by inhibition of transcriptional initiation . - pubmed
The major cytosolic phospholipase A2 isoform preferentially hydrolyzes plasmalogen substrate , possesses a pH optimum of 7.0 , and is chromatographically resolvable from a minor cytosolic calcium - independent phospholipase A2 isoform that hydrolyzes plasmenylcholine and phosphatidylcholine substrates at similar rates and possesses a pH optimum of 8.5 . - pubmed
The major cytosolic calcium - independent phospholipase A2 isoform was identified as a 40 - kD polypeptide after its 182,000 - fold purification by sequential column chromatographies to a final specific activity of 67 mumol / mg.min . - pubmed
The specific role of sterol carrier protein-2 ( SCP-2 ) in the transport of newly synthesized biliary cholesterol was evaluated by an in vivo antisense oligonucleotide approach . - pubmed
In vivo SCP-2 antisense treatment reduced and delayed the appearance of biliary [ 14C ] cholesterol . - pubmed
These results suggest that SCP-2 is necessary for the rapid transport of newly synthesized cholesterol into bile and that hepatocytes can induce SCP-2 expression according to the rate of biliary secretion of newly synthesized cholesterol . - pubmed
Cultured tumor cells released PTH and were calcium sensitive , producing 122 + /- 16 pg / microgram DNA of intact PTH ( mean + /- SEM ) at 0.5 mmol / L calcium compared with 26 + /- 2 pg / microgram DNA at 3.0 mmol / L calcium . - pubmed
However , we found that somatostatin ( 0.1 microgram / L ) in cultured tumor cells increased the release of intact PTH ( 123 + /- 19 versus 82 + /- 1 pg / microgram DNA , P & lt ; 0.05 ) and thus might have a negative effect on the HHM . - pubmed
The stereospecific enzymatic hydrolysis by an array of commercially available enzymes of the racemic methyl ester of LLU - alpha was investigated . - pubmed
Four hundred forty - two women and 341 men were surveyed at Panama City Beach , Florida , to assess the effects of gender , age , fraternity or sorority membership , and travel motivation on alcohol consumption and binge drinking during spring break . - pubmed
Under constant - flow conditions , intraarterial injections of endothelin-1 and sarafotoxin 6c induced biphasic changes in hind - limb perfusion pressure characterized by an initial decrease followed by a secondary increase in perfusion pressure . - pubmed
The vasodilator and vasoconstrictor components of the biphasic responses to endothelin-1 and sarafotoxin 6c were reduced by bosentan , and the endothelin receptor antagonist reduced baseline systemic arterial and hind - limb perfusion pressures . - pubmed
Bosentan decreased vasoconstrictor responses to lower doses of angiotensin II , whereas responses to higher doses of angiotensin II and responses to vasopressin , U46619 , BAY K8644 , norepinephrine , acetylcholine , bradykinin , levcromakalim , PGE1 , adrenomedullin , and calcitonin gene - related peptide were not altered . - pubmed
Vasoconstrictor responses to ET-1 were not altered by the angiotensin AT1 receptor antagonist DuP 532 or the AT2 receptor antagonist PD123,319 . - pubmed
The protein tyrosine kinase could phosphorylate exogenous substrate corresponding to the N - terminal peptide of p34cdc2 kinase but could not do so on poly ( Glu : Tyr ) . - pubmed
The enzyme could not be inhibited by genistein , staurosporine and dimethyl aminopurine , but could be inhibited by piceatannol which is a known competitive inhibitor of the peptide binding site of mammalian protein tyrosine kinases . - pubmed
The enzyme activity in the cytoplasm is uniquely inhibited by sodium orthovanadate ( IC50 = 20 microM ) which is a known protein tyrosine phosphatase inhibitor . - pubmed
Regenerating hepatocytes after partial hepatectomy induced MGMT transiently within 3 days ; however , the induction of MGMT was persistent for 2 weeks after DEN injection , and the combined treatment of DEN and partial hepatectomy maintained the elevated MGMT level for up to 4 weeks . - pubmed
The biological significance of the differential induction of MGMT during partial hepatectomy or DEN - induced carcinogenesis is not obvious ; however , further studies on possible posttranslational modifications of MGMT protein might shed some light on the functional aspect of MGMT induction . - pubmed
Comparison of the effects of DEPs , extracted DEPs , or extracts of DEPs has shown that the increase in GM - CSF release is mainly due to the adsorbed organic compounds and not to the metals present on the DEP surface because the metal chelator desferrioxamine had no inhibitory effect . - pubmed
Furthermore , radical scavengers inhibited the DEP - induced GM - CSF release , showing involvement of reactive oxygen species in this response . - pubmed
Moreover genistein , a tyrosine kinase inhibitor , abrogated the effects of DEPs on GM - CSF release , whereas protein kinase ( PK ) C , PKA , cyclooxygenase , or lipoxygenase inhibitors had no effect . - pubmed
PD-98059 , an inhibitor of mitogen - activated protein kinase , diminished the effects of DEPs , whereas SB-203580 , an inhibitor of p38 mitogen - activated protein kinase , had a lower effect , and DEPs did actually increase the active , phosphorylated form of the extracellular signal - regulated kinase as shown by Western blotting . - pubmed
In addition , cytochalasin D , which inhibits the phagocytosis of DEPs , reduced the increase in GM - CSF release after DEP treatment . - pubmed
Reactive oxygen species and tyrosine kinase(s ) may be involved in the DEP - triggered signaling of the GM - CSF response . - pubmed
The demonstration of the presence of the elastin receptor on leukocytes facilitated the detailed description of the transmission pathway from receptor to the intracellular sites activated by the receptor : modifications of ion fluxes , increase of elastase production and excretion of reactive oxygen species , superoxide and NO * . - pubmed
The calcium transients triggered by elastin peptides acting on the receptor decrease with age , the receptor appears to be uncoupled from the G - proteins , but superoxide release is increased . - pubmed
The reactions required NADPH as a cofactor and were significantly inhibited by the various inhibitors of cytochrome P450 ( i.e. - pubmed
The results indicate that the degraded products of OPB-2045 are produced by C - C bond cleavage after monohydroxylation , dihydroxylation , and ketol formation at the site of the octyl side chain with possible involvement of cytochrome P450 systems . - pubmed
The two URA3 alleles were sequentially inactivated using the MPA(R)-flipping strategy , which is based on the selection of integrative transformants carrying a mycophenolic acid ( MPA ) resistance marker that is subsequently deleted again by site - specific , FLP - mediated recombination . - pubmed
When MPA(R ) was used as a reporter gene , it conferred an MPA - resistant phenotype on opaque but not white cells in strains expressing it from the SAP1 promoter , which was monitored at the level of single cells by a significantly enlarged size of the corresponding colonies on MPA - containing indicator plates . - pubmed
Similarly , white but not opaque cells became MPA resistant when MPA(R ) was placed under the control of the WH11 promoter . - pubmed
Signal transduction from RAGE to NF - kappaB involved the generation of reactive oxygen species , since reporter gene expression was blocked with the antioxidant N - acetyl - L - cysteine . - pubmed
CML - modified albumin produced rapid transient activation of tyrosine phosphorylation , extracellular signal - regulated kinase 1 and 2 , and p38 mitogen - activated protein kinase ( MAPK ) , but not c - Jun NH ( 2 )- terminal kinase . - pubmed
RAGE - mediated NF - kappaB activation was suppressed by the selective p38 MAPK inhibitor SB203580 and by coexpression of a kinase - dead p38 dominant - negative mutant . - pubmed
The PKC inhibitors staurosporine and calphostin C inhibited the increase in force/[Ca ( 2 +)]i induced by phenylephrine to a much greater extent than that induced by 80 mM K + . - pubmed
LY379196 , a specific PKCbeta inhibitor , did not inhibit the increase of calcium sensitivity due to phenylephrine . - pubmed
Whole - mount X - gal staining of 9.5 dpc embryos indicated Cre - mediated lacZ expression in forebrain , hindbrain , and along the midbrain flexure . - pubmed
Serum and liver samples obtained from dams ( GD 20 and PND 22 ) , fetuses ( GD 20 ) , and offspring ( PNDs 4 , 14 , 36 , and 90 ) were analyzed for circulating total serum thyroxine ( T(4 ) ) and triiodothyronine ( T(3 ) ) , or hepatic microsomal ethoxy - and pentoxy - resorufin - O - deethylase ( EROD and PROD ) , and uridine diphosphoglucuronosyl transferase ( UDPGT ) activity . - pubmed
Induction of PROD was similar in both dams and offspring ; however , EROD and UDPGT induction were much greater in offspring compared to dams ( EROD = 3.8 - fold ; UDPGT = 0.5 - fold ) . - pubmed
Immunoglobulin G - class mouse monoclonal antibodies to major brain gangliosides . - pubmed
Enzyme - linked immunosorbent assay ( ELISA ) screening against 14 closely related synthetic and purified gangliosides confirmed the high specificity , with no significant cross - reactivity except that of the anti - GD1a mAbs for the closely related minor ganglioside GT1a alpha . - pubmed
Lysine 270 in the third intracellular domain of the oxytocin receptor is an important determinant for G alpha ( q ) coupling specificity . - pubmed
Substitution of the 2i domain of OTR into V(2)R decreased ligand affinity and vasopressin - stimulated adenylyl cyclase activity and only slightly increased phosphatidylinositide turnover . - pubmed
In contrast , substitution of the OTR3i domain into V(2)R produced a receptor chimera with high ligand affinity , decreased vasopressin - stimulated adenylyl cyclase activity , and markedly enhanced ligand - stimulated phosphatidylinositide turnover . - pubmed
The C - terminal 36 amino acids , but not the N - terminal 13 amino acids , of the OTR3i domain contained the determinants critical for enhanced activation of PLC . - pubmed
Mutation of a single lysine in the C - terminal OTR3i sequence to the corresponding V(2)R residue ( valine ) eliminated the enhanced ability of the V(2)R chimera to stimulate PLC but did not affect maximal adenylyl cyclase stimulation . - pubmed
Furthermore , mutation of this residue ( K270 ) in wild - type OTR completely abolished the ability of the receptor to stimulate phosphatidylinositide turnover , with only a small reduction in ligand affinity . - pubmed
These data demonstrate that OTR K270 is critically important in the stimulation by OTR of phosphatidylinositide turnover and that this determinant can also increase this activity in the V(2)R chimera . - pubmed
A pH dependence study on the unfolding and refolding of apoazurin : comparison with Zn ( II ) azurin . - pubmed
Previous folding studies have shown that the equilibrium denaturation of the holoprotein follows a two - state process ; however , upon removal of the copper , the denaturation had been reported to follow a three - state process . - pubmed
It is our contention that the second transition displayed in the denaturation curves previously reported for apoazurin arise from protein heterogeneity - in particular , from the presence of Zn ( II ) azurin . - pubmed
We extended our investigation into the denaturation of Zn ( II ) azurin at pH 6.0 and 7.5 . - pubmed
Further , comparing the denaturation curves of Zn ( II ) azurin measured by circular dichroism with those measured by fluorescence indicates that the denaturation of Zn ( II ) azurin is far less simple than the denaturation of apoazurin . - pubmed
Gastric luminal and serum gastrin levels and gastric mucosa and tumor PGE2 , which were greatly elevated before eradication , became normalized after this procedure . - pubmed
It shares less than 20 % amino acid sequence identity with other lipases for which there are crystal structures , and shows significant insertions compared with the typical alpha / beta hydrolase canonical fold . - pubmed
The structure contains a zinc - binding site which is unique among all lipases with known structures , and which may play a role in enhancing thermal stability . - pubmed
In contrast , alpha - CT did not potentiate the twitch response or contractions induced respectively by a single pulse TNS or by direct electrical stimulation to the smooth muscle . - pubmed
The regimen outlined in this brief paper focuses on therapeutically utilizing iron and iron compounds in transferrin receptor - rich tumor cells in ways that inhibit and lyse same . - pubmed
Balanol analogues probe specificity determinants and the conformational malleability of the cyclic 3 ' , 5 '- adenosine monophosphate - dependent protein kinase catalytic subunit . - pubmed
Here , we characterize three balanol analogues that inhibit cyclic 3 ' , 5 '- adenosine monophosphate - dependent protein kinase ( PKA ) more specifically and potently than calcium - and phospholipid - dependent protein kinase ( PKC ) . - pubmed
Crystal structures of the PKA catalytic ( C ) subunit complexed to each analogue show the Gly - rich loop stabilized in an " intermediate " conformation , disengaged from important phosphoryl transfer residues . - pubmed
The balanol analogues serve to test the model and to highlight differences in the active site local environment of PKA and PKC . - pubmed
The PKA C subunit appears to tolerate balanol analogues with D - ring modifications ; PKC does not . - pubmed
A deglucosylated metabolite of paeoniflorin , paeoniflorgenin ( PG ) , in serum was identified based on HPLC / MS and NMR spectral data . - pubmed
Membrane microdomains enriched in glycosphingolipids , cholesterol , glycosylphosphatidylinositol - anchored proteins and Src - family kinases ( lipid rafts , GEMs ) appear to play many important roles , especially in immunoreceptor signaling . - pubmed
All these molecules possess a very short N - terminal extracellular peptide ( 4 - 17 amino acids ) , transmembrane segment followed by a palmitoylation motif ( CxxC ) and cytoplasmic domain containing up to 10 tyrosine residues potentially phosphorylated by the Src- or Syk - family kinases . - pubmed
Tyrosine - phosphorylated transmembrane adaptors bind ( directly via SH2 domains or indirectly ) other signaling molecules such as several cytoplasmic adaptors and enzymes . - pubmed
The number of AR - immunoreactive ( AR - ir ) cells was regulated by testosterone in medial amygdala and caudal arcuate , and by photoperiod in the medial preoptic nucleus and the posterodorsal medial amygdala . - pubmed
A significant interaction between androgen treatment and daylength in regulation of SRC-1 - ir was found in anterior medial amygdala . - pubmed
The present results indicate that daylength - induced fluctuations in SRC-1 and AR expression may contribute to seasonally changing effects of testosterone . - pubmed
The total increment of plasma homocysteine was assessed using the positive incremental area under the plasma homocysteine concentration curve over the 240 - minute post - methionine - loading period ( DeltaAUC(0 - 240 ) ) . - pubmed
Postoperatively , they exhibited decreased levels of interleukin ( IL )- 2 , interferon - gamma , IL-12 , granulocyte - macrophage colony - stimulating factor and IL-15 , low cellular immune responses ( AMLR , NK- and LAK - cytotoxicity ) and increased levels of IL-1beta , tumor necrosis factor - alpha , IL-6 , IL-10 and prostaglandin E ( 2 ) . - pubmed
Molecular basis of the low activity of antitumor anthracenediones , mitoxantrone and ametantrone , in oxygen radical generation catalyzed by NADH dehydrogenase . - pubmed
Synthetic antitumor anthracenedione drugs , in contrast to anthracycline antibiotics , are ineffective in free radical formation in NADH dehydrogenase system . - pubmed
Our results have indicated that neither the reduction potential nor the side chain conformation and the energies of border orbitals ( HOMO and LUMO ) determine the ability of anthracenediones to stimulate reactive oxygen species formation in NADH dehydrogenase system . - pubmed
It was shown that the distribution of the molecular electrostatic potential ( MEP ) , around the quinone system was crucial for this ability . - pubmed
We have found for non - stimulating anthracenediones that the clouds of positive MEP cover the quinone carbon atoms while for agents effective in stimulating reactive oxygen species formation the clouds of negative MEP cover continuously the aromatic core together with the quinone system . - pubmed
Among them , eight are new , namely as trichobrachin A I - IV ( Aib9 - Pro10 sequence ) and as trichobrachin B I - IV ( Val9 - Pro10 sequence ) . - pubmed
However , we provide evidence that a non - autonomous increase of Diap1 protein levels , an inhibitor of apoptosis , accounts for the suppression of hid . - pubmed
Furthermore , before they die , vps25 mutant clones trigger non - autonomous proliferation through a failure to downregulate Notch signaling , which activates the mitogenic JAK / STAT pathway . - pubmed
After osmotic rupture of the isolated amyloplasts and chloroplasts , a clear separation of the envelope - membranes was achieved by discontinuous sucrose density gradient centrifugation . - pubmed
BCR / ABL - positive leukemia cells accumulated more replication - dependent DNA double - strand breaks ( DSBs ) than normal counterparts after treatment with cisplatin and mitomycin C ( MMC , as assessed by pulse field gel electrophoresis ( PFGE ) and neutral comet assay . - pubmed
Elevated levels of drug - induced DSBs in leukemia cells were associated with higher activity of ATR kinase , and enhanced phosphorylation of histone H2AX on serine 139 ( gamma - H2AX ) . - pubmed
In addition , the expression and ATR - mediated phosphorylation of Chk1 kinase on serine 345 were often more abundant in BCR / ABL - positive leukemia cells than normal counterparts after genotoxic treatment . - pubmed
Inhibition of ATR kinase by caffeine but not Chk1 kinase by indolocarbazole inhibitor , SB218078 sensitized BCR / ABL leukemia cells to MMC in a short - term survival assay . - pubmed
i.p ) enhanced lipid peroxidation , hydrogen peroxide content , glutathione S - transferase and xanthine oxidase with reduction in the activities of hepatic antioxidant enzymes viz . - pubmed
, glutathione peroxidase , superoxide dismutase and caused depletion in the level of hepatic glutathione content . - pubmed
TAA treatment also enhanced tumor promotion markers , ornithine decarboxylase ( ODC ) activity and [ 3H ] thymidine incorporation into hepatic DNA . - pubmed
Spatial variations in electric conductivity and evolutions of band structures of polyaniline ( PANI ) films have been studied by use of a so - called current - sensing atomic force microscope ( CS - AFM ) or atomic force microscope current image tunneling spectroscopy ( AFM - CITS ) . - pubmed
Successful callus formation was obtained in all three species using Murashige - Skoog 's ( MS ) medium supplemented with 1 - naphthalene - acetic acid ( NAA ) ( 1 ppm ) and kinetin ( 0.1 ppm ) . - pubmed
Plantlet formation of C. ZEDOARIA from stem tips was successfully conducted on MS medium containing NAA ( 0 - 1 ppm ) or 6 - benzyladenine ( BA)(0 - 3 ppm ) at 26 degrees C under the condition of 12 h photoperiod . - pubmed
Exercise increases the distribution of leukotrienes and influences the leukotriene transcription pathway ; it could be shown that the genes ALOX5 and ALOX5AP encoding for 5 - lipooxygenase ( 5 - LO ) and 5 - lipoxygenase - activating protein ( FLAP ) as well as activators for 5 - LO , p38 - mitogen - activated protein kinase ( MAPK ) , and others , are enhanced after exercise in healthy subjects . - pubmed
Possibly EIA is triggered via leukotriene release if a predisposition or other conditions ( eg , epithelial injury and repair ) are present . - pubmed
Binding of the secreted axon guidance cue Slit to its Robo receptor results in inactivation of the neural , calcium - dependent cell - cell adhesion molecule N - cadherin , providing a rapid epigenetic mechanism for integrating guidance and adhesion information . - pubmed
Complex formation results in Abl - mediated phosphorylation of beta - catenin on tyrosine 489 , leading to a decrease in its affinity for N - cadherin , loss of N - cadherin function , and targeting of phospho - Y489 - beta - catenin to the nucleus . - pubmed
In addition , seeds of this and other mutants in the ethylene signaling pathway ( ctr1 - 1 , eto1 - 1 , etr1 - 1 , ein2 - 1 ) show different response to imbibition than the wild type . - pubmed
In contrast , in the seeds of the ethylene mutants , reduction in curvature values occurs in both basal and apical poles , and its shape remains as a prolate spheroid . - pubmed
Our data indicate that the ethylene signaling pathway is involved , in general , in the complex regulation of seed shape and , in particular , in the establishment of polarity in seeds , controlling curvature values in the seed poles . - pubmed
Exploratory trials are investigating the use of more complete estrogen suppression with luteinizing - hormone - releasing hormone agonists with aromatase inhibitors . - pubmed
In addition to these granuphilin - null phenotypes , Rab27a / granuphilin doubly deficient cells showed the decreases in granules located next to the docked area and in fusion from granules near the plasma membrane in the early phase of glucose - stimulated secretion , similar to Rab27a - mutated cells . - pubmed
Activin inhibitors ( Activin - M108A / SB431542 ) with differing specificities for the other TGFbeta members under consideration were applied during HESC decidualization in vitro . - pubmed
Spotlight on anti - CD25 : daclizumab in MS . - pubmed
One of these , the humanized monoclonal anti - CD25 antibody daclizumab ( Zenapax ) , is directed against the interleukin-2 ( IL-2 ) receptor alpha chain ( CD25 ) that is involved in clonal expansion of autoreactive T - cells by binding of its ligand IL - 2 . - pubmed
Following promising observations in uveitis , daclizumab has since been tested in a number of small clinical trials in MS based on the rationale that blocking CD25 would prevent the expansion of autoreactive T - lymphocytes . - pubmed
The chemoresistive sensors based on single poly ( pyrrole )- NTA chelator nanotube exhibited detection as low as one - hundredth attomolar ( 0.6 ppt ) cupric ions and 1 ng / ml of penta - histidine tagged syntaxin protein . - pubmed
C18 , C8 , and perfluoro reversed phases on diamond for solid - phase extraction . - pubmed
At the highest dosages used , FOA - PYR and FOA - DAP - ART combinations were synergistic with 100 % cure rate , while FOA - PYR - ART was antagonistic . - pubmed
[ Synthesis and alpha - glucosidase inhibitory activity of N -( 1,5 - diaryl-3 - pentone-1 - yl )- 4 - aminobenzoic acid ] . - pubmed
The MP structural gene ( lupA gene ) was cloned by inverse PCR , and the open reading frame of 1,443 bp encoded a 463 amino acid protein ( without signal peptide ) . - pubmed
Phosphatidylinositol- and phosphatidylcholine - transfer activity of PITPbeta is essential for COPI - mediated retrograde transport from the Golgi to the endoplasmic reticulum . - pubmed
Phosphatidylinositol transfer protein beta ( PITPbeta ) , an essential protein that possesses phosphatidylinositol ( PtdIns ) and phosphatidylcholine ( PtdCho ) lipid transfer activity is known to localise to the Golgi and ER but its role in these membrane systems is not clear . - pubmed
Depletion of PITPbeta leads to a decrease in PtdIns ( 4 ) P levels , compaction of the Golgi complex and protection from brefeldin - A - mediated dispersal to the ER . - pubmed
This phenotype can be rescued by expression of wild - type PITPbeta but not by mutants defective in docking , PtdIns transfer and PtdCho transfer . - pubmed
These data demonstrate that the PtdIns and PtdCho exchange activity of PITPbeta is essential for COPI - mediated retrograde transport from the Golgi to the ER . - pubmed
SOD-1 was directly associated with parasitaemia , plasma creatinine and alanine amino - transaminase levels , while TNF - alpha correlated only with the later enzyme . - pubmed
OBJECTIVE : This investigation assessed the effect of serum estradiol levels on outcomes of in - vitro fertilization and embryo transfer ( IVF ) cycles . - pubmed
MATERIALS AND METHOD : This was a retrospective cohort study of 1123 IVF cycles comparing impact of estradiol ( E(2 ) ) levels on follicular development , fertilization , embryo quality , implantation , pregnancy rate , miscarriage rate , and selected obstetric complications . - pubmed
[ Behcet ' s disease and associations with HLA - B5 antigen ( a review of literature and the authors ' findings ) ] . - pubmed
AIM : To estimate the distribution of HLA Class I ( A , B ) antigens in patients with Behcet ' s disease ( BD ) and the association of HLA - B5 antigen with the clinical manifestations of the disease in different ethnic and population groups in relation to gender . - pubmed
RESULTS : In patients with BD , the prevalence of HLA - B5 antigen proved to be significantly higher than that in the controls ( 72.0 and 21 % , respectively ) and to be similar in patients of different ethnicities living in the Caucasus and Transcaucasus ( 80 - 83 % ) while the number of HLA - B5 antigen - positive patients with BD was thrice less in the Russian population than in other BD patients ( p & lt ; 0.01 ) . - pubmed
This embodiment is superior to current SLN mapping agents such as nontargeted [ ( 99m ) Tc ] sulfur colloids and Isosulfan Blue , as well as the phase III targeted ligand [ ( 99m ) Tc]SPECT Lymphoseek counterpart , species that are visible by SPECT or visible absorbance separately . - pubmed
Facile incorporation of ( 18 ) F into a NIRF probe should promote many synergistic PET and NIRF combinations . - pubmed
In migraineurs , GTN administration was associated to a significant facilitation in temporal summation of pain ( reduced TST and increased painful sensation ) 60 ' , 120 ' and 180 ' after drug intake when compared to baseline , to placebo condition and to controls after GTN intake . - pubmed
The results indicated that caulophine and taspine were the target component acting on the epidermal growth factor receptor . - pubmed
In the last decade , PET / CT using radiolabeled fluorodeoxyglucose has been added to the imaging armamentarium , mostly for imaging lung cancer . - pubmed
Phosphorylation of a threonine residue ( Thr(11 ) ) in Cds1 by Rad3 occurs at a basal level in the absence of three other parallel Rad3 - dependent phosphorylations on the mediator Mrc1 and Rad9 in the checkpoint clamp complex . - pubmed
However , the three parallel Rad3 - dependent phosphorylations are all required for efficient phosphorylation of Thr(11 ) in Cds1 by Rad3 . - pubmed
Phosphorylation of Thr(11 ) has been shown previously to promote autophosphorylation of Thr(328 ) in the kinase domain of Cds1 , which directly activates the enzyme , leading to full activation of the checkpoint pathway . - pubmed
Through 2 - D gel electrophoresis and MALDI - TOF - TOF - based proteomics approaches , we found that 14 - 3 - 3beta , which was one of the proteins that were differentially expressed by 5 - fluorouracil - treated gastric cancer SC - M1 cells , was upregulated in gastric cancer cells . - pubmed
MTT score was higher in the albumin group than in the control group ( P<0.05 ) . - pubmed
As compared with that of the albumin group , MTT score and FN protein concentration decreased , FN mRNA significantly down - regulated in NCTD intervention groups respectively ( P<0.05 ) . - pubmed
Cd treatment induced a DNA hypermethylation , as well as an up - regulation of CMT , indicating that de novo methylation did indeed occur . - pubmed
The data demonstrate that Cd perturbs the DNA methylation status through the involvement of a specific methyltransferase . - pubmed
FINDINGS : We show that inhibition of Akt by the Akt inhibitor X enhanced the production of PGE2 and PGD2 without affecting the expression of COX-2 , mPGES-1 , mPGES-2 and cytosolic prostaglandin E synthase ( cPGES ) . - pubmed
In contrast , the mTOR inhibitor rapamycin enhanced both COX-2 and mPGES-1 immunoreactivity and the release of PGE2 and PGD2 . - pubmed
Interestingly , NVP - BEZ235 , a dual PI3K / mTOR inhibitor , enhanced COX-2 and reduced mPGES-1 immunoreactivity , albeit PGE2 and PGD2 levels were enhanced in LPS - stimulated microglia . - pubmed
CONCLUSION : Taken together , we demonstrate that blockade of mTOR and/or PI3K / Akt enhances prostanoid production and that PI3K / Akt , GSK-3 and mTOR differently regulate the expression of mPGES-1 and COX-2 in activated primary microglia . - pubmed
For preventing the scar contracture of host tissue and adjusting the tensile strength of covering cell sheets , a controlled collagen crosslinking step process in the preparation of skin - fibroblast sheets for repairing wound was investigated by using beta - aminopropionitrile ( BAPN ) , a collagen crosslinking inhibitor , in the culture medium . - pubmed
Collagen fibres of control cell sheets were more dense and thick than those of BAPN - treated cell sheets , where the crosslinking of collagen fibres was clearly inhibited . - pubmed
Sodium dodecyl sulphate - polyacrylamide gel electrophoresis ( SDS - PAGE ) showed that BAPN - treated cell sheets gave no beta - chain band , indicating that the collagen crosslinkings of the fibroblast sheets were able to be controlled by BAPN . - pubmed
Serum levels of lactate dehydrogenase , blood urea nitrogen , and creatinine were elevated . - pubmed
However , the amounts of ZO-1 mRNA and protein in the corneal epithelium were not affected by BAC treatment . - pubmed
In fact , if the respiratory chain were present in the myelin membrane , then the ATP synthase would function in reverse , hydrolyzing rather than synthesizing ATP . - pubmed
Polyphenols with a catechin configuration were better DHFR inhibitors than those with an epicatechin configuration . - pubmed
Derivatives with a hydroxyl group para on the ester - bonded gallate moiety presented a high in vitro binding to DHFR , but exhibited transport problems in cell culture due to ionization at physiologic pHs . - pubmed
The impact of the binding of catechins to serum albumin on their biological activity was also evaluated . - pubmed
The data suggest that changes in their structure to avoid serum albumin interactions and to facilitate plasmatic membrane transport are essential for the intracellular functions of catechins . - pubmed
The study investigated whether successful transfer of game - based cognitive improvements to untrained tasks might be modulated by preexisting neuro - developmental factors , such as genetic variability related to the catechol - O - methyltransferase ( COMT)-an enzyme responsible for the degradation of dopamine . - pubmed
We trained participants , genotyped for the COMT Val(158)Met polymorphism on playing " Half - Life 2 " , a first - person shooter game which has been shown to improve cognitive flexibility . - pubmed
In addition , the applicability of electrochemical oxidation as an advanced post - treatment for the complete removal of chemical oxygen demand ( COD ) from the anaerobically treated cheese whey was evaluated . - pubmed
When the anaerobic reactor effluent was post - treated over a boron - doped diamond ( BDD ) anode at 9 and 18 A and in the presence of NaCl as the supporting electrolyte , complete removal of COD was attained after 3 - 4 h of reaction . - pubmed
In the present study , a new kind of electroneutralized triblock copolymer , LPL , dendron G2(L - lysine - Boc )- PEG2k - dendron G2(L - lysine - Boc ) , was designed and investigated . - pubmed
To evaluate knee intraarticular adhesion , we performed macroscopic evaluation , histological and collagen density analyses , hydroxyproline content determination , fibroblast counting and densitometric analyses . - pubmed
The results showed that loose collagen tissues with little or no adhesion were present around the decorticated areas in the group treated with 0.5 mg / ml colchicine . - pubmed
Moreover , ACF decreased Bcl-2 expression and increased Bax expression . - pubmed
Conversely , the ovarian zone exhibited a stable increase pattern which was similar to the plasma 17beta - estradiol ( E2 ) levels . - pubmed
Through focused screening of our compound collection , we identified a series of N - sulfonylated benzoxazepines , which displayed micromolar affinity for the RORgamma ligand - binding domain ( LBD ) in a radioligand binding assay . - pubmed
A cocrystal structure of inverse agonist 15 ( 2 - chloro-6 - fluoro - N -( 4 -{[ 3 -( trifluoromethyl ) phenyl ] sulfonyl }- 2,3,4,5 - tetrahydro-1,4 - benzoxazepin-7 - yl ) benzamide ) bound to the RORgamma - LBD illustrated that both hydrophobic interactions , leading to an induced fit around the substituted benzamide moiety of 15 , as well as a hydrogen bond from the amide NH to His479 seemed to be important for the mechanism of action . - pubmed
This structure is compared with the structure of agonist 25 ( N -( 2 - fluorophenyl )- 4 -[( 4 - fluorophenyl ) sulfonyl ]- 2,3,4,5 - tetrahydro-1,4 - benzoxazepin-6 - amine ) and structures of other known RORgamma modulators . - pubmed
Emerging evidence suggest that upregulation of arginase is of central importance for reduced NO bioavailability due to competition for the substrate L - arginine between arginase and the endothelial form of NO synthase . - pubmed
Arginase is also associated with increased oxidative stress , further impairing NO bioavailability . - pubmed
RESULTS : The serum glucose concentration decreased , the insulin resistance and insulin sensitivity significantly increased in treated groups . - pubmed
CONCLUSIONS : Swimming exercises accompanied by consuming UD aqueous extracts effectively improved diabetic parameters , repaired pancreatic tissues in streptozotocin - induced diabetics in vivo , and increased glucose uptake or insulin in UD - treated cells in vitro . - pubmed
There was no statistically significant difference in hemoglobin , iron , hepcidin , and sTfR concentration between the two studied groups . - pubmed
There have been reports of successful treatment combining doxycycline 100 mg and montelukast 10 mg , dosed BID for 3 months . - pubmed
Recent randomized trials demonstrate reduced ischemic events including myocardial infarction ( MI ) , stroke , and death with continued DAPT for up to 30 months or longer , particularly in the post - MI population . - pubmed
Among isolates phenotypically identified as MSSA by antimicrobial susceptibility assays , we verified 30 oxacillin - susceptible isolates , which exhibited the mecA gene , without mec complex amplification and were thus classified as OS - MRSA . - pubmed
OBJECTIVES : Celecoxib is a cyclooxygenase-2 - specific inhibitor indicated to treat acute pain and pain secondary to osteoarthritis and rheumatoid arthritis . - pubmed
The occurrence , which is dependent on the presence of epidermal growth factor , of new Raman features associated with the appearance of phosphothreonine and phosphoserine residues reflects a signal transduction from the membrane to the nucleus , with concomitant modification of DNA / RNA structural characteristics . - pubmed
The community composition and abundance of aerobic / anaerobic ammonia / ammonium - oxidizing microorganisms ( AOM ) in the aquitard were investigated in this study . - pubmed
The alpha subunit of ammonia monooxygenase gene ( amoA ) was used as the biomarker for the detection of aerobic ammonia - oxidizing archaea ( AOA ) and bacteria ( AOB ) , and also partial 16S rRNA gene for Plantomycetes and anaerobic ammonium - oxidizing ( anammox ) bacteria . - pubmed
The Pearson moment correlation analysis showed that AOA amoA gene abundance positively correlated with pH and ammonium concentration , whereas AOB amoA gene abundance negatively correlated with C / N ratio . - pubmed
This is the first report that highlights the presence with low diversity of AOM communities in natural aquitard of rich ammonium . - pubmed
Pre and postmeal samples were analysed for total bile acids , parathyroid hormone and C - terminal telopeptide . - pubmed
Results Body weight loss post Roux - en - Y gastric bypass was associated with a median 4.9 - fold increase in peak postprandial total bile acid concentration , and a median 2.4 - fold increase in cumulative food evoked bile acid response . - pubmed
These results suggest that post - Roux - en - Y gastric bypass increases in total bile acids do not effectively oppose an ongoing resorptive signal operative along the gut - bone axis . - pubmed
Intracoronary adenosine - induced ventricular arrhythmias during fractional flow reserve ( FFR ) measurement : case series and literature review . - pubmed
It is based on the change in the pressure gradient across the stenosis after the achievement of maximal hyperemia of the coronary circulation which is commonly induced by intravenous ( IV ) or intracoronary ( IC ) administration of adenosine . - pubmed
Here , we have described three cases of IC adenosine - induced ventricular arrhythmias during FFR measurement from our institution , and after literature review we found that all the cases of ventricular arrhythmias induced by adenosine during FFR measurement were observed where it was administered via IC route . - pubmed
Although a causal relationship between the use of IC adenosine during FFR measurement and the induction of ventricular arrhythmias is not yet established , we suggest using IV adenosine as the preferred route of administration until we better understand the incidence and mechanism underlying this phenomenon . - pubmed
We showed that the migration of hDPSCs was induced by SDF-1 in a concentration - dependent manner and could be inhibited with siCXCR4 ( C - X - C chemokine receptor type 4 ) and siCDC42 ( cell division control protein 42 ) , as well as drug inhibitors such as AMD3100 ( antagonist of CXCR4 ) , LY294002 ( inhibitor of PI3 K ) and PF573228 ( inhibitor of FAK ) . - pubmed
OBJECTIVE : To compare subjective , objective and safety performance of silicone hydrogel ( SiHy ) daily disposable ( DD ) with hydrogel ( Hy ) DD contact lenses . - pubmed
Reversible janus associated kinase ( JAK ) inhibitors such as tofacitinib and decernotinib block cytokine signaling and are efficacious in treating autoimmune diseases . - pubmed
Verapamil , a P - glycoprotein inhibitor , significantly increased the absorption of 4,8 - sphingadienine but did not affect the absorption of sphingosine . - pubmed
They both negatively regulate OprD porin expression which affects the intake of antibiotics like carbapenems . - pubmed
More specifically , Lico B induced apoptosis through the regulation of specificity protein 1 and apoptosis - related proteins including CCAAT / enhancer - binding protein homologous protein , death receptors , and poly ( ADP - ribose ) polymerase . - pubmed
In vitro , primary chondrocytes were treated with corticosterone ( 0 - 1250 nM ) , caffeine ( 0 - 100 muM ) , mitogen - inducible gene 6 ( Mig-6 ) siRNA and epidermal growth factor receptor ( EGFR ) siRNA , respectively , or together . - pubmed
Furthermore , PCE upregulated Mig-6 and suppressed EGFR expression in the HZ . - pubmed
In vitro , a high - concentration corticosterone ( 1250 nM ) upregulated Mig-6 expression , inhibit EGFR / c - Jun N - terminal kinase ( JNK ) signaling pathway and terminal differentiation genes expression in chondrocytes and reduced cell apoptosis , and these above alterations could be partly reversed step - by - step after Mig-6 and EGFR knockdown . - pubmed
Ipragliflozin treatment resulted in statistically significant improvements versus baseline in hemoglobin A1c , fasting plasma glucose concentration , body weight , blood pressure , heart rate , and serum concentrations of low - density lipoprotein cholesterol and triglycerides . - pubmed
One pot biocatalytic synthesis of a biodegradable electroactive macromonomer based on 3,4 - ethylenedioxytiophene and poly ( l - lactic acid ) . - pubmed
All macromonomers ( EDOT - PLLA ) were characterized by 1H and 13C RMN , MALDI - TOF , GPC and EDX . - pubmed
However , in the case of established persistent viral infection , sustained elevation of IFN - I expression bears deleterious effects to the host and is today considered as the major driver of inflammation and immunosuppression . - pubmed
INTRODUCTION : Ertugliflozin is an oral sodium - glucose cotransporter 2 inhibitor that is being developed to treat type 2 diabetes mellitus ( T2DM ) . - pubmed
At week 26 , both ertugliflozin / sitagliptin treatments provided significant reductions from baseline in HbA1c compared with placebo [ least squares mean HbA1c change ( 95 % confidence intervals ) from baseline was - 0.4 % ( - 0.7 , - 0.2 ) , - 1.6 % ( - 1.8 , - 1.4 ) , and - 1.7 % ( - 1.9 , - 1.5 ) for placebo , E5 / S100 , and E15 / S100 , respectively ] . - pubmed
Significant reductions in fasting plasma glucose , 2 - h post - prandial glucose , body weight , and systolic blood pressure were observed with both ertugliflozin / sitagliptin groups compared with placebo . - pubmed
Regarding the cell attachment , cells cultured on BaGs had enhanced integrinbeta1 and vinculin production , and mature focal adhesions were smaller but more dispersed than on cell culture plastic ( polystyrene ) . - pubmed
Moreover , the BaG - stimulated osteoinduction was significantly reduced by FAK and mitogen - activated protein kinase ( MAPK ) inhibitors , indicating an important role for FAK and MAPKs in the BaG - induced early osteogenic commitment of hASCs . - pubmed
Discovery of a novel 2 -( 1H - pyrazolo [ 3,4 - b ] pyridin-1 - yl ) thiazole derivative as an EP1 receptor antagonist and in vivo studies in a bone fracture model . - pubmed
A high - throughput immunoassay technique that quantitates the expression of relevant proteins of glycolysis , glycogenolysis , pentose phosphate pathway , oxidative phosphorylation , pyruvate and fatty acid oxidation , tricarboxylic acid cycle and the antioxidant response in two large independent and retrospectively collected cohorts of PEO - sD , PEO - mD and MELAS patients revealed that despite the heterogeneity of the genetic alterations , the three MDs showed the same metabolic signatures in both cohorts of patients , which were highly divergent from those of healthy individuals . - pubmed
Finally , we found TCL1 to act , at least in part , via increasing phospho - ERK1 / 2 and decreasing phospho - P38 MAPK . - pubmed
Because the PPARgamma antagonist BADGE and PI3K / Akt inhibitors wortmannin and LY29004 inhibited the intracellular lipid accumulation by NA and NAG associated with adipogenesis , it was considered that NA and NAG showed the above - mentioned activities via the activation of PPARgamma as well as phosphorylation of the PI3K / Akt pathway . - pubmed
Plants subjected to Pb - stress showed various deteriorations in cell organelles including visible alterations in chloroplasts , malformations in plant cells , abnormalities in the mitochondrial system , inward invagination of cell walls , distortions in the plasma membrane , oversized vacuoles and irregular increase in plastoglobuli formation . - pubmed
The mRNA levels of IFN - gamma was examined in ( n = 23 ) polyomavirus BK infected and ( n = 23 ) non - infected kidney transplant patients in comparison with healthy controls ( n = 23 ) , using an in - house Real time PCR ( SYBR Green ) assay . - pubmed
NADPH oxidases are enzymes that have been reported to generate reactive oxygen species ( ROS ) in animals , plants and many multicellular fungi in response to environmental stresses . - pubmed
Additionally , the transcription of vvnoxa , vvrac1 , vvbema and vvcdc24 could be repressed when the intracellular ROS were eliminated by diphenyleneiodonium ( DPI ) chloride and reduced glutathione ( GSH ) treatments . - pubmed
METHODS : In vitro 2 - D and 3 - D assays , and intracellular calcium influx assays were utilized to measure tumourigenic activity in response to altered CANCG4 levels and calcium channel blockers . - pubmed
CACNG4 functions by closing the channel pore , inhibiting calcium influx , and altering calcium signaling events involving key survival and metastatic pathway genes ( AKT2 , HDAC3 , RASA1 and PKCzeta ) . - pubmed
Our findings suggest an underlying pathway for tumour growth and dissemination regulated by CACNG4 that is significant with respect to developing treatments that target these channels in tumours with aberrant calcium signaling . - pubmed
Glioblastoma Multiforme Stem Cell Cycle Arrest by Alkylaminophenol Through the Modulation of EGFR and CSC Signaling Pathways . - pubmed
THTMP strongly inhibited NSCC and in a subtle way for GSC in a time - dependent manner and inhibit the resistance variants better than that of temozolomide ( TMZ ) . - pubmed
THTMP arrest the CSC cell population at both G1 / S and G2 / M phase and induce ROS - mediated apoptosis . - pubmed
THTMP arrests GBM stem cell cycle through the modulation of EGFR and CSC signaling pathways . - pubmed
The coding sequences of lcTRAF2 , lcTRAF7 , and lcTRADD were 1488 , 2454 , and 744 nucleotides , and they encoded proteins of 495 , 344 , and 248 amino acids , respectively . - pubmed
The aim of the study was to make a comparative studies on the effectiveness of platelet rich plasma ( PRP ) and hyaluronic acid ( HA ) in intra - articular injections to the temporomandibular joints - in double blind studies application - based on the analysis of selected clinical parameters of functional efficiency and the mean value of joint 's pain intensity before and after management . - pubmed
Study group PRP was treated with intra - articular injection of platelet rich plasma and study group HA had injection with hyaluronic acid . - pubmed
Carboxylated nanocrystalline cellulose ( CNCC ) was prepared by oxidation degradation of microcrystalline cellulose ( MCC ) using ammonium peroxydisulfate and modified with Fe3O4 nanoparticles to form Fe3O4 - CNCC nanocomposite via simple refluxing process . - pubmed
The Fe3O4 - CNCC nanocomposite doped poly ( 3,4 - ethylenedioxythiophene ) ( PEDOT ) was successfully decorated on the glassy carbon electrode ( GCE ) by electrochemical deposition . - pubmed
The PEDOT / Fe3O4 - CNCC modified GCE with enlarged real electrochemical surface area was used to determine nitrite with high selectivity , sensitivity and outstanding reproducibility . - pubmed
Here , we synthesized gold nanoparticles coated with selected osmolytes ( proline , hydroxyproline , and glycine ) and examined their effect on temperature - induced amyloid - formation of insulin hormone . - pubmed
METHODS : Jagged ends of cfDNA were detected by incorporating unmethylated cytosines during a DNA end - repair process , followed by bisulfite sequencing . - pubmed
Previously , we showed that in vitro morphine treatment significantly elevates Cav-1 expression and causes neuroplasticity changes . - pubmed
In this study , we confirmed that chronic morphine exposure can significantly increase Cav-1 expression ( P < 0.05 ) and microtubule - associated protein ( MAP-2)-positive neuronal dendritic growth in the hippocampus . - pubmed
Moreover , the rewarding effect and dendritic growth in the HPC induced by chronic morphine exposure were significantly inhibited by hippocampal Cav-1 knockdown . - pubmed
Together , these data suggest that Cav-1 in the hippocampus plays an essential role in the neuroplasticity changes that underlie morphine addiction behaviors . - pubmed
We have investigated the effects of the drugs cytochalasin B and colchicine on the surface levels of the large , external , transformation - sensitive ( LETS ) glycoprotein . - pubmed
Colchicine neither removed LETS protein from the surface , nor inhibited its regeneration after removal by mild trypsinization . - pubmed
Cells treated with cytochalasin B , however , showed both a 2 - 3 - fold increase in the turnover rate of their surface LETS protein and a marked inhibition in its regeneration . - pubmed
In fact , in the presence of cytochalasin B , increased quantities of LETS protein were released into the medium . - pubmed
In consideration of the patient 's age a conservative therapeutic program was instituted with a definite response to I-131 therapy despite poor radioactive iodine ( RAI ) uptake by the tumor . - pubmed
Partial nucleotide sequence analysis of the cDNA revealed a deletion of 243 nucleotides coding for 81 amino acids in a portion of the immunoglobulin - like domains of the extracellular region of the receptor . - pubmed
Do analogues of gonadotrophin releasing hormone influence follicular fluid steroid levels , oocyte maturity and fertilization rates ? - pubmed
The inflammatory effects of enalaprilat and cilazaprilat were tested in an experimental model of ovalbumin - sensitised guinea - pigs . - pubmed
Enalaprilat , but not cilazaprilat , enhanced the ovalbumin - induced inflammatory skin responses . - pubmed
Enalaprilat , applied locally , but not cilazaprilat , enhanced the inflammatory reactions caused by intradermal injections of allergen and substance P. - pubmed
Our study strongly indicates that enalaprilat has pro - inflammatory properties , whereas the new long - acting ACE inhibitor cilazaprilat does not . - pubmed
An objective of the present experiment was to measure the post - hypoxic drinking response at intervals during a 71 - d exposure to an atmosphere containing 12 % oxygen in nitrogen to assess whether the response could be observed throughout this entire period . - pubmed
However , the results of these studies indicate that administration of the betra - adrenoceptor antagonist , propranolol ( 6 mg / kg , i.p . ) - pubmed
These patients showed a more severe thalassaemia intermedia like clinical phenotype as compared to those with the same beta - globin genotype and a normal alpha - globin gene arrangement . - pubmed
A group of patients with thalassaemia intermedia from Southern Italy , either homozygous or heterozygous for haplotype I and in whom previous studies had failed to define the mutation in one of the beta thalassaemia globin genes , were screened by oligonucleotide analysis for the presence of the beta th-101 . - pubmed
Chlordecone has been reported to resemble estrogen in altering pituitary secretions , in producing vaginal cornification , in increasing uterine weight and in competing for binding to the estrogen receptor . - pubmed
In further studies , chlordecone 's effect on the CNS progesterone receptor was examined . - pubmed
Unlike estradiol , chlordecone did not induce progesterone receptors . - pubmed
Furthermore , chlordecone attenuated the increase in progesterone receptors seen after estradiol treatment . - pubmed
Pressure - volume curves were performed prior to and following the initiation of artificial pulmonary perfusion with 1 % bovine serum albumin and 5 % dextran in Tyrode 's solution . - pubmed
Morphine ( an agent inhibiting substance P release ) significantly attenuated the spasm , whereas the presence of substance P in the perfusate markedly enhanced the constriction . - pubmed
Depletion of endogenous substance P by chronic capsaicin pretreatment did not affect exogenous substance P - induced spasm . - pubmed
Effect of zinc ions on tRNA structure . - pubmed
RPC-5 contains dichlorodifluoroethylene beads coated with a quaternary ammonium compound where the substituents are : R1 = methyl , and R2 - 4 = C8 - 10 hydrocarbons . - pubmed
Aminex A-28 contains quaternary ammonium covalently attached to styrene - divinylbenzene copolymer lattice and R1 - 3 are methyl groups . - pubmed
The chromatography of tRNA in the presence and absence of Zn2 + is interpreted in terms of this model and the effects of sodium chloride concentration , temperature , and pH were explored as the experimental variables . - pubmed
These experiments suggest that in the absence of Zn2 + tRNA does not interact appreciably with the hydrophobic surface of the column . - pubmed
The addition of Zn2 + has three effects on chromatography : a decrease in the number of anionic sites on the tRNA which interact with the positively charged ammonium ion , an increase in affinity of the tRNA for these ionic sites , and an increase in affinity of tRNA for hydrophobic sites on the column . - pubmed
These results show that chromatography on RCP-5 can be a sensitive physical chemical technique for examination of the structure of tRNA , and probably for other nucleic acids as well . - pubmed
The tyrosine hydroxylase inhibitor alpha - methyltyrosine almost completely inhibited the accumulation of DOPA in all organs . - pubmed
The DOPA accumulation was enhanced in all organs by the alpha - adrenoceptor antagonists phentolamine and yohimbine . - pubmed
These results indicate ( a ) that sporozoites and merozoites share antigens recognized by serum IgG , ( b ) that there is no episode of marked immunosuppression during acute infection , and ( c ) that cellular immunity is probably more important in resistance against reinfection than humoral immunity . - pubmed
Such bronchoalveolar mast cells will release histamine in response to IgE - dependent challenge in a reaction that is dose- , time- and energy - dependent . - pubmed
In addition , bronchoalveolar mast cells from asthmatic subjects show an increased releasability of histamine in response to anti - IgE. - pubmed
Storage was evaluated by application of dual immunocytochemical stains with anti - LH beta and anti - FSH beta to cells stimulated with 0.5 nM [ D - Lys6 ] GnRH . - pubmed
GnRH had less striking effects on changing the size or number of LH and FSH plaques . - pubmed
In the basal state , there were 36.6 + /- 9 % LH and only 2.7 + /- 2 % FSH plaques , whereas after GnRH stimulation there were 43.1 + /- 10 % LH and 10.5 + /- 4 % FSH plaques . - pubmed
Tissue was double labeled for [ 3H]GABA uptake and glutamate decarboxylase ( GAD ) immunolocalization . - pubmed
Nearly 90 % of the amacrine cell synaptic processes onto both proximal and distal halves of mb1 terminals were labeled with either [ 3H]GABA or GAD - immunoreactivity ( IR ) . - pubmed
Proximal half : 73 % of the amacrine synapses were labeled with [ 3H]GABA uptake and 82 % with GAD - IR ; 88 % of [ 3H]GABA labeled contacts were double labeled . - pubmed
Distal half : 17 % of the amacrine synapses were labeled with [ 3H]GABA uptake and 67 % with GAD - IR ; 63 % of [ 3H]GABA labeled contacts were double labeled . - pubmed
[ The opiate sigma - agonist SKF 10.047 disturbs the early morphogenesis of the loach ] . - pubmed
The immunogen and enzyme marker were prepared by direct reaction of secalonic acid D with bovine serum albumin and horseradish peroxidase , respectively . - pubmed
[ Variations in the induced optic activity accompanying the association of certain dicarboxylic acids to aspartate aminotransferase ] . - pubmed
Four had positive findings simultaneously for hepatitis B surface antigen ( HBsAg ) and surface antibody , and five had positive findings for HBsAg alone . - pubmed
Certain cyclic compounds with a butyrolactone , tetrahydrofuran , furan , tetrahydropyran , or 2 - pyron ring showed substantial rescue activity in lon and ( or ) ruv mutants . - pubmed
The data imply that intracollicular mechanisms activated by NMDA and non - NMDA receptors contribute to the generation of the inhibitory responses in SCC which lead to response habituation . - pubmed
The homology to AAV2 was also confirmed at the amino acid level ( nonstructural protein 55 % , capsid coding region 51 % ) . - pubmed
Northern analysis indicates that a 2.7 - kb unc-7 message is present in all developmental stages but is most abundant in L1 - L3 larvae . - pubmed
We determined the sequence of a cDNA corresponding to the unc-7 message . - pubmed
The message may encode a 60 - kd protein whose amino acid sequence is unrelated to any other available protein sequence ; a transmembrane location for the unc-7 protein is predicted . - pubmed
We speculate that unc-7 may be involved in the function of neuronal ion channels . - pubmed
The circumvention of P - glycoprotein function by pharmacologic agents is a major focus of clinical trials aimed at increasing the cytotoxicity of multidrug resistance - associated drugs , including doxorubicin . - pubmed
UTP , alpha , beta - Me - ATP and ATP ( in the presence of an A1 purinoceptor antagonist ) produced a depolarisation in that order of potency . - pubmed
Of these , 32 tRNA species were identified by aminoacylation as tRNA specific for 15 amino acids . - pubmed
3 - Aminobenzamide ( 3 - ABA ) , a specific inhibitor of poly ( ADP - ribosyl ) polymerase ( PARP ) , a nuclear enzyme which is activated by breaks in DNA to catabolize intracellular NAD , is capable of relieving such a drop . - pubmed
At 20 h , when the apoptotic ladder - like pattern of DNA is visible , NAD concentration has dropped again , probably because of a second PARP activation due to the extensive DNA degradation that accompanies apoptosis . - pubmed
The presence of 3 - ABA , concomitantly with the preservation of the intracellular NAD content , reduces the extent of apoptosis upon oxidative stress and strongly enhances cell survival , thus suggesting a role for PARP in triggering stress - induced apoptosis . - pubmed
Moreover , in these instances the inhibition of PARP , although effective in blocking the drop in NAD concentration , has no effect on apoptosis , thus being only circumstantial . - pubmed
The LPSR has a comparatively high threshold of activation , its rate of development is inversely related to magnesium concentration and it can be eliminated by NMDA antagonists , as can the induction of PP . - pubmed
Quinazoline antifolates inhibiting thymidylate synthase : synthesis of gamma - linked peptide and amide analogues of 2 - desamino-2 - methyl - N10 - propargyl - 5,8 - dideazafolic acid ( ICI 198583 ) . - pubmed
Sensitive northern blot hybridization using digoxigenin RNA probes for the mRNA detection of two glucose transporter isoforms . - pubmed
This work shows that DIG - labelled glucose transporter ( GLUT1 and GLUT4 ) cRNAs are suitable probes for the assessment of these gene expressions . - pubmed
We have found that the DIG system offers a much higher sensitivity than the 32P system for both GLUT1 and GLUT4 mRNA detection . - pubmed
In addition , stability , safety , time saving and cost reduction are other considerations that make DIG - labelled GLUT1 and GLUT4 cRNAs very attractive . - pubmed
We have solved the 2.0 - A resolution crystal structures of four cavity - creating Ile / Leu-->Ala mutations in the hydrophobic core of barnase and compare and contrast the structural responses to mutation with those found for Leu-->Ala mutations in T4 lysozyme . - pubmed
Second , although it is currently accepted that hydrophobic cavities formed on the mutation of large hydrophobic side chains to smaller ones are not occupied by water molecules , we found a buried water molecule in the crystal structure of the barnase mutant Ile76 - ->Ala . - pubmed
Notably , the hydrogen bond between the cavity - water and the carbonyl group of Phe7 is also a marked early feature of very recent molecular dynamics simulations of barnase denaturation [ Caflisch , A. , & amp ; Karplus , M. ( 1995 ) J. Mol . - pubmed
It is possible that cavities engineered into the hydrophobic cores of other proteins may contain water molecules , even though they can not be detected by crystallographic analysis . - pubmed
Using the monoclonal MIB-1 antibody , which detects Ki-67 antigen in formalin - fixed , paraffin - embedded tissue , immunohistochemical studies were performed . - pubmed
Luteal phase progesterone profiles were assessed between the days LH surge +2 and LH surge +6 by means of a progesterone index . - pubmed
Similarly , no relation was established between LH and the quantitative assessment of luteal phase progesterone profiles ( progesterone index ) , and the distribution of progesterone indices was similar in the normal LH and elevated LH groups . - pubmed
Testosterone concentrations were positively correlated with LH ( p = 0.008 ) but not with the incidence of spontaneous follicular growth . - pubmed
CONCLUSION : The data indicate that both LH and testosterone secretion in PCOS were closely linked , but that neither was directly linked to the incidence or inhibition of spontaneous follicular development in PCOS , or to the disturbance in luteal phase progesterone profiles . - pubmed
In particular , the aromatase - immunoreactive cells of the lateral POM appear to be a key target for steroids in the activation of male copulatory behavior . - pubmed
The perfusion medium consisted of 95 % O2 - 5 % CO2 Krebs - Henseleit buffer , pH 7.4 , containing 5 g/100 ml bovine serum albumin , 100 microU / ml insulin , 5 mmol / l glucose , amino acids , and added fatty acids ( FA ) , including 0.1 microCi / ml [ 3H ] palmitate . - pubmed
Thus the relatively high TG synthesis rates observed in the high - oxidative muscle sections are not uniquely related to their high - flow capacities but are inherent to the TG synthesis process , probably events associated with FA uptake and/or capacity of the TG synthesis pathway . - pubmed
Similarly , InsP6 ( 100 microM , 30 s ) had no effect on basal superoxide anion generation and , compared to lipopolysaccharide ( LPS , 100 ng ml-1 , 60 min ) , tumour necrosis factor - alpha ( TNF alpha , 200 u ml-1 , 30 min ) or platelet - activating factor ( PAF , 100 nM , 5 min ) caused only a small enhancement of 100 nM fMLP - stimulated superoxide anion generation ( fold - increase in superoxide anion generation over fMLP alone : InsP6 1.8 + /- 0.3 , LPS 6.8 + /- 0.6 , TNF alpha 5.2 + /- 0.7 , PAF 5.8 + /- 0.6 ) . - pubmed
The authors examined therefore the thyroid function ( thyroid stimulating hormone , thyroxine , triiodothyronine , antibodies against thyroid peroxidase and against thyroglobulin ) in a random sample of 76 women after ablation of the breast on account of carcinoma . - pubmed
With a coding sequence of 315 nucleotides ( nt ) , corresponding to a protein of 104 amino acids , the SpliMNPV p10 gene is the longest p10 gene known . - pubmed
Upstream of the p10 sequences , an ORF of 552 bp was identified that potentially encodes a 184 amino acid protein of Mr 20925 , which showed 52.2 % identity with the encoded product of the SeMNPV xb187 gene . - pubmed
Immunologic effects of progesterone are mediated by a protein named the progesterone - induced blocking factor ( PIBF ) . - pubmed
The prolonged and excessive consumption of alcohol has been shown to predispose the host to a variety of infectious complications , which may be due , in part , to the inability to produce important activating and chemotactic cytokines . - pubmed
These findings indicate that ethanol feeding results in diminished production of chemotactic and/or activating cytokines from AMs ex vivo that may contribute to the impairment in lung inflammatory responses and antimicrobial host defense that is observed in the setting of alcohol ingestion / intoxication clinically and experimentally . - pubmed
HTLV - II - infected subjects had on average , an 11 % decrease in creatine kinase ( p = .006 ) , a minor increase in mean corpuscular volume ( p = .01 ) , and a slightly lower serum calcium level ( p = .0005 ) . - pubmed
It augmented insulin release from islets incubated for 90 min in the absence or presence of D - glucose ( 2.8 to 16.7 mM ) , this coinciding with stimulation of 45Ca net uptake . - pubmed
In perifused islets deprived of extracellular D - glucose for 45 min , BTS 67 582 ( 0.1 mM ) decreased 86Rb outflow from prelabelled islets , but failed to increase 45Ca efflux and insulin release . - pubmed
In the presence of D - glucose ( 7.0 mM ) , BTS 67 582 , whilst failing to decrease 86Rb + outflow , provoked rapid , sustained and rapidly reversible increases of both 45Ca2 + efflux and insulin output . - pubmed
BTS 67 582 ( 0.1 mM ) suppressed the inhibitory action of diazoxide ( 0.25 mM ) upon glucose - stimulated insulin release , but nevertheless augmented insulin output from islets incubated in the presence of 90 mM K + . - pubmed
All five mutations cause high spontaneous tyrosine phosphorylation of KIT . - pubmed
Instability of Taylor - Couette flow of helium II . - pubmed
The two receptors are tyrosine kinases and have an extracellular domain containing seven immunoglobulin - like loops and a split tyrosine - kinase domain . - pubmed
METHODS : A synthetic tyrosine phosphopeptide was used to raise an antibody that recognizes the phosphorylation state of tyrosine 1054/1059 in the KDR receptor . - pubmed
The IC50 values of sufentanil binding with and without fluvoxamine addition are 0.56+/-0.17 nM and 0.3+/-0.15 nM , respectively , indicating no direct effect on the occupancy of sufentanil on the mu - receptor by this serotonin reuptake inhibitor . - pubmed
HS - SPME coupled with GC - MS and HPLC techniques were employed to study the evolution of the volatile compounds and the individual anthocyanins , respectively . - pubmed
A method for the determination of underivatized amino acids based on capillary electrophoresis coupled to electrospray ionization mass spectrometry ( CE - ESI - MS ) is described . - pubmed
Under the optimized conditions , 19 free amino acids normally found in proteins and several physiological amino acids were well determined in less than 17 min . - pubmed
OBJECTIVES : To investigate whether the same dipolar model could explain the scalp CO ( 2 ) laser evoked potential ( LEP ) distribution after either hand or foot skin stimulation . - pubmed
Phototoxicity was quantified at various periods after exposure by fluorescence staining of the nuclei of membrane - compromised cells , by TdT - dUTP terminal nick - end labeling ( TUNEL ) of apoptotic cells and by Annexin V labeling for phosphatidylserine exposure . - pubmed
Intrathecal pretreatment with protein kinase C inhibitor calphostin C ( 100 and 250 ng ) attenuated the decrease of the paw withdrawal latency evoked by sciatic nerve ligation . - pubmed
The transport of iodide across the cell membrane is mediated by the sodium iodide symporter ( hNIS ) . - pubmed
Therefore , the transduction of the hNIS gene per se is sufficient to induce 125I (- ) transport in Morris hepatoma cells in vitro and in vivo . - pubmed
For a therapeutic application of the hNIS gene , however , additional conditions need to be defined which inhibit the iodide efflux . - pubmed
PDMP and phospholipase A2 inhibitors also blocked it . - pubmed
However , clofibrate was distinguished from BFA in that BFA action was insensitive to phospholipase A2 inhibitors and from NDGA in that NDGA stabilized microtubules against nocodazole and its action was almost insensitive to taxol . - pubmed
GDF-15 is a potent trophic factor for developing and 6 - OHDA - lesioned midbrain dopaminergic neurons in vitro and in vivo , matching the potency of GDNF . - pubmed
The released VEGF was 165 amino acid residues in either condition . - pubmed
The enzyme activity was measured in a reaction mixture containing either vehicle or calcium chloride ( 1 - 20 microM ) in the absence or presence of regucalcin ( 0.1 , 0.25 , or 0.5 microM ) . - pubmed
NO synthase activity was significantly increased by the addition of calcium ( 5 - 20 microM ) . - pubmed
This increase was completely abolished in the presence of trifluoperazine ( TFP ; 10 - 50 microM ) , an antagonist of Ca ( 2 +)/ calmodulin . - pubmed
The addition of regucalcin ( 0.1 - 0.5 microM ) caused a significant fall in the calcium - increased enzyme activity . - pubmed
The effect of regucalcin ( 0.25 microM ) in decreasing NO synthase activity was seen in the presence of ethylene glycol bis -( 2 - aminoethylether ) N , N , N ' , N '- tetraacetic acid ( EGTA , 1 mM ) or TFP ( 20 microM ) , indicating that regucalcin acts independent on Ca ( 2 +)/ calmodulin . - pubmed
The high - loaded ( 48 - 60 wt.% ) 2 - 4 nm tetragonal ZrO2 phase inserted in mesostructured silica SBA-15 by chemical solution decomposition ( CSD ) of Zr ( n - PrO ) 4 and activated at 873 K displayed approximately 3 times higher capacity for surface sulfate ions and , respectively , 1.5 - 2.2 times higher catalytic activity per gram of SO4 - ZrO2 / SBA-15 composite in condensation of MeOH with t - BuOH and dehydration of isopropanol compared with the regular bulk sulfated zirconia material . - pubmed
This screen identified a catalytic triad ( Ser82 , His235 , and Asp207 ) with a configuration similar to that of the catalytic triad of hydrolases . - pubmed
Analysis of BioH with a panel of hydrolase assays revealed a carboxylesterase activity with a preference for short acyl chain substrates . - pubmed
[ Comparative content of amino acids in the casein of the milk of certain animal species ] . - pubmed
Its efficacy appears to be related to induction of glutathione S - transferase enzymes , inhibition of prostaglandin E ( 2 ) ( PGE ( 2 ) ) production , or suppression of oxidative DNA adduct ( M(1)G ) formation . - pubmed
A daily oral dose of 3.6 g of curcumin is advocated for Phase II evaluation in the prevention or treatment of cancers outside the gastrointestinal tract . - pubmed
Additionally , serum LH and progesterone concentrations were studied to investigate whether premature luteinization was present in these cycles and whether it might be related to coasting duration . - pubmed
Effect of ionic strength on the organization and dynamics of tryptophan residues in erythroid spectrin : a fluorescence approach . - pubmed
In this article , we have used a combination of fluorescence spectroscopic approaches that include red edge excitation shift ( REES ) , fluorescence quenching , time - resolved fluorescence measurements , and chemical modification of the spectrin tryptophans to assess the environment and dynamics of tryptophan residues of spectrin under different ionic strength conditions . - pubmed
Our results show that while REES , fluorescence anisotropy , lifetime , and chemical modification of spectrin tryptophans remain unaltered in low and high ionic strength conditions , quenching of tryptophan fluorescence by the aqueous quencher acrylamide ( but not the hydrophobic quencher trichloroethanol ) and resonance energy transfer to a dansyl - labeled fatty acid show differences in tryptophan environment . - pubmed
Surprisingly , however , a combination of Phe-781 and Gly-604 could restore about 44 % of the SSIIa activity provided that Val-737 was conserved . - pubmed
Choline is formed as a result of the catalytic hydrolysis ( depending on the cholinesterase activity ) of the substrate acetylcholine . - pubmed
tRNA - guanine transglycosylase ( TGT ) is a key enzyme involved in the post - transcriptional modification of certain tRNAs in their anticodon wobble positions with queuine . - pubmed
To maintain the correct Watson - Crick base pairing properties of the wobble base ( and hence proper translation of the genetic code ) , TGT must recognize its heterocyclic substrate with high specificity . - pubmed
Herein , we report further studies probing the differential heterocyclic substrate recognition properties of the aspartate 143 mutant TGTs . - pubmed
This confirms the key role of aspartate 143 in maintaining the anticodon identities of the queuine - containing tRNAs and suggests that TGT mutants could be developed that would alter the tRNA wobble base base pairing properties . - pubmed
Photosynthetic assimilation of carbon dioxide and acetate by isolated chloroplasts . - pubmed
They seem to be involved in aromatase expression , generation of estrogens and activation of estrogen receptor alpha ( ERalpha ) in malignant breast epithelium . - pubmed
Namely , it increased activation of estrogen receptors , therefore , it was suspected to reduce or even overcome the inhibitory effect of tamoxifen on breast cell proliferation . - pubmed
Enzyme conformational dynamics during catalysis and in the ' resting state ' monitored by hydrogen / deuterium exchange mass spectrometry . - pubmed
This work reports the use of electrospray mass spectrometry for studying the conformational dynamics of enzymes by amide hydrogen / deuterium exchange ( HDX ) measurements . - pubmed
Experiments carried out on carboxypeptidase B in the absence of substrate and in the presence of saturating concentrations of hippuryl - Arg result in HDX kinetics that are indistinguishable . - pubmed
Immobility and lack of exercise , increased levels of proinflammatory cytokines , increased production of oxygen free radicals or impaired detoxification , low anabolic hormone output , malnutrition and reduced neurological drive have been advocated as being responsible for sarcopenia . - pubmed
It is reasonable to argue that the activity of these nodes results from the net balance of regulating mechanisms , as in the case of the GH / IGF-1 axis , the testosterone and cortisol functions , the pro- and anti - inflammatory cytokines and receptors . - pubmed
Rocuronium is an alternative to mivacurium for short procedures , without the risk of unexpected prolonged relaxation due to a possible defect in plasma cholinesterase . - pubmed
MEASURES OF OUTCOME : Endpoints were serum lipid levels of total cholesterol , HDL - C , LDL - C , VLDL - C , triglycerides and apolipoproteins A-1 and B-100 . - pubmed
No statistically significant differences were seen for total cholesterol , triglycerides , LDL - C , VLDL - C , apolipoprotein A-1 or apolipoprotein B-100 . - pubmed
Serum total cholesterol concentrations , low - density lipoprotein cholesterol concentrations , and liver function parameters decreased in the soy - based group and were significantly different from measurements in the traditional group ( P & lt ; 0.05 ) . - pubmed
No significant change in serum triacylglycerol levels , serum high - density lipoprotein cholesterol levels , and fasting glucose levels was found in the soy or traditional group . - pubmed
CONCLUSION : Soy - based low - calorie diets significantly decreased serum total cholesterol and low - density lipoprotein cholesterol concentrations and had a greater effect on reducing body fat percentage than traditional low - calorie diets . - pubmed
Calcium is involved in formation of high molecular weight adiponectin . - pubmed
CONCLUSION : These data indicate that calcium promotes the formation of HMW adiponectin , and calcium sequestration decreases HMW adiponectin . - pubmed
Gain of interaction of ALS - linked G93A superoxide dismutase with cytosolic malate dehydrogenase . - pubmed
Protein interactions of the Amyotrophic Lateral Sclerosis ( ALS)-linked copper - zinc superoxide dismutase ( hSOD1 ) G93A mutation were studied using a fluorescence resonance energy transfer ( FRET ) based screening system . - pubmed
The FRET results confirmed by pull - down immunoprecipitation indicated " gain - of - interaction " of the G93A - hSOD1 mutant with cytosolic malate dehydrogenase ( cytMDH)-a key enzyme in the malate - aspartate shuttle which is vital to neurons . - pubmed
Consistent with inhibition of the malate - aspartate shuttle , G93A - hSOD1 had lower malate and higher lactate levels compared to non - induced or Wild - Type - hSOD1 expressing cells . - pubmed
Complex functions of phosphatidylinositol 4,5 - bisphosphate in regulation of TRPC5 cation channels . - pubmed
The canonical transient receptor potential ( TRPC ) proteins have been recognized as key players in calcium entry pathways activated through phospholipase - C - coupled receptors . - pubmed
While it is clearly demonstrated that members of the TRPC3 / 6 / 7 subfamily are activated by diacylglycerol , the mechanism by which phospholipase C activates members of the TRPC1 / 4 / 5 subfamily remains a mystery . - pubmed
In this paper , we provide evidence for both negative and positive modulatory roles for membrane polyphosphoinositides in the regulation of TRPC5 channels . - pubmed
Depletion of polyphosphatidylinositol 4 - phosphate and phosphatidylinositol 4,5 - bisphosphate ( PIP2 ) through inhibition of phosphatidylinositol 4 - kinase activates calcium entry and membrane currents in TRPC5 - expressing but not in TRPC3- or TRPC7 - expressing cells . - pubmed
Inclusion of polyphosphatidylinositol 4 - phosphate or PIP2 , but not phosphatidylinositol 3,4,5 - trisphosphate , in the patch pipette inhibited TRPC5 currents . - pubmed
Paradoxically , depletion of PIP2 with a directed 5 - phosphatase strategy inhibited TRPC5 . - pubmed
Furthermore , when the activity of single TRPC5 channels was examined in excised patches , the channels were robustly activated by PIP2 . - pubmed
These findings indicate complex functions for regulation of TRPC5 by PIP2 , and we propose that membrane polyphosphoinositides may have at least two distinct functions in regulating TRPC5 channel activity . - pubmed
RCA - silenced plants were impaired in their attack - elicited jasmonate ( JA)-isoleucine ( Ile)/leucine levels , but RuBPCase - silenced plants were not , a deficiency that could not be restored by supplementation with Ile or attributed to lower transcript levels of JAR4 / 6 , the key enzyme for JA - Ile conjugation . - pubmed
From these results , we infer that JA - Ile / leucine signaling and the herbivore resistance traits elicited by JA - Ile are influenced by adenylate charge , or more generally , carbon availability in RCA- but not RuBPCase - silenced plants . - pubmed
Oligomerization of glycosyl - phosphatidylinositol - anchored proteins ( GPI - APs ) into high molecular weight complexes is an essential step for their apical sorting in polarized epithelial cells . - pubmed
Interestingly , when cells are depleted of cholesterol and treated with tunicamycin , treatments that by themselves do not affect PLAP sorting , PLAP is not able to oligomerize and is missorted to the basolateral surface , thus supporting an indirect role of N - glycosylation , possibly mediated by a raft - associated glycosylated interactor . - pubmed
In addition , the need for exogenous insulin to maintain glucose control has been reduced . - pubmed
Proliferating cell nuclear antigen ( PCNA ) and 5 - bromo-2 '- deoxyuridine ( BrdU ) labeling index in normal liver graft reached the highest on postoperative day ( POD ) 3 , whereas in fatty grafts the expression peak was on POD 7 . - pubmed
When compared with moderate MaS grafts B2 ( 70 % ROLT ) and mild MaS grafts A3 ( 60 % ) groups , the disparities of alanine aminotransferase and total bilirubin were significant . - pubmed
9 - ODA is captured in the antennal lymph and transported to the pheromone receptor(s ) in the sensory neuron membranes by pheromone binding proteins ( PBPs ) . - pubmed
A key issue is to understand how the pheromone , once tightly bound to its PBP , is released to activate the receptor . - pubmed
This dimerization is disrupted by a unique residue mutation since Asp35 Asn and Asp35 Ala mutants remain monomeric at pH 7.0 , as does native ASP1 at pH 4.0 . - pubmed
Asp35 is conserved in only approximately 30 % of medium - chain PBPs and is replaced by other residues , such as Asn , Ala and Ser , among others , thus excluding that they may perform domain swapping . - pubmed
The amyloid - beta ( Abeta ) peptide , the 39 to 43 amino acid peptide that plays a substantial role in Alzheimer ' s disease , has been shown to interact strongly with lipids both in vitro and in vivo . - pubmed
Following the reaction with the ICAT reagent , we applied one - dimensional gel electrophoresis with in - gel trypsin digestion of proteins and subsequent MALDI - TOF - TOF mass spectrometric techniques for identification of peptides . - pubmed
[ Preparation of monolithic polylaurylmethacrylate column and its application in capillary electrochromatographic separation of myoglobin digests ] . - pubmed
Association of CD69 up - regulation on CD4 + Cla+ T cells versus patch test , strip patch test and clinical history in nickel sensitization . - pubmed
CONCLUSIONS : CD69 up - regulation on CD4 + CLA+ T cells in vitro could not predict neither a positive PT or SPT result nor a positive clinical history to nickel sulfate . - pubmed
A novel assay employing D - enantiomers of phospholipids as diluents for characterizing surface kinetics of lipid hydrolysis by phospholipases is introduced . - pubmed
Activity data show excellent agreement with a kinetic model derived with D - enantiomers as diluents and also that D - phospholipids bind to the enzyme but resist hydrolysis ; underscoring rationale ( i ) . - pubmed
Computational and biological analyses of the IL-27 binding site 1 to its receptor revealed important structural proximities with the ciliary neurotrophic factor group of cytokines and highlighted the contribution of p28 Trp ( 97 ) , as well as of EBI3 Phe(97 ) , Asp(210 ) , and Glu(159 ) , as key residues in the interactions between both cytokine subunits . - pubmed
A study of IL-27 binding site 3 showed that Trp(197 ) was crucial for the cytokine 's interaction with gp130 , but that the mutated cytokine still recognized IL-27R on the cell surface . - pubmed
BACKGROUND , AIM , AND SCOPE : Methacrylates are alpha , beta - unsaturated esters that are widely used in the polymer plastics and resins production . - pubmed
Methylpyruvate , ethylpyruvate , and butylpyruvate / butanol were identified as main degradation products respectively in the GC - MS analysis . - pubmed
The cardiac output ( Q(c ) , acetylene rebreathing ) response to exercise was determined from linear regression of Q(c ) and VO ( 2 ) ( Douglas bags ) at rest , ~30 % and ~60 % of peak VO(2 ) , and maximal exercise . - pubmed
Tamoxifen , an antiestrogen used in the prevention and treatment of breast cancer , is extensively metabolized by cytochrome P450 enzymes . - pubmed
Its biotransformation to alpha - hydroxytamoxifen ( alpha - OHT ) , which may be genotoxic , and to N - desmethyltamoxifen ( N - DMT ) , which is partially hydroxylated to 4 - hydroxy - N - DMT ( endoxifen ) , a potent antiestrogen , is mediated by CYP3A enzymes . - pubmed
We observed that the catalytic efficiency [ intrinsic clearance ( CL(int ) ) ] for alpha - OHT formation with recombinant CYP3A4 was 5 - fold higher than that with recombinant CYP3A5 ( 0.81 versus 0.16 nl min-1 pmol cytochrome P450 - 1 ) . - pubmed
For N - DMT formation , the CL(int ) with recombinant CYP3A4 was only 1.7 - fold higher , relative to that with recombinant CYP3A5 . - pubmed
In addition , the CL(int ) for N - DMT formation by HLM with CYP3A5 * 3 /* 3 alleles was approximately 3 - fold lower than that for HLM expressing CYP3A5 * 1 /* 1 . - pubmed
Regression analyses of tamoxifen metabolism with respect to testosterone 6beta - hydroxylation facilitated assessment of CYP3A5 contributions to the formation of the two metabolites . - pubmed
The CYP3A5 contributions to alpha - OHT formation were negligible , whereas the contributions to N - DMT formation ranged from 51 to 61 % . - pubmed
Our findings suggest that polymorphic CYP3A5 expression may affect the formation of N - DMT but not that of alpha - OHT . - pubmed
The structures of the separated compounds were identified by EI - MS , ( 1 ) HNMR and ( 13 ) CNMR . - pubmed
Here , we show that suppression of network activity with tetrodotoxin , which increases surface expression of AMPA receptors ( AMPARs ) , dramatically reduces levels of the deSUMOylating ( where SUMO is small ubiquitin - like modifier ) enzyme SENP1 , leading to a consequent increase in protein SUMOylation . - pubmed
Overexpression of the catalytic domain of SENP1 prevents this scaling effect , and we identify Arc as a SUMO substrate involved in the tetrodotoxin - induced increase in AMPAR surface expression . - pubmed
Synthesis and biological evaluation of thiophene derivatives as acetylcholinesterase inhibitors . - pubmed
The acetylcholinesterase inhibition activity was assayed according to Ellman 's method using donepezil as reference . - pubmed
Mechanisms of Cisplatin - Induced Apoptosis and of Cisplatin Sensitivity : Potential of BIN1 to Act as a Potent Predictor of Cisplatin Sensitivity in Gastric Cancer Treatment . - pubmed
Cisplatin forms inter- and intrastrand crosslinked DNA adducts and its cytotoxicity is mediated by propagation of DNA damage recognition signals to downstream pathways involving ATR , p53 , p73 , and mitogen - activated protein kinases , ultimately resulting in apoptosis . - pubmed
In addition , a new mechanism has recently been revealed , in which the oncoprotein c - Myc suppresses bridging integrator 1 ( BIN1 ) , thereby releasing poly ( ADP - ribose ) polymerase 1 , which results in increased DNA repair activity and allows cancer cells to acquire cisplatin resistance . - pubmed
The present paper focuses on the molecular mechanisms of cisplatin - induced apoptosis and of cisplatin resistance , in particular on the involvement of BIN1 in the maintenance of cisplatin sensitivity . - pubmed
After culture for 1 , 12 , 24 and 48 h following IPL irradiation , fibroblasts and the vascular endothelial cell line were harvested for investigation of morphological changes by light microscopy , cell proliferation viability by MTT assay , and VEGF and MMP secretions by ELISA . - pubmed
AIM : To compare the effects of telbivudine ( LDT ) and entecavir ( ETV ) in treatment of hepatitis B e antigen ( HBeAg)-positive chronic hepatitis B by meta - analysis . - pubmed
And the rate of increased creatine kinase in the LDT group was higher than in the ETV group ( RR 5.58 , 95 % CI 2.22 , 13.98 , P = 0.0002 ) . - pubmed
The activity of AtNUDX26 required Mg2 + or Mn2 + ions as cofactor and was optimal at pH 9.0 and 50 C. The expression of AtNUDX26 and of ppGpp metabolism - associated genes was regulated by various types of stress , suggesting that AtNUDX26 regulates cellular ppGpp levels in response to stress and impacts gene expression in chloroplasts . - pubmed
Dual phosphoinositide 3 - kinase / mammalian target of rapamycin inhibitor NVP - BEZ235 has a therapeutic potential and sensitizes cisplatin in nasopharyngeal carcinoma . - pubmed
To ascertain a therapeutical approach of nasopharyngeal carcinoma ( NPC ) , we hypothesized NVP - BEZ235 , a novel and potent imidazo [ 4,5 - c ] quinolone derivative , that dually inhibits both PI3 K and mTOR kinases activities , had antitumor activity in NPC . - pubmed
In NPC cell lines , with the extended exposure , NVP - BEZ235 selectively inhibited proliferation of NPC cells harboring PIK3CA mutation , compared to cells with wild - type PIK3CA . - pubmed
Furthermore , exposure of NPC cells to NVP - BEZ235 resulted in G1 growth arrest by Propidium iodide uptake assay , reduction of cyclin D1and CDK4 , and increased levels of P27 and P21 by Western blotting , but negligible apoptosis . - pubmed
Moreover , we found that cisplatin ( CDDP ) activated PI3K / AKT and mTORC1 pathways and NVP - BEZ235 alleviated the activation by CDDP through dually targeting PI3 K and mTOR kinases . - pubmed
In combination with NVP - BEZ235 and CDDP , there was dramatic synergy in shrinking tumor volumes and inducing apoptosis through increasing Noxa , Bax and decreasing Mcl-1 , Bcl-2 . - pubmed
Subclinical cardiovascular disease was expressed as cIMT , carotid arterial stiffness and secondarily as a metabolic risk z - score including the homoeostasis model assessment score of insulin resistance , triglycerides , total cholesterol to high - density lipoprotein ratio , inverse of cardiorespiratory fitness , systolic blood pressure and the sum of four skinfolds . - pubmed
His - tagged HP , incubated at pH 2.0 and 58 C , readily formed amyloid fibrils , as observed by thioflavin fluorescence , CD spectra , and transmission or atomic force microscopies . - pubmed
Amyloid fibril was also readily formed from full - length His - HP when incubated with 10 - 20 % 2,2,2 - trifluoroethanol at pH 7.8 and 25 C without peptide bond cleavage . - pubmed
Neutral sugar content showed higher values in SS ( 21.740 mg / mL ) and SL ( 18.657 mg / mL ) when compared with KS ( 1.497 mg / mL ) and KL ( 1.452 mg / mL ) ( p<0.05 ) . - pubmed
Total polyphenol contents of SL ( 423.139 mug / mL ) was significantly higher value ( p<0.05 ) than those of other samples , which occurred in the following order : SL> ; KL ( 218.494 mug / mL)> ; KS ( 107.269 mug / mL)> ; SS ( 75.894 mug / mL ) . - pubmed
KL exerted the highest DPPH radical scavenging activity ( 84.834 % ) ( p<0.05 ) , which occurred in the following order : KL> ; SL ( 63.473%)> ; KS ( 52.894%)> ; SS ( 35.443 % ) . - pubmed
ABTS radical scavenging activity showed that SL ( 66.088 % ) and KL ( 38.511 % ) had higher scavenging activities , whereas SS ( 7.695 % ) and KS ( 9.609 % ) demonstrated to be lower activities ( p<0.05 ) . - pubmed
Synthesis of ( 3 - hydroxy - pyrazolin-5 - yl ) glycine based ligands interacting with ionotropic glutamate receptors . - pubmed
These compounds were measured separately in the gas phase and the particle phase using a two - stage cartridge equipped with a quartz fiber filter ( 1 mum mesh ) and C18 solid - phase extraction disk for sampling and analyzed by GC / MS and LC / MS / MS ( for the detection of brominated flame retardants ) . - pubmed
Among the compounds detected in this study , those with lower molecular weights tended to be captured in the C18 solid - phase extraction disk rather than in the quartz fiber filter . - pubmed
Conditionally deleting Sox2 from the population of nascent cholinergic amacrine cells perturbed the normal ratio of cells situated in the GCL versus the INL and induced a bistratifying morphology , with dendrites distributed to both ON and OFF strata within the inner plexiform layer . - pubmed
A selective 5 - HT7 agonist , AS-19 , was totally ineffective in reducing S - IRA . - pubmed
Transcripts predicting 15 putative neuropeptide prepropeptides ( ( allatostatin , allatotropin , bursicon alpha , corticotropin releasing factor ( CRF ) , CRF - binding protein , eclosion hormone , FMRFamide , glycoprotein A , insulin - like peptide , ion transport peptide , myoinhibitory peptide , inotocin ( = neurophysin - oxytocin ) , Neuropeptide F , sulfakinin and SIFamide ) ) and transcripts predicting receptors for 14 neuropeptides ( allatostatin , calcitonin , cardioacceleratory peptide , corazonin , CRF , eclosion hormone , gonadotropin - releasing hormone / AKH - like , insulin - like peptide , neuropeptide F , proctolin , pyrokinin , SIFamide , sulfakinin and tachykinin ) are reported . - pubmed
Additionally , transcripts predicting 6 neurotransmitter / neuromodulator receptors ( acetylcholine , GABA , dopamine , glutamate , octopamine and serotonin ) and 3 neurotransmitter transporters ( GABA transporter , noradrenalin - norepinephrine transporter and Na +- neurotransmitter / symporter ) are described . - pubmed
RESULTS : SUA positively correlated with laboratory nutritional markers ( albumin , creatinine ) , body composition parameters , HGS ( r = 0.26 ; P & lt ; 0.001 ) and GNRI ( r = 0.34 ; P & lt ; 0.001 ) . - pubmed
ROW1 specifically binds trimethylated histone H3 lysine 4 ( H3K4me3 ) in the WOX5 promoter region to repress its transcription . - pubmed
Furthermore , the carboxyl - terminal domain determines myosin II localization in the constriction region of PPT and is responsible for the specific role of NMII in platelet release . - pubmed
This inhibition was suppressed by silencing IL-10R1 and was mimicked by cobalt Protoporphyrin IX and CO - releasing molecule-2 . - pubmed
Taken together , our results suggest that IL-10 / HMOX1 signaling is involved in modulation of cochlear inflammation through inhibition of MCP-1 / CCL2 regulation in SLFs , implying a therapeutic potential for a CO - based approach for inflammation - associated cochlear diseases . - pubmed
Regression analysis was performed to look for any association of low Mg0 with admission blood pressure ( BP ) and Glasgow Coma Scale ( GCS ) scores . - pubmed
Mg0 levels were negatively associated with systolic BP presentation ( P & lt ; 0.0001 ) and positively associated with the initial GCS scores ( P = 0.01 ) . - pubmed
Mg levels on admission correlate inversely with systolic BP and directly with GCS scores at presentation . - pubmed
Furthermore , cerebrovascular CO2 reactivity was assessed as a second metric of CBF regulation by inducing hypercapnia with 8 % CO2 inhalation followed by hyperventilation driven hypocapnia . - pubmed
The aim of this study was to confirm adjuvant chemotherapy of HSS with docetaxel plus cisplatin ( DP ) against NSCLC by evaluating antitumor activity and attenuated effect . - pubmed
The patient resumed proton pump inhibitor therapy ( esomeprazole ) without incident . - pubmed
The dietxtimexinsulin resistance status interaction was not statistically significant in the model for overall weight loss , although insulin sensitive women at 12months lost more weight in the lower fat vs. lower carbohydrate group ( 7.5 kg vs. 4.3 kg , P=0.06 ) , and in the walnut - rich vs. lower carbohydrate group ( 8.1 kg vs. 4.3 kg , P=0.04 ) . - pubmed
Sex hormone binding globulin increased within each group except in the lower carbohydrate group at 12months ( P<0.01 ) . - pubmed
Green cone opsin belongs to the G protein - coupled receptor superfamily and consists of a seven transmembrane helical apoprotein covalently bound to 11 - cis - retinal , by means of a protonated Schiff base linkage , in its inactive dark state . - pubmed
A double Cys mutant that could form a stabilizing disulfide bond was used in an attempt to address the instability of the green opsin mutants . - pubmed
Renal sodium handling and blood pressure changes in gestational protein - restricted offspring : Role of renal nerves and ganglia neurokinin expression . - pubmed
BACKGROUND : Considering long - term changes in renal sodium handling and blood pressure in maternal protein - restricted ( LP ) offspring , we assumed that the development of LP hypertension results from abnormal dorsal root ganglia ( DRG ) neurokinin expression associated with impaired responsiveness of renal sensory receptors , promoting a reduced urinary excretion of sodium . - pubmed
In addition , we assessed the tubular sodium handling , estimated by creatinine and lithium clearances before and after bilateral renal denervation in conscious LP offspring relative to age - matched NP counterparts . - pubmed
CONCLUSIONS : These observations raise the possibility that impaired DRG and pelvic neurokinin expression associated with responsiveness of renal sensory receptors in 16 - wk old LP offspring are conducive to excess renal reabsorption of sodium and development of hypertension in this programmed model . - pubmed
None of the deletion mutants induced apoptosis as efficiently as full - length RELT , a result that is consistent with a novel DD being located at the carboxyl - terminus . - pubmed
The inhibition of mevalonate kinase ( MVK ) by downstream metabolites is an important mechanism in the regulation of isoprenoid production in a broad range of organisms . - pubmed
mazei MVK , these enzymes were not inhibited by diphosphomevalonate ( DPM ) , dimethylallyl diphosphate ( DMAPP ) , isopentenyldiphosphate ( IPP ) , geranylpyrophosphate ( GPP ) or farnesylpyrophosphate ( FPP ) . - pubmed
However , they exhibited significantly higher affinity to mevalonate and higher catalytic efficiency than the previously characterized enzyme . - pubmed
Benzo [ c ][ 1,2,5 ] thiadiazole derivatives : A new class of potent Src homology-2 domain containing protein tyrosine phosphatase-2 ( SHP2 ) inhibitors . - pubmed
Using scaffold - hopping strategy , a series of benzo [ c ][ 1,2,5 ] thiadiazole derivatives was designed from PTP1B inhibitors with 1H-2,3 - Dihydroperimidine motif , synthesized and evaluated their biological activities against PTP1B and SHP2 . - pubmed
Characterization of a nicotinic acetylcholine receptor binding site for sulfoxaflor , a new sulfoximine insecticide for the control of sap - feeding insect pests . - pubmed
Through the evaluation of a set of SFX analogs , we have demonstrated that displacement of [ 3H]SFX shows an excellent correlation with GPA toxicity , and thus is toxicologically relevant . - pubmed
Comparison with the previously described methyl - SFX binding site information reveals differences with the SFX binding site that are discussed herein . - pubmed
In conclusion , CC homozygosis in the SNP - 174 G>C gene promoter of IL-6 can be proposed as one of the gene variants influencing iron accumulation in male adults with HFE mutations . - pubmed
RESULTS : The mean time taken to apply the DMF was 3.8 min ; for ICDAS , it took 8.9 min , and for CAST , 4.7 min . - pubmed
When calculating the indicator the most severe caries lesion per individual , the prevalence rates were as follows : 28.1 % for DMF , 84.0 % for ICDAS and 75.0 % for CAST . - pubmed
CAST described disease distributions very well and identified lesion severities and preventive and curative needs in the examined group , and the time required to apply CAST was similar to that of the DMF . - pubmed
Therefore , the analytical results of biochemical indicators , histopathological observations and western blotting showed that TLR and DTG exhibited a significant effect in renal protection . - pubmed
Furthermore , TLR and DTG prevent high glucose - induced glomerular mesangial cells ( GMCs ) by inhibiting TGF - beta1 and Wnt / beta - catenin signaling pathway , providing a powerful supports to develop a new therapeutic agent for DN . - pubmed
This finding shows that N - glycans attached to htau may affect its aggregation . - pubmed
This system allowed us to investigate the effects of antisense oligoribonucleotides representing functional domains of guinea pig IFN - Alpha1 AS on gpIFNA1 mRNA levels and , consequently , on viral proliferation in the respiratory tract of influenza virus - infected animals . - pubmed
Our study provides evidence that DSF inhibits Aldehyde dehydrogenase ( ALDH ) enzyme activity , inhibits the expression of stemness - related transcription factors ( Sox , Nanog , Oct ) in CSC derived from breast cancer cell lines , and modulates intracellular reactive oxygen species ( ROS ) generation . - pubmed
Importantly , our research proved that ALDH + stem - like cells play important roles in the resistance to the conventional chemotherapeutic agent cisplatin . - pubmed
DSF enhances the cytotoxic effect of cisplatin through inhibiting the stemness and by overcoming cisplatin resistance of ALDH + stem - like cells . - pubmed
Further , we show that ALDH + cancer stem - like cells and ALDH- bulk cancer cells have different intrinsic ROS levels , what may explain differences in susceptibility to cisplatin treatment . - pubmed
The results showed that UFL1 depletion aggravated the LPS - induced inflammatory response and cell damage by positively regulating the TLR4 / NF - kappaB pathway ( increased the expression of TLR4 , NF - kappaB P65 in nuclear , and phospho - IkappaBalpha ) , exacerbating LPS - induced ER stress ( increased the expression of CHOP , Hsp70 , and GRP78 ) , apoptosis ( increased the expression of Bax / Bcl-2 and activity of caspase-3 ) , autophagy ( increased LC3 - II and decreased P62 expression ) , and oxidative stress ( decreased SOD and CAT levels and increased MDA levels ) . - pubmed
Intracellular FAS activity was measured by a spectrophotometer at 340 nm of NADPH absorption . - pubmed
Palmitic acid , the end product of FAS catalyzed reaction , rescued the ER stress and autophagy induced by alpha - mangostin . - pubmed
To solve these problems , a smart albumin - based tumor microenvironment - responsive nanoagent is designed via the self - assembly of human serum albumin ( HSA ) , dc - IR825 ( a cyanine dye and a photothermal agent ) , and gambogic acid ( GA , a heat shock protein 90 ( HSP90 ) inhibitor and an anticancer agent ) to realize molecular targeting - mediated mild - temperature PTT . - pubmed
Here , we identify a pilin peptidase iamP , and LysR - type regulator ( LRTR ) iamR , that fulfil these roles and show that environmental calcium , which oysters enrich for shell repair and growth , regulates iam expression . - pubmed
PiamR exhibited classical negative auto - regulation but , strikingly , IamR inversely regulated the divergent iamP and iamA promoters in a calcium - dependent manner . - pubmed
Moreover , expression of the c - di - GMP and calcium - regulated , biofilm - promoting brp exopolysaccharide was IamA - dependent . - pubmed
These results support a scenario in which the calcium - enriched oyster environment triggers IamP - mediated processing of prepilin amassed in the periplasm for rapid pilin elaboration and subsequent BRP production to promote colonization . - pubmed
Conversely , the rhizosphere soil acid phosphatase ( RS - APase ) activity was significantly higher ( 26 - 46 % ) in the IC treatments and occurred concomitant with a significant increase in available phosphorus ( RS - Pavailable ) ( 26 - 74 % ) in the rhizosphere . - pubmed
In the present study , the network pharmacology method was used to construct the ingredient - target network of DHA that was responsible for the anti - HCC effect and 11 targets including ALB , ATP5A1 , CCT3 , CLIC1 , ENO1 , HSPA8 , HSPB1 , NPM1 , PPIA , PRDX1 , and ZYX were selected . - pubmed
Further lectin immunofluorescence and lectin blot analysis showed that DHA could down - regulate the expression of beta1,6 - branching of N - linked carbohydrate by suppressing the transcription of MGAT5 . - pubmed
The target genes and active ingredients of THH against RA comprised 64 genes and 17 compounds , the focus of which was PTGS2 , triptolide , and celastrol . - pubmed
SystemsDock website indicated that the combing degree of PTGS2 with triptolide or celastrol was very good . - pubmed
The mechanisms of THH against RA were linked to 31 signaling pathways , and the key mechanism was related to inhibition of inflammation response through inactivating TNF and NF - kappa B signaling pathways . - pubmed
Here , we report a route to access metal - diamidobenzoquinone frameworks from their dihydroxobenzoquinone counterparts via post - synthetic linker exchange . - pubmed
Combinatorial Roles of Mitochondria and cGMP / PKG Pathway in the Generation of Neuronal Free Zn2 + under the Presence of Nitric Oxide . - pubmed
The molecular mechanism of NZR has been extensively investigated , but the source of Zn is present of a major candidate that is yet to be answered . - pubmed
RESULTS : Two mechanisms , namely , mitochondria as the only Zn source and the opening of NO - PKG - dependent mitochondrial ATP - sensitive potassium channels ( mKATP ) as the key to releasing NO - induced increase in mitochondrial Zn , were proven to be the two critical paths of NZR in neuronal - related cells . - pubmed
The genes for the key enzymes of the pathway were disrupted by insertion mutagenesis and the cellular metabolites in the methanol extracts of mutant cells were analyzed by HPLC . - pubmed
The deletion of the doeA gene resulted in accumulation of the higher level of ectoine compared to the wild type strain . - pubmed
Ngamma - acetyl - L-2,4 - diaminobutyrate ( Ngamma - acetyl - DAB ) , a substrate for ectoine synthase , was found in the cytoplasm of the wild type strain . - pubmed
Nalpha - acetyl - L-2,4 - diaminobutyrate ( Nalpha - acetyl - DAB ) , a substrate for the DoeB enzyme , appeared in the cells as a result of exposure of the doeB mutant to low osmotic pressure . - pubmed
The genes for the enzymes involved in ectoine degradation were found in all aerobic methylotrophs capable of ectoine biosynthesis . - pubmed
We have shown that ectoine hydrolase DoeA in this methanotroph hydrolyzes ectoine with the formation of the only isomer : Nalpha - acetyl - DAB . - pubmed
Moreover , p - hydroxycinnamic acid - based Meldrum ' s derivatives possess valuable secondary activities sought by the cosmetic industry such as antioxidant and anti - tyrosinase properties . - pubmed
Following 12 months of supplementation with resveratrol versus placebo , there were positive effects on bone density in the lumbar spine ( +0.016 + -0.003 g / cm2 ) and neck of femur ( +0.005 + -0.002 g / cm2 ) , which were accompanied by a 7.24 % reduction in C - terminal telopeptide type-1 collagen levels , a bone resorption marker , compared to placebo . - pubmed
Presented results clearly show the influence of ZnO decomposition and segregation of Zn atoms during evaporation and post - deposition annealing on microstructure and optical properties of zinc oxide films . - pubmed
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening . - pubmed
Binding affinity and the capacity of the amido analogues to stimulate the phosphotransferase activity of the kinases is greatly reeuced relative to cAMP , the 3'-position being more sensitive towards the modification than the 5'-position . - pubmed
The coordinate noncyclic derivatives , 3 '- deoxy-3 '- amino-5 '- AMP ( IV ) and 5 '- deoxy-5 '- amino-3 '- amp ( iii ) , were also tested . - pubmed
Surprisingly activity towards protein kinases was found to be considerable for the 5 '- deoxy-5 '- amino-3 '- AMP ( III ) , while the 3 '- deoxy-3 '- amino-5 '- AMP ( IV ) is practically inactive . - pubmed
The phosphodiesterase splits both cyclic amido analogues but with reduced rates compared to that of natural cAMP . - pubmed
Gestational diabetes was no less severe ( degree of hyperglycaemia , need for insulin therapy ) when associated with norethisterone . - pubmed
Acetylcholine , nicotine and the neuronal nAChR agonist dimethylphenylpiperazinium iodide ( DMPP ) , but not muscarine , all evoked inward currents in the cells ( voltage - clamped at -60 mV ) . - pubmed
DMPP 's actions were concentration- and voltage - dependent , and were antagonised by the neuronal nAChR antagonist mecamylamine ( 1 - 3 microM ) . - pubmed
We have isolated from an HEL cell cDNA library an alternatively spliced transcript for the platelet membrane glycoprotein IIb ( GPIIb ) that resulted from the deletion of the 34 amino acids of exon 28 of the GPIIb gene . - pubmed
However , absence of the carbohydrate appended to exon 28 did not prevent normal GPIIb / IIIa heterodimer expression . - pubmed
Our studies indicate that absence of the amino acids encoded by GPIIb exon 28 sufficiently perturbs the quaternary configuration of the GPIIb / IIIa heterodimer to impair its subsequent intracellular transport and processing . - pubmed
to photolysis of caged ATP and hydrolysis of ATP , were separated from the absorbance changes due to the active ATPase reflecting formation of the phosphorylated Ca2E1P enzyme form . - pubmed
Transmural nerve stimulation ( TNS ) induced a frequency - dependent increase in perfusion pressure in all vessels that was blocked by tetrodotoxin , phentolamine , and prazosin . - pubmed
The inhibitory effect of endothelium was not modified by indomethacin but was abolished by hemoglobin , indicating that endothelium - derived relaxant factor ( EDRF ) was the vasodilator involved . - pubmed
The endothelium - dependent inhibitory effect ( rubbed vessel minus control vessel contractions ) increased with time during the first 10 - 20 s after the beginning of TNS , and was frequency dependent and inhibited by low doses of phentolamine , which suggest a receptor - mediated mechanism . - pubmed
Effects of the potassium channel blocking dendrotoxins on acetylcholine release and motor nerve terminal activity . - pubmed
Insulin had no effect on the capacity of erythrocytes to absorb glucose . - pubmed
Affinities to estrogen , progesterone , and androgen receptors , however , were very low . - pubmed
Quantification is performed by single ion monitoring of CAD species of oxytetracycline and of its internal standard . - pubmed
The effects of two disulphides on cholinesterase activity in the spectrophotometric assay . - pubmed
5,5 '- Dithiobis -( 2 - nitrobenzoate ) did not influence serum cholinesterase activity , whereas 2,2 '- dithiodipyridine had an inhibitory effect . - pubmed
Tightly bound oxalacetate and the activation of succinate dehydrogenase . - pubmed
Inhibition of tyrosine hydroxylase by pyrroloisoxazoles . - pubmed
Enalapril maleate is unlike the prototype angiotensin - converting enzyme inhibitor captopril in that a standard meal does not appear to influence absorption of this new drug . - pubmed
One of the siblings possessed only one of the relevant haplotypes , and was shown to be heterozygous for 21 - OH deficiency by the 17 alpha hydroxyprogesterone response to ACTH stimulation . - pubmed
When the cells were stimulated with PWM in the presence of a physiological concentration of hydrocortisone ( HC ) , the secretion of IgG was significantly higher in HLA - B8 - positive individuals . - pubmed
One type of aspiny neuron had an elongated , usually spindle - shaped , medium - size soma with two , or rarely three , primary dendrites originating from opposite poles of the cell ; one example of this type of neuron had two separate axons . - pubmed
The possibility of characterizing subgroups of depressive disorders by biological markers was studied by means of the dexamethasone suppression test ( DST ) , the 24 - hr urinary free cortisol ( UFC ) , the growth hormone response to the insulin tolerance test ( ITT ) , and polygraphic sleep recordings . - pubmed
The substrate binding site of the enzyme was found to consist of seven subsites , numbered -IV , -III , -II , -I , I , II and III , towards the subsite binding the reducing end unit of xyloheptaose . - pubmed
As a consequence of such an almost symmetric distribution of affinities around the catalytic groups , the enzyme cleaves preferentially the bonds in the oligosaccharides which are most distant from both terminals . - pubmed
A hypothesis is discussed according to which only type I cells are primarily responsive to PHA , whereas type II cells require " help " from the former , prior to transition into the proliferative stage . - pubmed
The transport activity for cystine was enhanced when the cells were incubated with tumour necrosis factor - alpha ( TNF - alpha ) , but not with interferon - gamma ( IFN - gamma ) or interleukin-1 . - pubmed
The IGF I receptor ( IGF IR ) is a heterotetramer composed of two cross - linked extracellular alpha - chains and two membrane - spanning beta - chains that contain a tyrosine - kinase domain . - pubmed
It has a high degree of sequence similarity to the insulin receptor ( IR ) , and the putative ligand - specific binding site has been localized to a cysteine - rich region ( CRR ) of the alpha - chain . - pubmed
A polyclonal antibody directed against a 48 - amino acid synthetic peptide , corresponding to a region of the CRR postulated to be essential for ligand binding , failed to react with either solubilized , reduced or intact IGF IR . - pubmed
The extracellular amino - terminal part , TM IV and the second extracellular loop contain several replacements suggesting that these portions have no or limited direct interactions with the peptide ligands . - pubmed
Effect of oxygen abstraction on the peroxidase - luminol - perborate system : relevance to the HRP enhanced chemiluminescence mechanism . - pubmed
Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated . - pubmed
It is , therefore , proposed that in the peroxidase - luminol - perborate system at pH 7.3 , chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not , as generally assumed , from the reaction of luminol radicals with the molecular oxygen . - pubmed
Significantly higher frequencies of " disadvantage " alleles , usually combined with a higher LDL cholesterol , were found in hypercholesterolemic individuals when polymorphisms in apolipoprotein E , apolipoprotein B -- XbaI and LDL receptor -- PvuII were studied . - pubmed
Although the histogenesis of these intranuclear inclusions has not been clarified , they should not be mistaken for those in endometrial cells infected by herpesvirus because of intranuclear biotin and a false - positive histochemical reaction in the avidin - biotin - peroxidase complex method . - pubmed
Iba1 protein is therefore suggested to act as an adapter molecule , mediating calcium signals that may function in a monocytic lineage including microglia . - pubmed
Increasing 4 '- epidoxorubicin and fixed ifosfamide doses plus granulocyte - macrophage colony - stimulating factor in advanced soft tissue sarcomas : a pilot study . - pubmed
PURPOSE : To determine the maximum - tolerated dose ( MTD ) of 4 '- epidoxorubicin ( EPI ) in combination with full dose of ifosfamide ( IFO ) when granulocyte - macrophage colony - stimulating factor ( GM - CSF ) was used , to estimate its clinical efficacy , and to evaluate the mobilization of hematopoietic progenitors . - pubmed
The most relevant mobilization effect was obtained at the third EPI level , when both GM - CSF and IL-3 were used as in vitro - stimulating factors . - pubmed
It is commonly assumed that the role of the strongly activated heterosynaptic input during the induction of associative long - term potentiation ( LTP ) is to relieve the magnesium blockade of NMDA receptors located at the weakly stimulated synapses and thereby allow the weak input to undergo potentiation . - pubmed
The effect of releasing D - AP5 by flash photolysis of 100 microM caged D - AP5 ( N -[ 1 -( 2 - nitrophenyl ) ethoxycarbonyl ]- D - AP5 ) on pharmacologically isolated NMDA receptor - mediated field EPSPs was examined in area CA1 . - pubmed
Rapid application of D - AP5 , by flash photolysis of caged D - AP5 , coincident with the last shock of the strong tetanus , resulted in the blockade of NMDA receptor activation during the period of the weak tetanus . - pubmed
MMPs are a homologous family of zinc - dependent proteases . - pubmed
PVNx also lowered the depressor response to ganglionic blockade ( AL - PVNx=-28+/-5 mmHg , FR - PVNx=-29+/-4 mmHg ) and plasma norepinephrine levels ( AL - PVNx=372+/-74 pg / ml , FR - PVNx=248+/-31 pg / ml ) . - pubmed
Even though protection at the membrane level is conferred by the nitroxides during the early stages of lipid peroxidation , their antioxidative ability might be overwhelmed at a later stage by other mechanisms such as the increased autoxidation of hemoglobin in the presence of the nitroxides , thus giving a possible explanation for the early induction of hemolysis induced by the nitroxides . - pubmed
Finally , using in vitro binding studies , we showed that SREBP2 was able to displace ADD1 / SREBP1 binding from the sterol regulatory element ( SRE ) site . - pubmed
HAT activities acetylating either histone H3 ( SAGA , Ada , and NuA3 ) or H4 ( NuA4 ) stimulate HIV-1 transcription from preassembled nucleosomal templates in an acetyl CoA - dependent manner . - pubmed
His ... Asp catalytic dyad of ribonuclease A : conformational stability of the wild - type , D121N , D121A , and H119A enzymes . - pubmed
Residue His119 acts as an acid / base during the cleavage / hydrolysis reactions catalyzed by bovine pancreatic ribonuclease A ( RNase A ) . - pubmed
This His ... Asp dyad is conserved in all homologous pancreatic ribonucleases of known sequence . - pubmed
Accordingly , Asp121 was found to have pKa values of approximately 2.4 and 3.4 in the native and denatured states , respectively , of wild - type RNase A. - pubmed
We conclude that the His ... Asp catalytic dyad in pancreatic ribonucleases has two significant roles : ( 1 ) to position the proper tautomer of His119 for catalysis and ( 2 ) to enhance the conformational stability of the native enzyme . - pubmed
Asp121 of RNase A is a rare example of a residue that contributes equally to both . - pubmed
If catch - up growth can not be achieved by an alternate - day steroid regimen and discontinuation of glucocorticoids appears to be an intolerable risk for graft survival , rhGH therapy may be initiated . - pubmed
Co - expression of CD7 on CD34 + cells was induced to decrease significantly after short - term in vitro culture with the differentiation - inducing agent phorbol ester ( PMA ) and with a combination of cytokines ( stem - cell factor , interleukin-3 and granulocyte colony - stimulating factor ) . - pubmed
The loss of CD7 marker following incubation with PMA and cytokines suggests that this antigen is expressed transiently in early myeloid leukaemic CML haemopoiesis . - pubmed
Differential effects of cocaine and methamphetamine on neurotensin / neuromedin N and preprotachykinin messenger RNA expression in unique regions of the striatum . - pubmed
This study employed in situ hybridization to directly compare the effects of cocaine and methamphetamine on neurotensin / neuromedin N and preprotachykinin messenger RNAs in distinct striatal regions . - pubmed
Methamphetamine and cocaine produced significant increases in preprotachykinin messenger RNA in the striatum after 3h , but often in different subregions . - pubmed
However , methamphetamine produced significant increases in all regions of the caudal striatum , whereas cocaine - induced preprotachykinin messenger RNA expression was limited to dorsal regions of this striatal area . - pubmed
Methamphetamine also produced a significant increase in preprotachykinin messenger RNA in the caudal striatum after 30min , whereas cocaine had no significant effect on preprotachykinin messenger RNA at this early time - point . - pubmed
The pattern of changes in neurotensin / neuromedin N messenger RNA caused by methamphetamine and cocaine after 3h was even more distinct . - pubmed
Cocaine produced significant increases in neurotensin / neuromedin N messenger RNA in all regions of the rostral striatum , whereas methamphetamine had no effect in these areas . - pubmed
Furthermore , in more caudal sections , cocaine predominantly affected neurotensin / neuromedin N expression in dorsal aspects of the striatum , whereas methamphetamine significantly increased neurotensin / neuromedin N messenger RNA in all regions . - pubmed
The only significant effect was an increase in neurotensin / neuromedin N messenger RNA in the core region 3h after methamphetamine administration . - pubmed
These results indicate that methamphetamine and cocaine increase preprotachykinin and neurotensin / neuromedin N messenger RNAs in distinct regions of the striatum . - pubmed
Internalization of these variant receptors was dependent on the catalytic cysteine residue of Rsp5p and on ubiquitin - conjugating enzymes that bind Rsp5p . - pubmed
The crystal structure of the complex of the COOH - terminal fragment of the heavy chain with an analogue of its ganglioside receptor , GT1b , provides the first direct identification and characterization of the ganglioside - binding sites . - pubmed
The appearance of p32 was assessed by immunoblotting SDS - extracted and separated sperm proteins using an anti - phosphotyrosine antibody . - pubmed
An in - gel tyrosine kinase renaturation assay showed that TK-32 activity depends on calcium and bicarbonate in the incubation medium . - pubmed
Celecoxib treatment inhibited VEGF mRNA expression without any significant reduction in cyclooxygenase-2 mRNA . - pubmed
Thus , celecoxib reaches the retina after oral administration and reduces diabetes - induced retinal VEGF mRNA expression and vascular leakage by inhibiting the activity of cyclooxygenase-2 enzyme . - pubmed
SU5416 is reported to be a selective inhibitor of vascular endothelial growth factor , and it has metwith limited success in the clinic . - pubmed
Treatment of the ovarian carcinoma cells with SU5416 was also associated with a reduced expression of ERCC-1 protein and c - jun mRNA , as well as a decrease in c - Jun and JNK activities . - pubmed
The interconversion of 1 into 2 - 4 , as well as [ ( ( t ) Bu )( 2 ) Ga ( mu - OPh )]( 2 ) into 5 , and their reverse reactions , have been followed by ( 13 ) C CPMAS NMR spectroscopy , TG / DTA , SEM , EDX , and powder XRD . - pubmed
STUDY ON THE BIOGENESIS OF CATECHOLAMINES IN PHEOCHROMOCYTOMA TISSUE CULTURE . - pubmed
The recombinant alr0975 protein strongly catalyzed the first step of PC synthesis , in which glutathione ( GSH ) is converted to gamma - glutamylcysteine ( gamma - EC ) , although the protein only weakly catalyzed the second step of PC synthesis , namely the transfer of gamma - EC moiety to an acceptor GSH molecule to form PC ( 2 ) . - pubmed
Phosphotyrosyl peptides and analogues as substrates and inhibitors of purple acid phosphatases . - pubmed
Based on the observation that phosphotyrosine - containing peptides are good substrates for pig purple acid phosphatase , peptides containing a range of phosphotyrosine mimetics were synthesized . - pubmed
HR - TEM ( high - resolution transmission electron microscopy ) and selected - area EDS ( energy - dispersive spectroscopy ) show that the nanoparticles are homogenously spread in the ZnO tubes . - pubmed
Effect of crosslinking and drug loading on thermal decomposition of chitosan was studied by thermogravimetry ( TGA ) and these data indicated that pure chitosan is stable when compared to clozapine - loaded chitosan . - pubmed
OBJECTIVE : A missense mutation at codon 810 ( Ser --> ; Leu ) of the mineralocorticoid receptor was recently observed in a family with early manifestation of hypertension . - pubmed
Peak blood glucose ( BG ) and time to peak BG during PICU admission , duration of hyperglycemia ( percentage of PICU days with any BG of & gt;126 mg / dL ) , and intensity of hyperglycemia ( median BG during first 48 PICU hours ) were analyzed for association with PICU mortality using chi - square , Student 's t - test , and logistic regression . - pubmed
Impact of in ovo - administered lead and testosterone on developing female thymocytes . - pubmed
Using an avian in ovo model of lead - induced T - helper disruption , the ability of in ovo administered lead and testosterone to alter thymocyte maturation among female embryos was investigated . - pubmed
However , when testosterone treatment was followed by lead ( testosterone + lead ) , there was a significant increase in CD4+CD8 + double - positive cells compared with either control or lead treatment groups . - pubmed
Testosterone , either by itself or in combination with lead , significantly reduced the percentage of cells with the CD4+CD8- phenotype when compared to the lead alone group . - pubmed
We measured the gain of the aVOR elicited by rapid rotary head thrusts in each of the canal planes for each subject before and after treatment with intratympanic gentamicin by using magnetic search coils to record eye movements . - pubmed
Modification of GTPases with isoprenoid molecules derived from geranylgeranyl pyrophosphate or farnesyl pyrophosphate is an essential requisite for cellular signaling pathways . - pubmed
Both enzymes play crucial roles in cell survival , and inhibition of farnesyl pyrophosphate synthase by nitrogen - containing bisphosphonates is an established concept in the treatment of bone disorders such as osteoporosis or certain forms of cancer in bone . - pubmed
Our experiments demonstrate that a platinum cross - link locally overrides the rotational setting predefined in the nucleosome positioning sequence such that the lesion faces toward the histone core . - pubmed
The pifi mutant exhibited a lower capacity for nonphotochemical quenching , but similar CO ( 2 ) assimilation rates , photosystem II ( PSII ) quantum efficiencies ( PhiPSII ) , and reduction levels of the primary electron acceptor of PSII ( 1 - qL ) as compared with the wild type . - pubmed
Erythrocyte activity of transketolase , the concentrations of thiamine and related phosphorylated metabolites in plasma , erythrocytes and urine , and markers of metabolic control and vascular dysfunction were determined . - pubmed
Plasma thiamine concentration and urinary excretion of thiamine correlated negatively with soluble vascular adhesion molecule-1 ( r = -0.246 , p & lt ; 0.05 , and -0.311 , p & lt ; 0.01 , respectively ) . - pubmed
The conventional assessment of thiamine status was masked by increased thiamine transporter content of erythrocytes . - pubmed
Ornithine decarboxylase ( ODC ) , the rate - limiting enzyme in polyamine biosynthesis that decarboxylates ornithine to putrescine , has become a promising target for cancer research . - pubmed
RESULTS : PI3 K inhibitors wortmannin and LY294002 caused dose - dependent cellular and mitochondrial GSH depletion and downregulation of the modulatory subunit of GCL in cultured RPE cells . - pubmed
Both the basal and the induced Nrf2 activities were inhibited by wortmannin and LY294002 . - pubmed
LY294002 also inhibited sulforaphane - induced Nrf2 nuclear translocation . - pubmed
Samples of cortical tissue collected 24 h post METH treatment were pooled , processed and analyzed via a selective psychoproteomic platform . - pubmed
The beta - ketoacyl -( acyl carrier protein ) synthases ( beta - keto - ACP synthases ; KAS ) catalyse the addition of two - carbon units to the growing acyl chain during the elongation phase of fatty - acid synthesis . - pubmed
The dimeric state of TtKAS II is important to create each fatty - acid - binding pocket . - pubmed
Closer examination of KAS structures reveals that compared with other KAS structures in the apo form , the active site of TtKAS II is more accessible because of the ; open ' conformation of the Phe396 side chain . - pubmed
The cardinal features of AD include the formation of extracellular protein deposits in the brain that consist predominantly of aggregates of b amyloid protein(senile plaques ) , neurofibrilary tangles ( hyperfosforylated tau protein ) in the intracellular compartments , disturbances in calcium homeostasis , and degeneration / loss of synapses and neurons . - pubmed
Chronic activation of the microglia and astrocytes may cause damage of the brain - blood barrier and neuronal damage through the release of potentially cytotoxic molecules such as proinflammatory cytokines , reactive oxygen species , NO , and complement proteins . - pubmed
Gonadal steroid production is stimulated by gonadotropin binding to G protein - coupled receptors ( GPCRs ) . - pubmed
Although GPCR - mediated increases in intracellular cAMP are known regulators of steroidogenesis , the roles of other signaling pathways in mediating steroid production are not well characterized . - pubmed
This pathway is critical for LH - induced steroid production in ovarian follicles , probably through matrix metalloproteinase ( MMP)-mediated release of EGF receptor ( EGFR ) binding ectodomains . - pubmed
We demonstrated that , similar to the ovary , trans - activation of the EGF receptor was critical for gonadotropin - induced steroid production in Leydig cells . - pubmed
LH - induced increases in cAMP and cAMP - dependent protein kinase ( PKA ) activity mediated trans - activation of the EGF receptor and subsequent mitogen - activated protein kinase ( MAPK ) activation , ultimately leading to StAR phosphorylation and mitochondrial translocation . - pubmed
Steroidogenesis in Leydig cells was unaffected by MMP inhibitors , suggesting that cAMP and PKA trans - activated EGF receptors in an intracellular fashion . - pubmed
Interestingly , although cAMP was always needed for steroidogenesis , the EGFR / MAPK pathway was activated and necessary only for early ( 30 - 60 min ) , but not late ( 120 min or more ) , LH - induced steroidogenesis in vitro . - pubmed
Responses of limbic and extrapyramidal substance P systems to nicotine treatment . - pubmed
RESULTS : The nicotine treatment significantly but temporarily decreased substance P - like immunoreactivity ( SPLI ) content in the ventral tegmental area ( VTA ) and substantia nigra 12 - 18 h after drug exposure . - pubmed
The nicotine - mediated changes in SPLI were selectively blocked by pretreatment with mecamylamine as well as a dopamine D1 , D2 , or both receptor antagonists . - pubmed
CONCLUSIONS : These findings indicate that some limbic and basal ganglia SP systems are significantly affected by exposure to nicotine through processes mediated by nicotinic and dopaminergic receptors , suggesting a role for SP pathways in nicotine 's limbic and extrapyramidal effects . - pubmed
The responsive protein adsorption on four polyCBAAs was mapped out . - pubmed
MS medium supplemented with 1.5 mg L(-1 ) BA and 0.2 mg L(-1 ) NAA elicited the maximum number of shoots ( 4 multiple shoots ) from nodal explants . - pubmed
These adventitious shoots were best rooted on half strength MS medium supplemented with 1.0 mg L(-1 ) each of IBA and IAA . - pubmed
The pan - caspase inhibitor ( z - VAD - fmk ) completely blocked the pFPhe - mediated apoptotic changes except caspase-9 activation . - pubmed
Such findings confirm the importance of CTGF / CCN-2 in development and of the necessary and sufficient role of this molecule in formation of the skeleton , extracellular matrix and chondrogenesis . - pubmed
Acid polysaccharide - induced amorphous calcium carbonate ( ACC ) films : colloidal nanoparticle self - organization process . - pubmed
Amorphous calcium carbonate ( ACC ) plays important roles in biomineralization , and its synthesis in vitro has been of keen interest in the field of biomimetic materials . - pubmed
We extended previously developed quantitation of thoracic fat volume ( TFV ) from non - contrast coronary calcium ( CC ) CT scans to also quantify pericardial fat volume ( PFV ) and investigated the associations of PFV and TFV with CC and the Metabolic Syndrome ( METS ) . - pubmed
PFV and TFV were also associated with METS [ PFV : p<0.001 , OR 6.1 ; TFV p<0.001 , OR 5.7 ] , unlike CC [ OR=1.0 p = NS ] or RF . - pubmed
PFV correlated with low - HDL and high - glucose ; TFV correlated with low - HDL , low - adiponectin , and high glucose and triglyceride levels . - pubmed
Calcitonin - stimulated renal Ca2 + reabsorption occurs independently of TRPV5 . - pubmed
BACKGROUND : Calcitonin ( CT ) is known to affect renal Ca ( 2 + ) handling . - pubmed
However , it remains unclear how CT affects Ca ( 2 + ) transport in the distal convolutions . - pubmed
The aim of this study was to investigate the contribution of the renal epithelial Ca ( 2 + ) channel , transient receptor potential vanilloid 5 ( TRPV5 ) , to renal Ca ( 2 + ) handling in response to CT . - pubmed
In addition , urinary Na (+ ) and K (+ ) excretion also decreased after CT administration . - pubmed
Transient exposure of cardiac myocytes to hydrogen peroxide ( H ( 2 ) O ( 2 ) ) results in further production of superoxide by the mitochondria as a result of increased influx of calcium through the L - type Ca ( 2 + ) channel and increased calcium uptake by the mitochondria . - pubmed
Exposure of myocytes to 30 muM H ( 2 ) O ( 2 ) ( 5 min ) then 10 U / mL catalase ( 5 min ) increased dihydroethidium ( DHE ) signal by 58.7 + - 12.0 % ( n=4 ) compared to myocytes exposed to 0 muM H ( 2 ) O ( 2 ) for 5 min followed by 10 U / mL catalase ( n=9 ) . - pubmed
Complex I inhibitors DPI ( n=5 ) and rotenone ( n=7 ) attenuated the increase in DHE signal due to H ( 2 ) O ( 2 ) . - pubmed
However , antimycin A ( inhibitor of Q(i ) site of complex III ) had no effect . - pubmed
Direct activation of the L - type Ca ( 2 + ) channel by voltage - step mimicked the increase in DHE signal after transient exposure to H ( 2 ) O ( 2 ) ( 47.6 + - 17.8 % , n=6 ) while intracellular application of catalase attenuated the increase in DHE signal due to H ( 2 ) O ( 2 ) ( n=6 ) . - pubmed
In pairwise comparisons of complete genomic sequences and ORF C1 nucleotide and amino acid sequences , DuCVs of the same genotype were clearly distinguished from those of heterologous genotypes . - pubmed
Analysis of the amino acid sequences of the capsid protein exhibited the existence of 18 genotype - specific substitutions . - pubmed
Four of the novel HLA - C alleles encode amino acid changes at codons 41 , 42 , 55 or 200 which have not previously been reported to be polymorphic . - pubmed
Some of the new alleles encode novel codons and unique amino acid changes at polymorphic positions in the HLA - A ( codons 55 and 120 ) , HLA - C ( codons 151 , 153 and 176 ) , HLA - B ( codons 31 and 84 ) and HLA - DRB1 ( codon 47 ) loci . - pubmed
Astrocytes at seizure foci seem to not be a homogeneous population of cells , and in addition to typical glial fibrillary acidic protein , positive reactive astrocytes also include a population of neuron glia-2 - like cells The astrocytes in sclerotic hippocampi differ from those in nonsclerotic hippocampi in their membrane physiology , having elevated Na + channels and reduced inwardly rectifying potassium ion channels , and some having the capacity to generate action potentials . - pubmed
Furthermore , the CC genotype of the c.891C>T polymorphism in the GPX1 gene was associated with higher values of 8 - oxo - dG and GPX activity levels as compared to those for the CT - TT genotype . - pubmed
Liquorice and glycyrrhetinic acid increase DHEA and deoxycorticosterone levels in vivo and in vitro by inhibiting adrenal SULT2A1 activity . - pubmed
The mineralocorticoid effects of liquorice are mediated by the inhibitory effects of one of its active components glycyrrhetinic acid on 11beta - hydroxysteroid dehydrogenase type 2 . - pubmed
Here we have investigated the wider effects of oral liquorice on steroidogenesis focussing particularly on possible inhibitory effects of glycyrrhetinic acid on adrenal sulfotransferase activity . - pubmed
Cortisol and cortisone levels reflected expected inhibition of 11beta - hydroxysteroid dehydrogenase type 2 by glycyrrhetinic acid . - pubmed
Glycyrrhetinic acid inhibited cortisone and enhanced cortisol synthesis consistent with 11beta - hydroxysteroid dehydrogenase type 2 inhibition . - pubmed
Inhibition of deoxycorticosterone and dehydroepiandrosterone conjugation was apparent within 4 h of starting glycyrrhetinic acid treatment and was not associated with changes in the expression of SULT 2A1 mRNA . - pubmed
Herein , we consider PAL once more , this time testing ISM as a means to broaden the substrate scope of this lipase by studying bulky substrates of the type 2 - phenylalkanoic acid esters as substrates that are not accepted by the WT . - pubmed
Iron administered for treatment of anaemia resulted in a larger increase in haemoglobin than iron given for prevention , and the benefit was similar in hyper- or holoendemic and lower endemicity settings . - pubmed
Temperature dependent spectral properties of type - I and quasi type - II CdSe / CdS dot - in - rod nanocrystals . - pubmed
Megalencephalic leukoencephalopathy with subcortical cysts type 1 ( MLC1 ) due to a homozygous deep intronic splicing mutation ( c.895 - 226T > G ) abrogated in vitro using an antisense morpholino oligonucleotide . - pubmed
Its circulating concentration is maintained within a very tight physiologic range : 2.25 and 2.50 mmol / L. Under physiological conditions , the ionized calcium concentration is regulated by the parathyroid hormone ( PTH ) , and 1,25 ( OH )( 2 ) vitamin D through interactions on target organs such as kidney , bone and intestine . - pubmed
The main tools are specific standard biochemical tests able to assess calcium balance and renal function , and some specific biochemical tests , such as PTH , 25 ( OH ) vitamin D , and genetic sequencing , used to clarify the cause of hypercalcemia and , subsequently , to determine appropriate therapy . - pubmed
Once hypercalcemia is confirmed by ionized calcium measurement , the intact PTH assay plays a crucial role to differentiate PTH - mediated from non - PTH - mediated hypercalcemia . - pubmed
It is based on the oxidation of tetramethylbenzidine ( TMB ) , which is catalysed by haemoglobin and its derivatives . - pubmed
Bax and Bak are required for apogossypolone , a BH3 - mimetic , induced apoptosis in chronic lymphocytic leukemia cells . - pubmed
Non - canonical Smads phosphorylation induced by the glutamate release inhibitor , riluzole , through GSK3 activation in melanoma . - pubmed
In the present study , we demonstrate that riluzole inhibits AKT - mediated glycogen synthase kinase 3 ( GSK3 ) phosphorylation in melanoma cell lines . - pubmed
Because we have demonstrated that GSK3 is involved in the phosphorylation of two downstream effectors of transforming growth factor beta ( TGFbeta ) , Smad2 and Smad3 , at their linker domain , our aim was to determine whether riluzole could induce GSK3beta - mediated linker phosphorylation of Smad2 and Smad3 . - pubmed
We present evidence that riluzole increases Smad2 and Smad3 linker phosphorylation at the cluster of serines 245/250/255 and serine 204 respectively . - pubmed
Using GSK3 inhibitors and siRNA knock - down , we demonstrate that the mechanism of riluzole - induced Smad phosphorylation involved GSK3beta . - pubmed
The riluzole - induced Smad linker phosphorylation is mechanistically different from the Smad linker phosphorylation induced by TGFbeta . - pubmed
We further show that riluzole upregulates the expression of INHBB and PLAU , two genes associated with the TGFbeta signaling pathway . - pubmed
The non - canonical increase in Smad linker phosphorylation induced by riluzole could contribute to the modulation of the pro - oncogenic functions of Smads in late stage melanomas . - pubmed
Here , we report the pharmacological activity of a 39 amino acid CNP analog ( BMN 111 ) with an extended plasma half - life due to its resistance to neutral - endopeptidase ( NEP ) digestion . - pubmed
' One - pot ' synthesis of dihydrobenzo [ 4,5 ][ 1,3 ] oxazino [ 2,3 - a ] isoquinolines via a silver ( I )-catalyzed cascade approach . - pubmed
The strategy features an AgNO ( 3 ) catalyzed ' one - pot ' cascade process involving formation of two new C - N bonds and one new C - O bond . - pubmed
In addition , the contractile response induced by 1 muM phenylephrine increased from 6.8 + - 0.6 mN to 11.4 + - 0.4 mN ( P & lt ; 0.05 ) and 14.9 + - 1.4 mN ( P & lt ; 0.05 ) by insulin ( 1 IU / kg , intraperitoneal ) or CAPA treatment , respectively . - pubmed
In Group A , after producing fatigue , P di at 20 Hz stimulation increased significantly with a bolus injection ( 0.75 mg kg(-1 ) ) followed by continuous infusion of amrinone ( 2.5 , 5 and then 10mug kg(-1)min(-1 ) ) IV ( P<0.05).P di at 100 Hz stimulation increased significantly with an administration of amrinone ( 10mug kg(-1)min(-1 ) IV ( P<0.05 ) . - pubmed
Self - assembly structures of 1H - indazoles in the solution and solid phases : a vibrational ( IR , FIR , Raman , and VCD ) spectroscopy and computational study . - pubmed
Using broad - spectrum and specific MMP inhibitors , we establish that MMP-1 , but not MMP-2 and - 9 , plays a key role in 5 - azadC - enhanced cell invasion . - pubmed
We show that 5 - azadC induces MMP-1 expression through a transcriptional mechanism without affecting MMP-1 promoter methylation status . - pubmed
Finally , we demonstrate that 5 - azadC treatment increases the nuclear levels of Sp1 and Sp3 transcription factors , and modulates their recruitment to the MMP-1 promoter , resulting in chromatin remodeling associated to 5 - azadC - induced MMP-1 expression . - pubmed
MOD and MAD comparisons with previously reported spectra were 2.7 % and 9.7 % , respectively ( TASMIP ) , 0.1 % and 12.0 % , respectively [ R. Birch and M. Marshall , " Computation of bremsstrahlung x - ray spectra and comparison with spectra measured with a Ge(Li ) detector , " Phys . - pubmed
A close correlation between copeptin concentrations and serum osmolality existed in 68 healthy controls , with a mean osmotic threshold+-SD of 282 + -4 mOsM / kg H2O. - pubmed
Prognostic and predictive value of metabolic tumor volume on ( 18 ) F - FDG PET / CT in advanced biliary tract cancer treated with gemcitabine / oxaliplatin with or without erlotinib . - pubmed
This study aimed to evaluate the prognostic significance and predictive performance of volume - based parameter of ( 18 ) F - fluorodeoxyglucose ( ( 18 ) F - FDG ) positron emission tomography / computed tomography ( PET / CT ) in biliary tract cancer ( BTC ) . - pubmed
The bioaccessibility of salicylic acid ( SA ) can be effectively modified by incorporating the pharmacological compound directly into polymers such as poly ( anhydride - esters ) . - pubmed
After simulated digestion conditions , the bioaccessibility of SA was observed to be statistically different ( p & lt ; 0.0001 ) in each sample : 55.5 + - 2.0 % for free SA , 31.2 + - 2.4 % the SA - diglycolic acid polymer precursor ( SADG ) , and 21.2 + - 3.1 % for SADG - P ( polymer ) . - pubmed
In the quantitative study , cancerous tissue with Gleason score at least 3 + 3 was distinguished from normal tissue in the peripheral zone ( PZ ) with an average AUC of 0.75 ( Gd ) , 0.75 ( Gl ) , 0.70 ( Gamma - Gd ) , 0.68 ( apparent diffusion coefficient , ADC ) , and 0.82 ( Gd + Gl + ADC ) . - pubmed
The differentiation between PZ and central gland was modest for Gd ( p & lt ; 0.07 ) , Gl ( p & lt ; 0.06 ) but not significant for Gamma ( p & lt ; 0.2 ) . - pubmed
The mRNA expression level of URAT1 was positively correlated with the concentration of uric acid but , negatively correlated with the expression level of miR-34a . - pubmed
INTRODUCTION : Melatonin , secreted by the pineal gland during the night phase , is a regulator of the biological clock and sleep tendency . - pubmed
The potentially beneficial effects of PRM persisted during the 2 weeks of discontinuation period , consistent with clock stabilizing effects . - pubmed
Selected Ion Flow Tube Mass Spectrometry ( SIFT - MS ) was used to quantify methanol and other volatile compounds in the headspace of one bacterial and 12 fungal lignin - degrading microbial cultures . - pubmed
Analysis was done using SIFT - MS with H3O (+ ) and NO (+ ) precursors . - pubmed
SIFT - MS can be used to quantify methanol and other volatile compounds in the headspace of microbial cultures and has the potential to be a rapid , sensitive , non - invasive tool useful in elucidating the mechanisms of lignin degradative pathways . - pubmed
We evaluated AMG 416 , a long - acting peptide agonist of the calcium - sensing receptor , to assess its safety , tolerability , and efficacy and to determine a safe and effective starting dose for subsequent phase 2 studies . - pubmed
The study was not designed to titrate AMG 416 dosing to achieve a specific PTH treatment goal . - pubmed
RESULTS : Analysis of the primary endpoint showed that treatment with AMG 416 at 10 mg ( Cohort 2 ) and 5 mg ( Cohort 3 ) for up to 4 weeks resulted in mean 49.4 % and 33.0 % reductions from baseline in PTH during the efficacy assessment phase , respectively ( p & lt ; 0.05 for both cohorts compared to placebo group within the cohort ) . - pubmed
Meanwhile , PSF intervened in the PID - associated alterations in TCA cycle , sugar metabolism , amino acid metabolism , and other uncertain metabolic pathways . - pubmed
The photoluminescence ( PL ) intensity of TGA - capped QDs is higher than that of MPA - capped QDs at same QD concentration because of the surface passivation of TGA . - pubmed
To systemically investigate the photodegradation , CdTe QDs with various PL peak wavelengths were dispersed in phosphate buffered saline ( PBS ) and Tris - borate - ethylenediaminetetraacetic acid ( TBE ) buffer solutions . - pubmed
As for TGA - capped CdTe QDs , the results of PL peak wavelengths in TBE buffer solutions indicated that S ( 2 - ) released by TGA attached to Cd ( 2 + ) and formed CdS - like clusters layer on the surface of aqueous CdTe QDs . - pubmed
Blood glucose was measured every two weeks , and insulin and glucose sensitivity tests were assessed at the end of the follow - up . - pubmed
All strains contained Q-10 as the predominant ubiquinone and C18 : 1omega7c as the major fatty acid . - pubmed
Adenosine deaminases acting on RNA ( ADARs ) are editing enzymes that convert adenosine to inosine in duplex RNA , a modification reaction with wide - ranging consequences in RNA function . - pubmed
A diketopyrrolopyrrole - based co - polymer ( PDPP - TAT ) was used as the semiconductor and poly ( methyl methacrylate ) was used as the gate insulator . - pubmed
FDG - PET and PET / CT in Colorectal Cancer . - pubmed
This article reviews the current use of FDG PET / CT for assessment of colorectal cancer . - pubmed
The current place of FDG PET / CT is discussed in light of recently published studies . - pubmed
Gating Modulation of the Tumor - Related Kv10.1 Channel by Mibefradil . - pubmed
Given that mibefradil ( Mb ) is also known to inhibit K + channels , we decided to study the interaction between this organic compound and the tumor - related Kv10.1 channel . - pubmed
Genetic polymorphisms of N - acetyltransferase type 2 ( NAT2 ) cause a trimodal distribution of INH pharmacokinetics in slow , intermediate , and fast acetylators . - pubmed
To mechanistically assess the trade - off between toxicity and efficacy , we developed a physiologically based pharmacokinetic ( PBPK ) model describing the NAT2 - dependent pharmacokinetics of INH and its metabolites . - pubmed
Subsequently , we evaluated various INH dosing regimens in NAT2 - specific immunocompetent and immune - deficient virtual populations . - pubmed
When FFM , Vitamin D intake , cBPAQ , pBPAQ , and tBPAQ were included in a stepwise multiple linear regression analysis , FFM and pBPAQ were predictors of total hip , accounting for 16 % ( p = 0.024 ) , while FFM and tBPAQ predicted 14 % of the variance in FN ( p = 0.015 ) . - pubmed
There was a positive correlation between Vitamin D intake and L2 - L4 ( p = 0.025 ) , but other dietary intakes variables were not significant ( p & gt ; 0.05 ) . - pubmed
Wide Distribution of Genes for Tetrahydromethanopterin / Methanofuran - Linked C1 Transfer Reactions Argues for Their Presence in the Common Ancestor of Bacteria and Archaea . - pubmed
Since d6 - acetone can be used as a starting material , the route developed allows for the facile incorporation of isotopic labels which are required for detecting and quantifying trace amounts via GC / MS analysis . - pubmed
Flavodiiron proteins ( FDPs ) are present in organisms from all domains of life and have been described so far to be involved in the detoxification of oxygen or nitric oxide ( NO ) , acting as O2 and / or NO reductases . - pubmed
Biochemical and in vivo studies revealed that FlRd is involved in NO detoxification as part of the bacterial defense mechanisms against reactive nitrogen species . - pubmed
coli FlRd has a clear preference for NO as a substrate in vitro , exhibiting a very low reactivity toward O2 . - pubmed
The overall tetrameric structure revealed a highly conserved flavodiiron core domain , with a metallo - beta - lactamase - like domain containing a diiron center , and a flavodoxin domain with a flavin mononucleotide cofactor . - pubmed
The dominant fatty acids of strain HQYD1 T were identified as C16 : 0 , C18 : 0 and iso - C15 : 0 . - pubmed
TG and GSH concentrations , together with gamma - GCS and gamma - GT activities , were significantly lower , while GSSG content and the GRR higher , in the AR than in the YACR aortas ; GSSG - Red activity did not differ significantly between the two groups . - pubmed
Heightened levels of lipid and protein oxidation and H2O2 occurred in the AR aortas , indicating age - dependent vascular oxidative stress . - pubmed
Association of A1538 G and C2437 T single nucleotide polymorphisms in heat shock protein-70 genes with diabetic nephropathy among South Indian population . - pubmed
We report a case wherein TTP was successfully treated with repeated plasma exchange ( PE ) and MPO - ANCA - associated vasculitis with corticosteroids . - pubmed
However , some non - sensitizing irritants , such as sodium dodecyl sulfate ( SDS ) or methyl salicylate ( MS ) , have been reported as false - positives by this endpoint . - pubmed
In search of a potential endpoint to enhance the specificity of existing endpoints , we evaluated 3 contact sensitizers ; ( hexyl cinnamic aldehyde [ HCA ] , oxazolone [ OXA ] , and 2,4 - dinitrochlorobenzene [ DNCB ] ) , 1 respiratory sensitizer ( toluene 2,4 - diisocyanate [ TDI ] ) , and 2 non - sensitizing irritants ( MS and SDS ) by several endpoints in LLNA . - pubmed
Cells transfected with TIRAP , IRAK1 , IRAK4 or TAK1 all showed a decrease in fsl-1 - induced expression of cytokines ( IL-1alpha , IL-1beta , IL-6 ) , chemokines ( GRO - alpha , IL-8 , MCP-1 ) , adhesion molecule ICAM-1 , cyclooxygenase ( COX )- 2 mRNA and release of PGF2alpha and MMP-9 expression . - pubmed
The proliferation , and migration and invasion capacities of prostate cells were determined by EdU and Transwell assays following transfection with BLM - targeting short hairpin RNA ( shRNA ) . - pubmed
, gefitinib in the maintenance setting irrespective of EGFR status . - pubmed
OBJECTIVE : The aim was to evaluate the effect of short glass - fiber / filler particles proportion on fracture toughness ( FT ) and flexural strength ( FS ) of an experimental flowable fiber - reinforced composite ( Exp - SFRC ) with two methacrylate resin formulations . - pubmed
While sugar sweetened beverage ( SSB ) consumption has declined in the last 15 years , consumption of SSBs is still high among children and adolescents . - pubmed
Herein , we demonstrate the 1 T ' transition metal telluride atomic layers ( WTe2 and MoTe2 ) as ultrasensitive platforms for CM - based SERS . - pubmed
To circumvent these obstacles , we anchored CsPbBr3 QDs ( CPB ) on NHx -rich porous g - C3 N4 nanosheets ( PCN ) to construct the composite photocatalysts via N - Br chemical bonding . - pubmed
The obtained results demonstrated that fipronil may undergo a clearance by the liver and it is exclusively metabolized by the CYP3A4 isoform . - pubmed
To obtain new insight into parasite sphingolipid catabolism , the substrate specificity of TbnSMase was investigated using electrospray ionization tandem mass spectrometry ( ESI - MS / MS ) . - pubmed
Recombinant TbnSMase was shown to degrade sphingomyelin , inositol - phosphoceramide and ethanolamine - phosphoceramide sphingolipid substrates , consistent with the sphingolipid complement of the parasites . - pubmed
TbnSMase also catabolized ceramide-1 - phosphate , but was inactive towards sphingosine-1 - phosphate . - pubmed
The broad - range specificity of this enzyme towards sphingolipid species is a unique feature of TbnSMase . - pubmed
Additionally , ESI - MS / MS analysis revealed previously uncharacterized activity towards lyso - phosphatidylcholine despite the enzyme 's inability to degrade phosphatidylcholine . - pubmed
AIM : The secretolytic drug ambroxol may be useful for the treatment of neuropathic pain due to its multiple modes of action . - pubmed
Calcineurin inhibitors , cyclosporine and tacrolimus , and everolimus are used to prevent reactions following organ transplantation . - pubmed
Quantitative real - time polymerase chain reaction results confirmed the alterations in the expression levels of genes encoding components of pathways involved in phenylpropanoids and lipids . - pubmed
Moreover , PFB@PLLA loads high amount of oxygen and US - triggering PFB@PLLA reoxygenation effectively inhibits the expression of hypoxia - related proteins ( HIF-1alpha and CAIX ) , reduces lactate secretion and glycolysis , which modulates hypoxic microenvironment and inhibits cancer cell migration and invasion in vitro . - pubmed
Sodium dichloroacetate ( DCA ) is a mitochondrial pyruvate dehydrogenase kinase inhibitor , and has been shown to display vasoprotective effects in chronic ischemic stroke . - pubmed
N 4 - Hydroxycytidine ( NHC ) is an antiviral ribonucleoside analog that acts as a competitive alternative substrate for virally encoded RNA - dependent RNA polymerases . - pubmed
The mitochondrial DNA - dependent RNA polymerase ( POLRMT ) has been shown to incorporate some nucleotide analogs into mitochondrial RNAs , resulting in substantial mitochondrial toxicity . - pubmed
We demonstrate that the 5 '- triphosphate of NHC can be used by POLRMT for incorporation into nascent RNA chain but does not cause immediate chain termination . - pubmed
HbII and HbIII contain B10 tyrosine ( Tyr ) and E7 glutamine ( Gln ) in the heme pocket generating an efficient hydrogen bonding network with the ( HbII - HbIII)-O2 species , leading to very low ligand dissociation rates . - pubmed
The results suggest that acidic pH generates conformational changes associated with B10 and E7 heme pocket amino acids , weakening the ( HbII - HbIII)-O2 hydrogen bond network . - pubmed
The sodium / iodide symporter ( NIS ) has positive expression in thyroid carcinomas with good prognoses and plays a critical role in radioiodine therapy response . - pubmed
Peroxisome proliferator - activated receptor gamma ( PPARgamma ) agonists and retinoids are two types of redifferentiating agents . - pubmed
Moreover , rosiglitazone treatment reduced cell growth and increased NIS protein expression in thyroid cancer cells under normoxic or hypoxic conditions . - pubmed
In addition , bexarotene potentiated the effects of rosiglitazone on cell growth and NIS protein expression . - pubmed
Here , we present a step wise protocol to ( a ) measure Ca2 + interaction with the Ca2 + -sensor guanylate cyclase - activating protein 1 , ( b ) to design an ITC experiment and prepare samples , ( c ) to remove Ca2 + nearly completely from Ca2 + binding proteins without using a chelating agent like EGTA . - pubmed
Haemoglobin DIbadan ( beta 87 threonine leads to lysine ) was originally found in Ibadan , an area with a high incidence of sickle cell haemoglobin ( HbS ) . - pubmed
However , the EDTA test showed absent or low responses in 10 of 11 cases studied with GP 62 . - pubmed
In chronic renal failure normal responses can be observed despite an abnormal basal PTH level , since it is falsely elevated by the accumulation of COOH - terminal fragments . - pubmed
Additional studies exhibited post - transcriptional regulation by phorbol ester . - pubmed
DMSO was also shown to regulate c - myb at the post - transcriptional level . - pubmed
Interestingly , the post - transcriptional regulation of c - myb by DMSO required continuous transcription . - pubmed
To determine whether aromatase activity of cultured granulosa cells obtained from relatively low estradiol ( E2 ) responders ( serum E2 & lt ; 1000 pg / ml ) to hMG therapy differed from that of good responders ( E2 & gt ; or = 1000 pg / ml ) , we prospectively compared serum E2 on the day of human chorionic gonadotropin administration to in vitro aromatase activity following a 72 - h culture . - pubmed
Benzodiazepine receptor ligands and hepatic encephalopathy : further unfolding of the GABA story . - pubmed
Addition of 0.5 mumol / l MCLA and a stimulatory agent , such as N - formyl - methionyl - leucyl - phenylalanine ( fMLP ) and phorbol myristate acetate ( PMA ) , to a suspension of the cells treated with gamma interferon ( IFN gamma ) caused a marked luminescence which was inhibited by 0.5 mumol / l superoxide dismutase ( SOD ) . - pubmed
On the other hand , the cells which were not treated with IFN gamma caused no significant luminescence by the stimulation with either fMLP or PMA . - pubmed
Surprisingly , rCGCUUGCG forms a very stable duplex at pH 7 in 1 M NaCl with a TM of 44.8 degrees C at 1 x 10(-4 ) M and a delta G degrees 37 of -7.2 kcal / mol . - pubmed
At neutral pH , rCGCCCGCG is one of the least stable duplexes with a TM of 33.2 degrees C and delta G degrees 37 of -5.1 kcal / mol . - pubmed
rCGCCAGCG and rGGCACGCC have a small pH dependence , with TM increases of 5 and 3 degrees C , respectively , upon lowering the pH from 7 to 5.5 . - pubmed
rCGCCUGCG and rCGCUCGCG also show little pH dependence , with TM increases of 0.8 and 1.4 degrees C , respectively , upon lowering the pH to 5.5. - pubmed
Antibodies raised against purified P-450IIIA inhibited the microsomal formation of quantitatively minor cannabinoid metabolites but had no effect on the major metabolites . - pubmed
Unlike anti - P-450IIIA antibody , antibody raised against purified P-450THC markedly inhibited the microsomal formation of all major cannabinoid metabolites . - pubmed
The significance of its beta 1 - adrenoceptor selectivity was examined at various cardiovascular activation levels established by either incremental isoprenaline infusion or graded treadmill exercise . - pubmed
Characteristically of a beta 1 - selective agent , esmolol had only a slight effect on the isoprenaline - induced reduction in diastolic blood pressure . - pubmed
beta - Adrenoceptor blockade was virtually reversed within 20 min of discontinuation of esmolol infusion . - pubmed
The ranking order was RRR - alpha - T greater than RRR - beta - T greater than RRR - gamma - T greater than or equal to R - alpha - TT greater than RRE - delta - T. Accordingly , the biopotency of a palm - oil residue was assessed and expressed as alpha - tocopherol equivalents ( alpha - TEs ) . - pubmed
The release of pyruvate kinase , a variable in the nutrition - linked necrotizing myopathy , into the plasma was dose - dependently inhibited by the RRR - alpha - T standard and the corresponding alpha - TE from this residue . - pubmed
Prostacyclin synthesis from aorta segments induced by thrombin or ionomycin was higher than the spontaneous release . - pubmed
However , there was no difference between the depleted group and groups treated with RRR - alpha - T or alpha - TEs from the palm - oil residue . - pubmed
Upon supplementation with RRR - alpha - T or alpha - TEs from the palm - oil residue , reduced IgG concentrations were observed , similar to those of animals on a commercial diet containing adequate amounts of vitamin E. - pubmed
Before entry they circumvent the lytic activity of the complement cascade , during cell entry they avoid being killed by toxic oxygen metabolites and after entry they escape nonoxidative killing mechanisms such as degradation by lysosomal hydrolases . - pubmed
PAG - DRP with properties of spatial and temporal summation propagates decrementally along the dorsal root and is inhibited by picrotoxin . - pubmed
In addition , the possibility that colonic glucuronyltransferase might be involved in the biotransformation of MAM was also investigated . - pubmed
MAM inhibition of colonic 4 - methylumbelliferone glucuronyltransferase was non - competitive , suggesting indirectly that colonic glucuronyltransferase is not involved in conjugation of MAM . - pubmed
Temporal relationships among fetal urine flow , ANF , and AVP responses to hypertonic infusions . - pubmed
The fetal urine flow response to acute increases in osmolality may be mediated by changes in the plasma concentrations of atrial natriuretic factor ( ANF ) , arginine vasopressin ( AVP ) , and/or angiotensin II ( ANG II ) . - pubmed
Thus the initial increase in fetal urine flow in response to acute hypertonic infusions is temporally related to a rise in fetal plasma ANF , whereas the subsequent fall in urine flow is temporally related to a fall in plasma ANF and a simultaneous rise in AVP concentration . - pubmed
This suggests that ANF may contribute to the acute urine flow increase after hypertonic infusion , whereas AVP appears to be more important for the long - term regulation of fetal urine flow . - pubmed
coli dnaK gene product , the hsp70 homolog , can reactivate heat - inactivated RNA polymerase in an ATP hydrolysis - dependent manner . - pubmed
coli hsp70 homolog , the dnaK gene product , protects the host RNA polymerase enzyme from heat inactivation in an ATP - independent reaction . - pubmed
In addition , we show that heat - inactivated and aggregated RNA polymerase is both disaggregated and reactivated following simultaneous incubation with DnaK protein and hydrolyzable ATP . - pubmed
This study was aimed at evaluating the effect of diltiazem , a calcium channel blocking agent , on myotonia induced by 2,4 - dichlorophenoxyacetic acid ( 2,4 - D ) . - pubmed
Influence of D - glucose upon the respiratory and secretory response of insulin - producing tumor cells to 2 - aminobicyclo [ 2,2,1 ] heptane-2 - carboxylic acid . - pubmed
The metabolic and secretory effects of 2 - aminobicyclo [ 2,2,1 ] heptane-2 - carboxylic acid are opposed to those of D - glucose , which increases respiration , ATP content , and insulin release , while lowering NH4 + production and 14CO2 output from the prelabeled cells . - pubmed
These findings suggest that , in tumor as in normal islet cells , the regulation of insulin release by exogenous nutrients depends on the availability of endogenous ATP . - pubmed
Atrial concentration of i alpha - rANP was significantly lower in the DOCA - salt group than in the other groups at 1 week , and was reduced in the DOCA and DOCA - salt groups as compared to the control group at 8 weeks . - pubmed
On the other hand , plasma concentration of i alpha - rANP was significantly higher in the DOCA and the DOCA - salt groups than in the control group at 1 week ; the DOCA and DOCA - salt group values were also higher than the control and salt group values at 8 weeks . - pubmed
In the present study , fragments of proliferative and secretory phase endometrium were cultured in media in the presence and absence of progesterone ( P ) and 17 beta - estradiol ( E2 ) for 96 h. The PP12 concentrations in the media and tissues were measured by RIA , and de novo synthesis was investigated by measuring the incorporation of [ 35S ] methionine into PP12 . - pubmed
All secretory endometria released PP12 into the medium in the presence and absence of added P and E2 . - pubmed
The enthalpy of dilution of alpha - chymotrypsin and of proflavin , and the enthalpy of binding of indole , N - acetyl - D - tryptophan , and proflavin to alpha - chymotrypsin . - pubmed
The findings suggest that the prejunctional inhibitory effect of methysergide may be mediated via stimulation of a 5 - HT receptor which , unlike the D - receptor , is not blocked by cyproheptadine . - pubmed
In dispersed mucosal cells from guinea - pig stomach , somatostatin inhibited in a noncompetitive fashion ( Ki , 2 x 10(-8 ) M ) the increase in cellular cyclic AMP caused by histamine but not by prostaglandin E1 or phosphodiesterase inhibitors . - pubmed
Neoplastic brain levels of the Photofrin II isotope were higher than in the HpD studies , and highly fluorescent . - pubmed
Epidermal growth factor stimulates tyrosine phosphorylation of the myosin regulatory light chain from smooth muscle . - pubmed
Two moles of phosphate are incorporated into tyrosine per mol of LC20 . - pubmed
Tyrosine 142 is located within a sequence similar to that of autophosphorylated tyrosine kinases in that five of the seven amino acids on the NH2 - terminal side are acidic . - pubmed
The possibility that a lysine - rich protein was involved in the acute stimulation of steroidogenesis by ACTH was investigated using [ 3H ] labelled lysine and isolated adrenal cells . - pubmed
However cells incubated in a lysine - free medium showed the same response to ACTH as cells incubated in a lysine - containing medium . - pubmed
It was also demonstrated that ACTH had no effect on the incorporation of tritiated lysine into the protein or small peptide fractions . - pubmed
These observations suggest that a rapidly synthesised , lysine - rich protein is not involved in the acute response to ACTH . - pubmed
The influence of oxygen donor ligation on the spectroscopic properties of ferric cytochrome P-450 : ester , ether and ketone co - ordination to the haem iron . - pubmed
Homogeneous low - spin complexes of cytochrome P-450 - CAM with esters , ethers and ketones have been prepared and characterized by u.v.-visible absorption , circular dichroism ( CD ) , magnetic circular dichroism ( MCD ) and electron paramagnetic resonance ( EPR ) spectroscopy . - pubmed
Anomalous spectral and substrate binding properties have been reported in the study of cytochrome P-450 under conditions employing solvents and non - phosphate buffers containing oxygen functionalities , and have been attributed to ' solvent effects ' . - pubmed
The present work , in combination with our previous report of alcohol , amide and carboxylate oxygen donor complexes of cytochrome P-450 , is evidence that a wide variety of oxygen - donor species are capable of direct ligation to the haem iron of cytochrome P-450 . - pubmed
This leads us to suggest oxygen - donor ligation to cytochrome P-450 as the origin of spectral and substrate binding anomalies previously attributed to solvent effects . - pubmed
The T lymphocyte - oriented mitogens concanavalin A and phytohemagglutinin and the B lymphocyte - oriented mitogen lipopolysaccharide were used to study variations in [ 3H ] thymidine incorporation in lymphocytes cultured in media deficient in one mineral element . - pubmed
To assess effects of industrial and environmental pollution on analytical characteristics of humic substances , we isolated humic acids ( HA 's ) and fulvic acids ( FA 's ) from unpolluted and polluted soils and sediments . - pubmed
Following purification , the HA 's and FA 's were characterized by elemental ( C , H , O , N , S ) and functional group ( CO2H , phenolic OH , total acidity ) analyses , infrared ( IR ) spectrophotometry , differential thermal analysis ( DTA ) and by metal ( Fe , Al , Cu , Mn , Pb , Ni , Co , Zn , Cr , Cd , Hg , Ca and Mg ) analyses . - pubmed
The effect of L - tryptophan on motor activity and its prevention by an extracerebral decarboxylase inhibitor and by 5 - HT receptor blockers . - pubmed
A further indication that the behavioural effect of tryptophan was not due to increased brain 5 - HT was its prevention by R04 - 4602 at a dose sufficient to block peripheral but not central L - aromatic amino acid decarboxylase . - pubmed
The adsorbed von Willebrand factor can be eluted from fibrillar collagen with 1 M NaCl . - pubmed
Heparin also inhibited platelet aggregation , release of ( 14C ) 5 - HT and production of malondialdehyde in response to collagen and thrombin . - pubmed
The results indicate that in addition to the effects of heparin on platelet function mediated by anti - thrombin activity and the previously described augmentation of responses to ADP , heparin has weak inhibitory activity against platelet - collagen interactions . - pubmed
NOS activity detected by [ 3H ] arginine to [ 3H ] citrulline conversion rose 109 % . - pubmed
The increase in NOS - III expression does not explain the declines in pulmonary endothelial NO production previously observed following prolonged hypoxia in this model . - pubmed
Alternatively , the fall in NO production may be related to diminished NOS co - factor availability . - pubmed
Relaxation to A-23187 , but not ADP , was abolished by removal of the endothelium or exposure to 100 microM NO2 - L - arginine.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
Moreover , a pronounced increase in ALA - B concentrations occurred when the inhibition rate of erythrocyte ALA dehydratase exceeded 85 % . - pubmed
A long biotin ester provided more effective binding of the B - RBC to immobilized avidin and greater lysis by complement , than a short biotin ester . - pubmed
The mechanisms involved in such effects were evaluated by measuring pituitary GH mRNA levels and hypothalamic levels of GH - releasing hormone ( GHRH ) and somatostatin ( SRIF ) mRNAs . - pubmed
in pups of either sex , from birth until 10 days of age , caused a significant increase in baseline plasma GH and potentiated the GH response to the [ Met5 ]- enkephalin analog FK 33 - 824 ( 1 mg / kg , s.c . ) - pubmed
, 3 h before ) did not change significantly basal GH levels but potentiated FK 33 - 824 ( 0.3 mg / kg , intracarotid)-induced stimulation of GH secretion . - pubmed
, for 3 days ) failed to modify basal and FK 33 - 824 - induced GH secretion , caused a significant reduction in hypothalamic GHRH mRNA levels and left SRIF and GH mRNAs unchanged . - pubmed
[ Effectiveness and tolerability of oral liquid ferrous gluconate in iron - deficiency anemia in pregnancy and in the immediate post - partum period : comparison with other liquid or solid formulations containing bivalent or trivalent iron ] . - pubmed
It is also now clear that women who take iron supplements during pregnancy do not suffer the same post - natal reduction in hemoglobin and ferritin as those who do n't make it . - pubmed
RESULTS : Analysis of the therapeutic efficacy parameters ( red blood cells , hemoglobin , hematocrit and serum iron ) showed significant improvements but no statistically significant differences between the groups . - pubmed
This result supports the hypothesis that DME , together with pyruvate dehydrogenase , forms a pathway in which malate is converted to acetyl - CoA. - pubmed
Interaction of bromocresol green with different serum albumins studied by fluorescence quenching . - pubmed
The binding of bromocresol green to bovine serum albumin at micromolar concentrations leads to quenching of protein fluorescence . - pubmed
This property has been used here to study interaction of bromocresol green with bovine serum albumin as a function of pH and ionic strength . - pubmed
The affinity of bromocresol green for bovine serum albumin remains virtually unchanged between pH 4.0 and 8.0 but decreases by about 7 fold with increase in ionic strength from 0.01 to 1.0 . - pubmed
Although all the albumins studied bind bromocresol green , they show considerable differences in their affinities towards the dye . - pubmed
However , there was no correlation between the non - GP GBM IgM antibody levels and the decrease in GFR as measured by Cr EDTA clearance . - pubmed
Gonadal steroids are known to alter GH secretion as well as tissue metabolism . - pubmed
The present study was designed to examine the effects of short term ( 2- to 3 - week ) alterations in gonadal steroids on basal pulsatile ( nonstimulated ) and exercise- and GH - releasing hormone - stimulated GH secretion , urinary nitrogen excretion , and basal and exercise - stimulated oxygen consumption . - pubmed
Raising testosterone to supraphysiological levels increased pulsatile GH secretion by 62 % over that with leuprolide and by 22 % over that with saline ( P & lt ; 0.05 ) . - pubmed
High dose testosterone treatment also increased serum insulin - like growth factor I concentrations by 21 % and 34 % over those during the eugonadal and hypogonadal states , respectively ( P & lt ; 0.01 ) . - pubmed
Testosterone did not affect either exercise- or GRF - stimulated GH secretion . - pubmed
In protocol 2 , stanozolol did not affect any parameter of GH secretion . - pubmed
To examine the interaction between GH secretion and testosterone on urinary nitrogen excretion and basal metabolic rate , a one - way analysis of covariance was undertaken . - pubmed
Statistical examination of GH production as the covariate and testosterone ( by tertile ) as the interactive factor demonstrated significant relationships between serum testosterone levels and either urinary nitrogen ( P & lt ; 0.02 ) or basal metabolic rate ( P & lt ; 0.01 ) , but not GH secretion ( P = NS ) . - pubmed
In summary , these results demonstrate that short term modulation of the androgen milieu affects metabolic outcome without necessitating changes in GH secretion . - pubmed
We also measured the glucose and insulin response to an oral glucose tolerance test and the insulin sensitivity ( [ SI ] by modified minimal model analysis ) . - pubmed
Measures of overall obesity ( BMI ) , total fat [ TF ] , and/or SCF measured at the abdomen by MRI ) , glucose / insulin metabolism and SI , and central fat patterning ( WHR or IAF measured by MRI ) were correlated with mean arterial pressure ( MAP ) , triglyceride ( TG ) , and high - density lipoprotein cholesterol ( HDL - C ) levels in univariate analysis and after controlling for age and gender . - pubmed
No index of overall obesity , fat patterning , glucose / insulin metabolism , and/or SI , was independently related to the plasma concentration of HDL - C after controlling for any one of the other two . - pubmed
Direct measurement of glucose / insulin metabolism and SI , as well as fat patterning , provides information on their relative associations with CVD risk factors . - pubmed
The measures of glucose / insulin metabolism and SI were more consistently related to dyslipidemia and hypertension than were the overall obesity and fat patterning in this healthy population . - pubmed
These conversions were purely biocatalytic , since in acidified water ( pH & lt ; 3.5 ) almost 50 % linalool was recovered unchanged , the rest was lost by evaporation . - pubmed
It was therefore hypothesized that expression of MgtA and MgtCB is increased upon invasion of eukaryotic cells because of a lack of intravacuolar Mg2 + . - pubmed
typhimurium was grown at pH 5.2 , the capacity of the constitutive CorA transporter in mediating Mg2 + was greater than that at pH 7.4 . - pubmed
typhimurium could obtain sufficient Mg2 + via the CorA system . - pubmed
Compared to induction at pH 7.4 , exposure to pH 5.2 almost completely abolished induction of mgtA at low Mg2 + concentrations but diminished induction of mgtCB only twofold . - pubmed
Adaptation of cells to acid pH by overnight growth resulted in normal levels of induction of mgtA and mgtCB at low Mg2 + concentrations . - pubmed
Conversely , repression of mgtA and mgtCB expression by increased extracellular Mg2 + was relatively insensitive to acid . - pubmed
After adjustment for age , sex , thallium perfusion defects , and other confounders , failure to reach 85 % of the age - predicted maximum heart rate was associated with increased risk of death ( adjusted relative risk [ RR ] , 1.84 ; 95 % confidence interval [ CI ] , 1.13 - 3.00 ; P=.01 ) , as was a low chronotropic index ( adjusted RR , 2.19 ; 95 % CI , 1.43 - 3.44 ; P<.001 ) . - pubmed
RESULTS : In the open trial , after 4 weeks ' treatment with HpPro 7.5 mg orally three times daily , acute hepatitis , chronic hepatitis and fatty liver cases showed rapid decrease of SGOT and SGPT . - pubmed
Low - dose fosinopril led to 70 % reduction in urinary albumin excretion ( UAE ) ( 220 + /- 199 vs 82 + /- 67 mg/24 - h , p & lt ; 0.05 ) , but UAE did not change during placebo treatment ; the maximal change became apparent after 3 months and was reversible once treatment was stopped . - pubmed
The EXT1 and EXT2 genes encode glycosyltransferases involved in biosynthesis of heparan sulphate proteoglycans . - pubmed
The purpose of the present work is to further modify the previously developed headspace ( HS ) GC - MS method for blood isoflurane determination to analyze and distinguish five common clinical inhalation anesthetics , simultaneously . - pubmed
, 0.25 microm film thickness DB-5 capillary column along with an adequate GC temperature program , which gives the five inhalation anesthetics , including isoflurane and its isomer , enflurane , a high resolution . - pubmed
Importance of timing of post - contrast MRI in rheumatoid arthritis : what happens during the first 60 minutes after IV gadolinium - DTPA ? - pubmed
OBJECTIVE : To determine the time period after IV MRI contrast ( gadolinium - DTPA ( Gd ) ) injection in which synovial membrane volume determination is reliable . - pubmed
METHODS : MRI of five RA knees with clinical synovitis was carried out , with axial , T(1 ) weighted , spin echo images before IV Gd injection and every 1.75 minutes for 60 minutes post - Gd . - pubmed
Compared with volumes at all other time points , synovial membrane volumes at 0.75 and 2.50 minutes post - Gd were significantly lower ( Wilcoxon - Pratt ) , suggesting that some synovial membrane areas had not yet exceeded the enhancement threshold . - pubmed
Mutant acyl - coenzyme A : cholesterol acyltransferase 1 devoid of cysteine residues remains catalytically active . - pubmed
Acyl - coenzyme A : cholesterol acyltransferase ( ACAT ) plays important roles in cellular cholesterol homeostasis and in the early stages of atherosclerosis . - pubmed
We constructed various ACAT1 mutants that contained either single cysteine to alanine substitution at various positions , contained a reduced number of cysteines , or contained no cysteine at all . - pubmed
Therefore , cysteine is not essential for ACAT catalysis . - pubmed
The availability of the cysteine - free ACAT1 will facilitate future ACAT structure function studies . - pubmed
Additional studies show that Cys467 is one of the major target sites that leads to p - chloromercuribenzene sulfonic acid - mediated ACAT1 inactivation , suggesting that Cys467 may be near the ACAT active site(s ) . - pubmed
Male fertility is compatible with an Arg(840)Cys substitution in the AR in a large Chinese family affected with divergent phenotypes of AR insensitivity syndrome . - pubmed
We reported here an Arg(840)Cys substitution in the AR gene in a large Chinese pedigree affected with AIS . - pubmed
We now identify a second site in A1 at Lys(36 ) that is cleaved by factor Xa . - pubmed
These affinity values were consistent with values obtained by fluorescence energy transfer using acrylodan - labeled A2 and fluorescein - labeled A1 . - pubmed
This resulted in part from a 5 - fold increase in K(m ) for factor X when A1 was cleaved at Arg(336 ) . - pubmed
Among the enzymes that regulate dopamine turnover , tyrosine hydroxylase ( TH ) , the rate - limiting anabolic enzyme , could be a target for endocrine feedback regulation . - pubmed
We demonstrated that preoptic TH and estradiol receptor mRNA levels are greatly decreased by gonadectomy during vitellogenesis . - pubmed
For TH expression , this effect was reversed in part by estradiol supplementation . - pubmed
We have also confirmed the existence of an inhibitory gonadal feedback on FSH secretion , mediated by estradiol . - pubmed
The stimulating effect of estradiol on TH expression found in this study could be a pathway involved in gonadal feedback on gonadotropin release . - pubmed
Analysis of 7 - HPG in DNA by gas chromatography - high - resolution mass spectrometry ( GC - HRMS ) showed a linear response in 7 - HPG for all three tissues after 3 days of exposure , and for NRE and lung after 20 days of exposure . - pubmed
PURPOSE : To study the kinetics of polylactide ( PLA ) nanoparticle ( NP ) localization within the intraocular tissues and to evaluate their potential to release encapsulated material . - pubmed
METHODS : A single intravitreous injection ( 5 micro L ) of an NP suspension ( 2.2 mg / mL ) encapsulating either Rh-6 G ( Rh ) or Nile red ( Nr ) was performed . - pubmed
PLA NPs were still present within the RPE cells 4 months after a single intravitreous injection . - pubmed
CONCLUSIONS : Intravitreous injection of PLA NPs appears to result in transretinal movement , with a preferential localization in the RPE cells . - pubmed
The inhibition of porphobilinogen deaminase by isoporphobilinogen . - pubmed
Subjects with a shortened activated partial thromboplastin time show increased in - hospital mortality associated with elevated D - dimer , C - reactive protein and glucose levels . - pubmed
D - dimer , C - reactive protein ( CRP ) and glucose , markers that have been related with increased mortality , were tested . - pubmed
Non - survivors with short aPTTs had significantly higher plasma D - dimer , CRP and glucose levels compared with survivors . - pubmed
By using truncated versions of HRGP , we demonstrate that the isolated 150 amino acid - residue His / Pro - rich domain , which is also released by spontaneous proteolysis from purified HRGP , mediates the inhibitory effect on chemotaxis . - pubmed
Moreover , the His / Pro - rich domain must be released from HRGP to exert its effect . - pubmed
Our working hypotheses were : ( 1 ) liver expresses considerable SSADH activity and therefore represents a major source of GHB output , ( 2 ) correction of liver enzyme deficiency will reduce GHB load both peripherally and in the central nervous system , and ( 3 ) SSADH expression will improve survival . - pubmed
Molecular aging of tau : disulfide - independent aggregation and non - enzymatic degradation in vitro and in vivo . - pubmed
Most importantly , tau was spontaneously degraded , generating fragments that start from bulky residues next to asparaginyl residues . - pubmed
This spontaneous degradation of tau probably represents non - enzymatic cleavage through the formation of succinimide intermediates . - pubmed
Similar degradation products starting from the bulky residues next to asparaginyl residues were found in the smeared tau in vivo partially purified from the homogenates from Alzheimer ' s disease brains . - pubmed
Inhibition with merthiolate of the mucooligosaccharase fraction of testis hyaluronidase ( mesomucinase ) . - pubmed
Interestingly , among all the studied P450 enzymes , only CYP2D1 was not affected by NO donors . - pubmed
In the present study , we systematically evaluated the effects of NO on the enzyme kinetic parameters of marker substrates for a range of P450 enzymes , including 2D1 . - pubmed
These results indicate that NO can potentially change both the Vmax and Km of various substrates selectively and confirm our previous findings that the activity of CYP2D1 is not affected by NO donors . - pubmed
Adrenalectomy per se had no effect on the concentration of LH , FSH , or testosterone or on the weights of the prostate or seminal vesicles . - pubmed
AIM : To investigate the release of cyclodextrin-5 - aminosalicylic acid ( CyD-5 - ASA ) in cecum and colon . - pubmed
The observation that the Q(e ) peak precedes the Q(q ) decline would be consistent with the decreases in Calvin cycle carbon flow occurring at the kinase reactions prior to glyceraldehyde-3 - phosphate dehydrogenase . - pubmed
The isolation of asparagine from an enzymic digest of edestin . - pubmed
Platelet function was measured immediately at admission , and aspirin resistance was defined as a collagen / epinephrine Closure Time ( CT(CEPI))<165 s. RESULTS : We found that 70 ( 23.5 % ) patients were aspirin resistant , and 70 ( 23.5 % ) patients ended up with the diagnosis of an AMI . - pubmed
The most common types of barrier membranes used for bone or tissue regeneration are made of expanded - polytetrafluoroethylene ( e - PTFE ) or resorbable materials , such as collagen . - pubmed
Calcium - sensitive regulation of monoamine oxidase - A contributes to the production of peroxyradicals in hippocampal cultures : implications for Alzheimer disease - related pathology . - pubmed
BACKGROUND : Calcium ( Ca2 + ) has recently been shown to selectively increase the activity of monoamine oxidase - A ( MAO - A ) , a mitochondria - bound enzyme that generates peroxyradicals as a natural by - product of the deamination of neurotransmitters such as serotonin . - pubmed
It has also been suggested that increased intracellular free Ca2 + levels as well as MAO - A may be contributing to the oxidative stress associated with Alzheimer disease ( AD ) . - pubmed
The effects of A23187 and CB-28 K are both independent of any change in MAO - A protein or gene expression . - pubmed
CONCLUSION : These data suggest that increases in intracellular Ca2 + availability could contribute to a MAO - A - mediated mechanism with a role in AD - related oxidative stress . - pubmed
On the other hand , hepatic CYP7A1 activity , fecal dry weight , and fecal excretion of bile acids increased linearly with increasing DF , while body weight gain decreased . - pubmed
The complex is insensitive to acute rapamycin exposure and has shown functions in controlling cell growth and actin cytoskeletal assembly . - pubmed
It has been suggested that dimethylsulfoxide ( DMSO ) acts via the oxytocin / oxytocin receptor pathway because an oxytocin receptor antagonist not only blocks oxytocin - induced cardiomyocyte differentiation , but also blocks DMSO - induced differentiation . - pubmed
Monoterpene cineole , pentacyclic triterpenes and steroids were identified by TLC and GC - FID . - pubmed
These findings now place PKCtheta as a key signaling molecule in the positive selection of thymocytes as well as in the activation of mature T cells . - pubmed
Mutants of hsPrP(84 - 114 ) with two or one histidyl residues have also been synthesized and their copper ( II ) complexes studied by CD spectroscopy . - pubmed
Each individual contiguous network of Ni , YSZ , and porosity was identified and labeled according to whether it was contiguous with the rest of the electrode . - pubmed
In SSc , the presence of anti - S cerevisiae alpha - enolase antibodies was associated with interstitial lung disease ( ILD ) , decreased total lung capacity ( 73.2 % vs 89.7 % ; p<0.001 ) and diffusion capacity for carbon monoxide ( 47.4 % vs 62.3 % ; p<0.001 ) , and antitopoisomerase 1 antibodies ( 46 % vs 21 % ; p = 0.005 ) but not anticentromere antibodies ( 11 % vs 34 % ; p = 0.006 ) . - pubmed
Here , we focus on the origin and migratory routs of prospective CB(1)/cholecystokinin ( CCK)+ and CB(1)/reelin / calretinin+ gamma - aminobutyric acid ( GABA)-ergic hippocampal interneurons . - pubmed
METHODS : We prepared cationic NP encapsulated with a fluorescence marker ( FITC ) by emulsion solvent diffusion method , succeeded to formulate an NP - eluting stent with a novel cation electrodeposition coating technology , and compared the in vitro and in vivo characteristics of the FITC - loaded NP - eluting stent with dip - coated FITC - eluting stent and bare metal stent . - pubmed
These criteria are evaluated with respect to the confirmation of cocaine and its metabolites by single quadrupole liquid chromatography / mass spectrometry ( LC / MS ) and problems are highlighted . - pubmed
Spiked samples , proficiency testing samples , certified reference materials and samples from real cases that had screened positive for cocaine derivatives by immunoassay were subjected to confirmation by LC / MS using single ion monitoring with in - source fragmentation . - pubmed
Decalcified sections were examined by hematoxylin and eosin ( HE ) staining , alkaline phosphatase ( ALPase ) immunohistochemistry and tartrate - resistant acid phosphatase ( TRAPase ) enzyme histochemistry . - pubmed
MS / MS detection was performed by monitoring the parent-->daughter pair of levosulpiride and the IS at m / z 342 - ->112 and 329 - ->256 , respectively . - pubmed
[ A study of the catalytic properties of the nitrile hydratase immobilized on aluminum oxides and carbon - containing adsorbents ] . - pubmed
The activity and operational stability of the immobilized nitrile hydratase were studied in the reaction of acrylonitrile transformation into acrylamide . - pubmed
It was demonstrated that an increase in the carbon content in the carrier led to an increase in the amount of adsorbed enzyme and , concurrently , to a decrease in its activity . - pubmed
The nitrile hydratase immobilized on Sibunit and carbon - containing aluminum alpha - oxide having a " crust " structure displayed the highest operational stability in acrylonitrile hydration . - pubmed
As a result , ten compounds were purified and three of them were identified as 3 - methyl-5 - vinyloxazolidin-2 - one ( compound A ) , 5 - hydroxymethyl-2 - furaldehyde ( compound B ) and 3 - methylfuran-2 - carboxylic acid ( compound G ) based on the MS , IR and extensive NMR data , respectively . - pubmed
The aggregation of solutes occurs due to a specific H (+ ) pump denominated V - ATPase that maintains an inner acidic media ( pH 5.5 ) . - pubmed
When this gradient is reduced by modulation of the V - ATPase activity , catecholamines and Ca ( 2 + ) are moved toward the cytosol . - pubmed
Jack bean alpha - mannosidase digestion profile of hybrid - type N - glycans : effect of reaction pH on substrate preference . - pubmed
In this study , the JBM digestion profile of hybrid - type N - glycans was examined using pyridylamino ( PA - ) sugar chains . - pubmed
The digestion efficiencies of the PA - labeled hybrid - type N - glycans Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6 ( GlcNAcbeta1,2Manalpha1,3 ) Manbeta1,4GlcNAcbeta1,4GlcNAc - PA ( GNM5 - PA ) and Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6 ( Galbeta1,4GlcNAcbeta1,2Manalpha1,3 ) Manbeta1,4GlcNAcbeta1,4GlcNAc - PA ( GalGNM5 - PA ) were significantly lower than that of the oligomannose - type N - glycan Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6Manbeta1,4GlcNAcbeta1,4GlcNAc - PA ( M4 - PA ) , and the trimming pathways of GNM5 - PA and GalGNM5 - PA were different from that of M4 - PA , suggesting a steric hindrance to the JBM activity caused by GlcNAcbeta1 - 2Man ( alpha ) residues of the hybrid - type N - glycans . - pubmed
The ERalpha selective agonist , 4,4 ' , 4 ''-[ 4 - propyl -( 1H )- pyrazole-1,3,5 - triyl ] tris - phenol ( PPT ; s.c . - pubmed
The current evaluation of glucuronide metabolites of BUP and norBUP are suggestive of combined inhibition of Uridine diphosphate ( UDP )- glucuronosyltransferase of the 1A family and cytochrome P450 3A4 that spares UGT2B7 leading to a shunting of BUP away from production of norBUP and toward BUP-3 G as seen by a statistically significant increase in the AUC of BUP-3G. - pubmed
Using atomic force microscopy on living cells , we show that septin depletion significantly reduced the unbinding force of InlB - Met interaction and the viscosity of membrane tethers at locations where the InlB - Met interaction occurs . - pubmed
Most available studies have addressed steroid and calcineurin inhibitor minimization in an attempt to prevent the development of chronic allograft dysfunction and cardiovascular mortality . - pubmed
It is known that TSH binding to the TSHR depends on the interaction with the leucine - rich repeat and sulfation at Y385 of the hinge region . - pubmed
Indeed , we identified further amino acids of the hinge region with influence on bTSH binding . - pubmed
Moreover , side chain variations revealed that different amino acid properties like the negative charge , aromatic as well as hydrophilic characteristics , contribute to maintain the hormone - TSHR hinge interaction . - pubmed
BACKGROUND : Multiple sclerosis ( MS ) is associated with pathogenic autoimmunity primarily focused on major CNS - myelin target antigens including myelin basic protein ( MBP ) , proteolipidprotein ( PLP ) , myelin oligodendrocyte protein ( MOG ) . - pubmed
This , together with previously demonstrated disease - susceptibility to MBP and MOG in DRB1 * 1501 - transgenics , also suggests a differential role for DRB1 * 1501 and DQB1 * 0602 depending on target antigen and imply a potential complex ' genotype / target antigen / phenotype ' relationship in MS heterogeneity . - pubmed
Stable - isotope dilution GC - MS method for ethanol in vapour ethanol and microdialysis systems based on carbonate - catalyzed extractive pentafluorobenzoylation . - pubmed
We present a novel GC - MS method for the accurate and precise analysis of ethanol in cell cultures and microdialysates . - pubmed
The method is based on the carbonate - catalyzed extractive pentafluorobenzoylation of ethanol and deuterium - labelled ethanol serving as the internal standard and on their GC - MS analysis in the electron - capture negative - ion chemical ionization mode . - pubmed
All patients had the same , novel homozygous c.1580G>T LMNA mutation , resulting in the replacement of arginine 527 by leucine . - pubmed
Akt phosphorylates HK - II at Thr-473 and increases mitochondrial HK - II association to protect cardiomyocytes . - pubmed
Expression of an HK - II - dissociating peptide diminished IGF-1 - induced increases in mitochondrial HK - II as well as protection against hydrogen peroxide treatment , suggesting an important role of mitochondrial HK - II in IGF-1 / Akt - mediated protection . - pubmed
We hypothesized , on the basis of an Akt phosphorylation consensus sequence present in HK - II , that Thr-473 is the target of Akt kinase activity . - pubmed
Indeed , recombinant kinase - active Akt robustly phosphorylates WT HK - II , but not Thr-473 mutants . - pubmed
Phosphomimetic ( T473D)HK - II , but not non - phosphorylatable ( T473A)HK - II , constitutively increased mitochondrial binding compared with WT HK - II and concomitantly confers greater protection against hydrogen peroxide . - pubmed
Glucose 6 - phosphate ( G-6P ) , a product of the catalytic activity of HK - II , is well known to dissociate HK - II from mitochondria . - pubmed
Addition of G-6P to isolated mitochondria dose - dependently dissociates WT HK - II , and this response is inhibited significantly in mitochondria isolated from cardiomyocytes expressing T473D HK - II . - pubmed
Pretreatment with IGF-1 also inhibits G-6P - induced overexpressed or endogenous HK - II dissociation , and this response was blocked by Akt inhibition . - pubmed
These results show that Akt phosphorylation of HK - II at Thr-473 is responsible for the Akt - mediated increase in HK - II binding to mitochondria . - pubmed
Tyrosinase inhibition activity was investigated using the dopachrome method with L-3,4 - dihydroxyphenylalanine ( L - DOPA ) as a substrate . - pubmed
The elastase inhibition activities of ethanol ( 250 mug / ml ) and ethyl acetate ( 125 mug / ml ) extracts were found to be significantly higher than that of the negative control . - pubmed
The tyrosinase inhibition activities of ethanol and ethyl acetate extracts , in terms of the mean inhibition concentration ( IC50 ) , were 1,078 and 1,467 mug / ml , respectively . - pubmed
Substitution of MOMP Thr(268 ) led to significantly reduced binding to BgAgs . - pubmed
The O - glycan moiety was characterized as Gal ( beta1 - 3 )- GalNAc ( beta1 - 4 )- GalNAc ( beta1 - 4 )- GalNAcalpha1 - Thr(268 ) ; modelling suggested that O - glycosylation has a notable effect on the conformation of MOMP and this modulates BgAg - binding capacity . - pubmed
Increases in reactive oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the transient receptor potential melastatin 4 ion channel . - pubmed
The transient receptor potential melastatin 4 ( TRPM4 ) protein is a ROS - modulated non - selective cationic channel that performs several cell functions , including regulating intracellular Ca ( 2 + ) overload and Ca ( 2 + ) oscillation . - pubmed
Notably , we demonstrate that the TRPM4 ion channel is critical in promoting H2O2 - enhanced EC migration . - pubmed
A post - hoc analysis using automated analysis software revealed a significant reduction in crying symptoms in the PPI + LLP group ( 99 [ 65 - 103 ] to 62 [ 32 - 96 ] episodes , P = 0.018 ) . - pubmed
Fracture resistance of endodontically treated premolars restored with lithium disilicate CAD / CAM crowns or onlays and luted with two luting agents . - pubmed
A new ring expansion of 2 - aminobenzothiazoles with alkynyl carboxylic acids was developed , which allows for one - pot synthesis of 1,4 - benzothiazines in moderate to excellent yields . - pubmed
In conclusion , warfarin treatment where patients spend a high proportion of time in the therapeutic range is safe and effective , and will continue to be a valid treatment option in the era of newer oral anticoagulants . - pubmed
TC , HDL - C , LDL - C and TAG were measured , and 2,2 '- azinobis -( 3 - ethylbenzothiazoline-6 - sulfonic acid ) radical cation ( ABTS ( + ) ) scavenging capacity and ferric reducing antioxidant power ( FRAP ) were determined in plasma . - pubmed
NADPH oxidase ( NOX ) was also measured by lucigenin - derived chemiluminiscence . - pubmed
Onion ingredient improved antioxidant status in HCO group , as it was evidenced by ABTS ( + ) and FRAP values and SOD and GPx enzyme activities compared to the HC - fed group , reduced the increment in NOX activity and reversed endothelial dysfunction promoted by the HC diet . - pubmed
MAIN OUTCOME MEASURES : Longitudinal measurements of AMH , FSH , LH , estradiol , and inhibin B according to age , karyotype ( 45,X ; 45,X/46,XX mosaicism ; miscellaneous karyotypes ) , and ovarian status ( group 0 , prepubertal ; group 1 , never ovarian function ; group 2 , ongoing ovarian function ; and group 3 , loss of ovarian function ) . - pubmed
After 14 days , the levels of nitric oxide ( NO ) , nitric oxide synthase ( NOS ) , tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1beta ( IL-1beta ) , interleukin-10 ( IL-10 ) and cyclic guanosine monophosphate ( cGMP ) in the spinal dorsal horn were measured by the corresponding kits , mRNA expression of inducible NOS ( iNOS ) and protein kinase G type I ( PKGI ) of spinal cord were analyzed by reverse - transcription polymerase chain reaction ( RT - PCR ) . - pubmed
Treatment with IGLR ( 200 , 400 , 800mg / kg ) significantly alleviated SNI - induced mechanical allodynia , markedly decreased the levels of NO , NOS , TNF - alpha , IL-1beta and cGMP , and increased the level of IL-10 . - pubmed
Meanwhile , IGLR ( 200 , 400 , 800mg / kg ) also inhibited the protein expression of NMDAR , PKCgamma and the mRNA expression of iNOS and PKGIota in the spinal cord . - pubmed
CONCLUSIONS : These results indicated IGLR could produce an anti - neuropathic pain effect that might partly be related to the inhibition of the NO / cGMP / PKG and NMDAR / PKC pathways and the level of TNF - alpha , IL-1beta as well as to the increase of the level of IL-10 in spinal cord . - pubmed
At 1 year , high - density lipoprotein cholesterol ( HDL ) and triglycerides ( TG ) improved similarly with all surgeries . - pubmed
In contrast , the accessory elements on epsilon - II plasmids were typically located between trfA and oriV , and contained IS1071 , which was commonly inserted within the Tn501 - like transposon , typically harboring a cluster of genes encoding mercury resistance and/or catabolic pathways . - pubmed
These activities have been attributed to its modulation of various molecular targets such as cyclins , apoptosis- related proteins , proteasome activity , reactive oxygen species , as well as NF - kB , AKT / mTOR and MAPK / ERK pathways . - pubmed
Ibrutinib is a new - targeted therapy that irreversibly and specifically inhibits the Bruton ' s Tyrosine Kinase ( BTK ) , a key component of the signaling pathways of B cells . - pubmed
The loss of DNA associated with unwinding joint molecules previously observed in the absence of RuvAB and RecG , is suppressed by a helicase deficient PriA mutation ( priA300 ) , arguing that the action of RecG ensures that PriA is bound correctly on D - loops to direct DNA replication rather than to unwind joint molecules . - pubmed
CONCLUSIONS : TAE plus vincristine can rapidly improve levels of platelets and fibrinogen , and it is an effective method for treatment of corticosteroid - resistant vascular tumors associated with Kasabach - Merritt phenomenon in infants . - pubmed
The PL of MoSe2 occurs in the near - infrared spectral range where the emissive properties do not suffer from the enhanced dissipation in the gold due to inter - band transitions . - pubmed
Here , we study the interaction between monolayer MoSe2 and plasmonic dipolar antennas in resonance with the PL emission of MoSe2 . - pubmed
By varying the thickness of the spacer between the MoSe2 layer and nanoantenna , we demonstrate manipulation of the PL intensity from nearly fourfold quenching to approximately threefold enhancement . - pubmed
The addition of MPG - medicated serum to the media of cultured GCs also reduced cadmium chloride - induced apoptosis and downregulated caspase-3 protein expression . - pubmed
CONCLUSION : 20 micromol / L XAV-939 and 20 mmol / L LiCl as effective inhabitor and activator of Wnt signalling pathway respectively can significantly down- and up - regulate the expression of Wnt down - stream pathway target genes and proteins . - pubmed
, erosion , diffusion , and water - mediated processes ) were studied in four release media : PBST pH 7.4 ( standard condition ) , PBST pH 6.5 , PBS+1.0 % triethyl citrate ( TC ) , and HBST pH 7.4 . - pubmed
Aspirin plus sorafenib potentiates cisplatin cytotoxicity in resistant head and neck cancer cells through xCT inhibition . - pubmed
The nonsteroidal anti - inflammatory drug aspirin and the multikinase inhibitor sorafenib have both shown experimental and clinical anticancer activities . - pubmed
The combination of aspirin and sorafenib induced xCT inhibition , GSH depletion , and ROS accumulation in cancer cells . - pubmed
Genetic and pharmacological inhibition of xCT potentiated the cytotoxic effects of aspirin plus sorafenib ; this effect was diminished by xCT overexpression . - pubmed
Uric acid levels significantly rose with body mass index ( BMI ) , waist circumference ( WC ) , triglyceride ( TG ) levels , triglycerides to high - density lipoprotein cholesterol ratio ( TG / HDL - C ) , insulin levels , homeostatic model assessment index ( HOMA - IR ) , high - sensitivity CRP ( hs - CRP ) levels , and leptin levels . - pubmed
Conversely , the levels of HDL - C and adiponectin were negatively correlated with uric acid levels . - pubmed
In multiple regression analysis , there were age , BMI , TG / HDL - C ratio , and adiponectin levels remained significantly associated with uric acid levels . - pubmed
Our findings suggest that the anti - inflammatory action of DXM via inactivation of the TLR-4 / NF - kappaB pathway , in part , may explain DXM - induced neuroprotection after cerebral ischemia . - pubmed
Here , we demonstrate that insulin temporally induces Tnfaip8 , which mediates the anti - autophagic action of insulin through formation of a novel ternary complex including Tnfaip8 , phosphatidylethanolamine ( PE ) and Galphai3 . - pubmed
Intestinal lymphatic HDL miR-223 and ApoA - I are reduced during insulin resistance and restored with niacin . - pubmed
The intestine is involved in whole - body lipid and cholesterol homeostasis and secretes lipoproteins containing apolipoprotein ( Apo ) B48 and discrete ApoA - I into the mesenteric lymph . - pubmed
The lymphatic system has been proposed to have a significant role in the reverse cholesterol transport pathway associated with HDL - ApoA - I. - pubmed
Niacin ( nicotinic acid ; vitamin B3 ) is known to regulate lipid metabolism , but the role of niacin in modulating intestinal lipid and lipoprotein ( ApoB48 and ApoA - I ) metabolism is unknown . - pubmed
Intestinal lymph HDL - ApoA - I and miR-223 expression were lower by at least 45 and 60 % , respectively , and lymph HDL was associated with 85 % higher triglyceride ( TG ) content in IR compared to non - IR control group . - pubmed
Niacin was found to increase secretion of lymph HDL and miR-223 by at least 50 - 60 % and to deplete the TGs associated with HDL compared with the nontreated IR group . - pubmed
Niacin significantly increased peroxisome proliferator - activating nuclear receptor alpha and carnitine palmitoyltransferase I alpha mRNA and annulled Tnf - alpha mRNA expression in intestinal ( jejunal ) explants . - pubmed
Altered intestinal lymphatic HDL - ApoA - I and miR-223 metabolism in IR and modulation by niacin may provide insight into the intestinal - mediated regulation of the reverse cholesterol transport pathway.-Mangat , R. , Borthwick , F. , Haase , T. , Jacome , M. , Nelson , R. , Kontush , A. , Vine , D. F. , Proctor , S. D. Intestinal lymphatic HDL miR-223 and ApoA - I are reduced during insulin resistance and restored with niacin . - pubmed
However , the CRP rs1130864 A allele carrier state was significantly more frequent : ( i ) in BD patients with thyroid disorders than in those without ( pc = 0.046 ) , especially among females ( pc = 0.01 ) and independently of lithium treatment , ( ii ) in BD patients with rapid cycling than in those without ( pc = 0.004 ) . - pubmed
BCS is caused by hypercoagulability , e.g , arising from malignancy , oral contraceptives , and deficiency of protein S or C. It is not rare that BCS often shows venous thrombosis , including in superior vena cava . - pubmed
When the SRCHA was initiated by the trigger DNA , a series of DNA assembly steps based on the toehold - mediated DNA strand displacement were activated , and the Y shaped DNA ( P1 - P2 - P3 ) was formed . - pubmed
High parathyroid hormone levels ( PTH ) were associated with early - phase Tc99m - sestamibi positivity in both primary ( p = 0.01 ) and secondary hyperparathyroidism ( p = 0.03 ) but not with last phase ( p = 0.11 , p = 0.37 , respectively ) . - pubmed
CONCLUSION : Pre - operative PTH levels and size of the gland were major determinants of Tc99m - sestamibi positivity on early - phase Tc99m - sestamibi scans , whereas size is an independent predictor of late - phase Tc99m - sestamibi positivity . - pubmed
Quantification of U-47700 and its metabolites in plasma by LC - MS / MS . - pubmed
Serum MDA , SOD , and GSH - Px levels were detected . - pubmed
CPE gavage obviously reduced FBG level , increased body weight , enhanced glucose tolerance , elevated FINS level and ISI , and reduced IRI , all in a dose - dependent manner . - pubmed
The cholinesterase inhibitor , donepezil ( Aricept ) , reverses AIE effects on dendritic spines , possibly by interacting with inflammatory and/or epigenetic mediators after AIE exposure . - pubmed
All of these AIE effects were also reversed by donepezil , with the exception of HMGB1 . - pubmed
In the present study , the roles of AITC in up - regulating the MRP1 expression and its relationship with the activation of the Notch1 signaling pathway were investigated by combining the in vivo and in vitro experiments . - pubmed
In contrast to the COPD model group , the expressions of MRP1 , Hes1 and Notch1 dramatically up - regulated following the treatment with Low / High doses of AITC . - pubmed
The expression of MRP1 in the 16 HBE cells was down - regulated by the inhibition of Notch in a DAPT concentration - dependent manner . - pubmed
Additionally , the AITC - induced up - regulation of the MRP1 expression was markedly impaired following the inhibition of Notch1 . - pubmed
Biocompatible poly - epsilon - caprolactone ( PCL ) , PCL / silk fibroin ( SF ) and PCL / SF / Au(SiO2 ) loaded nanofibrous scaffolds were first fabricated by an electrospinning method . - pubmed
Osteogenic differentiation of MSCs into osteogenic cells were determined by alkaline phosphatase ( ALP ) activity , mineralization by alizarin red S ( ARS ) staining and osteocalcin expression by immunofluorescence staining . - pubmed
The results revealed that the addition of SF and Au(SiO2 ) to PCL scaffolds enhanced the mechanical strength , interconnecting porous structure and surface roughness of the scaffolds . - pubmed
alpha - Linolenic acid but not linolenic acid protects against hypertension : critical role of SIRT3 and autophagic flux . - pubmed
In addition , SHRs displayed reduced vascular Sirtuin 3 ( SIRT3 ) expression , subsequent superoxide dismutase 2 ( SOD2 ) hyperacetylation and mitochondrial ROS overproduction , all of which were ameliorated by ALA but not LA supplementation . - pubmed
In primary cultured endothelial cells , ALA treatment directly inhibited SIRT3 reduction , SOD2 hyperacetylation , mitochondrial ROS overproduction and alleviated autophagic flux impairment induced by AngII plus TNFalpha treatment . - pubmed
However , these beneficial effects of ALA were completely blocked by silencing SIRT3 . - pubmed
Restoration of autophagic flux by rapamycin also inhibited mitochondrial ROS overproduction in endothelial cells exposed to AngII plus TNFalpha . - pubmed
Our findings suggest that ALA but not LA supplementation improves endothelial dysfunction and diminishes experimental hypertension by rescuing SIRT3 impairment to restore autophagic flux and mitochondrial redox balance in endothelial cells . - pubmed
After RNA extraction with precipitation method , the Syber green qReal - time Polymerase Chain Reaction ( PCR ) was performed in order to evaluate the possible alteration in the expression of IL-6 , BCL-2 , miR-181b-5p , and miR-23a-3p . - pubmed
PLOD2 increases resistance of gastric cancer cells to 5 - fluorouracil by upregulating BCRP and inhibiting apoptosis . - pubmed
The present study aimed to determine whether PLOD2 could confer 5 - FU resistance in GC . - pubmed
Our mechanistic studies revealed that PLOD2 - overexpressing cells appear to be resistant to the therapeutic characteristics of 5 - FU in GC cells by upregulating BCRP and that PLOD2 confers resistance to 5 - FU - induced apoptosis in GC cells by affecting the expression of Bax and Bcl2 . - pubmed
Conclusion : PLOD2 contributed to increasing resistance of gastric cancer cells to 5 - fluorouracil by upregulating BCRP and inhibiting apoptosis . - pubmed
As a NAD+-dependent deacetylase , SIRT1 is widely involved in apoptosis and cellular inflammation via multiple pathways such as p53 , NF - kB and STAT . - pubmed
The AVPI - bearing peptide assemblies in the cytosol sequestrate IAPs and sensitize the cancer cells to bortezomib ( BTZ ) , a proteasome inhibitor , but rescue the normal cells ( i.e. - pubmed
Alkaline phosphatase ( ALP ) acts as a context - dependent signal for trafficking the peptide / BTZ assemblies and selectively inducing the death of the cancer cells . - pubmed
Adropin regulates hepatic glucose production via PP2A / AMPK pathway in insulin - resistant hepatocytes . - pubmed
Mechanistically , adropin dissociated CREB - CRTC2 and FoxO1 - PGC1alpha complex and reduced their binding to the promoters of G6Pase and PEPCK to decrease glucose production in IR . - pubmed
This study provides evidence that adropin activates the AMPK pathway via inhibition of PP2A and decreases the liver glucose production in IR context . - pubmed
Two clones with higher - level resistance had the mutation pattern of Asn87Lys and one lower - level LVX - resistant clone had an Asp91Asn mutation . - pubmed
Increase in the number of SNVs of the Hp0970 ( hefE ) and Hp1329 ( hefI ) genes change the resistance to LVX . - pubmed
Twelve mutations verified by Sanger sequencing in Hp0970 ( hefE ) and Hp1329 ( hefI ) may decrease resistant levels to LVX . - pubmed
Acetylcholine muscarinic receptors ( mAChRs ) contribute to both the facilitation and inhibition of levodopa - induced dyskinesia operated by striatal cholinergic interneurons , although the receptor subtypes involved remain elusive . - pubmed
Cholinergic afferents from the midbrain also innervate the substantia nigra reticulata , although the role of nigral mAChRs in levodopa - induced dyskinesia is unknown . - pubmed
Reverse microdialysis allowed to deliver the mAChR antagonists telenzepine ( M1 subtype preferring ) , PD-102807 and tropicamide ( M4 subtype preferring ) , as well as the selective M4 mAChR positive allosteric modulator VU0152100 in striatum or substantia nigra , while levodopa was administered systemically . - pubmed
The M2 subtype preferring antagonist AFDX-116 , used to elevate striatal acetylcholine levels , blocked the behavioral and neurochemical effects of PD-102807 . - pubmed
In accordance with Article 6 of Regulation ( EC ) No 396/2005 , the applicants Bayer CropScience AG and Bayer SAS submitted two requests to the competent national authority in the Netherlands to set import tolerances and to modify existing EU maximum residue levels ( MRLs ) for the active substance flupyradifurone and its metabolite difluoroacetic acid ( DFA ) in various crops . - pubmed
In general , the safety profile of tofacitinib is consistent with that of biologic disease - modifying anti - rheumatic drugs , with an increased risk of herpes zoster that seems to be a class effect of Janus kinase inhibitors . - pubmed
Rethinking the Citric Acid Cycle : Connecting Pyruvate Carboxylase and Citrate Synthase to the Flow of Energy and Material . - pubmed
Here , we add the heart lactate dehydrogenase proton - linked monocarboxylate transporter 1 complex , connecting the original Citric Acid Cycle to the flow of energy and material . - pubmed
The heart lactate dehydrogenase proton - linked monocarboxylate transporter 1 complex catalyses the first reaction of the Citric Acid Cycle , the oxidation of lactate to pyruvate , and thus secures the provision of pyruvic acid . - pubmed
Cycad Genotoxin Methylazoxymethanol Disrupts the Brain Ubiquitin - Proteasome Pathway , Tau and alpha - Synuclein , as Reported in ALS - PDC . - pubmed
These results provide in vivo evidence in support of the hypothesis that adenosine caused inhibition of sympathetic neurotransmission via an action on purinergic receptors located on sympathetic nerve terminals . - pubmed
These data suggest that fluorescence is generated only through the coupling of fluorescamine to the N - terminal primary amine of ATII . - pubmed
The rate loss of biological activity of the ATII - fluorescamine derivative was appreciably lower than that observed for ATII . - pubmed
The present study suggests that the ATII - fluorescamine derivative can be substituted for radioactively - labelled ATII for use in a variety of applications . - pubmed
Half of the subjects received 400 gamma of fenoterol ( beta2 - sympathicomimetic ) , the other ones 200 gamma of SCH 1000 ( synthetic atropine ) . - pubmed
Cross - linking of troponin with dimethylimido esters . - pubmed
The topology of troponin , the calcium binding regulatory protein in muscle , has been studied by cross - linking with different length dimethylimido esters . - pubmed
Cross - linked troponin has lost the ability to make the actomyosin ATPase calcium sensitive although it does bind to actin - tropomyosin and tropomyosin , and it binds calcium normally . - pubmed
Whereas there was little detectable 45Ca2 + uptake into control cells in either the absence or presence of extracellular ATP , a marked time - dependent uptake of 45Ca2 + was observed in the presence of ATP in cells fused with SR Ca - ATPase . - pubmed
The newly incorporated native kidney pumps , while of the same isoform as the endogenous red cell pump , behave differently from the endogenous red cell sodium pump with respect to their very low " uncoupled " Na+/O flux activity . - pubmed
Retinoids selective for retinoid X receptor response pathways . - pubmed
However , in the presence of 9 - cis - retinoic acid , a ligand for both RARs and RXRs , RXRs can also form homodimers . - pubmed
A series of retinoids is reported that selectively activates RXR homodimers but does not affect RAR - RXR heterodimers and thus demonstrates that both retinoid response pathways can be independently activated . - pubmed
[ Effects of neurotropin on seizure activity in picrotoxin kindling ] . - pubmed
Under conditions of acute picrotoxin -- induced seizures it was observed that preliminary cycloheximide ( protein - synthesis blocker ) administration abolished anticonvulsant properties of neurotropin . - pubmed
Body weight , hemoglobin , hematocrit , and viable cell yield of pups were significantly decreased with decreasing dietary iron . - pubmed
As previously shown , in the presence of tetrodotoxin ( 1 microM ) , acetylcholine ( 50 microM ) induces a prolonged stimulation of [ 3H]dopamine release in both compartments through an interaction with muscarinic receptors . - pubmed
Our present study indicates that both dynorphin 1 - 13 ( 1 microM ) and the selective kappa agonist trans-3,4 - dichloro - N - methyl - N [ 2 -( 1 - pyrrolidinyl ) cyclohexyl ] benzeneace tamine ( U50488 ) ( 1 microM ) inhibit the tetrodotoxin - resistant acetylcholine - evoked release of [ 3H ] dopamine , these effects being slightly more pronounced in the matrix than in the striosomal enriched area . - pubmed
These results suggest that dynorphin exerts an inhibitory presynaptic control of dopamine release through kappa opioid receptors located on dopamine nerve terminals in the striosome as well as in the matrix . - pubmed
A study was performed to examine the role of prostaglandins ( PGs ) in the mechanism of the ethanol - induced suppression of FBM , in which the objective was to test the hypothesis that fetal administration of PGE2 can suppress the incidence of FBM following reversal of ethanol - induced suppression of FBM by indomethacin , a fatty acid cyclooxygenase inhibitor . - pubmed
FBM were suppressed during the period of ethanol infusion ( 9.6 + /- 1.7 % ) ; the ethanol - induced suppression of FBM was reversed by fetal indomethacin treatment ( 77.5 + /- 14.1 % ) ; shortly after the onset of fetal PGE2 infusion , the incidence of FBM decreased to a 2 - h mean incidence of 14.1 + /- 4.2 % , which was similar in magnitude to that observed after maternal ethanol infusion . - pubmed
Serum PRL was significantly elevated through day 14 , while E2 was elevated through day 7 by E2 - CDS . - pubmed
But much more [ 125I]fibrin deposition was present in CS reactions at 24 h than at 2 or 6 h after Ag challenge . - pubmed
The system was initially stabilized at a sinus holding pressure ( HP ) of 100 mmHg and an average SAP of 107.5 mmHg . - pubmed
The distribution of HLA - A and - B types in the patients with enhanced class I expression did not relate to those antigens previously detected more frequently on erythrocytes , B7(Bga ) , B17(Bgb ) , A28(Bgc ) , B8 or A10 , and the enhancement was not associated with any particular HLA types . - pubmed
In this report activated lymphocytes are demonstrated by using an avidin - biotin - peroxidase complex ( ABC ) method combined with autoradiography that identifies DNA synthesis and , thus , activation . - pubmed
These findings agree well with the assumption that CD8 - positive cells identify the foreign antigen in the context of class I antigens , whereas CD4 - positive cells are found to be associated with the recognition of MHC locus II coded HLA antigens . - pubmed
When octylglucoside lysates of lactoperoxidase iodinated cells were applied to 80 - kDa - Sepharose columns , a polypeptide complex of 152/125 kDa was eluted with the synthetic peptide GRGDSPC , but not with GRGESP . - pubmed
The role of the thyroid gland in sterility is not well known ; hyperthyroidism may have an effect upon estrogen secretion by decreasing the level of the free forms of estradiol , resulting in a lowering or disappearance of the LH ovulatory peak . - pubmed
Feed digestibility and nitrogen economy were not markedly affected but radioisotopic measurements demonstrated an increase in albumin disappearance and catabolic rates , and plasma faecal clearance during the course of the infection . - pubmed
Helix Pomatia ( HPA ) and carcinoembryonic antigen ( CEA ) staining were studied in 113 primary breast carcinomas , 63 metastatic lymph nodes and 10 resected local recurrences , using the avidin - biotin - peroxidase complex ( ABC ) method . - pubmed
Treatment was monitored hormonally by the determinations of LH , FSH , 17 beta - estradiol , prolactin , progesterone , testosterone ( T ) ( total and free ) , androstenedione ( delta 4A ) , dehydroepiandrosterone sulfate ( DHEA - S ) , and sex hormone - binding globulin ( SHBG ) every 2 days . - pubmed
These studies suggest that lactose may have an important function in stabilization of newborn intestinal disaccharidase enzymes . - pubmed
Using rhodamine - phalloidin fluorescence microscopy , we observed that normal hepatocytes showed a submembranous F - actin distribution with focal short microfilaments extending into filopodia along the periphery of the cell . - pubmed
Hepatocytes exposed to AFB1 for several hours exhibited retraction of their cytoplasm within a prominent circumferential peripheral band of F - actin microfilament bundles . - pubmed
Both the blebbing and the polar aggregation of F - actin bundles occurred prior to cell death as shown by lactate dehydrogenase release and trypan blue exclusion . - pubmed
Calcium - dependent regulation of phosphorylase activation in a fast - twitch oxidative - glycolytic skeletal muscle . - pubmed
This system provided an opportunity to compare the regulation of contraction and activation of phosphorylase by extracellular and intracellular sources of Ca2 + . - pubmed
Blocking extracellular Ca2 + entry promoted an inactivation of phosphorylase and diminished the elevation of resting tension , which in untreated muscles ensues with the onset of fatigue . - pubmed
This appears to occur because , although Ca2 + induces phosphorylase activation , a subsequent , but rapid non - Ca2 + -mediated event promotes inactivation , even while Ca2 + -mediated contraction is being sustained . - pubmed
Differences could also be stated for distinct HLA antigen combinations comprising these relevant antigens . - pubmed
Evidence that N6 - PIA acted as a high - affinity agonist against the external adenosine receptor was provided by experiments in which 100 microM caffeine , a known antagonist , competitively blocked its effect . - pubmed
The immunoreactivity of prothrombin was approximately 40,000 times less than that of Pr2 on a molar basis . - pubmed
Biosynthesis of adrenal corticosteroids : energy metabolism and the hydroxylases . - pubmed
[ Relation between beta - lipoprotein content in serum and plasma vitamin E content . - pubmed
Carbonic anhydrase analyses of the blood of subjects exposed to a helium - oxygen environment at seven atmospheres pressure . - pubmed
[ Reduction of post - prostatectomy bleeding by medication ( experience with the antifibrinolytic agent AMCA ) ] . - pubmed
Moreover , the gonadotrope inhibition induced by LHRH agonist with prolonged action can be of therapeutic use in idiopathic precocious puberty , prostatic cancer and inhibition of spermatogenesis ( an association with androgens being necessary in this last case ) . - pubmed
Ceftriaxone was administered IV at a dose of 2 grams daily , either as a single injection of 2 g or as two injections of 1 g each . - pubmed
A comparison of a powerful luteinizing hormone releasing hormone analogue agonist and estrogen in the treatment of advanced prostatic cancer . - pubmed
Characterization of a chromatographically purified d 1.063 - 1.21 or HDL density fraction from fatty - fibrous plaques demonstrated particles of between 60 and 120 A diameter , a characteristic apolipoprotein A - I band by SDS - polyacrylamide gel electrophoresis , and a precipitin peak closely migrating with that for plasma HDL by two - dimensional immunoelectrophoresis . - pubmed
The d greater than 1.21 density fraction from plaques isolated by affinity chromatography on a Sepharose - anti - apolipoprotein A - I column contained small amounts of phospholipid but no measurable cholesterol . - pubmed
The d 1.063 - 1.21 density fraction from plaques showed a significant increase in percent free cholesterol and phospholipid contents and decrease in cholesteryl ester content relative to plasma HDL . - pubmed
This increase in free cholesterol could represent evidence for an anti - atherogenic mechanism wherein infiltrated HDL removes cholesterol together with phospholipid from the arterial wall . - pubmed
The ability of substance P ( SP ) to regulate peak benzyl - penicilloyl ( BPO)-specific IgE antibody - forming cell ( AFC ) responses in vivo and the ability of SP and other neuropeptides to regulate BPO - specific memory IgE AFC responses induced in vitro was determined . - pubmed
Inclusion of either SP or vasoactive intestinal peptide ( VIP ) , but not neurotensin , serotonin , somatostatin , or gastrin , in cultures suppressed these responses in isotype - specific , dose - dependent fashion ( approximately 70 % ) . - pubmed
Secondary steroid resistance in asthma may arise in response to the release of cytokines that activate AP-1 and other transcription factors that bind directly to GR . - pubmed
A similar effect may also be seen with high concentrations of beta 2 - agonists that activate another GR binding transcription factor , CREB . - pubmed
Sequencing of part of this fragment revealed a gene ( acmA ) that could encode a polypeptide of 437 amino acid residues . - pubmed
In AcmA , these three repeats are separated by nonhomologous intervening sequences highly enriched in serine , threonine , and asparagine . - pubmed
The mean proportion of LHRH neurons containing immunoreactive Fos was higher in the brains of young compared to middle - aged females in association with both the preovulatory ( p & lt ; 0.01 ) and the steroid - induced LH surge ( p & lt ; 0.001 ) . - pubmed
The time course of activation of LHRH neurons was delayed in the brains of aging females , and the proportion of double - labeled LHRH neurons remained elevated longer in the brains of young compared to middle - aged steroid - treated females . - pubmed
The proportion of nitrogen in whey protein : casein decreased from 85:15 to 54:46 during the 28 day time period . - pubmed
We have demonstrated that during anoxia intracellular K + decreases significantly in nerve cells and that this K + efflux is mediated by K + channels that are sensitive to ATP . - pubmed
P2 purinoceptors modulating noradrenaline release from sympathetic neurons in culture . - pubmed
ATP ( 1 mM ) inhibited , whereas 2 - methylthio - ATP ( 30 microM ) , a P2Y - selective purinoceptor agonist , increased electrically evoked release of [ 3H]noradrenaline from chick sympathetic neurons . - pubmed
The P2X - selective purinoceptor agonist alpha , beta - methylene - ATP ( 30 microM ) had no effect . - pubmed
The ATP - induced inhibition of release as well as the facilitation caused by 2 - methylthio - ATP was not affected by the selective adenosine ( P1 ) receptor antagonist 8 -( p - sulfophenyl )- theophylline ( 8 - PST ; 100 microM ) , but completely prevented by the non - selective P2 antagonist suramin ( 300 microM ) . - pubmed
MR imaging of PRL - secreting macro - adenoma has the advantage of being able to demonstrate the relationship of the tumor to the optic nerve and the cavernous sinus . - pubmed
Reactivation of NADH dehydrogenase ( complex I ) inhibited by 1 - methyl-4 -( 4 '- alkylphenyl ) pyridinium analogues : a clue to the nature of the inhibition site . - pubmed
Expression of the neurotoxicity of 1 - methyl-4 - phenyl-1,2,3,6 - tetrahydropyridine , following oxidation to 1 - methyl-4 - phenylpyridinium ion ( MPP + ) , is believed to involve inhibition of mitochondrial electron transport from NADH dehydrogenase ( complex I ) to ubiquinone . - pubmed
The low content of ASG in atrial cells of the bats is probably an indication of low demand for the natriuretic hormone of the granule , because , in such animals , and particularly in flight , conservation of fluid and electrolytes is of paramount importance . - pubmed
As in the growth cone , such changes involve Ca ( 2 +)-dependent pre- and postsynaptic processes , among which is the activation of protein kinase C. - pubmed
The membrane ectoenzyme 5 '- nucleotidase converts 5 '- AMP into adenosine which , in the nervous tissue , plays an important role as intercellular messenger . - pubmed
In the present report we have analyzed the distribution of 5 '- nucleotidase activity with electron microscopy , using a cerium - based method , at different postnatal histogenetic stages ( postnatal days ( PND ) 11 , 17 , 28 ) . - pubmed
Cytochemical patterns prove that the cerium - based method is suitable for demonstration of 5 '- nucleotidase - specific activity . - pubmed
We conducted a double - blind placebo - controlled cross - over trial to study the effect of the opiate antagonist , naloxone , on psychometric function together with plasma adrenocorticotrophic hormone ( ACTH ) , cortisol and prolactin . - pubmed
Cortisol and ACTH showed a sharp and significant rise after naloxone treatment in both RA and normal subjects ( P & lt ; 0.001 and P & lt ; 0.01 ) , but no difference was observed between the two groups . - pubmed
Although both IFN - gamma and penicillin induced aberrant chlamydial morphology and growth , we did not find evidence that these treatments elicited a classical stress response . - pubmed
In addition , T - cell - mediated lysis of Chlamydia - infected target cells treated with IFN - gamma or penicillin or grown in tryptophan - deficient media was examined . - pubmed
The increase of IFN gamma , the most active principle inducing neopterin release , was below the concentration needed for neopterin stimulation . - pubmed
As part of our efforts to understand the regulation of intracellular cAMP and to generate new targets for pharmacological intervention , we have cloned and characterized the first isozyme in a new family of cyclic nucleotide phosphodiesterases , PDE8A. - pubmed
PDE8A is most similar to PDE4 ( 38.5 % amino acid identity in the catalytic domain ) , but is clearly not a member of any of the seven known PDE families . - pubmed
The C - terminal 545 amino acids of PDE8A ( the region shared among all splice variants ) were expressed in baculovirus . - pubmed
The cAMP hydrolytic activity of PDE8A is not modulated by cGMP at concentrations up to 100 microM. - pubmed
PDE8A is insensitive ( up to 100 microM ) to a variety of PDE inhibitors including rolipram , zaprinast , vinpocetine , SKF-94120 , and IBMX , but is inhibited ( IC50 = 9 microM ) by the PDE inhibitor dipyridimole . - pubmed
To give PDE8A a descriptive name that distinguishes it from the other two known high affinity cAMP - specific phosphodiesterases ( PDE4 and PDE7 ) , we denote PDE8A as the high affinity cAMP - specific and IBMX - insensitive PDE . - pubmed
Regulation of endothelial cell myosin light chain phosphorylation and permeability by vanadate . - pubmed
Vanadate significantly increased EC MLC phosphorylation in a time - dependent manner ( maximal increase observed at 10 min ) and augmented both the MLC phosphorylation and permeability responses produced by thrombin , an agonist which rapidly increases tyrosine kinase activities . - pubmed
The vanadate - mediated increase in MLC phosphorylation was not associated with alterations in either phosphorylase A Ser / Thr phosphatase activities or in cytosolic [ Ca2 + ] but was strongly associated with significant increases in EC MLCK phosphotyrosine content . - pubmed
These data suggest that tyrosine phosphatase activities may participate in EC contractile and barrier responses via the regulation of the tyrosine phosphorylation status of EC MLCK . - pubmed
The resolution limit for these crystals is 3.9 A. Crystals of homoserine dehydrogenase grown in the presence of the co - factor NAD + have the tetragonal space group P41212 or its enantiomorph P43212 . - pubmed
Protein crystals grown in the presence of the product L - homoserine and the inert NAD+ analogue 3 - aminopyridine adenine dinucleotide belong to the monoclinic space group P21 with unit - cell parameters a = 58.8 , b = 104.2 , c = 120.7 A , beta = 91.9 degrees . - pubmed
The goal of this study was to test the hypothesis that the synthesis / release of hydroxyl radical accounts for impaired nitric oxide synthase - dependent dilatation of the basilar artery during diabetes mellitus . - pubmed
These findings suggest that impaired nitric oxide synthase - dependent dilatation of the basilar artery during diabetes mellitus may be related to the synthesis / release of hydroxyl radical . - pubmed
These results show that the antithrombotic activity of naroparcil is dependent on modification in the plasma GAG profile which inactivates thrombin via the HCII . - pubmed
Functional complementation cloning allowed us to identify the corresponding gene ( SAM3 ) , which encodes an integral membrane protein comprising 12 putative membrane spanning regions and belonging to the amino acid permease family . - pubmed
Among amino acid permease members , the closest relative of Sam3p is encoded by the YLL061w open reading frame . - pubmed
Disruption of YLL061w was shown to specifically lead to cells unable to use SMM as a sulfur source . - pubmed
Expression of hCB1 cannabinoid receptors abolished the Ca ( 2 + ) current inhibition by endogenous pertussis toxin - sensitive G ( i / o )- coupled receptors for norepinephrine ( NE ) and somatostatin ( SOM ) but not by endogenous pertussis toxin - insensitive G ( s )- coupled receptors for vasoactive intestinal polypeptide . - pubmed
Some hCB1 receptors were constitutively active because the cannabinoid receptor inverse agonist SR 141617A enhanced the Ca ( 2 + ) current . - pubmed
Some hCB1 receptors also appear to be precoupled to G ( i / o )- proteins because the cannabinoid agonist WIN 55,212 - 2 decreased the Ca ( 2 + ) current at a time when no G - proteins were available to couple to alpha ( 2 )- adrenergic and somatostatin receptors . - pubmed
In SCG neurons microinjected with a lower concentration of hCB1 cDNA , the effect of SR 141716A was reduced , and the response to NE and SOM was partially restored . - pubmed
Troglitazone , a new oral antidiabetic drug , is reported to be mostly metabolized to its conjugates and not to be oxidized by cytochrome P-450 ( P-450 ) enzymes . - pubmed
Hydrophilic agents such as carbon and aluminum oxide stimulate ingrowth of fibroblasts that secrete collagen . - pubmed
To prevent , at least partly , priming for dyskinesias can be attempted by delaying the need for levodopa , and/or reducing the cumulative dose of levodopa , using , for example , dopamine D2 agonists at the early stages of treatment . - pubmed
This basic motif ( Lys ( 40 -) Lys - Leu - Lys - Lys(44 ) ) , conserved among all the pathway - specific Smad proteins , is required for Smad 3 nuclear import in response to ligand . - pubmed
The effect of pH on the kinetics of the bioelectrocatalytic reduction of H ( 2 ) O ( 2 ) catalysed by horseradish peroxidase ( HRP ) has been studied at -50 mV vs. Agmid R : AgCl on HRP - modified Au electrodes placed in a wall - jet flow - through electrochemical cell . - pubmed
A favourable adsorption of C(His)rHRP on pre - oxidized Au from a protein solution at pH 6.0 provided a high and stable current response to H ( 2 ) O ( 2 ) due to its bioelectrocatalytic reduction based on direct ( mediator - less ) electron transfer ( ET ) between Au and the active site of HRP . - pubmed
The heterogeneous ET rate constant , k(s ) , calculated from experimental data on direct ET , on mediated ET in the presence of catechol as well as from microbalance data , increased more than 30 times when changing from nHRP to C(His)rHRP . - pubmed
For both forms of HRP , the increasing efficiency of bioelectrocatalysis with increasing [ H ( 3 ) O (+ ) ] was observed . - pubmed
The values of the apparent k(s ) between C(His)rHRP and Au changed from a value of 12+/-2 s(-1 ) in PBS at pH 8.0 to a value of 434+/-62 s(-1 ) at pH 6.0 ; a similar k(s)-pH dependence was also observed for nHRP , providing the possibility to consider the reaction mechanism involving the participation of a proton in the rate - determining step of the charge transfer . - pubmed
The apparent mass resolution of oligonucleotides in time - of - flight ( TOF ) mass spectrometers has been examined . - pubmed
Fasting lipid profiles , glucose and insulin were also measured . - pubmed
In GH - deficient subjects , there were inverse correlations between LDL cholesterol and FMD ( r = -0.40 , P & lt ; 0.05 ) and between FMD and the augmentation index ( r = - 0.58 , P & lt ; 0.01 ) . - pubmed
LDL cholesterol also decreased ( 3.5 + /- 0.8 vs. 4.2 + /- 0.8 mmol / l , P & lt ; 0.01 ) . - pubmed
Reaction suspensions were separated by centrifugation , and absorption measurements made for separated bilirubin , Fe , and protein supernatants . - pubmed
Concentrations of bilirubin , Ferrum and protein in supernatants decreased with increased NaOCl concentration . - pubmed
NaOCl had an effect on the protein component in blood . - pubmed
CsA concentrations were measured using a validated LC / MS - MS assay ( quantitation limit = 0.1 ng / mL ) . - pubmed
Reversed - phase liquid chromatography method to determine COL-3 , a matrix metalloproteinase inhibitor , in biological samples . - pubmed
In acini exposed to replication - defective or UV - inactivated Ad , carbachol - stimulated release of bulk protein and beta - hexosaminidase were significantly ( P< ; or = 0.05 ) inhibited to an extent proportional to the loss of rab3D - enriched mature secretory vesicles associated with these treatments . - pubmed
Activation of deoxycytidine kinase in lymphocytes is calcium dependent and involves a conformational change detectable by native immunostaining . - pubmed
Deoxycytidine kinase ( dCK ) , the principal deoxynucleoside salvage enzyme , plays a seminal role in the bioactivation of a wide array of cytotoxic nucleoside analogues . - pubmed
BAPTA - AM , a cell - permeable calcium chelator selectively inhibited the activation of dCK in a time- and concentration - dependent manner while extracellular calcium depletion had no effect . - pubmed
Denaturing Western blots of extracts from lymphocytes incubated with 2 - chlorodeoxyadenosine , aphidicolin and/or BAPTA - AM clearly demonstrated that dCK protein levels were unchanged during these treatments . - pubmed
Extracts from CdA - treated cells were much better recognized by the antibody raised against the C - terminal peptide of dCK than the BAPTA - AM - treated samples . - pubmed
These results indicate that the calcium - dependent activation of dCK is accompanied by a conformational change that renders the C - terminal epitope more accessible to the antibody . - pubmed
The mean population values of CL and V1 of gentamicin dosed once daily are in agreement with those described by others . - pubmed
Using a selective inducible nitric oxide synthase ( iNOS ) inhibitor , N ( 6 )-( iminoethyl )- lysine ( L - NIL ) , we explored the role of iNOS in the early pro - inflammatory signaling and acute lung injury in experimental abdominal aortic aneurysm rupture . - pubmed
Pulmonary permeability to ( 125 ) I - labeled albumin , myeloperoxidase ( MPO ) activity , cytokine levels , and semi - quantitative RT - PCR for mRNA were indicators of microvascular permeability , leuco - sequestration , and pro - inflammatory signaling , respectively . - pubmed
Lung tissue MPO activity was significantly increased in s + c compared to sham ( 2.80 + /- 0.32 versus 1.03 + /- 0.29 , P & lt ; 0.002 ) , and attenuated by L - NIL treatment ( 1.50 + /- 0.20 , P & lt ; 0.007 ) . - pubmed
Lung tissue iNOS activity was significantly increased in s + c compared to sham animals ( P & lt ; 0.05 ) , and attenuated by L - NIL treatment ( P & lt ; 0.05 ) . - pubmed
Therefore , trans - splicing of the SL1 leader sequence may serve at least two functions in nematodes , generation of an optimal 5'-untranslated region length and a specific sequence context ( SL1 ) for optimal translation of trimethylguanosine capped transcripts . - pubmed
In all of the culture systems , inclusion of ascorbic acid stimulated PRG4 secretion , and the source of PRG4 was immunolocalized to superficial cells . - pubmed
The marked up - regulation of PRG4 synthesis by ascorbic acid may have implications for cartilage homeostasis and prevention of osteoarthritic disease . - pubmed
The skeletal muscle calcium channel alpha - subunit gene CACNA1S is a major disease - causing gene for HypoPP , however , only three specific HypoPP - causing mutations , Arg528His , Arg1,239His and Arg1,239Gly , have been identified in CACNA1S to date . - pubmed
Normal prostate tissue is regulated by androgens , which activate the androgen receptor , a nuclear receptor transcription factor . - pubmed
Most prostate tumors retain androgen dependence , therefore , current therapies for advanced prostate cancer either reduce androgen levels or prevent binding to the androgen receptor . - pubmed
Although these relapsed tumors are androgen independent , they are still dependent on the androgen receptor for their growth and survival . - pubmed
METHODS : Cell proliferation was detected by sulforhodamine B ( SRB ) assay after treated with HY-1 at indicated doses . - pubmed
Cell cycle analysis was performed by flow cytometry , mitochondria membrane voltage change was assessed by rhodamine 123 staining , annexin V - PI apoptosis detecting kit and DNA agarose gel electrophoresis were used to identify apoptosis - inducing effect of HY-1 . - pubmed
It was recently reported that inhaled NO causes peripheral vasodilatation after treatment with a NO synthase ( NOS ) inhibitor . - pubmed
These findings suggested the possibility that inhibition of endogenous NOS uncovered the systemic vasodilating effect of NO or NO adducts absorbed via the lungs during NO inhalation . - pubmed
The alpha2A - adrenoceptor antagonist 2 -(( 4,5 - dihydro-1H - imidazol-2 - yl ) methyl )- 2,3 - dihydro-1 - methyl-1H - isoindole ( BRL 44408 ) ( 1 mg kg(-1 ) ) prolonged the hypothermic response to MDMA . - pubmed
Blood pressure actions of the three amphetamine derivatives may be at least partly due to alpha1 - adrenoceptor agonism or antagonism . - pubmed
The reversal of the hypothermic actions are at least partly due to alpha2A - adrenoceptor agonism since the hypothermic response was more prolonged with MDEA which exhibits low alpha2A - adrenoceptor potency , and effects of MDMA after alpha2A - adrenoceptor antagonism were similar to those of MDEA . - pubmed
Glutaredoxins ( GRXs ) can be subdivided into two subfamilies : dithiol GRXs with the CPY / FC active site motif , and monothiol GRXs with the CGFS motif . - pubmed
cerevisiae cells have two monothiol GRXs with the Trx - Grx structure ( ScGrx3 and ScGrx4 ) that locate at the nucleus and probably regulate the activity of transcription factors such as Aft1 , and one monothiol GRX with the Grx structure ( ScGrx5 ) that localizes at the mitochondrial matrix , where it participates in the synthesis of iron - sulphur clusters . - pubmed
PTP1B deficiency in immortalized neonatal hepatocytes prolonged insulin - induced tyrosine phosphorylation of the insulin receptor ( IR ) and IR substrates ( IRS ) - 1 , - 2 compared with wild - type control cells . - pubmed
The ligand was shown to be 17beta - estradiol and the binding affinities of EBP were demonstrated to be similar to those of rat estrogen receptors . - pubmed
Y - position cysteine substitution in type I collagen ( alpha1 ( I ) R888C / p. R1066C ) is associated with osteogenesis imperfecta / Ehlers - Danlos syndrome phenotype . - pubmed
The most common mutations in type I collagen causing types II - IV osteogenesis imperfecta ( OI ) result in substitution for glycine in a Gly - Xaa - Yaa triplet by another amino acid . - pubmed
Polyacrylamide gel electrophoresis ( PAGE ) of [ ( 3 ) H ]- proline labeled steady - state collagen reveals slight overmodification of the alpha1(I ) monomer band , much less than expected for a substitution of a neighboring glycine residue , and a faint alpha1(I ) dimer . - pubmed
This report on Y - position substitutions in type I collagen extends the range of phenotypes caused by nonglycine substitutions and shows that , similar to X- and Y - position substitutions in types II and III collagen , the phenotypes resulting from nonglycine substitutions in type I collagen are distinct from those caused by glycine substitutions . - pubmed
Calprotectin is a novel 36 kDa calcium - binding protein of the S100 family and appears to be a nonspecific marker of inflammation . - pubmed
Estrogens and antiestrogens activate hPXR . - pubmed
A significant proportion ( 54 % ) of compounds with estrogenic activity or able to bind ER were found to be hPXR activators : in particular , antiestrogens , mycoestrogens and phthalates . - pubmed
PAD patients often show elevated triglycerides and reduced HDL - cholesterol , two lipid abnormalities usually accompanied by decreased LDL size in the " atherogenic lipoprotein phenotype " ( ALP ) . - pubmed
Patients had higher prevalence of hypertension ( p=.0132 ) , smoking ( p<.0020 ) and diabetes ( p=.0024 ) , with lower HDL - cholesterol ( p<.0001 ) and increased triglycerides ( p=.0057 ) ; LDL size was smaller ( p<.0001 ) , with decreased larger subclasses ( LDL - I , p<.0001 ; LDL - IIA , p=.0068 ) and increased smaller particles ( LDL - IIIA , p<.0001 ; LDL - IIIB , p=.0013 ; LDL - IVA , p=.0029 ; LDL - IVB , p<.0001 ) . - pubmed
The concomitant presence of high triglycerides , low HDL - cholesterol and elevated small , dense LDL in patients was 26 % ( versus 0 % controls , p=.0024 ) . - pubmed
2 '- Deoxycytidylate deaminase [ or deoxycytidine-5 '- monophosphate ( dCMP ) deaminase , dCD ] catalyzes the deamination of dCMP to deoxyuridine-5 '- monophosphate to provide the main nucleotide substrate for thymidylate synthase , which is important in DNA synthesis . - pubmed
Their SOD activities were similar and 2- to 3 - fold more potent than the standard compound EUK-134 . - pubmed
Their catalase - like activity was lower than that of EUK-134 in the initial conversion rate ; however , some analogs exhibited a better catalytic turnover number . - pubmed
This increase in MAP was associated with a significant increase in plasma angiotensin - converting enzyme activity and angiotensin II levels . - pubmed
Effect of ZO pretreatment on radiation - induced changes in glutathione ( GSH ) , glutathione - S - transferase ( GST ) , superoxide dismutase ( SOD ) , catalase ( CAT ) and lipid peroxidation ( LPx ) levels was also analyzed . - pubmed
A significant ( p & lt ; 0.001 ) reduction in micronucleated polychromatic , normochromatic erythrocytes , increased PCE / NCE ratio , increase in the GSH , GST , SOD , CAT and decreased LPx levels were observed in ZO pretreated group when compared to the irradiated animals . - pubmed
It was not or only partially inhibited by several modulators of the cell surface NTPDases , but was strongly inhibited upon oxidative cross - linking by copper phenanthroline . - pubmed
Moreover the TS samples showed more antioxidant activity in some aspects and also exhibit more flavonoid content . - pubmed
However , in the second experiment , nine male and twelve female participants subjected to a cold pressor test ( CPT ) before and after Red Bull consumption showed a significant increase in blood sugar levels pre- and post Red Bull consumption . - pubmed
In addition , nanoencapsulation permits the cells to respond to changes in extracellular glucose and other insulin secretagogues by releasing insulin with a profile similar to that of nonencapsulated cells . - pubmed
This review examines the new evidence supporting the role of nontriglyceride fatty acid metabolites in causing NASH and how adipose and muscle insulin resistance contribute to hepatic lipotoxicity . - pubmed
Injection of an anti - PI ( 4,5 ) P ( 2 ) antibody inhibited invadopodia formation along with gelatin degradation activity . - pubmed
Sequestering of PI ( 4,5 ) P ( 2 ) by overexpression of the phospholipase C ( PLC ) delta1 - pleckstrin homology ( PH ) domain , a specific probe for PI ( 4,5 ) P ( 2 ) , also blocked invadopodia formation , while a mutated PLCdelta1 - PH domain that lacks PI(4,5)P(2)-binding activity had no effect . - pubmed
Cellular PI ( 4,5 ) P ( 2 ) production is mainly mediated by type - I phosphatidylinositol 4 - phosphate 5 - kinase ( PIP5KI ) family proteins , which include PIP5KIalpha , Ibeta , and Igamma . - pubmed
We screened a library of 37 indoles for those that selectively change fluorescence in the presence of prefibrillar amyloid - beta ( Abeta ) . - pubmed
Using this probe in combination with electron microscopy , we found that prefibrils are largely depleted during Abeta aggregation in vitro but that they remain present after the apparent saturation of the ThT signal . - pubmed
These results suggest that a combination of TROL and ThT provides greater insight into the process of amyloid formation by Abeta . - pubmed
In addition , we found that TROL also recognizes other amyloid - prone proteins , including ataxin-3 , amylin , and CsgA. - pubmed
The brain cannabinoid ( CB ( 1 ) ) receptor is unique in that it lacks the interloop E2 disulfide linkage to the transmembrane ( TM ) helical bundle , a characteristic of many GPCRs . - pubmed
Recent mutation studies of the CB ( 1 ) receptor , however , suggest the presence of an alternative intraloop disulfide bond between two E2 Cys residues . - pubmed
Considering the oxidation state of these Cys residues , we determine the molecular structures of the 17 - residue E2 in the dithiol form ( E2 ( dithiol ) ) and in the disulfide form ( E2(disulfide ) ) of the CB ( 1 ) receptor in a fully hydrated 1 - palmitoyl-2 - oleoyl - sn - glycero-3 - phosphocholine bilayer , using a combination of simulated annealing and molecular dynamics simulation approaches . - pubmed
We characterize the CB ( 1 ) receptor models with these two E2 forms , CB ( 1 )( E2 ( dithiol ) ) and CB ( 1 )( E2 ( disulfide ) ) , by analyzing interaction energy , contact number , core crevice , and cross correlation . - pubmed
Dopamine ( DA ) produced by TIDA neurons directly inhibits prolactin secretion and , therefore , to stimulate prolactin secretion , estrogens inhibit TIDA neurons to decrease DA production . - pubmed
Chronic E(2 ) exposure significantly increased the expression of glial fibrillary acidic protein and the concentrations of interleukin-1beta ( IL-1beta ) and nitrate in the AN that contains perikarya of TIDA neurons and increased nitration of TH in the median eminence ( ME ) that contains the terminals . - pubmed
PYR-41 is a small molecule with previously described UBE1 inhibitory activity . - pubmed
Detailed evaluation of PYR-41 activity demonstrated that PYR-41 inhibited UBE1 activity but also had equal or greater inhibitory activity against several deubiquitinases ( DUBs ) in intact cells and purified USP5 in vitro . - pubmed
Both UBE1 and DUB inhibition were mediated through PYR-41 - induced covalent protein cross - linking which paralleled the inhibition of the target proteins enzymatic activity . - pubmed
PYR-41 also mediated cross - linking of specific protein kinases ( Bcr - Abl , Jak2 ) to inhibit their signaling activity . - pubmed
Blood samples were collected up to 58 hours post - first dose for plasma concentrations of modafinil and its metabolites . - pubmed
Cy5.5 - Gly - Pro - Leu - Gly - Val - Arg - Gly -( TDOPA ) 3 - flower - like gold - Fe3O4 optical nanoparticles Extracellular matrix ( ECM ) adhesion molecules consist of a complex network of fibronectins , collagens , chondroitins , laminins , glycoproteins , heparin sulfate , tenascins , and proteoglycans that surround connective tissue cells , and they are mainly secreted by fibroblasts , chondroblasts , and osteoblasts ( 1 ) . - pubmed
PEG - AuNPs have been being studied as cancer CT imaging and photothermal agents ( 7 ) . - pubmed
The peptide Gly - Pro - Leu - Gly - Val - Arg - Gly - Cys - NH2 was found to be a MMP substrate and is cleaved between the Leu and Gly residues . - pubmed
NIR fluorescence signal will increase when the Leu - Gly bond is cleaved by MMPs , releasing Cy5.5 - containing fragments . - pubmed
Cy5.5 - MMP - AuNPs are being developed for NIR fluorescence imaging of MMPs expressed in tumors , atherosclerosis , myocardial infarction , and other diseases . - pubmed
( 14 ) replaced the AuNP with Au - Fe3O4 , a composite NP shaped like a flower , to induce a fluorescently quenched state to the overall nanostructure . - pubmed
The MMP peptide was covalently linked to an anchoring unit , Lys - tridihydrophenylalanine ( Lys - TDOPA ) on the surface of the iron oxide NP . - pubmed
Cy5.5 - Gly - Pro - Leu - Gly - Val - Arg - Gly -( Lys - TDOPA)3 - flower - like gold - Fe3O4 optical NPs ( FANPs ) have been evaluated for imaging protease expression in vivo . - pubmed
VEGF - D promotes tumor metastasis by regulating prostaglandins produced by the collecting lymphatic endothelium . - pubmed
We identified regulation of PGDH , the key enzyme in prostaglandin catabolism , in endothelial cells of collecting lymphatics , as a key molecular change during VEGF - D - driven tumor spread . - pubmed
The VEGF - D - dependent regulation of the prostaglandin pathway was supported by the finding that collecting lymphatic vessel dilation and subsequent metastasis were affected by nonsteroidal anti - inflammatory drugs ( NSAIDs ) , known inhibitors of prostaglandin synthesis . - pubmed
Our data suggest a control point for cancer metastasis within the collecting lymphatic endothelium , which links VEGF - D / VEGFR-2 / VEGFR-3 and the prostaglandin pathways . - pubmed
Over the past 5 y , research has revealed that the basic leucine zipper superfamily of transcription factors plays the central role in the AAR . - pubmed
Measured FTIR , CP / MAS , and high - resolution ( 13 ) C NMR spectra indicate that H - bond in the PyO HCl complex in polar solvent can potentially be more ionic than in the crystal . - pubmed
RESULTS : At 7 d after the first SAH , protein levels of phospho - Akt and ERalpha were significantly decreased and caspase-3 was significantly increased in the dentate gyrus . - pubmed
Those actions were reversed by E2 and blocked by ICI 182,780 and methyl - piperidino - pyrazole , but not R , R - tetrahydrochrysene . - pubmed
However , there were no significant changes in the expression of the protein levels of phospho - Akt , ERalpha , ERbeta , and caspase-3 , and DNA fragmentation after SAH . - pubmed
CONCLUSIONS : The present study shows that a beneficial effect of E2 in attenuating SAH - induced apoptosis is associated with activation of the expression of phospho - Akt and ERalpha , and alteration in caspase-3 protein expression via an ERalpha - dependent mechanism in the dentate gyrus . - pubmed
Melatonin selectively activated Gq and stimulated phosphoinositide ( PI ) hydrolysis in freshly dispersed and cultured muscle cells . - pubmed
Melatonin - induced PI hydrolysis and contraction were blocked by a non - selective MT1 / MT2 antagonist luzindole ( 1 muM ) , but not by a selective MT2 antagonist 4P - PDOT ( 100 nM ) , and by the PLC inhibitor U73122 . - pubmed
MT2 selective agonist IIK7 ( 100 nM ) had no effect on PI hydrolysis and contraction . - pubmed
Activation of these receptors causes stimulation of PI hydrolysis and increase in cytosolic Ca ( 2 + ) , and elicits muscle contraction . - pubmed
Product analyses were performed with the application of ESI - MS and ( 1 ) H - NMR techniques . - pubmed
In cellular systems , two inhibitors , KH7 and catechol estrogens , proved specific for sAC , and 2 ' , 5 '- dideoxyadenosine proved specific for tmACs . - pubmed
Insulin sensitivity and beta - cell function were evaluated by oral glucose tolerance testing . - pubmed
Here we report a MOF that consists of glycyl - serine dipeptides coordinated to metal centres , and has a structure that evolves from a solvated porous state to a desolvated non - porous state as a result of ordered cooperative , displacive and conformational changes of the peptide . - pubmed
First - line triplet chemotherapy plus bevacizumab ( FIr - B / FOx ) can improve efficacy of metastatic colorectal cancer ( MCRC ) , KRAS wild - type and mutant . - pubmed
The design and synthesis of novel SGLT2 inhibitors : C - glycosides with benzyltriazolopyridinone and phenylhydantoin as the aglycone moieties . - pubmed
This report describes the design , synthesis and structure - activity relationship ( SAR ) of C - glycosides with benzyltriazolopyridinone and phenylhydantoin as the aglycone moieties as novel SGLT2 inhibitors . - pubmed
Is preference for ovulatory female 's faces associated with men 's testosterone levels ? - pubmed
Testosterone was not predictive for the chosen face ; regardless of testosterone level men preferred the ovulatory woman . - pubmed
There have been many new developments for treatment of advanced NETs including somatostatin analogs , hepatic artery embolization , chemotherapy , interferons , mammalian target of rapamycin inhibitors and radiolabeled somatostatin analogs . - pubmed
While the ' C5100U5101 ' natural mutations in the upstream ORF1 coding region replace ' alanine ' for the conserved ' valine ' , the ' G5117G5118 ' doublet resides in the downstream non - coding / promoter region of ORF3 gene . - pubmed
dianthus to 350 m depth ( pHT 8.02 ; pCO2 448 muatm , Omegaara 2.58 ) and to a 3 m depth CO2 seep in oligotrophic waters ( pHT 7.35 ; pCO2 2879 muatm , Omegaara 0.76 ) and found that the transplants calcified at the same rates regardless of the pCO2 confirming their resilience to acidification , but at significantly lower rates than corals that were fed in aquaria . - pubmed
Further , using cell cycle analysis , MTT , luciferase and knockdown experiments , we found evidence to suggest that ETS1 is central in the development of the proliferative , metastatic and invasive phenotype of ccRCC cells . - pubmed
Purified polysaccharide SSP II - a was successfully obtained using diethylaminoethanol - Sepharose and Sepharose CL-6B column chromatography . - pubmed
SSP II - a was found to be an acidic polysaccharide fraction with an average molecular weight of 168kDa and composed of rhamnose , glucuronic acid , galacturonic acid , glucose , galactose and arabinose . - pubmed
In vitro antioxidant activity assays suggested that SSP II - a presents high scavenging activity toward superoxide anion , hydroxyl , and 2,2 '- azino - bis ( 3 - ethylbenz - thiazoline-6 - sulfonic acid ) radicals but relatively lower scavenging activity toward 1,1 - diphenyl-2 - picrylhydrazyl radicals . - pubmed
A Case of Squamous Cell Carcinoma Harboring an EML4 - ALK Rearrangement that Was Unsuccessfully Treated with the ALK Inhibitor Alectinib . - pubmed
BACKGROUND : To assess differences between four different voxel - based dosimetry methods ( VBDM ) for tumor , liver , and lung absorbed doses following ( 90 ) Y microsphere selective internal radiation therapy ( SIRT ) based on ( 90 ) Y bremsstrahlung SPECT / CT , a secondary objective was to estimate the sensitivity of liver and lung absorbed doses due to differences in organ segmentation near the liver - lung interface . - pubmed
Special attention is given to the involvement of pigment epithelial cells , macrophages , hyalocytes , gliocytes , supporting fibers ( Muller 's cells ) , mediators of inflammation , such as cytokines , chemokines , and growth factors , with reference to their interaction in the processes of proliferation and development of retinal membranes . - pubmed
This study compared the efficacy and safety of fixed dose combination ( FDC ) of arterolane maleate and piperaquine phosphate ( PQP ) with chloroquine in the treatment of uncomplicated vivax malaria . - pubmed
The median parasite clearance time was 24 h in FDC of arterolane maleate and PQP group and 26 h in chloroquine group ( Log - rank , p = 0.2264 ) . - pubmed
In PP population , day 28 cure rates were 100 % in both the groups ( 95 % CI ( 96.52 , 100.0 for FDC of arterolane maleate and PQP and 96.73 , 100.0 in chloroquine group ) ) . - pubmed
Incidence of adverse events was 82.4 % in the FDC of arterolane maleate and PQP group and 85.4 % in the chloroquine group . - pubmed
The commonly reported clinical adverse events in the FDC of arterolane maleate and PQP versus chloroquine group were vomiting ( 5.0 vs 5.1 % ) , headache ( 1.3 vs 3.2 % ) and prolonged QT ( 1.9 vs 3.2 % ) . - pubmed
The pharmacokinetic analysis indicates that arterolane maleate is well absorbed and has a relatively short t1/2 of 3.2 h. Piperaquine is also well absorbed after oral administration with a t1/2 of about 228.33 h. CONCLUSIONS : The study showed that FDC of arterolane maleate and PQP effectively cured vivax malaria and attained acceptable level of cure up to day 28 . - pubmed
The Cytoplasmic Carbonic Anhydrases betaCA2 and betaCA4 Are Required for Optimal Plant Growth at Low CO2 . - pubmed
We hypothesize that cytoplasmic betaCAs are required to modulate inorganic carbon forms needed in leaf cells for carbon - requiring reactions such as photosynthesis and amino acid biosynthesis . - pubmed
Comparing transfer DNA knockout lines with wild - type plants , there was no reduction in the growth rates of the single mutants , betaca2 and betaca4 However , the growth rate of the double mutant , betaca2betaca4 , was reduced significantly when grown at 200 muL L(-1 ) CO2 The reduction in growth of the double mutant was not linked to a reduction in photosynthetic rate . - pubmed
This suggests the cytoplasmic CAs play an important but not previously appreciated role in amino acid biosynthesis . - pubmed
BACKGROUND : Misuse and/or dependence upon non - prescription combination analgesics containing codeine ( NP - CACC ) can result in serious physiological and psychological harms . - pubmed
LC - MS / MS analysis of steroids in the clinical laboratory . - pubmed
Liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) is a powerful tool that is changing the way we analyse steroids in the clinical laboratory . - pubmed
The chromobox 7 ( CBX7 ) protein of the polycomb repressive complex 1 ( PRC1 ) functions to repress transcription of tumor suppressor p16 ( INK4a ) through long noncoding RNA , ANRIL ( antisense noncoding RNA in the INK4 locus ) directed chromodomain ( ChD ) binding to trimethylated lysine 27 of histone H3 ( H3K27me3 ) , resulting in chromatin compaction at the INK4a / ARF locus . - pubmed
Our Class A compounds , a series including analogues of the previously reported MS452 , inhibit CBX7ChD / methyl - lysine binding by occupying the H3K27me3 peptide binding site , whereas our Class B compound , the newly discovered MS351 , appears to inhibit H3K27me3 binding when CBX7ChD is bound to RNA . - pubmed
Our crystal structure of the CBX7ChD / MS351 complex reveals the molecular details of ligand recognition by the aromatic cage residues that typically engage in methyl - lysine binding . - pubmed
Thus , MS351 represents a new class of ChD antagonists that selectively targets the biologically active form of CBX7 of the PRC1 in long noncoding RNA- and H3K27me3 - directed gene transcriptional repression . - pubmed
PURPOSE : To investigate the effect of blocking renin - angiotensin system by direct renin inhibition using aliskiren on the renal dysfunction following reversible unilateral ureteral obstruction ( UO ) . - pubmed
Group - Alsk ( n=12 ) received aliskiren ( 30 mg / kg / day ) dissolved in water starting one day before creating UO and continued until the terminal experiment five days post reversal when renal functions were measured using clearance techniques . - pubmed
Aliskiren also decreased the gene expressions of NGAL , KIM-1 and p53 . - pubmed
CONCLUSION : Direct renin inhibition by aliskiren appears to have protective effect on the renal dysfunction and on the markers of renal injury following UO indicating a potential clinical benefit of this agent . - pubmed
The inhibitor ifenprodil , and related phenylethanoamine compounds specifically targeting GluN1 / GluN2B NMDARs have neuroprotective activity . - pubmed
We applied genetically encoded unnatural amino acids ( Uaas ) and monitored the functional changes in living cells with photo - cross - linkers specifically incorporated at the ifenprodil binding interface between GluN1 and GluN2B subunits . - pubmed
We report constraining the NTD domain movement , by a light induced crosslinking bond that introduces minimal perturbation to the ligand binding , specifically impedes the transduction of ifenprodil but not Zn2 + inhibition . - pubmed
A particularly versatile CBT is 6 - amino-2 - cyanobenzothiazole ( ACBT ) , which has an amine handle for straight - forward derivatisation . - pubmed
EPR - juice had 9- and 5 - fold higher contents of phenolic compounds compared with the NEB- and EB - juices , respectively . - pubmed
Effect of the low content and stability of phenolics was noticed as loss of the visual color in the NEB - juice during storage . - pubmed
The decrease in monomeric anthocyanins and the increase in phenolic acids were most severe in the NEB - juice , whereas the most significant decline in hydroxycinnamic acid derivatives occurred in the enzyme - treated juices . - pubmed
Formation of Hexagonal - Close Packed ( HCP ) Rhodium as a Size Effect . - pubmed
Previous studies on the structural and functional properties of rhodium are based on the face - centered - cubic ( fcc ) structure in the bulk form . - pubmed
The NO donor prodrug JS - K ( O2 -( 2.4 - dinitrophenyl ) 1 -[( 4 - ethoxycarbonyl ) piperazin-1 - yl ] diazen-1 - ium-1,2 - diolate ) allows cell - type specific intracellular NO release via enzymatic activation by glutathione - S - transferases overexpressed in glioblastoma multiforme . - pubmed
Increases in the intracellular Ca2 + concentration in pancreatic islets , resulting from the Ca2 + mobilization from the intracellular source through the ryanodine receptor , are essential for insulin secretion by glucose . - pubmed
Cyclic ADP - ribose , a potent Ca2 + mobilizing second messenger synthesized from NAD + by CD38 , regulates the opening of ryanodine receptor . - pubmed
This report outlines the construction of an advanced , exquisite photoswitchable enzyme cascade on the basis that tyrosinase ( TYR ) catalyzes the generation of dihydroxyphenylalanine ( DOPA ) coordinated TiO2 nanoparticles ( NPs ) to form a light responsive nano - trigger that subsequently photoactivates the enzymatic activity of horseradish peroxidase ( HRP ) . - pubmed
Prostate cancer stem - like cells ( PCSCs ) are not only enriched in the CD44+PSA-/lo subpopulation but also employ androgen - independent signaling mechanisms for survival . - pubmed
CD44 + PCSCs defy androgen deprivation , resist chemo- and radiotherapy and are highly tumorigenic . - pubmed
Among a subset of tyrosine kinases crucial for PCSC survival , was a non - receptor tyrosine kinase , ACK1 / TNK2 , a critical regulator of castration resistant prostate cancer ( CRPC ) growth . - pubmed
Consistently , activated ACK1 as measured by phosphorylation at Tyr284 was significant in the CD44+PSA-/lo population . - pubmed
During adaptive evolution on the first carbon source , 4 - Hydroxyphenylacetic acid ( HPA ) , recombining populations adapted better , which was likely mediated by acquiring the hpa operon from the donor . - pubmed
Typical alpha - proteobacterial methylotrophy genes were found : pyrroloquinoline quinone ( PQQ )- dependent methanol dehydrogenase ( MDH ) ( mxaF and xoxF1 - 4 ) , methylotrophy regulatory proteins ( mxbDM and mxcQE ) , PQQ synthesis , H4F pathway , H4MPT pathway , formate oxidation , serine cycle , and ethylmalonyl - CoA pathway . - pubmed
SDS - PAGE and subsequent LC - MS analysis , and qPCR analysis revealed that MxaF and XoxF1 were the dominant MDH in the absence or presence of lanthanum ( La3 + ) , respectively . - pubmed
The growth of MDH gene - deletion mutants on alcohols and qPCR results indicated that mxaF and xoxF1 are also involved in ethanol and propanol oxidation , xoxF2 participates in methanol oxidation in the presence of La3 + , while xoxF3 was associated with methanol and ethanol oxidation in the absence of La3 + , implying that XoxF3 is a calcium ( Ca2 +)-binding XoxF. - pubmed
Four Ln3 + such as La3 + , cerium ( Ce3 + ) , praseodymium ( Pr3 + ) , and neodymium ( Nd3 + ) served as cofactors for XoxF1 by supporting DeltamxaF growth on methanol . - pubmed
Here , we show that proteolytic destruction of the key regulator Mcl-1 during an extended mitosis requires the anaphase - promoting complex or cyclosome ( APC / C ) and is independent of another ubiquitin E3 ligase , SCFFbw7 Using live - cell imaging , we show that the loss of Mcl-1 during mitosis is dependent on a D box motif found in other APC / C substrates , while an isoleucine - arginine ( IR ) C - terminal tail regulates the manner in which Mcl-1 engages with the APC / C , converting Mcl-1 from a Cdc20 - dependent and checkpoint - controlled substrate to one that is degraded independently of checkpoint strength . - pubmed
Gender Differences in the Hepatotoxicity and Toxicokinetics of Emodin : The Potential Mechanisms Mediated by UGT2B7 and MRP2 . - pubmed
A genome - wide association study ( GWAS ) identified that rs11726899 located within ~50 kb of the transcript of UGT2B could significantly affect emodin metabolism . - pubmed
Therefore , gender differences in the hepatotoxicity and toxicokinetics of emodin are potentially mediated by the coupling of UGT2B7 and MRP2 in vivo . - pubmed
AIM : To study the influence of adenosine receptor ligands and hypoxic preconditioning on carbohydrate metabolism in global brain ischemia . - pubmed
RESULTS AND CONCLUSION : Adenosine , an agonist of A1 adenosine receptors , and hypoxic preconditioning exert a neuroprotective effect on brain cells . - pubmed
The blocker of A1 adenosine receptors aggravates metabolic disturbances in the brain and removes the protective effect of cyclopenyladenosine and ischemic preconditioning . - pubmed
Although , mortality of MSC - TV group decreased 14.7 % as compared to TAA group ( p = 0.200 ) , MSC + Scaffold group had the lowest mortality ( 31.4 % ) ( p = 0.013 ) . - pubmed
The expression of Notch2 and Sox2 during extensive - passage , low - oxygen culture and differentiation conditions were analyzed to confirm that the presence of these signature proteins directly correlates with the ability of NSCs to form new neurospheres and differentiate into multiple cell types . - pubmed
The glycosylphosphatidylinositol ( GPI ) anchored glycoprotein Thy-1 has been prevalently expressed on the surface of various cell types . - pubmed
The rapid recovery phase seen in the absence of calcium , was dependent on extracellular bicarbonate and could be inhibited by S0859 , a potent Na / HCO3 cotransporter inhibitor . - pubmed
Immunostaining and PCR data , showed that NBCe1 ( SLC4A4 ) and NBCn1 ( SLC4A7 ) were expressed in the cell population and that the pHi recovery in the radial glial - like cells after calcium removal was mediated mainly by the electrogenic sodium bicarbonate transporter NBCe1 ( SLC4A4 ) . - pubmed
Our results indicate that extracellular calcium might hamper pHi regulation and Na / HCO3 cotransporter activity in a brain injury microenvironment . - pubmed
In this study , we demonstrate that the partition of proteins in salt - containing ATPS ( SATPS ) depends only on the relation between the protein - surface amino acids and the type of salt using SATPS . - pubmed
Interestingly , the partition coefficients of the proteins correlated to those of the combination of the amino acids in the surface of the protein with the correlation coefficients of & gt;0.9 . - pubmed
It shows a splendid limit of detection of soluble arsenic down to a few ppb level , much below than the MCL reported value , without being interfered by any other ions . - pubmed
FDG PET / CT Findings of Unsuspected Pulmonary Epithelioid Angiosarcoma Manifested as a Brain Metastasis . - pubmed
FDG PET / CT showed increased uptake corresponding to lesions in the right frontal lobe of the brain , the left upper lobe of lung , and the left adrenal gland , respectively . - pubmed
To analyze the mechanisms implicated in autotolerance failure , we evaluated CD4+CD69 + and CD4+CD25+FoxP3 + T cells and interleukin profiles in a pristane - induced SLE experimental model . - pubmed
Thus , the goal of this study was to assess the relationships among serum estradiol ( E2 ) and endothelial growth factor ( VEGF ) levels , MDSC ratios , and pregnancy outcome associated with IVF . - pubmed
Forage legumes - grazing lambs outperformed SI lambs in LT nutritional quality , with more conjugated linoleic acids and n-3 PUFAs , especially 18:3n-3 , eicosapentaenoic and docosahexaenoic acids ( P & lt ; 0.001 ) , and thus lower n-6 PUFA / n-3 PUFA and 18:2 n-6/18:3 n-3 ratios ( P & lt ; 0.001 ) . - pubmed
The liquid chromatography - mass spectrometry ( LC - MS ) analysis of EAF demonstrated the presence of phenolic metabolites that belong to three structurally related groups as majority compounds : caffeoylquinic acid , coumaroyl - glucoside , and caffeoyl - glucoside / deoxyhexosyl - caffeoyl glucoside derivatives . - pubmed
The following low - pressure limiting rate coefficients were obtained for T = 120 - 400 K and He bath gas ( in cm6 molecule-2 s-1 , uncertainty + -sigma at 180 K ): log10(k , Ni+ + N2 ) = -27.5009 + 1.0667log10(T ) - 0.74741(log10(T))2 , sigma = 29 % ; log10(k , Ni+ + O2 ) = -27.8098 + 1.3065log10(T ) - 0.81136(log10(T))2 , sigma = 32 % ; log10(k , Ni+ + CO2 ) = -29.805 + 4.2282log10(T ) - 1.4303(log10(T))2 , sigma = 28 % ; log10(k , Ni+ + H2O ) = -24.318 + 0.20448log10(T ) - 0.66676(log10(T))2 , sigma = 28 % ) . - pubmed
Parthenolide Has Negative Effects on In Vitro Enhanced Osteogenic Phenotypes by Inflammatory Cytokine TNF - alpha via Inhibiting JNK Signaling . - pubmed
PTL had no significant effects on hPDC viability or osteoblastic activities , whereas TNF - alpha had positive effects on the in vitro osteoblastic differentiation of hPDCs . - pubmed
Treatment with 1 microM PTL did not affect TNF - alpha - treated hPDCs ; however , 5 and 10 microM PTL treatment decreased the histochemical detection and activity of alkaline phosphatase ( ALP ) , alizarin red - positive mineralization , and the expression of ALP and osteocalcin mRNA . - pubmed
JNK phosphorylation decreased significantly in TNF - alpha - treated hPDCs pretreated with PTL . - pubmed
These results suggested that PTL exerts negative effects on the increased osteoblastic differentiation of TNF - alpha - treated hPDCs by inhibiting JNK signaling . - pubmed
A facile scalable approach is presented for the rational design of multidimensional , multilayered sand - clock - like UCNPs ( denoted as UCCKs ) bounded with high index facets , with a tunable Nd3 + content , and without a template or multiple complicated reaction steps . - pubmed
Graphical abstract We developed novel multidimensional multilayered sand - clock - like upconversion nanostructures composed of a spherical inner core ( NaYF4 : Yb , Er , Ca ) , hexagonal intermediate shell ( NaYF4 : Yb , Ca ) and two up - down outer shell ( NaNdF4 : Yb , Ca ) with controllable Nd3 + as an efficient and safe probe for bioimaging applications without any quenching effect . - pubmed
Further molecular mechanism investigation showed that LOX inhibited the Fas / FasL and p53 pathways . - pubmed
We show that reducing glycolysis both lowers steady - state levels of ATP and stabilizes Smoothened ( Smo ) , the 7 - pass transmembrane protein that transduces the Hh signal . - pubmed
We show that glycolysis is required to maintain the plasma membrane potential and that plasma membrane depolarization blocks cellular uptake of N - acylethanolamides - lipoprotein - borne Hh pathway inhibitors required for Smo destabilization . - pubmed
Among the 67 cefoxitin resistant strains , 30(44.78 % ) AmpC beta - lactamase producers were detected by MTDT and 59(88.06 % ) were detected by PCR . - pubmed
The dominant genotype found was CIT ( 62.69 % ) followed by DHA ( 53.73 % ) . - pubmed
The anti - androgenic property depends on the androgen receptor ( AR ) nuclear translocation ( AR trafficking and its subnuclear distribution ) , the inhibition of 5alpha - reductase activity ( it possesses higher activity compared to other available progestins ) , and the increase on sexual hormone binding globulin ( SHBG ) levels if combined with an estrogenic counterpart . - pubmed
Polyguanylate- and poly ( 2 '- O - methyl ) uridylate - Sepharose have been prepared for affinity chromatography of DNA polymerases of viral origin ( reverse transcriptase ) . - pubmed
Both cellular DNA polymerases and reverse transcriptase bind to polyguanylate - Sepharose . - pubmed
The cellular polymerases can be eluted from the column between 0.32 and 0.42 M NaCl while reverse transcriptase eluted between 0.56 and 0.78 M NaCl . - pubmed
However , only reverse transcriptase adheres to poly ( 2 '- O - methyl ) uridylate - Sepharose and can be eluted at approximately 0.35 M NaCl . - pubmed
[ Epidemiological and clinical significance of long - term carrier state of the Australia antigen ( HB - Ag ) ] . - pubmed
Polypeptide segments connecting Cys 46 to Cys 63 in HiPIP and Cys 18 to Cys 35 in ferredoxin are analogous in the sense that they are the same length , they connect equivalent cysteinyl sulfur atoms , and they have similar , twisted antiparalled beta conformations . - pubmed
Tyrosine residues 19 ( HiPIP ) and 2 ( ferredoxin ) are analogous in the sense that they interact closely with equivalent inorganic sulfur atoms . - pubmed
Potent inhibition of chloramphenicol acetyl transferase ( CAT ) by Triton X-100 and Nonidet P-40 was observed . - pubmed
The CAT activity was also moderately inhibited by sodium deoxycholate and sodium dodecyl sulfate , and least by Tween 20 . - pubmed
A remarkable reduction in plasma levels of FSH ( P less than 0.001 ) , LH ( P less than 0.05 , P less than 0.01 ) , PRL ( P less than 0.05 , P less than 0.001 ) and T ( P less than 0.001 ) along with significant diminution in the activities of testicular delta 5 - 3 beta - HSD ( P less than 0.001 ) and 17 beta - HSD ( P less than 0.001 ) were observed following lithium treatment for 14 and 21 days . - pubmed
LCNA was decreased after lithium chloride treatment for 14 and 21 days ( P less than 0.001 ) . - pubmed
Purified fibrinogen Ledyard had a prolonged polymerization , which was somewhat corrected by addition of Ca2 + ions . - pubmed
Steady - state kinetic parameters were evaluated for release of FPA by thrombin . - pubmed
When the concentration of fibrinogen Ledyard was corrected to 50 % of total protein , because only 50 % of fibrinogen Ledyard can release FPA , the kinetic constants were similar to those of control fibrinogen ( Km = 7.5 mumol / L for A alpha chain , kcat = 54 s-1 ) . - pubmed
The sequence of the amino terminus of A alpha chain showed that Arg in position 16 was replaced by Cys in the abnormal molecules . - pubmed
This abnormal fibrinogen supports adenosine diphosphate - induced platelet aggregation in a normal manner . - pubmed
The studies presented herein define time - dependent modifications of LDL constituents caused by CuSO4 - catalyzed oxidation . - pubmed
Measurement of the cholesterol content of oxidized LDL by the cholesterol esterase - oxidase assay was found to be inaccurate . - pubmed
Macrophage modification of LDL also resulted in the generation of oxysterols . - pubmed
Since incubation of macrophages with oxidized LDL but not native LDL resulted in the accumulation of oxysterols , this could account for some of the toxic and metabolic effects of oxidized LDL on cells . - pubmed
The 3H - product was isolated by thin - layer chromatography , and 1 - radyl-2 [ 3H ] , 3 - diacetylglycerols were prepared by phospholipase C digestion and subsequent acetylation at the sn-3 position . - pubmed
( 1 ) SPECULATION : Low levels of auxin activate cellulase ( by inducing H(+)-secretion ? ) - pubmed
( 2 ) SPECULATION : Moderate levels of auxin activate cellulase further ; the enzyme generates small amounts ( nM ) of oligosaccharides , some of which are growth - inhibitory . - pubmed
( 3 ) SPECULATION : Higher levels of auxin induce cellulase synthesis leading to the generation of higher concentrations of oligosaccharides that can mimic auxin . - pubmed
( 4 ) SPECULATION : Very high levels of auxin induce excessive levels of cellulase , and therefore of oligosaccharides . - pubmed
The following parameters were measured daily : pH , pO2 , pCO2 , HCO3 , glucose , lactic acid , lactic dehydrogenase , cellular ATP and platelet aggregation induced by different agents ( collagen and ADP ) . - pubmed
A marked increase in pO2 , conjugated dienes , malonyldialdehyde , lactic acid and lactic dehydrogenase activity was observed during the preservation . - pubmed
pCO2 , cyclooxygenase activity , vitamin E , coenzyme Q10 , bicarbonate and glucose showed a rapid fall . - pubmed
Fractionation of this high salt extract by sucrose density gradient centrifugation yielded a single peak of ATPase activity with an apparent sedimentation coefficient of 19 S. Electrophoretic analysis showed that this 19 S particle was composed of two heavy chains ( termed alpha and beta ; Mr 430,000 and 415,000 , respectively ) , five intermediate molecular weight chains ( IC1 - IC5 ; Mr 85,000 , 73,000 , 65,000 , 63,000 , and 57,000 ) , and six light chains ( LC1 - LC6 ; Mr 22,000 - 6,000 ) . - pubmed
The 19 S particle had a specific MgATPase activity of 1.1 + /- 0.3 mumol of phosphate released / min / mg and exhibited an apparent Km for MgATP2- of 40 + /- 16 microM. - pubmed
The Mg - ATPase activity was inhibited by vanadate , but not by ouabain or oligomycin , and exhibited a high activity between pH 7.0 and 10.0 with a maximum at pH 9.0 - 9.5 . - pubmed
ATP was the preferred nucleotide , although GTP and CTP ( but not ITP ) did interact with the dynein to a minor extent . - pubmed
The tricarboxylic acid cycle , glycolysis , gluconeogenesis , hepatic ketogenesis , and metabolism of ethanol are some of the metabolic processes amenable to 13C MRS . - pubmed
Carbon-13 MRS offers potential as a new investigative probe into organ physiology . - pubmed
The lowest effects in the cell - mediated experiments were seen with 7- , 8- , 9- and 10 - MBP , 2 - MBA , and 3 - MBA . - pubmed
On the other hand , among all the compounds tested 7,12 - dimethylbenz ( a ) anthracene , 12 - MBA , and 11 - MBP , which have the methyl group attached in the bay - region position opposite the terminal benzo ring , showed the highest mutagenic efficacies in the cell - mediated test , as compared to those observed in the homogenate - mediated test . - pubmed
When tested for melanophore pigment - dispersing activity in destalked Uca , [ Glu3 ] alpha - PDH was found to be 325 % more potent than alpha - PDH . - pubmed
Binding affinities were measured by the competitive displacement by bile acids of 1 - anilino-8 - naphthalene sulfonate ( ANS ) bound to the proteins and , in some cases , by direct methods of flow dialysis and equilibrium dialysis . - pubmed
Studies with specifically labeled ( 2H ) NAD ( P ) H and 2H2O and of the enzymes involved . - pubmed
Dihydrofolate reductase catalyzed the transfer of the pro - R hydrogen of NAD ( P ) H during the reduction of 7,8 - dihydrobiopterin to tetrahydrobiopterin . - pubmed
Sepiapterin reductase catalyzed the transfer of the pro - S hydrogen of NADPH during the reduction of sepiapterin to 7,8 - dihydrobiopterin . - pubmed
These results suggest that dihydrofolate reductase is not involved in the biosynthesis of tetrahydrobiopterin from dihydroneopterin triphosphate . - pubmed
Our results suggest that the reduction of 6 - pyruvoyl - tetrahydropterin might be catalyzed by sepiapterin reductase . - pubmed
Proceedings : Effects of phenobarbitone on glucose transport and membrane ATP - ase activities of cerebral cortex . - pubmed
35 C1 NMR studies of the active site zinc of horse liver alcohol dehydrogenase . - pubmed
Embryonic chick intestine in organ culture : interaction of adenylate cyclase system and vitamin D3 - mediated calcium absorptive mechanism . - pubmed
Protein kinase and cyclic 3 ' , 5 '- AMP : significance of binding and activation constants . - pubmed
Ribonucleotides and ribonucleosides as methyl acceptors for S - adenosylmethionine : ( amino - and thio -) purine methyl - transferases . - pubmed
In contrast , protamine sulfate enhanced the specific binding of 125I - EGF by increasing the apparent number of EGF receptors on the cell surface . - pubmed
The increase in 125I - EGF receptor binding was not prevented by cycloheximide nor by actinomycin D. - pubmed
Protamine II ( MW approximately 4,800 daltons ) was equally active ( half maximum inhibition concentration approximately 0.4 microM ) ; protamine IV ( MW approximately 3,300 daltons ) was substantially less active ( half maximum inhibition concentration approximately 2.8 microM ) . - pubmed
These investigations have extended previous observations that protamine sulfate is a potent inhibitor of PDGF binding and establish that protamine sulfate blocks PDGF binding at the physiological receptor , preventing PDGF initiated biological activities . - pubmed
The activity of the processing protease in purified brush border membranes was examined using endogenous substrates labeled with [ 3H ] fucose and [ 35S ] methionine . - pubmed
This fraction is enriched 5 - fold in the plasma membrane marker enzymes adenyl cyclase ( EC 4.6.1.1 ) and ouabain - inhibitable , ( Na + + K +)- dependent adenosine triphosphatase ( EC 3.6.1.3 ) . - pubmed
It is also enriched up to 14 - fold in iodinated surface proteins , and up to 4 - fold in ( 3H - mannose - labeled glycoproteins , of which the major variable surface coat glycoprotein is the main constituent . - pubmed
Several high molecular weight surface glycopeptides have been selectively investigated and partially characterized by a combination of metabolic labeling with [ 3H ] mannose , lactoperoxidase - catalyzed surface iodination , and affinity chromatography on Con A - Sepharose . - pubmed
Immunochemical identification of normal and variant hemoglobins after electrophoretic separation and transfer to nitrocellulose membranes . - pubmed
Renal excretion of calcium may have been impaired by renal dysfunction resulting in hypercalcaemia and suppression of plasma PTH and 1,25 ( OH ) 2D3 levels . - pubmed
Serum calcium and serum phosphorus were reduced 3 h after the daily subcutaneous CT injection ( 3 MRC - U / kg body weight ) , whereas a rebound increase in the serum levels of both minerals was observed at 24 hours after the injection . - pubmed
Following an early transient increase , the incorporation of strontium-85 into the fractured bones was impaired after 30 days of CT treatment . - pubmed
The effects of aspirin on cataract formation may result from 1 ) lowering of plasma tryptophan levels and increased excretion of tryptophan metabolites , 2 ) inhibition of aldose reductase and sorbitol formation in the diabetic lens , 3 ) inhibition of tryptophan or kynurenine binding to lens protein . - pubmed
Effects of targeted neuronal nitric oxide synthase gene disruption and nitroG - L - arginine methylester on the threshold for isoflurane anesthesia . - pubmed
Nonselective inhibition of nitric oxide synthase by nitroG - L - arginine methylester ( L - NAME ) reduces the minimum alveolar concentration of halothane anesthesia . - pubmed
Closure of the first spinal reflex are is established within 2 days and follows an antidromic pattern as related to the physiological spread of nerve impulses : synapses on motoneuronal somata and primary dendrites in the basal plate appear first and are followed by synapses in the alar plate , between dorsal root axon collaterals and somata of borderline ( commissural / association ) neurons . - pubmed
In batch fermentation studies , aad expression restored butanol formation ( 84 mM ) in mutant M5 without any acetone formation or any significant increase in ethanol production . - pubmed
The results demonstrate that in this case three doses of piperacillin have a higher efficacy than one dose in the prevention of febrile complications and post - operative infections . - pubmed
The antiaversive effects of morphine result from its action on mu receptors and the aversive ones may be associated with its action on other opiate receptors . - pubmed
RESULTS : IL-1 beta had no immediate effect on the basal or stimulated release of ACh . - pubmed
A marked suppression of ACh release was observed in tissues that had been preincubated with IL-1 beta for 60 minutes or more . - pubmed
The IL-1 beta effect was also blocked by cycloheximide and was spontaneously reversible after 60 minutes . - pubmed
CONCLUSION : It was concluded that IL-1 beta suppresses ACh release via the formation and release of a protein mediator that could be another cytokine , including IL-1 . - pubmed
BACKGROUND : We have recently described the association between the IgE antibody response to Ole e I ( the major antigen from olive tree pollen ) and the DR7 - DQ2 haplotype in a Spanish population . - pubmed
OBJECTIVE AND METHODS : Due to the linkage disequilibrium between DR7 and DQ2 , and thus the difficult distinction between the role of these two antigens in the T - cell activation response , we decided to solve this question by two approaches : 1 . - pubmed
Bronchial responsiveness to methacholine decreased significantly only after 6 wk of treatment with FP ( p & lt ; 0.05 ) . - pubmed
The effects on cholesterol were the result of a decrease in LDL- and VLDL - cholesterol without any modification of HDL - cholesterol . - pubmed
A synthetic transposon was constructed containing an ori , an ampicillin resistance ( Ampr ) gene , a multi - cloning site ( MCS ) and two hyperactive end sequences . - pubmed
The NOS antagonist , nitro - L - arginine ( 300 microM ) inhibited neurogenic relaxations in control and extrinsically - denervated tissues . - pubmed
Capsaicin- but not 6 - OHDA - treatment mimicked the effects of extrinsic denervation on NOS - ir and neurogenic relaxations of the longitudinal muscle . - pubmed
Troglitazone ( 1 - 20 microM ) dose - dependently inhibited both spontaneous and insulin - stimulated ET-1 secretion . - pubmed
This inhibitory effect of troglitazone was associated with reduced ET-1 mRNA levels . - pubmed
Addition of indomethacin ( 100 microM ) or Nw - nitro - l - arginine methyl ester ( 1 mM ) and downregulation of protein kinase C by prolonged pretreatment of the cells with a phorbol ester , 12 - O - tetradecanoylphorbol 13 - acetate , did not affect the inhibitory effect of troglitazone at concentrations up to 10 microM. - pubmed
Other PPARgamma ligands , pioglitazone ( 1 - 10 microM ) and 15 - deoxy - delta 12 , 14 - prostaglandin J2 ( 1 - 10 microM ) , but not a PPARalpha ligand , bezafibrate ( 1 - 10 microM ) , dose - dependently suppressed spontaneous ET-1 secretion from bVECs . - pubmed
These results , taken together , suggest that troglitazone inhibits ET-1 mRNA expression and secretion in bVECs possibly through activation of PPARgamma . - pubmed
This inhibition may contribute to the hypotensive effect of troglitazone in insulin - resistant subjects . - pubmed
A synthetic peptide of 20 amino acids from the putative binding site competed with in vitro synthesized NSP1 for binding to liposomes containing phosphatidylserine . - pubmed
DCM was identified by headspace gas chromatography - mass spectrometry ( GC - MS ) . - pubmed
Interestingly , coadministration of the vasodilator and the nitric oxide ( NO ) donor nitroglycerin not only prevented the increase of CPP and LVEDP , but also uncovered a slight positive inotropic effect of ET-1 in LVH hearts . - pubmed
Physical interactions between phospholamban and sarco ( endo ) plasmic reticulum Ca2 + - ATPases are dissociated by elevated Ca2 + , but not by phospholamban phosphorylation , vanadate , or thapsigargin , and are enhanced by ATP . - pubmed
Because the 1D11 antibody relieves inhibitory interaction between the two proteins , it was of interest to determine whether PLN phosphorylation or elevation of Ca ( 2 + ) , which also relieves inhibitory interactions between PLN and SERCA , would disrupt physical interactions . - pubmed
Co - immunoprecipitation was measured in the presence of increasing concentrations of Ca ( 2 + ) or after phosphorylation of PLN by protein kinase A. - pubmed
Physical interactions were dissociated by elevated Ca ( 2 + ) but not by PLN phosphorylation . - pubmed
The addition of ATP enhanced interactions between PLN and SERCA . - pubmed
The further addition of vanadate and thapsigargin , both of which stabilize the E(2 ) conformation , did not diminish binding of PLN to SERCA . - pubmed
These data suggest that physical interactions between PLN and SERCA are stable when SERCA is in the Ca ( 2 +)-free E(2 ) conformation but not when it is in the E(1 ) conformation and that phosphorylation of PLN does not dissociate physical interactions between PLN and SERCA . - pubmed
Even though the ubiquitin cross - reactivity was transiently lost from the sperm mitochondria during epididymal passage , likely as a result of disulfide bond cross - linking , it was restored and amplified after fertilization . - pubmed
Serial blood samples were collected 24 h post - dose to measure fluticasone propionate plasma concentrations . - pubmed
Similar or lower fluticasone propionate systemic exposure was observed with the HFA 134a pMDIs compared to the corresponding CFC inhalers . - pubmed
Therefore , the fluticasone propionate HFA 134a pMDI is an acceptable pharmaceutical alternative to the current CFC pMDI , producing similar lung deposition and no increase in systemic exposure at microgram equivalent doses . - pubmed
During the chronic phase of inflammation , in either model , CP reduced the elevated serum and exudate ( local ) LTB ( 4 ) and IL-6 levels . - pubmed
The antiarthritic effect of CP was also accompanied by augmentation of serum level of protein thiols , with reduction or normalization of elevated systemic and/or local levels of lipid peroxide , superoxide dismutase , and glutathione . - pubmed
The presence of erm genes coding for ribosomal methylases and of mefE - like genes responsible for macrolide efflux was screened by a multiplex polymerase chain reaction and confirmed by DNA / DNA hybridization . - pubmed
Of the 110 strains studied , 108 were cross - resistant to erythromycin , spiramycin and clindamycin , including 105 strains containing ermB - related genes and three strains that contained a combination of ermB- and mefE - related genes . - pubmed
Two strains apparently susceptible to clindamycin but resistant to spiramycin also contained ermB - related genes . - pubmed
No strain was resistant to erythromycin alone or contained only a mef - like gene . - pubmed
In this study , we found that Dex inhibited both spontaneous and TNF - induced activation of cPLA ( 2 ) . - pubmed
Dex had no direct effect on cellular cPLA ( 2 ) levels , but facilitated cPLA ( 2 ) degradation upon subsequent stimulation of cells with TNF . - pubmed
In addition , Dex increased synthesis of the endogenous cPLA ( 2 ) inhibitor lipocortin 1 ( LC1 ) . - pubmed
An antisense oligonucleotide to LC1 could completely abrogate Dex - induced resistance to the cytotoxic action of TNF . - pubmed
Compared with the control group , MB reduced the requirement for norepinephrine , epinephrine , and dopamine by as much as 87 % , 81 % , and 40 % , respectively . - pubmed
MB also reduced the body temperature and the plasma concentration of nitrates / nitrites . - pubmed
Leukocytes and organ function variables such as bilirubin , alanine aminotransferase , urea , and creatinine were not significantly affected . - pubmed
Results from this study indicate that the monoclonal antibody - based ELISA could be useful for a sensitive , quantitative , or qualitative ractopamine screening assay . - pubmed
Regulation of ryanodine receptors via macromolecular complexes : a novel role for leucine / isoleucine zippers . - pubmed
The mechanism underlying this loss of cardiac EC coupling gain involves altered regulation of the cardiac ryanodine receptor ( RyR2 ) , the major sarcoplasmic reticulum Ca ( 2 + ) release channel in the heart . - pubmed
The targeting of PKA , PP1 , and PP2A to RyR2 is dependent on the binding of targeting proteins to the channel via highly conserved leucine / isoleucine zippers ( LIZs ) . - pubmed
We report for the first time the mucicarmine - staining pattern of this tumor as well as immunohistochemistry directed against cytokeratin 7 , the estrogen receptor , and the progesterone receptor . - pubmed
These include argyrophilia , as detected by silver degeneration stains , and an elevation in glial fibrillary acidic protein ( GFAP ) , a marker of reactive gliosis in response to injury , as well as a long - term decrease in tyrosine hydroxylase ( TH ) protein levels . - pubmed
The SK3 subunit of small conductance Ca2 + -activated K + channels interacts with both SK1 and SK2 subunits in a heterologous expression system . - pubmed
When rSK3VK was co - expressed with rSK2 the resulting currents had an intermediate sensitivity to UCL 1848 ( IC50 of approximately 5 nM compared with 120 pM for rSK2 and & gt;300 nM for rSK3VK ) , suggesting that rSK3 and rSK2 can form functional heteromeric channels . - pubmed
To determine whether these assemblies were functional we created rSK3VF , an rSK3 mutant with an enhanced affinity for tetraethylammonium chloride ( TEA ) ( IC50 of 0.3 mM ) . - pubmed
Co - transfection of rSK3VF and hSK1 produced currents with a sensitivity to TEA not different from that of hSK1 alone ( IC50 approximately 15 mM ) . - pubmed
At meiotic prophase , the meiocyte walls were additionally marked by the appearance of poorly methyl - esterified domains of homogalacturonan and of ( 1 - ->4)-beta - Gal residues , detected by JIM5 and LM5 . - pubmed
PURPOSE : RSR13 , an allosteric modifier of hemoglobin , reduces hemoglobin - oxygen binding affinity facilitating oxygen release from hemoglobin , resulting in increases in tissue pO(2 ) . - pubmed
The purpose of this study was noninvasively to monitor the time course and effect of RSR13 on tumor oxygenation , directly using in vivo electron paramagnetic resonance ( EPR oximetry ) , and indirectly using blood oxygen level dependent magnetic resonance imaging ( BOLD MRI ) . - pubmed
Then , for 6 consecutive days and after an initial baseline EPR measurement , RSR13 ( 150 mg / kg ) or vehicle ( same volume ) was injected intraperitoneally , and measurements of intratumoral oxygen were made at 10 - min intervals for the next 60 min . - pubmed
RESULTS : Based on EPR measurements , RSR13 produced statistically significant temporal increases in tumor pO(2 ) over the 60 - min time course , which reached a maximum at 35 - 43 min postdose . - pubmed
There was little change in R(2 ) * , indicating that the RSR13 had minimal detectable effects on total deoxyhemoglobin and hemoglobin - oxygen saturation . - pubmed
The dynamics of tumor pO(2 ) after RSR13 administration may be useful for the design of clinical protocols using allosteric hemoglobin effectors . - pubmed
Use of fatty acid RPMI 1640 media for testing susceptibilities of eight Malassezia species to the new triazole posaconazole and to six established antifungal agents by a modified NCCLS M27 - A2 microdilution method and Etest . - pubmed
dermatis ( 2 isolates ) against amphotericin B , ketoconazole , itraconazole , fluconazole , voriconazole , terbinafine , and posaconazole by BMD and Etest . - pubmed
Because of higher amphotericin B MICs provided by Etest for strains also displaying high BMD MICs ( & gt;/=1 microg / ml ) , agreement was poorer . - pubmed
The changes in the rest energy expenditure ( REE ) , non - protein respiratory quotient ( NPRQ ) , glucose metabolism , nitrogen balance as well as plasma levels of endocrines and inflammatory mediators were determined before and after the administration of rhGH . - pubmed
The plasma levels of PGE2 , TXB2 , TNF - alpha and IL-6 significantly decreased and insulin , rhGH and IGF-1 obviously increased when compared with those in group B. - pubmed
Tumor size , histology , grade , lymph node status , estrogen ( ER ) and progesterone receptor ( PgR ) status , HER-2 expression , peritumoral vascular invasion ( PVI ) , Ki-67 expression , extensive in situ component ( EIC ) , and multifocality between the two groups were analyzed . - pubmed
Heat - induced gelation ( 80 degrees C , 30 min or 85 degrees C , 60 min ) of whey protein concentrate ( WPC ) solutions was studied using transmission electron microscopy ( TEM ) , dynamic rheology and polyacrylamide gel electrophoresis ( PAGE ) . - pubmed
TEM analyses showed that the SDS - gels had finer aggregate structure ( approximately equal to 10 nm ) than the control gels ( approximately equal to 100 nm ) , whereas the DTT - gels had a more particulate structure ( approximately equal to 200 to 300 nm ) . - pubmed
In both the SDS - solutions and the DTT - solutions , the denatured whey protein molecules were in the form of monomers or small aggregates . - pubmed
Results indicated that the mixture of MC / CP performed well as a biofilter media and the ammonia removal efficiency was 100 % for the filter depth of 500 mm . - pubmed
Asymmetric dimethylarginine : a molecule responsible for the coexistence of insulin resistance and atherosclerosis via dual nitric oxide synthase inhibition . - pubmed
Asymmetric dimethylarginine ( ADMA ) has been recently identified as the major endogenous inhibitor of soluble nitric oxide synthase . - pubmed
Here , we propose that ADMA causes insulin resistance by the inhibition of the neuronal isoform of nitric oxide synthase , while the simultaneously observed atherosclerosis is the consequence of endothelial nitric oxide synthase ( NOS ) inhibition . - pubmed
In these models , loss of hepatic nitric oxide productions is presumed to hinder a very potent insulin sensitizing mechanism referred to as meal induced sensitization that is anatomically linked to the nitrergic fibers of the anterior hepatic plexus . - pubmed
In our hypothesis , we suggest that ADMA by inhibiting both the neuronal and the endothelial forms of NOS , results both in insulin resistance and in accelerated atherosclerosis , therefore ADMA is the molecule responsible for the coexistence of these two conditions . - pubmed
HIF-1 , a hypoxia inducible transcription factor , plays a pivotal role in the cellular response to hypoxia by activating genes involved in glucose metabolism , vascular remodeling , and erythropoiesis . - pubmed
Further studies revealed that small molecule inhibitors of p38 ( SB203580 ) and MEK / ERK ( U1026 ) further reduced the remaining effect of TGF - beta1 in Smad3 - deficient fibroblasts . - pubmed
To move from finger to ear concentrations , using the Lactate Pro , modelling analysis suggested a regression equation of Y = 0.827x + 0.769 adjustment for pre climb samples and Y = 0.955x + 0.566 for post climb concentrations . - pubmed
Under conditions of oxygen and glucose deprivation , translocation rates of both compounds increased ; however , unlike rtPA , desmoteplase transport remained LRP - dependent . - pubmed
The replacement of the amino acid residue A43 , located in the second shell of riboflavin synthase active center , in the recombinant N - terminal domain dimer reduces the affinity for 6,7 - dimethyl-8 - ribityllumazine . - pubmed
The mutation of the amino acid residue C48 forming part of activity cavity of the enzyme causes significant ( 19 ) F NMR chemical shift modulation of trifluoromethyl derivatives of 6,7 - dimethyl-8 - ribityllumazine in complex with the protein , while substitution of A43 results in smaller chemical shift changes . - pubmed
Observations on the influence of exogenous gonadotrophins and cloprostenol on ovulation in gilts . - pubmed
We studied the effects of gonadotrophins and prostaglandin ( PG ) F ( 2alpha ) on ovulation in gilts . - pubmed
At 34 and 36 h after hCG , gilts received injections of either 500 mug or 175 mug PGF ( 2alpha ) ( cloprostenol ) , or had no injections . - pubmed
Laparotomies were performed at 36 h ( cloprostenol gilts ) or 38 h ( controls ) after hCG injection . - pubmed
AIM : To investigate the hypothesis that the protective effects of curcumin in hepatic warm ischemia / reperfusion ( I / R ) injury are associated with increasing heat shock protein 70 ( Hsp70 ) expression and antioxidant enzyme activity . - pubmed
A higher rate of apoptosis was observed in I / R group than in C + I / R group , and a significant increase of MDA , NO ( 2 )(- ) + NO ( 3 )(- ) and MPO level in liver tissues and serum transaminase concentration was also observed in I / R group compared to C + I / R group . - pubmed
Curcumin also decreased the activity of inducible NO synthase ( iNOS ) in liver after reperfusion , but had no effect on the level of endothelial NO synthase ( eNOS ) after reperfusion in liver . - pubmed
Dermal calcium loss during exercise was estimated using a patch collection technique to examine calcium loss as a potential mediator of changes in BMD . - pubmed
There were no differences in changes in BMD between the calcium supplementation groups . - pubmed
Higher dermal calcium losses were associated with lower baseline BMD values at the total hip , neck , and shaft ( all p & lt ; 0.05 ) , but were not significantly associated with changes in BMD . - pubmed
For instance , endonuclease III ( Nth ) does not excise thymine glycol ( Tg ) when it is part of either tandem lesion . - pubmed
The tandem lesion is also incised by the bacterial nucleotide excision repair system UvrABC with almost the same efficiency as an isolated Tg . - pubmed
Rapid and sensitive HPLC - MS / MS method for pharmacokinetic assessment of ribavirin in healthy Chinese . - pubmed
Colloidal gold nanoparticles ( AuNPs ) were directly assembled onto a surface of SPR Au chip via 2 - aminoethanethiol for the enhancement of sensitivity as a label - free detection system . - pubmed
SPR analyses indicated the signal increases for successive binding of hIgG and anti - hIgG onto the GBP - ProA layered AuNPs - assembled chip were higher ( about 92 and 30 % , respectively ) than that onto the identically treated bare chip . - pubmed
These results demonstrate the direct assembly of AuNPs onto a SPR chip could enhance the signal in biomolecular interaction events , and the GBP - ProA protein could be a valuable crosslinker for simple and oriented immobilization of antibodies onto Au chip surfaces without any surface chemical modification . - pubmed
Further , we analysed LeExt1::beta - glucuronidase reporter gene for root hair formation and found increasing expression of LeExt1 in the CO - exposed root hairs . - pubmed
Several protein growth factors can induce endothelial cell proliferation and angiogenesis , through signal transduction cascades that result in the production of several inflammatory mediators and lipid second messengers such as prostaglandins and Platelet Activating Factor ( PAF ) . - pubmed
S - Nitrosothiols ( RSNOs ) represent an important class of post - translational modifications that preserve and amplify the actions of nitric oxide and regulate enzyme activity . - pubmed
Herein , we report the reaction of S - nitrosated cysteine , glutathione , and a mutated C165S alkyl hydroperoxide reductase with the water - soluble phosphine tris ( 4,6 - dimethyl-3 - sulfonatophenyl ) phosphine trisodium salt hydrate ( TXPTS ) . - pubmed
A combination of NMR and MS techniques reveals that these reactions produce covalent S - alkylphosphonium ion adducts ( with S - P (+ ) connectivity ) , TXPTS oxide , and a TXPTS - derived aza - ylide . - pubmed
In conclusion , the present work reveals an unexpected function of dMyc in the systemic control of growth in response to steroid hormone signaling . - pubmed
In diestrus , with the rise of progesterone , four ( I - IV ) distinct morphological changes were evident . - pubmed
Mortality , serum levels of markers of hepatorenal injuries , cytokines , insulin , glucose and lipid derivatives , and the hepatic signaling pathway regulating gluconeogenesis were investigated . - pubmed
Consistently , apart from the case of 3 ( 3 + ) , which shows some degree of delocalization , all of the oxidized forms of 3(2 + ) , 4(2 + ) , and 5(2 + ) can be described as class II , localized mixed - valent species , in agreement with the electrochemical data showing two close oxidation potentials around 1 V vs FeCp *( 2 ) . - pubmed
Atorvastatin is a selective HMG - CoA reductase competitive inhibitor , used for the treatment of hyperlipidaemia . - pubmed
Hence there is more possibility of atorvastatin for inhibition of metabolism of glyburide , by both CYP 2C9 and 3A4 . - pubmed
The results of this study revealed that atorvastatin led to the PK / PD changes have been due to glyburide increased bioavailability , decrease volume of distribution , and/or decrease total clearance may be due to the inhibition of cytochrome P450 metobolic system . - pubmed
The studied subjects were determined in plasma : lipid parameters ( T - C , HDL - C , TG , LDL - C ) , total antioxidant status , TAS ( Randox , Statfax 1904 Plus ) and thiobarbituric acid - reactive substances , TBARS ( Sigma , UV-160A Shimadzu ) as lipid peroxidation products . - pubmed
NGT - P group comparing with NGT - N group presented higher concentration of fasting glucose , T - C , LDL - C , and TBARS ( 8.43 + /- 5.24 vs 4.81 + /- 2.58 nmol/ l ) . - pubmed
Media were analyzed for ovarian steroids and anti - mullerian hormone ( AMH ) . - pubmed
In the presence of fetuin at 5 % O2 , follicles had the highest survival rate if cultured with high or medium FSH , whereas follicles grew to larger diameters at Week 5 in low FSH . - pubmed
High FSH stimulated steroid production by growing follicles , and steroidogenesis by follicles cultured with low FSH was promoted by LH . - pubmed
AMH biosynthesis was elevated with high compared with low FSH and for longer under 5 % O2 than under 20 % O2 . - pubmed
Although the CFP signal was equally distributed in both cells of a germinating conidium , reactive oxygen species accumulated only in the cell that produced a germ tube , indicating that CgRac1 was active only in the germinating cell . - pubmed
Here , we demonstrate the efficacy of a strong base , 1,8 - bis ( dimethylamino ) naphthalene ( DMAN ; proton sponge ) , as a novel matrix for MALDI - time - of - flight ( TOF ) MS analysis of whole cell bacteria . - pubmed
These results indicate that DMAN represents a suitable matrix for MALDI - TOF MS analysis of mixtures of complex lipids as the intact membranes of microorganisms . - pubmed
The tensor components were determined by analyzing the configuration of phalloidin stained F - actin fibers in the media layer of pulmonary arteries . - pubmed
[ Effect of local basic fibroblast growth factor and 5 - fluorouracil on accelerating healing and preventing tendon adhesion after flexor tendon repair ] . - pubmed
CONCLUSION : Local single - use bFGF and 5 - FU can not only effectively promote the healing of flexor tendon , but also significantly reduce tendon adhesion . - pubmed
Relation of sex and estrogen therapy to serum fibroblast growth factor 23 , serum phosphorus , and urine phosphorus : the Heart and Soul Study . - pubmed
Fibroblast growth factor 23 ( FGF-23 ) is a hormone originating from bone that regulates urine phosphorus excretion . - pubmed
Whether sex or estrogen therapy is associated with different FGF-23 levels is unknown . - pubmed
OUTCOMES : Serum phosphorus , tubular maximum reabsorption of phosphorus indexed to glomerular filtration rate ( TMP / GFR ) , and plasma FGF-23 concentrations . - pubmed
Mean FGF-23 levels were 68.7 ( 95 % CI , 59.7 - 79.0 ) relative units ( RU)/mL in non - estrogen - using women , 43.8 ( 95 % CI , 41.2 - 46.5 ) RU / mL in men , and 45.1 ( 95 % CI , 35.2 - 57.4 ) RU / mL in women using estrogen in adjusted analysis ( P & lt ; 0.001 ) . - pubmed
In the context of prior literature , these data suggest that postmenopausal phosphorus retention may stimulate higher FGF-23 concentrations after menopause . - pubmed
The screen included height , weight , and systolic ( SBP ) and diastolic ( DBP ) blood pressure measurements plus a blood draw following an overnight fast for fasting glucose . - pubmed
INVESTIGATIONS : All approaches on the metabolic ward to control insulin resistance failed as well and finally blood glucose levels could only be stabilized with the aid of continous subcutaneous insulin infusion with more than 500 units per day . - pubmed
Heme Oxygenase-1 and breast cancer resistance protein protect against heme - induced toxicity . - pubmed
We postulated that overexpression of Heme Oxygenase-1 ( HO-1 ) and Breast Cancer Resistance Protein ( BCRP ) would protect against heme - induced cytotoxicity . - pubmed
HO-1 is a heme - degrading enzyme generating carbon monoxide , iron , and biliverdin / bilirubin , while BCRP is a heme efflux transporter . - pubmed
Heme - induced cell death was significantly attenuated when cells overexpressed HO-1 , BCRP , or both . - pubmed
The protective effects of HO-1 overexpression were most pronounced , while co - treatment with the HO - activity inhibitor tin mesoporphyrin reversed these protective effects . - pubmed
Also cells treated with the anti - oxidants N - acetylcysteine or HO - effector molecule bilirubin showed protection against heme insults , which may explain the increased protection by HO-1 compared to BCRP . - pubmed
In conclusion , both HO-1 and BCRP protect against heme - induced toxicity and may thus form novel therapeutic targets for heme - mediated pathologies . - pubmed
DESIGN : To evaluate neurobehavioral alterations following the development of DIO induced by an 8 - week high - fat diet ( HF ) exposure , striatal D2 - receptor density , DAT function and expression , extracellular dopamine concentrations , impulsivity , and motivation for high- and low - fat reinforcers were determined . - pubmed
To determine predictors of DIO , neurobehavioral antecedents including impulsivity , motivation for high - fat reinforcers , DAT function and extracellular dopamine were evaluated before the 8 - week HF exposure . - pubmed
METHODS : Striatal D2 - receptor density was determined by in vitro kinetic analysis of [ ( 3 ) H ] raclopride binding . - pubmed
DAT function was determined using in vitro kinetic analysis of [ ( 3 ) H ] dopamine uptake , methamphetamine - evoked [ ( 3 ) H ] dopamine overflow and no - net flux in vivo microdialysis . - pubmed
Activity was measured by monitoring the levels of aspartate aminotransferase ( AST ) , alanine aminotransferase ( ALT ) , alkaline phosphatase ( ALP ) and bilirubin , along with histo - pathological analysis . - pubmed
Herein a method is described for labeling mesenchymal stem cells ( MSC ) , in which the physical state of citrate - coated nanoparticles ( dispersed versus aggregated ) can be kinetically tuned through electrostatic and magnetic triggers , as monitored by diffusion light scattering in the extracellular medium and by optical and electronic microscopy in cells . - pubmed
RhoA - induced NFAT activation and fibronectin upregulation were both dependent on the calcium / calmodulin pathway and Rho kinase . - pubmed
RhoA activation induced a rise of intracellular calcium ion concentration ( [ Ca ( 2 +)]i ) , which was at least in part dependent on the transient receptor potential canonical 6 ( TRPC6 ) cation channel . - pubmed
The results indicate that RhoA activates NFAT by inducing a rise of [ Ca ( 2 +)]i in podocytes , which in turn contributes to fibronectin upregulation . - pubmed
We observed dysregulation of genes involved in bone remodeling ( osteoprotegerin , osteocalcin , osteopontin ) and vitamin D metabolism ( 7 - dehydrocholesterol reductase , Gc - globulin , Cyp2r1 , Cyp27a1 ) as well as alterations in calcium and vitamin D homeostasis . - pubmed
AIM : In paediatric dentistry , epinephrine may contribute to systemic and local side - effects . - pubmed
Interestingly , enkephalins and mu - opioid receptors ( mu - ORs ) are known for their role in ingestive behaviors and have previously been shown to regulate the excitability of MnPO neurons following a single Na (+ ) depletion . - pubmed
Interestingly , the subpopulation of Na (+ ) sensors responding to D - Ala(2 ) , N - MePhe ( 4 ) , Gly - ol - enkephalin ( DAMGO ) , a specific mu - OR agonist , represented 11 % of MnPO neurons after a single Na (+ ) depletion and the population doubled after three Na (+ ) depletions . - pubmed
The best fit multiple regression model ( Adjusted R2 : 0.494 , p = 0.020 ) included Plasma Glucose as an independent variable , and the squared value of cohesion score , depression score of HAD , Total calorie intake , Exercise time , Housekeeping time , and BMI were dependent variables . - pubmed
The potent metallo - beta - lactamase NDM-1 efficiently hydrolyses all classes of beta - lactam antibiotics ( penicillins , cephalosporins and carbapenems ) and is also associated with multiple determinants that enable the bacteria to become resistant to other antibiotic classes ( Nordmann et al . - pubmed
In the presence of NDM-1 other beta - lactamases may go unobserved because of the spectrum of activity of NDM-1 against all beta - lactam antibiotics . - pubmed
BACKGROUND : Angiotensin I - converting enzyme ( ACE ) has two functional N- and C - domain active centers that display differences in the metabolism of biologically - active peptides including the hemoregulatory tetrapeptide , Ac - SDKP , hydrolysed preferentially by the N domain active center . - pubmed
In silico analysis demonstrated S333W localized in the S1 pocket of the active site of the N domain with the bulky Trp adversely affecting binding of ACE substrates due to steric hindrance . - pubmed
CONCLUSIONS AND SIGNIFICANCE : A novel Ser333Trp ACE mutation results in dramatic changes in ACE kinetic properties and lowered clearance of Ac - SDKP . - pubmed
Wound bed preparation 2014 update : management of critical colonization with a gentian violet and methylene blue absorbent antibacterial dressing and elevated levels of matrix metalloproteases with an ovine collagen extracellular matrix dressing . - pubmed
Group RS ( n = 20 ) received USGRSB 0.5 mg kg(-1 ) 0.25 % bupivacaine per side in the posterior rectus sheath compartment . - pubmed
Pharmacological inhibitors of lysosomal degradation , such as chloroquine , increased AF1Q levels , whereas activators of CMA , including 6 - aminonicotinamide and nutrient starvation , decreased AF1Q levels . - pubmed
Using an amino acid deletion AF1Q mutation plasmid , we identified that AF1Q had a KFERQ - like motif which was recognized by HSPA8 for CMA - dependent proteolysis . - pubmed
INTRODUCTION : Edoxaban is an oral , once - daily direct factor Xa ( FXa ) inhibitor . - pubmed
Intrinsic FX activity was increased up to 20 % and 31 % of normal in the presence of edoxaban by rFVIIa and FEIBA , respectively . - pubmed
CONCLUSIONS : The findings of this ex vivo study suggest that rFVIIa and FEIBA rapidly reversed edoxaban - mediated anticoagulation effects based on PT and aPTT , but had minimal effect based on intrinsic FX activity . - pubmed
RESULTS : The consumption of probiotic yogurt caused significant decrease in LDL - c / high density lipoprotein cholesterol ( HDL - c ) ratio ( 3.13 + - 1.00 - 2.07 + - 0.71 , P = 0.016 ) . - pubmed
It was found that to achieve efficient nitrogen removal via partial nitrification - denitrification ( PND ) , " DO elbow " point must appear at the end of the last aeration period . - pubmed
There was a correlation between the ammonium oxidizing bacteria ( AOB)/nitrite oxidizing bacteria ( NOB ) ratio and the average DO concentrations in the last aeration periods ; when the average DO concentrations in the last aeration periods were lower than 4.86 mg / L , AOB became the dominant nitrifier population , which benefited nitrogen removal via PND . - pubmed
To accomplish efficient nitrogen removal via PND , the optimum aeration rate ( A , L air / min ) applied can be predicted according to the average organic loading rates based on mathematical equations developed in this study . - pubmed
He had a heterozygous germline CARD11 mutation causing a G123D amino acid substitution , which was demonstrated to induce NF - kappaB activation in unstimulated lymphocytes . - pubmed
And the results of Western blot showed that hesperetin caused an increase in the levels of cytosolic AIF , cytosolic Apaf-1 , cytosolic Cyt C , caspase-3 , caspase-9 and Bax and a decrease in that of Bcl-2 , mitochondrial AIF , mitochondrial Apaf-1 and mitochondrial Cyt C ( P & lt ; 0.05 ) . - pubmed
Nucleotide - binding oligomerization domain-2 ( NOD2 ) is an innate immune receptor that recognizes peptidoglycan - derived muramyl dipeptide from intracellular bacteria and triggers proinflammatory signals . - pubmed
Owing to their intrinsic estrogenic activity , at maximal concentrations , OH - TORE and OH - OSPE blocked the stimulatory effect of E2 by only 89 % compared to 100 % with ACOL . - pubmed
Concentration - response curves to phenylephrine ( PHE ) were obtained from aortic rings incubated with N ( G )- nitro - l - arginine methyl ester ( l - NAME ) , superoxide dismutase ( SOD ) , indomethacin , SC-560 , and NS-398 . - pubmed
Glycine augmented the sensitivity for PHE in the presence of l - NAME and SOD . - pubmed
Glycine increased the mRNA expression of eNOS and decreased the expression of COX-2 and TNF - alpha . - pubmed
Cell proliferation , mineralized calcium deposition , alkaline phosphatase ( ALP ) activity and apoptosis were assessed at various time - points . - pubmed
The 58 subjects were assessed during each phase of the study for clinical presentation of gingivitis and concurrently had plaque sampled for real - time polymerase chain reaction ( RTPCR ) microbiological characterization and salivary lavage samples for ' systems biology ' metabonomics assessment by 1H - NMR . - pubmed
OBJECTIVE : The purpose of this study was to investigate the pathogenic role of RyR - bound CaM in diastolic Ca2 + leakage from the sarcoplasmic reticulum and arrhythmogenesis in pressure - overloaded heart failure . - pubmed
The frequency of spontaneous Ca2 + transients in TAC cells during and after 1 - 5 Hz of field stimulation was 44 % , whereas it was significantly attenuated by HA - CaM but not with CaM. - pubmed
CONCLUSION : Several disorders in the RyR channel function characteristic of pressure - overloaded cells ( increased spontaneous Ca2 + leakage , delayed afterdepolarization , triggered activity , Ca2 + spark frequency , spontaneous Ca2 + transients ) are caused by deteriorated CaM binding to RyR2 . - pubmed
Twenty - eight SNPs associated with TGs , 39 SNPs associated with total cholesterol ( TC ) , 28 SNPs associated with LDL cholesterol ( LDL - C ) , and 56 SNPs associated with HDL cholesterol ( HDL - C ) were analyzed individually and combined into genetic risk scores ( GRSs ) . - pubmed
Cholesterol - dependent QS-21 endocytosis followed by lysosomal destabilization and Syk kinase activation were prerequisites for this response . - pubmed
OBJECTIVE : To assess whether type 2 inflammation , as measured by sputum eosinophils and fractional exhaled nitric oxide ( FeNO ) , is a feature of naturally occurring virus - induced exacerbations of asthma and whether TSLP is associated with this type 2 inflammation . - pubmed
In the framework of the VATO project , the model has been tested against two - year - long in situ measurements of 3H activity concentration monitored by IRSN in air , groundwater and grass , together with meteorological parameters , on a grass field plot located 2 km downwind of the AREVA NC La Hague nuclear reprocessing plant , as was done in the past for the evaluation of transfer of 14C in grass . - pubmed
Further experimental investigation will be undertaken by IRSN and EDF next year to better evaluate ( and properly model ) other aspects of tritium transfer where knowledge gaps have been identified in both experimental and modelling areas . - pubmed
The Waters BEH C18 column was used with a gradient mobile phase system of acetonitrile and water containing 0.1 % formic acid . - pubmed
NLRP4 is an essential negative regulator of fructose - induced cardiac injury in vitro and in vivo . - pubmed
Here , we illustrated that NLRP4 attenuates pro - inflammatory cytokines releasing , including Transforming growth factor ( TGF - beta1 ) , Tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1beta ( IL-1beta ) , interleukin-18 ( IL-18 ) and interleukin-6 ( IL-6 ) , in fructose - treated cardiac cells by means of RT - qPCR , and western blotting analysis . - pubmed
In vivo , high fructose feeding induced cardiac injury , accompanied with reduced expression of NLRP4 in heart tissue samples , indicating the possible role of NLRP4 in ameliorating heart injury . - pubmed
Dopamine D2 Receptors in the Paraventricular Thalamus Attenuate Cocaine Locomotor Sensitization . - pubmed
Alterations in thalamic dopamine ( DA ) or DA D2 receptors ( D2Rs ) have been measured in drug addiction and schizophrenia , but the relevance of thalamic D2Rs for behavior is largely unknown . - pubmed
We therefore hypothesise that bleomycin injections in combination with electroporation-'electrosclerotherapy ' ( EST ) , also known as ' electrochemotherapy'-could potentially be a novel alternative treatment option for CMs . - pubmed
Three regions of interest ( ROIs ) within the CM will be randomly allocated for treatment with ( A ) EST , ( B ) bleomycin sclerotherapy without electroporation and ( C ) no treatment . - pubmed
However , the proportion of somata containing M1R among cortical cellular types , and the precise intracellular localization of M1R remain unclear . - pubmed
M1R immunopositivity signals were found in the majority of the somata of both pyramidal neurons and inhibitory neurons . - pubmed
In pyramidal neurons , strong M1R immunopositivity signals were usually found throughout their somata and dendrites including spines . - pubmed
On the other hand , the signal strength of M1R immunopositivity in the somata of inhibitory neurons significantly varied . - pubmed
In PV neurons , M1R immunopositivity signals were preferentially distributed in somata . - pubmed
RESULTS : There is accumulating evidence of increased sensitivity to platinum - based therapy and poly - ADP - ribose polymerase inhibitors ( PARPi ) in BRCA - associated PDAC . - pubmed
Patients with BRCA - associated locally advanced PDAC can benefit from targeted therapy with PARPi ( olaparib ) as a second - line therapy after antimetabolite treatment failure . - pubmed
Patients with unresectable metastatic BRCA - positive PDAC may benefit from platinum - based therapy . - pubmed
The aim of the present study was to investigate whether Dicer , a key miRNA biogenesis enzyme , and miRNAs in macrophages are implicated in the anti - inflammatory effects of metformin . - pubmed
MATERIALS AND METHODS : Enzyme - linked immunosorbent assay and reverse transcription quantitative polymerase chain reaction were carried out to verify the anti - inflammatory effects of metformin . - pubmed
Western - blotting , enzyme - linked immunosorbent assay and reverse transcription quantitative polymerase chain reaction were used to examine the role Dicer and miRNAs play in the anti - inflammatory effects of metformin . - pubmed
RESULTS : In parallel with the suppression of interleukin-6 and tumor necrosis factor - alpha production in resting and lipopolysaccharide - stimulated macrophages , metformin could induce an increase in Dicer and most miRNAs . - pubmed
When Dicer was knocked down , the anti - inflammatory effects of metformin were significantly attenuated . - pubmed
Additionally , the upregulation of miRNA ( miR)-34a-5p and miR-125b-5p by metformin were also blunted in Dicer knockdown macrophages . - pubmed
CONCLUSIONS : Metformin might show anti - inflammatory effects in macrophages through the induction of Dicer and the subsequent upregulation of miR-34a-5p and miR-125b-5p . - pubmed
gamma - Thialysine versus Lysine : An Insight into the Epigenetic Methylation of Histones . - pubmed
Biomedicinally important histone lysine methyltransferases ( KMTs ) transfer a methyl group from S - adenosylmethionine to lysine residues in histones and other proteins . - pubmed
Here , we report comparative studies on epigenetic methylation of lysine and gamma - thialysine , the simplest cysteine - derived lysine analog , which can be introduced to histone peptides and histone proteins via site - specific bioconjugation - based cysteine alkylation . - pubmed
This work provides a molecular basis for the application of gamma - thialysine for biomolecular studies of intact histones and the nucleosome assembly . - pubmed
Examples using pipecolic acid for the construction of piperidine derivatives , as well as the one - pot synthesis of alpha - amino nitriles , are also shown . - pubmed
The Aurora kinases represent a group of serine / threonine kinases which are crucial regulators of mitosis . - pubmed
We report that AurkB undergoes acetylation in vitro by lysine acetyltransferases ( KATs ) belonging to different families , namely by p300 and Tip60 . - pubmed
The haploinsufficient tumor suppressor Tip60 acetylates two highly conserved lysine residues within the kinase domain of AurkB which not only impinges the protein stability but also its kinase activity . - pubmed
Anabolic steroids ( AS ) are synthetic testosterone - derivatives developed by the pharmaceutical industry to mimic testosterone biological effects . - pubmed
In conclusion , patients with lung adenocarcinoma harboring the EGFR S768I and G724S mutations appear less sensitive to icotinib than patients with sensitive EGFR . - pubmed
If APRT deficiency is recognized early , renal failure can be prevented , arrested or reversed in native kidney and in allograft by treatment with allopurinol , which inhibits xanthine oxidase and reduces 2,8 - DHA formation . - pubmed
Tunable absorber embedded with GST mediums and trilayer graphene strip microheaters . - pubmed
The absorber configuration exploits the Ge2Sb2Te5 ( GST ) phase changing medium , and the effects of different parametric and operational conditions on the absorption spectra were explored . - pubmed
A controlled clinical trial of streptokinase and heparin in the treatment of major pulmonary embolism . - pubmed
Streptokinase of heparin was given for 72 hours and pulmonary angiography was repeated . - pubmed
Acute respiratory and metabolic alkalosis significantly reduced serum potassium by about 26 % , while chronic furosemide treatment ( 1 mg / kg IV daily for 14 + /- 4 days ) significantly reduced both serum potassium concentration ( 4.16 + /- 0.31 to 3.27 + /- 0.14 mEq / L ) and skeletal muscle potassium content ( 80.9 + /- 5.6 to 58.7 + /- 4.1 mEq / kg ) . - pubmed
After rapid in vivo hydrolysis of the nafate pharmaceutical form to cefamandole ( II ) , the antibiotic was found to be very resistant to metabolic degradation in both species . - pubmed
LCAT activity is determined measuring the cholesterol esterification , and is expressed as micrograms of cholesterol esters formed per milliliter plasma per hour . - pubmed
A 2nd type of dietary constituent affecting the microsomal mixed - function oxidase system is added phenolic antioxidant , i.e. - pubmed
The cytochrome P-450 shows altered spectral characteristics , and the aryl hydrocarbon hydroxylase system of these microsomes has an increased sensitivity to inhibition by alpha - naphthoflavone . - pubmed
In addition , there is a decrease in binding of metabolites of benzo ( a ) pyrene to DNA upon incubation of these microsomes of induction of increased mixed function oxidase activity have shown that increased levels of activity protect against administration of chemical carcinogens . - pubmed
The thoracic accumulation of neutrophils labelled with 111Indium - oxine in response to infusion of platelet activating factor ( PAF , 18 ng / kg / min x 5 min , i.v . ) - pubmed
Pacemaker and spindle - like oscillations were unaffected by tetrodotoxin ( TTX ) or by selective blockade of NMDA , non - NMDA , GABAA , GABAB , nicotinic , muscarinic , alpha - and beta - noradrenergic receptors . - pubmed
Inspection of the amino acid sequence deduced from nucleotide sequence of lukS and that from S - component of leukocidin clarified that pre - matured S - component contains a typical signal sequence at the NH2 terminus . - pubmed
The 58 - kDa subunit of the microsomal transfer protein complex was identified as protein disulfide - isomerase ( PDI ) ( EC 5.3.4.1 ) by 1 ) a comparison of the amino - terminal sequence of PDI and the 58 - kDa subunit of the transfer protein , 2 ) a comparison of the reverse phase high performance liquid chromatography peptide maps of CNBr digests of PDI and the lipid transfer protein , 3 ) immunoprecipitation competition experiments in which PDI was found to compete with the lipid transfer protein for immunoprecipitation by the anti-58 - kDa polyclonal antibodies , 4 ) immunological cross - reactivity of the microsomal triglyceride transfer protein complex with polyclonal antibodies raised against PDI , and 5 ) the appearance of protein disulfide isomerase activity following the dissociation of purified microsomal transfer protein complex with guanidine HCl . - pubmed
Determination of serum rifampicin ( RFP ) after dose of the drug is often requested not only for the clinical criteria on its use , but also for the check of its adverse reactions . - pubmed
In comparative examination on each clinical use of 2 kinds of RFP commercial products : Rifadin and Aptesin , the authors had a chance of simultaneous determination of serum RFP by means of 3 different assay methods : solvent extraction method ( SE ) , biological activity method ( BA ) and liquid - chromatography method ( LC ) . - pubmed
The highest determinations were presented by the SE , which was devised as total assay method determining desacetyl RFP and 3 - formyl rifamycin SV besides free RFP . - pubmed
Thus the difference between both determinations by SE and LC was caused by RFP metabolites . - pubmed
Some attensions were called to the practical estimation of the serum RFP concentrations : Clear individual differences were found in the serum RFP concentrations after dose of the drug , and appeared to become clearer in the stage of its continuous administrations . - pubmed
It was shown that the difference in UDS rates between control and ACR fibroblast strains was not due to [ 3H ]- dThd catabolism and/or disappearance in the culture medium after a 34 - h labelling period . - pubmed
When the percent increases in N - acetoxy-2 - acetylaminofluorene ( NA - AAF)-induced UDS and in [ 3H ]- dThd incorporation in the presence of 10 mM hydroxyurea were compared between 24 h and 34 h incubation , the 13 ACR fibroblast lines were significantly lower compared to the corresponding values calculated for the seven control fibroblast strains . - pubmed
Binding of DT to asolectin or DL - alpha - dipalmitoylphosphatidylcholine - DL - alpha - dipalmitoylphosphatidic acid liposomes at pH 7.3 results in a 10 % increase in its alpha - helix content . - pubmed
At pH 4 , in the presence of liposomes , the secondary structure of DT is characterized by the appearance of a beta - sheet structure with strengthened hydrogen bonds which did not exist before pH lowering . - pubmed
However , the alpha - helix content of CB1 increases by 10 % , and polarization measurements indicate that the alpha - helices of CB1 at pH 4 are oriented parallel to the lipid acyl chains . - pubmed
The obtained data suggest the possibility of action of kynurenine and quinolinic acid via picrotoxin - sensitive subunits of the benzodiazepine - GABA - receptor - ionophore complex . - pubmed
The isometric contraction to phenylephrine was antagonised competitively by prazosin with a pA2 value of 9.45 , suggesting that the receptor is an alpha 1 - adrenoceptor . - pubmed
The drug succeeded in normalizing the cholesterol and triglyceride serum levels in acute as well as in chronic hyperlipaemia , and , in this connection , it provoked a significant increase of serum alpha - lipoproteins and a decrease of serum beta - lipoproteins , with a consequent improvement of the beta / alpha ratio . - pubmed
Analysis of plasma lipoproteins indicated that sucrose elevated VLDL and LDL when compared to lactose . - pubmed
The increase in lysozyme secretion by these cells , however , is markedly blunted suggesting that continued PMA treatment of differentiated cells is required for their secretion of lysozyme . - pubmed
There is a modest ( five - fold ) increase in lysozyme mRNA between 0 and 36 h of exposure to PMA , corresponding to the burst of lysozyme secretion by these cells . - pubmed
Wild - type cells labeled in their DNA with [ ( 3 ) H ] thymidine in the presence of TP produced small pieces of DNA ( 7 x 10(6 ) daltons ) , which corresponded in size to late replicative intermediates of discontinuous DNA synthesis . - pubmed
[ Phospholipid metabolism in pancreatitis -- effects of CDP - choline on phospholipase A ] . - pubmed
Osteoporosis , skin collagen , and androgen . - pubmed
Decreased body collagen may possibly be a primary defect in osteoporosis , while androgens may increase total skin collagen . - pubmed
Inhibition of histamine - induced nasal symptoms by the H1 antihistamine chlorpheniramine maleate : demonstration of topical effect . - pubmed
Progesterone or HPL treatments alone did not substantially influence LI . - pubmed
HPL treatment , but not progesterone or thyroxine treatments significantly enhanced the stimulatory effect of estrogen on LI . - pubmed
The mean plasma concentration - time curve ( Cp ) after intravenous ampicillin sodium administration was described biexponentially , as : Cp = 38.8 e -0.0268 t + 0.45 e -0.0058 t. Intramuscular injection , into the lateral neck , of Ampikel-20 and Polyflex resulted in 100 per cent bioavailabilities within 12 h post injection ( p.i . ) - pubmed
The specific activity of pig muscle D - glyceraldehyde-3 - phosphate dehydrogenase was found to be constant in the reverse reaction , with NADH and 1,3 - diphosphoglycerate as substrates , over the enzyme concentration range 10(-8 ) to 10(-4 ) M. The molecular weight of the covalent intermediate of the enzyme , 3 - phosphoglyceroyl - glyceraldehyde-3 - phosphate dehydrogenase , as measured by sedimentation techniques , proved constant ( 145 000 + /- 6 000 ) between 3 x 10(-5 ) M enzyme concentration . - pubmed
