Sentences with gene / chemical entities
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GENE/PROTEIN
CHEMICAL
DISEASE
SPECIES
CELLLINE
Positive correlations were found between the level of
carbonic anhydrase B and arterial
CO2 tension and plasma
HCO3 concentration . -
pubmed
These findings suggest that the synthesis or degradation of
carbonic anhydrase B isozyme is affected by arterial
CO2 tension or plasma
HC03 concentration . -
pubmed
The 4 S to 5 S molar RNA ratio of the monosomes was calculated to be 0.6 , indicating 0.6
tRNA and/or
aminoacyl tRNA molecule per ribosome ; no peptidyl tRNA was present . -
pubmed
Diet , drug therapy (
Sulfonamide derivatives ,
biguanides ,
insulin ) ] . -
pubmed
substance P ( measured as peak increases in lung resistance in cm
H2O / ml per s ) at 6 h following antigen was noted in the presence of
IL-1ra ( 30 mg / kg i.p . ) -
pubmed
The behavioral effects of
DMDS were reversed by
naloxone and the
kappa receptor antagonist
nor - binaltorphimine diHCl , suggesting kappa opioid involvement in these behavioral changes . -
pubmed
Below 14 degrees C , the data are consistent with a model in which interaction of
MgATP with
nitrogenase is fast and irreversible , and is followed by reversible electron transfer . -
pubmed
From the extent and from the rate of the absorbance change , the rate constants for electron transfer from
Fe protein to
MoFe protein and of the reverse reaction were calculated . -
pubmed
Analysis of the data obtained in the temperature range between 6 degrees C and 12 degrees C within the framework of the transition - state theory show that electron transfer from the
Fe protein to the
MoFe protein occurs via a highly disordered transition state with activation parameters delta H(0 ) + + = 289 kJ.mol-1 and delta S(0 ) + + = 792 J.K-1.mol-1 . -
pubmed
Sequence segments of about 140
amino acids in length , each containing a selected consensus region , were used in alignments of the
aminoacyl - tRNA synthetases with the aim of discerning their evolutionary relationships . -
pubmed
Group
II enzymes include those activating
threonine ,
proline ,
serine ,
lysine ,
aspartic acid ,
asparagine ,
histidine ,
alanine ,
glycine , and
phenylalanine . -
pubmed
Within each group ,
enzymes specific for chemically similar
amino acids tend to cluster together , indicating that a major theme of
synthetase evolution involved the adaptation of binding sites to accommodate related
amino acids with subsequent specialization to a single
amino acid . -
pubmed
In a few cases , however ,
synthetases activating dissimilar
amino acids are grouped together . -
pubmed
In order to increase water - solubility , solid complexes of
polyacetylene compounds with
alpha - cyclodextrin (
CD ) were prepared . -
pubmed
Stimulation of platelets with
thrombin leads to rapid degradation of
inositol phospholipids , generation of
diacylglycerol (
DAG ) and subsequent activation of
protein kinase C (
PKC ) . -
pubmed
Previous studies indicated that prior activation of
PKC with
phorbol myristate acetate (
PMA ) desensitizes platelets to
thrombin stimulation , as indicated by a decreased production of
inositol phosphates and decreased
Ca2 + mobilization . -
pubmed
This suggests that
PKC activation generates negative - feedback signals , which limit the
phosphoinositide response . -
pubmed
To test this hypothesis further , we examined the effects of
PKC activators and inhibitors on
thrombin - stimulated
DAG mass formation in platelets . -
pubmed
Pretreatment with
PMA abolishes
thrombin - stimulated
DAG formation ( 50 % inhibition at 60 nM ) . -
pubmed
Pretreatment of platelets with the
PKC inhibitors
K252a or
staurosporine potentiates
DAG production in response to
thrombin ( 3 - 4 - fold ) when using concentrations required to inhibit platelet
PKC ( 1 - 10 microM ) . -
pubmed
K252a does not inhibit phosphorylation of endogenous
DAG or phosphorylation of a cell - permeant
DAG in unstimulated platelets , indicating that
DAG over - production is not due to inhibition of
DAG kinase . -
pubmed
Sphingosine , a
PKC inhibitor with a different mechanism of action , also potentiates
DAG formation in response to
thrombin . -
pubmed
Several lines of evidence indicate that
DAG formation under the conditions employed occurs predominantly by
phosphoinositide ( and not
phosphatidylcholine ) hydrolysis : ( 1 )
PMA alone does not elicit
DAG formation , but inhibits agonist - stimulated
DAG formation ; ( 2 )
thrombin - stimulated
DAG formation is inhibited by
neomycin ( 1 - 10 mM ) but not by the
phosphatidate phosphohydrolase inhibitor
propranolol ; and ( 3 ) no metabolism of
radiolabelled phosphatidylcholine was observed upon stimulation by
thrombin or
PMA . -
pubmed
These data provide strong support for a role of
PKC in limiting the extent of platelet
phosphoinositide hydrolysis . -
pubmed
In 6 a systemic accumulation of
phytanic acid was demonstrated , together with low
phytanic acid oxidase activity in skin fibroblasts in 5 of them . -
pubmed
One dimensional
SDS - PAGE showed four bands , present in control
BLM and ischemic apical membranes , which could not be found in control apical membrane fractions . -
pubmed
The effects of
inositol hexaphosphate (
IHP ) and a second allosteric effector ,
bezafibrate , on the spin - state equilibria of the mixed - spin derivatives of
ferric human hemoglobin A are examined . -
pubmed
ACTH induced significant increases in plasma and salivary
cortisol but in no case was a dose / response relationship detected . -
pubmed
New information about the pharmacokinetics and pharmacodynamics of first - generation
H1 receptor antagonists such as
chlorpheniramine has become available in the past few years . -
pubmed
Comprehensive pharmacokinetic and pharmacodynamic studies of new relatively nonsedating
H1 receptor antagonists such as
terfenadine ,
astemizole ,
loratadine , and
cetirizine are appearing . -
pubmed
Calpain II required 400 microM
calcium , a reducing agent , and pH = 7.5 for maximal activity . -
pubmed
These data suggest that
RU 41740 acts directly on
PMN membrane receptors . -
pubmed
Serum
testosterone and
luteinizing hormone (
LH ) values remained within normal limits indicating resistance of Leydig cells to chemotherapy . -
pubmed
Thus the
drug has markedly altered the subcellular distribution of the
glycoproteins so that they accumulate in the internal smooth membranes but are virtually absent in the plasma membrane . -
pubmed
Significance of elevated urinary
N - acetyl - beta - D - glucosaminidase activity in chronic
cadmium poisoning . -
pubmed
Urinary
NAG activity increased with increasing
beta 2 - m concentration up to about 100 U / g
creatinine ; above this value the NAG activity did not increase with increasing
beta 2 - m concentration . -
pubmed
Urinary
NAG activity thus represents another useful indicator of
renal tubular damage caused by
Cd poisoning since the mechanisms for the excretion of
NAG and
beta 2 - m into urine are thought to be different . -
pubmed
It is concluded that
PCMB releases the blocking effect of intracellular
Na on apical
Na channels . -
pubmed
Bilirubin behaves as a
peroxidase substrate for
prostaglandin H synthase . -
pubmed
Cell sublines of low and high metastatic potential expressed equally abl oncogene - coded mRNA and its phosphoprotein product
p160 , and the capacity of
p160 to become autophosphorylated with
gamma -[ 32P ] ATP was the same among low and high metastatic cells . -
pubmed
Regulation of
corticosteroid hydroxylations : the intramitochondrial dielectric constant and proteolipids of
adrenodoxin and
cytochrome P450 . -
pubmed
Applications : determination of plasma free
fatty acids ,
serum lipase and
post - heparin plasma
triglyceride - lipases ] . -
pubmed
The
heme environment in
horse heart ferrocytochrome c. -
pubmed
Vascular smooth muscle differs from myocardial tissue in several critical ways : ( 1 )
calcium ion entry is achieved by at least two channels ; ( 2 ) both
alpha-1 and alpha-2 postjunctional receptors regulate vascular tone ; ( 3 )
calmodulin increases the activity of the
myosin light chain kinase ; ( 4 )
cyclic AMP decreases rather than increases cytosolic
calcium ion concentration ; ( 5 )
angiotensin increases vascular tone without a positive inotropic effect by acting on
angiotensin receptors which ultimately increase vascular cytosolic
calcium . -
pubmed
About 70 % of
H-2Kb activity could be eluted from
anti - H-2Kb - immunoadsorbents in the presence of 3 M
NH4SCN and
octyl glucoside ( pH 7.4 ) . -
pubmed
On the mechanism of turnover of the
carboxy - terminal
tyrosine of the
alpha chain of
tubulin . -
pubmed
The removal of
tyrosine from the
carboxyl terminus of the
alpha chain of
tubulin occurs predominantly from those
tubulin dimers that are part of microtubules , and is dependent upon microtubular treadmill metabolism . -
pubmed
This factor is neither
tubulin :
tyrosine ligase , the enzyme that catalyzes the addition of
tyrosine to the carboxylterminal position of the
alpha chain of
tubulin , nor a
carboxypeptidase - like activity . -
pubmed
The reactions of
metmyoglobin (
metMb ) and methemoglobin (
metHb ) , oxidized to their respective
oxoferryl free radical species ( .Mb - FeIV = O/.
Hb-4FeIV = O ) by
tert - butyl hydroperoxide (
t - BuOOH ) , with
nitric oxide (
NO . ) -
pubmed
were studied by a combination of optical , electron spin resonance ( ESR ) , ionspray mass (
MS ) , fluorescence , and chemiluminescence spectrometries to gain insight into the mechanism by which
NO . -
pubmed
eliminated the signals of
tyrosyl radical detected by ESR and
oxoferryl detected by
MS in the reaction of
t - BuOOH with
metMb . -
pubmed
As evidenced by
MS of
apomyoglobin , this effect was due to the two - electron reduction of .
Mb - FeIV = O by
NO . -
pubmed
The apparent volume of distribution was directly proportional to the
nonalbumin protein concentration and ranged from 23 to 60 L. We conclude that the pharmacokinetics of
6 - MNA in this population are complicated by possible protein binding changes . -
pubmed
We find that all transfectomas expressing
IgM kappa encoded by
VH 4 - 21 make
IgM autoantibodies reactive with i , a linear
poly - N - acetyllactosamine determinant present on neonatal red blood cells and a
B cell - restricted isoform of the
CD45 surface molecule . -
pubmed
Thrombin induced a sustained contractile response with an initial transient increase in
IP3 to about 160 % of the unstimulated control . -
pubmed
Omission of extracellular
Ca2 + or preincubation with
verapamil ( 10 mumol / L ) reduced the maximum of contraction without significantly affecting the
thrombin - induced increase in
IP3 . -
pubmed
To evaluate the role of
PKC for the contractile response , the
PKC was activated directly by
phorbol 12,13 - dibutyrate (
PDBu , 50 nmol / L ) . -
pubmed
Preincubation with the
PKC inhibitor
staurosporine ( 50 nmol / L ) significantly reduced the
PDBu - induced contraction ( by about 80 % ) . -
pubmed
In
thrombin - stimulated vessels
staurosporine only inhibited the tonic phase of the contractile response whereas the increase in
IP3 and the phasic component of contraction were still evident . -
pubmed
The phasic component of contraction is associated with the generation of
IP3 ; the tonic component might be due to the activation of
PKC . -
pubmed
Microinjection of the excitatory
amino acid L - glutamate into the nucleus tractus solitarii (
NTS ) elicits decreases in arterial pressure and heart rate . -
pubmed
Microinjection of
L - glutamate into the
NTS caused rapid , transient , dose - related decreases in mean arterial pressure and heart rate . -
pubmed
This study suggests that
NTS neurons exert differential effects on renal , mesenteric , and hindquarter vascular beds and that
glutamate - induced regional hemodynamic changes are mediated predominantly through autonomic pathways . -
pubmed
Using altered DNA specificity
Bicoid mutants and appropriate reporter genes , we show that
Bicoid distinguishes among related DNA - binding sites in vivo by a specific contact between
amino acid 9 of its recognition alpha - helix (
lysine 50 of the homeodomain ) and bp 7 of the site . -
pubmed
Our results also demonstrate that
Bicoid binds directly to those genes whose transcription it regulates and that the
amino acid 9 contact is necessary for
Bicoid to direct anterior pattern formation . -
pubmed
In the
beta - lactam resistant strains the enzyme activity and isoelectric points were determined on crude
enzyme extract . -
pubmed
The neurochemistry of
vomiting involves
serotonin and
serotonin S3 receptors . -
pubmed
The major classes of antiemetics include
serotonin S3 receptor antagonists ,
phenothiazines and
metoclopramide . -
pubmed
We usually recommend a
serotonin S3 receptor antagonist alone for less emetogenic regimens or in conjunction with
dexamethasone for more emetogenic regimens . -
pubmed
Subpicogram determination of
oxytocin by an enzyme immunoassay using
acetylcholinesterase as label . -
pubmed
We measured the plasma
ACTH and ME and PVN levels of
CRH , and also of
AVP , because
AVP , another secretagogue of
ACTH , has its cell body in the PVN and axon terminals partly in the ME . -
pubmed
In the control perfusions , the hypothalamic release of
CRH and
AVP and the plasma
ACTH did not change significantly during the entire period of observation. -
pubmed
Ran /
TC4 , in turn , interacts with a number of proteins that are involved in the regulation of
GTP hydrolysis or are components of the nuclear pore . -
pubmed
In solution binding assays ,
Ran -
GTP bound
p97 with high affinity , but the binding of
Ran -
GDP to
p97 was undetectable . -
pubmed
The addition of
RanBP1 with
Ran -
GDP or
Ran -
GTP increased the affinity of both forms of
Ran for
p97 to the same level . -
pubmed
Binding of
Ran -
GTP to
p97 dissociated
p97 from immobilized NLS receptor while the
Ran -
GDP /
RanBP1 /
p97 complex did not dissociate from the receptor . -
pubmed
In a
digitonin - permeabilized cell docking assay ,
RanBP1 stabilizes the receptor complex against temperature - dependent release from the pore . -
pubmed
When added to an import assay with recombinant NLS receptor ,
p97 and
Ran -
GDP ,
RanBP1 significantly stimulates transport . -
pubmed
These results suggest that
RanBP1 promotes both the docking and translocation steps in
nuclear protein import by stabilizing the interaction of
Ran -
GDP with
p97 . -
pubmed
To quantify the relatively large amount of
O2.- generated over a 40 - min period by 1 x 10(6 ) granulocytes / mL , a discontinuous " 10 - min
pulse " method employing
cytochrome c was used ; 140 nmol O2.- per 1 x 10(6 ) cells was detected . -
pubmed
The addition of
catalase to remove extracellular
H2O2 had no effect on DNA breakage . -
pubmed
Renin -
angiotensin system (
RAS ) is a methabolic pathway producing octapeptid
angiotensin II (
AII ) with vasoconstrictive effects , which is also involved in the regulation of water homeostasis and
electrolyte balance in the organism . -
pubmed
The
clock 's responses to light can , however , be reduced when
glucose availability is decreased . -
pubmed
Treatments were 1 ) control ( no added fat ) ; 2 ) tallow ( T ) ,
iodine value (
IV ) = 51.5 ; 3 ) partially hydrogenated tallow ( PHT ) ,
IV = 30.7 ; 4 ) hydrogenated tallow (
HT ) ,
IV = 6.9 ; 5 ) blend ( 1 : 1 ) of
HT and hydrogenated free
fatty acids ( HTHFA ) ,
IV = 9.0 ; and 6 ) hydrogenated free
fatty acids ( HFA ) ,
IV = 11.2 . -
pubmed
Flows of total C16 , total
C18 , and total
fatty acids to the duodenum were increased by supplemental fat ; flows of total
C18 and total
fatty acids were greater than their intake for all treatments . -
pubmed
Digestibility of total
fatty acids entering the small intestine ( 74 , 71 , 62 , 39 , 53 , and 63 % for treatments 1 to 6 , respectively ) was greater for the control diet than for fat - supplemented diets and decreased as either saturation ( T & lt ; PHT & lt ;
HT ) or esterification ( HFA & lt ; HTHFA & lt ;
HT ) increased . -
pubmed
MAIN OUTCOME MEASUREMENTS : Muscle sympathetic nerve activity , assessed by microneurography of the peroneal nerve , blood pressure , heart rate , minute ventilation , transcutaneous
oxygen saturation , and end tidal PCO(2 ) were measured during normal breathing , mask breathing , and
CPAP at 5 and 10 cm
H ( 2 ) O. -
pubmed
Mono - chlorinated harmans were detected by concentration using blue rayon (
BR ) and
GC /
MS analysis in the final effluent from a sewage treatment plant in Shizuoka , Japan . -
pubmed
Six separate
alanine - substituted mutations were generated in 3 highly conserved motifs of
FANCC . -
pubmed
Cells bearing a naturally occurring
FANCC mutation ( 322delG ) that preserves this conserved region showed normal
STAT1 activation but remained hypersensitive to
MMC . -
pubmed
In addition , ( -)
U50,488H stimulation enhanced phosphorylation of the Flag -
hkor , but not Flag -
rkor . -
pubmed
( -)
U50,488H - induced phosphorylation of the Flag -
hkor was reduced by expression of the dominant negative mutant
GRK2 - K220R , demonstrating the involvement of
G protein - coupled receptor kinases (
GRKs ) . -
pubmed
However , expression of
GRK2 and
arrestin-2 or
GRK3 and
arrestin-3 did not result in desensitization or phosphorylation of the Flag -
rkor after ( -)
U50,488H pretreatment . -
pubmed
These results indicate that there are differences in ( -)
U50,488H - induced desensitization and phosphorylation between the
hkor and the
rkor . -
pubmed
Electron paramagnetic resonance (
EPR ) analysis showed two different signals , reflecting the presence of two different types of high - spin
Fe sites with different symmetries . -
pubmed
First , the W71V mutation led to a change in the relative proportion of the two
Fe - based
EPR signals . -
pubmed
LPL plays a dual role in lipid metabolism both in the hydrolysis of
triglyceride - rich lipoproteins and also as a molecular bridge , enhancing the receptor - mediated uptake of lipoproteins both by the liver ( anti - atherogenic ) but also by receptors on the artery wall ( pro - atherogenic ) . -
pubmed
Results from a prospective study on
CAD risk in healthy middle - aged men show that the risk associated with the
LPL - D9N variant , which has a modest effect on plasma
triglyceride levels , is enhanced up to 10 fold , but only in men who smoke . -
pubmed
A
BCR standard reference material of lichen ( CRM 482 ) was used to validate an analytical procedure consisting of a microwave oven digestion associated with ICP - OES , ICP -
MS and GFAAS techniques for the analysis of trace elements (
Al , B ,
Ba ,
Cd ,
Co ,
Cr ,
Cu ,
Fe ,
Mn ,
Ni ,
Pb ,
Ti , V and
Zn ) in biomonitor samples . -
pubmed
All of the SECIS variants were shown to permit
Sec insertion in phage ( by chemical modification of the
Sec residue ) and fused to
lacZalpha ( by
beta - galactosidase assay ) . -
pubmed
Exogenous expression of
PEG10 conferred oncogenic activity and transfection of
hepatoma cells with antisense
S - oligonucleotides suppressing
PEG10 resulted in their growth inhibition . -
pubmed
OBJECTIVE : This study examined strength and bioactive ( remineralizing ) properties of a powder / liquid formulation ( Cement I ) and a more practical two - paste formulation ( Cement
II ) of a
fluoride - releasing resin - based
Ca - PO ( 4 ) cement . -
pubmed
On each tooth , two defined areas were coated , one with a composite resin (
calcium - free control ) , the other with either Cement I or
II . -
pubmed
Corticotropin and
cortisone ; dosage and ratio of intramuscular and intravenous
corticotropin (
ACTH ) and oral
cortisone in treatment of certain
dermatoses . -
pubmed
Some characteristics of coagulation and
von Willebrand factor during short term use of
ticlopidine or
clopidogrel ] . -
pubmed
Levels of F1 + 2 ,
TAT and
vWF were measured at baseline , on days 1 , 3 , 7 and 14 ( 7 days after discontinuation of
thienopyridines ) . -
pubmed
In substudy with
clopidogrel differences between groups existed only in
vWF levels . -
pubmed
This could be interpreted as delayed effect of
ticlopidine on
thrombin activity . -
pubmed
Functional expression of
P2Y1 and
P2Y2 /
P2Y4 , but not
P2Y6 , receptors in intact glomeruli was confirmed by measuring the relative stimulation of the
inositol phosphate pathway induced by selective agonists of a particular receptor subtype . -
pubmed
In the first assay stage , activated
KSR1 is incubated with recombinant
c - Raf-1 and
ATP . -
pubmed
In the second stage , activated
c - Raf-1 is separated from
KSR1 , and incubated with unactivated
MEK1 , unactivated
MAPK ,
Elk-1 , and
ATP . -
pubmed
Further , a complete inactivation of
KSR1 - bound
MEK1 by pretreating with the
MEK inhibitor
PD 98059 prior to the first assay stage did not alter
KSR1 kinase activity . -
pubmed
PURPOSE : Muscle
insulin resistance is characterized by the inability of a normal
insulin concentration to produce a favorable rate of
glucose uptake . -
pubmed
,
glucose transport , is correlated with an increased expression of
GLUT4 protein . -
pubmed
Preventing
GLUT4 overexpression during exercise training will inhibit the improvement in
insulin - stimulated
glucose transport . -
pubmed
Urinary
zinc excretion and serum
insulin were significantly higher in the same group , although the
insulinemia and the parameters of
zinc nutritional status were not significantly correlated . -
pubmed
The
5 - HT4 receptor agonist ,
tegaserod , is a potent
5 - HT2B receptor antagonist in vitro and in vivo . -
pubmed
1
Tegaserod (
Zelnorm ) is a potent
5 - hydroxytryptamine4 ( 5 - HT4 ) receptor agonist with clinical efficacy in disorders associated with reduced gastrointestinal motility and transit . -
pubmed
The present study investigated the interaction of
tegaserod with
5 - HT2 receptors , and compared its potency in this respect to its
5 - HT4 receptor agonist activity . -
pubmed
5 The data from this study indicate that
tegaserod antagonizes
5 - HT2B receptors at concentrations similar to those that activate
5 - HT4 receptors . -
pubmed
It remains to be determined whether this
5 - HT2B receptor antagonist activity of
tegaserod contributes to its clinical profile . -
pubmed
In the first step of protein synthesis ,
aminoacyl - tRNA synthetases react with
amino acid and
ATP to form an enzyme - bound
adenylate that , in the next step , reacts with
tRNA to form
aminoacyl -
tRNA . -
pubmed
The reaction liberates
pyrophosphate (
PP ( i ) ) which , in turn , can be hydrolyzed by
pyrophosphatase to drive efficient aminoacylation . -
pubmed
The bound
adenylate does not react efficiently with the liberated PP(i ) that normally competes with
tRNA to resynthesize
ATP and free
amino acid . -
pubmed
Effects of
histone deacetylase inhibitor
FR901228 on the expression level of
telomerase reverse transcriptase in
oral cancer . -
pubmed
In the present study , the epigenetic effects of the
histone deacetylase (
HDAC ) inhibitor
FR901228 on
hTERT transcription in
oral cancer cell lines were analyzed by RT - PCR . -
pubmed
Moreover , cotreatment of protein synthesis inhibitor
cycloheximide (
CHX ) resulted in the induction of
hTERT transcription by
FR901228 . -
pubmed
This suggests that the induction of
hTERT by
FR901228 requires de novo protein synthesis to some extent and is more likely a direct than an indirect effect on epigenetic changes such as histone acetylation / deacetylation . -
pubmed
We further examined the effect of
FR901228 on
c - myc protein , which is one of the main
hTERT transcription activators . -
pubmed
FR901228 repressed
c - myc protein only in the absence of
CHX , and depended on the enhancement of de novo protein synthesis . -
pubmed
Our results indicate that
c - myc protein is repressed indirectly by
FR901228 but may not contribute to
FR901228 - induced
hTERT transcription . -
pubmed
The present study showed that the
HDAC inhibitor
FR901228 induced the
hTERT gene by a complex mechanism that involved
transcription factors other than
c - myc , in addition to inhibition of histone deacetylation . -
pubmed
A ladder of relative proton affinities (
PA ) for a series of modified
uridines ( e.g. -
pubmed
The studied heterodimers are constituted of a modified
nucleoside and either an
amino acid or a
nucleoside with known
PA value . -
pubmed
These results allowed our
PA ladder of
uridine and
deoxyuridine analogs substituted at the C-5 position in the
uracil ring to be extended . -
pubmed
While these complexes are triply
lithium - bridged dimers in the solid state , a monomer / dimer equilibrium is observed in solution by NMR spectroscopy and ESI FT -
ICR MS . -
pubmed
ESI FT -
ICR mass spectrometry provides detailed insight into the mechanisms with which monomeric
triscatecholate complexes as well as single
catechol ligands exchange in the dimers . -
pubmed
The
5alpha - reductase enzymes types 1 and 2 responsible for converting
testosterone to the more potent
androgen dihydrotestosterone are encoded by the
SRD5A1 and
SRD5A2 genes , respectively . -
pubmed
We performed indirect genetic association studies of
SRD5A1 and
SRD5A2 and the
dihydrotestosterone /
testosterone ratio that reflects the activity of
5alpha - reductase in 57 males with
type 2 diabetes . -
pubmed
We found evidence of significant association between a single nucleotide polymorphism in
SRD5A1 and the
dihydrotestosterone /
testosterone ratio ( median 0.10 , interquartile range 0.08 vs. median 0.06 , interquartile range 0.04 , P = 0.009 ) . -
pubmed
These results suggest that functional genetic variants might exist in or around
SRD5A1 that affect the activity of the
5alpha - reductase enzyme type 1 and influence
androgen levels . -
pubmed
The two families represent the
cupredoxins and multicopper
oxidases , both containing blue
copper - binding domains . -
pubmed
Biochemical analysis showed that lethal mutations of
Asp425 ,
Arg463 ,
Arg511 and
His515 in the
kinase module abolished
polynucleotide kinase activity in vitro . -
pubmed
During PWA assessment no quantitative differences were observed in
PAH between both groups nor after the angiography with
oxygen administration . -
pubmed
A left
T12 -
L1 transforaminal epidural
steroid injection was performed using the " safe triangle " technique ; there was appropriate spread of dye that was visualized and recorded as well as a " washout " image . -
pubmed
Heart rate (
HR ) , gas exchange and respiratory parameters were analyzed throughout the trial , and measured blood
lactate concentration ( [ La(bl ) ] at
rest and immediately after the exercise completion . -
pubmed
Detached plant leaves were treated with CF for 6 , 18 and 30 h , and assayed for changes in
hydrogen peroxide content , total
ascorbate and
glutathione pools and redox ratios ( reduced form to total pool ) , as well as for changes in activities of
ascorbate peroxidase ,
glutathione reductase and
glutathione - S - transferase . -
pubmed
Sequence analysis of cDNA clones and analysis of putative translation products revealed that most encoded
amino acid sequences shared the similar conserved motifs found in the cloned plant
disease resistance genes
RPS2 ,
MLA6 ,
L6 ,
RPM1 , and
Xa1 . -
pubmed
A
valine (
Val ) to
alanine (
Ala ) substitution at
amino acid 16 , occurring in the mitochondrial targeting sequence of the
MnSOD gene , has been associated with an increase in
urolithiasis risk . -
pubmed
The effects of the collimator - detector response , including the penetration and scatter components due to high - energy
123I photons , are modeled using precalculated tables of Monte Carlo simulated point - source response functions obtained from sources in air at various distances from the
face of the collimator . -
pubmed
Non - small cell lung cancer : whole - body MR examination for M - stage assessment -- utility for whole - body diffusion - weighted imaging compared with integrated
FDG PET /
CT . -
pubmed
RESULTS : Area under ROC curve ( A(z ) ) values of whole - body MR imaging with DW imaging ( A(z ) = 0.87 , P = .04 ) and integrated
FDG PET /
CT ( A(z ) = 0.89 , P = .02 ) were significantly larger than that of whole - body DW imaging ( A(z ) = 0.79 ) . -
pubmed
Specificity and accuracy of whole - body MR imaging with ( specificity , P = .02 ; accuracy , P & lt ; .01 ) and that without DW imaging ( specificity , P = .02 ; accuracy , P = .01 ) and integrated
FDG PET /
CT ( specificity , P & lt ; .01 ; accuracy , P & lt ; .01 ) were significantly higher than those of whole - body DW imaging . -
pubmed
The formation of full - length mRNA products was facilitated by including in the reaction mixture 100 mM
sodium acetate , high concentrations of
ribonucleoside triphosphates , and
proteinase K. -
pubmed
We found that the PX domain of
FAD49 not only has affinity for
phosphoinositides , but also binds to its third SH3 domain . -
pubmed
Cation complexing of
crown ethers using fluorescence spectroscopy , part
II . -
pubmed
Application of the
FGF-2 inhibitor
polyanethole sulfonic acid (
PAS ) blocked the downregulation of
osteoglycin and interfered with arteriogenesis . -
pubmed
The thermodynamics of reduction and
His ligand protonation have been determined for a range of loop - contraction variants of the electron transferring type 1
copper protein
azurin (
AZ ) . -
pubmed
For
AZPC , in which the native C - terminal loop containing the
Cys ,
His and
Met ligands has been replaced with the shorter sequence from
plastocyanin ( PC ) and
AZAMI , in which the even shorter
amicyanin ( AMI ) loop has been inserted , the thermodynamics of reduction match those of the
protein whose loop has been introduced which are different to the values for AZ . -
pubmed
The results for
AZAMI and
AZPC are primarily due to protein based enthalpic effects related to the interaction of the metal with permanent
protein dipoles from the loop , and to the decreased loop length which favors
His ligand protonation in the cuprous
proteins . -
pubmed
Diary - documented improvements in urgency ,
urge incontinence , frequency ,
nocturia and nocturnal voids were compared with pre - washout ( on
tolterodine ) and
post - washout ( on no drug ) diary entries . -
pubmed
BACKGROUND AND OBJECTIVES :
Dexmedetomidine is a highly selective
alpha2 - adrenoceptor agonist used in anesthesia for its hypnoanalgesic and cardiovascular effects . -
pubmed
A mechanism of action for such chemoprevention has been provided by the agonism of the
peroxisome proliferator - activated receptor - gamma by
5 - ASA , which unifies its efficacy as an anti - inflammatory and chemopreventive agent . -
pubmed
In the future , even more effective agents based on
5 - ASA are expected , based on more powerful agonism of
peroxisome proliferator - activated receptor - gamma ; 5 - ASA preparations have become ' trendy ' again . -
pubmed
Although
HDL - mediated
cholesterol transport to the liver is well studied ,
cholesterol efflux from hepatocytes back to
HDL is less well understood . -
pubmed
,
HDL3 over
HDL2 ; 2 )
NBD - cholesterol efflux was rapid ( detected in 1 - 2 min ) and resolved into fast [ half time ( t((1/2 ) ) ) = 2.4 min , 6 % of total ] and slow ( t((1/2 ) ) = 26.5 min , 94 % of total ) pools , consistent with protein- and vesicle - mediated
cholesterol transfer , respectively ; 3 )
SCP-2 gene ablation increased efflux of
NBD - cholesterol , as well as
[ ( 3 ) H ] cholesterol , albeit less so due to competition by esterification of
[ ( 3 ) H ] cholesterol , but not
NBD - cholesterol ; and 4 )
SCP-2 gene ablation increased initial rate ( 2.3 - fold ) and size ( 9.7 - fold ) of rapid effluxing
sterol , suggesting an increased contribution of molecular
cholesterol transfer . -
pubmed
Other membrane proteins in
cholesterol efflux [
SRB1 and
ATP - binding cassettes ( ABC ) A-1 ,
ABCG-1 ,
ABCG-5 , and
ABCG-8 ] and several soluble / vesicle - associated proteins facilitating intracellular
cholesterol trafficking (
StARDs , NPCs ,
ORPs ) were not upregulated . -
pubmed
However , loss of
SCP-2 elicited twofold upregulation of
liver fatty acid - binding protein (
L - FABP ) , a protein with lower affinity for
cholesterol but higher cytosolic concentration than
SCP-2 . -
pubmed
Ablation of
SCP-2 and
L - FABP decreased
HDL - mediated NBD -
cholesterol efflux . -
pubmed
These results indicate that
SCP-2 expression plays a significant role in HDL - mediated
cholesterol efflux by regulating the size of rapid vs. slow
cholesterol efflux pools and/or eliciting concomitant upregulation of
L - FABP in cultured primary hepatocytes . -
pubmed
Cangrelor is a reversible , potent , competitive inhibitor of the
ADP P2Y ( 12 ) receptor that is administered intravenously and rapidly achieves near complete inhibition of
ADP - induced
platelet aggregation . -
pubmed
These include behavior ; feeding rate changes ; respiration rate ,
oxygen consumption , and osmoregulation alterations ; nucleic acids , protein , and glycogen synthesis ;
cholinesterase activity inhibition ;
ATPase activity ; and oxidative stress responses . -
pubmed
This article describes the morphological and chemical characterization of stimuli - responsive functionalized
silicon surfaces provided in parallel by atomic force spectroscopy (
AFM ) and Fourier transform infrared spectroscopy ( FT - IR ) enhanced by the single - beam sample reference attenuated total reflection method ( SBSR -
ATR ) . -
pubmed
The stimuli - responsive behavior of the surfaces was obtained by grafting - to in melt
carboxyl - terminated
poly - N - isopropylacryl amides (
PNIPAAM ) with different degree of polymerization (
DP ) on
epoxide - functionalized
silicon substrates . -
pubmed
These two
NF - kappaB factors colocalized with
Tax proteins in dotted cytoplasmic structures targeted by
calreticulin , a multi - process
calcium - buffering chaperone . -
pubmed
The
Pt ( II )-complex has a strong ability to quench the intrinsic fluorescence of
HSA . -
pubmed
The aim of the present study was to describe the effects of beta - blocker
metoprolol monotherapy and combined with
calcium channel inhibitor
felodipin on digital microcirculation in primary
Raynaud ' s syndrome . -
pubmed
Action at a distance :
amino acid substitutions that affect binding of the phosphorylated
CheY response regulator and catalysis of dephosphorylation can be far from the
CheZ phosphatase active site . -
pubmed
Furthermore ,
amino acid substitutions distant from the active site can affect
CheZ catalytic activity and
CheYp binding , perhaps via the propagation of structural or dynamic perturbations through a helical bundle . -
pubmed
RON expression was induced by the
tumor promoter ,
phorbol 12 - myristate 13 - acetate (
PMA ) , in
gastric adenocarcinoma AGS cells . -
pubmed
The factors of
metabolic syndrome (
BMI ,
SBP and
DBP , and blood levels of
glucose ,
cholesterol , and
triglycerides ) , separately and combined , were analysed in quintiles and for transformed z - scores ( mean transformed to 0 and standard deviation to 1 ) . -
pubmed
At week 48 , 83 % of the patients ( 61 %
HBeAg - positive ; 92 %
HBeAg - negative ) achieved a virological response and 82 % ( 78 %
HBeAg - positive ; 83 %
HBeAg - negative ) of those with elevated baseline
alanine aminotransferase showed a biochemical response . -
pubmed
Twenty - six per cent ( 14/54 ) of the
HBeAg - positive patients lost
HBeAg and 22 % ( 12/54 ) achieved seroconversion to
anti - HBe . -
pubmed
Examinations included visual acuity , slit - lamp , fundus , fundus
fluorescein angiography ( FFA ) , large vascular ultrasound , digital subtraction angiography ( DSA ) , erythrocyte sedimentation rate ( ESR ) and
reactive protein C (
CRP ) . -
pubmed
Prostanoids are derived from
arachidonic acid by the sequential actions of
phospholipase A ( 2 ) ,
cyclooxygenase (
COX ) , and specific
prostaglandin ( PG ) synthases . -
pubmed
PGI ( 2 ) acts mainly on the
prostacyclin ( IP ) receptor , but because of receptor homology ,
PGI ( 2 ) analogs such as
iloprost may act on other
prostanoid receptors with variable affinities . -
pubmed
PGI ( 2 )/ IP interaction stimulates
G protein - coupled increase in
cAMP and
protein kinase A , resulting in decreased [
Ca(2+)](i ) , and could also cause inhibition of
Rho kinase , leading to vascular smooth muscle relaxation . -
pubmed
In addition ,
PGI ( 2 ) intracrine signaling may target nuclear
peroxisome proliferator - activated receptors and regulate gene transcription . -
pubmed
These observations have raised interest in the use of
COX inhibitors and
PGI ( 2 ) analogs in the management of pregnancy - associated and neonatal
vascular disorders . -
pubmed
The amount of implanted
( 125 ) I seeds was 12 - 20 seeds / person , with a mean of 16.5 seeds / person , and the matching peripheral dosage (
MPD ) was 80~100Gy . -
pubmed
Hyper - methylated miR-203 dysregulates
ABL1 and contributes to the
nickel - induced
tumorigenesis . -
pubmed
A
calyculin A - sensitive
Ser /
Thr phosphatase activity downregulates
TAp63alpha phosphorylation and
ATM kinase mediates phosphorylation . -
pubmed
Atorvastatin is eliminated by
CYP3A4 which follows carrier - mediated uptake into hepatocytes by
OATP1B1 ,
OATP1B3 , and
OATP2B1 . -
pubmed
Multiple clinical studies demonstrated that
OATP inhibition by
rifampin had a greater impact on
atorvastatin systemic concentration than
itraconazole - mediated
CYP3A4 inhibition . -
pubmed
If it is assumed that the blood and hepatocyte compartments are differentiated by the concentration gradient that is established by
OATPs , and if the rate of uptake into the hepatocyte is rate - determining to the elimination of
atorvastatin from the body , then it is hypothesized that blood concentrations may not necessarily reflect liver concentrations . -
pubmed
A
tetrakis ( dibenzylmethanido ) Eu ( III ) complex as a ultraviolet ( UV ) excited phosphor was synthesized , and incorporated with mesoporous
silica as core - shell (
CS ) , outer - shell ( OS ) and intermediate - shell ( IS ) architectures , using a combination of the self - organization process and the Stober method . -
pubmed
The
CS silica nanoparticle casted UV - LED exhibited the best perfomence with strong intensity and excellent thermal stability . -
pubmed
FokI polymorphism in the
vitamin D receptor gene (
VDR ) and its association with
lumbar spine pathologies in the Italian population : a case - control study . -
pubmed
Alterations in
vitamin D homeostasis , mainly involving its
nuclear receptor (
VDR ) , could have a role in the pathophysiology of the spine . -
pubmed
Regulative
peroxidase activity of DNA - linked
hemin by
graphene oxide for fluorescence DNA sensing . -
pubmed
The inhibition effect of
graphene oxide toward the
peroxidase activity of DNA - linked
hemin was identified and conveniently utilized in the design of a homogenous fluorescence strategy for DNA sensing with high sensitivity . -
pubmed
Specimens of tetragonal
zirconia polycrystal (
TZP ) and
aluminum toughened
zirconia (
ATZ ) were modified with glass solder matrices in two configurations which mainly consisted of
SiO2 ,
Al2O3 ,
K2O , and
Na2O. -
pubmed
Several degradation intermediates of
3,4 - DCA such as
1,2 - dichlorobenzene ,
2 - chloro-1,4 - benzoquinone ,
3,4 - dichlorophenyl isocyanate ,
2 - chlorohydroquinone ,
3,4 - dichloronitrobenzene , and
3,4 - dichlorophenol were identified by gas chromatography mass spectrometry (
GC -
MS ) analysis . -
pubmed
BLASTp analysis and phylogenetic relationship strongly suggested that the
amino acid sequence was a member of
HSP70 family . -
pubmed
Multiple sequence alignment revealed that
SmHSP70 and other known
HSP70 were highly conserved , especially in the regions of
HSP70 family signatures , the bipartite nuclear targeting sequence ,
ATP /
GTP - binding site motif and ' EEVD ' motif . -
pubmed
CONCLUSION : The use of preoperative oral 150 mg
pregabalin 1 h before surgery or
IV 0.5 mg
ketamine with induction of anesthesia can reduce postoperative opioid consumption in
breast cancer surgery without change in sedation or
PONV and with a good safety profile . -
pubmed
LSPR spectra of nanoparticulate gold films , including
Au island films prepared by evaporation on glass and annealing as well as immobilized
Au nanoparticle (
NP ) films , were measured in the transmission and reflection modes . -
pubmed
PURPOSE :
Navitoclax (
ABT-263 ) , a novel , oral
Bcl-2 inhibitor , enhances the antitumor effects of chemotherapy in vitro by lowering the apoptotic threshold . -
pubmed
The processed
DBM can be used alone , together with nanostructured
hydroxyapatite ( nanoHA ) , or dispersed in a physiological carrier or hydrogel . -
pubmed
The authors observed that the presence of
PNIPAAM reduced osteoblast adhesion , while the addition of nanoHA increased osteoblast adhesion , proliferation ,
interleukin-6 (
IL-6 ) production , and reduced
lactate dehydrogenase (
LDH ) production . -
pubmed
Increasing concentrations of
PNIPAAM in combination with nanoHA further increased osteoblast proliferation , and decreased
IL-6 and
LDH production . -
pubmed
Incorporation of
PNIPAAM in
DBM enhanced hMSCs proliferation and
collagen type - I production . -
pubmed
Furthermore , a combination of
PNIPAAM and nanoHA further increased
alkaline phosphatase and
osteocalcin production in hMSCs , independently from the concentration of
PNIPAAM . -
pubmed
This study shows that combinations of
DBM with nanoHA and
PNIPAAM seem to offer a promising route to enhance cell activity and induce osteogenic differentiation . -
pubmed
We further showed that abolishing the intramolecular interaction by a
Leu-449 to
Arg mutation or inducing
ATR - dependent
Chk1 phosphorylation by DNA damage disrupts the closed conformation , leading to an open and activated conformation of
Chk1 . -
pubmed
Bioaccumulation factor (
BAF ) values in earthworms were also higher for PFASs ( 4.06 + -2.23 ) than
PBDEs ( 0.02 + -0.02 ) . -
pubmed
We further analyzed the main biofilm components with
NaIO4 ,
proteinase K , and
DNase . -
pubmed
Quantitative real - time RT - PCR revealed positive correlations between biofilm formation restrained by subinhibitory concentrations of
tigecycline and the expression of biofilm potential - associated genes , especially the
AdeFGH efflux pump gene . -
pubmed
Chemical cross - linking and coimmunoprecipitation experiments revealed that
OATP1B1 may form homo - oligomers , possibly through
disulfide bonds . -
pubmed
When wild - type
OATP1B1 was coexpressed with a loss - of - function mutant W258A , cells showed reduced uptake of prototypic substrate
estrone-3 - sulfate ( ES ) . -
pubmed
Light increases the ion and non -
electrolyte permeability of
rhodopsin - phospholipid vesicles . -
pubmed
A rapid method for the purification of supercoiled PM2 DNA by affinity chromatography on
H1 histone covalently coupled to
agarose . -
pubmed
A simple and rapid method is described for the purification of supercoiled PM2 DNA by affinity chromatography on columns of
H1 histone covalently coupled to
agarose . -
pubmed
Increase in
sialic acids removable by
neuraminidase during the shape change of platelets . -
pubmed
The shape change associated with activation gave rise to an increase in
sialic acids removable by
neuraminidase . -
pubmed
[ Determination of
mercury in surface ,
spring , and ground waters ] . -
pubmed
Screening procedures are included for the following categories :
barbiturates and other sedative - hypnotics , anticonvulsants ,
benzodiazepines , antidepressants ,
phenothiazine and
butyrophenone neuroleptics , central stimulants (
amphetamines ,
cocaine ) ,
hallucinogens (
LSD ,
phencyclidine ,
tetrahydrocannabinol ) ,
opioid ( narcotic ) and other potent analgesics , non - opioid analgesics , antihistamines (
histamine H1 - receptor blockers ) , antiparkinsonian drugs , beta - blockers (
beta - adrenoceptor blockers ) , antiarrhythmics ( class I and
IV ) , diuretics , laxatives and their metabolites . -
pubmed
Treatment with
DHT caused a dose - dependent inhibition of testicular
3 beta - hydroxysteroid dehydrogenase / delta 5 - 4 isomerase (
3 beta - HSD ) 2 h later , and this effect was apparent at the dose of 20 micrograms/100 g
body weight ( P less than 0.01 ) . -
pubmed
The inhibitory effect of
3 beta - HSD was not due to a possible interference of
DHT in the enzyme assay , since various concentrations of the
androgen ( 0.1 - 100 mumol / l ) were ineffective as inhibitors of
3 beta - HSD . -
pubmed
The highest dose of
DHT used in this study ( 200 micrograms/100 g
body weight ) resulted in a rapid ( 1 - 2 h ) and transient ( 4 - 6 h ) inhibition ( approximately 80 % ) of
3 beta - HSD activity . -
pubmed
The results presented suggest that the inhibitory effect of the non - aromatizable
androgen DHT is receptor - mediated and involves the synthesis of a factor(s ) that modulates
3 beta - HSD activity . -
pubmed
Perfusion with both
acetylcholine and
epinephrine ( 10(-5 ) to 10(-3 ) M ) produced concentration - dependent increases in mean
CBF ( maximum increases at 10(-3 ) M of 16 and 9 % , P less than 0.05 ) , whereas only
acetylcholine increased mean SLV ( +56 % at 10(-3 ) M , P less than 0.05 ) . -
pubmed
The influence of
calcium ,
sodium , and the
Na+/
Ca2 + antiport on susceptibility to
cytolysin /
perforin - mediated cytolysis . -
pubmed
In NK cell - mediated cytolysis , the degranulation of the NK cell , and the binding and polymerization of its
effector molecule
cytolysin /
perforin , are
Ca2 + dependent . -
pubmed
To determine if increases in the target cell free intracellular
Ca2 + concentration ( [
Ca2+]i ) are also important for
cytotoxicity , we examined the effects of
Ca2 + substitutes , promotors of
Ca2 + influx ; and inhibitors of
Ca2 + efflux on the cytolysis mediated by purified
cytolysin . -
pubmed
Ca2 + promoted
cytolysin - mediated cytolysis but could also inactivate
cytolysin on preincubation in the absence of target cells . -
pubmed
Ba2 + and
Sr2 + could not alone promote
cytolysin - mediated cytolysis but did enhance
Ca2 (+)-driven cytolysis . -
pubmed
Preincubation with
Ba2 + and
Sr2 + did not inactivate
cytolysin . -
pubmed
One interpretation which these results suggested was that neither
Ba2 + nor
Sr2 + were involved in
cytolysin polymerization and pore formation , but could act intracellularly as
Ca2 + analogs once pore formation had occurred . -
pubmed
The synergistic interaction of
cytolysin and the
Ca2 + ionophore
A23187 further supported a role for increased [
Ca2 +]i in cytolysis . -
pubmed
Inhibitors of
Na+/
Ca2 + exchange , namely low
Na+ medium ,
ouabain , and the
amiloride analogs
2 ' , 4 '- dimethylbenzamil ,
5 -( N-4 - chlorobenzyl )- 2 ' , 4'dimethylbenzamil , and
alpha - phenylbenzamil all markedly enhanced
cytolysin - mediated cytolysis . -
pubmed
This open reading frame contains
amino acid sequences identical to 13 N - terminal
amino acids of
purified factor Y , as well as to a 10 -
amino acid internal sequence ( from a
cyanogen bromide fragment ) as determined by gas - phase microsequencing . -
pubmed
Naloxone caused a significant decrease and captorile -- increase of maximum meanings of HR during exercises ( the running on the treadmill during 3.5 min by the velocity of 30 m / min ) , both compounds did n't exert a considerable effect on BP at
rest and during exercises . -
pubmed
An inhibitory effect of
IL 1 on
insulin secretion is not observed when islets are coincubated with an inhibitor of DNA transcription (
actinomycin D ) . -
pubmed
The inhibitory effect of
IL 1 on
insulin secretion also requires mRNA translation , because three structurally distinct inhibitors of protein synthesis (
cycloheximide ,
anisomycin , and
puromycin ) prevent
IL 1 - induced inhibition of
insulin secretion when added to islets after the 1 - h exposure to
IL 1 . -
pubmed
Two - dimensional gel electrophoresis of islet proteins metabolically labeled with
[ 35S ] methionine demonstrates that
IL 1 augments the expression of a 65 - kD ( pl approximately 6.5 ) protein by greater than 2.5 - fold . -
pubmed
Of all
isoenzymes tested the
human isoenzyme mu is most sensitive to the action of both
ethacrynic acid and its
glutathione conjugate . -
pubmed
Furthermore , the content of the
ANT mRNA in adult islets cultured at a high
glucose concentration was increased compared with islets cultured at a low
glucose concentration . -
pubmed
Finally ,
streptozocin - treated islets , which display an impaired
glucose - sensitive
insulin release after 6 days in culture , also contained less of this mRNA than the control islets . -
pubmed
Beta 1 - selective adrenoceptor antagonists : examples of the
2 -[ 4 -[ 3 -( substituted amino )- 2 - hydroxypropoxy ] phenyl ] imidazole class . -
pubmed
An attempt to develop a highly cardioselective
beta - adrenoceptor antagonist devoid of intrinsic sympathomimetic activity ( ISA ) focused on exploring structure - activity relationships around
( S )-[ p -[ 3 -[[ 2 -( 3,4 - dimethoxyphenyl ) ethyl ] amino ]- 2 - hydroxypropoxy ] phenyl ]- 4 -( 2 - thienyl ) imidazole . -
pubmed
( S )- 4 - Acetyl-2 -[[ 4 -[ 3 -[[ 2 -( 3 , 4 - dimethoxyphenyl ) ethyl ] amino ]- 2 - hydroxypropoxy ] phenyl ] methyl ] imidazole ( S-51 ) was demonstrated to be highly cardioselective ( dose ratio
beta 2 /
beta 1 greater than 9333 ) and devoid of ISA . -
pubmed
This indicates that adequate supply and balance of
amino acids are required both for the increase in polysome - associated
ODC - mRNA activity and for the translational and/or posttranslational mechanism(s ) during the induction of hepatic
ODC activity . -
pubmed
Interaction of ligands in
phosphorylase A as monitored by crosslinking and enzymatic modifications : synergism of
glucose and
caffeine manifested in the exposure of N - terminal segment . -
pubmed
Glycogen ,
caffeine and
glucose dissociate
phosphorylase a tetramer to dimers with half - maximum effect at 0.16 % , 1.1 and 71 mM concentration , respectively , as monitored by crosslinking with
dimethyl suberimidate at 18 degrees C. 2 . -
pubmed
These results indicate that the vasorelaxant effect of
butein depended on the endothelium and was mediated by
endothelium - derived relaxing factor (
EDRF ) . -
pubmed
cAMP - specific
phosphodiesterase ( type IV ) activity was potently inhibited by
butein with an IC50 of 10.4 + /- 0.4 microM. -
pubmed
In contrast ,
phosphodiesterase I , III and V activities were inhibited by
butein above 100 microM. -
pubmed
Adenylate cyclase and
guanylate cyclase activities were unchanged by
butein . -
pubmed
These results suggest that the increase of
cAMP formation elicited by
butein is due to the inhibition of
cAMP - specific phosphodiesterase . -
pubmed
PDI also enhanced the yield of
PelC in
DsbA- cells but only in the presence of exogenous
oxidized glutathione . -
pubmed
Taurocholate feeding decreased ileal
reductase activity 48 % , whereas
tauroursocholate , the
7 beta - hydroxy epimer of taurocholate , had no effect . -
pubmed
Feeding
lovastatin ( inhibitor of
HMG - CoA reductase ) stimulated total ileal
HMG - CoA reductase activity threefold in washed microsomes , which were dissociated from the inhibitor . -
pubmed
The inhibition of intestinal
HMG - CoA reductase activity by
taurocholate occurred without accumulation of mucosal
cholesterol . -
pubmed
Furthermore , the stimulation of total ileal
HMG - CoA reductase activity by
lovastatin treatment was observed without a decrease in mucosal
cholesterol . -
pubmed
In summary , the regulation of ileal
HMG - CoA reductase activity by the intestinal luminal flux of
bile acids is dependent on the orientation of the
hydroxyl groups. -
pubmed
Mutations in the
fur (
ferric uptake regulator ) locus that produce an
Atr - acid - sensitive phenotype also eliminate induction of six transiently induced
ASPs . -
pubmed
so that basal and
LH - stimulated ovarian
steroid secretion could be determined . -
pubmed
The
dopamine transporter (
DAT ) in pre - synaptic membranes and the
vesicular monoamine transporter 2 (
VMAT2 ) in membranes of synaptic vesicles are involved in mediating the acute effects of
amphetamine on
dopamine transmission . -
pubmed
Thus , repeated
amphetamine administration exerts modest and regionally selective effects on
DAT and
VMAT2 mRNA expression in subpopulations of midbrain
dopamine neurons . -
pubmed
Instead , we found that the
GST -
Cdc6 can compete with
distamycin A for binding to the DNA molecule . -
pubmed
As
distamycin A is a specific reagent that binds noncovalently to DNA at ( A + T)-rich tracks , the stimulation of
Abf1 DNA binding activity may be mediated by the
Cdc6 / DNA interaction . -
pubmed
The method involved precipitation of plasma
protein with
methanol followed by analysis of the supernatant using reversed - phase column and UV detection at 316 nm . -
pubmed
The
amino acid transport system y+
L / 4F2hc is a heteromultimeric complex . -
pubmed
We show that expression of system y+L - like
amino acid transport activity by
4F2hc in oocytes is limited by an endogenous factor and that direct covalent modification of external
cysteine residue(s ) of an oocyte membrane protein blocks system y+L/
4F2hc transport activity , based on the following . -
pubmed
Mutation
CS2 had no effect on the expressed system y+L - like transport activity , whereas C109S - containing mutants (
CS1 and
Cys - less ) retained only partial y+L - like transport activity ( 30 to 50 % of wild type ) . -
pubmed
4 ) Sensitivity to
Hg2 + inactivation is increased by pretreatment of oocytes with
ss - mercaptoethanol or in the C109S - containing mutants (
CS1 and
Cys - less ) . -
pubmed
These results indicate that
4F2hc is intimately associated with a membrane oocyte protein for the expression of system y+L
amino acid transport activity . -
pubmed
The ribozyme inhibited the formation of
P - glycoprotein and reduced the cell 's resistance to
adriamycin . -
pubmed
Three
RIIalpha isoforms of 51 , 54 , and 57 kDa were identified by
RIIalpha immunodetection and labeling with
8 - azido [ 32P ] cAMP in different cell cycle phases . -
pubmed
The membrane - bound
quinohaemoprotein alcohol dehydrogenases pass electrons to membrane
ubiquinone which is then oxidized directly by
ubiquinol oxidases . -
pubmed
The electron acceptor for membrane
glucose dehydrogenase is
ubiquinone which is subsequently oxidized directly by
ubiquinol oxidases or by electron transfer chains involving
cytochrome bc1 ,
cytochrome c and
cytochrome c oxidases . -
pubmed
Synthesis of the quinoprotein respiratory systems requires production of
PQQ ,
haem and the
dehydrogenase subunits , transport of these into the periplasm , and incorporation together with divalent cations , into active quinoproteins and quinohaemoproteins . -
pubmed
Analgesic profile of the
nicotinic acetylcholine receptor agonists ,
( + ) - epibatidine and
ABT-594 in models of persistent inflammatory and
neuropathic pain . -
pubmed
Allosteric interactions of
staurosporine and other
indolocarbazoles with
N -[ methyl -( 3 ) H ] scopolamine and
acetylcholine at
muscarinic receptor subtypes : identification of a second allosteric site . -
pubmed
We have studied the interactions of five
indolocarbazoles with
N -[ methyl -( 3 ) H ] scopolamine (
NMS ) and unlabeled
acetylcholine at
M ( 1 )- M ( 4 ) muscarinic receptors , using equilibrium and nonequilibrium radioligand binding studies . -
pubmed
[ ( 3 ) H ] NMS binding was enhanced by
staurosporine ,
KT5720 , and
KT5823 at
M ( 1 ) and M ( 2 ) receptors , and by
K-252a at
M ( 1 ) receptors . -
pubmed
A range of cooperative effects with
acetylcholine was seen , and , at the
M ( 1 ) receptor ,
KT5720 had a log affinity of 6.4 and enhanced
acetylcholine affinity by 40 % . -
pubmed
The compounds inhibited the dissociation of
[ ( 3 ) H ] NMS to different extents across the
receptor subtypes , with the largest effects at
M ( 1 ) receptors . -
pubmed
In equilibrium binding studies the inhibitory potency of
gallamine at
M ( 1 ) receptors was not affected by
KT5720 , indicating that these agents bind to two distinct allosteric sites and have neutral cooperativity with each other . -
pubmed
In contrast ,
gallamine and
staurosporine had a negatively cooperative or competitive interaction at
M ( 1 ) receptors . -
pubmed
Similarly , the potency and relative effectiveness of
KT5720 for inhibiting
[ ( 3 ) H ] NMS dissociation from
M ( 1 ) receptors were not affected by
gallamine or
brucine , but were affected in a complex manner by
staurosporine . -
pubmed
These results demonstrate that there are at least two distinct allosteric sites on the
M ( 1 ) receptor , both of which can support positive cooperativity with
acetylcholine . -
pubmed
Previous research indicated that
allantoic acid degradation is catalysed by different
enzymes in cultivars Maple Arrow and Williams . -
pubmed
The
enzyme found in Williams requires
manganese as a cofactor . -
pubmed
The solubilization of
proteinases with 0.5 %
Triton X-100 substantially improved the yield of the enzymes . -
pubmed
The high
trans alpha - linolenic acid diet significantly increased the plasma
LDL-:
HDL -
cholesterol ratio by 8.1 % ( 95 % CI 1.4 , 15.3 ; and the total
cholesterol :
HDL -
cholesterol ratio by 5.1 % ( 95 % CI 0.4 , 9.9 ; compared with the low - trans diet . -
pubmed
This was largely explained by an increase in
LDL -
cholesterol on the high - trans diet , while no change was observed in the low - trans group ( mean treatment effect of 4.7 % ( 95 % CI -0.8 , 10.5 ; No effects were found on total
cholesterol and
HDL -
cholesterol ,
triacylglycerols ,
apolipoprotein B and A-1 , and
lipoprotein ( a ) concentrations . -
pubmed
In conclusion ,
trans alpha - linolenic acid may increase plasma LDL-:HDL -
cholesterol and total
cholesterol :
HDL -
cholesterol ratios . -
pubmed
Platelet aggregation in response to
ADP ,
collagen , and
PGE1 was assessed , using optical aggregometry . -
pubmed
Addition of
PGE1 prior to addition of an agonist inhibited
platelet aggregation in a concentration - dependent manner , whereas addition of
PGE1 4 minutes after addition of
ADP resulted in partial reversal of aggregation and
fibrinogen binding . -
pubmed
CONCLUSIONS AND CLINICAL RELEVANCE : A high concentration of
sodium citrate in blood samples decreases plasma concentration of ionized
calcium , resulting in reduced
platelet aggregation and
fibrinogen binding . -
pubmed
Platelet aggregation and
fibrinogen binding can be reversed by
PGE1 , which may result in underestimation of platelet activation . -
pubmed
The analytes were extracted with
methanol followed by isocratic chromatography on a micro
C18 reversed - phase column using
acetonitrile - water , 50:50 ( v / v ) , containing 20 mm
acetic acid at 30 microl min(-1 ) . -
pubmed
Polysaccharide recognition by
surfactant protein D : novel interactions of a
C - type lectin with nonterminal
glucosyl residues . -
pubmed
As with the
mannose - binding proteins , we found significant
hydrogen bonding between the
protein and the vicinal , equatorial
OH groups at the 3 and 4 positions on the
sugar ring . -
pubmed
By using various soluble
glucosyl homopolysaccharides as inhibitors of
SP - D carbohydrate binding , we confirmed the interactions predicted by our modeling studies . -
pubmed
Diagnosis and dosimetry of exposure to
sulfur mustard : development of a standard operating procedure for mass spectrometric analysis of
haemoglobin adducts : exploratory research on
albumin and
keratin adducts . -
pubmed
Experiments were carried out to develop a standard operating procedure for analysis of
sulfur mustard adducts to the
N - terminal valine in
haemoglobin and to explore adduct formation with
albumin and
keratin . -
pubmed
Influx of this model permeant by these cells exhibited characteristics of carrier - mediated membrane transport and was only minimally reduced by the addition of 5 micromol / L
folic acid , a concentration of this natural
folate that would have completely inhibited influx by the
folate receptor . -
pubmed
Gene - expression analysis revealed prominent expression of the
folate receptor in some but not all
mesothelioma cell lines , and in only one case was expression of this receptor gene greater than expression of the reduced
folate carrier gene . -
pubmed
CONCLUSION : In view of the ongoing reduced
folate carrier - mediated internalization that occurs in
mesothelioma cells , these results would seem to argue against a role for the
folate receptor in the internalization and cellular pharmacokinetics of
methotrexate and other classic
folate analogs in this
neoplasm . -
pubmed
Furthermore , chronic
ethanol consumption may damage the liver by inhibiting the hepatotrophic and hepatoprotective actions of
TNF alpha and other
cytokines . -
pubmed
It suggested that liver - associated T lymphocytes are involved in the inflammatory process associated with
alcohol related
liver injury through increased
cytokine secretion (
TNF alpha ) . -
pubmed
Parallel solution- and solid - phase synthesis of
spirohydantoin derivatives as
neurokinin-1 receptor ligands . -
pubmed
The combination of the
3,5 - bis ( trifluoromethyl ) phenyl group with a
spirohydantoin motive as a central scaffold was the basis for the design of a combinatorial library targeted towards the
neurokinin-1 receptor . -
pubmed
strain CT16 , purification , and characterization of a
deoxycytidine deaminase extremely thermostable in the presence of
D , L - dithiothreitol . -
pubmed
A
deoxycytidine deaminase that was extremely thermostable in the presence of
dithiothreitol was found in a mesophilic bacterium isolated from soil . -
pubmed
The
enzyme was purified to a homogeneous
protein by treatment at 100 degrees C ,
ammonium sulfate precipitation , and chromatography on
DEAE - Toyopearl ,
hydroxyapatite , and then
Sephacryl S-100 . -
pubmed
After treatment at 50 degrees C for 15 min in the absence of
dithiothreitol ,
enzyme activity was 44 % of the starting activity ; after treatment at 100 degrees C for 2 h in the presence of 50 mm
dithiothreitol , activity was 56 % of the starting activity . -
pubmed
Dithiothreitol greatly stabilized the
enzyme . -
pubmed
The
enzyme molecule was dissociated into two subunits each of 18 kDa subunit when reduced with
mercaptoethanol . -
pubmed
Lipid peroxidation may be initiated either by
lipoxygenases or by reactive
oxygen species ( ROS ) . -
pubmed
PPA1 and
PPB1 display powerful biologic activities including activation of
mitogen - activated protein kinase (
MAPK ) and induction of
glutathione - S - transferase (
GST ) , defense genes , and
phytoalexins . -
pubmed
However , a lack of change in serum
cortisol concentration and body mass and dynamics in
immunoglobulin protein , serum protein , and testes mass were not consistent with previous reports of semelparous dasyurid physiology . -
pubmed
Further experiments indicated that
Ms or Mt
RecA proteins catalyzed
adenosine triphosphate hydrolysis at approximately comparable rates across a wide range of pHs . -
pubmed
These changes were most probably due to specific interactions of the salt ions with the charged
amino acids on the
protein surface rather than due to general effects such as Hofmeister and Debye - Huckel . -
pubmed
We also found significant inverse correlations between OPGc ( corrected for
BMI ) and fasting
glucose ( r = -0.325 , p = 0.005 ) , fasting
insulin ( r = -0.404 , p = 0.0001 ) as well as HOMA - IR ( r = -0.428 , p = 0.0001 ) . -
pubmed
Cerebral MRI revealed diffuse hyperintense T2 abnormalities in the supratentorial white matter without
cerebellar atrophy or lesions , while
Technetium-99m -
ECD brain SPECT revealed a severe
cerebellar hypoperfusion . -
pubmed
Agonists for aggregation were
adenosine diphosphate ,
arachidonic acid ,
collagen ,
epinephrine , and two concentrations of
ristocetin . -
pubmed
Flow cytometry analysis using fluorescence - activated cell sorting with