A Scatchard analysis showed that the rRNA - binding immunoglobulin is monoclonal and has a high affinity ( 10(9 ) liter / mole ) for the RNA antigen . - pubmed
It has been successful to locate the antigenic determinant of one of these hemoglobins within a defined amino acid sequence . - pubmed
Pretreatment of rings with indomethacin or adenosine or endothelin receptor antagonists had no effect on the hypoxia - mediated alterations . - pubmed
Monoclonal antibody 15A9 was shown to counteract GTP - induced inhibition , while 1F2 influenced the conformation of E1 , allowing two antibodies , which did not previously bind E1 , to bind to it . - pubmed
The vaccine consisted of the non - glycosylated FeLV envelope protein p45 , aluminium hydroxide and a saponin adjuvant . - pubmed
IGF - I treatment led to reduced fasting and stimulated ( glucose and/or L - arginine ) insulin and growth hormone secretion . - pubmed
Intravenous glucose tolerance was unaltered despite reduced insulin secretion . - pubmed
Resting energy expenditure and lipid oxidation were both elevated , while protein oxidation was reduced , and glucose turnover rates were unaltered on the fifth treatment day with IGF - I as compared to the control period . - pubmed
Moreover , insulin - stimulated oxidative and nonoxidative glucose disposal ( i.e. - pubmed
Thus , IGF - I treatment leads to marked changes in lipid and protein oxidation , whereas , at the dose used , carbohydrate metabolism remains unaltered in the face of reduced insulin levels and enhanced insulin sensitivity . - pubmed
TEM - EDS analyses indicated that Ca and P were the major elements , with small amounts of Mg . - pubmed
DHT administration in prepuberal castrates stimulated collagen synthesis and accumulation in the stroma of male accessory sex organs as well as the proliferation and differentiation of the glandular epithelium in these organs . - pubmed
Immune complex - stimulated neutrophil LTB4 production is dependent on beta 2 integrins . - pubmed
However , both genetically beta 2 - deficient PMN and PMN treated with the anti - beta 2 mAb IB4 subsequently detached from the IC - coated surfaces . - pubmed
Importantly , LTB4 production by PMN stimulated with fluid phase heat - aggregated IgG also required the beta 2 integrins , showing that the defect was not a simple consequence of abnormal adhesion . - pubmed
The initial rises in intracytoplasmic [ Ca2 +]i in response to aggregated IgG also were unaffected by inhibition of beta 2 integrins . - pubmed
Finally , LTB4 production by normal PMN adherent to ICs was inhibited by antibodies to FcRII , but not FcRIII , showing that FcRII ligation was required for this effect . - pubmed
Together these data identify a role for the beta 2 integrins in a signal transduction pathway leading to sustained adhesion and LTB4 production in response to IC . - pubmed
Anti - Thy 1.1 and anti - desmin antibodies recognizing glomerular mesangial cells and antibodies against O - acetyl GD3 and gp330 antigens of podocytes , as well as anti - thrombospondin and anti - cytokeratin antibodies identifying parietal epithelial cells failed to bind to our cells . - pubmed
Involvement of the CoA - independent transacylase demonstrated by analysis of the molecular species of platelet - activating factor . - pubmed
Consistent with the involvement of arachidonate - specific CoA - independent transacylase in the synthesis of platelet - activating factor ( PAF ; 1 - O - alkyl-2 - acetyl - GroPCho ) , 16:0 and 18:1 PAF species were formed upon antigen stimulation [ Joly , F. , Breton , M. , Wolf , C. , Ninio , E. & amp ; Colard , O. - pubmed
Consistent with alkyl - arachidonyl - GroPCho species content and acetyltransferase specificity , similar amounts of 16:0 and 18:1 PAF species were formed when mast cells were stimulated with antigen . - pubmed
More interestingly , addition of 16:0 lyso - PAF almost doubled the amount of 18:1 PAF content as compared to antigen alone , thus indicating that the lyso - PAF formed via the CoA - independent transacylase was significantly used for PAF synthesis , despite a large excess of exogenous lyso - PAF . - pubmed
However , PAF is preferentially synthesized through a mechanism involving the CoA - independent transacylase reaction . - pubmed
The decreased androgen / estrogen ratio , and the increased estrogen value could be responsible for an estrogen receptor mediated effect on the tubular epithelial cell proliferation , an event further potentiated by the action of regulatory peptides like epidermal growth factor ( EGF ) . - pubmed
Interactions of alpha - chymotrypsinogen A with alkylureas . - pubmed
Solvation of alpha - chymotrypsinogen A ( alpha - ctg A ) in aqueous urea , methylurea , N , N '- dimethylurea and ethylurea was studied by density measurements . - pubmed
UNLABELLED : Renal proximal tubular cells have been shown to express aromatic L - amino acid decarboxylase ( L - AAAD ) , which converts L - dopa into dopamine and 5 - hydroxytryptophan [ ( OH ) Trp ] into 5 - hydroxytryptamine ( 5 - HT ; serotonin ) . - pubmed
Because 5 - HT receptors have been demonstrated in proximal cells , we hypothesized that 5 - HT may act as an autocrine / paracrine modulator of proximal transport . - pubmed
5 - HT reversed parathyroid hormone ( PTH)-induced cAMP accumulation in a pertussis toxin - sensitive manner and reduced the PTH inhibition of P(i ) uptake without affecting the NaP ( i )- 4 mRNA level . - pubmed
The effects of 5 - HT on cAMP generation and Na - P ( i ) co - transport were reproduced by ( OH ) Trp , except in the presence of benserazide , and by L - propranolol and dihydroergotamine , two 5 - HT1B receptor agonists . - pubmed
Linear IgA bullous dermatosis after contact with sodium hypochlorite . - pubmed
A bifunctional enzyme , fructose-6 - phosphate 2 - kinase - fructose 2 , 6 - bisphosphatase , catalyzes synthesis and degradation of fructose 2 , 6 - bisphosphate . - pubmed
Mutants of basic residues , including Lys51 , Arg78 , Arg79 , Arg136 , Lys172 , and Arg193 , immediately around the active site of rat testis fructose 6 - P,2 - kinase were constructed , and their steady state kinetics , ATP binding , and the effect of pH on the kinetics were characterized . - pubmed
Substitution of Lys51 , which is located in a Walker - A motif ( GXXGXGKT , amino acids 45 - 52 ) , with Ala or His resulted in enzymes with increased KMgATP values and unable to bind Fru 6 - P. - pubmed
The pH dependence of V and the V / Ks for MgATP and Fru 6 - P suggest a mechanism in which reactants and enzyme combine irrespective of the protonation state of groups required for binding and catalysis , but only the correctly protonated enzyme - substrate complex is catalytically active . - pubmed
The application of negative upper airway pressure ( 0 to -12.5 cm H2O ) caused increases in levator palatini ( LP , p & lt ; 0.001 ) and palatoglossus ( PG , p & lt ; 0.001 ) activity , 100 msec after pressure stimulus in normal subjects . - pubmed
Permeability of beta - lactams across the outer membrane ( OM ) of M2 was lower than that across the OM of C152 . - pubmed
Mutants deficient in the 43 - kDa OMP could be selected in vitro from strain C152 in the presence of cefoxitin . - pubmed
During carbon starvation , DnaK deficiency reduced RpoS levels threefold , while DnaK excess increased RpoS levels nearly twofold . - pubmed
Important parameters involved in the activation of host - reactive T cells by nonspecific infectious agents included the presence , localization , and quantity of the specific transgene - encoded self - antigen ; in contrast , CD4 + T cells were not required . - pubmed
Following metabolic labeling of these cells with [ 14C ] galactose , the GSL were purified and the radioactivity incorporated into each individual GSL quantitated by phosphoimaging . - pubmed
Dysregulation of aquaporin-2 ( AQP2 ) , the predominant vasopressin - regulated water channel , is known to be associated with a range of congenital and acquired water balance disorders including nephrogenic diabetes insipidus and states of water retention . - pubmed
To measure the breakdown of TMH - ferrocene , iron release from the molecule was analyzed using a Ferrochem II analyzer , which uses constant potential coulometry to measure the amount of ionic iron within a biological sample . - pubmed
The TMH - ferrocenase activity is heat labile , requires a physiologic temperature , is induced by phenobarbital , and is inhibited by carbon monoxide and piperonyl butoxide but not by dicoumarol . - pubmed
The enzyme follows Michaelis - Menten kinetics and has a Km of 58.5 microM and a Vmax of 57.5 nmol Fe released / mg protein / min . - pubmed
We conclude that TMH - ferrocene is degraded by a phenobarbital - inducible cytochrome P-450 . - pubmed
They have been found to interfere at different stages of T cell activation and proliferation , and can be identified as inhibitors of nucleotide synthesis , growth factor signal transduction and differentiation . - pubmed
Cocaine - seeking behavior and Fos expression in the amygdala produced by cocaine or a cocaine self - administration environment . - pubmed
Optimal AChE mRNA localization was obtained with a digoxigenine - labeled RNA probe . - pubmed
Carbonic anhydrase inhibitors : N - cyanosulfonamides , a new class of high affinity isozyme II and IV inhibitors . - pubmed
Compounds of the types RSNHCN and RCONHCN were much less active in inhibiting all the investigated isozymes as compared to the strong inhibitors possessing the general formula RSO2NHCN . - pubmed
Susceptibility to inhibition with the N - cyanosulfonamides was generally : hCA II & gt ; bCA IV & gt;> ; hCA I. - pubmed
Spectroscopic studies on Co ( II )-substituted hCA II proved that the new inhibitors directly bind to the metal ion within the enzyme active site , similarly to the classical inhibitors of the unsubstituted sulfonamide type . - pubmed
Because many agonists stimulate ciliary motility through activation of cyclic nucleotide - dependent protein kinases , we hypothesized that ethanol stimulates ciliary motility by activating protein kinases . - pubmed
RESULTS : Ethanol markedly activated cyclic adenosine monophosphate ( cAMP )- dependent protein kinase [ protein kinase A ( PKA ) ] at biologically relevant concentrations ( 20 - 100 mM ) , with activation detectable after 15 min and persisting up to 4 hr . - pubmed
Ethanol 's PKA activation was blocked by nitric oxide synthase inhibitors , indicating a nitric oxide - dependent pathway , and was also specifically blocked by PKA inhibitors . - pubmed
Selective recognition of mannose by the human eosinophil Charcot - Leyden crystal protein ( galectin-10 ): a crystallographic study at 1.8 A resolution . - pubmed
In the absence of any known natural carbohydrate ligand for this protein , the functional role of the CLC protein ( galectin-10 ) has remained speculative . - pubmed
Here we describe structural studies on the carbohydrate binding properties of the CLC protein and report the first structure of a carbohydrate in complex with the protein . - pubmed
Interestingly , the CLC protein demonstrates no affinity for beta - galactosides and binds mannose in a manner very different from those of other related galectins that have been shown to bind lactosamine . - pubmed
The partial conservation of residues involved in carbohydrate binding led to significant changes in the topology and chemical nature of the CRD , and has implications for carbohydrate recognition by the CLC protein in vivo and its functional role in the biology of inflammation . - pubmed
Insulin - mediated vasodilation and glucose uptake are independently related to fasting serum nonesterified fatty acids in elderly men . - pubmed
Insulin - mediated glucose disposal was related to body mass index ( BMI ) ( r = -0.49 , P = 0 . - pubmed
In multivariate analysis only BMI and NEFA remained significantly related to whole - body glucose uptake . - pubmed
CONCLUSIONS : The study demonstrates that in elderly men BMI and fasting serum NEFA but not insulin - induced vasodilation are related to insulin sensitivity . - pubmed
Monoclonal antibody MIB-5 or antibodies to bromodeoxyuridine were used to identify proliferating cells . - pubmed
Determination of the disulfide bond and its possible role in tobacco acetolactate synthase . - pubmed
The mechanisms that influence the polarization of CD4 T cells specific for allogeneic MHC class II molecules in vivo are still poorly understood . - pubmed
SDS - PAGE analysis of isotope - labelled HSV - specific proteins revealed that the synthesis of beta proteins was moderately inhibited and some of the glycoproteins were synthesized with reduced molecular weights . - pubmed
From these findings , antiherpetic activities of Conmy A and B might be mainly dependent on their activities as vacuolar proton - ATPase inhibitors with intracellular translocation of glycoproteins and the inhibition of the maturation of virus glycoproteins . - pubmed
We used leptomycin B ( LMB ) , an inhibitor of nuclear export signal ( NES)-and its receptor , CRM1 / Exportin1 - mediated protein export . - pubmed
LMB efficiently inhibited vRNP export , while it did not affect the subcellular localization and export of matrix protein ( M ) 1 and nonstructural protein ( NS ) 2 . - pubmed
Second , indirect immunofluorescence assays also revealed that vRNP export is sensitive to LMB . - pubmed
Analyses using glycerol density gradients showed that a major fraction of M1 and NS2 was not complexed with the exported vRNP . - pubmed
LMB - sensitive and -insensitive NS2 . - pubmed
BACKGROUND : The crystal structure of precorrin-8x methyl mutase ( CobH ) , an enzyme of the aerobic pathway to vitamin B12 , provides evidence that the mechanism for methyl migration can plausibly be regarded as an allowed [ 1,5]-sigmatropic shift of a methyl group from C-11 to C-12 at the C ring of precorrin-8x to afford hydrogenobyrinic acid . - pubmed
The same level of DeltaPes was observed at the end of OHs and RERAs ( 21.9 + /- 5.5 versus 18.9 + /- 5.7 cm H ( 2 ) O respectively [ NS ] ) whereas the reduction in flow was as expected , higher for OH ( 57.9 + /- 10.7 versus 21.3 + /- 4.9 % ) . - pubmed
The mitogen- and stress - induced phosphorylation of CREB at Ser133 has been linked to the transcription of several immediate early genes , including c - fos , junB , and egr1 . - pubmed
The knockout of both MSK1 and MSK2 resulted in a 50 % reduction in c - fos and junB gene transcription in response to anisomycin or UV - C radiation but only a small reduction in response to tetradecanoyl phorbol acetate or epidermal growth factor in fibroblasts . - pubmed
Chromatographic results suggest that BNP interacts approximately the same with both the C- and N - terminal amino acid of poly SULL , while the preferential site of interaction of this analyte with the monomeric form of SULL ( mono SULL ) is at the C - terminal amino acid . - pubmed
This effect was antagonized by WIN55,212 - 2 and abolished by N - ethylmaleimide , consistent with the involvement of cannabinoid CB ( 1 ) receptors through the activation of G ( i / o ) proteins . - pubmed
Following sequences were acquired : T 2 -w TSE and T 1 -w FLASH , after administration of gadolinium , 6 VIBE sequences with iPAT ( TR / TE / matrix / partition thickness / time of acquisition : 6.2 ms/ 3.2 ms/256 x 192/4 mm/13 s ) , as well as T 1 -weighted FLASH with fat saturation . - pubmed
Myostatin is a potent inhibitor of muscle growth . - pubmed
METHODS : 15 EAs were observed by means of HE staining , immunohistochemical staining ( ABC method ) and in part by electron microscopy . - pubmed
on the pharmacokinetics and brain distribution of the adenosine A1 receptor partial agonist 2 '- deoxy - N6 - cyclopentyladenosine ( 2'dCPA ) , and ( 2 ) the effect of 2'dCPA ( 20 mg / kg i.v . ) - pubmed
VEGF concentrations in the cell culture supernatant were determined by enzyme - linked immunosorbent assay ( ELISA ) , and cell proliferation was determined by MTT assay and colony - forming assay in vitro . - pubmed
This is supplemented with HBO given in 90 - minute sessions at approximately 2.5 ATM of pure oxygen for 20 sessions prior to surgical intervention and followed by 10 additional postoperative dives . - pubmed
A prior comparison of 750 mg flutamide daily to 500 mg daily with an LHRH analog or orchiectomy showed no difference in effect on prostate specific antigen ( PSA ) . - pubmed
Serum samples at the initiation of therapy and at the 1- and 3 - month time point were assessed for PSA , testosterone , liver function tests , hematology , and renal function . - pubmed
Testosterone and PSA levels show a dose - dependent response to flutamide monotherapy . - pubmed
Flutamide at 500 mg did not result in significant changes in testosterone , PSA , prostate volume , or androgen deficiency symptoms compared to 750 mg daily after 3 months . - pubmed
Deletion and aberrant CpG island methylation of Caspase 8 gene in medulloblastoma . - pubmed
We determined the frequency of aberrant CpG island methylation for Caspase 8 ( CASP8 ) in a group of 24 medulloblastomas arising in 8 adult and 16 pediatric patients . - pubmed
Mucosa - free circular muscle strips were precontracted with methacholine in the presence of guanethidine and exposed to electrical field stimulation ( EFS ) and exogenous NO , ATP , VIP and PACAP . - pubmed
NO ( 10(-6)-10(-4 ) m)-induced concentration - dependent relaxations that were inhibited by the soluble guanylyl cyclase inhibitor 1H -[ 1,2,4 ]- oxadiazolo -[ 4,3 - a ]- quinoxalin-1 - one ( ODQ ; 10(-5 ) m ) and the small conductance Ca ( 2 +)- activated K (+)- channel blocker apamin ( APA ; 3 x 10(-8 ) m ) . - pubmed
Relaxations elicited by exogenous ATP ( 10(-4)-10(-3 ) m ) were inhibited by the P2Y purinoceptor antagonist reactive blue 2 ( RB2 ; 3 x 10(-4 ) m ) , but not by APA and ODQ . - pubmed
The inhibitory responses evoked by 10(-7 ) m VIP and 3 x 10(-8 ) m PACAP were decreased by the selective PAC ( 1 ) receptor antagonist PACAP(6 - 38 ) ( 3 x 10(-6 ) m ) and APA . - pubmed
The VPAC ( 2 ) receptor antagonist PG99 - 465 ( 3 x 10(-7 ) m ) reduced relaxations caused by VIP , but not those by PACAP , while the VPAC ( 1 ) receptor antagonist PG97 - 269 ( 3 x 10(-7 ) m ) had no influence . - pubmed
EFS - induced relaxations were inhibited by the NO - synthase inhibitor N ( omega )- nitro - l - arginine methyl ester ( 3 x 10(-4 ) m ) , ODQ and APA , but not by RB2 , PG97 - 269 , PG99 - 465 and PACAP ( 6 - 38 ) . - pubmed
Here , we compared two methods of GAD65Ab epitope analysis : GAD65 / 67 fusion proteins and competition assays using GAD65 - specific recombinant fraction antigen binding ( rFab ) . - pubmed
Fusion proteins allow the definition of large epitope regions ; however , some conformational GAD65Ab epitopes , especially those residing in the middle region , are destroyed or distorted in the fusion proteins . - pubmed
This treatment did not increase the functional affinity for acetylcholine at type II nAChRs . - pubmed
A single injection of nicotine also produced a significant increase in type III nAChR response . - pubmed
In addition , type III and type II , but not type IA nAChRs , are desensitized by in vitro exposure to nicotine at concentrations found in the venous blood of cigarette smokers . - pubmed
Bupropion at 1 muM produced 56 , 15 , and 0 % inhibition of type III , type II , and type IA nAChR responses , respectively , in the slices . - pubmed
Our results suggest that in vivo - nicotine - induced nAChR up - regulation observed in neurons of intact brain tissue is a physiologically relevant phenomenon and that early up - regulation of type III and type II nAChRs could be an important biological signal in nicotine addiction . - pubmed
Abeta and its precursor protein ( APP ) interact with metal ions such as zinc , copper and iron . - pubmed
Insulin - mediated signal transduction is negatively regulated by the phosphotyrosine phosphatase , PTP1B , and numerous studies have demonstrated that organo - vanadium compounds , which are nonselective phosphotyrosine phosphatase inhibitors , have insulin - mimetic properties . - pubmed
However , whether or not vanadium compounds can prevent the transition from insulin resistance to overt diabetes is unknown . - pubmed
Roxithromycin inhibits angiogenesis of human hepatoma cells in vivo by suppressing VEGF production . - pubmed
Inter - laboratory differences in sirolimus results from six sirolimus testing centers using HPLC tandem mass spectrometry ( LC / MS / MS ) . - pubmed
Leflunomide has been shown to cause cell specific inhibition of inducible nitric oxide synthase ( iNOS ) activation in animal models . - pubmed
When gallic acid purified from rose flowers was used to treat the 9 - month - old male SAM , it not only reinstated the activities of CAT and GPx but also significantly reduced the amount of malondialdehyde ( MDA ) in the liver , brain and kidney . - pubmed
OBJECTIVES : MRI T2 hypointensity in multiple sclerosis ( MS ) gray matter , suggesting iron deposition , is associated with physical disability , disease course , lesion load , and brain atrophy . - pubmed
HPLC determination of enoxacin , ciprofloxacin , norfloxacin and ofloxacin with photoinduced fluorimetric ( PIF ) detection and multiemission scanning : application to urine and serum . - pubmed
According to the criterium of Clayton , using 0.05 as false positive and false negative error assurance probabilities , detection limits of 7.3 , 6.0 , 6.3 and 14.5 ng / mL , for ENO , NOR , CIPRO and OFLO , respectively , have been found . - pubmed
In the present work , we diagonalize the Hessian matrix of the polarizability with respect to the vibrational nontotally symmetric normal coordinates , to calculate the nontotally symmetric distortions that produce the maximum breakdown of the MPP in a series of twenty polycyclic aromatic hydrocarbons . - pubmed
Arbuscular mycorrhizal fungal ( AMF ) spore communities were surveyed in a long - term field fertilization experiment in Switzerland , where different amounts of phosphorus ( P ) were applied to soil . - pubmed
Well - resolved one - dimensional and two - dimensional NMR spectra of cytochrome P450 BM-3 reveal shifts upon binding of its substrate , N - palmitoylglycine . - pubmed
Plant signal molecules like salicylic acid , methyl jasmonate , ethylene and spermine , known to be involved in plant defense were not able to induce the investigated genes , therefore , an unknown signalling mechanism is expected to operate in BR . - pubmed
CONCLUSIONS : Antioxidant vitamin supplementation during IFN - alpha-2b and ribavirin therapy prevented a decrease in EPA of mononuclear cell phospholipids . - pubmed
If a further decrease in the ratio of arachidonic acid to EPA can be achieved by using oral EPA supplementation , the efficacy of IFN - alpha-2b and ribavirin therapy may be improved . - pubmed
To investigate mechanisms of the basal activity of AHA angiotensin II - sensitive neurons , we examined the effect of N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalenesulfonamide hydrochloride ( W7 ) , a calmodulin inhibitor , applied onto AHA neurons on the basal activity and the stimulus - evoked activation of these neurons . - pubmed
Microinjections of carbachol into the LSV and corticotropin - releasing factor into the MeA , and intracerebroventricular injection of hypertonic saline , activated AHA angiotensin II - sensitive neurons . - pubmed
These three kinds of injection - induced activations of AHA neurons were abolished by pressure application of W7 onto the same neurons , while the calmodulin inhibitor did not affect the increase in firing of AHA neurons induced by pressure application of angiotensin II onto the same neurons . - pubmed
The pressure application of W7 did not affect the basal activity of AHA angiotensin II - sensitive neurons , whereas the angiotensin AT1 receptor antagonist losartan similarly applied inhibited it . - pubmed
Compared to the control side , the tetracycline labeling lines were wider ; the increase of the expression level of BMP-2 appeared earlier , greater and lasted longer in the tension area of the experimental side . - pubmed
We conclude that the primary cause of increased estrogen levels in acute illness is increased aromatase P450 gene expression , resulting in enhanced aromatization of androgens to estrogens , a previously undescribed endocrine response to acute illness . - pubmed
Binding of LT - I to cells , when GM1 was blocked by the cholera toxin B subunit , was characterized by an increased number of LT - I receptors with respect to undifferentiated control cells . - pubmed
Moreover , both types of differentiated cells accumulated higher amounts of cyclic AMP in response to LT - I than undifferentiated cells . - pubmed
Helix pomatia lectin inhibited the binding of LT - I to cells and the subsequent production of cyclic AMP . - pubmed
Associations of body size at birth with late - life cortisol concentrations and glucose tolerance are modified by haplotypes of the glucocorticoid receptor gene . - pubmed
Moreover , this haplotype modified the association of length at birth with adult phenotypes ; in carriers , short length at birth was associated with increased fasting plasma cortisol , cortisol / corticosteroid - binding globulin ratio , impaired glucose tolerance or diabetes [ 1 cm decrease corresponded to 1.36 - fold odds ratio ; 95 % confidence interval ( CI ) , 1.09 - 1.70 ; P = 0.007 ] , and higher 120 - min glucose ( 5.8 % ; 95 % CI , 2.5 - 9.1 % ; P = 0.0007 ) , but no association was seen in noncarriers ( P for interaction was 0.06 , 0.01 , 0.02 , and 0.01 , respectively ) . - pubmed
CONCLUSIONS : A common GR haplotype may contribute to and modify the association of short length at birth with adult glucose tolerance and HPAA function by a mechanism that affects regulation of GR expression . - pubmed
HSE produced a concentration - dependent vasodilation ( IC50 = 10.8 + /- 4.0 microg / mL ) in arterial rings pre - contracted with phenylephrine , which was completely abolished in endothelium - denuded vessels . - pubmed
Endothelium - dependent vasodilation induced by HSE was strongly reduced by L - NAME ( 100 microM ) , a nitric oxide ( NO ) synthase inhibitor , but neither by atropine , a muscarinic receptor antagonist ( 1 microM ) , nor by indomethacin ( 10 microM ) , a cyclooxygenase inhibitor . - pubmed
In rings pre - contracted with 80 mM KCl , the vasodilator effect of HSE was shifted to the right and was completely abolished in the presence of L - NAME ( 100 microM ) . - pubmed
In addition , BaCl2 ( 1 mM ) dramatically reduced the vasodilation induced by HSE . - pubmed
The goal of this study was to determine if prenatal exposure to cocaine was associated with an increase in the number of excitatory synapses on dendritic spines in layer II / III of the prelimbic cortex . - pubmed
This response was suppressed by approximately 50 % when cells were pretreated with the B1 receptor antagonist des - Arg9 -[ Leu8 ]- bradykinin while treatment with des - Arg9 - bradykinin , the B1 receptor agonist , caused a concentration - dependent increase in hCXCL5 and hCXCL6 mRNA expression . - pubmed
Do insulin ( or IGF-1 ) and glucagon act oppositely in controlling eye growth , as they do in metabolic pathways and in control of retinal neurogenesis ? - pubmed
In eyes without lenses , both insulin and IGF-1 speeded , and glucagon slowed , ocular elongation , but glucagon and insulin each increased the rate of thickening of the crystalline lens . - pubmed
When injected together , insulin blocked choroidal thickening by glucagon , at a dose that did not , by itself , thin the choroid . - pubmed
CONCLUSIONS : Glucagon and insulin ( or IGF-1 ) cause generally opposite modulations of eye growth , with glucagon mostly increasing choroidal thickness and insulin mostly increasing ocular elongation . - pubmed
Using cells expressing recombinant alpha3beta4 nAChRs , these molecules inhibited calcium accumulation ( a more direct assay to establish nAChR activity ) , with IC(50 ) values ranging from 0.7 to 38.2 microM. - pubmed
IGF-1 treatment increased phospho - Akt fourfold at 30 min , cyclins D1 and E by 6 h , and decreased p27 ( KIP1 ) and p57 ( KIP2 ) expression . - pubmed
The biological effects of FGFs are mediated through the activation of the four transmembrane phosphotyrosine kinase receptors ( FGFRs ) in the presence of heparin sulfate proteoglycans ( HSPGs ) and therefore require the release of FGFs into the extracellular space . - pubmed
To understand the molecular events in the FGF-1 releasing pathway , we have studied the interactions of S100A13 with C2A by ( 1 ) H -( 15 ) N HSQC titration and 3D - filtered NOESY experiments . - pubmed
The most promising application is the selective interaction of covalently fixed boronic acid residues with glucose , which renders itself not only for sensing , but eventually also for delivery of drugs such as insulin . - pubmed
Those taking TDF and a PI / r had a greater median decline in eGFR than those taking TDF and a non - NRTI at 6 months ( P = 0.01 ) , with trends at 12 ( P = 0.08 ) and 24 months ( P = 0.08 ) . - pubmed
Our results support the use of TDF as a component of the initial antiretroviral regimen , and suggest that the eGFR should be monitored more closely when TDF is used with a PI / r. - pubmed
SUCROSE SYNTHASE ( SUS : EC 2.4.1.13 ) , a key enzyme in plant sucrose catabolism , is uniquely able to mobilize sucrose into multiple pathways involved in metabolic , structural , and storage functions . - pubmed
A constructed atu2422 mutant was more virulent than the wild type bacterium ; moreover , transgenic plants with a low level of Pro exhibited less severe tumor symptoms than did their wild - type parents , revealing a crucial role for Venus flytrap GABA - receptor and relative abundance of GABA and Pro in host - pathogen interaction . - pubmed
Nicotinic acetylcholine and adenosine type 1 and 2A densities were significantly down - regulated in the putamen of HE brains . - pubmed
2MeSADP reduced cell proliferation and increased Caspase-3 activity ; both these effects could be fully reversed by the P2Y ( 13 ) receptor antagonist MRS2211 . - pubmed
These results indicate that P2Y ( 13 ) is a proapoptotic receptor in beta - cells as the P2Y ( 13 ) receptor antagonist MRS2211 is able to protect the cells from ADP induced apoptosis . - pubmed
We examined the effects of interleukin-1 beta ( IL-1beta ) , tumor necrosis factor alpha ( TNF - alpha ) , lipopolysaccharide ( LPS ) , lipoteichoic acid ( LTA ) , zymosan ( ZYM ) , dexamethasone ( DEX ) , cyclosporine , and tacrolimus on transendothelial electrical resistance ( TEER ) ; endothelial tight junctions ; filamentous actin ; and permeability to deoxycholate AMB ( DAMB ) , liposomal AMB ( LAMB ) , and fluconazole . - pubmed
TNF - alpha and LPS increased the permeability to AMB by 8.2 to 14.5 % compared to that for the controls ( 1.1 to 2.4 % ) ( P & lt ; or = 0.04 ) . - pubmed
In conclusion , IL-1beta , ZYM , and LTA increased the permeability of the BBB to small ions but not to AMB , whereas TNF - alpha and LPS , which disrupted the endothelial layer integrity , increased the permeability to AMB . - pubmed
Mutation analysis of mitochondrial 12S rRNA gene in Polish patients with non - syndromic and aminoglycoside - induced hearing loss . - pubmed
In the present study , we have performed mutational screening of entire 12S rRNA gene in 250 unrelated patients with non - syndromic and aminoglycoside - induced hearing loss . - pubmed
Moreover , two 12S rRNA sequence variants : m.988 G>A and m.1453 A>G , localized at conserved sites and affected RNA secondary structure , may be new candidates for non - syndromic and aminoglycoside - induced hearing loss associated mutations . - pubmed
AIM : Because of its zinc - free formulation insulin glulisine ( GLU ) might have a faster onset of action than other short - acting analogues . - pubmed
We compared the pharmacokinetics and pharmacodynamics of GLU with those of insulin aspart ( ASP ) . - pubmed
RESULTS : GLU showed a significantly higher early metabolic effect ( area under the glucose infusion rate ( GIR ) curve in the first 30 min AUC - GIR ( 0-)30 min 30.3 + - 26.4 vs. 16.2 + - 18.4 mg / kg , P = 0.0421 ) than ASP , an earlier onset of action ( time to 10 % of GIR ( max ) ( GIR ( max)-t ( 10 % ) ) 9 vs. 17 min , P = 0.0146 ) and a faster absorption ( shorter times to 10 % and 20 % of INS ( max , ) P = 0.0005 each ) . - pubmed
Finally , HbA1c testing might be biased due to the interference from several hemoglobin variants , is characterized by a higher imprecision than blood glucose measurement , and is more expensive . - pubmed
We demonstrate that 2G12 is produced with complex N - glycans at great uniformity in the wt as well as in the glycosylation mutant , carrying a single dominant glycosylation species , GnGnXF and GnGn , respectively . - pubmed
OBJECTIVE : The substitution of selenium activates the selenium - dependent enzyme glutathione peroxidase , which is important for scavenging free radicals . - pubmed
Paclitaxel ameliorates fibrosis in hepatic stellate cells via inhibition of TGF - beta / Smad activity . - pubmed
RESULTS : The paclitaxel treatment markedly suppressed Smad2 / 3 phosphorylation . - pubmed
Self - assembly of the recombinant hydrophobin was followed kinetically using a thioflavin T fluorescence binding assay , and contact angle measurements of purified recombinant hydrophobin protein ( mHyd2 ) films on a variety of substrata demonstrated its surface modification ability , which remained stable for at least 4 months . - pubmed
The rest of the metal stent was purged with NaCl 0.9 % . - pubmed
This enzyme is likely to be a cysteine protease ; it was found active in media containing EDTAK2 and dithiothreitol , it maintained considerable activity at temperatures 14 degrees C to 33 degrees C and pH 6.50 to 8.50 with optimum k(cat)/Km and/or k(cat ) values at pH 7.00 and 25 degrees C. The activity of protease - B-17N-1 was strongly affected by the specific irreversible inhibitor of cysteine proteases E-64 , while it remained unaffected by the 3,4 - dichloro - isocoumarine , an irreversible inhibitor specific for serine proteases . - pubmed
Protease - B-17N-1 retained full activity at 25 degrees C after 30 min incubation at 8 degrees C or at 33 degrees C ; moreover , it was found to be stable and active in the polar organic solvents DMSO and acetonitrile . - pubmed
The enzyme hydrolyzed the substrate Cbz - FR - pNA via Michaelis - Menten kinetics , while it showed insignificant activity for the substrate Suc - AAA - pNA . - pubmed
In addition , interesting results were obtained from the effect of different metallic ions and polar organic solvents on the Michaelis - Menten parameters of protease - B-17N-1 , showing that it performs catalysis via a ( Cys )- S (-)/( His )- Im (+) H ion - pair , as well as its industrial and biotechnological potential , respectively . - pubmed
The phagocytic uptake of apoptotic / necrotic cells involves a plethora of molecules , including immunoglobulins , lectins , components of the complement system ( all of which act as opsonins ) , as well as the phospholipid phosphatidylserine ( PS ) and the endoplasmic reticulum chaperone calreticulin ( CRT ) , both of which can be exposed on the surface of dying cells . - pubmed
Recently , this view has been challenged by the observations that , similar to PS , CRT acts as a facultative recognition ligand on apoptotic cells , and that cytotoxic agents such as anthracyclines induce the exposure of CRT on the surface of dying tumor cells , thereby generating an engulfment signal that stimulates the uptake of apoptotic corpses and the presentation of the corresponding antigens by dendritic cells . - pubmed
Other neural areas that may interact with this putative circuit include amygdala , ventral hypothalamus , periaqueductal gray ( PAG ) , and deep tectum , as well as ascending dopamine systems which participate in all types of seeking urges . - pubmed
In the MD and TD groups , FSH injections were begun on the Day 8 of progestagen treatment . - pubmed
Database water composition is dominated by sodium bicarbonate and sodium chloride type waters with total dissolved solids concentrations of 150 to 39,260 mg / L. Constituents commonly exceeding standards for drinking , livestock , and irrigation water applications were total dissolved solids ( TDS ) , sodium adsorption ratio ( SAR ) , temperature , iron , and fluoride . - pubmed
Targeting IL5 with mepolizumab seems promising in sparing steroids , but relapses often follow its withdrawal . - pubmed
The low - frequency ( LF)/high - frequency ( HF ) power ratio , an index of cardiac sympathovagal balance , was significantly lower with efonidipine and cilnidipine than with amlodipine , while the HF / total power ratio , an index of cardiac vagal activity , revealed the opposite results . - pubmed
There was no significant correlation between the LF / HF ratio and plasma norepinephrine levels . - pubmed
PURPOSE : The aim of the present study was to investigate plasma concentrations of Gas6 and its soluble tyrosine kinase receptor sAxl in Systemic lupus erythematosus ( SLE ) and Behcets disease ( BD ) patients and to correlate those levels with clinical and laboratory manifestations of the diseases . - pubmed
The InSb working standard was also measured at the National Physical Laboratory ( NPL ) of the United Kingdom in 1999 . - pubmed
The less than 2 % spectral power responsivity disagreements obtained on the InSb working standard ( both from the 1999 NIST and NPL comparison and also against the pyroelectric standard ) validate the three independently realized power responsivity scales and verify the long - term stability of the InSb working standard . - pubmed
We further show that UHRF1 association with H3K9 methylation is insensitive to adjacent H3 S10 phosphorylation -- a known mitotic ' phospho - methyl switch ' . - pubmed
Accelerated mitochondrial aging induced by nucleoside reverse transcriptase inhibitors ( NRTIs ) has been implicated as a part of this phenomenon . - pubmed
Recent studies suggest that RAS blockers may reduce inflammation by increasing endogenous concentrations of the anti - inflammatory heptapeptide angiotensin -( 1 - 7 ) [ Ang -( 1 - 7 ) ] . - pubmed
Treatment with U-0126 , a MEK inhibitor , demonstrates that this radiation - induced inflammation in astrocytes is mediated through the MAP kinase pathway . - pubmed
Ang -( 1 - 7 ) inhibits radiation - induced inflammation , increases in PKCalpha , and MAP kinase pathway activation ( phosphorylation of MEK and ERK ) . - pubmed
Additionally Ang -( 1 - 7 ) treatment leads to an increase in dual specificity phosphatase 1 ( DUSP1 ) . - pubmed
Furthermore , treatment with sodium vanadate ( Na3VO4 ) , a phosphatase inhibitor , blocks Ang -( 1 - 7 ) inhibition of radiation - induced inflammation and MAP kinase activation , suggesting that Ang -( 1 - 7 ) alters phosphatase activity to inhibit radiation - induced inflammation . - pubmed
There are many proposed pharmacological treatments including : beta - blockers , angiotensin - converting enzyme inhibitor ( ACE inhibitors ) , angiotensin - receptor blocker ( ARB ) , statins , macrolides and , doxycycline , an inhibitor of the MMP . - pubmed
The structural and magnetic properties of nanostructured Co - rich transition - metal alloys , Co(100 - x)TMx ( TM = Hf , Zr and 10 & lt;= x & lt;= 18 ) , were investigated . - pubmed
PBDE exposures for 96 h have caused chitobiase and chitinolytic enzymes inhibition . - pubmed
BACKGROUND : To investigate the antioxidant value and anticancer functions of mitragynine ( MTG ) and its silane - reduced analogues ( SRM ) in vitro . - pubmed
MATERIALS AND METHODS : MTG and SRM was analyzed for their reducing power ability , ABTS radical inhibition and 1,1 - diphenyl-2 - picryl hydrazylfree radicals scavenging activities . - pubmed
RESULTS : SRM and MTG demonstrate moderate antioxidant value with ABTS assay ( Trolox equivalent antioxidant capacity ( TEAC ): 2.25 + -0.02 mmol trolox / mmol and 1.96 + -0.04 mmol trolox / mmol respectively ) and DPPH ( IC50=3.75 + -0.04 mg / mL and IC50=2.28 + -0.02 mg / mL respectively ) . - pubmed
Three variables ( C - reactive protein , total cholesterol , and instability of total sleep time ) contributed to a combined moderator of faster decrease in BMI with IRRI treatment . - pubmed
To assess thyroid function , serum thyroid stimulating hormone ( TSH ) , free thyroxine ( FT4 ) , free triodothyronine ( FT3 ) and TPOAb / thyroglobulin antibody ( TGAb ) levels were determined using chemiluminescent immunoassays every 3 mo . - pubmed
FLNB transcript analysis of the c.2055 G & gt ; C variant revealed insertion of 27 bp intronic sequence between exon 13 and 14 which gives rise to in - frame deletion of glutamine 685 and insertion of ten novel amino acid residues ( p. Gln685delins10 ) . - pubmed
Of untreated SCB about 63 % of the lignin compounds were assigned as p - coumarates and ferulates , analysed by pyrolysis - GC / MS as 4 - vinyl phenol and 4 - vinyl guaiacol , and designated as non - core lignin ( NCL ) compounds . - pubmed
The first sugar of the repeat units is essential for the Wzy polymerase activity and elongation of the O - antigen lipopolysaccharide . - pubmed
In the Wzx / Wzy - dependent assembled pathway , the assembled O - antigen repeat units are translocated from the cytosolic to the periplasmic face of the inner membrane by a Wzx translocase and then polymerized by the integral membrane protein Wzy to form a glycan chain . - pubmed
OBJECTIVE : To prepare the slow - release complex with rifampicin ( RFP)-polylactic - co - glycolic acid ( PLGA)-calcium phosphate cement ( CPC ) ( RFP - PLGA - CPC complex ) , and to study its physical and chemical properties and drug release properties in vitro . - pubmed
: METHODS : The emulsification - solvent evaporation method was adopted to prepare rifampicin polylactic acid - glycolic acid ( RFP - PLGA ) slow - release microspheres , which were divided into 3 groups : a calcium phosphate bone cement group ( CPC group ) , a CPC embedded with RFP group ( RFP - CPC group ) , and a PLGA slow - release microspheres carrying RFP and the self - curing CPC group ( RFP- PLGA - CPC complex group ) . - pubmed
: RESULTS : The CPC group showed the shortest solidification time , while the RFP - PLGA - CPC complex group had the longest one . - pubmed
There was statistical difference in the porosity between the CPC group and the RFP - CPC group ( P<0.05 ) ; Compared to the RFP - PLGA - CPC complex group , the porosity in the CPC group and the RFP - CPC group were significantly changed ( both P<0.01 ) . - pubmed
There was significant difference in the compressive strength between the RFP- PLGA - CPC complex group and the CPC group ( P<0.01 ) , while there was significant difference in the compressive strength between the RFP - CPC group and the CPC group ( 3 days : P<0.05 ; 30 and 60 days : P<0.01 ) . - pubmed
The pores of section in the RFP - PLGA - CPC complex group increased obviously , and PLGA microspheres gradually disappeared until the 60th day when there were only empty cavities left . - pubmed
The RFP - PLGA - CPC complex group had no obvious drugs sudden release , and the cumulative drug release rate was nearly 95 % in the 60 days . - pubmed
To isolate MAP , the double incubation method was used for decontaminating the tissues and Middlebrook 7H9 broth - based culture associated with OADC ( oleic acid , albumin , dextrose , and catalase ) supplement with / without mycobactin J were used . - pubmed
Phase 2 had a greater proportion of vancomycin courses with therapeutic initial trough concentrations ( 27.5 % vs 46.1 % ; p = 0.002 ) , higher initial trough concentrations ( 10.9 mcg / mL vs 16.4 mcg / mL ; p & lt ; 0.001 ) , and optimized initial vancomycin dosing ( 13.5 mg / kg vs 16.2 mg / kg ; p & lt ; 0.001 ) . - pubmed
Severe hypertriglyceridemia is defined at a plasma triglyceride ( TG ) concentration of & gt;885 mg / dl and may result - in particular when clinical symptoms appear before the age of 40 - from " large variant " mutations in genes which influence the function of the lipoprotein lipase ( LPL ) . - pubmed
Omega-3 - Fatty acids and fibrates can be used to lower plasma TG levels . - pubmed
The structures of K11 and K83 CPS produced by isolates LUH5545 and LUH5538 , which carry related KL11a and KL83 gene clusters , respectively , were established by sugar analysis and one- and two - dimensional 1H and 13C NMR spectroscopy . - pubmed
Both CPS contain l - rhamnose ( l - Rha ) and 6 - deoxy - l - talose ( l-6dTal ) , and both KL gene clusters include genes for dTDP - l - Rhap synthesis and a tle ( talose epimerase ) gene encoding an epimerase that converts dTDP - l - Rhap to dTDP - l-6dTalp . - pubmed
The K11 and K83 repeat units are the same pentasaccharide , consisting of d - glucose , l - Rha , l-6dTal , and N - acetyl - d - glucosamine , except that l-6dTal is 2 - O - acetylated in K83 . - pubmed
However , the K units are linked differently , with l - Rha in the main chain in K11 , but as a side - branch in K83 . - pubmed
Combined assessment of DYRK1A , BDNF and homocysteine levels as diagnostic marker for Alzheimer ' s disease . - pubmed
Receiver - operating characteristic curves and logistic regression analyses showed that combined assessment of DYRK1A , BDNF and homocysteine has a sensitivity of 0.952 , a specificity of 0.889 and an accuracy of 0.933 in testing for AD . - pubmed
RESULTS : The ratio of partial pressure of arterial oxygen to the fraction of inspired oxygen only dropped markedly from before CPB in the control group ( P & lt ; .001 ) . - pubmed
Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS - Mediated PI3K / AKT Signalling . - pubmed
Western blot analysis showed that AKT activity was significantly downregulated after DSN treatment , and that inhibition / ectopic expression of AKT enhanced / abolished DSN - induced apoptosis but not migration . - pubmed
Furthermore , we confirmed the relationship between ROS and the PI3K / AKT pathway and found that DSN induced apoptosis by regulating ROS - mediated PI3K / AKT signaling . - pubmed
Taken together , these findings indicate that DSN induces GBC apoptosis through inhibiting ROS - mediated PI3K / AKT signalling . - pubmed
Efficacy and safety of proprotein convertase subtilisin - kexin type 9 ( PCSK9 ) inhibitors , alirocumab and evolocumab , a post - commercialization study . - pubmed
These data suggest that 19H3 is the preferable antibody to use to detect 4 - 1BBL in the presence of its receptor . - pubmed
Transmission electron microscopy ( TEM ) revealed that PLGA NPs and GNPs were spherically shaped , but GNPs had smooth surface while PLGA NPs had a capsular morphology with a wrinkled surface and large voids . - pubmed
The results of TEM and the redshift spectrum demonstrated that GNPs successfully bound to the surface of the PLGA NPs . - pubmed
The sugar chain - binding properties of the scFv was evaluated by array - type Sugar Chip immobilized with a library of synthetic glycosaminoglycan disaccharide structures . - pubmed
Highly sulphated disaccharide structures were found to have high affinity to scFv . - pubmed
Aimed to unravel their conformational properties we have performed a series of molecular dynamics simulations of Chitosan / Chitlac decamers , exploring different degrees of substitution ( DS ) of lactitol side chains . - pubmed
[ Sar1 , Ile8]AngII interacts with both the ' core ' binding domain , where the small - molecule ligands of AT1R and AT2R bind , and the ' extended ' binding domain , which is equivalent to the allosteric modulator binding site of muscarinic acetylcholine receptor . - pubmed
Stereoselective Double Reduction of 3 - Methyl-2 - cyclohexenone , by Use of Palladium and Platinum Nanoparticles , in Tandem with Alcohol Dehydrogenase . - pubmed
The combination of metal nanoparticles ( Pd or Pt NPs ) with NAD - dependent thermostable alcohol dehydrogenase ( TADH ) resulted in the one - flask catalytic double reduction of 3 - methyl-2 - cyclohexenone to 3 -( 1S,3S )- methylcyclohexanol . - pubmed
Resuming , the chemocatalysts were very sensitive to deactivation , which was not related to the amount of enzyme used , while the inhibition of the biocatalyst can be strongly reduced by minimizing the NPs / TADH ratio used to catalyze the reaction . - pubmed
The as - prepared NiFe / GO hybrid was characterized by transmission electron microscopy ( TEM ) and X - ray diffraction ( XRD ) . - pubmed
AIM : This randomized controlled cross - over - design - study investigated short - term effects on oral malodour of a zinc - lactate - containing mouthwash . - pubmed
It has already been shown that CK isoforms play an important role in energy homeostasis by catalyzing a reversible transfer of the phosphate of ATP to creatine to yield ADP and creatine phosphate ( CP ) ( creatine / CP circuit ) . - pubmed
Histone deacetylase 6 ( HDAC6 ) primarily catalyzes the removal of acetyl group from the side chain of acetylated lysine residues in cytoplasmic proteins such as alpha - tubulin and HSP90 . - pubmed
We herein report our new generation of multifunctional HDAC6 degraders by tethering selective HDAC6 inhibitor Nexturastat A with CRBN ligand that can synergize with HDAC6 degradation for the antiproliferation of multiple myeloma ( MM ) . - pubmed
Phosphorylated claudin-16 interacts with Trpv5 and regulates transcellular calcium transport in the kidney . - pubmed
Together , these results suggest a pathogenic mechanism for FHHNC involving transcellular Ca2 + pathway in the DCT and identify a molecular component in renal Ca2 + homeostasis under direct regulation of PTH . - pubmed
PbSe materials are typically characterized with impact ionization which effectively induces carrier multiplication ( CM ) in the quasi Type - II PbCdSe composite , exhibiting reasonably high PCE of 6.14 % with current output of 19.2 mA cm-2 for the optimal PbCdSe film . - pubmed
And lipopolysaccharide - induced neuroinflammatory state resulted in significant depression - like behaviors , including reduced locomotor activity in the open field test , reduced saccharin preference , added immobility time in tail suspension test and forced swimming test , decreased comb time in the splash test , and increased latency to food in the novelty suppressed feeding test time , and reduced the levels of neurotrophic factors and synaptic proteins , and decreased Nissl bodies . - pubmed
Aloin Preconditioning Attenuates Hepatic Ischemia / Reperfusion Injury via Inhibiting TLR4 / MyD88 / NF - kappaB Signal Pathway In Vivo and In Vitro . - pubmed
Results : We found that 20 mg / kg was the optimum concentration of aloin for mitigating I / R - induced liver tissue damage , characterized by decreased serum alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) . - pubmed
Moreover , aloin inhibited hepatocyte apoptosis and inflammatory response that was caused by the upregulated expression of Bcl-2 , the downregulated expression of cleaved caspase3(C - caspase3 ) , Bax , Toll - like receptor 4 ( TLR4 ) , FADD , MyD88 , TRAF6 , phosphorylated IKKalpha / beta ( p - IKKalpha / beta ) , and phosphorylated nuclear factor kappaB p65 ( p - NF - kappaB p65 ) . - pubmed
Oxygen Saturation ( SaO2 % ) was assessed at baseline and after exercise , while RPE and HR were measured each 10 min during the trial . - pubmed
The Area Under the Curve ( AUC ) of RPE was lower in hypoxia + CHO compared to hypoxia . - pubmed
The AUC of the HR / RPE ratio in the hypoxia + CHO group was higher in relation to hypoxia . - pubmed
CONCLUSIONS : Our results indicate that CHO supplementation does not change RPE induced by 60 min of exercise at 50 % VO2peak in hypoxia equivalent to 4200 m at the different times analyzed . - pubmed
However , in hypoxia + CHO the ( AUC)-60 min of total RPE decreased during exercise , while the heart rate / RPE ratio improved , indicating lower RPE in the hypoxic environment . - pubmed
Cognitive performance as well as biochemical markers [ ( interleukin-16 ( IL-16 ) and tumor necrosis factor- ( TNF- ) ] levels , reactive oxygen species ( ROS ) production , synaptophysin and glutamate levels ) have been evaluated at the end of um - PEA treatment . - pubmed
However , it remains poorly defined that whether icariin could inhibit ER stress and NF - kappaB mediated apoptosis in asthma and further influence the central neural system . - pubmed
We found that icariin could remarkably improve pulmonary function and reduce inflammatory cells in the lung , levels of inflammatory cytokines , and ER stress related proteins as well as NF - kappaB were prominently suppressed by icariin . - pubmed
Carbon export from leaves is controlled via ubiquitination and phosphorylation of sucrose transporter SUC2 . - pubmed
In most plants , photosynthetically produced sucrose is the predominant carbon and energy source , whose transport from leaves to carbon sink organs depends on sucrose transporters . - pubmed
An in vivo assay based on a fluorescent sucrose analog confirmed that SUC2 phosphorylation by WAKL8 can increase transport activity . - pubmed
CUDC-101 , a poorly water soluble multi - targeted anti - cancer drug that simultaneously inhibits the receptor tyrosine kinases ( RTKs ) EGFR and HER2 , as well as histone deacetylase ( HDAC ) , was encapsulated in polymeric Molecular Envelope Technology ( MET ) NPs . - pubmed
Polymer MET nanoparticles coated with hyaluronidase enabled the subcutaneous delivery of a hydro - phobic drug with favourable therapeutic outcomes . - pubmed
Clinical pregnancy rate , miscarriage rate and healthy baby rate were compared between the MALBAC - NGS - PGT - A and MDA - SNP - PGT - A groups . - pubmed
The results provide greater evidence supporting the wider use of NGS in PGT - A , not only for its lower cost but also for its improved clinical outcomes compared to SNP - based PGT - A. - pubmed
We synthesized 55 analogs based on the chemical structure of 4 - gingerol and evaluated their RhlR inhibitory activities using cell - based reporter strain assay . - pubmed
Comprehensive structure - activity relationship studies identified the alkynyl ketone 30 as the most potent RhlR antagonist . - pubmed
Essential role of calcium in extending RTX adhesins to their target . - pubmed
However , all the tetra - tandemers have at least one calcium ion coordinated in the linker region between beta - sandwich domains whose role appears to be the rigidification of the extender region to help the adhesin extend its reach . - pubmed
METHODS : We used a national sample of administrative claims data to explore associations diagnostic test choice ( TST , IGRA , TST with subsequent IGRA ) and treatment persistence and completion in persons initiating a daily dose isoniazid LTBI treatment regimen in the US private healthcare sector between July 2011 and March 2014 . - pubmed
( Aza ) Acenes Share the C2 Brink with ( Anti)Aromatic Macrocycles - Local vs. - pubmed
Background : The Attain Stability Quadripolar 4798 lead is a relatively new quadripolar active fixation coronary sinus ( CS ) lead . - pubmed
Serum cholesterol correlated poorly with PBI ( r = --0,4427 ) , better correlation was achieved after logarithmic transformation of PBI values ( r = --0,6839 ) . - pubmed
Reference line and covariance between log PBI and serum cholesterol in decreasing thyroid function did not differ significantly from reference line and covariance in steady state , showing that serum cholesterol accompanies closely and without greater delay the changing PBI . - pubmed
Isoprenaline and dopamine also increased phosphorylase a activity . - pubmed
Since both agents stimulate adrenergic beta - receptors in cardiac muscle , the results are concordant with the hypothesis that cAMP is involved as a mediator of the inotropic response to adrenergic beta - stimulation . - pubmed
The enzyme activity was inhibited by reduction with borohydride in the presence of the substrate and was inactivated by carboxypeptidase A [ EC 3.4.12.2 ] digestion . - pubmed
Extensive homology in various properties of the enzyme was observed when it was compared with enzymes from other sources , though some differences could be found in the amino acid composition and in the kinetic properties . - pubmed
The increase in blood pressure could be abolished by intraventricular administration of saralasin , a blocker of angiotensin II receptors . - pubmed
Several single amino acid substitutions in the COOH terminus of IL-6 were found to decrease biological activity significantly . - pubmed
It is concluded that the amphipathical alpha - helix structure in the COOH terminus of IL-6 is critical for ligand receptor interaction . - pubmed
Plasma metallothionein concentrations may prove useful for identifying poor zinc status . - pubmed
However , since hepatic metallothionein concentrations rise in response to stress , plasma metallothionein concentrations are increased in this state when the plasma zinc concentrations are also likely to be low . - pubmed
Thus , measurement of both plasma metallothionein and plasma zinc concentrations could differentiate a low plasma zinc due to dietary zinc deficiency from a low concentration due to stress , infection , or other metabolic conditions . - pubmed
4 - Ipomeanol is a naturally occurring toxin that induces lesions in the lung following its activation to an alkylating metabolite by the pulmonary cytochrome P-450 system . - pubmed
These results suggest that the isozymes of pulmonary cytochrome P-450 that act in concert to metabolize 4 - ipomeanol are relatively insensitive to inhibition by carbon monoxide . - pubmed
beta - Adrenergic receptor binding , using [ 3H ] dihydroalprenolol as the ligand , was saturable , linear , and stereospecific . - pubmed
More rapid maturation of pulmonary beta - receptors and SAM flux in females may be a factor in the female advantage with regard to pulmonary surfactant maturation and the survival of premature neonates . - pubmed
In addition , they suggest that the activated Con A receptors generate very similar signals in the two cell types , and that any perturbations of primary signal transduction to the secondary phosphoinositide and [ Ca2 +]i responses in the S49 phenotype are quantitative rather than qualitative . - pubmed
The ligand binding site of this receptor undergoes denaturation during solubilization in digitonin , Triton X-100 , or 3 -[( 3 - cholamidopropyl ) dimethylammonio ]- 1 - propanesulfonate at detergent concentrations above 0.1 % , which is evident from the loss of high - affinity binding of [ 3H ] PK11195 , a ligand selective for the mitochondrial benzodiazepine receptor . - pubmed
Ethanol - induced phospholipase C activation is inhibited by phorbol esters in isolated hepatocytes . - pubmed
Ethanol causes a transient activation of the phosphoinositide - specific phospholipase C in intact hepatocytes and mimics the action of receptor - mediated agonists [ Hoek , Thomas , Rubin & amp ; Rubin ( 1987 ) J. Biol . - pubmed
Preincubation of the hepatocytes with phorbol esters which activate protein kinase C prevented this effect of ethanol : phorbol ester treatment inhibited the ethanol - induced phosphorylase activation , the increase in intracellular free Ca2 + concentrations measured in quin 2 - loaded hepatocytes , and the changes in concentrations of inositol phosphates , phosphoinositides and phosphatidic acid . - pubmed
Phorbol esters acted in a concentration range where they activate protein kinase C ; phorbol esters that do not activate protein kinase C were not effective in inhibiting the effects of ethanol . - pubmed
The inhibition of ethanol - induced Ca2 + mobilization by phorbol esters was prevented by preincubating the cells with the protein kinase C inhibitors 1 -( 5 - isoquinolinesulphonyl )- 2 - methylpiperazine ( H7 ) and sphingosine . - pubmed
H7 also enhanced the Ca2 + mobilization induced by ethanol in cells that were not pretreated with phorbol esters , indicating that the transient nature of the ethanol - induced Ca2 + mobilization may be due to an activation of protein kinase C caused by the accumulation of diacylglycerol . - pubmed
These data support a model whereby ethanol activates the phosphoinositide - specific phospholipase C , possibly by affecting receptor - G - protein - phospholipase C interactions in the membrane . - pubmed
Angiotensin II ( 100 ng ) , injected into the preoptic area , stimulated the intake of water but not salt , to a lesser extent and with a shorter duration as compared with that induced by renin ( 5 mU ) , injected into the preoptic area . - pubmed
This angiotensin II - induced intake of water was markedly inhibited by [ Sar1 , Ile8 ]- angiotensin II , an angiotensin II receptor antagonist , but not by captopril , an angiotensin - converting enzyme inhibitor , previously administered through the same cannula . - pubmed
The effect of renin was inhibited by [ Sar1 , Ile8 ]- angiotensin II ( 10 micrograms ) and was eliminated by captopril ( 25 micrograms ) injected into the preoptic area . - pubmed
This effect of renin was not influenced by the peripheral administration of captopril . - pubmed
The effect of renin was also inhibited by GABA , muscimol or taurine injected into the cerebroventricle , in larger doses , or into the preoptic area in smaller doses. - pubmed
When tissues were cultured in medium without progesterone , the amounts of dPRL measured in the medium declined steadily over 6 days , regardless of the gestational age of the endometrium . - pubmed
dPRL was consistently measured in medium harvested from cultures that received either progesterone or medroxyprogesterone ; however , progesterone did not induce an increase in the amounts of dPRL released by cultures prepared from early pregnancy endometrium . - pubmed
Experiments were carried out with growing cultures , and the following pathways were demonstrated for metabolism of the growth substrates : ( i ) TMBA produced GA , which was further degraded without the formation of aromatic intermediates ; ( ii ) GA formed pyrogallol , which was stable to further transformation ; and ( iii ) CV was degraded by a series of steps involving de - O - methylation , oxidation of the aldehyde group , and decarboxylation to 3 - chlorocatechol before ring cleavage . - pubmed
Plasma levels of cholesterol , high - density lipoprotein ( HDL ) cholesterol and triglycerides did not differ between the two groups . - pubmed
They also indicate that antidepressant neuroleptics , which are potent serotonin 5 - HT2 and alpha 1 - adrenergic blocking agents , after chronic administration affect the serotonin system in a manner similar to that produced by chronic treatment with classical antidepressant drugs . - pubmed
During primary infections DTH responses to leishmanin antigen were detected as early as 8 weeks PI , and continued up to 27 weeks PI . - pubmed
Dopamine , an endogenous catecholamine , was used alone and in conjunction with selective blockade or adrenergic and dopaminergic receptors . - pubmed
The effect was significantly blocked by the peripherally acting dopaminergic blocker domperidone and by cis - flupenthixol , which blocks both peripheral and central dopaminergic receptors . - pubmed
The effect of dopamine was not significantly altered by the beta 1 - adrenoceptor blocker practolol , the alpha - adrenoceptor blocker phentolamine , or the alpha + beta - adrenoceptor blocker labetalol . - pubmed
Consequently , this study indicates that dopamine acts on gastric antral motility through dopaminergic receptors . - pubmed
Hyper(ADP - ribosyl)ation of histone H1 . - pubmed
Electrophoresis of [ 32P]ADP - ribosylated histones on first - dimension acid - urea or acid - urea - Triton gels and on second - dimension acid -- urea -- cetyltriammonium bromide gels revealed that , of all histones , only histone H1 could be significantly poly ( ADP - ribosyl )ated while only minimal modification could be recovered with histone H1(0 ) . - pubmed
Furthermore , the extent of ADP - ribosylation present on pancreatic histone H1 is shown to proportionally retard this protein 's electrophoretic mobility in all gel systems and to consist of a distinct series of at least 12 modification intermediates which can be evidenced , in nuclei or nucleosomes , and fully recovered along with histone H1 upon its selective extraction with 5 % perchloric acid . - pubmed
The generation of these increasingly ADP - ribosylated forms of histone H1 is also demonstrated to be time dependent and the more complex ADP - ribosylated forms of this histone are favored at high NAD + concentrations . - pubmed
The reflex bradycardia elicited by central vagal nerve stimulation was accentuated by urapidil given intracisternally , but this only occurred in the presence of beta adrenoceptor blockade with propranolol . - pubmed
Independently from methods , acid phosphatase as well as urine hydroxyprolin show elevated levels only in cases of metastatic formation . - pubmed
A significant positive relation between alcohol consumption and lung - cancer incidence was also found , accounted for primarily by an increased risk among subjects who consumed larger amounts of wine or whiskey , as compared with the risks among nonconsumers of these beverages ( relative risk , 2.19 , [ P = 0.03 ] and 2.62 [ P less than 0.01 ] , respectively ) . - pubmed
Lymphokine supernatants induced increased methyl-3H - TdR incorporation in adherent cells in 3 of 8 experiments , and microscopic proliferation of phagocytic cells was evident in one experiment . - pubmed
Promoter activity of the gene encoding the beta - amyloid precursor protein is up - regulated by growth factors , phorbol ester , retinoic acid and interleukin-1 . - pubmed
We analyzed the effects of nerve growth factor ( NGF ) , basic fibroblast growth factor ( bFGF ) , phorbol 12 - myristate 13 - acetate ( PMA ) , interleukin-1 ( IL-1 ) and retinoic acid ( RA ) on promoter activity of the beta APP gene . - pubmed
The treatment of cells with either NGF or bFGF resulted in a higher degree of stimulation in the basal level of promoter activity than when cells were treated with either PMA , IL-1 or RA . - pubmed
Heme - protein interactions in horse heart myoglobin at neutral pH and exposed to acid investigated by time - resolved fluorescence in the pico- to nanosecond time range . - pubmed
The 4822 - ns lifetime was assigned to reversibly heme - dissociated myoglobin . - pubmed
These analyses revealed the presence of three species originating from heme - protein interactions : the native form of crystalline myoglobin , the conformation with disorder hemes , and the reversibly dissociated heme - free myoglobin . - pubmed
Labeling with anti - PCD6 subunit ( of the inositol trisphosphate / ryanodine receptor ) was demonstrated on carotenoid droplets suggesting they possess calcium channels . - pubmed
Subjects have elevated serum thyroid hormone levels in association with a nonsuppressed TSH . - pubmed
Failure to recognize the inappropriate persistence of TSH secretion , in spite of elevated thyroid hormone levels , has commonly resulted in erroneous diagnosis leading to treatment aimed to normalize the thyroid hormone level . - pubmed
To demonstrate promoter activity , a segment containing 611 bp of the promoter region ( from -442 to +169 ) was subcloned in front of a promoterless chloramphenicol acetyltransferase ( CAT ) gene . - pubmed
Alcoholysis of epsilon - decalactone with polyethylene glycol - modified lipase in 1,1,1 - trichloroethane . - pubmed
These results were discussed in relation to carbon number of n - alcohol , optimum temperature and comparison with modified and non - modified lipases . - pubmed
Streptomycin - dependent ( Str - D ) ribosomes almost did not translate the apr mRNA . - pubmed
Overall fat supplementation did not affect digestibilities of DM , ADF , or NDF but decreased digestibility of fatty acids . - pubmed
Tallow , Ca salt of palm fatty acids , and prilled fatty acids did not differ in milk yield response . - pubmed
It was found that the group receiving EPN ( n = 46 ) normalized their nitrogen balance sooner and demonstrated a consistent decrease in their creatine phosphokinase level . - pubmed
Enhancement of IL-13 production by cyclosporin A. - pubmed
In this work , we show that IL-13 is produced optimally by T cells when stimulated with a combination of anti - CD28 and PMA . - pubmed
The effect of CsA did not depend on the presence of PMA ; upon CD3 and CD28 stimulation , CsA equally enhanced IL-13 production . - pubmed
Both naive and memory CD4 + T cells and CD8 + T cells produced IL-13 , and production in all cell types could be enhanced by CsA. - pubmed
In contrast to IL-13 , IL-4 production was observed mainly in CD4 + memory cells , required costimulation through CD3 , and was inhibited by CsA. - pubmed
The finding that IL-13 production is enhanced instead of diminished in the presence of CsA may explain the Th2 - inducing effects of CsA in vivo . - pubmed
Transfections of antisense oligonucleotides or antisense cDNA plasmids into these cell lines using dendrimers resulted in a specific and dose dependent inhibition of luciferase expression . - pubmed
Burial of the hydrophobic amino acids on trimer formation is a strongly conserved feature in the vicilin family . - pubmed
Because the neurotoxicity induced by activation of the NMDA subtype of glutamate receptor is mediated through excessive Ca2 + influx , we investigated whether SRIF counteracted NMDA - induced neuronal cell death . - pubmed
SRIF type 2 receptor agonists , octreotide ( SMS 201 - 995 ; 10 microM ) and vapreotide ( RC 160 ; 10 microM ) , did not influence the NMDA - dependent neurotoxicity . - pubmed
Pertussin toxin ( 300 ng / ml ) , a G protein blocker , antagonized the protective effect of SRIF on NMDA neurotoxicity . - pubmed
Furthermore , the neuroprotective effect of SRIF was mimicked by dibutyryl - cyclic GMP ( 10 microM ) , a cyclic GMP analogue , whereas 8 -( 4 - chlorphenylthio )- cyclic AMP ( 10 microM ) , a cyclic AMP analogue , was ineffective . - pubmed
The cyclic GMP content was increased in a dose - dependent manner by SRIF ( 2.5 - 10 microM ) . - pubmed
Finally , both specific ( Rp-8 - bromoguanosine 3 ' , 5 '- monophosphate , 10 microM ) and nonspecific [ 1 -( 5 isoquinolinylsulfonyl )- 2 - methylpiperazine ( H7 ) , 10 microM ] cyclic GMP - dependent protein kinase ( cGMP - PK ) inhibitors did not interfere with NMDA toxicity but substantially reduced SRIF neuroprotection . - pubmed
Our data suggest a selective neuroprotective role of SRIF versus NMDA - induced nonapoptotic neuronal death in cortical cells . - pubmed
This effect is likely mediated by cGMP - PK presumably by regulation of the intracellular Ca2 + level . - pubmed
LDL was isolated and susceptibility of LDL to V-70 ( 4 - methoxy-2,4 - dimethylvalerinitrile )-mediated oxidation was assessed by measuring conjugated diene production at 234 nm , the lag phase being a measure of the resistance of LDL to oxidation . - pubmed
Alveolar neutrophil oxidative burst and beta2 integrin expression in experimental acute pulmonary inflammation are not modified by inhaled nitric oxide . - pubmed
We found that 18 h after LPS , lung wet , dry , and wet - to - dry weights , H2O2 production , and CD11b / CD18 expression were increased . - pubmed
PMN obtained from BAL were highly activated as evidenced by an already maximal expression of the beta2 integrin CD11b / CD18 , whereas the high H2O2 production at basal state could be further enhanced after ex vivo stimulation . - pubmed
DNA isolated from intact mitochondria treated with DNase consisted of 16.3 kilobase pairs , mostly circular , mitochondrial DNA molecules and a mixture of nuclear and mitochondrial DNA fragments , as identified by agarose gel electrophoresis and hybridization . - pubmed
Effects of mitochondria and o - methoxybenzoylalanine on 3 - hydroxyanthranilic acid dioxygenase activity and quinolinic acid synthesis . - pubmed
The use of o - methoxybenzoylalanine , a selective kynureninase inhibitor , has been proposed with the aim of reducing brain synthesis of quinolinic acid , an excitotoxic tryptophan metabolite . - pubmed
In liver homogenates , however , this compound caused unexpected accumulation of 3 - hydroxyanthranilic acid , the product of kynureninase activity and the precursor of quinolinic acid . - pubmed
To explain this observation , we investigated the interaction(s ) of o - methoxybenzoylalanine with 3 - hydroxyanthranilic acid dioxygenase , the enzyme responsible for quinolinic acid formation . - pubmed
When the purified enzyme or partially purified cytosol preparations were used , o - methoxybenzoylalanine did not affect 3 - hydroxyanthranilic acid dioxygenase activity . - pubmed
It is interesting that addition of purified mitochondria to 3 - hydroxyanthranilic acid dioxygenase preparations reduced the enzymatic activity and the synthesis of quinolinic acid . - pubmed
Cimetidine activates interleukin-12 , which enhances cellular immunity . - pubmed
IIR was activated by hyperpolarisation below the K+ equilibrium potential and could be blocked by submillimolar concentrations of Cs + or Ba2 + , and partially by physiological concentrations of Na + . - pubmed
Several posttranslational modifications of subunits II , III , and IV were observed : ( 1 ) N - terminal methionines of subunits III and IV are split off . - pubmed
PHP is divided into types Ia , b , c and II , depending on the presence of Albright ' s hereditary osteodystrophy ( AHO ) , defective urinary excretion of phosphate ( U - P ) and response in urinary excretion of cyclic adenosine monophosphate ( U - cAMP ) after the administration of exogenous PTH . - pubmed
The dependence of the expression of the tagged loci on the FixL / FixJ oxygen - sensing two - component regulatory system was examined . - pubmed
The remaining seven loci showed fixL- and fixJ - independent induction of expression in response to oxygen limitation . - pubmed
Permeabilized platelets , expressing normal levels of P - selectin , displayed a Ca ( 2 +)-dependent increase in shape change and MLC - P. - pubmed
Thrombin activated RhoA as measured by a rhotekin - binding assay within 30 s of stimulation under conditions of constant [ Ca ( 2 +)]( i ) . - pubmed
Under the same conditions and timecourse , thrombin or GTPgammaS induced an increase in MLC - P and platelet shape change which was not dependent on an increase in [ Ca(2+)](i ) . - pubmed
The thrombin- and GTPgammaS - induced MLC - P in constant [ Ca ( 2 +)]( i ) was inhibited by the addition of Y27632 , a Rho - kinase inhibitor . - pubmed
This study directly demonstrates that thrombin can activate RhoA in platelets in a timecourse compatible with a role in increasing MLC - P and shape change ( not involving an increase in [ Ca(2+)](i ) ) . - pubmed
Fructose feeding resulted in hyperinsulinemia and elevated blood pressure when compared with that in controls ( plasma insulin , 311.3+/-11.4 v control 164.4+/-11.8 pmol / L , P & lt ; .05 ; SBP , 135.4+/-4.2 v control 105.5+/-1.3 mm Hg , P & lt ; .05 ) . - pubmed
Therefore , the antihypertensive effect of L - arginine could be , at least in part , the result of the restoration of plasma insulin levels by its vasodilator ability to increase blood flow to insulin sensitive tissues . - pubmed
FAK antisense oligodeoxynucleotides ( ODNs ) were transfected into SMCs by cationic lipid to investigate its modulatory effects on tyrosine phosphorylation . - pubmed
Three types of interventions were found : technical interventions in the dialysis mechanisms ( increasing the frequency of dialysis sessions and varying the concentration of sodium in the dialysate ) , pharmaceutical interventions ( ACE - inhibitors ) , and a dietetic intervention . - pubmed
AIM : To investigate factors affecting the properties of antisense oligodeoxy nucleotides ( ASON)-liposomes complex and their cellular uptake . - pubmed
All of the drugs promote reductions in the levels of cytochromes b and c in the cells , whilst the Ag ( I ) complex also lowers the amount of cytochrome aa3 . - pubmed
Interestingly , neither the treatment with dexamethasone in vitro nor triggering of apoptosis in vivo up- regulated clusterin expression , opposing the view that clusterin is involved in apoptotic processes . - pubmed
Omapatrilat inhibits both angiotensin - converting enzyme ( ACE ) and neutral endopeptidase ( NEP ) . - pubmed
Both processes affect the abundance of volatile organic compounds ( VOCs ) in the breath because oxidative stress causes lipid peroxidation of polyunsaturated fatty acids in membranes , producing alkanes and methylalkanes which are catabolized by CYP . - pubmed
Breath samples were analyzed by gas chromatography and mass spectroscopy in order to determine the breath methylated alkane contour ( BMAC ) , a three - dimensional display of the alveolar gradients ( abundance in breath minus abundance in room air ) of C4 - C20 alkanes and monomethylated alkanes . - pubmed
The chaperone mini - GroEL is a soluble recombinant fragment containing the 191 - 345 amino acid sequence of GroEL with a 6xHis tag . - pubmed
In a suspended system , mini - GroEL showed significant enhancement of the activity recovery of rhIFN - gamma , applyed with a 1 - 5:1 stoichiometry of mini - GroEL to rhIFN - gamma at 25 degrees C. Moreover , 1 M urea in the renaturation buffer had a synergistic effect on suppressing the aggregation and improving the activity recovery . - pubmed
Measurements included cell recovery , platelet viability according to hypotonic shock response ( HSR ) , platelet aggregation with ADP , morphological and ultrastructural properties of defrozen platelets . - pubmed
Apoptosis of these cells caused by Phx was unlikely to be associated with p53 , Bax , and Bcl-2 , because the levels of these proteins were not altered regardless of the presence or absence of Phx . - pubmed
RESULTS : Two known genes SLC6A1 and SLC6A11 were identified ; as well as the GC content of this genomic sequence was 47 % and 3 putative CpG islands were predicted in the genomic sequence , located in 130,685 - 131,516 bp , 307,090 - 307,870 bp and 415,585 - 416,308 bp , respectively . - pubmed
CONCLUSIONS : The methods , as mentioned above , might be used for annotating the biological information in the genomic sequence , such as gene structure , GC content , CpG island . - pubmed
OCCUPANCY OF ADRENERGIC RECEPTORS AND INHIBITION OF CATECHOL O - METHYL TRANSFERASE BY TROPOLONES . - pubmed
INTERACTION OF PHOSPHOROTHIOATE WITH THE DISULFIDE BONDS OF RIBONUCLEASE AND LYSOZYME . - pubmed
Laser Doppler perfusion ratio ( R / L ) of ligated versus nonligated distal hindlimb increased from 0.06 + /- 0.01 ( immediately after ligation ) to 0.25 + /- 0.02 ( day 7 ) in the PBS group and only from 0.07 + /- 0.02 to 0.13 + /- 0.02 in the mibefradil group ( p & lt;.01 ) . - pubmed
Relative hemoglobin oxygen saturation measurements confirmed these findings ( p & lt;.02 ) . - pubmed
Instead , the mutant assembled efficiently in buffers containing CaCl2 & gt ; or = 6 mM forming filaments that were 10 times longer than those formed by wt NF - L , although their diameter was significantly smaller ( 6 - 7 nm ) . - pubmed
Bacteria were exposed to a nitrogen laser ( average power 2.0 mW ) in vitro at power density of 70 + /- 0.7 W / m2 for 0 - 30 min , and the cell viability was determined by luciferase reporter phage ( LRP ) assay . - pubmed
Electron paramagnetic resonance ( EPR ) studies using 5 - doxyl strearic acid ( 5 - DS ) as a probe suggest alterations in lipid regions of the cell wall . - pubmed
PTH and interactions with bisphosphonates . - pubmed
The failure of the ' washout ' period to restore the normal response to PTH is suggestive of a physico - chemical interaction on the level of the matrix embedded bisphosphonate with the overlaying bone lining cells , rather than of direct effects of the drug on osteoblasts or their precursor cells . - pubmed
Clinical investigators studying anabolic drugs such as PTH should be aware of potential long - term interactions of bisphosphonates when assessing the outcome of their experiments . - pubmed
However , the beneficial effect of bisphosphonates like alendronate on PTH - induced bone remodeling , as well as its potent action in the protection of bone loss after cessation of anabolic therapy might outweigh the worries about a small delay in the bone response to parathyroid hormone . - pubmed
Clinical trial data and real - world experience have shown the nonnucleoside reverse transcriptase inhibitors ( NNRTIs ) to be an important class of agents for the treatment of HIV . - pubmed
The preparation and biological evaluation of 5 - substituted-6 - hydroxy-2 -( anilino ) pyrimidinones as a new class of DNA polymerase IIIC inhibitors , required for the replication of chromosomal DNA in Gram - positive bacteria , are described . - pubmed
Ca6Cu2Sn7 crystallizes in the monoclinic space group C2 / m ( No . - pubmed
In cell - free systems , p53R2 did not oxidize a reactive oxygen species ( ROS ) indicator carboxy - H2DCFDA , but another class I RR small subunit , hRRM2 , did . - pubmed
Further studies showed that purified recombinant p53R2 protein has catalase activity , which breaks down H2O2 . - pubmed
The fluorescence intensity in mutants oxidizing carboxy - H2DCFDA , in order from highest to lowest , was Y331F & gt ; Y285F & gt ; Y49F & gt ; Y241H & gt ; wild - type p53R2 . - pubmed
Of interest , the ability to oxidize carboxy - H2DCFDA indicated by fluorescence intensity was negatively correlated with RR activity from wild - type p53R2 , mutants Y331F , Y285F , and Y49F. - pubmed
A simple , sensitive and specific LC - MS / MS method for the simultaneous determination of sulforaphane ( SFN ) and its major metabolites , the glutathione ( SFN - GSH ) and N - acetyl cysteine conjugates ( SFN - NAC ) from biological matrices was developed and validated . - pubmed
Indo - mo investigation of gas - phase acidities of carbamates and thiocarbamates . - pubmed
He received conventional therapy ( steroids , mycophenolate , anti - IL-2 monoclonal antibodies , anti - thymocyte globulin ) without success . - pubmed
Ischemia promotes deacetylation of core histone proteins H3 and H4 and dimethylation of histone H3 at lysine-9 ( H3 - K9 ) over the MOR-1 promoter , an signature of epigenetic gene silencing . - pubmed
A series of 2 - alkylthio-1 -[ 4 -( 1 - benzyl-2 - ethyl-4 - nitro-1H - - imidazol-5 - yl )- piperazin-1 - yl ] ethanones ( 3 - 9 ) and alkyl -[ 4 -( 1 - benzyl-2 - ethyl-4 - nitro-1H - imidazol-5 - yl )- piperazin-1 - yl ) ketones ( 11 - 20 ) as well as the indole analogue 22 were synthesized from 4 - nitro-5 - piperazinyl imidazole derivative 1 , with the aim to develop newly non - nucleoside reverse transcriptase inhibitors ( NNRTIs ) . - pubmed
A novel molecularly imprinted polymer ( MIP ) selective for tryptophan ( Trp ) was described where polymerization was performed in aqueous media . - pubmed
The results of adsorption experiments indicated that the selectivity of MIP , which was synthesized with bonded beta - CD and AA [ MIP ( 1 ) ] , was superior to those obtained with AA [ MIP ( 2 ) ] or bonded beta - CD [ MIP(3 ) ] . - pubmed
Synthesis of 2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.2 ] nonane , 2 -( pyridin-3 - yl )- 1 - azabicyclo [ 2.2.2 ] octane , and 2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.1 ] octane , a class of potent nicotinic acetylcholine receptor - ligands . - pubmed
In an attempt to generate nicotinic acetylcholine receptor ( nAChR ) ligands selective for the alpha4beta2 and alpha7 subtype receptors we designed and synthesized constrained versions of anabasine , a naturally occurring nAChR ligand . - pubmed
All compounds synthesized exhibited high affinity for the alpha4beta2 nAChR subtype ( K i & lt ; or = 0.5 - 15 nM ) , a subset bound with high affinity for the alpha7 receptor subtype ( K i & lt ; or = 110 nM ) , selectivity over the alpha3beta4 ( ganglion ) receptor subtype was seen within the 2 -( pyridin-3 - yl )- 1 - azabicyclo [ 2.2.2 ] octane series and for the muscle ( alpha1betagammadelta ) subtype in the 2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.2 ] nonane series . - pubmed
An ATR - FTIR experiment showed features at 1632 and 1602 cm(-1 ) that are attributed to NO ( 2 ) complexed to NO ( 3 )(- ) and HNO ( 3 ) , respectively . - pubmed
Community analysis of betaproteobacterial ammonia - oxidizing bacteria using the amoCAB operon . - pubmed
The genes and intergenic regions of the amoCAB operon were analyzed to establish their potential as molecular markers for analyzing ammonia - oxidizing betaproteobacterial ( beta - AOB ) communities . - pubmed
NO - mediated S - nitrosation of cysteine residues has been recognized as a fundamental post - translational modification . - pubmed
Moreover , the nitrosocysteine residue for each protein ( HSPA9/368 , beta - actin / 16 , TMP3 / 170 , vimentin / 328 ) was also determined , and the relative ratio of S - nitrosation / non - S - nitrosation for Cys328 of vimentin was estimated using MASIC software . - pubmed
By the combination of the biotin switch method with 2 - DE and Western blot analysis , S - nitrosoproteins can be screened and characterized by MS , providing a basis for further study of the physiological significance of each S - nitrosoproteins . - pubmed
Instead , we show that nitrite enhances the ATP release from RBCs , which is known to produce vasodilation by several different methods including the interaction with purinergic receptors on the endothelium that stimulate the synthesis of NO by endothelial NO synthase . - pubmed
The observed decrease in blood pressure was not observed if endothelial NO synthase was inhibited by N ( omega )- nitro - L - arginine methyl ester ( L - NAME ) or when any released ATP was degraded by apyrase . - pubmed
The nitrite - enhanced ATP release was shown to involve an increased binding of nitrite - modified hemoglobin to the RBC membrane that displaces glycolytic enzymes from the membrane , resulting in the formation of a pool of ATP that is released from the RBC . - pubmed
The phosphatase KdsC cleaves 3 - deoxy - D - manno - octulosonate 8 - phosphate to generate a molecule of inorganic phosphate and Kdo . - pubmed
KdsC belongs to the broad haloacid dehalogenase superfamily . - pubmed
It is therefore not clear why KdsC , which lacks a cap domain , is catalytically efficient and highly specific to 3 - deoxy - D - manno - octulosonate 8 - phosphate . - pubmed
When treated in vivo with cyclophosphamide , tumors coexpressing Mcl-1 and Myc transgenes were significantly more resistant than conventional Emu - Myc lymphomas . - pubmed
By using the tripodal ligand ntb ( tris ( benzimidazole-2 - ylmethyl ) amine ) and lanthanide nitrate , three isomorphous series of coordination frameworks of the general formula [ Ln ( ntb )( NO ( 3 ))( 3 ) ] solvents ( series 1 : monoclinic C2 / c , Ln = Gd ( 3 + ) and Yb ( 3 + ) ; series 2 : hexagonal P3(1)/c , Ln = Nd ( 3 + ) , Eu ( 3 + ) , Gd ( 3 + ) , and Er ( 3 + ) ; series 3 , cubic Pa3 , Ln = Gd ( 3 + ) and Er ( 3 + ) ; solvent = H ( 2 ) O or CH ( 3 ) OH ) have been assembled and characterized with IR , elemental analyses , and single crystal and powder X - ray diffraction methods . - pubmed
These findings support clinical development of daratumumab for the treatment of CD38 - positive MM tumors . - pubmed
p27 gene encodes a lipoprotein that binds triacylated glycolipids and modulates the host immune responses by inhibiting the MHC - II Ag processing . - pubmed
pneumoniae HMGR with K(i ) values in the range of 6.45 - 20.49 muM than the statin drug lovastatin ( K(i)=116.25 muM ) , a classical inhibitor of class I HMGR . - pubmed
In some cases , I prostanoid ( IP ) receptor and E prostanoid receptor antagonists were added before the PGI2 analog treatment . - pubmed
T - cell polarization function was investigated by measuring interferon gamma , interleukin 13 ( IL-13 ) , and IL-17A production by T cells cocultured with iloprost - treated MDDCs . - pubmed
RESULTS : Iloprost and treprostinil suppressed LPS - induced TNF - alpha expression in MDDCs . - pubmed
This effect could be reversed by an IP receptor antagonist , CAY10449 , but not by E prostanoid receptor antagonists . - pubmed
Iloprost suppressed the LPS - induced expression of costimulatory molecules , including CD80 , CD86 , CD40 , and HLA - DR . - pubmed
Iloprost - treated MDDCs increased IL-17A production by T cells . - pubmed
Automated sequence analysis of exons and intron - exon boundaries did not reveal any genetic variation in the NOTCH3 gene , and electron microscopy excluded the presence of GOM . - pubmed
Hexose transporter GLUT1 harbors several distinct regulatory binding sites for flavones and tyrphostins . - pubmed
The facilitative hexose transporter GLUT1 activity is blocked by tyrosine kinase inhibitors that include natural products such as flavones and isoflavones and synthetic compounds such as tyrphostins , molecules that are structurally unrelated to the transported substrates [ Vera , et al . - pubmed
Here we analyzed the interaction of GLUT1 with quercetin ( a flavone ) , genistein ( an isoflavone ) , and tyrphostin A47 and B46 to evaluate if they share one common or have several binding sites on the protein . - pubmed
Genistein protected GLUT1 against iodide - elicited fluorescence quenching and also decreased the affinity of d - glucose for its external binding site , while quercetin and tyrphostins B46 and A47 promoted fluorescence quenching and did not affect the external d - glucose binding site . - pubmed
These findings are explained by a carrier that presents at least three binding sites for tyrosine kinase inhibitors , in which ( i ) genistein interacts with the transporter in a conformation that binds glucose on the external surface ( outward - facing conformation ) , in a site which overlaps with the external binding site for d - glucose , ( ii ) quercetin and tyrphostin B46 interact with the GLUT1 conformation which binds glucose by the internal side of the membrane ( inward - facing conformation ) , but to a site accessible from the external surface of the protein , and ( iii ) the binding site for tyrphostin A47 is accessible from the inner surface of GLUT1 by binding to the inward - facing conformation of the transporter . - pubmed
The concordance for subject classification according to the National Cholesterol Education Program ( NCEP ) guidelines was significant ( P & lt ; 0.001 ) , with substantial agreement for TC ( K(w ) = 0.67 ) , and moderate agreement for TG ( K(w ) = 0.50 ) . - pubmed
Mutation of plasma membrane Ca2 + ATPase isoform 3 in a family with X - linked congenital cerebellar ataxia impairs Ca2 + homeostasis . - pubmed
Membrane transporters , among them the calmodulin ( CaM )- activated plasma membrane Ca ( 2 + ) ATPases ( PMCAs ) that extrude Ca ( 2 + ) from the cell , play a key role in neuronal Ca ( 2 + ) homeostasis . - pubmed
The mutation reduced the ability of the PMCA3 pump to control the cellular homeostasis of Ca ( 2 + ) . - pubmed
In addition , eugenol stimulated PAF - acetylhydrolase activity suggesting that inhibition of PAF could be due to its inactivation to lyso - PAF . - pubmed
Retinaldehyde dehydrogenase 3 ( RALDH3 ) is the key enzyme required to generate retinoic acid within the olfactory epithelium . - pubmed
PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria . - pubmed
Although Sulfurovum - like soxB genes were detected in all fluid samples , the RNA profiles were nearly identical among the vents and suggest that Sulfurimonas - like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent . - pubmed
Community patterns of subseafloor Epsilonproteobacteria 16S rRNA genes were best matched to methane concentrations in vent fluids , as well as individual vent locations , indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations . - pubmed
Formation of cell surface FHC dimers is critically dependent on an unpaired reactive cysteine 67 in the alpha1 helix of the class I heavy chain . - pubmed
Sorafenib is an orally administered active multikinase inhibitor for metastatic renal cell carcinoma that is now considered a standard agent . - pubmed
OBJECTIVE : The aim of the study was to compare annual bone mineral density ( BMD ) changes among new users of selective serotonin reuptake inhibitors ( SSRIs ) , new users of tricyclic antidepressants ( TCAs ) , and nonusers of antidepressant medications . - pubmed
RESULTS : BMD was compared among 311 new users of SSRIs , 71 new users of TCAs , and 1590 nonusers . - pubmed
After adjustment for potential confounders , including age , race , body mass index , menopausal status , and hormone therapy use , mean lumbar spine BMD decreased on average 0.68 % per year in nonusers , 0.63 % per year in SSRI users ( P = .37 for comparison to nonusers ) , and 0.40 % per year in TCA users ( P = .16 for comparison to nonusers ) . - pubmed
METHODS : To avoid ambiguities owing to the heterogeneous nature , we experimented with hESC - VCMs , purified on the basis of zeocin resistance and signature ventricular action potential after LV - MLC2v - tdTomato - T2A - Zeo transduction . - pubmed
RESULTS : Ca ( 2 + ) sparks that were sensitive to inhibitors of sarco / endoplasmic reticulum Ca ( 2 +)- ATPase ( thapsigargin and cyclopiazonic acid ) and ryanodine receptor ( RyR ; ryanodine , tetracaine ) but not inositol trisphosphate receptors ( xestospongin C and 2 - aminoethyl diphenylborinate ) could be recorded . - pubmed
In a permeabilization model , we further showed that RyRs could be sensitized by Ca ( 2 + ) . - pubmed
Furthermore , RyR - mediated Ca ( 2 + ) release sensitized nearby RyRs , leading to compound Ca ( 2 + ) sparks . - pubmed
By contrast , removal of external Na (+ ) or the addition of the Na (+)- Ca ( 2 + ) exchanger inhibitor ( KB - R7943 and SN-6 ) had no effect , suggesting that the Na (+)- Ca ( 2 + ) exchanger is not involved in triggering sparks . - pubmed
In the above backdrop , we report the synthesis of a novel , stable , cross - linked polyester with salicylic acid ( SA ) incorporated in the polymeric backbone and propose a simple synthesis route by melt condensation . - pubmed
The combination of NMR and FT - IR spectral techniques established the ester linkages in the as - synthesized SA - based polyester . - pubmed
The pH - dependent degradation rate and the rate of release of salicylic acid from the as - synthesized SA - based polymer were studied at physiological conditions in vitro . - pubmed
Our results strongly suggest that SA - based polyester supports cell proliferation for 3 days in culture and do not cause cell death ( & lt;7 % ) , as quantified by propidium iodide ( PI ) stained cells . - pubmed
Novel immobilization of a quaternary ammonium moiety on keratin fibers for medical applications . - pubmed
After each resistance exercise session , subjects were randomly divided into two groups with respect to intake of protein ( 0.3 g / kg body weight ) and carbohydrate ( 0.8 g / kg body weight ) immediately after ( P0 ) or 6 h ( P6 ) after the session . - pubmed
We assessed whole - body protein metabolism by measuring nitrogen balance at P0 and P6 on the last 3 days of exercise training . - pubmed
RESULTS : The nitrogen balance was significantly lower in the trained men than in the untrained men at both P0 ( P & lt;0.05 ) and P6 ( P & lt;0.01 ) . - pubmed
The nitrogen balance in trained men was significantly higher at P0 than at P6 ( P & lt;0.01 ) , whereas that in the untrained men was not significantly different between the two periods . - pubmed
Specific inhibitors of the ERK or NF - kappaB pathway ( PD98059 or Bay11 - 7082 ) suppressed TNF - alpha - induced MMP-2 expression in leiomyoma SMCs . - pubmed
The wound - healing assay revealed that TNF - alpha promoted the migration of cultured leiomyoma SMCs ( P = 0.036 ) ; however , PD98059 compromised the cell migration triggered by TNF - alpha . - pubmed
WIDER IMPLICATIONS OF THE FINDINGS : Considering the positive effect of TNF - alpha on leiomyoma SMC migration , strategies targeting TNF - alpha , in parallel with the production of more specific inhibitors of MMPs , may provide alternative therapeutic approaches for the treatment of leiomyoma . - pubmed
In contrast , the Cyanide - Insensitive Oxidase type may have differentiated into recognizable subtypes after another gene cluster duplication . - pubmed
[ Application of diagnosticum based on functionalized carbon nanoparticles for monitoring of affine immunoglobulin purification ] . - pubmed
IgG detection sensitivity using the carbon - G - protein diagnosticum was 80 ng / mL. - pubmed
In the nodules , bacteria differentiate into bacteroids with the ability to fix atmospheric N2 via nitrogenase activity . - pubmed
As nitrogenase is strongly inhibited by oxygen , N2 fixation is made possible by the microaerophilic conditions prevailing in the nodules . - pubmed
Chronic ethanol consumption is known to downregulate expression of the major glutamate transporter 1 ( GLT-1 ) , which increases extracellular glutamate concentrations in subregions of the mesocorticolimbic reward pathway . - pubmed
While beta - lactam antibiotics were initially identified as potent upregulators of GLT-1 expression , only ceftriaxone has been extensively studied in various drug addiction models . - pubmed
In addition , only cefoperazone significantly inhibited hepatic aldehyde dehydrogenase-2 enzyme activity . - pubmed
Chemicals that induce cytochrome P450 ( P450 ) 1A2 , a major enzyme involved in the bioactivation of HAAs , also form in cooked meat . - pubmed
Therefore , well - done cooked meat may pose an increase in cancer risk because it contains both inducers of P450 1A2 and procarcinogenic HAAs . - pubmed
The mean P450 1A2 activity , assessed by caffeine metabolic phenotyping , ranged from 6.3 to 7.1 before the feeding study commenced and from 9.6 to 10.4 during the meat feeding period : the difference in means was significant ( P & lt ; 0.001 ) . - pubmed
Unaltered PhIP , MeIQx , and their P450 1A2 metabolites , N ( 2 )-( beta-1 - glucosiduronyl )- 2 -( hydroxyamino )- 1 - methyl-6 - phenylimidazo [ 4,5 - b ] pyridine ( HON - PhIP - N ( 2 )- Gl ) ; N3 -( beta-1 - glucosiduronyl )- 2 -( hydroxyamino )- 1 - methyl-6 - phenylimidazo [ 4,5 - b ] pyridine ( HON - PhIP - N3 - Gl ) ; 2 - amino-3 - methylimidazo -[ 4,5 - f ] quinoxaline-8 - carboxylic acid ( IQx-8 - COOH ) ; and 2 - amino-8 -( hydroxymethyl )- 3 - methylimidazo [ 4,5 - f ] quinoxaline ( 8 - CH2OH - IQx ) were measured in urine during days 2 , 14 , and 28 of the meat diet . - pubmed
However , there was no statistically significant correlation between the caffeine P450 1A2 phenotype and any urinary HAA biomarker . - pubmed
The caffeine metabolic phenotype for P450 1A2 was a poor predictor of oxidative urinary metabolites of PhIP and MeIQx and may not be a reliable measure to assess the role of HAAs in cancer risk . - pubmed
The accumulation of PrPSc in cells persistently infected with the prion strain Fukuoka-1 ( FK ) , derived from a patient with Gerstmann - Straussler - Scheinker syndrome , was significantly increased in cultures treated with the macroautophagy inhibitor 3 - methyladenine ( 3MA ) but substantially reduced in those treated with the macroautophagy inducer rapamycin . - pubmed
By contrast , neither rapamycin nor 3MA had any apparently effect on PrPSc from either the 22L or the Chandler strain , indicating that the degradation of PrPSc in host cells might be strain - dependent . - pubmed
At 3d , DCE increased infarct size ( CMR : 18.8 % vs. 14.5 % , p = 0.04 ; serum troponin - I : 13.3 vs. 6.9 ng / uL , p & lt ; 0.05 ) and MMP-2 activity levels ( 0.81 vs. 0.49 , p = 0.002 ) , with reduced activation of Akt ( 0.06 versus 0.26 , p = 0.02 ) . - pubmed
The significance of the later remodelling changes ( ventricular thinning and transmurality ) following DCE , possibly due to changes in MMP-2 activity and Akt activation , merits further study . - pubmed
Alanine and aspartate aminotransferases ( ALT , AST ) and creatine kinase ( CK ) activities were measured spectrophotometrically at 340 nm after acrosome reaction with P4 . - pubmed
Acrosome reaction induction by P4 required different creatine kinase activity with the same oxygen consumption and transaminases level to maintain oxidative metabolism and redox state through reducing equivalents transfer between cytosolic and mitochondrial compartment . - pubmed
These data show that ATP binds Dbp5 weakly in rapid equilibrium with a binding affinity ( KT~4 mM ) comparable to the KM for steady - state cycling , while ADP binds an order of magnitude more tightly ( KD~0.4 mM ) . - pubmed
The growing industrial application of imidazolium - type ionic liquids ( ITILs ) is likely to result in their release to the environment . - pubmed
We studied the effects of beta - adrenoceptor antagonists propranolol and nadolol and L - type Ca ( 2 +)- channel blocker verapamil on cardiac reperfusion injury developed after 45 - min coronary occlusion . - pubmed
Suppression of Nfib expression in cell lines derived from the doxycycline - inducible Rb / p53 / TET - Nfib model led to increased apoptosis and suppression of proliferation . - pubmed
Photoactivatable - Ribonucleoside - Enhanced Crosslinking and Immunoprecipitation ( PAR - CLIP ) in combination with next - generation sequencing is a powerful method for identifying endogenous targets of RNA - binding proteins ( RBPs ) . - pubmed
Positive matrix factorization ( PMF ) with the multi - linear engine ( ME-2 ) solver identified six organic aerosol ( OA ) factors , including hydrocarbon - like OA ( HOA ) , coal combustion OA ( CCOA ) , cooking - related OA ( COA ) , biomass burning OA ( BBOA ) and two oxygenated OA ( OOA1 and OOA2 ) , which accounted for 8.5 % , 20.2 % , 18.6 % , 12.4 % , 17.8 % and 22.5 % of the total organics mass on average , respectively . - pubmed
Drug combination approach to overcome resistance to EGFR tyrosine kinase inhibitors in lung cancer . - pubmed
Results showed that acupuncture was an effective treatment method in inhibiting inflammatory conditions , serum IgE levels , and expression of proinflammatory cytokine Th2 ( IL-4 , IL-6 ) , and Th2 ( IL-1beta , TNF - alpha ) mRNA compared with DNCB treatment . - pubmed
BACKGROUND : Autosomal recessive distal renal tubular acidosis ( dRTA ) is a rare hereditary disease caused by pathogenic variants in the ATP6V0A4 gene or ATP6V1B1 gene , and characterized by hyperchloremic metabolic acidosis with normal anion gap , hypokalemia , hypercalciuria , hypocitraturia and nephrocalcinosis . - pubmed
Uric acid , albumin , vitamins are some of non enzymatic molecular antioxidants . - pubmed
Parabens are known to be weak endocrine disruptors because they interfere with the function of endogenous hormones through binding to estrogen receptors . - pubmed
Parabens were separated using a Waters BEH C18 column ( 2.1 mm x 100 mm , 1.7 mum ) and a mobile phase consisting of 10 mM ammonium acetate in water and acetonitrile with a gradient program at a flow rate of 300 muL / min . - pubmed
Efficacy and safety of bispecific T - cell engager ( BiTE ) antibody blinatumomab for the treatment of relapsed / refractory acute lymphoblastic leukemia and non - Hodgkin ' s lymphoma : a systemic review and meta - analysis . - pubmed
Measurement of vitamin D , iron , zinc , thyroid hormones , and prolactin are optional but recommended . - pubmed
( vi ) Treatment of FPHL should start with minoxidil ( 5 % ) , adding 5alpha - reductase inhibitors or antiandrogens when there is severe hair loss or hyperandrogenism . - pubmed
Furthermore , IONP - PEG activated in vitro , the complement system and induced a substantial increment of C3a and C4a anaphylatoxins while IONP - bare and IONP - PVP did not . - pubmed
In vivo IONP - PEG induced an increment in complement activation markers ( C5a and C5b-9 ) , and proinflammatory cytokines ( IL-1beta , IL-6 , TNF - alpha ) . - pubmed
In fungi , green light is absorbed by rhodopsins , opsin proteins carrying a retinal molecule as chromophore . - pubmed
Also , UmOps1 showed a pronounced , concentration - dependent enhancement of pump current caused by weak organic acids ( WOAs ) , especially by acetic acid and indole-3 - acetic acid ( IAA ) . - pubmed
MTAP Loss Promotes Stemness in Glioblastoma and Confers Unique Susceptibility to Purine Starvation . - pubmed
As a combined consequence of purine production deficiency in MTAP - null GBM and the critical dependence of GSCs on purines , the enriched subset of CD133 + cells in MTAP - null GBM can be effectively depleted by inhibition of de novo purine synthesis . - pubmed
TECA , AST and the TECA+AST reatments inhibited the expression of iNOS and COX-2 ; NF - kappaB activity ; and the release of TNF - alpha , IL-6 and IgE. - pubmed
CONCLUSIONS : Our results demonstrate that the combination of TECA and AST could be a promising therapeutic agent for AD by inhibiting NF - kappaB signaling . - pubmed
By chemical methods and GC , GC - MS , 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of d - galactopyranose residues linked by beta-1,6 - glycosidic bonds as a main chain , and the side chain was represented by alpha - arabinose , beta - galactose and 4 - O - methylglucuronic acid . - pubmed
LncRNA - MALAT1 mediates cisplatin resistance via miR-101 - 3p / VEGF - C pathway in bladder cancer . - pubmed
However , the roles and underlying molecular mechanisms of MALAT1 in cisplatin resistance of the BC cells remain largely unclear . - pubmed
Secondly , we found that suppression of MALAT1 enhanced the chemotherapeutic drug sensitivity and inhibited the cisplatin resistance of the BC cells . - pubmed
Thirdly , we showed that MALAT1 affected the cisplatin resistance of the BC cells via regulating the miR-101 - 3p / VEGF - C pathway . - pubmed
In summary , this study demonstrates that MALAT1 , miR-101 - 3p and VEGF - C form a regulatory axis to affect the chemo - resistance of BC cells to CDDP , and provides novel potential targets for treatment of BC . - pubmed
Furthermore , we found that coixol inhibits mitogen - activated protein kinases ( MAPKs ) , nuclear transcription factor kappa B ( NF - kappaB ) pathways , and NOD - like receptor protein ( NLRP ) 3 inflammasome activation . - pubmed
Estrogen receptors ( ERs ) are steroid hormone receptors with important functions in neurogenesis and synaptic plasticity . - pubmed
Carbogen breathing significantly increased VF in BG and WM , and rCNR in thalamus , BG , and WM compared to air breathing . - pubmed
The COP - QP - TC was successfully applied for the SPME of some PAHs and their derivatives from environmental water samples with good method recoveries ranging from 85.8 % to 114.4 % . - pubmed
Sre1 directly regulates genes related to ergosterol biosynthesis as well as genes related to the mevalonate pathway , which synthesizes the building blocks of isoprenoids , including carotenoids . - pubmed
dendrorhous is regulated by Sre1 through the regulation of the mevalonate pathway and the regulation of the crtE and crtR genes . - pubmed
As the crtR gene encodes a cytochrome P450 reductase , Sre1 regulates pathways that include cytochrome P450 enzymes , such as the biosynthesis of carotenoids and sterols . - pubmed
OBJECTIVE : To evaluate the role of PET / CT to visualize the immune - related adverse events ( irAEs ) following pembrolizumab . - pubmed
INTERVENTION : PET / CT before and after neoadjuvant pembrolizumab , before radical cystectomy . - pubmed
OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS : We analyzed the occurrence of irAEs , evaluated according to the Common Terminology Criteria for Adverse Events ( CTCAE ) version 5.0 , against the development of inflammatory FDG uptake described at PET / CT ( irAEs + PET / CT ) . - pubmed
Logistic regression analyses evaluated the association between irAEs + PET / CT and the pathological response to pembrolizumab . - pubmed
RESULTS : Compared with baseline , EPA and DHA supplementation differently modulated LPS - stimulated monocyte cytokine expression . - pubmed
EPA lowered TNFA ( p < 0.001 ) whereas DHA reduced TNFA ( p < 0.001 ) , IL6 ( p < 0.02 ) , MCP1 ( p < 0.03 ) , and IL10 ( p < 0.01 ) . - pubmed
DHA lowered IL10 expression relative to EPA ( p = 0.03 ) . - pubmed
Relative to baseline , EPA , but not DHA , decreased the ratios of TNFA / IL10 and MCP1 / IL10 ( both p < 0.01 ) . - pubmed
Different groups of PUFA derivatives mediated the differential effects of EPA and DHA on monocyte cytokine expression . - pubmed
CONCLUSIONS : EPA and DHA had distinct effects on monocyte inflammatory response with a broader effect of DHA in attenuating pro - inflammatory cytokines . - pubmed
In vivo recordings of Schaffer collateral stimulation - induced synaptic responses during IH in the CA1 region of the hippocampus revealed pathological late phase hypoxic long term potentiation ( hLTP ) ( 154 % ) that lasted more than four hours and could be reversed by depotentiation with low frequency stimulation ( LFS ) , or abolished by NMDA and PKA inhibitors ( MK-801 and CMIQ ) . - pubmed
Mechanism of Two Typical Binders BR and F2604 on Thermal Decomposition of HMX . - pubmed
DSC - TG - FTIR - MS coupling technology was used to study the mechanism of two typical binders , that is , BR and F2604 , on the thermal decomposition behavior of the HMX crystal . - pubmed
Especially in the case of HMX / BR particles , the decomposition temperature is the lowest , but the activation energy is the highest . - pubmed
Based on the results of DSC - TG - FTIR - MS , it is found that the rapid mechanism of binder and active intermediate products inhibits the reaction of relatively inert intermediate products and prolongs the continuous generation time of gas products in the composite particles , which delays the decomposition of HMX to a certain extent . - pubmed
Neurotransmitters involved in ACTH secretion : catecholamines . - pubmed
The presence of these enzyme activites would allow D - fructose to be degraded by the Embden - Meyerhof pathway and/or the Entner - Doudoroff pathway . - pubmed
Glucose 15 mM did not further enhance the cAMP response , although this concentration increased insulin secretion more than two - fold over values observed at glucose 10 mM. - pubmed
Thus , the glucose dose - response relations for cAMP levels and insulin secretion appear to be different , indicating that factors other than cAMP alone determine the secretory response to glucose . - pubmed
Fasting for 24 and 72 h progressively inhibited glucose - induced insulin secretion . - pubmed
Fasting for 24 h shifted the glucose dose - response curves for cAMP and insulin secretion to the right , but the maximum responses at glucose 37.5 mM were not significantly inhibited . - pubmed
The results support the view that inhibition of the adenylate cyclase - cAMP system is a major determinant factor in fasting - induced impairment of insulin secretion during the initial 30 min of glucose stimulation . - pubmed
NADPH - cytochrome c reductase ( EC 1.6.2.3 ) was solubilized and purified 4 - 6 fold by three methods with use of sonication and detergent digestion followed by either DEAE - cellulose chromatography or ammonium sulfate fractionation . - pubmed
Reductase fractions prepared by sodium cholate digestion of microsomes were highly active in supporting the hydroxylation activity in the reconstituted systems , whereas those prepared with sodium deoxycholate were not . - pubmed
These filaments are distinct from the actin microfilament bundles visualized by an antibody against actin , and they are not affected by brief treatment with cytochalasin B. - pubmed
In response to the drugs colcemid and vinblastine , the filaments reacting with this preimmune serum condense to form a compact perinuclear coil of fibers , a distribution and behavior in agreement with that previously described for the 10 nm or intermediate filaments studied by electron microscopy . - pubmed
At the same time , certain doses of each of the platinum compounds induced selective recovery of the preantigen reactive cells ( P - PFC ) and myelocytic progenitor cells ( CFC ) in the bone marrow with a pattern that was unique for each compound . - pubmed
Effects of melanocyte - stimulating hormone ( MSH ) and melatonin on passive avoidance and on an emotional response . - pubmed
The present experiment investigated the opposite effects of synthetic alpha - MSH and Melatonin on acquisition and extinction of a passive avoidance response ( PAR ) and on emotionality , as indexed by defecation , in the PA box . - pubmed
It was found that intraperitoneal ( IP ) administration of alpha - MSH delayed extinction and increased defecation responses whereas IP administration of Melatonin facilitated extinction of the PAR and decreased defecation . - pubmed
The present experiment confirmed MSH - Melatonin opposition on memory and on the defecation response . - pubmed
Since the Dw4 , Dw14 , Dw15 , and DR1 molecules have similar antigen - binding sites and since combinations of these alleles particularly predispose to severe RA , we suggest that synergistic mechanisms are involved . - pubmed
Nucleotide sequence of rabbit elongation factor 1 alpha cDNA . - pubmed
Previous studies have reported elevated interleukin 1 ( IL-1 ) , prostaglandin E2 ( PGE2 ) , and collagenase in this tissue . - pubmed
2 ) The greatest skin response with regard to histamine was found for DFA ( 52 % ) , DPT ( 44 % ) , HD ( 22 % ) , GD ( 10 % ) , AS ( 4 % ) and GF ( 2%). - pubmed
It is possible that longer retention of captopril in the plasma , evidence by larger AUC , may reflect less captopril available to modulate renin activity in the kidney . - pubmed
It was found that heparin concentration of 3 to 500 U per 1 kg collagen causes a hyperadhesive effect on blood platelets and facilitates the formation of the primary thrombocytic thrombus . - pubmed
Clindamycin , which enters phagocytes by the cell membrane adenosine ( nucleoside ) transport system , had only a modest effect on FMLP - mediated superoxide production but inhibited the microbial particle - induced response by approximately 50 % . - pubmed
Dideoxy sequencing of RNA using reverse transcriptase . - pubmed
Serology , absorption experiments and family studies indicate that SNA antigen is a Bw6 associated subtype of B5 antigen closely related to but clearly distinct from each of the B5 CREG antigens . - pubmed
Blockade of morphine - induced increases in brain tryptophan hydroxylase activity by systemic pretreatment with CCK-8 : no reversal by vagotomy . - pubmed
We have investigated the possibility that peripherally administered CCK-8 can also suppress a neurochemical response to opiates , namely the increase in brain tryptophan hydroxylase ( TrpH ) activity and tissue 5 - hydroxyindole acetic acid ( 5 - HIAA ) which results from morphine administration . - pubmed
roughly doubled brain TrpH activity and tissue 5 - HIAA 40 min after injection , pretreatment with CCK-8 ( 5 micrograms / kg i.p . - pubmed
Doses of CCK-8 as low as 1 microgram / kg were effective in blunting the morphine - induced increase in cortical TrpH activity , but the desulfated form was ineffective at doses of 5 and 100 micrograms / kg . - pubmed
Alpha - difluoromethylornithine ( DFMO ) , an irreversible inhibitor of ornithine decarboxylase , has been demonstrated to be an effective agent against a variety of parasitic protozoa but not against Leishmania spp . - pubmed
Hydralazine - induced systemic lupus erythematosus : influence of HLA - DR and sex on susceptibility . - pubmed
26 patients with systemic lupus erythematosus ( SLE ) induced by treatment with the antihypertensive drug hydralazine were investigated to determine if predisposition to the toxic effect was associated with an HLA - DR antigen . - pubmed
The frequency of HLA - DR4 ( 73 % ) was significantly higher in the group with hydralazine - induced SLE than in the other groups ( respectively 33 % , 25 % , and 25 % ) . - pubmed
If the slow acetylators treated with hydralazine were analysed as one group , it was observed that all women with DR4 developed hydralazine - induced SLE ; the only men to do so were those with DR2 who were receiving 200 mg hydralazine per day . - pubmed
These observations have led us to suggest guide lines for hydralazine therapy and point to a striking association between an HLA - DR antigen and an adverse reaction to a therapeutic agent . - pubmed
It was also noted that the distribution of DR antigens in the hydralazine - SLE patients was significantly different from that in the group with idiopathic SLE . - pubmed
Plasma extracted using leached silica glass ( Vycor ) caused inhibition of tracer binding which diluted in parallel to synthetic DYN suggesting the presence of substantial amounts of DYN - like immunoreactivity ( irDYN ) in plasma . - pubmed
Although use of Seppak C18 cartridges resulted in reliable extraction of synthetic porcine DYN from acidified plasma , we have not detected irDYN in any plasma so far studied using this technique . - pubmed
No significant change in the density of [ 3H ] dihydroalprenolol - labeled beta receptors was evident at this time point in either atrial or ventricular tissue , although isoproterenol did decrease binding site density after 7 days of infusion . - pubmed
These results indicate selective desensitization of cardiac beta receptors without changes in alpha-1 receptors by prolonged in vivo stimulation with catecholamines . - pubmed
The cyclic AMP - dependent phosphorylation of pyruvate kinase as a model in the study of regulation and turnover of phosphorylatable proteins . - pubmed
The regrowth of resistant variants could be monitored by luciferase assay of intracellular ATP in cultures incubated with gentamicin ( less than or equal to 2 micrograms / ml ) . - pubmed
These findings of the specific regional localization suggest that PP or PP - LI may have a physiological role in the central nervous system . - pubmed
Effects of substitution of the absolutely invariant glutamine-4 and phenylalanine-5 in bovine pancreatic phospholipase A2 on enzymatic activity and substrate binding properties . - pubmed
Aortic wall cholesterol content ( on a weight basis ) correlated positively with serum cholesterol concentration ( r = + 0.69 , P approximately 0.05 ) and negatively with the ratio of HDL cholesterol to ( LDL plus VLDL ) cholesterol ( double log plot : r = -0.79 , P less than 0.025 ) . - pubmed
A study of plasma neurophysins and action of morphine ] . - pubmed
Morphine administration was followed by a reduction of the diuresis and a slight elevation of plasmatic neurophysins . - pubmed
DOM and related 2,5 - dimethoxy-4 - alkylphenylisopropylamines : behavioral and serotonin receptor properties . - pubmed
The fundus preparation could not be used to determine pD2 values because all of the compounds were found to interact in an agonistic manner both with serotonin and PRT ( phenoxybenzamine resistant tryptamine ) receptors . - pubmed
While each of these agents possesses a high 5 - HT receptor affinity , and while their behavioral effects might , therefore , involve a serotonergic component , the stimulus properties of these compounds are qualitatively dissimilar to those produced by the training dose of 5 - OMe DMT . - pubmed
These data may identify a role for carbonic anhydrase in potassium excretion by newborn similar to that of adults . - pubmed
Thus , in the current study , we investigated whether BMS-186511 , a bisubstrate analogue inhibitor of FT , would inhibit the malignant growth properties of a cell line established from malignant schwannoma of an NF1 patient . - pubmed
BMS-186511 was found to specifically inhibit FT , but not geranylgeranyltransferase I , a closely related enzyme . - pubmed
Concentration - response experiments showed that the response to exogenous nitric oxide ( NO ; 10(-7)-10(-4 ) M ) was unaffected by carbachol ( 10(-6 ) M ) preincubation , whereas responses to vasoactive intestinal polypeptide ( VIP ) and ATP were significantly reduced. - pubmed
Neonatal exposure to xenobiotics alters adult hepatic protein kinase C alpha levels and testosterone metabolism : differential effects by diethylstilbestrol and phenobarbital . - pubmed
The PKC alpha levels generally correlated ( r = -.75 ) with 16 alpha - hydroxytestosterone formation in all samples . - pubmed
A systematic mutational analysis of this 74 - amino acid protein was performed and the effects on the potency of receptor binding and of C5a - induced intracellular calcium ion mobilization were measured . - pubmed
Serum creatinine and CrCL measurements during the CsA challenge test predicted post - PTx levels : SCr 1.6 + /- 0.1 vs. 1.7 + /- 0.3 mg / dl , P = 0.48 , and CrCl 68 + /- 10 vs. 53 + /- 3 ml / min , P = 0.17 , respectively . - pubmed
The mosquito lipophorin had a hydrated density of 1.113 g / ml and contained 49 % lipid and 3.2 % carbohydrate . - pubmed
As model karyophiles we used digoxygenin labelled U1 snRNPs or fluorescein labelled U2 snRNPs . - pubmed
The role of bound lipid and transition metal in the formation of fluorescent advanced glycation endproducts by human serum albumin . - pubmed
In Group 1 mean serum levels of total cholesterol , low - density lipoprotein cholesterol and triglycerides were similar to those in mg / dl , p = 0.93 and 160 + /- 87 vs 155 + /- 76 , p = 0.74 , respectively ) while the mean serum level of high - density lipoprotein cholesterol was higher in Group 2 ( 49 + /- 4 vs 46 + /- 4 mg / dl , p = 0.005 ) . - pubmed
It was suggested that the down - regulation in PRL binding to some extent at least resulted from receptor overstimulation caused by the morphine - induced elevation in the concentrations of the endogenous hormone . - pubmed
The present study indicates that the relaxant effect of these compounds in intestinal smooth muscle is mediated through glibenclamide - sensitive ATP - dependent K + channels . - pubmed
We have monitored modifications of GK mRNA expression after different periods of time in culture and we have studied the effect induced by dexamethasone ( DEX ) treatment . - pubmed
DEX treatment inhibits GK mRNA expression in RIN cells in a dose - dependent and time - dependent manner . - pubmed
Striated muscle contraction is elicited by the release of stored calcium ions through ryanodine receptor channels in the sarcoplasmic reticulum . - pubmed
The rise in melanogenesis induced by NO - generating compounds is associated with an increased amount of both tyrosinase and tyrosinase - related protein 1 . - pubmed
Capillary electrophoresis ( CE ) with HeCd laser - induced fluorescence ( LIF ) detection and its application in forensic toxicology is demonstrated by the determination of D - lysergic acid diethylamide ( LSD ) in blood . - pubmed
Over 90 % frequently reported discussing the following topics with clients : home blood glucose monitoring , hyperglycemia , hypoglycemia , insulin use , and diet . - pubmed
We found that basal irIL-1 beta secretion is significantly inhibited by acetylcholine and histamine and increased by dopamine , while dexamethasone , IL-4 and IL-10 have no effect . - pubmed
We have studied the thermodynamics of binding of ( i ) mannitol to enzyme IImtl , ( ii ) the substrate analog perseitol to enzyme IImtl , ( iii ) perseitol to phosphorylated enzyme IImtl , and ( iv ) mannitol to enzyme IImtl treated with trypsin to eliminate the cytoplasmic domains . - pubmed
Sequence comparisons reveal a new amino acid sequence motif ( CP ) , conserved among the ciliated protozoan TERTs , and allow refinement of previously identified motifs . - pubmed
Examination of the components of Exosurf revealed that two compounds , DPPC and tyloxapol , showed inhibitory effects on CAT expression . - pubmed
The p53 binding and transactivation of the PTGF - beta promoter was enhanced by etoposide , a p53 activator , and was largely blocked by a dominant negative p53 mutant . - pubmed
Furthermore , expression of endogenous PTGF - beta was remarkably induced by etoposide in p53 - positive , but not in p53 - negative , cell lines . - pubmed
Cyclic AMP - specific phosphodiesterase and succinate dehydrogenase activities were suppressed only with 5 micrograms dose , but with rising dose levels the effect was not so apparent . - pubmed
Although the rise in JH titers and biosynthesis and the fall in JH esterase is correlated with ovarian development , application of methoprene or JH III in the absence of a carcass did not result in vitellogenin uptake by the oocytes . - pubmed
Although the role of kainate receptors on sensory afferents is unknown , it has been hypothesized that presynaptic kainate receptors may regulate glutamate release in the spinal cord . - pubmed
Here we show that kainate , applied at low micromolar concentrations in the presence of the AMPA - selective antagonist ( RS )- 4 -( 4 - aminophenyl )- 1 , 2 - dihydro-1 - methyl-2 - propyl - carbamoyl-6,7 - methylenedioxyphthalazine ++ + , suppressed spontaneous NMDA receptor - mediated EPSCs in cultures of spinal dorsal horn neurons . - pubmed
Interestingly , although the glutamate receptor subunit 5 - selective kainate receptor agonist ( RS )- 2 - alpha - amino-3 -( 3 - hydroxy-5 - tert - butylisoxazol-4 - yl ) propanoic acid ( ATPA ) ( 2 micrometer ) was able to suppress DRG - dorsal horn synaptic transmission to a similar extent as kainate ( 10 micrometer ) , it had no effect on excitatory transmission between dorsal horn neurons . - pubmed
Whereas DRG cell kainate receptors were sensitive to both kainate and ATPA , most dorsal horn neurons responded only to kainate . - pubmed
Finally , in recordings from dorsal horn neurons in spinal slices , kainate and ATPA were able to suppress NMDA and AMPA receptor - mediated EPSCs evoked by dorsal root fiber stimulation . - pubmed
Together , these data suggest that kainate receptor agonists , acting at a presynaptic locus , can reduce glutamate release from primary afferent sensory synapses . - pubmed
Asn49 is located in the type I beta turn formed by residues 49 - 52 , and is involved in extensive hydrogen - bonding interactions between alpha helix 2 and the rest of the N - terminal domain . - pubmed
In addition , the mutated enzyme exhibits : ( a ) an increase in the Km(GSH ) value of about 6.5 - fold , and decrease in kcat value of about fourfold ; ( b ) viscosity - independent kinetic parameters ; ( c ) lower thermostability , and ( d ) increased susceptibility to proteolytic attack by trypsin , when compared to the wild - type enzyme . - pubmed
Parasitologic ( xenodiagnosis , hemoculture , or both ) , histopathologic ( hematoxylin and eosin , and peroxidase - anti - peroxidase staining ) , and molecular ( polymerase chain reaction [ PCR ] followed by slot - blot hybridization ) tests were used in the analysis . - pubmed
Heme oxygenase and nitric oxide synthase are the enzyme systems responsible for generating carbon monoxide and nitric oxide , respectively . - pubmed
Both carbon monoxide and nitric oxide share similar properties , such as the ability to activate soluble guanylate cyclase to increase cyclic GMP . - pubmed
Although much is known about the heme oxygenase / carbon monoxide and nitric oxide synthase / nitric oxide pathways , how these two important systems interact is less well understood . - pubmed
We report the identification of a Ca ( 2 +)-responsive E - box element , CaRE2 , within BDNF promoter III that binds upstream stimulatory factors 1 and 2 ( USF1 / 2 ) and show that USFs are required for the activation of CaRE2 - dependent transcription from BDNF promoter III . - pubmed
We find that the transcriptional activity of the USFs is regulated by Ca ( 2 +)-activated signaling pathways in neurons and that the USFs bind to the promoters of a number of neuronal activity - regulated genes in vivo . - pubmed
Transcription factors , guidance molecules , extracellular matrix proteins , neurotrophic factors , and cell death - regulating factors of the Bcl-2 family and caspases , have all been reported to be involved in the processes of formation of a precise retino - collicular map , and regulation of developmental cell death . - pubmed
Testosterone binding to bovine serum albumin : effect of pH. - pubmed
THE RELATION OF GAS - CHROMATOGRAPHIC RETENTION TIME TO THE STRUCTURE OF STEROIDS AND DERIVATIVES . - pubmed
To test the stability of S2 , we constructed a series of " zippered " dimeric smooth muscle myosin II compounds , containing a high - melting temperature 32 - amino acid GCN4 leucine zipper in the S2 rod beginning 0 , 1 , 2 , or 15 heptads from the head - rod junction . - pubmed
We then assessed the ability of these and wild - type myosin to bind strongly via two heads to an actin filament by measuring the fluorescence quenching of pyrene - labeled actin induced by myosin binding . - pubmed
We find that wild - type and zippered heavy meromyosin ( HMM ) are able to bind by both heads to actin under both nucleotide - free and saturating ADP conditions . - pubmed
The proximate cause of the prepubertal quiescence in this neuroendocrine axis is the arrest or restraint of the pulsatile mode of hypothalamic GnRH release by a neurobiological brake that holds in check release of this decapeptide , without seeming to down - regulate the transcriptional activity of the gene encoding GnRH ( GnRH - I ) . - pubmed
The genetic and physiological factors ( with a particular emphasis on leptin ) that time the application and duration of the prepubertal brake on GnRH release are also considered . - pubmed
There was a direct correlation between BM and spleen uptake of FDG with increasing WBC , which was stronger than the inverse correlation seen with Hb ( the lower the Hb the greater the uptake ) . - pubmed
CONCLUSION : There is a correlation between hematologic parameters such as Hb , WBC and platelet counts and the uptake of FDG in BM and spleen in PET imaging . - pubmed
The expression of HIF-1alpha , endothelin-1 , inducible nitric oxide synthase , and vascular endothelial growth factor mRNA was highest during the active stage of ulcer healing , and endothelin-1 , vascular endothelial growth factor protein levels and nitric oxide were higher during the healing stage . - pubmed
Large scale molecular dynamics simulation of native and mutant dihydropteroate synthase - sulphanilamide complexes suggests the molecular basis for dihydropteroate synthase drug resistance . - pubmed
Dihydropteroate synthase ( DHPS ) has been targeted by sulphonamide inhibitors for the past 60 years and has developed different amino acid mutations to survive sulpha drug action . - pubmed
The amino acid residues serine at position 213 ( S213 ) and lysine at position 218 ( K218 ) , which are present in close proximity to the histidine - rich motif II of Mucor rouxii fatty acid Delta ( 6 )- desaturase isoform II , were targeted for studying structure - function relationships using site - directed mutagenesis . - pubmed
rouxii Delta ( 6 )- desaturase therefore has potential in specifically altering substrate utilization for production of desired fatty acids . - pubmed
In the repeated batch fermentations of the shake flasks , an optimum level of enzyme was maintained for 9 days using calcium alginate immobilized cells . - pubmed
Forskolin - induced cAMP formation was inhibited by [ D - Ala(2 ) , N - Me - Phe(4 ) , Gly ( 5 )- ol ]- enkephalin ( DAMGO 1 microM ) , a specific MOR agonist . - pubmed
Annexin V , RGS4 and CREB genes showed pronounced increase in expression after stimulation with DAMGO . - pubmed
Quantitative RT - PCR confirmed that DAMGO increased mRNA levels of Annexin V , an apoptosis - induced gene . - pubmed
Sera were assayed for progesterone ( P4 ) , prostaglandin F2alpha metabolite ( PGFM ) , and luteinizing hormone ( LH ) concentrations . - pubmed
UFAs homeostasis in many organisms is achieved by feedback regulation of fatty acid desaturase gene transcription through signalling pathways that are governed by sensors embedded in cellular membranes . - pubmed
During stimulation phospho - JNK levels were significantly increased at 3 , 6 , and 12 h after 0.1 or 0.5 microM arsenite exposure . - pubmed
Phospho - ERK1/2 levels were increased with different concentrations ( 0.1 - 10 microM ) of arsenite at 6 , 12 , and 24 h. Blocking of JNK pathway with 20 microM SP600125 or ERK1/2 by 100 microM PD98059 significantly inhibited biphasic effect of arsenite in cells . - pubmed
As for the pneumococcal infection in patient with good immunological response , good clinical effect might be obtained by panipenem / betamipron ( PAPM / BP ) 500 mg , imipenem / cilastatin ( IPM / CS ) 500 mg , meropenem ( MEPM ) 500 mg and biapenem ( BIPM ) 300 mg , b.i.d . - pubmed
, or IPM / CS 500 mg , MEPM 500 mg , t.i.d . - pubmed
At the bases of glands , intrinsic factor immunoreactive cells also were costained for 5 - bromo-2 '- deoxyuridine , suggesting their re - entry into the cell cycle . - pubmed
Inspectors from a public health center treating the housewife 's complaint quantified formaldehyde ( FA ) in high concentration exceeding Japanese national guideline of FA in some rooms of the house . - pubmed
The PETRA cross - linked polymers showed the best recognition ( MIP 2- , alpha=1.71 ) and selectivity ( MIP 2 + , alpha'=5.58 ) capabilities . - pubmed
Recently , it has been shown that TNF -[ alpha ] may be a novel target for the treatment of acute pancreatitis ; but the role of thalidomide , an immunomodulatory agent that inhibits TNF -( alpha ) and angiogenesis , has not been investigated so far . - pubmed
The study examined the cross - sectional relationship between carbohydrate , GI , GL and risk of the metabolic syndrome . - pubmed
Dietary carbohydrate , GI and GL were determined by an interview - administered FFQ . - pubmed
The risk of developing the metabolic syndrome was positively related to dietary carbohydrate ( P for trend = 0.03 ) , GI ( P for trend = 0.03 ) and GL intakes ( P for trend = 0.02 ) in women after adjusting for potential confounding variables . - pubmed
Among the components of developing the metabolic syndrome , the risk of high TAG and low HDL - cholesterol were positively related to high GI and GL intakes in women . - pubmed
The risk of developing the metabolic syndrome was considerably higher in the highest quintiles of carbohydrate ( OR 6.44 ; 95 % CI 2.16 , 19.2 ) , GI ( OR 10.4 ; 95 % CI 3.24 , 33.3 ) and GL intakes ( OR 6.68 ; 95 % CI 2.30 , 19.4 ) than in the lowest quintiles among women with a BMI & gt;/= 25 kg / m2 . - pubmed
However , there was no difference in risk across quintiles of carbohydrate , GI and GL among women with a BMI & lt ; 25 kg / m2 . - pubmed
Acetylcysteine therapy for chronic hepatitis C : are its effects synergistic with interferon alpha ? - pubmed
Group A was treated with lymphoblastoid IFNalpha 6MU three times a week for 6 months ; group B received the same schedule of lymphoblastoid IFNalpha as group A plus acetylcysteine 1200 mg / day per os in two administrations , and group C received only acetylcysteine 1200 mg / day per os in two administrations . - pubmed
Hypercatabolic syndrome ( HS ) is a biochemical state characterized by increased circulating catabolic hormones ( eg , cortisol , catecholamines ) and inflammatory cytokines ( eg , tumor necrosis factors , interleukin-1beta ) , and decreased anabolic insulin effects with consequent insulin resistance . - pubmed
The local ejection of glutamate over RS cells elicited excitatory responses that were only slightly depressed by 5 - HT . - pubmed
Second , in experiments with animals , it was found that SPS reduced serum triacylglycerol ( TG ) elevation induced by high - fat diets . - pubmed
SMN2 contains four CpG islands which present highly conserved methylation patterns and little interindividual variations in SMN1 - deleted SMA patients . - pubmed
The comprehensive analysis of SMN2 methylation in patients suffering from severe versus mild SMA carrying identical SMN2 copy numbers revealed a correlation of CpG methylation at the positions -290 and -296 with the disease severity and the activity of the first transcriptional start site of SMN2 at position -296 . - pubmed
We identified histone deacetylase ( HDAC ) inhibitors including vorinostat and romidepsin which are able to bypass SMN2 gene silencing by DNA methylation , while others such as valproic acid and phenylbutyrate do not , due to HDAC isoenzyme specificities . - pubmed
The phosphoprotein - bound beads suspended in a sample - loading dye solution were subjected to sodium dodecyl sulfate - polyacrylamide gel electrophoresis , followed by Western blotting . - pubmed
Remission was induced with prednisolone and intravenous cyclophosphamide , followed by maintenance therapy with azathioprine , during which MPO - ANCA results became negative . - pubmed
RESULTS : A panel of genes , proteins and metabolites , including PGE2 and TNF - alpha , were identified that describe a diclofenac - response network ( 68 genes in PBMC , 1 plasma protein and 4 plasma metabolites ) . - pubmed
Novel candidate markers of inflammatory modulation included PBMC expression of annexin A1 and caspase 8 , and the arachidonic acid metabolite 5,6 - DHET . - pubmed
However , the specific method of measuring calcium in serum using CPZ - III has not been established because of the difficulty of avoiding the interaction between CPZ - III and albumin . - pubmed
METHODS : In this study , we found that the non - specific reaction between CPZ - III and albumin could be controlled and calcium in serum could be specifically detected using CPZ - III combined with vanadate . - pubmed
The assay results correlated well with those of the Arsenazo - III ( 2,7 - bis ( 2 - arsonophenylazo )- 1,8 - dihydroxy-3,6 - naphthalenedisulphonic acid ) method ( slope = 1.067 ; intercept = -0.120 ; r = 0.989 ; Sy / x = 0.036 mmol / L ) , o - cresolphthalein complexone method ( slope = 0.911 ; intercept = 0.186 ; r = 0.988 ; Sy / x = 0.035 mmol / L ) , amylase enzymatic method ( slope = 0.981 ; intercept = 0.072 ; r = 0.989 ; Sy / x = 0.036 mmol / L ) and inductively coupled plasma emission spectroscopy method ( slope = 0.955 ; intercept = -0.001 ; r = 0.979 ; Sy / x = 0.048 mmol / L ) . - pubmed
The Her2 / neu receptor sites were labelled by immunofluorescence using conventional fluorescent dyes ( Alexa conjugated antibodies ) . - pubmed
In a preliminary batch adsorption experiment , H4 was evaluated to be highly efficient in the removal Hg ( II ) from synthetic aqueous solution . - pubmed
Drain blocking led to increases in the E4 : E6 ratio , and declines in specific absorbance , suggesting that DOC released from blocked drains consisted of lighter , less humic and less decomposed carbon . - pubmed
Whilst concentrations of DOC showed slight increases in drains and streams after blocking , instantaneous yields of both DOC and POC declined markedly in streams over the first year post - restoration . - pubmed
MAJOR CONCLUSIONS : Inositol is a building block of phosphatidylinositol ( PI ) , which is further elaborated to become PIMs , LM and LAM . - pubmed
Several additional control mechanisms must exist to control the complex biosynthetic pathways of PI , PIMs , LM and LAM . - pubmed
GENERAL SIGNIFICANCE : Mycobacteria have evolved a complex network of inositol metabolism . - pubmed
Diagnostic value of progesterone level and progesterone / estradiol ratio on the day of human chorionic gonadotropin administration . - pubmed
Our data indicate that resveratrol modulates the effect of MED28 on cellular migration , presumably through the EGFR / phosphatidylinositol 3 - kinase ( PI3 K ) signaling pathway , in breast cancer cells . - pubmed
At2g34630 is the main -- if not sole -- contributor to solanesyl diphosphate synthase activity required for the biosynthesis of ubiquinone , as demonstrated by the dramatic ( 75 - 80 % ) reduction of the ubiquinone pool size in corresponding RNAi lines . - pubmed
Overexpression of At2g34630 gave up to a 40 % increase in ubiquinone content compared to wild - type plants . - pubmed
AIM : Coronary angiography is usually done with heparin . - pubmed
With the increasing of PEHD concentration , the expression of caspase-8 ,- 9 ,- 3 and PARP protein increased . - pubmed
In this work , we present a combined scanning tunneling microscopy , secondary ions mass spectrometry , and magnetotransport study to understand the atomistic doping process of Ge by P2 molecules . - pubmed
Harnessing the one - dimer footprint of P2 molecules on the Ge(001 ) surface , we achieved the incorporation of a full P monolayer in Ge using a relatively low process temperature . - pubmed
JDS - chromium - insulin ( CRI)-003 is a novel form of insulin that has been directly conjugated with chromium ( Cr ) instead of zinc . - pubmed
Plasma glucose and insulin were measured . - pubmed
Insulin tolerance tests at week 7 showed that both insulin treatments significantly reduced glucose concentrations and increased insulin levels compared with the Sham group , CRI significantly reduced glucose at 4 and 6 h relative to Reg - In ( P & lt ; 0.05 ) , suggesting the effects of CRI on reducing glucose last longer than Reg - In . - pubmed
cholerae resistance against bile salts , perhaps due to environmental regulation of AmiB or its activators . - pubmed
Efficacy and safety of nadifloxacin for bacterial skin infections : results from clinical and post - marketing studies . - pubmed
METHODS : This article presents the results of one post - marketing surveillance ( PMS ) and three randomized , open , non - blinded , multi - centric clinical studies that compared nadifloxacin with mupirocin and framycetin , and nadifloxacin with fusidic acid . - pubmed
Intracellular Ca ( 2 + ) level was detected by Hitachi - 850 fluorospectrophotometer , calcineurin ( CaN ) activity was measured by colorimetric method , the expression of CaN protein was observed with immunocytochemistry , and the proto - oncogene c - fos mRNA expression was assessed with reverse transcription polymerase chain reaction ( RT - PCR ) . - pubmed
However , actin assembly , spindle structure and chromosome alignment were not disrupted after exposure to 50 muM AFB1 . - pubmed
AFB1 treatment ( 50 muM ) also induced oxidative stress and further led to autophagy , as shown by accumulation of reactive oxygen species , up - regulated LC3 protein expression and increased mRNA levels of ATG3 , ATG5 and ATG7 . - pubmed
Annexin V - FITC staining assay revealed that AFB1 treatment ( 50 muM ) resulted in oocyte early apoptosis , which was confirmed by increased Bak , Bax , Bcl - xl mRNA levels . - pubmed
In addition , TMC reduced protein expression of Akt , MMP-2 and MMP-9 and enhanced E - cadherin and TIMP-1 protein expression , which are known to regulate cell adhesion , migration and invasion . - pubmed
Here , we combine fluorescence correlation spectroscopy and acrylodan fluorescence screening to study the stoichiometry and structural features of tau - tubulin assemblies . - pubmed
The DLS , TEM , AFM , FT - IR and Powder - XRD studies demonstrate strong binding of creatinine and urea to the nanoparticles . - pubmed
Real - time PCR was applied to determine any variation in levels of expression of the serotonin ( Slc6A4 or Sert ) and dopamine transporter ( Slc6A3 or Dat ) genes . - pubmed
In order to better understand the regulation and role of NF - kappaB in acute kidney injury we explored the expression of NF - kappaB - related genes in experimental acute kidney injury induced by a folic acid overdose . - pubmed
Kidney NFkappaBiz mRNA upregulation and protein downregulation was also observed in acute kidney injury induced by cisplatin or unilateral kidney injury resulting from ureteral obstruction . - pubmed
FAA was also positively related to the level of restriction and inversely to leptin , insulin , TNF - alpha and IGF-1 . - pubmed
The Secreted Enzyme PM20D1 Regulates Lipidated Amino Acid Uncouplers of Mitochondria . - pubmed
We demonstrate that PM20D1 is a bidirectional enzyme in vitro , catalyzing both the condensation of fatty acids and amino acids to generate N - acyl amino acids and also the reverse hydrolytic reaction . - pubmed
N - acyl amino acids directly bind mitochondria and function as endogenous uncouplers of UCP1 - independent respiration . - pubmed
Detection of reactive oxygen species ( ROS ) and measurement of mitochondrial membrane potential ( MMP ) were performed by flowcytometry . - pubmed
Fasting blood glucose , insulin and HBA1C level and different oxidative and anti - oxidative stress parameters were measured and pancreatic tissue sections were stained with eosin - haematoxylene . - pubmed
Nicotine also caused oxidative stress in pancreatic tissue as evidenced by increased nitric oxide and malondialdehyde level and decreased superoxide dismutase , catalase and reduced glutathione level . - pubmed
Despite its proven benefits , half of the patients treated with imatinib show disease progression within 2 years due to secondary resistance mutations in KIT . - pubmed
Pre - existing cancer cells with genetic alterations promoting apoptosis resistance may serve as a basis whereby cancer cells with critical mutations , such as secondary KIT mutations , can establish full imatinib resistance . - pubmed
Antral follicle count ( AFC ) ( p = 0.001 ) , total FSH stimulating dose ( p = 0.0001 ) , and peak serum estradiol level ( p = 0.0001 ) were associated with spindle angle grouping . - pubmed
According to the ratio of GAPDH / CS and the LDH level the gasterozooids prefer the anaerobic glycolytic pathway whereas in the sexual hydranths relatively more substrate is supplied at the disposal of the citrate cycle . - pubmed
Significant sequential reductions in P2Y12 reaction units were noted , in the following order : clopidogrel , low - dose ticagrelor , and standard - dose ticagrelor ( ANCOVA , p<0.001 ) . - pubmed
After PSA ( antigen , Ag ) was added into the system , a noncompetitive immune reaction was happen between Ab * and Ag to form an immune complex ( Ag - Ab * ) . - pubmed
We hypothesized that beta - AR blockade with propranolol would restore endothelial function and vascular insulin signaling in obesity , associated with an anti - inflammatory effect . - pubmed
Protective effect of propranolol administration in endothelial function was associated with increased nitric oxide ( NO ) production and phosphorylation of Akt ( Ser473 ) and eNOS ( Ser1177 ) , but with reduced phospho - IRS-1(Ser307 ) and phospho - ERK1 / 2 ( Thr202 / Tyr204 ) . - pubmed
In conclusion , our results suggest that beta - AR blockade with propranolol is effective to prevent the endothelial dysfunction , vascular IR and pro - inflammatory state displayed in HFD - induced obesity , independent of changes in body weight . - pubmed
We studied the growth of Ge layers on Au ( 111 ) under ultra - high vacuum conditions from the submonolayer regime up to a few layers with Scanning Tunneling Microscopy ( STM ) , Direct Recoiling Spectroscopy ( DRS ) and Low Energy Electron Diffraction ( LEED ) . - pubmed
The high surface sensitivity of TOF - DRS allows us to confirm the coexistence of Au and Ge atoms in the top layer for all stages of growth . - pubmed
Here , asialoglycoprotein receptor ( ASGP - R)-targeting paclitaxel - conjugated gold nanoparticles ( Gal / PTX - GNPs ) are fabricated as a demonstration to realize the precise treatment of liver cancer . - pubmed
The drug level in tumor versus liver of Gal / PTX - GNPs is 121.0 % at 8 h post injection , a 15.7 - fold increase in the tumor specificity compared to that of GNPs conjugated with PTX only . - pubmed
Intra - cerebral cannabidiol infusion - induced neuroprotection is partly associated with the TNF - alpha / TNFR1 / NF - kB pathway in transient focal cerebral ischaemia . - pubmed
Down - regulation of TNF - alpha , TNFR1 , and NF - kB expression was also observed by CBD . - pubmed
CONCLUSION : The results achieved in this study support the idea that CBD has a cerebroprotective effect ( partly through suppression of TNF - alpha , TNFR1 , and NF - kB ) on ischaemic injury . - pubmed
Non - fasting total cholesterol , triglycerides and HDL - Cholesterol ( HDL - C ) were measured . - pubmed
25 ( OH ) D is inversely correlated with triglycerides and non - HDL - C in the total population and in boys and girls ( p & lt ; 0.0001 for all ) , and positively correlated with HDL - C in the total population and in boys but not in girls ( p = 0.001 , p & lt ; 0.0001 and p = 0.1 , respectively ) . - pubmed
In a multilinear regression analysis , in the overall population , 25 ( OH ) D is independently associated with sex , season , school 's SES , BMI , triglycerides and non - HDL - C. In boys , the association with BMI and season is non - significant . - pubmed
We demonstrated that lysine 830 of BRCA1 is a preferential acetylation site by pCAF and tested its function in embryonic stem ( ES ) cells by changing lysine 830 to arginine using a transcription activator - like effector nuclease ( TALEN ) system . - pubmed
These data suggest that acetylation of BRCA1 on lysine 830 activates BRCA1 function at the intra - S checkpoint after DNA damage . - pubmed
Analysis of mercury adsorption at the gibbsite - water interface using the CD - MUSIC model . - pubmed
In the present study , simulation experiments on macroscopic Hg ( II ) sorption by gibbsite ( alpha - Al ( OH ) 3 ) , a representative aluminum ( hydr ) oxide mineral , were performed using the charge distribution and multi - site complexation ( CD - MUSIC ) approach with 1 - pK triple plane model ( TPM ) . - pubmed
The model successfully incorporated the presence of inorganic ligands at the dominant edge ( 100 ) face of gibbsite with consistent surface species , which was evidenced by molecular scale analysis . - pubmed
Annexin V / propidium iodide and terminal deoxynucleotidyl transferase - mediated dUTP nick end labeling assay was used to detect BLE - induced apoptosis . - pubmed
CONCLUSIONS : : BLE induced NPDF apoptosis and ECM degradation through a mitochondrial - mediated pathway and in a ROS - dependent manner . - pubmed
Here , we determined whether chronic intermittent access ( CIA ) to alcohol during adolescence / early adulthood or during adulthood would alter or be associated with auditory - cued conditioned fear levels using an extended training fear incubation procedure . - pubmed
Interventions of interest are open surgery ( ie , saphenofemoral or high ligation ( HL ) with stripping ) and endovenous ablations ( ie , EVLA , RFA , EVSA , MOCA , cyanoacrylate injection and UGFS ) . - pubmed
TH did not confer protection against flurothyl seizure susceptibility at P18 in this model . - pubmed
The ensemble networks enabled accurate predictions for full - thickness reference properties ( PG content : rhoin vitro , Val= 0.691 , rhoin vivo= 0.676 ; collagen orientation angle : rhoin vitro , Val= 0.626 , rhoin vivo= 0.574 ) from NIR spectral data . - pubmed
UPC2A deletion also increased susceptibility to both triazoles and tetrazoles , but to a lesser extent than PDR1 deletion . - pubmed
To further investigate the mechanism by which DNA - PKcs promotes cSCC progression , TGF - beta1 and the TGF - beta receptor ( TGF - betaR ) I / II dual inhibitor LY2109761 were used to examine whether DNA - PKcs participates in TGF - beta1 / Smad signaling . - pubmed
Background : Methylenetetrahydrofolate reductase ( MTHFR ) has a key role in converting homocysteine to methionine so its deficiency might cause homocysteine accumulation and arterial destruction . - pubmed
Serum homocysteine and MDA level as well as MTHFR activity were measured manually . - pubmed
Also , the results indicate that MTHFR activity in the two groups was statistically different ( p - value < 0.001).Conclusion : It can be concluded that the regulation of homocysteine and its metabolic enzymes plays a significant role in controlling blood pressure and consequently in the pathophysiology of Preeclampsia and it can be a goal for prevention and treatments . - pubmed
Suberoylanilide hydroxamic acid ( SAHA ) , a clinically used HDAC inhibitor , was then intraperitoneally and intrathecally injected to rescue the increased expression levels of HDAC1 and HDAC2 . - pubmed
RESULTS : TCI caused rapid and long - lasting increased expression of HDAC1 / HDAC2 in glial cells of the spinal dorsal horn and dorsal root ganglia . - pubmed
Inhibiting HDACs by SAHA not only reversed TCI - induced upregulation of HDACs but also inhibited the activation of glial cells in the spinal dorsal horn and dorsal root ganglia , and relieved TCI - induced mechanical allodynia . - pubmed
Moreover , we found that SAHA administration could inhibit GSK3beta activity in the spinal dorsal horn and dorsal root ganglia , which might contributed to the relief of BCP . - pubmed
CONCLUSION : Our findings suggest that HDAC1 and HDAC2 are involved in the glia - mediated neuroinflammation in the spinal dorsal horn and dorsal root ganglia underlying the pathogenesis of BCP , which indicated that inhibiting HDACs by SAHA might be a potential strategy for pain relief of BCP . - pubmed
The Role of Ca2 + -NFATc1 Signaling and Its Modulation on Osteoclastogenesis . - pubmed
Cytosolic calcium binds to calmodulin and subsequently activates calcineurin , leading to NFATc1 activation , a master transcription factor required for osteoclast differentiation . - pubmed
Thus , in this review , we summarized the modulation of the NFATc1 pathway through various compounds that regulate calcium signaling and the calcium influx machinery . - pubmed
Amorphous and crystalline dihydrate formulations showed no change in aerosolization parameters ( emitted dose and FPF ) upon storage for three months . - pubmed
Following aerosolization , islet cell vitality and function were assessed via Calcein AM and propidium iodide as well as insulin production after glucose exposure . - pubmed
, gpx1b , gpx4a , gpx7 and sod3a ) involved in detoxifying cellular reactive oxygen species were downregulated significantly . - pubmed
Among non - fermenting Gram - negative bacilli , fluoroquinolone resistance is mostly due to amino acid substitutions in localized regions of the targets ( GyrA , GyrB , ParC and ParE ) named QRDRs , but also to efflux . - pubmed
METHODS : The putative QRDRs of GyrA , GyrB , ParC and ParE were sequenced in 62 clinical isolates , and in vitro one - step mutants obtained after exposure to fluoroquinolones . - pubmed
RESULTS : We detected seven substitutions in QRDRs ( Q83L / S84P / D87N / D87 G for GyrA , Q480P for GyrB , T395A / K525Q for ParE ) , all in nine of the 27 clinical isolates with ciprofloxacin MIC > = 16 mg / L , whereas none among the in vitro mutants . - pubmed
Substitutions in AxyT associated with overexpression of AxyEF - OprN were also detected in seven clinical strains with ciprofloxacin MIC > = 16 mg / L. CONCLUSIONS : Target alteration is not the primary mechanism involved in fluoroquinolone resistance in Achromobacter . - pubmed
BACKGROUND : Infections caused by Enterobacteriaceae are mainly treated with the beta - lactam antibiotics , nevertheless , the emergence of species with plasmid - borne beta - lactamases has decreased the efficacy of these antibiotics . - pubmed
Among 35 cefoxitin resistant isolates , 18 ( 51.4 % ) identified as carrying AmpC genes by multiplex PCR . - pubmed
Within AmpC beta - lactamase genes , DHA gene was the predominant gene ( 15 , 42.3 % ) . - pubmed
Conclusions : Administration of insulin plus metformin in type-1 diabetes could modulate the function of CYP2D1 to the observed levels in the control group and made it clearer to predict the fate of drugs that are metabolized by this enzyme . - pubmed
Methods : Amorphous zirconia was deposited using an atomic layer deposition reactor ( Atomic Classic , CN1 , Hwaseong , Korea ) on titanium discs . - pubmed
The purified protease yielded only 1 line in the SDS - polyacrylamidegel - electrophoresis , in the gelatin - polyacrylamidegel - electrophoresis and in the double immuno - diffusion test ( fig . - pubmed
The protease - activity was completely inhibited in the presence of EDTA , partially inhibited by Cu2 + and Zn2 + and increased by Mn2 + ( table 3 ) . - pubmed
The actions of neuroleptic drugs and putative serotonin receptor antagonists on LSD and quipazine - induced reductions of brain 5 - HIAA concentrations . - pubmed
The formation of the unusual species of lecithin in tumor tissues was discussed in relation to the impairing in the enzyme systems involved in lecithin synthesis . - pubmed
[ Serum glucose determination using the hexokinase and glucose dehydrogenase technic on the autoanalyzer ] . - pubmed
The prevalence of hepatitis - B antigen ( HB S Ag ) and its antibody ( Anti - HB S ) among London hemophiliacs and blood donors from London and two tropical areas . - pubmed
The prevalence of hepatitis - B antigen ( HB s AG ) and its antibody ( anti - HBs ) among 49 London hemophiliacs was compared with that among women attending an antenatal clinic and healthy blood donors from London and from two parts of the tropics ( Mauritius and Zambia ) . - pubmed
Calcium channels and nifedipine inhibition of serotonin - induced [ 3H ] thymidine incorporation in cultured cerebral smooth muscle cells . - pubmed
Passaged cultures ( 10 - 30 passages ) that expressed serotonin receptors were studied using [ 3H ] thymidine incorporation . - pubmed
When tested in quiescent medium , serotonin potently stimulated [ 3H ] thymidine incorporation ( EC50 of 31 nM ) by as much as 400 % at 24 h. The number of cells was not significantly increased at 24 or 48 h. At concentrations of 10(-8)-10(-5 ) M 5 - HT , [ 3H ] thymidine uptake was reduced 40 - 50 % by the dihydropyridine Ca2 + channel blocker , nifedipine ( 1 microM ) . - pubmed
To demonstrate a possible mechanism for the sensitivity to nifedipine , Ca2 + currents were measured using the whole cell patch clamp technique . - pubmed
The cells expressed dihydropyridine - sensitive L - type Ca2 + channels , but not other subtypes of Ca2 + channels , as indicated by the kinetic and voltage - dependent characteristics of the current and by the stimulatory effect of Bay K 8644 . - pubmed
Significant reductions of estradiol ( p less than 0.01 ) and CA125 ( p less than 0.001 ) levels after three courses of chemotherapy were observed . - pubmed
In experiments on agonist - induced PA , TMS inhibited PA and ATP secretion 5 - fold more strongly than DMS or SPN . - pubmed
Tumor cell ( BL6 melanoma)-induced PA and ATP secretion were also strongly inhibited by TMS , but not by DMS or SPN . - pubmed
Unlike ADP- or thrombin - induced PA , BL6 cell - induced PA was not inhibited by Calphostin - C ( a potent protein kinase C inhibitor ) or cilostazol ( a potent inhibitor of PA based on inhibition of cyclic AMP phosphodiesterase ) . - pubmed
The nucleotide sequence of the 5 ' portion of this gene revealed a coding sequence for a 60 residue propeptide containing two Lys - Arg amino acid pairs that have been identified as sites for peptidase processing of several exported peptides and proteins . - pubmed
The final Lys - Arg site occurs at the junction with the mature extracellular form of the acid protease . - pubmed
Four 25 - nt long oligonucleotides containing dA and dT ( D1 , D2 , D3 , and D4 ) which are capable of forming parallel - stranded ( ps ) or antiparallel - stranded ( aps ) duplexes have been synthesized [ Rippe , K. , Ramsing , N. B. , & amp ; Jovin , T. M. ( 1989 ) Biochemistry 28 , 9536 - 9541 ] . - pubmed
A similar result was observed with KMnO4 and DEPC , although with the latter reagent the modification pattern of the labeled strands D1 * and D4 * was slightly different for the parallel than for the antiparallel duplex . - pubmed
It is possible , therefore , that the FAE generally performs class II - restricted transport and presentation to T cells of antigens which have been adequately processed in the gut lumen . - pubmed
Sphingomyelin was not accumulated , while lysosomal sphingomyelinase was dramatically inhibited . - pubmed
Sodium dodecyl sulfate - polyacrylamide gel electrophoresis and two - dimensional gel electrophoresis showed that the rod portion of the released structures consisted of the 30 - kilodalton FlgG protein . - pubmed
[ DNA - duplexes with phosphoamide bond : interaction with restriction endonucleases EcoRII and SsoII ] . - pubmed
We studied the interaction of EcoRII and SsoII restriction endonucleases with synthetic DNA duplexes , containing 3'N----5'P and 3'P----5'N phosphoamide internucleotide bonds in one of the cleavage points . - pubmed
The presence of phosphoamide bonds was found to reduce the rate of cleavage of the natural strand by EcoRII and to have no influence in case of SsoII . - pubmed
In the presence of Mg2 + ions the equilibrium association constant of the enzyme - substrate complex is 3 - fold reduced , and the dissociation rate constant of the complex is increased by 1.5 times . - pubmed
Routine detection of IgG oligoclonal bands and free light chains patterns by means of agarose isoelectric focusing and avidin - biotin - peroxidase complex staining in multiple sclerosis unconcentrated cerebrospinal fluid . - pubmed
Administration of a thiol radioprotector beta - mercaptoethylamine resulted in alteration of kinetic properties of tryptophane hydroxylase expressed as a decrease in the optimal concentration of the substrate ; this decrease was more distinct in liver and small intestine as compared with mast cells . - pubmed
Isotherms of the adsorption of acridine dyes on tRNA . - pubmed
The results presented indicate that AMINO , like AMI , inhibits NA uptake and is a 5 - HT antagonist . - pubmed
When combining the carbohydrate detection method with the peroxidase -- antiperoxidase ( PAP ) method used for immunocytochemical detection of the various pancreatic islet hormones , paraffin sections reveal that glycogen is primarily localized in granulated B cells ; the degranulated B cells also contain glycogen , though in smaller amounts . - pubmed
In contrast , the islet cells containing somatostatin , glucagon and pancreatic polypeptide are nearly free of glycogen . - pubmed
[ Feasibility of a cyclophosphamide methotrexate and 5 - fluorouracil regime intravenously administered with and without granulocyte - colony stimulating factor in surgically treated breast carcinoma ] . - pubmed
The aim of this study was to evaluate the feasibility of CMF 1.8 - 28 regimen , with all three drugs administered intravenously ( IV ) , and to compare the hematologic toxicity of this regimen with or without G - CSF . - pubmed
Treatment with G - CSF after CMF 1.8 regimen resulted in a significantly WBC 's earlier nadir ( average day of nadir 14 vs 17 ; p = 0.0005 ) , while there was no difference in the average values of WBC at the nadir . - pubmed
CMF 1.8 with IV cyclophosphamide is a feasible regimen with and without G - CSF and can be used in adjuvant setting instead of " classic " CMF in order to improve the low compliance observed when cyclophosphamide is given by mouth . - pubmed
Sequencing of the FBN1 gene revealed a heterozygous C to T transition at nucleotide 8176 resulting in the substitution of a tryptophan for an arginine ( R2726W ) , at a site immediately adjacent to a consensus sequence recognized by a cellular protease . - pubmed
The positional distribution of the thioic acid ester in the lipid was determined by beta - galactosidase , sulphur - oxygen exchange reaction using silver nitrate , and lipase hydrolysis of the diacylglycerol derived from the lipid . - pubmed
Serum cortisol was determined several times a day before and just after hrec TNF alpha first immunotherapeutic cycle . - pubmed
The results of this study showed the decreasing influence of hrec TNF alpha administration on cortisol secretion by adrenals with no disturbances in that circadian rhythm . - pubmed
IN CONCLUSION : It might be possible that some side effects of hrec TNF alpha administration are connected with the decreasing cortisol level . - pubmed
Anti - lactoferrin autoantibodies : relation between epitopes and iron - binding domain . - pubmed
We hypothesized that the interaction of anti - LF antibodies with the LF iron binding domain might contribute to pathogenesis by inhibiting iron chelation after neutrophil activation , thereby providing increased iron availability for endothelial cell damage . - pubmed
Therefore , anti - LF autoantibodies did not appear to modulate LF iron - binding activity . - pubmed
Total and selenium - dependent glutathione peroxidase activities in heart were unaffected by any treatment . - pubmed
DHEA increased catalase activity at rest . - pubmed
Exercise increased catalase activity ( 71.5 + /- 7.9 vs. 97.4 + /- 9.5 mu mol x min-1 x mg protein-1 ) ; however , when VitE was given , the response to exercise was attenuated ( 74.1 + /- 8.4 vs. 80.9 + /- 9.9 mu mol center dot min-1 x mg protein-1 ) . - pubmed
The results show that neither mutation is likely to be the etiology for predisposition to a change of high - density lipoprotein cholesterol and/or variation in lipid and lipoprotein levels , or for the occurrence of coronary heart disease in Japanese populations . - pubmed
Vav , a GDP - GTP exchange factor for Rho - family proteins , is tyrosine phosphorylated following TCR stimulation , suggesting that it may transduce TCR signals . - pubmed
Vav may influence the efficiency of TCR - induced selection events by regulating the intracellular calcium flux of thymocytes . - pubmed
We demonstrated that the formation of prostaglandin E2 ( PGE2 ) is highly dependent on the expression of PGHS-2 enzyme in these cells . - pubmed
TNF - alpha - induced PGE2 production was evident after 12 h and was associated with a significant TNF - alpha - mediated increase in PGHS-2 immunoreactive protein . - pubmed
A specific PGHS-2 inhibitor , NS-398 , completely abolished the TNF - alpha - mediated increase in PGE2 production , suggesting that the PGE2 formed in response to TNF - alpha was derived from PGHS-2 . - pubmed
TNF - alpha - mediated PGHS-2 mRNA accumulation was observed at 1 h , remained elevated for 24 h , and was blocked by actinomycin D , indicating that TNF - alpha increases PGHS-2 gene transcription . - pubmed
All the strains were characterized by the low production of higher alcohols and the high production of ethyl acetate , whereas consistent differences in the production of acetaldehyde , acetoin and acetic acid served to differentiate star fruit apiculate strains into six different phenotypes , present at different stages of the fermentation process . - pubmed
Potent , non - thiol inhibitors of farnesyltransferase . - pubmed
The structure - activity relationship of a series of non - thiol CaaX analogs , which are inhibitors of farnesyltransferase , is described . - pubmed
These inhibitors contain a substituted phenyl group at the N terminus , which may occupy a novel binding domain on the Ras protein . - pubmed
CD44 is a ubiquitous molecule also known as hyaluronic acid or homing receptor . - pubmed
These results indicate that the adhesion of RA synovial cells to matrices such as hyaluronic acid through CD44 could up - regulate VCAM-1 expression and VCAM-1 - mediated adhesion to T cells , which might in turn cause activation of T cells and synovial cells in RA synovitis . - pubmed
We found that isoamyl alcohol induces cell extensions in pkc1 deletion mutants but not in mutants with mutations in genes positioned downstream or upstream of the PKC1 gene . - pubmed
Cytochalasin D ( 0.08 mg / mL ) inhibited the formation of actin cable but did not affect the induction of cell extensions . - pubmed
At the end of the treatment period with PGE1 , ET-1 plasma concentration decreased from 4.50+/-0.8 pmol / l to 3.6+/-1.1 pmol / l ( p = 0.002 ) , whereas it remained unchanged in placebo group . - pubmed
A significant correlation between the decrease in ET-1 plasma levels and the increase in the PFWD and MWD ( r = -0.92 , p & lt ; 0.0001 ; r = -0.78 , p = 0.002 , respectively ) was detected in PGE1 treatment group . - pubmed
Their stability constants are determined with a recent PC program SIRKO and the calculated values are : for cobalt , log beta 1 = 1.6 , log beta 2 = 2.7 ; for nickel , log beta 1 = 1.8 , log beta 2 = 3.0 ; and for copper , log beta 1 = 3.0 , log beta 2 = 3.6 . - pubmed
BACKGROUND AND AIM : The aim of the study was to evaluate the effect on blood glucose levels in non - insulin - dependent diabetics ( NIDDM ) of reduction of the carbohydrate content through the use of a new , almost starch - free type of bread ( SF - bread ) . - pubmed
Peak blood glucose concentration was 14.8 + /- 2.3 mM on the control day and 11.6 + /- 1.7 mM on the SF - bread day ( p & lt ; 0.001 ) . - pubmed
In the second part of the study , the diet including SF - bread reduced fasting blood glucose from 13.3 + /- 3.5 mM to 10.2 + /- 2.0 mM ( p & lt ; 0.006 ) and the fraction of HbA1c from 0.090 + /- 0.014 to 0.081 + /- 0.015 ( p & lt ; 0.02 ) . - pubmed
Serum cholesterol levels were significantly reduced by the SF - bread as compared to the ordinary diet ( 5.8 + /- 0.6 to 5.5 + /- 0.5 mM versus 5.7 + /- 0.8 to 5.8 + /- 0.7 mM ; p & lt ; 0.05 ) . - pubmed
The beneficial reduction in mortality with abciximab use in diabetics classified as insulin - requiring was from 8.1 % to 4.2 % , p = 0.073 . - pubmed
Syntheses and evaluation of halogenated cytisine derivatives and of bioisosteric thiocytisine as potent and selective nAChR ligands . - pubmed
The 3 - halocytisines 7 possess subnanomolar affinities for ( alpha 4)(2)(beta 2)(3 ) nAChRs , higher than those found for ( -)- cytisine as well as for the 5 - halocytisines 8 and 3,5 - dihalocytisines 6 . - pubmed
The most potent molecule was 3 - bromocytisine ( 7b ) with preferential selectivity ( 200 - fold ) for the ( alpha 4)(2)(beta 2)(3 ) subtype [ K(i)=10 pM ( alpha 4 beta 2 ) and 2.0 nM ( alpha 7 * ) ] . - pubmed
Replacement of the lactam with a thiolactam pharmacophore to thiocytisine ( 12 ) resulted in a subnanomolar affinity for the ( alpha 4 )( 2 )( beta 2 )( 3 ) nAChR subtype ( K(i)=0.832 nM ) , but in a drastic decrease of affinity for the alpha 7 * subtype ; thiocytisine ( 12 ) has a K(i ) value of 4000 nM ( alpha 7 * ) , giving a selectivity of 4800 - fold for the neuronal ( alpha 4 )( 2 )( beta 2 )( 3 )- nAChR and thus displaying the best affinity - selectivity profile in the series under consideration . - pubmed
Four immunosuppressive eras were analyzed : ( 1 ) the precyclosporine era , era 1 ( n = 65 ; 16 % ) ; ( 2 ) the cyclosporine era , era 2 ( n = 109 ; 27 % ) ; ( 3 ) the tacrolimus era with monoclonal or polyclonal antibody induction therapy , era 3 ( n = 104 ; 26 % ) ; and ( 4 ) the tacrolimus era with monoclonal and polyclonal antibody induction therapy , era 4 ( n = 128 ; 31 % ) . - pubmed
Noradrenergic transmission may also be implicated because most psychostimulants increase norepinephrine ( NE ) release , and numerous studies have indicated interactions between noradrenergic and dopaminergic neurons through alpha1 - adrenergic receptors . - pubmed
Similar to natural enzyme , recombinant xylitol dehydrogenase is a functional homotetramer with a stoichiometric content of catalytic zinc in each 37 - kDa subunit . - pubmed
Combined results of initial - rate analysis and isotope effect studies suggest that the enzyme utilizes a preferentially ordered kinetic mechanism in which NAD (+ ) binds before D - sorbitol and D - fructose is released before NADH . - pubmed
Stabilising mutations , which render Aux / IAA proteins resistant to auxin - mediated degradation , confer a wide range of phenotypes consistent with disruptions in auxin response . - pubmed
These results suggest a general model for auxin signalling in which the modulation of the relative abundance of different Aux / IAA proteins can determine which down - stream responses are induced . - pubmed
Follow - up with regular thyroglobulin assay and ( 131 ) I whole - body scan is capable of detecting residual or recurrent disease with great sensitivity and specificity . - pubmed
This article will review the current management of differentiated carcinoma of the thyroid and the possible causes of the reported inadequacy of thyroglobulin and ( 131 ) I whole - body scan to detect residual or recurrent disease , and the increasing role of alternative imaging , particularly ( 18 ) F - FDG PET in the management of this curable malignancy . - pubmed
Her prolactine and estradiol levels were increased but luteinizing hormone and follicle - stimulating hormone were decreased . - pubmed
The H2S - induced relaxation of MAB was partially mediated by ATP - sensitive K + ( KATP ) channel activity in vascular smooth muscle cells . - pubmed
DL - propargylglycine ( a blocker of CSE ) at 10 microM abolished L - cysteine - dependent increase in H2S production and relaxation of MAB . - pubmed
The stimulation of KATP channels in vascular smooth muscle cells and charybdotoxin / apamin - sensitive K + channels in vascular endothelium by H2S represents important cellular mechanisms for H2S effect on MAB . - pubmed
Using [ ( 125 ) I]alpha - bungarotoxin binding assays and voltage clamp , we determined that the nAChR expression , EC(50 ) , and Hill coefficient values for WT are 1.8 + /- 0.4 fmol , 30.3 + /- 1.1 microM , and 1.8 + /- 0.3 , respectively . - pubmed
The periodicity in function ( EC(50 ) and macroscopic peak current ) and nAChR expression reveals an average of 3.3 and 3.0 amino acids respectively , thus suggesting a helical secondary structure for the gammaTM4 . - pubmed
This element is a key moiety of several enzymes in iron containing heme or nonheme form and transfer and storage protein , hemoglobin and myoglobin . - pubmed
The redox state of iron is determined by xanthine oxidase , cytochromes and Hp or ceruloplasmin and ferroxidase activity of apo - ferritin , respectively . - pubmed
Some vitamins ( C , B2- , B3- , B6- , B12 ) play also a role in the metabolism of iron . - pubmed
We found that , together , solvent - exposed heme 1 ( having a large negative redox potential ) and heme 2 ( having a large positive redox potential ) form a lock for electrons generated during the oxidation reaction The attachment of HAO 's physiological electron transfer partner cytochrome c(554 ) results in a positive shift in the redox potential of heme 1 , and " opens the electron gate " . - pubmed
The closeness of hemes 3 and 8 from different enzyme subunits allows redistribution of the four electrons generated as a result of hydroxylamine oxidation , among the three enzyme subunits . - pubmed
Toxaphene congener profiles were relatively invariant throughout the watershed and were dominated by 2,2,5 - endo,6 - exo,8,8,9,10 - octachlorobornane ( P-42a or B8 - 806 ) with lesser amounts of several other Cl ( 7 )- Cl ( 9 ) compounds , many of which remain unidentified . - pubmed
B2Rs were coupled with Gq / 11 and their activation resulted in diacylglycerol and inositol triphosphate release . - pubmed
The mechanism for prostaglandin - induced augmentation of BK response is left open for future study . - pubmed
Expression of HOXB2 , a retinoic acid signaling target in pancreatic cancer and pancreatic intraepithelial neoplasia . - pubmed
RESULTS : We identified aberrant expression of several components of the retinoic acid ( RA ) signaling pathway ( RARalpha , MUC4 , Id-1 , MMP9 , uPAR , HB - EGF , HOXB6 , and HOXB2 ) , many of which are known to be aberrantly expressed in pancreatic cancer and PanIN . - pubmed
NUCLEAR QUADRUPOLE AND SPIN - ROTATION INTERACTION OF O IN FORMALDEHYDE . - pubmed
Phosphatidylinositol 3 - kinase - dependent activation of akt , an essential signal for hyperthermia - induced heat - shock protein 72 , is attenuated in streptozotocin - induced diabetic heart . - pubmed
We tested the hypothesis that phosphatidylinositol 3 - kinase ( PI 3 - kinase)-dependent activation of Akt is essential for the expression of cardiac heat - shock protein 72 ( HSP72 ) and that this pathway is impaired in the streptozotocin ( STZ)-induced diabetic heart . - pubmed
Compared with control heart , hyperthermia - induced HSP72 expression and phosphorylation of Akt were attenuated in the STZ - vehicle heart . - pubmed
Pretreatment with wortmannin attenuated hyperthermia - induced HSP72 expression and phosphorylation of Akt . - pubmed
Wortmannin and LY294002 inhibited hyperthermia - induced HSP72 expression and phosphorylation of Akt . - pubmed
Our results indicate that activation of Akt , in a PI 3 - kinase - dependent manner , is essential for hyperthermia - induced HSP72 expression in association with cardioprotection , suggesting impairment of this signaling pathway in the STZ - induced diabetic heart , probably due to insulin deficiency . - pubmed
Simultaneous quantitation of the nucleotide analog adefovir , its phosphorylated anabolites and 2 '- deoxyadenosine triphosphate by ion - pairing LC / MS / MS . - pubmed
The study of nucleoside / tide pharmacology has been hampered by difficulties encountered when trying to develop LC / MS / MS methods for these polar analytes . - pubmed
In an attempt to identify a more convenient , selective and sensitive alternative to the analysis of the metabolism of radiolabeled parent nucleotide traditionally used for in vitro cell culture studies , an LC / MS / MS method was developed for the quantitative detection of adefovir and its phosphorylated metabolites in cellular samples . - pubmed
Ion - pairing reversed phase LC using tetrabutylammonium ( TBA ) and ammonium phosphate had the best compromise between chromatographic separation and positive mode MS / MS detection . - pubmed
A cross - validation showed comparable levels of adefovir and its metabolites were determined using either LC / MS / MS or radioactivity detection . - pubmed
fulgidus FEN employing flap and double - flap substrates indicates that binding interactions with the 3'-extrahelical nucleotide stabilise the ground state FEN - DNA interaction , leading to stimulation of comparative reactions at DNA concentrations below saturation with the single flap substrate . - pubmed
PATIENTS AND METHODS : Tumors from the Cancer Research UK Taxotere as Adjuvant Chemotherapy phase III trial were tested for HER-2 using current diagnostic procedures . - pubmed
Three six - carbon and nine - carbon aldehydes , natural plant volatiles produced by the plant lipoxygenase pathway , were tested for their insecticidal activity against five species of stored - product beetles in feeding , fumigation and combined bioassays . - pubmed
DHCR7 primarily catalyzes the reduction of 7 - dehydrocholesterol ( 7DHC ) to cholesterol . - pubmed
Neither use of perianesthetic medications such as labetalol , glycopyrrolate , or remifentanil , or ECT technical variables such as seizure duration or electrode placement were associated with orthostatic blood pressure drop in the multivariate model . - pubmed
Circulating oxidized LDL ( oxLDL ) levels are strongly correlated to LDL - cholesterol ( LDL - c ) and apolipoprotein - B100 ( apoB100 ) , making it difficult to disentangle their independent contributions to cardiovascular risk . - pubmed
Other major independent determinants of oxLDL were sex , HDL - cholesterol , and LDL particle size . - pubmed
After adjustment for age , sex , glucose tolerance status , and Framingham risk score , the oxLDL / apoB100 ratio was negatively related to FMD ( P = 0.017 ) . - pubmed
Carboxypeptidase Z ( CPZ ) links thyroid hormone and Wnt signaling pathways in growth plate chondrocytes . - pubmed
Carboxypeptidase Z ( CPZ ) removes carboxyl - terminal basic amino acid residues , particularly arginine residues , from proteins . - pubmed
CPZ contains a cysteine - rich domain ( CRD ) similar to the CRD found in the frizzled family of Wnt receptors . - pubmed
We have previously shown that thyroid hormone regulates terminal differentiation of growth plate chondrocytes through activation of Wnt-4 expression and Wnt / beta - catenin signaling . - pubmed
The Wnt-4 protein contains a C - terminal arginine residue and binds to CPZ through the CRD . - pubmed
The objective of this study was to determine whether CPZ modulates Wnt / beta - catenin signaling and terminal differentiation of growth plate chondrocytes . - pubmed
Our results show that CPZ and Wnt-4 mRNA are co - expressed throughout growth plate cartilage . - pubmed
Knockdown of either Wnt-4 or CPZ mRNA levels using an RNA interference technique or blocking CPZ enzymatic activity with the carboxypeptidase inhibitor GEMSA reduces the thyroid hormone effect on both alkaline phosphatase activity and Col10a1 mRNA expression . - pubmed
Overexpression of CPZ in growth plate chondrocytes also removes the C - terminal arginine residue from a synthetic peptide consisting of the carboxyl - terminal 16 amino acids of the Wnt-4 protein . - pubmed
Removal of the C - terminal arginine residue of Wnt-4 by site - directed mutagenesis enhances the positive effect of Wnt-4 on terminal differentiation . - pubmed
These data indicate that thyroid hormone may regulate terminal differentiation of growth plate chondrocytes in part by modulating Wnt signaling pathways through the induction of CPZ and subsequent CPZ - enhanced activation of Wnt-4 . - pubmed
PURPOSE : To determine the feasibility of combined long - term luteinizing hormone - releasing hormone agonist - based androgen suppressive therapy ( AST ) and dose escalation with high - dose - rate ( HDR ) brachytherapy for high - risk ( HRPC ) or very - high - risk prostate cancer ( VHRPC ) . - pubmed
Biophysical characteristics of the factor - bound nucleosomal arrays were determined by analytical agarose gel electrophoresis ( AAGE ) and single molecules were visualized by atomic force microscopy ( AFM ) . - pubmed
Interestingly , the study of chemically modified Hb for use as oxygen therapeutics has allowed for some basic understanding of extracellular Hb toxicity , particularly in the absence of functional clearance mechanisms and in circulatory antioxidant depleted states . - pubmed
The induction , not the maintenance , of HFS - induced LTP is blocked by a N - methyl - D - aspartate ( NMDA ) receptor antagonist , D-2 - amino-5 - phosphonopentanoic acid ( D - AP5 ) . - pubmed
Both the induction and maintenance of LTP of Abeta - fiber - evoked fEPSPs are inhibited by a ROS scavenger , either N - tert - butyl - alpha - phenylnitrone or 4 - hydroxy-2,2,6,6 - tetramethylpiperidine - N - oxyl . - pubmed
Ca ( 2 +)-modulated membrane guanylate cyclase in the testes . - pubmed
To date , the calcium - regulated membrane guanylate cyclase Rod Outer Segment Guanylate Cyclase type 1 ( ROS - GC1 ) transduction system in addition to photoreceptors is known to be expressed in three other types of neuronal cells : in the pinealocytes , mitral cells of the olfactory bulb and the gustatory epithelium of tongue . - pubmed
In this presentation , the authors review the existing information on the localization and function of guanylate cyclase with special emphasis on Ca ( 2 +)-modulated membrane guanylate cyclase , ROS - GC1 , in the testes . - pubmed
The role of ROS - GC1 and its Ca ( 2 +)-sensing modulators in the processes of spermatogenesis and fertilization are discussed . - pubmed
The inhibition of the SHH signaling pathway by the specific inhibitor cyclopamine abolished CRCC cell growth as assessed by cell counting , BrdU incorporation studies , fluorescence - activated cell sorting and beta - galactosidase staining . - pubmed
[ Effects of adiponectin gene SNP45T / G on changes of serum lipid ratios induced by high - carbohydrate / low - fat diet in healthy Chinese youth ] . - pubmed
OBJECTIVE : To investigate the effects of adiponectin gene ( APM1 ) SNP45T / G on serum lipid ratios and their responses to high - carbohydrate / low - fat ( HC / LF ) diet in healthy young Chinese . - pubmed
The ras gene family provides a global effect on gene expression by encoding small GTP - binding proteins which act as molecular switches connecting extracellular signals with nuclear transcription factors . - pubmed
Late Onset Central Hypoventilation Syndrome due to a Heterozygous Polyalanine Repeat Expansion Mutation in the PHOX2B Gene . - pubmed
This report describes a 6 year old girl with late onset central hypoventilation syndrome due to a heterozygous polyalanine repeat expansion mutation in the PHOX2B gene . - pubmed
Mangiferin was not a substrate for the cytosolic sulphotransferases . - pubmed
The ferric iron reducing ability of the glucuronidated reaction mixture was reduced , while the free radical scavenging abilities of mangiferin , utilising on - line post - column HPLC - DAD - DPPH and HPLC - DAD - ABTS + assays , were eliminated , providing further evidence that the catechol arrangement at C-6 and C-7 was the preferred site of conjugation . - pubmed
In contrast , expression of the afs1 gene was strongly expressed by the above four carbohydrates and less strongly induced by galactan . - pubmed
( 1 ) H - NMR analysis revealed that rAFS1 degraded arabinofuranosyl side chains that were both singly and doubly linked to the backbones of arabinoxylan and L - arabinan . - pubmed
On the other hand , rAFQ1 preferentially released arabinose linked to C-3 of single - substituted xylose or arabinose residues in the two polysaccharides . - pubmed
Risk of tacrolimus toxicity in CYP3A5 nonexpressors treated with intravenous nicardipine after kidney transplantation . - pubmed
Compared with the other two groups combined , CYP3A5 * 3 /* 3 cases had higher median dose - adjusted MaxC0 ( 330 vs. 175 , P=0.012 ) , less time to MaxC0 ( 42 vs. 72 hr , P<0.001 ) , and more scheduled tacrolimus doses held per patient ( 1.75 vs. 0.4 , P=0.007 ) . - pubmed
CONCLUSION : CYP3A5 nonexpressors simultaneously treated with tacrolimus and CIVN may be at increased risk for tacrolimus toxicity . - pubmed
The present study examined the effects of 4 - phenylbutyric acid ( 4 - PBA ) , an ER chaperone , on acini and UPR components . - pubmed
Effects of 4 - PBA were measured on secretion , calcium , and trypsin activation . - pubmed
RESULTS : 4 - Phenylbutyric acid led to an increase of secretion , whereas trypsin activation with supraphysiological cholecystokinin was significantly reduced . - pubmed
4 - Phenylbutyric acid prevented chaperone - binding protein up - regulation , diminished protein kinaselike ER kinase , and c - Jun NH2 - terminal kinase phosphorylation , prohibited X - box - binding protein 1 splicing and CCAAT / enhancer - binding protein homologous protein expression , caspase 3 activation , and apoptosis caused by supraphysiological cholecystokinin . - pubmed
CONCLUSION : By incubation with 4 - PBA , beneficial in urea cycle deficiency , it was possible to enhance enzyme secretion to suppress trypsin activation , UPR activation , and proapoptotic pathways . - pubmed
A conserved motif in the ITK PH - domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions . - pubmed
Binding of the membrane phospholipid phosphatidylinositol 3,4,5 - trisphosphate ( PIP ( 3 ) ) to the Pleckstrin Homology ( PH ) domain of the Tec family protein tyrosine kinase , Inducible T cell Kinase ( ITK ) , is critical for the recruitment of the kinase to the plasma membrane and its co - localization with the TCR - CD3 molecular complex . - pubmed
However , the biological significance of the FYF amino acid motif in the ITK - PH domain is unknown . - pubmed
Here we identify the SWI / SNF ATP - dependent nucleosome - remodeling complex as a component of the RNA - mediated transcriptional silencing pathway . - pubmed
Induced pluripotent stem cells mediate the release of interferon gamma - induced protein 10 and alleviate bleomycin - induced lung inflammation and fibrosis . - pubmed
Administration of either 3 - gene iPSCs or iPSC - CM significantly attenuated collagen content and myeloperoxidase activity , diminished neutrophil accumulation , and rescued pulmonary function and recipient survival after bleomycin treatment . - pubmed
Notably , both treatments reduced the levels of inflammatory cytokines and chemokines , including interleukin 1 ( IL-1 ) , IL-2 , IL-10 , tumor necrosis factor - alpha , and monocyte chemotactic protein 1 yet increased the production of the antifibrotic chemokine interferon - gamma - induced protein 10 ( IP-10 ) in bleomycin - injured lungs . - pubmed
IR - induced overexpression of UCP2 was suppressed by ramiprilat and losartan . - pubmed
CONCLUSION : These findings suggest that losartan and ramiprilat can suppress UCP2 expression following myocardial IR , and by this mechanism may protect the myocardium against IR injury . - pubmed
Single - layer magnesium fluoride ( MgF ( 2 ) ) and antireflective ( AR ) coating at 193 nm were fabricated on a convex conical substrate holder ( with diameter 225 mm , apex angle 140 deg , and height 41 mm ) by thermal evaporation . - pubmed
From the reflectance spectra , uniform optical performance was also obtained for the 193 nm AR coating deposited on fused - silica substrates . - pubmed
From the beginning of treatment to the CT and at d8 , d15 and d30 , oral morphine equivalent dose ( OMED ) did not significantly differ . - pubmed
BACKGROUND : Developing a round - the - clock artificial pancreas requires accurate and stable continuous glucose monitoring . - pubmed
The radiochemical yield of the [ 1 -( 11 ) C ] Tpi - containing RGD at the end of synthesis ( EOS ) was 5.9 + - 1.9 % ( n = 4 ) , for a total synthesis time of about 35 min . - pubmed
While the PAPS - bound enzyme spends the majority ( 95 % ) of its time in the constricted state , the pore isomerizes between the open and closed states when the nucleotide ( PAPS ) is bound . - pubmed
Creatine kinase ( CK ) , aspartate aminotransferase ( AST ) , alanine aminotransferase ( ALT ) , gamma - glutamyl transpeptidase ( GGT ) , lactate dehydrogenase ( LDH ) , alkaline phosphatase ( AP ) , cortisol and C - reactive protein ( CRP ) , glutathione peroxidase ( GPx ) and reductase activities ( GR ) and C - reactive protein ( CRP ) levels , total antioxidant status , and cortisol levels were measured in serum the day before and immediately after the race . - pubmed
CK and LDH and cortisol levels were negatively correlated to total energy , protein , and fat intake . - pubmed
Intake of vitamin A , B1 , B2 , B6 and niacin was negatively correlated to LDH and AP . - pubmed
A negative correlation was also found between CK activity and Na , Fe , and Zn intake . - pubmed
A positive correlation was found between serum GPx and intake of energy , carbohydrates , proteins , A and B vitamins , and folic acid . - pubmed
Plasma proinflammatory cytokines and urinary F2 - isoprostanes were measured . - pubmed
Finally , suppression of PTPalpha resulted in increased phosphorylation of focal adhesion kinase on Tyr-407 , which induced the recruitment of vinculin and the formation of a novel focal adhesion kinase complex in response to ErbB2 activation in mammary epithelial cells . - pubmed
The alpha - fetoprotein fraction L3 ( AFP - L3 ) , which is synthesized by malignant cells and incorporates a fucosylated oligosaccharide , has been investigated as a diagnostic and prognostic marker for hepatocellular carcinoma ( HCC ) . - pubmed
ATG4 processes ATG8 precursor to expose its C - terminal glycine for phosphatidyl ethanolamine ( PE ) lipidation . - pubmed
GFP fusion proteins of ATG8s , especially of ATG8s with innate C - terminal - exposed glycines , localized to punctate autophagic membranes . - pubmed
Among numerous fructofuranose conformers discovered ( about 250 ) , a pair of the ( ( 3 ) T2 ) alpha - and ( E3 ) beta - anomers are energetically most preferred and lie at least 12 kJ / mol above the global minimum . - pubmed
The commonly used M06 - 2X density functional performs well compared to MP2 and G4 theory at identifying the low - energy fructose minima , including the global one , and at reproducing their intramolecular H - bond geometric parameters . - pubmed
The purified IgG , unlike native sera , mirrored IVIg reactivity to a wide array of HLA - I /- II alleles , indicating that anti - HLA IgG may be masked in normal sera - either by peptides derived from soluble HLA or by those from antibodies . - pubmed
Comparison of HLA types with the profile of HLA antibodies showed auto - HLA IgG to the donors ' HLA antigens in this order of frequency : DPA ( 80 % ) , DQA ( 71 % ) , DRB345 ( 67 % ) , DQB ( 57 % ) , Cw ( 50 % ) , DBP ( 43 % ) , DRB1 ( 21 % ) , A ( 14 % ) and B ( 7 % ) . - pubmed
At 1 h post feeding , L - Arg mares had lower ( P<0.05 ) plasma concentrations of histidine , glutamic acid , proline , isoleucine , threonine , phenylalanine , leucine , valine , alanine , and taurine . - pubmed
The mutant Rht - B1c displayed lower and slower electrolyte leakage in comparison with the wild - type Rht - B1a . - pubmed
It is shown that when the growth substrate temperature is sufficiently high , a portion of a Ag NP can be melted for serving as the catalyst to precipitate GaZnO on the residual Ag NP and form a GaZnO NN . - pubmed
Such superior field emission performances are attributed to a few factors , including ( 1 ) the low work function and high conductivity of the grown GaZnO NNs due to highly degenerate Ga doping , ( 2 ) the sharp - pointed geometry of the vertically aligned GaZnO NNs , ( 3 ) the Ag doping in VLS precipitation of GaZnO for further reducing NN resistivity , and ( 4 ) the residual small Ag NP at the NN tip for making the tip even sharper and tip conductivity even higher . - pubmed
Design , synthesis , in - vitro antiproliferative activity and kinase profile of new picolinamide based 2 - amido and ureido quinoline derivatives . - pubmed
Isotretinoin Laboratory Test Monitoring -- A Call to Decrease Testing in an Era of High - Value , Cost - Conscious Care . - pubmed
Furthermore , we uncovered Dfd as a potential regulator of synaptic specificity , as it represses expression of the synaptic cell adhesion molecule Connectin ( Con ) . - pubmed
Two died while receiving high - dose systemic corticosteroids , methotrexate , and after recent exposure to anti - tumor necrosis factor - alpha biological DMARDs , and 2 during hematopoietic stem cell transplantation procedure . - pubmed
Infliximab and adalimumab are anti - TNF - alpha antibodies that specifically block the interaction of TNF - alpha with its receptors . - pubmed
Vitamin A Transport and Cell Signaling by the Retinol - Binding Protein Receptor STRA6 . - pubmed
Vitamin A , retinol , circulates in blood bound to retinol binding protein ( RBP ) . - pubmed
In some tissues , the retinol - RBP complex ( holo - RBP ) is recognized by a membrane receptor , termed STRA6 , which mediates uptake of retinol into cells . - pubmed
Recent studies have revealed that , in addition to serving as a retinol transporter , STRA6 is a ligand - activated cell surface signaling receptor that , upon binding of holo - RBP activates JAK / STAT signaling , culminating in the induction of STAT target genes . - pubmed
It has further been shown that retinol transport and cell signaling by STRA6 are critically interdependent and that both are coupled to intracellular vitamin A metabolism . - pubmed
The formation of BPA intermediates was investigated in detail by using high - performance liquid chromatography - mass spectrometry ( HPLC / MS ) . - pubmed
A covariance analysis was conducted to analyze differences of oxygenated hemoglobin ( oxy - Hb ) changes in prefrontal cortex ( PFC ) between the two groups and reduce the influence of the severity of depression . - pubmed
As a result of this study , we have identified ProxyPhos library members that are most suitable for the detection of proximally phosphorylated peptides , PPi , UTP , and a DpYD peptide motif , and have generally provided a foundation for the selection of ProxyPhos chemosensors for further development of specific biologically relevant assays . - pubmed
Expression of PLA2 was significantly reduced following treatment with 28 - homobrassinolide . - pubmed
In general , flavanols and phenolic acids exhibited their maximum concentration at week 5 and then decreasing significantly during the rest of maturation . - pubmed
Focal dose escalation for prostate cancer using 68Ga - HBED - CC PSMA PET / CT and MRI : a planning study based on histology reference . - pubmed
The aim of this study was to demonstrate the feasibility and to evaluate the tumour control probability ( TCP ) and normal tissue complication probability ( NTCP ) of radiation therapy dose painting using 68Ga - HBED - CC PSMA PET / CT , mpMRI or the combination of both in primary PCa . - pubmed
Typical management of VWD patients is also discussed , including the use of desmopressin and VWF concentrates . - pubmed
Src is a tyrosine kinase known to both phosphorylate Cx43 ( residues Y247 and Y265 ) and affect gap junction intercellular communication . - pubmed
However , the Cx43 carboxyl - terminal ( CT ) domain contains additional tyrosine residues and proteomic discovery mass spectrometry data identified Y313 as a potential phosphorylation target . - pubmed
, which still observed tyrosine phosphorylation by Src when using a Cx43 Y247 / Y265F mutant , we addressed the possibility of Y313 phosphorylation ( pY313 ) by Src . - pubmed
When v - Src was over - expressed , Cx43 WT localized intracellularly , while all of the single and double mutants remained able to form plaques and transfer dye , albeit variable in number and amount , respectively . - pubmed
In addition to conventional diagnostics , the measurement of enhanced tumor cell metabolism using F-18 fluorodeoxyglucose ( 18F - FDG ) for increased glycolysis or C-11 acetate for fatty acid synthesis by positron emission tomography / computed tomography ( PET / CT ) is well established for clinical management of HCC . - pubmed
Objective : A putative relationship between markers for the serotonin system and the personality scale self - transcendence ( ST ) and its subscale spiritual acceptance ( SA ) has been demonstrated in a previous PET study of 5 - HT1A receptor binding in healthy control subjects . - pubmed
NBT treatment decreased the expression of a neuroblastoma - derived MYC ( MYCN ) and multidrug resistance - associated protein 1 ( MRP1 ) as well as downregulating Akt , GSK3beta , and beta - catenin . - pubmed
Through subsequent sequence analysis and thermal shift assays , it was found that the purine / pyrimidine content modulates the overall stability of the polymerase complex , resulting in alteration of the activity of viral RNA synthesis . - pubmed
Appearances of pigment changes and hemorrhages were some of the differences found when compared to the conventional CFP . - pubmed
Consistent with this , stimulation of endothelial cells ( EC ) with thrombin caused an increase in BiP / GRP78 levels and inhibition of ER stress with 4 - phenylbutyric acid ( 4 - PBA ) prevented this response as well as increase in VCAM-1 , ICAM-1 , IL-6 , and IL-8 levels . - pubmed
Importantly , thrombin - induced Ca2 + signaling and EC permeability were also prevented upon BiP / GRP78 inactivation . - pubmed
The aim of this study was to optimize a multistep Triton X-100 based protocol ( either using Triton X-100 / ammonium hydroxide mixture alone or after its modification with DNase , sodium dodecyl sulfate or trypsin ) that could achieve maximum decellularization with minimal liver ECM destruction suitable for subsequent organ implantation without immune rejection . - pubmed
Among all Triton X-100 modified protocols , a Triton X-100 / DNase - based one was considered the most suitable . - pubmed
The twin - arginine translocation ( Tat ) system transports folded proteins across membranes of prokaryotes , plant plastids , and some mitochondria . - pubmed
This complex was initially found when the photo cross - linking amino acid p - benzoyl - l - phenylalanine ( Bpa ) was introduced at position I50 on the periplasmic side of the first trans - membrane domain of TatC. - pubmed
Lactate dosage was performed at ED - HDU admission ( T0 ) , after 2 h ( T2 ) , 6 h ( T6 ) and 24 h ( T24 ) ; lactate clearance was calculated at T2 and T6 [ T2 : ( ( LAC T0 - LAC T2 / LAC T0)*100 ) ] ; T6 : [ ( LAC T0 - LAC T6 / LAC T0)*100 ] . - pubmed
The G protein - coupled estrogen receptor ( GPER ) is a seven - transmembrane - domain receptor that mediates non - genomic estrogen related signaling . - pubmed
Therefore , a better understanding of the role GPER plays in cancer biology may lead to the identification of novel therapeutic targets , especially among estrogen - related cancers . - pubmed
Median progression - free survival ( PFS ) for the first remission was 105 days ; lack of expression of CD3 on flow cytometry ( P < .0001 ) and pretreatment with steroid ( P = .012 ) were significantly associated with shorter PFS . - pubmed
An experimental design with four factors : enzyme / substrate ratio , quantity of xylose , hydrolysis and cooking times was used to create a range of enzymatic hydrolysates . - pubmed
DNA sequencing for the beta - globin gene revealed rare a -90 ( C > T ) ( HBB : c.-140 C > T ) mutation for the woman . - pubmed
We also developed an immunohistochemical stain for EFEMP1 applicable to formalin - fixed tissue sections and performed electron microscopy in one case . - pubmed
Although the EFEMP1 deposits were weakly to moderately Congo red - positive with absent to weak birefringence , they were strongly positive for EFEMP1 by immunohistochemistry , had the characteristic fibrillar ultrastructure of amyloid , and were readily identified by mass spectrometry . - pubmed
Because EFEMP1 amyloid is only weakly Congo red positive it may be overlooked without a high index of suspicion . - pubmed
Based on its distinctive features , we propose that EFEMP1 amyloidosis be considered a new amyloid type . - pubmed
AIM : Treatment with obeticholic acid ( OCA ) affects the blood lipid profile . - pubmed
Six ( 6 ) articles with 10 trials for low - density lipoprotein cholesterol ( LDL - C ) , and 8 trials for high - density lipoprotein cholesterol ( HDL - C ) , total cholesterol ( TC ) , and triglycerides ( TG ) levels were included in the meta - analysis . - pubmed
The pooled results showed that the levels of TC ( WMD : 6.357 mg / dl ) and LDL - C ( WMD : 6.067 mg / dl ) increased while TG ( WMD : -22.417 mg / dl ) decreased after treatment with OCA . - pubmed
METHODS : We use patch clamp electrophysiology in acute cerebellar slices and immunostaining to identify the specific ion channels targeted by chlorzoxazone - baclofen . - pubmed
ILA ( 1 - 10 mM ) significantly attenuated lipopolysaccharide ( LPS)-induced activation of NF - kB in macrophages . - pubmed
ILA significantly attenuated TNF - alpha- and LPS - induced increase in the pro - inflammatory cytokine IL-8 in intestinal epithelial cells . - pubmed
ILA increased mRNA expression of the aryl hydrogen receptor ( AhR)-target gene CYP1A1 and nuclear factor erythroid 2 - related factor 2 ( Nrf2)-targeted genes glutathione reductase 2 ( GPX2 ) , superoxide dismutase 2 ( SOD2 ) , and NAD ( P ) H dehydrogenase ( NQO1 ) . - pubmed
In addition , we assessed whether there were any associations between an increase in oxygen requirement and known risk factors for critical illness in COVID-19 , including elevation of liver enzymes and C - reactive protein ( CRP ) , lymphocytopenia , high D - dimer levels and the chest computed tomography ( CT ) score . - pubmed
Comorbid cardiovascular disease , lymphocytopenia , elevated CRP , liver enzyme and D - dimer levels , and higher chest CT score were significantly associated with an increase in oxygen requirement CONCLUSIONS : The expanded CURB-65 score can be a better predictor of an increase in oxygen requirement in patients with SARS - CoV-2 pneumonia . - pubmed
Synthetic thick filaments : A new avenue for better understanding the myosin super - relaxed state in healthy , disease , and mavacamten - treated cardiac systems . - pubmed
Using these STFs , we show several pathophysiological insults in hypertrophic cardiomyopathy , including the R403Q myosin mutation , phosphorylation of myosin light chains , and increased ADP : ATP ratio destabilize the SRX population . - pubmed
Furthermore , wild - type myosin containing STFs , but not S1 , HMM , or STFs - containing R403Q myosin , recapitulated the ADP - induced destabilization of the SRX state . - pubmed
Studies involving a clinical - stage small molecule inhibitor , mavacamten , showed that it is not only more effective in increasing myosin SRX population in STFs than in S1 or HMM , but it also increases myosin SRX population equally well in STFs made of healthy and disease - causing R403Q myosin . - pubmed
Importantly , we also found that pathophysiological perturbations such as elevated ADP concentration weakens the mavacamten ' s ability to increase the myosin SRX population , suggesting that mavacamten - bound myosin heads are not permanently protected in the SRX state but can be recruited into action . - pubmed
Antipsychotic potential of the type 1 cannabinoid receptor positive allosteric modulator GAT211 : preclinical in vitro and in vivo studies . - pubmed
In this study , we compared the activity and stability of in situ or crosslink - immobilized enzymes in a CaCO3 biomineral carrier . - pubmed
More than 30 % of the initial enzyme activity was preserved for both the systems after 180 days upon 15 activity measurements at room temperature , confirming the improved stability of these enzyme systems ( 100 mM phosphate buffer , pH 8.0 ) ; however , differences in enzyme loading , activity , and characteristics were observed for each of these methods . - pubmed
Based on the same amount of immobilized enzyme ( 0.2 mg ) , the specific activities of hydrolysis of p - nitrophenyl butyrate substrate at room temperature of in situ immobilized carboxyl esterase ( CE ) and crosslinked CE were 11.37 and 7.63 mM min-1 mg-1 , respectively ( 100 mM phosphate buffer , pH 8.0 ) . - pubmed
Moreover , based on the kinetic behavior , in situ immobilized CE exhibited improved catalytic efficiency ( Vmax Km-1 ) of the enzyme , exhibiting 4 - fold higher activity and efficiency values than those of the CE immobilized in CaCO3 . - pubmed
This is the first study to describe the stabilization of enzymes in CaCO3 and compare the enzyme kinetics and efficiencies between in situ immobilization and crosslinking in CaCO3 carriers . - pubmed
In the case of PHA , increased neoAg expression was correlated with an increased incorporation of 14C - leucine into C5 , C6 , C7 , and C8 antigens , which was detected by immunodiffusion and autoradiography . - pubmed
That naloxone alters the depth of inhalational anesthesia suggests that anesthetics may release an endogenous morphine - like factor ( MLF ) in the central nervous system . - pubmed
Intracellular Ca2 + concentration ( [ Ca2+]i ) was raised by photolysis of a caged calcium compound , nitr-5 , and its effects on the mechano - electrical transduction ( MET ) current were studied by a whole - cell patch electrode voltage clamp technique in dissociated hair cells of a chick . - pubmed
Nitr-5 was loaded into the hair cell by incubation with the membrane - permeable form of the compound ( nitr-5 AM ) . - pubmed
The extent of MET current decay was reduced or disappeared in 20 - 100 microM Ca2 + extracellular media and the offset time course was prolonged at the membrane potential of -50 mV. - pubmed
These results ( paragraphs 3 - 6 ) suggest that the MET current adaptation is accelerated by the increase of [ Ca2+]i , and that Ca2 + ions entering through MET channels are essential in the development of adaptation . - pubmed
The adaptation of the MET current was reversibly reduced in a dihydrostreptomycin ( DHSM , 20 - 50 microM ) medium . - pubmed
These delays in the development and the subsidence of adaptation suggest a presence of a Ca2 + buffer site intracellularly between the adaptative site and the MET channel . - pubmed
Cyclophosphamide reduced the arthritis , together with decreases in the lymphocyte count and the serum IgG level . - pubmed
Octreotide given before the induction of pancreatitis significantly reduced the levels of serum amylase and lipase , ascites amylase concentration , degree of leukocyte infiltration , and focal areas of pancreatic tissue necrosis . - pubmed
Using ciprofloxacin concentrations adjusted for a higher MIC obtained in pH - adjusted urine and MHB , PAE values were similar for urine and MHB ( approximately 280 min at 40 x MIC ) . - pubmed
Selenium had no effect on 5 - aminolevulinic acid synthetic ability but inhibited 5 - aminolevulinic acid dehydratase and PBG deaminase activities . - pubmed
The results suggest the possible regulatory role of selenium on chlorophyll synthesis by interacting with sulfhydryl containing enzymes 5 - aminolevulinic acid dehydratase and porphobilinogen deaminase . - pubmed
The effects of cisplatin on electrical and mechanotransducer properties of the crayfish stretch receptor neuron was studied using a microelectrode voltage clamp technique . - pubmed
The need for extracellular calcium , calcium influx , and specifically for calcium influx through the calcium channel was examined . - pubmed
Nifedipine , a dihydropyridine calcium channel antagonist , significantly ( P less than 0.01 ) reduced angiotensin II- and potassium - stimulated aldosterone secretion , but had no effect on dbcAMP - stimulated aldosterone secretion ( 100 + /- 14 vs 105 + /- 19 pmol/10(6 ) cells ) . - pubmed
Angiotension II and potassium activation of both the early aldosterone biosynthetic pathway ( as reflected by pregnenolone production in the presence of cyanoketone ) and also its late pathway ( as reflected by the conversion of exogenous corticosterone to aldosterone in the presence of cyanoketone ) were significantly ( P less than 0.01 ) inhibited by lanthanum , nifedipine and by reducing the extracellular calcium concentration . - pubmed
These observations suggest that : the extracellular calcium dependency of dbcAMP- , angiotensin II- and potassium - stimulated aldosterone secretion reflects a need for calcium influx ; with dbcAMP stimulation , activation of the late pathway is dependent on calcium influx by a calcium channel - independent mechanism , whereas activation of the early pathway is not dependent on extracellular calcium or calcium influx and activation of both the early and late pathway by angiotensin II and potassium is dependent on calcium influx by a calcium channel - dependent mechanism . - pubmed
The specimens were fixed in glyceraldehyde , washed in a cacodylate buffer , post - fixed in potassium ferrocyanide reduced - osmium tetroxide , stained with uranyl acetate and included in EPON 812 . - pubmed
In frontal cortex , the affinity of opiate receptors for dihydromorphine was significantly reduced in brains of alcoholics . - pubmed
[ Experiences with L - carnitine in the post - stress phase ] . - pubmed
Beside carnitine and acetylcarnitine levels in plasma and urine the following parameters were determinated to evaluate the effect of carnitine : for the metabolism of fatty acids : triglycerides , free fatty acids ( FFA ) , alpha - hydroxy - butyrate for the metabolism of carbohydrates : glucose , insulin and lactate in plasma . - pubmed
Finally for amino acid metabolism : urea , creatinine , cholinesterase and kolloid osmotic pressure in plasma as well as ureanitrogen and alpha - aminonitrogen excretion in urine . - pubmed
Thus , while P-450PCN - E may be oligomeric in microsomal membranes , target inactivation analysis does not appear to give conclusive results regarding the states of aggregation of these microsomal proteins . - pubmed
Analysis of the structure - activity relationships indicated that , for maximum biological activity , steroids must have a planar nucleus ( as in delta 4 and 5 alpha - reduced steroids ) , a hydroxyl or keto group at position 3 , and hydroxyl groups at positions 17 and 20 beta . - pubmed
Previous studies have shown that the central nervous system is able to convert testosterone into 17 - beta - hydroxy-5 - alpha - androstan-3 - one ( DHT ) , by the action of the enzyme 5 - alpha - reductase . - pubmed
Ki values calculated for taurocholate ( 43 microM ) and testosterone glucuronide ( 28 microM ) indicated that these two inhibitors were relatively weak competitors for this E2 17 G transport site . - pubmed
A dose - related decrease in ciliary beating frequency ( CBF ) was seen after perfusion with both BDP and flunisolide as well as propylene glycol . - pubmed
The decrease in CBF following perfusion with propylene glycol was partially reversible upon re - perfusion with medium alone , whereas the decrease seen after BDP and flunisolide was irreversible . - pubmed
Amiodarone has been found to decrease serum T3 by blocking peripheral T4 5 '- deiodinase . - pubmed
The value for MPF was determined by the radioactive microsphere method and PS was assessed by an integral tissue - uptake method using [ 51Cr ]- EDTA as test molecule . - pubmed
During coronary vasodilation achieved by intra - coronary infusion of adenosine , MPF increased markedly from 101.5 + /- 14.2 to 778.1 + /- 135.2 ml X min-1 X 100 g-1 in subendocardial layers and from 77.7 + /- 10.3 to 635.3 + /- 85.9 ml X min-1 X 100 g-1 in subepicardial layers of the left ventricular wall . - pubmed
Raising MPF by 800 % was accompanied by a modest but statistically significant increase in PS [ 51Cr ]- EDTA . - pubmed
The effects of chlorpromazine , haloperidol , and thioridazine on brain noradrenaline content elevated by previous administration of MAO inhibitors . - pubmed
They are prostaglandin synthetase inhibitors , three to ten times less active as indomethacin . - pubmed
Within 15 min of nicorandil administration , heart rate increased and peripheral resistance decreased ( dose - dependent ) ; the preejection period ( corrected ; PEPc ) and the ratio of PEP to left ventricular ejection time ( LVET ) - PEP / LVET - were shortened by the 60 mg dose ; the heart rate corrected electromechanical systole ( QS2c ) was almost unchanged ; left ventricular end diastolic and systolic diameters ( EDD and ESD ) were diminished . - pubmed
The effects on certain parameters had not completely disappeared after 6 h. ISDN produced a similar pattern with somewhat less intense effects ; although PEPc and PEP / LVET were prolonged . - pubmed
The predominant action of ISDN was to reduce preload and thereby to lengthen PEPc and PEP / LVET ; in addition , it lessened afterload . - pubmed
Although nicorandil caused a distinct reduction in preload , as shown by the smaller EDD , the effects of afterload reduction were predominant at the high dose induced shortening of PEPc and PEP / LVET . - pubmed
Dense deposits revealed by electron microscopy were found in the regions where IF of IgG and C3 was observed . - pubmed
Acid buffer eluates from liver and kidney contained IgG reactive with nuclear antigens . - pubmed
The positive relationship between Cl1MU and Cl3MX in both smokers and nonsmokers ( r = 0.98 , P less than 0.001 ) supports the concept that two N - demethylation pathways for theophylline metabolism are under common regulatory control and involve a form of cytochrome P450 distinct from that mediating 8 - hydroxylation of theophylline to DMU . - pubmed
These results suggest that cigarette smoking induces both of the cytochrome P450 - mediated pathways of theophylline metabolism but that N - demethylation may be increased to a greater extent than 8 - hydroxylation . - pubmed
All the neutral glycosphingolipids were isolated and their structure proved by the quantitative determination of their components , degradation by acid and specific glycohydrolases and permethylation and gas - liquid chromatographic - mass spectrometric assay of the methylated sugars . - pubmed
In fibroblasts , growth factor stimulation results in the recruitment of Grb2 - mSos1 into complexes with activated receptors and cytoplasmic phosphoproteins such as Shc , which are apparently involved in Ras activation , and subsequently to an increase in mSos1 phosphorylation on serine and threonine . - pubmed
These common phosphorylation sites have been mapped to a region encompassing the first three proline ( pro)-rich motifs in the tail of mSos1 . - pubmed
These findings suggest a potential inhibitory role for MAP - kinase in attenuating nucleotide exchange on Ras , by uncoupling mSos1 from membrane - bound receptor complexes that lead to Ras activation . - pubmed
The mechanism of action of aspirin -- is there anything beyond cyclo - oxygenase ? - pubmed
Most workers agree that aspirin inhibits the cyclo - oxygenase enzyme in the platelets leading to reduced formation of prostaglandin G2 , the precursor of thromboxanes . - pubmed
Competition radioimmunoassay ( CRIA ) revealed Ab2 specificities towards Ab1 antigen binding sites by inhibition of HBsAg / Ab1 binding . - pubmed
The androgen receptor belongs to the family of nuclear receptors and contains three functional domains : a carboxy - terminal hormone binding region , a central cystein rich DNA binding region and an amino - terminal region involved in the expression of androgen regulated genes . - pubmed
Cloning of the complementary DNA encoding the androgen receptor enabled the characterization of the molecular defects associated with androgen insensitivity syndromes , X - linked disorders resulting from androgen action defects in target cells . - pubmed
The cis - effect of a nascent peptide on its translating ribosome : influence of the cat-86 leader pentapeptide on translation termination at leader codon 6 . - pubmed
The inducer chloramphenicol stalls a ribosome at a specific site in the leader of cat transcripts ; this destabilizes a downstream stem - loop structure that normally sequesters the ribosome - binding site for the cat structural gene . - pubmed
Our evidence indicates that the cat-86 leader pentapeptide can alter the function of its translating ribosome . - pubmed
An absent or negative inotropic response to isoproterenol is observed in SHR - F and SHR - NF papillary muscles and may result in part from age - related alterations in beta - adrenergic receptor dynamics and a shift from alpha- to beta - myosin heavy chain ( MHC ) protein . - pubmed
The renin - angiotensin - aldosterone system is also implicated in the transition to failure : SHR treated with the angiotensin converting enzyme inhibitor captopril starting at 12 months of age did not develop heart failure during the 18 - 24 month observation period . - pubmed
Research opportunities include investigation of apoptosis as a mechanism of cell loss , delineation of the regulatory roles of TGF - beta 1 and the renin - angiotensin - aldosterone system in matrix accumulation , and studies of proteinase cascades that regulate matrix remodelling . - pubmed
Such a distribution seems to be responsible for the transcellular transport of Pgp substrates , including cyclosporin A ( CsA ) , from the basal to the apical side . - pubmed
Nevertheless , the sensitivity of their mitogen - induced proliferation to cytostatics , including doxorubicin and CsA , could be increased by the Pgp blockers . - pubmed
The Pgp blockers can restore the CsA retention in the MDR cells to its level in the Par cells . - pubmed
For instance , activating mutations of the Gs protein , leading to constitutive stimulation of the cAMP pathway , have been found in a subset of GH - secreting adenomas . - pubmed
But GHRH ( growth hormone - releasing hormone ) tumoral hypersecretion also stimulates the cAMP pathway and causes acromegaly . - pubmed
On acute stage , 99mTc - ECD brain SPECT disclosed diffuse high uptake in the cerebral cortex in spite of normal findings on brain X - ray CT . - pubmed
The total cellular p185 ( HER-2 / neu ) protein ( p185 ) content was measured by ELISA in 346 invasive primary breast cancers , and the results were compared with those of estrogen ( ER ) and progesterone ( PR ) receptors , pS2 and Cathepsin D ( Cat D ) content . - pubmed
Our results demonstrate for the first time that all SP - immunoreactive boutons in all cortical layers contain GABA . - pubmed
Determination of the amino - terminal amino acid sequence showed it to be identical to that predicted from the derived sequence for the argR gene . - pubmed
Quantitative S1 nuclease experiments showed that expression of the first gene in this operon , aruC , is initiated from an arginine - inducible promoter . - pubmed
Our preliminary findings showed that geiparvarin and some derivatives obtained from its conjugation with diethylstilboestrol and meso - hexestrol significantly inhibit taxol - induced in vitro polymerization of both tubulin and microtubular protein . - pubmed
Matrix metalloproteinase-3 ( MP-3 or stromelysin-1 ) specifically hydrolyzes the Glu59 - Asn60 , Pro447 - Val448 , and Pro544 - Ser545 peptide bonds in plasminogen , yielding a 55 kDa NH2 - terminal angiostatin - like domain ( comprising kringles 1 - 4 ) , a 14 kDa domain comprising kringle 5 , and a 30 kDa domain comprising the serine proteinases domain . - pubmed
The conversion is completely abolished in the presence of the MMP inhibitors EDTA or 1,10 - phenanthroline . - pubmed
By contrast , TNF was a very poor activator of p42 / 44 MAP kinase relative to the known activator of p42 / 44 MAP kinase in endothelial cells , adenosine triphosphate ( ATP ) . - pubmed
TNF - stimulated activation of p38 MAP kinase , and MAPKAP kinase-2 , a known downstream target of p38 MAP kinase , was strongly inhibited by pre - incubation with the p38 MAP kinase inhibitor SB203580 , whereas the minor activation of p42 / 44 MAP kinase was abolished by pre - incubation of the cell with the novel MAP kinase kinase 1 inhibitor PD098059 . - pubmed
Pre - incubation with PD098059 or co - incubation with ATP failed to modify the inhibitory effect of TNF upon DNA synthesis . - pubmed
SB203580 reduced basal DNA synthesis by approximately 50 % ; however , if failed to modify the inhibition mediated by TNF . - pubmed
Binding of CD4 ligands induces tyrosine phosphorylation of phosphatidylinositol-3 kinase p110 subunit . - pubmed
Since these results suggest a relationship between p56lck and PI3 - kinase , we investigated whether PI3 - kinase was tyrosine phosphorylated after CD4 binding and whether this phosphorylation was also dependent on the CD4 - p56lck association . - pubmed
We show herein that CD4 binding increased tyrosine phosphorylation of the catalytic subunit p110 of PI3 - kinase but not of the p85 subunit . - pubmed
In contrast , p110 tyrosine phosphorylation was inducible , transient and dependent on the CD4 - p56lck association . - pubmed
The role of the tyrosine phosphorylation of p110 - PI3 - kinase following ligand binding to CD4 is unknown . - pubmed
In muscles with myofiber atrophy and target fibers , we found an increased expression of the genes encoding for the ubiquitin - ATP - dependent proteolytic system . - pubmed
Comparisons of nucleotide and deduced amino acid sequences of NSP4 genes of virulent and attenuated pairs of group A and C rotaviruses . - pubmed
It was recently reported that NSP4 may also function as a viral enterotoxin in the induction of rotavirus diarrhea by causing Ca ++ influx in the cytoplasm of the infected cells . - pubmed
Analysis of the NSP4 deduced amino acid sequences showed that only 3 ( Wa ) and 2 ( M and Cowden ) amino acids differed between the virulent and attenuated strains . - pubmed
After considering the ratio between the number of T cells expressing Th- ( IFN - gamma)- and Th-2 ( IL-4)-associated cytokines in each individual , we found that there was a significant imbalance in this ratio , with a predominance of IFN - gamma - producing T cells over IL-4 + T lymphocytes during EtOH intake . - pubmed
Liver mitochondrial low - Km aldehyde dehydrogenase ( ALDH2 , EC 1.2.1.3 ) , the isoform responsible for the conversion of acetaldehyde to acetate , is inhibited by the sulfoxide bioactivation products of Et2NC ( O ) SMe ( from the alcohol aversion drug disulfiram ) , Pr2NC ( O ) SEt ( the herbicide S - ethyl N , N - dipropylthiocarbamate ) , and BuNHC ( O ) SMe ( from the fungicide benomyl ) . - pubmed
The labeled protein was isolated by isoelectric focusing ( pI 6.1 ) and SDS - PAGE ( 54 kDa ) and identified as ALDH2 by sequencing of peptides from a tryptic digest . - pubmed
These findings are of interest relative to selective inhibitors and carbamoylating agents for ALDH2 and to alcohol aversion upon exposure to herbicides and fungicides . - pubmed
Ly-49G2 blocks activation mediated by Ly-49D in an immunoreceptor tyrosine - based inhibitory motif ( ITIM)-dependent manner . - pubmed
This general amino acid control response is based on de - repressed translation of GCN4 mRNA , which is induced by the activation of the eIF2alpha kinase , GCN2 . - pubmed
Thus , GI domain - mediated association of GCN2 to GCN1 is required for general amino acid control . - pubmed
Histological sections and scanning electron microscopy of GP / Bc eyes during the normal period of eyelid growth and fusion indicate that gp / gp mutant fetuses have deficient upper and lower eyelids ; surface periderm cells that appear to have some role in eyelid growth and fusion are present , but lack a normal " streaming pattern toward the fusion zone . - pubmed
Both the catechol - O - methyltransferase ( COMT ) inhibitor entacapone and the monoamine oxidase B ( MAO - B ) inhibitor selegiline are L - dopa extenders . - pubmed
or both entacapone and selegiline with the L - dopa / DDC inhibitor medication . - pubmed
Oral administration of TN ( 50 - 100 mg / kg ) dose - dependently prevented ulcer formation by HCl / ethanol ( 36 - 75 % ) , absolute ethanol ( 43 - 75 % ) , HCl - ethanol / indomethacin ( 58 - 84 % ) , Pylorus ligation ( 31 - 100 % ) , cold restraint stress ( 68 - 100 % ) and histamine ( 49 - 100 % ) . - pubmed
Augmented histamine - induced gastric acid secretion was reduced from 84 mEq / l in the controls to 45 and 21 mEq / l for the two doses of TN , with total inhibition of gastric and duodenal ulcers by the 50 mg / kg dose . - pubmed
Healing rate of chronic acetic acid - induced ulcers was 62 and 83 % , respectively , for the dose of 50 and 100 mg / kg of TN compared with the controls . - pubmed
[ The experimental study of Sr - HAP on reconstructing mandibular bone defect ] . - pubmed
RESULTS : Sr - HAP had hardly been rejected by hosts , and in early period after operation the new bone in the Sr - HAP was found with great quantities as the degradation of Sr - HAP much more than that in the pure HA , and the difference of bone quantity can be shown in statistics . - pubmed
Sr - HAP has a better biocompatibility , biodegradation than pure HA and a excellent osteoinductivity . - pubmed
The existence of Sr - HAP improves the total new bone quantity and the interface of bone , also prolongs the period of new bone increasing . - pubmed
Sr - HAP has a good biodegradation and suitable speed of biodegradating , so the reconstruction effect with Sr - HAP is fairly satisfactory . - pubmed
This study was undertaken to determine whether casein , compared with its constituent amino acids , given at the onset of a meal , would influence intake due to cholecystokinin ( CCK ) or opioid activity . - pubmed
We investigated the hypothesis that inhaled nitric oxide ( iNO ) and hyperoxia each decreases lung vascular endothelial growth factor ( VEGF ) expression but increases endostatin and that concurrent administration of both gases will show a greater effect . - pubmed
VEGF mRNA decreased significantly with O ( 2 ) and O ( 2 ) + NO compared with breathing RA ( p & lt ; or = 0.05 ) . - pubmed
Lavage fluid VEGF protein and lung capillary leak rose significantly with O ( 2 ) and O ( 2 ) + NO compared with RA , but endostatin was unchanged . - pubmed
Hyperoxia and iNO suppresses lung endostatin expression , but iNO unlike hyperoxia alone does not alter lung VEGF production . - pubmed
This latter effect and that on VEGF mRNA level but not protein is abrogated by recovery in reduced F ( I ) O ( 2 ) for 72 h. - pubmed
Dual ACE and NEP inhibitors : a review of the pharmacological properties of MDL 100240 . - pubmed
MDL 100240 is a pro - drug that , upon conversion to MDL 100173 , acts as a potent dual inhibitor of ACE and NEP with a balanced activity on both enzymes . - pubmed
Studies in vivo in experimental models of hypertension and congestive heart failure confirmed the vasodilatory and natriuretic effects of MDL , which appear to be independent of the degree of activation of the renin - angiotensin - aldosterone system . - pubmed
If the promises of this novel therapeutic strategy are fulfilled , clinical trials are expected to demonstrate advantages of MDL 100240 over pure ACE inhibitors . - pubmed
In order to investigate the conditions necessary for group III mGluR autoreceptor - activation by synaptically released glutamate , we characterized the effects of selective agonists and antagonists on excitatory postsynaptic currents ( EPSCs ) evoked by several distinct PF stimulation patterns . - pubmed
Paired - pulse or four - pulse PF stimulations did not activate presynaptic group III mGluRs as revealed by the lack of effect of 1 mM MSOP on relative test EPSC amplitudes with latencies of 250 - 500 ms . - pubmed
Because GHMP kinases , thus far , have only been characterized as small molecule kinases involved in metabolic pathways , for example , amino acid and cholesterol synthesis , XOL-1 is the first member that controls nonmetabolic processes . - pubmed
Association of cannabimimetic compounds such as cannabinoids , aminoalkylindoles ( AAIs ) , and arachidonylethanolamide ( anandamide ) with the brain cannabinoid ( CB ( 1 ) ) receptor activates G - proteins and relays signals to regulate neuronal functions . - pubmed
This study aimed to examine if graft preconditioning with HO-1 could be substituted by a simple treatment with heme - degrading products such as bilirubin . - pubmed
The HO-1 - preconditioned grafts ex vivo exhibited a marked improvement of bile output and cell injury that was cancelled by blocking HO with zinc protoporphyrin - IX . - pubmed
Furthermore , a short - term rinse treatment with micromolar levels of bilirubin attenuated biliary dysfunction and cell injury of the grafts both ex vivo and in vivo even without HO-1 preconditioning . - pubmed
The protective effects of HO-1 preconditioning or bilirubin rinse appeared to involve its inhibitory effects on lipid peroxidation in hepatocytes . - pubmed
In conclusion , these results suggest that bilirubin rinse serves as a simple strategy to ameliorate hyperacute oxidative stress and hepatobiliary dysfunction of the transplanted grafts , mimicking effects of HO-1 - mediated preconditioning . - pubmed
[ Incorporation of radioactive amino acids and amylase synthesis in pancreatic tissue in vitro ] . - pubmed
The H2 concentration in the mill pot , measured by gas chromatography , increased with grinding time up to a maximum concentration of 156 ppm ( 0.35 micromol ) after 600 min . - pubmed
The protein has 1309 non - H atoms , seven S atoms , no disulfide bonds and no heavy - atom prosthetic group . - pubmed
In each case , the blood alcohol level ( BAL ) was determined every 30 minutes using about 0.5 ml whole blood obtained by venipuncture . - pubmed
However , among the male and female volunteers , oral fructose intake significantly ( p<0.05 ) increased the blood ethanol clearance rate by 66.7 and 92.3 % , decreased intoxication time by 41.7 and 40 % , reduced peak BAL by 18.3 and 0 % , but enhanced blood ethanol oxidation rate by 71.2 and 66.7 % , respectively . - pubmed
Risk factors for severe hepatic cystic disease include aging , female sex , pregnancy , use of exogenous female steroid hormones , degree of renal cystic disease , or severity of renal dysfunction ( in patients with mutations in PKD1 or PKD2 ) . - pubmed
In this paper , we propose a theoretical low - resolution 3D model for pfSHMT in complex with glycine and 5 - formyl tetrahydrofolate ( 5 - FTHF ) based on homology modeling by multiple alignment followed by intensive optimization , validation and dynamics simulations in water . - pubmed
Serum levels of lipids [ high - density lipoprotein and low - density lipoprotein cholesterol , triglycerides and lipoprotein ( a ) ] and hemostatic factors ( prothrombin , fibrinogen , plasminogen activator inhibitor-1 , and fibrin D - dimer ) were also measured . - pubmed
Furthermore , isoflavone group experienced statistically significant reductions in plasma concentrations of ICAM-1 , VCAM-1 , and E - selectin . - pubmed
Experimental heart failure caused by prolonged infusion of norepinephrine is associated with diminished endothelin induced coronary constricting effect of stimulation of ET ( A ) receptors and inversion of coronary dilating effect of stimulation of these receptors . - pubmed
At a higher concentration of p29 , the cells showed similar and accelerated morphological change , but neither externalized phosphatidylserine nor caspase-3 activation was observed . - pubmed
Gelatinases were identified on the basis of molecular weights and inhibition by EDTA . - pubmed
The 272 crossbred heifers were randomly allocated within herds to one of the four following groups : 1 ) silastic coils , 10 days treatment ( n = 65 ) ; 2 ) Norgestomet implants , 10 days treatment ( n = 71 ) ; 3 ) prostaglandin F(2alpha ) two injections 11 days apart ( n = 70 ) ; and 4 ) control ( n = 66 ) . - pubmed
Time - lapse STM studies of diastereomeric cinchona alkaloids on platinum metals . - pubmed
The adsorption of cinchonidine ( CD ) and cinchonine ( CN ) on Pt ( 111 ) and Pd ( 111 ) single crystals has been investigated by means of scanning tunneling microscopy ( STM ) in an ultrahigh vacuum system . - pubmed
The diastereomeric cinchona alkaloids , CD and CN , which are widely used as chiral modifiers of platinum group metals in catalytic enantioselective hydrogenation , showed similar adsorption modes and diffusion behavior on Pt ( 111 ) , except that the flatly adsorbed CN molecules which were free ( not in a dimer / cluster ) were significantly more mobile than their CD analogues . - pubmed
Special emphasis is put on the nonlinear effect observed in hydrogenation reactions with CD / CN mixtures . - pubmed
Our observations corroborate that this effect is mainly a consequence of the different adsorption strengths of CD and CN on Pt . - pubmed
Design of a functional membrane protein by engineering a heme - binding site in glycophorin A. - pubmed
We have designed a functional model membrane protein by engineering a bis - Histidine heme - binding site into a natural membrane protein , glycophorin A ( GpA ) , structurally characterized by the dimerization of a single transmembrane helix . - pubmed
Out of the 32 residues comprising the transmembrane helix of GpA , five amino acids were mutated ; the resulting protein , ME1 , has been characterized in dodecyl phosphocholin ( DPC ) micelles by UV - vis , CD spectroscopy , gel electrophoresis , and analytical ultracentrifugation . - pubmed
ME1 binds heme with sub - micromolar affinity and maintains the highly helical secondary structure and dimeric oligomerization state of GpA. - pubmed
The ME1 - Heme complex exhibits a redox potential of -128 + /- 2 mV vs SHE , indicating that the heme resides in a hydrophobic environment and is well shielded from the aqueous phase . - pubmed
Moreover , ME1 catalyzes the hydrogen peroxide dependent oxidation of organic substrates such as TMB ( 2,2 ' , 5,5 '- tetramethyl - benzidine ) . - pubmed
Ketamine in post - tonsillectomy pain . - pubmed
Isoprenoids are synthesized through the condensation of five - carbon intermediates , isopentenyl diphosphate ( IPP ) and dimethylallyl diphosphate ( DMAPP ) , derived from two distinct biosynthetic routes : cytosolic mevalonate ( MVA ) and plastidial 2 - C - methyl - D : - erythritol 4 - phosphate ( MEP ) pathways . - pubmed
biloba embryo , the transcripts of GbIDS2 predominantly occurred in the embryo roots , where diterpene ginkgolides are biosynthesized . - pubmed
Levels of PtIDS2 transcripts in the diterpenoid resin - producing wood were 4 - 5 times higher than those in other tissues . - pubmed
Higher levels of GbIDS1 transcripts were induced by light , whereas those of GbIDS2 were increased by methyl jasmonate treatment . - pubmed
Unphosphorylated RIIalpha ( 90 - 400 ) , a deletion mutant , undergoes major conformational changes as both of the cAMP - binding domains wrap around the C subunit 's large lobe . - pubmed
This structure demonstrates the conserved and isoform - specific features of RI and RII and the importance of ATP , and also provides a new paradigm for designing isoform - specific activators or antagonists for PKA . - pubmed
Remarkably , insecticidal and hemolytic activities were both dependent on a common , well defined cluster of hydrophilic residues on one face of the cyclotide . - pubmed
We investigated whether 4 weeks of near normalisation of blood glucose ( BG ) improves the potentiation of glucose - stimulated insulin secretion by GLP-1 . - pubmed
Beta cell function was investigated before and after insulin treatment using stepwise glucose infusions and infusion of saline or GLP-1 ( 1.0 pmol kg(-1 ) min(-1 ) ) , resulting in supraphysiological total GLP-1 concentrations of approximately 200 pmol / l. The responsiveness to glucose or glucose+GLP-1 was expressed as the slope of the linear regression line relating insulin secretion rate ( ISR ) and plasma glucose concentration ( pmol kg(-1 ) min(-1 ) [ mmol / l](-1 ) ) . - pubmed
In contrast , near normalisation of blood glucose improved beta cell sensitivity to glucose during glucose+GLP-1 infusion ( 1.27+/-0.2 before vs 1.73+/-0.31 after ; p<0.01 ) . - pubmed
Analysis of RSpo deletion mutants indicates that the cysteine - rich furin domains are sufficient and essential for the amplification of Wnt signaling and inhibition of DKK1 , suggesting that Wnt amplification by RSpo proteins may be a direct consequence of DKK1 inhibition . - pubmed
Modulation of peripheral serotonin levels by novel tryptophan hydroxylase inhibitors for the potential treatment of functional gastrointestinal disorders . - pubmed
Kinase inhibitor studies with macrophages demonstrated that the Src family PTKs were required for this tyrosine phosphorylation activity . - pubmed
In silico methods and site - directed mutagenesis were used to identify EPIYG and EPVYA motifs in LspA1 that contained tyrosines that were targets for phosphorylation . - pubmed
A total of four tyrosines could be phosphorylated in LspA1 , with LspA2 containing eight predicted tyrosine phosphorylation motifs . - pubmed
Macrophage lysates could also tyrosine phosphorylate the LspA proteins and an LspA1 fusion protein via a mechanism that was dependent on the presence of both divalent cations and ATP . - pubmed
Nicotine exposure also resulted in significantly reduced pancreatic respiratory chain enzyme activity , degranulation of beta cells , elevated islet oxidative stress and impaired glucose - stimulated insulin secretion compared to saline controls at 26 weeks of age . - pubmed
This paper demonstrated the selective determination of folic acid ( FA ) in the presence of important physiological interferents , ascorbic acid ( AA ) and uric acid ( UA ) at physiological pH using electropolymerized film of 5 - amino-2 - mercapto-1,3,4 - thiadiazole ( p - AMT ) modified glassy carbon ( GC ) electrode . - pubmed
However , the p - AMT film modified electrode not only separates the voltammetric signals of AA , UA and FA with potential differences of 170 and 410 mV between AA - UA and UA - FA , respectively but also shows higher oxidation current for these analytes . - pubmed
Magnetoreception through cryptochrome may involve superoxide . - pubmed
Accordingly , we investigate here a possible radical pair - based reaction in the photoreceptor cryptochrome that reduces the protein 's flavin group from its signaling state FADH * to the inactive state FADH- ( which reacts to the likewise inactive FAD ) by means of the superoxide radical , O2 * - . - pubmed
To define group determinants of recognition , we used a model composed of a peptide analogue of the alpha4 helix , oligonucleotides mimicking processed and unprocessed U5 LTR end and 5 mM Mg ( 2 + ) . - pubmed
Proton chemical shift deviations showed that specific binding need conserved amino acids in the alpha4 helix and conserved nucleotide bases and backbone groups at LTR ends . - pubmed
Impaired S - phase arrest in acute myeloid leukemia cells with a FLT3 internal tandem duplication treated with clofarabine . - pubmed
We show defects in the response of mutant FLT3 AML cells to the S - phase drug clofarabine that could account for the apparent contradiction . - pubmed
Furthermore , cdc25A message levels are maintained by the FLT3 - ITD , such that message is reduced by 87.5 % on exposure to FLT3 small interfering RNA . - pubmed
Primary FLT3 - ITD samples from untreated patients also display impaired cell cycle arrest and show enhanced sensitivity on prolonged treatment with clofarabine compared with wild - type samples . - pubmed
CONCLUSION : There is a reversal of phenotype in mutant FLT3 cells dependent on the length of exposure to clofarabine . - pubmed
The neurodegenerative changes progressed with age and were accompanied by up - regulation of the neuronal tissue - type plasminogen activator , a serine protease known to mediate microglial activation . - pubmed
In addition , a very active esterifying system , capable of utilizing either ( 3 ) H - retinol or ( 3 ) H - retinol bound to cellular retinol binding protein as substrates , has been demonstrated in the microsomal fraction of the cell . - pubmed
Furthermore , spinasterol suppressed the lipopolysaccharide ( LPS)-induced expression of pro - inflammatory enzymes and inflammatory mediators in BV2 microglia . - pubmed
Spinasterol also suppressed the production of nitric oxide ( NO ) , prostaglandin E2 ( PGE2 ) , tumor necrosis factor - alpha ( TNF - alpha ) , and interleukin-1beta ( IL-1beta ) through extracellular signal - regulated kinase ( ERK ) pathway - dependent expression of HO-1 . - pubmed
Tryptophan fluorescence of the MaSp - NTDs reveals a change in conformation when pH is decreased , and the pH at which the transition occurs is determined by the amount and type of salt present . - pubmed
In the current report , we investigated the potency of DBM , DBP , and DBA in relation to curcumin for their ability to suppress TNF - induced NF - kappaB activation , NF - kappaB - regulated gene products , and cell proliferation . - pubmed
We found that all four agents were active in suppressing NF - kappaB activation ; curcumin was most active and DBM was least active . - pubmed
When examined for its ability to inhibit the direct DNA binding activity of p65 , a subunit of NF - kappaB , only DBP inhibited the binding . - pubmed
For inhibition of TNF - induced IKK activation , DBA was most active . - pubmed
For suppression of TNF - induced expression of NF - kappaB - regulated gene products such as COX-2 ( inflammation marker ) , cyclin D1 ( proliferation marker ) , and VEGF ( angiogenesis marker ) , DBA and curcumin were more active than DBM . - pubmed
Overall , our results indicate that although curcumin and its analogues exhibit activities to suppress inflammatory pathways and cellular proliferation , a lack of Michael acceptor units in DBM and DBP can reduce their activities . - pubmed
Connexin-43 hemichannels mediate cyclic ADP - ribose generation and its Ca2 + -mobilizing activity by NAD+/cyclic ADP - ribose transport . - pubmed
The ADP - ribosyl cyclase CD38 whose catalytic domain resides in outside of the cell surface produces the second messenger cyclic ADP - ribose ( cADPR ) from NAD (+ ) . - pubmed
cADPR increases intracellular Ca ( 2 + ) through the intracellular ryanodine receptor / Ca ( 2 + ) release channel ( RyR ) . - pubmed
It has been known that intracellular NAD (+ ) approaches ecto - CD38 via its export by connexin ( Cx43 ) hemichannels , a component of gap junctions . - pubmed
However , it is unclear how cADPR extracellularly generated by ecto - CD38 approaches intracellular RyR although CD38 itself or nucleoside transporter has been proposed to import cADPR . - pubmed
Moreover , it has been unknown what physiological stimulation can trigger Cx43 - mediated export of NAD (+ ) . - pubmed
Here we demonstrate that Cx43 hemichannels , but not CD38 , import cADPR to increase intracellular calcium through RyR. - pubmed
Protein kinase A ( PKA ) activation also induced calcium mobilization in the same way as FcgammaR stimulation . - pubmed
FcgammaR stimulation - induced calcium mobilization was blocked by PKA inhibition , indicating that PKA is a linker between FcgammaR stimulation and NAD(+)/cADPR transport . - pubmed
Cx43 overexpression in Cx43 - negative cells conferred extracellular cADPR - induced calcium mobilization by the mediation of cADPR import . - pubmed
Our data suggest that Cx43 has a dual function exporting NAD (+ ) and importing cADPR into the cell to activate intracellular calcium mobilization . - pubmed
In this study , the amino acid sequences of kappa - conotoxin RIIIJ and its four mutageneses were converted into ten HP sequences according to the normalized hydrophobicity index . - pubmed
The contribution of endothelium - derived hyperpolarizing factor ( EDHF ) to endothelium - dependent relaxation was impaired in uremic arteries after stimulation with bradykinin , but not acetylcholine , reflecting the agonist - specific differences . - pubmed
TP was administered as a continuous intravenous dose of 1.3 mug / kg / h and dopamine was administered in doses up to 20 mug / kg / min to maintain a mean arterial pressure of 70 + - 5 mm Hg for 48 h. Hemodynamic changes , ratio of partial pressure of arterial oxygen to fraction of inspired oxygen ( PaO ( 2 )/ FiO ( 2 ) ) , and VEGF were recorded prospectively . - pubmed
VEGF concentrations significantly decreased from baseline levels in the TP group ( p & lt ; 0.05 ) at 48 h ; there was no difference in the dopamine group ( p & gt ; 0.05 ) at 48 h vs. baseline . - pubmed
Our aim was to compare the effects of oral mycophenolate mofetil ( MMF ) with low - dose prednisone and the full - dose corticosteroids ( CS ; prednisone ) for the induction therapy of HSPN with large proteinuria . - pubmed
RESULTS : At 6 months , the estimated glomerular filtration rate level remained stable , while the urine protein decreased significantly in both groups , and the remission rate was 76.9 % in the CS group and 55.5 % in the MMF group ( p = 0.101 ) . - pubmed
With a median follow - up of 28.8 months in the CS group and 28.2 months in the MMF group , the overall remission rate was 80.8 % in the CS group and 77.8 % in the MMF group ( p = 0.788 ) . - pubmed
The MMF group had less side effects than the CS group ( 48.1 vs. 76.9 % , p = 0.031 ) . - pubmed
The relapse was 4/21 ( 19.0 % ) in the CS group and 0/21 in the MMF group ( p = 0.115 ) . - pubmed
rhizogenes strain A13 , when cultured in optimized half - strength MS medium containing 1.5 times the standard amount of ammonium nitrate and potassium nitrate and 5 % ( w / v ) sucrose , had exhibited improvement in growth index , that is , the fresh biomass was almost double as compared to its initial growth in unmodified half - strength MS medium . - pubmed
A predominance of alpha - helix structures was detected by circular dichroism with thermal stability up to 45 C. The oligomeric assemblage of SELA was investigated by glutaraldehyde crosslinking experiments indicate that SELA homodecameric structure is the result of a stepwise addition of intermediate oligomeric states and not a direct monomer to homodecamer transition . - pubmed
HER2 Status by Standardized Immunohistochemistry and Silver - Enhanced In Situ Hybridization in Korean Breast Cancer . - pubmed
We investigated the HER2 status in Korean breast cancer by immunohistochemistry ( IHC ) and silver - enhanced in situ hybridization ( SISH ) , as the first step toward building a nationwide quality assurance program for HER2 testing . - pubmed
Pectinase was entrapped in polyvinyl alcohol ( PVA ) sponge and the optimum pH and temperature of the free and immobilized enzymes were shifted from 4 , 40 C to 6 , 50 C respectively . - pubmed
Vesicular nucleotide transporter regulates the nucleotide content in airway epithelial mucin granules . - pubmed
Nucleotides within the airway surface liquid promote fluid secretion via activation of airway epithelial purinergic receptors . - pubmed
We assessed the contribution of the recently identified SLC17A9 vesicle nucleotide transporter ( VNUT ) to the nucleotide availability within isolated mucin granules and further examined the involvement of VNUT in mucin granule secretion - associated nucleotide release . - pubmed
Mucin granules isolated from VNUT short hairpin RNA - expressing cells exhibited a marked reduction of ATP , ADP , AMP , and UTP levels within granules . - pubmed
These results suggest that VNUT is the relevant nucleotide transporter responsible for the uptake of cytosolic nucleotides into mucin granules . - pubmed
By controlling the entry of nucleotides into mucin granules , VNUT contributes to the release of purinergic signaling molecules necessary for the proper hydration of co - released mucins . - pubmed
RESULTS : Nicotine abstinence induced a decrease in heart rate and systolic blood pressure ( BP ) before the BH procedure ( heart rate : 78.80 + - 11.43 under nicotine , 70.88 + - 10.83 under placebo ; systolic BP : 124.90 + - 11.34 under nicotine , 121.18 + - 13.44 under placebo ) and shorter BH duration relative to the nicotine patch condition . - pubmed
Silibinin meglumine , a water - soluble form of milk thistle silymarin , is an orally active anti - cancer agent that impedes the epithelial - to - mesenchymal transition ( EMT ) in EGFR - mutant non - small - cell lung carcinoma cells . - pubmed
As a single agent , oral silibinin meglumine notably decreased the overall volumes of NSCLC tumors as efficiently as did the EGFR tyrosine kinase inhibitor ( TKI ) gefitinib . - pubmed
Because the epithelial - to - mesenchymal transition ( EMT ) is required by a multiplicity of mechanisms of resistance to EGFR TKIs , we evaluated the ability of silibinin meglumine to impede the EMT in vitro and in vivo . - pubmed
Lincomycin biosynthesis involves a tyrosine hydroxylating heme protein of an unusual enzyme family . - pubmed
Mass spectrometry and Soret band - excited Raman spectroscopy of LmbB2 showed that LmbB2 contains heme b as prosthetic group . - pubmed
The CO - reduced differential absorption spectra of LmbB2 showed that the coordination of Fe was different from that of cytochrome P450 enzymes . - pubmed
LmbB2 exhibits sequence similarity to Orf13 of the anthramycin biosynthetic gene cluster , which has recently been classified as a heme peroxidase . - pubmed
Tyrosine hydroxylating activity of LmbB2 yielding DOPA in the presence of ( 6R )- 5,6,7,8 - tetrahydro - L - biopterin ( BH4 ) was also observed . - pubmed
Synthesis and biological evaluation of novel tetrahydro - beta - carboline derivatives as antitumor growth and metastasis agents through inhibiting the transforming growth factor - beta signaling pathway . - pubmed
Here we demonstrated the identification of a series of tetrahydro - beta - carboline derivatives from virtual screening which potentially inhibit the TGFbeta signaling pathway . - pubmed
Optimization of the initial hit compound 2 - benzoyl-1,3,4,9 - tetrahydro - beta - carboline ( 8a ) through substitution at different positions to define the structure - activity relationship resulted in the discovery of potent inhibitors of the TGFbeta signaling pathway . - pubmed
Increased CEH expression in the liver significantly increased the flux of HDL - CE to bile acids . - pubmed
In the LDLR(-/- ) background , this enhanced elimination of cholesterol led to attenuation of diet - induced atherosclerosis with a consistent increase in fecal sterol secretion primarily as bile acids . - pubmed
Taken together with the observed reduction in atherosclerosis by increasing macrophage CEH - mediated cholesterol efflux , these studies establish CEH as an important regulator in enhancing cholesterol elimination and also as an anti - atherogenic target . - pubmed
As a result of their sponge - like morphology , aluminum phosphonates adsorb conductive small molecules such as phosphonic acids , which results in a very high proton conductivity of approximately 5 x 10(-2 ) S cm(-1 ) at 120 C and 50 % relative humidity ( RH ) . - pubmed
The results showed an opposite behaviour among ozone and NO and NO2 concentrations in urban and suburban zones , marked by maximums of ozone ( minimums NO ( x ) ) in spring and summer and minimums ( maximums ) in autumn and winter . - pubmed
During the observation period , the proportion of pain - free attacks at 2 h did not significantly differ between frovatriptan and the comparators in either men ( 32 vs. 38 % , p = NS ) or women ( 30 vs. 33 % , p = NS ) . - pubmed
coli on several carbon sources is dependent on the catabolite repressor / activator ( Cra ) protein although a Cra consensus DNA - binding site is not present in the control regions of the relevant catabolic operons . - pubmed
Apocynin is a selective inhibitor of the phagocyte NADPH oxidase Nox2 that can be applied orally and is remarkably effective at low dose . - pubmed
The structural optimization and normal mode analysis were performed for model compound C , consisting of thiophosphoryl core , one branch with three repeated units , and four 4 - oxybenzaldehyde terminal groups on the basis of the density functional theory ( DFT ) at the PBE / TZ2P level . - pubmed
The first one - pot synthesis of metal - organic frameworks functionalised with two transition - metal complexes . - pubmed
The synthesis of a metal - organic framework ( UiO-67 ) functionalised simultaneously with two different transition metal complexes ( Ir and Pd or Rh ) through a one - pot procedure is reported for the first time . - pubmed
BACKGROUND : Safety and efficacy of tenofovir disoproxil fumarate ( TDF ) as a component of antiretroviral therapy ( ART ) have been demonstrated in clinical trials . - pubmed
This meta - analysis explored the frequency of discontinuation attributed to renal adverse events ( AEs ) in randomized , controlled clinical studies that used TDF - containing regimens for ART - naive , HIV - infected patients . - pubmed
Phylogenetic trees based on the amino acid and nucleotide sequences of eIF-4A demonstrated a similar topology with the classical systematics , suggesting that it has the potential value in phylogenetic inference of eukaryotes . - pubmed
T - cell cytokine gene polymorphisms and vitamin D pathway gene polymorphisms in end - stage renal disease due to type 2 diabetes mellitus nephropathy : comparisons with health status and other main causes of end - stage renal disease . - pubmed
BACKGROUND : T - cell cytokine gene polymorphisms and vitamin D pathway gene polymorphisms were evaluated as possibly associated with end - stage renal disease ( ESRD ) resulting from type 2 diabetes mellitus ( DM ) nephropathy . - pubmed
A frequency distribution of T - cell - related interleukin ( IL ) genes ( IL18 rs360719 , IL12A rs568408 , IL12B rs3212227 , IL4R rs1805015 , IL13 rs20541 , IL28B rs8099917 , IL28B , and rs12979860 ) and vitamin D pathway genes ( GC genes : rs2298849 , rs7041 , and rs1155563 ; VDR genes : rs2228570 , rs1544410 ; and RXRA genes : rs10776909 , rs10881578 , and rs749759 ) was compared between groups . - pubmed
There was an overexpression of inflammatory cytokines in the mother , such as IL-6 and TNF - alpha , and , in addition , PGE2 was also increased . - pubmed
Neonates showed lower levels of IL-6 and TNF - alpha and higher values of sTNF - RII and PGE2 in comparison with their mothers . - pubmed
On the basis of quantum chemical calculations , these bands are assigned to a C2 isomer of ( OCS ) 2 ( + ) , in which an intermolecular semi - covalent bond is formed between the sulfur ends of the two OCS components by the charge resonance interaction , and the positive charge is delocalized over the dimer . - pubmed
In addition to the bands of the OCS (- ) core isomer , we found another band at ~1740 cm(-1 ) , which can be assigned to isomers having an ( OCS ) 2 (- ) ion core ; this dimer core has C2 symmetry and ( 2)A electronic state . - pubmed
TTF was significantly longer in GXR versus placebo ( p = 0.003 ) . - pubmed
Notably , we did not detect the assumed GSP - mechanisms driving these changes : despite an increased metabolic rate there was neither an increase of C - rich biomolecules ( instead fat and sugar contents decreased under predation risk ) , nor a decrease of N - rich proteins . - pubmed
The concentration of 17beta - estradiol ( E2 ) was measured through stages of wastewater treatment at a central Illinois wastewater treatment plant ( WWTP ) . - pubmed
The concentration of E2 was compared with demographic effects of a university ; physical parameters of the wastewater ( dissolved oxygen , pH , and temperature ) ; and daily influent and effluent flow rates . - pubmed
The molecular modeling of PHY US42 indicated that this phytase belongs to the group of beta - propeller phytases that are usually calcium - dependent . - pubmed
Its activity was calcium - dependent and maximal at pH 7 and 65 C. The enzyme was perfectly stable at pH ranging from 5 to 10 and its thermostability was greatly enhanced in the presence of calcium . - pubmed
Indeed , PHY US42 maintained 80 % of activity after 10 min of incubation at 75 C in the presence of 5 mM CaCl2 . - pubmed
Compared with never users , the pooled RRs of PLC were 0.60 ( 95 % CI 0.37 , 0.96 ) for ever users of MHT , 0.73 ( 95 % CI 0.46 , 1.17 ) for ever users of estrogen - only therapy ( ET ) and 0.67 ( 95 % CI 0.45 , 1.02 ) for ever users of estrogen - progestin therapy ( EPT ) . - pubmed
Also 25 hydroxyvitaminD ( 25 - OH - VitD ) , parathyroid hormone ( iPTH ) , posphorus , serum uric acid , renal resistive index ( RRI ) and C - reactive protein ( CRP ) showed a significant difference between the two groups ( p=0.002 , p<0.001 , p<0.001 , p<0.001 , p<0.001 , p<0.001 ) . - pubmed
Paracetamol was the most common drug used for self - medication 31 % , followed by Azithromycin 17.6 % and combination of Paracetamol and Ibuprofen 15.6 % , Cetirizine 8.6 % , Amoxicillin 6.5 % , Omeprazole 6.3 % , Albendazole 3.3 % , Mefenemic acid 2.8 % , Cefpodoxime2 % respectively . - pubmed
Here , a focused metabolomics approach was used to identify changes in folate catabolism as well as the S - adenosylmethionine ( SAM ) cycle following NAT1 knockdown with shRNA . - pubmed
By contrast , the flux of homocysteine in the medium was different following NAT1 knockdown after the methionine content was exhausted suggesting a need for this metabolite in methionine synthesis . - pubmed
RESULTS : The inflection point of serum 25 - OHD level for maximal suppression of PTH was at 18.0 ng / mL ( 95 % confidence interval , 14.3 - 21.7 ng / mL ) . - pubmed
Gamma - hydroxybutyrate ( GHB ) is a GHB -/ GABA - B receptor agonist inducing a broad spectrum of subjective effects including euphoria , disinhibition , and enhanced vitality . - pubmed
A whole - brain analysis showed increased rCBF in the bilateral anterior cingulate cortex ( ACC ) and the right anterior insula under GHB ( p<0.05 , cluster - corrected ) . - pubmed
Interaction analyses revealed that GHB - induced increase of body awareness was accompanied by increased rCBF in ACC , whereas relaxation under GHB was accompanied by elevated rCBF in right anterior insula ( p<0.05 , uncorrected ) . - pubmed
In conclusion , enhancement of emotion and body awareness , and increased perfusion of insula and ACC bears implications both for the properties of GHB as a drug of abuse as well as for its putative personalized potential for specific therapeutic indications in affective disorders . - pubmed
Inhibition of cell proliferation through an ATP - responsive co - delivery system of doxorubicin and Bcl-2 siRNA . - pubmed
Herein , DNA duplex was constructed through the hybridization of adenosine triphosphate ( ATP)-responsive aptamer and its cDNA in which GC - rich motif could be used to load doxorubicin ( DOX ) , and then , cationic polymer PEI25 K was used as a carrier to simultaneously condense DOX - Duplex and Bcl-2 siRNA to prepare the ternary nanocomplex polyethylenimine ( PEI)/DOX - Duplex / siRNA . - pubmed
Overall , the ATP - responsive nanocarrier for co - delivering DOX and Bcl-2 siRNA has been demonstrated to be a smart delivery system with favorable anti - proliferative effect , especially for solving the multidrug resistance of tumors . - pubmed
The bulk filtered phosphate is reabsorbed in the renal proximal tubule by two specialized phosphate transporters , NaPi - IIa and NaPi - IIc . - pubmed
Phosphate balance is regulated by dietary phosphate intake , and by the action of the parathyroid hormone , vitamin D3 and fibroblast growth factor-23 ( FGF-23 ) . - pubmed
All these regulators exert their effect by modulating the activity of the proximal - tubular phosphate transporters , NaPi - IIa and NaPi - IIc . - pubmed
Arbutin ( p - hydroxyphenyl - beta - D - glucopyranoside ) , a well - known tyrosinase inhibitor , has been widely used as a cosmetic whitening agent . - pubmed
The underlying molecular mechanism for these effects involves decreased levels of reactive oxygen species ( ROS ) , improvement of daf-16 nuclear localization , and up - regulated expression of daf-16 and its downstream targets , including sod-3 and hsp16.2 . - pubmed
Though not natural minerals themselves , both compounds adopt the pavonite structure , which crystallizes in the C2 / m space group and consists of two connected , alternating defect rock - salt slabs of varying thicknesses to create a three - dimensional lattice . - pubmed
15 - hydroxyprostaglandin dehydrogenase ( 15 - PGDH ) is a prostaglandin metabolizing enzyme that oxidizes the hydroxyl group at carbon 15 ( C15 ) . - pubmed
The aim of the present work is to propose the main amino acids that catalyze the reaction through studying the intermolecular interaction between the ligand and the enzyme inside the active site using molecular dynamics simulation ( MD ) . - pubmed
Therefore , MD simulations for two 15 - PGDH systems bound with a substrate ( PGE2 ) or an inhibitor ( compound 4 ) were performed to investigate the importance of ligand interaction on the behavior of amino acids in the active site . - pubmed
Among the emerging new psychoactive substances ( NPS ) , compounds carrying an N - ortho - methoxybenzyl substituent , the so - called NBOMes , represented a highly potent group of new hallucinogens . - pubmed
Bioanalytical method validation as per EMA guidelines was carried out where the proposed method revealed linearity over the concentration range of 5 - 5000 and 10 - 1500 ng mL-1 for sofosbuvir and velpatasvir , respectively . - pubmed
The ratio of 40K/232Th and 40 K /238U were calculated , which indicates the presence of 5.79 and 2.69 times more K - bearing minerals as compared with Th and U - bearing minerals in the soil samples , respectively . - pubmed
For that , primary cortical cultures exposed to oxygen and glucose deprivation ( OGD ) were used as a model . - pubmed
In this study , we also demonstrate that selective activation of GPER induced astrocyte apoptosis via the phospholipase C pathway and subsequent intracellular calcium rise , whereas in neurons , this effect was not observed . - pubmed
The enzymatic determination of glucose in soft drinks , based on the glucose concentration measurement by means of the coupled reactions of glucose oxidase and peroxidase , has been implemented at the University of Malaga and optimized according to the Biochemistry and Chemistry undergraduate students ' results and feedback throughout the last few years . - pubmed
Two identical CAD / CAM titanium abutments only differing in color ( gray and pink ) were fabricated along with an all - ceramic zirconia crown . - pubmed
Under AM 1.5 illumination ( 100 mW / cm2 ) conditions , the optimized PCBM : P3HT solar cells with a chemical bath deposited a CdS layer of thickness 70 nm and showed 50 % power conversion efficiency enhancement , in comparison with similar solar cells with optimized dense TiO2 of 50 nm thickness prepared by spray pyrolysis . - pubmed
Conclusion : These data suggested that female sexual steroids have a therapeutic effect on gastrointestinal ischemic disorders by reduction of MPO and gastric acid , and increasing gastric GSH & amp ; SOD levels following gastric IR . - pubmed
Triiodothyronine ( T3 ) and estrogen ( E2 ) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor - kappa beta ( RANKL ) and osteoprotegerin ( OPG ) expressed by osteoblasts . - pubmed
Glutathione S - transferase mu ( GSTM ) belongs to a family of phase II detoxification enzymes that catalyze the conjugation of reduced glutathione ( GSH ) to a wide range of endogenous and exogenous electrophilic compounds . - pubmed
MTS assays , Transwell assays , and flow cytometry were used to explore the function of GSTM1 in vitro . - pubmed
In vitro , Transwell and doxorubicin ( DOX)-induced apoptosis assays revealed that GSTM1 showed opposite functions in different HCC cell lines with varied TP53 genotype statuses . - pubmed
The overexpression of GSTM1 in the above cell lines led to a significant decrease in ROS and an increase in GSH concentration and TP53 levels , suggesting that the controversial role of GSTM1 resulted from the TP53 genotype of HCC cells . - pubmed
RecIL-37 treatment increased endurance exercise capacity by 2.4 - fold , which was accompanied by a 2.9 - fold increase in the phosphorylated AMP - activated kinase ( AMPK ) to AMPK ratio ( i.e. - pubmed
RecIL-37 treatment also improved whole - body insulin sensitivity and glucose tolerance ( p & lt ; .05 vs. vehicle ) . - pubmed
Plasma metabolomics analysis revealed that recIL-37 treatment altered metabolites related to pathways involved in NO synthesis ( e.g. - pubmed
Nor1 , the third member of the Nr4a subfamily of nuclear receptor , is garnering increased interest in view of its role in the regulation of glucose homeostasis . - pubmed
Mitochondrial localization of Nor1 reduced glucose oxidation , lowered ATP production rates , and inhibited glucose - stimulated insulin secretion . - pubmed
Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads ( pGC ) with honeycomb - biomimetic microchannels based on graphene oxide ( GO ) and chitosan ( CS ) . - pubmed
Overexpression of Sox4 promoted cardiomyocyte apoptosis with or without H2 O2 , whereas knocking down of Sox4 alleviated H2 O2 -induced apoptosis in cardiomyocytes . - pubmed
Mechanistically , there is a potential Sox4 - binding site in the promoter region of Bim , and forced expression of Sox4 significantly promoted Bim expression in cultured cardiomyocytes with or without H2 O2 , whereas knocking down of Sox4 inhibited the expression of Bim . - pubmed
Positive allosteric modulators ( PAMs ) of GABAB receptor represent an interesting alternative to receptor agonists such as baclofen , as they act on the receptor in a more physiological way and thus are devoid of the side effects typically exerted by the agonists . - pubmed
Based on our interest in the identification of new GABAB receptor PAMs , we followed a merging approach to design new chemotypes starting from selected active compounds , such as GS39783 , rac - BHFF , and BHF177 , and we ended up with the synthesis of four different classes of compounds . - pubmed
CONCLUSIONS : The BPA introduced in our DKA management algorithm successfully reduced total time on insulin and the incidence of hypoglycemia . - pubmed
Relationship between amyloid and tau levels and its impact on tau spreading . - pubmed
Positive reactions of 3beta - steroid - dehydrogenase and enzymes of the pentose phosphate cycle indicate steroid hormone production ; the pathways of the steroid synthesis , however , are probably different in adult and immature testes . - pubmed
What is the clinical importance of alterations of the hemoglobin oxygen affinity in preserved blood -- especially as produced by variations of red cell 2,3 DPG content ? - pubmed
The antibody against hepatitis B surface antigen ( anti - HBs ) content of two of the administered batches of immune human serum globulin was 1:32 and 1:64 . - pubmed
None of the babies became hepatitis B surface antigen carriers , and anti - HBs developed without obvious clinical hepatitis in one baby . - pubmed
Conventional immune human serum globulin in larger doses may be a relatively safe and effective prophylaxis against the development of hepatitis B surface antigen carrier state even if the anti - HBs content in the administered dose is relatively small . - pubmed
The effects of four calcium channel blockers ( nicardipine , nifedipine , verapamil , and diltiazem ) on free radical injury in cultured endothelial cells were studied and compared with those of butylated hydroxytoluene . - pubmed
SP and capsaicin , but not MC increased the concentration of FITC - albumin in the nasal fluid . - pubmed
Scintigraphy with 99mTc ( V )- DMSA demonstrated uptake in medullary carcinoma of the thyroid gland ( MCT ) . - pubmed
Iodine-131 metaiodobenzylguanidine ( MIBG ) scintigraphy showed the bilateral pheochromocytomas but did not demonstrate uptake in the MCT . - pubmed
Successful execution of the procedure requires attention to technical details such as the fixation of tissue , use of proteolytic enzymes such as trypsin for formalin - fixed tissues , and the choice of the appropriate antibody and controls . - pubmed
Large tumours are rich in necrotic cells , and cathepsin D and serine - proteases are the main hydrolytic enzymes involved in the proteolytic action on melanosomes . - pubmed
Basic hemodynamic parameters and the state of the renin - aldosterone system were determined . - pubmed
We purified NF - kappa B over 50,000 - fold and identified two protein species , 42 and 44 kDa , that could be eluted and renatured from a sodium dodecyl sulfate / polyacrylamide gel to give specific DNA - binding activity . - pubmed
One of these new derivatives , FVIII delta II , in which amino acids 771(pro)-1666(asp ) have been deleted , no longer contains the protease cleavage site at amino acid position 1648 ( arg )- 1649 ( glu ) known to be involved in the initial step of FVIII processing . - pubmed
FVIII delta II has the following properties : ( i ) it exhibits FVIII procoagulant activity ; ( ii ) it is expressed at 5 - fold higher levels than is the complete molecule in comparable systems ; ( iii ) it migrates for the most part as a single major band on SDS - PAGE , in contrast to the complete molecule ; ( iv ) it is activated to a greater extent by thrombin than is either rFVIII or pdFVIII ; and ( v ) it retains the ability to bind von Willebrand factor ( vWf ) . - pubmed
The time course of vesicle volume is determined by the self - quenching of entrapped fluorescein sulfonate ( FS ) , the best of a series of dyes screened for self - quenching , brightness , and vesicle loading / trapping . - pubmed
Urea Ps was 2 x 10(-6 ) cm / s with sigma 0.95 - 1.00 on the basis of the fluorescence time course analysis and the extravesicular [ urea ] required to obtain zero initial volume flow ( null method ) when vesicles were loaded with sucrose.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
[ Correlation of prolactin , sodium , potassium and calcium in the amniotic fluid , in the mother and in the newborn infant ] . - pubmed
An analog of ACTH / MSH ( 4 - 9 ) , ORG-2766 , reduces cerebral uptake of morphine . - pubmed
The results explain , in part , how some fragments and analogs of ACTH / MSH may antagonize behavioral actions of morphine , even though some of these peptides lack significant opiate receptor binding properties . - pubmed
In the fractured face of dispersions composed of egg yolk phosphatidylcholine and glucosyl dipalmitylglycerol , particles having a diameter of 3 - 6 nm , instead of tubular structures , were observed , suggesting that the occurrence of tubular structure may depend on the fatty acid compositions of the mixtures . - pubmed
A mixture of dipalmitoylphosphatidylcholine and galactosylceramide did not show any distinct tubular or globular structures on the fractured face . - pubmed
Leukocyte interferons eluting predominantly in 0.5 M NaCl buffer , 2 . - pubmed
Lymphoblastoid interferons eluting mostly in 1 M NaCl buffer , and 3 . - pubmed
Cell culture - derived fibroblast and bladder carcinoma interferons requiring ethylene glycol in addition to 1 M NaCl for elution . - pubmed
Desorption behavior from Blue Sepharose did not necessarily correlate with the presence of specific antigenic markers and no consistent segregation of Le and F antigens in individual fractions was observed . - pubmed
Direct sequencing of the PLP gene and PLP mRNAs from the brain of the PMD patient revealed a G to T transition in exon V of the PLP gene , which leads to a glycine to cysteine substitution at residue 220 . - pubmed
AFP has been shown to bind various ligands in vitro including fatty acids , estrogens , thyroid hormones and retinoic acids . - pubmed
The presence of nine such hydrophobic repeats in the third domain of the AFP molecule mimics the heptad dimerization repeats found in the retinoic acid , thyroid , c - erbA and other members of the nuclear receptor superfamily . - pubmed
Computer analysis revealed that the most conservative matching occurred between AFP and the retinoic acid class of receptors . - pubmed
Hypocalcemia and an elevation in circulating 1,25 ( OH ) 2D3 in ex ovo embryos were observed by 15 days . - pubmed
Calcium and phosphorus concentrations in femora and tibiae in ex ovo embryos were significantly lower compared to their normal counterparts . - pubmed
The CRT group exhibited a " flattened " hypotensive portion of the carotid sinus - heart rate baroreflex curve , but this appeared to be due to the increased neck muscle mass of the subjects . - pubmed
Characteristics of the inhibition of NADPH oxidase activation in neutrophils by apocynin , a methoxy - substituted catechol . - pubmed
Phagocytes are able to generate reactive oxygen species by an activatable NADPH oxidase system . - pubmed
We investigated the inhibition of NADPH oxidase activation by a methoxy - substituted catechol , apocynin . - pubmed
The lagtime of effect of apocynin in neutrophils relatively deficient of myeloperoxidase was about 50 % longer when compared with normal cells . - pubmed
Immunoblots of neutrophil membranes , isolated at 2 and 7 min after STZ stimulation of neutrophils , demonstrated translocation of the cytosolic oxidase components p47 - phox and p67 - phox to the membrane fraction . - pubmed
These properties suggest that apocynin is an intracellular inhibitor of the assembly of NADPH oxidase in neutrophils and eosinophils and that apocynin requires conversion by peroxidases to exert its inhibitory effect . - pubmed
The potential therapeutic value of apocynin was demonstrated in vitro by its ability to protect secretory leukocyte proteinase inhibitor from oxidative inactivation by neutrophils . - pubmed
The cell surface antigen has an amino acid sequence homologous to Aplysia ADP - ribosyl cyclase that catalyzes the conversion of NAD to cyclic ADP - ribose with a calcium - mobilizing activity . - pubmed
A putative hyaluronate ( HA)-binding motif which has recently been identified in CD44 antigen existed in the extracellular domain and intracellular amino terminus of CD38 antigen . - pubmed
Effects of culture with unsaturated fatty acids on lymphocyte protein kinase activities . - pubmed
Substance P and vasopressin levels in the perfusate were the same in controls and after sympathectomy . - pubmed
He remained insulin - independent , with normal fasting blood glucose and hemoglobin A1c levels , for 21/2 years . - pubmed
Preoperatively , the acute insulin response and the rate of glucose disappearance ( Kg ) were 213 microU / mL and 2.14 % ( respectively ) after intravenous administration of 20 g of glucose . - pubmed
Five years posttransplantation he remains insulin - dependent with a fasting serum C - peptide level of 0.20 ng / mL , which increased to 0.35 ng / mL in response to intravenous arginine , indicating sustained islet function . - pubmed
These changes are dependent on calcium mobilization and calcineurin activation , since they are also elicited by ionomycin and are blocked by the immunosuppressive drug cyclosporin A. - pubmed
Deactivation of NFAT1 is facilitated by phorbol 12 - myristate 13 - acetate and inhibitors of capacitative calcium entry and most likely reflects the slow return of intracellular free calcium concentrations towards resting levels . - pubmed
Our results suggest that calcineurin - dependent signalling pathways mediate the early activation of NFAT1 , while phorbol 12 - myristate 13 - acetate - dependent feedback pathways contribute to the late deactivation . - pubmed
Pleural macrophages were isolated 72 h after intrapleural TCN or intrapleural carrageenan ( CAR ) , a substance that recruits pleural macrophages without producing pleural fibrosis . - pubmed
Macrophage cultured for 24 h produced a conditioned medium that was added to pleural mesothelial cell culture containing [ 3H ]- glucosamine and was compared to control cultures treated with RPMI culture media alone or with the addition of TCN or CAR . - pubmed
After 72 h , GAGs were isolated by pronase digestion , cetyl pyridinium precipitation , and MgCl2 and ethanol extraction . - pubmed
Isoproterenol regulates tumour necrosis factor , interleukin-10 , interleukin-6 and nitric oxide production and protects against the development of vascular hyporeactivity in endotoxaemia . - pubmed
Pro - inflammatory cytokines , such as tumour necrosis factor ( TNF ) and free radicals , such as nitric oxide ( NO ) , are mediators of endotoxaemia . - pubmed
Cyclic AMP can modulate endotoxin - induced cytokine and NO production . - pubmed
The hyporeactivity is due to expression of the inducible isoform of NO synthase ( iNOS ) and was restored by in vitro administration of NG - methyl - L - arginine ( L - NMA ) , an inhibitor of NO synthase . - pubmed
RESULTS : Serum sodium and potassium concentrations decreased during the race , as did serum total protein , albumin , and globulin concentrations and PCV . - pubmed
Endocytic uptake of lysophosphatidylcholine mediated by macrophage scavenger receptor plays a major role in oxidized low density lipoprotein - induced macrophage growth . - pubmed
The role of the methionine loading test ( MLT ) is , however , controversial . - pubmed
Hyperhomocysteinemia was found in 141 ( 33 % ) of the 429 subjects : 15 % were diagnosed by fasting homocysteine concentration and 18 % by MLT . - pubmed
Folic acid was lower in the group with an elevated fasting homocysteine concentration than in those with only an abnormal MLT result ( 11 versus 15 nmol / L , p = 0.002 ) . - pubmed
In order to characterize adenosine effects on quiescent peripheral blood mononuclear cells ( PBMC ) , we have evaluated the ability of the previously characterized adenosine receptor agonist 2 - chloro - adenosine ( 2CA ; Ceruti , Barbieri et al . - pubmed
The effect of 2CA was partially prevented by addition of dipyridamole ( DP ) , a nucleoside transport inhibitor , hence some of the apoptotic effect of this nucleoside is , at least in part , due to intracellular action . - pubmed
Alternatively , DP did not affect 2CdA - induced apoptosis , suggesting that 2CdA may enter cells via a DP - insensitive transporter . - pubmed
Data suggest that , for 2CA , apoptosis is partially sustained by activation of the A2A receptor subtype , whereas no role is exerted by P1 receptors in 2CdA - dependent apoptosis . - pubmed
Moreover , in these cells , apoptosis could also be triggered through intense activation of the A3 receptor via selective agonists such as 2 - chloro - N6 -( 3 - iodobenzyl ) adenosine-5 '- N - methyluronamide ( Cl - IB - MECA ) , but this mechanism plays no role in either 2CA- or 2CdA - induced apoptosis . - pubmed
Rab proteins are geranylgeranylated on one or two C - terminal cysteines by Rab geranylgeranyl transferase ( RabGGTase ) . - pubmed
We measured an equilibrium dissociation constant of 0.2 microM for the formation of a 1:1 REP - Rab complex and showed that this interaction relies mostly on ionic bonds and does not involve the two C - terminal cysteine residues . - pubmed
Third , we show that free REP inhibits the geranylgeranylation reaction , suggesting that the complex is recognized by RabGGTase primarily via a REP - binding site . - pubmed
MPP + decreases the nascent heavy strands per mtDNA and increases the transcript of the ND6 gene , encoded on light strand , per mtDNA . - pubmed
Four nodules enhanced by Gd DTPA were seen at C1 - C4 . - pubmed
In these cells the protein was effectively expressed and ICA69 carrying C - terminal histidine - hexapeptide could be efficiently purified using immobilized metal chelate affinity chromatography . - pubmed
beta - catenin is known as one of the adhesive molecules associated not only with cell adhesion and cell polarity , but also with carcinogenesis . - pubmed
The Secretor ( Se ) gene and Lewis ( Le ) gene are involved in type I Le antigen synthesis . - pubmed
The present study was performed to investigate the possibility that Se and Le gene polymorphisms alter the risk of H. - pubmed
Two hundred thirty - nine participants were genotyped for Se and Le and tested for the presence of anti - H. pylori IgG antibodies . - pubmed
Using the normal gastric mucosa from 60 gastric cancer patients , we assessed immunohistochemically whether type I Le antigen expression depended on the Se and Le genotypes . - pubmed
pylori infection rate was positively associated with the number of Se alleles ( se / se group , 45.1 % ; Se / se group , 64.6 % ; and Se / Se group , 73.3 % ) and negatively associated with the number of Le alleles ( le / le group , 76.4 % ; Le / le group , 68.3 % ; and Le / Le group , 55.6 % ) . - pubmed
Immunohistochemical analysis supported the finding that Se and Le genotypes affected the expression of H. - pubmed
We conclude that Se and Le genotypes affect susceptibility to H. - pubmed
There was up to a 37 - fold increase in expression of DR5 following treatment with ADR or VP-16 only in cells with wt p53 . - pubmed
A direct correlation was observed between enhanced DR5 expression and sensitivity to ADR and VP-16 . - pubmed
There was no induction of DR5 following treatment with VCR , MTX or DEX . - pubmed
There was up to a 51 - fold increase in the median level of expression of Fas following treatment with ADR and VP-16 , and unlike DR5 this occurred in cells with either wild - type or mutant p53 . - pubmed
Conversely , there was only a two - fold increase in expression of Fas after exposure to VCR , MTX and DEX . - pubmed
These findings suggest that DR5 mediates sensitivity to ADR and VP-16 in a p53 - dependent manner , whereas , Fas appears to mediate sensitivity to these two drugs independent of p53 status . - pubmed
DR5 and Fas do not appear to play a major role as determinants of chemosensitivity to VCR , MTX and DEX . - pubmed
Alternatively , effector pathways emanating from a specific Rho GTPase may be under- or overactivated . - pubmed
In this review , we address the role of the Rho - type GTPases as a subfamily of the Ras - superfamily of small GTP - binding proteins in the development of various disease phenotypes . - pubmed
Circulating activated platelets ( P - selectin / CD63 - positive platelets ) increased during HD sessions with cellulose diacetate and polysulfone . - pubmed
Aedes salivary homogenate hydrolyses inosine and guanosine to hypoxanthine and xanthine plus the ribose moiety , but does not hydrolyse the pyrimidines uridine and cytidine , thus characterizing the presence of a purine nucleosidase activity . - pubmed
Thus , MsGC - II appears to be a neural - specific receptor guanylyl cyclase whose activity may be regulated either directly or indirectly by calcium . - pubmed
RESULTS : The size of the variable region of AMA-1 gene from FCC1 / HN was 506 bp and encoded 168 amino acids . - pubmed
The levels and relative ratios of PI3 K products , phosphatidylinositol ( 3,4 ) bisphosphate ( PI ( 3,4 ) P ( 2 ) ) and phosphatidylinositol ( 3,4,5 ) trisphosphate ( PIP ( 3 ) ) , are regulated by inositol phosphatases such as Pten and SHIP . - pubmed
Here we established a new real - time assay to assess an imbalance of DNA SSB repair by indirectly measuring PARP-1 activation through the depletion of intracellular NAD ( P ) H. - pubmed
Using this assay , we demonstrated that the dose- and time - dependent depletion of NAD ( P ) H in XRCC1 - deficient CHO cells exposed to methyl methanesulfonate . - pubmed
Furthermore , methyl methanesulfonate reduced NAD ( P ) H in PARP-1+/+ cells , whereas PARP-1-/- cells were more resistant to the decrease in NAD ( P ) H. - pubmed
These results indicate that the analysis of intracellular NAD ( P ) H level using water - soluble tetrazolium salt can assess an imbalance of SSB repair in living cells in real time . - pubmed
When the EG - VEGF gene - overexpressing colorectal cancer cell line that had been treated with phosphorothioate antisense EG - VEGF oligonucleotides was injected s.c . - pubmed
Phase II trials using docetaxel monotherapy have demonstrated responses rates concerning PSA of 45 to 58 % and objective response of 33 % and 40 % . - pubmed
A randomized phase II trial revealed the superiority of this combination compared to mitoxantrone in terms of response rate and clinical benefit . - pubmed
Cytochrome P450 2E1 ( CYP2E1 ) enzyme catalyzes the metabolism of many procarcinogens , such as N - nitrosamines and related compounds . - pubmed
IL-4 and IL-13 potentiated CHX - induced apoptosis in basal cells but not in columnar cells , possibly due to low levels of the IL-4 receptor . - pubmed
The initial rate of DNA loss was estimated to be 0.05 bp per chromosome per generation for a WT bacterium and approximately 50 - fold higher for a mutS mutant defective in methyl - directed DNA mismatch repair . - pubmed
The testes were collected in November ( n = 15 ; testis recrudescence ) , February ( n = 15 ; before breeding season ) , March ( n = 14 ; breeding season ) , and May ( n = 11 ; testis involution ) and the following parameters of testicular activity were quantified : testicular mass , number of testicular spermatozoa , percentages of haploid , diploid , and tetraploid ( G2 / M - phase ) cells and content of testosterone and EGF . - pubmed
Testis mass , spermatogenic activity , and the production of both testosterone and EGF were maximal in March , but were not significantly different from the levels in February . - pubmed
Testosterone and EGF levels were correlated to each other ( r = 0.78 ; P & lt ; 0.001 ) and had significant positive correlations to testis mass , number of sperm and proportion of haploid cells ; and negative correlations to percentages of mitotic cells . - pubmed
We inferred that intensified seasonal spermatogenesis was stimulated by testosterone and by autocrine / paracrine effects of EGF . - pubmed
RESULTS : In the challenge model , Ciloxan , Ocuflox , and endotoxin all produced higher median DLK scores than the BSS control ( P = .02 ) . - pubmed
Ciloxan produced significant DLK in more eyes and had higher median scores ( 70 % , 1.0 ) than Ocuflox ( 40 % , 0.5 ) or endotoxin ( 45 % , 0.5 ) ( P = .05 ) . - pubmed
In the endotoxin - induced model , Ciloxan produced significantly higher DLK scores than Ocuflox or BSS ( P = .05 ) . - pubmed
Ocuflox was associated with less DLK than Ciloxan . - pubmed
The activity of the cardiac Na (+)- Ca ( 2 + ) exchanger ( NCX1.1 ) is allosterically regulated by Ca ( 2 + ) , which binds to two acidic regions in the cytosolically disposed central hydrophilic domain of the NCX protein . - pubmed
However , when cytosolic [ Na (+ ) ] was increased to 20 mm or more ( using ouabain - induced inhibition of the Na (+) , K (+)- ATPase or the ionophore gramicidin ) , cells expressing the D447V mutant rapidly accumulated Ca ( 2 + ) or Ba ( 2 + ) when the reverse ( Ca ( 2 + ) influx ) mode of NCX activity was initiated , although initial cytosolic [ Ca ( 2 + ) ] was & lt ; 100 nm . - pubmed
Importantly , the time course of Ca ( 2 + ) uptake did not display the lag phase that reflects allosteric Ca ( 2 + ) activation of NCX activity in the wild - type NCX1.1 ; indeed , at elevated [ Na (+ ) ] , the D447V mutant behaved similarly to the constitutively active deletion mutant Delta(241 - 680 ) , which lacks the regulatory Ca ( 2 + ) binding sites . - pubmed
In cells expressing wild - type NCX1.1 , increasing concentrations of cytosolic Na (+ ) led to a progressive shortening of the lag phase for Ca ( 2 + ) uptake . - pubmed
The results showed that PC was completely separated from phosphatidylethanolamine ( PE ) by the elution with 95 % alcohol , and its purity and yield reached more than 90 % and 80 % with a elution volume of 225 mL , and 87.6 % and 87.3 % with a elution volume of 425 mL respectively . - pubmed
Arbuscular mycorrhiza ( AM ) was greatly reduced by CdCl ( 2 ) amendment , and fluorescent pseudomonad inoculation significantly increased AM colonisation in the contaminated soil . - pubmed
Here we report the crystal structure at 3.2 - A resolution of an atToc33 mutant , atToc33(R130A ) , in which Arg(130 ) was mutated to alanine . - pubmed
Molecular modeling using the structures of psToc34 and atToc33(R130A ) suggests that , in an architectural dimer of atToc33 , Arg(130 ) from one monomer interacts with the beta - phosphate of GDP and several other amino acids of the other monomer . - pubmed
These results indicate that Arg(130 ) is critical for dimer formation , which is itself important for GTPase activity . - pubmed
Neither Na(+ ) nor Cl (- ) appears to be a transported substrate for Bor1p . - pubmed
Uphill efflux of boron mediated by Bor1p was demonstrated directly by loading cells with boron and resuspending in a low - boron medium . - pubmed
Cells with intact BOR1 , but not the deletant strain , transport boron outward until the intracellular concentration is sevenfold lower than that in the medium . - pubmed
Boron efflux through Bor1p is a saturable function of intracellular boron ( apparent K(m ) approximately 1 - 2 mM ) . - pubmed
Addition of 30 mM HCO ( 3 )(- ) does not affect boron extrusion by Bor1p , indicating that HCO ( 3 )(- ) does not participate in Bor1p function . - pubmed
Functional Bor1p is present in cells grown in medium with no added boron , and overnight growth in 10 mM H ( 3 ) BO ( 3 ) causes only a small increase in the levels of functional Bor1p and in BOR1 mRNA . - pubmed
Tight glucose control by intensive insulin treatment markedly improves clinical outcome . - pubmed
OBJECTIVE AND DESIGN : This is a randomized controlled trial comparing blood glucose control by a laptop - based model predictive control algorithm with a variable sampling rate [ enhanced model predictive control ( eMPC ) ; version 1.04.03 ] against a routine glucose management protocol ( RMP ) during the peri- and postoperative periods . - pubmed
RESULTS : Mean blood glucose was 6.2 + /- 1.1 mmol / liter in the eMPC vs. 7.2 + /- 1.1 mmol / liter in the RMP group ( P & lt ; 0.05 ) ; percentage of time in the target range was 60.4 + /- 22.8 % for the eMPC vs. 27.5 + /- 16.2 % for the RMP group ( P & lt ; 0.05 ) . - pubmed
Mean insulin infusion rate was 4.7 + /- 3.3 IU / h in the eMPC vs. 2.6 + /- 1.7 IU / h in the RMP group ( P & lt ; 0.05 ) . - pubmed
In a survey of 637 children , 603 ( 95 % ) were positive for antibody to hepatitis surface antigen ( anti - HBs ) without concomintant antibody to hepatitis B core antigen ( anti - HBc ) or hepatitis B surface antigen ( HBsAg ) . - pubmed
The regulatory effect of substance P on respiration is mediated via neurokinin ( NK ) receptors . - pubmed
Calcium - dependent gating of the large - conductance Ca ( 2 +)-activated K (+ ) ( BK(Ca ) ) channel is conferred by the large cytosolic carboxyl terminus containing two domains of the regulator of K (+ ) conductance ( RCK ) and the high - affinity Ca ( 2 +)-binding site ( the Ca ( 2 +)-bowl ) . - pubmed
The mood stabilizers lithium and valproate selectively activate the promoter IV of brain - derived neurotrophic factor in neurons . - pubmed
Surprisingly , lithium- or VPA - responsive element(s ) in promoter IV resides in a region upstream from the calcium - responsive elements ( CaREs ) responsible for depolarization - induced BDNF induction . - pubmed
Moreover , activation of BDNF promoter IV by lithium or VPA occurred in cortical neurons depolarized with KCl , and deletion of these three CaREs did not abolish lithium- or VPA - induced activation . - pubmed
Lithium and VPA are direct inhibitors of glycogen synthase kinase-3 ( GSK-3 ) and histone deacetylase ( HDAC ) , respectively . - pubmed
We showed that lithium - induced activation of promoter IV was mimicked by pharmacological inhibition of GSK-3 or short interfering RNA ( siRNA)-mediated gene silencing of GSK-3alpha or GSK-3beta isoforms . - pubmed
Furthermore , treatment with other HDAC inhibitors , sodium butyrate and trichostatin A , or transfection with an HDAC1 - specific siRNA also activated BDNF promoter IV . - pubmed
Our study demonstrates for the first time that GSK-3 and HDAC are respective initial targets for lithium and VPA to activate BDNF promoter IV , and that this BDNF induction involves a novel responsive region in promoter IV of the BDNF gene . - pubmed
The 15 kDa avidin monomer was fused to the carboxyl terminus of the heavy chain of the HIRMAb . - pubmed
Concentrations of the triamine spermidine and the tetramine spermine , as well as ornithine decarboxylase and S - adenosyl - methionine decarboxylase activities , decreased during sclerotia maturation . - pubmed
With a digoxigenin - labeled KS gene fragment used as a probe , a partial polyketide synthase ( PKS ) gene cluster was isolated and identified by hybridization screening of a marine sediment sample metagenome fosmid library constructed for this study . - pubmed
As for intermolecular interactions , we find a very significant difference in the evolving solvation pattern of the nucleophilic oxygen in water and in the enzyme . - pubmed
Cyfluthrin ( CAS no . - pubmed
Mitotic index ( MI ) , proliferation index ( PI ) and nuclear division index ( NDI ) decreased significantly ( P<0.05 ) due to the potential cytotoxicity of cyfluthrin , especially after the 48 - h treatment period . - pubmed
AIM : To evaluate the chemical activity of Portland cement and two other cement types with similar chemical composition to mineral trioxide aggregate with the aim of developing these cements for further applications in dentistry . - pubmed
Elevated serum triglyceride for men and low serum high density lipoprotein in women were the strongest predictors that effectively indicated the presence of metabolic syndrome in non - obese individuals . - pubmed
Release of nerve growth factor from HEMA hydrogel - coated substrates and its effect on the differentiation of neural cells . - pubmed
In this study , three different types of poly ( 2 - hydroxyethyl methacrylate ) ( pHEMA ) hydrogels were developed and assessed for storage capacity and release rates of the neurotrophin , nerve growth factor ( NGF ) . - pubmed
When targeted media concentrations were the same , responses to bath - applied NGF and NGF released from pHEMA hydrogels were not significantly different . - pubmed
When NGF was released from lysine - conjugated pHEMA hydrogels , a significant increase in process growth was observed . - pubmed
Our studies demonstrate that pHEMA coatings can be used on neural devices consistent with the needs for local neurotrophin delivery in the brain . - pubmed
Inhibition and binding kinetics of the hepatitis C virus NS3 protease inhibitor ITMN-191 reveals tight binding and slow dissociative behavior . - pubmed
Here , ITMN-191 is shown to be a potent inhibitor of NS3 with a two - step binding mechanism . - pubmed
From progress curve analysis , the rate constant for dissociation of ITMN-191 from the EI * complex is 3.8 x 10(-5 ) s(-1 ) with a calculated complex half - life of approximately 5 h and a true biochemical potency ( K(i ) * ) of approximately 62 pM. - pubmed
Abrogation of the tight binding and slow dissociative properties of ITMN-191 is observed with proteases that carry the R155 K or D168A substitution , each of which is likely in drug resistant mutants . - pubmed
Naltrexone has higher affinity for mu receptors than other opioid receptor subtypes . - pubmed
Thus , the effect of naltrexone in treating SIB appears to be due to ( mu - receptor interactions , with the loss of effect at higher doses due to supervening kappa receptor binding . - pubmed
Speculatively , one may ask if dosage reduction would convert naltrexone nonresponders to responders by lowering kappa receptor - mediated effects . - pubmed
In this study , in order to measure the noise - enhancing factor ( NEF ) , a nonlinear stochastic model is introduced for the Hodgkin - Huxley ( HH ) neuron system with synaptic noise input stimulation and channel noises in the sodium and potassium channels . - pubmed
Apoptosis was assessed using terminal digoxigenin - labeled dUTP nick - end labeling ( TUNEL assay ) and cell proliferation using MIB-1 monoclonal antibody by the alkaline phosphatase method . - pubmed
Our data suggest that TIMP-3 interacts with heparan sulfate and heparan sulfate proteoglycans and to a lesser extent with heparin and chondroitin sulfate . - pubmed
Aldose reductase ( AR ) is a multifunctional enzyme that reduces aldehydes . - pubmed
Under diabetic conditions AR converts glucose into sorbitol , which is then converted to fructose . - pubmed
In contrast , a recent report suggests that genetic ablation of AR increased atherosclerosis and increased hydroxynonenal in arteries . - pubmed
It was hypothesized that AR knockout prevented reduction of toxic aldehydes . - pubmed
The effect of ethanol and heat on the functional hydrophobicity of casein micelles . - pubmed
The changes to the hydrophobicity of milk proteins during that process were evaluated by using the fluorescent hydrophobic probe 1 - anilinonaphthalene-8 - sulfonic acid ( ANS ) . - pubmed
This resulted in a reduction in the functional hydrophobicity of the milk proteins as ethanol rendered the hydrophobic sites unavailable for interaction . - pubmed
The food - associated fungal neurotoxin ochratoxin A inhibits the absorption of glutamate by astrocytes through a decrease in cell surface expression of the excitatory amino - acid transporters GLAST and GLT-1 . - pubmed
Although OTA inhibits glutamine synthetase activity , this effect was not involved in OTA - mediated alteration of glutamate absorption since decrease in enzyme activity only occurred at high cytotoxic concentrations of toxin ( 100 microM ) . - pubmed
Similarly , alterations in the expression of the excitatory amino - acid transporters were not involved since OTA failed to modify total expression level of GLAST and GLT-1 . - pubmed
We found that inhibition of glutamate absorption by OTA was due to a decrease in the expression of GLAST and GLT-1 at the cell surface . - pubmed
Carboxyl ester lipase ( CEL ) is an enzyme that hydrolyzes a wide variety of lipid substrates , including ceramides , which are known to show inhibitory regulation of pituitary hormone secretion in experimental models . - pubmed
Considering that CEL hydrolyzes ceramides , inactivating their inhibitory function on pituitary hormone secretion , our findings suggest a possible role of CEL in the regulation of hormone secretion in both normal and adenomatous pituitary cells . - pubmed
Lipid - induced insulin resistance is associated with intracellular accumulation of inhibitory intermediates depending on the prevalent fatty acid ( FA ) species . - pubmed
In cultured myotubes , ceramide and phosphatidic acid ( PA ) mediate the effects of the saturated FA palmitate and the unsaturated FA linoleate , respectively . - pubmed
Saline , LSF ( 25 mg / kg x d ) , or MYR ( 0.3 mg / kg x d ) were administered by mini - pumps in the final 4 wk . - pubmed
Muscle ceramide and PA were analyzed by mass spectrometry . - pubmed
The discrepancy between ceramide and glucose tolerance suggests these improvements may not be related directly to changes in muscle ceramide and may involve other insulin - responsive tissues . - pubmed
Changes in the expression of LASS1 were , however , inversely correlated with alterations in glucose tolerance . - pubmed
To date , the large , phase III randomized controlled breast cancer prevention trials have tested and are testing only hormonal drugs designed to antagonize the carcinogenic effect of endogenous estrogen ; these agents are either selective estrogen receptor modulators ( SERMs ) or aromatase inhibitors ( AIs ) . - pubmed
Next we demonstrate how this brain clock controls plasma glucose concentrations , via projections to sympathetic and parasympathetic pre - autonomic neurons within the hypothalamus . - pubmed
In agreement with the fact that brain aromatase activity is correlated with sex steroid levels , the high expression of cyp19a1b is due to an autoregulatory loop through which estrogens and aromatizable androgens upregulate aromatase expression . - pubmed
Here , we identify a previously uncharacterized phosphorylation site on Ect2 , threonine 328 , that serves to regulate the oncogenic activity of Ect2 in NSCLC cells . - pubmed
PKCiota directly phosphorylates Ect2 at Thr-328 in vitro , and RNAi - mediated knockdown of either PKCiota or Par6 leads to a decrease in phospho - Thr-328 Ect2 , indicating that PKCiota regulates Thr-328 Ect2 phosphorylation in NSCLC cells . - pubmed
Rapamycin induced apoptosis as well as the expression of p21 ( waf1 ) mRNA and protein , independent of p53 . - pubmed
Rapamycin treatment also resulted in : a decrease in cdk2 kinase activity ; an increase in hypophosphorylated retinoblastoma protein ( pRb ) ; a dephosphorylation of p70 S6 kinase ; and , growth - arrest in G(1)-phase of cell cycle . - pubmed
These data suggest that rapamycin - induced growth arrest and apoptosis occur through the p53 - independent induction of p21 ( waf1 ) . - pubmed
Aspirin is an organic anion and could be a substrate for MRP4 . - pubmed
RESULTS : Inhibition of MRP4 - mediated transport by dipyridamole or Mk-571 increases aspirin entrapment and its in vitro effect on COX-1 activity ( 142.7 + - 34.6 pg/10(8 ) cells vs. 343.7 + - 169.3 pg/108 cells TxB2 - production ) . - pubmed
Platelets derived from megakaryocytes transfected with MRP4 small interfering ribonucleic acid have a higher aspirin entrapment and drug COX-1 activity . - pubmed
CONCLUSIONS : Aspirin is a substrate for MRP4 and can be extruded from platelet through its transportation . - pubmed
ATP - stimulated leukemic cells , but not normal CD34 + cells , undergo down - regulation of genes involved in cell proliferation and migration , whereas cell - cycle inhibitors are up - regulated . - pubmed
P2R - expression analysis and pharmacologic profiling suggested that the inhibition of proliferation by ATP was mediated by the down - regulation of the P2X7R , which is up - regulated on untreated blasts , whereas the inhibition of chemotaxis was mainly mediated via P2Y2R and P2Y4R subtypes . - pubmed
coli strains that were improved by an elongation cycle of fatty acid synthesis ( FAS ) . - pubmed
The beta - oxoacyl - ACP synthase enzyme catalyzed the addition of acyl - ACP to malonyl - ACP to generate beta - oxoacyl - ACP . - pubmed
The enzyme encoded by the fabG gene converted beta - oxoacyl - ACP to beta - hydroxyacyl - ACP , the fabZ catalyzed the dehydration of beta-3 - hydroxyacyl - ACP to trans-2 - acyl - ACP , and the fabI gene converted trans-2 - acyl - ACP to acyl - ACP for long - chain fatty acids . - pubmed
The wild - type PV ( WT ) as well as three mutant expression constructs were engineered : lysine 8 to asparagine ( K8N ) , lysine 26 to asparagine ( K26N ) and a double mutant ( DM ) . - pubmed
Calcium equilibrium dissociation constants ( K(d ) ) versus temperature curves for all mutants were right - shifted , as predicted , relative to that of WT PV . - pubmed
Calculated rates of Ca ( 2 + ) binding ( k(on ) ) for the K8N and K26N mutants were similar to values for the WT PV at all temperatures . - pubmed
In contrast , the calculated k(on ) values for the DM PV were faster , providing mechanistic insights into the nature of potentially adaptive changes in Ca ( 2 + ) binding in this PV . - pubmed
LC - HR / MS IN CLINICAL TOXICOLOGY : LC - HR / MS has been used to detect designer drugs , doping agents , ( neurosteroids ) and adulterants such as levamisole , a veterinary antihelmitic found in street cocaine , and pharmaceuticals in herbal medications marketed to contain only natural ingredients . - pubmed
The identification of novel designer amines , stimulants found in " bath salts " , and synthetic cannabinoids are well suited to LC - HR / MS . - pubmed
MetS was defined as the presence of three or more of the following : blood pressure>=130/90 mm Hg ; triglycerides>=150 mg / dL ; high - density lipoprotein<=50 mg / dL for women and & lt;=40 mg / dL for men ; body mass index>=30 kg / m2 ; or fasting blood glucose>=110 mg / dL. - pubmed
The distribution of MetS factors among all patients was hypertension in 98 % , diabetes in 58 % , elevated triclyceride in 39 % , decreased high - density lipoprotein in 60 % , elevated body mass index in 36 % , and 39 % were currently receiving hemodialysis . - pubmed
COMT , for example , encodes catechol - o - methyl transferase the enzyme primarily responsible for catabolizing dopamine in the prefrontal cortex . - pubmed
Striatal dopamine function , linked with cognitive tasks as well as habit learning , is influenced by the Taq - Ia polymorphism of the DRD2 / ANKK1 gene complex ; this gene influences the density of dopamine receptors in the striatum . - pubmed
Participants were genotyped for COMT ( Val(158)Met ) and DRD2 / ANKK1 - Taq - Ia ( A1 + , A1- ) polymorphisms . - pubmed
After CD3 + cell depletion , lung homogenates were re - stimulated 24 h with LPS ( 100 ng / ml ) , PG ( 10 mug / ml ) , IL-23 ( 100 ng / ml ) or vehicle . - pubmed
Therefore , the objective of this experiment was to evaluate the interactions between forage particle size ( FPS ) and ruminally fermentable carbohydrates ( RFC ) for dry matter intake ( DMI ) , ration sorting , ruminal fermentation , chewing activity , and milk yield and components . - pubmed
Increasing RFC was found to increase milk yield , milk protein content and yield , and lactose content and yield but decrease milk fat content . - pubmed
Progression - free survival was significantly better with docetaxel than with erlotinib : median progression - free survival was 2 9 months ( 95 % CI 2 4 - 3 8) with docetaxel versus 2 4 months ( 2 1 - 2 6 ) with erlotinib ( adjusted HR 0 71 , 95 % CI 0 53 - 0 95 ; p=0 02 ) . - pubmed
Transmission electron microscopy ( TEM ) and powder X - ray diffraction ( XRD ) evidenced the formation of pHA NCs and of their crystalline nature . - pubmed
The formation of Au nanoshell -- surrounding pHA NC -- was confirmed from its surface plasmon resonance ( SPR ) band in the UV / Vis spectrum and by TEM measurements . - pubmed
The dissolution of Au shell was marked by a gradual diminishing of its SPR band , while the release of pHA molecules in the solution was confirmed from TEM and FTIR studies . - pubmed
Synthetic retinoid Am80 up - regulates apelin expression by promoting interaction of RARalpha with KLF5 and Sp1 in vascular smooth muscle cells . - pubmed
Previous studies have demonstrated that both retinoids and apelin possess potent cardiovascular properties and that retinoids can mediate the expression of many genes in the cardiovascular system . - pubmed
In the present study , we investigated the molecular mechanism of apelin expression regulation by the synthetic retinoid Am80 in VSMCs . - pubmed
The results showed that Am80 markedly up - regulated apelin mRNA and protein levels in VSMCs . - pubmed
Furthermore , KLF5 ( Kruppel - like factor 5 ) and Sp1 ( stimulating protein-1 ) co - operatively mediated Am80 - induced apelin expression through their direct binding to the TCE ( transforming growth factor - beta control element ) on the apelin promoter . - pubmed
Interestingly , upon Am80 stimulation , the RARalpha ( retinoic acid receptor alpha ) was recruited to the apelin promoter by interacting with KLF5 and Sp1 prebound to the TCE site of the apelin promoter to form a transcriptional activation complex , subsequently leading to the up - regulation of apelin expression in VSMCs . - pubmed
Thus the results of the present study describe a novel mechanism of apelin regulation by Am80 and further expand the network of RARalpha in the retinoid pathway . - pubmed
Regarding bezafibrate - associated rhabdomyolysis and cibenzoline - associated hypoglycemia , these ADR were not detected in cases involving monotherapy . - pubmed
A tryptophan metabolite , kynurenine , promotes mast cell activation through aryl hydrocarbon receptor . - pubmed
BACKGROUND : Tryptophan metabolites have been suggested to play a role in immune modulation , wherein those have recently been shown to be endogenous ligands of aryl hydrocarbon receptor ( AhR ; a unique cellular chemical sensor ) . - pubmed
However , the involvement of tryptophan metabolites and AhR in modulating mast cell function remains to be fully defined . - pubmed
RESULTS : Kynurenine , but not KA and QA , enhanced IgE - mediated responses , including degranulation , LTC4 release , and IL-13 production in BMMCs through the activation of PLCgamma1 , Akt , MAPK p38 , and the increase of intracellular calcium . - pubmed
These enhancing effects of KYN were not observed in AhR - deficient BMMCs and could be inhibited by CH223191 in BMMCs . - pubmed
CONCLUSION : The AhR - KYN axis is potentially important in modulating mast cell responses and represents an example of AhR 's critical involvement in the regulation of allergic responses . - pubmed
In vivo , PDE4B deletion could attenuate the lung water content , histological signs of pulmonary injury and elevate the ratio of partial pressure of arterial O2 to fraction of inspired O2 ( PaO2 / FIO2 ratio ) . - pubmed
Treatment with a TGFbeta receptor inhibitor , galunisertib , reduced the number of arrested cells in brain to 808 + - 82 cells / brain . - pubmed
Furthermore , we observed a reduction in the percentage of extravasated cells ( from 63 % to 30 % ) compared with cells remaining intralumenal when TGFbeta is knocked down or inhibited with galunisertib ( 40 % ) . - pubmed
Additionally , K (+ ) channel blockers , such as tetraethylammonium ( TEA ) , apamin ( APA ) , and glibenclamide ( Glib ) , had no effect . - pubmed
However , N ( G )- nitro - L - arginine methyl ester ( L - NAME ) and 1H -( 1,2,4 ) oxadiazolo ( 4,3 - A ) quinoxalin-1 - one ( ODQ ) , an inhibitor of soluble guanylate cyclase ( sGC ) , did inhibit histamine - induced tonic relaxation . - pubmed
Ranitidine , the H2 receptor blocker , inhibited histamine - induced tonic relaxation . - pubmed
miRNAs are small noncoding RNAs of approximately 22 nucleotides length , which have emerged as important regulators of genes at post - transcriptional levels in a range of organisms . - pubmed
Although quinazolines are well known to inhibit EGFR , there is also a large panel of other therapeutic protein targets . - pubmed
EXPERT OPINION : Since 2011 , a lot of quinazoline compounds have shown EGFR inhibition . - pubmed
Therefore , several patents have been approved for the use of quinazoline compounds as inhibitors of other kinases , histone deacetylase , Nox and some metabolic pathways . - pubmed
Total vanadium was determined by inductively coupled plasma - mass spectrometry ( ICP - MS ) and speciation by ion chromatography - ICP - MS ( IC - ICP - MS ) . - pubmed
Intravenous ( IV ) iron treatment was determined by HCPCS codes , prescription oral iron by NDC numbers , and blood transfusions by CPT and ICD-9 - CM codes . - pubmed
Although most bariatric surgery patients who develop IDA are tested for iron , most are not treated with IV iron or oral iron and do not receive blood transfusions . - pubmed
Inhibition of PKC - delta activation with rottlerin significantly increased the survival rates and decreased serum ALT / AST levels at 6 , 12 and 24 h compared with the control group ( P & lt ; 0.001 ) . - pubmed
Rottlerin treatment also significantly decreased serum levels of HMGB1 at 6 , 12 , and 24 h , TNF - alpha , IL-6 and IL-1 beta at 12 h compared with the control group ( P & lt ; 0.01 ) . - pubmed
[ Overexpressing 3 - ketosteroid - Delta1 - dehydrogenase for degrading phytosterols into androst-1,4 - diene-3,17 - dione ] . - pubmed
Depolarization of mitochondria in neurons promotes activation of nitric oxide synthase and generation of nitric oxide . - pubmed
We examined for the first time the effects of mitochondrial depolarization on mitochondrial function , intracellular calcium , neuronal nitric oxide synthase ( nNOS ) activation , and nitric oxide ( NO ) production in cultured neurons and perivascular nerves . - pubmed
In addition , diazoxide and BMS not only increased the nNOS phosphorylation at positive regulatory serine 1417 but also decreased nNOS phosphorylation at negative regulatory serine 847 . - pubmed
Furthermore , diazoxide and BMS increased NO production in cultured neurons measured with both fluorescence microscopy and electron spin resonance spectroscopy , which was sensitive to inhibition by the selective nNOS inhibitor 7 - nitroindazole ( 7 - NI ) . - pubmed
Diazoxide also protected cultured neurons against oxygen - glucose deprivation , which was blocked by NOS inhibition and rescued by NO donors . - pubmed
Thus pharmacological depolarization of mitochondria promotes activation of nNOS leading to generation of NO in cultured neurons and endothelium - denuded arteries . - pubmed
The cardioprotective effect of ischemic postconditioning was eliminated after inhibition of protein kinase C with cheleritrin or after blockade of delta - isoform of protein kinase C with rottlerin . - pubmed
alternans essential oil by GC and GC - MS and evaluated its antimicrobial and antioxidant activity by disc diffusion , DPPH , ABTS and FRAP methods , respectively . - pubmed
Here we have considered the reaction mechanistic chemistry of epoxides and combined published experimental kinetic data ( rate constants k for reaction with a cysteine - based peptide ) together with calculated hydrophobicity data ( logP ) to derive a QMM correlating potency in the local lymph node assay ( LLNA ) , expressed as EC3 , with a relative alkylation index ( RAI , calculated as logk + 0.4 logP ) . - pubmed
The epoxide QMM predicts the potency of a nonepoxide SN2 electrophile ( predicted EC3 , 0.48 % ; observed EC3 , 0.5 % ) , which suggests that it could form the basis for a more general H - polar SN2 QMM that could be a valuable tool in skin sensitization risk assessment for this quite extensive and structurally diverse reaction mechanistic domain . - pubmed
The putative molecular target of IDPP is glycoprotein E1 , as revealed by selection for viral drug resistance ( Gly-257 - Arg ) . - pubmed
CEA was performed at 30 d after craniotomy , comparing the following strategies : mechanical prophylaxis ( MP ) , low molecular weight heparin with MP ( MP+LMWH ) , and unfractionated heparin with MP ( MP+UFH ) to prevent symptomatic VTE . - pubmed
Single CpG site methylation controls estrogen receptor gene transcription and correlates with hormone therapy resistance . - pubmed
In addition , luciferase reporter assays showed reduced transcriptional activity from this methylated CpG site . - pubmed
This CpG region was also completely conserved among species , suggesting that it acts as a methylation - sensitive Ets-2 transcription factor binding site , as confirmed using chromatin immunoprecipitation assays . - pubmed
In estrogen receptor alpha - positive tumors , CpG4 methylation levels were inversely correlated with estrogen receptor alpha expression status , suggesting that single CpG site plays an important role in the regulation of estrogen receptor alpha transcription . - pubmed
jirovecii that could evolve towards PCP , raising the issue of cotrimoxazole prophylaxis for at - risk quantitative polymerase chain reaction ( qPCR)-positive immunocompromised patients . - pubmed
Here we show that metformin promotes global DNA methylation in non - cancerous , cancer - prone and metastatic cancer cells by decreasing S - adenosylhomocysteine ( SAH ) , a strong feedback inhibitor of S - adenosylmethionine ( SAM )- dependent DNA methyltransferases , while promoting the accumulation of SAM , the universal methyl donor for cellular methylation . - pubmed
Using metformin and a mitochondria / complex I ( mCI)-targeted analog of metformin ( norMitoMet ) in experimental pairs of wild - type and AMP - activated protein kinase ( AMPK)- , serine hydroxymethyltransferase 2 ( SHMT2)- and mCI - null cells , we provide evidence that metformin increases the SAM : SAH ratio - related methylation capacity by targeting the coupling between serine mitochondrial one - carbon flux and CI activity . - pubmed
By increasing the contribution of one - carbon units to the SAM from folate stores while decreasing SAH in response to AMPK - sensed energetic crisis , metformin can operate as a metabolo - epigenetic regulator capable of reprogramming one of the key conduits linking cellular metabolism to the DNA methylation machinery . - pubmed
CONCLUSION : Simultaneous inhibition of EGFR and IGF1R in cisplatin - resistant TNBC cell lines was synergistic regarding inhibition of proliferation and induction of apoptosis . - pubmed
This assay , together with molecular modeling studies allowed us to select compound zr17 - 2 for an in vivo experiment , which showed an interesting increase of CIRBP expression in several organs of experimental animals . - pubmed
Ferredoxin - dependent bilin reductases ( FDBRs ) are a class of enzymes reducing the heme metabolite biliverdin IXalpha ( BV ) to form open - chain tetrapyrroles used for light - perception and light - harvesting in photosynthetic organisms . - pubmed
Phylogenetically , PcyA is the closest relative catalysing the reduction of BV to phycocyanobilin . - pubmed
Biochemical data in combination with the PcyX crystal structure revealed that a conserved aspartate - histidine pair is critical for activity . - pubmed
Interestingly , the same residues are part of a catalytic Asp - His - Glu triad in PcyA , including an additional Glu . - pubmed
While this Glu residue is replaced by Asp in PcyX , it is not involved in catalysis . - pubmed
Substitution back to a Glu failed to convert PcyX to a PcyA. - pubmed
Multi - facet implications of PEGylated lysozyme stabilized - silver nanoclusters loaded recombinant PTEN cargo in cancer theranostics . - pubmed
Herein , we report successful assembly of therapeutically significant phosphatase protein PTEN and fluorescent lysozyme - stabilized silver nanoclusters to accomplish delivery and tracking of the protein . - pubmed
The integration of purified recombinant PTEN with silver nanoclusters was evaluated by fluorescence spectroscopy study . - pubmed
Prior to cellular application , the functional integrity of PTEN in the composite was determined in vitro , by enzymatic assay employing para - nitrophenylphosphate as substrate . - pubmed
Thus , PEG coated GST - PTEN loaded silver nanoclusters serves as a comprehensive system encompassing cellular imaging and protein delivery with potential biomedical implications . - pubmed
Eighteen organic contaminants were detected in groundwater in the PRD , where the most frequently detected organic contaminant was naphthalene , and its detection rate was 2.51 % . - pubmed
Anthracyclines , paclitaxel , carboplatin , and docetaxel were the CT agents producing the highest taste disturbance rates . - pubmed
In concordance with the metabolome data , metagenomics prediction analysis based on 16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with indole alkaloid biosynthesis , cytochrome P450 and intestinal homeostasis , which coincided with up - regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon . - pubmed
Community - dwelling people with dementia treated with warfarin had poorer anticoagulation control than those without dementia ( mean time in therapeutic range ( TTR ) % + -SD , 38 + -26 ( dementia ) , 61 + -27 ( no dementia ) , p & lt ; 0.0001 ) . - pubmed
Synthesis and insecticidal activity in vitro and vivo of novel benzenesulfonyl derivatives based on potent target subunit H of V - ATPase . - pubmed
Two lead compounds with benzenesulfonamide were found through virtual screening based on the 3D structure of the subunit H of V - ATPase in previous study . - pubmed
Finally , carnosine has been shown to exert a multimodal activity including inhibition of protein cross - linking and aggregation of amyloid - beta and related proteins , free radical generation , nitric oxide detoxification , and an anti - inflammatory activity . - pubmed
brassicae by whole - cell fatty acid ( WCFA ) measurements and real - time polymerase chain reaction ( PCR ) was evaluated . - pubmed
Design , synthesis and biological evaluation of amide - pyridine derivatives as novel dual - target ( SE , CYP51 ) antifungal inhibitors . - pubmed
Based on the analysis of the squalene cyclooxygenase ( SE ) and 14alpha - demethylase ( CYP51 ) inhibitors pharmacophore feature and the dual - target active sites , a series of compounds with amide - pyridine scaffolds have been designed and synthesized to treat the increasing incidence of drug - resistant fungal infections . - pubmed
Urinary total BPA ( free and conjugated ) concentration was quantified using the HPLC - MS / MS . - pubmed
Participants were further stratified into thyroid autoantibody positive group ( at least one positive ) and thyroid autoantibody negative group ( both negative ) according to the thyroglobulin antibody ( TGAb ) and thyroid peroxidase antibody ( TPOAb ) levels , and restricted cubic spline regression was also applied to determine the possible nonlinear relationship between urinary BPA and TNs . - pubmed
RESULTS : Compared with women in the first quartile , the odds of TNs was 72 % ( adjusted OR = 1.72 , 95 % CI : 1.25 to 2.35 ) higher for those in the second quartile , 54 % ( adjusted OR = 1.54 , 95 % CI : 1.12 to 2.12 ) higher for those in the third quartile , and 108 % ( adjusted OR = 2.08 , 95 % CI : 1.50 to 2.90 ) higher for those in the fourth quartile after adjusting for age , BMI , education , HDL - C , LDL - C , triglyceride , total cholesterol , urinary iodine , TGAb and TPOAb . - pubmed
All strains , including fluoroquinolone - resistant and/or extended - spectrum beta - lactamase - producing strains , were multi - drug resistant . - pubmed
Mechanistically , we found that mitochondrial CHCHD10 is required for ATP production , which facilitates AChR expression and promotes agrin - induced AChR clustering . - pubmed
Importantly , ATP could effectively rescue the reduction of AChR clusters in the CHCHD10 - ablated muscles . - pubmed
At 25 C , the increase of non - photochemical quenching ( NPQ ) values in cells exposed to 10.0 ppm Ni might indicate the onset of thermal dissipation process as a self - protection mechanism against Ni toxicity . - pubmed
Romiplostim is a peptibody with c - MPL agonist activity that stimulates endogenous thrombopoietin production and leads to promoting the proliferation and differentiation of megakaryocytes in the bone marrow . - pubmed
Herein , a rationally designed a Cu ( II )-based metal - organic gel ( Cu - MOG ) with a nanoporous 3D network structure prepared via a simple one - step mixing method was successfully employed for the removal of cationic dyes . - pubmed
The Cu - MOG exhibited high efficiency , with an adsorption capacity of up to 650.32 mg / g , and rapid adsorption efficiency , with the ability to adsorb 80 % of Neutral Red within 1 min . - pubmed
Remarkably , the Cu - MOG displayed size - selective adsorption , based on adsorption studies concerning dyes of different sizes as calculated by density functional theory . - pubmed
Additionally , the adsorption performance of the Cu - MOG still maintained removal efficiency of 100 % after three regeneration cycles . - pubmed
These results suggested that the Cu - MOG could be expected to be a promising and competitive candidate to conveniently process wastewater . - pubmed
Then , we measured the protein and mRNA expression of stathmin , p - stathmin ( Ser16 , Ser25 , Ser38 , and Ser63 ) , beta - tubulin , and MAP-1B in the hippocampus , amygdala , and mPFC in the 5 groups by immunohistochemistry , Western blotting , and qRT - PCR . - pubmed
The trend of the protein expression of p - stathmin ( Ser25 and Ser38 ) was opposite of that of stathmin ; it reached a peak on the 7th day , and then decreased in the hippocampus . - pubmed
The protein expression of p - stathmin ( Ser63 ) showed the same trend in the mPFC . - pubmed
Stathmin in the memory loop , especially in the hippocampus , regulates microtubule structure through its phosphorylation at Ser25 and Ser38 and thereby participates in the mediation of fear memory abnormalities in PTSD . - pubmed
Low - dose microcystin - LR antagonizes aflatoxin B1 induced hepatocarcinogenesis through decreasing cytochrome P450 1A2 expression and aflatoxin B1 - DNA adduct generation . - pubmed
These results suggest that when co - exposure with AFB1 , MC - LR might repress hepatocarcinogenicity of AFB1 , which might be associated with its repression on AFB1 induced CYP1A2 upregulation and activation . - pubmed
Efficient adsorption of certain trace organic chemicals ( TOrCs ) present in secondary treated municipal wastewater treatment plant ( WWTP ) effluents onto granular activated carbon ( GAC ) has already been demonstrated at lab- and full - scale . - pubmed
Due to high organic matter concentrations in WWTP effluents , GAC filters eventually develop a biofilm and turn into biological activated carbon filters ( BAC ) , where removal of organic compounds is governed by biodegradation as well as by adsorption . - pubmed
Capto L is an affinity resin based on Protein L , which binds to the variable region of kappa light chain without interfering with the antigen binding site . - pubmed
In this work , we demonstrated that Capto L provides a convenient means for separating 3/4 antibody from the bsAb product . - pubmed
The molecular hybridization approach was based on the combination of 1 - benzyl-4 - piperidine fragment from the anti - Alzheimer AChE inhibitor donepezil ( 1 ) and the cinnamoyl subunit from curcumin ( 2 ) , a natural product with remarkable antioxidant , neuroprotective and anti - inflammatory properties , using a N - acylhydrazone fragment as a spacer subunit . - pubmed
Similarly , compound 4c was capable to prevent the ROS formation , with indirect antioxidant activity increasing intracellular GSH levels and the ability to counteract the neurotoxicity induced by both OAbeta1 - 42 and 3 - NP . - pubmed
Results of a two - compartment kinetic model demonstrated that aromatic components of MBCs dominated slow - sorption mechanisms , whereas the embedded magnetite further accelerated fast - sorption due to H - bonding . - pubmed
The degradation of SMT occurred because of persistent free radicals ( PFRs ) on BCs or the inherent redox of iron minerals on MBCs . - pubmed
The results showed that YM preadministration to H2O2 exposition significatively increased cell adhesion after 3 days as well as proliferation and in situ ALP detection after 10 and 7 days respectively , when compared to H2O2 group . - pubmed
Besides , staining of OCN and BSP proteins was less intense and scattered in poor spread cells with cytoskeletal changes in H2O2 group when compared to control and YM H2O2 group . - pubmed
It was found that ( a ) HMP stimulation could be selectively inhibited under conditions where release of granule enzymes remained unchanged or was enhanced , for example , by reduced glucose concentration or by 2 - deoxyglucose . - pubmed
The samples were assayed for acid phosphatase content by a colorimetric method using sodium thymolphthalein monophosphate as a substrate and by 2 immunochemical assays developed at our laboratory ( counter immunoelectrophoresis and radioimmunoassay ) . - pubmed
Thus , the depression of KC antigen presentation , as well as the enhanced capacity of these cells to release inflammatory mediators ( TNF , IL-1 , IL-6 and prostanoids ) which may produce cell and organ dysfunction , could contribute to the host 's enhanced susceptibility to sepsis following hemorrhage . - pubmed
The statistical properties of the bursting potassium channel from fused Torpedo synaptosomes were studied by using patch - clamp recording and time series analysis . - pubmed
When the injection was given at 1030 h , ICI 154,129 ( 50 micrograms ) exerted no significant effects on either LH or PRL . - pubmed
As newer agents become available ( eg , beta - blockers with ancillary properties , calcium channel blockers with allegedly more selective actions on various vascular beds ) , comparative studies between these agents and ACE inhibitors old and new are awaited with interest . - pubmed
Neither CT - A subunit nor CT - B subunit alone caused [ 3H ] AA release . - pubmed
The effect of CT on AA metabolism is proposed as a possible mechanism leading to the synthesis of prostaglandin E and fluid secretion during cholera . - pubmed
Monastral blue ( MB ) was used as a vascular marker and was infused into the pulmonary artery catheter either for recirculation at time zero or as an end - experiment ( 60 min ) bolus . - pubmed
The reconstruction of two - dimensional spatial images with 0.5 - mm resolution using radiofrequency gradients generated by an NMR coil system rotated about the sample is demonstrated for 23Na . - pubmed
Binding of C3 on the surface of infected cells was measured in a biotin - streptavidin amplified ELISA , and complement - mediated lysis was measured in a 51Cr release assay . - pubmed
A proton transfer triggered by a ligand interacting with the receptor had been suggested as the initial step in the activation of a receptor for the neurotransmitter serotonin ( 5 - hydroxy - tryptamine ; 5 - HT ) . - pubmed
With regard to transepithelial Na transport , the data suggest that ADH stimulates transport by increasing the Na permeability of the apical membranes of granular cells specifically . - pubmed
Amiodarone is known to affect thyroid homeostasis by competitive inhibition of 5 '- monodeiodinase , which converts L - thyroxine ( T4 ) to triiodothyronine ( T3 ) and reverse T3 ( rT3 ) to 3,3 '- diodothyronine ( T2 ) , and also by the direct effects of its high iodine content ( 37 % by weight ) . - pubmed
These changes were considered to be compensatory adjustments by the pituitary - thyroid axis to competitive inhibition of 5 '- monodeiodinase by the amiodarone . - pubmed
Explants cultured within 2 h of expulsion following prostaglandin induced termination secreted 3.0 times more insulin after 20 days of culture than those cultured within 2 - 4 h and 8.1 times more than those cultured more than 4 h post - termination . - pubmed
Fresh tissue responded to a high concentration of glucose ( 19.3 mM ) with a small but significant increase in insulin secretion . - pubmed
The addition of 10 mM theophylline caused a major increase in insulin release . - pubmed
Cultured tissue did not respond to glucose alone but did not show increased insulin release following stimulation with glucose ( 22 mM ) together with theophylline ( 10 mM ) in static incubation . - pubmed
A pharmacokinetic interaction between clomipramine and morphine was excluded ; involvement of change in opiate and 5 HT2 receptors by chronic administration of clomipramine is discussed . - pubmed
Antibody studies and aminoacid analysis indicated that it was albumin . - pubmed
Study of electrolytic labeling of fibrinogen with 131 - iodine by Sephadex G-10 gel filtration . - pubmed
The predicted 403 - amino acid preprorenin consists of mature renin and a 66 - residue amino - terminal prepropeptide . - pubmed
The possibility that the spectrum of intermediate two , seen in the course of reaction of flavoenzyme phenol hydroxylases , may be attributable to iminol isomers of a flavin - derived 6 - arylamino-5 - oxo ( 3H,5H ) uracil . - pubmed
A commonly held view of the mechanism of flavin mixed - function oxidases is that enzyme - bound 4a - hydroperoxyflavin ( 4a - FIHOOH ) undergoes ring opening to provide a carbonyl oxide ( IV ) , which , after transferring an oxene equivalent to substrate , yields a 6 - arylamino-5 - oxo ( 3H,5H )- uracil ( I ) . - pubmed
Comparison of the spectra of III ( and II ) , taken in solvents of widely differing dielectric constants and in a strongly basic medium , with those of the intermediate(s ) observed to be formed in time between 4a - FlHOOH and 4a - FlHOH has shown that the enzyme - bound intermediate(s ) does not resemble spectrally I nor its iminol tautomers . - pubmed
For LiF TLD in all the media agreement with experiment is improved . - pubmed
Both spontaneous and GC - induced apoptosis were increased by the macromolecular synthesis inhibitors , cycloheximide ( CHX ) and puromycin . - pubmed
Thimerosal ( 100 - 500 microM ) induced a DTT - reversible block of partially rundown KATP channels , or channels that underwent complete rundown ; these channels were reactivated with trypsin ( 1 mg / ml ) . - pubmed
Here , we investigated the non - ionic surfactant macrogol-260 - cetylstearylether ( TA 6 ) as an efficient vehicle for the reconstitution of G - proteins . - pubmed
Reconstitution efficiency of G - proteins was recorded by GTP gamma S - binding . - pubmed
Consumption of the product baked with a high concentration of Na - propionate significantly lowered the postprandial blood glucose and insulin responses , and significantly prolonged the duration of satiety compared with all other breads . - pubmed
When subjects consumed the breads baked with sourdough , lactic acid and Na - propionate , their glucose and insulin responses were reduced compared with the wholemeal bread alone . - pubmed
Compared to fasting with fluid and salt replacement , fasting with no fluids was associated with higher ( mean , 95 % confidence interval ) total serum cholesterol ( 8.1 % , 4.3 - 11.9 % ) , HDL cholesterol ( 7.5 % , 1.8 - 13.1 % ) , LDL cholesterol ( 10.5 % , 2.2 - 18.8 % ) , apolipoprotein A-1 ( 8.9 % , 5.0 - 12.8 % ) , and apolipoprotein B ( 10.5 % , 5.2 - 15.8 % ) . - pubmed
Here we report that clonal insulin - secreting HIT beta - cells contain substantial amounts of endogenous plasmalogens and express a similar ASCI - PLA2 activity with the following properties : ( 1 ) Enzymatic activity as well as glucose - induced eicosanoid release and insulin secretion are inhibited by a mechanism - based suicide substrate directed towards ASCI - PLA2 . - pubmed
( 2 ) HIT cell ASCI - PLA2 is selectively activated and protected against thermal denaturation by ATP . - pubmed
( 3 ) The magnitude of ASCI - PLA2 activation by the nonhydrolyzable ATP analog AMP - PCP is similar to that by ATP . - pubmed
( 4 ) The ATP concentrations required to activate ASCI - PLA2 fall within physiologic ranges in the presence of Mg2 + . - pubmed
( 5 ) ADP induces a concentration - dependent attenuation of the activation of ASCI - PLA2 by ATP . - pubmed
HIT cell ASCI - PLA2 exhibited an apparent isoelectric point of 7.5 on chromatofocusing analysis and was quantitatively adsorbed to an ATP - agarose matrix and selectively desorbed from this column by ATP . - pubmed
Mono - Q anion - exchange analysis of the active ATP - agarose eluant yielded a peak of ASCI - PLA2 activity associated with a single protein band with an apparent molecular mass of 40 kDa . - pubmed
beta 3 - AR may thus be involved in the control of lipid metabolism , possibly from fat assimilation in the digestive tract , to triglyceride storage and mobilization in adipose tissues . - pubmed
Both anti - GalCer and monoclonal antibody A2B5 , which binds polysialogangliosides , localized to mitochondria . - pubmed
The weak adsorption of the linear proinsulin and fusion protein in non - denaturing conditions , and dynamics of the SDS - protein complex 's formation in denaturing conditions were studied . - pubmed
AcSDKP inhibited the entry of progenitor cells into S phase as measured by 3H - thymidine suicide assay and the absolute number of progenitor cells with peak activity at 10 - 12 M with less activity seen at higher or lower concentrations . - pubmed
NOEs indicative of multiple and transient beta - turns around the Asn6 glycosylation site and NOEs fitting X - ray data on a linear V3 peptide - Fab complex also emerge . - pubmed
During CAP , the respiratory muscles O2 consumption might have accounted for the difference in VO2 consumption between hypoxia and normoxia . - pubmed
To optimize vascularization and repair of connective tissue , collagen solubility and glutaraldehyde crosslinking were evaluated . - pubmed
Data analysis shows that crosslinking of collagen implants with glutaraldehyde is associated ( p & lt ; 0.001 ) with detection of the implant . - pubmed
These results suggest that glutaraldehyde crosslinking of collagen implants decreases the rate of biodegradation . - pubmed
Long - term treatment with diazepam , a full allosteric modulator of the GABA ( A ) receptor , results in tolerance to its anticonvulsant effects , whereas an equipotent treatment with the partial allosteric modulator imidazenil does not produce tolerance . - pubmed
In this study , tolerance to diazepam was associated with a selective decrease ( 37 % ) in the expression of the alpha1 subunit in layers III - IV of the frontoparietal motor cortex , and a concomitant increase in the expression of the alpha5 ( 150 % ) , gamma(2L&S ) and beta(2/3 ) subunits ( 48 % ) ; an increase in alpha5 subunits was measured in all cortical layers . - pubmed
The results of this study suggest that tolerance to benzodiazepines may be associated with select changes in subunit abundance , leading to the expression of different GABA ( A ) receptor subtypes in specific brain areas . - pubmed
Protein kinases activated by sphingosine or N , N '- dimethylsphingosine , but not by other lipids , have been detected and are termed sphingosine - dependent protein kinases ( SDKs ) . - pubmed
SDK1 is a serine kinase with molecular mass 50 - 60 kDa that is strongly activated by N , N '- dimethylsphingosine and sphingosine , but not by ceramide , sphingosine 1 - phosphate , or other sphingo- , phospho- , or glycerolipids tested . - pubmed
Its activity is inhibited by the protein kinase C activator phosphatidylserine . - pubmed
The phosphorylation site was identified as Ser * in the sequence Arg - Arg - Ser - Ser *- Trp - Arg in 14 - 3 - 3 beta . - pubmed
The sigma and tau isoforms of 14 - 3 - 3 lack serine at this position , potentially explaining their lack of phosphorylation by SDK1 . - pubmed
Mutation of a lysine residue , located 9 amino acids N - terminal to the phosphorylation site , abolished 14 - 3 - 3 phosphorylation . - pubmed
The membrane form of the CD14 fusion gene , designated M14 M , contained the full - length CD14 cDNA sequence , whereas the soluble form of the fusion gene , designated M14S , was truncated to lack the sequence for the phosphatidylinositol - anchoring site . - pubmed
Spontaneous deamidation and isomerization of Asn108 in prion peptide 106 - 126 and in full - length prion protein . - pubmed
On aging , 0.8 mol of isoaspartyl residue per mole of protein was detected by the protein - l - isoaspartyl methyltransferase assay ( t(1/2 ) approximately 30 days ) . - pubmed
Cytochrome c release and caspase activation during menadione - induced apoptosis in plants . - pubmed
Western blot analysis indicated that the caspase specific inhibitors AC - DEVD - CHO ( Ac - Asp - Glu - Val - Asp - aldehyde ) and AC - YVAD - CHO ( N - acetyl - Try - Val - Ala - aspartinal ) inhibited the degradation of a caspase 3 specific substrate PARP ( poly ( ADP - ribose ) polymerase ) , and they had no effect on the release of cytochrome c. - pubmed
The fate of a dodecathymidine prodrug in cell extract was monitored by MALDI - TOF MS . - pubmed
The oligonucleotide prodrug was fully metabolized to yield the dodecathymidine phosphorothioate likely through a carboxyesterase mediated mechanism . - pubmed
CONCLUSION : Comparison of these results with those of other groups suggests that hormonal substitution with insulin and triiodothyronine in the presence of myocardial dysfunction could be of potential interest to correct myocardial dysfunction and increase the number of donor hearts . - pubmed
After six months of treatment with carteolol 2 % the MS increased significantly from 22.4 + /- 2.5 dB to 24.1 + /- 1.8 dB ( p=0.018 ) , and the mean defect ( MD ) fell from 5.3 + /- 0.8 dB to 4.7 + /- 0.6 dB ( p=0.011 ) . - pubmed
There was no significant difference in the resistance index of the CA , the OA and the CRA with the two treatments , whereas the resistance index of the SPCA dropped significantly , from 0.80 + /- 0.05 to 0.77 + /- 0.02 ( p = 0.017 ) . - pubmed
CONCLUSIONS : CDI did not show significant differences in the resistance indexes of the internal CA , the OA , and the CRA after treatment with carteolol 2 % but the resistance index of the SPCA was significantly reduced . - pubmed
Carteolol 2 % induced significant changes in the perimetric indexes examined , with an increase in MS and a decrease in MD . - pubmed
Liver acid lipase activity was lowered , while alkaline and lipoprotein lipase activities were elevated due to treatment of single dose of CCl4 . - pubmed
On the contrary alkaline , lipoprotein and hormone sensitive lipases were enhanced after 24 hr of administration of CCl4 . - pubmed
Acid lipase activity was raised by administration of different doses of abhrak bhasma concurrent with CCl4 . - pubmed
Abhrak bhasma treatment along with CCl4 enhanced alkaline lipase activity at 10 and 20 mg dose and later it was reduced at 30 and 40 mg doses and came to normal levels . - pubmed
When compared with the nonathletes , the runners had lower concentrations of plasma zinc ( p = 0.012 ) , CD3 + ( p = 0.042 ) and CD19 + lymphocytes ( p = 0.010 ) over the four weeks . - pubmed
Plasma zinc concentration correlated with total leucocyte counts in the athletes at T6 ( r = -0.72 , p & lt ; 0.05 ) and with Pokeweed mitogen stimulation in the nonathletes at T6 ( r = -0.92 , p & lt ; 0.05 ) . - pubmed
The active site region of NTE as a recombinant protein preferentially hydrolyzes lysolecithin , suggesting that this enzyme may be a type of lysophospholipase ( LysoPLA ) with lysolecithin as its physiological substrate . - pubmed
The findings indicate that tissue glutathione - dependent enzymes as well as other antioxidant enzymes function in protection against Cd2 + toxicity and that these antioxidants provide a first line of defense against Cd2 + before the induction of any metallothionein synthesis occurs . - pubmed
Soil pH had no significant effect on soil total Zn toxicity thresholds , whereas significant positive correlations were found between these thresholds and background Zn for the PNR and SIR test ( r = 0.74 and 0.71 , respectively ; log - log correlations ) . - pubmed
We first develop appropriate parameters to describe models of the redox site of rubredoxins , followed by some preliminary calculations of multinuclear iron systems of relevance to hydrogenases . - pubmed
Measurement of MMP for viable cells at the end of the exposure revealed a decrease in Rhodamine 123 uptake , which indicates a loss of MMP . - pubmed
Plasma was precipitated with acetonitrile before samples were loaded onto a zwitterionic hydrophilic interaction liquid chromatography ( ZIC - HILIC ) solid - phase extraction column . - pubmed
Improved flap viability with site - specific delivery of sildenafil citrate using fibrin glue . - pubmed
Sildenafil is a cyclic guanosine - specific phosphodiesterase type 5 ( PD-5 ) inhibitor that is widely used for erectile dysfunction . - pubmed
Potent and competitive inhibition of PD-5 enhances levels of cyclic guanosine monophosphate ( cGMP ) . - pubmed
In this study , local delivery of Sildenafil citrate with fibrin glue was accomplished to improve random flap survival . - pubmed
In Group IV ( n = 10 ) , 2.5 mg of sildenafil citrate mixed in 0.5 mL of fibrin glue was applied to donor site of the flap , whereas 10 mg of sildenafil citrate mixed in 0.5 mL of fibrin glue was applied in Group V ( n = 10 ) . - pubmed
Sildenafil and fibrin glue groups ( Group IV and V ) resulted in a statistically significant decrease in flap necrosis compared with Groups II and III ( P & lt ; 0.0001 ) . - pubmed
It acetylates the COX enzyme , irreversibly inhibiting the formation of prostaglandin . - pubmed
Cytosol , depleted of ATP - binding proteins , did not support the sufficient nuclear export of importin beta . - pubmed
The high - resolution mapping of the right atrium using a noncontact mapping system during AFL and pacing from the coronary sinus ( CS ) was performed to evaluate the conduction through the ER . - pubmed
Type III ( n = 24 , M / F = 20/4 , 61 + /- 16 years ) exhibited an activation wavefront that passed along the ER , with the sinus venosa as the posterior barrier during AFL . - pubmed
The Fc - labeled dNTPs were good substrates for DNA polymerases and were efficiently incorporated to DNA by primer extension ( PEX ) . - pubmed
Transketolase is a prominent thiamin diphosphate - dependent enzyme in sugar metabolism that catalyzes the reversible transfer of a 2 - carbon dihydroxyethyl fragment between a donor ketose and an acceptor aldose . - pubmed
coli in a covalent complex with donor ketoses d - xylulose 5 - phosphate ( X5P ) and d - fructose 6 - phosphate ( F6P ) at 1.47 A and 1.65 A resolution reveal significant strain in the tetrahedral cofactor - sugar adducts with a 25 - 30 degrees out - of - plane distortion of the C2 - Calpha bond connecting the substrates ' carbonyl with the C2 of the cofactor 's thiazolium part . - pubmed
The X - ray structure with the acceptor aldose d - ribose 5 - phosphate ( R5P ) noncovalently bound in the active site suggests that the sugar is present in multiple forms : in a strained ring - closed beta - d - furanose form in C2 - exo conformation as well as in an extended acyclic aldehyde form , with the reactive C1 aldo function held close to Calpha of the presumably planar carbanion / enamine intermediate . - pubmed
PorB(IA ) triggers efficient adherence and invasion under low phosphate conditions mimicking systemic bloodstream infections . - pubmed
However , it is not known how p53 aggravates doxorubicin ( Dox)-induced toxicity in the heart . - pubmed
Inhibition of p53 by pifithrin - alpha ( PFT - alpha ) provided different levels of protection from Dox that correlate with HSP27 levels in these cells . - pubmed
In HSF-1(+/+ ) cells , PFT - alpha attenuated Dox - induced toxicity . - pubmed
On the other hand , immunoprecipitation of p53 was found to coimmunoprecipitate HSP27 and vice versa ( confirmed by Western blotting and matrix - assisted laser desorption / ionization time of flight ) , demonstrating HSP27 binding to p53 in Dox - treated cells . - pubmed
Further analysis with flow cytometry showed that increased expression of p21 results in G(2)/M phase cell cycle arrest in Dox - treated cells . - pubmed
cTnI ( 24 kDa , pI approximately 9.5 ) contains twelve serine , eight threonine , and three tyrosine residues , which presents a challenge for unequivocal identification of phosphorylation sites and quantification of positional isomers . - pubmed
Thirty - six molecular ions of cTnI were detected in a single ESI / FTMS spectrum despite running as a single sharp band on SDS - PAGE . - pubmed
Electron capture dissociation of monophosphorylated cTnI localized two major basal phosphorylation sites : a well known site at Ser(22 ) and a novel site at Ser(76)/Thr(77 ) , each with partial occupancy ( Ser(22 ): 53 % ; Ser(76)/Thr(77 ): 36 % ) . - pubmed
Top down MS(3 ) analysis of diphosphorylated cTnI revealed occupancy of Ser(23 ) only in diphosphorylated species consistent with sequential ( or ordered ) phosphorylation / dephosphorylation of the Ser(22/23 ) pair . - pubmed
Timely levodopa ( LD ) administration prolongs survival in Parkinson ' s disease . - pubmed
Levodopa ( LD ) is the most effective drug for symptomatic control of Parkinsonism ( PS ) . - pubmed
Survival improved significantly coincident with the widespread unrestricted access to LD ( p = 0.024 - & lt ; 0.001 ) compared to pre - levodopa era cases . - pubmed
When the major disease and treatment related factors were considered the timely use of levodopa in the recent cases resulted in improved survival compared to the earlier cases who received LD later in the course of illness . - pubmed
NS-398 suppressed the expression of the survivin gene in a concentration- and time - dependent manner . - pubmed
CONCLUSIONS : Survivin was down - regulated in the growth inhibition of hepatoma cells induced by a selective COX-2 inhibitor , NS-398 , in a concentration- and time - dependent manner . - pubmed
This cAMP - dependent rpS6 kinase activity is also sensitive to PKI in vitro , and PKA exclusively phosphorylates recombinant rpS6 on Ser235 / Ser236 in vitro . - pubmed
With these data taken together , we conclude that PKA can phosphorylate rpS6 exclusively at Ser235 / Ser236 in vivo in pancreatic beta - cells , thus providing a potentially important link between cAMP signalling and the regulation of protein synthesis . - pubmed
Tetra - and pentasubstituted polyfunctional dihydropyrroles have been concisely synthesized in high yields by two different processes of the one - pot multicomponent reactions ( MCRs ) of but-2 - ynedioates 1 , amines 2 , and aldehydes 3 at room temperature or at 70 degrees C. The first one involves a domino hydroamination / nucleophilic addition / amidation - cyclization process and leads to the formation of tetrasubstituted polyfunctional dihydropyrroles 4 . - pubmed
While PEGPEI luciferase expression in vitro was approximately three times higher when compared to BPEI , a transfection rate at the level of naked DNA was observed in vivo . - pubmed
The protein contains 108 haems per molecule and 12 seryl , 12 threonyl , 35 glutamyl and 45 valyl residues to give a total number of 104 residues per molecule as N - terminal amino acids . - pubmed
Compared to untreated cells , treatment with BM - ca inhibited both the constitutively activated NF - kappaB and p38 MAPK pathways , as assessed by inhibition of both phospho - p65 and phospho - IkappaBalpha and phospho - p38 , respectively , but not the unphosphorylated forms . - pubmed
Some emerging aurora kinase inhibitors , such as VX-680 , PHA-739358 , MK-0457 and AS703569 , and Smo1 and Hedgehog ( Hh ) inhibitors promise clinical efficacy against the Bcr - Ab T315I mutant form and leukaemia stem cells , respectively . - pubmed
We suggest that the origin of the emission in Bi : GLS is Bi22- dimers . - pubmed
Gastrointestinal digestion of ovalbumin ( OVA ) was simulated using an in vitro system in two steps , which mimicked digestion in the stomach and duodenum , to assess the effect of different gastric pHs , different concentrations of proteases , and the presence of surfactants , such as phosphatidylcholine ( PC ) and bile salts ( BS ) . - pubmed
Pretreatment with MAG prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight / dry weight ratio , in total leukocyte number and neutrophil percent in the BALF , and in myeloperoxidase ( MPO ) activity of lung in dose - dependent manners . - pubmed
At the same time , pretreatment with MAG also significantly improved the super oxide dismutase ( SOD ) activity and induced the malondialdehyde ( MDA ) content in the bronchoalveolar lavage fluid ( BALF ) . - pubmed
Importantly , pretreatment with MAG prevented an increase in cyclic adenosine monophosphate - phosphodiesterase ( cAMP - PDE ) activity of lung in a dose - dependent manner . - pubmed
These results showed that anti - inflammatory effects of MAG against the LPS - induced ALI may be due to its ability of primary inhibition of cAMP - PDE activity , oxidative stress and its regulation of cytokine effects . - pubmed
Perifosine , a new synthetic alkylphospholipid Akt inhibitor , has shown anti - tumor activity by inhibition of Akt phosphorylation . - pubmed
The caspase cascade and its downstream effectors , Poly ( ADP - ribose ) polymerase ( PARP ) , were also activated simultaneously upon perifosine treatment . - pubmed
The proapoptotic effect of perifosine was in part depending on regulation of the phosphorylation level of ERK and JNK . - pubmed
Importantly , the expression of dynAP activated Akt ( also known as protein kinase B ) by promoting Ser473 phosphorylation required for the full activation , whereas knockdown of dynAP abolished this activation . - pubmed
We now describe the identification of a new ATM phosphorylation site , Thr(P)(1885 ) and an additional autophosphorylation site , Ser(P)(2996 ) , that is highly DNA damage - inducible . - pubmed
We targeted the ATM phosphorylation sites , Ser(367 ) and Ser(2996 ) , for further study by generating phosphospecific antibodies against these sites and demonstrated that phosphorylation of both was rapidly induced by radiation . - pubmed
As found for Ser(P)(1981 ) , ATM phosphorylated at Ser(367 ) and Ser(2996 ) localized to sites of DNA damage induced by radiation , but ATM recruitment was not dependent on phosphorylation at these sites . - pubmed
Phosphorylation at Ser(367 ) and Ser(2996 ) was functionally important because mutant forms of ATM were defective in correcting the S phase checkpoint defect and restoring radioresistance in ataxia - telangiectasia cells . - pubmed
PURPOSE : The aim of this study was to investigate the bone response to rough titanium implants treated with hydrofluoric acid / nitric acid ( HF / HNO3 ) solution . - pubmed
MATERIALS AND METHODS : Implants were treated with HF / HNO3 solution ( test implants ) or without HF / HNO3 solution ( control implants ) . - pubmed
Structures of 1 - Deoxy - D - Xylulose-5 - Phosphate Reductoisomerase / Lipophilic Phosphonate Complexes . - pubmed
Fosmidomycin , a potent inhibitor of 1 - deoxy - D - xylulose-5 - phosphate reductoisomerase ( DXR ) , has antibacterial and antimalaria activity . - pubmed
Here , pyridine / quinoline containing phosphonates are identified to be DXR inhibitors with IC(50 ) values as low as 840 nM. - pubmed
The crystal structure of an N - terminally truncated chaperonin reveals a monomeric protein with a fully open nucleotide binding site . - pubmed
When compared with closed state group II chaperonin structures , a large - scale 30 rotation between the equatorial and intermediate domains is observed resulting in an open nucleotide binding site . - pubmed
A protein preparation obtained from the Ehrlich ascites carcinoma ( EAC ) by acetone precipitation , water extraction and subsequent two - step alcohol fractionation reduced significantly EAC cell proliferation in vivo and in vitro . - pubmed
The ancestral allele has largely disappeared from today 's Pto populations suggesting that flagellin - triggered immunity limits pathogen fitness even in highly virulent pathogens . - pubmed
METHODS : Of 83 lamivudine - resistant patients , 49 and 34 patients harboured the rtM204I and rtM204V mutations , respectively . - pubmed
EGFR inhibitors ( gefitinib and AEE788 ) reduced clonogenicity , proliferation and survival of ependymoma - SC lines dose - dependently , and blocked EGF - induced activation of EGFR , Akt and extracellular signal - regulated kinase 1 / 2 . - pubmed
However , ex vivo treatment with AEE788 did not impair orthotopic tumor engraftment , whereas ex vivo differentiation did , suggesting that CD133 does not absolutely segregate for tumorigenicity in ependymoma - SCs . - pubmed
Accordingly , Tat decreases substrate oxidation by mitochondria isolated from these tissues , with oxygen uptake being initially restored by adding cytochrome c. - pubmed
The anion - channel inhibitor 4,4 '- diisothiocyanostilbene-2,2 '- disulfonic acid ( DIDS ) protects isolated mitochondria against Tat - induced mitochondrial membrane permeabilization ( MMP ) , whereas ruthenium red , a ryanodine receptor blocker , does not . - pubmed
NO synthase inhibitor n - nitro - l - arginine did not modify the relaxing effect provoked by AMP suggesting that the mechanism of action of this nucleotide does not involve the NO pathway . - pubmed
We recently reported that S - nitrosylation of Cdk5 ( forming SNO - Cdk5 ) at specific cysteine residues results in excessive activation of Cdk5 , contributing to mitochondrial dysfunction , synaptic damage , and neuronal cell death in models of AD . - pubmed
Here we report the identification and characterization of noncyclic nucleotide EPAC antagonists that are exclusively specific for the EPAC2 isoform . - pubmed
These EAPC2 - specific antagonists , designated as ESI-05 and ESI-07 , inhibit Rap1 activation mediated by EAPC2 , but not EPAC1 , with high potency in vitro . - pubmed
Moreover , ESI-05 and ESI-07 are capable of suppressing the cAMP - mediated activation of EPAC2 , but not EPAC1 and PKA , as monitored in living cells through the use of EPAC- and PKA - based FRET reporters , or by the use of Rap1 - GTP pull - down assays . - pubmed
Deuterium exchange mass spectroscopy analysis further reveals that EPAC2 - specific inhibitors exert their isoform selectivity through a unique mechanism by binding to a previously undescribed allosteric site : the interface of the two cAMP binding domains , which is not present in the EPAC1 isoform . - pubmed
We evaluated GC sensitivity using 2 cortisol suppression tests : a very - low - dose intravenous dexamethasone suppression test ( VLD - IV - DST ) and a low - dose oral dexamethasone suppression test . - pubmed
A positive correlation was found between the extent of cortisol suppression in vivo ( VLD - IV - DST ) and the number of days elapsed since the last flare of lupus activity . - pubmed
Amurensin G treatment induced punctate cytoplasmic expression of GFP - LC3 and increased the expression level of endogenous LC3 - II . - pubmed
Amurensin G inhibited rotenone - induced apoptosis and interfered with rotenone - induced G2 / M cell cycle arrest . - pubmed
In addition , knockdown of beclin1 , a regulator of autophagy , abolished the effect of amurensin G. - pubmed
The model predicted the existence of a UV dose threshold for the proper functioning of the PRR model , and highlighted an overlapping effect of Nucleotide Excision Repair ( the pathway effectively responsible to clean the genome from UV lesions ) on the dynamics of PCNA ubiquitylation in different phases of the cell cycle . - pubmed
A potential role for substance P and interleukin-6 in the cerebrospinal fluid of Cavalier King Charles Spaniels with neuropathic pain . - pubmed
CONCLUSION : Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in CKCSs with syringomyelia . - pubmed
Expression of Sproutys and SPREDs is decreased during lung branching morphogenesis in nitrofen - induced pulmonary hypoplasia . - pubmed
We hypothesized that expression of Sprouty2 , Sprouty4 , SPRED-1 and SPRED-2 is decreased during lung branching morphogenesis in nitrofen - induced PH . - pubmed
Immunoreactivity of Sprouty2 , Sprouty4 , SPRED-1 and SPRED-2 was markedly diminished on E18.5 in nitrofen - induced PH . - pubmed
The blood samples were collected punctually after oral administration and a specific gas chromatographic system - flame ionization detector ( GC - FID ) method was developed and employed to determine the level of borneol in the plasma . - pubmed
Diagnostic performance of FDG PET or PET / CT in prosthetic infection after arthroplasty : a meta - analysis . - pubmed
FDG PET or PET / CT are accurate methods in this setting . - pubmed
According to the XRD , TEM and XPS analysis , partially oxidated Ag NCs have been formed on the surfaces of the BET grains . - pubmed
To our knowledge , however , the effectiveness of drugs for preventing exacerbations of asthma in the autumn has not been studied previously , except for leukotriene receptor antagonists and Omalizmab . - pubmed
We further examined the involvement of intracellular calcium ions ( [ Ca2+]i ) in the ES - induced BDNF production in SCs by pharmacological studies . - pubmed
We found that the ES - induced BDNF release required calcium influx through T - type voltage - gated calcium channel ( VGCC ) and calcium mobilization from internal calcium stores , including inositol triphosphate - sensitive stores and caffeine / ryanodine - sensitive stores . - pubmed
In conclusion , ES is capable of activating SCs to secrete BDNF , which requires the involvement of calcium influx through T - type VGCC and calcium mobilization from internal calcium stores . - pubmed
Liver injury ( transaminase levels ) , mitochondrial damage ( glutamate dehydrogenase activity ) oxidative stress ( malondialdehyde levels ) , and nicotinamide adenine dinucleotide ( NAD (+ ) ) , the co - factor necessary for SIRT1 activity , were determined by biochemical methods . - pubmed
SIRT1 and its substrates ( ac - FoxO1 , ac - p53 ) , the precursor of NAD (+ ) , nicotinamide phosphoribosyltransferase ( NAMPT ) , as well as the phosphorylation of adenosine monophosphate activated protein kinase ( AMPK ) , p - mTOR , p - p70S6 K ( direct substrate of mTOR ) , autophagy parameters ( beclin-1 , LC3B ) and MAP kinases ( p - p38 and p - ERK ) were determined by Western blot . - pubmed
The elevated SIRT1 activity in presence of TMZ can be attributed to the enhanced NAMPT protein and NAD(+)/NADH levels . - pubmed
Furthermore , MAP kinases were regulated in livers preserved with IGL-1 + TMZ , as they were characterized by enhanced p - ERK and decreased p - p38 protein expression . - pubmed
CONCLUSION : Our study shows that IGL-1 preservation solution enriched with TMZ protects liver grafts from the IRI associated with OLT , through SIRT1 up - regulation . - pubmed
When lysine-9 residues in the peptides were methylated , they were protected from cleavage by endoproteinase - EndoLysC , whereas unmethylated peptides were cleaved , resulting in an increase in fluorescent intensity . - pubmed
Trichostatin A ( TSA ) is a histone deacetylase inhibitor and a potential therapeutic for various malignancies . - pubmed
TSA did not affect overall Grg1 protein levels , but instead reduced ErbB1 and ErbB2 expression , which are upregulated by Grg1 in the absence of TSA . - pubmed
We additionally found that TSA reduced the expression of VEGF and VEGFR2 , but not basic FGF and FGFR1 . - pubmed
Our findings indicate that TSA effectively inhibits Grg1 - induced lung tumorigenesis through the down - regulation of ErbB1 and ErbB2 , as well as reduced VEGF signaling . - pubmed
This suggests TSA and other HDAC inhibitors could have therapeutic value in the treatment of lung cancers with Grg1 overexpression . - pubmed
However , effects of chronic MA exposure or acute MA exposure following withdrawal on neural activation patterns within brain regions that regulate the HPA axis are unknown . - pubmed
RESULTS : ( Experiment 1 ) Compared to exposure to a single dose of MA ( 5 mg / kg ) , chronic MA exposure decreased the number of c - Fos expressing cells in the paraventricular hypothalamus , dorsomedial hypothalamus , central amygdala , basolateral amygdala , bed nucleus of the stria terminalis ( BNST ) , and CA3 hippocampal region . - pubmed
CONCLUSIONS : HPA axis - associated amygdala , extended amygdala , and hippocampal regions endure lasting effects following chronic MA exposure and therefore may be linked to stress - related withdrawal symptoms . - pubmed
OBJECTIVE : The New Delhi metallo - beta - lactamase-1 ( NDM-1 ) enzyme is a plasmid - encoded enzyme that inactivates carbapenem antibiotics . - pubmed
METHODS : Over a period of one year , all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem were subjected to MHT and conventional polymerase chain reaction ( PCR ) detection of the NDM-1 gene . - pubmed
A series of 1 - phenyl-3 / 4 -[ 4 -( aryl / heteroaryl / alkyl - piperazine1 - yl )- phenyl - urea derivatives ( 29 - 42 ) were designed , synthesized and evaluated for their anticonvulsant activity by using maximal electroshock ( MES ) , subcutaneous pentylenetetrazole ( scPTZ ) seizure tests . - pubmed
Compound 30 ( 1 -{ 4 -[ 4 -( 4 - chloro - phenyl )- piperazin-1 - yl ]- phenyl }- 3 - phenyl - urea ) exhibited marked anticonvulsant activity in MES as well as scPTZ tests . - pubmed
The phase II anticonvulsant quantification study of compound 30 indicates the ED50 value of 28.5 mg / kg against MES induced seizures . - pubmed
Liver X receptors and copper metabolism : New frontiers for the oxysterol receptors . - pubmed
OBJECTIVE : This study aimed to compare the clinical and radiologic outcomes of AO titanium locking plate and screw ( ATLPS ) and anterograde intramedullary ( AIM ) fixation for treating unstable metacarpal and phalangeal fractures . - pubmed
At 3 - month follow - up , all clinical outcomes were significantly better in the AIM than ATLPS group ( P & lt ; 0.05 ) except for VAS pain scores . - pubmed
This review focused on i ) functional interactions between homologous and heterologous pathways , ii ) methodologies applied for determining receptor endocytosis , iii ) experimental tools to determine specific endocytic routes , iv ) roles of small guanosine triphosphate - binding proteins in GPCR endocytosis , and v ) role of post - translational modification of the receptors in endocytosis . - pubmed
Basal plasma cortisol concentrations were higher at T1 compared with T2 and T3 ( p & lt ; .05 ) . - pubmed
The adrenal cortex sensitivity ( expressed as total AUC ( AUCt ) of cortisol response after ACTH application ) was lowest at T2 compared with T1 and T3 ( p & lt ; .05 ) . - pubmed
Enhancing the energy deficiency during early lactation by omission of concentrate did not affect baseline cortisol concentrations in plasma , but decreased peak height at T2 ( p & lt ; .05 ) . - pubmed
Baseline plasma cortisol concentrations were positively correlated with cortisol peak values after ACTH application , previous lactation performance , milk yield and BW ( p & lt ; .05 ) . - pubmed
Cortisol release after ACTH injection was lower in animals with high plasma concentrations of NEFA , BHB and with higher contents of fat and free fatty acids in milk ( p & lt ; .05 ) . - pubmed
Group CT was associated with a lower likelihood of being a heavy drinker 3 months later ; Group ST was associated with decreased readiness and confidence to change alcohol use . - pubmed
Facilitator speech and peer responses were related to CT and ST during the group sessions with this high risk population , which were then associated with individual changes for alcohol use . - pubmed
So , the purpose of this study was to prepare controlled - release floating ( CRF ) tablets of dipyridamole by the dry - coated method . - pubmed
Two MOF films , HKUST-1 and ZnMOF-74 , are assembled so that the oxidation of one corresponds to the reduction of the other , allowing the two sides of the device to simultaneously change color . - pubmed
These MOF films exhibit cycling stability unrivaled by other MOFs and a significant optical contrast in a lithium - based electrolyte . - pubmed
HKUST-1 reversibly changed from bright blue to light blue and ZnMOF-74 from yellow to brown . - pubmed
The electrochromic device associates the two MOF films via a PMMA - lithium based electrolyte membrane . - pubmed
Here we show that the serine / threonine protein kinase Sch9 branch of TORC1 signaling depends specifically on the integrity of the vacuolar membrane , and this dependency originates in changes in Sch9 localization reflected by phosphatidylinositol 3,5 - bisphosphate . - pubmed
In particular , transverse tubules ( T - tubules ) and caveolae have been found to be critical structural components for the spatial confinement of cAMP in cardiomyocytes , as exemplified by beta - adrenergic receptor ( & amp;beta ;- ARs ) signaling . - pubmed
In addition , we review the role of localized phosphodiesterase ( PDE ) activity in constraining the cAMP signal . - pubmed
High risk includes 1 ) gestational age ( GA ) & lt;=30 weeks , 2 ) birth - weight ( BW ) & lt;=1000 g , 3 ) GA 30 - 32 weeks and BW & lt;=1250 g , 4 ) born from a mother who received indomethacin for tocolysis . - pubmed
ERG ( ether - a - go - go - related gene ) channels are the members of the voltage - dependent potassium channel family , which have three subtypes , as ERG1 ( Kv 11.1 ) , ERG2 ( Kv 11.2 ) , and ERG3 ( Kv11.3 ) . - pubmed
For that reason , we aimed to study biophysical properties and physiological importance , and contribution of ERG K + currents to the formation of action potentials in the stellate and bushy neurons of the ventral cochlear nucleus ( VCN ) . - pubmed
In current clamp , ERG channel blockers , terfenadine ( 10 microM ) and E-4031 ( 10 microM ) , were applied in both cell types . - pubmed
When the lactose transporter ( Lac12 ) , de novo GDP - l - fucose pathway ( consisting of GDP - d - mannose-4,6 - dehydratase ( Gmd ) and GDP-4 - keto-6 - deoxymannose-3,5 - epimerase-4 - reductase ( WcaG ) ) , and alpha1,2 - fucosyltransferase ( FucT2 ) were introduced , the resulting engineered strain ( D452L - gwf ) produced 0.51 g / L of 2 - FL from a batch fermentation . - pubmed
In addition , 0.41 g / L of l - fucose was produced when alpha - l - fucosidase was additionally expressed in the 2 - FL producing strain ( D452L - gwf ) . - pubmed
Taken together , our study elucidated the importance of PD - L1 associated with tumor cells and non - tumor host cells , particularly BM - derived hematopoietic cells , as well as PD - L2 inducibly expressed on TAM in the suppression of anti - tumor immunity in the tumor microenvironment . - pubmed
Dexmedetomidine is a alpha2 - adrenoreceptor agonist and this drug possesses sedative , anxiolytic , analgesic , and anesthetic - sparing effect . - pubmed
Immunohistochemistry for tyrosine hydroxylase ( TH ) , 5 - hydroxytryptamine ( 5 - HT ; serotonin ) , tryptophan hydroxylase ( TPH ) and western blot for D1 dopamine receptor were also performed . - pubmed
Dexmedetomidine decreased the immobility latency and increased the expressions of TPH , 5 - HT , and D1 dopamine receptor , whereas , HT expression was decreased by dexmedetomidine treatment . - pubmed
CONCLUSION : In our results , dexmedetomidine alleviated sleep deprivation - induced depressive behaviors by increasing 5 - HT synthesis and by decreasing dopamine production with up - regulation of D1 dopamine receptor . - pubmed
GC - MS analysis revealed that , the ethyl acetate precipitation contains pyrrolo ( 1,2 - A ( pyrazine-1,4 - dione , hexahydro-3 -( 2 - methylpropyl )- ) and actinomycin C2 . - pubmed
BACKGROUND : The SWEET proteins are a group of sugar transporters that play a role in sugar efflux during a range of biological processes , including stress responses . - pubmed
FGF23 is a hormone that is mainly known as the core regulator of phosphate and vitamin D metabolism and it has been recognized as an important regulator of bone mineralization . - pubmed
In a culture study , Epo promoted BMMSC proliferation in normoxia and enhanced cell survival under the culture condition mimicking ischemia ( 1 % oxygen and nutrient deprivation ) . - pubmed
The SL / PVA / SA beads showed a network structure with a wide range of macropores . - pubmed
Compared with blank beads , the SL / PVA / SA beads showed an increased rough surface and whole distribution on the surface with the increase of SL . - pubmed
In addition , SL / PVA / SA beads have more uniform pore size , but the porosity of SL / PVA / SA beads was decreased with the increase of SL . - pubmed
The porosity of the beads was 83.24 % , 74.24 % , 71.48 % and 71.29 % for blank beads and SL / PVA / SA beads contained 30 % , 40 % and 50 % SL , when it was used as a solid carbon source for denitrification . - pubmed
Owing to their good porosity and biocompatibility , SL / PVA / SA beads had shorter acclimation time . - pubmed
After the exhaustion of carbon sources , nitrate removal rate less than 50 % occurred at the 9th , 10th and 11th day for SL / PVA / SA beads that contained 30 % , 40 % and 50 % SL , respectively . - pubmed
None of the 17 CpG sites in its DNA locus was relevant to HMMR expression . - pubmed
Binge drinking is a significant problem in adolescent populations , and because of the reciprocal interactions between ethanol ( EtOH ) consumption and the endocannabinoid ( eCB ) system , we sought to determine if adolescent EtOH intake altered the localization and function of the cannabinoid 1 ( CB1 ) receptors in the adult brain . - pubmed
Histological analyses indicated that adolescent EtOH intake significantly reduced the CB1 receptor distribution and proportion of immunopositive excitatory synaptic terminals in the medial DGML . - pubmed
Conversely , deficits in eCB - eLTD and recognition memory could be rescued by inhibiting MAGL with JZL184 . - pubmed
These findings indicate that repeated exposure to EtOH during adolescence leads to long - term deficits in CB1 receptor expression , eCB - eLTD , and reduced recognition memory , but that these functional deficits can be restored by treatments that increase endogenous 2 - arachidonoylglycerol . - pubmed
Antipsychotic Drug Trifluoperazine Suppresses Colorectal Cancer by Inducing G0 / G1 Arrest and Apoptosis . - pubmed
In vitro studies showed that TFP induced G0 / G1 cell cycle arrest to dramatically inhibit CRC cell proliferation through downregulating cyclin - dependent kinase ( CDK ) 2 , CDK4 , cyclin D1 , and cyclin E and upregulating p27 . - pubmed
Interestingly , TFP treatment increased the expression levels of programmed death-1 ligand 1 ( PD - L1 ) in CRC cells and programmed death-1 ( PD-1 ) in tumor - infiltrating CD4 + and CD8 + T cells , implying that the combination of TFP with an immune checkpoint inhibitor , such as an anti - PD - L1 or anti - PD-1 antibody , might have synergistic anticancer effects . - pubmed
We report the optical and magneto - optical properties of amorphous and crystalline Co60Fe20B20 films with thicknesses in the range of 10 nm to 20 nm characterized using spectroscopy ellipsometry ( SE ) and magneto - optical Kerr effect ( MOKE ) spectroscopy . - pubmed
Serum with various concentrations of retinol was applied to the left and right sides of the face and treated with ultrasound . - pubmed
Mild side effects appeared mainly after the first treatment and on the left side of the face with 0.5 % retinol . - pubmed
Certain SEA domains undergo intramolecular proteolysis involving a nucleophilic attack of a serine hydroxyl group on the preceding glycine carbonyl . - pubmed
Since neither a general base , a general acid , nor an oxyanion hole could be identified in MUC1 SEA , it has been suggested that proteolysis is accelerated by a non - planarity of the scissile peptide bond imposed by protein folding . - pubmed
Structural comparison with uncleaved SEA domains suggests that the number of residues evolutionarily inserted in the cleaved loop of MUC1 SEA precludes the formation of a properly hydrogen - bonded beta turn . - pubmed
In addition to various targets , THC and CBD are also well - known agonists of peroxisome proliferator - activated receptor gamma ( PPARgamma ) , a nuclear receptor involved in energy homeostasis and lipid metabolism . - pubmed
In the search of new pCBs potentially acting as PPARgamma agonists , we identified cannabimovone ( CBM ) , a structurally unique abeo - menthane pCB , as a novel PPARgamma modulator via a combined computational and experimental approach . - pubmed
CBM promoted the expression of PPARgamma target genes regulating the adipocyte differentiation and prevented palmitate - induced insulin signaling impairment . - pubmed
Tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1beta ( IL-1beta ) , malondialdehyde ( MDA ) and prostaglandin E2 ( PGE2 ) levels as well as reduced glutathione ( GSH ) content were assessed in brain homogenate at the end of the schedule . - pubmed
PTZ increased TNF - alpha , IL-1beta , MDA , and PGE2 levels meanwhile , it decreased GSH content . - pubmed
Administration of VAL , DIC , MEL , VAL + MEL and VAL + DIC decreased TNF - alpha , IL-1beta , MDA , and PGE2 levels meanwhile , they increased GSH content in the brain homogenates . - pubmed
XAV939 , an inhibitor of Wnt / beta - catenin signaling pathway , decreased GOLPH3 overexpression - induced proliferation and enhanced cisplatin - induced angiogenesis inhibition and apoptosis , which was supported by the changes of VEGF , Bax and Bcl-2 . - pubmed
Lung Adenocarcinoma Harboring Concomitant EGFR Mutations and BRAF V600E Responds to a Combination of Osimertinib and Vemurafenib to Overcome Osimertinib Resistance . - pubmed
Further , the presence of redox - active metal ions such as Cu2 + enhances the aggregation process through chelation with these Abeta42 aggregates as well as generation of Abeta42 - mediated reactive oxygen species ( ROS ) . - pubmed
This molecule consists of a natural metal - chelating tetrapeptide motif of human serum albumin ( HSA ) , a beta - sheet breaker peptide , and a sugar moiety for better bioavailability . - pubmed
Moreover , Glupep was also shown to sequester out Cu2 + from the Abeta - Cu2 + complex , reducing the ROS formation and toxicity . - pubmed
All these exciting features show Glupep to be a potent inhibitor of Abeta42 - mediated multifaceted toxicity and a prospective therapeutic lead for AD . - pubmed
Materials and Methods : Sanger sequencing and the detection of circNELL2 level after RNase R or actinomycin D treatment were performed to identify the existence of cirNELL2 in ESCC cells . - pubmed
Both NPC1 and NPC2 are proteins responsible for the exit of cholesterol from late endosomes and lysosomes ( LE / LY ) . - pubmed
Triglyceride levels were also independently associated with osteocalcin levels in premenopausal women ( beta = -0.004 [ P < 0.013 ] ) . - pubmed
The odds ratio ( OR ) for MetS was significantly higher in the lowest quartile than in the highest quartile of serum osteocalcin levels after adjusting for age , alkaline phosphatase , uric acid , high sensitivity C - reactive protein , and body mass index in all women ( OR , 2.00 ; 95 % confidence interval [ CI ] , 1.49 - 2.68 ) as well as in premenopausal ( OR , 2.23 ; 95 % CI , 1.39 - 3.58 ) and postmenopausal ( OR , 2.01 ; 95 % CI , 1.26 - 3.23 ) subgroups . - pubmed
Electron microscopic observations revealed that these hyperplastic cells originated from type II pneumoncytes . - pubmed
In the group with immediate beginning of the function of the kidney at an average WIZ of 13 minutes the creatinine clearance was significantly higher and the incidence of severe infections in the late phase was lower than in the groups with longer WIZ and very retarded beginning of the renal function . - pubmed
Glucose disappearance correlated significantly with insulin concentration . - pubmed
When arterial glucose was 10 mM , glucose disappearance during maximal insulin stimulation was fivefold greater than glucose disappearance in the absence of insulin . - pubmed
In the presence or absence of insulin , glucose disappearance was positively correlated with glucose concentration up to a glucose concentration range of 30 to 45 mM. - pubmed
When insulin ( 500 muU / ml ) was added at any glucose concentration , glucose disappearance was augmented . - pubmed
In addition to the effect of insulin on glucose uptake by the muscle cell , glucose mass action appears to be quantitatively equipotent . - pubmed
The arterial oxygen saturation of hemoglobin ( SaO2 ) measured directly with a multiwavelength spectrophotometer was compared with saturation estimated by pulse oximetry ( SpO2 ) . - pubmed
The lysosomotropic agents chloroquine and NH4Cl did not affect the increase in insulin degradation produced by transfection with dIDE , indicating that the lysosomal and IDE - mediated pathways of insulin degradation are independent . - pubmed
This release of inhibition , as indicated by increased yields of base propenal from DNA scission , is enhanced by menadione , is inhibited by superoxide dismutase , and is therefore dependent on superoxide anion . - pubmed
From each block , serial semithin sections were cut , etched , and immunostained according to the peroxidase - antiperoxidase method with well - characterized polyclonal antisera that reacted specifically with P0 glycoprotein and the basic proteins P1 and P2 . - pubmed
However , the presence of UDP - D - glucuronic acid inhibited incorporation of [ 14C ] xylose from UDP - D -[ 14C ] xylose into beta-(1 - ---4)-xylan , and neither S - adenosylmethionine nor acetyl - CoA stimulated either the xylosyltransferase or the glucuronyltransferase . - pubmed
The results indicate that the delayed cardiotoxicity induced by Dox is not mediated by an interference with the cardiac beta - adrenoceptor function . - pubmed
Thus , for these ECPs , and multiple antigen mixtures in general , the preferred immunization protocol should employ at least three hosts and utilize the cascade immunization of Thalhamer and Freund . - pubmed
Differential effects of forskolin on LH and GH release . - pubmed
The effects of forskolin , an agent which increases intracellular levels of cAMP , on basal luteinizing hormone ( LH ) and growth hormone ( GH ) release and on gonadotropin - releasing hormone ( GnRH)-stimulated LH release were documented . - pubmed
Secretory rates of both LH and GH increased in a concentration - dependent manner in response to a 1 - h challenge with 0.03 , 0.1 , 0.3 , 1 , or 3 microM forskolin . - pubmed
In response to 0.3 microM forskolin , maximum GH release was achieved within 15 - 20 min , after which secretion decreased . - pubmed
In contrast , LH release increased gradually , became maximal at 1.5 - 2 h , and remained constant until the forskolin was withdrawn . - pubmed
Cells exposed to 10 nM GnRH for 4 h exhibited a biphasic release of LH with the interphase nadir occurring at 30 min . - pubmed
The second phase of LH release was enhanced by simultaneous addition of forskolin with the GnRH . - pubmed
Whereas second phase release did not increase further , exposure of the cells to forskolin for 60 or 120 min before GnRH resulted in increased first - phase LH release . - pubmed
We suggest that , whereas our data are consistent with a role for cAMP in mediating the acute release of GH , cAMP may be involved in the process through which nonimmediately releasable LH becomes available for release . - pubmed
We have measured the length and determined the position of the alkBAC operon on this fragment by electron microscopy of R - loops . - pubmed
The enzyme preparation was applied to the affinity column in the presence of 10 mM acetylcholine to increase the affinity of CAT for coenzyme A ; the enzyme then was eluted with 10 mM acetyl coenzyme A. - pubmed
At the highest antibody concentration tested , anti - CAT 3 inhibited acetylcholine synthesis 80 % . - pubmed
Over a period of 20 months repeat cesarean sections were performed at Tripler Army Medical Center while a protocol with the following elements was used : ( 1 ) known last menstrual period ; ( 2 ) landmarks : positive urine human chorionic gonadotropin test by 6 weeks , Doppler fetal heart tone by 12 weeks , date determination by examination before 10 weeks , fetoscope fetal heart tone by 20 weeks , and date determination by size before 30 weeks ; ( 3 ) date determination by midtrimester sonogram(s ) ; ( 4 ) normal third - trimester glucose screening ; ( 5 ) biparietal diameter of 9.2 or 9.5 cm before scheduling . - pubmed
Penicillin antibodies and immunoglobulin production in newborn infants : studies with chemically modified bacteriophage . - pubmed
In vitro effect of reproterol upon pulmonary , cardiac , vascular and intestinal 3 ' , 5 '- monophosphate phosphodiesterase nucleoside . - pubmed
The blood pressure , body weight , mean 24 hour urinary electrolyte excretion , and plasma renin activity in offspring whose parents both belonged to the top third of the distribution of blood pressure in their 5 - year age - group were compared with those in offspring whose parents belonged to the bottom third . - pubmed
Cyclazocine decreased the levels of NA , and markedly increased the levels of MHPG - SP4 and 5 - HIAA in cortex , hypothalamus , midbrain and pons - medulla , while little change in 5 - HT concentration , except a decrease after the highest dose , was observed . - pubmed
Extraction of unfractionated cells with 6 M urea or solubilization in Nonidet P-40 released immunoreactive Gc protein , with physicochemical properties indistinguishable from those of Gc purified from serum ( apparent MW 56 K ; pI 4.8 - 5.1 ) . - pubmed
In addition , the theoretical expressions have been rearranged into forms suitable for determination of the relevant interaction parameters by simple graphical analysis , a procedure illustrated with results obtained in a partition equilibrium study of the NADH - dependent elution of rabbit muscle lactate dehydrogenase from Blue Sepharose . - pubmed
The bone nitrogen content remained relatively unchanged , but dry weights , DNA and collagen contents were all decreased in plaque - treated bones . - pubmed
subtilis strain Porton , PBP 2a had an altered mobility by sodium dodecyl sulfate gel electrophoresis and was present in increased amounts . - pubmed
Because the perfusion medium lacks angiotensinogen , kininogen and aldosterone , the natriuretic action in perfused kidneys may not be related to its inhibitory action on angiotensin I converting enzyme or kininase II . - pubmed
Hemoglobin connecticut ( beta 21 ( B3 ) Asp leads to Gly ): a hemoglobin variant with low oxygen affinity . - pubmed
Structural analysis of the abnormal beta - chain indicated that the amino acid substitution was at position 21 ( B3 ) , and involved the replacement of aspartic acid with glycine . - pubmed
Various GMCHA esters strongly inhibited the proteinase activity and the order of the effects was closely correlated with that on the cell growth . - pubmed
The RANTES protein causes release of histamine from basophils and is a chemoattractant for CD45RO / CD4 + " memory " T lymphocytes , monocytes , and eosinophils . - pubmed
CCK-8 - stimulated pancreatic protein output was inhibited by PP in the dorsal motor nucleus in dose - dependent and site - specific manners . - pubmed
The inhibitory effect of PP on CCK-8 - stimulated protein output was eliminated by vagotomy . - pubmed
Influence of thiols on the chlorinating effect of a myeloperoxidase system . - pubmed
Until recently mucin tandem repeat protein cores were believed to exist in random - coil conformations and to attain structure solely by the addition of carbohydrates to serine and threonine residues . - pubmed
Results of one- and two - dimensional 1H NMR correlation spectroscopy on these peptides confirm that the muc-1 protein core is not a random - coil secondary structure . - pubmed
The primary immunodominant mucin epitope , PDTRP , does not appear to attain a native conformation in the single repeat peptide ( 20 amino acids , starting with P ) , but is expressed on peptides with multiple repeats . - pubmed
Pertussis toxin ( PT ) reduced the oxytocin - stimulated formation of [ 3H]IPs in a concentration - dependent manner . - pubmed
However , a significant fraction of the oxytocin response appears to be mediated by a PT - insensitive G - protein , possibly a member of the Gq family . - pubmed
In further studies , endothelin-1 was co - infused with an inhibitor of nitric oxide production , NG - monomethyl - L - arginine , or after oral administration of the irreversible inhibitor of prostaglandin production , acetylsalicylic acid ( aspirin ) . - pubmed
Although glyceryl trinitrate partially prevented endothelin-1 - induced venoconstriction ( maximum : 33 + /- 5 % ) , inhibition of nitric oxide production did not affect endothelin-1 - induced venoconstriction ( maximum : 55 + /- 4 % ) . - pubmed
Prostacyclin was more effective at blocking the venoconstriction in response to endothelin-1 than glyceryl trinitrate ( maximum : 12 + /- 3 % ) , and there was substantial potentiation of endothelin-1 - induced venoconstriction after pretreatment with aspirin ( maximum : 90 + /- 3 % ) . - pubmed
Inhibition of 45Ca release from calvariae treated with either 1 microM hydrocortisone or 0.1 microM dexamethasone was observed with 0.01 - 30 nM salmon calcitonin ( sCT ) , 0.1 mM acetazolamide , and 0.1 mM of the bisphosphonate AHPrBP . - pubmed
Differences between our sequence data on CSAD and those reported previously were observed at two positions , which arose from a single amino acid substitution and frame shift mutation . - pubmed
Investigation of the signal transduction mechanisms induced by RANTES in T cells revealed tyrosine phosphorylation of multiple protein species with prominent bands at 70 - 85 and 120 - 130 kD. - pubmed
Expression of type II NOS by Hofbauer cells may indicate they are involved in surveillance against maternal immune insult or maternal pathogens whereby they secrete nitric oxide to exert a cytostatic / cytotoxic effect . - pubmed
This putative receptor , designated GFRalpha-3 , is closely related in amino acid sequence and is nearly identical in the spacing of its cysteine residues to both GFRalpha-1 and GFRalpha-2 . - pubmed
Fibromodulin appeared as a single polydisperse band with an apparent molecular weight ( MW ) of 80,000 ( 80 kDa ) on SDS - PAGE . - pubmed
These data suggest that fibromodulin is one of the small PGs in the PDL - matrix and may fulfill construction and maintenance functions in this tissue . - pubmed
In contrast , activation of Kir current by somatostatin was unaffected , and inhibition of calcium channels was only modestly attenuated . - pubmed
The possibility that PKC acted by phosphorylating CB1 receptors was confirmed by demonstrating that PKC phosphorylated a single serine ( S317 ) of a fusion protein incorporating the third intracellular loop of CB1 . - pubmed
Mutating this serine to alanine did not affect the ability of CB1 to modulate currents , but it eliminated disruption by PMA , demonstrating that PKC can disrupt ion channel modulation by receptor phosphorylation . - pubmed
Synthesis and potent antifolate activity and cytotoxicity of B - ring deaza analogues of the nonpolyglutamatable dihydrofolate reductase inhibitor Nalpha -( 4 - amino-4 - deoxypteroyl )- Ndelta - hemiphthaloyl - L - ornithine ( PT523 ) . - pubmed
Six new B - ring analogues of the nonpolyglutamatable antifolate Nalpha -( 4 - amino-4 - deoxypteroyl )- Ndelta - hemiphthaloy l - L - ornithine ( PT523 , 3 ) were synthesized with a view to determining the effect of modifications at the 5- and/or 8 - position on dihydrofolate reductase ( DHFR ) binding and tumor cell growth inhibition . - pubmed
The Ki for inhibition of human DHFR by the 5 - deaza and 5 - methyl-5 - deaza analogues was about the same as that of 3 ( 0.35 pM ) , 11 - fold lower than that of aminopterin ( AMT , 1 ) , and 15 - fold lower than that of methotrexate ( MTX , 2 ) . - pubmed
This trend was consistent with the published literature on the corresponding DHFR inhibitors with a glutamate side chain . - pubmed
BACKGROUND : Metallothionein ( MT ) , acting as an antioxidant and zinc binding protein , may play an important role in regulation of apoptosis . - pubmed
It is proposed that plasma PLTP mass levels are related to glucose metabolism rather than to lipid metabolism . - pubmed
We have built up a pharmacophore model of the ATP - binding site of the epidermal growth factor receptor ( EGFR ) kinase and used it for the rational design of kinase inhibitors . - pubmed
Amongst these , 4 - substituted - pyrrolo [ 2,3 - d ] pyrimidines , a new class of highly potent and selective inhibitors of the EGFR kinase , have been identified and further optimized . - pubmed
The most active derivatives inhibited the EGFR tyrosine kinase with IC50 values between 1 and 5 nM. - pubmed
In EGF - dependent cellular systems , tyrosine phosphorylation , as well as c - fos mRNA expression , was inhibited with similar IC50 values . - pubmed
Further successful application of this pharmacophore model led to the identification and optimization of phenylamino - pyrazolo [ 4,3 - d ] pyrimidines and substituted isoflavones and quinolones , other classes of potent , selective , and ATP competitive EGFR kinase inhibitors with IC50 values in the low nanomolar range . - pubmed
The aim of this study was to determine the frequency of the defective alleles in CYP2D6 and CYP2C19 in Africans and to test whether the genotype for CYP2C19 is better correlated with the proguanil / cylcoguanil ratio than the mephenytoin S / R ratio . - pubmed
HFE binds to transferrin receptor ( TfR ) , the receptor used by cells to obtain iron in the form of diferric transferrin ( Fe - Tf ) . - pubmed
The results demonstrate that HFE inhibits the TfR : Fe - Tf interaction by binding at or near the Fe - Tf binding site on TfR , and that the Fe - Tf : TfR : HFE ternary complex consists of one Fe - Tf and one HFE bound to a TfR homodimer . - pubmed
Estrogen replacement enhances acquisition of a spatial memory task and reduces deficits associated with hippocampal muscarinic receptor inhibition . - pubmed
Enhancement of vitamin - K - dependent protein function by modification of the gamma - carboxyglutamic acid domain : studies of protein C and factor VII . - pubmed
No clinically significant interactions have been reported between desloratadine and drugs that inhibit the cytochrome P450 system , nor does the drug potentiate the adverse psychomotor effects of alcohol . - pubmed
in Group I anastomosis bursting pressure ( ABP ) was by 56 + /- 17 % higher at day 2 than in controls with no pentoxifylline treatment . - pubmed
In Group II ( pentoxifylline : 2 mg/100 g , i.p . ) - pubmed
In day care center ( DCC ) SY , the penicillin resistant rate was 31.8 % , being much higher than the average level . - pubmed
However in one DCC , there could be a prevalence of some resistant clones which resulted in high penicillin resistant rate . - pubmed
A statistical analysis of published data using restricted maximum likelihood showed that the age of the bird and the dietary crude protein concentration in test diets explained most of the variation in published threonine requirements . - pubmed
Hyaluronidase , adrenaline and normal saline were injected in the subcutaneous fat . - pubmed
Results suggest that the requirement for PTTH to stimulate ecdysteroid production has been bypassed , i.e. - pubmed
The apparent Km values for NADP and D - G3P or DL - G3P were estimated to be 0.385 + /- 0.05 and 0.666 + /- 0.1 mM , respectively and the Vmax of the purified protein was estimated to be 162.5 U mg(-1 ) . - pubmed
pyogenes GAPN was markedly inhibited by sulfydryl - modifying reagent iodoacetamide , these results suggest the participation of essential sulfydryl groups in the catalytic activity . - pubmed
Studies on oxygenases ; enzymatic oxidation of imidazoleacetic acid . - pubmed
Sorbate hypersensitivity was detected in mutants of the shikimate biosynthesis pathway , strains lacking the PDR12 efflux pump or WAR1 , a transcription factor mediating stress induction of PDR12 . - pubmed
Although induction of the majority of sorbate - induced genes required Msn2p / 4p , weak acid tolerance was unaffected by a lack of Msn2p / 4p . - pubmed
Ectopic expression of PDR12 from the GAL1 - 10 promoter fully restored sorbate resistance in a strain lacking War1p , demonstrating that PDR12 is the major target of War1p under sorbic acid stress . - pubmed
A four - step purification protocol involving ammonium sulfate precipitation of a 1.0 M NaCl extract , ion - exchange chromatography on DEAE - Trisacryl M , lectin - affinity chromatography , and gel filtration chromatography resulted in electrophoretically pure GhGLP1 . - pubmed
While 1.0 M NaCl extracts from 10 - 14 DPA fiber contained superoxide dismutase and phosphodiesterase activities , GhGLP1 could be separated from both enzyme activities by the purification protocol . - pubmed
The identification and staging of these tumors can be performed through the use of MRI and/or CT scans and an octreotide scintigraphy . - pubmed
[ On the relevance of protein kinase C to lithium therapy in bipolar disorder ] . - pubmed
Valproate , which is dissimilar structurally to lithium , shares most of the effects of lithium at the level of protein kinase C ( PKC ) . - pubmed
Am80 is specific for the retinoic - acid receptor - alpha / beta , and has therapeutic effects in all - trans retinoic acid resistant acute promyelocytic leukemia . - pubmed
Am80 slightly inhibited the growth of both myeloma cells and HUVECs , and remarkably inhibited the growth of HUVECs stimulated by VEGF . - pubmed
Am80 inhibited VEGF - induced phosphorylation of VEGF receptor . - pubmed
Using CLSI methodology and disks containing cefotetan alone and in combination with 400 mug of boronic acid , 9 of 10 positive control strains and 54 of 55 AmpC - PCR - positive clinical isolates were detected . - pubmed
Boronic acid disks also enhanced detection of expanded - spectrum beta - lactamases in AmpC producers . - pubmed
The level of ribulose 1,5 - diphosphate was roughly equal in chloroplasts showing immediate linear kinetics with respect to CO ( 2 ) fixation and chloroplasts which exhibited an initial lag . - pubmed
The rapid loss of ATP was ascribed to an ATPase rather than to its utilization in kinase reactions . - pubmed
Data were obtained indicating that triose phosphates similar to pentose monophosphates and in contrast to fructose 6 - phosphate , glucose 6 - phosphate and glucose 1 - phosphate could be transported between chloroplast and suspending medium . - pubmed
In this review we will discuss pharmacoproteomic studies of drugs regulating leukemia specific targets like all - trans retinoic acid , histone deacetylase inhibitors , proteasome inhibitors and tyrosine kinase inhibitors , as well as studies on drug resistance and graft - versus - host studies during stem cell transplantations . - pubmed
Furthermore , HS induced oxidative burst , the early event of plant defense response to pathogen attack and other stress challenge ; the addition of putative inhibitors of lipoxygenase , a key enzyme for jasmonic acid biosynthesis , significantly inhibited HS - induced pacliatxel accumulation . - pubmed
When skin is directly exposed to UVR , cutaneous intracellular catalytic iron levels increase because of the release of iron from iron - binding proteins such as ferritin . - pubmed
In vivo rat brain opioid receptor binding of LY255582 assessed with a novel method using LC / MS / MS and the administration of three tracers simultaneously . - pubmed
LY255582 is a pan opioid selective receptor antagonist that has been shown to have high affinity for mu , delta , and kappa receptors in vitro . - pubmed
AIM : This study evaluated the influence of muscle mechanical afferent stimulation on the integrated arterial baroreflex control of the sinus node during dynamic exercise . - pubmed
CONCLUSION : These data suggest that the stimulation of mechanosensitive stretch receptors is capable of modifying the integrated baroreflex control of sinus node function by decreasing the cardiac vagal outflow during exercise . - pubmed
After we began oral chemotherapy with UFT - E 300 mg / day , the CEA level normalized and a CT scan revealed the liver metastasis had disappeared within about 9 months . - pubmed
The endogenous tryptamine - based agonist serotonin ( 5 - HT ; 1 ) and the benzenesulfonyl - containing tryptamine - derived 5 - HT6 receptor antagonist MS-245 ( 4a ) were automatically docked with each of the 100 receptor models using a genetic algorithm approach . - pubmed
Similar studies were conducted with the more selective 5 - HT6 receptor agonist EMDT ( 5 ) and optical isomers of EMDT - related analog 8 , as well as with optical isomers of MS-245 ( 4a)-related and benzenesulfonyl - containing pyrrolidine 6 and aminotetralin 7 . - pubmed
The results indicate that the presence or absence of an N1 - benzenesulfonyl group is a major determinant of the manner in which tryptamine - related agents bind at 5 - HT6 serotonin receptors . - pubmed
About 5 % of the egg cells and zygotes ( 2 - 3 from 50 ovules ) could be mechanically dissected within 2 h. When 0.1 % cellulase and 0.1 % pectinase were added to the mannitol isolation solution , about 18 % of the egg cells ( 8 - 10 from 50 ovules ) could be isolated within 2 h. The egg cells isolated by mechanical dissection could be used for in vitro fertilization studies without any of the potentially deleterious effects of the enzymes on the plasma membrane of egg cell . - pubmed
HTLV-1 tax - induced NF - kappaB activation is synergistically enhanced by 12 - O - tetradecanoylphorbol-13 - acetate : mechanism and implications for Tax oncogenicity . - pubmed
In assessing the possibility of modulating Tax pathogenic potential by external factors , we focused here on 12 - O - tetradecanoylphorbol-13 - acetate ( TPA ) which is a potent protein kinase C ( PKC ) activator . - pubmed
There are conflicting reports regarding the effect of TPA and PKC on NF - kappaB. - pubmed
We found that TPA promoted NF - kappaB nuclear translocation and the DNA binding of p65 dimers through PKC activation . - pubmed
However , both TPA and ectopically expressed PKC had only a marginal effect on the transcriptional competence of these dimers , indicating that the DNA binding of such dimers is insufficient by itself for gene activation . - pubmed
Notably , however , both TPA and the ectopic PKC displayed strong synergistic enhancement of the Tax - induced activation of the NF - kappaB transcriptional function . - pubmed
In contrast , TPA and the ectopic PKC only slightly elevated the low activation of the NF - kappaB transcriptional capacity by cytoplasmic Tax mutants , indicating that the nuclear translocation of Tax was essential for this synergism . - pubmed
Subsequent experiments suggested that TPA contributed to this synergism by increasing the pool of free p65 which Tax could link to CBP and elevate , thereby , the amount of a p65 - Tax - CBP ternary complex that could bind to NF - kappaB - responsive promoters and stimulate their expression . - pubmed
Leptin sensitivity of dissociated arcuate ( ARC ) and ventromedial ( VMN ) hypothalamic nucleus neurons from the 3- to 4 - wk - old offspring was assessed using fura-2 calcium imaging to monitor leptin - induced changes in intracellular calcium ( [ Ca ( 2 +)]( i ) ) as an index of neuronal activity . - pubmed
Behavior and cellular evidence for propofol - induced hypnosis involving brain glycine receptors . - pubmed
BACKGROUND : It is well documented that several general anesthetics , including propofol , potentiate glycine receptor function . - pubmed
Propofol - induced LORR was examined in the presence and absence of strychnine ( a glycine receptor antagonist ) , GABAzine ( a gamma - aminobutyric acid A receptor antagonist ) , as well as ketamine ( an antagonist of N - methyl - D - aspartic acid subtype of glutamate receptors ) . - pubmed
These results suggest that neuronal glycine receptors partially contribute to propofol - induced hypnosis . - pubmed
AIM : Alcohol in the media influences norms around use , particularly for young people . - pubmed
Plasma pre beta - HDL formation is decreased by atorvastatin treatment in type 2 diabetes mellitus : Role of phospholipid transfer protein . - pubmed
UNLABELLED : Atorvastatin lowers plasma phospholipid transfer protein ( PLTP ) activity , which stimulates pre - beta - HDL generation in vitro . - pubmed
We determined the effect of atorvastatin on pre - beta - HDL formation and its relation with PLTP activity in type 2 diabetes . - pubmed
RESULTS : Plasma pre - beta - HDL formation , triglycerides , LDL cholesterol , PLTP activity , and CETP decreased after statin treatment ( all P<0.001 vs placebo ) , whereas HDL cholesterol increased ( P<0.005 ) . - pubmed
BACKGROUND AND PURPOSE : We compared the dose - dependent reductions in cellular superoxide anion ( O ( 2 )(- ) ) by catalytic agents : superoxide dismutase ( SOD ) , polyethylene glycol ( PEG)-SOD and the nitroxide 4 - hydroxy-2,2,6,6 ,- tetramethylpiperidine-1 - oxyl ( tempol ) with uncharacterized antioxidants : 5,10,15,20 - tetrakis ( 4 - sulphonatophenyl ) porphyrinate iron ( III )( Fe - TTPS ) , ( -)- cis-3,3 ' , 4 ' , 5,7 - pentahydroxyflavane ( 2R,3R )- 2 -( 3,4 - dihydroxyphenyl )- 3,4 - dihydro-1 ( 2H )- benzopyran-3,5,7 - triol ( - epicatechin ) , 2 - phenyl-1,2 - benzisoselenazol-3 ( 2H )- one ( ebselen ) and N - acetyl - L - cysteine ( NAC ) with the spin trap nitroblue tetrazolium ( NBT ) and with the vitamins or their analogues : ascorbate , alpha - tocopherol and 6 - hydroxy-2,5,7,8 - tetramethylkroman-2 - carboxy acid ( trolox ) . - pubmed
KEY RESULTS : SOD , PEG - SOD , NAC and tempol produced a similar maximum inhibition of O ( 2 )(- ) of 80 - 90 % . - pubmed
Effectiveness in disrupted and intact cells was similar for the permeable agents , PEG - SOD and tempol , but was enhanced for SOD . - pubmed
Synthesis and evaluation of phosphopeptides containing iminodiacetate groups as binding ligands of the Src SH2 domain . - pubmed
Phosphopeptide pTyr - Glu - Glu - Ile ( pYEEI ) has been introduced as an optimal Src SH2 domain ligand . - pubmed
Peptides , Ac - K(IDA)pYEEIEK(IDA ) ( 1 ) , Ac - KpYEEIEK ( 2 ) , Ac - K(IDA)pYEEIEK ( 3 ) , and Ac - KpYEEIEK(IDA ) ( 4 ) , containing 0 - 2 iminodiacetate ( IDA ) groups at the N- and C - terminal lysine residues were synthesized and evaluated as the Src SH2 domain binding ligands . - pubmed
Fluorescence polarization assays showed that peptide 1 had a higher binding affinity ( K(d ) = 0.6 microM ) to the Src SH2 domain when compared with Ac - pYEEI ( K(d ) = 1.7 microM ) , an optimal Src SH2 domain ligand , and peptides 2 - 4 ( K(d ) = 2.9 - 52.7 microM ) . - pubmed
The binding affinity of peptide 1 to the SH2 domain was reduced by more than 2 - fold ( K(d ) = 1.6 microM ) upon addition of Ni ( 2 + ) ( 300 microM ) , possibly due to modest structural effect of Ni ( 2 + ) on the protein as shown by circular dichroism experimental results . - pubmed
The degree of fibrosis in lung was evaluated by the content of hydroxyproline ( chloramine T method ) and area of collagen ( Masson stain ) in lung . - pubmed
Impact of Y143 HIV-1 integrase mutations on resistance to raltegravir in vitro and in vivo . - pubmed
Despite this drug 's activity against viruses resistant to other antiretrovirals , failures of raltegravir therapy were observed , in association with the emergence of resistance due to mutations in the integrase coding region . - pubmed
We also describe histology and glucocorticoid receptor ( GR ) protein expression in response to inhaled NO treatment [ 40 ppm NO gas or NO donors sodium nitroprusside ( SNP ) or diethylamine NONOate ( DEA / NO ) ] . - pubmed
SNP and GC provided protection against bronchoconstriction to a similar degree in the model of severe asthma . - pubmed
Using 1 - H -[ 1,2,4 ]- oxadiazolo -[ 4,3 - a ]- quinoxalin-1 - one ( ODQ ) , a soluble guanylate cyclase inhibitor , we found this effect of NO donors to be mediated through a cGMP - independent mechanism . - pubmed
In the severe model , prolonged NO treatment restored or even increased the nuclear levels of GR . - pubmed
PF-00337210 is a potent , selective small molecule inhibitor of VEGFRs and has been under consideration for the treatment of age - related macular degeneration . - pubmed
Stress can lead to changes in the serum level of many hormones including glucocorticoids , catecholamines , growth hormone and prolactin . - pubmed
The effectiveness of UV254 reduction ( 52 - 81 % ) and colour removal ( 51 - 88 % ) was determined by the effectiveness of filtration in removing post - coagulation turbidity , as well as by the type of applied polymer . - pubmed
Estrogen - induced recruitment of TIP60 requires direct binding of TIP60 to ERalpha and the action of chromatin - remodeling ATPase BRG1 , leading to increased recruitment of histone methyltransferase MLL1 and increased monomethylation of histone H3 at Lys4 . - pubmed
Thus , complex cooperation of TIP60 with ERalpha and other chromatin - remodeling enzymes is required for estrogen - induced transcription . - pubmed
We conducted laboratory culture studies to determine the effects of three pesticides { carbaryl , glyphosate , and thiophanate - methyl [ TM ; Topsin - M(R ) ( Cerexagri - Nisso LLC ) ] } on Bd zoospore production and zoosporangia growth . - pubmed
All pesticides significantly inhibited zoospore production ; however , glyphosate and TM were more effective at doing so than carbaryl . - pubmed
Our data suggest that carbaryl and glyphosate are equally effective at inhibiting both zoosporangia and zoospore production ; however , TM is selectively toxic to zoospores but not zoosporangia . - pubmed
The objectives of the present study were : ( i ) to compare the immunohistochemical expression of estrogen , progesterone and prolactin receptors and HER-2 / neu in breast cancer before and after neoadjuvant chemotherapy ; and ( ii ) to correlate the expression of these tumor markers with partial tumor response to neoadjuvant chemotherapy . - pubmed
Additional indications are hypoxia ( S(p)O(2 ) & lt ; 90 % despite oxygen insufflation and after exclusion of tension pneumothorax ) , severe traumatic brain injury [ Glasgow Coma Scale ( GCS ) & lt ; 9 ] , trauma - associated hemodynamic instability [ systolic blood pressure ( SBP ) & lt ; 90 mmHg ] and severe chest trauma with respiratory insufficiency ( respiratory rate & gt ; 29 / min ) . - pubmed
We have previously hypothesized that mental fatigue is caused partly by neuronal brain damage through the activation of N - methyl - D - aspartate ( NMDA ) receptors by quinolinic acid ( QUIN ) , a metabolite of tryptophan ( TRP ) . - pubmed
Memantine , an NMDA receptor antagonist , inhibited not only the reduction in swimming times but also the neuronal damage induced by water - immersion . - pubmed
These results suggest that neuronal brain damage by an endogenous NMDA receptor agonist other than QUIN participates in combined fatigue by water immersion . - pubmed
This enzyme , like most lipolytic enzymes from family VIII , possesses two possible nucleophilic serines located in an S - X - X - K beta - lactamase motif and a G - X - S - X - G lipase motif . - pubmed
The serine in the S - X - X - K motif is a catalytic residue , but the role of serine within the common lipase consensus sequence G - X - S - X - G has not yet been systematically studied . - pubmed
Here , the previously reported time - intensive procedure for purification of recombinant LipBL was replaced by one - step metal - affinity chromatography purification in the presence of ATP . - pubmed
Site - directed mutagenesis performed to map the active site of LipBL revealed that mutation of serine and lysine in the beta - lactamase motif ( S(72)-M - T - K(75 ) ) to alanine abolished the enzyme activity of LipBL , in contrast to mutation of the serine in the lipase consensus motif ( S321A ) . - pubmed
Furthermore , mutagenesis was performed to understand the role of the G - X - S - X - G motif and other amino acids that are conserved among family VIII esterases . - pubmed
SLC26A9 - mediated chloride secretion prevents mucus obstruction in airway inflammation . - pubmed
BA levels were determined using proton signals ( delta(H ) 7.53 and 7.98 ) referenced to 2 - dimethyl-2 - silapentane-5 - sulfonate - d ( 6 ) sodium salt ( DSS - d(6 ) ) after simple solvent extraction from processed foods . - pubmed
pneumoniae lacks catalase , the question of how it controls H ( 2 ) O ( 2 ) levels is of critical importance . - pubmed
This study shows that tpxD encodes a functional thiol peroxidase involved in the adjustment of H ( 2 ) O ( 2 ) homeostasis in the cell . - pubmed
Kinetic experiments showed that recombinant TpxD removed H ( 2 ) O ( 2 ) efficiently . - pubmed
Mass spectrometry analysis demonstrated that TpxD Cys(58 ) undergoes selective oxidation in vivo , under conditions where H ( 2 ) O ( 2 ) is formed , confirming the thiol peroxidase activity . - pubmed
The challenge with D39 and TIGR4 with H ( 2 ) O ( 2 ) resulted in tpxD upregulation , while psaBCA expression was oppositely affected . - pubmed
However , the challenge of DeltatpxD mutants with H ( 2 ) O ( 2 ) did not affect psaBCA , implying that TpxD is involved in the regulation of the psa operon , in addition to its scavenging activity . - pubmed
NO can exert its biological function through different ways , such as the modulation of gene expression , the mobilization of second messengers , or interplays with protein kinases . - pubmed
The obtained nanostructured carbon / TiO ( 2 ) nanocomposites are characterized by X - ray diffraction ( XRD ) , high - resolution transmission electron microscopy ( HRTEM ) , UV - vis diffuse reflectance spectra ( DRS ) , Raman spectra , X - ray photoelectron spectroscopy ( XPS ) and photoluminescence spectra ( PL ) , and then are used as catalysts for photocatalytic hydrogen production . - pubmed
YM155 , a novel molecular targeted agent , suppresses survivin , which is overexpressed in many tumor types . - pubmed
Annexin V / propidium iodide staining followed by flow cytometric analysis was used to detect apoptosis in cell culture . - pubmed
Hypertension , diabetes , smoking , total cholesterol , triglycerides , LDLc , HDLc and TT genotype of the eNOS gene were independent risk factors for the development of PCAD . - pubmed
However , multiple genes are likely to contribute , either acting independently or epistatically , with the CYP2C9 - VKORC1 - warfarin test panel , an example of a clinically used gene - gene - dug interaction . - pubmed
I discuss here further instances of gene - gene - drug interactions , including a proposed dynamic effect on statin therapy by genetic variants in both a transporter ( SLCO1B1 ) and a metabolizing enzyme ( CYP3A4 ) in liver cells , the main target site where statins block cholesterol synthesis . - pubmed
ovata to induce oxidative stress in mussels exposed under laboratory conditions excepting for a certain increase of catalase , glutathione reductase and glutathione peroxidases activities , and a significantly higher capability to neutralize peroxyl radicals in mussels exposed for 14 days . - pubmed
In cell models , FTO overexpression reduced ghrelin mRNA N6 - methyladenosine methylation , concomitantly increasing ghrelin mRNA and peptide levels . - pubmed
Efficacy exposure - response relationships of the CCR5 antagonist maraviroc were evaluated across two phase III clinical trials . - pubmed
Prognostic factors for treatment success ( in decreasing order of Akaike information criterion ( AIC ) change ) were : maraviroc treatment , high - weighted overall susceptibility to background treatment , absence of an undetectable maraviroc concentration , high baseline CD4 count ( BCD4 ) , low viral load ( VL ) , race ( other than black ) , absence of non - R5 baseline tropism ( BTRP ) , and absence of fosamprenavir ( FPV ) . - pubmed
The maraviroc doses studied ( 300 or 150 mg with potent CYP3A4 inhibitors once ( q.d. - pubmed
Histone deacetylases ( HDACs ) are involved in the removal of acetyl groups from intracellular proteins . - pubmed
Namely , HOP2 - mediated strand exchange does not require ATP and , in contrast to DMC1 , joint molecules formed by HOP2 are more sensitive to mismatches and are efficiently dissociated by RAD54 . - pubmed
Dietary lycopene , angiogenesis , and prostate cancer : a prospective study in the prostate - specific antigen era . - pubmed
Because angiogenesis is a strong progression factor , an endpoint of lethal prostate cancer may be more relevant than an endpoint of indolent prostate cancer for lycopene in the era of highly prevalent prostate - specific antigen screening . - pubmed
The synthesized silver nanoparticles were characterized by UV - Vis , Infrared spectroscopy ( IR ) , Transmission Electron Microscopy ( TEM ) , X - ray Diffraction studies ( XRD ) , Nanoparticle Tracking Analysis ( NTA ) , Energy Dispersive Spectroscopy ( EDS ) , and Cyclic Voltammetry ( CV ) . - pubmed
The ethanol content was measured by a fully validated headspace - GC - FID method . - pubmed
Structural manipulation on the catecholic fragment of dopamine D ( 1 ) receptor agonist 1 - phenyl - N - methyl - benzazepines . - pubmed
The 8 - hydroxyl group , other than the 7 - hydroxyl was confirmed crucial to the interaction with the dopamine D1 receptor . - pubmed
7 -( m - Chlorophenyl ) methylamino - and 7 -( m - or o - tolyl ) methylamino - substituted benzazepines 13b - d displayed Ki values of 270 - 370 nM at the D1 receptor , which were slightly more potent than that of parent compound 1 . - pubmed
In addition , 7 -( arylmethyl ) amino - benzazepines 13a - c were found possessing high binding affinities less than 10 nM at the 5 - HT2A receptor . - pubmed
Among them , the non - substituted 7 - benzylamino analogue 13a was the most potent showing a Ki values of 4.5 nM at the 5 - HT2A receptor and a 5 - HT2A / D1 selectivity of 147 . - pubmed
AIM OF THE STUDY : The present study was designed to investigate the neuroprotective effects of rotigotine , a D3 / D2 / D1 dopamine receptor agonist , against the two powerful complex I inhibitors , 1 - methyl-4 - phenylpyridinium ( MPP + ) and rotenone , in primary mesencephalic cell culture relevant to PD . - pubmed
RESULTS : Rotigotine alone did not influence the survival of tyrosine hydroxylase immunoreactive ( THir ) neurons except at 10 microM , it significantly decreased the number of THir neurons by 40 % compared to untreated controls . - pubmed
It is mediated primarily by a developmentally regulated expression of the NKCC1 and KCC2 chloride importer and exporter respectively . - pubmed
The GABAergic depolarization acts in synergy with N - methyl - d - aspartate ( NMDA ) receptor - mediated and voltage - gated calcium currents to enhance intracellular calcium exerting trophic effects on neuritic growth , migration and synapse formation . - pubmed
The neuroprotective effect of lovastatin on MPP(+)-induced neurotoxicity is not mediated by PON2 . - pubmed
Lovastatin produced no change in paraoxonase-2 ( PON2 ) activity . - pubmed
However , studies have reported inconsistent findings regarding the association between NTs and fatty acid ( FA ) composition . - pubmed
, vanillin , alkaloids ) metabolisms , COMT genes might not be neutral markers , and represent candidate functional markers for resistance , aromatic or medicinal properties of Vanilla species . - pubmed
Metabolic syndrome was defined as & gt;=3 of the following : elevated fasting glucose , high triglycerides , low high - density - lipoprotein cholesterol , elevated systolic / diastolic blood pressure , and increased waist circumference . - pubmed
Our results indicate that : 1 ) endotoxin tolerance can be induced in astrocytes and results in TNFalpha and Cox2 mRNA induction decrease upon secondary stimulation ; 2 ) tolerance is revealed on the level of TNFalpha release and Cox2 protein expression ; 3 ) PPAR agonists GW7647 , L-165041 , and rosiglitazone control Cox2 mRNA expression levels under conditions of endotoxin tolerance . - pubmed
In particular , rosiglitazone ( a PPARgamma agonist ) induces Cox2 mRNA expression , while GW7647 ( a PPARalpha agonist ) and L-165041 ( a PPARbeta agonist ) suppress the expression . - pubmed
Aquaporin 5 ( AQP5 ) is an androgen - regulated member of a family of small hydrophobic integral transmembrane water channel proteins regulating cellular water homeostasis and growth signaling . - pubmed
Furthermore , treatment with an anti - MM agent , lenalidomide , caused ubiquitination and proteasomal degradation of Blimp-1 , leading to the de - repression of a new Blimp-1 direct target , CULLIN 4A ( CUL4A ) , and reduced Aiolos levels . - pubmed
Accordingly , lenalidomide - induced cell death was partially rescued by reintroduction of Blimp-1 or knockdown of CUL4A. - pubmed
We also showed that interruption of Blimp-1 / Aiolos regulatory pathways contributes to lenalidomide - mediated anti - MM activity . - pubmed
Sacubitril / valsartan ( formerly known as LCZ696 ) is a first - in - class angiotensin receptor neprilysin inhibitor ( ARNI ) that simultaneously suppresses RAAS activation through blockade of angiotensin II type 1 receptors and enhances vasoactive peptides including NPs through inhibition of neprilysin , the enzyme responsible for their degradation . - pubmed
Urine Concentrating Capacity , Vasopressin and Copeptin in ADPKD and IgA Nephropathy Patients with Renal Impairment . - pubmed
Copeptin and AVP increased significantly in a similar way in both groups . - pubmed
Using in vitro and in vivo SUMOylation assays , we found that JMJD2A is SUMOylated on lysine 471 by KSHV K - bZIP , a viral SUMO-2/3 - specific E3 ligase , in a SUMO - interacting motif ( SIM)-dependent manner . - pubmed
Bradykinin is responsible for angioedema attacks and is produced from contact phase activation secondary to failed C1Inh control . - pubmed
Both phenylephrine ( PE ) and norepinephrine ( NE ) are commonly used for induced hypertension , but the impact of the initial choice of vasopressor on the efficacy , adverse effects , or outcome after hemodynamic therapy for DCI is unknown . - pubmed
Furthermore , we observed an enhanced accumulation of compatible solute ( proline ) and increased levels of abscisic acid ( ABA ) and antioxidants in miR156OE genotypes . - pubmed
Consequently , we report an increased accumulation of carbon - centered radicals in cells lacking TDP1 , using electron spin resonance spectroscopy . - pubmed
Overexpression of the antioxidant enzyme superoxide dismutase 1 ( SOD1 ) reduces carbon - centered adducts and protects TDP1 - deficient cells from oxidative stress . - pubmed
Together , our data show that TDP1 resolves mtPDBs , thereby regulating mitochondrial gene transcription and oxygen consumption by oxidative phosphorylation , thus conferring cellular protection against reactive oxygen species - induced damage . - pubmed
Genes involved in mitochondrial transport , calcium uptake , mitophagy , or fission and fusion were largely dispensable for axon regrowth , with the exception of eat-3 / Opa1 . - pubmed
Surprisingly , many genes encoding components of the electron transport chain were dispensable for regrowth , except for the iron - sulfur proteins gas-1 , nduf-2.2 , nduf-7 , and isp-1 , and the putative oxidoreductase rad-8 . - pubmed
Moreover , loss of function in isp-1 reduced the enhanced regeneration caused by either a gain - of - function mutation in the calcium channel EGL-19 or overexpression of the MAP kinase DLK-1 . - pubmed
While the cellular function of RAD-8 remains unclear , our genetic analyses place rad-8 in the same pathway as other electron transport genes in axon regeneration . - pubmed
Unexpectedly , rad-8 regrowth defects were suppressed by altered function in the ubiquinone biosynthesis gene clk-1 . - pubmed
However , serotype 42 has only O - acetylation at 3 - beta - galactofuranose , an observation consistent with its loss of WciG functionality , which is associated with O - acetylation at the 2 - position and subsequent reaction with typing antiserum 35a . - pubmed
The result showed that the epitope recognized by 5D3 is located within 173 - 240 amino acid of the ALV - p27 protein . - pubmed
As a result , this study aimed to determine whether : ( a ) differences exist in re - offence rates of convicted drink - drivers between : the period between committing the drink - driving offence and licence disqualification ( pre - licence disqualification ) , during the period of licence disqualification , and after being re - licensed ( post - licence restoration ) ; and ( b ) differential effects of offence rates are evident based on Blood Alcohol Content ( BAC ) , gender , age , repeat offender status and crash involvement at the time of offence . - pubmed
The pathway for its biosynthesis , initiating with UDP - GlcNAc , requires three enzymes referred to as PglF , PglE , and PlgD. - pubmed
Specifically , PglF catalyzes the first step in the pathway , namely , the dehydration of UDP - GlcNAc to UDP-2 - acetamido-2,6 - dideoxy - alpha - d - xylo - hexos-4 - ulose . - pubmed
Most members of the SDR superfamily contain a characteristic signature sequence of YXXXK where the conserved tyrosine functions as a catalytic acid or a base . - pubmed
Strikingly , in PglF , this residue is a methionine . - pubmed
On the basis of the data reported herein , a new catalytic mechanism for a SDR superfamily member is proposed that does not require the typically conserved tyrosine residue . - pubmed
A positive correlation between the serum uric acid concentration of serum insulin ( r = 0.27 , p & lt;0.001 ) , age ( r = 0.21 , p & lt;0.002 ) and BMI patients ( r = 0.26 , p & lt;0.002 ) has been shown . - pubmed
Synthesis and Molecular Modeling Studies of N '- Hydroxyindazolecarboximidamides as Novel Indoleamine 2,3 - Dioxygenase 1 ( IDO1 ) Inhibitors . - pubmed
To discover novel IDO1 inhibitors , we designed and synthesized a series of N '- hydroxyindazolecarboximidamides . - pubmed
Among the compounds synthesized , compound 8a inhibited both tryptophan depletion and kynurenine production through the IDO1 enzyme . - pubmed
Molecular docking studies revealed that 8a binds to IDO1 with the same binding mode as the analog , epacadostat ( INCB24360 ) . - pubmed
Here , we report the synthesis and biological evaluation of these hydroxyindazolecarboximidamides and present the molecular docking study of 8a with IDO1 . - pubmed
MicroRNA-200a activates Nrf2 signaling to protect osteoblasts from dexamethasone . - pubmed
miR-200a expression activated Nrf2 signaling , which inhibited dexamethasone - induced reactive oxygen species production and OB-6 cell death / apoptosis . - pubmed
Thereafter , membranes were treated with / without TNFalpha ( 50 ng / mL ) and/or progesterone for 48 hours , harvested , and homogenized . - pubmed
RESULTS : TNFalpha increased apoptosis ( measured by caspase-3 , - 8 , and - 9 activities ) in fetal membranes , and this effect was abrogated by progesterone . - pubmed
Under basal conditions , progesterone suppressed expression of the proapoptotic protein , BID , by 0.45 ( 0.14)-fold , and increased expression of the antiapoptotic proteins , Bcl-2 and XIAP ; no change was seen in BAX or FLIP . - pubmed
In contrast , TNFalpha increased BID expression by 5.15 ( 2.92)-fold , which was prevented by pretreatment with progesterone . - pubmed
CONCLUSIONS : Progesterone inhibits apoptosis in fetal membranes by suppressing expression of the proapoptotic protein , BID ( for both basal and TNFalpha - induced apoptosis ) , and upregulating expression of the antiapoptotic proteins , XIAP and Bcl-2 ( under basal conditions only ) . - pubmed
Role of RACK1 on cell proliferation , adhesion , and bortezomib - induced apoptosis in multiple myeloma . - pubmed
The present study aimed to explore the function of RACK1 on the cell proliferation , adhesion , and bortezomib - induced apoptosis in MM . - pubmed
We also found that altered expression of RACK1 is associated with bortezomib - mediated MM cell apoptosis . - pubmed
Oestrogens control postnatal mammary gland development acting on a subset of mammary epithelial cells ( MECs ) , termed sensor cells , which are ERalpha - positive by immunohistochemistry ( IHC ) and secrete paracrine factors , which stimulate ERalpha - negative responder cells . - pubmed
To identify SH - BC-893 - induced cell signaling events that disrupt lysosomal trafficking , we compared phosphorylation profiles in cells treated with SH - BC-893 or C2 - ceramide , a non - vacuolating sphingolipid that does not impair lysosomal fusion . - pubmed
These analyses combined with functional assays uncovered the differential regulation of Akt and Gsk3b by SH - BC-893 ( vacuolating ) and C2 - ceramide ( non - vacuolating ) . - pubmed
Photomodulation of exogenous GFP and endogenous mitotic kinesin-5 ( Eg5 ) gene expression in cells was achieved using the prepared caged Dex- p - siRNA nanoparticles . - pubmed
Seasonal variation of arsenic and antimony in surface waters of small subarctic lakes impacted by legacy mining pollution near Yellowknife , NT , Canada . - pubmed
Moreover , WGLSF effectively reduced the hepatic levels of free fatty acids and the pro - inflammatory cytokines TNF - alpha , IL-6 , and NF - kappaB. - pubmed
Intracellular ATP Delivery Causes Rapid Tissue Regeneration via Upregulation of Cytokines , Chemokines , and Stem Cells . - pubmed
Conversely , the wounds treated with ATP - vesicles showed significantly higher expression of MCP-1 and stem cell markers ( CD44 , CD106 , CD146 , and CD34 ) at day 1 , significantly higher IL-1beta and TNF - alpha expression from day 1 - 4 , and significantly higher VEGF - A , VEGF - D , and VEGFR-2 expression from day 4 - 6 when compared to the controls . - pubmed
Structural modifications of compound 2 , an angiokinase inhibitor reported by our group were performed , which led to the discovery of methyl ( Z )- 3 -((( 4 -( 2 - methyl-5 -(( 4 - methylpiperazin-1 - yl ) methyl )- 1H - pyrrol-1 - yl ) phenyl ) amino )( phenyl ) methylene )- 2 - oxoindoline-6 - carboxylate ( 7h ) . - pubmed
Based upon the results of spectroscopic analyses and ECD measurements , the structures of new compounds were determined to be the 6 - 6'- ( 1 and 2 ) and 1 - 5 '- ( 3 ) C - C connected pseudo - dimeric anthraquinones , respectively . - pubmed
We created a critical illness severity scoring system ( CISSS ) using age , comorbidities , heart rate , mean arterial blood pressure , temperature , respiratory rate , hematocrit , white blood cell count , creatinine , sodium , glucose , albumin , bilirubin , bicarbonate , use of invasive mechanical ventilation , and whether the admission was surgical or not . - pubmed
The engineered FN / PNIPAM DAE system significantly increased the symmetric renewal of ADMSCs , particularly between passages 7 and 9 ( p7 - p9 ) , before it dropped down to the level of the control ( FN - coated TC polystyrene ) . - pubmed
FN supported also the osteogenic response of ADMSCs ( apart from the previous observations with plain PNIPAM substrata ) indicated by the significant increase of alkaline phosphatase ( ALP ) activity at days 7 and 14 . - pubmed
The femoral artery contractile changes induced by the extracellular nucleotides and dinucleotide were similar when nitric oxide synthase was inhibited . - pubmed
RIPK1 is involved in mediating the interaction between AMPK and TSC2 and facilitate TSC2 phosphorylation at Ser1387 . - pubmed
RIPK1 - deficient cells are unable to cope with energetic stress and are vulnerable to low glucose levels and metformin . - pubmed
The post - mineralogy analysis of mineral dust proxies added to ASW showed a diatom - induced increased formation of goethite , where the amount of goethite formed correlated with observed enhanced growth . - pubmed
, triolein , elastin , keratin , fibrillar collagen , and type IV collagen ) associated with primary NPC and post - treatment recurrent NPC tissue compared to normal nasopharyngeal tissue . - pubmed
To understand AMZ action , glutathione content , superoxide dismutase enzyme activity , and lipid peroxidation were evaluated in CC . - pubmed
Moreover , cytidine deaminase expression was significantly increased in the CTC lines obtained after failure of the first- and second - line 5 - fluorouracile - based treatments , suggesting that these CTCs can eliminate this specific drug and resist to therapy . - pubmed
Abdominal computed tomography ( CT ) was examined after completion of 8 cycles of adjuvant chemotherapy with capecitabine combination oxaliplatin . - pubmed
Effects of adaptation , prolactin , cortisol and actinomycin D on plasma osmotic balance and ( Na +- K +) ATPase in gill and kidney . - pubmed
Albumin , R4 , R2 , and R1 all bound to an affinity column for albumin ( Cibacron blue 3G - A dye coupled to agarose ) and were eluted in that order by a KSCN gradient . - pubmed
It was not possible to incorporate labeled amino acid into albumin or the albumin - like R proteins with pituitary incubates , indicating that albumin - like proteins were not synthesized de novo by pituitary glands . - pubmed
The function of these endogenous albumin fragments is unknown , but albumin fragments produced in vitro by other investigators are known to potentiate bradykinin , a hypotensive peptide . - pubmed
Conformational analysis of ApA , ApC , CpA , CpC , GpG ] . - pubmed
The quinazoline couterpart of folic acid ( 5,8 - deazafolic acid ) as well as its 10 - methyl analogue has been shown to be an effective inhibitor of thymidylate synthetase from several different sources . - pubmed
Mixtard , Penmix and Actrapid were the best understood insulins , Insulatard , Humulin Zn and Protaphane the worst . - pubmed
Low flow - rates and small particle diameters maximise the displacements , as well as improve the resolution between the parent insulin and its desamido contaminant . - pubmed
The gradient slope did not substantially affect the separation between the parent insulins , but , due to the relative " S " values of the solutes , an increase in gradient slope improved the separation of the parent insulin from its desamido compound . - pubmed
The values of catalytic characteristics of the modified form in tRNA(Tyr ) aminoacylation reaction ( Km = 1.19 microM and kcat = 2.99 min-1 ) are close to those obtained for the main form of the enzyme ( 0.69 microM and 2.97 min-1 , respectively ) . - pubmed
Amino acid composition of the low - molecular form of tyrosyl - tRNA - synthetase has been determined . - pubmed
A proteolytically modified form of tyrosyl - tRNA - synthetase possesses , like the main form , the affinity to high - molecular rRNA but it is eluted from the column filled with rRNA - sepharose at lower salt concentration ( 50 mM KCl ) as compared to the main form of the enzyme ( 100 mM KCl ) . - pubmed
1H resonance assignment and secondary structure determination of the dimerization domain of transcription factor LFB1 . - pubmed
It is shown that partial ( 85 - 96 % ) inhibition with any one of three inhibitors is effective when the inhibitor is added in G1 or in S , but more complete ( 99 % ) inhibition is effective only if the inhibitor is added after cells have entered S. In addition , cells begin to respond to the inhibition after a lag that increases as DNA synthesis in the early part of S is progressively inhibited with aphidicolin , indicating that competence to respond is achieved only after cells have reached a particular point in the replication of their genome . - pubmed
Within the first 20 days after MMI withdrawal the increase of serum T3 levels was correlated with the observed decrease of serum TSH concentrations . - pubmed
Six months after MMI withdrawal basal serum TSH concentration was normal while an exaggerated response to TRH was observed . - pubmed
Monovalent cations ( Na + , Li + , and Cs + ) , divalent cations ( Mg2 + , Ca2 + , and Mn2 + ) , and guanine nucleotides did not significantly affect the specific binding of [ 3H ] ICI-198615 to guinea pig lung LTD4 receptors . - pubmed
MeSH : D 12.776.543.750.830.630 Receptors , estrogen G 6.184.154.803.817.349 Receptors , estrogen G 12.803.817.349 Receptors , estrogen D 12.776.543.750.830.700 Receptors , progesterone G 6.184.154.803.817.777 Receptors , progesterone G 12.803.817.777 Receptors , progesterone A 5.360.319.114.170 Broad ligament A 5.360.319.114.803 Round ligament - pubmed
The 5'-terminal triphosphate , present on the primary transcript , remains intact through 3'-terminal maturation and through subsequent transport of the tRNA to the cytoplasm . - pubmed
A reagent and a derivatization procedure have been developed which result in trimesylation of all - OH and -NH2 groups on proteins : serine , threonine , tyrosine , and lysine side chains are all completely derivatized . - pubmed
Conditions for solubilization of proteins in anhydrous organic solvents for the derivatization are described ; removal of blocking groups results in the polar , highly water - soluble protein derivative which behaves as a monomer during gel permeation chromatography in simple aqueous buffers even in the absence of detergents . - pubmed
The octapeptide Thr - Pro - Pro - Thr - Pro - Ser - Pro - Ser , which occurs between residues 233 and 240 , has an IC50 value of 0.26 mM for the type 1 proteinase and 0.50 mM for the type 2 enzyme . - pubmed
Acetylation of the octapeptide N - terminal amino group lowers affinity for the type 1 proteinase sixfold but does not change binding to the type 2 enzyme . - pubmed
Amidation of the C - terminal carboxyl group does not change binding to the type 1 proteinase but improves IC50 for the type 2 enzyme . - pubmed
Simultaneous blockade of both the N- and C - termini drastically lowers affinity of the octapeptide for both proteinases . - pubmed
Sequential replacement of the hydroxy amino acids in the blocked octapeptide with cysteine yields a series of inhibitors that generally bind to the neisserial IgA1 proteinases as well as or better than the unblocked octapeptide . - pubmed
The most effective inhibitor contains a cysteine residue at position 6 ( P3 ' ) and has an IC50 value for the type 2 IgA1 proteinase of 50 microM. - pubmed
The N terminus of the SulA protein [ amino acid ( aa ) residues 1 - 30 ] has diverged extensively during the evolution of Enterobacteriaceae , whereas the central region ( aa residues 31 - 149 ) has been well conserved . - pubmed
Serum proteins are precipitated by the addition of 30 microliters of 40 % trichloroacetic acid solution . - pubmed
Competition experiments indicate a substrate specificity of specific 3H - Ro 5 - 4864 binding similar to the properties already demonstrated for the specific binding of this ligand to peripheral benzodiazepine binding sites in many other tissues . - pubmed
The carbohydrate moieties exposed on enterobacteria before and after antibody binding have been tested with fluorescent lectins . - pubmed
typhimurium MS and R 10 enhanced the affinity for RCA - I ( Gal ) and to a smaller extent for WGA ( GlcNAc ) which may be explained by exposure of IgG oligosaccharide . - pubmed
The corresponding sugars N - acetylglucosamine , mannose and glucose are present in the carbohydrate chain of the secretory component as well as in IgA indicating that when SIgA antibody binds its sugar components are exposed . - pubmed
The assay used a alpha - naphthyl acetate as substrate and measured the combined activity of the enzymes acetylcholinesterase ( AChE , E.C. - pubmed
The effect of increased hydrophobicity at position 6 of luteinizing hormone - releasing hormone ( LH - RH ) has been investigated by the incorporation of a series of 15 very hydrophobic , unnatural D - amino acids at this position . - pubmed
Parallel assessment by the Scatchard method suggests , as expected , that albumin is the principle protein moiety responsible for binding piretanide . - pubmed
The antibody titer measured for the 5 - O - acetyl-6 - O - stearoyl compound 9 was comparable to that obtained with 6 - O - stearoyl - MDP at 50 micrograms , and both compounds were more active than MDP at 5 micrograms . - pubmed
Other molecules , including angiotensin II , bradykinin , thrombin , and catecholamines , are beginning to appear to play major roles in control of smooth - muscle replication in vivo as well . - pubmed
In agreement with previous reports , our deletion analysis indicates that a region encompassing amino acids 411 to 419 ( KRLLSEKKT ) in the cytoplasmic domain of CD4 was required to confer Vpu sensitivity . - pubmed
However , six specific substitution mutations within this region did not confer CD4 resistance to Vpu , suggesting that neither the amino acid sequence nor the charge of the amino acids in this region was critical to Vpu - induced CD4 degradation . - pubmed
A dileucine motif that is important for internalization of CD4 and Nef - induced CD4 down - regulation was also not required for Vpu - induced CD4 degradation . - pubmed
These events coincide with the ability of GM - CSF to increase the intracellular levels of both cyclic 3 ' , 5 '- adenosine monophosphate ( cAMP ) and cyclic 3 ' , 5 '- guanosine monophosphate ( cGMP ) in T cells , to increase the binding of ( gamma-35S ) GTP to T - cell membranes , and to enhance GTPase activity as determined by increased hydrolysis of 32P - GTP . - pubmed
IL-2 also induced IL-2R expression , cyclic nucleotide secretion , and G - protein activation . - pubmed
Furthermore , GTP binding and GTPase activity induced by GM - CSF or IL-2 were inhibited by the addition of cholera toxin ( CT ) , but not pertussis toxin ( PT ) . - pubmed
Coumermycin A1 is an inhibitor of DNA gyrase , an enzyme that catalyzes supercoiling of DNA and is required for bacterial DNA replication . - pubmed
burgdorferi was more susceptible than many other eubacteria to coumermycin as well as novobiocin , another coumarin antibiotic ; this contrasted with its relative resistance to the DNA gyrase inhibitors nalidixic acid , oxolinic acid , and ciprofloxacin . - pubmed
In contrast , WEB 2086 , a platelet - activating factor antagonist , had no effect on lung albumin leak . - pubmed
Inducible , Ca ( 2 +)- independent nitric oxide ( NO ) synthase activity in the heart is elevated during endotoxemia and the resulting excess release of NO depresses cardiac contractile function . - pubmed
High affinity 3H - dopamine ( DA ) and 14C GABA uptakes as well as immunocytochemical staining of tyrosine hydroxylase ( TH)-containing cells were used as specific functional markers of DA and GABA neuron viability . - pubmed
The presence of increased apoptosis was verified by ultrastructural and terminal transferase - mediated dUTP nick end labeling analyses . - pubmed
By 3 DIV , synapsin - positive puncta ( previously shown to correspond to presynaptic specializations ) were detected surrounding the soma and proximal dendritic processes , whereas comparable aggregations of synelfin did not appear until several days later . - pubmed
Genome segment 10 of CfCPV was found to be 1171 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 258 residues ( Mr of 29,795 ) , consistent with an apparent Mr of 30.5 kDa determined by SDS - PAGE of purified polyhedrin . - pubmed
Analysis of HIV-1 reverse transcriptase gene mutations in infected children treated with zidovudine . - pubmed
After 15 min of UV action form of the humoral activity products of POL was increased ; the redox system of vitamin E was mobilized ; the membranes proteins was opened ; the neutral lipid fraction in membranes was decreased , and its polarity was increased . - pubmed
Removal of all potential cleavage sites between the extracellular epidermal growth factor domain and the transmembrane domain by substitution and small deletions ( & lt;11 amino acid residues out of 21 ) did not significantly reduce ARIA release , whereas larger deletions abolished it . - pubmed
We propose that cleavage occurs independently of amino acid sequence at a short distance from the epidermal growth factor domain , unless sterically hindered by the nearby secondary structure . - pubmed
Fasting glucose , serum lipids and activated partial thromboplastin time , factor VIII fibrinogen , factor VII , antithrombin III , protein C , and von Willebrand factor measurements were made . - pubmed
Norepinephrine modulates single hypothalamic arcuate neurons via alpha ( 1 ) and beta adrenergic receptors . - pubmed
The results showed that : ( 1 ) of 236 neurons selected randomly and tested with NE application , 137 ( 58.0 % ) were excited , 67 ( 28.4 % ) were inhibited , and 32 ( 13.6 % ) failed to respond ; ( 2 ) substitution of low Ca ( 2 +)-high Mg ( 2 + ) artificial cerebrospinal fluid ( ACSF ) for normal ACSF abolished the NE - induced inhibitory effect but failed to abolish the excitatory effect ; ( 3 ) both the NE - induced excitatory and inhibitory effects were antagonized partly by phentolamine , prazosin , and propranolol but not by yohimbine ; ( 4 ) naloxone and glibenclamide , a blocker of adenosine triphosphate - sensitive ( K ( ATP ) ) channels , blocked the NE - induced inhibitory effect ; and ( 5 ) neurons that were inhibited by NE were also inhibited by morphine and cromakalim , an agonist of K(ATP ) channels , and moreover , the morphine - induced inhibitory effect could be blocked by glibenclamide , while the cromakalim - induced inhibitory effect was not blocked by naloxone . - pubmed
These results imply that : ( a ) NE excites arcuate neurons through a mechanism that is insensitive to lowering the extracellular Ca ( 2 + ) suggesting a direct postsynaptic response through alpha ( 1 )- and beta - adrenergic receptors , while NE inhibits cells through at least an inhibitory interneuron in arcuate and so is dependent on a Ca ( 2 +)-sensitive presynaptic release mechanism ; and ( b ) the inhibitory interneuron may be opioidergic , being excited first through alpha ( 1 )- and beta - adrenergic receptors , after which the released opioids inhibit the neurons being recorded with an involvement of activation of K(ATP ) channels . - pubmed
Response of periodontal ligament fibroblasts and gingival fibroblasts to pulsating fluid flow : nitric oxide and prostaglandin E2 release and expression of tissue non - specific alkaline phosphatase activity . - pubmed
Periodontal ligament fibroblasts , but not gingival fibroblasts , responded to fluid flow with significantly elevated release of nitric oxide and decreased expression of tissue non - specific alkaline phosphatase activity . - pubmed
Even partial suppression of prostacyclin ( without coincident inhibition of platelet COX-1 - dependent thromboxane formation ) by COX-2 inhibitors may be undesirable in acute vascular occlusive syndromes . - pubmed
The recombinant Fab fragment was eluted as a single peak in a sodium chloride gradient . - pubmed
The purified Fab57P fragment showed one band in SDS - PAGE . - pubmed
The transfection procedure has been optimized for large ( 225 - cm(2 ) ) culture flasks and F - ATM can be purified to near homogeneity as judged by SDS - PAGE . - pubmed
Human liver disease decreases methacrylyl - CoA hydratase and beta - hydroxyisobutyryl - CoA hydrolase activities in valine catabolism . - pubmed
BACKGROUND : Methacrylyl - coenzyme A ( MC - CoA ) hydratase and beta - hydroxyisobutyryl - coenzyme A ( HIB - CoA ) hydrolase are key enzymes regulating the toxic concentration of MC - CoA generated in valine catabolism . - pubmed
Similarly , cell impermeant albumin - conjugated progesterone , but not albumin - conjugated 17 beta - estradiol , decreased 5 - HT+U stimulated VMC Ca ( 2 + ) responses ( 250 + /- 34 % of basal 30 min after stimulation ) back to the prestimulation level ( 113 + /- 17 % of basal ) in 25 min ( half time = 7 min ) . - pubmed
Taken together , these experiments suggest that NPS3121 has an active mechanism to suppress the accumulation of defense transcripts and phytoalexin biosynthesis in bean . - pubmed
To investigate a role of PTEN , a tumor suppressor gene , in the regulation of hyaluronic acid ( HA)-induced invasion of glioma cells , we examined the secretion of MMP-9 in various glioma cells with or without a functional PTEN gene . - pubmed
The aim of our present study was to formulate , by the w/o / w emulsification process , LMWH microparticles as potential oral carriers prepared with biodegradable ( poly - epsilon - caprolactone and poly - lactic - co - glycolic acid ) and nonbiodegradable polycationic polymers ( Eudragit RS and RL ) , used alone or blended . - pubmed
For each formulation , LMWH released from microparticles preserved its biological activity as shown by the antifactor Xa activity . - pubmed
To achieve this goal , an anti - RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs . - pubmed
Electron microscopic images of the carp myocardium showed that the SR was observed largely as the subsarcolemmal cisternae and the reticular SR , suggesting that the RyR is localized in the junctional and corbular SR . - pubmed
CONCLUSION : The technical methods in this study may help detect the immunogenicity and immunoprotection of OmpL1 and develop more safe , highly effective rBCG bearing leptospiral antigen with long - lasting protection . - pubmed
Hemoglobin , blood cell morphology , serum protein - bound iodine , serum proteins , serum lipids and serum total cholesterol estimations , urinalyses , and agglutination studies for brucellosis were carried out . - pubmed
every 28 days for three cycles ; 2 ) anti - CD20 chimeric monoclonal antibody ( rituximab ) 375 mg / m2 i.v . - pubmed
RESULTS : In WKY , repeated administration of ivabradine produced reduction of MBP , DBP and HR , without change of systolic blood pressure ( SBP ) . - pubmed
Midazolam metabolism was also inhibited in vitro by typical chemical inhibitors of CYP3A , such as ketoconazole , erythromycin and diltiazem , and the apparent K(i ) values for ketoconazole , erythromycin and diltiazem were 0.127 , 94.2 and 29.6 microM , respectively . - pubmed
CYP3A inhibitors increased plasma midazolam concentrations when midazolam and CYP3A inhibitors were co - administered orally . - pubmed
However , the pharmacokinetic parameters of midazolam were not changed by treatment with CYP3A inhibitors when midazolam was given intravenously . - pubmed
The drug - drug interaction responsible for CYP3A enzyme(s ) inhibition was observed when midazolam and inhibitors were co - administrated orally . - pubmed
Analysis of sulfur and nitrogen odorants using solid - phase microextraction and GC - MS . - pubmed
OBJECTIVE : The only approved pharmacological approach for the symptomatic treatment of Alzheimer ' s disease in Japan is the use of a cholinesterase inhibitor , donepezil hydrochloride . - pubmed
Upon analysis of covariance , where those confounding variables ( age , sex , disease duration , education , MRI interval , APOE genotype , and baseline Alzheimer ' s Disease Assessment Scale score ) were entered into the model as covariates , the effect of donepezil treatment on hippocampal atrophy remained significant . - pubmed
In the first parts , advances in three major methods , grafting ( post - synthetic modification ) , co - condensation ( direct synthesis ) , and techniques related with periodic mesoporous organosilicates , are explained . - pubmed
Heme binding by the N - terminal fragment 1 - 44 of human growth hormone . - pubmed
The binding property is specific for the N - terminal helix of hGH , since heme binding does not occur with fragment 45 - 191 or the entire protein . - pubmed
These residues can act as specific ligands for the heme - iron , as observed with cytochromes . - pubmed
The CD4 / CD8 ratio was positively correlated with the Gln level ( r=0.704 , P<0.05 ) , while the monocyte HLA - DR expression lowered as compared to control . - pubmed
Pyruvate dehydrogenase kinase 4 ( PDK4 ) is a key regulatory enzyme involved in switching the energy source from glucose to fatty acids in response to physiological conditions . - pubmed
The enzyme may be important in glycine and serine synthesis . - pubmed
A comparison between the enzymic activity of isocitrate lyase and the amount of glycine and serine synthesized during logarithmic growth indicated that the activity was great enough to account for all of the carbon entering these amino acids during that stage of growth . - pubmed
METHODS : Arthritis was induced by immunisation with human type II collagen in complete Freund 's adjuvant and measured by footpad swelling , clinical score and histology . - pubmed
Omega-3 polyunsaturated fatty acids affect lipopolysaccharide - induced maturation of dendritic cells through mitogen - activated protein kinases p38 . - pubmed
OBJECTIVE : The omega-3 polyunsaturated fatty acids ( PUFAs ) play a key role as immune response modulators and suppressors of immunologic functions , such as lymphocyte proliferation , cytokine production , and cell surface molecular expression in T lymphocytes , monocytes , and natural killer cells . - pubmed
RESULTS : The omega-3 PUFAs reduced expression levels of costimulatory molecules CD80 and CD86 and major histocompatibility complex HLA - DR . - pubmed
IL-12 and tumor necrosis factor - alpha levels decreased significantly in the EPA and DHA groups . - pubmed
The omega-3 PUFAs significantly inhibited LPS - induced p38 phosphorylation . - pubmed
CONCLUSION : The omega-3 PUFAs may inhibit LPS - induced DC maturation and upregulate cytokine production . - pubmed
BACKGROUND / AIMS : The polyunsaturated fatty acid arachidonic acid ( AA ) has been implicated in beta - cell defence mechanisms and prostaglandin ( PG ) products of cyclooxygenase ( COX ) 2 action confer resistance to alloxan - induced apoptosis in insulin - secreting RIN cells . - pubmed
CONCLUSION : These data show that AA and its COX2 - generated metabolite , PGE ( 2 ) , can protect beta - cells from apoptosis . - pubmed
Respiratory plasticity following intermittent hypoxia : roles of protein phosphatases and reactive oxygen species . - pubmed
Here , we review accumulating evidence suggesting that the pattern - sensitivity of pLTF arises substantially from differential reactive oxygen species formation and subsequent protein phosphatase inhibition during intermittent compared with sustained hypoxia in or near phrenic motor neurons . - pubmed
Nitric oxide ( NO ) , a short - lived gaseous free radical , synthesized from L - arginine by NO synthases ( NOS ) , is a potent mediator of biologic responses involved in the pathogenesis of autoimmune rheumatic diseases , such as systemic lupus erythematosus ( SLE ) and rheumatoid arthritis ( RA ) . - pubmed
Most biological necessary NO is produced by the family of three NOS . - pubmed
We constructed a single - chain variable fragment miniantibody ( G11 - scFv ) directed toward the transactivation domain of c - Myc , which is fused with the internalization domain Int of Antennapedia at its carboxyl terminus ( a cargo - carrier construct ) . - pubmed
RESULTS : Although the methacholine threshold was related to all parameters that represent airway caliber and lability , Min%Max PEF had the strongest correlation with AHR . - pubmed
RESULTS : Levels of CEA protein and transcript were found to be higher in FUL - treated cells than in controls . - pubmed
However , when target cells were exposed to OXA before but not after FUL treatment , the up - regulation of CEA was partially inhibited . - pubmed
CONCLUSION : These results suggest that target cells must be exposed to OXA after but not before treatment with the fluoropyrimidine in order to exploit drug - induced up - regulation of CEA . - pubmed
This finding appears to provide useful information to design chemo - immunotherapy protocols based on FUL + OXA , combined with host 's immunity against CEA directed cancer vaccines . - pubmed
Aromatase inhibitors have proven to have disease - free survival advantage when used after 5 years of tamoxifen with a delay of up to 2.8 years . - pubmed
SUMMARY : In summary , at least further 4 years of aromatase inhibitor treatment after the first 5 years of adjuvant tamoxifen is recommended in postmenopausal endocrine responsive early breast cancer , nowadays . - pubmed
Treatment of various types of cells with the mitochondrial ATP - sensitive K (+ ) channel opener , diazoxide , preconditions cells to subsequent injuries and inhibits apoptosis . - pubmed
Here we demonstrate that diazoxide inhibited the proliferation of lung cancer cells as well as the transcription of cell cycle - related protein Cyclin D1 . - pubmed
Cyclin D1 overexpression inhibited the negative role of diazoxide in cell cycle progression . - pubmed
We further explored the mechanisms by which diazoxide affected Cyclin D1 transcription and found that the beta - catenin transcription factor was downregulated by diazoxide , with a corresponding decrease in Cyclin D1 promoter activity . - pubmed
O - GlcNAc modification of radial glial vimentin filaments in the developing chick brain . - pubmed
We examined the post - translational modification of intracellular proteins by beta - O - linked N - acetylglucosamine ( O - GlcNAc ) with regard to neurofilament phosphorylation in the developing chick optic tectum . - pubmed
Labeling of brain sections together with Western blot analyses showed O - GlcNAc modification of a few discrete proteins throughout development , and suggested vimentin as the protein in radial glia . - pubmed
These results indicate a regulated pattern of O - GlcNAc modification of vimentin filaments , which in turn suggests a role for O - GlcNAc - modified intermediate filaments in radial glia , but not in neurons during brain development . - pubmed
BACKGROUND : An association of the Tyr402His variant of the complement factor H ( CFH ) gene with age - related macular degeneration ( AMD ) has been shown in several Caucasian populations , while studies for an association with other single nucleotide polymorphisms ( SNP ) of complement system genes have produced inconsistent results . - pubmed
Biochemical and mechanical analysis revealed a significant increase of cell mass , 4 - hydroxyproline , collagen , and strength in the hybrid heart valve leaflets compared to controls . - pubmed
RESULTS : Adipose tissue recovery during the dynamic phase of catch - up fat is accompanied by increased adipocyte number with smaller diameter , increased expression of genes for adipogenesis and de novo lipogenesis , increased fatty acid synthase activity , increased proportion of saturated fatty acids in triglyceride ( storage ) fraction but not in phospholipid ( membrane ) fraction , and no impairment in insulin signaling . - pubmed
CONCLUSIONS : These findings suggest that increased adipose tissue insulin stimulation and consequential increase in intracellular glucose flux play an important role in initiating catch - up fat . - pubmed
Once activated , the machinery for lipogenesis and adipogenesis contribute to sustain an increased insulin - stimulated glucose flux toward fat storage . - pubmed
Rational combination therapy combines medications with complementary mechanisms of action , such as a calcium channel blocker ( CCB ) and a renin - angiotensin - aldosterone system ( RAAS ) inhibitor ; it is often more efficacious than monotherapy and allows the use of lower doses of the individual components , which usually results in improved tolerability . - pubmed
The results of histopathological examination of specimens from CB1 receptor and anandamide injected group indicate that I / R injury has regressed . - pubmed
CONCLUSION : The protective effect of endogenous anandamide on I / R injury may take place through CB2 receptors in the small intestine ; NO and prostaglandin , which are activated through the stimulation of CB2 receptors may be responsible for this protective effect ( Fig . - pubmed
After exclusion of the milk proteins , SMX and TMP were transferred to the analytical column ( an octyl column ) and the separation of the compounds from one another and from other endogenous milk components was achieved . - pubmed
This case illustrates that TBc can develop in multiple organs during adalimumab therapy , and thus , the awareness of serious complications of multiple organs and atypical extrapulmonary pattern of TBc during anti - TNF - alpha therapy needs to be increased . - pubmed
For the primary study , we tested the effects of decreasing cholinergic neurotransmission by systemic administration of the muscarinic antagonist atropine . - pubmed
The effect of ellagic acid on platelet activation as measured by the quantification of P - selectin using flow cytometry . - pubmed
We assessed the similarities of ellagic acid to known COX-2 specific and nonspecific COX inhibitors by examining their effects on platelet activation via use of P - selectin flow cytometry . - pubmed
Our findings suggest that ellagic acid is likely a nonspecific COX inhibitor . - pubmed
It also suggests that combining ellagic acid with a known nonspecific COX inhibitor such as ketorolac may cause a significant decrease in platelet activity and an increase in blood loss . - pubmed
The Plasmodium falciparum Ca ( 2 +)- ATPase PfATP6 : insensitive to artemisinin , but a potential drug target . - pubmed
Since artemisinin is a powerful anti - malarial drug which has been proposed to target a particular Ca2 + - ATPase ( PfATP6 ) in the parasite , it has been important to characterize the molecular properties of this enzyme . - pubmed
PfATP6 conserves all sequences and motifs that are important for the function and/or structure of a SERCA , such as two high - affinity Ca2 + -binding sites , a nucleotide - binding site and a phosphorylation site . - pubmed
Our data fully confirm that PfATP6 is a SERCA , but with a distinct pharmacological profile : compared with SERCA1a , it has a lower affinity for thapsigargin and much higher affinity for cyclopiazonic acid . - pubmed
Hydrogen - bonding capability of a templating difluorotoluene nucleotide residue in an RB69 DNA polymerase ternary complex . - pubmed
Here , we report crystal structure of an RB69 DNA polymerase L561A / S565G / Y567A triple mutant ternary complex with a templating dF opposite dTTP at 1.8 A - resolution . - pubmed
In this structure , direct hydrogen bonds were observed between : ( i ) dF and the incoming dTTP , ( ii ) dF and residue G568 of the polymerase , and ( iii ) dF and ordered water molecules surrounding the nascent base pair . - pubmed
Effect of tetracyclines on IgE allergic responses and asthma . - pubmed
We found that tetracyclines and chemically modified tetracyclines ( CMT ) that lack antibiotic activity prevent IgE production , making them ideal candidates for anti - allergy therapy . - pubmed
Tetracyclines significantly suppressed expression of phosphorylated p38 MAP kinase by CD4 + and CD8 + T cells but not B cells or monocytes in vivo and in vitro . - pubmed
Enzyme closure and nucleotide binding structurally lock guanylate kinase . - pubmed
The GK enzyme is subject to large conformational changes , leading from an open to a closed form , which are further influenced by the presence of nucleotides . - pubmed
Our data emphasizes the role of magnesium , highlights a water channel permitting active site hydration , and reveals a structural lock that stabilizes the closed form of the enzyme . - pubmed
Deciphering hERG channels : molecular basis of the rapid component of the delayed rectifier potassium current . - pubmed
hERG1a channels are tetramers consisting of four 1159 amino acid long proteins and have electrophysiological properties similar , but not identical , to native I(Kr ) . - pubmed
Alternative transcription of hERG1 leads to a protein with a 56 amino acid shorter N - terminus , known as hERG1b . - pubmed
Changes in tissue oxygen concentrations have been shown to produce changes in relaxation rate R1 of water . - pubmed
The aim of the study was to improve the sensitivity of oxygen enhanced R1 imaging by exploiting the higher solubility of oxygen in lipids ( as compared with water ) to sensitively monitor changes in tissue oxygen levels by selectively measuring the R1 of lipids . - pubmed
report the upregulation of IL-6 and IL-8 after the activation of the unfolded protein response ( UPR ) following exposure of melanocytes to phenols . - pubmed
Binding of IGFBP7 and IGF-1 to IGF1R was mutually exclusive , and the N - terminal 97 amino acids of IGFBP7 were important for binding to the extracellular portion of IGF1R and for preventing its activation . - pubmed
( 18 ) F - FECH - PET / CT has been proved to be an imaging agent for prostate carcinoma . - pubmed
From 18 confirmed bone involvement cases , ( 18 ) F - FECH - PET / CT identified correctly the bone involvement in 15 cases with overall sensitivity of 83.3 % . - pubmed
The lesion - related results show that the sensitivity of each investigation differs with the anatomical regions , and by comparing both results , ( 18 ) F - FECH - PET / CT was mostly superior to bone scan ; however , without a statistical significance ( P & gt ; 0.1 ) . - pubmed
In conclusion , no significant gain in sensitivity was achieved using bone scan compared with ( 18 ) F - FECH - PET / CT . - pubmed
We evaluated the carriage of chlorhexidine resistance genes and chlorhexidine susceptibility in MRSA before and after introduction of an institutional MRSA control programme incorporating chlorhexidine - based decolonization in 2004 . - pubmed
Carriage of qacA and smr and having a chlorhexidine MIC & gt;=2 mg / L did not increase overall amongst MRSA BSI isolates ; however , qacA carriage increased in CC22 compared with in CC30 ( OR , 7.21 ; 95 % CI , 1.32 - 39.17 ) . - pubmed
CONCLUSIONS : A successful infection control programme was associated with the selection of qacA linked with a higher chlorhexidine MIC in one dominant endemic MRSA clone ( CC22 ) , but not another ( CC30 ) . - pubmed
Ligand binding - induced stabilization of the Gal-7 dimer was supported by several lines of evidence : MD - based calculations of interaction energies between ligand - loaded and ligand - free states , gel filtration data and hetero - FRET spectroscopy that indicate a highly reduced tendency for dimer dissociation in the presence of lactose , CD - based thermal denaturation showing that the transition temperature of the lectin is significantly increased in the presence of lactose , and saturation transfer difference ( STD ) NMR using a molecular probe of the monomer state whose presence is diminished in the presence of lactose . - pubmed
EPR studies also allow direct identification of the monoreduced PTCDI species and spectroscopic measurements confirm the delocalization of electronic density around the carbonyl moieties of the reduced species . - pubmed
STAT pathway in the regulation of zoledronic acid - induced apoptosis in chronic myeloid leukemia cells . - pubmed
The COCs were morphologically evaluated and randomly allocated to be cultured in either a control maturation medium or supplemented with 400 ng / mL eGH , for 30 h at 38.5 C in air with 5 % CO2 . - pubmed
Compared to control , COCs incubated with eGH had : more oocytes that reached metaphase II ( 35/72 , 48.6 % vs. 60/89 , 67.4 % , respectively ; P=0.02 ) ; greater concentrations of testosterone ( 0.21 + - 0.04 vs. 0.06 + - 0.01 ng / mL ; P=0.01 ) , progesterone ( 0.05 + - 0.01 vs. 0.02 + - 0.00 ng / mL ; P=0.04 ) , and oestradiol ( 76.80 + - 14.26 vs. 39.58 + - 8.87 pg / mL ; P=0.05 ) in the culture medium , but no significant differences in concentration of androstenedione . - pubmed
A hospital based cross - sectional survey including , demographic profile , history of CKD - MBD symptoms , measurement of serum calcium , phosphate , parathyroid hormone , 25 hydroxy vitamin D ( 25 ( OH ) D ) and alkaline phosphatase ; lateral abdominal X - rays for abdominal aortic calcification ( AAC ) and echocardiography for valvular calcification ( VC ) was carried out . - pubmed
Indeed , genetic ablation of Trp53 , or pharmacological inhibition of its protein product p53 , rescues significantly the cardiovascular defects of Tbx1 heterozygous and hypomorphic mutants . - pubmed
Hexameric ATP - dependent proteases and protein remodeling machines use conserved loops that line the axial pore to apply force to substrates during the mechanical processes of protein unfolding and translocation . - pubmed
Here we demonstrate that DNAX - activating protein of 12 kDa ( DAP12 ) , a transmembrane adaptor protein that contains an immunoreceptor tyrosine - based activation motif , is a critical regulator of microglial function after nerve injury . - pubmed
The effects of structural characteristics on the chlorpyrifos microcapsule particle size , entrapment rate ( ER ) , pesticide loading ( PL ) , and release behaviors in ethyl alcohol were investigated . - pubmed
The ER and PL varied with the BA / St monomer ratio , chlorpyrifos / monomer core - to - shell ratio , and EGDMA cross - linker content with consequence that suitable PL was estimated to be smaller than 3.09 % and the highest ER was observed as 96.74 % . - pubmed
Influences of polyethylene glycol molar mass ( M PEG ) and tie line lengths ( TLL ) on purification factor ( PF ) , yields in top ( Y T ) and bottom ( Y B ) phases and partition coefficient ( K ) were evaluated . - pubmed
First scheme resulted in 65.9 % enzyme yield and PF of 2.16 in salt - enriched phase with clarified homogenate ( M PEG 1500 g mol(-1 ) , TLL 40 % ) ; Y B of 75.2 % and PF B of 2.9 with unclarified homogenate ( M PEG 1000 g mol(-1 ) , TLL 35 % ) . - pubmed
The highest PF value of integrated process was 2.26 in bottom phase ( M PEG 1500 g mol(-1 ) , TLL 40 % ) . - pubmed
Maximum response achieved was PFB = 3.3 ( M PEG 1500 g mol(-1 ) , TLL 40 % ) . - pubmed
Enzyme characterization showed G6P Michaelis - Menten constant ( K M ) equal 0.07 - 0.05 , NADP (+ ) K M 0.02 - 1.98 and optimum temperature 70 C , before and after recovery . - pubmed
Anti - fibrillation propensity of a flavonoid baicalein against the fibrils of hen egg white lysozyme : potential therapeutics for lysozyme amyloidosis . - pubmed
In vivo lysozyme fibrillation was simulated under in vitro conditions using a strong denaturant GdHCl at 3 M concentration . - pubmed
Sharp decline in the ANS fluorescence intensity compared to the partially unfolded states , almost 20 - fold increase in ThT fluorescence intensity , increase in absorbance at 450 nm suggesting turbidity , negative ellipticity peak in the far - UVCD at 217 nm , red shift of 50 nm compared to the native state in Congo red assay and appearance of a network of long rope - like fibrils in transmission electron microscope ( TEM ) analysis suggested HEWL fibrillation . - pubmed
Anti - fibrillation potency of baicalein against the preformed fibrils of HEWL was investigated following ThT assay in which there was a dose - dependent decrease in ThT fluorescence intensity compared to the fibrillar state of HEWL with the maximum effect observed at 150 - muM baicalein concentration , loss of negative ellipticity peak in the far - UVCD region , dip in the Rayleigh scattering intensity and absorbance at 350 and 450 nm , respectively , together with a reduction in the density of fibrillar structure in TEM imaging . - pubmed
The injection of helium did not change the T2 spectra of the coals . - pubmed
In contrast , the T2 spectra peaks of micro - capillary water gradually decreased and those of macro - capillary and bulk water increased with time after the injection of CO2 . - pubmed
We report herein perovskite solar cells using solution - processed silver nanowires ( AgNWs ) as transparent top electrode with markedly enhanced device performance , as well as stability by evaporating an ultrathin transparent Au ( UTA ) layer beneath the spin - coated AgNWs forming a composite transparent metallic electrode . - pubmed
Disturbances of folate metabolism due to genetic abnormalities or the presence of autoantibodies to folate receptor alpha ( FRalpha ) can impair physiologic processes dependent on folate , resulting in a variety of developmental disorders including cerebral folate deficiency syndrome and autism spectrum disorders . - pubmed
Pyrazolylamine Derivatives Reveal the Conformational Switching between Type I and Type II Binding Modes of Anaplastic Lymphoma Kinase ( ALK ) . - pubmed
Herein , we present the structure of ALK in complex with N1 -( 3 - 4 -[([ 5 -( tert - butyl )- 3 - isoxazolyl ] aminocarbonyl ) amino ]- 3 - methylphenyl-1H-5 - pyrazolyl )- 4 -[( 4 - methylpiperazino ) methyl ] benzamide ( 5a ) , a novel ALK inhibitor adopting a type II binding mode . - pubmed
At baseline and at 4 - week intervals , blood lipids , C - reactive protein , and fasting blood glucose were evaluated . - pubmed
Consumption of PTE was associated with improvements to lipid profile , including a mild reduction in cholesterol and the cholesterol : high - density lipoprotein ratio after only 4 weeks , as well as a reduction in triglycerides and very small - density lipoproteins , where average blood levels reached normal range at 8 weeks and remained within normal range for the duration of the study ( P<0.08 ) . - pubmed
No significant changes between the PTE group and the placebo group were seen for fasting glucose or C - reactive protein . - pubmed
On the other hand , the bis ( acenaphthyl ) species show a fluxional behavior , resulting in an AX to A2 spin system transition in the ( 31 ) P {( 1 ) H } variable - temperature NMR spectra . - pubmed
BACKGROUND / AIM : 5 - Aminolevulinic acid ( 5 - ALA ) , a precursor of protoporphyrin IX ( PpIX ) , is now used for photodynamic therapy ( PDT ) of pre - cancers of the skin and photodynamic diagnosis ( PDD ) of brain tumors . - pubmed
The effect of the cafeteria diet for 8 wk , which also increases adipose tissue , or conjugated linoleic acid ( CLA ) supplementation for 4 wk , a model of lipodystrophy , was evaluated 24 h after knee challenge with mBSA . - pubmed
Additional measures comprised factors of the metabolic syndrome , cortisol , relative insulin resistance , and pro - inflammatory cytokines ( interleukin-6 ; IL-6 and tumor necrosis factor - alpha , TNF - alpha ) . - pubmed
Further findings comprised increased fasting cortisol , increased TNF - alpha concentration , and decreased physical activity level in MDD compared to CTRL . - pubmed
In addition to routine biochemical analysis , fasting blood samples were studied for prothrombin time ( PT ) , activated partial thromboplastin time ( aPTT ) , fibrinogen , protein - C , protein - S , antithrombin III , factor VII , factor VIII , D - dimer , vitamin B12 , folic acid and homocysteine . - pubmed
Levels of fibrinogen , D - dimer , protein C , factor VIII , total homocystein and the number of platelets were found to be significantly higher in Crohn ' s disease patients who were in active period of the disease . - pubmed
The relationship between serum serotonin concentration and depression is well known , but there are no enough data regarding the serum change of leptin and adiponectin . - pubmed
The mean serum serotonin level was significantly lower in the group with PPD ( p = 0.001 ) , while mean serum adiponectin level was higher ( p = 0.001 ) . - pubmed
The key strategies for the construction of the molecular complexity in the targets included an asymmetric Tsuji - Trost rearrangement to set the first quaternary carbon center at C20 , an intramolecular cyclopropanation by diazo decomposition to install the second and third quaternary carbon centers at C2 and C7 , respectively , and a SmI2 -promoted acyloin condensation to assemble the isokopsine core . - pubmed
Salicylic acid ( SA ) is an important plant hormone that regulates many aspects of plant growth and development , as well as resistance to ( a)biotic stress . - pubmed
We therefore studied the influence of the surface chemistry of mesoporous silica nanoparticles ( MSNs ) carrying identical amounts of a specific antibody ( anti - B220 ) on the selectivity toward B220 - positive leukemia stem cells . - pubmed
A significant ROR value ( higher than 1 ) was only found for citalopram ( 3.35 CI95 % [ 2.90 - 3.87 ] ) or escitalopram ( 2.50 [ 2.11 - 2.95 ] ) . - pubmed
Herein , we demonstrate that the three - dimensional porous polyacrylamide - phytic acid - polydopamine ( termed as PAAM / PA / PDA ) hydrogel can be served as reusable adsorbent with high efficiency for either anionic or cationic dyes . - pubmed
Using methyl blue ( MB ) , methylene blue ( YMB ) , methyl violet ( MV ) and neutral red ( NR ) as model dyes , we investigate the effect of pH , temperature , dye concentration , and PAAM / PA / PDA hydrogel mass on the adsorption . - pubmed
Adsorption kinetic and thermodynamic analysis suggests that the dyes are adsorbed on the PAAM / PA / PDA hydrogel through the strong pi - pi stacking and anion - cation interaction , and the adsorption process satisfies a pseudo - second - order model . - pubmed
Furthermore , the free - standing PAAM / PA / PDA hydrogel can be easily removed from water after adsorption process , and regenerated by adjusting solution pH values . - pubmed
Characterization of DNA ADP - ribosyltransferase activities of PARP2 and PARP3 : new insights into DNA ADP - ribosylation . - pubmed
Poly ( ADP - ribose ) polymerases ( PARPs ) act as DNA break sensors and catalyze the synthesis of polymers of ADP - ribose ( PAR ) covalently attached to acceptor proteins at DNA damage sites . - pubmed
According to DNA substrate specificity of PARP3 and PARP2 , we propose a putative mechanistic model of PARP - catalyzed strand break - oriented ADP - ribosylation of DNA termini . - pubmed
Notably , PARP - mediated DNA ADP - ribosylation can be more effective than PARPs ' auto - ADP - ribosylation depending on the DNA substrates and reaction conditions used . - pubmed
These results suggest that certain types of complex DNA breaks can be effectively ADP - ribosylated by PARPs in cellular response to DNA damage . - pubmed
In addition , the ultrasound energy density affected the physical properties of the beverage as the size distribution of fat globule and rheological behavior , as well as the chemical properties such as antioxidant activity , ACE inhibitory activity , fatty acid profile , and volatile profile . - pubmed
Synthesis and Characterization of Pyridine Compounds for Amperometric Measurements of Leukocyte Esterase . - pubmed
Compound III had the lowest octanol / water partition coefficient ( log p=0.33 ) along with the highest topological surface area ( tPSA=222 A2 ) and the best aqueous solubility ( 4.0 mg mL-1 ) . - pubmed
UCA1 confers paclitaxel resistance to ovarian cancer through miR-129 / ABCB1 axis . - pubmed
The objective of this work was to investigate the role and molecular mechanisms of urothelial carcinoma associated 1 ( UCA1 ) in paclitaxel ( PTX ) resistance in OC . - pubmed
Collectively , our study elaborated a novel UCA1 / miR-129 / ABCB1 regulatory axis underlying PTX resistance of OC cells , providing a potential therapeutic target for OC . - pubmed
Among nonnatural amino acids , selenocysteine is the catalytic group of glutathione peroxidase ( GPx ) . - pubmed
Exposed groups were treated with the Hedgehog pathway antagonist GDC-0449 at a single dose 150 mg / kg by oral gavage from E8.5 to E15.5 . - pubmed
Post - transplant RCC was significantly associated with longer durations of pre - transplant hemodialysis ( p = 0.007 ) and post - transplant immunosuppression with cyclosporine ( p = 0.029 ) and/or mycophenolate mofetil ( p = 0.020 ) and with larger proportions of post - transplant time on mycophenolate mofetil ( p = 0.046 ) and/or prednisolone medication ( p = 0.042 ) . - pubmed
gingivalis inhibited Idh1 / 2 and Gpt1 / 2 mRNA expression , and increased Akgdh mRNA expression , thus decreasing the ratio of alpha - KG / succinate . - pubmed
Although the H2A.Bbd - H2B dimer structure largely resembles that of H2A - H2B , substitution of H2A alphaC helix residues by H2A.Bbd counterparts lead to the transition of a long alphaC - helix to the short 310 - helix , likely owing to the rearrangement of the hydrogen - bond network . - pubmed
The plsC / plsX - overexpressing ( OX + XC ) strain produced high lipid content of about 24.3%w / dcw under normal condition and was further enhanced up to 39.1%w / dcw by acetate induction . - pubmed
This OX + XC engineered strain was capable of decreasing phaA transcript level which related to poly-3 - hydroxybutyrate ( PHB ) synthesis under acetate treatment . - pubmed
Moreover , the expression level of gene transcripts revealed that the plsX- and plsC / plsX - overexpression strains had also increased accA transcript amounts which involved in the irreversible carboxylation of acetyl - CoA to malonyl - CoA. - pubmed
Mirogabalin , which is a novel ligand for the alpha2delta subunit of voltage - gated calcium channels , is under development for the treatment of pain associated with diabetic peripheral neuropathy and postherpetic neuralgia . - pubmed
Buprenorphine shows its pharmacological effects by binding to opioid receptors . - pubmed
Kinetics of the Hydrogen Abstraction PAH + OH PAH Radical + H2O Reaction Class : An Application of the Reaction Class Transition State Theory ( RC - TST ) and Structure - Activity Relationship ( SAR ) . - pubmed
A reaction class transition state theory ( RC - TST ) augmented with structure - activity relationship ( SAR ) methodology is applied to predict high - pressure limit thermal rate constants for hydrogen abstraction by OH radical from polycyclic aromatic hydrocarbons ( PAHs ) reaction class in the temperature range of 300 - 3000 K. The rate constants for the reference reaction of C6H6 + OH C6H5 + H2O is calculated by the canonical variational transition state theory ( CVT ) with small curvature tunneling ( SCT ) . - pubmed
After adjusting for potential confounders , age , living in urban areas , alcohol consumption , central obesity , elevated fasting plasma glucose level , elevated blood pressure , lower high - density lipoprotein cholesterol level and elevated triglycerides level were associated with increased risk of HUA . - pubmed
Background : Omentin , releasing by adipose - tissue may be related to glucose metabolism . - pubmed
The present study involved the measurements of oral - glucose - tolerance - test and the levels of biochemical parameters , including blood glucose , omentin , insulin , lipid - profile , as well as aminotransferases . - pubmed
The plasma omentin levels were significantly higher in type-1 diabetes group and lower in type-2 diabetes with NPD + STZ ; however , the plasma omentin levels were not changed in the HFD + STZ group . - pubmed
Conclusions : The omentin expression may be affected by insulin and glucose levels in different types of diabetes more than fat - mass , and due to the local activity , the serum omentin may not comply with its gene expression . - pubmed
AIM : Previous reports showed associations between oxytocin induced labor and mental disorders in offspring . - pubmed
We investigated the neonatal brain damage with evaluating immediate early gene expression ( c - Fos , c - Jun and JunB ) by quantitative polymerase reaction and TdT - mediated dUTP nick end labeling staining . - pubmed
To investigate the injured brain cell types , we performed double - immunostaining with TdT - mediated dUTP nick end labeling staining and each brain component specific protein , such as Oligo2 , NeuN , GFAP and Iba1 . - pubmed
Expression levels of oxytocin and Oxtr in the fetal brain were not affected by the oxytocin administered to mothers during induced labor . - pubmed
We also showed that C / Co@polyMAPTAC could reduce the cytotoxicity of the Abeta aggregate solutions . - pubmed
In the post - operative phase , therefore after the surgical treatment of mammary quadrantectomy , or mastectomy associated with homolateral sentinel lymph node removal + - axillary dissection , based on the histopathological characteristics of the tumor , the use of chemotherapy ( anthracycline + taxane ) and/or hormone therapy , whether or not associated with anti - HER2 therapy ( trastuzumab ) is the rule . - pubmed
In this work , the cytotoxic behavior of six ruthenium ( II ) complexes of stoichiometry [ ( eta6 - p - cymene ) RuCl2L ] ( I - VI ) , L = 4 - cyanopyridine ( I ) , 2 - aminophenol ( II ) , 4 - aminophenol ( III ) , pyridazine ( IV ) , and [ ( eta6 - p - cymene ) RuClL2 ] PF6 ; L = cyanopyridine ( V ) , L = 2 - aminophenol(VI ) towards three cell lines was studied . - pubmed
The MIM2 - NMR method yields a mean absolute deviation ( MAD ) from unfragmented full molecule calculations of 0.01 ppm for 1H and 0.06 ppm for 13C chemical shifts . - pubmed
The 1H and 13C NMR chemical shifts calculated using our protocol give excellent agreement with experiments for two larger proteins , 2MC5 ( the helical part with 265 atoms ) and 3UMK ( 33 residue slice with 547 atoms ) . - pubmed
Overall , our target accuracy of ~0.3 ppm for 1H and ~2 - 3 ppm for 13C has been achieved for the larger proteins . - pubmed
The enzyme was eluted either at pH 6.8 in a concentration gradient of NADP+ , or at pH 8.5 in the presence of NADP+ in concentration gradients of either dicarboxylates or NaCl . - pubmed
2 '- AMP and its derivatives , 8 - bromoadenosine 2 '- phosphate and 8 -( l - aminohexyl ) aminoadenosine 2 '- phosphate , which were used in the synthesis of the adsorbent , all acted as enzyme inhibitors competitive with NADP+ . - pubmed
The chromatographic properties of the wild - type enzyme were compared with those of mutationally modified variants containing defined amino acid substitutions . - pubmed
The adsorbent showed a low capacity for the enzyme from mutant am1 ( Ser-336 replaced by Phe ) , a variant that has a localized defect in NADP binding , but an otherwise almost normal conformation , suggesting that non - specific interactions are at most weak . - pubmed
This is consistent with measurements in free solution that showed the am3 enzyme to have a higher Ki for 2 '- AMP than the wild - type enzyme . - pubmed
Methioninyl adenylate is a specific and potent inhibitor of the enzyme methionyl - tRNA synthetase and , consequently , of protein biosynthesis . - pubmed
The effect of NMMA requires functional MCP , cheA gene product , cheB gene product , and possibly cheX gene product . - pubmed
sudden dilution ) was detected by cheZ mutants with much higher sensitivity than that of a conventional repellent , indole , while both signals were rather poorly but equally detected by cheB mutants . - pubmed
The pulmonary lymphatics were shown to play an important role in the wash - out of the contrast media . - pubmed
Compared with that of the controls , the 24 - hour basal insulin secretion of islets cultured in the presence of cyproheptadine was significantly reduced from the 4th day of the study onwards . - pubmed
Labeling of CK8 / 18 was not inhibited by tunicamycin , suggesting that glycosylation was not N - linked . - pubmed
Acid hydrolysis of CK8 and CK18 , purified from [ 3H ] glucosamine - labeled cells , generated free glucosamine . - pubmed
In the presence of UDP -[ 3H ] galactose , galactosyltransferase catalyzed the labeling of cytokeratin 8 and 18 . - pubmed
beta - Elimination of the [ 3H ] galactose- labeled CK8/18 generated the disaccharide N - acetyllactosaminitol , indicating that cytokeratin 8 and 18 contain single O - linked N - acetylglucosamine residues . - pubmed
Using chemical analysis , the stoichiometry of glycosylation was found to be 1.5 and 2 molecules of N - acetylglucosamine / protein molecule of CK8 and CK18 , respectively . - pubmed
Peptide maps of [ 3H ] glucosamine - labeled CK8 / 18 showed that multiple peptides were labeled with the amino sugar . - pubmed
The biosynthetic and degradation rates of the carbohydrate moiety were faster than the protein core as determined by metabolic radiolabeling or pulse - chase experiments , respectively . - pubmed
Our results show that CK8 and 18 are glycosylated at multiple sites with a single O - linked N - acetylglucosamine . - pubmed
M3 - subtype muscarinic receptor that controls intracellular calcium release and inositol phosphate accumulation in gastric parietal cells . - pubmed
Other M2 - agonists , oxotremorine and arecoline , produced a partial [ Ca2 +]i increase , whereas M1 - agonists , pilocarpine and [ 4 - m - chlorophenylcarbamoyloxyl ]- 2 - butynyl - trimethylammonium , were without any significant effect . - pubmed
[ Ca2+]i rise was inhibited by selective muscarinic antagonists : atropine greater than 4 - diphenylacetoxy - N - methyl - piperidine methbromide greater than quinuclidinylbenzilate ( QNB ) greater than pirenzepine greater than 11 -[[ 2 -[( diethylamino ) methyl ]- 1 - piperidinyl ] acetyl ]- 5,11 - dihydro-6H - pyrido [ 2,3 - b ][ 1,4 ] benzodiazepine-6 - one , this sequence being characteristic of the involvement of an M3 - subtype . - pubmed
The IC50 values for inhibition of [ Ca2+]i increase by muscarinic antagonists were in good agreement with those obtained for inhibition of phospholipase C activation . - pubmed
Basal oxygen consumption fell from 16.1 + /- 0.72 ( + /- SEM ) to 10.1 + /- 0.47 ml / min per kg between 4 and 55 days of age , while breathing frequencies fell from 52.3 + /- 4.4 to 32.4 + /- 1.6 breaths / min over this period . - pubmed
Inhibition of neural phospholipase D activity by aminoglycoside antibiotics . - pubmed
These results indicate that aminoglycoside antibiotics inhibit phosphatidylcholine - PLD activity with comparable and sometimes greater potency than their well known inhibition of phosphoinositide - phospholipase C. - pubmed
The possibility that PLD inhibition could mediate some of the toxic side effects of aminoglycosides is suggested . - pubmed
The increase of activity can be inhibited by administering the selective dopamine D1 receptor agonist SKF 38393 , but not the selective D2 agonist quinpirole , or apomorphine . - pubmed
Conversely , in the dark , enzyme activity can be enhanced by administering the selective D1 antagonist SCH 23390 or haloperidol , but not the selective D2 antagonist ( -)- sulpiride . - pubmed
Kinetic analysis indicated that the apparent Vmax for the enzyme increases with little change in the apparent Km for the substrate 3,4 - dihydroxyphenylalanine or the cofactor pyridoxal-5 '- phosphate . - pubmed
We suggest that dopamine released in the dark tonically occupies D1 receptors and suppresses AAAD activity . - pubmed
The algorithm involved identification of ( a ) secondary peritonitis associated with gut perforation , based on previously proposed criteria in patients with neutrocytic ascites ( ascitic fluid total protein greater than 1 g / dl , glucose less than 50 mg / dl , and lactate dehydrogenase greater than the upper limit of normal for serum ) and ( b ) separation of spontaneous from secondary peritonitis ( unassociated with perforation ) based on the response of the ascitic fluid cell count to antibiotic therapy . - pubmed
Pentoxifylline has been shown to decrease endotoxin - induced tumor necrosis factor alpha production and reverse the inflammatory actions of interleukin-1 ( IL-1 ) and tumor necrosis factor on leukocyte function . - pubmed
Additionally , pentoxifylline attenuated meningeal inflammatory changes induced by intracisternal inoculation of 10 ng of rabbit recombinant IL-1 beta compared with results in either dexamethasone- or saline - treated animals . - pubmed
In addition , periodate treatment of the 24,25 -( OH ) 2 - D3 product resulted in complete loss of the product as measured by protein - binding assay . - pubmed
Activity of the 24 - hydroxylase in the crude solubilized extract was 6.3 pmol / h per mg , but was undetectable after partial purification by a p - chloroamphetamine - Sepharose column . - pubmed
However , further fractionation of this material by DEAE - Sepharose chromatography resulted in a further increase in 1 alpha - hydroxylase activity to 430 pmol / h per mg and detection of 24 - hydroxylase in a separate fraction at a level of 53 pmol / h per mg . - pubmed
Production of 1,25 -( OH ) 2 - D3 was linear with time up to 2 h and was dependent upon ferredoxin concentration as well as cytochrome P-450 concentration in the range of 0 - 40 nM. - pubmed
In the presence of excess ferredoxin and adequate amounts of cytochrome P-450 , 1,25 -( OH ) 2 - D3 production was also dependent upon substrate concentrations in the range of 0.25 to 2.5 microM yielding an estimated Km of 1 microM. - pubmed
This transcription appeared 2 to 4 h after addition of DMSO when expression of the c - myc gene was downregulated by blocking RNA elongation at the end of exon 1 . - pubmed
The formation of retinoyl - beta - glucuronide is catalyzed by a UDP - glucuronosyltransferase with apparent Km 's of 54.7 microM for all - trans - retinoic acid and 2.4 mM for UDP - glucuronic acid . - pubmed
The specific activity of the enzyme is increased by vitamin A deficiency . - pubmed
The adult lactase phenotype , lactose absorber or malabsorber , was determined using the lactose tolerance test with breath hydrogen assay in a group of Tuareg , a traditionally nomadic pastoralist population in the central Sahara . - pubmed
AQ - AH 208 produced a significant ( P less than 0.05 ) increase ( 35 % ) in transmural collateral perfusion whereas propranolol had no effect . - pubmed
Certain growth factors ( such as epidermal growth factor , platelet - derived growth factor , and transforming growth factors - alpha and - beta ) have been shown to stimulate the resorption of bone in organ culture by both prostaglandin - dependent and prostaglandin - independent pathways . - pubmed
The in vivo calcium - mobilizing activity of epidermal growth factor and transforming growth factor - alpha indicate that these or related growth factors need be considered as potential mediators of tumor - induced hypercalcemia . - pubmed
The reduced dexamethasone - suppressibility of plasma cortisol , which is characteristic of pregnancy , extends into the post - partum period . - pubmed
Antagonistic effects of apomorphine and haloperidol on rat striatal synaptosomal tyrosine hydroxylase . - pubmed
Stereospecificity of the oxidation of ethanol by catalase . - pubmed
[ Urinary phenolic acids during rest and muscular activity ] . - pubmed
We have investigated the mechanisms of blockade of calcium channel current by the dihydropyridines , e.g. - pubmed
Our results show that calcium channel blockade by dihydropyridine derivatives is strongly modulated by membrane potential . - pubmed
Our results suggest that dihydropyridines bind preferentially to the inactivated state of the calcium channel , and that the development of use - dependent block is related to the ionization constants of the compounds . - pubmed
This antisecretory action was blocked by naloxone and not mediated via an effect on tissue cyclic AMP level or adenylate cyclase activity . - pubmed
The first set of observations suggest that loperamide 's antisecretory effect is mediated via opiate receptors either distal to , or separate from the adenylate cyclase / cyclic AMP pathway . - pubmed
Release of sialic acid from substrates by sialidase in the presence of H2 [ 18O ] . - pubmed
All three kinases had molecular weights between 70 000 and 75 000 which increased to 170 000 - 200 000 in the presence of 2 mM ATP . - pubmed
Studies on the kinetic properties of the enzymes with thymidine and ATP as the substrates and dTTP as the inhibitor showed clear differences between TK 1 and TK 2 , but a close similarity between TK 2 and TK 2N. - pubmed
With thymidine as the variable substrate , TK 1 showed Michaelis - Menten kinetics , whereas TK 2 and TK 2N showed characteristic biphasic kinetics . - pubmed
With ATP as the variable substrate , all three enzymes showed positive cooperative kinetics , but TK 2 and TK 2N lost the cooperativity in the presence of dTTP . - pubmed
The results from inhibition studies showed , that dTTP was a cooperative inhibitor of TK 1 but a non - cooperative inhibitor of TK 2 and TK 2N. - pubmed
Ultrapure populations of G1- , S- , and G2 - M - phase cells were obtained through sequential centrifugal elutriation and flow cytometric cell sorting of Hoechst 33342 - stained cells . - pubmed
Angiotensin II ( ANG II ) and NaCl act on central receptors to cause pressor responses that may be mediated in part by the sympathetic nervous system . - pubmed
These findings illustrate that stimulation of sodium - sensitive and angiotensin - sensitive CNS receptors produces differential responses in sympathetic outflow . - pubmed
Inactivation of purified rat liver cytochrome P-450 by chloramphenicol . - pubmed
BACKGROUND & amp ; AIMS : Platelet - activating factor ( PAF ) is a potent inflammatory mediator implicated in the pathogenesis of inflammatory bowel disease and necrotizing enterocolitis . - pubmed
Metabolism by platelet - activating factor acetylhydrolase ( PAF - AH ) is the major pathway for platelet - activating factor degradation . - pubmed
Both intracellular and secreted PAF - AHs are calcium - independent and substrate - specific for phospholipids similar to PAF . - pubmed
Nuclear multicatalytic proteinase alpha subunit RRC3 : differential size , tyrosine phosphorylation , and susceptibility to antisense oligonucleotide treatment . - pubmed
NS RRC3 showed a significantly smaller M(r ) ( 24,000 ) than the cytoplasmic form ( M(r ) 28,000 ) , and only the nuclear form contained phosphotyrosine . - pubmed
In metabolic labeling experiments with [ 32P ] orthophosphate , the major nuclear and NS form observed showed an M(r ) of 24,000 , whereas no labeling of cytosolic RRC3 was observed . - pubmed
A minor 32P - labeled band of M(r ) 28,000 was also observed in nuclei , and this M(r ) 28,000 form was found in the soluble nuclear extract within MCP complexes . - pubmed
These results suggest that tyrosine phosphorylation of the cytosolic form ( M(r ) 28,000 ) rapidly triggers nuclear import , which is in turn quickly followed by conversion to the major M(r ) 24,000 form associated with NS . - pubmed
ECL cells store histamine and are considered a key element in the mechanisms of gastric acid secretion as controlled by gastrin stimulus . - pubmed
Kinetic analysis of expressed transport activity revealed a saturable transport system shared by amino beta - lactam antibiotics , dipeptides and selected angiotensin converting enzyme inhibitors . - pubmed
The present data indicate that PAMP as well as adrenomedullin is processed from an adrenomedullin precursor . - pubmed
The recommended dose for Phase II studies is 80 mg m-2 of CPT-11 and 60 mg m-2 of cisplatin . - pubmed
The charge density of renal interstitium was analysed from the volume of distribution of negative native albumin as compared with neutralized albumin , labelled with 125I and 131I , respectively . - pubmed
The results of kinetic studies of the enzyme activity indicated that ascorbic acid / Fe ( 2 +)-induced inhibition of the enzyme activity is mainly due to an increased Km value for the substrate . - pubmed
A decreased activity of the microsomal G-6 - Pase was also observed when the microsomes were incubated with aldehydes such as malondialdehyde , n - heptaldehyde , acetaldehyde , and trans-2 - nonenal . - pubmed
Glucose-6 - phosphate ( G-6 - P)effectively prevented ascorbic acid / Fe ( 2 +)- or trans-2 - nonenal - induced inhibition of the enzyme activity , but the substrate failed to protect the protein thiols in both systems . - pubmed
The results of fluorescence anisotropy measurements of diphenylhexatriene - labeled microsomes suggested that changes in the lipid dynamics are not directly related to peroxidation- mediated inhibition of the enzyme activity . - pubmed
Based on these results , a possible reason for the inhibition of the microsomal G-6 - Pase activity associated with ascorbic acid / Fe ( 2 + ) treatment is discussed . - pubmed
In T cells , overexpression of wild - type SLP-76 results in a hyperactive receptor , while expression of a SLP-76 molecule that is unable to be tyrosine - phosphorylated attenuates receptor function . - pubmed
In addition , the SH2 domain of SLP-76 is required for T - cell receptor function , although its role is independent of the ability of SLP-76 to undergo tyrosine phosphorylation . - pubmed
As SLP-76 interacts with both Grb2 and phospholipase C - gamma1 , these data indicate that phosphorylation of SLP-76 by ZAP-70 provides an important functional link between the T - cell receptor and activation of ras and calcium pathways . - pubmed
The heart failed to generate contractile force and increased the resting tension markedly within 5 min of perfusion with xanthine plus xanthine oxidase . - pubmed
The protective effects of vanadate on the loss of developed tension and increased resting tension due to xanthine plus xanthine oxidase were dose - dependent ( 0.1 - 5 microM ) . - pubmed
The sarcolemmal Ca ( 2 +)- pump ( ATP - dependent Ca2 + uptake and Ca ( 2 +)- stimulated ATPase ) and Na(+)-dependent Ca2 + uptake activities were decreased upon perfusing the hearts with a medium containing xanthine plus xanthine oxidase for 5 min ; these effects were prevented by the addition of 2 - 4 microM vanadate in the perfusion medium . - pubmed
The signals for superoxide radicals produced by xanthine plus xanthine oxidase , as detected by electron paramagnetic resonance spectroscopic technique , were inhibited by 5 - 100 microM vanadate . - pubmed
This reduced conductance was reflected in a 35 % decrease in binding of radiolabeled saxitoxin , a sodium channel - specific ligand , indicating expression of fewer channels in these neurons . - pubmed
Thus , the altered regulation of voltage - gated sodium channels in Ts16 neurons is apparently a post - transcriptional event and possible mechanisms are discussed . - pubmed
In the present study , we have examined , in vitro , the direct effects of vinblastine ( VBL ) , 5 - fluorouracil ( 5 - FU ) , melphalan ( L - PAM ) and the novel tubulin inhibitor combretastatin A-1 ( CBS ) on endothelial permeability under normal and tumour simulated conditions . - pubmed
The changes induced by VBL and CBS were accompanied by contraction of the endothelial F - actin cytoskeleton . - pubmed
Both VBL and CBS have vascular components in their mode of action which may lead to vascular collapse and tumour necrosis . - pubmed
In addition to a TNF - alpha - mediated pathway , there is growing evidence that some particles such as quartz and crocidolite can directly activate lung epithelial cells to release chemokines such as macrophage inflammatory protein-2 , cytokine - induced neutrophil chemoattractant , and interleukin-8 . - pubmed
Six mutants , called LT7 K ( Arg to Lys ) , LT61F ( Ser to Phe ) , LT112 K ( Glu to Lys ) , LT118E ( Gly to Glu ) , LT146E ( Arg to Glu ) and LT192 G ( Arg to Gly ) were constructed by the replacement of one amino acid at one position of the A1 subunit to another using site - directed mutagenesis . - pubmed
Importantly , the rBAT 1.1 cDNA was derived from a 7.5 - kb messenger RNA ( mRNA ) and did not encode a functional selenoenzyne unless an enabling selenocysteine insertion sequence was appended to the presumed coding region and this cDNA . - pubmed
Direct analysis of the translation products programmed by the native SeD2 mRNA in cAMP - stimulated astrocytes was performed using antisense deoxynucleotides and hybrid selection strategies . - pubmed
However , no immunoreactive 30 - kDa SeD2 protein was identified by Western analysis , immunoprecipitation , or immunocytochemistry , and the specific C - terminus antiserum failed to immunoprecipitate deiodinase activity from ( Bu ) 2cAMP - stimulated astrocytes , brown adipose tissue or brain . - pubmed
Instead , the native 7.5 - kb SeD2 mRNA encoded a 15 - kDa protein that terminated at the first UGA codon and contained the catalytically inactive , N - terminal 129 amino acids of SeD2 . - pubmed
On the other hand , testing the tyrosine phosphorylation of FAK , revealed significant increases in diabetes . - pubmed
This demonstrated that the amounts of FAK co - precipitated with beta1 , as well as its tyrosine - phosphorylation , are in fact reduced in diabetic conditions . - pubmed
The bcl-2 transgene was strongly expressed in these cells , and resulted in protection of neuronal cultures from death triggered by serum deprivation or exposure to staurosporine . - pubmed
Although specific DNA lesions that involve base loss or modification stimulate nucleic acid cleavage mediated by type II topoisomerases , the effects of deoxyribose sugar ring modification on enzyme activity have not been examined . - pubmed
Therefore , the effects of incorporated araC residues on the DNA cleavage / religation equilibrium of human topoisomerase IIalpha and beta were characterized . - pubmed
These findings broaden the range of DNA lesions known to alter topoisomerase II function and raise the possibility that this enzyme may mediate some of the cellular effects of araC. - pubmed
Gastric antral G - cells process progastrin at Arg(94)Arg(95 ) and Lys(74)Lys(75 ) residues generating gastrin heptadecapeptide ( G17 - NH(2 ) ) . - pubmed
Conversely , duodenal G - cells process progastrin to gastrin tetratriacontapeptide ( G34 - NH(2 ) ) with little processing at Lys(74)Lys(75 ) . - pubmed
Although mutation of the Lys(74)Lys(75 ) site within G34 - NH(2 ) to Lys(74)Arg(75 ) resulted in the production of primarily G17 - NH ( 2 ) rather than G34 - NH ( 2 ) , inhibition of PC1 / PC3 did not significantly inhibit processing at the Lys(74)Arg(75 ) site . - pubmed
Endothelin-1 ( ET-1 ) is a 21 amino acid peptide that binds to G - protein - coupled receptors to evoke biological responses . - pubmed
ET-1 , but not angiotensin - II ( A - II ) , stimulated glucose uptake in a dose - dependent manner with an EC50 value at approximately 1 nM , and an approximately 2 - fold stimulation at 100 nM. - pubmed
As a comparison , insulin stimulated glucose uptake in a dose - dependent manner with an EC50 value at 1 nM , and a 2.5 - fold stimulation at 100 nM. - pubmed
The effect of ET-1 on glucose uptake was blocked by A-127722 , an antagonist selective for the ET ( A )- receptor . - pubmed
The effect of ET-1 on glucose uptake was not inhibited by inhibitors for protein kinase C ( PKC ) , protein kinase A ( PKA ) and phosphatidylinositol-3 - kinase ( PI3 '- kinase ) . - pubmed
Aquaporin-2 ( AQP-2 ) is the vasopressin - regulated water channel expressed in the apical membrane of principal cells in the collecting duct and is involved in the urinary concentrating mechanism . - pubmed
In addition , these results support the idea that AQP-2 , and probably other aquaporins , are involved in water absorption in the colon . - pubmed
The plasma lactate , A and NA were analysed at rest ( La0 , A0 and NA0 ) , immediately at the end of the exercise ( Amax and NA(max ) ) and after 5 min recovery ( La(max ) , A5 and NA5 ) . - pubmed
Cell surface biotinylated MPR and ( 125 ) I - labeled transferrin are accumulated in cellugyrin - positive vesicles and to a lesser extent in cellugyrin - negative vesicles . - pubmed
An average cellugyrin - positive vesicle carries not more than one molecule of either Glut4 , insulin - responsive aminopeptidase ( IRAP ) , or transferrin receptor ( TfR ) , whereas cellugyrin - negative vesicles contain five to six molecules of Glut4 , more than 10 molecules of IRAP , and still one molecule of TfR per vesicle . - pubmed
Thus hepatic glucose production is depressed after LPS administration , which is , at least in part , due to the downregulation of phosphoenolpyruvate carboxykinase ( PEPCK ) expression . - pubmed
Nucleotide competition assays show the kinase to be ATP specific . - pubmed
Cystathionine - beta - synthase ( CBS ) catalyzes the condensation of serine and homocysteine to form cystathionine , an intermediate step in the synthesis of cysteine . - pubmed
The lipopolysaccharide - induced production of IL-1 , TNF and NO by peritoneal macrophages was significantly reduced . - pubmed
Furthermore , RSG significantly recovered the picryl chloride - induced delayed - type hypersensitivity to almost normal levels from the higher or lower levels induced by different treatments of cyclophosphamide with a normalization of CD4 / CD8 ratio . - pubmed
This study was designed to compare measurements of cardiac output as produced by the partial CO ( 2 ) rebreathing ( NICO ) , bolus ( BCO ) , and continuous thermodilution ( CCO ) methods of monitoring cardiac output . - pubmed
[ Phosphotransferase action of phosphomonoesterase in hepatic metabolism of nucleosides ] . - pubmed
Biological concentration by killer clams of cobalt-60 from radioactive fallout . - pubmed
The activity of the mutant p53 in tumor cells is restorable based on the fact that PAb241 antibody against the carboxy - terminus of p53 and peptides corresponding to the p53 carboxy - terminus can restore specific DNA - binding ability to some mutant p53 proteins . - pubmed
High throughout screening of chemical libraries has led to the identification of a group of small synthetic molecules such as CP-31398 , which can restore p53 function to mutant p53 by stabilizing the active conformation of the protein that is destabilized in many mutants . - pubmed
Further understanding of the mechanisms by which CP-31398 and PRIMA-1 restore p53 activity may not only lead to discovery of more potent analogs but may also suggest new strategies for p53 - targeting in tumor therapy . - pubmed
OBJECTIVE : 11 beta - hydroxysteroid dehydrogenase type 1 ( 11 beta HSD1 ) converts inactive cortisone to active cortisol . - pubmed
RESULTS : Prior to GH , the ratio Fm / Em was greater with hydrocortisone compared with cortisone acetate replacement ( 1.17 + /- 0.28 and 0.52 + /- 0.09 respectively , P & lt ; 0.001 ) or with normal subjects ( normal males : 0.81 + /- 0.24 , females 0.66 + /- 0.14 ) . - pubmed
Conversely , the ratio of urinary free cortisol / cortisone did not change indicating unchanged 11 beta HSD2 activity . - pubmed
Pioglitazone reduced the expression of TIMP-1 and TIMP-2 mRNA without changing MMP-13 mRNA expression compared to the liver fed a CDAA diet alone . - pubmed
In vitro , pioglitazone prevented the activation of hepatic stellate cells resulting in reducing the expression of type I procollagen , MMP-2 , TIMP-1 , and TIMP-2 mRNA with increased MMP-13 mRNA expression . - pubmed
Glutamate racemase ( MurI ) catalyzes the racemization of glutamate ; two cysteine residues serve as catalytic acid and base . - pubmed
In what we term substrate flipping , Glu suddenly rotates into a binding pattern that resembles the post - racemization state of the other enantiomer , i.e. - pubmed
Peroxidative aggregation of alpha - synuclein requires tyrosines . - pubmed
Although it is known that peroxidative chemistry leads to the aggregation of alpha - synuclein in vitro , the specific amino acid types of alpha - synuclein involved in this type of aggregation have not been identified . - pubmed
ABCA1 has been established to be required for the efflux of cholesterol and phospholipids to apolipoproteins such as apoA - I. - pubmed
At present , it is unclear whether ABCA1 - mediated lipid exposure is specific with regard to lipid headgroups and whether it requires calcium activation and the presence of a lipid acceptor . - pubmed
By using C6 - N -( 7 - nitrobenz-2 - oxa-1,3 - diazol-4 - yl ) ( NBD)-labeled phospholipid analogues , we observed elevated redistribution of phosphatidylserine and phosphatidylethanolamine but not of phosphatidylcholine analogues from the cytoplasmic to the exoplasmic leaflet of the plasma membrane of ABCA1 - GFP expressing cells . - pubmed
Whereas glyburide affected neither the level of exofacial endogenous PS nor the outward movement of the amino phospholipid analogues , the latter was sensitive to intracellular Ca2 + in ABCA1 - GFP expressing cells , further enhancing outward analogue redistribution with respect to control cells . - pubmed
Glyburide raised the level of receptor - mediated endocytosis in the ABCA1 - GFP expressing cell to the level of control cells in the absence of glyburide . - pubmed
We report that TA ( L ) activity participates in the control of PAF levels in monocytes and macrophages and that its regulation differs in these two types of cells . - pubmed
When 12 PBH was chosen as the time point for in vitro intervention with the application of specific NF - kappaB inhibitor pyrrolidine dithiocarbamate ( PDTC ) , PKC inhibitor H-7 and PNS , both TNF - alpha mRNA expression and NF - kappaB activity decreased significantly . - pubmed
This child is alive and well today because of these well - trained camp counselors . - pubmed
Circulating total testosterone and insulin - like growth factor - I ( IGF - I ) experienced significant ( P & lt ; 0.05 ) declines , and cortisol was significantly increased . - pubmed
IGF - I and cortisol , but not total testosterone , were correlated with losses of tissue mass . - pubmed
With severe weight loss ( & gt;or=13 % of body mass ) , IGF - I and cortisol correlate more closely with soft - tissue tissue adaptations than does testosterone . - pubmed
Serum 25 - OH - vitamin D was 18.2ng / ml ( normal , 20 - 40ng / ml ) and serum parathyroid hormone was 620pg / ml ( normal , 15 - 65pg / ml ) , suggesting secondary hyperparathyroidism . - pubmed
Although the physiological role of CP is uncertain , its role in iron ( Fe ) homeostasis and protection against free radical - initiated cell injury has been widely documented . - pubmed
Previous studies showed the existence of two molecular isoforms of CP : secreted CP ( sCP ) and a membrane glycosylphosphatidylinositol ( GPI)-anchored form of CP ( GPI - CP ) . - pubmed
Also , expression of CP in huPBL is demonstrated by immunofluorescence with confocal microscopy and flow cytometry analysis using cells isolated from healthy blood donors with normal Fe status . - pubmed
In this context , the involvement of lymphocyte - derived CP on host defense processes via its anti / prooxidant properties is proposed , giving further support for a close functional interaction between the immune system and the Fe metabolism . - pubmed
Hydrodynamic characterization of the SufBC and SufCD complexes and their interaction with fluorescent adenosine nucleotides . - pubmed
Genetic experiments in some organisms found that the ATPase SufC is essential , though its role in Fe - S biogenesis remains unclear . - pubmed
Both SufBC and SufCD bound mantATP and mantADP much more tightly than does SufC alone . - pubmed
Compared with heparin plus GPI , bivalirudin monotherapy resulted in similar protection from ischemic events with less major bleeding . - pubmed
Compared with treatment with heparin plus GPI , bivalirudin monotherapy provides similar protection from ischemic events with less major bleeding , resulting in a significant reduction in net adverse clinical outcomes . - pubmed
Mutation on Ikappa kinase beta cysteine 179 markedly diminishes the effect of A(4)-NP , suggesting that A(4)-NP acts via thiol modification at this residue . - pubmed
Accordingly , the effects of A(4)-NP are independent of peroxisome proliferator - activated receptor - gamma and are dependent on an intact reactive cyclopentenone ring . - pubmed
Thus , we have demonstrated that A(4)/J(4)-NPs , formed via the oxidation of DHA , are potent inhibitors of NF - kappaB signaling and may contribute to the anti - inflammatory actions of DHA . - pubmed
The results obtained were as follows : an extraction yield from 2.64 to 4.69 g/100 g ; an AGA content between 49.8 and 64.06 g/100 g ; a content of metoxil between 4.72 and 7.18 g/100 g ; a degree of esterification between 37.94 and 52.20 % ; an equivalent weight from 385.47 to 464.61 g / equivalent of H+ , and a degree of gelation between 28.64 and 806.03 g force . - pubmed
Activities of tryptophan decar - boxylate ( TDC ) and anthranilate synthase ( AS ) , two enzymes In the pathway leading to ajmalicine , were higher after induction with 40 g / L glucose than after induction with 60 or 80 g / L glucose . - pubmed
Experiments with different media containing mixtures of glucose and the nonpermeating osmotic agent xylose , and using an already induced culture as inoculum , revealed that a minimum amount of glucose is required to support ajmalicine production after enzyme induction . - pubmed
Differences in enzyme induction and ajmalicine production at different glucose levels could not be explained by the intracellular concentrations of glucose , fructose , sucrose , or starch . - pubmed
Older antihypertensive drugs ( diuretics , beta - blockers , centrally acting ) exert negative results , whereas newer drugs have either neutral ( calcium antagonists , angiotensin - converting enzyme inhibitors ) or beneficial effects ( angiotensin receptor blockers ) . - pubmed
Most of the calcium that activates cardiac contraction comes from the sarcoplasmic reticulum ( SR ) from where it is released through the RyR. - pubmed
The amplitude of the systolic Ca transient depends steeply on the SR Ca content and it is therefore important that SR content be regulated . - pubmed
This regulation occurs via changes of SR Ca content affecting systolic Ca and thence sarcolemmal Ca fluxes . - pubmed
The main part of the review considers the effects of modulating the RyR on systolic Ca . - pubmed
Potentiation of RyR opening produces an increase of the amplitude of the Ca transient but this effect disappears within a few beats because the increased sarcolemmal efflux of Ca decreases SR Ca content . - pubmed
Some cardiac arrhythmias result from release of Ca from the SR in the form of waves . - pubmed
This is best known to occur when the SR is overloaded with calcium . - pubmed
Mutations in the RyR also produce cardiac arrhythmias attributed to Ca waves due to leaky RyRs and a similar leak has been suggested to contribute to arrhythmias in heart failure . - pubmed
We show that , due to compensatory changes of SR Ca content , simply making the RyR leaky does not produce Ca waves in the steady state and that SR Ca content is critical in determining whether Ca waves occur . - pubmed
AIM : The aim of this study was to determine if topical atropine , used to retard axial length elongation and myopia progression , had any effect on ocular astigmatism . - pubmed
AR mutants bind promiscuous steroids , and may convert AR antagonists to agonists . - pubmed
Various non - hormonal growth factor receptors transactivate the AR , even in absence of androgens ( outlaw pathway ) . - pubmed
Degenerating somas ( neuronal death ) and axonal / synaptic endings ( terminal degeneration ) were depicted by a silver technique , and functionally affected cortical neuronal subpopulations by Egr-1 , c - Fos , and FosB / DeltaFosB - immunolabeling . - pubmed
In intact males , MK801 triggered a c - Fos induction that remained high for more than 24 h in selected layers of the retrosplenial , somatosensory and entorhinal cortices . - pubmed
MK801 - induced neurodegeneration reached its peak at 72 h. Degenerating somas were restricted to layer IV of the granular subdivision of the retrosplenial cortex , and were accompanied by suppression of Egr-1 immunolabeling . - pubmed
Induction of FosB / DeltaFosB by MK801 also extended to the same cortical layers affected by terminal degeneration , likely reflecting the damage of synaptic connectivity . - pubmed
Degenerative somas in layer IV of the retrosplenial cortex significantly increased , with a parallel enhancement of terminal degeneration and FosB / DeltaFosB - expression in the mentioned cortical structures , but no additional areas were affected . - pubmed
This article aims to focus on the effect of H ( 2 ) O ( 2 )-redox homeostasis on hair follicle pigmentation via tyrosinase , its substrate supply and signal transduction as well as the role of methionine sulfoxide repair via methionine sulfoxide reductases A and B ( MSRA and B ) . - pubmed
PPBD was mapped to the highly conserved 54 amino acids at the C - terminal end of tubby and Tulp1 . - pubmed
In addition , exercise disrupted the clustering of cytokine expression and improved glucose tolerance , without reducing body fat or cytokine levels . - pubmed
Exercise improves glucose tolerance and disrupts the coexpression of proinflammatory cytokines , suggesting that increased aerobic exercise may act independently of weight loss in promoting joint health . - pubmed
RESULTS : Le antigen phenotyping revealed a significant ( p & lt ; .0001 ) association between type 1 ( Le ( a ) and Le ( b ) ) expression and strains of East Asian origin . - pubmed
Tyrosine ammonia lyase catalyzes the deamination of L : - tyrosine to trans - coumaric acid . - pubmed
The disruption of the bagA gene abolished trans - coumaric acid and bagremycins production . - pubmed
Thus , trans - coumaric acid is a precursor for biosynthesis of bagremycins and the bagA gene codes for tyrosine ammonia lyase to synthesize trans - coumaric acid . - pubmed
A hypothalamic neuropeptide , gonadotropin - releasing hormone ( GnRH ) , is the primary factor regulating gonadotropin secretion . - pubmed
An inhibitory hypothalamic neuropeptide for gonadotropin secretion was , until recently , unknown , although gonadal sex steroids and inhibin can modulate gonadotropin secretion . - pubmed
GnIH acts on the pituitary and on GnRH neurons in the hypothalamus via a novel G protein - coupled receptor ( GPR147 ) . - pubmed
GnIH decreases gonadotropin synthesis and release , inhibiting gonadal development and maintenance . - pubmed
More recent evidence suggests that GnIH also acts both upstream of the GnRH system and at the level of the gonads to appropriately regulate reproductive activity across the seasons and during times of stress . - pubmed
Tumor cell repopulation was quantified by bromodeoxyuridine incorporation and Ki67 by immunohistochemistry and/or flow cytometry . - pubmed
In vitro cell killing was determined by classic chromium - released assay , and reverse transcription PCR ( RT - PCR ) was carried out to determine the gene expression of associated cytokines . - pubmed
Tumor cell repopulation during the intervals of cisplatin was inhibited by CTLA-4 blockade . - pubmed
When comparing roots of fully sulfate - starved plants with sulfate - starved split - root roots , expression of several potentially OAS responsive genes was attenuated in split - roots depending on the shoot sulfate status and the local root O - acetylserine concentration . - pubmed
In contrast , high - affinity sulfate transporters displayed similar expression in sulphate - starved split - roots and the corresponding controls . - pubmed
Sucrose preference was not observed in TS group . - pubmed
Moreover , patients with constitutive IFN - stimulated gene ( ISG ) expression have a poor response to treatment with pegylated IFN - alpha ( PegIFN - alpha ) and ribavirin . - pubmed
HLA - DP and IL28B polymorphisms : influence of host genome on hepatitis B surface antigen seroclearance in chronic hepatitis B. - pubmed
BACKGROUND : The roles of single - nucleotide polymorphisms ( SNPs ) at HLA - DP and IL28B loci on hepatitis B surface antigen ( HBsAg ) seroclearance in chronic hepatitis B ( CHB ) infection are unknown . - pubmed
METHODS : We compared the HLA - DP ( rs3077 , rs9277378 , rs3128917 ) and IL28B ( rs12979860 , rs8099917 ) polymorphisms of 203 CHB patients achieving spontaneous HBsAg seroclearance with 203 age- and sex - matched CHB patients without HBsAg seroclearance ( controls ) . - pubmed
HLA - DP rs3077 was associated with HBsAg seroclearance in terms of allelic frequency ( minor allele A vs major allele G , P = .035 ; odds ratio [ OR ] , 0.699 ; 95 % confidence interval [ CI ] , .501-.976 ) and genotypic frequency ( AA vs GG / GA , P = .014 ; OR , 0.295 ; 95 % CI , .106-.822 ) . - pubmed
Haplotype analysis of HLA - DP polymorphisms showed haplotype block GAT ( rs3077 / rs9277378 / rs3128917 ) to be associated with HBsAg seroclearance ( OR , 2.17 ; 95 % CI , 1.06 - 4.45 , P = .034 ) . - pubmed
Influence of HLA - DP polymorphisms on HBsAg seroclearance was more pronounced in younger patients , with the OR for rs3077 minor allele A and haplotype block GAT being 0.560 and 2.68 , respectively , among patients aged & lt;50 years ( P = .027 and P = .047 , respectively ) . - pubmed
IL28B haplotype block CG ( rs12979860 / rs8099917 ) was associated with HBsAg seroclearance ( OR , 10.5 , P = .026 ) . - pubmed
CONCLUSIONS : Specific SNPs in HLA - DP and IL28B locus , through individual and haplotype analysis , were associated with a higher chance of HBsAg seroclearance in CHB infection . - pubmed
METHODS AND RESULTS : We conducted metabolite analyses , qRT - PCR of the glycerol reductive pathway [ glycerol dehydratase ( DhaB ) and 1,3 - PDO dehydrogenase ( DhaT ) ] and DhaT activity assays at different pH , temperature and initial glycerol concentrations . - pubmed
The decreased DhaT activity eventually caused the shutdown of 1,3 - PDO production . - pubmed
In this review , we highlighted the recent progress in multinuclear platinum complexes and ruthenium complexes as anticancer agents , and their novel DNA binding properties such as phosphate clamps , long range DNA cross links , bisintercalation , interduplex cross links and DNA - protein cross - links were summarized to shed light on the rational design of polynuclear complexes as anticancer agents . - pubmed
Here we investigated the effect of tanshinone II A on Tat - induced HIV-1 transactivation and the redox signaling pathway involved in it . - pubmed
Tanshinone II A reversed Tat - induced inhibition of SIRT1 activity but not SIRT1 protein expression . - pubmed
Tanshinone II A - evoked Nampt expression was mediated by AMPK signaling pathway . - pubmed
PURPOSE : To evaluate the topical instillation of K-115 , a selective Rho - associated coiled coil - containing protein kinase ( ROCK ) inhibitor , on intraocular pressure ( IOP ) , ocular distribution , and aqueous humor dynamics in experimental animals . - pubmed
METHODS : Kinase inhibition by K-115 was measured by biochemical assay . - pubmed
RESULTS : Biochemical assay showed that K-115 had selective and potent inhibitory effects on ROCKs . - pubmed
CONCLUSIONS : These results indicated that K-115 ophthalmic solution , a selective and potent ROCK inhibitor , is a novel and potent antiglaucoma agent . - pubmed
Isopentenyl diphosphate isomerase ( IDI ) is a key enzyme in the isoprenoid biosynthetic pathway and is required for all organisms that synthesize isoprenoid metabolites from mevalonate . - pubmed
Steady - state kinetic studies of the enzyme indicated that FMNH2 ( KM = 0.3 muM ) bound before isopentenyl diphosphate ( KM = 40 muM ) in an ordered binding mechanism . - pubmed
At 30 days , TRA compared to TFA was associated with a significant reduction of cardiovascular mortality ( 5.2 % vs 10.5 % ; P<.001 ) , significant MACE reduction ( 7.3 % vs 12.5 % ; P<.001 ) , fewer access - site complications ( 0.9 % vs 8.2 % ; P<.001 ) , and lower TIMI major bleeding ( 1.1 % vs 4.3 % ; P<.001 ) . - pubmed
We report here a facile approach to fabricating poly ( l - lactic acid )-grafted hydroxyapatite ( g - HAp)/poly ( lactide - co - glycolide ) ( PLGA ) nanocomposite ( NC ) porous scaffolds by solvent evaporation of Pickering high internal phase emulsion ( HIPE ) templates , where g - HAp nanoparticles act as particulate stabilizers . - pubmed
Enzymatic Studies of Isoflavonoids as Selective and Potent Inhibitors of Human Leukocyte 5 - Lipo - Oxygenase . - pubmed
Continuing our search to find more potent and selective 5 - LOX inhibitors , we present now the enzymatic evaluation of seventeen isoflavones ( IR ) and nine isoflavans ( HIR ) , and their in vitro and in cellulo potency against human leukocyte 5 - LOX . - pubmed
Of the 26 compounds tested , 10 isoflavones and 9 isoflavans possessed micromolar potency , but only three were selective against 5 - LOX ( IR-2 , HIR-303 , and HIR-309 ) , with IC50 values at least 10 times lower than those of 12 - LOX , 15 - LOX-1 , and 15 - LOX-2 . - pubmed
Of these three , IR-2 ( 6,7 - dihydroxy-4 - methoxy - isoflavone , known as texasin ) was the most selective 5 - LOX inhibitor , with over 80 - fold potency difference compared with other isozymes ; Steered Molecular Dynamics ( SMD ) studies supported these findings . - pubmed
The presence of the catechol group on ring A ( 6,7 - dihydroxy versus 7,8 - dihydroxy ) correlated with their biological activity , but the reduction of ring C , converting the isoflavones to isoflavans , and the substituent positions on ring B did not affect their potency against 5 - LOX . - pubmed
Cognitive functioning , leisure - time physical activity ( LTPA ) , serum trace elements - Fe , Cu , Zn , Zn / Cu , and GADAs were assessed using LOTCA battery , pre - validated physical activity ( PA ) questionnaire , atomic absorption , and immunoassay techniques , respectively . - pubmed
LOTCA - cognitive scores correlated positively with sex , HbA(1c ) , Fe , Cu , Zn , and Zn / Cu ratio , and negatively with age , PA , body mass index , and anti - GADAs . - pubmed
In this study , we used affinity purification coupled with liquid chromatography and mass spectrometric analysis of the digested protein for consistent and simultaneous detection of the full amino acid sequence of a therapeutic IgG4 monoclonal antibody , MAB1 . - pubmed
Macronutrient meal ingestion significantly reduced FMD , an effect that was moderated by body mass index , sex and two - way interactions between disease status and both baseline FMD and baseline blood glucose levels . - pubmed
Using protease cleavage - predicting software and site - directed mutagenesis , we identified that calpain-1 cleaves hERG at position Gly-603 in the S5 - pore linker of hERG . - pubmed
The aim of this study was to evaluate the consumption , performance , feed efficiency , glucose concentration , and the cost per kilogram of weight gain of crossbred Holstein - Gyr heifers ( n = 33 ) subjected to one of the three waste milk step - down strategies : 6L-30d/4L-60d/2L-90d ( T1 ) , 6L-45d/4L-60d/2L-90d ( T2 ) , and 8L-30d/6L-60d/3L-90d ( T3 ) . - pubmed
Insufficient improvement on oral antihyperglycemic therapy and poor feasibility of daily subcutaneous insulin therapy led to an investigation into an alternative therapy with extended - release exenatide , a glucagon - like peptide-1 ( GLP-1 ) mimetic , at a dosage of 0.13 mg / kg subcutaneously once per month . - pubmed
This defect is in part due to increased apoptosis of HSPCs with reduced Erg dosage , a phenotype that becomes more drastic during 5 - FU - induced stress hematopoiesis . - pubmed
, psychoactive drugs such as LSD and psilocybin that exert their effects primarily through agonist activity at serotonin 2A ( 5HT2A ) receptors . - pubmed
Cardiopulmonary bypass ( CPB ) surgery , despite heparin administration , elicits activation of coagulation system resulting in coagulopathy . - pubmed
Intracellular Calcium Mobilization Is Required for Sonic Hedgehog Signaling . - pubmed
Reduced expression or modified activity of ryanodine receptor ( RyR ) intracellular calcium release channels shifted the allocation of Shh - dependent cell fates in the somitic muscle and neural tube . - pubmed
Mosaic analysis revealed that RyR - mediated calcium mobilization is required specifically in Shh ligand - receiving cells . - pubmed
As modulation of RyR activity modifies tissue patterning , we hypothesize that alterations in intracellular calcium mobilization contribute to both birth defects and evolutionary modifications of morphology . - pubmed
We found a positive correlation between sucrose preference and osteoprotegerin / receptor activator of nuclear factor kappaB ligand ratio . - pubmed
The phosphorylation of MAP2c , stathmin , and JNK1 in the male pup hippocampus was significantly decreased in DEHP treatment groups compared to controls . - pubmed
Transmission electron microscopy ( TEM ) analysis demonstrated that the ZIF-8 nanocrystals were gradually etched out in the aqueous media within 48 h , resulting in hollow nanocapsules . - pubmed
Study data and outcome measures ( fasting blood glucose , glycated haemoglobin and serum insulin ) were extracted from included studies . - pubmed
Vitamin D supplementation was associated with a decrease in fasting blood glucose by a mean of 0.46 mmol / L ( -0.68 , -0.25 ) ( p & lt ; 0.001 ) , glycated haemoglobin by a mean of 0.37 % ( -0.65 , -0.08 ) ( p & lt ; 0.01 ) and serum insulin concentration by mean of 4.10 microIU / mL ( -5.50 , -2.71 ) ( p & lt ; 0.001 ) compared to controls . - pubmed
This study aimed to analyze the effect of natural antioxidants , 6.5 % proanthocyanidin ( PA ) and 25 % bamboo salt ( BS ) on the reversal of NaOCl - induced reduced bond strength of an epoxy resin sealer to dentin . - pubmed
Both 6.5 % PA and 25 % BS were capable of reversing the compromised PBS of AH Plus to NaOCl - treated dentin . - pubmed
Conclusion : Final irrigation with antioxidants such as PA and BS eliminates the risk of reduced bond strength of AH Plus to root canal walls , which ensues following the use of NaOCl as an irrigant . - pubmed
In contrast , prior studies reported that plasmalemmal NMDA GluN1 increased in ERbeta - expressing PVN dendrites in males and early , but not late stage AOF females . - pubmed
Its unique catalytic mechanism involving the two - electron reduction of quinone - based compounds has made it a useful target to exploit in the design of hNQO1 fluorescent chemosensors and hNQO1 - activatable - prodrugs . - pubmed
Native phenalenone is liberated in the presence of hNQO1 resulting in the production of cytotoxic singlet oxygen upon irradiation . - pubmed
Plant K + uptake typically consists low - affinity mechanisms mediated by Shaker K + channels ( AKT / KAT / KC ) and high - affinity mechanisms regulated by HAK / KUP / KT transporters , which are extensively studied . - pubmed
HvAKT2 and HvHAK1 are both localized to the plasma membrane and have high selectivity to K+ and Rb + over other tested cations . - pubmed
Overexpression of HvAKT2 and HvHAK1 enhanced K + uptake and H + homeostasis leading to drought tolerance in these transgenic lines . - pubmed
Moreover , HvAKT2- and HvHAK1 - overexpressing lines showed distinct response of K + , H + , and Ca2 + fluxes across plasma membrane and production of nitric oxide and hydrogen peroxide in leaves as compared to the wild type and silenced lines . - pubmed
Upon tissue disruption , glucosinolates come into contact with myrosinase , which initiates their breakdown to biologically active compounds . - pubmed
Among these , the formation of epithionitriles is triggered by the presence of epithiospecifier protein ( ESP ) and a terminal double bond in the glucosinolate side chain . - pubmed
Biochemical characterization of heterologous expressed BoESP isoforms revealed different substrate specificities towards seven glucosinolates : all isoforms showed epithiospecifier activity on alkenyl glucosinolates , but not on non - alkenyl glucosinolates . - pubmed
oleracea ESPs in glucosinolate breakdown . - pubmed
In conclusion , the most important bioactive components - anthraquinone , kaempferol , and vanillic acid - can alleviate depression symptoms by regulating MAOA , MAOB , and ESR1 . - pubmed
A novel highly frequent single - nucleotide polymorphism site of cadherin 23 in clear cell renal cell carcinoma with sarcomatoid differentiation based on whole exome sequencing . - pubmed
A highly frequent mutation of CDH23 ( rs3802711 ) was observed in CCRCCS that resulted in an alteration in the highly conserved calcium - binding site in the three - dimensional ( 3D ) structure mediating the functions of cadherins . - pubmed
Inhibition of semaphorin 4D enhances chemosensitivity by increasing 5 - fluorouracile - induced apoptosis in colorectal cancer cells . - pubmed
In this study , we explored the relationship between silencing SEMA4D expression and 5 - fluorouracil ( 5 - FU ) response in the colorectal cancer cell line . - pubmed
Our data demonstrate that SEMA4D silencing results in strikingly elevated apoptosis in response to 5 - FU treatment and leads to down - regulation of Bcl-2 and overexpression of Bax , P53 , and caspase-3 in protein levels . - pubmed
However , mRNA expression of E - cadherin as an epithelial marker was significantly increased in 5 - FU treatment combined with siRNA SEMA4D. - pubmed
We demonstrate that infection of wt macrophages results in activation of Asm , which increases reactive oxygen species ( ROS ) via stimulation of nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase . - pubmed
RIFM fragrance ingredient safety assessment , 2 - nonanone , CAS Registry Number 821 - 55 - 6 . - pubmed
TSLP concentration was measured by ELISA after acetone precipitation of the samples . - pubmed
ACE2 is part of the counter - regulatory renin - angiotensin - aldosterone system and is also expressed in the lower respiratory tract along the alveolar epithelium . - pubmed
WSD exposure also altered ovarian cycles , delaying the time to the E2 surge , decreasing progesterone and anti - Mullerian hormone levels and increasing the number of antral follicles present by ultrasound . - pubmed
H2O2 was supplied exogenously by glucose oxidase , and glucose was produced at a level which was itself noninhibitory . - pubmed
Two phases were distinguished : the first phase was dependent on the oxidation of SCN (- ) by lactoperoxidase and H2O2 , which was reversed by reducing agent , and the second phase was dependent on the presence of accumulated H2O2 , which was reversed by catalase . - pubmed
Pre - operative replacement of haemodialysis by peritoneal dialysis is advisable to facilitate the treatment of cerebral oedema , maintain the electrolyte and water balance and ensure adequate post - operative enteral and parenteral feeding . - pubmed
Specific inhibitors of the endoplasmic - reticulum Ca2 + pump will deplete intracellular stores and are therefore useful to study the role of store depletion on plasma - membrane Ca2 + permeability . - pubmed
There was no significant change in the RNA levels of several other starch biosynthetic enzymes , but there was a great increase in the RNA level of the major sucrose synthesizing enzyme sucrose phosphate synthase . - pubmed
For each stage of the estrous cycle , the enzymatic activities were quantified by means of the 3H2O - release assay in the case of P450AROM and thin - layer chromatography in the case of 5 alpha - reductase . - pubmed
The proportion of L - tryptophan metabolized via the latter flux increased over 10 - fold ( 75 % of total tryptophan metabolized ) as the concentration of L - tryptophan was raised from 5 x 10(-5 ) to 5 x 10(-4 ) M. L - Tryptophan metabolized via the kynureninase flux was less than 5 % of total tryptophan metabolized . - pubmed
2 - 3H liberation increased dose - dependently at tryptophan concentration higher than 10(-5)M , while the kynureninase flux reached its plateau at 10(-5)M. Formation of tryptophol and indole-3 - acetic acid via indole-3 - pyruvic acid and indole-3 - acetaldehyde with indole aldehyde as a by - product was confirmed using exogenous tryptophan metabolites with indole rings . - pubmed
The following findings characterized the poly(ADPR ) reaction : ( 1 ) pretreatment of nuclear extracts with snake venom phosphodiesterase which removes poly ( ADPR ) chains strongly reduced the material which was retained by phenylboronate ; ( 2 ) the ADPR transferase inhibitor benzamide ( 100 microM ) suppressed AO - induced poly(ADP - ribosylation ) ; ( 3 ) poly(ADP - ribosylation ) reduced the electrophoretic mobility of the modified histones . - pubmed
The extent of substitution 30 min after exposure to AO generated by 50 micrograms / mL xanthine and 5 micrograms / mL xanthine oxidase was 0.8 % for A24 greater than 0.3 % for H4 greater than 0.1 % for H3.1 = 0.1 % for H3.2 = 0.1 % for H2B.2 greater than 0.09 % for H2A. - pubmed
Within 60 min , poly ( ADPR ) substitution had decreased to control levels for H3 and H4 and below control levels for H2A and H2B.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
The dyes ethidium bromide ( EB ) , propidium iodide ( PI ) , and acridine orange ( AO ) were used in this study . - pubmed
Hormonal concentrations ( thyrotropin [ TSH ] , prolactin [ PRL ] , thyroxin , triiodothyronine , and reverse triiodothyronine ) before and during a TRH test were assessed in the 2 groups before ( D0 ) and after ( D7 ) each treatment . - pubmed
The TSH ( peak values and Kr ) and PRL ( peak values and AUC ) responses to TRH were significantly decreased in the nifedipine group compared to the placebo group . - pubmed
No relationship existed between urinary NAG and serum creatinine , beta-2 - microglobulin , and degree of metabolic control ( HbA7 ) . - pubmed
p24 is a thyroid protein ( Mr 24,000 ) identified by two - dimensional gel electrophoresis on the basis that its synthesis and phosphorylation are up - regulated by thyrotropin and cyclic AMP agonists . - pubmed
The encoded polypeptide ( 189 residues ) displays a putative target - site for phosphorylation by cyclic AMP - dependent protein kinase and belongs to the superfamily of proteins binding Ca2 + through ' EF hand ' domains . - pubmed
The ability of p24 to bind Ca2 + has been directly confirmed on Western blots . - pubmed
The ubiquitous nature of p24 , together with its regulatory and sequence characteristics suggest that it constitutes an important target common to the cyclic AMP and Ca2 + -phosphatidylinositol cascades . - pubmed
The metR gene was subcloned from pRSE562 , and this plasmid , pMRIII , relieved the methionine auxotrophy of a metR mutant after transformation . - pubmed
The in vitro expression of both genes was inhibited by the MetJ protein and S - adenosylmethionine in the presence or absence of MetR protein . - pubmed
The concentration of protein kinase C was also determined by using [ 3H ] phorbol-12,13 - dibutyrate ( PDBu ) . - pubmed
We found that calcium influx was unaltered under basal conditions but that the ability of norepinephrine ( NE ) to augment influx was significantly depressed ( P less than .05 ; [ control vs. septic , 572 + /- 54 [ SE ] vs. 428 + /- 30 mumol Ca2+/kg dry wt . - pubmed
In conclusion , we found that during sepsis alpha 1 - adrenergic receptor activation of both calcium influx and efflux by NE is decreased ; these alterations could be related to the depressed aortic contractility observed in sepsis . - pubmed
The initial GM PC20 histamine for the group was 22.9 mg / ml , which did not change significantly with two further histamine challenges or when the airways were refractory to AMP , suggesting that loss of AMP effect was not related to loss of contractility of airway smooth muscle or down regulation of H1 - histamine receptors.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
The corticotropin - releasing factor is the predominant component of a complex control system of adrenal cortex secretion , which also includes catecholamines and the antidiuretic hormone . - pubmed
Treatment of cells with dibutyryl adenosine 3 ' : 5 '- cyclic monophosphate ( 1 mmol / l ) or forskolin ( 5 mumol / l ) increased the levels of cytosolic GH mRNA by between 1.6- and 4.7 - fold . - pubmed
These data demonstrate that cyclic adenosine nucleotides may mediate the GRF induction of GH gene transcription . - pubmed
Cerebrospinal fluid ( CSF ) glycine levels did not change during treatment , suggesting inadequate penetration into the central nervous system of glycine at the dosage used . - pubmed
Before , during and after this treatment , serum levels of aMT , PRL , LH , FSH and testosterone were examined . - pubmed
Although aMT increased at least 1,500 - fold over basal levels , only PRL was significantly and consistently elevated after aMT treatment , whereas serum levels of the other hormones were not altered . - pubmed
Serum PRL levels were significantly higher after aMT than after placebo ; GH showed a slight but not significant trend towards elevation after aMT , whereas other hormones were not altered . - pubmed
An acute pharmacological dose of aMT causes isolated elevation of serum PRL levels and may slightly increase GH . - pubmed
Effects of bromocriptine on prolactin cellular hypertrophy , proliferation and secretory activity in diethylstilbestrol - induced pituitary tumors . - pubmed
BC treatment of tumors reversed the effects of DES on pituitary weight , PRL content and in vitro PRL release . - pubmed
The results establish that PRL cell hypertrophy , as well as hyperplasia , results from DES treatment . - pubmed
Bromocriptine treatment reverses this hypertrophy concomitant with inhibiting PRL synthesis and release . - pubmed
The distribution of Copper - Zinc containing and Manganese - containing superoxide dismutase in the liver , kidney , interscapular brown adipose tissue ( IBAT ) and brain of the ground squirrel , as well as the effect of hibernation , was studied . - pubmed
TEER was inversely correlated with 22Na transport ( r2 = 0.93 , P = less than 0.001 ) but not with 125I - LDL transport . - pubmed
Removal of beta - VLDL from the culture medium resulted in the return of cellular cholesterol content and LDL transport to control values . - pubmed
The permanganate method , the immunoperoxidase method , and a newly developed autoclave method were used to distinguish different types of amyloid fibril proteins in formalin - fixed , paraffin - embedded tissue sections . - pubmed
On the other hand , prealbumin type systemic amyloidosis and senile plaques of SDAT were resistant to both permanganate oxidation and prolonged autoclaving . - pubmed
[ Studies on the structure of bovine serum kininogen , using cyanogen bromide and carboxypeptidase B ] . - pubmed
Maximal oxygen consumption , pulmonary function , body composition , and anthropometry of adolescent female athletes . - pubmed
In vivo staining of cytoskeletal actin by autointernalization of nontoxic concentrations of nitrobenzoxadiazole - phallacidin . - pubmed
Although cells tend to exclude the fluorescent toxin , reducing the internal concentration by approximately 1,000 times , some of it enters the cells , probably by pinocytosis , and stains actin structures at low intracellular NBD - Ph concentrations ( approximately 5 - 15 nM ) , where cell toxicity was negligible or at least not detectable by phase - contrast microscopy . - pubmed
Derivatives of cytochrome c having S - methyl cysteine and ethionine substituted for methionine residue 80 have been synthesized in order to study the effects of structural perturbations near the heme on the biological function of cytochrome c. - pubmed
The etionine derivative has 96 % as much activity as native cytochrome c in the succinate oxidase system , whereas the S - methyl cysteine analog is totally inactive . - pubmed
Epinephrine acutely mediates skeletal muscle insulin resistance . - pubmed
In this study we investigated the influence of epinephrine on insulin - mediated glucose uptake by peripheral tissue . - pubmed
Epinephrine - induced skeletal muscle insulin resistance may play a major role in insulin - resistant states and may contribute to accelerated protein catabolism seen following injury . - pubmed
The polymorphisms of alcohol and aldehyde dehydrogenase and their significance for acetaldehyde toxicity . - pubmed
The feasibility of quantifying end point histochemistry and relating oxidative staining density ( reduced nicotinomide adenine dinucleotide diaphorase : NADH - D ) to whole body maximal oxygen consumption ( VO2 max ) was also investigated , while muscle fiber types , classified according to alkaline APTase stains , were studied and related to muscle oxidative capacity ( succinate dehydrogenase : SDH ) , VO2 max and " in vivo " torque - velocity properties . - pubmed
While results confirm earlier reports on skeletal muscle properties and performance it was concluded that end point histochemistry could be reliably quantified and that an " oxidative " stain such as NADH - D correlates extremely well with VO2 max ( r = 0.86 , p less than 0.001 ) whereas correlations between % slow twitch fibres ( Alkaline ATPase stain ) and VO2 max were lower ( r = 0.44 , p less than 0.05 ) . - pubmed
Furthermore , in type IV allergic model , CM - ext had the inhibitory effects on induction phase and effector phase in picryl chloride - induced contact dermatitis ( PC - CD ) . - pubmed
It solved the analytical problem that artefact TBC and MTBC are formed during analysis . - pubmed
The calibration graphs showed a good linearity in the range 0.1 - 50.0 ng ml-1 of urine samples spiked with standard TBC and MTBC . - pubmed
Protein purity was assessed by silver - stained native and sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS PAGE ) . - pubmed
The curve relating Vt to conditioning potential had a negative slope ( which was insensitive to addition of 1 mM cobalt or replacement of calcium with 20 mM CaEGTA ) between -60 and -35 mV and a steep positive slope with further depolarization Unexpectedly , fibers became inactivatable with less conditioning depolarization in soleus than in EDL when Vt was measured with 50 msec test pulses . - pubmed
The aim of this study was to know about the plasmatic variations of insulin and its potential origin in the obesity with or without abnormal tolerance to glucose . - pubmed
Early transient incapacity and cerebral radiation syndrome were shown to result from the de - energizing of the brain due to NAD and ATP neuron pools depletion since DNA breaks stimulate the repair enzyme , ADP - ribosyltransferase , that consumes NAD as a substrate . - pubmed
In contrast to thermolabile prenyltransferases , which have four to six cysteine residues , the thermostable farnesyl diphosphate synthase carries only two cysteine residues . - pubmed
This enzyme is also unique in that some of the amino acids that are fully conserved in equivalents from other sources are replaced by functionally different amino acids . - pubmed
GAL4 - p53 chimeras constructed using naturally occurring p53 mutations at either codon 141 ( Tyr-141 ) or 175 ( His-175 ) of p53 had little ability to activate the reporter gene ; in contrast , mutations at either codon 248 ( Trp-248 ) or 273 ( His-273 ) produced greater transcriptional activities than did wild - type p53 . - pubmed
Surprisingly , coexpression of either p53 ( Trp-248 ) or ( His-273 ) with the GALA - p53 ( wild - type , His-273 , Trp-248 , His-175 , Tyr-141 ) effectors conferred an increase in transcriptional activation as compared with the effector alone . - pubmed
p53 ( Tyr-141 / His-273 ) behaved as a dominant negative mutant and inhibited the ability of the combination of p53 ( His-273 ) and GAL4 - p53 ( C - terminus ) to stimulate the reporter gene . - pubmed
Double immunoprecipitation by sequentially using GAL4 and p53 antibodies showed that p53 ( His-273 ) and ( Tyr-141 / His-273 ) , but not p53 ( Tyr-141 ) , can efficiently oligomerize in vivo to the C - terminal region of p53 . - pubmed
Transcriptional activating function of p53 may be modulated by oligomerization ; some mutations , such as His-273 and Trp-248 , participate in these functions. - pubmed
Proof of this reaction is found in addition of superoxide dismutase ( SOD ) to the system , which totally eliminates the contribution of the Trp . - pubmed
The entrainment with melatonin was restricted to a narrow window for melatonin to be applied , since injections at CT8 did not result in entrainment . - pubmed
Lack of association between polymorphisms in the 5 - HT2A receptor gene and the antipsychotic response to clozapine . - pubmed
OBJECTIVE : The authors ' goal was to determine whether either of two 5 - HT2A receptor polymorphisms , 102 - T / C and 452 - His / Tyr , are associated with clozapine response . - pubmed
Our study revealed that surgical interventions in lung adenocarcinomas resulted in a marked ( P & lt ; or = 0.01 ) and long - lasting ( 5 days ) release of neopterin and sIL-2 - R postoperatively . - pubmed
Regulation of tyrosine hydroxylase gene expression during hypoxia : role of Ca2 + and PKC . - pubmed
Gene expression for tyrosine hydroxylase ( TH ) , the rate - limiting enzyme in catecholamine biosynthesis , is regulated by reductions in oxygen tension ( hypoxia ) . - pubmed
Based on the evidence that the transcription factors that bind to the TH promoter during hypoxia can also be induced by elevations in cytosolic Ca2 + , the role of Ca2 + in the hypoxic regulation of the TH gene was explored . - pubmed
The addition of the L - type calcium channel blockers nifedipine or verapamil caused partial inhibition of the hypoxia - induced increase in TH mRNA . - pubmed
Importantly , chelation of extracellular Ca2 + completely inhibited the increase in TH mRNA by hypoxia . - pubmed
Addition of chelerythrine chloride ( CHL ) , a protein kinase C inhibitor , to the media before exposure to hypoxia also resulted in an inhibition of TH induction by hypoxia . - pubmed
These results suggest that hypoxia regulates TH gene expression by a mechanism that is dependent on influx of calcium from the extracellular stores , partially but not exclusively through the L - type calcium channels . - pubmed
RBCK2 , a cDNA fragment related to RBCK1 was obtained , that lacks the 161 - bp sequence of RBCK1 and encodes 260 amino acid residues . - pubmed
The 240 - amino acid sequence in the NH2 - terminal of RBCK2 is identical with RBCK1 and contains two coiled - coil regions but no other structural motifs , whereas the 20 - amino acid sequence in the COOH - terminal is distinct from RBCK1 . - pubmed
Biexciton formation in GaAs / AlAs type - II superlattices under extremely low excitation powers . - pubmed
PAI-1 antigen levels measured in conditioned media after a 19 - hour incubation period of adipose tissue explants were significantly correlated with plasma PAI-1 antigen levels ( r=0.54 , P=0.004 ) and with systemic lipid parameters such as triglycerides and high density lipoprotein cholesterol ( r=0 . - pubmed
Phage display was used to identify peptides that interact specifically with either estradiol- or tamoxifen - activated estrogen receptor alpha . - pubmed
When these peptides were coexpressed with estrogen receptor alpha in cells , they functioned as ligand - specific antagonists , indicating that estradiol - agonist and tamoxifen - partial agonist activities do not occur by the same mechanism . - pubmed
The ability to regulate estrogen receptor alpha transcriptional activity by targeting sites outside of the ligand - binding pocket has implications for the development of estrogen receptor alpha antagonists for the treatment of tamoxifen - refractory breast cancers . - pubmed
Virologic and CD4 + cell responses to new nucleoside regimens : switching to stavudine or adding lamivudine after prolonged zidovudine treatment of human immunodeficiency virus infection . - pubmed
Both regimens significantly increased CD4 + cell numbers , the means of weeks 40 and 48 rose to 49 and 36 CD4 + cells per cubic millimeter among zidovudine plus lamivudine and stavudine recipients , respectively . - pubmed
It has been suggested that the cystic fibrosis transmembrane conductance regulator ( CFTR ) chloride channel may utilize a novel gating mechanism in which open and closed states are not in thermodynamic equilibrium . - pubmed
We demonstrate that CFTR channel gating occurs in the absence of ATP hydrolysis and hence does not depend on an input of free energy from this source . - pubmed
These observations demonstrate that phosphorylated CFTR behaves as a conventional ligand - gated channel employing cytoplasmic ATP as a readily available cytoplasmic ligand ; under physiological conditions ligand hydrolysis provides efficient reversibility of channel opening . - pubmed
Allopregnanolone is a well - established allosteric modulator of the GABAA receptor but its physiological roles within the nervous system remain unclear . - pubmed
Protein kinase C and guanosine triphosphate combine to potentiate calcium - dependent membrane fusion driven by annexin 7 . - pubmed
Exocytotic secretion is promoted by the concerted action of calcium , guanine nucleotide , and protein kinase C. - pubmed
We now show that the calcium - dependent membrane fusion activity of annexin 7 in vitro is further potentiated by the combined addition of guanine nucleotide and protein kinase C. - pubmed
Guanosine triphosphate ( GTP ) and its non - hydrolyzable analogues optimally enhance the phosphorylation of annexin 7 by protein kinase C in vitro . - pubmed
Reciprocally , phosphorylation by protein kinase C significantly potentiates the binding and hydrolysis of GTP by annexin 7 . - pubmed
In vivo , the ratio of bound GDP / GTP and phosphorylation of annexin 7 change in direct proportion to the extent of catecholamine release from chromaffin cells in response to stimulation by carbachol , or to inhibition by various protein kinase C inhibitors . - pubmed
These results thus lead us to hypothesize that annexin 7 may serve as a common site of action for calcium , guanine nucleotide , and protein kinase C in the exocytotic membrane fusion process in chromaffin cells . - pubmed
The State Scale of Dissociation ( SSD ) was administered with other psychiatric scales ( DES , BDI , BAI , SCI - PANSS ) to 130 participants with DSM - IV major depressive disorder schizophrenia , alcohol withdrawal , dissociative disorders and controls . - pubmed
All three forms of structural remodeling of the hippocampus are mediated by hormones working in concert with excitatory amino acids ( EAA ) and N - methyl - D - aspartate ( NMDA ) receptors . - pubmed
EAA and NMDA receptors are also involved in neuronal death that is caused in pyramidal neurons by seizures and by ischemia and prolonged psychosocial stress . - pubmed
FK506 is an immunophilin - binding ligand that inhibits calcineurin and decreases nitric oxide ( NO ) production in the nervous tissues . - pubmed
tolerance and dependence and compared them with the effects of the non - specific NO synthase inhibitor , NG - nitro - L - arginine methyl ester ( L - NAME ) , and specific inducible NO synthase inhibitor , aminoguanidine . - pubmed
Decarboxylases involved in polyamine biosynthesis and their inactivation by nitric oxide . - pubmed
Ornithine decarboxylase is a PLP - requiring decarboxylase , whereas S - adenosylmethionine decarboxylase ( AdoMetDC ) contains a covalently bound pyruvate prosthetic group . - pubmed
These residues , Cys360 in ornithine decarboxylase ( ODC ) and Cys82 in AdoMetDC , react readily with nitric oxide ( NO ) , which is therefore a potent inactivator of polyamine synthesis . - pubmed
oryzae beta - galactosidase ) in the initial content of SH groups in the lactases . - pubmed
lactis beta - galactosidase with thiopropyl - agarose than for the same reduction with DTT . - pubmed
oryzae beta - galactosidase , nearly twice as many micromoles of SH groups per milligram of protein were needed with thiopropylagarose than with DTT . - pubmed
Disulfide bonds in beta - lactoglobulin were not accessible to thiopropyl - agarose , since this reduction was only possible in the presence of 6 M urea . - pubmed
These results proved that highly substituted thiopropyl - agarose is as good a reducing agent as DTT , for the reduction of disulfide bonds in proteins . - pubmed
Free fatty acid ( FFA ) concentrations in cerebrospinal fluid ( CSF ) are recognized as markers of brain damage in animal studies . - pubmed
Higher concentrations of total polyunsaturated fatty acids ( P<0.001 ) and of arachidonic , myristic and palmitic acids measured individually in CSF ( P<0.01 ) obtained 1 week after the insult were associated with a worse outcome at the time of hospital discharge using the Glasgow Outcome Scale . - pubmed
This preliminary investigation suggests that CSF FFA concentrations may be useful as a predictive marker of outcome following TBI . - pubmed
Alkaline phosphatase in leucocytes by an azo dye method . - pubmed
Serum and erythrocyte folate and serum vitamin B12 was determined by radioassay ; serum ferritin by enzimoimmunoassay ; hemoglobin were performed by semi - automated method . - pubmed
Improved HPLC method for carbohydrate - deficient transferrin in serum . - pubmed
BACKGROUND : There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate - deficient transferrin ( CDT ) can be traceable . - pubmed
METHODS : Transferrin was iron - saturated by mixing the serum with ferric nitrilotriacetic acid , and lipoproteins were precipitated with dextran sulfate and calcium chloride . - pubmed
Quantification relied on selective absorbance of the iron - transferrin complex at 470 nm . - pubmed
Serum and the pretreated samples were stable for at least 2 days at 22 or 4 degrees C. Sera from 132 healthy controls contained [ mean ( SD ) ] 1.16 ( 0.25)% disialotransferrin , 4.77 ( 1.36)% trisialotransferrin , 80.18 ( 2.01)% tetrasialotransferrin , and 13.88 ( 1.69)% pentasialo - + hexasialotransferrin . - pubmed
BDCM treatment reduced DC and GAL activities and elevated NR and AR activity . - pubmed
GAL , AR , and NR activities were significantly different after treatment with bromate , chloroform , BDCM , and MX , but not the mixture . - pubmed
They have shown that high concentrations of ethanol lead to necrosis , while low concentrations induce apoptosis due to activation of Fas - receptors . - pubmed
Triggering of apoptosis through Fas - receptors represents a mechanism of action different from that observed with high concentrations of ethanol , thus , reducing the complications that follow the inflammatory process due to necrosis . - pubmed
Mean TRPV6 expression was the same , but there was evidence of different responsiveness to 1,25 ( OH )( 2 ) D ( 3 ) . - pubmed
In the GG genotype group , but not the AG , duodenal TRPV6 expression increased with 1,25 ( OH )( 2 ) D ( 3 ) . - pubmed
We postulate that lower levels of expression of VDR in the GG group produce greater sensitivity to 1,25 ( OH )( 2 ) D ( 3 ) . - pubmed
We then explored why these cells with arsenite - induced DNA damage were arrested at mitosis instead of G2 stage . - pubmed
Immunoblot analysis confirmed that arsenite treatment reduced the X - irradiation - induced phosphorylation of both ataxia - telangiectasia , mutated at serine 1981 and Cdc25C at serine 216 , events which are crucial for G2 checkpoint activation and G2 arrest . - pubmed
Synthesis , inhibition properties , and theoretical study of the new nanomolar trehalase inhibitor 1 - thiatrehazolin : towards a structural understanding of trehazolin inhibition . - pubmed
1 - Thiatrehazolin is a very potent , slow , tight - binding trehalase inhibitor . - pubmed
A structural model for trehalase inhibition by trehazolin and its analogues , based on the experimental results and supported by theoretical calculations , is proposed . - pubmed
Time - resolved fluorescence experiments using tryptophan excitation have shown that RET is manifested by a distinct growing in of acceptor fluorescence at a rate characteristic for this process . - pubmed
The method is based on methanol - modified carbon dioxide as the mobile phase at flow rate of 3.0 ml / min with elution through a JASCO Finepak SIL-5 , [ C18 ( 5 - micron , 25 cm x 4.6 mm , i.d . ) - pubmed
Nifedipine gastrointestinal therapeutic system ( GITS ) is a long - acting formulation of a calcium channel blocker . - pubmed
The combination of vasostatin gene therapy and cyclophosphamide administration improved therapeutic effects in melanoma tumours . - pubmed
These observations led the authors to conclude that the identity of BIC is dubious , most probably it will represent a subtype of BPEI . - pubmed
Current data now suggest that caveolin-1 plays an essential role in the regulation of liver regeneration and that it is implicated in the regulation of triglyceride accumulation , which is an essential process that plays a critical role in the regulation of liver regeneration . - pubmed
Methylation of estrogen receptor alpha and mutL homolog 1 in normal colonic mucosa : association with folate and vitamin B-12 status in subjects with and without colorectal neoplasia . - pubmed
OBJECTIVE : We tested the hypothesis that biomarkers of folate and vitamin B-12 status are associated with estrogen receptor alpha ( ERalpha ) and mutL homolog 1 ( MLH1 ) promoter methylation in subjects with and without neoplasia . - pubmed
DESIGN : Biopsies of normal - appearing colorectal mucosa from 156 subjects with and without colorectal neoplasia ( disease free , n = 76 ; cancer , n = 28 ; adenoma , n = 35 ; hyperplastic polyps , n = 17 ) were obtained at colonoscopy and used to evaluate methylation in 7 CpG sites in the ERalpha promoter and 13 CpG sites in the MLH1 promoter . - pubmed
Methylation indexes were generated to reflect an average methylation value across all CpG dinucleotides in both ERalpha and MLH1 . - pubmed
The ERalpha methylation index correlated negatively with serum vitamin B-12 ( r = -0.239 , P = 0.003 ) and positively with plasma homocysteine ( r = 0.188 , P = 0.021 ) . - pubmed
Disease status ( P & lt ; 0.005 ) , age ( P & lt ; 0.001 ) , and serum vitamin B-12 concentrations ( P = 0.006 ) were independent determinants of ERalpha promoter methylation . - pubmed
Serum and red cell folate concentrations had no influence on ERalpha promoter methylation . - pubmed
CONCLUSION : Serum vitamin B-12 but not folate status may be associated with ERalpha promoter methylation in normal - appearing colorectal mucosa . - pubmed
Five variables including the Child - Pugh score , albumin , bilirubin , ascites , and HV waveform significantly correlated with the survival in a univariate analysis . - pubmed
RESULTS : Mutation frequencies for rifampicin ( MIC & gt;or= 0.2 mg / L ) did not differ significantly between serovars L(2 ) and D ( 5.7 x 10(-7 ) versus 6.3 x 10(-7 ) ) . - pubmed
Moxifloxacin resistance of all serovar L(2 ) clones depended on single - nucleotide point mutations in the quinolone resistance - determining region of the gyrA , whereas no additional mutations were found in the gyrB , parC or parE genes . - pubmed
We examined whether the risk of TGCC is associated with serum levels of 11 organochlorine pesticides , including p , p '- DDE , and whether the p , p '- DDE - TGCC association is modified by CAG or GGN repeat polymorphisms in the androgen receptor gene . - pubmed
We observed no clear patterns between TGCC risk and concentrations of any of the organochlorines measured , nor did we observe that the risk associated with p , p '- DDE was modified by androgen receptor CAG ( & lt;23 versus & gt ; or = 23 repeats ) or GGN ( & lt;17 versus & gt ; or = 17 repeats ) genotype . - pubmed
Ferritin formation was the highest in cells treated with SFP compared to those treated with other iron compounds or chelates . - pubmed
Adding ascorbic acid ( AA ) and cysteine to SFP in a 20:1 molar ratio increased ferritin formation by 3- and 2 - fold , respectively , whereas adding citrate had no significant effect on the bioavailability of SFP . - pubmed
The proposed KDRI includes 14 donor and transplant factors , each found to be independently associated with graft failure or death : donor age , race , history of hypertension , history of diabetes , serum creatinine , cerebrovascular cause of death , height , weight , donation after cardiac death , hepatitis C virus status , human leukocyte antigen - B and DR mismatch , cold ischemia time , and double or en bloc transplant . - pubmed
The effects of beta - elemene on the expression of BCL-2 , caspase-3 , DR-4 and NF - kappaB P65 proteins were analyzed by Western blot . - pubmed
A variable basic cleft on the top of the dimer interface binds to phosphatidylinositide and regulates the intracellular localization of PDCD10 . - pubmed
Of these genes , valB , which encodes a sugar nucleotidyltransferase , was found through inactivation study to be essential for validamycin biosynthesis , but its role was unclear . - pubmed
To characterize the role of ValB in validamycin biosynthesis , four carbasugar phosphate analogues were synthesized and tested as substrate for ValB. - pubmed
The results showed that ValB efficiently catalyzes the conversion of valienol 1 - phosphate to its nucleotidyl diphosphate derivatives , whereas other unsaturated carbasugar phosphates were found to be not the preferred substrate . - pubmed
ValB requires Mg ( 2 + ) , Mn ( 2 + ) , or Co ( 2 + ) for its optimal activity and uses the purine - based nucleotidyltriphosphates ( ATP and GTP ) more efficiently than the pyrimidine - based NTPs ( CTP , dTTP , and UTP ) as nucleotidyl donor . - pubmed
While LPA exerted no significant effect on the cell migration , the prior treatment of the cells with LPS or IL-1beta resulted in the appearance of migration activity in response to LPA . - pubmed
The LPS induction of the migration response to LPA was associated with the production of IL-1beta precursor protein and inhibited by the IL-1 receptor antagonist . - pubmed
The IL-1beta treatment also allowed LPA to activate Rac1 . - pubmed
The LPA - induced Rac1 activation and migration were inhibited by pertussis toxin , a small interfering RNA specific to LPA ( 1 ) receptors , and LPA ( 1 ) receptor antagonists , including Ki16425 . - pubmed
However , the IL-1beta treatment had no appreciable effect on LPA(1 ) receptor mRNA expression and LPA - induced activation of ERK , Akt , and proliferation . - pubmed
The induction of the migration response to LPA by IL-1beta was inhibited by a constitutively active RhoA. - pubmed
The aminolevulinic acid dehydratase activity , hematocrit , and hemoglobin tended to be higher in group E than S. The iron content in the flexor hallucis longus muscle was significantly higher in E than S , whereas the content in the liver , spleen , kidney , and heart did not significantly differ between the groups . - pubmed
We measured serum concentrations of insulin like growth factor - I ( IGF - I ) in 20 thalassemic males with short stature ( height SDS & lt;-2 ) and/or slow growth velocity ( GV & lt;-1 SD ) throughout their childhood and adolescence , compared these data with normal reference data validated in our lab , and evaluated their growth hormone secretion in response to clonidine and glucagon stimulation . - pubmed
The method was evaluated by investigating the effect of roll speed for two pharmaceutical excipients with distinctive material properties : microcrystalline cellulose ( MCC ) and di - calcium phosphate dihydrate ( DCPD ) . - pubmed
It was found that the maximum pressure and nip angle for DCPD , which is a cohesive powder , decrease sharply with increasing roll speed whereas they are essentially independent of roll speed for MCC , which is an easy flowing powder . - pubmed
Sialoadhesin is a macrophage adhesion molecule containing 17 extracellular immunoglobulin - like domains , and is an I - type lectin which binds to sialic acid ligands expressed on hematopoietic cells . - pubmed
In this study , a new treatment strategy was proposed consisting of a combination of 5,7 - dimethoxycoumarin ( DMC ) , an activator of melanogenesis , and valproic acid ( VPA ) , a well - known drug that is one of the histone deacetylase inhibitors ( HDACis ) . - pubmed
VPA and DMC also induced the synthesis of melanin and the formation of dendrite and star - shaped cells . - pubmed
Tyrosinase gene expression and tyrosinase activity significantly increased in response to VPA treatment . - pubmed
CNTs with linear poly ( ethylene glycol ) amphiphiles trigger the lectin pathway of the complement through both L - ficolin and mannan - binding lectin recognition . - pubmed
An amphiphile with branched poly ( ethylene glycol ) architecture also activated the lectin pathway but only through L - ficolin recognition . - pubmed
High temperature / light and freeze - thaw treatments verified direct emissions of the isoprene oxidation products MVK and MACR together with the first observations of 3 - methyl furan ( 3 - MF ) and 2 - methyl-3 - buten-2 - ol ( MBO ) as putative novel isoprene oxidation products . - pubmed
Mechanical wounding also stimulated emissions of MVK and MACR . - pubmed
Three viruses ( 0.9 % ) were found to be oseltamivir resistant due to an H275Y mutation in the neuraminidase gene . - pubmed
In addition , GRb1 also increased the activity of lactate dehydrogenase and superoxide dismutase in POFS . - pubmed
Palonosetron hydrochloride is a longer - acting selective 5 - HT3 receptor antagonist that has been approved for the prevention of chemotherapy - induced nausea and vomiting and is being evaluated for the prevention of postoperative nausea and vomiting . - pubmed
These hydride transfers are mediated by B ( C6F5 ) 3 via two SN2 processes without involving 1 . - pubmed
Comparison of the deduced amino acid sequences of NptA among strains resulted in strain grouping similar to that based on transcript abundance . - pubmed
Contribution of hot spring bacterial consortium in cadmium and lead bioremediation through quadratic programming model . - pubmed
In vitro , IQGAP1 mRNA and protein expression were observed to decline under HG media at 48 h. But phosphorylation of ERK1 / 2 was activated under HG media at 24 h and 48 h. However , HG - induced downregulation of IQGAP1 protein was attenuated by specific ERK1 / 2 activation inhibitor PD98059 . - pubmed
The controlled release of low - dose ( 1 mu g and 5 mu g ) G - CSF significantly enhanced new bone formation when combined with a beta - TCP disc . - pubmed
Moreover , administration of 5 mu g G - CSF using a controlled release system significantly promoted the biodegradable properties of beta - TCP . - pubmed
In conclusion , the controlled release of 5 mu g G - CSF significantly enhanced the osteoconductive and biodegradable properties of beta - TCP . - pubmed
The combination of G - CSF slow - release and beta - TCP is a novel and promising approach for treating pediatric craniofacial bone defects . - pubmed
During brain development , polysialylated ( polySia ) neural cell adhesion molecules ( polySia - NCAMs ) modulate cell - cell adhesive interactions involved in synaptogenesis , neural plasticity , myelination , and neural stem cell ( NSC ) proliferation and differentiation . - pubmed
These differentiated cells were immunoreactive with mAb 's to polySia , NCAM , beta - III tubulin , nestin , S-100 and stained with BrdU. - pubmed
Autosomal dominant hypercholesterolemia ( ADH ) characterized by markedly elevated LDL cholesterol levels that leads to premature morbidity and mortality from atherosclerotic cardiovascular disease ( ASCVD ) . - pubmed
Familial combined hyperlipidemia ( FCHL ) characterized by variable elevations of total cholesterol , triglycerides , or LDL cholesterol and a high risk of premature ASCVD . - pubmed
Pregnancy management : In a woman with the APOE p. Leu167del variant , triglyceride levels should be measured when pregnancy is identified and at least one to two times later in pregnancy as severe hypertriglyceridemia ( & gt;500 mg / dL ) in pregnancy increases the risk for pancreatitis and fetal and maternal death . - pubmed
Acetylcholinesterase is a serine protease that rapidly hydrolyzes neurotransmitter acetylcholine . - pubmed
Their implications were studied using specific inhibitors ( U0126 , mitogen - activated protein kinase kinase , MEK , inhibitor and C compound , AMPK inhibitor ) . - pubmed
Both U0126 and C compound almost completely prevented LPS - induced TNF - alpha production . - pubmed
INTRODUCTION : Both testosterone therapy and chronic treatment with phosphodiesterase type 5 inhibitors ( PDE5Is ) have positive effects on the histology of penile corpora and erectile function . - pubmed
Inhibition of calpain prevents manganese - induced cell injury and alpha - synuclein oligomerization in organotypic brain slice cultures . - pubmed
Overexposure to manganese has been known to promote alpha - synuclein oligomerization and enhance cellular toxicity . - pubmed
However , the exact mechanism of Mn - induced alpha - synuclein oligomerization is unclear . - pubmed
After slices were treated with 400 muM Mn for 24 h , there were significant increases in the percentage of apoptotic cells , lactate dehydrogenase release , intracellular [ Ca2+]i , calpain activity , and the mRNA and protein expression of calpain 1 and alpha - synuclein . - pubmed
These results also showed that calpain inhibitor II pretreatment could reduce Mn - induced nerve cell injury and alpha - synuclein oligomerization . - pubmed
These findings revealed that Mn induced the cleavage of alpha - synuclein protein via overactivation of calpain and subsequent alpha - synuclein oligomerization in cultured slices . - pubmed
Moreover , the cleavage of alpha - synuclein by calpain 1 is an important signaling event in Mn - induced alpha - synuclein oligomerization . - pubmed
The Full Scale IQ was not significantly different between groups ( high - DHA 98.3 , SD 14.0 , standard - DHA 98.5 , SD 14.9 ; mean difference adjusted for sex , birthweight strata and hospital -0.3 , 95 % CI -2.9 to 2.2 ; p=0.79 ) . - pubmed
Click Chemistry in Lead Optimization of Boronic Acids as beta - Lactamase Inhibitors . - pubmed
Boronic acid transition - state inhibitors ( BATSIs ) represent one of the most promising classes of beta - lactamase inhibitors . - pubmed
This synthetic pathway , which avoids intermediate purifications , proved to be flexible and efficient , affording in good yields a panel of 14 BATSIs bearing three different R1 amide side chains ( acetamido , benzylamido , and 2 - thienylacetamido ) and several R substituents on the triazole . - pubmed
Homology modeled structure analysis demonstrated that cecropin - TY1 adopted two alpha - helices ( Leu3 - Thr24 , Ile27 - Leu38 ) linked by a hinge ( Leu25 - Pro26 ) and the structure surface was partly positively charged . - pubmed
METHODS : Fourteen population clusters of approximately 1000 residents each in Zambia 's Luangwa and Nyimba districts , which had high pre - existing usage rates ( 81.7 % ) of pyrethroid - impregnated LLINs were quasi - randomly assigned to receive IRS with either of two pyrethroids , namely deltamethrin [ Wetable granules ( WG ) ] and lambdacyhalothrin [ capsule suspension ( CS ) ] , with an emulsifiable concentrate ( EC ) or CS formulation of the organophosphate pirimiphos methyl ( PM ) , or with no supplementary vector control measure . - pubmed
RESULTS : Supplementation of LLINs with PM CS offered the greatest initial level of protection against malaria in the first 3 months of application ( incremental protective efficacy ( IPE ) [ 95 % confidence interval ( CI ) ] = 0.63 [ CI 0.57 , 0.69 ] , P & lt ; 0.001 ) , followed by lambdacyhalothrin ( IPE [ 95 % CI ] = 0.31 [ 0.10 , 0.47 ] , P = 0.006 ) and PM EC ( IPE , 0.23 [ CI 0.15 , 0.31 ] , P & lt ; 0.001 ) and then by deltamethrin ( IPE [ 95 % CI ] = 0.19 [ -0.01 , 0.35 ] , P = 0.064 ) . - pubmed
Neither pyrethroid formulation provided protection beyond 3 months after spraying , but the protection provided by both PM formulations persisted undiminished for longer periods : 6 months for CS and 12 months for EC . - pubmed
The CS formulation of PM provided greater protection than the combined pyrethroid IRS formulations throughout its effective life IPE [ 95 % CI ] = 0.79 [ 0.75 , 0.83 ] over 6 months . - pubmed
A key drug for treatment of EGFR mutation - positive non - small cell lung cancer is epidermal growth factor receptor - tyrosine kinase inhibitor ( EGFR - TKI ) . - pubmed
The median progression - free survival in the gefitinib group and the erlotinib group was 22.4 months and 14.1 months , respectively , and the median overall survival was 30.5 months and 32.4 months , respectively . - pubmed
Although SOD and GSH were significantly higher in spermatic vein compared to median cubital vein , TAC was significantly higher in median cubital vein . - pubmed
Involvement of GABAA receptors in 5 - HT1A and sigma1 receptor synergism on prefrontal dopaminergic transmission under circulating neurosteroid deficiency . - pubmed
RATIONALE : We previously reported that the fluvoxamine - induced increase in prefrontal dopamine levels is enhanced by adrenalectomy / castration ( which results in circulating neurosteroid deficiency ) , via combined activation of serotonin1A ( 5 - HT1A ) and sigma1 receptors . - pubmed
Picrotoxin also potentiated the increase in prefrontal dopamine levels mediated by co - administration of the 5 - HT1A receptor agonist osemozotan and the sigma1 receptor agonist ( + ) - SKF-10,047 , while it did not affect the co - administration - induced changes in noradrenaline and serotonin levels . - pubmed
Conversely , the GABAA receptor agonist diazepam ( 1 mg / kg ) blocked the effect of adrenalectomy / castration on the fluvoxamine - induced increase in prefrontal dopamine levels . - pubmed
CONCLUSIONS : These results suggest that the GABAA receptor plays a key role in mediating the synergistic effects of 5 - HT1A and sigma1 receptor activation on prefrontal dopamine neurotransmission . - pubmed
In vivo PET / CLI imaging clearly reveals SLNs as early as 1 h post PEG - RIe - AuNP - injection , with peak signals achieved at 6 h postinjection , which is consistent with the biodistribution results . - pubmed
In vitro , like ERS inhibitor 4 - phenylbutyric acid ( PBA ) , HDL inhibited ox - LDL- or tunicamycin ( TM , an ERS inducer)-induced increase in visfatin and resistin secretion . - pubmed
Moreover , HDL inhibited ox - LDL - induced free cholesterol ( FC ) accumulation in whole cell lysate and in the endoplasmic reticulum . - pubmed
Additionally , like PBA , HDL inhibited ox - LDL- or TM - induced activation of ERS response as assessed by the decreased phosphorylation of protein kinase - like ER kinase and eukaryotic translation initiation factor 2alpha and reduced nuclear translocation of activating transcription factor 6 as well as the downregulation of Bip and CHOP . - pubmed
YB1 and NOD2 co - migrated on sucrose gradient centrifugation , and GMDP addition led to the formation of higher molecular mass complexes containing both YB1 and NOD2 . - pubmed
Co - immunoprecipitation demonstrated a direct interaction between YB1 and NOD2 , a major recombinant fragment of NOD2 ( NACHT - LRR ) bound to YB1 , and complex formation was stimulated by GMDP . - pubmed
A method based on the analysis of trimethylsilyl ( TMS ) derivatives by capillary gas chromatography electrospray ionization mass spectrometry ( GC - ESI / MS ) was proposed . - pubmed
BACKGROUND : Artemisinin combination therapy ( ACT ) is used worldwide as the first - line treatment against uncomplicated Plasmodium falciparum malaria . - pubmed
Despite the success of ACT in reducing the global burden of malaria , the emerging of resistance to artemisinin threatens its use . - pubmed
flux ( Y1 ) , lag time ( Y2 ) , diffusion coefficient ( Y3 ) , permeability coefficient ( Y4 ) , and input rate ( Y5 ) of clotrimazole gel formulations . - pubmed
Moreover , PTS was able to increase capillary densities and enhance angiogenesis in ischemic boundary zones and up - regulate vascular endothelial growth factor ( VEGF ) and Angiopoietin-1 ( Ang-1 ) expression by activating the Shh signaling pathway . - pubmed
Here , structure - activity relationship of rMnSODSeq was evaluated on the basis of its activity and stability in the presence of inhibitors , NaCl , denaturants , detergents , reducing agents , and at different pH values . - pubmed
The distribution of brominated flame retardants ( BFRs ) including 8PBDEs , DBDPE , BTBPE , EH - TBB , BEH - TEBP and PBEB in road dust ( RD ) collected in Xinxiang , China was characterized . - pubmed
Of the 13 analyzed substances , BDE-209 and BEH - TEBP were the most abundant components in RD from Xinxiang . - pubmed
Similar amounts of BDEs excluding BDE-209 were found at different types of sampling sites , and thus , atmospheric deposition is also a probable source of BFRs in RD which can be subject to air transportation . - pubmed
Our results confirm that the use of deca - BDE commercial mixture is a major source of PBDE contamination in RD . - pubmed
Cathepsins are lysosomal peptidases belonging to the papain family , and based on their catalytic sites , these enzymes can be divided into serine , cysteine and aspartic proteases . - pubmed
The wear resistance of the Zr - Al - Fe - Nb alloys in air and phosphate buffer saline ( PBS ) solution is superior to that of pure Zr . - pubmed
The wear mechanism of Zr - based alloys sliding in air is controlled by oxidation and abrasive wear whereas that sliding in PBS is controlled by synergistic effects of the abrasive and corrosive wear . - pubmed
Electrochemical measurements demonstrate that the Zr - based alloys are corrosion resistant in PBS . - pubmed
The Zr75Al5Fe10Nb10 exhibits the best corrosion resistance in PBS , which contributes to its superior wear resistance in a simulated body environment . - pubmed
The potencies of PFASs to inhibit SRD5A1 are PFOS & gt ; PFOA & gt ; PFHxS = PFBS , with IC50 values of PFOS and PFOA of 1.92 + - 0.07 and 14.24 + - 0.07 muM and no effects for PFHxS and PFBS at 100 muM , respectively . - pubmed
The potencies of PFASs to inhibit AKR1C9 and RoDH2 are PFOS & gt ; PFOA & gt ; PFHxS = PFBS , with IC50 values of PFOS and PFOA on these two enzymes about 100 muM and no effects for PFHxS and PFBS at 100 muM , respectively . - pubmed
Upon identifying a lead hit from this screen KEA1 - 97 , we used activity - based protein profiling ( ABPP)-based chemoproteomic platforms to identify that this compound targets lysine 72 of thioredoxin - a site previously shown to be important in protein interactions with caspase 3 to inhibit caspase 3 activity and suppress apoptosis . - pubmed
It is classified as MPS type III , though it is caused by four different genetic defects , determining subtypes A , B , C and D. In each subtype of MPS III , the primary storage GAG is heparan sulfate ( HS ) , but mutations leading to A , B , C , and D subtypes are located in genes coding for heparan N - sulfatase ( the SGSH gene ) , alpha - N - acetylglucosaminidase ( the NAGLU gene ) , acetyl - CoA : alpha - glucosaminide acetyltransferase ( the HGSNAT gene ) , and N - acetylglucosamine-6 - sulfatase ( the GNS gene ) , respectively . - pubmed
DiR " hotspots " were specifically associated with CD206 - expressing M2 macrophages in the spinal cord and this was confirmed by co - localization with anti - CD63 antibodies labeling a tetraspanin characteristically expressed on exosomes . - pubmed
Herein , we show that single intra - medial forebrain bundle ( MFB ) injection of 6 - hydroxydopamine ( 6 - OHDA ) efficiently induced long - term activation of GSK-3beta and reduced NSC self - renewal , proliferation , neuronal migration , and neuronal differentiation accompanied with increased astrogenesis in subventricular zone ( SVZ ) and hippocampal dentate gyrus ( DG ) . - pubmed
Ethanol exposure inhibits hepatocyte lipophagy by inactivating the small guanosine triphosphatase Rab7 . - pubmed
Nutrient starvation results in Rab7 activation on the surface of the LD and lysosomal compartments , resulting in the mobilization of triglycerides stored within the LDs for energy production . - pubmed
Here , we examine whether the steatotic effects of alcohol exposure are a result of perturbations to the Rab7 - mediated lipophagic pathway . - pubmed
Conclusion : A mechanism is supported whereby ethanol exposure inhibits Rab7 activity , resulting in the impaired transport , targeting , and fusion of the autophagic machinery with LDs , leading to an accumulation of hepatocellular lipids and hepatic steatosis . - pubmed
The interaction mechanism of phenol and PAHs in aqueous and sludge phases was systematically studied in two full - scale engineering projects composed of anaerobic - oxic - oxic ( A - O1 - O2 ) and anaerobic - oxic - hydrolytic - oxic ( A - O1 - H - O2 ) sequences . - pubmed
Thus , these results suggested that in vivo1H MRS can be used to reliably investigate the glutamate system . - pubmed
The size of the Protein Data Bank ( PDB ) has increased more than 15 - fold since 1999 , which enabled the expansion of models that aim at predicting enzymatic function via their amino acid composition . - pubmed
One such system is the standard genetic code ( SGC ) , which generally minimizes the cost of amino acid replacements resulting from mutations or mistranslations . - pubmed
This approach allowed us to place the SGC in the global space of possible codes , which is a more appropriate and unbiased comparison than that with randomly generated codes because they are characterized by relatively uniform amino acid assignments to codons . - pubmed
Hyoscyamine is converted to 6beta - hydroxyhyoscyamine ( anisodamine ) and scopolamine by hyoscyamine 6beta - hydroxylase ( H6H ) , a 2 - oxoglutarate dependent dioxygenase . - pubmed
Furthermore , four critical hormones of salicylic acid ( SA ) , jasmonic acid ( JA ) , ethylene ( ET ) and abscisic acid ( ABA ) had higher levels in 1/3TP , 2/3TP and TP than those in NP . - pubmed
CONCLUSIONS : Tx - ESRD patients have worse quality metrics related to anemia , phosphorus , albumin , and vascular access compared to Nat - ESRD patients . - pubmed
He presented to us one year later with a right neck draining sinus tract , reaching the carotid sheath on CT scan . - pubmed
RESULTS : All melatonin treatments reduced chilling injury symptoms , reduced electrolyte leakage , malondialdehyde ( MDA ) content , H2 O2 and superoxide anion ( O2 - ) , and increased proline content and the activity of superoxide dismutase ( SOD ) , catalase ( CAT ) , and g - aminobutyric acid ( GABA ) , and reduced the activities of phospholipase D ( PLD ) and lipoxygenase ( LOX ) compared to the control . - pubmed
Treatment with oridonin for 24 h increased apoptosis by 4.1 % , and increased the protein levels of Bax and cleaved caspase-3 but significantly decreased the levels of IL-1beta in the culture supernatant ( p & lt ; 0.05 ) . - pubmed
In addition , 6 h of oridonin treatment significantly decreased the number of GFP - LC3 punctate dots and inhibited the protein levels of ATG5 and Beclin1 by 80.01 % and 42.12 % , respectively . - pubmed
Acute inflammatory pericarditis secondary to cardiac sarcoidosis evaluated by 18F - fluorodeoxyglucose PET / CT imaging . - pubmed
DOX increased the malondialdehyde level and decreased the catalase , superoxide dismutase activities and glutathione levels ; MP prevented these alterations . - pubmed
The control of extracellular ATP and adenosine levels is done by ectonucleotidases , which represent a potential target to be evaluated in the progression of cardiovascular diseases . - pubmed
AREAS COVERED : The fetal safety / risks of clomiphene citrate , aromatase inhibitors , metformin , gonadotropins and progestins are discussed . - pubmed
In the multivariate analysis , increasing largest tumor dimension ( p < 0.01 ) and sinus invasion ( p < 0.01 ) predicted Simpson grade IV resection , whereas skull base location predicted neurological morbidity ( p = 0.02 ) . - pubmed
A RESPONSE TO " THE POTENTIAL LONG - TERM COMPARATIVE EFFECTIVENESS OF LAROTRECTINIB AND ENTRECTINIB FOR SECOND - LINE TREATMENT OF TRK FUSION - POSITIVE METASTATIC LUNG CANCER " . - pubmed
These responses were secondary to sPRR - His evoked elevations in Nox4 - derived H2O2 production that resulted in inflammation , apoptosis and reduced NO production . - pubmed
Each of these sPRR - His - evoked responses was attenuated by AT1R inhibition using Losartan ( Los ) but not ACE inhibition using captopril ( Cap ) . - pubmed
Further mechanistic exploration revealed that sPRR - His activated AT1R downstream Gq signaling pathway . - pubmed
Immunoprecipitation coupled with autoradiography experiments and radioactive ligand competitive binding assays indicate sPRR directly interacts with AT1R via Lysine199 and Asparagine295 . - pubmed
SRC family kinase ( SFK ) inhibitor dasatinib improves the antitumor activity of anti - PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation . - pubmed
In in vitro assays , we demonstrated that dasatinib blocks proliferation and transforming growth factor beta - driven conversion of effector CD4 + cells into Tregs through targeting of phospholymphocyte - specific protein tyrosine kinase and downstream effectors pSTAT5 and pSMAD3 . - pubmed
Dasatinib synergizes with anti - PD-1 to impair tumor growth in NSCLC experimental models . - pubmed
A comparison of the substrate and electron - donor specificities of the methane mono - oxygenases from three strains of methane - oxidizing bacteria . - pubmed
The optimized energies of seven beta - bends , repeating C5 and C7 , and right- and left - handed alpha - helical conformations for each of eight tetrapeptides have been computed using empirical methods . - pubmed
Activation of bovine factor X in the presence of calcium , magnesium , barium or manganese ion . - pubmed
Inhibition by acetylene of conventional hydrogenase in nitrogen - fixing bacteria . - pubmed
It outflowed from the determination of selected serum proteins ( Ig and acute phase proteins ) and salivary lysozyme that the group under the combined influence of formaldehyde and toluene showed significantly lower IgG and higher alpha-1 - antitrypsin ( A1AT ) . - pubmed
The group at risk of toluene was characteristical in elevated concentrations of alpha-2 - macroglobulin ( A2 M ) and A1AT . - pubmed
Salivary lysozyme concentration of apprentice environmentally exposed to formaldehyde in the noon showed the decrease , whereas its increase occurred in controls with the difference on 5 % significancy level . - pubmed
Serum triglycerides and lipoprotein cholesterol were measured before and after the trip . - pubmed
With the four different diets used , a standard diet ( 3800 kcal day-1 , 26 % fat , 260 mg cholesterol day-1 ) , a low - energy diet ( 2300 kcal day-1 , 21 % fat , 110 mg cholesterol day-1 , a high - fat - high - cholesterol diet ( 3800 kcal day-1 , 52 % fat , 480 mg cholesterol day-1 ) and a high - cholesterol diet ( 3800 kcal day-1 , 29 % fat , 410 mg cholesterol day-1 ) , a decrease in very low - density lipoprotein - low - density lipoprotein ( VLDL - LDL ) fraction by 38 % , 50 % , 41 % , and 54 % , respectively , was obtained . - pubmed
No significant increase in high - density lipoprotein ( HDL ) cholesterol was found in the experiments with the standard diet and the low - energy diet , but in the high - fat - high - cholesterol diet experiment the HDL cholesterol increased by 19 % and the ratio HDL cholesterol : total cholesterol from 0.327 to 0.490 . - pubmed
With the high - cholesterol diet an increase in HDL cholesterol of about the same extent was obtained . - pubmed
Titration of the average Ca2 + concentration showed that the Ca2 + affinity for cholecystokinin-8 secretion was higher than that for amino acids . - pubmed
The predicted v - rel protein ( p59v - rel ) has 14 amino acid substitutions and 3 amino acid deletions relative to the predicted turkey c - rel protein . - pubmed
The 5 ' Rev env - derived amino - terminus of p59v - rel also has amino acid substitutions in codons 3 , 6 , and 9 relative to wild - type Rev - A env . - pubmed
To distinguish the critical alterations from the neutral ones , we made a mutation that resulted in a substitution of the 5 ' Rev env - derived amino - terminus by a single methionine , as well as several back - mutations , in the context of an otherwise wild - type v - rel and tested the transforming , immortalizing , and tumorigenic properties of the resulting proteins . - pubmed
Absence of the 5 ' Rev env - derived amino - terminus , as well as back - mutations of six of the amino acid changes present in the rel - derived amino - terminal and central regions of v - rel , reduced transformation efficiency . - pubmed
IL-1 beta ( 0.3 micrograms / kg ) administered 24 h before induction of colitis increased PGE2 ( 231 + /- 36 to 1,299 + /- 572 pg / ml , P less than 0.01 ) and reduced subsequent inflammatory cell infiltration index ( from 2.8 + /- 0.3 to 1.4 + /- 0.3 , P less than 0.02 ) and edema ( from 2.5 + /- 0.3 to 1.3 + /- 0.3 , P less than 0.01 ) compared with vehicle - matched animals . - pubmed
together with IL-1 beta prevented the stimulation of PGE2 and the reduction in inflammation . - pubmed
These data demonstrate that pretreatment with IL-1 beta 24 h before the induction of colitis reduces inflammation by a mechanism that requires prostaglandin synthesis . - pubmed
Since BM 42.304 decreased mitochondrial acylcarnitine and increased the cytosolic concentration significantly , BM 42.304 was presumed to inhibit mitochondrial acylcarnitine translocase . - pubmed
This effect of aprotinin may be a consequence of : renal kallikrein inhibition which prevents augmentation of renal kinins in response to increased vasopressin levels , or other unrecognized properties of aprotinin . - pubmed
2 urine samples collected 10 and 13 days after Glucantime treatment revealed only antibody ( IgG ) , not soluble antigen . - pubmed
Direct measurements of phosphorylation of the Ca2 + ATPase of the sarcoplasmic reticulum ( SR ) have shown that the lifetime of the first phosphorylated intermediate in the Ca2 + transport cycle , E1 approximately P , increases with decreasing [ Mg2 + ] ( Dupont , Y. - pubmed
54:669 - 678 ) under high [ Mg2 + ] conditions ( 25 mM ) indicated that changes in the profile structure of the SR membrane could be responsible for the low - temperature transient trapping of E1 approximately P that occurs at temperatures below 2 - 3 degrees C , the upper characteristic temperature th for lipid lateral phase separation in the membrane . - pubmed
We now present results of our study of the Ca2 + uptake kinetics and of the structure of the SR membrane at low [ Mg2 + ] ( less than or equal to 100 microM ) . - pubmed
Our results show a slowing in the kinetics of both phases of the Ca2 + uptake process and an increase in the duration of the plateau of the fast phase before the onset of the slow phase , indicating an increase in the lifetime ( transient trapping ) of E1 approximately P. Calcium uptake kinetics at low [ Mg2 + ] and moderately low temperature ( approximately 0 degree C ) are similar to those observed at much lower temperatures ( approximately -10 degrees C ) at high [ Mg2 + ] . - pubmed
Also , at the lower [ Mg2 + ] the upper characteristic temperature th for lipid lateral phase separation was found to be higher , at approximately 8 - 10 degrees C. Our studies indicate that both temperature and [ Mg2 + ] affect the structure and the functionality ( as measured by changes in the kinetics of Ca2 + uptake ) of the SR membrane . - pubmed
Membrane lipid phase behavior and changes in the Ca2 + ATPase profile structure seem to be related , and we have found that structural changes are responsible for the slowing of the kinetics of the fast phase of Ca2 + uptake , and could also mediate the effect that [ Mg2 + ] has on E1 approximately P lifetime . - pubmed
Urine was collected in several fractions on each study day and the urine volume , excretion of ofloxacin , and excretion of creatinine , alanine aminopeptidase , N - acetyl - beta - D - glucosaminidase , and gamma - glutamyltransferase were calculated in each fraction collected . - pubmed
The effects of hydroxyurea have been investigated on three events of the cell cycle , S - phase , mitosis , and the cyclic synthesis of thymidine kinase , in the synchronous plasmodium of the myxomycete Physarum . - pubmed
Hydrolysis with phospholipase established that phytanate was confined to the 1 - position of CGP . - pubmed
The effect of urea on salt - induced changes in histone IV . - pubmed
The effect on actin ATPase of phalloidin and tetramethylrhodamine phalloidin . - pubmed
Actin polymerization has been studied in the absence of excess nucleotide . - pubmed
Using G - actin ATP monomers , it was shown that mechanical shearing stimulates ATP hydrolysis . - pubmed
The procedures used enabled the detection of differential effects of phalloidin and tetramethylrhodamine - phalloidin , on the P(i)-release step of the actin ATPase . - pubmed
It is concluded that tetramethylrhodamine , in contrast to phalloidin , accelerates P(i)-release from actin filaments . - pubmed
These tissues require insulin for facilitated transport of glucose and express the insulin - responsive transporter GLUT4 . - pubmed
The present results indicate that simultaneous measurement of the levels of urinary gamma - Gla and plasma prothrombin and protein C is a useful tool for the diagnosis of liver diseases and diabetes mellitus . - pubmed
This in vitro release of LAC was abolished when samples were stored on ice until centrifugation within 5 h. In contrast , a statistically significant increase in ELA - PI of 10 % was observed following storage on ice for 2 h of blood specimens drawn from healthy persons . - pubmed
EDTA - plasma obtained by venous puncture following minimal stasis contained 10 % higher concentrations of LAC compared to samples drawn from intravenous catheters , while no difference was observed in the case of ELA - PI . - pubmed
A structure - activity study of fatty acid interaction with mitochondrial uncoupling protein . - pubmed
Fatty acid ( FA ) uniport via mitochondrial uncoupling protein ( UcP ) was detected fluorometrically with PBFI , potassium - binding benzofuran phthalate and SPQ , 6 - methoxy - N -( 3 - sulfopropyl )- quinolinium , indicating K+ and H + , respectively . - pubmed
The FA structural patterns required for FA flip - flop , UcP - mediated FA uniport , activation of UcP - mediated H + transport in proteoliposomes , and inhibition of UcP - mediated Cl- uniport by FA , were identical . - pubmed
DMXB ( 100 microM ) activated alpha7 homo - oligomeric receptors , without significant activity at alpha2- , alpha3- and alpha4 - containing subtypes . - pubmed
Iron contents of the brain , activities of monoamine oxidase ( MAO ) in the corpus striatum , and the contents of monoamine neurotransmitter and its metabolite in the cerebral cortex and hippocampus were determined by DCP - AES technique , enzyme histochemical method , and high performance liquid chromatography with electrochemical detection ( HPLC - ECD ) , respectively . - pubmed
Modulation of the cellular metabolism of cytarabine and fludarabine by granulocyte - colony - stimulating factor during therapy of acute myelogenous leukemia . - pubmed
Previous in vitro investigations demonstrated that human leukemia cells , when incubated with hematopoietic growth factors such as granulocyte - colony - stimulating factor ( G - CSF ) , augment the accumulation of the triphosphate 1 - beta - D - arabinofuranosylcytosine ( ara - C cytarabine ) . - pubmed
To test whether G - CSF infusion prior to ara - C infusion would biologically modulate the accumulation of ara-9 - beta - D - arabinofuranosylcytosine 5 '- triphosphate ( ara - CTP ) and other ara nucleotides in the leukemia blasts during therapy , protocols were designed to infuse G - CSF prior to fludarabine ( 9 - beta - D - arabinofuranosyl-2 - fluoroadenine monophosphate ) and ara - C to increase the accumulation of the active triphosphates [ 9 - beta - D - arabinofuranosyl-2 - fluoroadenine 5 '- triphosphate ( F - ara - ATP ) and ara - CTP ] in acute myelogenous leukemia ( AML ) blasts during therapy . - pubmed
To complement these in vivo studies , ex vivo accumulation of ara - CTP was also investigated before and after G - CSF infusion . - pubmed
The AUC of ara - CTP , on the other hand , was not affected ( median , 1.0 - fold ; range , 1.0 - 1.2 ; P = 0.571 ) after G - CSF infusion . - pubmed
Consistent with these in vivo investigations , ex vivo ara - CTP accumulation was decreased in the AML blasts after G - CSF infusion . - pubmed
Based on these data it could be concluded that ( a ) infusion of G - CSF before fludarabine augmented the rate of F - ara - ATP synthesis in circulating AML blasts during therapy , suggesting that G - CSF may benefit fludarabine therapy by biological modulation ; ( b ) G - CSF did not increase ara - CTP accumulation , rather it may have caused it to decrease ; and ( c ) these data imply that when G - CSF and ara - C are used in combination , administration of fludarabine prior to ara - C may maintain the ara - CTP AUC . - pubmed
The structure of tubulin shows paclitaxel ( Taxol(R ) ) binding to a pocket in beta tubulin on the microtubule 's inner surface , which counteracts the effects of GTP hydrolysis occurring on the other side of the monomer . - pubmed
The re - activated I ( min ) had the same kinetic properties as I(min ) when it is activated by Ca ( 2 +)-mobilizing agonists in intact cells and by IP ( 3 ) in excised plasma membrane patches and it was inhibited by the I ( crac ) inhibitor SKF95365 . - pubmed
These data support the possible role of SHMT in the fetal control of plasma serine levels . - pubmed
While cSHMT may contribute to fetal hepatic serine production , its activity pattern does not support a primary role in the control of fetal hepatic serine biosynthesis . - pubmed
In the placenta , mSHMT may be important for glycine production from serine . - pubmed
The twin arginine motif in its signal peptide and its sole integration requirement of a DeltapH suggested that plastid fusion / protein translocation factor employs the DeltapH pathway . - pubmed
Thus , DeltapH pathway machinery facilitates both signal peptide - directed and N - tail - mediated membrane integration and does not strictly require the twin arginine motif . - pubmed
Carbamoyl phosphate synthetase ( CPS ) catalyzes one of the most remarkable reactions ever described in biological chemistry , in which carbamoyl phosphate is produced from one molecule of bicarbonate , two molecules of Mg2 + ATP , and one molecule of either glutamine or ammonia . - pubmed
Instead it is the composition of the coat protein domain from amino acid 123 to 149 , as a minimal transmission domain , with the contribution of amino acids 149 to 174 for efficient transmission . - pubmed
UL97 is able to autophosphorylate and to phosphorylate certain exogenous substrates , including nucleoside analogs such as ganciclovir . - pubmed
It has previously been concluded that phosphorylation of UL97 is essential for its phosphorylation of ganciclovir . - pubmed
Glutathione S - transferase - UL97 fusion protein purified from insect cells was found to be already partially phosphorylated , but neither extensive autophosphorylation nor phosphatase treatment meaningfully altered the time course of its phosphorylation of the exogenous substrate , histone H2B. Sequencing and mass spectrometric analyses of ( 32 ) P - labeled tryptic peptides of the UL97 fusion protein identified nine sites of autophosphorylation , all within the first 200 residues of the protein , outside of conserved protein kinase subdomains . - pubmed
Types 1 and 2 11beta - HSD messenger RNA ( mRNA ) and protein in placentas obtained at 121 to 185 days ' gestation ( dGA , term approximately 185 dGA , n = 16 ) , during labor between 141 and 193 dGA ( n = 8) , and after maternal administration of four doses of 87.5 microg / kg betamethasone ( n = 5 ) at 12 - hour intervals at 121 to 135 dGA were analyzed by Northern and Western blot . - pubmed
Maternal betamethasone administration dramatically increased ( P & lt;.05 ) 11beta - HSD 2 mRNA as well as protein without effect on 11beta - HSD 1 mRNA and protein expression . - pubmed
Decreased placental 11beta - HSD 2 may enhance maternal cortisol passage to the fetus at the end of gestation , thereby contributing to cortisol - mediated changes within the placenta and cortisol in fetal plasma at this stage of fetal development . - pubmed
The positive effect of betamethasone on placental 11beta - HSD 2 induction further suggests an ability of the placenta to regulate glucocorticoid transfer in the presence of elevated maternal glucocorticoid . - pubmed
27 and 28 were analyzed for 2BTCA using a solid - phase microextraction - GC - MS method . - pubmed
The increase in invertase activity coincided with the rapid accumulation of glucose and fructose that reached 8 - fold the control leaf value . - pubmed
In addition , acid vacuolar invertase activity appeared to be highly correlated with xylem sap ABA concentration . - pubmed
In order to investigate the nature of the relationship between ABA and invertase activity , and to disconnect ABA from a likely sucrose side - effect , excised leaves were supplied with ABA or sucrose . - pubmed
As a consequence of ABA supply , a peak in leaf ABA appeared 4 h later which was followed by an enhancement of vacuolar invertase activity . - pubmed
The transcript level of the Ivr2 gene encoding one vacuolar invertase presented the same two peaks pattern as leaf ABA , with a 2 h lag . - pubmed
Thus , ABA appeared to be a powerful enhancer of the IVR2 vacuolar invertase activity and expression . - pubmed
A COMPONENT SPLITTING DIISOPROPYLFLUOROPHOSPHATE IN PREPARATIONS OF SENDAI AND NEWCASTLE DISEASE VIRUSES AND IN A FRACTION FROM NORMAL CHORIOALLANTOIC MEMBRANE CELLS ; ITS POSSIBLE IDENTITY WITH HAEMOLYSIN . - pubmed
AIMS : To determine the cost to the NHS of prescribed low - dose aspirin . - pubmed
Serious adverse events were uncommon but despite this aspirin cost the NHS between 6 and 25 times the cost of aspirin tablets due to dispensing costs and the cost of managing adverse effects . - pubmed
The effects of GABA and GABAa receptor antagonist were observed in this experimental study . - pubmed
Numerous histological and electrophysiological studies have been performed on the contribution of GABA to the auditory pathway ; however , the spatio - temporal patterns of excitatory propagation and the relationships between GABA receptor and excitatory propagation have yet to be reported . - pubmed
Bicuculline methiodide ( BMI ) , a competitive antagonist against GABAa receptor , partially reversed the effects of GABA on the optical signals . - pubmed
4,4 '- Dithiodipyridine ( PySSPy ) monolayers on Au ( 111 ) were investigated by cyclic voltammetry , X - ray photoelectron spectroscopy ( XPS ) and in situ scanning tunneling microscopy ( STM ) . - pubmed
Metoprolol and propranolol ( BCS class I ) as well as hydrochlorothiazide ( BCS class III ) were selected as model drugs . - pubmed
The effect of methyl viologen on the flux of the intermediate compounds was investigated by measuring the activity of enzymes related to PHB biosynthesis , glucose 6 - phosphate dehydrogenase ( G6PD ) , and NADPH level . - pubmed
Instead , a threonine residue at position 15 in the hKv1.5 N - terminus is critically important . - pubmed
Mutation of this residue , which lies within a consensus site for phosphorylation by protein kinase C , to an alanine , completely abrogated the effect of SAP97 on channel expression . - pubmed
Although we were unable to detect phosphorylation of this residue , specific inhibition of kinase C by Calphostin C eliminated the increase in wild - type hKv1.5 currents associated with SAP97 overexpression suggesting a role for this kinase in the response . - pubmed
Dye - coupling analysis revealed that each mutant exhibits a dominant - negative effect on wild - type Cx43 . - pubmed
Caspase-3- and calpain - mediated tau cleavage are differentially prevented by estrogen and testosterone in beta - amyloid - treated hippocampal neurons . - pubmed
Our results indicated that estrogen and testosterone prevented caspase-3- and calpain - mediated tau cleavage , respectively . - pubmed
Thus , estrogen decreased the levels of caspase-3 - cleaved 50 - kDa truncated tau , while testosterone prevented the generation of a calpain - cleaved 17 - kDa tau fragment . - pubmed
Furthermore , our findings indicated that testosterone was more effective than estrogen in protecting hippocampal neurons against Abeta - induced cell death . - pubmed
Collectively , our data suggest that preventing the decline of estrogen and testosterone associated with normal aging might reduce the susceptibility of central neurons to Abeta - induced toxicity . - pubmed
Association between cutaneous melanoma , Breslow thickness and vitamin D receptor BsmI polymorphism . - pubmed
BrdU incorporation indicated that cell expansion occurred at multiple stages except SP1 . - pubmed
In addition , PCA also significantly decreased the activity of elevated caspase-3 , indicating that PCA may inhibit apoptosis of NSCs via suppression of the caspase cascade . - pubmed
Knockdown of TxnIP with small interference RNA suggested that TxnIP mediates the glucose - induced impairment of thioredoxin activity . - pubmed
Knockdown of TxnIP also abrogated both glucose - induced 3H - proline incorporation ( a marker of collagen production ) and oxidative stress . - pubmed
Arginine methylation is a widespread post - translational modification of proteins catalyzed by a family of protein arginine methyltransferases ( PRMTs ) . - pubmed
Trypanosomatids lack gene regulation at the level of transcription , instead relying on post - transcriptional control mechanisms that act at the levels of RNA turnover , translation , and editing , all processes that likely involve multiple RNA - binding proteins , which are common targets of arginine methylation . - pubmed
High resolution ion exchange chromatography analysis of methylated substrates reveals that TbPRMT7 is a type III PRMT , catalyzing the formation of only monomethylarginine , thereby representing the only exclusively type III PRMT identified to date . - pubmed
RESULTS : Steatosis was associated significantly with donor age , increased body mass index ( BMI ) , presence of type II diabetes , ultrasonographic features , heavy alcohol consumption , transaminases [ aspartate - aminotransferase and alanine - aminotransferase ( ALT ) ] , gamma - glutamyl - transpeptidase , and glucose blood levels . - pubmed
The combination of BMI , elevation of ALT , presence of type II diabetes , history of heavy alcohol consumption , and ultrasonography signs of steatosis could identify steatosis & gt;30 % accurately with an AUC of 0.86 ( 95 % CI = 0.81 - 0.91 ) . - pubmed
The effect of dobutamine on AFC and the expression of aquaporin-1 and aquaporin-5 were examined . - pubmed
Lipopolysaccharide administration results in significant lung injury with impaired AFC , while dobutamine improves alveolar fluid reabsorption with elevation of aquaporin-1 and aquaporin-5 . - pubmed
Our study indicates that dobutamine may enhance alveolar fluid reabsorption by increasing the expression of aquaporin-1 and aquaporin-5 . - pubmed
Rosuvastatin , C - reactive protein , LDL cholesterol , and the JUPITER trial . - pubmed
Pumilio 2 controls translation by competing with eIF4E for 7 - methyl guanosine cap recognition . - pubmed
Using deletion analysis , we have mapped the cap interaction domain of Pum2 to the amino terminus of the protein and identified a conserved tryptophan residue that mediates this specific interaction . - pubmed
Human serum albumin consists of a single polypeptide of 585 amino acid residues with 1 Trp residue . - pubmed
CONCLUSION : Despite the selection of tumors expressing c - Kit , imatinib did not appear to delay disease progression after response to chemotherapy . - pubmed
Structural role of the conserved cysteines in the dimerization of the viral transmembrane oncoprotein E5 . - pubmed
For productive interaction , E5 needs to dimerize via a C - terminal pair of cysteines , though a recent study suggested that its truncated transmembrane segment can dimerize on its own . - pubmed
Comparison by circular dichroism in detergent micelles and lipid vesicular dispersion and by NMR in trifluoroethanol demonstrates that the absence of one or both cysteines does not influence the highly alpha - helical secondary structure , nor does it impair the ability of E5 to dimerize , observations that are further supported by sodium dodecylsulfate polyacrylamide gel electrophoresis . - pubmed
Oriented circular dichroism in lipid bilayers shows that E5 is aligned as a transmembrane helix with a slight tilt angle , and that this membrane alignment is also independent of any cysteines . - pubmed
Vitamin A directly correlated with pre - albumin levels at 6 weeks ( r = 0.67 , P & lt ; 0.001 ) and 1 - year ( r = 0.67 , P & lt ; 0.0001 ) . - pubmed
There was no correlation between the roux limb length measurement and pre - albumin or vitamin A serum concentrations at these post - operative follow - ups . - pubmed
Administration of Gd - DPTA resulted in margin enhancement on T1 - weighted images . - pubmed
Mitoxantrone prior to interferon beta-1b in aggressive relapsing multiple sclerosis : a 3 - year randomised trial . - pubmed
RESULTS : The time to worsen by at least one Expanded Disability Status Scale point confirmed at 3 months was delayed by 18 months in the MITOX group compared with the IFN group ( p<0.012 ) . - pubmed
The 3 - year risk of worsening disability was reduced by 65 % in the MITOX group relative to the IFN group ( 11.8 % vs 33.6 % ) . - pubmed
CONCLUSIONS : Although there were limitations in this investigator - academic - driven study , the data do suggest that mitoxantrone induction therapy prior to INF beta-1b may have a role in aggressive disease . - pubmed
We fabricated [ Co / Cu ] multilayers revealing a giant magnetoresistance ( GMR ) effect on free - standing elastic poly ( dimethylsiloxane ) ( PDMS ) membranes . - pubmed
The GMR performance of [ Co / Cu ] multilayers on rigid silicon and on free - standing PDMS is similar and does not change with tensile deformations up to 4.5 % . - pubmed
This remarkable performance upon stretching relies on a wrinkling of GMR layers on top of the PDMS membrane . - pubmed
The stimulatory G protein alpha - subunit ( G ( s ) alpha ) couples hormone and other receptors to the generation of intracellular cAMP . - pubmed
Thus , excess GLP-1 action ( or at least through GLP-1R ) does not contribute to the LGsKO metabolic phenotype , and other unknown factors involved in the cross talk between the liver G(s)alpha / cAMP pathway and pancreatic islet function need to be further elucidated . - pubmed
Taken together , MD-2 is a molecular target in the anti - inflammatory action of 1D10G. - pubmed
The lowest cut - off value derived from receiving operator characteristic discriminating Serie A - B from Lega - Pro was 1300 m. The ARIET performance was significantly correlated with VO(2max ) ( r = 0.78 , P & lt ; 0.001 ) , % HR(max ) after 4 min of ARIET ( r = - 0.81 , P & lt ; 0.001 ) and Yo - Yo IR1 performance ( r = 0.95 , P & lt ; 0.001 ) , but not sprint performance ( r = -0.15 ; P = 0.58 ) . - pubmed
Furthermore , middle and high NaF significantly reduced the mRNA expressions of P1 and P2 , and P1 / P2 ratio , whereas the sperm histones level was increased , suggesting the abnormal histone - protamine replacement . - pubmed
When the inhibitory role of chemerin on steroidogenesis was further examined in vitro , chemerin suppressed FSH - induced progesterone and estradiol secretion in cultured preantral follicles and granulosa cells . - pubmed
( + ) - SKF 10047 ( N - allyl - normetazocine ) is a prototypic and specific sigma-1 receptor agonist that has been used extensively to study the function of sigma-1 receptors . - pubmed
( + ) - SKF 10047 inhibits K (+ ) , Na (+ ) and Ca2 + channels via sigma-1 receptor activation . - pubmed
We found that ( + ) - SKF 10047 inhibited Na ( V ) 1.2 and Na ( V ) 1.4 channels independently of sigma-1 receptor activation . - pubmed
The sigma-1 receptor antagonists BD 1063,BD 1047 and NE-100 did not block the inhibitory effects of ( + ) - SKF-10047 . - pubmed
Blocking of the PKA , PKC and G - protein pathways did not affect ( + ) - SKF 10047 inhibition of Na ( V ) 1.2 channel currents . - pubmed
The sigma-1 receptor agonists Dextromethorphan ( DM ) and 1,3 - di - o - tolyl - guanidine ( DTG ) also inhibited Na ( V ) 1.2 currents through a sigma-1 receptor - independent pathway . - pubmed
The ( + ) - SKF 10047 inhibition of Na ( V ) 1.2 currents was use- and frequency - dependent . - pubmed
Point mutations demonstrated the importance of Phe(1764 ) and Tyr(1771 ) in the IV - segment 6 domain of the Na ( V ) 1.2 channel and Phe(1579 ) in the Na ( V ) 1.4 channel for ( + ) - SKF 10047 inhibition . - pubmed
AIM : Previous studies have shown that uric acid ( UA ) has strong anti - oxidant properties , and that high circulating levels of UA are prospectively associated with improved muscle function and cognitive performances in elderly Caucasians . - pubmed
CONCLUSION : The observed differences among the cytotoxicity of AH Plus , EndoREZ , and Epiphany did not reach a significant level at comparable time points after exposure . - pubmed
The CYP2C19 * 17 variant is not independently associated with clopidogrel response . - pubmed
BACKGROUND : Cytochrome P450 2C19 ( CYP2C19 ) is the principal enzyme responsible for converting clopidogrel into its active metabolite , and common genetic variants have been identified , most notably CYP2C19 * 2 and CYP2C19 * 17 , that are believed to alter its activity and expression , respectively . - pubmed
OBJECTIVE : We evaluated whether the consequences of the CYP2C19 * 2 and CYP2C19 * 17 variants on clopidogrel response were independent of each other or genetically linked through linkage disequilibrium ( LD ) . - pubmed
PATIENTS / METHODS : We genotyped the CYP2C19 * 2 and CYP2C19 * 17 variants in 621 members of the Pharmacogenomics of Anti - Platelet Intervention ( PAPI ) Study and evaluated the effects of these polymorphisms singly and then jointly , taking into account LD , on clopidogrel prodrug level , clopidogrel active metabolite level , and adenosine 5 '- diphosphate ( ADP)-stimulated platelet aggregation before and after clopidogrel exposure . - pubmed
In association analyses that did and did not account for the effects of CYP2C19 * 17 , CYP2C19 * 2 was strongly associated with levels of clopidogrel active metabolite ( beta = -5.24 , P = 3.0 x 10(-9 ) and beta = -5.36 , P = 3.3 x 10(-14 ) , respectively ) and posttreatment ADP - stimulated platelet aggregation ( beta = 7.55 , P = 2.9 x 10(-16 ) and beta = 7.51 , P = 7.0 x 10(-15 ) , respectively ) . - pubmed
In contrast , CYP2C19 * 17 was marginally associated with clopidogrel active metabolite levels and ADP - stimulated platelet aggregation before ( beta = 1.57 , P = 0.04 and beta = -1.98 , P = 0.01 , respectively ) but not after ( beta = 0.40 , P = 0.59 and beta = -0.13 , P = 0.69 , respectively ) adjustment for the CYP2C19 * 2 variant . - pubmed
Stratified analyses of CYP2C19 * 2 / CYP2C19 * 17 genotype combinations revealed that CYP2C19 * 2 , and not CYP2C19 * 17 , was the primary determinant in altering clopidogrel response . - pubmed
CONCLUSIONS : Our results suggest that CYP2C19 * 17 has a small ( if any ) effect on clopidogrel - related traits and that the observed effect of this variant is due to LD with the CYP2C19 * 2 loss - of - function variant . - pubmed
Magnetic particle imaging ( MPI ) is a novel tracer - based imaging method detecting the distribution of superparamagnetic iron oxide ( SPIO ) nanoparticles in vivo in three dimensions and in real time . - pubmed
The purpose of this study was to evaluate the angiogenic effect of topical application of three types of ONO-1301 - loaded poly ( lactide - co - glycolide ) microspheres ( ONO-1301 PLGA MS ) . - pubmed
ONO-1301 PLGA MS were prepared with PLGA 5010 , 5020 and 5050 ( with molecular weights of 10 K , 20 K and 50 K , respectively ) , using the solvent evaporation method . - pubmed
Additional in vivo fluorescence imaging showed that PLGA MS remained in the sponge for 28 days . - pubmed
Luminal nutrient sensing by G - protein - coupled receptors ( GPCR ) expressed on the apical domain of enteroendocrine cells activates intracellular pathways leading to secretion of gut hormones that control vital physiological processes such as digestion , absorption , food intake and glucose homeostasis . - pubmed
It has been shown that T1R2 - T1R3 , in association with G - protein gustducin , is expressed in intestinal K and L endocrine cells , where it acts as the intestinal glucose ( sweet ) sensor . - pubmed
A number of studies have demonstrated that activation of T1R2 - T1R3 by natural sugars and artificial sweeteners leads to secretion of glucagon - like peptides 1&2 ( GLP-1 and GLP-2 ) and glucose dependent insulinotropic peptide ( GIP ) . - pubmed
GLP-1 and GIP enhance insulin secretion ; GLP-2 increases intestinal growth and glucose absorption . - pubmed
T1R1 - T1R3 combination co - expressed on the apical domain of cholecystokinin ( CCK ) expressing cells is a luminal sensor for a number of L - amino acids ; with amino acid - activation of the receptor eliciting CCK secretion . - pubmed
This article focuses on the role of the gut - expressed T1R1 , T1R2 and T1R3 in intestinal sweet and L - amino acid sensing . - pubmed
Assessment of differences in the conformational flexibility of hepatitis B virus core - antigen and e - antigen by hydrogen deuterium exchange - mass spectrometry . - pubmed
Here we use hydrogen deuterium exchange - mass spectrometry ( HDX - MS ) to study their structural properties . - pubmed
Cp (- 10 ) 149d undergoes a drastic structural change when the intermolecular disulphide bridge is reduced , adopting a Cp149d -like structure , as evidenced by the detected HDX dynamics being more consistent with Cp149d in many , albeit not all , regions . - pubmed
Whilst Fab E1 binds to Cp149c and Cp149d , it does not bind non - reduced and reduced Cp (- 10 ) 149d , despite unhindered access to the epitope . - pubmed
AIM : The author reported outcomes of a new simplified off - midline technique for closure of the defect after complete excision of the sinus tracts . - pubmed
The most important of these mechanisms is the presence of an inducible erythromycin methylase ( erm ) gene , because it confers macrolide resistance . - pubmed
After that , blood samples were collected to determine blood viscosity , plasma viscosity , haematocrit ( HCT ) , fibrinogen ( FIB ) , blood lipid profile ( including total cholesterol ( TC ) , low - density lipoprotein - cholesterol ( LDL - C ) , triglyceride ( TG ) and high - density lipoprotein - cholesterol ( HDL - C ) ) , nitric oxide ( NO ) , 6 - keto - prostaglandin ( PG ) F1alpha , endothelin ( ET ) and thromboxane ( TX ) B2 . - pubmed
The joint effects of YXC and swimming showed significant changes in the examined parameters : ( 1 ) decreases in plasma viscosity , blood viscosity and FIB ; ( 2 ) increases in NO and 6 - keto - PGF1alpha ; ( 3 ) decreases in ET and TXB2 ; and ( 4 ) decreases in LDL - C and TG . - pubmed
Endovascular treatment consisted of coil and Onyx embolization for sacrifice of the parent vessel proximal to the lesion . - pubmed
Ribosomal S6 kinase 1 ( S6K1 ) phosphorylation , a downstream target of rapamycin ( mTOR)-phosphorylation kinase , increased following ECC + BFR200 as well as ECC . - pubmed
Anti - HA was mixed and interacted with FAM - labeled HA tag ( FAM - E4 ) inside the capillary . - pubmed
The addition of excess E4 or TAMRA - E4 could lead to partial dissociation of the complex and take a two - step mechanism including dissociation and association . - pubmed
In the last few weeks , the FDA approved three new therapies for multiple myeloma : ixazomib , the first oral proteasome inhibitor ; and daratumumab and elotuzumab , two monoclonal antibodies that target CD38 and SLAMF7 , respectively . - pubmed
Design and synthesis of novel Y - shaped barbituric acid derivatives as PPARgamma activators . - pubmed
Fourteen novel compounds were synthesized using hydroxyl group protection - deprotection strategies for PPARgamma activation . - pubmed
BACKGROUND : New Delhi metallo - beta - lactamase-1 ( NDM-1 ) is a relatively recent carbapenemase enzyme that inactivates all beta - lactam antibiotics with the exception of aztreonam . - pubmed
METHODS : Over a period of one year , all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one carbapenem and resistance to at least one third generation cephalosporin were subjected to antibiotic susceptibility testing by disk diffusion and molecular detection of the NDM-1 gene by single - target PCR followed by gel electrophoresis . - pubmed
Our results demonstrated that pretreatment with CGA ameliorated the intestinal mitochondrial injury induced by H2O2 ; membrane potential was increased , mitochondrial swelling , levels of reactive oxygen species , contents of 8 - hydroxy-2 - deoxyguanosine , and cytochrome c released were decreased . - pubmed
The beneficial effects of CGA were accompanied by an increase in antioxidant and respiratory - chain complex I , IV , and V activities . - pubmed
Fatty acid - binding proteins ( Fabp ) belong to a multigene family that are highly conserved in sequence and function , but differ in their gene regulation , suggesting selective pressure is exerted via regulatory elements in the promoter . - pubmed
In vivo comparison of endometrial breakdown induced by progesterone withdrawal to that seen during sustained progesterone exposure , in the presence of NF - kappaB inhibitors , revealed that NF - kappaB - mediated functional progesterone withdrawal is involved in endometrial breakdown in this implant model . - pubmed
The hypervernalization response is achieved by increased enrichments of VIN3 and trimethylation of Histone H3 Lys 27 at the FLC locus without invoking the increased enrichment of Polycomb Repressive Complex 2 . - pubmed
Correction : Dasatinib , a Src inhibitor , sensitizes liver metastatic colorectal carcinoma to oxaliplatin in tumors with high levels of phospho - Src . - pubmed
SEM , TEM , EDX , FTIR , XRD , UV - vis and XPS analyses were employed to characterize the composition , structure , and morphology of ZrO2 - TiO2 hollow spheres . - pubmed
A voltammetric sensor was designed to analyze thiosulfate ( TS ) , 4 - chlorophenol ( 4 - CP ) and nitrite ( NT ) , which are water pollutants , in mixed samples by combining the high conductive properties of NiO nanoparticle ( NiO / NPs ) and the excellent mediator properties of novel catechol derivative ( 2,4 - dimethyl - N '-[ 1 -( 2,3 - dihydroxyphenyl ) methylidene ] aniline ) . - pubmed
2,4 - Dimethyl - N '-[ 1 -( 2,3 - dihydroxyphenyl ) methylidene ] aniline was synthesized for the first time and proposed as a high - quality modifier for electrocatalytic analysis of TS and its simultaneous analysis in the presence of 4 - CP and NT . - pubmed
In recent clinical trials , the \"quality\ " of warfarin management has been characterized by the time in therapeutic range ( TTR ) -- with the therapeutic range being an INR between 2.0 and 3.0 . - pubmed
Resolution of acute inflammation is governed , in part , by lipid mediator class switching from proinflammatory eicosanoids to specialized proresolving mediators , including a recently identified new pathway of mediators , termed maresin conjugates in tissue regeneration ( MCTR ) , which includes MCTR1 , MCTR2 , and MCTR3 . - pubmed
Here , we addressed whether each MCTR can impact the known vascular actions of cysteinyl leukotrienes . - pubmed
Sirt5 + cells in wild - type Kras CRCs are resistant to either chemotherapeutic agents or cetuximab and serve as a reservoir for recurrence . - pubmed
Sirt5 demalonylates and inactivates succinate dehydrogenase complex subunit A ( SDHA ) , leading to an accumulation of the oncometabolite succinate . - pubmed
Succinate binds to and activates a reactive oxygen species - scavenging enzyme , thioredoxin reductase 2 ( TrxR2 ) , to confer chemotherapy resistance . - pubmed
In contrast , Sirt5 + cells exhibit an elevated succinate - to - aKG ratio that inhibits aKG - dependent dioxygenases to maintain cetuximab resistance . - pubmed
CCK - receptor transcripts were also detected and Ca2 + imaging showed that CCK stimulation increased [ Ca2 +]cyt in a dose - responsive manner that was sensitive to CRAC - channel inhibitors . - pubmed
These results provide the first evidence of a potential regulatory system for SOCE in enamel cells and so strengthen the Ca2 + transcytosis paradigm for ER - based transport of bulk Ca2 + . - pubmed
Our findings also implicate enamel cells as a new physiological target of CCK and raise the possibility of an auto / paracrine system for regulating Ca2 + transport . - pubmed
Ca2 + - sensing receptor ( CaSR ) represents a potential therapeutic target for inflammatory bowel diseases and strongly prefers aromatic amino acid ligands . - pubmed
We investigated the regulatory effects of dietary supplementation with aromatic amino acids - tryptophan , phenylalanine and tyrosine ( TPT ) - on the CaSR signalling pathway and intestinal inflammatory response . - pubmed
Dietary supplementation of TPT alleviated histopathological injury and decreased myeloperoxidase activity in intestine challenged with lipopolysaccharide . - pubmed
Supplementation of TPT increased CaSR and phospholipase Cbeta2 protein levels , but decreased inhibitor of NF - kappaB kinase alpha / beta and inhibitor of NF - kappaB ( IkappaB ) protein levels in the lipopolysaccharide - challenged piglets . - pubmed
To conclude , supplementation of aromatic amino acids alleviated intestinal inflammation as mediated through the CaSR signalling pathway . - pubmed
This microfluidic bioanalysis platform was used in conjunction with commercial " mix - detect " assays for glucose , total bile acids , and lactate dehydrogenase ( LDH ) . - pubmed
To highlight this application , hepatocyte spheroids were established in microfluidic devices , injured on - chip by exposure to lipotoxic agent ( palmitate ) , and then connected to the bioanalysis module for daily monitoring of changes in cytotoxicity ( LDH ) , energy metabolism ( glucose ) , and liver function ( total bile acids ) . - pubmed
Microfluidic in - droplet assays revealed increased levels of LDH as well as reduction in bile acid synthesis - results that were consistent with hepatic injury . - pubmed
We have developed a clustering of kinase conformations based on the location of the Phe side chain ( DFGin , DFGout , and DFGinter or intermediate ) and the backbone dihedral angles of the sequence X - D - F , where X is the residue before the DFGmotif , and the DFG - Phe side - chain rotamer , utilizing a density - based clustering algorithm . - pubmed
The beta - propeller phytase ( BPP ) is an enzyme that hydrolyzes phyate to release inorganic phosphorus . - pubmed
Banked bone flaps had the highest overall complication rates ( 51 percent ; p & lt ; 0.001 ) , bone resorption ( 39.7 percent ; p & lt ; 0.001 ) , and failure rates ( 40.2 percent ; p & lt ; 0.001 ) , whereas polyetherether ketone had the highest rates of infection ( 16.1 percent ; p & lt ; 0.001 ) . - pubmed
METHODS : Specimens ( N = 288 ; n = 96 per material ) with a thickness of 1 + - 0.03 mm of the following CAD / CAM materials were fabricated : resin composite ( Lava Ultimate , 3 M ) , polymer - infiltrated ceramic ( VITA Enamic , VITA Zahnfabrik ) , and leucite ceramic ( IPS Empress CAD , Ivoclar Vivadent ) . - pubmed
Resin composite Lava Ultimate showed larger E values compared with the resin hybrid ceramic VITA Enamic and leucite ceramic IPS Empress CAD . - pubmed
Editor 's Note : PrLZ Protects Prostate Cancer Cells from Apoptosis Induced by Androgen Deprivation via the Activation of Stat3 / Bcl-2 Pathway . - pubmed
Hormonal assessment included total testosterone ( TT ) , sex hormone binding globulin ( SHBG ) , free androgen index ( FAI ) , Anti Mullerian Hormone ( AMH ) , 17 - hydroxyprogesterone ( 17 - OHP ) , dehydroepiandrosterone sulfate ( DHEAS ) , follicle stimulating hormone ( FSH ) , luteinizing hormone ( LH ) , thyroid stimulating hormone ( TSH ) and prolactin . - pubmed
VIP and muscarinic synergistic mucin secretion by salivary mucous cells is mediated by enhanced PKC activity via VIP - induced release of an intracellular Ca2 + pool . - pubmed
Mucin secretion by salivary mucous glands is mediated predominantly by parasympathetic acetylcholine activation of cholinergic muscarinic receptors via increased intracellular free calcium ( [ Ca2+]i ) and activation of conventional protein kinase C isozymes ( cPKC ) . - pubmed
VIP - induced secretion is mediated by cAMP - activated protein kinase A ( PKA ) , independently of increased [ Ca2+]i . - pubmed
Synergistic secretion between VIP and the muscarinic agonist , carbachol , was demonstrated but only with submaximal carbachol . - pubmed
Instead , PKA activated by VIP releases Ca2 + from an intracellular pool maintained by the sarco / endoplasmic reticulum Ca2 + - ATPase pump . - pubmed
Calcium release was independent of phospholipase C activity . - pubmed
Synergistic mucin secretion was mimicked by VIP plus either phorbol 12 - myristate 13 - acetate or 0.01 muM thapsigargin , and blocked by the PKC inhibitor , Go6976 . - pubmed
Synergism is therefore driven by VIP - mediated [ Ca2 +]i augmenting cPKC activity to enhance muscarinic mucin secretion . - pubmed
Additional data suggest ryanodine receptors control VIP / PKA - mediated Ca2 + release from a Ca2 + pool also responsive to maximal carbachol . - pubmed
One such etiology found in pregnancy is HL , in which high levels of beta - human chorionic gonadotropin can stimulate production of benign theca lutein cysts , leading to marked hyperandrogenism and virilizing symptoms . - pubmed
Ulinastatin , a urine trypsin inhibitor , relieves systemic inflammation and improves coagulation profiles with however sparse evidence of its effects on blood loss and allogeneic transfusion in this specific population . - pubmed
RESULTS : Compared with placebo , ulinastatin significantly reduced the volume of post - operative blood loss within 24 h ( 688.39 + - 393.55 ml vs 854.33 + - 434.03 ml MD - 165.95 ml , 95%CI - 262.88 ml to - 69.01 ml , p < 0.001 ) and the volume of allogeneic erythrocyte transfusion ( 2.57 + - 3.15 unit vs 3.73 + - 4.21 unit , MD-1.16 unit , 95%CI - 2.06 units to - 0.26 units , p = 0.002 ) . - pubmed
CONCLUSIONS : Ulinastatin reduced post - operative blood loss and allogeneic erythrocyte transfusion in heart surgery with CPB . - pubmed
Bioreducible Phosphonoamidate Pro - drug Inhibitor of Enolase : Proof of Concept Study . - pubmed
We recently described a promising phosphonate inhibitor of enolase for cancers harboring homozygous deletions of ENO1 . - pubmed
We demonstrated that this mutation caused impairment of mitochondrial translation , respiratory deficiency , overproduction of reactive oxygen species ( ROS ) , and decreased mitochondrial membrane potential ( MMP ) , which ultimately led to severe mitochondrial myopathy . - pubmed
Interactions between carbohydrates ( glycans ) and glycan - binding proteins ( GBPs ) regulate a wide variety of important biological processes . - pubmed
The general suppression of nonspecific adducts ( including nonvolatile buffers and salts ) achieved with these tips enables ESI - MS glycan affinity measurements to be performed on C - type lectins , a class of GBPs that bind glycans in a calcium - dependent manner and are important regulators of immune response . - pubmed
What is the best method to remove 2,3 - diphosphoglycerate from hemoglobin ? - pubmed
The gamma - carboxyglutamic acid and epidermal growth factor - like domains of factor X. - pubmed
We have isolated fragments , from tryptic digests of factor X , that consists of the gamma - carboxyglutamic acid ( Gla ) region linked to one or two epidermal growth factor ( EGF)-like domains . - pubmed
The same conclusion was reached when thrombin generation was studied in a system of purified components ( factor Xa , factor Va , prothrombin , phospholipid , and Ca2 + ) . - pubmed
However , experiments in the purified system without phospholipid indicated a direct , albeit weak , interaction between the Gla region of factor Xa and factor Va and between the COOH - terminal EGF - like domain of factor Xa and factor Va. - pubmed
Using domain - specific Fab fragments , we have confirmed that the conformation of the serine protease region alters dramatically upon activation of factor X. - pubmed
Using T7 gene 6 exonuclease , the 3'termini of the fragments were exposed and then hybridized in solution to a biotinylated oligonucleotide complementary to one end of the SUEHGR fragment . - pubmed
Tamoxifen and trifluoroperazine ( Stelazine ) potentiate cytostatic / cytotoxic effects of P-30 protein , a novel protein possessing anti - tumor activity . - pubmed
This reproductive sterility has been attributed to chlordecone 's apparent " estrogenicity " and its ability to inhibit the release of luteinizing hormone ( LH ) from the anterior pituitary . - pubmed
Chlordecone ( 25 - 75 mg / kg ) administration at the time of PMS exposure did not inhibit the ovulatory response to HCG . - pubmed
The specific effect of chloroquine in ' stripping HLA ' from RBC had been assumed to support the concept that HLA class I was adsorbed from plasma . - pubmed
We suggest that the action of chloroquine is to remove beta 2 m alone , which prevents normal class I expression and also results in conformational changes to the class I heavy chain , but that it is not capable of removing the membrane - bound heavy chain . - pubmed
Serum levels of 3 - keto - desogestrel and SHBG during 12 cycles of treatment with 30 micrograms ethinylestradiol and 150 micrograms desogestrel . - pubmed
There was a significant correlation between the SHBG levels and the area under the KDG - concentration - versus - time curves ( AUC ) indicating a pronounced influence of the serum steroid - binding protein upon the pharmacokinetics of KDG . - pubmed
This study , which was carried out as part of an in vitro fertilisation programme ( IVF ) was performed during 162 cycles of which 22 were spontaneous and 140 were stimulated by Clomiphene and HMG . - pubmed
There is a correlation between the levels of progesterone in the blood and the thickness of the endometrium on the 2nd post - laparoscopy day . - pubmed
Ketoconazole , a known cytochrome P-450 inhibitor , inhibited both progesterone ring hydroxylation and side - chain oxidation to steroidal acids . - pubmed
These findings strongly suggest that AM blocks slow inward current inflow via Ca2 + channels in the ventricular muscle . - pubmed
Serum osteocalcin was elevated , but there was little effect on serum alkaline phosphatase , Ca , P , or calcitonin . - pubmed
Pretreatment of animals with 5 - azacytidine results in a different response of tryptophan oxygenase to tryptophan and cortisone administration . - pubmed
Tryptophan affects also the level of thymidine kinase at various stages of liver regeneration . - pubmed
5 - azacytidine given 16 - 40 hours prior to partial hepatectomy resulted in the enhancement of thymidine kinase activity at 24 hours of regeneration . - pubmed
ACTH - secreting medullary carcinoma of the thyroid : monitoring of clinical course with calcitonin and cortisol assays and immunohistochemical studies . - pubmed
This tumor inhibitory factor co - purified with transforming growth factor activity through acid / ethanol extraction and Bio - Gel chromatography . - pubmed
The tumor inhibitory factor is heat stable and requires disulfide bonds for bioactivity . - pubmed
The heat - stable polypeptide ( APF-1 ) required for ATP - dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by DEAE - cellulose . - pubmed
The APF-1 conjugates are stable in sodium dodecyl sulfate / polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid , alkali , heat denaturation , and reduction ; the conjugates are therefore covalent . - pubmed
1 The effects of pretreatment with various inhibitors of anaphylactic mediators on antigen - induced bronchoconstriction were studied in anaesthetized guinea - pigs , actively sensitized according to two different regimens ( one producing IgE- and IgG - like antibodies and the other producing exclusively IgG antibodies).2 The phospholipase A(2)-inhibitors mepacrine and p - bromphenacylbromide caused a dose - dependent inhibition of the antigen - induced bronchoconstriction in guinea - pigs sensitized to produce both IgE and IgG antibodies . - pubmed
In this model , the residual bronchoconstriction evident after combined pretreatment with indomethacin and mepyramine was prevented by additional pretreatment with mepacrine , FPL 55712 or budesonide.5 Arachidonic acid given intravenously caused a marked bronchoconstriction that was prevented by indomethacin but not by budesonide , FPL 55712 or p - bromphenacylbromide.6 Although the same pattern of anaphylactic mediators is released in the two models of anaphylactic bronchoconstriction , a different activation mechanism is indicated by the results obtained with phospholipase A ( 2 ) inhibitors . - pubmed
This contrasts with the primary action of gamma - vinyl - GABA on GABA - transaminase activity . - pubmed
Oxazepam is determined at 254 nm by using a C18 column . - pubmed
In the presence of FBS , calcein leakage was increased with PEG - Chol percentage incorporation and an increase in FBS concentration . - pubmed
Exposure of mesangial cells to platelet - derived growth factor ( PDGF ) BB caused a significant stimulation of cell proliferation and protein synthesis , as measured by [ 3H ] thymidine incorporation and [ 3H ] leucine incorporation respectively . - pubmed
Treatment of mesangial cells with PDGF and angiotensin II rapidly and dose - dependently stimulated mitogen - activated protein ( MAP ) kinase activity , as shown by an assay for activity in vitro using myelin basic protein as a substrate , and by immunoprecipitation of 32P - labelled cells with specific antibodies against the 42 kDa and 44 kDa mitogen - activated protein kinases p42mapk and p44mapk , respectively . - pubmed
Whereas stimulation with PDGF - BB caused a potent and sustained ( for more than 30 min ) phosphorylation and activation of p42mapk and p44mapk , as well as of the upstream activators MAP kinase kinase and c - Raf , the effect of angiotensin II was less potent , reaching a peak at 5 - 10 min and thereafter declining rapidly . - pubmed
In summary , these results suggest that PDGF - BB and angiotensin II differ in their potency and duration of activation of the MAP kinase cascade , which may explain why PDGF - BB is a potent mitogen for mesangial cells , whereas angiotensin II only triggers mesangial - cell hypertrophy . - pubmed
A sustained increase ( P & lt ; 0.05 ) in stroke volume accompanied the prompt and sustained decrease in plasma renin activity and sustained increase ( P & lt ; 0.05 ) in plasma atrial natriuretic peptide during Eu - H2O and Hypo - H2O. - pubmed
We feel that there is now compelling evidence that the GTP - binding proteins play more than just a coordinating role in the actions of both tyrosine kinase and nontyrosine kinase receptor signal transduction . - pubmed
Tumour markers in uraemia : carbohydrate antigen CA 72 - 4 , carcinoembryonic antigen and carbohydrate antigen CA 19 - 9 . - pubmed
Thus , the retinoic acid response mediated by RAR alpha is weak in these cells . - pubmed
Glutamate responses and spontaneous excitatory post - synaptic potentials in DE2 were reversibly blocked in sodium - free saline . - pubmed
Steady - state potentials reached during glutamate superfusion were similar to the reversal potentials for both the spontaneous post - synaptic potentials and glutamate , also suggesting that these potentials may be glutamatergic . - pubmed
Non - N - methyl - D - aspartate receptor antagonists partially blocked spontaneous DE2 excitatory potentials and responses elicited by exogenous glutamate and kainate . - pubmed
The thermal antinociceptive effect induced by morphine administered in the periaqueductal gray was significantly attenuated by neuropeptide FF , whereas that induced by systemically administered morphine was not . - pubmed
The interaction of neuropeptide FF with intracerebrally or systemically administered morphine in a test of tactile allodynia was not significant . - pubmed
This antiallodynic effect is at least partly independent of naloxone - sensitive opioid receptors . - pubmed
Furthermore , neuropeptide FF in the periaqueductal gray attenuates antinociception induced by intracerebrally but not systemically administered morphine . - pubmed
Endocrinological contributions to oncology pertain in particular to the discovery of active substances which inhibit neovascularization ( endostatins ) and some new aspects of the action of antioestrogens and oestrogens . - pubmed
The possible action of hormonal factors in neurodegenerative processes are obvious in ubiquitine , CREB and " heat shock proteins " . - pubmed
Subsequently attention is paid to ryanodine receptors and compounds acting as Ca on the sensor for Ca in the parathyroid bodies and inhibition parathormone secretion . - pubmed
The purpose of this study was to demonstrate the immunological effects on the IgG - antibody repertoire in EAU after topical and systemic allopurinol and steroids . - pubmed
Morphological changes of the small bowel mucosa and activities of daily life ( ADL ) after PEG were also investigated . - pubmed
RESULTS : In the TPN - to - PEG group , the serum level of total protein and albumin , length of duodenal villi , ration of positive proliferative cells , width of duodenal microvilli and ADL markedly increased . - pubmed
In the NG - to - PEG group , body weight and ADL were markedly improved after PEG . - pubmed
Calcium influx through voltage - dependent calcium channels ( VDCCs ) mediates a variety of functions in neurons and other excitable cells , but excessive calcium influx through these channels can contribute to neuronal death in pathological settings . - pubmed
Prolonged exposure to 4HN results in neuronal death , which is prevented by treatment with glutathione and attenuated by the L - type Ca2 + channel blocker nimodipine . - pubmed
Tyrosine phosphorylation of alpha1 VDCC subunits is increased in neurons exposed to 4HN , and studies using inhibitors of tyrosine kinases and phosphatases indicate a requirement for tyrosine phosphorylation in the enhancement of VDCC activity in response to 4HN . - pubmed
At rest , the phosphorus - creatinine indexes were similar in all groups ; pH values at rest did not vary either . - pubmed
Expression of Sox9 in granulosa cells lacking the estrogen receptors , ERalpha and ERbeta . - pubmed
These findings suggest that functional Sertoli cells can transdifferentiate from mature granulosa cells devoid of estrogen receptors as a result of Sox9 expression . - pubmed
Bioassay of Fluometuron for Possible Carcinogenicity ( CAS No . - pubmed
The effect of carotene on the lactic acid production and hexokinase activity of hemolysates from cancerous and noncancerous persons . - pubmed
Both Ero1 - Lalpha and Ero1 - Lbeta interact via disulfide bonds with PDI and support the oxidation of immunoglobulin light chains . - pubmed
In contrast , extracellular HbO2 is highly vasoconstrictive , and the resultant hypertension is a significant side effect of most hemoglobin - based blood substitutes . - pubmed
This interpretation is supported by a strong linear correlation between the magnitude of the blood pressure effect caused by infusion of cross - linked recombinant hemoglobin tetramers in vivo and the rate of NO dioxygenation by these proteins measured in vitro . - pubmed
Calreticulin , a Ca ( 2 + ) chaperone , is found in many different locations in various eukaryotic cells , including lumen of the endoplasmic reticulum , the cell surface , perinuclear areas and cytosolic granules . - pubmed
In parallel with these changes , exendin-4 decreased the cytokine - induced activation of caspase-3 ( by 46 % , p<0.05 ) , and decreased levels of inducible nitric oxide synthase ( by 71 % , p<0.05 ) and reactive oxygen species ( by 27 % , p<0.05 ) . - pubmed
Recently , we proved that tobacco CND41 protease is involved in Rubisco degradation and the translocation of nitrogen during senescence . - pubmed
Using very specific antibodies that were prepared against CND41 - specific peptide ( anti - Val 186 to Ser 206 ) , immunoblot analysis clearly indicated a change in the accumulation and processing of CND41 during the maturation of leaves in whole plants . - pubmed
Further studies of seedlings under senescence induced by combined treatment with nitrogen - starvation and high sucrose confirmed that the processing of CND41 was important for protease activity and senescence . - pubmed
Use of ACTH and prednisolone in infantile spasms : experience from a developing country . - pubmed
BACKGROUND : Adrenocorticotrophic hormone ( ACTH ) and prednisone are both used to treat infantile spasms ( IS ) in West syndrome . - pubmed
ACTH was expensive and difficult to obtain whereas prednisolone was cheap and easily available . - pubmed
The cost of ACTH injection was more than 100 times the cost of oral prednisolone . - pubmed
Although the protein GlmD(Tk ) was predicted as a probable sugar isomerase related to the C - terminal sugar isomerase domain of GlcN6P synthase , the recombinant GlmD(Tk ) clearly exhibited GlcN6P deaminase activity , generating Fru6P and ammonia from GlcN6P. - pubmed
This enzyme also catalyzed the reverse reaction , the ammonia - dependent amination / isomerization of Fru6P to GlcN6P , whereas no GlcN6P synthase activity dependent on glutamine was observed . - pubmed
kodakaraensis cells , glmD(Tk ) was polycistronically transcribed together with upstream genes encoding an ABC transporter and a downstream exo - beta - glucosaminidase gene ( glmA(Tk ) ) within the gene cluster , and their expression was induced by the chitin degradation intermediate , diacetylchitobiose . - pubmed
The results presented here indicate that GlmD(Tk ) is actually a GlcN6P deaminase functioning in the entry of chitin - derived monosaccharides to glycolysis in this hyperthermophile . - pubmed
A comparative study was carried out for the simultaneous determination of 11 pyrethroid insecticides in soil by gas chromatography ( GC)--ion trap ( IT)--mass spectrometry ( MS ) , by means of two different ionization modes ; electron impact and negative chemical ionization and three data acquisition procedures ; full scan , selected ion monitoring and MS / MS . - pubmed
The mutual diffusion coefficients ( D ) of monomeric actin were accurately determined in a G - buffer with a low concentration of KCl from 0 to 10 mM. - pubmed
The Hamaker constant of actin in the Ca ( 2 +)- ATP bound state was determined for the first time as A(H ) = 10.4 + /- 0.6 k(B)T. - pubmed
Tissue total antioxidant capacity ( T - AOC ) , superoxide dismutase ( SOD ) , and thiobarbituric acid reaction substance ( TBARS ) were determined on the 2nd , 4th , and 6th day . - pubmed
BACKGROUND : Intermittent dosing of calcitriol for secondary hyperparathyroidism ( SHPT ) has been associated with greater parathyroid hormone ( PTH ) reduction with fewer calcemic and phosphatemic effects than daily ( QD ) dosing . - pubmed
These cells had an increased sensitivity to IGF-1 and to cyclic AMP , which required phosphatidylinositol-3 ( PI3 )- kinase and the mitogen - activated protein kinase , extracellular signal - regulated protein kinase ( ERK ) for enhanced growth . - pubmed
METHODS : In the present study , the association between ARHI and polymorphisms in genes that contribute to the defence against reactive oxygen species , including GSTT1 , GSTM1 and NAT2 , was tested . - pubmed
Such action is mediated by interaction of androgen receptor ( AR ) promoter with nuclear proteins , which are involved in transcriptional regulation of androgen responsive genes . - pubmed
The cyclin - dependent kinase inhibitor p27 ( Kip1 ) arrests cell cycle progression through G1 / S phases and is regulated by phosphorylation of serine / threonine residues . - pubmed
Recently , we identified the serine / threonine kinase , KIS , which phosphorylates p27 ( Kip1 ) on serine 10 leading to nuclear export of p27 ( Kip1 ) and protein degradation . - pubmed
Signaling by the peptide ligand apelin and its cognate G protein - coupled receptor APJ has a potent inotropic effect on cardiac contractility and modulates systemic vascular resistance through nitric oxide - dependent signaling . - pubmed
In addition , there is evidence for counterregulation of the angiotensin and vasopressin pathways . - pubmed
The QSAR is an alternative method for the research of new and better Vitamin D analogues with affinity for the VDR receptor . - pubmed
This paper describes the results of applying the Radial Distribution Function ( RDF descriptors ) approach for predicting the VDR affinity of 38 vitamin D analogues . - pubmed
CONCLUSION : Repeated determination of the BASDAI and BASFI by the patients ( using paper forms or personal digital assessments ) may help to identify candidates for treatment intensification , to optimize infliximab injection regimens in good responders , and to avoid unnecessary switching from one TNFalpha antagonist to another . - pubmed
Theoretical studies on the thermochemistry of stable closed - shell C1 and C2 brominated hydrocarbons . - pubmed
The enthalpies of formation of stable closed shell C1 and C2 brominated hydrocarbons have been predicted using Gaussian-3X model chemistry . - pubmed
124 , 064106 ( 2006 ) ] , which requires only a small number of fast nonequilibrium MD simulations of the system in both forward and time reversed directions along the RC , we reconstruct the PMF : ( 1 ) of deca - alanine as a function of its end - to - end distance , and ( 2 ) that guides the motion of potassium ions through the gramicidin A channel . - pubmed
Serine endopeptidases , cutinases , acetylcholinesterases , cellulases , among other enzymes , contain these triads . - pubmed
The optimisation of the most important operating conditions affecting lipase - catalysed transesterification and the yield of alkyl esters , such as the type and form of lipase , the type of alcohol , the presence of organic solvents , the content of water in the oil , temperature and the presence of glycerol , are discussed . - pubmed
hERG1 is a member of the cyclic nucleotide binding domain family of K (+ ) channels . - pubmed
Alignment of cyclic nucleotide binding domain channels revealed an evolutionary conserved sequence HwX(A / G)C in the S5 domain . - pubmed
We reasoned that histidine 562 in hERG1 could play an important structure - function role . - pubmed
To explore this role , we created in silica models of the hERG1 pore domain based on the KvAP crystal structure with Rosetta - membrane modeling and molecular - dynamics simulations . - pubmed
Simulations indicate that the H562 residue in the S5 helix spans the gap between the S5 helix and the pore helix , stabilizing the pore domain , and that mutation at the H562 residue leads to a disruption of the hydrogen bonding to T618 and S621 , resulting in distortion of the selectivity filter . - pubmed
In conclusion , interactions between H562 in the S5 helix and amino acids in the pore helix are important determinants of hERG1 potassium channel function , as confirmed by theory and experiment . - pubmed
Other PFCs such as perfluorohexanesulfonate ( PFHxS ) , perfluorooctanoic acid ( PFOA ) , and acids with chain lengths up to C11 were also detected , with mean levels ranging between 0.50 and 1.45 ng / g wet weight . - pubmed
Accumulation in liver is suggested for PFOS and the perfluorocarboxylic acids with carbon chain lengths C9 , C10 , and C11 . - pubmed
Newer promising strategies along with recent patented inventions may also include thiazolidinediones , folates , tetrahydrobiopterin , cyclopentone prostaglandins and aldose reductase inhibitors with well known effects on vascular redox , but with still unclear results on clinical outcome . - pubmed
In experimentally exploring the adaptive landscape of the evolution of resistance to beta - lactam antibiotics in enteric bacteria , we noted that a regulatory mutation that increases beta - lactamase expression by about 2 - fold has a very strong tendency to be fixed at or near the end of the evolutionary pathway . - pubmed
To understand the basis of the difference , we carried out experiments in which the expression of beta - lactamase was under the control of a tunable arabinose promoter . - pubmed
Best medical treatment is defined as optimal drug therapy for control of the major risk factors ( blood pressure & lt ; or = 125/80 mmHg , LDL cholesterol & lt ; or = 100 mg / dL , HbA1c & lt ; or = 6.5 % ) . - pubmed
A theoretical study on binding orientations and quantitative structure - activity relationship of thiophene derivatives as inhibitors towards tubulin has been carried out by using the docking analysis and the comparative molecular field analysis . - pubmed
The conjugation reaction was effected by squaric acid chemistry and monitored in virtually real time by SELDI - TOF MS . - pubmed
In samples stimulated with 0.3 microm ADP , fibrinogen binding decreased from 41 + /- 4 % to 31 + /- 3 % ( P & lt ; 0.01 , n = 11 ) after acetylcholine administration . - pubmed
The vasodilator response to acetylcholine was significantly impaired during infusions of ET-1 and l - NMMA . - pubmed
ET-1 did not affect platelet activity per se , whereas l - NMMA increased platelet P - selectin expression . - pubmed
Both ET-1 and l - NMMA attenuated the acetylcholine - induced inhibition of platelet activity . - pubmed
CONCLUSIONS : Our study indicates that , further to inhibiting endothelium - dependent vasodilatation , ET-1 may also attenuate endothelium - dependent inhibition of platelet activation induced by acetylcholine . - pubmed
The novel roles of glucagon - like peptide-1 , angiotensin II , and vitamin D in islet function . - pubmed
As such , the islets perform critical biological activities in synthesizing metabolic peptide hormones , notably insulin and regulating body glucose homeostasis . - pubmed
Of great interest in this context , glucagon - like peptide-1 can improve glycemic control by regulating insulin secretion and islet cell mass ; vitamin D can regulate islet physiology directly by binding its receptors ; in addition , the peptide hormone angiotensin II has been implicated in islet function and exhibits effects on islet cell secretion as well as cell mass . - pubmed
Microbial respiration was positively correlated with the degradation rates of metalaxyl , azoxystrobin and chlorpyrifos , compared to phenoloxidase which showed no correlation . - pubmed
Pazopanib is an orally administered , multi - tyrosine kinase inhibitor that interrupts tumor growth in renal cell carcinoma . - pubmed
We directly compared predominant viral sequences derived from 52 mother - child transmission pairs following vertical infection and identified 1,475 sites of mother - infant amino acid divergence within Nef , Gag , and Pol . - pubmed
Clearances of small solutes like urea are higher than in hemofiltration and of middle solutes like beta ( 2 )- microglobulin are higher than in hf - HD . - pubmed
Glucose and fatty acids synergize to promote B - cell apoptosis through activation of glycogen synthase kinase 3beta independent of JNK activation . - pubmed
Jun NH2 - terminal kinase ( JNK ) activation peaked at the lowest glucose concentration , in contrast to a progressive reduction in IRS2 protein and impairment of insulin receptor substrate signaling . - pubmed
CONCLUSIONS / SIGNIFICANCE : Glucose in the presence of FFA results in synergistic effects on ER stress , impaired insulin receptor substrate signaling and Gsk3beta activation . - pubmed
The first NMR structure of a Cu ( I )-bound metallochaperone model with the conserved sequence MT / HCXXC revealed that at pH ~3.0 and ~6.8 Cu ( I ) binds through one Cys and the Met rather than the two Cys residues , differently than at pH ~8.5 . - pubmed
Many of them rely on the Hsp90 chaperone system for full function and are further fine - tuned by Hsp90 - associated peptidyl - prolyl isomerases such as FK506 - binding proteins 51 and 52 . - pubmed
The peptidyl - prolyl isomerase domain of FKBP51 contains a high - affinity binding site for the natural products FK506 and rapamycin and has further been shown to convey most of the inhibitory activity on the glucocorticoid receptor . - pubmed
Here , a series of high - resolution structures of the peptidyl - prolyl isomerase domain of FKBP51 as well as a cocrystal structure with the prototypic ligand FK506 are described . - pubmed
The results obtained using the computational methods gave valuable information about the probable metabolites of ( 1 ) ( qualitatively ) and also about the important interactions of this lead compound with the amino acid residues of the active site of CYP2C9 . - pubmed
RESULTS : Of the 10 AVS criteria identified in the literature , one criterion ( ACTH stimulation , positioning : cortisol [ adrenal]/cortisol [ periphery ] [ Ca / Cp ] & gt ; 5.0 and lateralization : aldosterone / cortisol [ A / C ] [ dominant { D } ] : A / C [ nondominant { ND } ] & gt ; 4:1 ) was the most accurate in identifying and correctly predicting lateralization of disease ( P value range : & lt ; 0.001 - 0.0369 ) . - pubmed
The second most accurate criterion was no ACTH stimulation , positioning Ca / Cp & gt ; 1.1 and lateralization : A / C ( D ): A / C ( ND ) & gt ; 2:1 . - pubmed
The Ca ( 2 + ) /calmodulin ( CaM ) signaling pathway mediates the heat stress ( HS ) response and acquisition of thermotolerance in plants . - pubmed
Ca ( 2 + ) and A23187 treatments under HS generated an intense and sustained increase in [ Ca ( 2 + ) ] cyt and accelerated the expression of CaM1 - 1 and sHSPC / N genes , which suggests that HS - induced apoplastic Ca ( 2 + ) influx was responsible for the [ Ca ( 2 + ) ] cyt transient and downstream HS signaling . - pubmed
The chromatographic separation was carried out on a Hypersil BDS C18 column , and gradient elution was employed with a mobile phase containing acetonitrile and water . - pubmed
CYP1A1 and CYP1B1 - mediated biotransformation of the antitrypanosomal methamidoxime prodrug DB844 forms novel metabolites through intramolecular rearrangement . - pubmed
In the current study , we compared the metabolism of DB844 by hepatic and extrahepatic cytochrome P450s to determine whether differences in metabolite formation underlie the observed GI toxicity . - pubmed
In addition , MY was confirmed with a synthetic standard and detection of nitric oxide ( NO ) release when DB844 was incubated with CYP1A1 . - pubmed
dabigatran ) and factor Xa inhibitors ( e.g. - pubmed
In contrast , treadmill exercise and MPH alleviated the ADHD - induced the decrease of balance and the number of calbindine - positive cells , and the increase of GFAP expression and Bax / Bcl-2 ratio in the cerebellum ( P< ; 0.05 , respectively ) . - pubmed
Androgens , 17 - beta - estradiol ( 17 beta -E2 ) , and insulin levels were measured in plasma and/or serum and leptin and visfatin levels were assayed in both serum and follicular fluid ( FF - leptin , FF - visfatin ) . - pubmed
Previously , we have shown that intermittent parathyroid hormone ( PTH ) bone anabolic therapy involves SOST expression reduction by inhibiting myocyte enhancer factor 2 ( MEF2 ) , which activates a distant bone enhancer . - pubmed
PTH - induced Sost suppression was associated with specific rapid nuclear accumulation of HDAC5 and co - localization with MEF2s in nuclear speckles requiring serine residues 259 and 498 , whose phosphorylations control nucleocytoplasmic shuttling . - pubmed
Curcumin protects renal tubular epithelial cells from high glucose - induced epithelial - to - mesenchymal transition through Nrf2 - mediated upregulation of heme oxygenase-1 . - pubmed
This process may occur through abrogation of high glucose ( HG)-induced oxidative stress via activation of nuclear factor ( erythroid - derived 2 )- like 2 ( Nrf2 ) and heme oxygenase-1 ( HO-1 ) in kidney tubular epithelial cells . - pubmed
The present study revealed that curcumin was able to prevent events associated with EMT , including the downregulation of E - cadherin and the increased expression of alpha - smooth muscle actin . - pubmed
Notably , knockdown of Nrf2 with small interfering RNA prevented the curcumin - induced elevation in expression of HO-1 and the associated anti - fibrotic effects . - pubmed
Clinical Research on Albumin - Bound Paclitaxel - Based Chemotherapy for Advanced Esophageal Cancer . - pubmed
Both PBF and PAbdF were associated with HDL and LDL , but have opposite correlation with triglyceride . - pubmed
Men with GOb had lower levels of percent lean mass ( PLM ) , testosterone and bioavailable testosterone , and higher insulin and glucose levels . - pubmed
Men with AbO had lower arm and leg fat , higher insulin levels and triglycerides . - pubmed
RESULTS : KfoC has broad donor substrate specificity and acceptor promiscuity , making it an attractive tool enzyme for use in structurally - defined chimeric glycosaminoglycan oligosaccharide synthesis in vitro . - pubmed
CONCLUSION AND GENERAL SIGNIFICANCE : These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan oligosaccharides and designing future strategies for directed evolution of KfoC in order to create mutants toward user - defined goals . - pubmed
We demonstrate the immobilization of formate , formaldehyde , and alcohol dehydrogenases on one - and - the - same electrode for direct CO2 reduction . - pubmed
This method of immobilization of enzymes on electrodes offers the opportunity for electrochemical application of enzymatic electrodes to many reactions in which a substitution of the expensive sacrificial co - enzyme NADH is desired . - pubmed
Isoprene Side - chain of SMTP is Essential for Soluble Epoxide Hydrolase Inhibition and Cellular Localization . - pubmed
Here , we investigate the yet - to - be - characterized function of the isoprene side- chain of SMTPs in sEH inhibition and cellular distribution . - pubmed
The introduction of a terminal hydroxy group exceptionally unaffected epoxide hydrolase , but led to impaired cellular localization , resulting in diminution of cellular epoxide hydrolase inhibition . - pubmed
Thus , the isoprene side - chain of SMTP is an important pharmacophore for epoxide hydrolase inhibition and cellular localization . - pubmed
Women receiving mifepristone and misoprostol could choose office follow - up with an ultrasound evaluation one to two weeks after mifepristone or telephone follow - up with a scheduled telephone interview at one week post abortion and a second telephone call at four weeks to review the results of a home urine pregnancy test . - pubmed
Soluble Receptors for Tumor Necrosis Factor and Neopterin as Parameters of Cell - Mediated Immune Activation . - pubmed
In cancer and autoimmune diseases , measurement of sTNF - Rs and neopterin provides useful information for monitoring . - pubmed
The quantification of sTNF - Rs and neopterin concentrations often provide even superior information than that obtained with classical disease markers , probably due to the direct involvement of the " interferon - gamma / tumor necrosis factor - alpha system " in the pathogenetic mechanisms of the diseases . - pubmed
Data imply that measurement of sTNF - Rs , especially of the 75kD - type , is a useful adjunct for quantification of the Th-1 type immune response , sharing many characteristics with neopterin . - pubmed
MicroRNA-21 drives severe , steroid - insensitive experimental asthma by amplifying phosphoinositide 3 - kinase - mediated suppression of histone deacetylase 2 . - pubmed
Treatment with Ant-21 , or the pan - PI3 K inhibitor LY294002 , reduced PI3 K activity and restored HDAC2 levels . - pubmed
BACKGROUND : Alkaptonuria ( AKU ) is an ultra - rare inborn error of metabolism characterized by homogentisic acid ( HGA ) accumulation due to a deficient activity of the homogentisate 1.2 - dioxygenase ( HGD ) enzyme . - pubmed
METHODS : Gel electrophoresis , Western Blot , Congo Red - based assays and electron microscopy were used to investigate the effects of HGA on the aggregation and fibrillation propensity of amyloidogenic proteins and peptides [ Abeta ( 1 - 42 ) , transthyretin , atrial natriuretic peptide , alpha - synuclein and SAA ] . - pubmed
LC / MS and in silico analyses were undertaken to identify possible binding sites for HGA ( or its oxidative metabolite , a benzoquinone acetate or BQA ) in SAA . - pubmed
RESULTS : We found that HGA might act as an amyloid aggregation enhancer in vitro for all the tested proteins and peptides in a time- and dose- dependent fashion , and identified a small crevice at the interface between two HGD subunits as a candidate binding site for HGA / BQA . - pubmed
Influence of HDL particles on cell - cholesterol efflux under various pathological conditions . - pubmed
In controls , there were strong positive correlations between ABCA1 - dependent cholesterol efflux and small prebeta-1 concentrations ( R2 = 0.317 ) and SR - BI - dependent cholesterol efflux and large ( alpha-1 + alpha-2 ) HDL particle concentrations ( R2 = 0.774 ) . - pubmed
HDL anti - oxidative capacity was negatively influenced by high plasma beta - sitosterol levels , but not by the concentrations of HDL particles , TG , SAA , fibrinogen , or MPO . - pubmed
BIIB021 : A novel inhibitor to heat shock protein 90 - addicted oncology . - pubmed
BIIB021 is an orally available , fully synthetic novel small - molecule heat shock protein 90 inhibitor that has shown strong antitumor activities in a large number of preclinical models and is now under clinical investigation . - pubmed