fluorescein isothiocyanate - labeled
annexin V demonstrated that the
TTSS1 - dependent cell
death was by apoptosis . -
pubmed
AIM : To investigate the distensibility of the common carotid artery ( CCA ) , baroreflex sensitivity ( BRS ) and its relation to plasma
homocysteine concentration in
exfoliation syndrome or
exfoliation glaucoma (
XFS /
XFG ) . -
pubmed
In women independent associations with
ASB were found for
urinary incontinence ( OR : 2.99 , CI : 1.60 - 5.60 ) ,
reduced mobility ( OR : 2.68 , CI : 1.42 - 5.03 ) and
oestrogen treatment ( OR : 2.20 , CI : 1.09 - 4.45 ) . -
pubmed
RASGRF2 expression was restored after treatment with the demethylating agent ,
5 - aza-2 '- deoxycytidine in all four cell lines tested that downregulated
RASGRF2 expression . -
pubmed
Hen egg white lysozyme (
HEWL ) and a synthetic model peptide defining HEWL helix-4 were used as comparative model systems to systematically investigate the effect of increasing
TFE concentrations on the structure of
proteins and peptides . -
pubmed
Higher
TFE concentrations denatured
HEWL with the loss of
lysozyme tertiary structure . -
pubmed
Low Serum
Selenium Is Associated with
Anemia Among Older Women Living in the Community : the Women 's Health and Aging Studies I and
II . -
pubmed
An increase in loge
selenium was associated with a reduced risk of
anemia ( odds ratio per 1 SD increase = 0.63 , 95 % confidence interval = 0.47 - 0.84 ) , adjusting for age , education ,
chronic diseases ,
iron status , and serum
interleukin-6 . -
pubmed
Pattern of
nucleotide substitution and divergence of
prophenoloxidase in decapods . -
pubmed
Despite the unprecedented development in identification and characterization of
prophenoloxidase (
proPO ) in commercially important decapods , little is known about the evolutionary relationship , rate of
amino acid replacement and differential selection pressures operating on
proPO of different species of decapods . -
pubmed
Previously , we showed that
tyrosine phosphorylation of
p130Cas (
Cas ) in a cytoskeletal complex is involved in force - dependent activation of the
small GTPase Rap1 . -
pubmed
In univariate analysis , leukocytes and
CRP levels were associated with
MVO , whereas pre -
infarction angina and prior medication by
aspirin or
calcium channel antagonist appeared protective . -
pubmed
Alteplase (
TPA ) for clotted dialysis catheters . -
pubmed
The patient had received intravenous
dextrose infusions while undergoing a surgical procedure for
post - traumatic
hydrocele elsewhere a week prior to this episode . -
pubmed
Average combined turnover numbers of 35,800 + /- 20,900 ( N = 302 ) and 31,700 + /- 17,700 min(-1 ) ( N = 315 ) were obtained respectively for the in vitro synthesized
enzyme from
strain 35321 with the absence and presence of a C - terminal
His(6 ) tag . -
pubmed
The average combined turnover numbers of the
enzyme from both
strains synthesized in vivo and in vitro and with the presence and absence of a
His(6 ) tag were found to differ significantly . -
pubmed
This indicates that the in vivo and in vitro produced
enzymes are not identical and the presence of a C - terminal
His(6 ) tag alters the activity of
beta - galactosidase . -
pubmed
Separation of the amplification products on 4.5 %
agarose gels allowed the comparative assessment of the classical
type I IFNs . -
pubmed
During follow - up ( postoperative ) , a decrease occurred in the
body mass index ( mean + /- standard deviation 49.2 + /- 8.7 kg / m(2 ) to 34.1 + /- 8.1 kg / m(2 ) ; P & lt;.0001 ) , excess
body weight ( mean + /- SD 76.1 + /- 23.6 kg to 34.9 + /- 21.7 kg ; P & lt;.0001 ) ,
hemoglobin A1c ( mean + /- SD 6.5 % + /- 1.3 % to 5.6 % + /- 0.8 % ; P & lt;.0001 ) ,
systolic blood pressure ( mean + /- SD 133.7 + /- 14.3 mm Hg to 112.9 + /- 14.6 mm Hg ; P & lt ; .0001 ) ,
urine albumin creatinine ratio ( from a median of 8.0 mg / g [ interquartile range 5.0 - 29.3 ] to a median of 6.0 mg / g [ interquartile range 3.3 - 11.5 ] ; P & lt;.0001 ) , and hs -
CRP ( mean + /- SD 11.2 + /- 9.8 mg / L to 4.7 + /- 5.9 mg / L ; P & lt;.0001 ) . -
pubmed
This study was designed to investigate the effects of activation of
cholecystokinin CCK ( 1 ) and
serotonin (
5 - hydroxytryptamine , 5 - HT ) 5 - HT ( 3 ) receptors on pancreatic vagal afferent discharge and to determine whether there is an interaction between these receptors . -
pubmed
The effects of systemic administration of
cholecystokinin and the
serotonin 5 - HT ( 3 ) receptor agonist
phenylbiguanide on pancreatic vagal afferent discharge were recorded before and after administration of
cholecystokinin CCK ( 1 ) and
serotonin 5 - HT ( 3 ) receptor antagonists . -
pubmed
Cholecystokinin CCK ( 1 ) receptor antagonists ,
lorglumide ( 10 mg / kg , i.v . ) -
pubmed
On the other hand ,
serotonin 5 - HT ( 3 ) receptor blockade with
granisetron ( 1 mg / kg , i.v . ) -
pubmed
This study has confirmed that
cholecystokinin and
phenylbiguanide activate pancreatic vagal afferent discharge via activation of
cholecystokinin CCK ( 1 ) and
serotonin 5 - HT ( 3 ) receptors , respectively . -
pubmed
In addition , it has demonstrated that ( i ) the
serotonin 5 - HT(3 ) agonist
phenylbiguanide acts partly via an interaction with
cholecystokinin CCK ( 1 ) receptors , and ( ii ) the actions of
cholecystokinin are not dependent on
serotonin 5 - HT ( 3 ) receptor activation . -
pubmed
Synthesis and antibacterial property of
quinolines with potent
DNA gyrase activity . -
pubmed
BACKGROUND : Studies have suggested that
vitamin D may be important for both
insulin sensitivity and
insulin secretion , and that supplementation with
vitamin D may subsequently prevent development of
type 2 diabetes . -
pubmed
The
insulin and
metformin doses were not changed throughout the study . -
pubmed
RESULTS : After 6 months , the fasting
glucose ,
insulin ,
C - peptide ,
fructosamine , and
HbA ( 1c ) levels were not significantly different from baseline values . -
pubmed
miR-22 overexpression leads to a reduction of
ER alpha levels , at least in part by inducing mRNA degradation , and compromises
estrogen signaling , as exemplified by its inhibitory impact on the
ER alpha - dependent proliferation of
breast cancer cells . -
pubmed
Role of
FOXA in mitochondrial
citrate carrier gene expression and
insulin secretion . -
pubmed
In this study , we have investigated the transcriptional role of the
FOXA site present in the promoter of the mitochondrial
citrate carrier (
CIC ) gene . -
pubmed
podocalyxin like-1 ,
tribbles 3 , reactive
oxygen species , and
nuclear factor kappa B ) ? -
pubmed
Its metal - independent nature , molecular weight of 14.5 kDa , preferential affinity for
beta - D - lactose , and 87 - 92 % identity with
carbohydrate recognition domain of previously reported
galectin-1 confirmed its inclusion in
galectin-1 subfamily . -
pubmed
Treatment of the purified
lectin with oxidizing agent ,
thiol blocking reagents , denaturants , and detergents resulted in significant changes in UV - VIS , fluorescence ,
CD and FTIR spectra , which strongly emphasized the important aspect of regular secondary structure of
galectins for the maintenance of their active conformation . -
pubmed
Reduction of the activity of the purified
lectin after oxidation by
H2O2 , with remarkable fluorescence quenching , may suggest potential role for
galectin-1 in free radical - induced , oxidative stress - mediated
cardiovascular disorders . -
pubmed
PURPOSE : To test interobserver agreement in the
fluorescein /
indocyanine green angiography (
FA / ICGA ) scoring system for
uveitis . -
pubmed
These surrogate factors were fasting
insulin ,
hemoglobin A1c (
HbA1c ) , high - sensitivity
C - reactive protein (
hsCRP ) , and
uric acid . -
pubmed
METHODS : Waist circumference ( WC ) , blood pressure ,
triglycerides ,
high - density lipoprotein cholesterol (
HDL - C ) , fasting
glucose , fasting
insulin ,
HbA1c ,
hsCRP , and
uric acid measurements were obtained from 2,624 adolescent ( 12 - 18 years old ) participants of the 1999 - 2006 National Health and Nutrition Examination Surveys . -
pubmed
Metabolic syndrome criteria had the highest sensitivity for predicting fasting
insulin ( 40 - 70 % ) , followed by
uric acid ( 31 - 54 % ) ,
hsCRP ( 13 - 31 % ) , and HbA1c ( 7 - 21 % ) . -
pubmed
Despite
Wzx homologs performing a conserved function , it has been difficult to pinpoint specific motifs of functional significance in their
amino acid sequences . -
pubmed
Four
TM helices (
II ,
IV , X , and XI ) contain polar residues (
aspartic acid or
lysine ) , and they may form part of a relatively hydrophilic core . -
pubmed
Aspartic acid-85 and
lysine-419 are located in
TM helices
II and XI , while
arginine-298 and
aspartic acid-326 are located in periplasmic and cytosolic loops 4 , respectively . -
pubmed
We propose that the functional requirement of charged residues at both sides of the membrane and in two
TM helices could be important to allow the passage of the
Und - PP - linked
saccharide substrate across the membrane . -
pubmed
FYX-051 : a novel and potent hybrid - type inhibitor of
xanthine oxidoreductase . -
pubmed
4 -[ 5 -( Pyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile (
FYX-051 ) is a potent inhibitor of
bovine milk xanthine oxidoreductase (
XOR ) . -
pubmed
Steady - state kinetics study showed that it initially behaved as a competitive - type inhibitor with a K(i ) value of 5.7 x 10(-9 ) M , then after a few minutes it formed a tight complex with
XOR via a
Mo -
oxygen -
carbon atom covalent linkage , as reported previously ( Proc Natl Acad Sci USA 101:7931 - 7936 , 2004 ) . -
pubmed
The
FYX-051 -
XOR complex decomposed with a half - life of 20.4 h , but the
enzyme activity did not fully recover . -
pubmed
This was found to be caused by
XOR - mediated conversion of
FYX-051 to
4 -[ 5 -( 2 - hydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile (
2 - hydroxy - FYX-051 ) , as well as formation of
6 - hydroxy-4 -[ 5 -( 2 - hydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ] pyridine-2 - carbonitrile (
dihydroxy - FYX-051 ) and
4 -[ 5 -( 2,6 - dihydroxypyridin-4 - yl )- 1H-1,2,4 - triazol-3 - yl ]- 6 - hydroxypyridine-2 - carbonitrile (
trihydroxy - FYX-051 ) during prolonged incubation for up to 72 h. A distinct charge - transfer band was observed concomitantly with the formation of the
trihydroxy - FYX-051 - XOR complex . -
pubmed
Rho - kinase /
nuclear factor - kappabeta /
angiotensinogen axis in
angiotensin II - induced
renal injury . -
pubmed
This work proposes a simple and sensitive solid substrate - room temperature phosphorimetry (
SS -
RTP ) for the selective determination of
carvedilol ( CV ) . -
pubmed
The method is based on the sensitizing effect of
sodium dodecyl benzene sulphonate (
SDBS ) on CV to activate the oxidation between
NaClO and amaranth , resulting in the intense quenching of room temperature phosphorescence (
RTP ) of the system . -
pubmed
Ranolazine ,
nitroglycerin , or saline ( control ) was injected into the left anterior descending (
LAD ) coronary artery or femoral artery ( 2 - mL bolus in 10 seconds ) . -
pubmed
Pretreatment with
prazosin ( 300 mug / kg
IV ) allowed determination of
alpha ( 1 )- adrenergic receptor involvement ( n=8 ) . -
pubmed
Rapid intracoronary administration of
ranolazine ( 0.048 mg / kg ) to achieve high local concentrations resulted in 91 + -11 % increase in
LAD coronary artery flow and 39 + -7 % reduction in coronary vascular resistance ( both , P<0.0001 ) . -
pubmed
Intracoronary
nitroglycerin ( 100 mug ) increased
LAD coronary artery flow by 112 + -25 % ( P=0.02 ) , but the effect lasted & lt;2 minutes ; mean arterial pressure decreased by 4 + -1 mm Hg ( P=0.01 ) . -
pubmed
CONCLUSIONS : Intracoronary or intrafemoral
ranolazine bolus exerts a marked , 2- to 3 - minute dilatory effect that is comparable to
nitroglycerin in magnitude but more persistent , attributable primarily to
alpha ( 1 )- adrenergic blockade . -
pubmed
Tyrosine kinase inhibitors such as
imatinib ,
dasatinib , and
nilotinib interfere with
ATP - binding pocket to inhibit
BCR -
ABL1 kinase . -
pubmed
Treatment with
MMF prevented the motor impairment and significantly reversed the changes in levels of
malondialdehyde ,
glutathione ,
myeloperoxidase and
nitric oxide . -
pubmed
Vasopressin receptor antagonists such as
lixivaptan may provide effective volume unloading . -
pubmed
In the present study ,
Rmi1 was found to be enriched at the region close to an early - firing replication origin when replication forks were arrested near their origins in the presence of
hydroxyurea . -
pubmed
The local synthesis of
type 2 deiodinase (
Dio2 ) promotes
triiodothyronine ( T3 ) production and summer biology , whereas
type 3 deiodinase (
Dio3 ) promotes T3 degradation and winter biology . -
pubmed
Both the central hypointense signal area and the peripheral cogwheel - shaped hypointense signal band in the
tumor were observed on the
post - contrast T1WIs , in accordance with the
necrosis observed in the photomicrographs following
hematoxylin and eosin ( HE ) staining . -
pubmed
Previous studies reported that specific alleles and single
nucleotide polymorphisms ( SNPs ) in the human leukocyte antigen (
HLA ) region were associated with
NOA in East Asians . -
pubmed
CONCLUSION : In unresectable primary
RCC tumors , changes in select
CT parameters after 2 cycles of neoadjuvant
sunitinib might be associated with the potential for surgical resection . -
pubmed
At 15 C , the relationship between
RS ( microl
O ( 2 ) individual(-1 ) h(-1 ) ) and larval dry mass ( M(D ) , microg ) was equal to : R(S ) = 0 0057(+-0 0007 , + - s.e . ) -
pubmed
In mid-2011 , the addition of direct - acting antiviral agents ( DAAs)-the
protease inhibitors
boceprevir (
Victrelis , Merck ) and
telaprevir (
Incivek ,
Vertex)-to
pegylated interferon alpha-2a / b and
ribavirin revolutionized the treatment of
HCV infection by increasing cure rates across all
fibrosis scores in patients with genotype 1
HCV infection . -
pubmed
Overall , the following parameters were found to significantly influence cellulose consumption in a consolidated bioprocess (
CBP ): ( 1 ) the
glucose uptake rate of the culture , ( 2 ) the bacterial cell concentration , and ( 3 ) the nature of the
cellulase enzyme system ( complexed or non - complexed ) . -
pubmed
In 15 % of cases ,
FDG - PET findings are false positive (
FP ) with unclear consequences . -
pubmed
Therefore , we retrospectively investigated the rate of unnecessary procedures due to
FP results on
FDG - PET . -
pubmed
Interestingly ,
USP35 only associates with polarized mitochondria , and rapidly translocates to the cytosol during
CCCP - induced mitophagy . -
pubmed
The current study aims to synthesize and compare the effect of
dexamethasone (
Dex ) release from 2 variants of
Dex - loaded chitosan nanoparticles ( CSnp ) on the odontogenic differentiation of stem cells from apical papilla (
SCAP ) . -
pubmed
Biomineralization of
SCAP was significantly higher ( P & lt ; .05 ) in presence of
Dex - CSnpII compared with that in
Dex - CSnpI at 3 weeks . -
pubmed
Alkaline phosphatase gene expression was significantly higher in the presence of
Dex - CSnpII compared with
Dex - CSnpI , with peak expression seen at 2 weeks ( P & lt ; .05 ) . -
pubmed
The expression of odontogenic specific marker
dentin matrix protein-1 was significantly higher in presence of
Dex - CSnpII compared with
Dex - CSnpI at 3 weeks ( P & lt ; .05 ) . -
pubmed
CONCLUSIONS : Collectively , these data suggest that sustained release of
Dex results in enhanced odontogenic differentiation of
SCAP . -
pubmed
During
ischemia - reperfusion ,
AMPK activation modulates
glucose and
fatty acid metabolism , mitochondrial function , endoplasmic reticulum (
ER ) stress , autophagy , and apoptosis . -
pubmed
Trametinib radiosensitises
RAS- and
BRAF - mutated
melanoma by perturbing cell cycle and inducing senescence . -
pubmed
Thus , we studied whether
trametinib , a potent and selective allosteric inhibitor of
MEK1 / 2 could improve the efficacy of RT . -
pubmed
The effects of
trametinib with and without radiation on protein levels of
MEK effectors were measured by immunoblot analyses . -
pubmed
Trametinib efficiently blocked RT - induced phosphorylation of
ERK at nanomolar concentrations . -
pubmed
A larger population of senescence - activated beta - galactosidase - positive cells was seen in the
trametinib pretreated group , and this correlated with activation of two of the major mediators of induced senescence ,
p53 and
pRb . -
pubmed
CONCLUSION :
Trametinib radiosensitised
RAS-/
RAF - mutated
melanoma cells by inducing prolonged
G1 arrest and premature senescence . -
pubmed
Blood pressure , urinary
albumin /
creatinine ratio , and
serum albumin and
creatinine levels were measured . -
pubmed
There were no significant differences in urinary
albumin /
creatinine ratio as well as
serum albumin and
creatinine levels in Model and Sham groups . -
pubmed
Possible Role of
HER-2 in the Progression of
Prostate Cancer from Primary
Tumor to
Androgen Independence . -
pubmed
The influence of
HER-2 expression on the frequency of biochemical failure and effects of
androgen blockade was determined . -
pubmed
Men CPC ( + ) had a higher frequency of biochemical failure , but there was no difference in
HER-2 expression of CPCs with the frequency of biochemical failure . -
pubmed
After
androgen blockade , men with
HER-2 ( + ) positive secondary
CPCs had a higher frequency of
disease progression to castrate resistant disease . -
pubmed
In the presence of
androgens , there is no survival advantage of expressing
HER-2 , but once biochemical failure has occurred and
androgen blockade started ,
HER-2 positive cells are resistant to treatment , survive and grow leading to castration resistant disease . -
pubmed
BACKGROUND : The
phosphodiesterase-5 inhibitor
sildenafil has been shown to attenuate delayed
cerebral ischemia (
DCI ) and improve neurologic function in experimental
subarachnoid hemorrhage (
SAH ) . -
pubmed
Each underwent
( 15 ) O - PET imaging to measure global and regional
CBF at baseline and
post -
sildenafil . -
pubmed
RESULTS : Mean arterial pressure declined by 10 mm Hg on average
post -
sildenafil ( 8 % , p = 0.01 ) , while ICP was unchanged . -
pubmed
There was no change in global
CBF ( mean 34.5 + - 7 ml/100g / min at baseline vs. 33.9 + - 8.0 ml/100g / min
post -
sildenafil , p = 0.84 ) . -
pubmed
Up - regulating the
abscisic acid inactivation gene
ZmABA8ox1b contributes to seed germination heterosis by promoting cell expansion . -
pubmed
Here we show that increased mutation density at gene promoters can be linked to promoter activity and differential
nucleotide excision repair (
NER ) . -
pubmed
Cost - Effectiveness Analysis of
Ugt1a1 Genotyping Before
Colorectal Cancer Treatment with
Irinotecan . -
pubmed
Specifically , these studies have established that
artemisinin resistance manifests as slow parasite clearance in patients and increased survival of early - ring - stage parasites in vitro ; is caused by single
nucleotide polymorphisms in the parasite 's
K13 gene , is associated with an upregulated " unfolded protein response " pathway that may antagonize the pro - oxidant activity of
artemisinins , and selects for partner drug resistance that rapidly leads to
ACT failures . -
pubmed
Intensifying these efforts should help to forestall the spread of
artemisinin and partner drug resistance from
SEA to sub - Saharan Africa , where the world 's
malaria transmission , morbidity , and mortality rates are highest . -
pubmed
The inhibitor of canonical
NFkappaB activation
parthenolide inhibited the
CCL19 and the early
RANTES responses , but not the
CCL21 or late
RANTES responses . -
pubmed
The gilts were fed one of the following three diets for 14 days : a basic diet for the control group ; the basic diet supplemented with
ZEA - contaminated basic diet for the treatment 1 (
T1 ) group ; and the basic diet supplemented with fermented
ZEA - contaminated basic diet by CK1 for the treatment 2 (
T2 ) group . -
pubmed
The actual
ZEA contents ( analyzed ) were 0 , 1.20 + - 0.11 , 0.47 + - 0.22 mg / kg for the control ,
T1 , and
T2 diets , respectively . -
pubmed
Net
glucose and
insulin production were calculated from portal - arterial differences x portal blood flow , and PDV O consumption was calculated as arterial - portal O differences x portal blood flow . -
pubmed
Indications of chemical bond contrast in
AFM images of a
hydrogen - terminated
silicon surface . -
pubmed
The
lotr1 mutants display altered localization of
CASP1 , an essential protein for Casparian strip formation [ 9 ] , disrupted Casparian strips , ectopic suberization of endodermal cells , and low accumulation of shoot
calcium (
Ca ) . -
pubmed
Ectonucleoside triphosphate diphosphohydrolase 1 (
NTPDase1 ) degrades the
purines ATP and
ADP that are key regulators of
inflammation and clotting . -
pubmed
Prepared nanocomposites were characterized by means of FT - IR ,
1H NMR , Zeta - potential measurements ,
TEM and EDX analyses . -
pubmed
Replication of rs300774 , a genetic biomarker near
ACP1 , associated with suicide attempts in patients with
schizophrenia : Relation to brain
cholesterol biosynthesis . -
pubmed
This SNP also functioned as a trans - eQTL for genes related to
cholesterol biosynthesis and the wnt -
beta - catenin pathway ( p & lt;= 0.0001 ) . -
pubmed
Further , co - expression analysis of candidate genes in brain suggested
ACP1 is important to the regulation of a number of brain mechanisms linked to suicide , including
cholesterol synthesis ,
beta - catenin - mediated signaling pathway ,
serotonin ,
GABA , and the stress response via
ARHGAP35 (
p190rhogap ) , a repressor of
glucocorticoid receptor (
NR3C1 ) transcription . -
pubmed
SA application significantly improved freezing tolerance as evidenced by reduced ion - leakage and alleviated oxidative stress ( lower accumulation of
O2 - and
H2O2 ) following freeze - thaw stress treatments ( -6.5 , -7.5 , and -8.5 C ) . -
pubmed
Improved freezing tolerance of
SA - treated leaves was paralleled by increased
proline and
ascorbic acid (
AsA ) accumulation . -
pubmed
A 9 - d cold acclimation ( CA ) treatment also improved leaf freezing tolerance ( compared to non - acclimated control ) and was accompanied by accumulation of
SA and
proline . -
pubmed
Notably , the beneficial effect of
SA on freezing tolerance was abolished when either
H2O2- or
NO - scavenger ( 1 muM
N - acetylneuraminic acid ,
NANA or 100 muM
hemoglobin , HB , respectively ) was added to
SA as pretreatment . -
pubmed
Glycosaminoglycan (
GAG ) enrichment is a dominant hallmark of early
CAVD , but culture of valvular interstitial cells ( VICs ) in biomaterial environments containing pathological amounts of
hyaluronic acid ( HA ) or
chondroitin sulfate (
CS ) did not directly increase indicators of
disease progression such as VIC activation or inflammatory
cytokine production . -
pubmed
Stretched samples had increased myocardial
cGMP levels , increased phosphorylated vasodilator - stimulated
phosphoprotein phosphorylation , as well as , increased
titin phosphorylation , which was reduced by prior
protein kinase G (
PKG ) inhibition ( PKGi ) . -
pubmed
PT decay after stretching was significantly reduced by combined
NP antagonism ,
NO synthase inhibition and
NO scavenging , or by
PKGi . -
pubmed
However ,
glycated albumin is also affected by
obesity and
albumin , and it is desirable to make a comprehensive judgment with glycated
haemoglobin , random plasma
glucose and other glycaemic index . -
pubmed
Meanwhile , ICS
II repressed the down - regulation of
calbindin protein induced by
IBO . -
pubmed
CONCLUSION : These results suggest that ICS
II attenuates
IBO - induced
cognitive deficits , possibly via the regulation of
calbindin expression and the inhibition of apoptotic response . -
pubmed
In addition , the
MAPK signaling pathway is implicated in the potential mechanisms of ICS
II against
IBO - induced
excitotoxicity , indicating that ICS
II is a promising compound for treatment of
excitotoxicity - related diseases , including
AD . -
pubmed
There are three classes of drugs available for treatment :
endothelin receptor antagonists ( ERA ) , drugs acting on
nitric oxide pathway (
riociguat and
phosphodiesterase type 5 inhibitors [ PDE5i ] ) , and drugs acting on
prostacyclin pathway . -
pubmed
Importantly , a significant modulation of several
psoriasis - related inflammatory
cytokines and
chemokines was observed compared to the high dose of free
EGCG ( p<0.05 ) . -
pubmed
In the bronchoalveolar lavage fluid , GO and
DADS inhibited the elevation in the counts of inflammatory cells , particularly neutrophils , which were induced in the
CS and LPS (
CS + LPS ) group . -
pubmed
Additionally , GO and
DADS inhibited the phosphorylation of
extracellular signal - regulated kinase and the expression of
matrix metalloproteinase-9 in the lung tissues . -
pubmed
Taken together , these findings indicate that GO and
DADS could be a potential preventive agent in
CS - induced
airway inflammation . -
pubmed
Isothiocyanates (
ITCs ) are chemoprotectives produced as the hydrolyzed product of GSLs by enzyme
myrosinase . -
pubmed
Induction of ARE ( antioxidant responsive element ) and ROS ( reactive
oxygen species ) mediated pathway by
Nrf2 controls the activity of
nuclear factor - kappaB (
NF - kappaB ) . -
pubmed
Antagonistic activity of
BITC ,
PEITC and
SFN against
cancer was related with the direct / indirect interaction with
Nrf2 and
NF - kappaB protein . -
pubmed
All three
ITCs able to disrupts
Nrf2 -
Keap1 complex and translocate
Nrf2 into the nucleus . -
pubmed
BITC have the affinity to inhibit the
NF - kappaB than
SFN due to the presence of additional
benzyl structure . -
pubmed
We have also discussed the molecular interaction(s ) of the antagonistic effect of
BITC ,
PEITC and
SFN with
Nrf2 and
NF - kappaB to prevent
cancer . -
pubmed
The hole transport layer - free C -
PSC with a structure of
fluorine - doped
tin oxide / compact
TiO2 / mesoporous
TiO2 /
CH3NH3PbI3 - SWCNT / SWCNT - C gained a remarkable
PCE of 15.73 % with an FF of 0.72 , accompanied by an outstanding stability of 90 days in the dark under high - humidity [ 65 + - 5 % relative humidity (
RH ) , 25 + - 5 C ] and high - temperature ( 75 + - 5 C , 25 + - 5 % RH ) conditions . -
pubmed
rosae was detected for genes from the
phenylpropanoid and
flavonoid pathways and for individual
PR genes , such as paralogs of
PR1 and
PR5 , and other factors of the
salicylic acid signaling pathway . -
pubmed
Sinomenine retards LPS - elicited
inflammation via down - regulating
CCAT1 in HaCaT cells . -
pubmed
Sinomenine pre - administration maintained cell viability , blocked apoptosis and relieved
inflammation with the decrease in
cleaved caspase-3 /- 9 and inflammatory factors . -
pubmed
LPS - induced phosphorylation of
p65 ,
IkappaBalpha and
p38MAPK and overexpression of
CCAT1 were precluded by
sinomenine . -
pubmed
CCAT1 overexpression , which per se induced inflammatory lesions , negated the positive effects of
sinomenine with the restored phosphorylation of
p65 ,
IkappaBalpha , and
p38MAPK . -
pubmed
The anti - inflammatory activity of
sinomenine might be mediated by
CCAT1 down - regulation . -
pubmed
LC -
MS analysis revealed that the extracts contained different classes of
phenolics . -
pubmed
Bone Marrow and
Tumor Radiomics at
18F - FDG PET /
CT : Impact on Outcome Prediction in
Non - Small Cell Lung Cancer . -
pubmed
Each
tumor , its surrounding penumbra , and bone marrow from the
L3 -
L5 vertebral bodies was contoured on pretreatment
FDG PET /
CT images . -
pubmed
At the end of induction treatment , the
CR / PR rate was 43 6/50 9 % and 36 3/60 8 % in the
R - CHOP and R - CVP groups ( P = 0 218 ) respectively . -
pubmed
After a median follow - up of 67 , 66 , and 70 months , five - year
EFS was 61 % vs. 56 % ( not significant ) , progression - free survival ( PFS ) was 71 % vs. 69 % ( not significant ) and overall survival ( OS ) was 84 % vs. 89 % in the R - CHOP vs. the
R - CVP arm respectively . -
pubmed
Butyrate supplementation suppresses
arthritis in a
Breg - dependent manner by increasing the level of the
serotonin - derived metabolite
5 - Hydroxyindole-3 - acetic acid (
5 - HIAA ) , which activates the
aryl - hydrocarbon receptor (
AhR ) , a newly discovered transcriptional marker for Breg function . -
pubmed
Thus ,
butyrate supplementation via
AhR activation controls a molecular program that supports Breg function while inhibiting germinal center (
GC ) B cell and plasmablast differentiation . -
pubmed
One -
pot synthesis of
glycyrrhetic acid polyglycosides based on grafting - from method using
cyclic sulfite . -
pubmed
Glycyrrhetic acid polyglycosides were synthesized in one -
pot via cationic ring - opening condensation polymerization of
cyclic sulfite ( 4 ) initiated by
glycyrrhetic acid as an
aglycon . -
pubmed
To more effectively identify promising
Nrf2 activators through the inhibition of
Keap1 -
Nrf2 PPI , a homogeneous time - resolved fluorescence resonance energy transfer (
TR - FRET ) assay was developed in this work by indirectly labeling the
Keap1 Kelch domain protein with Tb - anti - His antibody as the donor and using , as the acceptor ,
fluorescein isothiocyanate (
FITC)-labeled 9mer
Nrf2 peptide
amide , the same fluorescent probe that was used in an earlier fluorescence polarization (
FP ) assay . -
pubmed
Silencing of
Nrf2 enhanced the degree of apoptosis of EPCs as well as resulted in the impairment of EPC functions through inducing the promotion of ( reactive
oxygen species ) ROS production . -
pubmed
Marein treatment decreased
hypoxia inducible factor-1alpha (
HIF-1alpha ) ,
peroxisome proliferator activated receptor gamma (
PPARgamma ) ,
medium chain acyl - coenzyme A dehydrogenase ,
glucose transporter-4 , and
glycerol-3 - phosphate acyltransferase protein expressions , and increased
PPARalpha ,
fatty acid transport protein-1 ,
carnitine palmitoyltransferase-1 , and
pyruvate dehydrogenase kinase-4 protein expressions . -
pubmed
TRO was faster for the
LAP - ST ( mean = 6.8 min ;
SE = 4.9 ) than standard RUL
ECT ( mean = 15.5 min ;
SE = 6.5 ) . -
pubmed
Our results revealed
NEO and its constituents as potent and efficient
Cx43 GJ inhibitors acting by slow gating mechanism . -
pubmed
GLP-1 regulation by food proteins and
protein hydrolysates . -
pubmed
Glucagon - like peptide 1 (
GLP-1 ) is an enterohormone with a key role in several processes controlling body homeostasis , including
glucose homeostasis and food intake regulation . -
pubmed
We review the literature on the
GLP-1 secretory effects of protein and
protein hydrolysates , and the mechanisms through which they exert these effects . -
pubmed
Crude extracts of the transport - deficient mutant
strain uc-5 pyr-1 were shown to have
uridine 5 '- monophosphate pyrophosphorylase activity comparable to that of the wild - type
strain , suggesting that
uracil uptake in Neurospora does not occur by a group translocation mechanism involving phosphoribosylation . -
pubmed
Specificity studies of
uridine 5 '- monophosphate pyrophosphorylase indicated that phosphoribosylation was not an important determinant of the specificity of
uracil uptake . -
pubmed
Rabbit muscle pyruvate kinase has been shown to catalyze the decarboxylation of
oxalacetate ( Creighton , D.J. -
pubmed
Phosphoenol - alpha - ketobutyrate , an analog of the substrate
phosphoenol - pyruvate , inhibits
decarboxylase and
kinase competitively and with nearly identical Ki values ( 5.7 micron and 4.8 micron , respectively ) . -
pubmed
But , effects of the following modifiers suggest that different
amino acid residues at that site participate in the two reactions :
phenylalanine inhibition and
fructose 1,6 - bisphosphate activation are more effective with the
decarboxylase ;
iodoacetamide preferentially inactivates
decarboxylase while
trinitrobenzenesulfonate preferentially inactivates
kinase . -
pubmed
The purified
enzyme was homogeneous as judged by disc gel electrophoresis and was most active at pH 7.8 using
formaldehyde as a substrate . -
pubmed
The
enzyme was also active toward
acetaldehyde ,
propionaldehyde ,
glyoxal , and
pyruvaldehyde , though the reaction rates were low . -
pubmed
The
enzyme was markedly inhibited by
Ni2 + ,
Pd2 + ,
Hg2 + ,
p - chloromercuribenzoate , and
phenylmethanesulfonyl fluoride . -
pubmed
The molecular weight of the
enzyme was estimated to be 150,000 by the gel filtration method , and analysis by
SDS - polyacrylamide gel electrophoresis indicated that the
enzyme was composed of two subunit monomers . -
pubmed
The
enzyme catalyzed the oxidation of
formaldehyde accompanied by the stoichiometric reduction of
NAD + , but no reverse reaction was observed . -
pubmed
[ Effect of labor on plasma levels of
chorionic somatomammotropin (
CS ) ,
estradiol (
E2 ) ,
progesterone ( Pr ) and
cortisol ; behavior of these levels in the first hours of the puerperium ] . -
pubmed
These studies have shown that the target of this
nalidixic acid effect is dictated by the
recB and
recC genes and may be due to the binding of
nalidixic acid to
exonuclease V. -
pubmed
Growth factors elicit increases in both the
phosphoserine and the
phosphotyrosine content of the
PLC - gamma 1 isozyme . -
pubmed
PLC - gamma 1 contains three
tyrosine phosphorylation sites , which have been identified as residues 771 , 783 and 1254 . -
pubmed
Phosphorylation of
tyrosine residues is sufficient to increase the catalytic activity of
PLC - gamma 1 , though other proteins may modulate this activation . -
pubmed
However , the role of
growth factor - enhanced phosphorylation of
serine residues on
PLC - gamma 1 remains obscure . -
pubmed
In vitro studies of
PLC - gamma 1 , recovered from growth factor - treated cells , indicate that activation by
tyrosine phosphorylation is not due to increased sensitivity to
Ca2 + , a required co - factor , but is reflected in altered kinetic constants , i.e. -
pubmed
Disappearance of a highly unusual clone , 46,XY , del(7)(p12),t(9;22)(q34;q11 ) in
chronic myeloid leukemia after treatment with recombinant
interferon and
cytosine arabinoside . -
pubmed
Conventional chemotherapy with
hydroxyurea and then with recombinant
interferon - alpha (
rIFN - alpha ) did not reduce the population of either subclone . -
pubmed
However , after treatment with a combination of
rIFN - alpha and low - dose
cytosine arabinoside (
LoDac ) continuously infused subcutaneously ( s.c . ) -
pubmed
The Ki values for these
nucleoside inhibitors were similar to their reported Km values as permeants of the
nucleoside transporter . -
pubmed
To test this hypothesis , the endoplasmic reticulum retention sequence
Lys - Asp - Glu - Leu ( KDEL ) was added to the C - terminus of the wildtype
TGF - beta 1 coding sequence , and to a construct in which mutagenesis of two
cysteine residues in the precursor pro region results in the synthesis and secretion of active , as opposed to latent ,
TGF - beta . -
pubmed
These and other observations demonstrate that in a purely " postsynaptic " system , short - term potentiation can be induced and is mediated via
NMDA receptors whereas the potentiated current is carried via non -
NMDA glutamate receptor channels . -
pubmed
The observations of
cell surface antigen expression were paralleled by analysis of
glycolipids isolated from the cells by thin - layer chromatography . -
pubmed
This analysis , in which the new
monoclonal antibodies VINIS-56 and
VIN-2PB-22 were included , also revealed expression of
gangliosides GD3 and
GD2 in all differentiated cultures , albeit at much lower levels following
HMBA exposure than following
retinoic acid or
BUdR - exposure . -
pubmed
The following results were observed : 1 )
vasoactive intestinal polypeptide and
enkephalin did not influence mucociliary activity ; 2 )
bombesin had only a slight accelerating effect on the mucociliary activity at doses of 0.1 to 10 micrograms / kg ; and 3 )
substance P markedly accelerated the mucociliary activity in a dose - dependent manner in the dose range 0.01 to 10 micrograms / kg , the maximal increase being about 50 % . -
pubmed
The effect of
substance P was
atropine - resistant , and probably acted directly on the mucosa . -
pubmed
Cilazapril induced a pronounced increase of
plasma renin activity and
angiotensin I concentrations , whereas after
propranolol both parameters decreased . -
pubmed
After the pharmacological challenges with
angiotensin I and
isoprenaline specific effects of the
ACE inhibitor and
beta - adrenoceptor blocker were found respectively . -
pubmed
At
rest blood pressure was lowered by both drugs , but total peripheral resistance increased after
propranolol and decreased after
cilazapril . -
pubmed
Essentially
fatty - acid - free or essentially
fatty - acid- and
globulin - free
BSA stimulated RTE cell colony formation at low concentrations ( less than 2.5 to 5 mg BSA / ml ) but resulted in concentration - dependent decreases in colony - forming efficiency at higher concentrations . -
pubmed
Finally , commercially available fraction V
BSA prepared by heat
shock , dialysis ,
charcoal treatment , and deionization was stimulatory at low concentrations but inhibitory at high concentrations . -
pubmed
These results suggest that a massive influx of
sodium into the muscle fibers through their cut ends results in maximal stimulation of the
Na +- K +- ATPase of the T - tubular membrane leading to increasing
Na + concentration in the lumen of T - tubules followed by influx of
Cl- to maintain the electrical gradient . -
pubmed
The anterior pituitary gland , in turn , appears to require this episodic
GnRH stimulation in order to secrete the gonadotropins
LH and
FSH in a physiologic fashion . -
pubmed
Replacement regimens of
GnRH which employ an episodic pattern of delivery correct the deficiency of endogenous
GnRH secretion and stimulate
gonadotropin secretion , gonadal steroidogenesis and gametogenesis . -
pubmed
However , continuous occupancy of the
GnRH receptor by native
GnRH and/or its long - acting analogs paradoxically inhibits pituitary
gonadotropin secretion . -
pubmed
GnRH analogs have been synthesized which exhibit greater affinity and prolonged occupancy on the
GnRH receptor and can produce pituitary desensitization of
gonadotropin secretion . -
pubmed
As regards the presence of
carbonic anhydrase in the different types of gastric glandular cells , in addition to the already known function of the enzyme in
hydrochloric acid secretion in the oxyntic and oxyntic - peptic cells , we suggest an interpretation of
carbonic anhydrase presence observed in many chief cells . -
pubmed
In the case of
HDL cholesterol , the differences were 0.23 mmol / l among the men and 0.34 mmol / l among the women , explaining in large part the differences of the mean levels of total
cholesterol . -
pubmed
In serum samples
phospholipase A2 ,
leukotriene C4 ,
prostaglandin E2 and
gamma glutamyl transpeptidase were measured . -
pubmed
These results suggest that
diabetes results in a loss of myocardial sensitivity to
insulin which seems to occur as early as 3 days after induction of
diabetes with
STZ . -
pubmed
Chemical modification of
bovine brain tubulin with the
guanine nucleotide affinity analogue
5 '- p - fluorosulfonylbenzoylguanosine . -
pubmed
Bovine brain tubulin purified in the absence of
GTP and
MgCl2 , reacts with
5 '- p - fluorosulfonylbenzoylguanosine (
5 '- FSBG ) 2 , an affinity analog of
GTP and two moles of the reagent are incorporated per mole of
tubulin at 0 degree C.
5 '- FSBG is unable to promote the polymerization of
tubulin into microtubules . -
pubmed
Tubulin modified with
5 '- FSBG lost two
sulfhydryl groups per mole of
tubulin and reduction with
beta - mercaptoethanol led to the loss of the 2 moles of
FSBG that had been incorporated . -
pubmed
These data are interpreted on the basis that the modification of
tubulin by
5 '- FSBG proceeds via a
thiosulfonate intermediate between the analogue and a reactive
thiol group at or near that portion of the
GTP binding site of
tubulin where the
phosphate moiety of
GTP binds . -
pubmed
Stepwise phosphorolysis with
polynucleotide phosphorylase : a novel method for sequence analysis of
oligoribonucleotides . -
pubmed
[
Trimepranol , a new Czechoslovak sympatholytic
beta receptor blockader . -
pubmed
The effect of
diethyaminoethyl diphenylpropylacetic acid (
SKF 525 - A ) on
uridine 5 '- pyrophosphate glucuronyltransferase . -
pubmed
The
amino acid and nucleotide sequences encoded by the unmutated gene predict that a guanine - to -
adenine transition is responsible for the
glycine - to -
aspartic acid mutation at codon 244 and would also result in the loss of a
HaeIII site . -
pubmed
Substitution of the
hydroxyl group on
C7 of
cholic acid by a
benzamido group leads to a derivative with inhibiting quality for the inward transport of both
bile acids and
phallotoxins by isolated liver cells . -
pubmed
The latter compound was used for affinity labeling of liver plasma membranes in order to detect chemically modified
proteins involved in the transport of
bile acids . -
pubmed
The 67 kDa
protein could be found in the aqueous phase after phase separation in
Triton X-114 . -
pubmed
Aminoacyl - tRNA synthetase fails to activate
benzo ( a ) pyrene-4,5 - dihydrodiol for binding to protein in vitro . -
pubmed
Response to fibrinolytic activity and
factor VIII - related antigen to stimulation with
desmopressin in
hyperlipoproteinemia . -
pubmed
A subnormal activity was invariably found when serum
triglyceride concentration was above 8 mmol / L. The defect can be attributed to low levels of
extrinsic plasminogen activator . -
pubmed
2,2 '- Dipyridyl enhanced the production of chemiluminescence in the presence of
FeSO4 ,
ferritin and
haemosiderin but not
FeCl3 or
horseradish peroxidase . -
pubmed
Nitrilotriacetic acid (
NTA ) enhanced chemiluminescence in the presence of both
FeSO4 and
FeCl3 but not
ferritin or haemosiderin . -
pubmed
GLUT-2 gene transfer into
insulinoma cells confers both low and high affinity
glucose - stimulated
insulin release . -
pubmed
We found that the loss of
glucose sensing in these cells was correlated with the loss of expression of
GLUT-2 and
glucokinase . -
pubmed
GLUT-2 expressing cells also exhibited a larger response to the combination of 5 mM
glucose + 1 microM
forskolin than untransfected cells ( 7.9 versus 1.6 - 2.7 - fold , respectively ) . -
pubmed
Preincubation of
GLUT-2 expressing intermediate passage RIN cells with
2 - deoxyglucose to inhibit low Km
hexokinases resulted in a
glucose - stimulated
insulin secretion response that was shifted toward the physiologic range . -
pubmed
These studies indicate that
GLUT-2 expression confers both a high and low affinity
glucose - stimulated
insulin secretion response to intermediate passage RIN cells . -
pubmed
Verapamil is an antagonist of
L - type Ca2 + channels , and part of its binding site is located in the sixth transmembrane segment (
S6 ) in the fourth repeat of the protein . -
pubmed
Verapamil also blocks
K + channels , which are members of the same supergene family as
Ca2 + channels . -
pubmed
S6 of
fHK has significant homology to that portion of the
verapamil binding site identified in
Ca2 + channels , and
S6 is thought to form part of the inner mouth of K+ channel pores . -
pubmed
Hypertriglyceridemic effect of
fructose is mainly due to an increase in hepatic production of
VLDL . -
pubmed
Cis - acting element named
PKL - III in the 5'-flanking region of this gene is shown to be responsive to dietary
fructose as well as to dietary
glucose . -
pubmed
Overexpression of
calreticulin increases intracellular
Ca2 + storage and decreases store - operated
Ca2 + influx . -
pubmed
The widely distributed and highly conserved
Ca ( 2 +)-binding protein
calreticulin has been suggested to play a role as a
Ca2 + storage protein of intracellular
Ca + stores . -
pubmed
The total cellular
Ca2 + content of
calreticulin - overexpressing and control cells , as assessed by equilibrium
45Ca + 2 uptake , was 141 + /- 8 and 67 + /- 6 pmol of
Ca2+/10(6 ) cells , respectively ( i.e. -
pubmed
a 2.1 + /- 0.2 - fold increase in the
Ca2 + content of
calreticulin - overexpressing cells ) . -
pubmed
In contrast ,
thapsigargin - induced
Ca2 + and
Mn2 + influxes from the extracellular space were markedly diminished in
calreticulin - overexpressing cells , suggesting an active involvement of
calreticulin in the regulation of store - operated
Ca2 + influx . -
pubmed
Rhizobium " hedysari " HCNT1 is an unclassified rhizobium which contains a
nitric oxide - producing
nitrite reductase but is apparently incapable of coupling the reduction of
nitrite to energy conservation . -
pubmed
The gene encoding the
nitrite reductase ,
nirK , has been cloned and sequenced and was found to encode a protein closely related to the
copper - containing family of
nitrite reductases . -
pubmed
Unlike other members of this family ,
nirK expression in
HCNT1 is not dependent on the presence of
nitrogen oxides , being dependent only on
oxygen concentration . -
pubmed
This loss of sensitivity suggests that the product of
nitrite reductase ,
nitric oxide , is responsible for inhibition of
oxygen respiration . -
pubmed
By using a newly developed chemically modified electrode to detect
nitric oxide , it was found that
nitrite reduction by
HCNT1 produces significantly higher
nitric oxide concentrations than are observed in true denitrifiers . -
pubmed
The capacity to generate such large concentrations of freely diffusible
nitric oxide as a consequence of
nitrite respiration makes
HCNT1 unique among bacteria . -
pubmed
A beta production in the TGN persisted even under conditions in which formation of nascent
post - TGN vesicles was inhibited by addition of
guanosine 5 '- O -( 3 - thiotriphosphate ) , a nonhydrolyzable
GTP analogue , or by omission of cytosol . -
pubmed
The kinetic pattern obtained from all groups , shows a significant , five - phased curve : phase I ( 2 - 6 days
post - vaccination ) shows a slow increase in positive
Anthraxin skin reactions . -
pubmed
During phase V ( 91 - 365 days
post - vaccination ) there is a continuous decrease in positive
Anthraxin skin reactions . -
pubmed
The authors studied 50 nondiabetic persons who had completed a euglycemic hyperinsulinemic
clamp protocol in addition to a 75 - g oral
glucose tolerance test and other measures of the coronary risk profile . -
pubmed
Factor 1 consisted of positive loadings for
uric acid , systolic and diastolic blood pressure ,
triglyceride concentration , and waist girth and negative loadings for
HDL cholesterol and the rate of
insulin - mediated
glucose disposal ( M , in milligrams per kilogram of
body weight per minute ) . -
pubmed
M also loaded on factor 2 , along with fasting
insulin and
glucose concentrations ,
diastolic blood pressure , and waist girth . -
pubmed
Placing a number of different peptides at the
COOH termini of a secreted protein and two proteins with type
II membrane anchors resulted in their
TAP - independent presentation . -
pubmed
These findings demonstrate that the secretory compartment possesses
proteases able to liberate
COOH - terminal antigenic peptides from virtually any context , entirely consistent with a role for these
proteases in the processing of
TAP - transported antigenic peptide precursors . -
pubmed
Short - chain fatty acids inhibit intestinal
trefoil factor gene expression in
colon cancer cells . -
pubmed
The
human ITF promoter contained an 11 - residue consensus sequence with high homology to the
butyrate response element of the
cyclin D1 gene . -
pubmed
We conclude that
butyrate inhibits
ITF expression in
colon cancer cells and that this effect may be mediated transcriptionally and independently of its effects on differentiation . -
pubmed
The bispecific ligand - antibody conjugate described in this study is a chemical conjugate between an anti -
CD3 monoclonal antibody (
MAb ) and
folic acid , the ligand for a high - affinity surface receptor expressed on the surface of
choroid plexus tumors . -
pubmed
Phorbol 12 - myristate 13 acetate (
PMA ) , a potent
PKC activator , at 25 nM inhibited the currents through
GABArho2 receptors , which have one consensus phosphorylation site by
PKC in the predicted intracellular loops . -
pubmed
The inhibitory effect of
PMA was also observed after removing each consensus phosphorylation site in both
GABArho1 and
rho2 receptors by site - directed mutagenesis . -
pubmed
New minimal substrate structural requirements in the enzymatic peroxidation of
alkenes with
soybean lipoxygenase . -
pubmed
Substance P - mediated [
Ca2 +]i responses involved generation of
inositol 1,4 , 5 - trisphosphate and release of
Ca2 + from
thapsigargin - sensitive stores , whereas mechanically induced responses were partially ( 29 % ) dependent on
La3 + -sensitive influx of extracellular
Ca2 + and partially on release of intracellular
Ca2 + from
thapsigargin - insensitive stores gated by
ryanodine receptors . -
pubmed
In the later case , the underlying mechanism may involve capacitative
Ca2 + entry channels that are activated after mechanical stimulation but not by
substance P. -
pubmed
SERMs offer
post - menopausal women many of the advantages of
estrogen replacement while mitigating some of the disadvantages , particularly the concern over
breast cancer . -
pubmed
Heterogeneous
long chain acyl - CoA synthetases control distribution of individual
fatty acids in newly - formed
glycerolipids of neuronal cells undergoing neurite outgrowth . -
pubmed
Cells treated with
nerve growth factor (
NGF ) were labeled with
[ 3H ] glycerol ,
[ 3H ] oleic acid ( OA ) or
[ 3H ] arachidonic acid ( AA ) in the presence of other unlabeled
fatty acids of endogenous or exogenous origin . -
pubmed
Triacsin C ( 4.8 microM ) , an inhibitor of
acyl - CoA synthetase , decreased the incorporation of exogenous
[ 3H ] OA into
glycerolipids by 30 - 90 % , and increased by about 60 % the accumulation of free
[ 3H ] OA in the cells . -
pubmed
Activities of the
LCAS that is specific for AA (
ACS ) , or of the non - specific
LCAS which activates OA and other
fatty acids ( OCS ) , were much higher in microsomal and cytoplasmic fractions than in mitochondria or nuclei . -
pubmed
The Vmax and Km values of
ACS and
OCS in microsomes were 12 and 0.7 nmol / min / mg
protein and 70 and 37 microM , respectively ; and in cytoplasm , 6 and 0.6 nmol / min / mg
protein and 38 and 60 microM , respectively . -
pubmed
Triacsin C ( 2 - 33 microM ) did not affect
ACS activity in microsomal or cytoplasmal fractions , but inhibited
OCS activities dose - dependently and competitively : IC50 and apparent Ki values were 13.5 microM and 14 microM in microsomes , and 3.8 microM and 4 microM in cytoplasm . -
pubmed
NGF stimulated the activities of the
LCASs , and , consistently , the incorporation of the various
fatty acids into
glycerolipids . -
pubmed
These data indicate that
LCASs are heterogeneous with respect to their intracellular locations , substrate specificities , kinetic characteristics and sensitivities to
triacsin C ; and that this heterogeneity affects the extents to which individual
fatty acids are utilized to form
glycerolipids . -
pubmed
To clarify whether the
sialyl glycoconjugates work as functional molecules in the process of eosinophil degranulation , we examined the localization of
sialyl residues after degranulation stimulated with
calcium ionophore
A23187 or
interleukin-5 . -
pubmed
Risk assessment based on a clinical score system , myocardial perfusion scintigraphy single photon emission computed tomography ( SPECT ) at
rest and during
adenosine stress , echocardiography , radionuclide angiography , symptom - limited exercise stress test , and 24 - h Holter ECG recording with ST - analysis and analysis of heart rate variability ( HRV ) were performed 5 - 8 days after hospital admission . -
pubmed
Analysis of
tyrosine phosphorylation - dependent protein - protein interactions in
TrkB - mediated intracellular signaling using modified yeast two - hybrid system . -
pubmed
Activated
receptor tyrosine kinases induce a large number of
tyrosine phosphorylation - dependent protein - protein interactions through which they mediate their various ligand - exerted functions including regulation of proliferation , differentiation and survival . -
pubmed
TrkB receptor tyrosine kinase activated by binding of
brain - derived neurotrophic factor (
BDNF ) also stimulates various protein interactions in a
tyrosine phosphorylation - dependent manner in neuronal cells . -
pubmed
To examine
tyrosine phosphorylation - dependent interactions stimulated by active
TrkB , we developed a modified yeast two - hybrid system , which we call the yeast two - and - a - half - hybrid system . -
pubmed
Using this system with
Shp2 as the bait , we demonstrated that
Shp2 interacts directly with
BIT /
SHPS-1 ( also called
SIRP ) and
Grb2 depending on
tyrosine phosphorylation mediated by
TrkB. -
pubmed
We found that
fibroblast growth factor receptor substrate 2beta (
FRS2beta ) , also called
SNT2 , interacts with
Shp2 dependently on
TrkB - mediated
tyrosine phosphorylation of
FRS2beta /
SNT2 . -
pubmed
Therefore , we show that the two - and - a - half - hybrid system is a powerful tool for studying
tyrosine phosphorylation - dependent protein - protein interactions in intracellular signaling pathways stimulated by
TrkB receptor tyrosine kinase . -
pubmed
Hormones exhibit a wide range of biological activities on the skin , with major effects caused by growth hormone /
insulin - like growth factor-1 , neuropeptides , sex
steroids ,
glucocorticoids ,
retinoids ,
vitamin D ,
peroxisome proliferator - activated receptor ligands , and
eicosanoids . -
pubmed
Inhibition of
actin polymerization with
cytochalasin A resulted in similar phenotypes , suggesting that
Scar - mediated polymerization of the
actin cytoskeleton was important in the regulation of these processes . -
pubmed
Possible involvement of
endothelin-1 and
nitric oxide in the pathogenesis of proliferative
diabetic retinopathy . -
pubmed
Endothelin-1 and
NO metabolites were measured by radioimmunoassay and high - performance liquid chromatography based on the Griess method , respectively . -
pubmed
CONCLUSION : These results indicate that
ET-1 and
NO may be related in the pathogenesis of
PDR . -
pubmed
Cellular effects of
purvalanol A : a specific inhibitor of
cyclin - dependent kinase activities . -
pubmed
In synchronized cells ,
purvalanol A induced a reversible arrest the progression in
G1 and
G2 phase of the cell cycle , but did not prevent the completion of DNA synthesis in S - phase cells . -
pubmed
The cell contents of
cyclins D1 and E were lower in cells incubated with
purvalanol A compared to controls , but the level of the
cdk inhibitory protein
p21(
WAF1 /
CIP1 ) was increased , indicating that the drug did not cause a general inhibition of gene expression . -
pubmed
This compound , however , caused an inhibition of the
estradiol - induced expression of an integrated
luciferase gene , suggesting that
cdk or related enzymes may participate in the regulation of the activity of certain promoters . -
pubmed
We found that
Tax - mediated apoptosis in response to UV irradiation was inhibited by
Bcl-2 and
Bcl - X ( L ) overexpression and by treatment with the
caspase inhibitor
z - VAd - FMK . -
pubmed
The phosphorylation of
CREB ,
ATF1 and
MAPKAP - K2 were inhibited by
SB203580 , a
p38MAPK inhibitor , but not by
PD98059 , an
ERK kinase inhibitor . -
pubmed
OBJECTIVES : To compare the in vitro scleral permeability of
carboplatin using either a
fibrin sealant or a
balanced salt solution ( BSS ) vehicle and to measure in vivo ocular tissue levels following subconjunctival injection of
carboplatin in
fibrin sealant or BSS . -
pubmed
RESULTS : In vitro
carboplatin in
fibrin sealant had a peak permeability constant of 13.7 + /- 2.3 x 10(-6 ) cm / s ;
carboplatin in BSS , 27.0 + /- 1.7 x 10(-6 ) cm / s. After 24 hours , 33.2 % + /- 1.8 % of the
carboplatin was retained in the
fibrin sealant , while 5.5 % + /- 1.0 % was retained in the BSS . -
pubmed
In vivo subconjunctival injection of
carboplatin in
fibrin sealant vehicle achieved 11.83 + /- 5.16 microg / mL in the vitreous at 1(1/2 ) hours and 0.03 + /- 0.06 microg / mL in the vitreous at 2 weeks . -
pubmed
CONCLUSION :
Fibrin sealant provided a more controlled and localized release of
carboplatin and delivered
carboplatin to the ocular tissues for up to 2 weeks . -
pubmed
CLINICAL RELEVANCE : This study reports the use of
fibrin sealant as a subconjunctival delivery vehicle for
carboplatin , and quantifies ocular drug levels achieved in an animal model . -
pubmed
The purpose of the present study was to investigate the effects of an
ECE inhibitor ,
CGS 26303 , on hemolysate - induced
injury of cerebral vessel endothelial cells as well as the production of
ET-1 from these cells . -
pubmed
Treatment with
CGS 26303 inhibited the hemolysate - induced increases in the levels of
ET-1 and big
ET-1 and reduced endothelial cell
injury . -
pubmed
During
MPTP administration , phosphorylation of
p - Jun was increased in the substantia nigra , and
MKK4 was increased both in the striatum and substantia nigra . -
pubmed
Nucleotide - based amplification method is an important system for the identification of genomic modified foods (
GMF ) . -
pubmed
In the
potassium channel KcsA and in the cation channel
gramicidin , permeant selectivity and mobility are determined by the proteinaceous matrix via
hydrogen bonding , charge - dipole , and dipole - dipole interactions . -
pubmed
Se ,
Zn and
Cu were measured in plasma together with
glutathione peroxidase (
GSH - Px ) activity , and lipid peroxidation (
thiobarbituric acid - reactive substances -- TBARS ) . -
pubmed
A radiophotoluminescent ( RPL ) glass rod dosimeter ( GRD ) and a small active volume p - type
silicon diode detector ( stereotactic field detector ,
SFD ) were used for the measurement of Gamma - Knife output factors . -
pubmed
The immunophenotypic profile of the two subtypes was indistinguishable concerning the presence or absence of these
antigens , including the absence of reactivity for the
HLA - DR antigen . -
pubmed
For both R689Q and M731 T , sensitivity to
vanadate inhibition is decreased , suggesting that the steady - state E(1 ) & lt;==> ; E(2 ) poise of the
enzyme is shifted toward E(1 ) . -
pubmed
It is suggested that the disease phenotype is the consequence of lowered molecular activity of the
alpha2 pump isoform due to either decreased K(+ ) affinity ( T345A ) or catalytic turnover ( R689Q and M731 T ) , thus causing a delay in extracellular
K (+ ) clearance and/or altered localized
Ca ( 2 + ) handling / signaling secondary to reduced activity in colocalized
Na(+)/
Ca ( 2 + ) exchange . -
pubmed
In an example application ,
PG-594 was added to simulated milk samples to study effects of heat and
beta - lactoglobulin (
beta - LG ) on the distribution of PG . -
pubmed
Before heating , about one - third of the
PG-594 remained soluble in the whey fraction ( supernatant ) whereas the
rest became associated with the
casein micelle . -
pubmed
Heat - induced
PG-594 binding to micelles in whey - protein - free systems was evidenced by a decrease of
PG-594 from 31 to 15 % in the whey fraction accompanied by an increase of
PG-594 from 69 to 85 % in
casein micelle fractions . -
pubmed
Incubation of the
casein micelles with the reducing agent
beta - mercaptoethanol revealed that
disulfide bonds formed between PG and
casein or between PG and
casein - bound beta - LG are the mechanisms for heat - induced PG binding to
casein micelles . -
pubmed
Theoretically important findings are : 1 ) when heated , serum PG is capable of covalently binding to micellar
casein or complexing with beta - LG in whey and then coadhering to micelles , and 2 ) PG that associated with micellar
casein through
lysine binding sites before heating is capable of developing heat - induced
disulfide bonds with
casein . -
pubmed
The fact that the main mediators involved are
sulfidoleukotrienes (
LTC4 ,
LTD4 ,
LTE4 ) and that the drugs responsible all inhibit
cyclooxygenase-1 (
COX-1 ) , pointed to a pharmacogenetic abnormality of
arachidonic acid metabolism . -
pubmed
The splanchnic bed produces
cortisol at rates approximating extraadrenal tissues by converting
cortisone to
cortisol via the
11beta - hydroxysteroid dehydrogenase ( 11beta - HSD ) type 1 pathway . -
pubmed
Fasting
glucose and
insulin concentrations did not differ on the meal and saline study days . -
pubmed
Glucose and
insulin concentrations increased after meal ingestion , peaking at 11.0 + /- 1.0 mmol / l and 451 + /- 64 pmol / l , respectively , at 45 min , then fell to baseline thereafter . -
pubmed
In contrast ,
glucose and
insulin concentrations slowly fell to 5.1 + /- 0.1 mmol / l and 27 + /- 6 pmol / l during the 6 h of observation on the saline study day . -
pubmed
We conclude that ingestion of a mixed meal does not alter either splanchnic
cortisol production or the conversion of
D4 cortisol to
D3 cortisol or , therefore by implication , flux via the splanchnic
11beta - HSD type 1 pathway . -
pubmed
Enantiomer separation of
amino acids in immunoaffinity micro LC -
MS . -
pubmed
Chiral immunoaffinity microbore columns were directly interfaced with
MS detection , and the effect of column length and temperature on the enantiomer separation of a number of underivatized
aromatic and aliphatic amino acids was investigated utilizing an
antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti -
D - amino acid antibody onto
silica . -
pubmed
The stronger affinity of the
antibody towards aromatic and bulky
amino acids allowed separation of such analytes in a 0.75 x 150 mm column , while an increase in column length enabled separation of more weakly bound compounds . -
pubmed
For the first time , simultaneous enantiomer separation of mixtures of
amino acids was achieved on
antibody - based chiral stationary phases using extracted ion chromatograms . -
pubmed
Synthesis ,
dihydrofolate reductase inhibition , antitumor testing , and molecular modeling study of some new
4 ( 3H )- quinazolinone analogs . -
pubmed
After blending the products with
polyurethane ( PU ) and casting the result as film , the
PEO segments migrated to the surface of the blend and the
PCL segments acted as an anchor to fix the co - polymer on PU matrix , while the
PEO segments provided PU the hydrophibility to prevent the
fibrinogen adsorption on PU . -
pubmed
Biosynthesis of
isoprenoids : studies on the mechanism of
2C - methyl - D - erythritol-4 - phosphate synthase . -
pubmed
2C - Methyl - D - erythritol-4 - phosphate synthase , encoded by the
ispC gene ( also designated
dxr ) , catalyzes the first committed step in the
nonmevalonate isoprenoid biosynthetic pathway . -
pubmed
Hydroxyacetone , the putative second fragment expected from the
retroaldol cleavage , was not incorporated into the
enzyme product . -
pubmed
In contrast to other reports ,
IspC did not catalyze the isomerisation of
1 - deoxy - D - xylulose 5 - phosphate to give
1 - deoxy - L - ribulose 5 - phosphate under any conditions tested . -
pubmed
The patient carrying the last duplication presents sex reversal ,
Peters ' anomaly and
renal cysts and the duplication is located 4Mb away from the
HSD17B1 gene , coding a key enzyme of
testosterone biosynthesis . -
pubmed
Apixaban , an oral , direct inhibitor of
activated Factor Xa . -
pubmed
Apixaban is an oral , direct
Factor Xa inhibitor that is being developed by Bristol - Myers Squibb Co and Pfizer Inc.