Body composition , physical fitness ( 1,200 running , sit - ups , back strength , sit and reach , side - steps ) and blood variables ( low - density lipoprotein cholesterol , glucose , reactive oxygen species , and malondialdehyde ) were examined before and after 16 weeks of training . - pubmed
The rationale is based on the assumption that DMSO supplementation should induce long - term negative effects on both pre- and post - implantation embryo development . - pubmed
Methods : DMSO induced oxidative stress , ER stress , autophagy , mitophagy , signaling responsible genes and proteins were determined by RT - qPCR , Western blotting , immunofluorescence , and confocal microscopy . - pubmed
Notably , DMSO - treated 2 - cell embryos showed increased expression of unfolded protein response genes such as Hspa5 , Hsp90b1 , Ddit3 , Atf4 , and Xbp1 . - pubmed
Further , DMSO - induced endoplasmic reticulum stress increased mitochondrial Ca2 + levels , induced mitochondrial depolarization / dysfunction , and induced apoptotic cell death via the JNK / ATF2 - dependent pathway . - pubmed
Consequently , treatment with DMSO increased the expression of autophagy initiation- , phagophore elongation- , and autophagosome formation - related genes , as well as localization of PINK1 / Parkin , which are the main mediators of mitophagy , in mitochondria . - pubmed
Finally , exogenous lactate administration can significantly attenuate cell death in neuronal cultures following exposure to lethal OGD . - pubmed
l - NAME administration led to increased blood pressure ( BP ) ( 30 % ) , decreased nitric oxide synthase ( NOS ) activity ( 50 % ) , and increases in NADPH oxidase ( NOX)-dependent superoxide anion production ( 60 % ) and p47phox protein level ( 65 % ) . - pubmed
The co - administration of quercetin prevented the increase in BP and the activation of NOX but did not modify the decrease in NOS activity caused by l - NAME . - pubmed
In addition , quercetin affected oxidative stress parameters as glutathione oxidation , and the activities of oxidant detoxifying enzymes superoxide dismutase , glutathione peroxidase , and catalase . - pubmed
Thus , quercetin administration counteracts l - NAME effects on NO bioavailability determinants in vivo , essentially through controlling NOX - mediated superoxide anion production . - pubmed
This study uses an in vitro system , consisting of monoculture and direct co - cultures of monocytes and MSC on screw - shaped machined and oxidized titanium implants in combination with scanning electron microscopy , enzyme - linked immunosorbent assay , flow cytometry , cell sorting , and quantitative polymerase chain reaction . - pubmed
Functional roles of tyrosine 185 during the bacteriorhodopsin photocycle as revealed by in situ spectroscopic studies . - pubmed
Tyrosine 185 ( Y185 ) , one of the aromatic residues within the retinal ( Ret ) chromophore binding pocket in helix F of bacteriorhodopsin ( bR ) , is highly conserved among the microbial rhodopsin family proteins . - pubmed
In the state - of - the - art red QLED , an ultrathin insulating layer inserted between the QD layer and the oxide electron - transporting layer ( ETL ) is crucial for both optimizing charge balance and preserving the QDs ' emissive properties . - pubmed
Suppressing exciton quenching at the ETL - QD interface , which is identified as being obligatory for high - performance devices , is achieved by adopting Zn0.9 Mg0.1 O nanocrystals , instead of ZnO nanocrystals , as ETLs . - pubmed
Optimizing charge balance is readily addressed by other device engineering approaches , such as controlling the oxide ETL / cathode interface and adjusting the thickness of the oxide ETL . - pubmed
Results : Hesperidin improved self - renewal ability and chondrogenesis of MSCs , inhibited secretion of pro - inflammatory cytokines IFN - gamma , IL-2 , IL-4 and IL-10 , and suppressed the expression of p65 . - pubmed
Overexpression of p65 was able to reverse the hesperidin inhibited secretions of pro - inflammatory cytokines , and abolish the enhancing effect of hesperidin on chondrogenesis of MSCs . - pubmed
In this study , the OmniChange multi - site mutagenesis method was applied to reengineer the P450 BM3 substrate specificity towards the hydroxylation of CTAB by simultaneous mutagenesis of four previously reported positions ( R47 , Y51 , F87 , and L188 ) . - pubmed
1740 clones from the P450 BM3 OmniChange library were screened with the NADPH depletion assay . - pubmed
A total of 696 clones were rescreened with the NADPH depletion and an Ampliflu Red/ horseradish peroxidase based H2 O2 detection assay . - pubmed
Several improved P450 BM3 variants were identified and finally four were kinetically characterized with respect to CTAB hydroxylation , based on both performance and coupling efficiency . - pubmed
Based on NADPH consumption , the P450 BM3 variant P3A8 ( R47E / Y51M / F87V / L188E ) displayed an initial activity ( 64.9 + -4.8 s-1 , 13.5 - fold increased activity compared with wild - type P450 BM3 ) , which nearly matches the specific activity for its natural fatty acid substrate ( palmitic acid ( 32 - 122 s-1 ) ) . - pubmed
HPLC - MS / MS detection confirmed that P3A8 and P2E7 ( R47D / Y51L / F87V / L188A ) form 13 and 35 times more 2 - hydroxylated CTAB than P450 BM3 . - pubmed
In addition , di - hydroxylated CTAB products were detected for all four investigated P450 BM3 variants ( up to a yield of 77 % ; P3A8 ) . - pubmed
The present study assessed the effect of Contingency Management ( CM ) combined with Cognitive - Behavioral Treatment ( CBT ) on certain in - treatment behaviors ( treatment retention , in - treatment smoking abstinence , and weekly decrease of cotinine levels ) and examined the effects of these in - treatment behaviors on smoking status at a 6 - month follow - up . - pubmed
A CT scan revealed an enhanced hypertrophied wall of the short segment of the small intestine , and 99 m Tc - labeled human serum albumin scintigraphy revealed protein leakage into the thickened wall of the small intestine . - pubmed
CIH also resulted in hippocampus neuron apoptosis with increased caspase 3 level , dUIP nick end labeling ( TUNEL)-positive neurons number and cognitive impairment verified by prolonged latency and shortened time in the target quadrant in Morris water maze test . - pubmed
However , pretreatment of NF - kappaB inhibitor BAY 11 - 7082 inhibited the activation of NF - kappaB under CIH condition and also significantly reduced the phosphorylation of JNK as well as c - Jun , ATF2 , and JunD. - pubmed
AlkB monooxygenases in bacteria are responsible for the hydroxylation of medium- and long - chain n - alkanes . - pubmed
Hexadecyl CoA and octadecyl CoA could effectively release the CrgA binding and start the transcription of alkB2 gene , implying a positive regulation of metabolic intermediate . - pubmed
18F - Fludeoxyglucose PET / CT in SCLC : Analysis of the CONVERT Randomized Controlled Trial . - pubmed
INTRODUCTION : We used phase-3 CONVERT trial data to investigate the impact of fludeoxyglucose F 18 ( 18F - FDG ) positron emission tomography ( PET)/computed tomography ( CT ) in SCLC . - pubmed
However , this analysis can not support the use or omission of 18F - FDG PET / CT owing to study limitations . - pubmed
Novel btk inhibitors such as acalabrutinib , zanubrutinib , and tirabrutinib are designed to improve on the safety and efficacy of first - generation btk inhibitors such as ibrutinib . - pubmed
Data from single - arm clinical trials suggest that , compared with ibrutinib , second - generation btk inhibitors have comparable efficacy and might have a more favourable toxicity profile . - pubmed
Heat shock protein 70 ( HSP70 ) is an ATP - dependent molecular chaperone , and it has been shown that its levels increase after exposure to various types of stress , including ischemia . - pubmed
In addition , Tat - HSP70 treatment significantly reduced lipid peroxidation and increased Cu , Zn - superoxide dismutase activities in the spinal cord , but glutathione peroxidase and Mn - superoxide dismutase activities remained unchanged . - pubmed
Salicylic acid ( SA ) mediated defence response was up - regulated in symptomless roots , compared with root gall tissue . - pubmed
The PbBSMT derived Methyl - SA potentially leads to increased pathogen tolerance in uninfected roots . - pubmed
When cultivated on M1 , all isolates produced EPS exhibiting almost the same monosaccharide profile with mannose , glucose and arabinose being the main monomers . - pubmed
Various characterization techniques would demonstrate the successful preparation of resultant CNC - PhE - PEGMA with uniform nanoscale size , remarkable fluorescent properties and extremely low cytotoxicity . - pubmed
In this work , the interfacial friction angle of the polymer pellets in the partially plasticized samples of polymethyl methacrylate ( PMMA ) , polypropylene ( PP ) , and nylon66 ( PA66 ) were characterized by a super - high magnification lens zoom 3D microscope . - pubmed
While allosensitization can occur when a patient comes in contact with foreign antigens , this problem appears more common in female recipients of haploidentical stem cell transplants , due to the development of anti - HLA antibodies during pregnancy against offspring 's HLA antigens . - pubmed
The incidence of DSAs in haploidentical transplants is approximately 15 - 20%;1 however , can be as high as 42.9 % in parous females.2 Until now , only allosensitization of the recipient against donor HLA antigens has been studied . - pubmed
Nevertheless , multiparous female donors are not uncommon in haploidentical transplant setting , and may carry a similar risk of developing anti - HLA antibodies against recipient 's HLA antigens on the unshared haplotype . - pubmed
To investigate if these genes are involved in defense against the macrocyclic lactone insecticide ivermectin in this insect , each gene was silenced using RNA interference or MDR protein activity was inhibited using a chemical inhibitor , verapamil , before challenging the insects with a dose of ivermectin . - pubmed
BACKGROUND : Tislelizumab is an investigational , humanized , IgG4 monoclonal antibody with high affinity and binding specificity for programmed cell death-1 ( PD-1 ) that was engineered to minimize binding to FcgammaR on macrophages in order to abrogate antibody - dependent phagocytosis , a mechanism of T - cell clearance and potential resistance to anti - PD-1 therapy . - pubmed
Relaxed hair was treated with different MW peptides derived from keratin protein and an amino acid , L - Leucine . - pubmed
Iso - C15 : 0 , C15 : 0 anteiso , and summed feature 3 ( C16 : 1 omega6c and/or C16 : 1 omega7c ) were found to be the primary cellular fatty acids in strain C33T. - pubmed
Male residents with low education levels suffer from larger effects of PM1 and SO2 on the incidence rate of lung cancer . - pubmed
Both gain- and loss - of - function studies , including CCK-8 , EdU assay , transwell experiment and wound - healing assay were used to investigate the role of ANXA1 in PTC progression . - pubmed
Besides , the combination of cobicistat - abacavir - rilpivirine HIV drugs demonstrated the highest in silico efficacy of inhibiting SARS - CoV-2 spike glycoprotein . - pubmed
In order to establish standardized MS - based screening assays , we studied 28 sequences with documented NMR structures in ( usually ~100 mM ) potassium , and report here their circular dichroism ( CD ) , melting temperature ( Tm ) , NMR spectra and electrospray mass spectra in 1 mM KCl/100 mM trimethylammonium acetate . - pubmed
In group I the basal body temperature ( BBT ) ( P less than 0.001 ) and the plasma progesterone concentrations ( P less than 0.05 ) were significantly elevated on the 1st day following the luteinizing hormone ( LH ) peak . - pubmed
In group II , THE BBT was slightly elevated on the 1st day following the LH peak ( P less than 0.05 ) , but the increase in the plasma progesterone concentrations occurred only on the 2nd day following the LH peak . - pubmed
A method for the determination of estradiol metabolic clearance rate ( MCR ) and production rate ( PR ) in blood , using radioactive isotope dilution techniques , has been described . - pubmed
Hemodynamic profiles , arterial blood gases , plasma glucose , and serum amylase were obtained before laparotomy , at bile injection , and at hourly intervals . - pubmed
C8H10N2O3 , Mr = 182.18 , monoclinic , P2(1 ) , a = 6.6405 ( 7 ) , b = 7.9493 ( 9 ) , c = 8.3662 ( 9 ) A , beta = 103.07 ( 1 ) degrees , V = 430.18 ( 8) A3 , Z = 2 , Dx = 1.41 Mg m-3 , lambda ( Cu K alpha ) = 1.54184 A , mu = 0.879 mm-1 , F(000 ) = 192 , T = 298 K , R = 0.037 for 1247 reflections with Fo greater than or equal to 4 sigma ( Fo ) . - pubmed
The configuration at C7 is S. - pubmed
Under LD 12:12 and reversed LD 12:12 cycles , superfused pineal glands showed a rhythmic melatonin secretion : Scotophase was associated with high titers and photophase with low titers . - pubmed
However , the forward transmission of Nd - YAG energy through the sapphires varied ( SMTR , 85 % ; MTR , 83 % ; MTRL , 75 % ; OS , 54 % ; LT , 69 % ) . - pubmed
Substance P ( 1 microM ) reduced basal secretion of SLI in agreement with earlier studies . - pubmed
Addition of pyrilamine during substance P perfusion increased IRG secretion whereas addition of cimetidine resulted in a delayed decrease on removal of both compounds . - pubmed
Histamine ( 1 and 10 microM ) increased SLI secretion and reduced IRG secretion . - pubmed
Pyrilamine increased and cimetidine decreased IRG secretion but neither drug influenced SLI secretion . - pubmed
Pyrilamine had no effect on histamine - stimulated SLI secretion but inhibition of IRG secretion by histamine was converted to stimulation . - pubmed
Cimetidine potentiated histamine stimulation of SLI secretion and inhibition of IRG secretion . - pubmed
IN CONCLUSION : ( 1 ) substance P inhibition of SLI secretion is unlikely to be mediated via release of histamine . - pubmed
( 3 ) Histamine weakly stimulates SLI secretion but there may be both inhibitory and stimulatory pathways acting via H2 - and H1 - receptors , respectively . - pubmed
These data show that intracellular methicillin selection facilitates isolation of mutations in genes required for intracellular growth and strengthens the premise that listeriolysin O is essential for intracellular growth . - pubmed
Peak A did not correspond to either bradykinin or other known kinins , such as lysyl - bradykinin and T - kinin , whereas peak B corresponded to bradykinin . - pubmed
After hydrophobic chromatography on Octyl Sepharose CL 6B , separation from other contaminating maltases was achieved by gel filtration on Ultrogel ACA 22 . - pubmed
Transient induction of single GST - P positive hepatocytes by DEN . - pubmed
Neither acyclovir , FIAU , nor the herpes thymidine kinase alone is harmful to cells . - pubmed
The urinary gamma - glutamyl transpeptidase - to - creatinine ratio in spot samples was simple and quick to measure and was correlated with the 24 - hour enzyme excretion . - pubmed
Lubrol PX inhibited enzyme activity , and phospholipid reversed this inhibition . - pubmed
Kinetic and spectroscopic data on the reaction of selenite radicals with some proteins and parallel inactivation studies generally reflect knowledge on the amino acid residues mainly involved in the radical attack . - pubmed
The investigations at different pH values on the reactivity of selenite radicals with amino acids and proteins and on the transient spectra of the reaction products exhibit different behaviour for the various acid - base structures of the selenite radicals , reflecting the influence of particular ionizable groups in the reacting molecules and the structure modifications at the level of proteins . - pubmed
Characterization and mechanism of formation of reactive products formed during peroxidase - catalyzed oxidation of p - phenetidine . - pubmed
OFF products of horseradish peroxidase ( EC 1.11.1.7)-catalyzed oxidation of p - phenetidine were isolated and reactive species were trapped with reduced glutathione ( GSH ) and butylated hydroxyanisole ( BHA ) . - pubmed
Butorphanol , an exogenous opiate which preferentially generalizes to the kappa - sigma opiate receptors , is a potent initiator of feeding . - pubmed
In these studies , we examined the effect of peripherally administered putative satiety substances , cholecystokininoctapeptide , somatostatin , bombesin , gastrin - releasing peptide , thyrotropin - releasing hormone , calcitonin and glucagon on butorphanol induced feeding . - pubmed
This effect is mimicked by the incubation of normal fibroblasts with dehydroepiandrosterone , a strong inhibitor of glucose-6 - phosphate dehydrogenase . - pubmed
Acetylcholinesterase is functional at tonic fibre endplates , but the absence of the collagen - tailed form may account for the lower efficiency of the enzymic removal of acetylcholine there . - pubmed
Our approach was to combine desmin staining with identification of vitamin A ( autofluorescence ) , lipid droplets ( Sudan III ) , vimentin , laminin and tenascin , using cryostat sections : Immunofluorescence , double - immunofluorescence or immunoperoxidase techniques were used . - pubmed
The activation of porcine pancreatic lipase by cis - unsaturated fatty acids . - pubmed
The overall angle of the fatty acid , the position of its double bond and the presence of a carboxyl group were critical factors in whether the fatty acid effectively increased lipase activity . - pubmed
When the substrate is emulsified by taurodeoxycholate , the pH optimum for lipase ranges from 6.2 to 7.0 . - pubmed
In the presence of cis - unsaturated fatty acids , the overall activity of lipase increases , the pH optimum shifts , and the pH - activity curve becomes biphasic , with one optimum around pH 7.7 , and the other around pH 8.8 . - pubmed
Fluorescence studies indicate that fatty acids bind near aromatic residues in lipase , particularly tryptophan . - pubmed
10(-4 ) M. The far - UV CD data also show that cis - unsaturated fatty acids change the conformation of lipase . - pubmed
Because the three - dimensional crystal structure of lipase is known , a model is proposed to describe how cis - unsaturated fatty acids increase lipase activity . - pubmed
Through the release of a variety of relaxing and contracting factors , including nitric oxide and endothelin-1 , the endothelium exerts a complex paracrine influence on vascular smooth muscle cells . - pubmed
column packed with 4 - microns Nova Pack C18 using a mobile phase of water - acetonitrile - triethylamine ( 700:300:4 , v / v / v ) ( adjusted to pH 7.5 with orthophosphoric acid ) . - pubmed
Effect of intense angiotensin II suppression on the diuretic response to furosemide during chronic ACE inhibition . - pubmed
Similarly , urine volumes , in response to furosemide , increased after captopril ( 238 + /- 90 versus 283 + /- 111 mL , P & lt ; .05 , and 245 + /- 78 versus 311 + /- 92 mL , P & lt ; .01 , 1 and 2 hours after dosing ) . - pubmed
Oxidative stress can result in glutathione depletion , lipid peroxidation , membrane damage and DNA strand breaks as well as activation of proteases , nucleases and protein kinases . - pubmed
Analyse hat purified the toxin protein , we find that its amino acid composition was similar with 135 - kDa P1 protein , and its sequence of N - terminal 18 amino acid was same with that of part of the P1 protein . - pubmed
From these data we can suggest that mGlu receptors act inversely to the NMDA receptors in the dorso - lateral periaqueductal gray area and that L - AP-3 is a partial agonist rather than an antagonist of mGlu receptors within the periaqueductal gray area . - pubmed
S - Hexylglutathione or the xenobiotic substrate analogue , 2,4 - dinitrophenol , protects against inactivation of the enzyme by 4 - FSB , whereas S - methylglutathione has little effect on the reaction . - pubmed
These experiments indicate that reaction occurs within the active site of the enzyme , probably in the binding site of the xenobiotic substrate , close to the glutathione binding site . - pubmed
Incorporation of [ 3,5 - 3H ]- 4 - FSB into the enzyme in the absence and presence of S - hexylglutathione suggests that modification of one residue is responsible for the partial loss of enzyme activity . - pubmed
DTT regenerates cysteine from the reaction product of cysteine and 4 - FSB , but does not reactivate the enzyme . - pubmed
These results show that modification of Tyr 8 by 4 - FSB causes the partial inactivation of the enzyme . - pubmed
The pH dependence of the enzyme - catalyzed reaction of glutathione with CDNB for the modified enzyme , as compared with the native enzyme , reveals an increase of about 0.9 in the apparent pKa , which has been interpreted as representing the ionization of enzyme - bound glutathione ; however , this pKa of about 7.4 for modified enzyme remains far below the pK of 9.1 for the -SH of free glutathione . - pubmed
Previously , it was considered that Tyr 8 was essential for GST catalysis . - pubmed
In contrast , we conclude that Tyr 8 facilitates the ionization of the thiol group of glutathione bound to glutathione S - transferase , but is not required for enzyme activity . - pubmed
The substitution at nucleotide 1168 ( G-->C ) generates one amino acid difference at residue 390 ( Asp-->His ) of the envelope protein ( E ) . - pubmed
Ancillary analyses of these trials implicate certain risk factors for the continued progression of CAD assessed by coronary angiography ; although standard lipids such as total cholesterol and LDL - cholesterol are important risk factors , recent evidence suggests that triglycride - rich lipoproteins are also important contributors to progression of CAD , both in treated and untreated trial subjects . - pubmed
Staphylococci express a 42 kDa cell - wall - associated protein which functions as a receptor for the mammalian iron - binding glycoprotein transferrin . - pubmed
However , small intestinal RNA content in the infected protein - free dietary group administered nucleoside - nucleotide mixture was higher ( P & lt ; 0.05 ) and tended to be higher relative to the infected nucleotide - free 20 % casein group administered nucleoside - nucleotide mixture compared with the rest of the groups . - pubmed
Formation of the ADP - insensitive phosphoenzyme intermediate in the sarcoplasmic reticulum Ca2 + - ATPase of which both Cys344 and Cys364 are modified by N - ethylmaleimide . - pubmed
Sarcoplasmic reticulum vesicles were pretreated with N - ethylmaleimide under the conditions in which both Cys344 and Cys364 ( SHD ) of the Ca2 + - ATPase are selectively modified . - pubmed
Effects of the modification on the transition of the phosphoenzyme intermediate ( EP ) from ADP - sensitive form to ADP - insensitive form and on the formation of ADP - insensitive EP from Pi were examined without added K+ . - pubmed
At pH 7.0 - 8.0 in totally aqueous media , the EP transition and the EP formation from Pi were almost completely inhibited by the SHD modification . - pubmed
The formation of ADP - insensitive EP from ATP and from Pi in the SHD - modified enzyme occurred to some extent at pH 6.0 - 6.5 and were greatly increased by addition of dimethyl sulfoxide at pH 6 . - pubmed
The inhibition by the SHD modification of the EP formation from Pi in the absence of dimethyl sulfoxide was attributed to a decrease in the equilibrium constant for the EP formation from the enzyme - Pi - Mg complex . - pubmed
When 40 % ( v / v ) dimethyl sulfoxide was present , almost all the phosphorylation sites in the SHD - modified enzyme were phosphorylated with ATP at pH 6.0 or with Pi at pH 6.0 - 7.0 , and all the EP formed was ADP - insensitive . - pubmed
These results lead to the possibility that the previously reported exclusion of water from the catalytic site upon the EP transition and upon the EP formation from the enzyme - Pi - Mg complex is inhibited by the SHD modification . - pubmed
The modification of SHD , which brackets the phosphorylation site ( Asp351 ) , may provide a useful tool for the analysis of conformational changes at the phosphorylation site occurring in the catalytic cycle . - pubmed
In contrast to the relatively stable PTH1R mRNA expression , the cAMP response to PTH varies markedly with no response at day 5 and a marked response ( 80 - fold versus control ) by day 10 . - pubmed
Since cAMP signaling was relatively unchanged during this interval ( day 10 - 30 ) and stimulation of adenylate cyclase only partially mimicked the PTH effect on increased mineralization , other signaling pathways are likely to be involved in order to determine the specific anabolic response to short - term PTH treatment during the differentiation process . - pubmed
Also , major basic protein , nitric oxide , and eotaxin were found in the tissue , and eosinophil cationic protein and eosinophil protein X levels were elevated in sera . - pubmed
Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors . - pubmed
It has recently been reported that N - ethylmaleimide - sensitive fusion ATPase ( NSF ) can fuse protein - free liposomes containing substantial amounts of 1,2 - dioleoylphosphatidylserine ( DOPS ) and 1 , 2 - dioleoyl - phosphatidyl - ethanolamine ( DOPE ) ( Otter - Nilsson et al . - pubmed
However , this activity of NSF is resistant to inactivation by N - ethylmaleimide and does not depend on the presence of alpha - SNAP ( soluble NSF - attachment protein ) . - pubmed
Moreover , under the same conditions , either alpha - SNAP , other proteins apparently unrelated to vesicular transport ( glyceraldehyde-3 - phosphate dehydrogenase or lactic dehydrogenase ) or even 3 mM magnesium ions can also cause lipid mixing . - pubmed
This transition can be mimicked in vitro , by F9 embryonal carcinoma ( EC ) cells treated with retinoic acid , to form ( epithelial ) primitive or visceral endoderm , and then with parathyroid hormone - related peptide ( PTHrP ) to induce the transition to ( mesenchymal ) parietal endoderm . - pubmed
Dye injection showed that this complex regulation of connexin43 is correlated with functional gap junctional communication . - pubmed
This mature phenotype is reached gradually during development through phases in which A - type potassium channels and T - type calcium channels are transiently expressed . - pubmed
A clear association between head pain and the release of calcitonin gene - related peptide ( CGRP ) was demonstrated . - pubmed
Structure - activity relationship of cinnamic acylsulfonamide analogues on the human EP3 prostanoid receptor . - pubmed
In Experiment 1 , the sipper tube conditioned stimulus ( CS ) contained saccharin / ethanol solution and was repeatedly paired with food as an unconditioned stimulus ( US ) . - pubmed
The CS - US paired group consumed more of the 0.1 % saccharin-6 % ethanol solution than did the CS - US random group , revealing that autoshaping conditioned responses ( CR ) induce ethanol drinking not attributable to pseudo - conditioning . - pubmed
Peptide antigen priming of naive , but not memory , CD8 T cells requires a third signal that can be provided by IL-12 . - pubmed
Challenge with peptide Ag in the absence of adjuvant results in tolerance of CD8 T cells specific for the Ag . - pubmed
Crystal structure of a clavaminate synthase - Fe ( II )- 2 - oxoglutarate - substrate - NO complex : evidence for metal centered rearrangements . - pubmed
Clavaminate synthase ( CAS ) , a 2 - oxoglutarate ( 2OG ) dependent dioxygenase , catalyses three steps in the biosynthesis of clavulanic acid . - pubmed
Decay products of the S(3 ) state of the oxygen - evolving complex of photosystem II at cryogenic temperatures . - pubmed
The water - oxidizing complex of photosystem II cycles through five oxidation states , denoted S(i ) ( ) ( i = 0 - 4 ) , during water oxidation to molecular oxygen , which appears at the ( transient ) S(4 ) state . - pubmed
Excitation of the S ( 3 ) state by near - IR light at cryogenic temperatures induces among other signals a derivative - shaped EPR signal at g= 5 [ Ioannidis , N. , and Petrouleas , V. ( 2000 ) Biochemistry 39 , 5246 - 5254 ] . - pubmed
A sorbitol dehydrogenase inhibitor of exceptional in vivo potency with a long duration of action : 1 -( R )-[ 4 -[ 4 -( 4,6 - dimethyl [ 1,3,5 ] triazin-2 - yl )- 2R,6S - dimethylpiperazin-1 - yl ] pyrimidin-2 - yl ] ethanol . - pubmed
These results suggest that eugenol may act by blocking Abeta - induced - Ca ( 2 + ) intake and provide a strong case for further pursuit of the therapeutic and prophylactic potentials of RAG and its active principles for the management of Alzheimer ' s disease . - pubmed
In this paper , it was studied if osmotic shrinkage of platelets affects the changes in intracellular calcium concentration ( [ Ca(2+)](i ) ) induced by thrombin or adenosine 5 ' diphosphate ( ADP ) . - pubmed
The osmolarity of the solution was elevated by the addition of 200 mM mannitol or sucrose and after the stimulation with 0.1 IU / ml of thrombin or 100 microM ADP , [ Ca ( 2 +)]( i ) increases were compared . - pubmed
RESULTS : It was found that hypertonic solutions decrease the [ Ca(2+)](i ) peak induced by the agonist : The increase of [ Ca ( 2 +)]( i ) in the presence of 200 mM mannitol produced by 100 microM ADP was 62+/-6 % and response to 0.1 IU / ml thrombin was 74+/-7 % of the increase in isotonic control solution . - pubmed
CONCLUSIONS : In spite of the stimulatory effect of hyperosmolarity on capacitative calcium entry observed in platelets in which the calcium stores were depleted with thapsigargin , the full response of intracellular calcium to the agonists tested ( ADP and thrombin ) was reduced by an increase in osmolarity . - pubmed
In a prospective , randomized , placebo - controlled 12 - wk study , we investigated the effect of oral hormone replacement therapy on asymmetric dimethylarginine ( ADMA ) , an endogenous inhibitor of nitric oxide synthase ( NOS ) , and an independent risk factor for coronary heart disease . - pubmed
In conclusion , unopposed oral estradiol and estradiol combined with dydrogesterone or trimegestone reduced plasma levels of the NOS inhibitor ADMA . - pubmed
Whether the reduction of the NOS substrate arginine in the E ( 2 )+ T group counteracts the potentially beneficial effect of ADMA reduction or reflects increased NO production remains to be investigated . - pubmed
[ Study of an X antigen complex from rhinopharyngeal exudate by means of an antigen - antibody reaction supported by erythrocytes ( passive hemagglutination reaction of the Cantacuzino - Angelescu type ) . - pubmed
Aldolase and glyceraldehyde-3 - phosphate dehydrogenase activity in primaquine - sensitive erythrocytes . - pubmed
mutans LraI operon , sloABCR , which encodes components of a putative metal uptake system composed of SloA , an ATP - binding protein , SloB , an integral membrane protein , and SloC , a solute - binding lipoprotein , as well as a metal - dependent regulator , SloR. - pubmed
By Western blotting , addition of Mn to the growth medium repressed SloC expression in a wild - type strain but not in a sloR mutant . - pubmed
Fe at 10 micro M supported growth of the wild - type strain but not of a sloA or sloC mutant . - pubmed
Mn at 0.1 micro M supported growth of the wild - type strain and sloR mutant but not of sloA or sloC mutants . - pubmed
The combined results suggest that the SloABC proteins transport both metals , although the SloR protein represses this system only in response to Mn . - pubmed
When tested for their sensitivity towards a series of antibiotics , null yts mutants were found to be sensitive to bacitracin . - pubmed
Real - time polymerase chain reaction ( PCR ) experiments demonstrated that the presence of bacitracin in the growth medium strongly stimulates the expression of the ytsCD genes encoding the ABC transporter and that this stimulation strictly depends on the YtsA response regulator . - pubmed
The ywoA gene encodes a protein known to confer some resistance to bacitracin on the bacterium . - pubmed
We propose that ( i ) the YtsCD ABC transporter exports the bacitracin ; ( ii ) YwoA , the protein that contains an acidPPc ( PAP2 or PgpB ) domain , is not part of an ABC transporter but competes with bacitracin for the dephosphorylation of the C55 - isoprenyl pyrophosphate ( IPP ) ; ( iii ) the two resistance mechanisms are independent and complementary . - pubmed
Long - term therapeutic regimes with ACTH and cortisone in chronic nonendocrine dyscrasias and the control of side reactions by intermittent and alternate low dosage with these pharmaceuticals . - pubmed
Correlation between oxygen - deficient center formation and volume compaction in synthetic SiO2 glass upon ArF or F2 excimer - laser irradiation . - pubmed
Correlations between the refractive - index change and the concentration of an oxygen - deficient center ( ODC ) induced by thermal treatments and laser irradiation are examined to clarify the origin of laser - induced volume compaction in synthetic SiO2 glasses . - pubmed
A linear correlation between them was clearly observed for thermally induced ODC , whereas no correlation was found for ArF or F2 laser irradiation . - pubmed
Transfection of phosphorothioate CAGA oligonucleotide , a CAGA antisense analog , inhibited Gly - BSA - induced PAI-1 mRNA expression . - pubmed
Cotransfection of phosphorothioate CAGA oligonucleotides with PAI-1 reporter vector also blocked Gly - BSA - induced PAI-1 promoter luciferase activity . - pubmed
We propose that Wun2 in pole cells competes with somatic Wun and Wun2 for a common lipid phosphate substrate , which is required by pole cells to produce their survival signal . - pubmed
On a quantitative basis the population of molecules used as immunogen always reacted most effectively with the homologous anti - idiotypic antiserum . - pubmed
Each cyanobacterial protein contains just one arginyl residue , similarly located between sites 1 and 2 , that is essential for the redox interaction with Photosystem I. - pubmed
Recruited macrophages specifically upregulated mRNA encoding the guidance molecule EphB3 and expressed EphB proteins capable of binding Ephrin B molecules in vivo and in vitro . - pubmed
In vitro , EphB3 supported adult RGC axon outgrowth , and axons turned toward a source of this guidance molecule . - pubmed
We show instead that STDP at layer 4 to layer 2/3 synapses in somatosensory ( S1 ) cortex involves separate calcium sources and coincidence detection mechanisms for LTP and LTD . - pubmed
LTD was independent of postsynaptic NMDA receptors and instead required group I metabotropic glutamate receptors and calcium from voltage - sensitive channels and IP3 receptor - gated stores . - pubmed
Downstream of postsynaptic calcium , LTD required retrograde endocannabinoid signaling , leading to presynaptic LTD expression , and also required activation of apparently presynaptic NMDA receptors . - pubmed
The enzyme showed essentially no activity toward hydroxypyruvate , pyruvate , or NADH . - pubmed
The K(m ) of the enzyme for glyoxylate was 0.11 millimolar . - pubmed
S - nitrosoglutathione ( SNOG ) accumulates hypoxia inducible factor - l alpha in main pulmonary artery endothelial cells but not in micro pulmonary vessel endothelial cells . - pubmed
A density functional theory ( DFT ) study was carried out to calculate ( 17 ) O , ( 14 ) N and ( 2 ) H electric field gradient ( EFG ) tensors in accurate neutron diffraction structures of alpha - glycine at 288 and 427 K. B3LYP is the used method and 6 - 311+G ( * ) and 6 - 311++G ( * * ) are the basis sets in the calculations of EFG tensors at the sites of ( 17 ) O , ( 14 ) N and ( 2 ) H nuclei in the monomer and the octameric cluster of alpha - glycine at two temperatures . - pubmed
The thermal decomposition of poly ( beta , l - malic acid ) , poly ( alpha - methyl beta , l - malate ) , and ionic complexes of the polyacid with alkyltrimethylammonium salts was studied by TGA , GPC , and FTIR and NMR spectroscopy . - pubmed
In this study , the effect of ( S )- 3,4 - dicarboxyphenylglycine ( DCPG ) , a selective mGlu8 receptor agonist , has been investigated in inflammatory and neuropathic pain models in order to elucidate the role of mGlu8 receptor in modulating pain perception . - pubmed
Intra - lateral PAG ( S )- 3,4 - DCPG reduced pain behaviour when administered 10 min before formalin or carrageenan ; both the effects were blocked by intra - lateral PAG MSOP . - pubmed
Taken together these results suggest that stimulation of mGlu8 receptors relieve formalin and carrageenan - induced hyperalgesia in inflammatory pain , whereas it would seem less effective in established inflammatory or neuropathic pain . - pubmed
This article reviews the evidence of efficacy ( or lack thereof ) for two types of agents touted as antiaging therapies : antioxidants ( vitamin E , vitamin C , and carotenoids ) and hormones ( growth hormone , testosterone , dehydroepiandrosterone , and vitamin D ) . - pubmed
The luciferase was biotinylated with conventional biotinylation reagents , and the biotinylated lysine sites were determined by liquid chromatography - tandem mass spectrometry . - pubmed
The biotinylated luciferase was stable when stored at 4 degrees C. The stability of synthetic S - Cypridina luciferin was significantly improved by adding antioxidants to Tris - HCl buffer . - pubmed
The biotinylated luciferase and S - Cypridina luciferin were used in a model study of the immunoassay for interferon - alpha . - pubmed
Structural elucidation of this impurity by spectral data ( 1H NMR , 13C NMR , MS and IR ) has been discussed . - pubmed
In conclusion , ligustrazine inhibits OVA induced airway inflammation by modulating key master switches GATA-3 and T - bet that result in reversing the Th2 cytokine patterns in asthma . - pubmed
A 13C - detected N - H dipolar coupling technique is introduced for uniaxially mobile membrane proteins for orientation determination using unoriented samples . - pubmed
LXR activation causes upregulation of genes involved in reverse cholesterol transport ( RCT ) , including ABCA1 and ABCG1 transporters , in macrophage and intestine . - pubmed
Meyer ) through repeated silica gel column chromatographies and its chemical structure was determined as ( 20S )- protopanaxadiol 3 - O -[ 6 ''- O - acetyl - beta - D - glucopyranosyl ( 1 - -& gt ; 2 )- beta - D - glucopyranoside on the basis of several spectral and physical evidences including HMBC and FAB - MS . - pubmed
The antiradical capacities of 13 carotenoids ( CAR ) and vitamin E are explored , by assessing CAR - H bond dissociation energy . - pubmed
Norfloxacin , trimethoprim / sulfamethoxazole , cytokines , nitric oxide , expression levels of nuclear factor ( NF )- kappaB and inhibitor of NF - kappaB ( IkB - alpha ) , neutrophil oxidative burst , and rate of apoptotic events were determined . - pubmed
Serum and ascitic levels of tumor necrosis factor - alpha , interferon - gamma , interleukin-12 , and nitric oxide were significantly lower in peak than in trough samples . - pubmed
A correlation was present between serum norfloxacins concentrations and tumor necrosis factor - alpha ( r = -0.68 ; P & lt ; .001 ) , interferon - gamma ( r = -0.66 ; P & lt ; .001 ) , interleukin-12 ( r = -0.66 ; P & lt ; .001 ) , and nitric oxide ( r = -0.68 ; P & lt ; .001 ) . - pubmed
Serum norfloxacin 's highest concentrations ( 1 + /- 0.5 microg / mL ) were achieved at 1 - 2 hours and concurred in time with the lower levels of cytokines and nitric oxide . - pubmed
Intracellular norfloxacin 's highest levels ( 2 + /- 1 microg / mL/10(7 ) cells ) were observed at 2 hours and concurred with a lower NF - kappaB expression , a reduced anion superoxide generation , and apoptotic rate in response to phorbol myristate acetate . - pubmed
Trimethoprim / sulfamethoxazole did not significantly modulate cytokine expression . - pubmed
AIM : To discuss current concepts in the use of fluoride and to determine how much fluoride is sufficient for caries prevention but also how much is too much . - pubmed
Local availability of glycine near N - methyl - D - aspartate receptors ( NMDARs ) is partly regulated by neuronal glycine transporter 1 ( GlyT1 ) , which can therefore modulate NMDAR function because binding to the glycine site of the NMDAR is necessary for channel activation . - pubmed
PKCbetaII , but not PKCbetaI , was localized to the mitochondria , and inhibition of PKCbeta significantly reduced UV - induced reactive oxygen species ( ROS ) in MCs with high PKCbeta expression . - pubmed
Geldanamycin selectively targets the nascent form of ERBB3 for degradation . - pubmed
Cellular studies indicate that , similar to EGFR , the geldanamycin ( GA ) sensitivity of ERBB3 and HSP90 binding resides in the nascent state and is dependent on the presence of the kinase domain of ERBB3 . - pubmed
Geldanamycin disrupts the interaction of ERBB3 and HSP90 and inhibits ERBB3 maturation at an early stage of synthesis , prior to export from the ER . - pubmed
Studies with a photo - convertible fusion protein of ERBB3 suggest geldanamycin sensitivity at a later stage in maturation , possibly through the putative role of HSP90 in structural proofreading . - pubmed
As TXNIP is strongly induced by glucose , this molecule could be of consequence to disordered angiogenesis manifest in diabetes mellitus . - pubmed
Magnetic resonance ( MR ) guided optical breast imaging is a promising modality to improve the specificity of breast imaging , because it provides high - resolution quantitative maps of total hemoglobin , oxygen saturation , water content , and optical scattering . - pubmed
We also identify Pellino 1 in a two - hybrid screen using IKKepsilon as bait , and show that IKKepsilon / TBK1 activate Pellino 1 in vitro by phosphorylating Ser76 , Thr288 and Ser293 . - pubmed
Proteins were covalently immobilized onto mesoporous TiO ( 2 ) film via 3 - aminopropyltriethoxysilane ( APTES ) , and protein loading onto TiO ( 2 ) films was about four times greater than on planar glass substrates , which consequently improved the protein activity . - pubmed
Specific binding assay between streptavidin / biotin and between PSA / anti - PSA demonstrated that the mesoporous TiO ( 2 )-based protein microarrays yielded higher fluorescence signals and were more sensitive with lower detection limits than microarrays based on planar glass slides . - pubmed
To overcome these limitations , high - dose intrathecal morphine was combined with a continuous intravenous ( IV ) postoperative naloxone infusion . - pubmed
The aim of the present chart analysis was to investigate the safety and efficacy of high - dose ITOs combined with IV naloxone compared with IV opioid analgesia alone . - pubmed
In the first 24 h , the ITO group used 6.8 + -10.2 morphine equivalents ( mg IV ) versus 76.1 + - 44.4 in the IVM group ( P<0.05 ) . - pubmed
CONCLUSIONS : High - dose ITOs combined with a postoperative IV naloxone infusion provided excellent analgesia for major pelvic surgery . - pubmed
The IV naloxone infusion combined with high - dose ITOs appeared to control opioid side effects without affecting analgesia . - pubmed
A HBSP has been designed as a novel bifunctional enzyme model with SOD and GPx activity by host / guest - directed self - assembly of MnTPyP - M - Ad and 6 - Te - diCD . - pubmed
In the bifunctional enzyme model , the Mn ( III ) porphyrins act as efficient SOD active sites and the tellurol moieties endow GPx activity . - pubmed
RESULTS : PI , BT and BV of biofilms treated by various concentrations of ZnCl ( 2 ) were reduced significantly when compared with the DDW group ( p<0.05 ) . - pubmed
PI , BT and BV of the 2.5mM ZnCl ( 2 ) group was significantly higher than groups of 5 , 10 , 20mM ZnCl ( 2 ) ( p<0.05 ) . - pubmed
Relationship between dose , drug levels , and D2 receptor occupancy for the atypical antipsychotics risperidone and paliperidone . - pubmed
Repeated 2 - week treatment with risperidone or paliperidone did not produce lasting changes in D2R binding potential in any region examined . - pubmed
We found dose - dependent D2R occupancy for both risperidone and paliperidone in both basal ganglia and cortical regions of interest . - pubmed
High resolution crystal structure of rat long chain hydroxy acid oxidase in complex with the inhibitor 4 - carboxy-5 -[( 4 - chlorophenyl ) sulfanyl ]- 1 , 2 , 3 - thiadiazole . - pubmed
Its isozyme glycolate oxidase ( GOX ) , has a role in the formation of oxalate , which can lead to pathological deposits of calcium oxalate , in particular in the disease primary hyperoxaluria . - pubmed
In comparison with a lower resolution structure of this enzyme , binding of the inhibitor induces a conformational change in part of the TIM barrel loop 4 , as well as protonation of the active site histidine . - pubmed
Plasma nicotine was measured by GC . - pubmed
LC - MS / MS was used to measure the plasma levels of cotinine and trans-3 '- hydroxycotinine , and urine levels of nicotine and its metabolites , total 4 -( methylnitrosamino )- 1 -( 3 - pyridyl )- 1 - butanol ( NNAL ) and PAH metabolites ( 2 - naphthol , 1 - , 2 - , and 3 - hydroxyfluorenes , 1 - , 2 - , 3 - , and 4 - hydroxyphenanthrenes , and 1 - hydroxypyrene ) . - pubmed
Furthermore , biochemical parameters of the redox status ( malondialdehyde , oxLDL and TEAC ) and C - reactive protein ( CRP ) in plasma and the concentrations of toxic gases and endotoxins in the air were determined . - pubmed
MDA , oxLDL and CRC levels were in both study groups similar . - pubmed
Synthesis and biological evaluation of ortho - aryl N - hydroxycinnamides as potent histone deacetylase ( HDAC ) 8 isoform - selective inhibitors . - pubmed
Enzyme inhibitory analysis demonstrated that some of the resulting compounds ( 22 b , 22 d , 22 f , and 22 g ) exhibited anti - HDAC8 activity superior to PCI34051 , a known HDAC8 - specific inhibitor , with IC(50 ) values in the range of 5 - 50 nM. - pubmed
A crossover from negative to positive junction magnetoresistance ( JMR ) is observed in p - La0.7Ca0.3MnO3 / SiO2 / n - Si heterostructure in current perpendicular to film plane ( CPP ) geometry . - pubmed
The specific aim was to demonstrate the involvement of the caspase family and to identify specific caspases which are activated by 6 - hydroxydopamine ( 6OHD ) treatment leading to death of dDCN . - pubmed
Western blot ( WB ) and double immunofluorescent analyses of caspases-2 , - 3 , and - 8 were carried out in untreated and 6OHD - treated dDCN . - pubmed
zVADfmk was used to determine if it could inhibit activation of caspases-2 , - 3 , and - 8 in dDCN following 6OHD - mediated stress . - pubmed
RESULTS : Our immunofluorescent and WB data showed that 6OHD triggered caspases-2 and - 8 activation which in turn activated caspase-3 leading to death of dDCN . - pubmed
Additionally , WB analysis revealed that caspases-2 , - 3 , and - 8 activation was reduced by zVADfmk in 6OHD - treated cells . - pubmed
DISCUSSION : The study showed that 6OHD - induced toxicity triggered caspase mediated death of dDCN . - pubmed
This finding is in support of previous studies using different PD model showing that 6OHD can induce caspases-2 and - 3 activation through apoptotic pathway and that both caspases can activate caspase-3 in PD . - pubmed
Anatoxin - a is one of the common and major cyanobacterial neurotoxins acting as a powerful agonist at nicotinic acetylcholine receptors ( nAChR ) . - pubmed
DESIGN , SETTING , PARTICIPANTS , & amp ; MEASUREMENTS : The prevalence of apparent treatment - resistant hypertension among Reasons for Geographic and Racial Differences in Stroke study participants treated for hypertension ( n=10,700 ) was determined by level of estimated GFR and albumin - to - creatinine ratio , and correlates of apparent treatment - resistant hypertension among those participants with CKD were evaluated . - pubmed
CKD was defined as an albumin - to - creatinine ratio & gt;= 30 mg / g or estimated GFR<60 ml / min per 1.73 m(2 ) . - pubmed
RESULTS : The prevalence of apparent treatment - resistant hypertension was 15.8 % , 24.9 % , and 33.4 % for those participants with estimated GFR & gt;= 60 , 45 - 59 , and & lt;45 ml / min per 1.73 m(2 ) , respectively , and 12.1 % , 20.8 % , 27.7 % , and 48.3 % for albumin - to - creatinine ratio<10 , 10 - 29 , 30 - 299 , and & gt;= 300 mg / g , respectively . - pubmed
The multivariable - adjusted prevalence ratios ( 95 % confidence intervals ) for apparent treatment - resistant hypertension were 1.25 ( 1.11 to 1.41 ) and 1.20 ( 1.04 to 1.37 ) for estimated GFR levels of 45 - 59 and & lt;45 ml / min per 1.73 m(2 ) , respectively , versus & gt;= 60 ml / min per 1.73 m(2 ) and 1.54 ( 1.39 to 1.71 ) , 1.76 ( 1.57 to 1.97 ) , and 2.44 ( 2.12 to 2.81 ) for albumin - to - creatinine ratio levels of 10 - 29 , 30 - 299 , and & gt;= 300 mg / g , respectively , versus albumin - to - creatinine ratio<10 mg / g. After multivariable adjustment , men , black race , larger waist circumference , diabetes , history of myocardial infarction or stroke , statin use , and lower estimated GFR and higher albumin - to - creatinine ratio levels were associated with apparent treatment - resistant hypertension among individuals with CKD . - pubmed
Logistic regression showed that compared with FTF , pregnant women interviewed via ACASI were significantly more likely to self - report themselves as single ( 14 % versus 6 % ) , having & gt;5 sexual partners ( 35 % versus 29 % ) , having oral sex ( 42 % versus 35 % ) , using intravenous drugs ( 5 % versus 0 ) , smoking cigarettes ( 23 % versus 16 % ) , drinking alcohol ( 13 % versus 8 % ) and using condoms during pregnancy ( 32 % versus 17 % ) . - pubmed
Relationship of apolipoproteins A-1 and B , and lipoprotein ( a ) to cardiovascular outcomes : the AIM - HIGH trial ( Atherothrombosis Intervention in Metabolic Syndrome with Low HDL / High Triglyceride and Impact on Global Health Outcomes ) . - pubmed
OBJECTIVES : This study sought to examine the relationship between baseline and on - study apolipoproteins ( apo ) A-1 and B and lipoprotein ( a ) [ Lp ( a ) ] levels and the development of subsequent cardiovascular ( CV ) events in the AIM - HIGH ( Atherothrombosis Intervention in Metabolic Syndrome with Low HDL / High Triglyceride and Impact on Global Health Outcomes ) trial . - pubmed
BACKGROUND : Niacin has been reported to lower apoB and Lp ( a ) and to raise apoA-1 . - pubmed
Baseline and on - study Lp ( a ) were predictive of CV events in both simvastatin plus placebo ( baseline HR : 1.24 [ p = 0.002 ] and on - study HR : 1.21 [ p = 0.017 ] ) and the simvastatin plus ERN group ( baseline HR : 1.25 [ p = 0.001 ] and on - study HR : 1.18 [ p = 0.028 ] ) . - pubmed
The photoluminescence ( PL ) quenching and blue shift in the peak of the emission spectra occur due to the energy transfer from Si NPs to Au NPs . - pubmed
Stress and withdrawal from d - amphetamine alter 5 - HT2A receptor mRNA expression in the prefrontal cortex . - pubmed
5 - HT2A receptor ( 5 - HT2AR ) mRNA expression in the prefrontal cortex ( PFC ) is diminished following withdrawal from d - amphetamine ( AMPH ) and may underlie the emotional and cognitive impairments observed in psychostimulant withdrawal , but whether stress affects 5 - HT2AR mRNA expression during psychostimulant withdrawal is unknown . - pubmed
The goal of this study was to examine the impact of forced swim test ( FST ) exposure during AMPH withdrawal on 5 - HT2AR mRNA expression in PFC . - pubmed
These data indicate that stress and short - term AMPH withdrawal affect prefrontal 5 - HT2AR mRNA expression to a similar degree , and stress experienced during long - term AMPH withdrawal can diminish the recovery of 5 - HT2AR mRNA expression . - pubmed
Together , these data suggest that exposure to stress during extended AMPH withdrawal could prolong withdrawal - induced , 5 - HT2AR mRNA expression which could be related to 5 - HT2AR mediated deficits . - pubmed
Here , we identified the methyl - CpG - binding domain protein 1 MBD1 as a physical and functional partner of the H19 long noncoding RNA ( lncRNA ) . - pubmed
The H19 lncRNA - MBD1 complex , through its interaction with histone lysine methyltransferases , therefore acts by bringing repressive histone marks on the differentially methylated regions of these three direct targets of the H19 gene . - pubmed
[ Anti - atherosclerosis role of N - oleoylethanolamine in CB2 ] . - pubmed
As the results shown , OEA ( 10 and 50 micromol x L(-1 ) ) could induce the CB2 protein and mRNA expression , but not 100 micromol x L(-1 ) . - pubmed
The anti - inflammation effect of OEA is induced by PPAR - alpha and CB2 , suggesting that CB2 signaling could be a target for anti - atherosclerosis , OEA have wide effect in anti - inflammation , it may have better therapeutic potential in anti - inflammation in HUVECs , thus achieving anti - atherosclerosis effect . - pubmed
The diagnostic phospholipid detected was phosphatidylcholine and MK-9 ( H4 ) was found to be the predominant menaquinone . - pubmed
Gene expression measurements revealed that paracetamol decreased the expression of antioxidant genes and increased the expression of interleukin-1beta ( IL-1beta ) , IL-8 , tumor necrosis factor - alpha ( TNF - alpha ) and acute phase proteins . - pubmed
Curcumin administration ameliorated paracetamol - induced alterations in genes expression of antioxidant and inflammatory cytokines . - pubmed
Gymnemic acid has a CAS number but its structure is not specified . - pubmed
We describe a one - pot strategy for the high yielding , operationally simple synthesis of fluorescent probes for Zn2 + that bear biological targeting groups and exemplify the utility of our method through the preparation of a small library of sensors . - pubmed
In both single and repetitive DE exposure studies , MaR1 significantly decreased bronchoalveolar lavage neutrophil infiltration , interleukin 6 , tumor necrosis factor alpha , and chemokine C - X - C motif ligand 1 levels without altering repetitive DE - induced bronchioalveolar inflammation or lymphoid aggregate formation . - pubmed
RESULTS : No significant differences for the modelled increase in CD4 + T - cell count ( placebo 15.3 CD4 + T cells / muL ( 95 % confidence interval ( CI ) [ 1.0 , 29.5 ] versus maraviroc arm 22.9 CD4 + T cells / muL ( 95 % CI [ 7.4 , 38.5 ] p = 0.51 ) or alterations in the expression of markers for T - cell activation , proliferation and microbial translocation were found between the arms . - pubmed
However , maraviroc intensification did increase the percentage of CCR5 expressing CD4 + and CD8 + T - cells , and the plasma levels of the CCR5 ligand MIP-1beta . - pubmed
In contrast , the percentage of ex - vivo apoptotic CD8 + and CD4 + T - cells decreased in the maraviroc arm . - pubmed
CONCLUSIONS : Maraviroc intensification of cART did not increase CD4 + T - cell restoration or decrease immune activation as compared to placebo . - pubmed
Amyloid deposition in AD dementia showed a preference for the DMN , but high effect sizes were also observed for other neocortical ICNs , most notably the frontoparietal - control network . - pubmed
The mechanisms involved included reduced oxidative stress , enhanced antioxidant capacity and restored vascular endothelial growth factor expression , and NO bioavailability . - pubmed
Sequential extractions were applied to determine the forms of P in different particle - size fractions and to assess the potential bioavailability of particulate phosphorus ( PP ) . - pubmed
The BAPP fluxes associated with the " truly suspended " fraction was approximately 200 times larger than the dissolved inorganic phosphorus ( DIP ) flux . - pubmed
Thus , the transfer of fine particles to the open sea is most probably accompanied by BAPP release to the DIP and can support greater primary and secondary production . - pubmed
Zinc sensing by metal - responsive transcription factor 1 ( MTF1 ) controls metallothionein and ZnT1 expression to buffer the sensitivity of the transcriptome response to zinc . - pubmed
Surprisingly , a greater number of genes were regulated by zinc following MFT1 knockdown , and most genes that responded to zinc under both control and MTF1 - depleted conditions had an augmented response in the latter condition . - pubmed
Exceptions were the zinc effluxer ZnT1 and a suite of metallothionein genes , suggesting that responses of other genes to zinc are usually buffered by increases in these proteins . - pubmed
We propose that MTF1 heads a hierarchy of zinc sensors , and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular zinc levels ( e.g. - pubmed
ZnT1 ) alters zinc buffering capacity and total cellular zinc content . - pubmed
We tested and validated this model by overexpressing metallothionein and observing the predicted curtailment in response of the zinc - repressed SLC30A5 ( ZnT5 ) promoter . - pubmed
venezuelensis were obtained in phosphate saline ( SS and SM ) and in Tris - HCl buffer ( TS and TM ) , and were analysed by Western blotting . - pubmed
Xanthine oxidase inhibitory activity of nicotino / isonicotinohydrazides : A systematic approach from in vitro , in silico to in vivo studies . - pubmed
Among the different factors involved in regulation of serum uric acid , xanthine oxidase ( XO ) is the best pharmacological target to control the levels of serum uric acid as it catalyzes the final steps in uric acid production . - pubmed
Benzylidene nicotino / isonicotinohydrazides ( 1 - 54 ) were synthesized by treating nicotinic / isonicotinic hydrazides with substituted aromatic aldehyde , and characterized by EI - MS and 1H NMR . - pubmed
Further in silico molecular docking was carried out to study the interactions of these inhibitors with catalytically important amino acid residues in XO . - pubmed
HMS and drug - loaded HMS were characterized by differential scanning calorimetry ( DSC ) , X - ray powder diffraction ( XRPD ) , Fourier transform infrared spectroscopy ( FTIR ) , scanning electron microscopy ( SEM ) , transmission electron microscopy ( TEM ) and contact angle study . - pubmed
The NBD - Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified LDL compared with other existing methods . - pubmed
Here , we focus on the invasive margin as defined by the region with 5 - aminolevulinic acid ( 5ALA ) ( GliolanTM ) fluorescence at surgery beyond the T1 enhancing region on magnetic resonance imaging ( MRI ) . - pubmed
We conducted hematoxylin and eosin ( H&amp;E ) , array real time polymerase chain reaction ( PCR ) , and immunohistochemistry staining on various intra - tumour regions of glioblastoma to determine molecular heterogeneity between regions . - pubmed
Mangiferin prevents the growth of gastric carcinoma by blocking the PI3K - Akt signalling pathway . - pubmed
Taken together , our findings indicate that mangiferin effectively inhibits cell growth and induces apoptosis of gastric cancer cells through inhibiting the PI3K / Akt pathways with relative safety , and may be used as a novel chemotherapeutic agent against gastric cancer . - pubmed
RESULT(S ): An implantation rate of 69.9 % , clinical pregnancy rate per transfer of 70.6 % , and live birth rate per transfer of 64.5 % were observed in 1,621 nondonor frozen cycles with the use of SNP - based PGT - A. - pubmed
No statistically significant differences in pregnancy outcomes were found for single - embryo transfers ( SET ) versus double - embryo transfers with SNP - based PGT - A. - pubmed
CONCLUSION(S ): Our findings that SNP - based PGT - A can mitigate the negative effects of maternal age on IVF outcomes in cycles with transfer , and that pregnancy outcomes from SET cycles are not significantly different from those of double - embryo transfer cycles , support the use of SET when transfers are combined with SNP - based PGT - A. - pubmed
BACKGROUND : Toxicological studies highlight the potential neurotoxicity of perfluoroalkyl substances ( PFAS ) during fetal development . - pubmed
RESULTS : Each ln - increase in perfluorononanoate ( PFNA ) at 8 years was associated with a 3.4 - point increase ( 95 % CI 0.4 , 6.3 ) in metacognition score , indicating poorer function . - pubmed
16 4903 ; Gu et al 2017 ACS Nano 11 4943 ) , here we study the strain performance for zigzag BlueP nanoribbons ( zBluePNRs ) with different edge chemical modifications . - pubmed
The limit of detection ( LOD ) and limit of quantification ( LOQ ) of the sensor were 1.76 pM and 5.87 pM for TNT and 1.74 pM and 5.80 pM for tetryl , respectively . - pubmed
The sensor could be selectively applied to various mixtures of TNT with common energetic materials such as RDX , HMX , and PETN . - pubmed
Here we report , for the first time , large volume analytical serial sectioning tomography of an organic composite coating using a xenon Plasma Focused Ion Beam ( PFIB ) combined with secondary electron imaging , energy dispersive X - ray ( EDX ) spectrum imaging ( SI ) and electron backscattered diffraction ( EBSD ) . - pubmed
During the seed - filling stage , the AMF - host PC with a reduced P application rate increased the uptake of most nutrients compared to the non - AMF - host PC , and improved the remobilization efficiency of all nutrients except Mn , Fe , and Se , compared to the optimal P application rate . - pubmed
Formaldehyde ( FA ) is involved in many biological processes and is closely connected with many diseases including Alzheimer ' s disease and cancer . - pubmed
In this manuscript , the first lysosome - targeted CDs for ratiometric fluorescence detection of FA were efficiently prepared from dexamethasone and 1,2,4,5 - tetraaminobenzene through the microwave - assisted hydrothermal method . - pubmed
With ISEL as our primary exposure variable , we performed multivariable regression models to evaluate the association between social support and prevalent CKD [ estimated glomerular filtration rate ( eGFR ) & lt ; 60 ml / min/1.73 m2 or urine albumin - creatinine ratio ( ACR ) & gt;=30 mg / g ] , eGFR decline , and rapid renal function decline ( RRFD ) ( & gt ; 30 % decrease in eGFR over approximately 10 years ) . - pubmed
While induction of testicular T / D remarkably increased the level of TNF - alpha in the ipsilateral ( torted ) and contralateral ( non - torted ) testes , intraperitoneal ( i.p ) administration of dapsone ( 12.5 mg / kg ) significantly lowered the TNF - alpha level ( p & lt ; 0.001 ) . - pubmed
Additionally , after induction of T / D , SOD activity was notably decreased , whereas administration of dapsone ( 12.5 mg / kg , i.p . ) - pubmed
Significant drug - AR - AE pair signals involving gabapentin included : drug abuser , multiple drug overdose , and substance - induced psychotic disorder . - pubmed
Significant drug AR - AE signals involving gabapentin and pregabalin , but not duloxetine , were : ataxia , dependence , drug abuse , increased drug tolerance , and overdose . - pubmed
Compared to duloxetine , gabapentin had significantly greater odds of a co - report for an AS - AE with drug withdrawal syndrome ( OR : 6.55 ) , auditory hallucinations ( OR : 4.57 ) , delusions ( OR : 2.36 ) , euphoric mood ( OR : 5.45 ) , ataxia ( OR : 2.85 ) , drug abuser ( OR : 3.01 ) , aggression ( OR : 1.98 ) , psychotic disorder ( OR : 1.96 ) , and feeling abnormal ( OR : 1.31 ) . - pubmed
Prevention of Bone Resorption by HA / beta - TCP + Collagen Composite after Tooth Extraction : A Case Series . - pubmed
The purpose of this study was to investigate the clinical efficacy of the application of a hydroxyapatite / beta - tricalcium plus collagen ( HA / beta - TCP + collagen ) dental bone graft in dental sockets immediately after tooth extraction , so as to prevent socket resorption . - pubmed
HA / beta - TCP + collagen was inserted into all of the dental sockets immediately after extraction , and was covered with a flap . - pubmed
In conclusion , the HA / beta - TCP + collagen graft demonstrated adequate safety and efficacy in dental socket preservation following tooth extraction . - pubmed
Then , the rewired TCA cycle was optimized by designing various strengths of pyruvate carboxylase and isocitrate dehydrogenase expression cassettes , resulting in a large increase in alpha - KG production ( 24.66 g / L ) . - pubmed
Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3'UTR of PTEN and Risk of Cervical Cancer Among the Han Chinese . - pubmed
Results : After adjusting for age , body - mass index , alcohol consumption , smoking , and familial history of cancer , the PTEN rs34140758 A allele carriers were 1.47 times more likely to suffer from cervical cancer than the C allele carriers ( odds ratio [ OR ] = 1.47 , 95 % confidence interval [ CI ] : 1.17 - 1.72 , p = 0.001 ) . - pubmed
It has been established that the action of MutT1 on 8 - oxo - dGTP , 8 - oxo - GTP and diadenosine polyphosphates is modulated by intermolecular interactions . - pubmed
In order to further explore this and to elucidate the structural basis of its differential action on 8 - oxo - NTPs and unsubstituted NTPs , the crystal structures of complexes of MsMutT1 with 8 - oxo - dGTP , GMPPNP and GMPPCP have been determined . - pubmed
Analysis of the structural data led to the elucidation of a relationship between the arrangements of molecules observed in the crystals , molecular plasticity and the action of the enzyme on nucleotides . - pubmed
Testosterone , dihydrotestosterone ( DHT ) , dehydroepiandrosterone ( DHEA ) and androstenedione were measured by IA and LC - MS / MS before and 7 days after rhCG injection . - pubmed
Using the PB method , all hormones showed a linear correlation , but proportional and systematic concordance errors were detected for androstenedione , systematic errors for testosterone and no errors for DHEA and DHT . - pubmed
By the BA method , there was a trend of IA to overestimate testosterone and androstenedione and underestimate DHEA and DHT when compared to LC - MS / MS . - pubmed
CONTEXT : 11beta - Hydroxysteroid dehydrogenase 1 ( 11betaHSD1 ) reduces inert cortisone into active cortisol but also catalyzes reverse dehydrogenase activity . - pubmed
MAIN OUTCOME MEASURE(S ): 11beta - Reductase and 11beta - dehydrogenase activities were measured during infusion of 9,11,12,12 - [ 2H ] 4 - cortisol and 1,2 - [ 2H ] 2 - cortisone , respectively , on two occasions , once during saline infusion and once during a hyperinsulinemic - euglycemic clamp . - pubmed
CONCLUSIONS : Regeneration of cortisol is up - regulated by insulin in adipose tissue but not skeletal muscle . - pubmed
In obesity , the equilibrium between 11beta - reductase and 11beta - dehydrogenase activities likely promotes cortisol accumulation in adipose , which may lead to adverse metabolic consequences . - pubmed
We show here that the sandwiched - fusion strategy , which provides robust protection against proteolysis , affords an economical method to obtain large quantities of pure five MDPs and the transcription factor Guy1 , in sharp contrast to otherwise unsuccessful recovery using the traditional amino - fusion method . - pubmed
The partial amino acid sequence of the light chain of one of these antibodies was identical with the dominant antibody light chain sequence , while the light chain of the other antibody , also partially established , showed significant variations in the framework - associated regions with identical CDRI and II . - pubmed
This report severe to document the histologic features of fatal exposure to sulfur dioxide and stresses the need to follow parameters of pulmonary function in the nonfatal cases . - pubmed
All demonstrable enzymes and toxins of encapsulated staphylococci ( KS ) were removed by extraction with guanidinium chloride . - pubmed
Angiotensin converting enzyme ( ACE ) is a halide activated peptidase produced mainly by endothelial cells and by activated monocytes . - pubmed
These results suggest that L - lactate is translocated through CAD - inhibitable new pathways induced in the host cell membrane by parasite activity , probably by diffusion of the acid form and through a modified native monocarboxylate : H + symporter . - pubmed
Continuous monitoring of extracellular pH changes occurring upon suspension of infected cells in isoosmotic Na - lactate solutions indicates that part of the lactate egress is mediated by anionic exchange through the constitutive , but modified , anion exchanger . - pubmed
Anesthesia was induced with SF , 6 micrograms / kg , while breathing 100 % oxygen . - pubmed
The structure and stereochemistry of 16 - hydroxytriptolide was established as L2 on the basis of spectral data ( IR , MS , UV , 1H - NMR , 13C - NMR , 2d - NMR , NOE ) and X - ray crystallographic analysis . - pubmed
Net mass transfer of cholesteryl esters from low density lipoproteins to high density lipoproteins in plasma from normolipidemic subjects . - pubmed
All plasma samples showed an increase in high density lipoprotein ( HDL ) cholesteryl esters during 1 - hour incubations . - pubmed
Transfer of triglycerides occurred from VLDL + LDL to HDL in plasma from all subjects . - pubmed
The cholesteryl ester transfer ( measured after 1 hour ) is not related to the activity of cholesteryl ester transfer protein . - pubmed
Ultracentrifugal separation of plasma lipoproteins revealed that both HDL and VLDL are acceptors of cholesteryl esters and that VLDL donates triglycerides to both LDL and HDL . - pubmed
Removal of VLDL from plasma by ultracentrifugation did not affect the cholesteryl ester transfer from LDL to HDL . - pubmed
We conclude that LDL may act as a donor of cholesteryl esters during incubation of normolipidemic plasma . - pubmed
Studies by flow cytometry have shown that these antigens are found on the surface of epidermal and endothelial cells , and are modulated by gamma interferon . - pubmed
The first step is characterized by ( i ) destabilization of the secondary acceptor semiquinone anion , Q - B , bound to the D1 protein . - pubmed
However , the slow phase ( microsecond ) of P+680 dark reduction increased , and the amplitude of signal II was reduced by 20 - 30 % , indicating that in a fraction of RCII , electron donation from Z to P + 680 was impaired without losing primary photochemistry . - pubmed
In conclusion , reducing catecholamine content or modifying beta - noradrenergic receptor activity was not able to alter the convulsive activity which occurs after acute PINX . - pubmed
[ Observation of oral glucose - insulin releasing test in liver cirrhosis ] . - pubmed
Blood glucose and immunoreactive insulin were determined in both groups at 60 min intervals for 180 min . - pubmed
To attain complete rest it is advisable to administer as premedication a psychopharmaceutical preparation and the neuroleptic preparation Haloperidol in adequate doses . - pubmed
Thermal stability of myosin subfragment-1 decreases upon tryptic digestion in the presence of nucleotides . - pubmed
Myosin subfragment-1 ( S-1 ) , digested with trypsin in the presence of ATP , rapidly loses its ATPase activity upon mild heat treatment even if ATP or ADP is present . - pubmed
Direct measurement of tubulin and bulk message distributions on polysomes of growing , starved and deciliated Tetrahymena using RNA gel blots of sucrose gradients containing acrylamide . - pubmed
The major translation - level process regulating tubulin synthesis in Tetrahymena appears to be a change in message loading mediated by a non - specific message recruitment or unmasking factor . - pubmed
Both HP6069 or HP6070 ( anti - IgG1 Fc ) worked well alone or together as capture antibodies in the IgG1 IEMA : WR = 20 - 1250 ng / ml , S = 15 ng / ml . - pubmed
HP6002 ( anti - gG2 Fc ) alone or in combination with HP6014 ( anti - IgG2 Fd ) produced an IgG2 IEMA with a WR of 5 - 200 ng / ml and S of 5 ng / ml . - pubmed
HP6047 ( anti - IgG3 hinge ) alone generated a sensitive IgG3 IEMA with a narrow working range : WR = 2 - 50 ng / ml , S = 1.6 ng / ml . - pubmed
Both HP6025 and HP6023 ( anti - IgG4 Fc ) worked equally well alone and together to produce a useful IgG4 IEMA : WR = 8 - 250 ng / ml , S = 7.8 ng / ml . - pubmed
HP6017 ( anti - IgG PAN Fc ) was combined in an equal molar ratio with HP6046 ( anti - IgG PAN Fd ) to produce a total IgG PAN IEMA with a WR of 5 - 530 ng / ml and a sensitivity of 5 ng / ml . - pubmed
Propionyl - CoA carboxylase [ PCC , propanoyl - CoA : carbon - dioxide ligase ( ADP - forming ) , EC 6.4.1.3 ] is a biotin - dependent enzyme involved in the degradation of branched - chain amino acids , fatty acids with odd - numbered chain lengths , and other metabolites . - pubmed
One class contained the anticipated Ala - Met - Lys - Met sequence , corresponding to the biotin binding site found in several biotin - dependent carboxylases , thus confirming the alpha - chain assignment of these clones . - pubmed
All three parasite lines required sialic acid for optimal invasion , but Thai-2 parasites cultured in Tn erythrocytes invaded neuraminidase - treated erythrocytes with 45 % efficiency whereas Camp parasites invaded neuraminidase - treated erythrocytes with less than 10 % efficiency . - pubmed
The experiments again call attention to the phenomenon that the steroid hormones , even though specific for their target cells , are able to induce " alien " imprinting when administered in the neonatal period . - pubmed
Cell - bound isozymes of penicillinase are distinguished from extracellular enzyme by their capacity to bind deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in its presence . - pubmed
In the case of plasma membrane penicillinase , addition of 25 per cent potassium phosphate at pH 9.0 leads to a 65 per cent conversion in 20 minutes at 30 degrees . - pubmed
One epitope was located between amino acid residues 145 - 168 of VP1 and the other between amino acids 169 - 179 . - pubmed
Incubations of horizontal sections through the hippocampus with [ 3H ] spiperone ( 1 nM ) resulted in dense labeling restricted to the pyramid cell layer in CA1 , the parasubiculum and layers I and II of the entorhinal area ( EA ) , while the other hippocampal subfields contained moderate to low binding . - pubmed
Complete traslocation of IF from the cytoplasm to the nuclear compartment was evident in all normal endometrial cells exposed to E2 , DES or OH - TX for two hours . - pubmed
While cells from the endometrial cancer 'S ' , like the normal cells , translocated IF to the nucleus , cells of another cancer ( ' KLE ' ) failed to translocate when exposed to E2 or OH - TX . - pubmed
The results revealed the suitability of anti - ER antibodies for investigating the intracellular dynamics of ER in target cells responding to estrogens or antiestrogens . - pubmed
Reductive methylation of the lysyl residues in the fd gene 5 DNA - binding protein : CD and 13C NMR results on the modified protein . - pubmed
The epsilon - amino groups of the six lysyl residues of the fd gene 5 DNA - binding protein have been modified by reductive methylation to form N epsilon , N epsilon - dimethyl lysyl derivatives containing 13C - labeled methyl groups . - pubmed
13C NMR spectra at 50.1 MHz of [ 13C ] methylated gene 5 protein show five distinct resonances between 43.30 and 42.76 ppm originating from the six N epsilon , N epsilon - dimethyl lysyl residues . - pubmed
The optimal pH and phosphate concentration for the separation of hemoglobin from the membranes were found to be 7.5 and 5 mM , respectively . - pubmed
Sodium dodecyl sulphate ( SDS ) electrophoretic analysis of the membrane proteins prepared by centrifugation and by this new method shows similar protein compositions . - pubmed
The isolated cDNA was identical in both nucleotide and amino acid sequences with the recently cloned liver dihydrodiol dehydrogenase ( Stolz , A. , Hammond , L. , Lou , H. , Takikawa , H. , Ronk , M. , and Shively , J.E. - pubmed
The glycosylphosphatidylinositol ( GPI)-anchored membrane protein urokinase plasminogen activator - receptor ( uPA - R ; CD87 ) is one of the key molecules involved in migration of leukocytes and tumor cells . - pubmed
Activation of monocytes even with enzymatically inactivated uPA resulted in induction of tyrosine phosphorylation , suggesting modulation of uPA - R - associated PTKs upon ligand binding . - pubmed
[ The DEC-205 receptor expressed by dendritic cells and thymic epithelial cells is implicated in antigen processing ] . - pubmed
Acetylcholinesterase inhibition by zifrosilone : pharmacokinetics and pharmacodynamics . - pubmed
Serial blood samples were obtained for zifrosilone plasma concentrations and red blood cell acetylcholinesterase and butyrylcholinesterase activities . - pubmed
Butyrylcholinesterase activity was relatively unaffected by zifrosilone ( & lt ; 20 % inhibition at 300 mg ) . - pubmed
For doses & gt ; or = 200 mg , an Emax pharmacodynamic model was used to describe the relationship between zifrosilone plasma concentration and red blood cell acetylcholinesterase inhibition ( Emax = 83.8 % ; EC50 = 0.65 ng / ml ) . - pubmed
CONCLUSIONS : Zifrosilone showed dose - dependent pharmacokinetics after oral administration and was effective in causing selective inhibition of red blood cell acetylcholinesterase . - pubmed
Metabolism of cholecystokinin-33 in vivo : effect of L-364,718 , a CCK receptor antagonist . - pubmed
Pretreatment of animals with erythro-9 -) 2 - hydroxy-3 - nonyl ) adenine ( EHNA ) , an inhibitor of adenosine deaminase , also potentiated the increased concentrations of adenosine in venous blood by 6 - fold and raised arterial adenosine concentrations by 3 - fold . - pubmed
These data clearly suggest that hemorrhage induced extensive adenosine release and the released endogenous adenosine was eliminated quickly from plasma by the uptake mechanism and adenosine deaminase . - pubmed
Immunogold labeling of thylakoid vesicles , with antibodies to synthetic peptides , shows that residues 413 - 421 of PSI - A are exposed on the stromal surface of the membrane , and that the accessibility of this region is enhanced by NaSCN treatment , which removes extrinsic polypeptides . - pubmed
Assuming that the transmembrane regions are correctly predicted by the 11 - helix model , the N - terminal domain , as well as the conserved region proposed to bind the iron - sulfur center FX , would be expected to be on the stromal surface . - pubmed
This level of interaction is in agreement with the functional behaviour of natural haemoglobin mutants with mutations at the 2,3 - diphosphoglycerate binding site . - pubmed
It was observed that during growth of this organism , sucrose in the medium is hydrolysed to glucose and fructose , suggesting the formation of an extracellular invertase . - pubmed
The invertase was found to be the only predominant extracellular protein in the culture broth and could be purified in a single step by precipitation at 90 % ammonium sulphate saturation . - pubmed
It not only showed invertase activity , but also a fructosyltransferase activity as it could convert sucrose to beta-1,2 - difructose , as well as to glucose and fructose . - pubmed
The power spectrum analysis of R - R interval variability ( RRV ) has been estimated by means of an autoregressive method in six men in supine ( S ) and sitting ( C ) postures at rest and during steady - state cycle exercise at about 14 % , 28 % , 45 % , 67 % of the maximal oxygen consumption ( % VO2max ) . - pubmed
Substitution of alanine543 with a threonine residue at the carboxy terminal end of the beta - chain is associated with thermolabile hexosaminidase B in a Jewish family of Oriental ancestry . - pubmed
The beta - chain thermolability was found to be associated with the presence of a G --> ; A transition at nucleotide 1627 of the HEX B gene causing the substitution of Ala543 with a threonine . - pubmed
Cb1 , the protein product of c - cbl proto - oncogene , has been reported to be phosphorylated on tyrosine residues upon T cell receptor ( TCR ) engagement . - pubmed
However , in lymphocytes activated through the TCR , Cbl loses its ability to bind to Grb2 precipitated either with anti - Grb2 antibody or with an immobilized tyrosine phosphopeptide , Y1068 - P , derived from the epidermal growth factor receptor . - pubmed
Additionally , high levels of hydrophobic amino acids were present , accounting for the hydrophobic nature of Bac1829 . - pubmed
The same effect was seen in the tps1 ( trehalose-6 - phosphate synthase ) hsp104 double mutant , suggesting that the extreme heat shock sensitivity was due mainly to a lack of trehalose rather than to the presence of trehalose-6 - phosphate . - pubmed
However , accumulation of trehalose-6 - phosphate did cause some phenotypes in the tps2 mutant , such as temperature sensitivity for growth . - pubmed
Finally , we isolated a high copy number suppressor of the temperature sensitivity of tps2 , which we call PMU1 , which reduced the levels of trehalose-6 - phosphate in tps2 mutants . - pubmed
CR3 may function to activate cells by presenting LPS to a downstream signal transducer . - pubmed
We conclude that in a selected group of patients without CC resistance and normal ovarian response following CC stimulation [ maximum of three follicles with a diameter of & gt;16 mm at the time of administration of human chorionic gonadotrophin ( HCG ) ] , IUI combined with CC - HCG can be offered as a very safe and non - expensive first - line treatment , at least with an IMC of & gt;1 x 10(6 ) spermatozoa . - pubmed
Only beta - sheet , positive ion salt bridges between phosphates , and hydrophobic association can create tangles of just AD P - tau . - pubmed
We have correlated transmission electron microscope ( TEM ) images of tau aggregation with different percentages of beta - sheet in aqueous suspensions of tau while using buffers that block dispositive or tripositive ionic bridges between intermolecular phosphates . - pubmed
Each preparation except one demonstrated an inverse temperature transition , Ti , in the CD at 197 nm . - pubmed
Occupation of five to nine phosphate sites on AD P - tau appears sufficient to reduce or neutralize tau 's basic character . - pubmed
The Ti for AD P - tau was 24.5 degrees C or 28 degrees C , whereas for B tau with three phosphates it was 32 degrees C , for unphosphorylated tau R-39 it was 38 degrees C , and for dephosphorylated HF AD P - tau it was 37.5 degrees C. The hydrophobic protein elastin and its analogs coalesce and precipitate at their Ti of 24 - 29 degrees C , well below body temperature . - pubmed
Combination therapy with thymosin alpha1 and interferon for the treatment of chronic hepatitis C infection : a randomized , placebo - controlled double - blind trial . - pubmed
We evaluated the addition of thymosin alpha1 ( TA1 ) , an immunomodulatory peptide , to the standard treatment regimen for hepatitis C to determine if combination therapy shows biological activity using outcome measures including normalization of alanine aminotransferase levels , histological activity , and viral load during treatment . - pubmed
The polyrotaxanes regulated thrombin - induced calcium increase in platelets although constituent molecules of the polyrotaxanes showed fewer effects on the intracellular metabolism . - pubmed
The mutation , a single A-->G base substitution at nucleotide 5519 of the SCN5A cDNA , is expected to cause a nonconservative change from an aspartate to a glycine at position 1790 ( D1790 G ) of the SCN5A gene product . - pubmed
Induction of the cell cycle regulatory gene p21 ( Waf1 , Cip1 ) following methylmercury exposure in vitro and in vivo . - pubmed
Both embryonic LB and CNS cells responded to MeHg exposure with a concentration - dependent increase in p21 mRNA . - pubmed
The ethanol extraction procedure concentrated CP , gross energy , and TMEn . - pubmed
7alpha,15alpha - Dihydroxyderivative was the only product isolated from the 5 - androsten-3beta - ol post - transformation mixture . - pubmed
The ( -)-nicotine ( 3x10(-5 ) M)-induced noradrenaline release was abolished by tetrodotoxin and hexamethonium and partially blocked by dihydro - beta - erythroidine ( up to 10(-5 ) M ) ( a relatively selective antagonist of alpha4beta2 nicotinic receptors ) and abolished by mecamylamine ( 10(-5 ) M ) ( a relatively selective antagonist of alpha3beta4 nicotinic receptors ) , but not influenced by alpha - bungarotoxin ( 3x10(-7 ) M ) or alpha - conotoxin ImI ( 10(-6 ) M ) ( antagonists of alpha7 nicotinic receptors ) . - pubmed
( + /-)- Epibatidine ( 3x10(-7 ) M ) ( a very potent , but non - selective agonist ) and ( -)- cytisine ( 3x10(-4 ) M ) ( an agonist of beta4 nicotinic receptors ) effectively evoked the release of noradrenaline , while ( E )- N - methyl-4 -( 3 - pyridinyl )- 3 - butene-1 - amine ( RJR-2403 ) ( up to 10(-4 ) M ) ( an agonist of alpha4beta2 nicotinic receptors ) had no effect . - pubmed
These subjects with low hematocrit had also higher values of the following parameters : aerobic working capacity ( p & lt ; 0.01 ) ; isometric adductor strength ( p = 0.02 ) ; crossover point of carbohydrate oxidation ( 70 % carbohydrates/30 % lipids ) ( p & lt ; 0.05 ) ; insulin like growth factor binding protein 1 ( p & lt ; 0.0001 ) . - pubmed
parasiticus resulted in the loss of fluP transcript , a 3- to 4 - fold reduction in hyphal growth rate , the appearance of a fluffy , cotton - like hyphal morphology , reduction or elimination of asexual spores and spore - bearing structures , and a twofold reduction in aflatoxin accumulation . - pubmed
The role of antiganglioside IgM is to eliminate the immunosuppressive gangliosides shed from tissues during ageing , degeneration of neural and extraneural tissues , and tumor growth and necrosis . - pubmed
Cyr61 is overexpressed , estrogen - inducible , and associated with more advanced disease . - pubmed
Differential screening of the library isolated the growth factor - inducible immediate - early gene Cyr61 , a secreted , cysteine - rich , heparin binding protein that promotes endothelial cell adhesion , migration , and neovascularization . - pubmed
Induction of Cyr61 mRNA was blocked by tamoxifen and ICI182,780 , inhibitors of the estrogen receptor . - pubmed
In summary , these results suggest that Cyr61 may play a role in the progression of breast cancer and may be involved in estrogen - mediated tumor development . - pubmed
Kinetic analysis of [ ( 11 ) C ] MP4A using a high - radioactivity brain region that represents an integrated input function for measurement of cerebral acetylcholinesterase activity without arterial blood sampling . - pubmed
[ ( 11 ) C ] MP4A is specifically hydrolyzed by AChE in the brain to a hydrophilic metabolite , which is irreversibly trapped locally in the brain . - pubmed
These regions , because of their high AChE activity , act as a biologic integrator of plasma input function during PET scanning , when regional metabolic rates of [ ( 11 ) C ] MP4A through AChE ( k(3 ) ; an AChE index ) are calculated by using Blomqvist 's linear least squares analysis . - pubmed
The sensitivity of RLS analysis for detecting cortical regions with abnormally low k 3 in the 26 patients with AD ( 138 of 312 regions , 44 % ) was somewhat less than NLS analysis ( 52 % ) , but was greater than shape analysis ( 33 % ) , another method of [ ( 11 ) C ] MP4A kinetic analysis without blood sampling . - pubmed
The authors conclude that RLS analysis is practical and useful for routine analysis of clinical [ ( 11 ) C ] MP4A studies . - pubmed
Pycnogenol protects neurons from amyloid - beta peptide - induced apoptosis . - pubmed
Reactive oxygen species ( ROS ) has been proposed to be involved in the apoptotic mechanism of Abeta - mediated neurotoxicity . - pubmed
We further found that PYC not only suppressed the generation of ROS but also attenuated caspase-3 activation , DNA fragmentation , PARP cleavage , and eventually protected against Abeta - induced apoptosis . - pubmed
Vitamin E also suppressed cell death and caspase-3 activation induced by Abeta(25 - 35 ) . - pubmed
A novel subclass of thalidomide analogue with anti - solid tumor activity in which caspase - dependent apoptosis is associated with altered expression of bcl-2 family proteins . - pubmed
The antitumor activity was independent of known PDE4 inhibitory activity and did not involve cAMP elevation . - pubmed
Thyrotropin ( TSH , 10 mU / ml ) and free calcium ( 0.17~1.95 mM ) , which are associated with the biological behavior of thyrocytes and C cells , respectively , did not affect the events described above . - pubmed
The data indicate that thyrocytes , but not C cells , actively undergo growth and three types of mother follicle - derived folliculogenesis at the tissue periphery in a TSH- or free calcium - independent manner . - pubmed
Detection of nitric oxide ( NO ) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals , because some are known to induce NO synthase ( iNOS ) activity thereby increasing NO production . - pubmed
The result of preoperative stress - rest Tc-99m - MIBI SPECT myocardial perfusion study is an exact predictor of the state of perfusion measured early ( & lt ; 5 months ) after CABG ; the postoperative regional perfusion improvement is most dependent upon reversibility and also upon severity of stress defect . - pubmed
Preoperative viability assessment on the basis of Tc-99m - MIBI study performed solely in rest is unjustified : at the similar perfusion defect at rest , the presence of even minimal inducible ischaemia is associated with increased probability of perfusion improvement after CABG . - pubmed
Induction by hypoxia combined with low glucose or low bicarbonate and high posttranslational stability upon reoxygenation contribute to carbonic anhydrase IX expression in cancer cells . - pubmed
We found that both hypoxia - induced transcription and CA IX protein level are further increased by reduced glucose or bicarbonate concentrations . - pubmed
However , by birth , the SN DA neurons were labeled exclusively by DiA placed in the dorsolateral striatum , and the VTA DA neurons were labeled only by DiI injected into the ventromedial striatum . - pubmed
Compared with CCK2R , CCK2i4svR contains an additional 69 amino acids within its third intracellular loop ( 3il ) domain . - pubmed
The peptides P1 , P2 , and P3 revealed a significant stimulatory effect on [ 35S]GTPgammaS binding in control temporal cortex . - pubmed
The most potent stimulator , P3 , at 10 microM concentration enhanced [ 35S ] GTPgammaS binding by 500 % . - pubmed
Glutathione , desferrioxamine ( DFO ) , and 17beta - estradiol markedly decreased the strong stimulatory effect by P3 on [ 35S ] GTPgammaS binding to control temporal cortex , with the protective effect by DFO being most potent . - pubmed
An IV bolus of lidocaine 2 mg / kg was then administrated and venous plasma lidocaine concentrations were measured to determine pharmacokinetic values . - pubmed
, OxyR ) and a low - molecular - weight heat shock protein ( HSP33 ) , whose activity increases upon oxidation of critical sulfhydryl residues . - pubmed
Depletion of cellular reduced glutathione may act as a signal for HO-1 transcriptional activation . - pubmed
HO-1 protein expression can occur in the lung in response to oxidative stress associated with infection , altered oxygen tension , and inflammatory diseases . - pubmed
Thiol - reactive clenbuterol analogues conjugated to bovine serum albumin . - pubmed
Several novel thiol - reactive clenbuterol analogues were coupled in high yield with bovine serum albumin ( BSA ) . - pubmed
After labelling of unreacted cysteines with maleimide spin label ( MiSL ) , the yield of the coupling reaction was determined by electron paramagnetic resonance ( EPR ) spectroscopy and spectral analysis . - pubmed
Molecular dynamics was used to model the structure of clenbuterol analogues and spin label conjugated to BSA and recognition of conjugates by anti - clenbuterol antibodies was demonstrated . - pubmed
The recognition of BSA - A , BSA - C and BSA - S conjugates with monoclonal and polyclonal anti - clenbuterol ( mCLB - Ab and rCLB - Ab ) antibodies was an indication , that chlorine substituents on the aromatic ring of clenbuterol derivatives are not necessary for the binding of antibodies to the conjugates . - pubmed
We show that an identical toeprint was found for the chloramphenicol acetyltransferase transcript using this nonradioactive method , which is in agreement with the previously published 32P - labeled method . - pubmed
Optimization of the safety and efficacy of interferon beta 1b and azathioprine combination therapy in multiple sclerosis . - pubmed
Linopirdine was developed as a cognitive enhancing molecule and demonstrated to specifically block the potassium current generated by the brain specific KCNQ2 - KCNQ3 proteins ( M - channel ) . - pubmed
The testing of CYP450 ligands on the M - channel activity , using a Rb(+ ) efflux assay on cells expressing the KCNQ2 - KCNQ3 proteins , demonstrated that [ ( 3 ) H ] linopirdine binding results can not be correlated to M - channel inhibition . - pubmed
Thermal dissipation of excess absorbed light energy , measured as non - photochemical quenching ( NPQ ) of chlorophyll fluorescence , is induced rapidly in response to excess light conditions , and it is known that xanthophylls such as zeaxanthin and lutein , the transthylakoid pH gradient , and the PsbS protein are involved in this mechanism . - pubmed
reinhardtii genes encoding PsbS and lycopene varepsilon - cyclase , and we show that the lor1 mutation , which affects lutein synthesis , is located within the lycopene varepsilon - cyclase gene . - pubmed
Pulmonary function tests were as follows ( mean+/-sd ): total lung capacity 88%+/-17 , forced vital capacity ( FVC ) 88%+/-18 , forced expiratory volume in one second ( FEV1 ) 80%+/-21 ( % predicted ) , FEV1 / FVC 69%+/-13 , carbon monoxide diffusion capacity of the lung 37%+/-16 ( % predicted ) , carbon monoxide transfer coefficient 46%+/-19 . - pubmed
The aim of this study was to enhance the efficiency of transfection and expression in BCECs of DNA / polymer nanoparticles with the modification of membrane - penetrating peptide , Antennapedia peptide ( Antp ) polyethylenimine ( PEI ) and polyamidoamine ( PAMAM ) were chosen to prepare Antp - modified DNA - loaded nanoparticles with a complex coacervation technique . - pubmed
After a 20 - min transfection , the efficiency , in terms of transfection and expression , of DNA / PEI NP or DNA / PAMAM NP was enhanced significantly with the modification of Antp . - pubmed
After a 3 - h transfection of DNA / Antp / PEI NP , there was no difference in cellular uptake but an enhancement in gene expression , compared to DNA / PEI NP alone . - pubmed
However , both the transfection and expression efficiency of DNA / PAMAM NP were enhanced using Antp . - pubmed
Cyanidase is a new enzyme preparation capable of degrading cyanide in industrial wastewaters to ammonia and formate in an apparently one - step reaction , down to very low concentrations . - pubmed
This enzyme has both a high selectivity and affinity toward cyanide . - pubmed
The kinetics of the enzymatic degradation of cyanide were studied in a batch reactor using the powdered immobilized enzyme preparation and modeled using a simple Michaelis - Menten equation . - pubmed
Heme - binding to the nuclear receptor retinoid X receptor alpha ( RXRalpha ) leads to the inhibition of the transcriptional activity . - pubmed
Heme acts as a ligand for transcription factors and regulates the expression of several genes . - pubmed
We here show that heme binds to RXRalpha and impairs its DNA - binding activity . - pubmed
The DNA - binding activity using the DR-1 consensus sequence of RXRalpha in electrophoretic mobility shift assays was inhibited by heme . - pubmed
However , the inductions of adipogenic factor mRNAs including PPAR - gamma , fatty acid binding protein-4 and glucose transporter-4 were markedly repressed by heme during adipocyte differentiation . - pubmed
Fast pressure jumps can perturb calcium and magnesium binding to troponin C F29W. - pubmed
We have used rapid pressure jump and stopped - flow fluorometry to investigate calcium and magnesium binding to F29W chicken skeletal troponin C. - pubmed
We sequenced the encoded complex I units : ND2 , ND3 , ND4 , ND5 and ND6 genes and the mitochondrial ATPase 6 , tRNA ( Val ) , tRNA(Leu(UUR ) ) , tRNA(Trp ) and tRNA(Lys ) genes in 10 unrelated patients with Leigh syndrome . - pubmed
Although the mechanism of idiosyncratic hepatotoxicity remains unknown , it is proposed that metabolic activation of nevirapine and subsequent covalently binding of reactive metabolites to cellular proteins play a causative role . - pubmed
These findings are consistent with a bioactivation sequence involving initial P450 - catalyzed dehydrogenation of the aromatic nucleus with a 4 - methyl group in nevirapine to an electrophilic quinone methide intermediate , which is subsequently attacked by glutathione yielding the sulfydryl conjugate . - pubmed
Formation of the nevirapine GSH conjugate was primarily catalyzed by heterologously expressed recombinant CYP3A4 and , to a lesser extent , CYP2D6 , CYP2C19 , and CYP2A6 . - pubmed
In addition , the quinone methide reactive metabolite was a mechanism - based inactivator of CYP3A4 , with inactivation parameters K(I ) = 31 microM and k(inact ) = 0.029 min(-1 ) , respectively . - pubmed
Phosphorylation of myristoylated alanine - rich C kinase substrate is involved in the cAMP - dependent amylase release in parotid acinar cells . - pubmed
The involvement of MARCKS phosphorylation in secretory function has been reported in Ca ( 2 +)-mediated exocytosis . - pubmed
The beta - adrenergic agonist isoproterenol ( IPR ) induced MARCKS phosphorylation in a time - dependent manner . - pubmed
Translocation of a part of phosphorylated MARCKS from the membrane to the cytosol and enhancement of MARCKS phosphorylation at the apical membrane site induced by IPR were observed by immunohistochemistry . - pubmed
H89 , a cAMP - dependent protein kinase ( PKA ) inhibitor , inhibited the IPR - induced MARCKS phosphorylation . - pubmed
The PKCdelta inhibitor rottlerin inhibited the IPR - induced MARCKS phosphorylation and amylase release . - pubmed
IPR activated PKCdelta , and the effects of IPR were inhibited by the PKA inhibitors . - pubmed
A MARCKS - related peptide partially inhibited the IPR - induced amylase release . - pubmed
These findings suggest that MARCKS phosphorylation via the activation of PKCdelta , which is downstream of PKA activation , is involved in the cAMP - dependent amylase release in parotid acinar cells . - pubmed
R (+) XK469 inhibits hydroxylation of S - warfarin by CYP2C9 . - pubmed
INTRODUCTION : XK469 is a novel topoisomerase II inhibitor structurally akin to several propionic acid derivatives , such as ibuprofen and diclofenac , which are metabolised by CYP2C9 . - pubmed
The aim of the study is to investigate whether R (+) XK469 interacts with S - warfarin by inhibition of CYP2C9 . - pubmed
This suggests that coadministration of R (+) XK469 and warfarin results in a clinically significant pharmacokinetic interaction due to CYP2C9 inhibition by R (+) XK469 . - pubmed
Although there was a significant correlation between BMD and SUV in the lumbar spine at baseline ( P & lt ; 0.05 ) , there was no correlation between the 2 variables at 12 mo of treatment with alendronate . - pubmed
Aerobic ammonia- and nitrite - oxidising bacteria were verified in water column samples through Most Probable Number ( MPN ) analysis , but they were not detected in sediment samples by FISH . - pubmed
The SRL interacts with translation factors that hydrolyze GTP , and it is important for their binding to the ribosome , but its precise role is not yet understood . - pubmed
alpha - Sarcin - treated ribosomes showed no defects in mRNA and tRNA binding , peptide - bond formation and sparsomycin - dependent translocation . - pubmed
Importantly , cleavage of SRL inhibited EF - G binding , and consequently GTP hydrolysis and mRNA - tRNA translocation . - pubmed
We report on a screening of 23 surgically removed , cortisol - secreting ATs for the expression of receptors for luteinizing hormone ( LH ) , gastric - inhibitory polypeptide ( GIP ) , and vasopressin ( V ( 1a ) , V ( 1b ) , and V ( 2 ) ) . - pubmed
In addition , polymorphisms have been described in modulatory systems involving the NMDAR , including the enzymes serine racemase and d - amino acid oxidase / G72 that regulate brain d - serine synthesis . - pubmed
BACKGROUND / AIMS : We have previously demonstrated that a transcription factor , upstream stimulatory factor 2 ( USF2 ) , regulates glucose - induced thrombospondin 1 expression and transforming growth factor - beta activity in mesangial cells , and plays an important role in diabetic glomerulopathy . - pubmed
In this study , we determined whether USF2 expression in renal proximal tubular cells is regulated by glucose and contributes to diabetic tubulointerstitial fibrosis . - pubmed
In addition , high glucose stimulated angiotensinogen and renin expression , increased renin activity , and resulted in increased angiotensin II formation . - pubmed
AluYb8 insertion in the MUTYH gene is related to increased 8 - OHdG in genomic DNA and could be a risk factor for type 2 diabetes in a Chinese population . - pubmed
Therefore , the AluYb8MUTYH polymorphism could be a novel genetic risk factor for T2DM , and accumulated 8 - OHdG could contribute to this disease . - pubmed
Inverse probability weighting to control for censoring in a post hoc analysis of quality - adjusted survival data from a clinical trial of temsirolimus for renal cell carcinoma . - pubmed
Differences in mean QAS between temsirolimus and IFN - alfa were evaluated with t - statistics at a two - sided alpha = 0.05 . - pubmed
Overall weighted mean ( standard deviation ) QAS during progression - free survival was 111.9 ( 5.3 ) days with temsirolimus ( n = 204 ) and 75.7 ( 6.3 ) days with IFN - alfa ( n = 196 ) . - pubmed
In group 2 , however , there was a significant decrease in post - PCNL 99m - Tc DMSA uptake 2 ( -2.4 + -0.3 % , from 50.1 + -13.5 % to 47.7 + -13.8 % ; P=0.001 ; 95 % CI : 1.13 - 3.66 ) . - pubmed
As a regulator of PD , PDLP5 was also essential for conferring enhanced innate immunity against bacterial pathogens in a salicylic acid - dependent manner . - pubmed
This effect was almost completely abolished by glibenclamide , a blocker of the ATP - sensitive potassium channel , but the cardioprotective activity of SRG was not influenced by the phosphatidylinositol 3 - kinase ( PI3 K ) inhibitor LY294002 . - pubmed
SRG also significantly reduced the Na ( 2 ) S ( 2 ) O ( 4 )-induced increase in intracellular calcium , as shown by Fluo-3 / AM probes with flow cytometry . - pubmed
Moreover , SRG significantly reduced the level of cardiac Troponin I ( cTnI ) in the serum , which suggests that cTnI , a protein component of the troponin regulatory complex involved in cardiac contractility , contributes to the SRG - mediated recovery of cardiac systolic function . - pubmed
SRG significantly attenuated myocardial ischemic injury by improving cardiac systole function , partly by reducing cTnI secretion and improving cardiac diastolic function . - pubmed
Also , SRG attenuated the Ca ( 2 + ) overload in cardiomyocytes and modulated the K(ATP ) , but not PI3 K , signaling pathway ; taken together , these mechanisms synergistically reduced infarct size . - pubmed
In order to investigate titanium surfaces , SEM , AFM and EDS analyses were carried out . - pubmed
Transmission Electron Microscopy ( TEM ) was utilized for Al / CNT agglomerated indicating that the ink paper based on nanocomposite shows good performance as a thermo - piezoresistive sensor . - pubmed
We segmented the anatomical regions of six different muscles from the lower leg , namely the gastrocnemius [ lateral ( GL ) and medial ( GM ) ] , the tibialis [ anterior ( TA ) and posterior ( TP ) ] , the soleus ( S ) and the peroneus ( P ) and measured the local PCr resynthesis rate constants . - pubmed
During the same examination , we also acquired unlocalized ( 31P - MRS data at a temporal resolution of 6 s. At 3 T , the PCr resynthesis rate constants were measured at 25.4 + - 3.7 s [ n = 4 , mean + - standard deviation ( SD ) ] using the MRS method and 25.6 + - 4.4 s using the MRI method . - pubmed
The successful implementation of our 3D - method suggests that imaging is possible at both fields with a relatively high spatial resolution ( voxel size : 4.2 mL at 3 T and 1.6 mL at 7 T ) using volume coils and that local PCr resynthesis rates can be obtained in a single measurement . - pubmed
Maternal and foetal triglycerides , total cholesterol , LDL cholesterol and HDL cholesterol were measured . - pubmed
Tyr(52 ) to Trp ) enthalpically contributed for overcoming the energetic barrier to the antigen - antibody interaction , which was a major hurdle for the in vitro affinity maturation of h528 . - pubmed
Dystrophin - positive cardiomyocytes and increased proliferation of endogenous Nestin(+ ) cardiac stem cells were detected in ADM - injected heart . - pubmed
No dystrophin protein , increase in CD31 expression , or increase in Nestin(+ ) cell proliferation was detected following ADM injection in aged mdx heart . - pubmed
Ribulose-1,5 - bisphosphate carboxylase / oxygenase ( RuBisCO ) is the most abundant protein on the planet and in addition to its central role in photosynthesis it is thought to function as a nitrogen ( N)-storage protein and a potential source of N for defense biosynthesis in plants . - pubmed
The decrease of CSAR caused by IMD was prevented by pretreatment with AM receptor antagonist AM22 - 52 , but not CGRP receptor antagonist CGRP8 - 37 . - pubmed
PVN superoxide anions involve in the effect of IMD on attenuating Ang II - induced CSAR response . - pubmed
Schistosome tegumental ecto - apyrase ( SmATPDase1 ) degrades exogenous pro - inflammatory and pro - thrombotic nucleotides . - pubmed
We find that only SmATPDase1 - suppressed parasites are significantly impaired in their ability to degrade these nucleotides . - pubmed
The enzyme is a true apyrase ; SmATPDase1 degrades ATP and ADP in a cation dependent manner . - pubmed
The Km of SmATPDase1 for ATP is 0.4 + - 0.02 mM and for ADP , 0.252 + - 0.02 mM. - pubmed
The results confirm the role of tegumental SmATPDase1 in the degradation of the exogenous pro - inflammatory and pro - thrombotic nucleotides ATP and ADP by live intravascular stages of the parasite . - pubmed
ZNF165 protein expression was demonstrated on archival formalin - fixed , paraffin - embedded ( FFPE ) bladder tissues using IHC and nuclear staining pattern was detected . - pubmed
These laboratories have analysed during the 3 cycles , 2 levels ( medium and high ) of serum concentration of urea , glucose , creatinine and serum aminotransferases . - pubmed
Angiotensin - converting enzyme ( ACE ) inhibitors and angiotensin II ( AT II ) receptor blockers ( ARBs ) are widely used antihypertensives with well - recognized renoprotective and cardioprotective effects . - pubmed
Highly efficient usage of the hydrothermal technique through the one - pot method to construct four Keggin - based compounds containing pendent ligands . - pubmed
Ginsenoside Ro ( Ro ) , an oleanolic acid - type ginsenoside , exhibited suppressive activities on reactive oxygen species ( ROS ) and matrix metalloproteinase-2 ( MMP-2 ) elevation in UV - B - irradiated fibroblasts . - pubmed
Perfusion variables included PI , arterial lactate level , central venous oxygen saturation , and the difference between central venous carbon dioxide and arterial carbon dioxide pressures , and were recorded before resuscitation and 6 h thereafter . - pubmed
Influence of weight reduction on blood levels of C - reactive protein , tumor necrosis factor - alpha , interleukin-6 , and oxylipins in obese subjects . - pubmed
Plasma Levels of free ( unesterified ) hydroxy , epoxy , and dihydroxy FAs as well as several prostanoids were analyzed in plasma by means of LC - MS - based targeted metabolomics . - pubmed
For most oxylipins no differences were found between the low and high hsCRP groups before and after weight reduction . - pubmed
OBJECTIVE : To evaluate the safety and efficacy of the oral selective JAK1 inhibitor INCB039110 in stable , chronic plaque psoriasis . - pubmed
Therefore we compared the predictive value of the neonatal ( test I ) versus the 48 - h BH4 loading test ( test II ) and long - term BH4 responsiveness . - pubmed
Hemoglobin , xanthine oxidoreductase and carbonic anhydrase ( CA ) have been reported to convert nitrite to NO . - pubmed
The chemical reaction of NO2 (- ) with CO2 and its modulation by CA isoforms may play important roles in the transport of nitrite in red blood cells , the kidney and other cells and organs . - pubmed
Two genes ( pta and poxB ) in the competitive pathways were deleted from this microorganism to inhibit pyruvate consumption . - pubmed
RESULTS : The levels of total cholesterol ( TC ) , high - density lipoprotein cholesterol ( HDL - C ) , low - density lipoprotein cholesterol ( LDL - C ) , tumor necrosis factor ( TNF ) , interleukin-6 ( IL - 6 ) , high - sensitivity C - reactive protein ( hs - CRP ) and left atrial diameter ( LAD ) all differed significantly between the two groups ( P<0.05 ) . - pubmed
The kidneys were used for lipid peroxidation ( LP ) and reactive oxygen species ( ROS ) quantifications . - pubmed
The canola oil - supplemented diet decreased plasmatic concentrations of free fatty acids , triglycerides , and cholesterol ; showed improved osmolarity , water clearances , and creatinine depuration ; and had decreased LP and ROS . - pubmed
We found that both the CpG / GC dense DNA content and the repetitive nature of DUX4p arrays are important for their repressive ability . - pubmed
Using standardized scores for SCZ and BPD scaled to a unit increase doubling the risk of the corresponding disorder , the odds ratios for alcohol and substance use disorders range from 1.19 to 1.31 for the SCZ - PRS , and from 1.07 to 1.29 for the BPD - PRS . - pubmed
In multivariate linear regression analysis , diastolic blood pressure ( beta = -0.129 , P = 0.024 ) and high - density lipoprotein cholesterol ( HDL - C ) ( beta = 0.127 , P = 0.036 ) were related to serum RNase - L. - pubmed
PN regimens are contaminated with Mn in amounts likely to meet requirements . - pubmed
Consequently , it is debated whether PN should be routinely supplemented with Mn . - pubmed
The metabolic flux to acetic acid in phosphoketolase pathway was re - directed to pentose phosphate pathway by substituting the endogenous phosphoketolase gene ( pkt ) with the heterologous transketolase ( tkt ) and transaldolase ( tal ) genes . - pubmed
BACKGROUND / AIM : We have previously reported that caffeine ( CAF ) can enhance chemotherapy efficacy of bone and soft - tissue sarcoma established cell lines via cell - cycle perturbation . - pubmed
We subsequently tested the combination of valproic acid ( VPA ) , a histone deacetylase ( HDAC ) inhibitor , with caffeine on established human osteosarcoma cells in vitro . - pubmed
Dominance of the hypothalamus - pituitary - adrenal axis over the renin - angiotensin - aldosterone system is a risk factor for decreased insulin secretion . - pubmed
How the association between the hypothalamus - pituitary - adrenal ( HPA ) axis and the renin - angiotensin - aldosterone system ( RAAS ) affects glucose metabolism were not well examined in a general population . - pubmed
Principal component ( PC ) analysis identified two PCs : PC1 represented levels of the HPA axis ( serum cortisol ) and the RAAS ( plasma aldosterone ) as a whole , and PC2 represented the HPA axis relative to the RAAS ( HPA axis dominance ) . - pubmed
We examined the association between these PCs and glucose metabolism using homeostasis model assessment indices of reduced insulin sensitivity ( HOMA - R ) and secretion ( HOMA - beta ) . - pubmed
AIM : To compare the efficacy and safety of a single loading low dose MgSo4 regimen with the Pritchard 's regimen . - pubmed
Iodinated CT contrast agents are likely safer than gadolinium - based MRI contrast agents because of gadolinium accumulation in the amniotic fluid and fetal tissue , although no harmful effects of tissue gadolinium accumulation are known . - pubmed
We previously demonstrated an active guanylate cyclase ( GC ) catalytic center in the brassinosteroid insensitive receptor ( AtBRI1 ) within an active intracellular kinase domain resulting in dual enzymatic activity . - pubmed
This may be through a cGMP - AtBRI1 complex forming that may induce a negative feedback mechanism leading to desensitisation of the receptor , regulated through the cGMP production pathway . - pubmed
We further argue that the comparatively low but highly localized cGMP generated by the GC in response to a ligand is sufficient to modulate the kinase activity . - pubmed
MALDI - MS analysis along with NMR spectroscopic studies of a model reaction of hydroxy - phenanthriplatin with acetate establish the formation of stable covalent adducts . - pubmed
Electron transfer dissociation MS / MS fragmentation , a technique that can prevent mass - condition - vulnerable modification of proteins , reveals that Glu97 preferentially participates over Glu106 in covalent bond formation to the platinum center . - pubmed
The Fc conformation of the FcRn - binding sites was similar for wild - type and all mutants of hu3S193 Fc , which suggests that FcRn interactions were directly affected by the amino acid substitutions . - pubmed
The analyte and tramadol(IS ) were extracted from plasma by protein precipitation with methanol , and chromatographied on a XDB C(18)(50 mm x 4.6 mm , 1.8 mum ) column using a mobile phase of methanol and 5 mmol L(-1 ) ammonium acetate(0.01 % ammonia ) . - pubmed
HPQ - PDG is able to detect GGT activity with high spatial resolution and good signal - stability . - pubmed
In this study , identification and preparation of two recombinant sulfatases able to catalyze the cleavage of sulfate groups from fragments of fucoidan molecules is described for the first time . - pubmed
Detailed specificity and catalytic features of sulfatases were determined using various sulfated fucooligosaccharides . - pubmed
Development of CDX-1140 , an agonist CD40 antibody for cancer immunotherapy . - pubmed
CDX-1140 binds outside of the CD40L binding site , and addition of recombinant CD40L greatly enhances DC and B activation by CDX-1140 , suggesting that CDX-1140 may act synergistically with naturally expressed CD40L. - pubmed
Our outcomes of interest were daily intakes of vitamin C , magnesium , dietary fiber , and potassium as estimated by the Block Fruit - Vegetable - Fiber Screener and kidney disease defined as estimated glomerular filtration rate & lt;60 mL / min per 1.73 m2 or urinary albumin - to - creatinine ratio & gt;=30 mg / g. SETTING AND SUBJECTS : Baseline data from the Achieving Blood Pressure Control Together study , a randomized controlled trial of 159 African Americans ( 117 females ) with uncontrolled hypertension , were obtained . - pubmed
MAIN OUTCOME MEASURES : Our outcomes of interest were daily intakes of vitamin C , magnesium , dietary fiber , and potassium as estimated by the Block Fruit - Vegetable - Fiber Screener and kidney disease defined as estimated glomerular filtration rate & lt;60 mL / min per 1.73 m2 or urinary albumin - to - creatinine ratio & gt;=30 mg / g. RESULTS : Overall , compared to Institute of Medicine recommendations , participants had lower intakes of magnesium , fiber , and potassium but higher vitamin C intakes . - pubmed
Below - median intake was significantly associated with albumin - to - creatinine ratio & gt;=30 ( adjusted odds ratio [ 95 % confidence interval ] : 3.4 [ 1.5 , 7.8 ] for vitamin C ; 3.6 [ 1.6 , 8.4 ] for magnesium ; 2.9 [ 1.3 , 6.5 ] for fiber ; 3.6 [ 1.6 , 8.4 ] for potassium ) , but not with estimated glomerular filtration rate & lt;60 . - pubmed
After positive poling , the depolarization field across the whole volume of BFO film drove electrons into the electrolyte solution , inducing a significant anodic shift , Vop of 1.01 V vs. RHE , together with a significantly enhanced photocurrent density of -91 muA / cm2 at 0 V vs. RHE under 100 mW / cm2 illumination . - pubmed
Herein , an integrated Photocatalytic Chitosan - La3 + - Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR , XRD , TGA , EDX and SEM analysis . - pubmed
Arginine deiminase ( ADI ) is a therapeutic protein for cancer therapy of arginine - auxotrophic tumors . - pubmed
Four Arg residues on the surface of PpADI M31 were selected through three criteria and subsequently substituted to Lys , aiming for providing primary amines for PEGylation . - pubmed
The strain S17 produced 297.85 mg / L of higher alcohols at 20 C , and the production did not increase at 25 C , reaching about 297.43 mg / L. Metabolite analysis and transcriptome sequencing showed that the metabolic pathways of branched - chain amino acids , pyruvate , phenylalanine , and proline were the decisive factors that affected the formation of higher alcohols . - pubmed
The total production of higher alcohols by the mutants Deltatir1 and Deltagap1 was reduced by 23.5 and 19.66 % compared with the parent strain S17 , respectively . - pubmed
In this study , thin - layered black phosphorus ( TLBP ) was used as a unique supporting substrate for the deposition of metal nanoparticles ( MNPs , M = Pt , Ag , Au ) , and the resulting M - TLBP nanocomposites were found to exhibit apparent ORR activity that was readily manipulated by interfacial charge transfer from TLBP to MNPs . - pubmed
The ultrasonic sensitivity of a sensor ( T : EVA and R : EVA ) significantly enhanced the acoustic impedance in a methane gas space as pressure increases . - pubmed
Here , we systematically knocked out TJ components by genome editing and show that while ZO-1 / ZO-2 - deficient cells lacked TJ structures and epithelial barriers , claudin - deficient cells lacked TJ strands and an electrolyte permeability barrier but formed membrane appositions and a macromolecule permeability barrier . - pubmed
Compared with intracavernous ( IC ) injection of phosphate - buffered saline after artery injury , of note , we observed that both mesenchymal stem cells ( MSCs ) and MSC - Exos through IC injection could improve the erectile function to varying degrees . - pubmed
More specifically , IC injection MSC - Exos could promote cavernous sinus endothelial formation , reduce the organization oxidative stress damage , and improve the nitric oxide synthase and smooth muscle content in the corpus cavernosum . - pubmed
Selected gene candidates from these groups were verified by qRT - PCR as targets of EVNorm in CCl4 - injured livers . - pubmed
Additionally , EVNorm administration resulted in reduced activation of p53 , a predicted upstream regulator of 40 % of the genes for which expression was altered by EVNorm following CCl4 liver injury . - pubmed
Currently , the available treatment options are very restricted and colistin - based therapies are last - line treatments of these infections , even though colistin resistant ( COLR ) Ab have rarely been isolated yet . - pubmed
CNP3 was expressed in several endocrine cells : prolactin - producing cells ( pituitary ) , glucagon - producing cells ( pancreas ) , and cardiomyocytes ( heart ) . - pubmed
We also showed the negative correlation between CNP3 / prolactin expression in the pituitary and plasma Cl- concentration , but not for plasma Na+ concentration . - pubmed
In this work , we used polyacrylamide ( PA ) gels with varying stiffness ( 6.5 - 92.5 kPa ) as the substrate to construct a mechanical microenvironment for cardiomyocytes . - pubmed
The extensive surface remodeling induced by the deposition of the apatite - like layer lead to a 3 - fold increase in surface area , a 5 - fold increase in the roughness , and 4 - fold increase in the hardness of the PBS - immersed scaffolds when compared to the as - printed counterpart . - pubmed
Hepcidin is a key molecule that regulates iron metabolism in the body . - pubmed
The results showed that WBPPS-1 , WBPPS-2 and WBPPS-3 were composed of mannose , rhamnose , galacturonic acid , glucose , galactose , xylose and arabinose with different ratio and their average molecular weights were 1016.3 , 878.7 and 901.6 KDa , respectively . - pubmed
An ab initio chemical kinetics study of the reactions of methyl butanoate ( MB ) with hydroperoxyl radical ( HO2 ) is presented in this paper . - pubmed
The transition state theory ( TST ) is used to calculate the high - pressure limit rate constants of the hydrogen abstraction reactions over a board range of temperature ( 500 - 2000 K ) . - pubmed
Risk assessment of osteoradionecrosis associated with periodontitis using 18F - FDG PET / CT . - pubmed
Different with majority of reported hybrid photochromic compounds driven by photochromic active units , the photochromism in 1 and 2 is derived from the electron transfer ( ET ) between phosphite and non - photochromic triimidazole - derivative ligand TIPA . - pubmed
Association of time in range with hemoglobin A1c , glycated albumin , and 1,5 - anhydro - D - glucitol . - pubmed
AIMS : Hemoglobin A1c ( HbA1c ) , glycated albumin ( GA ) , and 1,5 - anhydro - D - glucitol ( 1,5 - AG ) are used as indicators of glycemic control , whereas continuous glucose monitoring ( CGM ) is used to assess daily glucose profiles . - pubmed
RESULTS : In T1DM and T2DM , HbA1c and GA were negatively correlated with TIR , whereas 1,5 - AG was positively correlated with TIR . - pubmed
The amino lipid choice ( cationic - DOTAP and DDAB ; ionisable - MC3 ) , buffer choice ( citrate buffer pH 6 or TRIS pH 7.4 ) and type of nucleic acid payload ( PolyA , ssDNA or mRNA ) have also been shown to have an impact on the characteristics of these LNPs . - pubmed
Many APOBEC cytidine deaminase members are known to induce ' off - target ' cytidine deaminations in 5'TC motifs in genomic DNA that contribute to cancer evolution . - pubmed
BRET data suggested substantial inhibition of CYP1A2 - mediated 7 - ethoxyresorufin de - ethylation in the presence of HO-1 , whereas its activity was actually stimulated at subsaturating POR . - pubmed
In contrast , HO-1 - mediated heme metabolism was inhibited at subsaturating POR . - pubmed
Regarding alcohol use , at nine months post detox , the average units of alcohol consumption by participants was 18.7 units per week compared to 130.6 units per week before the detox . - pubmed
Two labeled products were formed ; one was a glycogen - like polysaccharide and the other was a glycoprotein with glucosyl chains bound to protein through an acid - labile bond . - pubmed
After mild treatment of the glucoprotein with acid , the product liberated from the protein behaved as a mixture of malto - oligosaccharides and alpha-1,4 - glucan with branches . - pubmed
The carbohydrate moiety of the glucoprotein seemed to be released upon prolonged incubation with the enzyme preparation . - pubmed
5 - HT antagonists inhibit neuroleptic and morphine antagonism of the hyperactivity induced by DA from the nucleus accumbens [ proceedings ] . - pubmed
The hydrophobic - rich NH2 - terminal 34 amino acids of a tetracycline resistance determinant ( TetC ) were fused to the COOH - terminal 240 amino acids of the hemolysin transporter , HlyB , which contains a putative ATP - binding domain . - pubmed
Severe head injury often causes an increase in intracranial pressure ( ICP ) and decreases in cerebral blood flow ( CBF ) and cerebral oxygen delivery ( CO2del ) . - pubmed
The role of carbohydrates in the antigenic properties of influenza C viral glycoproteins . - pubmed
The antigenic properties of influenza C viral glycoprotein gp88 were compared with those of its nonglycosylated counterpart T76 synthesized in infected cells treated with tunicamycin . - pubmed
It is unlikely , however , that the antigenic determinant recognized by Q-5 is carbohydrate moiety since the ability of the antibody to bind to gp88 varied depending on the virus strain , and trypsin - treatment of gp88 eliminated its reactivity with Q-5 . - pubmed
Gel electrophoretic analysis under nonreducing conditions showed that T76 underwent the formation of disulfide - linked multimers in the absence of reducing agent while gp88 behaved as monomers , suggesting that glycosylation is required for gp88 molecules to attain an appropriate conformation . - pubmed
Energy requirements were found to be suppressed , protein metabolism impaired , loss of nitrogenous products increased , fluid and electrolyte excretion accelerated , vitamin elimination was enhanced , corticosteroid blood concentration decreased , and hemoglobin and cell counts increased . - pubmed
The limiting factor was found to be the activity of a microsomal deamidase ( pyrazinoic acid formation from pyrazinamide and 5 - hydroxy - pyrazinoic acid formation from 5 - hydroxy - pyrazinamide ) . - pubmed
In contrast , oxidation by xanthine oxidase occurred very rapidly ( 5 - hydroxy - pyrazinamide formation and pyrazinoic acid catabolism to 5 - hydroxy - pyrazinoic acid ) . - pubmed
Different factors that give the maximum iodination of proteins by iodogen method have been evaluated . - pubmed
The quantity of iodogen was varied from 2 to 20 micrograms ; pH from 3 to 9 ; the quantity of bovine serum albumin ( BSA ) from 25 micrograms to 2 mg ; the 131I activity from 200 microCi to 5 mCi and the reaction time from 2 to 30 min . - pubmed
The maximum iodination of proteins is achieved with iodogen - to - iodide molar ratio of 100 - 200 and iodogen - to - protein molar ratio of 1 - 2 at pH 6 - 8 for 10 - 15 min at room temperature . - pubmed
Lipid levels did not change , except that low - density lipoprotein levels decreased from 148 to 127 mg / dl with prazosin . - pubmed
Chiral nature of covalent methylphosphonyl conjugates of acetylcholinesterase . - pubmed
This paper examines the chiral nature of the covalent conjugates formed upon reaction of acetylcholinesterase ( AchE ) with enantiomeric cycloheptyl , isopropyl , and 3,3 - dimethylbutyl methylphosphonyl thiocholines . - pubmed
Equilibrium titrations with decidium , a fluorescent bisquaternary competitive inhibitor of AchE , provided an index of aging and enantiomeric configuration of the conjugates independent of enzyme activity . - pubmed
These studies indicate that the active center of AchE comprises at least two kinetically distinct environments separate from the esteratic region but located within 5 A of the nucleophilic serine and differing in dipolar characteristics that promote charge separation and general acid catalysis . - pubmed
Treatment of prolactin or bromocriptine failed to induce any change in esterified and free cholesterol content of the testis . - pubmed
On the other hand , bromocriptine treatment resulted in an increase in the activity of Mg2 + - ATPase and phospholipid : DNA ratio of the testis with a concomitant decrease in its DNA content . - pubmed
A reevaluation of the amino acid sequence of human follitropin beta - subunit . - pubmed
Toluene ( 1 g / kg , lh ) did not alter total brain microsomal phospholipid ( PL ) or cholesterol ( CL ) content . - pubmed
The PL / CL ratio , an indirect index of membrane fluidity , did not change , suggesting that toluene did not affect membrane fluidity . - pubmed
Dose - response and time - course studies showed that toluene maximally decreased synaptosomal PL after 1 g / kg , 1 h. The dose - response and time - course studies also showed that the toluene - induced decreases in PL were a result of specific decreases in phosphatidylethanolamine ( PE ) . - pubmed
Since PE was decreased , we assessed whether toluene altered synaptosomal membrane function by investigating phospholipid methylation , a reaction which uses PE as its initial substrate . - pubmed
Active site - directed irreversible inhibition of glutathione S - transferases by the glutathione conjugate of tetrachloro-1,4 - benzoquinone . - pubmed
The derivative bound to the enzyme still possesses a quinone structure and is able to react with thiol - containing compounds . - pubmed
Reduction of the enzyme - bound quinone abolishes its reactivity but does not decrease the inhibition . - pubmed
At 0 degrees C , the glutathione conjugate of tetrachloro-1,4 - benzoquinone inhibits the glutathione S - transferases at a much higher rate than the corresponding beta - mercaptoethanol conjugate , indicating a distinct targetting effect of the glutathione moiety . - pubmed
However , the parent compound , tetrachloro-1,4 - benzoquinone , also has a considerable affinity for the enzymes . - pubmed
Tumor necrosis factor augments the immunogenicity and the production of L - ornithine by peritoneal macrophages . - pubmed
The release of ornithine by macrophages and its correlation with their immunogenicity after treatment with recombinant human tumor necrosis factor - alpha ( TNF ) were analyzed . - pubmed
TNF was found to augment the capacity of peritoneal macrophages to convert 14C - labeled arginine into L - ornithine and to release L - ornithine into the culture medium . - pubmed
T1 measured in a tubule suspension ( 22 % of exchangeable water in the intracellular compartment ) containing 20 mM extracellular MnCl2 was biexponential with time constants 1.8 + /- 0.1 ms and 8.3 + /- 0.2 ms ( mean + /- SD , n = 8 , 37 degrees C , 10 MHz ) . - pubmed
The model predicted that the slow T1 was relatively insensitive to apical membrane Pd because of low luminal / cell volume ratio . - pubmed
Individual cells in heterogeneous populations can be tested for cell surface antigens using monoclonal antibodies conjugated to fluorescein - containing microspheres and subsequently stained for myeloperoxidase , Sudan black B , chloroacetate esterase , or alpha naphthyl butyrate . - pubmed
Collagen content was measured in 38 needle liver biopsies ( 8 steatosis , 8 chronic hepatitis , 7 fibrosis and 15 cirrhosis ) by a new colorimetric method based on the selective capacity of Sirius red and Fast green to bind to collagen and noncollagenous proteins , respectively . - pubmed
Globin from hemoglobin A and hemes with altered 2,4 - substituents gave reconstituted hemoglobins that autooxidize at rates that decrease with the electron - withdrawing power of the substituent : acetyl greater than vinyl greater than hydrogen greater than ethyl . - pubmed
Acetylcholine release from growth cones detected with patches of acetylcholine receptor - rich membranes . - pubmed
To examine the development of ACh release we have used isolated patches of acetylcholine receptor(AChR)-rich membrane as sensitive detectors of ACh . - pubmed
Cytochrome P-450 oxidation of alkanes originating as scission products during lipid peroxidation . - pubmed
Administration of drugs which inhibit or induce cytochrome P-450 indicated that a particular isoenzyme might be involved in the oxidation of small alkanes . - pubmed
CCl4 destroys cytochrome P-450 dose dependently , so that it loses its capacity to oxidize pentane , whereas paracetamol does not inactivate the mono - oxygenase . - pubmed
Monitoring the elimination rate of injected pentane is recommended as a reliable non - invasive procedure for testing the functional state of hepatic cytochrome P-450 . - pubmed
These findings demonstrate that the formation of GSSQ is catalyzed by a GSSG specific enzyme which is supposed to be thioltransferase ( Glutathione : Disulfide Oxidoreductase ) . - pubmed
A specific inhibitor of gamma - glutamyltranspeptidase , anthglutin , inhibited the hydrolysis . - pubmed
The most sensitive UV detection of trace amounts of CT was achieved with tetrabutylammonium phosphate buffer ( TBAP ) . - pubmed
We recommend for RP - HPLC of CT a C18 - bonded silica column and elution with a 20 - min linear gradient of methanol - water ( 20:80 to 80:20 , v / v ) containing 0.005 M TBAP . - pubmed
Combined with appropriate extraction procedures , such as silica adsorption or immuno - adsorbant chromatography , this paired - ion RP - HPLC method can be an important aid in achieving more accurate and extensive information about CT moieties in biological samples . - pubmed
Phosphoamino acid analysis of the autophosphorylated kinase shows that ADK1 phosphorylates serine , threonine , and tyrosine . - pubmed
Using poly ( Glu / Tyr ) as a substrate , we confirm that ADK1 is capable of phosphorylating tyrosine residues . - pubmed
METHODS AND RESULTS : To evaluate the presence of reinnervation , we measured the power of RR - LF and RR - HF in 26 heart transplant recipients and 16 control subjects before and during sinusoidal neck suction at 0.1 Hz and 0.20 Hz ( similar to but distinct from that of controlled respiration , 0.25 Hz ) and before and during administration of atropine or beta - blocker ( esmolol hydrochloride ) by spectral analysis . - pubmed
The organisation of the cytoskeleton was determined by double fluorescence labeling with antibody anti - vimentin , anti - vinculin , anti - tubulin , and with rhodamine - labeled phalloidin . - pubmed
The only CpG island found in this region was within the N - myc gene . - pubmed
LAZ3 is transcribed as a 3.8 kb message predominantly in normal adult skeletal muscle and in several NHL carrying 3q27 chromosomal defects . - pubmed
Gossypol ( GP ) , a naturally occurring toxin , inhibits the growth of various carcinoma cells . - pubmed
Decreased [ 3H ] thymidine incorporation was observed in post - confluent cultures of both cell types relative to pre - confluent counterparts . - pubmed
Light production was dependent on phage infection , expression of the luciferase gene , and the level of cellular adenosine triphosphate . - pubmed
Disintegrins , a group of Arg - Gly - Asp ( RGD)-containing peptides from viper venom , are potential anti - adhesion agents that interfere with integrin - ECM binding . - pubmed
The cytotoxic effect of disintegrins was examined with a cell proliferation assay using the alamar blue method . - pubmed
Disintegrins , echistatin , and flavoridin inhibited RPE cell attachment to the ECM . - pubmed
The potency of disintegrins was 150 to 300 times higher than that of Gly - Arg - Gly - Asp - Ser ( GRGDS ) peptide . - pubmed
In the in vivo experiment , echistatin ( 50 microgram / ml ) or flavoridin ( 80 microgram / ml ) significantly inhibited RPE cell - induced tractional retinal detachment compared with the control group at week 2 ( P< ; 0.05 ) and week 4 ( P< ; 0.01 ) after surgery . - pubmed
We propose that nedocromil sodium and cromolyn sodium can affect water transport into and out of the epithelial cell by an action on chloride ion channels . - pubmed
GK mRNA was induced by insulin maximally under venous O2 partial pressure ( pO2 ) and only half - maximally under arterial pO2 . - pubmed
CoCl2 and desferrioxamine mimicked venous pO2 and enhanced the insulin - dependent induction of GK mRNA under arterial pO2 . - pubmed
H2O2 mimicked arterial pO2 and reduced insulin - induced GK mRNA under venous pO2 to the lower arterial levels . - pubmed
Thus the zonal O2 gradient in liver seems to have a key role in the heterogenous expression of the GK gene . - pubmed
Since the presence of the TNFa6 allele correlates with low TNF alpha production in response to lipopolysaccharide , it could be speculated that DR2 - negative MS patients have an increased risk of being high TNF alpha producers in response to exogenous stimuli . - pubmed
Increased expression of c - jun , but not retinoic acid receptor beta , is associated with F9 teratocarcinoma stem cell differentiation induced by polyamine depletion . - pubmed
alpha - Difluoromethylornithine ( DFMO ) , an enzyme - activated irreversible inhibitor of ornithine decarboxylase , and all - trans - retinoic acid ( RA ) are known to induce F9 teratocarcinoma stem cell differentiation . - pubmed
Thus , retinoic acid receptor ( RAR ) alpha mRNA is weakly expressed during DFMO treatment , but strongly induced during an early phase of RA treatment . - pubmed
RAR gamma mRNA is relatively strongly expressed in untreated control cells and remains at approximately the same level during DFMO - induced differentiation . - pubmed
Hevin , a gene closely related to the extracellular matrix protein SPARC , is an acidic cysteine - rich glycoprotein shown to be important for the adhesion and trafficking of cells through the endothelium . - pubmed
In situ hybridization with an 35S - labeled antisense riboprobe demonstrated the loss of Hevin expression in metastatic prostate carcinoma . - pubmed
Mo accumulated in all organs including brain and pituitary ( P & lt ; 0.02 ) in all TTM trial groups and was retained after cessation of treatment , except in liver , kidney and skeletal muscle . - pubmed
Brain Cu v. Mo concentrations showed a strongly positive correlation ( r. 0.7 ) in the high - Cu Ronaldsay group 7 months after TTM treatment . - pubmed
In addition TTM may redistribute some displaced excess liver Cu ( Cu - TTM ) to the brain . - pubmed
Activation of postsynaptic D-1 and D-2 dopamine ( DA ) receptors with apomorphine administered 30 min before stress attenuated stress - induced immunosuppression as well . - pubmed
RESULTS : The Vmean and heart rate increased significantly with increasing concentrations of desflurane ( Vmean from 68 + /- 27 to 106 + /- 30 cm.s-1 and heart rate from 109 + /- 17 to 136 + /- 15 b.min-1 between T1 and T7 ) . - pubmed
Calcium , phosphate , 25 - hydroxyvitamin D , and osteocalcin increased significantly from midpregnancy to weaning whereas 1,25 - dihydroxyvitamin D values showed significant decreases . - pubmed
The vitamin supplemented lipopolysaccharide ( LPS ) vaccinated group exhibited greater immune ( both humoral and cell mediated ) responses than its formalin killed ( FK ) and heat killed ( HK ) bacterin vaccinated counterparts . - pubmed
These actions of NOHA were abolished in the presence of exogenous L - ornithine suggesting that a reduction in the intracellular polyamine content might be responsible for the activation of caspase-3 and apoptotic actions of NOHA . - pubmed
In order to further explore this possibility , we used SAM-486A and alpha - difluoromethylornithine ( DFMO ) , which are inhibitors of S - adenosylmethionine decarboxylase ( SAMDC ) , and ornithine decarboxylase ( ODC ) , respectively , either alone or in combination to reduce the intracellular polyamine levels . - pubmed
We then assessed whether a reduction in polyamine levels by these two compounds to a similar degree to that produced by NOHA activated caspase-3 which occurs prior to the onset of apoptosis . - pubmed
We observed that both SAM-486A and DFMO , either alone or in combination , inhibited cell proliferation , induced p21 and arrested cells in the G(0)-G(1 ) phase of the cell cycle but failed to activate caspase-3 as assessed by enzymatic assay of caspase-3 , western blot analysis of the proteolytic cleavage of caspase-3 protein as well as TUNEL assay . - pubmed
Furthermore , pre - incubation of the cells with SAM-486A and DFMO for 4 days , either alone or in combination significantly inhibited the activation of caspase-3 and apoptosis by NOHA when compared with that observed with cells treated with NOHA alone . - pubmed
Our results , therefore , indicate that the activation of caspase-3 and apoptosis observed with NOHA can not be solely explained by a reduction in intracellular polyamine levels and that other mechanisms need to be also considered . - pubmed
The HLA class I gene HFE seemingly no longer participates in immunity because it has lost its ability to bind peptides and it has acquired the ability to form complex with the receptor for iron - binding transferrin by regulating iron uptake by intestinal cells . - pubmed
The usefulness of many beta - lactams , including the third - generation cephalosporins , has been threatened in recent years by the dissemination of beta - lactamase - mediated resistance . - pubmed
Due to the unique arrangement of the moieties around the carbapenem beta - lactam ring , these agents possess unrivaled stability to almost all serine beta - lactamases , including the mutant extended - spectrum types produced by Enterobacteriaceae which hydrolyze third - generation cephalosporins . - pubmed
The presence of CO , however , further increased both pulmonary vascular resistance ( PVR ) and the number of small muscular vessels compared with HH alone . - pubmed
CO + HH also increased vascular PCNA and nuclear ssDNA expression compared with hypoxia , suggesting accelerated cell turnover . - pubmed
CO in hypoxia downregulated sm - alpha - actin and strongly upregulated beta - actin . - pubmed
CO also increased lung HO activity and HO-1 mRNA and protein expression in small pulmonary arteries during hypoxia . - pubmed
These data indicate an overall propensity of CO in HH to promote vascular remodeling and increase PVR in vivo . - pubmed
As a prodrug leflunomide is completely converted to its active metabolite A 77 1726 ( M1 ) which blocks the dihydroorotate dehydrogenase , a key enzyme of the pyrimidine de novo synthesis . - pubmed
This study investigated the contribution of the entorhinal cortex in this reinnervation by comparing the distribution of the entorhinal axons in the CA1 subfield between the intact hippocampus and the CA3 - lesioned hippocampus at 3 months after administration of kainic acid . - pubmed
However , after kainic acid - induced CA3 lesion , the density of entorhinal axons increased significantly in the CA1 stratum radiatum ( 375 % of the intact hippocampus ) , as a large number of axons emanating from the entorhinal fiber plexus in the stratum lacunosum moleculare invaded the stratum radiatum . - pubmed
BFA induced a rapid regression of the Golgi stack into rudimentary Golgi clusters composed of tubulovesicules , in parallel with a redistribution of the Golgi - resident proteins and a coat protein ( beta - COP ) into the region of the rough endoplasmic reticulum ( rER ) or cytosol . - pubmed
[ Pharmacokinetic / pharmacodynamic analysis of anti - hyperprolactinemic effect of terguride based on dopamine D2 receptor occupancy ] . - pubmed
Terguride has been widely used for the treatment of hyperprolactinemia via partial agonistic action on dopamine D2 receptors in the pituitary . - pubmed
The present study analyzed retrospectively the dopamine D2 receptor binding occupancy ( phi ) of terguride . - pubmed
The average phi value was estimated to be 14.1 % after oral administration of the average / standard therapeutic dose of terguride . - pubmed
Furthermore , we developed a pharmacokinetic / pharmacodynamic model for ascertaining the serum prolactin - lowering effect of terguride , considering both the reversible binding to D2 receptors and the effect on the increase rate in the prolactin level . - pubmed
Thus , conditions in which methanol would not be produced in the headspace - GC method were investigated . - pubmed
melanogaster species subgroup , the histone gene regions as well as 3rd codon position of the coding region showed nearly the same GC contents . - pubmed
These results suggested that the previous conclusion on analysis of the H3 gene regions , the gene family evolution in a concerted fashion , holds true for the whole histone gene repeating unit . - pubmed
Pavlovian fear conditioning regulates Thr286 autophosphorylation of Ca2 +/ calmodulin - dependent protein kinase II at lateral amygdala synapses . - pubmed
Next , we demonstrate that intra - amygdala infusion of a CaMK inhibitor , 1 -[ NO - bis-1,5 - isoquinolinesulfonyl ]- N - methyl - l - tyrosyl-4 - phenylpiperazine , KN-62 , dose - dependently impairs the acquisition , but not the expression , of auditory and contextual fear conditioning . - pubmed
Finally , in electrophysiological experiments , we demonstrate that an NMDA receptor - dependent form of long - term potentiation at thalamic input synapses to the LA is impaired by bath application of KN-62 in vitro . - pubmed
Reducing the number of treatments to twice a week and reducing the UVA dose from 1 MPD to 0.75 or 0.5 MPD per treatment only slightly affected intermediate therapeutic efficacy ( between the second and seventh weeks of therapy ) but had no effect on final clearance rates . - pubmed
During tissue damage , S - alk ( en ) yl cysteine sulfoxides are converted to their respective thiosulfinates or propanethial - S - oxide by the action of the enzyme alliinase ( EC 4.4.1.4 ) . - pubmed
Low - dose STI571 monotherapy was not effective in extra - osseous disease , apparently due to a site - specific failure to up - regulate beta - platelet - derived growth factor receptor expression in prostate cancer cells and associated tumour stroma . - pubmed
Both these effects were blocked by the TK inhibitor tyrphostin-23 , while the MAPK p42 / p44 inhibitor PD-98059 annulled only MAPK p42 / p44 activation . - pubmed
The growth - stimulating effect of 10(-6 ) M NPB and NPW were not affected by either the PKA and PKC inhibitors H-89 and calphostin - C or the MAPK p38 antagonist SB-293580 , but were abolished by both tyrphostin-23 and PD-98059 . - pubmed
Addition of 0.15 g / L MgSO ( 4 ) was the most favorable for levansucrase production . - pubmed
The results indicated that high enzyme concentrations produced increasing amounts of levan , and hence conversion of fructose to levan reached 84 % using 1,000 microg / ml enzyme protein . - pubmed
A sensitive assay for lipase using tetra sulfonatophenyl porphyrin . - pubmed
The coupling of NSAIDs with a NO - donating moiety has led to the birth of a new class of anti - inflammatory drugs , called the COX - inhibiting nitric oxide donators ( CINODs ) . - pubmed
Rate constants for reactions of secondary alkyl radicals with triethylborane and its complexes with water , deuterium oxide , methanol , and THF at ambient temperature were determined by radical clock methods . - pubmed
Cyclization of the 1 - undecyl-5 - hexenyl radical and ring opening of the 1 - cyclobutyldodecyl radical were evaluated as clock reactions . - pubmed
L and D isomers of amino acids were employed as inhibitors to determine the stereo - selectivity of LAT . - pubmed
RESULTS : Retinal uptake of L - Phe was significantly inhibited in presence of a specific LAT inhibitor , 2 - aminobicyclo -[ 2,2,1 ]- heptane-2 - carboxylic acid ( BCH ) , L isomers of large neutral amino acids and LAT1 specific but not by LAT2 specific and charged amino acids . - pubmed
No significant inhibition of L - Ala retinal uptake was observed with LAT substrates . - pubmed
The mean residence time ( MRT ) and area under curve ( AUC ) values of L - Phe following intravitreal administration were significantly increased in the presence of D - methionine , a LAT substrate . - pubmed
Furthermore , based on these studies it can be concluded that LAT is involved in the retinal uptake and intravitreal elimination of L - Phe . - pubmed
Serum proprotein convertase subtilisin kexin type 9 is correlated directly with serum LDL cholesterol . - pubmed
BACKGROUND : Proprotein convertase subtilisin kexin type 9 ( PCSK9 ) is gaining attention as a key regulator of serum LDL - cholesterol ( LDLC ) . - pubmed
RESULTS : Serum concentrations of PCSK9 ranged from 11 to 115 microg / L and were directly correlated with serum concentrations of LDLC ( r = 0.45 , P = 0.001 ) and total cholesterol ( r = 0.50 , P = 0.0003 ) , but not with triglycerides ( r = 0.15 , P = 0.28 ) or HDL cholesterol concentrations ( r = 0.13 , P = 0.36 ) . - pubmed
Forced expression of the alpha7 integrin or use of the mitogen - activated protein kinase kinase 1 inhibitor U0126 in alpha7 integrin - deficient VSMCs suppressed extracellular signal - regulated kinase activation and restored the differentiated phenotype to alpha7 integrin - null cells in a manner dependent on Ras signaling . - pubmed
2 - Methoxyestradiol ( 2 - ME ) is an endogenous metabolite of estradiol with no affinity for estrogen receptors . - pubmed
The goal of this round - robin study is to investigate the capability of a novel solid - phase microextraction - gas chromatographic ( SPME - GC ) method for trace - level EtO residuals analysis : three independent laboratories conducted a guided experiment using this SPME - GC method , in assessing method performance , ruggedness and the feasibility of SPME fibers . - pubmed
SPME is a feasible alternative for quantifying trace - level EtO residuals in sterilized medical devices , thereby lowering the limit of quantification ( LOQ ) by as much as two to three orders of magnitude over the current GC methodology of direct liquid injection . - pubmed
Mutations in different codons causing resistance to isoniazide in the gene of catalase peroxides ( katG ) in Belarusian strains was determined . - pubmed
This indicated that METH induced an increase in the phosphorylation of ERK and JNK , but not of p38 . - pubmed
Moreover , a JNK - specific inhibitor , SP600125 , partially but significantly rescued METH - induced cell death , while PD98059 ( an ERK kinase inhibitor ) and SB203580 ( a p38 inhibitor ) had no protective effect . - pubmed
We also found that vitamin E ( Vit E ) prevented METH - induced JNK phosporylation and SP600125 inhibited METH - induced c - Jun phosphorylation . - pubmed
Furthermore , METH - activated caspase-3 activity was significantly repressed by Vit E and in SP600125 treated cells . - pubmed
We suggest that the oxidative stress - activated JNK signaling pathway is involved in METH - induced cell death . - pubmed
Metal selective fluorescent peptide probes ( dansyl - Cys - X - Gly - His - X - Gly - Glu - NH2 , X = Pro or Gly ) were developed by synthesizing peptides containing His , Cys , and Glu residues with Pro - Gly sequence to stabilize a turn structure and Gly - Gly sequence to adopt a random coil . - pubmed
The probe containing two Gly - Gly sequences exhibited marked selectivity only for Cu2 + over 13 metal ions including competitive transition and Group I and II metal ions under physiological buffer condition . - pubmed
The purified protein showed a single protein band and the molecular weight was about 110kDa on sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( SDS - PAGE ) under reducing conditions . - pubmed
Vitamin D2 and ergosterol were measured by HPLC - MS / MS . - pubmed
Early outcomes of thymoglobulin and basiliximab induction in kidney transplantation : application of statistical approaches to reduce bias in observational comparisons . - pubmed
RESULTS : All statistical approaches demonstrated lower rates of the 6 - month triple endpoint with thymoglobulin compared with basiliximab when steroids were present , with approximately 22 % adjusted , relative reduction by logistic regression analysis and 3 % absolute reductions by matching approaches . - pubmed
CONCLUSIONS : Consistency across statistical approaches suggests superiority of thymoglobulin compared with basiliximab or no antibody induction therapy for 6 - month kidney transplant outcomes in the modern immunosuppression era . - pubmed
Baseline albuterol use , total daily asthma score , and nighttime awakenings were maintained after CIC treatments ( p & gt ; 0.25 ) , but increased after placebo ( p & lt ; or = 0.002 ) ; the difference between CIC80 b.i.d . - pubmed
An NMR study on nickel binding sites in Cap43 protein fragments . - pubmed
NMR spectroscopy was used to study the interaction of Ni ( II ) ions with C - terminal sequence of Cap43 protein where , from Thr341 to Gly360 residue , a T1R2S3R4S5H6T7S8E9G10 ten - amino acid fragment is consecutively repeated three times . - pubmed
The behaviour of ends - blocked Ac - RSRSHTSEG - Am ( pept1 ) , Ac - TRSRSHTSEG - Am ( pept2 ) , and the three repeats Ac - TRSRSHTSEG - TRSRSHTSEG - TRSRSHTSEG - Am ( pept3 ) peptides towards Ni ( II ) ions was examined at different pH values and , for pept3 , at different ligand - to - metal molar ratios . - pubmed
In the present work four new , tyrosine containing HN - derivatives were synthesized ( Y - PAGASRLLLTGEIDLP , peptide I ; Y - PAGASRLLLLTGEIDLP , peptide II ; Y - SALLRSIPAPAGASRLLLTGEIDLP , peptide III ; Y - SALLRSIPAPAGASRLLLLTGEIDLP , peptide IV ) . - pubmed
[ Myoanabolic steroids and selective androgen receptor modulators : mechanism of action and perspectives ] . - pubmed
Interest in anabolic steroids has been renewed in the last decade with the discovery of tissue - selective androgen receptor modulators exhibiting high myotropic and small androgenic activity . - pubmed
An explanation put forward by us in 1982 for the mechanism of the preferential myotropic effect of nandrolone ( 19 - nortestosterone ) exploits the fundamental difference between the 5alpha - reductase concentrations in skeletal muscle and androgenic target tissue . - pubmed
Transcapillary ultrafiltration and fluid absorption rates were assessed using radiolabeled albumin , and free water transport by kinetics of sodium concentration in dialysis fluid . - pubmed
Unexpectedly severe acute radiotherapy side effects are associated with single nucleotide polymorphisms of the melanocortin-1 receptor . - pubmed
Deoxyribonucleic acid sequence variants in the form of single nucleotide polymorphisms ( SNPs ) of MC1R affect melanin expression and are linked to skin phenotype . - pubmed
Diminution of AHR and inflammation was associated with reduced IL-4 , IL-5 , and RANTES production in the BAL fluid at the 30 microg ITE dose , while OVA specific IgE and eotaxin serum levels remained unchanged . - pubmed
CONCLUSION : ITE , which has been reported inhibiting COX-2 and 5 - LOX , reduced allergic airway inflammation and AHR by inhibiting the production of the Th2 cytokines IL-4 and IL-5 , and RANTES . - pubmed
Syndecan-1 - integral membrane heparan sulfate proteoglycan and cathepsins D and K - lysosomal proteases are important factors influencing different aspects of these processes . - pubmed
Microcystins ( MCs ) represent a group of & gt ; 80 cyclic heptapeptides , mediating cytotoxicity via specific protein phosphatase ( PP ) inhibition at equimolar concentrations ( comparable toxicodynamics ) . - pubmed
Further clinical studies evaluating the ideal moment of fish oil LE infusion to modulate leukocyte functions may contribute to a better understanding of its immune modulatory properties . - pubmed
Flavopiridol protects against inflammation by attenuating leukocyte - endothelial interaction via inhibition of cyclin - dependent kinase 9 . - pubmed
OBJECTIVE : The cyclin - dependent kinase ( CDK ) inhibitor flavopiridol is currently being tested in clinical trials as anticancer drug . - pubmed
Endothelial cells were found to be the major target of flavopiridol , which blocked the expression of endothelial cell adhesion molecules ( intercellular adhesion molecule-1 , vascular cell adhesion molecule-1 , and E - selectin ) , as well as NF - kappaB - dependent transcription . - pubmed
Flavopiridol did not affect inhibitor of kappaB ( IkappaB ) kinase , the degradation and phosphorylation of IkappaBalpha , nuclear translocation of p65 , or nuclear factor - kappaB ( NF - kappaB ) DNA - binding activity . - pubmed
By performing a cellular kinome array and a kinase activity panel , we found LIM domain kinase-1 ( LIMK1 ) , casein kinase 2 , c - Jun N - terminal kinase ( JNK ) , protein kinase C ( PKC ) , CDK4 , CDK6 , CDK8 , and CDK9 to be influenced by flavopiridol . - pubmed
Using specific inhibitors , as well as RNA interference ( RNAi ) , we revealed that only CDK9 is responsible for the action of flavopiridol . - pubmed
CONCLUSIONS : Our study highlights flavopiridol as a promising antiinflammatory compound and inhibition of CDK9 as a novel approach for the treatment of inflammation - associated diseases . - pubmed
Serum estradiol levels increased from 20.9 pg / mL at 0 h to 461 pg / mL at 48 h. Northern analysis was performed for analysis of TIMP-1 , TIMP-2 , and TIMP-3 expression ( N = 4 ) . - pubmed
Continuum source tungsten coil atomic fluorescence spectrometry . - pubmed
A simple continuum source tungsten coil atomic fluorescence spectrometer is constructed and evaluated . - pubmed
A 25 muL sample aliquot is pipetted onto the tungsten coil , the liquid is dried at low current , and then the atomic vapor is produced by applying a current in the range 3.5 - 5.5 A. - pubmed
Orally - administered prostanoid DP receptor antagonists S-5751 and pinagladin significantly suppressed not only bronchial hyperresponsiveness and lung eosinophilia but also neutrophilia and mucus secretion in the lung , while oral prednisolone inhibited only bronchial hyperresponsiveness and eosinophil infiltration . - pubmed
In addition , prostanoid DP receptor antagonists significantly suppressed interleukin ( IL)-1beta , IL-6 and CXCL1 mRNA in contrast to suppression of IL-4 and CCL11 mRNA by prednisolone . - pubmed
Batch and repeated batch cultivation using calcium alginate immobilized cells were studied for alkaline protease production in submerged fermentation . - pubmed
During the long development of hemoglobin ( Hb)-based O ( 2 ) carriers , many side effects of cell - free Hb molecules have arisen , and have implied the physiological importance of the cellular structure of red blood cells ( RBCs ) . - pubmed
However , following rumen biohydrogenations , these FAs could still be metabolised in various tissues / organs involved in the FA metabolism such as the liver and adipose tissues ( ATs ) , thus affecting their subsequent deposition in muscles . - pubmed
Hepatic secretion of ALA as part of the very - low - density lipoprotein particles was lower than that of LA ( -58 % and -23 % for C and LR diets respectively , P = 0.02 ) . - pubmed
They were efficiently taken up by ATs ( & gt;68 % of FA present in the medium ) , with uptake being higher for IM than for SC AT ( +12 % , P = 0.01 ) . - pubmed
Both FAs were mainly esterified ( & gt;97 % of FA present in adipocytes ) into neutral lipids ( & gt;85 % of esterified FA ) . - pubmed
Glucose - aspirin ( GA ) was synthesized by conjugating aspirin ( ASA ) to glucose . - pubmed
The water solubility and biological activity of GA was studied in comparison to aspirin . - pubmed
The human serum protease activity on the ester showed a slower hydrolysis rate , compared to ASA . - pubmed
In conclusion , GA is a highly water soluble derivative of aspirin . - pubmed
Stable Smad7 overexpression significantly inhibited the absorbance in the MTT - dye assay and inhibited the levels of PCNA compared with those in empty vector - transfected cells . - pubmed
Endogenous over - production of the required precursor , lysine , was first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of aspartate kinase III and dihydrodipicolinate synthase . - pubmed
Further disruption of native lysine decarboxylase activity ( by deleting cadA and ldcC ) limited cadaverine by - product formation , enabling lysine production to 2.25 g / L at a glucose yield of 138 mmol / mol ( 18 % of theoretical ) . - pubmed
Co - expression of lysine monooxygenase and 5 - aminovaleramide amidohydrolase ( encoded by davBA ) then resulted in the production of 0.86 g / L AMV in 48 h. Finally , the additional co - expression of glutaric semialdehyde dehydrogenase and 5 - aminovalerate aminotransferase ( encoded by davDT ) led to the production of 0.82 g / L glutarate under the same conditions . - pubmed
A SCOS fabricated with potassium titanyl phosphate crystal as the slab waveguide is shown to have resonance tuning ranges of 6.67 and 22.24 nm , respectively , for TM and TE polarized modes . - pubmed
Their structures were elucidated as ( 25R )- 26 - O - beta - d - glucopyranosyl - furost-5 - ene-12 - one-3beta,22alpha,26 - triol-3 - O - beta - d - glucopyranosyl -( 1 2 )-[ beta - d - xylopyranosyl -( 1 3 )]- beta - d - glucopyranosyl -( 1 4 )- beta - d - galactopyranoside ( 1 , typaspidoside A ) , ( 25S )- 26 - O - beta - d - glucopyranosyl - furost-5 - ene-12 - one-3beta,22alpha,26 - triol-3 - O - beta - d - glucopyranosyl -( 1 2 )-[ beta - d - xylopyranosyl -( 1 3 )]- beta - d - glucopyranosyl -( 1 4 )- beta - d - galactopyranoside ( 2 , 25S - typaspidoside A ) , and timosaponin H1 ( 3 ) , based on the integrative spectroscopic analysis of 1D- and 2D - NMR experiments , ESI - MS data and chemical evidence . - pubmed
To get a vivid description of the microenvironment , we added hydrogen - bond - donor ( HBD ) as well as non - hydrogen - bond - donor ( NBD ) core solvents . - pubmed
For experimental purposes , we used water , ethylene glycol ( EG ) , glycerol ( GY ) as HBD solvents and N , N - dimethyl formamide ( DMF ) as a NBD solvent . - pubmed
With increasing amount of core solvents , irrespective of HBD or NBD , the fluorescence intensity and lifetime of curcumin increase with remarkable red - shift inside the reverse micelle . - pubmed
RESULTS : Immunohistochemistry analysis showed significant increase of GSK-3beta in the cerebral cortex of 7 - month - old APP group ( compare to control group P = 0.003 ) , while the GSK-3beta expression of donepezil or GEPT group were all significantly decreased ( Donepezil vs APP : P = 0.041 ; GI vs APP : P = 0.049 ; Gm vs APP : P = 0.029 ; Gh vs APP : P = 0.036 ) . - pubmed
And the GSK-3beta expression in donepezil and GEPT groups were all decreased . - pubmed
Short - term cuprizone exposure resulted in a selective decrease of alanine , glycine , and proline plasma levels , which was paralleled by an increase of apoptotic cells in the liver and a decrease of alanine aminotransferase in the serum . - pubmed
Our results suggest that cuprizone - induced liver dysfunction results in amino acid starvation and in consequence to the activation of an AAR . - pubmed
Furthermore , the membrane toxicity of all applied saponins was analyzed using extracellular LDH quantification and the general cytotoxicity was analyzed using a colorimetric MTT - assay and DNA quantification . - pubmed
The sampled particles were extracted from the polyurethane foam ( PUF ) impaction substrates using a previously developed and validated protocol , and used in a cellular assay to assess oxidative stress . - pubmed
Attenuation of skeletal muscle and renal injury to the lower limb following ischemia - reperfusion using mPTP inhibitor NIM-811 . - pubmed
Renal peroxynitrite concentration , serum TNF - alpha and IL-6 levels were measured . - pubmed
Serum K+ and necroenzyme levels were significantly lower in the NIM - IR group than in the IR group ( LDH : p<0.001 ; CK : p<0.001 ; K+ : p = 0.017 ) . - pubmed
Serum TNF - alpha and IL-6 levels were significantly lower ( TNF - alpha : p = 0.003 , IL-6 : p = 0.040 ) as well as W / D ratio and peroxynitrite concentration were significantly lower ( p = 0.014 ; p<0.001 ) in the NIM - IR group than in the IR group . - pubmed
The industrialisation of lignocellulose conversion is impeded by expensive cellulase enzymes required for saccharification in bioethanol production . - pubmed
Addition of surfactant , Tween 80 and metal ion Mn (+ 2 ) , significantly enhanced cellulase yield . - pubmed
Here , we describe the implementation of a picosecond time - resolved X - ray photoelectron spectroscopy ( TRXPS ) technique at the Advanced Light Source ( ALS ) and its application to monitor photoinduced electron dynamics at the technologically pertinent interface formed by N3 dye molecules anchored to nanoporous ZnO. - pubmed
In the present study , a microporous silicone rubber membrane bilayer ( SRM - B ) composed of two layers with different pore sizes was prepared . - pubmed
Morphological control revealed a 70 % and 35 % decreases in the amount of tyrosine hydroxylase positive neurons in substancia nigra / pars compacta after 48 and 24 mg / kg of MPTP , respectively , as compared to the saline group . - pubmed
Impact of estrogen and progesterone receptor expression on the clinical and molecular features of papillary thyroid cancer . - pubmed
INTRODUCTION : Maraviroc is a first - in - class antiretroviral ( ARV ) drug acting on a host cell target ( CCR5 ) , which blocks the entry of the HIV virus into the cell . - pubmed
AREAS COVERED : This drug discovery case history focuses on the key studies that led to the discovery and approval of maraviroc , as well as on post - launch clinical reports . - pubmed
The CCR5 - antagonist mariviroc , with its unique mode of action and excellent safety profile , is an important therapeutic option for HIV patients . - pubmed
In this study , we used kinase profiling analysis to demonstrate that 2 '- hydroxycinnamicaldehyde ( 2 '- HCA ) , a compound found in cinnamon , specifically inhibits Pim-1 activity . - pubmed
Cocrystallography studies determined the hydrogen bonding pattern between 2'-HCA and Pim-1 . - pubmed
Notably , 2 '- HCA binding altered the apo kinase structure in a manner that shielded the ligand from solvent , thereby acting as a gatekeeper loop . - pubmed
Overall , our results offered preclinical proof of concept for 2 '- HCA as a potent anticancer principle arising from direct targeting of the Pim-1 kinase . - pubmed
CONCLUSION : Uncomplicated soft - tissue wounds caused by an automatic M70AB2 rifle may be closed primarily with delayed suture without the risk of developing complications if on revision , 24 h after primary surgery , there were no present necrotic tissues , hematoma , and any signs of infection when fibrin glue with antibiotics ( ceftriaxone and clindamycin ) was applied . - pubmed
However , the Cd concentrations reached up to 2.5905 , 3.1628 , and 4.8593 mg / kg in the protein isolates , respectively . - pubmed
Therefore , 10 common acids in food industry were investigated to remove Cd from protein isolate by washing process . - pubmed
Polo - like kinase 1 ( PLK1 ) is a serine / threonine kinase that plays multiple and essential roles during the cell division cycle . - pubmed
SIRT2 activates G6PD to enhance NADPH production and promote leukaemia cell proliferation . - pubmed
Knockdown of G6PD reduces NADPH level in acute myeloid leukaemia ( AML ) cell lines . - pubmed
Deacetylase SIRT2 promotes NADPH production through deacetylating G6PD at lysine 403 ( K403 ) . - pubmed
Influence of filtration velocity on DON variation in BAF for micropolluted surface water treatment . - pubmed
Therefore , to control DON in BAF effluent , it is necessary to study the influence of BAF operation parameters on DON production . - pubmed
Although plasma polymerization is traditionally considered as a substrate - independent process , we present evidence that the propensity of a substrate to form carbide bonds regulates the growth mechanisms of plasma polymer ( PP ) films . - pubmed
Zirconium , titanium , and silicon substrates were used to study the early stages of PP film formation from a mixture of acetylene , nitrogen , and argon precursor gases . - pubmed
On the contrary , the lower propensity of the silicon surface to form carbides leads to a 3D , island - like ( Volmer - Weber ) growth mode that creates a sponge - like interphase near the substrate , resulting in inferior adhesion and poor film stability in SBF . - pubmed
[ 18F - FDG PET / CT findings of primary intestinal lymphoma : analysis of 23 cases ] . - pubmed
CONCLUSION : PIL presents with characteristic imaging features in 18F - FDG PET / CT . - pubmed
18F - FDG PET / CT is a sensitive imaging modality for detecting inter- and extra - intestinal lesions of PIL and displays characteristic imaging features of the disease . - pubmed
Expression of the NtEXGT gene in leaves was induced by cytokinins , auxins , brassinosteroids and gibberellins . - pubmed
NtEXGT gene was also up - regulated by salinity , drought , cold , cadmium and 10 muM abscisic acid treatments and down - regulated in response to 0 C and 100 muM abscisic acid . - pubmed
Pretreatment of leaves with fluridone contributed to smaller increase in the level of NtEXGT transcripts in response to drought stress . - pubmed
These data suggest that NtEXGT gene is ABA - regulated and probably implicated in ABA - dependent signaling in response to stress factors . - pubmed
These results support a novel connection between HIF-1alpha and Pak4 in hypoxic cancer cells , and provide insights into mechanisms whereby tumors respond to and thrive under oxygen - deficient conditions . - pubmed
We show that after a tumor antigen contact the IL-2 activated CD8 + lymphocytes acquire ability to lyse tumor cells bearing this antigen . - pubmed
However , activation of the CD8 + lymphocytes in the absence of antigen causes appearance of a cytotoxic population of CD8 + NKG2D+ lymphocytes , which are able to lyse HLA - negative cancer cells that have lost the classic mechanism of antigen presentation . - pubmed
Increased type 2 diabetes risk in HFE hemochromatosis is associated with one or more factors , including abnormal iron homeostasis and iron overload , decreased insulin secretion , cirrhosis , diabetes in first - degree relatives , increased body mass index , insulin resistance , and metabolic syndrome . - pubmed
In p. C282Y homozygotes , serum ferritin , usually elevated at hemochromatosis diagnosis , largely reflects body iron stores but not diabetes risk . - pubmed
Routine iron phenotyping or HFE genotyping of patients with type 2 diabetes is not recommended . - pubmed
These results suggest that paraquat degradation can be attributed to the presence of extracellular enzymes from white rot fungi . - pubmed
PURPOSE OF REVIEW : It is widely accepted that sugar sweetened beverages ( SSB ) are implicated in weight gain and adverse cardiometabolic heath . - pubmed
Findings from short - term trials of SSB and markers of cardiometabolic risk including lipids , glucose , blood pressure , and inflammatory cytokines provide mechanistic support for associations with T2D and CHD . - pubmed
These include anterior segment photography , specular microscopy , in vivo confocal microscopy , anterior segment optical coherence tomography ( AS - OCT ) , ultrasound biomicroscopy ( UBM ) , laser flare photometry , and less commonly used techniques such as anterior segment fluorescein and indocyanine green angiography , iris autofluorescence , and infrared imaging . - pubmed
Recombinant OsPKS2 catalyzed the condensation of fatty acyl - CoA with malonyl - CoA to generate triketide and tetraketide alpha - pyrones , the main components of pollen exine . - pubmed
However , unlike pksb / lap5 , in which most detected phenolics were substantially decreased , ospks2 accumulated higher levels of phenolics . - pubmed
Furthermore , the study also permitted the identification of ten novel prospective allosteric sites named NP1 to NP10 , involving in most of the cases protein structural elements that control kinase activation including the activation loop , the catalytic loop , the alphaC helix , the L16 loop , and the glycine - rich loop . - pubmed
Dual expression of constitutively active Galphas - protein - coupled receptors differentially establishes the resting activity of the cAMP - gated HCN2 channel in a single compartment . - pubmed
Therefore , some proportion of HCN2 channels opens in response to the basal cAMP pool size produced by constitutively active GPCRs . - pubmed
Thus , we conclude that the dual expression of constitutively active GPCRs establishes the diverse range of the basal cAMP pool size in resting cells through mutual additive or suppressive interactions , even in the absence of external stimulation . - pubmed
Outer retinal reflectivity was measured on volumetric spectral - domain ( SD)-OCT scans acquired 3 months after DME resolution , from the photoreceptor ellipsoid zone ( EZ ) and the retinal pigment epithelium ( RPE ) . - pubmed
Each 1 ng / ml decrease in sMMP-9 after NAC was shown to result in a 0.3 % increase in pCR rate . - pubmed
The methanolic extract ( ME ) was rich in flavonoids , while the powdered leaves ( PL ) contained considerable amounts of macro- and microelements . - pubmed
PL , ME and GUA did not significantly alter the plasma levels of triglycerides , high - density lipoproteins , very low - density lipoproteins , creatinine and aspartate transaminase , and the total protein levels in the kidney and liver tissues . - pubmed
The glyoxalase pathway evolved early in phylogeny to bring about rapid catabolism of methylglyoxal , and an understanding of the role of methylglyoxal and the glyoxalases in many diseases is beginning to emerge . - pubmed
Bonferroni post - hoc testing showed there was statistically significant increases in metabolite ratios between 0700 and 1700 and these include different moieties of N - acetylaspartate , creatine , choline , myo - inositol , lipids , fucose , glutathione , and homocarnosine . - pubmed
The production of reactive oxygen species ( ROS ) as well as the response to oxidative stress and ER stress were investigated . - pubmed
Huntington ' s disease ( HD ) is a monogenic neurodegenerative disorder caused by an expansion of the CAG trinucleotide repeat domain in the huntingtin ( HTT ) gene , leading to an expanded poly - glutamine ( polyQ ) stretch in the HTT protein . - pubmed
The results of alizarin red staining and alkaline phosphatase activity tests showed that VD3 could not induce osteogenic differentiation in HUCMSCs . - pubmed
IgG - conjugated AuNP were treated with NaCN to dissolve the AuNP and restore the fluorescence emission that was quenched in the presence of the metallic colloids , followed by quantification of fluorescein by spectrofluorimetry . - pubmed
SKAP2 functions in reactive oxygen species ( ROS ) production , phagocytosis , and integrin - mediated migration by neutrophils . - pubmed
Foliar anthocyanins are hypothesised to function as photoprotective visible light screens , preventing over - excitation of the photosynthetic system , and decreasing the likelihood of photo - oxidative stress by absorbing green light and reducing the amount of light available to be absorbed by chloroplasts in deeper tissue layers . - pubmed
However , profiles of green light absorption by chlorophyll within anthocyanic leaves were not shifted to reflect reduced absorption of green light by spongy mesophyll chloroplasts . - pubmed
Natural products can inhibit different coronavirus targets such as S protein ( emodin , baicalin ) and viral enzymes replication such as 3CLpro ( Iguesterin ) , PLpro ( Cryptotanshinone ) , helicase ( Silvestrol ) , and RdRp ( Sotetsuflavone ) . - pubmed
Curcuminoids have been shown to exert their anti - GBM effects through regulation of angiogenesis , apoptosis , autophagy , metastasis , invasion as well as potential molecular targets , including receptor tyrosine kinases , Sonic Hedgehog , and NF - kappaB. - pubmed
Pooled risk ratio ( RR ) of having any sperm abnormality and Testosterone , FSH , and LH standardized mean differences among male cannabis users and non - users , and meta - regression analysis according to age , and year of publication . - pubmed
Notably , we identified that 17 - DMAG , which targets the ATP - binding site of heat shock protein 90 ( Hsp90 ) , has a very high ( sub - micromolar range ) selective inhibition of the parasite growth . - pubmed
It could be shown that the 5S RNA synthesized in vitro differed only very slightly in size from the mature species labeled in vivo and contained a few extra nucleotides in some of the molecules . - pubmed
Digestion of the in vitro product with ribonuclease T1 and analysis of the oligonucleotides on DEAE - Sephadex A-25 in the presence of 7 M urea revealed that the molar yield of the internal fragments agreed well with the expected theoretical values . - pubmed
Neither atropine nor pramiverine were able to exert a tone depressing effect as pronounced as that of SSB . - pubmed
A significant correlation between the urinary hEGF levels and creatinine clearance ( r = 0.712 , p less than 0.001 ) and an inverse correlation between urinary hEGF levels and fractional excretion of sodium ( r = -0.406 , p less than 0.05 ) was demonstrated . - pubmed
Pretreating the cells with pertussis toxin to enhance cAMP responses via inhibition of Gi potentiated the effect of bPTH -( 1 - 34 ) and bPTH -( 2 - 34 ) and disclosed weak but detectable agonist action of pbPTH -( 2 - 34 ) . - pubmed
These results indicate that specific domains of the PTH molecule are linked to activation of different second messenger pathways ; while the first two amino acids are indispensable for activating the cAMP system , generation of the [ Ca2+]i signal appears to involve a longer domain , including the amino acid residue in position 3 . - pubmed
[ Effects of EGF and retinoic acid on differentiation of squamous cell carcinoma cells ] . - pubmed
Modification of gizzard myosin and reconstituted actomyosin with 7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole at low and high ionic strength . - pubmed
Treatment of reconstituted gizzard actomyosin at 0.15 M or 0.6 M KCl with the fluorescent adenine analog 7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole , NBD - Cl , resulted in a significant decrease in the labeling of the myosin from actomyosin compared to that of myosin alone . - pubmed
The reagent was able to detect changes in the conformation of myosin as the distribution of the label in the heavy and light chains of myosin and actin was different at 0.15 M and 0.6 M KCl . - pubmed
Jejunal mucosa is responsible for the absorption of triglycerides and the production of lipoproteins [ chylomicrons , very - low - density lipoprotein ( VLDL ) , high - density lipoprotein ( HDL ) ] and apolipoproteins ( B-48 , A - I , A - II , A - IV , C - II ) . - pubmed
Statistically significant differences were found in the following parameters ( celiac versus control ): plasma levels of triglycerides ( 70 versus 119 mg / dl ) , cholesterol content in LDL ( 107 versus 67.7 mg / dl ) , protein content in VLDL ( 6 versus 10 mg / dl ) , and level of apoprotein A - I ( 112 versus 140 mg / dl ) . - pubmed
No significant differences were found between the two groups in the serum levels of total cholesterol , the cholesterol content in VLDL and HDL , the protein content in LDL and HDL , and the level of apoprotein B. - pubmed
The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters . - pubmed
On all five tobraviral RNA2 molecules sequenced to date , these sequences were found upstream of the coat protein ORF in association with a strongly - conserved potential secondary structural element . - pubmed
Upon stimulation with the antigen ovalbumin ( OA ) of cells passively sensitized with antiovalbumin ( anti - OA ) antibody , both cell types released histamine . - pubmed
Both lung mast cells and blood basophils released histamine in response to the secretagogues calcium ionophore A23187 and 12 - O - tetradecanoylphorbol-13 - acetate ( TPA ) ; TPA - induced HR from mast cells was independent of added calcium . - pubmed
In basophils , TPA - induced HR was only partially independent of added calcium . - pubmed
Phosphatidylserine had no effect on HR provoked by antigen , Con - A , or compound 48 / 80 . - pubmed
For the latter strains , the data suggested an important role of the nucleotide at , and perhaps around , position 472 in determining a response to the addition of ICF . - pubmed
Amiloride selectively inhibits the urokinase - type plasminogen activator . - pubmed
The diuretic drug amiloride , an inhibitor of Na + uptake , competitively inhibits the catalytic activity of the urokinase - type plasminogen activator ( u - PA ) , with a Ki of 7 X 10(-6 ) M. Generation of plasmin , cleavage of peptide substrates , and interaction of u - PA with a specific macromolecular proteinase inhibitor are all prevented in the presence of the drug . - pubmed
In contrast , amiloride does not affect the activity of either tissue - type plasminogen activator , plasmin , plasma kallikrein or thrombin . - pubmed
The inhibition of u - PA by amiloride may be related to the previously reported inhibition of u - PA - type enzymes by Na + . - pubmed
Amiloride or related compounds could prove useful in selectively controlling u - PA - catalyzed extracellular proteolysis . - pubmed
The microsomal enzyme activity reduced free and bound retinaldehyde to approximately the same extent , although the aldehyde function of retinaldehyde bound to CRBP ( II ) was less accessible to chemical reducing agents than that of free retinaldehyde . - pubmed
Retinol bound to CRBP ( II ) could not be oxidized by the microsomal activity in the presence of NAD + , while free retinol or retinol bound to bovine serum albumin was oxidized to retinaldehyde . - pubmed
A rise of serum potassium ( mean + /- SD mmol / L ) from 4.1 + /- 0.3 to 4.4 + /- 0.4 ( p less than 0.05 ) in the placebo group and from 4.0 + /- 0.4 to 4.5 + /- 0.5 ( p less than 0.05 ) in the timolol group was in multivariate analysis associated with infarct size , estimated as cumulative creatine kinase release , in the placebo group , and with the mean individual plasma adrenaline concentrations in the timolol group . - pubmed
All the preparations were shown to contain a ribonuclease which , in the presence of ethylenediaminetetraacetic acid , led to ribosome breakdown as measured by the increase in acid - soluble nucleotides . - pubmed
In conjunction with a changed capacity for RNA synthesis it was observed that abscisic acid - treated segments had a lowered soluble DNA - dependent RNA polymerase level in comparison to gibberellic acid - treated or illuminated segments . - pubmed
Interaction of helper T cells and Lyb5 + B cells responding to phosphorylcholine is MHC - restricted . - pubmed
[ Inhibition of IgE antibody formation by tubercle bacilli and muramyl peptide ] . - pubmed
Seven major tryptic peptides of the enzyme were found to be cross - linked to tRNAPhe whereas six major T1 - oligonucleotides were covalently linked to the protein , among these , the three cross - linked oligonucleotides previously described by Shoemaker and Schimmel ( J. Biol . - pubmed
The occurrence of covalent cross - linking upon irradiation in the tryptophan absorption band ( 302 nm ) strongly suggests the participation of this residue in the stabilization of the tRNA enzyme complex . - pubmed
Response of insulin receptors to oral glucose in normal subjects . - pubmed
Effect of celiprolol and metoprolol on lipids , fibrinogen and airways function in hyperlipidaemic hypertensives : a randomised double - blind long - term parallel group trial . - pubmed
Significant decreases in systolic and diastolic blood pressure and heart rate were observed compared with baseline ( DBP : celiprolol -13.3 mm Hg , P & lt ; 0.0001 ; metoprolol -14.3 mm Hg , P & lt ; 0.0001 ; SBP : celiprolol -18.2 mm Hg , P & lt ; 0.0001 ; metoprolol -20.5 mm Hg , P & lt ; 0.0001 ; heart rate celiprolol -4 beats / min , P & lt ; 0.003 ; metoprolol -12 beats / min , P & lt ; 0.0001 ) . - pubmed
When compared with baseline values celiprolol significantly reduced serum low density lipoprotein cholesterol ( LDL - C ) ( -5.8 % , P = 0.0401 ) and produced a slight increase in high density lipoprotein cholesterol ( HDL - C ) which approached statistical significance ( 4.1 % , P = 0.0659 ) . - pubmed
Metoprolol significantly increased serum triglycerides ( 32 % , P = 0.0001 ) and the total / HDL - C ratio ( 7.4 % , P = 0.0192 ) . - pubmed
Compared with metoprolol , celiprolol significantly reduced LDL - C ( -7.3 % , P = 0.0062 ) , total cholesterol ( -4.5 % , P = 0.0085 ) , apoliproprotein B ( -10.1 % , P = 0.0001 ) , the apolipoprotein B / A1 ratio ( -10.9 % , P = 0.0001 ) , the total cholesterol / HDL - C ratio ( -10.8 % , P = 0.0001 ) and triglycerides ( -24.8 % , P = 0.0001 ) , and significantly increased HDL - C ( 6.0 % , P = 0.0043). - pubmed
Serotonin antagonism did not noticeably affect the LH pulse attributes ( frequencies , interpulse intervals , amplitudes ) . - pubmed
Gonadotrophin and PRL release in response to GnRH administration was unaltered by ondansetron treatment . - pubmed
The identical effects of glycine with that of glutamate in certain concentrations is consistent with previous reports that glycine and N - methyl - D - aspartate ( NMDA ) act as coagonists of a common excitatory amino acid receptor . - pubmed
Effect of chronic central treatment with the acetylcholine analog methylcarbamylcholine on cortical nicotinic receptors : correlation between receptor changes and behavioral function . - pubmed
Chronic administration of nicotine results in increased numbers of specific nicotinic acetylcholine receptors ( nAChRs ) within the central nervous system . - pubmed
Chronic administration of MCC reversibly increased the apparent Bmax and Kd of cortical nAChRs in a dose- and time - dependent manner . - pubmed
Like chronic MCC treatment , chronic DHBE increased the number of [ 3H ] cytisine binding sites but without concomitant development of behavioral tolerance. - pubmed
In 0.1 M KCl between pH 6.3 and pH 7.3 , filtered samples were turbid , suggesting the presence of high molecular weight aggregates ( mesophase ) , which could be removed by ultrafiltration . - pubmed
METHODS : Blood pressure , demographic factors , plasma renin activity ( PRA ) , urinary electrolyte excretion and bioassayable PHF - like activity were determined in the fasted state on basal and altered dietary salt intakes . - pubmed
This latter finding provides a strong warning that p53 immunochemistry with available reagents can not be used to determine which tumors have mutations of p53 . - pubmed
Nitric oxide ( NO ) , a free radical gas that acts as a neural messenger , is produced from L - arginine by the enzyme nitric oxide synthase ( NOS ) . - pubmed
[ 3H ] L - NOARG binding was enriched in discrete neuronal populations and the distribution observed was identical to that previously described for NOS using immunohistochemistry , in situ hybridization and NADPH - diaphorase staining . - pubmed
[ 3H ] L - NOARG autoradiography should be a useful method for the further characterization and quantification of NOS in brain and other tissues . - pubmed
Bradykinin analogs were synthesized by the solid phase method , using Boc strategy on PAM or Merrifield resins . - pubmed
The same analog completely inhibits bradykinin - evoked cytokine expression by mononuclear cells . - pubmed
Pulse oximetry has become the leading technique for monitoring hemoglobin oxygen saturation whenever a risk of hypoxia exists . - pubmed
Since it uses 2 wavelengths , it can only measure oxyhemoglobin and reduced hemoglobin . - pubmed
Ethanol feeding impairs innate immunity and alters the expression of Th1- and Th2 - phenotype cytokines in murine Klebsiella pneumonia . - pubmed
The prolonged and excessive consumption of alcohol has been shown to predispose the host to a variety of infectious complications , which may be due , in part , to the inability to produce important activating cytokines . - pubmed
In summary , our studies indicate that ethanol ingestion results in a profound suppression of lung bacterial clearance and decreased survival in Klebsiella pneumonia , which occurs in association with a shift in the balance of lung cytokine mRNA and protein expression favoring Th2- rather than Th1 - phenotype cytokines . - pubmed
Understanding transcription initiation of the endothelial nitric - oxide synthase ( eNOS ) gene appears pivotal to gaining a comprehensive view of NO biology in the blood vessel wall . - pubmed
EPR of a VO2 + -doped Mg ( ND4 ) 2 ( SO4 ) 2 - pubmed
Of the 10 models generated by the program Catalyst / Hypo , hypotheses including hydrogen - bond acceptor ( HBA ) , hydrophobic ( Hp ) , and hydrophobic aliphatic ( HpAl ) features were considered important and predictive in evaluating OA agonists . - pubmed
An HBA and four hydrophobic features are the minimum components of an effective OA agonist - binding hypothesis , which resembles the results of binding activity to locust OAR3 . - pubmed
Active agonists mapped well onto all of the features of the hypothesis , such as HBA , Hp , and HpAl features . - pubmed
Enoxaparin was administered 2 h post - TBI at 0.5 mg / kg i.v . - pubmed
The neurological deficit evaluated with a 9 - point scale was also improved with enoxaparin at 0.5 + 4 x 1 mg / kg 1 week post - TBI ( p & lt ; 0.05 ) . - pubmed
The cognitive impairment evaluated with a Lashley maze task was improved with enoxaparin ( 0.5 + 4 x 1 mg / kg ) from 48 h ( p & lt ; 0.05 ) to 2 weeks post - TBI ( p & lt ; 0.01 ) . - pubmed
Aza analogues of thalidomide : synthesis and evaluation as inhibitors of tumor necrosis factor - alpha production in vitro . - pubmed
These 5 - phthalimidopyrimidines as well as phthalimido-2,4 - difluorobenzenes were designed as analogues of thalidomide , a well known inhibitor of TNF - alpha production . - pubmed
HT , DM , low levels of high - density lipoprotein cholesterol ( HDL - C ) and hypertriglyceridemia remained as significant coronary artery disease ( CAD ) risk factors even after controlling for age , sex , hospital , smoking , alcohol use , body mass index and leisure time physical activity . - pubmed
On multivariate analysis , preischemic FAC(AUC ) was mainly affected by sex , peak FAC(AUC ) was affected by sex and systolic BP , percent changes were affected by plasma high - density and low - density lipoprotein cholesterol , peak time was affected by age and body mass index , and descent time was affected by plasma triglyceride levels . - pubmed
The enzyme L - phenylalanine ammonia - lyase ( PAL ) catalyzes the non - oxidative deamination of L - phenylalanine to form trans - cinnamic acid and ammonia . - pubmed
X - ray diffraction ( XRD ) , Brunauer Emmett Teller method ( BET ) , electron probe X - ray micro analysis ( EPMA ) and temperature programmed reduction ( TPR ) by H2 were used to characterize a series of supported copper catalysts . - pubmed
Inflammation induces an up - regulation of neuropeptide tyrosine ( NPY ) and its receptors in the dorsal horn , suggesting an important role in nociceptive transmission . - pubmed
The ipsilateral effect of NPY was completely inhibited with the potent Y1 antagonist , BIBO 3304 ( 3 microg ) , but not the Y2 antagonist , BIIE 0246 . - pubmed
When administered alone , BIBO 3304 ( but not BIIE 0246 ) slightly decreased thermal PWL on the side ipsilateral ( 25 % change ) , but not contralateral , to CFA injection ; this suggests that inflammation strengthens inhibitory NPY tone . - pubmed
Sleep attacks in Parkinson ' s disease induced by Entacapone , a COMT - inhibitor . - pubmed
Entacapone , a catechol - O - methyl transferase inhibitor , alters the pharmacokinetics of levodopa , leading to increase of levodopa concentration in plasma and brain . - pubmed
They were submitted to clinical history , laboratory studies ( blood count cell , serum IgE levels , eosinophils of nasal mucosa ) , roentgenograms ( paranasal sinus and esophagus - gastroduodenal series ) and allergy skin tests with 32 allergens . - pubmed
Testosterone significantly increased claudin-11 expression in cultured Sertoli cells . - pubmed
Angiotensin II blocks nicotine - mediated neuroprotection against beta - amyloid ( 1 - 42 ) via activation of the tyrosine phosphatase SHP-1 . - pubmed
We also showed that preincubation with angiotensin II ( Ang II ) , functioning via the angiotensin II type 2 ( AT2 ) receptor , blocked both the nicotine - induced activation of JAK2 and its neuroprotection against Abeta ( 1 - 42 ) . - pubmed
Therefore , the potential biological significance of AT2 receptor - induced effects on both the nicotine - induced activation of JAK2 and its neuroprotection against Abeta ( 1 - 42 ) led us to investigate whether SHP-1 activation could be involved in this process . - pubmed
We found that Ang II induced the activation of SHP-1 and that an antisense against SHP-1 not only augmented the nicotine - induced tyrosine phosphorylation of JAK2 but also blocked the Ang II neutralization of the nicotine - induced neuroprotection . - pubmed
The adsorption of 3 barbiturates -- phenobarbital , mephobarbital , and primidone -- from simulated intestinal fluid ( SIF ) , without pancreatin , by activated carbon was studied using the rotating bottle method . - pubmed
rAd MEK1EE infection induced morphological changes and DNA synthesis which were inhibited by the MEK1,2 inhibitor PD184352 . - pubmed
This secretion is inhibited by PD184352 , but not by ErbB inhibitors . - pubmed
As UV - B had no influence on CYP1A content in larval pike , retene ( 9 - 82 microg / l ) induced this protein substantially with and without UV - B. - pubmed
Neither UV nor retene changed SOD activity significantly . - pubmed
Changing the extracellular matrix protein to laminin - rich Matrigel , the monolayers re - formed islet - like clusters that secreted insulin in a glucose - responsive fashion . - pubmed
Myotoxic bupivacaine was injected into SOL and EDL and the muscles were either denervated or neuromuscularly blocked by tetrodotoxin ( TTX ) on the sciatic nerve . - pubmed
Three to 10 or 30 days later , denervated SOL or EDL , or innervated but neuromuscularly blocked EDL received a slow 20 Hz stimulus pattern through electrodes implanted on the muscles or along the fibular nerve to EDL below the TTX block . - pubmed
Stimulated denervated and stimulated innervated ( TTX blocked ) EDL expressed the same amounts of type 1 , 2A , 2X and 2B MyHC proteins . - pubmed
ADC and NADC TUNEL positive dying cells in resin sections observed by light microscopy were examined again by transmission electron microscopy using a re - embedding procedure . - pubmed
High - affinity capture of proteins by diamond nanoparticles for mass spectrometric analysis . - pubmed
Sodium nitroprusside led to an increase in CBF and CBV ( +30 % and +37 % , respectively , P<0.05 ) , whereas no change in CMRO2 was noted . - pubmed
Linear regression analysis of CBF values as a function of MABP confirmed that CBF increases when MABP is reduced by SNP . - pubmed
Carbonyl content in RPE cells increased with increasing concentrations of oxidants or increasing duration of exposure with high reproducibility , validating ELISA for carbonyl content as a valuable quantitative measure of oxidative damage . - pubmed
Compared to other cell types , RPE cells were able to survive exposure to H2O2 quite well and exposure to paraquat extremely well . - pubmed
This study suggests that multiple types of oxidant stress should be used to probe the vulnerabilities of the retina and RPE in vivo , and that ELISA for carbonyl content provides a valuable tool for quantitative assessment of oxidative damage for such studies . - pubmed
Despite limited data , polymyxin B ( PB ) is increasingly used clinically as the last therapeutic option for multidrug - resistant ( MDR ) gram - negative bacterial infections . - pubmed
Mixture and concentration - response experiments demonstrated a suppression of phagocytosis by non - coplanar PCBs suggesting a previously unrecognized aryl hydrocarbon receptor ( AhR)-independent pathway . - pubmed
Our results are cause for concern as they suggest an AhR - independent pathway through which non - coplanar PCBs modulate phagocytosis , the immune system 's first line of defense , possibly increasing the risk to developing infectious disease . - pubmed
Treatment with 0.22 mg of MGA / head / d for 14 d had a detrimental effect on fertility when insemination was carried out during the first estrus post treatment . - pubmed
A reciprocal substitution of amino - acid residues with a hydroxyl group at these positions , T141S or S150 T , produced a broadened cation selectivity of the antiporter for K+ , in addition to Na + and Li+ . - pubmed
Site - directed mutagenesis of Ser150 showed that while the replacement of Ser150 with a small hydrophobic ( valine ) or negatively charged ( aspartate ) amino acid did not produce a significant change in ZrSod2 - 22p substrate specificity , the introduction of a positive charge at this position stopped the activity of the antiporter . - pubmed
Mean seasonally adjusted 25 ( OH ) D concentration was 71.1 nmol / L. Serum 25 ( OH ) D also exhibited the expected inverse curvilinear relationship with serum parathyroid hormone ( PTH ) , with the inflection point of the curve located at approximately 80 nmol / L. Supplements containing vitamin D were regularly taken by 59 % of the cohort ( median dose : 200 IU / d ) . - pubmed
Natural peptide agonists of corticotrophin - releasing factor ( CRF ) receptors bind to the receptor by a two - site mechanism as follows : the carboxyl end of the ligand binds the N - terminal extracellular domain ( ECD ) of the receptor and the amino portion of the ligand binds the extracellular face of the seven transmembrane region . - pubmed
Two hydrophobic residues ( Met(38 ) and Ile(41 ) ) as well as two amide groups ( Asn(34 ) and the C - terminal amide ) on one face of the helix defined the binding epitope of the antagonist . - pubmed
Serum zinc levels of diseased calves were lower than healthy ones and this difference were found to be important statistically ( p<0.001 ) , whereas there is no statistical difference on the levels of lymphocyte , monocyte , granulocyte , erythrocyte , hemoglobin , hematocrit , and mean corpuscular volume between groups . - pubmed
Transcription factors , including PPAR coactivator ( PGC )- 1beta , PGC-1alpha , estrogen - related receptor alpha , and PPARalpha , were suppressed in both models and induced by RSG . - pubmed
The effects of RSG on adipose mitochondrial genes were confirmed by quantitative RT - PCR and further supported by mitochondrial staining , mitochondrial DNA quantification , and citrate synthase activity . - pubmed
Published data on the concentration dependence of osmotic pressure of solutions of bovine serum albumin in 0.15 M NaCl at concentrations up to greater than 400 g / L are shown to be described to within experimental uncertainty by a simple one - parameter model in which protein molecules are represented by effective hard spherical particles . - pubmed
We show that emerin interacts with tubulin , and that nocadozole - treated wild - type cells phenocopy the detached centrosome characteristic of emerin - null / deficient cells . - pubmed
Phorbol 12 - myristate 13 - acetate - activated protein kinase C increased migratory activity of subconjunctival fibroblasts via stress - activated protein kinase pathways . - pubmed
The fibroblasts were treated with phorbol 12 - myristate 13 - acetate ( PMA ) to activate PKC . - pubmed
PMA treatment in the subconjunctival fibroblasts elicited phosphorylation of SAPKs , including c - jun N - terminal kinase ( JNK ) and p38 mitogen - activated protein ( MAPK ) . - pubmed
Furthermore , specific inhibitors of JNK and p38MAPK blocked PMA - induced migration of the subconjunctival fibroblasts . - pubmed
The phosphorylation of c - jun and heat shock protein 27 , downstream effectors of JNK and p38MAPK , respectively , was upregulated by PMA treatment . - pubmed
CONCLUSIONS : PMA - activated PKC increased migratory activity , and SAPK pathways were critical for migration of subconjunctival fibroblasts . - pubmed
We assayed fasting total , high - density lipoprotein ( HDL ) , and low - density lipoprotein ( LDL ) cholesterol ; LDL / HDL cholesterol ratio ; triacylglycerols ( TG ) ; and C - reactive protein ( CRP ) . - pubmed
Dietary GI was significantly associated with HDL and LDL cholesterol , LDL / HDL cholesterol ratio , TG , and CRP ( comparing top to bottom quintile difference in HDL cholesterol = -2.6 mg / dL , LDL cholesterol = 2.2 mg / dL , LDL / HDL cholesterol ratio = 0.16 , TG = 12 mg / dL , and CRP = 0.21 mg / L ) . - pubmed
Dietary GL was associated with HDL cholesterol , LDL / HDL cholesterol ratio , and TG ( comparing top to bottom quintile HDL cholesterol = -4.9 mg / dL , LDL / HDL cholesterol ratio = 0.24 , and TG = 13 mg / dL ) . - pubmed
Upon stimulation by growth factors and chemokine ligands , integrin - linked kinase mediates the phosphorylation of Akt Ser473 , and glycogen synthase kinase-3 . - pubmed
A ternary complex of hydroxycinnamoyl - CoA hydratase - lyase ( HCHL ) with acetyl - CoA and vanillin gives insights into substrate specificity and mechanism . - pubmed
HCHL ( hydroxycinnamoyl - CoA hydratase - lyase ) catalyses the biotransformation of feruloyl - CoA to acetyl - CoA and the important flavour - fragrance compound vanillin ( 4 - hydroxy-3 - methoxybenzaldehyde ) and is exploited in whole - cell systems for the bioconversion of ferulic acid into natural equivalent vanillin . - pubmed
The reaction catalysed by HCHL has been thought to proceed by a two - step process involving first the hydration of the double bond of feruloyl - CoA and then the cleavage of the resultant beta - hydroxy thioester by retro - aldol reaction to yield the products . - pubmed
Kinetic analysis of active - site residues identified using the crystal structure of HCHL revealed that while Glu-143 was essential for activity , Ser-123 played no major role in catalysis . - pubmed
Structures of WT ( wild - type ) HCHL and of the S123A mutant , each of which had been co - crystallized with feruloyl - CoA , reveal a subtle helix movement upon ligand binding , the consequence of which is to bring the phenolic hydroxyl of Tyr-239 into close proximity to Tyr-75 from a neighbouring subunit in order to bind the phenolic hydroxyl of the product vanillin , for which electron density was observed . - pubmed
The active - site residues of ligand - bound HCHL display a remarkable three - dimensional overlap with those of a structurally unrelated enzyme , vanillyl alcohol oxidase , that also recognizes p - hydroxylated aromatic substrates related to vanillin . - pubmed
The data both explain the observed substrate specificity of HCHL for p - hydroxylated cinnamate derivatives and illustrate a remarkable convergence of the molecular determinants of ligand recognition between the two otherwise unrelated enzymes . - pubmed
Fasting blood glucose , HDL , LDL and TSH levels , age , glucose intolerance low serum folic acid and significantly increased polyneuropathy risk . - pubmed
Age , weight , body mass index , high fasting , 30 , 60- , 90- , 120 - min serum glucose , insulin and HgA1c levels were risk factors for glucose intolerance . - pubmed
Potassium channel interacting proteins ( KChIPs ) are members of a family of calcium binding proteins that interact with Kv4 potassium ( K(+ ) ) channel primary subunits and also act as transcription factors . - pubmed
To address this question , we studied the association of AnxA6 with model phospholipid membranes by combining the techniques of quartz crystal microbalance with dissipation monitoring ( QCM - D ) , ( cryo- ) transmission electron microscopy ( TEM ) and atomic force microscopy ( AFM ) . - pubmed
We show that AnxA6 presents two modes of association with lipid membranes depending on Ca ( 2 +)-concentration . - pubmed
At low Ca ( 2 +)-concentration ( approximately 60 - 150microM ) , AnxA6 binds to membranes via its two coplanar annexin modules and is not able to associate two separate membranes . - pubmed
At high Ca ( 2 +)-concentration ( approximately 2mM ) , AnxA6 molecules are able to bind two adjacent phospholipid membranes and present a conformation similar to the AnxA6 3D crystallographic structure . - pubmed
Primary cutaneous CD30 + anaplastic large - cell lymphoma with generalized skin involvement and involvement of one peripheral lymph node , successfully treated with low - dose oral etoposide . - pubmed
Lactate and attentional measurements were performed before ( Block 1 , B1 ) and after the fatiguing exercise ( B2 ) and at the end of a recovery period ( B3 ) . - pubmed
In this study , archival formalin - fixed , paraffin - embedded breast cancer specimens at various developmental stages were prepared as tissue microarrays ( TMAs ) and examined for the expressions of piwil2 , estrogen receptor ( ER ) , progesterone receptor ( PR ) and a cell proliferation marker Ki67 by immunohistochemical ( IHC ) staining and human epidermal growth factor receptor 2 ( HER2 ) by fluorescence in situ hybridization ( FISH ) . - pubmed
Three opioid receptors ( mu , micro ; delta , delta ; and kappa , kappa ) that mediate opioid effects have been identified by molecular cloning : delta ( enkephalin - preferring ) , kappa ( dynorphin - preferring ) , and micro ( morphine and ss - endorphin - preferring ) ( 1 ) . - pubmed
Carfentanil ( CFN ) is a highly potent and selective micro opioid receptor agonist with subnanomolar affinity ( Kd , 0.08 nM ) . - pubmed
[ ( 11 ) C ] CFN is being developed as a positron emission tomography ( PET ) agent for the non - invasive studies of micro opioid receptors in the brain . - pubmed
On the other hand , N1 '- methylnaltrindole ( MeNTI ) is a potent and selective delta opioid receptor antagonist ( Ki , 0.02 nM ) . - pubmed
[ ( 11 ) C ] MeNTI has been prepared for studying the role of delta opioid receptor in health and disease by PET imaging . - pubmed
[ The influence of live ammunition manoeuvres on the levels of serum hormones ( cortisol , testosterone , gastrin , and insulin ) ] . - pubmed
HDACi also decreased the expression of SGLT1 , an active glucose transporter found to be stabilized by EGFR , and suppressed the glucose uptake of cancer cells . - pubmed
This article describes the adaptation of a simple colorimetric assay for inorganic pyrophosphate to the enzyme 3 - deoxy - d - manno - octulosonate cytidylyltransferase ( CMP - KDO synthetase , KdsB , EC 2.7.7.38 ) , a key enzyme in the biosynthesis of lipopolysaccharide ( LPS ) in Gram - negative organisms . - pubmed
This assay is particularly useful because it can be combined with the malachite green ( MG ) assay for inorganic phosphate to form an assay system capable of determining inorganic phosphate and inorganic pyrophosphate in the same solution ( the MG / EK ( eikonogen reagent ) assay ) . - pubmed
We tested this potential using two enzymes , KdsB and KdsC , involved in the biosynthesis and use of the key bacterial 8 - carbon sugar , KDO . - pubmed
They hydrolysed aesculin , starch and urea , but not casein or DNA . - pubmed
The dorsal medulla was exposed , and phenylephrine ( 2 mM , 10 nl ) and then the alpha ( 1 )- adrenoceptor antagonist prazosin ( 0.2 mM , 3 x 40 nl ) were microinjected into the XII nucleus while XII nerve activity ( XIIa ) was recorded . - pubmed
poly ( SMA - co - PEGMEMA - co - EDMA ) with 15 % ( w / w ) PEGMEMA ) demonstrated nearly 35 % reduction in retention of polycyclic aromatic compounds and greater than 40 % increase in retention of phenols compared to a hydrophobic C18 monolithic column . - pubmed
Again , incorporation of 15 % ( w / w ) PEGMEMA in the monolith increased the extraction efficiency of phenols in water from approximately 20 % to 67 - 92 % compared to a hydrophobic C18 monolithic material . - pubmed
The OTC loading rates at between 22.22 and 133.33 g OTC m(-3 ) d(-1 ) improved the hydrolysis of molasses - COD ( k ) , the maximum specific utilization of molasses - COD ( k(mh ) ) and the maximum specific utilization rate of TVFA ( k(TVFA ) ) . - pubmed
Therefore , we examined whether TFDG and EGCG affect MMP activity and osteoclast formation and differentiation in vitro . - pubmed
Numbers of multinucleated osteoclasts and actin rings decreased in polyphenol - treated cultures relative to control cultures . - pubmed
MMP-9 mRNA levels declined significantly in TFDG - treated osteoclasts in comparison to control osteoclasts . - pubmed
TFDG and EGCG inhibited the formation and differentiation of osteoclasts via inhibition of MMPs . - pubmed
TFDG may suppress actin ring formation more effectively than EGCG . - pubmed
In silico analyses and genetic complementation tests showed that entA locates next to an entB ortholog , which codes for a putative protein that contains the isochorismatase lyase domain , which is needed for DHBA biosynthesis from isochorismic acid , but lacks the aryl carrier protein domain , which is needed for tethering activated DHBA and completion of siderophore biosynthesis . - pubmed
Spermatocytes lacking zw10 or rint1 function assemble regular central spindles and acto - myosin rings , but furrow ingression halts prematurely due to defective plasma membrane addition . - pubmed
In order to conduct the mentioned evaluation the restriction fragment length polymorphisms ( ApaI , BsmI and TaqI ) in the Vitamin D receptor gene were researched in three groups of patients : patients suffering only from psoriasis vulgaris , patients suffering only from diabetes mellitus , and patients suffering at the same time from both diseases . - pubmed
The ATP2C1 gene encodes for the secretory pathway calcium ( Ca2 +)-ATPase pump ( SPCA1 ) , which localizes along the secretory pathway , mainly in the trans - Golgi . - pubmed
If dysregulated , the composition of the SPCA1 isoform pool could diverge from the physiological status , affecting cytosolic Ca2 + -signaling , and in turn perturbing cell division , leading to cell death or to neoplastic transformation . - pubmed
The reaction of ( NBu4 )[ ReOCl4 ] with H2L1 and two equivalents of ammonium thiocyanate , however , gives in a one - pot reaction [ ReO ( NCS ) 2 ( HL1 ) ] ( 8) , in which the pro - ligand H2L1 is only singly deprotonated . - pubmed
These results suggest that highly malignant differentiated thyroid cancer can be detected by monitoring HER2 status and telomere shortening , and that trastuzumab therapy may be effective for refractory thyroid cancer . - pubmed
H2 S had little effect on eNOS protein expression in ECs . - pubmed
L - cysteine , a precursor of H2 S , stimulated NO production whereas blockage of the activity of H2 S - generating enzyme , cystathionine gamma - lyase ( CSE ) , inhibited this action . - pubmed
LY294002 ( Akt / PI3 - K inhibitor ) or SB203580 ( p38 MAPK inhibitor ) abolished the effects of H2 S on eNOS phosphorylation , NO production , cell proliferation and tube formation . - pubmed
Blockade of NO production by eNOS - specific siRNA or nitro - L - arginine methyl ester ( L - NAME ) reversed , but eNOS overexpression potentiated , the proliferative effect of H2 S on ECs . - pubmed
Our results suggest that H2 S stimulates the phosphorylation of eNOS through a p38 MAPK and Akt - dependent pathway , thus increasing NO production in ECs and vascular tissues and contributing to H2 S - induced angiogenesis . - pubmed
Here , we demonstrate that Pds5 proteins are essential for cohesion establishment by allowing Smc3 acetylation by the cohesin acetyl transferases ( CoATs ) Esco1/2 and binding of Sororin . - pubmed
NaHS pretreatment restored both the DDAH activity and intracellular l - arginine levels . - pubmed
Extra- and intra - cellular partitions of ADMA seem to depend on transport proteins as well as the DDAH activity . - pubmed
METHODS : The effects of PGD2 , IL-25 , and IL-33 on the cell migration , cytokine production , gene regulation , and receptor expression of ILC2s were measured with chemotaxis , ELISA , Luminex , flow cytometry , quantitative RT - PCR , and QuantiGene assays . - pubmed
RESULTS : PGD2 binding to CRTH2 induced ILC2 migration and production of type 2 cytokines and many other cytokines . - pubmed
CONCLUSIONS : PGD2 is an important and potent activator of ILC2s through CRTH2 mediating strong proallergic inflammatory responses . - pubmed
Thus , the photocatalytic degradation of 4 - chlorophenol ( 4 - CP ) , a potent endocrine disrupting chemical in aqueous medium was investigated by both pure and Mg - doped ZnO NPs under UV - light irradiation in the present study . - pubmed
The influence of the Mg content on the structure , morphology , PL character and photocatalytic activity of ZnO NPs were investigated systematically . - pubmed
The search terms were ' gout ' , ' gouty arthritis ' , ' hyperuricemia ' , ' uric acid ' , ' xanthine oxidase ( XO ) inhibitor ' , ' uricosuric ' , ' urate transporter 1(URAT1 ) ' and ' glucose transporter 9 ( GLUT9 ) ' . - pubmed
In in vitro studies , flavonoids , alkaloids , essential oils , phenolic compounds , tannins , iridoid glucosides , and coumarins show the potential of anti - gout effects by their XO inhibitory action , while lignans , triterpenoids and xanthophyll are acting through their anti - inflammatory effects . - pubmed
Adrenomedullin ( AM ) belongs to a calcitonin gene - related peptide ( CGRP ) family and has been demonstrated to recruit CGRP following chronic use of morphine and neuronal nitric oxide synthase ( nNOS ) in inflammation . - pubmed
The present study investigated the possibility that AM initiates the changes of other molecules contributing to the development of morphine tolerance in its chronic use . - pubmed
co - administration of the AM receptor antagonist AM22 - 52 ( 35.8 mug ) inhibited tolerance to morphine - induced analgesia while a daily injection of the AM receptor agonist AM1 - 50 ( 8 mug , i.t . - pubmed
Persistent exposure of cultured dorsal root ganglion ( DRG ) explants to morphine ( 3.3 muM ) for 4 days resulted in an increase in AM and CGRP mRNA levels . - pubmed
Particularly , the co - administration of AM22 - 52 ( 35.8 mug ) inhibited the morphine - induced alterations in nNOS and BAM22 . - pubmed
These results indicated that the increase in nNOS and CGRP expressions and the decrease in BAM22 were attributed to the increased AM receptor signaling induced by chronic morphine . - pubmed
The present study supports the hypothesis that the enhancement of AM bioactivity triggered upregulation of pronociceptive mediators and downregulation of pain - inhibiting molecule in a cascade contributing to the development of morphine tolerance . - pubmed
Concentrations of prostaglandin E2 ( PGE2 ) , transforming growth factor beta ( TGF - beta ) and interleukin-10 ( IL-10 ) were measured using enzyme - linked immunosorbent assay ( ELISA ) kits . - pubmed
difficile carriage and chemotherapy regimen , antibiotic drug use , or immunosuppressive mediators , such as prostaglandin E2 ( PGE2 ) , transforming growth factor beta ( TGF - beta ) , or interleukin-10 ( IL-10 ) . - pubmed
Septin6 and Septin7 GTP binding proteins regulate AP-3- and ESCRT - dependent multivesicular body biogenesis . - pubmed
Septins ( SEPTs ) form a family of GTP - binding proteins implicated in cytoskeleton and membrane organization , cell division and host / pathogen interactions . - pubmed
Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role ( relative to ACK2 and PAT1 in mitochondria ) in producing acetate under dark , anoxic conditions and , surprisingly , also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity . - pubmed
The strains were cultured on chocolate agar plates in a 5 % CO2 atmosphere at 37 C and identified by colony morphology , Gram staining and oxidase reaction , followed by carbohydrate utilization test . - pubmed
Anti - nociceptive effects of GL were assessed by using acetic acid - induced writhing , hot plate test and formalin test , as well as evaluation of spontaneous locomotor activity and motor performance . - pubmed
The results showed that GL significantly reduced xylene - induced ear edema , carrageenan - induced paw edema , and acetic acid - induced vascular permeation . - pubmed
Additionally , GL significantly inhibited the nociceptions induced by acetic acid and formalin . - pubmed
The CCKA - receptor requires the tyrosyl residue in the C - terminal bioactive site of CCK to be O - sulfated , whereas , the CCKB - receptor binds irrespective of sulfation . - pubmed
BACKGROUND : Dietary factors such as high - sodium or high - fat ( HF ) diets have been shown to induce a proinflammatory phenotype . - pubmed
[ ( 18 ) F ]- FET PET was quantitated by calculation of maximum and mean standardized uptake values within a universal volume - of - interest ( VOI ) corrected for healthy background ( SUVmax / BG , SUVmean / BG ) . - pubmed
Linezolid use was a risk for mortality in patients with APACHE II scores of & gt;=14 . - pubmed
Reduced actions of NO and brain hypoperfusion trigger increased production of amyloid - beta that inhibits endothelial function , thus establishing a vicious cycle for impairing brain circulation . - pubmed
In contrast , the Y146S / Y154S dimerization - defective mutant displays a severe dNTPase defect in vitro , but is indistinguishable from WT in its ability to deplete cellular dNTP pools and to restrict HIV replication . - pubmed
The effect of H2O2 initial concentration and dosage on IMZ degradation rate , average oxidation state ( AOS ) , organic acid concentration , oxidation , and mineralization percentage after photo - Fenton process was determined . - pubmed
The effect of the oxidation degree , limited by H2O2 concentration and dosage , on the percentage of mineralization obtained after the biological treatment was determined and explained in terms of changes in AOS . - pubmed
We have paired the gfap promoter region with the glutamate reporter molecule , iGluSnFR , to drive expression in glial cells throughout the nervous system . - pubmed
Tg(gfap : iGluSnFR ) is expressed on the glial membrane of Muller glia cells in the retina , which rapidly respond to stimulation and the release of extracellular glutamate . - pubmed
As glial cells are associated with most , if not all , synapses , Tg(gfap : iGluSnFR ) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system . - pubmed
Cryogenian cap carbonates that overlie Sturtian glacial deposits were formed during a post - glacial transgression . - pubmed
Here , we describe microfossils from the Kakontwe Formation of Zambia and the Taishir Formation of Mongolia - both Cryogenian age , post - Sturtian cap carbonates - and investigate processes involved in their formation and preservation . - pubmed
Effect of the GSTM1 genotype on the biomarkers of exposure to polycyclic aromatic hydrocarbons : Meta - analysis . - pubmed
The role of glutathione S - transferase Mu 1 ( GSTM1 ) in the biomonitoring of polycyclic aromatic hydrocarbons ( PAHs ) is not clear . - pubmed
Our purpose has been to evaluate the influence of GSTM1 genotypes on 1 - hydroxypyrene ( 1 - OHP ) , deoxyribonucleic acid ( DNA ) adducts , and micronucleus frequency in both occupational and non - occupational populations of null and active GSTM1 carriers . - pubmed
In the non - occupationally exposed general population , 1 - OHP and micronucleus frequency were significantly higher in the null GSTM1 carriers . - pubmed
In conclusion , GSTM1 genotypes may affect the urinary 1 - OHP in the non - occupationally exposed general population , and micronucleus frequency in both occupational workers and non - occupational population . - pubmed
How the resulting amino - acid substitutions impact PrPC and PrPSc structure and propagation is poorly understood . - pubmed
Simultaneous measurement of salivary cortisol and alpha - amylase : Application and recommendations . - pubmed
Salivary cortisol ( sCort ) and salivary alpha - amylase ( sAA ) constitute proxy measures of the two major stress response systems , i.e. - pubmed
The combination of BRAF inhibitor dabrafenib with MEK inhibitor trametinib has shown its superiority to single agent therapy and is characterized by a tolerable spectrum of adverse events which shows a decrease in incidence over time on treatment . - pubmed
Analysis of the 48 h absorption kinetics of lutein showed that the time to peak level of lutein ( 12 h ) was the same for SAF and MDG groups , but the change in plasma lutein at 12 and 48 h were 129 and 320 % higher , respectively , for MDG compared with SAF . - pubmed
The strongest QTL was associated with flower color and localized to a SNP in the anthocyanin biosynthesis pathway gene , dihydroflavonol-4 - reductase ( DFR ) . - pubmed
PxylOR41 was tuned to two minor pheromone components Z9 - 14 : Ac , Z9 - 14 : OH , and their analog Z9 - 14 : Ald . - pubmed
A 22 - amino acid COOH - terminal cytoplasmic tail containing a potential site for phosphorylation by protein kinase A was also found . - pubmed
At long wavelengths the Lewis acid complex is exclusively excited ; within the complex , one enantiotopic face of the aromatic aldehyde is efficiently shielded . - pubmed
Curcumin ( diferuloylmethane ) , well known for its anti - inflammatory potential , was administered through intranasal route 1 h before LPS and OVA ( ovalbumin ) exposure to evaluate its efficacy against airway structural changes . - pubmed
Hydroxyproline level and masson 's trichrome staining were used to evaluate collagen deposition in lung . - pubmed
Adenylyl cyclases ( ACs ) catalyze the formation of the second messenger cAMP from ATP . - pubmed
Using an AC - specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N - terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro . - pubmed
Role of Insulin - Mediated Antinatriuresis in Sodium Homeostasis and Hypertension . - pubmed
Herein , the BSR activities and SMP generation / utilization were simultaneously investigated under different conditions , i.e. - pubmed
Aromatase inhibitor and tamoxifen use and the risk of venous thromboembolism in breast cancer survivors . - pubmed
The aim of this study is to examine VTE risk following long - term use of aromatase inhibitor ( AI ) compared with tamoxifen use among breast cancer survivors . - pubmed
Genetically predicted higher serum LA was associated with lower diabetes ( odds ratio ( OR ) 0.97 per percentage in total fatty acid increase in LA , 95 % confidence interval ( CI ) 0.96 to 0.99 ) and lower lipids ( low - density lipoprotein , high - density lipoprotein , and total cholesterol ) , but may be associated with higher diastolic blood pressure . - pubmed
The molecular mass determined by SDS - PAGE in the absence or presence of beta - mercaptoethanol and ESI - MS were 16.63 kDa and 18.362 kDa , respectively . - pubmed
18F - DCFPyL PET / CT scans were reviewed for sites of disease and compared to conventional imaging . - pubmed
18F - DCFPyL PET / CT detected 29 sites of metastatic disease and 3 primary tumors . - pubmed
The detection rates of conventional imaging and 18F - DCFPyL PET / CT for identifying sites of disease were 66.7 % and 88.9 % , respectively . - pubmed
AIM : The aim of the present study was to compare and evaluate the flexural strength of heat - polymerized Lucitone 199 and SR Ivocap denture base resin materials which uses polymerization techniques of compression molding and injection molding respectively and effect of artificial saliva and distilled water on long - term . - pubmed
The higher flexural strength was seen in SR Ivocap compared to that of Lucitone199 with the p value less than 0.05 which showed significant statistically . - pubmed
In overweight , the area under curve ( AUC ) for FT3 and postprandial sugar showed 0.621 and 0.531 with cutoff values of 2.02 pg / ml and 147.5 mg / dl , respectively , whereas for aspartate aminotransferase / alanine aminotransferase ( De Ritis ratio ) , the AUC was 0.583 . - pubmed
As regards , obese diabetics and the AUC for insulin and homeostatic model assessment IR were 0.657 and 0.709 , respectively , with cutoff values of 16.06 mIU / L and 7.274 , respectively , and for postprandial sugar 0.727 , in the same group ( obese ) with cutoff value of 208.5 mg / dl . - pubmed
OBJECTIVE : Tofacitinib is an oral JAK inhibitor for the treatment of rheumatoid arthritis ( RA ) . - pubmed
In subsequent multivariable analyses , baseline age , hypertension , and the total cholesterol to high - density lipoprotein ( HDL ) cholesterol ratio remained significantly associated with risk of MACE . - pubmed
After 24 weeks of treatment , an increase in HDL cholesterol and a decrease in the total to HDL cholesterol were associated with decreased MACE risk ; changes in total cholesterol , low - density lipoprotein ( LDL ) cholesterol , and disease activity measures were not . - pubmed
CONCLUSION : In this post hoc analysis , after 24 weeks of tofacitinib treatment , increased HDL cholesterol , but not increased LDL cholesterol or total cholesterol , appeared to be associated with lower future MACE risk . - pubmed
All doses of galloyl - HHDP - glucose inhibited LPS - induced JNK and NF - kappaB activation . - pubmed
Exclusion criteria were allergy to pregabalin ; calcium channel blockers , antiepileptic drugs , antidepressant drugs , any analgesics , sedatives , or oral hypoglycemic agents . - pubmed
Chronic treatment with apelin , losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function . - pubmed
Determination of four priority polycyclic aromatic hydrocarbons ( 4PAHs ) by GC - MS in traditional Turkish yoghurts . - pubmed
Therefore , the concentration and types of four polycyclic aromatic hydrocarbons were determined by gas chromatography - mass spectrometry ( GC - MS ) . - pubmed
Reversal of NOR deficits by systemic S18841 was prevented by the NMDA receptor antagonist , CPP ( 20 mg / kg ) , and the glycine modulatory site antagonist , L701,324 ( 10 mg / kg ) . - pubmed
The Impact of Aerobic Exercise and Badminton on HDL Cholesterol Levels in Taiwanese Adults . - pubmed
Elevated levels of high - density lipoprotein cholesterol ( HDL - C ) have been associated with a decreased risk of coronary heart disease ( CHD ) . - pubmed
Mitochondrial mass and mitochondrial DNA ( mtDNA ) content were significantly increased with the upregulated expression of mitochondrial biogenesis regulator PGC1 - A upon DE differentiation , accompanied by the rise of the amount of ATP ( 2.5 - fold ) and its by - product reactive oxygen species ( 2.0 - fold ) . - pubmed
Here we analyzed the basal CpG methylation of 11 KRAS - mutant and dependent pancreatic cancer cell lines and observed strikingly similar methylation patterns . - pubmed
This study presents the performance of a hybrid constructed wetland ( Bp(VF + HF)2:1 ) system which consists of an unsaturated vertical flow ( VF ) unit followed by a saturated down - flow unit simulating horizontal flow ( HF ) with HF / VF area ratio of 0.5 and influent bypass to the HF unit . - pubmed
Treating synthetic wastewater simulating municipal wastewater , optimum total nitrogen ( TN ) removal ( 57 % ) was reached at 39 % bypass and surface loading rate ( SLR ) of 33 g BOD5 / m2 day and 9.7 g TN / m2 day ( overall system ) . - pubmed
Overall greenhouse gas emissions were 0.11 ( N2O ) and 0.41 ( CH4 ) g / m2 day which corresponded to emissions factors ( relative to total organic carbon and TN influent ) of 0.7 % ( N2O ) and 3.6 % ( CH4 ) . - pubmed
Compared with a similar system with a different HF / VF area ratio of 2.0 , organic matter and nitrogen removal efficiency was similar , but surface removal rates were about 3 times higher . - pubmed
The results showed that the LPS - induced mammary histopathological changes and MPO activity were attenuated by piperine . - pubmed
LPS - induced inflammatory cytokines TNF - alpha andIL-1beta were also inhibited by piperine . - pubmed
Furthermore , LPS - induced NF - kappaB activation was suppressed by the treatment with piperine . - pubmed
In addition , we found piperine dose - dependently increased the expression of PPARgamma . - pubmed
All of these results suggested that piperine had protective effects against LPS - induced mastitis and that the mechanism may be mediated through the activation of PPARgamma . - pubmed
In this study , we identified a novel regulatory phosphorylation site ( Ser-262 ) on OX1R that abolished its capability to interact with GRK2 , but did not affect its interaction with G proteins , GRK5 , or beta - arrestin1 / 2 activation , indicating that Ser-262 is a key amino acid for OX1R internalization that contributes to induction of GRK2 - dependent biased signaling via orexin A. - pubmed
P2X7 receptors become activated under pathological conditions of ATP release like hypoxia or cell destruction . - pubmed
We found that hP2X7 receptors become activated by ATP within a few milliseconds and are permeable only to cations . - pubmed
Petroleum - derived ( petro-)polymers such as polyethylene ( PE ) , polyethylene terephthalate ( PET ) , polyurethane ( PU ) , polystyrene ( PS ) , polypropylene ( PP ) , and polyvinyl chloride ( PVC ) are extremely recalcitrant to natural biodegradation pathways . - pubmed
Among the several alcohol dehydrogenases , PQQ - dependent enzymes are mainly found in the alpha , beta , and gamma - proteobacteria . - pubmed
Type I ADHs are localized in the periplasm and contain one Ca2 + - PQQ moiety , being the methanol dehydrogenase ( MDH ) the most representative . - pubmed
In recent years , several lanthanide - dependent MDHs have been discovered exploding the understanding of the natural role of lanthanide ions . - pubmed
Type II ADHs are localized in the periplasm and possess one Ca2 + - PQQ moiety and one heme c group . - pubmed
Finally , type III ADHs are complexes of two or three subunits localized in the cytoplasmic membrane and possess one Ca2 + - PQQ moiety and four heme c groups , and in one of these proteins , an additional [ 2Fe-2S ] cluster has been discovered recently . - pubmed
From the bioinorganic point of view , PQQ - dependent alcohol dehydrogenases have been revived recently mainly due to the discovery of the lanthanide - dependent enzymes . - pubmed
Here , we review the three types of PQQ - dependent ADHs with special focus on their structural features and electron transfer processes . - pubmed
ADH - I have a Ca - PQQ or a Ln - PQQ , ADH - II a Ca - PQQ and one heme - c and ADH - III a Ca - PQQ and four hemes - c. This review focuses on their structural features and electron transfer processes . - pubmed
It is suggested that the effectiveness of pyridostigmine pretreatment is due to the carbamoylation of a portion of the tissue acetylcholinesterase , which protects it against irreversible inhibition by soman : after poisoning spontaneous decarbamoylation produces sufficient free acetylcholinesterase to restore normal function . - pubmed
[ Role of cyclic adenosine-3 ' , 5 '- monophosphate in the regulation of histidase in the skin in the process of ontogeny ] . - pubmed
Concentration of urocaninic acid in skin correlated with the histidase activity . - pubmed
Content of urocaninic acid in skin and the histidase activity were altered under effect of administration of cylic-3 ' , 5 '- AMP , dibutyryl cyclic-3 ' , 5 '- AMP , glucagon , sodium fluoride , theophylline , actinomycin D and cycloheximide . - pubmed
The plasma and pituitary growth hormone and thyrotrophic hormone levels were also determined , as well as the thyroidal 127I content and the plasma PBI . - pubmed
Results are discussed in terms of the possible relationships between thyroid hormone and GH economy during a critical developmental period . - pubmed
Immunoreactivity was greatly enhanced by pretreatment of tissue with hyaluronidase , presumably due to removal of hyaluronan bound to the HABP , and was abolished on treatment with hyaluronan hexasaccharide , presumably due to inhibition of HABP - antibody interaction . - pubmed
Calcium - dependent cell - cell adhesion among embryonic chick neural retina cells is mediated by N - cadherin , a 130,000 - Da integral membrane protein . - pubmed
We have reported that the ability of chick neural retina cells to form calcium - dependent cell - cell adhesion is also correlated with the presence of an N - acetylgalactosaminyltransferase at the cell surface ( Balsamo , J. , and Lilien , J. - pubmed
Antibodies prepared against N - cadherin precipitate the transferase containing complexes from neutral detergent extracts ; however , when the complexes are dissociated by treatment with sodium dodecyl sulfate prior to immunoprecipitation , only N - cadherin is precipitated . - pubmed
Similarly , anti - transferase antibodies immunoprecipitate N - cadherin containing complexes prior to disruption of the complex with sodium dodecyl sulfate . - pubmed
Catalysis of the transferase reaction in the complex results in the transfer of N - acetylgalactosamine phosphate to oligosaccharide chains on N - cadherin . - pubmed
Glycosylation of N - cadherin by the transferase also occurs in situ ; labeling of retina cells with [ 32P ] orthophosphate results in N - cadherin molecules containing terminal N - acetylgalactosamine linked to an oligosaccharide chain via a phosphodiester bond . - pubmed
Initially , unoccupied PR was isolated from cytosol extracts by steroid affinity chromatography , followed by chromatography on diethylaminoethyl Bio - Gel . - pubmed
The partially pure ( 3 - 15 % ) PR consisted of two steroid - binding components that migrated at 89 kDa and 109 kDa in reducing sodium dodecyl sulfate gels after being photoaffinity labeled with the synthetic progestin [ 3H]R5020 . - pubmed
Highly purified ( 30 - 70 % ) PR migrated as 93 kDa and 119 kDa progestin - binding proteins in sodium dodecyl sulfate gels . - pubmed
Synaptosomes were labeled with ( 3H ) 5 - HT and superfused with physiological buffers containing selective concentrations of a type A ( clorgyline ) or a type B ( deprenyl ) MAO inhibitor . - pubmed
In order to elevate cytoplasmic concentrations of ( 3H ) 5 - HT and therefore increase its chances for interaction with nerve terminal MAO , reserpine was added to the superfusion buffer . - pubmed
LHRH release is dependent on the availability of calcium , and prostaglandin E2 is a potent releaser of LHRH . - pubmed
PLA2 induced LHRH release in a dose - related manner at amounts of 2 , 10 , and 50 U. Omission of Ca ++ from the medium using EGTA eliminated the PLA2 effect . - pubmed
Indomethacin treatment increased rather than diminished the PLA2 stimulation . - pubmed
These results are interpreted as a demonstration that PLA2 has a role in the release of LHRH and that a different route of the cyclooxygenase may be involved besides the well known mediation of prostaglandin E2 . - pubmed
Allosteric nucleotide specificity of phosphorylase kinase : correlation of binding , conformational transitions , and activation . - pubmed
Comparisons are made of the abilities of 5 '- diphosphate analogs of ADP , including phosphorothioates , to activate , to bind , and to induce in the enzyme 's beta subunits conformational changes associated with activation . - pubmed
Two of eight dysfunctional C1 (-)- INH proteins were cleaved to lower mol wt forms that were not seen following the reaction of normal C1 (-)- INH with equimolar amounts , or less , of plasma kallikrein . - pubmed
When a preparation of normal C1 (-)- INH containing a homogeneous single band of C1 (-)- INH was exposed to C1 - s or kallikrein , a " doublet " form evolved in which the heaviest band was in the original position of native C1 (-)- INH ; C1 - s cleavage provided a second band of 96,000 ; and cleavage by kallikrein , a second band of 94,000 mol wt . - pubmed
The doublet form of C1 (-)- INH , which purified preparations frequently demonstrate , may be due to prior cleavage by C1 - s or kallikrein . - pubmed
The effect of RET on PG release required protein synthesis , inasmuch as it was lost in cultures maintained in media without amino acids or in a low volume of media . - pubmed
More PG was released after RET than after IL-1 or LPS , and only RET produced an effect that was evident by day 1 . - pubmed
The amount of CMP that produced the same size effect on PG release as these stimulators was below the detection level of PG protease assays . - pubmed
These inhibitors did not block another action of RET on chondrocytes , namely the inhibition of PG synthesis by RET immediately after treatment . - pubmed
The inhibitors did not act by reducing cell viability , because recovery of the rate of PG synthesis 3 days post - treatment occurred in inhibitor - treated cultures . - pubmed
These studies suggest that CMP is involved in cartilage autolysis that is stimulated by RET and IL-1 . - pubmed
Treatment of hepatocytes with colcemid or other microtubule - depolymerizing drugs reduced the half - life of tubulin from 50 to 2 h , whereas taxol , which stabilizes microtubules , increased the half - life . - pubmed
Combination of alkyl isonitriles with cytochrome a. - pubmed
A ' working ' model of the haemoglobin molecule as a receptor for 2,3 - diphosphoglycerate . - pubmed
The addition of hydrocortisone and insulin to the medium significantly shortens ( 25 + /- 2 PDL ) the lifespan of 10 day chick fibroblasts . - pubmed
Hydrocortisone and insulin have no effect on the growth kinetics or lifespan of retinal pigment cells in culture . - pubmed
Modification of membrane phospholipid structure can affect membrane viscosity , membrane - bound enzyme activity , and the distribution and lateral mobility of integral proteins . - pubmed
One hundred individual 1 - g doses of cephalothin sodium in 50 - ml minibags were prepared by each of three methods ; the PharmAide Fluid Dispensing System ( PAFDS ) , the Valleylab IV Formulator , and the Viavac vacuum unit , using 20 - g , 20 - g , and 1 - g vials of cephalothin sodium , respectively . - pubmed
Serum concentrations of total cholesterol ( TC ) , triacylglycerol ( TAG ) , high density lipoprotein cholesterol ( HDL - C ) and the subfraction HDL2 - C free fatty acids ( FFA ) and free glycerol were measured in venous blood samples drawn before exercise ( after a 12 - h fast ) , during walking and after 1 h and 24 h of recovery . - pubmed
Analytical methods were developed and validated for the determination of enloplatin ( an anticancer agent ) in plasma by reversed - phase LC and for platinum ( an elemental component of enloplatin ) in plasma , plasma ultrafiltrate ( PUF ) and whole blood by flameless atomic absorption spectrometry ( FAAS ) . - pubmed
A C18 or a cyano column was used , depending on sample matrix , with UV detection at 230 nm . - pubmed
An aliquot of plasma , PUF , or whole blood was mixed with a solution of Triton X-100 and Antifoam - B and injected into the FAAS system . - pubmed
Synthesis and structure - activity relationship of methyl - substituted indolo [ 2,3 - b ] quinolines : novel cytotoxic , DNA topoisomerase II inhibitors . - pubmed
In furtherance of our SAR study on the chemistry and antitumor activity of fused nitrogen heteroaromatic compounds , a series of linear , methyl - substituted derivatives of 5H- and 6H - indolo [ 2,3 - b ] quinolines were synthesized according to the modified Graebe - Ullmann reaction . - pubmed
To establish the relationship between the physicochemical and biological activities of indolo [ 2,3 - b ] quinolines , their lipophilic properties , cytotoxic and antimicrobial activity , and ability to induce topoisomerase II dependent pSP65 DNA cleavage in vitro were investigated . - pubmed
Disulfiram ( DS , 0.50 - 2.00 mmol / kg ) , a corresponding disulfide form , also exhibited similar protection . - pubmed
The maximum effect of DEDC was found by dosing at 0.5 hr prior to alloxan , and the effect afforded by DEDC pretreatment persisted up to 3 hr , whereas the effect of DS was exhibited when the compound was given 0.5 hr prior to alloxan . - pubmed
At 0.5 hr after injection , DEDC alone had a potent increasing ability on blood glucose in a dose - dependent manner , but DS was less potent . - pubmed
BW 284C51 and ethopropazine , inhibiting acetylcholinesterase ( AChE ) and butyrylcholinesterase ( BChE ) , respectively , strongly decrease neurite growth in a dose - dependent manner . - pubmed
However , echothiophate which inhibits both cholinesterases , does not change neuritic growth . - pubmed
Uronic - acid - rich protein ( UAP ) is a new urinary macromolecule which strongly inhibits calcium oxalate crystal formation . - pubmed
Glycosylphosphatidylinositol ( GPI)-anchored proteins are generally absent from the surface of cells that are defective in GPI biosynthesis . - pubmed
An aspartic acid residue ( Asp316 ) within the Q7b GPI anchoring signal , previously found to be essential for GPI anchor addition ( Waneck , G. L. , Stein , M. E. , and Flavell , R. A. - pubmed
Analysis by sedimentation on sucrose gradients shows that Q7b species retained in the ER are multimeric , whereas species that exit the ER are monomeric . - pubmed
Modulation of the higher - order folding of chromatin by deletion of histone H3 and H4 terminal domains . - pubmed
The ' tails ' of histones H3 and H4 were removed by light in situ trypsin digestion of the nuclei . - pubmed
Phosphorylation of Ga14p at a single C - terminal residue is necessary for galactose - inducible transcription . - pubmed
In this study , we show that phosphorylation occurs rapidly on a limited fraction of overexpressed Gal4p present in a sodium dodecyl sulfate - extractable subcellular fraction while a significant fraction remains stably unphosphorylated . - pubmed
We demonstrate that Gal4p is multiply phosphorylated at both the C and N termini , and we identify the precise locations of three sites of phosphorylation at serines 691 , 696 , and 699 . - pubmed
Serine 699 phosphorylation requires Gal4p 's DNA binding function and is influenced by the function of the RNA polymerase II holoenzyme component Gal11p . - pubmed
We have recently found that the deoxynojirimycin - derivative emiglitate , a recognized inhibitor of intestinal alpha - glucosidehydrolase activity , is a powerful inhibitor of glucose - induced insulin release . - pubmed
Treatment of freshly isolated islets with pertussis toxin ( PTX ) , which is known to inactivate the G ( i )- proteins , abolished the inhibitory effect of the alpha ( 2 )- adrenoceptor agonist clonidine on insulin release stimulated by the phosphodiesterase inhibitor IBMX in the presence of the protein kinase C activator TPA and even changed it into an increase . - pubmed
Emiglitate did not display any inhibitory action on insulin release induced by these secretagogues . - pubmed
Similarly , clonidine - induced inhibition of glucose stimulated insulin release was reversed by PTX . - pubmed
However , PTX did not influence the suppressive action of emiglitate on glucose - induced insulin secretion . - pubmed
In contrast , the adenylate cyclase activator forskolin totally abolished the inhibitory effect of emiglitate , but not that of the glucose analogue mannoheptulose , on glucose - induced insulin release . - pubmed
Moreover , the stimulatory effect of forskolin and cholera toxin ( CTX ) ( activator of G ( s )- proteins ) on the secretion of insulin was markedly enhanced in the presence of emiglitate . - pubmed
In conclusion , our results suggest that the inhibitory effect of emiglitate on glucose - induced insulin release is not directly related to the G ( s )- proteins , but most likely exerted solely through the selective suppression of lysosomal aglucosidehydrolase activity , a step in between the proximal and the distal G ( i )- proteins , in glucose induced stimulus - secretion mechanisms . - pubmed
Our data also suggests that the inhibitory action of emiglitate on glucose stimulated insulin release can be compensated for by an increased sensitivity of the cyclic AMP - protein kinase A pathway . - pubmed
Hence , emiglitate might indirectly elicit an increased activity of the G ( s )- proteins to facilitate the secretory process . - pubmed
To obtain evidence that mAbs in each of these preliminary clusters detected the same antigen , they were tested for their capacity to compete with each other for binding to a target cell and the molecular weights of their antigens were estimated after immunoprecipitation . - pubmed
Most preliminary clusters in the non - lineage panel contained control mAbs that had been characterised in one of the previous workshops , and this study therefore increased the number of mAbs available to each of these non - lineage antigens . - pubmed
An additional cluster , which did not fit into any other panel , contained mAbs specific for an antigen restricted to the erythroid lineage and received the nomenclature BoWC15 ( ANA8 , IL - A135 , IL - A137 , IL - A138 and IL - A160 ) . - pubmed
Subplantar injection of the non isoform- selective inhibitors of nitric oxide ( NO ) synthase , N ( G )- nitro - L - Arginine methyl ester and N ( G )- methyl - L - Arginine , and of a scavenger of NO , haemoglobin , inhibited the early phase of oedema development . - pubmed
Inhibition of cyclooxygenase activity by indomethacin reduced the late increase in paw volume after the injection of zymosan - activated plasma . - pubmed
Methylene blue , an inhibitor of the soluble guanylate cyclase , had no effect . - pubmed
Suppression of plasma cholesteryl ester transfer protein activity in acute hyperinsulinemia and effect of plasma nonesterified fatty acid . - pubmed
Cholesteryl ester transfer protein ( CETP ) is a major determinant of the plasma high - density lipoprotein cholesterol ( HDL - C ) level and plays an important role in the reverse cholesterol transport system . - pubmed
CETP activity was determined as the transfer of radiolabeled cholesterol in HDL3 to acceptor lipoprotein . - pubmed
Regression analysis showed that this suppression was correlated with plasma nonesterified fatty acid ( NEFA ) levels after the clamp and with the magnitude of the NEFA decrease ( r = .318 , P & lt ; .02 and r = .292 , P & lt ; .05 , respectively ) . - pubmed
The crystal structure of recombinant enzyme complexed with beta - cyclodextrin was refined at 2 . - pubmed
[ Nitric oxide and hemoglobin ] . - pubmed
Nitric oxide ( NO ) is produced by nitric oxide synthases ( cNOS and iNOS ) in endothelial cells upon stimulation by various agents like Ca ( 2 +)-calmodulin , cytokines and TNF . - pubmed
It acts as a paracrine on adjacent cells to activate soluble guanylyl cyclase in the production of cGMP , a second messenger in signal transduction cascades , leading to various cellular responses . - pubmed
This homeostasis of NO in the rapidly moving blood must be maintained by a delicate balance between its production by NOSs and its instant scavenging by hemoglobin ( Hb ) in the erythrocytes . - pubmed
Upon binding NO to the alpha - subunbits , Hb shifts its conformation to a T-(low - affinity extreme ) state and its beta - subunits become an efficient O2 carrier . - pubmed
The same molecular mechanism of NO - induced conformation change operates in both Hb and soluble guanylyl cyclase . - pubmed
This bond cleavage mechanism allows Hb to survive as an effective O2 carrier even after sequestration of NO . - pubmed
The NO sequestered in Hb is eventually oxidized aerobically to NO3 - in the reaction of Fe - NO + O2 - ->Fe (+ ) + NO3 . - pubmed
Met Hb ( Fe + ) so formed is cycled back to deoxy Hb ( Fe ) by intra - erythrocyte Hb reductase to complete the NO scavenging . - pubmed
Thus , the NO in the blood acts on soluble guanylyl cyclase in vascular smooth muscles to dilate the blood vessels to increase blood delivery , whereas excess NO in the blood , which is sequestered by Hb , could help Hb to deliver O2 more efficiently in peripheral tissues . - pubmed
This was consistent with removal of the signal peptide from the chitinase and an ER localization motif ( KDEL ) at the carboxyl end of the protein . - pubmed
At the end of the experiment , mean arterial pressure ( MAP ) and heart rate at rest and in response to ganglionic blockade by intravenous hexamethonium ( 30 mg / kg ) were assessed . - pubmed
pH gradients in immobilized amidases and their influence on rates and yields of beta - lactam hydrolysis . - pubmed
Studies in neuronal cells and other cells have shown that ethanol can alter intracellular calcium levels and affect voltage and receptor - operated calcium channels , as well as G protein - mediated calcium responses . - pubmed
UTP and UDP ( 1 - 500 microM ) were equipotent in inducing concentration - dependent vasoconstriction , unaffected by the P2 receptor antagonists suramin ( 100 microM ) and Reactive blue 2 ( 50 microM ) ; ATP ( 10 - 500 microM ) produced weaker vasoconstriction . - pubmed
Furthermore , the pharmacological profile of the native UTP / UDP receptor differs from that of the known P2Y2 , P2Y4 and P2Y6 recombinant receptors for pyrimidine nucleotides . - pubmed
METHODS : Mucins were extracted in a denaturing medium ( to prevent endogenous proteolysis ) and purified by caesium chloride density gradient ultracentrifugation . - pubmed
Analysis of mucin was by gel filtration , SDS - PAGE and Western blotting methods . - pubmed
We identified by SDS - PAGE a glycoprotein which co - fractionates in a caesium chloride density gradient with mucins isolated from gastrectomy specimens resected for carcinoma and peptic ulceration and which was absent from mucins of the transplant donor control group . - pubmed
This neuraminidase - sensitive glycoprotein resisted dissociation from mucin during purification in a 3.5 M CsCl density gradient but was partially separable by Sepharose 2B gel chromatography and heat treatment ( 100 degrees C , 2.0 min ) in SDS . - pubmed
Dextromethorphan ( DM ) is metabolized in the body to dextrophan ( DT ) and 3 - methoxymorphinan ( 3 - MM ) by cytochrome P450 ( CYP ) 2D6 and 3A4 , respectively , and cyclosporine ( CsA ) is a known substrate of CYP3A4 . - pubmed
Metabolic ratios of DM:3 - MM based on 24 - hour cumulative urine collection did not appear to have clinical utility in predicting CYP3A activity measured by CsA clearance . - pubmed
Selection in the presence of either the herbicide Basta or the adenosine analogue 2 '- deoxyadenosine resulted in transgenic cultures that expressed GUS and accumulated a 41 - kD protein that immunoprecipated with an ADA - specific polyclonal antibody . - pubmed
Culltures expressing ADA grew in the presence of 200 mg / l 2 '- deoxyadenosine , a concentration which completely inhibited the growth of non - transgenic cultures . - pubmed
This epitope loss was observed following either direct stimulation of protein kinase C ( PKC ) with phorbol 12 - myristate 13 - acetate ( PMA ) or after multiple receptor stimulation using a combination of the agonist N - formylmethionyl - leucyl - phenylalanine ( FMLP ) and the priming agents granulocyte macrophage - colony stimulating factor ( GM - CSF ) and platelet factor ( PF ) 4 . - pubmed
The increases and subsequent decreases in CD11b expression induced by PMA were paralleled by an increase and a decrease in PMN adhesion to CD11b - specific ligands , fibrinogen and intercellular adhesion molecule ( ICAM )- 1 . - pubmed
Sodium dodecyl sulphate - polyacrylamide gel electrophoresis ( SDS - PAGE ) and Western blot analysis showed that this epitope loss of PMN CD11b was the result of proteolytic degradation of the N - terminal region of the molecule . - pubmed
The exogenous IL-2 completely restored the inhibitory effect of xylazine in vivo on thymocyte proliferation . - pubmed
However , a minimal influence of the cytokine on the xylazine - inhibited thymocyte proliferation in vitro was observed . - pubmed
Stimulatory effect of xylazine on proliferation of thymocytes was probably mediated through alpha 2 - adrenoreceptors since it was blocked by yohimbine , an alpha 2 - adrenoreceptor antagonist . - pubmed
In material stained with the post - embedding immunogold method , approximately one - third of plasma membrane - bound gold particles labeling in axon terminals and spines is associated with asymmetric synapses , thereby representing synaptic kainate receptor subunits . - pubmed
In conclusion , these data demonstrate that kainate receptors in the striatum display a pattern of subcellular distribution different from other ionotropic glutamate receptor subtypes , but consistent with their metabotropic - like functions recently shown in the hippocampus . - pubmed
The 3 ' portion of the VIT1 message is complementary to the 3 ' end of hMSH6 mRNA , enabling the formation of RNA - RNA hybrids , which may interfere with G / T mismatch repair function . - pubmed
These results suggest that RNH-6270 is a potent AT1 receptor antagonist and has anti - proliferative effects on VSMCs from SHR , which was not seen with an ACE inhibitor . - pubmed
The growth inhibitory effect of RNH-6270 may be associated with the inhibition of growth factors via antagonism to AT1 receptors . - pubmed
Control of oxygen free radical formation from mitochondrial complex I : roles for protein kinase A and pyruvate dehydrogenase kinase . - pubmed
Phosphorylation of both subunits was enhanced by cAMP derivatives and protein kinase A ( PKA ) and was inhibited by PKA inhibitors ( PKAi ) . - pubmed
NADH cytochrome c reductase activity was decreased and superoxide production rates with NADH as substrate were increased . - pubmed
On the other hand , with protein kinase A - driven phosphorylation , NADH cytochrome c reductase was increased and superoxide production decreased . - pubmed
In light of these observations we discuss a potential model for the dual regulation of complex I and the production of oxygen free radicals by both PKA and PDH kinase . - pubmed
In the RR spectra , the nu ( Fe - CO ) bands were observed at 506 , 489 cm(-1 ) ( TCP ) , 465 cm(-1 ) ( TCP - PY ) , 458 , 437 cm(-1 ) ( TCP - TG ) and 429 cm(-1 ) ( TCP - TB ) . - pubmed
Compared with the reported nu ( Fe - CO ) frequencies of hemoproteins and their model systems , these observed values are unusually low . - pubmed
This prediction is further supported by the observation of significant 13C shieldings exhibited by TCP - CO ( delta = 202.6 ppm ) and TCP - PY - CO ( delta = 202.3 ppm ) , in comparison to hemoproteins and other heme models . - pubmed
We examined the role of histone deacetylation in cyclin Bl regulation using the histone deacetylase inhibitor trichostatin A ( TSA ) . - pubmed
Promoter activity increased only 2 - fold following TSA treatment of G(0 ) cells containing the construct pCycB(MUT - E - Box)-LUC with an E - box mutation , and a decrease in activity was detected during G(2 ) . - pubmed
TSA reduction of cyclin B1 promoter activity in G(2 ) , however , involves sequences within the first 119 bp . - pubmed
The native cholinergic receptor that mediates synaptic transmission between olivocochlear fibers and outer hair cells of the cochlea is permeable to Ca ( 2 + ) and is thought to be composed of both the alpha 9 and the alpha 10 cholinergic nicotinic subunits . - pubmed
The aim of the present work was to study the permeability of the recombinant alpha 9 alpha 10 nicotinic acetylcholine receptor to Ca ( 2 + ) , Ba ( 2 + ) and Mg ( 2 + ) and its modulation by these divalent cations . - pubmed
In the absence of Ca ( 2 + ) , the EC(50 ) for ACh was higher ( 48 microM ) than that obtained with 1.8 mM Ca ( 2 + ) ( 14.3 microM ) , suggesting that potentiation by Ca ( 2 + ) involves changes in the apparent affinity of the alpha 9 alpha 10 receptor for ACh . - pubmed
We assessed the methylation status of the CpG islands in the promoter region of the p73 gene by methylation - specific PCR in 117 glioma biopsies . - pubmed
The nuclear localization signal ( NLS ) sequence of topo II is a non - classical bipartite type containing two interdependent , positively charged clusters separated by 15 amino acids . - pubmed
Serum paraoxonase ( PON ) is a high - density lipoprotein - bound enzyme that can prevent oxidation of low - density lipoprotein by hydrolyzing lipid peroxides and thus exert an anti - atherogenic effect . - pubmed
Recent studies have suggested that glutamine(Q isoform)/arginine(R isoform ) polymorphism at position 192 of PON ( 1 ) gene is associated with macrovascular disease of type 2 diabetes mellitus ( T2DM ) . - pubmed
[ Critical survey of cortisone and ACTH therapy of bronchial asthma ] . - pubmed
Objectives in this study were : ( 1 ) to describe the effects of adrenomedullin and anti - adrenomedullin antibodies on ovarian carcinoma cell proliferation , and ( 2 ) to assess the estradiol regulation of adrenomedullin metabolism . - pubmed
Adrenomedullin mRNA expression and secretion were not under estrogen control . - pubmed
CONCLUSION : Adrenomedullin metabolism is not under estradiol control . - pubmed
Our results show that NTN expression is relatively low in the striatum , the target of dopamine ( DA ) neurons , and there is no apparent pattern of developmental regulation in SN . - pubmed
Polymorphisms in the methylenetetrahydrofolate reductase and methionine synthase reductase genes and homocysteine levels in Brazilian children . - pubmed
Hyperhomocysteinemia is a risk factor for thrombosis , and methylenetetrahydrofolate reductase ( MTHFR ) and methionine synthase reductase ( MTRR ) polymorphisms , folate , and B12 levels could contribute to plasma homocysteine ( Hcy ) variation . - pubmed
In this study , we investigated association of polymorphisms C677 T and A1298C in the MTHFR gene and A66 G in the MTRR gene with Hcy levels in children . - pubmed
The folate levels demonstrated a statistically significant decrease ( P = 0.0477 ) from the C677 T mutation in the MTHFR gene ( TT genotype ) when compared to the other groups . - pubmed
CRP peak was lower with statins ( 3.8 versus 7.0 mg / dl ; p = 0.0446 ) . - pubmed
Fibrates were only associated with lower CRP levels at admission ( 0.6 versus 0.9 mg / dl ; p = 0.0010 ) . - pubmed
The effect of statins and fibrates on CRP levels in AMI should be studied further . - pubmed
Here , we use both in vivo and in vitro studies to investigate the potential roles of two prominent factors , vascular endothelial growth factor ( VEGF ) and brain - derived neurotrophic factor ( BDNF ) , in mediating brain plasticity after treatment of stroke with atorvastatin . - pubmed
Atorvastatin increases VEGF , VEGFR2 and BDNF expression in the ischemic border . - pubmed
Numbers of migrating neurons , developmental neurons and synaptophysin - positive cells as well as indices of angiogenesis were significantly increased in the atorvastatin treatment group , compared with controls . - pubmed
Anti - BDNF antibody significantly inhibited atorvastatin - induced SVZ explant cell migration , indicating a prominent role for BDNF in progenitor cell migration . - pubmed
Antioxidant status was evaluated by measuring the red cell superoxide dismutase and catalase activity , total blood glutathione , glutathione peroxidase activity in red cell and plasma , and total antioxidant capacity ( assessed as ferric reducing antioxidant power ) in plasma . - pubmed
No significant differences were found in plasma glutathione peroxidase , protein carbonyls , and total nitrates and nitrites , red cell antioxidative enzyme activities , superoxide anion release from leukocytes , and total blood glutathione between the two groups ( p & gt ; 0.05 ) . - pubmed
Subsequent experiments have shown that TEC - tolerance is dominant and mediated by CD4 regulatory T cells ( Treg ) that are generated intrathymically by recognition of antigens expressed on TECs ( Modigliani et al . - pubmed
1996b ) , with two central propositions : ( 1 ) T cell receptor ( TCR)-dependent sorting of emergent repertoires generates TEC - specific Treg displaying the highest TCR self - affinities below deletion thresholds , thus isolating repertoires undergoing positive and negative selection ; ( 2 ) Treg are intrathymically committed ( and activated ) for a unique differentiative pathway with regulatory effector functions . - pubmed
2003 ) , which is adopted by CD4 T cells with high avidity for TEC - antigens ( Bensinger et al . - pubmed
1996a ) might add to this process , it would seem that the establishment of tissue - specific tolerance essentially stems from the " promiscuous expression of tissue antigens " by TEC . - pubmed
The first targeted therapy for lung cancer is gefitinib , an EGFR inhibitor , which was approved in several countries in 2003 . - pubmed
Decoy androgen - responsive element DNA can inhibit androgen receptor transactivation of the PSA promoter gene . - pubmed
Androgen receptors ( ARs ) in the cytoplasm are activated by binding with androgen . - pubmed
Androgen - activated ARs bind to a specific genomic DNA sequence , the androgen - responsive element ( ARE ) , and initiate gene expression at the transcriptional level . - pubmed
Even without androgen activation , ARs may have a role in androgen - refractory prostate cancer . - pubmed
After 24 hr exposure to androgens , the cells were lysed and luciferase activity measured to determine the ARE decoy inhibitory effect on the function of ARs . - pubmed
The results demonstrate that ARE decoy DNA can effectively suppress androgen - activated ARs in prostate cancer cells and indicate the potential utility of decoy DNA for developing a novel therapy for prostate cancer . - pubmed
The extensive toxin : ARF - GTP interface surface mimics ARF - GTP recognition of normal cellular protein partners , which suggests that the toxin has evolved to exploit promiscuous binding properties of ARFs . - pubmed
Five months after surgery , combination chemotherapy with TS-1 ( 100 mg / body / day , 3 weeks ) and CDDP ( 10 mg / body / day , day 1 , 8 , 15 drip infusion ) in 1 course was performed , and complete response ( CR ) was noted . - pubmed
In all cases , ( a ) intestinal metaplasia ( IM ) extension ( H&E ) , ( b ) IM phenotype ( high - iron diamine [ HID ] ) , and ( c ) Cdx2 immunohistochemical expression were histologically scored in the biopsy material obtained before and after fundoplication . - pubmed
The fraction sedimenting at 1,200 g comprised mostly intact chloroplasts and contained more than half the chlorophyll but only 5 % of the 3 - phosphoglycerate phosphatase activity present in the homogenate . - pubmed
Further purification of the 1,200 g pellet fraction on a Percoll step gradient yielded greater than 95 % intact chloroplasts , yet the phosphatase activity was reduced more than 15 - fold on a chlorophyll basis by this purification . - pubmed
When the intact chloroplast and cytoplasmic fractions of mesophyll protoplasts were separated by silicone oil filtering centrifugation , the chloroplast fraction contained more than 90 % of the chlorophyll but had less than 12 % of the 3 - phosphoglycerate phosphatase activity . - pubmed
Pancreatic blood flow was detected by intravital microscope ; malondialdehyde ( MDA ) content and superoxide dismutase ( SOD ) activity were measured in pancreatic tissues for assessment of oxidative stress and alpha - smooth muscle actin ( alpha - SMA ) expression was determined by immunohistochemical staining and RT - PCR . - pubmed
Zn ( II ) in activating concentrations ( 100 microM ) acted as an allosteric enhancer as it increased the B(max ) ( 7.1 - fold ) , the potency ( 9.9 - fold ) , the affinity ( 1.7- and 6.1 - fold in competition against agonist and inverse agonist , respectively ) , and the efficacy ( 2.5 - fold ) of CXCL1 / GROalpha . - pubmed
The activating properties of Zn ( II ) were not due to a metal ion site between the ligand and the receptor because CXCL1 / GROalpha analogs in which the putative metal - ion binding residues had been substituted-[H19A ] and [ H34A ]-acted like wild - type CXCL1 / GROalpha . - pubmed
Based on the complex action of Zn ( II ) and on the chemokine interaction for [ R208H;R212H]-ORF74 , we conclude that the extracellular end of TM - V is important for the activation of this CXC - chemokine receptor . - pubmed
CONCLUSIONS : ALA - PDT was shown to be an effective and safe therapy for the treatment of AKs of the face and scalp in skin types I - V , with an acceptable rate of recurrence over 12 months of histologically confirmed AKs of 19 % . - pubmed
Leptin mRNA expression levels in the subcutaneous and omental adipose tissues were measured using SYBR Green I quantitative real - time PCR . - pubmed
Strategies of replacing protease inhibitors with nonnucleoside reverse transcriptase inhibitors or abacavir have been shown to improve metabolic abnormalities , particularly by decreasing cholesterol and triglyceride levels , and reducing cardiovascular risk . - pubmed
Nonnucleoside reverse transcriptase inhibitors , particularly nevirapine , result in a better lipid profile with a greater increase in HDL cholesterol and in the HDUtotal cholesterol ratio , one of the most important parameters associated with a reduction in cardiovascular risk . - pubmed
Although protease inhibitor compounds as a family have been linked to metabolic and body fat alterations , new drugs such as atazanavir seem to be associated with a more favorable lipid profile . - pubmed
Macrolide antibiotics , such as clarithromycin , have in vitro efficacy against IL-8 and neutrophils , key inflammatory mediators in noneosinophilic asthma . - pubmed
Clarithromycin therapy significantly reduced airway concentrations of IL-8 and neutrophil numbers and improved quality - of - life scores compared with placebo . - pubmed
From the atoms in molecules ( AIM ) theoretical analysis , common electronic features for the planar and puckered cyclobutenyl dication derivatives were observed . - pubmed
Transcription of many genes including GDNF is directed by the cAMP responsive element ( CRE ) and its cognate transcription factor CRE binding protein ( CREB ) . - pubmed
Different classes of antidepressants as well as amitriptyline acutely increased phosphorylation of CREB , but haloperidol and diazepam did not . - pubmed
The amitriptyline - induced phosphorylation of CREB was completely blocked by U0126 [ a mitogen - activated protein ( MAP ) kinase kinase 1 inhibitor ] and genistein ( a PTK inhibitor ) , but not by inhibitors of protein kinase A , p38 MAP kinase , or Ca ( 2 +)/ calmodulin - dependent kinase . - pubmed
Amitriptyline treatment also increased the expression of luciferase reporter gene regulated by CRE elements . - pubmed
The amitriptyline - induced luciferase activity was completely inhibited by U0126 in the same as phosphorylation of CREB . - pubmed
[ Carbo - dehydrated transferrin ( CDT ) as a marker of alcohol intake : problems with analysis and interpretation . - pubmed
Apigenin - mediated dephosphorylation of Akt resulted in inhibition of its kinase activity , which was confirmed by reduced phosphorylation of proapoptotic proteins BAD and glycogen synthase kinase-3 , essential downstream targets of Akt . - pubmed
These results suggest that Akt inactivation and dephosphorylation of BAD is a critical event , at least in part , in apigenin - induced decreased cell survival and apoptosis . - pubmed
5 - Lipoxygenase inhibitors potentiate 1alpha,25 - dihydroxyvitamin D3 - induced monocytic differentiation by activating p38 MAPK pathway . - pubmed
The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP - activated ROS production . - pubmed
The levels of total cholesterol , high - density lipoprotein cholesterol , triglyceride and homocysteine were measured . - pubmed
Total cholesterol was decreased , while high - density lipoprotein cholesterol was increased in heparin compared with that in control group ( P=0.001 and 0.002 ) . - pubmed
Coincidently , the expression of MMP-2 in the atherosclerotic lesions in the heparin group was 49.3 % lower than that in the control group ( P<0.001 ) . - pubmed
Only high activity of gastritis ( neutrophil infiltration ) and low bacterial load in gastric mucosal specimens as well as drinking alcohol and the use of histamine H2 receptor blockers reduced culture efficacy in infected subjects . - pubmed
On the self - assembly of a highly selective benzothiazole - based TIM inhibitor in aqueous solution . - pubmed
In these cells , estradiol ( E(2 ) ) /ER and synthetic androgen ( R1881)/AR trans - activate EGFR leading to the downstream signaling and to ERK activation . - pubmed
Structurally , FI is a 88kDa heterodimer of a heavy chain consisting of one FI - membrane attack complex ( FIMAC ) domain , one CD5 domain and two low - density lipoprotein receptor domains ( LDLr ) , and a light chain which is a serine protease domain ( SP ) , linked to the heavy chain by a disulfide bond . - pubmed
The pathophysiology of iron - induced compromised fertility in women with thalassemia major ( TM ) was evaluated in 26 adult TM females . - pubmed
AMH correlated with non - transferrin - bound iron ( NTBI ) , suggesting a role of labile iron in the pathogenesis of decreased reproductive capacity , possibly occurring in parallel to cardiac iron toxicity , as cardiac iron was associated with the presence of amenorrhea and with NTBI levels . - pubmed
The optimal conditions for the AR induction and modulation were determined as : 2 h - intertreatment time ( in PBS , at 4 C ) given after a priming dose ( 50 microg / ml ) of zeocin treatment . - pubmed
These extracts enhanced the AR and also decreased DNA damage caused by zeocin ( after 0 , 1 and 4 h - recovery time after the test dose of zeocin application ) to more than 50 % . - pubmed
Sublethal LPS transiently increased the expression of critical components of the mitochondrial transcriptional machinery , including nuclear respiratory factor 1 ( NRF1 ) and mitochondrial transcription factor A ( TFAM ) , as well as mtDNA copy number , mitochondrial protein levels , and markers of functional mitochondria , such as increased cellular ATP content , citrate synthase activity , and maximal respiration capacity . - pubmed
Development of cross - reactive antibodies to the proline - rich region of pneumococcal surface protein A in children . - pubmed
In this study , sera of children with history of pneumococcal colonization were analyzed for presence of IgG antibodies to the conserved proline - rich region ( PRR ) of PspA. - pubmed
A new bifunctional compound NCC , which undergoes thiol - mediated disulfide cleavage after cell entry , produces a red - shifted fluorescent emission in the cytosol and releases free active DNA alkylating agent CLB into the nucleus , and finally leads to DNA damage and cell death . - pubmed
Serum free triiodothyronine and free thyroxine levels were elevated and TSH level was suppressed , suggesting the existence of hyperthyroidism . - pubmed
These results suggest that CS exposure initiates an Ag - specific response that leads to pulmonary destruction and inflammation that involves both CD8(+ ) and CD4(+ ) T cells . - pubmed
The results showed that IdeR binds 2 equiv of Fe ( II ) with dissociation constants that differ by a factor of 25 . - pubmed
The high- and low - affinity iron binding sites were assigned to physical binding sites I and II , respectively , using metal binding site mutants . - pubmed
IdeR was also found to contain a high - affinity Zn ( II ) binding site that was assigned to physical metal binding site II through the use of binding site mutants and metal competition assays . - pubmed
Together , these results suggest that IdeR is a mixed - metal repressor , where Zn ( II ) acts as a structural metal and Fe ( II ) acts to trigger the physiologically relevant promoter binding . - pubmed
CONCLUSIONS : We concluded that the atlantoaxial transarticular screw fixation and posterior fusion using polyester cable can be used for C1 - 2 fusion with a high fusion rate and less complications in various cases . - pubmed
The antithrombin ( AT ) binding properties of heparin and low molecular weight heparins are strongly associated to the presence of the pentasaccharide sequence AGA * IA ( A ( NAc,6S )- GlcUA - A ( NS,3,6S )- I ( 2S )- A ( NS,6S ) ) . - pubmed
This novel oligosaccharide was characterized by NMR spectroscopy , and its binding with AT was determined by fluorescence titration , NMR , and LC - MS . - pubmed
These results establish alterations of the gene encoding the NMDA receptor NR2A subunit as a major genetic risk factor for IFE . - pubmed
Synthesis of novel pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine derivatives : potent and selective adenosine A3 receptor antagonists . - pubmed
Compounds 2 -( 3 - fluorophenyl )- 8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5b ) , 2 -( furan-2 - yl )- 8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5d ) , and 2 -( furan-2 - yl )- 5 - methyl-8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5j ) showed high affinity for the A3 receptors , with Ki values of 8.1 , 10.4 , and 12.1 nM , respectively , and were & gt;1000 - fold selective versus all other adenosine receptor subtypes . - pubmed
Spermidine stimulates T cell protein - tyrosine phosphatase - mediated protection of intestinal epithelial barrier function . - pubmed
A previous study demonstrated that the small molecule spermidine is a selective activator of TCPTP in vitro . - pubmed
On a functional level , spermidine protected barrier function in the setting of inflammation , restricting the decrease in transepithelial electrical resistance and the increase in epithelial permeability induced by IFN - gamma in coincubation experiments . - pubmed
These data implicate spermidine as a potential therapeutic agent to treat conditions associated with elevated IFN - gamma signaling and a faulty mucosal barrier . - pubmed
Heme is a suggested limiting factor in peroxidase production by Aspergillus spp . - pubmed
In this study , the role of genes coding for coproporphyrinogen III oxidase ( hemF ) and ferrochelatase ( hemH ) was analyzed by means of deletion and overexpression to obtain more insight in fungal heme biosynthesis and regulation . - pubmed
Nevertheless , both gene deletion mutants showed an extremely impaired growth even with hemin supplementation which could be slightly improved by media modifications and the use of hemoglobin as heme source . - pubmed
Overexpression of hemH , but not hemF or the aminolevulinic acid synthase encoding hemA , modestly increased the cellular heme content , which was apparently insufficient to increase activity of endogenous peroxidase and cytochrome P450 enzyme activities . - pubmed
Second trimester thyroid - stimulating hormone , total and free thyroxine reference intervals for the Beckman Coulter Access 2 platform . - pubmed
The association between the ERCC1 / 2 polymorphisms and the clinical outcomes of the platinum - based chemotherapy in non - small cell lung cancer ( NSCLC ): a systematic review and meta - analysis . - pubmed
OBJECTIVE : To prospectively investigate the association between serum uric acid ( SUA ) level and incidence of impaired fasting glucose ( IFG ) in adult Chinese . - pubmed
Indeed , omalizumab has been reported to be effective in a number of IgE - mediated and non - IgE - mediated disorders . - pubmed
Concerning non - IgE - mediated disorders , omalizumab has been reported to be effective in nasal polyposis , autoimmune urticaria , chronic idiopathic urticaria , physical urticaria , idiopathic angioedema , and mastocytosis . - pubmed
Then P.e LPS was treated with Ca ( OH ) 2 for 30 mins or 60 mins at 37 degrees centigrade in vitro and the activity of P.e LPS was evaluated by Chromogenic End - point Tachypleus Amebocyte Lysate ( CE TAL ) test . - pubmed
P.e LPS treated with 10 % and 15 % Ca ( OH ) 2 both presented the best results by CE TAL and significant difference compared with P.e LPS control group . - pubmed
Post radiopacity was measured in millimetres of aluminium ( mmAl ) with reference to an aluminium step wedge . - pubmed
Significant inverse correlation between high - density lipoprotein cholesterol and apoA with angiographic score was identified in women ( r = -0.23 , p = 0.028 and r = -0.26 , p = 0.025 ) , but not in men . - pubmed
One - pot preparation of glutathione - silica hybrid monolith for mixed - mode capillary liquid chromatography based on " thiol - ene " click chemistry . - pubmed
A novel glutathione ( GSH)-silica hybrid monolithic column synthesized via a combination of thiol - ene click reaction and one - pot process was described , where thiol - end GSH organic monomer and 2,2 - azobisisobutyronitrile ( AIBN ) were mixed with hydrolyzed tetramethyloxysilane ( TMOS ) and gamma - methacryloxypropyltrimethoxysilane ( gamma - MAPS ) and then introduced into a fused - silica capillary for simultaneous polycondensation and " thiol - ene " click reaction to form the GSH - silica hybrid monolith . - pubmed
In addition , the GSH - silica hybrid monolith was applied to the separation of nucleotides , peptides and protein tryptic digests , respectively . - pubmed
Effects of the hydroxyl group on phenyl based ligand / ERRgamma protein binding . - pubmed
We used the implicit Molecular Mechanics / Poisson - Boltzmann Surface Area ( MM / PBSA ) method to estimate the free energies of binding for the phenyl based compound / ERRgamma systems . - pubmed
Free binding energies of BPA - D to the ERRgamma were found to be considerably less than those of BPA - A and BPA - C to the ERRgamma . - pubmed
A number of molecules have been identified as regulators of this process , including p63 ( also known as tumor protein 63 ) and Notch1 . - pubmed
Mutation of EpCAM leads to intestinal barrier and ion transport dysfunction . - pubmed
Cells with EpCAM deficiency exhibited decreased electrical resistance , increased permeability , and decreased ion transport . - pubmed
Cells with EpCAM deficiency demonstrated barrier and ion transport dysfunction . - pubmed
There is increasing evidence that local thyroid hormone ( TH ) availability changes profoundly in inflammatory conditions due to altered expression of deiodinases that metabolize TH . - pubmed
Wide - angle X - ray scattering in conjunction with pole figure technique was used to study the texture of poly ( vinylidene fluoride ) ( PVDF ) alpha and gamma phase crystals in nanolayered polysulfone / poly ( vinylidene fluoride ) films ( PSF / PVDF ) produced by layer - multiplying coextrusion . - pubmed
A large fraction of those crystals was oriented with macromolecular chains perpendicular to the PSF / PVDF interface as evidenced from the ( 021 ) pole figures . - pubmed
The gamma crystals orientation was probed with the ( 004 ) pole figures showing the c - axis of PVDF gamma crystals perpendicular to the PSF / PVDF interface . - pubmed
Herein , atom - based 3D - QSAR analysis was performed using receptor - guided alignment of 46 flavonoid inhibitors of aldose reductase ( ALR2 ) enzyme . - pubmed
6 products derived from sulfonamide S -- N bond cleavage , aniline moiety oxidation and Smiles - type rearrangement were identified , and transformation pathways of SMZ oxidation were proposed . - pubmed
Multinomial conditional logistic regressions showed that elderly individuals with T2DM and PD were more likely to have HbA1c and lipid ( HbA1c & lt ; 8 % ; LDL - C & lt;100 mg / dl ; HDL - C & gt;= 50 mg / dl ; triglyceride & lt;150 mg / dl ; and total cholesterol & lt;200 mg / dl ) control . - pubmed
The analyses resulted in several important findings : 1 ) Main components are additive and additive - by - additive effects for GCA and dominance - related effects for SCA and MPH , and additive - by - dominant effect for MPH was partly identified as additive effect ; 2 ) the ranking of factors affecting heterosis was dominance & gt ; dominance - by - dominance & gt ; over - dominance & gt ; complete dominance ; and 3 ) increasing the proportion of F1 hybrids in the population could significantly increase the power to detect dominance - related effects , and slightly reduce the power to detect additive and additive - by - additive effects . - pubmed
Here we studied the calcium channels that contribute to fast - gated BK channel activation and increased excitability of beta4 knockout neurons . - pubmed
In addition to chemical analyses in samples of effluents , surface waters , sediments , and fish , ( 1 ) dioxin - like potentials were measured in paired samples of effluents , surface waters , and sediments by use of an in vitro biotest ( reporter gene assay ) and ( 2 ) dioxin - like effects were investigated in exposed fish by use of in vivo activity of the mixed - function , monooxygenase enzyme , ethoxyresorufin O - deethylase ( EROD ) in liver . - pubmed
All advanced technologies studied , based on degradation or adsorption , significantly reduced dioxin - like potentials in samples and resulted in lesser EROD activity in livers of fish . - pubmed
We also evaluated the effect of vemurafenib in four V600 BRAF melanoma cell lines with / without MC1R variants . - pubmed
Data in vitro showed a significant different phosphorylation of Erk1 / 2 and p38 MAPK during treatment , associated with a greater increase in vemurafenib IC50 in MC1R variant cell lines . - pubmed
Therefore , the elevated catalytic activity toward the EOR at PtSn - based electrodes is mainly associated with the improved CO tolerance and the incomplete oxidation of ethanol to form acetic acid and acetaldehyde species , causing the formation of a higher amount of both C2 products with the rise of Sn loading . - pubmed
While it induced a significant increase of serum insulin ( 3.41 + - 0.08 and 3.28 + - 0.08 vs. 2.39 + - 0.27 ) , HDL - cholesterol , total antioxidant capacity levels , beta cell function percent , and pancreatic reduced glutathione ( GSH ) content ( p & lt ; 0.05 ) and improved histopathological changes of the pancreas . - pubmed
We experimentally present the radiative heat induced thermo - optic action detectable by SPR shift through imaging of a thermal source onto a bulk metal grating substrate with IR - absorptive silicon nitride coating . - pubmed
Rhein exhibits antitumorigenic effects by interfering with the interaction between prolyl isomerase Pin1 and c - Jun. The Pin1 protein ( or peptidyl - prolyl cis / trans isomerase ) specifically catalyzes the cis / trans isomerization of phosphorylated serine / threonine - proline ( Ser / Thr - Pro ) bonds and plays an important role in many cellular events through the effects of conformational change in the function of c - Jun , its biological substrate . - pubmed
Pin1 phosphorylation has been regarded as a marker of Pin1 isomerase activity , and the phosphorylation of Ser / Thr - Pro on protein substrates is prerequisite for its binding activity with Pin1 and subsequent isomerization . - pubmed
Since phosphorylation of proteins on Ser / Thr - Pro is a key regulatory mechanism in the control of cell proliferation and transformation , Pin1 has become an attractive molecule in cancer research . - pubmed
Here , we demonstrated the antitumorigenic effect of rhein using cell proliferation assay , anchorage - independent cell transformation , pull - down assay , luciferase promoter activity , fluorescence - activated cell sorting and western blot analysis . - pubmed
The rhein / Pin1 association was found to play a regulatory role in cell proliferation and neoplastic cell transformation and the binding of phosphorylated c - Jun ( Ser73 ) with Pin1 was markedly decreased and inhibited activator protein 1 or NF - kappaB reporter activity by rhein . - pubmed
Overall , our findings and the accompanying biochemical data demonstrated the antitumorigenic effect of rhein through its interference in Pin1 / c - Jun interaction and suggest the possible use of rhein in suppressing the tumor - promoting effects of Pin1 . - pubmed
Expert opinion : The phase II open label trial suggested that edaravone is safe and effective in ALS , markedly reducing 3 - nitrotyrosine levels in the cerebrospinal fluid . - pubmed
Volume overload and activation of the renin - angiotensin - aldosterone system play a major role in the pathophysiology of hypertension . - pubmed
The hydrochemistries and carbon isotopic signatures of dissolved inorganic carbon from groundwater were measured during the rainy season ( July ; 41 samples ) and post - rainy season ( October ; 26 samples ) . - pubmed
The mean contribution of atmospheric / pedospheric CO2 to the total aquatic HCO3 - decreased by 15.67 % ( rainy season ) and 14.17 % ( post - rainy season ) due to the contributions made by these acids . - pubmed
BACKGROUND : The use of GnRH analogue medication is essential in reproductive medicine to avoid premature ovulation by pituitary suppression for the duration of ovarian stimulation by gonadotrophins . - pubmed
Changing the chirality of one of the residues has consequences on the CH pi interaction , which is of CalphaH pi nature for LD , while LL shows a CbetaH pi interaction . - pubmed
Antitumor effects of metformin are a result of inhibiting nuclear factor kappa B nuclear translocation in esophageal squamous cell carcinoma . - pubmed
Metformin exerts antitumor effects in various respects , such as inhibiting inflammation , tumor growth and epithelial - mesenchymal transition ( EMT ) . - pubmed
We found that localization of NF - kappaB in the nucleus was reduced after metformin treatment . - pubmed
This suggests that metformin inhibited the activation of NF - kappaB. - pubmed
Metformin inhibited cell motility and induced E - cadherin expression . - pubmed
In conclusion , metformin showed multiple antitumor effects such as growth suppression , invasion inhibition , and control of EMT by inhibiting NF - kappaB localization on ESCC . - pubmed
The as - synthesized Ag NPs were characterized by using UV - Visible spectroscopy , X - ray diffraction and transmission electron microscopy ( TEM ) . - pubmed
The TEM micrographs have revealed that spherical shaped Ag NPs with polydisperse in size were obtained . - pubmed
Brain glucose consumption abnormalities in neuro - Behcet ' s disease : a preliminary 18F - FDG PET / CT study . - pubmed
Through an integrated analysis of RCC metabolic pathways , we showed that methylthioadenosine phosphorylase ( MTAP ) and its substrate methylthioadenosine ( MTA ) are dysregulated in aggressive RCC . - pubmed
Interestingly , we found a decrease in the protein methylation level with a concomitant increase in tyrosine phosphorylation after MTAP knockout . - pubmed
A phospho - kinase array screen identified the type 1 insulin - like growth factor-1 receptor ( IGF1R ) as the candidate with the highest upregulation in tyrosine phosphorylation in response to MTAP loss . - pubmed
IGF1R suppression by a selective inhibitor of IGF1R , linsitinib , impaired the cell migration and invasion capability of MTAP - deleted cells . - pubmed
Surprisingly , an increase in linsitinib - mediated cytotoxicity occurred in RCC cells with MTAP deficiency . - pubmed
Polyethylene Glycol Acts as a Mechanistic Stabilizer of L - asparaginase : A Computational Probing . - pubmed
BACKGROUND : L - asparaginase ( L - ASN ) is an anti - cancer enzyme therapeutic drug that exerts cytotoxicity via inhibition of protein synthesis through depletion of L - asparagine in the tumor microenvironment . - pubmed
OBJECTIVE : In this study , we attempted the modification of recombinant L - asparaginase with PEG and an integrated computational strategy to probe the PEGylation in the protein to understand the biological stability / activity imparted by PEG . - pubmed
The molecular dynamics analysis revealed intricate interactions required in the coupling of PEG to L - asparaginase to bestow stronger binding affinity of L - asparagine moiety towards L - asparaginase . - pubmed
PEG mechanistically stabilized L - asparaginase through inducing pocket modification at the receptor to adapt to the cavity . - pubmed
Thiamethoxam / clothianidin significantly up - regulated the expression of cyp17 and cyp19 genes in the testis , which ultimately led to a significant decrease in testosterone levels and a significant increase in the 17 - estradiol concentrations in plasma . - pubmed
Inspired by these views , we synthesized VO xQDs herein via a one - pot top - down ethanol - thermal method using bulk VO2 as the precursor . - pubmed
The VO xQDs showed not only oxidase- and peroxidase - like activities in ethanol as the main background solution ( ethanol - BGS ) , but also exhibited additional superoxide dismutase mimetic activity in phosphate buffer solution . - pubmed
The applicability of the VO xQDs based 3D - CS was further proved via the facile and accurate H2O2 assays in three different practical samples . - pubmed
RTT cells showed increased levels of nuclear p65 and ASC proteins , pro - IL-1beta mRNA , and NLRP3 / ASC interaction in basal condition , without any further response upon the LPS + ATP stimuli . - pubmed
Furthermore , the imaging of cellulose lattices in a C - NCF / TiO2 nanohybrid system is accomplished in the presence of Ag+ ions in a medium revealing the mode of association of C - NCFs in the system , which validates the feasibility of the presented strategy . - pubmed
The content of malondialdehyde ( MDA ) in leaf exposed to Cd was higher , and the POD and CAT activity exhibited a positive response to the low level of Cd addition ( 5 mg L-1 ) . - pubmed
Assisted by ab initio calculations , confirming that the agglomeration of oxygen vacancies significantly change the local dielectric constants of the material , the results provide direct evidence that reduced dislocations can be classified as alien matter embedded in the SrTiO3 matrix . - pubmed
Experiments with endothelial cells further confirm that a mix of flavanol - derived colonic metabolites effectively down - regulate the levels of p - p65 - NF - kappaB and the cell adhesion molecules ICAM-1 and VCAM-1 , preventing thus the increase of monocyte - endothelial adhesion induced by TNF - alpha . - pubmed
A total of 9324 unigenes were identified as differentially expressed genes ( DEGs ) , of which 5380 and 3944 genes were up- and down - regulated in the group treated with bile acids , respectively . - pubmed
Among them , ' AMPK signaling pathway ' which is critical in balancing cellular energy , was significantly enriched after bile acids activation . - pubmed
In contrast to aging kidneys , adenine - induced nephropathy showed prominent expression of the genes related to cytokines , T cell activation , and fibrosis and decreased expression of the genes implicated in transporter activity and metabolism . - pubmed
Recent NHP studies have found that ( 1 ) mixed mu opioid and nociceptin / orphanin FQ peptide receptor partial agonists appear to be safe , non - addictive analgesics and ( 2 ) mu opioid receptor- and mixed opioid receptor subtype - based medications remain the only two classes of drugs that are effective in alleviating opioid - induced adverse effects . - pubmed
The majority of studies evaluating parathyroidectomy ( PTX ) for SHPT use the criteria of parathyroid hormone level ( PTH ) > 800 pg / ml with hypercalcemia and/or hyperphosphatemia , which may be accompanied by symptoms such as bone pain and pruritis that can improve after surgery . - pubmed
, 99m - technetium - sestamibi scintigraphy with computed tomography [ SPECT / CT ] , CT , or ultrasound ) is highly variable in SHPT , SPECT / CT appears to be the most sensitive . - pubmed
Dermatan sulphate was correlated with soluble syndecan-1 , and inversely correlated with blood pressure and activated partial thromboplastin time . - pubmed
The results show that an interplay between syndecan-1 and dermatan sulphate contributes to sFlt1 induced blood pressure elevation in pre - eclampsia . - pubmed
The influence of insulin upon the metabolism of glucose by the brain . - pubmed
The increased content of stearic acid increased the density of the lipoproteins so that a larger proportion of the lipoproteins were in the LDL and a smaller proportion were in the VLDL than in lipoproteins from normal plasma and eggs . - pubmed
The peptides studied had the general formula A Leu Val - His - B. Hydrolysis at 37 degrees C and pH 2.07 occurred at the amino side of the leucine residue for all the enzymes and all the peptides . - pubmed
The preovulatory rise in the oestradiol-17beta secretion rate , the LH surge and the display of oestrus all occurred earlier , with respect to the PGF-2alpha infusion , in November than in February , suggesting a greater stimulation of folliculogenesis , and therefore a greater availability of maturing follicles , in November than in February . - pubmed
We raised high - titre antisera to two LSD - bovine serum albumin conjugates , one linked via the indole nitrogen , the other via the amide side - chain . - pubmed
The antisera were specific for different parts of the LSD molecule , as demonstrated by cross - reactivity studies with LSD , its metabolites , ergot alkoloids , and closely related compounds . - pubmed
The antisera were used to develop a double - antibody radioimmunoassay with a detection limit of about 0.4 mug of LSD per liter of unextracted urine or serum . - pubmed
From measurements of total ventilation ( ml min-1 kg-1 ) and of halothane concentrations in inspired ( F1 ) end - tidal ( FE ' ) and ' mixed - spill ' ( FS ) gases , the following parameters were calculated for 5 - min intervals from 20 to 40 min after induction ; the rate of uptake of halothane per percent inspired concentration ( Vha1 ml min-1%-1 ) and the degree of equilibrium achieved with the inspired concentration , calculated as FE'/F1 expressed as a percentage . - pubmed
We engineered four expression vectors in which a synthetic oligomer encoding gp120 residues 306 to 328 ( amino acids YNKRKRIHIGP GRAFYTTKNIIG ) from the V3 loop of the MN viral isolate was inserted at various positions within the endogenous HIV-1LAI env gene . - pubmed
Thus , calcium - calmodulin dependent cellular processes are involved in both induction of IFN and its action . - pubmed
The several patterns response to TFP and W-7 may reflect different ligand - binding sites on calmodulin . - pubmed
The level of DNA polymerase alpha activity was found to gradually decrease by 60 % ( 2.3 to 0.8 nmol of [ 3H ] dCMP incorporated / mg protein / h ) between 9- and 19 - day - old ECB . - pubmed
Analysis of DNA polymerase alpha from 11- and 19 - day - old ECB by a 10 to 30 % glycerol density gradient revealed a high molecular weight peak sedimenting near catalase ( 11.3 S ) with activity at the 11th day being approximately 3 - fold greater than activity at the 19th day . - pubmed
The data are consistent with the view that brain anoxia initially increases FFA by receptor - mediated polyphosphoinositide breakdown and that the alpha-1 adrenergic receptor is one of the receptors involved . - pubmed
The data also indicate that the fight and flight reaction is dissociated from the events that lead to FFA release , and may involve the stimulation of glutaminergic NMDA receptors . - pubmed
To investigate the mechanism involved in the PDH responsiveness to insulin in circulating lymphocytes and the role of Ca2 + and Mg2 + in this process , the PDH activity was assayed in lymphocytes combined with insulin and/or a number of substances whose mechanism of action is partially known . - pubmed
Relation of amiodarone hepatic and pulmonary toxicity to serum drug concentrations and superoxide dismutase activity . - pubmed
Specific effects of GM3 on tyrosine phosphorylation of the epidermal growth factor receptor . - pubmed
Neither GM3 nor GM1 had any affect on the binding of 125I - EGF to its cell surface receptor . - pubmed
( i ) EGF - dependent tyrosine phosphorylation of the EGF receptor and its inhibition by GM3 were also demonstrated on isolated EGF receptor after adsorption on the anti - receptor antibody - Sepharose complex , and the receptor phosphorylation was enhanced on addition of phosphatidylethanolamine . - pubmed
( ii ) Phosphoamino acid analysis of the EGF receptor indicated that the reduction of phosphorylation induced by GM3 was entirely in the phosphotyrosine and not in the phosphoserine nor phosphothreonine content . - pubmed
These results suggest that membrane lipids , especially GM3 , can modulate EGF receptor phosphorylation in vitro as well as in situ . - pubmed
The occupational groups studied were not standardized for factors known to affect bilirubin , GGT , cholesterol and HDL . - pubmed
Quaternary ammonium sulfanilamide : a membrane - impermeant carbonic anhydrase inhibitor . - pubmed
A novel carbonic anhydrase ( CA ) inhibitor , quaternary ammonium sulfanilamide ( QAS ) , was tested for potency as a CA inhibitor and for its ability to be excluded from permeating biological membranes . - pubmed
Mechanism of azide binding to chloroperoxidase and horseradish peroxidase : use of an iodine laser temperature - jump apparatus . - pubmed
The kinetics of azide binding to chloroperoxidase have been studied at eight pH values ranging from 3.0 to 6.6 at 9.5 + /- 0.2 degrees C and ionic strength of 0.4 M in H2O. - pubmed
In addition , results were obtained on azide binding to horseradish peroxidase at pD 4.36 and pH 4.56 . - pubmed
The H / D solvent isotope effects indicate proton transfer is partially rate controlling and is more important in the dissociation of azide from the enzyme - ligand complex . - pubmed
A mechanism is proposed in which hydrazoic acid binds to chloroperoxidase in a concerted process in which its proton is transferred to a distal basic group . - pubmed
The binding rate constant data , kon , can be fit to the equation kon = k3/(1 + KA/[H+ ] ) , where k3 = 7.6 X 10(7 ) M-1 S-1 and KA , the dissociation constant of hydrazoic acid , is 2.5 X 10(-5 ) M. The same mechanism probably is valid for the ligand binding to horseradish peroxidase . - pubmed
Following acid hydrolysis and diethylether extraction , phenol is separated from other urinary components within 3 minutes using the proposed capillary GC method . - pubmed
General abdominal symptoms , haematological status , basal ( BAO ) and maximal ( MAO ) hydrochloric acid secretion were studied before ( I ) and 1 year ( II ) and 14 years ( III ) after the operation . - pubmed
It was therefore concluded that histamine blocks not only the specific ACh binding site but also interacts with the ACh - channel site . - pubmed
The effects of cations on the inhibition of sodium and potassium activated adenosinetriphosphatase by beryllium . - pubmed
The characteristics of alkaline phosphatase of the cells treated or untreated with cycloheximide or actinomycin D were similar to each other ; they were heat labile and the enzyme reaction was strongly inhibited by L - homoarginine , but weakly by L - phenylalanine . - pubmed
The increase in alkaline phosphatase activity with cycloheximide has been termed a ' superactivation ' of alkaline phosphatase . - pubmed
Epithelial endocrine cells containing 5 - hydroxytryptamine , substance P , somatostatin , enteroglucagon and vasoactive intestinal polypeptide - immunoreactivity were localized by immunocytochemistry in the mucosa of normal appendices , ileum and proximal colon , and in neurogenic appendicopathy . - pubmed
To examine the role in neurite growth of actin - mediated tensions within growth cones , we cultured chick embryo dorsal root ganglion cells on various substrata in the presence of cytochalasin B. - pubmed
On highly adhesive substrata , nerve cells were observed to extend numerous ( though peculiarly oriented ) neurites in the presence of cytochalasin , despite their lack of both filopodia and lamellipodia or the orderly actin networks characteristic of typical growth cones . - pubmed
In primary biliary cirrhosis the hepatic copper content was correlated with bilirubin and alkaline phosphatases but not with ceruloplasmin , coagulation factors , or aminotransferases . - pubmed
We conclude that the hepatic copper content and serum ceruloplasmin were remarkably constant during 3 years of observation in various liver disorders . - pubmed
Retinoids modulate cellular proliferation and mediate gene function through a series of nuclear receptors . - pubmed
In contrast to the RAR beta - expressing cells , colony formation by the RAR alpha - expressing cells was similar to the mock - transfected controls and the addition of 1 microM RA to the RAR alpha - transfected cells inhibited colony formation . - pubmed
In a preceding study , we established the tolerance and pharmacokinetic behavior of 5 - fluoro-2 '- deoxyuridine ( FdUrd ) given by the intraperitoneal ( IP ) route . - pubmed
Pharmacologic determinations of IP FdUrd and 5 - Fluorouracil ( FUra ) derived from it and LV were obtained by HPLC methods on 11 occasions . - pubmed
We conclude that IP FdUrd 3 g and LV in doses of up to 640 mg x 3 days are well tolerated . - pubmed
Since FdUrd is more potent , has an even greater hepatic clearance and shows greater potential for modulation with LV than FUra , it may be the preferred fluoropyrimidine for subsequent studies via the IP route in the treatment of carcinomas with prominent peritoneal spread . - pubmed
Analysis of the resulting drug - resistant populations revealed that cells producing Asp95 DHFR dominated with an overall frequency of 3:1 , and therefore , under these growth conditions , display a selective advantage over those producing Asn95 DHFR . - pubmed
Refolding of the soluble recombinant binding - active extracellular domain of the murine erythropoietin receptor ( sEPO - R ) was achieved with greater than 90 % recovery either from urea / NaCI ( 1.5/0.5 M ) or guanidine HCI ( 1 M ) . - pubmed
Methyl groups are sequentially eliminated as HCHO by incorporation of O2 catalyzed by DMS monooxygenase and methanethiol oxidase . - pubmed
H2O2 formed during CH3SH oxidation is destroyed by catalase . - pubmed
The inhibition was completely abolished by Sch23390 , a selective D1 antagonist ( 0.5 - 2 mg / kg ) , but partially reversed by SPD ( 1 - 16 mg / kg ) . - pubmed
Treatment of synovial cells with N - acetyl - L - cysteine ( NAC ) , an antioxidant agent , inhibited TNFalpha - induced NF - kappaB activation and transcription . - pubmed
Moreover , NAC also inhibited synovial cell proliferation induced by TNFalpha . - pubmed
In the troglitazone group , FPG and HbA(1c ) decreased significantly after the treatment ( before vs after , FPG : 10.8 + /- 2.0 mmol I(-1 ) vs 9.2 + /- 2.5 mmol I(-1 ) , p< ; 0.001 ; HbA(1c ): 9.2 + /- 1.4 % vs 8.5 + /- 1.5 % , p< ; 0.001 ) . - pubmed
Serum total cholesterol and HDL - cholesterol did not change in either group , however , serum triglyceride significantly decreased in the troglitazone group . - pubmed
In order to evaluate the relationship between primary colorectal adenocarcinomas and their metastases with regard to AgNOR proteins expression , we have performed a morphometric analysis of AgNOR silver precipitates ( AgNORs ) in advanced colorectal carcinomas ( stage III - IV ) as well as in corresponding lymph node and distant metastases . - pubmed
Therein , PGE2 and IL-1 production was noted to coincide with the onset of fever and the coagulation disorders typical of CSF . - pubmed
Red blood cells have been labeled with an anti - renin pharmacophore using the activated labeling agent Boc - Phe - His - ACHPA - Ile-6 - NH ( CH2 ) 5CO - NHS ( 4 ) and the corresponding sulfo - NHS - ester ( 5 ) . - pubmed
Inhibitory activity of labeled red cells is clearly associated with the cells themselves , and does not require prior release of an inhibitory entity : renin inhibition increases as a function of the concentration of NHS - ester used to label cells suspended in buffer , and with cell density ; on the other hand , the separated supernatant portions of the medium make only minor contributions to the observed inhibitory activities . - pubmed
The aims of this study were : ( 1 ) to analyze the effects of oral irrigation ( Water Pik Oral Irrigator ) on the clinical signs of adult periodontitis ( AP ) and on the levels of interleukin-1 beta ( IL - beta ) , prostaglandin - E2 ( PGE2 ) , interleukin-10 ( IL-10 ) and interferon - gamma ( IFN - gamma ) in GCF , and ( 2 ) to analyze the influence of the periodontitis - related IL-1 genotype ( IL-1GT ) on these variables . - pubmed
RESULTS : Oral irrigation plus ROH resulted in a significant reduction in PPD , BOP , GI and PI , as well as IL - beta levels by 7 days and PGE2 levels by 14 days , relative to ROH or no oral hygiene . - pubmed
ROH reduced GI , BOP and PI , and PGE2 levels by 14 days , but had no effect on IL-1 beta or IL-10 levels relative to no oral hygiene . - pubmed
However , limitations of this isomerization are as follows : ( i ) only N -( p - methoxyphenyl )- beta - lactams can be used , and ( ii ) transformation is less compatible with heteroatomic substituents bonded to the C3 position of the 2 - azetidinone ring . - pubmed
Acanthoxin B and C showed lower enzymatic activities than acanthoxin A ( 4.0 , 13.7 and 23.9 micromol of phospholipid hydrolyzed / min / mg protein , respectively ) . - pubmed
Other pesticides also dissolved in DMSO -- mecoprop , simazine or MCPA ( each up to 1 microM)--or dissolved in phosphate - buffered saline -- diuron ( up to 1 microM ) , isoproturon ( up to 3 microM ) , metoxuron ( up to 8 microM ) or metamitron ( up to 80 microM)--showed no concentration - related effects on cytokine production . - pubmed
There was , however , an inhibition of IFN - gamma and TNF - alpha production by simazine , metoxuron and mecoprop and of all three cytokines tested by diuron . - pubmed
MCPA ( 0.01 and 0.1 microM ) stimulated the production of TNF - alpha . - pubmed
The binding of methadone to plasma proteins was enantioselective with the active ( -)-(R ) enantiomer having the highest free fraction ( mean + /- SD : 21.2+/-7.6 % vs. 13.3+/-6.2 % for ( + ) -( S )- methadone , n = 8) . - pubmed
Recent clinical studies have documented the efficacy of trastuzumab ( Herceptin ; Genentech , Inc , South San Francisco , CA ) as a new biologically targeted therapy for erbB-2 receptor - positive forms of breast cancer . - pubmed
This mini - review highlights these recent findings and suggests the possibility that the loss of erbB-2 receptor - dependent myocyte survival pathways may create a susceptibility for the onset of heart failure in response to the cardiotoxicity of anthracycline treatment . - pubmed
Myocardial protein levels of CK isoforms ( Western blot ) , HEP levels , and CK kinetics ( ( 31 ) P MR spectroscopy ) were measured under basal conditions . - pubmed
The rank order of activity with respect to actin polymerization and release of toxic reactive oxygen species was eotaxin-3 = eotaxin = eotaxin-2 and eotaxin = eotaxin-2 & gt ; eotaxin-3 = monocyte chemotactic protein-3 = monocyte chemotactic protein-4 = RANTES , respectively . - pubmed
Is a role of phospholipase A ( 2 ) in cholesterol gallstone formation phospholipid species - dependent ? - pubmed
Phospholipase A ( 2 ) plays a role in cholesterol gallstone formation by hydrolyzing bile phospholipids into lysolecithin and free fatty acids . - pubmed
Then the effect of digestion of phosphatidylcholine by phospholipase A ( 2 ) on bile metastability was assessed by spectrophotometry and video - enhanced differential contrast microscopy . - pubmed
Addition of phospholipase A ( 2 ) caused the release of free fatty acids in a time - dependent manner . - pubmed
Therefore , the role of phospholipase A ( 2 ) in cholesterol gallstone formation depends on the phospholipid species present in bile , so that phospholipid species selection during hepatic excretion is , in part , crucial to the cholesterol stone formation . - pubmed
Neuroprotection of striatal neurons against kainate excitotoxicity by neurotrophins and GDNF family members . - pubmed
Here , we analysed the protective effects of brain - derived neurotrophic factor , neurotrophin-3 , glial cell line - derived neurotrophic factor and neurturin against the excitotoxic damage induced by kainate in striatal neurons in vitro and in vivo . - pubmed
Our results show that the decrease in the number of cultured striatal calbindin - positive neurons induced by kainate was prevented by treatment with any of these factors . - pubmed
We found that the numbers of striatal projection neurons ( calbindin - positive ) and striatal interneurons ( parvalbumin- or choline acetyltransferase - positive ) were differentially decreased after kainate lesion . - pubmed
Thymosin beta4 is the most abundant member ; it is expressed in most cell types and is regarded as the main intracellular G - actin sequestering peptide . - pubmed
Here we show that thymosin beta4 can be cross - linked to proteins such as fibrin and collagen by tissue transglutaminase . - pubmed
Thymosin beta4 is not cross - linked to many other proteins and its cross - linking to fibrin is competed by another family member , thymosin beta10 . - pubmed
We suggest that thymosin beta4 cross - linking is mediated by factor XIIIa , a transglutaminase that is coreleased from stimulated platelets . - pubmed
The effect of different grades of hydroxyethyl cellulose ( HEC ) and hydroxypropyl methllcellulose ( HPMC ) on the film - formation and taste - masking ability for ibuprofen granules was evaluated . - pubmed
Because irinotecan ( CPT-11 , Camptosar ) is a topoisomerase I inhibitor with a broad spectrum of antitumor clinical activity , we investigated its activity in relapsed or refractory non - Hodgkin ' s lymphomas ( NHLs ) . - pubmed
The purpose of this article is to outline and review the five major clinical trials of beta - blocker therapy in chronic heart failure ; the US Carvedilol heart failure Program ( USCP ) , the Cardiac Insufficiency Bisoprolol Study II ( CIBIS - II ) , the Metoprolol CR / XL Randomized Intervention Trial in chronic Heart Failure ( MERIT - HF ) , the Beta - blocker Evaluation of Survival Trial ( BEST ) and the Carvedilol Prospective Randomized Cumulative Survival trial ( COPERNICUS ) , and to aid the reader in the selection of appropriate candidates for beta - blocker therapy . - pubmed
Conformationally rigid N - acyl-5 - alkyl - L - prolyl - pyrrolidines as prolyl oligopeptidase inhibitors . - pubmed
In the N - acyl - L - prolyl - pyrrolidine type of prolyl oligopeptidase inhibitors the L - prolyl group was replaced by different 5 - alkyl - L - prolyl groups , resulting in a series of N - acyl-5 - alkyl - L - prolyl - pyrrolidines . - pubmed
Since N - amides of 5 - alkyl - L - prolines are conformationally more rigid than those of L - proline , the main objective was to make more rigid prolyl oligopeptidase inhibitors . - pubmed
Microsomal cytochrome p450 and/or p450 reductase appear to be involved in the reductive metabolism of 1,2 - benzisoxazole moiety under hypoxic as well as oxic conditions . - pubmed
Detailed kinetic analysis showed SULT1C1 was more prolific in that it was able to sulfate dopamine , tyramine , and apomorphine , which SULT1B1 was not . - pubmed
F - actin arrangement and the distribution of laminin , fibronectin , several integrins and phosphorylated Focal Adhesion Kinase ( FAK ) were studied using rhodamine - phalloidin and immunofluorescence . - pubmed
Inactivation of the toxin with 1,10 phenanthroline showed that the catalytic activity is important for the effect of jararhagin on cell migration , FAK phosphorylation and for the recruitment of alphav , but not as much for the anti - adhesive effect . - pubmed
Allergy - like reactions may occur following administration of iodinated contrast media ( CM ) , mostly in at - risk patients ( patients with history of previous reaction , history of allergy , co - treated with interleukin-2 or beta - blockers , etc . ) - pubmed
This periplasmic process requires at least 10 essential components for apo - Cyt c chaperoning , thio - oxidoreduction , and the delivery of heme and its covalent ligation . - pubmed
Previously , we have shown that overproduction of the putative heme ligation components CcmF and CcmH(Rc ) ( also called Ccl1 and Ccl2 ) can partially bypass the function of CcmI on minimal , but not on enriched , media . - pubmed
These findings indicate that , in the absence of CcmI , interactions between the heme ligation and thioreduction pathways become restricted for sufficient Cyt c production . - pubmed
We therefore suggest that CcmI , along with its apo - Cyt chaperoning function , is also critical for the efficacy of holo - Cyt c formation , possibly via its close interactions with other components performing the final heme ligation steps during Cyt c biogenesis . - pubmed
Reduction of alachlor - induced olfactory mucosal neoplasms by the matrix metalloproteinase inhibitor Ro 28 - 2653 . - pubmed
The present studies were designed to confirm this latter finding and to explore the potential role of MMPs in alachlor - induced olfactory carcinogenesis . - pubmed
The morphology of alachlor - induced olfactory tumors was similar whether or not Ro 28 - 2653 had been given ; the MMP inhibitor itself had no impact on olfactory mucosal histology . - pubmed
The proteins were digested by the action of trypsin , pepsin or chemotrypsin as revealed by gel filtration and SDS - PAGE analysis . - pubmed
Philanthotoxins are uncompetitive antagonists of Ca2 + -permeable AMPA receptors presumed to bind to the pore - forming region , but a detailed molecular mechanism for this interaction is missing . - pubmed
Together these studies provide strong evidence for a molecular mechanism by which polyamine toxins antagonize the AMPA receptor ion channel and provide the basis for rational development of uncompetitive antagonists of AMPA receptors . - pubmed
N -( 5 - chloro-1,3 - benzodioxol-4 - yl )- 7 -[ 2 -( 4 - methylpiperazin-1 - yl ) ethoxy ]- 5 - ( tetrahydro-2H - pyran-4 - yloxy ) quinazolin-4 - amine , a novel , highly selective , orally available , dual - specific c - Src / Abl kinase inhibitor . - pubmed
We report here a novel subseries of C-5 - substituted anilinoquinazolines that display high affinity and specificity for the tyrosine kinase domain of the c - Src and Abl enzymes . - pubmed
N -( 5 - Chloro-1,3 - benzodioxol-4 - yl )- 7 -[ 2 -( 4 - methylpiperazin-1 - yl ) ethoxy ]- 5 -( tetrahydro-2H - pyran-4 - yloxy ) quinazolin-4 - amine ( AZD0530 ) inhibits c - Src and Abl enzymes at low nanomolar concentrations and is highly selective over a range of kinases . - pubmed
Oxidative metabolism of polyunsaturated fatty acids through cyclooxygenases or lipoxygenases can generate various lipid peroxides and bioactive lipids , and regulate cellular proliferation , apoptosis , differentiation and senescence . - pubmed
The Se compound also significantly ( p<0.01 ) reduced microsomal lipid peroxidation and induced glutathione - S - transferase activity in liver and lung when measured after 8th and 22nd week . - pubmed
These findings will further strengthen the view on organoselenocyanate as an effective cancer chemopreventive agent against lung carcinogenesis when applied at the post - initiation phase . - pubmed
Although it is not widely recognized clinically , functional forms of hypothalamic hypogonadism are more than an isolated disruption of gonadotropin - releasing hormone ( GnRH ) drive and reproductive compromise . - pubmed
Spectrin maintains the lateral order in phosphatidylserine monolayers . - pubmed
We investigate the effect of the skeletal protein spectrin on the lateral order in dipalmitoyl phosphatidylserine monolayers spread on aqueous surfaces using grazing incidence X - ray diffraction . - pubmed
Without spectrin , the condensed lipid monolayer exhibits two - dimensional hexagonal packing , characterized by monotonic decrease in the d - spacing and increase in the degree of order with increasing surface pressure between 17 and 36 mN / m. Addition of spectrin to the aqueous subphase at high pressures preserves the monolayers structural parameters unchanged from 36 to 25 mN / m. These results demonstrate for the first time that spectrin could participate in sustaining the two - dimensional order in lipid domains through a direct interaction with phosphatidylserine species . - pubmed
Fructose feeding increased the blood pressure and levels of insulin , triglyceride and fatty acid . - pubmed
A giant solid tumor in the left maxillary sinus was detected on head CT and MRI , and this tumor was destroying the maxilla and extending into the orbit , pterygoid muscle and posterior paranasal sinuses . - pubmed
An experimental procedure was used based on selective quenching of both 1,3 - di ( 1 - pyrenyl ) propane ( Py-3 - Py ) and 1,6 - diphenyl-1,3,5 - hexatriene ( DPH ) by trinitrophenyl groups , and radiationless energy transfer ( RET ) from the tryptophans of membrane proteins to Py-3 - Py . - pubmed
In addition , the interaction of KBA and AKBA with the organic anion transporter OATP1B3 and the multi drug resistant proteins P - glycoprotein and MRP2 was evaluated using partly fluorescent - based assays . - pubmed
Neither KBA nor AKBA could be identified as substrates of P - glycoprotein . - pubmed
However , both KBA and AKBA modulated the activity of OATP1B3 and MRP2 , indicating that therapeutic relevant interactions with other anionic drugs may be expected . - pubmed
When using p - nitrophenyl - beta-1,4 - N , N '- diacetyl - chitobiose as substrate , the overall catalytic efficiency , k(cat)/K(m ) of the improved chitinase was 2.7- and 2.3 - fold higher than the average k(cat)/K(m ) of wild types at pH 3.0 and 6.0 , respectively . - pubmed
In adjusted analyses , older age was associated with less appropriate treatment ( odds ratio [ OR ] per 5 years , 0.91 ; P=.01 ) , and patients with LDL cholesterol & lt;or=130 mg / dL ( OR , 18.8 ; P<.001 ) and the low - risk group ( OR , 27.5 ; P<.001 ) were more likely to be managed appropriately compared with patients with LDL & gt;or=190 mg / dL and those at high risk . - pubmed
In vitro release of dexamethasone or bFGF from chitosan / hydroxyapatite scaffolds . - pubmed
Chitosan scaffolds containing dexamethasone ( Dex ) or basic fibroblast growth factor ( bFGF ) were developed to create alternative drug - delivery systems for possible tissue - engineering applications such as periodontal bone regeneration . - pubmed
Dex and bFGF were incorporated into scaffolds by embedding method ( solvent sorption method ) . - pubmed
The initial loading amounts were varied as 300 , 600 and 900 ng Dex per dry scaffold ( average dry weight is 3 mg ) and 50 or 100 ng bFGF per dry scaffold to a range of deliverable doses . - pubmed
However , bFGF release from all scaffolds with both loading amounts ( 50 ng or 100 ng ) was completed in 10 or 20 h. In order to prolong the release period of bFGF , composite scaffolds were fabricated in the presence of hydroxyapatite ( HA ) beads with average particle size of 40 mum . - pubmed
These results suggested that chitosan scaffolds can be suitable for Dex release ; however , the presence of HA in the chitosan scaffold is necessary to achieve the desired release period for bFGF . - pubmed
During deoxycorticosterone acetate - salt hypertension , CO was maintained , but TPR was elevated compared with the baseline period . - pubmed
Acute cholinesterase inhibition assays with carbofuran were performed on culture days 5 , 10 and 17 . - pubmed
In a 14 - day subchronic study with fenitrothion , total protein levels in the cultures were significantly lower than control values at all doses tested ( 4 , 8 and 20 mug / ml medium ) while cholinesterase activity was unaffected at all but the highest dose . - pubmed
We suggest that at high concentrations , L - serine decreases synthesis of UDP - N - acetylmuramate - L - alanine by the murC - encoded ligase , weakening the cell wall and producing misshapen cells and lysis . - pubmed
The inhibition by high L - serine is overcome in several ways : by a large concentration of L - alanine , by overproducing MurC together with a low concentration of L - alanine , and by overproducing FtsW , thus promoting septal assembly and also by overexpression of the glycine cleavage operon . - pubmed
They secrete a wide range of peptide hormones and biogenic amine serotonin and express neuronal markers such as chromogranins A , B , C ( CgA , B , C ) and neuron specific enolase ( NSE ) together with synaptophysin . - pubmed
The propensity of prostate cancer cells to undergo a transdifferentiation pathway towards NE phenotype is due to several microenvironmental conditions such as androgen depletion ( induced by LH - RH analogs or antagonists , antiandrogens , 5 - alpha - reductase inhibitors ) , ionizing - radiation therapy , adrenergic factors , increase in interleukin-6 signaling cascade . - pubmed
Aberrantly activated glutamic acid decarboxylase - independent pathway for production of GABA ( gamma - amino - butyric acid ) appears to be a constant feature of invasive NE tumors . - pubmed
Intriguingly , intermittent androgen deprivation therapy , by preventing NE differentiation , significantly reduces the risk of a rise in serum CgA levels meanwhile delaying the time of cancer progression due to hormone - independence . - pubmed
Low amounts of N - glucuronides were generated by HLM , HIM , and HKM , as well as by MLM and CLM , but not by RLM , according to the observed species specificity of this metabolic pathway . - pubmed
We identified 6 molecules consistently present on CD133 + cells : CD9 , CD29 , CD49b , CD59 , CD151 , and CD326 . - pubmed
By contrast , CD24 , CD26 , CD54 , CD66c , CD81 , CD90 , CD99 , CD112 , CD164 , CD166 , and CD200 showed a discontinuous behavior , which led us to identify cell type - specific surface antigen mosaics . - pubmed
RESULTS : Although the DP group had greater water ( p & lt ; 0.01 ) and feed ( p & lt ; 0.001 ) consumption , the DH group had greater body weight ( p & lt ; 0.5 ) and adipose tissue gain ( p & lt ; 0.001 ) , favoring higher adiposity index ( p & lt ; 0.001 ) , glucose ( p & lt ; 0.01 ) , and area under the curve in the insulin ( p & lt ; 0.001 ) and glucose ( p & lt ; 0.01 ) tolerance tests . - pubmed
OBJECTIVE : The use of technetium-99 m methylene diphosphonate single - photon emission tomography / computed tomography ( 99mTc - MDP SPECT / CT ) of the spine and sacrum has increased over the past years ; however , there is little information about the optimal clinical role and true clinical impact of this modality . - pubmed
METHODS : All 99mTc - MDP SPECT / CT examinations of the spine and sacrum performed at our large multispecialty institution between 1 January 2008 and 12 April 2012 were identified . - pubmed
RESULTS : Of the 212 99mTc - MDP SPECT / CT examinations of the spine and sacrum identified , 191 ( 90 % ) were for pain evaluation , 14 ( 7 % ) were to assess infection , and seven ( 3 % ) were to evaluate a potential malignancy . - pubmed
CONCLUSION : 99mTc - MDP SPECT / CT of the spine and sacrum is most frequently used for the diagnosis and management of pain . - pubmed
Although inflammatory nodules are more common with permanent fillers such as silicone , inflammatory nodule development following administration of temporary fillers such as hyaluronic acid and collagen has also been reported . - pubmed
Serum 25 ( OH ) D , IL-17 and IL-23 levels were detected by ELISA . - pubmed
While 25 ( OH ) D levels were not associated with radiographic bone erosions of RA , they were significantly lower in those with osteopenia and osteoporosis than in those with normal BMD ( P & lt ; 0.01 ) . - pubmed
These data suggest that disruption of chloride homeostasis in the ER disrupts the protein - folding capacity of the ER , leading to eventual neuron death . - pubmed
PURPOSE : To evaluate the efficacy of two silicone hydrogel bandage lenses ( SiHy ) on epithelial healing following photorefractive keratectomy ( PRK ) with anterior segment optical coherence tomography ( AS - OCT ) . - pubmed
The mRNA expression , protein levels and enzyme activities of key enzymes involved in taurine biosynthesis , cysteine dioxygenase ( CDO ) and cysteine sulfinate decarboxylase ( CSD ) , were analyzed . - pubmed
RB and PB showed lower MIC than AA and TBO . - pubmed
funiculosum and the results showed the increasing inhibition rate in the order of AA & lt ; TBO & lt ; PB & lt ; RB . - pubmed
The efficacy of the solution containing an anionic detergent agent ( SDS ) was shown to be generally higher than that of IPA and , after the SDS cleaning procedure , biosafety cabinets demonstrated acceptable contamination levels . - pubmed
Here , we investigated the role of IL-33 , a novel interleukin ( IL )- 1 family member , and its receptor ST2 in the analgesic effects of electroacupuncture ( EA ) on formalin - induced inflammatory pain . - pubmed
These data suggest that EA alleviates formalin - induced inflammatory pain , at least partially , by inhibiting of spinal IL-33 / ST2 signaling and the downstream ERK and JNK pathways . - pubmed
Reverse transcription - polymerase chain reaction ( RT - PCR ) revealed that TREM-1 mRNA was constitutively expressed at a low level in resting cells , and that its expression was upregulated by treatment with vitamin D3 ( VitD3 ) , but not by LPS . - pubmed
In addition , a luciferase reporter assay revealed that the serum response element ( SRE ) was involved in VitD3 - induced promoter activity , whereas the activator protein-1 ( AP-1 ) sites participated in the VitD3- and LPS - induced promoter activity . - pubmed
Of note , the CCAAT - enhancer - binding protein ( C / EBP ) site contributed not only to basal , but also to VitD3- and LPS - induced promoter activity . - pubmed
Transfection with transcription factor oligodeoxynucleotide ( ODN ) decoys indicated that transcription factors of the C / EBP and AP-1 families are likely involved in the basal , as well as in the VitD3- and LPS - induced TREM-1 transcription . - pubmed
Western blot analysis indicated that , of the members of the C / EBP family , C / EBPalpha was constitutively expressed in resting cells ; its expression was enhanced by treatment with VitD3 and was further increased by treatment with VitD3 and LPS . - pubmed
Moreover , the expression of c - Fos and c - Jun ( members of the AP-1 family ) was augmented by treatment with both VitD3 and LPS . - pubmed
Our previous study showed that the downstream of kinase 3 ( DOK3 ) is degraded during macrophage stimulation with CpG. - pubmed
In this study , we demonstrate that CpG treatment leads to ubiquitin - mediated degradation of DOK3 via interaction with an E3 ligase TNFR - associated factor 6 ( TRAF6 ) . - pubmed
We also identified the 27th amino acid ( lysine ) of DOK3 is responsible for Ly48 polyubiquitination of DOK3 . - pubmed
Furthermore , reintroduction of DOK3 ( K27R ) into DOK3 - deficient macrophages abolishes DOK3 degradation induced by CpG and suppresses the production of IL-6 and TNFalpha . - pubmed
More importantly , our study uncovers a novel role of an E3 ligase TRAF6 , namely , TRAF6 is also able to catalyse Lys 48 polyubiquitylation of target protein except for Lys 63 polyubiquitylation . - pubmed
Anti - inflammatory Effects of Poly - L - lysine in Intestinal Mucosal System Mediated by Calcium - Sensing Receptor Activation . - pubmed
Poly - L - lysine ( PL ) is a basic polypeptide identified for its role in the activation of CaSR through allosteric binding . - pubmed
As shown by the results from both in vitro and in vivo studies , the reduction of inflammatory cytokine production caused by PL was reversed by NPS-2143 pretreatment . - pubmed
A new type of siloxane core modified phthalide cardo chain based polyimide ( PI ) was successfully prepared from siloxane core dianhydride and ether linked phenolphthalein diamine moiety . - pubmed
This PI was further modified with different weight percentages of multi - walled carbon nanotubes ( MWCNTs ) to obtain MWCNT reinforced PI nanocomposites . - pubmed
Analogs of farnesyl diphosphate alter CaaX substrate specificity and reactions rates of protein farnesyltransferase . - pubmed
We characterized the reactivity of four alkyne - containing analogs of FPP with purified protein farnesyltransferase and a small library of dansylated peptides using an in vitro continuous spectrofluorimetric assay . - pubmed
The interaction of two steroidal saponins , dioscin and pseudo - protodioscin , from a botanical drug was studied for their equilibrium time and equilibrium amount by re - ligand fishing using HSA functionalized magnetic nanoparticles . - pubmed
Pigment composition of beads was determined by HPLC - MS - MS and degradation products were also analysed after storage ; betalamic acid being the major one . - pubmed
Metformin stimulates IGFBP-2 gene expression through PPARalpha in diabetic states . - pubmed
The anti - diabetic drug , metformin , exerts its action through AMP - activated protein kinase ( AMPK ) , and Sirtuin ( Sirt1 ) signaling . - pubmed
In this study , we demonstrate that metformin upregulates Igfbp-2 expression through the AMPK - Sirt1 - PPARalpha cascade pathway . - pubmed
Notably , activation of IGF-1 receptor ( IGF-1R)-dependent signaling by IGF-1 was inhibited by metformin . - pubmed
Taken together , these findings indicate that IGFBP-2 might be a new target of metformin action in diabetes and the metformin - AMPK - Sirt1 - PPARalpha - IGFBP-2 network may provide a novel pathway that could be applied to ameliorate metabolic syndromes by controlling IGF-1 bioavailability . - pubmed
GC - MS results showed that SFWP had a backbone composed of ( 1 2)-linked Glc , ( 1 2,6)-inkedGal and ( 1 3,6)-inked Man residues , which were terminated with ( 1 ) -inked Xyl and ( 1 ) -inked Ara at O-6 of ( 1 2,6)-inkedGal and ( 1 3,6)-inked Man along the main chain , in the ratio of 2.0 : 1.02 : 1.09 : 1.10 : 0.98 . - pubmed
2,3,7,8 - Tetrachlorodibenzo - p - dioxin ( TCDD ) is a persistent environmental contaminant and high - affinity ligand for the aryl hydrocarbon receptor ( AhR ) . - pubmed
Likewise , TCDD treatment increased mRNA expression of HSC activation and fibrogenesis genes , namely alpha - smooth muscle actin , desmin , delta - like homolog-1 , TGF - beta1 , and collagen type I. - pubmed
However , TCDD treatment did not exacerbate fibrosis , nor did it increase the collagen content of the liver . - pubmed
Instead , TCDD increased hepatic collagenase activity and increased expression of matrix metalloproteinase ( MMP )- 13 and the matrix regulatory proteins , TIMP-1 and PAI-1 . - pubmed
These results support the conclusion that TCDD increases CCl4 - induced liver damage and exacerbates HSC activation , yet collagen deposition and the development of fibrosis may be limited by TCDD - mediated changes in extracellular matrix remodeling . - pubmed
Incorporation of GP increased roughly 2 - to-3 - fold the phenolic compounds contents of bread . - pubmed
Breads incorporated with GP presented a richer volatile profile than CB , especially due to the presence of terpenes . - pubmed
GP added aroma compounds and phenolic antioxidants , and seemed to be an interesting approach to enhance bread bioactivity and acceptability . - pubmed
An efficient and facile protocol for the synthesis of quinazolinone - fused tetracyclic compounds through an iodine - mediated one - pot cascade reaction of 2 '- bromoacetophenones with 2 - aminobenzohydrazides or 2 - aminobenzamides is reported . - pubmed
Our recent data reveal that LPA induces matricellular protein CCN1 ( also known as Cyr61 ) expression in aortic smooth muscle cells ( SMCs ) and that CCN1 bridges LPA and integrin signaling pathways leading to SMC migration . - pubmed
Whether and how LPA regulates the transcriptional machinery of the CCN1 gene are unknown . - pubmed
In this study , we found that LPA markedly induces CCN1 mRNA expression in SMCs . - pubmed
Luciferase assays of a series of deleted , mutated CCN1 promoter - reporter gene constructs and dominant negative construct revealed the distal SRE and the proximal CRE in the CCN1 promoter are required for LPA - induced CCN1 gene expression . - pubmed
Our results imply that elevated LPA levels may trigger SMC migration and exacerbate restenosis and atherosclerotic lesions through the induced CCN1 , which communicates with a set of plasma membrane proteins and intracellular kinases . - pubmed
OBJECTIVE : To determine the effect of lipid / heparin versus saline infusion , with or without concurrent euglycemic hyperinsulinemia , on serum follicle - stimulating hormone ( FSH ) and luteinizing hormone ( LH ) . - pubmed
It was hypothesized that acutely elevated fatty acids and insulin would impair gonadotropin secretion . - pubmed
Myo - inositol ( MI ) , the precursor of the second messenger phosphoinositide ( PI ) , mediates multiple cellular events . - pubmed
Moreover , we found that concentrations of 7 - oxo - DHEA positively correlated with absolute CD4 + T cell counts , nadir CD4 + T cell values and with individuals who presented TB restricted to the lungs . - pubmed
Mitomycin C ( MMC ) has been utilized for its antifibrotic properties in nonmalignant tracheobronchial stenosis but its application is not well studied in post - lung transplant stenosis . - pubmed
B cell depletion with the anti - CD20 - antibody rituximab is widely considered treatment of choice for long - term immunotherapy in aquaporin-4 ( AQP4)-antibody positive neuromyelitis optica spectrum disorder ( NMOSD ) . - pubmed
In these cases , the IL6 ( interleukin-6 )- receptor blocking antibody tocilizumab has been suggested as an alternative . - pubmed
The results indicated that the highest retention of total phenolic content ( TPC ) and total flavonoid content ( TFC ) was obtained after drying at 80 C. Six phenolic compounds were identified and quantified by liquid chromatography . - pubmed
Pulmonary fibrosis was evaluated by Picrosirius red staining , immunohistochemistry for alpha - SMA and TGF - beta1 , and hydroxyproline assay and RT - qPCR for Col1alpha1 and Col3alpha1 . - pubmed
The recombinant FcCCD1A enzyme showed specificity for the 9,10 ( 9',10 ' ) double bond position of cyclic carotenoids to generate alpha - ionone and beta - ionone , while FcCCD1B cleaved lycopene and an acyclic moiety of delta - carotene , producing 6 - methyl-5 - hepten-2 - one . - pubmed
We hypothesized that vorinostat , a histone deacetylase ( HDAC ) inhibitor with antiangiogenic effects , would prevent acquired resistance to bevacizumab . - pubmed
Multi - DNA - Ag nanoclusters : reassembly mechanism and sensing the change of HIF in cells . - pubmed
Traditional Chinese medicine plays a cholagogic role mainly by promoting bile secretion , regulating SCP2 mRNA , FXR , BSEP and efflux transporter protein , dissolving cholesterol , promoting the relaxation of Oddi 's sphincter and changing the composition of bile , etc . - pubmed
Enhanced Electrochemical Characteristics of the Glucose Oxidase Bioelectrode Constructed by Carboxyl - Functionalized Mesoporous Carbon . - pubmed
This research revealed the effect of carboxyl - functionalization on the mesoporous carbon ( MC)-fixed glucose oxidase ( GOx ) for promoting the properties of bioelectrodes . - pubmed
Furthermore , the GOx immobilization and enzyme activity in MC - COOH increased 140.72 % and 252.74 % , leading to the enhanced electroactive GOx surface coverage of Nafion / GOx / MC - COOH electrode ( 22.92 % higher , 1.29 x 10 - 8 mol cm-2 ) than the control electrode . - pubmed
1 - Deoxydihydroceramide causes anoxic death by impairing chaperonin - mediated protein folding . - pubmed
SOCC is activated by depletion of Ca2 + stores in the sarco / endoplasmic reticulum , which communicates with plasma membrane SOCC via the Ca2 + sensor Stromal Interaction Molecule 1 ( STIM1 ) . - pubmed
Orai proteins alone can form highly Ca2 + selective SOCC channels . - pubmed
Also , members of the Transient Receptor Potential Canonical ( TRPC ) channel family are proposed to form heteromeric complexes with Orai1 subunits , forming SOCC with low Ca2 + selectivity . - pubmed
Recently , Ca2 + entry through SOCC , known as store - operated Ca2 + entry ( SOCE ) , was identified in glomerular mesangial cells , tubular epithelium , and renovascular smooth muscle cells . - pubmed
The discovery of the EML4 - ALK fusion gene in a limited subset of patients affected by NSCLC and the subsequent clinical development of crizotinib in 2011 has been an impressive milestone in lung cancer research . - pubmed
Afterward , modern tyrosine kinase inhibitors ( TKIs ) , such as ceritinib , alectinib , brigatinib , and lorlatinib , have been approved by the Food and Drug Administration ( FDA ) for the management of anaplastic lymphoma kinase ( ALK )- positive NSCLCs . - pubmed
Oyster EF resident communities were predicted to be enriched for dissimilatory nitrate reduction , nitrogen fixation , nitrification , and sulfite reductase genes . - pubmed
The expression levels of most ThSOS genes significantly changed after NaCl , PEG6000 , and abscisic acid ( ABA ) treatment in at least one organ . - pubmed
Together , these data suggest that overexpression of ThSOS3 confers salt stress tolerance on plants by enhancing antioxidant enzyme activity , improving ROS - scavenging capability , and decreasing the MDA content and lipid peroxidation of cell membranes . - pubmed
FORMATION OF H - addition radicals in adenine derivatives : part II . - pubmed
P1 - Adenosine 5 '- P2 - o - nitrobenzyl pyrophosphate ( nbzlppA ) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation . - pubmed
Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3 '- o -( o - nitrobenzyl ) phosphate . - pubmed
A trinucleotide U - A - G was converted to U - A - Gpnbzl and phosphorylated with polynucleotide kinase . - pubmed
and a beta 1 - selective ( atenolol 50 mg o.d . ) - pubmed
Resting saliva was assessed for flow rate , amylase activity and concentration of total protein , hexosamine and sialic acid . - pubmed
Stimulated saliva was assessed for flow rate , pH , buffer pH , amylase activity and concentration of total protein , Ca2 + , Mg2 + , Na + , K + , Cl - and PO4 ( 2 - ) . - pubmed
Virtually all of the CGRP - IR postganglionic neurons exhibit colocalization with somatostatin ( SOM ) , and , some of them with neuropeptide tyrosine ( NPY ) . - pubmed
[ The role of insulin on renal sodium handling and sympathetic nerve activity in overweight normotensive subjects ] . - pubmed
However , the interrelationship between insulin , obesity , renal sodium metabolism and sympathetic nerve activity in normotensive subjects ( NT ) still remains unclear . - pubmed
The present study , therefore , was undertaken to clarify the role of insulin on renal sodium handling and sympatho - adrenal function in overweight NT . - pubmed
During the two - hour clearance period , mean arterial pressure ( MAP ) , heart rate ( HR ) , endogenous creatinine clearance(CCr ) , urinary excretion of sodium ( UNaV ) , fractional excretion of sodium ( FENa ) , plasma immunoreactive insulin ( IRI ) , plasma norepinephrine concentration ( pNE ) , and plasma epinephrine concentration ( pE ) were determined . - pubmed
The stimulatory effect of albumin on luteinizing hormone - stimulated Leydig cell steroid production depends on its fatty acid content and correlates with conformational changes . - pubmed
The pH - dependent conformational changes of albumin and fragments thereof occurred in parallel with a change in the binding affinity for testosterone and pregnenolone . - pubmed
The fatty acid oleic acid and the drug suramin , only when present in a molar ligand - to - albumin ratio equal to or higher than 2 , inhibited the albumin - mediated stimulation of steroid production . - pubmed
These data show that the stimulatory effects of albumin species on LH - induced Leydig cell pregnenolone production depend on their fatty acid content and correlate with the potential of these molecules to undergo conformational changes . - pubmed
It is unknown via which mechanisms albumin exerts its stimulatory effect , but the LH action through the cyclic AMP pathway seems not to be affected . - pubmed
Inhibition of a cell surface protease after cisplatin chemotherapy . - pubmed
The active centre of GB binds the fluorescent probe 9 - aminoacridine ( 9 - AA ) enabling cells containing active GB to be visualised by fluorescent microscopy . - pubmed
It was observed that chemotherapy with cisplatin resulted in a marked decrease in cell surface GB activity and this decrease was due to the formation of an enzyme - inhibitor complex . - pubmed
Formation of a dehydroalanyl residue from S - benzylcysteine upon HF cleavage of a [ Sar1 , Cys8 ]- angiotensin II peptide resin . - pubmed
Because 5 - HT1A receptor - mediated regulation of 5 - HT synthesis appears to be mediated by somatodendritic autoreceptors on 5 - HT neurons in the midbrain raphe nuclei , the results suggest that these autoreceptors possess a large receptor reserve for agonists . - pubmed
The pituitary gonadotropin releasing capacity of ketoconazole , ( KC , p.o . - pubmed
It was found that KC resulted in a significant increase in serum LH ( from 8.94 + /- 1.05 to 17.55 + /- 1.32 U / l ) , FSH ( from 6.68 + /- 1.05 to 9.82 + /- 1.28 U / l ) , 17 - OH - progesterone ( from 4.51 + /- 0.34 to 11.35 + /- 0.68 nmol / l ) and a decrease in serum testosterone ( from 16.62 + /- 1.28 to 11.66 + /- 1.59 nmol / l ) and dehydroepiandrosterone ( from 6.56 + /- 0.39 to 4.82 + /- 0.37 mumol / l ) levels . - pubmed
Furthermore , it has been established that the amount of LH released by KC was midway between that of GnRH and CL . - pubmed
The same conditional pattern was observed for the concentration of cytochrome components , the membrane - associated oxidation of NADH and formate , and formate phenazine methosulfate ( PMS ) reductase activity ; succinate oxidase and PMS reductase activities were not exhibited by membranes under any growth condition due to the suc- mutation . - pubmed
R25PC , a spontaneous lct+ suc- partial revertant of R25 , did not exhibit the conditional pattern of R25 . - pubmed
Two distinct classes of R25 P1kc transductants were isolated that differed in both their growth response on succinate and DL - lactate and their oxidase activities . - pubmed
The TPA : B1 antibody reacted predominantly with duct cells and to a lesser extent with acinar and squamous cells . - pubmed
The anti - ischemic effects of SQ 31,765 or its relatively inactive enantiomer SQ 32,189 ( to test for effects not related to calcium channel blockade ) were determined in a model of stable angina . - pubmed
SQ 32,189 , which is a 10 - fold less potent calcium channel blocker in coronary arteries , did not affect ST - elevation ( 11.8 + /- 2.1 mV ) . - pubmed
18O isotope exchange experiments on phospholipase A2 determined by 13C - NMR : monomeric phosphatidylcholine and micellar phosphatidylethanolamine substrates . - pubmed
Phospholipase A2 - catalyzed exchange of H2 ( 18 ) O into 1 - alkyl-2 -[ 1 ( 13 ) C ] lauroyl - sn - glycero-3 - phosphorylethanolamine and into 1 - hexanoyl-2 -[ 1 - 13C ] hexanoyl - sn - glycero-3 - phosphorylcholine were examined . - pubmed
The results show that there are two types of PNMT - containing cells : those containing PNMT exclusively and those containing PNMT with two other catecholamine - synthesizing enzymes , tyrosine hydroxylase ( TH ) and aromatic L - amino acid decarboxylase ( AADC ) , but not dopamine beta - hydroxylase ( DBH ) . - pubmed
To test this hypothesis we chronically disrupted NE activity by transecting the ascending NE pathway ( ANP ) and acutely disrupted NE activity by administering the alpha - adrenergic receptor blocking agent phenoxybenzamine ( PBZ ) ; and we evaluated the effect of these treatments on the pattern of pulsatile LH secretion . - pubmed
MAGP is an acidic glycoprotein with a distinctive amino acid composition , being exceptionally rich in glutamic acid , rich in cystine , and low in glycine . - pubmed
MAGP was extractable from tissue homogenates using NaCl , urea , or guanidine hydrochloride solutions , only if a strong reducing agent was present . - pubmed
Thus , disulfide bonding is important for the strong association of MAGP with elastic fibers . - pubmed
The vasorelaxation to ANF is associated with elevations of cyclic GMP via an activation of the particulate form of guanylate cyclase . - pubmed
One , containing the closely related PD1 and PD14 genes , encodes the classical transplantation antigens . - pubmed
SBF was estimated by local clearance of 133Xenon registered by portable cadmium telluride detectors . - pubmed
Cancer risk appears to be associated primarily with low concentrations of total cholesterol and of low - density lipoprotein cholesterol but not of high - density lipoprotein cholesterol ( HDL ) . - pubmed
Significant carotene - HDL relationships were observed among black men ( r = 0.31 ; P less than 0.05 ) and among women ( r = 0.35 ; P less than 0.05 and r = 0.31 ; P less than 0.05 , black and white , respectively ) . - pubmed
Furthermore , the women also demonstrated a significant carotene - HDL2 association . - pubmed
From 128 pronuclear eggs , each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per milliliter in citrate - Locke 's solution and then transferred to the oviducts of pregnant foster mothers , 18 living fetuses developed ; from 74 eggs , each injected with 2730 cubic microns , 5 fetuses survived . - pubmed
There was a significant inverse correlation between plasma bromocriptine concentration and total BPRS score at 60 minutes post - bromocriptine administration . - pubmed
This late CCRA - induced right coronary vasoconstriction was not potentiated by beta - adrenergic receptor blockade but was abolished ( P less than 0.01 ) by alpha - adrenergic receptor blockade . - pubmed
Antibodies to three major antigens of the non - histone or saline - extractable nuclear antigen ( ENA ) complex were sought by counterimmunoelectrophoresis ( CEP ) in three groups of sera which gave different patterns in the immunofluorescence test for antinuclear antibodies ( ANA ) . - pubmed
An enzyme immunoassay ( EIA ) , specific for anti - SS - B was more sensitive than CEP and identified this antibody in 28 sera , compared with 18 for CEP . - pubmed
The increased release and intracellular concentration of BCAA was supported by decreased BCAA transaminase activity in the wounded muscle tissue . - pubmed
Non - REMS was not influenced by 3 , 30 , or 300 pmol PACAP Sleep and Tbr were not influenced by 3 or 30 pmol PACAP injected at light onset . - pubmed
The first endocrine event of puberty is an increase in the amplitude of gonadotropin pulses at night due to increased pulsatile release of hypothalamic gonadotropin - releasing hormone ( GnRH ) into the hypothalamic - pituitary portal system . - pubmed
Gonadal steroids are produced due to increased gonadotropin stimulation , and secondary sexual development follows a well - described pattern of changes . - pubmed
On the other hand , replacing Gly38 with alanine or serine results in striking decreases in kcat to 5 and 0.6 % , respectively , of the value observed for the wild - type enzyme , as well as in considerable increases in Km values . - pubmed
We conclude that Gly38 is probably required for proper function of the enzyme because it permits a high level of flexibility for the 36 - 39 peptide , which in turn allows the essential substrate - induced movement of the small domain . - pubmed
Bis azo dyes -- studies on the mechanism of complex formation with IgG modulated by heating or antigen binding . - pubmed
The mechanism of binding of azo dyes ( bis azo ) to immunoglobulin G of altered conformation , induced by heating or interaction with antigen was analysed in this work . - pubmed
The peptide loop composed of amino acids 68 - 88 originating from VL IgG domain , was selected to represent protein . - pubmed
The future of NaF should involve cyclical administration , in cautious initial dosage ( 50 - 60 mg / day ) of enteric - coated NaF , in conjunction with a potent inhibitor of resorption such as hormone replacement , bisphosphonates or calcitonin . - pubmed
Assessment of plasma renin activity ( PRA ) related to urinary sodium excretion was used to define subgroups with high ( n = 12 ) , medium ( n = 16 ) and low renin profiles ( n = 8) . - pubmed
Glucose , insulin and C - peptide responses to standard oral glucose tolerance test ( OGTT ) were measured . - pubmed
HDL - cholesterol levels were lower in the high renin group than in the low renin group ( 1.05 + /- 0.04 versus 1.26 + /- 0.09 mmol L-1 , P & lt ; 0.05 ) . - pubmed
In the amide I , clear changes in the bands at 1666 cm-1 , assigned to alpha II helices , 1659 cm-1 , assigned to alpha I and alpha II helices , and 1652 cm-1 , assigned to both alpha I helices and unordered structures , were found . - pubmed
The side chains of Tyr ( band at 1517 cm-1 ) and Phe ( band at 1498 cm-1 ) were found to change in going from BR to N. In the carboxylate region , no band was detected at 1737 cm-1 in the deconvoluted spectra that could correspond to the peak observed in the difference spectrum . - pubmed
The significant case - control difference in LDL size was independent of levels of high - density lipoprotein cholesterol ( HDL - C ) , non - HDL cholesterol ( non - HDL - C ) , triglyceride , smoking , systolic blood pressure , and body mass index , but was not significant after adjusting for the ratio of total cholesterol ( TC ) to HDL - C ( TC : HDL - C ) . - pubmed
A fragment containing C5 - C7 also blocks uptake and degradation of 125I - RAP by fibroblasts in a concentration - dependent manner . - pubmed
Binding competition experiments show that RAP , PAI-1 , and lactoferrin each inhibit the binding of the others , suggesting that at this site in LRP , RAP acts as a competitive , rather than an allosteric , inhibitor of PAI-1 and lactoferrin binding . - pubmed
Replacement of Na + with K + in the acidified medium canceled the effect of EGF . - pubmed
Indomethacin and W-7 ( a Ca - calmodulin inhibitor ) did not alter the protective effect of EGF . - pubmed
In contrast , genistein ( a tyrosine kinase inhibitor ) , amiloride ( a Na +/ H + exchangers I and II inhibitor ) , and wortmannin ( a phosphatidylinositol 3 - kinase inhibitor ) significantly decreased the effect of EGF . - pubmed
GM - CSF levels were measured by ELISA and melatonin levels by radioimmunoassay ( RIA ) . - pubmed
We also analyzed the activity of calpain I , calpain II , and calpastatin by the conventional DEAE chromatographic method for comparison . - pubmed
The results indicated that the RPA is more repeatable than the DEAE method . - pubmed
Special features of the RPA as compared with DEAE chromatography are as follows ; 1 ) the RPA is a reliable method for quantifying the expression of calpains in all tissues because it is not affected by the presence of inhibitors or activators , 2 ) the RPA method can be expanded to analyze the expression of the tissue - specific calpains simply by designing specific probes for them , and 3 ) the RPA requires a small amount of tissue . - pubmed
Mitogen - activated protein kinase and abscisic acid signal transduction . - pubmed
It was found that ABA signal transduction in plants can involve the activity of type 2C - phosphatases ( PP2C ) , calcium , potassium , pH and a transient activation of MAP kinase . - pubmed
However , type 2C phosphatases are involved in the induction of most ABA responses , as shown by the PP2C - deficient abi - mutants . - pubmed
In addition , the role of farnesyl transferase as a negative regulator of ABA responses also indicates towards involvement of MAP kinase in ABA signal transduction . - pubmed
Further establishment of the involvement of MAP kinase in ABA signal transduction and its role therein , still awaits more study . - pubmed
In one of the cell lines ( Mut F ) , two unmethylated CpG islands in the double minute chromosomes are readily cleaved by methylation - sensitive rare - cutting restriction endonucleases . - pubmed
The partially methylated CpG islands can be demethylated by azacytidine treatment or naturally by extended time in culture , and become sensitive to cleavage with the rare - cutting restriction endonucleases . - pubmed
Whole blood chemiluminescence ( CL ) , which allows for the evaluation of both the oxygen radical release by phagocytes and the serum opsonin activity using very small amounts of blood , is considered to be a useful means of assessing the host defense against infection . - pubmed
As early as 11 days after C225 treatment , the median percentage of proliferating cell nuclear antigen - positive cells was substantially reduced compared with gemcitabine treatment alone ( 26 % versus 73 % , respectively ) versus controls ( 92 % ) , correlating with in vivo blockade of EGF - R activation . - pubmed
Roles of A beta and carboxyl terminal peptide fragments of amyloid precursor protein in Alzheimer disease . - pubmed
We focused on the amyloidegenic carboxyl terminal fragments of betaAPP containing the full length of A beta ( CT105 ) . - pubmed
Our results suggest that the cytotoxic and channel inducing effects of CT105 are much more potent than that of A beta and toxic mechanisms of CT105 are different from those of A beta . - pubmed
It is known that nitric oxide ( NO ) influences the activities of glycolytic enzymes , resulting in alteration of glycolysis rate . - pubmed
RESULTS : Glibenclamide infusion had a high proarrhythmic action ( ASI : glibenclamide 143 , control 54 and vehicle 23 ; ANOVA P & lt ; 0.001 drug vs. control and vehicle ) and a detrimental effect on regional systolic ( % WTH : glibenclamide 26.9 + /- 6.7 , control 65.7 + /- 3.5 and vehicle 68.6 + /- 5.6 , ANOVA P & lt ; 0.01 drug vs. control and vehicle ) and diastolic function ( CR : glibenclamide 76.2 + /- 7.8 , control 104.7 + /- 4.2 and vehicle 106 + /- 4.9 , ANOVA P & lt ; 0.05 drug vs. control and vehicle ) during reperfusion . - pubmed
Structural requirements of sphingosine molecules for inhibition of DNA primase : biochemical and computational analyses . - pubmed
Using 28 chemically well - defined compounds containing D - erythro - sphingosine and its analogues , we analyzed structure - activity relationships for DNA primase inhibition . - pubmed
Biochemical studies demonstrated a positively charged amino group at C2 and a long aliphatic chain to be absolutely required for inhibition . - pubmed
This result could be due to cancellation of positive charge of the amino group by the interaction with negatively charged C1 - phosphate , since simulations with the software INSIGHT II showed these two groups to be close enough to interact . - pubmed
The hydroxyl group at C3 and trans - double bond at C4 - C5 were also found to be important for the inhibition . - pubmed
Despite saturation of the double bond , introduction of a hydroxyl group into C4 of dihydrosphingosine resulted in restoration of inhibition . - pubmed
Modifications such as deletion of the hydroxyl group at C3 or saturation of the C4 - C5 double bond caused shifts in the dihedral angle of C2 - 3 - 4 - 5 , with concomitant decrease in inhibitory activity . - pubmed
The AF10 gene encodes a putative transcription factor containing an N - terminal LAP / PHD zinc finger motif , a functional nuclear localization signal , an AT - hook domain , and a leucine zipper toward the C - terminus . - pubmed
The chimeric fusion proteins MLL / AF10 and CALM / AF10 , resulting from the t(10;11)(p12;q23 ) and the t(10;11)(p12;q14 ) , respectively , consistently retain the leucine zipper motif of AF10 . - pubmed
The leucine zipper interacted with GAS41 , a protein previously identified as the product of an amplified gene in a glioblastoma . - pubmed
The retention of the leucine zipper in the MLL and CALM fusions suggests that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate - dependent chromatinremodeling complexes . - pubmed
The effects of GTP gamma S could be antagonised by pre - treatment of hyphae with GDP beta S , a non - phosphorylatable form of GDP . - pubmed
This could be a dynamin - like GTP - ase , since GTP gamma S - stimulated tubule formation has only been reported previously in cases where a dynamin is involved . - pubmed
Brefeldin A antagonised GTP gamma S , and inhibited tubule formation in the tip cell . - pubmed
However , Exendin-4 treatment prevented hyperglycaemia ( fasting : 6.1+/-1.0 mmol / l , p<0.01 ) , with reduced plasma insulin concentrations ( p<0.001 ) and improved glucose tolerance ( p<0.05 ) . - pubmed
Regulation of equine lymphocyte beta - adrenoceptors under the influence of clenbuterol and dexamethasone . - pubmed
for 12 days , decreased significantly ICYP binding sites by approximately 30 - 40 % ; concomitantly , lymphocyte cAMP response to ( -)-isoprenaline was reduced . - pubmed
After withdrawal of clenbuterol , beta2 - adrenoceptor density and responsiveness gradually increased , reaching predrug levels after 4 days . - pubmed
for 5 days ) immediately after clenbuterol withdrawal accelerated beta2 - adrenoceptor recovery : only 24 h after administration dexamethasone restored the number of binding sites and cAMP response to ( -)-isoprenaline to levels statistically indistinguishable from values before clenbuterol treatment . - pubmed
Three days after dexamethasone administration , lymphocyte beta2 - adrenoceptors were further increased about 2 - fold the pretreatment values , and this increase declined gradually after dexamethasone withdrawal , reaching baseline values after 4 days . - pubmed
Furthermore , in groups exposed simultaneously to both drugs , dexamethasone completely prevented clenbuterol - induced decrease in lymphocyte beta2 - adrenergic receptor density and responsiveness . - pubmed
METHODS : Polytetrafluoroethylene ( PTFE ) grafts pretreated with fibronectin were seeded with SMC followed by ECs one day later and exposure to an in vitro flow system . - pubmed
Several studies have attempted to use biomarkers of biologically effective dose ( BBED ) such as polycyclic aromatic hydrocarbon - DNA and alfatoxin - albumin adducts to assess possible GxE interactions . - pubmed
[ Effectiveness of post - tonsillectomy aerosol penicillin therapy ] . - pubmed
Although it has been demonstrated exhaustively that potent and structurally diverse ATP - antagonistic small molecule kinase inhibitors can be found through mass screening and structure - guided design , the question of biochemical , cellular , and in vivo selectivity of such inhibitors remains much less clear . - pubmed
The adenosine A ( 2A ) receptor antagonist , 1,3,7 - trimethyl-8 -( 3 - chlorostyryl ) xanthine ( 0.5 or 1.0 mg / kg , i.p . ) - pubmed
, significantly reversed the pharmacological action of PAP 9704 in a dose related manner , but the adenosine A ( 1 ) receptor antagonist 8 - cyclopentyl-1,3 - dimethylxanthine ( 0.5 or 1.0 mg / kg , i.p . ) - pubmed
and the A ( 2B ) receptor antagonist alloxazine ( 1.5 or 3.0 mg / kg , i.p . ) - pubmed
Our results suggest that PAP 9704 prevents MA - induced hyperlocomotion , at least in part , via the stimulation of the adenosine A ( 2A ) receptor . - pubmed
Because mutations in both receptor genes appear to affect some parameters of cardiac function and because cardiac functional activity and energy metabolism are linked , we measured heart glucose utilization ( HMR(Glc ) ) in both the TRbeta(PV / PV ) and TRalpha(PV/+ ) mutants . - pubmed
6 -( 2 - Furanyl )- 9H - purin-2 - amine derivatives as A2A adenosine antagonists . - pubmed
Structure - activity relationships have been investigated through substitutions at the 9 - position of the 2 - amino-6 -( 2 - furanyl ) purine ( 5 ) to identify novel and selective A ( 2A ) adenosine receptor antagonists . - pubmed
Neither ovariectomy nor chronic treatment with melatonin affected plasma concentrations of leptin or the circadian pattern of secretion . - pubmed
Comparing different inducers , the best protection was achieved with BABA followed by jasmonic acid ( JA ) , whereas benzo ( 1,2,3 )- thiadiazole-7 - carbothionic acid - S - methyl ester ( a salicylic acid [ SA ] analog ) and abscisic acid ( ABA ) treatment did not increase the resistance significantly . - pubmed
Marker genes for the SA and JA pathways showed potentiated expression patterns in BABA - treated plants following infection . - pubmed
Application of the phenylalanine ammonia lyase inhibitor 2 - aminoindan-2 - phosphonic acid and the lipoxygenase ( LOX ) inhibitor 5 , 8 , 11 , 14 - eicosatetraynoic acid ( ETYA ) also led to a reduction of BABA - induced resistance ( BABA - IR ) , suggesting that callose deposition as well as defense mechanisms depending on phenylpropanoids and the JA pathways all contribute to BABA - IR . - pubmed
The results strongly suggest that the initial oxidation of PVA is mediated via a PVA oxidase , and not a PQQ - dependent dehydrogenase . - pubmed
Clinical outcomes after multivessel versus single - vessel percutaneous coronary intervention ( PCI ) in the era of stents , glycoprotein IIb / IIIa inhibitors , and clopidogrel pretreatment have not been well studied . - pubmed
Prolactin production in these extrapituitary sites is not governed by dopamine agonists , a finding that has spurred the production of prolactin receptor antagonists . - pubmed
The kappa - opioid receptor agonist nalfurafine suppressed herpetic and postherpetic pain to similar degrees . - pubmed
These results suggest that specific down - regulation of the mu - opioid receptor in the primary sensory neurons is responsible for the reduced analgesic action of morphine on postherpetic pain . - pubmed
Apoptotic morphology was completely prevented by the non - selective caspase inhibitor z - VAD - fmk , indicating an important role for caspases in neurodegeneration induced by street heroin . - pubmed
Nonetheless , in street heroin - treated cortical neurons , cytochrome c was released , accompanied by a decrease in mitochondrial potential and Bcl-2 / Bax . - pubmed
Pure heroin hydrochloride similarly decreased metabolic viability but only slightly activated caspase 3 . - pubmed
The phosphorylation of Akt occurred downstream of PI3 K and phospho - Akt was found to accumulate in the nucleus of IGF-1 - treated cells . - pubmed
Tetrahydrobiopterin shows chaperone activity for tyrosine hydroxylase . - pubmed
Tyrosine hydroxylase ( TH ) is the rate - limiting enzyme in the synthesis of catecholamine neurotransmitters . - pubmed
Primary inherited defects in TH have been associated with l - DOPA responsive and non - responsive dystonia and infantile parkinsonism . - pubmed
In this study , we show that both the cofactor ( 6R )- l - erythro-5,6,7,8 - tetrahydrobiopterin ( BH ( 4 ) ) and the feedback inhibitor and catecholamine product dopamine increase the kinetic stability of human TH isoform 1 in vitro . - pubmed
Our results also establish that orally administered BH ( 4 ) crosses the blood - brain barrier and therapeutic regimes based on BH ( 4 ) supplementation should thus consider the effect on TH . - pubmed
OBJECT : Transvenous embolization ( TVE ) for the treatment of a cavernous sinus ( CS ) dural arteriovenous fistula ( DAVF ) occasionally causes cranial nerve palsy ( CNP ) . - pubmed
INTRODUCTION : The action of cortistatin ( CST ) , a novel cyclic neuropeptide , as an anti - inflammatory factor has been studied , but few investigations have explored the immunomodulatory role of CST in transplantation . - pubmed
SMS 201 - 995 at the same concentrations did not have a significant effect on allograft survival ( MST , 8 days for both groups ) . - pubmed
AIM : This was to carry out a review of the literature concerning mineral trioxide aggregate ( MTA ) and Portland cement with regards to clinical , biological and mechanical findings and a possible substitution of MTA through Portland cement for endodontic use . - pubmed
Acyl - HSLs are synthesized by the LuxI family of synthases , and the signal response is mediated by members of the LuxR family of transcriptional regulators . - pubmed
thailandensis quorum - sensing mutants , and we used these mutants to show that BtaI1 is responsible for C(8)-HSL production and BtaI3 is responsible for 3OHC ( 8 )- HSL production . - pubmed
Importantly , we show that the BaffrDelta168 - 175 deletion mutant lacking the retrotransposon - displaced residues , and a BaffrT170A mutant lacking a critical threonine , supported B cell survival but failed to support optimal CD21 / 35 expression . - pubmed
Namely , pressurized liquid extraction ( PLE ) , using water and ethanol as solvents , supercritical fluid extraction ( SFE ) , using neat CO ( 2 ) and supercritical CO ( 2 ) modified with ethanol , as well as a novel extraction process called Water Extraction and Particle formation On - line ( WEPO ) are directly compared . - pubmed
Glyoxalase I [ lactoylglutathione lyase ( EC 4.4.1.5 ) encoded by GLO1 ] is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal , a cytotoxic byproduct of glycolysis . - pubmed
Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal - dependent protein adduction and cellular damage associated with diabetes , cancer , and chronological aging . - pubmed
Using two - dimensional proteomics followed by mass spectrometry the methylglyoxal - adducted protein was identified as heat shock protein 27 ( Hsp27 ; HSPB1 ) . - pubmed
Taken together , our data suggest a function of GLO1 in the regulation of detoxification and target adduction by the glycolytic byproduct methylglyoxal in malignant melanoma . - pubmed
In this paper , a chitosan - methotrexate covalently conjugated nanoparticles ( CS - MTX - TPP NPs ) has been developed as a potential delivery system for methotrexate ( MTX ) . - pubmed
MTX was first conjugated to CS by using glutaraldehyde as cross - linked agent , and followed by the process of ionic gelation between MTX - conjugated CS and sodium tripolyphosphate ( TPP ) to form CS - MTX - TPP NPs at mild reaction conditions . - pubmed
The hydrodynamic diameter of CS - MTX - TPP NPs and the encapsulation efficiency of MTX were affected by the weight ratio of MTX / CS . - pubmed
At the ratio of 1/11 , the mean hydrodynamic diameter of CS - MTX - TPP NPs was the lowest ( 187.9 + /- 9.0 nm ) and the nanoparticles presented the encapsulation efficiency of MTX with 53.0 + /- 3.2 % . - pubmed
The characterizations by atomic force microscopy ( AFM ) and photon correlation spectroscopy ( PCS ) showed the CS - MTX - TPP NPs had a spherical shape and good dispersion with diameter of sub-200 - nm and zeta potential of 30 mV. - pubmed
Additionally , in vitro release test revealed that the stable covalent bonding of CS and MTX was beneficial for providing slow release for MTX . - pubmed
Extremely accurate X - ray data were obtained for the explosive RDX ( hexahydro-1,3,5 - trinitro-1,3,5 - triazine ) at three different temperatures ( 20 , 120 and 298 K ) . - pubmed
For 1,3,5,7 - tetranitro-1,3,5,7 - tetraazacyclooctane ( HMX ) , old data collected at 20 and 120 K were re - integrated with the new version of VIIPP and refined in the same manner as for RDX . - pubmed
Limiting the refinement to a harmonic model of the atomic displacements may result in a biased and erroneous electron density , especially when atomic vibrations are significant ( as in RDX ) and at temperatures higher than obtained by using liquid helium . - pubmed
For RDX it was observed that the oxygen lone - pair concentrations of electrons are located close to perpendicular to the N - O bond vectors , which is typical for explosive materials . - pubmed
Blocks from 27 necropsy cases of prostatic carcinoma were sectioned and stained with H+E , fifteen biotinylated lectins chosen to probe for a wide range of oligosaccharide sequences within several categories of glycoprotein glycans , using a lectin - biotin avidin - peroxidase method , and monoclonal antibodies against Lewisa , sialyl Lewisa and sialyl Lewisx antigens . - pubmed
For discovery and replication we used more than 3,300 independent individuals from KORA F3 and F4 with metabolite measurements of 131 metabolites , including amino acids , phosphatidylcholines , sphingomyelins , acylcarnitines , and C6 - sugars . - pubmed
Sex - specific genome - wide association studies ( GWAS ) showed genome - wide significant differences in beta - estimates for SNPs in the CPS1 locus ( carbamoyl - phosphate synthase 1 , significance level : p<3.8x10(-10 ) ; Bonferroni - corrected threshold ) for glycine . - pubmed
Cytoskeletal changes and phosphorylation events mediated by Fyn activity were reversed by TM4SF10 overexpression , including a decrease in the activating tyrosine phosphorylation of Fyn ( Y(421 ) ) , suggesting TM4SF10 may have a regulatory role in suppressing Fyn activity . - pubmed
In addition , TM4SF10 was reexpressed following podocyte injury by puromycin aminonucleoside treatment , and its expression enhanced the abundance of high - molecular - weight forms of nephrin indicating it may participate in a mechanism controlling nephrin ' s appearance at the plasma membrane . - pubmed
The most potent PDE5 inhibitor was 5,7 - dimethoxyflavone ( IC(50 ) = 10.64 + - 2.09 muM , selectivity on PDE5 over PDE6 = 3.71 ) . - pubmed
Structure activity relationship showed that the methoxyl group at C-5 position of 7 - methoxyflavones was necessary for PDE5 inhibition . - pubmed
Moreover , 5,7 - dimethoxyflavones should make a useful lead compound to further develop clinically efficacious PDE5 inhibitors . - pubmed
In this work , a novel TSMP system comprised of both permanent covalent cross - links and supramolecular hydrogen bonding cross - links has been synthesized via a one - pot method . - pubmed
The in vivo role of nuclear receptor corepressors in thyroid hormone action . - pubmed
No significant decreases in the levels of GSH or total antioxidation capacity were found in the SR X - ray - irradiated testes . - pubmed
However , the SR X - ray at 40 Gy induced a marked increase in phosphorylated H2AX - a marker of double - strand DNA damage , which was significantly decreased by the antioxidant N - acetyl cysteine ( NAC ) . - pubmed
NAC also attenuated the SR X - ray - induced decreases in the cell layer number of seminiferous tubules . - pubmed
A skincare containing retinol adenosine and hyaluronic acid optimises the benefits from a type A botulinum toxin injection . - pubmed
SPA showed a positive correlation with high - sensitive C - reactive protein and a negative correlation with high - density lipoprotein cholesterol levels ( r = 0.313 , p = 0.021 and r = -0.233 , p = 0.036 , respectively ) . - pubmed
A combination of conventional TEM and PCR imaging allows one to detect complementary transmission and topographical information with nanometer precision of the same area of carbon - based samples . - pubmed
Functional gene categorization showed that genes associated with salicylic acid ( SA)-associated defenses , signal transduction , transport , and the secretory machinery are overrepresented in the group of SAR(+ ) genes , and that the group of SAR(- ) genes is enriched in genes activated via the jasmonate ( JA)/ethylene ( ET)-defense pathway , as well as in genes associated with cell wall remodeling and biosynthesis of constitutively produced secondary metabolites . - pubmed
Cluster II genes can be expressed independently of SA , and this group is moderately enriched in H2O2- and abscisic acid ( ABA)-responsive genes . - pubmed
In WHR , relaxin stimulated flow - mediated vasodilation ( 2.67 fold , from 48 + - 9 to 18 + - 4 mul / min , p = 0.001 ) , inhibited myogenic reactivity ( from -1 + - 2 to 7 + - 3 mum/10 mmHg , p = 0.01 ) , and decreased sensitivity to phenylephrine ( 28 % , from 1.39 + - 0.08 to 1.78 + - 0.10 muM , p<0.01 ) , but left compliance and RPFF unchanged . - pubmed
NO - blockade by L - NAME diminished most relaxin - mediated responses . - pubmed
In SHR , the vasodilator effects of relaxin were blunted for myogenic reactivity and sensitivity to phenylephrine , with similar effects on flow - mediated vasodilation , compliance , RPFF and equal Rxfp1 ( relaxin family peptide receptor ) gene expression , as compared to WHR . - pubmed
Three independent glc1 mutations were shown to have the same pleiotropic phenotype : catalase T deficiency , defective glycogen synthesis and defective trehalose accumulation . - pubmed
Following 1 - week rosiglitazone ( Rosi ) therapy , urinary PGE2 , but not other prostanoids , was reduced by 57 % in parallel with significant reduction of mPGES-1 protein and EP4 mRNA expressions . - pubmed
In line with the reduction of glomerular mPGES-1 , the glomerular injury score showed a tendency of improvement after 1 week of Rosi therapy . - pubmed
Collectively , the present study demonstrated an inhibitory effect of PPAR gamma activation on renal mPGES-1 / PGE2 / EP4 pathway in type-2 diabetes and suggested that mPGES-1 may potentially serve as a therapeutic target for treating type-2 diabetes - associated DN . - pubmed
Control of NF - kB activity in human melanoma by bromodomain and extra - terminal protein inhibitor I - BET151 . - pubmed
We show that I - BET151 is a potent , selective inhibitor of a number of NF - kB target genes involved in induction of inflammation and cell cycle regulation and downregulates production of cytokines such as IL-6 and IL-8 . - pubmed
A2B adenosine receptor stimulation decreased phosphorylation of extracellular signal - regulated kinases 1 and 2 ( ERK1 / 2 ) and stress - activated protein kinase / Jun - amino - terminal kinase ( SAPK / JNK ) at all three O2 concentrations . - pubmed
Adenosine receptor A2B stimulation decreased VEGF expression at 2 % and 8 % O2 but led to increased levels at 21 % O2 . - pubmed
RESULTS : After adjusting for age , sex , ethnicity , vitamin D , waist circumference , family poverty income ratio , total cholesterol , ever asthma , total protein , and survey weighting , abnormal gamma glutamyl transpeptidase was significantly associated with food sensitisation ( peanut : OR 2.17 , 95%CI 1.60 - 2.94 , P<0.001 ; egg : OR 2.55 , 95%CI 1.32 - 4.90 , P=0.008 ; milk : OR 2.59 , 95%CI 1.56 - 4.31 , P=0.001 ; shrimp : OR 1.81 , 95%CI 1.29 - 2.55 , P=0.002 ) . - pubmed
HG activates MMP-9 production , MMP-9 activity and MUC5AC overproduction , which is inhibited by nPG , DMSO and DPI ( inhibitors of ROS and NADPH ) , suggesting that HG - activated mucin synthesis is mediated by NADPH / ROS in 16HBE cells . - pubmed
These observations demonstrate an important role for MMP-9 activated by NADPH / ROS signaling pathways in regulating HG - induced MUC5AC expression . - pubmed
Epigallocatechin-3 - gallate enhances clearance of phosphorylated tau in primary neurons . - pubmed
EGCG is a potent antioxidant and an activator of the Nrf2 transcriptional pathway . - pubmed
Nrf2 activators including EGCG have shown promise in mitigating amyloid pathology in vitro and in vivo . - pubmed
RESULTS : EGCG treatment led to a significant decrease in the protein levels of three AD - relevant phospho - tau epitopes . - pubmed
Unexpectedly , EGCG does not appear to be facilitating this effect through the Nrf2 pathway or by increasing autophagy in general . - pubmed
However , EGCG did significantly increase mRNA expression of the key autophagy adaptor proteins NDP52 and p62 . - pubmed
DISCUSSION : In this study , we show that EGCG enhances the clearance of AD - relevant phosphorylated tau species in primary neurons . - pubmed
Taken together , these results demonstrate that EGCG has the ability to increase the clearance of phosphorylated tau species in a highly specific manner , likely through increasing adaptor protein expression . - pubmed
BACKGROUND : Certain proton pump inhibitors ( PPIs ) interfere with clopidogrel metabolism , potentially attenuating P2Y12 receptor inhibition . - pubmed
METHODS AND RESULTS : On - treatment P2Y12 platelet reactivity testing was performed using the VerifyNow assay after clopidogrel loading and successful drug - eluting stent implantation at 11 sites in the United States and Germany . - pubmed
In particular , human haemochromatosis protein ( HFE ) is involved in iron metabolism , and HFE H63D polymorphism has been related to the risk of amyotrophic lateral sclerosis and Alzheimer ' s disease . - pubmed
The bronchospasm related with remifentanil , post - ECT bradycardia , hypertensive crisis and oligohydramnios were also reported in one case each . - pubmed
Peroxidase ( POD ) catalyzed the polymerization of naturally occurring phenolics to give a variety of bioactive products . - pubmed
The guaiacol readily reacted in the POD system and afforded potential anti - microbial compounds . - pubmed
Secondary outcomes included the correlation of SctO2 with other relevant physiological variables , the dependence of SctO2 on baseline hemoglobin and transfusion , and the effect of red blood cell transfusion on fractional tissue oxygen extraction . - pubmed
Medical therapies including anticoagulation , statin therapy , and angiotensin - renin - aldosterone axis antagonists may reduce dementia risk . - pubmed
SL Dams and MSG Dams presented an increase in WAT content and higher plasma levels of glucose , triglycerides , and insulin , in relation to NL Dams and CON Dams , respectively . - pubmed
Therefore , we evaluated HD cell responses to visual cue rotations in a familiar arena , and during navigation between familiar and novel arenas , after muscarinic receptor blockade with systemic atropine . - pubmed
Protein arginine methyltransferase 1 ( PRMT1 ) catalyses the methylation of substrate arginine by transferring the methyl group from SAM ( S - adenosyl - l - methionine ) , which leads to the formation of S - adenosyl homocysteine ( SAH ) and methylated arginine . - pubmed
We have shown previously that the Asp84 on PRMT1 could be a potential inhibitor binding site . - pubmed
Our results show for the first time that PRMT1 inhibitors can target the Asp84 binding site , which will be helpful for future drug discovery studies . - pubmed
Levels of serum creatinine ( Cr ) in each EGCG - treated group were decreased but not significant ; the activities of hepatic xanthine oxidase ( XOD ) and adenosine deaminase ( ADA ) in high dose groups ' EGCG were notably lower than those of model group . - pubmed
Ultrasound-(US ) propelled nanowires consisting of Au / Ni / Au / PEDOT - PPy - COOH segments are modified with asparaginase enzyme and applied as an effective anti - cancer agent . - pubmed
The fast motion of the motor - carrying asparaginase leads to greatly accelerated biocatalytic depletion of asparagine and hence to a significantly enhanced inhibition efficacy against El4 lymphoma cancer cells ( 92 % ) as compared to free enzyme counterpart ( 17 % ) and other control groups . - pubmed
BACKGROUND : Recent guidelines recommend riociguat , a soluble guanylate cyclase ( sGC ) stimulator , and the type 5 phosphodiesterase inhibitor ( PDE5i ) tadalafil or sildenafil as treatments for pulmonary arterial hypertension . - pubmed
These conjugates have different DNA surface densities and thus exhibit different levels of nuclease resistance , cellular uptake , and gene regulation capabilities ; the properties displayed by the C60 SNA conjugate are closer to those of conventional and prototypical gold nanoparticle SNAs . - pubmed
Proline substitutions in a Mip - like peptidyl - prolyl cis - trans isomerase severely affect its structure , stability , shape and activity . - pubmed
Unlike rFKBP22 , the three mutants were unfolded by a non - two state mechanism in the presence of urea . - pubmed
Conversely , the rapamycin binding affinity of no mutant was different from that of rFKBP22 . - pubmed
The inhibitor [ BDA - Fe ( III ) ] was designed to possess two functional groups : the anthraquinone moiety , as recognition element by hGSTP1 - 1 and a metal chelated complex [ iminodiacetic acid - Fe ( III ) ] as a reactive moiety , able to generate reactive oxygen species ( ROS ) , through Fenton reaction . - pubmed
Upon binding of the BDA - Fe ( III ) to hGSTP1 - 1 in the presence of hydrogen peroxide , reactive oxygen species ( ROS ) are generated , which promoted the specific cleavage of hGSTP1 - 1 in a time and concentration - dependent manner . - pubmed
In this study , the effects of two weeks of daily subcutaneous exposure to the OP - nerve agent diisopropylfluorophosphate ( DFP ) in doses ranging from 0.125 - 0.500 mg / kg on whole brain volume , white matter , and gray matter integrity were evaluated in post mortem tissues using histology and magnetic resonance imaging ( MRI ) methods . - pubmed
DFP was associated with dose - dependent impairments in red blood cell and brain AChE ( down to 29 and 18 % of control , respectively at the highest dose ) , 24 h after the last injection . - pubmed
Moreover , histological and MRI analysis of post mortem brains did not reveal any pronounced alterations of whole brain , white matter , or gray matter volumes associated with DFP . - pubmed
OBJECTIVES : The most challenging stage of cardiac resynchronization therapy ( CRT ) is coronary sinus cannulation ( CS ) . - pubmed
METHODS : Twelve well - trained , male triathletes ( aged 21 - 47 yr ) were assigned in a randomized , double - blind , crossover design to receive 70 ml of BJ ( 6.5 mmol NO3- ) or placebo ( PL ) . - pubmed
For 40 dpf ( days post - fertilisation ) F0 generations , estradiol hormone and vitellogenin ( Vtg ) contents , the expression levels of Vtg1 , P450 aromatase ( Cyp19a and Cyp19b ) , 17beta - hydroxysteroid dehydrogenase ( Hsd17b1 , Hsd17b3 ) , estrogen receptor - alpha and progesterone receptor in all concentration groups decreased significantly , while androgen receptor increased significantly in 10 and 100 mug / L exposure groups compared with the corresponding control group , showing anti - estrogen and androgen effects . - pubmed
For 120 dpf F0 generations , acetylcholinesterase activity was significantly decreased and glutathione and malondialdehyde levels , superoxide dismutase , catalase and glutathione reductase activities were significantly increased in all treatment groups compared with the corresponding control group . - pubmed
Data on lifetime history of alcohol use , DSM - IV use disorders , and remission were collected from 1,459 adults using face - to - face interviewers based on the Composite International Diagnostic Interview ( CIDI 3.0 ) . - pubmed
By using one of the sulfate oxygen atoms as a third donor in addition to the bidentate pyridinone moiety , BAL30072 can form a L2 M complex , which was predicted to be the one with the best binding affinity to PiuA. - pubmed
Vitamin D and probiotic co - supplementation was associated with a significant reduction in total testosterone ( beta - 0.19 ng / mL ; 95 % CI , - 0.28 , - 0.10 ; P & lt ; 0.001 ) , hirsutism ( beta - 0.95 ; 95 % CI , - 1.39 , - 0.51 ; P & lt ; 0.001 ) , high - sensitivity C - reactive protein ( hs - CRP ) ( beta - 0.67 mg / L ; 95 % CI , - 0.97 , - 0.38 ; P & lt ; 0.001 ) and malondialdehyde ( MDA ) levels ( beta - 0.25 mumol / L ; 95 % CI , - 0.40 , - 0.10 ; P = 0.001 ) , and a significant increase in total antioxidant capacity ( TAC ) ( beta 82.81 mmol / L ; 95 % CI , 42.86 , 122.75 ; P & lt ; 0.001 ) and total glutathione ( GSH ) levels ( beta 40.42 mumol / L ; 95 % CI , 4.69 , 76.19 ; P = 0.02 ) , compared with the placebo . - pubmed
A total of 868 FET cycles were included and randomized into two groups : Group A ( n = 434 ) received GnRHa 0.1 mg subcutaneous injection on day 3 after embryo transfer ( ET ) ; Group B ( n = 434 ) did not receive GnRHa . - pubmed
In conclusion , single dose of GnRHa ( 0.1 mg triptorelin acetate ) supplementation 3 days after ET in AC - FET cycles did not show significant benefit on pregnancy outcomes as a whole . - pubmed
Consequently , the selected antigenic amino acids sequences ( B - cell epitopic regions ) were analyzed for T - cell epitopes determination ( MHC I and MHC II alleles binding sequence ) performing the ProPred 1 and ProPred server respectively . - pubmed
albicans , whilst a low voriconazole concentration ( 0.25 microg / mL ) blocked their interactions similar to the effect of the erg11Delta / Delta mutant . - pubmed
Instead , the intron - retained enzyme BGL1B had a better thermostability with a higher optimal temperature and a longer half - life under 50 C. Also it exhibited a little higher kcat for 4 - nitrophenyl - beta - d - glucopyranoside ( PNPG ) and a noticeable higher hydrolysis efficiency towards geniposide . - pubmed
Other serum parameters including alanine aminotransferase , aspartate aminotransferase , alkaline phosphatase , total bilirubin , blood urea nitrogen and interleukin-6 were significantly higher in the overgrazing group . - pubmed
Two - year CW - free survival was 81 % and 58 % , respectively ( HR CW macitentan 2.16 [ 0.962 - 4.87 ] , p = 0.06 ) . - pubmed
RAC - alpha serine / threonine - protein kinase ( AKT1 ) , caspase-3 ( CASP3 ) , caspase-8 ( CASP8 ) , mitogen - activated protein kinase 8 ( MAPK8 ) , and mitogen - activated protein kinase 14 ( MAPK14 ) are the core targets regulated by stemazole and play a central role in its anti - apoptosis effects . - pubmed
AIM AND OBJECTIVE : In this work , water was used as solvent for the eco - friendly synthesis of imines under microwave irradiation . - pubmed
Active vitamin D and vitamin D analogs stimulate fibroblast growth factor 23 production in osteocyte - like cells via the vitamin D receptor . - pubmed
Osteocytes play an important role in the regulation of serum phosphorus by producing fibroblast growth factor 23 ( FGF23 ) . - pubmed
FGF23 production is stimulated by 1alpha,25 - dihydroxyvitamin D in osteocytes . - pubmed
However , it is unclear whether vitamin D induces FGF23 production in osteocytes directly . - pubmed
We also investigated differences in the induction of FGF23 by 1alpha,25 - dihydroxyvitamin D and various vitamin D analogs . - pubmed
MCT3 - E1 - OLCs were stimulated with 1alpha,25 - dihydroxyvitamin D3 and subsequent FGF23 gene expression was 2631 + - 605 times higher compared with untreated cells . - pubmed
Therefore , the induction of FGF23 in osteocytes by vitamin D may be primarily mediated via VDR . - pubmed
The potential of 25 ( OH ) vitamin D3 , paricalcitol , and maxacalcitol to induce FGF23 production was almost the same as that of 1alpha,25 - dihydroxyvitamin D3 . - pubmed
However , falecalcitriol and eldecalcitol demonstrated a reduced potential to induce FGF23 compared with 1alpha,25 - dihydroxyvitamin D3 . - pubmed
Our results demonstrate that FGF23 induction is different among the analogs of 1alpha,25 - dihydroxyvitamin D3 . - pubmed
Self - focusing Au@SiO2 nanorods with rhodamine 6 G as highly sensitive SERS substrate for carcinoembryonic antigen detection . - pubmed
A highly sensitive self - focusing surface - enhanced Raman scattering ( SERS ) methodology has been developed using Au@SiO2 core - shell nanorods for carcinoembryonic antigen ( CEA ) detection . - pubmed
The SERS enhancement factor was evaluated for anisotropic Au@SiO2 nanorods with silica shells of various thicknesses , upon which Rhodamine 6 G ( R6 G ) dye was applied as a reporter molecule for the quantitative determination of CEA . - pubmed
METHODS : In a large cohort study of the German Neonatal Network ( GNN ; n = 16 967 VLBWI ) we assessed the association of vancomycin treatment and pathological hearing tests at discharge and at 5 year follow - up . - pubmed
RESULTS : In the GNN cohort , 28 % ( n = 4739 ) were exposed to IV vancomycin therapy . - pubmed
Reactive oxygen species produced from PVAT can be originated from mitochondria , nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidases , and uncoupled endothelial nitric oxide synthase . - pubmed
Association Between Hypertriglyceridemic Waist Phenotype and Increased Urinary Albumin - Creatinine Ratio in Chinese Adults : The REACTION Study . - pubmed
Additionally , strong evidence indicates that elevated urinary albumin - creatinine ratio ( UACR ) is associated with increased prevalence of CVD . - pubmed
Furthermore , the stratified analysis showed that the OR for high - risk significantly increased in individuals in the HTGW group aged below 65 years , with 5.6 < = fasting blood glucose < 7.0 or 7.8 < = post - load blood glucose < 11.1 mmol / L , 120 < = systolic blood pressure < 140 or 80 < = diastolic blood pressure < 90 , 24 < = body mass index < 28 kg / m2 , and estimated globular filtration rate > 90 mL / min per 1.73 m2 . - pubmed
FGF significantly inhibited Abeta fibrillogenesis and disintegrated the mature fibrils as evidenced by thioflavin T fluorescence and atomic force microscopy studies . - pubmed
Molecular dynamics simulations further showed that FGF could synergistically interact with the Abeta17 - 42 pentamer via electrostatic interactions , hydrogen bonds and pi - pi interactions , which reduced the beta - sheet content , and disordered random coils and bend structures of the Abeta17 - 42 pentamer . - pubmed
Functional enrichment analysis found several biological processes , including protein targeting to membrane , ribosome and negative regulation of programmed cell death , were over - represented exclusively among up - regulated DEGs in the resistant reaction , whereas significant enrichment of salicylic acid ( SA ) and jasmonic acid ( JA ) pathways observed for down - regulated DEGs occurred only in the susceptible reaction . - pubmed
In addition , a functionally - related calcium channel , the transient receptor potential vanilloid type 4 ( TRPV4 ) channel , has recently been linked to purinergic signaling and has also been shown to mediate lung IRI . - pubmed
The nine analytes selected for the program were amylase , calcium , phosphorus , sodium , potassium , creatinine , osmolality , 17 - ketosteroids and total protein . - pubmed
Data obtained from more than 230 enrolled laboratories indicate deficiencies in assay performance for amylase , 17 - ketosteroids and total protein as measured by consensus evaluation . - pubmed
Of these isolates 12.6 % produced beta - lactamase and were resistant to ampicillin . - pubmed
Haloperidol reduced the flash fusion threshold frequency at 6 h post - dose . - pubmed
Sulpiride prolonged the duration of the manipulative motor task , particularly at 48 h post - dose . - pubmed
Chlorpromazine significantly reduced the number of correct letter pair identifications at 2 , 4 and 6 h post - dose , haloperidol at 4 , 6 , 8 , 24 and 48 h post - dose , and sulpiride at 24 h post - dose. - pubmed
The in vivo and in vitro oxidation of molecular form 1 of biliverdin reductase to molecular form 3 by diamide . - pubmed
Although hepatic levels of GSH also decreased , no mixed disulfides of the reductase and GSH could be detected . - pubmed
The diamide also oxidized molecular form 1 of biliverdin reductase in vitro and molecular form 3 was formed . - pubmed
A competitive assay in which the labelled antigen was irradiated at 254 nm was found to be specific for Pyr ( 6 - 4 ) Pyo adducts , the antibody - binding sites being sensitive to a secondary photolytic dose of 320 - nm light . - pubmed
On the horizon are rimantadine and the interferons . - pubmed
When correlated with the known structure of the HLA - B27 subtypes , these results demonstrate the critical relevance of amino acid changes within residues 77 - 81 and at position 152 in modulating allospecific CTL recognition of HLA - B27.1 and suggest that these residues could be involved in the structure of immunodominant regions of this antigen . - pubmed
The observed cross - reactions with HLA - B40 , differing from B27.1 in 16 amino acid residues , suggest that the simultaneous occurrence of multiple amino acid changes could have mutually compensatory effects , so that a cross - reactive epitope might result from various combinations of polymorphic residues . - pubmed
Stimulation of postsynaptic D2 - dopamine receptors by B - HT 958 is revealed by co - treatment with the D1 - receptor agonist SKF 38393 . - pubmed
The motor activity was only slightly stimulated by B - HT 958 or by the D1 - receptor agonist SKF 38393 but it was markedly increased by the two drugs given in combination . - pubmed
The enhanced motor activity was inhibited by the D2 - receptor antagonist sulpiride or the D1 - receptor antagonist SCH 23390 , indicating that it was caused by stimulation of both receptor types . - pubmed
Isolated light chain of tetanus toxin inhibits exocytosis : studies in digitonin - permeabilized cells . - pubmed
It has been concluded that the paradox response of the muscle membrane to changes in [ K+]o can be attributed to the K - dependent conductance changes of the inward rectifier K channel providing an explanation for the plateau - formation of SMPO and for the existence of two stable levels of membrane potential of the skeletal muscle bathed in Cl- -free medium . - pubmed
Millisecond kinetics of ATP synthesis driven by externally imposed electrochemical potentials in chloroplasts . - pubmed
We have used rapid mixing and quenching techniques to measure the initial ATP synthesis rates and the duration of the ATP synthetic capacity derived from artificially imposed proton gradients and valinomycin - mediated K+ diffusion potentials in chloroplasts . - pubmed
The serum alkaline phosphatase was significantly more accurate than total bilirubin and SGOT in detecting liver metastasis . - pubmed
The sensitivity , specificity , and accuracy of the imaging studies in the presence of an abnormal alkaline phosphatase , SGOT , or total bilirubin were not significantly different than in the presence of normal biochemical tests . - pubmed
Blood - glucose estimations were performed on this sample by an automated glucose - oxidase method and by a reflectance meter system ( Reflocheck , BCL ( London ) Ltd. , Lewes , East Sussex , UK ) . - pubmed
Blood - glucose values measured by the meter were found to be higher than those assayed by the glucose - oxidase method by a mean ratio of 1.16:1 . - pubmed
We conclude that hypertrophic chondrocytes respond directly to ICF and CCF by an increased deposition of calcium - phosphate mineral in the matrix . - pubmed
Cirazoline produced a postsynaptic alpha 1 - adrenoceptor mediated potentiation of the isometric contraction to single pulse field stimulation in both prostatic and epididymal portions . - pubmed
In epididymal portions , nifedipine ( 10 microM ) greatly attenuated the postsynaptic alpha 1 - receptor mediated potentiation of nerve mediated contractions , uncovering a presynaptic inhibitory action of cirazoline . - pubmed
No evidence was found for alpha 2 - antagonism by cirazoline . - pubmed
Glucose uptake was similar in both groups being 65.4 + /- 24.1 % of control in the CS group and 73.9 + /- 29.8 % in the AM group . - pubmed
Fixation in formol acetic acid was unsatisfactory for demonstration of fibronectin ; prolonged fixation in formol sublimate was unsatisfactory for demonstration of laminin . - pubmed
Trypsin was completely ineffective for unmasking laminin antigens except after fixation in ethanol acetic acid ; it was only partially effective for showing fibronectin antigens . - pubmed
Synthetic peptide tyrosine phosphorylation was stimulated by epidermal growth factor but not by insulin or relaxin . - pubmed
The following components were tested : T protein , M protein , opacity factor , lipoteichoic acid , hyaluronic acid , streptolysin S , streptolysin O , DNase , hyaluronidase and NADase . - pubmed
They increased the amount of hyaluronic acid and hyaluronidase , decreased the amount of M protein , and enhanced phagocytosis and the release of lipoteichoic acid . - pubmed
In contrast , scopolamine impaired working memory in both MTS and NMTS tasks . - pubmed
Individual neomycin resistant colonies were screened for alkaline phosphatase ( AP)-specific staining . - pubmed
Both AP activity and osteocalcin ( OC ) secretion increased in a dose - dependent manner following dihydroxyvitamin D3 ( 1,25 - D3 ) treatment when cultured at either temperature . - pubmed
Serum samples of autochthonous individuals from the Garrotxa prepyrenean region , and the Pallars Sobira valley were screened for HP ( Haptoglobin ) and ORM ( Orosomucoid ) types by polyacrylamide isoelectric focusing and electrophoresis . - pubmed
For immunostaining , the avidin - biotin complex technique was performed on paraffin sections of cell blocks , using a standardized automatic immunostainer . - pubmed
Crystal structure at 1.9 - A resolution of human immunodeficiency virus ( HIV ) II protease complexed with L-735,524 , an orally bioavailable inhibitor of the HIV proteases . - pubmed
L-735,524 is a potent , orally bioavailable inhibitor of human immunodeficiency virus ( HIV ) protease currently in a Phase II clinical trial . - pubmed
Both Fourier and difference Fourier maps reveal clearly the closure of the flap domains of the protease upon L-735,524 binding . - pubmed
Specific interactions between the enzyme and the inhibitor include the hydroxy group of the hydroxyaminopentane amide moiety of L-735,524 ligating to the carboxyl groups of the essential Asp-25 and Asp-25 ' enzymic residues and the amide oxygens of the inhibitor hydrogen bonding to the backbone amide nitrogen of Ile-50 and Ile-50 ' via an intervening water molecule . - pubmed
Although other hydrogen bonds also add to binding , an equally significant contribution to affinity arises from hydrophobic interactions between the protease and the inhibitor throughout the pseudo - symmetric S1 / S1 ' , S2 / S2 ' , and S3 / S3 ' regions of the enzyme . - pubmed
The mean ( + /- standard deviation ) rates of penetration into the cerebrospinal fluid ( CSF ) of infected animals were 6.7 + /- 3.9 % for piperacillin given alone and 36.3 + /- 21.9 % for tazobactam given alone . - pubmed
The study shows that tazobactam is able to provide effective protection against piperacillin hydrolysis by the TEM-3 enzyme within the CSF . - pubmed
Appropriate dosage regimens of various beta - lactam - tazobactam combinations may deserve comparative studies in experimental meningitis caused by organisms producing extended - spectrum beta - lactamases . - pubmed
Biochemical analysis revealed normal values for carnitines , carnitine palmityl transferase in serum and striated muscle , and beta - oxidation enzymes in striated muscles . - pubmed
dose of benzodiazepine ( BZD ) receptor ligand methyl beta - carboline-3 - carboxylate ( beta - CCM ) , whether or not preceded by administration of two lower doses of beta - CCM ( 0.5 and 1 mg / kg i.p . ) - pubmed
In the other 4 strains , such injections induced either tolerance ( CPB - K , NZB ) , or sensitization ( C57BL/6J , XLII ) , whatever the compound subsequently administered ( beta - CCM or saline ) . - pubmed
When BMR is titrated with NADPH or sodium dithionite under anaerobic conditions , addition of 2 electron equivalents per mole of BMR results in the reduction of the high potential flavin ( FMN ) without the accumulation of semiquinone intermediates . - pubmed
Our results indicate that the kinetic and thermodynamic control of the flavins in BMR is significantly different from that in microsomal P450 reductase . - pubmed
The low potential of the anionic FMN semiquinone can be utilized to reduce the P450 heme . - pubmed
When the anionic semiquinone becomes protonated , its potential becomes more positive and it is readily reduced to FMNH2 , which is not capable of reducing P450 . - pubmed
In the activation of adrenergic neurotransmission by a ganglionic nicotinic agonist , elevation of plasma NPY - ir was also reduced by propofol indicating the direct effect on peripheral adrenergic nerve terminals . - pubmed
Plasma level of NPY - ir reversed in parallel with the recovery of anaesthesia induced by propofol . - pubmed
Thus , a central nervous system effect of propofol can also be considered in its effect on plasma NPY - ir . - pubmed
[ Combined therapy of malignant tumors of the upper jaw and nasal cavity using pre- and post - operative radiation with metronidazol radiomodification ] . - pubmed
These structures are of increasing interest since they may function as adhesion molecules ; adhesion of tumour cells to endothelial cells of blood vessels may be mediated by an interaction between sialosyl - Le ( x ) and E - selectin and studies indicate that Le ( y ) is related to cell motility . - pubmed
The TCA extract inhibited the activity of type 1 protein phosphatase by 20 + /- 4 % ( n = 3 expts ) . - pubmed
Dephosphorylation of the phosphorylated TCA extract by type 2 protein phosphatase reduced inhibition to 2 + /- 0.2 % ( n = 3 expts ) . - pubmed
To determine whether isoproterenol increases phosphorylation of PPI-1 in cardiomyocytes , the TCA extracts were prepared from cardiomyocytes treated with 1 microM isoproterenol and from untreated cardiomyocytes . - pubmed
With the use of a rabbit skeletal muscle PPI-1 antibody , immunoblots of the TCA extract of cardiomyocytes identified a 28 - kDa protein . - pubmed
Hepatic low density lipoprotein ( LDL ) receptor expression was not affected by pravastatin throughout the experimental period . - pubmed
Further analysis of G protein cytoplasmic domain deletion mutants showed that a cytoplasmic domain of only 1 amino acid did not drive efficient budding , whereas 9 amino acids did . - pubmed
Post - allo BMT bone loss correlated best with the cumulative prednisolone dose at the LS and FN , and with average daily prednisolone dose for TBBMC . - pubmed
When the rhG - CSF was secreted to extracellular broth in the presence of a non - ionic surfactant ( Tween 80 ) in the culture media , the multimerization of the secreted rhG - CSF was efficiently prevented in the fed - batch cultures . - pubmed
The catecholamine - mediated modulation of the cytokine network has primarily been demonstrated for leukocytes . - pubmed
Whereas catecholamines decrease the LPS - induced production of IL-6 by leukocytes , serum levels of IL-6 are dramatically increased by the catecholamine epinephrine in animal endotoxemia models . - pubmed
Furthermore , these catecholamines could even potentiate the LPS - induced IL-6 protein production . - pubmed
The catecholamine - induced IL-6 stimulation is based on increased IL-6 mRNA levels . - pubmed
Thus , endothelial cells might be a possible source of increased IL-6 production observed in situations such as stress or septic shock , in which catecholamines are elevated due to endogenous production or exogenous application . - pubmed
Use of multivitamin supplements containing folic acid diminished the adverse effects of dihydrofolate reductase inhibitors , but not that of antiepileptic drugs . - pubmed
The mechanisms involved in this selection process are not completely defined but appear to involve acquisition of LH receptors on granulosa cells of the dominant follicle , increased estradiol production by the dominant follicle , and inhibition of circulating FSH concentrations . - pubmed
At the time of selection of the dominant follicle , the normal increase in circulating estradiol concentrations and subsequent depression in circulating FSH is blunted due to estradiol metabolism . - pubmed
In the case of the immune response to the hapten ( 4 - hydroxy-3 - nitrophenyl ) acetyl ( NP ) , as with several other designed immunogens , the process is dominated by a single key mutation , which greatly simplifies the modeling of and analysis of data . - pubmed
CYP3A4 expression was assessed by Western blotting and by immunohistochemistry ; activity was measured by the rate of formation of 6beta - hydroxytestosterone from testosterone . - pubmed
Ondansetron : a selective 5 - HT ( 3 ) receptor antagonist and its applications in CNS - related disorders . - pubmed
Ondansetron is a selective 5 - hydroxytryptamine ( 3 ) ( 5 - HT ( 3 ) ) receptor antagonist that has been introduced to clinical practice as an antiemetic for cancer treatment - induced and anesthesia - related nausea and vomiting . - pubmed
OBJECTIVE : The aim of the present study was to investigate the relationship between the changes in lean and fat mass during gonadotropin - releasing hormone agonist ( GnRH agonist ) therapy . - pubmed
They were given GnRH agonist ( leuprorelin acetate , 3.75 mg ) monthly for 4 months . - pubmed
The present study analyzed using immunohistochemical labeling the distribution and co - localization of nitric oxide synthase ( NOS ) , cocaine- and amphetamine - regulated transcript peptide ( CARTp ) and pituitary adenylate cyclase activating polypeptide ( PACAP ) with choline acetyltransferase ( ChAT)-immunoreactive fibers in the guinea - pig stellate ganglia . - pubmed
As calcium and phosphorus increased in the aortic valve , the mass ratios Mg / Ca and Mg / P decreased reversely in the aortic valve . - pubmed
FS and PME pretreatments produced a dose - dependent protection against CCl 4 hepatotoxicity , with the effect of FS being more potent . - pubmed
The ensemble of results indicates that FS / PME produced a more potent in vivo antioxidant action than alpha - Toc by virtue of their ability to enhance hepatic mitochondrial glutathione antioxidant status and that the differential potency of FS and PME can be attributed to the difference in in vivo antioxidant potential between Sch B and emodin . - pubmed
The addition of tocopherol , ascorbate , prostaglandin E2 and lipoxygenase inhibitors delayed cell death , whereas the inhibition of cyclooxygenase promoted AA - induced cell death . - pubmed
The mechanism did not involve blockage of the interleukin-1 receptor , because interleukin-12 did not suppress interleukin-1 - mediated induction of collagenase mRNA . - pubmed
Here we describe a paralogue of EBA-175 and show that this protein ( EBA-181 / JESEBL ) binds in a sialic acid - dependent manner to erythrocytes . - pubmed
EM574 , a motilide , has an orexigenic activity with affinity for growth - hormone secretagogue receptor . - pubmed
The most commonly employed humanized and chimeric MoAbs are rituximab , alemtuzumab ( Campath-1H , Ilex Pharmaceuticals , San Antonio , TX ) , and gemtuzumab - ozogamicin ( Mylotarg , Wyeth - Ayerst Laboratories , St Davids , PA ) . - pubmed
Dihydrodipicolinate reductase ( DHPR ) catalyzes the reduced pyridine nucleotide - dependent reduction of the alpha , beta - unsaturated cyclic imine , dihydrodipicolinate , to generate tetrahydrodipicolinate . - pubmed
tuberculosis DHPR uses NADH and NADPH with nearly equal efficiency based on V / K values . - pubmed
Insulin sensitivity ( M - value ) was evaluated by the euglycaemic hyperinsulinaemic glucose clamp technique . - pubmed
The EHT - R had significantly higher levels of fasting insulin and triglyceride than did the NT and the EHT - N. The EHT - R had higher levels of free fatty acid and lower levels of high - density lipoprotein cholesterol than did the EHT - N. The M - value was negatively correlated with fasting insulin , free fatty acid , and triglyceride . - pubmed
Approaches that have proven to be effective in animal models include the use of multi - epitope vaccines incorporating highly variable amino terminal serotypic determinants , those based on the carboxy terminal conserved region and combination vaccines incorporating both serotypic and conserved region determinants of the M protein . - pubmed
Standard REV of phosphatidylcholine ( PC):cholesterol ( CHOL ) in 7:3 molar ratio , and photoactive REV of PC : CHOL : SPF , and DPPC : CHOL : SPF in 7:3:3 molar ratio were prepared . - pubmed
DPPC led to a higher liposomal membrane stability ( lower CAL release ) than PC . - pubmed
Dietary intake and serum calcium , magnesium , iron , retinol , alpha - tocopherol , albumin , cholesterol , glucose and hematological parameters were determined . - pubmed
Mean serum alpha - tocopherol / cholesterol ratio in the whole population was 6.3 + /- 2.3 mmol / mol without gender differences . - pubmed
Interaction of aldehydes with collagen : effect on thermal , enzymatic and conformational stability . - pubmed
formaldehyde , gluteraldehyde , glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal , enzymatic and conformational stability of collagen . - pubmed
The variation in the thermal stability of collagen brought about by aldehydes was in the order of formaldehyde & gt ; gluteraldehyde & gt ; glyoxal & gt ; crotanaldehdye . - pubmed
The aldehydes also impart a high degree of stability to collagen against the activity of the degrading enzyme , collagenase . - pubmed
The effect of various aldehydes on the secondary structure of collagen was studied using circular dichroism and it was found that the aldehydes lead to changes in the amplitude of the circular dichroic ( CD ) spectrum but did not alter the triple helical conformation of collagen . - pubmed
The secondary structure of collagen is not significantly altered on interaction with different aldehydes . - pubmed
BACKGROUND : The associations of proteinuria and an elevated creatinine level with progression to acquired immunodeficiency syndrome ( AIDS ) and death in the era of highly antiretroviral therapy ( HAART ) have not been fully described . - pubmed
We suggest the [ ( 99m ) Tc]OKT3 scans may be useful for the monitoring of renal transplants to detect acute rejection . - pubmed
These results show that mGluR4 may play a role in locomotor activity in general and also display specificity for mediation of the motor stimulant effect of ethanol . - pubmed
Activation of NF - kappaB signalling and TNFalpha - expression in THP-1 macrophages by TiAlV- and polyethylene - wear particles . - pubmed
In this study we evaluated the effects of polyethylene- and TiAlV - particles on activation of NF - kappaB signalling pathways and TNFalpha biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis . - pubmed
A significant TNFalpha release was detected after 360 min exposure to polyethylene- and TiAlV - particles in a dose dependent manner . - pubmed
Muscle cytokine mRNA changes after 2.5 h of cycling : influence of carbohydrate . - pubmed
Blood cell counts were determined , and plasma was analyzed for interleukin ( IL )- 6 , IL-10 , IL-1 receptor antagonist ( ra ) , IL-8 , cortisol , epinephrine , glucose , and insulin . - pubmed
RESULTS : Plasma glucose and insulin were higher , and epinephrine , cortisol , IL-6 , IL-10 , and IL-1ra , but not IL-8 , were significantly lower postexercise in CHO versus PLA . - pubmed
The presence of five TAMs in the 41 , 215 pathway , at codons 41 , 67 , 210 , 215 , and 219 of reverse transcriptase ( RT ) , produced a median 1.8 - fold reduction in SPD754 susceptibility , compared with fold reductions to zidovudine , lamivudine , abacavir , didanosine and tenofovir of 438 , 4.8 , 4.5 , 1.4 and 3.6 , respectively . - pubmed
This 2 - part , double - blind , placebo - controlled study was conducted to determine the safety and efficacy of etoricoxib , a COX-2 selective inhibitor , for the treatment of hemophilic arthropathy . - pubmed
Studies have provided evidence that HSL , though possessing triacylglycerol lipase activity , appears to be the rate - limiting enzyme for cholesteryl ester and diacylglycerol hydrolysis in adipose tissue and is essential for complete hormone stimulated lipolysis , but other triacylglycerol lipases are important in mediating triacylglycerol hydrolysis in lipolysis . - pubmed
HSL knockouts are resistant to both high fat diet - induced and genetic obesity , displaying reduced quantities of white with increased amounts of brown adipose tissue , increased numbers of adipose macrophages , and have multiple alterations in the expression of genes involved in adipose differentiation , including transcription factors , markers of adipocyte differentiation , and enzymes of fatty acid and triglyceride synthesis . - pubmed
Significant increases in mRNA expressions of some metabolism- and oxidative stress - related genes with a formation of gamma - glutamyltranspeptidase ( GGT ) positive foci were observed in the DMN + DC + PH group by the treatment of DC for 13 and 26 weeks . - pubmed
RESULTS : There were significant differences in the age , male , alcohol - consuming , over - salt intake , average systolic pressure , average diastolic pressure , waist - to - hip - ratio , total cholesterol , high - density lipoprotein , and low density lipoprotein between the hypertensive sib and the normotensive sib ( P & lt ; 0.05 ) . - pubmed
Furthermore , IFN - gamma pretreatment of NB cells increased doxorubicin - induced apoptosis nearly 3 - fold . - pubmed
Methylenetetrahydrofolate reductase ( MTHFR ) , a key regulatory enzyme in the metabolism of folate , is suspected to play a role in the etiology of cancer , via its effects on DNA methylation and nucleotide synthesis . - pubmed
One of 2 lines displayed a decrease in the number of osteoblasts , but in vivo bromodeoxyuridine labeling demonstrated that CHOP overexpression did not have an effect on osteoblastic cell replication . - pubmed
BACKGROUND , AIMS AND SCOPE : In a previous study , we explored the use of acetate , lactate , molasses , Hydrogen Release Compound ( HRC , which is based on a biodegradable poly - lactate ester ) , methanol and ethanol as carbon source and electron donor to promote bacterial sulfate reduction in batch experiments , this with regards to applying an in situ metal precipitation ( ISMP ) process as a remediation tool to treat heavy metal contaminated groundwater at the site of a nonferrous metal work company . - pubmed
Therefore , the biological sulfate reduction process in the column experiments was evaluated by combining classical analytical methods [ measuring heavy metal concentration , SO4 ( 2 -)-concentration , pH , dissolved organic carbon ( DOC ) ] with molecular methods [ denaturing gradient gel electrophoresis ( DGGE ) fingerprinting and phylogenetic sequence analysis ] based on either the 16S rRNA - gene or the dsr ( dissimilatory sulfite reductase ) gene , the latter being a specific biomarker for SRB . - pubmed
Intentional disruption of the lactate and HRC supplies resulted in an immediate stagnation of the ISMP processes and in a rapid release of precipitated metals into the column effluents . - pubmed
DGGE on the dsrB genes was successfully used to link the results of the ISMP process to the community composition of the sulfate reducing bacteria . - pubmed
Uric acid ( UA ) , peroxidase and total antioxidant status ( TAS ) were studied in both saliva and serum ; superoxide dismutase ( SOD ) was evaluated only in saliva . - pubmed
RESULTS : Twenty one weeks after SAS : mass index ( SG - HF>SG>CG , p<0.05 ) ; systolic pressure ( SG - HF= SG>CG , p<0.05 ) ; diastolic pressure ( SG - HF>SG>CG , p<0.05 ) ; systolic and diastolic meridional stress ( SG - HF>SG>CG , p<0.05 ) ; LV myocyte cross - sectional area ( SG - HF>SG>CG , p<0.05 ) and hydroxyproline content ( SG - HF>SG>CG , p<0.05 ) . - pubmed
DEVELOPMENT : Clinical trials that study the use of alteplase in the acute stroke treatment , especially the two phase III clinical trials carried out by the National Institute of Neurological Disorders and Stroke rt - PA Stroke Study Group , are described . - pubmed
Prior studies indicate that hLa is activated by phosphorylation of serine-366 by protein kinase CK2 , neutralizing a negative effect of a short basic motif ( SBM ) . - pubmed
These mutational oncogenic differences could lead to different therapeutic responses : nonmucinous BAC has been found to be sensitive to EGFR tyrosine kinase inhibitors , while mucinous BAC might be more responsive to taxane - based chemotherapy . - pubmed
Apoptosis was detected by flow cytometry , using annexin V and by cytological detection with fluorescein diacetate / propidium iodide staining . - pubmed
Without using density - gradient centrifugation , the bone marrow aspirate was poured into a dish ( 6.0 \times 10(5 ) nucleated cells / cm(2 ) ) with DMEM medium containing 10 % serum ( FCS or donor serum ) and basic fibroblast growth factor , and incubated at 37 degrees C under a 5 % CO ( 2 ) atmosphere . - pubmed
Herein , we have explored the role of 3 key components ( Apc , beta - catenin , and c - Myc ) of the Wnt pathway in the zonation of ammonia metabolizing enzymes . - pubmed
RESULTS : Under normal circumstances , the urea cycle enzyme carbamoylphosphate synthetase I ( CPS I ) is present in the periportal , intermediate , and the first few layers of the perivenous zone . - pubmed
Bile acids inhibit duodenal secretin expression via orphan nuclear receptor small heterodimer partner ( SHP ) . - pubmed
Small heterodimer partner ( SHP ) is an orphan nuclear receptor in which gene expression can be upregulated by bile acids . - pubmed
Here , we evaluated the regulation on duodenal secretin gene expression by SHP and selected bile acids , cholic acid ( CA ) and chenodeoxycholic acid ( CDCA ) . - pubmed
In vitro treatment of CDCA or fexaramine elevated the SHP transcript level and occupancy on secretin promoter . - pubmed
The increase in the SHP level , induced by bile acid treatment or overexpression , reduced secretin gene expression , whereas this gene inhibitory effect was reversed by silencing of endogenous SHP . - pubmed
A diet enriched with 5 % cholestyramine led to a decrease in SHP level and a corresponding increase in secretin expression . - pubmed
Overall , this study showed that bile acids via SHP inhibit duodenal secretin gene expression . - pubmed
Because secretin is a key hormone that stimulates bile flow in cholangiocytes , this pathway thus provides a novel means to modulate secretin - stimulated choleresis in response to intraduodenal bile acids . - pubmed
Role of phosphatidylinositol mannosides in the interaction between mycobacteria and DC - SIGN . - pubmed
Recently , we have shown that although the mannose caps of the mycobacterial surface glycolipid lipoarabinomannan ( ManLAM ) are essential for the binding to DC - SIGN , genetic removal of these caps did not diminish the interaction of whole mycobacteria with DC - SIGN and DCs . - pubmed
Here we investigated the role of the structurally related glycolipids phosphatidylinositol mannosides ( PIMs ) as possible ligands for DC - SIGN . - pubmed
In a binding assay with both synthetic and natural PIMs , DC - SIGN exhibited a high affinity for hexamannosylated PIM(6 ) , which contains terminal alpha(1 - ->2)-linked mannosyl residues identical to the mannose cap on ManLAM , but not for di- and tetramannosylated PIM(2 ) and PIM(4 ) , respectively . - pubmed
bovis bacillus Calmette - Guerin deficient in the production of PIM(6 ) ( Delta pimE ) was created , as well as a double knockout deficient in the production of both PIM(6 ) and the mannose caps on LAM ( Delta pimE Delta capA ) . - pubmed
The nematode eukaryotic translation initiation factor 4E / G complex works with a trans - spliced leader stem - loop to enable efficient translation of trimethylguanosine - capped RNAs . - pubmed
Heme oxygenase-1 ( HO-1 ) , an inducible enzyme , degrades heme to carbon monoxide ( CO ) , iron , and bilirubin . - pubmed
HO enzyme activity was reduced immediately following CO exposure , while expression of HO-1 protein was consistently upregulated in a time - dependent manner . - pubmed
We suggest that CO initially inhibits HO enzyme activity , whereas at later time points the inhibition is released and local CO generation is maintained by the activity of the endogenous HO enzyme . - pubmed
And the noninducible form of heme oxygenase , HO-2 , was not altered following CO administration . - pubmed
Patterns of iodine intake and urinary iodine concentrations during pregnancy and blood thyroid - stimulating hormone concentrations in the newborn progeny . - pubmed
Iodine nutrition was assessed by measuring urinary iodine concentration ( UIC ) and neonatal thyrotropin ( TSH ) . - pubmed
Plasma determinations included TSH , free thyroxine , thyroid peroxidase antibody , and thyroglobulin antibody . - pubmed
In addition , neonate TSH screening is not the best tool to assess whether the iodine status in populations is ideal . - pubmed
Maternal blood was collected at both necropsies and at weekly intervals for assay of glucose , insulin and leptin . - pubmed
Improvement in metformin and insulin utilisation in the Australian veteran population associated with quality use of medicines intervention programs . - pubmed
PURPOSE : To examine the impact of 2001 and 2005 quality use of medicines ( QUMs ) diabetes programs implemented by National Prescribing Service ( NPS ) on the prevalence of utilisation of metformin and insulin among the Australian diabetes veteran population . - pubmed
Prevalence of utilisation of metformin and insulin were assessed . - pubmed
RESULTS : Of the diabetes population , over 55 % has been dispensed metformin , and around 20 % insulin . - pubmed
Twenty - four months post the onset of second NPS diabetes intervention , there was 4.2 % relative increase in metformin use , and 13.5 % relative increase in insulin used concurrently with oral hypoglycaemics , compared to the estimates without the interventions . - pubmed
Density functional calculations were carried out to ascertain the origin of enantioselectivity in the brucine N - oxide ( BNO)-assisted enantioselective Pauson - Khand reaction ( PKR ) of norbornene with 2 - methyl-3 - butyn-2 - ol . - pubmed
Delaying ART initiation by at least 2 weeks after amphotericin B treatment may decrease the incidence of IRIS . - pubmed
Rst2 and Ste11 proteins were induced and localized to the nucleus of sam5 and sam7 mutants even under rich glucose conditions , indicating that the function of Pka1 was completely abolished by mutations . - pubmed
Pka1 - G441E and Pka1 - G441R mutant proteins reside in the cytoplasm , even under glucose - rich conditions , while wild - type Pka1 resides in the nucleus , indicating that the functionality of Pka1 is important for its nuclear localization . - pubmed
This is supported by the observation that the Pka1 - T356A mutant , which partially lacks Pka1 function , was localized to both the cytoplasm and the nucleus , but an active phosphomimetic Pka1 - T356D mutant prtotein was localized to the nucleus under glucose - rich conditions . - pubmed
In addition to the basal phosphorylation of Pka1 at T356 , hyperphosphorylation of Pka1 was observed under glucose - starved conditions , and such hyperphosphorylation was not observed in pka1 - G441E , pka1 - G441R , pka1 - T356A or pka1 - T356D mutants . - pubmed
Multiple cells of Deltaproteobacteria cluster SAR324 , Gammaproteobacteria clusters ARCTIC96BD-19 and Agg47 , and some Oceanospirillales from the lower mesopelagic contained ribulose-1,5 - bisphosphate carboxylase - oxygenase and sulfur oxidation genes . - pubmed
This research employed ( 29 ) Si and ( 13 ) C Cross - Polarisation / Magic Angle Spinning ( CP / MAS ) NMR spectroscopy to characterise the nature and amount of surface species of di - and trifunctional mercaptopropylsilane ( MPS ) bonded silica using supercritical carbon dioxide ( sc - CO ( 2 ) ) as a reaction solvent without additives ( co - solvent ) or catalysts . - pubmed
Sirius red staining was employed to detect the expression of myocardial collagen , and the myocardial collagen volume fraction ( CVF ) and perivascular collagen volume area ( PVCA ) were calculated . - pubmed
In the DOC group and ALI group , the CVF and PVCA were significantly increased ; the RA and Ang II levels in the plasma and ventricle were remarkably lowered when compared with the CON group . - pubmed
The RA and Ang II levels in the ventricle of the ALI group were significantly lower than those in the DOC group . - pubmed
Moreover , the expressions of ERK1 / 2 , PERK1 / 2 and MMP9 were the lowest in the CON group , but those in the ALI group were significantly reduced as compared to the DOC group . - pubmed
ALI can inhibit the DOCA induced myocardial fibrosis independent of its pressure - lowing effect , which may be related to the suppression of RA and Ang II production , inhibition of ERK1 / 2 phosphorylation and MMP9 expression in the heart . - pubmed
Approved drugs include tyrosine kinase inhibitors ( TKI ) such as sunitinib , sorafenib and pazopanib , vascular endothelial growth factor inhibitors such as bevacizumab , and mammalian target of rapamycin ( mTOR ) inhibitors such as temsirolimus and everolimus . - pubmed
Pharmacological inhibition of GSK-3 by SB216763 dose - dependently attenuated TGF - beta1 - induced expression of these myofibroblasts markers . - pubmed
Moreover , silencing of GSK-3 by siRNA or pharmacological inhibition by CT / CHIR99021 fully inhibited the TGF - beta1 - induced expression of alpha - sm - actin and fibronectin . - pubmed
Neither smad , NF - kappaB nor ERK1 / 2 were involved in the inhibitory actions of GSK-3 inhibition by SB126763 on myofibroblast differentiation . - pubmed
Rather , SB216763 increased the phosphorylation of CREB , which in its phosphorylated form acts as a functional antagonist of TGF - beta / smad signalling . - pubmed
OBJECTIVES : Hepcidin , a peptide hormone released by hepatocytes into circulation is the main regulator of dietary iron absorption and cellular iron release . - pubmed
The effects of ( -)-noradrenaline , mediated through beta1 adrenoceptors ( beta2 adrenoceptors blocked with ICI118551 ) , and ( -)-adrenaline , mediated through beta2 adrenoceptors ( beta1 adrenoceptors blocked with CGP20712A ) , were assessed in the absence and presence of PDE inhibitors . - pubmed
Concurrent therapy with a PDE3 blocker and metoprolol could conceivably facilitate cardiostimulation evoked by adrenaline through beta2 adrenoceptors . - pubmed
RESULTS : Both the doses of vanillin reduced the immobility duration in TST as well as in FST . - pubmed
But the reduction of immobility in FST did not show statistically significant reduction in immobility in the groups treated with vanillin when compared with control . - pubmed
To further study the functional process of DHS regulated bone metabolism , the objective of this study was to evaluate the effects of DHS on cortical bone and its alterations on gene expressions of osteogenic growth factors and transcription factors as a function of time . - pubmed
A lincRNA - DYNLRB2 - 2 / GPR119 / GLP-1R / ABCA1 - dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis . - pubmed
Accumulated evidence shows that G protein - coupled receptor 119 ( GPR119 ) plays a key role in glucose and lipid metabolism . - pubmed
In hd - PS matched cohorts , the overall MI rate difference ( RD ) comparing prasugrel with clopidogrel was -23.1 ( 95 % confidence interval [ CI ] -62.8 - 16.7 ) events per 1,000 person - years and RDs were -0.5 ( -12.9 - 11.9 ) and -2.8 ( -13.2 - 7.6 ) for a composite bleed event outcome and death from any cause , respectively . - pubmed
RATIONALE : Dihydropyridines are widely used for the treatment of several cardiac diseases due to their blocking activity on L - type Ca ( 2 + ) channels and their renowned antioxidant properties . - pubmed
METHODS : We synthesized six novel dihydropyridine molecules and performed docking studies on the binding site of the L - type Ca ( 2 + ) channel . - pubmed
The Ca ( 2 + ) channel - blocking activity was evaluated by confocal microscopy on fluo-3AM loaded cardiomyocytes , as well as using patch clamp experiments . - pubmed
RESULTS : Our docking studies show that a novel compound with 3 - OH substitution inserts into the active binding site of the L - type Ca ( 2 + ) channel previously described for nitrendipine . - pubmed
In biochemical assays , the novel meta - OH group in the aryl in C4 showed a high blocking effect on L - type Ca ( 2 + ) channel as opposed to para - substituted compounds . - pubmed
CONCLUSIONS : Only substitutions in C2 , C3 and C5 of the aryl ring render dihydropyridine compounds with the capacity of blocking LTCC . - pubmed
Based on our docking studies , we postulate that the antioxidant activity requires a larger group than the meta - OH substitution in C2 , C3 or C5 of the dihydropyridine ring . - pubmed
In the current study , we measured the correlations among physical activity , nutrient intake , smoking , body - mass index ( BMI ) , and age with the concentration of triglycerides , cholesterol , phospholipids , and apolipoproteins ( ApoA1 , ApoA2 and ApoB ) in subfractions of LDL and HDL in 265 healthy working men . - pubmed
Concentrations of cholesterol , phospholipids , and ApoB in small , dense atherogenic LDL particles ( sdLDL ) correlated negatively ( p<0.001 ) with those of cholesterol , phospholipids , and ApoA1 in HDL2 , respectively . - pubmed
Age correlated positively with sdLDL while increasing BMI correlated with an atherogenic shift of cholesterol , phospholipids , and ApoB from large , buoyant LDL ( lbLDL ) to sdLDL and decreasing concentrations of HDL2 constituents . - pubmed
Physical activity and alcohol intake correlated negatively with sdLDL constituents and positively with HDL2 components . - pubmed
Consumption of monounsaturated fatty acids ( MUFA ) correlated with a lower ratio of sdLDL to HDL2 cholesterol . - pubmed
A multivariate model based on the product age - by - MELD ( OR = 0.81 ; P = 0.013 ) , delta eosinophils ( OR = 1.5 ; P = 0.002 ) and mean tacrolimus trough concentrations & lt;6 ng / ml within the prior 4 days ( OR = 11.4 ; P = 0.047 ) had an AUROC = 0.84 to diagnose moderate - severe histological ACR . - pubmed
Long - Term Effects of Novel Combination Coating Anti - CD34 Antibody Applied on Sirolimus - Eluting Stents . - pubmed
Moreover , we showed that propyl pyrazole triol ( an ERalpha agonist ) stimulates the phosphatidyl inositol 3 - kinase ( PI3 K ) pathway specifically in POMC progenitor neurons , and that blockade of PI3 K attenuates propyl pyrazole triol - induced activation of POMC neurons . - pubmed
From the morula stage onward , in vitro fertilized embryos were cultured in the presence of cell - permeable small molecules targeting nine principal signaling pathway components , including TGFbeta1 , BMP , EGF , VEGF , PDGF , FGF , cAMP , PI3 K , and JAK signals . - pubmed
TGFbeta1 , BMP , and cAMP and combined VEGF / PDGF / FGF signals did not affect blastocyst development while PI3 K was important for ICM growth but did not alter lineage - specific gene expression . - pubmed
Stimulating cAMP specifically increased NANOG expression , while combined VEGF / PDGF / FGF inhibition up - regulated epiblast and hypoblast markers . - pubmed
The strongest effects were observed by suppressing JAK1 / 2 signaling with AZD1480 . - pubmed
We found that tyrosine ( Y ) 705 - phosphorylated STAT3 ( pSTAT3(Y705 ) ) was restricted to ICM nuclei , where it colocalized with SOX2 and NANOG . - pubmed
The concentration - dependent internalization / bio - uptake of Cr2O3 NPs may have contributed to the enhanced production of anti oxidant enzyme , superoxide dismutase to counteract the oxidative stress , which in turn resulted in observed chromosomal aberrations and cytogenetic effects . - pubmed
To produce red pigments , birds ingest yellow carotenoids and endogenously convert them into red ketocarotenoids via an oxidation reaction catalyzed by a previously unknown ketolase [ 4 - 6 ] . - pubmed
Lastly , we present evidence that CYP2J19 is involved in ketocarotenoid formation in the retina . - pubmed
Without betamethasone , PEEP increased FRC recruitment and CDL Surfactant did not further increase FRC , but significantly increased CDL Betamethasone abolished the benefit of PEEP on FRC , but surfactant counteracted this effect of betamethasone . - pubmed
Procaine also inhibited JAK2 and STAT3 expression in both mRNA ( p<0.05 ) and protein levels . - pubmed
Overexpression of JAK2 increased STAT3 level and reversed the improvement effects of procaine in pain behaviors ( p<0.01 ) . - pubmed
OBJECTIVE : The toxicity of anti - cancer chemotherapeutic agents can be reduced by associating these compounds , such as the anti - proliferative agent paclitaxel , with a cholesterol - rich nanoemulsion ( LDE ) that mimics the lipid composition of low - density lipoprotein ( LDL ) . - pubmed
Familial Hyperaldosteronism type 1 ( FH1 ) or dexamethasone suppressible hyperaldosteronism , FH2 , the most common form of as yet unknown cause(s ) , and FH3 . - pubmed
FH3 is due to activating mutations of the potassium channel gene KCNJ5 that increase constitutive and angiotensin II - induced aldosterone synthesis . - pubmed
The enzyme was purified to homogeneity by ammonium sulfate precipitation , Sephadex G-75 gel filtration , Mono - Q anion - exchange chromatography and ultrafiltration , with a 12.70 - fold increase in specific activity and 20.29 % recovery . - pubmed
In photosystem II ( PSII ) , water oxidation occurs at a Mn4CaO5 cluster and results in production of molecular oxygen . - pubmed
The calculated frequencies were compared to S2 - minus - S1 reaction - induced Fourier transform infrared spectra acquired from chloride- , bromide- , or nitrate - containing PSII samples , which were active in oxygen evolution . - pubmed
These results support the conclusion that an internal hydronium ion and chloride play a direct role in an internal proton transfer event during the S1 - to - S2 transition . - pubmed
Phase - II sulfate conjugation of phenolic compounds is generally thought to result in their inactivation . - pubmed
Calixarene - Based Copper ( I ) Complexes as Models for Monocopper Sites in Enzymes . - pubmed
When substoichiometric rhombohedral alpha - SrUO4 - x , in space group R3 m with disordered in - plane oxygen defects , is heated above 200 C in a hydrogen atmosphere it undergoes a first order phase transformation to a ( disordered ) triclinic polymorph , delta - SrUO4 - x , in space group P1 . - pubmed
Although CCK8 and colony - formation assays showed that SIRT2 overexpression marginally promoted proliferation in GC cell lines , SIRT2 knockdown or treatment with SirReal2 decreased the migration and invasion of GC cells . - pubmed
We demonstrated both in vitro and in vivo that SirReal2 could inhibit the deacetylation activity of SIRT2 and its downstream target PEPCK1 , which is related to mitochondrial metabolism and RAS / ERK / JNK / MMP-9 pathway . - pubmed
SirReal2 therefore shows promise as a new drug candidate for GC therapy . - pubmed
In particular , the transition pore opening induced by AGE was due to the rearrangement of the mitochondrial membranes following the increased activity of the K +/ H + exchanger . - pubmed
Pab1 consists of four RNA recognition motifs ( RRM ) , a proline - rich domain ( P ) and a carboxy - terminal ( C ) domain . - pubmed
However , hyperoxia decreased cardiac tissue three - nitrotyrosine formation ( P & lt ; 0.001 ) and inducible nitric oxide synthase expression ( P = 0.016 ) . - pubmed
Gemcitabine plus S-1 ( GS ) is commonly used to treat advanced pancreatic cancer ( APC ) in Asia . - pubmed
Here we demonstrated forty - seven filamentous fungal isolates were obtained from eight soil samples using a new screening strategy with both triphenyltetrazolium chloride ( TTC ) , a redox indicator used for testing oil presence , and cerulenin , an inhibitor of fatty acid synthase ( FAS ) , supplemented in screening medium . - pubmed
1 ) We found that the administration of recombinant fetuin B aggravated hepatic lipid accumulation caused by free fatty acids ( FFAs ) or high fat diet , in vivo and in vitro . - pubmed
It lowered the phosphorylated AMPK levels and activated the LXR - SREBP1c pathway , accompanied by the downregulation of fatty acid oxidation and upregulation of lipogenesis . - pubmed
It enhanced phosphorylated AMPK and depressed the LXR - SREBP1c pathway , accompanied by upregulation of fatty acid oxidation and downregulation of lipogenesis . - pubmed
On one hand , mycolactone induces analgesia by targeting type-2 angiotensin II receptors ( AT2R ) , causing cellular hyperpolarization and neuron desensitization . - pubmed
Her serum creatine kinase was 6,300 U / L. Pitavastatin was stopped , but her symptoms deteriorated , and cardiac congestion appeared . - pubmed
Three SNPs caused amino acid changes in NAD - ME1 in CSR12906 , N299S , S488N and S544N. - pubmed
Ribavirin plasma trough concentrations were determined at week 4 of therapy using a liquid chromatography / tandem mass spectrometry ( LC - MS / MS ) . - pubmed
For clinical outcomes , sustained virological response ( SVR ) , liver function tests ( ALT and AST ) , total bilirubin , albumin , serum creatinine , hemoglobin , leukocyte count , and platelet count were measured . - pubmed
RESULTS : Concerning RBV pharmacokinetics , ABCB1 2677 G > T SNP and ABCB11 1331 T > C SNP were statistically associated with RBV Ctrough levels after 4 weeks of therapy . - pubmed
SLC28A2 - 146 A > T SNP has not showed any statistically significant association with RBV plasma levels or response . - pubmed
CONCLUSION : SNP genotyping for ABCB1 and ABCB11 genes can help in better personalized medicine for maximizing response for ribavirin as explored by the significant association between polymorphism in ABCB1 and ABCB11 genes and ribavirin pharmacokinetics and the significant association of ABCB11 1331 T > C SNP with clinical response . - pubmed
The aim of this study was to examine whether combining a novel BRD4 inhibitor , ITH-47 , with the antimitotic estradiol analogue , ESE-15 - ol , would have a synergistic effect on inhibiting the growth of two different breast cancer cell lines in vitro . - pubmed
Our docking and molecular dynamics studies showed that compared to JQ1 , ITH-47 showed a similar binding mode with hydrogen bonds forming between the ligand nitrogens of the pyrazole , ASN99 , and water of the BRD4 protein . - pubmed
Of these pendant - bearing GNGs ( P - GNGs ) , three detergents ( GNG-2,14 , GNG-3,13 and GNG-3,14 ) were not only notably better than both DDM ( a gold standard detergent ) and the previously described GNGs at stabilizing all six membrane proteins tested here , but were also as efficient as DDM at membrane protein extraction . - pubmed
The regression model showing an acceptable fit to the experimental data ( adjusted R2 0.84 ) for AFB1 as a function of grains steeping degree , temperature and time of germination . - pubmed
An amino - terminal point mutation increases EAAT2 anion currents without affecting glutamate transport rates . - pubmed
Excitatory amino acid transporters ( EAATs ) are prototypic dual function proteins that function as coupled glutamate / Na+/H+/K+ transporters and as anion - selective channels . - pubmed
In contrast , changes in selectivity made gluconate permeant in L46P EAAT2 , and non - stationary noise analysis revealed slightly increased unitary current amplitudes in mutant EAAT2 anion channels . - pubmed
We used unitary current amplitudes and individual transport rates to quantify absolute open probabilities of EAAT2 anion channels from ratios of anion currents by glutamate uptake currents . - pubmed
In the present study , we evaluated four small molecule affinity - based probes based on agarose - immobilized benzamidine ( ABA ) , O - Phospho - L - Tyrosine ( pTYR ) , 8 - Amino - hexyl - cAMP ( cAMP ) , or 8 - Amino - hexyl - ATP ( ATP ) for their ability to remove high - abundant proteins such as serum albumin from plasma samples thereby enabling the detection of medium - to - low abundant proteins in plasma samples by mass spectrometry - based proteomics . - pubmed
To clarify whether PXR and CAR regulate various genes involved in drug metabolism of high - altitude hypoxia , we investigated the expression of CYP1A2 , CYP2C9 , CYP2E1 , CYP3A4 , and UGT1A1 using a dual - luciferase reporter assay after treatment with Ketoconazole ( KCZ ) and Retinoic acid ( RA ) , or silenced PXR and CAR gene expression . - pubmed
Activation of PXR and CAR in cells treated with 6 -( 4 - chlorophenyl )- imidazo ( 2,1 - b ) thiazole-5 - carbaldehyde ( CITCO ) and rifampicin ( Rif ) resulted in the enhancement of CYP450 isoforms , UGT1A1 , PXR , and CAR . - pubmed
The level of membrane phosphatidylserine , a target of the scavenger receptors , which initiates removal of damaged cells and modified lipoproteins from the circulation was also elevated . - pubmed
BACKGROUND : Prophylactic pancreatic stent placement ( PSP ) and rectal indomethacin suppository are recommended to prevent post - endoscopic retrograde cholangiopancreatography ( ERCP ) pancreatitis ( PEP ) in high - risk cases . - pubmed
Clinical trials on the use of nitroglycerin to reduce PEP have reached no definitive conclusion . - pubmed
The three treatment groups were : the placebo group , the indomethacin + nitroglycerin group , and the PSP group . - pubmed
The rate of PEP in the three study groups placebo group , indomethacin + nitroglycerin group and the PSP group was 19.3 % , 5.1 % , and 12.1 % , respectively . - pubmed
CONCLUSIONS : The risk of post - ERCP pancreatitis in the indomethacin + nitroglycerin group was 7 % lower than that in the PSP . - pubmed
Indomethacin plus nitroglycerin can avoid the need for PSP in the prevention of post - ERCP pancreatitis . - pubmed
Growth in methanol - containing medium results in dramatic induction of genes in the alcohol oxidation pathway including alcohol oxidase ( AOX ) , formaldehyde dehydrogenase ( FLD ) , and dihydroxyacetone synthase ( DHAS ) . - pubmed
17beta - Estradiol ( E2 ) confers neuroprotection in preclinical models of spinal cord injury when administered systemically . - pubmed
The goal of this study was to apply E2 locally to the injured spinal cord for a sustained duration using poly ( pro - E2 ) film biomaterials . - pubmed
To increase the E2 dose released , bolus - releasing poly ( pro - E2 ) films were fabricated by incorporating unbound E2 into the poly ( pro - E2 ) films . - pubmed
However , compared to the injured controls , bolus - releasing poly ( pro - E2 ) films did not significantly enhance neuroprotection or limit inflammation at either 7 or 21 days post - injury . - pubmed
The fluorescent peptide substrate KKRAARAC(sulfobenzo - furazan ) SNVFS - amide was used to measure kinase activity . - pubmed
We were able to remove the contaminating antibody from the anti - calmodulin by passing the affinity - purified mixture over an antigen - free Affi - Prep 10 column . - pubmed
The antipruritic effect of 3 days of treatment with the non - sedative H1 antagonist terfenadine ( 60 mg b.i.d . ) - pubmed
Amiloride inhibits the protein tyrosine kinases associated with the cellular and the transforming src - gene products . - pubmed
In immunocomplexes , TBR - IgG serves as an artificial but specific phosphate acceptor . - pubmed
The phosphate incorporation into TBR - IgG is a time- and temperature - dependent process . - pubmed
In the presence of amiloride the TBR - IgG phosphorylation is reduced . - pubmed
Half - maximal inhibition of the tyrosine kinase occurs at 300 microM amiloride and is competitive with respect to ATP . - pubmed
Viral pp60src kinase of transformed cells is more sensitive to amiloride ( IC50 : 50 - 100 microM ) . - pubmed
Furthermore , normal cellular tyrosine kinases are to a lesser extent inhibited by amiloride as compared to the transforming viral pp60src kinase . - pubmed
These results may indicate different amiloride - sensitive forms of cellular pp60src kinases . - pubmed
Furthermore , MAL transplantation is associated with less increased levels of plasma ammonia during acute liver ischemia . - pubmed
The compound CY 222 was administered as 1.4 mg / kg ( group A ) by IV injection immediately after start of t - PA infusion ( 0.125 and 0.250 mg / kg ) and then hourly during the 4 hours of infusion of the thrombolytic ; results were compared with those obtained in the absence of CY 222 ( group B ) . - pubmed
The conclusion is reached of the interest of CY 222 in combination with t - PA , the CY 222 acting not only on coagulation by also on thrombolysis by potentiating the effect of t - PA . - pubmed
All alpha subunits bind guanine nucleotides and are ADP - ribosylated by either pertussis toxin ( Gi , transducin , Go ) or cholera toxin ( Gs , Gi , transducin ) . - pubmed
Thus , transducin and Gi , which have the highest degree of sequence homology , are also ADP - ribosylated by both toxins . - pubmed
While the gamma subunits of Gs , Gi , and Go have identical electrophoretic mobility in SDS gels , they differ significantly in this respect from the gamma subunit of transducin . - pubmed
[ Auranofin and aurothiomalate sodium : a comparative review ( and II ) Efficacy , tolerance and safety ] . - pubmed
Eight responders were monitored for F - AT throughout cyclophosphamide chemotherapy . - pubmed
Failure to accumulate protein methyl esters , either in vitro in in vivo , was most likely due to the presence of a very active protein methylesterase . - pubmed
The turnover of protein methyl esters was primarily enzymatic , since no [ 3H ] methanol was formed when ACTH was incubated with AdoMet and purified protein carboxyl methyltransferase . - pubmed
Utilizing [ 3H ] methanol formation as an index of the rate of protein methylation - demethylation would yield minimal values , since this compound was oxidized via alcohol dehydrogenase or oxidase in these cells . - pubmed
The isolated homoaconitic acid was also identified as a substrate for a purified enzyme preparation of homoaconitase . - pubmed
These three HMG ' s from embryonic skeletal muscle and erythrocytes also comigrated on two - dimensional gels , employing an acid - urea system in the first dimension and an NaDodSO4 system in the second . - pubmed
Interpretation of the two - dimensional gels suggested that the two low molecular weight proteins of this triplet arose from the HMG 2 band of the acid - urea gels . - pubmed
On the other hand , HLA - Bw52 - Dw12 haplotype which was reported to be in strong linkage disequilibrium with an immune suppression gene for schistosomal adult worm antigen was significantly decreased ( corrected P less than 0.005 ) . - pubmed
Influence of endotoxin treatment on dexamethasone induction of hepatic phosphoenolpyruvate carboxykinase . - pubmed
Furthermore , the induction of hepatic PEPCK by dexamethasone was inhibited by 1 h after endotoxin treatment , with enzyme activity falling to basal , noninduced levels by 6 h posttreatment . - pubmed
Additional , but fewer zinc - containing neurons were located in layers II , III and deep layer VI of the ipsilateral neocortex . - pubmed
In the cingulate cortex , zinc - containing neurons with striatal projections were found predominantly in the ipsilateral layers II and III , with only few neurons in the ipsilateral layer VI and in the contralateral layers II , III and VI . - pubmed
Zinc has been found to modulate the response of many ligand- and voltage - gated ion channels , including both GABA receptors and NMDA- , AMPA- and kainate - type glutamate receptors . - pubmed
In this report , we demonstrate that a single amino acid mutation of a Tyr to a Cys at position 723 ( Y723C ) in the TM cytoplasmic domain of CP - MAC is the principal determinant for the increased expression of envelope glycoproteins on the cell surface . - pubmed
Substituting other amino acids , including Ala , Ile , and Ser , at this position produced increases in surface envelope glycoproteins that were similar to that observed for the Cys substitution , while a Tyr - to - Phe mutation produced a smaller increase . - pubmed
To test this hypothesis , confluent endothelial cell monolayers were exposed for 24 h to 500 or 1,000 U of TNF per milliliter of culture medium together with 20 microCi Na2 35SO4 . - pubmed
HPLC anion - exchange separation of proteoglycans secreted into media of control as well as TNF - treated cultures revealed one major peak ( representing 95 % of total radioactivity ) and one minor peak ( representing 5 % of total radioactivity ) , which eluted at 0.6 and 0.9 M NaCl , respectively . - pubmed
TNF treatment caused a dose - dependent decrease in mRNA levels for the core proteins of perlecan , a major heparan sulfate proteoglycan ( HSPG ) , and biglycan in endothelial cultures . - pubmed
The carboxyl terminus - truncated cadherin ( nonfunctional cadherin ) has no cell adhesion activity probably because of its failure to associate with cytoplasmic proteins called alpha and beta catenin . - pubmed
Taken together , we conclude that in the formation of the cadherin - catenin complex , the mechanical association of alpha catenin , especially its carboxy - terminal half , with E - cadherin is a key step for the cadherin - mediated cell adhesion . - pubmed
This article reports the complete structure of X , which was determined from the FAB - MS and 2D NMR analysis of the peracetylated LOS - III . - pubmed
Sequential extraction was performed utilizing 1 ) 0.1 % Triton X-100 , 2 ) 4 M guanidine - HCl , and 3 ) papain digestion , to release species of hyaluronan progressively more tightly associated with tissue . - pubmed
Hemimandibles were osteotomized perpendicular to the long axis between the third and fourth premolars , and segments were stabilized with 1 of 5 interdental fixation apparatuses : Erich arch bar ( EAB , n = 6 ) ; Stout loop ( SL , n = 6 ) ; acrylic ( A , n = 6 ) ; Stout loop and acrylic ( SLA , n = 24 ) ; and Erich arch bar and acrylic ( EABA , n = 6 ) . - pubmed
DNAase I and micrococcal nuclease reveal drug - induced footprints with the central sequences ATAT , TATA and TTAA , but not AATT , suggesting that the ligand binds to the dinucleotide TpA. - pubmed
Intrinsic activity was demonstrated in vitro against a variety of phosphotyrosine - containing substrates including BIRK , the autophosphorylated cytoplasmic kinase domain of the insulin receptor beta subunit . - pubmed
The major criteria for purity of the bmv fraction are : ( i ) It is enriched 15 - fold for a known plasma membrane marker ( immobilization antigen ) while the marker activities for other membranes were all decreased . - pubmed
The protein banding pattern of bmv is generally similar to cmv on SDS - PAGE . - pubmed
( ii ) It contains a vanadate - sensitive Ca ( 2 +)- ATPase activity that has been suggested to be a plasma membrane Ca2 + pump . - pubmed
( iii ) The phospholipid , fatty acid , and sterol composition of the bmv fraction are indicative of plasma membranes because they are qualitatively similar to cmv . - pubmed
This purified bmv preparation is useful for studying the role of the body plasma membrane in Ca2 + regulation , sensory transduction , protein and lipid trafficking , and plasma membrane fusion events . - pubmed
The effect of L - RNA was dependent of the integrity of the polynucleotide chain and was not due to LPS contamination since its ability to induce MNCF and IL-8 release was strongly reduced by RNase but was not affected by DNase or polymyxin B. - pubmed
The demonstration that actinomycin - D and cycloheximide inhibited the release of MNCF and IL-8 by L - RNA - stimulated macrophages confirms this assumption . - pubmed
Fractionation of the total L - RNA by centrifugation on a 5 - 20 % sucrose gradient showed that the L - RNA which sediments in the 4 - 5S region of the gradient is the only fraction capable of inducing the release of MNCF from naive macrophages . - pubmed
High - resolution crystal structure of magnesium ( MgII)-iron ( FeII ) hybrid hemoglobin with liganded beta subunits . - pubmed
The deoxy hybrid has virtually the same structure as that of the native deoxy HbA. - pubmed
Both the deoxy and CO - liganded hybrids assumed a T quaternary structure characteristic of native deoxy HbA. - pubmed
No significant structural difference was found between the alpha subunits of the CO - liganded hybrid and deoxy HbA , while in the beta subunit , significant tertiary structural changes were confined to the heme pocket . - pubmed
On the other hand , when the two structures are compared with superposition of hemes , the nearest neighbors of CO ( Fe , E11 Val and E7 His ) have shifted nearly to the same positions as those in COHbA. - pubmed
The persistence of this photoproduct and its high neuroactivity , resulting from blocking the lambda - aminobutyric acid - gated chloride channel , suggest that it may be a significant contributor to the effectiveness of fipronil . - pubmed
Based upon the CAPS , a standardized post - traumatic stress disorder ( PTSD ) interview , findings from a cohort involving both subchronic and chronic traumatic stress marginally favored brofaromine over placebo ; however , not to a statistically significant degree . - pubmed
Optical densities of 5HT IR and VIP IR were increased by MAOI treatment . - pubmed
Phospholipase C - delta1 and oxytocin receptor signalling : evidence of its role as an effector . - pubmed
Although the oxytocin receptor modulates intracellular Ca2 + ion levels in myometrium , the identities of signal molecules have not been clearly clarified . - pubmed
PLC - delta1 activity in the three - component preparation , as well as PLC activity in solubilized membranes , was increased by oxytocin in the presence of Ca2 + and activated Ghalpha ( GTP - bound Ghalpha ) . - pubmed
Furthermore the stimulated PLC - delta1 activity resulting from activation of Ghalpha via the oxytocin receptor was significantly attenuated by the selective oxytocin antagonist desGly - NH2d ( CH2 ) 5 [ Tyr ( Me ) 2,Thr4 ] ornithine vasotocin or GDP . - pubmed
These results indicate that PLC - delta1 is the effector for Ghalpha - mediated oxytocin receptor signalling . - pubmed
Local delivery of cefazolin is desired in conditions such as osteomyelitis , soft - tissue infection and for prevention of post - surgical infections . - pubmed
Prolonged reversible nerve blockade required in a number of clinical situations involving acute or chronic pain such as post - surgical pain following herniorrhaphy and thoracotomy can be achieved with local delivery of bupivacaine . - pubmed
Therefore , P(FAD - SA , 50:50 w / w ) may not be undergoing surface erosion , and the diffusion and dissolution properties of the drug in addition to erosion characteristics of the polyanhydride appear to play a role in drug release . - pubmed
Cefazolin implants released the drug for a sufficient duration for osteomyelitis and soft - tissue infection but the release was more prolonged than required for prevention of post - surgical wound infection . - pubmed
, one encoding the G6PD , a major enzyme regenerating anti - alkylating GSH , and the other encoding ALDH1 , which has a high activity for oxidation of aldophosphamide derived from 4HC . - pubmed
We believe that there are sufficient arguments to support controlled iron supplementation in all athletes with low serum ferritin levels . - pubmed
Serum leptin levels rose 2- to 10 - fold , with peak increases seen 8 - 16 h after injection of CPA ( P & lt ; 0.05 ) . - pubmed
In contrast , CPA did not alter steady state levels of adipose tissue leptin mRNA . - pubmed
Supporting the idea that stimulation of leptin production is A1AR mediated , pretreatment with the A1AR antagonist 8 - cyclopentyl-1,3 - dipropylxanthine completely blocked increases in leptin levels after DPY treatment . - pubmed
In cultures , CPA treatment increased leptin secretion by 37 % ( P & lt ; 0.05 ) . - pubmed
Carboxyatractylate ( CAtr ) , an inhibitor of AAC , completely inhibits oleate- or laurate - stimulated oxygen consumption linked to succinate oxidation , while GDP , an inhibitor of PUMP , caused only a 30 % inhibition . - pubmed
These results show that the succinate - dependent H ( 2 ) O ( 2 ) formation occurs at the level of Complex I by a reverse electron transport . - pubmed
The anionic form of FFA appears to be shuttled by AAC rather than PUMP . - pubmed
Galactosylation of thiol group by beta - galactosidase . - pubmed
beta - Galactosidase catalyzed beta - galactosylation not only of a hydroxyl group but also of a thiol group in the condensation reaction of D - galactose and 2 - mercaptoethanol . - pubmed
Serum aspartate aminotransferase activity , serum and milk betahydroxybutyric acid concentrations , and milk fat - to - protein ratios were often elevated before the diagnosis of DA , and these variables may be used to predict DA . - pubmed
Two novel macromolecular MRI contrast agents based upon generation-6 polyamidoamine dendrimers ( G6 ) of presumed similar molecular size , but of different molecular weight , were compared in terms of their blood retention , tissue distribution , and renal excretion . - pubmed
The G6A --( 1B4M - Gd )( 192 ) showed higher signal intensity in the kidney on the dynamic MR images and brighter kidney images than G6E --( 1B4M - Gd )( 256 ) . - pubmed
In conclusion , the G6A --( 1B4M - Gd )( 192 ) was observed to go through glomerular filtration more efficiently than G6E --( 1B4M - Gd )( 256 ) resulting faster clearance from the blood and higher renal accumulation , even though both of G6 - -1B4 M conjugates have almost similar molecular size and same chemical structure . - pubmed
Role of estrogen receptor beta in estrogen action . - pubmed
The importance of androgen in females was never in doubt , it is after all the precursor of estrogen as the substrate for aromatase , the enzyme that produces estrogen . - pubmed
In addition , the tissue distribution of androgen receptors suggests that androgens themselves are important in the ovary , uterus , breast , and brain . - pubmed
OBJECTIVES : Tapping mode atomic force microscopy ( AFM ) was used to investigate the surface changes of collagen exposed to phosphoric acid treatment . - pubmed
Delayed cerebral edema and fatal coma after minor head trauma : role of the CACNA1A calcium channel subunit gene and relationship with familial hemiplegic migraine . - pubmed
Mutations in the CACNA1A calcium channel subunit gene on chromosome 19 are associated with a wide spectrum of mutation - specific episodic and chronic neurological disorders , including FHM with or without coma . - pubmed
In all three subjects with delayed severe edema , we found a C - to - T substitution resulting in the substitution of serine for lysine at codon 218 ( S218L ) in the CACNA1A gene . - pubmed
We conclude that the novel S218L mutation in the CACNA1A calcium channel subunit gene is involved in FHM and delayed fatal cerebral edema and coma after minor head trauma . - pubmed
Magnetic resonance imaging ( MRI ) demonstrated a reduction in the size of T2 high lesions and gadolinium enhancement in the thigh muscles in one case . - pubmed
The in vitro responsiveness of PMNs stimulated with fMLP or PAF was inversely correlated with indices of in vivo glycemic control ( fasting plasma glucose and glycated - hemoglobin levels ) . - pubmed
The applicability of this hybrid approach was evaluated by calculating the absolute free energy of binding and free - energy changes for amino acid substitutions in the complex between the U1A spliceosomal protein and its cognate RNA hairpin . - pubmed
Cyclooxygenase-2 ( COX-2 ) is an inducible enzyme homologous to COX-1 that is responsible for production of prostaglandins at sites of inflammation . - pubmed
PURPOSE : To determine if an association exists between postdilation restenosis and heme oxygenase-1 ( HO-1 ) , which is induced by balloon injury and inhibits neointimal formation through the action of endogenous carbon monoxide . - pubmed
A dinucleotide repeat in the promoter region of the HO-1 gene shows a length polymorphism that modulates the level of gene transcription . - pubmed
Blood levels of Ca , P , alkaline phosphatase , aluminium , iPTH and dialysis doses were measured every 4 months during the two years of follow - up . - pubmed
Here we report that APE1 has a DNA exonuclease activity on mismatched deoxyribonucleotides at the 3 ' termini of nicked or gapped DNA molecules . - pubmed
The exonuclease activity of APE1 can remove the anti - HIV nucleoside analogues 3 '- azido-3 '- deoxythymidine and 2 ' , 3 '- didehydro-2 ' , 3 '- dideoxythymidine from DNA , suggesting that APE1 might have an impact on the therapeutic index of antiviral compounds in this category . - pubmed
This oscillation parallels the 24 - min periodicity observed for the oxidation of NADH by the external plasma membrane NADH oxidase . - pubmed
The serotonin transporter ( 5HTT ) is the molecule responsible for the high - affinity reuptake of 5HT from the synaptic cleft . - pubmed
Among the 37 cases , 35 ( 94.6 % ) had total bile acids elevation , 32 ( 86.5 % ) had serum cholesterol elevation and 32 ( 86.5 % ) serum IgM elevation , 34 ( 91.9 % ) were positive for anti - mitochondrial antibody ( AMA ) and/or AMA - M2 . - pubmed
NONOates regulate KCl cotransporter-1 and - 3 mRNA expression in vascular smooth muscle cells . - pubmed
In this study , we used NONOates as rapid and slow NO releasers to provide direct evidence implicating NO as a regulator of KCC3 gene expression at the mRNA level . - pubmed
In addition , we used the expression of KCC3 mRNA to further investigate the mechanism of action of these NO donors at the cellular level . - pubmed
Treatment of VSMCs with rapid NO releasers , like NOC-5 and NOC-9 , as well as with the direct NO - independent soluble guanylyl cyclase ( sGC ) stimulator YC-1 , acutely increased KCC3 mRNA expression in a concentration- and time - dependent manner . - pubmed
The slow NO releaser NOC-18 had no effect on KCC3 gene expression . - pubmed
A specific NO scavenger completely prevented the NONOate - induced KCC3 mRNA expression . - pubmed
Inhibition of sGC with LY-83583 blocked the NONOate- and YC-1 - induced KCC3 mRNA expression . - pubmed
Lovastatin inhibits bone marrow - derived dendritic cell maturation and upregulates proinflammatory cytokine production . - pubmed
Lovastatin inhibited MHC class II and CD40 expression on DC in a dose - dependent manner , but had lesser effects on CD16 , CD80 , CD86 , and CD11b expression . - pubmed
Nuclear extracts of lovastatin treated DC had decreased NF - kappaB DNA binding activity . - pubmed
Lovastatin up - regulated DC pro - inflammatory cytokine production induced by LPS as measured by intracellular cytokine staining , ELISA and cDNA microarrays . - pubmed
These results indicate that lovastatin may inhibit BM - DC maturation and up - regulate cytokine production through a mevalonate dependent pathway , and may cause adverse effects on either innate or adaptive immunity . - pubmed
Also , the use of the radiopharmaceuticals , radioactive thallium chloride-201 , methylene diphosphonate labelled with technetium-99 m , somatostatin receptors labelled with indium-111 , diagnostic procedures with the PET camera , the labelled antibodies to CEA with technetium-99 m and also the importance of the MRI and the US techniques are mentioned . - pubmed
After rhG - CSF , there were increases both in TF antigen ( TF : Ag ) ( P=0.01 ) and TF procoagulant activity ( TF : PCA ) ( P=0.06 ) plasma levels and in TF : Ag cytofluorimetric expression on CD33 ( + ) cells ( P=0.04 ) . - pubmed
Arachidonic acid ( AA ) metabolites derived from both the lipoxygenase ( LOX ) and cyclooxygenase ( COX ) pathways transduce a variety of signals related to cell growth . - pubmed
The catalytic activity of PARP was inhibited by the potent isoindolinone - based PARP inhibitor INO-1001 or by PARP-1 genetic deficiency . - pubmed
The ability of the inhibitor to block the catalytic activity of PARP was confirmed by immunohistochemical detection of poly ( ADP - ribose ) , the product of the enzyme in the heart . - pubmed
Nectin - like molecule-1 / TSLL1 / SynCAM3 : a neural tissue - specific immunoglobulin - like cell - cell adhesion molecule localizing at non - junctional contact sites of presynaptic nerve terminals , axons and glia cell processes . - pubmed
Nectins are Ca2 + -independent immunoglobulin - like cell - cell adhesion molecules and comprise a family of four members . - pubmed
Five nectin - like molecules ( Necls ) which have domain structures similar to those of nectins have been identified and here we characterize Necl-1 / TSLL1 / SynCAM3 , from now on referred to as Necl-1 . - pubmed
Necl-1 showed Ca2 + -independent homophilic cell - cell adhesion activity . - pubmed
It furthermore showed Ca2 + -independent heterophilic cell - cell adhesion activity with Necl-2 / IGSF4 / RA175 / SgIGSF / TSLC1 / SynCAM1 from now on referred to as Necl-2 , nectin-1 and nectin-3 , but not with Necl-5 or nectin-2 . - pubmed
These results indicate that Necl-1 is a neural - tissue - specific Ca2 + -independent immunoglobulin - like cell - cell adhesion molecule which potentially has membrane - associated guanylate kinase subfamily member - binding activity and localizes at the non - junctional cell - cell contact sites . - pubmed
This defective IL-10 production could be explained by a reduction in the cAMP - dependent signalling pathway , which mediates IL-10 production . - pubmed
Fluorescence quenching experiments determined that purified FhuD bound hydroxamate - type siderophores . - pubmed
The role of Fhu in siderophore - iron transport was also characterized . - pubmed
A fhu mutant , ACFhu1 , was equally sensitive to the iron - dependent antibiotic streptonigrin as the wild - type strain , suggesting that ACFhu1 was not reduced for intracellular iron concentrations in the absence of exogenous siderophore . - pubmed
However , ACFhu1 transported significantly less siderophore - bound iron in ( 55 ) Fe accumulation assays . - pubmed
The small GTPase Rac cycles between the membrane and the cytosol as it is activated by nucleotide exchange factors ( GEFs ) and inactivated by GTPase - activating proteins ( GAPs ) . - pubmed
Solubility in the cytosol is conferred by binding of Rac to guanine - nucleotide dissociation inhibitors ( GDIs ) . - pubmed
Thus , the major route for dissociation is conversion of membrane - bound GTP - Rac to GDP - Rac ; however , dissociation of GTP - Rac occurs at a detectable rate . - pubmed
Overexpression of the GEF Tiam1 unexpectedly decreased k(off ) for wtRac , most likely by converting membrane - bound GDP - Rac back to GTP - Rac . - pubmed
We hereby present evidence that prolonged exposure to CO ( 2 ) impairs developmental retinal neovascularization through a mechanism involving increased endothelial nitric oxide synthase and induction of a nitrative stress ; effects of hypercapnia are independent of its hyperaemic effects . - pubmed
Moreover , we demonstrate that an in vivo nitrative stress associated with retinal vasoobliteration results in nitration of arachidonic acids into trans - arachidonic acids ( TAAs ) , which can act as mediators of nitrative stress by causing microvascular degeneration by inducing expression of the antiangiogenic factor thrombospondin-1 . - pubmed
Characterization and tissue - specific expression of two lepidopteran farnesyl diphosphate synthase homologs : implications for the biosynthesis of ethyl - substituted juvenile hormones . - pubmed
The biogenesis of these JHs requires the synthesis of ethyl - substituted farnesyl diphosphate ( FPP ) by FPP synthase ( FPPS ) . - pubmed
Type - I FPPSs display unique active site substitutions , including several in and near the first aspartate - rich motif , whereas type - II proteins have a more " conventional " catalytic cavity . - pubmed
Increased generation of oxygen - derived free radicals resulted in the Ras mutant cells accumulation of lipofuscin - like pigments during ageing . - pubmed
In contrast , the Ras mutant cells contained decreased levels of alpha tocopherol even in the young cells . - pubmed
Protein nitrotyrosine , a marker of the reactive nitrogen species , has significantly increased in the senescent Ras mutant cells . - pubmed
Overexpression of antiapoptosis protein Bcl-2 in AML cells was directly correlated with p53 expression and phosphorylation on serine residues 15 , 46 , and 392 . - pubmed
Use of integrated FDG PET / CT imaging in pulmonary carcinoid tumours . - pubmed
OBJECTIVE : To evaluate the usefulness of integrated FDG PET / CT in pulmonary carcinoid tumours . - pubmed
CONCLUSIONS : ( 18 ) F - fluorodeoxyglucose PET / CT imaging improves accurate localization of metabolic activity and thus the interpretation of pulmonary lesions on CT . - pubmed
In 37 % of patients , nonnucleoside reverse transcriptase inhibitor drug concentrations were still detectable 1 week after stopping . - pubmed
The type IIa Na (+)/ P ( i ) cotransporter ( NaPi - IIa ) plays a key role in the reabsorption of inorganic phosphate ( P ( i ) ) in the renal proximal tubule . - pubmed
Laboratory parameters measured were blood glucose , urinary ketones , electrolytes , urea creatinine , arterial blood gas ( ABG ) and infectious screen . - pubmed
The median time to normalize ABG was 19 hr ( 5.3 - 39 ) while the median time for the urinary ketones to disappear was 1day ( 1 - 3 ) . - pubmed
Pael receptor is involved in dopamine metabolism in the nigrostriatal system . - pubmed
We report here the X - ray structures of the periplasmic sensor domain of CitA in the citrate - free and citrate - bound states . - pubmed
A screen of candidate cell adhesion molecules revealed that the expression of connexin ( Cx )- 43 correlated with the age - dependent effect of cell contact in cell pair experiments . - pubmed
IMCL levels and skeletal muscle oxidative capacity were determined in vivo , using localized ( 1 ) H magnetic resonance spectroscopy ( MRS ) and dynamic ( 31 ) P MRS , respectively . - pubmed
strain GIN1 ) was previously isolated on the basis of its strong adherence to coal fly ash ( CFA ) and titanium dioxide particles from CFA sedimentation ponds of an electrical power plant in Israel . - pubmed
The interaction of the bacterium with oxides has been shown to be mediated by a cell surface protein designated TiBP ( titanium binding protein ) involving primarily strong , non - electrostatic forces . - pubmed
Vision - related adverse effects of commonly used medications , such as amiodarone or phosphodiesterase inhibitors , should be considered when evaluating vision problems . - pubmed
Root - end cavities in groups A and B were filled with MTA and NEC , respectively , and were then stored in 100 % humidity for 24 h. The samples of groups 1 and 2 were , respectively , immersed in normal saline ( NS ) and phosphate buffer saline solutions for 1 week . - pubmed
Contraction of both veins and arteries were significantly inhibited by the 5 - HT ( 2A ) receptor antagonist ketanserin . - pubmed
No effect on contraction was seen with the cyclooxygenase inhibitor indomethacin , the alpha ( 1 ) receptor antagonist prazosin or the angiotensin AT ( 1 ) receptor antagonist saralasin neither in veins nor arteries . - pubmed
We have identified the periplasmic lipoprotein HbpA , a protein hitherto implicated in heme acquisition , as the cognate PBP that specifically binds reduced ( GSH ) and oxidized glutathione ( GSSG ) forms of glutathione with physiologically relevant affinity , while it exhibits marginal binding to hemin . - pubmed
Dissection of the ligand preferences of HbpA showed that HbpA does not recognize bulky glutathione S conjugates or glutathione derivatives with C - terminal modifications , consistent with the need for selective import of useful forms of glutathione and the concomitant exclusion of potentially toxic glutathione adducts . - pubmed
In addition to several clinical variables , several biomarkers were significant predictors of death , including log - transformed concentrations of hemoglobin ( hazard ratio=0.77 ; P & lt ; 0.001 ) , soluble ST2 ( hazard ratio=1.38 ; P & lt ; 0.001 ) , and amino - terminal pro - B - type natriuretic peptide ( hazard ratio=1.19 ; P & lt ; 0.001 ) . - pubmed
Reaction with the substrate quantitatively affords a monolayer of electronically intact clusters doubly linked to the surface via Au - S bonds , as demonstrated by a combination of STM , XAS / XMCD and XPS studies . - pubmed
Our sequence analysis showed that B12 - dependent and B12 - independent glycerol dehydratases are not related , suggesting that they evolved from different ancestors . - pubmed
Arachidonoyl ethanolamide ( AEA)-induced apoptosis is mediated by J - series prostaglandins and is enhanced by fatty acid amide hydrolase ( FAAH ) blockade . - pubmed
A previous report from our laboratory showed that AEA induced cell death in a cyclooxygenase-2 ( COX-2)-dependent manner and in this report our data indicate that AEA - induced apoptosis is mediated by COX-2 metabolic products of the J - series . - pubmed
Using antagonists of cannabinoid receptor 1 , cannabinoid receptor 2 , or transient receptor potential cation channel , subfamily V , member 1 , it was observed that cannabinoid receptor inhibition did not block AEA - mediated cell death . - pubmed
In contrast , an inhibitor of fatty acid amide hydrolase ( FAAH ) potentiated AEA - induced J - series PG synthesis and apoptosis . - pubmed
These results suggest that the metabolism of AEA to J - series PGs regulates the induction of apoptosis in cells with elevated COX-2 levels . - pubmed
Our data further indicate that the proapoptotic activity of AEA can be enhanced by combining it with an inhibitor of FAAH . - pubmed
As such , AEA may be an effective agent to eliminate tumor cells that over - express COX-2 . - pubmed
The structures of these compounds were confirmed by a chemical analysis and spectrophotometric methods ( UV , 1H- , 13C - NMR , ESI - MS ) . - pubmed
No amino acid substitutions predicted to be functionally damaging were found in TLR7 . - pubmed
In the case of dioxin - like compounds ( DLCs ) , it has been hypothesized that single nucleotide polymorphisms ( SNPs ) in genes associated with aryl hydrocarbon receptor ( AHR)-regulated pathways may result in greater susceptibility to DLC toxicity . - pubmed
Confocal calcium imaging was used to detect spontaneous calcium release ( calcium sparks and waves ) from the SR . - pubmed
Inhibition of SR calcium uptake reduced the calcium transient ( F / F(0 ) ) from 1.77 + -0.17 to 1.12 + -0.18 ( p = 0.002 ) and abolished calcium sparks and waves . - pubmed
An N - linked glycosylation system encoded by the pgl gene cluster mediates the synthesis of a rigidly conserved heptasaccharide that is attached to protein substrates or released as free oligosaccharide in the periplasm . - pubmed
coli pEtN transferases can mediate the addition of pEtN to N - glycans . - pubmed
The emission shows a decrease in intensity with an increase in external temperature , however contrary to the normal behavior of Ho ( 3 + ) , no significant change in the FIR ( fluorescence intensity ratio ) of ( 5)F(4 ) ( 5)I(8 ) and ( 5 ) S ( 2 ) ( 5 ) I ( 8 ) transitions is noted in the present host . - pubmed
CONCLUSIONS : These data suggest an important role for CD44 in the context of tamoxifen - resistance where it may augment cellular response to erbB ligands and HA , factors that are reported to be present within the tumour microenvironment in vivo . - pubmed
Epac2 mediates cardiac beta1 - adrenergic - dependent sarcoplasmic reticulum Ca2 + leak and arrhythmia . - pubmed
BACKGROUND : beta - Adrenergic receptor ( beta - AR ) activation can provoke cardiac arrhythmias mediated by cAMP - dependent alterations of Ca ( 2 + ) signaling . - pubmed
However , cAMP can activate both protein kinase A and an exchange protein directly activated by cAMP ( Epac ) , but their functional interaction is unclear . - pubmed
In heart , selective Epac activation can induce potentially arrhythmogenic sarcoplasmic reticulum ( SR ) Ca ( 2 + ) release that involves Ca ( 2 +)/ calmodulin - dependent protein kinase II ( CaMKII ) effects on the ryanodine receptor ( RyR ) . - pubmed
METHODS AND RESULTS : We tested whether physiological beta - AR activation causes Epac - mediated SR Ca ( 2 + ) leak and arrhythmias and whether it requires Epac1 versus Epac2 , beta ( 1 )- AR versus beta ( 2 )- AR , and CaMKIIdelta - dependent phosphorylation of RyR2 - S2814 . - pubmed
beta - AR activation with protein kinase A inhibition mimicked 8 - CPT effects on SR Ca ( 2 + ) leak and was prevented by blockade of beta ( 1 )- AR but not beta ( 2 )- AR . - pubmed
CaMKII inhibition ( KN93 ) and genetic ablation of either CaMKIIdelta or CaMKII phosphorylation on RyR2 - S2814 prevented 8 - CPT - induced SR Ca ( 2 + ) leak . - pubmed
CONCLUSIONS : beta ( 1 )- AR activates Epac2 to induce SR Ca ( 2 + ) leak via CaMKIIdelta - dependent phosphorylation of RyR2 - S2814 . - pubmed
Impaired glycolytic metabolism causes chondrocyte hypertrophy - like changes via promotion of phospho - Smad1 / 5 / 8 translocation into nucleus . - pubmed
Interestingly , combinational treatment with NaF and lactate enhanced translocation of phospho - Smad1 / 5 / 8 to the nucleus , as well as gene expression of ALP , VEGF , COL10a1 , and matrix metalloproteinase13 ( MMP13 ) , which were the markers of late mature and hypertrophic chondrocytes . - pubmed
CONCLUSIONS : These findings suggest that decreased ATP production by NaF promotes hypertrophy - like changes via activation of phospho - Smad1 / 5 / 8 in the presence of lactate . - pubmed
Azithromycin reduces and prevents broncholaveolar lavage ( BAL ) neutrophilia after lung transplantation ( LTx ) . - pubmed
Azithromycin reduced both % BAL neutrophilia and IL-17 + cells ( both p = 0.016 ) in the LB group . - pubmed
Moreover , azithromycin significantly decreased the number of IL-17 + cells in the airway wall , which may further explain its effect on BAL neutrophilia . - pubmed
Over - activation of microglia cells in the brain contributes to neurodegenerative processes promoted by the production of various neurotoxic factors including pro - inflammatory cytokines and nitric oxide . - pubmed
Furthermore , mitochondrial localization of dynamin - related protein 1 ( Drp1 ) ( a key regulator of mitochondrial fission ) was increased and accompanied by de - phosphorylation of Ser637 in Drp1 . - pubmed
Interestingly , inhibition of LPS - induced mitochondrial fission and reactive oxygen species ( ROS ) generation by Mdivi-1 and Drp1 knock - down attenuated the production of pro - inflammatory mediators via reduced nuclear factor kappa - light - chain - enhancer of activated B cells ( NF - kappaB ) and mitogen - activated protein kinase ( MAPK ) signaling . - pubmed
Likewise , ERK phosphorylation was markedly enhanced in PAF - stimulated VSMCs , and this was attenuated by WEB2086 , but not by EGF receptor inhibitor , demonstrating the specificity of PAF receptor ( PAFR ) in PAF - induced ERK phosphorylation . - pubmed
In addition , PAF - induced ERK phosphorylation and MMP-2 production were significantly attenuated by beta - arrestin2 depletion . - pubmed
Taken together , the study shows that PAF enhances MMP-2 production in VSMCs via a beta - arrestin2 - dependent ERK signaling pathway . - pubmed
We assessed the temporal profile of alanine aminotransferase ( ALT ) , bilirubin ( Bil ) , and alkaline phosphatase ( ALP ) . - pubmed
Sox2 promotes tamoxifen resistance in breast cancer cells . - pubmed
Here , we examined their role in the development of resistance to the oestrogen receptor antagonist tamoxifen . - pubmed
Tamoxifen - resistant cells were enriched for stem / progenitors and expressed high levels of the stem cell marker Sox2 . - pubmed
Silencing of the SOX2 gene reduced the size of the stem / progenitor cell population and restored sensitivity to tamoxifen . - pubmed
Conversely , ectopic expression of Sox2 reduced tamoxifen sensitivity in vitro and in vivo . - pubmed
Gene expression profiling revealed activation of the Wnt signalling pathway in Sox2 - expressing cells , and inhibition of Wnt signalling sensitized resistant cells to tamoxifen . - pubmed
Together , these results suggest that development of tamoxifen resistance is driven by Sox2 - dependent activation of Wnt signalling in cancer stem / progenitor cells . - pubmed
However , some cancer cells lack asparagine synthetase ( ASNS ) , the key enzyme for asparagine synthesis . - pubmed
Asparaginase ( ASNase ) contributes to eradication of acute leukemia by decreasing asparagine levels in serum and cerebrospinal fluid . - pubmed
ASNase cotreatment significantly enhanced gemcitabine or etoposide cytotoxicity against glioblastoma cells . - pubmed
However , combinatorial therapy with ASNase and temozolomide resulted in significant growth suppression for an extended duration of time . - pubmed
Bis - styrylbenzenes have been previously reported as potential Abeta imaging agents . - pubmed
To further explore their potency as ( 19 ) F MRI contrast agents we synthetized several novel fluorinated bis - styrylbenzenes and studied their fluorescent properties and amyloid - beta binding characteristics . - pubmed
Unfortunately initial post - mortem ( 19 ) F NMR studies showed that these compounds as yet do not warrant further MRI studies due to the reduction of the ( 19 ) F signal in the environment of the brain . - pubmed
Twenty pairs of Zn - contaminated soils ( contaminated and reference soils ) were collected on the field , and their enzymatic FS ( arylsulfatase , protease , phosphatase and urease ) and metal fractions ( total and bioavailable ) were analyzed . - pubmed
New RSSI - based and existing FS scores were calculated for each enzyme and correlated to the Zn fractions . - pubmed
One of the new RSSI - based scores was well correlated with the bioavailable labile Zn concentration for the arylsulfatase , phosphatase and urease ( coefficients of regression higher than 0.50 ) . - pubmed
Other FS scores were correlated to labile Zn for only one enzyme , which varied according to the score . - pubmed
The new RSSI - based score thus better attributed Zn toxicity to field - collected soils than other FS scores . - pubmed
Alanine substitution of the conserved residue H98 in prM disrupts the switch by inhibiting dissociation of M from E at low pH , resulting in impaired prM processing and decreased virus infectivity . - pubmed
Selenadiazole derivatives as potent thioredoxin reductase inhibitors that enhance the radiosensitivity of cancer cells . - pubmed
The redox enzyme thioredoxin reductase ( TrxR ) plays a vital role in restoring cellular thiol redox balance disrupted by radiation - induced reactive oxygens species ( ROS ) generation and oxidative damage . - pubmed
In this study , a series of 1,2,5 - selenadiazoles have been synthesized and identified as highly effective inhibitors of TrxR to disrupt the intracellular redox balance , and thus significantly enhanced the sensitivity of cancer cells to X - ray . - pubmed
Interestingly , we also found the interaction sites between 1,2,5 - selenadiazole and the model peptide of TrxR , which can be confirmed by MALDI - ToF - MS . - pubmed
These results clearly demonstrate TrxR as a potential target for therapy of radioresistant cancers , and selenadiazole derivatives may be attractive radiosensitizing agent by targeting TrxR. - pubmed
AIM : Oral contraceptive use influences the risk for certain cancers . - pubmed
We discuss the current understanding of IPF and BPF disease development , from the contribution of alveolar epithelial cells and inflammation to the role of fibroblasts and cytokines , and draw conclusions about what we have learned from the intratracheal bleomycin model of lung fibrosis . - pubmed
It was seen that CDHA coatings remarkably reduced the mass loss of AZ31 alloy after 7days of immersion in SBF . - pubmed
Mutagenesis of the cysteine and glutamate residues in the N - terminal metal - binding domain ( N - MBD ) impaired the function of TaHMA2 . - pubmed
These data suggest that cysteine and glutamate residues are important for the metal - binding / translocation function of TaHMA2 . - pubmed
The GO was coated with hydrophilic branched polyethyleneimine ( BPEI ) , while Concanavalin A ( Con A ) as a model lectin protein was employed to fabricate the functionalized composites to evaluate the feasibility of this strategy . - pubmed
Cell Polarity Kinase MST4 Cooperates with cAMP - dependent Kinase to Orchestrate Histamine - stimulated Acid Secretion in Gastric Parietal Cells . - pubmed
A coupling protein is ezrin , whose phosphorylation at Ser-66 by PKA is required for parietal cell activation . - pubmed
Here we show that PKA cooperates with MST4 to orchestrate histamine - elicited acid secretion by phosphorylating ezrin at Ser-66 and Thr-567 . - pubmed
Histamine stimulation activates PKA , which phosphorylates MST4 at Thr-178 and then promotes MST4 kinase activity . - pubmed
Importantly , MST4 is important for acid secretion in parietal cells because either suppression of MST4 or overexpression of non - phosphorylatable MST4 prevents the apical membrane reorganization and proton pump translocation elicited by histamine stimulation . - pubmed
Ribavirin alone , or in combination with interferon , has been the mainstream therapy for HCV infection after transplantation . - pubmed
Finally , the effect of piperine on Na (+ ) and Ca ( 2 + ) channels were evaluated using the whole cell patch clamp techniqueOur results revealed that piperine decreased mortality in the MES - induced seizure model . - pubmed
Finally , whole cell patch clamp analysis suggested an inhibitory effect of piperine on Na (+ ) channels . - pubmed
Together , our data suggest Na (+ ) channel antagonist activity as a contributor to the complex anticonvulsant mechanisms of piperine . - pubmed
We identified a series of conserved amino acid residues and motifs that are likely to play a major role for the function of MLO proteins . - pubmed
Possibly , four conserved water molecules stabilize oxyanion hole in unliganded state , but they vacate these positions when the enzyme ( hGMPS)-substrate complex is formed . - pubmed
The phase of oscillation in per expression was found to commence 3.5h earlier under WC 25.5:24.5 C as compared to WC 29:21 C. This difference was in close agreement with the eclosion time difference between the two temperature cycles , suggesting that the mechanism that responds to the temperature amplitude involves the circadian clock . - pubmed
The present work considers the possibility of doing the same by using the basic C2 units of carbo - meric architectures . - pubmed
The C2 units are shown to stabilize the singlet biradical by spin delocalization , two of them playing approximately the same role as one radical - insulating 1,4 phenylene moiety . - pubmed
Identification of Toxoplasma TgPH1 , a pleckstrin homology domain - containing protein that binds to the phosphoinositide PI ( 3,5 ) P2 . - pubmed
Here , by using a phosphoinositide pull - down assay , we identified TgPH1 in Toxoplasma a protein conserved in many apicomplexan parasites . - pubmed
TgPH1 binds specifically to PI ( 3,5 ) P2 , shows punctate intracellular localization , but plays no vital role for tachyzoite growth in vitro . - pubmed
Therefore , our study reveals an unusual feature of TgPH1 which binds preferentially to PI ( 3,5 ) P2 . - pubmed
Rapa was released from the PLGA nanospheres incorporating rapamycin ( Rapa - PLGA - NS ) with time while the Rapa - PLGA - NS were hydrolytically degraded . - pubmed
The microtubule - associated protein 1 light chain ( LC3 ) of an autophagy marker was significantly high for the Rapa - PLGA - NS group compared with the free Rapa group even after four days incubation . - pubmed
RESULTS : Diet - tissue isotope enrichment ( trophic enrichment factor , TEF ) values between dietary herring and seal tissues for carbon ( Delta13 C ) were +0.7 % for red blood cells , +1.9 % for hair and +1.1 % for muscle . - pubmed
An HRAM LC - MS method was validated for quantitative analysis of 26 steroid metabolites in hydrolyzed urine samples . - pubmed
CONCLUSIONS : Our HRAM LC - MS assay successfully quantifies 26 steroids in urine . - pubmed
Further investigation revealed that HDAC1 , a histone deacetylase that was abnormally overexpressed in hepatocellular carcinoma , could bind to the miR-195 promoter via Smad3 and cause hypoacetylation in the histones associated with the miR-195 promoter in hepatoma cells . - pubmed
BACKGROUND : Nutritional choices , which include the source of dietary fatty acids ( FA ) , have an important significant impact on coronary artery disease ( CAD ) . - pubmed
On the other hand , catalase activity , conjugated dienes and malondialdehyde were increased . - pubmed
Furthermore , divergent associations of these Trans FA accumulations were observed with low - density lipoprotein - cholesterol/ high - density lipoprotein - cholesterol ( LDL - C / HDL - C ) ratio , Apolipoprotein B ( ApoB ) , lipid peroxidation parameters , high - sensitivity C Reactive Protein ( hs - CRP ) , Interleukin 6 ( IL-6 ) , tumor necrosis factor alpha ( TNF - alpha ) and Gensini score . - pubmed
We aimed to evaluate whether pretransplant aortic arch calcification ( AoAC ) can predict post - transplant cardiovascular or cerebrovascular events ( CVEs ) , and to assess its association with post - transplant plasma calcium levels and renal function in one - year follow - up . - pubmed
Zhang and colleagues ' study sheds light on the development of magnesium pills or calcitonin gene - related peptide - delivery system for the prevention or treatment of osteoporosis . - pubmed
Ag accumulation and bacterial infection in PAT demonstrate that PAT not only provides energy and regulatory factors , but can also directly participate in immune protection , indicating a new immune function of PAT for host immunity . - pubmed
Ombitasvir / paritaprevir / ritonavir + dasabuvir + ribavirin in HCV genotype 1 infected patients who failed previous protease inhibitor therapy . - pubmed
Accordingly , PARP3 interacts with and ADP - ribosylates GSK3beta , a positive regulator of Rictor ubiquitination and degradation . - pubmed
Here we show that Takinib , a highly discriminatory inhibitor of transforming growth factor Beta - activated kinase 1 ( TAK1 ) , selectively and potently reduces TNF production in pro - inflammatory THP-1 macrophages . - pubmed
A complete survey of 110 cytokines , showed robust loss of proinflammatory cytokine responsiveness to lipopolysaccharide ( LPS ) and interferon gamma ( IFNgamma ) challenge in response to Takinib . - pubmed
The mechanisms of action of Takinib was recapitulated in TAK1 KO macrophages . - pubmed
Furthermore , Takinib blocked the ability of exogenously added LPS to promote phosphorylation of , c - Jun , p38 protein kinases as well as downstream transcription factors regulated by nuclear factor kappa - light - chain - enhancer of activated B cells ( NFkappaB ) . - pubmed
Our findings demonstrate that Takinib has utility in the treatment inflammatory disease by locally suppressing TNF production from invading macrophages . - pubmed
The group treated with meloxicam presented the highest rise in the GPx level ( p = 0.052 ) . - pubmed
Histone demethylase KDM6B regulates 1,25 - dihydroxyvitamin D3 - induced senescence in glioma cells . - pubmed
In this study , we show that 1,25 - dihydroxyvitamin D3 ( the active form of vitamin D3 ) induces senescence of glioma cells and increases the expression of senescence markers , INK4A and cyclin - dependent kinase inhibitor 1A ( CDKN1A ) . - pubmed
1,25 - Dihydroxyvitamin D3 also upregulates the expression of histone demethylase , KDM6B. - pubmed
Knockdown of KDM6B attenuates 1,25 - dihydroxyvitamin D3 - induced senescence and upregulation of INK4A and CDKN1A. - pubmed
PURPOSE OF REVIEW : This review outlines recent discoveries on the crosstalk between oxygen metabolism and iron homeostasis , focusing on the role of HIF-2 ( hypoxia inducible factor-2 ) in the regulation of iron metabolism under physiopathological conditions . - pubmed
RECENT FINDINGS : The importance of the hepcidin / ferroportin axis in the modulation of intestinal HIF-2 to regulate iron absorption has been recently highlighted . - pubmed
Latest advances also reveal a direct titration of the bone morphogenetic proteins by the erythroferrone contributing to liver hepcidin suppression to increase iron availability . - pubmed
Materials and methods : The sinus defects were grafted with rhBMP-2+ACS ( Group A ) , rhBMP-2 - coated TCP ( Group B ) , and platelet - rich fibrin - mixed TCP ( Group C ) . - pubmed
On the converse , stimuli that suppress growth such as testosterone depletion or acute aerobic exercise increased Arrdc2 and Arrdc3 expression in skeletal muscle . - pubmed
These data supplement important technical and biological information on immune function of Rap2a in macrophages activated by LPS , recently reported by us ( Carvalho et al . - pubmed
In sum , we report alteration of UNC45A localization in the setting of chemotherapeutic treatment of cells with paclitaxel , and localization of UNC45A to MTs both in vitro and in vivo . - pubmed
The opioid epidemic currently plaguing the United States has been exacerbated by an alarming rise in fatal overdoses as a result of the proliferated abuse of synthetic mu opioid receptor ( MOR ) agonists , such as fentanyl and its related analogues . - pubmed
The study discusses thermodynamic parameters , including changes in Gibbs free energy , entropy , and enthalpy , for the adsorption of Ni2 + and Pb2 + on DSP , and revealed that the adsorption process was spontaneous and exothermic under natural conditions . - pubmed
The maximum Ni2 + and Pb2 + adsorption capacities of DSP were 33.02 and 36.50 mg / g , respectively . - pubmed
The primary result was the percentage of bone - to - implant contact ( BIC ) around the titanium implants with or without a strontium - coated surface . - pubmed
CONCLUSION : Nine of 11 studies reported that titanium implants coated with strontium showed significantly higher BIC ( P & lt ; .001 to P & lt ; .05 ) . - pubmed
A bio - inspired , ATP - driven nucleation growth assembly is demonstrated using an amphiphilic naphthalene diimide ( NDI ) derivative appended with guanidinium receptors to promote specific salt - bridge type interaction with nucleotide phosphates . - pubmed
Furthermore , overexpression of a phospho - mimetic S175E AMOT mutant , which has been established to enhance AMOT - YAP binding , increases beta - catenin activity and rescues neurogenesis on stiff substrates . - pubmed
SP - D showed a negative correlation with percutaneous oxygen saturation and positive correlations with serum lactate dehydrogenase , Krebs von den Lungen ( KL)-6 , and percentage of lymphocytes in bronchoalveolar lavage ( BAL ) . - pubmed
According to the World Health Organization , there will be a return to the pre - penicillin era by 2050 if no new antimicrobials are discovered . - pubmed
As such , bilirubin may be marker for elevated risk of poor post - operative outcomes and should be more frequently measured after cardiac surgery . - pubmed
Some clinical investigations demonstrated that rifampin pretreatment decreased exposure of OATP1B substrates , while other studies found no differences , and the potential involvement of other mechanisms in these observed DDIs can not be definitively ruled out . - pubmed
Gt1 could only function to maintain zinc homeostasis in glycerol , while the block of Gt1 function might lead to Zrt1 upregulation in glucose . - pubmed
CONCLUSIONS : The zinc transport capabilities of Gt1 and Zrt1 were identified in vivo and in vitro . - pubmed
As a crucial regulator of ABA signaling , ABSCISIC ACID - INSENSITIVE5 ( ABI5 ) is involved in many aspects of plant growth and development . - pubmed
Nevertheless , whether ABI5 regulates anthocyanin biosynthesis has not been reported . - pubmed
A series of biochemical tests showed that MdABI5 specifically interacts with MdbHLH3 , a positive regulator of anthocyanin biosynthesis . - pubmed
Moreover , MdABI5 acted as an enhancer in the ABA - promoted interaction between MdMYB1 and MdbHLH3 . - pubmed
Finally , antisense suppression of MdbHLH3 significantly reduced anthocyanin biosynthesis promoted by MdABI5 , indicating that MdABI5 - promoted anthocyanin biosynthesis was dependent on MdbHLH3 . - pubmed
Taken together , our data suggest that MdABI5 plays a positive role in ABA - induced anthocyanin biosynthesis by modulating the MdbHLH3 - MdMYB1 complex . - pubmed
Our work enriches the functional studies of ABI5 and further broadens the regulatory network of ABA - mediated anthocyanin biosynthesis , providing new insights for further study of the transcriptional regulatory mechanisms of anthocyanin biosynthesis . - pubmed
We assessed the number of filled prescriptions of inhaled corticosteroids ( ICS ) , ICS / long - acting beta2 agonists ( LABA ) , and short - acting beta2 agonists ( SABA ) , as well as the number of days ' supply of oral corticosteroid ( OCS ) from the 20th week of pregnancy until delivery . - pubmed
Astrocytes release molecules such as thrombospondin , hevin , secreted protein acidic rich in cysteine , etc . - pubmed
After bilateral ureteral ligation , serum granulocyte - macrophage colony - stimulating factor levels were increased and in vitro tritiated thymidine suicide studies showed an increased proliferative rate of the CFU - c. Since azathioprine is a predominantly cell cycle - specific agent , we suggest that increased sensitivity to azathioprine is related to the increased proliferative rate of the CFU - c. The findings provide a rationale for a clinical policy of azathioprine reduction when there is depressed renal function . - pubmed
At 10(-7 ) M , Iso produced approximately maximal responses at all ages in the ECH but elicited only minimal responses at all ages in the ERH , approximately ten times this concentration being required to produce maximal responses in the ERH . - pubmed
Thyroidectomy and thyroxine - injection markedly altered fibrinogen metabolism : thyroid hormone accelerated fibrinogen catabolism but also stimulated synthesis . - pubmed
Further observations on the effects of S - triazine derivatives on purine metabolizing enzymes . - pubmed
Elution volume from a Sephadex LH-20 column , development in three ascending paper chromatographic ( APC ) systems , acetylation and development in a fourth APC system followed by gas liquid chromatography ( GLC ) of the acetylated products tentatively identified the radioactive - metabolites as 5alpha - androstan-17beta - ol-3 - one ( DHT ) , 5alpha - androstan-3alpha,17beta - diol ( 3alphaol ) and 5alpha - androstan-3beta,17beta - diol ( 3beta - ol ) . - pubmed
At 40 degrees , pH 8.0 , and in the presence of 10 mM Ca2 + , V was found to be about 4 X 10(3 ) mumol min(-1 ) mg of protein(-1 ) . - pubmed
Each component was inactive by itself , but cobaltochelatase activity was reconstituted upon mixing CobN and CobST . - pubmed
Spectroscopic data revealed that the reaction product was cob ( II ) yrinic acid a , c - diamide , and experiments with a coupled - enzyme incubation system containing both cobaltochelatase and cob ( II ) yrinic acid a , c - diamide reductase ( F. Blanche , L. Maton , L. Debussche , and D. Thibaut , J. Bacteriol . - pubmed
She was initially treated with cytokines plus 5 - fluorouracil derivative , resulting in a complete remission lasting over a year . - pubmed
Values for the fractional excretion of glucose were low and consistent , with or without angiotensin II . - pubmed
Although the unbound fraction ( fu ) of chlorothiazide was unchanged between treatments , the renal ( CLr ) and the secretion clearances were reduced by about 50 % in the presence of angiotensin II ; the excretion ratio [ ER = CLr/(fu . - pubmed
Transport parameters ( + /- SD ) were obtained and the corrected intrinsic secretory clearance [ ( Vmax / GFR)/Km ] of chlorothiazide was 123 + /- 18 without angiotensin II vs. 72.8 + /- 30.0 with angiotensin II . - pubmed
From the study of absorption and fluorescence spectra of the complex " Hoechst-33258"--DNA at different pH it is shown that AT -- specific complex with DNA is formed by the neutral dye molecule , whereas the cationic state of the dye molecule forms the nonspecific complex . - pubmed
A secondary - structure comparison between the nucleotide - binding domain of NAD(+)-dependent enzymes and that of NADP (+)- dependent enzymes also suggests that Asp38 could play an important role in cofactor specificity . - pubmed
The steroid receptor level was assayed by hormone binding techniques and specific mRNAs analyzed by solution hybridization using 35S - labelled RNA probes complementary to the ligand - binding domain of the ER receptor gene and a 160 bp PanI - Pvul fragment of the IGF - I gene . - pubmed
One hour after a single injection of oestradiol ( 1 micrograms / kg BW ) , the nuclear oestrogen receptor ( ERn ) mean level was increased 3 - fold whereas the ER mRNA content had not changed significantly . - pubmed
Cytochrome P450IA1 mRNA was induced in colon following a single oral administration of I3C or 5,6BF . - pubmed
Both inducers caused an approximately 2 - fold increase in P450IA1 mRNA at 4 h and a 10 - fold increase at 16 h. In contrast , both cytochrome P450IA1 and IA2 mRNAs was increased over the control between 4 and 24 h. The total amount of P450IA mRNAs in liver at 4 and 16 h was increased about 2- and 4 - fold respectively by I3C ; 5,6BF induced the P450IA mRNAs 4- and 5 - fold respectively . - pubmed
1 -( 5 - Isoquinolinesulfonyl )- 2 - methylpiperazine , a putative inhibitor of protein kinase C , does not modify the respiratory response to SP . - pubmed
Rostrally , the heavily labeled enkephalin areas coincide with areas strongly immunostained for calcium - binding protein and with lightly stained areas in the dopamine and substance P immunoreactivity patterns . - pubmed
In the same region lightly stained areas in the enkephalin distribution match heavily labeled substance P areas . - pubmed
The moderately labeled enkephalin areas caudomedially in the nucleus accumbens are in register with the heavily labeled regions in the distribution of substance P and with the extremely heavily labeled regions in the distribution of dopamine . - pubmed
Altered ATP function of a vesicular stomatitis virus mutant detected by kinetic analysis of the transcriptase using phosphorylated ribavirin . - pubmed
D2 agonists lowered the binding of [ 3H ] raclopride to D2 receptors in striatal and anterior pituitary tissues . - pubmed
Pretreating the tissue with the D1 - selective antagonist SCH 23390 prevented the agonist - induced decrease in [ 3H ] raclopride binding to D2 sites in the striatum but not in the anterior pituitary , which has no D1 receptors . - pubmed
Conversely , a dopamine - induced reduction in the binding of [ 3H ] SCH 23390 to D1 receptors could be prevented by the D2 - selective antagonist eticlopride . - pubmed
The blocking effect by SCH 23390 was similar to that produced by a nonhydrolyzable guanine nucleotide analogue , and SCH 23390 reduced the number of agonist - labeled D2 receptors in the high - affinity state . - pubmed
The effect of SK&F 94482 ( BMY-25368 ) on 24 - hour intragastric acidity and plasma gastrin concentration in healthy subjects . - pubmed
There was also a sustained and significant 80 % rise in median 24 - h integrated plasma - gastrin concentration during dosing with SK&F 94482 ( 364 pmol h / L ) when compared with placebo ( 202 pmol h / L ; P = 0.003 ) . - pubmed
The HAPE fluids also contained detectable amounts of leukotriene B4 and other lipoxygenase products of arachidonic acid metabolism , complement fragments ( C5a ) , inhibitors of neutrophil chemotaxis , and acid proteases but not hydroxyproline , a constituent of collagen . - pubmed
It had reduced electrophoretic mobility , reacted normally with specific albumin antiserum and , in contrast to normal albumin , did not bind nickel . - pubmed
Sequence analysis showed that the minor band contained components with Ala - His - Lys- and His - Lys- N - terminal sequences , indicating removal of Asp and Asp - Ala respectively from the parent albumin which was found to have the expected N - terminal sequence of Asp - Ala - His - Lys . - pubmed
Both normal albumin and the minor component had an average of 0.32 residues of glucose bound as fructosamine . - pubmed
The role of arginyl residues in porphyrin binding to ferrochelatase . - pubmed
The data obtained show that modification of arginyl , but not lysyl , residues results in the rapid inactivation of ferrochelatase . - pubmed
Reaction with radiolabeled phenylglyoxal shows that modification of 1 arginyl residue causes maximum inhibition of enzyme activity . - pubmed
Comparison of the enzyme kinetics for modified versus unmodified enzyme show that modification with camphorquinone-10 - sulfonic acid has no effect on the Km for iron but does alter the Km for porphyrin . - pubmed
MPRP contains 115 amino acid residues including phosphorylated serine at residues 1 , 2 , 6 , 12 , and 15 , and 6 O - glycosidic carbohydrate units at residues 69 , 75 , 87 ( threonine ) and 96 , 103 , and 106 ( serine ) . - pubmed
MPRP has 5 phosphoserines , PRPs have 2 . - pubmed
MPRP inhibits the spontaneous precipitation ( primary precipitation ) of calcium phosphate salts from supersaturated solutions in addition to inhibiting seeded crystal growth ( secondary precipitation ) ( Oppenheim , F. G. , Offner , G. D. , and Troxler , R. F. ( 1982 ) J. Biol . - pubmed
Synthesis of a copolymer containing adenylic and deoxyadenylic acid residues with polynucleotide phosphorylase . - pubmed
ALA - dehydratase activation by zinc . - pubmed
The responses to 2 - chloroadenosine were affected to a lesser extent than were those to the other purines.6 Adenosine transport inhibitors , dipyridamole and dilazep , potentiated responses to adenosine , did not affect those to NCPCA , NECA , L - PIA and D - PIA but significantly reduced the responses to high doses of 2 - chloroadenosine.7 Relaxations evoked by 9 - beta - D - xylofuranosyladenosine which can activate intracellular but not extracellular adenosine receptors , were attenuated by dipyridamole but unaffected by 8 - phenyltheophylline.8 The results support the existence of an extracellular A ( 2 )/ R ( a ) subtype of adenosine receptor and an intracellular purine - sensitive site , both of which mediate relaxation . - pubmed
Concentrations of 0.5 mM acetaldehyde and 0.1 U / ml xanthine oxidase caused reversible dilations , whereas higher concentrations produced initial constrictions followed by reversible dilations . - pubmed
The following free radical scavengers inhibited the dilation when added to the lower concentrations of reactants : superoxide dismutase , a superoxide scavenger ; catalase , an H2O2 scavenger ; and mannitol , a hydroxyl scavenger . - pubmed
The data support the hypothesis that hydroxyl radicals can dilate pial arterioles , since all the scavengers can ultimately reduce levels of hydroxyl generated by acetaldehyde plus xanthine oxidase . - pubmed
Among the neurotransmitter bindings tested , only those of GABA and benzodiazepine in the cerebellum and beta - adrenergic ligands in the cerebral cortex are enhanced upon stimulation of phospholipid methyltransferase activity . - pubmed
Subsequent reduction of arterial pressure was observed with prazosin hydrochloride , an alpha - receptor antagonist . - pubmed
In the presence of vascular beta 2 - receptor blockade by propranolol , the hypertensive action of epinephrine was mediated by vascular alpha - receptors . - pubmed
Experiments that employed labeled FSH , reduced exposure time to purified FSH , or decreased calcium concentration in the medium proved that the increased FSH secretory rate was not due to contamination of the medium with the purified FSH used to stimulate the pituitary . - pubmed
Estradiol-17 beta concentrations were always greater on the day of the coitus - induced ovulatory LH surge ( mean = 51 + /- 5 pg / ml ) as compared to the previous day ( mean = 34 + /- 4 pg / ml ) when coitus did not result in a significant LH release . - pubmed
These data suggest that the ability to release LH in response to coitus is in part a function of the duration of exposure to estrogen by the hypothalamus and/or anterior pituitary . - pubmed
Gel electrophoresis analysis indicates there is at least one intrachain disulfide bond in beta - dystroglycan . - pubmed
Three - dimensional solution structure of the calcium channel antagonist omega - agatoxin IVA : consensus molecular folding of calcium channel blockers . - pubmed
The three - dimensional solution structure of omega - agatoxin IVA , which is a specific blocker of the P - type calcium channel isolated from funnel web spider venom and has a molecular mass of 5.2 kDa , was determined by two dimensional 1H NMR spectroscopy , combined with simulated annealing calculations . - pubmed
We report a patient who developed Philadelphia chromosome negative acute myeloblastic leukaemia with trisomy 8 and trisomy 11 after receiving treatment with alkylating agents and interferon for chronic myelocytic leukaemia positive for Philadelphia chromosome . - pubmed
We conclude that the leukaemic cells negative for Philadelphia chromosome may have developed as a result of treatment with alkylating agents and interferon in the present case . - pubmed
Reduced dose PVB treatment of stage IIC - IV testicular seminoma . - pubmed
Despite the information on the possible cancer hazard among SS - welders , current epidemiological data on cancer incidence and mortality do not offer clear evidence to determine whether exposure to nickel or CrVI compounds constitutes the more important risk factor for lung cancer . - pubmed
2 - and 4 - fluorinated 16 alpha (-)[ 125I ] iodoestradiol derivatives : synthesis and effect on estrogen receptor binding and receptor - mediated target tissue uptake . - pubmed
The effect of 2 - and 4 - fluoro substitution on the estrogen receptor - mediated tissue localization of radioiodinated 16 alpha - iodoestradiol ( 16 alpha - IE2 ) and its 11 beta - methoxy analogue ( 11 beta - OMe-16 alpha - IE2 ) was evaluated . - pubmed
The 4 - F analogue exhibited higher affinity for estrogen receptors than the corresponding 2 - F analogue , and these differences were more pronounced at higher incubation temperatures . - pubmed
Data in six components ( bile acids , esterified cholesterol in whole plasma , and free cholesterol in blood cells , bile , alpha lipoproteins , and beta lipoproteins ) were modeled simultaneously with the SAAM program . - pubmed
The analysis revealed extensive exchange of free cholesterol between HDL and liver , blood cells , and other tissues . - pubmed
There was net free cholesterol transport from HDL to the liver in most subjects . - pubmed
A large portion ( 4,211 mumol ) of total hepatic cholesterol comprised a pool that turned over rapidly ( t1/2 of 72 min ) by exchanging mainly with plasma HDL and was the major source of bile acids and biliary cholesterol . - pubmed
HDL plays a central role in free cholesterol exchange / transport between all tissues and plasma . - pubmed
As well , the relative potency of salicylic acid ( SA ) as compared with ASA for these reproductive effects has not been well investigated . - pubmed
The data failed to demonstrate a substantial potency difference between ASA and SA but some differences in toxicity were observed . - pubmed
Both the highest administered dose of SA and ASA treatment contributed to increased maternal peripartum death . - pubmed
Lungs treated immediately before reperfusion with catalase ( 5,000 units ) , desferrioxamine ( 2 mg / kg ) , NG - nitro - L - arginine methyl ester ( 5 mmol / l ) or saline showed a significant augmentation in pre - ischaemia - reperfusion hypoxic pulmonary vasoconstriction ( 57.7 + /- 6.0 % , 82.7 + /- 28.8 % , 95.2 + /- 36.6 % and 45.95 + /- 10.53 % respectively ) , an increase in albumin flux ( 0.35 + /- 0.04 , 0.31 + /- 0.06 , 0.29 + /- 0.04 and 0.33 + /- 0.02 ) and an increase in pre - ischaemia - reperfusion palmitic : linoleic acid ratio ( 0.64 + /- 0.08 , 0.51 + /- 0.19 , 0.5 + /- 0.04 and 0.17 + /- 0.07 ) . - pubmed
administered immediately before reperfusion prevented completely the changes in hypoxic pulmonary vasoconstriction ( -0.3 + /- 5.4 % ) , albumin flux ( 0.09 + /- 0.11 ) and palmitic : linoleic acid ratio ( -0.06 + /- 0.12 ) . - pubmed
In control lungs ( 2h of continuous perfusion ) , superoxide dismutase , catalase , desferrioxamine and saline did not affect hypoxic pulmonary vasoconstriction ( 5.5 + /- 4.9 % , 1.0 + /- 3.1 % , -5.1 + /- 1.8 % and 3.0 + /- 6.6 % ) . - pubmed
There was no effect of superoxide dismutase , catalase , desferrioxamine , NG - nitro - L - arginine methyl ester or saline in control lungs on albumin flux ( 0.10 + /- 0.04 , 0.11 + /- 0.01 , 0.1 + /- 0.01 , 0.12 + /- 0.01 and 0.11 + /- 0.01 respectively ) or palmitic : linoleic acid ratio ( -0.08 + /- 0.08 , 0.73 + /- 0.76 , -0.03 + /- 0.12 , 0.01 + /- 0.17 and 0.00 + /- 0.0 respectively ) . - pubmed
Flavocytochrome b558 of the NADPH oxidase which generates superoxide in phagocytic cells , is a alpha1 beta1 heterodimer of gp91phox and p22phox , which together form a membrane - spanning electron - transport chain that transfers electrons from NADPH in the cytosol to oxygen . - pubmed
The results indicated that while a temporary immunosuppression with CsA achieved an indefinite islet allograft survival , FA administered to recipients daily was not able to prevent islet allograft rejection across a major histocompatibility barrier . - pubmed
It is shown that mechanisms of reperfusion fibrillations ( RF ) are more sensitive than ones of RTS to changes of the intracellular calcium concentration . - pubmed
For example , RF have been easily suppressed by calcium concentration decreasing at any stage of the experiment : upon reperfusion , or during previous ischemia , whereas RTS have not been suppressed even under total removal of calcium from solutions either before initiation of ischemia or during following reperfusion . - pubmed
The analysis of RNAs from the virus - infected and PAP - treated protoplasts demonstrated that 25S rRNA was depurinated by PAP in the infected cells . - pubmed
These responses were blocked by inhibition of tyrosine kinase activity with genistein , suggesting that the increases in Ca2 + and tyrosine phosphorylation are functionally coupled . - pubmed
The transient was inhibited 25 - 40 % by L - type Ca2 + channel antagonists and inhibited 90 - 95 % by genistein . - pubmed
The sustained response was unaffected by L - channel antagonists and only slightly inhibited by genistein . - pubmed
The influx pathway during the transient probably involves an L - type Ca2 + channel that is regulated by tyrosine kinase activity . - pubmed
The pathways that participate in the sustained response are different because they are insensitive to l - channel antagonists and only slightly inhibited by genistein . - pubmed
Therefore , thapsigargin - mediated suppression of serotonin - activated release probably reflects depletion of Ca2 + from the sarcoplasmic reticulum , whereas genistein - mediated suppression probably reflects inhibition of tyrosine kinase linked release . - pubmed
In the inner membrane of liver mitochondria , three major 45Ca2 + -binding polypeptides : a protein of approximately 130 kDa ( carbamoyl - phosphate synthetase ) , a glycoprotein of 43 - 44 kDa ( previously considered as the calcium uniporter ) , and 29 - 30 kDa protein were found . - pubmed
One of them might be the natural Ca2 + -binding inhibitor of H +- ATPase . - pubmed
DNA adduct levels in lymphocytes were determined by the highly sensitive nuclease P1 - enhanced 32P - postlabeling assay . - pubmed
Hydrolytic cleavage of CAV to canaline ( CAN ) and urea is mediated by arginase . - pubmed
Modulation of GT-1 DNA - binding activity by calcium - dependent phosphorylation . - pubmed
Taken together , our results suggest that GT-1 may act as a molecular switch modulated by calcium - dependent phosphorylation and dephosphorylation in response to light signals . - pubmed
Serum Zn ( 2 +)-stimulated sphingomyelinase deficiency in type B Niemann - Pick disease . - pubmed
Influence of a lysine 331 counterion on the pK(a ) of aspartic acid 125 : evidence for a salt - bridge interaction and role in alpha ( 1b )- adrenergic receptor activation . - pubmed
The amino acids , K331 and D125 , which comprise this salt - bridge , should be closely associated with each other in the unbound form of the alpha ( 1b )- AR . - pubmed
If the charged state of D125 is important for agonist binding , then changing the type of amino acid at position 331 should decrease the acid strength of D125 , leading to epinephrine affinity changes for the alpha ( 1b )- AR . - pubmed
For all mutations of the alpha ( 1b )- AR where the positive charge at position 331 was eliminated , there was a significant increase in the pK(a ) ( congruent with 0.73 ) of an acidic amino acid(s ) . - pubmed
In this model , the binding capacity of lignin was equal to hemicellulose for PER , AZM and CLP , but it was significantly higher for CKL . - pubmed
Hemicellulose bound more CKL , AZM and CLP than did cellulose . - pubmed
In addition , the effect on aromatase expression of charcoal - stripped fetal calf serum , of quercetin and genistein and of tamoxifen and raloxifene was investigated in both cell lines . - pubmed
Cell death is significantly blocked by the caspase inhibitor zVAD - fmk and when coexpressed with E1B19 K , Bcl-2 or the retinoblastoma protein ( RB ) . - pubmed
Activation of MAP kinases requires phosphorylation of both Thr and Tyr in the catalytic domain by a family of dual - specificity kinases , called MEKs ( MAP kinase / ERK kinase ) . - pubmed
MEK1 is activated by phosphorylation at Ser218 and Ser222 by Raf . - pubmed
Mutation of these two sites to acidic residues , specifically [ Asp218 ] , [ Asp218 , Asp222 ] , and [ Glu218 , Glu222 ] , results in constitutively active MEK1 . - pubmed
PTX3 is a prototypic long pentraxin consisting of a C terminal 203 - amino acid pentraxin - like domain coupled with an N - terminal 178 - amino acid unrelated portion . - pubmed
In part these effects may involve a nucleotide - induced release of adenosine and the simultaneous activation of P1 and P2 receptors . - pubmed
A membrane - bound glucose dehydrogenase quinoenzyme [ which contains pyrroloquinoline quinone ( PQQ ) as the prosthetic group ] is involved in that oxidation . - pubmed
diazotrophicus PAL3 was grown in a chemostat with atmospheric nitrogen as the sole N source provided that the dissolved oxygen was maintained at 1.0 - 2.0 % air saturation . - pubmed
Here , the effects of db - cAMP in lung preservation were ultrastructurally investigated , and the ultrastructural changes before reperfusion were correlated with pulmonary function after reperfusion . - pubmed
In the fresh group ( n = 6 ) , the lungs were flushed with extracellular - type trehalose - containing ( ET - K ) solution and were reperfused immediately . - pubmed
In the control group ( n = 6 ) and db - cAMP group ( n = 5 ) , the lungs were flushed with ET - K solution and ET - K solution plus db - cAMP ( 2 mM ) , respectively , and were reperfused after cold preservation at 4 degrees C for 15 h. Before reperfusion , tissue was sampled and ultrastructurally analyzed by transmission electron microscopy . - pubmed
Dosing preparations included uncooked fresh sprouts ( with active myrosinase ) as well as homogenates of boiled sprouts that were devoid of myrosinase activity and contained either glucosinolates only or isothiocyanates only . - pubmed
Essential role of NHE3 in facilitating formate - dependent NaCl absorption in the proximal tubule . - pubmed
The virtually complete dependence of formate - induced NaCl absorption on NHE3 activity raises the possibility that NHE3 and the formate transporters are functionally coupled in the brush border membrane . - pubmed
dose - dependently suppressed neutrophil infiltration and nitric oxide ( but not KC and IL-1beta ) production in peritonitis . - pubmed
In a model of systemic endotoxemia , PJ34 pretreatment significantly reduced plasma levels of TNF - alpha , IL-1beta and nitrite / nitrate ( breakdown products of nitric oxide ) production . - pubmed
Delaying the start of PJ34 administration in the colitis model conferred significant protective effects , while in the arthritis model the post - treatment paradigm lacked protective effects . - pubmed
A series of novel synthetic derivatives , comprising monohydroxylated benzoperimidines and 2 - aminobenzoperimidines , allowed the establishment of an in vitro structure - activity relationship for a panel of leukemia sensitive , as well as P - gp dependent multidrug resistance ( MDR ) and multidrug resistance associated protein dependent resistance ( MRP ) resistant cell lines . - pubmed
The aim of this study was to investigate the role of inducible nitric oxide ( NO ) synthase ( iNOS ) and NO on the modulation of the inflammatory response caused by splanchnic ischemia and reperfusion . - pubmed
These results suggest that the induction of iNOS and NO production are essential for the upregulation of the inflammatory response in splanchnic ischemia / reperfusion and participate in end organ damage under these conditions . - pubmed
Combinations using an alpha2 - adrenoceptor agonist and ketamine have been shown to be effective for immobilization but are not reversible and can produce severe hypertension and prolonged or rough recoveries . - pubmed
We assessed the effect of endogenous GABA on ictal activity and neuronal firing rate in hippocampal slices from postnatal day 1 ( P1 ) to P30 . - pubmed
In extracellular recordings , there was a strong correlation between the postnatal age at which GABA ( A )- R antagonists decreased action potential frequency ( P23 ) and the age at which ictal activity could be induced by elevated potassium ( P23 ) . - pubmed
In addition , there was a strong correlation between the fraction of slices in which ictal activity was induced by elevated potassium concentrations and the fractional decrease in action potential firing when GABA(A)-Rs were blocked in the presence of ionotropic glutamate receptor antagonists . - pubmed
Finally , ictal activity induced by elevated potassium was blocked by the GABA ( A )- R antagonists bicuculline and SR-95531 ( gabazine ) and increased in frequency and duration by GABA ( A )- R agonists isoguvacine and muscimol . - pubmed
In bipolar cells , the number of Ca entry sites and ribeye - positive fluorescent spots is also strikingly similar to that of exocytic active zones but significantly less than the number of total exocytic sites including solitary fusion events outside active zones . - pubmed
[ Mg ( 2 +)]( i ) was increased after the stimulation with 100 microU / mL of insulin . - pubmed
After 60 seconds of insulin stimulation , the value of [ Mg ( 2 +)]( i ) was lower in the type 1 DM group compared with the control group ( 729 + /- 85 micromol / L v 1,078 + /- 67 micromol / L , P & lt;.005 ) . - pubmed
The activity of cytochrome oxidase ( COX ) , coupled to ATP production , reflects long - term plasticity in metabolic capacity . - pubmed
TUDCA inhibition of cholangiocarcinoma growth was blocked by BAPTA - AM , an intracellular Ca ( 2 + ) concentration ( [ Ca ( 2 +)]( i ) ) chelator , and H7 , a PKC - alpha inhibitor . - pubmed
TUDCA inhibited the activity of MAPK , and this inhibitory effect of TUDCA was abrogated by BAPTA - AM and H7 . - pubmed
TUDCA did not alter the activity of Raf-1 and B - Raf and the phosphorylation of MAPK p38 and JNK / stress - activated protein kinase . - pubmed
TUDCA inhibits Mz - ChA-1 growth through a signal - transduction pathway involving MAPK p42 / 44 and PKC - alpha but independent from Raf proteins and MAPK p38 and JNK / stress - activated protein kinases . - pubmed
A number of drugs are used in neurodegenerative disorders including antidepressants , benzodiazepines , antipsychotics , acetylcholinesterase inhibitors and levodopa . - pubmed
Co - administration of monoamine oxidase inhibitors and opioids should be avoided as it can cause agitation , muscular rigidity , sweating and hyperpyrexia . - pubmed
Four broad groups of investigational therapeutic agents appear promising for future therapy : modified interferons and ribavirins , immunomodulators , viral life - cycle targets , and antifibrotic agents . - pubmed
Heme oxygenase , steering dioxygen activation toward heme hydroxylation . - pubmed
The activation of dioxygen by heme oxygenase proceeds via formation of an obligatory ferric hydroperoxide intermediate ( FeIII - OOH ) , as is the case in the activation of dioxygen by monooxygenase enzymes . - pubmed
This review summarizes current understanding of the structural and dynamic properties in heme oxygenase that channel the reactivity of the FeIII - OOH intermediate toward heme hydroxylation rather than oxoferryl formation . - pubmed
Differences in mechanism between heme hydroxylation , as carried out by heme oxygenase , and the coupled oxidation process , are also addressed . - pubmed
Photochromic agents as tools for protein structure study : lapachenole is a photoaffinity ligand of cytochrome P450 3A4 . - pubmed
These attributes of the chromenes inspired testing of their potential to label cytochrome P450 3A4 in a light - dependent fashion . - pubmed
Access to the protein active site by lapachenole was demonstrated with the molecule 's ability to competitively inhibit P450 3A4 - mediated oxidative metabolism of midazolam with an IC(50 ) value of 71 microM. - pubmed
These results clearly demonstrate that chromenes are effective photoaffinity reagents for the cytochrome P450 superfamily of enzymes and probably other proteins as well . - pubmed
Kahalalide F induces necrosis - like cell death that involves depletion of ErbB3 and inhibition of Akt signaling . - pubmed
Kahalalide F ( KF ) is a novel marine - derived antitumor agent that is currently undergoing phase II clinical trials . - pubmed
Several markers of caspase - dependent apoptosis , such as phosphatidyl - serine externalization , cytochrome c release , and caspase-3 and poly -( ADP - ribose ) polymerase cleavage were negative after KF exposure . - pubmed
EPA505 and related strains in PAH - contaminated soils , a new 16S rRNA gene - based primer set was designed . - pubmed
Applying the new primer set , 16S rRNA gene fragments which were 99 - 100 % similar to the corresponding gene of strain EPA505 were amplified from four of five PAH - contaminated soils . - pubmed
The proteins bound on the interacting RNA are eluted and subjected to SDS - PAGE , and the Coomassie - stained protein bands are cut and subjected to mass spectrometry ( MS ) analysis . - pubmed
Exogenous NE ( 3 micromol / l ) , alpha , beta - methylene ATP ( 1 micromol / l ) , neuropeptide Y ( NPY , 0.1 micromol / l ) , CGRP ( 0.1 micromol / l ) , vasoactive intestinal peptide ( VIP , 0.1 micromol / l ) , and substance P ( SP , 0.1 micromol / l ) had no effect on the basal release of beta - NAD , suggesting that the overflow of beta - NAD is evoked by neither the sympathetic neurotransmitters NE , ATP , and NPY , nor the neuropeptides CGRP , VIP , and SP . - pubmed
Botulinum neurotoxin A ( BoNTA , 0.1 micromol / l ) abolished the evoked release of NE , ATP , and beta - NAD at 4 Hz , suggesting that at low levels of neural activity , release of these neurotransmitters results from N - ethylmaleimide - sensitive factor attachment protein receptor / synaptosomal - associated protein of 25 kDa - mediated exocytosis . - pubmed
Immune reconstitution was noted to occur at a significantly faster rate in the secondary recipients , and without the occurrence of graft - versus - host disease ( GVHD ) , despite the presence of donor cells mismatched for 1 to 3 HLA antigens . - pubmed
Nanosensor - expressing plants can be used to assess glucose flux differences between cells , invertase - mediated sucrose hydrolysis in vivo , delivery of assimilates to roots , and glucose flux in mutants affected in sugar transport , metabolism , and signaling . - pubmed
[ Labeling of CDTPA - dianhydride - coupled CD45 monoclonal antibody with yttrium-90 ] . - pubmed
OBJECTIVE : To explore the methods for labeling CDTPA - coupled CD45 monoclonal antibody ( mAb ) with yttrium-90 ( ( 90 ) Y ) for potential acute myeloid therapy . - pubmed
METHODS : CD45 mAb was labeled with ( 90 ) Y by CDTPA and the labeling rate , radiochemical purity , final specific activity , and immunological activity of the mAb were detected . - pubmed
CONCLUSION : ( 90 ) Y - CDTPA - CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia . - pubmed
This is due to the distinct content of the X and Y - chromosomes in both genes and regulatory sequences , SRY being the key regulator Hormones ( AMH , testosterone , Insl3 ) secreted by the foetal testis ( gonadal sexual dimorphism ) , impede Muller duct development , masculinize Wolff duct derivatives and are involved in testicular descent ( hormonal sexual dimorphism ) . - pubmed
In addition , the correlation of both carbohydrate antigens expression was evaluated with microvessel density ( MVD ) . - pubmed
RESULTS : Expressions of sialyl Lewis antigens and MVD were associated with several clinicopathological features that reflect the tumor aggressiveness in esophageal cancer . - pubmed
Essential role of protein kinase C zeta in transducing a motility signal induced by superoxide and a chemotactic peptide , fMLP . - pubmed
Amino acid homology to other LFYs is 43 - 55 % ; conserved and variable regions were similar to others . - pubmed
Known features of transcription factors exhibited by ScLFY were an acidic domain in the central variable region , a proline - rich variable - region , and glutamate - rich regions . - pubmed
Independent predictors of drug resistance were the earlier calendar year , prior use of suboptimal nucleoside analogue therapy , male sex and higher CD4 levels at testing . - pubmed
Cd and Pb(EDTA ) produce the strongest increases of OASTL activity , with the higher enhancement seen in roots , in parallel with the higher metal accumulation . - pubmed
Arsenic produced an increase of root enzyme activity , whereas Cu produced a decrease , mainly in shoots . - pubmed
OASTL transcripts were increased by As , Cd , and Pb , especially in roots , where metal accumulation was maximal , while Cu produced a decrease in the transcript levels . - pubmed
More than 20 % of all subjects were using their inhaler device incorrectly ( Turbuhaler 37.1 % ; Metered - dose inhaler 28.1 % ; Diskus 21.3 % ; Aerolizer 7.4 % ) and this was associated with a 0.84 - point increase in ACS . - pubmed
Exercise - induced protection against myocardial apoptosis and necrosis : MnSOD , calcium - handling proteins , and calpain . - pubmed
We hypothesized that an exercise - induced increase in MnSOD would provide cardioprotection by attenuating IR - induced oxidative modification to critical Ca ( 2 +)-handling proteins , thereby decreasing calpain - mediated cleavage of these and other proteins attenuating cardiomyocyte death . - pubmed
In addition , exercise prevented IR - induced calpain activation as well as the oxidative modification and calpain - mediated degradation of myocardial Ca ( 2 +)-handling proteins ( L - type Ca ( 2 + ) channels , phospholamban , and sarcoplasmic / endoplasmic reticulum calcium ATPase ) . - pubmed
These results suggest that MnSOD provides cardioprotection by attenuating IR - induced oxidation and calpain - mediated degradation of myocardial Ca ( 2 +)-handling proteins , thereby preventing myocardial apoptosis and necrosis . - pubmed
There are seven related NADPH oxidases ( NOX 1 - 5 , DuoNOX 1,2 ) which can be activated in various ways to produce superoxide and hydrogen peroxide at the plasma membrane . - pubmed
For this purpose , a post hoc subanalysis of the randomized , placebo - controlled CAPRAF ( Candesartan in the Prevention of Relapsing Atrial Fibrillation ) trial was performed . - pubmed
OBJECTIVE : The purpose of our study was to use the total choline - containing compound ( tCho ) peak integral as a marker of malignancy in breast MR spectroscopy ( MRS ) . - pubmed
Identification of amino acid residues of the pheromone - binding domain of the transcription factor TraR that are required for positive control . - pubmed
Here , we constructed 117 mutants , altering 103 surface - exposed amino acid residues of the TraR N - terminal domain . - pubmed
Amino acid residues required for activation of both promoters probably contact the C - terminal domain of the RNA polymerase alpha subunit , while residues required only for traI promoter activation may contact another RNA polymerase component . - pubmed
For instance , the high - zinc brass alloys ( with the highest levels of tin and zinc and the lowest melting temperature ) correspond to most of the Picasso sculptures , correlate with the Valsuani foundry , and are associated with the most recent sculptures ( post - WWII ) and with the lost - wax casting method . - pubmed
The accumulative penetration of DXM and AB within skin flap were also detected at 2 and 6 hours after application of 2 g of compound gel containing 3%AZ/2%PG ( group A2 ) and peritoneal injection AB ( 2 mg / kg ) / DXM ( 5 mg / kg ) ( group B2 ) . - pubmed
After 2 and 6 hours , the quantities of DXM and AB in skin flap of group A2 were significantly higher than that of group B2 ( P & lt ; 0.05 ) . - pubmed
Activity of the multikinase inhibitor sorafenib in combination with cytarabine in acute myeloid leukemia . - pubmed
We investigated a treatment strategy combining sorafenib , a multikinase inhibitor with limited single - agent activity in AML , and cytarabine , a key component of AML chemotherapy . - pubmed
RESULTS : Sorafenib at 10 muM inhibited cell viability , proliferation and ERK1 / 2 signaling , and induced apoptosis in all cell lines studied . - pubmed
In this work a protocol for the enzyme - assisted removal of non - triple helical polypeptide entities in atelocollagen solutions was conceived , using pronase as scavenger , in the presence of poly ( ethyleneglycol ) ( PEG 10000 ) as a crowding agent , trimethylamine - N - oxide dihydrate , ( TMAO ) as kosmotropic agent and anhydrous sodium sulfate as anti - chaotrope salt . - pubmed
Total cAMP response element binding protein ( CREB ) concentrations ranged from 0.8 ng / ml to 1 ng / ml , whereas the proportions of phospho - CREB versus total CREB differed between the cell lines . - pubmed
Basic fibroblast growth factor ( FGF-2 ) and epidermal growth factor ( EGF ) stimulated cAMP generation , whereas leukaemia inhibiting factor reduced some of their effects . - pubmed
Forskolin ( 0.05 and 1 mM ) acted in synergy with FGF-2 and EGF ; however , it caused pronounced donor to donor differences in the increase of cAMP and phospho - CREB levels . - pubmed
Heterogeneity of donor cell responses to stimulation of the cAMP pathway indicates the need for wide safety margins for S - MSC use in drug screening ; nevertheless , knowledge of this heterogeneity might be useful for the design of donor - specific cell therapy . - pubmed
Salinomycin can effectively kill ALDH(high ) stem - like cells on gastric cancer . - pubmed
Importantly , ALDH(high ) cancer cells are relatively sensitive to salinomycin when compared to ALDH(low ) cancer cells ( P<0.01 ) . - pubmed
Hemoglobin ( Hb)-based oxygen carriers ( HBOCs ) are being developed as resuscitative fluids for use in multiple medical applications and in lieu of blood transfusion . - pubmed
We evaluated thyroid hormone levels , ultrasound examination of thyroid and urine iodine concentration ( UIE ) at each trimester and at 3 - month post - partum period ( PP ) . - pubmed
During weight recovery , OC and CTX were independently and positively associated with weight , weight gain , IGF-1 and oestradiol . - pubmed
Lyophilisomes were prepared by a freezing , annealing , and lyophilization method and a cystein - elongated TAT peptide was conjugated to the lyophilisomes using a heterobifunctional linker . - pubmed
Here we place the FLT3 - ITD upstream of BTK in AML and show that the BTK inhibitor ibrutinib inhibits the survival and proliferation of FLT3 - ITD primary AML blasts and AML cell lines . - pubmed
Furthermore ibrutinib inhibits the activation of downstream kinases including MAPK , AKT and STAT5 . - pubmed
Finally we report that ibrutinib reverses the cyto - protective role of BMSC on FLT3 - ITD AML survival . - pubmed
Progesterone concentration was not different ( P & gt ; 0.05 ) in S1 compared to S2 . - pubmed
This report analyses the potential of [ ( 68 ) Ga ] Glu - urea - Lys ( Ahx )- HBED - CC ( ( 68 ) Ga - PSMA-11 ) , a new positron emission tomography ( PET ) tracer targeting prostate - specific membrane antigen ( PSMA ) for prostate cancer staging and individualized radiotherapy planning . - pubmed
Staging results of conventional imaging , including bone scintigraphy , CT or MRI , were compared with ( 68 ) Ga - PSMA ligand PET / CT results and the influence on radiotherapeutic management was quantified . - pubmed
RESULTS : ( 68 ) Ga - PSMA ligand PET / CT had a dramatic impact on radiotherapy application in the presented cohort . - pubmed
Diabetic status was monitored using blood and urine , and at the end , harvested kidneys were used to study the amelioration of glycoprotiens ( collagen ) and HS by enzymatic digestion , spectrophotometric , hydroxyproline and agarose electrophoretic methods . - pubmed
Increased collagen content in diabetic group was observed by hydroxyproline estimation ( 24 % ) and periodic acid - Schiff 's ( PAS ) staining . - pubmed
Results of monosaccharide composition indicated that CSAP and its neutral fractions mainly consisted of mannose and galactose ( 79.45 - 97.52 % ) with M / G ratios of 0.94 - 1.48 , typical for galactomannans , which were demonstrated by FT - IR . - pubmed
Both Shank3(e4 - 9 ) HET and KO demonstrated a significant reduction in NMDA / AMPA ratio at excitatory synapses onto striatal medium spiny neurons . - pubmed
In HSF-1(-/-)/LDLr(-/- ) liver , higher cholesterol 7alpha - hydroxylase ( CYP7A1 ) and multidrug transporter [ MDR1 / P - glycoprotein ( P - gp ) ] gene expressions were observed , consistent with higher bile acid transport and larger hepatic bile ducts . - pubmed
The molecular weight and the Michaelis constants were determined ; the effect of hydrogen ions on the forward and reverse MDH reaction was studied . - pubmed
The results of the study demonstrated that the enzyme consists of subunits ; the molecular weight of subunits was determined by SDS - PAGE . - pubmed
The model takes the synergistically acting light / dark cycles and feeding rhythms as inputs and incorporates the activity of sirtuin 1 , a cellular energy sensor and a metabolic enzyme activated by nicotinamide adenine dinucleotide . - pubmed
Other abnormalities include high renin , secondary hyperaldosteronism , and elevated levels of prostaglandin E2 . - pubmed
FMD was higher in the isoflavone group than in the control group after 24 - week therapy ( treatment effect 2.0 % , 95 % CI 0.18 - 3.0 , P = 0.01 ) . - pubmed
The baseline differences were adjusted in FMD and the isoflavone therapies were closely associated with the reduction of FMD impairment at 24 weeks ( odds ratio 0.30 , 95 % CI 0.14 - 0.85 , P = 0.01 ) . - pubmed
The effects of isoflavone on brachial FMD were negatively associated with base FMD ( r = -0.65 , P & lt ; 0.01 ) . - pubmed
Furthermore , isoflavone therapy caused a significant increase in the levels of erythroid - derived 2 - like 2 ( Nrf2 ) , superoxide dismutase ( SOD ) and a significant decrease in serum levels of C - reactive protein , 8 - isoprostane , malondialdehyde , interleukin-6 and tumor necrosis factor alpha . - pubmed
In contrast , the isoflavones had no effects on the levels of oxidation - related molecules when Nrf2 was silenced . - pubmed
PURPOSE : In the phase III EMILIA study , trastuzumab emtansine ( T - DM1 ) significantly improved progression - free survival ( PFS ) and overall survival ( OS ) versus capecitabine plus lapatinib ( control ) in previously treated human epidermal growth factor receptor 2 - positive advanced breast cancer . - pubmed
Plasma was analysed for triglycerides , glucose , insulin , and non - esterified fatty acids ( NEFA ) . - pubmed
34.7 % of patients never took vitamin D within 12 months after surgery , 26.2 % took vitamin D inconsistently , and 39.1 % took vitamin D consistently . - pubmed
In this study , we have elucidated and verified the chemical structure of two major equine arylpropionamide - based SARM metabolites using a combination of chemical synthesis and liquid chromatography - mass spectrometry ( LC - MS ) analysis . - pubmed
Cannabinoid - induced cell death in endometrial cancer cells : involvement of TRPV1 receptors in apoptosis . - pubmed
Finally , these effects were dependent on TRPV1 activation and intracellular calcium levels . - pubmed
Selective targeting of TPRV1 by AEA , CBD , or other stable analogues may be an attractive research area for the treatment of estrogen - dependent endometrial carcinoma . - pubmed
The WT MRP4 molecular structure confirmed and ameliorated the general knowledge about the transmembrane helices and the two nucleotide binding domains . - pubmed
They were then randomly divided into 6 groups : control group ( I ) , chlorhexidine ( II ) , Khadiradi vati ( III ) , Dashansanskar churana ( IV ) , Neem ( V ) , and Apamarga ( VI ) ; based on the drugs used by patients for the maintenance of oral hygiene after oral prophylaxis . - pubmed
PYT and some of its derivatives , including phytanic acid ( PA ) , exert a wide range of biological effects . - pubmed
PPARs- and NF - kappaB - mediated activities are also discussed as mechanisms responsible for some of the bioactivities of PYT . - pubmed
Carbamazepine ( CBZ ) , oxcarbazepine ( OXC ) , and eslicarbazepine acetate ( ESL ) belong to the dibenzazepine family of antiepileptic drugs and are all thought to primarily act as sodium channel blockers ( SCBs ) . - pubmed
Negative Impacts of Biochars on Urease Activity : High pH , Heavy Metals , Polycyclic Aromatic Hydrocarbons , or Free Radicals ? - pubmed
The results showed that biochars inhibited urease activity ( indicated by the NH4 + generation ) . - pubmed
Released heavy metals and polyaromatic hydrocarbons explained the 20 % inhibition of urease activity in biochar supernatants ( 200 C ) but could not explain the 70 % inhibition in particle systems ( 400 C ) . - pubmed
The levels of protein carbonyl and nitrotyrosine , common oxidative damagers of urease , were high in particle systems but very low in biochar supernatants . - pubmed
The observed inhibition of urease activity may be the result of oxidative reactions with free radicals on the biochar surface or oxidative reactions with reactive oxygen species promoted by free radicals . - pubmed
This minireview summarizes what is currently known about the sources and fate of TMA in marine environments and the different pathways and enzymes involved in TMA metabolism in marine bacteria . - pubmed
The formation of 2a is initiated by the one - electron reduction of [ 1a]+ , followed by a reversible intramolecular rearrangement terminating with the irreversible release of CpH ( NMR and gas chromatography - mass spectrometry ) and Fe [ electron paramagnetic resonance ( EPR ) and magnetometry ] . - pubmed
The key intermediate iron ( I ) ferraferrocene ( 3 ) was detected by EPR and IR spectroelectrochemistry , while the related species 3 - H-3 was isolated after the addition of a hydrogen source and then identified by X - ray diffraction . - pubmed
The functionalized diiron compounds 4 ( 52 % yield ) and 5 ( 62 % ) were afforded through the activation of O2 and S8 by a radical intermediate along the reductive pathway of [ 1a]+ . - pubmed
Rapid Layer Technology ( RLT ) uses computer - aided design / computer - aided manufacturing ( CAD / CAM ) to manufacture a veneer layer that is adhesively bonded to the zirconia framework , avoiding firing steps during the fabrication process and thus preventing build - up of residual stresses . - pubmed
For the latter , the veneers were CAD / CAM fabricated using a feldspathic reinforced - glass ( Vitablocs Mark II ; n = 16 ) or a polymer - infiltrated reinforced - glass network ( Enamic ; n = 16 ) and adhesively bonded to the zirconia frameworks . - pubmed
Recent findings have identified salmon calcitonin ( sCT ) , an amylin receptor agonist and analogue of endogenous amylin , as a potential regulator of alcohol - induced activation of the mesolimbic dopamine system and alcohol consumption . - pubmed
Providing that the role of amylin signalling in alcohol - related behaviours remains unknown , the present experiments investigate the effect of sCT on these behaviours and the mechanisms involved . - pubmed
Collectively , our data suggest that amylin signalling , especially in the NAc , may contribute to reduction of various alcohol - related behaviours . - pubmed
Correlation analysis corrected for the presence of confounding variables ( riluzole , age at onset , site of onset , gender ) was tested between PRL ( Progression Rate at the Last visit ) and LMVs , and a statistically significant value was found ( Pearson partial correlation r = 0.407 , p = 0.006 ) . - pubmed
Activated PPARs participate in the regulation of lipid and glucose metabolism and also affect cellular proliferation , differentiation , and apoptosis , and the immune responses . - pubmed
Also , the serum levels of fasting blood sugar ( P & lt ; 0.001 ) , HbA1c ( P = 0.04 ) , cholesterol ( P = 0.03 ) , triglycerides ( P = 0.01 ) , blood urea nitrogen ( P = 0.03 ) , and pro - inflammatory cytokines including IL-1beta , IL-6 , and TNF - alpha were significantly higher in BN candidates for LSG ( P & lt;= 0.001 ) . - pubmed
Efficacy and safety of TNF blockers and of ustekinumab in palmoplantar pustulosis and in acrodermatitis continua of Hallopeau . - pubmed
OBJECTIVES : To evaluate the real - life efficacy of tumor necrosis factor blockers and ustekinumab in PPP and in ACH . - pubmed
CONCLUSIONS : We found no difference in efficacy between TNF blockers and ustekinumab and among the three different TNF blockers in real life for PPP or ACH , which reveals the heterogeneity of clinical response to biologics in pustular psoriasis as compared with plaque - psoriasis . - pubmed
In vitro effect of some nucleoside reverse transcriptase inhibitors against HSV-1 replication . - pubmed
OBJECTIVE : The current study was designed to investigate the effects of some nucleoside reverse transcriptase inhibitors ( NRTIs ) on HSV-1 infection . - pubmed
MATERIALS AND METHODS : Initially , the SwissTargetPrediction server was used to predict the interactions between HSV-1 thymidine kinase and acyclovir , stavudine , zidovudine , didanosine , and entecavir . - pubmed
Transmission electron microscopy could detect no disruption of normal endocytosis at the apical surface of Tmc1Delta;Tmc2Delta hair cells in vitro . - pubmed
Besides complement inhibition , C1 - INH also prevents FXII activation , a very early event of contact phase activation at the crossroads of coagulation and inflammation . - pubmed
The analysis of various blood and plasma parameters revealed that C1 - INH - containing coatings were able to markedly reduce FXIIa activity compared to heparin coating . - pubmed
Combined C1 - INH / heparin coatings yielded similarly low levels of thrombin - antithrombin III complex formation as heparin coating . - pubmed
Significant differences ( p < 0.05 ) were only found in the expression of glutathione transferase , which was increased almost 3 times in the group treated with beta - glucans as compared with the control group , and 1.5 times as compared with the group treated with acrylamide . - pubmed
, catalase ( CAT ) , peroxidase ( POD ) , and super oxide dismutase ( SOD ) ) , malondialdehyde ( MDA ) , and Pb concentration in plants subjected to different Pb concentrations . - pubmed
Si NPs minimized accumulation of MDA in plant tissues and adjusted the activities of POD , CAT , and SOD in plants under Pb stress . - pubmed
Overall , Si NP foliar application might be a suitable approach in reducing the Pb concentrations in plants . - pubmed
Twelve healthy males ( stature : 1.75 + - 0.08 m ; body mass : 67.5 + - 6.3 kg ; age : 21.0 + - 1.4 years ; maximal oxygen consumption : 45.1 + - 7.0 ml.kg.min-1 ) attended four separate laboratory visits . - pubmed
Cycling TTE increased for SBC2 ( +20.2 s ; p = 0.045 ) and SBC3 ( +31.9 s ; p = 0.004 ) compared to PLA . - pubmed
GI upset was exacerbated post - exercise after SBC3 for nausea compared to SBC2 and PLA ( p < 0.05 ) , whilst SBC2 was not significantly different to PLA for any symptom ( p > 0.05 ) . - pubmed
Biochemical methods were used ( RNA , DNA , total protein content , liver antioxidant activity , insulin and glucose levels in the blood ) , transmission electron microscopy , morphometric analysis of mitochondria ( number , average and total area ) . - pubmed
Regulation of mitochondrial and microsomal phospholipid synthesis by liver fatty acid - binding protein . - pubmed
Recently we have detected and partially purified a 15 - kDa cytosolic L - alpha - lysophosphatidic acid ( LPA )- binding protein ( LPABP ) , which stimulates export of LPA from mitochondria ( Vancura , A. , Carroll , M. A. , and Haldar , D. ( 1991 ) Biochem . - pubmed
By Western immunoblot analysis , amino acid sequence analysis , and binding characteristics we have shown that LPABP is identical with liver fatty acid - binding protein ( L - FABP ) . - pubmed
This protein binds LPA , and stimulates mitochondrial and microsomal glycerophosphate acyltransferase ( GAT ) and the export of LPA from both the organelles . - pubmed
Taken together , these findings are suggestive that L - FABP plays a major role in mitochondrial and microsomal phospholipid metabolism by regulating both the synthesis and utilization of LPA . - pubmed
Three analogues with restricted flexibility were designed to study the active conformation of verapamil during interaction with the slow calcium channel . - pubmed
Interferon alpha and etretinate combination treatment of cutaneous T - cell lymphoma . - pubmed
A birth control vaccine incorporating a synthetic peptide antigen representing the aminoacid sequence 109 - 145 of the C - terminal region of the beta subunit of human chorionic gonadotropin ( hCG - beta ) was submitted to a phase 1 clinical trial . - pubmed
We conclude that the acutely preovulatory expression of CA-125 in serum and FF as determined by EIA is low and not significantly enhanced by OH . - pubmed
Serum aminoterminal propeptide of type III procollagen ( PIIINP ) was studied in 73 psoriatics receiving methotrexate and in 11 selected for trial with methotrexate or etretinate . - pubmed
PIIINP is not specific for the liver , but the study indicates that the number of liver biopsies can be reduced in psoriatics on methotrexate who have normal levels of PIIINP . - pubmed
Pharmacological activity of N - methyl - carbamylcholine , a novel acetylcholine receptor agonist with selectivity for nicotinic receptors . - pubmed
N - Methyl - carbamylcholine ( also called N - methyl - carbachol ) is an analogue of the mixed muscarinic - nicotinic acetylcholine receptor agonist , carbachol . - pubmed
To determine whether N - methyl - carbachol acts as an agonist or an antagonist at nicotine and/or muscarinic receptor sites , the present study examined the pharmacological activity of this compound on some cholinergically innervated tissues . - pubmed
The results suggest that the cytotoxicity , clastogenicity and mutagenic activity of the amsacrine analogues is mediated by similar mechanisms , probably involving the enzyme DNA topoisomerase II . - pubmed
Compared with the control group , lovastatin alone decreased the plasma total C value by 36 % ( p = NS ) , the LDL - C level by 35 % ( p = NS ) , hepatic C content by 29 % ( p = NS ) , and hepatic C synthesis by 52 % ( p = NS ) . - pubmed
Compared with the control group , the combination of PIB and lovastatin decreased the plasma total C level by 78 % ( p less than 0.005 ) , the LDL - C level by 74 % ( p less than 0.025 ) , and hepatic C content by 58 % ( p less than 0.05 ) ; however , the hepatic C synthesis increased by 490 % ( p less than 0.005 ) compared with the control group and by 110 % ( p less than 0.05 ) compared with PIB alone . - pubmed
We conclude that both PIB and lovastatin lower plasma total C and lipoprotein C fractions . - pubmed
The polar head group that was released by treating an insulin - sensitive glycophospholipid with a phosphatidylinositol - specific phospholipase C ( PI - PLC ) stimulated pyruvate dehydrogenase ( PDH ) in both subcellular and whole cell assays . - pubmed
The effect of the polar head group on PDH in the subcellular assay was blocked by sodium fluoride , suggesting that the polar head group activated the PDH phosphatase . - pubmed
The PDH - stimulating activity eluted after bacitracin but ahead of ATP during gel filtration chromatography , and it was destroyed by exposure to NH4OH or alkaline phosphatase and by boiling in water . - pubmed
Glucagon -( 1 - 21 )- peptide stimulated [ 3H ] thymidine incorporation of mucosal cells significantly in a dose - dependent manner . - pubmed
Then glucagon - related peptides which have common sequences with glucagon -( 1 - 21 )- peptide were also tested . - pubmed
The sulfonamides are separated from each other and from co - extracted materials on a C-18 reverse - phase column and detected at 450 nm following post - column derivatization with dimethylaminobenzaldehyde . - pubmed
Sulfamerazine is a good internal standard for sulfamethazine ; neither is ideal for sulfathiazole.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
L364718 ( 20 nmol / kg ) caused a potent inhibition of CCK-8 stimulated pancreatic protein , amylase and trypsin secretion but not of volume and bicarbonate secretion . - pubmed
Release of PP by CCK was also significantly suppressed by L364718 . - pubmed
The possible mechanism of phosphatidic acid - mediated cell stimulation and its relation to other growth factors are discussed . - pubmed
Based on SDS PAGE and the action of neuraminidase , the acetylcholine receptor - inducing activity ( ARIA ) appears to be a 42,000 - D glycoprotein . - pubmed
ARIA was extracted in a trifluoroacetic acid - containing cocktail and purified to homogeneity by reverse - phase , ion exchange , and size exclusion high pressure liquid chromatography . - pubmed
The 42 - kD ARIA is extremely stable : it was not destroyed by exposure to intact myotubes , low pH , organic solvents , or SDS . - pubmed
The effect of ARIA is apparently independent of the state of activity of the target myotubes as its effect on receptor incorporation added to that of maximal concentrations of tetrodotoxin . - pubmed
Esterases of the polymorphonuclear leukocyte capable of hydrolyzing acetyl DL - phenyl - alanine beta - naphthyl ester . - pubmed
Two of these esterases , esterase 1 and esterase 2 , are inhibited by various p - nitrophenyl ethyl phosphonate esters . - pubmed
The third esterase , esterase 3 , differs from the other two by its inability to be inactivated by any of the phosphonates no matter how high the concentration of phosphonate or prolonged the period of incubation of cell with phosphonate . - pubmed
The inhibition profiles of esterase 1 are distinctly different from those of esterase 2 when the two esterases are tested with the phenylalkylphosphonates , chloroalkylphosphonates , and alkylphosphonates . - pubmed
The inhibition profile of esterase 1 is essentially the same as that of the activatable esterase of chemotaxis obtained previously when the same three homologous series of phosphonates were tested for their ability to protect against deactivation by the chemotactic factor or give chemotactic - dependent inhibition . - pubmed
After sedimentation of virus - containing sera through a sucrose density gradient , colony inhibitory activity is present in the particulate fraction and separate from serum immunoglobulins . - pubmed
The components of the supernatant responsible for these activities have a molecular weight between 30,000 and 60,000 , and have the characteristics of non - specific factors : they are genetically unrestricted , and do not bind to either antigen or anti - DR affinity columns . - pubmed
While removing iron and labile sulphur from ferredoxin reaction center the reduction wave of Fe - S bonds with E 1/2 = -0.33 V ( N. H. E. ) transforms into the reduction wave of S - S bonds with E 1/2 = -0,39 V at pH = 7 . - pubmed
ZAP-70 binding specificity to T cell receptor tyrosine - based activation motifs : the tandem SH2 domains of ZAP-70 bind distinct tyrosine - based activation motifs with varying affinity . - pubmed
Engagement of the T cell antigen receptor ( TCR ) results in activation of several tyrosine kinases leading to tyrosine phosphorylation of protein substrates and activation of multiple biochemical pathways . - pubmed
TCR - mediated activation of the src - family kinases , Lck and Fyn , results in tyrosine phosphorylation of the TCR zeta and CD3 chains . - pubmed
The site of phosphorylation in these chains is the tyrosine - based activation motif ( TAM ) , a 15 - 16 amino acid module containing two tyrosine residues . - pubmed
Tyrosine - phosphorylated TAMs serve as targets for binding of the zeta - associated protein ( ZAP-70 ) tyrosine kinase via its tandem SH2 domains . - pubmed
Similar studies demonstrated that the ZAP-70 tandem SH2 domain can bind a TCR zeta 3 TAM peptide in which both tyrosine residues are phosphorylated : Little or no binding was observed with peptides phosphorylated at only one tyrosine residue , or a nonphosphorylated peptide . - pubmed
All four doubly tyrosine phosphorylated TAM peptides cross - compete with each other for binding to the tandem SH2 domains of ZAP-70 . - pubmed
The results provide further evidence that the ZAP-70 interaction with the TCR requires prior phosphorylation of both tyrosine residues within a TAM motif . - pubmed
Binding of ZAP-70 to phospho - TAMs is notable for the high level of cooperativity between the two SH2 domains , which individually demonstrate low affinity interaction with the ligand . - pubmed
Affinity differences of as much as 30 - fold indicates a significant specificity of interaction of ZAP-70 SH2 domains for different phospho - TAMs . - pubmed
In another example involving targeted cytosine deaminase for the generation of 5 - fluorouracil , it is shown that as much as 17 times more drug can be delivered within a tumor compared to when 5 - fluorouracil is administered alone . - pubmed
The in vivo effect of doxycycline treatment on matrix metalloproteinases in reactive arthritis . - pubmed
The functional implications of these genomic footprinting data and the tissue - specific CpG methylation patterns of the beta - like globin promoters we obtained by genomic sequencing are discussed in terms of positive and negative regulation of the human beta - like globin switch during erythroid development . - pubmed
RESULTS : There were inverse relationships between survival time on one hand and plasma values of noradrenaline ( r = -0.49 ; P & lt ; 0.01 ) and atrial natriuretic peptide ( r = -0.49 ; P & lt ; 0.01 ) on the other hand . - pubmed
There was a significant correlation between the plasma values of noradrenaline and atrial natriuretic peptide ( r = 0.50 ; P & lt ; 0.01 ) . - pubmed
No significant differences were observed in the peak serum concentrations of amylase between the groups treated with and treated without ONO-5046 . - pubmed
ONO-5046 significantly decreased the peak serum lipase concentration . - pubmed
However , pretreatment with ONO-5046 significantly inhibited the rise in serum CINC concentrations after reperfusion . - pubmed
Pretreatment with ONO-5046 also significantly inhibited the expression of CINC mRNA transcripts in the graft . - pubmed
However , ONO-5046 decreased CINC production by peritoneal macrophages in response to neutrophil elastase . - pubmed
Preclinical profile of the mixed 5 - HT1A / 5 - HT2A receptor antagonist S 21,357 . - pubmed
This study evaluated the pharmacological and behavioral effects of S 21,357 , a drug with high affinity for both 5 - HT1A and 5 - HT2A receptors . - pubmed
The drug behaved as antagonist at both 5 - HT1A autoreceptors and postsynaptic 5 - HT1A receptors , as it prevented the inhibitory effect of lesopitron on the electrical discharge of the dorsal raphe nucleus ( DRN ) 5 - HT neurons and the activity of forskolin - stimulated adenylate cyclase in hippocampal homogenates . - pubmed
When probed with CsC1 gradients the relative chloride permeability ( PC1 / PCs ) was estimated as 0.14 for GluR - B(R ) and 0.74 for GluR-6 ( R )- subunit channels . - pubmed
Trans - FA have consistently been shown to elevate LDL - cholesterol and apolipoprotein B to a similar extent as C12 - C16 saturated fatty acids . - pubmed
[ 3H ] WIN 35,428 is a cocaine analog that is useful for studying the distribution and density of the dopamine transporter in striatum and other brain regions . - pubmed
We have previously shown that fibroblasts from ultra - violet ( UV ) hypersensitive xeroderma pigmentosum patients ( XP ) are markedly deficient in catalase activity resulting in high intracellular levels of hydrogen peroxide ( H2O2 ) following UV irradiation . - pubmed
No direct correlation between catalase activity and repair ability was found since XP variant cells which are proficient in nucleotide excision repair ( NER ) showed activities as low as those found in NER deficient classical XP groups A and D. However , in contrast to the skin cancer prone XP patients , another NER deficient syndrome , trichothiodystrophy ( TTD ) , which does not exhibit any cancer predisposition , was found to present normal catalase activity . - pubmed
These results incited us to examine the structure / function relationship of the tetrameric active enzyme form of catalase ( which is the only one able to carry out H2O2 dismutation ) with its cofactor NADPH . - pubmed
In the present study , we have measured the effects on catalase activity after adding NADPH either to acellular extracts or during cell culture of the different cell types . - pubmed
Our results show a clear relationship between low catalase activity and striking decrease in intracellular NADPH levels . - pubmed
Hopefully new agents such as lazaroids , endothelins inhibitors and nitric oxyde modulators will become available for clinical use in the next future . - pubmed
BTK was activated in a dose- and time - dependent manner by treatment with the tyrosine phosphatase inhibitor pervanadate . - pubmed
NAD +- dependent 15 - hydroxy - PG dehydrogenase ( PGDH ) is the major enzyme involved in the initial inactivation of PGs , and its activity is reduced by glucocorticoids , cortisol ( F ) , and dexamethasone ( DEX ) . - pubmed
In the placenta , 11beta - HSD2 is the dominant isoform , acting as a dehydrogenase [ F to cortisone ( E ) ] ; and in chorion , 11beta - HSD1 predominates as a reductase ( E to F ) . - pubmed
CBX alone had no effect on PGDH activity in either placenta or chorion trophoblast cells . - pubmed
In chorion , E significantly inhibited PGDH activity , and this effect was reversed by addition of CBX . - pubmed
F and DEX significantly inhibited PGDH , and this effect was unaltered by coadministration of CBX . - pubmed
In contrast , in placenta , there was no effect of E , or of E with CBX , on PGDH activity . - pubmed
However , F and DEX inhibited PGDH , and the effect of F ( but not DEX ) was greater in the presence of CBX . - pubmed
When data from the Neoral and Sandimmune groups were combined , measurements of C0 but not C2 or C6 were associated with the risk of acute lung allograft rejection . - pubmed
A chimeric form of osteoprotegerin inhibits hypercalcemia and bone resorption induced by IL-1beta , TNF - alpha , PTH , PTHrP , and 1 , 25 ( OH ) 2D3 . - pubmed
BDNF and its tyrosine kinase receptor , TrkB , are expressed in hypothalamic nuclei associated with satiety and locomotor activity . - pubmed
A 17 year old girl with active Crohn ' s colitis developed idiopathic thrombocytopenic purpura that was managed with intravenous immune globulins and cyclosporin A. - pubmed
Phospholipase A2 , which is linked to a protein kinase C pathway , hydrolyzes phosphatidylcholine into cis - unsaturated free fatty acids and lysophosphatidylcholine ( lysoPC ) . - pubmed
The results of the present study , thus , demonstrate that phospholipase A2 - linked free fatty acids and lysoPC are employed in the sustained facilitation of hippocampal neurotransmission , suggesting a significant role of a phospholipase A2 signaling pathway in the neuroplasticity . - pubmed
Myeloperoxidase ( MPO ) has been involved in the pathogenesis of several diseases through excessive production of reactive oxygen species ( ROS ) as well as through its genetic polymorphism . - pubmed
The factors affecting MPO levels were age , the number of white cells , smoking in fathers and oral contraceptive intake in mothers . - pubmed
The putative plasma membrane Na (+)/ H (+ ) antiporter SOS1 controls long - distance Na (+ ) transport in plants . - pubmed
In this study , we examined the tissue - specific pattern of gene expression as well as the Na (+ ) transport activity and subcellular localization of SOS1 . - pubmed
Under mild salt stress ( 25 mM NaCl ) , sos1 mutant shoot accumulated less Na(+ ) than did the wild - type shoot . - pubmed
However , under severe salt stress ( 100 mM NaCl ) , sos1 mutant plants accumulated more Na(+ ) than did the wild type . - pubmed
There also was greater Na (+ ) content in the xylem sap of sos1 mutant plants exposed to 100 mM NaCl . - pubmed
These results suggest that SOS1 is critical for controlling long - distance Na (+ ) transport from root to shoot . - pubmed
We present a model in which SOS1 functions in retrieving Na (+ ) from the xylem stream under severe salt stress , whereas under mild salt stress it may function in loading Na (+ ) into the xylem . - pubmed
Statins and low - density lipoprotein cholesterol levels . - pubmed
In embryonic fibroblasts , epidermal growth factor and 12 - O - tetradecanoylphorbol-13 - acetate activated the MAPK cascade to a similar extent , yet only c - Raf-1 activation by epidermal growth factor depended on KSR1 . - pubmed
RECOMBINATION OF PROTOPORPHYRINOGEN WITH CYTOCHROME C APOPROTEIN . - pubmed
Analysis of conformational properties of the chimeric protein showed that all six conformational mAbs interacted with the recombinant protein were reduction - sensitive , indicating that fusion of the two cysteine - rich proteins retains critical conformational epitopes . - pubmed
It was shown that the inhibition is dependent on the presence of Abs to the chimeric protein and their disulfide bond - dependent conformations . - pubmed
Part II : surface - enhanced Raman spectroscopy investigation of methionine containing heterodipeptides adsorbed on colloidal silver . - pubmed
Glutaric aciduria type I and kynurenine pathway metabolites : a modified hypothesis . - pubmed
Deficiency of glutaryl - CoA dehydrogenase blocking the glutarate pathway and activation of indoleamine 2,3 - dioxygenase in macrophages / monocytes by intercurrent inflammation may increase flux down the kynurenine pathway towards the production of quinolinic acid . - pubmed
Quinolinic acid is neurotoxic and is an endogenous agonist at N - methyl - D - aspartate receptors . - pubmed
The two Cr ( tfa ) 3 isomers extracted can be efficiently separated by a DB-210 GC column within 9 min . - pubmed
This depression was partially reversed by 8 - cyclopentlyl-1,3 - dimethylxanthine ( CPT ) , a selective A1 adenosine receptor antagonist . - pubmed
Accumulation of extracellular adenosine and inhibition of adenosine kinase activity were caused by hypercapnic acidosis and isohydric hypercapnia , but not isohydric acidosis . - pubmed
The inhibition of adenosine kinase activity is suggested to cause the accumulation of extracellular adenosine during hypercapnia . - pubmed
A laser - ablation molecular - beam Fourier transform microwave ( LA - MB - FTMW ) spectrometer has been successfully applied to the structural study of alpha - aminobutyric acid . - pubmed
It was found that the former assimilated formaldehyde via the serine pathway whereas the latter did so via the ribulose - monophosphate pathway from the measurement of the key enzyme activities in each assimilation pathway . - pubmed
We report the identification and characterization by thin - layer chromatography ( TLC ) of N - acyl homoserine lactone ( HSL ) quorum sensing signal molecules by PGP Burkholderia . - pubmed
strain CBMB40 produced an additional molecule that migrated along decanoyl ( C(10)- ) HSL . - pubmed
The catalyzed signal amplification ( CSA ) technique , based on the peroxidase - mediated deposition of haptenized tyramide and also known as tyramide signal amplification and catalyzed reportor deposition systems , is widely accepted as a signal amplification method for immunohistochemistry and in situ hybridization . - pubmed
In this study , we examined the applicability of a new simplified CSA system employing fluorescyl - tyramide ( FT ) to pathologic testing and research with formalin - fixed , paraffin - embedded tissues . - pubmed
By using the FT , instead of biotinyl - tyramide ( BT ) that is commonly employed in the CSA system with chromogen , nonspecific staining caused by endogenous biotin was completely avoided . - pubmed
Although Kir4.1 channels are the major inwardly rectifying channels in glial cells and are widely accepted to support K +- and glutamate - uptake in the nervous system , the properties of Kir4.1 channels during vital changes of K + and polyamines remain poorly understood . - pubmed
The heterodimer of the ecdysone receptor ( EcR ) and ultraspiracle ( Usp ) , members of the nuclear receptor superfamily , is considered to be functional receptor for the ecdysteroids that coordinate essential biological processes in insects . - pubmed
Ischemia / reperfusion injury resulted in a decrease in the expression of thioredoxin , while tetramethylpyrazine administration greatly elevated the expression of thioredoxin-1 / thioredoxin-2 mRNA and thioredoxin reductase-1 / thioredoxin reductase-2 mRNA . - pubmed
These findings suggest that administration of tetramethylpyrazine , within a 4h time period post - transient focal stroke , may reduce cerebral ischemic reperfusion damage . - pubmed
Moreover , the neuroprotective effect of tetramethylpyrazine may be mediated , in part , by an increase in genetic transcription of thioredoxin . - pubmed
RESULTS : Elementary modes analysis ( EMA ) of a set of enzymes corresponding to the Krebs cycle , glycolysis and gluconeogenesis supports the scientific evidence showing that there is no pathway capable of converting acetyl - CoA to glucose at steady state . - pubmed
This conversion is possible after the addition of isocitrate lyase and malate synthase ( forming the glyoxylate shunt ) to the system . - pubmed
Reducing the risk of prostate cancer has gained increasing coverage in recent years , with proof of principle shown in the Prostate Cancer Prevention Trial with the type 2 5alpha - reductase ( 5AR ) inhibitor , finasteride . - pubmed
Several agents are under investigation for reducing the risk of prostate cancer , including selenium / vitamin E and selective oestrogen receptors modulators ( e.g. - pubmed
In addition , the Reduction by Dutasteride of Prostate Cancer Events trial , involving & gt;8000 men , is evaluating the effect of the dual 5AR inhibitor , dutasteride , on the risk of developing prostate cancer . - pubmed
PURPOSE : Vandetanib is a once - daily oral inhibitor of vascular endothelial growth factor receptor ( VEGFR ) and epidermal growth factor receptor ( EGFR ) signaling . - pubmed
In this two - part phase II study , the efficacy and safety of vandetanib was compared with that of gefitinib , an inhibitor of EGFR signaling . - pubmed
AIM : Heparanase , a glycohydrolase enzyme , cleaves heparan sulfate in the tissue matrix . - pubmed
On further investigation he had grade II hepatocellular iron staining and a hepatic index of 5.4 leading to a diagnosis of hereditary hemochromatosis . - pubmed
Exogenous soluble VEGF receptor-1 ( sFlt-1 ) regulates Th1 / Th2 cytokine production from normal placental explants via intracellular calcium . - pubmed
The aim of this study is to examine if exogenous sFlt-1 can regulate Th1 / Th2 cytokines IL-10 and TNF - alpha production from normal placental explants via intracellular calcium release . - pubmed
Intracellular calcium blocker , 1,2 - bis -( o - aminophenoxy )- ethane - N , N , N ' , N '- tetraacetic acid , tetra ( acetoxymethyl )- ester ( BAPTA / AM ) was applied to investigate whether the changes in cytokine concentration were mediated by intracellular free calcium . - pubmed
The increase in IL-10 can be inhibited by BAPTA / AM . - pubmed
The change in Th1 / Th2 cytokine level may be mediated by intracellular free calcium . - pubmed
Moreover , the co - purified catalytic domain plus site - directed mutants of the D123 protein lacking these aromatic residues showed that W366 was key to the apparent affinity for the polysaccharide substrate of starch synthase III , whereas either of these amino acid residues altered ADP - glucose kinetics . - pubmed
The sensitivity of the direct wet smear was 45.8 % , that of the prolonged May - Grunwald Giemsa staining was 58.3 % , and that of the SAF / methylene blue method was 62.5 % . - pubmed
Both ATP and adenosine suppress cardiac pacemakers ' automaticity and atrioventricular nodal conduction , albeit via the different mechanism of actions . - pubmed
Canonical Wnt3a modulates intracellular calcium and enhances excitatory neurotransmission in hippocampal neurons . - pubmed
Our results identify the Wnt3a protein and a member of its complex receptor at the membrane , the low density lipoprotein receptor - related protein 6 ( LRP6 ) coreceptor , as key molecules in neurotransmission modulation and suggest cross - talk between canonical and Wnt / Ca ( 2 + ) signaling in central neurons . - pubmed
Three sterically crowded peri - substituted naphthalene phosphines , Nap [ PPh ( 2 )][ ER ] ( Nap = naphthalene-1,8 - diyl ; ER = SEt , SPh , SePh ) 1 - 3 , which contain phosphorus and chalcogen functional groups at the peri positions have been prepared . - pubmed
M and further pharmacological experiments suggested that the receptors involved in this inhibitory effect are of the H ( 3 ) type . - pubmed
Also in view of data reported by others it is suggested tentatively that in general presynaptic modulation of neurotransmitter release by histamine , if it occurs , involves activation of H ( 3 ) receptors . - pubmed
Immunoblotting and immunohistochemistry were used to determine the phospho - Akt ( pAkt Ser473 ) , phospho - eNOS ( peNOS Ser1177 ) , and eNOS levels and immunofluorescence to determine the cellular distribution of eNOS and pAkt Ser473 . - pubmed
Wortmannin treatment before LPS preconditioning significantly reduced eNOS expression in neurons and endothelial cells , which inhibited LPS - induced protection against HI . - pubmed
Phosphatidylinositol 5 - phosphate links dehydration stress to the activity of ARABIDOPSIS TRITHORAX - LIKE factor ATX1 . - pubmed
Interestingly , ATX1 contains a PtdIns5P interaction domain ( PHD finger ) and thus , phosphoinositide signaling , may link environmental stress to changes in gene transcription . - pubmed
HVOD occurred during chemotherapy protocols of CAM ( CTX + Ara - C + 6 - TG ) or maintenance period ( MTX + 6 - TG ) . - pubmed
Predicting the outcome of grade II glioma treated with temozolomide using proton magnetic resonance spectroscopy . - pubmed
BACKGROUND : This study was designed to evaluate proton magnetic resonance spectroscopy ( ( 1 ) H - MRS ) for monitoring the WHO grade II glioma ( low - grade glioma ( LGG ) ) treated with temozolomide ( TMZ ) . - pubmed
Response to TMZ was evaluated by monthly MRI examinations that included MRI with volumetric calculations and ( 1 ) H - MRS for assessing Cho / Cr and Cho / NAA ratios . - pubmed
Thus , ( 1 ) H - MRS may be a promising , non - invasive tool for predicting and monitoring the clinical response to TMZ . - pubmed
Among the 10 DNA polymerase mutations selected with ganciclovir , 7 ( P488R , C539R , L545S , V787L , V812L , P829S , and L862F ) were associated with ganciclovir resistance , whereas 2 ( F595I and V946L ) conferred only foscarnet resistance . - pubmed
Moreover , compared with KCl and NE , ANG II contractions were diminished & gt;= 5 - fold . - pubmed
Whereas KCl and ANG II contracted third generation>>precaruncular / placental UVSM , NE - induced contractions were similar throughout the UVB . - pubmed
Expanded polytetrafluoroethylene ( ePTFE ) is unique in that it can be visualized on computed tomography ( CT ) . - pubmed
Our findings suggest that CR activates mitochondrial energy metabolism and fatty acid biosynthesis in WAT . - pubmed
In contrast , in BAT CR either had no effect on , or down - regulated , the mitochondrial electron transport chain , but enhanced fatty acid biosynthesis . - pubmed
O - GlcNAcylation is an unusual form of protein glycosylation , where a single - sugar [ GlcNAc ( N - acetylglucosamine ) ] is added ( via beta - attachment ) to the hydroxyl moiety of serine and threonine residues of nuclear and cytoplasmic proteins . - pubmed
We have previously described a novel mGlu5 positive allosteric modulator ( PAM ) , LSN2463359 and provided evidence of its ability to attenuate aspects of the behavioural response to administration of the competitive NMDA receptor antagonist , SDZ 220,581 . - pubmed
In the present study , the interactions between this mGlu5 PAM and the NMDA receptor were explored further by assessing the effects of LSN2463359 against some of the motor , instrumental and cognitive effects induced by the non - competitive NMDA receptor antagonists PCP and MK-801 , the competitive NMDA receptor antagonist SDZ 220,581 and the GluN2B selective NMDA receptor antagonist , Ro 63 - 1908 . - pubmed
Of several chemical chaperones tested , the addition of betaine resulted in increased secretion of FVIII , by increasing solubility of intracellular FVIII aggregates and improving transport from endoplasmic reticulum to Golgi . - pubmed
Moreover , in vitro and in vivo applications of betaine were also able to rescue a trafficking - defective FVIII mutant ( FVIIIQ305P ) . - pubmed
In addition , megakaryocyte - specific cell surface markers showed a dramatic increase in expression in response to ( R )- TEMOSPho treatment . - pubmed
We detected caspase - dependent cleavage substrates by quantifying protein intensities before and after TRAIL induction in SDS gel slices . - pubmed
RESULTS : the ICER of the adding - on regimens range from negative values for Gabapentin , Levetiracetam and Zonisamide to low values for Lamotrigine ( ~ 6.4 million Rials / consequence [ mil Rls / consq ] ) and Oxcarbazepine ( ~7.7 mil Rls / consq ) and followed by high values for Topiramate ( ~21 mil Rls / consq ) and Vigabatrin ( ~43.7 mil Rls / consq ) considering the three months of remaining on regimen . - pubmed
The results showed that KP - SMEDDS and KP-2 - HP - beta - CD complex improved the dissolution rate of methoxyflavones in both 0.1N HCl and 0.2 M PBS pH 6.8 compared to KP dissolved in a solution of propylene glycol , PEG 400 , ethanol , and water . - pubmed
The oral bioavailability values of KP - SMEDDS formulations were higher than those of KP ( 25.38- , 42.00- , and 26.01 - fold for PMF , TMF , and DMF , respectively ) . - pubmed
Zinc as mediator of ubiquitin conjugation following traumatic brain injury . - pubmed
Here we assessed the effect of zinc on ubiquitin conjugation and subsequent neurodegeration following traumatic brain injury ( TBI ) . - pubmed
Our previous study has shown that zinc can induce ubiquitin conjugation in cultured hippocampal neurons . - pubmed
RESULTS : In the ISOR sample , APOE epsilon4 allele carriers had higher plasma triglyceride ( p=0.0002 ) , total cholesterol ( p=0.009 ) and LDL - cholesterol ( p=0.003 ) levels than epsilon3 allele carriers . - pubmed
Linkage disequilibrium and haplotype analyses confirmed the respectively deleterious and protective impacts of the epsilon4 and epsilon2 alleles on LDL - cholesterol levels and showed that the G allele of the rs4420638 polymorphism may exert a protective effect on LDL - cholesterol levels in subjects bearing the APOE epsilon 4 allele . - pubmed
CONCLUSION : Our results showed that ( i ) the APOE epsilon polymorphism has the expected impact on the plasma lipid profile and ( ii ) the rs4420638 G allele may counterbalance the deleterious effect of the epsilon4 allele on LDL - cholesterol levels in an Algerian population . - pubmed
The potassium chloride cotransporters ( KCC ) family of proteins are widely expressed and are involved in the transepithelial movement of potassium and chloride ions and the regulation of cell volume . - pubmed
RESULTS : While the maximum specific growth rates of the mutants and the parental strain were similar at both temperatures ( 0.33 + - 0.02 h(-1 ) and 0.34 + - 0.03 h(-1 ) , respectively ) , DNA microarray analyses revealed that the CtsR or HrcA deficient strains displayed altered transcription patterns of genes encoding functions involved in transport and binding of sugars and other compounds , primary metabolism , transcription regulation , capsular polysaccharide biosynthesis , as well as fatty acid metabolism . - pubmed
The ANP group ( n = 40 ) was injected with 4.5 % sodium taurocholate into the pancreatic duct to induce ANP . - pubmed
RESULTS : IAP showed a continuous upward trend in the ANP group , with values 2 to 3 times higher than those in the SO group at the corresponding time points and the rising rate was peaking at 6 h. The levels of plasma amylase , TNF - alpha , Cr , TB , DAO , and D - lactate also gradually increased in the ANP group over time and were significantly higher than in the SO group at 3 , 6 , 12 and 24 h ( all P<0.05 ) . - pubmed
The protocol also allows the one - pot transformation of aromatic nitro compounds into dimethylated amines in the presence of a simple iron catalyst . - pubmed
We have demonstrated that deleting a dispensable gene involved in galactose metabolism ( ugeA ) and one involved in biosynthesis of a minor cell wall component ( ugmA ) led to changes in hyphal elemental distribution that may have resulted from compromised wall composition . - pubmed
RESULTS : In the probability of target attainment ( PTA ) analyses , only 2 g of iv vancomycin every 24 h conferred & gt;90 % probability of achieving an AUC / MIC ratio of & gt;400 for MIC values & lt;2 mg / L in the serum . - pubmed
CVD risk factors included diabetes ( self - reported diagnosis , glycated hemoglobin & gt;=6.5 % , or fasting plasma glucose & gt;=126mg / dl ) , hypertension ( treatment or blood pressure & gt;=140/90 mmHg ) , dyslipidemia ( treatment or non - HDL cholesterol & gt;=160 mg / dl ) , and smoking ( self - report or cotinine levels & gt;=10 ng / ml ) . - pubmed
The short - term incorporation of 13C ( H13CO3 ) in phytoplankton and bacterial lipid biomarkers was measured as well as the total bacterial carbon production ( BP ) , viral lysis and the microbial community structure under three experimental conditions : ( 1 ) High inorganic Nutrient and High Grazing ( HN + HG ) , ( 2 ) High inorganic Nutrient and Low Grazing ( HN + LG ) and ( 3 ) under natural in situ conditions with Low inorganic Nutrient and High Grazing ( LN + HG ) during spring . - pubmed
Theanine improves the function of dendritic cells via the downregulation of cyclooxygenase-2 expression . - pubmed
We sought to measure cyclooxygenase-2 ( COX-2 ) expression in bombesin - inhibited DCs treated with theanine in vitro and to explore the protection and activation effects of theanine on DCs . - pubmed
METHODS : Enzyme - linked immunosorbent assay ( ELISA ) , reverse transcription - polymerase chain reaction ( RT - PCR ) , and Western blotting were used to analyze the effects of theanine on COX-2 expression and interleukin ( IL )- 12 / IL-10 secretion of bombesin - treated DCs . - pubmed
Theanine increased the expression of mature DC surface molecules . - pubmed
COX-2 expression in bombesin - treated DCs was inhibited by theanine in a dose - dependent manner . - pubmed
Theanine promoted DC secretion of IL-12 . - pubmed
IL-12 levels reached ( 137.4 + - 4.9 ) pg / ml with theanine at 200 micromol / L. However , theanine inhibited the secretion of IL-10 in a dose - dependent manner . - pubmed
IL-10 levels were only ( 58.4 + - 6.9 ) pg / ml with theanine at 200 micromol / L. CONCLUSION : Theanine inhibits the transcription and translation of COX-2 and regulates the balance of IL-10 / IL-12 secretion in bombesin - inhibited DCs , leading to the recovery of a state of activation in DCs . - pubmed
Dynamic and coordinated regulation of KEAP1 - NRF2 - ARE and p53 / p21 signaling pathways is associated with acetaminophen injury responsive liver regeneration . - pubmed
The expression and nuclear accumulation of NRF2 was increased after APAP treatment . - pubmed
The content of p53 and its downstream target p21 were significantly increased upon APAP exposure and subsequently decreased to normal levels at 48 hours . - pubmed
These results demonstrated that dynamic and coordinated regulation of KEAP1 - NRF2 - ARE and p53 / p21 signaling pathways was associated with compensatory liver regeneration after APAP - induced acute liver injury . - pubmed
RECENT FINDINGS : Since the landmark discovery that bone morphogenetic protein receptor type II ( BMPR2 ) mutations cause the majority of cases of familial PAH , investigators have discovered mutations in genes that cause PAH in families without BMPR2 mutations , including the type I receptor ACVRL1 and the type III receptor ENG ( both associated with hereditary hemorrhagic telangiectasia ) , caveolin-1 ( CAV1 ) , and a gene ( KCNK3 ) encoding a two - pore potassium channel . - pubmed
We describe the use of LC - MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light - directed in situ chemistry . - pubmed
The aim of this work was to evaluate the influence of the adsorption process with granular activated carbon ( GAC ) on the chlorine dioxide consumption in final oxidation / disinfection . - pubmed
Moreover , a good correlation was found between the ClO2 - D and UV absorbance at wavelength 254 nm using mineral carbons ; therefore , the use of a mineral mesoporous GAC is an effective solution to control the high ClO2 - D in the disinfection stage of a DWTP . - pubmed
Cocrystal structures of central region of PfAMA1 containing disulfide - linked clusters ( domains I and II ) with R1 peptide and a peptide derived from PfRON2 showed strong structural similarity in binding . - pubmed
The cross - linking capacity of homodimeric lectins was enhanced by covalent connection of the two carbohydrate - binding sites . - pubmed
The bone mineral density ( BMD ) of the lumbar vertebra and hip joint , and the level of serum bone gla protein ( S - BGP ) and hydroxyproline / creatinine ( Hyp / Cr ) were observed before and after treatment in the two groups . - pubmed
( 2)After treatment , the index of bone metabolism in the control group was increased , and the serum S - BGP , the Hyp / Cr in the control group were higher than those in the observation group ( both P<0 . - pubmed
plantarum maltose - producing alpha - amylase ( LpMA ) belongs to glycoside hydrolase family 13 . - pubmed
It produced only G2 from the hydrolysis of various substrates , including maltotriose ( G3 ) , maltopentaose ( G5 ) , maltosyl beta - cyclodextrin ( G2 - beta - CD ) , amylose , amylopectin , and starch . - pubmed
Reaction pattern analysis using 4 - nitrophenyl - alpha - d - maltopentaoside ( pNPG5 ) demonstrated that LpMA hydrolyzed pNPG5 from the nonreducing end , indicating that LpMA is an exotype alpha - amylase . - pubmed
Kinetic analysis revealed that LpMA had the highest catalytic efficiency ( kcat / Km ratio ) toward G2 - beta - CD . - pubmed
BAEO was isolated by hydrodistillation , and gas chromatography - mass spectrometry ( GC - MS ) analysis identified benzaldehyde ( 75.35 % ) , benzoic acid ( 6.21 % ) and mandelonitrile ( 5.38 % ) . - pubmed
Z - VAD - FMK , a broad - spectrum caspase inhibitor , attenuated BAEO - induced apoptosis . - pubmed
Also , increased Bax and decreased Bcl-2 levels suggest that BAEO - induced apoptosis is mediated through both death receptor and mitochondrial pathways . - pubmed
Moreover , reduced Rel / NF - kappaB levels suggest that BAEO - mediated apoptosis is also associated with inhibition of the NF - kappaB pathway . - pubmed
NAC was diluted in MHB to a starting concentration of 160 mg / mL and serial two - fold microdilution assays were performed in triplicate with negative controls for all isolates tested . - pubmed
RESULTS : There were significant reduction in the levels of the interleukin-6 and cardiac troponin - I in the trimetazidine - treated group ( group 2 ) compared to the control group ( group 1 ) ( P<0.001 ) , after 12h and 24h post - operative . - pubmed
beta - Catenin signaling positively regulates glutamate uptake and metabolism in astrocytes . - pubmed
CONCLUSIONS : These studies confirm that beta - catenin regulates key proteins responsible for excitatory glutamate neurotransmission in vitro and in vivo and reveal the therapeutic potential of beta - catenin modulation in treating diseases with abnormal glutamatergic neurotransmission and excitotoxicity . - pubmed
Purpose : The purpose of this study was to evaluate the antitumor efficacy of the reduced immunogenicity anti - mesothelin immunotoxin RG7787 plus nab - paclitaxel against primary mesothelioma cell lines and tumor xenografts and the utility of mesothelin as a biomarker of tumor response . - pubmed
Conclusions : RG7787 plus nab - paclitaxel is very active against primary human mesothelioma cells in vitro as well as in vivo , with serum mesothelin levels correlating with tumor response . - pubmed
To induce neuroprogenitor cells , EBs were cultured in the presence of retinoic acid , N2 supplement and fibroblast growth factor followed by culturing in a neurobasal medium containing B27 , N2 supplements for additional 10 days , to allow the maturation and development of neuronal progenitor cells . - pubmed
In particular , the tumor microenvironment includes elevated levels of ATP required for activation of P2X7R and the sustained tumor and immune cell P2X7R - mediated responses which in total contribute to overall tumor growth and viability . - pubmed
Supplementation of RLP significantly decreased semen Malondialdehyde ( MDA ) concentration . - pubmed
Androgen Triggers the Pro - Migratory CXCL12 / CXCR4 Axis in AR - Positive Breast Cancer Cell Lines : Underlying Mechanism and Possible Implications for the Use of Aromatase Inhibitors in Breast Cancer . - pubmed
We have shown that androgen induces C - X - C motif chemokine 12 ( CXCL12 ) in BC cell lines . - pubmed
Our aim was to identify the mechanisms regulating the phenotypic effects of androgen - induced CXCL12 on Androgen Receptor ( AR ) positive BC cell lines . - pubmed
The steroid receptor co - regulator NCOA1 is critical for this effect . - pubmed
A synbiotic - containing amino - acid - based formula improves gut microbiota in non - IgE - mediated allergic infants . - pubmed
Annexin V / PI and cell cycle analysis showed the maximum apoptosis - inducing effect of the dichloromethane fraction at 200 mug / mL. - pubmed
The Effect of the Oral Administration of Leucine on Endothelial Function , Glucose and Insulin Concentrations in Healthy Subjects . - pubmed
Serum glucose and insulin were measured at baseline and every 10 min after glucose infusion for 1 h. Endothelial function was evaluated by measurement of flow mediated vasodilatation ( FMD ) at baseline , 10 and 60 min after glucose infusion . - pubmed
RESULTS : In both visits , glucose levels increased to the same degree , whereas insulin response was significantly higher after leucine administration . - pubmed
Leucine administration prevents hyperglycaemia - mediated endothelial dysfunction probably due to enhanced insulin secretion . - pubmed
Previous studies have found that renin - angiotensin - aldosterone system ( RAAS ) played vital and bidirectional roles in ALI . - pubmed
The expression levels of angiotensinII ( AngII ) and angiotensin -( 1 - 7 ) ( Ang -( 1 - 7 ) ) were measured through ELISA . - pubmed
There were notable seasonal changes in PAH concentrations : winter & gt ; autumn & gt ; spring & gt ; summer . - pubmed
CONCLUSIONS : For teeth restored with adhesive procedures and lithium disilicate onlays , the presence of build - up with fiber post to provide retention and resistance form does not influence the fracture strength . - pubmed
In the present study , using GFP - ATG8 gene as a marker for tracking putative autophagosomes , we confirmed that autophagic vacuolization may lead to autophagic cell death in the DBA - treated parasites . - pubmed
Saliva samples were plated in Mannitol salt , and isolates were identified by DnaJ gene sequencing . - pubmed
But we blaze a trail by amalgamating the pharmacological mechanistic studies of KYNA in kinship with alpha7nAChRs , NMDARs and GABA which lends aid in favour of KA . - pubmed
We report a crystal structure of this divergent Cas6 , identify amino acids required for Cas6 activity , show that the Cas6 domain is required for RT activity and RNA spacer acquisition , and demonstrate that CRISPR - repeat binding to Cas6 regulates RT activity . - pubmed
But none of the treatments either in in vitro or ex vivo cardiac models , induced a modulation of desmin , phosphorylated and O - GlcNAcylated desmin expression , despite the presence of O - GlcNAc moities in cardiac desmin . - pubmed
Here we design and synthesize two D - A polymers which both carry a fluorinated quinoxaline acceptor unit , but while P1 includes a benzodithiophene donor moiety with thiophene side - groups ( 2D - BDT ) , the P2 polymer lacks 2D conjugation in its simpler pentathiophene donor segment . - pubmed
However , poor fill factors limit the preliminary power conversion efficiencies to 3.3 % for P1 and 1.0 % for P2 as blended with PCBM[70 ] in a 1 : 1.5 ( w / w ) ratio . - pubmed
UH3 - 1 , a newly isolated anaerobic gut fungus , grows on diverse untreated substrates through production of a broad range of carbohydrate active enzymes that are robust to variations in substrate composition . - pubmed
Neither tacrolimus ( OR , 0.75 ; 95 % CI , 0.47 - 1.21 ; p = 0.24 ) nor cyclosporine ( OR , 1.40 ; 95 % CI , 0.89 - 2.18 ; p = 0.14 ) were related significantly with post - LTx obesity . - pubmed
One of the most common mechanisms attributed to spectral shifts in FP is excited state proton transfer ( ESPT ) , hydroxyl moiety protonation and deprotonation , along with chromophore cis - trans isomerism . - pubmed
Tamoxifen 's interference potential may be countered by using higher iCR doses or iCR + HP . - pubmed
The present study demonstrated that cyclic adenosine monophosphate signaling in a wounded cell was induced by autocrine purinergic signaling , and protein kinase A potentiates membrane resealing for repeated wounds in those cells . - pubmed
A new coordination polymer ( CP ) , namely poly [( mu-4,4 '- bipyridine )( mu3 - 3,4 '- oxydibenzoato ) cobalt ( II ) ] , [ Co ( C14H8O5 )( C10H8N2 )] n or [ Co ( 3,4 '- obb )( 4,4 '- bipy )] n ( 1 ) , was prepared by the self - assembly of Co ( NO3 ) 2 6H2O with the rarely used 3,4 '- oxydibenzoic acid ( 3,4 '- obbH2 ) ligand and 4,4 '- bipyridine ( 4,4 '- bipy ) under solvothermal conditions , and has been structurally characterized by elemental analysis , IR spectroscopy , single - crystal X - ray crystallography and powder X - ray diffraction ( PXRD ) . - pubmed
Circulating levels of glucose , prolactin , testosterone , gonadotropins , thyrotropin , and free thyroid hormones were measured , while the structure parameters of thyroid homeostasis and the degree of insulin sensitivity were calculated at baseline and 16 weeks later . - pubmed
In both study groups , metformin decreased plasma glucose levels and improved insulin sensitivity . - pubmed
Metformin - induced changes in thyrotropin and Jostel 's index correlated with their baseline values , baseline levels of testosterone , and with the effect of treatment on insulin sensitivity . - pubmed
Redox regulation of hepatic NLRP3 inflammasome activation and immune dysregulation in trichloroethene - mediated autoimmunity . - pubmed
In addition , TCE exposure led to prominent lobular inflammation with sinusoid dilation , increased sinusoidal cellularity and increased staining for proliferating cell nuclear antigen ( PCNA ) , confirming inflammatory and hepatocellular cell proliferation . - pubmed
Importantly , TCE exposure resulted in the activation of hepatic inflammasome ( NLRP3 and caspase-1 ) and up - regulation of pro - inflammatory cytokine IL-1beta , and these changes were attenuated by NAC supplementation . - pubmed
Antileukemic Efficacy in Vitro of Talazoparib and APE1 Inhibitor III Combined with Decitabine in Myeloid Malignancies . - pubmed
Here , we investigated the cytotoxic efficacy of talazoparib and APE1 inhibitor III , inhibitors of PARP1 / 2 and APE1 , in primary CD34 + MDS / CMML cell samples ( n = 8 ; 4 MDS and 4 CMML ) and in primary CD34 + or CD34- AML cell samples ( n = 18 ) in comparison to healthy CD34 + donor cell samples ( n = 8) . - pubmed
Strikingly , talazoparib and APE1 inhibitor III demonstrated critical antileukemic efficacy in selected MDS / CMML and AML cell samples . - pubmed
Low doses of talazoparib and APE1 inhibitor III further increased the cytotoxic efficacy of decitabine in MDS / CMML and AML cells . - pubmed
Moreover , low doses of APE1 inhibitor III increased the cytotoxic efficacy of talazoparib in MDS / CMML and AML cells . - pubmed
In summary , talazoparib and APE1 inhibitor III demonstrated substantial antileukemic efficacy as single agents , in combination with decitabine , and combined with each other . - pubmed
Nonetheless , T2 * -based methods become challenging when fat and iron are simultaneously present . - pubmed
This study proposes a phantom design concomitantly containing various concentrations of iron and fat suitable for devising accurate simultaneous T2 * and fat quantification technique . - pubmed
MATERIALS AND METHODS : A 46 - vial iron - fat - water phantom with various iron concentrations covering clinically relevant T2 * relaxation time values , from healthy to severely overloaded liver and wide fat percentages ranges from 0 to 100 % was prepared . - pubmed
T2 * relaxation times and fat fractions were extracted from the MR signals to explore the effects of fat and iron overload . - pubmed
Therefore , T2 * values from the mono - exponential signal equation were used and expressed the relationship between relaxation time value across all iron ( Fe ) and fat concentration as [ Formula : see text ] , with R - squared = 0.89 . - pubmed
DISCUSSION : The proposed phantom design with microsphere iron particles closely simulated the single - T2 * behavior of fatty iron - overloaded liver in vivo . - pubmed
Cetuximab enhances the efficiency of irinotecan through simultaneously inhibiting the MAPK signaling and ABCG2 in colorectal cancer cells . - pubmed
RESULTS : Cetuximab was found to increase intracellular concentrations of irinotecan as well as cytotoxicity by inhibiting the epidermal growth factor receptor and , by extension , the downstream RAS - RAF - MEK - ERK signaling pathway . - pubmed
It was also shown that cetuximab inhibited the drug efflux activity of ABCG2 . - pubmed
In combination with irinotecan , cetuximab can both significantly induce cell apoptosis by inhibiting the RAS - RAF - MEK - ERK signaling pathway and improve the effects of irinotecan by decreasing drug efflux through the inhibition of ABCG2 . - pubmed
Terrestrial Fluxes of Carbon in GCP Carbon Budgets . - pubmed
The Global Carbon Project ( GCP ) has published global carbon budgets annually since 2007 ( Raupach et al . - pubmed
The GCP budget is composed of annual perturbations from pre - industrial conditions , driven by additions of carbon to the system from combustion of fossil fuels and by transfers of carbon from land to the atmosphere as a result of land use . - pubmed
Although the GCP budgets have focused on the two net terrestrial fluxes , they have paid little attention to the gross components , which are important for a number of reasons , including understanding the potential for land management to remove CO2 from the atmosphere and understanding the processes responsible for the sink for carbon on land . - pubmed
In this study , analytical techniques were combined with enzymatic assays to develop a novel method for the detection of snake venom protease activity by using rhodamine-110 - peptide substrate . - pubmed
The application of the rhodamine-110 - peptide substrate assay showed significantly greater sensitivity compared to prior assays using casein - FITC as the substrate . - pubmed
Nicotine - induced degradation of elastin and collagen fibers were significantly suppressed in BN group . - pubmed
These results suggest that proanthocyanidins - rich BSSCE can prevent the weakening of the aortic wall via inhibiting MMP-2 upregulation . - pubmed
Two tyrosine kinase inhibitors , vandetanib and cabozantinib , have been approved for treatment of advanced MTC , but options beyond those are limited . - pubmed
Sulfanylmethyldimethylaminopyridine as a Useful Thiol Additive for Ligation Chemistry in Peptide / Protein Synthesis . - pubmed
The alkyl thiol in 2 allows it to be used for the one - pot native chemical ligation - desulfurization protocol in peptide synthesis . - pubmed
The levodopa and Bushen Zhichan recipe group exhibited decreased glutamate receptors and increased gamma - aminobutyric acid receptors in the striatum . - pubmed
This translocation of TrkB depends on co - factors and modulators of cAMP levels , N - glycosylation , and receptor transactivation . - pubmed
Personalized warfarin treatment based on the PITX2 single nucleotide polymorphism rs6843082 . - pubmed
In therapies targeting EGFR , the development of resistance owing to mutations and single nucleotide polymorphisms , and the expression of the receptor ligands themselves are very serious issues . - pubmed
However , neonatal jaundice ( hyperbilirubinemia ) derived from HO overexpression led to the development of inhibitors , such as those based on metaloproto- and meso - porphyrins inhibitors with competitive activity . - pubmed
In vitro , 10 microM norepinephrine markedly downregulated the expressions of prolactin , IGFBP1 , and PLZF , which are the specifical markers of decidual stromal cells during decidualization . - pubmed
The alpha1b - adrenergic receptor expression was upregulated by norepinephrine . - pubmed
Interestingly , norepinephrine did not inhibit the expression of IGFBP1 in endometrial stromal cells after silencing alpha1b - adrenergic receptor , while significantly suppressed the induced decidualization with overexpression of alpha1b - adrenergic receptor . - pubmed
When alpha1b - adrenergic receptor was activated , endometrial p - PKC was significantly increased under post - treatment with norepinephrine in vivo and in vitro . - pubmed
Therefore , norepinephrine exposure inhibited endometrial decidualization through the activation of the PKC signaling pathway by upregulating alpha1b - adrenergic receptor . - pubmed
While few findings were replicated by multiple strong studies , brain derived neurotrophic factor ( BDNF ) and gamma aminobutyric acid ( GABA ) in depression , BDNF in post - stroke deficits , and beta - endorphin in chronic pain may be altered by rTMS . - pubmed
CONCLUSION : BDNF , GABA and beta - endorphin were identified as potential molecular markers of rTMS and warrant further exploration . - pubmed
RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of cholesterol moved from RBCs to HDL , while cholesterol from LDL moved in the opposite direction . - pubmed
However , specific cholesterol efflux from RBCs to isolated apoA - I was negligible , and ABCA1 silencing with siRNA or inhibition with vanadate and Probucol did not inhibit the efflux to apoA - I , HDL , or plasma . - pubmed
Bioinformatics analysis identified ABCA7 , ABCG5 , lipoprotein lipase , and mitochondrial translocator protein as possible candidates that may mediate the cholesterol flux . - pubmed
Pre - incubation of sections in media containing formaldehyde and glycine or with buffers of different pH , followed by incubation for myosin - ATPase , also differentiated the muscle fibres into a specific distribution pattern within the myotomes , resembling that of the oxidative enzymes to some extent . - pubmed
After 2 - -3 additional days in vitro these aggregates were shown by three different criteria to have developed such a conductance mechanism : ( a ) action potential upstroke velocities greater than 90 V / sec ; ( b ) suppression of spontaneous activity by tetrodotoxin ; and ( c ) presence of a fast component of inward current recorded during voltage clamp . - pubmed
These include hydrolases and nonenzymatic substances ordinarily contained within lysosomes , as well as oxygen - derived free radicals . - pubmed
The method depends on the enzymatic decolourization of the blue dye dichlorophenolindophenol by lactate in the presence of the enzyme L - lactate cytochrome c oxidoreductase ( EC 1.1.2.3 ) . - pubmed
Kinetics of superoxide scavenging by dismutase enzymes and manganese mimics determined by electron spin resonance . - pubmed
Enzymic reactions were studied in which Cu , Zn - SOD , Mn - SOD and Fe - SOD each competed with the spin trap 5,5 - dimethyl-1 - pyrroline 1 - oxide ( DMPO ) for superoxide anion ( O2- ) at pH 7.8 O2- from dissolved KO2 ( potassium superoxide ) in dimethyl sulphoxide was added directly to the enzyme solutions containing DMPO . - pubmed
The results show that , in this competition reaction system , the kinetics of the reactions between the enzymes and O2- follow a function y = f [ ( SOD]0.5 ) . - pubmed
S-1 , determined for Cu , Zn - SOD is approximately an order of magnitude larger than those for Mn - SOD and Fe - SOD . - pubmed
A comparative study of reported SOD mimics , including Mn2 + , MnO2 - desferrioxamine mesylate ( Desferal ) and MnO2 - Desferal - ascorbate , was done . - pubmed
A last generation drug belonging to this family is pravastatin which differs from the other substances inhibiting the activity of the key enzyme of cholesterol metabolism , HMGCaA reductase , because of certain features of the molecule , such as hydrophilia and the fact it is already pharmacologically active at the moment of oral administration . - pubmed
Pravastatin , which is probably a special category of HMGCoA reductase inhibitor , has shown , in numerous experimental studies and controlled clinical trials , a notable effectiveness in reducing in a highly selective fashion the synthesis of cholesterol in the liver cells and consequently the number of cholesterol - rich lipoproteins in the systemic circulation , without also determining significant biologically negative side - effects . - pubmed
The binding of A1 to the arm - like projections of the high ( H)- and medium ( M)-molecular - weight neurofilament subunits causes a conformational change of the molecule ( intrafilamentous reaction ) , characterized by an altered migration on sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( SDS - PAGE ) . - pubmed
The possibility that phosphate groups of the H and M sidearms are involved in binding A1 ions is discussed with regard to the migration on SDS - PAGE of dephosphorylated neurofilaments incubated with A1 compounds and the alteration by A1 ions of neurofilament phosphorylation in vitro by the associated kinase . - pubmed
Hb Coimbra or alpha 2 beta ( 2)99(G1)Asp----Glu , a newly discovered highoxygen affinity variant . - pubmed
We have identified a new high oxygen affinity hemoglobin variant in members of a Portuguese family ; it is characterized by an Asp ---- Glu replacement at codon 99 of the beta chain which is in the alpha 1 beta 2 interface . - pubmed
The altered functional properties of Hb Coimbra likely result from the inability to form a hydrogen bond between beta 99Glu and alpha 42Tyr ; such a bond is formed in deoxy Hb A between the normally occurring beta 99Asp and alpha 42Tyr . - pubmed
A review of the many variants of the alpha and beta chains identifies primarily aspartic acid and glutamic acid residues as being most frequently replaced ; it is speculated that codons GAC and GAT ( for Asp ) , and GAG and GAA ( for Glu ) are most susceptible to mutational events . - pubmed
Previous pKa determinations indicated that histidine 134 , present in the catalytic site of aspartate transcarbamylase , might be the group involved in the binding of the substrate carbamyl phosphate and , possibly , in the catalytic efficiency of this enzyme . - pubmed
The results obtained show that histidine 134 is indeed the group of the enzyme whose deprotonation increases the affinity of the catalytic site for carbamyl phosphate . - pubmed
In the wild - type enzyme this group can be titrated only by those carbamyl phosphate analogues that bear the carbonyl group . - pubmed
In the modified enzyme the group whose deprotonation increases the catalytic efficiency is still present , indicating that this group is not the imidazole ring of histidine 134 ( pKa = 6.3 ) . - pubmed
Out of 22 medullary respiratory neurones consisting of 14 post - inspiratory and 8 expiratory neurones , 12 neurones were depolarized by ethanol and 10 neurones were hyperpolarized . - pubmed
Ethanol depressed the spike activity during stage I expiration of the post - inspiratory neurones . - pubmed
The present results demonstrate that ethanol reduces the expiratory period mainly through the depression of the post - inspiratory neuronal activity in the bulbar respiratory control mechanism . - pubmed
Clinical pharmacology of pinacidil , a prototype for drugs that affect potassium channels . - pubmed
The clinical pharmacology of potassium channel openers has been reviewed using pinacidil as a prototype drug . - pubmed
Plasma renin activity , however , was not increased during chronic therapy with pinacidil monotherapy . - pubmed
, similar efficacy relative to placebo was noted with a change of post - dose supine diastolic blood pressure in the pinacidil group of 13.5 + /- 0.8 mm Hg and 7.3 + /- 0.9 mm Hg in the placebo group. - pubmed
Serum creatine kinase , aspartate aminotransferase and plasma progestogen concentrations were measured . - pubmed
Plasma PBI concentration , plasma and pituitary TSH , contents , and hypothalamic TRH content were measured by RIA in basal and stimulated conditions . - pubmed
Cross - reactivity of the antisera raised against isolated subunits of the kinase was assessed by immunodiffusion analysis and by measuring the cAMP binding and phosphotransferase activities of the subunits after immunoprecipitation . - pubmed
Calmodulin and Ca2 + in normal and transformed cells . - pubmed
Numerous lines of evidence implicate calcium and calmodulin ( CaM ) as regulators of cell growth and functional differentiation . - pubmed
This paper summarizes the evidence linking calcium and CaM to the regulation of cell growth and critically examines the evidence that increases in CaM levels occur in transformed versus normal cells . - pubmed
URF1 , encoding an Mr 13500 polypeptide at genome coordinate 17 , is predominantly conserved in nucleotide and amino acid sequence , but contains one half as many arginine amino acids as does URF1 of Ad2 . - pubmed
URF2 , encoding an Mr 13600 protein which lies within the i - leader region , is not well conserved in either nucleotide or amino acid sequence . - pubmed
CaP were produced in fibers superfused with a Na +-free ( substituted by tetraethylammonium chloride ) high Ca ++ ( 16.2 mM ) Tyrode 's solution ( pH 7.4 or pH 6.0 ) . - pubmed
It was possible to block low pH , CaP with verapamil alone , while high RMP , high pH , CaP could be totally blocked only with a combination of verapamil plus tetrodotoxin . - pubmed
As the blood glucose content ( indicating experimental diabetes gravity ) rises , the aldose reductase activity in the tissues increases , the content of sorbitol and fructose ascends . - pubmed
An increase of aldose reductase and a decrease in the sorbitol - dehydrogenase activity occur within the first two weeks after diabetes induction , which correlates with the maximal rate of sorbitol and fructose accumulation . - pubmed
These pressor responses could be significantly attenuated by pretreatment with the dopamine D2 receptor antagonist haloperidol , but not the D1 receptor antagonist SCH 23390 . - pubmed
Chemical stimulation of the VTA , by microinjection of 10 nmol of the substance P analogue DiMe - C7 , produced a sustained increase in blood pressure ( max 10 - 15 mmHg ) , which could be completely prevented by pretreatment with haloperidol . - pubmed
The Ca ( 2 +)-independent specific NOS activity is about 30 % of the Ca ( 2 +)-dependent microsomal activity . - pubmed
The microsomal Ca ( 2 +)-dependent NOS activity is inhibited by 50 % in the presence of 0.5 mM aminoguanidine ( AG ) , whereas the Ca ( 2 +)-independent activity does not respond to this concentration of AG , suggesting that it is not the inducible isoform of NOS . - pubmed
Our results indicate that ( I ) NOS activity is present from an early phase of placental development , ( II ) the NOS activity is of trophoblastic origin , since the primordial placenta is avascular and ( III ) NO - production by the primordial placenta can proceed in the absence of any Ca ( 2 +)-mobilizing agonist . - pubmed
Plasma concentrations of FSH , LH , progesterone , testosterone and oestradiol-17 beta were also determined by RIAs . - pubmed
The changes in plasma concentrations of FSH after injection of antisera to oestradiol-17 beta ( oestradiol - AS ) and inhibin ( inhibin - AS ) on the morning of day 2 ( day 1 = day of ovulation ) were also determined . - pubmed
Plasma concentrations of oestradiol-17 beta showed three increase phases and these changes differed from those of inhibin . - pubmed
During repair of crush injury , however , the level of antigen drops concomitant with the ingrowth of regenerating axons , suggesting axonal control of A-1 antigen expression . - pubmed
[ 18F ] Benperidol ( [ 18F ] BP ) , a positron - emitting analogue of the dopaminergic D2 antagonist benperidol , was evaluated as a radiopharmaceutical for use with positron emission tomography ( PET ) . - pubmed
[ 18F ] BP bound to D2 receptors in a reversible manner ; unlabeled eticlopride displaced D2 receptor - bound [ 18F ] BP in vivo . - pubmed
[ 18F ] BP has advantages over other tracers as a radiopharmaceutical for PET study of central D2 receptor activity , and can be applied for noninvasive evaluation of the interaction of unlabeled drugs with central D2 receptor sites . - pubmed
These results demonstrate that PF4 and TGF beta 1 inhibit MK development from CD34 + CB cells by different mechanisms and suggest that PF4 , unlike TGF beta 1 , exerts its inhibitory effect on cell growth in a reversible and S phase - specific manner by which it protects stem cells and MK progenitor cells from 5 - FU cytotoxicity . - pubmed
NMR characterization of structure , backbone dynamics , and glutathione binding of the human macrophage migration inhibitory factor ( MIF ) . - pubmed
Human macrophage migration inhibitory factor is a 114 amino acid protein that belongs to the family of immunologic cytokines . - pubmed
From the total of three cysteines present in the primary structure of MIF , none was found to form disulfide bridges . - pubmed
The capacity to bind glutathione was investigated by titration of a uniform 15N - labeled sample and led us to conclude that MIF has , at best , very low affinity for glutathione . - pubmed
OBJECTIVE : To investigate the role of platelet activating factor ( PAF ) in the hypoxic pulmonary hypertension . - pubmed
Discrimination of conformational states of mitochondrial cytochrome P-450scc by selective modification of several Lys residues . - pubmed
Selective serotonin reuptake inhibitors in affective disorders -- II . - pubmed
CB1 message expression in all three brain areas returned to vehicle control levels by day 21 of Delta9 - THC treatment , a time at which behavioral tolerance has been previously reported . - pubmed
Comparisons suggests that CB1 message may be regulated by different effector systems in each of the three areas during chronic Delta9 - THC exposure . - pubmed
However , methylation of the estrogen receptor - alpha gene ( ERalpha ) promoter blocks the expression of ERalpha , and thereby can antagonize the regulatory effect of estrogen on cell proliferation . - pubmed
Using a screening technique , RLGS - M , we show that alteration in methylation associated with the smooth muscle cell phenotypic switch does not seem to require heightened activity of the methyltransferase enzyme , and appears to be selective for the ERalpha and a limited pool of genes whose CpG island becomes either demethylated or de novo methylated . - pubmed
contains two malate dehydrogenase isoforms , a mitochondrial isoenzyme with a high specificity for oxaloacetate and a glycosomal isozyme that acts on a broad range of substrates ( Uttaro , A. D. , and Opperdoes , F.R. - pubmed
One isoform behaved as a real malate dehydrogenase with a high specificity for oxaloacetate , whereas the other showed no activity with oxaloacetate but was able to reduce other oxoacids , such as phenyl pyruvate , 2 - oxoisocaproate , 2 - oxovalerate , 2 - oxobutyrate , 2 - oxo-4 - methiolbutyrate , and pyruvate . - pubmed
SB202190 , a p38 - specific inhibitor , inhibits UVB - induced p38 activation and c - fos gene expression . - pubmed
Furthermore , SB202190 markedly inhibited UVB - induced AP-1 DNA binding as determined by mobility shift analyses . - pubmed
Alanine racemases are ubiquitous , almost uniquely prokaryotic enzymes catalyzing the racemization between l - and d - alanine . - pubmed
Water immersion stress prevents caerulein - induced pancreatic acinar cell nf - kappa b activation by attenuating caerulein - induced intracellular Ca2 + changes . - pubmed
NF - kappaB is a proinflammatory transcription factor activated during caerulein pancreatitis . - pubmed
Chelation of intracellular Ca ( 2 + ) by 1,2 - bis ( 2 - aminophenoxy ) ethane - N , N , N ' , N '- tetraacetate / acetoxymethyl ester has similar effects to water immersion in preventing caerulein but not TNF - alpha - induced NF - kappaB activation in pancreas . - pubmed
Our findings indicate that , in addition to Ca ( 2 +)-dependent mechanisms , Ca ( 2 +)-independent signaling events also may lead to NF - kappaB activation in pancreatic acinar cells . - pubmed
Water immersion stress prevents supramaximal caerulein - induced NF - kappaB activation in pancreas in vivo and in vitro by affecting intracellular Ca ( 2 + ) homeostasis . - pubmed
The resulting triacylglycerols were analyzed by gas chromatography ( GC ) with a 65 % phenyl methyl silicone capillary column and separated on the basis of both fatty acid composition and regiospecific position . - pubmed
The results showed that this method compares favorably to the existing methods for analysis of oleic , petroselinic , and cis - vaccenic fatty acids by GC with respect to peak separation and accuracy , and it also provides information on the regiospecific distribution of the fatty acids . - pubmed
Without the vitamin , membranes may have an abnormally high permeability and they may be subject to degradation by endogenous phospholipases in vivo , as well as being abnormally susceptible to damage in vitro by dialuric acid and hydrogen peroxide . - pubmed
In contrast , purified human Pol mu alone could only add three dGMPs as predicted from the sequence of the template . - pubmed
The production of the dGMP ladder at the primer terminus located opposite the AAF adduct reveals an unusual ability of Pol mu ( in conjunction with its cofactor ) to perform DNA synthesis from a slipped intermediate containing several unpaired bases . - pubmed
The differences in amino acid residues of HLA - B27 subtypes are minor , but may play role in pathogenesis of ankylosing spondylitis ( AS ) . - pubmed
Oral contraceptive use and increased plasma concentration of C - reactive protein . - pubmed
We examined , in young adult women , the association between current low dose oral contraceptive ( OC ) use and plasma levels of C - reactive protein ( CRP ) , an acute phase reactant predictive of cardiovascular disease risk . - pubmed
Mutations of charged amino acids in or near the transmembrane helices of the second membrane spanning domain differentially affect the substrate specificity and transport activity of the multidrug resistance protein MRP1 ( ABCC1 ) . - pubmed
Multidrug resistance protein 1 ( MRP1 ) belongs to the ATP - binding cassette superfamily of transport proteins . - pubmed
MRP1 consists of two membrane - spanning domains ( MSD2 and MSD3 ) each followed by a nucleotide binding domain plus a third NH2 - terminal MSD1 . - pubmed
MSD2 contains transmembrane ( TM ) helices 6 through 11 , and previously , we identified two charged residues in TM6 as having important but markedly different roles in MRP1 transport activity and substrate specificity by characterizing mutants containing nonconservative substitutions of Lys332 and Asp336 . - pubmed
We have now extended these studies and found that the same - charge TM6 mutant K332R , like the nonconservatively substituted Lys332 mutants , exhibits a selective decrease in leukotriene C4 ( LTC4 ) transport , associated with substantial changes in both Km and Vmax and LTC4 binding . - pubmed
However , unlike TM6 Asp336 , wild - type transport activity could be reestablished in these MRP1 mutants by conservative substitutions . - pubmed
Decreasing oxygen concentrations cause strong attenuation of PEDF release resulting in enhanced VEGF / PEDF ratios . - pubmed
However , it caused a GDP - inhibition of state 4 respiration and a GDP - induced re - polarization of the inner mitochondrial membrane in the presence of fatty acids , in agreement with its expected physiological behavior as an uncoupling protein ( UCP ) . - pubmed
Finally , the induction of uncoupling protein-3 also increased the sensitivity of mitochondria to open the permeability transition pore in response to calcium . - pubmed
Consumption of nitric oxide by endothelial cells : evidence for the involvement of a NAD ( P ) H - , flavin - and heme - dependent dioxygenase reaction . - pubmed
All experiments were performed in the presence of superoxide dismutase to minimize the peroxynitrite reaction . - pubmed
Both NO consumption and nitrate formation were largely inhibited by the heme poisons NaCN and phenylhydrazine as well as the flavoenzyme inhibitor diphenylene iodonium . - pubmed
We found that : ( 1 ) the ratio between the enrichment of alanine C3 and that of glutamate C4 , gamma - aminobutyric acid ( GABA ) C2 , glutamine C4 , or aspartate C3 decreased from pentobarbital to alphachloralose and morphine whatever the labeled precursor , indicating a link between metabolic and cerebral activity ; ( 2 ) under glucose + [ 3 -( 13 ) C ] lactate infusion , alanine C3 and acetyl - CoA C2 enrichments were higher than that of lactate C3 , revealing the occurrence of an isotopic dilution of the brain exogenous lactate ( arising from the blood ) by lactate from the brain ( endogenous lactate ) , and that the latter was synthesized from glycolysis in a compartment other than neurons ; and ( 3 ) the contribution of labeled glucose and lactate to acetyl - CoA and amino acid enrichment indicated that the involvement of blood glucose relative to that of blood lactate to brain metabolism was correlated with cerebral activity . - pubmed
Acetyl - coenzyme A carboxylases ( ACCs ) have crucial roles in fatty acid metabolism . - pubmed
Unexpectedly , our structures suggest that soraphen may bind in the BC dimer interface and inhibit the BC activity by disrupting the oligomerization of this domain . - pubmed
Cyclosporine A , alpha - lnterferon and interleukin-2 following chemotherapy with BCNU , DTIC , cisplatin , and tamoxifen : a phase II study in advanced melanoma . - pubmed
Preclinical data suggest that one method of inducing autoimmunity to tumor is the administration and subsequent withdrawal of cyclosporine A following chemotherapy and that this effect may be enhanced with interferon and interleukin-2 . - pubmed
Cyclosporine A at 3 mg / kg / day in two divided doses was given on days 4 - 21 , alpha - interferon 1 million units / m2 subcutaneously every other day on days 4 - 21 and interleukin-2 1 million units / m2 BID subcutaneously days 21 - 28 were also given . - pubmed
Enzyme - catalyzed regio- and enantioselective ketone reductions . - pubmed
The resulting propargylic alcohols can be obtained in either enantiomeric form , since ( R)- and ( S)-specific oxidoreductases can be applied . - pubmed
When used in treatment of postoperative nausea and vomiting , a situation where prolongation of the QTc interval seems to occur , the safety of 5 - hydroxytryptamine type 3 antagonists may not be superior to that of low - dose droperidol . - pubmed
Is relaxin a calcium transporter / buffer ? - pubmed
Relaxin inhibits smooth muscle contractions by binding Ca2 + and making it unavailable to the contractile elements . - pubmed
In the presence of relaxin , only 4 % of the 45Ca was on the filter with the SR as compared to that measured on the filter with SR alone or in the presence of proteins of similar isoelectric point ( cytochrome C ) and size ( insulin ) . - pubmed
A peak of 45Ca ( & gt;600 K cpm ) was found with the eluted relaxin . - pubmed
No peak of 45Ca was associated with cytochrome C or insulin under the same conditions . - pubmed
The eluted fractions containing 45Ca and relaxin were combined . - pubmed
Thus , 45Ca was not tightly bound to relaxin or the antibodies knocked the 45Ca off . - pubmed
Is relaxin a calcium transporter or buffer ? - pubmed
AIM : Adenosine has many actions potentially useful as adjunct to a cardioplegia . - pubmed
RESULTS : No cross - reactivity of the antibody was observed with other aminoglycosides based on competitive direct ELISA methods , indicating that the antibody is highly specific for neomycin . - pubmed
Through this method , the detection limits were estimated to be about 10 ng / ml of neomycin in PBS , plasma , and milk . - pubmed
It hydrolyzed the synthetic substrates containing arginine residue in the P1 position but did not hydrolyze synthetic substrates containing other amino acid residues , including lysine residue in the P1 position . - pubmed
Among the synthetic substrates tested , a substrate of plasminogen activator ( Pyr - Gly - Arg - MCA ) was hydrolyzed most effectively with the enzyme ( Km = 5.5 x 10(-6 ) M ) , and fibrin gel was solubilized via activation of intrinsic plasminogen to plasmin with the enzyme . - pubmed
The serum LDL level , LDL / HDL ratio , ApoB and MDA in the Huajiao volatile oil group were significantly reduced , compared with those in model group . - pubmed
Novel genes for nitrite reductase and Amo - related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling . - pubmed
The insert encoded a homologue of a copper - containing nitrite reductase with an unusual C - terminus that encoded a potential amicyanin - like electron transfer domain as well as two proteins related to subunits of ammonia monooxygenases or particulate methane monooxygenases ( AmoAB / PmoAB ) respectively . - pubmed
Expression of nirK and the amoA - like gene was shown by reverse transcription polymerase chain reaction ( PCR ) analyses in soil samples , the latter being found at higher levels when the soil was incubated with ammonia ( measured by quantitative PCR ) . - pubmed
The molecular design made use of a genetically engineered chaperonin GroEL bearing , at both entrance parts of its cylindrical cavity , cysteine residues , which were functionalized by an azobenzene derivative to construct photoresponsive mechanical gates ( azo - GroEL ) . - pubmed
However , when hosting azo - GroEL detected ATP ( input stimulus 1 ) and UV light ( input stimulus 2 ) at the same time , it quickly released GFP(denat ) to allow its refolding . - pubmed
Activities of ribulose bisphosphate carboxylase and phosphoenolpyruvate carboxylase ( expressed in micromoles CO ( 2 ) per milligram protein per hour ) averaged 1.95 and 8.87 for the flower buds , and 28.5 and 3.6 for the leaves , respectively . - pubmed
Protein - bound ribulose bisphosphate correlates with deactivation of ribulose bisphosphate carboxylase in leaves . - pubmed
Previous reports indicate that ribulose 1,5 - bisphosphate ( RuBP ) binds very tightly to inactive ribulose bisphosphate carboxylase ( rubisco ) in vitro . - pubmed
Therefore , we decided to investigate whether there was evidence for tight binding of RuBP associated with deactivation of rubisco in vivo . - pubmed
Bound RuBP was significantly correlated with the estimated number of inactive rubisco sites , with a ratio of about 1:1 . - pubmed
After a step increase in irradiance , rubisco activation increased and total RuBP increased transiently , but steady levels of both occurred by 10 minutes . - pubmed
The amount of bound RuBP decreased with a similar time course to the estimated decrease in inactive rubisco sites . - pubmed
Bound RuBP increased gradually but did so more slowly than the estimated increase in inactive rubisco sites . - pubmed
The identification of the twin - pore channel , TASK , in pulmonary artery smooth muscle , along with reports that it is inhibited by hypoxia , raises the possibility that a member of this family of channels acts as an O2 sensor in pulmonary artery . - pubmed
Increasing evidence points to the importance of achieving low blood glucose variability and also a low hemoglobin A1c ( HbA1c ) to prevent diabetic late complications . - pubmed
Our data demonstrate that the nuclear interaction of Smac / DIABLO with Survivin is an important step for suppressing the anti - apoptotic function of Survivin in Doc - induced apoptosis . - pubmed
This suggests that the balance between cellular Smac / DIABLO and Survivin levels could be critical for cellular destiny in taxane - treated cancer cells . - pubmed
Effects of AM281 , a cannabinoid antagonist , on circulatory deterioration and cytokine production in an endotoxin shock model : comparison with norepinephrine . - pubmed
Both AM281 and NE inhibited endotoxin - induced increases in cytokine production , with significant differences observed among the three groups at 1 and 3 h after treatment . - pubmed
CONCLUSIONS : Although NE and AM281 both prevented endotoxin - induced deterioration of systemic hemodynamics , AM281 yielded better preservation of mesenteric blood flow and attenuation of cytokine production than NE . - pubmed
PURPOSE : RPE65 , a membrane - associated protein predominantly expressed in the retinal pigment epithelium , is the isomerohydrolase in the retinoid visual cycle . - pubmed
Three cysteine ( Cys ) residues , Cys231 , Cys329 , and Cys330 , in RPE65 have been shown to be palmitoylated and have been suggested to be responsible for its membrane association . - pubmed
The purpose of this study was to define the role of these Cys residues in palmitoylation , membrane association , and isomerohydrolase activity of RPE65 . - pubmed
METHODS : The three Cys residues in RPE65 were replaced by Alanine ( Ala ) with site - directed mutagenesis . - pubmed
RESULTS : Mutation of any single residue of these three Cys significantly reduced protein levels of RPE65 . - pubmed
Similar to wild - type RPE65 , however , all three single Cys mutants were predominantly present in the membrane fraction . - pubmed
Mutations of any one or two of these Cys substantially weakened the isomerohydrolase activity of RPE65 , whereas mutations of all three Cys ( triple mutant ) completely abolished the enzymatic activity . - pubmed
The structural distortions induced by the Cys mutations may be responsible for the mislocalization and decreased isomerohydrolase activities of RPE65 . - pubmed
WNK4 , as thiazides , shuts off NaCl reabsorption to stimulate Na / K exchange . - pubmed
BACKGROUND : Prostaglandins modulate cytokine release though increases in cAMP , regulating interleukin-6 and interleukin-10 . - pubmed
Ketoprofen inhibits cyclo - oxygenase activity and hence prostaglandin production . - pubmed
AIM : We hypothesized that ketoprofen would affect release of IL-6 and IL-10 and modulate the immune response . - pubmed
At the end of the experimental period , animals were sacrificed and the assay of the activity of alkaline phosphatase , myeloperoxidase , and antioxidants and the estimation of lipid peroxides were performed . - pubmed
burgdorferi genome for a canonical integrin binding motif , the RGD ( Arg - Gly - Asp ) tripeptide , revealed several candidate ligands for integrins . - pubmed
The transformation to amino derivatives decreased at high concentrations of TNT , indicative of inhibition of microbial TNT transformation . - pubmed
However , methylprednisolone and Tac promoted increased expression levels of Bcl-2 in all groups . - pubmed
This communication is mediated by cytokines , growth factors and prostaglandins and dissecting the role of these signaling molecules might be the key to positively manipulate muscle regeneration in the future . - pubmed
In addition , Rpd3p interacted directly with chromatin at boundary regions to deacetylate histone H4 at lysine 5 and at lysine 12 . - pubmed
Either the mutation of histone H4 at lysine 5 or a decrease in the histone acetyltransferase ( HAT ) activity of Esa1p abrogated the silencing phenotype associated with rpd3 mutation , suggesting a novel role for the H4 amino terminus in Rpd3p - mediated heterochromatin boundary regulation . - pubmed
A 6 - mer photocontrolled oligonucleotide as an effective telomerase inhibitor . - pubmed
The small , photocontrolled oligonucleotide shows effective telomerase inhibition , both in vitro and in vivo , only when light irradiated . - pubmed
Mechanism of glycoside hydrolysis : A comparative QM / MM molecular dynamics analysis for wild type and Y69F mutant retaining xylanases . - pubmed
Two - dimensional potential - of - mean - force calculations based upon molecular dynamics with the AM1 / OPLS method for wild - type BCX with a p - nitrophenyl xylobioside substrate in water clearly indicates a stepwise mechanism for glycosylation : the rate - determining step is nucleophilic substitution by Glu78 to form the covalently bonded enzyme - substrate intermediate without protonation of the leaving group by Glu172 . - pubmed
The intracellular calcium oscillations in response to extracellular Ca ( 2 + ) were of sinusoidal pattern and mediated by the phospholipase C / diacylglycerol / inositol 1,4,5 - trisphosphate pathway as revealed by a biosensor that detects the accumulation of diacylglycerol in the plasma membrane . - pubmed
The intracellular calcium oscillations in response to aromatic amino acids were of transient type , that is , Ca ( 2 + ) spikes that returned to baseline levels , and required an intact actin cytoskeleton , a functional Rho , Filamin A and the ion channel TRPC1 . - pubmed
The pretreatment of cells with forsythoside A , adding forsythoside A post infection of cells , and treatment of virus with forsythoside A were analysed . - pubmed
Additional experiment joining DiI and synaptophysin ( a pre - synaptic protein ) labeling suggested synaptic sites on dendritic shafts and spines . - pubmed
Eph tyrosine kinase receptors and their ephrin ligands are expressed in most adult stem cell niches and in many types of tumors . - pubmed
Optical resolution of n - butyl D - and L - lactates using immobilized lipase catalyst . - pubmed
n - Butyl D - and L - lactates ( BuDLa and BuLLa ) were incubated with immobilized lipase . - pubmed
The enzyme was shown to be specific for the UDP - Gal donor substrate and required pyrophosphate in the acceptor substrate . - pubmed
Anti - thrombotic effects of selective estrogen receptor modulator tamoxifen . - pubmed
Tamoxifen is a known anti - cancer drug and established estrogen receptor modulator . - pubmed
The mass flux of PFAAs from stormwater runoff in the Twin Cities ( Minneapolis and St. Paul , MN ) metropolitan area is estimated to be about 7.86 kg / year . - pubmed
falciparum infections acquired in Honduras carried pfcrt , pfmdr1 , pfdhps and pfdhfr alleles associated with chloroquine , amodiaquine and sulphadoxine - pyrimethamine sensitivity only . - pubmed
BACKGROUND : Electrophysiological studies of L - type Ca2 + channels in isolated vascular smooth muscle cells revealed that depolarization of these cells evoked a transient and a time - independent Ca2 + current . - pubmed
The Ca2 +/Mn2 + ion - pump PMR1 links elevation of cytosolic Ca ( 2 + ) levels to alpha - synuclein toxicity in Parkinson ' s disease models . - pubmed
The protein alpha - synuclein ( alphaSyn ) , the principle toxic effector in PD , has been shown to interfere with neuronal Ca ( 2 + ) fluxes , arguing for an involvement of deregulated Ca ( 2 + ) homeostasis in this neuronal demise . - pubmed
Here , we identify the Golgi - resident Ca ( 2 +)/ Mn ( 2 + ) ATPase PMR1 ( plasma membrane - related Ca ( 2 +)- ATPase 1 ) as a phylogenetically conserved mediator of alphaSyn - driven changes in Ca ( 2 + ) homeostasis and cytotoxicity . - pubmed
In conclusion , the alphaSyn - driven rise of cytosolic Ca ( 2 + ) levels is pivotal for its cytotoxicity and requires PMR1 . - pubmed
Insulin sensitivity was assessed with the oral glucose insulin sensitivity ( OGIS ) index . - pubmed
Insulin secretion was estimated using three model - based parameters of insulin secretion ( beta - cell glucose sensitivity , the potentiation factor ratio , and beta - cell rate sensitivity ) . - pubmed
After complete digestion of the nucleic acids with the RNases and DNases , the newly added DNA - modified with the dNTP analogs , bonds firmly to the NRNs . - pubmed
Fibrin clot extension on zirconia surface for dental implants : a quantitative in vitro study . - pubmed
The glucagon - stimulated C - peptide significantly increased in treated cases compared to controls ( p = 0.036 ) . - pubmed
This result suggests that truncated Myo52p lacking cargo , or single molecules of wild - type Myo52p with small cargoes , can undergo processive movement along actin - Cdc8p cables in vivo . - pubmed
BACKGROUND : Artemisinin - based combination therapy ( ACT ) is widely recommended as first - line therapy for uncomplicated Plasmodium falciparum malaria worldwide . - pubmed
Central to the formation of tertiary structure in membrane protein folding is the presence of amino acid sequence motifs ( such as " small - XXX - small " segments ) in the TM segments that promote interaction - compatible surfaces through which the TM alpha - helices interact . - pubmed
In this context , we used proteolipid protein ( PLP ) , the major protein from central nervous system myelin for which mutant - dependent non - native oligomerization has been implicated in neurological disorders , to explore the specific effects of TM boundary residues ( the membrane entry and exit points ) , keying on the secondary structure and self - association of peptides corresponding to the PLP TM2 alpha - helix ( wild - type sequence 66AFQYVIYGTASFFFLYGALLLAEGF90 ) . - pubmed
The increase in proteinogenic amino acid concentrations together with the increase in glutamate dehydrogenase activity suggests a requirement for nitrogen metabolism . - pubmed
After 7 days , alizarin red staining , alkaline phosphatase ( ALP ) assays , and qRT - PCR were used to assess the mineralization , expression of ALP , and gene expression ( collagen type 1 and osteocalcin ) , respectively . - pubmed
Alizarin red and alkaline phosphatase staining showed mineralization in all three groups . - pubmed
At termination , CAN was assessed in the renal tissue by light and electron microscopy , TG2 and epsilon(gamma - glutamyl )- lysine by immunofluorescence , and the urinary epsilon(gamma - glutamyl )- lysine by high performance liquid chromatography . - pubmed
Extracellular TG2 ( glomerulus : 64.55 + - 17.61 versus 2.11 + - 0.17 , P & lt ; 0.001 ; interstitium : 13.72 + - 1.62 versus 3.19 + - 0.44 , P & lt ; 0.001 ) , epsilon(gamma - glutamyl )- lysine ( glomerulus : 21.74 + - 2.71 versus 1.98 + - 0.37 , P & lt ; 0.01 ; interstitium : 37.96 + - 17.06 versus 0.42 + - 0.11 , P & lt ; 0.05 ) , TG2 enzyme activity ( 1.09 + - 0.13 versus 0.41 + - 0.03 nmol / h / mg protein , P & lt ; 0.05 ) , TG2 mRNA ( 20 - fold rise ) , and urinary epsilon(gamma - glutamyl )- lysine ( 534.2 + - 198.4 nmol/24 h versus 57.2 + - 4.1 nmol/24 h , P & lt ; 0.05 ) levels were significantly elevated in the allografts and showed a positive linear correlation with tubulointerstitial fibrosis . - pubmed
CONCLUSION : CAN was associated with upregulation of renal TG2 pathway , which has a potential for pharmacological intervention . - pubmed
HIF-1alpha signaling activation by post - ischemia treatment with astragaloside IV attenuates myocardial ischemia - reperfusion injury . - pubmed
In this study , we evaluated the effect of astragaloside IV ( Ast IV ) post - ischemia treatment on myocardial ischemia - reperfusion ( IR ) injury ( IRI ) . - pubmed
Additionally , in cardiomyocytes , 2 - MeOE2 and HIF-1alpha siRNA treatment each not only abolished the anti - apoptotic effect of post - ischemia treatment with Ast IV but also reversed the upregulation of HIF-1alpha and iNOS expression . - pubmed
2 - MeOE2 reversed these effects of Ast IV on IR - injured hearts . - pubmed
A NELL-1 isoform with the N - terminal 240 amino acid ( aa ) residues truncated was identified . - pubmed
Parthenolide induces autophagy via the depletion of 4E - BP1 . - pubmed
Inhibition of autophagy by knocking out autophagy associated gene atg5 blocked PTL - induced apoptosis . - pubmed
Surprisingly , PTL decreased the level of translation initiation factor eIF4E binding protein 1 ( 4E - BP1 ) in correlation with autophagy . - pubmed
Ectopic expression or shRNA knockdown of 4E - BP1 further verified the effect of 4E - BP1 on PTL - induced autophagy . - pubmed
Meanwhile , PTL elevated the cellular reactive oxygen species ( ROS ) which located upstream of the depletion of 4E - BP1 , and contributed to the consequent autophagy . - pubmed
This study revealed 4E - BP1 as a trigger for PTL - induced autophagy and may lead to therapeutic strategy to enhance the efficacy of anticancer drugs . - pubmed
Using data from a randomized controlled trial evaluating computerized CBT ( CBT4CBT ) , we evaluated treatment response by COMT genotype , with the a priori hypothesis that Val carriers would have improved response to computerized delivery of CBT . - pubmed
There was a significant treatment condition by genotype interactions for rates of participants attaining 21 or more days of continuous abstinence as well as self - reported percent days of abstinence , suggesting less cocaine use among Val carriers when assigned to CBT compared to standard treatment . - pubmed
Exploration of possible mechanisms using measures of attentional biased also pointed to greater change over time in these measures among the Val carriers assigned to CBT . - pubmed
CONCLUSION : These are the first data from a randomized controlled trial indicating significant interactions of COMT polymorphism and behavioral therapy condition on treatment outcome , where Val carriers appeared to respond particularly well to computerized CBT . - pubmed
Our aim was to study the effect of the non - selective MMP and TNF - alpha converting enzyme ( TACE ) inhibitor GM6001 ( 30 mg / kg ) on anastomosis repair in obstructed left colon . - pubmed
In vitro , GM6001 also delayed ( p=0.026 ) epithelialization of denuded intestinal epithelium grown on type I collagen . - pubmed
CONCLUSIONS : Non - selective MMP / TACE inhibition with GM6001 increased the anastomotic complications following colon obstruction . - pubmed
The effect of SPRY4 - IT1 on proliferation was evaluated by MTT and colony formation assays . - pubmed
Olive Oil - derived Oleocanthal as Potent Inhibitor of Mammalian Target of Rapamycin : Biological Evaluation and Molecular Modeling Studies . - pubmed
The established anticancer and neuroprotective properties of oleocanthal combined with the reported role of mammalian target of rapamycin ( mTOR ) in cancer and Alzheimer ' s disease development encouraged us to examine the possibility that oleocanthal inhibits mTOR . - pubmed
To validate this hypothesis , we docked oleocanthal into the adenosine triphosphate binding pocket of a close mTOR protein homologue , namely , PI3K - gamma . - pubmed
Apparently , oleocanthal shared nine out of ten critical binding interactions with a potent dual PIK3 - gamma / mTOR natural inhibitor . - pubmed
Subsequent experimental validation indicated that oleocanthal indeed inhibited the enzymatic activity of mTOR with an IC50 value of 708 nM. - pubmed
These results strongly indicate that mTOR inhibition is at least one of the factors of the reported anticancer and neuroprotective properties of oleocanthal . - pubmed
Predictors of PDB included older age , lower baseline hemoglobin , lower platelet reactivity on clopidogrel , and use of chronic oral anticoagulation therapy . - pubmed
The receptor for gonadotropin - releasing hormone ( GnRH ) belongs to the G protein - coupled receptors ( GPCRs ) , and its stimulation activates extracellular signal - regulated protein kinase ( ERK ) . - pubmed
We found also that GnRH induced the cleavage of ErbB4 . - pubmed
In the present study , we examined signal transduction for the activation of ERK and the cleavage of ErbB4 after GnRH treatment . - pubmed
Both ERK activation and ErbB4 cleavage were completely inhibited by YM-254890 , an inhibitor of Gq / 11 proteins . - pubmed
Experiments with two types of PKC inhibitors , Go 6976 and bisindolylmaleimide I , indicated that novel PKC isoforms but not conventional PKC isoforms were involved in ERK activation and ErbB4 cleavage . - pubmed
Our experiments indicated that the novel PKC isoforms activated protein kinase D ( PKD ) after GnRH treatment . - pubmed
After Cu exposure , SOD ( superoxide dismutase ) , GPx ( glutathione peroxidase ) , GR ( glutathione reductase ) and GST ( glutathione S - transferase ) activities increased and CAT ( catalase ) activity decreased in the liver at water at pH 4.5 and 8.0 . - pubmed
SOD and CAT activities increased in the white muscle after Cu exposure at pH 8.0 and GPx , GR and GST activities decreased after Cu exposure at pH 4.5 and 8.0 . - pubmed
The PAM stabilizes favorable agonist interactions , while Na (+ ) tends to disrupt agonist binding . - pubmed
We identified two residues involved in allosteric signal transmission : Trp 7.35 at the top and Tyr 7.53 at the bottom of TM VII . - pubmed
Gallic acid at 30 mg kg(-1 ) decreased the levels of protein expression of caspase-3 and iNOS induced by I / R injury . - pubmed
This effect of gallic acid was mainly mediated by reducing protein expression of iNOS and caspase-3 . - pubmed
Proto - oncogene tyrosine - protein kinase ( Src ) was identified as its membrane target and the dissociation constant ( Kd ) between Src and isorhamnetin was 3.81 muM. - pubmed
Furthermore , anti - leukemia effects of isorhamnetin were mediated by Src through inducing G2 / M cell cycle arrest . - pubmed
In this framework , by first processing the time - varying TRs , a finite number of uncertain but time - constant TR matrices are obtained . - pubmed
AIM : The changes of the hypothalamic pituitary adrenal ( HPA ) axis response to a long distance transportation results in increase of adrenocorticotropic hormone ( ACTH ) and cortisol levels . - pubmed
RESULTS : One - way RM - ANOVA showed significant effects of transport on ACTH changes in Groups B and C and on cortisol changes in both Groups A and B. - pubmed
In this study , we hypothesized that serotonin ( 5 - HT ) , acting in the retrotrapezoid nucleus / parafacial respiratory group ( RTN / pFRG ) , is able to generate active expiration . - pubmed
In the H - H sigma - bond activation of a dihydrogen molecule by a nickel ( 0 )- borane complex , the empty p orbital of the borane moiety interacts with the H - H sigma bonding MO to form charge transfer ( CT ) from the dihydrogen molecule to the borane moiety to accelerate the reaction . - pubmed
In the B - F sigma - bond activation of BF3 by a platinum ( 0 )- bisphosphine complex , the second BF3 molecule interacts with the F atom that is dissociating from the B atom to stabilize the transition state and product by the CT from the F atom to the second BF3 . - pubmed
The n - alkanes of all samples had a bimodal distribution with the 1st peak at n - alkane with 17 carbons ( C17 ) and the 2nd predominant peak at C29 . - pubmed
SOM received a mixed contribution of plankton ( I ) , low - latitude terrestrial high - grade plants ( II ) and microbial material ( III ) as indicated by isotopic compositions of long - chain n - alkane . - pubmed
Tafazzin is involved in the biosynthesis of the unique phospholipid cardiolipin ( CL ) , which is almost exclusively found in mitochondrial membranes . - pubmed
Extended dialysis with low calcium dialysate concentration and citrate anticoagulation , and administration of teriparatide led to a further increase in bone - specific alkaline phosphatase activity and most importantly , resulted in an activated bone turnover as confirmed by a second bone biopsy 11 weeks later . - pubmed
PURPOSE : Urine prostaglandin E2 ( PGE2 ) levels have shown to be a risk factor of breast cancer , and the use of nonsteroidal anti - inflammatory drugs ( NSAIDs ) is known to be beneficial in preventing breast cancer risk and/or recurrence with or without aromatase inhibitors . - pubmed
We hypothesized that the use of an aromatase inhibitor triggers the activation of the inflammatory pathway via release of PGE2 . - pubmed
Adults with higher vitamin C levels exhibited lower weight , BMI and waist circumference , and better measures of metabolic health , including HbA1c , insulin and triglycerides , all risk factors for type 2 diabetes . - pubmed
However , the EPS concentration and dehydrogenase activity decreased constantly with increasing AMP dosage . - pubmed
Treatment with corticosteroids and cyclosporine ( CsA ) resulted in a decrease of the methylation level of GATA3 and TGF - beta in inactive BD patients . - pubmed
Compared with the control and saline groups , IOF of CrPyr increased the plasma creatine concentration on the day of hatch , and the plasma pyruvate concentration on the day of hatch and 3 day post - hatch ( p & lt ; .05 ) . - pubmed
Enhanced hemoglobin - oxygen unloading in migratory salmonids . - pubmed
Recent findings indicate that some teleost fishes may be able to greatly enhance hemoglobin - oxygen ( Hb - O2 ) unloading at the tissues under conditions that result in catecholamine release . - pubmed
Together , these results indicate that a system to enhance Hb - O2 unloading may be common among salmonids and may be operational even under routine conditions . - pubmed
The EGFP expression was attenuated by treatment with an inhibitor for ERK , TGF - beta or reactive oxygen species ( ROS ) signaling . - pubmed
Resveratrol protects early brain injury after subarachnoid hemorrhage by activating autophagy and inhibiting apoptosis mediated by the Akt / mTOR pathway . - pubmed
In the present study , we examined the effect of resveratrol on EBI and their potential relationship with the Akt / mTOR pathway , autophagy , and apoptosis . - pubmed
Immunohistochemistry analysis of beclin-1 , LC3 - B treated with resveratrol alone or in combination with 3 - methyladenine ( autophagy inhibitor ) suggested that resveratrol induced the autophagy process and the inhibitor blocked the occurrence of autophagy , and also increased the number of terminal deoxynucleotidyl transferase - mediated digoxigenin - DUTP - biotin nick - end labeling ( + ) cells . - pubmed
Taken together , these findings indicate that resveratrol exerts neuroprotective effects on EBI after SAH by regulating autophagy and apoptosis mediated by the Akt / mTOR pathway . - pubmed
To test this hypothesis , we evaluated DNA damage along with comprehensive quantification of RNA transcripts ( RNA - seq ) , identification of accessible chromatin ( ATAC - seq ) , and characterization of regions with histone modifications associated with active transcription ( ChIP - seq for acetylation at histone 3 lysine 27 , H3K27ac ) . - pubmed
At follow - up , no significant differences in cortical or medullary R2 * values between AVT and AnonVT were observed , and the GFR decline was similar in the two groups ( 3.0 vs. 3.3 ml / min per 1.73 m ) . - pubmed
Ectopic expression of OsWRKY42 results in reduced expression of cell wall damage and salt stress induced jasmonic acid biosynthesis and response genes . - pubmed
CONCLUSION : The results presented here suggest that OsWRKY42 regulates plant responses to either cell wall damage or salinity stress by acting as a negative regulator of jasmonic acid mediated responses . - pubmed
This work provides a rigorous procedure , within the framework of the Reaction Class Transition State Theory ( RC - TST ) and the Structure - Activity Relationship ( SAR ) , for predicting reliable thermal rate constants on - the - fly for hydrogen abstraction reactions by methyl / ethyl radicals from Polycyclic Aromatic Hydrocarbons ( PAHs ) in a temperature range of 300 - 3000 K. All necessary RC - TST parameters were derived from ab initio calculations for a representative set of 36 reactions on which different error analyses and comparisons with available literature data were carried out . - pubmed
Therefore , the constructed RC - TST procedure can be confidently used to obtain reliable rate constants on the fly in an attempt to effectively construct detailed kinetic mechanisms for PAH - related fuels . - pubmed
According to the sequential extraction procedure ( SEP ) the loosely bounds Cr ( 90.28 % ) was converted to the relatively strong bound ( Fe - Mn oxides fraction , 92.09 % ) revealed the significant decrease in risk of release and availability of chromium after immobilization procedure . - pubmed
The microstructure and microhardness evolution of a Ti-15(wt.%)Mo / TiB metal - matrix composite ( MMC ) during high - pressure torsion ( HPT ) at 400 C was studied . - pubmed
An increase in dislocation density , a considerable decrease in a grain size in the bcc Ti matrix , and breaking / rearrangement of the TiB whiskers were observed during HPT . - pubmed
Its versatility lies in the capacity to form eight different inter - ubiquitin linkages through the seven lysine residues of ubiquitin and through its N - terminal methionine ( M1 ) . - pubmed
Here , we show that the histone deacetylase inhibitor , trichostatin A ( TSA ) , suppresses HER2 - overexpressing breast cancer via upregulation of miR-146a and the resultant repression of its oncogenic targets , interleukin-1 receptor - associated kinase 1 and the chemokine receptor CXCR4 . - pubmed
Consequently , TSA synergizes with lapatinib , a tyrosine kinase inhibitor of HER2 , to suppress breast cancer in vitro and in rodent models . - pubmed
Targeting lactate dehydrogenase - A promotes docetaxel - induced cytotoxicity predominantly in castration - resistant prostate cancer cells . - pubmed
LDH controls the conversion of pyruvate to lactate , with LDH - A , one of the predominant isoforms of LDH , controlling this metabolic process . - pubmed
Sodium oxamate ( SO ) was used as a specific LDH - A inhibitor . - pubmed
Cell cycle progression and apoptotic inducibility were evaluated by flow cytometry using propidium iodide and Annexin V staining . - pubmed
LDH expression was strongly associated with DOC sensitivity in PC cells . - pubmed
The results strongly indicated that LDH - A serves an important role in DOC resistance in advanced PC cells and inhibition of LDH - A expression promotes susceptibility to DOC , particularly in CRPC cells . - pubmed
Differences of cyclin - dependent kinase 4 / 6 inhibitor , palbociclib and abemaciclib , in breast cancer . - pubmed
Both palbociclib and abemaciclib are , oral , highly selective inhibitors of cyclin - dependent kinase 4 and 6 , which are proteins involved in cell differentiation and growth . - pubmed
In pivotal phase III trials ( PALOMA and MONARCH ) , they demonstrated a significant improvement in median progression - free survival in combination with a nonsteroidal aromatase inhibitor in the first - line , and with a fulvestrant in the second - line in hormone receptor - positive and HER2 - negative metastatic breast cancer , respectively . - pubmed
Both palbociclib and abemaciclib were approved , however , ribociclib , the third cyclin - dependent kinase 4 / 6 inhibitor , has not been approved in Japan . - pubmed
In contrast , additional benefits from abemaciclib in combination with nonsteroidal aromatase inhibitor or fulvestrant seem to have a relationship with visceral disease , liver metastasis , primary resistant to endocrine therapy , and short treatment - free interval . - pubmed
Filtrate analysis after NaOH , NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars ( SRS ) in range of ~0.17 - 0.44 % , ~0.38 - 0.75 % and ~2.9 - 8.4 % respectively . - pubmed
Release of soluble components other than SRS , majorly reported to be soluble lignins , were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments . - pubmed
Application of commercial cellulase increased raw SCB saccharification from 1.93 % to 38.84 % , 25.56 % and 9.61 % after NaOH , H2SO4 and NH4OH pretreatments . - pubmed
The Tx100 - only protocol retained 1.5 times more collagen and 2.5 times more sGAG than the livers decellularized with Tx100 + SDS . - pubmed
X - ray diffraction studies confirmed that complexes [ 1]-[4 ] feature a theophyllinato ligand coordinated to the metal center in the rarely observed , chelating fashion via the N7 and O1 atoms . - pubmed
We aimed to evaluate the additive and prognostic value of pre - treatment 2 -[ 18F ] fluoro-2 - deoxy - d - glucose positron emission tomography / computed tomography ( 2 -[ 18F ] FDG PET / CT ) in the staging and management of ACa compared to magnetic resonance imaging ( MRI ) . - pubmed
Pre - treatment 2 -[ 18F ] FDG PET / CT studies and MRI reports were compared considering the primary tumor , pelvic lymph nodes , and metastatic lesions . - pubmed
The impact of 2 -[ 18F ] FDG PET / CT in the management and its prognostic value , using maximum standardized uptake value ( SUVmax ) , were assessed . - pubmed
Insulin clearance has recently been highlighted as a fundamental aspect of glucose metabolism , as it has been hypothesized that its impairment could be related to an increased risk of developing type 2 diabetes . - pubmed
The most common PCP - related NACDG allergens for both sexes were methylisothiazolinone(MI ) ( MPCP:28.8 % , FPCP:21.5 % ) , fragrance mix I ( 22.3 % , 20.1 % ) , balsam of Peru ( 18.5 % , 14.1 % ) , quaternium-15 ( 16.1 % , 12.3 % ) , and paraphenylenediamine ( 11.5 % , 11.1 % ) . - pubmed
The bd quinol oxidase with a higher binding affinity for O2 than the aa 3 oxidase is known to play an important role in aerobic respiration under oxygen - limiting conditions . - pubmed
Above - 31 cm depth in the core , TN , TP , TOC , C / N , and delta13C increased significantly , indicating that eutrophication has occurred since the 1980s . - pubmed
The binding site for rabbit skeletal alpha - tropomyosin on troponin - T has been localized to the cyanogen bromide fragment , CB2 ( residues 71 - 151 ) , using affinity chromatography . - pubmed
It is concluded that the helical region of CB2 , and perhaps the carboxyl side chains of aspartic and glutamic acids , may be involved in binding over a limited surface area of the double - stranded coiled coil of alpha - tropomyosin . - pubmed
Mu - chain protein GLI is a pentameric molecule with an amino - terminal deletion comprising 130 residues . - pubmed
The half - cysteine residue ( position 140 ) which forms the H - L disulfide bridge in normal IgM is present . - pubmed
It is concluded that after dbcAMP stimulation , Sertoli cells produce 29 - kDa inhibin and the alpha - subunit precursor proteins pro - alpha C and alpha N. - pubmed
The production of the alpha - subunit precursor in addition to inhibin provides an explanation for the decrease in the inhibin B : I ratio following dbcAMP stimulation of Sertoli cells in culture . - pubmed
The seed proteins are rich in most of the essential amino acids , and they are deficient only in cystine and threonine , when compared to the WHO / FAO requirement pattern . - pubmed
The antinutritional factors ( total free phenols , tannins , trypsin inhibitor activity and haemagglutinating activity ) were also investigated . - pubmed
In pancreatic islets the bulk of phosphoinositide - specific phospholipase C ( PI - PLC ) activity was cytosolic . - pubmed
The soluble enzyme was activated by submicromolar concentrations of Ca2 + , independent of calmodulin . - pubmed
All four pyridine nucleotides stimulated PI - PLC . - pubmed
The diglyceride lipase inhibitor , RHC 80267 , and the cyclooxygenase inhibitor , indomethacin , had no effect on PI - PLC activity . - pubmed
Platelet adhesivity and aggregation induced by ADP , adrenaline and collagen , and malondialdehyde production , are tests in which dipyridamole and aspirin shows a potentiation synergism . - pubmed
Trunk plasma corticosterone ( B ) and plasma T , and B production by collagenase - isolated adrenocortical cells incubated briefly ( 2 hr ) with or without steroidogenic agents were measured by radioimmunoassay . - pubmed
Orchiectomy with implantation of the inert sterol , cholesterol ( hereafter referred to as orchiectomy ) , did not alter plasma B concentrations and did not affect basal cellular B production or cellular B production induced by a maximal steroidogenic concentration of ACTH or that maximally supported by 25 - hydroxycholesterol . - pubmed
Low - implant doses of A4 ( 1 - cm implant ) and T ( 0.3 - cm implant ) , that maintained comb growth , lowered plasma B concentrations by 24 - 42 % , whereas a high - implant dose of T ( 3 - cm implant ) and all implant doses of DHT had no effect on plasma B concentrations . - pubmed
In contrast , androgen replacement consistently suppressed basal and maximal ACTH - induced cellular B production regardless of the type of androgen . - pubmed
The method is based on the fact that the anti - factor VIII antibodies were predominantly of the IgG4 subclass ; anti - human IgG4 antibodies were covalently linked to agarose and large amounts of anti - factor VIII antibodies can be eliminated . - pubmed
Xylosyltransferase activity was determined in synovial fluid and serum by a radiochemical method , based on the incorporation of [ 14C ] xylose from UDP -[ 14C ] xylose into an exogenous acceptor protein . - pubmed
BCNU and CDDP were least active in the monolayer assay , whereas HME showed both the lowest ID90 and steepest slope using this technique . - pubmed
The MK-0787 / MK-0791 is a combination of imipenem ( a carbapenem antibiotic ) and cilastatin sodium ( a dehydro peptidase - I inhibitor ) in a 1 to 1 ratio , which produces a higher urinary recovery of imipenem than imipenem alone . - pubmed
The determination of the saturation of serum albumin with bilirubin . - pubmed
Maternal and umbilical cord serum were checked for copper and zinc by the proton - induced X - ray emission ( PIXE ) method ; maternal serum protein , hemoglobin and estriol were determined as well . - pubmed
Relaxation to histamine was potentiated by inhibition of histamine H1 - receptors with chlorpheniramine . - pubmed
Effects of Ca2 + and Mg2 + on the actin - activated ATP hydrolysis by phosphorylated heavy meromyosin from arterial smooth muscle . - pubmed
Actin - activated ATP hydrolysis by phosphorylated arterial myosin is Ca2 + -dependent at Mg2 + concentrations which allow myosin to bind 2 mol of Ca2 + per mol ( S. Chacko and A. Rosenfeld ( 1982 ) Proc . - pubmed
The possible effects of myosin filament formation and conformational change involving the rod portion of the myosin molecule on actin - myosin interaction and actin - activated ATP hydrolysis was eliminated by utilizing soluble heavy meromyosin ( HMM ) . - pubmed
Actin - activated ATPase activity of the phosphorylated HMM was measured either at constant Mg2 + and variable Ca2 + or at constant Ca2 + and variable Mg2 + concentrations . - pubmed
At constant ( 0.05 M ) ionic strength and pCa 5 , the actin - activated ATPase activity increased until the free Mg2 + reached between 0.5 - 1 mM ; this was followed by a decrease in activity to a very low level at 7 mM free Mg2 + . - pubmed
Removal of Ca2 + at 1 mM free Mg2 + lowered the actin - activated ATPase activity ( 40 - 60 % inhibition ) . - pubmed
These experiments demonstrated that Mg2 + and Ca2 + had a direct effect on actin - activated ATP hydrolysis . - pubmed
It is concluded that combined treatment with glucocorticoid and beta 2 - adrenoceptor agonist may result in a synergistic interaction on severe airway obstruction without significant influence on cardiovascular system . - pubmed
Thymopoietin ( originally termed TP-5 and now called thymopentin ) is a synthetic pentapeptide that can reproduce the biological activity of the 49 amino acid thymic hormone thymopoietin . - pubmed
Treatment of plasma with specific enzyme inhibitors followed by incubation with thymopentin or thymopoietin confirmed that serine protease and aminopeptidase M - like enzymes are responsible for the rapid inactivation of thymopentin in plasma , as measured by the electromyographic response . - pubmed
During the first few turnovers of the enzyme catalytic cycle , when an appreciable amount of E approximately P is still present , the observed heat is much smaller than the heat expected from the amount of ATP hydrolyzed . - pubmed
IgG - titers in all ELISA assays were between 10 - fold to 100 - fold higher when formalin - heat inactivated preparations were employed as compared to phenol - heat inactivated . - pubmed
It is different from the binding fo Co2 + , which binds to the base and phosphate of residue G15 . - pubmed
Cimetidine in a low dose of 10 mg given intravenously increases significantly the gastric potential difference ( GPD ) without any inhibitory effect on gastric acid secretion . - pubmed
The increase of GPD after 200 mg cimetidine is higher than after 10 mg . - pubmed
[ Effect of lincomycin , chymotrypsin and their combinations on the splenic plasmacytic reaction and the antibody titer in experimental staphylococcal infection ] . - pubmed
Lincomycin and chymotrypsin were used in doses of 150 and 2 mg / kg respectively . - pubmed
It was shown that the treatment of the experimental staphylococcal infection with lincomycin resulted in decreased proliferation of the plasmatic cells and antibody formation . - pubmed
The use of chymotrypsin in conjunction with lincomycin lowered the suppressing effect of the latter on the above indices . - pubmed
We analysed the effects of morphine microinjections ( 0.2 microliter ) into the subnucleus oralis ( SNO ) , i.e. - pubmed
This effect was dose - dependent , reversed by naloxone , and also site - specific to the SNO since no effect was observed after microinjection of morphine into areas adjacent to the SNO . - pubmed
A hypoplastic cortical plate was induced and histological analysis and BrdU - immunohistochemistry revealed that the RGD sequence is involved in neuronal migration and proliferation in the telencephalon during formation of the cortical plate . - pubmed
The energy provision from glutamine also plays a role in the process of platelet activation , known to require an extra supply of ATP , since thrombin increased the rate of glutamine utilization in platelets . - pubmed
Upon cell activation an increase in aspartate and CO2 formation was observed and the stimulatory effect on glutamine oxidation by thrombin was also maintained in the presence of glucose . - pubmed
In this way the regulation of hepatic glutaminase expression is similar to that seen for other enzymes involved in hepatic amino acid catabolism but differs markedly from that of renal glutaminase , in which changes in transcription rate are not observed and alterations of mRNA turnover are the principle mechanism of long - term regulation . - pubmed
Gonadal function was monitored by serum LH , FSH , testosterone , SHBG and inhibin levels . - pubmed
In haemophiliacs with less than 1 % factor VIII level , rise was maximum ( 3 - 6 folds ) ; mean factor - VIII level at 7th and 14th day of danazol therapy was 2.3 + /- 0.6 % and 4.8 + /- 1.1 % , respectively . - pubmed
There was a significant positive correlation between the increases in Cx-43 transcripts and the increase in the ratio of plasma estradiol to progesterone . - pubmed
Our data demonstrate : 1 ) that transcripts encoding the gap junction protein , Cx-43 , are at maximal levels during delivery and that this increase is temporally associated with increases in Cx-43 protein and the appearance of gap junctions ; and 2 ) that these data , in association with changes in plasma steroid concentrations , are consistent with myometrial Cx-43 transcript levels being regulated positively by estrogen and negatively by progesterone during pregnancy . - pubmed
Three putative cAMP responsive elements ( CRE ) and one putative TPA responsive element ( TRE ) were identified upstream of the GC rich region . - pubmed
The results of this study suggest that corrective intertrochanteric osteotomy is a successful treatment for Ficat and Arlet stage - II or III disease if the patient has a small or medium lesion ( a combined necrotic angle of less than 200 degrees ) and is not receiving continuous high doses of corticosteroids . - pubmed
SEB was chemically conjugated to tumor cells using a heterobifunctional cross - linking agent acting through NH2 and SH groups . - pubmed
It is shown that formylphenyl ester of ATP is not the substrate in the reaction of elongation catalyzed by primase . - pubmed
Similarly , interferon alpha-2b did not inhibit the culture of mast cells from CD34 + bone marrow - derived cells in the presence of SCF . - pubmed
Intact Kat4c and F ( ab ') 2 fragments were found to augment fMLP - induced oxidation of 1,2,3 - dihydrorhodamine ( oxidant species production ) and beta 2 integrin - mediated adhesion to fibrinogen but did not promote beta 2 integrin - mediated binding of albumin coated latex beads . - pubmed
Biochemical and antiproliferative properties of 4 -[ ar ( alk ) ylamino ] pyridopyrimidines , a new chemical class of potent and specific epidermal growth factor receptor tyrosine kinase inhibitor . - pubmed
The tyrosine kinase inhibitors PD 69896 , 153717 , and 158780 , which belong to the chemical class 4 -[ ar ( alk ) ylamino ] pyridopyrimidines , have been characterized with respect to enzymology , target specificity , and antiproliferative effects in tumor cells . - pubmed
PD 158780 reduced clone formation in soft agar of fibroblasts transformed by EGF , EGF receptor , or the neu oncogene but not ras or raf , further demonstrating its high degree of specificity . - pubmed
The sechiumin cDNA has 951 nucleotides , encoding a protein with 285 amino acids . - pubmed
Two amino acid residues , Glu160 and Arg163 , at the putative active site of sechiumin , are known to be catalytically active in ricin and abrin . - pubmed
Submicellar bile salts stimulate phosphatidylcholine transfer activity of sterol carrier protein 2 . - pubmed
To explore a potential role for sterol carrier protein 2 ( SCP2 , also known as non - specific lipid transfer protein ) in hepatocellular phospholipid trafficking , we examined the influence of submicellar bile salt concentrations on phosphatidylcholine ( PC ) transfer activity of SCP2 . - pubmed
Taurine- and glycine - conjugated bile salts ( anionic steroid detergent - like molecules ) , at concentrations well below their critical micellar concentrations , stimulated PC transfer activity of SCP2 80- to 140 - fold . - pubmed
Thermodynamic and kinetic analyses of PC transfer support a mechanism in which bile salts stimulate SCP2 activity by partitioning into donor vesicles and enhancing membrane association of SCP2 . - pubmed
Myocardial blood flow ( MBF ) was measured during dipyridamole loading and at baseline using positron emission tomography and 13N - ammonia , after which MVD was calculated before and after lipid - lowering therapy . - pubmed
There was a significant relationship between the percent change of total cholesterol and the percent change of MVD before and after lipid - lowering therapy ( r=-0 . - pubmed
Gas chromatography ( GC ) is used for the analysis of trans - fatty acids in partially hydrogenated vegetable oils . - pubmed
The isolated trans - monoenes are quantitatively analyzed by capillary GC . - pubmed
The technique of argentation TLC with GC analysis of isolated methyl esters is highly reproducible with 4.8 % variation ( i.e. - pubmed
The mutation is located in the S4 voltage sensor of domain IV , predicting substitution of histidine for the fifth of eight arginines ( R1460H in hSkm1 ) . - pubmed
However , there was no increase in persistent sodium current as observed for SCN4A mutations causing myotonia or periodic paralysis . - pubmed
The present review deals with various aspects of such regulations , emphasizing the role of calcium / calmodulin in activating AC1 and AC8 in the central nervous system , the potential inhibitory effect of calcium on AC5 and AC6 , and the changes in the expression pattern of the isoforms during development . - pubmed
Spectroscopy of polymer / drug formulations processed with supercritical fluids : in situ ATR - IR and Raman study of impregnation of ibuprofen into PVP . - pubmed
ATR - IR spectroscopy has also revealed specific interactions between the C = O groups of PVP and CO2 . - pubmed
Fludarabine ( FLU , a fluorinated purine analog ) and idarubicin ( IDA , a DNA - topoisomerase II poison ) are frequently used in cancer chemotherapy . - pubmed
Changes in respiratory rate ( RR ) , heart rate ( HR ) , oxygen saturation ( SpO(2 ) ) , Respiratory Distress Assessment Instrument ( RDAI ) , and activity levels were assessed at 20 minutes intervals at times -20 , 0 , 20 , 40 , and 60 minutes around treatment . - pubmed
casseliflavus and 47/47 vancomycin susceptible enterococci were negative for the vanA and vanB genes in both PCR assays . - pubmed
Abietadiene synthase from grand fir catalyzes two sequential , mechanistically distinct cyclizations , of geranylgeranyl diphosphate and of copalyl diphosphate , in the formation of a mixture of abietadiene isomers as the committed step of diterpenoid resin acid biosynthesis . - pubmed
To obtain an insight into fluorophores formed in proteins during lipid peroxidation , a lysine residue analogue ( N ( alpha )- hippuryllysine ) was exposed to autoxidation of linoleic acid catalyzed by iron ( III )- EDTA and L - ascorbic acid . - pubmed