Apixaban is currently undergoing phase III clinical trials for
cerebrovascular ischemia ,
deep vein thrombosis and
lung embolism , and phase II clinical trials for
coronary artery disease . -
pubmed
This was performed by direct analysis of the ability of
U1 to bind to wild - type and mutant donor sites , by engineering an in vitro splicing system to directly evaluate the functional importance of
U1 snRNA base pairing with the exon 29 donor site , and by coexpression of mutant
U1 snRNP
molecules to try to rescue exon 29 inclusion in vivo . -
pubmed
The present study investigates the consequences of cigarette smoke exposure on ciliary function and transepithelial
chloride (
Cl (- ) ) secretion , a major determinant of
ASL . -
pubmed
Effects on ciliary beat frequency (
CBF ) and
Cl (- ) secretion were investigated using pharmacologic manipulation . -
pubmed
Forskolin - stimulated
CBF was also significantly reduced when acutely exposed to CSC ( 5.64 + /- 0.06 Hz vs. control 7.15 + /- 0.18 Hz ) . -
pubmed
For the rapid detection of the
ethanol , pH and
rest sugar in red wine , infrared ( IR ) spectra of 44 wine samples were analyzed . -
pubmed
The models were used to estimate the contents of
ethanol , pH and
rest sugar in red wine samples for both in calibration set and predicted set . -
pubmed
The results indicate that the r and RMSEP for the prediction of
ethanol content , pH and
rest sugar content are 0.953 , 0.983 and 0.994 , and 0.161 , 0.017 and 0.181 , respectively . -
pubmed
The addition of immunosuppressive therapy , such as
rituximab , to
ITI may improve outcomes , although the optimal approach to combined
ITI / immunosuppression has not been established . -
pubmed
Estrogen receptors alpha (
ER - alpha ) and beta (
ER - beta ) play distinct biological roles in onset and progression of hormone - responsive
breast cancer , with
ER - beta exerting a modulatory activity on
ER - alpha - mediated
estrogen signaling and stimulation of cell proliferation by mechanisms still not fully understood . -
pubmed
This probably occurs by correcting
diabetes - linked increased clearance of
thiamine , maintaining activity and expression of
thiamine pyrophosphate - dependent enzymes that help counter the adverse effects of high
glucose concentrations - particularly
transketolase . -
pubmed
We have conducted a site - specific saturation mutagenesis study of H181 and H184 of
flavoprotein pentaerythritol tetranitrate reductase (
PETN reductase ) to probe the role of these residues in substrate binding and catalysis with a variety of
alpha , beta - unsaturated alkenes . -
pubmed
In addition , many mutants exhibited a switch in reactivity to predominantly catalyse
nitro reduction , as opposed to
C C reduction . -
pubmed
The multiple binding conformations of
hydroxy substituted nitro - olefins in
PETN reductase were examined by using both structural and catalytic techniques . -
pubmed
Serum
uric acid ( UA ) and
creatine phosphokinase were evaluated . -
pubmed
The levels of
creatine phosphokinase and
F ( 2 )- isoprostanes decreased after exercise . -
pubmed
Prevention of
benzyl alcohol - induced aggregation of
chymotrypsinogen by PEGylation . -
pubmed
Herein we investigated the efficiency of PEGylation ( attachment of
poly ( ethylene glycol ) ) to prevent
benzyl alcohol - induced aggregation of the model protein
alpha - chymotrypsinogen A (
aCTgn ) . -
pubmed
KEY FINDINGS : When the model
protein was reconstituted in buffer containing 0.9 %
benzyl alcohol , copious amounts of buffer - insoluble aggregates formed within 24 h ( & gt;10 % ) . -
pubmed
Benzyl alcohol - induced aggregation was completely prevented when two or five molecules of
PEG with a molecular weight of 5000 Da were attached to the
protein , whereas two or four molecules of bound 700 Da
PEG were completely inefficient in preventing aggregation . -
pubmed
Involvement of
visfatin in
palmitate - induced upregulation of
inflammatory cytokines in hepatocytes . -
pubmed
Visfatin - specific small interfering RNA significantly decreased the
palmitate - induced mRNA expression and protein synthesis of
interleukin-6 and
tumor necrosis factor - alpha . -
pubmed
Finally , by using the immunoabsorption method , binding partner proteins of two proteins residing in the interiors ,
PSD-95 and
alpha - tubulin , and those of two proteins residing in the peripheral regions ,
elongation factor-1alpha and
calcium ,
calmodulin - dependent protein kinase II alpha subunit , of cerebral and cerebellar PSDs were identified . -
pubmed
A retrospective chart review was conducted on all patients treated with
warfarin for a minimum of 14 days within a Long - Term Care ( LTC ) facility to compare Time within Therapeutic Range (
TTR ) between staff treated patients versus pharmacist treated patients . -
pubmed
Design and synthesis of
8 - substituted benzamido - phenylxanthine derivatives as
MAO - B inhibitors . -
pubmed
We designed and synthesized a class of
8 - substituted benzamido - phenylxanthine derivatives as
MAO - B inhibitors . -
pubmed
The laboratory investigations showed a significant drop in
hemoglobin and increased serum
bilirubin . -
pubmed
We identified that osmotic stress induces the phosphorylation of
Raptor at
Ser-696 ,
Thr-706 , and
Ser-863 using liquid chromatography - tandem mass spectrometry . -
pubmed
AP-3 binds to a
PAR1 cytoplasmic tail - localized
tyrosine - based motif and mediates
PAR1 lysosomal degradation independent of ubiquitination . -
pubmed
The ( I ) layers in the
sesqui - dioxane and
sesqui - benzene solvates are isostructural and unrelated to those in the hemi -
MEK ,
mono - benzene and
mono - toluene solvates . -
pubmed
Rods of one kind are similar to analogous rods in the
sesqui - dioxane and the
sesqui - benzene solvates , whereas rods of the other kind are only remotely related to rods in the hemi -
MEK solvate . -
pubmed
The ability of
IGFBP-3 to modulate the balance between pro - apoptotic and pro - survival
sphingolipids by regulating
sphingosine kinase 1 and
sphingomyelinases may be integral to its role at the crossroads between cell
death and survival in response to a variety of stimuli . -
pubmed
Site-3 toxins bind primarily to the extracellular loops connecting transmembrane helical segments
S1 -
S2 and
S3 -
S4 in Domain 4 (
D4 ) and
S5 -
S6 in Domain 1 (
D1 ) and slow fast - inactivation of voltage - gated
sodium channels . -
pubmed
Scorpion alpha - toxin binding impedes D4S4 segment movement during inactivation since the modification rates of R3C in
hNav1.4 with
methanethiosulfonate (
CH3SO2SCH2CH2R , where R =
- N ( CH3 ) 3 ( + ) trimethylammonium ,
MTSET ) and
benzophenone-4 - carboxamidocysteine methanethiosulfonate (
BPMTS ) were slowed ~10 - fold in toxin - modified channels . -
pubmed
The
TARP gamma-8 isoform , which is highly expressed in the hippocampus , has a unique , long C - terminal domain with five distinct regions : two
glycine - rich regions , a
serine /
arginine - rich region , a
proline /
alanine ( P / A ) rich region , and a
PSD-95 /
Dlg /
ZO-1 ( PDZ ) binding motif . -
pubmed
In addition , the
GST - gamma-8 C - terminal tail ( residues 353 - 414 ) fusion protein containing the P / A - rich region bound to purified
calcineurin in a
Ca ( 2 + ) /
calmodulin - dependent manner , whereas
GST - gamma-8 with a deletion of the P / A - rich region did not . -
pubmed
Our findings further suggest that the competition among various emission centers (
bright edge states ) and traps dominates the optical properties of these fluorescent
carbon nanomaterials . -
pubmed
Significance of
osteopontin in the sensitivity of
malignant pleural mesothelioma to
pemetrexed . -
pubmed
However , the mechanism of the sensitivity of
MPM to
PEM remains unclear . -
pubmed
We analyzed the antitumor effects of
PEM in six
MPM cell lines by
MTS assay . -
pubmed
Pathway analysis revealed that
osteopontin (
SPP1 /
OPN ) was an important target in
PEM sensitivity . -
pubmed
PEM treatment with
SPP1 /
OPN knockdown inhibited the
PEM - induced cell growth - inhibitory effect in
PEM - sensitive cells . -
pubmed
Expression of
SPP1 /
OPN and
AKT phosphorylation significantly decreased after
PEM treatment of the
PEM - sensitive cells . -
pubmed
PEM has antitumor effects in
MPM cells dependent on
SPP1 /
OPN overexpression resulting in
AKT activation . -
pubmed
Our results suggest that
SPP1 may be used as a single predictive biomarker of the effectiveness of
PEM treatment in
MPM . -
pubmed
The voltage - gated
sodium channel
TPC1 confers endolysosomal excitability . -
pubmed
The organelles have a previously uncharacterized , depolarization - activated , noninactivating
Na (+ ) channel (
lysoNaV ) . -
pubmed
Thus ,
TPC1 is a member of a new family of voltage - gated
Na (+ ) channels that senses pH changes and confers electrical excitability to organelles . -
pubmed
UNLABELLED : PURPOSES /
AIM : Glucocorticoid
steroids can induce expression of
PPARgamma gene and enhance adipogenesis by bone marrow mesenchymal stem cells ( BMSCs ) , which may result in
osteonecrosis of the femoral head . -
pubmed
The purpose of this study was to evaluate the preventive effects of siRNA targeting
PPARgamma gene on
steroid - induced adipogenesis and
osteonecrosis . -
pubmed
The resulting mature form ( M(WT ) ) containing the catalytic domain and the
PkdD showed optimum azocaseinolytic activity at 3 to 3.5 M
NaCl , demonstrating salt - dependent stability . -
pubmed
Activated platelets , in response to
tissue damage , induce a proinflammatory program involving the aberrant expression of
cyclooxygenase ( COX )- 2 , which leads to increased tissue concentrations of the proinflammatory and protumorigenic
prostaglandin E2 . -
pubmed
Low - dose
aspirin acts as an antiplatelet agent by causing an irreversible inactivation of platelet
COX-1 activity and the synthesis of
thromboxane A2 . -
pubmed
In this review we first discuss ion channels and transporters that function at the plasma membrane of melanocytes ; in the second part we consider
ion transport across the membrane of intracellular organelles , with emphasis on
melanosomes . -
pubmed
CONCLUSIONS :
Letrozole and
everolimus synergistically inhibit the proliferation and induce cell apoptosis by inhibiting the cell signal pathway
PI3K /
Akt /
mTOR and inducing cell apoptosis , thus showing a promising anti -
tumor effectiveness . -
pubmed
Reported here is the use of stable isotope labeling with
amino acids in cell culture ( SILAC ) and
pulse proteolysis (
PP ) for detection and quantitation of protein - ligand binding interactions on the proteomic scale . -
pubmed
Increasing the substitute of
carboxylic groups on CM - beta -
CD was propitious to the formation of
Ag nanoclusters and the stability of the produced
silver nanoclusters was greatly improved . -
pubmed
N - Acylethanolamine acid amidase (
NAAA ) is a
cysteine amidase belonging to the
N - terminal nucleophile ( Ntn ) hydrolases that primarily degrades anti - inflammatory and analgesic lipid amide
palmitoylethanolamide . -
pubmed
In this chapter , we review our contribution to ( i ) the determination of the reaction mechanism of
amide hydrolysis catalyzed by
cysteine Ntn - hydrolases and ( ii ) the discovery and optimization of active - site - directed inhibitors of
NAAA characterized by a
beta - lactone warhead . -
pubmed
At
H6 , parameters reflecting macrocirculation were not associated with mortality , whereas higher arterial
lactate and mottling score were associated with death . -
pubmed
The mean
hemoglobin concentration was lower in women compared to men ( 11.7 vs. 12.6 g / dl , p & lt;= 0.0001 ) , while the blood
urea and
creatinine levels were higher in men ( p & lt ; 0.0001 ) . -
pubmed
Erratum for :
Eriocitrin and
Apigenin as New
Carbonic Anhydrase VA Inhibitors from a Virtual Screening of Calabrian Natural Products . -
pubmed
In contrast to PFO- subjects , PFO+ subjects had 1 ) no improvement in A - aDO2 at
rest and during exercise at ALT16 compared with
ALT1 , 2 ) no significant increase in resting alveolar ventilation , or alveolar Po2 , at ALT16 compared with
ALT1 , and consequently had 3 ) an increased arterial Pco2 and decreased arterial Po2 and arterial
O2 saturation at
rest at ALT16 . -
pubmed
Furthermore , PFO+ subjects had an increased incidence of
acute mountain sickness (
AMS ) at
ALT1 concomitant with significantly lower peripheral
O2 saturation ( SpO2 ) . -
pubmed
Bromodeoxyuridine birth dating experiments in combination with immunohistochemical analysis using layer - specific markers showed that
Dab2IP is important for proper positioning of a subset of layer
II -
IV neurons in the developing cortex . -
pubmed
Here we present evidences of the effect of
Thioflavin T (
ThT ) , a worldwide used fluorescent dye to monitor amyloid growth , on the
Abeta ( 1 - 40 ) conformation , stability and aggregation . -
pubmed
In particular
ThT interactions with specific
Abeta(1 - 40 ) residues promote a rigid partially - folded conformation which shifts the balance between different species in solution toward a more aggregation - prone ensemble of peptides , leading to aggregation . -
pubmed
KIM-1 /
TIM-1 is a
phosphatidylserine receptor that is expressed on epithelial cells and can transform the cells into phagocytes . -
pubmed
Desflurane preconditioning protects human umbilical vein endothelial cells against
anoxia / reoxygenation by upregulating
NLRP12 and inhibiting non - canonical
nuclear factor - kappaB signaling . -
pubmed
HUVECs were pre - conditioned with
desflurane ( 1.0 MAC ) for 30 min , followed by a 15 - min washout , then exposed to 60 min
anoxia and 60 min reoxygenation ( A / R ) , and incubated with 10 ng / ml
tumor necrosis factor ( TNF )- alpha for 60 min . -
pubmed
Desflurane preconditioning attenuated the effects of A / R and/or A / R plus
TNF - alpha on cell viability , decreasing the levels of
Smac and enhancing the levels of of
cIAP1 ( P<0.05 ) . -
pubmed
Preconditioning with
desflurane also enhanced the mRNA levels of
interleukin ( IL )- 10 and
NLRP12 in the cells exposed to A / R by 2.40- and 2.16 - fold , respectively . -
pubmed
Desflurane preconditioning enhanced HUVEC survival and protected the cells against
A / R injury , and our results suggested that this process involved the upregulation of
NLRP12 and the inhibition of non - canonical
NF - kappaB signaling . -
pubmed
This study aimed to establish the sequential static and static - dynamic supercritical
carbon dioxide (
SDCO2 ) fractionation conditions to obtain a higher yield and desired chitosan with lower polydispersity index (
PDI ) and higher degree of deacetylation (
DD ) . -
pubmed
Despite many studies on the effects of perinatal
Bisphenol A (
BPA ) exposure on the brain , its effects on brain
estrogen receptor (
ERalpha ) expression during puberty remain unclear . -
pubmed
We used electrospray ionization - time of flight mass spectrometry to evaluate the degree of posttranslational modification of the entire
HSA molecule that was associated with disease development and found that the fraction of
Cys34 - cysteinylated
HSA (
Cys - Cys34 - HSA ) , a major form of oxidative modification , varied substantially among the
albumin preparations . -
pubmed
These findings suggest that the
Cys - Cys34 - HSA fraction , as estimated by electrospray ionization - time of flight mass spectrometry can be used as a predictive marker for the functional impairment of
albumin preparations and that it would be preferable to use
albumin preparations with higher contents of functionally effective
albumin that correspond to a lower degree of cysteinylation of
Cys34 in clinical practice . -
pubmed
Pittsburgh sleep quality index ( PSQI ) , snore outcome survey ( SOS ) , epworth
sleepiness score ( ESS ) , the lowest arterial
oxygen saturation ( LSaO2 ) and AHI , time and ratio of slow wave sleep (
SWS ) stage and rapid eye movement ( REM ) stage were applied to comparing the curative effect between pre - operation and
post - operation periods . -
pubmed
BACKGROUND /
AIM : There are controversial results regarding the relationship of serum
25 hydroxy vitamin D [
25 ( OH ) D ] and
ulcerative colitis (
UC ) activity . -
pubmed
The alignment of
SpA sequences enabled the clustering of several isoforms as a result of non - randomly distributed
amino acid variations , located in two clusters of polymorphic sites in domains D to B and Xr ( a ) . -
pubmed
In this report , the heterologous production of
salicylate (
SA ) is the basis for metabolic extension to
salicylate 2 - O - beta - d - glucoside (
SAG ) , a natural product implicated in plant - based defense mechanisms . -
pubmed
When combined ,
SA and
SAG production titers reached ~0.9g / L and ~2.5g / L , respectively . -
pubmed
The
SAG compound was then tested for anti - inflammatory properties relative to
SA and
acetylsalicylate (
aspirin ) . -
pubmed
The risk for
PD in these groups does not linearly increase with
glucosylceramide (
GC ) accumulation or with acid
beta - glucocerebrosidase (
GCase ) activity . -
pubmed
Ambroxol hydrochloride , a pharmacological chaperone , which reduces endoplasmic reticulum (
ER ) stress induced by accumulation of mutant misfolded GCase could serve as such an agent . -
pubmed
A3F and
A3 G are
deoxycytidine deaminases that inhibit HIV-1 replication by inducing
guanine - to -
adenine hypermutation through deamination of
cytosine to form
uracil in minus - strand DNA . -
pubmed
Using size exclusion chromatography , rotational anisotropy , and in vitro deamination assays , we demonstrate that
A3F promotes
A3 G deamination activity by forming an
A3F / G hetero - oligomer in the absence of RNA which is more efficient at deaminating
cytosines . -
pubmed
After
APOBEC3 enzymes are encapsidated into virions , they deaminate
cytosines in minus - strand DNA , which forms promutagenic
uracils that induce transition mutations or proviral DNA degradation . -
pubmed
plantarum ST8Sh to represent a potential probiotic candidate , including survival in the presence of low levels of pH and high levels of ox bile , production of
beta - galactosidase ,
bile salt deconjugation , high level of hydrophobicity , functional auto- and co - aggregation properties , and adhesion to cell lines . -
pubmed
Lowering
cAMP in cystic tissues through the inhibition of the
type-2 vasopressin receptor (
V2R ) constitutes a validated strategy to reduce
disease progression . -
pubmed
Mambaquaretin-1 is a full antagonist of the
V2R activation pathways studied :
cAMP production ,
beta - arrestin interaction , and
MAP kinase activity . -
pubmed
Mambaquaretin-1 interacts selectively with the
V2R through its first loop , in the same manner that aprotinin inhibits
trypsin . -
pubmed
Mambaquaretin-1 represents a promising therapeutic agent against
PKDs . -
pubmed
This data demonstrates that
beta1 integrin is required for the maintenance of
glucose homeostasis through postnatal beta - cell function and expansion . -
pubmed
Genuine
splenic artery aneurysm rupture treated by
N - butyl cyanoacrylate and metallic
coils under resuscitative endovascular
balloon occlusion of the aorta . -
pubmed
We used 3.9x10 - 4 - 0.1 % HD , PD or
DD , 3.9x10 - 4 - 0.0125 % PD , 7.8x10 - 5 - 0.02 %
PHMB or CH concentration for 24 h to determine viability ( Cell Proliferation Kit XTT ) , proliferation ( Cell Proliferation ELISA BrdU kit ) , and migration using
wound healing assay . -
pubmed
HCEC proliferation AUC , comparing PD with
PHMB , CH ,
DD , HD ( p & lt;= 0.016 ; PD worse for all ) and keratocyte proliferation AUC , comparing
PHMB with HD , PD ( p = 0.004 ; p = 0.002 ;
PHMB better for both ) , CH with HD , PD ( p & lt;= 0.001 ; CH better for both ) and DD with PD ( p = 0.043 ; DD better ) differed significantly . -
pubmed
Keratocyte migration AUC comparing PD with control ,
PHMB , CH ,
DD and HD differed significantly ( p & lt;= 0.012 ; PD worse for all ) . -
pubmed
In conclusion , our findings revealed that selective loss of neuronal
AR actions impacts the
kisspeptin /
GnRH /
LH cascade leading to compromised ovarian follicle dynamics . -
pubmed
AIMS : This study describes differences in the partial pressures of exhaled
nitric oxide ( PeNO ) between subjects fully acclimatized (
ACC ) to 5300 m and those who have just arrived to high altitude . -
pubmed
In addition , systolic pulmonary artery pressure (
sPAP ) and arterial
oxygen saturation ( SaO2 ) were measured in all participants . -
pubmed
RESULTS :
PeNO levels on day 1 were significantly higher in the non - ACC versus
ACC cohort ( 8.7 + - 3.5 vs. 3.9 + - 2.2 nmHg , p = 0.004 ) . -
pubmed
As the non - ACC group remained at altitude ,
PeNO levels fell and were not different when compared with those of the
ACC group by day 9 ( 5.9 + - 2.4 vs. 3.9 + - 2.2 nmHg , p = 0.095 ) . -
pubmed
TNF - alpha ,
interleukin-1 (
IL-1 ) ,
adiponectin ( ADP ) ,
nitric oxide (
NO ) and
total nitric oxide synthase (
NOS ) levels were determined by enzyme linked immunosorbent assay ( ELISA ) ,
myeloperoxidase (
MPO ) activity was determined by colorimetric method ,
superoxide dismutase (
SOD ) activity was determined by
hydroxylamine method ,
malondialdehyde (
MDA ) was determined by
thiobarbituric acid colorimetric method , and
catalase (
CAT ) activity was determined by UV spectrophotometry method . -
pubmed
The inflammatory reaction in
pioglitazone intervention groups was significantly reduced ,
TNF - alpha and
IL-1 levels in serum and myocardium of DM+
PGZ 4 mg and 8 mg groups were significantly decreased as compared with those of DM group [ serum :
TNF - alpha ( ng / L ) was 268.33 + -46.57 , 261.34 + -33.73 vs. 331.40 + -69.05 , myocardium :
TNF - alpha ( ng / L ) was 144.72 + -26.90 , 139.59 + -14.59 vs. 177.48 + -27.40 ; serum :
IL-1 ( ng / L ) was 24.40 + -2.56 , 23.35 + -3.13 vs. 30.08 + -5.44 , myocardium :
IL-1 ( ng / L ) was 21.26 + -2.85 , 20.54 + -2.75 vs. 24.78 + -3.60 , all P & lt ; 0.05 ] , and
ADP levels were significantly increased [ serum ( mug / L ): 19.64 + -8.85 , 20.54 + -7.47 vs. 15.45 + -3.06 , myocardium ( mug / L ): 10.31 + -2.22 , 11.49 + -3.42 vs. 7.76 + -1.77 , all P & lt ; 0.05 ] , and the mRNA expressions of
TNF - alpha and
TLR4 in myocardium were significantly decreased (
TNF - alpha mRNA : 0.15 + -0.05 , 0.14 + -0.06 vs. 0.25 + -0.09 ;
TLR4 mRNA : 0.57 + -0.17 , 0.40 + -0.18 vs. 0.75 + -0.35 , all P & lt ; 0.05 ) . -
pubmed
Compared with the DM group ,
SOD and
NO levels in serum and myocardium were significantly increased in DM+
PGZ 4 mg and 8 mg groups [ serum :
SOD ( U / L ) was 571.39 + -40.85 , 609.28 + -54.47 vs. 535.10 + -37.08 , myocardium :
SOD ( U / mg ) was 55.74 + -8.12 , 53.60 + -9.87 vs. 42.26 + -12.34 ; serum :
NO ( mumol / L ) was 2.95 + -0.51 , 2.99 + -0.43 vs. 2.03 + -0.78 , myocardium :
NO ( nmol / mg ) was 1.95 + -0.37 , 2.11 + -0.26 vs. 1.56 + -0.33 , all P & lt ; 0.05 ] , the serum
MDA levels were significantly decreased ( mumol / L : 20.11 + -2.34 , 19.70 + -2.02 vs. 23.07 + -3.06 , both P & lt ; 0.05 ) , while no significant effect on
CAT . -
pubmed
CONCLUSIONS : 4 mgxkg-1xd-1
pioglitazone could activate
PPARgamma -
TLR4 -
TNF - alpha targeted pathway , thus inhibit inflammatory and oxidative stress factors expression , and down - regulate
hyperglycemia induced myocardium inflammatory and oxidative stress level , but the effect did not show a dose dependent manner . -
pubmed
RuBisCO large subunit gene primers for assessing the
CO2 - assimilating planktonic community structure in Jiaozhou Bay , China . -
pubmed
The protein coding genes (
rbcL /
cbbL /
cbbM ) for
RuBisCO large subunit , the most abundant protein on earth that drives biological
CO2 fixation , were considered as useful marker genes in characterizing
CO2 - assimilating plankton . -
pubmed
In this study , six different
rbcL /
cbbL /
cbbM primers were employed to screen clone libraries to identify
CO2 - assimilating plankton in Jiaozhou Bay . -
pubmed
Bay-11 - 7082 also inhibited LPS - induced increases of ROS and
Nrf2 expression . -
pubmed
Background :
Bevacizumab is a recombinant humanised monoclonal antibody to
vascular endothelial growth factor shown to improve survival in advanced
solid cancers . -
pubmed
BRAF mutation positivity trended towards better OS with
bevacizumab ( P = 0.21 ) . -
pubmed
BRAF mutation status may predict for poorer OS untreated and potential benefit from
bevacizumab . -
pubmed
The review presents general principles for choosing optimal conditions for
ecdysteroid separation , identification , and isolation using HPLC / TLC techniques in RP ,
NP - HILIC or
NP modes . -
pubmed
Thus , comprehensive overview of
MS spectral parameters of
ecdysteroids is provided . -
pubmed
Furthermore , the first two genes of the Leloir pathway for
d - galactose catabolism - encoding
d - galactose 1 - epimerase and
galactokinase - are induced more rapidly by
l - arabinose than by
d - galactose eventhough deletion mutants thereof grow as well as a wild type
strain on the
pentose . -
pubmed
d - Galactose 1 - epimerase is hyperinduced by
l - arabinose ,
d - xylose and
l - arabitol but not by
xylitol . -
pubmed
nidulans ,
l - arabinose and
d - xylose - both requiring
NADPH for their catabolisation - actively promote the
enzyme infrastructure necessary to convert
beta - d - galactopyranose via the Leloir pathway with its alpha - anomer specific enzymes , into
beta - d - glucose-6 - phosphate ( the starting substrate of the oxidative part of the
pentose phosphate pathway ) even in the absence of
d - galactose . -
pubmed
Different SDs of APG were prepared by microwave , melted and kneaded technology using
pluronic - F127 (
PL ) as a carrier . -
pubmed
Significant enhancement in the dissolution rate and oral absorption of
APG from
SD suggested that developed SD systems can be successfully used for oral drug delivery system of APG . -
pubmed
In this study , we obtained unprecedented
AFM images of the
Na , K - ATPase ( NKA ) pump after being reconstituted into
DPPC and
DPPC :
DPPE liposomes . -
pubmed
AFM data suggest that
DPPC - NKA proteoliposomes are also rightside - out protein orientated , where the protrusions have an average height of 2.1 nm , while for
DPPC :
DPPE -
NKA proteoliposomes , the majority of the protein reconstituted should be inside - out orientated , where the protrusions ' average height is 0.5 nm . -
pubmed
Materials and Methods : Bacteria were cultured on a Yersinia Selective Agar medium ( "
CIN - agar " ) at 28 C for 24 h. The microorganisms were grown in meat - peptone broth with 1.0 %
glucose to measure the absolute values of the optical density of the culture . -
pubmed
The specimens were examined with stereoscopic microscopy " BIOMED MS-1 Stereo " ( Russia ) and scanning
electron microscope "
TM 3030 plus " ( Holland ) . -
pubmed
Factors Associated with the Need for
Insulin as a Complementary Treatment to
Metformin in
Gestational Diabetes Mellitus . -
pubmed
Those treated with
metformin were compared with those who , in addition to
metformin , also needed the combination with
insulin . -
pubmed
Of these , 366 ( 77.05 % ) were submitted to single therapy with
metformin , and 109 ( 22.94 % ) needed
insulin as a complementary treatment . -
pubmed
CONCLUSION :
Obesity resulted in an increased chance of the mother needing
insulin as a complementary treatment to
metformin , while FG & lt ; 90 mg / dL and primiparity were protective factors . -
pubmed
A large number of randomized clinical trials and metanalyses provided strong evidence about therapeutic strategies acting on
glucose metabolism such as
GLP-1 RA and SGLT2i and about lipid - lowering treatment such as PCSK9i and
Icosapent Ethyl . -
pubmed
Interestingly , the associations between infant 's fasting
glucose at age 6 and
MYL6 ,
MYH11 ,
TNNT3 ,
TPM2 ,
CXCL2 , and
NCAM1 were still relevant after correcting for multiple testing . -
pubmed
At first , a three - step , one -
pot procedure was developed to obtain reproducibly
piperazine-2,6 - diones with various substituents at the N - atoms in high yields . -
pubmed
Transformation of a
piperazine-2,6 - dione with
1,4 - dibromobut-2 - ene and
3 - halo-2 - halomethylprop-1 - enes provided
3,8 - diazabicyclo [ 3.2.1 ] octane-2,4 - dione and
3,9 - diazabicyclo [ 3.3.1 ] nonane-2,4 - dione with a
vinyl group at the C2- or a
methylene group at the
C3 - bridge , respectively . -
pubmed
Since bridging via
sulfinylimines and the one -
pot bridging with
3 - bromo-2 - bromomethylprop-1 - ene gave promising yields , these strategies will be exploited for the synthesis of novel receptor ligands bearing various substituents in a defined orientation at the
carbon bridge . -
pubmed
Their metabolic profiles were studied via HPLC - PDA - ESI -
MS /
MS and afforded a tentative characterization of 98 compounds , including free organic acids ,
phenolic acid derivatives ,
flavonoid aglycones ,
flavonoid glycosides ,
flavonoids containing
3 - hydroxyl-3 - methylglutaroyl (
HMG ) units ,
coumarin derivatives and
limonoids . -
pubmed
Column chromatography resulted in isolation of six metabolites for the first time that were identified as
nobiletin (
C1 ) ,
isosinensetin (
C2 ) ,
limonin (
C3 ) ,
4 '- demethylnobiletin (
C4 ) ,
stigmasterol - O - glucoside (
C5 ) and
hesperidin (
C6 ) . -
pubmed
In vitro studies of the anti - inflammatory activity of
DCM - L against
cyclooxygenases (
COXs ) and
5 - lipoxygenase (
5 - LOX ) enzymes revealed that
DCM - L showed higher activity than the other tested fractions . -
pubmed
Anti - inflammatory therapy with
canakinumab targeting the
IL-1beta innate immunity pathway could significantly reduce the rate of recurrent cardiovascular events than placebo . -
pubmed
Given the well - documented role of
mTOR in inhibiting autophagy , our results hence support the hypothesis that the antiaging mechanism of
TPM - based medicines might involve autophagy induction . -
pubmed
These antisera are capable of binding
125I - labeled
tubulin in homologous or heterologous combinations , demonstrating immunological similarity between the
tubulins . -
pubmed
Differences between the
tubulins were further resolved by radioimmunoassays , comparing the ability of each of the
tubulins to inhibit the binding of each
125I - labeled
tubulin to each antiserum . -
pubmed
The effectiveness of
insulin diminished as the concentration of these agonists increased , but
insulin was able to suppress by 40 % the stimulation by a maximally effective concentration of
epinephrine ( 1 muM ) . -
pubmed
The response to
glucagon ,
epinephrine , or
insulin was not dependent upon protein synthesis as
cycloheximide did not alter their effects . -
pubmed
Insulin also suppressed the stimulation by
isoproterenol of
cyclic GMP . -
pubmed
These data are the first demonstration of
insulin antagonism to the stimulation of gluconeogenesis by
catecholamines . -
pubmed
Insulin reduced
cyclic AMP levels which had been elevated by low concentrations of
glucagon or by 1 muM
epinephrine . -
pubmed
This supports the hypothesis that the action of
insulin to inhibit gluconeogenesis is mediated by the lowering of
cyclic AMP levels . -
pubmed
However , evidence is presented which indicates that
insulin is able to suppress the stimulation of gluconeogenesis by
glucagon or
epinephrine under conditions where either the agonists or
insulin had no measurable effect on
cyclic AMP levels . -
pubmed
Insulin reduced the
glucagon stimulation of gluconeogenesis whether or not extracellular
Ca2 + were present , even though
insulin only lowered
cyclic AMP levels in their presence . -
pubmed
Insulin also reduced the stimulation by
epinephrine plus
propranolol where no significant changes in
cyclic AMP were observed without or with
insulin . -
pubmed
In addition ,
insulin suppressed gluconeogenesis in cells that had been preincubated with
epinephrine for 20 min , even though the
cyclic AMP levels had returned to near basal values and were unaffected by
insulin . -
pubmed
Thus
insulin may not need to lower
cyclic AMP levels in order to suppress gluconeogenesis . -
pubmed
Oral
dexamethasone ( 8 - 12 mg ) administered over an 8 - 48 h period prior to delivery does not significantly alter
placental ACTH content . -
pubmed
These results indicate that the potencies of the
TRH analogs in inducing shaking parallel their
thyrotropin - releasing activities . -
pubmed
This indicates
dityrosine structures in the radiation - induced
collagen aggregates . -
pubmed
The data also suggest that the
EGF /
PDGF cascade branches into
mitogenic and oncogenic signals , the latter of which is required for soft agar growth and focus formation . -
pubmed
The technique was then used to screen
Fab lots for batch - to - batch consistency , and for examining the effect of 10 mM
cysteine on
papain cleavage of whole antibody . -
pubmed
Protein kinase C activators
PMA 4 - phorbol 12 - myristate 13 - acetate and
DiC8 (
1,2 - dioctanoyl - sn - glycerol ) also increased
arachidonic acid release , but the time course observed with
PMA was different from that of
IL-1 . -
pubmed
To examine the role of other
kinases in
IL-1 stimulated
arachidonic acid release , cultures were treated with H-7 (
1 -( 5 - isoquinolinesulphonyl )- 2 - methylpiperazine dichloride ) , H-8 (
N -[ 2 -( methylamino ) ethyl ]- 5 - isoquinolinesulphonamide dichloride ) ,
HA1004 (
N -( 2 - guanidoinoethyl )- 5 - isoquinolinesulphonamide hydrochloride ) , and
staurosporine . -
pubmed
IL-1 stimulation of
arachidonic acid release was blocked by H-7 , H-8 and
staurosporine . -
pubmed
Addition of H-7 at various times following
IL-1 decreased
IL-1 stimulated
arachidonic acid release , suggesting that continued
protein kinase activity was necessary for
IL-1 action . -
pubmed
Cycloheximide and
actinomycin D inhibited the stimulation of
arachidonic acid release by
IL-1 ,
PMA or
DiC8 . -
pubmed
The addition of
cycloheximide or
actinomycin D 15 - 45 min after
IL-1 also inhibited
IL-1 stimulated
arachidonic acid release , indicating that continued protein synthesis was required for
IL-1 action . -
pubmed
These clones became unresponsive to
Ag after exposure to
anti - CD3 . -
pubmed
When compared with the effect of
enflurane on transmural nerve stimulation - induced responses , exposure to
enflurane resulted in slight attenuation of the contractile responses caused by
tyramine , suggesting that
enflurane may inhibit the responses to
tyramine by interfering with an interaction between released
norepinephrine and postjunctional
alpha 1 - adrenoceptors rather than with
tyramine - induced
norepinephrine release . -
pubmed
Addition of
Ca2 + and
Sr2 + to
Ca2 + -depleted cells in the presence of
BK produced a transient increase in
Ca2 +i and
Sr2 + i . -
pubmed
Addition of
Ba2 + to
Ca2 + -depleted cells in the presence of
BK produced an increase in
Ba2 + i which continued to rise with time to a steady level . -
pubmed
The
antigen DR2 was more frequent in highly sensitized ( group C ) than in moderately sensitized ( group B ) patients with P = 0.002 , not significant after correction for the number of investigated
antigens . -
pubmed
Through a cannula inserted into a lateral cerebral ventricle a solution with a normal (
CSF , 152 mmol l-1 ) or high ( NaCSF , 1,000 mmol l-1 )
sodium ion content was infused . -
pubmed
In vitro
steroid sensitivity and
interleukin 2 synthesis in normal subjects . -
pubmed
Polymeric
actin was localized in the reticulopodia by
rhodamine -
phalloidin staining . -
pubmed
Simultaneous application of
KCN and
salicylhydroxamic acid ( an alternative
oxidase inhibitor ) rapidly blocked all movement , indicating that motility is dependent on metabolic energy and that an alternative oxidative pathway functions in allogromiids . -
pubmed
These changes were not accompanied by any changes in plasma levels of
calcitonin or
parathyroid hormone . -
pubmed
The
SH mRNA contains a single open reading frame which encodes a polypeptide of 44
amino acids with a molecular weight of 5,012 . -
pubmed
[
Cobamide composition of
aminopterin - resistant "
folatereductase " ] . -
pubmed
Rehydration as a diagnostic and therapeutic measure in
hypercalcemia including an assessment of the
calcium - lowering effect of
porcine calcitonin . -
pubmed
Effects other than the direct interaction of
Cr ( III ) with DNA are inferred , which can cause infidelity of the
DNA polymerase functions . -
pubmed
After 7 days there was no significant difference between the efficacy of the two drugs with respect to '
pain at
rest ' , '
pain on movement ' , '
tenderness ' or '
sleep disturbance ' but
fenoprofen was significantly better when considering ' limitation of movement ' and ' The physician 's assessment of the severity of the condition ' . -
pubmed
Ibuprofen did not significantly improve '
pain at
rest ' but did improve the other parameters . -
pubmed
Over a period of 3 to 12 months significant reductions in
serum LH levels ,
LH /
FSH ratios and in plasma
testosterone were observed . -
pubmed
and a 5 %
alcohol solution during four weeks evoked strong increments of the serum enzyme activities of the
aminotransferases (
GOT ,
GPT ) and the
sorbitol dehydrogenase (
SDH ) . -
pubmed
In plasma , concentrations of
leucine ,
isoleucine ,
valine and
proline were higher in Group
II in the absence of
glucose intake , than in the other groups . -
pubmed
Diethylstilbestrol (
DES ) reduces significantly the plasma levels of
testosterone ,
follicle - stimulating hormone (
FSH ) and
luteinizing hormone (
LH ) . -
pubmed
In contrast , those of
prolactin ,
dehydoepiandrosterone (
DHEA ) , and
cortisol as well as
sexual hormone binding globulin (
SHBG ) are elevated . -
pubmed
The additional administration of
bromocriptine decreases
prolactin to normal levels and further decreases the
testosterone levels . -
pubmed
The addition of
cyproterone acetate to the therapeutic regimen decreases the
cortisol levels , and all other values , including
SHBG , are not altered significantly compared to the group treated with
DES plus
bromocriptine . -
pubmed
Integrated regulation of
very low density lipoprotein triglyceride and
apolipoprotein - B kinetics in
non - insulin - dependent diabetes mellitus . -
pubmed
Turnover rates of plasma
very low density lipoprotein (
VLDL )
triglyceride (
TG ) and
apolipoprotein - B (
apo - B ) were significantly increased in age- and weight - matched groups of normolipemic and hyperlipemic mild
diabetics , and hyperlipemic moderately severe diabetics when compared with normolipemic controls . -
pubmed
The increase in
LDL and/or
VLDL remnant formation , regardless of prevailing plasma lipid levels or the severity of
diabetes , provides a source of
cholesterol which may account for the atherogeneity of this disorder . -
pubmed
Increased
hypoxia has been found after
beta 2 adrenoceptor agonists ( but not
adrenaline ) in
asthmatics . -
pubmed
Both the steady - state plasma and CSF concentrations of
clonidine were proportional to the dose ; the ratio of
CSF to plasma concentration was approximately 0.5 . -
pubmed
The failure to see any change in
CSF composition , significant spinal cord pathology , or signs of
tissue or organ toxicity emphasizes the safety of epidurally administered
clonidine at infusion rates up to 320 micrograms / hr and at infusate concentrations up to 2 mg / ml . -
pubmed
In addition , direct binding of
N - CAM to
chondroitin sulfate could be demonstrated . -
pubmed
Binding of
N - CAM to the immobilized
neurocan core protein could be inhibited with all recombinant fragments containing
chondroitin sulfate or major parts of the
mucin - like central region of
neurocan . -
pubmed
The activity of
nucleotidyl transferase that adds
CCA nucleotides to the 3 ' end of tRNA was also observed . -
pubmed
Two fluorogenic probes anneal to the target between primers for
c - erbB-2 and beta - globin genes and contain both a reporter
dye (
6 - carboxy - fluorescein ) and a quencher dye (
6 - carboxy - tetramethyl - rhodamine ) . -
pubmed
During the extension phase of the PCR cycle , the 5'-->3 ' exonuclease activity of Taq
polymerase cleaves the hybridized fluorogenic probe , resulting in an increase of fluorescence emission of the reporter
dye that is quantitative for the amount of PCR product and , under appropriate conditions , for the amount of template . -
pubmed
Transformation studies showed that an
aminoglycoside resistance gene ,
aadB , is carried on a 6.0 - kb plasmid ( pRAY ) in a clinical isolate of Acinetobacter ( strain SUN ) . -
pubmed
Diversity of the
E2P phosphoforms of
Na , K - ATPase . -
pubmed
Role of the
carboxyl - terminal
lectin domain in self - association of
galectin-3 . -
pubmed
Previous experimental results suggest that , when bound to multivalent glycoconjugates ,
galectin-3 self - associates through intermolecular interactions involving the
amino - terminal domain . -
pubmed
This mode of self - association is inhibitable by the
lectin 's
saccharide ligands . -
pubmed
In addition , biotinylated
galectin-3 bound to
galectin-3 immobilized on plastic surfaces and the binding could also be inhibited by various
saccharide ligands of the
lectin . -
pubmed
A mutant with a
tryptophan to
leucine replacement in the
carboxyl - terminal domain , which exhibited diminished
carbohydrate - binding activity , did not bind to
galectin-3C - Sepharose 4B. -
pubmed
Furthermore ,
galectin-3C formed covalent
homodimers when it was treated with a chemical cross - linker and the dimer formation was completely inhibited by
lactose . -
pubmed
Therefore ,
galectin-3 can self - associate through intermolecular interactions involving both the
amino- and the
carboxyl - terminal domains and the relative contribution of each depends on whether the
lectin is bound to its
saccharide ligands . -
pubmed
We found that the region between
amino acid 353 and 393 is critical for the proliferation signal and activation of
STAT6 , but not for
tyrosine phosphorylation of
Jaks . -
pubmed
Gadolinium chloride (
GC ) , a rare earth
lanthanide , has been used in in vivo studies to abrogate macrophage function to understand their role in tissue pathology . -
pubmed
Although alkaline
agarose electrophoresis of specific
SV40 fragments showed that
oxaliplatin formed interstrand cross - links , the levels of this lesion type were low . -
pubmed
Low
chlorophyll affects light absorptance but is unlikely to change light partitioning between
photosystems . -
pubmed
Both the minor (
GH I ) and the major (
GH II ) forms of purified
GH appeared as single bands ( M(r ) approximately 23,000 ) after
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis , as did the purified
PRL ( M(r ) approximately 24,000 ) . -
pubmed
Levodopa induces a cytoplasmic localization of
D1 dopamine receptors in striatal neurons in
Parkinson ' s disease . -
pubmed
Mutations in the
CNGB3 gene encoding the beta - subunit of the cone photoreceptor
cGMP - gated channel are responsible for
achromatopsia (
ACHM3 ) linked to chromosome 8q21 . -
pubmed
Analysis of the extracts by gas chromatography - electron capture detector (
GC -
ECD ) indicated that the herbicide
trifluralin (
2,6 - dinitro - N , N - dipropyl-4 - trifluoromethylaniline ) could be quantitatively extracted by using the
SFE system proposed with small amounts of sample . -
pubmed
After extraction of both
lipoprotein ( a ) and
low - density lipoprotein with
glycerol prior to rotary shadowing , the protein components were observed to consist of a ring of density made up of nodules of different sizes , with
apolipoprotein ( a ) and
apolipoprotein B-100 closely associated with each other . -
pubmed
However , when
lipoprotein ( a ) was treated with a
lysine analogue ,
6 - aminohexanoic acid , much of the
apolipoprotein ( a ) separated from the
apolipoprotein B-100 . -
pubmed
In
6 - aminohexanoic acid - treated preparations without
glycerol extraction ,
lipoprotein ( a ) particles had an irregular mass of density around the core . -
pubmed
In contrast ,
lipoprotein ( a ) particles treated with
6 - aminohexanoic acid in the presence of
glycerol had a long tail , in which individual kringles could be distinguished , extending from the ring of
apolipoprotein B-100 . -
pubmed
As
retinoids have been shown to inhibit the growth of
breast cancer cells , loss of the proposed
SEC14L1 retinal - binding function may contribute to breast
tumorigenesis . -
pubmed
Recessively inherited mutations in the
HFE gene are responsible for
iron accumulation in most cases of
hereditary haemochromatosis and may have a role in
HCV infection . -
pubmed
AIMS : To assess the prevalence of
HFE mutations in Scottish
HCV infected patients and to explore the effect of the carrier state on serum and liver
iron stores , and the severity of
liver disease . -
pubmed
Iron markers were assessed in serum (
ferritin ,
transferrin saturation ) and on liver biopsy ( stainable
iron , liver
iron concentration ( LIC ) and hepatic
iron index ) . -
pubmed
Carriage of
HFE mutations was not associated with any clinical , biochemical , virological , or pathological features , including accumulation of liver
iron . -
pubmed
Carriage of
HFE mutations , although frequently observed in these
HCV infected patients , does not have a role in the accumulation of
iron or the progression of
liver disease in
HCV infection . -
pubmed
The
BLM gene has 4437 bp and encodes 1417
amino acids . -
pubmed
Apoptotic and
necrotic cells were determined using
Annexin - V and
propidium - iodide staining . -
pubmed
To identify inhibitors of the intrinsic N - acetylated
alpha - linked acidic dipeptidase (
NAALADase ) activity of
prostate specific membrane antigen (
PSMA ) that may be useful for targeting imaging agents or chemotherapeutic drugs to disseminated
prostate cancer , analogs of the tetrahedral transition state for hydrolysis of the natural substrate ,
N - acetylaspartylglutamate (
NAAG ) , were synthesized . -
pubmed
We have identified nine compounds , including fluorescent and
iodine - labeled conjugates , which inhibit
NAALADase enzyme activity with IC(50)s at , or below , 120nM. -
pubmed
[
Dehydrogenases of higher
fatty acids in the isolated liver in normal and pancreatectomized animals ] . -
pubmed
The initial step in the formation of these
diacids , which is thought to be rate limiting , is omega - hydroxylation by a
cytochrome P450 ( CYP ) monooxygenase . -
pubmed
tropicalis ATCC 20336 contains a family of
CYP genes , and when ATCC 20336 or its derivatives are exposed to
oleic acid ( C(18:1 ) ) , two
cytochrome P450s ,
CYP52A13 and
CYP52A17 , are consistently strongly induced ( Craft et al . -
pubmed
CYP52A13 preferentially oxidized
oleic acid and other
unsaturated acids to
omega - hydroxy acids . -
pubmed
CYP52A17 also oxidized
oleic acid efficiently but converted shorter ,
saturated fatty acids such as
myristic acid ( C(14:0 ) ) much more effectively . -
pubmed
Both
enzymes , in particular
CYP52A17 , also oxidized
omega - hydroxy fatty acids , ultimately generating the
alpha , omega - diacid . -
pubmed
Consideration of these different specificities and selectivities will help determine which
enzymes to amplify in
strains blocked for beta - oxidation to enhance the production of
dicarboxylic acids . -
pubmed
Proliferation and osteogenic differentiation were analyzed by cell counting , an
MTT assay ,
alkaline phosphatase activity , and
total protein content . -
pubmed
CD14 - positive cells cultured in the presence of
PSK significantly increased the expression of
HLA class II antigen and
CD40 ; significantly increased the number and expression of
CD80- ,
CD86- and
CD83 - positive cells ; decreased
Fluorescein isothiocyanate (
FITC)-dextran uptake , augmented
IL-12 production ; augmented the allogeneic mixed lymphocyte reaction ; and induced antigen - specific
cytotoxicity . -
pubmed
We examined the
SOD1 to
GPX1 mRNA ratio in individual organs , as both enzymes form part of the body 's defense against oxidative stress , and because a disproportionate increase of
SOD1 to
GPX1 results in noxious
hydroxyl radical damage . -
pubmed
Comparative molecular field analysis and comparative molecular similarity indices analysis were performed on twenty five analogues of
pimarane COX-2 inhibitor to optimize their
cyclooxygenase-2 (
COX-2 ) selective anti - inflammatory activities . -
pubmed
PE - induced phosphorylation of
CREB was partially inhibited by
Ro318220 and
H89 , which were shown to be potent inhibitors of
mitogen - and stress - activated protein kinase-1 (
MSK1 ) activation , implicating the involvement of this
kinase in the response . -
pubmed
In addition , inhibition of
protein kinase A by
RpcAMP reduced phosphorylation of
CREB , suggesting that this pathway is also involved . -
pubmed
An enhanced
CBP /
phospho -
CREB complex formation was also observed , suggesting recruitment of
CBP to phosphorylated
CREB . -
pubmed
BACKGROUND :
HIV protease inhibitor
ritonavir (
RTV ) is associated with many
cardiovascular complications and causes
vascular dysfunction through oxidative stress . -
pubmed
RTV - treated arteries showed substantial reductions of
eNOS mRNA by 77 % ,
eNOS protein by 72 % , and
nitric oxide release by 37 % as compared with controls . -
pubmed
The
RTV plus
curcumin - treated vessels showed substantial increases of
eNOS and
nitric oxide levels as compared with the
RTV - treated vessels . -
pubmed
Palladium - catalyzed one -
pot synthesis of highly substituted
furans by a three - component annulation reaction . -
pubmed
Mechanism and influence factors of resonance light scattering (
RLS ) spectra of
4 -( 2 - pyridylazo )- resorcinol (
PAR ) aqueous solution were studied . -
pubmed
The spectral feature of
RLS is related to the absorption spectra of
PAR . -
pubmed
By a light polarization experiment , the
RLS of
PAR was demonstrated to be complete polarized light . -
pubmed
Plasma
RBP4 concentrations were found to be associated with sex , waist circumference , fasting plasma
glucose , and
insulin resistance . -
pubmed
Of these , sex and fasting plasma
glucose levels were found to be independent determinants of plasma
RBP4 concentration . -
pubmed
Serum samples were analyzed for
adiponectin , inflammatory markers , and
high density lipoprotein cholesterol (
HDL ) . -
pubmed
RESULTS :
Lipoatrophy was associated with higher
triglycerides ( 330 vs 133 mg / dl , p = 0.0003 ) , lower
HDL ( 33 vs 48 mg / dl , p = 0.02 ) , and a greater frequency of
hypercholesterolemia ( total
cholesterol & gt ; 200 mg / dl ; 64 % vs 23 % , p & lt ; 0.03 ) . -
pubmed
Recent advances in this area indicate that
glycosyltransferases involved in the biosynthesis of natural products have substrate flexibility regarding the
sugar donor but also , less frequently , with respect to the
aglycon acceptor . -
pubmed
BACKGROUND : Real - time 3 - dimensional echocardiography ( RT3DE ) to visualize the coronary
sinus (
CS ) was compared with multislice cardiac tomography ( MSCT ) . -
pubmed
To assess the influence of temperature on
calcium - triggered exocytosis , patch -
clamp recordings of voltage - gated L - type
Ca ( 2 + ) influx and exocytic membrane capacitance changes were performed at room ( 25 degrees C ) and physiological ( 35 - 37 degrees C ) temperatures . -
pubmed
Immunohistochemistry of 1st and 3rd trimester placenta detected
organic anion transporter 4 (
OAT4 ) and
organic anion transporting polypeptide 2B1 (
OATP2B1 , former name
OATP - B ) in cytotrophoblast membranes and at the basal surface of the syncytiotrophoblast , indicating that both transporter polypeptides are involved in placental uptake of foetal derived
steroid sulfates . -
pubmed
In contrast ,
DHEA - S uptake was only highly increased in
OAT4 ( 40 times ) , but only weakly enhanced in
OATP2B1 cells . -
pubmed
The uptake of
DHEA - S and
E ( 1 ) S by
OAT4 was partly
Na(+)-dependent ( about 50 % ) , whereas uptake of
DHEA - S by
OATP2B1 was
Na(+)-independent . -
pubmed
In contrast , the affinity of
DHEA - S towards
OATP2B1 was about 10 times lower ( K(m)>200microM ) then for
OAT4 ( K(m)=29microM ) . -
pubmed
OATP2B1 seems not to be involved in de novo synthesis of placental
estrogens but may contribute to the clearance of
estrogen sulfates from foetal circulation . -
pubmed
Pharmacological subversion of the
S1P rheostat is proving to be clinically efficacious in
multiple sclerosis , and both the scope and limitations of therapeutic modulation of the
S1P axis in immunotherapy are becoming clearer as understanding of the integrated chemical physiology of the
S1P system emerges . -
pubmed
Influence of solid - state acidity on the decomposition of
sucrose in amorphous systems
II ( effect of buffer ) . -
pubmed
blazei intake did not suppress
ACF development or crypt multiplicity induced by
DMH . -
pubmed
Because
benzodiazepines and
ethanol have similar effect signatures , it has been hypothesized that these drugs share the
gamma2 - containing GABA ( A ) receptors as a mechanism of action . -
pubmed
While baseline learning and memory were unaffected by reduction of gamma2 containing
GABA ( A ) receptors ,
ethanol dose - dependently
impaired spatial memory equally in gamma2 heterozygous knockouts and wild type littermate controls . -
pubmed
For instance , acting as an intracellular
iron carrier and protecting
MMP9 from proteolytic degradation ,
NGAL has a clear pro - tumoral effect , as has already been observed in different
tumors ( e.g. -
pubmed
Apolipoprotein E - containing lipoproteins (
LpE ) are generated in the central nervous system by glial cells , primarily astrocytes , and are recognized as key players in
lipid metabolism and transport in the brain . -
pubmed
Interestingly , a number of studies have also demonstrated that manipulating
GR function with both agonists and antagonists has an antidepressant effect , and indeed that other drugs targeting the
HPA axis and
cortisol secretion - even drugs with opposite effects on the
HPA axis - have antidepressant effects . -
pubmed
Nicotine 's effect on hyperexcitability of inflamed neurons was blocked in the presence of an
alpha ( 7 )- nicotinic acetylcholine receptor (
nAChR ) antagonist ,
methyllicaconitine , while
choline , the
alpha ( 7 )- nAChR agonist , induced a similar effect to that of
nicotine . -
pubmed
An almost fourfold increase in shelf life ( ~196 h ) was observed at the
GA - predicted optimum ( protein concentration : 6.49 mg / ml , storage temperature : 20.8 C ,
Glycerol : 10.02 % ,
NaCl : 51.65 mM and pH : 8.2 ) . -
pubmed
Changes in hormones , including
testosterone ,
estrogen ,
cortisol ,
vasopressin , and
prolactin , during the postpartum period in fathers may be biological risk factors in paternal
PPD . -
pubmed
Langmuir - Blodgett films were prepared by transferring the monolayers onto
silica substrates and were subsequently analyzed by atomic force microscopy (
AFM ) . -
pubmed
Here , through in situ high - resolution transmission electron microscopy ( HRTEM ) observations , we show that ( 1 ) in sharp contrast to what happens in bulk materials , in which plasticity is mediated by dislocation emission from Frank - Read sources and multiplication , partial dislocations emitted from free surfaces dominate the deformation of gold (
Au ) nanocrystals ; ( 2 ) the crystallographic orientation ( Schmid factor ) is not the only factor in determining the deformation mechanism of nanometre - sized
Au ; and ( 3 ) the
Au nanocrystal exhibits a phase transformation from a
face - centered cubic to a body - centered tetragonal structure after failure . -
pubmed
The Endo -
F2 and Endo -
F3 were efficient for transferring both
sialylated and
asia - lylated glycans and were highly specific for an
alpha-1,6 - fucosylated GlcNAc - peptide as acceptor for transglycosylation , showing only marginal activity with non -
fucosylated GlcNAc - peptides . -
pubmed
In contrast , we found that the commonly used
endoglycosidases such as
Endo - A and
Endo - M , which belong to
GH family 85 , were unable to take
alpha-1,6 - fucosyl - GlcNAc derivative as acceptors for transglycosylation . -
pubmed
The novel activity of Endo -
F2 and Endo -
F3 was successfully applied for a highly convergent chemoenzymatic synthesis of a full - sized
CD52 glycopeptide antigen carrying both terminal
sialic acid and core
fucose . -
pubmed
This is the first report on
endoglycosidases that are able to glycosylate
alpha-1,6 - fucosylated GlcNAc derivatives to form natural core -
fucosylated glycopeptides . -
pubmed
The roles of their many potential receptors and/or binding proteins have been more difficult to assess at the genetic level due to the complexity of membrane interactions with these large proteins , but roles for
MuSK -
LRP4 in
agrin signaling and for
integrins ,
dystroglycan , and voltage - gated
calcium channels in
laminin - dependent phenotypes have been identified . -
pubmed
The C allele rs2470152 was significantly associated with increased risk of
vertebral fractures ( P = 0.04 ) , whereas none of the
CYP19 polymorphisms showed differences in sex
steroid levels between subjects with and without
fractures . -
pubmed
Platelet adenosine diphosphate receptor antagonists :
ticlopidine to
ticagrelor - a long continuing journey . -
pubmed
Platelet adenosine diphosphate ( ADP ) receptor antagonists (
ticlopidine ,
clopidogrel ,
prasugrel , and
ticagrelor ) are a major advance in the treatment of
atherothrombotic diseases , especially
acute coronary syndromes (
ACS ) . -
pubmed
Ticagrelor is the latest non -
thienopyridine ADP receptor blocker that is potent , effective , reversible , and relatively safer as compared to
clopidogrel . -
pubmed
We have earlier shown that stimulation of
endothelin B receptors by
IRL-1620 provides significant neuroprotection at 24h following
cerebral ischemia . -
pubmed
This study was designed to determine the effect of
IRL-1620 on neurological functions ,
infarct volume , oxidative stress , and
endothelin receptors following permanent
middle cerebral artery occlusion for 7 days . -
pubmed
All effects of
IRL-1620 were blocked by
endothelin B receptor antagonist ,
BQ788 . -
pubmed
By promoting cellular
cholesterol efflux in a cell autonomous manner and through its ability to enhance HDL function in
hyperlipidemic plasma ,
apoE is now known to suppress
atherosclerosis by controlling myeloid cell proliferation , monocyte activation and their capacity to infiltrate the vascular wall . -
pubmed
Curcumin neither suppressed
p53 mRNA transcription nor protected
tumor cells against
cisplatin cytotoxicity . -
pubmed
Matrix metalloproteinase-1 (
MMP-1 ) is a member of the family of
zinc - dependent endopeptidases that are capable of degrading extracellular matrix ( ECM ) and certain non - matrix proteins . -
pubmed
The aim of this study was to compare the incidence of
BMs in the pre- and
post -
docetaxel era in a single institution in which all oncological patients are referred to one Radiotherapy and one Medical Oncology Department . -
pubmed
The year of the introduction of
docetaxel into clinical practice ( 2002 ) divided the observation period into two 9 - year periods : period 1 (
P1 ) from 1994 until 2002 (
P1 ) , and
period 2 (
P2 ) after 2002 . -
pubmed
The plasma
DOX measurements were performed by high - performance liquid chromatography with fluorescence detection , and the
CKMB levels were assayed using the CKMB Labtest kit . -
pubmed
Serum
CKMB activity increased after conventional
DOX administration but was unchanged in the
DOX - ME group . -
pubmed
Upon addition of
Pb ( 2 + ) , it could specifically bind to its aptamer to form
Pb ( 2 +)-stablized G - quadruplex and induce the aptamer strand to release from the electrode surface into solution , accompanied by the release of intercalated
MB responsible for significant signal reduction . -
pubmed
Between - group analysis did not reveal any differences except in the ratio of
malondialdehyde (
MDA)-
LDL over
apolipoprotein B-100 (
MDA - LDL /
apoB ) in
pitavastatin vs.
atorvastatin group ( -13 % vs. -0.7 % , p = 0.04 ) . -
pubmed
Within - group changes from baseline to 12 - week revealed significant increases in
OxPL /
apoB and reductions in small - dense
LDL ,
MDA - LDL , and lipoprotein - associated
phospholipase A ( 2 ) measured on circulating
apoB particles (
Lp - PLA(2)/
apoB ) in both groups and significant reductions in
OxPL /
apoAI in the
atorvastatin group . -
pubmed
The determination of
ETU and
PTU was performed by high performance liquid chromatography with diode array detection ( HPLC / DAD ) or by gas chromatography with mass spectrometry detection (
GC /
MS ) . -
pubmed
The vls system alters the sequence of the expressed
antigen by recombining gene fragments from unexpressed but divergent ' cassettes ' into the expression site ,
vlsE. -
pubmed
Using the Ion Torrent PGM sequencer , we have analyzed a cohort of 297 unrelated Omani cases and reliably identified mutations in the beta -
globin (
HBB ) gene . -
pubmed
Nicotine exposure increases the complexity of
dopamine neurons in the parainterfascicular nucleus (
PIF ) sub - region of VTA . -
pubmed
BACKGROUND : Recent publications highlight differences within the sub - regions of the ventral tegmental area ( VTA ) including the parabrachial
pigmented nucleus (
PBP ) , parainterfascicular nucleus (
PIF ) and paranigral nucleus (
PN ) in the projections to the prefrontal cortex ( PFC ) and the glutamatergic pathway . -
pubmed
RESULTS : Our results have shown that the local field potentials corresponding to the neurons located in the
PIF region of the VTA have ApEn values significantly higher ( p = 2x10 - 4 ) in the maternal
nicotine cases when compared to the saline . -
pubmed
CONCLUSION : Therefore , we speculate that the
dopamine neurons located in the
PIF sub - region of the VTA are very likely involved with the
nicotine addiction . -
pubmed
INTRODUCTION : Extracellular
adenosine triphosphate (
ATP ) stimulates vasodilation by binding to endothelial
ATP - selective
P2Y2 receptors ; a phenomenon , which is posited to be accelerated during exercise . -
pubmed
CONCLUSIONS : Oral
ATP administration can increase
post - exercise blood flow , and may be particularly effective during exercise recovery . -
pubmed
The method was further used to assess the kinetics of hyperpolarized
pyruvate -
lactate exchange acquired in vitro in whole blood and to re - analyze the previously published in vitro reaction of hyperpolarized
( 15 ) N choline with
choline kinase . -
pubmed
Secondary outcomes included admission and duration of stay in the intensive care unit ( ICU ) , ventilation assistance , time to short - acting
beta2 - agonists spaced at four hours or longer , supplemental
asthma therapy , duration of supplemental
oxygen , change from baseline in
asthma severity ,
relapse after discharge , adverse health effects and withdrawals . -
pubmed
Here we identify
USP7 as a novel interacting protein of the oligonucleotide /
oligosaccharide - binding fold of
TPP1 , which was previously known to recruit
telomerase to telomeres . -
pubmed
We identify
amino acids in
TPP1 and
USP7 that are critical for their interaction and multiple
lysines within
TPP1 that are oligo - ubiquitinated and deubiquitinated by
USP7 . -
pubmed
Lectins are proteins able to recognize
carbohydrates , without modifying their structure , via the
carbohydrate - recognition domain (
CRD ) . -
pubmed
Endothelium - derived relaxant factors , extracellular
calcium (
Ca ( 2 +) e ) and
muscarinic receptors were also evaluated as putative participants in the CRLI relaxant effect . -
pubmed
CRLI relaxant effect was blocked by
L - NAME , a nonselective inhibitor of
nitric oxide synthase (
NOS ) , and partially inhibited in a
calcium - free solution (
0Ca ) and by
atropine , but it remained unchanged in the presence of
indomethacin and
TEA . -
pubmed
In summary , our data suggest interaction between
CRLI and
muscarinic receptors located in vascular endothelial cells leading to
NOS activation triggered by a mechanism that involves
Ca ( 2 +) e along with the ability of
CRLI to interact with
heparan sulfate , a highly rated mechanoreceptor involved in
eNOS activation . -
pubmed
Films prepared from
CNF processed after pretreatment with
urea - containing microemulsions presented a more uniform fiber network and produced films with smoother surfaces compared to those based on
ethylenediamine . -
pubmed
This effect was inhibited either by antagonists of
alpha7 - nAChRs , such as
methyllycaconitine and alpha - cobratoxin , or by blocking
SK - type calcium - activated potassium ( KCa ) channels with
apamin or blocking intraterminal
ryanodine receptors with
ryanodine . -
pubmed
First , the course of activation of the
mTOR signaling pathway in
PTZ - induced
SE was examined to determine the time - point of peak activity , as reflected by phopshorylated ( p)-
mTOR /
mTOR and
p - S6 / S6 ratios . -
pubmed
Subsequently ,
pioglitazone was administrated intragastrically to investigate its effect on the
mTOR signaling pathway , through western blot and immunochemical analyses . -
pubmed
Compared with the vehicle treated -
SE group , pretreatment with
pioglitazone was associated with the loss of fewer neurons , lower levels of
IL-1beta and
IL-6 , and inhibition of the activation of the
mTOR signaling pathway . -
pubmed
Muscle glycogen , serum inflammatory
cytokines ,
aspartic aminotransferase (
AST ) ,
alanine aminotransferase (
ALT ) ,
creatinine ( CRE ) , white blood cell ( WBC ) , neutrophil ( NEUT ) , red blood cell ( RBC ) , and
hemoglobin (
HGB ) were measured . -
pubmed
Consistent with the behavioral data ,
BST204 markedly increased muscle glycogen activity and concentrations of WBC , NEUT , RBC , and
HGB . -
pubmed
Also ,
tumor necrosis factor - alpha (
TNF - alpha ) and
interleukin-6 (
IL-6 ) ,
AST ,
ALT , and CRE levels in the serum were significantly reduced in the
BST204 - treated group compared to the control group . -
pubmed
We designed an 18 - residue hybrid peptide ( PapMA ) incorporating N - terminal residues 1 - 8 of
papiliocin and N - terminal residues 4 - 12 of
magainin 2 , joined by a
proline (
Pro ) hinge . -
pubmed
Nuclear magnetic resonance experiments revealed that
PapMA contained an N - terminal alpha - helix from
Lys(3 ) to
Lys(7 ) and a C - terminal alpha - helix from
Lys(10 ) to
Lys(17 ) , with a
Pro(9 ) hinge between them . -
pubmed
OBJECTIVE : In this study , nano - biocomposite composed of
poly ( lactide - co - glycolide ) (
PLGA ) and chitosan (
CS ) were electrospun through a single nozzle by dispersing the
CS nano - powders in
PLGA solution . -
pubmed
MATERIALS AND METHODS : In this experimental study , the
PLGA /
CS scaffolds were prepared at the different ratios of 90/10 , 80/20 , and 70/30 ( w / w ) % . -
pubmed
RESULTS : H - ADSCs seeded on the matrices indicated that the
PLGA / CS composite matrix with aligned nanofibres and higher content of
CS nano - powders gave significantly better performance than others in terms of cell adhesion and proliferation rate ( P<0.05 ) . -
pubmed
RESULTS : The results indicated that
minocycline inhibited
inflammation , decreased the expression of
interleukin-1beta ,
tumor necrosis factor - alpha , and
metalloproteinase - 9 , and
squamous metaplasia features such as hyperproliferation and abnormal epidermal differentiation of conjunctival epithelium . -
pubmed
Statistical analyses were performed using Plink and R. RESULTS :
VKORC1 and
CYP2C9 were the major genetic determinants of
warfarin MWD and
SMWD , with
CYP4F2 having a smaller effect . -
pubmed
Genotypes for
VKORC1 and
CYP2C9 * 3 , age , height and weight , as well as other clinical factors such as
alcohol consumption , loading dose and concomitant drugs were important for the initial INR response to
warfarin . -
pubmed
VKORC1 and
CYP2C9 genetic polymorphisms are the most important determinants of
warfarin dosing , and it is highly unlikely that other common variants of clinical importance influencing
warfarin dosage will be found . -
pubmed
Both
VKORC1 and
CYP2C9 * 3 are important determinants of the initial INR response to
warfarin . -
pubmed
Cell blood count was assessed by sysmex system , serum
iron and total
iron binding capacity was measured by standard laboratory kit and for
ferritin ELISA kit was utilized . -
pubmed
NOX5 protein , one of the most active generators of reactive
oxygen species ( ROS ) , plays an important role in many processes , including regulation of cell growth ,
death and differentiation . -
pubmed
Contrary to other members of
NADPH - oxidases family ,
NOX5 has its own regulatory
calcium - binding domain and thus could be activated directly by
calcium ions . -
pubmed
In the present study using calorimetric methods and circular dichroism we found that oxidation of
cysteine and
methionine residues of
NOX5 decreases binding of
Ca2 + ions and perturbs both secondary and tertiary structure of protein . -
pubmed
Our data strongly suggest that oxidation of
calcium - binding domain of
NOX5 could be implicated in its inactivation , serving as a possible defense mechanism against oxidative stress . -
pubmed
Phosphatidic Acid Produced by
RalA - activated
PLD2 Stimulates Caveolae - mediated Endocytosis and Trafficking in Endothelial Cells . -
pubmed
PLD2 was shown to be activated by
RalA , and inhibition of
PLD2 abolished
Alexa-488 - BSA uptake , indicating that
phosphatidic acid (
PA ) generated by
PLD2 may facilitate caveolae - mediated endocytosis . -
pubmed
Thus , our results suggest that the small GTPase
RalA plays an important role in promoting invagination and trafficking of caveolae , not by potentiating the association between
Cav-1 and
FilA but by stimulating
PLD2 - mediated generation of
phosphatidic acid . -
pubmed
Nodulation , nodule development and senescence directly affects
nitrogen fixation efficiency , and previous studies have shown that inhibition of some
cysteine proteases delay nodule senescence , so their nature inhibitors ,
cystatin genes , are very important in nodulation , nodule development , and senescence . -
pubmed
On day three after seeding , the most apparent
vinculin - containing focal adhesion plaques were also found on samples with higher concentrations of
chromium . -
pubmed
On the other hand , the expression of
type I collagen and
alkaline phosphatase at the mRNA and protein level was the highest on
Cr - DLC samples with a lower concentration of
Cr ( 0 - 1.8 at.% ) . -
pubmed
The effects were assessed by comparing control and impact sites ( i ) on the composition of bacterial communities ; ( ii ) on the abundance of twelve antibiotic resistance genes ( ARGs ) encoding resistance to
beta - lactams ,
fluoroquinolones ,
sulphonamides ,
tetracyclines ,
macrolides and
vancomycin , as well as the class 1 integron -
integrase gene (
intI1 ) ; ( iii ) on the occurrence of wastewater - associated bacteria , including putative pathogens , and their potential linkage to target ARGs . -
pubmed
In this study , a DNA sample ( at 1 ng DNA target input in triplicate ) was spiked with five concentrations of five inhibitors (
humic acid ,
melanin ,
hematin ,
collagen , and
calcium ) and sequenced with both the HID - Ion AmpliSeq Library Kit and ID panel on the Ion PGM System and the ForenSeq DNA Signature Prep Kit on the MiSeq FGx . -
pubmed
The HID - Ion AmpliSeq Library Kit and ID panel and the ForenSeq DNA Signature Prep Kit both were susceptible to
humic acid , melanin , and
collagen ; however , the ForenSeq kit showed greater inhibition to melanin and
collagen than the AmpliSeq kit . -
pubmed
Because of reduced CF
ASL volumes , CF mucus concentrations increased and produced a greater dependence of
ASL volume regulation on cilia - mucus -
ATP release interactions in CF than NL epithelial nodules . -
pubmed
Similarly , the CF model was less tolerant to evaporation induced
ASL volume reduction at all
ATP release rates than the NL model . -
pubmed
Previous studies indicate that genes
GGT1 and
HNF1A may contribute to the abnormal
glucose metabolism and altered lipid profile that are important clinical features of
PCOS . -
pubmed
Our main outcome measures were the prevalence of major metabolic complications of
acromegaly (
diabetes ,
hypertension ,
low - density lipoprotein cholesterol ,
triglycerides ) at diagnosis and last follow - up in comparison with French epidemiological data . -
pubmed
Unlike
FTR ,
FDR contains a
rubredoxin [ 1Fe-0S ] center postulated to mediate electron transfer from
ferredoxin to the active - site [
4Fe-4S ] cluster . -
pubmed
UV - visible ,
EPR , and Mossbauer spectroscopic data indicated that two - electron reduction of the active - site
disulfide in
FDR involves a one - electron - reduced [
4Fe-4S]1 + intermediate previously hypothesized for
FTR . -
pubmed
Our results support a role for an active - site
tyrosine in FDR that occupies the equivalent position of an essential
histidine in the active site of
FTR . -
pubmed
At baseline and 4 h , middle cerebral artery blood flow velocity ( MCAv ) and
CO2 reactivity ( CVR ) of the MCA and carotid artery were measured using transcranial Doppler (
TCD ) and duplex ultrasound , respectively . -
pubmed
The groups were organized as Group I ( control ) , Group
II (
malathion ) and Group III (
malathion + lipid emulsion treatment ) . -
pubmed
RESULTS : A decrease of
PAS positive staining cells , and an increase of liver enzymes , formation of degenerative changes and apoptotic cell
deaths occurred in the
malathion group . -
pubmed
Within this context , high - rate activated sludge (
HRAS ) is the most promising alternative technology to redirect
carbon ( organic compounds ) towards energy as biogas . -
pubmed
However , the protective effects of
Salvianic acid A and Danshen granule on
Tat - induced
neurotoxicity remain unknown . -
pubmed
Here , we found that
Salvianic acid A and Danshen granule remarkable inhibited
Tat - induced cell
death , blocked
LDH release and rescued dendritic spine loss . -
pubmed
Furthermore ,
Salvianic acid A and Danshen granule significantly ameliorates
Tat - induced intracellular ROS and
MDA production , attenuates cell apoptosis . -
pubmed
In addition ,
Salvianic acid A and Danshen granule pretreatment obviously increases antioxidant enzymatic activity of
CAT ,
SOD and
GSH - Px and inhibits apoptotic pathways . -
pubmed
In conclusion , this study demonstrated that
Salvianic acid A and Danshen granule provides substantial neuroprotection against
Tat - induced
neurotoxicity , which may be new therapeutic agent in
Tat induced
HAND or
neurodegenerative diseases . -
pubmed
Fisetin decreased the expression of multiple signaling proteins (
beta - catenin ,
NF - kappaB ,
EGFR ,
STAT-3 ) acting upstream to
EMT and known to be involved in induction and maintenance of mesenchymal phenotype , which may explain the observed effects . -
pubmed
Overall , our study suggested the ability of
fisetin to serve as a potential therapeutic agent on its capacity to attenuate the
EMT program and inhibit migration , invasion and stem cell phenotype of
lung cancer cells . -
pubmed
To date , researchers have found that some
chloroplast proteins have
Ca2 + -binding sites , and the structure and function of some of these proteins have been discussed in detail . -
pubmed
We have shown a role for
ARNT /
HIF1beta in
glucose sensing and
insulin secretion in vitro and no defects in in vivo
glucose homeostasis . -
pubmed
The effects of the
HF diet rescued
insulin secretion in fl / fl / Cre islets could be reproduced by treating low fat diet fed fl / fl / Cre islets with the lipid signalling molecule
1 - monoacylglcyerol . -
pubmed
This suggests that the defects seen in low fat diet fed fl / fl / Cre islet
insulin secretion involves lipid signalling
molecules . -
pubmed
A multifunctional nanoparticle was simply synthesized by mixing a
TICT + AIE featured molecule (
TPAPP - CHO ) with
PBS solution . -
pubmed
[
IgA vasculitis with massive
hemorrhage from the jejunum after
steroid administration ] . -
pubmed
Importantly , the diameters and surface charges of the FNPs changed dramatically after 2 h of
post - treatment using 0.4 M
hydrogen peroxide (
H2O2 ) as the oxidant , indicating that the FNPs were highly ROS - sensitive . -
pubmed
In this Viewpoint , we review the state - of - the art of PET imaging with radiotracers that target the
GABAA - benzodiazepine receptor complex , challenges and opportunities for imaging
GABAB receptors and
GABA transporters , and highlight an ongoing need to develop more sensitive radiotracers for imaging
GABA release in the central nervous system . -
pubmed
After 4 weeks of treatment ,
cinacalcet significantly decreased the
oxidized albumin ratio which is a ratio of reduced and oxidized forms of
HSA via increasing reduced form of
HSA . -
pubmed
In conclusion ,
cinacalcet improves the redox status of
HSA by inhibiting
PTH production and partially by its radical scavenging action . -
pubmed
Overall , this study demonstrates the effectiveness of 3D printed
tacrolimus suppositories to ameliorate
colitis and highlights the utility of non - invasive PET /
CT imaging to evaluate new therapies in the preclinical area . -
pubmed
The proportion of statins ,
aspirin ,
clopidogrel ,
angiotensin - converting enzyme inhibitors /
angiotensin II receptor blockers ( ACEI / ARB ) , and beta - blocker used at admission was 88.8 % , 86.9 % , 84.6 % , 51.7 % , and 61.4 % , respectively . -
pubmed
fermentum 111 , to remove
aflatoxin M1 (
AFM1 ) from a
phosphate buffer solution (
PBS ; spiked with 0.15 microg / mL
AFM1 ) . -
pubmed
Levels of
AFM1 in
PBS were measured with high - performance liquid chromatography . -
pubmed
Viable and non - viable cells of all examined isolates were capable of removing
AFM1 in
PBS with removal percentage values in the range of 73.9 - 80.0 % and 72.9 - 78.7 % , respectively . -
pubmed
Since the historical use of
gonadotrophin and
estradiol levels to define the different
anovulatory disorders has shown some limitations , the use of other markers such as anti - mullerian hormone (
AMH ) has been proposed . -
pubmed
Effects of
Sr2 + and
Mg2 + on the
phospholipase A and the presynaptic neuromuscular blocking actions of beta - bungarotoxin , crotoxin and taipoxin . -
pubmed
The antitumor effects in vivo of
rhIL-6 appear to be mediated indirectly as
rhIL-6 had no effect on proliferation of LLC cells in vitro as assessed by colony and
3H - thymidine incorporation assays . -
pubmed
Phorbol myristate acetate (
PMA ; 20 - 100 ng / ml ) triggered only
acetyltransferase activation , without concomitant lyso - paf (
1 - O - alkyl - sn - glycero-3 - phosphocholine ) and paf formation . -
pubmed
A low concentration of
PMA ( 5 ng / ml ) potentiated antigen - induced degranulation ,
acetyltransferase activation and paf formation by about 30 % but did not change the level of lyso - paf formation . -
pubmed
Overnight treatment of mast cells with
PMA ( 200 ng / ml ) caused disappearance of
protein kinase C (
PKC ) from both cytosol and membranes . -
pubmed
The
PKC inhibitors
chlorpromazine and
staurosporine inhibited to a significant extent both cell degranulation and all steps leading to paf biosynthesis . -
pubmed
The
PKC - dependent signal directly generated by
PMA or
diacylglycerol is not sufficient to trigger the full cell response , which is obtained only through receptor - operated antigen challenge . -
pubmed
Inhibition of
cyclo - oxygenase with
indomethacin ,
aspirin or
meclofenamate abolished the vasoconstrictor effect of
bradykinin , showing that a member of the
arachidonic acid cascade may be involved . -
pubmed
The
endoperoxide H2 / thromboxane A2 receptor antagonist
SQ 29548 significantly reduced the vasoconstrictor effect of
bradykinin , but did not affect the vasoconstrictor response to
noradrenaline ,
adrenaline ,
vasopressin ,
5 - hydroxytryptamine or
prostaglandins . -
pubmed
We isolated and characterized a
rat neutrophil defensin ,
RatNP-2 , that differs from the previously described
defensin RatNP-1 by containing
Ser-7 in place of
Arg-7 . -
pubmed
A study was made of the number of pulmonary endocrine cells , immunoreactive for
gastrin - releasing peptide (
bombesin ) or
calcitonin , in the terminal bronchioles of 39 cases of
pulmonary vascular disease . -
pubmed
Proliferating cells arrested at the
G1 - S boundary by exposure to
cytosine arabinoside showed an increased
PCNA immunofluorescence as compared to unstimulated cells . -
pubmed
However , the physiological phase consists of the activation of a
KCl +
NaCl carrier , while the
Na + in turn is pumped out by
Na +- K + ATPase , with a resultant net accumulation of
KCl . -
pubmed
,
antigens seen in the context of
self - class I molecules . -
pubmed
[
Triglyceride and
low - density lipoprotein cholesterol (
LDL - C ) levels in a population of men aged 35 - 54 years from 8 industrial plants in Wroclaw ] . -
pubmed
Heptachlor and
chlordane also caused only partial inhibition of oxidation , but were more toxic with cell - free extract
nitrite oxidase . -
pubmed
Differential response patterns to
MAO inhibitors and
tricyclics . -
pubmed
We found a linear negative correlation ( P less than 0.001 ) between the amount of
LDL cholesterol in the incubation solution and
prostaglandin I2 synthetase activity , whereas there was a positive correlation ( P less than 0.01 ) between
HDL cholesterol and
prostaglandin I2 synthesis . -
pubmed
A very low concentration of
LDL cholesterol and a high concentration of HDL
cholesterol stimulated
prostaglandin I2 synthesis , whereas a high
LDL cholesterol concentration inhibited
prostaglandin I2 biosynthesis by 64 % . -
pubmed
The concentration range of
LDL and
HDL cholesterol was representative of physiologically low , normal or elevated levels of lipoproteins . -
pubmed
The examinations included measurements of erythrocyte count , hematocrit , mean corpuscular volume ( MCV ) , blood and urine lead concentrations , urine
coproporphyrin and
delta - aminolevulinic acid , membrane
cholesterol (
CHO ) and phospholipids (
PL ) , total and free
CHO in plasma , and
lecithin cholesterol acyltransferase (
LCAT ) activity in plasma . -
pubmed
But no significant difference was observed in total
CHO in plasma , erythrocyte count , hematocrit , MCV , and
LCAT activity between both groups . -
pubmed
We proved that ( 1 ) elevated pulmonary arteriolar resistance (
PAR ) is not related to pulmonary blood flow and volume , pleural pressure , arterial
oxygen or
carbon dioxide tension and pH , left ventricular filling pressure and function ; ( 2 ) systemic vascular resistance ( SVR ) significantly correlates with
PAR ; ( 3 )
calcium - channel blockade with
nifedipine reduces systemic and pulmonary arterial pressures toward normal and significantly lowers both SVR and
PAR ; ( 4 ) the percent decrease in vascular resistance after
nifedipine is related to the baseline level of resistance in both the greater and the lesser circulations . -
pubmed
Formic acid pretreatment markedly enhanced the reactivity to
anti - IAPP8 - 17 antibody ; however , it failed to show the reactivity to anti - N - terminal and C - terminal flanking peptide antibodies . -
pubmed
Formic acid pretreatment of pancreatic tissue sections prepared for immunostaining is useful for visualization of buried epitopes of mature
IAPP and its precursor molecules , either in islet
amyloid deposits or in the islet cells . -
pubmed
At high loadings the specific activity of immobilized whole
antibody was lower for the large antigen than for the small
hapten , whereas no effect of hapten size on n was evident for either immobilized
Fab ' or
Fv fragments . -
pubmed
There was a significant reduction in serum
insulin levels and
glucose clearance rates without overt
diabetes for 30 Gy and 35 Gy ( p & lt ; 0.05 ) . -
pubmed
The concentrations of
VPA in plasma ( Css for total and C(uss ) for unbound drug ) and various tissues (
CT ) were determined . -
pubmed
Nuclease stability as dominant factor in the antiviral activity of
oligonucleotides directed against
HSV-1 IE110 . -
pubmed
A minimal protection strategy , consisting of end - capping and
pyrimidine protection , has proven to be particularly useful , because it not only yields
nuclease - resistant
oligonucleotides but also minimizes non - sequence - specific effects . -
pubmed
However , there was loss of circadian variation in serum
cortisol concentrations and administration of high - dose oral
dexamethasone was followed by a marked and reproducible rise in both
ACTH and
cortisol . -
pubmed
A good histologic response , an
LDH baseline value within the normal range , and the use of high - dose
MTX were positive predictive factors for DFS . -
pubmed
CONCLUSIONS : In non - metastatic
osteosarcoma of the extremity , chemotherapy - induced
tumor necrosis , the baseline
LDH serum value and the use of
HDMTX are significant predictive factors for DFS . -
pubmed
Adaptation of
gamma - aminobutyric acid type A receptors to
alcohol exposure : studies with stably transfected cells . -
pubmed
We studied the adaptation of
gamma - aminobutyric acid type A ( GABAA ) receptor function to chronic
ethanol exposure in cells stably transfected with the following
GABAA receptor subunits :
alpha-1 beta-2 gamma-2L ,
alpha-1 beta-2 gamma-2S ,
alpha-1 beta-3 gamma-2S ,
alpha-1 beta-1 , alpha-5 beta-3 gamma-3 and
alpha-6 beta-3 gamma-2S. -
pubmed
Direct activation of the
GABAA receptor by
pentobarbital was enhanced by chronic
ethanol treatment . -
pubmed
Chronic
ethanol exposure produced a reduction in the Emax value for the action of
muscimol for all six subunit combinations , but quantification of
surface receptors by immunolabeling showed no change in
GABAA receptor density . -
pubmed
The differences in
alcohol sensitivity and time courses for different effects of
ethanol indicate multiple mechanisms of adaptation of
GABAA receptors . -
pubmed
Since
glutamate reuptake is the primary mechanism for controlling extracellular
glutamate levels , the present study was conducted to examine whether chronic neuroleptic exposure alters gene expression for the
glutamate transporter
GLT-1 in the striatum . -
pubmed
Although both
haloperidol and
clozapine treatment for 30 days significantly decreased
GLT-1 expression from normal , the effects of
haloperidol treatment were consistently , and in the dorsal striatum , significantly greater than those of
clozapine . -
pubmed
When unfolded proteins accumulate in the endoplasmic reticulum (
ER ) , transcription of
glucose - regulated proteins (
GRPs ) representing
ER - resident molecular chaperones is markedly induced via the unfolded protein response ( UPR ) pathway . -
pubmed
In a fourth ,
iron gluconate ( 62.5 mg i.v./HD ) increased
transferrin saturation to 27 % and reduced the percentage of hypochromic red cells from 12 to 4 , while reticulocyte haemglobin remained & gt ; 30 pg . -
pubmed
Transposon mini - Tn10 insertional mutagenesis was applied to strain 406 , which revealed that
lpa-8 (
sfp ) ( encoding
4 '- phosphopantetheinyl transferase ) and the
pps operon ( located between 167 and 171 degrees ) are essential for
plipastatin production . -
pubmed
By using a new high - performance liquid chromatography system , we revealed that strain 168 expressing only
lpa-8 can also produce
plipastatin , although the yield is very low . -
pubmed
However , the introduction of the pleiotropic regulator
degQ of strain YB8 into
strain 168 expressing
lpa-8 resulted in a 10 - fold increase in the production of
plipastatin . -
pubmed
Air entrainments on
charcoal filters , extracted with
CS ( 2 ) and subsequently with
acetone , were analyzed by coupled gas chromatography ( GC)-electroantennogram (
EAG ) as well as by
GC - mass spectrometry . -
pubmed
The CS(2 ) extract contained 12
EAG - active peaks from which
benzaldehyde ,
octanal ,
limonene ,
1,8 - cineol ,
methyl benzoate ,
nonanal ,
decanal and
geranyl acetone were identified . -
pubmed
In the
acetone extract we identified eight
EAG - active peaks as
phenol ,
nonanal ,
2 - ethylhexanoic acid ,
octanoic acid ,
benzoic acid ,
nonanoic acid ,
decanoic acid and
dimethyl phthalate . -
pubmed
Two acidic
amino acid residues ,
Asp(470 ) and
Glu(471 ) , contained in the
carboxyl cytoplasmic tail of a major lysosomal membrane protein ,
LGP85 /
LIMP II , are important for its accumulation in secondary lysosomes . -
pubmed
Lysosomal membrane glycoprotein termed
LGP85 or
LIMP II has a
COOH - terminal cytoplasmic tail whose
amino acid sequence is R(459)GQGSMDEGTADERAPLIRT(478 ) . -
pubmed
Two acidic
amino acid residues , D(470 ) and E(471 ) , in the cytoplasmic tail of
LGP85 are crucial for its binding to adaptor - like complex
AP-3 . -
pubmed
In the present study we investigated their role(s ) in intracellular distributions of
LGP85 using two
alanine substitution mutants at D(470 ) and E(471 ) ( defined as D470A and E471A , respectively ) . -
pubmed
Plasma
fatty acids , blood lipids , and
apolipoproteins were analyzed at 15 months , and dietary intakes at 12 , 15 , and 18 months . -
pubmed
In vivo , the
TPGS - incorporated film exhibited a two - fold increase in
FA when compared to the control film containing the
PEG 3350 5 % . -
pubmed
The carbomer 971P and
polycarbophil containing films were determined to have the highest
FA and EAF , and the lowest MA of all films tested . -
pubmed
ChAT activity was increased by
retinoic acid ,
dexamethasone , and
dibutyrylcyclic AMP (
dbcAMP ) , and a synergistic effect was observed with a combined
dexamethasone and
dbcAMP treatment . -
pubmed
Hemicholinium-3 - sensitive
choline uptake ,
hemicholinium-3 binding , and
acetylcholine content were increased by differentiating agents , with a rank order of potency comparable to their effects on
ChAT activity . -
pubmed
Some of the
carbohydrate components detected by
ELLAs as well as two organic matrix proteins (
SM30 and
SM50 ) were localized within different subregions of the spine skeleton using field - emission scanning electron microscopy . -
pubmed
Lysine and
polyamines are substrates for transglutamination of
Rho by the Bordetella dermonecrotic toxin . -
pubmed
Bordetella dermonecrotic toxin (
DNT ) catalyzes the transglutamination of
glutamine-63/61 of
Rho GTPases , thereby constitutively activating
Rho proteins . -
pubmed
The enzymatically active fragment of
DNT ( residues 1136 to 1451 , DeltaDNT ) induced the incorporation of
L -[( 14 ) C ] lysine in
RhoA in a concentration - dependent manner . -
pubmed
Also ,
Rac and
Cdc42 , but not
Ras , were transglutaminated with
lysine by
DeltaDNT . -
pubmed
Transglutamination of the
GTPase with
L - lysine inhibited intrinsic and
Rho - GAP - stimulated
GTP hydrolysis of
RhoA. -
pubmed
In contrast to
lysine , treatment of
RhoA with
alanine ,
arginine , and
glutamine were not able to substitute for
lysine in the transglutamination reaction . -
pubmed
A comparison of
putrescine ,
spermidine , and
lysine as substrates for
DNT - induced transglutamination of
RhoA revealed that
lysine is a preferred second substrate at least in vitro . -
pubmed
Time - segmented
MS2 scans enabled simultaneous monitoring of
Met - enkephalin ,
Leu - enkephalin , and unknown peptides . -
pubmed
Blood
glucose monitoring is recommended : to evaluate the glycemic response to the effect of
insulin ; to confirm
hypoglycemia ; to prevent
hyperglycemia in case of disease ; in case of physical exercise . -
pubmed
Flavopiridol , a
cyclin - dependent kinase inhibitor , enhances radiosensitivity of
ovarian carcinoma cells . -
pubmed
Flavopiridol , a
cyclin - dependent kinase (
cdk ) inhibitor , can cause cell cycle arrest , induce apoptosis in
cancer cells , and inhibit
tumor cell growth in vivo . -
pubmed
In addition ,
flavopiridol treatment ( 300 nM , 1 day ) resulted in decreased levels of
Ku70 and
Ku86 proteins that play a role in DNA repair processes , suggesting that DNA repair processes may have been disrupted by this agent . -
pubmed
Flavopiridol down - regulated
cyclin D1 and
cyclin E protein levels and also inhibited phosphorylation of
retinoblastoma protein , which is inconsistent with the observed cell cycle arrest . -
pubmed
Among the
cdks tested ,
cdk-9 , the catalytic subunit of
positive transcription elongation factor b , was significantly down - regulated by
flavopiridol , suggesting that
flavopiridol may modulate cellular transcription processes . -
pubmed
The objective of this study was to investigate the use of
folate - targeted liposomes for the delivery of encapsulated
oligonucleotides to
folate receptor (
FR)-positive
tumor cells in vitro and in vivo . -
pubmed
Importantly , cell association was blocked by a large excess
folic acid , thus reflecting the
FR - specific nature of the cell interaction . -
pubmed
We conclude from these results that
folate liposomes can effectively deliver oligonucleotides into
folate receptor - bearing cells in vitro , but additional barriers exist in vivo that prevent or decrease effective
tumor uptake and retention . -
pubmed
Ca ( 2 + ) sensitivity of arterial contractility is governed by regulating
myosin phosphatase activity in response to agonist stimuli . -
pubmed
Agonists , such as an alpha(1)-agonist and
endothelin-1 , produced significant contraction in arteries of all species under physiological
Ca ( 2 +)-containing conditions . -
pubmed
Thus ,
CPI-17 is essential for
G protein /
PKC - mediated
Ca ( 2 + ) sensitization in smooth muscle . -
pubmed
The ability of
Fe chelation by 100 microM
SIH to limit enlargement to approximately 180 % confirms that the
LIP plays a major role in
cytomegaly . -
pubmed
Pretreatment of the cells with
PACAP resulted in a significant decrease in LPS- or
IFNgamma - induced
NO production as well as
iNOS and IL-1beta mRNA levels . -
pubmed
The inhibitory effect of
PACAP appeared to be mediated through an increase in intracellular
cAMP . -
pubmed
PACAP inhibition of LPS - induced
NO production was accompanied by inhibition of
p38 MAPK activation , but not
ERK ,
JNK , or
NF - kappaB. -
pubmed
Topotecan - induced alterations in the amount and stability of
human DNA topoisomerase I in solid
tumor cell lines . -
pubmed
MATERIALS AND METHODS : Western blot analysis of the time - dependent redistribution of a full - size
topo 1 and its proteolytical fragments was performed after
Tpt treatment for 1 h at concentrations 10 - fold or 100 - fold higher than the Tpt IC50 for the respective cell lines . -
pubmed
CONCLUSION : The data obtained indicate that
Tpt - induced time- and dose - dependent effects on the amount and stability of
topo 1 are involved in the mechanisms of
Tpt activity against different solid
tumor cell lines . -
pubmed
This is in sharp contrast to the importance of this type of proteins , particularly
G protein - coupled receptors (
GPCRs ) , as
today 60 - 70 % of the current drug targets are based on membrane proteins . -
pubmed
ATP hydrolysis in the presence and absence of
ouabain was used to measure
Na , K - ATPase activity . -
pubmed
Western blot analysis was used to examine
phosphotyrosine - containing proteins and
tyrosine kinase expression . -
pubmed
Na , K - ATPase activity in membrane material treated with
Lck or
Fes was not significantly altered , even though
Lck and
Fes treatment induced robust
tyrosine phosphorylation . -
pubmed
Added exogenously , each
tyrosine kinase induced a different pattern of membrane protein
tyrosine phosphorylation . -
pubmed
As judged by immunoprecipitation ,
Src ,
Fyn ,
Lyn , and
Lck elicited
tyrosine phosphorylation of the
Na , K - ATPase alpha1 protein . -
pubmed
This could perhaps explain why inhibition of
Na , K - ATPase activity is reported to be caused by
tyrosine phosphorylation in some tissues , whereas stimulation of
Na , K - ATPase activity is observed in other tissues . -
pubmed
We investigated whether
H2O2 ,
superoxide , and
ERK participate in
nerve growth factor (
NGF)-induced signaling cascades and whether antioxidant
N - acetylcysteine (
NAC ) regulates these
NGF - induced responses . -
pubmed
We found that
NGF increased intracellular
H2O2 concentration and activated
ERK but failed to affect intracellular
superoxide level . -
pubmed
Moreover ,
NAC counteracted these
NGF - induced responses . -
pubmed
The variability in
CsA and
Tac disposition has been attributed to interindividual differences in the expression of the metabolizing enzymes
cytochrome P450 (
CYP )
3A4 and
3A5 , and in the expression of the
drug transporter P - glycoprotein ( encoded by the
ABCB1 gene , formerly known as the multidrug resistance 1 gene ) . -
pubmed
In addition , we determined if
DCEs could serve as surrogate inducers of
BMO gene expression . -
pubmed
The impacts of
lactic acid - supported
DCE oxidation on
BMO activity differed among the isomers . -
pubmed
In intact cells , 50 % of
BMO activity was irreversibly lost after consumption of approximately 20 nmol mg
protein(-1 ) of
1,1 - DCE and
1,2 - trans DCE in 0.5 and 5 min , respectively . -
pubmed
In contrast , a comparable loss of activity required the oxidation of 120 nmol
1,2 - cis DCE mg
protein(-1 ) . -
pubmed
butanovora " did not express
BMO activity but gained activity after exposure to
butane ,
ethene ,
1,2 - cis DCE , or
1,2 - trans DCE . -
pubmed
The products of BMO activity ,
ethene oxide and
1 - butanol , induced
lacZ in a reporter
strain containing
lacZ fused to the BMO promoter , whereas
butane ,
ethene , and
1,2 - cis DCE did not . -
pubmed
1,2 - trans DCE was unique among the
BMO substrates tested in its ability to induce
lacZ expression . -
pubmed
Pre - incubation with 50 microM
3 - quinuclidinyl benzilate (
QNB ) or 50 nM
strychnine failed to inhibit the
ACh - initiated
AR , demonstrating that
muscarinic AChRs and
nAChRs containing alpha9 subunits do not mediate this event . -
pubmed
Choline ( 2.5 , 5 and 10 mM ) , a highly specific but low potency agonist of the
alpha7 nAChR initiated
AR , with its effect blocked by the
nAChR antagonist
methyllycaconitine (
MLA ) . -
pubmed
The
nAChR antagonists , alpha - bungarotoxin (
alpha - BTX ) and
MLA , reduced the
ACh - initiated
Ca2 + rise by 75 and 78 % , respectively , demonstrating the majority of the rise is mediated through
nAChRs containing
alpha7 or alpha9 subunits . -
pubmed
The remaining sperm ( 6 % ) displayed a sharp spike of
Ca2 + ( approximately 1 min ; 86 % + /- 4.3 SEM change in fluorescence intensity ) , followed by abrupt loss of fluorescence , a pattern suggestive of
AR . -
pubmed
Indeed , there is growing evidence that the
gonadotropin ,
luteinizing hormone , which regulates serum
estrogen and
testosterone concentrations , could be an important causative factor in the development of
Alzheimer ' s disease . -
pubmed
Inhibition of
indoleamine 2,3 dioxygenase activity by
H2O2 . -
pubmed
Indoleamine 2,3 - dioxygenase is the first and rate limiting enzyme of the
kynurenine pathway of
tryptophan metabolism , has potent effects on cell proliferation and mediates antimicrobial , antitumorogenic , and immunosuppressive effects . -
pubmed
The work presented here represents the first systematic study exploring the mechanisms by which low levels of
hydrogen peroxide ( 10 - 100 microM ) inhibit
indoleamine 2,3 - dioxygenase in vitro . -
pubmed
Following brief
peroxide exposure both
enzyme inhibition and structural changes were observed . -
pubmed
Enzyme activity could in part be preserved in the presence of
sulfhydryl containing compounds , particularly
DTT and
methionine . -
pubmed
Some
enzyme activity could be recovered in the presence of
thioredoxin , indicating that the inhibitory effect of
H ( 2 ) O ( 2 ) is at least partially reversible in vitro . -
pubmed
We present evidence that
cysteine oxidation represents one mechanism of
indoleamine 2,3 - dioxygenase inhibition . -
pubmed
Cessation of short - term
alcohol administration thus had no promoting effects on development of
GST - P foci , suggesting that the duration of
alcohol treatment may be important . -
pubmed
Our hypothesis that plant annexins mediate exocytotic events is supported by the finding that
p23 ,
p33 , and
p35 bind to these secretory vesicles in a
Ca ( 2 +)-dependent manner . -
pubmed
Homochiral
1D zinc - quitenine coordination polymer with a high dielectric constant . -
pubmed
We further demonstrated that
His384 , a universally conserved residue among
protein tyrosine kinases , is not essential for
Src catalysis or its inactivation by C - terminal tail
Tyr phosphorylation . -
pubmed
Interestingly ,
His384 mutants undergo autophosphorylation on
Tyr416 like wild - type
Src but are not activated by autophosphorylation . -
pubmed
The potential role of
His384 in
Src activation by autophosphorylation is discussed in the context of
Src structure . -
pubmed
Increased cell survival and decreased
chlorophyll degradation in transformants under acclimated conditions further confirmed the relevance of
codA in imparting stress tolerance . -
pubmed
Aer residues 210 to 290 were individually replaced with
cysteine and then cross - linked in vivo . -
pubmed
Several findings supported the hypothesis that
Aer has a four - helix
HAMP structure : ( i ) cross - linking independently identified the same residues at the dimer interface that were predicted by the model , ( ii ) the rate of cross - linking for residue pairs was inversely proportional to the beta -
carbon distances measured on the model , and ( iii ) clockwise
lesions that were not contiguous in the linear Aer sequence were clustered in one region in the folded
HAMP model , defining a potential site of PAS -
HAMP interaction during signaling . -
pubmed
posadasii identification was confirmed by nested PCR of the antigen 2 /
proline - rich
antigen (
Ag2 /
PRA ) encoding gene . -
pubmed
An
argatroban infusion was started at 2 microg / kg / minute during her 33rd week of pregnancy , with the dosage titrated based on the activated partial
thromboplastin time ; infusion rates ranged from 2 - 8 microg / kg / minute . -
pubmed
Periodic acid - Schif staining and immunohistochemical staining of
CD34 were conducted to explore if
VM exists in those Rb specimens . -
pubmed
OBJECTIVE : The antiprogestin
mifepristone is widely used for medical termination of pregnancy (
TOP ) . -
pubmed
Medical
TOP was induced with 200 mg of
mifepristone followed by 0.8 mg of
misoprostol 2 days later . -
pubmed
Three - dimensional ultrasound examinations and hormone assays (
progesterone ,
human chorionic gonadotropin , and
17 - hydroxyprogesterone ) were performed in both groups on the day of , and 2 days after , administration of
mifepristone . -
pubmed
The esterification of
cholesterol in plasma by
LCAT was 67 % and in tissues by
ACAT was 64 % . -
pubmed
The relative esterification (
cholesterol being 1.0 ) of these
sterols by the plasma
LCAT was 1.00 , 0.95 , 0.89 , 0.40 , 0.85 , 0.82 and 0.80 , 0.69 and 0.82 , respectively . -
pubmed
The predominant
fatty acid of the
sterol esters was
linoleic acid for
LCAT and
oleic acid for
ACAT . -
pubmed
The saturation of the A ring of
cholesterol increased
ester formation by
ACAT by 29 % and decreased the esterification by
LCAT by 5.9 % . -
pubmed
Esterification by
ACAT and
LCAT was reduced , respectively , by 25 and 11 % by the presence of an additional
methyl group on the side chain of
cholesterol at the C-24 position . -
pubmed
This data supports our hypothesis that the structure of the
sterol substrate has a significant effect on its esterification by
ACAT or
LCAT . -
pubmed
Alternatively , we show through two - color spectro - electrochemistry that Raman scattering is also finely sensitive to oxidation state changes of
flavin , a biomimetic system that mimics the active center of many
flavoprotein enzymes . -
pubmed
CONCLUSIONS : Our study confirmed a close association between preoperative
glucose control indicated by
HbA ( 1c ) levels & lt;7 % and a decreased risk of postoperative
infectious complications . -
pubmed
In critical patients sources of oxidative stress include the mitochondrial respiratory electron transport chain ,
xanthine oxidase activation , the respiratory burst associated with neutrophil activation , and
arachidonic acid metabolism . -
pubmed
Several endogenous antioxidants have been identified including enzymes , like
superoxide dismutases and
glutathione peroxidase , vitamins and other molecules such as
uric acid and
bilirubin . -
pubmed
We present a case of metastatic
CDC that responded favorably to a multiple
tyrosine kinase inhibitor ,
sunitinib , achieving a partial response in both lung and skeletal
metastases . -
pubmed
Considering these findings , it would be worthwhile prospectively investigating the role of multiple
tyrosine kinase inhibitors , particularly
sunitinib , in the management of metastatic
CDC . -
pubmed
Muscarinic acetylcholine receptors in the nucleus accumbens core and shell contribute to
cocaine priming - induced reinstatement of drug seeking . -
pubmed
Muscarinic acetylcholine receptors in the nucleus accumbens play an important role in mediating the reinforcing effects of
cocaine . -
pubmed
However , there is a paucity of data regarding the role of accumbal
muscarinic acetylcholine receptors in the reinstatement of
cocaine - seeking behavior . -
pubmed
The goal of these experiments was to assess the role of
muscarinic acetylcholine receptors in the nucleus accumbens core and shell in
cocaine and
sucrose priming - induced reinstatement . -
pubmed
Results indicated that systemic administration of the
muscarinic acetylcholine receptor antagonist
scopolamine ( 5.0 mg / kg , i.p . ) -
pubmed
In contrast , infusion of
scopolamine ( 36.0 mug ) directly into the accumbens core , but not shell , attenuated
sucrose reinstatement , which suggests that
muscarinic acetylcholine receptors in these two subregions of the nucleus accumbens have differential roles in
sucrose seeking . -
pubmed
Taken together , these results indicate that
cocaine priming - induced reinstatement is mediated , in part , by increased signaling through
muscarinic acetylcholine receptors in the shell subregion of the nucleus accumbens . -
pubmed
The proposed model is successfully applied to estimate the site energy distribution of three pitch - based activated
carbons (
PA , PFeA and
PBA ) developed in our laboratory and also for other common adsorbent materials for different gas molecules . -
pubmed
We planned this case - control study to evaluate the relationship between
hypogonadism , as defined by the sexual hormone profile (
FSH ,
LH ,
testosterone ( T ) and
prolactin ) and ED , as assessed by means of an internationally validated self - administered questionnaire ( IIEF ) . -
pubmed
Mean
alcohol consumption significantly differed in the three
ALDH2 genotypes : 297 g per week in
NN , 158 g in
NM and 18 g in MM . -
pubmed
These results suggest an interactive effect of
ALDH2 and
P450 2E1 genes on
alcohol consumption and a higher sensitivity to
alcohol - induced health effects in c2/2 genotypes , although larger scale studies are required to confirm these findings . -
pubmed
Frataxin homologues are important
iron chaperones in eukarya and prokarya . -
pubmed
In a four - constituents reconstitution system ( including
SufU ,
SufS ,
Fra and
CitB ) we observed a
Fra - dependent formation of a
[ 4 Fe-4 S ] cluster on
SufU that could be efficiently transferred onto the target
apo - aconitase (
CitB ) . -
pubmed
Purine /
Pyrimidine nucleotides ratio ( OD 260 : OD 280 ) was also measured RESULTS :
P5'N-1 deficient patients showed reduction in
P5'N-1 activity ( Mean + - SD ; 4.06 + - 0.66 using an ELISA reader & amp ; 6.25 + - 1.37 using a spectrophotometer ) as compared to the normal control group ( ELISA reader : 13.24 + - 3.42 & amp ; Spectrophotometer : 18.25 + - 3.20 ) . -
pubmed
Heterozygotes showed intermediate activity ( ELISA reader : 6.06 + - 0.48 & amp ; Spectrophotometer : 8.06 + - 1.28 ) , however they would have been missed on screening using the
Purine /
Pyrimidine nucleotides ratio CONCLUSION : Determination of
P5'N-1 activity by using an ELISA reader is a new , simple , less time consuming and reliable method . -
pubmed
MTS assay was used to measure the ability of DC transduced with
AAV -
AFP ( AAV -
AFP + DC ) to stimulate the proliferation of T cell . -
pubmed
For each SD increase in log
FABP4 , hazard ratios ( HRs ) for
diabetes were 1.35 ( 95 % CI 1.10 - 1.65 ) for women and 1.45 ( 1.13 - 1.85 ) for men controlling for age , race , education , physical activity ,
cystatin C ,
alcohol intake , smoking , self - reported health status , and
estrogen use for women ( P for sex -
FABP4 interaction 0.10 ) . -
pubmed
circinelloides is
beta - carotene , although it has some
hydroxylase activity and produces low amounts of
zeaxanthin . -
pubmed
Two enzymatic activities are required for the production of
astaxanthin from
beta - carotene : a
hydroxylase and a
ketolase . -
pubmed
Using site - directed mutagenesis and high - resolution
copper -
phenanthroline footprinting , we have identified the functional toxboxes required for the
ToxT activation of
PctxAB . -
pubmed
AIM : To assess the effect of simulated mouth conditions reproduced with thermocycling on the tensile bond strength of two
silicone based resilient denture liners with acrylic resin bases . -
pubmed
Serum
FSH ,
LH , and
estradiol (
E2 ) were measured . -
pubmed
In this study , we present a theory on how the
CO2 diffusion gradient over the gasket is affected by leaf - mediated pores (
LMP ) and how
LMP reduce diffusive exchange across the gaskets . -
pubmed
However , a possible correlation between RBC
MTX and
MTXGlu2 - 5 concentrations and clinical outcomes of
MTX treatment in
RA is debated . -
pubmed
AIM : The purpose of this analysis was to describe the pharmacokinetics of
MTX and
MTXGlu ( 2 - 5 ) in RBCs . -
pubmed
Dexmedetomidine , a selective
alpha2 - adrenergic receptor agonist , is often used for sedation and analgesia especially in intensive care units . -
pubmed
Following this , local immune response was investigated by measuring
cytokine levels in the presence of
dexmedetomidine in spleen cell culture . -
pubmed
According to our in vitro and in vivo results ,
dexmedetomidine shows anti - inflammatory effect by increasing
IL-4 and
IL-10 levels responsible from anti - inflammatory response via Th2 pathway . -
pubmed
Moreover , we showed for the first time in the study that
dexmedetomidine administration reduces
IL-23 , which is responsible from initiation of inflammatory response via Th17 pathway . -
pubmed
, a facultative C3 halophyte species , can efficiently control the activity of slow ( SV ) and fast ( FV )
tonoplast channels to match specific growth conditions by ensuring that most of accumulated
Na + is safely locked in the vacuole ( Bonales - Alatorre et al . -
pubmed
The interaction between an adsorbed
1 - butyl-1 - methylpyrrolidinium bis ( trifluoromethylsulfonyl ) imide ,
[ BMP ][ TFSA ] , ionic liquid ( IL ) layer and a
Ag ( 111 ) substrate , under ultrahigh - vacuum conditions , was investigated in a combined experimental and theoretical approach , by high - resolution scanning tunneling microscopy (
STM ) , X - ray photoelectron spectroscopy ( XPS ) , and dispersion - corrected density functional theory calculations ( DFT - D ) . -
pubmed
DHEAS ,
testosterone , FTI ,
SHBG ,
estriol ,
progesterone ,
beta - hCG ,
TSH , free T3 and free T4 concentrations did not differ between the groups . -
pubmed
Pepsin in the gastric fluid would compete for the adsorption sites with the adsorbates , and
bile salts in the intestinal fluid had a solubilization effect on the chemicals . -
pubmed
However , as we reported herein ,
citric acid covalently modified a recombinant
monoclonal antibody (
IgG1 ) in a
phosphate /
citrate - buffered formulation at pH 5.2 and led to the formation of so - called " acidic species " that showed mass increases of 174 and 156 Da , respectively . -
pubmed
ST7612AA1 , a
thioacetate - omega ( gamma - lactam carboxamide ) derivative selected from a novel generation of oral
HDAC inhibitors . -
pubmed
A systematic study of medicinal chemistry aimed at identifying a new generation of
HDAC inhibitors , through the introduction of a
thiol zinc - binding group ( ZBG ) and of an
amide - lactam in the omega - position of the
polyethylene chain of the
vorinostat scaffold , allowed the selection of a new class of potent pan -
HDAC inhibitors ( pan - HDACis ) . -
pubmed
Molecular docking into
HDAC crystal structures suggested a binding mode for these
thiol derivatives consistent with the stereoselectivity observed upon insertion of
amide -
lactam substituents in the omega - position . -
pubmed
ST7612AA1 ( 117 ) , selected as a drug candidate for further development , showed an in vitro activity in the nanomolar range associated with a remarkable in vivo antitumor activity , highly competitive with the most potent
HDAC inhibitors , currently under clinical trials . -
pubmed
Greater PAV regression in women versus men occurred with
rosuvastatin ( p = 0.004 ) , those with
diabetes ( p = 0.01 ) , stable
coronary disease ( p = 0.01 ) , higher baseline
LDL - C ( p = 0.02 ) , and higher
CRP ( p = 0.04 ) levels . -
pubmed
Annexin A5 promotes invasion and chemoresistance to
temozolomide in
glioblastoma multiforme cells . -
pubmed
In the present study , we explored the effects of
annexin A5 on
GBM cell invasion and chemoresistance to
temozolomide . -
pubmed
Overexpression of
annexin A5 in both cell lines significantly increased cell invasion ,
matrix metalloproteinase-2 (
MMP-2 ) expression / activity ,
Akt phosphorylation at
serine 473 , and the half maximal inhibitory concentration ( IC50 ) values of
temozolomide and markedly decreased
temozolomide - induced apoptosis , all of which were abolished by selective
PI3 K inhibitor
BKM120 . -
pubmed
On the other hand , knockdown of
annexin A5 markedly decreased cell invasion ,
MMP-2 expression / activity ,
Akt phosphorylation at
serine 473 , and the IC50 values of
temozolomide and significantly increased
temozolomide - induced apoptosis . -
pubmed
In conclusion , our study provides the first evidence that
annexin A5 promotes
GBM cell invasion ,
MMP-2 expression / activity , and chemoresistance to
temozolomide through a
PI3K - dependent mechanism . -
pubmed
PURPOSE : To report a subgroup analysis comparing safety and effectiveness outcomes in Japanese and non - Japanese patients as part of a prospective , multinational , randomized controlled trial ( ClinicalTrials.gov identifier NCT00120406 ) evaluating a
paclitaxel - coated drug - eluting stent (
DES ) compared to percutaneous transluminal angioplasty (
PTA ) for treating
peripheral artery disease . -
pubmed
AIM : The treatment strategy for
steroid - resistant
nephrotic syndrome remains uncertain at present , especially in those with
calcineurin inhibitor resistance or intolerance . -
pubmed
A commercial
CSF vaccine ( C -
strain ) and
phosphate - buffered saline (
PBS ) were used as positive or negative controls , respectively . -
pubmed
On hydrophilic
Si , adhesion increased with increasing
RH for pollens with or without pollenkitt , up to 200nN at 70 % RH . -
pubmed
Additionally , 3.8 % of the
rifampin - resistant isolates showing no mutation in the
rpoB gene were significantly associated with mixed
infections ( chi(2 ) , 56.78 ; P & lt ; 0.01 ) . -
pubmed
This study revealed for the first time that Manu2 was the predominant group in the cases of mixed
infections , and this might be the main reason for heteroresistance and a possible mechanism for isolates without any mutation in the
rpoB gene to become
rifampin resistant . -
pubmed
The most interesting formulation , containing
GCS and
SBE - beta - CD , was denoted as DA
GCS / DA -
CD NPs . -
pubmed
DA
GCS /
DA - CD NPs showed
cytotoxic effect toward Olfactory Ensheathing Cells only at higher dosage . -
pubmed
Design and Synthesis of
Imidazopyrazolopyridines as Novel Selective
COX-2 Inhibitors . -
pubmed
In the present study , we introduced
imidazopyrazolopyridines as new potent and selective
COX-2 inhibitors that lack the standard pharmacophoric binding features to
hERG . -
pubmed
This work examined the potential of the anodic iontophoresis of
doxorubicin - loaded cationic solid lipid nanoparticles (
DOX -
SLN ) to increase the distribution and
tumor penetration of
DOX . -
pubmed
A double - labeled cationic
DOX -
SLN composed of the lipids
stearic acid and
monoolein and a new
BODIPY dye was prepared and characterized . -
pubmed
Although the iontophoresis of a
DOX solution increased the penetration of
DOX in the viable epidermis by approximately 4 - fold , the iontophoresis of cationic
DOX -
SLN increased the
DOX penetration by approximately 50 - fold . -
pubmed
In vivo , the
DOX -
SLN iontophoretic treatment was effective in inhibiting
tumor cell survival and
tumor growth and was accompanied by an increase in keratinization and consequent cell
death . -
pubmed
These results indicate a strong and synergic effect of iontophoresis with