The reaction predominantly produced two fluorescent products , N , N '- bis [ 5 -( N - benzoylglycylamino )- 5 - carboxypentyl ]- 2 - hydroxy-2 - pentyl-3 - imino - l,2 - dihydropyrrole ( II ) and N , N '- bis [ 5 -( N - benzoylglycylamino )- 5 - carboxypentyl ]- 2 - hydroxy-2 -( 7 - carboxyheptyl )- 3 - imino-1,2 - dihydropyrolle ( I ) . - pubmed
Studies on serum oxidase ( ceruloplasmin ) inhibition by tryptophan metabolities . - pubmed
The appearance of antibody of higher specific activity was demonstrated to be a specific response to the antigen which was reinjected . - pubmed
In term of carbohydrate ( CHO ) , the ratio of slow digestible starch ( SDS ) and resistant starch ( RS ) to total CHO for two biscuits ( & gt ; 60 % ) were higher than bread ( 18.3 % ) , although the sugar content were also higher . - pubmed
Contrast to GI and II value of glucose ( as 100 % ) , the GI and II for bread was 96.2 % and 105.2 % , respectively . - pubmed
Glucocorticoid ( GC ) sensitivity was measured by dexamethasone ( DEX ) inhibition of lipopolysaccharide ( LPS)-induced interleukin-6 ( IL-6 ) and tumor necrosis factor - alpha ( TNF - alpha ) production in whole blood . - pubmed
We compared trysin - digested protein samples desalted by ZipTip(C18 ) reverse - phase microcolumns with on - plate washing of peptides deposited either on paraffin - coated plates ( PCP ) , Teflon - based AnchorChip plates , or stainless steel plates , before analysis by matrix - assisted laser desorption / ionization - time of flight - mass spectrometry ( MALDI - TOF - MS ) . - pubmed
We demonstrate that the method of washing of protein samples on paraffin - coated plates provides an easy , reproducible , inexpensive , and high - throughput alternative to ZipTip(C18)-based purification of protein prior to MALDI - TOF - MS analysis . - pubmed
Oral ( OGTTs ) and intravenous glucose tolerance tests ( IVGTTs ) and a euglycaemic hyperinsulinaemic clamp were performed before and at 6 and 12 months of treatment . - pubmed
In OGTTs , serum insulin and C - peptide concentrations 2 h after the oral glucose load were increased ( P<0.001 and P=0.05 respectively ) at 12 months of treatment with TIB , but no changes in the areas under the curve ( AUC ) of insulin or C - peptide were observed . - pubmed
CONCLUSIONS : Despite the impairment in peripheral insulin sensitivity , TIB treatment had a neutral effect on glucose tolerance , possibly due to a compensatory decrease in endogenous glucose production . - pubmed
The increased demand on insulin induced by ED , due to both a stimulatory effect on pancreatic beta cells and increased insulin metabolism , may explain the slightly detrimental effect on glucose tolerance with this treatment . - pubmed
Oximes - induced reactivation of rat brain acetylcholinesterase inhibited by VX agent . - pubmed
Insulin - secreting beta - cells , located within the pancreatic islets of Langerhans , are excitable cells that produce regular bursts of action potentials when stimulated by glucose . - pubmed
Neonates of & lt;33 weeks GA with MUL showed significantly increased PTS at term . - pubmed
In a systematic screen of brain - expressed genes from this region in 210 families with XLMR , we identified seven different mutations in JARID1C , including one frameshift mutation and two nonsense mutations that introduce premature stop codons , as well as four missense mutations that alter evolutionarily conserved amino acids . - pubmed
[ The dynamics of the domains of the IP3 - binding site of the inositol-1,4,5 - triphosphate - sensitive calcium channel , induced by inositol-1,4,5 - triphosphate and calcium ] . - pubmed
The dynamics of the inositol-1,4,5 - triphosphate - sensitive calcium channel after binding of inositol-1,4,5 - triphosphate and Ca2 + was analyzed by the Monte Carlo minimization technique . - pubmed
In the study we explored ( 1 ) the effect of the length of acyl chains of lipids ( C16:1 - C24:1 ) and alkanes ( C6 - C16 ) , ( 2 ) the role of the carbonyl group of lipids , and ( 3 ) the effect of molecular structure of the sarcoplasmic reticulum membrane on TeCP partitioning . - pubmed
present in vivo evidence for 2 important functions of CHGA : the regulation of catecholamine - containing dense - core chromaffin granule biogenesis in the adrenal gland and the control of blood pressure . - pubmed
These results suggest a coupled relationship between CHGA - mediated chromaffin granule biogenesis , necessary for catecholamine storage , and catestatin - induced inhibition of cholinergic - stimulated catecholamine release , which regulates autonomic control of blood pressure . - pubmed
A quantitative structure - activity relationship study on some aromatic / heterocyclic sulfonamides and their charged derivatives acting as carbonic anhydrase inhibitors . - pubmed
A quantitative structure - activity relationship ( QSAR ) study is made on a series of aromatic / heterocyclic sulfonamides and their charged derivatives acting as carbonic anhydrase ( CA ) inhibitors . - pubmed
2000 ; 43 : 292 ) for the selective inhibition of CAs -- sulfonamides generally do not discriminate between different CA isozymes and hence exhibit many undesirable side effects when used as drugs against a particular disease . - pubmed
Acute estradiol significantly increased the number of surviving CA1 neurons , assessed either at 7 days after ischemia or after the completion of behavioral testing 9 days after ischemia . - pubmed
In contrast , chronic estradiol did not reduce CA1 cell death 9 days after ischemia . - pubmed
In an in vitro study , we also examined the potential role of butyrate in modifying the generation of the profibrotic cytokine transforming growth factor - beta ( TGF - beta1 ) by renal epithelial cells . - pubmed
In vitro work demonstrated that exposure of renal epithelial cells to elevated concentrations of butyrate suppressed both basal and stimulated TGF - beta1 synthesis . - pubmed
The action of butyrate was mediated by suppression of the extracellular signal - regulated kinase / mitogen - activated protein kinase signalling pathway . - pubmed
In addition , butyrate exposures reduced the response of renal epithelial cells to TGF - beta1 as assessed by luciferase activity of a TGF - beta - responsive reporter construct . - pubmed
In conclusion , the data demonstrate that dietary supplementation with SUPERGU increased serum butyrate , which at least in vitro has beneficial effects on renal pro - fibrotic cytokine generation . - pubmed
Whereas all treatments affected levels of GH mRNA , only CORT , GHRH , and SRIF significantly altered GHRH - R mRNA levels . - pubmed
Furthermore , there was no change in the colocalization of EphA2 and VE - cadherin at cell - cell adhesion sites when EphA2 was phosphorylated on tyrosine residues . - pubmed
The inhibitors of the mitochondrial ATPase , oligomycin and azide , and also rotenone and antimycin A , were all without effect . - pubmed
Our results indicate that elemental semiconductors from group 14 of the periodic table can be made to adopt mesostructured forms such as MSU - Ge-1 , which features two three - dimensional labyrinthine tunnels obeying Ia3d space group symmetry and separated by a continuous germanium minimal surface that is otherwise amorphous . - pubmed
The depletion of platelet GSH and the increase in S - glutathionylated proteins occurred at concentrations of disulfiram that inhibited platelet aggregation , suggesting that protein S - glutathionylation is involved in the inhibition of platelet aggregation caused by disulfiram . - pubmed
BACKGROUND : Few data are currently available investigating neurosteroids ( NS ) in Alzheimer ' s disease ( AD ) . - pubmed
subtilis mutator strain ( mutS , mutM , mutY ) , two thermo - adaptants were isolated from the groEL substituted strain at 52 degrees C. They contained novel amino acid alterations in their ATP binding motif ( T93I ) and the inter - monomer contact ( R285H ) region of GroEL . - pubmed
The presented simulation algorithm allows to model two experimentally observed instances of the metal oxide / electrolyte interface : one possessing a common intersection point ( CIP ) at pH = PZC and the other one with CIP not equal PZC . - pubmed
Olmesartan prevented vascular eNOS dimer disruption or the downregulation of dihydrofolate reductase ( DHFR ) more than pravastatin , whereas Akt phosphorylation was enhanced by pravastatin but not olmesartan , indicating differential pleiotropic effects between olmesartan and pravastatin . - pubmed
Moreover , pravastatin enhanced the increase in eNOS activity by olmesartan , being associated with additive effects of pravastatin on phosphorylation of Akt and eNOS . - pubmed
Density functional theory ( B3LYP ) and ab initio ( MP2 ) methods with the 6 - 31G(d , p ) basis set are used to study the mechanisms for the hydrolysis of N -( 2 - oxo-1,2 - dihydro - pyrimidinyl ) formamide ( PFA ) in the gas phase . - pubmed
pretreatment with the mu - opioid receptor antagonist beta - funaltrexamine , the kappa - opioid receptor antagonist nor - binaltorphimine and the delta - opioid receptor antagonist naltrindole . - pubmed
pretreatment with antisera against the endogenous kappa - opioid peptides dynorphin A , dynorphin B and alpha - neo - endorphin ; and the endogenous delta - opioid peptide [ Leu(5)]enkephalin . - pubmed
The present results suggest that the spinal antinociception induced by the mu - opioid receptor selective peptide amidino - TAPA is mediated in part by the release of endogenous opioid peptides in the spinal cord , which is caused by the direct stimulation of mu - opioid receptors . - pubmed
Light is thought to be absorbed by mitochondrial chromophores leading to an increase in adenosine triphosphate ( ATP ) , reactive oxygen species and/or cyclic AMP production and consequent gene transcription via activation of transcription factors . - pubmed
A natural material , collagen , was then immobilized onto the nanofiber surface by N -( 3 - dimethylaminopropyl )- N '- ethylcarbodiimide hydrochloride ( EDC)/N - Hydroxysuccinimide ( NHS ) activation process . - pubmed
The stability of the cTnI level was best in the serum , lithium - heparin plasma , and lithium - heparin whole blood samples ( n = 10 , decrease & lt ; 10 % in 24 hours at +20 ( degrees ) C and at +4 ( degrees ) C. There was no remarkable influence of hemodialysis on the cTnI release . - pubmed
A new series of neutral 5 - substituted 4 - anilinoquinazolines as potent , orally active inhibitors of erbB2 receptor tyrosine kinase . - pubmed
Starting from initial lead 1 containing a basic 5 - substituent , optimisation of the glycolamide - derived neutral 5 - substituent led to potent inhibitors of erbB2 with good pharmacokinetics . - pubmed
RESULTS : Based on receiver operating characteristic ( ROC ) curve analysis , the CCCR was only marginally better , as judged by the area under curve ( AUC = 0.923 + /- 0.021 ( SE ) ) , than the 24 - h calcium / creatinine excretion ratio ( AUC = 0.903 + /- 0.027 ) and the 24 - h calcium excretion ( AUC = 0.876 + /- 0.029 ) . - pubmed
Glial - gonadotrophin hormone ( GnRH ) neurone interactions in the median eminence and the control of GnRH secretion . - pubmed
This function , initially described for astrocytes apposing magnocellular neurones , has been more recently characterised for neurones secreting gonadotrophin hormone - releasing hormone ( GnRH ) . - pubmed
Last , an isotopic labeling technique applied to the rhodamine 6 G ( R6G )/ silver system serves as an additional proof of the existence of single - molecule SERS and explores the dynamical features of this process . - pubmed
The ST6Gal - I glycosyltransferase , which adds alpha2 - 6 - linked sialic acids to glycoproteins , is overexpressed in colon adenocarcinoma , and enzyme activity is correlated with tumor cell invasiveness . - pubmed
Correspondingly , collagen binding is reduced by enzymatic removal of cell surface sialic acids from ras - expressors with high ST6Gal - I levels ( i.e. - pubmed
Improvement of charge - transfer indices for multifunctional amino acids : application to lysozyme . - pubmed
The use of CT / valence CT indices { G(k ) , J(k ) , G(k)(V ) , J(k)(V ) } gives limited results for modelling amino acid pI. - pubmed
The fitting line for 21 amino acids is used to estimate lysozyme pI replacing ( 1 + Delta n / n(T ) ) with ( M + Delta n)/n(T ) . - pubmed
The increase in [ Ca(2+)](i ) of both cultures was inhibited by pyridoxalphosphate-6 - azophenyl-2 ' , 4 '- disulphonic acid , a P2 receptor antagonist . - pubmed
U73122 , phospholipase C inhibitor and Thapsigargin , a calcium - pump inhibitor , significantly inhibited the increase in [ Ca ( 2 +)]( i ) at both stages . - pubmed
CbpF shows a new modular structure composed both of consensus and non - consensus choline - binding repeats , distributed along its length , which markedly alter its shape , charge distribution and binding ability , and organizing the protein into two well - defined modules . - pubmed
Effect of different application depths of mitomycin - C in deep sclerectomy with collagen implant : a randomized controlled trial . - pubmed
PURPOSE : To evaluate effects of mitomycin - C ( MMC ) in deep sclerectomy with collagen implant applied under the superficial scleral flap or under the deep scleral flap . - pubmed
Randomized controlled trials ( RCTs ) on the treatment of FMS with GPT and PGB were analyzed . - pubmed
Intermittent hypoxia - mediated plasticity of acute O2 sensing requires altered red - ox regulation by HIF-1 and HIF-2 . - pubmed
This article provides a brief review of recent studies addressing the effects of chronic intermittent hypoxia ( IH ) on acute O2 sensing in carotid bodies ( CBs ) and adrenal medullary chromaffin cells ( AMCs ) and the underlying mechanisms . - pubmed
Thus , the studies with chronic IH suggest novel , hitherto uncharacterized , roles for HIF-1 and HIF-2 in regulating red - ox status leading to plasticity of O2 sensing in CBs and AMCs . - pubmed
Mg2+/Ca2 + cation binding cycle of guanylyl cyclase activating proteins ( GCAPs ): role in regulation of photoreceptor guanylyl cyclase . - pubmed
Suppression of Ca ( 2 + ) influx through the cGMP channel by light activates retinal guanylyl cyclase through guanylyl cyclase activating proteins ( GCAPs ) and thus expedites photoreceptors recovery from excitation and restores their light sensitivity . - pubmed
GCAP1 and GCAP2 , two ubiquitous among vertebrate species isoforms of GCAPs that activate retGC during rod response to light , are myristoylated Ca ( 2 +)/ Mg ( 2 +)-binding proteins of the EF - hand superfamily . - pubmed
In the metal binding EF - hands of GCAP1 , different point mutations can selectively block binding of Ca ( 2 + ) or both Ca ( 2 + ) and Mg ( 2 + ) altogether . - pubmed
Activation of retGC at low Ca ( 2 + ) ( light adaptation ) or its inhibition at high Ca ( 2 + ) ( dark adaptation ) follows a cycle of Ca ( 2 +)/ Mg ( 2 + ) exchange in GCAPs , rather than release of Ca ( 2 + ) and its binding by apo - GCAPs . - pubmed
The Mg ( 2 + ) binding in two of the EF - hands controls docking of GCAP1 with retGC1 in the conditions of light adaptation and is essential for activation of retGC . - pubmed
Mg ( 2 + ) binding in a C - terminal EF - hand contributes to neither retGC1 docking with the cyclase nor its subsequent activation in the light , but is specifically required for switching the cyclase off in the conditions of dark adaptation by binding Ca ( 2 + ) . - pubmed
The Mg ( 2 +)/ Ca ( 2 + ) exchange in GCAP1 and 2 operates within different range of intracellular Ca ( 2 + ) concentrations and provides a two - step activation of the cyclase during rod recovery . - pubmed
Additionally , alcohol - dependent patients were interviewed with the Alcohol Use Disorders Identification Test ( AUDIT ) and were subtyped according to Lesch 's Alcohol Typology ( LAT ) . - pubmed
There were no significant gender differences in the AUDIT and LAT between alcohol - dependent men and women . - pubmed
Because VEGF and its downstream mediator nitric oxide have a well - established cardioprotective role , however , it can be argued that the beneficial effects of VEGF antagonism in the eye may come at the cost of adverse systemic effects , particularly myocardial infarction and stroke . - pubmed
CONCLUSION : Results suggest the combination of a PBI and a peer - delivered BMI is an appropriate and efficacious way to reduce drinking and related consequences among individuals who initiated drinking earlier in adolescence and are at an increased risk of experiencing alcohol problems . - pubmed
OxyVita - zero - link polymerized hemoglobin ( OxyVita Hb ) is a novel generation hemoglobin based oxygen carrier ( HBOC ) . - pubmed
The enzyme homogentisate phytyltransferase ( HPT ) performs the first committed step of the vitamin E biosynthetic pathway . - pubmed
The cDNA , designated as LsHPT , was 1,670 bp long containing an open reading frame ( ORF ) of 1,185 bp which encoded a protein of 395 amino acids . - pubmed
Transient expression of LsHPT via agroinfiltration resulted in 9 - fold increase in LsHPT mRNA level and nearly 18 - fold enhancement in alpha - tocopherol content compared with the negative controls . - pubmed
Our research showed that concentrations of lactate , phosphates and pH value post - mortem depended on the perimortal situations . - pubmed
Next , we use these novel inhibitors to pinpoint the time of Hog1 action during recovery from G(1 ) checkpoint arrest , providing further evidence for a specific role of Hog1 in regulating cell cycle resumption during arsenite stress . - pubmed
RESULTS : We have developed an improved kinetic model featured with the incorporation of butyryl - phosphate , inclusion of net effects of complex metabolic regulations , and quantification of endogenous enzyme activity variations caused by these regulations . - pubmed
Through parameter perturbation analysis , it was found that butyrate kinase has large and positive influence on butanol production while CoA transferase has negative effect on butanol production , suggesting that butyrate kinase has more efficiency in converting butyrate to butanol than CoA transferase . - pubmed
This clinical evidence leads to the hypothesis that GSH conjugation catalyzed by GSTT1 and GSTM1 has a role in the elimination of reactive metabolites of troglitazone . - pubmed
It is of interest that one of the reactive metabolites with a quinone structure was predominantly conjugated with GSH by GSTM1 . - pubmed
Thus , we demonstrated that the GST isoforms contributed differently to the GSH conjugation of individual reactive metabolites of troglitazone , and GSTM1 is the most important GST isoform in the GSH conjugation of a specific reactive metabolite produced from the cytotoxic , quinone - form metabolite of troglitazone . - pubmed
Finally , cerebral phospho - ERK expression was measured by western blot . - pubmed
Furthermore , the elevated caspase-3 activity seen following burn injury was remarkably reduced by high dose gelsolin treatment along with down - regulation of phospho - ERK expression . - pubmed
Idraparinux is a polymethylated synthetic pentasaccharide that binds to antithrombin with high affinity . - pubmed
By studying the expression of ER specific hallmarks , such as GRP78 , GRP94 and GADD153 / CHOP , we found a possible pivotal role of ER signaling modulation in the crosstalk between autophagy and apoptosis induced by cisPt . - pubmed
To the best of our knowledge , this is the first demonstration that taurine enhances autophagic protection against apoptosis by reducing ER stress , thus making it possible to develop new strategies to reduce severe cisPt - induced side - effects such as nephrotoxicity . - pubmed
Treg and IL-6 levels decreased following GEM monochemotherapy , IL-17A levels decreased after GEMOX , and IL-6 levels were reduced subsequent to BEV+CAPE+RT treatment . - pubmed
A regulatory path associated with X - linked intellectual disability and epilepsy links KDM5C to the polyalanine expansions in ARX . - pubmed
Polyalanine ( polyA)-expansion - encoding mutations of aristaless - related homeobox ( ARX ) cause a spectrum of X - linked ID ( XLID ) diseases and chronic epilepsy , including infantile spasms . - pubmed
In this study , the effects of Rho - inactivating toxins on the concentrations of cellular F - actin were investigated using the rhodamine - phalloidin - based F - actin ELISA . - pubmed
The immunosuppressive agent mizoribine monophosphate is an inhibitor of the human RNA capping enzyme . - pubmed
Mizoribine monophosphate ( MZP ) is a specific inhibitor of the cellular inosine-5 '- monophosphate dehydrogenase ( IMPDH ) , the enzyme catalyzing the rate - limiting step of de novo guanine nucleotide biosynthesis . - pubmed
MZP is a highly potent antagonistic inhibitor of IMPDH that blocks the proliferation of T and B lymphocytes that use the de novo pathway of guanine nucleotide synthesis almost exclusively . - pubmed
In the present study , we investigated the ability of MZP to directly inhibit the human RNA capping enzyme ( HCE ) , a protein harboring both RNA 5 '- triphosphatase and RNA guanylyltransferase activities . - pubmed
Our biochemical studies provide the first insight that MZP can inhibit the formation of the RNA cap structure catalyzed by HCE . - pubmed
In the presence of MZP , the RNA 5 '- triphosphatase activity appears to be relatively unaffected while the RNA guanylyltransferase activity is inhibited , indicating that the RNA guanylyltransferase activity is the main target of MZP inhibition . - pubmed
Kinetic studies reveal that MZP is a non - competitive inhibitor that likely targets an allosteric site on HCE . - pubmed
RESULTS : On the basis of this evidence we selected Rifampicin , a bacterial RNA polymerase inhibitor , and then AMBER 12 was used to simulate the behavior of the RPO - Rifampicin complex after a set of 5000 ps and up to 300 K in water . - pubmed
The second group contained 40 teeth , restored with pure titanium casting post and core . - pubmed
pylori strains 26695 and SS1 and followed a sequential linear assembly of the alpha-1,6 - glucan , dd - heptan , and O - chain components . - pubmed
Cat , Gpx7 , Gad1 , Hspa12A , Foxn1 , adenosine A1 receptor , Ca ( 2 + ) adaptor and homeostasis proteins , Cacnb4 , Atp2b2 , anti - apoptotic Bcl-2 gene members , intracellular trafficking protein , Grasp and suppressor of cytokine signaling ( Socs3 ) ] . - pubmed
The observed differential transcriptional responses may contribute to early - life seizure - induced pre - conditioning and neuroprotection by reducing glutamate receptor - mediated Ca ( 2 + ) permeability of the hippocampus and redirecting inflammatory and apoptotic pathways . - pubmed
Potassium iodide , systemic and intralesional corticosteroids , non - steroidal anti - inflammatory drugs , salicylates , tumor necrosis factor - alpha inhibitors , hydroxychloroquine , colchicine , and dapsone are other treatment options available , but some of these drugs are contraindicated in pregnancy while others are considered safe . - pubmed
The tumors were diagnosed as HCC by typical imaging findings and elevated serum alpha - fetoprotein ( AFP , 1,825.0 ng / ml ) and protein induced by vitamin K absence II ( PIVKA II , 3,043 mAU / ml ) . - pubmed
The effect of Sirt3 on cell proliferation was observed with Edu ( 5 - ethynyl - 2 '- deoxyuridine ) kit after Sirt3 silencing by small interference RNA . - pubmed
Interestingly , unlike MECP , EFCP and AFCP did not have any effect at lower concentrations ( & lt;40microg / ml ) but induced pore opening at 60microg / ml , 80microg/ ml , 100microg / ml and 120microg / ml by 1.6 , 3.1 , 12.7 , 16.7folds , respectively for EFCP and 1.4 , 5.4 , 7 and 10 folds respectively , for AFCP . - pubmed
The effects of magnesium sulfate on placental vascular endothelial growth factor expression in preeclampsia . - pubmed
OBJECTIVE : To evaluate the effect of magnesium sulfate ( MgSO ( 4 ) ) on placental expression levels of vascular endothelial growth factor ( VEGF ) . - pubmed
Exposure of preeclamptic placentas to MgSO ( 4 ) resulted in decreased VEGF levels by the amnion ( 57 + - 26 pg / g protein ) , as compared with the control group ( 153 + - 62 pg / g protein ) ( p & lt ; 0.05 ) . - pubmed
On the other hand , MgSO ( 4 ) significantly increased VEGF levels by the placental villous and the decidua ( 58 + - 15 pg / g protein , 70 + - 29 pg / g protein ; respectively ) , as compared with the control group ( 29 + - 11 pg / g protein , 33 + - 14 pg / g protein ; respectively ) ( p & lt ; 0.01 , p & lt ; 0.05 ; respectively ) . - pubmed
Exposure to MgSO ( 4 ) did not affect VEGF levels in normotensive placentas . - pubmed
Perfusion with MgSO ( 4 ) affects VEGF expression differently by preeclamptic and normotensive placentas . - pubmed
Increased production of placental VEGF in preeclampsia may play a role in the therapeutic action of MgSO ( 4 ) . - pubmed
This study was performed to investigate the effects of acteoside on various cellular functions such as , intracellular Ca ( 2 + ) mobilization , phospholipase C activity , and exocytosis induced by melittin . - pubmed
Melittin ( 0.1 - 1 muM ) dose - dependently increased intracellular Ca ( 2 + ) mobilization in the presence of extracellular Ca ( 2 + ) , but was not affected by 1 muM U73122 , a specific PLC inhibitor . - pubmed
Correlating cation ordering and voltage fade in a lithium - manganese - rich lithium - ion battery cathode oxide : a joint magnetic susceptibility and TEM study . - pubmed
[ Effect of integrin beta8 on TGF - beta1 activation in astrocytes with oxygen glucose deprivation ] . - pubmed
OBJECTIVE : To study the effect of beta8 expression on transforming growth factor beta1(TGF - beta1 ) activation in astrocytes with oxygen glucose deprivation ( OGD ) . - pubmed
Online HILIC / mass spectrometry ( MS ) with a quadrupole time - of - flight mass spectrometer provides accurate mass identifications of the separated , 2 - AB - labeled N - glycans . - pubmed
The use of TFA provided distinct advantages over conventional ammonium formate as a mobile phase additive , such as , optimal elution order for sialylated N - glycans , reproducible chromatographic profiles , and matching total ion current chromatograms , as well as minimal signal splitting , analyte adduction , and fragmentation during HILIC / MS , maximizing sensitivity for trace - level species . - pubmed
Application of this HILIC / MS method to the detailed characterization of a marketed therapeutic mAb , Rituxan ( ) , is described . - pubmed
Inhibition of autophagy potentiates the efficacy of Gli inhibitor GANT-61 in MYCN - amplified neuroblastoma cells . - pubmed
However , NB with MYCN amplification is less sensitive to GANT-61 than those without MYCN amplification . - pubmed
METHODS : Autophagic process was examined in two MYCN amplified and two MYCN non - amplified NB cells treated with GANT-61 . - pubmed
RESULTS : Here we show that GANT-61 enhanced autophagy in MYCN amplified NB cells . - pubmed
Both an autophagic inhibitor 3 - methyladenine ( 3 - MA ) and genetic disruption of ATG5 or ATG7 expression suppressed GANT-61 induced autophagy and significantly increased apoptotic cell death , whereas pre - treatment with an apoptotic inhibitor , Z - VAD - FMK , rescued GANT-61 induced cell death and had no effect on the autophagic process . - pubmed
In the other hand , GANT-61 barely induced autophagy in MYCN non - amplified NB cells , but overexpression of MYCN in MYCN non - amplified NB cells recapitulated GANT-61 induced autophagy seen in MYCN amplified NB cells , suggesting that the level of GANT-61 induced autophagy in NB cells is related to MYCN expression level in cells . - pubmed
To define the functional role of DES1 and the role that the sulfide molecule may play in the regulation of physiological processes in plants , we studied the localization of the expression of this gene at the tissue level . - pubmed
An increase in DES1 expression in response to ABA was recently demonstrated ; in the present work , we observe that in vitro auxin treatment significantly repressed the expression of DES1 . - pubmed
Since aggregation due to contaminating nucleic acids may have occurred during the sample concentration step , anion - exchange column chromatography was performed immediately after affinity chromatography , to separate the intact AdipoR1Delta88 from the aggregating species . - pubmed
The purified AdipoR1Delta88 and AdipoR2Delta99 proteins were characterized by thermostability assays with 7 - diethylamino-3 -( 4 - maleimidophenyl )- 4 - methyl coumarin , thin layer chromatography of bound lipids , and surface plasmon resonance analysis of ligand binding , demonstrating their structural integrities . - pubmed
Differential effects of oxycodone , hydrocodone , and morphine on the responses of D2 / D3 dopamine receptors . - pubmed
Here , we report that TUBB3 directly interacted with another Netrin-1 receptor Down syndrome cell adhesion molecule ( DSCAM ) and Netrin-1 increased this interaction in primary neurons . - pubmed
It is suggested that Ca2 + signal transduction and redox seem to be involved in differentiation , while PGA and EIF are associated with the division of cambium initials for COR - induced secondary laticifer differentiation in the rubber tree . - pubmed
The immobilization relies on the ultra high affinity of calmodulin to N5A mutant CBP ( RWKKNFIAVSAANRFKKIS ) in presence of calcium ( KD~2 pM ) , which can be reversed by EDTA allowing controlled " capture and release " of the specific binders . - pubmed
The MC calculated photon fluence in detector arrays was used for image reconstruction of a simple water phantom as well as polyacrylamide polymer gel ( PAG ) used for radiation therapy . - pubmed
Lck association with CRIF1 in Jurkat mitochondria was confirmed biochemically and by microscopy , but did not lead to CRIF1 tyrosine phosphorylation . - pubmed
AIM : Decreasing cholesterol levels in Western populations is the main reason for decreasing mortality due to coronary heart disease . - pubmed
Blocking the PI3K - Akt pathway by LY294002 led to an enhancement of IL-12 production . - pubmed
PI3 K signalling pathway appears to be involved in the decreased IL-12 secretion by HBeAg treated DCs . - pubmed
By analyzing different variables of the formulation , sodium hydrogen carbonate is introduced as pH modifier to improve the release of GLZ , while ethyl cellulose acts as release retardant to reduce the burst release phase of MTF . - pubmed
Data also shows EOP tablet is able to release MTF and GLZ in a synchronized and sustained manner both in vitro and in vivo . - pubmed
Expression of both the gr1 and gr2 genes was induced by reactive oxygen species . - pubmed
Additionally , when the anterograde neurotracer biotinylated dextran amine ( BDA ) was deposited in the dmVMH subsequent stimulation of the dmVMH produced BDA - labelled neural fibres with terminal boutons surrounding Fos - labelled neurons in the dPAG , suggesting synaptic contacts between dmVMH and dPAG neurons for eliciting panic - like behavioural responses . - pubmed
The commonest clinically significant major resistance mutations associated with the highest levels of reduced susceptibility or virological response to the relevant Nucleoside Reverse Transcriptase Inhibitor ( NRTI ) were ; the Non - thymidine analogue mutations ( Non - TAMS ) M184V-20.7 % and K65R-8.0 % ; and the TAMs M41L and K70R ( both 8.0 % ) . - pubmed
Of the 7 patients with major PI DRMs , five had high level resistance to ritonavir boosted Lopinavir and Atazanavir , with Darunavir as the only susceptible PI tested . - pubmed
An extension to the build - up method and an analysis of the post - exercise recovery period that can increase CO2 generation rates are presented . - pubmed
RESULTS : Supplementation of mothers with 4400 IU of vitamin D3 resulted in an enhanced broad - spectrum proinflammatory cytokine response of cord blood mononuclear cells to innate and mitogenic stimuli ( P = .0009 ) , with an average 1.7- to 2.1 - fold increase in levels of several proinflammatory cytokines ( GM - CSF , IFN - gamma , IL-1beta , IL-6 , and IL-8 ) across stimuli , a higher gene expression level of TLR2 ( P = .02 ) and TLR9 ( P = .02 ) , a greater than 4 - fold increase in IL-17A ( P = .03 ) production after polyclonal T - cell stimulation , and an enhanced IL-10 response of cord blood mononuclear cells to dexamethasone treatment in culture ( P = .018 ) . - pubmed
Selenomethionine was subsequently introduced into the crystals to obtain the phases for the MRF DBD structure . - pubmed
Data from Biolog carbon source utilization tests and nucleotide sequence data from 16S and gyrB loci suggested that both pathogenic and non - pathogenic strains were related to , yet distinct from , previously described Xanthomonas species . - pubmed
Dextran sodium sulfate was given either on post - operative day 9 or day 24 to evaluate immune responses in the intestine both immediately following surgery and after a period of healing . - pubmed
Catecholamine doses and SAPS - II scores decreased significantly . - pubmed
Selenium ( Se ) is an essential trace element that is involved in numerous biological processes in the form of a selenoprotein such as iodothyronine deiodinase ( DIO ) . - pubmed
Increases in free T4 levels during Se deficiency were reduced with Se supplementation ; however , neither free T3 nor TSH levels were affected . - pubmed
X - linked hypophosphatemia ( XLH ) , the most common form of inheritable rickets , is caused by inactivation of phosphate - regulating gene with homologies to endopeptidases on the X chromosome ( PHEX ) and leads to fibroblast growth factor ( FGF ) 23 - dependent renal inorganic phosphate ( Pi ) wasting . - pubmed
Fifty - nine elite runners and triathletes were assessed for the following measurements : Th1 , Th2 and lymphocyte phenotyping and plasma levels of cortisol , chemokines , inflammatory cytokines and specific immunoglobulin E ( IgE ) . - pubmed
Ivacaftor is a drug used to treat cystic fibrosis ( CF ) patients carrying specific gating CFTR mutations . - pubmed
In an independent and larger cohort , we aimed to evaluate whether SLC26A9 variants contribute to the variability of the lung phenotype and if they influence the lung response to ivacaftor . - pubmed
Other SLC26A9 SNPs also contributed to the ivacaftor - response . - pubmed
Interindividual variability in lung response to ivacaftor is associated with SLC26A9 variants in French CF patients . - pubmed
This high yielding , alpha - selective process was found to be independent on the nature or configuration [ ( R )- C ( sp3 )- F , ( S )- C ( sp3 )- F ] of the substituent at C2 . - pubmed
With plasma glucose concentrations clamped between 6 and 9 nmol / l , GLP-1 or saline was infused for 240 min during and after a meal . - pubmed
This review is a short survey of the atomistic computational investigations of K+ and Na+ ion channels , focusing on KcsA and several voltage - gated channels from the KV and NaV families , which have garnered many successes and engendered several long - standing controversies regarding the nature of their structure - function relationship . - pubmed
Incidence of VAs , serum levels of malondialdehyde ( MDA ) and superoxide dismutase ( SOD ) , expression of connexin ( Cx43 ) , Bcl-2 , Bax , caspase-3 , tumor necrosis factor ( TNF )- alpha , interleukin-6 ( IL-6 ) , and high mobility group box ( HMGB ) 1 were compared . - pubmed
Compared with the SO group , Cx43 , SOD , and Bcl-2 were decreased , MDA , Bax , caspase-3 , TNF - alpha , IL-6 , and HMGB1 were increased in the MI group , and all the alterations were significantly restrained in the LOMD+AMI group . - pubmed
The charge distribution and electrostatic potential ( ESP ) of 3D - GN@Cu0 further illustrated the distribution and transfer of electrons on the catalyst surface , which predicted a micro - electrolysis - promoted Fenton - like reaction mechanism . - pubmed
Nicorandil , the first clinically applied ATP - sensitive K + channel ( K+ATP ) opener with nitrate property , has demonstrated cardioprotective effects in patients with multiples of heart diseases . - pubmed
CRISPR and stable gene transfer showed that PGC1alpha knockout tubular cells were sensitized to the genotoxic stressor cisplatin whereas transgenic cells were protected . - pubmed
PGC1alpha not only counteracted this effect but also raised basal mitophagy , as did the downstream mediator nicotinamide adenine dinucleotide ( NAD + ) . - pubmed
PGC1alpha did not consistently affect known autophagy pathways modulated by cisplatin . - pubmed
These results uncover an unexpected effect of cisplatin on mitophagy and PGC1alpha 's exquisite reliance on lysosomes for kidney protection . - pubmed
Nine ACE types , childhood relationships and six health outcomes ( early alcohol initiation , problem alcohol use , smoking , drug use , therapy , suicide attempt ) were explored . - pubmed
Serum albumin saturation test based on non - esterified fatty acids imbalance for clinical employment . - pubmed
Since physiologically fatty acids are transported bound to albumin , we propose here a simple and cheap test that consists of albumin isoelectric focusing determination to measure the potential systemic NEFA cytotoxicity . - pubmed
The albumin saturation test can point out serum albumin - NEFA saturation through a cheap assay that could be performed by any care facility . - pubmed
In the group that used DRT plus NPWT , the mean DRT take was 99.8 % , with a mean maturation time of 16.68days and the mean of the skin graft take rate was 89.1 % . - pubmed
Also , the mean Q computed in the left PA ( QLPA - n ) is within 10 % of the mean QLPA - e. Finally , the compliance cMPA - n is computed to be 27 % lower than cMPA - e , while the cLPA - n is computed to be 20.4 % lower than cLPA - e. Importantly , the PA shows significant intra - vascular variation in compliance , with the MPA showing higher overall compliance compared to the LPA ( 3.5 - 4 times ) . - pubmed
RESULTS : NO2 exposure for 75 days was associated with a significant decrease in FEV100 / FVC% , an increased in functional residual capacity ( FRC ) , and histologic evidence of emphysema , moreover ; NO2 exposure led to elevated triglyceride ( TG ) , red blood cell ( RBC ) , hemoglobin ( HGB ) , and hematocrit ( HCT ) levels . - pubmed
NaHS could alleviate the PF decline as possible indicated by an elevation of HDL - C levels and leukocyte . - pubmed
coli O157 : H7 - infected macrophage ; and these effects of DHEA were heightened when the cells were pre - treated with p38 MAPK inhibitor SB203580 . - pubmed
RESULTS : Multivariate analyses revealed that for participants aged 8 to 11 , daily sodium intake was significantly and positively associated with femoral neck BMD ( B = 0.9 x 10- 5 , p = 0.031 ) ; in particular , subgroup analyses by sex found that in male participants aged 8 - 11 , daily total cholesterol intake ( B = 5.3 x 10- 5 , p = 0.030 ) and calcium intake ( B = - 2.0 x 10- 5 , p & lt ; 0.05 ) were significantly associated with femoral neck BMD in a positive and negative manner , respectively , but neither were observed in female participants of this age group . - pubmed
In contrast , daily intakes of vitamin D and magnesium were significantly and positively associated with femoral neck BMD in female participants aged 8 - 11 ( B = 246.8 x 10- 5 and 16.3 x 10- 5 , p = 0.017 and 0.033 , respectively ) . - pubmed
For participants aged 16 to 19 , daily total fat intake was significantly and negatively associated with femoral neck BMD ( B = - 58 x 10- 5 , p = 0.048 ) ; further stratification by sex found that magnesium and sodium intakes were significantly and positively associated with femoral neck BMD only in females of this age group ( B = 26.9 x 10- 5 and 2.1 x 10- 5 , respectively ; both p & lt ; 0.05 ) . - pubmed
fossilis did not increase urea excretion or accumulation during 6 days of ammonia exposure , and lacked detectable carbamoyl phosphate synthetase I or III activity in its liver . - pubmed
Exposure to 30 mmol l-1 NH4Cl led to significant increases in the transcript levels of nkaalpha1b ( on day 6 ) and nkaalpha1c1 ( on day 1 and 3 ) as compared with the control . - pubmed
fossilis is attributable partly to its ability to actively excrete ammonia with the aid of Nka . - pubmed
Design , synthesis , and molecular docking study of new monastrol analogues as kinesin spindle protein inhibitors . - pubmed
A series of 18 3,4 - dihydropyrimidine analogues bearing a 1,2 - methylenedioxybenzene component at position 4 with diverse side chains at positions 5 and 6 was designed and synthesized as inhibitors of the Eg5 kinesin enzyme . - pubmed
A molecular docking study was performed for some demonstrative compounds as well as monastrol docked into the allosteric binding site of the kinesin spindle protein . - pubmed
This systematic review ( SR ) proposed the following question : Can the toxicity of TiO2 nanoparticles be evaluated in a 3D skin model ? - pubmed
In this SR , 7 articles were selected , and it was concluded that the 3D skin model has shown to be promising to evaluate the toxicity of TiO2 NPs . - pubmed
A strategy to limit this degradation was developed and tested for model ECDs based on a sidechain - modified poly ( 3,4 - ethylene dioxythiophene ) ( PEDOT ) derivative and Prussian blue ( PB ) . - pubmed
Copper is essential for many metabolic processes but must be sequestrated by copper chaperones . - pubmed
It is well known that plant copper chaperones regulate various physiological processes . - pubmed
However , functions of copper chaperones in plant nucleus remain largely unknown . - pubmed
CCP harbors a classical MXCXXC Cu binding site ( CBS ) at its N terminus and a nuclear localization signal ( NLS ) at the C terminus . - pubmed
Collectively , our results have identified a putative nuclear copper chaperone required for plant immunity and provided evidence for potential function of Cu in the SA pathway . - pubmed
, angiotensin II , endothelin 1 , aldosterone ) impairs the balance between vasoconstrictors and vasodilators and , in turn , detrimentally impacts vascular and erectile structures . - pubmed
Widely studied mechanisms of action , such as complex I inhibition leading to AMPK activation , have only been observed in the context of supra - pharmacological ( > 1mM ) metformin concentrations which do not occur in the clinical setting . - pubmed
Efficacy measures included changes from baseline in glycated hemoglobin ( A1C ) , fasting plasma glucose ( FPG ) , and body weight . - pubmed
Venous Air Embolism Identified by 18F - Fluorocholine Dual - Phase PET / CT . - pubmed
F - FCH PET / CT with dual time point was performed , acquiring immediately and 60 minutes after F - FCH administration . - pubmed
A method for the determination of oxoglutarate binding to the external face of the inner membrane is described . - pubmed
Recoveries of individual subunits were 75 % ( S1 ) , 70 % ( S2 ) , greater than 90 % ( S3 ) , greater than 90 % ( S4 ) , and 50 % ( S5 ) , as judged by SDS - PAGE and amino acid analysis . - pubmed
Juvenile GM2 gangliosidosis caused by substitution of histidine for arginine at position 499 or 504 of the alpha - subunit of beta - hexosaminidase . - pubmed
Response of rat liver glutaminase to magnesium ion . - pubmed
The activity of rat liver glutaminase from sedimented fractions of freeze - thawed mitochondria is strongly affected by variation in the Mg2 + concentration within the approximate physiological range of activators . - pubmed
A rise in the Mg2 + concentration stimulates glutaminase by increasing the apparent affinity of the enzyme for its positive modifier phosphate . - pubmed
The result is an increase in the apparent affinity of the enzyme for glutamine . - pubmed
With the addition of 4 mM Mg2 + the S0.5 of glutaminase for glutamine decreased from 24 to 13 mM at pH 7.1 , 14 to 9.6 mM at pH 7.4 , and remained unchanged at 8.2 mM at pH 7.7 . - pubmed
Since Mg2 + stimulates glutaminase , as does a rise in pH ( Szweda , L.I. - pubmed
264 , 15357 - 15360 ) , by increasing the apparent affinity of the enzyme for phosphate , it reduces the inhibitory effect of a decrease in pH and/or phosphate concentration over a physiologically relevant range . - pubmed
Increases in heart rate ( HR ) and mean arterial pressure ( MAP ) in response to TMX ( 16.1 - 28.6 m / min ) were similar after icv administration of saline or no treatment . - pubmed
After icv administration of alpha - helical CRF9 - 41 , HR and MAP responses during TMX were significantly attenuated . - pubmed
In addition , TMX - induced elevations of estimated mesenteric vascular resistance and iliac blood flow velocity were blunted after CRF receptor blockade . - pubmed
Fasting blood glucose declined from 12.3 + /- 0.4 to 10.5 + /- 0.7 mmol / L ( p less than 0.05 ) but mean diurnal glucose and glycosylated hemoglobin A1c did not change significantly . - pubmed
The high - density lipoprotein ( HDL ) cholesterol increased after weight reduction ( 0.96 + /- 0.06 vs 1.11 + /- 0.05 mmol / L , p = 0.009 ) . - pubmed
Inhibition of tumor growth is correlated with spermidine depletion and an increase of decarboxylated - S - adenosylmethionine , the aminopropyl donor in the spermidine and spermine synthase reactions . - pubmed
Finally , this study demonstrates the advantage of using prodrugs of delta - MFMO , an inhibitor of ODC , since they possess longer duration of action , higher potency , and in some cases better antitumor efficiency than the parent direct inhibitor of ODC . - pubmed
This phase is characterized by increased FSH and LH and decreased progesterone / estradiol ratio resulting in irregular bleeding , endometrial hyperplasia , polyps , fibromas , mammary dystrophies , disturbance of mood , appetite and thermoregulation . - pubmed
Lastly , the sensitivity of ovarian follicles to FSH and LH is lost with a decline of E2 below 20 pg / ml produced almost exclusively from peripheral conversion from circulating androgens . - pubmed
The beneficial effects of E2 are lost resulting in atrophy of the sensitive tissues , decreased calcium absorption , increased bone resorption , accelerated bone loss and osteoporosis , rise in serum triglycerides , increased VLDL and LDL lipoproteins , increased LDL / HDL cholesterol , a profile which favors atherosclerosis . - pubmed
Local and systemic antifungal prophylaxis with oral amphotericin B plus itraconazole was given throughout the immediate post - transplant period and there was no evidence of recurrent mycosis . - pubmed
Thermotropic behavior of dimyristoylphosphatidylcholine in the presence of cytochrome c oxidase . - pubmed
Chronic clonidine treatment produces desensitisation of post- but not presynaptic alpha 2 - adrenoceptors . - pubmed
Sympathetic hyperactivity following withdrawal of chronic clonidine treatment may be mediated by down - regulation of postsynaptic alpha 2 - adrenoceptors in the CNS . - pubmed
Stimulation of insulin secretion by glucose , the phorbol ester 12 - O - tetradecanoylphorbol 13 - acetate ( TPA ) or the sulphonylurea glibenclamide did not affect these polyamine contents . - pubmed
In accordance with a role for protein kinase C in insulin secretion , TPA stimulated both protein kinase C activity and insulin secretion . - pubmed
Spermidine ( 5 mM ) and spermine ( 1.5 mM ) at concentrations found in islets inhibited protein kinase C stimulated by TPA + phosphatidylserine by 55 % and 45 % respectively . - pubmed
Inhibition of protein kinase C by polyamines showed competition with Ca2 + , and Ca2 + influx in response to glucose or glibenclamide prevented inhibition of insulin secretion by exogenous polyamines at concentrations where they did not affect glucose oxidation . - pubmed
It is suggested that inhibition of protein kinase C by polyamines may be of significance for regulation of insulin secretion in vivo and that Ca2 + influx may function by displacing inhibitory polyamines bound to phosphatidylserine in membranes . - pubmed
Light causes a 4 - -5 - fold decrease in NAT activity in static organ culture , and inhibits melatonin release in flow - through organ culture . - pubmed
Drugs that increase cyclic nucleotide levels in cells ( cholera toxin , RO 20 - 1724 , monobutyryl cyclic AMP , monobutyryl cyclic GMP ) block the NAT decrease by light , whereas high potassium or EGTA do not block this light - induced NAT inactivation . - pubmed
When added to milk casein and heated , histidine appeared to be the most toxic . - pubmed
Even after treated with papain , the heated casein - histidine mixture was still effective to induce gizzard erosion . - pubmed
The IC50 were 30 microM and 29 microM for taurine efflux and 26 and 27 microM for C1 efflux for NMD and NTD , respectively . - pubmed
An analysis of receiver operating characteristic curves revealed that the highest diagnostic accuracies in distinguishing benign from malignant lung diseases were achieved with TPM ( 81 % ) , CYFRA 21 - 1 ( 72 % ) , CEA ( 78 % ) or TPA ( 78 % ) when using cut - off values of 46 Ul-1 , 3.0 micrograms l-1 , 2.0 micrograms l-1 and 75 Ul-1 respectively . - pubmed
The dorsal , median and pontine raphe nuclei as well as the supralemniscal nucleus ( the B9 group ) contained very few double - labelled cells , which comprised a mean of 0.1 - 0.7 % of all serotonin - immunoreactive cells in each of these nuclei . - pubmed
We suggested the name Ep - CAM for this molecule ( Litvinov et al . - pubmed
The data presented demonstrate that the Ep - CAM molecules are functionally active in the epithelial and carcinoma cells tested , are capable of mediating Ca(++)-independent intercellular adhesions , and are not likely to be involved in cell - substrate adhesion . - pubmed
We show that forskolin and 8 - bromo - cAMP block the basic Fibroblast Growth Factor - induced DNA synthesis , do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin ( E and D1 ) expression without modification of cyclin A level . - pubmed
These results suggest that cAMP may exert its antiproliferative effects through the regulation of cyclin synthesis and cyclin - dependent kinase activation . - pubmed
The expression of the ATPase gene ( PMA1 ) is regulated by glucose ( Rao , R. , Drummond - Barbosa , D. , and Slayman , C. W. ( 1993 ) Yeast 9 , 1075 - 1084 ) and by the TUF / RAP1 / GRF1 transcription factor ( Capieaux , E. , Vignais , M.-L. , Sentenac , A. , and Goffeau , A. - pubmed
Expression of APA1 gene is regulated by the carbon source and requires the protein GCR1 . - pubmed
Deletion of APA1 causes a defective regulation of the PMA1 expression by glucose but has not noticeable effect on the expression of other TUF - regulated genes . - pubmed
Nevertheless the expression of glucose - repressible HXT3 and SNF3 genes is significantly reduced . - pubmed
These results suggest a model in which APA1 acts on a glucose - signaling pathway that controls the expression of several genes that are transcriptionally regulated by glucose . - pubmed
The glutamine synthetase ( GS ) activity of both cell lines was elevated ( up to x 9 ) as glutamine was depleted from normal media ( GMEM + gmine ) . - pubmed
However , there was no apparent significant difference between the two cell lines in the pattern of changes of GS activity in response to glutamine . - pubmed
The discrepancy between this value and the molecular mass of 15,456 + /- 1.5 Da for the copper - free protein determined by electrospray mass spectrometry can be explained by a post - translational processing of the gene product . - pubmed
Subsequently , the precursor is cleaved by a signal peptidase II - like protease and then acetylated at its NH2 - terminal alpha - amino group . - pubmed
A comparison of the primary structure of halocyanin with a number of other blue copper proteins places it into the plastocyanin - related group . - pubmed
After the heart rate was doubled from 60 to 120 beats / min , lactate efflux increased from 0.23 + /- 0.10 ( SE ) to 0.45 + /- 0.12 mumol.min-1.g-1 dry weight with a mean response time of 21.3 s but without an overshoot . - pubmed
DNA sequencing of the V beta RT - PCR products reveals a heterogeneous population of VDJ recombinations and deduced CDR3 amino acid sequences . - pubmed
Plasma LH levels were not altered by the injection of amphetamine . - pubmed
Gas chromatography ( GC ) of free amines is generally unsatisfactory owing to the adsorption and decomposition of the solutes on the column . - pubmed
Derivatization of amines is employed to reduce the polarity and to improve the GC properties . - pubmed
Derivatization reactions for the determination of amines by GC are reviewed with respect to reactivity , selectivity and sensitivity . - pubmed
Blocking of glioma proliferation in vitro and in vivo and potentiating the effects of BCNU and cisplatin : UCN-01 , a selective protein kinase C inhibitor . - pubmed
Seven - hydroxystaurosporine ( UCN-01 ) is a derivative of the nonselective protein kinase inhibitor staurosporine that exhibits significant selectivity for protein kinase C ( PKC ) in comparison to a variety of other intracellular kinases and appears to be well tolerated in vivo at concentrations sufficient to achieve effective inhibition of PKC . - pubmed
The base substitution observed in the BstNI site of Bio - Burs and Bursine-2 was responsible for changing the amino acid at position 222 to serine . - pubmed
The amino acid at this position has been reported to influence a neutralizing epitope on VP2 . - pubmed
Upregulated protein spots were cut from multiple 2 - D gels collected and run on a modified funnel gel to concentrate the proteins and remove the sodium dodecyl sulfate before digestion . - pubmed
A digest can be reanalyzed after deuterium exchange to obtain a second , orthogonal data set to increase the confidence level of protein identification . - pubmed
Kinetics of calcium release from manganese peroxidase during thermal inactivation . - pubmed
In this work we determined that four Ca2 + ions are present in the enzyme as isolated but this was reduced to 2 mol / mol upon treatment with Ca2 + -chelating agents or extensive dialysis of dilute enzyme . - pubmed
One of two relatively tightly bound Ca2 + remaining in the enzyme was released during thermal inactivation at pH 7.2 . - pubmed
Inactive enzyme contained one Ca2 + which could be removed in acidic conditions . - pubmed
The weakly associated Ca2 + normally present in the enzyme did not affect enzyme activity and did not seem to protect the enzyme from thermal inactivation at submicromolar enzyme concentrations . - pubmed
Excess Ca2 + or Mn2 + decreased the rate of the thermal inactivation and Mn2 + stabilized the enzyme more efficiently than Ca2 + at higher temperature . - pubmed
Enzyme stabilization by Mn2 + was proposed to be due to binding of Mn2 + to the Mn2 + substrate binding site . - pubmed
Moreover , Ca2 + was a poor inhibitor of manganese peroxidase activity at pH 4.5 . - pubmed
The present studies examined the distribution and density of the mu and delta receptors in specific brain regions that may mediate the rewarding and reinforcing effects of ethanol , using quantitative autoradiography and the specific mu agonist FK 33 - 824 and delta agonist DPDPE , in their iodinated form . - pubmed
GTP cyclohydrolase I ( GTPCH ) is the rate - limiting enzyme in the formation of tetrahydrobiopterin , the cofactor for catecholamine , indolamine and nitric oxide biosynthesis . - pubmed
DESIGN : The nocturnal ( 2230 h-0200 h ) serum TSH surge , the TSH response to TRH ( 200 microgram as an i.v . - pubmed
Serum TSH levels were measured in samples taken just before ( time 0 ) and 30 minutes , after TRH administration . - pubmed
A role for NO as a mediator of N - formyl - methionyl - leucyl - phenylalanine ( fMLP)-induced chemotaxis was supported by the finding that the NO synthase ( NOS ) inhibitor L - NMMA ( 500 microM ) inhibited chemotaxis ; EC50 for fMLP 28.76 + /- 5.62 and 41.13 + /- 4.77 pmol/10(6 ) cells with and without L - NMMA , respectively . - pubmed
A role for cyclic GMP as a mediator of chemotaxis was supported by the finding that the guanylyl cyclase inhibitor LY 83583 ( 100 microM ) completely inhibited chemotaxis and suppressed the maximal response ; EC50 for fMLP 32.53 + /- 11.18 and 85.21 + /- 15.14 pmol/10(6 ) cells with and without LY 83583 , respectively . - pubmed
The same pattern of inhibition was observed with the G - kinase inhibitor KT 5823 ( 10 microM ) ; EC50 for fMLP 32.16 + /- 11.35 and & gt ; 135 pmol/10(6 ) cells with and without KT 5823 , respectively . - pubmed
The phosphatase inhibitor , 2,3 - diphosphoglyceric acid ( DPG ) ( 100 microM ) which inhibits phospholipase D , attenuated fMLP - induced chemotaxis ; EC50 for fMLP 19.15 + /- 4.36 and 61.52 + /- 16.2 pmol/10(6 ) cells with and without DPG , respectively . - pubmed
Although the NOS inhibitors L - NMMA and L - canavanine ( 500 microM ) failed to inhibit fMLP - induced SAG , carboxy - PTIO caused significant inhibition ( EC50 for fMLP 36.15 + /- 7.43 and 86.31 + /- 14.06 nM and reduced the maximal response from 22.14 + /- 1.5 to 9.8 + /- 1.6 nmol O2-/10(6 ) cells/10 min with and without carboxy - PTIO , respectively ) . - pubmed
This suggests NO is a mediator of fMLP - induced SAG . - pubmed
A role for cyclic GMP as a mediator of SAG was supported by the effects of G - kinase inhibitors KT 5823 ( 10 microM ) and Rp-8 - pCPT - cGMPS ( 100 microM ) which inhibited SAG giving EC50 for fMLP of 36.26 + /- 8.77 and 200.01 + /- 43.26 nM with and without KT 5823 , and 28.35 + /- 10.8 and 49.25 + /- 16.79 nM with and without Rp-8 - pCTP - cGMPS . - pubmed
The phosphatase inhibitor DPG ( 500 microM ) inhibited SAG ; EC50 for fMLP 33.93 + /- 4.23 and 61.12 + /- 14.43 nM with and without DPG , respectively . - pubmed
In conclusion , chemotaxis and SAG induced by fMLP can be attenuated by inhibitors of phospholipase D , NO and cyclic GMP , suggesting a role for these agents in neutrophil activation . - pubmed
The HDL cholesterol concentrations and the ratios of HDL cholesterol to total cholesterol were significantly decreased in the subjects with chronic infection ( 1.24 versus 1.36 mmol / L , P = .026 ; and 0.19 versus 0.22 , P = .018 , respectively ) . - pubmed
In animals , NSAIDs inhibit the cyclooxygenase ( COX ) activity of prostaglandin endoperoxide H synthase , which ultimately blocks the formation of prostaglandins . - pubmed
In this study we show that NSAIDs act as competitive inhibitors of allene oxide synthase ( AOS ) , the cytochrome P450 that initiates plant oxylipin synthesis . - pubmed
We also show that aspirin causes the time - dependent inhibition and acetylation of AOS , which leads the irreversible inactivation of this enzyme . - pubmed
It is possible that aspirin and related NSAIDs could inhibit other P450s that have motifs similar to AOS and consequently serve as potential biochemical targets for this class of drugs . - pubmed
The first database was obtained from eight endurance - trained men dehydrated to four different levels ( 1.1 , 2.3 , 3.4 , and 4.2 % of body wt ) during 120 min of cycling at a power output of 62 - 67 % maximum O2 consumption ( VO2 max ) in the heat [ 33 degrees C and 50 % relative humidity ( RH ) ] . - pubmed
Secretin and other hormones raising intracellular cyclic adenosine monophosphate ( cAMP ) concentrations promote biliary HCO3 secretion by stimulating apical Cl- channels and Cl-/HCO3- exchange ( AE2 ) . - pubmed
Suppression by anti - CD44v6 apparently functioned distinctly to cyclosporine and was most effective in combination with presensitization . - pubmed
Selenium concentrations in blood , milk , casein , and liver and glutathione peroxidase activity in blood and liver are reported as responses per milligram of Se intake . - pubmed
Although Linomide has unacceptable side effects , it seems to have a modest effect on MS disease activity , as measured by MRI . - pubmed
This hepatomegaly can not be explained solely by lipid accumulation and is accompanied by significant increases in hepatocyte proliferative activity ( as evidenced by increased Erk activation , cell - cycle related gene expression , bromodeoxyuridine incorporation , and hepatic DNA content ) with concomitant inhibition of hepatocyte apoptosis ( as evidenced by decreased numbers of apoptotic hepatocytes , induction of several antiapoptotic mechanisms , and decreased activation of procaspase 3 ) . - pubmed
BACKGROUND : One mechanism that directs the action of the second messengers , cAMP and diacylglycerol , is the compartmentalization of protein kinase A ( PKA ) and protein kinase C ( PKC ) . - pubmed
Phenylalanine-24 in the N - terminal region of ammodytoxins is important for both enzymic activity and presynaptic toxicity . - pubmed
To address this problem , a surface - exposed aromatic residue , Phe(24 ) , in the N - terminal region of the most potent Atx , AtxA , was replaced by other aromatic ( tyrosine , tryptophan ) , hydrophobic ( alanine ) and polar uncharged ( serine , asparagine ) residues . - pubmed
Our results suggest a critical involvement of Phe(24 ) in the neurotoxicity of AtxA , apparently at a stage which does not involve the interaction with the known Atx - binding neuronal proteins and catalytic activity . - pubmed
GABA has been reported to stimulate feeding via both ionotropic GABA(A ) and metabotropic GABA ( B ) receptors . - pubmed
Within the heterodimer , the GABA - binding site is localized to GABA ( B ) R1 . - pubmed
In the ventrolateral part of the arcuate nucleus , GABA ( B ) R1 - immunoreactive cell bodies were shown to contain pro - opiomelanocortin and cocaine- and amphetamine - regulated transcript . - pubmed
In the tuberomammillary nucleus , GABA ( B ) R1 - immunoreactive cell bodies expressed histidine decarboxylase , a marker for histamine - containing neurones . - pubmed
The results may provide a morphological basis for the understanding of how GABA regulates the hypothalamic control of food intake and body weight via GABA ( B ) receptors . - pubmed
Gasless VTEM involves a modification of transanal endoscopic microsurgery ( TEM ) that incorporates a standard laparoscopic video camera without a CO ( 2 ) insufflation system . - pubmed
The strain GR4 , which was less competitive than strain S24 , was mutagenized with N - methyl - N '- nitro - N - nitrosoguanidine to obtain pigment - diverse mutants and six selected mutants were screened for symbiotic parameters . - pubmed
Since the drug regimens were different , summed PI for the first acetaminophen dose interval ( SUMPI3.5 - 6 ) and the first naproxen dose interval ( SUMPI3.5 - 9 ) was calculated . - pubmed
In addition , a number of genes were identified which predicted integral membrane proteins with extracellular C - type lectin or immunoglobulin domains and intracellular immunoreceptor tyrosine - based inhibitory motifs ( ITIMs ) and immunoreceptor tyrosine - based activation motifs ( ITAMs ) ( plus their associated signal transduction molecules ) , suggesting that activating and inhibitory receptors have an MHC - independent function and an early evolutionary origin . - pubmed
Furthermore the most potent compounds 2 g and 3 g from each series were screened in vitro for inhibition of both COX-2 and COX-1 catalysed prostaglandin biosynthesis ( radiochemical assay ) . - pubmed
Like most of the commercially available non - steroidal anti - inflammatory drugs ( NSAIDs ) , in the in vitro study , compounds 2 g and 3 g showed anti - inflammatory activity by blocking the metabolism of arachidonic acid to prostaglandin via the cyclooxygenase pathways . - pubmed
Subunit - dependent effects of nickel on NMDA receptor channels . - pubmed
Application of Ni2 + showed that stimulation of NR1a - NR2B receptor channels was dependent on external pH , while voltage - independent inhibition of NR1a - NR2A was less sensitive to pH change . - pubmed
These results indicate that Ni2 + has multiple and complex effects on NMDA channels , which are largely dependent on the NR2 subunit . - pubmed
farciminosum isolates were evaluated for urease activity on Christensen 's urea agar slants and for other qualitative and quantitative urease assays of activity . - pubmed
2.9)% , cholesterol 4.8 ( 0.8)mmol / l , triglycerides 2.7 ( 1.6)mmol / l , HDL 0.83 ( 0.2)mmol / l ; 60 % had albuminuria ( micro 38 % , macro 22 % ) , 47 % were hypertensive , 7 % ( n = 2 ) had retinopathy , 24 % had peripheral neuropathy , none had peripheral vascular disease , 14 % had documented coronary vascular and one participant cerebrovascular disease . - pubmed
superoxide dismutase and catalase activity , mucus and total gastric tissue sulfhydryl group were increased . - pubmed
It is interesting to note that the metal poison , coordinating anions cyanide and azide are also rather weak Cab inhibitors ( Ki in the range of 27 - 55 mM ) , whereas sulfamide is a very weak Cab inhibitor ( Ki of 103 mM ) , although it strongly inhibits Cam ( Ki of 70 microM ) . - pubmed
Surprisingly , phenylboronic and phenylarsonic acids , which have been investigated for the inhibition of all these CAs for the first time , showed very weak activity against the alpha - CA isozymes , but were effective Cab and Cam inhibitors . - pubmed
The best Cab inhibitors were just these two compounds ( Ki 's of 0.20 - 0.33 mM ) , whereas the best Cam inhibitor was sulfamic acid ( Ki of 96 nM ) . - pubmed
These major differences of behavior between the diverse CAs investigated here toward anion inhibitors can be difficultly explained considering the convergent evolution of so diverse enzymes for the binding and turnover of small molecules such as carbon dioxide and anions . - pubmed
RESULTS : Pathologists , by detecting blue dye or carbon particles or alterations in nodal cell populations should attempt to confirm that a node submitted as sentinel is truly sentinel . - pubmed
Ryanodine receptors are found especially within the cerebellum , and alterations in calcium signaling within the cerebellum could impair long - term depression and subsequent motor learning . - pubmed
Biochemical characterization in vitro showed that both ACPs can be loaded with a malonate extender unit by the LnmG acyl transferase ; however , ACP(6 - 2 ) appears to be preferred because the loading efficiency is about 5 - fold that of ACP(6 - 1 ) . - pubmed
These findings provide new insights into the polyketide chain skipping mechanism for modular PKSs . - pubmed
EPR spectroscopy of Cu ( I )- NO adsorption complexes formed over Cu - ZSM-5 and Cu - MCM-22 zeolites . - pubmed
The Cu ( I )- NO adsorption complexes were formed over copper exchanged and autoreduced high siliceous Cu - ZSM-5 and Cu - MCM-22 zeolites and studied by EPR spectroscopy at X- , Q- , and W - band frequencies . - pubmed
The EPR signal intensity of the Cu ( I )- NO species was studied as a function of the NO loading , and the formation of diamagnetic Cu ( I )-( NO )( 2 ) species with rising NO pressure at the expense of paramagnetic Cu ( I )- NO monomers could be demonstrated for both systems at low temperatures . - pubmed
In the present study , we attempted to find out whether metabolic and physiologic characteristics of fibres are important in determining skeletal muscle response to clenbuterol ( an adrenergic receptor agonist ) therapy , as proposed in the treatment of wasting disorders . - pubmed
A significant decline in the succinic dehydrogenase activity was observed , thereby suggesting abnormality in oxidative activity of skeletal muscles in response to clenbuterol therapy . - pubmed
The highest amylase production were attained with lactose , maltose , xylose and starch as carbon sources . - pubmed
Other carbohydrates supported growth but did not induce amylase synthesis and glucose repressed it , indicating catabolite repression in this microorganism . - pubmed
Of the nitrogen sources tested , casaminoacids , ammonium nitrate and sodium nitrate stimulated the highest yield of amylase . - pubmed
No study has evaluated the association of NHL with postload plasma glucose ( PLG ) levels , which are positively associated with BMI . - pubmed
The study compared the net decline of intramyocellular lipids ( IMCL ) during exercise ( n = 18 ) measured by biochemical assay ( BIO ) and Oil Red O ( ORO ) staining on biopsy samples from vastus lateralis muscle and by ( 1 ) H - MR spectroscopy ( MRS ) sampled in an 11 x 11 x 18 - mm(3 ) voxel in the same muscle . - pubmed
Group I , control , was injected intraperitoneally ( ip ) with 1 ml of saline solution ; group II , received DL50 of PQ , ip ; groups III and IV , DL50 of PQ , and simultaneously the first dose of MLT ( 15 mg / kg , ip ) or STS ( 1,5 g / kg , i.p . ) - pubmed
Thirty minutes later , groups III and IV received a second similar dose of MLT and TSS . - pubmed
METHODS : Ala ( GTT ) or Val ( GCT ) polymorphism in the signal peptide of Mn - SOD gene was evaluated using a primer pair to amplify a 107 - bp fragment followed by digestion with restriction enzyme NgoM IV . - pubmed
Intrathecal administration of NAGly ( 700 nmol ) and the pan - cannabinoid receptor agonist HU-210 ( 30 nmol ) reduced the mechanical allodynia induced by partial ligation of the sciatic nerve . - pubmed
The NAGly induced anti - allodynia was dose dependent and , unlike HU-210 , was unaffected by the cannabinoid CB(1 ) and CB ( 2 ) receptor antagonists , AM251 and SR144528 ( 30 nmol ) . - pubmed
NTS1 monomers activate G alpha q beta ( 1 ) gamma ( 2 ) , whereas receptor dimers catalyze nucleotide exchange with lower affinity . - pubmed
CONTEXT : Evidence suggests that testosterone ( T ) influences insulin sensitivity in men . - pubmed
MAIN OUTCOME MEASURES : Each evaluation comprised a 75 - g oral glucose tolerance test with assessment of insulin sensitivity ( fasting insulin levels , homeostatic model assessment for insulin resistance , and Matsuda insulin sensitivity index ) and insulin secretion ( corrected insulin response ) . - pubmed
Serum cortisol , leptin , adiponectin , free fatty acids , IL-6 , C - reactive protein , and TNF - alpha levels were also measured . - pubmed
CONCLUSIONS : 1 ) Acute sex steroid withdrawal reduces insulin sensitivity in young healthy IHH men . - pubmed
2 ) The acuity of the hypogonadism and absence of changes in body mass index or leptin levels suggest that sex steroids modulate insulin sensitivity in the absence of apparent or detectable changes in body composition . - pubmed
WT1 silencing significantly reduced the expression of Nestin and Zyxin and resulted in inhibition of melanoma cell proliferation as determined by a reduced BrdU incorporation . - pubmed
Neurosteroid modulation of GABAA receptors : molecular determinants and significance in health and disease . - pubmed
Identified molecular targets underlying modulation of brain excitability include both the inhibitory GABA(A ) and the excitatory NMDA receptor . - pubmed
A range of molecular mechanisms including the subunit composition of the receptor(s ) , phosphorylation and local steroid metabolism , underpin the region- and neuronal selectivity of action of neurosteroids at synaptic and extrasynaptic GABA ( A ) receptors . - pubmed
GABA - based therapeutic approaches : GABA ( A ) receptor subtype functions . - pubmed
Here , we will review recent progress in identifying the neurosteroid binding site on the GABA ( A ) receptor , and discuss how these discoveries will impact on our understanding of the role of neurosteroids in health and disease . - pubmed
Moreover , when NEC lesions were restricted to the colon , small intestinal tissues that seemed clinically healthy had decreased in vitro glucose absorption due to reduced uptake via the sodium - dependent glucose transporter with little or no involvement of the apical facilitative glucose carrier . - pubmed
Conversely , the hormone gibberellin ( GA ) can antagonise the effects of KNOX overexpression and reduce leaflet number , suggesting that GA may be a negative regulator of leaflet formation . - pubmed
The aim of this paper was to measure the changing desorbable fraction and bioaccessibility of phenanthrene in two different soils with increasing soil - phenanthrene contact time using supercritical fluid extractions ( SFE ) . - pubmed
Desorption profiles were measured every 7d using selective SFE conditions and the results were compared to 14C - phenanthrene mineralisation assays . - pubmed
2H2O ( sodium pentacyanonitrosylferrate ( II ) , nitroprusside , SNP ) and MeN ( H ) OH ( N - methylhydroxylamine , MeHA ) has been studied by means of UV - vis spectroscopy , using complementary solution techniques : FTIR / ATR , EPR , mass spectrometry and isotopic labeling ( 15NO ) , in the pH range 7.1 - 9.3 , I=1 M ( NaCl ) . - pubmed
In the C - terminal reductase domain , the complex structure with the substrate at 2.56 - A resolution unexpectedly showed a ribitylimino intermediate bound at the active site , and hence suggested that the ribosyl reduction occurs through a Schiff base pathway . - pubmed
Lys151 seems to have evolved to ensure specific recognition of the deaminase product rather than the substrate . - pubmed
A detailed comparison reveals that the reductase and the pharmaceutically important enzyme , dihydrofolate reductase involved in the riboflavin and folate biosyntheses , share strong conservation of the core structure , cofactor binding , catalytic mechanism , even the substrate binding architecture . - pubmed
Two stronger EGFR ligands , Spitz and Keren , are expressed by the AMPs themselves and provide an additional , autocrine mitogenic stimulus to the AMPs during late larval stages . - pubmed
Nucleoside diphosphate kinase ( NDPK ) ( EC 2.7.4.6 ) , the enzyme transferring the phosphate residue from ATP to nucleoside diphosphates , is localized mainly in the cytoplasm and mitochondria and in smaller amounts in cell nuclei and the microsomal fraction . - pubmed
Both phytochrome A - activated NDPKs purified to electrophoretic homogeneity have the same molecular mass ( 17 - 18 kDa ) determined by SDS / PAGE . - pubmed
These data suggest that Gpx4 differentially regulates apoptogenic protein release owing to its inner membrane location in mitochondria and its ability to repair cardiolipin peroxidation . - pubmed
Single nucleotide polymorphism ( SNP ) sites for IFN - gamma ( +874 T / A ) , IL-10 ( -1,082 G / A , -819 C / T , -592 C / A ) , TNF - alpha ( -308 G / A ) , and TGF - beta1 ( codon 10 T / C , codon 25 G / C ) genes were observed with the TaqMan allelic discrimination assay . - pubmed
Hourly blood sampling from 8 h before and until 24 h after the birth of the first piglet was used for investigation of temporal changes in plasma concentrations of oxytocin , cortisol , and ACTH . - pubmed
There was a clear temporal development in sow behavior and hormone concentrations ( ACTH , cortisol , and oxytocin ) across parturition ( P & lt ; 0.001 ) , independent of treatment . - pubmed
Floor heating increased the mean concentration of cortisol ( P = 0.02 ; estimated as 29 % greater than in controls ) and tended to increase the mean concentration of ACTH ( P = 0.08 ; estimated as 17 % greater than in controls ) , but we did not find any treatment effect on mean oxytocin concentrations , the course of parturition , or the behavior of sows . - pubmed
In addition , exposure to heat decreased the between - sow variation of plasma oxytocin ( approximately 31 % less relative to control ) and ACTH ( approximately 46 % less relative to control ) . - pubmed
Ventilation in response to exercise was increased in PG ( 49.4+/-6.6L / min ) compared to NP ( 39.8+/-5.4 L / min , at 100W ; p<0.05 ) women , and was further augmented by obesity ( 56.7+/-9.3 L / min , at 100W ; p<0.05 versus PG ) secondary to an elevated metabolic cost of exercise as indicated by no further increase in .V(E)/.V(CO2 ) and .V(E)/.V(O2 ) in PGOB compared to PG women . - pubmed
Docking studies support that 2 binds to the ATP - noncompetitive pocket of glycogen synthesis kinase-3beta ( GSK-3beta ) , which is a putative target of manzamines . - pubmed
It indicates that IL11 , along with its specific receptor , IL11R alpha , and downstream signalling molecules , STAT3 and SOCS3 , are likely to play a role in the progression of endometrial carcinoma . - pubmed
We determined the effects of vitamin D deficiency on bone metabolism : calcium , phosphate and PTH levels . - pubmed
We also analyzed biochemical markers of bone remodeling and bone mineral density ( BMD ) before and 6 months after vitamin D replacement . - pubmed
Angiotensin - converting enzyme 2 ( ACE2 ) catabolizes angiotensin II ( Ang II ) to angiotensin 1 - 7 ( Ang -( 1 - 7 ) ) to limit effects of the renin - angiotensin system . - pubmed
OBJECTIVES : We conducted a prospective randomized trial to compare the clinical impact of conventional risk factor modification to that associated with the addition of coronary artery calcium ( CAC ) scanning . - pubmed
RESULTS : Compared with the no - scan group , the scan group showed a net favorable change in systolic blood pressure ( p = 0.02 ) , low - density lipoprotein cholesterol ( p = 0.04 ) , and waist circumference for those with increased abdominal girth ( p = 0.01 ) , and tendency to weight loss among overweight subjects ( p = 0.07 ) . - pubmed
Within the scan group , increasing baseline CAC score was associated with a dose - response improvement in systolic and diastolic blood pressure ( p & lt ; 0.001 ) , total cholesterol ( p & lt ; 0.001 ) , low - density lipoprotein cholesterol ( p & lt ; 0.001 ) , triglycerides ( p & lt ; 0.001 ) , weight ( p & lt ; 0.001 ) , and Framingham Risk Score ( p = 0.003 ) . - pubmed
TEM analysis and X - ray diffraction ( XRD ) results indicate that the Bi2O3 - core / SnO2 - shell nanowires consist of pure tetragonal alpha - Bi2O3 - phase momocrystalline cores and tetragonal SnO2 - phase polycrystalline shells . - pubmed
Glutamate dehydrogenase ( GDH ) is a mitochondrial enzyme linking the Krebs cycle to the multifunctional amino acid glutamate . - pubmed
Thereby , GDH plays a pivotal role between carbohydrate and protein metabolisms , controlling production and consumption of the messenger molecule glutamate in neuroendocrine cells . - pubmed
Indeed , GDH directly depends on the provision of the co - factor NADH / NAD (+ ) , rendering the enzyme sensitive to the redox status of the cell . - pubmed
Moreover , GDH is allosterically regulated by GTP and ADP . - pubmed
GDH is also regulated by ADP - ribosylation , mediated by a member of the energy - sensor family sirtuins , namely SIRT4 . - pubmed
In the brain , GDH ensures the cycling of the neurotransmitter glutamate between neurons and astrocytes . - pubmed
GDH also controls ammonia metabolism and detoxification , mainly in the liver and kidney . - pubmed
We found that exposure to oxaliplatin induced a significant increase in LC3 lipidation and subsequent LC3 puncta formation . - pubmed
While the proliferation of HCC cells was inhibited upon oxaliplatin exposure , inhibition of autophagy by ATG7 interference and chloroquine pre - treatment further increased the sensitivity to chemotherapy . - pubmed
All samples were characterized by SEM , AFM and thermogravimetric analysis , and the mechanical properties of the PAA grafted sample were determined . - pubmed
At a concentration of 100 muM , 42 natural products inhibited OATP1B1 - mediated [ ( 3 ) H ] E3S uptake by more than 50 % , and five of them significantly inhibited OATP1B1 - mediated [ ( 3 ) H ] E3S by more than 80 % with the following rank order of potency : quercetin & gt ; astragaloside IV & gt ; icariin & gt ; glycyrrhizic acid & gt ; ginsenoside Rc . - pubmed
We performed sevoflurane anaesthesia induction , the end - tidal was kept at 3 and 4 % , during 15 min at each concentration , with no analgesic drug and no neuromuscular blocking agent , and we applied a nociceptive stimulus : tetanus 100 Hz , during 5 s. We set the standard monitorization , BIS , RE , and state entropy ( SE ) along the study . - pubmed
There was a significant difference between RE and SE post - noxious stimulus values at 3 and 4 % end - tidal sevoflurane ( p & lt ; 0.05 ) . - pubmed
The hypothesis that letrozole would arrest growth of the dominant follicle , resulting in emergence of a new follicular wave at a predictable post - treatment interval , was tested . - pubmed
Plasma concentrations of LH and FSH were measured by radioimmunoassay ; plasma concentrations of letrozole were determined by high - performance liquid chromatography tandem mass spectrometry . - pubmed
A single intravenous dose of letrozole did not induce regression of the dominant follicle present at the time of treatment , nor did it directly affect FSH release . - pubmed
Conversely , treatment with letrozole increased endogenous concentrations of LH and extended the lifespan of the dominant follicle , which delayed the next FSH surge and subsequent follicular wave emergence . - pubmed
The proposed biosynthesis of mirubactin comprises the iterative use of a stand - alone carrier - protein - bound substrate , as well as an ester - bond - forming step catalyzed by a C - terminal condensation domain , thus revealing an interesting system for further biochemical studies to gain a deeper understanding of nonribosomal peptide synthetase - catalyzed siderophore biosynthesis . - pubmed
Molecular mechanisms involved in the synergistic interaction of the EZH2 inhibitor 3 - deazaneplanocin A with gemcitabine in pancreatic cancer cells . - pubmed
Pancreatic ductal adenocarcinoma ( PDAC ) is characterized by overexpression of enhancer of Zeste homolog-2 ( EZH2 ) , which plays a pivotal role in cancer stem cell ( CSC ) self - renewal through methylation of histone H3 lysine-27 ( H3K27me3 ) . - pubmed
Against this background , EZH2 was identified as an attractive target , and we investigated the interaction of the EZH2 inhibitor DZNeP with gemcitabine . - pubmed
Moreover , DZNeP enhanced the mRNA and protein expression of the nucleoside transporters hENT1 / hCNT1 , possibly because of the significant reduction of deoxynucleotide content ( e.g. - pubmed
Furthermore , DZNeP and DZNeP / gemcitabine combination significantly reduced the volume of PDAC spheroids growing in CSC - selective medium and decreased the proportion of CD133 + cells . - pubmed
The role of fluorine in stabilizing the bioactive conformation of dihydroorotate dehydrogenase inhibitors . - pubmed
Dihydroorotate dehydrogenase ( DHODH ) is an important drug target due to its prominent role in pyrimidine biosynthesis . - pubmed
Leflunomide and brequinar are two well - known DHODH inhibitors , which bind to the enzyme in the same pocket with different binding modes . - pubmed
We have recently realized a series of new inhibitors based on the 4 - hydroxy-1,2,5 - oxadiazole ring , whose activity profile was found to be closely dependent on the degree of fluorine substitution at the phenyl ring adjacent to the oxadiazole moiety ; a positive influence of fluorine on the DHODH inhibitory potency was observed previously [ Baumgartner et al . - pubmed
For pilin antigenic variation ( Av ) to occur , a guanine quartet ( G4 ) structure must form upstream of pilE. - pubmed
Different studies4 , 5 relate that stress increases in children and adolescents with overweight and obesity , and consequently their salivary physiological responses ( AEA salivary alpha - amylase , cortisol , cytokines , leptin ) , so in this study we relate these two parameters to see their progress through a program of physical activity . - pubmed
These results show that PVA - SNO films might emerge as a new material with potential for the topical treatment of microvascular skin disorders . - pubmed
In vivo efficacy of HD0471953 : a novel GPR119 agonist for the treatment of type 2 diabetes mellitus . - pubmed
We examined the relationship between childhood measures of PAH exposure and prevalence of attention deficit / hyperactivity disorder ( ADHD ) , learning disability ( LD ) , and special education ( SE ) in a nationally representative sample of 1,257 U.S. children 6 - 15 years of age . - pubmed
Multivariate logistic regression accounting for survey sampling was used to determine the associations between PAH metabolites and ADHD , LD , and SE . - pubmed
Finally , for a more radical approach towards a safe MEK inhibitor , we validate the potential of multi - kinase inhibitor curcumin , especially the nano - curcumin made out of pure curcumin with greater bioavailability ; in repealing tumour - shed TGF - beta - induced Treg cell augmentation . - pubmed
Decrease in ubiquitin - HIF-1alpha was also shown , and leads to higher HIF-1alpha protein levels in DZN group . - pubmed
We showed that LOX3 activity and its ability to produce 9 - hydroperoxyoctadecadienoic acid ( 9 - HPOD ) from linoleic acid were significantly lower in transgenic seeds than in wild - type seeds by measuring the ultraviolet absorption of 9 - HPOD at 234 nm and by high - performance liquid chromatography . - pubmed
In a post - hoc analysis , non - depressed non - obese women were found to have significantly higher cortisol levels compared to women with depression , obesity or both ( p & lt ; 0.05 ) . - pubmed
The major cellular fatty acids were C18 : 1omega7c / C18 : 1omega6c , C16 : 0 and C16 : 1omega7c / C16 : 1omega6c . - pubmed
H2S is synthesized from L - cysteine and/or L - homocysteine by cystathionine beta - synthase , cystathionine gamma - lyase and cysteine aminotransferase together with 3 - mercaptopyruvate sulfurtransferase . - pubmed
H2S is enzymatically oxidized in mitochondria to thiosulfate and sulfate by specific enzymes , sulfide : quinone oxidoreductase , persulfide dioxygenase , rhodanese and sulfite oxidase . - pubmed
To explore the role of the canonical BMP4 / SMAD signaling pathway in the pathogenesis of chordoma , we investigated , in 40 skull base chordomas , the expression of three major components of the signaling axis : BMP4 , phospho - SMAD5 and SMAD4 . - pubmed
BACKGROUND : Acute kidney injury due to glomerular bleeding has been described with IgA nephropathy and supratherapeutic warfarin anticoagulation . - pubmed
When exposed to low intensities of light that modify the redox state of the plastoquinone pool , the so - called state transitions redistribute energy between photosystem I and II . - pubmed
The phospholipid cardiolipin is indispensable for eukaryotes to activate mitochondria , and it was previously reported that two phylogenetically distinct types of enzyme synthesizing cardiolipin , one with two phospholipase D domains ( CLS_pld ) and the other with a CDP - alcohol phosphatidyltransferase domain ( CLS _ cap ) , are patchily and complementarily distributed at higher taxonomic ( e.g. - pubmed
albolabris venom elicited strong antibody responses , and from their egg yolk IgY was isolated and purified , which exhibited a single protein band on SDS - PAGE and two bands ( about 65 and 35 kDa , respectively ) under reduced conditions . - pubmed
A total of 11 transformation products ( TP ) ( mainly phase - I ) were quantified by liquid chromatography - tandem mass spectrometry ( LC - MS / MS ) and their total amount corresponded to 33 % of the CBZ taken up . - pubmed
Further 10 TPs ( phase - I and II ) were identified by LC - high resolution mass spectrometry screening , likely comprising another 12 % of CBZ . - pubmed
Effect of Conjugated Linoleic Acid Supplementation on Serum Leptin Concentration : A Systematic Review and Meta - Analysis . - pubmed
BACKGROUND : There are controversies regarding the effect of conjugated linoleic acid ( CLA ) on serum leptin . - pubmed
METHODS : Eleven 2 - piece ZrO2 implants , each mounted with ZrO2 abutments plus bonded monolithic lithium disilicate ( LS2 ) restorations , were grouped for 3.3 mm ( A ) and 4.1 mm ( B ) diameter samples . - pubmed
CONCLUSIONS : The examined 2 - piece ZrO2 implant system mounted to LS2 - restorations seems to be a stable unit under in - vitro conditions with mechanical properties compared to loading capacity of physiological force . - pubmed
A method for the quantitative determination of dibutyl phthalate ( DBP ) , benzyl butyl phthalate ( BBP ) , bis ( 2 - ethylhexyl ) adipate ( DEHA ) , bis ( 2 - ethylhexyl ) phthalate ( DEHP ) , di - n - octyl phthalate ( DNOP ) , dioctyl terephthalate ( DOTP ) , diisononyl phthalate ( DINP ) , and diisodecyl phthalate ( DIDP ) in medical infusion sets was developed and validated using gas chromatography coupled with triple quadrupole mass spectrometry ( GC - MS / MS ) in the multiple reaction monitoring ( MRM ) mode . - pubmed
Chemically , strengthening the previous hypothesis , the collected particles were mainly carbonaceous ( 68%-93 % ) with the maximum budget of the polyaromatic hydrocarbons being recorded near the surface ( 1st/5th floor : 1.84 , 1.07 , 1.15 for PM10 , PM2.5 and PM1 , respectively ) . - pubmed
With respect to the health hazards , the calculation of the Benzo [ a ] Pyrene toxicity equivalency factors underlined the importance of the height of residence in buildings for the level of the exposure ( 1st/5th floor : B[a]PTEQ : 1.82 , 1.12 , 1.10 , B[a]PMEQ : 1.85 , 1.13 , 1.09 for PM10 , PM2.5 and PM1 , respectively ) . - pubmed
Twenty - seven unique UAA alleles identified from the MHC I genes of these breeds were analyzed concerning amino acid composition , homology , and phylogenetic relationships . - pubmed
By systematic mutational analysis , we define conserved dual bi - arginine ( RXR ) motifs that are required for NGF- and phosphoinositide - regulated DOR export from intracellular compartments in neuroendocrine cells . - pubmed
Sodium - glucose co - transporter 2 ( SGLT2 ) inhibitors inhibit glucose re - absorption in the proximal renal tubules . - pubmed
The search of the literature revealed many possible cardioprotective mechanisms , because SGLT2 inhibitors ( i ) increase natriuresis and act as diuretics with unique properties leading to a reduction in preload and myocardial stretch ( the diuretic hypothesis ) ; ( ii ) decrease blood pressure and afterload ( the blood pressure lowering hypothesis ) , ( iii ) favor the production of ketones , which can act as a ' superfuel ' in the cardiac and renal tissue ( the ' thrifty substrate ' hypothesis ) , ( iv ) improve many metabolic variables ( the metabolic effects hypothesis ) , ( v ) exert many anti - inflammatory effects ( the anti - inflammatory effects hypothesis ) , ( vi ) can act through the angiotensin II type II receptors in the context of simultaneous renin - angiotensin - aldosterone - system ( RAAS ) blockade leading to vasodilation and positive inotropic effects ( the RAAS hypothesis ) , ( vii ) directly decrease the activity of the upregulated in heart failure Na +- H + exchanger in myocardial cells leading to restoration of mitochondrial calcium handling in cardiomyocytes ( the sodium hypothesis ) . - pubmed
PATIENTS AND METHODS : : In this prospective comparative study , 32 eyes with silicone oil tamponade of high viscosity ( 5700 CS ) and 32 normal fellow eyes were included . - pubmed
CONCLUSIONS : : It seems that GAT overestimates IOP in eyes with high - viscosity silicone oil compared with NT , while both IOP measurement techniques in normal eyes provide similar values . - pubmed
AChE activity in the cerebral cortex was lower in the infected group than in the control ; there was an increase in the activity of NTPDase , 5 '- nucleotidase and ADA , possibly indicating greater hydrolysis of ATP ( P & lt ; 0.001 ) , ADP ( P & lt ; 0.01 ) and AMP ( P & lt ; 0.01 ) , followed by increased adenosine deamination ( P & lt ; 0.001 ) . - pubmed
In this project , an N - terminal labeling mass spectrometry method , terminal amine isotopic labeling of substrates ( TAILS ) , was optimized for the in - depth analysis of PHG and validated using a test protease ( trypsin ) with known cleavage specificity . - pubmed
, aprepitant ) are known to exert , via the NK-1R , an antitumor action ; TNBC cells die by apoptosis . - pubmed
At last , we detected stimulated by retinoic acid gene 6 homolog ( STRA6 ) , a cell surface receptor formerly known as a gene preventing podocytes from over - proliferative lesion induced by HIV infection and was up - regulated by retinoic acid , expressed at a much higher level in SRNS kidneys . - pubmed
Unfortunately , none of the biomarkers assessed were indicative of passing training , however , glucose , blood urea nitrogen , and creatine kinase ( CK ) levels stood out as abnormal . - pubmed
Lipids in milk are secreted as a triacylglycerol core surrounded by a trilayer membrane , the milk fat globule membrane ( MFGM ) . - pubmed
We evaluated fasting blood - glucose ( FBG ) , urinary albumin / creatinine ratio ( UACR ) , body weight , and kidney index ( KI ) in the different groups . - pubmed
Initially , the use of Adam - Gibbs approach revealed that RIV 's molecular mobility ( below its glass transition temperature ) is significantly reduced in the presence of SOL , while the use of ATR - FTIR spectroscopy showed the formation of hydrogen bonds ( HBs ) between the two ASD components , indicating that these two mechanisms can be considered as responsible for system 's physical stability . - pubmed
radiata , and found that gibberellic acid ( GA ) significantly inhibited , whereas paclobutrazol ( PBZ ) , abscisic acid ( ABA ) , and ethrel promoted bulblet development , especially PBZ , a GA biosynthesis inhibitor . - pubmed
Furthermore , GA reduced endogenous cytokinin ( CK ) content , as well as the activities of carbohydrate metabolism enzymes , including sucrose synthase ( SUS ) and glucose-1 - phosphate adenylyltransferase ( AGPase ) , by downregulating the expression levels of LrSUS1 , LrSUS2 , and genes encoding AGPase large and small subunits . - pubmed
PBZ had the opposite effect to GA on carbohydrate metabolism ; it decreased endogenous GA15 and GA24 , thereby promoting bulblet development . - pubmed
ABA promoted endogenous auxin content and the activities of starch synthesis enzymes , especially soluble starch synthase ( SSS ) and granule - bound SS ( GBSS ) , through the up - regulation of the expression levels of LrSS1 , LrSS2 , and LrGBSS1 genes , which could also result in the accumulation of carbohydrates in the bulblets and promote their development . - pubmed
In addition , ethrel application partly promoted bulblet development by promoting endogenous CK content . - pubmed
Treatment of the enzyme with sodium dodecyl sulfate followed by polyacrylamide gel electrophoresis gave a single band with a relative mobility suggesting a molecular weight of 76,000 plus and minus 2000 . - pubmed
Sulfhydryl group modification of thymidylate synthetase and its effect on activity and ternary complex formation . - pubmed
Saturated and monoenoic iso ( C15 - C19 ) and anteiso ( C15 , C17 ) fatty acids and saturated normal ( C14 - C18 ) and monoenoic normal ( C16 , C18 ) fatty acids were shown to be shown to be present by capillary gas chromatography - mass spectrometry . - pubmed
[ Effect of 4 - hydroxypyrazolo [ 3,4 - d ] pyrimidine on uricase activity ( author 's transl ) ] . - pubmed
We investigated the ultrastructural localization of annexin V a Ca ( 2 +)-dependent phospholipid- and membrane - binding protein in the nervous system , heart , and skeletal muscles . - pubmed
The observation that plasma membranes in cells expressing annexin V have the protein associated with them is in agreement with previous data on Ca ( 2 +)-dependent binding of the protein to brain and heart membranes , and on existence of both EGTA- and Triton X-100 - extractable and resistant fractions of annexin V in these membranes . - pubmed
An increase in CNS dopamine neurotransmission , resulting from a competitive blockade of high - affinity dopamine uptake mediated by both D1 and D2 dopamine receptors , is a primary determinant of the behavioral effects of cocaine . - pubmed
Anantin -- a peptide antagonist of the atrial natriuretic factor ( ANF ) . - pubmed
Protein chemical data and FAB - MS have identified anantin to be a cyclic polypeptide consisting of 17 common L - amino acids . - pubmed
The apparent differences between the glutamate - induced current and nerve - evoked synaptic response revealed by TI-233 can be explained by open - channel block of the glutamate - activated ion - channel , and do not confute the hypothesis that glutamate is the natural transmitter substance at this junction . - pubmed
However , it is likely that Ca channel in smooth muscle is not controlled by cyclic AMP , in contrast to the cardiac muscle . - pubmed
There are also different ( voltage- and Ca - activated ) types of K channel . - pubmed
Endotoxaemia increased the rates of lactate formation , at all levels of insulin , both at 24 h ( approximately 32 % ) and 48 h ( approximately 26 % ) . - pubmed
There are two methionyl tRNA species in the cytoplasmic and mitochondrial fractions , one of which is unique to each fraction , indicating that the KtRNA fraction is free of CtRNA contamination . - pubmed
KtRNA isolated by DEAE chromatography or agarose gel electrophoresis contains additional small RNAs which hybridize with both minicircle and maxicircle DNA . - pubmed
The anti - CD2 stimulation strongly potentiates the PGE2 - induced cAMP production while both PMA and A23187 produced a less potent effect . - pubmed
On the opposite the anti - CD2 treatment is without any effect on the basal cAMP level contrasting with a marked increase of intracellular cAMP concentrations with A23187 or the combination of A23187 and PMA . - pubmed
Phosphorylation of chicken gizzard myosin : myosin filament hypothesis of calcium regulation . - pubmed
ii ) ATP also induced disassembling of gizzard myosin filaments . - pubmed
iii ) Enzymic phosphorylation of gizzard myosin light chains rendered both the myosin conformation and the myosin filaments resistant to the actions of ATP . - pubmed
iv ) Very high concentrations of magnesium were required for formation of the ATP - resistant filaments as well as for superprecipitation ( a model contraction ) of actomyosin suspensions . - pubmed
v ) ITP was a very poor substrate for MLCK , and was accordingly incapable of inducing " Ca - tension " in glycerinated fibers of gizzard muscle , but it did induce " Mg - tension . " - pubmed
Primarily from these findings , it was proposed that the mechanism of gizzard muscle contraction involves ATP - induced changes in the morphology of myosin filaments which are reversibly altered by enzymic phosphorylation and dephosphorylation of myosin light chains in the presence of relatively high concentrations of magnesium . - pubmed
CR1 belongs to a group of proteins that contain a structural element characterized by its size of 60 - 65 amino acids , and four conservatively positioned cysteines , which engage in a self - contained 1 - 3 , 2 - 4 disulphide arrangement . - pubmed
This paper reports further optimization of the PEI bonding chemistry in the preparation of HP - IEC columns for oligonucleotides and tRNA species . - pubmed
Synthesis of chicken calcitonin - gene - related peptide ( cCGRP ) by application of sulfoxide - directed disulfide - bond - forming reaction . - pubmed
Hepatitis B surface antigen ( HBsAg ) was detectable up to 3 weeks , while hepatitis B e antigen ( HBeAg ) persisted only for 3 days in mosquitoes after feeding on hepatitis B viral antigens - positive blood . - pubmed
The racemase has one pyridoxal phosphate molecule per 42 - kDa subunit , has a Vmax of 3570 units / mg and a Km of 7.8 mM in the L to D direction , and has a Vmax of 1210 units / mg and a Km of 2.2 mM in the D to L direction . - pubmed
The purified enzyme is inhibited in a time - dependent manner by both L - and D -( l - aminoethyl ) phosphonates ( Ala - P ) , confirming observations of Atherton et al . - pubmed
Thus , enzyme after exposure to [ 14C ] Ala - P or [ alpha-3H ] Ala - P and gel filtration contains stoichiometric amounts of radioactive label , but denaturation quantitatively releases intact Ala - P into solution as revealed by high - performance liquid chromatography and cocrystallization with authentic material . - pubmed
The Ala - P isomers are slow binding inhibitors of this racemase as is the alpha , alpha '- dimethyl analogue but not the D or L isomers of the corresponding phosphinate.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
Properties of SV 40 rescued from actinomycin D - sensitive and actinomycin D - resistant transformed hamster cells . - pubmed
The potential effect of maternal caffeine ingestion upon total brain protein and the concentration of two prototype neuropeptides , thyrotropin - releasing hormone ( TRH ) and its derivative , cyclo ( His - Pro ) in neonates was examined during the nursing period in the context of variable maternal protein intake . - pubmed
By contrast , no significant alterations were observed in TRH concentrations between caffeine and control groups . - pubmed
The enzyme - inhibitor complex was not dissociated by 4 M KCl . - pubmed
To determine if this effect was due to Ca2 + buffering , a mutant form of calmodulin that has an eight - amino acid deletion in the central helix ( CaM-8 ) was used . - pubmed
The mutated calmodulin binds Ca2 + normally , but alters the ability to interact with known Ca2 +/calmodulin - activated enzymes . - pubmed
In vitro competition analysis using HIT cell extracts verified that in the presence of Ca2 + , CaM-8 exhibited at least a 100 - fold lower affinity for calmodulin - binding proteins than did normal CaM in this model beta - cell . - pubmed
This condition was progressive and characterized by elevated blood glucose that coincided with reduced levels of pancreatic insulin and low circulating serum insulin levels . - pubmed
Carbachol , which is thought to act in beta - cells through the release of intracellular Ca2 + stores and activation of protein kinase - C , restored both phases of secretion to normal levels . - pubmed
These results suggest that disruption of intracellular Ca2 + homeostasis alone is sufficient to interfere with the insulin secretion pathway . - pubmed
Dose linearity of the pharmacokinetics of the new H +/ K (+)- ATPase inhibitor pantoprazole after single intravenous administration . - pubmed
Pantoprazole is a specific inhibitor of the H +/ K (+)- ATPase of the gastric parietal cell . - pubmed
The dose - independent elimination of pantoprazole was attributed to the lack of interaction of the drug with cytochrome P450 . - pubmed
[ Clinical significance of thyrotropin measurement by immunoradiometric assay and thyrotropin - releasing hormone test ] . - pubmed
The GABAB receptor antagonist p -( 3 - aminopropyl )- p - diethoxymethyl - phosphinic acid ( CGP 35348 ) abolished the inhibitory effect of an increase in D - glucose by 60 and 100 mM on [ 3H ] NE release but only tended to diminish the inhibition caused by an increase by 320 mM. - pubmed
Arc - L1 - Arc protects operator DNA in a manner indistinguishable from that of wild - type Arc in DNase I and copper - phenanthroline footprinting studies , but the covalent attachment of the two monomers results in enhanced affinity for operator DNA . - pubmed
INTERVENTION : Stepped - care therapy ( pravastatin , nicotinic acid , cholestyramine , and gemfibrozil ) to decrease total cholesterol levels to less than 4.1 mmol / L ( 160 mg / dL ) and the ratio of LDL cholesterol to HDL cholesterol to less than 2.0 . - pubmed
RESULTS : Mean lipid levels at baseline were as follows : total cholesterol , 5.5 mmol / L ( 214 mg / dL ) ; LDL cholesterol , 3.6 mmol / L ( 140 mg / dL ) ; HDL cholesterol , 1.1 mmol / L ( 42 mg / dL ) ; and triglycerides , 1.8 mmol / L ( 159 mg / dL ) . - pubmed
With pravastatin , changes in levels from baseline were -22 % for total cholesterol , -32 % for LDL cholesterol +8 % for HDL cholesterol , and -15 % for triglycerides ( P & lt ; 0.001 for all comparisons ) . - pubmed
-11 % for LDL cholesterol , +8 % for HDL cholesterol , and -10 % for triglycerides ( P & lt ; 0.001 for all comparisons ) . - pubmed
With 2.25 to 3 g of nicotinic acid , these changes were -7 % for total cholesterol ( P = 0.007 ) , -14 % for LDL cholesterol ( P & lt ; 0.001 ) , +6 % for HDL cholesterol ( P = 0.02 ) , and -13 % for triglycerides ( P = 0.03 ) . - pubmed
With cholestyramine , total cholesterol and LDL cholesterol levels were unchanged compared with the previous step ; the change in HDL cholesterol level was -8 % ( P = 0.03 ) ; and the change in triglyceride level was +46 % ( P & lt ; 0.001 ) . - pubmed
With gemfibrozil , total cholesterol level was unchanged ; the additional change in LDL cholesterol level was +12 % ( P = 0.09 ) ; the change in HDL cholesterol level was +12 % ( P = 0.03 ) ; and the change in triglyceride level was -37 % ( P & lt ; 0.001 ) . - pubmed
Pravastatin with nicotinic acid and pravastatin with gemfibrozil are well - tolerated combinations that can maintain target LDL cholesterol levels , decrease triglyceride levels , and increase HDL cholesterol levels . - pubmed
In case 3 , the plasma adrenocorticotropin level was unchanged during treatment in the first week , despite marked reductions in plasma and 24 - hour urinary free cortisol levels . - pubmed
Inhibition was found to require the coaggregation of the two receptors and to depend on the presence of a tyrosine - based inhibition motif ( ITIM ) in the intracytoplasmic domain of Fc gammaRIIB . - pubmed
We report here that the coaggregation with Fc gammaRIIB does not prevent Fc epsilonRI from triggering activation signals in BMMC and induces the tyrosine phosphorylation of Fc gammaRIIB . - pubmed
At 1 week after MI , circulating plasma levels of adrenaline , angiotensin II , atrial natriuretic factor ( ANF ) and vasopressin were significantly elevated . - pubmed
For example , one molecule that we have previously identified as a component of the IPM , laminin beta2 ( formerly known as s - laminin ) , is implicated in the differentiation of rod photoreceptor cells . - pubmed
Adrenal steroids act through two receptor subtypes , the glucocorticoid receptor ( GR ) and the mineralocorticoid receptor , and activation of each receptor subtype has distinct biochemical and physiological consequences . - pubmed
We have examined adrenal steroid regulation of the neurotrophins brain - derived neurotrophic factor , neurotrophin-3 , and basic fibroblast growth factor , as well as the growth associated protein GAP-43 , through activation of GR or mineralocorticoid receptor with selective agonists . - pubmed
Thus the extent to which adrenal steroids regulate hippocampal neurotrophic and growth - associated factors , appears to be dependent both on the adrenal steroid receptor subtype activated and on the hippocampal subregion examined . - pubmed
Multiple doses of nicotine were tested and 0.5 mg / kg nicotine , given on both training and testing days , improved contextual learning but had no effect on formation of an auditory CS - US association . - pubmed
The nicotinic receptor antagonist mecamylamine ( 1 and 2 mg / kg ) did not alter contextual fear conditioning but mecamylamine did prevent the nicotine - associated increase in contextual learning . - pubmed
A higher dose of nicotine ( 1 mg / kg , training day only ) interfered with contextual conditioning when the context was paired with both the CS and US , but had no effect on the auditory CS - US association . - pubmed
Here we have constructed GFP - fusions of N- and C - domain and found that the N - terminal domain showed CAP - specific enrichment at the anterior and posterior ends of cells like endogenous CAP irrespective of the presence of the proline rich region . - pubmed
Effect of food on the pharmacokinetics of oral MMI270B ( CGS 27023A ) , a novel matrix metalloproteinase inhibitor . - pubmed
MMI270B is a matrix metalloproteinase inhibitor ( MMPI ) with in vitro and in vivo activity . - pubmed
Functional studies demonstrate that cAMP represses whereas tumor necrosis factor - alpha , an activator of NF - kappaB , stimulates promoter activity . - pubmed
Consistently , inhibition by cAMP is reverted by increasing intracellular HATs and is completely abolished by site - specific mutagenesis of the cAMP response element . - pubmed
In contrast , specific mutations in the NF - kappaB response element abolish responsiveness to p65 / RelA and HATs without affecting cAMP - dependent repression . - pubmed
Niemann - Pick type C disease is characterized by the accumulation of cholesterol and other lipids within the lysosomal compartment , a process that is often accompanied by a reduction in acid sphingomyelinase activity . - pubmed
These data suggest that cholesterol - induced inhibition is a posttranslational event , perhaps involving cofactor mediated modulation of enzymatic activity or alterations in acid sphingomyelinase protein trafficking and maturation . - pubmed
This study investigated whether the exaggerated response to carbon dioxide in panic disorder ( PD ) can be modulated by antagonists of muscarinic cholinergic receptors . - pubmed
The KOR gene encodes kappa(1)-sites , which specifically bind arylacetamide compounds , and the possible existence of kappa - opioid receptor subtypes derived from another kappa - opioid - receptor gene , yet to be characterized , remains a very contentious issue . - pubmed
kappa ( 2 )- Opioid receptors are described as binding sites typically labelled by the non - selective benzomorphan ligand [ 3H ] bremazocine in the presence of mu - , delta - and kappa ( 1 )- opioid receptor blocking ligands . - pubmed
Scatchard analysis showed that 68+/-12 % of the binding sites arise from the MOR gene , 27+/-1 % from the DOR gene and 14.5+/-0.2 % from the KOR gene , indicating that the three known genes account for total [ 3H]bremazocine binding . - pubmed
MEASUREMENTS AND MAIN RESULTS : We measured changes in haemodynamic variables , dose of norepinephrine required to maintain a mean arterial pressure greater than 70 mmHg and plasma concentrations of complement anaphylatoxins and several cytokines . - pubmed
Salivary non - immunoglobulin agglutinin inhibits human leukocyte elastase digestion of acidic proline - rich salivary proteins . - pubmed
However , human leukocyte elastase , arriving in gingival exudates from inflamed periodontal tissues , degrades the acidic proline - rich salivary proteins , preventing binding to hydroxylapatite surfaces . - pubmed
Here it is reported that high - molecular - weight non - immunoglobulin salivary agglutinin inhibited the proteolytic action of human leukocyte elastase on purified acidic proline - rich salivary proteins . - pubmed
The addition of antibody against salivary agglutinin blocked the inhibitory effect of parotid saliva on exogenously applied human leukocyte elastase , allowing for the elastase - mediated digestion of the salivary acidic proline - rich salivary proteins . - pubmed
Salivary agglutinin , therefore , is a physiologically important inhibitor of human leukocyte elastase and is able to inhibit elastase - mediated digestion of salivary acidic proline - rich proteins . - pubmed
IgE - mediated anaphylaxis after first intravenous infusion of cyclosporine . - pubmed
Gamma - TQ released mitochondrial cytochrome c to the cytosol , induced the cleavage of poly ( ADP - ribose ) polymerase , and depleted intracellular glutathione . - pubmed
Unlike xenobiotic electrophiles , gamma - TQ is a highly cytotoxic arylating electrophile that stimulates apoptosis in several cancer cell lines including cells that express MDR through both P - glycoprotein and MRP - associated proteins . - pubmed
Serial Dexamethasone Suppression Tests and Clinical Outcome in ECT . - pubmed
The Ca ( 2 +)/ calmodulin - dependent protein phosphatase , calcineurin , modulates a number of key Ca ( 2 + ) signaling pathways in neurons , and has been implicated in Ca ( 2 +)-dependent negative feedback inactivation of N - methyl - D - aspartate receptors and voltage - sensitive Ca ( 2 + ) channels . - pubmed
In contrast , we report here that three mechanistically disparate calcineurin inhibitors , FK-506 , cyclosporin A , and the calcineurin autoinhibitory peptide , inhibited high - voltage - activated Ca ( 2 + ) channel currents by up to 40 % in cultured hippocampal neurons , suggesting that calcineurin acts to enhance Ca ( 2 + ) currents . - pubmed
The immunosuppressant , rapamycin , and the protein phosphatase 1 / 2A inhibitor , okadaic acid , did not decrease Ca ( 2 + ) channel current , showing specificity for effects on calcineurin . - pubmed
FK-506 also inhibited substantially more Ca ( 2 + ) channel current in 4 - week - old vs. 2 - week - old cultures , an effect paralleled by an increase in calcineurin A mRNA levels . - pubmed
Identification of a non - canonical tyrosine - based endocytic motif in an ionotropic receptor . - pubmed
Three amino acids in the C terminus of P2X ( 4 ) ( Tyr(378 ) , Gly(381 ) , and Leu(382 ) ) compose a non - canonical tyrosine - based sorting signal of the form YXXGL . - pubmed
Mutation of either the tyrosine - binding pocket of the mu2 subunit of adaptor protein-2 or the YXXGL motif in the receptor C terminus caused a decrease in receptor internalization and a dramatic increase in the surface expression of P2X ( 4 ) receptors . - pubmed
The YXXGL motif represents a non - canonical tyrosine - based sorting signal that is necessary for efficient endocytosis of the P2X ( 4 ) receptor . - pubmed
Preclinical evaluation of a radiosensitizing effect of gemcitabine in p53 mutant and p53 wild type bladder cancer cells . - pubmed
DNA damage was evaluated by flow cytometric assessment of poly ( ADP - ribose ) polymerase , and apoptosis was determined by terminal - deoxynucleotidyltransferase - mediated dUTP nick - end labeling and flow cytometric assessment after annexin - V and propidium iodide labeling . - pubmed
Gemcitabine alone had a dose - dependent cytotoxic and apoptosis inducing effect on all TCC cell lines independent of p53 status . - pubmed
CONCLUSIONS : Gemcitabine is effective in TCC cell lines independent of p53 status . - pubmed
Both chains were labeled with two different tags : a ( His)(6 ) was introduced at the C - terminal end of alpha chain , while beta chain was terminated by c - myc . - pubmed
Since an isolated alpha chain is unstable unless it is associated with a beta chain , this design permits rapid separation of alpha , beta - heterodimer from unpaired beta chain in a single step of Ni - NTA Agarose chromatography yielding 90 % pure alpha , beta - TCR . - pubmed
Regular drug use ( use at least once a month ) other than cannabis whilst a VDP was found to be highest among males , with regular ecstasy use reported by 4 % and regular cocaine use reported by 2 % . - pubmed
The total cholesterol concentration and CK , AST and LDH activities in the serum showed higher values than those of control calves . - pubmed
Additional specimens were prepared and examined with TEM using a silver nitrate - staining technique . - pubmed
When bonded according to manufacturer 's directions , Panavia F produced bond strengths that were significantly lower than Bistite II DC ( p<0.05 ) . - pubmed
The placement of an additional layer of a LVBR improved significantly the bond strengths of Panavia F ( p<0.05 ) , but not of Bistite II DC ( p>0.05 ) . - pubmed
Phosphorylated amino acids : model compounds for solid - state 31P NMR spectroscopic studies of proteins . - pubmed
The cross - polarization buildup rates and longitudinal relaxation times of 31P and 1H were - determined and compared with the values measured for a triphosphate ( GppCH2p ) bound to a crystalline protein ( Ras ) . - pubmed
Both mGluR1 and mGluR5 mediate Ca2 + release and inward currents in hippocampal CA1 pyramidal neurons . - pubmed
The selective group I mGluR agonist , ( RS )- 3,5 - dihydroxyphenylglycine ( DHPG ) , evoked a transient increase in intracellular Ca2 + levels ( [ Ca2+]i ) , within neuronal somas and apical dendrites , together with a relatively long lasting inward current ( I(DHPG ) ) . - pubmed
The selective mGluR1 and mGluR5 antagonists , ( S )-(+)- alpha - amino - a - methylbenzeneacetic acid ( LY367385 ; 100 microM ) and 2 - methyl-6 -( phenylethynyl )- pyridine ( MPEP;10 microM ) , respectively , markedly inhibited both I(DHPG ) and the DHPG - evoked increase in [ Ca2+]i . - pubmed
Moreover , these antagonists inhibited the Ca2 + response by more than 50 % suggesting a synergistic interaction between mGluR1 and mGluR5 . - pubmed
This study demonstrates that in CA1 pyramidal neurons group I mGluR - mediated inward currents and Ca2 + release from intracellular stores are enhanced under depolarising conditions and that mGluR1 and mGluR5 both contribute to these phenomena . - pubmed
N - SasA adopts a canonical thioredoxin fold but lacks the catalytic cysteine residues . - pubmed
Our analysis suggests that the structural differences between N - SasA and KaiB are the result of only a few critical amino acid substitutions . - pubmed
[ Influences of FVP1 on the curative and negative effects of CTX ] . - pubmed
OBJECTIVE : To observe the influences of FVP1 on both curative and negative effects of CTX . - pubmed
Site - specific vectorial immobilization of YCC via Cys102 on single - walled carbon nanotubes ( SWNT ) thus provides a selective interface between nanoscopic electronic devices and complex enzymes . - pubmed
Cytokine - induced prostaglandin E2 production and cyclooxygenase-2 expression in dental pulp cells : downstream calcium signalling via activation of prostaglandin EP receptor . - pubmed
AIM : To determine whether ( i ) proinflammatory cytokines stimulate prostaglandin E ( 2 ) ( PGE ( 2 ) ) production and cyclooxygenase ( COX ) gene expression in dental pulp cells , and ( ii ) pulp cells that express different prostaglandin E ( 2 ) receptor ( EP ) isoforms and their activation by PGE ( 2 ) leads to downstream Ca ( 2 + ) signalling . - pubmed
Expression of prostaglandin EP receptor isoforms was studied by RT - PCR , whereas fura-2 fluorescence was used to measure calcium mobilization . - pubmed
CONCLUSIONS : Interleukin-1beta and TNF - alpha may stimulate PGE ( 2 ) production in dental pulp cells . - pubmed
Activation of prostaglandin EP receptors in dental pulp cells by PGE ( 2 ) may induce Ca ( 2 + ) signalling to regulate cellular biological activity during inflammation . - pubmed
BACKGROUND : The integral synaptic vesicle protein and putative calcium sensor , synaptotagmin 1 ( STG ) , has also been implicated in synaptic vesicle ( SV ) recovery . - pubmed
The candidate biomarker was then obtained from tricine one - dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as Serum Amyloid A protein ( SAA ) by MALDI / MS - MS and database searching . - pubmed
Urinary trefoil factor 1 is a novel potent inhibitor of calcium oxalate crystal growth and aggregation . - pubmed
We searched for direct evidence to define the inhibitory activity of urinary trefoil factor 1 against calcium oxalate crystal growth . - pubmed
Moreover , we also evaluated whether urinary trefoil factor 1 has any effects on calcium oxalate crystal aggregation and transformation . - pubmed
MATERIALS AND METHODS : Isolated and aggregated forms of calcium oxalate monohydrate crystals were produced in the absence or presence of 7 , 70 and 700 ng / ml urinary trefoil factor 1 , nephrocalcin as a positive control or lysozyme ( Sigma - Aldrich ) as a negative control . - pubmed
RESULTS : The data clearly indicated that urinary trefoil factor 1 and nephrocalcin at physiological levels could effectively inhibit calcium oxalate monohydrate crystal growth and aggregation , whereas lysozyme did not affect the growth and aggregation of calcium oxalate monohydrate crystals . - pubmed
At a supraphysiological concentration of 4 microg / ml urinary trefoil factor 1 and nephrocalcin could transform calcium oxalate monohydrate crystals to the dihydrate type , which has much less adsorptive capability . - pubmed
CONCLUSIONS : To our knowledge these data provide the first direct evidence that urinary trefoil factor 1 is a novel potent inhibitor of calcium oxalate crystal growth and aggregation , and can transform calcium oxalate monohydrate crystals to the dihydrate type . - pubmed
Norepinephrine increased maternal heart rate ( HR ) , mean arterial pressure ( MAP ) and uterine vascular resistance ( R(UtA ) ) , and decreased uterine volume blood flow ( Q(UtA ) ) . - pubmed
The authors have investigated whether glaucoma and filtration surgery are associated with increased levels of vascular endothelial growth factor ( VEGF ) , and whether a humanized monoclonal antibody against VEGF , bevacizumab , can reduce postoperative scar formation and improve surgical outcome . - pubmed
The effect of VEGF and bevacizumab on Tenon fibroblasts in vitro was determined using a proliferation assay . - pubmed
Fibroblast proliferation in vitro was stimulated by delivery of VEGF , and was inhibited by administration of bevacizumab . - pubmed
The helical propensity of a peptide corresponding to beta2 - strand of salivary SA cystatin analyzed by CD display high helical propensity in aqueous solution , whereas peptides matching the beta2 - strand amino acid sequence of cystatins S and SN , display random coil conformation in aqueous solution but acquire alpha - helical conformation in the presence of trifluoroethanol ( TFE ) . - pubmed
The specific inhibitor of VAP-1 , UV-002 , was administered by daily i.p . - pubmed
,effects on insulin secretion and sensitivity and blood glucose levels were assessed by means of a hyperglycaemic glucose clamp , meal tolerance test , homeostasis model assessment - insulin resistance score , and daily self - monitoring of blood glucose . - pubmed
RESULTS : Palosuran did not affect second - phase insulin response ( primary end - point ) during the hyperglycaemic glucose clamp in comparison with placebo [ paired difference of -1.8 microU ml(-1 ) , 95 % confidence interval ( CI ) -7.8 , 4.2 ] . - pubmed
Likewise , no effects of palosuran were detected on the first - phase insulin response , or on insulin secretion and blood glucose levels during the meal tolerance test or on homeostasis model assessment - insulin resistance score . - pubmed
The fingerprinting atlas indicated that , compared with those without SCI , the SCI group demonstrated the following characteristics with regard to second principal component : it is made up of fatty acids , myc - inositol , arginine , very low - density lipoprotein ( VLDL ) , low - density lipoprotein ( LDL ) , triglyceride ( TG ) , glucose , and 3 - methyl - histamine . - pubmed
Both mitochondrial membrane potential ( MMP ) and oxygen consumption were assessed in conjunction with quantification of mitochondrial DNA and mitochondrial biogenesis - related factors , such as peroxisome proliferator - activated receptor - gamma coactivator 1 alpha ( PGC1alpha ) , nuclear respiratory factor 1 ( NRF1 ) and mitochondrial transcription factor A ( Tfam ) , by real - time polymerase chain reaction . - pubmed
[ Effect of rapamycin combined with cisplatin on head and neck squamous cancer cells regulated by CCL19 ] . - pubmed
OBJECTIVE : To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine ( C - C motif ) ligand 19 ( CCL19 ) . - pubmed
RESULTS : Rapamycin and cisplatin could respectively increase the growth arrest , the proportion of G(1 ) phase and apoptosis of CCL19 induced cancer cells ( P & lt ; 0.05 ) . - pubmed
AIM : The aim of this study was to assess a spray formulation containing colloidal silver and hyaluronic acid . - pubmed
The fact that the K(m ) of OTxA for the efflux transporter hNPT4 was much higher than those for the uptake transporters hOAT1 and hOAT3 may favor the accumulation of OTxA in the tubular cell and lead to nephrotoxicity . - pubmed
The results indicated that : ( 1 ) the decay in fluorescence was linearly dependent on the concentration of NO ( 2 ) , indicating that protein oxidation was first order with respect to NO ( 2 ) concentration in both BALF and in buffered albumin solution ; ( 2 ) the depletion kinetics of protein in BALF was non - linear with respect to substrate concentration ; ( 3 ) the rate of protein depletion was much slower in BALF than in a buffered solution of albumin , suggesting that the presence of antioxidants in BALF protected proteins from being oxidized by NO ( 2 ) ; and ( 4 ) whereas the addition of ascorbic acid to buffered albumin solution significantly attenuated albumin depletion , the addition of glutathione had no effect . - pubmed
For gp120 sequence analysis , nucleotide mixtures were considered when the second highest electropherogram peak was & gt;25 % ; sequences were translated into all possible permutations and classified as X4 , dual / mixed ( DM ) , and R5 based on coincident ESTA results . - pubmed
According to multivariate analysis , amino acid changes at 9 and 7 positions within the C1 to C4 and V1 to V5 regions , respectively , maintained a statistical significance , as did the net charge of V3 and C4 . - pubmed
Furthermore , using quantitative electrophoretic mobility - shift assays we validated the positional interdependence of two nucleotides and the presence of a secondary Sox motif in the affinity landscape of Sox4 . - pubmed
These results suggest that a concerted rearrangement of two interface amino acids enables Sox4 to accommodate primary and secondary motifs . - pubmed
Phosphorylation of mycobacterial PcaA inhibits mycolic acid cyclopropanation : consequences for intracellular survival and for phagosome maturation block . - pubmed
Here , we demonstrated that mycolic acid cyclopropane synthase PcaA , but not MmaA2 , was phosphorylated by mycobacterial Ser / Thr kinases at Thr-168 and Thr-183 both in vitro and in mycobacteria . - pubmed
In contrast to the wild - type and phosphoablative pcaA alleles , introduction of the phosphomimetic pcaA allele in the DeltapcaA mutant failed to restore the parental mycolic acid profile and cording morphotype . - pubmed
Chronic treatment with AG825 or AG1478 , selective inhibitors of erbB2 and EGFR respectively , did not affect hyperglycemia but led to an exacerbation whereas acute administration of the EGFR ligand , epidermal growth factor ( EGF ) , led to an improvement in cardiac recovery in diabetic hearts . - pubmed
Losartan treatment improved cardiac function in diabetes but also impaired EGFR phosphorylation in diabetic heart . - pubmed
Co - administration of EGF rescued Losartan - mediated reduction in EGFR phosphorylation and significantly improved cardiac recovery more than with either agent alone . - pubmed
The sensor responded to maltose and maltotriose and the response was completely abolished by introduction of a single point mutation in the BRET(2 ) tagged MBP protein . - pubmed
The biosensor 's estimate of maltose in beer matched that of a commercial enzyme - linked assay but was quicker and more precise , demonstrating its applicability to real - world samples . - pubmed
kansui led to the isolation of 12 diverse terpenoids whose structures were identified by ( 1 ) H , ( 13 ) C NMR spectroscopy and ESI - MS as kansuinine A ( 1 ) , kansuinine B ( 2 ) , kansuinine C ( 3 ) , kansuiphorin C ( 4 ) , 3 - O -( 2'E,4'Z - decadienoyl )- 20 - O - acetylingenol ( 5 ) , 3 - O -( 2'E,4'Edecadienoyl )- 20 - O - acetylingenol ( 6 ) , 3 - O -( 2'E,4'Z - decadienoyl )- 20 - deoxyingenol ( 7 ) , 3 - O - benzoyl-20 - deoxyingenol ( 8) , 5 - O - benzoyl-20 - deoxyingenol ( 9 ) , kansenone ( 10 ) , epi - kansenone ( 11 ) , euphol ( 12 ) . - pubmed
Specifically , we report formulation optimisation of SLMH microparticles and tablets using coumarin 102 ( log P = 4.09 ) as a model Biopharmaceutics Classification System class II drug . - pubmed
Binding of the antibody to bone marrow - derived mast cells triggered activation events that included cell degranulation , Ca ( 2 + ) response , dephosphorylation of ezrin / radixin / moesin ( ERM ) family proteins , and potent tyrosine phosphorylation of the non - T cell activation linker ( NTAL ) but only weak phosphorylation of the linker for activation of T cells ( LAT ) . - pubmed
To construct such an in situ amplification system , streptavidin - labeled gold nanoparticles ( AuNP - SA ) were initially used for the labelling of initiator strands ( S0 ) and detection antibody ( mAb2 ) with a large ratio ( mAb2 - AuNP - S0 ) , and then two auxiliary DNA strands S1 and S2 were designed for in situ propagation of DNAzyme concatamers with the hemin / G - quadruplex format . - pubmed
Myocardial blood flow and high - energy nucleotides were obtained in the LAD region and normalized to remote regions . - pubmed
Despite reduced regional blood flows in HIB hearts , ATP content in the LAD region , when normalized to the remote region was similar in HIB versus SWOP ( 1.06 + -0.06 and 1.02 + -0.05 respectively ; NS ) as was the transmural phosphocreatine ( PCr ) to ATP ratio ( 2.1 + -0.2 and 2.2 + -0.2 respectively ; NS ) . - pubmed
Emerging low - density lipoprotein ( LDL ) therapies : Management of severely elevated LDL cholesterol -- the role of LDL - apheresis . - pubmed
Based on electrochemical principles , it selectively removes apolipoprotein B - containing lipoproteins through extracorporeal precipitation with either heparin ( Heparin - induced Extracorporeal LDL Precipitation , ie , HELP ) or dextran sulfate ( Liposorber ) . - pubmed
LDL - apheresis can lead to an acute decrease in LDL cholesterol ( LDL - C ) of 70%-80 % , but there is a rapid rebound to baseline levels within approximately 2 weeks . - pubmed
Reducing the production cost of cellulase as the key enzyme for cellulose hydrolysis to fermentable sugars remains a major challenge for biofuel production . - pubmed
The requirement for long - lasting artificial ventilation can be reduced with an oxime therapy that is optimized by using the cholinesterase status for guidance or by measures ( e.g. - pubmed
CONCLUSION : Our economic analysis shows that although the costs of the two trastuzumab plus taxane regimens are similar , they may contribute to the on - going debate about the availability and use of innovative chemotherapy drugs , in particular in human epidermal growth factor receptor 2 - positive metastatic breast cancer with new therapies such as trastuzumab - DM1 and pertuzumab . - pubmed
miR-181b restricted cAMP production by post - transcriptionally downregulating AC9 expression . - pubmed
Herein , we have successfully formulated insoluble iodinated cisplatin ( CDDP - I ) into an LPI NPs ( lipid - coated iodinated CDDP nanoparticles ) . - pubmed
On days 14 , 28 and 42 of exposure to ZEN ( 100 mug kg(-1 ) feed day(-1 ) ) , apoptosis in IPP GC was evaluated histologically in HE - stained specimens , immunohistochemically by active caspase-3 staining and in mononucleosome and oligonucleosome detection - based ELISA . - pubmed
Proliferation was evaluated histologically by mitosis detection in HE - stained specimens , immunohistochemically by PCNA staining and in the MTT tetrazolium salt colorimetric assay detecting mitogenic responses of B cells to LPS . - pubmed
Exposure to low doses of ZEN for several weeks intensified apoptosis and weakened proliferation in IPP lymphocytes . - pubmed
ZEN influences gut - associated lymphoid tissue ( GALT ) by decreasing the expression of CD21 + on B cells and by increasing the percentage of B1 cell populations . - pubmed
Tiotropium is a muscarinic receptor antagonist , which is taken once daily , in maintenance therapy , in every stage of the disease progress . - pubmed
The putative diol synthase from G. cingulate was purified by His - Trap affinity chromatography with a specific activity of 0.87 U mg(-1 ) , an eightfold purification , and a yield of 28 % . - pubmed
One unit of activity was defined as the amount of enzyme required to produce 1 mumol of 7,8 - dihydroxy-9,12 ( Z , Z )- octadecadienoic acid ( 7,8 - DiHODE ) per min . - pubmed
The enzyme converted linoleic acid to a product , identified as 7S,8S - DiHODE by liquid chromatography - mass spectrometry / mass spectrometry ( LC - MS / MS ) and nuclear magnetic resonance ( NMR ) spectroscopy . - pubmed
The specific activity and catalytic efficiency ( k cat / K m ) of 7,8 - diol synthase from G. cingulate for the conversion of fatty acid to dihydroxy fatty acid followed the order linoleic acid & gt ; alpha - linolenic acid & gt ; oleic acid & gt ; palmitoleic acid , indicating that the enzyme is a 7,8 - linoleate diol synthase ( 7,8 - LDS ) . - pubmed
The activity of the enzyme for the conversion of 7,8 - DiHODE from linoleic acid was maximal at pH 6.5 , 40 C , and 2.5 % ( v / v ) dimethyl sulfoxide ( DMSO ) . - pubmed
The accumulation of 8 - HPODE was due to a higher 8 - dioxygenase activity in the N - terminal domain than hydroperoxide isomerase activity in the C - terminal domain . - pubmed
Stabilization of HIF-1alpha by FG-4592 promotes functional recovery and neural protection in experimental spinal cord injury . - pubmed
Combination therapy including the specific HIF-1alpha blocker YC-1 down - regulated the HIF-1alpha expression and partially abolished the protective effect of FG-4592 . - pubmed
Taken together , our results revealed that the role of FG-4592 in SCI recovery is related to the stabilization of HIF-1alpha and inhibition of apoptosis . - pubmed
Modulation of Cav3.2 T - type calcium channel permeability by asparagine - linked glycosylation . - pubmed
Notably , Lys47 , a key residue in the bioactive peptide lactoferricin , was particularly susceptible to modification . - pubmed
The second study was a Phase II dose - finding study of senicapoc ( a Gardos channel blocker ) compared to placebo . - pubmed
Compared to the placebo group the high dose senicapoc showed significant improvement in change in hemoglobin level , number and proportion of dense red blood cells , red blood cell count and indices and hematocrit . - pubmed
While the Phase II and the prematurely terminated phase III studies of senicapoc showed that the drug improved red cell survival ( depending on dose ) , this did not lead to fewer painful crises . - pubmed
In most cases , the anti - TNF drug involved was etanercept . - pubmed
In 71 cases there was at least a partial resolution by discontinuation of the anti - TNF treatment , initiation of steroids or both . - pubmed
A 90 - day repeated - dose oral toxicological evaluation was conducted according to GLP and OECD guidelines on the methylurate purine alkaloid theacrine , which is found naturally in certain plants . - pubmed
Site - directed mutagenesis of eight transcription factor binding sites within PGhGDSL demonstrated that the MYB1AT motif ( AAACCA ) at -603/-598 was critical for the 19 DPA - specific reporter gene expressions . - pubmed
Analysis of genes co - expressed with GhMYB1 showed that it potentially regulates a number of other 19 - 25 DPA - specific genes in networks including those functioning in the cell wall and precursor synthesis , but not the major polysaccharide and protein components of the fibre SCW . - pubmed
The capabilities of C18 and styrene divinylbenzene reversed - phase sulfonated ( SDB - RPS ) Empore disks for capturing non - polar and polar metabolites , respectively , were compared . - pubmed
High - sucrose diet ( HSD ) fed adult females developed systemic insulin resistance and exhibited an ovarian phenotype characterized by excess accumulation of lipids and cholesterol in the ovary , decreased ovary size , and impaired egg maturation . - pubmed
In this study , we prepared alginate microparticles loaded with the model antigen fluorescein isothiocyanate conjugated - bovine serum albumin . - pubmed
The use of ovarian suppression combined with an aromatase inhibitor exemestane for 5 years , resulted in further reduction of recurrence . - pubmed
The results revealed drug - dependent and vegetable - dependent pharmacokinetic differences and indicated that ephemeral vegetables could have high capacity accumulating antibiotics ( up to 160 mug g-1 for TC and 38 mug g-1 for SMX ) within hours . - pubmed
Based on the volume of distribution ( Vss ) , SMX was 3 - 6 times more extensively distributed than TC . - pubmed
Detailed analyses revealed that the mTORC1 and p53 signaling pathways are main targets of curcumin . - pubmed
In this study , the effect of therapeutic hypervolemia , hemodilution , and hypertension ( HHH - therapy ) on cerebral blood flow ( CBF ) was assessed by xenon - enhanced computerized tomography ( XeCT ) hypothesizing an increase in CBF in poorly perfused regions . - pubmed
CBS treatment also significantly decreased the levels of triglyceride , total cholesterol , and free fatty acid in the liver . - pubmed
The activity of superoxide dismutase in the liver was increased after treatment with CBS , however , levels of malondialdehyde and reactive oxygen species decreased . - pubmed
The expression of fatty acid synthase , nuclear factor kappa - light - chain - enhancer of activated B cells , Cysteinyl aspartate - specific proteinase-3 , and synonyms B - cell leukemia / lymphoma-2 gene - associated X protein were downregulated after treatment with CBS , whereas the expression of nuclear factor erythroid-2 - related factor 2 was upregulated . - pubmed
Bioinspired , Manganese - Chelated Alginate - Polydopamine Nanomaterials for Efficient in Vivo T1 - Weighted Magnetic Resonance Imaging . - pubmed
Hence , the T1 relaxivity measurement showed that PDA - complexed synthetic NGs reveal a strong positive contrast enhancement with r1 = 12.54 mM-1 s-1 in 7.0 T MRI images , whereas DA - complexed synthetic NGs showed a relatively lower T1 relaxivity effect with r1 = 10.13 mM-1 s-1 . - pubmed
Furthermore , T1 - weighted MRI results revealed that PEGylated AlgPDA(Ca / Mn ) NGs significantly enhanced the signals from liver and tumor tissues . - pubmed
Therefore , owing to the enhanced permeability and retention effect , significantly enhanced in vitro and in vivo imagings , low cost , and one - pot synthesis method , the Mn - based biomimetic approach used in this study provides a promising and competitive alternative for noninvasive tumor detection and comprehensive anatomical diagnosis . - pubmed
Subsequent periodic acid - Schiff ( PAS ) staining after diastase digestion confirmed GH . - pubmed
In this context , we have developed a hypothesis that describes the evolution of schizophrenia - from the premorbid through the prodromal stages to syndromal psychosis - and posits dysregulation of glutamate neurotransmission beginning in the CA1 region of the hippocampus as inducing attenuated psychotic symptoms and initiating the transition to syndromal psychosis . - pubmed
The curcuminoid - imprinted polymer ( Cur - MIP ) was synthesized from 0.0237 mmol of styrene , 47.0 g of acetonitrile , 1.0238 mmol of ethylene glycol dimethacrylate , 0.0325 mmol of curcuminoids , and 0.2480 mmol of benzoyl peroxide . - pubmed
The sample solution was separated using the Cur - MIP via solid - phase extraction and analyzed on a Brownlee analytical C18 column ( 150 mm x 6 mm , 5 microm ) using an isocratic elution consisting of acetonitrile and 0.1 % trichloroacetic acid ( 40:60 , v / v ) . - pubmed
Thus , we concluded that the Cur - MIP and high - performance liquid chromatographic - fluorescence method could be applied to selective extraction and could be used as a rapid tool for the determination of curcuminoids in medicinal herbal extracts . - pubmed
In this study , we demonstrated that there are large species differences in the kinetics of interactions of a key class of antiviral drugs , acyclic nucleoside phosphonates ( ANPs ) , with organic anion transporter 1 [ ( OAT1 ) SLC22A6 ] and identified a key amino acid residue responsible for these differences . - pubmed
Chimeric and site - directed mutagenesis studies along with comparative structure modeling identified serine at position 203 ( S203 ) in hOAT1 as a determinant of its lower Km value . - pubmed
The studied parameters were air pollutants , ascorbic acid , photosynthetic pigments , leave 's pH , relative water content , proline , carbohydrates , and hydrogen peroxide , in three chosen spots , near the pollution source ( P1 ) , opposite the latter spot ( P2 ) , and in an area relatively far from the source of contamination and which was chosen as the control site ( Ctrl ) . - pubmed
The results showed in P1 detection of air pollutants higher of about 80 % than in Ctrl , modifications in stress markers : increased concentration of the reactive oxygen species " hydrogen peroxide , " rise in the concentration of the osmoregulator amino acid " proline , " and depletion in chlorophyll content , in contrast to an increase in pheophytin . - pubmed
Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells : A preliminary study . - pubmed
The present study aimed to observe the expression of circadian gene clock circadian regulator ( CLOCK ) in ovarian cancer cells and the effects of circadian gene CLOCK on cis - dichlorodiamine platinum ( cisplatin ) resistance in ovarian cancer cells . - pubmed
The expression of circadian gene CLOCK in ovarian cancer cells was strongly associated with cisplatin resistance . - pubmed
The upregulation of circadian gene CLOCK in ovarian cancer cells may reduce its sensitivity to cisplatin treatment . - pubmed
Imatinib , a small molecule tyrosine kinase inhibitor , induces rapid and sustained clinical benefit by inhibiting a number of signaling pathways , including BCR - ABL and c - KIT . - pubmed
RESULTS : There were 791 patients with the following characteristics : mean age 56.2 , male 52.3 % , known diabetic 61.6 % , on insulin 46.1 % , mean initial glucose 50.0 dl / mg ( 2.8 mmol / L ) , from home 56.3 % . - pubmed
Results : In HFD maternal plasma / serum , concentrations of glucose , beta - hydroxybutyrate , branched - chain amino acids , and leptin were increased , whereas those of triacylglycerol , cholesterol , and palmitic , oleic , linoleic , and alpha - linolenic acids were decreased . - pubmed
Two new indium sulfate tellurites , namely , In2 ( SO4 )( TeO3 )( OH ) 2 ( H2O ) and In3 ( SO4 )( TeO3 ) 2F3 ( H2O ) , were synthesized by hydrothermal method in a one - pot reaction . - pubmed
In2 ( SO4 )( TeO3 )( OH ) 2(H2O ) crystallized in centrosymmetric ( CS ) space group P21 / n , while In3 ( SO4 )( TeO3 ) 2F3 ( H2O ) formed a non - centrosymmetric ( NCS ) and chiral space group P212121 . - pubmed
The CS compound features a 2D layered structure composed of 2D indium oxide layers decorated by sulfate tetrahedra and tellurite groups . - pubmed
Liver inflammation was assessed by plasma sCD163 and sMR levels ( ELISA ) , fibrosis by liver stiffness ( transient elastography ) , function by galactose elimination capacity ( GEC ) , and cognitive performance by continuous reaction time ( CRT ) . - pubmed
During DAA - therapy , we observed a rapid sCD163 decline from baseline to end of treatment ( EOT ) ( 6.9 vs. 3.8 mg / L , p<0.0001 ) , whereas the change in sMR was more subtle ( 0.37 vs. 0.30 mg / L , p<0.0001 ) . - pubmed
Moreover , we found that the putative cation - binding site of TMEM180 is conserved among orthologs , and that its position is similar to that of melibiose transporter MelB. - pubmed
BACKGROUND : There is great interest in understanding whether interventions on sugar - sweetened beverage ( SSB ) consumption through pregnancy and early childhood affect adolescent body mass index ( BMI ) . - pubmed
Results : The PEG - Lipo - beta - E showed good stability and was characterized as 83.31 + - 0.181 nm in size , 0.279 + - 0.004 in polydispersity index ( PDI ) , -21.4 + - 1.06 mV in zeta potential , 6.65 + - 0.02 in pH , 5.024 + - 0.107 mg / mL in beta - elemene ( beta - E ) content , and 95.53 + - 1.712 % in average EE . - pubmed
We previously obtained the AcCHMOM6 mutant , which oxidizes omeprazole sulfide ( OPS ) to the chiral sulfoxide drug esomeprazole . - pubmed
To further improve the catalytic efficiency of the AcCHMOM6 mutant , a focused mutagenesis strategy was adopted at the intersections of the FAD - binding domain , NADPH - binding domain and alpha - helical domain based on structural characteristics of AcCHMO . - pubmed
AcCHMOM7 ( L55Y / P497S ) was obtained by combining the two mutations , which increased the specific activity from 18.5 ( M6 ) to 108 U / g , and an increase in the K i of the substrate OPS from 34 to 265 muM. - pubmed
Many BDA - labeled axonal fibers and terminals from the OPC were ipsilaterally observed in the rostral and rostroventral regions of the primary somatosensory cortex ( S1 ) , the rostral region of the secondary somatosensory cortex ( S2 ) , and the most rostrocaudal levels of the granular insular cortex ( GI ) . - pubmed
Furthermore , injections of a retrograde tracer , Fluorogold , into these S1 , S2 , and GI regions , resulted in preferential labeling of neurons in the ipsilateral OPC among the intralaminar and sensory thalamic nuclei . - pubmed
The steady - state levels of intracellular cyclic AMP were higher than those observed in unstimulated cells , and cyclic AMP - dependent protein phosphokinase was in a highly activated state as compared to controls . - pubmed
In contrast , inhibition of growth , apparent cell death , and unusual morphological changes were observed in both normal and transformed cells when high concentrations of either PGE1 ( 10 micron ) or the phosphodiesterase inhibitor 1 - methyl , 3 - isobutylxanthine ( 0.5 mM to 2 mM ) were used , which was indicative of toxic effects of the drugs . - pubmed
Ribulose diphosphate carboxylase was present in the methanol - bicarbonate - grown organism at six times the specific activity of that in the succinate - grown organism . - pubmed
Reactivity of two anti - galactosyl ceramide antibodies towards myelin basic protein . - pubmed
Two anti - galactosyl ceramide antibodies ( polyclonal Ab142 and a monoclonal antibody ) were characterized in terms of their reactivity towards purified lipids and myelin basic protein . - pubmed
Binding of MAb - Gal C to Gal - C was equally inhibited by ceramide and galactose to approximately 50 % , indicating that both groups are important for antibody recognition . - pubmed
MAb - Gal C was also shown to be reactive with the structurally related lipids , sphingomyelin and sulfatide . - pubmed
Polyclonal Ab142 , although raised against Gal C , was shown to be 3 - fold more reactive with component 8 ( C-8 ) of myelin basic protein than Gal C. On the other hand , the MAb - Gal C which also reacted with C-8 was 2 - fold less reactive with C-8 than with Gal C. Neither of these antibodies were reactive with component 1 ( C-1 ) of myelin basic protein . - pubmed
An anti - MBP IgG was shown to be reactive with C-1 and C-8 but unreactive with Gal C. In competitive inhibition ELISA , C-8 was able to compete out 44 % and 41 % of Gal C binding to polyclonal Ab142 and MAb , respectively . - pubmed
D - galactose was unable to inhibit C-8 binding to either antibody , whereas ceramide was as efficient as Gal C.(ABSTRACT TRUNCATED AT 250 WORDS ) - pubmed
Anti -( 14 - 3 - 3 protein ) antibody inhibits stimulation of noradrenaline ( norepinephrine ) secretion by chromaffin - cell cytosolic proteins . - pubmed
The 9 trans , 12 cis isomer of linoleic acid is a poor substrate and the 9 cis , 12 trans isomer is a good substrate for the enzyme that desaturates dienoic acids at position six . - pubmed
The therapeutic application of recombinant NEP protects the airways from the adverse actions of stimuli that release inflammatory peptides , and induction of the NEP gene expression by glucocorticoids suggest a possible mechanism for the action of these steroids in treating airway diseases such as asthma , chronic bronchitis , or cystic fibrosis . - pubmed
The method involves the first dimension electrophoresis in agarose gel containing concanavalin A ( Con A ) or lentil lectin ( LCH ) , the second dimension immunoelectrophoresis in agarose gel containing polyclonal antibody against AFP , reaction with peroxidase - conjugated Protein A and staining with 4 - methoxy-1 - naphthol . - pubmed
Binding to Site 1 was characterized by : being displaced by dextran sulphate or heparin ; being dependent on Ca2 + ; having a Kd value of about 15 micrograms of protein / ml ; not being significantly displaced by a 20 - fold excess unlabelled low - density lipoprotein that had been reductively methylated ; being displaced by approx . - pubmed
As much as 60 % of this exonuclease activity was similar to that associated with DNA polymerase delta based upon its sensitivity to inhibition by GMP and by butyl - phenyl - deoxyguanosine triphosphate ( BuPdGTP ) . - pubmed
Association of 3 '---- 5 ' exonuclease activity with the DNA polymerase in the replitase complex was also demonstrated by analyzing dTTP turnover to dTMP in an in vitro DNA polymerase assay system . - pubmed
The DNA polymerase activity in replitase complex exhibited a sensitivity to BuPdGTP which both was similar to that of DNA replication in permeable cells and was intermediate between the BuPdGTP inhibition of purified DNA polymerases alpha and delta . - pubmed
A hormone - dependent dissociation of hsp90 from the cytosolic form of the receptor complex was observed within the first hour of in vivo progesterone treatment , which could explain the lack of hsp90 in nuclear receptor complexes . - pubmed
In a cell - free system , hsp90 binding to receptor was stabilized by molybdate but disrupted by high salt . - pubmed
The anorectic effect of 4 mg kg-1 quipazine was antagonized by the nonselective 5 - HT - receptor antagonist methysergide ( 5 mg kg-1 , i.p . ) - pubmed
and by the selective 5 - HT2 - receptor antagonists ketanserin ( 1 mg kg-1 and 2.5 mg kg-1 , i.p . ) - pubmed
The selective 5 - HT3 - receptor antagonist GR38032F ( 1 mg kg-1 , i.p . ) - pubmed
, which blocks some of the effects mediated at 5 - HT1 - like receptors , did not block the reduction in food intake produced by this dose of quipazine . - pubmed
None of the 5 - HT - receptor antagonists had any effect on food intake when they were administered alone , suggesting that endogenous 5 - HT is not involved in the tonic control of food intake under the conditions of these experiments . - pubmed
It is concluded that the anorectic action of quipazine is mediated , at least in part , by activation of 5 - HT2 - receptors . - pubmed
The 32P incorporation rate into phospholipids ( PC , phosphatidylinositol ( PI ) , phosphatidylethanolamine ( PE ) ) was affected basicly different analyzing either PC , PI or PE . - pubmed
Histamine and propranolol provoked the highest incorporation of 32P ( 240 % increase in PI labeling ) . - pubmed
Isoproterenol and adrenalin decreased PC labeling ( 45 % and 18 % ) whereas isoproterenol decreased 32P incorporation into PI ( 18 % ) , and adrenalin led to an increase ( 37 % ) . - pubmed
A decrease in hexadecanoic acid content of PI was found after administration of either isoproterenol , adrenalin or histamine . - pubmed
The tumor shows tubular structures with hyaluronic acid , " retinal rosettes " , and areas with ganglion and GFAP - positive glial cells . - pubmed
The detection of venous thrombosis of the legs using 125 - I - labelled fibrinogen . - pubmed
Intravertebral artery injection of rauwolscine , 5 micrograms enhanced the BCO response but had no effect on basal blood pressure . - pubmed
It is concluded that enhancement of the BCO response by yohimbine and rauwolscine is caused by blockade of central alpha 2 - adrenoceptors . - pubmed
Platelet density distribution after isopycnic centrifugation on a discontinuous stractan density gradient , dense granule markers ( serotonin , ATP and ADP ) and alpha granule markers ( intraplatelet beta - thromboglobulin and platelet factor 4 ) were simultaneously determined . - pubmed
We have found that the gamma - carboxyglutamic acid ( GLA)-containing protein from bone ( BGP , osteocalcin ) has chemotactic activity in vitro for a number of cells which are found adjacent to endosteal bone surfaces in vivo . - pubmed
A synthetic peptide ( Phe - Tyr - Gly - Pro - Val ) , which is identical to the carboxy - terminal peptide cleaved from BGP when digested by trypsin , is also chemotactic for the same cells . - pubmed
BGP retains its chemotactic activity after conversion of the gamma - carboxyglutamic acid residues to glutamic acid , indicating that this biological effect requires neither gamma - carboxyglutamate nor the ability of BGP to bind calcium . - pubmed
Computer analysis of the data for total radioactivity ( PCNU equivalents ) , based upon an open two - compartment model , yielded values of the pharmacokinetic parameters K12 , K21 , and K10 of 1.49 hr-1 , 0.25 hr-1 , and 0.19 hr-1 , respectively . - pubmed
In the groups receiving 8.2 of omega 3 fatty acids , there was a significant decrease in serum triglyceride and very low - density lipoprotein levels , which is in accord with earlier observations . - pubmed
In the other parameters , including cholesterol and high - density lipoprotein cholesterol , no decrease or increase was observed . - pubmed
Identification of major tyrosine - phosphorylated proteins in Csk - deficient cells . - pubmed
Csk is a non - receptor protein - tyrosine kinase that acts as a negative regulator of Src family tyrosine kinases . - pubmed
In these embryos , an accumulation of tyrosine phosphorylated proteins was observed as a consequence of constitutive activation of Src family kinases . - pubmed
In order to identify those tyrosine phosphorylated proteins , we established a Csk - deficient cell line from embryos lacking both Csk and the anti - oncogene product p53 . - pubmed
It also induces in parallel the early tyrosine phosphorylation of numerous proteins , including phospholipase C gamma-1 . - pubmed
A marked conversion of the T cell - specific tyrosine kinase p56lck to a slow migrating 60 - kDa form is also found following GM1 addition . - pubmed
wts of 14,500 and 15,000 , which slightly differ in their isoelectric point ( 4.9 and 5.3 ) and amino acid composition . - pubmed
Low serum albumin and the increased risk of amikacin nephrotoxicity . - pubmed
Furthermore , low serum albumin concentration was also associated with amikacin accumulation in plasma . - pubmed
The group of patients with hypoalbuminemia ( & lt ; or = 3.0 g / dL ) had a significantly higher trough amikacin plasma level ( assessed at 48 and 96 hours of the initiation of treatment ) than those with normal serum albumin , with no differences among the age , sex , baseline renal function and received amikacin doses . - pubmed
CONCLUSIONS : We conclude that serum albumin concentration is the most powerful predictor of amikacin nephrotoxicity . - pubmed
The data suggest that a structural feature of FGF3 involving the amino - terminal region and glycosylation site has a significant bearing on its passage through the Golgi complex and may regulate the secretion of the ligand . - pubmed
Condensed chromatin can form protruding patches , which eventually bud from the nucleus in sealed vesicles through a process which is actin - dependent , since it could be blocked by cytochalasins . - pubmed
Antigen levels of tissue plasminogen activator ( t - PA ) , urokinase - type plasminogen activator ( u - PA ) , plasminogen activator inhibitors type 1 ( PAI-1 ) and PAI-2 in GCF were determined with ELISAs and 17 beta - oestradiol and progesterone in serum with radioimmunoassays . - pubmed
A statistically significant correlation between delta progesterone and delta PAI-2 was noted : the higher delta progesterone , the lower delta PAI-2 . - pubmed
Antibody quantitation ( anti - D ) had a positive predictive value of 54.5 per cent and negative predictive value of 85.7 per cent while MMA had a positive predictive value of 75 per cent and a negative predictive value of 100 per cent . - pubmed
Methotrexate is partly oxidised by hepatic aldehyde oxidase to 7 - hydroxymethotrexate . - pubmed
A gene transcribed from the bidirectional ATM promoter coding for a serine rich protein : amino acid sequence , structure and expression studies . - pubmed
In an earlier report we showed that the 5 ' end of the gene for ataxia telangiectasia ATM is within 700 bp of the 5 ' end of a novel gene E14 , and suggested that the CpG island that separates these genes functions as a bidirectional promoter . - pubmed
We have now determined the complete amino acid sequence of the E14 protein , defined the exon / intron structure of the gene and estimate that the complete gene is more than 55 kb in length . - pubmed
The compound under study in serum samples was extracted with Sep - Pak C18 cartridge and the extract was applied to an octadecylsilane - bonded column ( Nucleosil 5C18 , 150 x 4 mm I.D. ) - pubmed
Disturbances in neuronal calcium homeostasis may result in the activation of several calcium - sensitive enzymes , including lipases , kinases , phosphatases , and proteases . - pubmed
One potential pathogenic event in a number of acute CNS insults , including TBI , is the activation of the calpains , calcium - activated intracellular proteases . - pubmed
AIM : To investigate separately the effects of sucralfate on acute gastric and duodenal injury and on changes in intragastric bleeding induced by aspirin . - pubmed
In this report , using biotinylated dextran amine ( BDA ) and cholera toxin subunit B ( CTB ) for anterograde and retrograde labelling , respectively , we investigated projections from the nBOR and LM to the dorsal thalamus . - pubmed
Two regression models were developed that were based on the age - dependent , relative abundance of 2 cuticular hydrocarbons , pentacosane ( GC peak 1 ) and nonacosane ( GC peak 5 ) . - pubmed
Two new regression models were developed that also use the relative abundance of a 3rd cuticular hydrocarbon , octacosane ( GC peak 4 ) . - pubmed
Furthermore , an inhibitor of the hypusine modification , GC7 ( N ( 1 )- guanyl-1 , 7 - diaminoheptane ) , prevents CD83 surface expression by apparently interfering with nucleocytoplasmic translocation of the CD83 mRNA and , importantly , significantly inhibits DC - mediated T lymphocyte activation . - pubmed
The data presented suggest that CD83 mRNA is transported from the nucleus to the cytoplasm via a specific nuclear export pathway and that hypusine formation appears to be essential for the maturation of functional DCs . - pubmed
ET-1 promotes the apoptosis through an increase in the intracellular calcium in the CL cells and affects the physiological function of CL cells by inhibiting progesterone production . - pubmed
It is concluded that reductions in renal Cu contents caused by Cd or LPS administration may be a result of Cd and NO displacement of Cu previously bound to metallothionein . - pubmed
Postoperative data I ( first follow - up : n = 66 ): sinus rhythm ( yes ): 4/7 vs. 47/59 ( NS ) ; pacemaker ( PM ) in AAI mode ( yes ): 1/7 vs. 3/59 ( NS ) ; inducible atrial fibrillation ( yes ): 2/7 vs. 5/59 ( NS ) ; Bradycardie Tachycardie Syndrome with the need of PM implantation 0/7 vs. 4/59 ( NS ) . - pubmed
Postoperative data II ( second follow - up : n = 55 ): sinus rhythm ( yes ): 5/7 vs. 34/48 ( NS ) ; PM in AAI mode ( yes ): 0/7 vs. 4/48 ( NS ) ; Inducible atrial fibrillation ( yes ): 2/7 vs. 5/48 ( NS ) ; Bradycardia Tachycardia Syndrome with the need of PM Implantation 0/7 vs. 5/48 . - pubmed
All serum samples were analyzed initially by fluorescence polarization immunoassay ( FPIA , TDx ) ( Abbot Diagnostics ; Irving , TX ) and again by enzyme multiplied immunoassay ( EMIT Vancomycin Assay ) ( Dade Behring ; San Jose , CA ) . - pubmed
Mapping the ligand - binding site on the C5a receptor : arginine74 of C5a contacts aspartate282 of the C5a receptor . - pubmed
Mutation of D(282 ) to A decreases the sensitivity of the receptor to activation by intact C5a but not by its less potent metabolite , C5adR(74 ) , which lacks the C - terminal arginine(74 ) . - pubmed
RESULTS : Propofol inhibited both IL-6 and IL-10 production at 0.5 microg / mL , 5 microg / mL and 50 microg / mL. - pubmed
Conversely , thiopental induced IL-10 production at 2 microg / mL and 20 microg / mL. - pubmed
CONCLUSION : Propofol appears to inhibit both IL-6 and IL-10 production by LPS - stimulated PBMCs in vitro . - pubmed
Identification of the functionally important cysteinyl residue in pig heart aspartate aminotransferase . - pubmed
Thalidomide has antiangiogenic and immunomodulatory properties and is an effective inhibitor of TNF - alpha . - pubmed
CONCLUSIONS : Detection of p53 gene mutation with PCR - SSCP - silver stain combined with cytological examination of sputum samples can improve the diagnosis of lung cancer . - pubmed
Preclinical studies provided evidence of preferential conversion of inactive 5 '- DFUR to active 5 - FU in solid tumours due to the relative overexpression of the final anabolising enzyme , thymidine phosphorylase ( TP ) , in neoplastic tissues more than in normal counterparts . - pubmed
AIM : To establish the differential scanning calorimetric ( DSC ) methodology for controlling the crystal - type B form of nateglinide . - pubmed
METHODS : Accurately weighed pure dried ( P2O5 as desiccant for 4 h at 80 degrees C in vacuum ) fine powder of crystal - type B and H of nateglinide were measured dQ / dT by DSC at heating rate of 10 degrees C. min-1 and temperature between 100 degrees C and 200 degrees C to calculate the enthalpy delta HB and delta HH . - pubmed
Pharmacokinetic study of cisplatin and infusional etoposide phosphate in advanced breast cancer with correlation of response to topoisomerase IIalpha expression . - pubmed
This study evaluated the combination of cisplatin with a prolonged infusion of etoposide phosphate ( EP ) in advanced breast cancer and correlated response to topoisomerase II expression . - pubmed
The protein solution was mixed with trichloroacetic acid ( TCA ) , perchloric acid ( PCA ) or guanidine - HCl ( GHA ) , and then exposed in distilled water . - pubmed
The protein was directly exposed to the solution containing TCA , PCA , GHA , HCl , H ( 2 ) SO ( 4 ) or acetate buffer ( Post - cast method ) . - pubmed
All drugs that directly or indirectly increase central serotonin neurotransmission at postsynaptic 5 - HT ( 1A ) and 5 - HT ( 2A ) receptors can produce SS . - pubmed
It is likely that the activation of 5 - HT ( 1A ) receptors by mirtazapine , the combined serotonin reuptake inhibition by venlafaxine and tramadol , as well as possible serotonin release by tramadol , contributed to the development of SS in this case . - pubmed
The crystal structure of the title compound , C ( 28 ) H ( 26 ) , in the monoclinic space group C2 / c has a columnar packing arrangement with a 7.20 A axis , a feature common to several tetraphenylmethanes . - pubmed
Lansoprazole is extensively metabolized by CYP2C19 and CYP3A4 in the liver , whereas rabeprazole is primarily converted non - enzymatically to rabeprazole - thioether , with only some being oxidized by CYP2C19 and CYP3A4 . - pubmed
This article reviews the pharmacokinetic differences between enantiomers of lansoprazole and rabeprazole in relation to the CYP2C19 genotypes . - pubmed
CYP2C19 influenced the disposition of ( S )- lansoprazole to a greater extent than the ( R)-enantiomer . - pubmed
The R / S ratios for the AUC of lansoprazole in CYP2C19 homEMs , hetEMs and PMs was 12.7 , 8.5 and 5.8 , respectively . - pubmed
On the other hand , ( R )- rabeprazole disposition was influenced to a greater degree by CYP2C19 genetic polymorphisms than ( S )- rabeprazole . - pubmed
However , the R / S ratios for the AUC of rabeprazole in CYP2C19 homEMs , hetEMs and PMs was only 1.8 , 2.2 and 2.4 , respectively , suggesting a lesser effect of CYP2C19 polymorphisms on the stereoselective disposition of rabeprazole compared to lansoprazole . - pubmed
Such a difference in the AUC between rabeprazole enantiomers is likely to be dependent on stereoselectivity in the CYP3A4 - mediated metabolic conversion from rabeprazole - thioether to rabeprazole . - pubmed
We have analyzed the expression of Fn14 and its ligand TWEAK in carotid atherosclerotic plaques and its potential modulation by atorvastatin in vivo . - pubmed
Geranylgeranyl transferase inhibitor , toxin B ( Rac and Rho inhibitor ) , C3 exoenzyme ( Rho inhibitor ) , and Y-27632 ( Rho kinase inhibitor ) also decreased Fn14 expression , implicating the Rho / Rho kinase pathway in the regulation of Fn14 expression . - pubmed
Finally , atorvastatin treatment reduced Fn14 expression in vivo . - pubmed
Atorvastatin diminishes Fn14 expression in vitro and in vivo providing novel information of the beneficial properties of statins . - pubmed
Precise mapping of the putative origin suggested that the replication initiated from a fixed site close to the AT - rich region and proceeded unidirectionally toward the downstream rep201 gene , which was further confirmed by electron microscopic analysis of the ClaI - digested replication intermediates . - pubmed
OBJECTIVE : To evaluate the effect of verapamil on the proliferation of normal gingival fibroblast ( NGF ) in vitro . - pubmed
The 5th passage of NGF was incubated with 0 , 0.1 , 1 , 10 , 100 micromol / L verapamil respectively . - pubmed
An increased whole body calcium uptake partially compensated dietary calcium deficiency and was associated with increased gill chloride cell number in lamellae and filaments in parallel with increased gill Na (+) K (+)- ATPase activity . - pubmed
In addition , a single injection of piscine PTHrP(1 - 34 ) significantly increased whole body calcium uptake and decreased whole body calcium efflux . - pubmed
Administration of PTHrP significantly increased circulating plasma calcium 4 - 24 h postinjection . - pubmed
Genome - wide expression profiles of pog1Delta and other mtDNA - less cells reveal that many genes involved in response to stimulus , energy derivation by oxidation of organic compounds , cellular carbohydrate metabolism , and energy reserve metabolism are induced . - pubmed
DEAD - box proteins catalyze ATP - driven , local structural changes in RNA or RNA - protein complexes ( RNP ) during which only few RNA base pairs are separated . - pubmed
The usefulness of the algorithm is demonstrated for the Muller - Brown potential , amide hydrolysis , and an 89 atom cluster taken from the active site of 4 - oxalocrotonate tautomerase for the reaction which catalyzes 2 - oxo-4 - hexenedioate to the intermediate 2 - hydroxy-2,4 - hexadienedioate . - pubmed
OAF was generated and distributed in a bioaerosol test chamber by delivery of monoterpenes into ozonated air ( 0.1 ppm ) at concentrations of 2.0 ( high ) , 0.75 ( medium ) , or 0.3 ( low ) mgm(-3 ) h(-1 ) . - pubmed
When exposed to OAF ( high and medium concentrations in 0.1 ppm ozone ) there were significant differences after 20 min . - pubmed
Female astrocytes sustain less cell death from oxygen - glucose deprivation ( OGD ) than male astrocytes . - pubmed
Arimidex , an aromatase inhibitor , abolishes these sex differences . - pubmed
Midazolam was administered at 0.3 mg kg(-1 ) IV as pre - medication at least 1 minute prior to induction . - pubmed
The amide I vibrational mode , primarily associated with peptide - bond carbonyl stretches , has long been used to probe the structures and dynamics of peptides and proteins by infrared ( IR ) spectroscopy . - pubmed
MATERIAL AND METHODS : Systemic 3 - NP ( 10 mg / kg ) administration for 14 days significantly reduced locomotor activity , body weight , grip strength , oxidative defense ( raised levels of lipid peroxidation , nitrite concentration , depletion of antioxidant enzyme ) , and mitochondrial enzymes activity in striatum , cortex , and hippocampal regions of the brain . - pubmed
Here , we establish a high - resolution contact map of RutR carP1 complexes for backbone and base - specific contacts , analyze DNA bending , determine the DNA sequence specificity of RutR binding by saturation mutagenesis , demonstrate that uracil but not thymine is the physiologically relevant ligand that inhibits the DNA binding capacity of RutR and build a model of the RutR operator DNA complex based on the crystal structures of RutR and of the DNA - bound family member QacR. - pubmed
Changes in left ventricular mass index ( LVMI ) measured by cardiac ultrasonic indices , HRT parameters , including the original heart rate ( TO ) and slope coeffificient ( TS ) , systolic and diastolic blood pressures ( SBP and DBP ) , as well as blood cholesterol level ( TC ) were measured before and after treatment . - pubmed
Quartz crystal microbalance - based biosensor for the detection of alpha - thalassemia 1 ( SEA deletion ) . - pubmed
Here , a DNA piezoelectric biosensor based on a quartz crystal microbalance ( QCM ) was created to identify abnormal genes causing alpha - thalassemia 1 ( SEA deletion ) . - pubmed
METHODS : The functionalized gold electrode of the quartz crystal was coated with avidin and the biotinylated DNA probe was attached . - pubmed
We have demonstrated that cyclodextrin ( CD ) ( a non - polymeric system ) can be electrospun into nanofibers by itself ; methyl - beta - cyclodextrin ( MbetaCD ) nanofibers were electrospun and it was observed that the success of the electrospinning of the CD nanofibers strongly depends on ( i ) type of solvent , ( ii ) CD solution concentration and ( iii ) intermolecular interactions between the CD molecules . - pubmed
There is also an optimism that new approaches , including response prediction based on sequential 18FDG - PET scanning following induction chemotherapy , and novel drugs targeted at EGF , EGFR , VEGF , and tyrosine kinase inhibition may improve treatment pathways and outcomes . - pubmed
Dioxins exert their toxic actions by acting on phase I and phase II enzymes , such as cytochromes P450 , superoxide dismutase , and glutathione peroxidase , promoting cell proliferation , growth arrest , and apoptosis , affecting cancer homeostasis and neuronal function . - pubmed
Furthermore , the amplitude of postsynaptic G protein - coupled inwardly rectifying K (+ ) channel ( GIRK ) currents elicited by UK-14304 was significantly larger in the diabetic group than in the control group . - pubmed
In addition , the regulation of budR transcription was elucidated and found to be subject to negative control by BudR itself and to positive control by external stimuli such as N -( 3 - oxohexanoyl )- L - homoserine lactone ( OHHL ) quorum sensing signaling molecules and acetate . - pubmed
Interestingly , however , we observed that induction of budR transcription by OHHL or acetate did not require BudR , indicating the involvement of additional regulatory factors in relaying these environmental signals to the budR promoter . - pubmed
Zinc chelation with hydroxamate in histone deacetylases modulated by water access to the linker binding channel . - pubmed
Using Born - Oppenheimer ab initio QM / MM MD simulations , a state - of - the - art approach to simulating metallo - enzymes , we have found that the hydroxamic acid remains to be protonated upon its binding to HDAC8 , and thus disproved the mechanistic hypothesis that the distinct zinc - hydroxamate chelation modes between two HDAC subclasses come from different protonation states of the hydroxamic acid . - pubmed
Instead , our simulations suggest a novel mechanism in which the chelation mode of hydroxamate with the zinc ion in HDACs is modulated by water access to the linker binding channel . - pubmed
This new insight into the interplay between the linker binding and the zinc chelation emphasizes its importance and gives guidance regarding linker design for the development of new class - IIa - specific HDAC inhibitors . - pubmed
[ Successful treatment of post circumcision glanular ischemia - necrosis with hyperbaric oxygen and intravenous pentoxifylline ] . - pubmed
Univariate and multivariate analyses showed that intravenous sufentanil significantly reduced BRF survival rate , hazard ratio 7.78 [ 95 % confidence interval ( CI ) 5.79 , 9.78 ) , for extracapsular extension stage pT 2 or less , hazard ratio 0.44 ( 95 % CI 0.12 , 0.75 ) , Gleason score at least 7 , hazard ratio 1.96 ( 95 % CI 1.65 , 2.26 ) , positive margin , hazard ratio 1.87 ( 95 % CI 1.58 , 2.02 ) and lymph node involvement , hazard ratio 1.77 ( 95 % CI 1.27 , 2.27 , P & gt ; 0.05 ) . - pubmed
CONCLUSION : This retrospective analysis suggests that intraoperative sufentanil administration is associated with an increased risk of cancer relapse after RRP , whereas epidural analgesia , with local anaesthetic and opioid , was not associated with a significant effect . - pubmed
The signalling enzymes PI3 K , TorC2 , PLA2 and sGC are not required for Ras activation and chemotaxis to folate or to steep gradients of cAMP , but they provide a memory of direction and improved orientation of the cell , which together increase the sensitivity about 150 - fold for chemotaxis in shallow cAMP gradients . - pubmed
The presence of short - lived radionuclides in the early solar system , especially ( 60 ) Fe , ( 26)Al , and ( 41 ) Ca , requires a nearby supernova shortly before our solar system was formed , suggesting that the Sun was formed in a massive star - forming region similar to Orion or Carina . - pubmed
Because the Sun formed in a region where many other stars were forming more or less contemporaneously , the bi - polar outflows from all such stars enriched the local region in interstellar silicate and oxide dust . - pubmed
Surrogate markers of myocardial ( troponin T or I and CK - MB ) and renal ( serum creatinine ) injury for primary outcomes were abstracted . - pubmed
The original pyrogallol ( 1,2,3 - trihydroxybenzene ) method , which was developed specifically for superoxide dismutase , is now widely used for measuring superoxide - scavenging of other antioxidants . - pubmed
Post - digestion 18O exchange / labeling for quantitative shotgun proteomics of membrane proteins . - pubmed
Post - digestion ( 18 ) O exchange is a simple and efficient method for differential ( 18 ) O /( 16 ) O stable isotope labeling of two biologically distinct specimens , allowing relative quantitation of proteins in complex mixtures when coupled with shotgun MS - based proteomics . - pubmed
Whilst this protocol depicts solubilization of detergent - insoluble membrane proteins coupled with post - digestion ( 18 ) O labeling , it is amenable to any complex membrane protein mixture . - pubmed
Described approach relies on solubilization and tryptic digestion of membrane proteins in a buffer containing 60 % ( v / v ) methanol followed by differential ( 18 ) O /( 16 ) O labeling of protein digests in 20 % ( v / v ) methanol buffer . - pubmed
The use of methanol - based buffers in the context of the post - digestion ( 18 ) O exchange / labeling eliminates the need for detergents or chaotropes that interfere with LC separations and peptide ionization . - pubmed
, 2012 ) and suggests a possible role for Chk1 Ser317 phosphorylation as a biological marker for future analyses of complex mixtures of PAHs . - pubmed
Prognostic impact of ( 18 ) FDG - PET - CT findings in clinical stage III and IIB breast cancer . - pubmed
RESULTS : With increasing decoction time , the acute toxicity of detoxicated Fuzi became decreased in the following order : dBfp-30 ( LD50 of 145.1g / kg ; MTD of 70g / kg ; MLD of 100g / kg ; NOAEL of 70g / kg ) & gt;dBfp-60 ( too large LD50 ; MTD of 160g / kg ; MLD of 190g / kg ; NOAEL of 100g / kg ) & gt;dBfp-120 ( no LD50 ; unlimited MTD ; unlimited MLD ; NOAEL of 130g / kg ) . - pubmed
Glucose , insulin , appetite regulatory hormones , GLP-1 , GLP-2 , appetite sensations , and markers of inflammation were measured at a following standardised breakfast , that is at 11 to 14 h post the evening meals . - pubmed
TCBQ reductase ( PcpD ) , an FMN- and NADH - dependent reductase , catalyzes the reduction of TCBQ to tetrachlorohydroquinone . - pubmed
In the presence of PcpD , TCBQ formed by pentachlorophenol hydroxylase ( PcpB ) is sequestered until it is reduced to the less toxic tetrachlorohydroquinone , protecting the bacterium from the toxic effects of TCBQ and maintaining flux through the pathway . - pubmed
The toxicity of TCBQ may have exerted selective pressure to maintain slow turnover of PcpB ( 0.02 s(-1 ) ) so that a transient interaction between PcpB and PcpD can occur before TCBQ is released from the active site of PcpB. - pubmed
Recent progress has begun to reveal the often complex and changing roles of phosphotyrosine and phosphoinositide phosphatases in regulation of immunoreceptor signaling . - pubmed
Quantitative assessment of global lung inflammation following radiation therapy using FDG PET / CT : a pilot study . - pubmed
The aim of this study was to quantify global lung inflammation following radiation therapy using FDG PET / CT . - pubmed
Minocycline modulates cytokine and gene expression profiles in the brain after whole - body exposure to radiation . - pubmed
Minocycline treatment significantly increased the levels of interleukin ( IL )- 10 , IL-15 and vascular endothelial growth factor ( VEGF ) in the brain on day 4 in one or more irradiated groups compared to radiation - alone ( p<0.05 ) . - pubmed
Microarray analysis of brains on day 32 showed that while radiation increased expression of inflammatory genes such as Il1f10 , Il17 , Tnfrsf11b , Tnfsf12 , Il12b and Il1f8 , these were no longer up - regulated in the minocycline - treated groups . - pubmed
Pathway analysis based on gene data suggested that catenin - beta1 and tumor suppressor - related transcription regulation were significantly activated by radiation and/or minocycline ( activation z - score & gt;2.0 ) . - pubmed
The addition of the outer membrane phospholipase gene pldA on a multicopy plasmid in a vpsC or vacJ mutant restored its resistance to SDS , suggesting a restoration of lipid asymmetry to the outer membrane . - pubmed
Increased PldA levels also failed to restore the mutant 's phospholipid profile to that of the wild type . - pubmed
To determine if treatment with ACTH , a drug widely used to treat early - life seizures , altered outcome we administered ACTH once per day subcutaneously during the time of the induced interictal spike activity . - pubmed
We demonstrate an increase in the abundance of PHEOPHORBIDE a OXYGENASE ( PAO ) , a key enzyme of Chl breakdown , together with an accumulation of phyllobilins , that is , products of PAO - dependent Chl breakdown , in both species . - pubmed
The roles of the PAO / phyllobilin pathway during different plant developmental processes that involve Chl breakdown , such as leaf senescence and desiccation , fruit ripening and seed maturation , are discussed . - pubmed
Sulfonamides and their isosters as carbonic anhydrase inhibitors . - pubmed
Molecules containing the sulfonamide group ( R - SO2NH2 ) as well as its structurally related isosters , sulfamido ( R - NH - SO2NH2 ) and sulfamato ( R - O - SO2NH2 ) , constitute the most important class of inhibitors acting on the metalloenzyme carbonic anhydrase ( EC 4.2.1.1 ) . - pubmed
The current review is intended to highlight the structural basis of the interactions of sulfonamide - like groups within the active site of the carbonic anhydrases and will summarize the clinical use of the most interesting molecules for the treatment of relevant pathologies , such as glaucoma , obesity , cancer and CNS - affecting diseases . - pubmed
They survived and retained dopaminergic function following stereotaxic injection into the MPTP - lesioned right - side substantia nigra as indicated by SPECT measurement of DAT activity . - pubmed
Injected cells preserved and supplemented the remaining endogenous population of dopamine neurons ( TH - positive cell ipsilateral / contralateral ratio was 56.81 % + - 7.28 % vs. 3.86%+-1.22 % in vehicle - injected controls ; p<0.05 ) . - pubmed
EKB-569 - treated infected gerbils had significantly decreased abundance of Cox-2 , Adam17 and Egfr gastric transcripts relative to infected animals on control diet . - pubmed
Serum total testosterone , free testosterone , interleukin 1 ( IL ) alpha and beta , IL6 , tumor necrosis factor ( TNF ) alpha , orexin , galanin , neuropeptide Y , tumor necrosis factor - like weak inducer of apoptosis and tumor necrosis factor receptor - associated factor 6 , and C - reactive protein ( CRP ) levels were investigated . - pubmed
In addition to cachexia , serum CRP , testosterone , and TNF alpha levels were statistically significantly correlated with OS in refractory cachexia . - pubmed
This end - stage term of cancer duration may be predicted by decreasing serum testosterone and increasing serum TNF alpha levels , as well as serum CRP levels . - pubmed
Comparison of single intra operative versus an intra operative and two post operative injections of the triamcinolone after wedge excision of keloids of helix . - pubmed
OBJECTIVE : To compare single intra - operative versus an intra - operative and two post - operative injections of triamcinolone after wedge excision of keloids of helix . - pubmed
The performance of DFT methodology to predict with accuracy the isotropic hyperfine coupling constants ( hfccs ) of aromatic radicals containing ( 14 ) N nucleus is investigated by an extensive study in which 165 hfccs , belonging to 38 radical species , are obtained from calculations with B3LYP and PBE0 functionals combined with 6 - 31 G * , N07D , TZVP , and EPR - III basis sets , and are compared to the reported experimental data . - pubmed
The contribution of K (+ ) channels , sGC / cGMP pathway , phosphodiesterases , and extra and intracellular Ca ( 2 + ) sources were analyzed . - pubmed
In the control group , TERPY relaxation was induced by the activation of K (+ ) channels and reduction of the calcium influx , while in the OVA group , these same effects were also brought about by TERPY , but with participation of the sGC / cGMP pathway . - pubmed
In both groups , SNP - induced relaxation occurred through the activation of K (+ ) channels , sGC / cGMP pathway and reduction of calcium influx . - pubmed
RESULTS : The mean size of the liposomal DHA was 137 + - 12 nm with a slightly negative charge ; the encapsulation efficiency of DHA in the liposomes as determined by LC - MS / MS ranged from 60 to 80 % ; our formulation is resistant to oxidation and stable over a range of pH ( 1.0 - 7.4 ) at 37 C for a duration of two hours . - pubmed
It altered proteins involved in cell growth , cell cycle , and apoptosis more effectively than free DHA in both cell lines ; it up - regulated p21 and cleaved PARP , while P - AKT and P - S6 were down - regulated . - pubmed
Lithocholic acid ( LCA ) , a hepatotoxic and carcinogenic bile acid , is metabolized to 3 - ketocholanoic acid ( 3 - KCA ) by cytochrome P450 3A ( CYP3A ) . - pubmed
Chromatographic separation was achieved on a Waters ACQUITY UPLC C18 column ( 50x2.1 mm , 1.7mum ) with a gradient system consisting of 0.1 % v / v formic acid in water ( solvent A ) and 0.1 % v / v formic acid in acetonitrile ( solvent B ) . - pubmed
Enzyme kinetics experiment indicated that LCA 3 - oxidation followed the Michaelis - Menten model with an apparent Km of 26 + -7muM and Vmax of 303 + -50pmol / min / mg protein . - pubmed
This novel UPLC - MS / MS method for quantifying 3 - KCA offers a specific , sensitive , and fast approach to determine liver microsomal LCA 3 - oxidation . - pubmed
INTRODUCTION : Bupivacaine is the most commonly used local anaesthetic for spinal anaesthesia ( SA ) . - pubmed
Transcriptomics and methylomics of CD4 - positive T cells in arsenic - exposed women . - pubmed
We therefore analyzed the transcriptomes ( by RNA sequencing ) and methylomes ( by target - enrichment next - generation sequencing ) of primary CD4 - positive T cells from matched groups of four women each in the Argentinean Andes , with fivefold differences in urinary arsenic concentrations ( median concentrations of urinary arsenic in the lower- and high - arsenic groups : 65 and 276 mug / l , respectively ) . - pubmed
Key genes regulating the immune system , such as tumor necrosis factor alpha and interferon gamma , as well as genes related to the NF - kappa - beta complex , were significantly downregulated in the high - arsenic group . - pubmed
Differentially methylated regions that were hyper - methylated in the high - arsenic group showed enrichment for immune - related gene ontologies that constitute the basic functions of CD4 - positive T cells , such as isotype switching and lymphocyte activation and differentiation . - pubmed
In conclusion , chronic arsenic exposure from drinking water was related to changes in the transcriptome and methylome of CD4 - positive T cells , both genome wide and in specific genes , supporting the hypothesis that arsenic causes immunotoxicity by interfering with gene expression and regulation . - pubmed
By screening subclones from each of the epigenetically heterogeneous transfectant clones , we finally obtained three optimal subclones that expressed luciferase and EGFP after 5 - aza - dC treatment with high signal - to - noise ratios . - pubmed
They share homology to type B glycosyltransferases and TagB , an enzyme catalyzing glycerol transfer to an N - acetylmannosamine residue in teichoic acid biosynthesis . - pubmed
Analysis of a cellular pool of nucleotide - activated sugars confirmed the presence of the CMP - activated nonulosonic acid derivatives , which are most likely serving as substrates for the corresponding transferase . - pubmed
Single gene knock - out mutants targeted at either transferase were analyzed for S - layer O - glycan composition by ESI - MS , confirming the loss of the nonulosonic acid derivative . - pubmed
Ambroxol treatment did not affect expression of SP - B and SP - C. - pubmed
Post carbon removal nitrifying MBBR operation at high loading and exposure to starvation conditions . - pubmed
This study investigates the performance of MBBR nitrifying biofilm post carbon removal at high loading and starvation conditions . - pubmed
coli as well as pepsin , bovine serum albumin and lysozyme were the analytes studied in contact with the polymer nanolayers in phosphate buffer solution , pH 7.2 . - pubmed
In contact with polyaramides , the proteins of pI & gt ; 4 reversibly formed the 0.2 - 2.5 nm - thick adsorption layers decomposing on further rinsing with the protein - free eluent . - pubmed
In contact with PANI , the proteins formed stable adsorption layers at pH 7.2 , which needed the pH 3.0 to be eluted . - pubmed
Thus , in a neutral aqueous medium optimal for separation of biopolymers , polyaramides , although did not retain DNA , had a weaker affinity to proteins as compared to PANI . - pubmed
Background & amp ; Objective : Catalase ( CAT ) is an important endogenous antioxidant enzyme that detoxifies H2O2 into water and oxygen , consequently limiting the deleterious effects of reactive oxygen species . - pubmed
The products show two - step dehydration behaviour from thermogravimetric analysis ( TGA ) , and a higher degree of purity and homogeniety than commercial zinc phosphate pigment . - pubmed
We report the vibronic structure of the X+ 2A '' ( D0 ) , A+ 2A ' ( D1 ) and B+ 2A '' ( D2 ) electronic states of the 9H adenine cation , from the adiabatic ionization energy ( AIE ) up to AIE + 1.8 eV. - pubmed
CYP2C9 and VKORC1 polymorphisms are essential determinants in the genotype - guided dosing of warfarin and may distinguish patients who would benefit from switching to direct oral anticoagulants ( DOACs ) . - pubmed
RESULTS : We found that treatment of all HNSCC cells with Gefitinib and Erlotinib , two Food Drug Administration - approved EGFR inhibitors , blocked the activity of Akt / mammalian target of the rapamycin ( mTOR ) and extracellular signal - regulated kinase , two crucial downstream effectors of EGFR , but up - regulated IKKbeta / NF - kappaB signalling . - pubmed
In keeping with these , IKKbeta inhibitor CmpdA synergistically enhanced the efficacy of EGFR inhibitors to further inhibit in vitro HNSCC cell growth . - pubmed
CONCLUSION : Our data demonstrated that co - targeting EGFR and IKKbeta with Gefitinib and IKKbeta inhibitors could provide a potential novel therapy for head and neck squamous cell cancer . - pubmed
Certain anti - polymerization agents such as colchicine appear to down - regulate most immune cell types , while inducing dendritic cell maturation and increasing M1 macrophage population . - pubmed
After 2 h stimuli of 10 - 7 M dexamethasone ( DX ) , the osteogenic genes ' expressions were detected by real - time polymerase chain reaction ( RT - PCR ) at Zeitgeber times 0 , 4 , 8 , 12 , 16 , 20 , and 24 . - pubmed
Induced by DX , the mRNA expression of Col-1 , OPN , and IBSP within the loaded / unloaded rPDLCs oscillated as that of the main clock gene Per-1 . - pubmed
Stimulation of natural killer cells with a CD137 - specific antibody enhances trastuzumab efficacy in xenotransplant models of breast cancer . - pubmed
Overcoming the materials integration challenge for complex oxides on silicon using virtual substrates opens a path to develop new electronic devices in the More than Moore era and silicon integrated quantum computation hardware . - pubmed
OBJECTIVE : To assess the therapeutic effect of a novel tension band using 3.0 mm cannulated screw combined with a titanium cable and specific shims comparatively with patellotibial tubercle cerclage in comminuted fractures of the inferior pole of the patella . - pubmed
In this study , experiments were conducted to investigate the mode of occurrence and the leaching behavior of valuable trace metals ( U , V , and Ga ) from CA and ( NH4 ) 2SO4 - treated CA ( NCA ) , based on the recovery of aluminum . - pubmed
On the other side , a higher liquidus temperature , contributing to the dissolutions of Al3 + and Ca2 + , could promote the leaching of U from NCA as well , whereas the water - leaching behaviors of V and Ga involved a sophisticated trend with temperature > 40C. - pubmed
RESULTS : Based on the neutral theory of molecular evolution , we propose a methylation - driven model ( MDM ) that allows predicting the changes in frequencies / proportions of the 16 dinucleotides and in the GC content of a genome given the known number of CpG to TpG / CpA mutations . - pubmed
The application of MDM to the 10 published vertebrate genomes shows that , for most of the 16 dinucleotides and the GC content , a good consistency is achieved between the predicted and observed trends of changes in the frequencies and content relative to the assumed initial values , and that the model performs better on the mammalian genomes than it does on the lower - vertebrate genomes . - pubmed
In the presence of target protein lysozyme , the interaction of lysozyme with aptamer led to the dissociation of dsDNA and release of the aptamer - lysozyme complex to solution , with DNA - c retaining on the electrode ; then , the photosensitizer phloxine B ( PB ) was assembled on the electrode since GO can simultaneously adsorb DNA - c and PB molecules . - pubmed
Upon irradiation by a green LED , 1O2 was generated by photocatalysis of PB molecules and then cleaved the DNA - c , leading to remarkably decreased impedance signals that linearly respond with the logarithm of lysozyme concentration . - pubmed
Graphical abstract An on - line assembly of photosensitizers and DNA on electrode was developed using graphene oxide a cartridge and the photocatalytic electrosensor can be used for label - free detection of lysozyme as low as 1 pM. - pubmed
Similarly to Levetiracetam ( LEV ) , BRV works by binding SV2A vesicles with a high affinity and a linear pharmacokinetic profile . - pubmed
The Cd concentrations in the above - ground portion of the plants were significantly decreased as a result of the incorporation of all biochars compared to the unamended soil , with reduction ratios following the order PM ( 78 % ) > > WS ( 31 % ) RH ( 29 % ) MB ( 28 % ) CY ( 26 % ) > CE ( 19 % ) . - pubmed
Finally , ubiquitination of proliferating cell nuclear antigen ( PCNA ) is induced during CCl4 - induced liver fibrosis and associated with the DNA damage response ( DDR ) . - pubmed
While mRNA levels remained unaffected by AgNO3 or cit - AgNP , the enzyme activity of GPx was inhibited by AgNO3 ( 1 microM ) and cit - AgNP ( 5 microM ) and TrxR activity was inhibited by AgNO3 ( 0.4 microM ) and cit - AgNP ( 1 , 5 microM ) . - pubmed
The comparison of immunoglobulin levels with HLA antigens , was less striking , although HLA - A2 appeared to be associated with low levels of IgE. - pubmed
Effect upon serum LH , FSH , estradiol and progesterone . - pubmed
Interaction of alloxan and anomers of D - glucose on glucose - induced insulin secretion and biosynthesis in vitro . - pubmed
Prior exposure to alloxan alone produced marked inhibition of subsequent glucose - induced insulin secretion and biosynthesis . - pubmed
The anomeric preference of D - glucose for protecting islet cells from the inhibitory effect of alloxan on glucose - induced insulin secretion and biosynthesis was similar to that for triggering insulin secretion . - pubmed
It is suggested that a glucoreceptor , stereospecific to the alpha anomer of D - glucose , may exist for both insulin secretion and biosynthesis . - pubmed
The HO mRNA level increased to a maximum 15 h after heme injection . - pubmed
In contrast , expression of HO was maximal about 45 h after heme injection . - pubmed
Effects of the preferential dopamine autoreceptor antagonist ( + ) - AJ76 in the intracranial self - stimulation paradigm . - pubmed
If metastatic tumor cells have decreased levels of active homotypic cell adhesion molecules ( CAMs ) , this might aid their escape from the primary tumor . - pubmed
Dissection of helix capping in T4 lysozyme by structural and thermodynamic analysis of six amino acid substitutions at Thr 59 . - pubmed
Threonine 59 , a helix - capping residue at the amino terminus of the longest helix in T4 phage lysozyme , was substituted with valine , alanine , glycine , serine , asparagine , and aspartic acid . - pubmed
The crystal structures of the different variants showed that changes in conformation occurred at the site of substitution , including Asp 61 , which is nearby , as well as displacement of a solvent molecule that is hydrogen - bonded to the gamma - oxygen of Thr 59 in wild - type lysozyme . - pubmed
This suggests a structural basis for the heretofore puzzling observations that asparagine is more frequently observed as a helix - capping residue than threonine [ Richardson , J. S. , & amp ; Richardson , D. C. ( 1988 ) Science 240 , 1648 - 1652 ] yet Thr ---- Asn replacements at N - cap positions in barnase were found to be destabilizing [ Serrano , L. , & amp ; Fersht , A. R. ( 1989 ) Nature 342 , 296 - 299]. - pubmed
The duration of this hyperalgesia was prolonged by the phosphodiesterase inhibitors , isobutylmethylxanthine and rolipram . - pubmed
Forskolin hyperalgesia was antagonized by the Rp isomer of cyclic adenosine-3'5 '- monophosphothioate , an analog of cAMP that prevents the phosphorylation of the cAMP protein kinase . - pubmed
Although the cAMP second messenger system contributes to primary afferent hyperalgesia , we found no evidence for a contribution of protein kinase C. - pubmed
Thus , hyperalgesia induced by prostaglandin E2 , prostacyclin ( prostaglandin I2 ) , ( 8R,15S )- dihydroxyicosa ( 5E-9,11,13Z ) tetraenoic acid , the adenosine A2 - agonist 2 - phenylaminoadenosine , 8 - bromocyclic adenosine monophosphate and the direct activator of adenyl cyclase , forskolin , were not significantly attenuated by the selective inhibition of protein kinase C by the 19 - 31 fragment of protein kinase C. - pubmed
Two other inhibitors of protein kinase C , sphingosine and staurosporine , also failed to attenuate prostaglandin E2 - induced hyperalgesia . - pubmed
The three thrombolytic agents currently available for the management of AMI are streptokinase , alteplase , and anistreplase . - pubmed
The amino acid sequence deduced from its coding base sequence resembles that of T cell activating protein , most conspicuously in cysteine residues , 10 out of 11 of which occupy identical positions in an overall sequence homology of 24.8 % . - pubmed
The assay that has been developed using these modifications and a solid - phase second antibody separation step , is both sensitive ( with a lower detection limit of 0.5 pg / tube ) , reliable and simple and has the advantage that methyl oximation of the sample protects the PGE from degrading prior to and during the assay . - pubmed
At the end of the 12 - week treatment period , the mean number of pustules ( + /- SEM ) had decreased from 57.8 ( + /- 8.6 ) to 3.9 ( + /- 1.6 ) in the acitretin group and from 57.1 ( + /- 14.1 ) to 5.7 ( + /- 2.7 ) in the etretinate group . - pubmed
Substance P mimics the increase in vascular permeability caused by vagal nerve stimulation and neurokinin A mimics noncholinergic bronchoconstriction by vagal nerve stimulation . - pubmed
Incubation with micromolar levels of ATP dissociates this complex in vitro into p53 and hsc70 110 - kDa components . - pubmed
Incubation of the immunopurified p53 - dnaK complex with ATP resulted in the dissociation of the p53 - dnaK complex as it did with the p53 - hsc70 complex . - pubmed
Neither inhibition of ethanol metabolism with 4 - methylpyrazole , nor a 10 - fold elevation of acetaldehyde levels by inhibition of aldehyde dehydrogenase with cyanamide or disulfiram , affected the magnitude of the GSH depletion observed in vivo . - pubmed
This finding suggests that antigen presentation by mature M phi is a reversible function regulated by T cell factors . - pubmed
The fractionation of arginine - rich histones from fetal calf thymus by exclusion chromatography . - pubmed
Addition of urate to anaerobic neutrophils causes the reduction of a b - type cytochrome , a likely component of the neutrophil superoxide generating system . - pubmed
Bone resorption as measured by increases in the marrow ( inner ) diameter of the midshaft of the femur and humerus and by the weekly losses of total [ ( 3 ) H ] tetracycline and [ ( 3 ) H]collagen per whole bone was not significantly different among any of the groups studied . - pubmed
Hill coefficients and IC50 values derived from the inhibition of specific [ 3H ] L - quinuclidinyl benzilate receptor binding suggest the presence of two muscarinic binding sites , differing both in affinity for pirenzepine and in tissue distribution . - pubmed
Special equipment for the reaction was made with a uv - irradiation lamp and a reaction coil of Teflon tubing inside a temperature - controlled reaction box . - pubmed
When 18 - hydroxy-11 - deoxycorticosterone was incubated with adrenocortical mitochondria fortified by adding exogenously adrenodoxin reductase and adrenodoxin in the presence of an NADPH - generating system , the steroid was converted to two products which were separated by high performance liquid chromatography . - pubmed
Production of the unidentified substance was also shown when 18 - hydroxy-11 - deoxycorticosterone was incubated with purified cytochrome P-45011 beta and its electron transport system . - pubmed
The enzyme system effecting this conversion is an NADPH - requiring microsomal oxygenase found in the liver . - pubmed
Thus , these two alkaloids appear to be new sodium channel neurotoxins acting through different mechanisms , dibromosceptrin modifying the channel inactivation characteristics and clathrodin probably influencing channel ionic conductance . - pubmed
Differential modulation by cyclothiazide and concanavalin A of desensitization at native alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionic acid- and kainate - preferring glutamate receptors . - pubmed
Concanavalin A , cyclothiazide , and aniracetam , ligands that modulate desensitization at glutamate receptors , were tested for their actions on responses at kainate - preferring receptors in dorsal root ganglion ( DRG ) neurons and at alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionic acid ( AMPA)-preferring receptors in hippocampal neurons . - pubmed
However , in hippocampal neurons concanavalin A produced only weak potentiation of responses to kainate and 5 - chlorowillardiine , and after treatment with lectin responses to 5 - chlorowillardiine remained strongly desensitizing . - pubmed
The different sensitivities of native AMPA- and kainate - preferring glutamate receptors to cyclothiazide and concanavalin A should prove useful for the differentiation of glutamate receptor subtypes in other areas of the central nervous system . - pubmed
In order to achieve isolation of endo - alpha - D - mannosidase , a Golgi - located processing enzyme that accomplishes deglucosylation of glycoproteins with N - linked carbohydrate units by cleaving the linkage between the glucose - substituted mannose residue and the remainder of the oligosaccharide , we have prepared an affinity matrix ( Glc alpha 1 - ->3Man - O -( CH2 ) 8CONH - Affi - Gel 102 ) containing the derivative of the characteristic disaccharide product of this enzyme . - pubmed
This purified enzyme was free of other processing glycosidases and was completely inhibited by Glc alpha 1 - ->3 ( 1 - deoxy ) mannojirimycin . - pubmed
Examination of the endomannosidase by SDS - polyacrylamide gel electrophoresis revealed a doublet ( M(r ) 60,000 and 56,000 ) with the bands being of approximately equal density . - pubmed
Degradation of c - fos protein ( c - FOS ) in the cytoplasm is very rapid in vivo and constitutes a crucial regulation of the nuclear steady - state level through the control of the amount of full - length molecules available for nuclear transport . - pubmed
Using cytoplasmic extracts from various origins , we report herein that c - FOS degradation can be initiated in a calcium - dependent manner which involves cysteine proteases called milli- and micro - calpain . - pubmed
K1 ( Glu-107 - ->Lys ) point mutants of SVLT , which are unable to bind to the Rb protein family or induce neoplastic transformation , are defective for blocking differentiation in the case of SVLT1 - 121 but retain the ability to block differentiation in the case of full - length SVLT . - pubmed
Blood was taken at rest and 0 and 2 min after exercise for measurement of lactate and ammonia . - pubmed
Resting ammonia ( mumol / l ) was higher in NYHA III ( 65.7 ( 6.0 ) ) than in NYHA II ( 48.0 ( 3.7 ) , P = 0.016 ) ; no difference was found between controls ( 48.0 ( 7.1 ) ) and patients . - pubmed
The overall lactate and ammonia response to exercise was greater in NYHA III than in NYHA II and controls ( P & lt ; 0.05 ) . - pubmed
At volitional exhaustion , peak lactate ( mmol / l : NYHA III 3.31 ( 0.26 ) ; NYHA II 2.56 ( 0.16 ) ; controls 2.71 ( 0.22 ) ; P = 0.022 NYHA III v NYHA II ) and ammonia ( mumol / l : NYHA III ) 126.4 ( 8.97 ) ; NYHA II 92.9 ( 7.23 ) ; controls 109 ( 16.3 ) ; P = 0.006 NYHA III v NYHA II ) were higher in severe congestive heart failure . - pubmed
Ribonucleotide reductase ( RNR ) catalyzes the rate limiting step in the de novo synthesis of deoxyribonucleotides by directly reducing ribonucleotides to the corresponding deoxyribonucleotides . - pubmed
To keep balanced pools of deoxyribonucleotides , all nonviral RNRs studied so far are allosterically regulated . - pubmed
The amino acid sequence identities with the mouse R1 and R2 subunits are 58 % and 63 % , respectively . - pubmed
The R2 protein contains an iron - tyrosyl free radical center verified by EPR spectroscopy and iron analyses . - pubmed
Measurement of cytidine 5 '- diphosphate reduction by the trypanosome RNR in the presence of various allosteric effectors showed that the activity is highest with dTTP , dGTP , or dATP and considerably lower with ATP . - pubmed
The presentation of P12 - 25 is sensitive to cytochalasin B and D , brefeldin A and gelonin , a ribosome - inactivating protein synthesis inhibitor , but less sensitive or resistant to lactacystin , a highly specific inhibitor of the proteasome . - pubmed
Interestingly , CA-074 , a selective inhibitor of cathepsin B , inhibited presentation of the polypeptide , indicating its involvement in the degradation of the P12 - 25 polypeptide . - pubmed
Amino acid residues 420 - 434 of the first disulfide loop of the sex hormone binding globulinlike domain of protein S are involved in the interaction of protein S with C4BP . - pubmed
These results suggest that Lys-423 , Gln-427 and Lys-429 of protein S are important for normal binding to C4BP . - pubmed
This suggests that both in the peptide PSP-420 and in protein S , Lys-423 and Lys-429 significantly contribute to binding to antibody LJ-56 . - pubmed
When peptides PSP 420 and SL-6 ( residues 447 - 460 ) with carboxyterminal amide or carboxylate moieties were compared to their ability to inhibit C4BP - protein S complexation , PSP-420 - amide was the most potent . - pubmed
Among them , the bridged sulfactams ( X = OSO3H ) were found to be effective beta - lactamase inhibitors . - pubmed
External guide sequences ( EGSs ) are short oligoribonucleotides , which are designed to bind to a given RNA target and form a precursor tRNA - like complex . - pubmed
COUP - TF factors , which are able to dimerize with the RXRs and to compete with the retinoid receptors for their DNA binding sites , are suspected to modulate the retinoid signal transduction pathway . - pubmed
The aim of this work is to assess whether RA - induced modifications in the expression of Krox-20 and Hox genes correlate with alterations of the expression of COUP - TF genes . - pubmed
In addition to spatial modifications in the expression patterns of Krox-20 and Hox genes , we report here an upregulation of the expression level of COUP - TFI after RA exposure . - pubmed
These data suggest an involvement of COUP - TFI in the generation of RA - induced abnormalities , but do not support the hypothesis of an involvement of this factor in the regulation of the expression of Hox or Krox-20 genes . - pubmed
The administration of GBL increased CRE- and AP-1 DNA - binding activities in the cerebral cortex and thalamus , but not in other regions such as the hippocampus , cerebellum or pons + medulla oblongata , at doses which induced absence seizures . - pubmed
Not only the absence - seizure behavior but also the increased CRE- and AP-1 DNA - binding activities in the thalamocortical regions were reversibly inhibited by ethosuximide , a typical anti - absence drug , and the GABAB antagonists CGP 35348 and CGP 46381 . - pubmed
A gel - supershift assay revealed that the GBL - induced CRE - binding activity was supershifted by an anti - CRE - binding protein ( CREB ) antibody , and that AP-1 DNA - binding activity was blocked by anti - c - Jun and anti - c - Fos antibodies . - pubmed
These results suggest that increased CRE- and AP-1 DNA - binding activities in the cerebral cortex and thalamus are related to the pathogenesis of generalized absence seizures and that these increases in DNA - binding activity are related to ethosuximide- and GABAB antagonist - sensitive abnormal neuronal activity in the thalamocortical circuit . - pubmed
Time - resolved EPR of spin - polarized mobile H atoms in amorphous silica : The involvement of small polarons . - pubmed
OBJECT : The possible role of the polyamine interconversion pathway on edema formation , traumatic injury volume , and tissue polyamine levels after traumatic brain injury ( TBI ) was studied using an inhibitor of the interconversion pathway enzyme , polyamine oxidase . - pubmed
CONCLUSIONS : These results demonstrate , for the first time , that the polyamine interconversion pathway has an important role in the increase of putrescine levels after TBI and that the polyamine oxidase inhibitors , blockers of the interconversion pathway , can be neuroprotective against edema formation and necrotic cavitation after TBI . - pubmed
Three days following MCA occlusion , terminal deoxynucleotidyltransferase - mediated dUTP - biotin nick end labeling ( TUNEL staining ) revealed a distinct pattern of nuclear staining in many neurons around the ischemic core . - pubmed
The levels of calcium , phosphorus and alkaline phosphatase in their sera , as well as their feed intake and body weight , were evaluated weekly . - pubmed
The parallel increase of both marker molecules indicates increased systemic NO production during GH treatment . - pubmed
All kidneys were randomized to receive 3.0 mM supplemental GSH to perfusate or no supplementation ( control ) and were preserved by either cold storage ( CS ) in UW or machine perfused ( MP ) in UW - machine perfusate solution ( MPS ) . - pubmed
An inhibitor of Janus kinase 3:4-(4 - hydroxyphenylamino)-6 , 7 - dimethoxyquinazolin-1 - ium chloride methanol solvate . - pubmed
CH ( 4 ) O ( WHI - P131 , an inhibitor of Janus kinase 3 ) , contains four hydrogen bonds . - pubmed
Cleavage of SNAP-25 by botulinum toxin type A requires receptor - mediated endocytosis , pH - dependent translocation , and zinc . - pubmed
Methylamine hydrochloride , an agent that prevents pH - dependent translocation , also antagonized toxin - induced paralysis and SNAP-25 cleavage . - pubmed
Furthermore , zinc chelation antagonized toxin - induced paralysis and SNAP-25 cleavage . - pubmed
These results demonstrate that cleavage of SNAP-25 by botulinum serotype A fulfills the requirements of the multistep model of botulinum toxin action that includes receptor - mediated endocytosis , pH - dependent translocation , and zinc - dependent proteolysis . - pubmed
The described effects are more pronounced in the E194Q mutant than in the E204Q mutant demonstrating different roles of these two glutamates / glutamic acids at least in the final stages of the catalytic cycle of bacteriorhodopsin . - pubmed
The cloning of the affected genes has now provided novel insights into the role and regulation of signaling by BMP , Nodal , Wnt , FGF , Hedgehog , Delta , Slit , retinoic acid and lipids . - pubmed
Inductively coupled plasma mass spectrometry ( ICP / MS ) with direct injection nebulization ( DIN ) was used to evaluate novel impinger solution compositions capable of capturing elemental mercury ( Hg0 ) in EPA Method 5 type sampling . - pubmed
An iodine based impinger solution proved to be very efficient for Hg0 capture and was amenable to direct analysis by DIN - ICP / MS . - pubmed
Using DIN - ICP / MS , it was demonstrated for the first time that iodine can be generated just prior to impinger sampling for efficiently oxidizing Hg0 and retaining it in solution as HgI4 ( 2 - ) . - pubmed
Due to the increased interest in Hg speciation from combustion sources and the potential for using DIN - ICP / MS for multiple metals analyses , an impinger sampling train for gaseous Hg speciation and multiple metals analyses using DIN - ICP / MS analyses is presented . - pubmed
The DIN - ICP / MS instrumental detection limit was 0.003 ppb , and MDLs ranged from 0.007 to 0.116 microg / L ( ppb ) in a variety of impinger solutions used for Hg capture . - pubmed
Substitution of Phe by leucine in a ZFY peptide permits Zn ( 2 +)-dependent folding . - pubmed
In the present study , we examined which features of the flavonoid structures were associated with induction of UGT1A1 and whether common drug - metabolizing enzyme inducers also produce this induction . - pubmed
We also determined whether flavonoid treatment affected sulfate conjugation and CYP1A1 activity . - pubmed
In contrast , all tested 5,7 - dihydroxyflavones and -flavonols induced CYP1A1 activity ( ethoxyresorufin deethylation ) . - pubmed
Together , these results strongly suggest that the flavonoid induction of UGT1A1 is through a novel nonaryl hydrocarbon receptor - mediated mechanism . - pubmed
Therefore , we can conclude that long - lived DA receptor sensitization , produced by repeated DA D2 agonist treatments in ontogeny , is associated with enhanced AMPH - induced DA release in the neostriatum in adulthood , but is not accompanied by evident alteration in quinpirole - induced DA release . - pubmed
Nitric oxide , vasoactive intestinal polypeptide , and/or neuropeptide Y are co - localised with cholinesterase and/or acetylcholine transporter in some of the nerve fibres supplying the prostate . - pubmed
A dense network of cholinesterase - staining fibres supplies both prostate epithelium and stroma , suggesting a role of acetylcholine and/or co - localised neuropeptides in the modulation of prostatic secretions , as well as smooth muscle tone . - pubmed
A predominantly epithelial location for prostate muscarinic receptors indicated a major secretomotor role for acetylcholine . - pubmed
He underwent total gastrectomy with splenectomy , as well as D3 dissection , and received postoperative chemotherapy combining oral uracil and futrafur ( UFT ) with cisplatin ( CDDP ) , but results showed recurrence of multiple abdominal lymph node metastases around the aorta . - pubmed
Electrophysiological ( resting membrane potential , V(m ) ) and biochemical ( neuron - specific enolase activity [ NSE ] ) measurements were taken every 2 d for a period of 16 d. A voltage - sensitive oxonol dye together with flow cytometry was used to measure relative changes in V(m ) . - pubmed
Neuron - specific enolase activity was measured by following the production of phosphoenolpyruvate from 2 - phospho - d - glycerate at 240 nm . - pubmed
The HFE mutation is common and , when homozygous , can lead to a morbid accumulation of body iron and the disease hereditary hemochromatosis . - pubmed
Dietary iron content was hypothesized to interact with the HFE genotype to influence oxidative damage in mammary and colon tissue . - pubmed
Neuroprotective effect of ACTH ( 4 - 9 ) in degeneration of hippocampal nerve cells caused by dexamethasone : morphological , immunocytochemical and ultrastructural studies . - pubmed
The purpose of this research was an assessment of the neuroprotective effect of ACTH ( 4 - 9 ) in degenerative changes of hippocampal neurons induced by synthetic GC - dexamethasone . - pubmed
It was shown that dexamethasone administered in toxic doses evokes neuronal death in layer CA3 of the hippocampus . - pubmed
Dexamethasone - induced damage to hippocampal pyramidal neurons ( assessed by cell counts , immunocytochemical analysis of cytoskeletal alterations and ultrastructural studies ) was significantly reduced in animals administered ACTH ( 4 - 9 ) . - pubmed
Deubiquitination of type 2 iodothyronine deiodinase by von Hippel - Lindau protein - interacting deubiquitinating enzymes regulates thyroid hormone activation . - pubmed
The type 2 iodothyronine deiodinase ( D2 ) is an integral membrane ER - resident selenoenzyme that activates the pro - hormone thyroxine ( T4 ) and supplies most of the 3,5,3 '- triiodothyronine ( T3 ) that is essential for brain development . - pubmed
VDU1 , but not VDU2 , is markedly increased in brown adipocytes by norepinephrine or cold exposure , further amplifying the increase in D2 activity that results from catecholamine - stimulated de novo synthesis . - pubmed
The present results show that the blockade of adenosine A2A receptors potentiates the antiparkinsonian effect of L - DOPA . - pubmed
OBJECTIVES : To test the sensitivities of recently published American recommendations predicting occult intracranial lesion ( OICL ) in girls with central precocious puberty ( CPP ) , and to validate a previously derived diagnosis rule predicting OICL based on age at puberty onset and estradiol ( E2 ) level . - pubmed
RESULTS : Ischemic storage per se did not provoke any changes of the surfactant system ; however , severe alterations occurred within 6 hours of reperfusion , resulting in a severe loss of surface activity , including a decrease in the percentage of the large surfactant aggregate fraction , reduction of the surfactant apoproteins SP - B and SP - C , the dipalmitoyl molecular species of phosphatidylcholine and phosphatidylglycerol within the large surfactant aggregate fraction . - pubmed
Calpains are calcium - dependent cysteine proteases and have been implicated in post - ischemic cell death . - pubmed
Whereas leupeptin , CAI-1 or Ac - DEVD - CHO did not modify post - ischemic function , they significantly reduced infarct size and cardiomyocyte positive TUNEL staining . - pubmed
A voltammetric study of direct electron transfer to cytochrome c using a very large assembly of carbon microelectrodes . - pubmed
A very large assembly of more than 8000 carbon fibre microdisk electrodes was used to study direct electron transfer to cytochrome c. - pubmed
Recently described in this context was the dUTPase , which may confer resistance to fluoropyrimidine drugs such as 5 - fluorouracil ( 5 - FU ) . - pubmed
At 28 d of storage , there was a linear ( P & lt ; 0.01 ) increase in Na , K , and Mg content in egg yolks as dietary CLA increased . - pubmed
On d 28 , there was a linear decrease ( P & lt ; 0.01 ) in the Na content of albumen with increasing CLA . - pubmed
Tamoxifen is a well - known antiestrogen used for the hormonotherapy of estrogen receptor positive breast cancer . - pubmed
In addition to its high affinity binding to the estrogen receptor ( ER ) , tamoxifen binds with comparable affinity to the microsomal antiestrogen binding site ( AEBS ) , and inhibits with a micromolar efficiency , protein kinase C ( PKC ) , calmodulin ( CaM)-dependent enzymes and Acyl CoenzymeA : Cholesterol Acyl Transferase ( ACAT ) . - pubmed
These data provide a useful guide for the conception of selective ligands of ERs , AEBS , PKC , CaM or ACAT based on the chemical structure of tamoxifen . - pubmed
The relative activity factor approach assumed that NE-098 formation is predominantly catalysed by CYP3A4 and CYP2C9 and the NE-152 + 163mix ( a mixture of two hydroxylated metabolites , NE-152 and NE-163 ) formation is only catalysed by CYP3A4 . - pubmed
The intrinsic clearance of NE-100 predicted from rCYPs ( the predicted CLint - HLM - total ) corresponded to those observed from EM- and PM - HLM ( the observed CLint - HLM ) . - pubmed
The in vivo oral clearance ( CLoral ) of NE-100 in extensive metabolizers and poor metabolizers of CYP2D6 was predicted to be 50times higher in extensive metabolizers than poor metabolizers using in vitro - in vivo scaling method based on the dispersion model . - pubmed
These data suggest that polymorphism of CYP2D6 might greatly affect NE-100 metabolism in vivo . - pubmed
PURPOSE : 14 - 3 - 3sigma is an intracellular , dimeric , phosphoserine binding protein that is expressed in epithelial cells and involved in cancer development . - pubmed
Here , we describe a new AR variant found in a hormone - refractory metastatic PCa , in which threonine 575 in the DNA binding domain , and threonine 877 in the ligand - binding domain , were both replaced by an alanine . - pubmed
In vitro effect of the potent poly ( ADP - ribose ) polymerase ( PARP ) inhibitor INO-1001 alone and in combination with aspirin , eptifibatide , tirofiban , enoxaparin or alteplase on haemostatic parameters . - pubmed
Since the pathophysiology of acute ischemic cardiac diseases involves haemostatic impairment and the therapeutic regimen includes antithrombotic drugs , we investigated the effect of the potent poly ( ADP - ribose ) polymerase ( PARP ) inhibitor INO-1001 alone and in combination with platelet aggregation inhibitors ( aspirin , eptifibatide and tirofiban ) , unfractionated heparin , low molecular weight heparin ( enoxaparin ) or the recombinant fibrinolytic drug ( alteplase ) , on various haemostatic parameters in vitro . - pubmed
Activated partial thromboplastin time , Anti - Xa activity and euglobulin lysis time were determined in the presence or absence of different concentrations of INO-1001 , in combination with sodium heparin , enoxaparin or alteplase , respectively . - pubmed
Consequently , the PARP inhibitor INO-1001 can be safely applied together with the drugs tested . - pubmed
Immunohistochemistry for PCNA , phospho - IkappaBalpha , and phospho - Erk was also performed . - pubmed
OBJECTIVE : To determine whether clomiphene citrate ( CC ) inhibited gonadotropin - induced ovulation by reducing cyclic adenosine 3 ' , 5 '- cyclic monophosphate ( cAMP ) and prostaglandin E2 ( PGE2 ) levels in ovary . - pubmed
If so , to determine whether E2 coadministration could protect against these effects of CC . - pubmed
MAIN OUTCOME MEASURE(S ): Number of superovulated COCs , ovary and uterus weight , FSH and LH levels in serum , and cAMP and PGE2 levels in ovary . - pubmed
RESULT(S ): The superovulatory dose of gonadotropins significantly increased ovary and uterus weights , cAMP and PGE2 levels in ovary , and serum levels of FSH and LH . - pubmed
Coadministration of CC ( 10 mg / kg body weight ) significantly reduced levels of cAMP , PGE2 in the ovary , ovary and uterus weights , and ovulation rate , whereas FSH and LH levels were not significantly altered . - pubmed
In its center , the chromophore , formed from the tripeptide Gln ( 63 )- Tyr ( 64 )- Gly(65 ) , is tightly held by multiple hydrogen bonds in a polar cage that is structurally quite dissimilar to that of avGFP . - pubmed
In this work we have searched for experimental evidence that would corroborate the idea that nodal threshold ( NT ) is the minimum electric field strength able to promote direct sinus node reset that , in turn , can be verified by a decrease in corrected sinus node recovery time ( CSNRT ) . - pubmed
Immobilization of uricase within polystyrene using polymaleimidostyrene as a stabilizer and its application to uric acid sensor . - pubmed
A novel amperometric uric acid ( UA ) sensor has been developed by coating the surface of a gold electrode with a polystyrene ( PS ) membrane formed by 30 microL of a 30 mg mL(-1 ) PS chloroform solution combined with 30 microL of a 5 mg mL(-1 ) polymaleimidostyrene ( PMS ) solution as a dispersant for enzyme , uricase ; this membrane has been successfully employed as an immobilization support for uricase . - pubmed
In the PS membrane , PMS forms micelle - like structures containing uricase in an active state . - pubmed
This immobilized uricase membrane permits the permeation of oxygen , which is consumed by the uricase reaction . - pubmed
To point out the analytical methodology the dehydration process of l - malic acid , in the presence of Fumarase enzyme , has been used . - pubmed
At the same time hepatic function and blood fat indexes including AST , ALT , Alb , Chol and LDL - C were measured also . - pubmed
On the other hand , LRAE showed some effects in improving the liver function and reducing blood lipids by decreasing the levels of AST , ALT , Chol and LDL - C. All these changes had a significant dose - effect and time - effect relationship . - pubmed
Much of the immune function of S1P results from the engagement of a family of G - protein - coupled receptors ( S1PR1 - S1PR5 ) . - pubmed
Recent findings on the role of S1P in immunosurveillance , the discovery of regulatory mechanisms in S1P - mediated immune - cell trafficking and new advances in understanding the mechanism by which S1P affects immune - cell function indicate that the alliance between S1P and its receptors has a fundamental role in immunity . - pubmed
BACKGROUND & amp ; OBJECTIVE : The present investigation was undertaken to study the iodine nutritional status of school children of Imphal east district in Manipur where endemic goitre persists during post - salt iodization phase along with the investigation of the factors responsible for the occurrence of goitre endemicity . - pubmed
Thus , the existing dietary supplies of thiocyanate in relation to iodine may be a possible aetiological factor for the persistence of endemic goitre in the study region during post salt iodization period . - pubmed
Significant inhibition of viral adsorption onto red blood cells with minimum inhibitory concentrations ranging from 5.3 to 16.8 mM suggested that AL-1 was capable of directly interfering with viral hemagglutinin to block binding to cellular receptors . - pubmed
ADAR1 ( adenosine deaminase acting on RNA ) catalyzes the conversion of adenosine to inosine , a process known as A - to - I editing . - pubmed
Phospho - Stat3 expression was examined by Western blotting and immunofluorescence analysis . - pubmed
PURPOSE : Dynamic contrast - enhanced magnetic resonance imaging ( DCE - MRI ) measured the early vascular changes after administration of TRA-8 , bevacizumab , or TRA-8 combined with bevacizumab in breast tumor xenografts . - pubmed
CONCLUSION : DCE - MRI detected significantly lower K ( trans ) levels in breast tumor xenografts following TRA-8 monotherapy or combined therapy with bevacizumab . - pubmed
In our previous work , hydrogen peroxide is a product of methanol detoxification through alcohol oxidase and NADPH oxidase activation , and acts as a secondary messenger for the activation of ascorbate - related genes . - pubmed
Based on our study , transfusion of ER RBCs before completion of routine blood bank testing carries a low risk of non - ABO alloantibody - mediated HTRs ( 1/265 [ 0.4 % ER episodes ] ) and receipt of antigen - incompatible RBCs ( 7/265 [ 2.6 % ER episodes ] ) . - pubmed
Flash pyrolysis GC - MS was used to examine nitrogen and phenol containing compounds ( M.W.=35 to 450 g / mol ) in the particulate fraction of the milk condensate , combined clean wastewater and aerobic bioreactor effluent . - pubmed
Compounds detected by pyrolysis GC - MS were found mostly in the bioreactor with the amino acid arginine ( 220 mg / kg ) and the amino acid derivative 1 - methyl-5 - oxo - L - proline methyl ester ( 130 mg / kg ) found at the highest concentrations . - pubmed
In comparison , sterols detected in the effluent were found at higher concentrations when using solvent extraction indicating some degradation with pyrolysis GC - MS . - pubmed
METHODS : Fasting plasma glucose ( FPG ) , oral glucose tolerance test ( OGTT ) overload 2 h glucose ( 2hPG ) , glycosylated hemoglobin A1c ( HbA1c ) and fasting insulin ( FIns ) level were measured , respectively . - pubmed
This mutation induced lower protein yield [ ( 12.7 + -1.72 ) mg / L vs ( 16.2 + -2.65 ) mg / L , P<0.01 ] , lower appetency for glucose [ S0.5 : ( 13.96 + -1.89 ) mmol / L vs ( 5.92 + -0.99 ) mmol / L , P<0.001 ] and ATP [ Km:(3.27 + -1.14 ) mmol / L vs ( 0.30 + -0.09 ) mmol / L , P<0.001 ] , lower catalytic ability [ Kcat : ( 1.62 + -0.35)/s vs ( 25.18 + -2.10)/s , P<0.001 ] . - pubmed
Most classical AEDs ( PB , PHT , CBZ , and SV ) have some untoward effect on cognition , particularly , PB . - pubmed
We found a G2203A EGFR mutation , resulting in a G735S amino acid change , in one of 21 ( 5 % ) papillary thyroid cancer samples . - pubmed
The structure of the active site features the facial triad of protein ligands consisting of the side chains of two histidine residues and one aspartate residue that is common to many non - heme Fe ( II ) oxygenases . - pubmed
X - ray absorption spectroscopy ( XAS ) of metallated ( Fe and Ni ) samples of ABH2 was used to investigate the mechanism of ABH2 and its inhibition by Ni ( II ) ions . - pubmed
This aspect is not altered in the Ni ( II )-substituted enzyme , and both metals are shown to bind the cofactor . - pubmed
falciparum induces alterations to the infected RBC morphology and bio - mechanical properties to eventually rupture the host cells through parasitic and host derived proteases of cysteine and serine families . - pubmed
We used previously reported broad - spectrum inhibitors ( E64d , EGTA - AM and chymostatin ) to inhibit these proteases and impede rupture to analyze mechanical signatures associated with parasite escape . - pubmed
Treatment of late - stage iRBCs with E64d and EGTA - AM prevented rupture , resulted in no major RBC cytoskeletal reconfiguration but altered schizont morphology followed by dramatic re - distribution of three - dimensional refractive index ( 3D - RI ) within the iRBC . - pubmed
We show that E64d and EGTA - AM supported PV breakdown and the resulting elevated fluctuations followed non - Gaussian pattern that resulted from direct merozoite impingement against the iRBC membrane . - pubmed
ER - directed treatments include lowering ligand concentration by using aromatase inhibitors ( AI ) and blocking the receptor with agents like tamoxifen ( TAM ) or fulvestrant ( FUL ) . - pubmed
RESULTS : As expected , tumor FES uptake declined more markedly on ER blockers ( TAM and FUL , average 54 % decline ) compared with a less than 15 % average decline on estrogen - depleting AIs ( P & lt ; 0.001 ) . - pubmed
INTERVENTION(S ): Daily gonadotropin - releasing hormone antagonist ( GnRH , 0.25 mg ) used from day 6 of stimulation onward , final oocyte maturation triggered by administration of 10,000 IU of human chorionic gonadotropin ( hCG ) as soon as & gt;= 3 follicles & gt;= 17 mm were present , and hormone assessment performed at initiation of stimulation , on the first day after menstruation had stopped , on the day of antagonist initiation , and on the day of hCG administration . - pubmed
CONCLUSION(S ): In recombinant follicle - stimulating hormone / gonadotropin - releasing hormone antagonist in vitro fertilization / intracytoplasmic sperm injection cycles , a lower probability of pregnancy is associated with a higher exposure to progesterone during the menstruation - free interval . - pubmed
However , membrane cholesterol also plays a role in the formation and aggregation of amyloid - beta . - pubmed
During the fifth year of follow up , incident cardiovascular diseases , ABI , and mortality were documented , and related to ACE genotype adjusting for age , smoking , alcohol , dietary intake , physical activity , body mass index , and use of ACE inhibitors . - pubmed
A reporter gene construct employing a synthetic oestrogen response element predicted to specifically interact with oestrogen receptors ( ER ) was constructed . - pubmed
Porous and trigonal TiO ( 2 ) nanoflakes ( p - TiO ( 2 ) ) have been synthesized via a simple hydrothermal calcination process , and characterized by X - ray diffraction ( XRD ) , transmission electron microscopy ( TEM ) , and UV - vis absorption / reflection spectroscopy ( UV - vis ) . - pubmed
Inhibition of mitochondrial complex I by various non - steroidal anti - inflammatory drugs and its protection by quercetin via a coenzyme Q - like action . - pubmed
A major objective of the present study was to investigate whether in vitro the NSAIDs , aspirin , indomethacin , diclofenac , piroxicam and ibuprofen , which feature different chemical structures , are able to inhibit mitochondrial complex I. - pubmed
In addition , the present study shows that quercetin ( 5 - 10 muM ) can behave as a " coenzyme Q - mimetic " molecule , allowing a normal electron flow along the whole electron transporting chain ( complexes I , II , III and IV ) . - pubmed
The organic hydroperoxide resistance protein Ohr has been identified in numerous bacteria where it functions in the detoxification of organic hydroperoxides , and expression of ohr is often regulated by a MarR - type regulator called OhrR. - pubmed
The effect of oscillatory decrease in the heart rate and hemoglobin oxygen saturation ( SaO2 ) during the submaximal workload ( bicycle ergometry test ) was demonstrated in high - performance athletes and volunteers practicing healthy life style . - pubmed
Absence of both MDR1 ( ABCB1 ) and breast cancer resistance protein ( ABCG2 ) transporters significantly alters rivaroxaban disposition and central nervous system entry . - pubmed
Rivaroxaban is a novel factor 10a inhibitor , where hepatic metabolism and renal clearance account for its overall disposition . - pubmed
As renal rivaroxaban clearance exceeds glomerular filtration rate , we suggested that active secretion by efflux transporters P - glycoprotein ( MDR1 ) and breast cancer resistance protein ( BCRP ) contributes to rivaroxaban clearance . - pubmed
The ability of MDR1 and BCRP efflux transporters to mediate rivaroxaban transport in vitro was assessed in polarized cell monolayers . - pubmed
Rivaroxaban is a shared substrate of MDR1 and BCRP . - pubmed
In vivo , MDR and BCRP function synergistically to modulate rivaroxaban disposition and appear to be particularly relevant to limiting its central nervous system entry . - pubmed
These data have important implications for safety and efficacy of anticoagulation therapy with rivaroxaban as many drugs in clinical use are known MDR1 inhibitors and loss - of - function polymorphisms in BCRP are common . - pubmed
We have here demonstrated for the first time that nitrate not only accelerates testa rupture of non- AR seeds but also modifies expression pattern of the cell - wall remodeling proteins ( mannanases ; SoMAN6 and SoMAN7 ) and key genes belonging to metabolism and signaling of ABA ( SoNCED6 , SoNCED9 , SoCYP707A2 and SoABI5 ) and GAs ( SoGA3ox , SoGA20ox , SoGA2ox and SoRGL2 ) . - pubmed
The expression of SoMAN6 and SoMAN7 were positively altered by nitrate . - pubmed
Although ABA synthesis seems apparent at the start of non - AR seed imbibition , taken together the results of SoNCED6 , SoNCED9 and SoCYP707A2 expression seem to suggest that nitrate leads to a strong net ABA decrease . - pubmed
Likewise , nitrate positively affected the SoABI5 expression when the SoNCED9 expression was also stimulated . - pubmed
By contrast , at the early and final of imbibition , nitrate clearly inhibited the SoABI5 expression . - pubmed
The expression of SoGA2ox6 and SoGA3ox2 are strongly inhibited by nitrate whereas of SoGA20ox6 was stimulated . - pubmed
On the other hand , SoRGL2 transcript level decreased in the presence of nitrate . - pubmed
The nitrate , in cross - talk with the AR network likely increases the favorable molecular conditions that trigger germination . - pubmed
Using the nano - porous pseudo carbon paste electrode ( Nano - PPCPE ) as the working electrode , mixing L - glutamate oxidase ( L - GLOD ) , catalase ( Cat ) and bovine serum albumin ( BSA ) with phosphate buffer ( PB , pH = 7.4 ) , followed by cross - linking with glutaraldehyde , a novel L - glutamate electrochemical biosensor was successfully formed . - pubmed
Cytotoxicity was evaluated using MTT reduction , lactate dehydrogenase leakage and neutral red uptake assays , for different concentrations and combinations of both agents , at 24 and 48 h. Apoptosis was also assessed using fluorescence microscopy and through the evaluation of caspases 8 , 9 , and 3 activities . - pubmed
Administration of either bovine thyrotropic hormone ( bTSH ) or ovine growth hormone ( oGH ) had no effect on thyroid hormone content of larvae challenged during the yolk absorption period , suggesting that the thyroid tissue was not responsive to exogenous bTSH challenge at this time , and that oGH - sensitive 5'-monodeiodination was either not present or at levels that were too low to cause an elevation in total T3 content , or that the substrate levels were insufficient to permit a measureable increase in whole body T3 content . - pubmed
In this regard , the purpose of this Forum Article is to more visibly highlight the significant contributions of inorganic chemistry to nuclear imaging by detailing the development of five metal - based imaging agents : ( 64 ) Cu - ATSM , ( 68 ) Ga - DOTATOC , ( 89 ) Zr - transferrin , ( 99m ) Tc - sestamibi , and ( 99m ) Tc - colloids . - pubmed
In general the substances which enhanced cell elongation also enhanced the development of invertase , but generally they did not enhance the increase in peroxidase activity or have any effect on the decrease in RNA content.[( 14 ) C ] cinnamic acid was continuously incorporated into the cell walls of elongating segments from which it could only be partially removed with NaOH , pronase , or HClO4 . - pubmed
Here , we present a new strategy applying MALDI - TOF MS in positive ion mode for semi - qualitative and quantitative analysis of CS / DS derived disaccharide units . - pubmed
The levels of alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , total cholesterol ( TC ) , triglyceride ( TG ) , high density lipoprotein cholesterol ( HDL - C ) , low density lipoprotein cholesterol ( LDL - C ) were detected in the serum and liver tissue ; Changes in fat deposition in liver tissue were determined by oil red O staining . - pubmed
The molecular mechanisms underlying the targets of curcumin are diverse and involve combinations of multiple signaling pathways , including NF - kappaB and STAT3 signaling . - pubmed
It was established that in all experimental versions rapidly absorbed from the culture medium amino acids : cysteine and cystine , serine and aspartic acid , valine and tryptophan , proline and hydroxyproline , which are involved in the synthesis of proteins , in particular collagen . - pubmed
This mutation ( ' DeltaE ' ) removes a single glutamic acid from the encoded protein , torsinA. - pubmed
Based on the PQA adherence measure ( PDC & gt;=0.8 ) , a significantly higher proportion of rivaroxaban users ( 72.7 % ) was found to be adherent compared to dabigatran ( 67.2 % : p & lt ; 0.001 ) and apixaban ( 69.5 % : p & lt ; 0.001 ) users . - pubmed
Adipocyte - like differentiation was confirmed by a combination of positive Oil Red O and adipophilin immunohistochemical staining . - pubmed
Substitution of phenylalanine residues by tryptophan introduced endotoxin neutralization capacity in inactive truncated CATH-2 derived peptides . - pubmed
In contrast , amino acid substitution of phenylalanine by tyrosine abrogated endotoxin neutralization activity of CATH-2 analogs . - pubmed
The anthrax lethal factor ( LF ) is a zinc dependent metalloproteinase that cleaves the majority of mitogen - activated protein kinase kinases and a member of NOD - like receptor proteins , inducing cell apoptosis . - pubmed
Tannase - mediated biotransformation assisted separation and purification of theaflavin and epigallocatechin by high speed counter current chromatography and preparative high performance liquid chromatography : A comparative study . - pubmed
A large scale isolation and purification of theaflavin ( TF ) and epigallocatechin ( EGC ) has been successfully developed by tannase - mediated biotransformation combining high - speed countercurrent chromatography . - pubmed
After tannase hydrolysis of a commercially available theaflavins extract ( TE ) , the content of TF and EGC in tannase - mediated biotransformation product ( TBP ) achieved approximately 3 times enrichment . - pubmed
OBJECTIVE : The objective of this study was to investigate the relationship between level of 18F - fluorodeoxyglucose ( 18F - FDG ) accumulation in primary breast tumour assessed by positron emission tomography / computed tomography ( PET / CT ) and histopathological and clinical prognostic factors . - pubmed
Transient receptor potential cation channel , subfamily C , member 1 ( TRPC1 ) activates Ca2 + entry upon store depletion in a variety of cell types . - pubmed
Moreover , knockdown of TRPC1 decreased Ca2 + entry to the cytoplasm of hDPCs . - pubmed
In wild - type reaction centers , the triplet energy transfer efficiency from P to the carotenoid was essentially unity at room temperature and at 77 K. However , in the M182HL mutant reaction centers , both the rate and efficiency of triplet energy transfer were decreased at room temperature , and at 77 K , no triplet energy transfer was observed , attributable to a higher triplet state energy of the bacteriopheophytin that replaces bacteriochlorophyll in this mutant . - pubmed
In this work , a fully tin - based , mixed - organic - cation perovskite absorber ( FA ) x ( MA)1 - x SnI3 ( FA = NH2CH = NH2 + , MA = CH3NH3 + ) for lead - free perovskite solar cells ( PSCs ) with inverted structure is presented . - pubmed
By optimizing the ratio of FA and MA cations , a maximum power conversion efficiency of 8.12 % is achieved for the ( FA)0.75(MA)0.25SnI3 - based device along with a high open - circuit voltage of 0.61 V , which originates from improved perovskite film morphology and inhibits recombination process in the device . - pubmed
An insulin tolerance test was used unless contraindicated , then the GHRH - arginine test and Synachten test were used . - pubmed
GABA and malate appear to interact with ALMTs in a complex manner to regulate each other 's transport , suggestive of a role for ALMTs in communicating metabolic status . - pubmed
Finally , the alkaline phosphatase activity of the cells was found to increase with increasing content of TCP , which provides an encouraging proof of concept for the use of curcumin - loaded synthetic biomaterials in bone remodeling . - pubmed
Over - expression of AtPAP2 resulted in faster growth and increased biomass in several plant species , indicating its great potential for crop improvement of phosphate use and yield . - pubmed
Glutathione superoxide dismutase , antioxidant capacity , and total superoxide dismutase were higher , whereas malondialdehyde was lower in F group than in C and U groups from 1 to 21 days and from 22 to 42 days ( P & lt ; 0.05 ) . - pubmed
Nitrogenase - like enzymes play a vital role in the reduction of the conjugated ring systems of diverse tetrapyrrole molecules . - pubmed
The biosynthesis of all bacteriochlorophylls involves the two - electron reduction of the C7 - C8 double bond of the green pigment chlorophyllide , which is catalyzed by the nitrogenase - like two - component metalloenzyme chlorophyllide oxidoreductase ( COR ) ; whereas in all methanogenic microbes , another nitrogenase - like system , CfbC / D , is responsible for the sophisticated six - electron reduction of Ni2 + - sirohydrochlorin a , c - diamide in the course of coenzyme F430 biosynthesis . - pubmed
The first part of this chapter describes the production and purification of the COR components ( BchY / BchZ ) 2 and BchX2 , the measurement of COR activity , and the trapping of the ternary COR complex ; and the second part describes the strategy for obtaining homogenous and catalytically active preparations of CfbC2 and CfbD2 and a suitable method for extracting the reaction product Ni2 + - hexahydrosirohydrochlorin a , c - diamide . - pubmed
The development of somatostatin analogs for the treatment of pituitary Cushing ' s disease has been based on somatostatin receptor expression analyses of small cohorts of pituitary adenomas . - pubmed
SSTR2A , SSTR3 , and SSTR5 seem to be most suitable biomarkers for a targeted therapy with somatostatin analogs . - pubmed
Compound alpha - keto acid tablet supplementation alleviates chronic kidney disease progression via inhibition of the NF - kB and MAPK pathways . - pubmed
7 - Ethyl-10 - hydroxy - camptothecin ( SN-38 ) , an efficient topoisomerase inhibitor , is the biological metabolite of irinotecan used as the first - line chemotherapy drug for colon cancer . - pubmed
SN-38 - BOC micelles could accumulate in tumors due to the EPR effect and exhibit a sustained release behavior , which was rapidly transformed to active SN-38 by the acidic environment of tumor tissues ( pH 5.5 - 6.8 ) , thus achieving efficient anti - tumor activity . - pubmed
Up - regulation of HDACs , a harbinger of uraemic endothelial dysfunction , is prevented by defibrotide . - pubmed
Both the HDACs inhibitor trichostatin A and DF prevented the up - regulation of the endothelial dysfunction markers induced by the uraemic milieu : intercellular adhesion molecule-1 , surface Toll - like receptor-4 , von Willebrand Factor and reactive oxygen species . - pubmed
HDACs appear as key modulators of the CKD - induced endothelial dysfunction as specific blockade by trichostatin A or by DF prevents endothelial dysfunction responses to the CKD insult . - pubmed
Previous studies have shown that the carbonic anhydrase inhibitor ( CAI ) dorzolamide can induce immediate dilatation of retinal arterioles and a sustained increase in retinal blood flow in primary open - angle glaucoma . - pubmed
Li81 has the unusual feature that it contains two LINGO-1 binding sites : a classical site utilizing its complementarity - determining regions and a cryptic secondary site involving Li81 light chain framework residues that recruits a second LINGO-1 molecule only after engagement of the primary binding site . - pubmed
tuberculosis and/or HIV - infection and investigated TB antibody reactivities , function , and other host protein biomarkers in simultaneously obtained serum and plasma from serum separation , EDTA , heparin , acid citrate dextrose ( ACD ) , or mononuclear cell preparation ( CPT ) tubes which contain heparin and Ficoll . - pubmed
However , the presence of ACD resulted in slightly lower values than those obtained with serum in both indirect ( antibody reactivities to mycobacterial antigens ) and Sandwich ELISAs ( soluble CD14 measurements ) . - pubmed
The results revealed the adsorption equilibrium of rhodamine B ( RhB ) and Congo red ( CR ) onto RFB550 with initial concentrations of 30 mg L-1 at 25 C and 210 min , and the best adsorption was observed when the pH of the RhB and CR solutions was 3 and 5 , respectively . - pubmed
Pseudo - second - order kinetics was the most suitable model for describing the adsorption of RhB and CR onto RFB550 , indicating that the rate - limiting step was mainly chemical adsorption . - pubmed
Thermodynamic parameters revealed the spontaneous adsorption of RhB and CR onto RFB550 . - pubmed
According to the results of the FTIR analysis , the oxygen - containing functional groups and aromatic structures on the surface of RFB550 provided abundant adsorption sites for RhB and CR , and the adsorption mechanism was potentially related to the hydrogen bonds and pi - pi bonds . - pubmed
We show here that during the early stage of viral infection , the acetylation of TBK1 was increased , and the acetylation of TBK1 at Lys241 enhanced the recruitment of IRF3 to TBK1 . - pubmed
HDAC3 directly deacetylated TBK1 at Lys241 and Lys692 , which resulted in the activation of TBK1 . - pubmed
Deacetylation at Lys241 and Lys692 was critical for the kinase activity and dimerization of TBK1 respectively . - pubmed
Here , we developed a two - part repair strategy comprising a dual - modified ( oxidized and methacrylated ) glycosaminoglycan that can chemically adsorb an injectable interpenetrating network hydrogel composed of fibronectin - conjugated fibrin and poly ( ethylene glycol ) diacrylate ( PEGDA ) to covalently bond the hydrogel to AF tissue . - pubmed
The concentrations of exposed PM2.5 , PM10 , NO2 and O3 in the PE group were significantly higher than those in the non - PE group , and they were risk factors of the PE group in the first and second trimester of pregnancy respectively . - pubmed
As a result , the adsorption capacity of Cd2 + on MFB ( 17.4 mg g-1 ) was more than two times higher than that on FB ( 7.2 mg g-1 ) , and the adsorption of Cd2 + on MFB was controlled by physisorption and chemisorption . - pubmed
The effect of MFB on the bioavailability of Cd was investigated using a leaching test ( the European standard EN 12457 - 2 ) combined with a typical sequential extraction procedure ( the community bureau of reference , BCR ) . - pubmed
, thiazole ) should be introduced to P1 ' and P4 sites in order to enhance H - bonds and hydrophobic interactions , respectively . - pubmed
By contrast , individuals with MetS had lower high - density - lipoprotein cholesterol ( HDL - C ) counts and FPIS . - pubmed
It appears that immunoreactivity of beta - endorphin resides in the COOH - terminal segment of residues ( 22 - -31 ) . - pubmed
The data reported here indicate the presence of B - cell epitopes within the carboxy - terminal third of the gp340 polypeptide chain . - pubmed
Occurrence of an allosteric transition in the modification of papain with L-1 - acetyl-2,3 - dihydropyrrolo [ 2,3 - b ]- indole-2 - carboxamide . - pubmed
When papain was reacted with L-1 - acetyl-2,3 - dihydropyrrolo [ 2,3 - b ] indole - 2 - carboxamide at pH 8.0 , inactivation occurred accompanied by modification of Cys-25 in the active site . - pubmed
This is supported by the fact that the presence of a moderate concentration ( a twenty - fold molar excess ) of N alpha - acetyl - L - tryptophanamide over papain accelerated the inactivation . - pubmed
The extracytoplasmic region of the 270 - kDa mannose 6 - phosphate / IGF - II receptor is composed of 15 repeating domains and is capable of binding 2 mol of mannose 6 - phosphate ( Man-6 - P ) . - pubmed
These adrenal responses were blocked by dexamethasone ( DEX ) suggesting that the effect of 4 - APP occurred indirectly via stimulation of the adrenal gland by endogenous adrenocorticotrophic ( ACTH ) . - pubmed
In all the above experiments , adrenal gland cholesterol content and apoE mRNA concentration were positively correlated ( r = 0.78 , P = 0.0001 ) . - pubmed
In contrast , aminoglutethimide treatment , which blocks adrenal gland steroidogenesis and greatly increases adrenal gland cholesterol content , was without effect on apoE mRNA concentration . - pubmed
This uncoupling of adrenal gland cholesterol content and apoE mRNA concentration suggests that apoE mRNA expression and cellular cholesterol are regulated independently by ACTH . - pubmed
Phosphoaminoacid analysis indicated that pp19 is dephosphorylated on serine residues . - pubmed
DDC forms chelates with copper and the microbicidal effectiveness of this drug is enhanced greatly by small amounts of copper . - pubmed
E loss , and LDH leakage induced by Ca2 + omission from the incubation medium . - pubmed
Anaemia of rheumatoid arthritis : serum erythropoietin concentrations and red cell distribution width in relation to iron status . - pubmed
The mean serum erythropoietin level ( 57.6 ( SD ) 27.3 ) U / l ) was sufficiently raised for the degree of anaemia irrespective of the size of the marrow iron stores . - pubmed
The plasma lactoferrin concentration ( mean ( SD ) 137.6 ( 109.9 ) micrograms / l ) was normal and did not differ significantly between the iron deficient patients and those with adequate iron . - pubmed
The positive inotropic effects of the bipyridine derivatives amrinone and milrinone and of the benzimidazole compounds sulmazole , pimobendane , and UD - CG 212 Cl are due at least in part to inhibition of cardiac phosphodiesterase activity and hence to an increase in myocardial cyclic AMP ( cAMP ) content . - pubmed
Influence of sympathetic discharge pattern on norepinephrine and neuropeptide Y release . - pubmed
After blockade of alpha - and beta - adrenoceptors by phenoxybenzamine and propranolol , respectively , which enhanced nerve stimulation - evoked overflow of both NE and NPY - LI , the NE overflow from the muscle evoked by the irregular activity was slightly larger at 0.59 Hz but smaller at higher frequencies compared with that evoked by regular activity , whereas the detectable overflow of NPY - LI was not largely influenced by the stimulation pattern . - pubmed
The hydroxyl radical has been spin trapped in microsomal and purified NADPH - cytochrome P-450 reductase systems in the presence of adriamycin , daunomycin and mitomycin C. - pubmed
The hydroxy radical generation has been stimulated by the Fe - EDTA complex and completely inhibited by catalase . - pubmed
Strain differences in pituitary prolactin content : relationship to number of hypothalamic dopamine neurons . - pubmed
Strain differences in the synthesis and release of prolactin would also be predicted since dopamine released from the tuberoinfundibular neurons is the prolactin inhibitory factor which plays a role in the regulation of both prolactin synthesis and release . - pubmed
We conclude that strain differences in the number of tuberoinfundibular dopamine neurons are inversely related to the number of immunocytochemically demonstrable prolactin - containing cells in the anterior pituitary . - pubmed
[ Effect of the humic and fulvic acids of sapropel on the NAD.H - oxidase activity of the liver mitochondria ] . - pubmed
Nuclease P1 - mediated enhancement of sensitivity of 32P - postlabeling test for structurally diverse DNA adducts . - pubmed
In the standard procedure , DNA is enzymatically digested to 3'-phosphorylated normal and adducted mononucleotides , which are quantitatively 32P - labeled at their 5 '- hydroxyl groups by T4 polynucleotide kinase - catalyzed [ 32P]phosphate transfer from [ gamma-32P ] ATP . - pubmed
Nuclease P1 cleaves deoxyribonucleoside 3 '- monophosphates of normal nucleotides to deoxyribonucleosides which do not serve as substrates for polynucleotide kinase , while most adducted nucleotides were found to be totally or partially resistant to the 3'-dephosphorylating action of nuclease P1 . - pubmed
Amino acid sequencing of the N termini of the pre - hGH and pho - hGH gene products revealed that both were processed correctly . - pubmed
Electrophoretic analysis of these polypeptides on reducing and nonreducing sodium dodecyl sulfate ( SDS)-polyacrylamide ( PA ) gels indicates that periplasmic hGH is monomeric and contains the same two disulfide bonds as authentic hGH . - pubmed
Assignments of the six sets of aromatic ring protons and four high - field - shifted methyl group protons of the C - terminal fragment of calmodulin , residues 78 - 148 , was achieved by a combination of one and two - dimensional NMR spectroscopic methods . - pubmed
In the glomerular layer , methionine - enkephalin immunoreactivity is observed in the somata and dendrites of periglomerular cells whereas substance P immunoreactivity is present in the somata and dendrites of external tufted cells . - pubmed
In the deeper layers , methionine - enkephalin immunoreactivity is observed in a mixed population of neurons which appear to be primarily granule cells , whereas somatostatin immunoreactivity is present only in deep short axon cells . - pubmed
Methionine - enkephalin- , substance P- , and somatostatin - positive fibers of central origin are also observed in the main olfactory bulb . - pubmed
The cells and culture medium were collected , and radioactive proline incorporated into collagen and noncollagen protein was determined . - pubmed
Prolyl hydroxylase activity was also increased significantly in the bleomycin - treated cells . - pubmed
Free and peptide - bound radioactive hydroxyproline in the cells and medium was greatly increased in bleomycin - treated cells , which indicates increased collagen degradation . - pubmed
The results demonstrate that , although collagen synthesis in lung fibroblasts is increased by bleomycin , the newly synthesized collagen is rapidly degraded in both the cell layer and the medium . - pubmed
All hand wounds should be cultured aerobically and anaerobically and empiric antibiotic therapy should include a penicillinase - resistant penicillin or cephalosporin in combination with penicillin G. - pubmed
Prolonged combined in vivo pre - treatment with luteinizing hormone - releasing hormone ( LRH ) and oestradiol benzoate causes long - lasting suppression of the autonomous and the LRH - stimulated secretion of luteinizing hormone and follicle stimulating hormone . - pubmed
The in vivo distribution of iron between the binding sites of transferrin was examined . - pubmed
Independent methods , including measurement of the transferrin profile after isoelectric focusing and cross immunoelectrophoresis , and determination of the iron content in the separated fractions were in agreement that there was a random distribution of iron on binding sites . - pubmed
The data indicate that the distribution of apo - , monoferric , and diferric transferrins is predictable on the basis of the plasma transferrin saturation and negate the concept that iron loading of transferrin in vitro is a selective process with possible functional consequences in tissue iron delivery . - pubmed
The proteinase activity was completely inhibited by di - isopropyl fluorophosphate , leupeptin and antipain , indicating that the proteinase bound to alpha 1 - macroglobulin is a serine proteinase . - pubmed
Two protein bands ( 62 and 59 kDa ) of the complex were labelled with [ 3H ] diisopropyl fluorophosphate and both bands cross - reacted with anti -( mast - cell tryptase ) antibody . - pubmed
In the lean subjects , tyrosine phosphorylation of the insulin receptor and insulin receptor substrate-1 ( IRS-1 ) , measured by immunoblotting with anti - phosphotyrosine antibodies , was significantly increased by insulin at all time points . - pubmed
RESULTS : All compounds examined in this study showed high and similar affinity for alpha 1 adrenoceptor subtypes , with the exception of 5 - Methyl - urapidil , which was selective for alpha 1C ( pKi = 9.3 ) over alpha 1B ( pKi = 7.2 ) and alpha 1A ( pKi = 8.1 ) . - pubmed
Doxazosin , terazosin , alfuzosin , and tamsulosin were potent antagonists of phenylephrine responses and in vivo derived " pseudo pA2 " determinations showed that the drugs did not discriminate between prostatic and vascular receptors . - pubmed
Facilitation of ICa was not altered ( when present ) or favoured ( when absent ) by the cAMP - dependent phosphorylation of Ca2 + channels promoted by isoprenaline or by intracellular application of cAMP or of the catalytic subunit of protein kinase A ( C - sub ) . - pubmed
Following intracellular application of inhibitors of protein kinase A in combination with a non - hydrolysable ATP analogue , ICa consisted predominantly of ICa , sc and no facilitation was observed . - pubmed
The calmodulin antagonist naphthalenesulphonamide had no effect on facilitation . - pubmed
Inositol trisphosphate / Ca2 + as a major signal transduction pathway from bradykinin receptors . - pubmed
The initial signal transduction from bradykinin receptors to acetylcholine secretion is composed of the inositol-1,4,5 - trisphosphate - dependent Ca2 + pathway despite multiple pathways in the late phase . - pubmed
The interaction of the B1 domain with IgGFc was investigated by 1H-15N correlation spectroscopy . - pubmed
diabetes ( RR = 1.67 and 1.53 in men and women , respectively ) , serum cholesterol , low density lipoprotein cholesterol and serum apo B in men ( RR = 1.24 ) , serum triglycerides in women ( RR = 1.23 ) , high density lipoprotein cholesterol in men ( RR = 0.82 ) , lipoprotein ( a ) in women ( RR = 1.99 ) , and poorer self - rating of health ( RR = 1.48 and 1.93 in men and women , respectively ) . - pubmed
Approach to genotyping errors caused by nontemplated nucleotide addition by Taq DNA polymerase . - pubmed
Thermostable DNA polymerases can catalyze nontemplated addition of a nucleotide to the 3 ' end of amplification products . - pubmed
These findings indicate that the mutation is specific for cytochrome c oxidase and does not affect the biosynthesis of heme A. - pubmed
We found that the BrdU in vivo method , coupled with bivariate FCM for measurements , is a valuable approach for a ' dynamic ' assessment of tumour cell proliferation in the clinical setting . - pubmed
Galactose exposed lenses both in vitro and in vivo showed a 5 - 9 - fold increase in crystallin bound Schiff base - linked radioactivity when incubated with 1 - 14C - labeled ASA or DHA . - pubmed
The mutagenic effect was found only in acetone extracts of urinary filtrate in the presence of promutagenic activating fraction S9 . - pubmed
Based on these findings , rhizoxin , a microbial metabolite that contains two epoxide groups and exhibits anti - tubulin activity , was tested for anti - angiogenic activity in a CAM assay system . - pubmed
The two migration inhibitory factor - ( MIF )- related protein-8 ( MRP8 ; S100A8 ) and MRP14 ( S100A9 ) are two calcium - binding proteins of the S100 family . - pubmed
This review addresses recent developments regarding the MRP14 - mediated promotion of leukocyte - endothelial cell - interactions and the characterization of MRP8 / 14 heterodimers as a fatty acid binding protein complex . - pubmed
Sample lipooligosaccharides ( LOS ) , was prepared by a modification of the hot phenol - water method from which de - O - acetylated LOS and oligosaccharide ( OS ) components were analyzed by ES - MS - CID - MS and ES - MSnin a triple quadrupole and an ion trap mass spectrometer , respectively . - pubmed
Mild acid hydrolysis to release OS also hydrolyzed the beta(1 - ->6 ) glycosidic linkage of lipid A. OS structures were determined by collisional and resonance excitation combined with MS and multistep MSn which provided sequence information from both neutral loss , and nonreducing terminal fragments . - pubmed
With intrapipette ( -)Bay K 8644 ( 1 microM ) , mean open time was best fit by two exponentials , tau(of ) = 0.9+/-0.2 msec ( n = 10 ) and tau(os ) = 13.4+/-2.6 msec ( n = 10 ) ; mean close time values were tau(cf ) = 0.6+/-0.1 msec ( n = 10 ) and tau(cs ) = 9.8+/-1.9 msec ( n = 10 ) , respectively . - pubmed
However , CGP 2881 consistently inhibited CuSO4 ( Cu2 +)- and macrophage ( MO)-induced oxidation of human low density lipoproteins ( LDL ) more potently than equimolar concentrations of probucol . - pubmed
CGP 2881 ( 1 mumol / l ) prolonged the lag phase of diene formation during Cu ( 2 +)-induced LDL oxidation by 3.4 versus 1.5 - fold prolongation with 1 mumol / l probucol ( P & lt ; 0.05 vs CGP 2881 ) . - pubmed
The IC50 for MO - induced oxidation of LDL ( TBARS ) was 0.64 mumol / l. In contrast , 1 mumol / l probucol failed to inhibit MO - induced oxidation of LDL . - pubmed
Five days of orally administered CGP 2881 reduced plasma total cholesterol and LDL cholesterol levels to 55 and 54 % of vehicle - treated controls , respectively ( P & lt ; 0.05 ) . - pubmed
In contrast , probucol had no appreciable effect on plasma total cholesterol or LDL cholesterol levels , unless administered for longer than 5 days . - pubmed
CGP 2881 may be useful as a new antioxidant tool in the effort to better understand the atherogenicity of oxidized LDL ( oxLDL ) . - pubmed
Photoregulation of the DNA Polymerase Reaction by Oligonucleotides Bearing an Azobenzene We should like to thank Professor Kazunari Taira for valuable comments . - pubmed
Computer simulations are presented that resolve the paradoxical finding that while ACE inhibitors are effective , genetic variations in the expression of the ACE gene do not affect blood pressure . - pubmed
The DES - induced change in AR immunoexpression was confirmed by Western analysis for the testis . - pubmed
Treatment - induced changes in AR expression were paralleled by changes in Leydig cell volume per testis ( 91 % reduction in the 10 - microg DES group ; no change in the 1 - microg DES group ) . - pubmed
Coadministration of TE with DES prevented DES - induced loss of AR immunoexpression ( confirmed for testis by Western blot analysis ) . - pubmed
The peroxiredoxins ( Prx ) are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin ( Trx ) or other substances . - pubmed
Expression of the insulin gene is highly specific to pancreatic beta cells and is upregulated mainly by PDX-1 and BETA2 / NeuroD depending on the extracellular glucose concentration . - pubmed
Reporter gene analyses using pancreatic HIT - T15 cells revealed that the glucose - dependent insulin promoter activity was blocked by glucocorticoids , dexamethasone ( DEX ) and hydrocortisone , in a dose - dependent manner . - pubmed
Moreover , because CGP 52608 is a ligand of RZR / ROR receptors and the latter are considered by some investigators as nuclear binding sites for melatonin , our data suggest the involvement of these receptors in the pro - apoptotic effect of melatonin . - pubmed
Structural basis for the recognition of isoleucyl - adenylate and an antibiotic , mupirocin , by isoleucyl - tRNA synthetase . - pubmed
A structural comparison of the IleRS.Ile - AMS complex with the adenylate complexes of other aminoacyl - tRNA synthetases revealed the common recognition mode of aminoacyl - adenylate by the class I aminoacyl - tRNA synthetases . - pubmed
The Ile - AMS and mupirocin , which have significantly different chemical structures , are recognized by many of the same amino acid residues of the IleRS , suggesting that the antibiotic inhibits the enzymatic activity by blocking the binding site of the high energy intermediate , Ile - AMP . - pubmed
In contrast , the two amino acid residues that concomitantly recognize Ile - AMS and mupirocin are different between the eubacterial / archaeal IleRSs and the eukaryotic IleRSs . - pubmed
AChE staining was located in the endoplasmic reticulum of geniculate ganglion neuronal somata , and extracellularly , surrounding labeled chorda tympani terminal fibers and boutons in the NST . - pubmed
AChE staining was reduced in the dextran - labeled chorda tympani fibers and terminals as well as adjacent non - labeled profiles 2 weeks following nerve transection and dextran application . - pubmed
Within the composition of the ration , the levels of rumen degradable protein ( RDP ) and non - structural carbohydrates ( NSC ) were changed ( diet H : 74 % RDP and 38 % NSC ; diet M : 57 % RDP and 32 % NSC ; diet L : 48 % RDP and 23 % NSC ) . - pubmed
Different test agents : muscle stimulation ( MS ) , arterial injection of KCl or lactic acid and tendon vibrations , were used to activate muscle afferents . - pubmed
Both acute and chronic hypoxaemia suppressed the post - MS activation of groups III and IV muscle afferents and significantly depressed the response of these afferents to KCl and lactic acid . - pubmed
Penzo ( a ) pyrene , 7,12 - dimethyl benz ( a ) anthracene and pentacene effectively inhibit the specific 3H - estradial binding to rat uterine estrogen receptor(P & lt ; 0.01 ) . - pubmed
Whereas benz ( a ) anthracene and chrysene can not inhibit the specific 3H - estradial binding to rat uterine estrogen receptor(P & gt ; 0.05 ) . - pubmed
Insulin affects not only glucose metabolism , but also protein synthesis and cell growth . - pubmed
Insulin - induced tyrosine phosphorylation of insulin receptor beta subunit ( IRbeta ) and insulin receptor substrate-1 ( IRS-1 ) and tyrosine / threonine phosphorylation of p44 / 42 MAPK ( ERK-1 / 2 ) were evaluated . - pubmed
However , tyrosine phosphorylation of IRS-1 in the soleus muscle of FFR was significantly reduced to 80 % ( p<0.001 ) of that in controls . - pubmed
Gelatin - containing native - PAGE gels indicated the presence of several aspartyl , cysteine , and serine protease forms and confirmed the spatial organization of the proteolytic digestive process . - pubmed
Serum parathyroid hormone was suppressed , 25 - OH vitamin D was within the normal range , and 1,25 -( OH )( 2 ) vitamin D was elevated . - pubmed
This study evaluated the effect of fenoldopam , a selective dopamine ( DA1 ) agonist , on renal blood flow and renal tubular function following renal ischemia induced by suprarenal aortic cross - clamping . - pubmed
The mean creatinine increase from baseline at 6 hr and 18 hr after cross - clamp removal for the fenoldopam - treated group was significantly less than that in the control group ( p & lt ; 0.001 ) . - pubmed
METHODS : Claims data from a large US managed care organization from July 1 , 1998 , through June 30 , 2000 , were analyzed for adult members with continuous enrollment , & gt;or=1 prescription drug claim , & gt;or=2 sets of fasting low - density lipoprotein cholesterol ( LDL - C ) laboratory results , and no lipid - lowering prescription claims at any time & lt;or=12 months before the date of the first set of LDL - C laboratory results . - pubmed
Similar results were observed for subanalyses of subjects with diabetes mellitus or coronary heart disease ( CHD ) ; individuals who received more efficacious statin regimens were more likely to reach the National Cholesterol Education Program Adult Treatment Panel II LDL - C goal of & lt;or=100 mg / dL ( P & lt ; 0.05 for moderate- or low - efficacy regimens vs high - efficacy statins in each model ) . - pubmed
This phenotype , and the fact that this mutation confers resistance to concanamycin , suggests that V - ATPase is the main ( or only ) target for the antibiotics . - pubmed
The role of recombinant lysine - plasminogen and recombinant urokinase and sulfur hexafluoride combination in inducing posterior vitreous detachment . - pubmed
PURPOSE : To determine the optimal method of generating plasmin in vitreous using recombinant lysine - plasminogen and recombinant urokinase and to determine its efficacy in inducing posterior vitreous detachment when combined with sulfur hexafluoride . - pubmed
Intravitreal administration of recombinant lysine - plasminogen and recombinant urokinase did not cause any toxicity findings up to concentrations of 100 microg and 200 IU , respectively , on funduscopy , electroretinography , and histopathologic studies . - pubmed
When combined with sulfur hexafluoride injection , separate intravitreal administrations of 75 microg/0.1 mL of recombinant lysine - plasminogen and 15 IU/0.1 mL of recombinant urokinase induced complete posterior vitreous detachment in 75 % of the eyes compared with 13 % in eyes that received sulfur hexafluoride injection combined with balanced salt solution , recombinant lysine - plasminogen , or recombinant urokinase . - pubmed
CONCLUSIONS : Plasmin was effectively generated in the vitreous after separate intravitreal administrations of recombinant lysine - plasminogen and recombinant urokinase . - pubmed
Intrastriatal administration of the succinate dehydrogenase ( SDH ) inhibitor malonate produces neuronal injury by a " secondary excitotoxic " mechanism involving the generation of reactive oxygen species ( ROS ) . - pubmed
Inhibition of the catabolic enzyme monoamine oxidase ( MAO ) also attenuates striatal lesions induced by malonate . - pubmed
In addition to forming 3,4 - dihydroxyphenylacetic acid , metabolism of dopamine by MAO generates H2O2 , suggesting that dopamine metabolism may be a source of ROS in malonate toxicity . - pubmed
These findings indicate that normal levels of MAO - A participate in expression of malonate toxicity by a mechanism involving oxidative stress . - pubmed
AIM : To investigate the importance of direct contact between Kupffer cells ( KCs ) and hepatocytes ( HCs ) during hepatic inflammatory responses , and the effect of leflunomide 's active metabolite , A(771726 ) , on cytokines in KCs , HCs and KC cocultures ( DC cocultures ) . - pubmed
Leflunomide 's active metabolite , A(771726 ) , had significantly inhibitory effect on TNF - alpha and IL-1 at protein and transcription levels , and the reduced production of IL-1 by A(771726 ) was associated with the inhibitory action of A(771726 ) on TNF - alpha . - pubmed
CONCLUSION : Leflunomide can inhibit hepatocyte damage by inhibiting proinflammatory cytokine release from KCs . - pubmed
Reactive oxygen species - dependent TNF - alpha converting enzyme activation through stimulation of 5 - HT2B and alpha1D autoreceptors in neuronal cells . - pubmed
This was associated with a dose - dependent ( 10 - 40 micromol / L ) upregulation of cytosolic phospholipase A ( 2 ) ( cPLA ( 2 ) ) expression and arachidonic acid release . - pubmed
In contrast , vitamin E dose dependently ( 10 - 60 micromol / L ) inhibited cyclooxygenase ( COX ) activity but did not affect the expression of either COX-1 or COX-2 , indicating that the effect of vitamin E on COX activity was post - translational . - pubmed
BACKGROUND : Sildenafil inhibits phosphodiesterase 5 ( PDE5A ) to elevate intracellular cGMP and to induce vasodilation . - pubmed
In the initial dobutamine test , systolic and diastolic function improved similarly in both treatment groups ( eg , peak power index rose 80+/-28 % in the placebo group and 82+/-31 % in the sildenafil group ; P = NS ) . - pubmed
CONCLUSIONS : PDE5A inhibition by sildenafil blunts systolic responses to beta - adrenergic stimulation . - pubmed
The expression of CA IV , CA VII and CA - RP VIII was somewhat similar : they were expressed in the cortex , hippocampus and midbrain structures and their expression did not change after the kainic acid treatment . - pubmed
Kainic acid stimulated CA XII expression throughout the cortical layer I. - pubmed
Low - temperature Fourier transform infrared ( FTIR ) spectroscopy is used to study squid rhodopsin at 77 K in investigating structural changes in the Schiff base region upon photoisomerization . - pubmed
We report here a novel mechanism for glucose - mediated activation of carbohydrate response element binding protein ( ChREBP ) , a basic helix - loop - helix / leucine zipper ( bHLH / ZIP ) transcription factor of Mondo family that binds to carbohydrate response element in the promoter of some glucose - regulated genes and activates their expression upon glucose stimulation . - pubmed
Structure - function analysis of ChREBP in a highly glucose - sensitive system using GAL4 - ChREBP fusion constructs revealed a glucose - sensing module ( GSM ) that mediates glucose responsiveness of ChREBP . - pubmed
We have identified a new mechanism accounting for glucose responsiveness of ChREBP that involves specific inhibition of the transactivation activity of GRACE by LID under low glucose concentration and reversal of this inhibition by glucose in an orientation - sensitive manner . - pubmed
Chronic fluoxetine treatment increases the expression of PSA - NCAM in the medial prefrontal cortex . - pubmed
As the expression of PSA - NCAM is increased by serotonin in other cerebral regions , antidepressants acting on serotonin reuptake may influence PSA - NCAM expression and thus counteract the effects of depression by modulating neuronal structural plasticity . - pubmed
The effects of fluoxetine treatment for 14 days on mPFC PSA - NCAM expression have also been analyzed . - pubmed
In general , chronic fluoxetine treatment induces significant increases in the number of PSA - NCAM immunoreactive neurons and in neuropil immunostaining and coadministration of the 5 - HT3 antagonist ondansetron blocks the effects of fluoxetine on PSA - NCAM expression . - pubmed
These results indicate that fluoxetine , acting through 5 - HT3 receptors , can modulate PSA - NCAM expression in the mPFC . - pubmed
The peptides were designed to interact electrostatically with siRNA , selecting the TAT peptide domain of HIV ( RKKRRQRRR ) and an oligolysine ( Lys(9 ) ) . - pubmed
In another series of experiments , the geometric mean IC(50)s ( n = 33 ) for fosmidomycin and TH II46 were 413 nM and 249 nM , respectively . - pubmed
FR900098 and TH II46 were 2.6 and 1.7 times more potent , respectively , than fosmidomycin . - pubmed
RESULTS : The women classified as having IR had a significantly higher body mass index ( BMI ) and free androgen index ( FAI ) and showed significantly lower SHBG and high - density lipoprotein ( HDL ) levels , regardless of the indices used . - pubmed
Leptin has been shown to modulate deiodinase type 1 ( D1 ) and type 2 ( D2 ) enzymes responsible for thyroxine ( T4 ) to triiodothyronine ( T3 ) conversion . - pubmed
The AN - HDL treated cells showed an increased production of NO at 3 h versus control - HDL treated cells and a decreased production at 24 h. Regarding LDL , a significant increase of NO production was obtained both at 3 and 24 h. The AN - HDL and AN - LDL treated cells showed an increased production of peroxynitrite both at 3 and 24 h compared to control lipoproteins . - pubmed
Densitometric analysis of bands indicated that both iNOS and nNOS protein levels were significantly higher in the cells incubated with AN lipoproteins compared to cells incubated with control lipoproteins both at 3 and 24 h. Although the pathogenesis of AN remains uncertain , evidence exists that modifications to the lipoprotein profile and cholesterol , structural alterations of phospholipids and integral constituents of myelin and synaptosomes may be related to psychotic disorders and body image distortion common to AN . - pubmed
The multivariate analysis for biochemical relapse - free survival revealed that prostate - specific antigen ( relative risk , 1.002 ; 95 % CI , 1.001 - 1.003 ; P = 0.0041 ) , Gleason score ( relative risk , 1.166 ; 95 % CI , 1.046 - 1.302 ; P = 0.0055 ) , T classification ( relative risk , 2.897 ; 95 % CI , 1.999 - 4.230 ; P = 0.0000 ) , pelvic irradiation ( relative risk , 2.042 ; 95 % CI , 1.328 - 3.273 ; P = 0.0008 ) , and androgen abletion ( relative risk , 0.321 ; 95 % CI , 0.240 - 0.427 ; P = 0.0000 ) were significant prognostic factors . - pubmed
Physical nature of intermolecular interactions within cAMP - dependent protein kinase active site : differential transition state stabilization in phosphoryl transfer reaction . - pubmed
Differential transition state stabilization ( DTSS ) methodology has been applied herein to the phosphoryl transfer reaction catalyzed by cAMP - dependent protein kinase ( PKA ) . - pubmed
The MP2 results correlate well with the available experimental data and theoretical findings indicating that Lys72 , Asp166 , and the two magnesium ions contribute -22.7 , -13.3 , -32.4 , and -15.2 kcal / mol to differential transition state stabilization , respectively . - pubmed
Using scanning tunneling microscopy ( STM ) and density functional theory simulations , we have studied the diffusion of alkoxy species formed by the dissociation of alcohols on bridge - bonded oxygen ( BBO ) vacancies ( BBO ( V ) 's ) on TiO2 ( 110 ) . - pubmed
At elevated temperatures ( & gt;or=400 K ) the sequential isothermal STM images show that mobile BBO ( V ) 's mediate the diffusion of alkoxy species by providing space for alkyl - group - bearing BBO atom to diffuse into . - pubmed
TEM images and thermogravimetric analysis reveal that the microemulsion interface and the PEG400 agent have a synergistic effect on the morphology and crystalline size transition of ZnO nanostructures . - pubmed
Immersion experiments in a simulated body fluid ( SBF ) were carried out to examine the Ca - P phase forming ability of the modified surfaces . - pubmed
Ectopic expression of beta - catenin also decreased the expression of GLI1 in the butyrate treated cancer cells . - pubmed
The peak temperature of the temperature - programmed desorption profiles of toluene from the CC microspheres was higher than that from granular activated carbon ( GAC ) with numerous micropores , indicating that toluene is adsorbed more strongly on CC microspheres than on GAC . - pubmed
P2Y12 receptor - mediated integrin - beta1 activation regulates microglial process extension induced by ATP . - pubmed
Microglia rapidly extend their processes toward the damaged areas in response to stimulation of the metabotropic ATP receptor P2Y ( 12 ) by ATP released from damaged tissue . - pubmed
In this study , we showed that ATP increased microglial adhesion to collagen gel , and that the ATP - induced process extension and increase in microglial adhesion were inhibited by integrin blocking peptides , RGD , and a functional blocking antibody against integrin - beta1 . - pubmed
An immunoprecipitation analysis with an antibody against the active form of integrin - beta1 showed that P2Y ( 12 ) mediated the integrin - beta1 activation by ATP . - pubmed
These findings indicate that ATP induces the integrin - beta1 activation in microglia through P2Y ( 12 ) and suggest that the integrin - beta1 activation is involved in the directional process extension by microglia in brain tissue . - pubmed
The decapeptide gonadotropin - releasing hormone ( GnRH ) is the primary factor responsible for the hypothalamic control of gonadotropin secretion . - pubmed
GnIH acts on the pituitary and on GnRH neurons in the hypothalamus via a novel G protein - coupled receptor ( GPR147 ) . - pubmed
GnIH decreases gonadotropin synthesis and release , inhibiting gonadal development and maintenance . - pubmed
Melatonin stimulates the expression and release of GnIH via melatonin receptors expressed by GnIH neurons . - pubmed
Data indicate that serine / threonine protein kinase Akt ( Akt ) signaling cooperates with Wingless ( Wnt ) to activate beta - catenin in intestinal stem and progenitor cells through phosphorylation at Ser552 ( P - beta - catenin(552 ) ) . - pubmed
The midpoint cytotoxicity values for a 24 - hr exposure to dichlone was about 0.02 mm when evaluated with the neutral red , acid phosphatase , and XTT tetrazolium assays . - pubmed
Lactic acid dehydrogenase leakage , after a 4 - hr exposure , occurred initially at 0.05 mm dichlone . - pubmed
We first identified that HLA - B*1502 is strongly associated with carbamazepine ( CBZ)-induced SJS / TEN and HLA - B*5801 with allopurinol - SJS / TEN in Han Chinese . - pubmed
We used MG-132 treatment of HBE cells as an in vitro model to standardize the efficacy of salubrinal ( inhibitor of eIF2alpha de - phosphorylation ) in controlling the accumulation of ubiquitinated proteins and the NFkappaB levels . - pubmed
Moreover , our data suggest the therapeutic efficacy of salubrinal in restraining NFkappaB mediated inflammation in the adult or older subjects . - pubmed
The elevated cdc2 had inactivating phosphorylation on Thr(14 ) or Tyr(15 ) , and possibly both , due to an increase in the responsible Wee1 and Myt1 kinases . - pubmed
In cells that harbored cytoplasmic cyclin B1 or cdc2 , there was also an accumulation of the phosphatase - inactive cdc25C phosphorylated on Ser(216 ) , unable to activate cdc2 . - pubmed
Moreover , E7 expression induced elevated expression of phosphorylated ATM ( Ser(1981 ) ) and the downstream phosphorylated Chk1 , Chk2 , and JNKs , kinases known to inactivate cdc25C. - pubmed
A single percutaneous IT injection of BoNT - B 0.5 U at 2 or 5 days prior to IPLT formalin reduced NK1 - R internalization and C - Fos expression . - pubmed
IT BoNT - B also produced a corresponding reduction in phase 2 of formalin - evoked flinching behavior for over 30 days after IT injection . - pubmed
Romidepsin : a novel histone deacetylase inhibitor for cancer . - pubmed
INTRODUCTION : Romidepsin is a novel histone deacetylase ( HDAC ) inhibitor , with a recent approval for treatment of cutaneous T - cell lymphoma ( CTCL ) . - pubmed
In addition , the Phase II trial in CTCL leading to FDA approval of romidepsin is reviewed in detail . - pubmed
Literature search was performed using PubMed ; keywords and concepts used included romidepsin , T - cell lymphoma and HDAC inhibitors . - pubmed
EXPERT OPINION : Romidepsin is a potent HDAC inhibitor with demonstrable activity in T - cell lymphoma . - pubmed
PURPOSE : Knowing the glycolytic phenotype of cancers is important for the appropriate use of F-18 FDG PET / CT imaging . - pubmed
The F-18 FDG PET / CT uptake was determined as maximum standard uptake value ( SUVmax ) at the pathologically confirmed site of disease or in the most active lesion . - pubmed
SmCI is a 165 - residue glycoprotein with a molecular mass of 19.69 kDa ( mass spectrometry ) and 18 cysteine residues forming nine disulfide bonds . - pubmed
SmCI is the first proteinaceous inhibitor of metallocarboxypeptidases able to act as well on another mechanistic class of proteases ( serine - type ) and is the first of this kind identified in nature . - pubmed
These PAHs changed the studied gene transcriptions differently , but not significantly , except for CYP1A , which was induced only by RET . - pubmed
MRSA cell counts on the surface of the non - film - coated control sheet were high ( 6,600 - 11,000 colony forming units ( cfu ) ) , but those on the copper film were considerably lower ( 20 - 130 cfu ) . - pubmed
Use of metallic copper on the bed sheets of patients who are likely to be a source of MRSA contamination may help to prevent the spread of MRSA contamination in hospital wards . - pubmed
We analyzed the amounts of some noteworthy proteins in these two samples including lectin , superoxide dismutase , glycoside hydrolase and proteins involved in cordycepin metabolism , providing important information for further protein studies . - pubmed
This study investigated the extent of carbon capture in a wet - dumped ash dam and the mineralogical changes promoting CO2 capture , comparing this natural phenomenon with accelerated ex - situ mineral carbonation of fresh fly ash ( FA ) . - pubmed
In view of the predicted frequencies and relative clinical significance of both private antigens and high - prevalence antigens absent , the most relevant assays for individuals of African descent in a transfusion setting are for 1 ) partial RH2 in the patient and 2 ) RH54 ( DAK ) in the donor . - pubmed
Experimental measurements indicate that , when compared to the commonly known face - centered cubic ( 3C ) form of silver , the 4H - Ag form shows a 130 - fold higher , strongly anisotropic , in - plane resistivity and a much lower optical reflectance with a pronounced surface plasmon contribution that imparts a distinctive golden hue to the material . - pubmed
The pressure swing adsorption ( PSA ) , physical absorption ( Selexol ) , and chemical looping combustion ( CLC ) technologies were separately analyzed for processes with CO2 capture . - pubmed
The results show that , when only electricity production in considered , the case using CLC technology has an electrical efficiency 1.3 % and 2.3 % higher than the PSA and Selexol based cases , respectively . - pubmed
The CLC based process achieves an overall CO2 capture efficiency of 99.9 % in contrast to 89.9 % for PSA and 93.5 % for Selexol based processes . - pubmed
The overall efficiency of the CLC case for combined electricity and H2 production is marginally higher ( by 0.3 % ) than Selexol and lower ( by 0.6 % ) than PSA cases . - pubmed
These plasmanylinositols respond acutely to stimulation of cells with chemoattractants , and their levels are regulated by PIPKs , PI3Ks and PTEN . - pubmed
( R )- 3 - amino-1 -(( 3aS,7aS )- octahydro-1H - indol-1 - yl )- 4 -( 2,4,5 - trifluorophenyl ) butan-1 - one derivatives as potent inhibitors of dipeptidyl peptidase-4 : design , synthesis , biological evaluation , and molecular modeling . - pubmed
A series of ( R )- 3 - amino-1 -(( 3aS,7aS )- octahydro-1H - indol-1 - yl )- 4 -( 2,4,5 - trifluorophenyl ) butan-1 - one derivatives was designed , synthesized , and evaluated as novel inhibitors of dipeptidyl peptidase-4 ( DPP-4 ) for the treatment of type 2 diabetes . - pubmed
Thus , a highly sensitive and selective ECL probe for the recognition of Co ( 2 + ) was developed with a stable response over a concentration range of 0.9 muM to 6.3 muM and a detection limit as low as 21 nM. - pubmed
Moreover , the taxonomic analysis based on high through - put 16S rRNA gene sequencing revealed that the high abundance of denitrifying bacteria and nitrite - oxidizing bacteria ( NOB ) was responsible for low nitrite accumulations and consequent low N2O emissions . - pubmed
The meta - analysis also revealed decreased CK - MB , cTnI and MDA , and increased content of SOD . - pubmed
Vpac2a is activated exclusively by peptide histidine isoleucine ( PHI ) , which suggests that Pac1a mediates the melanin aggregating effect of PACAP on melanophores . - pubmed
Efficacy of Lapatinib in Therapy - Resistant HER2 - Positive Circulating Tumor Cells in Metastatic Breast Cancer . - pubmed
BACKGROUND : To evaluate the efficacy of lapatinib , a dual EGFR and HER2 tyrosine kinase inhibitor , in therapy - resistant HER2 - positive CTCs in metastatic breast cancer ( MBC ) . - pubmed
Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells : down - regulation of carboxylesterase 1 , drug sequestration in lipid droplets and pro - survival activation ERK / Akt / mTOR . - pubmed
Here we identified the molecular basis of acquired resistance to CHR2863 , an orally available hydrophobic aminopeptidase inhibitor prodrug with an esterase - sensitive motif , in myeloid leukemia cells . - pubmed
CHR2863 enters cells by diffusion and is retained therein upon esterase activity - mediated conversion to its hydrophilic active metabolite drug CHR6768 , thereby exerting amino acid depletion . - pubmed
Remarkably , the latter feature coincided with a gain of sensitivity to the mTOR inhibitor rapamycin . - pubmed
The electronic structures show that the large Li adsorption energy and fast diffusion ability of the P / G origin from the interfacial synergy effect . - pubmed
The scaffolding and hub protein postsynaptic density protein-95 ( PSD-95 ) is a major element of central chemical synapses and interacts with glutamate receptors , cell adhesion molecules , and cytoskeletal elements . - pubmed
Pharmacological activities are mainly mediated via opioid receptors as well as neuronal Ca2 + channels , expression of cAMP and CREB protein and via descending monoaminergic system . - pubmed
Osimertinib ( AZD9291 ) , a Mutant - Selective EGFR Inhibitor , Reverses ABCB1 - Mediated Drug Resistance in Cancer Cells . - pubmed
In this study , we determine whether osimertinib , a novel selective , irreversible EGFR ( epidermal growth factor receptor ) TKI , could reverse ABC transporter - mediated MDR . - pubmed
The results showed that , at non - toxic concentrations , osimertinib significantly sensitized both ABCB1 - transfected and drug - selected cell lines to substrate anticancer drugs colchicine , paclitaxel , and vincristine . - pubmed
Osimertinib significantly increased the accumulation of [ 3H ]- paclitaxel in ABCB1 overexpressing cells by blocking the efflux function of ABCB1 transporter . - pubmed
In contrast , no significant alteration in the expression levels and localization pattern of ABCB1 was observed when ABCB1 overexpressing cells were exposed to 0.3 microM osimertinib for 72 h. In addition , ATPase assay showed osimertinib stimulated ABCB1 ATPase activity . - pubmed
Taken together , our findings suggest that osimertinib , a clinically - approved third - generation EGFR TKI , can reverse ABCB1 - mediated MDR , which supports the combination therapy with osimertinib and ABCB1 substrates may potentially be a novel therapeutic stategy in ABCB1 - positive drug resistant cancers . - pubmed
Hemodilution was produced by mixing the blood with 40 % Tris / saline solution , fibrinolysis by treating the blood with 160 ng / mL tPA , acidosis by adding 1.2 mg / mL lactic acid achieving pH 7.0 to 7.1 , and hypothermia by running the assay at 31 C. Intact blood tested at 37 C served as control . - pubmed
In 2014 ( 2015 , 2016 ) , 7 % ( 14 % , 19 % ) , 17 % ( 27 % , 34 % ) , 51 % ( 67 % , 70 % ) and 88 % ( 97 % , 98 % ) of the population in China lived in areas that meet the level of annual PM2.5 , PM10 , NO2 , and SO2 standard metrics from Chinese Ambient Air Quality Standards - Grade II . - pubmed
Here , we characterized the liver macrophage subsets during methionine / choline deficient ( MCD ) diet - induced NASH and recovery . - pubmed
IL - MG was synthesized by a simple and time - saving one - pot strategy where the synthesis of magnetic Fe3O4 , the modification with an IL , and the reduction of graphene oxide to graphene were conducted at the same time . - pubmed
Moreover , 1064 AG patients had significantly higher frequencies of microcytosis , macrocytosis , hemoglobin , iron , vitamin B12 , and folic acid deficiencies , hyperhomocysteinemia , and serum GPCA positivity than 532 healthy control subjects ( all P - values & lt ; 0.005 ) . - pubmed
OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate . - pubmed
Auxin , nitric oxide ( NO ) , and strigolactones ( SLs ) are important regulators of root growth under low - nitrogen and -phosphate ( LN and LP ) conditions . - pubmed
qRT - PCR analysis of OsPIN family genes showed that the expression of OsPIN1b is most abundant in root tip and is significantly downregulated by LN , LP , sodium nitroprusside ( SNP , NO donor ) , and GR24 ( analogue of SLs ) treatments . - pubmed
Seminal roots in ospin1b mutants were shorter than those of the wild type ; and the seminal root , [ 3H ] IAA transport , and IAA concentration responses to LN , LP , SNP , and GR24 application were attenuated in ospin1b-1 mutants . - pubmed
Between baseline and month 6 , mean serum bicarbonate increased from 23.1 to 24.1 mmol / L ( P = 0.01 ) , mean serum albumin increased from 36.8 to 37.8 g / L ( P = 0.03 ) , mean serum C - reactive protein increased from 7.3 to 12.4 mg / L ( P = 0.05 ) , and mean serum potassium decreased from 4.80 to 4.59 mmol / L ( P = 0.01 ) . - pubmed
Despite showing clinical activity in BRAF - mutant melanoma , the MEK inhibitor ( MEKi ) trametinib has failed to show clinical benefit in KRAS - mutant colorectal cancer . - pubmed
The bromodomain inhibitor JQ1 suppressed interferon - stimulated gene ( ISG ) expression and in combination with MEK inhibitors displayed synergistic effects and induced apoptosis in MEKi - resistant colorectal cancer cell lines . - pubmed
ISG expression was confirmed in patient - derived organoid models , which displayed resistance to trametinib and were resensitized by JQ1 co - treatment . - pubmed
Since the PGE2 receptor has four subtypes , EP1 - EP4 , this study was aimed to challenge the hypothesis that at least one of the four subtypes is responsible for the pathogenesis of dioxin - induced hydronephrosis . - pubmed
To expose the pups to 2,3,7,8 - tetrachlorodibenzo - p - dioxin ( TCDD ) via lactation , the dams were administered TCDD at an oral dose of 20 mug / kg on PND 1 . - pubmed
We found that the incidence of hydronephrosis was 80 % in the EP1+/+ group , but was markedly reduced to 28.6 % in the EP1-/- group despite the fact that PGE2 concentration in the urine was similarly increased in the both groups . - pubmed
With regard to EP4 , the incidence of hydronephrosis in vehicle ( saline)-treated groups and ONO - treated was 88.9 % and 100 % , respectively . - pubmed
Tyrosinase based amperometric biosensor for determination of tyramine in fermented food and beverages with gold nanoparticle doped poly ( 8 - anilino-1 - naphthalene sulphonic acid ) modified electrode . - pubmed
The biosensor architecture is based on tyrosinase ( Tyrase ) immobilization on glassy carbon electrode modified by a nanocomposite consisting of gold nanoparticles ( AuNP ) synthesized by a green method and poly ( 8 - anilino-1 - naphthalene sulphonic acid ) modified glassy carbon electrode . - pubmed
OBJECTIVES : To determine the proportion of rifampicin - resistant tuberculosis ( RR - TB ) patients who accessed second - line drug susceptibility testing ( SL - DST ) results following introduction of the Hain technology in southern provinces , Zimbabwe . - pubmed
We , for the first time , prospectively evaluated the healed status in TB spine by fluorodeoxyglucose - positron emission tomography / computed tomography ( FDG - PET / CT ) and contrast magnetic resonance imaging ( MRI ) with the objective to define end - point of treatment in TB spine . - pubmed
ATT was stopped on contrast MRI - based healing or absence of FDG uptake on PET - CT . - pubmed
FDG - PET / CT showed healed bone lesion in 28/28 ( 100 % ) and on MRI 13/19 ( 68.42 % ) , respectively . - pubmed
Conclusions : FDG - PET / CT is more useful to demonstrate the healed status than MRI and is the only imaging to demonstrate healed status when MRI could not be performed due to metallic implants . - pubmed
Oxylipins include hundreds of structurally and stereochemically distinct lipid species which today are most commonly analyzed by ( ultra ) high performance liquid chromatography - mass spectrometry based ( ( U)HPLC - MS ) methods . - pubmed
The meta - analysis showed significantly better fasting blood glucose and hemoglobin A1c ( HbA1c ) level . - pubmed
In secondary analyses , there was a remarkable reduction in total cholesterol and low - density lipoprotein postintervention . - pubmed
IMPACT : Existing reviews are limited to narrative synthesis , lacking critical appraisal , heterogenous outcomes , nor any report of fasting blood glucose and HbA1c . - pubmed
Then , three electrodes of ITO / VO2(M)/Au were prepared : electrode ( I ) , electrode ( II ) , and electrode ( III ) at annealing temperatures of 550 , 650 , and 750 C , respectively . - pubmed
Independent predictors of PMV included age , intraoperative maximum vasoactive - inotropic score , minimal temperature during cardiopulmonary bypass , postoperative prothrombin time , alkaline phosphatase and total bilirubin levels , and postoperative fluid balance . - pubmed
CONCLUSIONS : Age at surgery , maximal vasoactive - inotropic score and minimal temperature during cardiopulmonary bypass , postoperative prothrombin time , alkaline phosphatase and total bilirubin levels , and postoperative fluid balance were demonstrated to be independent predictors of PMV . - pubmed
Accordingly , IM - MS experiments performed in Ag+-doped methanol revealed the presence of both ( + ) - and ( -)- alpha - bisabolol in the EO , while molecular simulations provided the structures of the two alpha - bisabolol enantiomer silver ( I ) adducts . - pubmed
Methods : Two adult patients with type 1 diabetes ( T1D ): one new onset and the other one with established T1D are presented where telehealth facilitated by Clarity Software and the " Share " feature with the use of Dexcom G6 continuous glucose monitoring ( CGM ) for management of diabetic ketosis and hyperglycemia . - pubmed
Shared glucose data through CGM facilitated frequent insulin dose adjustments , increased fluid and carbohydrate intake , and prevented hospital admissions in both cases . - pubmed
The reduction in the gene expression levels of Opa1 and Drp1 was also reversed by MA-5 , suggesting the modification of the fusion / fission process . - pubmed
At the end of 60 days on these diets , blood glucose , total blood cholesterol , triglycerides ( TGs ) , and high density lipoprotein ( HDL ) were quantitated . - pubmed
MATERIALS AND METHODS : The cytotoxic activity of the compound ( at 0.1 - 20.0 mug / mL ) on the cells was determined by Real - Time Cell Analysis ( RTCA ) system for 24 h. The estimation of cell cycle arrest , reactive oxygen species ( ROS ) production , reduction of mitochondrial membrane potential ( MMP ) , and caspases-3/7/9 activity in the HeLa cells treated with the compound was done by flow cytometry and luminometric technique . - pubmed
Demographic data and imaging data of fundus photography , fundus autofluorescence ( FAF ) , confocal blue - light reflectance ( CBR ) , fluorescein angiography ( FAG ) , and optical coherence tomography ( OCT ) were collected . - pubmed
ATPase activity of cardiac myosin was stimulated as the Ca2 + concentration in the incubation medium increased . - pubmed
No difference was found in Ca2 + -activation , salt sensitivity or ATPase activity of unreacted and sulphydrylmodified cardiac myosins from euthyroid or hyperthyroid groups . - pubmed
The TCN was also phosphorylated in a cellfree extract containing adenosine kinase activity at about the same rate as was adenosine , but not further phosphorylation of the analogue MP occurred . - pubmed
Added protein reduced the effectiveness of Cu ( II ) as a catalyst of the reaction between ascorbate and MNNG , which suggested that the microsomal protein necessary to activate DMN , may reduce the concentration of free Cu ( II ) and thereby lower its catalytic efficiency . - pubmed
Serum Ca was 1.35 mmol / l , P = 0.99 mmol / l with high alkaline phosphatases ( A.P. ) - pubmed
Serum Cu was low = 3.14 mumol / l and serum immunoreactive parathormone ( iPTH ) level was elevated : 520 mulEq / ml ( N less than or equal to 100 ) . - pubmed
Administration of vitamin D2 ( 15 mg ) induced an immediate normalization of serum Ca , normal serum iPTH ( 68 mulEq / ml ) in one month , normal X - rays in two months and normal A.P. - pubmed
We have made optical detection of magnetic resonance ( ODMR ) measurements on the enzyme alpha - chymotrypsin , as well as on its complex with the dye , proflavin . - pubmed
The luminescence decay kinetics of the complex indicates that nontrivial triplet - singlet transfer originates from several ( at least three ) tryptophan residues of the enzyme . - pubmed
The method is very simple as sample preparation is minimal , and the isocratic elution of the C18 column with pure methanol does not necessitate a sophisticated instrumental set - up . - pubmed
cruzi causes in the expression of TcR , CD4 , CD8 and IL-2R -- all molecules playing important roles in lymphocyte activation -- could contribute to the development of the immunosuppression observed during the acute phase of Chagas ' disease . - pubmed
All of them are non - enzymatically induced by 0.1 mM calcium ion , which is the ultimate concentration of sarcoplasmic calcium ion in post mortem muscles . - pubmed
Transnasal butorphanol : a new method for pain relief in post - cesarean section pain . - pubmed
It is concluded that transnasal butorphanol represents a safe and effective alternative to injectable butorphanol for post - cesarean section pain and offers a better and longer duration of analgesia compared to IV butorphanol . - pubmed
The optimum dose seems to be 2 mg TN butorphanol and it is tolerated better when divided into 1 mg increments , given 1 h apart . - pubmed
The effect of ATP is apparently mediated by the activation of cytosolic protein kinases and requires the influx of Ca2 + through the plasma membrane . - pubmed
All sera were tested for total immunoglobulin ( Ig ) levels ; IgG , IgM , and IgA isotypes of autoantibodies to six phospholipids ; total histone and four histone subfractions ; and four polynucleotides . - pubmed
Only a few autoantibodies were increased in early pregnancy , including IgG antiphosphatidylinositol and H 2B , IgM antiphosphatidylinositol and phosphatidic acid , and IgA antiphosphatidylinositol and H 4 . - pubmed
Amino acid substitutions are nonrandomly distributed throughout the apolipoprotein A - I primary structure . - pubmed
These substitutions are the result of transversions of cytosines contained within stretches of at least 5 consecutive cytosines in the apoA - I gene . - pubmed
The observed hypervariability of the apoA - I amino terminus , therefore , might be caused by a hot spot for mutation formed by the 7 subsequent cytosines in codons 3 , 4 , and 5 . - pubmed
The hypervariability of the apoA - I alpha - helical domain might therefore be caused by CpG dinucleotides predominantly occurring in codons 120 - 208 of apoA - I ( 82 out of 125 ) . - pubmed
The isosteric CH ---- N substitution in the class of 2 - arylpenems results in improved antibacterial activity , with retention of the favorable characteristic of stability towards renal dehydropeptidase . - pubmed
The aggregational state and conformation of the protein were affected by ( 1 ) the method of coat protein isolation ( phenol extraction vs cholate isolation ) , ( 2 ) the nature of amphiphiles used ( variation in phospholipid headgroups and acyl chains ) , and ( 3 ) the ratio of amphiphiles and protein . - pubmed
Under all conditions , phenol - extracted coat protein was in a predominantly beta - structure and in a highly aggregated polymeric form . - pubmed
Cholate - isolated coat protein was initially oligomeric and contained a substantial amount of alpha - helix . - pubmed
This effect was observed upon uptake in phospholipids at low lipid to protein molar ratios ( L / P ratios ) and with phosphatidylcholines ( PC ) and phosphatidic acids ( PA ) containing saturated acyl chains . - pubmed
After reconstitution in phospholipids with unsaturated acyl chains and with phosphatidylglycerols ( PG ) at high L / P ratios , the original alpha - helix - containing state of the coat protein was maintained . - pubmed
Ontogenetic changes in the cyclic adenosine 3 ' , 5 '- monophosphate - stimulatable phosphorylation of cat visual cortex proteins , particularly of microtubule - associated protein 2 ( MAP 2 ): effects of normal and dark rearing and of the exposure to light . - pubmed
In vitro phosphorylation was assayed with and without cAMP and the cAMP - dependent protein kinase , and the phosphoproteins separated by sodium dodecyl sulfate - polyacrylamide gel electrophoresis were counted for 32P incorporated from [ gamma-32P ] ATP . - pubmed
By contrast , the cAMP - stimulatable phosphorylation of microtubule - associated protein 2 ( MAP 2 ) , which under normal rearing conditions increases from birth to the second month , is strongly modulated by the presence of light in the environment . - pubmed
The cAMP - dependent phosphorylation of MAP 2 ( and its dephosphorylation ) may be an important factor for determining the state of plasticity in the CP through its affecting the dendritic cytoskeletal organization involving tubulin and actin . - pubmed
Needle biopsy evaluation of class II major histocompatibility complex antigen expression for the differential diagnosis of cyclosporine nephrotoxicity from kidney graft rejection . - pubmed
2 - Deoxyglucose uptake was about 95 % depressed in insulin - treated , but not in ' basal ' , cells preincubated with epinephrine plus adenosine deaminase for 60 min in broad agreement with Green 's report . - pubmed
In similarly incubated cells , transport of 3 - O - methylglucose , a sugar analogue not phosphorylated in the adipocytes , was not affected by catecholamine plus adenosine deaminase . - pubmed
However , a decrease in transport of about 60 % was observed both in the absence and the presence of insulin when the albumin concentration was high enough and the cell concentration low enough to prevent accumulation of free fatty acids in the medium . - pubmed
In addition , the insulin sensitivity with regard to hexose transport was markedly reduced . - pubmed
Transport was approximately doubled in cells incubated with 5 microM epinephrine in the absence of adenosine deaminase . - pubmed
Thus , epinephrine at a high concentration stimulates hexose transport in the absence of adenosine deaminase ( presence of adenosine ) whereas it inhibits both basal and insulin - stimulated transport in the presence of adenosine deaminase ( absence of adenosine ) . - pubmed
Serial prolactin growth hormone , insulin , blood sugar , cortisol , glucagon , thyrotropin stimulating hormone , thyroxine , and triiodothyronine were obtained by RIA . - pubmed
Requirement of substrate for adenosine 5 '- monophosphate binding to liver fructose 1,6 - diphosphatase . - pubmed
Breakage of DNA in rec+ and Rec- bacteria by disintegration of radiophosphorus atoms in DNA and possible cause of pleiotropic effects of RecA mutation . - pubmed
Glutamine showed increased rates of uptake to the sensorimotor cortex during stimulation periods , suggesting an accelerated breakdown via glutaminase . - pubmed
Biochemical studies showed only trivial increases in levels of glutamic - pyruvic transaminase ( GPT ) , glutamic - oxalacetic transaminase ( GOT ) , and moderate but temporary increases in isocitrate dehydrogenase ( ICD ) after CCl4 alone . - pubmed
[ LH - RH agonist buserelin ] . - pubmed
The regulation of acetoacetyl - CoA synthetase activity by modulators of cholesterol synthesis in vivo and the utilization of acetoacetate for cholesterogenesis . - pubmed
Acetoacetyl - CoA synthetase activity was depressed by feeding cholesterol or mevalonate administration and was enhanced by the addition to the diet of mevinolin and/or cholestyramine . - pubmed
The evidence suggests that the regulation of acetoacetyl - CoA synthetase activity and the utilization of acetoacetate for lipogenesis is closely linked to the regulation of cholesterogenesis . - pubmed
Turnover of low density lipoproteins during inhibition of cholesterol absorption by neomycin . - pubmed
During treatment with neomycin , the plasma total cholesterol fell by an average of 20 % , low density lipoproteins ( LDL ) fell by 25 % , and high density lipoproteins , by 16 % . - pubmed
Neomycin decreased synthesis of apoLDL by 28 % . - pubmed
Differences between HbA and Chromatium high - spin hemoprotein spectra can be assigned to either the effects of a covalent linkage of the heme vinyls to the protein matrix or alterations in the heme - proximal ligand bonding interaction . - pubmed
The behavior of heme core - size sensitive modes and low - frequency modes in Chromatium high - spin hemoprotein may be an indication of distortions in the heme geometry of Chromatium high - spin hemoprotein relative to HbA. - pubmed
Re - chromatography of CWI peak fractions on SPS using NaCl - gradient elution showed the presence of a 17 - kDa peptide ( p17 ) in fractions with low CWI activity but strong CWI immunosignal ( Weil and Rausch 1994 , Planta 193 , 430 - 437 ) . - pubmed
When separating CWI from p17 by Concanavalin A ( Con A)-Sepharose chromatography , inhibition could be restored by incubating the inhibitor - containing fraction with inhibitor - free CWI . - pubmed
The presence of divalent metal ions ( Ca2 + , Mg2 + , Zn2 + ) during pre - incubation of CWI with p17 reduced CWI inhibition substantially . - pubmed
Also , sucrose protected CWI against inhibition by p17 ( half - maximum protection at 1.3 mM ) . - pubmed
When fractions containing CWI and p17 were analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( SDS - PAGE ) and subsequent Western blotting a diffuse immunosignal of 86 - 90 kDa was observed ( in addition to the prominent CWI signal at 69 kDa). - pubmed
The pyridylcyanoguanidine pinacidil exerts its hypotensive effect by opening ATP - sensitive potassium channels ( K+ATP ) in vascular smooth muscle . - pubmed
Methylation analysis and 1 and 2D NMR spectroscopy showed that the K10 capsular polysaccharide has the structure [ formula : see text ] - pubmed
Treatment of the cells with L - DOPS increased the NGF content in the L - M cell medium by approximately 3 - fold . - pubmed
The antioxidants , ascorbic acid and sodium pyrosulfite , completely prevented the stimulatory effect of L - DOPS , and radical scavengers ( superoxide dismutase plus catalase ) caused a significant partial inhibition of the response to L - DOPS . - pubmed
Quinone derivatives ( adrenochrome , 4 - n - propyl-1,2 - benzoquinone ) , which are the oxidative products of the catechol derivatives , increased the NGF content in the medium , and their potency was greater than that of the catechol derivatives themselves . - pubmed
However , PMA significantly abolished these effects of SSb2 in including proliferation inhibition and apoptosis induction . - pubmed
Down - regulation of the PKC activity may be involved in the effect of SSb2 . - pubmed
The proportion of sperm with active acrosin were determined using the biotinylated isocoumarin serine protease inhibitor , Bi - Aca - Aca - OMe - IC ( BIC ) . - pubmed
The presence of bound inhibitor on sperm was then determined by secondary labeling with avidin fluorescein conjugate . - pubmed
Histamine induced significant MGP secretion from both explants and isolated glands . - pubmed
The inhibitory action of SKF 91488 in airway explants was blocked by indomethacin and was significantly reduced by a prostanoid EP4 receptor antagonist ( AH23848B ) . - pubmed
Similar calcium , conductance , and capacitance responses could be activated by 2 - methylthio - ATP and ADP , consistent with mediation by a P2 type purinergic receptor . - pubmed
These myriad responses to extracellular ATP suggest that P2 receptor - mediated signaling is important in brown adipocyte physiology and that sympathetic stimulation may normally activate purinergic as well as adrenergic pathways in brown fat . - pubmed
Using a DIG - labeled aMAG antisense RNA as a probe , we have investigated the developmental and tissue - specific expression of this repair gene . - pubmed
Antral biopsy samples were taken for hematoxylin - eosin stain ( HE ) , rapid urease test , and culture and corpus samples for culture and HE . - pubmed
This leader presents striking similarity to ' T - box ' leaders found upstream of many Gram - positive aminoacyl - tRNA synthetase genes and some amino acid biosynthesis operons , which are controlled by antitermination through interaction of the leader transcript with cognate uncharged tRNA . - pubmed
Surprisingly , RAD52(-/- ) cells were not hypersensitive to DNA damages induced by gamma - irradiation , methyl methanesulfonate , or cis - platinum ( II ) diammine dichloride ( cisplatin ) . - pubmed
A comparative SAR study of thrombin receptor derived non peptide mimetics : importance of phenyl / guanidino proximity for activity . - pubmed
Synthetic thrombin receptor peptides ( TRAPs ) comprising the first 5 amino acids ( SFLLR and SFLLR - NH2 ) of the new N - terminus tethered ligand of the thrombin receptor that is generated by thrombin 's proteolytic activity were found to cause full platelet aggregation . - pubmed
During the screening of novel thrombin receptor derived non - peptide mimetics in the platelet aggregation assay we found that 1 - phenylacetyl-4 -( 6 - guanidohexanoyl )- piperazine ( 1 ) and 1 -( 6 - guanidohexanoyl )- 4 -( phenylacetylamidomethyl )- piperidine ( 2 ) exerted in vitro antagonist activities ( 56 % and 40 % correspondingly ) as it is depicted by the platelet aggregation assay . - pubmed
Correlation between polymerase chain reaction analysis of the histidine biosynthesis operon , randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates . - pubmed
The O - oxime derivatives from aldehyde reaction with PFBHA were desorbed with hexane , and quantified by capillary gas chromatography / mass spectrometry ( GC / MS ) or gas chromatography / electron capture detection ( GC / ECD ) . - pubmed
Other data on valeraldehyde and acrolein have shown that the sampling constants were independent of face velocity between 0.1 to 0.35 m / s ( 20 to 70 fpm ) , temperatures between 9 to 48 degrees C , RH between 3 to 79 percent , and intermittent sampling exposure pattern . - pubmed
In all cases , the cells were present in nodal sinuses and appeared as single cells or small clusters . - pubmed
Parathion induced significant depression of both AChE and LDH activities . - pubmed
No significant assay interference was caused by a high concentration of triglyceride , hemoglobin , bilirubin , uric acid , or creatinine . - pubmed
Neuronal apoptosis induced by pharmacological concentrations of 3 - hydroxykynurenine : characterization and protection by dantrolene and Bcl-2 overexpression . - pubmed
In cerebellar granule cells , the 3 - HK neurotoxicity was unaffected by xanthine oxidase inhibitors but markedly potentiated by superoxide dismutase and its hemelike mimetic , MnTBAP [ manganese ( III ) tetrakis ( benzoic acid ) porphyrin chloride ] . - pubmed
Catalase blocked 3 - HK neurotoxicity in the absence and presence of superoxide dismutase or MnTBAP . - pubmed
Taken together , our results suggest that neurotoxicity induced by pharmacological concentrations of 3 - HK in these cell types is mediated primarily by H ( 2 ) O ( 2 ) , which is formed most likely by auto - oxidation of 3 - HK in extracellular compartments . - pubmed
The protection by dantrolene was associated with a marked increase in the protein level of Bcl-2 , a prominent antiapoptotic gene product . - pubmed
Thus , dantrolene may elicit its neuroprotective effects by mechanisms involving up - regulation of the level and function of Bcl-2 protein . - pubmed
The pathophysiology of IPS secondary to high - dose busulfan - containing myeloablative regimens is not known , but cell - mediated immune reactions and release of cytokines may contribute to the lung injury . - pubmed
Our data showed that the expression of MRP1 - EGFP results in significantly decreased cellular accumulation of tetramethylrhodamine ethyl ester ( TMRE ) and daunorubicin , mildly decreased cellular accumulation of mitoxantrone , and decreased nuclear accumulation of doxorubicin . - pubmed
Additionally , MRP1 - EGFP expression protected cells from the microtubule depolymerization caused by vincristine and colchicine , but not by vinblastine . - pubmed
In fact , angiotensinogen is up - regulated particularly by oral administration of estrogen , whereas renin , angiotensin - converting enzyme ( ACE ) , and AT-1 receptor are down - regulated by the hormone . - pubmed
While under well - defined experimental conditions the net effect of estrogen appears to result in suppression of the renin - angiotensin system , the clinical situation may be more complex . - pubmed
Nevertheless , the undisputable , profound biochemical changes in the renin - angiotensin system related to the estrogen status allow speculation that such interaction explains some of the differences in the cardiovascular system of men and women . - pubmed
Naloxone blocked morphine - induced apoptosis suggesting involvement of an opiate receptor mechanism . - pubmed
Potent inhibition ( & gt;70 % ) of apoptosis by an inhibitor of caspase-3 as well as co - localization of active caspase-3 and DNA fragmentation in microglia or neurons treated with morphine indicated that caspase-3 is involved in the execution phase of morphine - induced apoptosis . - pubmed
In the presence of Na2HPO4 / NaH2PO4 , nanoparticles are agglutinated with phosphate ions as a glue to grow in size up to 60 - 100 nm , as revealed by DLS as well as microscopy ( TEM and AFM ) . - pubmed
The phosphate - induced agglutination processes can be followed by surface plasmon resonance ( SPR ) . - pubmed
Amphiphile 2n is readily immobilized on the hydrophobized sensor chip of SPR to give a closely packed monolayer with oligosaccharide moieties exposed to bulk water . - pubmed
The ATP - supported treadmilling of actin filaments , regulated by Actin Depolymerizing Factor ( ADF / cofilin ) , profilin and capping proteins provides the thermodynamic basis for sustained actin - based movement . - pubmed
Understanding the mechanism of actin - based movement requires elucidation of the biochemical properties and mode of action of Arp2 / 3 complex in filament branching , in particular the role of ATP binding and hydrolysis in Arp2 / 3 , and a physical analysis of the movement of functionalised particles . - pubmed
The Pearson coefficient was also found to correlate significantly and negatively with the indices of vagal modulation and positively with the indices of sympatho - vagal balance and rennin - angiotensin - aldosterone modulation in both normal controls and patients with AMI . - pubmed
CONCLUSIONS : Review indicated that ( 1 ) most published literature on fibre - based posts took the form of laboratory analyses ; ( 2 ) evidence for carbon - fibre posts far exceeds that for quartz - fibre posts ; ( 3 ) laboratory evidence was contradictory and could not be used to inform practice reliably ; ( 4 ) few clinical studies have been carried out though these have suggested fibre based posts may be clinically appropriate for restoration of the endodontically treated tooth ; and ( 5 ) controlled prospective clinical trials evaluating fibre - based posts should be undertaken to inform use for clinical practice . - pubmed
PMA , PA sup , and LPS increased MUC5AC gene expression and mucin protein production , effects that were prevented by pretreatment with AG1478 , a selective inhibitor of EGFR phosphorylation and by preincubation with an EGFR - neutralizing Ab or with a TGF - alpha - neutralizing Ab , implicating ligand ( TGF - alpha)-dependent EGFR phosphorylation in mucin production . - pubmed
Knockdown of TACE inhibited PMA- , PA sup- , and LPS - induced TGF - alpha shedding , EGFR phosphorylation , and mucin production . - pubmed
[ THE BEHAVIOR OF THE INSULIN ACTIVITY OF THE BLOOD AFTER ORAL GLUCOSE LOADING IN SUBJECTS WITH NORMAL METABOLISM AND IN DIABETICS ] . - pubmed
Therefore , possible involvement of lysosomal protease leakage caused by hydroxyl radical formation in lysosomes ( possibly resulting in mitochondrial membrane dysfunction ) is considered to play an important role in radiation - induced T cell apoptosis . - pubmed
INTERVENTIONS : First , a morning blood sample was obtained to measure baseline cortisol , corticotropin , interleukin-6 , and tumor necrosis factor alpha . - pubmed
RESULTS : PARP inhibition with the phenanthridinone - based PARP inhibitor PJ34 suppressed inflammatory cell migration . - pubmed
The unusual primary structure of this component was confirmed by mass spectrometric and amino acid sequence analyses of cytoplasmic protein extracts . - pubmed
Mechanism of cardiac depression after trauma - hemorrhage : increased cardiomyocyte IL-6 and effect of sex steroids on IL-6 regulation and cardiac function . - pubmed
Cardiomyocytes were isolated and divided into two parts : one was used for measurements of intracellular IL-6 levels using fluorescein - activated cell sorting , and the other was used to isolate RNA to determine IL-6 gene expression by quantitative real - time PCR . - pubmed
Our approach , which requires no experimental input apart from knowledge of bulk crystal structures , is demonstrated for palladium - copper alloys , which show nontrivial behavior due to the existence of face - centered cubic and body - centered cubic crystal structures and have the potential to resist sulfur poisoning . - pubmed
In contrast , responses of the diabetic ileum to the Ca2 + channel agonist Bay K8644 were greater than those of the controls , whilst the agonist failed to contract the colon . - pubmed
Similarly , the Ca2 + - ATPase inhibitors , thapsigargin and cyclopiazonic acid , produced contractions which were greater in diabetic ileal tissues . - pubmed
Furthermore , knockdown of p53 by pU6 - p53 siRNA vector suggests the involvement of MAP kinases in the process of 5 - aza - CdR - mediated activation of p53 pathway to inhibit cell proliferation and induce apoptosis . - pubmed
Finally , the comet or SCGE assay and methylation - sensitive restriction analysis demonstrated that 5 - aza - CdR induced p53 and p21Waf1 / Cip1 expression as a consequence of DNA damage and independent of DNA demethylation . - pubmed
Our findings suggest that 5 - aza - CdR induces anti - neoplastic activity primarily through the activation of p53 pathway in response to DNA damage and subsequently leads to inhibition of cell proliferation as well as induction of apoptosis . - pubmed
Therefore , our data indicate that p53 status in tumor cells may be critical for the clinical efficacy and toxicity of 5 - aza - CdR. - pubmed
Resistance to fluconazole and flucytosine was observed infrequently ( 3.7 % and 3.0 % ) ; 16.2 % of isolates were resistant to itraconazole ( MIC & gt ; or = 1 microg / ml ) . - pubmed
krusei ( 50 % S - DD , 41.7 % R , considered intrinsically fluconazole resistant ) . - pubmed
It is concluded that IRL 1620 , an ET ( B ) receptor agonist , does not alter paclitaxel pharmacokinetics and can selectively augment the delivery of paclitaxel to the tumour tissue . - pubmed
Breast cancer cell growth may be stimulated by 17beta - estradiol ( E2 ) or growth factors like epidermal growth factor ( EGF ) . - pubmed
Introduction of ERalpha and treatment with E2 decreased EGF - induced tyrosine phosphorylation of STAT5b , basal and EGF - induced STAT5 - mediated transcription , and EGF - stimulated DNA synthesis in these cells . - pubmed
Phenserine reduces the production of the amyloid precursor protein and beta - amyloid in vitro . - pubmed
Heat shock protein 70 as an indicator of early lung injury caused by exposure to arsenic . - pubmed
Hsp70 protein , mRNA and reactive oxygen species ( ROS ) generation were measured after exposing cells to arsenic as markers of injury . - pubmed
Hsp70 protein expression showed significant 7.9 - fold and 31.5 - fold increase using Western blotting and ELISA assay , respectively , at a 50 microM As ( III ) with a 12 h exposure and an 12 h recovery time . - pubmed
As ( III )-induced Hsp70 protein expression was inhibited significantly by catalase and NAC , indicating mediation of ROS in Hsp70 expression . - pubmed
These results suggest that cellular injury by arsenic is mediated through ROS generation resulting in the expression of Hsp70 . - pubmed
Freshly isolated UCB cells were cultured with IL-3 , IL-5 and GM - CSF in the presence or absence of a gamma - secretase inhibitor L-685,458 , and examined for the expression of major basic protein ( MBP ) . - pubmed
HbA(1c ) was 4.8 % and plasma aldosterone : renin ratios were elevated suggestive of increased aldosterone secretion . - pubmed
The adrenals were normal on CT scanning and blood pressure responded dramatically to the addition of spironolactone on day 212 ( 125/83 mmHg ) . - pubmed
CONCLUSIONS : The prevalence of primary hyperaldosteronism in the hypertensive population , based on elevation of plasma renin : aldosterone ratio , is approximately 6 % . - pubmed
Using the protein synthesis inhibitor cycloheximide we demonstrate that Hemolin up - regulation by 20E requires ongoing protein synthesis . - pubmed
CONCLUSION : The most common pattern of injectate spread seen on MRI with both approaches to PCB was spread within the body of the psoas muscle around the lumbar branches ( L2 - 4 ) , with cephalad spread to the lumbar nerve roots . - pubmed
We show that the S - phase - specific mec1 - 100 allele , like the sgs1Delta mutation , partially destabilizes DNA polymerases at stalled forks , yet combining the mec1 - 100 and sgs1Delta mutations leads to complete disassociation of the replisome , loss of RPA , irreversible termination of nucleotide incorporation , and compromised recovery from hydroxyurea ( HU ) arrest . - pubmed
Previous studies have shown that incubation of fibroblasts with leptomycin B resulted in the accumulation of galectin-3 in the nucleus , suggesting that the export of galectin-3 from the nucleus may be mediated by the CRM1 receptor . - pubmed
Rev ( 1.4 )- galectin-3(240 - 255 ) exhibited nuclear export activity that was sensitive to inhibition by leptomycin B. - pubmed
Site - directed mutagenesis of Leu247 and Ile249 in the galectin-3 nuclear export signal decreased nuclear export activity , consistent with the notion that these two positions correspond to the critical residues identified in the nuclear export signal of the cAMP - dependent protein kinase inhibitor . - pubmed
The nuclear export signal activity was also analyzed in the context of a full - length galectin-3 fusion protein ; galectin-3(1 - 263 ; L247A ) showed more nuclear localization than wild - type , implicating Leu247 as critical to the function of the nuclear export signal . - pubmed
These results indicate that residues 240 - 255 of the galectin-3 polypeptide contain a leucine - rich nuclear export signal that overlaps with the region ( residues 252 - 258 ) identified as important for nuclear localization . - pubmed
The majority of the adenosine 5 '- triphosphate ( ATP ) hydrolytic activity of purified vacuoles was associated with nonspecific acid phosphatase and not with a transport ATPase . - pubmed
As judged by acid phosphatase distribution and electron microscopy , the effective density of vacuoles in a sucrose gradient was low ( less than 1.1 grams per cubic centimeter ) , although an unequivocal estimate of the vacuole or tonoplast density was not possible from the experiments conducted . - pubmed
Furthermore , low O ( 2 ) prevented the rise in acid phosphatase activities and this suppression was not reversed by the inclusion of 100 microliters per liter ethylene in 2.5 % O ( 2 ) atmosphere . - pubmed
Low O ( 2 ) suppressed the appearance of all isoenzymes , and the addition of 500 microliters per liter ethylene to the low oxygen atmosphere did not reverse this effect . - pubmed
Similarly , the decline in pectin methylesterase that was observed in air - ripened fruits was prevented by 2.5 % O ( 2 ) alone and in combination with 500 microliters per liter ethylene . - pubmed
In this virus - host system , the extents of NF - kappaBeta - regulated gene expression and nuclear translocation were reduced in the presence of either PD 98059 or U0126 , two compounds capable of blocking ERK1 and ERK2 phosphorylation . - pubmed
First , ectopic M2L expression hampered ERK2 phosphorylation induced by exposure to phorbol myristate acetate . - pubmed
Further , altered cell growth resulting from serum withdrawal and the induction of caspase-3 activity by ultraviolet radiation were unchanged in the absence of PPARbeta / delta expression and/or the presence of GW0742 . - pubmed
While no changes in the expression of mRNAs encoding cell cycle control proteins were found in response to GW0742 , a significant decrease in the level of ERK phosphorylation was observed . - pubmed
KP-392 demonstrated increased tumor necrosis and decreased nuclear phospho - protein kinase / Akt but did not change the levels of phospho - extracellular signal - regulated kinase 1 / 2 . - pubmed
Why does the disorder of R - pn and rac - pn ligands in the quasi - one - dimensional bromo - bridged NiIII complexes , [ Ni ( pn ) 2Br ] Br2 ( pn = 1,2 - diaminopropane ) afford similar STM patterns ? - pubmed
The disordered patterns of R- and rac-1,2 - diaminopropane ( pn ) in quasi - one - dimensional bromo - bridged Ni ( III ) complexes , [ NiIII ( pn ) 2Br ] Br2 , have been investigated by single - crystal X - ray structure determination and scanning tunneling microscopy ( STM ) . - pubmed
We have concluded that tunnel current from the STM tip to metal ions are detected via methyl groups of pn ligands . - pubmed
This frameshift results in a truncated protein of 300 amino acids lacking the B30.2(SPRY ) domain , which we have named TRIM5theta . - pubmed
The activity of the enzyme , like that of most glycohydrolases , depends on a deprotonated carboxylate ( nucleophile ) and a protonated carboxylic acid for optimal activity . - pubmed
The resulting pH - profile of k(cat)/K(m ) for the beta - glucosidase - catalyzed hydrolysis of p - nitrophenyl glucoside is characterized by a width at half height that is strongly sensitive to the nature and concentration of the salt . - pubmed
There is a similar shift in the pK(a)s when the LiCl concentration is increased to 1.0 M. The results of these salt - induced pK(a ) shifts rule out a significant contribution of reverse protonation to the catalytic efficiency of the enzyme . - pubmed
Here we show that F - actin , detected by dye - coupled phalloidin , is a major molecular component of the karyosome capsule in Neuroptera . - pubmed
Vasodilation was measured by venous occlusion plethysmography after intra - arterial infusions of sodium nitroprusside and acetylcholine and insulin sensitivity by the intravenous glucose tolerance test using the minimal model technique . - pubmed
Lower feed intake , and therefore lower intake of the indispensable amino acids , may have resulted in lower pituitary GH and lower IGF - I mRNA levels in muscle tissues . - pubmed
In this study , we examined the role of catecholamines via alpha2 - adrenoreceptor in acute murine colitis . - pubmed
TH and DBH are induced in LPMCs of inflamed colon , the evidence of catecholamine synthesis during the process of colitis . - pubmed
RX821002 down - regulates the production of proinflammatory cytokines from LPMCs , while UK14304 leads to exacerbation of colitis . - pubmed
Together , our data show a critical role of catecholamines via alpha2 - adrenoreceptors in the progress of acute colitis , and suggest that use of the alpha2 - adrenoceptor antagonist represents a novel therapeutic approach for the management of colitis . - pubmed
Repetitive alcohol ingestion and its resulting dependence is associated with false euphoria triggered by an inhibition of glutamate receptors and other brain neurotransmitters , namely dopamine and serotonine . - pubmed
The QSRR model and DFT / MP2 studies suggest that retention times of alkylnaphthyl ketones on silver - containing stationary phases are determined primarily by the dipole moment , length of the alkyl substituent and concentration of modifier in the mobile phase . - pubmed
METHODS : Lipids were extracted from tears and worn contact lenses using 2:1 chloroform : methanol and the extract washed with aqueous ammonium acetate , before analysis by electrospray ionization tandem mass spectrometry ( ESI - MS / MS ) . - pubmed
Blockade of [ 18F ] MK-1312 uptake with mGluR1 allosteric antagonist MK-5435 dose - dependently reduced tracer uptake in all regions of gray matter to a similarly low level of tracer uptake . - pubmed
Taken together , these results are promising for clinical PET studies with [ 18F ] MK-1312 to determine mGluR1 occupancy of MK-5435 . - pubmed
tRNA : m ( 5 ) C methyltransferase Trm4 generates the modified nucleotide 5 - methylcytidine in archaeal and eukaryotic tRNA molecules , using S - adenosyl - l - methionine ( AdoMet ) as methyl donor . - pubmed
jannaschii Trm4 and its complex with sinefungin at 1.27 A and 2.3 A resolutions , respectively . - pubmed
Adjacent to the sinefungin - bound pocket , highly conserved residues form a large , positively charged surface , which seems to be suitable for tRNA binding . - pubmed
The short - term success of imiquimod 5 % cream in randomized controlled trials comparing different treatment regimes and dosing as a treatment for small superficial basal cell carcinoma ( BCC ) not on the face or neck is in the range of 82 % for 5 times per week application . - pubmed
Paracingulin is a 160 - kDa protein localized in the cytoplasmic region of epithelial tight and adherens junctions , where it regulates RhoA and Rac1 activities by interacting with guanine nucleotide exchange factors . - pubmed
The proportions of NK cells ( CD3 - CD56 + ) and hematopoetic stem cells ( CD34+lin- ) were increased after natalizumab treatment . - pubmed
Drug regimens were categorized into 4 mutually exclusive groups : ( 1 ) tumor necrosis factor ( TNF ) inhibitors with or without other DMARDs ; ( 2 ) methotrexate without TNF inhibitors or hydroxychloroquine ; ( 3 ) hydroxychloroquine without TNF inhibitors or methotrexate ; or ( 4 ) other nonbiologic DMARDs without TNF inhibitors , methotrexate , or hydroxychloroquine ( reference exposure ) . - pubmed
New diabetes cases and respective incidence rates per 1000 person - years were : other nonbiologic DMARDs ( 55 cases among 3993 treatment episodes ; rate , 50.2 ; 95 % confidence interval [ CI ] , 47.3 - 53.2 ) ; TNF inhibitors ( 80 cases among 4623 treatment episodes ; rate , 19.7 ; 95 % CI , 19.1 - 20.3 ) ; methotrexate ( 82 cases among 8195 treatment episodes ; rate , 23.8 ; 95 % CI , 23.0 - 24.6 ) ; and hydroxychloroquine ( 50 cases among 5682 treatment episodes ; rate , 22.2 ; 95 % CI , 21.3 - 23.1 ) . - pubmed
The multivariate adjusted hazard ratios for DM were 0.62 ( 95 % CI , 0.42 - 0.91 ) for TNF inhibitors , 0.77 ( 95 % CI , 0.53 - 1.13 ) for methotrexate , and 0.54 ( 95 % CI , 0.36 - 0.80 ) for hydroxychloroquine compared with other nonbiologic DMARDS . - pubmed
CONCLUSION : Among patients with RA or psoriasis , the adjusted risk of DM was lower for individuals starting a TNF inhibitor or hydroxychloroquine compared with initiation of other nonbiologic DMARDs . - pubmed
Hippocampi were processed for spectrophotometric measurements of the products of oxidative lipid damage , thiobarbituric acid - reactive substances ( TBARS ) levels , as well as the activities of antioxidant enzymes , superoxide dismutase ( SOD ) , and glutathione peroxidase ( GSH - Px ) . - pubmed
Enoxaparin significantly decreased the hippocampal TBARS and oxidized protein levels , COX-2 overexpression and reactive gliosis , but it did not influence the SOD and GSH - Px activities , pro - IL-1beta and active caspase-3 overexpressions as well as neurodegeneration following TBI . - pubmed
METHODS : I have reviewed the literature bases of Google Scholar and the National Library of Medicine using the key words : history , opium , spinal analgesia , spinal morphine , intrathecal ( IT ) , opioid receptors , endogenous opioids , IT delivery of opioids , and IT side - effects / complications . - pubmed
The depletion of endoplasmic reticulum ( ER ) Ca2 + is sensed by stromal interaction molecule 1 ( STIM1 ) . - pubmed
On store depletion , STIM1 aggregates and moves to areas where the ER comes close to the plasma membrane ( PM ; within 25 nm ) to interact with Orai1 channels and activate Ca2 + entry . - pubmed
Greater levels of GH are induced in T cell - enriched populations compared to B cell - enriched populations after treatment with CoCl ( 2 ) or TMA . - pubmed
Using cultured basal forebrain ( BF ) neurons , this study demonstrates selective internalization of amyloid beta ( Abeta ) 1 - 42 in conjunction with p75 ( NTR ) ( labelled with IgG192 - Cy3 ) by cholinergic cells . - pubmed
Active under resting conditions , this process was enhanced by high K (+ ) stimulation and was insensitive to inhibitors of regulated synaptic activity - tetrodotoxin or botulinum neurotoxins ( BoNT type / A and / B ) . - pubmed
Blockade of sarco - endoplasmic reticulum ( SERCA ) Ca ( 2 + ) ATPase with thapsigargin and CPA or chelation of Ca ( 2 + ) with EGTA - AM strongly suppressed the endocytosis of p75 ( NTR ) , implicating the role of ER released Ca ( 2 + ) . - pubmed
The uptake of IgG192 - Cy3 was also reduced by T - type Ca ( 2 + ) channel blocker mibefradil but not Cd ( 2 + ) , an indiscriminate blocker of high voltage - activated Ca ( 2 + ) currents . - pubmed
A strong co - localization of IgG192 - Cy3 with late endosome ( Rab7 ) or lysosome ( Lamp1 ) qualifier proteins suggest these compartments as the primary destination for internalized IgG192 and Abeta . - pubmed