DOX -
SLN and provide a potential strategy for the treatment of
skin cancer . -
pubmed
In recent years ,
gefitinib , an
epidermal growth factor receptor tyrosine kinase inhibitor , has shown its good antitumor activities in treating
NSCLC in a number of studies . -
pubmed
LncRNA
GClnc1 Promotes Gastric Carcinogenesis and May Act as a Modular Scaffold of
WDR5 and
KAT2A Complexes to Specify the
Histone Modification Pattern . -
pubmed
Mechanistically ,
GClnc1 bound
WDR5 ( a key component of
histone methyltransferase complex ) and
KAT2A histone acetyltransferase , acted as a modular scaffold of
WDR5 and
KAT2A complexes , coordinated their localization , specified the
histone modification pattern on the target genes , including
SOD2 , and consequently altered
gastric cancer cell biology . -
pubmed
High throughput chemical library screening identifies a novel
p110 - delta inhibitor that potentiates the anti -
myeloma effect of
bortezomib . -
pubmed
Genetic or pharmacologic inhibition of
p110 - delta substantially reduced
myeloma viability and enhanced cellular sensitivity to
bortezomib . -
pubmed
A
WD40 protein ,
AtGHS40 , negatively modulates
abscisic acid degrading and signaling genes during seedling growth under high
glucose conditions . -
pubmed
The
ghs40 - 1 mutant displayed severely impaired cotyledon greening and expansion and showed enhanced reduction in hypocotyl elongation of dark - grown seedlings when grown in
Glc concentrations higher than 3 % . -
pubmed
The
Glc -
hypersensitivity of
ghs40 - 1 was correlated with the hyposensitive phenotype of 35S::
AtGHS40 seedlings . -
pubmed
Quantitative real time - PCR analysis showed that
AtGHS40 was involved in the control of
Glc - responsive genes . -
pubmed
AtGHS40 acts downstream of
HXK1 and is activated by
ABI4 while
ABI4 expression is negatively modulated by
AtGHS40 in the
Glc signaling network . -
pubmed
Given that
AtGHS40 inhibited
ABA degrading and signaling gene expression levels under high
Glc conditions , a new circuit of fine - tuning modulation by which
ABA and
ABA signaling gene expression are modulated in balance , occurred in plants . -
pubmed
Thus ,
AtGHS40 may play a role in
ABA - mediated
Glc signaling during early seedling development . -
pubmed
The cells were also transfected with small interfering RNA directed against
PLTP , and
U0126 , an inhibitor of the
ERK1 / 2 pathway , was administered before CSE exposure . -
pubmed
Topoisomerase 1 (
TOP1 ) poisons like
camptothecin (
CPT ) are currently used in
cancer chemotherapy but these compounds can have damaging , off - target effects on neurons leading to
cognitive , sensory and motor deficits . -
pubmed
Neuronal cells expressed higher levels of
Top1 mRNA than fibroblasts but transcript levels are reduced in all cell types after treatment with
CPT . -
pubmed
Pathway analysis of the differentially expressed transcripts revealed activation of
ERK and
JNK signaling cascades in
CPT - treated MNs . -
pubmed
Immediate - early genes like
Fos ,
Egr-1 and
Gadd45b were upregulated in
CPT - treated MNs . -
pubmed
Fos mRNA levels were elevated in all cell types treated with
CPT ;
Egr-1 ,
Gadd45b and
Dyrk3 transcript levels , however , increased in
CPT - treated MNs and CNs but decreased in
CPT - treated fibroblasts . -
pubmed
Supplementation of drinking water with 40 mg / L ( 6 mg / kg BW )
boron also markedly increased splenic
IL-2 expression and the
CD4+/
CD8 + cell ratio and reduced splenic
CD8 + cell number . -
pubmed
Supplementation with 80 mg / L ( 12 mg / kg BW )
boron significantly increased
CD3 + and
PCNA+ cell numbers ( P & lt ; 0.05 ) and decreased the
IL-10 expression in the spleen . -
pubmed
Addition of 320 ( 48 ) and 640 ( 96 ) mg / L ( mg / kg BW )
boron markedly reduced the serum
IgG concentrations ; splenic
IL-2 and
IL-10 expression ; the number of CD3 + ,
CD4 + and PCNA+ cells ; and increased the number of splenic
CD8 + and
caspase-3 + cells and promoted
caspase-3 expression in CD3 + cells . -
pubmed
SUMMARY : Background FXaI16L (
PF-05230907 ) is a
zymogen - like variant of activated factor X (
FXa ) . -
pubmed
PREDICTORS :
Creatinine ,
cystatin C ,
beta - trace protein (
BTP ) , and
beta2 - microglobulin (
B2 M ) were measured in serum samples collected at the 12- and 24 - month follow - up visits , along with measured
GFR ( mGFR ) at these time points . -
pubmed
CONCLUSIONS : Declines in mGFR , eGFRcr , eGFRBTP , and the average of 4 filtration markers (
creatinine ,
cystatin C , BTP , and B2 M ) were consistently associated with progression to
ESRD . -
pubmed
In ~50 % of
prostate cancers , chromosomal rearrangements cause the fusion of the promoter and 5'-UTR of the
androgen - regulated
TMPRSS2 ( transmembrane
protease ,
serine 2 ) gene to the open reading frame of
ERG , encoding an ETS family transcription factor . -
pubmed
Here , we report a mechanism whereby
ERK - mediated phosphorylation of
ERG at one
serine residue causes a conformational change that allows
ERK phosphorylation at a second
serine residue ,
Ser-96 . -
pubmed
We found that the
Ser-96 phosphorylation resulted in dissociation of
EZH2 and
SUZ12 , components of
polycomb repressive complex 2 ( PRC2 ) , transcriptional activation of
ERG target genes , and increased cell migration . -
pubmed
Conversely , loss of
ERG phosphorylation at
Ser-96 resulted in recruitment of
EZH2 across the
ERG - cistrome and a genome - wide loss of
ERG - mediated transcriptional activation and cell migration . -
pubmed
Sialic acid - binding lectins (
SABLs ) are ubiquitous ancient molecules with binding properties to
N - acetyl or
N - glycolyl carbohydrates , and play crucial roles in both adaptive and innate immune responses . -
pubmed
Syndecan-1 (
Sdc1 ) is a major cell surface heparan
sulfate ( HS ) proteoglycan of epithelial cells , a cell type targeted by many bacterial pathogens early in their pathogenesis . -
pubmed
Synergistic antiproliferative effects of an
mTOR inhibitor (
rad001 ) plus
gemcitabine on
cholangiocarcinoma by decreasing
choline kinase activity . -
pubmed
This study aims to investigate a novel therapeutic regimen [
gemcitabine plus
everolimus (
rad001 ) , an
mTOR inhibitor ] for
cholangiocarcinoma . -
pubmed
In the in vitro study ,
gemcitabine plus
rad001 exerted a synergistic therapeutic effect on the
cholangiocarcinoma cells , irrespective of the
KRAS mutation status . -
pubmed
In the xenograft study ,
gemcitabine plus
rad001 showed the best therapeutic effect on
tumor volume change , and was associated with increased
caspase-3 expression , decreased
eIF4E expression , as well as overexpression of both
death receptor- and mitochondrial apoptotic pathway - related genes . -
pubmed
Also , increased intratumoral free
choline , decreased
glycerophosphocholine and nearly undetectable
phosphocholine levels were demonstrated by proton nuclear magnetic resonance , supported by results of decreased
choline kinase expression in western blotting . -
pubmed
We concluded that
gemcitabine plus
rad001 has a synergistic antiproliferative effect on
cholangiocarcinoma , irrespective of the
KRAS mutation status . -
pubmed
The antitumor effect is associated with activation of both
death receptor and mitochondrial pathways , as well as the downregulation of
choline kinase activity , resulting in a characteristic change in
choline metabolism . -
pubmed
Although
TCR specificity seems to be important for
CBZ - induced
SJS /
TEN in South East Asian patients , a distinct repertoire may not play a role in reactions to other drugs . -
pubmed
CPSase is a metabolic enzyme that is involved in
pyrimidine and
arginine synthesis . -
pubmed
Disruption of
carB resulted in survival rates that were reduced by up to 2,500 - fold following antibiotic treatment , and this phenotype was abolished by the addition of
uracil , highlighting the importance of de novo
pyrimidine biosynthesis for persister formation . -
pubmed
Disruption of
carB resulted in intracellular
ATP accumulation , and lowering
ATP levels using
arsenate restored the antibiotic tolerance profile of the mutant to levels similar to those seen with the wild type . -
pubmed
aeruginosa Disruption of
carB resulted in a metabolic perturbation that increased cellular
ATP and reduced persister formation . -
pubmed
While
PBA treatment reduced
ER stress and increased
collagen IV incorporation into BMs , the persistence of defects in BM structure and reduced ability of the BM to withstand mechanical stress indicate that
PBA may be counter - indicative for pathologies caused by matrix defects . -
pubmed
Furthermore , to evaluate the molecular mechanism involved in
cancer cell
death , we studied the genes that are involved in
fatty acid and
cholesterol biosynthesis pathway including
3 - hydroxy-3 - methylglutaryl - coenzyme A reductase (
HMGCR ) ,
Acetyl CoA synthase (
ACC ) ,
sterol regulatory element - binding protein (
SPREPB1 ) and
fatty acid synthase (
FASN ) respectively . -
pubmed
Conformational sampling and polarization of
Asp26 in pKa calculations of
thioredoxin . -
pubmed
Thioredoxin is a protein that has been used as model system by various computational methods to predict the pKa of
aspartate residue
Asp26 which is 3.5 units higher than a solvent exposed one ( eg ,
Asp20 ) . -
pubmed
Here , we use extensive atomistic molecular dynamics simulations of two different protonation states of
Asp26 in combination with conformational analysis based on RMSD clustering and principle component analysis to identify representative conformations of the
protein in solution . -
pubmed
For each conformation , the Gibbs free energy of proton transfer between
Asp26 and
Asp20 , which is fully solvated in a loop region of the
protein , is calculated with the Amber99sb force field in alchemical transformations . -
pubmed
The inclusion of the polarization of both
aspartate residues in the free energy cycle by
HI atomic charges corrects the results from the non - polarizable force field and reproduces the experimental DeltapKa value of
Asp26 . -
pubmed
Additionally , we investigated the effects of blocking
NMDA or non -
NMDA parietal ionotropic glutamate receptors on TUNL and found that , in contrast to the prefrontal cortex ,
NMDA receptors in the PPC are not necessary for TUNL performance , whereas blockade of
AMPA /
Kainate receptors significantly impaired accuracy . -
pubmed
Moreover , a significant increment ( p & lt ; 0.05 ) in the levels of
AST ,
ALP ,
creatinine , and
urea was evident in both experimental groups but more significant differences were noticed in the
tartrazine group . -
pubmed
Tapentadol is a novel analgesic molecule that synergistically combines two mechanisms of action ,
micro - opioid receptor agonism and
norepinephrine reuptake inhibition , and for which multiple literature data are available that confirm its efficacy and safety in controlling
pain . -
pubmed
The compounds namely
2 ( R ) , 4 ( R )- dihydroxypyrrolidine ,
4 - hydroxy-2 -( 4 - methoxyphenyl )- 1,1 - dioxo-3,4 - dihydrothieno [ 3,2 - e ] thiazine-6 - sulfonamide ,
2,3 - Diketo - L - gulonic acid ,
( 3 - hydroxy-2 - octadeca-9,12 - dienoyloxypropyl ) octadecanoate ,
2 -[[ 4 -[[ 4 -[( 4 - formamido-1 - methylimidazole-2 - carbonyl ) amino ]- 1 - methylimidazole-2 - carbonyl ] amino ]- 1 - methylimidazole-2 - carbonyl ] amino ] ethyl - dimethylazanium ,
Picolinic acid and
( 2 - Hydroxy-5 - nitrophenyl ) dihydrogen phosphate successfully bind inside the pocket of
survivin . -
pubmed
The presence of two reduced function variants in
CYP2C9 influences the acute response to
glipizide . -
pubmed
Glucose and
insulin levels were measured at baseline , 30 , 60 , 90 , 120 , 180 and 240 min for calculation of phenotypic endpoints of
glipizide response . -
pubmed
CONCLUSIONS : Carriers of two reduced function alleles in
CYP2C9 may experience an increased
insulin response to
glipizide and be predisposed to a higher risk of
hypoglycaemia , although no effect of genotype was seen in
glucose - based measurements . -
pubmed
Further studies are needed to clarify the utility of
CYP2C9 genotyping to guide
sulfonylurea treatment . -
pubmed
:
hypromellose phthalate (
HP ) ,
hypromellose (
AN ) , and hypromellose ( CN ) along with
acetic acid ( AA ) ( 0.1 M , 20 % w / v ) as a plasticizer . -
pubmed
The bioaccessibility of the total phenolic content and the total
flavonoids - including
decursin ( D ) and
decursinol angelate ( DA)-were significantly higher in
HP - AA - EAGN compared to the control . -
pubmed
In whole - cell voltage -
clamp recording , ASIC currents were evoked by brief local application of pH 6.0 external solution in the presence of
TRPV1 channel blocker
AMG9810 . -
pubmed
Construction of a recyclable
oxidase - mimicking
Fe3O4@
MnOx - based colorimetric sensor array for quantifying and identifying
chlorophenols . -
pubmed
Herein , we report a recyclable colorimetric sensor array according to the
oxidase - mimicking catalytic characteristics of
Fe3O4@
MnOx for the high - performance quantification and differentiation of typical CPs . -
pubmed
The core - shell
Fe3O4@
MnOx prepared by growing
oxidase - like
MnOx nanoflakes on the surface of magnetic
Fe3O4 particles via a hydrothermal process can exhibit excellent catalytic activity to trigger the color reaction of CPs and
4 - aminoantipyrine with the participation of
O2 . -
pubmed
Objectives : The aim of our study was to develop a simple , precise , sensitive and specific method for the simultaneous determination of
arbutin and
hydroquinone in different herbal slimming products using
GC -
MS . -
pubmed
Materials and Methods : The
methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of
arbutin and
hydroquinone using
GC -
MS method . -
pubmed
Here , a hybrid
PSC with a graded band gap is explored using
MAPbBr3 ( MA =
CH3NH3 ) and
MAPbBr0.9I2.1 quantum dots ( QDs ) as component cells . -
pubmed
Further analyses showed that
CvBV-7 - 1 and three homologs from other Cotesia bracoviruses possessed a C - terminal
leucine /
isoleucine - rich region and had a similar function in inhibiting melanization . -
pubmed
Our findings suggest that
CLP genes from Cotesia bracoviruses encoded proteins that contain a C - terminal
leucine /
isoleucine - rich region and function as melanization inhibitors during the early stage of parasitization , which is important for successful parasitization . -
pubmed
lncRNA -
PRLB Confers
Paclitaxel Resistance of
Ovarian Cancer Cells by Regulating
RSF1 /
NF - kappaB Signaling Pathway . -
pubmed
In this study , the authors explored
PRLB effect in the
paclitaxel (
Tax ) resistance of
ovarian cancer cells and revealed the role of
RSF1 (
remodeling and spacing factor 1)/
nuclear factor kappaB (
NF - kappaB ) signaling in this process . -
pubmed
Nowadays , LDs are viewed as dynamic organelles , which store and release
fatty acids depending on energy demand (
LD dynamics ) . -
pubmed
We aimed to quantify
PLIN2 and
PLIN5 content at individual LDs as a reflection of the ability to switch between
fatty acid release and storage depending on energy demand . -
pubmed
Herein , at first , its main active components were identified by UPLC - ESI - qTOF -
MS , and a representative MF
flavone glycosides profile consisting of ten compounds was illustrated , which is the most detailed one up to now . -
pubmed
Cholesterol glucosylation serves several functions that include promoting immune evasion , enhancing antibiotic resistance , maintaining the native helical morphology , and supporting functions of prominent virulence factors such as
CagA and
VacA. -
pubmed
Body weight , disease activity index (
DAI ) , colon length , biochemical parameters (
MDA and
SOD ) , and histopathological score in colon tissue were measured . -
pubmed
Metabolomics results revealed forty - nine differential metabolites as biomarkers of
DSS - induced
colitis , demonstrating that the path - mechanism of
colitis involved the perturbation of eight metabolic pathways , including
alpha - linolenic acid and
linoleic acid metabolism ,
sphingolipid metabolism ,
retinol metabolism ,
bile acid metabolism , et al . -
pubmed
Thirty - six biomarkers were especially reversed to normal - like levels by PQ - A via regulation of
alpha - linolenic acid and
linoleic acid metabolism , sphingolipid metabolism , and
retinol metabolism , which effectively hinted the potential pharmacological mechanism of
PQ - A related to
NF - kappaB /
p65 inflammatory signaling . -
pubmed
Molecular docking results predicted high affinity interaction between
PQ - A and
NF - kappaB /
p65 , involving
hydrogen - bond interactions at five
amino acid residues , suggesting
NF - kappaB / p65 as a target . -
pubmed
Butylated hydroxytoluene (
BHT ) and
butylated hydroxyanisole (
BHA ) at concentrations of 100 ppm ( 0.45 and 0.55 mM , respectively ) produced a marked leakage of
lactic dehydrogenase (
LDH ) from cultured myocardial and endotheloid cells into the culture medium . -
pubmed
Two and four days after adrenalectomy (
ADX ) on either day 5 or 10 after birth , a significant decrease in the concentration of
FSH was found ; 4 days after
ADX on either day 15 or 20 ,
FSH concentrations had increased significantly compared with sham - operated and/or intact controls . -
pubmed
It is argued that the effects of
ADX on both the release of
gonadotrophins and the onset of puberty are primarily , and presumably exclusively , due to the effects on general bodily development ( expressed in
body weight ) . -
pubmed
The enthalpy change for the oxidative deamination of
glutamate by
NADP+ catalyzed by
bovine liver glutamate dehydrogenase has been determined calorimetrically . -
pubmed
[ Molecular weight distribution of hydrolysis products of
polyuridylic acid by
cobra venom ribonuclease . -
pubmed
Onset of
spontaneous labor and changes in
E2 and
progesterone levels -- possible interrelation . -
pubmed
Angiotensin II or
endothelin-1 was continuously infused into the renal artery throughout the experiment and a bolus of
torasemide or
furosemide was injected into the bracheal vein . -
pubmed
infusion of
endothelin-1 , 1.5 ng kg-1 min-1 , produced effects similar to those of
angiotensin II on renal haemodynamics ; however , the onset of action was extremely slow compared with the effects produced by
angiotensin II . -
pubmed
alpha - Fetoprotein (
AFP ) production by 10(4 ) cells in 48 h increased in the A-10 Injection - treated and A-10 Injection+
CDDP - treated groups as the concentration of these agents increased . -
pubmed
Analysis of the sulfated molecules by
chondroitinase ABC digestion followed by composite
agarose /
acrylamide gel electrophoresis confirmed the proteoglycan nature of the molecules . -
pubmed
Immunoprecipitation of the
methionine - labeled extracts with a polyclonal antibody against the core protein indicated that the reduction in glycosaminoglycan synthesis was due to an inhibition of the core protein synthesis after
IL-1 treatment . -
pubmed
In this study we compared 2 putative biochemical markers for rejection of pancreas allografts , serum immunoreactive anodal
trypsinogen and urinary
amylase , with serum
creatinine in 15 simultaneously transplanted
type I diabetics . -
pubmed
These descending limbic structures , together with the amygdala , further send efferents to the four ascending NA , 5 -
HT , DA and
ACh systems . -
pubmed
Therefore , the coupling of these descending limbic structures to the ascending NA , 5 -
HT , DA and
ACh systems completes the neural circuits responsible for the consolidation and retrieval of new declarative memories . -
pubmed
ISDN and its two main hepatic metabolites ,
isosorbide-2 - mononitrate (
IS-2 - MN ) and
isosorbide-5 - mononitrate (
IS-5 - MN ) were compared for their ability to inhibit
platelet aggregation and
thromboxane B2 generation in vitro in response to threshold concentrations of
ADP ,
adrenaline ,
collagen ,
thrombin and
arachidonic acid . -
pubmed
Generation of a platelet - active species , IS-2 -
MN , and synergism with
prostacyclin are novel properties of
ISDN and may account for in vivo - in vitro differences in antiaggregatory properties and in vivo mechanism of action . -
pubmed
Effects of
estrogen on
progesterone receptor synthesis during endometrial cell culture . -
pubmed
The increase in the
progesterone receptor depended on the concentration of
estradiol , with maximal response at about 1 nM. -
pubmed
The
progesterone receptor response was specific for
estrogen . -
pubmed
Testosterone or
cortisol induced small or no response on the production of
progesterone receptors . -
pubmed
The
progesterone receptor synthesis was inhibited by
cycloheximide ( 10 micrograms / ml ) or
actinomycin D ( 10 micrograms / ml ) . -
pubmed
lysylbradykinin ) were inhibited in the presence of
thapsigargin , whereas
kinin responses were potentiated if the peptide was added following a stimulus which increases
cyclic AMP . -
pubmed
When examined by polarimetry , the material in all three fractions revealed native
collagen helical structure with melting points which ranged from 31 - 37 degrees C. When the fractions were denatured and rechromatographed on a column of
agarose beads , the most acidic fraction eluted as 13 - kDa polypeptides with and without prior reduction and alkylation . -
pubmed
Two 13 - kDa polypeptides , 13K2 and
13K3 , which did not contain any
methionyl residues and were not cleaved with
CNBr , were digested with
trypsin , and the peptide digests were resolved by reversed - phase chromatography . -
pubmed
To clarify the physiological role of
placental corticotropin - releasing hormone (
CRH ) , we measured plasma
CRH ,
ACTH , and
cortisol throughout pregnancy . -
pubmed
Maternal plasma
CRH levels , which increased progressively during pregnancy , correlated well with both
ACTH and
cortisol in early labor , delivery , and postpartum samples , and also with
cortisol levels in samples before labor . -
pubmed
Role of
insulin and counter - regulatory hormones in the control of hepatic
glycerolipid synthesis and low - density - lipoprotein catabolism in
diabetes . -
pubmed
We found that the
HA gene of the
H4 subtype is 1738 nucleotides in length and is predicted to encode a polypeptide of 564
amino acids . -
pubmed
The connecting peptide , which is removed from the precursor polypeptide by
peptidases to yield the mature
HA1 and
HA2 polypeptides , contains only one
basic amino acid . -
pubmed
Prelabeled
[ 3H ] AA platelets were stimulated with
thrombin or
collagen and the amount of free
[ 3H ] AA and
radiolabeled metabolites released were measured by high - performance liquid chromatography . -
pubmed
The reduction in free
[ 3H ] AA was accompanied by an increase ( P less than 0.01 ) in the percentage of
3H - radioactivity released as
cyclooxygenase products in atopic platelets ( compared to nonatopic cells ) after stimulation with 10 and 25 micrograms / ml
collagen . -
pubmed
Structural modifications of
metyrapone , an inhibitor of
cytochrome P-450 enzyme systems , have been made to determine the requirement for the
A - or B - pyridyl ring for inhibition of
P-45011 beta and
P-450 scc activities . -
pubmed
Characterization of the mitochondrial preparation demonstrated : enhancement of both activities by the addition of 15.0 microM adrenodoxin , the addition of 1 %
ethanol decreased both activities less than 10 % , and the apparent Km of
deoxycorticosterone for P-45011 beta was 6.8 microM and the apparent Km of
cholesterol for
P-450 scc was 21.6 microM. -
pubmed
Inhibition of
P-45011 beta and
P-450 scc activities with these compounds demonstrated : the
B - pyridyl ring of
metyrapone is required for inhibition of both activities whereas requirement for the A - ring is less stringent , and the four
metyrapone analogs were more selective inhibitors of
P-45011 beta activity . -
pubmed
Both types of granules stained for
basic protein and
arginine . -
pubmed
Comparison of
amino - acid sequences of hypothalamic peptide , brain - specific
histone and
myelin basic protein . -
pubmed
A comparative study of
interferon (
IFN ) production (
type - alpha and gamma ) was carried out using Ficoll -
hypaque purified fresh and cryopreserved mononuclear cells from eight normal healthy individuals . -
pubmed
Cells carrying one of the
phoA+ plasmids overproduce
alkaline phosphatase 10 - fold upon
phosphate limitation . -
pubmed
aeruginosa , which increased in the presence of the
iron - binding proteins
lactoferrin and
transferrin . -
pubmed
This bacteriostasis was reduced when the
iron - binding proteins were saturated with
iron . -
pubmed
We conclude that
opiate receptor stimulation by
morphine causes inhibition of the vagally mediated component of water - induced
bronchoconstriction . -
pubmed
The effect of
S-100a and
S-100b proteins and
Zn2 + on the assembly of brain microtubule proteins in vitro . -
pubmed
The homologous proteins
S-100a and
S-100b affect the microtubule system in a distinctly different way in the presence of low molar ratios of
Zn2 + . -
pubmed
Assembly of brain microtubule proteins can be almost completely inhibited and rapid disassembly can be induced by low molar amounts of
S-100b in the presence of low molar ratios [ 2 - 4 ] of
Zn2 + . -
pubmed
S-100a or
S-100b can not bind to the microtubule polymer - form , either in the absence or in the presence of
Zn2 + . -
pubmed
It is suggested that
HSG in
DES exposed women with
fertility disturbances be performed early in the process of
infertility investigation and cerclage should be considered when
uterine anomalies are demonstrated . -
pubmed
Cyclophosphamide (
CP ) is associated with significant
pulmonary toxicity ; however , the mechanism of toxicity is unknown . -
pubmed
Upon activation ,
CP produces
4 - hydroxycyclophosphamide ,
acrolein (
ACR ) and the alkylating metabolite ,
phosphoramide mustard . -
pubmed
These studies suggest 1 )
CP is not metabolized within pulmonary artery endothelial cells , 2 )
ACR may be the principal
CP metabolite involved in mediating direct
injury to pulmonary artery endothelial cells and 3 )
sulfhydryl - rich agents may be effective in reducing
CP - induced damage to critical endothelial cell barriers . -
pubmed
A study of
colchicine tubulin complex by donor quenching of fluorescence energy transfer . -
pubmed
The
tubulin -
colchicine complex was used as a donor - acceptor system to show that two inaccessible
tryptophans are at or near the
colchicine binding site . -
pubmed
PhoV consists of 550
amino acid residues , resulting in a molecular mass of 61.3 kDa . -
pubmed
In contrast to several other
alkaline phosphatases ,
PhoV was inhibited by
Mn2 + . -
pubmed
Angiotensin II ,
aldosterone and
endothelins play a central role in the remodeling of the ECM in
hypertension , and decrease
collagenase activity and/or increase
collagen synthesis in cultured cells . -
pubmed
Cytokines may stimulate or inhibit cell growth , the latter through
prostaglandin formation . -
pubmed
Cardiac fibroblasts possess G - protein - coupled
AT1 receptors for
angiotensin II that couple to activation of multiple signalling pathways , including :
phospholipase C - beta , with the subsequent release of
Ca2 + from intracellular stores and activation of
protein kinase C ,
mitogen - activated protein kinases ,
tyrosine kinases ,
phospholipase D ,
phosphatidic acid formation , and the
STAT family of
transcription factors . -
pubmed
Autoradiographic analysis of nondenaturing polyacrylamide gel electrophoregrams of outer membrane preparations previously incubated with [ 35Fe]
ferric vibrioferrin revealed a single radiolabeled band , in which the 78 - kDa protein was detected predominantly by
sodium dodecyl sulfate - polyacrylamide gel electrophoresis . -
pubmed
The treated cells lost most of their
iron uptake activity mediated by
vibrioferrin . -
pubmed
These results suggest that the
ferric vibrioferrin - binding protein of 78 kDa may function as the receptor for
ferric vibrioferrin involved in the initial step of
vibrioferrin - mediated
iron uptake . -
pubmed
The cells did not stain with acetylated
low density lipoprotein conjugated to the fluorescent marker
octadecyl indocarbocyanine , a marker for endothelial cells and monocytes . -
pubmed
10 - Formyltetrahydrofolate dehydrogenase (
10 - FTHFDH ) is a
folate - binding protein that is important in
folate metabolism . -
pubmed
Upon
isoproterenol stimulation , beta1- and
beta2AR down - regulated with a half - life of about 3 h and the
beta3AR with a half - life of 30 - 40 h. Long term stimulation with both saturating ( micromolar ) and nonsaturating ( nanomolar ) concentrations of beta - adrenergic agonists caused a complete desensitization of the beta - adrenergic response at the
adenylyl cyclase level and loss of stimulated
protein kinase A activity and
CREB phosphorylation . -
pubmed
These results suggest that
cAMP - dependent processes will be desensitized upon permanent treatment with
beta3AR agonists . -
pubmed
Prior to uptake into the cell
thiamin phosphates are first hydrolysed and
thiamin is taken up as free
vitamin which is then pyrophosphorylated by a
pyrophosphokinase . -
pubmed
The
THI genes encoding the enzymes of these final steps of
ThDP production and of
thiamin utilisation have been identified . -
pubmed
Their expression is controlled by the level of
thiamin and a number of regulatory proteins involved in regulated expression of the
THI genes are known . -
pubmed
Eleven such proteins have been found :
pyruvate decarboxylase (
Pdc , three isoforms ) ,
acetolactate synthase , a putative
alpha - ketoisocaproate decarboxylase with a regulatory role in
ThDP synthesis and two proteins of unknown function form the group of
Pdc related enzymes . -
pubmed
Surprisingly , it has been found recently that expression of one of the genes for
Pdc is repressed by
thiamin . -
pubmed
Reactivity of the dimple surface with specific
lectins suggests that
fucose might be utilized as a marker of
glycoproteins located at the dimple surface . -
pubmed
The
alpha - l - fucose - specific
lectin Ulex europaeus agglutinin I was utilized both in
lectin blotting and in affinity chromatography and cross - reacted with the 200- and 270/260 - kDa bands . -
pubmed
The proteolytic cleavage was significantly reduced by the addition of an excess amount of
l - arginine ( & gt;/=0.2 M ) to the culture medium , which resulted in a marked improvement in the yield of intact
hPTH . -
pubmed
The results demonstrated that
l - arginine at high concentrations reduced the rate of
hPTH proteolysis by inhibiting extracellular
proteases . -
pubmed
Using a combination of electrospray mass spectrometry , HPLC , direct sequencing , and family screening with
polymerase chain reaction / restriction digestion approach , we have characterized this
hemoglobin variant as resulting from a
Thr --> ;
Ala replacement at beta84(EF8 ) . -
pubmed
The data obtained are in agreement with differently aimed stress - induced alterations in the activity of the membrane
neutral sphingomyelinase : the key enzyme of the
sphingomyelin cascade , in the membrane
P2 fraction of the brain cortex . -
pubmed
The
IL-1 seems to participate in physiological mechanisms of realisation of stress reactions on the levels of its production and biological action on target cells as well as of the
sphingomyelin pathway of its signal transduction in nerve tissue . -
pubmed
Tissues have multiple proteolytic systems , including the
ATP- and
ubiquitin - dependent proteasome pathway . -
pubmed
Insulin inhibited
ATP- and
ubiquitin - dependent
lysozyme degradation more than 90 % by reticulocyte extract , in a dose - dependent manner ( IC50 approximately 50 nM ) . -
pubmed
Glycerol gradient analysis revealed cosedimentation of
IDE with the 20S proteasome and possibly with the 26S proteasome . -
pubmed
Serum levels of
alpha - tocopherol , lipid peroxidation products (
thiobarbituric acid ;
TBA ) ,
creatine kinase (
CK ) ,
lactate dehydrogenase (
LDH ) , and
LDH isozyme 1 - 5 were quantitatively analyzed . -
pubmed
These results indicate that
vitamin E supplementation can reduce the leakage of
CK and
LDH following 6 successive days of endurance running . -
pubmed
The ability of various types of
microcrystalline cellulose (
MCC ) to hold water when subjected to an applied force has been assessed by a centrifuge technique . -
pubmed
Results indicated that lung cells could activate DADDS to
dapsone and that , while the complex appeared to delay cell growth in the first 24 h period , no significant difference was seen between cells incubated in the presence and absence of the complex at 72 h. These results indicate that
DADDS - CD has significant potential as a drug - delivery system for DADDS in the lung based upon the ability of the cells to activate
DADDS . -
pubmed
The
protein composition of the stimulated cells was compared by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis and
tyrosine phosphorylation was detected by Western blot . -
pubmed
Calcium phosphate crystal - associated proteins :
alpha-2 - HS - glycoprotein ,
prothrombin fragment 1 and
osteopontin . -
pubmed
The incubation of
PPP with
thrombin in the presence of 100 - 1000 nM aptamer resulted in the significant concentration - dependent prolongation of
thrombin time ( up to fourfold , P<.0001 ) . -
pubmed
UNLABELLED : The development of
arterial hypertension is accompanied by impairment of the normal ratio of the "
ACE - depending " and of the sympathetic nervous system , correlates with the action on the main pharmacological " targets " :
I - I - imidazoline receptors (
moxonidine ) or the
ACE activity (
enalapril ) . -
pubmed
Spinal
PGE ( 2 ) application facilitated dorsal horn neuronal firing elicited by ionophoretic delivery of
NMDA , suggesting that an interaction of
PGs and
NMDA receptors may contribute to
inflammation - induced central sensitization . -
pubmed
RESULTS : Two different doses of
CHM could significantly increase the area of the peritoneal lymphatic stomata , promote its distributive density and enhance the drainage of urinary ion such as
sodium ,
potassium and
chlorine . -
pubmed
Meanwhile , the
NO concentration of two different doses of
CHM groups was 133.52+/-23.57 micromol / L and 137.2+/-26.79 micromol / L respectively . -
pubmed
The effect of Chinese herbal medicine on the peritoneal lymphatic stomata and the drainage of urinary ion was altered by adding
NO donor(
sodium nitropurruside ,
SNP ) or
NO synthase (
NOS ) inhibitor (
N ( G )- monomethyl - L - arginine ,
L - NMMA ) to the peritoneal cavity . -
pubmed
Maple syrup urine disease (
MSUD ) is an
inborn error of metabolism stemming from a deficiency in
2 - ketoacid dehydrogenase and resulting in the systemic accumulation of branched chain
amino acids (
BCAAs ) . -
pubmed
Remarkably , however , digit duplication by
dHAND requires neither the transcriptional activation domain nor the
basic region , but only the HLH motif . -
pubmed
More than one
caspase was involved and these
caspases appeared to transmit parallel signals for apoptosis induction because only broad - spectrum
caspase inhibitors , but not inhibitors for specific individual
caspases inhibited apoptosis in
choline- or
methionine - deprived cells . -
pubmed
reinhardtii CC-2341 ( ac - u - g-2.3 ) , a
PSI - minus
strain , fluorescence increased with
chlorophyll without treatment with
DCMU . -
pubmed
We therefore expressed and tested
CV - N homologs in which the glycosylation - susceptible
Asn residue at position 30 was replaced with
Ala ,
Gln , or
Val , and/or the
Pro at position 51 was replaced by
Gly to eliminate potential conformational heterogeneity . -
pubmed
Immunofluorescence studies on transiently transfected cells indicated membrane localization and indicated that both
NH ( 2 )- and COOH - terminal tails of the
SLC26A6 variants are located intracellularly , suggesting a topology with an even number of transmembrane domains . -
pubmed
We demonstrated also that the
COOH terminus of
SLC26A6 binds to the first and second PDZ domains of the
Na (+)/ H (+ ) exchanger ( NHE ) 3 kinase A regulatory protein (
E3KARP ) and
NHE3 regulatory factor (
NHERF ) proteins in vitro . -
pubmed
Truncation of the last three
amino acids ( TRL ) of
SLC26A6 abrogated the interaction but did not affect transport function . -
pubmed
CONCLUSIONS : This study suggests that outpatient induction of labour with intravaginal
CR -
PGE2 may be a reasonable option for selected low - risk women ; however , further study is needed to confirm the safety of this approach . -
pubmed
We set the conditions where CEF -
PSII functioned or did not , by adding
nigericin to the reaction mixture for the dissipation of
DeltapH across thylakoid membranes , and then the thylakoids were illuminated . -
pubmed
These results imply that
CEF -
PSII contributes to the protection of
PSII from its photoinhibition with
LEF , as an electron sink . -
pubmed
,
imatinib mesylate or
interferon - alpha ) entails the risk of the development of a sudden blastic phase , which may not permit enough time to perform a
SCT . -
pubmed
IL-6 levels appeared to correlate positively with
bilirubin and
gamma - GT levels and negatively with the degree of
acidosis . -
pubmed
UNSPECIFIC STAINING OF
PARAPROTEINS BY
PICRIC ACID . -
pubmed
In this study , we evaluated the role of
Ras in
Ag - induced
airway inflammation and
hyperresponsiveness by i.p . -
pubmed
OVA challenge also caused
airway hyperresponsiveness to
methacholine , which was dose dependently blocked by treatment with
TAT - dnRas . -
pubmed
TAT - dnRas also blocked
Ag - induced
IL-4 and
IL-5 , but not
IFN - gamma , production in lung tissue . -
pubmed
The latter include
lecithinase positive Clostridium ,
citrate assimilating enterobacteria , as well as Escherichia with low biochemical activity and capacity for
hemolysin production . -
pubmed
The
cytokine response profiles as determined by
[ ( 3 ) H ]- thymidine incorporation assay were : 2 - to-12 fold growth stimulation of
MUTZ-11 by
GM - CSF ,
IFN - alpha (
interferon ) ,
IFN - beta ,
IFN - gamma ,
IL-3 and
SCF (
stem cell factor ) ; growth inhibition by
TGF - beta1 (
transforming growth factor ) ,
TNF - alpha (
tumor necrosis factor ) and
TNF - beta . -
pubmed
The transcription intensity of
LeFRO1 in roots is dependent on the
iron status whereas it is constitutively expressed in leaves . -
pubmed
These results indicate that
LeFRO1 is required in roots and shoots as well as in reproductive organs for
iron homeostasis and that its transcription in roots and shoots is regulated by different control mechanisms . -
pubmed
Acute administration of
gamma - aminobutyric acid B ( GABAB ) receptor agonists decreased
nicotine ,
cocaine ,
ethanol , and
heroin self - administration . -
pubmed
The present study investigated the effects of repeated subcutaneous administration of the
GABAB receptor agonist
CGP44532 on
nicotine- and food - maintained responding under a fixed ratio 5 schedule of reinforcement . -
pubmed
Two
PPARgamma ligands ,
15 - deoxy - delta12,14 - prostaglandin J2 (
PGJ2 ) and
rosiglitazone , inhibited growth of all cell lines , with
PGJ , being the most potent agent . -
pubmed
PGJ2 , but not
rosiglitazone , induced arrest of the cells in the
G2 / M phase as well as apoptosis . -
pubmed
CYP2C8 and
CYP3A4 are the major
enzymes responsible for the metabolism of
cerivastatin , and a transporter ,
OATP2 , contributes to uptake of
cerivastatin to the liver . -
pubmed
To our knowledge , this is the first report showing that the adverse effect of
cerivastatin might be caused by the genetic variant of
CYP2C8 . -
pubmed
Using RT - PCR , we determined the expression of angiogenic regulators in
DMBA - induced
intraductal hyperplasia (
IDP ) ,
carcinoma in situ ( CIS ) , invasive
tumors (
INV ) , as well as normal tissue . -
pubmed
Inhibition of
IGF-1R by the
tyrphostin ,
AG1024 , blocked endothelial tubulogenesis in vitro , confirming that
IGF-1 functions as a regulator of angiogenesis . -
pubmed
Lipid surface density and the surface structure of multilamellar ( MLVs ) and extruded unilamellar ( LUVs ) vesicles deposited on three types of
polystyrene ( PS ) well - plates were probed by fluorescence and atomic force microscopy (
AFM ) imaging . -
pubmed
Pharmacological inhibition of
calcineurin , by the immunosuppressive drugs
cyclosporin A and
FK506 , affects a wide variety of diseases . -
pubmed
The thermal
TMS peroxide conversions documented provided biomimetic models for enzymatic conversions of
fatty acid hydroperoxides and also offered a method to generate an array of
oxylipin derivatives of value as reference compounds in
GC -
MS studies . -
pubmed
Comparing the wild - type
strain , which is positive in
beta - glucuronidase activity , with the mutated
strains named clone1 and clone2 in laboratory conditions , we observed that
beta - glucuronidase influences the production of
hyaluronic acid considerably and the molecular weight of hyaluronan slightly . -
pubmed
The significant increase in the production of
hyaluronic acid and the slight increase in the molecular weight are probably caused by an absence of free
beta - glucuronic acid , due to its removal from the non - reducing termini of the polysaccharide by
beta - glucuronidase . -
pubmed
Association of
amino acid variants in the
activating transcription factor 6 gene (
ATF6 ) on 1q21 - q23 with
type 2 diabetes in Pima Indians . -
pubmed
Although the associations with
type 2 diabetes and plasma
insulin levels are marginal and their functional consequences are yet unknown , all three
amino acid substitutions are located in a functionally important part of
ATF6 . -
pubmed
12 - Lipoxygenase utilizes
arachidonic acid to synthesize
12 ( S )- hydroperoxyeicosatetraenoic acid , which is converted to the end product
12 ( S )- hydroxyeicosatetraenoic acid , an
eicosanoid that promotes
tumorigenesis and
metastasis . -
pubmed
The higher concentrations of
SNP up - regulated the apoptosis - related protein expression , which is consistent with the analyses of sub - G(1 ) phase arrest ,
annexin V -
FITC (
fluorescein isothiocynate ) staining , nuclear staining , and DNA fragmentation . -
pubmed
Improvement of
pulmonary function with
imatinib mesylate in
bronchiolitis obliterans following allogeneic hematopoietic cell transplantation . -
pubmed
Recent studies suggest that
beta - escin may possess anti - inflammatory , anti -
hyaluronidase , and anti -
histamine properties . -
pubmed
Results also indicate that
beta - escin inhibited growth of
colon cancer cells with either wild - type or mutant
p53 . -
pubmed
This chemistry mimics that known for the
copper enzyme dopamine - beta - monooxygenase . -
pubmed
Design of antisense
oligonucleotides and short interfering RNA duplexes ( siRNA ) targeted to
BCL6 mRNA : towards rational drug development for specific
lymphoma subsets . -
pubmed
GTPase activating proteins (
GAPs ) help the
GTPase return to the inactive
GDP - bound form . -
pubmed
GxcDD can bind to several
Rac GTPases , but its function as a
nucleotide exchange factor needs to be studied further . -
pubmed
Aromatic compounds were UV irradiated ( 254 nm , 15 W ) to generate
hydrogen peroxide , which was determined via PO - CL detection using a mixture of
bis ( 2,4,6 - trichlorophenyl ) oxalate (
aryloxalate ) and
2,4,6,8 - tetrathiomorpholinopyrimido [ 5,4 - d ] pyrimidine ( fluorophore ) as a
post - column CL reagent . -
pubmed
Generation of
hydrogen peroxide from aromatic compounds was confirmed using a flow injection analysis ( FIA ) system incorporating an
enzyme column reactor immobilized with
catalase . -
pubmed
Dipeptide derivatives bearing various P2 residues and
pyrrolidine derivatives as P1 mimics were evaluated in order to identify lead structures for the development of
DPP8 and
DPP9 inhibitors . -
pubmed
After release of the SMA
clamp ,
AM ( 12 mug / kg BW ) and
AMBP-1 ( 40 mug / kg BW ) in combination or vehicle ( 1 - ml normal saline ) were administered intravenously over a period of 30 min . -
pubmed
The mucosal barrier function in the small intestine was assessed in an isolated everted ileum sac using
fluorescein - isothiocyanate dextran ( FD4 ) at 4 h after
AM /
AMBP-1 treatment . -
pubmed
AIMS : There is no cure for
pulmonary arterial hypertension , but current approved treatment options include
prostanoids ,
endothelin - receptor antagonists , and
phosphodiesterase type-5 inhibitors . -
pubmed
Multiplex ligation - dependent probe amplification screening of isolated increased
HbF levels revealed three cases of novel rearrangements / deletions in the beta -
globin gene cluster . -
pubmed
We selected 15 individuals with elevated
fetal haemoglobin (
HbF ) levels ( & gt;5 % ) from a previous screening of 27 006 Korean individuals and analysed dosage changes of the
globin gene cluster using multiplex ligation - dependent probe amplification ( MLPA ) . -
pubmed
Three subjects were found to have deletions in the
globin gene cluster , including a
beta - thalassaemia due to deletion of
HBB ( beta -
globin gene ) , an HPFH due to deletions of
HBD ( delta -
globin gene ) and
HBB , and an HPFH due to a novel
HBG2 -
HBG1 fusion gene consisting of exons 1 and 2 of
HBG2 ( ( G)gamma - globin gene ) and exon 3 of
HBG1 ( ( A)gamma -
globin gene ) . -
pubmed
The
protein was crystallized using
di [ poly ( ethylene glycol ) ] adipate , which also served as a cryoprotectant . -
pubmed
The CB sections were prepared using the
sodium alginate method and subjected to
HE , PAS - AB and immunohistochemical stains . -
pubmed
Here we report the discovery of newly designed and synthesized
pyridylpyrimidine based potent and selective inhibitors of
HIF-1alpha . -
pubmed
Although
dyslipidemia is the most influential and least treated factor , the greatest benefit for
CVD prevention is obtained with integrated intervention on all risk factors , reducing blood pressure to below 140/80 mmHg ,
low - density lipoprotein cholesterol (
LDL - c ) to below 100 mg / dl , encouraging smoking cessation , regular physical activity and maintaining a healthy weight (
BMI & lt ; 25 kg / m2 ) . -
pubmed
Here , we used single molecule force spectroscopy techniques to directly determine the mechanical strength of such highly covalent
ferric -
thiolate bonds in
rubredoxin . -
pubmed
Release was evoked by either
veratridine ( 1 , 3.2 , or 10 muM ) , which prevents activated voltage - dependent
Na (+ ) channels from closing , or elevation of extracellular [
K (+ ) ] from 3 to 15 mM. -
pubmed
The exocytosis inhibitor
tetanus toxin (
TeT ) or withdrawal of buffer
Ca ( 2 + ) (
Ca ( e ) ( 2 + ) ) reduced K(+)-evoked release in both species , while blockade of
Na (+ ) channels with
tetrodotoxin had no effect . -
pubmed
In addition , oxidative damage in the vessel wall was determined by measuring
malondialdehyde concentrations , vascular
superoxide production and
SOD activity . -
pubmed
The exaggerated vascular contractions were inhibited by both
apocynin ( a
Nox inhibitor ) and
tempol ( a
SOD mimetic ) in a concentration - dependent manner . -
pubmed
The
nicotine treatment significantly decreased the
superoxide dismutase activity and increased
malondialdehyde ,
superoxide and
nitrotyrosine protein levels in the vascular wall . -
pubmed
Consistently , antenatal
nicotine exposure significantly enhanced the protein expression of
NADPH oxidase Nox2 /
gp91 , but not
Nox4 in the aorta . -
pubmed
CONCLUSIONS AND IMPLICATIONS : The present findings suggest that antenatal
nicotine exposure results in the programming of heightened oxidative stress and vascular
hypertensive reactivity via a
Nox2 - dependent mechanism , leading to an increased risk of
hypertension in adult offspring . -
pubmed
This relative decrease in activity could be mainly related to increases in islet asymmetric
dimethyl - arginine content , an endogenous inhibitor of
nNOS activity . -
pubmed
Furthermore ,
17AAG blocked the interaction between
Hsp90 and
TGF - beta type II receptor (
TbetaRII ) and promoted ubiquitination of
TbetaRII , leading to the decreased availability of
TbetaRII . -
pubmed
Smurf2 - specific siRNA reversed the ability of
17AAG to inhibit
TGF - beta1 signaling . -
pubmed
The effect of
17AAG on
TbetaRII expression and
renal fibrosis was confirmed in UUO kidneys . -
pubmed
METHODS : Sinus grafts were performed by using granules of a HA /
TTCP blend and a
collagen membrane . -
pubmed
Apocynin ,
manganese ( III ) tetrakis ( 1 - methyl-4 - pyridyl ) porphyrin ,
catalase , and
diethyldithiocarbamate inhibited endothelium - dependent contractions . -
pubmed
Biosimilars of
filgrastim in autologous stem cell transplant : reduction in
granulocyte - colony stimulating factor costs , but similar effects on bone marrow recovery . -
pubmed
These results indicate that the
CA1 dopamine D1 receptors may potentially play an important role in
scopolamine - induced
amnesia as well as the
scopolamine state - dependent memory . -
pubmed
Furthermore , our results propose that
dopamine D1 receptor agonist ,
SKF38393 reverses the
scopolamine - induced
amnesia via
acetylcholine release and possibly through the activation of
muscarinic receptors . -
pubmed
Alpha-1 - adrenergic receptors in
heart failure : the adaptive arm of the cardiac response to chronic
catecholamine stimulation . -
pubmed
Alpha-1 - adrenergic receptors (
ARs ) are
G protein - coupled receptors activated by
catecholamines . -
pubmed
There are far fewer
alpha-1 - ARs than
beta - ARs in the nonfailing heart , but their abundance is maintained or increased in the setting of
heart failure , which is characterized by pronounced chronic elevation of
catecholamines and
beta - AR dysfunction . -
pubmed
Seawater
chromium ,
copper ,
manganese ,
nickel and
zinc concentrations were measured , as well as metal accumulation , lipid peroxidation , protein oxidation , reduced
glutathione levels , and enzymatic activities of
superoxide dismutase and
glutathione - S - transferase in gills and digestive glands . -
pubmed
The main components of this
disulfide relay machinery are the
oxidoreductase Mia40 and the
sulfhydryl oxidase Erv1 /
ALR . -
pubmed
INTERVENTION(S ):
Goserelin administered on day 13 of life ,
CTX on day 18 of life , euthanasia on day 20 ( prepubertal ) , 56 ( pubertal ) , or 92 of life ( mature ) , measurements of
body weight , length , uterine weight , serum antimullerian hormone and
follicle - stimulating hormone , and histologic assessment of ovarian follicles and femur growth , and apoptotic rates by TUNEL . -
pubmed
The nerve regeneration was systematically characterized by sciatic function index ( SFI ) , histological assessment including
HE staining , immunohistochemistry ,
ammonia silver staining , Masson 's trichrome staining and
TEM observation . -
pubmed
Novel
CT techniques , including
adenosine stress
CTP and TAG320 , may improve the diagnostic accuracy of
CTA . -
pubmed
Following on the success of
Aripiprazole with its high clinical efficacy and minimal side effects , further antipsychotic drugs ( such as
Bifeprunox ) have been developed based on the same
dopamine D2 partial agonist pharmacological profile as
Aripiprazole . -
pubmed
The analysis was performed on a column of ultimate
C18 ( 50 mm x 4.6 mm , 5 microm ) with the mobile phase consisting of
methyl alcohol -
water -
formic acid = 75:25 : 0.05 at a flow rate of 0 . -
pubmed
The areal density of
Cu in
CA1 decreased with the cumulative ( totaled over 21 stimulation days ) intensity and duration of electroshock - induced tonic
seizures while
Zn level in the hilus of DG was positively correlated with the duration and intensity of both
tonic and clonic seizures . -
pubmed
Exosomes enhanced ubiquitination of cellular proteins , including
CD36 , and blockade of proteosome proteolysis with
MG-132 rescued
CD36 expression . -
pubmed
The summary statistics indicated that
calcium dobesilate was significantly associated with improving
retinal microaneurysms ( RR : 0.62 , 95%CI : 0.42 - 0.90 , P=0.01 ) ,
retinal hemorrhages ( RR : 0.39 , 95 % CI : 0.17 - 0.88 , P=0.02 ) ; exudates ( RR : 0.31 , 95 % CI : 0.12 - 0.81 , P=0.02 ) , reduction of whole blood viscosity ( MD : -0.57
CP , 95 % CI : -0.75 to -0.38 , P<0.001 ) , plasma viscosity ( MD : -0.36
CP , 95 % CI : -0.63 to -0.09 , P=0.01 ) and blood
cholesterol ( MD : -0.48 mg mL(-1 ) , 95 % CI : -0.64 - 0.33 , P<0.00001 ) . -
pubmed
The other metabolites of cosmeceutical , medicinal , nutraceutical and industrial significance such as
alpha - tocopherol ,
n - methylpyrrolidone (
NMP ) ,
trans - farnesol ,
prostaglandin F2 ,
protocatechuic , gallic and cinnamic acids were identified from non - aqueous extracts using
GC -
MS . -
pubmed
omega-3FFA and
tamoxifen significantly increased
Erk1 / 2 and
Akt phosphorylation levels in a dose and time dependent manner . -
pubmed
Compared to
omega-3FFA alone , the combination of
tamoxifen with
omega-3FFA significantly increased
Erk1 / 2 and
Akt phosphorylation levels . -
pubmed
Protein instability in supercritical
CO2 limits the application of this green solvent in
enzyme - catalyzed reactions . -
pubmed
Molecular dynamics simulation is used to monitor the effects of adding different natural osmolytes on the conformation and dynamics of
chymotrypsin inhibitor 2 ( CI2 ) in supercritical
CO2 . -
pubmed
The
NAD(+)-dependent deacetylase
Sirt1 has been reported to play positive roles in the regulation of cellular homeostasis in response to various stresses . -
pubmed
Although the
copper - catalyzed
azide -
alkyne cycloaddition reaction efficiently introduced 237 copies of
GM2 per Qbeta , this construct failed to induce significant amounts of
anti - GM2 antibodies compared to the Qbeta control . -
pubmed
In contrast ,
GM2 immobilized on Qbeta through a
thiourea linker elicited high titers of
IgG antibodies that recognized
GM2 - positive
tumor cells and effectively induced cell lysis through complement - mediated
cytotoxicity . -
pubmed
Cystathionine beta synthase (
CBS ) is a key enzyme in the
methionine and
cysteine metabolic pathway , acting as a metabolic gatekeeper to regulate the flow of fixed
sulfur from
methionine to
cysteine . -
pubmed
Mutations in the
CBS gene cause clinical
CBS deficiency , a disease characterized by elevated plasma total
homocysteine ( tHcy ) and
methionine and decreased plasma
cysteine . -
pubmed
The results suggest that the altered pharmacokinetics of
DIC might be attributed to
RSV mediated inhibition of
CYP2C9 enzyme . -
pubmed
AIM : The study aimed to determine whether Coca - Cola ( Coke ) Zero is a safe and effective solvent for
polyethylene glycol (
PEG ) . -
pubmed
Tryptophan Fluorescence Changes Related to
Ca ( 2 +)- ATPase Function . -
pubmed
We demonstrate here with
Ca ( 2 + ) and two specific inhibitors of
SERCA how this can be done by measurements of the intrinsic fluorescence of the
tryptophan residues of
SERCA . -
pubmed
Optimum coagulant for coagulation for
DON and
TDN removals was characterized by the dense convex - concave packing structure differing from other
zinc - based coagulant , polycrystalline structure and high content colloidal species , which could account up to 87 % of the total colloidal species . -
pubmed
DON removal efficiency was also higher than and linearly correlated with total dissolved
nitrogen (
TDN ) removal , which implies that an effective coagulation for
TDN is also effective for
DON . -
pubmed
The electron donation to
PSI was substantially reduced in
iron - deficient cells . -
pubmed
This decreased electron transfer may induce the over - reduction of the
plastoquinone pool and consequently the appearance of acceptor side photoinhibition in
PSII even at low light intensities . -
pubmed
[ Effects of laser solid forming of porous
titanium on proliferation of osteoblast and
RANKL /
OPG expression ] . -
pubmed
OBJECTIVE : To evaluate the effect of laser solid forming (
LSF ) of porous
titanium on
receptor activator of NF - kappaB ligand (
RANKL)/
osteoprorotegerin (
OPG ) expression and osteoblast cells growth . -
pubmed
An
Oxidoreductase AioE is Responsible for Bacterial
Arsenite Oxidation and Resistance . -
pubmed
In this study , we found that
aioE is induced by
AsIII and located in the
arsenic island near the
AsIII oxidase genes
aioBA and
co - transcripted with the
arsenic resistant genes
arsR1 -
arsC1 -
arsC2 -
acr3 - 1 . -
pubmed
AioE belongs to
TrkA family corresponding the electron transport function with the generation of
NADH and
H + . -
pubmed
An
aioE in - frame deletion
strain showed a null
AsIII oxidation and a reduced
AsIII resistance , while a
cytC mutant only reduced
AsIII oxidation efficiency . -
pubmed
With
AsIII ,
aioE was directly related to the increase of
NADH , while
cytC was essential for
ATP generation . -
pubmed
In addition , cyclic voltammetry analysis showed that the redox potential ( ORP ) of
AioBA and
AioE were +0.297 mV vs.
NHE and +0.255 mV vs.
NHE , respectively . -
pubmed
The ORP gradient is
AioBA & gt ;
AioE & gt ; CytC ( +0.217 ~ +0.251 mV vs.