Females then experienced 10 alternating trials of distributed CLS or no CLS paired with one side of a non - biased CPP box under the same hormone - priming regimen . - pubmed
OBJECTIVES : Casein phosphopeptide - amorphous calcium phosphate ( CPP - ACP ) has the potential to induce the biomimetic mineralisation of dentine collagen fibrils . - pubmed
This study aimed to demonstrate in vitro the ability of CPP - ACP to form apatite crystals on phosphorylated dentine collagen fibrils . - pubmed
The slices were etched with phosphoric acid to expose the collagen fibres . - pubmed
Sodium trimetaphosphate was then used to phosphorylate the exposed collagen fibres . - pubmed
CPP - ACP paste was topically applied to the surface of the phosphorylated slices , which were then immersed in a metastable calcium phosphate remineralising solution and incubated at 37 C for 10 days . - pubmed
CONCLUSIONS : CPP - ACP can induce the biomimetic mineralisation of dentine through apatite formation along and between the phosphorylated dentine collagen fibres . - pubmed
Plasma 25 -( OH ) D , RBP-4 and NGAL concentrations were measured with specific assays . - pubmed
In the entire BMI range , Spearman correlations revealed strong positive associations between 25 -( OH ) D and RBP-4 ( r=0.349 , p=0.002 ) and between 25 -( OH ) D and NGAL ( r=0.338 , p=0.003 ) . - pubmed
The probes were aggregated nonspecifically to form large clusters , which showed a strong resonance light scattering ( RLS ) peak at 520 nm , under preparation conditions ( pH 7.6 Na2HPO4 - NaH2PO4 buffer and ultrasonication ) . - pubmed
Upon addition of BPA , the probe reacted specifically to form dispersed BPA - Apt - AuNP conjugates that exhibited strong catalysis of the two particle reactions of glucose - Cu ( II ) and hydrazine hydrochloride - Cu ( II ) with a strong RLS peak at 360 nm and 510 nm respectively . - pubmed
When the BPA concentration increased , the RLS intensity at 360 nm and 510 nm increased respectively . - pubmed
Accordingly , two new and highly - sensitive RLS methods were established for the detection of BPA , using the Apt - AuNP catalytic amplification . - pubmed
AIMS : To examine the associations between antihypertensive treatment with angiotensin - converting enzyme inhibitors ( ACEis ) or angiotensin receptor blockers ( ARBs ) , beta - blockers , diuretics , or calcium - antagonists , and risk of atrial fibrillation . - pubmed
The review is presented on the infusion of sterile water ( hypotonic fluid ) to lower serum sodium level in those circumstances when enteral supplementation of water is not possible , such as in postoperative patients or when other isotonic fluids ( such as 5 % dextrose in water infusion ) are less than ideal - for example , hyperglycaemic patients on an insulin infusion . - pubmed
The significant technical improvement on state - of - the - art is the demonstration of consecutive , spatio - temporally profiling of glycans on a single cell by sequential elution of the previous lectin probe using their corresponding free sugar . - pubmed
METHODS : We present a 4 - year study with 262 unselected consecutive high - risk episodes , prospectively managed with posaconazole primary prophylaxis and a uniform diagnostic algorithm , including biweekly serum GM quantification for early detection of invasive aspergillosis . - pubmed
The synthesis of the metal - chelator phytochelatin ( PCs ) and relative levels of transcripts encoding the enzymes gamma - glutamylcysteine synthetase ( gamma - GCS ) , glutathione synthase ( GS ) and phytochelatin synthase ( PCS ) that participate in the PC biosynthetic pathway were measured , along with the effects on growth , and adsorption and uptake of Cu . - pubmed
In Es524 there was an increase in the transcripts of gamma - GCS , GS and PCS , particularly under high Cu exposure , whereas in LIA4 transcripts of gamma - GCS1 increased only slightly , gamma - GCS2 and GS decreased and PCS did not change . - pubmed
ABT-639 is a peripherally acting highly selective T - type Ca ( v ) 3.2 calcium channel blocker that has demonstrated analgesic efficacy in preclinical models and may have the potential to reduce spontaneous fiber activity . - pubmed
Sudan III fat staining , viability and proliferation assays , electric cell - substrate impedance sensing , real - time PCR for gene expression of cyclooxygenase-2 and 15 - lipoxygenase and ELISAs for resolvin D1 ( RvD1 ) , IFNgamma , TNFalpha and IL-6 were applied . - pubmed
IL-6 and IFNgamma were downregulated in HMGECs treated for 72 h with DHA and EPA . - pubmed
In general , TNFalpha , IFNgamma and IL-6 levels were decreased after 72 h compared to 24 h in serum containing medium with or without DHA or EPA . - pubmed
Cyclooxygenase-2 gene expression decreased compared to controls during DHA stimulation after 72 h. Treatment with DHA and ASA revealed a decreased 15 - lipoxygenase gene expression which was reduced after three days of DHA incubation . - pubmed
DHA and EPA supplementation affected HMGECs in vitro and supported anti - inflammatory effects by influencing cytokine levels , decreasing COX-2 expression and increasing the production of RvD1 . - pubmed
The results showed significant differences between the three strains which may be explained , at least in part , by variations in the distribution of carbon source between two glycolytic pathways during the bacterial growth : the phosphoketolase or heterolactic pathway ( PKP ) and the Embden - Meyerhof pathway ( EMP ) . - pubmed
Recently , we have provided evidence for therapeutic efficacy of anti - IGF1R - targeted , surface modified doxorubicin loaded liposomes . - pubmed
In the current study , post - modification of sterically stabilized liposomal Dox was thoroughly investigated including the influence of different modification techniques ( PIT , SPIT , SPIT60 ) , lipid composition ( SPC / Chol , HSPC / Chol ) , and buffers ( HBS , SH ) . - pubmed
Prohibitin promotes apoptosis of promyelocytic leukemia induced by arsenic sulfide . - pubmed
Prohibitin ( PHB ) was selected for its diverse role and its increased expression in the cells treated with As4S4 . - pubmed
PML - RARalpha fusion protein was found to be reduced with PHB - overexpression , and following As4S4 treatment , a greater reduction of promyelocytic leukemia - retinoic acid receptor - alpha ( PML - RARalpha ) fusion protein was seen in PHB - overexpression than that in parental cells . - pubmed
Niemann - Pick disease type A ( NP - A ) and type B ( NP - B ) are lysosomal storage diseases ( LSDs ) caused by sphingomyelin accumulation in lysosomes relying on reduced or absent acid sphingomyelinase . - pubmed
AIM OF THE STUDY : Extended spectrum beta lactamase ( ESBL ) producing bacteria are resistant to most beta - lactam antibiotics including third - generation cephalosporins , quinolones and aminoglycosides . - pubmed
Relationship between plasma and salivary melatonin and cortisol investigated by LC - MS / MS . - pubmed
METHODS : Here we describe the validation and application of a high - throughput liquid chromatography in combination with mass spectrometry ( LC - MS / MS ) method for the combined analysis of melatonin and cortisol in plasma and saliva . - pubmed
In this study , five DBS leads were modified using a cost - effective and materials - efficient process for applying an ultra - low impedance platinum - iridium alloy coating . - pubmed
The platinum - iridium alloy significantly improved the electrical properties of the DBS electrodes and was robust to insertion into brain and to 12 weeks of chronic pulsing . - pubmed
All 18 imipenem - resistant ceftazidime - susceptible isolates showed decreased mRNA expression of oprD , and overexpression of mexB was observed in 13 isolates . - pubmed
Antibiotics like bacitracin methylene disalicylate ( BMD ) and avilamycin ( AVL ) are commonly used antibiotic growth promoters ( AGP ) in poultry feed to control necrotic enteritis in birds . - pubmed
As the number of paced beats increased from 1 to 5 , SR Ca content ( assessed with caffeine pulses ) increased , the number of Ca wave initiation sites increased , integrated Ca transients and DADs became larger and shorter in duration , and the latency period to the onset of Ca waves shortened with reduced variance . - pubmed
In silico analysis using a computer model of ventricular tissue incorporating these experimental measurements revealed that whereas all of these factors promoted larger DADs with higher probability of generating triggered activity , the latency period variance and SR Ca load had the greatest influences . - pubmed
Therefore , incorporating quantitative experimental data into tissue level simulations reveals that increased intracellular Ca promotes DAD - mediated triggered activity in tissue predominantly by increasing both the synchrony ( decreasing latency variance ) of Ca waves in nearby myocytes and SR Ca load , whereas the number of Ca wave initiation sites per myocyte is less important . - pubmed
Epidermal growth factor signals attenuate phenotypic and functional development of neocortical GABA neurons . - pubmed
Phenotypic development of neocortical GABA neurons is highly plastic and promoted by various neurotrophic factors such as neuregulin-1 . - pubmed
A subpopulation of GABA neurons expresses not only neuregulin receptor ( ErbB4 ) but also epidermal growth factor ( EGF ) receptor ( ErbB1 ) during development , but the neurobiological action of EGF on this cell population is less understood than that of neuregulin-1 . - pubmed
Here , we examined the effects of exogenous EGF on immature GABA neurons both in culture and in vivo and also explored physiological consequences in adults . - pubmed
A Fe3O4@Au - basedpseudo - homogeneous electrochemical immunosensor for AFP measurement using AFP antibody - GNPs - HRP as detection probe . - pubmed
In this study , a Fe3O4@Au - based pseudo - homogeneous electrochemical immunosensor was prepared for detection of alpha fetoprotein ( AFP ) , a well - known hepatocellular carcinoma biomarker . - pubmed
The primary antibody ( Ab1 ) was immobilized on Fe3O4@Au NPs as the capture probe . - pubmed
In the presence of AFP , a sandwich immunocomplex was formed via specific recognition of antigen - antibody in a Fe3O4@Au - basedpseudo - homogeneousreaction system . - pubmed
After the immunocomplex was captured to the surface of magnetic glassy carbon electrode ( MGCE ) , the labeling HRP catalyzed the decomposition of H2O2 , resulting in a substantial current for the quantitative detection of AFP . - pubmed
The amperometric ( i - t ) method was employed to record the response signal of the immunosensor based on the catalysis of the immobilized HRP toward the reduction of H2O2 with hydroquinone ( HQ ) as the redox mediator . - pubmed
On L1 and L2 larval stages after 24 h of exposure to extracts , the mortality rates of 100 % , 54.76 % and 96.77 % against 98 % , 51.44 % and 100 % were obtained for MCM As , MCM Nl and MCM As - Nl respectively at the highest concentration . - pubmed
Here , we review some of the molecular mechanisms through which insulin modulates hepatic gluconeogenesis , thus controlling glucose production by the liver to ultimately maintain normoglycemia . - pubmed
Codelivery of doxorubicin and MDR1 - siRNA by mesoporous silica nanoparticles - polymerpolyethylenimine to improve oral squamous carcinoma treatment . - pubmed
In this study , we tried to fabricate a novel nanoparticle that could carry both MDR1 - siRNA to block MDR1 expression and doxorubicin ( DOX ) , a chemotherapy drug , into cancer cells in order to directly kill the cells with little or no effect of multidrug resistance . - pubmed
Results showed that mesoporous silica nanoparticles ( MSNP ) can be modified by cationic polymerpolyethylenimine ( PEI ) to obtain positive charges on the surface , which could enable the MSNP to carry MDR1 - siRNA and DOX . - pubmed
Importantly , MSNP - PEI - DOX / MDR1 - siRNA dramatically reduced the tumor size ( 81.64 % decrease after 28 days posttreatment ) and slowed down tumor growth rate compared to the control group in vivo ( P<0.05 ) . - pubmed
In the aggregate , newly synthesized MSNP - PEI - DOX / MDR1 - siRNA improves cancer chemotherapy effect in terms of treating multidrug - resistant cancer compared to DOX only , clearly demonstrating that MSNP - PEI - DOX / MDR1 - siRNA has potential therapeutic application for multidrug - resistant cancer in the future . - pubmed
Kyoto Encyclopedia of Gene and Genome analysis showed that the target genes are involved in metabolic pathways , mitogen - activated protein kinase signaling pathway , calcium signaling pathway , and nuclear factor - kappaB signaling pathway . - pubmed
Conclusion : ESP block is easy to perform and can serve as a promising alternative to TEA in optimal perioperative pain management in cardiac surgery . - pubmed
The resulting hypercalcemia is a result of 1 - alpha hydroxylase overexpression in granulomas with increased 1,25 - dihydroxy vitamin D levels . - pubmed
1,25 - dihydroxy vitamin D levels were markedly elevated with low normal 25 - hydroxy vitamin D levels , angiotensin - converting enzyme levels were also high , and chest computed tomography ( CT ) imaging was negative for any lymphadenopathy ( including perihilar lymphadenopathy ) . - pubmed
Consequently , the CHOP downstream targets such as phospho - JNK and death receptor 5 were also elevated , along with the cleavage of caspase-8 , - 3 , and the ER stress - related caspase-4 . - pubmed
We have now investigated whether depletion of the recently identified third galanin receptor , GAL3 - R , and that of GAL2 - R , alters the threshold to the systemically applied gamma - aminobutyric acid ( GABA ) antagonist pentylenetetrazole ( PTZ ) or to intrahippocampally administered kainic acid ( KA ) . - pubmed
Amplicon sequencing of the hypervariable V3 - V4 regions of the 16S rRNA gene revealed that microbial communities remained stable across CO2 seep sites ( pH = 7.44 - 7.85 ) and adjacent control sites ( ambient pH = 8.0 - 8.1 ) . - pubmed
Parameters of glucose and lipid metabolisms were measured for the pups on post - natal day 21 , 26 and 60 . - pubmed
At weaning , insulin secretion upon glucose stimulation was unchanged , but the removal of circulating glucose was slower for pups born from the lowest impregnated dams ( Cd1 ) . - pubmed
Furthermore , pups from dams accumulating the highest levels of Cd ( Cd2 ) exhibited a compensatory increased insulin pancreatic secretion , together with increased circulating non - esterified fatty acids , indicating the establishment of insulin resistance , 2 months after birth . - pubmed
In this paper , a facile one - pot freeze - drying self - assembly approach combined with in - situ thermal decomposition - reduction method is proposed to construct 3D porous aerogels with MoS2 active materials anchored on graphene backbone , making a highly interconnected network with desirable structural stability . - pubmed
We compared DON with a state - of - art disease phenotype network , disease manifestation network ( DMN ) , that we have recently constructed , and demonstrated that DON contains complementary knowledge for disease genetics understanding . - pubmed
OBJECTIVES : Primary objectivesTo determine the effectiveness and safety of ibuprofen compared to placebo / no intervention , or other cyclo - oxygenase inhibitor drugs in the prevention of PDA in preterm infants . - pubmed
SELECTION CRITERIA : Randomised and quasi - randomised controlled trials comparing ibuprofen with placebo / no intervention or other cyclo - oxygenase inhibitor drugs to prevent PDA in preterm or low birth weight infants . - pubmed
Ibuprofen ( IV or oral ) probably decreases the risk of PDA on day 3 or 4 ( typical RR 0.39 , 95 % CI 0.31 to 0.48 ; typical RD -0.26 , 95 % CI -0.31 to -0.21 ; NNTB 4 , 95 % CI 3 to 5 ; 9 trials ; N = 1029 ) ( moderate - quality evidence ) . - pubmed
In addition , ibuprofen probably decreases the need for rescue treatment with cyclo - oxygenase inhibitors ( typical RR 0.17 , 95 % CI 0.11 to 0.26 ; typical RD -0.27 , 95 % CI -0.32 to -0.22 ; NNTB 4 ; 95 % CI 3 to 5),and the need for surgical ductal ligation ( typical RR 0.46 , 95 % CI 0.22 to 0.96 ; typical RD -0.03 , 95 % CI -0.05 to -0.00 ; NNTB 33 , 95 % CI 20 to infinity ; 7 trials ; N = 925 ) ( moderate - quality evidence ) . - pubmed
AUTHORS ' CONCLUSIONS : This review shows that prophylactic use of ibuprofen , compared to placebo or no intervention , probably decreases the incidence of patent ductus arteriosus , the need for rescue treatment with cyclo - oxygenase inhibitors , and for surgical ductal closure . - pubmed
Adverse effects associated with ibuprofen ( IV or oral ) included increased risks for oliguria , increase in serum creatinine levels , and increased risk of gastrointestinal haemorrhage . - pubmed
Transient tyrosine and tryptophan radicals play key roles in the electron transfer ( ET ) reactions of photosystem ( PS ) II , ribonucleotide reductase ( RNR ) , photolyase and many other proteins . - pubmed
Cytochrome c peroxidase ( CcP ) employs a Trp191 + radical to oxidize reduced cytochrome c ( Cc ) . - pubmed
Here we probe the redox properties of CcP Y191 by non - natural amino acid substitution , altering the ET driving force , and manipulating the protic environment of Y191 . - pubmed
Taken together , studies of inhibition of hemagglutinating activity , fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain ( CRD ) , suggesting that the results obtained in this study may be directly related to the interaction of DVL - gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum . - pubmed
Trends in HbA1c and LDL Cholesterol in Patients With Type 2 Diabetes Receiving First - Time Treatment in Northern Denmark , 2000 - 2017 : Population - Based Sequential Cross - Sectional Analysis . - pubmed
We first use a B12 - responsive transcription factor to enable B12 - dependent output in a cell - free reaction , but the resulting sensor responds to B12 far above clinically relevant concentrations . - pubmed
Blood samples were probed for LPS - mediated cytokine production by incubating them with LPS inhibitor polymyxin B ( PMB ) for 24 h at 37 C besides the Limulus amebocyte lysate ( LAL ) assay . - pubmed
PMB suppressed IL-1beta ( P = 0.035 ) and IL-6 ( P = 0.0487 ) production in the 3 h PPB of the PS after 24 h incubation at 37 C compared to the vehicle control , suggesting the presence of LPS . - pubmed
Sulfated vizantin disrupted the localization balance between cell - associated glucosyltransferase and cell - free glucosyltransferase , resulting in inhibited biofilm maturation . - pubmed
The KIRs and HLA ligands were identified in 235 healthy controls ( HC ) and 253 dengue patients ( DEN ) using polymerase chain reaction with sequence specific primer ( PCR - SSP ) ; moreover , DEN was classified to 100 dengue fever ( DF ) and 153 dengue haemorrhagic fever ( DHF ) . - pubmed
Other quality indexes of soy sauce , including RS , TA , and AN , were not greatly affected . - pubmed
The results illustrate the accuracy of the Figaro TGS 2600 sensor when methane is quantified from raw signals using the techniques described . - pubmed
A possibility that the anticataleptic action of chlorcyclizine , cyproheptadine , diphenhydramine , mepyrymine and danitracen depends on the blockade of the histamine receptors in the brain is discussed . - pubmed
The effect of delagil ( chloroquine ) and of hydrocortisone taken in different concentration on the lymphocytes blast - transformation reaction in donors , stimulated with streptococcal nucleoprotein , was enquired into . - pubmed
The excretion profile of the combined hydrolysis products was compared with the excretion profile of a different group of metabolites of sulfur mustard derived from the glutathione / beta - lyase pathway . - pubmed
The excretion of beta - lyase products showed a much sharper decline than was observed for the hydrolysis products of sulfur mustard . - pubmed
Mutant Era - V17 purified from the strain was cold - sensitive for GTP - hydrolytic activity , suggesting that the GTPase activity of Era is required for cell growth since the P17V mutation resulted in both cold - sensitive growth of cells and cold - labile GTPase activity of the purified protein . - pubmed
Interstitial cells were prepared by collagenase digestion and used to measure 125I - labelled human chorionic gonadotrophin ( hCG ) binding capacity and androgen production in the presence or absence of hCG or dibutyryl cyclic AMP ( dbcAMP ) . - pubmed
At day 2 after EDS treatment , 125I - labelled hCG binding capacity was reduced to 10 % of control values , while the production of testosterone and 5 alpha - androstane-3 alpha , 17 beta - diol ( adiol ) were non - detectable . - pubmed
Between days 24 and 40 post - treatment , Leydig cell regeneration occurred , as indicated by a rise in 125I - labelled hCG binding capacity , increased androgen production and the presence of histologically identifiable Leydig cells . - pubmed
Western blots with anti - tubulin antibody showed that the albendazole resistant strain had an altered tubulin . - pubmed
It is postulated that bldA specifies a tRNA that would recognize the codon UUA ( for leucine ) . - pubmed
Previous studies have demonstrated that near - complete immunosuppression is obtained with FK at 10(-8 ) M and CsA at 10(-6 ) M. When cultured up to 72 h throughout the concentration range , neither FK nor CsA altered HFP cellular architecture , and indirect immunoperoxidase staining for insulin demonstrated no change in quantity or distribution of insulin - producing beta - cells compared with control tissue . - pubmed
Drug - cultured HFP also retained the ability to release insulin in response to a glucose / theophylline challenge . - pubmed
In calcium containing buffer , endothelin-1 induced a concentration - dependent 45Ca2 + efflux response over the range of 10 pM to 100 nM with an EC50 of approximately 60 pM. - pubmed
Maximum endothelin - stimulated 45Ca2 + efflux was not affected by the absence of extracellular calcium or the presence of 1 microM verapamil . - pubmed
Endothelin-1 did not induce transplasmalemmal 45Ca2 + uptake at times up to 30 min . - pubmed
Staining was minimal or absent for protein C , protein S , plasminogen activator inhibitors 1 - 3 , factor VII , factor X , and fibrin ( the antigenic site on the amino - terminal portion of B beta chain that is exposed following thrombin cleavage of fibrinopeptide B was not detected ) . - pubmed
The precursor contains three functional domains , the amino - terminal leader which is assumed to initiate the inner membrane transport of the precursor , the protease , and a carboxyl - terminal ' helper ' domain apparently required for extracellular secretion ( excretion ) . - pubmed
Unaccounted for by an increase in viable ovarian cell numbers and independent of the hCG dose ( 0.1 - 10 ng / ml ) used , the Sm - C / IGF - I effect proved time and dose dependent , with a projected minimal effective dose of 3 ng / ml and a minimal time requirement of 72 h. [ 125I ] Iodo - Sm - C / IGF - I binding to untreated highly enriched thecal - interstitial cells proved saturable , with a single class ( Hill coefficient = 0.98 + /- 0.01 ) of high affinity ( Kd = 3.0 nM ) , low capacity ( maximum binding = 10,840 + /- 2,108 sites / cell ) binding sites . - pubmed
Taken together , these findings suggest that 1 ) the thecal - interstitial cell , like the granulosa cell , may be a site of Sm - C / IGF - I reception and action , and 2 ) the ability of high dose insulin to stimulate ovarian androgen biosynthesis may be due to its capacity to act as a Sm - C / IGF - I surrogate , its high dose requirements reflecting cross - interaction with the type I receptor. - pubmed
PAMP-1 has generally been regarded as an ' oestrogen - inducible ' protein regulated by the oestrogen / androgen balance . - pubmed
Lipolysis was measured by glycerol release after either incubation or perifusion with the following concentrations : epinephrine ( EPI ) , 0.01 - 0.1 microM ; theophylline , 0.01 - 1.0 mg / ml ; adenosine deaminase ( ADA ) , and bovine GH ( bGH ) , 0.01 - 1.0 microgram / ml . - pubmed
In a dose - response study comparing glycerol release from control and STZ - DM IFC , IFC were preincubated with 1.0 microgram / ml bGH and then incubated with varying concentrations of EPI or bGH . - pubmed
In STZ - DM , we noted increased lipolytic sensitivity to low concentrations of EPI or bGH . - pubmed
Furthermore , in perifusion , STZ - DM IFC did not require obligatory preincubation with bGH for optimal glycerol release . - pubmed
In both systems an enhanced lipolytic response to theophylline was seen in the presence of bGH in control and STZ - DM . - pubmed
Preincubation with GH increases the lipolytic response of IFC from STZ - DM to all lipolytic agents compared to control responses . - pubmed
One of these , the cytochrome - based electron transport system , produced anaerobic conditions by the reduction of oxygen dissolved in the medium . - pubmed
The reactions of the sulfhydryl groups on the elongation factors Tu and Ts . - pubmed
The observed changes in enzymic activities are consistent with the possibility that RFP induces a special form of cytochrome P-450 , responsible for the metabolism of the antibiotic ( demethylation and reduction of rifampicin quinone ) . - pubmed
Cytochrome P-450 concentration was not greatly affected , with only a 40 % increase by clofibrate ( dose 200 mg / kg / day ) and by F1379 at the lower dose . - pubmed
It is of interest that all the hypolipidaemic agents tested enhanced the activity of epoxide hydrolase with 4 , 5 benzo ( a ) pyrene oxide as the substrate . - pubmed
We also measured UDP - glucuronosyltransferase activities using two substrates belonging to group I ( 4 - nitrophenol ) and group II ( 4 - hydroxybiphenyl ) . - pubmed
This study showed that hypolipidaemic drugs which are chemically related to clofibrate could greatly modify the activity of drug metabolizing enzymes and therefore alter the transformation of drugs administered concomitantly. - pubmed
Membrane modification ( administration of cholesterol , trypsin ) influences the PC exchange ; changes of microviscosity brings about the exchange , but the interrelation is of very complicated character . - pubmed
D. Kallikrein - kinin system and prostaglandins system ] . - pubmed
Commercially available concanavalin A binds Ca2 + with high apparent affinity . - pubmed
The concanavalin A stimulated uptake of 45Ca2 + occurred within minutes , and required concentrations of concanavalin A which promoted [ 3H ] thymidine uptake into these cells . - pubmed
Although the concanavalin A - promoted Ca2 + uptake occurs at lectin concentrations that cause lymphocyte proliferation as measured by 3H - thymidine incorporation , the role of Ca2 + in this event remains unclear . - pubmed
CONCLUSIONS : The phase II dose of doxorubicin in this combination therapy has been established at 165 mg / m2 , with dose - limiting toxicity defined as mucositis . - pubmed
The two alleles were found to differ by a single TA to GC transversion , altering one amino acid in the deduced amino acid sequence . - pubmed
We tested the hypothesis that muscle cell types at rest have intrinsically different contents of PCr , ATP and Pi . - pubmed
The retinol level below 30 MK2 / dl ( lower reference level ) was revealed in 6 % of the adults , 19 % of the children and 6 % of the pregnant women . - pubmed
The level of carotene below 80 MK2 / dl took place in 39 % of the adults , 28 % of the children and 18 % of the pregnant women . - pubmed
Effects of HMG - CoA reductase inhibition on erythrocyte membrane cholesterol and acyl chain composition . - pubmed
These results suggest that the chemical potential of cholesterol in serum is not markedly altered by HMG - CoA reductase inhibition . - pubmed
Variation in sulfation pattern on aggrecan chondroitin sulfate side chains is also observed in the hypertrophic region with an increasing proportion of unsulfated residues present , which may play a role in the initiation of mineralization . - pubmed
The existence of cryptic genes , responsible for growth on alternative substrates , was previously deduced from the analysis of other mutants obtained as a response to the restrictive condition on media containing non - assimilating carbon sources . - pubmed
Regulation of the adenohypophyseal thyrotropin - releasing hormone - degrading ectoenzyme by estradiol . - pubmed
We describe here gender - related differences and the effect of estradiol ( E2 ) on the expression of this enzyme in the anterior pituitary . - pubmed
Because the expression of the adenohypophyseal TRH - degrading ectoenzyme is tightly regulated by both T3 and E2 with adequate dynamics , we conclude that this peptidase serves integrative functions for the control of TRH - stimulated hormone secretion . - pubmed
In contrast to PEC-60 and somatostatin , it has no established inhibitory effect on insulin secretion . - pubmed
Like gastrointestinal trefoil peptides , dopuin has three disulphide bridges , Ala - Pro segments , and many charged residues , but they are differently distributed and dopuin belongs to a separate , apparently novel family . - pubmed
However , the tension - time integrals indicated an equivalent capacity of sequestrable Ca2 + in SR from both tissues . - pubmed
In atrial , as in ventricular myocardium , the SR Ca2 + uptake was more efficiently supported by ATP produced by the SR - bound MM form of creatine kinase ( CK ; MM - CK ) than by externally added ATP , suggesting a tight functional coupling between the SR Ca2 + adenosinetriphosphatase ( ATPase ) and MM - CK . - pubmed
CaMKIIPase showed apparent molecular weights of 54,000 and 65,000 , on SDS - polyacrylamide gel electrophoresis and gel - filtration analysis , respectively . - pubmed
The stimulated Ca ( 2 +)-independent activity of autophosphorylated CaMKII was reversed by the action of CaMKIIPase . - pubmed
Insulin secretion in response to an oral glucose tolerance test ( OGTT ) and a GH stimulation test by L - dopa , growth hormone - binding protein ( GHBP ) and IGF - I were measured . - pubmed
The insulin and free fatty acid ( FFA ) areas under curves ( AUC ) during the OGTT , and the IGF - I level , were also positively correlated with GHBP levels ( r = 0.474 , P<0.005 ; r = 0.572 , P<0.005 ; r = 0.453 . - pubmed
GH - AUC to the L - dopa stimulation test was negatively correlated with GHBP levels ( r = -0.432 . - pubmed
This activation is dependent on the presence of high molecular weight kininogen and an optimal zinc ( Zn2 + ) concentration . - pubmed
Further kallikrein formed on endothelial cell membranes is capable of cleaving its receptor and native substrate , high molecular weight kininogen , liberating bradykinin and terminating activation . - pubmed
h(-1)provided an anti - thrombotic effect which is at least comparable to an activated partial thromboplastin time adjusted heparin infusion . - pubmed
The level of thrombin inhibition by efegatran , as measured by activated partial thromboplastin time , appeared to be more stable than with heparin . - pubmed
Thus , like other thrombin inhibitors , efegatran sulphate is easier to administer than heparin . - pubmed
In the present study , the effects of macrophage CSF ( M - CSF ) , G - CSF , GM - CSF and interleukin-3 ( IL-3 ) ( 10 ng / ml ) on the production of nitric oxide and prostaglandin E2 ( PGE2 ) by cartilage explants were examined over 24 and 48 h. The effects of these CSFs were also measured in combination with IL-1 beta ( 10 ng / ml ) . - pubmed
M - CSF , GM - CSF and IL-3 had no effect on nitrite production . - pubmed
However , both IL-1 beta and G - CSF caused a significant increase ( p & lt ; 0.05 ) in nitrite levels at 48 h. NG - L - arginine - methyl - ester was used to inhibit nitrite production induced by G - CSF and this implicated nitric oxide synthase activity . - pubmed
When G - CSF and IL-1 beta were used in a combined treatment , nitrite levels were significantly increased ( p & lt ; 0.05 ) at both 24 and 48 h. Both IL-3 alone and in combination with IL-1 beta caused elevated PGE2 production in this model . - pubmed
PGE2 levels were also significantly increased by stimulation with GM - CSF and IL-3 combined with IL-1 beta . - pubmed
These findings demonstrate that GM - CSF , G - CSF and IL-3 may induce changes in the production of inflammatory mediators such nitric oxide and PGE2 in cartilage chondrocytes . - pubmed
Serotonin 5 - HT ( 2 ) receptors activate local GABA inhibitory inputs to serotonergic neurons of the dorsal raphe nucleus . - pubmed
The purpose of the present study was to characterize the synaptic currents induced by bath - applied serotonin ( 5 - HT ) in 5 - HT cells of the dorsal raphe nucleus ( DRN ) and to determine which 5 - HT receptor subtypes mediate these effects . - pubmed
These effects were blocked by the GABA ( A ) receptor antagonist , bicuculline ( 10 microM ) and by the fast sodium channel blocker , TTX , suggesting that 5 - HT had increased impulse flow in local GABAergic neurons . - pubmed
A near maximal concentration of the selective 5 - HT ( 2A ) antagonist , MDL100,907 ( 30 nM ) , produced a large reduction ( approximately 70 % ) in the increase in IPSC frequency induced by 100 microM 5 - HT ; SB242,084 ( 30 nM ) , a selective 5 - HT ( 2C ) antagonist , was less effective ( approximately 24 % reduction ) . - pubmed
Combined drug application suppressed the increase in 5 - HT - induced IPSC frequency almost completely , suggesting involvement of both 5 - HT ( 2A ) and 5 - HT ( 2C ) receptors . - pubmed
Unexpectedly , the phenethylamine hallucinogen , DOI , a partial agonist at 5 - HT ( 2A / 2C ) receptors , caused a greater increase ( +334 % ) in IPSC frequency than did 5 - HT 100 microM ( +80 % ) . - pubmed
This result may be explained by an opposing 5 - HT ( 1A ) inhibitory effect since the selective 5 - HT ( 1A ) antagonist , WAY-100635 , enhanced the 5 - HT - induced increase in IPSCs . - pubmed
These results indicate that within the DRN - PAG area there may be a negative feedback loop in which 5 - HT induces an increase in IPSC frequency in 5 - HT cells by exciting GABAergic interneurons in the DRN via 5 - HT ( 2A ) and , to a lesser extent , 5 - HT ( 2C ) receptors . - pubmed
The Endologix device , currently being acquired by CR Bard , Incorporated , is a new , one - piece self - expanding polytetrafluoroethylene prosthesis that offers an alternative to current modular and fabric devices . - pubmed
He was found to have a subnormal cortisol level of 2.3 microg / dL at baseline , which increased to only 5.6 microg / dL 60 minutes after a 250 - microg corticotropin intravenous stimulation test . - pubmed
Binding of the C4BP - protein S complex to apoptotic cells was calcium - dependent and could be blocked with Abs directed against the phospholipid - binding domain in protein S. - pubmed
Annexin V , which binds to exposed phosphatidylserine on the apoptotic cell surface , could inhibit the binding of protein S. - pubmed
Both species showed increased ferric reductase activity upon Fe starvation , but they differed in the maximum rates . - pubmed
Changes in the distribution of saturated and unsaturated fatty acids ( FA ) at 30 and 37 degrees C were considered as one of possible causes of the TDS phenomenon . - pubmed
At identical temperatures , no statistically significant differences in the distribution of major FA ( iso-15:0 , anteiso-15:0 , 16:0 and 16:1 ) were registered between individual groups . - pubmed
BACKGROUND : The cardiovascular effects of angiotensin II are mediated by the angiotensin II type 1 receptor ( AGT1R ) ; one polymorphism of the AGT1R gene , A1166 - ->C , has been associated with hypertension . - pubmed
The hemodynamic response to angiotensin II is blunted in women compared to men , but interactions between gender , blood pressure , and AGT1R gene polymorphisms are unclear . - pubmed
In addition , the renal hemodynamic response to angiotensin II infusion is a function of both gender and the AGT1R genotype . - pubmed
Interaction of chlorisondamine with the neuronal nicotinic acetylcholine receptor . - pubmed
An epitope was found on the alpha2 - nicotinic isoform of the neuronal nicotinic acetylcholine receptor that would likely form salt bridges with quaternary ammonium compounds and a cation - pi interaction with the pi - cloud of an aromatic ring . - pubmed
The third complementarity - determining region ( CDR ) of the TCR alpha and beta chains forms loops that engage amino acid residues of peptides complexed with MHC . - pubmed
The joining of these gene segments is imprecise , leading to significant variability in the TCRbeta chain CDR3 loop length and amino acid composition . - pubmed
Moreover , biochemical analyses ( G6PD , CAT , SOD , GSH and MDA ) were determined in supernatant of kidney tissue . - pubmed
GLYCINE - SERUM INTERCONVERSION AND COLLAGEN BIOSYNTHESIS IN VIVO . - pubmed
POSSIBLE DIFFERENCE IN THE AMINO ACID SUPPLYING SYSTEM BETWEEN SKIN COLLAGEN AND SERUM ALBUMIN IN GUINEA PIGS . - pubmed
AIM : The aim of the present experiment was to study the effect of different chlorhexidine regimens on the number of bacteria in saliva , and on de novo plaque formation . - pubmed
In breast cancer patients , tamoxifen or aromatase inhibitors can be used for ovarian stimulation prior to oocyte or embryo cryopreservation . - pubmed
NE , NPY , ATP , and a P2 - receptor analogue were added before S2 . - pubmed
NPY ( 0.05 - 1.0 micromol / l ) concentration dependently inhibited acetylcholine release . - pubmed
This effect was prevented by the NPY - Y ( 2 )- receptor antagonist BIIE 0246 ( 0.1 micromol / l ) but not by the NPY - Y ( 1 )- receptor antagonist BIBP 3226 ( 10 micromol / l ) . - pubmed
Unprecedented olefin - dependent histidine - kinase inhibitory of zerumbone ring - opening material . - pubmed
After withdrawing tetracycline , the C - JNK fragment expression was induced and cell growth was dramatically inhibited 24 h later . - pubmed
Pre - incubation of cells with blocking agents of epithelial Na (+ ) channel ( ENaC ) for 15 min ( normal [ NaCl ] throughout ) caused Cu accumulation rates to increase by 40 - fold ( 100 micromol l(-1 ) phenamil ) , 21 - fold ( 10 micromol l(-1 ) CDPC ) or 12 - fold ( 2 mmol l(-1 ) amiloride ) when Cu ( o ) was 800 micromol l(-1 ) compared to those in drug - free controls . - pubmed
We conclude that Cu accumulation by intestinal cells is mainly Na(+)-insensitive and more characteristic of a pH- and K (+)-sensitive Ctr1 - like pathway than Cu uptake through ENaCs . - pubmed
Membrane - associated NADPH oxidase complexes catalyse the production of the superoxide anion radical from oxygen and NADPH . - pubmed
Simvastatin significantly improved CLP - induced mortality and AKI . - pubmed
Simvastatin attenuated CLP - induced tubular damage and reversed CLP - induced reduction of intrarenal microvascular perfusion and renal tubular hypoxia at 24 h. Simvastatin also restored towards normal CLP - induced renal vascular protein leak and serum TNF - alpha . - pubmed
Neither delayed simvastatin therapy nor TNF - alpha neutralizing antibody improved CLP - induced AKI . - pubmed
We present synthetic examples that validate our approach and show a comparison between existing segmentation techniques of paranasal sinus CT data . - pubmed
RESULTS : The levels of superoxide dismutase ( SOD ) and nitric oxide ( NO ) increased more significantly in the Shenfu group than in the control group ( P<0.05 ) . - pubmed
The levels of serum liver enzymes , hyaluronic acid ( HA ) , malondialdehyde ( MDA ) , tumor necrosis factor - alpha ( TNF - alpha ) , interleukin-1 ( IL-1 ) , endothelin - 1 ( ET-1 ) and liver cell apoptosis index were lower in the Shenfu group than in the control group ( P<0.05 ) . - pubmed
Bioisosteric modification of salvinorin A , a potent and selective kappa - opioid receptor agonist . - pubmed
Salvinorin A ( ( 2S,4aR,6aR,7R,9S,10aS , 10bR )- 2H - naphtho [ 2,1 - c ] pyran-7 - carboxylic acid , 9 -( acetyloxy )- 2 -( 3 - furanyl ) dodecahydro-6a,10b - dimethyl-4,10 - dioxo methyl ester , 1 , CAS 83729 - 01 - 5 ) has been shown to bind with high affinity and selectivity to the kappa - opioid receptor ( KOR ) as an agonist . - pubmed
It is now apparent that these and likely other additional molecules are required along with ORs to orchestrate the complex pattern of convergence and divergence that is unique to the olfactory system . - pubmed
The expression of the haemolysin also increased under iron - starvation conditions . - pubmed
Identification of the target compound was performed by ( 1 ) H NMR , ( 13 ) C NMR , two - dimensional NMR , electrospray ionization MS , IR and UV . - pubmed
Association of ABCB1 / MDR1 and OPRM1 gene polymorphisms with morphine pain relief . - pubmed
We focused on two such genes : ABCB1 / MDR1 , a major determinant of morphine bioavailability , and OPRM1 , which encodes for the mu - opioid receptor , the primary site of action for morphine . - pubmed
The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent SDS . - pubmed
The [ H - C ] and [ C - P ] bond lengths of HCP are set to their values at the linear equilibrium ground vibrational level of the molecule . - pubmed
, T<or=70 K. These quantities are significantly magnified in comparison with their counterparts for the HCP - He collisions . - pubmed
Interactions among type I and type II interferon , tumor necrosis factor , and beta - estradiol in the regulation of immune response - related gene expressions in systemic lupus erythematosus . - pubmed
Since interactions among TNF , IFNgamma , beta - estradiol ( E2 ) , and IFNalpha may regulate the expression of interferon - inducible ( IFI ) genes , stimulating and co - stimulating experiments were carried out on peripheral blood mononuclear cells followed by analysis using quantitative RT - PCR . - pubmed
A subset of 35 children underwent a meal test with blood draws to measure insulin and glucose . - pubmed
CYP2D6 and CYP3A5 genotypes were determined , and the plasma levels of RIS and 9 - OH - RIS were measured using high - performance liquid chromatography . - pubmed
The dose - normalized plasma concentrations of RIS , 9 - OH - RIS , and the active moiety were compared according to the CYP2D6 and CYP3A5 genotypes . - pubmed
The plasma levels of RIS ( 2.03 ng / mL per milligram for EMs vs 5.57 ng / mL per milligram for PMs , P & lt ; 0.001 ) and 9 - OH - RIS ( 5.06 ng / mL per milligram for EMs vs 0.22 ng / mL per milligram for PMs , P & lt ; 0.001 ) were significantly different among CYP2D6 genotype groups , but the CYP2D6 EMs ( 7.09 ng / mL per milligram ) and PMs ( 5.79 ng / mL per milligram ) did not show no difference in the levels of the active moiety ( P = 0.470 ) . - pubmed
CYP3A5 nonexpressors exhibited higher plasma concentrations of both RIS and 9 - OH - RIS than its expressors . - pubmed
In the case of 9 - OH - RIS , CYP3A5 nonexpressors exhibited significantly higher concentrations than CYP3A5 expressors ( 5.42 vs 3.51 ng / mL per milligram , P = 0.022 ) . - pubmed
In conclusion , both CYP2D6 and CYP3A5 genotypes affected plasma levels of RIS and 9 - OH - RIS , whereas the active moiety levels were influenced only by the CYP3A5 genotype but not by the CYP2D6 genotype . - pubmed
The VOC - responsive proteins contain ATP synthase CF1 , ribulose-1,5 - bisphosphate carboxylase / oxygenase , photosystem II light harvesting complex , and enolase , which are components of photosynthesis and carbohydrate metabolism . - pubmed
Based on RTS analysis for various temperatures , gate bias and optical power we determined the characteristics of these single photogenerated traps : the energy position within the silicon bandgap , capture cross section and the position within the Si / SiO(x = 1.5 ) interfaces . - pubmed
A novel method has been developed to detect two organic arsenic animal feed additives including roxarsone and p - arsanilic acid , as well as other arsenic species such as arsenite , dimethylarsinic acid , monomethylarsonic acid , arsenate , and 4 - hydroxyphenylarsonic acid , by using high - performance liquid chromatography coupled to an inductively coupled plasma mass spectrometer ( HPLC - ICP - MS ) . - pubmed
Liquid chromatography coupled to tandem mass spectrometry ( LC - MS / MS ) was used as an assistant tool to screen arsenobetain ( AsB ) in the feed samples by monitoring the reaction at m / z 179 - ->120 . - pubmed
The ion - pair reversed phase HPLC - ICP - MS method was then successfully applied to the speciation of arsenic in feedstuff and formula feed samples . - pubmed
Physiological parameters measured were pulse oxygen saturation ( SpO(2 ) ) , end - tidal CO ( 2 ) ( EtCO ( 2 ) ) , heart rate ( HR ) , and respiratory rate ( RR ) . - pubmed
Flavin mononucleotide - based fluorescent reporter proteins outperform green fluorescent protein - like proteins as quantitative in vivo real - time reporters . - pubmed
We identify more than 100 metabolites , including orsellinic acid derivatives , accumulating preferentially in the csnE mutant . - pubmed
Bronchoscopic Nd : YAG laser irradiation , argon plasma coagulation ( APC ) and drugs administration such as steroids and colchicines were reported to be effective in some patients . - pubmed
The multikinase inhibitor sorafenib ( Nexavar , Bayer ) , used in the treatment of renal and hepatocellular carcinoma , suppresses tumor angiogenesis and promotes autophagy in tumor cells . - pubmed
Tumor cell types that displayed high levels of cell killing after combination treatment showed elevated levels of AKT , p70 S6 K , and/or phosphorylated mTOR , in addition to class III receptor tyrosine kinases such as platelet - derived growth factor receptor beta and VEGF receptors , known in vivo targets of sorafenib . - pubmed
Importantly , slices responded to the application of E-4031 , chromanol and 4 - aminopyridine , three potassium channel blockers known to affect action potential duration , with an increase in FPD . - pubmed
In vitro selection of variants resistant to beta - lactams plus beta - lactamase inhibitors in CTX - M beta - lactamases : predicting the in vivo scenario ? - pubmed
The therapeutic options for CTX - M - carrying isolates are scarce , forcing the reexamination of the therapeutic possibilities of beta - lactams plus beta - lactamase inhibitors ( BBLIs ) . - pubmed
Fourteen CTX - M beta - lactamases belonging to the three most representative clusters ( CTX - M-1 , -2 , and -9 ) and the two main phenotypes ( cefotaxime resistance and cefotaxime - ceftazidime resistance ) were studied . - pubmed
The most frequently recovered mutation was S130 G , which conferred the highest resistance levels to BBLIs ( reaching 12 mug / ml for amoxicillin - clavulanate and 96 mug / ml for piperacillin - tazobactam when acquired by CTX - M-1 cluster enzymes ) . - pubmed
The glmS ribozyme tunes the catalytically critical pK(a ) of its coenzyme glucosamine-6 - phosphate . - pubmed
Here we used Raman crystallography directly to determine the pK(a ) 's of GlcN6P bound to the glmS ribozyme . - pubmed
Moreover , the anionic glmS ribozyme in effect stabilizes the neutral amine of GlcN6P by lowering its pK(a ) . - pubmed
Lipid peroxidation , glycogen content of liver and skeletal muscles , insulin , superoxide dismutase ( SOD ) , catalase ( CAT ) , glutathione ( GSH ) , oxidized glutathione ( GSSG ) levels were determined . - pubmed
Chloroform extract from AI shows increased levels of SOD , GSH , GSSG and CAT , hepatic glycogen content , glucose-6 - phosphatase and insulin plasma levels , which also decreased the glucokinase ( GK ) , lipid peroxidation and insulin resistance . - pubmed
Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration . - pubmed
Recent studies demonstrated that proprotein convertase subtilisin / kexin type 9 ( PCSK9 ) plays a critical role in circulating LDL cholesterol concentrations . - pubmed
Rapid Disruption of Cellular Integrity of Zinc - treated Astroglia Is Regulated by p38 MAPK and Ca - dependent Mechanisms . - pubmed
Among the early cellular events occurring in zinc - treated astroglia , strong activation of p38 MAPK and JNK was evident . - pubmed
Although inhibitors of p38 ( SB203580 and SB202190 ) or JNK ( SP600125 ) did not protect zinc - insulted astroglia from cell death , the p38 inhibitors , but not the JNK inhibitor , suppressed actin filament and cell morphology disruption . - pubmed
The Ca ( 2 + ) ionophore , A23187 , also suppressed actin filament and cell morphology disruption , but not cell death , of zinc - insulted astroglia . - pubmed
However , A23187 did not inhibit p38 MAPK activation in zinc - treated astroglia . - pubmed
Together these results suggest that zinc influx in astroglia results in rapid loss of the morphologic integrity via mechanisms regulated by p38 kinase and/or Ca ( 2 + ) signaling . - pubmed
The core electron temperature ( T(e0 ) ) of neutral beam heated plasmas is determined in TJ - II stellarator by using soft x ray detectors with beryllium filters of different thickness , based on the method known as the foil absorption technique . - pubmed
Barium swallow and CT scan of the chest were inconclusive . - pubmed
To evaluate chemical oxygen demand ( COD ) removal efficiency and granule formation of the novel reactor ( R1 ) , in comparison a conventional SBAR ( R2 ) was operated under similar conditions during the experimental period . - pubmed
Experiments were done at different organic loading rates ( OLRs ) ranging from 0.6 - 4.5 kg COD / m3.d for R1 and from 0.72 - 5.4 kg COD / m3.d for R2 . - pubmed
In the second part of the experiment , the efficiency of removal of a toxic substance by aerobic granules was investigated using aniline as a carbon source with a concentration in the range 1.2 - 6.6 kg COD / m3.d and 1.44 - 7.92 kg COD / m3.d in R1 and R2 , respectively . - pubmed
Increased SIRT1 expression in HUVECs by syringaresinol treatment delayed cellular senescence and improved various markers of endothelial functions in a FOXO3 dependent manner . - pubmed
The MTT and wound - healing assays showed that the TF - siRNA substantially inhibited the proliferation and migration of HUVECs . - pubmed
Catabolism of triglyceride - rich lipoproteins creates free fatty acids that are readily taken up by endothelial cells , and , likely through the action of acyl - CoA synthetases , exacerbate inflammatory processes . - pubmed
A novel hollow urchin - like alpha - FeOOH hierarchical structure was fabricated via a one - pot hydrothermal procedure without using any templates , surfactants or organic raw materials . - pubmed
Hospitalization , use of sirolimus , low hemoglobin level , and use of renin - angiotensin system inhibitors were independently associated with these events . - pubmed
Conversely , over the 3 - year study , the number of positive Epstein - Barr virus viremias over the number of Epstein - Barr virus polymerase chain reactions performed was significantly higher in the belatacept group ( 76 % vs 50 % ; P = .047 ) . - pubmed
There was a greater age- , sex- and ethnicity - adjusted odds of AN among those : overweight ( 3.98 ; 2.10 , 7.55 ) or obese ( 8.31 ; 3.84 , 18.00 ) versus normal BMI ; centrally obese ( 4.72 ; 2.65 , 8.43 ) ; with history of hypertension ( 2.19 ; 1.27 , 3.79 ) or history of hypercholesterolemia ( 1.72 ; 1.02 , 2.90 ) , but there was no evidence of this demographic - adjusted association ( p & gt ; 0.4 ) between AN and history of previous MI or CVA , family history of T2DM , T2DM treatment regimen , duration of T2DM or random blood glucose . - pubmed
Opening and closing of the cystic fibrosis transmembrane conductance regulator are controlled by ATP binding and hydrolysis by the cytoplasmic nucleotide - binding domains . - pubmed
Renal function was assessed by measurement of sCysC levels and albuminuria ( calculated as the urine albumin - to - creatinine ratio ( uACR ) ) . - pubmed
The presence of EMH was evaluated based on steady - state free precession sequences ; cardiac and liver iron overloads were quantified using a multiecho T2 * approach ; cardiac function parameters and pulmonary diameter were quantified using the steady - state free precession sequences ; and myocardial fibrosis was evaluated using the late gadolinium enhancement technique . - pubmed
Spiral Bragg grating waveguides for TM mode silicon photonics . - pubmed
We demonstrate spiral Bragg grating waveguides ( BGWs ) on the silicon - on - insulator ( SOI ) platform for the fundamental transverse magnetic ( TM ) mode . - pubmed
Cell cycling and apoptosis were assessed by propidium iodide ( PI ) and annexin V - FITC / PI flow cytometry , respectively . - pubmed
In this study we characterize the anti - glioma effect of VCN-01 , an improved hyaluronidase - armed pRB - pathway - selective oncolytic adenovirus that has proven safe and effective in the treatment of several solid tumors . - pubmed
METHODS : Six reagents Clearfil(TM ) SE Bond primer ( SP ) , Clearfil(TM ) SE Bond adhesive ( SA),Clearfil(TM ) Protect Bond primer ( PP ) , which contained antibacterial monomer methacryloyloxydodecylpyridinium bromide ( MDPB ) , ClearfilTM Protect Bond adhesive ( PA ) , positive control chlorhexidine acetate [ CHX , 1 % ( mass fraction ) ] , and negative control phosphate buffer solution ( PBS ) were selected . - pubmed
mutans ( P<0.05 ) ; SP and PP showed stronger antibacterial effect than PA , SA and CHX ( P<0.05 ) . - pubmed
CHX , SP and PP presented inhibition zones , while PBS , SA and PA did not . - pubmed
Compared with PBS , the CHX , SP and PP could lower the ratio of the live to dead bacteria significantly ( P<0.05 ) . - pubmed
However , systemic letrozole treatment did not augment the stimulus - evoked release of the neuropeptide calcitonin gene - related peptide ( CGRP ) from spinal cord slices , suggesting that the enhanced nociceptive responses were not secondary to an increase in peptide release from sensory endings in the spinal cord . - pubmed
Contribution of Matrix Metallopeptidase-1 Genotypes , Smoking , Alcohol Drinking and Areca Chewing to Nasopharyngeal Carcinoma Susceptibility . - pubmed
In addition , individuals carrying the 1 G allele at MMP-1 promoter -1607 were less susceptible to NPC ( OR=0.73 ; 95%CI=0.59 to 0.96 , p=0.0418 ) after adjustment for age , gender , cigarette , alcohol and areca consumption . - pubmed
CONCLUSION : The 1G/1 G genotype of MMP-1 promoter -1607 may independently have a protective effect on NPC risk , without interaction with behavioral factors , including cigarette , alcohol and areca consumption . - pubmed
The rapid uptake of 68Ga prostate - specific membrane antigen HBED - CC positron emission tomography ( PSMA PET ) imaging for prostate cancer staging has led to concerns regarding its specificity , with uptake in both malignant and nonmalignant tissues . - pubmed
But also today , EPP is still a valuable option in selected cases and the entire treatment concept requires a nuanced view , which will be discussed in this review . - pubmed
Selectivities of 70 - 80 % for C2 and C3 products with a hydrocarbon ratio of C>=2 / CH4 significantly greater than 100 have been observed with several additives . - pubmed
The complete repertoire of endothelial functions elicited by FGD5 , a guanine nucleotide exchange factor activating the Rho GTPase Cdc42 , has yet to be elucidated . - pubmed
Furthermore , depletion of FGD5 resulted in accelerated VEGFR2 degradation , which was reverted by lactacystin - mediated proteasomal inhibition . - pubmed
Editorial SI : Octamethylcyclotetrasiloxane . - pubmed
Retigabine ( RTG ) is a first - in - class antiepileptic drug that suppresses neuronal excitability through the activation of voltage - gated KCNQ2 - 5 potassium channels . - pubmed
To elucidate how the retigabine binding site is coupled to changes in voltage sensing , we used voltage - clamp fluorometry to track conformational changes of the KCNQ3 voltage - sensing domains ( VSDs ) in response to voltage , retigabine , and PIP2 . - pubmed
We identify a polybasic motif in the proximal C terminus of retigabine - sensitive KCNQ channels that contributes to VSD - pore coupling via PIP2 , and thereby influences the unique gating effects of retigabine . - pubmed
The synthesis of phosphatidylcholine ( PC ) , the most abundant cellular phospholipid , occurs redundantly via the ER - localized Kennedy pathway and a pathway that traverses the ER and mitochondria via membrane contact sites . - pubmed
The basis of the ER - mitochondrial PC synthesis pathway is the exclusive mitochondrial localization of a key pathway enzyme , phosphatidylserine decarboxylase Psd1 , which generates phosphatidylethanolamine ( PE ) . - pubmed
Renal cells with upregulated miR-146 had lower plasma levels of blood urea nitrogen ( BUN ) and creatinine , decreased apoptosis and active caspase-3 protein expressions . - pubmed
In the former , P2Y and possibly some P2X receptors - ion channels regulate insulin secretion , but it is still debated whether excessive ATP can via P2X receptors impair beta - cell function directly or whether cell damage is due to an excessive systemic release of cytokines . - pubmed
The Detection of Uterine Leiomyoma ( Fibroid ) Calcifications on 18F - NaF PET / CT . - pubmed
We are presenting a calcified uterine leiomyoma in a 61 - year - old woman as incidentally detected on F - NaF PET / CT scanning . - pubmed
The mature XynC has 454 amino acid residues and a calculated molecular weight of 49 240 . - pubmed
It liberates a set of oligosaccharides , which have been detected by thin - layer chromatography , thus indicating that it is an endo - acting xylanase . - pubmed
Then to catalyze the zeolite Y wash - coated monolith with the cobalt precursor , the dip coating technique was used . - pubmed
Though Pusa Basmati1 ( PB1 ) showed higher tolerance to As , IR64 overcomes As toxicity more efficiently than PB1 in the presence of Se , which highlights that IR64 is a better performer in the presence of Se . - pubmed
Our EGA - IAMS results obtained from the thermal decomposition of vitamin C were compared with a previous study employing pyrolysis - gas chromatography - mass spectrometry ( Pyr - GC - MS ) . - pubmed
The observed quantitative and qualitative differences of the pyrolysis products obtained by the two techniques ( EGA - IAMS vs. Pyr - GC - MS ) are in part due to the difference in transportation time of the products out of the pyrolysis chamber . - pubmed
PDH is central to metabolism because it steers most of the glucose - derived flux into the TCA cycle . - pubmed
Here , we provide evidence that the RNA editing enzyme adenosine deaminase acting on RNA 2 ( ADAR2 ) is mislocalized in C9orf72 repeat expansion mediated ALS / FTD . - pubmed
ADAR2 is responsible for adenosine ( A ) to inosine ( I ) editing of double - stranded RNA , and its function has been shown to be essential for survival . - pubmed
The clinical effectiveness rate in the TEC group was significantly higher than that in the CEF group ( p<0.05 ) . - pubmed
There was no difference in the pathology grade between the two groups ( p>0.05 ) ; however , the pathological effective rate in the TEC group was significantly higher than that in the CEF group ( p<0.05 ) . - pubmed
While there was no difference in the relative expression of ANXA3 between the two groups before chemotherapy , the relative expression of ANXA3 in the TEC group was lower than that in the CEF group after NAC ( p<0.05 ) . - pubmed
In this study , we investigated the role of TRIM33 in myeloid cells during dextran sulfate sodium ( DSS)-induced colitis . - pubmed
Methotrexate ( MTX ) was administered in a 5 - day regimen : 0.3 - 0.5mg / kg intravenously ( IV ) daily for 5 days every 2 weeks ( maximum 25 mg per dose ) . - pubmed
Actinomycin D ( ActD ) was administered 1.25mg / m2 pulsed IV every 2 weeks . - pubmed
Up - regulation of sirtuin 1 induced by resveratrol also diminished the TNF - alpha - induced inflammatory response in BMSCs . - pubmed
Thus , betaOHB may regulate epigenetics by modulating HDACs . - pubmed
The aim of this study was to investigate whether betaOHB enhances cisplatin - induced apoptosis in hepatocellular carcinoma ( HCC ) cells by modulating activity and/or expression of HDACs . - pubmed
We found that betaOHB significantly enhanced cisplatin - induced apoptosis and cleavage of caspase-3 and - 8 in HCC cells . - pubmed
Acid sphingomyelinase ( ASM ) is a lysosomal hydrolase that degrades sphingomyelin into ceramide and phosphocholine . - pubmed
The Na / H exchange regulatory factor 1 ( NHERF1 ) , a member of a PDZ scaffolding protein family , was first identified as an organizer of membrane - bound protein complexes composed of hormone receptors , signal transduction pathways , and electrolyte and mineral transporters and channels . - pubmed
, 0 , 120 , and 240 mg kg-1 of soil ( for pot experiment ) and 0 , 300 , and 600 kg ha-1 ( for field experiment ) regarded as LSe , MSe and HSe , respectively and two Si levels i.e. - pubmed
, 0 and 60 mg kg-1 of soil ( for pot experiment ) and 0 and 150 kg ha-1 ( for field experiment ) regarded as -Si and + Si , respectively . - pubmed
Using different behavioral tests , we found that a single intravenous injection of methylene blue ( MB , 1 mg / kg ) 30 min after the injury reduced the impairment of the motor functions of the limbs from 7 to 120 days after TBI . - pubmed
We observed that protease complexed with Cyclocytidine hydrochloride ( anti - viral and anti - cancer ) , Trifluridine ( anti - viral ) , Adonitol , and Meropenem ( anti - bacterial ) , and Penciclovir ( anti - viral ) bound with a good docking score ranging from -6.8 to -5.1 ( Kcal / mol ) . - pubmed
Further , NSP10 / NSP16 methyltransferase complexed with Telbivudine , Oxytetracycline dihydrate ( anti - viral ) , Methylgallate ( anti - malarial ) , 2 - deoxyglucose and Daphnetin ( anti - cancer ) from the docking score of -7.0 to -5.7 ( Kcal / mol ) . - pubmed
The EDC values correlated well ( R2 = 0.93 ) with the 24 h Cl2 demand of the model compounds ( except for phenol and tannic acid ) . - pubmed
Our results showed that azosemide and torasemide caused a significant reduction in brain natriuretic peptide ( BNP ) level . - pubmed
Torasemide also caused a significant decrease in collagen volume fraction ( CVF ) and edema . - pubmed
We showed that KY1044 induced sustained depletion of ICOShigh T cells but was also associated with increased secretion of pro - inflammatory cytokines from ICOSlow TEFF cells . - pubmed
KY1044 demonstrated monotherapy antitumor efficacy and improved anti - PD - L1 efficacy . - pubmed
In summary , we demonstrated that using KY1044 , one can exploit the differential expression of ICOS on T cell subtypes to improve the intratumoral immune contexture and restore an antitumor immune response . - pubmed
LRAT , retinoic acid , renin angiotensin system ( RAS ) biomarkers , and the structure and function of the heart for SHRs were measured . - pubmed
Compared with VAN - treated SHRs , LRAT , retinoic acid receptor alpha ( RARalpha ) , ACE2 , and Ang ( 1 - 7 ) protein expression levels were decreased , while ACE and AT1R expression levels were increased in VAD SHRs . - pubmed
Catalytic Synthesis of Potassium Acyltrifluoroborates ( KATs ) from Boronic Acids and the Thioimidate KAT Transfer Reagent . - pubmed
We report the synthesis of potassium acyltrifluoroborates ( KATs ) by a palladium - catalyzed cross - coupling of boronic acids and the thioimidate KAT transfer reagent . - pubmed
The combination of widely available aryl - and vinylboronic acids with commercially available thioimidate 1 using catalytic Pd II and a Cu II additive enables the preparation of KATs in high yields and with good functional group tolerance . - pubmed
The cross - coupling can be telescoped into the one - pot synthesis of amides and a -aminotrifluoroborates by exploiting the unique chemistry of KATs and their trifluoroborate iminium ( TIM ) derivatives . - pubmed
One hundred eighty trough levels were first measured , and their median value was 16.2 mug / mL ( range , 2.3 - 100 mug / mL ) and the median interval between initial teicoplanin injection and 1st trough level was 96.5 hours ( range 47.6 - 179.3 hours ) . - pubmed
Deciphering the Molecular Details of the Lipoxin Formation Mechanism in the 5 ( S ) , 15 ( S )- DiHpETE Biosynthetic Pathway Catalyzed by Reticulocyte 15 - Lipoxygenase-1 . - pubmed
The biosynthesis of lipoxins is catalyzed by several lipoxygenases ( LOXs ) from arachidonic acid . - pubmed
In this paper , we have combined molecular dynamics ( MD ) simulations and quantum mechanics / molecular mechanics ( QM / MM ) calculations to analyze how reticulocyte 15 - LOX-1 catalyzes the production of lipoxins from 5 ( S ) , 15 ( S )- diHpETE . - pubmed
Conversely , the oxygenation of 5 ( S ) , 15 ( S )- diHpETE at C14 is feasible because there is an open channel connecting the protein surface with this carbon atom , and the energy barrier for oxygen addition through this channel is small . - pubmed
The analysis of the following steps of this mechanism , leading to the corresponding hydroperoxide at the 15 - LOX-1 active site , indicates that the oxygenation mechanism will lead to the formation of lipoxinB4 after the final action of a reductase . - pubmed
In contrast , our calculations are in agreement with experiments that lipoxinA4 can not derive from 5 ( S ) , 15 ( S )- diHpETE by either of the two proposed mechanisms and that 5 ( S ) , 15 ( S )- diHETE is not an intermediate of lipoxin biosynthesis catalyzed by 15 - LOX-1 . - pubmed
Pharmacogenetic Variants in the DPYD and TYMS Genes are Clinically Significant Predictors of Fluoropyrimidine Toxicity : Are We Ready for Use in our Clinical Practice . - pubmed
Dantrolene Potentiates the Antineoplastic Effect of Sorafenib in Hepatocellular Carcinoma via Targeting Ca + 2 / PI3 K Signaling Pathway . - pubmed
OBJECTIVE : This study was conducted to evaluate the role of dantrolene as a possible antineoplastic agent in HCC , and in chemo - sensitization of sorafenib via targeting Ca + 2 / PI3 K pathway . - pubmed
RESULTS : Dantrolene exhibited synergistic effect when used in combination with sorafenib compared to either drug alone ( p < 0.05 ) through decreasing p53 , VEGF , MMP-9 , Cyclin D1 , and Ki-67 . - pubmed
In addition , dantrolene was evidenced to have an inhibitory effect on Ca + 2 / PI3 K pathway that mediates its antineoplastic action when used alone or in combination with sorafenib . - pubmed
CONCLUSION : Dantrolene exerted antineoplastic effect as well as augmented sorafenib antineoplastic activity via intervention of Ca + 2 / PI3 K pathway , manifested by ameliorating angiogenesis , apoptosis , proliferation and metastasis . - pubmed
Although insulin , by itself , had a stimulatory effect on [ 125I ] UdR incorporation in cultured prostate , the magnitude of the response did not differ in the 6- and 26 - week - old prostate tissue . - pubmed
This spontaneous surge in activity during culture tended to mask the stimulatory effects of insulin and testosterone at concentrations of 3 mug / ml and 10(-7 ) mol / l respectively . - pubmed
They do not modify the prostate 5alpha - reductase activity ; however , they do inhibit the binding of androgens to their receptors . - pubmed
All these fractions , except one casein kinase of the cytosol , have been studied with respect to protein and nucleotide specificity , effect of salts and of cyclic nucleotides , sedimentation , etc . - pubmed
The content of CGRP-2 , substance P , and somatostatin in adenomas correlated directly with that of parathyroid hormone . - pubmed
In addition , concentrations of VIP versus substance P and somatostatin versus CGRP-2 in adenomas were directly correlated . - pubmed
In hyperplasias , content of substance P correlated directly with that of gastrin - releasing peptide . - pubmed
Thus , dopamine effectively inhibited PRL secretion during the later lactational stage , but could not do so during the earlier stage when there were mechanisms other than dopamine stimulating PRL secretion . - pubmed
The mechanism of ovulation blockade during lactation thus seems to shift from being progesterone independent to progesterone dependent at a similar period when the neuroendocrine control of PRL secretion shifts from dopamine independent to dependent . - pubmed
Endonuclease V recognizes ultraviolet radiation ( UVR)-induced pyrimidine dimers and produces single - strand breaks adjacent to the dimers . - pubmed
When removal of UVR - induced photoproducts was initiated by endogenous excision repair mechanisms , an average of 38 nucleotides were replaced per dimer removed , as determined by bromouracil photolysis ; denV - initiated excision repair , on the other hand , resulted in removal of an average of 6 nucleotides per dimer repaired . - pubmed
The enhanced pyrimidine dimer repair capabilities conferred by denV gene expression did not appear to improve post - UVR survival . - pubmed
Internally trapped cytochrome c can be reduced by the membrane - permeable reductants 2,3,5,6 - tetramethyl - p - phenylenediamine ( DAD ) or N , N , N ' , N '- tetramethyl - p - phenylenediamine ( TMPD ) . - pubmed
Respiration on internal cytochrome c generated a membrane potential of 53 mV ( positive inside ) and a pH gradient of 0.2 ( acid inside ) as monitored by the optical probes oxonol V and pyranine , respectively . - pubmed
The steady - state internal acidification induced by turnover of the internally facing enzyme is probably maintained by both cytochrome oxidase proton translocation and a TMPD+/H+ antiport present in these vesicles [ Cooper , C. E. , & amp ; Nicholls , P. ( 1987 ) FEBS . - pubmed
The heart to lung ratio both at rest and exercise for 99mTc - MIBI is significantly higher than 201Tl ( P less than 0.01 and less than 0.001 respectively ) . - pubmed
Analysis with restriction enzymes shows that both methyl and ethyl groups influence the restriction patterns of the enzymes HpaII and MspI that recognize specific endogenous DNA methylation . - pubmed
Intravenous infusion of natural peptide YY at 1 and 0.2 microgram / kg / hr caused 60 and 40 % inhibition of secretin- and cholecystokinin - stimulated secretion , respectively , and 40 - 50 and 20 - 40 % inhibition of intraduodenal oleate stimulated secretion , respectively . - pubmed
A significant but transient decrease in the plasma cholecystokinin level was observed in response to peptide YY under oleate stimulation . - pubmed
The levels of both factor VIII : C and factor VIIIR : Ag increased , the former less than the latter resulting in a rise of the FVIIIR : Ag / FVIII : C ratio . - pubmed
FVIIIR : Ag / FVIII : C ratio was increased both in mild ( n = 7 ) and severe ( n = 9 ) preeclampsia , whereas beta - thromboglobulin was increased and AT III was decreased only in severe preeclampsia . - pubmed
Platelet count and lifespan , plasma and serum thromboxane B2 as well as FVIII : C were normal in severe preeclampsia . - pubmed
Stable acyl - derivatives of tissue - type plasminogen activator . - pubmed
Injections of morphine - HCl ( MP ) ( 25 , 50 , 100 and 150 micrograms / g ) were administered and the DR was examined at 2 , 7.5 , 15 , 30 , 45 and 75 min post - injection . - pubmed
( b ) A 100 micrograms / g dose of MP caused a 50 % response inhibition with an injection - shock interval of 30 minutes , but no inhibition occurred when the same dose was administered with 1.6 micrograms / g of naloxone - HCl , suggesting that MP acts through an opiate receptor . - pubmed
After training , each patient exceeded FVC with one or more resistors ; " optimum " FVCPEP was 124 + /- 16 % FVC at a SEP of 61 + /- 21 cm H2O. - pubmed
Hypoxia induced a decline in cardiac contractile force , a decrease in myocardial high - energy phosphates and a release of ATP metabolites and creatine phosphokinase from the perfused heart . - pubmed
Upon reoxygenation the rate of release of ATP metabolites subsided , but no appreciable recovery of the loss of contractile force and the reduction of myocardial ATP content was seen , and the release of creatine phosphokinase was increased further . - pubmed
Both analyses showed low activity of the enzymes studied and a decrease of the androgen level in E2 treated cultures . - pubmed
When the dose of estradiol preceded LH addition the androgen response to LH was attenuated . - pubmed
Recent studies suggest keyroles for terminal components of complement system , products of lipoxygenase pathway of arachidonic acid and oxygen radicals - all of which are not readily influenced by available therapeutic modulaties . - pubmed
The effect of dexamethasone on corneal neovascularization induced by epidermal growth factor . - pubmed
The divalent cation - selective ionophore A23187 has been used to characterize cytoplasmic Ca and Mg buffering , Ca2 + -pump parameters and the properties of a Ca2 + - activated K +- channel in intact red cells . - pubmed
Phosphatidic acid , phosphatidyl ethanolamine , phosphatidyl serine , phosphatidyl inositol , phosphatidyl glycerol and cardiolipin were found to activate greatly the cathepsin D. - pubmed
The ABH " blood - group " antigens are present on differentiated epidermal cell surfaces and identify the grafted epidermis according to the blood groups of the donor . - pubmed
3 The SSPC of PL before exposure to DPH varied from 1.2 to 4.4 microgram ml-1 ( mean + /- s.e.mean 2.7 + /- 0.13 ) , and following exposure from 1.1 to 3.8 microgram ml-1 ( mean + /- s.e.mean 2.2 + /- 0.13 ) . - pubmed
4 SSPCs of AA and of PL decreased significantly during DPH administration ( P less than 0.01 for AA , P less than 0.001 for PL ) . - pubmed
6 Similarity was observed between the changes in plasma levels of AA and PL following DPH ingestion . - pubmed
Pericytes and endothelial cells were studied by immunohistochemistry , lectin labeling , electron microscopy , 3H - thymidine labeling followed by autoradiography , and time - lapse video microscopy . - pubmed
We report here that there is an increase of tyrosine kinase activity immunoprecipitable with anti - phosphotyrosine antibodies specifically in response to calcium -- and a number of other divalent cations -- within 2 min of exposure . - pubmed
A second tyrosine kinase activity is induced in response to both calcium and TPA , and has been identified as fyn , a nonreceptor tyrosine kinase of the src family . - pubmed
fyn activation is induced in keratinocytes within 6 hr of calcium exposure , but already within 2 min of TPA treatment . - pubmed
Cortactin , a p80 - 85 substrate of src- and fyn - related kinases that localizes with actin at cell adhesion sites , is increasingly tyrosine phosphorylated in calcium- and TPA - induced differentiation , with a time course which parallels that of fyn activation . - pubmed
Autoregulation of glucocorticoid receptor ( GR ) concentration in vivo may be an important determinant of steroid sensitivity . - pubmed
Receptor and TAT message levels were determined by quantitative Northern hybridization . - pubmed
Methylprednisolone plasma kinetics showed a half - life of 0.6 h. Receptor occupancy occurred rapidly and cytosolic GR reappeared over 2 - 12 h. TAT activity rose between 2 and 6 h and then dissipated . - pubmed
In contrast , TAT message induction occurred with a sharp peak ; maximal induction occurred between 5 - 6 h and return to baseline at approx . - pubmed
This study shows that unlike TAT induction , GR message repression in vivo does not require continual presence of hormone . - pubmed
Activators of protein kinase C mimic serotonin - induced modulation of a voltage - dependent potassium current in pleural sensory neurons of Aplysia . - pubmed
In the pleural mechanoafferent sensory neurons of Aplysia , serotonin ( 5 - HT)-induced spike broadening consists of at least two components : a cAMP and protein kinase A ( PKA)-dependent , rapidly developing component and a protein kinase C ( PKC)-dependent , slowly developing component . - pubmed
Voltage - clamp experiments were conducted to identify currents that are modulated by PKC and thus may contribute to the slowly developing component of 5 - HT - induced spike broadening . - pubmed
We compared the effects of phorbol esters , activators of PKC , on membrane currents with those of 5 - HT . - pubmed
Previous work has shown that these effects represent , in part , the modulation of a large , voltage - dependent K + current ( IK , V ) by 5 - HT . - pubmed
Active phorbol esters mimicked some of the actions of 5 - HT on membrane currents in that they slowed activation and inactivation kinetics of current responses to voltage - clamp pulses more positive than 0 mV. - pubmed
Because inactive phorbols did not mimic the actions of 5 - HT , the effects of active phorbol esters appeared to be PKC specific . - pubmed
In addition , preexposure of the sensory neurons to active phorbol esters appeared to occlude the modulatory actions of 5 - HT on IK , V. Thus it is likely that modulation of IK , V by 5 - HT is mediated , at lease in part , by PKC . - pubmed
To further characterize which currents were modulated by PKC , low concentrations of tetraethylammonium ( TEA , 2 mM ) were used to block Ca ( 2 +)-activated K + current ( IK , Ca ) . - pubmed
Low TEA partially blocked the phorbol ester - induced increase of the outward current at the end of voltage - clamp pulses . - pubmed
These results agreed with previous reports that activation of PKC enhanced a fast component of IK , Ca in these sensory neurons . - pubmed
Low TEA , however , did not affect phorbol ester - induced decrease of the outward current at the beginning of pulse , where the predominant current is IK , V , which is less sensitive to TEA. - pubmed
In addition , biochemical data including fasting plasma glucose , hemoglobin ( HD)Alc , cholesterol , triacylglycerol , high- and low - density lipoprotein cholesterol , uric acid , blood urea nitrogen ( BUN ) and creatinine ( Cr ) were studied . - pubmed
Moreover , topoisomerase activities were not affected by docetaxel . - pubmed
The acetylcholine - related enzymes acetylcholinesterase ( AChE ) and butyrylcholinesterase ( BuChE ) were measured in plasma , erythrocytes , platelets and lymphocytes . - pubmed
Electron microscopic immunolocalization of GABA and glutamic acid decarboxylase ( GAD ) in cerebellar capillaries and their microenvironment . - pubmed
The objective of the present study was to determine the influence of bombesin , a potent mitogen , supplemented alone or in combination with insulin , transferrin and/or EGF . - pubmed
When compared with 5 day controls ( L-15 medium only ) , bombesin generated a maximal though weak effect on DNA synthesis at a concentration of 0.3 nM , corresponding to a stimulation index ( SI ) of 22 % . - pubmed
When combined with either transferrin or EGF , or with transferrin plus EGF , bombesin did not alter the SI of individual factors . - pubmed
Insulin , in turn , greatly increased DNA synthesis ( SI = 169 % ) , while bombesin strongly potentiated this effect ( SI = 275 % ) . - pubmed
When added as a third factor , bombesin further potentiated the effectiveness ( SI = 338 % ) of the combination insulin plus transferrin . - pubmed
In a test of working memory using a three - panel runway task , the 5 - HT1A receptor agonist , 8 - hydroxy-2 -( di - n - propylamino ) tetralin ( 8 - OH - DPAT ) , at 1.0 mg / kg i.p . - pubmed
The effect of intrahippocampal 8 - OH - DPAT ( 10 micrograms / side ) on working memory was blocked by the 5 - HT1A receptor antagonist , ( -)-propranolol , at 5 mg / kg i.p . - pubmed
S-100 - positive neurons are distinguished by spontaneous discharge activity in a beating or bursting mode and a prominent Ca ( 2 +)-activated potassium outward current . - pubmed
The intent of this retrospective study was to perform a risk - benefit analysis between treatment strategies with bolus steroids only versus anti - thymocyte globulins ( RATG ; 1.5 mg / kg q 4 days ) . - pubmed
Heparin inhibitable lectins are physiologically important because of their interactions with extracellular matrix and with other cell surface glycoconjugates . - pubmed
When proteins were loaded on to a phosphate - buffered saline ( PBS ) equilibrated column and eluted with salt gradient , only one lectin peak was obtained . - pubmed
Lectin activity was stimulated by Mn ++ ; amino acid composition was different from other known lectins . - pubmed
The lectin was particularly rich in acidic amino acids . - pubmed
Hepatic cytochrome P-450 enzymes mediate at least two important biotransformation pathways of codeine and ethylmorphine starting with either N - demethylation or O - dealkylation , producing polar metabolites which are then subsequently glucuronidated . - pubmed
Butyrate and colonocytes : Bax to the front . - pubmed
A truncated version of the gene which lacks 381 bp of 5'-sequence also has pullulanase activity and it appears that the amino - terminal portion of the gene is not essential for either activity or thermostability . - pubmed
Amino acid sequence comparisons with other published amylases and pullulanases showed that it possesses homology to the four key regions common to these enzymes . - pubmed
The effect of age on cholinesterase activity , the infusion rate depressing twitch tension by 90 % ( IR90 ) , C90 , Cl ( trans - trans ) , and Cl ( cis - trans ) was determined using linear regression . - pubmed
mGluR activation reveals a tonic NMDA component in inflammatory hyperalgesia . - pubmed
After auto - radiographic in situ hybridization , counting silver grains over cells was used to quantitate and compare levels of expression of the N - methyl - D - aspartate receptor-2 subunits . - pubmed
Thus , these data provide clear functional support for the hypothesis that the C3H / HeJ defect exerts a dominant negative effect on LPS sensitivity ; however , expression of a normal Toll - like receptor 4 molecule is apparently not required . - pubmed
Labeling of adjacent sections for enkephalin or substance P showed that the dynorphinergic terminals overlapped those for substance P in the medial half of the internal pallidal segment , but showed only slight overlap with enkephalinergic terminals in the external pallidal segment . - pubmed
Telmisartan did not have consistent effects on either plasma norepinephrine or plasma atrial natriuretic peptide levels , although it did cause significant increases in both plasma renin activity and angiotensin II levels at higher doses . - pubmed
CONCLUSIONS : The acute administration of the angiotensin II antagonist telmisartan was associated with significant dose - dependent reductions in systemic arterial blood pressure and pulmonary pressures . - pubmed
Although L - N ( G )- nitroarginine methylester ( L - NAME ) ( 100 microM , n=8 ) had no effect , superoxide dismutase ( 10 U ml(-1))+catalase ( 100 U ml(-1 ) ) increased the number of ventricular ectopic beats from 91+/-32 to 286+/-83 ( n=5 ) and augmented the incidence of irreversible ventricular fibrillation from 0 % to 60 % . - pubmed
The finding that some B and T cell epitopes were localized to regions with amino acid substitutions may account for the putative differential responsiveness to this antigen in tuberculosis and leprosy . - pubmed
A blood pressure decrease was observed with both L - arginine - rich diets ( Diet 2 v 1 , SBP : -6.2 mm Hg [ 95 % CI : -0.5 to -11.8 ] , DBP : -5.0 mm Hg [ -2.8 to -7.2 ] ; Diet 3 v 1 , SBP : -6.2 mm Hg [ -1.8 to -10.5 ] , DBP : -6.8 mm Hg [ -3.0 to -10.6 ] ) . - pubmed
Serum total cholesterol ( P = .06 ) and triglyceride ( P = .009 ) decreased and HDL cholesterol increased ( P = .04 ) after Diet 2 , but not after Diet 3 . - pubmed
The HDMF levels determined in the wines studied using a stable isotope dilution assay ( SIDA ) method were consistent with the results found by sensory analysis and GC / O ; i.e. - pubmed
Exposure of HBECs of asthmatic subjects to both 50 to 100 ppb O3 and 200 to 400 ppb NO2 significantly increased the release of IL-8 , GM - CSF , RANTES , and sICAM-1 from these cells after 24 hours of incubation . - pubmed
However , 50 to 100 ppb O3 and 200 to 400 ppb NO2 led to a significant increase in release of only IL-8 and sICAM-1 from HBECs of nonasthmatic subjects after 24 hours ' incubation . - pubmed
CONCLUSION : These results suggest that O3 and NO2 may modulate airway diseases , such as asthma , by increasing the release of inflammatory mediators from bronchial epithelial cells and that the cells of asthmatic subjects may be more susceptible to the adverse effects of these pollutants . - pubmed
In contrast , at 1 week after the STZ injection only plasma ET-1 and glucose levels were elevated , suggesting that the hyperglycaemia might trigger the excess production of ET-1 . - pubmed
Incubation with high glucose promoted the release of ET-1 from the isolated mesenteric arterial bed . - pubmed
PvuII and XbaI restriction fragment length polymorphisms ( RFLPs ) for the estrogen receptor gene ( ERG ) and its relation to bone mineral density ( BMD ) were examined in 454 postmenopausal Chinese women , aged 55 - 79 years . - pubmed
Two important factors have been taken into account in compiling these recommendations : ( 1 ) the lack of level I evidence to convincingly demonstrate the value of HER2 as a predictive marker for resistance or sensitivity to chemo- and hormonal therapy , and ( 2 ) the recently published pivotal phase II and III trial data proving the efficacy of trastuzumab as a single agent and in combination with chemotherapy in HER2 - positive metastatic breast cancer . - pubmed
Because nonselective cycloooxygenase ( COX ) inhibition attenuated anorexia after lipopolysaccharide ( LPS ) administration , we tested the ability of resveratrol ( 2.5 , 10 , and 40 mg / kg ) and NS-398 ( 2.5 , 10 , and 40 mg / kg ) , selective inhibitors of the two COX isoforms COX-1 and - 2 , respectively , to attenuate LPS ( 100 microg / kg ip)-induced anorexia . - pubmed
Because prostaglandin ( PG ) E ( 2 ) is considered the major metabolite synthesized by COX , we measured plasma and cerebrospinal fluid ( CSF ) PGE ( 2 ) levels after LPS administration . - pubmed
LPS induced a time - dependent increase of PGE ( 2 ) in CSF but not in plasma . - pubmed
NS-398 ( 5 , 10 , and 40 mg / kg ) blocked the LPS - induced increase in CSF PGE ( 2 ) , whereas resveratrol ( 10 mg / kg ) did not . - pubmed
These results support a role of COX-2 in mediating the anorectic response to peripheral LPS and point at PGE ( 2 ) as a potential neuromodulator involved in this response . - pubmed
The main results can be summarized as follows : ( 1 ) urinary excretion of adrenaline , noradrenaline and cortisol was facilitated , and the amounts of urinary excretion were significantly higher when TC were worn . - pubmed
Heart rate was significantly higher in the TC group ; ( 2 ) nocturnal urinary melatonin excretion was significantly greater in the TC group . - pubmed
Functional copper at the acetyl - CoA synthase active site . - pubmed
The bifunctional CO dehydrogenase / acetyl - CoA synthase ( CODH / ACS ) plays a central role in the Wood - Ljungdahl pathway of autotrophic CO ( 2 ) fixation . - pubmed
Depletion of copper correlates with reduction in ACS activity and in intensity of the " NiFeC " EPR signal without affecting either the activity or the EPR spectroscopic properties associated with CODH . - pubmed
In contrast , Zn content is negatively correlated with ACS activity without any apparent relationship to CODH activity . - pubmed
XAS studies are consistent with a distorted Cu ( I )- S ( 3 ) site in the fully active enzyme in solution . - pubmed
These results indicate an essential and functional role for copper in the CODH / ACS from acetogenic and methanogenic organisms . - pubmed
8024 - 37 - 1 ) ( Major Component 79%-85 % Curcumin , CAS No . - pubmed
THE EFFECT OF PROTEIN LEVEL AND AMINO ACID BALANCE UPON THYROID ACTIVITY IN THE CHICK . - pubmed
The cumulative incidence of severe ( grade III - IV ) acute GVHD and extensive chronic GVHD was 4 % , 0 % , 44 % and 11 % , 8 % , 44 % in groups receiving ATG in a dose of 8 mg / kg , 6 mg / kg , and 4 mg / kg , respectively ( severe acute GVHD : p<0.001 ; extensive chronic GVHD : p=0.05 ) . - pubmed
CONCLUSION : Based on these findings , we recommend when ATG is used in the setting of stem cell transplantation with ( partially ) TCD grafts from MUDs , to give a total dose of 6 to 8 mg / kg . - pubmed
Both the activation of DNases and chromatin fragmentation are suppressed in the presence of the caspase 3 / 7 inhibitor Ac - DEVD - CHO . - pubmed
Topoisomerase I - mediated DNA cleavage as a guide to the development of antitumor agents derived from the marine alkaloid lamellarin D : triester derivatives incorporating amino acid residues . - pubmed
The marine alkaloid lamellarin D ( LAM - D ) has been recently characterized as a potent poison of human topoisomerase I endowed with remarkable cytotoxic activities against tumor cells . - pubmed
Two groups of triester compounds incorporating various substituents on the three phenolic OH at positions 8 , 14 and 20 of 6H -[ 1 ] benzopyrano [ 4 ' , 3 ' : 4,5 ] pyrrolo [ 2,1 - a ] isoquinolin-6 - one pentacyclic planar chromophore typical of the parent alkaloid were tested as topoisomerase I inhibitors . - pubmed
The non - amino compounds in group A showed no activity against topoisomerase I and were essentially non cytotoxic . - pubmed
In sharp contrast , compounds in group B incorporating amino acid residues strongly promoted DNA cleavage by human topoisomerase I. - pubmed
LAM - D derivatives tri - substituted with leucine , valine , proline , phenylalanine or alanine residues , or a related amino side chain , stabilize topoisomerase I - DNA complexes . - pubmed
The DNA cleavage sites detected at T downward arrow G or C downward arrow G dinucleotides with these molecules were identical to that of LAM - D but slightly different from those seen with camptothecin which stimulates topoisomerase I - mediated cleavage at T downward arrow G only . - pubmed
In the DNA relaxation and cleavage assays , the corresponding Boc - protected compounds and the analogues of the non - planar LAM-501 derivative lacking the 5 - 6 double bond in the quinoline B - ring showed no effect on topoisomerase I and were considerably less cytotoxic than the corresponding cationic compounds in the LAM - D series . - pubmed
LAM - D is the lead compound of a new promising family of antitumor agents targeting topoisomerase I and the amino acid derivatives appear to be excellent candidates for a preclinical development . - pubmed
is likely but has to be confirmed ; ( ii ) in male infertility , COH with gonadotropins in conjunction with i.u.i . - pubmed
improves the pregnancy rate but stimulation with clomifene citrate appears to be less effective than gonadotropins administration . - pubmed
The Akt / PKB kinase is an important cell survival protein which is regulated by D3 - phosphoinositides . - pubmed
[ Wide - spectrum beta - lactamases : are they a serious problem today in Spain ? ] - pubmed
Exposure of cells to ALA or its reduced form dihydrolipoic acid ( DHLA ) for 24 h dose dependently increased caspase-3 - like activity and was associated with DNA - fragmentation . - pubmed
Increased mitochondrial O2 -*-production was preceded by an increased influx of lactate or pyruvate into mitochondria and resulted in the down - regulation of the anti - apoptotic protein bcl - X ( L ) . - pubmed
Moreover , when the lactate / pyruvate transporter was inhibited by 5 - nitro-2 -( 3 - phenylpropylamino ) benzoate , ALA- and DHLA - induced mitochondrial ROS - production and apoptosis were blocked . - pubmed
Variations in serum parameters like osteocalcin , c - telopeptide , levels of elements like Calcium , Magnesium , Phosphorus , concentration of vitamin - D metabolites , lactate levels , bicarbonate concentrations , amount of alkaline phosphatase are demonstrated in the course of development of osteoporosis . - pubmed
Mixed lysate assays demonstrate that this is the result of endogenous inhibitors present in early embryos , suggesting a model of matrix remodeling regulated by spatially heterogeneous MMP inhibition . - pubmed
Isomerization of L - aspartate and deamidation of L - asparagine in proteins or peptides dominantly give rise to L - isoaspartate by a non - enzymatic reaction via succinimide as a intermediate under physiological conditions . - pubmed
We described here that the formation of isoaspartate is enhanced in amyloid - beta ( Abeta ) peptides in Alzheimer ' s disease ( AD ) . - pubmed
Specific antibodies recognizing isoaspartate of Abeta revealed that isomerized Abeta peptides were deposited in senile plaques as well as amyloid - bearing vessels . - pubmed
Furthermore , systematic proline substitution analyses revealed that the beta - turn structure at positions 22 and 23 of Abeta42 plays a crucial role in the aggregation and neurotoxicity of Abeta peptides . - pubmed
Protein L - isoaspartyl methyltransferase ( PIMT ) is a putative protein repair enzyme , which converts L - isoaspartyl residues in damaged proteins to normal L - aspartyl residues . - pubmed
The one - pot conversion of ( + /-)- citronellal to menthol can be selectively catalysed by either a bifunctional Ni / Zr - zeolite beta catalyst or a dual catalyst system of Zr - beta and Ni / MCM-41 , giving a high diastereoselectivity to ( + /-)- menthol of 90 - 94 % . - pubmed
We introduced methionines one - at - a - time into the region 163 - 176 of the parathyroid hormone receptor , and find that crosslinking occurs to the side - chain of methionine over a range of 11 amino acids . - pubmed
The enzyme had a K(m ) of 190 micromolar for its substrate , 12 - oxo - phytodienoic acid . - pubmed
The preferred reductant was NADPH , for which the enzyme exhibited a K(m ) of 13 micromolar , compared with 4.2 millimolar for NADH . - pubmed
Recently , postsource decay ( PSD ) fragment spectra obtained by matrix - assisted laser desorption - ionization time - of - flight mass spectrometry ( MALDI - TOF MS ) have provided critical information on complex carbohydrates . - pubmed
On the basis of the MALDI - PSD spectra , we deduced fragmentation rules for phosphorylated N - glycans that will be valuable for distinguishing the position of phosphorylation . - pubmed
In the present study , this first - shot phenomenon has been investigated for the hexameric protein complex allophycocyanine ( APC ) by two independent methods , MALDI - MS with a ( nearly ) pH - neutral matrix 6 - aza-2 - thiothymine ( 6 - ATT ) and by imaging the fluorescence of the complex in APC-6 - ATT preparations by confocal laser scan microscopy ( CLSM ) . - pubmed
STUDY OBJECTIVE : To test the hypothesis that dexmedetomidine , a selective alpha-2 agonist , enhances urine flow rate and perioperative renal function , a post hoc analysis was conducted on a recently completed study of dexmedetomidine used as an adjunct to epidural analgesia after thoracotomy . - pubmed
Melatonin increases stress fibers and focal adhesions in MDCK cells : participation of Rho - associated kinase and protein kinase C. - pubmed
An optimal increase in water transport , concomitant with elevated stress fiber ( SF ) formation , occurs at nocturnal plasma melatonin concentrations ( 1 nm ) after 6 hr of incubation . - pubmed
Blockage in melatonin - elicited dome formation was observed with protein kinase C ( PKC ) inhibitors . - pubmed
Despite , this information on the precise mechanism by which melatonin increases SF formation involved in water transport is not known . - pubmed
SF organization and vinculin phosphorylation are involved in FAC assembly and both processes are mediated by PKC , an enzyme stimulated by melatonin ; in these processes also involved is Rho - associated kinase ( ROCK ) . - pubmed
Thus , we studied FAC formation and the ROCK / PKC pathway as the mechanism by which melatonin increases SF formation and water transport . - pubmed
The results showed that 1 nM melatonin and the PKC agonist phorbol-12 - miristate-13 - acetate increased FAC . - pubmed
The PKC inhibitor GF109203x , and the ROCK inhibitor Y27632 , blocked increased FAC caused by melatonin . - pubmed
ROCK and PKC activities , vinculin phosphorylation and FAC formation were increased with melatonin . - pubmed
The PKC inhibitor , GF109203x , abolished both melatonin stimulated FAC in whole cells and ROCK activity , indicating that ROCK is a downstream kinase in the melatonin - stimulated PKC pathway in MDCK cultured cells that causes an increase in SF and FAC formation . - pubmed
The intercalative interaction of the Cu ( II ) complexes with calf - thymus DNA ( CT - DNA ) was probed by UV / VIS and fluorescence titration , as well as by thermal - denaturation experiments , and the intrinsic binding constants ( K(b ) ) for the complexes with 1 and 2 were 4.2x10(3 ) and 4.9x10(3 ) M(-1 ) , resp . - pubmed
Hemoglobin vesicle ( HbV ) , which is also called liposome - encapsulated hemoglobin , functions as a hemoglobin - based oxygen carrier and is expected to be utilized in emergency situations including hemorrhagic shock and several kinds of ischemic diseases . - pubmed
Endovascular therapy was performed with coils in seven cases , n - butyl-2 - cyanoacrylate ( NBCA ) in two cases and with both in one case . - pubmed
We did not notice change in the levels of creatinine phosphokinase , serum creatinine or lover enzymes . - pubmed
Role of ceramide in membrane protein organization investigated by combined AFM and FCS . - pubmed
Our results show that components with poor affinity toward the liquid - ordered phase , such as several fluorescent lipid analogues or the synaptic protein Synaptobrevin 2 , are excluded from ceramide - rich domains . - pubmed
Conversely , we show for the first time that the raft - associated protein placental alkaline phosphatase ( GPI - PLAP ) and the ganglioside GM1 are enriched in such domains , while exhibiting a strong decrease in lateral diffusion . - pubmed
Labeled target DNA was prepared by polymerase chain reaction amplification of 16S rRNA genes using a Cy5 - labeled universal bacterial forward primer and a universal reverse primer . - pubmed
RESULTS : Physical activity , BMI , high - density lipoprotein cholesterol , smoking , depression , and hypertension were directly associated with CRP and IL-6 and inversely associated with one or more HRV variables . - pubmed
The 3 -( 4 - cyano-3 - trifluoromethylphenyl )- 5,5 - dimethylthiohydantoin derivatives which have carboxy - terminal side chains were synthesized and their agonistic / antagonistic activities against androgen receptor ( AR ) measured . - pubmed
Three ionic liquid ( IL)-based aggregates , 1 - hexadecyl-3 - methylimidazolium bromide ( HDMIm - Br ) , 1 - hexadecyl-3 - butylimidazolium bromide ( HDBIm - Br ) , and 1,3 - didodecylimidazolium bromide ( DDDDIm - Br ) have been applied to the development of a quantitative solid - phase microextraction ( SPME)-gas chromatography ( GC)-mass spectrometry ( MS ) method . - pubmed
A sensitivity factor ( SF ) is defined and introduced for the first time to quantitatively compare the efficiency of the IL - based aggregates by SPME - GC - MS and to evaluate the partitioning strength of several polycyclic aromatic hydrocarbons ( PAHs ) to the three IL - aggregates . - pubmed
The ILs HDBIm - Br and HDMIm - Br have been used successfully to extract seven PAHs from the certified reference sediment BCR-535 using focused microwave - assisted extraction followed by SPME - GC - MS . - pubmed
When the association of cyclin B1 with positive MIB1 is also considered , our results support the role of cell proliferation in FDG uptake by the tumour . - pubmed
Oleanolic acid is a selective farnesoid X receptor modulator . - pubmed
Bile acid biosynthesis and catabolism are in part controlled in the liver by transcription factor farnesoid X receptor ( FXR ) . - pubmed
OA also dose - dependently suppressed the activity of FXR - LBD induced by its endogenous ligand chenodoxycholic acid ( CDCA ) . - pubmed
Consistently , OA partially blocked the ability of CDCA to induce a FXR target gene bile salt export protein ( BSEP ) . - pubmed
An enzymatic method to distinguish tetrahydrobiopterin from oxidized biopterins using UDP - glucose : tetrahydrobiopterin glucosyltransferase . - pubmed
We developed an enzymatic method to distinguish BH4 from the oxidized forms by employing BH4 : UDP - glucose alpha - glucosyltransferase ( BGluT ) , which catalyzes glucosyl transfer from UDP - glucose to BH4 . - pubmed
The BGluT - catalyzed reaction not only would reduce the burden of chromatographic separation but also would promise non - HPLC analysis of BH4 . - pubmed
RESULTS : Serum free T4 and T3 levels , adjusted for levothyroxine dose per kilogram body weight , decreased by 11 and 18 % , respectively , whereas TSH levels increased . - pubmed
Studies have shown that cytosolic GS isoforms are involved in nitrogen remobilization during leaf senescence and emphasized a role in seed production particularly in small grain crop species . - pubmed
It is expected that understanding the regulation of GS under varied climatic conditions will emerge as an important new area considering the results from recent studies that have shown nitrogen assimilation to be critical to plant acclimation to high CO ( 2 ) concentrations . - pubmed
An EPR study on powdered samples confirms the presence of Mn ( II ) , Fe ( III ) and Cr ( III ) in the minerals . - pubmed
Role of STIM1 / Orai1 - mediated store - operated Ca2 + entry in airway smooth muscle cell proliferation . - pubmed
Store - operated Ca ( 2 + ) ( SOC ) entry ( SOCE ) occurs in response to the intracellular sarcoplasma reticulum ( SR)/endoplasmic reticulum ( ER ) Ca ( 2 + ) store depletion . - pubmed
Stromal interaction molecule ( STIM ) 1 has been proposed as an ER / SR Ca ( 2 + ) sensor and translocates to the ER underneath the plasma membrane upon depletion of the ER Ca ( 2 + ) store , where it interacts with Orai1 , the molecular component of SOC channels , and brings about SOCE . - pubmed
We found that SOCE was upregulated during ASMC proliferation accompanied by a mild increase of STIM1 and a significant increase of Orai1 mRNA expression , whereas the proliferation of ASMCs was partially inhibited by the SOC channel blockers SKF-96365 , NiCl ( 2 ) , and BTP-2 . - pubmed
Moreover , after knockdown of STIM1 or Orai1 , the SOC channel blocker SKF-96365 had no inhibitory effect on the proliferation of ASMCs anymore . - pubmed
The existence of a stable pi - electron complex ( called A1 ) between a tert - butyl cation and a benzene molecule has been confirmed . - pubmed
XPG is a structure - specific endonuclease required for nucleotide excision repair ( NER ) . - pubmed
The parameters for comparison included body mass index ( BMI ) , the distance from the renal hilum to scrotum ( DRS ) , semen quality and pH value , serum concentration of follicle - stimulating hormone ( FSH ) , luteinizing hormone ( LH ) , prolactin , testosterone , alkaline phosphatase ( Alk - p ) , lactate dehydrogenase ( LDH ) , testicular volume and discrepancy ( % ) , grade of varicocoele and peak retrograde flow ( PRF ) and spontaneous venous reflux ( SVR ) by colour Doppler ultrasound and scrotal temperature ( ST ) . - pubmed
A2 is an anaerobic gram - negative sulfate - reducing bacterium with remarkable tolerance to copper . - pubmed
The DB extract effectively reversed the HFD - induced elevations in plasma glucose and insulin levels , and the homeostasis model assessment for insulin resistance and oral glucose tolerance test values . - pubmed
These studies indicate that MLN0905 modulates the pharmacodynamic biomarker phosphorylated histone H3 ( pHisH3 ) in tumor tissue . - pubmed
If ovulation can not be induced by CC , then either laparoscopic ovarian drilling ( LOD ) or gonadotropin therapy is selected as a subsequent treatment . - pubmed
IUI with administration of CC plus recombinant follicle - stimulating hormone ( rFSH ) was carried out ; however , pregnancy was not achieved . - pubmed
In the first attempt , the development of several follicles was observed under the gonadotropin releasing hormone ( GnRH ) agonist long protocol . - pubmed
Similarly , CAB - E4 was also found to decrease the production of EPS ( 43 - 57 % ) , hemolysin ( 43 - 57 % ) and biofilms ( 80 - 85 % ) of test pathogens . - pubmed
A drug for the treatment of sexual dysfunction , sildenafil , enhances the electrically evoked release of OT from the posterior pituitary . - pubmed
Copeptin as a marker of relative arginine vasopressin deficiency after pediatric cardiac surgery . - pubmed
The breakdown of L - arginine to ornithine and urea by host arginase supports Leishmania proliferation in macrophages . - pubmed
OBJECTIVE : To evaluate the difference in time until medical clearance when comparing tincture of opium ( TO ) to oral morphine ( OM ) in the treatment of neonatal abstinence syndrome ( NAS ) . - pubmed
MAIN OUTCOME MEASURE(S ): The primary outcome is to compare the time it takes for a baby being treated with TO versus OM for NAS to be medically cleared for discharge . - pubmed
RESULTS : The median time until medical clearance for those treated with TO was 29.5 days compared to 37 days for those treated with OM ( p = 0.14 ) . - pubmed
CONCLUSION : There was no statistically significant difference in the time it takes for a baby being treated with TO versus OM for NAS to be medically cleared for discharge . - pubmed
There are a number of safety benefits in using OM compared to TO . - pubmed
PURPOSE : The purpose of this study was to investigate the possible association between single nucleotide polymorphisms ( SNPs ) rs1800629 ( TNF - alpha -308 ) and rs361525 ( TNF - alpha -238 ) of the tumour necrosis factor ( TNF)-alpha gene and susceptibility to osteoarthritis ( OA ) in the Han Chinese population . - pubmed
After the separation of proteins under these first metal - free conditions , the metal ions in the gel fractions are eluted , followed by derivatization of copper ions into the metal probe complexes to be separated and determined by fluorescence detection in the second PAGE . - pubmed
In this PAGE - based method , the copper ions bound to ceruloplasmin and superoxide dismutase were quantitatively determined , in addition to the exchangeable albumin - bound copper ions . - pubmed
INTERVENTIONS : ISI was estimated from serial measurements of plasma insulin and glucose during an iv glucose tolerance test . - pubmed
Polo - like kinase 2 ( PLK2 ) was previously proposed as an important kinase in alpha - synuclein phosphorylation at Ser129 . - pubmed
Whereas PLK2 knockdown had no effect on Total - alpha - synuclein brain levels , it resulted in a gene - dosage dependent , albeit incomplete , reduction of endogenous Phospho - alpha - Syn levels in all brain regions investigated . - pubmed
These results support a significant role for a PLK kinase in phosphorylating alpha - synuclein at Ser129 in the brain , and suggest that PLK2 is responsible for this activity under physiological conditions . - pubmed
Dutasteride treatment contributed to a significant increase in BMD in group A. - pubmed
Asp(112 ) , in the middle of the S1 transmembrane helix , is an essential part of the selectivity filter in wild - type ( WT ) channels . - pubmed
After neutralizing Asp(112 ) by mutating it to Ala ( D112A ) , we introduced Asp at each position along S1 from 108 to 118 , searching for " second site suppressor " activity . - pubmed
The S4 segment of this mutant has similar accessibility to WT in open channels , because R211H / D112V / V116D was inhibited by internally applied Zn ( 2 + ) . - pubmed
At both positions where it produces proton selectivity , Asp frequently engages in salt linkage with one or more Arg residues from S4 . - pubmed
The type 4 P - type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry . - pubmed
Here we show that the carboxyl - terminal cytoplasmic region of Drs2p directly binds to Rcy1p , an F - box protein that is also required for endocytic recycling . - pubmed
The Drs2p - binding region was mapped to the amino acids 574 - 778 region of Rcy1p and a mutant Rcy1p lacking this region was defective in endocytic recycling of a v - SNARE Snc1p . - pubmed
In this work , we propose a novel glucose binding mechanism on a highly sensitive SERS substrate , in order to overcome challenges in specific glucose detection in bio - fluids . - pubmed
Chloroethene degradation and expression of Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments . - pubmed
Computational & amp ; experimental evaluation of the structure / activity relationship of beta - carbolines as DYRK1A inhibitors . - pubmed
AOM was first found to be coupled with sulfate reduction and mediated by anaerobic methanotrophic archaea ( ANME ) and sulfate - reducing bacteria ( SRB ) . - pubmed
Moreover , manganese ( Mn ( 4 + ) ) and iron ( Fe ( 3 + ) ) can also be used as electron acceptors of AOM . - pubmed
Analogous to a eukaryotic cap , 5'-NAD modification is shown in vitro to stabilize RNA against 5'-processing by the RNA - pyrophosphohydrolase RppH and against endonucleolytic cleavage by ribonuclease ( RNase ) E. - pubmed
The nudix phosphohydrolase NudC decaps NAD - RNA and thereby triggers RNase - E - mediated RNA decay , while being inactive against triphosphate - RNA . - pubmed
In vivo , ~13 % of the abundant sRNA RNAI is NAD - capped in the presence , and ~26 % in the absence , of functional NudC. - pubmed
Ethanol and corticotropin releasing factor receptor modulation of central amygdala neurocircuitry : An update and future directions . - pubmed
Much of the work examining the mechanisms by which the central amygdala mediates the development of alcohol dependence has focused on the interaction of acute and chronic ethanol with central amygdala corticotropin releasing factor signaling . - pubmed
Much of this work has primarily focused on the hypothesis that ethanol utilizes endogenous corticotropin releasing factor signaling to upregulate inhibitory GABAergic transmission in the central amygdala . - pubmed
Work that is more recent suggests that corticotropin releasing factor also plays an important role in mediating anxiety - like behaviors via the enhancement of central amygdala glutamatergic transmission , implying that ethanol / corticotropin releasing factor interactions may modulate excitatory neurotransmission in this brain region . - pubmed
The micelle would be successful in increasing drug solubility , however it rather decreased permeability of model drug progesterone ( Biopharmaceutics Classfication System ( BCS ) Class II ) as an overall flux . - pubmed
Thirdly , in the case of injection of paclitaxel ( BCS Class II)formulation , the drug was adsorbed to albumin . - pubmed
However , it was observed that some possible n - butanol tolerance mechanisms did not occurred upon the ATF expression , especially the membrane - related functions such as the homeoviscous adaptation , iron uptaking system , and efflux pump system . - pubmed
The purpose of this study was to establish a population pharmacokinetic ( PK ) model of tacrolimus and evaluate the influence of clinical covariates , including the genetic polymorphisms of the cytochrome P450 3A5 gene ( CYP3A5 ) and gene - encoding P - glycoprotein ( ABCB1 ) , on the PK parameters in Chinese adult liver transplant recipients . - pubmed
In the final model , CYP3A5 genotype , post - operative day , alanine aminotransferase , total bilirubin , hematocrit and blood urea nitrogen were found to significantly influence the CL / F , whereas POD and HB influence V / F. 4 . - pubmed
Population PK analysis of tacrolimus in Chinese adult liver transplant patients resulted in identification of the CYP3A5 genotype , POD , BUN , ALP , HCT , TBIL and HB as significant covariates on the PK parameters of tacrolimus . - pubmed
MATERIALS AND METHODS : The number of primary AECIIs , their viability , and cell cycle and apoptosis were studied under KGF treatment using a hemocytometer , trypan blue exclusion , and flow cytometry , respectively . - pubmed
The advantage of this design is that the targeted knockin retains the intact endogenous aggrecan locus and expresses the tamoxifen - inducible CreERT2 protein from a second IRES - driven open reading frame . - pubmed
Hereditary 1 , 25 - dihydroxyvitamin D - resistant rickets ( HVDRR ) , a rare recessive disease , is caused by mutation in the VDR gene encoding the vitamin D receptor leading to the resistance to vitamin D. - pubmed
Insulin Resistance in Nondiabetic Peritoneal Dialysis Patients : Associations with Body Composition , Peritoneal Transport , and Peritoneal Glucose Absorption . - pubmed
Leptin , adiponectin , insulin - like growth factor - binding protein 1 ( IGFBP-1 ) , and daily glucose absorption were also measured . - pubmed
FM ( 7.3 [ 12.3 , interquartile range ] versus 0.7 [ 1.4 , interquartile range ] , P<0.001 , and 39.4 + - 10.1 % versus 27.2 + - 11.5 % , P=0.002 , respectively ) , lower IGFBP-1 ( 8.2 + - 7.2 versus 21.0 + - 16.3 ng / ml , P=0.002 ) , but similar glucose absorption and small - solute transport compared with patients without insulin resistance . - pubmed
Urinary and serum levels of NGAL have been investigated as a new biomarker of acute kidney injury ( AKI ) , for an earlier and more accurate detection method than with creatinine level . - pubmed
Moreover , we purified the NGAL with Ni - Sepharose column . - pubmed
The dynamics and the vibrational signatures are used to interpret IR - MPD spectra recorded in the NH / OH stretch region . - pubmed
Indole based tubulin polymerization inhibitors : An update on recent developments . - pubmed
Diverse classes of naturally occurring as well as synthetic and semi - synthetic compounds with an indole nucleus induce microtubule polymerization and depolymerization and thereby change tubulin dynamics . - pubmed
The report mainly focuses on the brief synthetic methodology with the relevant SAR studies of different indole derived molecules that have been reported in the past few years as potential inhibitors of tubulin polymerization is discussed . - pubmed
The cellular and molecular effects of oxysterols were then evaluated on primary cultures of ASCs focusing on cell viability , adipogenic differentiation , and " canonical " WNT and MAPK signaling pathways . - pubmed
Notably , the accumulation of oxysterols in adipocytes was predicted by the adipose cell size of the donor ( R2 = 0.582 ; P & lt ; .01 ) . - pubmed
Challenging ASCs with free radical - derived type I ( 7kappa - C ) and type II ( 5,6 - Secosterol ) oxysterols led to a time- and concentration - dependent decrease of cell viability . - pubmed
We found a statistically significant increase ( nominal p & lt ; 0.05 ) in the expression of UGT1A1 , UGT1A3 , UGT1A4 , UGT1A5 , UGT1A6 , UGT2B7 , and UGT2B17 , as well as glucuronidation activities of serotonin , testosterone , and vorinostat during the first 25 years of life . - pubmed
Greater RPE was reported in 4vs4 - NO ( p & lt ; 0.01 ) and 4vs4 + 2IW - NO ( p & lt ; 0.01 ) compared with 4vs4 + 2IW+2IEW - NO , and during 4vs4 - GK ( p & lt ; 0.01 ) than 4vs4 + 2IW+2IEW - GK . - pubmed
Pterostilbene attenuates high glucose - induced oxidative injury in hippocampal neuronal cells by activating nuclear factor erythroid 2 - related factor 2 . - pubmed
High glucose medium ( 100mM ) decreased cellular viability after 24h incubation which was evidenced by : ( i ) reduced mitochondrial complex I and III activities ; ( ii ) reduced mitochondrial cytochrome C ; ( iii ) increased reactive oxygen species ( ROS ) generation ; ( iv ) decreased mitochondrial membrane potential ( DeltaPsim ) ; and ( v ) increased lactate dehydrogenase ( LDH ) levels . - pubmed
PTE ( 2.5 , 5 , and 10muM for 24h ) was nontoxic and induced the nuclear transition of Nrf2 . - pubmed
Pretreatment of PTE ( 2.5 , 5 , and 10muM for 2h ) displayed a dose - dependently neuroprotective effect , as indicated by significantly prevented high glucose - induced loss of cellular viability , generation of ROS , reduced mitochondrial complex I and III activities , reduced mitochondrial cytochrome C , decreased DeltaPsim , and increased LDH levels . - pubmed
In addition , the elevation of nuclear Nrf2 by PTE treatment ( 10muM for 2h ) was abolished by Nrf2 siRNA . - pubmed
Overall , the present findings were the first to show that pterostilbene attenuated high glucose - induced central nervous system injury in vitro through the activation of Nrf2 signaling , displaying protective effects against mitochondrial dysfunction - derived oxidative stress . - pubmed
The plant - specific PIN - FORMED ( PIN ) auxin efflux proteins have been well characterized in many plant species , where they are crucial in the regulation of auxin transport in various aspects of plant development . - pubmed
NtPIN4 expression was strongly induced by the application of exogenous indole-3 - acetic acid ( IAA ) , but was downregulated by the application of ABA , a strigolactone analogue , and cytokinin , as well as by decapitation treatments , suggesting that the NtPIN4 expression level is likely positively regulated by auxin . - pubmed
Therefore , NtPIN4 offers an opportunity for studying auxin - dependent branching processes . - pubmed
Resveratrol protects bupivacaine - induced neuro - apoptosis in dorsal root ganglion neurons via activation on tropomyosin receptor kinase A. - pubmed
QRT - PCR and western blot assays were also conducted to evaluate gene and protein expressions of tropomyosin receptor kinase A / B / C ( TrkA / B / C ) and activated ( phosphorylated ) Trk receptors , phospho - TrkA / B / C. - pubmed
qRT - PCR and western blot showed that RSV did not alter TrkA / B / C gene or protein expression , but significantly upregulated phospho - TrkA. - pubmed
CONCLUSION : Resveratrol may protect anesthesia - induced DRG neuro - apoptosis , and activation of TrkA signaling pathway may be the underlying mechanism in this process . - pubmed
Acrylamide and N , N ' - methylene bisacrylamide was used as carrier materials and the N - terminal epitope ofnicotinic acetylcholine receptor alpha7 ( nAchR alpha7 ) was tested as a template molecule , and the polymer nanoparticleswere obtained after polymerization and template removal . - pubmed
Specifically , P7C3 promoted the expression of neurogenesis - associated proteins , including doublecortin , beta tubulin III ( beta - tub3 ) , adam11 and adamts20 , near the peri - infarct cortex , accompanied by glycogen synthase kinase 3 ( GSK-3 ) inhibition and beta - catenin upregulation . - pubmed
The application of a specific inhibitor against glucagon - like peptide 1 receptor ( GLP-1R ) , exendin ( 9 - 39 ) , revealed that the beneficial effects of P7C3 involved triggering the activation of GLP-1R - associated PKA / Akt signaling . - pubmed
P7C3 elicited the GLP-1R - dependent intracellular cAMP increment and the insulin secretion in cellular models . - pubmed
Our findings indicate that P7C3 promotes the expression of neurogenesis proteins by activation of the cAMP / PKA - dependent and Akt / GSK3 - associated beta - catenin through positive allosteric stimulation of GLP-1R. - pubmed
UV - vis of Cu - NPLs showed an SPR characteristic peak at 563 nm ( energy bandgap = 2.1 eV ) . - pubmed
Morphology and size of the as - prepared Cu - NPLs were determined using SEM and TEM studies . - pubmed
TEM observations show that the produced Cu - NPLs are hemispherical in shape with different diameters in the range 4.7 - 17.4 nm . - pubmed
Cell proliferation in FXR1 - deficient cells was evaluated by cell counting and MTT assays . - pubmed
The ESR1 open reading frame ( ORF ) was 1 , 767 bp encoding 588 amino acids . - pubmed
For BMPR1B , the ORF was 1599 bp encoding 532 amino acids , and an ORF of 906 bp encoding 301 amino acids was identified in FOXL2 . - pubmed
Methods Nine fentanyl analogues ( 2 , 2 '- difluorofentanyl , acetyl fentanyl , fentanyl , butyl fentanyl , valeryl fentanyl , acryloyl fentanyl , furan fentanyl , 4 - fluorine isobutyl fentanyl , carfentanyl ) were selected and analyzed with ultra - high performance liquid chromatography - quadrupole time - of - flight - mass spectrometry ( UHPLC - QTOF - MS ) and gas chromatography - mass spectrometry ( GC - MS ) . - pubmed
Hence , we attempted to carry out in silico screening of benzene sulfonamide substituted pyrazole - pyrazoline series against Histo aspartic protease . - pubmed
Molecular dynamic simulation study of free enzyme and the enzyme complex with 4 -( 5 -( 4 - methoxyphenyl )- 1'phenyl-3 '-( p - tolyl )- 3,4 - 1'H,2H -[ 3,4 '- bipyrazol ]- 2 - yl ) benezenesulfonamide indicated structural stability . - pubmed
Tandem RNA isolation reveals functional rearrangement of RNA - binding proteins on CDKN1B / p27Kip1 3'UTRs in cisplatin treated cells . - pubmed
Here we introduce a novel biochemical approach , termed tobramycin - based tandem RNA isolation procedure ( tobTRIP ) , to quantify proteins associated with the 3'UTRs of cyclin - dependent kinase inhibitor 1B ( CDKN1B / p27Kip1 ) mRNAs in vivo . - pubmed
P27Kip1 plays an important role in mediating a cell 's response to cisplatin ( CP ) , a widely used chemotherapeutic cancer drug that induces DNA damage and cell cycle arrest . - pubmed
RESULTS : A high expression of PRDX-1 was observed in 20 % of cases , and was associated with worse compliance to chemotherapy protocol ( P<0.002 ) , worse response to chemotherapy ( P<0.04 ) , and higher levels of CA 125 after the 1 < sup > st line treatment ( P<0.004 ) . - pubmed
This study explores the simultaneous application of fly ash ( FA ) generated from the thermal treatment of municipal solid waste as a CO2 sequester through aqueous mineral carbonation and as a supplementary cementitious material ( SCM ) for the development of green construction materials . - pubmed
Stratifying analyses by the levels of measurement of LAN exposures ( macro- , meso- and micro - levels ) and time of LAN measurement ( including before and while sleeping ) consistently produced robust estimates , with higher exposure to LAN being positively associated with poorer weight outcomes . - pubmed
Luteolin affects keloid fibroblast proliferation and apoptosis by regulating FRAT1 gene expression . - pubmed
The current experiment was performed to investigate whether luteolin affects the proliferation and apoptosis of keloid fibroblasts by regulating the expression of FRAT1 gene . - pubmed
MTT method , western blot , flow cytometry , and real - time quantitative PCR ( qPCR ) were used to detect cell proliferation , cyclin D1 ( CyclinD1 ) , p21 , B - cell lymphoma / leukemia-2 ( Bcl-2 ) , Bcl-2 related X protein ( Bax ) , FRAT1 protein expression , apoptosis and ARHI mRNA expression . - pubmed
Keloid fibroblasts were transfected with si - FRAT1 , or pcDNA - FRAT1 and treated with luteolin to observe their roles in cell proliferation and apoptosis . - pubmed
Compared with the control group , luteolin significantly reduced the keloid fibroblast activity , CyclinD1 , Bcl-2 , and FRAT1 protein levels , and obviously improved the cell apoptosis rate , p21 and Bax protein expression ( P<0.05 ) . - pubmed
FRAT1 overexpression reversed the inhibitory effect of luteolin on keloid fibroblast activity , FRAT1 , CyclinD1 , and Bcl-2 protein expression , and promotion of apoptosis , p21 and Bax protein expression . - pubmed
Luteolin can inhibit the proliferation and induce apoptosis of keloid fibroblasts by regulating the expression of FRAT1 gene . - pubmed
: Bar charts ( top ) show the percentage of BrdU ( a ) and Ki67 ( b ) positive cells relative to the percentage of DAPI stained nuclei ( y - axis ) in cells at baseline and serially passaged cells ( x - axis ) . - pubmed
: The bar charts ( right ) show the percentage of MAP-2 ( a ) , CC3 ( b ) , S100beta ( c ) and doublecortin ( DCX ) ( d ) -positive cells relative to the percentage of DAPI stained nuclei in cells at baseline and serially passaged cells . - pubmed
The mean value of diastolic and systolic blood pressures , pulse rate , and mean arterial pressure dioxide were significantly decreased in both PF and PP groups ( P Time < 0.001 ) , although there was no significant difference between groups . - pubmed
Cynaropicrin Shows Antitumor Progression Potential in Colorectal Cancer Through Mediation of the LIFR / STATs Axis . - pubmed
Western blotting results suggested that the antitumor effects of cynaropicrin might be mediated by inhibition of the LIFR / STATs axis . - pubmed
Cynaropicrin reduced the formation of STAT3 / STAT4 heterodimers and blocked their entry into the nucleus . - pubmed
Exposure to NPS ( 1 - 100 mug / L ) increased the glb-10 expression , and the glb-10(RNAi ) worm was susceptible to NPS toxicity in inducing reactive oxygen species ( ROS ) production and in decreasing locomotion behavior . - pubmed
Moreover , a signaling cascade of HRG-7 - HRG-5 required for the control of heme homeostasis was identified to function downstream of neuronal GLB-10 to regulate the NPS toxicity . - pubmed
Our results highlight the crucial function of heme homeostasis related signaling in regulating the NPS toxicity in organisms . - pubmed
The accumulation of dopa and 5 - hydroxytryptophan after inhibition of the aromatic amino acid decarboxylase with 3 - hydroxybenzylhyrazine HCl , served as a measure of catecholamine and 5 - hydroxytryptamine synthesis in vivo . - pubmed
The data are compatible with the view that dibutyryl cAMP stimulates tyrosine and tryptophan hydroxylase directly . - pubmed
The reaction of tris ( hydroxymethyl ) aminomethane with calf intestinal alkaline phosphatase . - pubmed
The effect of tris ( hydroxymethyl ) aminomethane concentrations on the rate of calf intestinal alkaline phosphatase - catalyzed hydrolysis of p - nitrophenyl phosphate was studied , in the pH range 8 - 10 , where no transphosphorylation reaction could be detected . - pubmed
Kinetic analysis of the results permitted description of the effect of Tris concentrations T on the rate of enzyme catalyzed hydrolysis ( V ) by the following equation : ( see article ) . - pubmed
The rate - accelerating effect of Tris concentrations can be ascribed to two different mechanisms : At moderate Tris concentrations ( 0.01 - 0.20 M ) the enzyme forms a reversible addition complex with a Tris molecule . - pubmed
We suggest that the binding of Tris to the enzyme could potentiate a second active site of the enzyme , due to its ionization effect upon an acidic group of the enzyme of pK = 8.9 . - pubmed
The modest linear rate accelerating effect of Tris at high concentrations ( 0.20 - 0.60 M ) could be ascribed to the change of the dielectric constant of the medium , the degree of solvation of the protein , or change in the tertiary structure of the enzyme . - pubmed
The mechanism of antisecretory activity currently remains unknown as UP 5145 - 52 fails to bind to receptors involved in gastric secretion ( H2 or muscarinic ) and to inhibit H +/ K + adenosine triphosphatase . - pubmed
The reaction required an NADPH - generating system and molecular oxygen and was inhibited by carbon monoxide , suggesting that a cytochrome P450 - linked mono - oxygenase system is prerequisite for the deactivation reaction . - pubmed
Intraperitoneal IL-2 and LAK cells led to elevations in the concentrations of SP and CGRP to respective maximal means of 319 and 175 pM after 8 hr , which were maintained for 24 - 48 hr without alterations in the levels of vasoactive intestinal peptide or somatostatin . - pubmed
Circadian rhythm of atrial natriuretic peptide , plasmatic renin activity , and plasma aldosterone in compensated cirrhosis of the liver . - pubmed
The activities of rat brain protein kinase C isoenzymic fractions separated by hydroxyapatite chromatography were measured with histone H1 or the oligopeptide Ala - Ala - Ala - Ser - Phe - Lys - Ala - Lys - Lys - amide as substrates . - pubmed
In some brain extracts a phospholipid - dependent but Ca2 + -inhibited protein kinase was also observed which was eluted from the hydroxyapatite column between type II and III isoenzymes of protein kinase C. - pubmed
Amino acids and peptides XII : synthetic peptides related to the N - terminal portion of fibrin alpha - chain and their inhibitory effects on fibrinogen / thrombin clotting . - pubmed
Of the seven sites at which nucleotide substitutions were confirmed using RNA templates , four resulted in amino acid changes in three different proteins , the leader peptide , 1B ( VP2 ) , and 1D ( VP1 ) . - pubmed
SOD derivatized with polyethylene glycol , which increases circulating half - life , was more effective , increasing survival to 80 % . - pubmed
Desferal - Fe3 + , which lacks in vitro SOD activity , or Mn2 + alone did not affect the survival of skin flaps , but Desferal alone was nearly as effective as the Desferal - Mn2 + mixture . - pubmed
The cleansing and application of a molluscicide ( copper sulphate ) to the raceways in spring resulted in a 56 % reduction in the numbers of metacercariae infecting trout during the following summer . - pubmed
The subunit structure has been analysed by SDS polyacrylamide gel electrophoresis of the highly purified preparation and by immunoprecipitation with a monoclonal antibody directed to the DNA polymerase . - pubmed
The present results indicate that an inhibition of beta 2 - mediated hepatic glycogenolysis by propranolol may also influence blood glucose homeostasis during exercise . - pubmed
Naloxone has been used as a pharmacological tool to investigate the role of endorphins and opiate receptors in the cardiovascular pathophysiology of shock . - pubmed
It would appear that endorphins act on opiate receptors to contribute to the abnormalities found and that naloxone improves survival as well as cardiovascular function in shock . - pubmed
Naloxone has served us well as a key to unlock the involvement of endorphins and opiate receptors in shock . - pubmed
Naloxone 's disadvantage of increasing pain awareness may limit its clinical usefulness but might be overcome by using drugs that reverse the behavioral and neuroendocrine changes produced by beta - endorphin without altering pain relief . - pubmed
Thyrotropin - releasing hormone ( TRH ) is just such a " physiological " opiate antagonist which has been shown to increase MAP in experimental endotoxic and hemorrhagic shock [ 32 ] . - pubmed
Calcium restriction and parathyroid hormone enhance renal compensatory growth . - pubmed
The data support the hypothesis that the inhibition of glucagon release from the A2 - cell is regulated via an intracellular , energy - dependent mechanism . - pubmed
Refolding of reduced and denatured hTSHR - ecd occurred in polyacrylamide gels treated with 0.25 M KCl for visualization of protein bands . - pubmed
Under conditions of partial renaturation , at least three forms of the hTSHR - ecd were resolved by reelectrophoresis using sodium dodecyl sulfate - polyacrylamide gel electrophoresis : 1 ) unfolded monomers , 2 ) folded monomers , and 3 ) tetramers . - pubmed
A natural variant of the hTSHR ( v1.3 ) , sharing 231 N - terminal amino acids with hTSHR - ecd , formed folded monomers and dimers on renaturation , but not tetramers , implicating one or more of the five cysteine residues residing between positions 231 - 415 in the association of dimers into tetramers . - pubmed
Na + , K (+)- ATPase ( the sodium pump ) is a ubiquitous enzyme that consumes ATP to maintain an adequate neuronal transmembrane electrical potential necessary for brain function and to dissipate ionic transients . - pubmed
To assess whether abnormalities in the sodium pump occur in this disease , we used [ 3H]ouabain to determine the density of Na + , K (+)- ATPase for each anatomic region of hippocampus by in vitro autoradiography . - pubmed
We estimated regional differences in capacity for ATP generation by determining cytochrome c oxidase ( CO ) activity . - pubmed
Both epilepsy groups exhibited slight but significant increases in sodium pump density / unit mass of protein in the dentate molecular layer , CA2 , and subiculum as compared with autopsy controls . - pubmed
Results suggest that although sodium pump protein in surviving neurons appears to be upregulated in epilepsy , sodium pump capacity may be limited by the reduced levels of CO activity . - pubmed
By far the most abundant message was that for amphiglycan , CD44 message was next in relative abundance , and the messages for fibroglycan , glypican and betaglycan were all expressed at low levels . - pubmed
Syndecan message could not be detected by this technique . - pubmed
In contrast with the fibroblasts , where the amphiglycan message exhibits no size polymorphism , the chondrocyte amphiglycan message is present in three polymorphic forms , due to the use of alternative polyadenylation signals . - pubmed
When the newly isolated chondrocytes are maintained in monolayer culture for several passages , the amphiglycan message heterogeneity reverts to that characteristic of the fibroblasts . - pubmed
Dichain - TeTx , with its light chain linked to the heavy chain by means of a disulfide bond , causes the block of exocytosis to develop more slowly than does the purified light chain . - pubmed
The disulfide bonds , which in both toxins hold the subunits together , were cleaved by the intrinsic thioredoxin - reductase system . - pubmed
Previous studies have shown that this plasmid , designated as pSMB 74 , encodes genes associated with the production of prepediocin , its post - translation processing to pediocin AcH , transmembrane translocation of these molecules , and immunity of producer cells against pediocin AcH. - pubmed
The four genes , each with independent ribosome binding sites ( rbs ) , initiation and termination codons and spacer sequences in between , were designated as pap A , pap B , pap C and pap D and encode respectively for proteins of 62 , 112 , 174 and 724 amino acids . - pubmed
Ca2 + efflux from cultured vascular smooth muscle cells ( VSMC ) consists of two mechanisms : one is dependent on extracellular sodium , mediated by the Na(+)-Ca2 + exchange ( Na+o - dependent Ca2 + efflux ) , and the other is independent of extracellular sodium but is mediated by the Ca ( 2 +)- pump ( Na+o - independent Ca2 + efflux ) . - pubmed
An increase in intracellular [ Ca2+]i may be compensated for by enhanced Ca ( 2 +)- pump and Na(+)-Ca2 + exchange activities in VSMC in hypertension . - pubmed
These responses can also be observed after exposure to forskolin , which activates cyclic AMP - dependent protein kinase A or after treatment with known activators of protein kinase C ( PKC ) such as phorbol-12 - myristate 13 - acetate ( PMA ) . - pubmed
The responses elicited by exposure to 8 - OH - DPAT or PMA can be blocked completely by inhibitors of PKC and also by 24 - hr exposure to PMA . - pubmed
Inhibition of phospholipase A2 with quinacrine or by removal of extracellular Ca ++ blocks the agonist - mediated loss of 5 - HT1A receptor binding sites . - pubmed
The carbohydrates recognized by mAbs 2B8 and 9 - OE were also abundantly expressed in the ducts and acini of the lingual salivary glands . - pubmed
These cells were normal in other parameters and could fire throughout a depolarizing current injection in the presence of inorganic calcium channel blockers or norepinephrine ( NE ) , suggesting that the inability to fire was not due to injury . - pubmed
NE decreased the slow Ca - dependent AHP via beta - adrenergic receptors . - pubmed
Serum - induced membrane depolarization in quiescent fibroblasts : activation of a chloride conductance through the G protein - coupled LPA receptor . - pubmed
We find that this serum activity is attributable to albumin - bound lysophosphatidic acid ( LPA ) acting on its own G protein - coupled receptor , and that membrane depolarization is due to activation of an anion conductance mediating Cl- efflux . - pubmed
This depolarizing Cl- current can also be activated by thrombin and neuropeptide receptors ; it is distinct from volume - regulated Cl- currents . - pubmed
Activation of the Cl- current consistently follows stimulation of phospholipase C and coincides with remodelling of the actin cytoskeleton , which is regulated by the Ras - related GTPase Rho . - pubmed
The results indicate that in quiescent fibroblasts , LPA and other G protein - coupled receptor agonists evoke membrane depolarization by activating a new type of Cl - channel through a signalling pathway that is closely associated with phosphoinositide hydrolysis , yet independent of known second messengers . - pubmed
Mean 24 h ambulatory SBP / DBP decreases were 6.6 + /- 18.0/8.4 + /- 8.5 mm Hg in the trandolapril group ( P & lt ; 0.001 ) and 5.7 + /- 11.1/7.2 + /-9.6 mm Hg in the nitrendipine group ( P & lt ; 0.001 ) . - pubmed
Mutation to Phe of all four cytoplasmic Tyr residues together ( ALL F mutant ) greatly reduced the ability of mCD28 to augment IL-2 production . - pubmed
Isolated re - constitution of Tyr 188 , but not 170 , 185 , or 197 , restored the ability of ALL F mCD28 to deliver a costimulus . - pubmed
The transposon Tn10 - encoded metal - tetracycline / H + antiporter ( TetA(B ) ) was preferentially photolabeled when [ 3H ] tetracycline was irradiated in the presence of energized inverted membrane vesicles containing the TetA protein . - pubmed
Photolabeling was not observed in vesicles containing the Asp-285 --> ; Asn mutant TetA protein , indicating that Asp-285 participates in the substrate binding or the step(s ) prior to substrate binding . - pubmed
After hospitalization , urinary C - peptide excretion ( UCP ) and 24 - hour serum profiles for glucose were measured before and after administration of NPH insulin 0.1 , 0.2 , and 0.3 U x kg body weight per day subcutaneously in a single dose on 4 consecutive days . - pubmed
To our knowledge no transcription factors have previously been shown to depend on testosterone or LH for expression in Sertoli cells in vivo . - pubmed
Collectively , the data implicate Pem as a candidate to regulate a subset of androgen - dependent genes in the male reproductive system . - pubmed
Concordance of nucleotide sequences from different tissue compartments obtained concurrently from individual patients was high : 98 % in the protease and 94 % in the reverse transcriptase regions . - pubmed
The prevalence of minimal or mild endometriosis was higher in women age 25 years or older , in those who reported menarche at the age of 13 years [ prevalence odds ratio ( POR ) = 1.63 ; 95 % confidence interval ( CI ) = 1.02 - 2.60 ] or older ( POR = 1.73 ; 95 % CI = 1.07 - 2.78 ) , menstrual cycles of 27 days or less ( POR = 1.63 ; 95 % CI = 1.02 - 2.60 ) , or caffeine intake of 300 mg per day or more ( POR = 1.33 ; 95 % CI = 0.91 - 1.94 ) . - pubmed
In the search for an alternative to chemical halogenation reactions using the free halogens , a novel type of halogenating enzymes was detected . - pubmed
Tryptophan halogenase which catalyses the regioselective chlorination of tryptophan to 7 - chlorotryptophan can also chlorinate tryptamine , tryptophol , indole-3 - acetonitrile , and 3 - methylindole . - pubmed
Presently , six members have been reported , these are : neprilysin , endothelin - converting enzyme ( ECE )- 1 and ECE-2 , the Kell blood group protein , PHEX ( product of the phosphate - regulating gene with homologies to endopeptidase on the X chromosome ) and X - converting enzyme ( XCE ) . - pubmed
In order to identify new members of this important family of peptidases , we designed a reverse transcriptase - PCR strategy based on conserved amino acid sequences of neprilysin , ECE-1 and PHEX . - pubmed
The recombinant enzyme exhibited neprilysin - like peptidase activity and was efficiently inhibited by phosphoramidon and thiorphan , two inhibitors of neprilysin . - pubmed
Both in vitro and in vivo studies of scavenger receptor class B type I ( SR - BI ) have implicated it as a likely participant in the metabolism of HDL cholesterol . - pubmed
The prominent anti - atherogenic effect of moderate SR - BI expression provides in vivo support for the hypothesis that HDL functions to inhibit atherogenesis through its interactions with SR - BI in facilitating reverse cholesterol transport . - pubmed
The amphipathic polyacid , poly ( 2 - ethylacrylic acid ) ( PEAA ) has recently been shown to form fluctuating channels in patch - clamp measurements of phospholipid bilayers ? - pubmed
The reaction path starting at the excited n - pi state of DMD is predicted to occur via a nonadiabatic mechanism , giving carbene and molecular dinitrogen ( both in their singlet ground states ) as the main products ; the computed barrier height ( 1.0 kcal mol(-)(1 ) ) agrees well with the experimental estimate of the activation energy in the singlet excited state ( 0.0 - 1.5 kcal mol(-)(1 ) ) . - pubmed
Linear IgA bullous dermatosis associated with vancomycin and disseminated varicella - zoster infection . - pubmed
It is known that NO is formed from L - arginine by NO synthase which is localized in neurons in the enteric nervous system . - pubmed
Although the primary stimulus regulating vasopressin ( VP ) release is a change in systemic osmolality , other physiological parameters are known to affect VP secretion or modulate the osmotic control over its release . - pubmed
The prominant features of uremic dyslipidemia are an increase in serum triglyceride levels ( due to elevated very low density lipoprotein [ VLDL]-remnants and intermediate - density lipoprotein [ IDL ] ) and low high - density lipoprotein ( HDL ) cholesterol . - pubmed
Low - density lipoprotein ( LDL ) cholesterol often is normal , but the cholesterol may originate from the atherogenic small and dense LDL subclass ( sdLDL ) . - pubmed
While LDL particles undergo a vicious cycle of accumulation and modification , reverse cholesterol transport is also impaired due to low lecithin : cholesterol acyltransferase ( LCAT ) and paraoxonase activity . - pubmed
Therefore , discoid HDL particles are structurally altered and hepatic cholesterol clearance is limited . - pubmed
Upon a light shift to PSII light , PSII , determined as Cyt b(559 ) per PBS , decreased rapidly , following the photoinactivation , down to the level a half of that before the light shift , and remained constant . - pubmed
Photoinactivation of PSII , and also decrease in Cyt b(559 ) , was accelerated , but only slightly , by the addition of chloramphenicol ( CAP ) at a moderate concentration while CAP at the same concentration significantly suppressed the increment of PSI determined as P700 . - pubmed
Mean glucose levels were significantly higher during the high - dose compared with the low - dose glucose infusion [ 9.5 + /- 0.8 vs. 6.8 + /- 0.2 mM ( P & lt ; 0.01 ) ] , resulting in increased mean insulin secretion rates [ ISRs ; 469.1 + /- 43.8 vs. 268.4 + /- 29 pmol / min ( P & lt ; 0.001 ) ] and mean insulin levels [ 213.6 + /- 46 vs. 67.9 + /- 10.9 pmol / l ( P & lt ; 0.008 ) ] . - pubmed
Spectral analysis evaluates the regularity of oscillations in glucose , insulin secretion , and insulin at a predetermined frequency . - pubmed
Spectral power for glucose , ISR , and insulin was reduced during the high - dose glucose infusion [ 11.8 + /- 1.4 to 7.0 + /- 1.6 ( P & lt ; 0.02 ) , 7.6 + /- 1.5 to 3.2 + /- 0.5 ( P & lt ; 0.04 ) , and 10.5 + /- 1.6 to 4.6 + /- 0.7 ( P & lt ; 0.01 ) , respectively ] . - pubmed
In conclusion , short - term infusion of high - dose glucose to obtain glucose levels similar to those previously seen in IGT subjects results in reduced spectral power for glucose , ISR , and insulin . - pubmed
Isolation of the protease nepenthacin . - pubmed
There was a positive correlation between the net output of lactate during the aortic cross - clamping period and postoperative Myo / CAIII ratio . - pubmed
Trichostatin A ( TSA ; 0 - 5 microM ) , a histone deacetylase inhibitor , was used to increase histone acetylation and 5 - aza-2 '- deoxycytidine ( 5 - aza - dC ; 0 - 5 microM ) , a DNA methyl - transferase inhibitor , was used to decrease methylation of chromatin in donor cells in an attempt to improve their reprogrammability . - pubmed
Adult fibroblast cells treated with 1.25 or 5 microM TSA had elevated histone H3 acetylation compared to untreated controls . - pubmed
In addition , 5 methods were used to test the construct validity of the RASS , including correlation with an attention screening examination ( r = 0.78 , P<.001 ) , GCS scores ( r = 0.91 , P<.001 ) , quantity of different psychoactive medication dosages 8 hours prior to assessment ( eg , lorazepam : r = - 0.31 , P<.001 ) , successful extubation ( P = .07 ) , and bispectral electroencephalography ( r = 0.63 , P<.001 ) . - pubmed
Molecular dissection of the S - adenosylmethionine - binding site of phosphatidylethanolamine N - methyltransferase . - pubmed
Phosphatidylethanolamine N - methyltransferase ( PEMT ) is a quatrotopic membrane protein that catalyzes the conversion of phosphatidylethanolamine to phosphatidylcholine through three sequential methylation reactions . - pubmed
Homocysteine is generated by the hydrolysis of S - adenosylhomocysteine , which is also a product of the PEMT reaction . - pubmed
To gain insight into the PEMT transmethylation reaction and the mechanism by which PEMT regulates homocysteine levels , we sought to define residues that are required for binding of the methyl group donor , S - adenosylmethionine ( AdoMet ) . - pubmed
Analysis of the AdoMet binding ability of mutant recombinant PEMT derivatives established that residues Gly100 and Glu180 are essential for binding of the AdoMet moiety . - pubmed
A model positions two separate AdoMet - binding motifs of PEMT in close proximity at the external leaflet of the endoplasmic reticulum membrane . - pubmed
Maximum growth of the tissue , as measured by dry weight , with the formation of collagen protein , based on the estimation of hydroxyproline , was obtained in periods of up to 6 days . - pubmed
Appreciable amounts of protein - bound hydroxyproline were formed during the first 24 hour growth period , but electron microscopy of portions of the same cultures failed to demonstrate the presence of any typical collagen fibrils . - pubmed
The subsequent formation of many characteristic collagen fibrils was not associated with a significant rise in the mean hydroxyproline content of the tissue . - pubmed
It is suggested that collagen - forming cells synthesise and secrete a hydroxyproline - rich precursor of protein or large peptide nature , which subsequently becomes directly transformed into typical collagen fibrils . - pubmed
[ Effect of histaminase on the secretion of gastric juice induced by histamine ] . - pubmed
Clone 7 - 3 - 5 - 5 was sequenced and found to contain a 672bp ORF encoding a 224 amino acid protein having a 19 amino acid signal sequence on the N - terminus and a 15 amino acid C - terminal GPI anchor site . - pubmed
Those agents that inhibit beta - adrenergic receptors ( class II beta - blockers , sotalol , and amiodarone ) have been demonstrated to be successful prophylaxis against postoperative AF . - pubmed
Preincubation with LY-294002 , a PI 3 - kinase inhibitor , or expression of dominant - negative Akt prevented ANG II - stimulated increase in VEGF translation . - pubmed
AIM : Despite the belief that silica ( Si ) is an inert and non - toxic ingredient , latest studies indicated that it is a potent mitochondria activator and Si - induced ROS generation is involved in the inflammatory reactions of silicotic lungs . - pubmed
GTP cyclohydrolase I ( GTPCH1 ) catalyzes the conversion of GTP to dihydroneopterin 3 '- triphosphate . - pubmed
These results suggest that 8 - oxo - GTP is a transition state analogue of GTPCH1 . - pubmed
We have solved the X - ray crystal structures of tGTPCH1 complexed with 8 - oxo - GTP and 8 - oxo - dGTP at 2.0 and 1.8 A resolution , respectively , as well as the free form of the enzyme at 2.2 A resolution . - pubmed
In the structure of tGTPCH1 complexed with 8 - oxo - GTP or 8 - oxo - dGTP , the oxygen atoms at O8 of the 8 - oxoguanine groups , together with residues Cys108 , His111 and Cys179 , are coordinated to the zinc ion . - pubmed
Superimpositioning of the structures indicates the imidazole ring of His110 is rotated , implying concomitant proton transfer to the ribose ring O4 ' . - pubmed
Both PrP(C ) and PrP(Sc ) have glycosylphospatidylinositol ( GPI ) anchors through which the protein is tethered to cell membranes . - pubmed
In order to study the effects of membranes on the structure of PrP , we synthesized a GPI anchor mimetic ( GPIm ) , which we have covalently coupled to a genetically engineered cysteine residue at the C - terminus of recombinant PrP. - pubmed
We show that the structure of PrP - GPIm reconstituted in phosphatidylcholine and raft membranes resembles that of PrP , without a GPI anchor , in solution . - pubmed
Increases in astrocytic Ca ( 2 + ) were a function of the frequency of stimulation , occurred within several seconds and were inhibited by metabotropic glutamate receptor antagonists . - pubmed
To distinguish between synaptic input and output , local synaptic activity in cortical layer 2 was silenced by iontophoresis of AMPA and NMDA receptor antagonists . - pubmed
Mutations of the BCR - ABL kinase domain are a common mechanism of resistance to imatinib in chronic myeloid leukemia . - pubmed
By multivariate analysis , factors associated with development of mutations were older age ( P=0.026 ) prior interferon therapy ( P=0.026 ) , and accelerated phase or blast phase at time of imatinib failure ( P=0.001 ) . - pubmed
The crystal structure determined by Rietveld analysis with the NPD data is of a distorted rutile type , similar to that of MoO2 ; space group P21 / c , a = 5.6891(1 ) , b = 4.7546(1 ) , c = 5.5195(1 ) A , and beta = 121.453(1 ) degrees . - pubmed
The NPD analysis also establishes a new neutron scattering length of 6.00(3 ) fm for 99Tc . - pubmed
The Tc - Tc bond was found to be 2.622(1 ) A from NPD profile analysis and 2.59 A from first - principles DFT calculations . - pubmed
The NPD results supported by the DFT calculations suggest that the filling of antibonding orbitals and the influence of the crystal field stabilization of the d3 Tc cations lead to more regular TcO6 octahedra and diminish the metal - metal bond strength compared with closely related oxides such MoO2 and WO2 . - pubmed
Synthesis of pyrrolo [ 2,3 - d ] pyridazinones as potent , subtype selective PDE4 inhibitors . - pubmed
A series of pyrrolo [ 2,3 - d ] pyridazinones was synthesized and tested for their inhibitory activity on PDE4 subtypes A , B and D and selectivity toward Rolipram high affinity binding site ( HARBS ) . - pubmed
Moreover the ratio HARBS / PDE4B was particularly favourable for 9e ( 147 ) , suggesting that the best arranged groups around the pyrrolopyridazinone core are an isopropyl at position-1 , an ethoxycarbonyl at position-2 , together with an ethyl group at position-6 . - pubmed
ParF , which exemplifies one clade of the ubiquitous ParA superfamily of segregation proteins , polymerizes extensively in response to ATP binding . - pubmed
Here , we establish that the ParF homologues of pTAR and pB171 filament modestly with ATP , and that nucleotide hydrolysis is not required for this polymerization , which is more prodigious when the cognate CBF is also present . - pubmed
By contrast , the ParF homologue of plasmid pVT745 did not respond appreciably to ATP alone , but polymerized extensively in the presence of both its cognate CBF and ATP . - pubmed
The co - factors also stimulated nucleotide - independent polymerization of cognate ParF proteins . - pubmed
2 - Aminoresorcinol is a potent alpha - glucosidase inhibitor . - pubmed
Glutamic acid decarboxylase ( GAD ) is the rate - limiting enzyme for gamma - aminobutyric acid ( GABA ) biosynthesis . - pubmed
To overcome these problems , this paper evaluates resistance and recovery , quantified by using a relative soil stability index ( RSSI ) , of the beta - glucosidase and protease activities towards an additional heat disturbance ( 17 h at 60 degrees C ) in soils where soil organic matter , pH and Cu content were modified in a factorial setup . - pubmed
Chemical analyses ( dissolved Cu , pCu ( 2 + ) , dissolved organic carbon , pH ) were performed both before the heat - perturbation and after the enzyme activity monitoring period . - pubmed
beta - glucosidase RSSI scores were scarcely affected by copper , making it inappropriate for evaluating copper - induced stress to soils . - pubmed
Thus , the protease RSSI score seems a good indicator for soil health relative to copper contamination given that all samples were affected by the presence of copper and high correlations were observed between RSSI scores and the different copper forms . - pubmed
Effect of charge distribution over a chlorophyll dimer on the redox potential of P680 in photosystem II as studied by density functional theory calculations . - pubmed
The effect of charge distribution over a chlorophyll dimer on the redox potential of P680 in photosystem II was studied by density functional theory calculations using the P680 coordinates in the X - ray structure . - pubmed
The novel HSP90 inhibitor STA-9090 exhibits activity against Kit - dependent and -independent malignant mast cell tumors . - pubmed
MATERIALS AND METHODS : BMCMCs , cell lines , and fresh malignant mast cells were treated with STA-9090 , 17 - AAG , and SU11654 and evaluated for loss in cell viability , cell death , alterations in HSP90 and Kit expression / signaling , and Kit mutation . - pubmed
RESULTS : Treatment of BMCMCs , cell lines , and fresh malignant cells with STA-9090 induced growth inhibition , apoptosis that was caspase-3 / 7 - dependent , and downregulation of phospho / total Kit and Akt , but not extracellular signal - regulated kinase ( ERK ) or phosphoinositide-3 kinase ( PI-3 K ) . - pubmed
Furthermore , STA-9090 exhibited superior activity to 17 - AAG and SU11654 , and was effective against malignant mast cells expressing either WT or mutant Kit . - pubmed
CONCLUSIONS : STA-9090 exhibits broad activity against mast cells expressing WT or mutant Kit , suggesting it may be an effective agent in the clinical setting against mast cell malignancies . - pubmed
Synthesis of collagen - like sequential polypeptides containing O - phospho - L - hydroxyproline and preparation of electrospun composite fibers for possible dental application . - pubmed
A synthetic route is described for collagen - like polypeptides constructed from O - phospho - L - hydroxyproline [ Hyp ( PO ( 3 ) H ( 2 ) ) ] residues . - pubmed
The initial rate and inhibitor analyses revealed that the mechanism of binding of three substrates , glutamate , ATP , and tubulin , to the enzyme was a random sequential pathway . - pubmed
Articular chondrocytes were subjected to several cell culture passages and treated with or without a Notch inhibitor , N -[ N -( 3 , 5 - difluorophenacetyl - L - alanyl )]- S - phenylglycine t - Butyl Ester ( DAPT ) . - pubmed
Furthermore , even though this TEM is driven by CXCR4 , the CXCR7 ligand CCX771 is substantially more potent at inhibiting it than the CXCR4 antagonist AMD3100 , which is more than 100 times weaker at inhibiting TEM when compared with its ability to block bare filter chemotaxis . - pubmed
Remarkably , the synthetic CXCR7 ligand CCX771 also potently stimulates beta - arrestin2 recruitment to CXCR7 , with greater potency and efficacy than the endogenous chemokine ligands . - pubmed
CXCR7 ligands , such as the chemokine CXCL11 and the newly described synthetic molecule CCX771 , may represent novel therapeutic opportunities for the control of such cells . - pubmed
In vitro , neurite outgrowth triggered by the Lewis(x-)carrying adhesion molecule CD24 was abolished alike by the Lewis ( x ) glycan and the glycomimetic peptide , while no influence of the glycomimetic peptide was seen in regeneration . - pubmed
If germination problems are bypassed by the addition of gibberellins , the seedlings of the quintuple phytochrome mutant exposed to red light produce chlorophyll , indicating that phytochromes are not the sole red - light photoreceptors , but they become developmentally arrested shortly after the cotyledon stage . - pubmed
We conclude that the degradation of the V - ATPases , electrogenic proton pumps on the tonoplast and the membranes of the Golgi- dependent secretory pathway , may be the cause for the cessation of growth in turgid coleoptiles and the associated loss of auxin sensitivity . - pubmed
Steaks were individually packaged in either vacuum ( VP ) , high - oxygen MAP ( HIOX ; 80 % O ( 2 )+20 % CO ( 2 ) ) , or carbon monoxide MAP ( CO ; 0.4 % CO+19.6 % CO ( 2 )+80 % N ( 2 ) ) , and stored for 0 , 5 , or 9 days at 1 C. At the end of storage , surface and internal colour ( visual and instrumental ) was measured on raw steaks . - pubmed
Interior cooked redness decreased ( P<0.05 ) during storage for steaks in VP and HIOX , whereas it was stable for steaks in CO . - pubmed
Besides hydrophobic interactions , the ligand 9 - cis retinoic acid interacts with RXRgamma through a hydrogen bond with Ala106 , a salt bridge with Arg95 and the pi - pi interactions with Phe217 and Phe218 . - pubmed
The binding modes exhibit some similarities among RXRs , such as the interactions with Arg95 and Ala106 . - pubmed
Nonetheless , owing to the absence of Ile47 , Cys48 , Ala50 , Ala51 and residues 225~237 in the active site , the binding pocket in RXRgamma is two times larger than those of RXRalpha and RXRbeta . - pubmed
Meanwhile , spatial effects of Trp84 , Arg95 , Ala106 , Phe217 and Phe218 help to create a differently shaped binding pocket as compared to those of RXRalpha and RXRbeta . - pubmed
GDF-15 levels correlated positively with estimated right ventricular systolic pressure on echocardiogram and plasma levels of the amino terminal propeptide form of brain natriuretic peptide . - pubmed
There was an inverse correlation between circulating GDF-15 and diffusing capacity of the lung for carbon monoxide ( Dlco ) and a positive correlation with the FVC to Dlco ratio on pulmonary function test . - pubmed
Upon subjecting the patterned silicon surfaces to solvothermal conditions , MOF thin films were obtained and characterized subsequently by AFM , SEM , and grazing - incidence XRD ( GIXRD ) . - pubmed
INTRODUCTION : Sirolimus is a potent immunosuppressant with a mechanism of action different from calcineurin inhibitors ( CNIs ) . - pubmed
METHODS : Between 2007 and 2009 , 250 newly diagnosed , histologically confirmed ccRCC cases and 299 sex- , age - matched healthy controls provided complete information including consumption of tea and alcohol , smoking , occupational exposure , body mass index ( BMI ) , hypertension , diabetes , and urolithiasis by face - to - face interview in Shanghai . - pubmed
Cannabidiol has been reported as a GPR55 antagonist in the literature to GPR55 activation by O1062 , but this could not be confirmed our laboratory ( Dr. Abood & amp ; Dr. Barak ) . - pubmed
There are no other known small molecule antagonists of GPR55 reported to date , only the CB1 inverse agonist / antagonists SR141716A ( 3.9 muM ) and AM251 ( 9.6 muM ) . - pubmed
We have identified 3 potent and selective antagonists for GPR55 , that represent 3 different chemical core scaffolds : 1 ) a quinoline aryl sulfonamide , ML193 ( CID1261822 ) with a 221 nM potency for GPR55 and & gt;145 - fold , & gt;27 - fold and & gt;145 - fold antagonist selectivity against GPR35 , CB1 and CB2 , respectively and & gt;145 - fold agonist selectivity against all of these counter - receptors ; 2 ) a thienopyrimidine , ML192 ( CID1434953 ) with 1080 nM potency for GPR55 and & gt;45 - fold antagonist and agonist selectivity against GPR35 , CB1 and CB2 ; and 3 ) a piperadinyloxadiazolone , ML191 ( 23612552 ) with 160 nM potency for GPR55 and & gt;100 - fold selectivity against GPR35 , CB1 and CB2 . - pubmed
When Taxol was locally injected near the germinal vesicle ( GV ) or the mitotic apparatus during meiosis I , PB formation was inhibited as mentioned below . - pubmed
In the oocytes , which were injected with Taxol after spindle formation , the spindle became large , and then the volume of the first PB also increased more than that of the control . - pubmed
Tissular hypoxia and its impact were quantified using pimonidazole immunostaining and mRNA of hypoxic inducible factor , vascular endothelial growth factor receptors 1 and 2 , Tie-2 , endothelial nitric oxide synthase , and inducible nitric oxide synthase . - pubmed
Fasting plasma glucose ( FPG ) , 2 - h plasma glucose ( 2hPG ) , HbA1c , insulin resistance ( HOMA2 - IR ) and beta - cell function ( HOMA2 - B ) were considered at 5 - year follow - up . - pubmed
We find that cells depleted of RECQL5 are sensitive to oxidative stress , accumulate endogenous DNA damage , and increase the cellular poly ( ADP - ribosyl )ate response . - pubmed
We recently identified a UVA phototransduction pathway in melanocytes that is mediated by G protein - coupled receptors and leads to rapid calcium mobilization . - pubmed
pullorum , denoted HPMG439 / HPMG440 , is involved in the control of nitrogen metabolism by regulating the expression of glutamate dehydrogenase , an AmtB ammonium transporter and a PII protein . - pubmed
Glucagon - like peptide-1 receptor agonist liraglutide inhibits endothelin-1 in endothelial cell by repressing nuclear factor - kappa B activation . - pubmed
To ascertain the role of NF - kappaB activation in the altered expression of ET-1 and eNOS , we treated HUVECs with phorbol 12 - myristate 13 - acetate ( PMA ) . - pubmed
PMA activated NF - kappaB and reversed the effects of liraglutide on eNOS and ET-1 expression . - pubmed
The effects of PMA on eNOS and ET-1 expression were reproduced in experiments wherein cells were treated with TNF - alpha . - pubmed
PMA increased IL-6 generation , and this effect was blocked by liraglutide . - pubmed
Genistein induces oestrogen receptor - alpha gene expression in osteoblasts through the activation of mitogen - activated protein kinases / NF - kappaB / activator protein-1 and promotes cell mineralisation . - pubmed
Oestrogen and oestrogen receptors ( ER ) play critical roles in the maintenance of bone remodelling . - pubmed
Sequentially , genistein increased the translocation of NF - kappaB and c - Jun from the cytoplasm to the nucleus . - pubmed
Therefore , the present study showed that genistein can induce ERalpha gene expression via the activation of MAPK / NF - kappaB / activator protein-1 and accordingly stimulates differentiation - related gene expressions and osteoblast mineralisation . - pubmed
Pre - treatment with the inhibitor , CRP - i2 , resulted in a significant reduction in CRP induced superoxide anion , NFkappab activity and biomarkers of inflammation . - pubmed
[ Detection of tumors in the central zone of the prostate with 11C - Choline PET / CT ] . - pubmed
In both , ( 11 ) C - Choline PET / CT allowed detection of the tumor focus in the central zone of the prostate , with negative complementary diagnostic test and biopsies . - pubmed
Part II continues this research by delving deeper into the amino acid , fatty acid and mineral components . - pubmed
Nanoporous ( NP ) gallium nitride ( GaN ) as a new class of GaN material has many interesting properties that the conventional GaN material does not have . - pubmed
In this paper , we focus on the mechanical properties of NP GaN , and the detailed physical mechanism of porous GaN in the application of liftoff . - pubmed
A decrease in elastic modulus and hardness was identified in NP GaN compared to the conventional GaN film . - pubmed
The promising application of NP GaN as release layers in the mechanical liftoff of GaN thin films and devices was systematically studied . - pubmed
A phase diagram was generated to correlate the initial NP GaN profiles with the as - overgrown morphologies of the NP structures . - pubmed
The fracture toughness of the NP GaN release layer was studied in terms of the voided - space - ratio . - pubmed
It is shown that the transformed morphologies and fracture toughness of the NP GaN layer after overgrowth strongly depends on the initial porosity of NP GaN templates . - pubmed
Exposure to chemotherapy prior to cell line initiation correlated with drug resistance of EFT cell lines in 5/8 tested agents at clinically achievable concentrations ( CAC ) or the lower tested concentration ( LTC ): ( cyclophosphamide ( as 4 - HC ) and doxorubicin at CAC , etoposide , irinotecan ( as SN-38 ) and melphalan at LTC ; P<0.1 for one agent , and P<0.05 for four agents . - pubmed
Neutron diffraction shows that the hydrides are best described as edge bridging rather than face bridging . - pubmed
By MD simulations we further investigated how VWD 2A / IID mutations might alter the ability of VWF to form carboxy - terminal dimers . - pubmed
PK 11195 , an isoquinoline analogue , is one of the ligands of highest TSPO binding affinity . - pubmed
The main water channel in the brain , aquaporin-4 ( AQP4 ) is involved in maintaining homeostasis and water exchange in the brain . - pubmed
In addition we used freeze - fracture electron microscopy to detect AQP4 in the form of orthogonal arrays of particles ( OAPs ) on the ultrastructural level . - pubmed
The cytochrome P450 monooxygenases are known to play a major role in pyrethroid resistance , by means of increased rate of insecticide detoxification as a result of their overexpression . - pubmed
leaves and roots was examined against benzyloxyresorufin O - debenzylation ( BROD ) of CYP6AA3 and CYP6P7 . - pubmed
Docking of flavones onto enzyme homology models reinforced relationships on flavone structures and inhibition modes . - pubmed
The SE - UBR group increased time spent in LPA by +20.68 + -29.30 minutes / day and the other groups decreased time spent in LPA by -22.43 + -47.88 minutes / day and -25.73 + -51.76 minutes / day . - pubmed
The Pn , stomatal limitation value ( Ls ) , maximal photochemical efficiency of PS II ( Fv / Fm ) , photochemical quenching coefficient ( q(p ) ) , content of malondialdehyde ( MDA ) , relative electric conductivity and superoxide dismutase ( SOD ) activity of leaves were very significantly correlated with the drought resistance coefficient . - pubmed
The self - propelled Janus silica micromotor catalytically decomposes a hydrogen peroxide fuel and moves along the direction of the catalyst face at a speed of 126.3 mum s(-1 ) . - pubmed
Sequence analysis of 5 isolates from the 4 provinces showed that they belonged to a relatively independent cluster that shared 2 insertions of a single amino acid in the gE gene and 1 insertion of 7 amino acids in the gC gene . - pubmed
The title compounds , C13H8ClN3O6 , ( I ) , and C13H9N3O6 , ( II ) , differ in the orientation of the two aromatic rings . - pubmed
The two nitro groups are essentially coplanar with the attached benzene rings , as indicated by the dihedral angles of 1.4 ( 2 ) and 2.3 ( 2 ) in ( I ) , and 4.96 ( 18 ) and 5.4 ( 2 ) in ( II ) . - pubmed
By following our previous introduction of PRM to targeting histone modifications ( Tang , H. ; Fang , H. ; Yin , E. ; Brasier , A. R. ; Sowers , L. C. ; Zhang , K. Multiplexed parallel reaction monitoring targeting histone modifications on the QExactive mass spectrometer . - pubmed
All received isonitrogenous , isocaloric PN [ 1.5 g / kg / d amino acids ( AAs ) and energy at 1.3x estimated basal energy expenditure ] . - pubmed
Controls ( n = 75 ) received standard GLN - free PN ( STD - PN ) ; the GLN group ( n = 75 ) received PN containing alanyl - GLN dipeptide ( 0.5 g / kg / d ) , proportionally replacing AA in PN ( GLN - PN ) . - pubmed
There were 11 hospital deaths ( 14.7 % ) in the GLN - PN group and 13 deaths in the STD - PN group ( 17.3 % ; difference , -2.6 % ; 95 % confidence interval , -14.6 % to 9.3 % ; P = 0.66 ) . - pubmed
The 6 - month cumulative mortality was 31.4 % in the GLN - PN group and 29.7 % in the STD - PN group ( P = 0.88 ) . - pubmed
Incident bloodstream infection rate was 9.6 and 8.4 per 1000 hospital days in the GLN - PN and STD - PN groups , respectively ( P = 0.73 ) . - pubmed
Orthovanadate ( OVA ) , a protein tyrosine phosphatase inhibitor , induces vasoconstriction in a Rho kinase - dependent manner . - pubmed
OVA - induced constriction of mesenteric arterial rings treated with N ( G )- nitro - L - arginine methyl ester ( L - NAME , 0.1 mM ) , a nitric oxide synthase inhibitor , was significantly blocked by the Rho kinase inhibitor Y-27632 ( R -(+)- trans - N -( 4 - pyridyl )- 4 -( 1 - aminoethyl )- cyclohexanecarboxamide , 10 microM ) , extracellular signal - regulated kinase 1 and 2 ( Erk1 / 2 ) inhibitor FR180204 ( 5 -( 2 - phenyl - pyrazolo [ 1,5 - a ] pyridin-3 - yl )- 1H - pyrazolo [ 3,4 - c ] pyridazin-3 - ylamine , 10 microM ) , Erk1 / 2 kinase ( MEK ) inhibitor PD98059 ( 2 '- amino-3 '- methoxyflavone , 10 microM ) , epidermal growth factor receptor ( EGFR ) inhibitor AG1478 ( 4 -( 3 - chloroanilino )- 6,7 - dimethoxyquinazoline , 10 microM ) , and Src inhibitor PP2 ( 4 - amino-3 -( 4 - chlorophenyl )- 1 -( t - butyl )- 1H - pyrazolo [ 3,4 - d ] pyrimidine , 3 microM ) . - pubmed
However , the myosin light chain kinase inhibitor ML-7 ( 1 -( 5 - iodonaphthalene-1 - sulfonyl )- homopiperazine , 10 microM ) did not affect OVA - induced constriction . - pubmed
The degree of formylation evaluated by ( 1 ) H NMR spectra was varied from 14.1 % to 37.2 % as a function of the reaction temperature . - pubmed
Effects of specific imidazoline receptor ( IR ) antagonists on the agmatine - induced relaxation were studied . - pubmed
The relaxation was inhibited by BU224 , a selective I2 IR antagonist ; but not by efaroxan ( I1 IR antagonist ) or KU14R ( I3 IR antagonist ) . - pubmed
Moreover , the agmatine - induced relaxation was attenuated by glibenclamide ( inhibitor of KATP channel ) and H-89 ( inhibitor of protein kinase A ) , but enhanced by 3 - isobutyl-1 - methylxanthine ( IBMX , inhibitor of cyclic AMP phosphodiesterase ) . - pubmed
BACKGROUND : Everolimus ( EVL)-based immunosuppressive strategies may permit the reduction of calcineurin inhibitors ( CNI ) and their side effects , while offering a safe and efficient treatment . - pubmed
omega - Imidazolyl - and omega - Tetrazolylalkylcarbamates as Inhibitors of Fatty Acid Amide Hydrolase : Biological Activity and in vitro Metabolic Stability . - pubmed
Fatty acid amide hydrolase ( FAAH ) is a serine hydrolase that terminates the analgesic and anti - inflammatory effects of endocannabinoids such as anandamide . - pubmed
Herein , structure - activity relationship studies on a new series of aryl N -( omega - imidazolyl - and omega - tetrazolylalkyl ) carbamate inhibitors of FAAH were investigated . - pubmed
Moreover , it was found that highly active pyridin-3 - yl carbamates reacted with albumin , which led to covalent albumin adducts . - pubmed
Most phenotypic correlations among Ca , P , Mg , and K concentrations were positive and low ( 0.09 - 0.16 ) , whereas the correlation between P and Mg was negative and low ( -0.16 ) . - pubmed
In this study , the differences in formation of Cu - mediated metal - organic coordination networks based on two pyridyl- and cyano - bearing free - base porphyrins on Ag ( 111 ) are elucidated by use of low - temperature scanning tunneling microscopy ( STM ) . - pubmed
Prior to the frozen donor egg transfer cycle , prednisone and intravenous immunoglobulin G ( IVIg ) treatment was initiated and patient achieved dichorionic diamniotic twin pregnancies . - pubmed
The present study was carried out to evaluate the capability of a polyethylene glycol mimetic technology as a biological alternative to improve pharmaceutical properties of human recombinant EPO . - pubmed
In silico models of EPO fused to 200 amino acids of proline , alanine , and serine ( PAS ) were initially generated and assessed by molecular dynamic ( MD ) simulation . - pubmed
Preclinical predictors that the orthosteric mGlu2 / 3 receptor antagonist LY3020371 will not engender ketamine - associated neurotoxic , motor , cognitive , subjective , or abuse - liability - related effects . - pubmed
The novel mGlu2 / 3 receptor antagonist , LY3020371 , has been shown to produce antidepressant - like effects comparable to that of the clinically - effective antidepressant ketamine . - pubmed
The robust polylysine protective shell makes the nanoparticles very stable under conditions of chemical etching , in the presence of glutathione , and at different pH values , without gold core deshielding or alteration of the SPR - like band . - pubmed
Susceptibility to rheumatoid arthritis ( RA ) is associated with HLA - DRB1 alleles coding a 5 - amino acid sequence motif called the shared epitope ( SE ) . - pubmed
The results also showed that CRME can reduce the levels of IL-1 , IL-6 , TNF - alpha , and PGE2 and inhibit the expression of NF - kappaB p65 . - pubmed
Compounds 3 and 7 exhibited anti - inflammatory activity through lowering the levels of TNF - alpha while 1 , 3 and 5 decreased the levels of neopterin better than the positive controls . - pubmed
The voltage - sensing residues in potassium channels have been extensively characterized , but current functional approaches do not allow a distinction between the electrostatic and steric contributions of the arginine side chain . - pubmed
Here we use chemical misacylation and in vivo nonsense suppression to encode citrulline , a neutral and nearly isosteric analogue of arginine , into the voltage sensor of the Shaker potassium channel . - pubmed
In contrast , substitution of the fourth S4 arginine with citrulline causes substantial changes in the conductance - voltage relationship and the kinetics of the channel , which suggests that a positive charge is required at this position for efficient voltage sensor deactivation and channel closure . - pubmed
In Functional magnetic resonance imaging ( fMRI ) , the blood oxygen level dependent ( BOLD ) signal is modeled as a convolution of the hemodynamic response function ( HRF ) and the unmeasured latent neural signal . - pubmed
Estrogen induces cell proliferation by promoting ABCG2 - mediated efflux in endometrial cancer cells . - pubmed
The present study aimed at understanding the regulatory mechanism underlying 17 - beta - estradiol ( E2)-induced cell proliferation under the control of ABCG2 . - pubmed
E2 - induced cell proliferation was inhibited by reserpine , an inhibitor of ABCG2 , and the ABCG2 siRNA treatment . - pubmed
Mitochondrial ultrastructure was observed by transmission electron microscope and adenosine triphosphate ( ATP ) content , sodium potassium ATPase ( Na / K - ATPase ) activity , and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day . - pubmed
CUMS reduced ATP content in hippocampus , decreased Na / K - ATPase activity and respiratory chain complex I , III , and IV activities in hippocampus and gastrocnemius muscle , and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle . - pubmed
XPKYF could partly reverse alterations in ATP , Na / K - ATPase , and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage . - pubmed
Using recombinant adenoviruses with amino acid substitutions or a deletion in the ribonucleoprotein motif the interaction of E1B 55kDa with RNA was found to correlate with timely and efficient viral DNA replication and viral late mRNA accumulation and splicing . - pubmed
Development of chitosan / glycerophosphate / collagen thermo - sensitive hydrogel for endoscopic treatment of mucosectomy - induced ulcer . - pubmed
In this study , we developed the chitosan / beta - glycerophosphate / collagen ( CS / GP / Col ) thermo - sensitive systems to satisfy the requirements for the endoscopic treatment for ESD - induced ulcer . - pubmed
In - silico design , ADMET screening , MM - GBSA binding free energy of some novel isoxazole substituted 9 - anilinoacridines as HER2 inhibitors targeting breast cancer . - pubmed
OBJECTIVE : A progression of novel isoxazole substituted 9 - anilinoacridines(1a - z ) were structured by in - silico technique for their HER2 inhibitory movement . - pubmed
RESULTS : Many compounds indicated solid hydrophobic communications and hydrogen holding associations to hinder HER2 . - pubmed
CONCLUSION : The outcomes reveals that , this examination gives proof to thought of profitable ligands in isoxazole substituted 9 - anilinoacridines as potential HER2 inhibitor and the compounds , 1s , x , v , a , j , r with noteworthy Glide scores may deliver critical enemy of bosom malignant growth movement for further examinations may demonstrate their remedial potential . - pubmed
Here , we tested the hypothesis that sedentary conditions , a major risk factor for CVD , increase GABAA receptors in RVLM , including its rostral extension ( RVLMRE ) , both of which contain bulbospinal catecholamine ( C1 ) and non - C1 neurons . - pubmed
These results suggest a complex interplay between GABAA receptor expression by spinally - projecting C1 and non - C1 neurons and sedentary versus physically active conditions . - pubmed
The released levels of phosphorylated - HSP27 was correlated with the levels of phosphorylated - HSP27 stimulated by ADP or collagen . - pubmed
The levels of ADP - induced phosphorylated - HSP27 were correlated with those of both phosphorylated - protein kinase B ( Akt ) and phosphorylatd - p38 mitogen - activated protein kinase ; however , the levels of phosphorylated - HSP27 stimulated by collagen were correlated with phosphorylated - Akt levels only . - pubmed
The ED50 value of ADP on the platelet aggregation in OSAS ( 1.067 + - 0.128 muM ) was lower than that in healthy subjects ( 1.778 + - 0.122 muM ) and was inversely correlated with both the value of minimum SpO2 and the released level of phosphorylated - HSP27 stimulated by ADP . - pubmed
Finally , we found aspirin alone had a lower live birth rate compared to LMWH plus aspirin ( OR=0.37 ; 95%CrI , 0.17 , 0.71 ) , and UFH plus aspirin showed a higher live birth rate than aspirin alone ( OR= 2.63 ; 95%CrI , 1.04 , 5.39 ) in NMA , Treating with UFH plus aspirin or LMWH plus aspirin did not have difference on live birth . - pubmed
Furthermore , LDA alone resulted in a lower birth weight compared to heparin plus aspirin , while higher birth weight was found when compared UFH plus aspirin to LMWH plus aspirin ( MD= 895.40 ; 95%CrI , 817.40 , 988.57 ) in NMA . - pubmed
Biocompatible long - circulating star carboxybetaine polymers . - pubmed
Here , we prepared four star carboxybetaine polymers of different molecular weights via atom transfer radical polymerization ( ATRP ) from a beta - cyclodextrin ( beta - CD ) initiator for investigating the biocompatibility of carboxybetaine polymer , a typical zwitterionic polymer . - pubmed
The internalization of the star carboxybetaine polymers by macrophage cells was a relatively slow process . - pubmed
The high cell viability in the presence of star carboxybetaine polymers up to 2 mg mL-1 was maintained . - pubmed
The hemolytic activity of the star carboxybetaine polymers at 5 mg mL-1 was almost undetectable . - pubmed
The anti - obesity effects of baicalin might be due to the up - regulation of solute carrier family 2 member 1 ( SLC2A1 ) and down - regulation of tumor necrosis factor ( TNF ) , nuclear factor kappa B subunit 1 ( NFKB1 ) , sterol regulatory element binding transcription factor 1 ( SREBF1 ) , peroxisome proliferator activated receptor gamma and caspase 3 ( CASP3 ) . - pubmed
Sirtuins ( SIRTs ) , a family of NAD+-dependent deacetylase , play an important role in regulating mitochondrial homeostasis and antioxidant defense by deacetylating transcription factors and enzymes such as PGC-1alpha , p65 , GCN5 , and SOD2 . - pubmed
However , age - related DNA damage and increased SASP activate PARP-1 and CD38 , the enzymes competing with SIRTs for NAD + . - pubmed
Aging increased the protein content of PARP-1 and CD38 , whereas decreased NAD + levels in both skeletal and heart muscles . - pubmed
Taken together , our data suggest that despite the upregulation of SIRTs , aged muscles suffered from NAD + deficit partly due to the competition of elevated CD38 and PARP-1 . - pubmed
A great preference for ordered binding is supported by stopped - flow double mixing experiments such that premixed CrAT with acetyl - CoA or CoA demonstrated a biphasic decrease in initial rate that produces about a 100 - fold attenuation in catalysis . - pubmed
Double mixing experiments also revealed that the CrAT initial rate is inhibited by 50 % in approximately 8 s by either acetyl - CoA or CoA premixing . - pubmed
Analysis of available CrAT structures support a substrate conformational change between acetyl - CoA / CoA binary versus ternary complexes . - pubmed
Enzyme LIP05 , belonging to the alpha / beta hydrolase family , was identified to synthesize short - chain fatty acid esters under aqueous phase . - pubmed
After deleting the lid domain of LIP05 , the synthesis of ethyl pentanoate , ethyl hexanoate , ethyl octanoate , or ethyl decanoate was achieved . - pubmed
Economic evaluation of adjuvant trastuzumab therapy for HER2 - positive early - stage breast cancer : systematic review and quality assessment . - pubmed
INTRODUCTION : As the availability of new economic evaluations ( EE ) on adjuvant trastuzumab therapy for early - stage breast cancer ( EBC ) with HER2 - positive since last search and other EEs missed warrant a more extensive review , this study aimed to systematically review EEs of adjuvant trastuzumab compared with chemotherapy alone for HER2 - positive EBC . - pubmed
Evidence shows cost - effectiveness of trastuzumab for HER2 - positive EBC in HICs . - pubmed
Therefore , to differentiate between these conditions , several dynamic tests , including those with desmopressin ( DDAVP ) , corticotrophin - releasing hormone ( CRH ) , and Dex / CRH have been developed . - pubmed
N - Ethylmaleimide prevents destruction of corticotropin ( ACTH ) in plasma . - pubmed
Addition of N - ethylmaleimide , an inhibitor of proteolytic enzymes , to samples for plasma corticotropin determinations prevents destruction of the hormone at room temperature for at least 72 h. A concentration of 125 mg per liter of blood is effective . - pubmed
N - Ethylmaleimide is not so effective in preventing corticotropin degradation in whole blood . - pubmed
CALLA reactivity and periodic acid Schiff ( PAS ) staining correlate poorly , with CALLA reacting with cells from 86 % ( 64 of 74 ) of patients with PAS - positive and 76 % ( 29 of 38 ) of those with PAS - negative non - T - cell ALL . - pubmed
The amino acid sequence of bovine cardiac tamponin - C. - pubmed
The factors related to increased fatality included hepatic cirrhosis , a combination of pneumonia and meningitis , complications such as shock , respiratory insufficiency , central nervous system disorders and circulatory acidosis , and laboratory findings such as thrombocytopenia , absence of leucocytosis and increased amounts of serum creatinine , aspartate aminotransferase and alanine aminotransferase on admission to the hospital . - pubmed
Impact of apo E phenotype on the regulation of cholesterol metabolism . - pubmed
E2/2 = 1 , E2/3 = 2 , E2/4 = 3 ... ) was negatively associated with cholesterol synthesis and fractional removal of LDL apo B and positively with the total and LDL cholesterol and LDL apo B concentrations . - pubmed
Reduction of fat and cholesterol consumption lowered while an increase of dietary cholesterol enhanced the LDL cholesterol concentration proportionately to the apo E subscript when cholesterol absorption efficiency was reduced in proportion to fat intake . - pubmed
Low absorption , effective elimination and high synthesis of cholesterol associated with low synthesis and effective removal of LDL apo B seem to be factors keeping serum cholesterol low during different diets . - pubmed
The findings also suggest that the apo E phenotypes regulate cholesterol metabolism during basal conditions and serum cholesterol responses to dietary modifications . - pubmed
Association of birdshot retinochoroidopathy and HLA - A29 antigen . - pubmed
However , this study seemed to confirm the need for a new approach to the intensive care of brain - dead subjects during lung procurement : the time elapsed before procurement should be shortened , the amount of medicinal solutions administered should be reduced , Ringer lactate and packed cells should be used in preference to albumin derivatives , and arterial blood pressure , rather than diuresis , should be maintained . - pubmed
We suggest that the hypotensive action of ketanserin is in part related to an interaction at alpha 1 - adrenoceptors . - pubmed
In contrast , contractions elicited during prolonged hypoxia were less sensitive to verapamil which at a concentration of 1 microM only caused a 16.2 + 2.2 % ( n 12 ) reduction of these responses . - pubmed
The human cytochrome Cyp1A2 gene contains regulatory elements responsive to 3 - methylcholanthrene . - pubmed
Deletion of flanking sequence to -1079 resulted in a loss of 3 - methylcholanthrene - induced chloramphenicol acetyltransferase activity . - pubmed
The strongest 3 - methylcholanthrene - induced chloramphenicol acetyltransferase activity , a 4 - fold increase , was observed for a DNA fragment located at -3202 to -1595 . - pubmed
These results suggest that the 3 - methylcholanthrene - responsive Cyp1A2 element may be regulated in a tissue - specific manner . - pubmed
Degradation and activation of parathion analogs by microsomal enzymes . - pubmed
Copper content in cervical mucus of women wearing plain and copper - bearing spring coils . - pubmed
Interaction of clonidine and clonidine analogs with human platelet alpha 2 - adrenergic receptors . - pubmed
The effects of clonidine and its p - amino analogs on platelet adenylate cyclase were also evaluated . - pubmed
This enzyme is inhibited by epinephrine acting via alpha 2 - adrenergic receptors . - pubmed
Both clonidine and p - aminoclonidine cause slight inhibition of basal adenylate cyclase and reverse the inhibition induced by epinephrine . - pubmed
These observations indicate that clonidine is a partial agonist for platelet alpha 2 - adrenergic receptors . - pubmed
If preparations of membrane fragments are incubated with [ gamma 32P ] ATP in the absence of type a1 protein kinase , until all membrane bound protein acceptor sites are saturated with phosphate addition of type a1 protein kinase causes the incorporation of more phosphate into the membrane proteins . - pubmed
The nature of the membrane proteins phosphorylated by type a1 protein kinase and the intrinsic protein kinase of the preparation was compared by SDS gel electrophoresis . - pubmed
Membrane preparations were phosphorylated with [ gamma 32P ] ATP in the absence of added type a1 protein kinase and with [ gamma 33P ] ATP in the presence of the enzyme . - pubmed
A comparison of the distribution of protein bound 33P and 32P made it quite clear that type a1 protein kinase catalysed the phosphorylation of different membrane proteins from those which are phosphorylated by the intrinsic protein kinase activity of the preparations . - pubmed
Vasopressin - dependent and -independent control of the release of adrenocorticotropin . - pubmed
We tested the possibility that vasopressin mediates the responses of adrenocorticotropin ( ACTH ) to electrical stimulation of various areas of the hypothalamus . - pubmed
Intraventricular administration of antiserum to vasopressin blocked the release of ACTH induced by electrical stimulation of the paraventricular nucleus ( PVN ) , suggesting a role for the vasopressinergic projection from PVN to the external zone of the median eminence . - pubmed
Thus , there is at least one corticotropin releasing factor released from nuclei other than PVN that is distinct from vasopressin . - pubmed
The cleavage of insulin by the partially purified insulin protease was studied using the four [ 125I ] tyrosine - monoiodoinsulins ( tyrosine A-14 and A-19 of the A - chain ; tyrosine B-16 and B-26 of the B - chain ) . - pubmed
Thus , the insulin protease appears to first degrade insulin to multiple products with molecular sizes slightly smaller than insulin and subsequently to small peptides ( e.g containing tyrosine A-19 ) and amino acids ( e.g. - pubmed
To assess the contribution of serum total proteins , albumin , cholesterol and immunoglobulin levels to low anion gap , a coefficient of correlation was calculated . - pubmed
The low anion gap correlated with low serum total proteins ( less than 4 g / dl ) and serum albumin levels ( less than 1.5 g / dl ) but not to serum cholesterol or immunoglobulin levels . - pubmed
An increase in ferritin concentration in serum was concurrent with decreased iron concentration . - pubmed
On the day the ovulatory dose of hCG was given , as determined by the first urinary estrogen value greater than 50 microgram/24 hours , estrogen excretion was similar whether single or multiple follicles of potential ovulatory size were present , but mean follicular diameters were significantly smaller when there was more than 1 follicle . - pubmed
Although ultrasound scan does not supplant estrogen monitoring , it can assist in the early detection of hyperstimulation , in the more accurate timing of the ovulatory dose of hCG , and in the withholding of hCG when 3 or more ovulations are possible . - pubmed
The formalin - fixed and paraffin - embedded buccal pouch sections were stained immunohistochemically with use of monoclonal antibodies for cytokeratins . - pubmed
Since the terminal carbohydrate moieties of some but not all of the plaques , which mainly consist of beta A4 protein , are identical to those of the microglial cells it is concluded that glycoproteins within these plaques have been synthesised by the microglial cell . - pubmed
As part of a program to prepare delta 8 ( 14 )- 15 - ketosterols that can not readily be metabolized to cholesterol or side - chain oxygenated species , we have prepared 3 beta - hydroxy-7 alpha - fluoro-5 alpha - cholest-8 ( 14 )- en-15 - one ( VII ) and the 9 alpha - hydroxy ( IV ) , 9 alpha - fluoro ( VI ) and 7 alpha - fluoro ( VIII ) derivatives of 3 beta - hydroxy-25,26,26,26,27,27,27 - heptafluoro-5 alpha - cholest-8 ( 14 )- en-15 - one ( II ) . - pubmed
Sterol IV was prepared by oxidation of the delta 8,14 dienol ethyl ether of the 3 beta - acetate of II with m - chloroperbenzoic acid , followed by mild alkaline hydrolysis of the 3 beta - acetate derivative of IV . - pubmed
Treatment of IV with hydrogen fluoride - pyridine gave VI . - pubmed
Chemical , biochemical , pharmacokinetic , and biological properties of L-680,833 : a potent , orally active monocyclic beta - lactam inhibitor of human polymorphonuclear leukocyte elastase . - pubmed
A series of potent and highly selective time - dependent monocyclic beta - lactam inhibitors of human polymorphonuclear leukocyte elastase ( PMNE , EC 3.4.21.37 ) is described . - pubmed
The intrinsic potency of these compounds , as exemplified by L-680,833 ( k(inactivation)/K(i ) of 622,000 M-1.s-1 ) , is reflected at the cellular level where it inhibits generation of the specific N - terminal cleavage product A alpha-(1 - 21 ) from the A alpha chain of fibrinogen by enzyme released from isolated polymorphonuclear leukocytes stimulated with fMet - Leu - Phe with an IC50 of 0.06 microM. - pubmed
The inhibitory activity of L-680,833 is also apparent in whole blood stimulated with A23187 , where it inhibits formation of A alpha -( 1 - 21 ) and PMNE - alpha 1 - proteinase inhibitor complex formation with IC50 values of 9 microM. - pubmed
The properties of L-680,833 allow it to effectively supplement the activity of natural inhibitors of PMNE in vivo , suggesting that this type of low - molecular - weight synthetic inhibitor could have therapeutic value in diseases where PMNE damages tissue . - pubmed
Moreover , a bivariate distribution of cells stained for DNA and cyclin B1 showed that , following melphalan and hyperthermia treatment , the fraction of cyclin B1 - expressing cells paralleled the fraction of G2+M phase cells , thus indicating that the inability of cells to enter mitosis was not ascribable to a reduction of cyclin B1 expression . - pubmed
After PDGF BB treatment , a 100 - kDa tyrosine phosphorylated species was detected in anti - Stat6 immunoprecipitates . - pubmed
Cycloheximide had little effect on Stat6 tyrosine phosphorylation . - pubmed
In addition to Stat6 , Stat5a , and Stat5b , PDGF BB also induced Jak1 tyrosine phosphorylation suggesting a potential pathway for Stat activation . - pubmed
Strikingly , the concurrent addition of IL-4 enhanced PDGF BB - induced Iepsilon binding activity , Jak1 tyrosine phosphorylation , and [ 3H ] thymidine incorporation . - pubmed
Heteromeric channel formation and Ca ( 2 +)-free media reduce the toxic effect of the weaver Kir 3.2 allele . - pubmed
We also demonstrate that the cell death induced by weaver expression may be prevented by elimination of calcium from the extracellular solution as well as by coexpression with the wild - type Kir 3.2 allele , or other members of the Kir 3.0 subfamily . - pubmed
These results suggest that the weaver defect in Kir 3.2 may cause cerebellar cell death by cell swelling and calcium overload . - pubmed
Myocardial beta - adrenoceptor density and plasma catecholamines in syndrome X. - pubmed
We performed positron emission tomography with ( 11 ) C - CGP-12177 to measure beta - adrenoceptor density . - pubmed
The C2 domain , also encoded by two exons , has an extra pair of cysteines . - pubmed
Inhibitory effects of anions and active site amino acid sequence of chicken liver L-2 - hydroxyacid oxidase A , a member of the FMN - dependent alpha - hydroxyacid oxidizing enzyme family . - pubmed
Monocarboxylic acids with aliphatic chains were found to be mixed inhibitors of chicken liver L-2 - hydroxyacid oxidase A when L-2 - hydroxy-4 - methylthiobutanoic acid was used as the substrate . - pubmed
The finding that the binding affinity of the enzyme for monocarboxylic acids was directly proportional to the number of carbon atoms in the chain strongly suggests that in addition to the electrostatic interaction due to the carboxyl moiety , hydrophobic forces may also be involved in the binding affinity of monocarboxylic acids to the enzyme 's active site . - pubmed
Oxalate , a dicarboxylic acid , also resulted in a mixed - type inhibition of chicken liver L-2 - hydroxyacid oxidase A , and , surprisingly , its binding affinity to the enzyme was found to be quite high as compared with monocarboxylic acids . - pubmed
Oxamate was found however to be a novel type of potent inhibitor of the enzyme . - pubmed
Blockage of mitotic apparatus formation by nocodazole also blocks the concentration of hsp70 molecules observed in nontreated eggs . - pubmed
Second , the percentage of CB- , CR- , and PV - ir neurons receiving multiple contacts on their somata and proximal dendrites from other calcium - binding protein neurons varied between 22 % and 85 % . - pubmed
Identification of an essential tyrosine residue in nitroalkane oxidase by modification with tetranitromethane . - pubmed
The enzyme is irreversibly inactivated by incubation with tetranitromethane , a tyrosine - directed reagent , at pH 7.3 . - pubmed
A saturation kinetic pattern is observed when the rate of inactivation is determined versus the concentration of tetranitromethane , indicating that a reversible enzyme - inhibitor complex is formed before irreversible inactivation occurs . - pubmed
The competitive inhibitor valerate protects the enzyme from inactivation by tetranitromethane , suggesting an active - site - directed inactivation . - pubmed
The UV - visible absorbance spectrum of the inactivated enzyme is perturbed with respect to that of the native enzyme , suggesting that treatment with tetranitromethane resulted in nitration of the enzyme . - pubmed
Comparison of tryptic maps of nitroalkane oxidase treated with tetranitromethane in the presence and absence of valerate shows a single peptide differentially labeled in the inactivated enzyme . - pubmed
After a confluency of 70 - 80 % was reached , cell proliferation was suppressed by arginine - deficient medium ( ADM ) , and UDS was assessed autoradiographically by 3H - thymidine incorporation upon exposure to OTA ( 10 - 2000 nM ) , ethyl methanesulfonate ( EMS , 5 mM , positive control ) , or dimethyl sulfoxide ( DMSO , 0.2 % , solvent control ) . - pubmed
In two HUC cultures derived from adults , a significant induction of UDS was observed in the concentration range of 50 - 500 nM OTA . - pubmed
The highest fraction of cells in repair ( CIR ) was found at 50 nM OTA for the HUC from the older male ( 50 % CIR ) . - pubmed
Liver hyperplasia and paradoxical regulation of glycogen metabolism and glucose - sensitive gene expression in GLUT2 - null hepatocytes . - pubmed
We investigated the impact of GLUT2 gene inactivation on the regulation of hepatic glucose metabolism during the fed to fast transition . - pubmed
This was correlated with glucose-6 - phosphate ( G6P ) levels , which remained at the fed values , indicating an important allosteric stimulation of glycogen synthase by G6P. - pubmed
These G6P levels were also accompanied by a paradoxical elevation of the mRNAs for L - pyruvate kinase . - pubmed
Together , these data indicate that in the absence of GLUT2 , the G6P levels can not decrease during a fasting period . - pubmed
Therefore , in fasting , GLUT2 is not required for quantitative normal glucose output but is necessary to equilibrate cytosolic glucose with the extracellular space . - pubmed
By examining the subcellular localization of GluRdelta2 mutants with various deletions in the carboxyl - terminal cytoplasmic region , we identified J segment adjacent to transmembrane segment M4 as a key region for the efficient targeting of GluRdelta2 to the plasma membrane . - pubmed
Our results suggest that the juxtamembrane segment and the PDZ domain target site at the carboxyl terminus of GluRdelta2 play differential roles in plasma membrane targeting , synapse localization and signal transduction . - pubmed
In three isolates , a mixture of resistant and susceptible subpopulations ( heteroresistance ) prohibited the detection of rpoB mutations in the initial analysis ; however , in these isolates , cluster I mutations could be verified after a passage on RIF - containing medium . - pubmed
The serum total triglyceride level was ( mean + /- SE ) 188.85 + /- 5.91 mg / dl at entry , and was also significantly decreased to 158.57 + /- 4.48 mg / dl at day 90 , this reduction was found statistically significant ( P & lt ; 0.01 ) . - pubmed
Whereas very - low density lipoprotein cholesterol reduced significantly from 33.08 + /- 1.09 mg / dl at day 0 to 31.02 + /- 0.95 mg / dl at day 90 ( P & lt ; 0.01 ) . - pubmed
Acarbose had no significant effect on serum total cholesterol , low - density lipoprotein cholesterol , and High - density lipoprotein cholesterol concentrations . - pubmed
Unlike Bcl-2 , however , the C - terminal membrane anchor region is preceded by a unique 250 amino acid insertion containing two tandem repeats . - pubmed
The AMPA - specific antagonist , GYKI 53655 , prevented a kainate - induced increase in ERG a- and d - waves , but was without effect on an increase in the b - wave . - pubmed
( 2 ) The kainate - evoked increase in ERG a- , b- , and d - waves probably results , in part , from an excitotoxic swelling of inner retinal processes . - pubmed
Sodium hypochlorite ( NaOCl ) addition was applied by most facilities as the temporary post - polishment step to comply with the color discharge standard . - pubmed
Clinic records were used to obtain patient characteristics , as well as independent variables including CD4 + count , coexisting hepatitis C and current alcohol use . - pubmed
A thyroid profile performed when he was admitted to our hospital showed several marked abnormalities : serum thyroid stimulating hormone ( TSH ) was elevated ( 69.4 microIU / ml : normal , 0.4 - 4.2 mIU / ml ) , free thyroxine level ( T4 ) ( 0.01 ng / dl ; normal , 0.70 - 1.60 ng / dl ) and free triiodothyronine level ( Ts ) ( 0.60 pg / ml ; normal , 2.3 - 4.1 pg / ml ) were low . - pubmed
However , pretreatment with N ( ) - nitro - L - arginine - methyl ester ( L - NAME ) , an inhibitor of nitric oxide ( NO ) synthase , significantly attenuated the inhibitory effect of E(2 ) . - pubmed
These results suggest that E(2 ) may inhibit spontaneous electrical activity of RVLM neurons , an effect which is mediated by the activation of NOS with the resultant of NO release via nongenomic actions . - pubmed
The full length ORF encodes a putative phosphoprotein containing a serine stretch and a bipartite nuclear localization signal , which we call Srsp1 ( " serin - rich spermatogenic protein 1 " ) . - pubmed
Development of an " electroptode " immunosensor : indium tin oxide - coated optical fiber tips conjugated with an electropolymerized thin film with conjugated cholera toxin B subunit . - pubmed
Avidin - biotin interactions were used to modify the fiber optics with biotin - conjugated cholera toxin B subunit molecules , for the construction of an immunosensor to detect cholera antitoxin antibodies . - pubmed
Identification of two histidines necessary for reovirus mRNA guanylyltransferase activity . - pubmed
The positive effect of low pH was detected for both autoguanylylation and GMP transfer , the two steps in the guanylyltransferase reaction . - pubmed
The effect of pH on enzymatic activity suggested that histidine protonation is responsible for the observed increase in guanylyltransferase activity . - pubmed
Mutagenesis of the two histidines conserved among the orthoreovirus and aquareovirus guanylyltransferases demonstrated that they are necessary for activity . - pubmed
The LNG - IUS down - regulates endometrial progesterone and estrogen receptors and this may play a role in the mechanism responsible for BTB . - pubmed
LNG is an androgenic progestogen and so we examined the regulation of the androgen receptor ( AR ) in endometrium exposed to intrauterine LNG . - pubmed
Furthermore , as the enzyme 17beta - hydroxysteroid dehydrogenase type 2 ( 17betaHSD2 ) regulates intracellular levels of estrogens , progestins and androgens , we evaluated the changes in expression of 17betaHSD2 in the same tissue endometrial samples . - pubmed
RESULTS : Immunohistochemistry showed that AR and 17betaHSD2 , which were immunolocalized to the stroma and glands of endometrium respectively , were both suppressed by LNG - IUS treatment , though moderate staining of 17betaHSD2 was evident 1 month after insertion of the LNG - IUS . - pubmed
AR mRNA expression was down - regulated in LNG - exposed endometrium when compared with the proliferative phase of the menstrual cycle . - pubmed
17betaHSD2 mRNA was significantly increased 3 months ( but not 6 - 12 months ) after LNG - IUS insertion . - pubmed
CONCLUSIONS : Endometrial intracellular estradiol levels would have been suppressed by 17betaHSD2 during the first few , but not the later , months of LNG - IUS action , and the lowered endometrial estradiol level may contribute to the frequent BTB evident in the early months of LNG - IUS use . - pubmed
The subsequent decline in 17betaHSD2 would lead to elevated local intracellular estradiol in the later months , when the BTB tends to subside . - pubmed
The suppression of AR by the LNG - IUS may also play a role in BTB , as elevated AR has been associated with amenorrhoea . - pubmed
Ribonucleotide reductase subunit M1 ( RRM1 ) is involved in DNA synthesis and repair and in gemcitabine metabolism , and the excision repair cross - complementing group 1 ( ERCC1 ) gene has been related to cisplatin activity . - pubmed
Thioacetamide - induced liver regeneration involves the expression of cyclooxygenase 2 and nitric oxide synthase 2 in hepatocytes . - pubmed
Analysis of cyclin D1 , E and PCNA correlated with hepatocytes entering into the S phase of cell cycle by the effect of TAM . - pubmed
However , the absence of NO delays hepatocyte regeneration , whereas COX-2 - inhibition appears to decrease liver damage . - pubmed
Effects of idoxifene on antioxidant enzyme levels of copper , zinc - dependent superoxide dismutase ( CuZn - SOD ) , and cellular glutathione peroxidase ( GSHPx ) were measured by ELISA . - pubmed
A treatment of 0.4 mg x kg(-1 ) x d(-1 ) of idoxifene reduced the protein levels of collagen in the DMN model by 41.19 % ( P<0.05 ) . - pubmed
Protein level of CuZn - SOD and activitiy of GSHPx in liver treated with DMN plus 0.4 mg / kg / d of idoxifene were 2.65 times ( P<0.05 ) and 2.08 times greater ( P<0.05 ) than that of liver treated with DMN alone respectively . - pubmed
We have shown that nocturnin acts as a deadenylase in vitro , removing the poly(A ) tail from a mature messenger RNA in a process that either leads to degradation or translational silencing of a message . - pubmed
The endoplasmic reticulum ( ER ) is a fundamental organelle required for protein assembly , lipid biosynthesis , and vesicular traffic , as well as calcium storage and the controlled release of calcium from the ER lumen into the cytosol . - pubmed
The increased expression of eNOS in dura mater and eNOS and nNOS in pia mater seen after stress could not be antagonized by treatment with the migraine drug sumatriptan . - pubmed
The effect of mold temperature on castability of CP Ti and Ti-6Al-4V castings into phosphate bonded investment materials . - pubmed
OBJECTIVE : The aim of this study was to evaluate the castability of CP titanium and Ti-6Al-4V alloy castings into Rematitan Plus investment at three different mold temperatures . - pubmed
RESULTS : The Ti-6Al-4V alloy ( 60.86 % ) presented a better castability index than CP Ti ( 48.44 % ) ( p & lt ; 0.000001 ) . - pubmed
For CP Ti , the temperature of 530 degrees C ( 23.96 % ) presented better castability than at other temperatures , 480 degrees C ( 14.66 % ) and 430 degrees C ( 12.54 % ) , with no difference between them ( p & lt ; 0.001 ) . - pubmed
SIGNIFICANCE : Within the limitations of this study , the increase in the mold temperature of the Rematitan Plus investment resulted in a better castability index for both materials , and Ti-6Al-4V presented a better castability index than CP Ti . - pubmed
Vasopressors : 1 mg epinephrine every 3 - 5 min IV . - pubmed
Epinephrine 10 microg / kg IV or intraosseously , or 100 microg ( endobronchially ) every 3 - 5 min . - pubmed
In parallel to pain therapy , aspirin ( 160 - 325 mg PO or IV ) and in addition clopidogrel ( 300 mg PO ) . - pubmed
As antithrombin , heparin ( 60 IU / kg , max . - pubmed
After hemorrhage exclusion , thrombolysis within 3 h of symptom onset ( 0.9 mg / kg rt - PA IV ; max 90 mg within 60 min , 10 % of the entire dosage as initial bolus , no aspirin , no heparin within the first 24 h ) . - pubmed
Using this in vitro assembly model , it was found that at a molar ratio of 1:1 ; LDS47 : EtxB , 50 % of reassembly was inhibited , and that this inhibition increased to 90 % at a ratio of 2:1 . - pubmed
Perfusion with the GABA ( A ) receptor antagonist picrotoxin ( 100 microM ) increased KCl - evoked effect , whereas NMDA and non - NMDA glutamate receptor antagonists ( D - AP5 100 microM and CNQX 30 microM , respectively ) were able to decrease KCl - evoked effect only in the presence of picrotoxin ( 100 microM ) . - pubmed
Bath application of WIN 55212 - 2 ( 10 microM ) inhibited KCl - evoked effect ; this inhibition was blocked by the CB ( 1 ) receptor antagonist AM 251 ( 1 microM ) . - pubmed
In conclusion , GABAergic and glutamatergic components are both involved in KCl - evoked excitation of LC neurons , although CB ( 1 ) receptors only seem to inhibit the glutamatergic component of KCl effect in the locus coeruleus . - pubmed
RT - PCR and immunoblotting experiments showed that almost all the PDE4D isoforms , known as the main cAMP - regulated rolipram - sensitive PDE in Sertoli cells , were expressed throughout the early postpartum period , whereas only the short PDE4D isoforms ( PDE4D1 and PDE4D2 ) were transcriptionally regulated by FSH . - pubmed
Resensitization of the cAMP response to FSH in 20 - day - old Sertoli cells was also associated with the highest FSH - induced transient increase in both soluble and particulate PDE4 activities , which suggests developmental changes in the PKA - mediated upregulation of the catalytic activities of long PDE4D. - pubmed
Nineteen well characterised cases were further submitted to immunohistochemical studies for expression of YF antigen , hepatitis B surface antigen ( HBsAg ) and delta virus antigen . - pubmed
This study focused on the behavior of topoisomerase IIalpha in response to oxidative stress induced by hydrogen peroxide ( H ( 2 ) O ( 2 ) ) . - pubmed
In whole cell voltage clamp , activation of PKC by application of 1 - oleoyl-2 - acetyl - sn - glycerol ( OAG , 10 - 30 microM ) resulted in 1 ) a reduction of I(T ) amplitude by 33 % accompanied an increase in half - width and a decrease in the maximal rise and decay rates of the I(T ) ; 2 ) a reduction of PIC amplitude by 49 % , with a depolarization of PIC onset by 4.5 mV. - pubmed
Inclusion of PKCI19 - 36 ( 10 - 30 microM ) , a PKC inhibitor , in the recording pipette could block the OAG effects on I(T ) and PIC . - pubmed
The ability of serotonin to hyperpolarize Vth was not altered by PKC activation or inhibition . - pubmed
Nylon-6 capillary - channeled polymer ( C - CP ) fibers as a hydrophobic interaction chromatography stationary phase for the separation of proteins . - pubmed
Nylon-6 capillary - channeled polymer ( C - CP ) fibers are used as the stationary phase for the hydrophobic interaction chromatography ( HIC ) separation of a synthetic protein mixture composed of ribonuclease A , lysozyme , and holotransferrin . - pubmed
In conclusion , voluntary wheel running failed to modify sensitivity to cardiovascular action of oxytocin but resulted in increased gene expression of PNMT in left , but not right , heart atrium in a running activity - dependent manner . - pubmed
Drugs that slow conduction through the AV node ( such as digoxin , beta blockers and calcium channel blockers ) have been used as adjuvants to therapy for the prevention of atrial fibrillation . - pubmed
Membrane gamma - glutamyl transferase activity promotes iron - dependent oxidative DNA damage in melanoma cells . - pubmed
A number of recent observations have suggested a potential role for membrane - bound gamma - glutamyltransferase ( GGT ) in tumor progression and appearance of more aggressive and resistant phenotypes , through redox interactions leading to production of reactive oxygen species . - pubmed
Our results indicate that higher levels of GGT activity are associated with higher levels of background DNA damage and oxidized bases . - pubmed
GGT - over - expressing cells also presented with a markedly higher glucose uptake , a phenomenon potentially leading to higher metabolic rate and oxidative DNA damage . - pubmed
Anyway , when GGT - over - expressing cells were incubated in the presence of GGT substrates and a source of catalytic iron , increased levels of DNA damage and oxidized bases were observed , an effect completely prevented in the presence of GGT inhibitors or various antioxidants . - pubmed
The findings reported indicate that GGT activity is able to promote iron - dependent DNA oxidative damage , thus potentially representing an important mechanism in initiation / progression of neoplastic transformation . - pubmed
To investigate the influence of the different linkage groups on the final monolayer structure , organic monolayers were prepared from 1 - alkenes and 1 - alkynes with chain lengths from C ( 12 ) to C ( 18 ) , and the final monolayer structures were studied in detail by static water contact angles measurements , ellipsometry , attenuated total reflectance infrared ( ATR - IR ) spectroscopy , and X - ray photoelectron spectroscopy ( XPS ) . - pubmed
Penehyclidine hydrochloride alone has no effect on learning and memory abilities and the synaptophysin expression . - pubmed
PREDICTOR : Serum glucose and HbA ( 1c ) levels . - pubmed
LIMITATIONS : Registry data ; casual serum glucose measurements ; HbA ( 1c ) values available for only a subset of participants . - pubmed
CONCLUSIONS : Higher casual glucose and HbA ( 1c ) levels were not associated with mortality in maintenance hemodialysis patients with or without diabetes . - pubmed
Based on their surface markers , cytokine production , and ability to prime naive allogenic T cells , we found that purified BDCA-2(+)BDCA-4(+ ) pDCs consist of at least two separate populations , which differed in their response to oligodeoxynucleotides and IFNs ( IFN - beta ) , and differently induced IL-17- or IL-10 - producing T cells . - pubmed
Retrospective , observation study : Quantitative and qualitative effect of ezetimibe and HMG - CoA reductase inhibitors on LDL - cholesterol : are there disappearance thresholds for small , dense LDL and IDL ? - pubmed
The correlation was between LDL - C and small , dense LDL as well as mid - band lipoprotein cholesterol . - pubmed
BACKGROUND : EphrinA5 is one of the best - studied members of the Eph - ephrin family of guidance molecules , known to be involved in brain developmental processes . - pubmed
Considering that this membrane - anchored molecule may act far from the neuron soma expressing the transcript , it is of a crucial interest to localize ephrinA5 protein to better understand its function . - pubmed
Plasminogen is a precursor to the fibrinolytic enzyme plasmin and is known to undergo large conformational changes when subjected to low molecular lysine analogues such as tranexamic acid ( TA ) or epsilon - amino - n - caproic acid ( EACA ) . - pubmed
The interaction was studied by the quartz crystal microbalance with dissipation monitoring ( QCM - D ) technique as well as by surface plasmon resonance ( SPR ) based sensing . - pubmed
Upon binding of TA or EACA , changes in the plasminogen structure were recorded as distinct , although small , DeltaD responses which were used to determine affinity constants . - pubmed
BACKGROUND / AIM : this study evaluated esophageal radioprotection by the Gramicidin S ( GS ) derived - nitroxide , JP4 - 039 , a mitochondrial targeting peptide - isostere covalently - linked to 4 - amino - Tempo , delivered in a novel swallowed oil - based ( F15 ) formulation . - pubmed
RESULTS : JP4 - 039 / F15 or MnSOD - PL increased survival compared to irradiated controls ( p<0.0001 for either ) . - pubmed
Among the genes altered in expression level in AD brains was a transcript encoding a novel protein , SDIM1 , that contains 146 amino acids , including a typical signal peptide and two transmembrane domains . - pubmed
HLA class I antigens were typed serologically using microlymphocytotoxicity technique . - pubmed
Under the optimized conditions [ ECL detection at 1.15 V , 25 mmol / L phosphate buffer solution ( PBS ) , pH 8.0 , as running buffer , separation voltage 12.5 kV , 5 mmol / L Ru ( bpy ) 32 + with 60 mmol / L PBS , pH 8.5 , in the detection cell ] linear correlation ( r = 0.9987 ) between ECL intensity and ephedrine concentration was obtained in the range 6.0 x 10 - 8 - 6.0 x 10 - 6 g / mL. - pubmed
The number of binding sites and the binding constants between ephedrine and BSA , Cyt - C and Mb were 8.52 , 12.60 , 10.66 and 1.55 x 104 mol / L , 6.58 x 103 mol / L and 1.59 x 104 mol / L , respectively . - pubmed
Structure and function of glutamate receptor amino terminal domains . - pubmed
The amino terminal domain ( ATD ) of ionotropic glutamate receptor ( iGluR ) subunits resides at the extracellular region distal to the membrane . - pubmed
In this study , we generated a caspase-1 - ESC line transfected with a mifepristone - regulated caspase-1 expression system . - pubmed
Mifepristone induced caspase-1 overexpression both in differentiated and undifferentiated caspase-1 - ESCs . - pubmed
All the undifferentiated caspase-1 - ESCs were induced to death after mifepristone treatment . - pubmed
Tumors derived from undifferentiated caspase-1 - ESCs were eliminated following 3 weeks of mifepristone treatment in vivo . - pubmed
However , differentiated caspase-1 - ESCs survived well under the condition of mifepristone - induced caspase-1 overexpression . - pubmed
These data demonstrate that mifepristone - induced caspase-1 overexpression in ESCs can eliminate the potential tumor formation meanwhile spares the differentiated cells in the host brains . - pubmed
Reference measurement procedure for total glycerides by isotope dilution GC - MS . - pubmed
BACKGROUND : The CDC 's Lipid Standardization Program established the chromotropic acid ( CA ) reference measurement procedure ( RMP ) as the accuracy base for standardization and metrological traceability for triglyceride testing . - pubmed
To overcome these problems the CDC developed an isotope dilution GC - MS ( ID - GC - MS ) RMP for total glycerides in serum . - pubmed
The samples were dried , derivatized with acetic anhydride and pyridine , extracted with ethyl acetate , and analyzed by ID - GC - MS . - pubmed
CONCLUSIONS : This ID - GC - MS RMP has the specificity and ruggedness to accurately quantify total glycerides in the serum pools used in the CDC 's Lipid Standardization Program and demonstrates sufficiently acceptable agreement with the NIST primary RMP for total glyceride measurement . - pubmed
Effects of ethanol on CYP2E1 levels and related oxidative stress using a standard balanced diet . - pubmed
Expression of cytochrome P4502E1 ( CYP2E1 ) is very much influenced by nutritional factors , especially carbohydrate consumption , and various results concerning the expression of CYP2E1 were obtained with a low - carbohydrate diet . - pubmed
This study describes the effects of ethanol treatment on CYP2E1 levels and its relationship with oxidative stress using a balanced standard diet to avoid low or high carbohydrate consumption . - pubmed
The results have shown that ethanol administration was associated with CYP2E1 induction and stabilization without related oxidative stress . - pubmed
Our findings suggest that experimental models with a low - carbohydrate / high - fat diet produce some undesirable CYP2E1 changes that are not present when a balanced standard diet is given . - pubmed
Protein hydrogen / deuterium exchange ( HDX ) followed by protease digestion and mass spectrometric ( MS ) analysis is accepted as a standard method for studying protein conformation and conformational dynamics . - pubmed
The utility of the HDX MS platform is demonstrated by exploring the conformational transition of a monoclonal antibody by increasing concentrations of guanidine . - pubmed
Based on spatial interpolation data from 2003 to 2010 , combined with almost 30 years of nitrogen and phosphorus pollutant data , this research analyzed the variations in total nitrogen ( TN ) , nitrate nitrogen ( NO ( 3 )- N ) , total phosphorus ( TP ) and phosphate - P ( PO ( 4 )- P ) in the Yangtze River Estuary ( YRE ) . - pubmed
Role of the phosphatidylinositol 3 - kinase and extracellular signal - regulated kinase pathways in the neuroprotective effects of cilnidipine against hypoxia in a primary culture of cortical neurons . - pubmed
Cilnidipine , a calcium channel blocker , has been reported to have neuroprotective effects . - pubmed
We investigated whether cilnidipine could protect neurons from hypoxia and explored the role of the phosphatidylinositol 3 - kinase ( PI3 K ) and extracellular signal - related kinase ( ERK ) pathways in the neuroprotective effect of cilnidipine . - pubmed
The viability of a primary culture of cortical neurons injured by hypoxia , measured by trypan blue staining and lactate dehydrogenase ( LDH ) assay , was dramatically restored by cilnidipine treatment . - pubmed
Survival signaling proteins associated with the PI3 K and ERK pathways were significantly increased while death signaling proteins were markedly decreased in the primary culture of cortical neurons simultaneously exposed to cilnidipine and hypoxia when compared with the neurons exposed only to hypoxia . - pubmed
These neuroprotective effects of cilnidipine were blocked by treatment with a PI3 K inhibitor or an ERK inhibitor . - pubmed
These results show that cilnidipine protects primary cultured cortical neurons from hypoxia by reducing free radical stress , calcium influx , and death - related signaling proteins and by increasing survival - related proteins associated with the PI3 K and ERK pathways , and that activation of those pathways plays an important role in the neuroprotective effects of cilnidipine against hypoxia . - pubmed
We showed EcdI activates linoleate as linoleyl - AMP and installs it on the first thiolation domain of EcdA. - pubmed
We have also established through ATP - PP(i ) exchange assay that EcdA loads L - ornithine in the first module . - pubmed
A separate hty gene cluster encodes four enzymes for de novo generation of L - homotyrosine from acetyl - CoA and 4 - hydroxyphenyl - pyruvate is found from the sequenced genome . - pubmed
Deletions in the ecdA , and htyA genes validate their essential roles in echinocandin B production . - pubmed
Five predicted iron - centered oxygenase genes , ecdG , ecdH , ecdK , htyE , and htyF , in the two separate ecd and hty clusters are likely to be the tailoring oxygenases for maturation of the nascent NRPS lipohexapeptidolactam product . - pubmed
Urinary chromium excretion in response to an insulin challenge is not a biomarker for chromium status . - pubmed
Cr is known to be mobilized in the body in response to insulin ( or insulin release in response to a glucose challenge ) , resulting in an increase in urinary Cr excretion . - pubmed
Urine samples were collected before and after insulin and glucose challenges ( 0 , 2 , 6 , and 12 h postinjection ) . - pubmed
Overall , the study demonstrated that insulin - stimulated urinary Cr excretion can not be used as a biomarker for Cr status . - pubmed
The androgen metabolite , 5alpha - androstane-3beta,17beta - diol ( 3beta - diol ) , activates the oxytocin promoter through an estrogen receptor - beta pathway . - pubmed
Therefore , a possibility is that the PVN target neurons respond to a metabolite in the testosterone catabolic pathway via an AR - independent mechanism . - pubmed
The dihydrotestosterone metabolite , 5alpha - androstane-3beta,17beta - diol ( 3beta - diol ) , binds and activates estrogen receptor - beta ( ER - beta ) , the predominant ER in the PVN . - pubmed
In the PVN , ER - beta is coexpressed with oxytocin ( OT ) . - pubmed
Therefore , we tested the hypothesis that 3beta - diol regulates OT expression through ER - beta activation . - pubmed
In a dose - dependent manner , 3beta - diol treatment increased OT - luc activity when cells were cotransfected with ER - beta , but not ER - alpha . - pubmed
The 3beta - diol - induced OT - luc activity was reduced by deletion of the promoter region containing the composite hormone response element ( cHRE ) . - pubmed
Point mutations of the cHRE also prevented OT - luc activation by 3beta - diol . - pubmed
These results indicate that 3beta - diol induces OT promoter activity via ER - beta - cHRE interactions . - pubmed
Potential prognostic factors ( pre- and post - implant dosimetric indices , GS 3 + 4 versus 4 + 3 , androgen deprivation therapy ( ADT ) ) were analysed . - pubmed
insulin and glucose ) as an independent concentration ( of e.g. - pubmed
, the largest possible decrease in glucose predicted by the model is smaller than reported decreases , and the model predicts no decrease whatsoever in glucose xinsulin , in contrast to large observed decreases in homeostatic model assessment insulin resistance ( HOMA - IR ) . - pubmed
On the other hand , both effects can be accounted for if the surgery leads to a substantial increase in some substance that opens an alternative insulin - independent pathway for glucose transport into muscle cells , which perhaps uses the same intracellular pool of GLUT-4 that is employed in an established insulin - independent pathway stimulated by muscle contraction during exercise . - pubmed
However , the present results suggest that another possible mechanism should be added to the list of candidates : enhanced production in the lower intestine of a substance which opens an alternative insulin - independent pathway for glucose transport . - pubmed
The production of nitric oxide ( NO ) , the expression of metalloprotease-2 ( MMP-2)/tissue inhibitor metalloprotease-2 ( TIMP-2 ) , and the activation of MMP-2 were measured under the different levels of cyclic equibiaxial deformation either in the presence or absence of TNF - alpha . - pubmed
Our study indicated that cyclic equibiaxial deformation - induced production of NO and MMP-2 / TIMP-2 . - pubmed
An N - terminal - domain ( NTD ) and adjacent catalytic body ( CB ) make up subunit - alpha of ribonucleotide reductase ( RNR ) , the rate - limiting enzyme for de novo dNTP biosynthesis . - pubmed
A strong linkage exists between ligand binding at the NTD and oligomerization - coupled RNR inhibition , inducible by both dATP and nucleotide chemotherapeutics . - pubmed
Both the NTD and the CB of the HE bind dATP . - pubmed
However , although the NTD is the sole physical tether to support alpha2 and/or beta2 associations in the dATP - bound alpha6 or alpha4beta4 fully inhibited RNR complexes , the binding of dATP to the HE NTD only partially suppresses HE activity and fully precludes formation of higher - order HE oligomers . - pubmed
Efflux of rhodamine 123 or calcein - acetoxymethylester was used to study the P - gp profile . - pubmed
P - gp - overexpressing National Cancer Institute / Adriamycin - Resistant cells were also sensitive to B014 . - pubmed
B014 - induced loss of Mcl-1 was accompanied by activation of caspases-3 , - 7 , - 8 and - 9 , and initiation of apoptosis . - pubmed
Inspection of these residues ' spatial arrangement in the active site , allowed us to conclude that PncC is a serine - amidohydrolase , employing a Ser / Lys dyad for catalysis . - pubmed
Analysis of the PncC structure in complex with a modeled NMN substrate supported our conclusion , and enabled us to propose the catalytic mechanism . - pubmed
In adipose , the most activated pathways by SCE were nicotinate and nicotinamide metabolism , long - chain fatty acid transport , oxidative phosphorylation , inflammation , T cell and B cell receptor signaling , and mTOR signaling . - pubmed
TiNT were synthesized via hydrothermal treatment of titania powders in NaOH solution at 130 C for 48 h. The obtained samples were characterized by UV - Vis absorption spectroscopy , Brunauer - Emmett - Teller ( BET ) surface area , XRD , TEM , XPS , and TG analysis . - pubmed
MEK is very resistant to photocatalytic degradation with the prepared materials ; , however , the results show that modification of the TiNT with nitrogen in a proportion of 1 to 1 ( TiNT to urea weight ratio ) and calcination at 400 C lead to materials with high photocatalytic activity under ultraviolet radiation and moderate photocatalytic activity under solar radiation for degradation of H2S. - pubmed
EGFR modulates DNA synthesis and repair through Tyr phosphorylation of histone H4 . - pubmed
Here , we identify a tyrosine phosphorylation site at Y72 of histone H4 , which facilitates recruitment of histone methyltransferases ( HMTases ) , SET8 and SUV4 - 20H , to enhance its K20 methylation , thereby promoting DNA synthesis and repair . - pubmed
We designed an experimental , open , prospective and comparative study , divided in seven groups : 1 - BMP-2+calcium phosphate ( BT ) ; 2 - BMP-2+acellular dermal matrix ( BM ) ; 3 - BMP-2+calcium alginate ( BA ) ; 4 - TCP ; 5 - MDM ; 6 - ALG ; 7 - Bone autograft ( BAG ) . - pubmed
CONCLUSIONS : Bone morphogenetic protein type 2 increased bone regeneration in experimental skull reconstruction , especially when combined to calcium phosphate . - pubmed
AIM : The aim of this study was to determine whether the sphenovomerine suture ( SVS ) can be used as a landmark to localize the sphenoidal sinus ostium . - pubmed
Based on the new model , the polarity of the electronic ground state ( S0 ) in indoles is very similar to that of the first electronic excited state ( S1 ) producing this structured emission ; however , this excited state can lead to a highly dipolar excited state ( S1 ' ) with largely structureless emission under the influence of the polarity of the medium , and also , very likely , of its viscosity . - pubmed
Thus , the polarized emission spectra for indole in glycerol at 283 K and 223 K showed the transition dipole moments for the emission from the first two excited states in a polar medium , S1 and S1 ' , to differ by less than 20 . - pubmed
Structure - activity relationship in a purine - scaffold compound series with selectivity for the endoplasmic reticulum Hsp90 paralog Grp94 . - pubmed
PCC7120 carries three genes ( all4978 , all7016 , and alr7522 ) encoding putative heme - binding GAF ( cGMP - specific phosphodiesterases , adenylyl cyclases , and FhlA ) proteins that were annotated as transcriptional regulators . - pubmed
The " as isolated " form of All4978 , identified by Soret band ( lambdamax = 427 nm ) , was assigned by electronic absorption , EPR , and resonance Raman spectroscopy as a hexa - coordinated low spin Fe ( III ) heme with a distal cysteine ligand ( absorption of delta - band around 360 nm ) . - pubmed
Cys - His ligand switches have been reported as putative signaling mechanisms in other heme - binding proteins ; however , these novel cyanobacterial proteins are the first where such a ligand - switch mechanism has been observed in a GAF domain . - pubmed
In the low - dose PEG - b - PLA - treated group , a significantly reduced number of regular estrous cycles , increased pituitary weight due to hyperemia , vascular dilatation and congestion , altered course of hypothalamic gonadotropin - releasing hormone - stimulated luteinizing hormone secretion , and increased progesterone serum levels were observed . - pubmed
Acute RV apical pacing decreased LV longitudinal strain from 16.1 + - 3.7 % in sinus rhythm to 14.4 + - 3.3 % ( p = 0.03 ) and LV base rotation from -8.4 + - 3.6 to -6.4 + - 4.0 ( p = 0.04 ) . - pubmed
Daily 2 % chlorhexidine gluconate bath wash in a tertiary adult intensive care and high dependency units to reduce risk of hospital acquired multi resistant organisms : a best practice implementation project . - pubmed
In an effort to combat increasing nosocomial infections rates within the intensive care / high dependency unit setting , Canberra Hospital has implemented a daily 2 % chlorhexidine gluconate bath wash in combination as part of a best practice policy to reduce hospital acquired multi resistant organism rates of colonization . - pubmed
METHODS : The project used the Joanna Briggs Institute 's Practical Application of Clinical Evidence System and Getting Research into Practice audit tools for promoting change in 2 % chlorhexidine gluconate wash health practice . - pubmed
CONCLUSIONS : The project was successful in increasing knowledge surrounding 2 % chlorhexidine gluconate wash administration and has provided a future direction for sustaining evidence - based practice change . - pubmed
Prophylactic measures in the form of GnRH antagonist , cabergolin and plasma expanders were given after oocyte retrieval . - pubmed
Scanning tunneling microscopy ( STM ) measurements reveal areas with reconstruction lines resulting from uniaxial strain relief due to the lattice mismatch of Fe and Ir atoms , as well as pseudomorphic strained areas . - pubmed
Magnetic field - dependent spin - polarized STM measurements of the reconstructed Fe double layer reveal cycloidal spin spirals with a period on the nm scale . - pubmed
We investigated the effects of PVA concentration ( X1 ) and solvent to antisolvent ratio ( X2 ) on the quality attributes like mean particle size ( Y1 ) ; % drug content ( Y2 ) ; and time required to 90 % drug release ( Y3 ) via 3(2 ) factorial design . - pubmed
A significant decrease in the expression of CD54 , CD80 , CD86 , and HLA - DR membrane antigens versus controls was observed after 24 hours of treatment with the different clodronate doses assayed ( P & lt ; .05 ) . - pubmed
A significant decrease ( P = .004 ) in ALP activity was found after 24 hours of treatment with the lowest dose ( 10(-9 ) mol / L ) , and a significant decrease in calcium deposition was found after 15 and 21 days of treatment ( P & lt ; .05 ) . - pubmed
Phosphatase Rtr1 Regulates Global Levels of Serine 5 RNA Polymerase II C - Terminal Domain Phosphorylation and Cotranscriptional Histone Methylation . - pubmed
Rtr1 is a conserved protein that colocalizes with RNA polymerase II ( RNAPII ) and has been shown to be important for the transition from elongation to termination during transcription by removing RNAPII CTD serine 5 phosphorylation ( Ser5 - P ) at a selection of target genes . - pubmed
Clinical Accuracy Study of an GDH - NAD Blood Glucose Monitoring System Using the Performance Criteria of ISO 15197:2013 . - pubmed
Studies have shown that the deficiency of cTnI or mutations in cTnI ( particularly in the C - terminus of cTnI ) results in diastolic dysfunction ( impaired relaxation ) due to an increased myofibril sensitivity to calcium . - pubmed
Furthermore , calcium desensitizing in correction of impaired relaxation in myocardial cells due to cTnI mutations is discussed . - pubmed
, adiponectin levels , vitamin B12 levels ) , polysomnography ( abnormalities in delta sleep ratio ) , neurochemistry ( i.e. - pubmed
Single nucleotide polymorphisms ( SNPs ) in frizzled - related protein ( FRZB ) have been associated with osteoarthritis , but their association with the risk of skeletal fluorosis has not been reported . - pubmed
While it is well established that centrally injected angiotensin II ( Ang II ) has potent actions on sympathetic nervous activity ( SNA ) , it is less clear whether peripheral Ang II can immediately stimulate SNA . - pubmed
This study shows that o - orsellinaldehyde presents anti - inflammatory and pro - apoptotic properties by acting as IKK-2 inhibitor . - pubmed
Secreted LOXL2 promotes extracellular matrix cross - linking by catalyzing the oxidative deamination of peptidyl lysine . - pubmed
We discovered that upon secretion in cell culture , LOXL2 undergoes proteolytic processing of the first two of four scavenger receptor cysteine - rich domains at the N - terminus . - pubmed
Furthermore , DEHP exposure remarkably impaired the localization and protein level of Juno , the sperm receptor on the membrane of oocytes . - pubmed
The influence of oxytocin and vasopressin on men 's judgments of social dominance and trustworthiness : An fMRI study of neutral faces . - pubmed
AVP attenuated left temporoparietal junction activity as face ratings increased , a result consistent with AVP influencing mentalization . - pubmed
Ferumoxytol and CpG oligodeoxynucleotide 2395 synergistically enhance antitumor activity of macrophages against NSCLC with EGFRL858R / T790 M mutation . - pubmed
Purpose : Drug resistance is a major challenge for epidermal growth factor receptor ( EGFR)-tyrosine kinase inhibitors ( TKIs ) treatment of lung cancer . - pubmed
Ferumoxytol ( FMT ) drives macrophage ( MPhi ) transformation towards a M1 - like phenotype and thereby inhibits tumor growth . - pubmed
CpG oligodeoxynucleotide 2395 ( CpG ) , a toll - like receptor 9 ( TLR9 ) agonist , is an effective therapeutic agent to induce anticancer immune responses . - pubmed
Results : FMT and CpG synergistically enhanced M1 - like gene expression in MPhi , including tumor necrosis factor - alpha , interleukin ( IL )- 12 , IL-1alpha , IL-1beta , IL-6 and inducible nitric oxide synthase ( iNOS ) . - pubmed
Conclusion : FMT acted synergistically with CpG to activate MPhi for suppressed proliferation and promoted apoptosis of NSCLC cells via EGFR signaling . - pubmed
Thus , combining FMT and CpG is an effective strategy for the treatment of NSCLC with EGFRL858R / T790 M mutation . - pubmed
, intragastric ) during this period attenuated the histological signs of steatohepatitis and lowered the serum and liver triglyceride contents , as well as the serum activities of aspartate aminotransferase , alanine aminotransferase , and alkaline phosphatase . - pubmed
Betulin ( 100 mg / kg ) decreased the liver / body weight ratio and inhibited the increase in the serum levels of TNFalpha , IL-1beta , TGFbeta , and hyaluronic acid , demonstrating hepatoprotective , anti - inflammatory , and antifibrotic potential . - pubmed
In addition , the expression of IDH3 significantly decreased in the carbendazim - resistant mutant nt167 ( Fgbeta2 F167Y ) . - pubmed
Furthermore , we found that carbendazim - resistance associated beta2 -tubulin substitutions reducing the interaction intensity between beta2 - tubulin and IDH3 . - pubmed
Interestingly , we demonstrated that beta2 -tubulin inhibitor carbendazim can disrupt the interaction between beta2 - tubulin and IDH3 . - pubmed
Estrogen and progesterone receptor ( ER , PR ) positivity was more common ( 47.6 % and 28.2 % , respectively ) in endometrioid carcinomas as compared with serous . - pubmed
The aim of this study was to determine the effect of DON , administered alone or with bentonite , on the histological structure of the skin and the content of collagen and elastin . - pubmed
A dose of 3.7 mg / kg DON in feed without or with 0.05 % bentonite causes the absence of immature collagen in the dermis , but the addition of 0.2 % bentonite in the feed reveals the presence of immature collagen and increase the percentage of the elastin . - pubmed
cGAS / STING - dependent apoptotic effects are required for paclitaxel response in vivo , and they are amplified by sequential , but not synchronous , administration of BH3 mimetics . - pubmed
SDA ( 50 muM ) decreased both production and cell - surface expression of intercellular adhesion molecule ( ICAM )- 1 ; however EPA and DHA resulted in greater reduction of ICAM-1 production and expression . - pubmed
EPA and DHA also significantly lowered production of monocyte chemoattractant protein 1 , interleukin ( IL )- 6 and IL-8 . - pubmed
DHA significantly decreased nuclear factor kappa - light - chain - enhancer of activated B cells ( NFkappaB)p105 gene expression and phosphorylated NFkappaBp65 protein . - pubmed
Both EPA and DHA ( 50 muM ) significantly decreased cyclooxygenase ( COX )- 2 protein . - pubmed
Nanoformulated quinacrine regulates NECTIN-4 domain specific functions in cervical cancer stem cells . - pubmed
were used to delineate the function of each domain of NECTIN-4 in cancer and their regulation by nano - formulated quinacrine ( NQC ) . - pubmed
NQC down - regulated these phenomena by modulating the endo - domain and ecto - domain of NECTIN-4 . - pubmed
Thus , current study suggested that endo - domain of NECTIN-4 translocated into nucleus and increased the DNA repair and ecto - domain of NECTIN-4 enhanced the angiogenesis , whereas NQC inhibits these processes . - pubmed
The effect of EEG on macrophage cholesterol efflux and ABCA1 / G1 expression was observed under silenced LXRalpha expression . - pubmed
RESULTS : EEG reduced serum cholesterol ( 20.33 + - 3.62 mmol / L vs 34.56 + - 8.27 mmol / L for the model group ) and LDL - C , reduced the area of arterial plaques ( 24.8 + - 10 % vs 53.9 + - 15.2 % for the model group ) and Intima / Medium thickness ratio , increased faecal bile acid content , upregulated LXRalpha , CYP7A1 , ABCA1 / G1 , ABCG5 / G8 expression in the liver , small intestine and macrophages . - pubmed
After silencing LXRalpha in macrophages , the ability of EEG to promote cholesterol efflux was inhibited . - pubmed
DISCUSSION AND CONCLUSION : EEG exert lipid - lowering and anti - atherosclerotic effects via upregulating expression of LXRalpha and downstream genes associated with reverse cholesterol transport and metabolism . - pubmed
This protocol is for isolating total RNA from either larvae ( L1 - L4 ) or adult worms using a monophasic lysis reagent . - pubmed
More importantly , chidamide was proved effective in MM by targeting SDHA , and expression of SDHA was increased by chidamide through acetylating H3K27 site of SDHA . - pubmed
The structures of the new compounds were established as 3 - O - beta - D - galactopyranosyl ( 20S , 24 R )- 3beta,16beta,25,29 - tetrahydroxy-20,24 - epoxycycloartane-29 - O - beta - D - glucopyranoside ( 1 ) , 3 - O - beta - D - glucopyranosyl -( 1 2 )- alpha - arabinopyranosyl-3beta,22xi,30 - trihydroxycycloart-24 - en-21 - oic acid alpha - L - arabinopyranosyl -( 1 6 )- beta - D - glucopyranoside ( 2 ) and 3 - O -[ alpha - L - rhamnopyranosyl -( 1 3 )- beta - D - xylopyranosyl -( 1 3 )- alpha - L - rhamnopyranosyl -( 1 2 )- alpha - L - arabinopyranosyl ]- oleanolic acid 28 - O - beta - D - glucopyranosyl ester ( 3 ) on the basis of extensive NMR and HR - ESI - MS analyses , along with acid hydrolysis . - pubmed
Besides , supplementation of PQQ Na2 within diet decreased ( P < 0.05 ) the mRNA expressions of IL-1beta and IL-10 in the duodenal mucosa of birds on day 20 . - pubmed
However , birds fed with PQQ Na2 showed higher ( P < 0.05 ) GSH - Px activity in serum and higher ( P < 0.05 ) T - SOD activities in liver on day 21 and day 42 . - pubmed
The 22 tRNA genes ranged from 65 bp ( tRNA - Cys ) to 75 bp ( tRNA - Leu1 ) . - pubmed
The chain - extension of BCP seeds by an amorphous coil - type polymer within the seed core composed of semifluorinated liquid - crystalline blocks triggers the deforming , stretching , and directional growth of the seeds along the long axis , eventually leads to nanorods . - pubmed
METHODS : six samples of PM10 , PM2.5 and PM1 were collected between the May 4th and 22nd 2020 in Madrid , on quartz fiber filters by using MCV high volume samplers ( 30 m3 h-1 flow ) with three inlets ( Digitel DHA-80 ) for sampling PM10 , PM2.5 and PM1 . - pubmed
These results suggest that the lower accumulation of glycogen in the muscle after repeated administrations of glucocorticoid may be primarily due to an abnormality in the glycogen synthetase system . - pubmed
In this particular osmotic regulation , phosphorylation of OmpR at an aspartate residue ( Asp-55 ) by EnvZ plays an important role . - pubmed
The previously isolated mutant , ompR55Q , with the amino acid replacement of Asp-55 to Gln , exhibits an OmpF- and OmpC- phenotype . - pubmed
The intragenic suppressor mutation we isolated results in the amino acid replacement of Tyr-102 to Cys in the N - terminal domain of OmpR , and exhibits an OmpF+ and OmpC+ phenotype in response to the medium osmolarity in an EnvZ - independent manner . - pubmed
It was revealed that this amino acid replacement in OmpR enhances the in vitro DNA - binding ability to the cognate DNAs . - pubmed
Responses to exogenously applied glycine and GABA were recorded using the whole - cell voltage clamp method ( pipette solution contained 121 mM - Cl- ) . - pubmed
Because glycosaminoglycans and particularly hyaluronan ( hyaluronic acid , HA ) , may play an important role in the extracellular matrix response to early injury and subsequent fibrosis , this study was undertaken to define the early time course of changes in HA and hyaluronidase . - pubmed
The specific activity of lysosomal hyaluronidase was the same in bleomycin - treated lungs and control lungs . - pubmed
The Ca channel blockers nifedipine ( 30 microM ) and verapamil ( 10 microM ) abolished the increase in Ca uptake produced by 1,25 - dihydroxyvitamin D3 . - pubmed
These results further support a direct action of 1,25 - dihydroxyvitamin D3 in the regulation of cardiac muscle Ca metabolism which may involve activation of Ca channels . - pubmed
Isoenzyme 2 was the predominant elevated isoenzyme of LDH , suggesting that the source of LDH is either hemolysis of RBCs , which occurs during freezing and thawing of bone marrow in the bags and in vivo following infusion of residual dimethyl sulfoxide . - pubmed
Beta - endorphin C - terminal peptide evokes arachidonic acid release from cortical neurones . - pubmed
Gly - Gln but not the related dipeptide , glycyl - glutamic acid , caused a 2 - fold elevation of AAM release which was blocked in the absence of extracellular calcium , in the presence of 5 mM magnesium and by the phospholipase A2 ( PLA2 ) inhibitor , mepacrine . - pubmed
The response to Gly - Gln was unaffected by D - amino-2 - phospho-5 - valeric acid ( AP5 ) and 7 - chlorokynurenic acid ( 7 - ClKY ) , antagonists respectively at the ligand and allosteric glycine binding sites of the NMDA glutamate receptor subtype . - pubmed
The results suggest that Gly - Gln causes AAM release by activating PLA2 through the mediation of a PCP / sigma - like receptor . - pubmed
Glucose reduces PDGF production and cell proliferation of cultured vascular endothelial cells . - pubmed
Both PDGF production and thymidine incorporation were significantly reduced in the endothelial cells cultured with high concentrations of glucose . - pubmed
Biochemical and thrombolytic properties of a low molecular weight form ( comprising Leu144 through Leu411 ) of recombinant single - chain urokinase - type plasminogen activator . - pubmed
rscu - PA-32k is very similar to intact recombinant single - chain urokinase - type plasminogen activator in terms of its very low activity ( 120 IU / mg ) on a chromogenic substrate for urokinase ( pyroglutamylglycylarginine p - nitroanilide ) , its plasminogen - dependent fibrinolytic activity on fibrin plates ( specific activity = 170,000 IU / mg ) , its plasminogen activating potential , and the lack of specific binding to fibrin . - pubmed
In contrast , although 0.1 mM chloroquine , an endosomal - lysosomal inhibitor , also depressed degradation ( by 57 % ) , intracellular insulin increased fourfold , indicating failure of intracellular processing . - pubmed
Furthermore , the intracellular degradative processing of insulin by these cells involves a chloroquine - sensitive pathway . - pubmed
With perfusates containing 0.83 - 1.21 mM Ca2 + ( equivalent to 8 - 11 mg / dl serum calcium ) , the number of type - I storage granules ( large core ) [ NSG - I ] and that of type - II storage granules ( small core ) [ NSG - II ] remained unchanged . - pubmed
On the contrary , with perfusates containing 1.27 mM Ca2 + ( 11.5 mg / dl ) or more , NSG - II increased and the ratio of NSG - I to NSG - II was changed reversely . - pubmed
We concluded that a threshold value of CC required for the release of storage granules may be present between 0.88 and 0.83 mM Ca2 + ( 8 and 7.5 mg / dl ) and that a threshold value of CC for accelerating the transformation of type - I granules into type - II , the degradation of storage granules , may be situated at about 1.27 mM Ca2 + ( 11.5 mg / dl ) . - pubmed
The tissue level of endogenous prostaglandins seems to exert protection since administration of cyclooxygenase inhibitors , indomethacin and aspirin , markedly increased the incidence of cecitis . - pubmed
The following agents were inactive : loperamide , an antidiarrheic agent ; carbachol , a cholinergic and diarrheogenic agent , atropine , an anticholinergic agent ; and acetazolamide , a carbonic anhydrase inhibitor . - pubmed
The amount of this extra Phe - tRNA form is greatest in slowly growing cells ( 0.8 generations / h ) , and becomes negligible in media supporting a rapid growth rate ( 2.14 generations / h ) . - pubmed
The specific enzyme activities in microsomes prepared by ultracentrifugation and by calcium aggregation were not significantly different , although the ultracentrifugation method resulted in a higher yield of hepatic microsomal protein . - pubmed
The enzyme activities in microsomes prepared by ultracentrifugation / or calcium aggregation and frozen at -20 degrees C up to 22 days were not significantly different from those of freshly prepared microsomes . - pubmed
dDAVP , a non - pressor antidiuretic analogue to ADH , induced 1 ) a high urine concentration ( 2,645 + /- 44 ( SEM ) in group E vs 131 + /- 6 mosmol / kg H2O in group C ) , P less than 0.001 ; 2 ) no significant change in plasma osmolality ( 288 + /- 2 vs 297 + /- mosmol / kg H2O respectively ) and in plasma concentration of major electrolytes , Na , K , Cl , Mg , and Ca ; 3 ) a large decrease in urinary excretion of calcium and magnesium and no change in other electrolyte or total osmolar excretion . - pubmed
Testosterone levels before and after HCG administration and LH and FSH responses to LH - RH were measured at the first visit and a few years later . - pubmed
Incubation for 48 - 72 h in culture medium supplemented with 2 mM - fructose and 0.1 microM - insulin induced the synthesis of L - type pyruvate kinase , as judged by increased enzyme activity and the increased incorporation of [ 3H ] leucine into immunoprecipitable L - type pyruvate kinase . - pubmed
The activated first component of complement ( CI ) possesses an esterase activity in vitro which will hydrolyse an ester of tyrosine to release H+ . - pubmed
Disulfide mutants of barnase . - pubmed
In this series of papers , we examine the effects of introducing disulfide bonds on the properties , structure and thermodynamics of a small globular protein , barnase . - pubmed
Two of the disulfide bonds , between residues 43 and 80 ( 43 - 80 ) and between residues 85 and 102 ( 85 - 102 ) , stabilise the protein , relative to both wild - type and the corresponding ( reduced ) dithiol forms : 85 - 102 is more stable than predicted from the entropic destabilisation of the unfolded state ; 43 - 80 is less stable than predicted . - pubmed
The third disulfide bond , between residues 70 and 92 ( 70 - 92 ) destabilises the protein relative to both wild - type and the corresponding dithiol form , implying significant disruption of the folded protein on formation of the disulfide bond . - pubmed
The disulfide bond at 43 - 80 induces small structural rearrangements close to the site of the disulfide bond , associated with some local disorder : the crosslink appears to decrease the stability of the native form of the protein . - pubmed
Inhibition of nitric oxide synthase activity in cerebral cortical synaptosomes by nitric oxide donors : evidence for feedback autoregulation . - pubmed
, NOS activity ) was found to be highly calcium - dependent and strongly inhibited ( up to 60 percent ) by NO donors , including sodium nitroprusside , hydroxylamine and nitroglycerin . - pubmed
L - Citrulline , the other major end - product from NOS , had no apparent effect on synaptosomal NOS activity . - pubmed
Taken together , these results indicate that neuronal NOS can be inhibited by NO released from exogenous donors and , therefore , may be subject to end - product feedback inhibition by NO that is formed locally within neurons or released from proximal cells . - pubmed
Investigation of the role of signal transduction in attachment of ocular melanoma cells to matrix proteins : inhibition of attachment by calmodulin antagonists including tamoxifen . - pubmed
Preferred substrates of attachment were collagens type I , III and IV and fibronectin rather than laminin , gelatin , arginine - glycine - aspartine , vitronectin , poly - L - lysine or plastic . - pubmed
Manipulation of intracellular cyclic AMP or protein kinase C activity had relatively little effect on cell attachment . - pubmed
In contrast , attachment was significantly reduced by manipulating either intracellular calcium or calmodulin . - pubmed
The experimental calmodulin antagonist J8 ( 25 microM ) , a substituted naphthalene sulphonamide , reduced attachment to 40 % . - pubmed
Similarly tamoxifen ( 25 microM ) , which has calmodulin antagonist activity in vitro , reduced attachment to 55 % . - pubmed
Both J8 and tamoxifen inhibited cell attachment to a wide range of matrix proteins , suggesting that this effect on attachment is not dependent on the presence of specific adhesion receptors . - pubmed
Reduction of ocular melanoma tumour cell / matrix interactions through manipulation of intracellular calcium or calmodulin may therefore merit further investigation as a possible approach to reducing metastatic spread . - pubmed
Bilirubin concentrations higher than 376 mumol / L for apo AI and 444 mumol / L for apo B , produce a negative interference . - pubmed
In a second approach , we have determined the capability of three toxic or metabolic injuries to induce LNGFR expression without any direct trauma of the nerve ( experimental diabetogenesis , botulinum and alpha - bungarotoxin intoxication and 2,5 - hexanedione intoxication ) . - pubmed
In a third approach , we have investigated the effect of the block of the axoplasmic transport on the LNGFR expression following different topical applications of vincristine combined with a nerve crush . - pubmed
When glucose is present , GABA caused a dose dependent inhibition of the first phase of arginine - induced insulin secretion during the range of 10 - 1000 microM , but GABA did not affect arginine - induced insulin secretion in the absence of glucose . - pubmed
GABA inhibited not only the first phase but also the second phase of glucose - induced insulin secretion . - pubmed
A GABAB - receptor agonist , baclofen , also inhibited both phases of insulin secretion induced by 16.7 mM glucose . - pubmed
These inhibitory effects of GABA on insulin secretion may be mediated through GABAB - receptor and the inhibition of the rise in cytoplasmic Ca2 + . - pubmed
In 10 mM sodium phosphate , pH 7.6 , containing 0.1 mM ethylenediaminetetraacetic acid , ions correspondings to the non - calent , four - stranded oligonucleotide , d(CGCG4GCG)4 , were detected by negative ion electrospray ionization ( ESI ) mass spectrometry at a low nozzle - skimmer ( delta NS ) bias ( -150 V ) , but not at a higher delta NS bias ( & gt ; -250 V ) . - pubmed
The postoperative course was uneventful , with complete recovery after a long period of treatment with penicillin ( IV and PO ) . - pubmed
Neither intimal nor medical SMC replication rates were decreased by GM 6001 treatment , supporting our hypothesis that the decrease in lesion size was due to inhibition of MMP - mediated migration and not inhibition of replication . - pubmed
Elevation of the markers of fibrinolysis , plasmin plasmin inhibitor complex ( PIC ) and fibrin degradation products ( FDP ) , was slower than that of TAT , peaking 7 days after treatment in both groups , the plasma PIC being significantly enhanced 7 days after treatment in the sclerotherapy group . - pubmed
The structure supports the hypothesis that the highly conserved tripeptide could play a key role in the interaction of Hsp40 with the molecular chaperone , Hsp70 . - pubmed
AML samples were studied with the MDR1 - specific MAbs MRK16 and 4E3 , and the efflux function was analyzed using Rh-123 retention in the absence or presence of verapamil . - pubmed
The MDR1 / beta 2 - M mRNA ratios for 32P - PCR were 0.41 , 0.40 and 0.12 , respectively , and were 0.127 , 0.097 and 0.028 , respectively , for QPCR . - pubmed
Peroxisomal beta - oxidation and carnitine acetyltransferase activities were increased 11- and 20 - fold , respectively , after 5 days of treatment with DHEAS ( 40 microM ) . - pubmed
Immunoblot and Northern blot analyses of acyl - CoA oxidase revealed that suppression by nicardipine of the induction of peroxisomal beta - oxidation activity would be responsible for an increase in the amount of mRNA . - pubmed
In addition , the manner in which nicardipine suppressed the induction of peroxisomal beta - oxidation and carnitine acetyltransferase activity , was similar to that of clofibric acid . - pubmed
A direct effect of CO on the activity of calcium - activated K ( KCa ) channels in vascular smooth muscle cells ( SMCs ) and the underlying mechanisms were investigated in the present study . - pubmed
Furthermore , the effect of CO on KCa channels was not mediated by cGMP or guanine nucleotide - binding proteins ( G proteins , Gi / Go or Gs ) in excised membrane patches . - pubmed
An alanine - scanning study for an NS5A / 5B substrate ( P6P4 ' ) revealed that P1 Cys and P3 Val were critical . - pubmed
Finally , substitutions at the scissile P1 Cys residue by homocysteine ( Hcy ) , S - methylcysteine ( Mcy ) , Ala , S - ethylcysteine ( Ecy ) , Thr , Met , D - Cys , Ser , and penicillamine ( Pen ) produced progressively less efficient substrates , revealing a stringent stereochemical requirement for a Cys residue at this position . - pubmed
Both ir - ACTH and ir - beta E levels increased 5 and 10 min after ether inhalation , but the increase in the ir - beta E concentration was only half that of the ir - ACTH . - pubmed
Compounds with -( CH2 ) 2- side chains terminating in basic groups such as piperidine show inhibition of telomerase at telIC50 levels of 4 - 11 microM. - pubmed
Implications for amidoanthracene-9,10 - dione telomerase inhibitors as potential anticancer agents are discussed . - pubmed
The following deductions can be made : ( 1 ) Cell injury activates enzymes : lipoxygenases generate lipid hydroperoxides ( LOOHs ) , proteases liberate Fe ions -- these two processes are prerequisites to produce radicals . - pubmed
LOHs derived from LA , hydroxyoctadecadienoic acids ( HODEs ) , surmount other markers of LPO . - pubmed
Special problems associated with the use of non - B - cell somatic cells include the processing of proinsulin to insulin , and the conferment of sensitivity to glucose - stimulated proinsulin biosynthesis and regulated insulin release . - pubmed
Regional population - specific influences have been found to affect the vitamin - D - endocrinologic - system , diminishing the influence of VDR polymorphism on BMD and on bone turnover . - pubmed
The morning following initial specimen collections , both urinary excretion rate of pyridinoline crosslinks ( Pyr ) and serum levels of bone alkaline phosphatase ( BAP ) were determined . - pubmed
The beta ( 1 )/ beta ( 2 )- chimeric receptors showed the importance of the second and seventh transmembrane domains ( TM2 and TM7 ) of the beta ( 2 ) AR for the binding of the beta(2)-selective agonists such as formoterol and procaterol . - pubmed
Alanine - substituted mutants of TM7 of the beta ( 2 ) AR showed that Tyr(308 , ) located at the top of TM7 , mainly contributed to beta ( 2 ) selectivity . - pubmed
In contrast to beta ( 2 ) AR , TM2 is a major determinant that beta(1)-selective agonists such as denopamine and T-0509 bound the beta ( 1 ) AR with high affinity . - pubmed
Three amino acids ( Leu(110 ) , Thr(117 ) , and Val(120 ) ) in TM2 of the beta ( 1 ) AR were identified as major determinants for beta(1)-selective binding of these agonists . - pubmed
Three - dimensional models built on the basis of the predicted structure of rhodopsin showed that Tyr(308 ) of the beta ( 2 ) AR covered the binding pocket formed by TM2 and TM7 from the upper side , and Thr(117 ) of the beta ( 1 ) AR located in the middle of the binding pocket to provide a hydrogen bonding for the beta(1)-selective agonists . - pubmed
Phase II / III trials testing these new agents in combination with cisplatin have been carried out in recent years with inconsistent results . - pubmed
Structural implications of the chemical modification of Cys(10 ) on actin . - pubmed
Cys(10 ) is located in subdomain 1 of actin , which has an important role in the interaction of actin with myosin- and actin - binding proteins . - pubmed
The specificity of Cys(10 ) modification was verified by showing that the 33 - kDa subtilisin fragment of actin ( residues 48 - 375 ) , which contains all of the actin thiols but Cys(10 ) , is not fluorescent . - pubmed
Cys(10 ) modification exposed a new site on actin to subtilisin cleavage . - pubmed
The modification slightly increased the rate of epsilonATP - ATP exchange and decreased the rates of G - actin ATPase and polymerization . - pubmed
The activation of S1 ATPase by Cys(10)-modified F - actin showed small probe - dependent changes in the values of V(max ) and K(M ) . - pubmed
The sliding speed of actin filaments in the in vitro motility assay remained unchanged upon modification of Cys(10 ) . - pubmed
These results indicate that although the labeling of Cys(10 ) perturbs the structure of subdomain 1 , the modified actin remains fully functional . - pubmed
The binding of S1 to actin filaments decreases the accessibility of Cys(10 ) probes to acrylamide and nitromethane quenchers . - pubmed
Because Cys(10 ) does not participate directly in either actin polymerization or S1 binding , our results indicate that actin - actin and actin - myosin interactions induce dynamic , allosteric changes in actin structure . - pubmed
No significant correlations were found between plasma vasopressin and plasma sodium or osmolality levels , but there were significant correlations between plasma vasopressin and urine osmolality , 36 ( r=0.74 , p=0 . - pubmed
Blockade of STN neuronal activity by muscimol ( GABA ( A ) receptor agonist ) injection resulted in abolishment of both the early and late excitations evoked in GP neurons by cortical stimulation . - pubmed
Injection of ( + /-)- 3 -( 2 - carboxypiperazin-4 - yl )- propyl-1 - phosphonic acid ( CPP ) [ N - methyl - D - aspartate ( NMDA ) receptor antagonist ] , but not 1,2,3 , 4 - tetrahydro-6 - nitro-2,3 - dioxo - benzo [ f ] quinoxaline-7 - sulfonamide disodium [ NBQX ( non - NMDA receptor antagonist ) ] , into the STN attenuated the early and late excitations in GP neurons , suggesting that cortico - subthalamic transmission is mediated mainly by NMDA receptors . - pubmed
Interference with the pallido - subthalamic transmission by bicuculline ( GABA ( A ) receptor antagonist ) injection into the STN made the inhibition distinct without affecting the early excitation . - pubmed
Radioligand binding assays with ( 3 ) H-1 -( 2 -( diphenylmethoxy )- ethyl )- 4 -( 3 - phenylpropyl ) piperazine ( ( 3 ) H - GBR12935 ) demonstrated that iron deficiency did not alter the affinity of the ligand for the DAT but did significantly decrease the density of the transporter by 30 % in caudate putamen and 20 % in nucleus accumbens . - pubmed
RESULTS : Loss of p53 function ( p53 - LOF ) , defined as a failure to induce p21 and/or MDM2 in response to melphalan , was seen in 1/8 drug - sensitive and 6/10 drug - resistant cell lines . - pubmed
These results suggest that desaturase genes could have , at least in part , independent control mechanisms and that Delta-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways , which may diminish the rationale for the existence of at least two distinct enzymes . - pubmed
We studied effects of haplotypes comprising 3 loci ( 5 ' to 3 ' : PvuII , HindIII , and Ser 447 -Stop ) in the lipoprotein lipase ( LPL ) gene on plasma lipid levels and LPL activity , in 807 Dutch males with coronary atherosclerosis . - pubmed
BFnon - HCO3 was 35 + /- 4 mM pH-1 for the EDL ( n = 14 ) and 86 /- 16 mM pH-1 for the soleus ( n = 14 ) . - pubmed
In soleus , 10 mM cinnamate inhibited lactate influx by 44 % and efflux by 30 % ; in EDL , it inhibited lactate influx by 37 % and efflux by 20 % . - pubmed
Cinnamate decreased [ lactate]i , in soleus by 36 % and in EDL by 45 % . - pubmed
In EDL , DIDS lowered the influx by 27 % but had almost no effect on efflux . - pubmed
DIDS reduced [ lactate]i by 20 % in soleus and by 26 % in EDL . - pubmed
However , substitution of A9 leads to relatively small changes in folding properties and cleavage activity , and the double variant G+1DAP / C25U ( DAP is 2,6 - diaminopurine ) , which could form an isosteric triple - base interaction , exhibits folding and cleavage activities that are both very impaired compared to those of the natural sequence . - pubmed
The results are interpreted in terms of an interaction between the ribose ring of C25 and the ribose and base of A10 , in agreement with the proposal of Ryder and Strobel [ Ryder , S. P. , and Strobel , S. A. - pubmed
An exception to this is provided by G8 , for which substitution with uridine leads to severe impairment of cleavage but folding characteristics that are virtually unaltered from those of the natural species . - pubmed
This is consistent with a direct role for the nucleobase of G8 in the chemistry of cleavage . - pubmed
Bradykinin ( BK ) and vascular endothelial growth factor ( VEGF )- 165 stimulate vasodilatation , microvascular permeability , and angiogenesis via the activation of the B2 - type and KDR / Flk-1 receptors . - pubmed
The two mediators induced a similar cytoskeletal reorganization and both the translocation and activation of eNOS , leading to NO release within the first minutes of cell exposure . - pubmed
At the same time , BK produced the tyrosine phosphorylation and internalization of KDR / Flk-1 as did VEGF itself . - pubmed
The selective inhibitor of VEGF receptor tyrosine kinase activity totally prevented the effects of VEGF but only partially inhibited NO release induced by BK without affecting the concomitant cytoskeletal reorganization . - pubmed
Minor effects of ACEA on Abeta - fiber - evoked responses were observed . - pubmed
Spinal pre - administration of the selective CB1 receptor antagonist SR141716A ( 0.01 microg/50 microl ) significantly reduced effects of ACEA ( 500 ng/50 microl ) on postdischarge responses of dorsal horn neurons . - pubmed
On the basis of polarity , we presumed that the unidentified lipid was a product of the lipoxygenase ( LOX ) pathway and that inclusion of the characteristic LOX inhibitors nordihydroguaiaretic acid and eicosatetraynoic acid reduced its formation in vitro and in vivo . - pubmed
Cell fractionation studies showed that the NAE amidohydrolase , responsible for FFA production , was associated mostly with microsomes , whereas LOX , responsible for NAE 18:2 - oxylipin production , was distributed in cytosol - enriched fractions and microsomes . - pubmed
The highest activity toward NAE by amidohydrolase was observed 4 to 8 h after imbibition and by LOX 8 h after imbibition . - pubmed
Investigation of pulmonary function among employees exposed to low levels of monomeric isocyanates and solvents at an automobile finishings plant . - pubmed
There have been reports in the literature of decrements in pulmonary function associated with long - term , low - level monomeric isocyanate exposure combined with solvent exposure . - pubmed
The results of the analysis demonstrated no statistically significant relationship between combined isocyanate and solvent exposure and decline in pulmonary function . - pubmed
Four of 13 patients with definite recurrence and 14 of 18 patients with no recurrence were administered azathioprine ( AZA ) as part of their post - LT therapy ( P = .01 ) . - pubmed
No significant differences were seen with donor - recipient group A , group B , group O blood type , sex , or HLA mismatches ; native liver histological characteristics ; or tacrolimus - based therapy . - pubmed
Catalase , glycolate oxidase and hydroxypyruvate reductase as well as enzymes of the fatty acid beta - oxidation pathway were demonstrated to be constituents of the microbodies in Chara indicating that they are similar to those in green leaves . - pubmed
BACKGROUND AND OBJECTIVES : Serum soluble intercellular adhesion molecule-1 ( sICAM-1 ) and total sialic acid ( TSA ) are related to the metastatic potential of cancer cells . - pubmed
The purpose of the present investigation was to determine sICAM-1 and TSA levels in colorectal carcinoma and correlate their levels with the cancer stage . - pubmed
The concentrations of sICAM-1 and TSA were measured by enzyme - linked immunoassay and the thiobarbituric acid method , respectively , and compared with those from a healthy control group ( n = 42 ) . - pubmed
CONCLUSIONS : The results of this investigation indicate that sICAM-1 and TSA are the best of the tested markers . - pubmed
Qunatitative determination of the minor hemoglobin component Hb - A2 by DEAE - cellulose chromatography . - pubmed
STUDIES OF SERUM CHOLESTEROL IN 4,244 MEN AND WOMEN : AN EPIDEMIOLOGICAL AND PATHOGENETIC INTERPRETATION . - pubmed
2NMP.3H ( 2 ) O ( Ln = Ce ( IV ) ( 1 ) , Pr ( IV ) ( 2 ) , x = 0 ; Ln = Nd ( III ) ( 3 ) , x = 1 ; NMP = N - methyl-2 - pyrrolidone ) have been prepared in aqueous solution and characterized by elemental analyses , IR , UV - vis , and TG analyses . - pubmed
Adiponectin may also be responsible for the effectiveness of thiazolidinediones , given that these drugs promote adiponectin secretion . - pubmed
Mobility of Zn , Cd and Pb in soils as affected by poultry litter extract -- II . - pubmed
After leaching , the soil samples in the columns were sequentially extracted for water soluble ( WS ) , exchangeable ( EXC ) , organic matter ( OM ) , Mn oxide ( MNO ) , amorphous Fe oxide ( AFEO ) , crystalline Fe oxide ( CFEO ) and residual ( RES ) fractions . - pubmed
The OM fraction showed high retention for Zn from the PLE . - pubmed
The EDTA redistributed Zn , Cd and Pb from the EXC , OM and MNO fractions to the WS fraction . - pubmed
Samples were analyzed for total cholesterol , triacylglycerides , high - density lipoprotein - cholesterol , and low - density lipoprotein - cholesterol . - pubmed
The production of estrogen from androgen via the estrogen biosynthesis pathway is catalyzed by aromatase P450 ( CYP19 ) . - pubmed
There was no association between CYP19 gene polymorphism and breast cancer risk in the study group as a whole , but homozygous and heterozygous carriers of the variant Arg allele showed a significantly increased risk of breast cancer among premenopausal women with a late age at first full - term pregnancy ( OR 7.31 , 95 % CI 1.88 - 28.5 ) or a high body mass index ( OR 2.77 , 95 % CI 1.12 - 6.87 ) . - pubmed
Likewise , both the ether extract and alpha - amyrin given topically dose - dependently prevented the increase of the proinflammatory cytokine interleukin-1beta levels in response to topical application of TPA . - pubmed
The exposure of VSMC to ursolic acid results in a chemotaxis inhibition , in a reduction of the expression of proliferating cell nuclear antigen ( PCNA ) and in a disorganization of beta - tubulin and vimentin cytoskeletal proteins . - pubmed
Pentachlorophenol degradation occurred in both planted and unplanted pots , but a higher rate of degradation was observed in the planted pots . - pubmed
In pure DMF , all complexes except the Zn ( II ) compounds exhibit metal - centered M(III / II ) ( Mn , Fe , Co , Ni ) or M ( II / I ) ( Cu ) redox processes . - pubmed
Fluorescence anisotropy indicated that NaCl decreased the fluidity of the external face of the plasmalemma but increased the fluidity of cell membranes in general . - pubmed
Initial isolation of whole APP was performed by immunoprecipitation , using AB10 , a monoclonal antibody raised to amino acids 1 - 17 of the beta - amyloid peptide sequence , which is present in all three alpha secretase - cleaved isoforms of interest . - pubmed
Here we will discuss data supporting the view that steroids bring about meiotic maturation through functionally redundant pathways involving synthesis of Mos or of cyclin proteins , reinforcing the robustness of the system . - pubmed
Ion trap MS / MS of intact testosterone and epitestosterone conjugates -- adducts , fragile ions and the advantages of derivatisation . - pubmed
Both compounds form multiple adducts by ESI - MS , namely protonation , ammonium and sodium , however , the mass spectrum of EG displays a more intense ammonium adduct peak than TG . - pubmed
Ammonium adduction was localised to the glucuronide ring oxygens and while EG has eight possible adduction sites , only seven were located for TG explaining the increased ammonium adduct abundance with EG . - pubmed
AG014699 and temozolomide pharmacokinetics , PARP activity , DNA strand single - strand breaks , response , and toxicity were evaluated . - pubmed
METHODS : PBC was classified as early ( pretreatment bilirubin and albumin levels normal ) , moderately advanced ( one level abnormal ) , or advanced ( both levels abnormal ) . - pubmed
Biochemical response was defined as proposed by Pares ( decrease in alkaline phosphatase [ ALP ] level>40 % of baseline level or normal level ) , Corpechot ( ALP level<3 - fold the upper limit of normal [ ULN ] , aspartate aminotransferase level&lt;2 - fold the ULN , bilirubin level<1 - fold the ULN ) , and our group ( Rotterdam ; normalization of abnormal bilirubin and/or albumin levels ) . - pubmed
Prognostic information , based on bilirubin and albumin levels , is superior to that provided by ALP levels . - pubmed
Surprisingly , loss of the Git3 glucose receptor , a G protein - coupled receptor , also increased life span in conditions where glucose consumption was not affected . - pubmed
A similar pro - aging effect of glucose was documented in mutants of hexokinase , which can not metabolize glucose and , therefore , are exposed to constitutive glucose signaling . - pubmed
Likewise , the anti - aging effect of both calorie restriction and the Deltagit3 mutation was accompanied by increased respiration and lower reactive oxygen species production . - pubmed
By immunohistochemistry , we also show that both transforming growth factor beta and the mammalian target of rapamycin are induced by constitutive activation of beta - catenin . - pubmed
In subjects in group A , glucose excursions were induced on one study day and those in group B underwent a glucose clamp ( eu- , hypo- or hyperglycaemic ) on one study day . - pubmed
CRT data were calibrated once with a retrospective calibration model based on a run - in time of 24 h and three blood glucose measurements per day . - pubmed
RESULTS : All analysable experiments , covering a broad range of blood glucose values , yielded highly accurate data for the complete experimental time with a mean relative absolute difference of 12.8 + /- 6.0 % and a predictive residual error sum of squares of 15.6 + /- 6.3 ( mean + /- SD ) . - pubmed
The CBL - CIPK Ca ( 2 +)-decoding signaling network : function and perspectives . - pubmed
The family of calcineurin B - like ( CBL ) proteins represents a unique group of calcium sensors and contributes to the decoding of calcium transients by interacting with and regulating the family of CBL - interacting protein kinases ( CIPKs ) . - pubmed
Impact of phospholipid bilayer saturation on amyloid - beta protein aggregation intermediate growth : a quartz crystal microbalance analysis . - pubmed
In the current study , a quartz crystal microbalance ( QCM ) was employed to examine the growth of Abeta(1 - 40 ) aggregation intermediates on supported phospholipid bilayers ( SPBs ) assembled at the crystal surface . - pubmed
Growth - capable Abeta ( 1 - 40 ) aggregation intermediates more readily bind SPBs composed of phospholipids with a greater degree of carbon saturation . - pubmed
CUS ( 5 - 6 - week exposure to stressors ) , but not sub - CUS ( 1 week exposure to stressors ) , induces depression - like behaviors and impairs these forms of eCB / CB1 receptor - mediated plasticity examined in the NAc core . - pubmed
However , exposure to CUS , but not to sub - CUS , attenuated the depression of fEPSPs induced by the CB1 receptor agonist WIN 55 212 - 2 . - pubmed
Thus , impaired CB1 receptor function could account for CUS - induced deficiency in eCB signaling in the NAc . - pubmed
We investigated the effects of a high TF diet on TGF - beta responsiveness in aortic endothelium and integration of cholesterol in tissues . - pubmed
Despite drastic effects on phenotype and survival , nodularin did not disturb the photosynthetic performance of plants or the structure of the photosynthetic machinery in the chloroplast thylakoid membrane . - pubmed
Moreover , the high level of cytochrome oxidase ( COX II ) , a typical marker for mitochondrial respiratory protein complexes , suggests that the respiratory capacity is increased in the leaves of nodularin - exposed plants . - pubmed
Sphingosine 1 - phosphate ( S1P ) initiates T and B cell exit from lymphoid tissues by activating the S1P ( 1 ) receptor on lymphocytes . - pubmed
Plasma - borne S1P was obviously not sufficiently inactivated by Sphingomab to account for the observed lymphopenia . - pubmed
Therefore , we addressed the local S1P - blocking activity of Sphingomab in spleen and peripheral lymph nodes ( pLNs ) as a potential cause of PBL depletion . - pubmed
In addition , exposure to rGal-1 markedly accelerated the development of apical bile canaliculi as shown by TRITC - phalloidin labeling and immunostaining for multidrug resistance associated - protein 2 ( MRP2 ) . - pubmed
Notably , rGal-1 did not interfere with multidrug resistance protein 1 / P - glycoprotein or MRP2 apical localization , neither with transfer nor secretion of 5 - chloromethylfluorescein diacetate through MRP2 . - pubmed
Stimulation of cell adhesion and polarization by rGal-1 was abrogated in the presence of thiodigalactoside , a galectin - specific sugar , suggesting the involvement of protein - carbohydrate interactions in these effects . - pubmed
Additionally , Gal-1 effects were abrogated in the presence of wortmmanin , PD98059 or H89 , suggesting involvement of phosphoinositide 3 - kinase ( PI3 K ) , mitogen - activated protein kinase and cyclic adenosine monophosphate - dependent protein kinase signaling pathways in these functions . - pubmed
The deduced amino acid sequence of AbnS1 had 70 % identity with that of Abnc . - pubmed
In contrast , expression of the abnc gene encoding Abnc was strongly induced by arabinose , arabinitol , and arabinan . - pubmed
Using debranched arabinan as substrate , recombinant AbnS1 ( rAbnS1 ) accumulated arabinobiose and arabinotriose as the major products . - pubmed
Recombinant Abnc ( rAbnc ) released mainly arabinotriose and lesser amounts of arabinose and arabinobiose than did rAbnS1 . - pubmed
Branched arabinan was completely degraded to arabinose by the action of rAbnS1 or rAbnc in combination with alpha - L : - arabinofuranosidase . - pubmed
Immune function test ( Immuknow ) is a measure of cell - mediated immunity based on peripheral CD4 + T cell adenosine triphosphate activity ( desired range , 225 - 525 ng / mL ) . - pubmed
A randomized , double - blind , phase III study of Docetaxel and Ramucirumab versus Docetaxel and placebo in the treatment of stage IV non - small - cell lung cancer after disease progression after 1 previous platinum - based therapy ( REVEL ): treatment rationale and study design . - pubmed
This article describes the treatment rationale and study - related procedures for the A Randomized , Double - Blind , Phase 3 Study of Docetaxel and Ramucirumab Versus Docetaxel and Placebo in the Treatment of Stage IV Non - Small - Cell Lung Cancer Following Disease Progression after One Prior Platinum - Based Therapy ( REVEL ) study ( I4T - MC - JVBA ; ClinicalTrials.govNCT01168973 ) . - pubmed
The successful formation of DA - stabilized copper particles was demonstrated by ultraviolet - visible spectroscopy ( UV - Vis ) , transmission electron microscopy ( TEM ) , Zeta potential measurement , and Fourier transform infrared spectroscopy ( FT - IR ) . - pubmed
Quercetin enhances the effects of 5 - fluorouracil - mediated growth inhibition and apoptosis of esophageal cancer cells by inhibiting NF - kappaB. - pubmed
Annexin V - FITC / propidium iodide ( PI)-stained fluorescence - activated cell sorter ( FACS ) analysis was used to detect the apoptotic fraction of treated cells , and western blot analysis was used to examine the protein levels . - pubmed
HEK - CaSR stimulation with PA or Ca ( 2 + ) resulted in greater pERK activation than that of stimulated HEK - WT cells . - pubmed
In addition , the DgWRKY3 transgenic plants reduced accumulation of malondialdehyde ( MDA ) and hydrogen peroxide ( H2O2 ) compared with WT plants , accompanied by higher activities of antioxidant enzymes including superoxide dismutase ( SOD ) , peroxidase ( POD ) , catalase ( CAT ) , and ascorbate peroxidase ( APX ) and the greater accumulation of antioxidants including ascorbate ( AsA ) and glutathione ( GSH ) under salt stress . - pubmed
AIM : Increased body iron is associated with insulin resistance . - pubmed
Hepcidin is the key hormone that negatively regulates iron homeostasis . - pubmed
Reduced hepcidin concentrations may cause increased body iron stores in insulin - resistant states . - pubmed
Finally induction of apoptosis by 3 - MA in CuO NP treated cells was observed by TEM . - pubmed
RESULTS : This study indicates that CuO NPs are a potent inducer of autophagy which may be a cellular defense against the CuO NP mediated toxicity and inhibition of autophagy switches the cellular response into apoptosis . - pubmed
The high concentrations of TOC , TN and BSi indicate the high nutrient level and diatom productivity in Zhelin Bay . - pubmed
Laboratory tests revealed the presence of a subgenotype A2 carrying at least seven different mutations within the S protein and high lopinavir plasma concentrations . - pubmed
The effect of thermal treatment of radiation - induced EPR signals of different polymorphic forms of trehalose . - pubmed
Electron paramagnetic resonance ( EPR ) signals induced by gamma - radiation in different polymorphic forms of trehalose were studied with dosimetry applications in view . - pubmed
Also , thermal stability and decay kinetics of the EPR signals of the different forms of trehalose were studied in isothermal annealing experiments . - pubmed
The kinetic parameters , which had been derived by fitting the Arrhenius function to the measured decay rate constants , indicated that the fading of the EPR signals varied from one polymorphic form of trehalose to another . - pubmed
Tolvaptan plus pasireotide shows enhanced efficacy in a PKD1 model . - pubmed
Compounds such as tolvaptan and pasireotide , which indirectly reduce adenylyl cyclase 6 ( AC6 ) activity , have hence proven effective in slowing cyst progression . - pubmed
Initially , the Pkd1(RC / RC ) model was inbred into the C57BL/6 background , minimizing disease variability , and the pathogenic effect of elevating cAMP was confirmed by treatment with the AC6 stimulant desmopressin . - pubmed
The river - reservoir transition zone emitted CH4 at rates an order of magnitude higher than the rest of the reservoir , and total carbon emissions ( i.e. - pubmed
FT - IR , NBO , HOMO - LUMO , MEP analysis and molecular docking study of 1 -[ 3 -( 4 - Fluorophenyl )- 5 - phenyl-4,5 - dihydro-1H - pyrazol-1 - yl ] ethanone . - pubmed
From the MEP it is evident that the negative charge covers the carbonyl group and the positive region is over the remaining groups . - pubmed
From the molecular docking studies , it is evident that the fluorine atom attached to benzene ring and ethanone attached to the pyrazoline ring are crucial for binding and the compound might exhibit inhibitory activity against TPII and may act as anti - neoplastic agent . - pubmed
Electrochemical signals are generated by differential pulse voltammetry interrogation of methylene blue ( MB ) that quantitatively binds to surface - confined hairpin probe via electrostatic interactions . - pubmed
The founding member of Ca ( 2 + ) binding proteins is calmodulin ( CaM ) , a small , acidic , modular protein endowed with gymnastic - like flexibility and E - F hand motifs that chelate Ca ( 2 + ) ions . - pubmed
In this review , I will trace the history that led to the realization that CaM serves as the Ca ( 2 +)-gating cue for SK channels , the experiments that revealed that CaM is an intrinsic subunit of SK channels , and itself a target of regulation . - pubmed
We have recently reported on the efficacy of an N - methyl - d - aspartate ( NMDA ) receptor partial antagonist , S - Methyl - N , N - diethylthiolcarbamate sulfoxide ( DETC - MeSO ) , in improving outcome following stroke , including reduced infarct size and calcium influx , suppressing the endoplasmic reticulum ( ER ) stress - induced apoptosis as well as improving behavioral outcome . - pubmed
DETC - MeSO was shown to suppress the protein kinase R - like endoplasmic reticulum kinase ( PERK ) pathway , one of the major ER stress pathways . - pubmed
Several studies including ours have provided evidence that taurine also has neuroprotective effects through reducing apoptosis and inhibiting activating transcription factor 6 ( ATF6 ) and inositol requiring enzyme 1 ( IRE-1 ) pathways . - pubmed
We hypothesized that a combined treatment with DETC - MeSO and taurine would ameliorate ischemia - induced brain injury by inhibiting all three ER stress pathways . - pubmed
Our study showed that combined DETC - MeSO and taurine , but not DETC - MeSO alone at the dose used , greatly reduced the infarct size , improved performance on the neuro - score test and attenuated proteolysis of alphaII - spectrin . - pubmed
NeuN expression levels indicated that more neurons were protected in the presence of DETC - MeSO and taurine . - pubmed
Therefore , we conclude that combined treatment with both DETC - MeSO and taurine synergistically inhibits all three ER stress pathways and apoptosis and therefore can be a novel and effective treatment after ischemic stroke . - pubmed
The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post - thaw ( P & lt ; 0.05 ) . - pubmed
The activities of superoxide dismutase , lactate dehydrogenase , glutamic - oxaloacetic transaminase and catalase improved after adding ALA to the extender ( P & lt ; 0.05 ) . - pubmed
At the highest tested concentration level ( 5000 mug L(-1 ) ) , following effects were noted : ( 1 ) inhibition on algae growth at the end of the experiment as indicated by the chlorophyll a and total biovolume ; ( 2 ) inhibition of photosynthetic efficiency on day 24 as suggested by the maximal Photosystem II quantum yield ( Fv / Fm ) ; ( 3 ) decrease of the algal diversity on day 24 and 32 as reflected by the Pielou and Shannon - Weiner indices . - pubmed
GIP response to MMT decreased to a similar extent after the 2 interventions ( p=0.977 ) . - pubmed
AIM : To investigate the relationship between serum uric acid levels and cardiovascular disease in Asians . - pubmed
Sugar phosphates were selectively captured onto the surface of the SiO2@PD - Ti ( 4 + ) microspheres by chelating with phosphate groups , and then reacted with 3 - amino-9 - ethylcarbazole via reductive amination based on solid - phase derivatization , which could not only increase the retention and resolution of sugar phosphates on reversed - phase liquid chromatography ( RPLC ) , but also improve the mass spectrometry ( MS ) sensitivity of sugar phosphates . - pubmed
Finally , the SiO2@PD - Ti ( 4 + ) microspheres combined with RPLC - MS were successfully applied to the determination of sugar phosphates from hepatocarcinoma cell lines and could even detect the trace sugar phosphates in thousands of cells . - pubmed
Colitis was induced by dextran sulfate sodium ( DSS , 2 % ) ; alpha2 - AR ligands were injected i.p . - pubmed
The nonselective alpha2 - AR agonist clonidine induced a modest aggravation of DSS - induced colitis . - pubmed
In contrast , selective inhibition of alpha2A - ARs by BRL 44408 significantly delayed the development of colitis ; reduced the colonic levels of MPO and chemokine ( C - C motif ) ligand 3 , chemokine ( C - X - C motif ) ligand 2 ( CXCL2 ) , CXCL13 , and granulocyte - colony stimulating factor ; and elevated that of tissue inhibitor of metalloproteinases-1 . - pubmed
In study 2 ( n = 106 ) , mean ( SD ) time - weighted sum of pain relief and pain intensity difference scores were 18.2 ( 20.0 ) versus 41.5 ( 21.0 ) at 0 - 12 hours and 10.3 ( 12.0 ) versus 18.4 ( 12.1 ) at 8 - 12 hours for placebo versus ibuprofen IR / ER , respectively ( P & lt ; .001 for both ) ; efficacy was sustained over each of the four 12 - hour dosing intervals with ibuprofen . - pubmed
Ibuprofen 600 mg IR / ER provided safe and effective analgesia after single and multiple doses . - pubmed
Phenylpyrrole - based HDAC inhibitors : synthesis , molecular modeling and biological studies . - pubmed
Currently , three hydroxamate - containing HDAC pan - inhibitors have been approved as antitumor agents . - pubmed
RESULTS : We herein present the development of a series of novel phenylpyrrole - based derivatives stemmed from combined computational and medicinal chemistry efforts to rationally modulate HDAC1 / 6 isoform selectivity . - pubmed
We also characterized ECP neutralizing activity using progressively truncated LPS mutants , and conclude that the polysaccharide moiety and lipid A portions are required for LPS - mediated neutralization . - pubmed
cAMP / PKA pathway appears to mediate short - term inhibitory effect of TLR2 on SERT ; however , p38 MAPK pathway has been shown to be involved in both short- and long - term TLR2 effect . - pubmed
Recognition of polyproline and a number of noncanonical sequences by SH3 domains has been extensively studied by crystallography , nuclear magnetic resonance and other methods . - pubmed
ART administration significantly decreased the average number of ACF / microscopic field . - pubmed
Similarly , LPO level was decreased and antioxidant activities were enhanced after ART treatment . - pubmed
As a result , gate - tunable photoluminescence ( PL ) effect , which is rarely achievable for MoS2 samples prepared by common CVD or mechanical exfoliation , is demonstrated . - pubmed
This study provides a simple versatile approach to fabricating monolayered crystals of MoS2 and other high - quality transition metal dichalcogenides and could lead to new optoelectronic devices based on gate - tunable PL effect . - pubmed
STEM performs the best for gold nanoparticles for all layer thicknesses , while carbon is best imaged with phase - contrast TEM for thin layers but bright field STEM is preferred for thicker layers . - pubmed
We focused on glucosylceramide synthase ( GCS ) to demonstrate that the GCS inhibitor DL - threo - PDMP resensitizes cisplatin - resistant germline - derived orthoxenografts to cisplatin . - pubmed
Even newly released anti - arrhythmic drugs , like ivabradine with HCN channel as a primary target , block the hERG potassium current in overlapping concentration interval . - pubmed
Promiscuous drug block to hERG channel may potentially lead to perturbation of the action potential duration ( APD ) and TdP , especially when with combined with polypharmacy and/or electrolyte disturbances . - pubmed
Based on this tear instability concept , the P2Y2 antagonist Diquas and the mucin secretagogue Mucosta were developed and launched in Japan over the last 5 years , and they have been widely prescribed to patients with dry eye . - pubmed
Baseline 18F - FDG PET / CT as predictor of the pathological response to neoadjuvant therapy in esophageal cancer : A retrospective study . - pubmed
Exponential - phase AOM contained a greater high - MW fraction and more carbohydrates , and the exponential cells were smaller cells and had a higher EPS content ; these characteristics resulted in a reduced average flux . - pubmed
The selected physiological parameters were plasma concentrations of thyroid hormones ( thyroxine , T4 and triiodothyronine , T3 ) , plasma proteins ( prealbumin , albumin , alpha1 - , alpha2 - , beta - and gamma - globulins ) and selected blood clinical chemical parameters ( BCCPs ) associated with liver and kidney functioning . - pubmed
Our statistical models indicated that perfluoroalkyl substances ( PFASs ) and legacy OHCs , such as polychlorinated biphenyls , organochlorinated pesticides and polybrominated diphenyl ethers , influenced only a minor fraction of the variation of plasma thyroid hormones , prealbumin and CORTf ( 5 - 15 % ) , and partly explained the selected BCCPs ( & lt;26 % ) . - pubmed
Studies must have reported baseline ( pre- ) and post - intervention ( or change of ) BMI - SDS and either fasting glucose , homeostatic model of insulin resistance ( HOMA - IR ) , alanine aminotransferase ( ALT ) , C - reactive protein ( CRP ) , or interleukin-6 ( IL-6 ) . - pubmed
From the meta - regression analyses , across all study subsets , greater mean falls in all four parameters , ( HOMA - IR , Glucose , ALT and CRP ) were observed with greater mean loss of BMI - SDS , but the trends were only statistically significant for HOMA - IR and CRP ( P=0.003 ; P=0.021 ) . - pubmed
Active oxygen species ( AOS ) play key roles in many important catalytic reactions relevant to clean energy and environment . - pubmed
BACKGROUND : Dietary Guidelines for Americans 2015 - 20 recommend choosing water in place of sugar - sweetened beverages ( SSB ) . - pubmed
OBJECTIVES : The carbohydrate - insulin model ( CIM ) predicts that increases in fasting and post - prandial insulin in response to dietary carbohydrates stimulate energy intake and lower energy expenditures , leading to positive energy balance and weight gain . - pubmed
Their fasting and post - prandial glucose and insulin levels were also measured . - pubmed
RESULTS : Only the post - prandial insulin and fasting glucose levels followed the CIM 's predictions . - pubmed
However , because higher fasting insulin was unrelated to carbohydrate intake , the overall pattern did not support the model . - pubmed
Activation of GPR120 by TUG891 ameliorated cisplatin - induced acute kidney injury via repressing ER stress and apoptosis . - pubmed
However , whether GPR120 agonism exerted potential for cisplatin - induced acute kidney injury and the involved mechanisms remained unknown . - pubmed
In our study , pharmacological activation of GPR120 by TUG891 treatment remarkably reduced the elevated serum creatinine level and attenuated tubular injury . - pubmed
Cisplatin triggered ATF6 , PERK and IRE1 pathways of unfolded protein response ( UPR ) of ER stress in the injured kidney tissue , as well as the downstream molecules eIF2alpha , ATF4 and XBP1 . - pubmed
Protein of ER stress - mediated apoptosis , CHOP , was overexpressed in the cisplatin group . - pubmed
Oral application of TUG891 displayed effective inhibition of ER stress and apoptosis . - pubmed
Collectively , activation of GPR120 by TUG891 exhibited renal protection against cisplatin - induced AKI via suppressing ER - associated apoptosis in tubular epithelial cells . - pubmed
The activity of phenoloxidase decreased significantly when dietary copper level was 86 - 162 mg / kg , and the expression of hemocyanin was up - regulated in crab fed the diets with 28 - 162 mg / kg copper . - pubmed
The main chain of AER - A3 was unambiguously determined to be smooth region and hairy region with a chain length ratio of 1:1 characterized by occurrence of ( 1 2)-alpha - Rhap , ( 1 2,3 )- alpha - Rhap , ( 1 2,4 )- alpha - Rhap , ( 1 2,3,4 )- alpha - Rhap , and ( 1 4 )- alpha - GalpA , whereas the branched chain included T - alpha - Araf , ( 1 5 )- alpha - Araf , ( 1 3,5 )- alpha - Araf , T - beta - Galp , ( 1 3 )- beta - Galp , ( 1 3,4,6 )- beta - Galp , ( 1 4 )- Glcp , ( 1 3 )- Glcp , and ( 1 3 )- Manp . - pubmed
AER - A3 had good scavenging activities against DPPH , hydroxyl , and superoxide radicals . - pubmed
Women with hypertension are more often prescribed angiotensin receptor blockers ( ARB ) , thiazide and thiazide - like diuretics . - pubmed
Insufficient prescription of medication which could improve the prognosis of the disease ( ACE inhibitors /ARB , BB , mineralocorticoid receptor antagonist ) have been identified in patients with hypertension and CAD , MI , CHF . - pubmed
Since Prxs are known to alter ROS levels by using them as electron acceptor or producing them in their catalytic activity , the impact of this overexpression in redox homeostasis was studied by analyzing the metabolites and enzymes of the ascorbate - glutathione cycle . - pubmed
In relation to the modification induced by ZePrx overexpression in lignin composition and cellular metabolism , the carbohydrate composition of the cell wall as well as overall gene expression through RNA - Seq were analyzed . - pubmed
The obtained results indicate that the overexpression of ZePrx caused an increase in syringyl lignin in cell wall stems , suggesting that ZePrx is relevant for the oxidation of sinapyl alcohol during lignin biosynthesis , coherently with its S - peroxidase nature . - pubmed
The perturbation of cellular redox homeostasis occurring as a consequence of ZePrx overexpression was kept under control by an increase in APX activity and a reduction in ascorbate redox state . - pubmed
NSCs from embryonic day 14 ( E14 ) were propagated at the presence of basic fibroblast growth factor and platelet derived growth factor alpha , and then differentiated in the medium containing triiodothyronine . - pubmed
Four extracellular matrix ( ECM ) , poly - D - lysine ( PDL ) , PDL - laminin , fibronectin , and matrigel , were examined for NSC differentiation . - pubmed
Belantamab mafodotin ( belamaf ; GSK2857916 ) is a first - in - class antibody - drug conjugate ( ADC ; or immunoconjugate ) that delivers a cytotoxic payload , monomethyl auristatin F ( MMAF ) , to myeloma cells . - pubmed
The metabotropic gamma - aminobutyric acid B receptor ( GABABR ) is a G protein - coupled receptor that mediates neuronal inhibition by the neurotransmitter GABA . - pubmed
Of the identified features , eight metabolites were significantly perturbed in the IgG4 - RD group , including glyceric acid 1,3 - biphosphate ( 1,3 - BPG ) , uridine triphosphate ( UTP ) , uridine diphosphate glucose ( UDP - Glc ) or uridine diphosphate galactose ( UDP - Gal ) , lysophospholipids , linoleic acid derivatives and ceramides . - pubmed
The interplay between a GC - rich oligonucleotide and copper ions on prion protein conformational and phase transitions . - pubmed
The prion protein ( PrP ) misfolding to its infectious form is critical to the development of prion diseases , whereby various ligands are suggested to participate , such as copper and nucleic acids ( NA ) . - pubmed
Since Cu ( II ) is a physiological ligand of PrP , we argue whether it modulates phase separation altogether with nucleic acids . - pubmed
Using recombinant PrP , we investigate the effects of Cu ( II ) ( at 6 M equivalents ) and a previously described PrP - binding CG - rich DNA ( equimolarly to protein ) on PrP conformation , oligomerization , and phase transitions using a range of biophysical techniques . - pubmed
Our results show that DNA can be an adjuvant , leading to the structural conversion of PrP , even in the presence of an endogenous ligand , copper . - pubmed
These results provide new insights into the role of Cu ( II ) and NA on the phase separation , structural conversion , and aggregation of PrP , which are critical events leading to neurodegeneration . - pubmed
Serotonylation of glutamine 5 on histone H3 ( H3Q5ser ) was recently identified as a permissive posttranslational modification that coexists with adjacent lysine 4 trimethylation ( H3K4me3 ) . - pubmed
Removal and culture of post - partum ligaments , which undergo a 75 % reduction in length and hydroxyproline content by the fifth post - partum day , allowed the demonstration of high collagenase levels released into the media . - pubmed
The rapid post - partum ligament resorption could be partially inhibited if estrogen was injected immediately following parturition , whereas progesterone or relaxin significantly impaired ligament resorption . - pubmed
In a corresponding fashion , when the collagenase levels of these ligaments were measured progesterone treatment was shown to inhibit collagenase activity markedly while estrogen was less effective . - pubmed
Other investigations for small - bowel disease may include the breath test with carbon-14 - labelled glycocholic acid , the lactose tolerance test , duodenal aspiration for giardiasis , analysis of serum immunoglobulins and , on occasion , isolation of vasoactive intestinal polypeptide hormone ( which may aid the diagnosis of functioning tumours of the pancreas or small bowel ) . - pubmed
This latter step seems to be related to the binding mode of protochlorophyllide to a holochrome protein or to conformational changes in the protochlorophyllide - holochrome . - pubmed
Modulation of acetylcholine metabolism in the hippocampal cholinergic pathway by intraseptally injected substance P. - pubmed
Local injection of substance P into the medial nuclei of the septum decreased significantly the hippocampal TRACh . - pubmed
The incorporation of ( 3H )- uridine increases to a highly significant extent in third dorsal root ganglia following NGF treatment or limb amputation . - pubmed
When NGF treatment and limb amputation were combined , no further increase in ( 3H )- uridine incorporation was noted . - pubmed
The possibility was raised that prolactin exhibits a diurnal variation in sensitivity to stress which varies as a function of circulating levels of corticosterone . - pubmed
Electrophysiological studies show that the central nervous sympathetic activity at rest and after central excitation is clearly reduced during anaesthesia with 2 vol% halothane ; 70 min after narcosis it returned to normal . - pubmed
The consensus of studies in non - insulin - dependent diabetes mellitus ( NIDDM ) seems to be that exercise has beneficial effects not only on glucose metabolism and insulin sensitivity , but also on elevated plasma lipids , blood pressure , and obesity frequently following NIDDM . - pubmed
Serum LH and testosterone levels were significantly different only in the bilaterally castrate group , compared to control levels . - pubmed
Both the normal and cryptorchid testes of all ages studied were capable of producing equal levels of testosterone in vitro , both basally and with a human chorionic gonadotropin ( hCG ) dose of 700 mIU / ml ( maximum stimulatory dose for both the normal and the 28 day cryptorchid testes ) . - pubmed
Serum FSH levels increase , but no changes occur in serum LH and testosterone levels . - pubmed
For extraocular cases we recommend color coding the thrombin with some methylene blue . - pubmed
alpha - Gal A mRNA was deficient in the mRNA preparation from the lymphoblastoid cells derived from the patient , and a faulty transcription resulting in an unstable alpha - Gal A message was suggested in this case . - pubmed
Urinary albumin excretion rates , expressed as albumin - creatinine ratios ( ACR , mg / mmol ) were measured before and after exercise in 23 claudicants and 10 controls . - pubmed
Furthermore , both tDNA and anti - tDNA are cleaved by HhaI and CfoI restriction endonucleases at two GCG / C sites which would be in double - stranded regions ( the acceptor and dihydrouridine stem ) , if the two molecules adopt the tRNA cloverleaf structure . - pubmed
In this view the 2 '- hydroxyl group , in tRNA at least , is an auxiliary structural feature whose role is limited to fostering local interactions , which increase the stability of a given conformation . - pubmed
The film contains NAD , nitro blue tetrazolium , 3 - OHB dehydrogenase , and diaphorase , and the surface is coated with modified biomembrane and can detect 50 - 1500 microM 3 - OHB within 2 - 3 min . - pubmed
The data suggest that the reduction of serum thyroxine levels after transplantation is attributable , at least in part , to inhibition of thyrotropin secretion . - pubmed
In addition to this , we calculated the count ratio of radioactivity of 99mTc - PYP ( CR ) in the infarcted myocardium to sternum to evaluate intracellular uptake of calcium during reperfusion . - pubmed
Noradrenaline caused contraction of the basilar artery , middle cerebral artery and small pial arteries by stimulation of alpha 1 - adrenoceptors . - pubmed
Ligation of fibrinogen by factor XIIIa with dithiothreitol : mechanical properties of ligated fibrinogen gels . - pubmed
Guar did not reduce the excessive postprandial glycemic excursion , glycosylated hemoglobin values , basal plasma glucose concentrations , basal or incremental plasma C - peptide values , or body weight . - pubmed
Mean low - density lipoprotein cholesterol levels were significantly reduced ( P less than .001 ) by guar administration ( 116 + /- 23 vs. 104 + /- 19 mg / dl ) . - pubmed
The present results indicate that 5 - HT mechanisms in the CNS are important mediators of the analgesic action of clonidine . - pubmed
GABA - receptor agonists that are poor substrates for GABA uptake ( muscimol , thiomuscimol , piperidine-4 - sulphonic acid , isoguvacine and 4,5,6,7 - tetrahydroisoxazolo [ 5,4 - c ] pyridine-3 - ol ( THIP ) evoked very long conductance changes that were not further prolonged by uptake inhibitors . - pubmed
Preparation of calcium phosphate for protein chromatography . - pubmed
It is concluded that the number of hippocampal corticosterone receptor sites is a sensitive index of brain aging and effectiveness of ORG 2766 . - pubmed
Systemic doses of capsaicin as low as 2.5 mg / kg depleted substance P ( SP ) in dorsal roots plus ganglia ( DRG ) whereas a 10 - mg / kg dose depleted the peptide maximally in DRG and in the dorsal spinal cord . - pubmed
High doses of capsaicin had no consistent effects on levels of cholecystokinin ( CCK ) , vasoactive intestinal polypeptide , or somatostatin , although a transient decrease in CCK levels was observed 4 days after dosing . - pubmed
In the absence of 1,25 ( OH ) 2D3 , treatment of cultures for 20 - 24 h with 10(-5 ) and 10(-6 ) M estradiol led to inhibition of 25 - hydroxyvitamin D3 [ 25 ( OH ) D3 ]- 1 - hydroxylase activity . - pubmed
When the 1 - hydroxylase was suppressed and 25 ( OH ) 2D3 - 24 - hydroxylase was induced by treatment with 1,25 ( OH ) 2D3 , estradiol in concentrations of 10(-9 ) and 10(-5 ) M either had no effect or slightly inhibited 1,25 ( OH ) 2D3 production . - pubmed
These results were unaltered when either testosterone ( 10(-6 ) M ) or insulin ( 5 micrograms / ml ) was present in the medium . - pubmed
On this basis it was suggested that the normally low concentrations of amino acids in the cerebrospinal fluid ( CSF ) , by comparison with those in plasma , were due to an active transport from the CSF to the blood . - pubmed
An H1 histamine blocker markedly diminished the skin reaction to insulin , and her plasma glucose and glycosylated hemoglobin AIc became well controlled . - pubmed
CNTF proteins with 14 amino acid residues removed from the N - terminus were biologically active whereas the removal of 23 amino acids resulted in an inactive protein . - pubmed
In addition , 18 amino acid residues could be removed from the C - terminus of the CNTF protein without apparent loss of bioactivity , but further truncation at the C - terminus yielded biologically inactive proteins . - pubmed
Brain iron , transferrin and ferritin concentrations in cytosolic and microsomal fractions of either whole brain or pons and cerebellum were then determined . - pubmed
This study extends previous research by demonstrating that the brain responds to changes in body iron status with a change in transferrin concentration . - pubmed
This study also notes that brain ferritin decreases with decreasing body iron status , though it was less responsive than nonheme iron in liver . - pubmed
Estrogen use has been reported to decrease triglyceride and low - density lipoprotein cholesterol ( LDL - C ) and increase high - density lipoprotein cholesterol ( HDL - C ) . - pubmed
In contrast to women , increased androgen concentrations in men do not seem to be associated with increased cardiovascular risk factors , although testosterone concentrations are associated with increased HDL - C and decreased insulin concentrations . - pubmed
Compared with results obtained when endotoxin was given alone , pretreatment with polymyxin B resulted in significantly ( P & lt ; 0.05 ) lower maximal plasma TNF and IL-6 activities , and significantly lower rectal temperature and respiratory rate . - pubmed
In view of the importance of cholesterol as a risk factor of cardiovascular disease , the present study analyzes the influence of the diet on blood cholesterol levels in a group of 156 teen - ager 14 to 18 . - pubmed
GF109203X , a specific PKC inhibitor , abrogates anti - CD3 antibody - induced upregulation of CD4 + T cell adhesion to B cells . - pubmed
Studies using the nonspecific inhibitor staurosporine have suggested that this phenomenon is dependent on protein kinase C activation . - pubmed
We found that the upregulation of anti - CD3 - activated CD4 + T cell adhesion was inhibited strongly and in a concentration - dependent manner by GF109203X , a compound described as a potent and selective inhibitor of PKC . - pubmed
Comparative studies showed that GF109203X and staurosporine had similar inhibitory effects on the upregulation of activated CD4 + T cell adhesion . - pubmed
PMA - activated CD4 + T cell adhesion was also inhibited by GF109203X. - pubmed
In contrast , passive enhancement of adhesion by treatment with the CD11a - specific antibody NKI - L16 was unaffected by GF109203X. - pubmed
Hemolysin B ( HlyB ) is a membrane - bound transport protein composed of an amino - terminal multiple membrane - spanning portion followed by a conserved ATP binding sequence . - pubmed
We have isolated transport - deficient mutants of HlyA altered in the C - terminal signal sequence and used one of these , a deletion of 29 amino acids , to select compensatory mutants in the transporter protein HlyB. - pubmed
Fifteen mutants located at six different sites , all mapping within the amino - terminal multiple membrane - spanning domain of HlyB , were identified . - pubmed
The diadenosine polyphosphates Ap3A and Ap4A and adenosine triphosphate interact with granulocyte - macrophage colony - stimulating factor to delay neutrophil apoptosis : implications for neutrophil : platelet interactions during inflammation . - pubmed
Adenosine triphosphate ( ATP ) and the diadenosine polyphosphates Ap4A and AP3A were almost as effective as GM - CSF in delaying neutrophil apoptosis . - pubmed
The nucleotides could thus preserve cellular morphology , protect against chromatin fragmentation , and preserve functions such as NADPH oxidase activity and expression of CD16 . - pubmed
Moreover , addition of ATP , AP3A and AP4A together with GM - CSF resulted in more pronounced protection from apoptosis than was observed during incubation with either the cytokine or the nucleotides alone . - pubmed
Because ATP , Ap3A , and AP4A may be secreted from activated platelets , these observations suggest that platelet - derived products , perhaps acting in combination with endothelial - derived or immune cell - derived cytokines , can regulate neutrophil function during certain types of inflammation . - pubmed
Use dependence of tetrodotoxin block of sodium channels : a revival of the trapped - ion mechanism . - pubmed
Nitric oxide ( NO ) , synthesized by nitric oxide synthase ( NOS ) , has negative inotropic and cytotoxic effects on cardiomyocytes . - pubmed
Expression of iNOS leads to excessive production of NO in the myocardium and may modulate cardiac contractility and ventricular morphology . - pubmed
CRT was found to be predominantly in the sera of SLE patients associated with immune complexes and C1q , and only bound to the surfaces of neutrophils in the presence of low levels of calcium and magnesium . - pubmed
In the retinal pigment epithelium , choroid plexus , adrenal gland and tongue , expression begins at E12 and in the testis and the adenohypophysis at E13 . - pubmed
In the present study , a wide range of single doses of trovafloxacin ( TR ) and twice - daily doses of ciprofloxacin ( CI ) were mimicked in an in vitro dynamic model . - pubmed
We show in this report that NPI2 , a second gene required for NH4 + -induced down - regulation of Gap1p , codes for the ubiquitin hydrolase Doa4p / Ubp4p / Ssv7p and that NH4 + -induced Gap1p ubiquitination is strongly reduced in npi2 cells . - pubmed
The npi2 mutation results in substitution of an aromatic amino acid located in a 33 - residue sequence shared by some ubiquitin hydrolases of the Ubp family . - pubmed
In proline - grown wild - type and npi2 / doa4 cells overproducing ubiquitin , Gap1p appears to be mono - ubiquitinated at two lysine acceptor sites . - pubmed
Addition of NH4 + triggers rapid poly - ubiquitination of Gap1p , the poly - ubiquitin chains being specifically formed by linkage through the lysine 63 residue of ubiquitin . - pubmed
Gap1p is thus ubiquitinated differently from the proteins targeted by ubiquitination for proteolysis by the proteasome , but in the same manner as the uracil permease , also subject to ubiquitin - dependent endocytosis . - pubmed
When poly - ubiquitination through Lys63 is blocked , the Gap1p permease still undergoes NH4 + -induced down - regulation , but to a lesser extent . - pubmed
Here we report the effects of levcromakalim ( LCRK , a channel opener ) and glibenclamide ( GBC , a blocker ) on membrane potential , whole - cell current and the cytoplasmic Ca2 + concentration of renin - secreting cells ( RSC ) . - pubmed
As monitored with the fluorescent oxonol dye DiBAC4 ( 3 ) , LCRK ( 0.3 and 1 microM ) induced a hyperpolarization of approximately 15 mV which was abolished by GBC ( 1 microM ) . - pubmed
Application of GBC ( 1 microM ) produced no effect ; however , when applied after LCRK ( 300 nM ) , GBC inhibited the opener - induced hyperpolarizing current with an IC50 of 103 + /- 36 nM. - pubmed
LCRK ( 0.3 and 1 microM ) did not significantly affect the cytoplasmic Ca2 + concentration either at rest or after stimulation by angiotensin II . - pubmed
The stimulatory effect of KATP channel opening on renin secretion is not mediated by a decrease in intracellular Ca2 + concentration . - pubmed
Kinetics of synaptotagmin responses to Ca2 + and assembly with the core SNARE complex onto membranes . - pubmed
The synaptic vesicle protein synaptotagmin I binds Ca2 + and is required for efficient neurotransmitter release . - pubmed
Here , we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2 + sensor for rapid exocytosis . - pubmed
We report that synaptotagmin is " tuned " to sense Ca2 + concentrations that trigger neuronal exocytosis . - pubmed
We further demonstrate that Ca2 + triggers penetration of synaptotagmin into membranes and simultaneously drives assembly of synaptotagmin onto the base of the ternary SNARE ( soluble N - ethylmaleimide - sensitive fusion protein [ NSF ] attachment receptor ) complex , near the transmembrane anchor of syntaxin . - pubmed
New chemotherapeutic agents , such as taxanes ( paclitaxel , docetaxel ) , topoisomerase inhibitors ( topotecan , irinotecan ) , and novel analogs ( gemcitabine , vinorelbine ) , may offer the promise of improved outcome , but have not yet been tested in phase III trials . - pubmed
Molecular therapeutics , such as gene therapy , drugs that target specific oncogene activation ( such as Ki - ras inactivation by farnesyl transferase inhibitors ) , and hypoxic cell toxins ( such as tirapazamine ) , are in clinical trials . - pubmed
The survival effect of high concentration Ang2 was blocked by pre - treatment with soluble Tie2 receptor and the PI 3 '- kinase - specific inhibitors , wortmannin and LY294002 . - pubmed
Accordingly , 800 ng / ml of Ang2 induced phosphorylation of Tie2 , the p85 subunit of phosphatidylinositol 3 '- kinase ( PI 3 '- kinase ) , and serine - threonine kinase Akt at Ser473 in the human umbilical vein endothelial cells ; lower concentrations of Ang2 ( 50 - 400 ng / ml ) did not produce notable effects . - pubmed
Ascorbic acid and D - gluconic acid lactone inhibited the hydrolysis of p - nitrophenyl beta - glucoside , but did not affect octyl - and octylthioglucoside hydrolase activity . - pubmed
Besides OTG , various other thioglucosides were hydrolysed by the novel thioglucosidase , with almost the same activities regardless of the nature of the aglycone , including the myrosinase model substrate sinigrin ( a glucosinolate ) . - pubmed
We used Brownian dynamics techniques to compute the diffusion limited association rate constants between the negatively charged phosphate ligand and several open forms of PBP ( wild - type and several mutants based on an x - ray structure of open - form PBP , mutant T141D ) . - pubmed
Oligonucleotide fishing for STAT6 : cross - talk between IL-4 and chemokines . - pubmed
Also the tyrosine - kinase inhibitor herbimycin inhibited fibrinogen mediated secretion of chemokines . - pubmed
The anxiolytic drugs diazepam and buspirone ( 1 and 5 mg / kg ) suppressed vocalization in the intertrial and SS periods . - pubmed
Anxiogenic pentyletetrazole ( 1 and 5 mg / kg ) enhanced intertrial vocalization , but did not affect its reoccurrence during the SS . - pubmed
Anxiogenic FG7142 ( 5 mg / kg ) did not affect intertrial vocalization , but blocked its reappearance on the SS . - pubmed
Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B - cell non - Hodgkin lymphomas using immunoglobulin heavy chain gene allele - specific oligonucleotide real - time quantitative - polymerase chain reaction . - pubmed
Here we demonstrate that CT also promotes the generation of regulatory T ( Tr ) cells against bystander Ag . - pubmed
Furthermore , adoptive transfer of bone marrow - derived dendritic cells ( DC ) incubated with Ag and CT induced T cells that secreted IL-4 and IL-10 and low concentrations of IL-5 . - pubmed
Acivicin - induced alterations in renal and hepatic glutathione concentrations and in gamma - glutamyltransferase activities . - pubmed
gamma - Glutamyltransferase ( gamma - GT ) catalyzes the hydrolysis of glutathione , glutathione S - conjugates , and gamma - substituted l - glutamate derivatives . - pubmed
Acivicin is an irreversible inhibitor of gamma - GT that has been used to study the role of gamma - GT in glutathione homeostasis and glutathione - dependent bioactivation reactions . - pubmed
Hence , diurnal and acivicin - induced changes in renal and hepatic glutathione concentrations along with renal and hepatic gamma - GT activities were investigated . - pubmed
Renal gamma - GT activities decreased within 2 hr after giving acivicin and remained decreased for 24 hr . - pubmed
Molecular mechanisms of TNF - alpha - induced ceramide formation in human glioma cells : P53 - mediated oxidant stress - dependent and -independent pathways . - pubmed
The present study was designed to examine the roles of p53 , reactive oxygen species ( ROS ) , and ceramide , and to determine their mutual relationships during tumor necrosis factor ( TNF )- alpha - induced apoptosis of human glioma cells . - pubmed
In cells possessing wild - type p53 , TNF - alpha stimulated ceramide formation via the activation of both neutral and acid sphingomyelinases ( SMases ) , accompanied by superoxide anion ( O2 - * ) production , and induced mitochondrial depolarization and cytochrome c release , whereas p53 - deficient cells were partially resistant to TNF - alpha and lacked O2 - * generation and neutral SMase activation . - pubmed
Restoration of functional p53 sensitized glioma cells expressing mutant p53 to TNF - alpha by accumulation of O2 - * . - pubmed
z - IETD - fmk ( benzyloxycarbonyl - Ile - Glu - Thr - Asp fluoromethyl ketone ) , but not z - DEVD - fmk ( benzyloxycarbonyl - Asp - Glu - Val - Asp fluoromethyl ketone ) , blocked TNF - alpha - induced ceramide formation through both SMases as well as O2 - * generation . - pubmed
Two separate signaling cascades , p53 - mediated ROS - dependent and -independent pathways , both of which are initiated by caspase-8 activation , thus contribute to ceramide formation in TNF - alpha - induced apoptosis of human glioma cells . - pubmed
A combination of CSF tau and MHPG discriminated PD from MSA ( adjusted odds ratios : tau , 27.2 ; MHPG , 0.14 ) . - pubmed
Ethanol inhibits palmitoylation of G protein G alpha ( s ) . - pubmed
Ethanol - induced alterations in neuronal expression of G proteins G ( s ) and G ( i ) have been invoked as a cause of heterologous desensitization . - pubmed
However , effects of ethanol on G protein expression vary considerably among different experimental protocols , various brain regions and diverse neuronal cell types . - pubmed
We studied the effect of ethanol on G alpha ( s ) palmitoylation . - pubmed
In summary , the findings identify a novel effect of ethanol on post - translational lipid modification of G alpha ( s ) , and represent a mechanism by which ethanol might affect adenylyl cyclase activity . - pubmed
Evidence indicates that both the Rho / Rho kinase signaling pathway and reactive oxygen species ( ROS ) such as superoxide and H ( 2 ) O ( 2 ) are involved in the pathogenesis of hypertension . - pubmed
The antioxidants tempol ( 3 mM ) and catalase ( 1,200 U / ml ) or the XO inhibitor allopurinol ( 400 microM ) significantly reduced X / XO - induced contraction . - pubmed
A Rho kinase inhibitor , ( + ) -( R )- trans-4 -( 1 - aminoethyl - N-4 - pyridil ) cyclohexanecarboxamide dihydrochloride ( Y-27632 ) , decreased the contraction in a concentration - dependent manner ; however , the Ca ( 2 +)-independent protein kinase C inhibitor rottlerin did not have an effect on X / XO - induced contraction . - pubmed
Phosphorylation of the myosin light chain phosphatase target subunit ( MYPT1 ) was increased by ROS , and preincubation with Y-27632 blocked this increased phosphorylation . - pubmed
These observations demonstrate that ROS - induced Ca ( 2 + ) sensitization is through activation of Rho and a subsequent increase in Rho kinase activity but not Ca ( 2 +)-independent PKC . - pubmed
CYP7B1 is the enzyme responsible for hydroxylation and termination of the estrogenic actions of the androgen metabolite , 5alpha - androstane-3beta , 17beta - diol ( 3betaAdiol ) . - pubmed
3betaAdiol is estrogenic in ERalpha or ERbeta positive cells only if they do not express CYP7B1 . - pubmed
We conclude that , by cell - specific regulation of the estrogenicity of 3betaAdiol , CYP7B1 performs two major tasks : ( i ) it allows 3betaAdiol to have growth inhibitory effects through ERbeta and ( ii ) it permits estradiol - specific activation of estrogen receptors by protection of certain cells from the estrogenic effects of 3betaAdiol . - pubmed
When CYP7B1 is inactivated , 3betaAdiol activates estrogen receptors indiscriminately , and the overall effect is prolonged and inappropriate exposure to estrogen . - pubmed
The effects of these compounds on the release of adenine nucleotides , proteins , and beta - N - acetyl - glycosaminidase ( a marker of lysosomal secretion ) from blood platelets activated by thrombin were also studied . - pubmed
Here we found that most B - cell malignancies except plasmacytoma respond to CpG oligodeoxynucleotides by up - regulating expression of costimulatory and antigen - presenting molecules , by increasing expression of CD20 , and by proliferation . - pubmed
Reactive oxygen species retain a key role during both growth factor and integrin receptor signaling , and these second messengers are recognized to be a synergistic point of confluence for anchorage - dependent growth signaling . - pubmed
Conversely , oxidation of PTKs leads to their activation , either by direct SH modification or , indirectly , by concomitant inhibition of PTPs that leads to sustained activation of PTKs . - pubmed
The GSTP1 gene displays a polymorphism at codon 105 resulting in an Ile to Val substitution , which alters the enzymatic activity of the protein , and this has been suggested as a putative high - risk genotype in various cancers . - pubmed
Enhanced post - denitrification without addition of an external carbon source in membrane bioreactors . - pubmed
This study investigates a post - denitrification process without the addition of an external carbon source combined with an enhanced biological phosphorus removal ( EBPR ) in a membrane bioreactor ( MBR ) . - pubmed
It was shown that even without dosing of an external carbon source , denitrification rates ( DNR ) much above endogenous rates could be obtained in post - denitrification systems . - pubmed
Given these surprising results , the project team decided to identify the carbon source used by the microorganisms in the post - denitrification process . - pubmed
The following hypothesis was proposed to explain the observations : the glycogen , internally stored by the substrate accumulating bacteria , if anaerobic conditions are followed by aerobic conditions could act as carbon source for denitrification in post - denitrification system . - pubmed
A single amino acid substitution in VP7 antigenic region A , at position 96 ( Asp-->Asn ) , was consistently associated with the loss of antigenic reactivity . - pubmed
While it is undisputed that estrogens ( 1 beta - estradiol , E2 ) are mainly involved in skin physiology and operate as potent hair growth modulators , our knowledge about the estrogen target cells in skin and exact signaling pathways is still very limited . - pubmed
The current review provides an overview of estrogen effects on hair follicle cycling , cutaneous expression of estrogen receptors , and potential functions of estrogens in hair biology . - pubmed
We discuss potential target genes of estrogen receptor - mediated signaling in the skin , explore the interplay of estrogens with other hormones , growth factors and enzymes , and define major open questions in this intriguing and far too long neglected area of hair research . - pubmed
Serum calcium - independent phospholipase A2 activity in bipolar affective disorder . - pubmed
However , calcium - independent PLA2 activity was significantly ( p & lt ; 0.05 ) higher in patients with a history of psychosis compared with those with no history of psychosis ( by 55 % ) or to HVs ( by 31 % ) . - pubmed
Here , we first review data evaluating the neuroprotective effects of estrogens , a selective estrogen receptor modulator ( SERM ) , and estrogen receptor alpha - and beta - selective ligands in animal models of focal and global ischemia . - pubmed
The gerbil global ischemia model was used to evaluate the neuroprotective effects of estrogen , SERMs , and ERalpha- and ERbeta - selective compounds in the hippocampus . - pubmed
Analysis of neurogranin mRNA , a marker of viability of hippocampal neurons , with in situ hybridization , revealed that estrogen treatment protected the dorsal CA1 regions not only when administered before , but also when given 1 h after occlusion . - pubmed
Region - dependent regulation of mesoaccumbens dopamine neurons in vivo by the constitutive activity of central serotonin2C receptors . - pubmed
Central serotonin2C receptors ( 5 - HT ( 2C ) Rs ) control the mesoaccumbens dopamine ( DA ) pathway . - pubmed
Intra - VTA injections of the selective 5 - HT ( 2C ) R antagonists SB 242084 and/or SB 243213 ( 0.1 - 0.5 microg/0.2 microL ) prevented the decrease in accumbal DA outflow induced by the 5 - HT ( 2C ) R agonist Ro 60 - 0175 ( 3 mg / kg , i.p ) , but did not affect the increase in DA outflow induced by the 5 - HT ( 2C ) R inverse agonist SB 206553 ( 5 mg / kg , i.p ) . - pubmed
In the perfusion model , maximal absorption and metabolism of paeoniflorin occurred in duodenum and jejunum , which were significantly decreased by a glucosidase inhibitor gluconolactone ( 20 mM ) . - pubmed
In contrast , MRP inhibitors MK-571 and leukotriene C4 did not affect transport of paeoniflorin . - pubmed
Lastly , sinomenine was also shown to significantly increase the absorptive transport of digoxin ( a prototypical p - glycoprotein substrate ) and to significantly decrease its secretory transport . - pubmed
CONCLUSIONS : Poor permeation , p - gp - mediated efflux , and hydrolysis via a glucosidase contributed to the poor bioavailability of paeoniflorin . - pubmed
Sinomenine ( an inhibitor of the p - gp - mediated digoxin efflux ) increased paeoniflorin 's bioavailability via the inhibition of p - gp - mediated paeoniflorin efflux in the intestine . - pubmed
BaP induces COX-2 expression , and an interaction between BaP and estrogen in relation to COX-2 expression is suspected . - pubmed
However , co - treatment with BaP and E(2 ) potentiated COX-2 mRNA expression and significantly elevated PGE2 secretion . - pubmed
Utilizing specific inhibitors and reporter assays , we further investigated the potentiation mechanisms of E(2 ) on BaP - induced COX-2 expression . - pubmed
First , E(2 ) activated estrogen receptor to increase PGE2 secretion , which directly increased COX-2 expression . - pubmed
Second , E(2 ) potentiated BaP - induced nuclear factor - kappaB ( NF - kappaB ) activation , which regulates COX-2 expression . - pubmed
Third , although the aryl hydrocarbon receptor ( AhR ) did not play a role in BaP - induced COX-2 expression , the potentiation effect of E(2 ) itself was AhR dependent . - pubmed
We further demonstrated that BaP induced the production of genotoxic E(2 ) metabolites ( 2 - and 4 - hydroxyestradiols ) via AhR - up - regulated cytochromes P450 1A1 and 1B1 . - pubmed
Our findings that the BaP - E ( 2 ) interaction enhanced COX-2 expression and hydroxyestradiol accumulation in the media of cultivated lung cells and tissues provide the needed scientific basis for higher risk of BaP - associated lung cancer in females . - pubmed
On the other hand , blood glucose level , serum NO , malondialdehyde ( MDA ) , a marker of lipid peroxidation , protein oxidation indices including protein carbonyl ( PCO ) and advanced oxidation protein products ( AOPP ) were significantly elevated in pancreas of the diabetic group . - pubmed
CONCLUSIONS : A significant reduction in PD occurred in two of the three groups with alpha-1 adrenergic blockers ( tamsulosin and doxazosin ) , but this effect was not sustained at 30 days . - pubmed
The polymorphisms Asn130Asp and Val174Ala in OATP1B1 and the CYP2C9 allele * 3 independently affect torsemide pharmacokinetics and pharmacodynamics . - pubmed
The IC(50 ) ( the concentration causing 50 % inhibition of cell growth ) of adriamycin ( ADR ) were assayed by MTT method , the expression of MDR1 mRNA was measured by RT - PCR , the concentration of p - glycoprotein ( P - gp ) and intracellular ADR were detected by flow cytometry . - pubmed
The following studies were performed : measurement of ( 14 ) C - labelled telavancin cellular uptake and subcellular distribution ( cell fractionation ) , determination of pericellular membrane integrity ( lactate dehydrogenase release ) , electron microscopy with morphometric analysis of changes in lysosome size and determination of total phospholipid and cholesterol content . - pubmed
Telavancin subcellular distribution was superimposable on that of a lysosomal marker ( N - acetyl - beta - hexosaminidase ) . - pubmed
It did not cause an increase in the release of lactate dehydrogenase and did not induce significant increases in total phospholipid or cholesterol content . - pubmed
Commercially available microbial biopolymers of xanthan gum and guar gum were used to encapsulate CRT wastes , reducing Pb leachability as measured by standard USEPA methods . - pubmed
Determinants of cytochrome P450 2C8 substrate binding : structures of complexes with montelukast , troglitazone , felodipine , and 9 - cis - retinoic acid . - pubmed
X - ray crystal structures of P450 2C8 complexed with montelukast ( 2.8 A ) , troglitazone ( 2.7 A ) , felodipine ( 2.3 A ) , and 9 - cis - retinoic acid ( 2.6 A ) were determined to examine ligand - protein interactions for these chemically diverse compounds . - pubmed
The structure of the 9 - cis - retinoic acid complex reveals that two substrate molecules bind simultaneously in the active site of P450 2C8 . - pubmed
In conclusion , caffeine produced useful numbers of seeds for all clones ; gliadin banding patterns and chromosome counts indicated that genetic and cytogenetic stability of the doubling process were equal or superior for caffeine compared with colchicine . - pubmed
Important aspects of bone mineral density ( BMD ) regulation are endocrine systems , notably hormonal regulation of adrenal corticoids , as indicated by clinical knowledge of glucocorticoid - induced osteoporosis . - pubmed
We studied single nucleotide polymorphisms ( SNPs ) in genes encoding thrombospondin , type 1 , domain - containing 4 and 7A ( THSD4 and THSD7A ) for possible association with lumbar and femoral BMD among 337 Japanese women with osteoporosis who participated in the BioBank Japan project . - pubmed
The transcription factor DeltaFosB accumulates in the denervated striatum and dimerizes primarily with JunD upon repeated L-3,4 - dihydroxyphenylalanine ( L - DOPA ) administration . - pubmed
Low Mannan - binding lectin serum levels are associated with complicated Crohn ' s disease and reactivity to oligomannan ( ASCA ) . - pubmed
Natural polysaccharides such as amylose ( AMY ) , alginate sodium ( ALG ) , and chitosan ( CHI ) have been noncovalently wrapped onto single - wall carbon nanotubes ( SWCNTs ) to give a series of SWCNT scaffolds , termed as AMY - SWCNT , ALG - SWCNT , CHI - SWCNT , and CHI / ALG - SWCNT scaffolds . - pubmed
Considering all properties , the relatively electrical neutral and hydrophilic AMY - SWCNT scaffolds bearing only -OH groups are able to sustain the highest cell viability after 72 h culturing . - pubmed
True catalases are tyrosine - liganded , usually tetrameric , hemoproteins with subunit sizes of approximately 55 - 84 kDa . - pubmed
paratuberculosis ( annotated as MAP-2744c ) , that retains the core elements of the catalase fold and exhibits an organic peroxide - dependent peroxidase activity . - pubmed
MAP-2744c exhibits negligible catalatic activity , weak peroxidatic activity using hydrogen peroxide ( 20 / s ) and strong peroxidase activity ( approximately 300 / s ) using organic hydroperoxides as co - substrate . - pubmed
Key amino acid differences significantly impact prosthetic group conformation and placement and confer a distinct activity to this prototypical member of a group of conserved bacterial " minicatalases " . - pubmed
Its structural features and the result of the enzyme assays support a role for MAP-2744c and its close homologues in mitigating challenge by a variety of reactive oxygen species . - pubmed
This clay was characterized according to the following techniques : Energy Dispersive Spectroscopy ( EDS ) , Thermogravimetry ( TG ) , Differential Scanning Calorimetry ( DSC ) , X - ray Diffraction ( XRD ) , Fourier Transformed Infrared Spectroscopy ( FTIR ) , Physisorption of N ( 2 ) ( BET ) , Helium Picnometry and Scanning Electron Microscope ( SEM ) with metal mapping . - pubmed
Breed differences for serum leptin , creatinine , and cholesterol were found throughout the study with AG being greater than RO . - pubmed
Although there were no breed differences ( P = 0.21 ) at TN , only AG steers exhibited a HS - induced increase ( P & lt ; 0.05 ) in prolactin , creatinine , and cholesterol concentrations to suggest that an increase in rectal temperature is required for this effect . - pubmed
The aim of our studies was to determine activity of these enzymes after induction by benzoic acids in cometabolic systems with nitrophenols . - pubmed
From cometabolic systems with nitrophenols as cometabolites and 3,4 - dihydroxybenzoate as a growth substrate , dioxygenases with the highest activity of protocatechuate 3,4 - dioxygenase were isolated . - pubmed
Catechol 2,3 - dioxygenase was active only in cultures with 4 - nitrophenol . - pubmed
Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas - chromatography coupled to a flame ionization detector ( GC - FID ) . - pubmed
The aim of this study was to develop , validate and apply a methodology based on gas chromatography coupled to optical fiber detection ( GC - OF ) for screening five fatty acids used as indicators of fungal and bacterial communities in urban soils . - pubmed
Additionally , the GC - OF showed a great potential as alternative for determination of eleven or more fatty acids in urban soils . - pubmed
DrcA is related to glutathione S - transferases , but with a key asparagine - to - cysteine substitution in the catalytic pocket . - pubmed
Mercury induces the expression of cyclooxygenase-2 and inducible nitric oxide synthase . - pubmed
Here , we report biochemical evidence that mercury alone induces NF - kappaB activation , resulting in the induced expression of COX-2 and iNOS . - pubmed
GC - MS of amaryllidaceous galanthamine - type alkaloids . - pubmed
In the present study , we focused on imipramine , a widely used antidepressant , and investigated whether it might protect against the neuronal cell death induced by tunicamycin , an ER stress inducer . - pubmed