NHE ) , which infers that electron may transfer from
AioBA to
CytC via
AioE. -
pubmed
The results indicate that
AioE may act as a novel
AsIII oxidation electron transporter associated with
NADH generation . -
pubmed
Since
AsIII oxidation contributes
AsIII detoxification , the essential of
AioE for
AsIII resistance is also reasonable . -
pubmed
In this study , we found that it is possible to confer antimicrobial activity to
Au NPs through precise control of their size down to
NC dimension ( typically less than 2 nm ) . -
pubmed
The
peroxidase mimics , the
CeO2 nanoparticles , as signal generators were integrated with the detection antibody . -
pubmed
BACKGROUND : Interest in
technetium-99 m ( 99mTc )- sestamibi imaging for neoadjuvant chemotherapy (
NAC ) response monitoring in locally advanced
breast cancer ( LABC ) is increasing but remains matter of discussion . -
pubmed
Resistance to the integrase strand transfer inhibitors
raltegravir and
elvitegravir is often due to well - identified mutations in the
integrase gene . -
pubmed
Furthermore , most in vitro experiments to select resistance to
dolutegravir have resulted in few mutations of the
integrase gene . -
pubmed
Mutations selected in vitro by
dolutegravir , located outside the
integrase gene , can confer a high level of resistance to all
integrase inhibitors . -
pubmed
Most mutations selected by
raltegravir ,
elvitegravir , or
dolutegravir exposure are located inside the catalytic site of the
integrase gene , but mutations outside the catalytic site of the
integrase gene have also been selected with
dolutegravir . -
pubmed
Following in vitro selection with
dolutegravir , we report , for the first time , a virus with selected mutations outside the HIV-1
integrase gene that confer resistance to all
integrase inhibitors currently used to treat patients , such as
raltegravir ,
elvitegravir , and
dolutegravir . -
pubmed
Our observation may explain why some viruses responsible for
virological failure in patients treated with
dolutegravir did not show mutations in the
integrase gene . -
pubmed
This trial compared
dalcetrapib , the
cholesteryl ester transfer protein inhibitor , with placebo in patients with recent
ACS and was performed between April 2008 and September 2012 at 935 sites in 27 countries . -
pubmed
There was broad application of evidence - based secondary prevention strategies after
ACS , including use of
statins in 4030 patients ( 97 % ) . -
pubmed
Conclusions and Relevance : For patients with recent
ACS who are treated with
statins ,
Lp ( a ) concentration was not associated with adverse cardiovascular outcomes . -
pubmed
BACKGROUND : A novel auto - cross - linked
hyaluronic acid formulation ( HA -
ACP ) has been proposed as a treatment for skin rejuvenation . -
pubmed
Characterization of Melanin Radicals in
Paraffin - embedded Malignant
Melanoma and
Nevus Pigmentosus Using X - band
EPR and
EPR Imaging . -
pubmed
The
EPR results revealed that the peak - to - peak linewidths ( DeltaHpp ) of
paraffin - embedded MM and
NP samples were 0.65 + - 0.01 and 0.69 + - 0.01 mT , respectively . -
pubmed
Here , we performed replica exchange molecular dynamics simulations of
Abeta1 - 42 in explicit water to study , at the molecular level , the effect of CLR01 binding to the
lysine and
arginine residues in the dimer . -
pubmed
We found that CLR01 molecules encapsulate both
lysine residues of each
Abeta1 - 42 monomer while only establishing labile interactions with the
arginine residues . -
pubmed
To develop a safe SPE for energy storage applications , mechanically robust cross - linked
epoxy matrix is combined with fast ion - diffusing ionic liquid /
lithium salt
electrolyte ( ILE ) via a simple one -
pot curing process . -
pubmed
The following type 2
inflammation markers were assessed : exhaled
nitric oxide ( FeNO ) , serum
periostin ,
serum eosinophil cationic protein (
S - ECP ) , and urinary eosinophil - derived neurotoxin ( U -
EDN ) . -
pubmed
RESULTS : Elevated U -
EDN ( values in the highest tertile , & gt;=65.95 mg / mol
creatinine ) was more common in subjects with fixed - AO vs. subjects without fixed - AO : 55 % vs. 29 % , P & lt ; 0.001 . -
pubmed
Elevated U -
EDN related to increased likelihood of having fixed - AO in both all subjects and never - smoking subjects , with adjusted ( adjusted for sex , age group , use of inhaled
corticosteroids last week ,
atopy , early - onset
asthma , smoking history , and packyears ) odds ratios ( aOR ) of 2.38 ( 1.28 - 4.41 ) and 2.51 ( 1.04 - 6.07 ) , respectively . -
pubmed
We previously synthesized a novel biogenic
nanoselenium ( BNS ) particles and proved that BNS could effectively improve intestinal antioxidative function through activating
Nrf2 - ARE pathway . -
pubmed
BNS particles significantly increased the content of phosphorylated -
Nrf2 , without evident influence on the level of
Kelch - like ECH - associated protein 1 (
Keap1 ) . -
pubmed
Moreover ,
BNS also induced the activation of
p38 ,
extracellular signal - regulated kinase 1 / 2 (
ERK1 / 2 ) ,
c - Jun N - terminal kinase , and
AKT while phosphorylating
Nrf2 . -
pubmed
Overall , our data demonstrated that
BNS particles activated
Nrf2 - ARE pathway through
p38 ,
ERK1 / 2 , and
AKT mediated - phosphorylation of
Nrf2 to improve the antioxidant function of intestinal epithelial cells . -
pubmed
This study aimed to develop a novel dressing material for
DS containing the phenolic agent
guaiacol and evaluate its biological properties . -
pubmed
Protein kinase C (
PKC ) is a class of phospholipid - dependent
serine /
threonine kinases that contribute to cell survival , migration , and invasion . -
pubmed
However , the role of
PKC in
glucose - stimulated
insulin secretion ( GSIS ) remains unclear . -
pubmed
Finally , we showed that
TRPC1 could reverse the decrease in intracellular
Ca2 + levels and reduced
insulin secretion induced by treatment with
PKCalpha inhibitor under high
glucose conditions . -
pubmed
Nucleotide - binding oligomerization domain , leucine rich repeat , and
pyrin domain - containing protein 3 (
NLRP3 ) inflammasome has been implicated in a series of physiological and
pathological processes . -
pubmed
Interference with
rosuvastatin in vitro revealed that the expression of
NLRP3 inflammasome and its downstream
cytokines were significantly downregulated in both
SAP and
AMI groups in a time - dependent manner . -
pubmed
The activation of
NLRP3 inflammasome may be involved in the development of
CHD , and
rosuvastatin could attenuate the inflammatory process of
atherosclerosis by downregulating
NLRP3 expression and its downstream mediators . -
pubmed
These findings indicated a potential role of
NLRP3 in the pathogenesis and management of
CHD , and also provided new insights into the mechanistic framework of
rosuvastatin activity . -
pubmed
A DNA radar was first built by assigning the
silver nanocluster ( AgNC ) as the radar transmitter , the middle single strand DNA - bridge connected on the AgNCs as the electromagnetic wave , and the
G4 / crystal violet complex as the radar antenna . -
pubmed
Here ,
G4 is chosen as the suitable template to connect potential nanomaterial AgNCs with the
G4 binder ( crystal violet , CV ) since the rich
guanine in
G4 could not only enhance the fluorescence of AgNCs but also form quartets offering powerful binding sites for the
G4 binder . -
pubmed
Revealing
Lectin -
sugar Interactions with a Single
Au@
Ag Nanocube . -
pubmed
An individual nanoparticle - based plasmonic nanotechnology was used for real - time revealing
lectin -
sugar interactions , which could be designed as novel plasmonic nanobiosensors for detection of trace ConA with high sensitivity and selectivity . -
pubmed
In fact , each
Au@
Ag NC can act as a nanobiosensor for quantified detection of trace ConA , which miniaturized the biosensor system to nanoscale . -
pubmed
We expect that this nanobiosensor system can provide alternative important method for the monitoring the
lectin -
sugar specific binding at a single nanoparticle surface . -
pubmed
Treatment with
benzodiazepines , several previous
psychiatric admissions , and the absence of improvement after
ECT were associated with
relapse . -
pubmed
Peripheral ALCSF was statistically lower for both addition powers of the MFCLs when compared to SVCLs ( 12.70 for
Add2 and
Add4 , 13.73 for SVCLs , p = 0.009 ) . -
pubmed
INTRODUCTION : Once - weekly
semaglutide has been associated with greater reductions in
glycated hemoglobin ( HbA1c ) and
body weight than
sitagliptin and dulaglutide in the SUSTAIN 2 and 7 clinical trials , respectively . -
pubmed
IL-18 expression , cell proliferation , and apoptosis were examined using Western blotting ,
methylthiazolyldiphenyl - tetrazolium bromide assay , and flow cytometry , respectively . -
pubmed
Further experiments to clarify the
MOF 589 activity indicated that the
BIPA - TC linker might have an important impact through a cooperative effect on the metal cluster . -
pubmed
KEGG enrichment analysis found that
cortisol regulated LPS - stimulated immune responses mainly focus on
cytokines , complement and coagulation cascades pathways , antigen presentation pathways , haematopoiesis , and
inflammation . -
pubmed
METHODS : Twenty - eight well - trained swimmers completed an incremental test for maximal
oxygen uptake ( Peak-[Formula : see text ] ) and maximal aerobic velocity (
MAV ) assessment . -
pubmed
LncRNA
HOTAIR - mediated
MTHFR methylation inhibits
5 - fluorouracil sensitivity in
esophageal cancer cells . -
pubmed
Interference with lncRNA
HOTAIR enhanced
5 - FU - induced apoptosis , exhibited anti - proliferative activity , and reduced promoter methylation of the
MTHFR in
EC cells . -
pubmed
CONCLUSION : Together , our study proposes that pharmacologic targeting of lncRNA
HOTAIR sensitizes
EC cells to
5 - FU - based chemotherapy by attenuating the promoter hypermethylation of the
MTHFR in
EC . -
pubmed
It was determined that the detection power of
GC -
MS system was improved 7.8 times for
bixafen and 119 times for
fipronil over LOD comparisons of conventional
GC -
MS and B - DLLME -
GC -
MS systems . -
pubmed
Meanwhile , development of therapeutic resistance to
trastuzumab , a common
breast cancer treatment administered after chemotherapy , is a significant challenge in the treatment of
HER2 - positive
breast cancer . -
pubmed
We , therefore , analyzed the role of
YAP in
trastuzumab resistance in
HER2 - positive -
breast carcinoma cells in vitro and evaluated the status of
YAP and related proteins in patient - derived
breast carcinoma tissues by immunohistochemistry . -
pubmed
In both
paclitaxel ,
doxorubicin and
cyclophosphamide (
TAC)-treated , and
docetaxel ,
carboplatin , and
trastuzumab ( TCbH)-treated groups ; the pathological complete response ( pCR ) ratios were inversely correlated with p -
AKT status in biopsy specimens , while
YAP and
p73 status were positively correlated with the pCR ratio in the biopsy specimens of the TCbH group . -
pubmed
Curcumol inhibits
KLF5 - dependent angiogenesis by blocking the ROS /
ERK signaling in liver sinusoidal endothelial cells . -
pubmed
Interestingly , this process may depend on
curcumol 's inhibition of the expression of transcription factor
KLF5 . -
pubmed
More importantly , we proved that
curcumol could suppress
KLF5 - mediated LSEC angiogenesis by inhibiting ROS /
ERK signaling . -
pubmed
SIGNIFICANCE : We suggested that transcription factor
KLF5 could be considered as a new target molecule of
curcumol in improving
liver fibrosis , and pointed out that
curcumol targeted ROS /
ERK - mediated
KLF5 expression could inhibit LSEC angiogenesis . -
pubmed
In this prospective trial , a pan - genotypic
PrEP with
daclatasvir and
sofosbuvir once daily for 12 week was administered at transplantation . -
pubmed
Moreover , the
alkaline phosphatase (
ALP ) activity at day 7 and the semiquantitative analysis of
Alizarin red staining at day 21 demonstrated that osteogenic differentiation of hBMSCs was enhanced in the
Ta group . -
pubmed
Here , we report a highly efficient catalyst in acid , that is , solid - solution
Ru -
Ir nanosized - coral (
RuIr -
NC ) consisting of 3 nm - thick sheets with only 6 at.%
Ir . -
pubmed
Among OER catalysts ,
RuIr -
NC shows the highest intrinsic activity and stability . -
pubmed
In addition to an anaerobic environment , maximum
enzyme synthesis was dependent upon the presence of a complete complement of
amino acids , with omission of
L - threonine ,
L - valine , or
L - leucine producing the largest decreases in
enzyme formation . -
pubmed
Half - maximal stimulation of
enzyme synthesis occurred with 0.4 mM
threonine in the medium . -
pubmed
The roles of anaerobiosis and
amino acids are interpreted as being in accord with the concept that
threonine dehydratase functions in anaerobic energy production under conditions of
amino acid sufficiency . -
pubmed
In the present study , using flow cytometric analysis of the binding of
merocyanine 540 to monitor transbilayer phospholipid distribution ,
Ca ( 2 +)-induced loss of asymmetry is shown to be independent from the
aminophospholipid translocase which catalyzes movement of normally internal phospholipids from the outer to the inner leaflet of the membrane . -
pubmed
Addition of
ATP during lysis reverses loss of asymmetry , and this restoration can be blocked by inhibitors of the
aminophospholipid translocase . -
pubmed
The interplay of purified
eIF-4A , - 4B , and - 4F was studied in relation to
ATP dependence and cap analogue sensitivity of cap binding . -
pubmed
The interaction of
eIF-2 with the 5 ' end of mRNA was extremely sensitive to K(+)-ions and was resistant to a high concentration of
Mg ( 2 +)-ions ; this influence of mono- and divalent ions was in contrast with the cross - linking of
eIF-4B and - 4E. -
pubmed
Effects of
dopamine receptor antagonists on the inhibition of the spike generation by conditioning stimuli applied to the ventral tegmental area preceding the test stimulus to the parafascicular nucleus and those of
dopamine agonists on the test stimulus - induced spikes were examined . -
pubmed
For the
rest of the day till the following morning after the test the blood
sugar was elevated , as compared with the control level of the 4th day . -
pubmed
Neutrophil elastase activity and
superoxide production are diminished in neutrophils of alcoholics . -
pubmed
The aim of this study was to examine the effect of
ethanol consumption on
neutrophil elastase activity and
superoxide production of peripheral blood neutrophils . -
pubmed
After 6 to 10 days ,
elastase activity and
superoxide production were significantly greater than they were at Day 0 and approached normal levels . -
pubmed
However , the
type IIIb enzyme has a Ki for
trimethoprim of 0.4 microM , and a pI of 5.35 ( as compared to 19 nM and 6.1 for the type IIIa ) ; both these results suggest that it is a different enzyme from the prototype type IIIa . -
pubmed
The
type IIIb dihydrofolate reductase was purified by
methotrexate agarose affinity chromatography , yielding a pure protein as determined by HPLC . -
pubmed
Automatic
amino acid analysis of the purified enzyme showed it to be distinct from all other known plasmid - encoded
dihydrofolate reductases and quite different from the
type IIIa enzyme . -
pubmed
The purified
enzyme was examined by
SDS - PAGE , which revealed that the
type IIIb dihydrofolate reductase was a monomeric protein of Mr 17,200 . -
pubmed
No isotope effect ( 1.00 + /- 0.03 ) was observed in the
5 - lipoxygenase reaction for conversion of the initially formed product
5 - hydroperoxyeicosatetraenoic acid to the
epoxide leukotriene A4 . -
pubmed
Correlation of some published
amino acid sequences for
photosystem I polypeptides to a 17 kDa
LHCI pigment - protein and to subunits III and
IV of the core complex . -
pubmed
237 , 108 - 112 ] is not that of subunit
IV but rather that of the 17 kDA
LHCIc pigment protein . -
pubmed
14 , 511 - 518 ] is indeed that of subunit III ; seemingly conflicting identifications , based on apparent sizes on
SDS - PAGE , of which polypeptides are subunits III and
IV are probably explained by subunit III 's electrophoretic migration rate being dependent on the
solvent . -
pubmed
The administration of
DISO prior to overdrive atrial pacing improves the rate of conversion to
SR and allows an easier stimulation protocol with a lower incidence of pacing - induced
atrial fibrillation . -
pubmed
Since it has been demonstrated that the use of opiates during pregnancy alters
placental receptors , the effects of
cocaine on
placental receptors was studied . -
pubmed
This study investigated the roles of cholinergic reflex stimulation and decreased
beta 2 - adrenoceptor responsiveness to explain
adenosine 's bronchoconstrictor action . -
pubmed
The IB increased FEV1 by 11 to 15 % and SGaw by 69 to 73 % and provided a large degree of protection against
methacholine , with a geometric mean concentration ratio (
CR ) of 196 when airway caliber was measured as SGaw ( p less than 0.001 ) . -
pubmed
In contrast , IB provided little protection against
adenosine - induced
bronchoconstriction (
CR 1.3 for SGaw and 1.51 for FEV1 ) . -
pubmed
Various
calmodulin antagonists such as
chlorpromazine ,
trifluoperazine and
N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalenesulfonamide , not only activated the three reactions but also greatly enhanced their sensitivity to
Ca2 + ( K0.5 , 0.1 - 0.3 microM ) . -
pubmed
Although platelet membranes contained endogenous
calmodulin ( 0.3 - 0.6 microgram / mg of membrane protein ) , the addition of exogenous
calmodulin inhibited
choline exchange activity but had no or little effect on
serine or
ethanolamine exchange activity . -
pubmed
The results suggest that in the presence of low
Ca2 + concentrations , these drugs markedly stimulate base - exchange activities , and
choline exchange activity may be regulated by
calmodulin . -
pubmed
There was a significant fall in basal levels of
gastrin ( -39 % , P less than 0.001 ) ;
gastric inhibitory peptide ( -44.7 % , P less than 0.001 ) ; enteroglucagon ( -25 % , P less than 0.001 ) and
neurotensin ( -33 % , P less than 0.05 ) . -
pubmed
There was no significant change in plasma levels of
insulin ,
motilin ,
pancreatic polypeptide or
glucose . -
pubmed
Specific immunoprecipitates of
aminopeptidase M , an established transmembrane protein , and of
gamma - glutamyltransferase were isolated from vesicles solubilized with
Triton X-100 or with
papain . -
pubmed
With the samples derived from internally labeled vesicles , the
triton - solubilized form of each enzyme was intensely labeled , whereas the
papain - solubilized forms contained insignificant amounts of radioactivity . -
pubmed
No gradient was recorded at
rest , but it could be provoked by
isoproterenol . -
pubmed
The levels of all other
amino acids in blood , urine , and
CSF were within the normal range . -
pubmed
Since
aspartic acid is a putative excitatory neurotransmitter , a possible causal relationship is suggested between its increased
CSF concentration and the occurrence of
neonatal convulsions in this family . -
pubmed
After introduction of screening for
alanine aminotransferase and antibodies to
hepatitis B core antigen the risk of seroconversion was 4 % per patient ( 0.26 % per unit ) . -
pubmed
CONCLUSION : These results suggest that modification of the treatment regimen is needed , with higher doses of
clarithromycin and combination therapy with
proton - pump inhibition . -
pubmed
RSC - b and - c were found to be homologous proteins having similar
amino acid compositions and N - terminal sequences . -
pubmed
These
enzymes are stable at pH 4 - 8 and their optimum pHs toward
glycolchitin are 5 . -
pubmed
Superoxide dismutase activities and mRNA levels were not affected by
hyperoxia , whereas
catalase activity was slightly decreased after 24 h in 80 %
oxygen at Day 17 . -
pubmed
The presence of
mGluR1 alpha in cells of the hippocampus that are associated with the release of
somatostatin , suggest that this receptor could play a role in regulating hippocampal excitability in both normal and
epileptic tissues . -
pubmed
The evidence suggests that the clones contain DNA sequences encoding functions required for
geldanamycin biosynthesis including components of the
polyketide synthase . -
pubmed
The activities of
dephosphophosphorylase kinase and
phosphorylase a were stimulated by each hormone or
Bt2cAMP . -
pubmed
Pretreatment of leaves with
cycloheximide does not inhibit the response to
auxin treatment , suggesting that hormone sensitivity of these promoter elements does not involve de novo synthesis of
ASF-1 . -
pubmed
Based on these results , we propose that transcriptional activation by
ASF-1 may be modulated by
auxin through modification of pre - existing factors . -
pubmed
Our results also suggest a role for
ASF-1 in mediating some of the effects of
auxin in vivo . -
pubmed
An increased sensitivity of glycolysis to
insulin measured in vitro by both
lactate production and
glucose transport was observed following the acute exercise . -
pubmed
This process was reversed by the addition of
2 - chloroadenosine , an
adenosine receptor agonist , to the incubation medium . -
pubmed
Our findings confirm earlier studies suggesting that improved
insulin sensitivity of
glucose utilization by skeletal muscles after endurance exercise may be at least partly explained by an alteration in the
adenosine concentration or changes in the properties of
adenosine receptors in soleus muscle . -
pubmed
Prolactin enables normal development of
ACh - stimulated current in cultured larval bullfrog skin . -
pubmed
ACh ,
carbamylcholine , and
choline stimulated SCC in skin that had been cultured with
aldosterone ( 5 x 10(-7 ) M ) supplemented with
prolactin (
PRL ; 2 micrograms / ml ) . -
pubmed
Eserine , an inhibitor of
acetylcholinesterase , also inhibited the
ACh response . -
pubmed
The main
tumour results were : ( a ) original
DS induced a higher
tumour rate than extracted
DS ; ( b ) the carcinogenic potency of extracted CB probably depends on the size of the primary
carbon particles and on the specific surface area of the particles ; ( c ) extracted
DS covered with 11 micrograms
BaP per mg
carbon particles caused a lower
lung tumour rate than original
DS containing only 0.9 ng
BaP per mg , but a variety of other
PAH and
NO2 - PAH ; ( d ) a total dose of 15 mg pure
BaP caused a
lung tumour rate very similar to that of 30 mg extracted
DS , 15 mg original
DS or 15 mg Printex 90 T CB extracted or covered with approximately 29.5 micrograms
BaP per mg CB . -
pubmed
Addition of
calmodulin ( 1 microM ) , or the
calmodulin inhibitors
mastoparan and
calmidazolium ( 5 microM ) , did not alter the action of
Ca2 + on the
cGMP - activated current . -
pubmed
We analysed
fatty acid compositions , in particular
arachidonate , and measured the amount and the activity of high molecular weight cytoplasmic
phospholipase A2 (
cPLA2 ) of these tissues . -
pubmed
cPLA2 was separated by
SDS - PAGE , Western - blotted , immunoblotted using a specific antibody to
cPLA2 and semiquantified following enhanced chemiluminescence using a scanning densitometer . -
pubmed
cPLA2 biological activity was also assayed using
1 - stearoyl , 2 -[ 1 - 14C ]- arachidonyl ,
L-3 - phosphatidylcholine . -
pubmed
Compared with
normal mucosa / submucosa , there was more total
arachidonate in
tumours ( P & lt ; 0.01 ) , and increased levels of
cPLA2 occurred in 6 of 17
tumours . -
pubmed
There is a
serine tRNA binding site downstream of the 5 ' LTR sequence and a polypurine tract upstream of the 3 ' LTR end . -
pubmed
When the highly purified
polyisoprenyl phosphate phosphatase was analyzed by
SDS - polyacrylamide gel electrophoresis , a major 33 - kDa polypeptide was observed . -
pubmed
Enzymatic dephosphorylation of
Dol - P by the purified
phosphatase was 1 ) optimal at pH 7 ; 2 ) potently inhibited by F- ,
orthovanadate , and
Zn2 + & gt ;
Co2 + & gt ;
Mn2 + but unaffected by
Mg2 + ; 3 ) exhibited an approximate Km for
C95 - Dol - P of 45 microM ; and 4 ) was sensitive to
N - ethylmaleimide ,
phenylglyoxal , and
diethylpyrocarbonate . -
pubmed
The
pig brain phosphatase did not dephosphorylate
glucose 6 - phosphate ,
mannose 6 - phosphate ,
5 '- AMP , or
p - nitrophenylphosphate , but it dephosphorylated
dioleoyl - phosphatidic acid at initial rates similar to those determined for
Dol - P. Based on the virtually identical sensitivity of
Dol - P and
phosphatidic acid dephosphorylation by the highly purified
enzyme to
N - ethylmaleimide ,
F - ,
phenylglyoxal , and
diethylpyrocarbonate , both substrates appear to be hydrolyzed by a single enzyme with an apparent dual specificity . -
pubmed
Although the
enzyme is
Mg2 + -independent and capable of dephosphorylating
Dol - P and
PA , several enzymological properties distinguish this
lipid phosphomonoesterase from
PAP2 . -
pubmed
The production and release of pro - inflammatory
cytokines are affected by
theophylline showing a potent inhibitory effect on the production of
IL-1beta ,
TNF - alpha and
IFN - gamma . -
pubmed
Nycthemeral significant variation in
factor VII when
acenocoumarol was administered once daily was noted ( P = 0.02 ) , but the clinical relevance of
factor VII variation at steady - state is uncertain . -
pubmed
The
CCl4 administration alone caused
hepatocellular necrosis , severe
anemia ,
leucopaenia ,
lymphocytopaenia ,
neutrophilia ,
eosinophilia and
haemoglobinaemia along with the alterations of
plasma albumin and
globulin . -
pubmed
These cardiovascular effects appear to involve complex interactions at the level of the peripheral
beta ( 1 )- , beta ( 2 )- , and alpha ( 2 )- adrenoreceptor - dependent as well as
M ( 2 )- and M ( 4 )- cholinergic receptor - dependent transductional pathways both in cardiovascular myocells and at the level of the postganglionic sympathetic endings ( with
reserpine- and
guanethidine - like mechanisms ) . -
pubmed
Thyroid peroxidase (
TPO ) as a
tumor marker in the follow - up of differentiated
thyroid carcinomas with surgical and ablative
radioiodine therapy . -
pubmed
In relation to the extension of residues , some cases presented increasing of
TPO and
hTG after
radioiodine treatment . -
pubmed
The clinical value of
TPO seems to be in the time being limited to
DTA due to false negative
hTg values . -
pubmed
An energy transfer acceptor was attached to
Cys374 of
actin and a donor to the
tropomyosin heterodimer at either
Cys36 of the beta - chain or
Cys190 of the alpha - chain . -
pubmed
ATP , which dissociates
myosin from
actin , completely reversed the changes in FRET induced by heads , whereas in the presence of
ADP the effect of heads was the same as in its absence . -
pubmed
The results indicate that
myosin with and without
ADP , intermediates in the
myosin ATPase hydrolytic pathway , are effective regulators of
tropomyosin position , which might play a role in the regulation of smooth muscle contraction . -
pubmed
Five of the anti -
CYP2D6 - positive sera recognized a peptide between
amino acids 200 and 429 including
amino acids 254 - 271 . -
pubmed
Androgens mediate their effects through the
androgen receptor (
AR ) and have antiproliferative effects on uterine endometrial cells . -
pubmed
Reverse transcription -
polymerase chain reaction was performed by using UEC cell lines with and without treatment by the demethylating reagent
5 - aza-2 '- deoxycytidine . -
pubmed
Treatment with
5 - aza-2 '- deoxycytidine restored
AR expression in all of the UEC cell lines that showed no
AR expression before treatment . -
pubmed
This study is the first to report that the possible mechanism of
AR inactivation in
endometrial cancer is through hypermethylation of the
AR gene
CpG islands . -
pubmed
This hyperthermophilic
enzyme did not show domain movement upon binding of an acidic substrate ,
glutamate , except for a small movement of the alpha - helix from
Glu16 to
Ala25 . -
pubmed
Fluorescent staining of
glycoproteins on
polyvinylidene difluoride membrane with
8 - aminonaphthalene-1,3,6 - trisulfonate . -
pubmed
Here , we describe a simple and sensitive method that allows fluorescent detection of
glycoproteins on
polyvinylidene difluoride (
PVDF ) membrane . -
pubmed
We used
periodic acid oxidation of
carbohydrate chains of
glycoproteins and fluorescent labeling with
8 - aminonaphthalene-1,3,6 - trisulfonate (
AN - TS ) by reductive amination . -
pubmed
There is compelling evidence that topical
corticosteroids down - regulate
cytokine expression , and it is likely that a larger , and longer multi - centre trial may prove their efficacy in
CRS . -
pubmed
The detrimental effects that
cytokines impart on metabolic function have been associated with
nitric oxide (
NO ) production and apoptosis . -
pubmed
RESULTS :
Cytokine - induced suppression of
glucose - stimulated
insulin release was enhanced in a time - dependent manner . -
pubmed
NO production was stimulated by
IL-1 beta and augmented by
TNFalpha and
IFN gamma . -
pubmed
N ( G )- Monomethyl - l - arginine (
l - NMMA ) blocked
cytokine - induced
NO production but only partially attenuated suppression of
glucose - stimulated
insulin release . -
pubmed
Apoptosis increased in the presence of
cytokines and was slightly reduced when
NO production was specifically inhibited . -
pubmed
Here , we extended this observation reporting that the mutation of
serine-46 of
Cdc28 to
glutamic acid doubles , at least in vitro , the
H1 - kinase activity of the
Cdc28 /
cyclin A complex . -
pubmed
Corticosteroid hormone - induced factor (
CHIF ) is a short epithelial - specific protein that is independently induced by
aldosterone and a high - K(+ ) diet . -
pubmed
A number of studies have suggested that these proteins are involved in the regulation of
ion transport and , in particular , functionally interact with the
Na (+)- K (+)- ATPase . -
pubmed
Upregulation of
VEGF mRNA by
hypoxia was mimicked by
CoCl2 and
desferrioxamine in normoxic AEC and was not prevented by inhibitors of reactive
oxygen species , suggesting that hypoxic
VEGF regulation involved an
O2 - dependent protein that requires
ferrous ions but is independent of reactive
oxygen species generation . -
pubmed
Cocaine - induced proliferation of dendritic spines in nucleus accumbens is dependent on the activity of
cyclin - dependent kinase-5 . -
pubmed
It has been shown previously that chronic
cocaine administration increases the expression of
cyclin - dependent kinase-5 in this brain region and that this
neuronal protein kinase regulates
cocaine - induced locomotor activity . -
pubmed
These data indicate that
cyclin - dependent kinase-5 is involved in regulation of , as well as
cocaine - induced changes in , dendritic spine density . -
pubmed
Mean arterial ( MAP ) and corpus cavernosal ( CCP ) pressures were monitored during intracavernosal injection of the
Rho - kinase inhibitor
Y-27632 . -
pubmed
Intense neural activity reduced glycogen content in the presence of 10 mM bath
glucose and
CAP area gradually declined . -
pubmed
CAP area declined more rapidly during high frequency stimulation if
monocarboxylate transport was inhibited . -
pubmed
The comparable decrease in activities was observed for the
ouabain - sensitive and
ouabain - resistant
Na + , K (+)- ATPase components ( isoform species ) . -
pubmed
Mg ( 2 +)- ATPase increases in postnatal period independent of
Na + , K (+)- ATPase activity and it remains insensitive to postnatal maternal
alcohol intake . -
pubmed
The decomposition of
hydrogen peroxide by
hemocyanin and by its dissociation products . -
pubmed
Sulfhydryl content and tryptic susceptibility of thermally denatured
ovalbumin . -
pubmed
[ Protein concentration ,
hemoglobin , hematocrit values and
oxygen content in maternal and umbilical cord blood ] . -
pubmed
The title
4,4 '- disubstituted diphenyl-1,3 - triazines ,
C14H15N3 , ( I ) ,
C12H9ClFN3 , (
II ) , and
C13H12FN3 , ( III ) , each contain a
triazene group
( - N = N - NH - ) having an extended conformation . -
pubmed
The dihedral angles between the two
benzene rings in ( I ) , (
II ) and ( III ) are 4.3 , 3.4 and 6.5 degrees , respectively . -
pubmed
Nitric oxide (
NO * ) is an effective chain - breaking antioxidant in free radical - mediated lipid oxidation (
LPO ) . -
pubmed
Using
oxygen consumption as a measure of
LPO , we simultaneously measured
nitric oxide and
oxygen concentrations with
NO * and
O2 electrodes . -
pubmed
Bolus addition of
NO * ( 1.5 microM ) inhibited
LPO when the
NO * concentration was greater than 50 nM. -
pubmed
Similarly , using
( Z )- 1 -[ N -( 3 - ammoniopropyl )- N -( n - propyl ) amino ] diazen-1 - ium-1,2 - diolate as a
NO * donor we found that an average steady - state
NO * concentration of at least 72 + /- 9 nM was required to blunt
LPO . -
pubmed
Erlectin (
XTP3 - B ) is member of a protein family containing
mannose 6 - phosphate receptor homology ( MRH- , or
PRKCSH- ) domains implicated in N - glycan binding . -
pubmed
Aldh null larvae and adults are poisoned by
ethanol concentrations easily tolerated by wild - types ; their
ethanol sensitivity is in fact comparable to that of
Adh nulls . -
pubmed
reinhardtii and the saturation of
ribulose-1,5 - bisphosphate carboxylase at low extracellular
carbon concentrations . -
pubmed
Since translatable RNA for the polypeptide responsible for
carbonic anhydrase activity was only present in cells that experienced low levels of
CO ( 2 ) , the adaptation process either involves the regulation of transcription of the
carbonic anhydrase gene ( and perhaps other genes involved in adaptation ) or the
post - transcriptional processing of the messenger RNA . -
pubmed
The isolates could be divided into six classes , namely
beta - lactamase - non - producing
ampicillin - susceptible ( 25.0 % ) ,
TEM-1 beta - lactamase - producing
ampicillin - resistant ( 11.0 % ) ,
beta - lactamase - non - producing low - level
ampicillin - resistant with N526 K or R517H substitution in the
ftsI gene ( 30.4 % ) , BLNAR with an S385 T substitution together with either N526 K or R517H substitution in
ftsI ( 22.2 % ) , BLPACR - I with either a N526 K or R517H substitution in
ftsI ( 9.5 % ) and BLPACR -
II with an S385 T substitution together with either a N526 K or R517H substitution in
ftsI ( 1.9 % ) . -
pubmed
Using
CYP supersomes and liver microsomes , it was shown that
aplidine was metabolised mainly by
CYP3A4 and also by
CYP2A6 ,
2E1 and
4A11 . -
pubmed
However , the
aplidine metabolites formed by
CYP were further conjugated by the phase
II enzymes
UGT ,
GST and
SULT . -
pubmed
These results provide evidence that
CYP3A4 has a major role in metabolising
aplidine in vitro with additional involvement of
CYP2A6 ,
2E1 , and
4A11 . -
pubmed
CoCl ( 2 ) microinjected in the vMPFC significantly reduced freezing and attenuated MAP and
HR increase of the conditioned group . -
pubmed
However
sulphur treatment is not without its risks ; a
sulphur spring dermatitis has been described from a spa bath in Taiwan . -
pubmed
Carbon - ion radiotherapy for locally advanced or unfavorably located
choroidal melanoma : a Phase I /
II dose - escalation study . -
pubmed
Following the insult , synaptic activity was examined by recording orthodromically evoked
CA1 subfield responses , while mitochondrial activity was assessed by spectrophotometric measurement of
formazan produced by metabolism of
2,3,5 - triphenyltetrazolium chloride . -
pubmed
Inhibition of the 26 S proteasome with
MG132 rescued
cyclin D1 protein levels , indicating that rather than inhibiting translation ,
PKR and
PERK act to increase
cyclin D1 degradation . -
pubmed
Interestingly , this effect still requires
eIF2alpha phosphorylation at
serine 51 , as
cyclin D1 remains unaffected in cells containing a non - phosphorylatable form of the protein . -
pubmed
Furthermore , this degradation is independent of phosphorylation of
cyclin D1 at
threonine 286 , which is mediated by the
glycogen synthase kinase 3beta and
mitogen - activated protein kinase pathways as described in previous studies . -
pubmed
These data show that the administration of
epibatidine induces excitation of locus coeruleus neurons , which is mediated by
nicotinic receptors . -
pubmed
Identification of downstream targets of
estrogen and
c - myc in
breast cancer cells . -
pubmed
The
lipase showed high preference for the
S (+)- enantiomer of ibuprofen , which was esterified to the corresponding
S (+)- ibuprofen ester . -
pubmed
The
enzyme activities of
n - alcohols with different chain lengths ( 3 - 12 ) were compared , and it appeared that short- (
propanol and
butanol ) and long - chained (
decanol and
dodecanol )
alcohols were better substrates than the intermediate ones (
pentanol ,
hexanol , and
octanol ) . -
pubmed
However , unlike
secondary and tertiary alcohols , all of the tested
primary alcohols were substrates for the
lipase . -
pubmed
, the hydrolysis of
racemic ibuprofen ester in the microemulsion ) was also carried out enantioselectively by the
enzyme . -
pubmed
The high enantioselectivity displayed by the
lipase in the microemulsion system was seen neither in a similar esterification reaction in a pure organic solvent system (
isooctane ) nor in the hydrolysis reaction in an aqueous system ( buffer ) . -
pubmed
The residues in
FUS that are phosphorylated in vitro and in vivo were identified , and
phospho - specific antibodies were generated to demonstrate that
FUS becomes phosphorylated at
Ser(42 ) in vivo , primarily in response to agents that cause DSBs ( double - strand breaks ) . -
pubmed
DSB - induced
FUS phosphorylation in vivo at
Ser(42 ) requires
ATM and not
DNA - PK . -
pubmed
Although
Ser(42 ) is retained in the oncogenic
FUS -
CHOP [
C / EBP (
CCAAT / enhancer - binding protein )- homologous protein 10 ] fusion generated by a t(12;16)(q13;p11 )
chromosomal translocation ,
Ser(42 ) in
FUS -
CHOP is not phosphorylated after DNA damage . -
pubmed
Post - translational modification of
histone N - tails affects eukaryotic gene activity . -
pubmed
The treatment of
pertussis and
pneumonia complicating
pertussis ; the role of
hyperimmune gamma globulin and
sulfadiazine . -
pubmed
Carbonyl Reductase 3 (
CBR3 ) Mediates
9 - cis - Retinoic Acid - Induced
Cytostatis and is a Potential Prognostic Marker for
Oral Malignancy . -
pubmed
Our results suggest that the cytostatic effects of
RA could be mediated by the activation of endogenous
CBR3 gene in
oral squamous cell carcinomas (
OSCCs ) , and the expression is a potential marker for
oral malignancy . -
pubmed
Melatonin transmits photoperiodic signals through the
MT1 melatonin receptor . -
pubmed
All data suggest that the
melatonin signal that transmits photoperiodic information to the hypothalamo - hypophysial axis acts on the
MT1 receptor . -
pubmed
Activated pancreatic stellate cells (
PSC ) play a central role in the pathogenesis of
pancreatic fibrosis , a common feature of
chronic pancreatitis which is often caused by excessive
alcohol consumption . -
pubmed
In view of the central role of
connective tissue growth factor (
CCN2 ) in
fibrosis , we investigated the mechanisms by which
CCN2 is regulated in
PSC following their exposure to
ethanol or
acetaldehyde . -
pubmed
CCN2 production , assessed by reverse - transcriptase
polymerase chain reaction to measure
CCN2 mRNA levels or by fluorescence activated cell sorting to assess
CCN2 protein , was enhanced in a dose - dependent manner by
ethanol or
acetaldehyde . -
pubmed
In the presence of
4MP ,
DAS , or the anti - oxidants
vitamin D or
catalase , there was a substantial decrease in the ability of
ethanol to stimulate
CCN2 mRNA expression and a concomitant decrease in
CCN2 - positive
PSC . -
pubmed
Accumulation of reactive
oxygen species in
PSC after exposure to
ethanol was verified by loading the cells with
dichlorofluorescin diacetate and showing that there was a stimulation of its oxidized fluorescent product , the latter of which was diminished in the presence of
catalase or
vitamin D. -
pubmed
The potential therapeutic effects of inhibitors of
ethanol metabolism or anti - oxidants in
alcoholic pancreatitis may arise in part through their ability to attenuate
CCN2 production by
PSC . -
pubmed
The incretins and beta - cell health : contrasting
glucose - dependent insulinotropic polypeptide and
glucagon - like peptide-1 as a path to understand islet function in
diabetes . -
pubmed
None of the oils showed a
hydroxyl radical scavenging capacity & gt ; 50 % , but they showed an ability to inhibit
5 - lipoxygenase . -
pubmed
Pilot comparison of
F - fluorocholine and
F - fluorodeoxyglucose PET /
CT with conventional imaging in
prostate cancer . -
pubmed
Positron emission tomography / computed tomography ( PET /
CT ) with
( 18 ) F - flurodeoxyglucose (
FDG ) is also often suboptimal because of low tracer avidity , but
( 18 ) F - fluorocholine (
FCH ) appears to be a promising alternative molecular imaging probe . -
pubmed
RESULTS : Based on all imaging - detected disease sites , both FCH - PET /
CT and
FDG - PET /
CT ( 79 % ) were more sensitive than CI ( 14 % ) , with the highest number of sites of
nodal and distant disease on FCH PET /
CT . -
pubmed
Neonicotinoid insecticides control crop pests based on their action as agonists at the insect
nicotinic acetylcholine receptor , which accepts
chloropyridinyl- and
chlorothiazolyl - analogs almost equally well . -
pubmed
These
neonicotinoids effect a similar global transcriptional response to that of
SA , including genes involved in ( a)biotic stress response . -
pubmed
Furthermore , similar to
SA ,
IMI and
CLO induce systemic acquired resistance , resulting in reduced growth of a powdery mildew pathogen . -
pubmed
The action of
CLO induces the endogenous synthesis of
SA via the
SA biosynthetic enzyme
ICS1 , with
ICS1 required for
CLO - induced accumulation of
SA , expression of the
SA marker
PR1 , and fully enhanced resistance to powdery mildew . -
pubmed
In contrast , the action of
IMI does not induce endogenous synthesis of
SA . -
pubmed
Instead ,
IMI is further bioactivated to
6 - chloro-2 - hydroxypyridinyl-3 - carboxylic acid , which is shown here to be a potent inducer of
PR1 and inhibitor of
SA - sensitive enzymes . -
pubmed
Thus , via different mechanisms , these
chloropyridinyl- and
chlorothiazolyl - neonicotinoids induce
SA responses associated with enhanced stress tolerance . -
pubmed
METHODS : PCR was applied to obtain
Flavin - binding monooxygenase genes , then quantitative real - time
polymerase chain reaction ( Q - RT - PCR ) and RT - PCR were applied to analyze gene expression in response to different
LC - alkanes and
pristane . -
pubmed
Enhanced expression of
almA1 genes was observed when
strain A-11 - 3 grew with long chain
alkanes ( C28 to
C32 ) , in
sodium acetate medium . -
pubmed
Similarly ,
almA2 was induced by long chain
alkanes ( C24 to
C34 ) . -
pubmed
CONCLUSION :
AlmA genes were mostly responsible for the degradation of long - chain
alkanes and
pristane in
strain A-11 - 3 . -
pubmed
In addition ,
Thy-1 triggering enhanced
cytokine secretion by iNKT cells that were concomitantly stimulated with
alpha - galactosylceramide ( alphaGC ) , consistent with a co - stimulatory role for
Thy-1 in iNKT cell activation . -
pubmed
A 30 - mer phage displaying peptide library was constructed and screened against immobilized host cells , in which each peptide was overlapped 10aa to another peptide and spanned all
amino acid sequences of
Erns and
E2 . -
pubmed
Amino acid sequence analyses for two phage clones (
P2 and P6 ) demonstrated the strongest binding positions were at 101 - 130 ( S2 ) in
Erns , and 141 - 170 (
S6 ) in
E2 , respectively . -
pubmed
The mechanisms by which
thyroid stimulating hormone (
TSH ) regulates the expression and activity of
sodium iodide symporter (
NIS ) through
cAMP -
PKA have been partially elucidated by many studies . -
pubmed
In this study , we investigated the effects of the
TSH - mediated
cAMP -
PKA and
PLC -
IP3 pathways on the expression of
NIS in the presence of various intracellular iodinated compounds . -
pubmed
A high concentration of
iodine increased
NIS expression at the mRNA and protein levels ; however , this phenomenon was not observed in the presence of
MMI . -
pubmed
Both the
cAMP -
PKA and
PLC -
IP3 signaling pathways inhibited the expression of
NIS at low
iodine concentrations ; however , in thyrocytes treated with high concentrations of
iodine , the effect of
cAMP -
PKA on the expression of
NIS changed from inhibition to promotion , while the
PLC -
IP3 pathway continued to inhibit
NIS expression . -
pubmed
These findings indicate that intracellular iodinated compounds affect
NIS expression through the
TSH - mediated
cAMP -
PKA and
PLC - IP3 pathways . -
pubmed
Synthesis of fluorescently labelled
rhamnosides : probes for the evaluation of
rhamnogalacturonan II biosynthetic enzymes . -
pubmed
Three fluorescently labelled
saccharides 10 - 12 , representing structures found in pectic glycan rhamnogalacturonan
II ( RG -
II ) , were synthesised by chemical glycosylation of O-6 of
diacetone - d - galactose followed by deprotection and reductive amination with
amino - substituted fluorophore
APTS . -
pubmed
APTS - labelled glycans prepared in this manner were purified by
carbohydrate gel electrophoresis and subjected to capillary electrophoresis analysis , as a basis for the subsequent development of high sensitivity assays for
RG - II - active enzymes . -
pubmed
Divergent expression at the RNA and protein level was observed for the metabolic genes
pckA and
metE , involved in gluconeogenesis and
methionine synthesis , respectively . -
pubmed
Among them , several compounds displayed promising
seizure protection in the maximal electroshock
seizure (
MES ) , subcutaneous
metrazol seizure ( scMET ) , 6 Hz and
pilocarpine - induced status prevention ( PISP ) tests , with ED(50 ) values comparable to the reference anticonvulsant drugs ( AEDs ) . -
pubmed
Comparative LC -
MS studies on the leaf extract showed isomers of
O - caffeoylquinic acid (
3 - CQA ,
4 - CQA and
5 - CQA ) ,
O - feruloylquinic acid (
3 - FQA ,
4 - FQA and
5 - FQA ) and
para - coumaroylquinic acid (
3 - pCoQA and
5 - pCoQA ) . -
pubmed
In this oxidative muscle ,
Vo ( 2 ) and
O ( 2 ) delivery were closely matched during the transition period from
rest to steady - state contractions . -
pubmed
In conjunction with our previous work showing that speeding
O ( 2 ) delivery did not alter
Vo ( 2 ) on - kinetics under similar conditions , it appears that spontaneously perfused skeletal muscle operates at the nexus of sufficient and insufficient
O ( 2 ) delivery in the transition from
rest to contractions . -
pubmed
Also , the renal
nitrotyrosine level and
Txnip mRNA expression were lower when
GLN was administered . -
pubmed
Examination of the mechanistic activity of
MMP9 revealed its capability to degrade the adhesion
molecule N - cadherin as well as the basement - membrane protein
laminin within or around NCC , respectively . -
pubmed
The increased conjugation conferred to the
TF dyes by the addition of the pi - conjugated pendant groups increases both their light - harvesting and photovoltaic energy conversion capability in comparison with
N749 . -
pubmed
This colocalisation is dependent upon an intact microtubule network as depolymerisation by
nocodazole treatment abolishes
starvation - induced
MAP1B recruitment to these vesicles . -
pubmed
Both
nana and
oxNANA display alterations in
carbohydrate content , and the extent of these changes varies depending on growth light intensity . -
pubmed
Moreover ,
nana and
oxNANA are defective in the expression of some genes implicated in
sugar metabolism and photosynthetic light harvesting . -
pubmed
These findings suggest that the
NANA aspartic protease has an important regulatory function in
chloroplasts that not only influences photosynthetic
carbon metabolism but also plastid and nuclear gene expression . -
pubmed
VESW was associated with an increased incidence of delayed graft function (
DGF ) when
steroids were withdrawn within three d
post - transplantation . -
pubmed
Addiction to cigarette smoking is a prevalent
neuropsychiatric disorder that stems from the biophysical and cellular actions of
nicotine on
nicotinic acetylcholine receptors (
nAChRs ) throughout the central nervous system . -
pubmed
Although whole - cell patch
clamp recordings from neurons in brain slices is routinely done by the skilled electrophysiologist , it is challenging to locally apply drugs such as
acetylcholine or
nicotine to the recorded cell within a brain slice . -
pubmed
After 2 years , the change in spine
BMD was greater in each of the
zoledronate groups than in the placebo group ; values are mean ( 95 % confidence interval [ CI ] ) difference versus placebo :
zoledronate 1 mg 4.4 % [ 2.7 % to 6.1 % ] ; 2.5 mg 5.5 % [ 3.9 % to 7.2 % ] ; 5 mg 5.3 % [ 3.8 % to 6.7 % ] , p & lt ; 0.001 for each dose ) . -
pubmed
Change in
BMD at the total hip was greater in each of the
zoledronate groups than the placebo group ( mean [ 95 % CI ] difference versus placebo :
zoledronate 1 mg 2.6 % [ 1.5 % to 3.7 % ] ; 2.5 mg 4.4 % [ 3.5 % to 5.3 % ] ; 5 mg 4.7 % [ 3.7 % to 5.7 % ] , p & lt ; 0.001 for each dose ) . -
pubmed
Each of the bone turnover markers ,
beta - C - terminal telopeptide of type I collagen (
beta - CTX ) and
procollagen type - I N - terminal propeptide (
P1NP ) , was lower in each of the 2.5 - mg and 5 - mg
zoledronate groups than the placebo group throughout the trial ( p & lt ; 0.001 versus placebo for each marker for each dose at each time point ) . -
pubmed
The potency was predicted for a large set of
cyclic amidine and
guanidine cores extracted from
beta - secretase (
BACE-1 ) inhibitors . -
pubmed
The aim of this study was to assess the toxicological risks arising from the coexistence of
polyethylene glycol coated single - walled
carbon nanotubes ( SWCNTs -
PEG ) and a known environmental contaminant :
4 - nonylphenol (
NP ) . -
pubmed
Although the results provided no unambiguous evidences of synergistic toxicity between SWCNTs -
PEG and
NP , some specific treatments with mixtures ( SWCNTs -
PEG+NP ) resulted in an unexpected combined toxicity in relation to the individual treatments . -
pubmed
Only in those cases the interaction between SWCNTs -
PEG and
NP could have a synergistic effect on the resulting toxicity . -
pubmed
Overall , the serum had a " protective effect " on cells against all treatments : SWCNTs -
PEG ,
NP or their mixtures ( SWCNTs -
PEG+NP ) . -
pubmed
To determine the normal range of
Hemoglobin and cutoff values in healthy adults of Southern India , blood samples were analyzed for parameters of RBC and
iron metabolism in 177 male and 203 female medical students . -
pubmed
In female students ,
hemoglobin , hematocrit and parameters of
iron metabolism were lower than American white females , except TIBC which was higher . -
pubmed
The plasma
glucose and
insulin levels were analyzed periodically for three hours during the OGTT examinations . -
pubmed
Impaired fasting
glucose (
IFG ) was defined as a plasma
glucose level of & gt;=100 mg / dL to & lt;126 mg / dL. -
pubmed
Diabetes was defined as a two - hour
post - load plasma
glucose level of & gt;=200 mg / dL. -
pubmed
BACKGROUND :
Monophosphoryl lipid A ( MPLA ) , a nontoxic
TLR4 ligand derived from lipopolysaccharide ( LPS ) , is used clinically as an adjuvant in
cancer ,
hepatitis , and
malaria vaccines and in allergen - specific immunotherapy . -
pubmed
This family - based study analyzed the links between single
nucleotide polymorphisms ( SNPs ) in either the
POMC or
PCSK1 genes and
obesity , as well as
obesity - related traits among obese Thai children and their families . -
pubmed
Participants with the homozygous variant genotype in rs155971 had significantly elevated
cholesterol and
low - density lipoprotein cholesterol (
LDL - C ) levels ( P = 0.011 , OR = 1.025 , 95%CI = 1.006 - 1.045 ; and P = 0.006 , OR = 1.030 , 95%CI = 1.009 - 1.053 , respectively ) after adjustment for age , gender , and
body mass index (
BMI ) . -
pubmed
However , variants in
PCSK1 did affect
cholesterol level ,
LDL - C level , and waist circumference . -
pubmed
Assessment of a panel of
interleukin-8 reporter lung epithelial cell lines to monitor the pro - inflammatory response following
zinc oxide nanoparticle exposure under different cell culture conditions . -
pubmed
In terms of
ZnO - NP - induced
cytotoxicity pIL8 - RFP cells were the most affected , whilst the pIL8 -
Luc were found the least responsive . -
pubmed
Indeed , we observe that replication stress induced by the
CHK1 inhibitor
AZD7762 results in replication catastrophe and apoptosis , when combined with the
ATR inhibitor
VE-821 specifically in
cancer cells . -
pubmed
The
RD of
NDP was 90 mg / m(2 ) during cycles 1 - 3 . -
pubmed
Spectroscopic studies performed by NMR , UV - vis and
EPR , assisted by theoretical calculations , show that the
[ Cu ( no2th )]( 2 + ) complex presents a structure in solution similar to that observed in the solid state . -
pubmed
Association of Single
Nucleotide Polymorphisms in the Apoptosis - Related Genes
TP63 and
CD40 with Risk for
Lung Cancer in a Chinese Han Population . -
pubmed
Polyphenol coatings were applied to chitosan functionalized
polypropylene (
PP ) by
laccase assisted polymerization of
catechol and
catechin . -
pubmed
Polyphenol coated
PP exhibited both metal chelating ( 39.3 + - 2.5 nmol
Fe ( 3 + ) cm(-2 ) , pH 4.0 ) and radical scavenging ( up to 52.9 + - 1.8 nmol
Trolox eq cm(-2 ) ) capacity , resulting in dual antioxidant functionality to inhibit lipid oxidation and
lycopene degradation in emulsions . -
pubmed
Nonmigratory
polyphenol coated
PP inhibited
ferric iron promoted degradation better than soluble chelators , potentially by partitioning
iron from the emulsion droplet interface . -
pubmed
Evoked SPON excitation is comparatively weaker than evoked LSO excitation , likely due to a larger fraction of postsynaptic
GluR2 - containing
Ca2 + -impermeable
AMPA receptors after hearing onset . -
pubmed
AREAS COVERED : This review offers an explanation of the pharmacodynamics and of the pharmacokinetics of
ospemifene , and gives readers a complete overview of Phase
II and III studies on the clinical efficacy , tolerability and safety of this agent in the setting of
GSM . -
pubmed
Tumor cells do not express an enzyme called
arginosuccinate synthetase (
ASS ) and hence , these cells become auxotrophic for
arginine . -
pubmed
The
PARP Inhibitor
AZD2461 Provides Insights into the Role of
PARP3 Inhibition for Both Synthetic Lethality and Tolerability with Chemotherapy in Preclinical Models . -
pubmed
The
PARP inhibitor
AZD2461 was developed as a next - generation agent following
olaparib , the first
PARP inhibitor approved for
cancer therapy . -
pubmed
Here we demonstrate the efficacy of this compound against
olaparib - resistant
tumors that overexpress
P - glycoprotein . -
pubmed
Alkali and alkaline earth metal salts with
lithium ( 1 ) ,
sodium ( 2 ) ,
potassium ( 3 ) ,
rubidium ( 4 ) caesium ( 5 ) ,
magnesium ( 6 ) ,
calcium ( 7 ) ,
strontium ( 8) and
barium ( 9 ) were prepared and fully characterized using elemental analysis , IR and NMR spectroscopy , DSC and
TG analysis . -
pubmed
The four groups used in study were : 2 %
chlorhexidine digluconate , 2 %
doxycycline solution , 5 %
Proanthocyanidin (
PR ) , Control Group . -
pubmed
Amongst all the etching pretreatment groups , CHE group (
Chlorhexidine etching group ) revealed highest exposure to
collagen fibrils than the other groups of etching . -
pubmed
CONCLUSION : The
PDE4 inhibitor
roflumilast has a favorable side - effect profile at a cognitively effective dose and could be considered as a treatment in disorders affected by
disrupted sensory gating . -
pubmed
Involvement of
mitogen activated kinase kinase 7 intracellular signalling pathway in
Sunitinib - induced
cardiotoxicity . -
pubmed
The
tyrosine kinase inhibitor
Sunitinib is used to treat
cancer and is linked to severe adverse cardiovascular events . -
pubmed
Apoptosis signal - regulating kinase 1 (
ASK1 ) is the upstream activator of
MKK7 and is specifically inhibited by
2,7 - dihydro-2,7 - dioxo-3H - naphtho [ 1,2,3 - de ] quinoline-1 - carboxylic acid ethyl ester (
NQDI-1 ) . -
pubmed
This study investigates the role of
ASK1 ,
MKK7 and
JNK during
Sunitinib - induced
cardiotoxicity . -
pubmed
Administration of
Sunitinib ( 1 muM ) during Langendorff perfusion resulted in increased
infarct size , increased miR-133a expression , and decreased phosphorylation of the
ASK1 /
MKK7 /
JNK pathway compared to control . -
pubmed
Co - administration of
NQDI-1 ( 2.5 muM ) attenuated the increased
Sunitinib - induced
infarct size , reversed miR-133a expression and restored phosphorylated levels of
ASK1 /
MKK7 /
JNK . -
pubmed
These findings suggest that the
ASK1 /
MKK7 /
JNK intracellular signalling pathway is important in
Sunitinib - induced
cardiotoxicity . -
pubmed
Investigations into the
ASK1 /
MKK7 /
JNK transduction pathway could lead to development of cardioprotective adjunct therapy , which could prevent
Sunitinib - induced
cardiac injury . -
pubmed
Aerobic granular sludge was cultivated in 44 days (
R1 ) and 25 days (
R2 ) under the conditions of high settling velocity ( 18 m h-1 ) and high organic loading rate ( OLR ) ( 2.1 - 2.4 kg
COD m3 day ) . -
pubmed
The
Deubiquitinase OTU5 Regulates Root Responses to
Phosphate Starvation . -
pubmed
This divide and conquer strategy is first demonstrated in the fitting of PES for
OH3 with Gaussian process regression ( GPR ) and further applied to construct PESs for
CH5 and O+
CH4 with artificial neural networks (
NN ) . -
pubmed
Methods : Using experimental
DBS from the blood of an adult volunteer , we optimized extraction of small molecules and integrated measurement of
potassium as a proxy for blood hematocrit . -
pubmed
Normalizing for
potassium levels removed variability related to varying hematocrit across
DBS punches . -
pubmed
Aldosterone is synthesized in
zona glomerulosa of adrenal cortex in response to
angiotensin II . -
pubmed
The physiological significance of
Nurr1 in
aldosterone production in adrenal cortex has been well studied . -
pubmed
Furthermore ,
PARP1 inhibitor also suppressed the
aldosterone secretion in response to the
angiotensin II . -
pubmed
In addition , iodocyclization of
fluorinated propargylamine affords chiral
3 - iodo-2 -( trifluoromethyl )- 1,2 - dihydroquinoline , which can be easily converted to
2 -( trifluoromethyl )- 1,2 - dihydroquinoline derivatives with the selective
COX-2 inhibitory activity . -
pubmed
Simultaneous incubation with
verapamil showed that
P - glycoprotein does not play a relevant role on CA and KA oral absorption , with Papp of 4.48 ( + -0.26 ) x 10 - 6 cm / s and 5.37 ( + -0.72 ) x 10 - 6 cm / s observed for CA and KA , respectively . -
pubmed
B-0831 (
Ps3alphaHSD ) , which forms a dimer composed of two 26 kDa subunits , we expressed and purified
15N -
Tyr labeled
Ps3alphaHSD and its mutants , and analyzed the conformational change upon
NADH binding . -
pubmed
They were found to have low serum
phosphorus , normal
calcium , and
vitamin D studies , with elevated
alkaline phosphatase suggestive of
nutritional hypophosphatemia . -
pubmed
VraS is a
histidine kinase that undergoes autophosphorylation in the presence of signals elicited upon cell wall damage and it then transfers its
phosphoryl group to
VraR. -
pubmed
A single point mutation in the dimerization interface of
VraR , in which
Met13 was replaced by
Ala , led to the inability of
VraR to dimerize and to bind optimally to the target promoter . -
pubmed
The
BTD transition has also been suggested to be valid for
oxide glasses , but , unfortunately , direct prediction of Poisson 's ratio from chemical composition remains challenging . -
pubmed
TTP399 is a hepatoselective ,
glucokinase activator with potential for treating
type 2 diabetes . -
pubmed
In situ heating
TEM observations of evolving nanoscale
Al -
Mg -
Si -
Cu precipitates . -
pubmed
This research concerns the precipitation and subsequent dissolution of precipitate phases in an
Al-0.86Mg-0.62Si-0.1Cu ( at.% ) alloy subjected to varying thermal exposure during an in situ heating
TEM experiment . -
pubmed
CONCLUSION : The standard treatment of
hormone receptor positive and
HER-2 negative metastatic
breast cancer is
everolimus together with
exemestane . -
pubmed
In this scenario ,
inosine is a
purine nucleoside known to act as an agonist of
A1 and
A2A adenosine receptors . -
pubmed
Inosine pretreatment ( 25 mg / kg , oral route ) prevented the
hyperlocomotion induced by
ketamine ( 25 mg / kg , intraperitoneal route ) in the open - field test ; however , there was no difference in hippocampal density of
A1 and
A2A receptors , where
ketamine , as well as
inosine , were not able to promote changes in immunocontent of the
adenosine receptors . -
pubmed
In this sense , we suggest further investigation of signaling pathways involving
purinergic receptors , using pharmacological strategies to better elucidate the action mechanisms of
inosine on
bipolar disorder . -
pubmed
Specific cleavage of the recombinant proenzyme leads to the activation of
proPAO , followed by oxidation and oxygenation of
l - phenylalanine , resulting in an increase of chromogenic production when coupled with the Trinder reaction . -
pubmed
RESULTS : We applied this method to determine the activities of both
coagulation factor IIa and
plasmin in their physiologically relevant basal state and fully activated state in
sodium citrate - anticoagulated plasma respectively . -
pubmed
We aim to differentiate the effects of acute beta - adrenergic stimulation ( beta -
ARS ) and cardiomyocyte stretch ( increased sarcomere length ) on
calcium - transient dynamics and force generation , using a novel computational model of cardiac electromechanics . -
pubmed
An increase in beta -
ARS increased the amplitudes of force ( augmented inotropy ) and
calcium transient , and shortened both force and
calcium - transient duration ( lusitropy ) . -
pubmed
The
paracaspase mucosa - associated lymphoid tissue lymphoma translocation protein-1 (
MALT1 ) regulates
nuclear - factor - kappa - B (
NF - kappaB ) activation downstream of surface receptors with immunoreceptor
tyrosine - based activation motifs ( ITAMs ) , such as the
B - cell or T - cell receptor and has thus emerged as a therapeutic target for
autoimmune diseases . -
pubmed
Daily administration of the placebo solution ,
resveratrol ( 10 mg / kg ) , and ZLD ( 0.1 mg / kg ) was carried out for a period 42 days prior to initiation of
EP , and then for another 28 days following ligature placement . -
pubmed
IL-4 levels were significantly lower in the OVT + PLAC group versus OVT + ZLD +
RESV and SHAM + PLAC ( P < .05 ) . -
pubmed
NADPH oxidase (
nicotinamide adenine dinucleotide phosphate oxidase ) levels were significantly lower OVT +
RESV group when compared to OVT + PLAC ( P < .05 ) . -
pubmed
RESULTS : The addition of
DMOG resulted in
HIF-1alpha stabilization in the exterior of the engineered constructs , which resulted in increased regional
type II collagen deposition , but the stabilization did not translate to overall increased extracellular matrix deposition . -
pubmed
CONCLUSIONS : Despite previous studies that demonstrated the efficacy of
DMOG , here , short - term treatment with
DMOG did not have a uniformly positive impact on the chondrogenic capacity of
SYN - MSCs in either pellet culture or in scaffold - based engineered cartilage , as evidenced by reduced matrix production . -
pubmed
Galeterone sensitizes
breast cancer to chemotherapy via targeting
MNK /
eIF4E and
beta - catenin . -
pubmed
In our work ,
galeterone as a chemical compound under clinical trials for the treatment of
prostate cancer , was identified to be effective in targeting
breast cancer cells via suppressing
MNK -
eIF4E and
beta - catenin . -
pubmed
Mechanism analysis demonstrated that
galeterone decreased
MNK1 / 2 level and phosphorylation of
eIF4E. -
pubmed
In addition ,
galeterone decreased
beta - catenin level via promoting
GSK-3beta - mediated
beta - catenin degradation , and furthermore that
Akt but not
CK1 was involved in
beta - catenin degradation by
galeterone . -
pubmed
Rescue studies demonstrated that both
MNK /
eIF4E and
beta - catenin were responsible for anti -
breast cancer activity of
galeterone . -
pubmed
Loss and gain of function of
PDCD4 -
AS1 , miR-10b-5p or
IQGAP2 were performed before
MTT and colony formation assay , TUNEL staining in addition to Transwell and scratch assays were applied to measure the cell biological functions . -
pubmed
Stereospecific Response of E / Z - isomers of
N - Nitrososarcosine in LC - ESI -
MS /
MS . -
pubmed
Biochemical analyses of isolated Muller cells showed that ( a ) these cells did not synthesize and accumulate
acetylcholine ,
gamma - aminobutyric acid , or
catecholamines when incubated with appropriate radioactive precursors ; ( b ) the specific activities of
choline acetyltransferase (
EC 2.3.1.6 ) ,
glutamate decarboxylase (
EC 4.1.1.15 ) , and
tyrosine hydroxylase (
EC 1.14.16.2 ) in these cells were less than 2 percent of those found in the retina ; ( c ) Muller cells , however , contained high activities of transmitter degrading enzymes -
acetylcholinesterase (
EC 3.1.1.7 ) and
gamma - aminobutyrate - transamine (
EC 2.6.1.19 ) ; and ( d ) the cells also possessed high levels of two presumably glial - specific - enzymes -
glutamine synthetase (
EC 6.3.1.2 ) and
carbonic anhydrase (
EC 4.2.1.1 ) . -
pubmed
The findings suggest that alteration of the cell surface membrane and microfilaments prevents damage by interfering with cell - target interactions , that energy derived from glycolysis is required for
cytotoxicity , that cell - associated
esterases are probably involved , and that
cytotoxicity may be modulated by
cyclic nucleotides . -
pubmed
As more has become known about the mechanisms by which abnormal levels of circulating lipoproteins promote
atherosclerosis , certain risk factors have emerged as concerns for women , including : ( 1 )
diabetes mellitus as a risk factor , perhaps through its more profound effects on circulating lipoproteins ; ( 2 ) serum
triglyceride levels , and ( 3 ) changes in
high - density lipoprotein cholesterol . -
pubmed
This suggests that the high - affinity catalytic site presents a hydrophobic environment for
ATP binding in native
enzyme , that there is a significant difference between the conformation for
ADP binding as opposed to
ATP binding , and that the
ADP - binding conformation is more hydrophilic . -
pubmed
Cytosolic
diacylglycerol kinase was irreversibly inactivated by
5 '- AMP since the
enzyme remained less active after the removal of
5 '- AMP by P-10 gel chromatography . -
pubmed
The value of
penicillinase sensitive
penicillins in treating superficial
wound infections is questioned . -
pubmed
The
ATPase inhibitor
dicyclohexylcarbodiimide (
DCCD ) specifically inhibited
sulfate reduction but had no immediate influence on
sulfate accumulation . -
pubmed
The
K +- H +- antiporter nigericin ( in 150 mM
KCl ) and the
Na +- H +- antiporter monensin ( in 150 mM
NaCl ) caused partial inhibition of
sulfate accumulation , whereas the K+-transporter
valinomycin ( in 150 mM
KCl ) and the
Na+-H+ exchange inhibitor
amiloride ( 2 mM ) were without effect. -
pubmed
In contrast , postischemia treatment with
pentoxifylline was associated with significant recovery in
CIN and UFR , and stable RVR , RBF , and
RBP . -
pubmed
These findings offer further evidence for a primary role of
prolactin in lipid metabolism and indicate that
bromocriptine may be useful for treating
obesity and lipid - based
cardiovascular disorders . -
pubmed
Effects of prenatal exposure to
ethanol on neocortical development :
II . -
pubmed
Fetuses were administered with
[ 3H ] thymidine on
G13 ,
G17 , or
G20 and killed 45 - 60 minutes later . -
pubmed
TBPA was isolated by
retinol - binding protein (
RBP ) affinity chromatography and further purified by preparative
agarose gel electrophoresis or FPLC ion exchange chromatography . -
pubmed
TBG was purified by
thyroxine ( T4)-Sepharose chromatography followed by gel filtration on Sephacryl S-300 and preparative electrofocusing in a granulated
dextran gel . -
pubmed
Gel filtration of serum on
Sephacryl S-200 , identification of
T4 - binding proteins with 125I - T4 , and protein- and lipoprotein staining of fractions was performed . -
pubmed
Thyroxine - binding was found to
TBG in the
beta - globulin region ,
TBPA in the alpha 2 - region ,
albumin , and to the
high density lipoprotein (
HDL2 ) in the alpha 1 - region and the
very low density lipoprotein (
VLDL ) in the pre - beta region . -
pubmed
A corresponding band to the latter protein in serum was masked by
TBG and
TBPA , and T4 - binding in the alpha 1 - region was not always seen in serum . -
pubmed
It turned out that , if the first postirradiation cell division occurs at higher
KCl concentrations ( " rapid " postirradiation recovery is inhibited ) , the frequency of mitotic reciprocal recombination within the gene
ADE2 - centromere region decreases . -
pubmed
Keeping of irradiated cells ( in the
G1 phase of the cell division cycle ) in
water at 28 degrees C prior to plating on the selective
agar containing 1.5 M
KCl leads to smaller frequency of gene conversion
lys2 - 25 /
lys2 - 22 - ---Lys+ , as compared with that for the cells immediately plated on the selective
agar . -
pubmed
Methoctramine reveals heterogeneity of
M2 muscarinic receptors in longitudinal ileal smooth muscle membranes . -
pubmed
Methoctramine , an atrial selective
muscarinic receptor antagonist in functional studies was , however , able to partially discriminate between these two receptors in our binding studies . -
pubmed
The photomyoclonic reflex ( PMR ) , consisting of a one- or two - component blink reflex associated with the flash electroretinogram (
ERG ) , is sensitive to the acute effect of intravenously administered
diazepam . -
pubmed
Chromogranin represents a family of proteins that coexist with
catecholamines in the secretory vesicles of adrenal medulla cells . -
pubmed
Basal
plasma GH concentrations before
hGHRH-44 administration were not statistically different in the two experiments [
glucose experiment , 2.1 + /- 0.1 ( + /-
SE ) ng / ml ; water experiment , 2.6 + /- 0.6 ng / ml ] . -
pubmed
An examination of the
zinc requirement for the catalytic activity of
3 - phosphoglyceraldehyde dehydrogenase . -
pubmed
Detection of
epsilon ( gamma - glutamyl ) lysine crosslinks is not only necessary for establishing the importance of the
dipeptide as a
post - translational modification of proteins , but provides information as to the importance of the
transglutaminase enzyme in a biological system . -
pubmed
Quantitative estimation of
epsilon ( gamma - glutamyl ) lysine normally involves its further separation by ion - exchange chromatography and its
post - column detection following derivatisation with
ninhydrin . -
pubmed
If there is only moderate mucosal damage , a rather weak correlation is seen either for the
trehalose - load - test or for the
disaccharidase activities with morphological changes . -
pubmed
Both methods in comparison show a better correlation between
trehalase activity and morphology than between
trehalose - load - test and morphology . -
pubmed
Upon recirculation of the brain ,
phosphocreatine ,
ATP , and
lactate concentrations recovered to control values in the paramedian neocortex and
CA1 zone of hippocampus and to near - control values in the striatum . -
pubmed
The
phosphocreatine and
ATP concentrations then fell and the
lactate levels rose in the striatum after 6 - 24 h , and in the
CA1 zone of hippocampus after 24 - 72 h. The initial recovery and subsequent delayed changes in the
phosphocreatine ,
ATP , and
lactate concentrations in the striatum and hippocampus coincided with the onset and progression of morphological
injury in these brain regions . -
pubmed
It was shown to fractionate into at least 11 components that can be detected by
coomassie blue staining , a radioligand - receptor assay ( RRA ) , and four
hCG RIAs using antisera against the purified
hCG , its alpha- and beta - subunits , and the
hCG beta carboxyl - terminal peptide . -
pubmed
Thus ,
sialic acid alone and/or differences in subunit assembly seem to be responsible for the electrophoretic heterogeneity of highly purified
hCG . -
pubmed
We show that the translocation t(12;16)(q13 :
p11 ) in
malignant myxoid liposarcoma can be a fusion of the
CHOP dominant negative transcription factor gene with a novel gene ,
FUS , which can result in fusion of the
FUS glycine - rich protein with the whole
CHOP coding region . -
pubmed
The effect of
progesterone on
sulfate uptake was abolished by blocking either the
cAMP pathway or the genomic action of
progesterone and was independent of the
cAMP - activatable apical
chloride channel . -
pubmed
To clarify the role of
serotonin , the in vivo and in vitro effects of the selective
5 - HT2 receptor antagonist ,
DV-7028 , on
MCT - induced
pulmonary hypertension were studied . -
pubmed
After two months the mean blood
sugar level rose again insignificantly and an insignificant decline of
glycosylated haemoglobin occurred . -
pubmed
Nucleotide binding to the hydrophilic C - terminal domain of the
transporter associated with antigen processing (
TAP ) . -
pubmed
NBD -
TAP1 bound to C-8 -
ATP -
agarose and was specifically eluted with
ATP or
EDTA . -
pubmed
Photoaffinity labeling of NBD -
TAP1 with the
ATP analogues
8 - azido -[ gamma-32P ] ATP and
3 '- O -[( 4 - azido-3,5 -[ 125I ] diiodo-2 - hydroxybenzoyl )- beta - alanyl ]- ATP was specific . -
pubmed
ATP hydrolysis by
NBD - TAP1 was not detected . -
pubmed
The described experiments demonstrate
ATP binding to an isolated domain of the antigenic peptide transporter ,
TAP , and therefore support the theory of
ATP - dependent peptide translocation . -
pubmed
One was a rapidly activating and slowly inactivating type of outward rectifier ( named
IK ( FR ) for fast rectifier
potassium current ) , similar to a
potassium current described in cardiac muscle , and the other was a transient A - current ( IA ) . -
pubmed
The thermal stability of
ceruloplasmin , as studied by differential scanning calorimetry , was affected by
calcium but not by
magnesium . -
pubmed
The interaction of
ceruloplasmin with the chromatographic material used for its isolation ,
Sepharose 4B derivatized with
chloroethylamine , was weakened by
calcium . -
pubmed
Isolation of a recombinant intracellular
beta - galactosidase by
ammonium sulfate fractionation of cell homogenates . -
pubmed
As the
ammonium sulfate concentration increased , the
enzyme was precipitated preferentially and at 30 % salt saturation nearly all had been recovered in the precipitate . -
pubmed
A significant increase in plasma cord blood
cotinine was detected in Group
II , newborns whose mothers were active cigarette smokers throughout the pregnancy . -
pubmed
A genetic algorithm for designing gene family - specific
oligonucleotide sets used for hybridization : the
G protein - coupled receptor protein superfamily . -
pubmed
A set of 15 oligos between 7 and 14
bases in length has correctly identified 70 % of the
GPCR cDNA collection sequences with 0.02 % false positives . -
pubmed
The
tumor LR expression was immunohistochemically determined in paraffin - embedded sections using the
MLuC5 monoclonal antibody which recognizes the 67 - kDa
LR and the
avidin -
biotin immunoperoxidase method . -
pubmed
Oxidative bioactivation of the
lactol prodrug of a
lactone cyclooxygenase-2 inhibitor . -
pubmed
The
lactol derivative of a
lactone cyclooxygenase-2 inhibitor ( DFU ) was evaluated in vivo and in vitro for its potential suitability as a prodrug . -
pubmed
TSP2 - null fibroblasts grew as aggregates on both surfaces , and their attachment was further compromised on ox -
PDMS . -
pubmed
METHODS : Recombinant adenoviruses expressing a series of
C - CAM1 mutants were generated , such as AdCAMF488 ( mutated
C - CAM1 containing Tyr-488 --> ; Phe-488 ) , AdCAMH458 ( intracellular domain deletion mutant containing 458
amino acids ) , AdCAMG454 ( intracellular domain deletion mutant containing 454
amino acids ) , and AdCAMDeltaD1(
C - CAM1 mutant containing first
Ig domain deletion ) . -
pubmed
The
EP4 receptor antagonist
AH23848B (
[ 1alpha ( z ) , 2beta5alpha ]-(+/-)- 7 -[ 5 -[[( 1,1 '- biphenyl )- 4 - yl ] methoxy ]- 2 -( 4 - morph olinyl )- 3 - oxo - cyclopentyl ]- 4 - heptenoic acid ) ( 29 microM ) had no effect on concentration - effect curves to the
EP receptor agonists . -
pubmed
The mixed
prostanoid receptor antagonist
AH6809 (
6 - isopropoxy-9 - oxaxanthene-2 - carboxylic acid ) competitively antagonised
prostaglandin E2 with a pA2 of 5.6+/-0.2 . -
pubmed
AH6809 ( 42 microM ) antagonised
misoprostol ,
11 - deoxy prostaglandin E1 , and the
prostanoid DP receptor agonist
BW245C (
5 -( 6 - carboxyhexyl )- 1 -( 3 - cyclohexyl-3 - hydroxypropyl ) hydantoin ) with apparent pA2 values of 5.6+/-0.3 , 5.1+/-0.9 and 5.9+/-0.4 ( n = 4 ) , respectively , but was ineffective against the
IP receptor agonist
cicaprost ( n = 4 ) . -
pubmed
The
prostanoid DP receptor antagonist
BW A868C (
3 - benzyl-5 -( 6 - carboxyhexyl )- 1 -( 2 - cyclohexyl-2 - hydroxyethylamino ) h ydantoin ) ( 50 nM ) had no effect on responses to
prostaglandin E2 or
misoprostol . -
pubmed
The resulting
ubiquitin - protein conjugates are degraded by the 26S proteasome , an
ATP - dependent
protease . -
pubmed
Dimerization of
sterol regulatory element - binding protein 2 via the helix - loop - helix -
leucine zipper domain is a prerequisite for its nuclear localization mediated by
importin beta . -
pubmed
The
sterol regulatory element - binding protein 2 (
SREBP-2 ) , a transcription factor of the basic helix - loop - helix -
leucine zipper ( bHLH - Zip ) family , is synthesized in the form of a membrane - attached precursor molecule . -
pubmed
When cells are deprived of
sterols , a two - step proteolytic processing releases the transcriptionally active N - terminal segment of
SREBP-2 , thereby allowing it to enter the nucleus . -
pubmed
It was found that the active form of
SREBP-2 exists as a stable dimer in solution and that the substitution of
leucine residues for
alanine in the
leucine zipper motif disrupted the dimerization . -
pubmed
Activation of
p53 transcriptional activity requires
ATM 's kinase domain and multiple N - terminal
serine residues of
p53 . -
pubmed
Finally , single point - mutations of
serines 15 , 20 , 33 or 37 did not individually block the
ATM - dependent activation of
p53 transcriptional activity by
bleomycin . -
pubmed
However , double mutations of either
serines 15 and 20 or
serines 33 and 37 blocked the ability of
ATM to activate
p53 . -
pubmed
In addition , activation of
p53 by
ATM requires multiple
serine residues in
p53 's transactivation domain . -
pubmed
A survey was sent to programs to assess the processes in place to assist patients in managing
cholesterol and reaching a
low - density lipoprotein (
LDL - C ) goal of less than 100 mg / dL. -
pubmed
The Y238
DAAO mutants have spectral properties similar to those of the wild - type enzyme but the degree of stabilization of the
flavin semiquinone and the redox properties in the free form of Y238S are different . -
pubmed
The purpose of Part
II was to further establish concurrent validity of the PROBS-14 with known pediatric behavior measures (
TRF , CTRS ) and to ascertain if the PROBS-14 better predicts a
cocaine behavioral effect . -
pubmed
The PROBS instrument offers a promising brief measure of child problem behaviors with adequate concurrent and predictive validity , and which outperforms the
TRF and CTRS in discriminating prenatal
cocaine exposure status . -
pubmed
Selective
serotonin reuptake inhibitors were most commonly prescribed , followed by tricyclic antidepressants , atypical and third - generation antidepressants , and
monoamine oxidase inhibitors . -
pubmed
Using whole - cell patch
clamp experiments , a novel
chloride current was identified . -
pubmed
The inwardly rectifying
rClC-2 chloride current was activated by hyperpolarization but not by pH variation . -
pubmed
A different depolarization - activated outwardly rectifying
chloride current was activated only by hypotonic challenge and may correspond either to
rClC-3 or
rClC-6 . -
pubmed
Based on our molecular analysis of
rClC proteins , this new
chloride current does not correspond to
rClC-2 ,
rClC-3 ,
rClC-6 , or
rClC-7 channels . -
pubmed
Exposure to high concentrations of
glucose and
insulin results in
insulin resistance of metabolic target tissues , a characteristic feature of
type 2 diabetes . -
pubmed
High
glucose ( HG ) induced a significant decrease in
insulin - stimulated
glucose uptake [ tracer - determined disappearance rate ( Rd ) , control 41.2 + /- 1.7 vs. HG 32.4 + /- 1.9 mg . -
pubmed
The effect of
hyperventilation and
probanthine on isolated
RS - T segment and T - wave abnormalities . -
pubmed
[
Blood transaminases as indication of tolerance to antidiabetic
sulfanylurea ] . -
pubmed
CONCLUSION : Amongst the differentially - expressed genes correlating with
imatinib resistance , several suggest the activation of alternative pathway(s ) that maintain viability and growth independently of
Bcr -
Abl kinase activity . -
pubmed
The discovery and synthesis of novel
adenosine substituted
2,3 - dihydro-1H - isoindol-1 - ones : potent inhibitors of
poly ( ADP - ribose ) polymerase-1 (
PARP-1 ) . -
pubmed
We found that potent inhibition of
poly ( ADP - ribose ) polymerase-1 (
PARP-1 ) was achieved when
isoindolinone was linked to
adenosine by a spacer group of a specific length . -
pubmed
The removal of
raffinose was due to the high
alpha - galactosidase (
alpha - gal ) activities of these
lactic acid bacteria , which was highest at pH 5.5 ( 5.0 U / ml ) . -
pubmed
[ Effect of
caffeine on
amylases ] . -
pubmed
Control of
cytochrome c oxidase activity by
nitric oxide . -
pubmed
Over the past decade it was discovered that , over - and - above multiple regulatory functions ,
nitric oxide (
NO ) is responsible for the modulation of cell respiration by inhibiting
cytochrome c oxidase (
CcOX ) . -
pubmed
Silibinin down - regulated
PSA mRNA expression and
PSA secretion in conditioned medium under basal and
DHT ( 10(-8 ) M ) stimulated conditions , which was paralleled by
PDEF down - regulation . -
pubmed
DHT alone stimulated
PDEF and
PSA gene expression and
PSA secretion . -
pubmed
The down - regulation of basal as well as
DHT stimulated
PDEF and
PSA by
silibinin demonstrates the antiproliferative potential of this agent . -
pubmed
We show that doses of gamma - radiation ( IR ) as low as 0.25 Gy , which generate vast numbers of SSBs but only a few DSBs per cell ( & lt;8 ) , promptly activate
ATM kinase and induce the phosphorylation of the
ATM substrates
p53 -
Ser15 ,
Nbs1 -
Ser343 and
Chk2 -
Thr68 . -
pubmed
1 Gy ) , which cause
Chk2 autophosphorylation on
Thr387 ,
Chk2 - dependent accumulation of
p21waf1 and checkpoint arrest in the S phase . -
pubmed
Compared with the nonfluorinated parent compounds 1i-1 ,
fluorinated 1 -( naphth-2 - yl ) methylimidazoles 1a - h turned out to be potent inhibitors of
CYP17 and
CYP19 enzymes . -
pubmed
In order to elaborate the function of different types of
opioid receptors in regulating
melatonin synthesis , we used a selective
mu - opioid receptor agonist ,
Tyr -[ D - Ala(2 ) ,
N - methyl - phe(4 ) ,
glycol(5 ) ] (
DAMGO ) , a selective
delta - opioid receptor agonist ,
Enkephalin [
D - Pen(2 ) ,
D - Pen(5 ) ] , (
DPDPE ) and a selective
kappa - opioid receptor agonist ,
( ( + ) -( 5alpha , 7alpha , 8beta )- N - methyl - N -[ 7 - ( 1 - pyrrolidinyl )- 1 - oxaspiro [ 4,5 ] dec-8 - yl ]- benzene acetamide ) (
U69593 ) to investigate the activity of
N - acetyltransferase (
NAT ) activity and
melatonin secretion . -
pubmed
These stimulatory effects were blocked by
naloxone , an
opioid receptor antagonist . -
pubmed
However , the
kappa - opioid receptor agonist
U69593 was unable to alter either the activity of
NAT or the level of
melatonin . -
pubmed
This study reveals that the stimulatory effect of
opioid receptor on
melatonin synthesis is mediated via the activation of
adenylate cyclase system . -
pubmed
He was given the
gonadotropin - releasing hormone agonist
leuprolide acetate and the
androgen receptor antagonist
flutamide for the treatment of
prostate cancer . -
pubmed
After the second injection of
leuprolide acetate , fasting
glucose and
hemoglobin A1c (
HbA1c ) levels were found to be markedly elevated ( 22.8 mmol / L and 10.5 % , respectively ) . -
pubmed
After improvement of
hyperglycemia by
insulin treatment , their glycemic control remained good after treatment with
pioglitazone without use of
insulin . -
pubmed
Natural
rhodopsin contains an
11 - cis - retinylidene chromophore . -
pubmed
Adenosine deaminases that act on RNA (
ADARs ) catalyze the site - specific conversion of
adenosine to
inosine in primary mRNA transcripts , thereby affecting coding potential of mature mRNAs . -
pubmed
Structural determinants that define the
adenosine moieties for specific
ADARs - mediated deaminations are currently unknown . -
pubmed
Erlotinib (
Tarceva ,
OSI-774 ; Pfizer , Inc. ) is an orally - active , targeted inhibitor of the
epidermal growth factor receptor (
EGFR /
HER1 ) , which is part of a key regulatory pathway in
cancer . -
pubmed
They were placed on a 6 - week cycle regimen comprising
capecitabine ( X ; 2,000 mg / m2 / day in two divided doses for 2 weeks then 1 week
rest ) ,
idarubicin ( I ; 10 mg / m2 / day , days 1 , 3 and 5 ) and
cyclophosphamide ( C ; 100 mg / m2 / day for 2 weeks then 1 week
rest ) . -
pubmed
Molecular aspects of
gefitinib antiproliferative and pro - apoptotic effects in
PTEN - positive and
PTEN - negative
prostate cancer cell lines . -
pubmed
We reported that the
EGFR - selective
tyrosine kinase inhibitor
gefitinib (
ZD1839 ;
Iressa ) is able to induce growth inhibition , G(1 ) arrest and apoptosis in
PCa cells and that its effectiveness is associated primarily with
phosphatase and tensin homologue deleted from chromosome 10 (
PTEN ) expression ( and thus
Akt activity ) . -
pubmed
In fact
PTEN - negative
PCa cells are slowly sensitive to
gefitinib treatment , because this molecule is unable to downregulate
PI3K /
Akt activity . -
pubmed
PI3 K inhibition , by
LY294002 or after
PTEN transfection , restores
EGFR - stimulated
Akt signalling and sensitizes the cells to pro - apoptotic action of
gefitinib . -
pubmed
Thus the effectiveness of
gefitinib requires
growth factor receptor - stimulated
PI3K /
Akt and
MAPK signalling to be intact and functional . -
pubmed
The loss of the
PTEN activity leads to uncoupling of this signalling pathway , determining a partial
gefitinib resistance . -
pubmed
Moreover ,
gefitinib sensitivity may be maintained in these cells through its inhibitory potential in
MAPK /
ERK pathway activity , modulating proliferative
EGFR - triggered events . -
pubmed
Moreover ,
E-64 , an inhibitor of
CTSK , could prevent adipocyte differentiation in a dose - dependent manner , which was characterized by the absence of
triglyceride accumulation and
glycerol contents . -
pubmed
CONCLUSIONS :
CTSK , a
cysteine protease involved in extracellular matrix remodeling , could be one of the determinants of adipocyte differentiation . -
pubmed
Ascorbate itself is oxidized to
dehydroascorbate during the process of antioxidation , and
dehydroascorbate reductase (
DHAR ,
EC 1.8.5.1 ) re - reduces the
oxidized ascorbate . -
pubmed
Salt tolerance was remarkably improved despite slight increases in
DHAR activity and total
ascorbate . -
pubmed
By catabolizing
tryptophan to
N - formyl - kynurenine ,
IDO starves T cells from this important
amino acid rendering them incapable of mounting appropriate immune responses . -
pubmed
IDO - induced
tryptophan depletion from the
tumor microenvironment could be the result of either elevated levels of the enzyme or augmented
tryptophan consumption by both
tumor cells and antigen presenting cells of the host . -
pubmed
Selenium inhibits high
glucose- and high
insulin - induced
adhesion molecule expression in vascular endothelial cells . -
pubmed
Selenium (
Se ) , a biologically essential trace element , can inhibit
cytokine ( e.g. -
pubmed
These experiments tested whether
Se can also alter high
glucose- and high
insulin - induced expression of
adhesion molecules . -
pubmed
RESULTS :
Se ( 100 nmol / L ) significantly inhibited
glucose ( 25 mmol / L)-induced expression of
vascular cell adhesion molecule-1 (
VCAM-1 ) ,
intercellular adhesion molecule-1 (
ICAM-1 ) , and
E - selectin . -
pubmed
Moreover ,
Se significantly inhibited
insulin ( 100 nmol / L)-induced
VCAM-1 and
ICAM-1 expression , whereas high
insulin had no inducing effect on
E - selectin . -
pubmed
Se also inhibited high
glucose- and high
insulin - induced activation of
p38 mitogen - activated protein kinase (
p38 ) , which indicated that the preventive effects of
Se on
adhesion molecules may be associated with
p38 . -
pubmed
The important role of
p38 in
Se effects was further confirmed using
p38 inhibitor
SB203580 . -
pubmed
CONCLUSIONS : These results suggest that
Se can inhibit high
glucose- and high
insulin - induced expression of
adhesion molecules . -
pubmed
We also show that premature delivery is controlled by treatment with the selective
endothelin receptor A antagonist
BQ-123 in a dose - dependent manner . -
pubmed
Gender - associated biological characteristics , likely independent of circulating gonadal
steroids , thus seem to influence
HPA - axis regulation in
depression . -
pubmed
Infusion media composition is
phosphate - buffered saline containing anticoagulants and
human serum albumin . -
pubmed
The time course of hepatocyte viability , funtionality ( drug - metabolizing enzymes , ureogenic capability ,
ATP ,
glycogen , and
GSH levels ) , apoptosis (
caspase-3 activation ) , and attachment and monolayer formation were analyzed . -
pubmed
The optimal preservation of cell viability , attaching capacity , and functionality , particularly
GSH and glycogen levels , as well as drug - metabolizing
cytochrome P450 enzymes , was found in infusion media supplemented with 2 mM
N - acetyl - cystein and 15 mM
glucose . -
pubmed
The results presented here represent the first report of a
kaempferol glycoside as a putative
efflux pump inhibitor in bacteria . -
pubmed
However , the
dolicholpyrophosphate - binding antibiotic
bacitracin failed to inhibit the
acid PPase . -
pubmed
The purified
enzyme showed similar K(m ) values for
PPi ,
ATP , and
ADP to the membrane - bound enzyme . -
pubmed
Serum
vitamin D ,
calcium ,
phosphate , and
parathyroid hormone (
PTH ) concentrations were measured . -
pubmed
However , we observed a significant inverse correlation between
vitamin D concentrations and
PTH . -
pubmed
Lower
vitamin D levels are associated with a higher
PTH concentration . -
pubmed
Down - regulation of
Nrf2 enhanced the
cytotoxicity of
Oxaliplatin ,
Doxorubicin and
Etopside . -
pubmed
Down - regulation of
Nrf2 enhanced the
cytotoxicity of
Oxaliplatin ,
Doxorubicin and
Etopside . -
pubmed
The deduced
amino acid sequence of
SoISG15 shares 60 - 67 % overall identities with the
ISG15 of several fish species . -
pubmed
In this study , we investigated the effects of
zeaxanthin on the migration of skin fibroblasts induced by
PDGF - BB and
melanoma cells . -
pubmed
We demonstrated that
zeaxanthin inhibited
PDGF - BB - induced skin fibroblast migration on
collagen and
gelatin by a modified Boyden chamber system . -
pubmed
However , these effects did not result from direct interaction of
zeaxanthin with
PDGF - BB . -
pubmed
Thus , our results provide the first evidence showing that
zeaxanthin is an effective inhibitor of migration of stromal fibroblasts induced by
PDGF - BB and
melanoma cells and this effect may further support its antitumor potential . -
pubmed
Strong catabolic repression on
protease and
alpha - amylase production was observed with
glucose . -
pubmed
Exercise training reduced epididymal fat , fasting serum
insulin and plasma
glucose disappearance . -
pubmed
We describe the development of the Patient Satisfaction with Treatment Benefit ( PSTB ) questionnaire , and examine the
face , content and criterion validity of this tool in a study of
darifenacin treatment in
OAB patients who expressed dissatisfaction with prior antimuscarinic therapy . -
pubmed
After the
LH peak , the intrafollicular concentration of
17beta - estradiol decreased significantly ( 48.3 + /- 4.4 ng / ml ; p & lt ; 0.001 ) , whereas that of
progesterone increased ( 11690.2 + /- 693.6 ng / ml ; p & lt ; 0.001 ) . -
pubmed
Invariant natural killer T ( iNKT ) cells produce large amounts of
cytokines such as
IL-4 and
IFNgamma upon stimulation with a ligand ,
alpha - galactosylceramide , and regulate various immune responses . -
pubmed
Vanilloid derivatives as
tyrosinase inhibitors driven by virtual screening - based QSAR models . -
pubmed
A number of
vanilloids have been tested as
tyrosinase inhibitors using Ligand - Based Virtual Screening ( LBVS ) driven by QSAR ( Quantitative Structure - Activity Relationship ) models as the multi - agent classification system . -
pubmed
Among them , two chemicals ,
isovanillin ( K(M ) ( app ) = 1.08 mM ) near to
kojic acid ( reference drug ) in one cluster and
isovanillyl alcohol ( K(M ) ( app ) = 0.88 mM ) at the same distance as
hydroquinone ( reference drug ) in another cluster showed inhibitory activity against
tyrosinase . -
pubmed
Glutamate cysteine ligase (
GCL ) catalyzes the rate - limiting step in the de novo synthesis of
glutathione (
GSH ) . -
pubmed
Although several studies suggest that this variation influences
GSH content , its implication for
GCLC expression remains unknown . -
pubmed
These results provide evidence that interaction of the two variations can efficiently impair
GCLC expression and thus suggest its involvement in the pathogenesis of diseases related to
GSH metabolism . -
pubmed
In vitro and in vivo evaluation of
64Cu - radiolabeled KCCYSL peptides for targeting
epidermal growth factor receptor-2 in
breast carcinomas . -
pubmed
In this report , we tested the ability of a bacteriophage selected peptide KCCYSL , radiolabeled with
64Cu , to image
EGFR-2 expressing
breast tumors in vivo by positron emission tomography ( PET ) . -
pubmed
PET /
CT studies revealed successful
tumor uptake of
64Cu - peptides at 2 hours postinjection . -
pubmed
The optimization of bifunctional chelators improves the pharmacokinetic properties of the
64Cu - labeled GSG - KCCYSL peptide , which enables the selection of a suitable peptide homolog as a PET imaging agent for
EGFR-2 expressing
breast carcinomas . -
pubmed
They were i ) Gram - negative , ii )
catalase positive , iii ) produced acid from
glucose and
maltose , iv ) tolerated 5 to 15 %
NaCI v ) except MBRI 9 , showed pH tolerance in the range of 5.0 to 9.0 with optimum pH 7.0 to 8.0 v ) grew well at 30 degrees C and were able to grow in the range of 15 to 45 degrees C.
EPA , an essential
omega-3 fatty acid , was produced by these isolates in the range of 12 to 60 % at 30 degrees C. MBRI 12 was found to be a potential source as it produced 60 %
EPA . -
pubmed
Promising therapeutic effects have been reported in combination with DNA damaging agents ,
taxanes , targeted agents ,
death receptor agonists and hormonal therapies . -
pubmed
The shells are made of
phospholipids , surfactant , denatured
human serum albumin , or synthetic polymer . -
pubmed
Extracellular matrix ( ECM ) adhesion molecules consist of a complex network of
fibronectins ,
collagens , chondroitins , laminins , glycoproteins ,
heparin sulfate ,
tenascins , and proteoglycans that surround connective tissue cells , and they are mainly secreted by fibroblasts , chondroblasts , and osteoblasts ( 7 ) . -
pubmed
In all participants , measurement of height , weight , waist circumference , hip circumference , blood pressure systolic (
SBP ) and blood pressure diastolic (
DBP ) , resting heart rate (
RHR ) , blood
glucose and lipids was carried out using standard methods . -
pubmed
Low Density Lipoprotein -
cholesterol (
LDL - c ) concentrations were calculated using the Friedwald formula . -
pubmed
Triglycerides (
TG ) were significantly higher in urban than rural men ( 1.23 + - 0.39 mmol / L vs 1.17 + - 0.64 mmol / L , p = 0.017 ) . -
pubmed
High Density Lipoprotein -
cholesterol (
HDL - c ) levels were higher in rural compared to urban men ( 2.60 + - 0.10 35mmol / L vs 1.97 + - 1.14 mmol / L , p<0.001 respectively ) . -
pubmed
However , total
Cholesterol (
TC ) and
LDL - c were significantly higher in urban than in rural men ( p<0.001 and p = 0.005 ) and women ( p<0.001 respectively . -
pubmed
BACKGROUND AND PURPOSE :
Ginsenoside - Rd is a
receptor - operated calcium channel antagonist and has shown promise as a neuroprotectant in our phase II study . -
pubmed
Synthesis of
oxo - dihydrospiroindazole - based
acetyl - CoA carboxylase (
ACC ) inhibitors is reported . -
pubmed
Acylation of the resultant
spirocyclic amines provided potent
ACC inhibitors . -
pubmed
In the present study we attempted to elucidate the mechanisms of
glomerular injury induced by
FK506 and the renoprotective effects of the
angiotensin II receptor blocker
telmisartan . -
pubmed
RESULTS :
FK506 induced ROS production via activation of
NAD ( P ) H oxidase in the glomeruli . -
pubmed
FK506 directly promoted
NAD ( P ) H oxidase activity and accelerated production of ROS in hGECs . -
pubmed
Conversely , cotreatment with
telmisartan inhibited both
NAD ( P ) H oxidase activity and production of ROS . -
pubmed
CONCLUSION : These findings suggest that
glomerular injury resulting from
FK506 is caused by oxidative stress mediated by activation of
NAD ( P ) H oxidase and that
telmisartan exerts a renoprotective effect via antioxidative activity . -
pubmed
Inhibition and induction of
glutathione S - transferases by
flavonoids : possible pharmacological and toxicological consequences . -
pubmed
Many studies reviewed herein demonstrated the potency of some
flavonoids to modulate the activity and/or expression of
glutathione S - transferases (
GSTs ) . -
pubmed
The effect of
flavonoids on
GSTs strongly depends on
flavonoid structure , concentration , period of administration , as well as on
GST isoform and origin . -
pubmed
,
acetaminophen ,
simvastatin ,
cyclophosphamide ,
cisplatine ,
polycyclic aromatic hydrocarbons ,
chlorpyrifos ,
acrylamide , and
isocyanates ) , which are
GST substrates , could have significant pharmacological and toxicological consequences . -
pubmed
Although reasonable consumptions of a
flavonoids - rich diet ( that may lead to
GST induction ) are mostly beneficial , the uncontrolled intake of high concentrations of certain
flavonoids ( e.g. -
pubmed
The
serum C3 levels of groups sensitized to
TCE for 24 and 73h were 99.75 + - 1.45 and 93.28 + - 3.61g / ml , respectively , which were significantly lower than that ( 112.30 + - 9.10 g / ml ) of solvent control group ( P & lt ; 0.05 ) . -
pubmed
Also The
serum C4 levels of groups sensitized to
TCE for 24 and 73 h were 34.63 + - 2.53 and 33.82 + - 2.76g / ml , respectively , which were significantly lower than that ( 43.87 + - 3.65 g / ml ) of solvent control group ( P & lt ; 0.05 ) . -
pubmed
The serum
IgA and
IgM levels of groups sensitized to
TCE and unsensitized to
TCE for 24 and 72 h were significantly lower than those of solvent group ( P & lt ; 0.05 ) . -
pubmed
as compared with unsensitized groups , the serum
IgA levels of the groups sensitized to
TCE for 24 and 72 h significantly decreased ( P & lt ; 0.05 ) . -
pubmed
Flow cytometry showed that
MIL induced a
G2 / M arrest and apoptosis in a dose - dependent manner . -
pubmed
Western blot demonstrated that
MIL - induced
G2 / M arrest was correlated with the inhibition of
cyclin - dependent kinase 1 activity , including a remarkable decrease in
cell division cycle ( cdc ) 2 synthesis , the accumulation of phosphorylated -
Thr14 and decrease of phosphorylation at
Thr161 of
cdc2 . -
pubmed
MIL also activated
caspase 9 and
caspase 3 , and significantly increased the ratio of
Bax /
Bcl-2 and stimulated the release of
cytochrome c into cytosol , suggesting
MIL induced apoptosis via mitochondrial apoptotic pathway . -
pubmed
Neu2000 (
NEU ;
2 - hydroxy-5 -( 2,3,5,6 - tetrafluoro-4 - trifluoromethyl - benzylamino )- benzoic acid ) , a recently developed derivative of
acetylsalicylic acid and
sulfasalazine , potently protects against neuronal cell
death following
ischemic brain injury by antagonizing
NMDA receptor - mediated
neuronal toxicity and oxidative stress . -
pubmed
These results suggest that
NEU attenuates
hypoxia - induced impairment of neuronal activity more potently than the antioxidant ,
trolox , and the
NMDA receptor antagonists ,
ifenprodil and
APV . -
pubmed
Fifteen
N - terminal amino acids were identified by Edman degradation , and 77 % of the
rCPI primary structure was confirmed by mass fingerprint . -
pubmed
When the purified
CD13(high)
CD133(+ ) cells were cultured at a low density with feeder cells in the presence of suitable growth factors and signaling inhibitors (
ALK inhibitor
A-83 - 01 and
ROCK inhibitor
Y-27632 ) , individual cells gave rise to relatively large colonies . -
pubmed
alpha - Synuclein oligomers induced by
docosahexaenoic acid affect membrane integrity . -
pubmed
Previously , we demonstrated that
alpha - synuclein forms oligomeric species in the presence of
docosahexaenoic acid and that these species are toxic to cells . -
pubmed
Chromogranin A (
CgA (
CHGA ) ) is the major soluble protein co - stored and co - released with
catecholamines and can function as a pro - hormone by giving rise to several bioactive peptides . -
pubmed
The search strategies utilized keywords such as
chromogranin A , vasostatins 1 and 2 ,
chromofungin ,
chromacin , pancreastatin ,
catestatin , WE14 ,
chromostatin , GE25 ,
parastatin , and serpinin and was supplemented by the screening of references from included papers and review articles . -
pubmed
The analysis of the retrospective literature suggested that
CgA and its several bioactive fragments exert a broad spectrum of regulatory activities by influencing the endocrine , the cardiovascular , and the immune systems and by affecting the
glucose or
calcium homeostasis . -
pubmed
Proline ,
glutamic acid and
leucine - rich protein-1 is essential for optimal
p53 - mediated DNA damage response . -
pubmed
PELP1 - depleted
p53 ( wild - type )
breast cancer cells were less sensitive to various genotoxic agents including
etoposide ,
camptothecin or gamma - radiation . -
pubmed
QCASP3.2 did not exhibit any toxicity and appeared to accurately reflect the induction and inhibition of
caspase activity by
H2O2 and
PACAP , respectively , both in cell lysates and in cultured neurons . -
pubmed
L1 and Pn1
tumor cases displayed a significantly ( p & lt ; 0.05 ) decreased
M1 and increased M2 polarization in the
sinus of the lymph nodes . -
pubmed
G3 cases presented a significantly ( p & lt ; 0.05 ) increased M2 polarization in the
sinus compared to G2 cases . -
pubmed
Photo - induced dynamics of the
heme centers in
cytochrome bc1 . -
pubmed
The c1 - heme
iron - ligand recombination time is 5.4 ps , in agreement with previous reports on homologous
cytochromes . -
pubmed
The different
heme - responses illustrate the flexibility of the
c1 - heme ligation in contrast to the more rigid b -
heme binding , as well as the higher electronic reactivity of the b - hemes within the
bc1 complex . -
pubmed
Here we demonstrated that
PINK1 - 53 is stabilized in the presence of enhanced
Lys-63 - linked ubiquitination and identified
TRAF6 - related
NF - kappaB activation as a novel pathway involved in this . -
pubmed
Twenty - five simulated roots from photoelastic resin were made and divided into 5 groups : CPC , cast
post - and - core ; SP , screw
post ; CF ,
carbon fiber
post ; GF , glass fiber
post ; and CGF , customized glass fiber
post . -
pubmed
After cementing CPC and SP
posts with
zinc phosphate cement , and CF , GF and CGF
posts with resin cement , resin cores were made for groups 2 - 5 . -
pubmed
The antisense BvCMO plants showed the decreased activity of GB synthesis from
choline compared to wild - type ( WT ) plants which is well related to the suppressed level of
BvCMO protein . -
pubmed
[
Collagen and
Taxifolin - based Material : Production and Properties ] . -
pubmed
The possibility of producing the material based on
collagen and biologically active
polyphenol taxifolin was explored , and the properties of the material were studied . -
pubmed
The data on the dynamics of the release of
polyphenol chemically linked to
collagen are represented , and the metal - reducing activity of
polyphenol released from the gel is determined . -
pubmed
It was shown that
polyphenol released from the gel material exerts antioxidant and metal - reducing properties , suggesting that unoxidized
polyphenol linked to
collagen . -
pubmed
We studied how several sub - types of vegetarian diets affect metabolic traits , including waist circumference ,
BMI ,
systolic blood pressure (
SBP ) ,
diastolic blood pressure , fasting blood
glucose , total
cholesterol (
TC ) ,
HDL ,
LDL ,
TAG and TC :
HDL ratio , through both cross - sectional and longitudinal study designs . -
pubmed
In the longitudinal follow - up , each additional year of vegan diet lowered the risk of
obesity by 7 % ( 95 % CI 0 88 , 0 99 ) , whereas each additional year of lacto - vegetarian diet lowered the risk of elevated
SBP by 8 % ( 95 % CI 0 85 , 0 99 ) and elevated
glucose by 7 % ( 95 % CI 0 87 , 0 99 ) , and each additional year of
ovo - lacto - vegetarian diet increased abnormal
HDL by 7 % ( 95 % CI 1 03 , 1 12 ) , compared with non - vegetarians . -
pubmed
In the cross - sectional comparisons , all sub - types of vegetarians had lower likelihoods of abnormalities compared with non - vegetarians on all metabolic traits ( P<0 001 for all comparisons ) , except for
HDL and
TAG . -
pubmed
With proper management of
TAG and
HDL , along with caution about the intake of refined
carbohydrates and
fructose , a plant - based diet may benefit all aspects of the metabolic profile . -
pubmed
A very low
esterase activity and high water activity were observed when perlite , vermiculite and
polyurethane were used as supports . -
pubmed
Moreover , moisture retention was improved by mixing
polyurethane and glass fiber , resulting in maximal biomass and
esterase production . -
pubmed
The application of
hydrogen /
deuterium exchange mass spectrometry (
HDX -
MS ) to localize ligand binding sites in
carbohydrate - binding proteins is described . -
pubmed
Proteins from three bacterial toxins , the B subunit homopentamers of
Cholera toxin and
Shiga toxin type 1 and a fragment of
Clostridium difficile toxin A , and their interactions with native
carbohydrate receptors , GM1
pentasaccharides (
beta - Gal -( 1 3 )- beta - GalNAc -( 1 4 )[ alpha - Neu5Ac -( 2 3 )]- beta - Gal -( 1 4 )- Glc ) , Pk
trisaccharide (
alpha - Gal -( 1 4 )- beta - Gal -( 1 4 )- Glc ) and CD - grease (
alpha - Gal -( 1 3 )- beta - Gal -( 1 4 )- beta - GlcNAcO ( CH2 ) 8CO2CH3 ) , respectively , served as model systems for this study . -
pubmed
Overall , the results of this study suggest that
HDX -
MS can serve as a useful tool for localizing the ligand binding sites in
carbohydrate - binding proteins . -
pubmed
We describe the method to prepare neoglycoproteins from the conjugation of
bovine serum albumin and
pyridylaminated glycans . -
pubmed
These
pyridylaminated glycans can then be conjugated to
bovine serum albumin via esterification with
N - ( m - maleimidobenzoyloxy ) succinimide to form a neoglycoprotein , e.g. -
pubmed
As a demonstration , we prepared High -
mannose bovine serum albumin , which was immobilized on an activated glass slide . -
pubmed
Evaluation of the
gingival inflammation in pregnancy and postpartum via
25 - hydroxy - vitamin D3 ,
prostaglandin E2 and
TNF - alpha levels in saliva . -
pubmed
The aim of the present study was to evaluate saliva levels of
25 - hydroxy - vitamin D3 (
25 ( OH ) D3 ) ,
prostaglandin E2 (
PGE2 ) and
TNF - alpha (
TNF - alpha ) in pregnancy , postpartum and non - pregnant controls . -
pubmed
25 ( OH ) D3 ,
PGE2 and
TNF - alpha levels in the saliva samples were determined by enzyme - linked immunoassays . -
pubmed
RESULTS : Saliva
TNF - alpha and
PGE2 levels were significantly lower and
25 ( OH ) D3 levels were significantly higher in the pregnant group than postpartum group ( p<0.0001 ) . -
pubmed
Saliva
TNF - alpha and
25 ( OH ) D3 levels were significantly higher and
PGE2 levels were significantly lower in the control group than postpartum group ( p<0.0001 ) . -
pubmed
Chloramphenicol resistance allows positive selection of clones after the first event , whereas a colorimetric assay based on the
xylE gene provides a simple way to screen clones in which the second recombination event occurs . -
pubmed
Abeta can induce astrocyte activation which give rise to produce reactive
oxygen species ( ROS ) and cytotoxic
cytokines and
chemokines , and subsequently induce neuronal
death . -
pubmed
Four different
transition metal complexes containing
dipyridyl triazole ligands , namely
[ Cu ( abpt ) 2Cl2 ] 2H2O ( 1 ) ,
[ Cu ( abpt ) 2 ( ClO4 ) 2 ] ( 2 ) ,
[ Co2 ( abpt ) 2 ( H2O ) 2Cl2 ] Cl2 4H2O ( 3 ) and
[ Co2 ( Hbpt ) 2 ( CH3OH ) 2 ( NO3 ) 2 ] ( 4 ) have been designed , synthesized and further structurally characterized by X - ray crystallography , ESI -
MS , elemental analysis , IR and Raman spectroscopy . -
pubmed
However ,
RA subjects using current anti -
TNF therapy ( n = 13 , disease activity score 1.98[1.8 - 2.2 ] ) have an almost 1.2 - fold higher
( 18 ) F - FDG uptake in the arterial wall compared to those using DMARDs ( but with previous anti -
TNF therapy ) ( n = 10 , disease activity score 2.24[1.3 - 2.5 ] ) , which seemed to be predominantly explained by longer duration of their
rheumatic disease in a multivariate linear regression analysis . -
pubmed
Carbon capture and storage (
CCS ) provides a solution toward decarbonization of the global economy . -
pubmed
Increase in dietary
aflatoxin level resulted in the decreased villi height , villi height - to - crypt depth ratio (
VH :
CD ) , villi surface area and apparent villi absorptive area , while it increased crypt depth , goblet cell count and lymphoid follicular diameter . -
pubmed
BACKGROUND : Expression of the
androgen receptor splice variant 7 (
AR - V7 ) is associated with poor response to second - line endocrine therapy in
castration - resistant prostate cancer (
CRPC ) . -
pubmed
Zepatier is a fixed - dose combination product coformulating
MK-5172 [
grazoprevir (
GZR ) , 100 mg QD ] and
MK-8742 [
elbasvir or (
EBR ) 50 mg QD ] : it combines highly potent inhibitors of the
HCV NS3 / 4A protease and
NS5A replication complex , respectively . -
pubmed
Prototypical SOCs are formed of
Orai1 proteins and are activated by the endo / sarcoplasmic reticulum
Ca2 + sensor
stromal interaction molecule 1 (
STIM1 ) . -
pubmed
In
Orai1-/- VSMCs , store depletion induced
PLCbeta1 activity measured with the fluorescent
phosphatidylinositol 4,5 - bisphosphate /
inositol 1,4,5 - trisphosphate biosensor GFP -
PLCdelta1 - PH , which was prevented by knockdown of
STIM1 . -
pubmed
Study outcomes included the incidence of
bladder perforation and
obturator jerk , decrease in
hemoglobin and
sodium levels , rates of re - coagulation and blood transfusion , TUR
syndrome , and operation time . -
pubmed
However , with a decrease in the
hemoglobin and
sodium levels , rates of re - coagulation and blood transfusion were greater in the monopolar arm . -
pubmed
Pyk2 phosphorylation of
VE - PTP downstream of
STIM1 - induced
Ca2 + entry regulates disassembly of adherens junctions . -
pubmed
Here we investigated the role of
stromal interaction molecule 1 (
STIM1 ) activation of store - operated
Ca2 + entry ( SOCE ) in regulating AJ assembly . -
pubmed
Pyk2 - induced
tyrosine phosphorylation of
VE - PTP promoted
Src binding to
VE - PTP ,
Src activation , and subsequent
VE - cadherin phosphorylation and thereby increased the endothelial permeability response . -
pubmed
Thus
Pyk2 activation secondary to
STIM1 - induced
SOCE causes
tyrosine phosphorylation of
VE - PTP , and
VE - PTP , in turn , binds to and activates
Src , thereby phosphorylating
VE - cadherin to increase endothelial permeability through disassembly of AJs . -
pubmed
Our results thus identify a novel signaling mechanism by which
STIM1 - induced
Ca2 + signaling activates
Pyk2 to inhibit the interaction of
VE - PTP and
VE - cadherin and hence increase endothelial permeability . -
pubmed
Vitamin C , high sensitive
C - reactive protein (
hs - CRP ) , soluble
intercellular cell adhesion molecule 1 (
sICAM-1 ) , and soluble
vascular cell adhesion molecule 1 (
sVCAM-1 ) were measured in a cohort of 598 RTR . -
pubmed
A novel
oxynitride compound ,
Pr4 - xCaxSi12O3 + xN18 - x , synthesized using a solid - state route has been characterized as a monoclinic structure in the
C2 space group using Rietveld refinement on synchrotron powder X - ray diffraction data . -
pubmed
TEM showed that the SWNTs with
pyridine functionality more rapidly produced a more even distribution of
copper seeds with a narrower size distribution ( 3 - 12 nm for
py - US - SWNTs ) than those SWNTs with
oxygen functional groups ( ca . -
pubmed
In this study , a pair of water - soluble chiral
3,4 - ethylenedioxythiophene (
EDOT ) derivatives ,
( R )- 2 '- hydroxymethyl-3,4 - ethylenedioxythiophene (
( R )- EDTM ) and
( S )- 2 '- hydroxymethyl-3,4 - ethylenedioxythiophene (
( S )- EDTM ) , were synthesized and electrodeposited on the surface of a
glassy carbon electrode (
GCE ) via current - time ( I - t ) polymerization in an aqueous
LiClO4 electrolyte . -
pubmed
The results indicated that the
( R )- PEDTM /
GCE sensor showed a higher peak current response toward the levo or ( S ) forms of the tested enantiomers , while the opposite phenomenon occurred for
( S )- PEDTM /
GCE . -
pubmed
AAs are vitally important for proper neurodevelopment , as they comprise the most abundant neurotransmitters in the brain and act as neurotransmitter precursors ,
nitric oxide donors , antioxidants , and
neurotrophic factors , which induce synaptogenesis , neuronal proliferation , and migration . -
pubmed
We conclude that
OR and site 5
histidine substitutions have divergent phenotypic impacts and that cis interactions between the OR region and the site 5 region modulate pathogenic outcomes by affecting the
PrP globular domain . -
pubmed
2 - aminopurine suppresses the
TGF - beta1 - induced epithelial - mesenchymal transition and attenuates
bleomycin - induced
pulmonary fibrosis . -
pubmed
This compound significantly attenuated
bleomycin (
BLM)-induced
pulmonary inflammation , the
EMT , and
fibrosis . -
pubmed
Collectively , we determined that 2 - AP could inhibit
metastasis in vitro by suppressing the
TGF - beta1 - induced
EMT and could attenuate
BLM - induced
pulmonary fibrosis in vivo . -
pubmed
In this study , we demonstrate that
meta - tetra ( hydroxyphenyl ) chlorin (
mTHPC)-based photodynamic therapy combined with
bevacizumab (
Avastin ) , an anti -
VEGF neutralizing monoclonal antibody that blocks the binding of
VEGF to its receptor , can enhance the effectiveness of each treatment modality . -
pubmed
Enzyme - linked immunosorbent assay ( ELISA ) and immunohistochemistry ( IHC ) were performed to assess
VEGF concentrations and microvessel density in the various treatment groups , and confocal imaging and high performance liquid chromatography ( HPLC ) analyses were used to measure the distribution and concentration of
mTHPC in
tumors . -
pubmed
Immunostaining and ELISA analyses revealed a lower expression of
VEGF in
tumors treated with combination therapy of PDT followed by
bevacizumab . -
pubmed
UDG catalyzes the removal of
uracil base from the enclosed dumbbell - shape DNA substrate to give an apyrimidinic site , at which the substrate oligonucleotide is cleaved by
endonuclease IV . -
pubmed
Part
II :
Phenolic compounds profile . -
pubmed
Peptide polymerization relies on
tyrosinase oxidation of
tyrosine residues to
Dopaquinones , which rapidly form
cysteinyldopa - moieties with free
thiols from
cysteine residues , thereby linking unimers and generating adhesive polymers . -
pubmed
Chromatographic separation coupled with high - resolution mass spectrometry revealed
beta - galactosidase degradation of several beta - linkage - containing BMOs and production of novel
oligosaccharides that ranged in size from 5 to 11
monosaccharide units containing several
galactose repeating units and
N - acetylhexosamine at their reducing ends . -
pubmed
Fourier transfer infrared ( FTIR ) , Nuclear Magnetic Resonance ( NMR ) and thermogravimetric (
TG ) analysis revealed that
DOPO and
DPSD are linked to EGTO by a covalent bond . -
pubmed
Olopatadine , a topical
H1 antagonist used in the treatment of
ocular allergies , may interfere with the humoral regulation of the iris arteriolar tone . -
pubmed
Thermal and chemical unfolding of
lysozyme in the presence of
guanidineHCl denaturant is a model system to compare the conventional 2 - state model of protein unfolding with the multi - state Zimm - Bragg theory . -
pubmed
Lysozyme unfolding is associated with a large endothermic enthalpy that decreases significantly upon addition of
guanidineHCl . -
pubmed
The Zimm - Bragg theory provides the protein cooperativity parameter , the average length of independently folding
protein domains , and the Gibbs free energy of unfolding of individual
amino acid residues . -
pubmed
GuanidineHCl binding to
lysozyme is exothermic and counteracts the endothermic unfolding enthalpy . -
pubmed
The number of bound denaturant molecules is determined from the decrease in enthalpy and is extrapolated to the
guanidineHCl - to -
amino acid stoichiometry at complete
lysozyme unfolding . -
pubmed
Two alkaline - earth coordination compounds ,
[ Ba ( C8H4N4O2 )( H2O ) 4 ] n , ( I ) , and
[ Sr ( C8H4N4O2 )( H2O ) 3 ] n , (
II ) , from the one -
pot hydrolysis transformation of
benzoyl chloride and the in situ self - assembled [ 2 + 3 ] cyclo - addition of
nitrile are presented . -
pubmed
Protective effect of
proton pump inhibitors and
potassium competitive acid blockers against
post - gastric endoscopic submucosal dissection
bleeding : a single - center , propensity score - matched analysis . -
pubmed
OBJECTIVES : Both
potassium - competitive acid blockers ( P - CABs ) and
proton pump inhibitors ( PPIs ) are known to be protective against
bleeding after gastric endoscopic dissection (
ESD ) for early
gastric cancers . -
pubmed
The aim was to compare the effect of PPI and
P - CAB treatment against
bleeding after gastric
ESD . -
pubmed
Reconstitution of a
Ca2 + - transporting ATPase system from
triton X-100 - solubilized sarcoplasmic reticulum . -
pubmed
L - DOPA ,
dopamine , and
tyrosine were found to be good substrates for this
enzyme . -
pubmed
Vmax =
14.1 , 8.1 , and 6.1 mumoles
O2 / min / mg
protein for each substrate , respectively . -
pubmed
The relationship between the
pulmonary toxicity of
3 - methylindole (
3MI ,
skatole ) and the
mixed - function oxidase (
MFO ) system was investigated . -
pubmed
Results indicate that the
MFO system is one of the pathways involved in the metabolism of
3MI and that
pulmonary toxicosis results from metabolism of
3MI by this
enzyme system . -
pubmed
The inability of a peptide to substitute for the requisite
amino acid was not due to the absence of cellular
peptidases or to toxicity of the nonutilized peptides . -
pubmed
In the bound state , the
enzyme exhibits a greater affinity for
glutamate , but a lower affinity for
oxalacetate . -
pubmed
A similar effect was elicited by a
D - stereoisomer of propranolol with no
beta - adrenoceptor blocking activity . -
pubmed
In the observations reported here , the
HMB45 - defined
antigen was identified in another
pigmented tissue , the retinal
pigment epithelium (
RPE ) . -
pubmed
Pre - treatment of deparaffinized tissue sections with
neuraminidase completely eliminated
HMB45 immunoreactivity , suggesting that the antigen(s ) recognized is a sialated
glycoconjugate . -
pubmed
A wide series of
restriction enzymes with a range of specificities was used to investigate the interaction with DNA of
Ru ( II )- DMSO complexes with anticancer activity . -
pubmed
While
cis - RuCl2 (
DMSO)4 was almost completely inactive , treatment of pBR 322 DNA with
trans - RuCl2 (
DMSO)4 protected G rich sequences from cutting of specific
restriction endonucleases , indicating a preferential interaction with adjacent
guanines . -
pubmed
The possible involvement of
cyclic AMP in the control of the
PEPCK activity of the primary renal cells was indicated by the stimulatory effects of
8 bromocyclic AMP ,
isobutyl methylxanthine ( a
cyclic AMP phosphodiesterase inhibitor ) , and
forskolin ( an activator of
adenylate cyclase ) . -
pubmed
Phorbol 12 - myristate 13 - acetate (
TPA ) , which activates
protein kinase C , was inhibitory . -
pubmed
Some of the former co - expressed
HLA - DR antigens but not
CD1a antigens . -
pubmed
AT
II ,
elymoclavine and
bromocryptine were applied alone . -
pubmed
Elymoclavine potentiates the effect of
AT II during exploratory behaviour . -
pubmed
The effects of the drugs tested on the exploratory behaviour most probably result from the interaction between
AT II receptors ,
dopamine receptors and through
GABA - ergic neurotransmission , in the respective brain zones responsible for behaviour . -
pubmed
An attempt was made to predict clinical variants of a course of
DTG using
HLA - antigens . -
pubmed
Gel column chromatography revealed that the pituitary ir
FGF was present mainly as high molecular weight forms , with only a small proportion as the basic 146
amino - acid peptide . -
pubmed
In order to avoid cross - reactions with other members of the
keratin multigene family , appropriately taylored
35S - labeled cDNA probes comprising the low and non - homologous 3 ' coding and noncoding domains of the mRNAs were used for in situ hybridization to tissue sections . -
pubmed
It was shown that
IDF45 acted in the
G1 phase of the cell cycle . -
pubmed
Furthermore ,
IDF45 inhibited the early stimulation of RNA synthesis induced by
alpha - globulin . -
pubmed
By comparing
amino acid sequences of Id+ and Id- anti - alpha(1 - ---6)dextrans , the major idiotype was assigned to residues in
VH CDR3 , with a possible contribution from VH CDR2 , a conclusion supported by the
hapten inhibition results . -
pubmed
Initially , both groups had a similar
body mass index , resting heart rate and blood pressures , blood
glucose and
hemoglobin A1 . -
pubmed
Although total
cholesterol was found to be reduced by 13 % for the exercise group ( P less than 0.03 ) and 11 % for the controls ( P less than 0.01 ) , a 15 % decrease ( P less than 0.03 ) in
high - density lipoprotein (
HDL ) was observed for the control group , only . -
pubmed
In this report the
p beta H alpha-5 clone was used to investigate whether patients with two different variants of
Tay - Sachs disease also lack the alpha - chain
message . -
pubmed
In the acute phase there was a tendency to a negative correlation of erythrocyte sedimentation rate and of
C - reactive protein with serum
cholesterol and/or
HDL cholesterol . -
pubmed
Both doses of
5 - hydroxytryptamine elicit a strong release of
GH , the effect being dose - dependent . -
pubmed
In pituitaries perifused with
5 - hydroxytryptamine ( 100 microM during 115 min or 1 , 10 and 100 microM during 15 min ) , a significant release of
GH was also observed . -
pubmed
These results suggested that
5 - hydroxytryptamine may stimulate
GH secretion through a direct pituitary action . -
pubmed
Attempts at histochemical localization of
estrogen receptor with anti -
steroid antibody or some
fluoresceinated estrogens have given unacceptable sensitivities and specificities when compared with biochemical methods or clinical response . -
pubmed
Cysteine specific activity was quantitated , and found to decline rapidly after injection of the labeled
amino acid : less than 0.1 % of the injected label remained as free
cysteine in the hypothalamus 30 min
post injection . -
pubmed
Incorporation of label into
SRIF-14 and
SRIF-28 reached maximum values 8 h
post injection , compared with a labeling maximum at 1 - 2 h for acid - precipitable protein . -
pubmed
Within 2 h after
[ 35S ] cysteine injection , nearly half of the total labeled hypothalamic
SRIF appeared in the medial basal hypothalamus ; 24 - h
post injection this percentage reached approximately 75 % . -
pubmed
Colchicine administration dramatically reduced the appearance of labeled
SRIF in medial basal hypothalamus , but had no apparent effect on the total incorporation of
[ 35S ] cysteine into
SRIF-14 ,
SRIF-28 , or acid - precipitable protein , or on radioimmunoassayable
SRIF levels . -
pubmed
[
ATPase activity and
ATP - dependent accumulation of
Ca2 + in skeletal muscle nuclei . -
pubmed
An addition of
Ca2 + significantly increased the activity of
nuclear ATPase . -
pubmed
The changes in
Ca - ATPase activities and in the
ATP - dependent uptake of
45Ca induced by denervation and electric stimulation were found to be synchronous and unidirectional . -
pubmed
The effects of
triethyltin bromide on red cell and brain
cyclic AMP - dependent protein kinases . -
pubmed
Additions of 25 - 500 microM
triethyltin to red cell ghosts resulted in enhanced phosphorylation of
ghost proteins . -
pubmed
Brief exposure of the partially purified
enzymes to
triethyltin , followed by
DE52 chromatography , resulted in elution profiles for regulatory and catalytic subunits that were similar to the profile resulting after
cyclic AMP activation . -
pubmed
The inhibition was non - competitive with respect to both
histone and
ATP . -
pubmed
When intact red cells were subjected to brief exposure with
triethyltin , enhanced phosphorylation of certain membrane proteins occurred , suggesting that the activation of the
cyclic AMP protein kinases by
triethyltin may be physiologically significant . -
pubmed
Enzymatic activity of the protein was detectable only in assays containing highly purified
gamma - glutamyl phosphate reductase , the second enzyme of the
proline pathway . -
pubmed
Plots of
gamma - glutamyl kinase activity as a function of
glutamate concentration were sigmoidal , with a half - saturation value for
glutamate of 33 mM , whereas plots of
enzyme activity as a function of
ATP concentration displayed typical Michaelis - Menten kinetics with a Km for
ATP of 4 X 10(-4 ) M.
Enzyme activity was insensitive to the
glutamate analog
L - methionine - DL - sulfoximine , but
ADP was a potent competitive inhibitor . -
pubmed
These results indicated that the only major difference was that the
enzyme from the
3,4 - dehydroproline - resistant
strain was 100 - fold less sensitive to feedback inhibition by
proline . -
pubmed
These findings strongly suggest that the combination of 5 - Fu and
HuIFN - alpha or
5 - Fu alone is selectively effective on the neoplastic cells of
HSG cell population but not on the non - neoplastic cells . -
pubmed
The
enzyme was maximally active at pH 7.6 in the presence of 4 mM
calcium . -
pubmed
The
enzyme was labile in aqueous solutions and storage at 4 degrees C and --20 degrees C resulted in considerable loss of activity , unless
glycerol ( 50 % v / v ) was added to the solution . -
pubmed
The behaviour of blood lead (
PbB ) and of some indicators of effect (
erythrocyte protoporphyrin IX (
EP ) ,
delta - aminolaevulinic acid dehydratase activity of erythrocytes (
ALAD ) , and urinary
delta - aminolaevulinic acid (
ALAU ) ) were studied in subjects who had ceased working with inorganic lead for at least one year . -
pubmed
The relation between
EP and
PbU - EDTA shows that , corresponding levels of chelatable lead , the values of the erythrocyte metabolite are identical in the two groups . -
pubmed
Considering the
EP - PbB relation , however , at the same
PbB levels the
protoporphyrin values appear distinctly more altered in the subjects with past exposure . -
pubmed
Similar results were obtained from a study of the relations between
ALAD and
PbU - EDTA and between
ALAD and PbB. -
pubmed
On the basis of the results obtained it is concluded that in subjects with past exposure ,
EP and
ALAD can be used in establishing the persistence and extent of an " active deposit " of lead in the organism , while
PbB is of very limited use . -
pubmed
The present results showed that
ANF enhanced the salivation induced by pharmacological agents which stimulate
phosphatidylinositol hydrolysis . -
pubmed
These effects of
ANF may be probably related to the activation of the non - guanylate cyclase coupled receptor which has been associated with
phosphatidylinositol turnover . -
pubmed
Leukocytic DNA was extracted from the blood of 93 patients after which the region containing two mutation points coding
amino acid residues 112 and 158 was amplified by using the
polymerase chain reaction ( PCR ) . -
pubmed
The
alpha - tocopherol - binding protein did not bind
oleate as assessed by direct radiolabeled
fatty acid binding and fluorescence enhancement assay . -
pubmed
The Western blot , ligand affinity , molecular size , and
amino acid analysis data suggest that the
alpha - tocopherol - binding protein is different from the
cytosolic fatty acid - binding protein and that it may be involved in intracellular transport and metabolism of
alpha - tocopherol . -
pubmed
The selective
tissue kallikrein inhibitor
CH-694 ( but not the selective
plasma kallikrein inhibitor
CH-684 ) caused highly significant falls in airways resistance when it was administered at 10 mg / kg intraperitoneally 15 min before and 90 min after challenge . -
pubmed
BACKGROUND :
Taxol is a new anticancer drug that acts as a
tubulin polymeration enhancer . -
pubmed
Reaction of
7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole with the
( Ca2 + + Mg2 +)- ATPase protein of sarcoplasmic reticulum at low temperature . -
pubmed
Modification of the
( Ca2 + + Mg2 +)- ATPase protein of
rabbit skeletal sarcoplasmic reticulum (
SR ) with
7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole ,
NBD - Cl , at 4 degrees C for 5 min caused a 63 % loss of the
Ca ( 2 +)-dependent
ATPase activity when 1 mol of the
adenine analog was incorporated per 10(5 ) g of protein . -
pubmed
MgATP protected the
ATPase activity at 4 degrees C and 25 degrees C but there was little change in the extent of labeling at 4 degrees C suggesting that changes in the fluidity of the lipid moiety made different sites on the
ATPase protein accessible to the reagent . -
pubmed
At 4 degrees C , addition of
sodium deoxycholate enhanced the inactivation ( 6 %
ATPase activity remained ) but the labeling of the
SR - ATPase protein did not increase significantly . -
pubmed
Incubation with
MgATP prior to solubilization with
deoxycholate resulted in the protection of the
Ca ( 2 +)- ATPase activity and only a small decrease in the labeling occurred . -
pubmed
At 25 degrees C , a similar pattern was found with
deoxycholate but the loss of
ATPase activity was less dramatic and the extent of labeling by
NBD - Cl was greater than that at 4 degrees C.