Interestingly , in such cells application of imipramine normalized the expression of the sigma-1 receptor , which was decreased by treatment with tunicamycin alone . - pubmed
Additionally , NE-100 , a selective sigma-1 receptor antagonist , abolished the protective effect of imipramine against such tunicamycin - induced cell death . - pubmed
These findings suggest that imipramine protects against ER stress - induced hippocampal neuronal cell death both in vitro and in vivo . - pubmed
Large scale preparation of midkine antisense oligonucleotides nanoliposomes by a cross - flow injection technique combined with ultrafiltration and high - pressure extrusion procedures . - pubmed
The midkine antisense oligonucleotide ( MK - ASODN , 5'-CCC CGG GCC GCC CTT CTT CA-3 ' ) nanoliposomes have been identified to suppress hepatocellular carcinoma ( HCC ) growth effectively , and have a great potential to be an effective target drug for HCC . - pubmed
Enzymatic loss in alkaptonuria ( AKU ) , an autosomal recessive disorder , is caused by mutations in the homogentisate 1,2 dioxygenase ( HGD ) gene , which decrease or completely inactivate the function of the HGD protein to metabolize homogentisic acid ( HGA ) . - pubmed
Two new platinum ( II ) complexes , trans -[ Pt ( 2 - mpy )( 2 ) ] 4H ( 2 ) O ( 1 ) and [ PtCl ( 2 - pyc )( 2 - hmpy ) ] H ( 2 ) O ( 2 ) , where 2 - hmpy=2 -( hydroxymethyl ) pyridine , 2 - mpy = deprotonated 2 - hmpy and 2 - pyc = pyridine-2 - carboxylate , have been synthesized and characterized by elemental analysis , IR , NMR , and X - ray crystallography . - pubmed
3 - Hydroxypyridin-2 - thione as novel zinc binding group for selective histone deacetylase inhibition . - pubmed
Small molecules bearing hydroxamic acid as the zinc binding group ( ZBG ) have been the most effective histone deacetylase inhibitors ( HDACi ) to date . - pubmed
We have identified 3 - hydroxypyridin-2 - thione ( 3 - HPT ) as a novel ZBG that is compatible with HDAC inhibition . - pubmed
Remarkably , 3 - HPT gives no inhibition of HDAC 1 . - pubmed
Subsequent optimization led to several novel 3HPT - based HDACi that are selective for HDAC 6 and HDAC 8 . - pubmed
The discovery of the ribosomal origin of thiopeptides revealed a unifying theme , showing that the structural complexity arises from post - translational modifications ( PTMs ) of precursor peptides . - pubmed
Utilizing nosiheptide biosynthesis as a model system , we herein consider how nature coordinates a number of highly interwined , common and specific PTMs to accomplish the complexity of ribosomally synthesized and post - translationally modified peptides . - pubmed
After adjustment of age , sex , and BMI , FGF21 was positively correlated with total cholesterol ( P & lt ; 0.05 ) and triglyceride ( P & lt ; 0.01 ) . - pubmed
Cisplatin - induced downregulation of SOX1 increases drug resistance by activating autophagy in non - small cell lung cancer cell . - pubmed
Long - term cisplatin exposure promotes methylation of SOX1 in ovarian cancer cell , suggesting that SOX1 may be involved in cisplatin resistance . - pubmed
Our aim was to test the hypothesis that cisplatin resistance is associated with alteration of SOX1 expression in NSCLC . - pubmed
Expression of levels of SOX1 was examined using RT - PCR in cisplatin resistance cells and parental cells . - pubmed
The level of SOX1 mRNA in cisplatin resistance cells was markedly reduced when compared to parental cells . - pubmed
Promoter methylation of SOX1 was induced in cisplatin resistance cells . - pubmed
We also found that SOX1 silencing enhanced the cisplatin - mediated autophagy in NSCLC . - pubmed
The footprint periphery , including filopodia , undergoes extensive F - actin remodeling , an effect abolished by the actomyosin inhibitors cytochalasin D and blebbistatin . - pubmed
Isoproterenol induces vascular oxidative stress and endothelial dysfunction via a Gialpha - coupled beta2 - adrenoceptor signaling pathway . - pubmed
CONCLUSIONS : The beta2 - adrenoceptor / Gialpha signaling pathway is implicated in the enhanced vasoconstrictor response and eNOS uncoupling - mediated oxidative stress due to ISO treatment . - pubmed
Mlh1 - Mlh2 foci were similar to Mlh1 - Pms1 foci : they required mispair recognition by Msh2 - Msh6 , increased in response to increased mispairs or downstream defects in MMR , and formed after induction of DNA damage by phleomycin but not double - stranded breaks by I - SceI. - pubmed
No particular p53 genotype at exon 4 of codon 72 was found to be associated with oral leukoplakia , but " C " allele ( proline ) at exon 4 of codon 72 was significantly raised in these patients . - pubmed
RESULTS : Participants with pre - transition HbA1c & lt;6.5 % were older with shorter duration diabetes and took less insulin but more non - insulin glucose - lowering agents than those with higher HbA1c . - pubmed
A total of 823 participants achieved a final HbA1c & lt;6.5 % , and had greater post - transition reductions in BMI , insulin dose and secretagogue and acarbose use than those with higher HbA1c ( p & lt ; 0.0001 ) . - pubmed
HbA1c & lt;6.5 % at transition predicted final HbA1c & lt;6.5 % ( crude RR 4.9 [ 95 % CI 4.0 , 5.9 ] ; RR 3.9 [ 95 % CI 3.2 , 4.8 ] adjusted for demographics , co - interventions , pre - intervention HbA1c , BMI and glucose - lowering medication , and post - transition change in both BMI and glucose - lowering medication ) . - pubmed
By means of intracellular microelectrode recordings we found that ANG II reduced gCl in the nanomolar range and in a concentration - dependent manner ( EC50 = 0.06 muM ) meanwhile increasing potassium conductance ( gK ) . - pubmed
Both effects were inhibited by the ANG II receptors type 1 ( AT1 )- receptor antagonist losartan and the protein kinase C inhibitor chelerythrine ; no antagonism was observed with the AT2 antagonist PD123,319 . - pubmed
The scavenger of reactive oxygen species ( ROS ) N - acetyl cysteine and the NADPH - oxidase ( NOX ) inhibitor apocynin also antagonized ANG II effects on resting ionic conductances ; the ANG II - dependent gK increase was blocked by iberiotoxin , an inhibitor of calcium - activated potassium channels . - pubmed
Both ANG II and the AT1 agonist L162,313 increased the intracellular calcium transients , measured by fura-2 , with a two - step pattern . - pubmed
These latter effects were not observed in the presence of losartan and of the phospholipase C inhibitor U73122 and the in absence of extracellular calcium , disclosing a Gq - mediated calcium entry mechanism . - pubmed
The data show for the first time that the AT1 - mediated ANG II pathway , also involving NOX and ROS , directly modulates ion channels and calcium homeostasis in adult myofibers . - pubmed
Predicting scleral GP lens entrapped tear layer oxygen tensions . - pubmed
Oxygen delivery to the cornea in the presence of a contact lens system continues to be a subject of interest , and scleral GP lenses are no exception . - pubmed
This paper utilizes an existing model based on simultaneous two lens systems ( piggyback lenses ) as a resistance to oxygen in series , and applies this model to scleral GP lens systems . - pubmed
Our results may have important implications in host defense , especially the immunopotentiation function of OBP on the cyclophosphamide - induced epithelial cell injury , as well as intestinal disorders involving inflammation and infection . - pubmed
Conflicting evidence suggests that administration of allopurinol , a xanthine oxidase inhibitor can prevent CIN . - pubmed
Malondialdehyde ( MDA ) and glutathione ( GSH ) levels and catalase ( CAT ) and superoxide dismutase ( SOD ) activities in the tissues were determined . - pubmed
The activity of CAT increased only in the kidney tissue of the nebivolol+hHcy group ( P<0.01 ) , and the activity of SOD was significantly increased in all the tissues in this group . - pubmed
RESULTS : Effects of PKC activators , phorbol-12,13 - dibutyrate ( PDBu ) , and phorbol-12,13 - myristate ( PMA ) , were concentration - dependent , with high concentrations increasing frequency of micturition , and sensitivity of intramural nerves to electrical field stimulation ( EFS ) , in vitro , while lower concentrations had no effect on BMS sensitivity to EFS . - pubmed
The PKC inhibitors , bisindolylmaleimide1 ( Bim-1 ) , ( 28 nM ) , and Ro318220 ( 50 muM ) triggered an increase in the number of non - voiding contractions ( NVC ) , and a decrease in the voided volume associated with reduced ability to maintain contractile force upon EFS , but did not affect peak force in vitro . - pubmed
Application of a low concentration of PDBu inhibited spontaneous contractions , in vitro , and Bim-1 - induced NVC , and restored normal voiding frequency during urodynamic recordings in vivo . - pubmed
Tissue reactivity of both Cultrex coated and uncoated glucose sensors was evaluated at 7 , 14 , 21 and 28 days post - sensor implantation with standard histological techniques . - pubmed
Bioanalysis of emixustat ( ACU-4429 ) in whole blood collected with volumetric absorptive microsampling by LC - MS / MS . - pubmed
VC collected at birth from 156 newborns within the ALADDIN birth cohort was analyzed and 3 different groups of lipid mediators ( eicosanoids and related oxylipin analogs , endocannabinoids and sphingolipids ) were screened using LC - MS / MS . - pubmed
Somatostatin receptor ligands ( SRLs ) were the most common drug class used first line ( 57.2 % ) ; cabergoline ( 27.8 % ) was the most common treatment , followed by octreotide LAR ( 22.3 % ) and lanreotide ( 19.7 % ) . - pubmed
Investigation of selective JAK1 inhibitor GSK2586184 for the treatment of psoriasis in a randomized placebo - controlled phase IIa study . - pubmed
BACKGROUND : GSK2586184 is a selective oral Janus kinase ( JAK ) 1 inhibitor being evaluated as a treatment for moderate - to - severe plaque - type psoriasis . - pubmed
The maximum xylanase activity of optimized ( xynB - opt ) gene was 33500 U / mL after methanol induction for 144 h in 50 L bioreactor , which was 59 % higher than that by wild - type ( xynB ) gene . - pubmed
Effects of academic exam stress on nasal leukotriene B4 and vascular endothelial growth factor in asthma and health . - pubmed
We determined LTB4 and VEGF concentrations using Enzyme - Linked Immunoassays . - pubmed
Changes in nasal LTB4 and VEGF were not associated with salivary cortisol , exhaled nitric oxide , or spirometric lung function . - pubmed
Corticosterone , insulin and glucose concentrations all increased with temperature , but with different thermal response curves , suggesting that high temperatures differently affect energy - regulation pathways . - pubmed
Involvement of the NADPH Oxidase NOX2 - Derived Brain Oxidative Stress in an Unusual Fatal Case of Cocaine - Related Neurotoxicity Associated With Excited Delirium Syndrome . - pubmed
Here , we investigated the possible role of the Nicotinamide Adenine Dinucleotide Phosphate oxidase NOX2 - derived brain oxidative stress in a fatal case of cocaine - related neurotoxicity , associated to excited delirium syndrome . - pubmed
These results support a crucial role of NOX2 - derived brain oxidative stress in cocaine - induced brain dysfunctions and neurotoxicity . - pubmed
A binary logistic regression analysis was conducted to predict the presence or absence of MAT in the treatment plan of pregnant women using the following independent variables : state prenatal child abuse law , referral source , geographical region , and Medicaid coverage of methadone . - pubmed
Conversion of vinyl pyranosylamine and vinyl furanosylamines to 2,6 - and 2,5 - disubstituted pyrrolidine and piperidine iminosugars , respectively , in one pot was developed using Kimura and Tamaru 's procedure , where a Pd salt in the presence of Et2Zn was used for the domino reaction . - pubmed
Photosynthesis - nitrogen relations in Amazonian tree species : II . - pubmed
Mothers / caregivers visiting health facilities obtained mainly oral rehydration salt ( ORS ) ( 39.8 % ) and home - recommended fluids ( HRF ) ( 40.3 % ) , but few of them ( 11.9 % ) obtained ORS plus zinc supplementation . - pubmed
Symptomatic responses did not differ significantly between regimens ; both gave clavulanate levels sufficient to inhibit beta - lactamase activity . - pubmed
Different treatment approaches were observed with corticosteroids administered in 87 % , cyclosporine in 49 % , etoposide ( VP-16 ) in 39 % , and monoclonal anti - TNF in 6 % of cases . - pubmed
Interestingly we observed differences in the role of molecules involved in damage sensing ( TOLL - like receptors ) , damage signalling ( MyD88 ) and immune regulation ( cytokines , CD73 , lymphocytes ) for the pathogenesis and progression of DNA damage - induced pneumopathy between the models of pneumopathy induced by whole thorax irradiation or treatment with the radiomimetic drug BLM . - pubmed
While miltefosine inhibits the synthesis of phosphatidylcholine and also affects the parasite mitochondrion , inhibiting the cytochrome c oxidase , it is to be expected that this potent drug also produces its effect through other targets . - pubmed
Grade IV - titanium threaded implants were placed at 4 weeks post - extraction in maxillary first molars . - pubmed
For instance , in the control group of the fluid - mechanical model , MRI - TIFP was a strong predictor of WIN - TIFP ( R2 = 0.76 , p & lt ; .0001 ) . - pubmed
A similar result was found in the bevacizumab - treated group of the empirical model ( R2 = 0.93 , p = .014 ) . - pubmed
CNO treatment induced pro - inflammatory mediator production per se from cells expressing Gq - DREADDs , and inhibited lipopolysaccharide- and CCL2 - induced inflammatory signaling from cells expressing Gi - DREADDs . - pubmed
Protective role of rosmarinic acid on amyloid beta 42 - induced echoic memory decline : Implication of oxidative stress and cholinergic impairment . - pubmed
In the present study , we examined whether rosmarinic acid ( RA ) reverses amyloid beta ( Abeta ) induced reductions in antioxidant defense , lipid peroxidation , cholinergic damage as well as the central auditory deficits . - pubmed
Abeta42 injection led to a significant increase in the levels of thiobarbituric acid reactive substances ( TBARS ) and 4 - Hydroxy-2 - nonenal ( 4 - HNE ) but decreased the activity of antioxidant enzymes ( SOD , CAT , GSH - Px ) and glutathione levels . - pubmed
Moreover , Abeta42 injection resulted in a reduction in the acetylcholine content and acetylcholine esterase activity . - pubmed
The existence of a so - called brain renin - angiotensin system ( RAS ) is controversial . - pubmed
Given the presence of the blood - brain barrier , angiotensin generation in the brain , if occurring , should depend on local synthesis of renin and angiotensinogen . - pubmed
RESULTS : When methanol fixation protocol was broken up into three steps : fixation , storage and rehydration , we found that PBMC RNA was degraded during rehydration with PBS , not at cell fixation and up to 3 - month storage steps . - pubmed
Resuspension but not rehydration in 3x saline sodium citrate ( SSC ) buffer instead of PBS preserved PBMC RNA integrity and prevented RNA leakage . - pubmed
Lead Optimization of 5 - Aryl Benzimidazolone- and Oxindole - Based AMPA Receptor Modulators Selective for TARP gamma-8 . - pubmed
Herein , we describe the discovery , lead optimization , and preclinical characterization of 5 - arylbenzimidazolone and oxindole - based negative modulators of AMPARs associated with TARP gamma-8 , the primary TARP found in hippocampus . - pubmed
High - throughput screen lead 4 was optimized for potency and brain penetration to provide benzimidazolone 3 , JNJ-55511118.1 Replacement of the benzimidazolone core in 3 with an oxindole mitigated reactive metabolite formation and led to the identification of 18 ( GluA1 / gamma-8 pIC50 = 9.7 ) . - pubmed
Urea was shown to promote the deregulation of STL1 expression , overcoming glucose repression . - pubmed
The consistency between Fps1 - mediated ethanol secretion and ethanol in the extracellular media reinforces previous suggestions that ethanol might exit the cell through the Fps1 channel . - pubmed
The hormone - sensitive lipase ( HSL ) and Ser563 - pHSL levels were also significantly reduced by treatment with 5 mg / mL of ABG extract . - pubmed
Upon incorporation of the additives we achieve space - charge limited current characteristics that resemble molecular single crystals such as rubrene with high , trap - free SCLC mobilities up to 0.2 cm2 / Vs and a width of the residual tail state distribution comparable to kBT . - pubmed
Both theoretical prediction and experimental photophysical data indicate a small S1 - T1 energy gap ( DeltaE ST ) and a relative large fluorescence rate ( k F ) in an o - phenylene - bridged 2 - cyanopyrazine/3,6 - di - tert - butylcarbazole compound ( TCzPZCN ) . - pubmed
A sky - blue device containing a derivative of TCzPZCN achieves an improved EQE maximum of 12.2 % by suppressing the non - radiative decay at T1 . - pubmed
Can carbene decorated [ FeFe ]- hydrogenase model complexes catalytically produce dihydrogen ? - pubmed
Cyclic alkyl amino carbene ( CAAC ) anchored [ FeFe]-hydrogenase model complex featuring rotated conformation at one of the iron centers are found to be promising candidate for effective production of dihydrogen . - pubmed
Our results indicated that after knockdown of NF - kappaB , the autophagy was significantly increased and the expression of p - AKT and p - mTOR protein markedly decreased , but the level of autophagy was inhibited after treatment with AKT activator SC79 , suggesting the involvement of AKT / mTOR - mediated autophagy was under autophagy activation . - pubmed
As for co - AD of two feedstocks , introduction of SS ( 25 % , VS basis ) in FW significantly improved the process stability and archaea / total microbe ratio ( from 0.4 % to 17.1 % ) , which might be due to the regulating effect of abundant trace metals in SS ; co - AD of SS ( 25 % , VS basis ) with YW improved the methane yield by 2.04 times than AD of YW only together with higher methane contents ( 57.4 + - 1.3 % vs. 50.9 + - 2.2 % ) ; in co - AD of FW and YW , synergistic effects in terms of increased methane production ( 3.4 - 19.1 % ) were observed , which was correlated with more robust growth of both bacteria and archaea . - pubmed
Resection of the thymoma treated her myasthenia gravis , but led to an aplastic anaemia resistant to granulocyte - colony stimulating factor , cyclosporin and horse anti - thymocyte globulin treatment . - pubmed
Herein , a multi - arm star - branched polymer was prepared by conjugating oligolysine onto a rigid hyperbranched poly ( amido amine ) core via click chemistry , followed by covalent modification with Gd chelate and folic acid ( FA - OLL - g - HBPAMAM - DTPA - Gd ) . - pubmed
This multi - arm star - branched polymer contrast agent exhibited much higher longitudinal relaxivity ( r1 = 13.34 mM-1 s-1 ) as compared to a commercial contrast agent ( Gd - DTPA , r1 = 4.2 mM-1 s-1 ) . - pubmed
The study results indicated that compared to PDS , PRP - loading PDS exhibited the enhanced mechanical properties and released growth factors in a slow and sustained manner to supplement the loss of growth factors during decellularization . - pubmed
When compared with PDS , PRP - loading PDS significantly promoted the colonization , proliferation and pro - angiogenic genes expression of cells on scaffolds . - pubmed
RESULTS : The central administration of galanin enhanced 2 - deoxy -[ 3H ]- D - glucose , peroxisome proliferator - activated receptor gamma and adiponectin levels , food intake and body weight , GLUT4 mRNA expression and GLUT4 concentration in plasma membranes , as well as homeostasis model assessment - insulin resistance index . - pubmed
Those effects of galanin may be blocked by M817 , and imitated by M1145 except for food intake and body weight . - pubmed
Stomatal closure induced by darkness or ABA was inhibited by the H2O2 - scavenging enzyme catalase or the antioxidant N - acetyl cysteine ( NAC ) , or by diphenylene iodonium ( DPI ) , an inhibitor of the H2O2 - generating enzyme NADPH oxidase . - pubmed
Such increases were inhibited by catalase , NAC or DPI , consistent with the effects of these compounds on stomatal apertures . - pubmed
The bladders were dissected for the tissue antioxidant enzyme activities and lipid peroxidation , including malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) , total antioxidant status ( TAS ) and total oxidant status ( TOS ) . - pubmed
Moreover , we reveal that this H7N9 protein prevents the interaction between TRAF3 and cIAP2 that mediates Lys48 - linked polyubiquitination of TRAF3 for degradation . - pubmed
During photosynthesis , an enzyme Ribulose 1,5 bisphosphate carboxylase / oxygenase ( Rubisco ) combines its substrate Ribulose 1,5 bisphosphate ( RuBP ) with CO2 to produce phosphoglycerate ( PGA ) . - pubmed
Alongside , Rubisco also takes up O2 and produce 2 - phosphoglycolate ( 2 - PG ) , a toxic compound broken down into PGA through photorespiration . - pubmed
The C3 model at elevated level of inorganic phosphate ( Pi ) , exhibited a significant change in RuBP ( + 355 % ) and PGA ( - 98 % ) which is attributable to the low availability of Ci . - pubmed
Post - synthetic ' click ' synthesis of RAFT polymers with pendant self - immolative triazoles . - pubmed
We investigate the polymerization kinetics , post - synthetic modification , and self - immolation mechanism of a model polymer system , and discuss avenues for future studies on poly - pendant self - immolative triazoles as a modular , stimuli - responsive macromolecule platform . - pubmed
In the presence of an ATPase ( adenosinetriphosphatase ; ATP phosphohydrolase ; EC 3.6.1.3 ) inhibitor dicyclohexylcarbodiimide , pHin remained equal to pHex even in the presence of glucose . - pubmed
Ethanol - induced inhibition of guanylate cyclase in liver , pancreas , stomach and intestine . - pubmed
Ethanol decreases hepatic protein and albumin synthesis , and inhibits pancreatic water , bicarbonate , and protein secretion . - pubmed
Since these actions of ethanol are opposite to those reported for secretin , cholecystokinin - pancreozymin , and pentagastrin which may be mediated through increases in cyclic GMP , it appeared possible that the inhibitory actions of ethanol might be mediated through inhibition of guanylate cyclase , the enzyme that catalyzes the production of cyclic GMP . - pubmed
The inhibitory effects of ethanol on the guanylate cyclase - cyclic GMP system of these tissues provide a possible explanation for some of the diverse effects of ethanol on these tissues . - pubmed
Markers of this group controlling the regulation of riboflavin biosynthesis were mapped between markers of resistance to acriflavin and streptomycin ( strC group ) . - pubmed
The value of cotransfer index between acriflavin - resistance markers and ribC markers was found to be 26 - -32 % . - pubmed
Three subgroups were identified : One was unrease - positive , produced porphyrin from delta - aminolevulinic acid , and grew on infusion mediums supplemented only with V factor ; the 2nd was unrease - negative , porphyrin - positive , and grew only on serum - enriched mediums with added V factor ; and the 3rd was unrease - negative , porphyrin - negative , and grew only on serum - enriched mediums with added V and X factors . - pubmed
Other tests , such as beta - galactosidase , hemolysis , and fermentation of carbohydrates were of secondary value in differentiating between these species . - pubmed
The core peptide derived from cyanogen bromide cleavage have been further digested with trypsin and the resulting peptides have been separated on Dowex 1X2 . - pubmed
All fragments have been characterized by their amino acid composition , by determination of their N - terminal sequence using automatic Edman degradation and of their C - terminal sequence following the kinetics of amino acid cleavage by carboxypeptidases A and B. - pubmed
In contrast to guanethidine - treated parotid glands , no retention of alpha - amylase was observed . - pubmed
Level of plasma prekallikrein and its inhibitors in reactors and nonreactors during intravenous enhancement with contrast media . - pubmed
No effect was seen on plasma triglycerides , cholesterol , HDL - cholesterol and gamma - glutamyltranspeptidase at any stage of the trial . - pubmed
No other plasma free essential fatty acid changed during the trial , although the HDL : cholesterol increased slightly but non - significantly with an increase in EPA and DHA . - pubmed
Upon sperm incubation in the presence of free insulin , a dose - dependent release of phospholipid phosphorus occurred , with a concomitant derangement of head cell membrane . - pubmed
Analyses of the levels of HMGr and LDLr mRNAs under a variety of culture conditions that perturb intracellular sterol metabolism , or which differ in the levels of extracellular sterols , indicated that the hepatoma cells and fibroblasts responded similarly in terms of the repression or induction ratios of both mRNAs . - pubmed
A ruthenium complex ( ICR ) had a higher activity in the resistant line . - pubmed
In the presence of extracellular Ca2 + ( CaDT - PA added ) , the rise in [ Ca2 +] i was due to a combination of Ca2 + release from internal stores and influx of extracellular Ca2 + . - pubmed
In the absence of extracellular Ca2 + , LXA4 dose - dependently reduced the subsequent response of neutrophils to fmlp ; this is presumably because LXA4 discharges the store , and so reduces the amount of Ca2 + available for subsequent release by fmlp . - pubmed
Three of these peptides , vasoactive intestinal peptide , substance P , and somatostatin , regulate the function of immune effector cells in gut - associated lymphoid tissue . - pubmed
Vasoactive intestinal peptide modulates lymphocyte migration and natural killer cell activity by a cyclic adenosine monophosphate ( cAMP)-dependent mechanism , whereas substance P induces mediator release by a cAMP - independent mechanism . - pubmed
Somatostatin antagonizes the effects of both vasoactive intestinal peptide and substance P by a mechanism that appears to involve inhibitory guanine nucleotide binding proteins . - pubmed
We describe here a limiting dilution analysis to determine cytotoxic T lymphocyte precursor ( CTLp ) frequencies against individual HLA - A or -B antigens . - pubmed
This LDA is a practicable tool for a systematic analysis of CTLp response against selected individual HLA - A or -B antigens and can be used for the selection of HLA mismatched donors for transplantation patients . - pubmed
( + /-)- Cyanopindolol increased the evoked 3H overflow ; this effect was prevented by preexposure to the previously characterized serotonin receptor antagonist metitepin . - pubmed
The present results show that the presynaptic serotonin autoreceptor is blocked by cyanopindolol in a stereoselective way . - pubmed
This drug is 20 times more potent than metitepin as an antagonist at the presynaptic serotonin autoreceptor , and , in contrast to the latter , it does not act as an antagonist at the presynaptic alpha 2 - adrenoceptor on the serotoninergic neurone . - pubmed
A possibility of increasing the survival of cells by the pre- and post - irradiation treatment with a mesoporphyrin - IX derivative was demonstrated . - pubmed
A single exon encodes the carboxyl - terminal 26 amino acids of the ssd chain and the 3 ' untranslated region of its mRNA , ending with a poly(A)-addition site . - pubmed
Normal serum and CSF chloride concentrations were restored . - pubmed
Calculations made on the basis of rate of CSF production , CSF chloride concentration , and duration of anorexia provided supportive evidence for an active transport system for chloride from plasma to CSF . - pubmed
In contrast , hepatic cholesteryl ester levels , rates of hepatic LDL transport , and concentrations of plasma LDL - cholesterol were not affected by bile acid feeding in this species . - pubmed
Ursodeoxycholic acid , in contrast , does not affect these processes either directly or indirectly and so causes no change in plasma LDL levels . - pubmed
The carbohydrate moiety of human platelet glycocalicin : the structures of the major Asn - linked sugar chains . - pubmed
The released carbohydrate chains were found to be of the complex - type from their interaction with immobilized lectin columns . - pubmed
The structures of the two major oligosaccharide - alditols separated by ion - exchange chromatography on a Mono Q column were investigated by means of methylation analysis , glycosidase digestion , and Smith periodate degradation , and they were assigned as typical di- and trisialylated complex - type oligosaccharide - alditols with two and three peripheral chains consisting of Gal - GlcNAc sequences , respectively . - pubmed
Results demonstrated that neonatal hearts had a significantly shorter ( p less than .05 ) TIC ( 29.5 + /- 1.7 min ) than adult hearts ( 43.0 + /- 2.9 min ) and exhibited more rapid lactate accumulation ( 2.5 + /- 0.5 to 22.1 + /- 3.0 , p less than .001 ) and an increase in G-6 - P ( 0.07 + /- 0.03 to 0.34 + /- 0.07 , p less than .01 ) with a nonsignificant decline in myocardial glycogen ( 23.3 + /- 12.1 to 17.4 + /- 4.0 , p greater than .05 ) . - pubmed
The lysosomal degradation of ganglioside GM2 by hexosaminidase A depends on the presence of the specific activator protein which mediates the interaction between micellar or membrane - bound ganglioside and water - soluble hydrolase . - pubmed
In the absence of enzyme the activator acts in vitro as glycolipid transfer protein , transporting glycolipids from donor to acceptor membranes . - pubmed
Three duodenal juice samples ( at 30 , 60 and 90 mins of hormonal infusion ) were taken in each subject for CEA , cytology , bicarbonate and trypsin determinations . - pubmed
Sequential treatment of gelatinase A with 4 - aminophenylmercuric acetate ( APMA ) and neutrophil elastase yielded an active gelatinase with a 4 - fold increase in gelatinolytic activity . - pubmed
In the presence of gelatin , these two inactivating cleavage sites were protected , and cleavage at a site within the hemopexin - like carboxyl - terminal domain resulted in a truncated yet active gelatinase . - pubmed
We assessed the efficacy and safety of the lipid - lowering drugs , nicotinic acid ( short acting ) and lovastatin , the 3 - hydroxy-3 - methylglutaryl coenzyme A reductase inhibitor . - pubmed
At 16 weeks , when compared with control values , nicotinic acid ( & gt ; or = 1.5 g twice a day ) significantly reduced the total cholesterol ( from 312 + /- 18 [ + /- SEM ] mg / dL to 229 + /- 19 mg / dL ; P = 0.03 ) and the low - density lipoprotein cholesterol ( from 218 + /- 15 mg / dL to 142 + /- 13 mg / dL ; P = 0.03 ) and significantly increased the high - density lipoprotein cholesterol ( from 44 + /- 3 mg / dL to 58 + /- 5 mg / dL ; P = 0.03 ) . - pubmed
At 16 weeks , lovastatin therapy ( 40 mg / d ) significantly reduced the total cholesterol ( from 285 + /- 13 mg / dL to 233 + /- 10 mg / dL ; P = 0.005 ) and the low - density lipoprotein cholesterol ( from 201 + /- 11 mg / dL to 147 + /- 7 mg / dL ; P = 0.001 ) . - pubmed
There were no significant changes in the triglyceride and high - density lipoprotein cholesterol levels . - pubmed
Whole blood samples were collected in EDTA and assayed using a commercially available radioimmunoassay kit that employs a monoclonal antibody specific for Cy . - pubmed
Can tests for IgA , IgG , or IgM antibodies to cardiolipin or phosphatidylserine substitute for lupus anticoagulant assays in screening for antiphospholipid antibodies ? - pubmed
We evaluated ELISA tests for IgG , IgM , and IgA isotopes of antibodies binding cardiolipin ( CL ) and phosphatidylserine ( PS ) in samples from LA patients presenting with recurrent miscarriages . - pubmed
Neither the use of PS as the antigen nor the addition of IgA assays improved the correlation of ELISA results with the presence of LA . - pubmed
TNF - alpha - induced adherence to fibronectin was suppressed from 69 % + /- 5 % of the phorbol myristate acetate response to 38 % + /- 7 % ( P = 0.0154 ) . - pubmed
The androgen receptor ( AR ) gene contains a polymorphic CAG microsatellite that codes for a variable length of glutamine repeats in the AR protein . - pubmed
The deduced amino acid sequences of the alpha , beta 2 , beta 3 , beta 4 and gamma isoforms predict molecular weights of 57,600 , 56,500 , 60,900 , 52,500 , and 68,000 , respectively . - pubmed
Thus both gonadotropins contribute , directly for LH and indirectly , through paracrine mecanisms , for FSH , to testosterone production . - pubmed
Concomitantly , cocaine prevented the maximal producible HTR frequency via simultaneous indirect stimulation of the inhibitory presynaptic 5 - HT1A and postsynaptic alpha 2 receptors . - pubmed
In the present study , we have investigated the effects of cocaine and the selective 5 - HT ( sertraline ) , norepinephrine ( nisoxetine ) and dopamine ( GBR 12935 ) uptake inhibitors on the L - tryptophan - induced HTR in the presence of a nonselective monoamine oxidase inhibitor , tranylcypromine . - pubmed
Cocaine potentiated the ability of L - tryptophan to induce HTR to a greater extent in protocol 1 , whereas sertraline induced a greater effect in protocol 2 . - pubmed
However , in our earlier study cocaine ( and also sertraline ) up to 10 mg / kg produced a similar degree of potentiation in both experimental protocols on the 5 - HTP - induced HTR . - pubmed
Furthermore , as in the latter study on the 5 - HTP - induced HTR , in the present investigation nisoxetine potently attenuated whereas GBR 12935 did not modulate the induced HTR . - pubmed
The results show that the respective serotonergic and noradrenergic effects of cocaine also operate on the L - tryptophan - induced HTR . - pubmed
The differential effects of cocaine and sertraline in experimental protocols 1 and 2 on the L - tryptophan- versus 5 - HTP - induced HTRs suggest that cocaine has additional effects on the conversion of L - tryptophan to 5 - HT . - pubmed
The catalytic properties of the proteases were analyzed by their kinetic constants and by a combination of two - dimensional gelatin - sodium dodecyl sulfate - polyacrylamide gel electrophoresis and enzyme overlay membranes . - pubmed
Both Pr2 isoforms preferentially cleave at the carboxyl sides of positively charged amino acids , preferring arginine ; the pI 4.4 Pr2 isoform also possessed significant activity against lysine . - pubmed
The mean ( + /- SD ) values of serum total cholesterol , low - density - lipoprotein ( LDL ) cholesterol , high - density - lipoprotein ( HDL ) cholesterol and triglycerides in the hypercholesterolemic subjects were 7.2 + /- 1.1 mmol / L , 5.1 + /- 1.1 mmol / L , 1.0 + /- 0.1 mmol / L and 2.4 + /- 0.9 mmol / L , respectively . - pubmed
Exposure to MBC resulted in an increased number of spindle poles associated with chromosomes displaced from the metaphase plate . - pubmed
Since platelet - activating factor ( PAF ) is a potent mediator of inflammatory responses , we investigated the potential of PAF to modulate IL-6 and GM - CSF production by EC . - pubmed
Exposure of these cells to PAF resulted in a concentration - dependent increase in IL-6 production , with a maximum at 10(-10 ) M PAF . - pubmed
This effect was specific for PAF since it was prevented by preincubation with the PAF receptor antagonist , WEB 2086 . - pubmed
Pretreatment of EC with the protein synthesis inhibitor cycloheximide before their exposure to PAF resulted , after washout of the cycloheximide , in a markedly augmented production of IL-6 , suggesting a synergy between augmented IL-6 mRNA accumulation by PAF and IL-6 mRNA superinduction by cycloheximide . - pubmed
GM - CSF production by EC was also stimulated by the combined effects of PAF and TNF alpha , but PAF alone did not affect GM - CSF production . - pubmed
Taken together , our data suggest that PAF can stimulate EC to synthesize cytokines , including IL-6 and GM - CSF , which may contribute to local and , possibly , systemic responses during inflammation . - pubmed
0.10 , P & lt ; 0.05 ) , significantly higher levels of oestradiol post - partum ( 0.7 vs. 0.19 ng / ml , + /- azaperone ; S.E.D. - pubmed
gamma - glutamylcysteine synthetase : mRNA stabilization and independent subunit transcription by 4 - hydroxy-2 - nonenal . - pubmed
gamma - Glutamylcysteine synthetase ( GCS ) , the rate - limiting enzyme in de novo glutathione ( GSH ) synthesis , is composed of one catalytic ( heavy ) and one regulatory ( light ) subunit . - pubmed
4HNE increased both the transcription rates and the stability of mRNA for both GCS subunits , resulting in an increased mRNA content for both subunits . - pubmed
Emetine , a protein synthesis inhibitor , blocked the increase in GCS light subunit mRNA but not the increase in GCS heavy subunit mRNA . - pubmed
This suggested that although 4HNE increased transcription and stabilization of both GCS subunit mRNAs , the signaling pathways involved in the induction of the two GCS subunits differed . - pubmed
These findings indicate that IkappaBalpha is imported in the nucleus by a piggy - back mechanism that involves additional protein(s ) containing a basic NLS and able to interact with ankyrin repeats of IkappaBalpha . - pubmed
The purified Ftf exhibited maximum activity at pH 6.0 and 37 degrees C , was activated by Ca2 + , but inhibited by the metal ions Cu2 + , Zn2 + , Hg2 + and Fe3 + . - pubmed
The enzyme catalysed the transfer of the fructosyl moiety of sucrose to a number of acceptors , including water , glucose and sucrose via a Ping Pong mechanism involving a fructosyl - enzyme intermediate . - pubmed
In diabetes , activation of the aldose reductase ( AR ) pathway and alterations of glucose - sensitive signal transduction pathways have been implicated in depletion of intracellular taurine , an endogenous antioxidant and compatible osmolyte . - pubmed
Cellular taurine accumulation occurs by an osmotically induced , protein kinase C ( PKC)-regulated Na (+)- taurine cotransporter ( hTT ) . - pubmed
In low AR - expressing cells , 20 mM glucose decreased taurine content , hTT transporter activity , and mRNA levels , and these effects were unaffected by AR inhibition ( ARI ) . - pubmed
In these cells , the inhibitory effects of high glucose on hTT appeared to be posttranscriptionally mediated , because 20 mM glucose decreased hTT mRNA stability without affecting hTT transcriptional rate . - pubmed
Inhibition of PKC overcame the decrease in hTT activity in high glucose - exposed cells . - pubmed
In high AR - expressing cells , prolonged exposure to 20 mM glucose resulted in intracellular taurine depletion , which paralleled sorbitol accumulation and was prevented by ARI . - pubmed
In 5 mM glucose , hTT transcriptional rate was markedly decreased in high AR - expressing cells , did not decline further in 20 mM glucose , but was increased by ARI to levels above those observed in low AR - expressing cells . - pubmed
Therefore , glucose rapidly and specifically decreases taurine content , hTT activity , and mRNA abundance by AR - unrelated and AR - related posttranscriptional and transcriptional mechanisms . - pubmed
Data including age , gender , cause of end - stage renal disease ( ESRD ) , time on dialysis , body mass index ( BMI ) , blood levels of midweek predialysis albumin , prealbumin , and bicarbonate were collected . - pubmed
Average values ( + /- 1 SD ) were age 61 + /- 16 years , BMI 23.3 + /- 4.6 kg / m2 , dialysis time 12.4 + /- 2.7 h / week , HCO3 22.8 + /- 3.5 mmol / L , albumin 38.7 + /- 5.3 g / L , prealbumin 340 + /- 90 mg / L , Kt / V 1.36 + /- 0.36 , nPCR 1.13 + /- 0.32 g / kg BW / day , and LBM 0.86 + /- 0.21 % of ideal LBM . - pubmed
Bicarbonate was also negatively correlated with albumin , prealbumin , BMI , and LBM . - pubmed
Chalcone isomerase ( CHI ) catalyzes the intramolecular cyclization of chalcone synthesized by chalcone synthase ( CHS ) into ( 2S )- naringenin , an essential compound in the biosynthesis of anthocyanin pigments , inducers of Rhizobium nodulation genes , and antimicrobial phytoalexins . - pubmed
The lower pressure limit of RBF autoregulation was examined before and after infusion of the NOS inhibitor N ( G )- monometyl - L - arginine ( L - NMMA ; 500 microg . - pubmed
As the GFR was unchanged during L - NMMA infusion , these observations suggest that postglomerular contraction during NOS inhibition may be involved in the regulation of GFR in 70 - week - old SHR . - pubmed
Immunoblotting experiments showed that kainate induced more potent expression of c - Fos protein in nuclear fractions obtained 2h after injection than those obtained 18h later , with similar expression between cytosolic fractions obtained 2 and 18h after administration . - pubmed
Two - dimensional electrophoresis revealed expression of several proteins immunoreactive to the anti - c - Fos antibody with different isoelectric points in response to kainate in nuclear and cytosolic fractions of the hippocampus for 2 - 18h after a single injection . - pubmed
Immunoprecipitation analysis using the anti - c - Fos antibody showed phosphorylation of c - Fos protein on serine residues in hippocampal nuclear fractions 2h after administration , with additional phosphorylation on tyrosine residues 18h later . - pubmed
Prior treatment of the protein synthesis inhibitor cycloheximide prevented the expression of immunoreactivities to the anti - c - Fos antibody detected on two - dimensional electrophoresis in hippocampal nuclear fractions obtained 2h after administration . - pubmed
These results suggest that in vivo kainate signals may lead to persistent expression of the transcription factor activator protein-1 that consists of different Fos family members , as well as of c - Fos protein phosphorylated on serine and/or tyrosine residues , at an early stage after administration . - pubmed
The heterotrophic and autotrophic alkaliphiles that grew with thiocyanate as an N source possessed a relatively high level of cyanase activity which converted cyanate ( CNO- ) to ammonia and CO2 . - pubmed
On the other hand , cyanase activity either was absent or was present at very low levels in the autotrophic strains grown on thiocyanate as the sole energy and N source . - pubmed
keratinocyte growth factor was tested by [ 3H ] thymidine assay . - pubmed
The difference in keratinocyte growth factor receptor and androgen receptor gene expression after treatment with and without keratinocyte growth factor and flutamide were determined by quantitative multiplex PCR and quantitated using densitometry analysis . - pubmed
Epithelial cells showed a significant time and dose dependent stimulation of cell proliferation with keratinocyte growth factor and dihydrotestosterone ( p & lt;0.05 ) . - pubmed
keratinocyte growth factor quantitative multiplex PCR revealed stimulated keratinocyte growth factor receptor and androgen receptor messenger RNA expression . - pubmed
CONCLUSIONS : These results show that keratinocyte growth factor up - regulates the keratinocyte growth factor and androgen receptors in the absence of androgen . - pubmed
Thus , the androgen signaling pathway may be activated by growth factors such as keratinocyte growth factor in an androgen deficient environment . - pubmed
Treatment with calcium channel blocker ( CCB)s , dihydropyridines and others , is frequently complicated by dependent oedema in the absence of sodium retention or cardiac failure , a bothersome side effect of unclear aetiology . - pubmed
Differential signalling of both wild - type and Thr(343)Arg dopamine D ( 2short ) receptor by partial agonists in a G - protein - dependent manner . - pubmed
Most of the partial agonists , but not bromocriptine , displayed an enhanced response at the mutant D ( 2short ) receptor , suggesting that the mutation brings the receptor in a more active state . - pubmed
This enhancement was apparent both at the level of G - protein activation ( [ 35S ] GTPgammaS binding ) and at the effector ( Ca ( 2 + ) response ) and occurred with different G ( alpha )- proteins . - pubmed
Lisuride and in particular bromocriptine produced a more potent response in the co - presence of a G ( alphao ) protein instead of a chimeric G ( alphaq / o )- or a promiscuous G ( alpha15 )- protein . - pubmed
S (+)- propylnorapomorphine showed a similar partial response irrespective of the combined G ( alpha )- protein . - pubmed
Bromerguride and ( + ) - UH 232 induced weak ( 16 to 21 % versus dopamine ) intrinsic activity in the co - presence of a G ( alphaq / o )- protein in contrast to their silent properties with a G(alpha15)- or a G ( alphao ) Cys ( 351 ) Ile - protein . - pubmed
Chemical modification of mitochondria with the arginine - specific reagents phenylglyoxal ( PGO ) and 2,3 - butanedione ( BAD ) decreases the Ca ( 2 + ) sensitivity of the permeability transition pore ( PTP ) and stabilizes it in the closed conformation ( Eriksson , O. , Fontaine , E. , and Bernardi , P. ( 1998 ) J. Biol . - pubmed
Unexpectedly , modification of mitochondria with the arginine - specific reagent p - hydroxyphenylglyoxal ( OH - PGO ) resulted instead in PTP opening . - pubmed
Sequential modification with OH - PGO and PGO ( or BAD ) revealed that the effects on the PTP depended on the order of the additions . - pubmed
PTP opening was observed when OH - PGO preceded , and PTP closing was observed when OH - PGO followed , the addition of PGO ( or BAD ) . - pubmed
The differential effects of OH - PGO and PGO on the PTP open probability ( i ) were not modified by the conformation - specific ligands of the adenine nucleotide translocase bongkrekate and atractylate ; and ( ii ) were also observed in de - energized mitochondria , indicating that the effect is exerted directly on the PTP . - pubmed
OH - PGO dramatically sensitized PTP opening , which was triggered by depolarization even in the presence of EGTA . - pubmed
These data show that arginine modification modulates the PTP conformation in a ligand - selective fashion and suggest that the effects of OH - PGO , PGO , and BAD are mediated by the same arginine residues . - pubmed
The results indicate that both OH - PGO and PGO react with arginine at a stoichiometry of 2:1 and form stable adducts that may be feasible to identify the PTP at the molecular level . - pubmed
The minimal requirements for COP I and COP II vesicle formation from liposomes are the small GTPases Arf1 and Sar1 , GTP and the coat complexes themselves . - pubmed
Coat - nucleating factors including specific GTP - GDP exchange factors , SNAREs and other cargo molecules are likely to determine the site of vesicle formation . - pubmed
For two - step addition , previously embryos were 2 min pre - equilibrated in 0.5 ml of ( 1:1 ) PBS plus 20 % FCS : vitrification solution without sucrose . - pubmed
In experiment 2 , we attempted to substitute the use of PBS by HEPES - buffered Ham 's F-10 ( h - CM ) in all cryoprotective solutions or media . - pubmed
When h - CM was used , a significant reduction in the in vitro embryo development was observed when the HEPES - buffered groups were compared with one - step ( 20EG-20DMSO+S ) group in s - PBS ( 35 - 45 % versus 73 % ) . - pubmed
AIMS : To evaluate the suitability of chrome azurol S ( CAS ) agar plate assay as a quantitative methodology for siderophore production . - pubmed
Blood was extracted from the heart to examine the level of LDL - cholesterol . - pubmed
RESULTS : The level of LDL - cholesterol in the high fat diet group was 26.5 + /- 7.49 mmol / L , significantly higher than that in the normal diet group ( 0.71 + /- 0.65 mmol / L , P & lt ; 0.05 ) . - pubmed
OBJECTIVES : To provide context in oncology for the significance of the benefits and cost of combined androgen blockade ( CAB ) in the treatment of advanced prostate cancer . - pubmed
The survival benefit with anastrozole or exemestane in advanced breast cancer , or with the addition of trastuzumab to standard chemotherapy in metastatic breast cancer that overexpresses human epidermal growth factor receptor 2 ( HER2 ) , is approximately 4 to 5 months . - pubmed
The calculated cost per month of survival benefit with bicalutamide in CAB for prostate cancer is 437 US dollars to 1107 US dollars . - pubmed
At steady state , it shows alterations which are not detected by the measurement of the oxygen saturation in the longitudinal sinus or that of CBF . - pubmed
These SRCs interact directly with liganded TR and function as adapter molecules to recruit other coactivators such as p300 / CBP . - pubmed
A combination of chromatographic mobility , immunoassays specific for digoxin and dihydrodigoxin , and deglycosylation using 5 - sulfosalicylic acid were used to identify the DLIF and Dh - DLIF components . - pubmed
RESULTS : With cholesterol as precursor , the cells produced DLIF ( 7.5 mCi / mmol ) with a labeling efficiency of 10 % , whereas with acetate the cells produced DLIF ( 72.2 mCi / mmol ) with a labeling efficiency of 0.08 % of the total DLIF produced . - pubmed
Mevastatin ( 5 micromol ) , an inhibitor of the enzyme hydroxymethylglutaryl - CoA reductase and thus of cholesterol biosynthesis , gave an 85 % decrease in the production of ( 14 ) C - DLIF and progesterone , but only a modest 15 % decrease in ( 14 ) C - Dh - DLIF production . - pubmed
Thioredoxin is associated with proliferation , p53 expression and negative estrogen and progesterone receptor status in breast carcinoma . - pubmed
Positive nuclear immunostaining was associated with negative estrogen and progesterone receptor status . - pubmed
Another is Tim11p , which is required for oligomeric assembly of F1Fo - ATP synthase , which generates ATP through oxidative phosphorylation . - pubmed
Ceramide - induced apoptosis : role of catalase and hepatocyte growth factor . - pubmed
The aim of this study was to elucidate cellular mechanisms involved in ceramide - induced apoptosis and its attenuation by hepatocyte growth factor ( HGF ) . - pubmed
Ceramide - treated cells showed increased caspase-3 activation and an increase in mitochondrial membrane permeability transition ( MPT ) . - pubmed
Furthermore , ceramide treatment significantly decreased catalase enzymatic activity and protein expression . - pubmed
HGF pretreatment ( 20 ng / ml ) significantly inhibited ceramide - induced apoptosis and reduced the accumulation of ROS , the activation of caspase-3 , and the increase in MPT and prevented the reduction in catalase activity and expression . - pubmed
Together , the data suggest that ceramide induces apoptosis in RPE cells by increasing ROS production , MPT , and caspase-3 activation . - pubmed
The ceramide effect is potentiated by H2O2 and associated with a reduction in catalase activity , suggesting that catalase plays a central role in regulating this apoptotic response . - pubmed
Examples are : genistein and EGCG , which effect signaling through the IGF-1 / AKT pathway and resveratrol and limonen , which do so through the RAS pathway . - pubmed
Phenylacetate activates p53 and p21 through inhibition of methyltransferase and farnesylation of the RAS protein . - pubmed
Losartan increased blood levels of BK-(1 - 9 ) and hydroxylated BK-(1 - 9 ) by approximately 2 - fold and reduced the BK-(1 - 7)/BK-(1 - 9 ) ratio by 55 % . - pubmed
Both losartan and eprosartan increased plasma levels of Ang I , Ang II , and Ang -( 2 - 8 ) , and eprosartan increased Ang -( 3 - 8 ) levels . - pubmed
Ang -( 1 - 7 ) and Ang -( 1 - 9 ) levels were unchanged . - pubmed
The reductions in BK-(1 - 7)/BK-(1 - 9 ) , Ang II / Ang I , and Ang -( 1 - 7 )/ Ang I ratios suggest that the increased bradykinin levels were the result of reduced metabolism by ACE and neutral endopeptidase . - pubmed
Increased bradykinin levels may represent a class effect of AT1 receptor blockers that contributes to their therapeutic actions and may also contribute to the angioedema that may accompany this therapy . - pubmed
Mycobacteria produce beta - lactamases and are intrinsically resistant to beta - lactam antibiotics . - pubmed
In addition to the beta - lactamases , cell envelope permeability and variations in certain peptidoglycan biosynthetic enzymes are believed to contribute to beta - lactam resistance in these organisms . - pubmed
Immunofluorescence and ultrathin - section electron microscopy reveals that in Odf2(-/- ) cells , distal / subdistal appendages disappear from mother centrioles , making it difficult to distinguish mother from daughter centrioles . - pubmed
Biochemistry variables were also considered : fasting blood sugar , total cholesterol , HDL - C , LDL - C , triglycerides , fibrinogen , C reactive protein and homocysteine . - pubmed
The overall objective was to compare the efficacy of GnRH , porcine LH ( pLH ) and estradiol cypionate ( ECP ) , in a modified Ovsynch / fixed - time AI ( FTAI ) protocol that included a controlled internal drug [ progesterone ] release ( CIDR ) device . - pubmed
The first and second hormonal treatments ( with an intervening PGF treatment on Day -3 ) were GnRH / GnRH ( 100 microg ) , ECP / ECP ( 0.5 mg ) , pLH / pLH ( 12.5 mg ) or GnRH / ECP , respectively ; pregnancy rates were 58.7 , 66.1 , 45.9 and 48.3 % , respectively ( ECP / ECP>both pLH / pLH and GnRH / ECP ; P<or=0.05 ) . - pubmed
The cellular ATP level at 3 h after the start of SI was increased compared with that at 2 h , and was almost the same as that before the start of SI . - pubmed
The cellular ATP level at 3 h SI was significantly reduced by either the inhibition of nitric oxide synthase ( NOS ) or scavenging of NO . - pubmed
Either the inhibition of NOS or the scavenging of NO during SI for 3 h also resulted in a significant decrease in the survival rate of myocytes . - pubmed
The fluorescent intensity of DAF - FM was significantly increased at 3 h SI as compared with that at 2 h SI , and the increase in DAF fluorescence during SI was almost completely suppressed by treatment with vinyl - L - NIO ( L - VNIO ) , a potent specific inhibitor of nNOS . - pubmed
The inositol 1,4,5 - trisphosphate receptor ( IP ( 3 ) R ) , which forms a homotetrameric Ca2 + release channel in the endoplasmic reticulum , is one of the key molecules responsible for this regulation . - pubmed
To promote the Ca2 + -channel gating and release from the endoplasmic reticulum , IP(3 ) binds to the amino - terminal region of IP ( 3 ) R. - pubmed
OBJECTIVE : To investigate the importance of the phosphorylation of Ser16 and Thr17 sites of phospholamban ( PLN ) on intracellular Ca2 + ( Cai2 + ) handling and contractile recovery of the stunned myocardium . - pubmed
In PLN - WT , a transient increase in Thr17 phosphorylation at early reperfusion preceded a recovery of Ca2 + transient amplitude , virtually completed by the end of reperfusion . - pubmed
In contrast , in PLN - DM , there was a poor recovery of Cai2 + transient amplitude and LVDP was significantly lower ( 28.3+/-6.7 % , n=11 , 30 min reperfusion ) than in PLN - WT hearts . - pubmed
Although myofilament Ca2 + responsiveness and troponin I ( TnI ) degradation did not differ between groups , the episodes of mechanical alternans , typical of Cai2 + overload , were significantly prolonged in PLN - DM vs. PLN - WT hearts . - pubmed
CONCLUSIONS : PLN phosphorylation appears to be crucial for the mechanical and Cai2 + recovery during stunning and protective against the mechanical abnormalities typical of Cai2 + overload . - pubmed
The importance of PLN phosphorylation would primarily reside in the Thr17 residue , which is phosphorylated during the critical early phase of reperfusion . - pubmed
Our results emphasize that , although ablation of PLN phosphorylation does not affect basal contractility , it does alter Ca2 + handling and mechanical performance under stress situations . - pubmed
MEASURES OF OUTCOME : At baseline , and after six and twelve months of supplementation , body composition , bone mineral density , serum 25 OH vitamin D , intact parathyroid hormone ( iPTH ) , osteocalcin , decarboxylated osteocalcin , urinary aminoterminal telopeptide of type I collagen ( NTX ) , deoxypyridoline cross links ( Dpd ) and equol were measured . - pubmed
The group receiving the special supplement had a significant increase in serum 25 OH vitamin D and a significant decrease in serum iPTH and decarboxylated osteocalcin . - pubmed
Polyurethane foam unit ( PFU ) systems were collected from 11 lakes and three rivers in the Yunnan Plateau , China and , the PFU extrusion liquids were analyzed for organochlorine pesticides ( OCPs ) by gas chromatography with electron capture detection ( GC - ECD ) . - pubmed
At the end of reperfusion , histopathological changes of intestine were observed , and malondialdehyde ( MDA ) levels , superoxide dismutase ( SOD ) , catalase ( CAT ) , and glutathione peroxidase ( GSH - Px ) activities in intestinal tissues were also detected . - pubmed
RESULTS : The H group showed significantly lower MDA levels and higher activities of SOD , CAT , and GSH - Px in the intestinal tissue compared with the IR group . - pubmed
G quadruplex - based FRET probes with the thrombin - binding aptamer ( TBA ) sequence designed for the efficient fluorometric detection of the potassium ion . - pubmed
The dual - labeled oligonucleotide derivative , FAT-0 , carrying 6 - carboxyfluorescein ( FAM ) and 6 - carboxytetramethylrhodamine ( TAMRA ) labels at the 5 ' and 3 ' termini of the thrombin - binding aptamer ( TBA ) sequence 5'-GGT TGG TGT GGT TGG-3 ' , and its derivatives , FAT - n ( n=3 , 5 , and 7 ) with a spacer at the 5'-end of a TBA sequence of T(m)A ( m=2 , 4 , and 6 ) have been designed and synthesized . - pubmed
Although larger studies are needed , this case demonstrates that pulsed - dosing with OSPB enhances HbF synthesis . - pubmed
To deliver and retain viable repair cells in a surgically prepared cartilage lesion , we previously developed an adhesive in situ - gelling cell carrier by suspending cells in a solution of hydroxyethyl cellulose ( HEC ) , which was then mixed with chitosan - glycerol phosphate to form a chitosan - GP / HEC gel . - pubmed
Monolayer cells survived and proliferated in up to 1 mM of glyoxal , however only a very narrow range of glyoxal concentration in chitosan - GP / HEC , 0.1 - 0.15 mM , permitted gel formation , cell survival , and cell proliferation . - pubmed
Chitosan gels containing HEC required slightly less glyoxal to solidify . - pubmed
Rin is activated following stimulation of both Gsalpha and Gialpha cascades but does not rely upon cyclic AMP ( cAMP)- , Ca ( 2 +)- , or Epac - dependent signaling pathways . - pubmed
RNA interference - mediated HSP27 silencing suppresses both PACAP38- and Rin - mediated neurite outgrowth , while expression of a constitutively active Rin mutant increases both HSP27 protein and phospho - HSP27 levels , supporting a role for Rin - HSP27 signaling in neuronal differentiation . - pubmed
A novel method of determining portal systemic shunting using biodegradable 99Tcm labelled albumin microspheres . - pubmed
The clinical effects of two groups were evaluated after one - week treatment and uric acid ( UA ) and C - reactive protein ( CRP ) levels in blood were determined after 1 - month treatment . - pubmed
This review summarizes evidence that dendritic cells are a part of the mononuclear phagocyte system and are derived from a common precursor , responsive to the same growth factors ( including CSF-1 ) , express the same surface markers ( including CD11c ) , and have no unique adaptation for Ag presentation that is not shared by other macrophages . - pubmed
Moreover , the expression of Bcl-2 mRNA and the protein expressions of MDR1 , LRP , and P - gp were decreased when compared with those of DDP groups , respectively . - pubmed
The effects may be associated with over - expression of MDR1 , LRP , P - gp , and Bcl-2 , which can increase the intracellular platinum accumulation and induce the cell apoptosis . - pubmed
We recently showed that Slc26a9 has both nCl (-)- HCO ( 3 )(- ) exchanger and Cl (- ) channel function . - pubmed
Deletion of the Slc26a9 STAS domain ( a9 - DeltaSTAS ) virtually eliminated the Cl (- ) currents with only a modest affect on nCl(-)-HCO ( 3 )(- ) exchange activity . - pubmed
These results imply that Slc26 - STAS domains may all interact with ( R)CFTR but that the physiological outcome is specific to differing Slc26 proteins , allowing for dynamic and acute fine tuning of ion transport for various epithelia . - pubmed
Although methyl bromide ( MB ) has been phased out in many agricultural industries , it is still the preferred treatment in the perennial nursery industry and is used under Critical Use Exemptions and Quarantine / Preshipment provisions of the Montreal Protocol . - pubmed
RESULTS : Reduced rates ( 100 - 260 kg ha(-1 ) ) of MB applied in combination with chloropicrin ( Pic ) and sealed with a low - permeability plastic film provided weed and nematode control similar to the industry standard rate of 392 kg ha(-1 ) MB : Pic ( 98:2 ) sealed with high - density polyethylene ( HDPE ) film . - pubmed
While the water extract of viscous material showed a higher ACE inhibitory activity than the respective ethanol extract . - pubmed
INTERVENTION(S ): Cetrorelix is used as a GnRH - antagonist in ART treatments . - pubmed
To assess the effect of delayed ovulation on embryonic development , cetrorelix was applied concomitantly with follicle stimulation by pregnant mare serum gonadotropin . - pubmed
The origin of NO signaling can be traceable back to the origin of life with the large scale of parallel evolution of NO synthases ( NOSs ) . - pubmed
While in early preconditioning NO is produced by the endothelial and neuronal isoform of NO synthase , in delayed preconditioning NO is synthesized by the inducible or ' immunological ' isoform of NO synthase . - pubmed
Furthermore , in early preconditioning , NO acts through the canonical cGMP pathway , possibly through protein kinase G and opening of mitochondrial K(ATP ) channels . - pubmed
In late preconditioning , the protection is mediated by peroxynitrite formed by the reaction of NO with superoxide derived from the enzyme NADPH oxidase . - pubmed
The mechanisms by which peroxynitrite exerts its protective effect may include improvement of post - ischaemic cerebrovascular function , leading to enhancement of blood flow to the ischaemic territory , and expression of prosurvival genes resulting in cytoprotection . - pubmed
The levels of parathyroid hormone , calcitonin , osteocalcin , procollagen carboxy - terminal propeptide and bone alkaline phosphatase were significantly lower in the Al - treated group than in the control group from days 90 , 30 , 60 , 60 and 90 , respectively . - pubmed
Argiotoxin in the closed AMPA receptor channel : experimental and modeling study . - pubmed
Binding of argiotoxin in the closed state of Ca ( 2 +)-permeable AMPA receptor channels was studied using electrophysiological and molecular modeling approaches . - pubmed
Experimental study unambiguously revealed that argiotoxin is trapped in the closed AMPA receptor channels after agonist dissociation . - pubmed
The unique sea urchin - type morphology , bright and sharply visible emission bands of lanthanide , and large aspect ratio of ZnO crystalline nanotips make this novel composite an excellent signal receiver , waveguide , and emitter . - pubmed
In addition , patients on the etravirine arm had fewer AIDS - defining conditions , hospitalizations , and lower mortality compared with the OBR control arm . - pubmed
Hydrogen sulfide inhibits the translational expression of hypoxia - inducible factor-1alpha . - pubmed
BACKGROUND AND PURPOSE : The accumulation of hypoxia - inducible factor-1alpha ( HIF-1alpha ) is under the influence of hydrogen sulfide ( H ( 2 ) S ) , which regulates hypoxia responses . - pubmed
The regulation of HIF-1alpha accumulation by H ( 2 ) S has been shown , but the mechanisms for this effect are largely elusive and controversial . - pubmed
This study aimed at addressing the controversial mechanisms for and the functional importance of the interaction of H ( 2 ) S and HIF-1alpha protein . - pubmed
The mechanisms for H ( 2 ) S - regulated HIF-1alpha protein levels were determined using short interfering RNA transfection , co - immunoprecipitation and 7 - methyl - GTP sepharose 4B pull - down assay . - pubmed
KEY RESULTS : The accumulation of HIF-1alpha protein under hypoxia ( 1 % O ( 2 ) ) or hypoxia - mimetic conditions was reversed by sodium hydrosulfide ( NaHS ) . - pubmed
This effect of NaHS was not altered after blocking the ubiquitin - proteasomal pathway for HIF-1alpha degradation ; however , blockade of protein translation with cycloheximide abolished the effect of NaHS on the half - life of HIF-1alpha protein . - pubmed
Knockdown of eukaryotic translation initiation factor 2alpha ( eIF2alpha ) suppressed the effect of NaHS on HIF-1alpha protein accumulation under hypoxia . - pubmed
NaHS inhibited the expression of VEGF under hypoxia . - pubmed
CONCLUSIONS AND IMPLICATIONS : H ( 2 ) S suppresses HIF-1alpha translation by enhancing eIF2alpha phosphorylation under hypoxia . - pubmed
The interaction of H ( 2 ) S and HIF-1alpha inhibits the angiogenic activity of vascular endothelial cells under hypoxia through the down - regulation of VEGF . - pubmed
In addition to the provision of correct information and the correction of misinformation , clinicians must be aware of and must address barriers to adherence with AD therapy , especially parent anxiety about the safety of topical medications ( corticosteroids and topical calcineurin inhibitors ) . - pubmed
Genetic studies , however , have allowed to clarify which genetic variations underlie the adverse response to some antiretroviral drugs ( such as HLA - B*5701 in the treatment with abacavir ) or the occurrence of renal complications as the disease progresses . - pubmed
Altered nucleotide - microtubule coupling and increased mechanical output by a kinesin mutant . - pubmed
Kinesin motors hydrolyze ATP to produce force and do work in the cell -- how the motors do this is not fully understood , but is thought to depend on the coupling of ATP hydrolysis to microtubule binding by the motor . - pubmed
Mutants show weak - ADP / tight - microtubule binding , instead of tight - ADP / weak - microtubule binding like wild type -- they hydrolyze ATP faster than wild type , move faster in motility assays , and assemble long spindles with greatly elongated poles , which are also produced by simulations of assembly with tighter microtubule binding and faster sliding . - pubmed
The plasma sample was pretreated by protein precipitation with acidified acetonitrile . - pubmed
Since these tumors are dependent upon the PI3 K pathway , we investigated the potential for tumor response by the targeting of this pathway with rapamycin , an mTOR inhibitor . - pubmed
FDG dual hybrid PET / CT imaging demonstrated a dramatic tumor response in the rapamycin arm and this was confirmed on necropsy . - pubmed
The tumor tissue remaining after treatment with rapamycin demonstrated increased pERK1 / 2 or persistent phosphorylated ribosomal protein S6 ( pS6 ) , indicating potential resistance mechanisms . - pubmed
EGFR tyrosine kinase inhibitors ( TKIs ) such as gefitinib induce apoptosis of cancer cells , but prolonged treatment is often associated with acquired resistance . - pubmed
Here , we identified a novel MEK1 / 2 inhibitor , CZ0775 , and compared its cytotoxic effects to those of AZD6244 ( selumetinib ) in non - small cell lung cancer ( NSCLC ) cell lines harboring EGFR mutations . - pubmed
Our results suggest that combination treatment with CZ0774 and EGFR inhibitors is a promising therapeutic approach for the treatment of EGFR - TKI - resistant lung cancers and its effect is mediated by the inhibition of ERK and the induction of BIM . - pubmed
Comparison of the effects of albumin 5 % , hydroxyethyl starch 130/0.4 6 % , and Ringer ' s lactate on blood loss and coagulation after cardiac surgery . - pubmed
INTRODUCTION : Cariprazine is an orally active and potent D3 and D2 partial agonist with preferential binding to D3 receptors in development for the treatment of schizophrenia and bipolar mania . - pubmed
Next , using oocytes subjected to IVM for 12 - 30 hr , sperm penetration was examined after 4 - 18 hr of in vitro fertilization ( IVF ) . - pubmed
A significant negative correlation between IVM duration and the timing when 50 % of oocytes were penetrated by sperm after IVF start was observed ( P<0.01 ) . - pubmed
In conclusion , under the present IVM / IVF conditions , it was estimated that oocytes acquired their highest developmental competence at about 30 hr after IVM start , and thus , the optimal IVM duration was calculated to be about 21 hr . - pubmed
Treatment with 50 - 100 nmol / L ZD 7288 significantly inhibited EMG responses ( 16%-62 % , 80 - 20 mmHg CRD , P & lt ; 0.05 ) and AWR scores ( 24%-37 % , 40 - 20 mmHg CRD , P & lt ; 0.05 ; 12%-61 % , 80 - 20 mmHg CRD , P & lt ; 0.05 , respectively ) , and significantly increased pain thresholds ( 32%-77 % , P & lt ; 0.05 ) . - pubmed
Renin - angiotensin - aldosterone system ( RAAS ) activation occurs in hyperthyroidism , which could cause peri - tubular hypoxia , tubular damage and the development of aCKD . - pubmed
Urinary vascular endothelial growth factor : creatinine ratio ( VEGFCR ) is postulated to be a marker of tubular hypoxia . - pubmed
Direct evidence of spatially selective iron mineralization using an immobilized ferritin protein cage . - pubmed
Here , we conducted quantitative and kinetic analyses of the mineralization behavior of immobilized ( apo)ferritin on a polyelectrolyte multilayer ( PEM ) using quartz crystal microbalance ( QCM ) , scanning electron microscopy ( SEM ) , and X - ray photoelectron spectroscopy ( XPS ) techniques . - pubmed
We demonstrated that the ( apo)ferritin immobilized on a substrate synthesizes a ferrihydrite core within the confines of the protein cage ; similar to a solution dispersed system . - pubmed
In addition , we applied a ferritin / apoferritin blended monolayer to the study of iron mineralization and revealed that biomineralization in this system is spatially selective . - pubmed
Pyridine analogues of spirocyclic sigma1 receptor ligands . - pubmed
Spirocyclic benzopyrans 2 interact with high affinity and selectivity with sigma1 receptors . - pubmed
Bioisosteric replacement of the benzene ring of the benzopyran substructure with the electron rich thiophene ring ( 3 ) led to increased sigma1 affinity . - pubmed
The most promising sigma1 ligand within this class of compounds is the pyranopyridine 15 with a double bond in the pyran ring revealing a Ki - value of 4.6 nM and a very high selectivity ( & gt;217 - fold ) over the sigma2 subtype . - pubmed
CLA and CLNA have isomer - specific , health - promoting properties , including anticarcinogenic , antiatherogenic , anti - inflammatory , and antidiabetic activity , as well as the ability to reduce body fat . - pubmed
macrocarpon ( fruits as well as leaves ) resulted in a statistically significant decrease in total cholesterol , LDL - cholesterol , VLDL - cholesterol , and triglycerides in the treated groups compared with the untreated hypercholesterolemic group , regardless of the administrated doses . - pubmed
A significant increase in HDL - cholesterol was observed in the treated groups . - pubmed
RESULTS : In JN PVP group , mean of 3.26 cc of polymethylmethacrylate ( PMMA ) were injected and 4.07 cc in BVF PVP group ( p<0.001 ) . - pubmed
No significant difference in improvement of VAS score was observed between JN and BVF PVP groups ( p=0.43 ) . - pubmed
Usage of BVF for PVP may be an alternative to JN PVP in selected cases . - pubmed
alcohol challenge ( final target breath alcohol concentration = 0.06 g / dl ) and reported on SR and craving . - pubmed
At baseline , LMD and AD reported greater stimulation than HD , which carried over post - alcohol administration . - pubmed
Urinary albumin to creatinine ( ACR ) and beta2 microglobulin to creatinine ratios ( BCR ) are the surrogate and robust markers of renal glomerulopathy and tubulopathy , respectively . - pubmed
ACR & gt ; 300 mg / g creatinine and BCR & gt ; 300 mug / g creatinine were used to define the renal glomerular and tubular damages , respectively . - pubmed
Ethanol - induced GABAA receptor alpha4 subunit plasticity involves phosphorylation and neuroactive steroids . - pubmed
GABAA receptors containing alpha4 subunits are widely implicated in acute ethanol sensitivity , and their spatial and temporal regulation prominently contributes to ethanol - induced neuroplasticity in hippocampus and cortex . - pubmed
However , it is unknown if alpha4 - containing GABAA receptors in the thalamus , an area of high alpha4 expression , display similar regulatory patterns following ethanol administration , and if so , by which molecular mechanisms . - pubmed
In the current study , thalamic GABAA receptor alpha4 subunit levels were increased following a 6 - week- , but not a 2 - week chronic ethanol diet . - pubmed
Following acute high - dose ethanol administration , thalamic GABAA receptor alpha4 subunit levels were regulated in a temporal fashion , as a decrease was observed at 2h followed by a delayed transient increase . - pubmed
PKCgamma and PKCdelta levels paralleled alpha4 temporal expression patterns following ethanol exposure . - pubmed
Initial decreases in alpha4 subunit expression were associated with reduced serine phosphorylation . - pubmed
Delayed increases in expression were not associated with a change in phosphorylation state , but were prevented by inhibiting neuroactive steroid production with the 5alpha - reductase inhibitor finasteride . - pubmed
Overall , these studies indicate that thalamic GABAA receptor alpha4 subunit expression following acute and chronic ethanol administration exhibits similar regulatory patterns as other regions and that transient expression patterns following acute exposure in vivo are likely dependent on both subunit phosphorylation state and neuroactive steroids . - pubmed
Complete refolding of beta1 - adrenergic receptor occurs in n - decyl - beta - D - maltoside ( DM ) micelles from a urea - denatured state , as shown by regain of its original helical structure , ligand binding and protein fluorescence . - pubmed
G protein - coupled receptor kinases ( GRKs ) are a family of serine / threonine protein kinases that terminate G protein - coupled receptor ( GPCR ) signaling by phosphorylating the receptor and inducing its internalization . - pubmed
Clinical and genetic features of Argentinian children with diabetes - onset before 12months of age : Successful transfer from insulin to oral sulfonylurea . - pubmed
Among children with diabetes diagnosed between 6 and 12months , 1 patient had an INS mutation and one patient was homozygous for an SLC19A2 mutation which confirmed a diagnosis of thiamine - responsive megaloblastic anaemia syndrome . - pubmed
Five of the patients with an ABCC8 or KCNJ11 mutation have successfully transferred from insulin to glibenclamide whist 1 child demonstrated a partial response to sulfonylurea treatment . - pubmed
Moreover , L - pGlu -( 1 - benzyl )- l - His - l - Pro - NH2 resulted in a substantial reduction in CA1 ( Cornus Ammonis 1 ) hippocampal neuronal cell death , inflammatory cytokines , TNF - alpha , IL-6 and oxidative stress in hippocampus . - pubmed
Mechanistically , phosphorylation of MEK1 / 2 and ERK1 / 2 in NPCs was enhanced upon LXR agonist treatment , while abrogation of MEK / ERK phosphorylation by the inhibitors PD98059 and U0126 impaired the proliferation of wildtype NPCs in the presence or absence of LXR agonists . - pubmed
Collectively , our findings suggest that LXR agonists GW3965 and LXR623 can stimulate the NPC proliferation in LXR- and MEK / ERK - dependent manner . - pubmed
Endothelial function was also improved , as measured by increased nitric oxide ( NO ) formation and decreased endothelin ( ET-1 ) protein expression . - pubmed
Although the incorporation of AZH was minimally invasive for PDZ3 , structural analysis of NNAA - modified proteins through the methodology presented herein should be performed to ensure structural integrity of the studied target . - pubmed
A positive lymphocyte transformation test ( LTT ) confirmed the implication of amlodipine in the reaction and showed positivity to another calcium channel blocker , nifedipine , demonstrating cross reactivity . - pubmed
CONCLUSION : This is the first reported case of hypersensitivity to amlodipine in which the involvement of the drug is confirmed by a positive TTL . - pubmed
It has been shown that the optimum condition for the modification of MNPs is the reaction at 40 C with an equimolar amount of PMIDA for 3.5 h. The modified MNPs were characterized by X - ray diffraction , transmission electron microscopy , Fourier transform infrared spectroscopy , thermogravimetric , and CHN elemental analyses . - pubmed
The possibility of using colloidal solutions of PMIDA - modified MNPs as a T2 contrast agent for liver studies in vivo ( at a dose of 0.6 mg kg-1 ) was demonstrated for the first time . - pubmed
To experimentally prove the theoretical prediction , iodine - doped graphene materials were prepared by one - pot hydrothermal and ball - milling approaches to realize different doping - sites . - pubmed
Eight PFASs were measured in plasma using isotope dilution ultra - performance liquid chromatography / tandem mass spectrometry ( UPLC - MS / MS ) . - pubmed
Bilirubin and hepatic enzymes alanine aminotransferase ( ALT ) , alkaline phosphatase ( ALP ) , and gamma - glutamyltransferase ( GGT ) were determined in serum using an immunoassay methodology . - pubmed
RESULTS : The changes in majority of PFAS concentrations were positively associated with the changes in activity of ALT , ALP , and GGT and inversely associated with the changes in circulating bilirubin after adjustment for gender and the time - updated covariates LDL- and HDL - cholesterol , serum triglycerides , BMI , statin use , smoking , fasting glucose levels and correction for multiple testing . - pubmed
RESULTS : The LP and MIX silages had lowest pH ( p<0.001 ) on 7 and 48 days , while MIX and CON silages had greatest lactate concentrations ( p<0.05 ) on 7 and 48 days , respectively . - pubmed
Moreover , introducing LDHA mRNA into cells over - expressing miR-33b attenuated the inhibitory effect of miR-33b on the growth and glucose metabolism in NSCLC cells . - pubmed
Taken together , these results confirm that miR-33b is an anti - oncogenic miRNA , which inhibits NSCLC cell growth by targeting LDHA through reprogramming glucose metabolism . - pubmed
The first one - pot synthesis of undoped and Eu doped beta - NaYF4 nanocrystals and their evaluation as efficient dyes for nanomedicine . - pubmed
The expression of the tumor necrosis factor - ( TNF - ) alpha , interleukin - ( IL - ) 1beta , IL-6 , cyclic adenosine monophosphate ( cAMP ) , cyclic guanosine monophosphate ( cGMP ) , phosphodiesterase 2A ( PDE2A ) , and nuclear factor - ( NF - ) kappaB / p65 in spinal dorsal horns was measured by enzyme - linked immunosorbent assay , polymerase chain reaction , and western blot on day 7 after surgery . - pubmed
The determined optimum conditions for cell growth of strain FA - HZ1 were a temperature of 30 C , pH 5.0 , rotation rate of 200 rpm and 0.1 mM DBF as a carbon source . - pubmed
The biochemical and physiological features as well as usage of different carbon sources by FA - HZ1 were studied . - pubmed
The new strain was positive for arginine double hydrolase , gelatinase and citric acid , while it was negative for urease and lysine decarboxylase . - pubmed
FA - HZ1 metabolizes DBF by both the angular and lateral dioxygenation pathways . - pubmed
In addition , the strain FA - HZ1 can also degrade halogenated analogues such as 2 , 8 - dibromo dibenzofuran and 4 -( 4 - bromophenyl ) dibenzofuran . - pubmed
METHODS : The gonadotropin - releasing hormone ( GnRH ) stimulation test was used to determine the HPG axis status and categorize subjects into two groups ( HPG+ or HPG- group ) . - pubmed
In this study , two novel composite membranes containing nanoscale ZIF-8 and polyphenylene sulfide ( PPS ) nonwoven fabric were prepared via hydrothermal ( PPS - ZIF-8 ) and biomimetic mineralization ( PPS - ZIF-8 - BSA ; BSA , bovine serum albumin ) approaches . - pubmed
As for poorly soluble low toxic ( PSLT ) nanomaterials , the US National Institute for Occupational Safety and Health estimated the occupational exposure limit ( OEL ) for titanium dioxide ( TiO2 ) based on a particle surface area ( SA ) metric . - pubmed
Severe arterial hypertension and hyperandrogenism in a boy : a rare case of catecholamine- and beta - HCG - secreting pheochromocytoma . - pubmed
Increased beta - human chorionic gonadotropin ( beta - HCG ) and pubertal testosterone values with suppressed values of luteinizing hormone ( LH ) and follicle - stimulating hormone ( FSH ) suggested an androgenization secondary to HCG hyperproduction . - pubmed
Postoperative resolution of symptoms of beta - HCG and catecholamine excess and normalization of hormonal levels confirmed the diagnosis . - pubmed
Blood samples were taken to assess glucose , insulin , estrogen , and progesterone levels . - pubmed
Despite significant increases in blood glucose and insulin ( P & lt ; 0.001 ) , both central and peripheral arm PWV remained unchanged across time and phase , indicating that neither acute hyperglycemia nor menstrual phase had an impact on central or peripheral arm arterial stiffness . - pubmed
The most frequently prescribed regimens were fludarabine + cyclophosphamide + rituximab ( FCR , 29.3 % ) , bendamustine + rituximab ( BR , 17.7 % ) , and other rituximab - containing regimens ( 22.0 % ) in the treatment - naive setting ( n = 604 ) , other non - FCR / BR rituximab - containing regimens ( 38.7 % ) and non - rituximab - containing regimens ( 28.5 % ) in the relapsed / refractory setting ( n = 945 ) . - pubmed
METHODS : In this cross - sectional study , 310 patients with newly diagnosed cancer who were referred to a university hospital in Turkey over a 6 - month period and 218 age - matched healthy individuals as controls were evaluated in terms of anemia : complete blood count ( CBC ) , ferritin , transferrin saturation ( TS% ) , serum iron ( SI ) , cobalamin ( B12 ) , and folate levels . - pubmed
Folate and B12 deficiencies were seen more frequently in the cancer group than in the controls [ 6.5 % and 0.9 % ( p < 0.001 ) ; 39.3 % and 18.9 % ( p < 0.05 ) , respectively ] . - pubmed
Thus , re - expression of NAA80 , which leads to re - establishment of actin 's N - terminal acetyl group , rescued the Golgi fragmentation , whereas a catalytic dead NAA80 mutant could neither restore actin Nt - acetylation nor Golgi structure . - pubmed
After quercetin treatment , cellular senescence and apoptosis were evaluated by TUNEL staining , X - Gal staining and indirect peroxidase technique for immunocytochemical analysis of related proteins such as Bax , Bcl-2 , caspase-3 , Hsp27 , Lamin B1 , p16 , cyclin B1 . - pubmed
Cell viability was assessed by WTS-1 assay while apoptotic studies were carried out by DAPI , acridine orange ( AO)/ethidium bromide ( EB ) , annexin V + propidium iodide ( PI ) staining using fluorescence microscopy and flow cytometry . - pubmed
7 - Methoxyheptaphylline also caused S - phase cell cycle arrest with concomitant concentration - dependent decline in the expression levels of cyclin A , E and D1 . - pubmed
It was also seen that increasing doses of 7 - Methoxyheptaphylline led to a dose - dependent decline in the expression levels of wnt-13a and beta - catenin . - pubmed
The implementation of trans - fucosylation patterns investigated in this paper via well - designed fucosidase mutants and proper reaction conditions may lead to development of an excellent platform , serving both fundamental studies and industrial - scale processes , for valuable carbohydrates synthesis . - pubmed
Expression of growth factors ( insulin - like growth factor ( IGF ) 1 , IGF2 , epidermal growth factor and transforming growth factor - alpha ) and their receptors was evaluated , with similar expression patterns seen for embryos in sealed culture ( 5 % O2 , embryo density of 1:2 ) compared with the control group ( embryos cultured in microdrops and placed in a 37 C , 5 % CO2 water - jacketed incubator ; P>0.05 ) . - pubmed
In general , intranasal steroid ( INS ) and oral steroid for short time help improve the recovery of the olfactory function in case of olfactory dysfunction after virus infection . - pubmed
After the third dose of durvalumab , his serum CK level was elevated , and soon thereafter myalgia of the proximal muscles and blepharoptosis were observed . - pubmed
In aortic rings , CmV caused a direct vasoconstrictor effect mediated by alpha-1 adrenergic receptors and counteracted by nitric oxide . - pubmed
While a role for hypoxia and hypoxia inducible factor 1alpha has been demonstrated , the contribution of lipid microenvironment and more specifically oxysterols is yet to be explored . - pubmed
Chromatin immunoprecipitation analysis confirms the implication of LXR in the regulation of GLUT1 and HK2 CONCLUSIONS AND IMPLICATIONS : While our work supports a role of oxysterols and LXR in the modulation of macrophage metabolism in atheroma plaques , it also highlights some LXR - independent effects of plaques samples . - pubmed
Stable microtubules ( as defined by resistance to Ca2 + , drug or cold temperature induced disassembly ) form in abundance during tubulin assembly in brain crude extracts . - pubmed
All activity binds to cation exchangers and to Ca ( 2 +)-calmodulin affinity columns . - pubmed
Microtubule stabilization by MBP and STOP220 is abolished in the presence of Ca(2+)-calmodulin , and inhibition curves are similar for both proteins. - pubmed
These data suggest that calcium ions , both those present in the extracellular medium and those bound to the plasma membrane , are required for isoproterenol - stimulation of adenylate cyclase . - pubmed
In addition , it appears that neither the presence of extracellular calcium ions nor full activation of adenylate cyclase are required for desensitization . - pubmed
The development of ACTH - induced hypertension was prevented by both nisoldipine , a calcium channel blocker , and minoxidil , a vascular smooth muscle relaxant . - pubmed
Nisoldipine administration was also found to reverse established ACTH hypertension . - pubmed
Expression of the nifA gene is induced in the symbiotic state and site - directed mutagenesis experiments indicate that nifA expression is essential for symbiotic nitrogen fixation . - pubmed
Non - deleterious inhibition of endogenous peroxidase activity ( EPA ) by cyclopropanone hydrate : a definitive approach . - pubmed
Endogenous peroxidase activity ( EPA ) poses a serious problem in immunoperoxidase localization of antigens unable to withstand deleterious effects of aldehyde fixatives , alcohols , and various oxidative reagents . - pubmed
However , the precise mechanism of the action of these compounds on EPA generating proteins is not understood . - pubmed
Cyclopropanone hydrate is a compound whose inhibitory action on the heme moiety of horseradish peroxidase is well understood . - pubmed
In addition , any gross deleteriousness of cyclopropanone hydrate towards immunoperoxidase immunolocalization of three of the most delicate lymphocyte surface antigens was investigated . - pubmed
Plasma ACTH followed the same trend although it reached significance after ether stress only . - pubmed
Levels of corticosteroid binding globulin were also increased , whereas daily circadian rhythm of serum CORT was blunted . - pubmed
No significant modifications in plasma insulin , plasma glucose and transglutaminase activity in red cells was induced by glibenclamide perfusion . - pubmed
Nevertheless , glibenclamide induced a significant decrease ( p less than 0.005 ) in transglutaminase activity after 20 min of perfusion ( 629.83 + /- 53.08 and 521.18 + /- 43.92 , mean + /- SE , at 0 and 20 min ) . - pubmed
No correlation was observed between glucose or insulin plasma levels and transglutaminase activity . - pubmed
Participation of previously described carcinogen - binding proteins such as the Ah receptor , h2 - 5S protein , 4 - 5S receptor for 3 - methylcholanthrene and the Z - protein fraction was discounted on the grounds of competition binding studies and gel - permeation chromatography . - pubmed
Aflatoxin B1 co - eluted with the glutathione S - transferases during gel - permeation and separation of the various isozymes by cation - exchange chromatography indicated interactions with the YaYa and YaYc - forms . - pubmed
The experiments described here were carried out in order to investigate whether or not the lectin plays any role in mediating the haemocytic response , by measuring the in vivo cellular response to galactose- or sialic acid - rich glycoproteins , either in solution or conjugated to Sepharose beads . - pubmed
Comparison of the ability of injected solutions to stimulate haemocytic aggregates ( nodules ) showed that PSM , whether native , desialylated or pronase - digested to produce small galactose - containing fragments , stimulated large numbers of nodules , in contrast to either sialic acid - rich glycoproteins such as bovine submaxillary mucin ( BSM ) and fetuin , or the protein bovine serum albumen ( BSA ) . - pubmed
Multicellular capsules formed around Sepharose conjugated to galactose - rich molecules such as PSM , asialo - PSM , asialo - fetuin or asialo - BSM were highly significantly thicker than those formed around control untreated beads , whereas capsules around BSA , fetuin or BSM conjugates were significantly thinner . - pubmed
We postulate that a very long chain fatty acyl CoA ( VLCFA CoA ) synthetase , which is different from long chain fatty acyl CoA synthetase , is required for the effective conversion of C24:0 acid to C24:0 CoA. - pubmed
The kinetic mechanism of the binding and release of calcium by troponin and by the complexes troponin : tropomyosin , troponin : tropomyosin : actin , and troponin ( TN)-tropomyosin ( TM)-actin : myosin subfraction 1 ( SF-1 ) was investigated using troponin labeled on the TN - I subunit with the fluorophore 4 -( N - iodoac etoxyethyl - N - methyl )- 7 - nitrobenz-2 - oxa-1,3 - diazole . - pubmed
Kinetic measurements on TN or TN : TM fitted a single rate process for association or dissociation which is consistent with a model in which the calcium sites are equivalent and independent and each calcium induces a change in structure of the complex . - pubmed
TN : TM : actin gave biphasic transients for association and dissociation of calcium . - pubmed
The evidence is interpreted in terms of a model in which regulated actin can exist in two states and the binding of each calcium and SF-1 displaces the equilibrium between states . - pubmed
Saturation of actin sites with SF-1 had no effect on the rate constant for association with TN : TM in the presence of calcium . - pubmed
A specific and sensitive determination of gamma aminobutyric acid in CSF and brain tissue by gas chromatography - mass spectrometry . - pubmed
The accurate measurement of the kinetics of binding of 5 alpha - dihydrotestosterone to the Sertoli cell specific protein , androgen binding protein ( ABP ) , has been frustrated by the extremely rapid rate of dissociation of the ABP - dihydrotestosterone complex . - pubmed
We describe a rapid and highly sensitive assay suitable for ABP quantitation which utilizes DEAE Bio - Gel and [ 3H ] dihydrotestosterone . - pubmed
The assay has been used to accurately measure the rate of dissociation ( 8.25 X 10(-4 ) s-1 , t1/2 14 min ) and the rate of association ( 2.04 X 10(5 ) M s-1 ) of the binding of [ 3H ] dihydrotestosterone to rat ABP . - pubmed
This multipoint assay is extremely rapid such that binding can be measured at equilibrium , it has high precision ( coefficient of variation 3 % ) , and is particularly useful at low protein concentrations ( 50 ng / ml ) ; furthermore , the assay background of nonspecific 3H - binding is extremely low ( 0.2 % ) . - pubmed
Since at such low protein concentrations a 10 point Scatchard analysis can be performed on 1 ml culture medium containing as little as 3 fmol ABP , the assay is suitable for monitoring changes in ABP secretion resulting from manipulations of cells in culture . - pubmed
Progesterone ( P ) and oestradiol-17 beta ( E2 ) were determined from the blood samples by RIA . - pubmed
No changes in blood pressure were observed after vasopressin administration , although the ovarian blood flow quickly decreased in parallel with the secretion of P and E2 . - pubmed
A similar decapeptide , neurokinin B , was also synthesized and conjugated onto BSA using an alternative SH - introducing reagent , 3 -( S - p - methoxybenzyl - thiopropionyl )- thiazolidine-2 - thione , and the above heterobifunctional conjugating reagent . - pubmed
Effect of 17 - estradiol on pituitary gonadotropins and luteal function in gilts . - pubmed
Comparison of the distribution of the proteins in sucrose versus metrizamide gradients suggested that P105 was removed from the free ribonucleoprotein particles before complexing with the ribosomes . - pubmed
These apolipoproteins were purified by DEAE - cellulose chromatography in buffers containing Triton X-100 . - pubmed
In addition , the apolipoproteins showed one band with an apparent molecular weight of 72 000 - 73 000 on SDS - polyacrylamide gel electrophoresis . - pubmed
Even though the apolipoproteins of both species had very similar amino acid compositions , including a relatively high glycine content , no immunologic cross - reactivity was observed . - pubmed
Changes in serum hydroxyproline , hydroxylysine and collagen - like protein levels , and collagen peptidase activity were observed in clinical investigations . - pubmed
Effect of intravenous digitoxin on inotropy , haemodynamics and P - Q interval in ischaemic heart disease . - pubmed
Supernatant fractions ( 100,000 X g ) from isolated nuclei are enriched in complexes containing histones H3 and H4 , which are of distinct size ( 5.6S by sucrose gradient centrifugation , approximate molecular weight of 270,000 by gel filtration ) and negatively charged ( isoelectric at pH 4.4 ) . - pubmed
These complexes bind to DEAE - Sephacel and can be separated from nucleoplasmin . - pubmed
Bacitracin inhibited degradation in both compartments and , although not eliminating insulin trafficking , may impair the transfer of insulin from endosomes to lysosomes . - pubmed
The chimeric PrP , designated MHu2MPrP , differs from MoPrP by 9 amino acids between residues 96 and 167 . - pubmed
Glycation of albumin : reaction with glucose , fructose , galactose , ribose or glyceraldehyde measured using four methods . - pubmed
Albumin was glycated ( nonenzymatically glycosylated ) with glucose , fructose , galactose , ribose or glyceraldehyde for 5 , 9 , 15 and 19 days . - pubmed
The present study determined the effect of genetic obesity and phenobarbital ( PB ) treatment on the expression and regulation of the hepatic cytochrome P450 enzyme ( CYP2C11 ) in Fa/ ? - pubmed
Treatment with PB further suppressed hepatic CYP2C11 protein levels and activities in both fa / fa and Fa/ ? - pubmed
In animal studies , acute interruption of the activity of renal alpha-1 adrenoceptors by renal denervation results in an increase in sodium and water excretion . - pubmed
Chronic selective blockade of alpha-1 adrenoceptors by prazosin in clinical practice has been associated with sodium retention , however . - pubmed
, hour 3 , 7.8 + /- .7 versus 6.3 + /- 2.0 mL / min ; P & lt ; or = .05 ) and fractional excretion of sodium ( e.g. - pubmed
Although EAAs can acutely stimulate the neurocircuitry that controls LH - releasing hormone secretion , the present findings suggest that EAAs might also exert a long - term stimulatory action by acting further upstream in the photoneuroendocrine pathway . - pubmed
Two types of ChAT - immunoreactive synaptic profiles were observed within the ZL and upper SGL using the electron microscope . - pubmed
Suramin induces deoligomerization of human tumor necrosis factor alpha . - pubmed
Suramin inhibits the biological activity of human tumor necrosis factor alpha ( TNF ) through a direct action on the ligand rather than on its receptors ( Grazioli , L. , Alzani , R. , Ciomei , M. , Mariani , M. , Restivo , A. , Cozzi , E. , and Marcucci , F. ( 1992 ) Int . - pubmed
In order to clarify the mechanism whereby suramin leads to inhibition of TNF , we investigated the possibility that suramin might modify the quaternary structure of TNF which is biologically active as a trimer . - pubmed
For this purpose we used a new assay ( double streptavidin sandwich assay ) designed for the rapid detection of oligomer - monomer conversion of proteins . - pubmed
Taking advantage of this assay we observed , upon incubation with suramin , dissociation of TNF . - pubmed
Suramin - induced dissociation of TNF was confirmed by gel filtration chromatography . - pubmed
We conclude that this familial AD may originate from the missense mutation 717Val -- > Ile in the amyloid precursor protein gene and that the clinical picture is typical of AD , except for normal - pressure hydrocephalus and psychiatric phenomena . - pubmed
Merbarone is a catalytic inhibitor of DNA topoisomerase ( topo ) II that does not stabilize DNA - topo II cleavable complexes . - pubmed
Although the cytotoxicity of and resistance to complex - stabilizing topo II inhibitors , such as etoposide , is thought to be mediated through stabilization of these complexes , the mechanisms of cytotoxicity and resistance to catalytic inhibitors are not well known . - pubmed
These novel cell lines are cross - resistant to complex - stabilizing topo II inhibitors , etoposide , teniposide , amsacrine , and doxorubicin , but not to other catalytic inhibitors , aclarubicin or SN-22995 . - pubmed
Of considerable interest , these cell lines are cross - resistant to SN-38 , a putative topo I inhibitor , but cross - resistance to other topo I inhibitors ( camptothecin and topotecan ) was lower and not seen in every cell line . - pubmed
By contrast , there was a low correlation between merbarone and etoposide and between SN-38 and other topo I inhibitors . - pubmed
We propose the hypothesis that decreased topo IIalpha may play a role in the resistance to merbarone that is different from that to complex - stabilizing drugs . - pubmed
We also found that DNA - protein complexes stabilized by SN-38 might be different from those stabilized by topo II inhibitors and blocked by merbarone . - pubmed
Morphine analgesia elicited from the PAG is respectively decreased by selective serotonergic and opioid receptor antagonists administered into the RVM , and increased by RVM neurotensin antagonists . - pubmed
Since glutamate and excitatory amino acid ( EAA ) receptors are also active in the RVM , the present study evaluated whether either competitive ( AP7 ) or non - competitive ( MK-801 ) N - methyl - D - aspartate ( NMDA ) antagonists or a kainate / AMPA ( CNQX ) antagonist microinjected into the RVM altered morphine ( 2.5 micrograms ) analgesia elicited from the PAG as measured by the tail - flick and jump tests . - pubmed
A comparison of this messenger site with the S15 ribosomal binding site was conducted by analysing hydroxyl radical footprintings of these two sites . - pubmed
It has several functions : firm and compliant structural sustenance of capillaries , regulation of the ultrafiltration coefficient by means of the capillary surface area control , phagocytosis and removal of macromolecules and immune complexes , hormone secretion and production of bioactive substances ( endothelin , prostaglandins , renin , cytokines , immunomodulatory peptides , components of the extracellular matrix etc . ) - pubmed
As previously observed , inhibition of AC activity by diazepam was biphasic , with the first phase being receptor - mediated , whereas the second phase involves a direct action on the enzyme itself . - pubmed
An examination of [ 3H]diazepam binding revealed a significant increase in the density of binding sites in denervated striata , with no change in affinity . - pubmed
A time - dependent increase in [ alpha-32P ] GTP labeling of two distinct striatal proteins with apparent molecular masses of 40 and 45 kDa , suggestive of the alpha subunits of Gi and Gs , respectively , was observed . - pubmed
These data indicate that the sensitization of the receptor - mediated inhibitory effect of diazepam on AC activity in denervated striata may involve up - regulation of BZ receptors as well as enhanced functional coupling of these receptors to inhibitory G proteins . - pubmed
The infant was additionally investigated by whole - body scintigraphy with 99mTc - labeled red blood cells to determine the possibility of coexistence of other visceral hemangiomas and by MR , in which the tumor manifested as multiple well - circumscribed space - occupying nodules of high signal intensity on T2 - weighted images with evidence of fast flow . - pubmed
When vesicles composed of an equimolar mixture of sphingomyelin , phosphatidylcholine , phosphatidylethanolamine , and cholesterol are treated with phospholipase C , phospholipid hydrolysis occurs without major changes in vesicle architecture . - pubmed
In the same way , addition of sphingomyelinase leads only to sphingomyelin cleavage . - pubmed
The contribution of both enzymes is unequal , the main role of sphingomyelinase being the production of relatively large amounts of ceramide that will facilitate the lamellar - to - nonlamellar transition in the formation of the fusion pore , whereas phospholipase C provides mainly a localized , asymmetric , high concentration of diacylglycerol that constitutes the trigger for the fusion process . - pubmed
GTP cyclohydrolase I controls the de novo biosynthetic pathway of tetrahydrobiopterin and folic acid . - pubmed
Nonphotochemical chlorophyll fluorescence quenching increased with leaf temperature in a manner consistent with inhibited CER and Rubisco activation . - pubmed
Heat stress led to decreased 3 - phosphoglyceric acid content and increased ribulose-1 , 5 - bisphosphate content , which is indicative of inhibited metabolite flow through Rubisco . - pubmed
Conceptual translation of the SRK cDNA revealed that the catalytic domain of SRK was highly homologous to that of p70S6 K , and that the treatment of wortmannin or rapamycin strongly inhibited the phosphorylation and the activation of SRK , as in p70S6K. - pubmed
The CD4 lymphocyte counts did not differ significantly between TB and DMAC patients ( 26 x 10(6)/L in TB patients vs 5 x 10(6)/L in DMAC patients ) . - pubmed
The chiral labeled cystine ( ( 2 ) H ( beta2 )- cystine ) was prepared by selective hydrogen exchange catalyzed by cystathionine gamma - synthase . - pubmed
Treatment of either cell line with the methyltransferase inhibitor adenosine dialdehyde dramatically shifts hnRNP A2 localization from the nuclear to the cytoplasmic compartment , as shown both by immunoblotting and by immunocytochemistry . - pubmed
In vitro radiolabeling with [ ( 3 ) H ] AdoMet of GST - tagged hnRNP A2 RGG mutants , using recombinant protein arginine methyltransferase ( PRMT1 ) , shows ( i ) that hnRNP A2 is a substrate for PRMT1 and ( ii ) that methylated residues are found only in the RGG domain . - pubmed
The data suggest that hnRNP A2 may contain a novel nuclear localization sequence , regulated by arginine methylation , that lies in the R191 - G253 region and may function independently of the M9 transportin-1 - binding region in hnRNP A2 . - pubmed
This study was aimed at determining whether disruption of the Golgi - vesicular traffic by brefeldin A would change the acetylcholine release from these cells and affect proteins involved in transmitter release like the 15 kDa proteolipid , common to V - ATPase and mediatophore . - pubmed
Physiological characterization showed that rep1 mutations greatly reduced a subset of phytochrome A - regulated responses , including the inhibition of hypocotyl elongation , cotyledon expansion , modulation of gravitropic growth of hypocotyl , and induction of the CAB ( encoding chlorophyll a / b binding protein ) gene , without affecting the accumulation of anthocyanin , far - red - preconditioned blocking of greening , induction of germination , and induction of CHS ( encoding chalcone synthase ) and FNR ( encoding ferredoxin - NADP (+ ) oxidoreductase ) genes . - pubmed
Molecular cloning and characterization of the REP1 gene revealed that it encodes a light - inducible , putative transcription factor containing the basic helix - loop - helix motif . - pubmed
Citrate accumulation and secretion are physiological functions of the prostate gland that are regulated by testosterone and prolactin . - pubmed
The metabolic pathway for citrate production in the prostate involves the activity of mitochondrial aspartate aminotransferase ( mAAT ) . - pubmed
To measure acetylcholine release , the cells on the coverslips were pre - incubated with [ 3H ] choline ( 0.1 micromol / L ) , superfused with modified Krebs - Henseleit buffer at 25 degrees C and electrically stimulated twice for 2 min ( S1 , S2 ; 3 Hz , 0.5 ms , 90 - 100 mA ) . - pubmed
Anti - phosphotyrosine fluorescence intensity in muscle cells of arterioles maintained at 100 mmHg was reduced by the tyrosine kinase inhibitor tyrphostin A47 ( 30 microM ) and increased by the tyrosine phosphatase inhibitor pervanadate ( 100 microM ) . - pubmed
A mutagenesis of the chromosomal glnD gene was carried out by insertion of an interposon carrying the kanamycin resistance gene of Tn5 . - pubmed
Amidated and glycine - extended gastrins were detected with specific antisera and their structures determined by mass spectrometry . - pubmed
These data suggest that two enzymes process the amino terminus of gastrin-17 . - pubmed
Pyrrolidone carboxylic acid peptidase removes the amino terminal pyrrolidone carboxylic acid ( pyroGlu ) , forming gastrin-16 . - pubmed
Glycine - extended forms of gastrin-16 and gastrin-17 comprises 45 % of the total gastrin immunoreactivity . - pubmed
Increases of 24 - h SBP and 24 - h PP after salt loading correlated directly with changes in ADMA ( partial R2 = 0.16 for 24 - h SBP and 0.17 for 24 - h PP , P & lt ; 0.05 for both ) and inversely with changes in EDD ( partial R2 = 0.13 , P = 0.09 for 24 h SBP and partial R2 = 0.15 , P = 0.07 for 24 - h PP ) , after adjustment for age and cholesterol . - pubmed
The key genes nahAc and xylE of the naphthalene catabolism of fluorescent Pseudomonas spp . - pubmed
Catalytic properties of D - amino acid oxidase in cephalosporin C bioconversion : a comparison between proteins from different sources . - pubmed
Lacking an efficient process to produce 7 - aminocephalosporanic acid from cephalosporin C in a single step , d - amino acid oxidase ( DAAO ) is of foremost importance in the industrial , two - step process used for this purpose . - pubmed
gracilis enzyme when the bioconversion is carried out at saturating oxygen concentration ) . - pubmed
We also provide evidence that relief of the negative effect of the AML1 sites is important for Ca ( 2 + ) activation of the GM - CSF promoter / enhancer and that AML1 overexpression increases this Ca ( 2 + ) activation . - pubmed
Purified ATM was phosphorylated on serine 1981 and was active towards a variety of known ATM substrates , including p53 and the Bloom Syndrome helicase , BLM . - pubmed
The protein kinase activity of ATM was selectively inhibited by wortmannin , caffeine and LY294002 and was stimulated by charged biological polymers , including single - stranded M13 DNA ( ssDNA ) , sheared double - stranded calf thymus DNA , heparin sulfate and poly ADP - ribose ( PAR ) , raising the possibility that charged structures may contribute to regulation of ATM activity . - pubmed
However , chemical inhibition of the formation of poly ADP - ribose in cells had no effect on the activation of ATM - dependent pathways by ionizing radiation . - pubmed
Moreover , dephosphorylation of serine 1981 did not affect the apparent molecular weight of ATM in irradiated extracts . - pubmed
Our results suggest that phosphorylation of serine 1981 alone may not directly regulate the subunit composition of ATM . - pubmed
Women carrying BRCA1 and BRCA2 mutations face difficult and confusing reproductive decisions that fall into three categories : issues relating to risk - reducing surgeries , issues relating to use of oral contraceptives / tubal ligation , and issues relating to pregnancy and breastfeeding . - pubmed
Oral contraceptives potentially protect mutation carriers against ovarian cancer but increase the risk of early - onset breast cancer , and evidence for the efficacy of tubal ligation in reducing ovarian cancer risk in BRCA mutation carriers is contradictory . - pubmed
A DFT study of the mechanism of Ni superoxide dismutase ( NiSOD ): role of the active site cysteine-6 residue in the oxidative half - reaction . - pubmed
From the Ni ( III )- O2H species , H2O2 formation occurs through a proton donation by His1 via Tyr9 , which relative to the resting position of the enzyme is exothermic by 4.9 kcal / mol . - pubmed
On the other hand , however , the geometry of the base pair between 5 - methylaminomethyluridine and G ( III ) in a crystal of the ribosomal 30S subunit was quite different from the predicted one [ Murphy IV et al . - pubmed
Salicylic acid ( SA ) participates in the local and systemic response , but SAR does not require long - distance translocation of SA . - pubmed
SAR can be mimicked by foliar JA application and is abrogated in mutants impaired in jasmonate synthesis or response . - pubmed
One of these shRNAs strongly protected cells from tert - BHP and H ( 2 ) O ( 2 ) by specifically reducing the expression of retinol saturase , an enzyme that converts all - trans - retinol ( vitamin A ) to all - trans-13,14 - dihydroretinol . - pubmed
Under basal conditions , perilipin restricts the access of cytosolic lipases to lipid droplets and thus promotes triacylglycerol storage . - pubmed
Quantitative analyses by LC / MS in multiple reaction monitoring mode revealed that resveratrol diglucoside and cis - resveratrol acetyhexoside accumulated up to 2.79 and 10.38 microg / g , respectively , while trans - resveratrol acetylhexoside was barely detectable . - pubmed
To begin to understand the mechanisms for the development of acute insulin resistance , serine phosphorylation of insulin receptor substrate ( IRS )- 1 and c - Jun N - terminal kinase phosphorylation / activation was investigated . - pubmed
This study was performed to determine whether dexamethasone ( DEX ) had an effect on calcium - activated potassium channels ( K(Ca ) channels ) and Occludin protein in blood - brain tumor barrier ( BTB ) . - pubmed
We compare the adsorption behavior of wild and surface - modified ferritin on a homopolymer blend of poly ( desaminotyrosyl tyrosine dodecyl ester carbonate ) ( PDTD ) and poly ( epsilon - caprolactone ) ( PCL ) . - pubmed
Wild ferritin was alkylated by zero length cross - linking to surface carboxylate groups activated by water soluble carbodiimide ( EDC ) . - pubmed
Transmission electron microscopy shows a clear selectivity of alkylated ferritin adsorption onto the PCL phase while wild ferritin predominantly adsorbs onto the PDTD phase . - pubmed
However , we demonstrate that PKR and eIF2alpha phosphorylation at serine 51 are not downstream of p53 in response to doxorubicin treatment suggesting that PKR is not a universal target of p53 as proposed . - pubmed
However , several recent developments have seriously threatened the prominence of this drug class : ( i ) the adoption of an early invasive strategy , frequently leading to percutaneous coronary intervention ( PCI ) where the dosing and monitoring of LMWH is unfamiliar to most operators , ( ii ) the results of the SYNERGY trial , which not only failed to establish the superiority of enoxaparin over unfractionated heparin with respect to efficacy , but also demonstrated more bleeding with LMWH , and ( iii ) the results of the REPLACE-2 and ACUITY trials , which have demonstrated the advantages of an ACS and PCI treatment strategy based on direct thrombin inhibition with bivalirudin . - pubmed
A key concept that is presented is that the ACT is a more accurate measure of LMWH - induced anticoagulation than of UFH - induced anticoagulation . - pubmed
These guidelines may facilitate continued use of LMWH , which has the potential to reduce cost ( less expensive than bivalirudin ) , diminish the need for intravenous medication ( can be administered subcutaneously in the noninvasive setting with minimal to no monitoring ) , and provide an ideal anticoagulant during PCI ( easy to monitor with the ACT , at least partially reversible with protamine in the event of coronary perforation , effective antithrombin with no platelet activation , thereby potentially reducing the need for routine adjunctive IIb / IIIa inhibition ) . - pubmed
TBT shifts the Th1 / Th2 balance to Th2 through reduced IL-12 production by dendritic cells and augmentation of IL-10 production by B cells , indicating that TBT contributes to the increase in the number of allergic diseases . - pubmed
Given that azanucleosides affect human leukocyte antigen expression and lymphocyte reactivity , we conducted a retrospective study to define the impact of pre - HCT azanucleoside therapy on post - HCT donor chimerism . - pubmed
DUSP1 / MKP-1 is implicated in cellular protection against a variety of genotoxic insults including hydrogen peroxide , ionizing radiation , and cisplatin , but its role in the interplay between different MAPK pathways in determining cell death and survival is not fully understood . - pubmed
The one - pot difunctionalization of dibromopyridines was also realized using the reagent stoichiometrically . - pubmed
The lectin haemagglutinating activity was optimum between pH 8.0 and 9.0 and inhibited after incubation with D - galactose and its derivatives , especially alpha - methyl - D - galactopyranoside . - pubmed
We noted no apparent interaction of 1,25 ( OH )( 2 ) D ( 3 ) with epidermal growth factor , insulin - like growth factor , cholera toxin , or transforming growth factor - beta , but we did find synergistic inhibitory effects of 1,25 ( OH )( 2 ) D ( 3 ) with suramin and retinoic acid . - pubmed
Using computer simulations to determine the limitations of dynamic clamp stimuli applied at the soma in mimicking distributed conductance sources . - pubmed
The data from 3 -( 4,5 - dimethylthiazol-2 - yl )- 2,5 - diphenyltetrazolium bromide test , Hoechst33342 staining , terminal deoxynucleotidyl transferase dUTP - mediated nicked end labeling assay , and Bax / Bcl-2 ratio analysis indicated that salidroside pretreatment attenuated hypoxia - induced apoptotic cell death of primary cultured cortical neurons in a dose - dependent manner . - pubmed
Moreover , preliminary exploration of the possible mechanisms suggested that the protective effects of salidroside , shown in our experimental setting , might probably be mediated by enhancing the expression of hypoxia - inducible factor-1alpha , alleviating the increase of intracellular reactive oxygen species levels , and inhibiting over - expression of nuclear factor - kappa B protein . - pubmed
Homology modeling of mosquito cytochrome P450 enzymes involved in pyrethroid metabolism : insights into differences in substrate selectivity . - pubmed
The CYP6AA3 model has a large active site allowing it to accommodate multiple conformations of pyrethroids . - pubmed
The predicted CYP6P7 active site is more constrained and less accessible to binding of pyrethroids . - pubmed
The absence of CYP6P8 activity toward pyrethroids appears to be due to its small substrate access channel and the presence of R114 and R216 that may prevent access of pyrethroids to the enzyme heme center . - pubmed
Information obtained using homology models has the potential to enhance the understanding of pyrethroid metabolism and detoxification mediated by P450 enzymes . - pubmed
We have identified and synthesized a series of imidazole containing dimerization inhibitors of inducible nitric oxide synthase ( iNOS ) . - pubmed
The SLC45A3 - ELK4 fusion , but not wild - type , ELK4 plays important roles in regulating cell growth in both androgen - dependent and -independent prostate cancer cells . - pubmed
We extend our previous work on the conformation switching of polyzwitterionic brushes grafted onto ECP to the case of ABC random polyampholyte brushes : here , a statistical near - neutral copolymer of methyl methacrylate ( MMA ) , methacryloxyethyltrimethylammonium chloride ( METAC ) , and 3 - sulfopropyl methacrylate potassium salt ( SPMA ) . - pubmed
The behavior can be contrasted with that of zwitterionic brushes , which show a switch between collapsed with ECP oxidized and expanded with ECP reduced , independent of salt concentration over the same range ( 10(-3)-2 M NaCl ) as that studied here , and that of zwitterionic - hydrophobic block copolymers where the switch is suppressed at low salt concentration . - pubmed
What have we learnt about high - density lipoprotein cholesterol measurements during 32 years ? - pubmed
BACKGROUND : High - density lipoprotein cholesterol ( HDL - C ) is important in risk assessment for cardiovascular disease or metabolic syndrome ; however , different direct HDL - C assays may lead to erroneous risk estimates and potentially misclassify people . - pubmed
The formation of a polar pocket involving Asn202 in the lumen of the tetrameric TM bundle is crucial for the assembly and solvation of the domain . - pubmed
Amino acid variation within the P2 domain of the major capsid protein , VP1 , was followed longitudinally within the GII.4 lineage . - pubmed
The configuration at C6 in the 7 - carba analogs does not influence the rate of acid - catalyzed hydrolysis but has a minor influence on the rate of spontaneous hydrolysis of the 2,4 - dinitrophenyl galactosides , confirming earlier studies on the role played by the hydroxymethyl group conformation on glycoside reactivity . - pubmed
Results show that 31 % and 0.5 % of the total aboveground carbon storage in the LS and PB , respectively was located in high forest damage risk ( HR ) zones . - pubmed
RESULTS : Titanium ( Ti ) or polymethylmethacrylate ( PMMA ) particles ( 0.1 mg / mL ) did not significantly change the viability of BMMs , but twice increased the differentiation of BMMs into mature osteoclasts , and markedly increased TNF - alpha production . - pubmed
The TNF - alpha level in the PMMA group was significantly higher than in the Ti group ( 96 h ) . - pubmed
Furthermore , VIVIT reduced TNF - alpha production much more efficiently in the PMMA group than in the Ti group ( 96 h ) . - pubmed
It is concluded that MDP can significantly enhance the NOD2 mRNA and protein expression in sensitized DC , promote the expression of HLA - DR , synergistic costimulatory molecules and adhesion molecules of DC , at the same time , MDP can increase secretion of inflammatory factors IL-12p40 . - pubmed
Hemoglobin A1c ( HbA1c ) is widely used in the general population for diabetes diagnosis and monitoring as it highly correlates with blood glucose levels and outcomes . - pubmed
Autophagy induced by EEF2 K silencing promotes cell survival and does not potentiate the anticancer efficacy of the AKT inhibitor MK-2206 . - pubmed
N - terminal sequencing of the trypsin - activated Cry7Aa fragments revealed that processing occurs at Glu(47 ) for the 70 - kDa form or Ile(88 ) for the 65 - kDa form . - pubmed
Here we report engineered variants of the photoconvertible Eos fluorescent protein that fluoresce and photoconvert normally in heavily fixed ( 0.5 - 1 % OsO4 ) , plastic resin - embedded samples , enabling correlative super - resolution fluorescence imaging and high - quality electron microscopy . - pubmed
Discovery of DS-5272 as a promising candidate : A potent and orally active p53 - MDM2 interaction inhibitor . - pubmed
We have published p53 - MDM2 interaction inhibitors possessing a novel dihydroimidazothiazole scaffold . - pubmed
APCcy and APCox induced a robust T cell BrdU incorporation . - pubmed
Acetylcholine enhances keratocyte proliferation through muscarinic receptor activation . - pubmed
Activation of the MAPK / Erk signaling pathway and its involvement in ACh - enhanced proliferation was determined by western blot analysis , MTS , and BrdU ELISA . - pubmed
Western blot analysis revealed that ACh stimulation of keratocytes upregulated the expression of PCNA and Ki-67 , and Ki-67 immunocytochemistry showed that ACh - treated cells were in an active phase of the cell cycle . - pubmed
ACh activated MAPK signaling , and this step was crucial for the ACh - enhanced proliferation , as inhibition of the MAPK pathway resulted in ACh having no proliferative effect . - pubmed
Accumulating evidence has suggested that DES with cobalt chromium stent platform , modified biodegradable polymer coatings , and rapamycin derivative drugs are associated with improved clinical outcomes . - pubmed
Short - term exposure to PM2.5 and dLAN upregulated neuroinflammatory cytokines and altered CA1 hippocampal structural changes , as well as provoked depressive - like responses ( anhedonia ) . - pubmed
The Vivartana treated CTX induced group shows increase in the hemoglobin level compared with the CTX induced group . - pubmed
Similarly the lowered bone marrow cellularity and number of alpha - esterase positive cells in CTX induced group were restored in the Vivartana treatment . - pubmed
Treatment with vivartana also exhibits hepatoprotective activity by regulating the SGOT and SGPT levels in CTX induced group . - pubmed
The two isoflavones were effective in enhancing the activity of NSAID activated gene ( NAG-1 ) at 2.5 pg / mL ( 1.5 - 1.8 fold increase ) and inhibiting iNOS and NF - kappaB activity with IC50 values in the range of 6 - 13 microg / mL. - pubmed
As observed by transmission electron microscopy ( TEM ) , MPE , OSA and POM also inhibited arachidonic acid - induced tau fibrillization in a concentration - dependent manner . - pubmed
Meanwhile , the changes of LC3 - II and P62 levels and formation of autophagosome suggested that resveratrol in combination with cisplatin triggered autophagy . - pubmed
Significant RAS activates the renin - angiotensin - aldosterone system and is associated with three major clinical syndromes : ischemic nephropathy , hypertension , and destabilizing cardiac syndromes . - pubmed
The longitudinal and transverse axes of 4 experimental groups ( Tic1 , Tic2 , Zrc1 , and Zrc2 ) were analyzed ( vertical / horizontal adjustment ) using a scanning electron microscope at x1000 magnification . - pubmed
Acetazolamide enhances the release of urinary exosomal aquaporin-1 . - pubmed
A previous study has demonstrated that acetazolamide ( AZ ) , a diuretic that inhibits carbonic anhydrases , alters the expression level of AQP1 in cultured cells . - pubmed
Treatment with furosemide did not change , whereas NaHCO3 and NH4Cl decreased the exosomal release of AQP1 . - pubmed
Anti - Ro60 were purified by elution from native Ro60 - coated ELISA plates and subjected to combined de novo amino acid sequencing and database matching . - pubmed
Specific IgV amino acid substitutions stratified anti - Ro60 from anti - Ro60 / La responses , providing a molecular fingerprint of Ro60 / La determinant spreading and suggesting that different forms of Ro60 antigen drive these responses . - pubmed
After 8 - week treatment , the increase in roughness caused by 16 % CP was significantly higher ( p & lt ; 0.05 ) than the one caused by 40 % H2O2 . - pubmed
Bleaching with CP 16 % resulted in higher roughness than bleaching with H2O2 , while 40 % H2O2 caused the higher microhardness increase . - pubmed
Effects of endurance exercise and doxorubicin on skeletal muscle myogenic regulatory factor expression . - pubmed
Endurance exercise enhances MRF expression and thereby may mitigate DOX - induced MRF disruptions . - pubmed
This study examined the effects of endurance training and DOX treatment on myogenic regulatory factor ( MRF ) expression . - pubmed
Results : Significant Myf5 drug and activity effects were observed in the soleus with EXER+DOX expressing higher Myf5 than SED+DOX . - pubmed
No main effects or interactions were observed in the EDL , but in the diaphragm , a significant activity effect was observed for Myf5 with EXER+DOX expressing higher levels than SED+DOX . - pubmed
D - Dimer Levels and Effect of Rivaroxaban on Those Levels and Outcomes in Patients With Acute Coronary Syndrome ( An ATLAS ACS - TIMI 46 Trial Substudy ) . - pubmed
It was hypothesized that elevated baseline D - dimer would be associated with poor clinical outcomes in ACS , and that Factor Xa inhibition with Rivaroxaban would reduce D - dimer acutely and chronically . - pubmed
In conclusion , elevated baseline D - dimer was associated with an increased risk of the composite outcome within 6 months of the ACS event and administration of the Factor Xa inhibitor rivaroxaban was associated with lower D - dimer levels compared with placebo after the first dose , at day 30 and day 180 . - pubmed
Sunitinib is an orally administered tyrosine kinase inhibitor . - pubmed
The aim of the present study was to examine the light stability of sunitinib and confirm the effects of light exposure on sunitinib measurements by LC - MS / MS . - pubmed
Ribosome - inactivating proteins ( RIPs ) are enzymes with N - glycosylase activity that remove adenine bases from the ribosomal RNA . - pubmed
It is noteworthy that this is the first study presenting the complete amino acid sequence of a RIP from a Gypsophila species . - pubmed
The full amino acid sequence of gypsophilin - S was assembled by MALDI - TOF - MS - MS and PCR . - pubmed
The isophthalate - induced proteins include a phenylacetate : CoA ligase , a UbiD - like decarboxylase , a UbiX - like flavin prenyltransferase , and a hypothetical protein . - pubmed
Subsequently , benzoyl - CoA is metabolized by the enzymes of the anaerobic benzoate degradation pathway that were identified in the proteomic analysis . - pubmed
In vitro enzyme assays with cell - free extracts of isophthalate - grown cells indicated that isophthalate is activated to isophthalyl - CoA by an ATP - dependent isophthalate : CoA ligase ( IPCL ) , and subsequently decarboxylated to benzoyl - CoA by a UbiD family isophthalyl - CoA decarboxylase ( IPCD ) that requires a prenylated flavin mononucleotide ( prFMN ) cofactor supplied by UbiX to effect decarboxylation . - pubmed
Homologs of the IPCD encoding genes are found in several other bacteria , such as aromatic compound - degrading denitrifiers , marine sulfate - reducers , and methanogenic communities in a terephthalate - degrading reactor . - pubmed
Both PQQ-42 and PQQ-44 were found to degrade a wide range of N - acylhomoserine lactone ( AHL ) QS signals , possibly due to the presence of an aac gene which encodes an AHL amidohydrolase . - pubmed
The Correlation between Malondialdehyde and Nerve Growth Factor Serum Level with Diabetic Peripheral Neuropathy Score . - pubmed
AIM : This study was conducted to identify malondialdehyde ( MDA ) serum level , nerve growth factor ( NGF ) serum level , diabetic peripheral neuropathy score and the correlation between MDA and NGF serum level with diabetic peripheral neuropathy score . - pubmed
There were significant correlations between the MDA serum level and the diabetic peripheral neuropathy score ( r = 0.364 , p = 0.048 ) , and between the NGF serum level with the diabetic peripheral neuropathy score ( r = -0.59 , p = 0.001 ) . - pubmed
Low NGF serum level plays a bigger role than high MDA serum level in diabetic peripheral neuropathy . - pubmed
BaB2 O3 F2 : A Barium Fluorooxoborate with a Unique 2 [ B2 O3 F]- Layer and Short Cutoff Edge . - pubmed
BaB2 O3 F2 exhibits a unique oxyfluoride layer 2 [ B2 O3 F]- and a deep - ultraviolet cutoff edge below 180 nm . - pubmed
Octreotide - LAR in later - stage autosomal dominant polycystic kidney disease ( ALADIN 2 ): A randomized , double - blind , placebo - controlled , multicenter trial . - pubmed
This review summarizes novel imaging in the management of prostate cancer including multiparametric MRI , PET - CT scans with different radiotracers including 11C - acetate , 11C - choline , 18F - choline , 18F sodium fluoride , prostate - specific membrane antigen , and anti-1 - amino-3 -[ 18F ] fluorocyclobutane-1 - carboxylic acid ( fluciclovine ) . - pubmed
The PCSK9i that are currently licensed for clinical use , evolocumab and alirocumab , have shown high potency in lowering LDL - cholesterol , which can exceed 60 % , and other favourable effects on lipid profiles , including a very marked reduction of non - HDL cholesterol and apolipoproteinB. - pubmed
CONCLUSION : Nonstudy oral PrEP use in the first phase 2 study of tenofovir reduced - glycerin 1 % gel was high at a single site in SF where community PrEP availability and use was expanding . - pubmed
The VPP can be used to evaluate automated insulin and glucagon delivery algorithms , so - called artificial pancreas ( AP ) algorithms that are currently being used to help people with T1D better manage their glucose levels . - pubmed
Consequently , persisting adaptive changes may occur in the glucose - insulin metabolism , including reduced capacity for insulin secretion and insulin resistance . - pubmed
Doxorubicin conjugated AuNP / biopolymer composites facilitate cell cycle regulation and exhibit superior tumor suppression potential in KRAS mutant colorectal cancer . - pubmed
The degradation of physiological sAbeta was prevented by a highly selective insulin - degrading enzyme inhibitor ( Ii1 ) but not by a phagocytosis inhibitor ( polyinosinic acid ) or pinocytosis inhibitor ( cytochalasin B ) in vitro . - pubmed
Materials and Methods : Here , in our designed synthesis route , the traditional FeCl3 precursor was replaced with Fe2O3 nanospheres , and then the Prussian blue ( PB ) nanoparticles were engineered into hollow - structured PB ( HPB ) nanospheres through an interface reaction , where the Fe2O3 colloidal template provides Fe3 + ions . - pubmed
Meanwhile , HPB nanospheres also demonstrated a high DOX loading capacity of 440 mg g-1 as a drug carrier , and the release of the drug can be regulated by the heat from PB shell under the exposure of an NIR laser . - pubmed
Blood tests were executed to assess the blood glucose and lipid indicators , including HbA1c , fasting plasma glucose ( FPG ) , triglyceride ( TG ) , total cholesterol ( TC ) , low - density lipoprotein cholesterol ( LDL - C ) , high - density lipoprotein cholesterol ( HDL - C ) , apolipoprotein B100 ( apoB100 ) , prebeta1 - HDL , HDL3b , apoB100 / AI , apolipoprotein AI ( apoAI ) , HDL2b and HDL2a . - pubmed
Subsequently , four decades later , Ingram and colleagues characterized the A to T substitution in DNA producing the GAG to GTG codon and replacement of glutamic acid with valine in the sixth position of the betaS - globin chain . - pubmed
In this mini review , we will critically review previous and ongoing clinical trials of anti - CD20 monoclonal antibodies in MS , including rituximab , ocrelizumab , ofatumumab , and ublituximab . - pubmed
Studies on calcium ion - induced conformation changes in the actin - tropomyosin - troponin system by fluorimetry . - pubmed
Conformational changes in the region containing fluorescence - labeled sulfhydryl group(s ) of troponin . - pubmed
Conformational changes associated with the functional states of the molecule of troponin were studied using SH - direct fluorogenic reagents , N -( p -( 2 - benzimidazolyl ) phenyl ) maleimide ( BIPM ) and N -( 1 - anilinonaphthyl-4 ) maleimide ( ANM ) . - pubmed
On addition of Ca2 + , the fluorescence characteristics of the two systems , ANM - troponin . - pubmed
When BIPM was used in place of ANM , similar changes were observed : a simple decrease in the intensity when pCa was decreased from 7.4 to 5.5 in the system without F - actin and a sigmoidal increase in the range from pCa 7 to 6 in the system with F - actin . - pubmed
Heavy meromyosin , when added to the latter complex ( the reconstituted thin filaments ) , made the profile of its Ca2 + concentration dependence of fluorescence similar to that of the former complex . - pubmed
The data obtained imply that the Ca2 + -induced conformational changes of troponin are markedly modified when detached from actin , and that heavy meromyosin weakens the interaction of the troponin . - pubmed
A phosphodiesterase inhibitor , diazoxide ( Dx ) , caused insignificant increase in the basal cAMP , without affecting the force of contraction or CF . - pubmed
The activity of this enzyme was manifested mainly in the conditions of continuous cultivation at a rate of 0.1 - -0.2 hr-1 and a final nitrogen concentration of 0.006 - -0.025 g per litre of the medium . - pubmed
If the concentration of nitrogen was increased in these conditions , biosynthesis of L - glutaminase - asparaginase I was stimulated while the activity of L - glutaminase - asparaginase II was not detected at all . - pubmed
Under conditions favourable for phospholipase - induced mitochondrial swelling on account of phospholipase activation ( low Mg2 + concentration , and Ca2 + present in the incubation medium ) the hormone proved to accelerate the swelling of the organellae . - pubmed
When phospholipase - induced mechanism of mitochondrial swelling was inhibited ( high Mg2 + concentration ) thyroxin depressed considerably the swelling of organellae induced by lipid peroxidation due to its antioxidant action . - pubmed
The extent of the inhibitory effect of ricin in polyphenylalanine synthesis by eukaryotic ribosomes is strongly dependent upon the concentration of ribosomes and the elongation factors EF 1 and EF2 . - pubmed
Similarly , the enzymatic binding of Phe - tRNA to ribosomes is drastically blocked in ricin - treated ribosomes when low EF 1 concentrations are added to the reaction mixture , but there is no inhibition when EF 1 is at saturating concentrations . - pubmed
Furthermore , formation of the complex EF 2 - guanosine triphosphate - ribosome , using free ribosomes pretreated with ricin , is strongly inhibited at limiting concentrations of EF2 , but is not affected at saturating concentrations of this factor . - pubmed
However , ricin does not inhibit the EF 2 - dependent translocation of peptidyl - tRNA by polysomes , although the toxin is very active in preventing amino acid incorporation by polysomes . - pubmed
Maximal expression of Ia antigen and release of interleukin 1 ( IL1 ) were observed during the first week of pregnancy ( implantation ) , when the highest peak of estradiol was produced . - pubmed
Although PGE2 production had no evident relation with Ia antigen expression and IL1 activity during the first and second weeks of pregnancy , the increment in PGE2 values and percentages of NBT+ cells could indicate a different stage of macrophage activation . - pubmed
Insulin - like growth factor I modulates voltage - dependent Ca2 + channels in neuronal cells . - pubmed
The effect of IGF - I occurred after 1 - 2 h incubation and reversed within 24 h. Ca2 + channel currents recorded in control cells were mainly of a low - threshold fast inactivating type and showed a mean density of 5.9 + /- 0.3 pA / pF. - pubmed
Ca2 + channel currents in cells treated with IGF - I showed an enhanced amount of a high - threshold slowly inactivating Ca2 + current type sensitive to the dihydropyridine isradipine and the snail toxin omega - conotoxin . - pubmed
The effect of IGF - I was suppressed by coincubation with the PKC inhibitors 1 -( 5 - isoquinolinylsulfonyl )- 2 - methyl - piperazine ( H-7 ) and staurosporin which were both without effect on current density in control cells . - pubmed
The effects of IGF - I and phorbol ester were not additive . - pubmed
Post - aSNA responded with a tendency to increase after the 2 - DG injection . - pubmed
The increase in adipocyte differentiation in response to Dex was associated with a decrease in c - fos and actin RNA expression whereas the decrease in c - fos RNA expression in response to Dex was small ( approximately 40 % ) ; immunocytochemical analysis indicated that induction of Fos protein occurred only in undifferentiated cells . - pubmed
Expression of IGF - I RNA and secretion of IGF - I and PGE2 were also decreased in response to Dex treatment . - pubmed
Deletion of the carboxyl - terminal tail domain of either protein indicated that this region was not absolutely essential for co - assembly into the endogenous vimentin cytoskeleton . - pubmed
Thus , an intact alpha - helical rod domain was essential for normal IF co - assembly whereas carboxyl - terminal deletions into this region resulted in dramatic alterations of the existing type III and IV intermediate filament cytoskeletons in vivo . - pubmed
The interaction between EF - Tu and mitochondrial Phe - tRNA was investigated by using the ability of EF - Tu to protect the aminoacyl - tRNA bond from hydrolysis . - pubmed
coli EF - Tus are capable of interacting with mitochondrial Phe - tRNA . - pubmed
However , ribosomal A - site binding assays demonstrated that efficient binding of the mitochondrial Phe - tRNA to the ribosomal A - site was only obtained with the homologous mitochondrial EF - Tu . - pubmed
Serum immunoglobulins ( IgG , IgA , IgM ) were measured as well as total and active E - RFC and cellular immunity was evaluated using the intradermal testing of PPD , candidin D-7 , trichophytin D-5 and tetanus anatoxin . - pubmed
Early afterdepolarizations and delayed afterdepolarizations can both be suppressed by low external Ca2 + concentrations or by Ca2 + channel blockers . - pubmed
Comparison of proline transport and oxygen uptake by membrane vesicles of the wild - type transductant and the mutant ( ttr-3 ) transductant showed that membrane vesicles of the mutant exhibited temperature - sensitive decrease of proline transport and increase of oxygen uptake at the restrictive temperature ( 42 degrees C ) , compatible with depression of growth of the mutant at this temperature . - pubmed
Significant interaction between low - spin iron ( III ) site and pyrroloquinoline quinone in active center of nitrile hydratase . - pubmed
Nitrile hydratase , a unique non - heme iron enzyme , contains low - spin Fe ( III ) site and pyrroloquinoline quinone in the active center . - pubmed
The enzymatic activity of nitrile hydratase is strongly inhibited by typical carbonyl reagents such as phenylhydrazine and semicarbazide . - pubmed
Of special interest is the fact that these carbonyl reagents induced significant alteration of the ESR features of the native iron ( III ) enzyme at pH 7.2 . - pubmed
In addition , the isobutyronitrile ( inhibitor )- bound enzyme was also remarkably affected by phenylhydrazine and then the transformed ESR characteristics were different from those of phenylhydrazine - treated enzyme . - pubmed
The present results reveal the first evidence for an important interaction between the low - spin Fe ( III ) site and pyrroloquinoline quinone which is essential for the activity of nitrile hydratase . - pubmed
Molecular biology of gelsolin , a calcium - regulated actin filament severing protein . - pubmed
In the presence of micromolar Ca2 + , gelsolin severs actin filaments , causing profound changes in the consistency of actin polymer networks . - pubmed
A variant of gelsolin containing a 25 - amino acid extension at the NH2 - terminus is present in plasma where it may be involved in the clearance of actin filaments released during tissue damage . - pubmed
The NH2 - terminal half of the molecule contains a Ca2 + -insensitive actin severing domain while the COOH - terminal half contains a Ca2 + -sensitive actin binding domain which does not sever filaments . - pubmed
These data suggest that the NH2 - terminal severing domain in intact gelsolin is influenced by the Ca2 + -regulated COOH - terminal half of the molecule . - pubmed
These cells are known to respond to estradiol by increasing progesterone receptor levels , alkaline phosphatase activity , and cell density . - pubmed
Nuclease S1 was used to recognize and cleave regions made permanently single - stranded due to osmium recognize and cleave regions made permanently single - stranded due to osmium modification . - pubmed
In linearized plasmids treatment with Os , py produced no S1 - detectable site - specific modification . - pubmed
This H2O2 - dependent mutagenicity of DCB was prevented by horseradish peroxidase . - pubmed
These results suggest that mechanisms , perhaps enzymes endogenous to tester strains TA98 , may play a role in the activation of DCB . - pubmed
Elimination of hippocampal CA3 and CA4 pyramidal cells with kainic acid ( KA ) induces a performance deficit on a forced - choice alternation task , which recovers over time . - pubmed
The three activities appear to be different since cysteine inhibited casein digestion , aurothiomalate inhibited histone digestion , and neither inhibited proteoglycan digestion . - pubmed
A total of 215 of these were tested for hepatitis B antigen ( HB Ag ) by radioimmunoassay and 32 ( 15 % ) were positive . - pubmed
There is a relatively small , but constant , amount of superoxide released from alveolar macrophages at rest . - pubmed
This syndrome is characterized by excess triglyceride ( TG ) accumulation in the liver and apparent decreased hepatic lipoprotein output . - pubmed
Phytic acid is undigestible by the monogastric but rumen phytases are assumed to adequately hydrolyze it . - pubmed
In above three species the synthesis of longer chain mycolic acids ( longer than C44 or C46 ) was inhibited more significantly than shorter homologues such as C38 or C40 . - pubmed
Furthermore , mass fragmentographic analysis , monitoring fragment ions ( A ) and ( B ) , due to straight chain and branched chain alkyl units , respectively , demonstrated the inhibition of mycolic acids was not attributed to the shortening of alpha - alkyl chain , but to the inhibition of chain elongation of C28 to C32 straight chain meromycolic acids . - pubmed
Furthermore our data demonstrate the presence of thioesterase activity associated with liver , lung , kidney and brain microsomes capable of hydrolysing TTPG and thus liberating free thiol groups . - pubmed
Role of intracellular Ca2 + levels in the regulation of CD11a / CD18 mediated cell adhesion . - pubmed
The results demonstrate that , in addition to protein tyrosine kinase ( PTK ) and protein kinase C ( PKC ) mediated signalling , increase in cytosolic - free calcium ( [ Ca2+]i ) levels play a major role in the activation of LFA-1 . - pubmed
The calcium ionophore Ionomycin , which increases [ Ca2 +]i is capable of directly activating LFA-1 . - pubmed
Furthermore , activation of LFA-1 by triggering through CD2 , CD3 or MHC class II is associated with an increase in [ Ca2 +]i levels , with kinetics that directly correlate with cell adhesiveness . - pubmed
Moreover , entry of extracellular Ca2 + via Ca - channels is involved in both the CD3- and MHC class II , as well as part of the CD2 induced LFA-1 activation . - pubmed
Depletion of intracellular calcium results in unresponsiveness of LFA-1 to these stimuli , further demonstrating a regulatory role for [ Ca2 +]i in LFA-1 mediated adhesion . - pubmed
We also investigated the effects of superoxide dismutase conjugated to polyethylene glycol and catalase , but the number of vomiting episodes and latency did not change significantly when these agents were intraperitoneally injected 30 min prior to or 20 min after the administration of cisplatin . - pubmed
The CKII target sites have been mapped to multiple serine and threonine residues on 4 tryptic fragments within the C - terminal 350 amino acids of yeast topoisomerase II . - pubmed
Because both cytokines and neurohormones may influence the macrophage system , the current study was done to evaluate the effects of IL-3 and of the pineal hormone melatonin ( MLT ) on monocyte response to IL-2 administration . - pubmed
CONCLUSIONS : These results suggest that IL-3 alone or in association with MLT may modulate macrophage functions during cancer immunotherapy with IL-2 and decrease the IL-2 - induced macrophage activation . - pubmed
In order to determine the role of the C - terminal region of DSR - S in dextran or oligosaccharide synthesis , four DSR - S genes with deletions at the 3 ' end were constructed . - pubmed
In fact , it seems that the role of the C - terminal domain of DSR - S may be to facilitate the translation of dextran and oligosaccharides from the catalytic site . - pubmed
Three fractions were recovered : UF was NT non - retained in immobilised AMP ; F - I was bound enzyme eluted with beta - glycerophosphate , and F - II was bound NT released with AMP . - pubmed
eNT was 80000 - fold purified in F - II , this fraction showing proteins of 74 , 68 and 51 kDa after immunoblotting . - pubmed
NT in UF migrated at 6.7S after centrifugation in sucrose gradients with Triton X-100 , the peak being split into two of 6.7S and 4.4S in gradients with Brij 96 . - pubmed
Ecto - NT in F - I or F - II migrated at 5.8S in Triton X-100- , or 4.4S in Brij 96 - containing gradients . - pubmed
A zinc / protein molar ratio of 2.2 was determined for eNT in F - II . - pubmed
NT activity was decreased in assays made in imidazole buffer , and was partly restored with 10 microM Zn2 + or 100 microM Mn2 + . - pubmed
In assays with Tris buffer , NT showed a Km for AMP of 12 microM , and was competitively inhibited by ATP or ADP . - pubmed
A novel mutation at a probable heme - binding ligand in neutrophil cytochrome b558 in atypical X - linked chronic granulomatous disease . - pubmed
A membrane - bound cytochrome b558 , a heterodimer consisting of gp91 - phox and p22 - phox , is a critical component of the superoxide ( O2-)-generating reduced nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase in phagocytes . - pubmed
We report here an atypical case of X - linked CGD in which neutrophils showed a complete absence of O2 - -forming NADPH oxidase activity , but a small amount ( about 10 % of control ) of both subunits was detected by immunoblot analysis . - pubmed
Spectrophotometric studies of the neutrophils with a recently developed sensitive method gave no evidence for the heme spectrum in the cytochrome b558 , of this CGD . - pubmed
Reverse transcription / polymerase chain reaction and sequence analysis revealed a C to T transition replacing histidine at amino acid position 101 ( His101 ) by tyrosine in gp91 - phox . - pubmed
These results provide evidence that His101 of gp91 - phox is the one of the heme - binding ligands of cytochrome b558 . - pubmed
The contractions of normal uterine smooth muscles to oxytocin " 0.1 , 1 and 10 mU " and to ACh " 0.1 , 1 and 10 uM " were significantly reduced in the presence of EAC cells . - pubmed
Metallated species ( Cd , Zn and mixed Cd - Zn complexes ) were decomposed on - line to produce apo metallothioneins of which the molecular masses were determined by MS . - pubmed
Carbachol and endothelin-1 increased GTP - bound p21 ( ras ) in a pertussis toxin - sensitive manner [ ratio of [ 32P ] GTP to ( [ 32P]GTP + [ 32P ] GDP ): control , 30 + /- 1.7 ; 3 min of 1 microM carbachol , 39 + /- 1.1 ; 3 min of 1 microM endothelin-1 , 40 + /- 1.2 ] , whereas histamine , bradykinin , and KCl were without effect . - pubmed
Transfection of an inhibitor of the G protein betagamma - subunit [ the carboxy terminus ( Gly495 - Leu689 ) of the beta - adrenoceptor kinase 1 ] failed to inhibit the carbachol - induced activation of p21 ( ras ) . - pubmed
They are cysteine - rich glycoproteins predominantly made of multiple repeats homologous to the calcium - binding epidermal growth factor module , and are translated as precursor proteins cleaved by furine / PACE - like activities . - pubmed
Ionization of this glutamic acid side chain causes a pH - dependent decrease in the thermal stability of leishmania TIM . - pubmed
The presence of this glutamate , also in its protonated state , disrupts to some extent the conserved hydrogen bond network , as seen in all other TIMs . - pubmed
METHODS : Venous blood samples were drawn every 2 h over 25 h for measurements of atrial natriuretic peptide ( ANP ) , brain natriuretic peptide ( BNP ) , norepinephrine , renin activity , and aldosterone and two urine collections ( 12 h each ) for stable prostaglandin metabolite measurements . - pubmed
Plasma renin activity and aldosterone did not differ between the groups . - pubmed
CONCLUSIONS : We conclude that mild preeclampsia is associated with elevated plasma norepinephrine levels , lower systemic daytime production of prostacyclin , and blunting of the normal diurnal variation for a number of indices including plasma levels of BNP , ANP , norepinephrine , and aldosterone , and urinary prostacyclin metabolites . - pubmed
Recombinant epoetin therapy and correction of the chronic anemia of renal failure have greatly reduced the number of red cell transfusions and hence the propensity to iron overload . - pubmed
Iron overload before the availability of epoetin constituted a serious problem ; our review of the literature does not decisively conclude that these patients had more serious bacterial infections or increased mortality when compared with their non - iron overloaded counterparts , unless chronic transfusion - related hepatic disease was superimposed . - pubmed
Optimized anemia therapy in ESRD requires individualized and specific application of epoetin and iron for each patient , and significant cost savings can result from such a strategy . - pubmed
Whether ferritin should be supplanted by another parameter and whether iron itself poses an increased risk to those patients it has so beneficially served are issues that must be resolved . - pubmed
Here , we show that cross - linking of the beta ( 2 ) ( CD18 ) or beta(3 ) ( CD61 ) , but not beta ( 1 ) ( CD 29 ) integrins in the presence of a low dose of formyl - Methionyl - Leucyl - Phenylalanine ( fMLP ) enables similar 8 - s periodic RALS oscillations in suspended PMN in response to stimulation with two consecutive doses of chemoattractants . - pubmed
However , the A1 receptor agonist 2 - Chloro - N6 - cyclopentyladenosine and the A3 receptor agonists N6 - Benzyl - NECA and 1 - deoxy-1 -[ 6 -[[( 3 - iodophenyl ) methyl ] amino ]- 9H - purin-9 - yl ]- N - methyl - be ta - d - ribofuranuronamide expressed only weak inhibitory effects . - pubmed
On the other hand , NECA and CGS-21680 dose - dependently potentiated the production of IL-10 . - pubmed
The differential effect of these drugs on monocyte IL-12 and IL-10 production implies that these effects are mediated by A2a receptor signaling rather than by intracellular toxicity of ADO analogue 's metabolites . - pubmed
Moreover , CGS-21680 inhibited IL-12 production independently of endogenous IL-10 induction , because anti - IL-10 Abs failed to prevent its effect . - pubmed
The selective A2a antagonist 8 -( 3 - Chlorostyryl ) caffeine prevented the inhibitory effect of CGS-21680 on IL-12 production . - pubmed
The phosphodiesterase inhibitor Ro 20 - 1724 dose - dependently potentiated the inhibitory effect of CGS-21680 and , furthermore , Rp - cAMPS , a protein kinase A inhibitor , reversed the inhibitory effect of CGS-21680 , implicating a cAMP / protein kinase A pathway in its action . - pubmed
Activated neutrophils release a lot of mediators including toxic oxygen metabolites , elastase and cytokines which damage brain tissue . - pubmed
Therefore , we investigated roles of neutrophil elastase in the development of cerebral damage using an elastase inhibitor , ONO-5046 . - pubmed
NR1 subunit pore mutations that block Ca ( 2 + ) influx , and deletion of the NR1 cytoplasmic C - terminal domain , which functions in Ca ( 2 + ) regulation of receptor currents , decreased NMDAR mediated cell killing . - pubmed
These results implicate positive actions of the selectivity filter and of the NR1 C - terminal domain in a Ca ( 2 +)-dependent mechanism for NMDAR excitotoxicity . - pubmed
The abnormality appears to inhibit the binding of vitamin B6 to the enzyme glutamic acid decarboxylase-1 , which is needed for the biosynthesis of gamma - aminobutyric acid ( GABA ) . - pubmed
Research has shown that the emetic response is linked to serotonin and the stimulation of the 5 - HT3 receptors located in several areas of the body . - pubmed
METHODS : cDNA - expressed CYP isoforms were used to identify the isoforms that are able to mediate the N - dealkylation of perphenazine , which is considered a major metabolic pathway for the drug . - pubmed
RESULTS : CYP isoforms 1A2 , 3A4 , 2C8 , 2C9 , 2C18 , 2C19 and 2D6 were able to mediate the N - dealkylation of perphenazine . - pubmed
Ketoconazole inhibition of N - dealkylation mediated by a mixed HLM indicated that CYP3A4 accounted for about 40 % of perphenazine N - dealkylation at therapeutically relevant concentrations . - pubmed
The contribution of the CYP isoforms 1A2 , 2C19 and 2D6 amounted to 20 - 25 % each as measured by the percentage inhibition obtained by addition of furafylline , fluvoxamine or quinidine , respectively . - pubmed
HLM - mediated N - dealkylation of perphenazine accounted for 57 % of the total amount of substrate consumed during incubation . - pubmed
CONCLUSIONS : The present in vitro study suggests that CYP isoforms 1A2 , 3A4 , 2C19 and 2CD6 are primarily involved in the N - dealkylation of perphenazine . - pubmed
When R - HA was administered concomitantly with Thiop infusion , R - HA failed to attenuate the RNS - induced antidiuretic action and increases in NEOR . - pubmed
However , in the presence of pyrilamine ( a selective H1 - receptor antagonist ) or cimetidine ( a selective H2 - receptor antagonist ) infusion , R - HA attenuated the RNS - induced actions , similarly to the case without these antagonists . - pubmed
With the use of PKC alpha -- specific inhibitors ( safingol , Go6976 ) as well as the PKC delta -- specific inhibitor rottlerin , we showed that only PKC alpha plays a major role in the regulation of tumor cell migration . - pubmed
After stimulation with phorbol ester we observed a translocation and a colocalization of the activated PKC alpha at the plasma membrane to the surrounding extracellular matrix . - pubmed
The AtPLDdelta protein , of 868 amino acids , has a putative catalytic domain and a C2 domain that is involved in Ca2 +/phospholipid binding . - pubmed
The accumulation of PtdOH in response to dehydration was significantly suppressed in AtPLDdelta antisense transgenic plants . - pubmed
These results suggest that AtPLDdelta may be involved in PtdOH accumulation in the dehydration stress response . - pubmed
The lines secreted TPA , IL-6 , IL-8 and few or no thyroid - related hormones in the culture supernatant . - pubmed
METHODS : The changes of calcium channel dynamics in BSMCs of 4 asthma guinea - pig models ( 22 cells ) and 5 normal guinea - pigs ( 25 cells ) , and the inner peak calcium current in BSMCs of 5 asthma guinea - pig models ( 25 cells ) and 6 normal guinea - pigs ( 24 cells ) were determined by patch - clamp techniques . - pubmed
RESULTS : ( 1 ) The open time of calcium channel in BSMCs of asthma group was ( 1.70 + /- 0.40 ) ms , 2.32 times as much as that of control group ( 0.70 + /- 0.10 ) ms ; the close time of calcium channel in BSMCs of asthma group ( 5.7 + /- 0.6 ) ms , was significantly shorter that that of control group ( 7.9 + /- 0.4 ) ms ; The open probability in asthma group ( 0.40 + /- 0.10)% was significantly increased than that of control group ( 0.20 + /- 0.20)% , ( P & lt ; 0.01 ) ; ( 2 ) The inner peak calcium current in BSMCs of asthma group was ( -54 + /- 23 ) PA , 1.17 times as muth as that of control group ( -25 + /- 8) PA , ( P & lt ; 0.01 ) . - pubmed
It was found that nicotine self - administration profoundly decreased , in a dose - dependent manner , the expression of PSA - NCAM in the DG ; a significant effect was observed at all the doses tested ( 0.02 , 0.04 , and 0.08 mg / kg per infusion ) . - pubmed
Detailed aggregation studies revealed a dose - dependent inhibition of platelet aggregation with ristocetin by DXS 5000 , whereas aggregation with ADP , collagen , and arachidonate was unaffected . - pubmed
A direct impairment of erythrocyte membrane MRP by hypochlorous acid was shown by electrophoresis and immunoblotting ( c(50%)=478+/-36 microM HOCl ) . - pubmed
The stoichiometry of the MRP / HOCl reaction was 1:1 . - pubmed
These results demonstrate that MRP can be one of the cellular targets for the inflammatory mediator hypochlorous acid . - pubmed
The results show that glutaraldehyde combinative solution ( LacZ ) produced the most consistent and reliable results . - pubmed
Liposem significantly raised high - density lipoprotein ( HDL ) cholesterol and the HDL / low density lipoprotein + very low - density lipoprotein ( VLDL+LDL ) ratio . - pubmed
Finally , early maternal separation failed to alter expression of messenger ribonucleic acids coding for 5 - hydroxytryptamine1A or alpha1B receptors in the dorsal raphe nucleus as measured by in situ hybridization histochemistry , suggesting that functional changes in receptor sensitivity observed are not due to changes in receptor gene transcription . - pubmed
Cardiac allograft rejection is a focal inflammation and soluble markers are released into coronary sinus ( CS ) . - pubmed
In conclusion , NA and 5 - HT exerted a similar action on MN neurons ; in contrast , the effects of 5 - HT on IN and LN were more differentiated than those exerted by NA . - pubmed
Aging is associated with a rise in intracellular reactive oxygen species ( ROS ) and a loss of telomerase reverse transcriptase activity . - pubmed
Incubation with H2O2 induced the nuclear export of telomerase reverse transcriptase ( TERT ) into the cytosol in a Src - family kinase - dependent manner . - pubmed
Therefore , we investigated the hypothesis that age - related increase in reactive oxygen species ( ROS ) may induce the nuclear export of TERT and contribute to endothelial cell senescence . - pubmed
Moreover , the induction of premature senescence by low concentrations of H2O2 was completely blocked with the Src - family kinase inhibitor PP2 , suggesting a crucial role for Src - family kinases in the induction of endothelial cell aging . - pubmed
Likewise , nuclear export of TERT protein , loss in the overall TERT activity , and the onset of replicative senescence were delayed by incubation with N - acetylcysteine . - pubmed
The tests were performed at rest and while exhaling hard through a mouthpiece connected to a mercury manometer . - pubmed
Peak cortisol responses to all bCRH treatments were greater ( P & lt ; 0.05 ) than those to pretreatment concentrations . - pubmed
The ratio of mean cortisol to mean ACTH for all bCRH doses tested differed ( P & lt ; 0.05 ) from control values , indicating reactivity of the adrenals . - pubmed
Pharmacokinetic modeling of species - dependent enhanced bioavailability of trifluorothymidine by thymidine phosphorylase inhibitor . - pubmed
pyruvate kinase , alpha - enolase , triosephosphate isomerase ) , glucose oxidation ( e.g. - pubmed
Control of rHuEPO biological activity : the role of carbohydrate . - pubmed
Glycosylation analogs with altered N - linked carbohydrate content were compared with rHuEPO to elucidate the relationship between carbohydrate content and activity . - pubmed
Assays were performed to compare in vitro receptor binding and in vivo activity of rHuEPO , darbepoetin alfa , and analogs . - pubmed
CONCLUSION : Carbohydrate increases persistence of EPO , resulting in a prolonged and increased biological response in vivo , and overcoming reduced receptor - binding activity . - pubmed
[ Expression , purification , and characterization of single - chain disulfide - bond Fv ( ScdsFv ) antibody fused with targeted superantigen SEA ( D227A ) ] . - pubmed
coli BL21plusS by IPTG and purified by Q Sepharose HP column and Hiprep 26/60 Sephacryl S-200 HR column . - pubmed
Administration of MK886 , a 5LO - activity inhibitor , or MK571 , a selective cysteinyl - LT ( 1 ) receptor antagonist , largely prevented ventilator - induced lung injury . - pubmed
The AP maquette variants , AP2 and AP3 , removed heme - ligating histidines from the HP domain and included heme - ligating histidines in LP domains by selecting the b(H ) heme binding sequence from the membrane - spanning d - helix of respiratory cytochrome bc ( 1 ) . - pubmed
These represent the first examples of AP maquettes with heme and bacteriochlorophyll binding sites located within the LP domains . - pubmed
The absence of any other phycoerythrin gene in the fully sequenced genome of this organism , the previous inability to detect a gene product , and the mutation of two out of four cysteine residues , normally involved in binding chromophores , suggested that MED4 - cpeB might not code for a functional protein . - pubmed
Microsomal glutathione peroxidase ( GPx ) activity was determined as the consuming rate of NADPH . - pubmed
Athletes can return to their normal activity once a successful management strategy has addressed all components of the female athlete triad while providing adequate rest . - pubmed
Assessment of solvent residues accessibility using three Sulfo - NHS - biotin reagents in parallel : application to footprint changes of a methyltransferase upon binding its substrate . - pubmed
We present a strategy based on usage in parallel of three commercially available reagents ( Sulfo - NHS - biotin , Sulfo - NHS - LC - biotin , and Sulfo - NHS - LC - LC - biotin ) to efficiently assess the solvent accessibility of amino acids using MALDI - TOF mass spectrometry . - pubmed
Fusing octreotide tomographic construction with CT images allowed accurate determination of the area of increased uptake to be in the right transverse abdominal muscle . - pubmed
With respect to heme proteins , the corresponding visible ECD spectra , which probe the chirality of the heme environment , have been used to explore functionally relevant structural changes in the heme vicinity . - pubmed
While the physical basis of the obtained ECD signal has been analyzed by Woody and co - workers in terms of multiple electronic coupling mechanism between the electronic transitions of the heme chromophore and of the protein ( Hsu , M.C. - pubmed
Inhibition of heme oxygenase ( HO ) , the rate - limiting enzyme in heme catabolism , may be an ideal strategy for preventing neonatal jaundice . - pubmed
Although natural and synthetic heme analogs , called metalloporphyrins ( Mps ) , have been extensively investigated for this purpose , some Mps are phototoxic , affect the activity of other enzymes , or induce HO-1 transcription - properties that may limit their clinical use . - pubmed
Another class of compounds , imidazole - dioxolanes , has been shown to selectively inhibit the inducible isozyme HO-1 . - pubmed
significantly inhibited HO activity and reduced in vivo bilirubin production . - pubmed
We conclude that AZA effectively inhibits in vivo HO activity only at a high dose and that it also induces spleen HO-1 gene transcription . - pubmed
Acquiring nutrients from the environment is essential for all microbes , and the ATP - binding cassette ( ABC ) transporters are one of the major routes by which bacteria achieve it . - pubmed
, but their persistence in fully determining the properties of this system led to the experimental demonstration that QAC uptake utilizes three different substrate - binding proteins ( SBPs ) , two of which are encoded at remote locations on the genome as ' orphan ' SBPs that are each able to function with a single core ABC transporter . - pubmed
Setting time ( ST ) and compressive strength ( CS ) were measured after 1 day , 1 week and 4 weeks in phosphate buffered saline ( PBS ) solution , and the corresponding microstructure was evaluated using electron microscopy and X - ray diffraction . - pubmed
Protein C is a vitamin K - dependent anticoagulant serine protease zymogen in plasma which upon activation by the thrombin - thrombomodulin complex down - regulates the coagulation cascade by degrading cofactors Va and VIIIa by limited proteolysis . - pubmed
Recent structural and mutagenesis data have indicated that basic residues of three exposed surface loops known as 39 - loop ( Lys-37 , Lys-38 , and Lys-39 ) , 60 - loop ( Lys-62 , Lys- 63 , and Arg-67 ) , and 70 - 80 - loop ( Arg-74 , Arg-75 , and Lys-78 ) ( chymotrypsin numbering ) constitute an anion binding exosite in APC that interacts with the procoagulant cofactors Va and VIIIa in the anticoagulant pathway . - pubmed
To gain more insight in the role of galactosylation of lysines in the Gly - X - Lys repeats of collagenous proteins , we investigated the subcellular localization of GLT25D1 . - pubmed
Additionally we examined co - localization of GLT25D1 with MBL and lysyl hydroxylase 3 ( LH3 , PLOD3 ) , which is a protein able to catalyze hydroxylation of lysine residues before they can be glycosylated . - pubmed
Comparable rescue results were obtained after elevating endogenous BDNF protein levels in hippocampus with 4 daily injections of a short half - life ampakine ( positive modulator of alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionate [ AMPA ]- type glutamate receptors ) . - pubmed
The A subunits of Stx1 ( Stx1A ) and Stx2 ( Stx2A ) are ribosome inactivating proteins ( RIPs ) that inhibit translation by removing an adenine from the highly conserved alpha - sarcin ricin loop ( SRL ) of the large rRNA . - pubmed
However , Asn75 and Tyr77 were more critical for the depurination activity of Stx2A , while Arg176 was more critical for the depurination activity of Stx1A. - pubmed
Analysis of solvent accessible surface areas indicated that Asn75 and Tyr77 are more exposed in Stx2A , while Arg176 is more exposed in Stx1A , indicating that residues with higher surface exposure were more critical for enzymatic activity . - pubmed
Mental disorders were assessed using the Alcohol Use Disorder and Associated Disabilities Interview Schedule - IV version . - pubmed
At present , the described mannosidases specific for alpha1,6 - mannose linkages are very limited in number . - pubmed
Analysis of SpGH125 and CpGH125 reveal them to have exo - alpha1,6 - mannosidase activity consistent with specificity for N - linked glycans having their alpha1,3 - mannose branches removed . - pubmed
An EPR study has been carried out to investigate the structure and behaviour of the free radical formed gamma - irradiated l - alaninamide hydrochloride , dl - glutamic acid monohydrate and N -( 2 - carboxyethyl ) iminodiacetic acid powders at room temperature . - pubmed
The main target is thereby the early proximal tubule proximal tubule where secondary active transport secondary active transport of the sugar is mediated by the sodium - D : - glucose D - glucose cotransporter SGLT2 SGLT2 . - pubmed
The properties of these binding sites differ between SGLT2 and SGLT1 SGLT1 , which mediates sugar absorption sugar absorption in the intestine intestine . - pubmed
Various O- , C- , N- and S - glucosides have been synthesized with high affinity affinity and high specificity specificity for SGLT2 SGLT2 . - pubmed
Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located at the sarcin / ricin loop of the larger rRNA . - pubmed
Although several mediators including reactive oxygen species , platelet derived growth factor , transforming growth factor - beta ( TGF - beta ) and tumour necrosis factor - alpha ( TNF - alpha ) were implicated in HSC activation , the cellular signalling pathways that regulate this process remain incompletely defined . - pubmed
The TGF - beta - induced alpha - SMA expression was blocked by AACOCF ( 3 ) . - pubmed
In the current investigation , immunoblot analysis intriguingly demonstrated that Pfnek3 , a plasmodial mitogen - activated protein kinase kinase ( MAPKK ) , displayed both serine / threonine and tyrosine kinase activities in autophosphorylation reactions as well as in phosphorylation of the exogenous myelin basic protein substrate . - pubmed
Further probing into the regulatory mechanism of Pfnek3 also revealed tyrosine phosphorylation to be a crucial factor that stimulates its kinase activity . - pubmed
Through biocomputational analyses and functional assays , tyrosine residues Y117 , Y122 , Y172 , and Y238 were proposed as phosphorylation sites essential for mediating the catalytic activities of Pfnek3 . - pubmed
In addition to the expected HR adaptation , the VR response to beta - adrenoceptor stimulation with Iso and to muscarinic receptor blockade with atropine thus included alterations previously observed in congenital and acquired long QT syndromes , demonstrating substantial overlap between physiological and pathophysiological electrophysiology . - pubmed
Importantly , N - acetyl cysteine ( NAC ) treatment effectively blocked apoptosis via the caspase-9 and caspase-3 pathways while NAC attenuated the inhibition of mitochondrial complex I activity as well as the oxidative metabolism of dopamine ( DA ) . - pubmed
In the response of cellular antioxidant systems to CPF , we found that CPF treatment increased HO-1 expression while the expression of CuZnSOD and MnSOD was reduced . - pubmed
In addition , we found that CPF treatment activated MAPK pathways , including ERK 1 / 2 , the JNK , and the p38 MAP kinase in a time - dependent manner . - pubmed
NAC treatment abolished MAPK phosphorylation caused by CPF , indicating that ROS are upstream signals of MAPK . - pubmed
Interestingly , MAPK inhibitors abolished cytotoxicity and reduced ROS generation by CPF treatment . - pubmed
Our results demonstrate that CPF induced neuronal cell death in part through MAPK activation via ROS generation , suggesting its potential to generate oxidative stress via mitochondrial damage and its involvement in oxidative stress - related neurodegenerative disease . - pubmed
The aim of this study was to compare the concordance of immunophenotype established with routine cytopathology ( slides stained with Giemsa solution ) and immunocytochemistry according to CD3 and CD79 alpha immunoreactivity . - pubmed
Cytosolic phospholipase A2 alpha / arachidonic acid signaling mediates depolarization - induced suppression of excitation in the cerebellum . - pubmed
Intracellular Ca ( 2 + ) elevation is critical for the endocannabinoid production and DSE . - pubmed
Nevertheless , how elevated Ca ( 2 + ) leads to DSE is unclear . - pubmed
The degradation enzyme of 2 - arachidonoylglycerol ( 2 - AG ) , monoacylglycerol lipase ( MAGL ) , blocked DSE , while another catabolism enzyme for N - arachidonoylethanolamine ( AEA ) , fatty acid amide hydrolase ( FAAH ) , did not affect DSE . - pubmed
Co - application of paxilline reversed the blockade of DSE by internal K(+ ) , indicating that large conductance Ca ( 2 +)- activated potassium channel ( BK ) is sufficient to inhibit cPLA(2)alpha / arachidonic acid - mediated DSE . - pubmed
In addition , we showed that the release of 2 - AG was independent of soluble NSF attachment protein receptor ( SNARE ) , protein kinase C and protein kinase A. - pubmed
, rivaroxaban , apixaban and edoxaban ) and thrombin inhibitors ( e.g. - pubmed
Despite coils are mostly used embolic material in pseudoaneurysm , embolization with histoacrylate glue can be highly effective and quick treatment modality for post - traumatic hepatic artery pseudoaneurysm , especially in cases when it is impossible to use standard materials . - pubmed
Predicting the highest intensity ion in multiple charging envelopes observed for denatured proteins during electrospray ionization mass spectrometry by inspection of the amino acid sequence . - pubmed
A simple , manual method for predicting the highest intensity charge states ( HICS ) of denatured protein ions generated by electrospray ionization based on inspection of the proteins ' amino acid sequence is proposed . - pubmed
Upon reduction of the disulfide bonds of these proteins , the experimental HICS became closer to the predicted values , suggesting that charging lower than the prediction can be attributed to conformational inflexibility of those proteins . - pubmed
This paper reviews the discovery and history of the use of irreversible monoamine oxidase ( MAO ) inhibitors ( MAOIs ) such as phenelzine , tranylcypromine and isocarboxazid , as well as the second generation selective and reversible MAOIs such as the MAO - A inhibitor , moclobemide and the MAO - B inhibitor , selegiline . - pubmed
CM - HP - beta - CD showed the strongest insulin - binding ability and the best transport properties compared with the other beta - cyclodextrin derivatives we evaluated . - pubmed
RESULTS : After PCR correction , ACPR at D28 was 100 % for A - L , 96.55 % for AQ - SP , and 100 % for AQ - AS , with no significant difference between the three combinations ( p = 0.36 ) . - pubmed
Some NR activity ( 15 % ) could be restored by adding unphysiologically high concentrations of molybdate to the culture medium in two of the cnx - lines ( lines 3 and 4 ) . - pubmed
Could pro - BNP , uric acid , bilirubin , albumin and transferrin be used in making the distinction between stroke subtypes ? - pubmed
We observed significantly higher values within 24 hours of uric acid ( p & lt ; 0.05 ) , significantly lower values within 24 hours of transferrin ( p & lt ; 0.05 ) , significantly lower values at 7 days of albumin and transferrin ( p & lt ; 0.001 ) , significantly higher values at 7 days of uric acid and bilirubin ( p & lt ; 0.001 ) . - pubmed
( 18 ) FDG - PET and ( 18 ) FDG - PET / CT might be useful to confirm suspected pancreatic cancer . - pubmed
Pooled estimates for ( 18 ) FDG - PET / CT were : sensitivity 90 % , specificity 76 % , PPV 89 % , NPV 78 % and accuracy 86 % . - pubmed
CONCLUSION : Both ( 18 ) FDG - PET and ( 18 ) FDG - PET / CT offer no benefit over the current primary diagnostic tools in diagnosing pancreatic cancer . - pubmed
However , the ( 18 ) FDG - PET / CT systems are still improving . - pubmed
Yet , ( 18 ) FDG - PET / CT may have a role in the staging of pancreatic cancer , in survival prediction , and may add to other diagnostic information , like histology . - pubmed
Evaluation of two commercial omalizumab / free IgE immunoassays : implications of use during therapy . - pubmed
The oxidative breakdown of carotenoid evidences the formation of apocarotenoids through carotenoid cleavage dioxygenases ( CCDs ) . - pubmed
Comprehensive identification of disulfide bonds using non - specific proteinase K digestion and CID - cleavable crosslinking analysis methodology for Orbitrap LC / ESI - MS / MS data . - pubmed
Here , we describe an updated application of our CID - cleavable crosslink analysis software and our crosslinking analysis with non - specific digestion methodology for the robust and comprehensive determination of disulfide bonds in proteins , using Orbitrap LC / ESI - MS / MS data . - pubmed
Anthropometric measurements , as well as dietary intake , selected vital signs , resting energy expenditure , blood lipids , glucose , insulin , and selected adipose - derived hormones were measured at baseline , and weeks 2 , 12 , and 24 . - pubmed
Both groups experienced significant decreases in body mass index , fat - free soft tissue mass , body fat percentage , waist and hip circumferences and serum triglycerides , total cholesterol , and leptin ( all P & lt ; 0.001 ) . - pubmed
PURPOSE : To investigate the association between the single nucleotide polymorphisms ( SNPs ) of the adiponectin gene and nonalcoholic fatty liver disease ( NAFLD ) as well as the impact of the interaction of multiple SNPs on NAFLD risk , based on a Chinese population study . - pubmed
Exendin-4 protects HUVECs from t - BHP - induced apoptosis via PI3K / Akt - Bcl-2 - caspase-3 signaling . - pubmed
METHODS : HUVECs were treated with 100 micromol / L t - BHP for 4 h , following pretreatment with 2.5 - 25 nmol / L exendin-4 . - pubmed
The percentage of apoptotic cells was determined by fluorescence microscopy after Hoechst / PI staining . - pubmed
Exendin-4 reversed t - BHP - mediated inhibition of Akt phosphorylation , which was abrogated by the PI3 K inhibitor , wortmannin . - pubmed
Sirtuins ( SIRT1 - 7 ) are a family of nicotine adenine dinucleotide ( NAD+)-dependent enzymes that catalyze post - translational modifications of proteins . - pubmed
Ovalbumin with Glycated Carboxyl Groups Shows Membrane - Damaging Activity . - pubmed
Our results show a significant role for RNA transcription : 50 % of upregulated transcription factors were related to hypoxia responses , differentiated modules were significantly enriched for oxygen transport , and importantly , divergent EPAS1 functional variants with a refined co - expression network were identified . - pubmed
Aminopeptidases are exopeptidases that catalyze the hydrolysis of amino acid residues from the N terminus of peptides and proteins . - pubmed
The complex formation of the ion - pair formed between the anionic chelate of Ge ( IV )- 4 - nitrocatechol ( 4 - NC ) and the cation of 1,4 - diphenyl-3 -( phenylamino )- 1H-1,2,4 - triazole ( Nitron , Nt ) in the liquid - liquid extraction system Ge ( IV )- 4 - NC - Nt - H2O - CHCl3 was studied by spectrophotometry . - pubmed
Herein is reported the design , fabrication , and test of flexible , thin film silicon photodetectors ( PDs ) bonded to a flexible substrate designed for use in conformal DRS . - pubmed
Furthermore , antibiotic resistance phenotypes , including macrolide and vancomycin resistance , as well as virulence factor - encoding genes [ asa1 , esp - fm , esp - fs , hyl , and cyl ( B ) ] were investigated in all isolates . - pubmed
METHODS AND RESULTS : The association between gestational age ( in weeks ) obtained from Child Health Services records and CRF , estimated from field and laboratory tests and expressed by maximal oxygen uptake level through adolescence to young adulthood , was examined in 791 participants in the Northern Ireland Young Hearts Study , all singletons born at term . - pubmed
In this study , we demonstrated that sustained stimulation of beta - ARs significantly induced synthesis and secretion of growth factors , including connective tissue growth factor ( CTGF ) and vascular endothelial growth factor ( VEGF ) , via the cAMP - dependent and protein kinase A ( PKA)-dependent pathways . - pubmed
In addition , isoproterenol ( ISO)-mediated synthesis and secretion of CTGF and VEGF through the beta1 - AR and beta2 - AR subtypes . - pubmed
The probability based LiDAR samples capture variations of structure and carbon across edaphic and climate conditions , and provide an alternative approach to national ground inventory for efficient and precise assessment of forest carbon resources for emission reduction ( ER ) programs . - pubmed
The UBLRPA products were identified visually using the PLFD through dual hybridization ( a PNA probe on the gold nanoparticle ( Au - NP ) was combined with a universal linker on the end of the products ; a PNA capture probe was used on the test line and a universal linker on the other end of the products ) . - pubmed
The targets of highly connected miRNA ( = 12 ) indicated the significant biological pathways and gene ontology ( GO ) terms , such as MAPK and Wnt signaling and magnesium - ion binding , which are associated with apoptotic activities . - pubmed
We performed histological examination ( with hematoxylin and eosin staining ) , immunohistochemical examination ( with use monoclonal antibodies CD90 ( 1:25 ; Abcam , UK ) , SD105 ( 1:500 ; Abcam , UK ) , CD44 ( 1:25 ; Dako ) , SD73 ( 1:200 , Abcam , UK ) , and electron microscopy ( by microscope Philips / FEI Corporation , Eindhoven , Holland ) . - pubmed
Nongenomic actions of thyroid hormone are initiated by the hormone at receptors in the plasma membrane , in cytoplasm , or in mitochondria and do not require the interaction of nuclear thyroid hormone receptors ( TRs ) with their primary ligand , 3,5,3 '- triiodo - l - thyronine ( T3 ) . - pubmed
Reviewed here are nongenomic effects of the hormone - T3 or , in some cases , l - thyroxine ( T4)-that are initiated at ( a ) truncated TRalpha isoforms , e.g. - pubmed
Loaded KOH and/or Ba ( OH ) 2 were identified by SEM - EDX and BET analysis , respectively , and the CO2 adsorption performances were confirmed using a gas flow system with GC . - pubmed
The T7 RNA polymerase / T7 promoter overexpression system , which is an isopropyl - beta - d - thiogalactopyranoside ( IPTG)-inducible gene overexpression system ( T7 - dependent inducible overexpression system ) , was modified to be a constitutive overexpression system . - pubmed
The F1.5 scores of the first 12 key indicators peaked at iteration ( 0.893 ) , including hemoglobin ( Hb ) , calcium ( CA ) , red blood cell count ( RBC ) , mean platelet volume ( MPV ) , mean erythrocyte hemoglobin concentration ( MCH ) , systolic blood pressure ( SBP ) , platelet count ( PLT ) , magnesium ( MG ) , lymphocyte ( LYM ) , glucose ( GLU , blood gas analysis ) , glucose ( GLU , blood biochemistry ) and diastolic blood pressure ( DBP ) . - pubmed
This effect was reversed by the cannabinoid 1 ( CB1 ) receptor antagonist , AM251 ( 1 mg / kg , ip ) , but not the PPARalpha antagonist , MK886 ( 1 mg / kg , ip ) . - pubmed
MWD did not modify levels of the endocannabinoids 2 - AG and AEA , nor those of the PPARalpha ligands , OEA and PEA , in any region evaluated . - pubmed
Spiperone elicited inverse agonist inhibitory sigmoid isotherms in all membrane preparations while WAY100635 was mostly ' silent ' for Galphai3 activation . - pubmed
Divergent roles of Kupffer cell TLR2 / 3 signaling in alcoholic liver disease and the protective role of EGCG . - pubmed
We also identified a novel ALD - protective agent EGCG which directly interacted with Kupffer cell TLR2 / 3 to induce IL-10 production . - pubmed
Deficiency of IL-10 aggravated ALD injury and blunted EGCG - mediated hepatoprotection while depletion of Kupffer cells partially recovered liver injury but abolished EGCG 's actions . - pubmed
A 3 months 40 mg pantoprazole therapy was ineffective in reduction of VP complications in our study group . - pubmed
Treatment with a low - dose combination of one antiandrogen and antimineralocorticoid drug ( spironolactone ) and two insulin sensitizers ( pioglitazone / metformin ) ( SPIOMET ) is particularly effective in improving these metabolic derangements . - pubmed
This is consistent with the effects of pioglitazone and spironolactone inducing CXCL14 expression and promoting a brown - like phenotype in adipocytes . - pubmed
Infarct size was not significantly different between the two groups ; however , the DFP group demonstrated earlier resolution of hemorrhage ( by T2 * imaging ) and edema ( by T2 imaging ) . - pubmed
Squalene and cholesterol in the balance at the ER membrane . - pubmed
The treatments were as follows : 0 , 25 , 50 , and 75 mg / kg / day of cadmium chloride ( CdCl2 ) by oral gavage from PND 21 to observation of first vaginal opening ( VO ) . - pubmed
Holstein heifers ( n = 51 ; 10.5 to 14.5 mo , and 325.0 + - 21.1 kg of body weight ) were superovulated ( n = 69 events ) for the collection of embryos using a protocol based on sequential administration of FSH for follicle superstimulation and GnRH to induce ovulation . - pubmed
A synthesized thio - anthraquinone analogue ( 3 ) was purified by column chromatography , and its chemical structure was characterized by spectroscopic methods such as Fourier - transform infrared spectroscopy ( FT - IR ) , nuclear magnetic resonance ( NMR ) spectroscopy , mass spectrometry ( MS ) , and ultraviolet ( UV)-visible spectrophotometry . - pubmed
However , after the reaction with H2O2 and the 30 mM trisodium citrate dihydrate mixture in the presence of an iron sulfate ( II ) solution , a single peak was observed , producing a stable and reddish / brownish homogenous solution ( lambda max = 304 nm ) . - pubmed
METHODS : Part 1 : An in vitro catalytic reaction comprising the ppGalNAc T2 enzyme , uridine-5 '- diphospho - N - galactosamine ( UDP - GalNAc ) and an 11 - amino acid peptide substrate , was used to assess the enzyme activity of the ppGalNAc T2 enzyme in macromolecular crowding environment respectively with bovine serum albumin ( BSA ) , polyethylene glycol ( PEG2000 ) , Dextran70 and Ficoll70 at different concentration and temperature . - pubmed
Macromolecular crowding was simulated using PEG2000 at the concentrations of 50 , 100 , and 200 g / L. Deglycosylated - MUC5AC ( d - MUC5AC ) and glycosylated MUC5AC ( g - MUC5AC ) were denatured by GdnHCl and renatured by dilution in a refolding buffer . - pubmed
RESULTS : Enzyme activityof ppGalNAc T2 was observed to increase with increasing crowding agent concentration , with highest enzyme activity at 200 g / L. Compared with the group in the absence of crowding reagent , the refolding yield of g - MUC5AC and d - MUC5AC were reduced significantly in the presence of different concentrations of PEG2000 ( 200 , 100 , and 50 g / L ) . - pubmed
Compared with the dilute solution , aggregation increased significantly in the presence of PEG2000 , especially at 200 g / L. Moreover , in the crowded reagent with the same concentration , the refolding yield of d - MUC5AC was higher than that of g - MUC5AC , whereas the degree of aggregation of d - MUC5AC was lower than that of g - MUC5AC . - pubmed
Chitinases play an important role in many industrial processes , including the preparation of oligosaccharides with potential applications . - pubmed
The hydrolytic products derived from colloidal chitins comprised mainly ( GlcNAc ) 2 and GlcNAc , indicating that rChi1602 is a GH18 processive chitinase in conformity with its hypothetical structure . - pubmed
However , rChi1602 showed traces of beta - N - acetylglucosaminidase activity on substrates such as powder chitin , chitosan , and ethylene glycol chitin . - pubmed
Myelin Oligodendrocyte Glycoprotein Antibody Disease ( MOGAD ) represents a demyelinating disorder for which tocilizumab , an anti - IL6 receptor , has been tested to prevent disabling relapses . - pubmed
The enzyme can utilize the A - particle endogenous RNA as template as shown by analysis of the early and late DNA products of the endogenous reaction by CsSO4 isopycnic gradient centrifuation and hybridization of purified 70S or 35S A - particle RNA with the purified complementary DNA product . - pubmed
Only one infant developed HMD out of 170 with amniotic fluid PAPase specific activity equal to or greater than 50 nmoles of orthophosphate released X mg.-1 of protein X hr. - pubmed
A similar binding pattern is produced in control membranes by treatment with Triton X-100 , phospholipase C , or glycerophosphoethanolamine . - pubmed
Substrate activity of ( adenosine triphosphato ) tetraamminecobalt ( III ) with yeast hexokinase and separation of diastereomers using the enzyme . - pubmed
When an efficient SOD mimic operating in vitro is introduced into cells , the following requirements are needed in order that this compound will catalyze O2- dismutation efficiently : it should be non toxic , stable , has a long metabolic half life , does not form ternary complexes with the cell components , its reduced form reacts slowly with molecular oxygen , should be able to cross cell membranes and also to reach lipophilic or hydrophobic regions . - pubmed
The mean proportion of bilirubin removed by routine plasma exchange was 29 % with a mean of plasma exchange volume of 2.1 L. In the plasma perfusion with Bespore and BR , the removal rates were 37 % and 42 % , respectively . - pubmed
From the results of tunicamycin treatment and N - glycosidase F digestion , it was demonstrated that Namalwa - derived hGM - CSF was highly glycosylated at two potential N - glycosylation sites and several O - glycosylation sites as previously shown for naturally occurring hGM - CSF . - pubmed
To investigate the role of carbohydrate moieties of hGM - CSF , we isolated each form of hGM - CSF and examined its biological properties . - pubmed
From these findings , we concluded that N - linked carbohydrate moieties of hGM - CSF play conflicting physiological roles in the efficacy of the protein in vivo but that O - linked carbohydrate moieties do not have such effects . - pubmed
Optimization of techniques in screening CT of the sinuses . - pubmed
The number of screening examinations of the sinuses performed with CT has markedly increased owing to the widespread and increasing use of endoscopic sinonasal surgery . - pubmed
Three aspects of direct coronal imaging of the paranasal sinuses were investigated : ( 1 ) preparation of the patient prior to the examination ; ( 2 ) technical factors of the CT study , including positioning of the patient , optimal coronal angle , slice thickness , and CT exposure factors ; and ( 3 ) data display . - pubmed
Forskolin is often used to activate adenylate cyclase in studies relating adenosine 3 ' , 5 '- cyclic monophosphate ( cAMP ) to the modulation of membrane current . - pubmed
Furthermore , both IA and IK.V were reduced by 1,9 - dideoxy - forskolin , a derivative of forskolin that does not activate adenylate cyclase . - pubmed
Bath application of a modified form of forskolin ( 7 - deacetyl-6 -[ N - acetylglycyl ]- forskolin ) , which has enhanced water solubility and activates adenylate cyclase , reduced IK.S , but did not alter either IA or IK.V. - pubmed
Thus it appears that certain derivatives of forskolin can be used to activate adenylate cyclase and avoid some of the nonspecific actions on membrane current that are associated with forskolin . - pubmed
During early follicular development , FSH binds to granulosa cells of primary follicles to stimulate production of estradiol by the induction or enhancement of aromatase synthetase ( 37 , 336 , 337 ) . - pubmed
Estradiol , in turn , induces proliferation of granulosa cells ( 338 - 344 ) and increases the sensitivity of the follicle to further gonadotropin stimulation ( 12 , 339 , 345 - 349 ) . - pubmed
Estradiol can synergize with gonadotropins to increase ovarian weight , enhance proliferation of granulosa cells , and promote growth of preantral follicles and antrum formation ( 345 , 347 , 350 - 352 ) . - pubmed
In addition , estradiol enhanced the responsiveness of granulosa cells to FSH and LH by increasing synthesis of progesterone ( 353 , 354 ) . - pubmed
The generalized enhancement of gonadotropin action by estradiol is partially mediated by FSH - induced accumulation of cAMP . - pubmed
Estradiol not only enhances gonadotropin stimulation of LH and FSH receptors in granulosa cells ( 357 , 348 ) but is required for FSH induction of FSH receptors ( 359 , 360 ) . - pubmed
Estradiol alone can increase numbers of its own receptor in granulosa cells ( 350 ) as well as increase its own production by stimulating aromatase activity ( 361 ) . - pubmed
Estradiol secreted by the dominant follicle has a positive feedback effect on the hypothalamus and pituitary , enhancing gonadotropin secretion and ensuring the preovulatory gonadotropin surges ( 362 ) . - pubmed
The increased gonadotropins can further increase the production of estradiol which , in turn , enhances its own production . - pubmed
A compartment syndrome developed the day after heparinization and was adequately treated by discontinuation of heparin , aspiration and rest . - pubmed
N - ethoxycarbonyl-2 - ethoxy-1,2 - dihydroquinoline , amiloride analogues , and renal Na +/ H + antiporter . - pubmed
N - ethoxycarbonyl-2 - ethoxy-1,2 - dihydroquinoline ( EEDQ ) is a carboxyl - activating agent and has been shown to inhibit the renal Na +/ H + antiporter . - pubmed
The purposes of the present studies were to characterize the kinetics of inhibition of the Na +/ H + antiporter by EEDQ and to determine whether amiloride analogues affect the ability of EEDQ to inhibit the rate of Na+/H+ exchange . - pubmed
Cyclosporine functions in the early sequence of T cell activation by regulating the production of messenger RNA for lymphokines synthesis . - pubmed
Cyclosporine does not inhibit the synthesis of lymphokine once the lymphokine message is present in the cell . - pubmed
Prevention of post - tonsillectomy pain with analgesic doses of ketamine . - pubmed
In serum - free medium the monoclonal antibody produced a 45 % reduction of cells in S phase by thymidine labeling index without modification of the growth fraction as determined by primer - dependent alpha - DNA polymerase labeling index . - pubmed
They have been identified as 1 - monomethylphosphoinositol 4,5 - bisphosphate and 1 - monomethylphosphoinositol 4 - phosphate by comparison to a synthesized standard using HPLC chromatography , paper electrophoresis , and enzymatic dephosphorylation with inositol polyphosphate 5 - phosphomonoesterase and intestinal alkaline phosphatase [ orthophosphoric - monoester phosphohydrolase ( alkaline optimum ) , EC 3.1.3.1 ] . - pubmed
[ Effect of actinomycin D on the stimulation of water and sodium transport with antidiuretic hormone ] . - pubmed
Rat liver phenylalanine hydroxylase , an iron enzyme . - pubmed
Dopamine and noradrenaline receptor stimulation : reversal of reserpine - induced suppression of motor activity . - pubmed
What lectins and carbohydrates are made for ? - pubmed
The nucleotide sequence of rabbit embryonic globin gene beta 3 . - pubmed
Increase of L - lysine concentration in the luminal perfusate to 20 mM did not reduce JHCO3 further nor did it influence Jv.l When 10 mM L - lysine was added to the capillary perfusate , a 13 % reduction in JHCO3 was observed ( NS ) . - pubmed
The monolayer culture was used in morphologic , cytochemical and cytoenzymatic studies , and the culture medium was used for assay of thyroglobulin and thyroxin . - pubmed
We found that addition of aminoacids with SH or S -- S groups to the culture medium causes stimulation of protein synthesis overstrain on the oxydases system at the expense of dehydrogenases . - pubmed
These included ultraviolet ( UV ) absorption , isoelectric points , sulfhydryl content , extinction coefficients , and circular dichroism ( CD ) . - pubmed
The effect of glycerol on the conformation of sigma , core , and holoenzyme was monitored by circular dichroism measurements . - pubmed
In the far - ultraviolet , the residue ellipticity at 220 nm ( [ theta]220 ) increased approximately 15 % from 0 to 10 % glycerol for both core and holoenzyme . - pubmed
In the near - ultraviolet , the residue ellipticity at a peak near 280 nm also varied with glycerol concentration , decreasing in intensity by about 50 % with holoenzyme , when glycerol was raised from 5 to 10 % , then increasing at still higher glycerol contents . - pubmed
The presence of 10 % glycerol in the assay buffer increased the activity of the enzyme . - pubmed
Development of dyes that can be exited at 488 nm and have emission maximum in the far red area has made three - colour protocols , together with fluorescein isothiocyanate ( FITC ) and R - phycoerythrin ( PE ) , possible in most clinical flow cytometers . - pubmed
Neuroendocrine and haemodynamic responses to abdominal aortic cross clamp and release during high - dose opiate - oxygen - isoflurane anaesthesia . - pubmed
FSH - induced estradiol production by granulosa cells from large follicles ( expressed as pg estradiol/10(5 ) cells/24 h ) was not affected ( p & gt ; 0.05 ) by 0.1 , 1.0 , or 10 ng / ml of interleukin ( IL )- 6 , whereas 30 and 100 ng / ml of IL-6 inhibited ( p & lt ; 0.05 ) FSH - induced estradiol production by 32 % in cells from large follicles . - pubmed
In comparison , all doses of IL-6 inhibited ( p & lt ; 0.05 ) FSH - induced estradiol production in cultures of cells from small follicles ; maximal inhibition was achieved ( 75 % decrease ) with & gt ; or = 10 ng / ml . - pubmed
Ribonucleotide reductase is expressed in actively dividing cells and generates deoxyribonucleotides for DNA synthesis . - pubmed
These sera recognized different P450IID6 peptide sequences consisting of amino acids ( aa ) 200 - 214 and aa 271 - 339 . - pubmed
Discovery of potent isoxazoline glycoprotein IIb / IIIa receptor antagonists . - pubmed
Using the isoxazoline as a common structural feature , three series of glycoprotein IIb / IIIa receptor antagonists were evaluated , culminating in the discovery of XR299 ( 30 ) . - pubmed
Nicks generated by the enzyme were resealable with T4 DNA ligase , indicating that the enzyme produces 5'-p and 3'-OH ends . - pubmed
Influence of n-3 fatty acid supplementation on the endogenous activities of plasma lipases . - pubmed
Low - density - lipoprotein - cholesterol concentrations increased by 25 % in the latter group ( P = 0.06 ) . - pubmed
An SH3 - domain - containing protein has been identified that interacts with sequences in the cABL carboxyl terminus . - pubmed
Additionally , these results provide further evidence for the importance of the cABL carboxyl terminus and its binding proteins in the regulation of cABL function . - pubmed
The gene product of the yeast VPS1 gene is a member of a family of high - molecular - weight GTP - binding proteins that are involved in diverse cellular processes . - pubmed
DNA sequence analysis of the mutant vps1 allele ( vps1d-293 ) revealed a single point mutation , resulting in an amino acid exchange at position 293 from Ala to Asp . - pubmed
Following earlier work on cystine - bridged peptides , cyclic phosphopeptides containing nonreducible mimics of cystine were synthesized that show high affinity and specificity toward the Src homology ( SH2 ) domain of the growth factor receptor - binding protein ( Grb2 ) . - pubmed
The enzyme was solubilized by detergent treatment ( 2 % [ w / v ] Triton X-100 ) and purified by heparin - Sepharose and Q - Sepharose chromatography . - pubmed
While Mg ( 2 + ) was the preferred divalent cation , Mn ( 2 + ) could partly substitute in the reaction catalyzed by the QII enzyme but not in that catalyzed by QI . - pubmed
Mn ( 2 + ) acted synergistically with suboptimal Mg ( 2 + ) concentrations to activate not only the QII enzyme , but also to some extent QI . - pubmed
Both enzymes were inhibited by millimolar concentrations of Ca ( 2 + ) and micromolar concentrations of wortmannin . - pubmed
The apparent molecular mass for QI was 120 kD , which was determined by SDS - PAGE and western blotting using an antibody against a peptide unique for lipid kinases and the binding of ( 3 ) H - wortmannin , and for QII 65 kD as determined by immunodetection and renaturation of PtdIns kinase activity in the 65 - kD region of polyacrylamide gels . - pubmed
Modulation of apoptotic and inflammatory genes by bioflavonoids and angiotensin II inhibition in ureteral obstruction . - pubmed
We investigated the effect of bioflavonoids and angiotensin II inhibition on apoptotic and inflammatory gene expression in a model of unilateral ureteral obstruction ( UUO ) . - pubmed
Treatment with curcumin increased HO-1 expression fourfold ( P & lt;0.05 ) . - pubmed
Bioflavonoids and angiotensin inhibitors are able to attenuate the expression of these genes and thus may be beneficial in renal protection . - pubmed
Trace - level determination of 1,4 - dioxane in water by isotopic dilution GC and GC - MS . - pubmed
This investigation established that 1,4 - dioxane can be determined in water by various techniques including direct aqueous injection ( DAI ) gas chromatography ( GC ) and purge and trap GC - mass spectrometry ( MS ) . - pubmed
Purge and trap GC - MS is limited by 1,4 - dioxane 's poor purge efficiency , resulting in detection limits up to 100 times greater than the efficiently purged volatile organic compounds . - pubmed
Isotopic dilution quantification was also possible using a non - selective GC detector owing to the high efficiency of capillary GC columns that resolve the deuterium - labeled solvent from the natural isotopes . - pubmed
The ligated benzonitriles in the platinum ( II ) complex [ PtCl2 ( PhCN ) 2 ] undergo metal - mediated [ 2 + 3 ] cycloaddition with nitrones - ON +( R3 )= C ( R1 )( R2 ) [ R1 / R2 / R3 = H / Ph / Me , H / p - MeC6H4 / Me , H / Ph / CH2Ph ] to give delta 4 - 1,2,4 - oxadiazoline complexes , [ PtCl2(N = C(Ph)O - N(R3)-C(R1)(R2))2 ] ( 2a , 4a , 6a ) , as a 1:1 mixture of two diastereoisomers , in 60 - 75 % yields , while [ PtCl2(MeCN)2 ] is inactive toward the addition . - pubmed
METHODS : The Fluent 4.4.7 code was run on a Silicon Graphic Inc. computer ( 4 - CPUx185 MHz ) in the CFD simulation . - pubmed
Shifting from E1 to E2 form is induced by replacing Na + by K+ ions . - pubmed
The detection threshold allowed the possibility to detect a change affecting five amino acids out of the 1324 that compose the H +/ K +- ATPase molecule . - pubmed
Evidence that conformational changes occur in an identical system was brought by monitoring the fluorescence of fluorescein isothiocyanate - labeled H +/ K +- ATPase in similar conditions . - pubmed
In addition to the expected post - transplantation and hematologic malignancy cases , other risk groups identified included those with chronic asthma and steroid use , acquired immunodeficiency syndrome , thermal burn , hepatic failure , and postoperative infection . - pubmed
The complication group showed a smaller HPD ( P = 0.022 ) , reduced pupillary responses in the cocaine ( P = 0.037 ) and pholedrine tests ( P & lt ; 0.001 ) , and anisocor pupil sizes after instillation of the eye drops ( P = 0.034 ) . - pubmed
Oxygen consumption ( VO2 ) , carbon dioxide production ( VCO2 ) , and respiratory exchange ratio ( RER ) were measured at 15 - min intervals . - pubmed
Compared to the CON diet , FAT diet consumption for 5 - 10 weeks was associated with an altered metabolic response to low - intensity exercise , as evidenced by a more than 30 % reduction in the production and utilisation of glucose ; a decrease in RER ; a decrease in the estimated rate of whole - body carbohydrate utilisation ; and an increase in the whole - body rate of lipid oxidation during exercise . - pubmed
Subsequently , we found AmpG to be a permease required for recycling of murein tripeptide and uptake of anhydro - muropeptides . - pubmed
These unique substrates for AmpG , which contain murein peptides linked to GlcNAc - anhMurNAc , are produced by turnover of the cell wall during logarithmic growth . - pubmed
AmpG permease is sensitive to carbonylcyanide m - chlorophenylhydrazone , demonstrating that AmpG permease is a single - component permease and that transport is dependent on the proton motive force . - pubmed
The leucine 7 to proline 7 ( Leu7Pro ) polymorphism in the signal peptide of prepro - NPY is associated with increased blood lipid levels , accelerated atherosclerosis , and diabetic retinopathy . - pubmed
Subjects with the Leu7Pro polymorphism had significantly lower plasma NPY and norepinephrine concentrations , lower insulin concentrations , higher glucose concentrations , and lower insulin - glucose ratio in plasma than the controls . - pubmed
Systolic and diastolic blood pressure , serum free fatty acid and plasma leptin , ACTH , cortisol , LH , FSH , TSH , free thyroxin , and melatonin concentrations were similar during the 24 - h period , compared with controls . - pubmed
Thioredoxin 2 , an oxidative stress - induced protein , contains a high affinity zinc binding site . - pubmed
Both thioredoxins are capable of reducing disulfide bonds using a conserved pair of cysteine residues present in a WCGPC motif . - pubmed
We show here that Trx2 contains zinc in a 1:1 stoichiometry , making it the first identified zinc - binding thioredoxin . - pubmed
The zinc center of Trx2 binds zinc with a very high affinity ( Ka of & gt;1018 m-1 ) . - pubmed
The zinc - free protein conserves its reductase activity . - pubmed
Previous studies have focused mainly on elucidating the cellular mechanisms responsible for membrane recycling under conditions similar to the stress - activated state and indicate a clathrin / dephosphin - mediated retrieval via coated pits . - pubmed
We show that block of the calcium - mediated protein phosphatase 2B , a common step in clathrin - mediated processes , did not alter endocytosis elicited at basal firing levels . - pubmed
After HP test results were confirmed to be positive , anti - HP therapy consisting of omeprazole , clarithromycin , and amoxicillin was administered for 2 weeks . - pubmed
2,6 - Pyridinedicarboxylic acid ( PDC ) and n - hexadecyltrimethylammonium hydroxide ( CTAOH ) were selected to compose a background electrolyte ( BGE ) , where PDC was used as chromophore and CTAOH functioned as electroosmotic flow ( EOF ) modifier to reduce / eliminate EOF . - pubmed
Beryllium - induced tumor necrosis factor - alpha production by CD4 + T cells is mediated by HLA - DP . - pubmed
B7 - 1 and B7 - 2 are closely related molecules expressed on antigen - presenting cells that provide costimulatory signals for T - cell activation . - pubmed
Halogenated aromatic hydrocarbons ( HAHs ) , such as 2,3,7,8 - tetrachlorodibenzo - p - dioxin ( TCDD ) , produce a wide variety of biological and toxic effects mainly through the aryl hydrocarbon receptor ( AhR)-dependent mechanism . - pubmed
After the binding of HAHs , the AhR subsequently transforms its form in order to interact with a specific DNA sequence , the dioxin responsive element ( DRE ) . - pubmed
In this study , we have developed a simple method for quantitative assessment of the transformation state of AhR based on an enzyme - linked immunosorbent assay ( ELISA ) combined with southwestern chemistry technique ( SW - ELISA ) that detects the complex of transformed AhR : fluorescein isothiocyanate ( FITC)-labeled DRE probe . - pubmed
Furthermore , SW - ELISA provides the confirmation that flavonoids , the potent antagonists for AhR as reported previously , show the inhibitory effects on TCDD - induced AhR transformation . - pubmed
The model is consistent with current biophysical , biochemical , and structural data ( notably , mass - per - unit - length measurements by scanning transmission electron microscopy that gave one subunit rise per 0.47 nm ) and is readily adaptable to other amyloids , for instance the core of Sup35p filaments and glutamine expansions in huntingtin . - pubmed
EPR study has shown that the anticancer agent vanadocene dichloride ( Cp2VCl2 ) interacts with carbonate contained in physiological solutions . - pubmed
Both of the expression systems produced alpha - apoprotein in a fully functional form as can judged by its ability to form a structural subunit with native beta - apoprotein and the pigment molecule bacteriochlorophyll a. - pubmed
Although CT or MRI findings of adrenal histoplasmosis have been documented , there are no reports regarding 18F - fluorodeoxyglocose ( FDG ) positron emission tomography ( PET ) findings . - pubmed
The deduced amino acid sequences encoded by the cDNAs contained characteristic conserved elements of vacuolar invertases , including the sequence R[G / A / P]xxxGVS[E / D / M]K[S / T / A / R ] , located in the prepeptide region , Wxxx[M / I / V]LxWQ , located around the starting site of the mature protein , and an intact beta - fructosidase motif . - pubmed
Chemical double mutant cycles have been used to measure the effect of a range of substituents on face - to - face stacking interactions with phenyl and pentafluorophenyl rings . - pubmed
Previous data show that flow stimulates SHP2 translocation to cell membranes and binding to phosphotyrosine proteins . - pubmed
Flow - induced ERK1 / 2 phosphorylation depends on SHP2 phosphatase activity and SHP2 binding to phospho - PECAM1 ( platelet endothelial adhesion molecule 1 ) , suggesting that SHP2 forms a signaling module with PECAM1 . - pubmed
SHP2 - associated proteins were analyzed by SDS - PAGE and identified by mass spectrometry . - pubmed
Flow significantly increased tyrosine phosphorylation of both Tie2 and PECAM1 and their association with SHP2 . - pubmed
The results show that ACTH , but not corticosterone , responses to novel restraint are lower in chronically cold stressed females compared to non - stressed control females but no effect of 5 - HT-3A receptor deletion was observed . - pubmed
The absence of the PsaC subunit in the photosystem I ( PSI ) complex ( native PSI complex ) by mutagenesis or chemical manipulation yields a PSI core ( P700 - F(X ) core ) that also lacks subunits PsaD and PsaE and the two iron - sulfur clusters F(A ) and F(B ) , which constitute an integral part of PsaC. - pubmed
Sphingosylphosphorylcholine and lysophosphatidylcholine are ligands for the G protein - coupled receptor GPR4 . - pubmed
Although protein expression did not consistently match mRNA levels , a role for the two cell cycle regulators in the IFN - alpha signaling pathway is suggested as well as a relation with the resistance to IFN - alpha or imatinib therapy . - pubmed
Laboratory findings showed renal dysfunction ( creatinine clearance , 52 ml / min ) , a moderate range of urinary protein ( 1.51 g / day ) , and increased serum lysozymes ( 20.7 microg / ml ; normal range , 3.4 - 8.6 microg / ml ) . - pubmed
Renal biopsy revealed immune complex glomerulonephritis ( IgM deposition type ) with a membranoproliferative pattern , without granuloma or calcium deposition . - pubmed
The mechanism of DNA cytosine-5 - methylation catalyzed by the bacterial M.HhaI enzyme has been considered as a stepwise nucleophilic addition of Cys-81 - S- to cytosine C6 followed by C5 nucleophilic replacement of the methyl of S - adenosyl - L - methionine to produce 5 - methyl-6 - Cys-81 - S-5,6 - dihydrocytosine . - pubmed
The pH - dependent titration of wild - type reveals a spectrum characteristic of family I aminotransferases with an aldimine pK(a ) of 7.22 . - pubmed
I249A mutant hTATase exhibits an unusual spectrum with a similar aldimine pK(a ) ( 6.85 ) . - pubmed
hTATase has very narrow substrate specificity with the highest enzymatic activity for the Tyr / alpha - ketoglutarate substrate pair , which gives a steady state k(cat ) value of 83 s(-1 ) . - pubmed
The present findings show that hTATase is the only known aminotransferase that discriminates significantly between Tyr and Phe : the k(cat)/K(m ) value for Tyr is about four orders of magnitude greater than that for Phe . - pubmed
A comparison of substrate specificities of representative Ialpha and Igamma aminotransferases is described along with the physiological significance of the discrimination between Tyr and Phe by hTATase as applied to the understanding of the molecular basis of phenylketonuria . - pubmed
Acetylcholinesterase inhibitors for Alzheimer ' s disease : anti - inflammatories in acetylcholine clothing ! - pubmed
These observations are supported by evidence showing a role for acetylcholine in suppression of cytokine release through a ' cholinergic anti - inflammatory pathway ' . - pubmed
Taken together , these results indicate that inulin intake ameliorates PB - induced liver injury , associated with a decline in lipid accumulation and PB - induced expression of CYP2B and CYP3A , which may be related by a reduction in the nuclear expression of HNF4alpha . - pubmed
The surface properties of treated ITO substrates were studied by atomic force microscopy ( AFM ) , X - ray photoelectron spectroscopy ( XPS ) , sheet resistance , contact angle , and surface energy measurements . - pubmed
Osteopontin protects the islets and beta - cells from interleukin-1 beta - mediated cytotoxicity through negative feedback regulation of nitric oxide . - pubmed
Osteopontin ( OPN ) , a phosphorylated glycoprotein that binds to an integrin - binding motif , has been shown to regulate nitric oxide ( NO ) production via inhibition of induced NO synthase ( iNOS ) synthesis . - pubmed
In the transplanted islets , iNOS and toxic amounts of NO are produced as a result of islets infiltration with inflammatory cells and production of proinflammatory cytokines . - pubmed
OPN promoter activity was increased in the presence of IL-1beta , IL-1beta - induced NO , and an inducer of NO synthesis . - pubmed
These data suggest the presence of a cross talk between the IL-1beta and OPN pathways and a unique trans - regulatory mechanism in which IL-1beta - induced NO synthesis feedback regulates itself through up - regulation of OPN gene transcription . - pubmed
OBJECTIVES : We investigated the effects of the orally bioavailable non - immunosuppressive immunophilin ligand GPI 1046 ( GPI ) on erectile function and cavernous nerve ( CN ) histology following unilateral or bilateral crush injury ( UCI , BCI , respectively ) of the CNs . - pubmed
Intra - operative quick insulin assay to confirm complete resection of insulinomas guided by selective arterial calcium injection ( SACI ) . - pubmed
PMA and cytokines stimulate binding of C - Jun , C - Fos , CCAAT / enhancer binding protein beta ( C / EBPbeta ) , or NF - kappaB to their respective regulatory elements on COX-2 promoter . - pubmed
To further investigate the cellular effects of these drugs in the context of bone healing , we examined the effects of COX-1 inhibitor indomethacin and COX-2 inhibitors , parecoxib and NS398 on osteoclast and osteoblast differentiation and activity in vitro . - pubmed
We discovered that all tested COX - inhibitors significantly inhibited osteoclast differentiation , by 93 % , 94 % and 74 % of control for 100 microM indomethacin , 100 microM parecoxib and 3 microM NS398 , respectively . - pubmed
As this was an integrated reactor / analyzer , the GC column was exposed to temperatures beyond the degradation point of the column , and so selective ion storage RF waveform was used to remove the siloxane masses from the spectra . - pubmed
OBJECTIVES : To determine if ghrelin and adipocytokine ( leptin , adiponectin , resistin ) levels vary with menopause stage or with estradiol ( E2 ) , testosterone ( T ) , follicle - stimulating hormone ( FSH ) and sex hormone - binding globulin ( SHBG ) concentrations measured in three stages of the menopause transition . - pubmed
Downregulation of BST-2 requires both the transmembrane / ion channel domain and conserved serines in the cytoplasmic domain of Vpu . - pubmed
Treatment with SN-38 for 24 h suppressed TS protein expression and this low level of TS was maintained for at least 72 h. Pretreatment with SN-38 inhibited the 5 - FU - induced increase of TS protein . - pubmed
The synergistic effect induced by the combination of SN-38 and 5 - FU may be attributable to the SN-38 - induced suppression of TS protein . - pubmed
Furthermore , uracil and 5 - chloro-2,4 - hydroxypyridine , which are clinically available dihydropyrimidine dehydrogenase inhibitors , enhanced 5 - FU - induced growth inhibition . - pubmed
This may be the reason why Tk - GK is not inhibited by fructose 1,6 - bisphosphate , because the fructose 1,6 - bisphosphate binding site is produced only when a tetrameric structure is formed . - pubmed
Anticoagulant surface modification of titanium via layer - by - layer assembly of collagen and sulfated chitosan multilayers . - pubmed
In the present work , collagen ( Col ) and sulfated chitosan ( SCS ) multilayers were coated on pure titanium using a layer - by - layer ( LBL ) self - assembly technique . - pubmed
Platelet adhesion in vitro , partial activated thromboplastin time ( APTT ) and prothrombin time ( PT ) assays were used to evaluate the hemocompatibility of the Col - SCS multilayer coated titanium . - pubmed
An agonist - antagonist interaction model for prolactin release following risperidone and paliperidone treatment . - pubmed
A competitive agonist - antagonist interaction model described the competition between these drugs and dopamine for the D ( 2 ) receptors that regulate prolactin release . - pubmed
Tolerance development was explained by a feedback loop with prolactin stimulating dopamine release , whereas models wherein tolerance is described in terms of depletion of a prolactin pool did not explain the data well . - pubmed
It is originally indicated that the key mediator of the cholinergic anti - inflammatory pathway , acetylcholine ( ACh ) , inhibits cytokine release directly via the alpha7 nicotinic ACh receptor ( nAChR ) expressed on macrophages . - pubmed
OTX2 may play a role in development of the retinal pigment epithelium and photoreceptor differentiation , whereas OTX1 may be important in ciliary body development . - pubmed
By in situ hybridization and immunohistochemistry , we show that PAX6 was primarily localized to nuclei of the neural retina , OTX2 was localized to the nuclei of retinal pigment epithelium and OTX1 expression was confined to anterior retina . - pubmed
Using cell lines and measurement procedures to isolate the effects on individual membrane transport proteins , crofelemer at 50 microM had little or no effect on the activity of epithelial Na (+ ) or K (+ ) channels or on cAMP or calcium signaling . - pubmed
Crofelemer inhibited the cystic fibrosis transmembrane regulator ( CFTR ) Cl (- ) channel with maximum inhibition of approximately 60 % and an IC(50 ) approximately 7 microM. - pubmed
Crofelemer action at an extracellular site on CFTR produced voltage - independent block with stabilization of the channel closed state . - pubmed
Crofelemer did not affect the potency of glycine hydrazide or thiazolidinone CFTR inhibitors . - pubmed
Crofelemer action resisted washout , with & lt;50 % reversal of CFTR inhibition after 4 h. Crofelemer was also found to strongly inhibit the intestinal calcium - activated Cl (- ) channel TMEM16A by a voltage - independent inhibition mechanism with maximum inhibition & gt;90 % and IC(50 ) approximately 6.5 microM. - pubmed
In this paper , we investigate by Surface Plasmon Resonance ( SPR ) if captavidin , a recently described biotin - binding regenerable protein , could be used to bind and release biotinylated biocomponents for the development of regenerable biosensors . - pubmed
Because pyrazinoic acid is the active moiety , it should also increase overall pyrazinamide activity and , because most resistance arises in the pncA gene that converts pyrazinamide to pyrazinoic acid , it should act on most pyrazinamide resistant strains . - pubmed
On multivariable analysis , maximum , average , or standard deviation of glucose values predicted OS and maximum or average glucose values predicted nonrelapse mortality ( NRM ) . - pubmed
The oxysterol 27 - hydroxycholesterol increases beta - amyloid and oxidative stress in retinal pigment epithelial cells . - pubmed
Levels of Abeta peptide , mitochondrial and endoplasmic reticulum ( ER ) stress markers , Ca2 + homeostasis , glutathione depletion , reactive oxygen species ( ROS ) generation , inflammation and cell death were assessed using ELISA , Western blot , immunocytochemistry , and specific assays . - pubmed
RESULTS : 27 - OHC dose - dependently increased Abeta peptide production , increased levels of ER stress specific markers caspase 12 and gadd153 ( also called CHOP ) , reduced mitochondrial membrane potential , triggered Ca2 + dyshomeostasis , increased levels of the nuclear factor kappaB ( NFkappaB ) and heme - oxygenase 1 ( HO-1 ) , two proteins activated by oxidative stress . - pubmed
CONCLUSIONS : The cholesterol metabolite 27 - OHC is toxic to RPE cells . - pubmed
The deleterious effects of this oxysterol ranged from Abeta accumulation to oxidative cell damage . - pubmed
The CP and the CMT markedly decrease in the presence of NaCl due to the dehydration of the polyethylene oxide shell . - pubmed
[ Granulocyte colony stimulating factor - producing spindle cell renal cell carcinoma successfully treated by chemotherapy consisting of gemcitabine and doxorubicin ] . - pubmed
Nafion coating the ferrocenylalkanethiol and encapsulated glucose oxidase electrode for amperometric glucose detection . - pubmed
This paper describes the fabrication and application of a complex electrode -- Nafion film coating ferrocenylalkanethiol ( FcC ( 11 ) SH ) and encapsulated glucose oxidase ( GOD ) on a gold electrode . - pubmed
There were no significant amino acid mutations of the hemagglutinin ( HA ) and neuraminidase ( NA ) sites of the viruses , including the receptor binding , glycosylation , antigenic and catalytic sites and NA inhibitor targets . - pubmed
All the viruses had polybasic amino acids at the HA cleavage site . - pubmed
Platelet response to agonists ( TRAP , collagen , ADP ) and to hypotonic shock decreased , CD62P expression increased in both storage media over time . - pubmed
These data suggest that HCQ , a common medication used to treat RA , possesses beneficial effects upon insulin sensitization . - pubmed
This result was associated with increased sFlt-1 , VEGF receptor-2 ( VEGFR-2 ) , VEGFR-3 and endoglin levels but decreased NO metabolites . - pubmed
MgSO4 therapy ameliorated systolic hypertension and proteinuria and decreased sFlt-1 , VEGFR-2 , VEGFR-3 and endoglin levels but increased NO metabolites in the treated group . - pubmed
RESULTS : ATO treatment induced up - regulation of some pro - apoptotic genes , such as HRK , BAK1 , CASPASE-5 , BAD , TNFRSF1A , and BCL2L14 and down - regulation of ICEBERG . - pubmed
CONCLUSIONS : These results represent the basis for a possible association of ATO with other biological compounds able to modify the apoptotic pathways , such as inhibitors of the BCL2 family . - pubmed
The base substitutions change a glycine to arginine in the fibronectin type 3 domain of nephrin and a proline to arginine in a conserved proline - rich region in Neph3 . - pubmed
Survival analysis to verify the impact of these gene polymorphisms on the clinical outcome of lung cancer showed that lung squamous cell carcinoma patients with the Thr / Met genotype at XRCC3 had a significantly shorter survival time than those with the Thr / Thr genotype ( 13 months versus 48 months ; log - rank test , p & lt ; 0.0001 ) . - pubmed
PPi hydrolysis by soluble inorganic pyrophosphatase ( iPPase ) has been considered as an important homeostatic mechanism . - pubmed
Furthermore , our results revealed that COE inhibited the transcriptional activity of MMP-9 by suppression of the binding activity of NF - kappaB in the MMP-9 promoter , and inhibited IkappaBalpha phosphorylation and nuclear translocation of NF - kappaB. - pubmed
CONCLUSIONS : COE inhibits IL-1beta and TNF - alpha combination - induced FLSs invasion by suppressing NF - kappaB - mediated MMP-9 expression . - pubmed
Immunocytochemisty demonstrated RPE - specific expression profiles in cells after 12 - weeks of co - culture on RWSF / PCL / Gt membranes . - pubmed
Methionine sulfoxide reductase ( MSR ) repairs oxidized Met and protects organisms from oxidative damage . - pubmed
We found that ZmMSR genes were induced by polyethylene glycol ( PEG ) and NaCl , both known to generate oxidative stress . - pubmed
( 99m ) Tc - MDP SPECT / CT as the one - stop imaging modality for the diagnosis of early setting of Kienbock ' s disease . - pubmed
( 99m ) Tc - Methylene diphosphonate ( MDP ) triple phase bone scintigraphy ( BS ) has a role in early diagnosis of Kienbock ' s disease , especially when the X - ray is negative . - pubmed
Well - defined depth profiles of delta ( 18 ) O , Al , and sea - salt - Na (+ ) allowed the determination of chronology of the snow pit that spanned approximately 6 years from spring 2003 to summer 2009 . - pubmed
Se concentrations were at a low pg / g level , ranging from 7.2 to 45 pg / g , and exhibited high variability with generally higher values during winter and spring and lower values during summer and fall . - pubmed
High Se / MSA ratios are generally observed in the winter and spring snow layers , in which the Se concentrations were relatively high ( & gt;20 pg / g ) . - pubmed
This study tests the hypothesis that preservation of GLP-1 by the GLP-1 receptor agonist liraglutide or the dipeptidyl peptidase-4 ( DPP-4 ) inhibitor linagliptin is associated with a reduction of angiotensin ( Ang ) II - induced cardiac fibrosis . - pubmed
Liraglutide ( 0.3 mg / kg ) was subcutaneously injected twice daily or linagliptin ( 8 mg / kg ) was administered via oral gavage daily during Ang II infusion . - pubmed
Liraglutide and linagliptin comparatively reduced the protein level of the Ang II AT1 receptor and up - regulated the AT2 receptor as identified by a reduced AT1 / AT2 ratio ( 0.4 + - 0.02 and 0.7 + - 0.01 vs. 1.4 + - 0.2 in control , p & lt ; 0.05 ) , coincident with the less locally - expressed AT1 receptor and enhanced AT2 receptor in the myocardium and peri - coronary vessels . - pubmed
Both drugs significantly reduced the populations of macrophages ( 16 + - 6 and 19 + - 7 vs. 61 + - 29 number / HPF in control , p & lt ; 0.05 ) and alpha - SMA expressing myofibroblasts ( 17 + - 7 and 13 + - 4 vs. 66 + - 29 number / HPF in control , p & lt ; 0.05 ) , consistent with the reduction in expression of TGFbeta1 and phospho - Smad2 / 3 , and up - regulation of Smad7 . - pubmed
Here , we show that pHnuc dynamics can be imaged using the DNA - binding dye Hoechst 33342 . - pubmed
Cardiac myocyte pHnuc changed in response to maneuvers that alter nuclear Ca ( 2 + ) signals . - pubmed
Blocking Ca ( 2 + ) release from inositol-1,4,5 - trisphosphate receptors stably alkalinized the nucleus . - pubmed
The results also showed a weak positive correlation between HbA1c and TSH ( r = 0.212 , P = 0.034 ) and insignificant correlation with thyroxin T4 ( r = -0.018 , P = 0.855 ) . - pubmed
There was a weak positive correlation between HbA1c and total cholesterol and low density lipoprotein ( r = 0.258 , P = 0.001 ) , ( r = 0.297 , P = 0.003 ) , respectively . - pubmed
CONCLUSION : It is concluded that increased blood glucose could trigger anterior pituitary gland to increase secretion of TSH , whereas there was no direct correlation between increased glycemic index and the rate of thyroxine secretion . - pubmed
Key strategies included providing alcohol - based handrub dispensers at points of care , designing educational programs tailored to the needs of different health care workers , placement of general and individual reminders in the workplace , and establishment of evaluation and feedback for HH compliance and infection rates . - pubmed
A combination of X - ray diffraction , thermal analysis , FTIR , observations of isotropic chemical shifts of ( 1 ) H in distinct solvents , their temperature dependence and spin - lattice relaxation time constants ( T1 ) , solution ( 1D and 2D ) ( 13 ) C nuclear magnetic resonance ( NMR ) , and solid - state ( 13 ) C NMR ( CPMAS NMR ) provides valuable structural information and structural elucidation of micronized EFZ and heptane - recrystallized polymorphs ( EFZ / HEPT ) . - pubmed
Following 24h of incubation , cells were fixed and stained for migration and fluorescence analyses and the supernatant collected for quantification of total protein , hydroxyproline , bFGF , IL-6 and IL-10 . - pubmed
Synthesis and Biological Evaluation of 1 - Methyl-1H - indole - Pyrazoline Hybrids as Potential Tubulin Polymerization Inhibitors . - pubmed
A series of 1 - methyl-1H - indole - pyrazoline hybrids were designed , synthesized , and biologically evaluated as potential tubulin polymerization inhibitors . - pubmed
Cryogenic transmission electron microscopy ( Cryo - TEM ) images showed a substantial number of liposomes in addition to cubosomes in the dispersion containing 4 - 1 ( w / w ) phytantriol - to - F127 prepared by the BU approach compared to very low liposome content with the TD approach . - pubmed
In control group the HK , PFK and ALD activities showed strong positive correlation with blood sugar level while PGI activity did not show any correlation . - pubmed
The LDH activity only in the control group showed positive correlation with marginal increase with increasing concentrations of glucose . - pubmed
The effect of 1,25 - dihydroxyvitamin D3 on TSLP , IL-33 and IL-25 expression in respiratory epithelium . - pubmed
1,25D3 increased TSLP concentration in unstimulated nasal epithelial cells , but did not influence IL-33 and IL-25 expression . - pubmed
Simultaneously 1,25D3 augmented IL-25 production in the same model of stimulation . - pubmed
We also found that physical activity levels negatively affect BMI ( -0.148 ) and positively affect high - density lipoprotein ( HDL ) ( 0.106 ) ; sociodemographic background negatively affects diet ( -0.522 ) and BMI ( -0.352 ) ; HDL positively affects total cholesterol ( 0.230 ) and has a negative effect on BMI ( -0.108 ) , triglycerides ( -0.128 ) and waist circumference ( -0.104 ) . - pubmed
BMI and HDL ( -0.002 ) together mediate the effect of physical activity on diabetes status and similarly HDL and tryglycerides ( -0.005 ) also mediate the effect of physical activity on diabetes status . - pubmed
ARII exhibits a photocycle including a quasi - equilibrium state of M , N , and O ( M N O ) at near neutral and above pH values . - pubmed
These photoproducts , termed O1 and O2 , may be even produced at physiological pH , although they are not clearly observed under this condition due to the existence of a long M - N - O equilibrium . - pubmed
Effects of Triflusal and Clopidogrel on the Secondary Prevention of Stroke Based on Cytochrome P450 2C19 Genotyping . - pubmed
Moreover , we directly demonstrated that nano - ZnO was adsorbed on the algal B - EPS and entered into the algal cells by transmission electron microscope coupled with energy dispersive X - ray ( TEM - EDX ) . - pubmed
Rolipram , a specific phosphodiesterase-4 inhibitor which is traditionally used as an anti - depressant drug , has been recently proven to exert neuroprotective effects in several central nervous system insults . - pubmed
Rolipram treatment remarkably promoted the expression of Sirtuin 1 ( SIRT1 ) while inhibited NF - kappaB activation . - pubmed
Moreover , rolipram significantly inhibited the activation of microglia as well as down - regulated the expression of pro - inflammatory cytokines TNF - alpha , IL-1ss , and IL-6 . - pubmed
In addition , rolipram increased the expression of protective cytokine IL-10 . - pubmed
The neuroprotective effects of rolipram were associated with regulating the SIRT1 / NF - kappaB pathway . - pubmed
Alcohol dehydrogenase ( ADH ) is the principal enzyme responsible for the metabolism of ethanol . - pubmed
The quantitative contributions of ADH isozymes and ethnically distinct allozymes to cellular ethanol metabolism remain poorly understood . - pubmed
The organ simulations indicate that in homozygous ADH1B * 1 /* 1 livers , a representative genotype among ethnically distinct populations due to high prevalence of the allele , major contributors at 1 to 10 mM ethanol are ADH1B1 ( 45 % to 24 % ) and the ADH1C allozymes ( 54 % to 40 % ) . - pubmed
The model simulations suggest that slightly higher or similar ethanol elimination rates for ADH1B * 2 /* 2 and ADH1B * 3 /* 3 individuals compared with those for ADH1B * 1 /* 1 individuals may result from higher hepatocellular acetaldehyde . - pubmed
FcalphaRI binding at the IgA1 CH2 - CH3 interface induces long - range conformational changes that are transmitted to the hinge region . - pubmed
FcalphaRI binds to the IgA1 - Fc domain ( Fcalpha ) at the CH2 - CH3 junction and , except for CH2 L257 and L258 , all side - chain contacts are contributed by the CH3 domain . - pubmed
The energetic contribution of each IgA residue in the binding interface was assessed by alanine - scanning mutagenesis and equilibrium surface plasmon resonance ( SPR ) . - pubmed
Surprisingly , individual mutation of CH2 residues L257 and L258 , found at the periphery of the FcalphaRI binding site , dramatically reduced binding affinity . - pubmed
Comparison of antibody : receptor complexes involving IgA or its precursor IgY revealed a conserved receptor binding site at the CH2 - CH3 junction ( or its equivalent ) . - pubmed
Given the importance of residues near the CH2 - CH3 junction , we used coarse - grained Langevin dynamics simulations to understand the functional dynamics in Fcalpha . - pubmed
Subsequent SPR experiments confirmed that FcalphaRI binding to the Fcalpha CH2 - CH3 junction altered the kinetics of HAA lectin binding at the IgA1 hinge . - pubmed
Compared to ApKir2 , ApKir1 mediated currents that were larger in magnitude , more sensitive to barium , and less inhibited by small molecule VU041 . - pubmed
Shogaol but not gingerol has a neuroprotective effect on hemorrhagic brain injury : Contribution of the alpha , beta - unsaturated carbonyl to heme oxygenase-1 expression . - pubmed
We investigated the effects of shogaol , which has an alpha , beta - unsaturated carbonyl group , and gingerol , which does not , on primary - cultured microglia to understand how the alpha , beta - unsaturated carbonyl interacts with Kelch - like ECH - associated protein ( Keap ) 1 . - pubmed
Shogaol ( 1 muM ) but not the same concentration of gingerol significantly increased heme oxygenase ( HO )- 1 protein levels in cultured microglia without cytotoxicity . - pubmed
In addition , shogaol suppressed the release of the inflammation marker nitric oxide induced by 30 U / ml thrombin treatment . - pubmed
A docking simulation suggested that the alpha , beta - unsaturated carbonyl of shogaol but not gingerol interacts with Keap1 . - pubmed
The transcription inhibitor actinomycin D ( 0.1 mug / ml ) markedly blocked the increase of HO-1 mRNA levels by shogaol . - pubmed
Intracerebroventricular injection of 0.2 nmol shogaol increased striatal HO-1 protein levels and rescued ICH - induced neuron loss . - pubmed
Thus , the alpha , beta - unsaturated carbonyl is necessary for the interaction of compounds , such as shogaol , with Keap1 , and these findings may be useful for screening novel ICH therapeutic agents that increase HO-1 expression . - pubmed
Nicotine functions as a discriminative stimulus in modulating operant behavior as well as Pavlovian feature stimuli in modulating the conditional responding ( CR ) to exteroceptive CS US contingencies . - pubmed
This experiment evaluated the interaction among Pavlovian feature positive ( FP ) and feature negative ( FN ) effects of nicotine on concurrently occurring operant SD and SDelta effects . - pubmed
For the other group ( n = 8) nicotine served as the SDelta ( lever pressing non - reinforced ) and FP for the CS , with saline serving in the reverse roles . - pubmed
Consecutive brief non - reinforcement tests revealed that : A ) rates of lever pressing were significantly greater in SD than SDelta with nicotine and saline suggesting strong operant discriminative stimulus control ; B ) FP responding to the light CS with nicotine and saline was evident ; and C ) FN suppression of the CR with nicotine was not evident but weak under saline . - pubmed
The heteroblastic switch optimizes carbon efficiency in the juvenile phase ( by sterile shoots ) in the mesic post - fire conditions . - pubmed
Blood glucose , insulin levels , lipid profiles , and oxidative stress markers were measured . - pubmed
Ca and dairy product intakes may be inversely associated with all - cause and cause - specific mortality , and non - Ca components of dairy products , such as insulin - like growth factor-1 , may be independently associated with mortality . - pubmed
The MM - PB(GB)SA ( molecular mechanics - Poisson Boltzmann / Generalized Born ) Surface Area ) binding free energies calculated using the Amber force fields bsc0 and OL15 correlate well with the NMR titration and binding affinity measurements , with both calculations correctly identifying the EPI as the strongest binder to the hybrid-2 telomeric G4 wtTel26 . - pubmed
We have also performed binding free energy calculation using the more rigorous double decoupling method ( DDM ) ; however , the results correlate less well with the experimental trend , likely due to the difficulty of adequately sampling the very large conformational reorganization in the G4 induced by the protoberberine binding . - pubmed
Here , we investigated the occurrence of 21 organochlorine pesticides ( OCPs ) , 14 current use pesticides ( CUPs ) , 47 halogenated flame retardants ( HFRs ) , and 19 organophosphate esters ( OPEs ) in air around Las Cruces ( LC ) and La Selva ( LS ) Biological Stations , Costa Rica , and Kibale National Park ( KNP ) , Uganda using passive air samplers ( PAS ) with polyurethane foam ( PUF ) discs ( PAS - PUF ) . - pubmed
In this paper , astrocytic calcium oscillations and neuronal firing dynamics are presented by De Pitta and IF ( Integrated & amp ; Fire ) models , respectively . - pubmed
This low - cost hardware architecture for the astrocyte model is able to show the essential features of different types of Ca2 + modulation such as amplitude modulation ( AM ) , frequency modulation ( FM ) , or both modes ( AFM ) . - pubmed
This new neuromorphic circuit of astrocyte is able to successfully demonstrate AM / FM / AFM calcium signaling in its real operation on FPGA and has applications in self - repairing systems . - pubmed
AxyA hydrolyzes isoprimeverose ( alpha - D - xylopyranosyl -( 1 6 )- D - glucopyranose ) into D - xylose and D - glucose and shows hydrolytic activity with other xyloglucan oligosaccharides such as XXXG ( heptasaccharide , Glc4Xyl3 ) and XLLG ( nonasaccharide , Glc4Xyl3Gal2 ) . - pubmed
The intracellular localization of AxyA may help determine the order of hydrolases acting on xyloglucan oligosaccharides . - pubmed
Fingolimod and Dimethyl - Fumarate - Derived Lymphopenia is not Associated with Short - Term Treatment Response and Risk of Infections in a Real - Life MS Population . - pubmed
Mean glucose values , insulin values given for correction per hospitalization , and the number of hypoglycemic events ( glucose < 70 mg / dL ) were documented . - pubmed
No significant differences were found regarding glucose level ( P = 0.462 ) and administered insulin units ( P = 0.639 ) . - pubmed
In addition , trastuzumab ( 10 muM ) induced SAN - PV conduction block and burst firings , which were blocked by wortmannin ( a PI3 K inhibitor , 100 nM ) . - pubmed
Settings and Design : Matched paired comparison between the TACE - apatinib and TACE alone group using 1:2 PSM was utilized . - pubmed
Towards integration of 64Cu - DOTA - trastuzumab PET - CT and MRI with mathematical modeling to predict response to neoadjuvant therapy in HER2 + breast cancer . - pubmed
After randomization , the first group ( mask then BVGA , n = 12 ) began breathing room air through the face mask , followed by 100 % O2 . - pubmed
By contrast , while breathing through a face mask , there were 8 of 25 ( 32 % ) and 5 of 25 ( 20 % ) cases of suspected leak in air and 100 % O2 , respectively ( air : p = 0.002 ; 100 % O2 : p = 0.014 by McNemar test ) . - pubmed
The standard molybdenum blue method , APHA 4500 PE has a detection limit of 30 mugL-1 phosphate ( 10 mugL-1 phosphorus ) in freshwater with a 5 cm cuvette . - pubmed
The second - most - soluble material , NiO D , and the third , NiO A , caused evident pulmonary inflammation , and the responses persisted for at least 91 days with collagen proliferation . - pubmed
A useful intravital sign of the formation of a postinfarction scar is provided by the examination of collagen metabolites -- oxyproline bound with collagen - like plasma protein and free serum oxyproline and urine excretion of total oxyproline . - pubmed
A sensitive , fluorometric method for the assay of microsomal hydroxylase ; N - demethylation of p - chloro - N - methylaniline . - pubmed
A peptide corresponding to the 11 amino acid residues of the NH2 - terminal portion in the sequence of carcinoembryonic antigen(CNTHETIC CEA ( 1 - 11 ) peptide was attached by means of a water - soluble carbodiimide reagent to multichain poly ( DL - alanine ( as well as to bovine serum albumin . - pubmed
3 Gastrin release and acid secretion produced by litorin was more rapid in onset but less intense and less sustained than that elicited by bombesin . - pubmed
4 Gall bladder contraction stimulated by litorin was probably caused by a double action of the peptide , directly on the bladder smooth muscle , and indirectly by cholecystokinin release . - pubmed
The immunosuppressive protocol included thymoglobulin during the induction period and a classical 3 - drug therapy , with a rapid tapering off of prednisone over 3 weeks . - pubmed
The rate of consumption of oxygen was 1.2 + /- 0.1 mmol / min , the respiratory quotient was close to unity , and close to half of the PDH was in its active form ; therefore , virtually all ATP synthesis should require flux through PDH . - pubmed
Both ACTH and cortisol exhibit complex secretory patterns demonstrating ultradian and circadian rhythms . - pubmed
Immunoreactive ACTH and cortisol were measured in all samples and glucose in selected samples . - pubmed
Plasma glucose was lower than preinsulin levels at 10 min , declined rapidly between 10 and 30 min , and remained low 30 - 60 min after insulin injection in both treatment groups . - pubmed
The increase in mean plasma hormone levels during hypoglycemia was predominantly due to an increase in amplitude of secretory pulses for ACTH and cortisol compared with the 30 min before insulin . - pubmed
Dexamethasone significantly lowered mean ACTH and cortisol levels and prevented alteration in plasma ACTH and cortisol secretion during hypoglycemia but did not totally ablate pulsatile activity of either hormone . - pubmed
The amplitude of ACTH and cortisol pulses was significantly decreased by dexamethasone treatment . - pubmed
The frequency of cortisol but not ACTH pulses was also significantly decreased. - pubmed
However , reduction of interleukin-2 ( IL-2 ) production and mitogen responses induced by GvHR were not rescued by prodigiosin 25 - C treatment . - pubmed
Prodigiosin 25 - C moderately prolonged survival of major histocompatibility ( MHC)-mismatched skin grafts . - pubmed
Nine different tissues were extracted with an acid acetate - buffer ( lung , heart , brain , adrenal , aorta , skeletal muscle , spleen and liver ) or with KSCN - buffer ( kidney ) , and antiplasmin activity was evaluated by a spectrophotometric method . - pubmed
The results indicate that an appropriate sympathetic neural environment is needed for cyclosporine to have an effect on ornithine decarboxylase activity in lymphoid tissue . - pubmed
The amino acid sequence deduced from the exons matches perfectly the one previously determined from the T - cell cyclophilin cDNA . - pubmed
The assignment of compartmental designation to the T2 component remains a problem except for homogeneous structures easily identifiable anatomically ( ventricles , superior sagittal sinus , globe of the eye ) . - pubmed
The predominance of the interstitial and plasma compartment , the invisibility of part of the intracellular sodium , and the difficulty in imaging the very fast T2 component of visible intracellular sodium make the usual Na spin - echo image essentially an image of the interstitial and plasma space . - pubmed
Unlike conventional barium studies CT is able to demonstrate completely the intussusception complex . - pubmed
Tissue patterns of gene expression were analyzed by measuring mRNA levels and incorporation of radioactive amino acids for cystatin C and beta 2 - microglobulin , the two extracellular proteins in the brain with the highest ratio of concentration in cerebrospinal fluid over that in blood plasma . - pubmed
The primary structure of rat cystatin C mRNA from choroid plexus was determined by nucleotide sequencing of cloned cDNA and the tissue patterns of gene expression were analysed by RNA blot analysis and in situ hybridization . - pubmed
Cystatin C was found to be composed of 120 amino acids and to contain a potential site for N - linked glycosylation . - pubmed
Choroid plexus pieces in culture secreted radioactive cystatin C when incubated with radioactive leucine . - pubmed
Changes in organ and glandular weights and serum thyrotropin levels reflected changes in serum thyroid hormone concentrations . - pubmed
In addition , the zinc - deficient state was found to be protective against thiouracil - induced suppression of the microsomal - monooxygenase and thyroxine-5 '- monodeiodinase enzyme complex . - pubmed
Effect of cimetidine on enzyme actuation .... - pubmed
The highly water - soluble , quaternarized derivatives of ellipticine have no bactericidal effect and do not induce the synthesis of rec A protein . - pubmed
Groups of four ganglia were incubated for 4 h with 20 nM [ 125I]alpha - bungarotoxin alone or with radioactive alpha - bungarotoxin plus either 1 microM unlabeled alpha - bungarotoxin or 100 microM d - tubocurarine . - pubmed
The EPR spectra of the SH - groups spin - labeled with maleimide or iodoacetamide show the existence of two populations of sulfhydryl groups , differing in their mobility ( one weakly , the other strongly immobilized ) . - pubmed
The phospholipase activity , subordinate to hypoxia , decreased by 1/5 compared to its value without treatment and the phospholipid composition of the broncho - alveolar lung lavage remained unchanged whereas after hypoxia without treatment the phosphatidylcholines level decreases by 26 % . - pubmed
We also showed that this effect could not be due to the only action of reduced sulphur : different concentrations of sulphur solutions had no action on the phospholipase A activity subordinate to hypoxia . - pubmed
This effect was demonstrable only in the presence of insulin and dexamethasone in cultural media . - pubmed
However , the use of monoclonal anti - Thy 1.2 antibodies failed to prove that antigen in question was structural virion protein or Thy 1.2 antigen . - pubmed
Effects of 5 - halopyrimidinones with antiviral and antineoplastic activity on murine cytochrome P-450 . - pubmed
These results suggest that 5 - halopyrimidinones can depress cytochrome P-450 levels and that this depression may affect the metabolism of other drugs by cytochrome P-450 . - pubmed
Sodium dodecyl sulfate - polyacrylamide gel electrophoresis analysis of the Sarkosyl - resistant proteins revealed a composition similar to that of the proteins exposed on the surfaces of viable cells , as determined by the lactoperoxidase-125I radioiodination method . - pubmed
In vitro , the diphosphate of azidocytidine was earlier shown to inhibit mammalian ribonucleotide reductase , while the triphosphate did not inhibit DNA replication in isolated cell nuclei . - pubmed
In intact cells , however , deoxyribonucleoside triphosphate pools were not decreased by azidocytidine in a manner expected from an inhibitor of ribonucleotide reductase . - pubmed
Azidocytidine was transformed to an active metabolite by phosphorylation with deoxycytidine kinase . - pubmed
By days 15 - -16 , dieldrin had reduced the pentobarbital sleep time by 54 % ( P & lt ; 0.05 ) , increased the liver mass by 8 % ( P & lt ; 0.05 ) , and increased hepatic microsomal cytochrome P-450 by 66 % ( P & lt ; 0.05 ) . - pubmed
Orally active and long - term acting insulin - mimetic vanadyl complex : bis ( picolinato ) oxovanadium ( IV ) . - pubmed
It is well known that excess amounts of antigen modulate TCR / CD3 complex on antigen - specific T lymphocytes . - pubmed
Single - base substitutions in the immediate 5'-flanking region of the fetal G gamma and A gamma globin genes have been associated with non - deletional forms of hereditary persistence of fetal haemoglobin ( HPFH ) . - pubmed
Previously , the sole promoter mutation associated with HPFH in British individuals has been the T to C substitution at position -198 relative to the A gamma globin gene CAP site . - pubmed
The mutation was first detected by examining the amplified 5 ' regions of both the G gamma and A gamma globin genes for heteroduplex formation after electrophoresis in a Hydrolink gel . - pubmed
The final step in the synthesis of PtdIns ( 4,5 ) P2 is the phosphorylation of PtdIns ( 4 ) P- by PtdIns ( 4 ) P 5 - kinase ( PIP5 K ) . - pubmed
STE4sd mutations resulted in amino acid substitutions in the N - terminal region of Ste4p , proximal to the first of seven repeat units conserved in G protein beta subunits . - pubmed
Wild - type cells that overexpressed STE4sd alleles displayed apparently normal initial responses to pheromone as judged by quantitative mating , G1 arrest and transcriptional assays . - pubmed
The entry criteria were serum total cholesterol ( TCHOL ) between 6.2 and 7.8 mM and low - density lipoprotein ( LDL ) cholesterol between 4.3 and 50 mM on a standard diet and after the 2 - week run - in period of placebo treatment . - pubmed
There was a significant linear dose response with regard to the decrease in LDL cholesterol , TCHOL , and triglycerides ( TG ) and the increase in high - density lipoprotein ( HDL ) cholesterol after 8 weeks of therapy . - pubmed
At each dose level , simvastatin reduced TCHOL and LDL cholesterol . - pubmed
Doses of simvastatin & gt ; or = 5 mg moderately increased HDL cholesterol and reduced serum TG . - pubmed
Substrate cycling from phosphoenolpyruvate to pyruvate [ via pyruvate kinase ( PK ) ] and from oxaloacetate to pyruvate [ via malic enzyme ( ME ) ] relative to the pyruvate carboxylase ( PC ) flux [ i.e. - pubmed
MARCKS specifically binds to phosphatidylserine but not phosphatidylcholine . - pubmed
The binding of MARCKS to phosphatidylserine was abolished on protein kinase C - dependent phosphorylation . - pubmed
These data suggest that phosphatidylserine is a membranous target molecule of MARCKS . - pubmed
Phosphoramidon and the neutral endopeptidase ( NEP ) inhibitors thiorphan and candoxatrilat blocked the vasoconstriction caused by big ET-1 in endothelium - denuded RSA . - pubmed
We have determined that the DNA sequence downstream of the well - characterized gonococcal fbp gene contains two open reading frames : one designated fbpB , which encodes a protein proposed to function as a cytoplasmic permease , and one designated fbpC , which encodes a protein proposed to function as a nucleotide - binding protein . - pubmed
Four isolates , previously shown to be unrelated to each other by pulsed - field gel electrophoresis analysis , were positive for both genes by PCR , although beta - lactamase production was not induced with methicillin . - pubmed
A methionine at amino acid position 11 of the HLA class II DP alpha 1 chain correlates with the occurring disease after infection ( relative risk 3.3 ) . - pubmed
The lack of adhesion molecule expression on CNS endothelium molecules may be an explanation for the poor leukocyte recruitment during Wallerian degeneration in CNS when compared with PNS . - pubmed
campestris in deduced amino acid sequence ) suggests that SLG diversification predates speciation . - pubmed
TPO induced tyrosine phosphorylation of platelet proteins was completely absent ( 3 of 4 ) or markedly decreased ( 1 of 4 ) . - pubmed
The catalytic effect of natural and non - natural amino acid substitutions at the P1 position in a 5A/5B peptide substrate was investigated . - pubmed
NS3 - NS4A demonstrated a marked catalytic preference for the cysteine residue commonly found in authentic substrates . - pubmed
A second class of inhibitors is NS4A - dependent and contains a natural or non - natural cyclic amino acid substituted for the commonly found P1 ' residue serine . - pubmed
Here we show that cholesterol feeding also reduces the levels of the nuclear NH2 - terminal domains of sterol regulatory element binding proteins ( SREBPs ) , which activate transcription of sterol - regulated genes . - pubmed
Cholesterol also appeared to reduce the proteolytic processing of SREBP-2 . - pubmed
In addition , at high levels of dietary cholesterol the mRNA encoding SREBP-2 declined and the amount of the precursor also fell , suggesting that cholesterol accumulation also may inhibit transcription of the SREBP-2 gene . - pubmed
The high - cholesterol diets reduced the amount of low density lipoprotein receptor mRNA by 30 % and produced a more profound 70 - 90 % reduction in mRNAs encoding 3 - hydroxy-3 - methylglutaryl CoA synthase and reductase . - pubmed
Treatment with lovastatin and Colestipol , which increases hepatic demands for cholesterol , increased the amount of SREBP-2 mRNA as well as the precursor and nuclear forms of the protein . - pubmed
The percentage of large B cells from the male BXSB that expressed CD40L was increased to 43 % by stimulation with LPS . - pubmed
However , resistance to commonly used antibiotics was very high , 94 % isolates were resistant to ampicillin which corresponds to the betalactamase production . - pubmed
Intravenous administration of H3 - receptor agonists significantly decreased noradrenaline release by the heart ( R - HA at 2 micromol kg(-1 ) h(-1 ): +77 + /- 25 vs +405 + /- 82 ; BP 2.94 at 1 mg kg(-1 ): +12 + /- 11 vs +330 + /- 100 pg ml(-1 ) in control conditions , P & lt ; or = 0.05 ) , and increases in heart rate ( R - HA at 2 micromol kg(-1 ) h(-1 ): +26 + /- 8 vs +65 + /- 10 and BP 2.94 at 1 mg kg(-1 ): +30 + /- 8 vs 75 + /- 6 beats min(-1 ) , in control conditions P & lt ; or = 0.05 ) , left ventricular pressure , and contractility . - pubmed
Pyridostigmine which is widely used as pretreatment of organophosphate poisoning protects cholinesterases of peripheral nervous system . - pubmed
Binding of lectin appeared strongest on the adluminal membrane of these cell populations suggesting a role for the carbohydrate moieties in nutrient transfer . - pubmed
Protease inhibitors , inhibitors of the endothelial cell proliferation , suppressors of angiogenic growth factors , copper chelators , and other compounds interfering with the process of angiogenesis were screened for inhibition of tumor angiogenesis and some of them are in clinical trials . - pubmed
PURPOSE : We review our technique of ureteroscopic management of lower pole renal calculi with Nitinol basket displacement and holmium laser stone fragmentation . - pubmed
RESULTS : The Nitinol device can be passed into the lower pole through the fully deflected ureteroscope without any loss of deflection . - pubmed
We also examined cells expressing this variant for sensitivity to the interstrand cross - linking agent , mitomycin C ( MMC ) , as HDR mutant cell lines , including the XRCC3 mutant , have been found to be hypersensitive to this DNA damaging agent . - pubmed
Exposure of erythrocytes to methylene blue shows the active role of catalase in removing hydrogen peroxide . - pubmed
Methylene blue ( MB ) is a powerful reducing agent that is widely used in clinical practice as well as for metabolic studies of the erythrocyte . - pubmed
In the presence of MB , catalase underwent inactivation even with the co - existence of active generation of NADPH , leaving the glutathione concentration unaffected . - pubmed
The data obtained in the present investigation show , using a different tool ( MB ) , that catalase is the active enzyme in H2O2 detoxification and that its integrity is largely dependent on an adequate generation of NADPH . - pubmed
Stimulation with testosterone of cells grown in depleted medium or exposed to tamoxifen , resulted in the aggregation of the expanded androgen receptor into characteristic cytoplasmic inclusions . - pubmed
Differential modulation of polyglutamine - expanded androgen receptor aggregation by ligands in neuronal cells may partly explain the absence of an overt disease phenotype in female carriers of the SBMA mutation and define conditions to explore further the role of androgen receptor aggregation in the pathogenesis of SBMA . - pubmed
As a result , antibody titers detected by PA were in parallel to the titers which detected by Nt test , and it was concluded that the differences in antibody titers would arise from the differences of test principles and viral antigens . - pubmed
Data obtained with the fpRFDR - CT , DQCSA , and 2D MAS exchange solid - state NMR techniques , which provide independent constraints on the phi and psi backbone torsion angles between the labeled carbonyl sites , indicate non - beta - strand conformations at G25 , S26 , and G29 . - pubmed
Cortisol treatment had no apparent effect on placental mRNA expression for the glucose transporters , GLUT-1 and GLUT-3 . - pubmed
Active peptides also activated protein kinase B and extracellular signal - regulated kinase ( ERK ) in vivo in a wortmannin - sensitive manner while reducing recoverable cellular p85 levels . - pubmed
Prolactin releasing peptides modulate background firing rate and milk - ejection related burst of oxytocin cells in the supraoptic nucleus . - pubmed
Existence of prolactin releasing peptide - immunoreactive cells in the dorsomedial nucleus and synaptic contact of prolactin releasing peptide - immunoreactive terminals with oxytocin cells was reported . - pubmed
Experiments were done to test the effect of prolactin releasing peptide on the electrical activity of oxytocin cells in the supraoptic nucleus . - pubmed
These results suggest that prolactin releasing peptide may modulate electrical activity of oxytocin cells not through its direct action on oxytocin cells but through its action on area other than supraoptic nucleus . - pubmed
It is conceivable that Tg polymorphisms , combined with the explosive mix of iodine , TPO and H2O2 necessary for thyroid hormone synthesis , inadvertently provide the trigger for the autoimmune thyroid response . - pubmed
The inhibition of caspases that target the amino acid sequence DXXD abrogated calnexin cleavage in apoptotic stimulus - treated cells . - pubmed
The obstacle facing Exo + polymerases for single nucleotide polymorphism ( SNP ) detection could be bypassed by using primer-3'-termini modification . - pubmed
ADP - ribosyl cyclase couples to cyclic AMP signaling in the cardiomyocytes . - pubmed
ADP - ribosyl cyclase ( ADPR - cyclase ) produces a Ca ( 2 +)-mobilizing second messenger cyclic ADP - ribose ( cADPR ) from beta - NAD (+ ) . - pubmed
In this study , we examined the molecular basis of which beta - adrenergic receptor ( betaAR ) stimulation induces cADPR formation and characterized cardiac ADPR - cyclase . - pubmed
Moreover , incubation of ventricular lysates with isoproterenol , forskolin or cAMP resulted in activation of ADPR - cyclase that was inhibited by pretreatment with H89 . - pubmed
Characterization of partially purified cardiac ADPR - cyclase showed a molecular mass of approximately 42 kDa and no cross - activity with CD38 antibodies , and the enzyme activity was inhibited by Zn ( 2 + ) but not dithiothreitol . - pubmed
Istradefylline , a novel adenosine A2A receptor antagonist , for the treatment of Parkinson ' s disease . - pubmed
1 - Substituted pyrazolo [ 1,5 - c ] quinazolines as novel Gly / NMDA receptor antagonists : synthesis , biological evaluation , and molecular modeling study . - pubmed
A new set of 5,6 - dihydro - pyrazolo [ 1,5 - c ] quinazoline-2 - carboxylates ( 2 - 18 ) , bearing different substituents ( COOEt , Cl , Br , CH ( 3 ) , and COOH ) at position-1 , were synthesized in order to investigate the influence of various groups at this specific position on Gly / NMDA receptor affinity and/or selectivity . - pubmed
The results obtained in this study have highlighted that a C-1 lipophilic substituent on the pyrazolo [ 1,5 - c ] quinazoline-2 - carboxylate core shifts selectivity toward the Gly / NMDA receptor , while a C-1 anionic carboxylate residue is able to increase affinity toward this receptor subtype . - pubmed
Furthermore , the 1,2 - dicarboxylic acids 13 and 14 are endowed with the highest Gly / NMDA receptor binding activity ( K(i)=0.09 and 0.059muM , respectively ) , among the pyrazoloquinazoline series of derivatives . - pubmed
Administration of secretin for autism alters dopamine metabolism in the central nervous system . - pubmed
In addition , we investigated the association between improvement in symptoms and changes in the cerebrospinal fluid ( CSF ) homovanillic acid ( HVA),5 - hydroxyindole-3 - acetic acid ( 5 - HIAA ) , and 6R-5,6,7,8 - tetrahydro - L - biopterin ( BH ( 4 ) ) levels after administration . - pubmed
These findings suggest that secretin activated metabolic turnover of dopamine in the central nervous system via BH(4 ) , improving symptoms . - pubmed
As the conditions of successful use of the NLS properties to enhance gene transfer are not clear , and with the aim of detecting and defining the requirements of NLS - enhanced transfection , we investigated gene delivery and expression into various cell lines with various DNA complexes ( lipoplexes or polyplexes ) that were formulated for various N / P ratios from various preformed Acr - spacer - NLS / DNA complexes ( 1:1 , 5:1 and 10:1 molar ratio ) . - pubmed
The peripherally restricted mu - opioid receptor ( MOR ) antagonist naloxone methiodide dose - dependently attenuated the effects of systemic morphine . - pubmed
BACKGROUND : CYP2C9 is one of the major drug metabolizing enzymes for many drugs including warfarin , NSAIDs and losartan . - pubmed
Diethylstilbestrol inhibits the proton pump and the passive H (+ ) conductance with about the same time course , at concentrations that have no effect on cytoplasmic streaming . - pubmed
The vitrification solution contained 40 % ethylene glycol , 18 % Ficoll , and 0.3 M sucrose in PBS . - pubmed
The embryos were placed for 1 to 2 min in vitrification solution ( Group 1 ) or following exposure to 20 % ethylene glycol in PBS for 10 to 20 min ( Groups 2 and 3 ) . - pubmed
Straws were warmed in water ( 20 degrees C , 20 sec ) , and the contents were expelled with 0.5 M sucrose in PBS . - pubmed
The activity of milin is inhibited by serine proteases inhibitors like PMSF , APMSF and DFP , but not by any other protease inhibitors such as E-64 and PCMB . - pubmed
The enzyme is a glycoprotein with detectable carbohydrate moiety ( 7 - 8 % ) in its constitution , which is essential for the activity . - pubmed
The numbers of tryptophan , tyrosine and cysteine residues in the sequence of milin were estimated chemically and are 23 , 14 and 14 , respectively . - pubmed
ChREBP binding to fatty acid synthase and L - type pyruvate kinase genes is stimulated by glucose in pancreatic beta - cells . - pubmed
The relative importance of the transcription factors carbohydrate - responsive element binding protein ( ChREBP ) , sterol - responsive element binding protein-1c ( SREBP-1c ) , and upstream stimulatory factor ( USF ) in the induction of lipogenic genes by glucose remains unclear . - pubmed
Both ChREBP and SREBP-1c were required for the induction of the fatty acid synthase ( FAS ) promoter by glucose , and chromatin immunoprecipitation ( ChIP ) assay revealed that glucose induced the binding of both ChREBP and SREBP-1c to the FAS promoter without affecting USF2 binding . - pubmed
By contrast , ChIP assay revealed that high glucose prompted direct binding of ChREBP , but not SREBP-1c or USF2 , to the liver - type pyruvate kinase ( L - PK ) promoter . - pubmed
This event was indispensable for the induction of the L - PK gene by glucose , as demonstrated by RNA silencing , single - cell promoter analysis , and quantitative real - time PCR . - pubmed
Identification of a protein hydrolysate responsive G protein - coupled receptor in enterocytes . - pubmed
Although such a role for GPCRs has been implicated in the intestinal response to protein hydrolysate , no GPCR directly involved in this process has been previously identified . - pubmed
In the present study , for the first time , we identified GPR93 expression in enterocytes and demonstrated its activation in these cells by protein hydrolysate with EC50 of 10.6 mg / ml as determined by the induction of intracellular free Ca2 + . - pubmed
In enterocytes , GPR93 was synergistically activated by protein hydrolysate in combination with an agonist , oleoyl - l - alpha - lysophosphatidic acid ( LPA ) , which activated the receptor in these enterocytes with EC50 of 7.9 nM. - pubmed
The increased intracellular Ca2 + by GPR93 activation was observed without the addition of a promiscuous Galpha protein and was pertussis toxin sensitive , which suggests Galpha ( q )- and Galpha ( i )-mediated pathways . - pubmed
Both nuclear factor of activated T cells and 12 - O - tetradecanoylphorbol 13 - acetate responsive elements reporter activities were induced by protein hydrolysate in cells exogenously expressing GPR93 . - pubmed
The peptidomimetic cefaclor by itself did not activate GPR93 but potentiated the protein hydrolysate response and further amplified the synergistic enhancement of GPR93 activation by protein hydrolysate and LPA . - pubmed
Insulin did not affect its serotonin content , whereas serotonin increased its insulin content at each concentration studied ( down to 10(-21)M ) . - pubmed
We determined if serotonin , glutamate decarboxylase ( GAD67 ) , or the kappa opioid receptor ( KOR ) were differentially located within these cell types as predicted by previous studies . - pubmed
Fixed sections were processed for detection of biotinamide and immunfluorescence either for serotonin or for KOR and GAD67 . - pubmed
The product analysis using HPLC , fluorimetry , and MS revealed the formation of thiol and nitric oxide as the major end products . - pubmed
The study of the interactions between these oligonucleotides and ACTD at 25 degrees C showed the formation of an interaction complex with a stoichiometry of 1:1 ( ACTD : duplex ) and values for the log(formation constant ) of 5.1+/-0.3 , 6.4+/-0.2 , and 5.6+/-0.2 , respectively . - pubmed
The aim of this study was to investigate whether L - Cys supplementation prevents modulation of the activities of erythrocyte membrane acetylcholinesterase ( AChE ) , Na (+) , K (+)- ATPase and Mg ( 2 +)- ATPase induced by free radicals in basketball players during training . - pubmed
Na(+),K (+)- ATPase activity was also higher post - exercise ( 1.27+/-0.05 micromol Pi / h x mg protein ) than that pre - exercise ( 0.58+/-0.04 micromol Pi / h x mg protein , p<0.001 ) . - pubmed
When the players were supplemented with L - Cys , both AChE and Na(+),K (+)- ATPase activities remained unaltered post - exercise . - pubmed
Endothelial progenitor cells stimulate cerebrovascular production of prostacyclin by paracrine activation of cyclooxygenase-2 . - pubmed
The increased production of PGI ( 2 ) and arterial content of cAMP were inhibited only by a selective cyclooxygenase-2 inhibitor , NS-398 . - pubmed
In vitro or in vivo treatment of basilar artery with conditioned media from EPCs also caused increase in cyclooxygenase-2 and PGI ( 2 ) synthase protein expression associated with elevation of cAMP . - pubmed
This effect appears to be mediated by activation of arachidonic acid metabolism via stimulation of cyclooxygenase-2 / PGI ( 2 ) synthase pathway . - pubmed
The median ( interquartile range ) of the highest total bilirubin level was 18 ( 15.8 - 20 ) mg / dL and 18.4 ( 16.3 - 19.5 ) mg / dL in normal G6PD and G6PD - deficient newborns , respectively ( p - value equals 0.7 ) . - pubmed
Cultured bacteria differentially expressed IpLA7 in response to changes in temperature , pH and concentration of 4,5 - dihydroxy-2,3 - pentanedione , the precursor of autoinducer 2 , indicative of mechanisms governing IpLA7 expression . - pubmed
This phosphatase was characterized as a marker for cellular stimulation by its high correlations with induced changes of extracellular pH : 10microM nigericin ( correlation coefficient r=0.91 ) , 100microM salicylic acid ( r=0.84 ) , heat shock 5min 37 degrees C ( r=0.79 ) , and heat shock after pre - treatment with 5microM fusicoccin ( r=0.92 ) or 0.5 % ethanol ( r=0.90 ) . - pubmed
The Golgi apparatus inhibitor Brefeldin A ( 200microM ) reduced the heat - shock - induced phosphatase ( -33 % ) . - pubmed
Thus , heat - shock - induced phosphatase was characterized by alkaline activity with inhibitors ( 10microM molybdate : -52 % , 2.5mM phosphate : -64 % , 10microM ZnCl ( 2 ): -82 % ) , substrates ( 2.5mM , tyrosine phosphate : 255pkat g(-1 ) , p - nitrophenyl phosphate : 92pkat g(-1 ) , serine phosphate : 0 , threonine phosphate : 0 ) , Hill coefficient ( nH=1.4 ) indicating two binding sites , and the extent of heat - shock stimulation ( p - nitrophenyl phosphate : +190 % , tyrosine phosphate : +180 % ) . - pubmed
SDS - PAGE showed a correlation of alkaline phosphatase with the heat - shock - induced release of highly N - glycosylated 53kDa protein , detected by peroxidase - labeled concanavalin A affinoblotting after endoglycosidase H treatment . - pubmed
The 53kDa protein showed no in - gel phosphatase activity after SDS - PAGE and regeneration treatment , in contrast to a putative dimer ( 105kDa ) . - pubmed
Leading models propose that the reduction in photosynthesis at elevated temperature is a function of either declining capacity of electron transport to regenerate RuBP , or reductions in the capacity of Rubisco activase to maintain Rubisco in an active configuration . - pubmed
It is proposed that measurement of the initial slope of the CO ( 2 ) response of photosynthesis ( the A / C(i ) response ) can be a useful means to screen for Rubisco activase limitations . - pubmed
This is because a reduction in the Rubisco activation state should be most apparent at low CO ( 2 ) when Rubisco capacity is generally limiting . - pubmed
[ A patient with early Alzheimer ' s disease who showed improvement of cognitive function and cerebral perfusion by combined therapy of nilvadipine and PPAR gamma agonists ] . - pubmed
Nilvadipine ( a Ca - channel blocker ) , telmisartan ( an angiotension II receptor blocker ) , and pioglitazone ( an insulin sensitizer ) were administered for the control of the hypertension and diabetes . - pubmed
Body condition score was greatest for MB and NM , least for HF , and intermediate for NRF , MBX , and NMX . - pubmed
The NRF survived the longest in the herd , the HF survived the shortest , and the NM , MB , MBX , and NMX were intermediate . - pubmed
Effects of tryptophan microenvironment , soluble domain , and vesicle size on the thermodynamics of membrane protein folding : lessons from the transmembrane protein OmpA. - pubmed
Wild - type OmpA exhibits a free energy of unfolding ( DeltaG degrees H2O ) of 10.5 kcal / mol . - pubmed
Mutants , containing a single tryptophan residue at the native positions 7 , 15 , 57 , 102 , or 143 , are less stable than wild - type OmpA , with DeltaG degrees H2O values of 6.7 , 4.8 , 2.4 , 4.7 , and 2.8 kcal / mol , respectively . - pubmed
The effect of the soluble tail on the conformational stability of the transmembrane domain of OmpA was also investigated via truncated single - Trp mutants ; DeltaG degrees H2O values for four of the five truncated mutants are greater by & gt;2.7 kcal / mol relative to the full - length versions , suggesting that the absence of the soluble domain may destabilize the unfolded transmembrane domain . - pubmed
LC - MS and NMR spectroscopic studies demonstrate that the process involves a pyrone tetraene ( 10 ) as key intermediate , whereas the levels of the final polypropionates ( 6 , 7 and 9 ) are related to each other and show a significant dependence upon light conditions . - pubmed
TRPs can be grouped into 7 main subfamilies according to structural homology : the TRPC ( canonical ) , TRPV ( vanilloid ) , TRPM ( melastatin ) , TRPP ( polycystin ) , TRPML ( mucolipin ) , TRPA ( ankyrin ) , and TRPN ( NO mechanopotential ) . - pubmed
In the present study , we tested whether leptin can protect gut epithelial cells from methotrexate ( MTX)-induced intestinal damage . - pubmed
The AUC after oral glucose was related to ethnicity , age and triglycerides ( r 2=0.27 ) ; after WB to ethnicity , age , triglycerides , sex and CRP ( r 2=0.43 ) ; after CCC to age and weight ( r 2=0.18 ) ; and after FL to age and CRP ( r 2=0.12 ) . - pubmed
Activity of creatine kinase ( CK ) , lactate dehydrogenase ( LDH ) , myeloperoxidase ( MPO ) superoxide dismutase ( SOD ) and level of malondialdehyde ( MDA ) in plasma and culture medium were measured to assess the degree of injury and inflammation of myocardial tissues and cells . - pubmed
RESULTS : Calcium channel blockers ( verapamil , diltiazem and nifedipine ) significantly attenuated myocardial injury , as shown by reduced release of CK and LDH , preserved SOD activity and decreased MDA production and MPO activity . - pubmed
Thinning or dehiscence of the superior semicircular canal may occur on the middle cranial fossa floor or adjacent to the superior petrosal sinus ( SPS ) . - pubmed
Levels of HbA and HbB , Hb and hemopexin , a transporter of heme , were measured . - pubmed
Hemin increased HbA , HbB and hemopexin mRNA levels in cultured neurons . - pubmed
Deferoxamine reduced hemin - induced neuronal Hb expression . - pubmed
ICH increased HbA and HbB expression in the brain , which may potentially serve to buffer the heme released during clot resolution . - pubmed
Desmosine ( DES ) and isodesmosine ( IDES ) are both pyridinium amino acid isomers that serve as cross - linking molecules binding the polymeric chains of amino acids into elastin . - pubmed
In this study , we aimed to evaluate the resistin levels in premature neonates and the effect of events such as preterm prelabour rupture of the membranes ( PPROMs ) and the use of antenatal steroids on these levels . - pubmed
Babies with PPROMs had significantly higher levels of resistin ( ( n=30 ) ; 70.7 ( 7.8 to 568.4 ) ng ml(-1 ) ) than babies without PPROM ( ( n=88 ) ; 25.9 ( 5.5 to 528.9 ) ng ml(-1 ) ) ( P=0.005 ) , and the babies of mothers who received antenatal steroids had significantly lower resistin levels ( ( n=44 ) ; 20.8 ( 5.5 to 159.9 ) ng ml(-1 ) ) than the babies of mothers who did not ( ( n=66 ) ; 34.6 ( 7.2 to 568.4 ) ng ml(-1 ) ) ( P=0.015 ) . - pubmed
There were significant correlations between resistin and IL-6 levels and between IL-6 and procalcitonin and CRP levels in babies whose mothers did not receive antenatal steroids . - pubmed
Elevated resistin levels in babies with PPROM and suppressed levels in babies whose mothers received antenatal steroids reported in this study might have been observed as a result of the effects of fetal inflammation on resistin levels . - pubmed
Mutations in the Plasmodium falciparum chloroquine ( CQ ) resistance transporter ( PfCRT ) are major determinants of verapamil ( VP)-reversible CQ resistance ( CQR ) . - pubmed
In western blot analysis , the expression of cleaved caspase-3 and heat shock protein 70 declined in Estradiol pretreated group compared to ischemic control group . - pubmed
Interestingly , Estradiol respectively increased and decreased the expression of Cyclooxygenase ( COX )- 1 and COX-2 , to a significant extent . - pubmed
As for compounds affecting endocannabinoid levels in living tissues , many classes of selective and , in some cases , drug - like inhibitors of FAAH are available , while only compounds with poor selectivity or in vivo activity are known to inhibit other enzymes involved in endocannabinoid catabolism , such as NAAA or MGL , and in endocannabinoid biosynthesis . - pubmed
Mitochondrial targeting by use of lipid nanocapsules loaded with SV30 , an analogue of the small - molecule Bcl-2 inhibitor HA14 - 1 . - pubmed
The screen also identified broad - spectrum importin alpha / beta inhibitors such as ivermectin , which may represent useful tools for nuclear transport research in the future . - pubmed
The authors validate the activity and specificity of mifepristone and ivermectin in inhibiting nuclear protein import in living cells , underlining the utility of the screening approach . - pubmed
influenzae were collected and beta - lactamase - negative isolates were screened for reduced ampicillin susceptibility using an ampicillin 2 mug disc ( breakpoint & lt;17 mm ) and Etest ( breakpoint & gt;=0.25 mg / L ) . - pubmed
All screen - positive isolates had their ampicillin MICs determined by reference broth microdilution and their ftsI genes were sequenced . - pubmed
Enantiomeric , ferromagnetically coupled decanuclear { Ni6Fe4 } cages with adamantane - like cores were synthesized around templating tetraethylammonium cations , as shown by crystallographic analysis and CSI - MS , and their homochiral nature was confirmed by circular dichroism measurements . - pubmed
The multiple branching was achieved by breaking the face - centered - cubic lattice symmetry of gold through copper - induced formation of single or double twins , and the resulting gold nanocrosses exhibited pronounced near - IR absorption with a great extension to the mid - IR region . - pubmed
GLYX-13 , a GluN2B - preferring , NMDAR glycine - site partial agonist ( 1 mg / kg , i.v . ) - pubmed
significantly increased positive emotional learning whereas the GluN2B receptor - specific antagonist , ifenprodil ( 10 mg / kg , i.p . ) - pubmed
Using the RAFT polymerization technique in combination with the reactive ester approach , here we have synthesized well - defined and narrowly distributed N -( 2 - hydroxypropyl ) methacrylamide homopolymers ( pHPMA ) ( P1 * and P2 * ) and random HPMA copolymers consisting of hydrophilic HPMA and hydrophobic lauryl methacrylate comonomers ( P3 * and P4 * ) . - pubmed
Western blot analysis followed by quantitative analysis of respective immunoblot data for AANAT protein , radioimmunoassay of melatonin , and spectrophotometric analysis of Ca ( 2 + ) in the pineal revealed stimulatory effects of both adrenergic ( alpha ( 1 ) and beta ( 1 ) ) and dopaminergic ( D(1 ) ) agonists and cholinergic ( both nicotinic and muscarinic ) antagonists , inhibition by both adrenergic and dopaminergic antagonists and cholinergic agonists , but independent of the influence of any agonists or antagonists of alpha ( 2 )- adrenergic receptors . - pubmed
Band intensity of AANAT and concentration of melatonin in the pineal were also enhanced by the intracellular calcium - releasing agent , activators of both calcium channel and adenylate cyclase , and phophodiesterase inhibitor , but suppressed by inhibitor of calcium channel and adenylate cyclase as well as activator of phophodiesterase . - pubmed
Moreover , an inhibitory effect of light on the pineal AANAT and melatonin was blocked by both cAMP and proteasomal proteolysis inhibitor MG132 . - pubmed
Cytokine levels were determined in blood and saliva using ELISA , three times after the administration of methotrexate and only once in the control group . - pubmed
RESULTS : The following statistically significant differences were seen : control group pre- vs post - treatment wave I - IV interval values ( p & lt ; 0.05 ) ; control vs vitamin E and B groups ' I - IV interval values ( p & lt ; 0.05 ) ; control vs other groups ' hearing thresholds ; vitamin E vs vitamin B and C and L - carnitine groups ' hearing thresholds ( p & lt ; 0.05 ) ; and vitamin B vs vitamin C and L - carnitine groups ' hearing thresholds ( p & lt ; 0.05 ) . - pubmed
Kinetics of advanced glycation end products formation on bovine serum albumin with various reducing sugars and dicarbonyl compounds in equimolar ratios . - pubmed
Comparing different concentrations of BSA , we applied various reducing sugars and reactive dicarbonyl compounds as AGE - inducing agents at different concentrations . - pubmed
The NACE - MS procedure was successfully applied for the analysis of real samples from seizures in illegal fentanyl laboratories . - pubmed
We found that 3 - month L - arginine supplementation resulted in significant decrease of HOMA - IR and insulin concentration . - pubmed
Since the depolarization is initiated by the inhibition of a TASK - like K (+ ) channel , we studied if CIH may increase the amplitude of the hypoxic - induced depolarization in the chemoreceptor cells , due to an enhanced inhibition of the TASK - like current . - pubmed
Present results show that acute hypoxic inhibition of TASK - like K (+ ) channel is potentiated by CIH exposure , suggesting that the enhancing effect of CIH on CB chemosensory responsiveness to hypoxia occurs at the initial step of the oxygen transduction in the CB chemoreceptor cells . - pubmed
The COMT rs4680 Met / Met genotype was associated with higher impulsivity on the BIS-11 second - order factor Non - planning . - pubmed
The results suggest that a reduction in anthocyanin concentration causes the MRB - G sport . - pubmed
Transcript levels of PcUFGT ( for UDP - glucose : flavonoid 3 - O - glucosyltransferase ) , the key structural gene in anthocyanin biosynthesis , paralleled the change of anthocyanin concentration in both MRB and MRB - G fruit . - pubmed
The infiltrated fruits showed blocked anthocyanin biosynthesis , higher methylation of the PcMYB10 promoter , and lower expression of PcMYB10 and PcUFGT . - pubmed
Vitamin D exerts its effect through vitamin D receptor ( VDR ) . - pubmed
Our data suggest that single nucleotide polymorphisms ( SNPs ) in FokI may be possibly involved in longevity and cognitive function . - pubmed
The purpose of our investigation was to compare the effect of RYGB , vertical sleeve gastrectomy ( VSG ) , and duodenal switch ( DS ) on insulin and glucose response to carbohydrate challenge . - pubmed
Homoeostatic Model Assessment ( HOMA)-IR , fasting to 1 - h and 1- to 2 - h ratios of glucose and insulin , were calculated . - pubmed
All operations caused weight loss ( BMI 47.7 + - 10 - 30.7 + - 6.4 kg / m(2 ) in RYGB ; 45.7 + - 8.5 - 31.1 + - 5.5 kg / m(2 ) in VSG ; 55.9 + - 11.4 - 27.5 + - 5.6 kg / m(2 ) in DS ) , reduction of fasting glucose , and improved insulin sensitivity . - pubmed
CONCLUSIONS : Compared to gastric bypass , DS results in greater weight loss and improves insulin sensitivity and glucose homeostasis without causing a hyperinsulinemic response . - pubmed
DPMA inhibited microtubule formation and induced expression of Bax , cleaved caspase-3 , p53 and ROS , and inhibited Bcl-2 expression . - pubmed
DPMA also affected cyclinB1 , cdc2 and p - cdc2 expression , inducing cell cycle arrest . - pubmed
These studies demonstrate that DPMA inhibits p53 / cdc2 / Bax signaling , thereby inhibiting cell growth / angiogenesis and inducing apoptosis . - pubmed
In contrast , mutant p62 expression in OCL precursors promotes receptor activator of NF - kappaB ligand ( RANKL ) hyperresponsivity and increased OCL production , but OCLs that form have normal morphology , are not hyperresponsive to 1,25 - dihydroxyvitamin D3 ( 1,25 -( OH ) 2 D3 ) , nor produce elevated levels of IL-6 . - pubmed
The main outcome measures were most preferred concentration of salt in broth and crackers ; most preferred concentration of sucrose in water and jelly ; reported dietary intake of salty and sweet foods ; levels of a bone growth marker ; anthropometric measurements such as height , weight , and percent body fat ; and TAS1R3 ( sweet taste receptor ) genotype . - pubmed
The neurosteroid allopregnanolone ( AP ) is a GABAA receptor agonist that influences fetal brain development and has neuroprotective properties . - pubmed
Co - administration of the AP analogue analog alfaxalone with BM prevented this change in MBP expression . - pubmed
The structure - activity relationship ( SAR ) of these arylpiperazine derivatives was also discussed based on the obtained experimental data . - pubmed
They showed resistance or intermediate susceptibility to expanded - spectrum cephalosporins , other beta - lactams , a beta - lactam / beta - lactamase inhibitor combination , and gentamicin . - pubmed
ALA concentration and lipid peroxidation in post - thawed sperm was higher in all treated groups when compared to the control group . - pubmed
Alkylating agent melphalan augments the efficacy of adoptive immunotherapy using tumor - specific CD4 + T cells . - pubmed
We showed that melphalan treatment resulted in a rapid burst of inflammatory cytokines and chemokines during the cellular recovery phase after melphalan - induced myelodepletion and leukodepletion . - pubmed
After melphalan treatment , tumor cells exhibited characteristics of immunogenic cell death , including membrane translocation of the endoplasmic reticulum - resident calreticulin and extracellular release of high - mobility group box 1 . - pubmed
These findings provide mechanistic insights into melphalan 's immunostimulatory effects and demonstrate the therapeutic potential of combining melphalan with adoptive cell therapy utilizing antitumor CD4(+ ) T cells . - pubmed
In addition , multiple linear regression analysis revealed that ABCG2 rs2231142 had a remarkable effect on LTG concentrations which is stated to be 4.8 % of the variability of LTG and may also help to interpret ethnic difference in LTG pharmacokinetics . - pubmed
MK-801 ( 100 muM ) , a specific non - competitive antagonist to NMDA - receptor complex , prevented this effect . - pubmed
Thus , depending on concentration , NMDA can inhibit the synaptic transmission in Schaffer collaterals - hippocampal CA1 pyramidal neurons axis without triggering the epileptiform activity . - pubmed
D - AP5 ( 50 muM ) , a competitive antagonist to NMDA - receptor complex , completely prevented the inhibitory effect of NMDA ( 40 muM ) . - pubmed
Thus , MK-801 can prevent the inhibitory action of NMDA on synaptic transmission in Schaffer collaterals - hippocampal CA1 pyramidal neurons axis via blocking the channel of NMDA - receptor complex , while NMDA exerts its effect only via activation of NMDA receptors . - pubmed
Folylpolyglutamate synthetase splicing alterations in acute lymphoblastic leukemia are provoked by methotrexate and other chemotherapeutics and mediate chemoresistance . - pubmed
Anti - HBs antibody development rate by HB vaccination was similar between HBcrAg - positive and negative patients ( p = 0.325 ) . - pubmed
The results showed that ABA concentrations in seeds were low during early development and increased later , peaking at about 50 DAP . - pubmed
ABA concentrations in rin accession were similar to those of the control cultivar and thus are not directly associated with the occurrence of vivipary . - pubmed
SSEA-1 is a marker that detects malignant thyroid neoplasms in formalin - fixed paraffin - embedded thyroid tissue sections and may be a useful marker for thyroid cancer stem - like cells . - pubmed
The conversion of arginine to citrulline residues on histones is catalyzed by peptidylarginine deiminase 4 ( PAD4 ) . - pubmed
Here we demonstrate that Cl - amidine treatment prior to CLP improves overall survival in sepsis and the abrogation of PAD4 has minimal effects on the proinflammatory immune response to sepsis , while it has no effect on overall neutrophil migration to the peritoneum . - pubmed
In addition , both Si and Cu ions were released from the Cu - BGN in a sustained manner for at least 14days in cell culture medium , indicating the potential of the BGN as promising carriers for delivering therapeutic Cu ions . - pubmed
Release of the glucose - regulating hormone insulin from pancreatic islet beta cells is critical for whole - body metabolic homeostasis . - pubmed
Recent insights indicate a convergence of metabolism - driven signals , such as lipid - derived messengers and redox - dependent deSUMOylation , at the plasma membrane to augment Ca2 + -dependent insulin exocytosis . - pubmed
( ChemRU330 / PPRI 13195 ) had the highest relative enzyme activity and was tested along with isolates belonging to Hyloscyphaceae ( EdRU083 / PPRI 17284 ) and Leotiomycetes ( EdRU002 / PPRI 17261 ) for endoglucanase production under different pH and nutritional conditions that included : carbon sources , nitrogen sources and metal ions , at an optimum temperature of 28 C. An optimal of pH 5.0 produced enzyme activity of 3.99 , 2.18 and 4.31 ( U / mg protein ) for isolates EdRU083 , EdRU002 and Leohumicola sp . - pubmed
Increased enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as potassium , sodium , glucose , maltose , cellobiose , tryptone and peptone . - pubmed
The catalytic activity of these NSs towards the PSCB was followed up by thermogravimetric analysis ( TGA ) , where they increased the percentage of total weight loss , and lowered the decomposition temperatures of PSCB . - pubmed
Depupylase Dop Requires Inorganic Phosphate in the Active Site for Catalysis . - pubmed
Analogous to eukaryotic ubiquitination , proteins in actinobacteria can be post - translationally modified in a process referred to as pupylation , the covalent attachment of prokaryotic ubiquitin - like protein Pup to lysine side chains of the target protein via an isopeptide bond . - pubmed
However , the enzymes involved in pupylation and depupylation have evolved independently of ubiquitination and are related to the family of ATP - binding and hydrolyzing carboxylate - amine ligases of the glutamine synthetase type . - pubmed
Here , we investigate the role played by the nucleotide in the active site of the depupylase Dop using a combination of biochemical experiments and X - ray crystallographic studies . - pubmed
Evidence is presented for a mechanism where the inorganic phosphate acts as the nucleophilic species in amide bond cleavage and implications for Dop function are discussed . - pubmed
Observed average concentrations of PM2.5 , CO , percent relative humidity ( % RH ) and temperature ( T ) in five different kitchen types were 549.6mug / m3 , 4.2ppm , 70.2 % and 20 C respectively . - pubmed
Urinary prednisolone excretion is a determinant of serum hepcidin levels in renal transplant recipients . - pubmed
PURPOSE : Recent reports indicate that histamine and its novel , high - affinity histamine H4 receptor ( H4R ) play a role in carcinogenesis , and thus H4R signalling has become a focus of increasing interest in the pathogenesis of many cancers . - pubmed
In the periplasm , Fdh2 and hydrogenase Hyn play an important role in reverse electron transport associated with succinate oxidation . - pubmed
The sulfate reduction pathway was poorly regulated and many enzymes associated with sulfate reduction ( Sat , HppA , AprAB , DsrAB and DsrC ) were abundant even at conditions where sulfate was not present . - pubmed
Hdr / Flox was detected in all conditions while HdrABC / HdrL was exclusively detected when sulfate was available ; these complexes most likely confurcate electrons . - pubmed
Nitric oxide ( NO ) production was also significantly suppressed in a dose - dependent manner ( P & lt ; 0.05 ) with an IC50 , the phosphorylation level of JNK of 3.5 microg / mL. - pubmed
In the anti - inflammatory pathway studies , ERK , p38 and IkappaBalpha were also decreased by shikonofuran E at 10 muM , in spite of the total levels of the MAPK isoforms and IkappaBalpha did not differ significantly . - pubmed
Amino acid substitution in the C - terminal domain of collagen XVII reduces laminin-332 interaction causing mild skin fragility with atrophic scarring . - pubmed
Indeed , the pR1303Q collagen XVII ectodomain showed decreased binding capability to laminin-332 and was less co - localized with pericellular laminin-332 molecules in cell culture . - pubmed
Compared to high - level serum zinc group , the risk of SGA ( OR ( 95%CI ) in low - level serum zinc group during first trimesters was 1.51 ( 1.05 - 2.19 ) ) . - pubmed
Density functional theory and MP2 theoretical approximations predict the existence of two conformers , syn - gauche and syn - anti , depending on the value adopted by the dihedral angles phi ( Cl - C - O - S ) and phi ( C - O - S - Cl ) . - pubmed
Early conservative therapy such as bed rest , legs massage , low fat diet with medium chain triglycerides , diuretics , bronchodilator inhaler was not be able to satisfy us ( chylous out put & gt ; 330 cc / d ) . - pubmed
Therefore the catheter replacement with chest tube was carried out followed by pleurodesis using Talc and doxycycline besides transition of oral intake to total parentral nutrition and vitamine E supplement , on post - operative day = 41 . - pubmed
Two experimental dentifrices with the same ingredients but different SnF2 concentrations ( Group II , 0.4 % w / w ; Group III , 0.454 % w / w ) were used ; distilled water was used in the control group ( Group I ) . - pubmed
Neuroprotective Effect of Natural Alkaloid Fangchinoline Against Oxidative Glutamate Toxicity : Involvement of Keap1 - Nrf2 Axis Regulation . - pubmed
The protective effects of FAN were almost completely antagonized by inhibitor of Nrf2 . - pubmed
Subsequent studies revealed that FAN could down - regulate Kelch - like ECH - associated protein 1 ( Keap1 ) in both mRNA level and protein level . - pubmed
To sum up , our result demonstrated the protective effects of FAN against glutamate - induced oxidative neuronal damage , and for the first time clarified the anti - oxidative mechanisms of FAN involve activating endogenous antioxidant defense system including enhancing SOD activity and regulating Keap1 / Nrf-2 antioxidation signaling through modulation of Keap1 expression . - pubmed
The X - ray crystallographic study confirmed that the title compound crystallizes in the monoclinic P21 / c space group and possesses a bridging butyl - ene group that connects two identical phenyl tetra - zole moieties . - pubmed
Especially , cloquintocet , as a TP of cloquintocet - mexyl , had a concentration ratio TP / parent = 14,809 in effluent . - pubmed
Survival analyses showed that low PNI was an independent predictor of short time to treatment failure ( TTF ) [ hazard ratio ( HR ) = 6.87 , P=0.0052 ] , and high NLR ( HR = 3.53 , P=0.0375 ) and high mGPS ( HR = 23.2 , P=0.0038 ) were independent prognostic factors for overall survival ( OS ) after atezolizumab . - pubmed
Vascular endothelial growth factor and pigment epithelial - derived factor in the peripheral response to ketamine . - pubmed
There was a significant group x time interaction for VEGFA mRNA levels ( F(1 , 21 ) = 5.207 , p = 0.029 ) , with ketamine increasing VEGFA levels . - pubmed
There was no significant effect of either ketamine or midazolam on PEDF levels . - pubmed
There was a significant group x time interaction for VEGFA / PEDF mRNA ratio , with ketamine alone increasing this ratio ( F(1 , 11 ) = 12.085 , p = 0.005 ) . - pubmed
CONCLUSIONS : These preliminary results support a role for VEGF in the action of ketamine and suggest a novel role for VEGF / PEDF in the molecular response to ketamine . - pubmed
Here we estimate maximal in vivo catalytic rates for 168 plant enzymes , including photosystems I and II , cytochrome - b6f complex , ATP - citrate synthase , sucrose - phosphate synthase as well as enzymes from amino acid synthesis with previously undocumented enzyme kinetic data in BRENDA . - pubmed
Binary Logistic regression analysis of SUA / Cr and MS and its components correlation were also performed . - pubmed
A restricted cubic spline model was used to analyze the dose - response relationship between SUA / Cr and MS morbidity . - pubmed
SUA / Cr positively correlated with the number of MS components(P<0 . - pubmed
The correlation intensity between SUA / Cr value and MS showed a linear dose - response relationship in euthyroid population(P<0 . - pubmed
Next , the oxazoline ring could be broken via the nucleophilic attacking of a water molecular at C1 position , which generates the final product . - pubmed
Primary aldosteronism causes secondary erythropoiesis through activation of the renin - aldosterone system , but the mechanism is not clear . - pubmed
Red blood cells ( RBC ) were incubated with the fluorescent Laurdan probe and median values of Generalized Polarization ( GP ) , representative of membrane fluidity , were compared between the two groups . - pubmed
After recovery , no differences were observed between Tris and ACP -116c that provided 515.4 x 106 sperm / mL and 561.6 x 106 sperm / mL , presenting > 65 % motile sperm , respectively . - pubmed
Seven patients ( 26.9 % ) had a TE result > = 7.1kPa , which was associated with higher triglyceride levels , FIB4 score and CAP values . - pubmed
An alternative explanation was suggested by the finding that inhibition of locomotion by aggregated IgG was dependent on the presence of divalent cations at the time the IgG was added , and did not occur in the presence of the calcium ionophore A23187 . - pubmed
Addition of aggregated IgG or chemotactic factors to lymphocytes thus may lead to a gated entry of calcium , and following closure of the calcium gate , the cells become relatively unresponsive to further stimulation . - pubmed
Provisions were made to differentiate ME from LE by the use of cyanogen bromide ( CNBr ) to destroy methionine , the carboxy terminal amino acid in the ME sequence . - pubmed
A comparison of the affinities of eukaryotic initiation factor 2 and eukaryotic elongation factor 1 for GTP and GDP , and of the responses of initiation and elongation complex formation to various GTP mol fractions indicated that the initiation reaction was more sensitive to changes in the GTP : GDP ratio . - pubmed
Unexpected occurrence of an aminoacylated nucleoside in mammalian tRNATyr . - pubmed
Each wave is characterized by the emergence , from a pool of growing follicles , of a large dominant follicle which reaches a mature stage of development and may be induced to ovulate and form a functional corpus luteum with a single treatment of either human chorionic gonadotrophin ( hCG ) or gonadotrophin - releasing hormone ( GnRH ) . - pubmed
Such thyroid antigen - specific T cells have now been shown to recognize one or other of the three major thyroid - specific antigens ; thyroglobulin , thyroid peroxidase , or the TSH receptor and efforts are currently in progress to characterize the T - cell epitopes of these major thyroid autoantigens . - pubmed
There is compelling evidence that the thyroid cell itself , by expressing HLA molecules , and presenting antigen directly to the T cells , may initiate disease , perhaps after an external insult . - pubmed
Long - term reduction of vasopressin excretion induced by the central injection of an immunoconjugate ( antibody to vasopressin linked to ricin A chain ) . - pubmed
In order to investigate the increase in both duration and amplitude of the biological effects seen after the injection of vasopressin - monoclonal antibody , an immunoconjugate was constructed with the vasopressin - monoclonal antibody IgG1k isotype and the cytotoxic part of the ricin molecule , the ricin A chain . - pubmed
When non - specific IgG replaced specific antibody , vasopressin excretion , diuresis as well as urine osmolality were unchanged . - pubmed
However , it acts subsequent to retinoic acid , and at least one early retinoic acid - induced event is unaltered in the presence of LIF . - pubmed
The events occurring during mammary epithelial cell reorganization on the matrix were shown to be mediated by cell surface beta-1,4 - galactosyltransferase ( GalTase ) , a receptor that binds N - acetylglucosamine residues on glycosylated proteins . - pubmed
The protein alpha - lactalbumin ( alpha - LA ) inhibits the association of GalTase with N - acetylglucosamine . - pubmed
Cells cultured on plastic , which lack surface GalTase activity , were unperturbed by incubation with alpha - LA . - pubmed
Between nucleotide position 116343 and 116344 two nucleotides -AG- are inserted which prolong the ORF of the UL56 gene to 233 amino acids with a predicted molecular weight of 30 kDa . - pubmed
HFI patients exhibit a deficiency of fructose 1 - phosphate aldolase ( aldolase B ) , the isozyme expressed in tissues that metabolize fructose . - pubmed
The viability of the cells was assessed biochemically [ cellular catecholamine content and the catalytic activities of tyrosine hydroxylase ( TH ) and lactate dehydrogenase ( LDH ) ] and anatomically ( by electron microscopy ) . - pubmed
When cultures of BAMC cells were exposed to MPTP or MPP + for 3 days , a marked loss of cellular catecholamines and TH activity was observed . - pubmed
The addition of an inhibitor of monoamine oxidase ( MAO ) B ( Ro 19 - 6327 ) , but not MAO A ( clorgyline ) , prevented the toxicity of MPTP but not that of MPP + . - pubmed
The toxicity of MPP + was time dependent , with losses of TH and the release of cellular LDH occurring after 48 h in culture . - pubmed
The rate of glucose consumption was markedly accelerated by MPP + treatment and the losses in cell TH and the release of LDH into the media were preceded by a 99 % depletion of glucose from the media . - pubmed
Culturing cells in glucose - free medium greatly potentiated the effects of MPP + on cellular TH and catecholamines . - pubmed
Lithium ( Li ) was shown in therapeutic doses to be a specific inhibitor of the enzyme inositol monophosphate esterase Li reduces the turnover of PIP2 and diminishes the cell responses . - pubmed
In order to investigate this new mode of action of Li the authors studied the influence of acute dose of Li on the response of prolactin ( PRL ) and thyrotropin ( TSH ) to thyrotropin stimulating hormone ( TRH ) , a stimulation mainly mediated through the activation of the turnover of PIP2 . - pubmed
One such suppressor , MSI1 , was found to encode a putative protein of 422 amino acids that shows homology to the beta subunit of the mammalian guanine nucleotide - binding regulatory proteins . - pubmed
Analysis of the psoralen - crosslinking pattern in chromatin DNA by exonuclease digestion . - pubmed
The pattern of these crosslinks can be analysed by exonuclease digestion of random DNA fragments , since the exonucleases tested stop at sites of psoralen - crosslinks . - pubmed
Further digestion of these fragments with S1 - nuclease leads to DNA fragments of nucleosomal and polynucleosomal size , which presumably carry psoralen - crosslinks at both ends . - pubmed
The assay for UR phosphorylase appeared to be comparable to existing methods with radiolabeled UR as substrate regarding sensitivity and linearity . - pubmed
Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens . - pubmed
Serum lysozyme and vitamin B 12 binding capacity in myeloproliferative disorders . - pubmed
However , the ratio of [ 14C ] DMA - labelled hemoglobin in the sol phase to that in the gel phase in the unfractionated hemolysate was 1.17 at pH 7.4 and 1.25 at pH 8.4 , suggesting that part of the cross - linked hemoglobin tetramers was incorporated into the gel . - pubmed
In experiment II the unit activity of both cortical areas was compared during the free head movement towards food and during the movement hampered with the rubber thread . - pubmed
There are several possible explanations for the preincubation effects , such as new synthesis of carrier protein , altered fluidity of the membrane or stimulation of the sodium - dependent taurocholate uptake via a change in the cation distribution . - pubmed
Since the development peak eluted ahead of the vegetative peak in all but one case , and since levels of amino acid acceptance and tRNA chromatographic profiles were otherwise quite similar , it was suggested that a common change or a very small number of changes in base modification were occurring during development . - pubmed
Indeed , the erythromycin - resistance marker ( erm ) , originating in pAM beta 1 , has been used as an indicator of local transcription on several occasions . - pubmed
To overcome this limitation , new drug - resistance cassettes were designed and constructed , using pA as a model for synthetic promoters for the erm and cat genes . - pubmed
Erythrocyte filterability ( 5 microns pore size ) was significantly lower , fibrinogen concentration significantly higher , and HDL - cholesterol concentration significantly lower in the patient group . - pubmed
No significant differences were found regarding hematocrit , mean corpuscular volume , hemoglobin concentration , leukocyte count and filterability ( 8 microns pore size ) , plasma viscosity , and total cholesterol concentration . - pubmed
Selective binding of gelsolin to actin monomers containing ADP . - pubmed
The rate of reaction and the stoichiometry of binding between gelsolin and actin monomers depends on adenine nucleotides . - pubmed
In the presence of Ca2 + but not Mg2 + , gelsolin retains the ability to sever actin filaments when incubated for more than 20 min with an excess of G - actin in the presence of ATP but loses severing activity within seconds when mixed with G - actin in ADP . - pubmed
Immunoprecipitation of gelsolin removes more actin from ADP than from ATP solutions . - pubmed
Monomeric ATP - actin in 2 mM MgCl2 and 150 mM KCl slowly destroys the filament - severing activity of gelsolin with kinetics that are first order in actin concentration and with an apparent bimolecular rate constant of 0.021 + /- 0.007 microM-1 s-1 . - pubmed
Coincident with the slow complex formation in MgCl2 , the actin bound to the calcium - sensitive actin binding domain of gelsolin hydrolyzes its ATP to ADP . - pubmed
These results suggest a further level of gelsolin regulation and a functional similarity between actin and GTP - binding proteins . - pubmed
Each of the exon products 7 ( Lys236 - Gln292 ) , 9 ( Val293 - Gly328 ) , and 8 ( Gln329 - Met357 ) , and their combinations were evaluated for the ability to inhibit thrombin induced platelet aggregation . - pubmed
A deletion mutant of exon 7 product , polypeptide 7A product ( Lys236 - Lys270 ) did not block thrombin - induced platelet aggregation , while 7B product ( Thr255 - Gln292 ) and 7C product ( Leu271 - Gln292 ) inhibited aggregation . - pubmed
Peptides Phe279 - Ile283 and Phe281 - Gln292 did not block thrombin , and Asn275 - Phe279 had only minimal inhibitory activity . - pubmed
A heptapeptide Leu271 - Ala277 inhibited thrombin - induced aggregation of platelets with an IC50 of 65 microM. - pubmed
The effect is specific for the activation of platelets by thrombin but not ADP or collagen . - pubmed
G - CSF - treatment significantly shortened the optimal time interval of CPA doses when compared with treatment with the control vehicle . - pubmed
These results suggest that G - CSF shortens the optimal time interval between CPA doses not only by accelerating the recovery of G - CFC but also by affecting the hemopoietic environment . - pubmed
DNA sequence determination of the fusion joints revealed an open reading frame ( ORF1 ) , encoding a gene product of 137 amino acids with a typical N - terminal hydrophobic signal sequence . - pubmed
ORF1 was designated the glo gene ( G protein - like ORF ) because its amino acid sequence shows similarity to eukaryotic Gs ( alpha ) protein ( 34.5 % identity over an 81 - amino - acid overlap ) and its C - terminus displays the consensus motif ( CAAX ) which is found in many small eukaryotic GTP - binding proteins . - pubmed
First , the enhancer complex overcomes an inhibitory effect of the sigma 54 leucine patch and unlocks the melting activity of the holoenzyme . - pubmed
The GH receptor ( GHR ) is a protein of 620 amino acids ; the extracellular domain of 246 amino acids is made of two subdomains , one being the domain of interaction with the ligand , the second one being the region of association with another receptor resulting in a homodimer . - pubmed
GH , insulin and sex steroids also influence plasma GHBP levels . - pubmed
This inhibitory action of interferon - alpha in dopamine neurons may be involved in adverse central effects , such as parkinsonism and depression with suicidal potential . - pubmed
Based on this study , Poloxamer 407 was found to be the most effective stabilizer for rhGH for protection against interfacial and thermal stress . - pubmed
Thyroid hormones also decrease the expression of phospholamban , but increase the expression of sarcoplasmic reticulum Ca2 + - pump . - pubmed
As a result , the basal activity of sarcoplasmic reticulum Ca2 + -pump increases , but its beta - adrenergic activation through phosphorylation of phospholamban decreases . - pubmed
Dopamine and the P50 . - pubmed
The swimming training also resulted in an approximately 20 % increase in the insulin - stimulated glucose transport activity ( p & lt ; 0.05 ) of soleus muscle strips and an approximately 3 - fold increase in the plasma membrane GLUT4 protein expression ( p & lt ; 0.01 ) in SHRSP . - pubmed
However , basal and insulin - stimulated glucose transport activity and GLUT4 contents were not significantly different between WKY and SHRSP . - pubmed
Uptake of Tc-99 m sestamibi and Tc-99 m MDP in anaplastic carcinoma of the thyroid ( nondiagnostic CT and ultrasound scans ) . - pubmed
Clavulanic acid restored the ceftazidime activity , thus suggesting an extended spectrum beta - lactamase ( ESBL ) . - pubmed
After cloning and sequencing , the ESBL ( TEM-29B ) showed one amino acid replacement with respect to the TEM-1 sequence , Arg-164 to His . - pubmed
The objectives were 1 ) to investigate the effects of oocyte maturation in serum - free and amino acid - supplemented defined media on oocyte transcript levels , blastocyst cell number , and apoptosis ; 2 ) to investigate the influence of oocyte maturation culture atmosphere on blastocyst development , total cell number , and apoptosis ; and 3 ) to examine the influence of epidermal growth factor ( EGF ) during oocyte maturation on blastocyst cell number and apoptosis . - pubmed
In this study , we used an inhibitor of S - adenosylmethionine decarboxylase , diethylglyoxal bis ( guanylhydrazone)(DEGBG ) , to block the synthesis of spermidine and spermine from putrescine . - pubmed
In addition , the normal distribution of actin filaments required for migration , which is disrupted in polyamine - deficient cells , could be achieved by adding spermine but not putrescine along with DFMO and DEGBG . - pubmed
Since IFN - gamma also stimulates the release of reactive oxygen species ( ROS ) from immunocompetents cells , the amount of neopterin produced also serves as an indirect estimate of oxidative stress . - pubmed
In parallel , IFN - gamma activates the degradation of tryptophan , which appears to limit the growth of intracellular pathogens and the proliferation of cells , including T lymphocytes . - pubmed
VEPs were performed in each case before and after 6 months treatment with either intravenous immunoglobulins ( IVIG ) or steroids . - pubmed
The radiation exposure inside the spacecraft in low earth orbit was investigated with a telescope based on two silicon planar detectors during three NASA shuttle - to - MIR missions ( inclination 51.6 deg , altitude about 380 km ) . - pubmed
Measured mission averaged dose rates in silicon are in the range 98 - 108 microGy d-1 and 137 - 178 microGy d-1 for the GCR and SAA contributions , respectively . - pubmed
The specific aim of this study was to determine the basal cortisol values predicting a normal or impaired response to the insulin hypoglycemia test ( ITT ) . - pubmed
The capability of antibodies to myosin II in abrogating the secretory response from populations of digitonin - permeabilized cells compared with the modest effect caused by anti - myosin V suggests that myosin II plays a fundamental role in the active transport of vesicles occurring in the sub - plasmalemmal area during chromaffin cell secretory activity . - pubmed
The resulting amino acid substitution ( K42N ) in ribosomal protein S12 causes an increased rate of ribosomal proofreading and , as a result , the rate of protein synthesis , bacterial growth and virulence are decreased . - pubmed
Most of the compensatory mutations , resulting in at least 35 different amino acid substitutions , were novel single - nucleotide substitutions in the rpsD , rpsE , rpsL or rplS genes encoding the ribosomal proteins S4 , S5 , S12 and L19 respectively . - pubmed
Effects of erythropoietin and pentoxifylline on the oxidant and antioxidant systems in the experimental short bowel syndrome . - pubmed
In this study , we investigated the effects of erythropoietin ( Epo ) , and pentoxifylline ( Ptx ) on the oxidant and antioxidant systems in the experimental short bowel syndrome . - pubmed
Malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) and glutathione peroxidase ( GSH - Px ) levels were determined in ileum homogenates . - pubmed
When compared to group C , the MDA and GSH - Px levels were significantly decreased ( p & lt ; 0.05 ) , but SOD activity was not changed ( p & gt ; 0.05 ) in groups P and E+P , whereas both MDA and SOD and also GSH - Px activities were not changed significantly in group E ( p & gt ; 0.05 ) . - pubmed
Therefore , our study indicates that Ptx may be more effective than Epo in reducing lipid peroxidation . - pubmed
PI3K - Akt is an important survival signal pathway , that has been shown to be the target of various antitumor drugs , including UCN-01 and geldanamycin , new anticancer drugs under clinical evaluation . - pubmed
Dopamine transporter change in drug - naive schizophrenia : an imaging study with 99mTc - TRODAT-1 . - pubmed
Correlation of specific MPZ mutations with their phenotypes demonstrated that addition of either a charged amino acid or altering a cysteine residue in the extracellular domain caused a severe early onset neuropathy . - pubmed
The effectiveness and efficiency of the tunable incremental factor algorithm is exemplified in the experimental results on clinical indocyanine green angiograms , and the convexity of the SSD function under ideal imaging condition is also illustrated in the paper . - pubmed
Long chain acyl - CoA dehydrogenase ( LCAD ) has recently been shown to be the mitochondrial enzyme responsible for the beta - oxidation of branched chain and unsaturated fatty acids [ Biochim . - pubmed
To identify the generators of the aSSR , 15O - water PET imaging was used to locate the regions of the brain activated by a steady 1 kHz pure tone , the same tone amplitude modulated ( AM ) at 40 Hz and the specific regions of the brain responsive to the AM component of the stimulus relative to the continuous tone . - pubmed
The chemical mechanism by which the enzyme nitrogenase effects the remarkable reduction of N ( 2 ) to NH ( 3 ) under ambient conditions continues to be enigmatic , because no intermediate has been observed directly . - pubmed
Recent experimental investigation of the enzymatic consequences of the valine --> ; alanine modification of residue alpha-70 of the component MoFe protein on the reduction of alkynes , together with EPR and ENDOR spectroscopic characterization of a trappable intermediate in the reduction of propargyl alcohol or propargyl amine ( HCC [ triple bond ] C - CH ( 2 ) OH / NH ( 2 ) ) , has localized the site of binding and reduction of these substrates on the FeMo - cofactor and led to proposed eta ( 2 )- Fe coordination geometry . - pubmed
Here these experimental data are modeled using density functional calculations of the allyl alcohol / amine intermediates and the propargyl alcohol / amine reactants coordinated to the FeMo - cofactor , together with force - field calculations of the interactions of these models with the surrounding MoFe protein . - pubmed
Bradykinin mediates acute inflammation by increasing microvascular permeability , vasodilation , leukocyte migration and accumulation , and the production of arachidonic acid via phospholipase A2 activation . - pubmed
Bradykinin exerts its inflammatory effects via the bradykinin B2 receptor . - pubmed
The aim of this study was to evaluate the effect of a bradykinin B2 receptor antagonist , FR173657 ( FR ) , on intestinal ischemia - reperfusion ( I / R ) injury . - pubmed
The serum thromboxane B2 and 6 - keto - prostagland in F1alpha levels were significantly lower in Group II than in Group I ( P & lt ; 0.05 ) . - pubmed
We conclude that FR treatment appears to have clear protective effects on small bowel I / R injury by inhibiting the release of eicosanoids . - pubmed
BACKGROUND AND AIM : So far , no studies have assessed whether there is an association between iron status and the incidence of major adverse cardiac events or restenosis after coronary stenting . - pubmed
The soluble transferring receptor / ferritin ratio ( sTfR / ferritin ratio ) was used as an index of iron status . - pubmed
The authors assessed 15 - year ( 1985 - 2001 ) incident diabetes ( 148 cases ) , insulin concentration , and insulin resistance ( homeostasis model assessment ) in smokers and nonsmokers according to baseline levels of serum alpha - carotene , beta - carotene , zeaxanthin , beta - cryptoxanthin , and lycopene . - pubmed
It was found that in this model , all formulations of HA and the majority of DPCC lubricants reduced friction ( HA 5 and 10 mg ml(-1 ) , DPPC 200 mg ml(-1 ) reductions of 51.9 % , 46.7 % and 46.5 % respectively ) , compared to a Ringers solution control . - pubmed
By combining HA and DPCC ( HA / DPPC ; 5 mg ml(-1)/100 mg ml(-1 ) and 10 mg ml(-1)/200 mg ml(-1 ) ) , a further improvement was noted ( 69.5 % and 61.9 % , respectively ) as the mean levels of friction were reduced by up to a further 17 % than the most effective individual formulation ( HA 5 mg ml(-1 ) ) . - pubmed
Cyclooxygenase-2 selective NSAIDs such as celecoxib may be less likely to promote gastrointestinal bleeding ; however , there are concerns that they could potentiate the anticoagulation effect of warfarin . - pubmed
Using site - directed mutagenesis , two cysteine residues were introduced in close spatial proximity ( P5C / A135C ) . - pubmed
Chlorophenols ( CPs ) were employed as model compounds to assess the extraction procedure and were determined by gas chromatography - electron - capture detection ( GC - ECD ) . - pubmed
In solid - phase extraction - dispersive liquid - liquid microextraction ( SPE - DLLME ) , CPs were adsorbed from a large volume of aqueous samples ( 100 mL ) into 100 mg functionalized styrene - divinylbenzene polymer ( PPL ) sorbent . - pubmed
This study developed and validated a positive - ion mode liquid chromatography / tandem mass spectrometry ( LC / MS / MS ) method for the quantitative determination of the major ( synephrine ) and minor ( tyramine , N - methyltyramine , octopamine , and hordenine ) adrenergic protoalkaloids in a suite of National Institute of Standards and Technology ( NIST ) bitter orange Standard Reference Materials ( SRMs ): SRM 3258 Bitter Orange Fruit , SRM 3259 Bitter Orange Extract , and SRM 3260 Bitter Orange Solid Oral Dosage Form . - pubmed
LC / MS / MS analysis of the protoalkaloids in the SRMs resulted in mean measurement imprecision levels of & lt ; or = 10 % coefficient of variation ( % CV ) . - pubmed
The PfEMP1(SM ) are encoded by group A var genes that are composed of a more constrained range of amino acid sequences than groups B and C var genes encoding PfEMP1(UM ) associated with uncomplicated malaria . - pubmed
Aldosterone increases oxidant stress to impair guanylyl cyclase activity by cysteinyl thiol oxidation in vascular smooth muscle cells . - pubmed
Soluble guanylyl cyclase ( GC ) is a heterodimer that is activated by NO ( . ) - pubmed
In VSMC treated with aldosterone , Western immunoblotting detected evidence of GC beta(1)-subunit disulfide bonding , whereas mass spectrometry analysis of a homologous peptide containing the Cys-122 - bearing sequence exposed to conditions of increased oxidant stress confirmed cysteinyl sulfinic acid ( m / z 435 ) , sulfonic acid ( m / z 443 ) , and disulfide ( m / z 836 ) bond formation . - pubmed
Exposure to aldosterone or hydrogen peroxide ( H ( 2 ) O ( 2 ) ) significantly decreased cGMP levels in cells expressing wild - type GC . - pubmed
In contrast , aldosterone or H ( 2 ) O ( 2 ) did not influence cGMP levels in cells expressing the mutant C122A GC , confirming that oxidative modification of Cys-122 specifically impairs GC activity . - pubmed
These findings demonstrate that pathophysiologically relevant concentrations of aldosterone increase oxidant stress to convert GC to an NO(. - pubmed
Six 14C - radiolabeled chemicals ranging in lipophilicity were tested , including benzo [ a ] pyrene ( BaP ) , ethylene glycol ( EG ) , methyl parathion ( MP ) , naphthalene ( Nap ) , nonyl phenol ( NP ) , and toluene ( Tol ) . - pubmed
Recently , the blockade of acid - sensing ion channels ( ASICs ) with amiloride and A-316567 attenuated the reflex . - pubmed
Moreover , amiloride had no effect on the mechanoreceptor component of the reflex , prompting us to determine whether ASICs contributed to the metaboreceptor component of the exercise pressor reflex . - pubmed
Also , TCDD group had significantly lower thymic and splenic weights , decreased percentages of CD4(+)CD8(+ ) thymocytes and splenic CD4(+ ) T cells , increased percentage of splenic B220(+)sIgM(+ ) B cells and higher serum interferon gamma concentration . - pubmed
METHODS : We established a quantitative method to determine covalent binding mediated by aldehydes via hemiaminal or hemiacetal using non - radiolabeled compound and [ ( 14 ) C ] semicarbazide as a hard - trap agent with unique post - incubation . - pubmed
RESULTS : The trapped aldehyde obtained from the post - incubation was almost equivalent to the covalent binding of the radiolabeled tool compound . - pubmed
DISCUSSION : The post - incubation method is useful for screening newly synthesized compounds to quantitatively assess the bioactivation of aldehydes descending from alicyclic amines . - pubmed
In addition to a questionnaire , body mass index ( BMI ) , blood pressure , fasting blood glucose , lipids , albumin and serum DHEA - S levels were measured for each participant . - pubmed
Using multivariate logistic regression analyses with adjustments for age , smoking , alcohol , physical activities , albumin and BMI , there was a positive relationship between serum DHEA - S level and metabolic syndrome . - pubmed
Post - translational modification of histone such as acetylation of N - terminal of lysine residues influences gene expression by modulating the accessibility of specific transcription factors to the promoter region , and is essential for a wide variety of cellular processes in the development of individual tissues , including the brain . - pubmed
Systematic examination of protein depositions revealed parenchymal amyloid - beta in 53.8 % , amyloid angiopathy ( Abeta ) in 23.1 % , phospho - tau immunoreactive neuritic profiles in 92.3 % , neurofibrillary degeneration in 38.4 % , new types of tau pathology in 33.3 % , and Lewy - type alpha - synuclein pathology in 15.4 % . - pubmed
These results show that the extracts , fractions , and isolated C - glycosylflavones have an antihyperglycemic action that was reinforced by the stimulation on in vivo insulin secretion . - pubmed
Along with the elevated ASM activity , there also was an elevation of acid ceramidase ( AC ) activity , which was sustained for up to 14 days post - bleomycin treatment . - pubmed
CONCLUSIONS : These data demonstrate that the sphingomyelin / ceramide signaling pathway is involved in the pathogenesis of bleomycin - induced pulmonary fibrosis , and suggest that inhibition of ASM may potentially slow the fibrotic process in the lung . - pubmed
An alternative , one - pot route that avoids use of the highly air - sensitive complex 1 is described for the synthesis of the iron ( II )- lithium complex [ { ( Me ( 3 ) Si )( 2 ) N }( 2 ) Fe { Li ( bta )}]( 2 ) ( 3 ) ( where btaH = benzotriazole ) , in which both iron ( II ) centers reside in 3 - coordinated pyramidal environments . - pubmed
Suspect TBM if there is a CSF leucocytosis ( predominantly lymphocytes ) , the CSF protein is raised , and the CSF plasma glucose is & lt;50 % . - pubmed
As expected , surgery was accompanied by a reduction in serum calcium ( from 2.77 + - 0.2 to 2.25 + - 0.1 mmol / L , p<0.001 ) and parathyroid hormone ( from 29.6 + - 10 to 3.3 + - 2 pmol / L , p<0.001 ) . - pubmed
Inhibitory analysis with TG2 - specific inhibitor KCC-009 suggests that relative contribution of TG2 in total transamidating activity differs in various tissues . - pubmed
The study of PITPs is fundamental to understanding of how the phosphoinositide signal transduction network is regulated and integrated to the larger lipid metabolome in diverse cellular processes . - pubmed
To comprehend how the PITPs integrate phosphoinositide signaling to broader lipid metabolome in diverse cellular processes , it is necessary to devise methods that can correlate the biochemical properties of these non - enzymatic proteins to biologically relevant functional insights . - pubmed
Multimodular polyketide synthases ( PKSs ) have an assembly line architecture in which a set of protein domains , known as a module , participates in one round of polyketide chain elongation and associated chemical modifications , after which the growing chain is translocated to the next PKS module . - pubmed
RecQ5 interacts with Rad51 and is involved in resistance of Drosophila to cisplatin treatment . - pubmed
Moreover , the C - terminal region of the RecQ5 protein and the central region of Rad51 interacted directly and specifically when examined by the glutathione - S - transferase pull - down method . - pubmed
Furthermore , RecQ5 - deficient flies ( recq5 ) were more sensitive to the chemotherapeutic agent cisplatin compared with wild - type ones . - pubmed
Also , Rad51 mutants ( rad51 ) were more sensitive to cisplatin , with sensitivity similar to that of recq5 rad51 double mutants . - pubmed
Sensitive layer - by - layer ( LbL ) coatings for optical detection of gaseous NH ( 3 ) and HCl were prepared by self - assembly of oppositely charged polysaccharides ( chitosan and lambda - carrageenan ) followed by doping LbLs with pH - sensitive dyes - bromothymol blue ( BTB ) and Congo red ( CR ) . - pubmed
CR - doped OWG sensors have shown high sensitivity to HCl vapor but slow relaxation time ( up to several hours for 12 DL LbL films ) . - pubmed
After each 3 - week period , a standardized fat - rich meal was provided , and the effects of liraglutide on triglyceride ( primary endpoint AUC(0 - 8h ) ) , apolipoprotein B48 , non - esterified fatty acids , glycaemic responses and gastric emptying were assessed . - pubmed
FUNDING : Novo Nordisk A / S. RESULTS : After 3 weeks , mean postprandial triglyceride ( AUC(0 - 8h ) liraglutide / placebo treatment - ratio 0.72 , 95 % CI [ 0.62 - 0.83 ] , p = 0.0004 ) and apolipoprotein B48 ( AUC(0 - 8h ) ratio 0.65 [ 0.58 - 0.73 ] , p & lt ; 0.0001 ) significantly decreased with liraglutide 1.8 mg versus placebo , as did iAUC(0 - 8h ) and C(max ) ( p & lt ; 0.001 ) . - pubmed
Mean low - density lipoprotein and total cholesterol decreased significantly with liraglutide versus placebo . - pubmed
X - ray diffraction ( XRD ) and transmission electron microscopic ( TEM ) results establish formation of partially intercalated and partially exfoliated HPMC / MMT nanocomposite films . - pubmed
Using Molecular Mechanics and Molecular Dynamics methods , we investigated at the atomistic level the topography effects both on physisorption on different crystalline planes of TiO2 anatase and on the competitive adsorption when three different crystallographic faces were simultaneously present in an idealized nanosized crystal interacting with a simple heteroaromatic molecule experimentally used in sunlight - induced photosynthetic reaction . - pubmed
Re - irradiation was performed a median of 5.8 years after initial RT , and delivered using lateral opposed beams ( n = 1 ) , IMRT ( n = 4 ) , linear - accelerator based SRS ( n = 3 ) , FSRT ( n = 3 ) , gamma knife surgery ( n = 2 ) , and yttrium-90 brachytherapy ( n = 1 ) . - pubmed
Simulations of EPR spectra have revealed the presence of several types of radicals ( carbon - centred , tyrosyl and semiquinone ) localized in starch and protein fractions of flours . - pubmed
It has been proposed that the canonical 30 nucleotide single - stranded DNA gap generated by nucleotide excision repair is the signal that activates the ATR - mediated DNA damage checkpoint response and that the signal is enhanced by gap enlargement by EXO1 ( exonuclease 1 ) 5 ' to 3 ' exonuclease activity . - pubmed
Here we have used purified core nucleotide excision repair factors ( RPA , XPA , XPC , TFIIH , XPG , and XPF - ERCC1 ) , core DNA damage checkpoint proteins ( ATR - ATRIP , TopBP1 , RPA ) , and DNA damaged by a UV - mimetic agent to analyze the basic steps of DNA damage checkpoint response in a biochemically defined system . - pubmed
[ Design , synthesis and evaluation of bis - nicotine derivatives as inhibitors of cholinesterases and beta - amyloid aggregation ] . - pubmed
The pharmacological results indicated that compounds 3e-3i inhibited both acetylcholinesterase ( AChE ) and butyrylcholinesterase ( BChE ) in the micromolar range ( IC50 , 2.28 - 117.86 micromol x L(-1 ) for AChE and 1.67 - 125 micromol x L(-1 ) for BChE ) , which was at the same potency as rivastigmine . - pubmed
Meth directly stimulated the excitatory node , an inhibitory drive targeted the medullary node , and , in high doses , an additional excitatory drive affected the SPN node . - pubmed
While CtBP1 and CtBP2 share significant amino acid sequence homology , CtBP2 possesses a unique N - terminal domain that is modified by acetylation and contributes to exclusive nuclear localization . - pubmed
The purified RpXDH preferred NAD(+ ) as its coenzyme and showed substrate specificity for xylitol , d - sorbitol , and ribitol . - pubmed
Our results indicate that FRbeta is a promising target for CAR T - cell therapy of AML , which may be augmented by combination with ATRA . - pubmed
Apolipoprotein C - II ( apoC - II ) is the co - factor for lipoprotein lipase ( LPL ) at the surface of triacylglycerol - rich lipoproteins . - pubmed
LPL hydrolyzes triacylglycerol , which increases local surface pressure as surface area decreases and amphipathic products transiently accumulate at the lipoprotein surface . - pubmed
ApoC - II adsorption to a triacylglycerol / water interface resulted in large increases in surface pressure . - pubmed
We characterized apoC - II at phospholipid / triacylglycerol / water interfaces , which more closely mimic lipoprotein surfaces . - pubmed
ApoC - II is provided as a component of triacylglycerol - rich lipoproteins and is the co - factor for LPL as pressure increases . - pubmed
The serum ionic concentration in each group was tested by turbidimetric method ; hematoxylin - eosin staining was used to measure the severity of cochlea hydrops ; immunohistochemical method was used to observe the expression of AQP1 in the cochlea . - pubmed
Combination of UV - B and metals seems more detrimental to the NH4 (+ ) , PO4 ( 3 - ) uptake , GS and APA activity . - pubmed
A significant stimulation in NO3 (- ) uptake and NR activity following exposure to UV - B in all the three cyanobacteria suggests UV - B - induced structural change(s ) in the enzyme / carriers . - pubmed
BACKGROUND : Anoctamin 5 ( ANO5 ) is a member of a conserved gene family ( TMEM16 ) , which codes for proteins predicted to have eight transmembrane domains and putative Ca ( 2 +)-activated chloride channel ( CaCC ) activity . - pubmed
The fo properties were correlated to the average serum levels of bioavailable estradiol ( E2 ) ( mean E2 / sex hormone - binding globulin [ SHBG ] ) and testosterone ( T ) ( mean T / SHBG ) across the second month of life . - pubmed
AIM : The growing implementation of iron oxide nanoparticles in medicine requires a thorough investigation of their physiological influence . - pubmed
These include the alteration of neuronal excitability via specific antigens such as the voltage - gated potassium channel complexes or by mediating bone destruction in rheumatoid arthritis . - pubmed
Mechanisms to explain these cholesterol - related changes are multiple , including alterations in tenocyte gene and protein expression , matrix turnover , tissue vascularity , and cytokine production . - pubmed
BACKGROUND : BAG3 is a 575 amino acid protein that has pleotropic functions in the cell including pro - autophagy and anti - apoptosis . - pubmed
The salutary benefits of rAAV9 - BAG3 were also observed in myocytes isolated from MI hearts including improved cell shortening ( p<0.05 ) , increased systolic [ Ca2+]i , increased [ Ca2+]i transient amplitudes and increased maximal ICa amplitude . - pubmed
decreased activities of anti - oxidant armory ( SOD , CAT , GPx , GR ) and GSH content . - pubmed
MTX significantly causes upregulation of iNOS , Cox-2 , Bax and downregulation of Bcl-2 expressions , it causes higher caspase 3 , 9 activities . - pubmed
CGA inhibited Cox-2 , iNOS , Bax , Bcl-2 and Caspases 3 , 9 mediated inflammation and apoptosis , and improve the histology induced by MTX . - pubmed
The inhibition of thrombin and factor X activities , mediated through antithrombin III and heparin cofactor II of fCSs oligosaccharides were affected by their molecular weight and fucose branches . - pubmed
Oligosaccharides with different sulfation patterns of the fucose branching had a similar ability to inhibit the FXa by the intrinsic factor Xase ( factor IXa - VIIIa complex ) . - pubmed
Furthermore , a heptasaccharide is the minimum size oligosaccharide required for anticoagulation and FXII activation . - pubmed
Molecular size and fucose branch sulfation are important for anticoagulant activity and reduction of size can reverse the activation of FXII caused by native fCSs . - pubmed
CYP2D6 and Endoxifen in Tamoxifen Therapy : A Tribute to David A. Flockhart . - pubmed
Post - Exercise Ingestion of Low or High Molecular Weight Glucose Polymer Solution Does Not Improve Cycle Performance in Female Athletes . - pubmed
The current study sought to evaluate the effects of post - exercise ingestion of a high molecular weight glucose polymer solution ( HMW ) compared to an isocaloric low molecular weight solution ( LMW ) or placebo ( PLA ) on subsequent cycling performance in female athletes . - pubmed
The purpose of this paper is to review studies that examine conditions impacting the degree to which electronic nicotine delivery system ( ENDS ) products substitute for conventional cigarettes in the Experimental Tobacco Marketplace ( ETM ) . - pubmed
Overall , the ETM provides a unique method to explore and identify the conditions by which various nicotine products may interact with one another that mimics the real world . - pubmed
In addition , the ETM permits the efficacy of a broad range of potential nicotine policies and regulations to be measured prior to governmental implementation . - pubmed
Abiraterone could not bind and inhibit the 17 & amp;alpha ;- hydroxylase activity of the CYP17A1 - V366 M mutant . - pubmed
The V366 M mutant also explained the effectiveness of the anti - prostate cancer drug abiraterone as a potent inhibitor of CYP17A1 by binding tightly at the active site in the WT enzyme . - pubmed
The major cellular fatty acid was summed feature 8 ( C18 : 1omega7c and/or C18 : 1omega6c ) , representing 70 % of the total fatty acids . - pubmed
We developed a structure - activity relationship ( SAR ) study of the thiophene molecule 5A. - pubmed
major promastigotes was associated with generation of reactive oxygen species ( ROS ) , and in silico docking studies suggested that 5D may play a role in inhibiting trypanothione reductase . - pubmed
Copper levels affect targeting of hypoxia - inducible factor 1alpha to the promoters of hypoxia - regulated genes . - pubmed
Previous studies have demonstrated that in hypoxia , copper is required for the expression of BNIP3 but not for that of IGF2 Here , using ChIP assays , computational analyses , luciferase reporter assays , and real - time quantitative RT - PCR , we sought to better understand how copper regulates the differential target gene selectivity of HIF-1alpha . - pubmed
The binding of HIF-1alpha to hypoxia - responsive element ( HRE ) sites in the BNIP3 or IGF2 gene promoter in high- or low - copper conditions was examined . - pubmed
Tetraethelenepentamine ( TEPA)-mediated reduction in copper concentration did not affect hypoxia- or CoCl2 - induced HIF-1alpha accumulation . - pubmed
However , the copper reduction did suppress the binding of HIF-1alpha to the HRE ( -412/-404 ) in BNIP3 but not the binding of HIF-1alpha to the HRE ( -354/-347 ) in IGF2 In summary , our findings uncovered the mechanistic basis for differential HIF-1alpha - mediated regulation of BNIP3 and IGF2 , indicating that copper regulates target gene selectivity of HIF-1alpha at least in part by affecting HIF-1alpha binding to its cognate HRE in the promoters of these two genes . - pubmed
Hepatitis B serological test for HBsAg , HBcAb , HBsAb , HBeAg and HbeAb was undertaken using Hepatitis B test kit and capillary blood from the participants . - pubmed
The general prevalence of HBcAb , HBsAg and HBsAb was found to be 3.6 , 2.6 and 19.8 % respectively . - pubmed
BMP2 - loaded titania nanotubes coating with pH - responsive multilayers for bacterial infections inhibition and osteogenic activity improvement . - pubmed