MgATP induced changes in the conformation of the
ATPase protein protecting essential
cysteine residues while shifting the reaction of
NBD - Cl with the
ATPase protein to non - essential sites in the absence or presence of
deoxycholate . -
pubmed
An analysis of tryptic digests of the
NBD - ATPase protein showed that
MgATP shifted the labeling from the
A2 subfragment to the
A1 subfragment in the absence of
deoxycholate and from the
A1 subfragment to the
A2 subfragment in the presence of
deoxycholate . -
pubmed
The reagent ,
NBD - Cl , can distinguish between different temperature dependent conformational states of the
ATPase protein . -
pubmed
SN2 reaction of the known
cyclopentenyl units 12 and 13 with
2 - haloadenines under basic conditions gave the protected
carbocyclic nucleosides 14b , c and 15b , c , respectively . -
pubmed
No evidence for association of
dopamine D2 receptor variant (
Ser311 /
Cys311 ) with
major psychosis . -
pubmed
We investigated a variant of the
dopamine D2 receptor gene (
Ser311 /
Cys311 substitution ) in Caucasian patients with
schizophrenia ( n = 273 ) ,
delusional disorder ( n = 62 ) ,
bipolar I affective disorder ( n = 63 ) , and controls ( n = 255 ) . -
pubmed
However ,
anti - ANT antibody can inhibit the
ADP /
ATP exchange of heart mitochondria and be organ - specific . -
pubmed
An HPLC method is described for simultaneously obtaining the enantiomeric ratio of three
amino acids (
aspartic acid ,
serine , and
glutamic acid ) from dental
collagen , with a view to using this information for estimating age at
death . -
pubmed
Pancreastatin (
PST ) , a recently discovered regulatory peptide derived from
chromogranin A , has been shown to have a glycogenolytic effect in the hepatocyte that is mediated by increasing intracellular
calcium . -
pubmed
Since cerebral metabolic activity reflects regional brain work for all neurotransmitter systems , imaging metabolism directly with
fluorodeoxyglucose and indirectly with blood flow and
hemoglobin oxygen saturation can provide information about the functional neuroanatomy of a deficit in individual patients and allow patients to be grouped into more homogeneous subgroups for intensive study . -
pubmed
PURPOSE :
Acute anterior uveitis is strongly associated with the
HLA - B27 antigen and triggered by the involvement of some external factors . -
pubmed
White
phosphorus affected several plasma constituents , most notably elevated
aspartate aminotransferase , blood
urea nitrogen ,
lactate dehydrogenase , and
alanine aminotransferase . -
pubmed
The presence of a
methyl group on the
piperazine moiety decreased the pharmacodynamic contribution to the convulsant activity by severalfold , and the ratios of concentrations of the FQs in cerebrospinal fluid (
CSF ) to concentrations of unbound FQs in plasma varied from about 5 to 75 % as a function of lipophilicity . -
pubmed
This effect was evidenced by a reduction of cell viability in the
MTT assay ,
G1 arrest and significant apoptosis upon flow - cytometric analysis , presence of apoptotic bodies , formation of DNA ladders upon gel electrophoresis and inhibition of viral early gene expression by RT - PCR and Western blot . -
pubmed
NSAIDs cause
gastrointestinal damage by 2 independent mechanisms : a topical effect , which is pH and pKa related , and a systemic effect mediated by
cyclooxygenase (
COX ) inhibition with a reduction in
prostaglandin synthesis . -
pubmed
Among the strategies used to decrease the risk of
ulcer development are : ( i ) the use of analgesics other than NSAIDs ; ( ii ) use of the lowest possible dosage of NSAID ; ( iii ) the use of a
COX-2 selective NSAID ; ( iv ) the use of low doses of
corticosteroids instead of NSAIDs ; ( v ) avoidance of concomitant use of NSAIDs and
corticosteroids ; and ( vi ) use of preventive therapy . -
pubmed
In an attempt to reduce the incidence of NSAID - induced
gastrointestinal lesions , the following approaches have been proposed : ( i ) use of the
prostaglandin analogue
misoprostol , which is an antiulcer drug which has been proven to be as effective in the prevention of NSAID - induced
gastric and duodenal ulcers as in the reduction of serious upper
gastrointestinal complications ; ( ii )
histamine H2 receptor antagonists (
H2 antagonists ) , e.g. -
pubmed
ranitidine ,
cimetidine and
famotidine , which are useful in the prevention of NSAID - induced
duodenal ulcers during long term treatment , but not in the prevention of NSAID - induced
gastric ulcers ; ( iii )
proton pump inhibitors , e.g
omeprazole , and
pantoprazole , whose efficacy in preventing NSAID - associated
ulcers has been recently demonstrated ; and ( iv ) barrier agents , e.g. -
pubmed
Incubation of guinea - pig peritoneal macrophages with
cholera toxin (
CT ) or
aluminium hydroxide gel (
Al - gel ) released a significantly higher activity of
lactate dehydrogenase than did commercial natural
CTB (
CTB ) or rCTB . -
pubmed
Intraintestinal or intramuscular administration of
CT ,
CTB or
Al - gel caused severe histopathological reactions . -
pubmed
Interactions of
human fibrinogens with
factor XIII : roles of
calcium and the gamma ' peptide . -
pubmed
Factor XIII binds
fibrinogen gamma ( A )/ gamma ' approximately 20 - fold more tightly than
fibrinogen gamma ( A )/ gamma ( A ) , and the interaction with
fibrinogen gamma ( A )/ gamma ' ( but not
fibrinogen gamma ( A )/ gamma ( A ) ) is accompanied by a significant release of
Ca ( 2 + ) . -
pubmed
Because of its induction during
carbohydrate and complete
starvation ( incubation in tap water ) and
CdCl ( 2 ) and
bile salts stresses , one of these proteins (
Gls24 ) was qualified as a " general stress protein " and was analyzed at the molecular level . -
pubmed
A mutation in the
gls24 gene led to a severe reduction of growth rate and reduction of survival against 0.3 %
bile salts in the 24 - h - starved cells compared to the wild - type
strain . -
pubmed
Three of these sequences map in genes coding for
L - lactate dehydrogenase ,
lipoamide dehydrogenase , and
pyruvate decarboxylase , all involved in
pyruvate metabolism . -
pubmed
The expression of
P - selectin and
fibrinogen binding was not affected by
bezafibrate therapy . -
pubmed
Under voltage
clamp , the initial rate of increase in [
Ca2 +]( i ) in guard cells is sensitive to the extracellular divalent concentration , suggesting a close interaction between the permeant ion and channel gating . -
pubmed
Low
Na superfusion significantly suppressed the relaxation and decreased the
rest potentiation in the myocardial preparations all ages . -
pubmed
The PCR results and the ability of
1 - oleoyl - LPA to mimic these drugs ( also with respect to their susceptibility to antagonists ) suggest that the release - inhibiting receptor may be an
edg - encoded
lysophospholipid receptor . -
pubmed
For each subject , blood samples were measured for complete blood count , reticulocyte count , percentage of hypochromic red cells ( %
HRC ) ,
serum ferritin , serum
iron ,
transferrin saturation (
TS ) ,
serum erythropoietin (
sEpo ) ,
C - reactive protein (
CRP ) , and
sTfR. -
pubmed
Our study showed that
sTfR levels quantitatively reflect the integrated effects of
iron availability ,
iron reserves , and erythropoietic stimulation . -
pubmed
Pro - aqualysin I ( 38kDa ) as inactive protein was secreted into the medium of shake - flask cultures at a concentration of 1g / L. It was isolated from the culture supernatant by an
ammonium sulfate precipitation and one - step anion exchange chromatography in a nearly pure form and was autoproteolytically activiated by heat treatment . -
pubmed
Paper electrophoresis as a quantitative method : measurement of
alpha and beta lipoprotein cholesterol . -
pubmed
Phosphatidylinositol - specific phospholipase C ( PLC ) enzymes catalyze hydrolysis of
phosphatidylinositol 4,5 - bisphosphate (
PIP2 ) generating
diacylglycerol (
DAG ) and
inositol 1,4,5 - triphosphate (
IP ( 3 ) ) . -
pubmed
In the case of
hexahistidine ( 6 - His)-tagged
PLC beta the purification involves two steps , affinity chromatography with
Ni - NTA agarose followed by heparin Sepharose chromatography . -
pubmed
6 - His - tagged
PLC epsilon can be purified in a single step with
nickel nitrilotriacetic acid -
agarose ( Ni - NTA ) affinity chromatography . -
pubmed
Other
bisphosphonates (
pamidronate ,
alendronate , and
incadronate ) did not increase
alkaline phosphatase activity in either cell line . -
pubmed
These data suggest that
clodronate acts directly on mesenchymal cells to enhance osteoblast differentiation , and this effect may be partly expressed through inhibition of
protein Tyr phosphatase and/or
Ser / Thr phosphatase activity . -
pubmed
The title compound crystallizes in space group
P21 / c with two molecules in the asymmetric unit , both of which exhibit disorder in one of their
thiophene rings . -
pubmed
Maximum electrode response was found in
phosphate buffer at pH 8.0 and 35 degrees C. The reproducibility of the
enzyme electrode was also tested by using standard
phenol solutions ( 0.005 microM ) . -
pubmed
Thirty female Small East African goat kids at an average age of 5 months were initially treated with
albendazole orally ( 5 mg kg(-1 ) body mass ) and randomly assigned into one of two groups : group I were fed UMB prepared using a cold process and group
II kids ( controls ) received no block supplementation (
NBS ) . -
pubmed
These results suggest that the accumulation of
amino acid substitutions in the
HA protein during evolution promoted irreversible structural changes and therefore that antigenic changes in the
H3HA protein may not be limited . -
pubmed
None of the
gonadoblastomas showed immunoreactivity with antibodies against
steroid hormone receptors and against
testosterone and
estradiol , respectively . -
pubmed
Removal of both low - molecular - weight components and
histones increased the yield of
8 - oxo - dG 46 - fold and the yield of strand breaks 43 - fold . -
pubmed
Removal of only the
histones thus leads to a two times greater increase in the yield of strand breaks compared to
8 - oxo - dG. -
pubmed
These results suggest that in intact cells ,
8 - oxo - dG is preferentially formed in
histone - free structures of chromatin , indicating a larger role for the indirect effect of radiation in the formation of
8 - oxo - dG than in the formation of strand breaks . -
pubmed
Enzymically hydrolysed DNA was subjected to HPLC -
MS /
MS analysis , which demonstrated that the phiR1 - 37 genome is composed of DNA in which
thymidine ( T ) is & gt;99 % replaced by
deoxyuridine ( dU ) . -
pubmed
N - terminal
amino acid sequences of four major structural proteins did not show any similarity to ( viral ) protein sequences in databases , indicating that close relatives of
phiR1 - 37 have not yet been characterized . -
pubmed
GSTM1 ( - ) smokers had a significantly higher level of plasma DNA adducts and leukocytes level of
8 - OHdG than their
GSTM1 ( + ) counterparts ( P & lt ; 0.01 ) . -
pubmed
Only the
GSTM1 ( - ) subgroup was the one that , under
FPP treatment , increased lymphocyte
8 - OHdG ( P & lt ; 0.01 ) . -
pubmed
In spite of the nonsulfidic conditions and abundant reactive
iron ( III ) commonly found in mobile tropical deltaic muds , genes encoding dissimilatory
sulfite reductase (
dsr ) were successfully amplified from the upper approximately 1 m of coastal deposits sampled along French Guiana and in the Gulf of Papua . -
pubmed
The spatial and temporal distribution of
dsr sequences strongly supports the contention that the
sulfate - reducing prokaryote communities in mobile mud environments are cosmopolitan and stable over a period of years . -
pubmed
Recently , association of an
interleukin-1A promoter polymorphism ( -889 ,
thymine /
thymine ( T / T ) ) with
Alzheimer ' s disease was reported , suggesting that this
cytokine may play an important role in disease development . -
pubmed
Indirect staining of
antibodies using
fluorescein isothiocyanate indicated
CRA were concentrated mainly around epidermal and mesophyll cells in compatible host ( T-78 ) . -
pubmed
This study aimed to evaluate a small - molecule
PAI-1 inhibitor (
PAI-039 ;
tiplaxtinin ) in a rodent
stenosis model of
venous thrombosis in a two - phase experiment . -
pubmed
PAI-039 treatment was also associated with significantly increased return of inferior vena cava blood flow four days
post -
thrombosis versus controls ( p & lt ; 0.05 ) . -
pubmed
RV09 , a novel
resveratrol analogue , inhibits
NO and
TNF - alpha production by LPS - activated microglia . -
pubmed
Moreover ,
RV09 attenuated the expression of mRNA and protein of
tumor necrosis factor - alpha (
TNF - alpha ) in a concentration - dependent manner . -
pubmed
In the summary , these data suggest that , by blocking
IkappaBalpha phosphorylation and degradation ,
RV09 acts to suppress the LPS - induced
NO and
TNF - alpha production in microglia , and this effect was mediated , at least in part , by inhibiting the generation of ROS . -
pubmed
Insulin secretion was estimated by acute
insulin response (
AIR ) during an intravenous
glucose - tolerance test ( IVGTT ) , and the disposition index was calculated as
AIR x M(FFM ) . -
pubmed
M(FFM ) was higher in
MHO who exhibited lower waist circumference , blood pressure ( BP ) ,
triglycerides , FFA ,
insulin levels , and higher
high - density lipoprotein (
HDL )
cholesterol compared to
insulin - resistant
obese . -
pubmed
We found that the formation of yolk sac hemogenic endothelium and its hematopoietic potential were significantly impaired in the absence of
retinoic acid ( RA ) signaling , and could be restored in
RA - deficient (
Raldh2(-/- ) ) embryos by provision of exogenous
RA in utero . -
pubmed
BACKGROUND : Understanding how
androgen receptor (
AR ) function is modulated by exposure to
steroids , growth factors or small molecules can have important mechanistic implications for
AR - related disease therapies ( e.g. -
pubmed
This study compares
Breast Cancer 1 (
BRCA1 ) and
excision repair cross complementation group 1 (
ERCC1 ) expression as predictive markers and evaluates the in vitro enhancement of
platinum sensitivity using targeted agents in
sporadic ovarian cancer (
OC ) . -
pubmed
In summary , this study shows that low
BRCA1 and
ERCC1 expression correlate with improved survival in advanced
OC and
HDAC inhibition induces synergistic
cytotoxicity with
platinum in vitro . -
pubmed
Similar to the endocytosis motor
dynamin ,
Drp1 uses
GTP hydrolysis to power constriction of the outer mitochondrial membrane and ultimately mitochondrial division . -
pubmed
Dynamin phosphorylation in its unique C - terminal
proline - rich domain interferes with binding of accessory proteins that induce membrane curvature and inhibits
clathrin - mediated endocytosis . -
pubmed
We describe
brain calcifications secondary to
hypoparathyroidism in family members with
APS and compare clinical manifestations , the extent of
brain calcifications on
CT scans and the result of PET -
FDG scans . -
pubmed
The micelle properties of
PAsp ( OBzl )- b - PVP diblock copolymer were extensively studied by dynamic light scattering ( DLS ) , fluorescence spectroscopy , and transmission electron microscopy (
TEM ) . -
pubmed
Nucleotide binding by
Lhs1p is essential for its
nucleotide exchange activity and for function in vivo . -
pubmed
Both proteins have the characteristic domain structure of the
Hsp70 family consisting of a conserved N - terminal
nucleotide binding domain and a C - terminal substrate binding domain . -
pubmed
Kar2p is a canonical
Hsp70 whose substrate binding activity is regulated by cochaperones that promote either
ATP hydrolysis or
nucleotide exchange . -
pubmed
Lhs1p is a member of the
Grp170 /
Lhs1p subfamily of
Hsp70s and was previously shown to function as a
nucleotide exchange factor (
NEF ) for
Kar2p . -
pubmed
Analysis of the
nucleotide requirement of these functions demonstrates that
nucleotide binding to
Lhs1p stimulates the interaction with
Kar2p and is essential for
NEF activity . -
pubmed
In contrast ,
Lhs1p holdase activity is
nucleotide - independent and unaffected by mutations that interfere with
ATP binding and
NEF activity . -
pubmed
Thus ,
Lhs1p - dependent
nucleotide exchange activity is vital for
ER protein biogenesis in vivo . -
pubmed
In the carotenoid biosynthesis pathway ,
phytoene synthase (
PSY ,
EC 2.5.1.32 ) catalyzes the dimerization of two molecules of
geranylgeranyl pyrophosphate (
GGPP ) to
phytoene and has been shown to be a rate - limiting enzyme for the synthesis of carotenoids . -
pubmed
Transcript levels of carotenoid biosynthetic genes in the
CsPSY overexpressed plants remained unaltered except that
PDS and
ZDS showed a minor increase . -
pubmed
Decreasing
Mg ( 2 + ) ATPase activity confirms that the age - related loss of
PtdSer asymmetry is likely due to loss of
flippase activity , more prominent in
APP /
PS-1 brain . -
pubmed
Two - site sandwich ELISA on
SDS- and FA - soluble
APP /
PS-1 brain fractions were conducted to correlate
Abeta ( 1 - 40 ) and
Abeta ( 1 - 42 ) levels with age - related trends determined from the AV , NBD - PS , and
Mg ( 2 + ) ATPase assays . -
pubmed
ELISA revealed a significant increase in both
SDS- and FA - soluble
Abeta(1 - 40 ) and
Abeta ( 1 - 42 ) with age , consistent with
PtdSer and
flippase assay trends . -
pubmed
Lastly , because
PtdSer exposure is affected by pro - apoptotic
caspase-3 , levels of both latent and active forms were measured . -
pubmed
The model was used to fit the permeation profile of
lidocaine formulated in oil - in - water ( Type I ) and water - in - oil ( Type
II )
lecithin - linker microemulsions . -
pubmed
For Type
II systems , the increase in
lidocaine concentration in the skin was linked to the increase in skin - donor partition . -
pubmed
Donor - acceptor pairs of probes were attached to three site - directed di -
Cys mutants of
RLC , each having one
Cys at position 129 in the C - terminal lobe and the other at position 2 , 3 , or 7 in the N - terminal PD . -
pubmed
Protocol for
18F - FDG quantification in PET -
CT whole - body exams . -
pubmed
Soy
isoflavones did not affect
DBP [ -0.13 ( 95 % confidence interval -1.03 to 0.78 ) mmHg , P = 0.78 ; heterogeneity P = 0.20 , fixed effect model ] . -
pubmed
The induction of apoptosis by
Ca ( 2 +)-mobilizing agents results in
caspase activation , which is similar to what is seen with other inducers of apoptosis . -
pubmed
In addition ,
Ca ( 2 +)-dependent
proteases , such as
calpain and a
Ca ( 2 +)- dependent nuclear scaffold - associated serine protease , are also activated by
Ca ( 2 + ) signalling in some cell types where they appear to be involved in
alpha - fodrin and
lamin beta cleavage , respectively . -
pubmed
terreus the
cis - aconitate decarboxylase gene is part of an
itaconate acid related gene cluster including genes encoding two pathway specific transporters and a Zinc finger protein . -
pubmed
Cu ( II )-catalyzed
olefin migration and
Prins cyclization : highly diastereoselective synthesis of substituted
tetrahydropyrans . -
pubmed
Metal - ligand complexes of
Cu ( OTf )( 2 ) with an appropriate
bisphosphine ligand have been shown to effectively catalyze the formation of substituted
tetrahydropyrans via a sequential
olefin migration and
Prins - type cyclization . -
pubmed
3alpha - Hydroxysteroid dehydrogenase and related enzymes play important roles in the metabolism of endogenous compounds including
androgens ,
corticosteroid ,
prostaglandins and
bile acids , as well as drugs and xenobiotics such as
benzo ( a ) pyrene . -
pubmed
HT decisions included continued medical treatment ( 22 % ) , percutaneous coronary intervention ( PCI ; 22 % ) , coronary - artery bypass grafting ( CABG ; 34 % ) or further investigations such as pressure wire studies ,
dobutamine stress echo or cardiac magnetic resonance imaging ( 22 % ) . -
pubmed
In the present studies , the effects of oil rich in
EPA on gene expression and activation of
nuclear receptors was examined and compared with other
omega3 - PUFAs . -
pubmed
Serum
calcium level , fasting blood
glucose level ( FBG ) ,
hexokinase activity ,
glucose-6 - phosphatse and
fructose 1,6 bisphosphatase activity were measured to establish a relevant correlation between
vitamin D supplementation and
hyperglycemia in
T2DM . -
pubmed
The activity of the
glucose metabolic enzymes was also assayed in
diabetic group and was found to be deviated from control group ;
hexokinase ( 0.0241 + - 0.014 mug / mg / ml )
FBPase ( 0.433 + - 0.002 mug / mg / ml ) and
G6Pase ( 0.918 + - 0.02 mug / mg / ml ) . -
pubmed
However , the activity of these enzymes returned to near control values with
hexokinase activity reaching 0.717 + - 0.003 mug / mg / ml on
vitamin D3 supplementation . -
pubmed
Inhibitor and substrate interactions with
equilibrative nucleoside transporter 1 (
ENT1 ;
SLC29A1 ) are known to be affected by
cysteine - modifying reagents . -
pubmed
A previous study from our laboratory established
Cys222 in transmembrane (
TM ) 6 as the residue responsible for
methyl methanethiosulfonate ( a membrane - permeable
sulfhydryl modifier)-mediated enhancement of the binding of the
ENT1 inhibitor
nitrobenzylmercaptopurine riboside (
NBMPR ) in intact cells . -
pubmed
We thus hypothesized that the inhibitory effects of the modifiers were due to the one or more of the six
cysteine residues in the C - terminal half of
ENT1 , particularly one or both of those in the fifth intracellular loop (
Cys414 and
Cys416 ) . -
pubmed
[ Protective effects of
SUR2B /
Kir6.1 potassium channel opener
natakalim against
RAVECs injuries induced by
hypoxia ] . -
pubmed
The cell survival rate was determined by
MTT assay , con was measured using Griess Assay , RT - PCR was used to examine t he expression of
intercellular adhesion molecule-1 (
ICAM-1 ) ,
vascular endothelial growth factor (
VEGF ) ,
endothelin-1 (
ET-1 ) mRNA in RAVEC . -
pubmed
RESULTS :
Natakalim could reverse
hypoxia - induced changes in endothelial cell function , including increased endothelial cell survival rate and level of
NO concentration , significantly inhibited the
hypoxia - induced endothelial
ICAM-1 ,
ET-1 ,
VEGF mRNA expression levels increased . -
pubmed
CONCLUSION :
Natakalim have protective effects on
hypoxia - induced changes in endothelial cell function , increasing of permeation , excess expression of
cell adhesion molecules . -
pubmed
Attractor landscape analysis also suggested that combining
nutlin-3 with inhibition of
Wip1 would synergize to stimulate a sustained increase in
p53 activity and promote
p53 - mediated cell
death . -
pubmed
ZO-2 departure from the nucleus requires intact S257 , and stabilizing the beta - O - linked N - acetylglucosylation (
O - GlcNAc ) of S257 with
O -( 2 - acetamido-2 - deoxy - d - glucopyranosylidene ) amino - N - phenylcarbamate , an inhibitor of
O - GlcNAcase , triggers nuclear exportation and proteosomal degradation of
ZO-2 . -
pubmed
At the plasma membrane
ZO-2 is not
O - GlcNAc , and instead , as TJs mature , it becomes phosphorylated at S257 by
protein kinase Czeta . -
pubmed
Absorption of
arginine - modified nanohydroxyapatite particles ( Arg - nHAP ) and
DZ1 (
latent membrane protein 1 [
LMP1]-targeted ) reached nearly 100 % efficiency under in vitro conditions . -
pubmed
Phorbol 12 - myristate 13 - acetate (
PMA ) , the most commonly used
phorbol ester , binds to and activates
protein kinase C (
PKC ) , causing a wide range of effects in cells and tissues . -
pubmed
PMA induced the expression of c -
fos gene transcription and protein expression , and led to activation of the
MAPK pathways in
NP cells . -
pubmed
The c - fos promoter was suppressed completely in the presence of the
MAPK inhibitor
PD98059 , an inhibitor of the
MEK /
ERK kinase cascade , but not in the presence of
SKF86002 ,
SB202190 , or
SP600125 . -
pubmed
Prior blockade of
beta3 adrenoceptors (
SR59230A 10 mg / kg ip ) significantly reduced the amplitude of each temperature increase . -
pubmed
Membrane stabilizing agents , which include the anticonvulsants , antiarrhythmics and probably
corticosteroids , may act by blocking
sodium channels on damaged neural membranes . -
pubmed
Prescriptions for long - term management varied among districts , as follows : inhaled
corticosteroids ( ICS ) , mean of 37.3 % ( 0 - 66.7 % ) ; ICS / long - acting beta - agonists ( LABA ) , 27.9 % ( 16.7 - 66.7 % ) ; and
leukotriene receptor antagonist ( LTRA ) , 20.5 % ( 0 - 50.0 % ) CONCLUSION : These findings suggest that
asthma treatment in emergency settings varies by facility and region . -
pubmed
At the age of 4 mo , he was diagnosed as having
CNDI , judging from
fever with
hypernatremia ( serum
Na 153 mEq / L ) , diluted urine ( urine osmolarity 193 mOsm / kg ) , high
antidiuretic hormone (
plasma antidiuretic hormone 53 pg / mL ) , and normal renal function ( serum
creatinine 0.3 mg / dL ) . -
pubmed
Pre - adolescents in " dry " communities ( with laws restricting
alcohol use ) had much higher lifetime and past 30 - day
HLP use . -
pubmed
The data produced indicated that astroglial cells contain
P450 - reductase which transforms
paraquat into a
pyridium free - radical . -
pubmed
The effect of
simvastatin on inflammatory
cytokines in community - acquired
pneumonia : a randomised , double - blind , placebo - controlled trial . -
pubmed
The secondary end points were serum concentrations of inflammatory
cytokines and partial pressure of arterial
oxygen / fractional inspired
oxygen ( PaO2 / FiO2 ) at 48 h after treatment administration . -
pubmed
No significant differences in PaO2 / FiO2 ( p=0.37 ) ,
C reactive protein ( p=0.23 ) ,
tumour necrosis factor - alpha ( p=0.58 ) ,
interleukin 6 (
IL-6 ; p=0.64 ) , and
IL-10 ( p=0.61 ) levels at 48 h of hospitalisation were found between
simvastatin and placebo groups . -
pubmed
In this work , we determined the reference intervals ( RIs ) for total
cholesterol (
TC ) ,
high - density lipoprotein cholesterol (
HDL - c ) , non -
high - density lipoprotein cholesterol (
nHDL - c ) ,
low - density lipoprotein cholesterol (
LDL - c ) and
triglycerides (
TG ) in Brazilian healthy children and adolescents . -
pubmed
For the
LDL -
cholesterol , the values corresponding to the percentiles above , aged 1 to 8 years and 9 to 12 years , were 132 mg / dL 139 mg / dL , respectively . -
pubmed
Design , synthesis and docking studies of novel
thienopyrimidine derivatives bearing
chromone moiety as
mTOR /
PI3Kalpha inhibitors . -
pubmed
Bisulfite sequencing evaluated DNA methylation of placenta - specific 8 (
PLAC8 ) . -
pubmed
Methylation status of 17
CpG sites in
PLAC8 negatively correlated with mRNA expression . -
pubmed
Following the recommendations of the WHO , Cameroon changed its
malaria treatment policy from monotherapy to
artemisinin - based combination therapy (
ACT ) as the first - line treatment for
uncomplicated malaria . -
pubmed
The anti - malarials prescribed were Anti -
malaria combination therapy (
ACT ) - 1216 ( 47.6 % ) ,
quinine 1044 ( 40.8 % ) or
SP 296 ( 11.6 % ) . -
pubmed
Although there is an overall increase in the prescription of
ACT , less prescription of
quinine and a noticeable restrain from prescription of SP to febrile cases , the old practice was still rampant . -
pubmed
OBJECTIVE : To preliminarily develop a surfactant - free , polymeric solid dispersion (
PSD ) of
paclitaxel suitable for oral administration . -
pubmed
The absolute oral bioavailability ( AOB , 20 mg / kg ) of the three formulas [
HPMCAS /
paclitaxel / WCB = 4/1/0 ( F1 ) , 8/1/0 (
F2 ) and 4/1/4 (
F3 ) , w / w / w ] were 11.8 , 13.6 and 25.6 % , respectively . -
pubmed
The AOB of
F3 is nearly seven times higher than that ( 3.8 % ) of
paclitaxel material ( a control ) . -
pubmed
The advantage of higher HPMCAS /
paclitaxel ratio of
F2 in a dissolution test was not reflected in the first in vivo test due to the relatively higher dose of polymer which could not be effectively dissolved under the limitation of intestinal environment . -
pubmed
Further characterization of the effect of
ethanol on
voltage - gated Ca ( 2 + ) channel function in developing
CA3 hippocampal pyramidal neurons . -
pubmed
We previously reported that acute and repeated exposure to
ethanol during the third trimester - equivalent inhibits long - term potentiation of
GABAA receptor - dependent synaptic currents in
CA3 pyramidal neurons through a mechanism that depends on retrograde release of brain - derived
neurotrophic factor driven by activation of voltage - gated
Ca ( 2 + ) channels ( Zucca and Valenzuela , 2010 ) . -
pubmed
Here , we further investigated the acute effect of
ethanol on the function of voltage - gated
Ca ( 2 + ) channels in
CA3 pyramidal neurons using
Ca ( 2 + ) imaging techniques . -
pubmed
To investigate the long - term consequences of
ethanol on voltage - gated
Ca ( 2 + ) channels , we used patch -
clamp electrophysiological techniques to assess the function of
L - type voltage - gated Ca ( 2 + ) channels during and following ten days of vapor
ethanol exposure . -
pubmed
Taken together with our previous findings , our results suggest that 3(rd ) trimester - equivalent
ethanol exposure transiently inhibits L - type voltage - gated
Ca ( 2 + ) channel function in
CA3 pyramidal neurons and that compensatory mechanisms restore their function during
ethanol withdrawal . -
pubmed
Assessment of the performance of the continuous improvement programmes based on six measures comprising the
post - caesarean rehabilitation score : duration infusion , early oral analgesia , time to removal of the urinary catheter , time to return to drinking , eating recovery time , use of
carbetocin . -
pubmed
Intact mitochondrial
Ca2 + uniport is essential for agonist - induced activation of
endothelial nitric oxide synthase (
eNOS ) . -
pubmed
The knock - down of
mitochondrial calcium uptake 1 (
MICU1 ) , a gatekeeper of the
MCU , increased both mitochondrial
Ca2 + sequestration and
Ca2 + -induced
NO signals . -
pubmed
The positive correlation between mitochondrial
Ca2 + elevation and
NO production was independent of
eNOS phosphorylation at
serine1177 . -
pubmed
Our findings emphasize that manipulating mitochondrial
Ca2 + uptake may represent a novel strategy to control
eNOS - mediated
NO production . -
pubmed
Corrigendum to " GENEDIA multi
influenza Ag rapid test for detection and
H1 , H3 , and
H5 subtyping of
influenza viruses " [ J. Clin . -
pubmed
Several
kinases and
phosphatases have been shown to be crucial for
ABA - dependent regulation of the ion transporters . -
pubmed
To date , the
Ca2 + -dependent regulation of Shaker channels by
Ca2 + - dependent protein kinases (
CPKs ) is still elusive . -
pubmed
Furthermore ,
Ca2 + -induced stomatal closure is impaired in two
cpk33 mutant plants . -
pubmed
Multicellular spheroids can be achieved within 24 h and further boosted by incorporating
collagen fibrils in
PDMS - HDA . -
pubmed
For laboratory evaluation ,
cholestasis parameters (
bilirubin ,
gamma - GT ) , liver cell parameters (
ASAT ,
ALAT ) and liver synthesis parameters ( quick ,
albumin ) were determined . -
pubmed
RESULTS : In chemical laboratory and histological evaluation , a distinction between acute and chronic
cholestatic liver injury with identification of
inflammation and regeneration could be demonstrated by an increase in
cholestasis (
bilirubin : 1 - wk group , 156.83 + - 34.12 mumol / L , P = 0.004 ) and liver cell parameters (
ASAT : 2 - wk group , 2.1 + -
2.19 mumol / L.s , P = 0.03 ;
ALAT : 2 - wk group , 1.03 + - 0.38 mumol / L.s , P = 0.03 ) after bile duct ligation ( BDL ) . -
pubmed
The following anthroponetric parameters were determined :
body mass index (
BMI ) , waist to hip ratio , blood lipid profile ( total
cholesterol ,
triglycerides ,
high density lipoproteins (
HDL ) , and
low density lipoproteins (
LDL ) ) . -
pubmed
Leptin and
insulin levels were measured using com- mercial ELISA kits & lt;<Leptin ELISA>> ; and & lt;<
Insulin ELISA>> ; ( DRG International , Inc. , USA ) respectively
Insulin resistance in-
dex HOMA -JR ( Homeostasis Model Assessment of
Insulin Resistance ) was calculated . -
pubmed
Signficant direct correlations between
leptin blood levels and
BMI , HOMA - IR index ,
triglycerides , and
cholesterol were documented The relationships between blood levels of
leptin and the thickness of the gallbladder ( GB ) wall , the amount of
cholesterol crystals in bile , and decreased bile release rate from GB which suggests effect of
leptin on the structural and finctional changes in GB . -
pubmed
To improve cellular uptake and transport of pure nanodrug in cells , here , a lipid covered
saquinavir (
SQV ) pure drug
NP ( Lipo@nanodrug ) was designed by modifying a pure
SQV NP ( nanodrug ) with a phospholipid bilayer . -
pubmed
Although the exact mechanism of the development of
ovarian cysts is unknown , it is thought that increased
androgen levels stimulate folicle development by increasing
follicle stimulating hormone (
FSH ) levels . -
pubmed
Laboratory test results were as follows :
sodium : 126 mEq / L ,
potassium : 5.4 mEq / L ,
renin : 132 pg / mL ,
adrenocorticotropic hormone (
ACTH ): 207 pg / mL ,
cortisole : 7.8 mug / dL , basal
17OH progesterone : 21 ng / mL ,
androstenedione : 5.1 ng / mL ,
testosterone : 1188 ng / dL and
dehydroepiandrosterone sulfate (
DHEAS)>1500 mug / dL. -
pubmed
CONCLUSIONS : The findings in our patient suggest that a decline in adrenal
androgens after glucocorticoid treatment resulted in an increase in
gonadotropin levels and the giant
cyst is developed by activation of
gonadotropin cascade and increased
gonadotropin receptors , instead of
androgens . -
pubmed
Our results also supported that more aerial
nodal roots and fewer crown roots might be favored in temperate maize , and this root architecture might efficiently improve root - lodging resistance and the ability to take up deep water and
nitrogen under dense planting . -
pubmed
Cadmium disrupts signaling of the
hypoxia - inducible (
HIF ) and
transforming growth factor ( TGF - beta ) pathways in placental JEG-3 trophoblast cells via reactive
oxygen species . -
pubmed
The induction of cytosolic reactive
oxygen species ( ROS ) , expression of
metallothionein (
MT ) isoforms ,
HIF1alpha and downstream targets , and expression of
TGFbeta pathway - associated genes and proteins were assessed . -
pubmed
Additionally , the effect of pre - treatment with the antioxidant
N - acetyl cysteine (
NAC ) on ROS generation and effects on
HIF ,
MT and
TGF - beta signaling pathways was examined . -
pubmed
Cd and
Cd - mixture treated cells displayed higher levels of ROSs with accompanying disruption of
HIF and
TGFbeta pathway signaling versus controls , with the
Cd - mixture eliciting a greater effect . -
pubmed
Conversely , pretreatment with
NAC reduced
Cd - induced ROS production and disruption of
HIF ,
MT and
TGFbeta pathway signaling . -
pubmed
The plasmonic - photonic hybrid mode between the
Au nanoparticles (
NP ) and the
diatomite layer could supply nearly 10 times the increment of SERS signal ( MBA ) intensity compared to the common
silica gel chromatography plate . -
pubmed
The results indicated that the recombinant 140
amino acid protein , designated
rMPN668 , displayed
hydroperoxidase activity towards both organic (
tert - butyl hydroperoxide ) and inorganic (
hydrogen peroxide )
hydroperoxides in the presence of a reducing agent such as
dithiothreitol . -
pubmed
Additionally , homology modeling of
MPN668 and a molecular dynamics simulation suggest that both
Cys55 and
Cys119 form part of the active site of the protein . -
pubmed
Mutants in which
Cys55 or
Cys119 were replaced with a
serine lack antioxidant activity , indicating that
MPN668 is a
Cys - based
peroxidase , consistent with it representing a new member of the
Ohr family . -
pubmed
CYP2D6 genotype and
endoxifen plasma concentration do not predict
hot flash severity during
tamoxifen therapy . -
pubmed
Tamoxifen is a prodrug and requires bioactivation by
CYP2D6 . -
pubmed
There is paucity of prospectively collected data in terms of
CYP2D6 genotype and measured
tamoxifen ,
4 - hydroxytamoxifen and
endoxifen concentrations in relation to
hot flash severity during
tamoxifen therapy . -
pubmed
Plasma concentrations of
tamoxifen ,
4 - hydroxytamoxifen , and
endoxifen were measured using liquid chromatography - tandem mass spectrometry and genotyping was carried out for
CYP2D6 . -
pubmed
CONCLUSIONS :
Hot flash severity during
tamoxifen therapy can not be accounted for by
CYP2D6 genotype or observed plasma concentration of
tamoxifen ,
4 - hydroxytamoxifen , or
endoxifen . -
pubmed
Inhibition of the
sodium -- glucosecotransporter-2 (
SGLT2 ) can increase urinary
glucose excretion and decrease plasma
glucose levels in an
insulin - independent manner . -
pubmed
Advantages of
ertugliflozin include reduction in glycated
hemoglobin ,
weight loss and blood pressure lowering with a low risk of
hypoglycemia . -
pubmed
Synthesis , characterization , and
SAR of
arylated indenoquinoline - based
cholinesterase and
carbonic anhydrase inhibitors . -
pubmed
Monophenyl ( 16 - 18 ) indenoquinolines significantly inhibited the
AChE and
BChE enzymes in ranges of IC50 37 - 57 nM and 84 - 93 nM , respectively , compared with their starting materials 15a - c and reference compounds (
galanthamine and
tacrine ) . -
pubmed
On the other hand , these novel arylated
indenoquinoline - based derivatives were effective inhibitors of the
BChE ,
hCA I and II ,
BChE and
AChE enzymes with Ki values in the range of 37 + - 2.04 to 88640 + - 1990 nM for
AChE , 120.94 + - 37.06 to 1150.95 + - 304.48 nM for
hCA I , 267.58 + - 98.05 to 1568.16 + - 438.67 nM for
hCA II , and 84 + - 3.86 to 144120 + - 2910 nM for
BChE. -
pubmed
As a result ,
monophenyl indenoquinolines 16 - 18 may have promising anti -
Alzheimer drug potential and
3,8 - dibromoindenoquinoline amine ( 15f ) can be novel
hCA I and
hCA II enzyme inhibitors . -
pubmed
25 - Hydroxyvitamin D (
25 ( OH ) D ) , fasting plasma
glucose and
insulin were measured . -
pubmed
The Food and Drug Administration ( FDA or we ) is classifying the high flow humidified
oxygen delivery device into class
II ( special controls ) . -
pubmed
BACKGROUND : We conducted a single - arm phase II trial to evaluate the efficacy and adverse effects ( AEs ) of an anti -
epidermal growth factor receptor monoclonal antibody ,
nimotuzumab , combined with
cisplatin and
5 - fluorouracil ( PF ) as first - line treatment in recurrent metastatic
nasopharyngeal carcinoma after radical radiotherapy . -
pubmed
PGE2 and a selective
EP3 agonist suppressed
cytokine production and expression induced by
polyinosine - polycytidylic acid stimulation , such as
chemokine ligand 5 and
chemokine motif ligand 10 . -
pubmed
Here , we describe a novel liposome - based subunit vaccine formulation for
tuberculosis (
TB ) based on
phosphatidylserine encapsulating two prominent TB antigens ,
Ag85B , and
ESAT-6 . -
pubmed
An increasing initial osmotic pressure within the mineral medium decreases both
glucose release and
protease yield . -
pubmed
This work presents the preparation of cross - linking
Au nanoparticle (
NP ) monolayer membranes by the
thiol exchange reaction and their enhanced mechanical properties . -
pubmed
Moreover , the modulus of the cross - linking monolayer was measured by atomic force microscopy (
AFM ) and the result showed that the membrane with a longer
dithiol molecule had a larger modulus , which might derive from the unbroken and intact structure of the cross - linking monolayer due to the selected appropriately lengthed
dithiol . -
pubmed
Data suggest that imbalance of metals , including
copper ,
zinc ,
iron , and
manganese , induces abnormalities in processing and degradation of
prion proteins that are accompanied by self - propagation of PrPSc . -
pubmed
Post - synthesis strategy to integrate
porphyrinic metal - organic frameworks with
CuS NPs for synergistic enhanced photo - therapy . -
pubmed
Porphyrinic metal - organic frameworks with
CuS NPs are obtained in aqueous solution via a simple
post - synthesis strategy . -
pubmed
EPR and magnetic susceptibility measurements helped us to assign the oxidation state of the
manganese complexes in their native state . -
pubmed
Preparation of
theasinensin A and
theasinensin B and exploration of their inhibitory mechanism on
alpha - glucosidase . -
pubmed
In the present study ,
TSA and
TSB were chemically synthesized and purified , and their inhibitory effects on
alpha - glucosidase and potential mechanisms were investigated . -
pubmed
The results showed that
TSA and
TSB could inhibit the activity of
alpha - glucosidase in a reversible and noncompetitive manner with IC50 values of 6.342 and 24.464 mug mL-1 , respectively , which were much lower than that of
acarbose . -
pubmed
The fluorescence and circular dichroism spectra revealed that
TSA and
TSB could alter the microenvironment and the secondary structure of
alpha - glucosidase , thereby decreasing the
alpha - glucosidase activity . -
pubmed
Molecular docking results indicated that both
TSA and
TSB had a strong binding affinity to
alpha - glucosidase by hydrophobic interactions and
hydrogen bonds . -
pubmed
Moreover , the stronger inhibition of
TSA on
alpha - glucosidase might be related to the closer binding site to the active site pocket of
alpha - glucosidase . -
pubmed
Oral
prednisolone was administered on day 33 then
fever subsided with dramatically clinical improvement ( decreased
CRP and resolution of lung consolidation ) . -
pubmed
RES treatment increased p -
AMPK alpha /
AMPK alpha and p -
ULK1 S777 /
ULK1 and the autophagy related proteins (
Beclin1 ,
LC3 II / I ) and its effects on TC and improvement in
insulin resistence were quenched by the inhibitor of autophagy , 3 - MA . -
pubmed
Together , these results suggest that the effect of
RES in treatment of
DN may involve
AMPK alpha /
mTOR - mediated autophagy . -
pubmed
The
dopamine transporter SPECT with
123I ioflupane showed abnormal scans in bilateral striatum . -
pubmed
High levels of
sPD-1 or
sPDL1 were not correlated to PFS under bevacizumab while they were independent prognostic factors of PFS in the
sunitinib group . -
pubmed
In this study , response surface methodology ( RSM ) and box - behnken design ( BBD ) are used to investigate the stability of fiber in polymeric base suspensions , including carboxy methyl cellulose ( CMC ) ,
polyacrylamide (
PAM ) , and xanthan gum (
XG ) . -
pubmed
Evaluation of a possible role of
NaK - ATPase in transtubular
sodium reabsorption is difficult , since
ouabain doses that inhibit this enzyme system in vitro completely [ 1 , 11 ] , can not be applied in vivo . -
pubmed
However , in this model , supramaximal doses of
ouabain as used to block
NaK - ATPase completely induced a potent vasoconstriction , which lowers the filtered load of
sodium . -
pubmed
In the following experiments we reinvestigated the relationship between inhibition of renal
NaK - ATPase and the reduction of
Na - reabsorption by
ouabain under more appropriate conditions . -
pubmed
TGF beta 1 mRNA , but not TGF
beta 2 or TGF beta 3 mRNA , was also produced constitutively by all four cell lines . -
pubmed
Quantitation by bioassay revealed baseline
TGF beta secretion of approximately 1 ng/10(6 ) cells over 48 h. Stimulation of
mastocytoma cells with
phorbol ester increased the rate of release of
TGF beta 1 , most markedly in the first 30 min after stimulation , without increasing TGF
beta 1 mRNA . -
pubmed
It is suggested that
apo E secreted from peripheral tissues enhances the uptake of lipoproteins by themselves or by surrounding cells in the local environment which demand
cholesterol and express
LDL receptors . -
pubmed
Post - marketing surveillance of
quinolones 1988 - 1990 . -
pubmed
It has been much easier to obtain original data on
adverse drug reactions (
ADR ) of
quinolones from the pharmaceutical industry than it was two years ago . -
pubmed
This is to be welcomed and , as anticipated , the new data continue to suggest that the new
4 - quinolones have an
ADR profile which is very similar to that of other antimicrobials . -
pubmed
Visual disturbance is not a prominent feature , in contrast to the
ADR profile of
nalidixic acid . -
pubmed
After VF , tissue concentrations of
ATP ,
AMP ,
creatinine phosphate (
CP ) , and
lactate were measured . -
pubmed
After NE , but not E , tissue
AMP concentrations were significantly increased , and
CP concentrations were reduced compared with vehicle ( P less than 0.05 ) . -
pubmed
Tissue levels of
adenosine triphosphate ( a major tissue energy store ) ,
superoxide dismutase ( a major defense against free radicals ) ,
xanthine oxidase ( an enzymatic source of free radicals ) , and
edema were measured . -
pubmed
Photoaffinity labelling of
dopamine D2 receptors by
[ 3H ] azidomethylspiperone . -
pubmed
We have characterized the
dopamine D2 receptor photoaffinity probe ,
[ 3H ] azido - N - methylspiperone (
[ 3H ] AMS ) . -
pubmed
However , no direct correlation between the increasing sensitivity to
antigen and the decreasing reactivity to
IL2 appears to exist . -
pubmed
In
TAM from all
tumors studied the expression of
PA was markedly greater than that of resident peritoneal macrophages . -
pubmed
The fact that
PA was similarly enhanced in peritoneal macrophages responding to a standard stimulation (
thioglycolate ) and in
TAM suggests that the expression of
PA is part of a more general cellular inflammatory response to
injury . -
pubmed
Basal serum
luteinizing hormone (
LH ) concentration was increased by
NAL during each trial in the luteal phase and
LH pulse amplitude was proportionately increased by 158 % , 164 % and 350 % during the early luteal , mid luteal and early follicular phases , respectively . -
pubmed
The apparent
NAL induced increase ( 92 % ) in
LH pulse amplitude during the late luteal phase was not significant . -
pubmed
NAL only affected
LH pulse frequency during the early follicular phase , when it was decreased . -
pubmed
Mean serum
follicle stimulating hormone (
FSH ) concentration was not affected by
NAL . -
pubmed
The results of this study indicate that endogenous opioid peptides ( EOPs ) may partially mediate the suppressive influence of
estradiol-17 beta (
E2 ) on
LH pulse amplitude and also the stimulatory effect of
E2 on
LH pulse frequency in the early follicular phase . -
pubmed
The data may suggest that
NAL enhances the amplitude of
pulses of
gonadotropin releasing hormone (
GnRH ) by counteracting
E2 inhibitory effects on
LH release at the level of the pituitary . -
pubmed
This report describes the initial characterization of small muscle - associated connective tissue proteoglycans synthesized in embryonic chick leg in
ovo and by embryonic leg muscle - associated secondary fibroblasts in vitro by
CsCl equilibrium density gradient centrifugation . -
pubmed
Formation of mixed
disulfide adducts at
cysteine-281 of the
lactose repressor protein affects operator and inducer binding parameters . -
pubmed
The
lactose repressor protein has been modified with three
sulfhydryl - specific reagents which form mixed
disulfide adducts . -
pubmed
Modification of the
lac repressor with
didanylcystine decreased operator binding only 4 - fold , and nonspecific DNA binding increased 3 - fold compared to unmodified repressor . -
pubmed
Nuclear magnetic resonance studies of protein binding sites : the complex of
staphylococcal nuclease with
3 ' , 5 '- thymidine diphosphate . -
pubmed
the biosynthetic pathway (
17 beta - HSD ,
delta 5 - 3 beta - HSD ) leading towards the
androgens was enhanced in the abdominal skin . -
pubmed
The
lithium clearance technique has been proposed as a non - invasive method whereby fluid delivery from the pars recta and pars convoluta of proximal tubules can be measured as CLi and
CIN [ 0.78 CLi /
CIN + 0.22 ] , respectively [ 12 ] , CLi being the clearance of
lithium and
CIN that of
inulin . -
pubmed
Analysis of
cyclosporin interactions with
antibodies and
cyclophilin using the BIAcore . -
pubmed
The immunosuppressive cyclic undecapeptide
cyclosporin A (
CS ) exists in various conformers in water . -
pubmed
We conclude that some patients with
PPS have in the serum high titers of
IgM anti - PV antibodies , implying an ongoing antibody response to a viral
antigen . -
pubmed
We have previously identified six mutations in the
G6Pase gene of
glycogen storage disease type 1a patients and demonstrated that these mutations abolished or greatly reduced enzymatic activity of
G6Pase , a hydrophobic protein of 357
amino acids . -
pubmed
To further understand the roles and structural requirements of
amino acids 83 , 222 , 295 , and those at the
carboxyl terminus in
G6Pase catalysis , we characterized mutant
G6Pases generated by near - saturation mutagenesis of the aforementioned
amino acids . -
pubmed
Substitution of
Arg-83 with
amino acids of diverse structures including
Lys , a conservative change , yielded mutant
G6Pase with no enzymatic activity . -
pubmed
All other substitutions of
Arg-295 had no
G6Pase activity , suggesting that the role of
Arg-295 is to stabilize the protein either by salt bridge or
hydrogen - bond formation . -
pubmed
It is possible that
Arg-83 is involved in stabilizing the enzyme (
His)-
phosphate intermediate formed during
G6Pase catalysis . -
pubmed
Substitution of
His-119 with
amino acids of diverse structures also yielded mutant
G6Pase with no activity , suggesting that
His-119 is the
phosphate acceptor in
G6Pase catalysis . -
pubmed
Familial high serum concentrations of the
carboxyl - terminal propeptide of
type I procollagen . -
pubmed
We describe a family with an apparently autosomal - dominant trait that caused extremely high circulating concentrations of the
carboxyl - terminal propeptide of
type I procollagen (
PICP ) . -
pubmed
Furthermore , their serum concentrations of the
amino - terminal propeptide of
type I procollagen (
PINP ) were normal . -
pubmed
Amino acid analysis , mass determination ( 2,165 Da ) and N - terminal sequencing (
Ile - Ala - Xaa - Lys - Phe - Ile - Xaa - Xaa - Pro - Gly - Xaa - Ala - Lys - block ) demonstrated that staphylococcin 1580 is identical to
epidermin , a
lanthionine - containing antibiotic peptide ( lantibiotic ) . -
pubmed
The data support a model in which the state of correlation of pre- and
post - synaptic activity is converted by the voltage - dependent
NMDA receptor channel into a graded postsynaptic
Ca2 + signal . -
pubmed
Another group of neurotransmitters , including
5 - hydroxytryptamine ( 5 -
HT ) ,
thyrotropin - releasing hormone (
TRH ) and
gamma - aminobutyric acid (
GABA ) was never expressed in situ in DRG neurons . -
pubmed
Similar effects were observed when spermatozoa were incubated with
phosphodiesterase inhibitors or cell - permeant
cAMP analogs , suggesting that
cAMP is involved in these two processes . -
pubmed
The inhibition of these phenomena by
cAMP - dependent protein kinase inhibitors , and the stimulation by protein phosphatase inhibitors , suggest that
Ser /
Thr protein phosphorylation plays an important role in the regulation of both sperm capacitation and protein -
tyrosine phosphorylation pathways . -
pubmed
However , observations that both
calyculin A and
okadaic acid stimulated sperm capacitation , whereas only
calyculin A increased
p105 and
p81 phosphotyrosine content and sperm velocity , suggest that
protein phosphatase PP1 is involved in the two latter phenomena while
PP2A mediates sperm capacitation . -
pubmed
These results suggest that divergent pathways might regulate
tyrosine phosphorylation of
p105 and
p81 and sperm capacitation after
cAMP - dependent phosphorylation of an intermediate protein . -
pubmed
These findings were basically consistent with those in apoptosis induced by a
monoclonal antibody against the surface
antigen Fas . -
pubmed
Expression patterns of brush - border
antigen as well as molecular markers of kidney differentiation
Wt1 ,
Lim1 ,
Hgf and
c - met ,
c - ret ,
Shh ,
Wnt4 ,
Wnt7b , and
Wnt11 were analyzed in explants by whole mount and tissue section in situ hybridization following 1 - 9 days in culture . -
pubmed
Mycobacterial cell wall components were assessed by ELISA for their ability to bind the
carbohydrate recognition domain of
rMBP . -
pubmed
The best ligand was
mannosyl - lipoarabinomannan , followed by
lipomannan ,
phosphatidylinositol mannoside ,
arabinosyl - lipoarabinomannan , and
dimycolated trehalose (
cord factor ) . -
pubmed
rMBP did not bind to partially purified lipid fractions containing
glycopeptidolipids . -
pubmed
Previous studies have shown that adrenalectomy results in a small increase in hippocampal
alpha - bungarotoxin binding , whereas seven days of chronic treatment with high doses of
corticosterone results in decreases in
alpha - bungarotoxin binding in several brain regions . -
pubmed
The studies reported here examined the effects of different doses of
corticosterone on brain
alpha - bungarotoxin binding . -
pubmed
These results argue that
corticosterone levels play an important role in modulating the level of the
brain nicotinic receptors that bind
alpha - bungarotoxin with high affinity . -
pubmed
Complementation analysis with 15 reference single - site loci ( cistrons ) in the zeste - white region of the X chromosome revealed that 29 of 723
urethane - induced recessive lethals were located in the
zeste - white region and all were restricted to a single locus . -
pubmed
Positron emission tomography - based
boron neutron capture therapy using
boronophenylalanine for high - grade
gliomas : part
II . -
pubmed
In the Born test
4 - chlorophenylethenecarboxamidoxime ( 21 ) was most active and inhibited the blood
platelet aggregation induced by
collagen with an IC50 = 3 microM. -
pubmed
The
THF extracts were derivatized with
tert - butyldimethylsilyl (
t - BDMS ) for
GC -
MS analysis . -
pubmed
A single crystal separated from the extract was used for an x - ray structure analysis , giving the monoclinic unit cell , space group
P21 / c with ao = 5.543 A , bo = 8.845 A , co = 5.086 A , and beta = 91.9 degrees , consistent with
beta - succinic acid ,
HOOC ( CH2 ) COOH . -
pubmed
We determined the affinities of eight novel
histamine H ( 3 )- receptor ligands (
ethers and
carbamates ) for
H ( 3 )- receptor binding sites and their agonistic / antagonistic effects in two functional
H ( 3 )- receptor models . -
pubmed
Only
FUB 373 and 407 inhibited the evoked overflow by themselves ( intrinsic activities 0.3 and 0.4 ) ; these effects were counteracted by the
H ( 3 )- receptor antagonist
clobenpropit . -
pubmed
In conclusion , the novel compounds are partial
H ( 3 )- receptor agonists (
FUB 373 and 407 ) or
H ( 3 )- receptor antagonists ; comparison with
FUB 335 shows that the transition from antagonist to agonist is caused by a slight structural change . -
pubmed
They had no effect on the cell influx , whereas the
A3 antagonist ,
MRS-1220 , significantly inhibited cellular infiltration , suggesting mediation through
A3 receptors . -
pubmed
CONCLUSIONS :
5 '- AMP caused a rapid migration of eosinophils and macrophages into the airways only in sensitized guinea - pigs , and this was blocked by the
A3 antagonist
MRS-1220 . -
pubmed
This
adhI mutant expressed high levels of a novel
alcohol dehydrogenase (
AdhI ) that uses
ethanol ,
1 - propanol and
2 - propanol as substrates . -
pubmed
Second trimester maternal serum
alpha - fetoprotein (
MS - AFP ) ,
human chorionic gonadotrophin (
hCG ) ,
unconjugated estiol (
uE3 ) , and
inhibin - A (
INH - A ) levels were evaluated in pregnancies complicated by
triploidy . -
pubmed
However , there were the characteristic decreases in plasma
atrial natriuretic peptide concentrations , and increases in plasma and urinary
cortisol . -
pubmed
Atrial and ventricular signals were recorded during
sinus rhythm (
SR ) ,
atrial flutter ,
AF , and
VT or
VF . -
pubmed
During VF atrial "
sinus " signals had significantly ( P & lt ; 0.01 ) lower amplitudes ( 1.4 + /- 0.52 mV ) than during
SR . -
pubmed
Augmented expression of
P - gp / multi - drug resistance gene by
all - trans retinoic acid in monocytic leukemic cells . -
pubmed
By liquid culture of
leukemia cell lines and fresh
AML cells with the addition of
all - trans retinoic acid (
ATRA ) , modulation of
P - gp /
MDR1 and
Egr1 was observed and the pattern of modulation was divided into four groups : ( 1 ) blastic
AML type , in which distinct expression of
P - gp /
MDR1 and
CD34 was not influenced by
ATRA ; ( 2 ) t(8;21)
AML type , in which P - gp /
MDR1 expression was augmented by
ATRA , while
CD34 was kept high ; ( 3 )
AML M3 type , in which P - gp /
MDR1 expression was reduced with granulocytic differentiation by
ATRA ; ( 4 ) monocytic
AML type , in which P - gp /
MDR1 expression was augmented by
ATRA , while
CD34 expression decreased , and strong
Egr1 expression was downregulated just prior to the augmentation of P - gp /
MDR1 expression . -
pubmed
WT1 expression was not influenced by the addition of
ATRA in each group . -
pubmed
However , P - gp /
MDR1 expression augmented by
ATRA , which was observed in monocytic
AML , was recognized as a functional molecule of mature monocyte / macrophage , because
CD34 expression decreased and
CD13 expression increased by
ATRA . -
pubmed
Finally , expression of
P - gp /
MDR1 in
monocytic leukemia , which was functionally confirmed by
Rh123 efflux study , was thought to be closely related to the characteristic modulation of
Egr1 expression by
ATRA . -
pubmed
Membrane - bound
transhydrogenases are conformationally driven proton - pumps which couple an inward proton translocation to the reversible reduction of
NADP+ by
NADH ( forward reaction ) . -
pubmed
The
2 - or 4 - hydroxypyrimidine - linked nitroreductase activity was inhibited by
oxypurinol and
( + /-)- 8 -( 3 - methoxy-4 - phenylsulfinylphenyl ) pyrazolo [ 1,5 - a ]- 1,3,5 - triazine-4 ( 1H )- one (
BOF-4272 ) , inhibitors of
xanthine oxidase , but not by
menadione ,
chlorpromazine and
isovanillin , inhibitors of
aldehyde oxidase . -
pubmed
When skin cytosol was applied to a
DEAE - cellulose column , the fractions containing
xanthine oxidase exhibited a marked
2 - hydroxypyrimidine - linked nitroreductase activity . -
pubmed
Moreover , the
xanthine oxidase fraction exhibited a significant
nitroreductase activity in the presence of
2 - hydroxypyrimidine ,
4 - hydroxypyrimidine or
hypoxanthine , and these activities were inhibited by inhibitors of
xanthine oxidase . -
pubmed
These results indicated that reduction of
2 - nitrofluorene in the skin was mainly catalyzed by
xanthine oxidase . -
pubmed
[
ACTH and
cortisone in the treatment of
chronic lymphatic leukemia ] . -
pubmed
LIPASE FORMATION BY MICRO - ORGANISMS GROWN ON
HYDROCARBONS . -
pubmed
ON THE ROLE OF
FATTY ACID PEROXIDES IN ATHEROGENESIS . -
pubmed
Effect of
phosphodiesterase-5 inhibitor ,
sildenafil (
Viagra ) , in animal models of
airways disease . -
pubmed
Phosphodiesterase ( PDE )- 5 degrades
guanosine 3 ' , 5'cyclic monophosphate (
cGMP ) and its inhibitor
sildenafil citrate (
Viagra ) treats
erectile dysfunction by smooth muscle relaxation through elevated
cGMP . -
pubmed
Twenty - four hours after
ovalbumen exposure there was
airway hyperreactivity (
AHR ) to inhaled
histamine and significantly elevated macrophages , eosinophils , and
nitric oxide (
NO ) metabolites in bronchoalveolar lavage fluid . -
pubmed
Sildenafil treatment ( 1 mg / kg , intraperitoneally ) failed to affect the early and late responses but significantly reduced
AHR , leukocyte influx , and elevated
NO . -
pubmed
LPS exposure ( 30 microg / ml ) caused
AHR to
histamine at 1 hour and macrophage , eosinophil , and neutrophil influx at 24 hours with raised
NO . -
pubmed
Sildenafil pretreatment inhibited LPS - induced
AHR , leukocyte influx , and
NO generation . -
pubmed
The effectiveness of
sildenafil was not dependent on endogenous
NO because inhibition of
NO synthase with
Nomega - nitro - L - arginine methyl ester did not prevent its action . -
pubmed
Inhibition of
PDE5 by
sildenafil was confirmed by elevated
S - nitroso - N - acetylpenicillamine - induced
cGMP generation in isolated lungs . -
pubmed
A novel point mutation of the
RET protooncogene involving the second intracellular
tyrosine kinase domain in a family with
medullary thyroid carcinoma . -
pubmed
RET - activating mutations involve two important functional areas of the receptor : the cysteine - rich extracellular domain and the intracellular
tyrosine kinase domain . -
pubmed
Here , we describe a novel missense mutation of the
RET protooncogene that substitutes
arginine for
proline at codon 912 of the
intracellular tyrosine kinase domain in a family with
medullary thyroid carcinoma . -
pubmed
Morphological and cytological studies as well as determination of
ABA content and analysis of the expression mode of a
Lea gene , all point to its similarity to natural dormancy and germination of seeds . -
pubmed
Hexokinase inhibitors such as
glucosamine and
N - acetyl - glucosamine did not exert any influence on the regulation - deregulation effects of
sucrose . -
pubmed
It is thus inferred that this
sucrose - signaling pathway may be independent of
hexokinase . -
pubmed
This suggested the possibility that
cSUT has an important role in this
sucrose signal regulation system . -
pubmed
SST ,
wogonin-7 - O - glucuronoside ( a major
SST ingredient ) , and
wogonin ( an intestinal metabolite of
wogonin-7 - O - glucuronoside ) increased
CD4 /
CD8 ratio via a decrease of
CD8 + T - cell counts with no effect on
CD4 + T - cell counts . -
pubmed
Flow cytometric analyses of viability , proliferation , and cell cycle revealed that
wogonin suppressed
CD8 + T - cell proliferation without inducing cell
death . -
pubmed
These findings suggest that
SST may modulate the
CD4 /
CD8 ratio via the selective inhibition of
CD8 + T - cell proliferation by the
SST ingredient
wogonin-7 - O - glucuronoside or its metabolite
wogonin . -
pubmed
A canted antiferromagnetic structure is observed below
TN = 10 K , which remains stable down to 3 K , with an ordered magnetic moment of 2.44(7)mu(B ) for
Co2 + cations . -
pubmed
hominis cells were treated with
MAb 1D5 or control
MAb 5 , either with or without pretreatment with a pan - caspase inhibitor ,
zVAD.fmk , and/or a mitochondrial transition pore blocker ,
cyclosporine A ( CA ) . -
pubmed
Flow cytometric examination of cell size , mitochondrial membrane potential ( delta psi(m ) ) ,
caspase activation and in situ DNA fragmentation showed that
zVAD.fmk and CA , used independently or in combination , failed to inhibit
MAb 1D5 - mediated
PCD . -
pubmed
The sections observed under light microscope to evaluate the results of
hematoxylin and
eosin ( HE ) staining , Masson staining or
proliferative cell nuclear antigen(
PCNA ) and
alpha - smooth muscle actin (
alpha - SMA ) detected by immunohistochemistry . -
pubmed
It contained putative
copper - binding sites , a complement - like motif ( GCGWPRHM ) , a proteolytic activation site , and a conserved C - terminal region common to all known
proPOs . -
pubmed
The phage display technology offers an effective strategy for screening peptides which can interact with the
anti - Gal antibody to block
alpha - Gal antigen binding site . -
pubmed
Two peptide libraries , linear 7 peptide library and C7C library , were panned on the
anti - B monoclonal antibody which has the characteristic of binding to the
alpha - Gal antigenic epitope . -
pubmed
And new quantitative structure - activity relationships ( QSARs ) focused on the binding affinities of
aryl hydrocarbon receptor (
AhR ) ,
aryl hydrocarbon hydroxylase (
AHH ) and
7 - ethoxyresorufin O - deethylase (
EROD ) induction potencies of
PCDFs were developed . -
pubmed
9,10 - Bis ( 4 - hexyloxyphenyl )- 2,7 - diiodophenanthrene was selectively obtained by a one -
pot reaction of
10,10 - bis -( 4 - hexyloxy - phenyl )- 2,7 - diiodo-10H - phenanthren-9 - one with 1 equiv of
triethylsilane in
trifluoroacetic acid . -
pubmed
INTRODUCTION :
Cytochrome P450 1A2 (
CYP 1A2 ) is responsible for more than 90 % of
caffeine clearance . -
pubmed
Multivariate analysis revealed no evidence of any interaction effects of
caffeine with
CYP1A2 ( P=0.956 ) . -
pubmed
Differentiation conditions caused a three- to fourfold elevation in
cAMP level as well as
cAMP - dependent protein kinase activity . -
pubmed
Resistance against pro - oxidants from either stress or
cAMP may be associated with upregulation of the expression of three major antioxidant genes ,
peroxidoxin 1 ,
trypanothione reductase , and
superoxide dismutase A. -
pubmed
In contrast , downregulation of intracellular
cAMP by overexpression of
cAMP phosphodiesterase A resulted in a decrease in resistance against oxidative damage and reduced infectivity toward activated macrophages . -
pubmed
Superoxide dismutases (
SODs ) are a family of antioxidant enzymes that catalyse the degradation of toxic
superoxide radicals in obligate and facultative aerobic organisms . -
pubmed
All the genes identified (
sod1 to
sod17 ) have been cloned and sequenced , and shown to encode potentially functional dimeric
iron - containing
SOD isozymes . -
pubmed
cohnii cell lysate whereas
MnSOD and
Cu /
ZnSOD were not detected . -
pubmed
The nature of the resulting nanoparticles decorated multiwalled
carbon nanotubes are characterized by scanning electron microscopy ( SEM ) and transmission electron microscopic (
TEM ) analysis . -
pubmed
After debridement , the
wounds were covered with
rbFGF /
ACS and then bound up with sterile gauze in the
rbFGF /
ACS group ( n = 30 ) , or bound up with
petrolatum sterile gauze in the placebo group ( n = 28 ) . -
pubmed
On serological examination ,
GLU ,
PL , K and
ALT were increased significantly in 5 % females , and
GLU was increased significantly and
CRN was decreased significantly in the female 1.25 % group . -
pubmed
It was not significantly associated with blood pressure ,
hypertension , serum total or high density lipoprotein
cholesterol , body mass ,
diabetes ,
gout ,
osteoporosis ,
cardiovascular disease , smoking ,
caffeine use , or taking
calcium channel blockers or anticholesterol medications . -
pubmed
Some drugs ( antihistamines , antianxiety drugs , antidepressants , oral
steroids ) are associated with greater risk , while
angiotensin - converting enzyme inhibitors may be associated with lower risk . -
pubmed
Seminal analysis of the baseline prior to incubation of samples with different concentrations of
Alpha lipoic acids ( 0.00625 , 0.0125 , 0.025 , 0.05 , 0.1 mmol / ml ) was performed , and
post - seminal analysis was conducted after a one - hour incubation . -
pubmed
The cellular response to dsRNA or its synthetic analog
polyinosinic - polycytidylic acid ( poly I : C ) results in
IRF-3- ,
IRF-7- and
NF - kB - mediated activation of
type 1 IFNs and pro - inflammatory
cytokines critical for innate antiviral immune responses . -
pubmed
Ectopic expression and chronic activity of
FGFR1 in hepatocytes accelerates
diethylnitrosamine (
DEN)-initiated hepatocarcinogenesis by driving unrestrained cell proliferation and
tumor angiogenesis . -
pubmed
Hepatocyte
FGFR4 mediates liver 's role in systemic
cholesterol /
bile acid and lipid metabolism and affects proper hepatolobular restoration after damage without effect on cell proliferation . -
pubmed
Cast plates were fabricated from three types of gold alloys ( Type
IV , Type
II and low - gold ) and a
Co - Cr alloy . -
pubmed
In the specimens welded heterogeneously to
Co -
Cr , Type
IV was the greatest , followed by low - gold and Type
II . -
pubmed
There was no statistical difference ( P<0.05 ) in
fracture load between Type
II control and that welded to
Co -
Cr . -
pubmed
Higher elongations were obtained for Type
II in all conditions , whereas the lowest elongation occurred for low - gold welded to
Co -
Cr . -
pubmed
This study indicated that , of the three gold alloys tested , the Type
IV gold alloy was the most suitable alloy for laser - welding to
Co -
Cr . -
pubmed
Considering
liver tumor oncologic procedures , the aim of this study was to evaluate the accuracy of liver
iodine concentration ( IC ) estimated from
CCT images under such challenging conditions . -
pubmed
The accuracy of estimations using
CCT and computed tomographic scanners was evaluated ; an elliptical cylinder water phantom with
iodine solution inserts and seven patient data sets with transcatheter arterial chemoembolization were used . -
pubmed
The deduced
amino acid sequence showed to be identical with that of
Bm - M55 , and the recombinant protein was about Mr 55000 . -
pubmed
We also determined biological effects in
tumors treated with the antibody alone or in combination with
bevacizumab /
avastin ( an anti -
vascular endothelial growth factor antibody ) and/or
gemcitabine ( a chemotherapeutic agent ) . -
pubmed
When
HDGF - H3 was combined with either bevacizumab or
gemcitabine , we observed enhanced
tumor growth inhibition , particularly when the three agents were used together . -
pubmed
HDGF - H3 - treated
tumors exhibited significant reduction of microvessel density with a pattern distinctive from the microvessel reduction pattern observed in
bevacizumab - treated
tumors . -
pubmed
HDGF - H3 - treated but not
bevacizumab - treated
tumors also showed a significant increase of apoptosis . -
pubmed
RESULTS : At 2 years , systolic and diastolic blood pressure (
SBP /
DBP ) had decreased by 5.9/2.8 mm Hg ( 4.7/3.6 % ) in the added -
potassium - and -
calcium group and by 5.8/1.0 mm Hg ( 4.8/1.4 % ) in the salt - restricted group ; the values rose in the control group by 1.3/2.3 mm Hg ( 1.1/1.8 % ) . -
pubmed
Enhancement of chemical derivatization of
steroids by gas chromatography / mass spectrometry (
GC /
MS ) . -
pubmed
CONCLUSIONS : Most patients , who were regularly followed up in
CKD clinics , were acceptably adherent to the prescribed dietary protein intake restrictions ; the
LPD and
MPD did not lead to protein wasting ; and the
LPD did not decrease the risk of
death or dialysis therapy initiation compared with the
MPD . -
pubmed
This effect requires a
metabotropic glutamate receptor - mediated rise in
Ca ( 2 + ) in the astrocyte processes surrounding the neuron and the release of the gliotransmitter
ATP , which acts on postsynaptic
purinergic receptors . -
pubmed
All the treatments significantly reduced
lactate dehydrogenase LDH efflux after reoxygenation ( -54.1 % for
aspirin , -51.3 % for VOO and -72.9 % for
aspirin plus VOO ) . -
pubmed
The activity of the inducible isoform (
iNOS ) was inhibited by the three types of treatment ( -31.8 % for
ASA , -29.1 % for VOO and -56.0 % for
ASA plus VOO ) . -
pubmed
The antibody ,
PIPA , protecting against small lethal challenges only is in the fraction A(2 ) containing mainly
11 S globulin . -
pubmed
The PTs were incubated in a 50 % mixture of Ham 's
F12 : Dulbecco 's medium containing only
penicillin and dextran as supplements . -
pubmed
CHRNB4 , which encodes the
nAChR beta4 subunit , plays a major role in the molecular mechanisms that govern
nicotine withdrawal . -
pubmed
In addition , dietary
astaxanthin increased concentrations of
IgG and
IgM , and B cell population . -
pubmed
AIM : Oxidative stress is caused by imbalance between the productions of reactive
oxygen species ( ROS ) and antioxidant defense mechanisms . -
pubmed
Palm oil antioxidants such as
tocotrienol rich fraction (
TRF ) is known to have neuroprotective effects on neurones by acting against free radical induced neuronal cell
death . -
pubmed
PURPOSE : The use of the high - dose
corticotrophin stimulation test ( HDCST ) as a guide to use low - dose
steroid therapy in
septic shock is controversial . -
pubmed
The
adrenocotropin hormone (
ACTH ) constitutes the immediate stimuli to produce
cortisol . -
pubmed
However , we are not aware of any study investigating the effects of
minocycline on
caspase - independent programmed cell
death (
PCD ) pathways . -
pubmed
Minocycline exhibited neuroprotection and inhibition of the toxin - induced
caspase-3 - like activity , DNA fragmentation , and chromatin condensation , hallmarks of apoptosis . -
pubmed
Moreover , we revealed that
6 - OHDA also activated
caspase - independent PCDs ( such as paraptosis ) , which required de novo protein synthesis . -
pubmed
Additionally , by separately monitoring
caspase - dependent and
caspase - independent pathways , we showed that inhibition of apoptosis only partially explained the protective effect of
minocycline . -
pubmed
In conclusion , we report for the first time evidence indicating that
minocycline may inhibit
PCD pathways that are additional to conventional apoptosis . -
pubmed
CONCLUSIONS : Our results show that
claudin-6 protein is significantly down - regulated in
breast invasive ductal carcinomas and is an important correlate with
lymphatic metastasis , but
claudin-6 down - regulation was not correlated with upregulation of the methylation associated proteins (
MeCP2 ,
DNMT1 ) or
histone modification associated proteins (
HDAC1 ,
H3Ac , H4Ac ) . -
pubmed
Treatment with
vildagliptin showed increase in the number of
insulin - positive beta - cells in islets and improved the expressions of genes and proteins are responsible for
insulin secretions . -
pubmed
Direct treatment with the three
nitroderivatives decreased ROS generation compared to the control and
NO - HTy at 20muM also increased
glutathione peroxidase (
GPx ) activity ( p<0.001 ) . -
pubmed
Pretreatment with the three
nitroderivatives at 5 - 20 muM counteracted
t - BOOH cell damage by decreasing ROS generation ( p<0.001 ) and
malondialdehyde (
MDA ) levels ( p<0.001 ) , increasing
reduced glutathione ( p<0.001 ) and disminishing
GPx ( p<0.05 ) activity . -
pubmed
NO - HTy ,
NO - HTy - A and
NO - HTy - E decreased
glutathione reductase activity ( p<0.05 ) . -
pubmed
Our laboratory has previously demonstrated that reactive
oxygen species , mediated by
p22 ( phox )- based Nox oxidases , are enhanced in
VHL - deficient cells and have a role in the activation of
Akt on S473 , a site phosphorylated by the
mTORC2 complex . -
pubmed
Channel - mediated astrocytic
glutamate release via
Bestrophin-1 targets synaptic NMDARs . -
pubmed
Our recent study demonstrated that astrocytes release
glutamate upon
GPCR activation via
Ca2 + activated anion channel ,
Bestrophin-1 (
Best1 ) . -
pubmed
The target of
Best1 - mediated astrocytic
glutamate has been shown to be the neuronal
NMDA receptors (
NMDAR ) . -
pubmed
FINDINGS : We recorded spontaneous miniature excitatory postsynaptic currents ( mEPSCs ) from
CA1 pyramidal cells to test whether
Best1 - mediated astrocytic
glutamate targets synaptic NMDAR . -
pubmed
An agonist of
protease activated receptor 1 (
PAR1 ) was used to induce astrocytic
Ca ( 2 + ) increase and
glutamate release . -
pubmed
Firstly , we found that activation of
PAR1 and subsequent release of
glutamate from astrocyte does not alone increase the frequency of mEPSCs . -
pubmed
Astrocytic
Best1 - mediated
glutamate release by
PAR1 activation did not affect decay kinetics , frequency , and amplitude of fast rising mEPSC . -
pubmed
CONCLUSIONS : Our results indicate that astrocytic
glutamate via
Best1 channel targets and activates synaptic NMDARs . -
pubmed
As a consequence , the serum
cortisol half - life ( T1/2 ) is prolonged ,
ACTH is suppressed , and serum
cortisol concentration is normal . -
pubmed
We report three siblings - two female and one male - with the
syndrome of apparent mineralocorticoid excess who presented with
hypertension ,
hypokalemia , low
renin , and low
aldosterone levels . -
pubmed
The addition of
spironolactone resulted in a
decrease in blood pressure , rise in serum
potassium and a gradual increase in
plasma renin activity in all three . -
pubmed
Urinary
steroid profile and molecular analysis confirmed the diagnosis of non - classic
congenital adrenal hyperplasia (
NCCAH ) due to
21 - hydroxylase deficiency . -
pubmed
Pretreated sample on enzymatic hydrolysis at 50 C for 20 h with cocktail of
cellulase ,
xylanase and
laccase produced by locally isolated consortia released 668.9 mg / g of total
sugar and 265.06 mg / g of total
polyphenols . -
pubmed
Rab proteins are a large family of
GTP - binding proteins that regulate cellular membrane traffic and organelle identity . -
pubmed
Dephosphorylation of the
E2P phosphoenzyme intermediate of the
sarcoplasmic reticulum Ca ( 2 +)- ATPase was studied using density functional theory . -
pubmed
The optimized structure of the transition state reveals considerable bond breakage between
phosphorus and the
Asp351 oxygen ( distance
2.19 A ) and little bond formation to the attacking water
oxygen ( distance 2.26 A ) . -
pubmed
These actions of
clenbuterol were prevented by pre - treatment with the non - selective
beta - adrenoceptor antagonist
propranolol , the
beta2 - adrenoceptor antagonist
ICI-118,551 , but not the
beta1 - adrenoceptor antagonist
metoprolol , suggesting that the effects of
clenbuterol on
IkappaBalpha expression and
NFkappaB activity are mediated specifically by the
beta2 - adrenoceptor . -
pubmed
In addition , the actions of
clenbuterol were mimicked by systemic administration of another highly selective long - acting
beta2 - adrenoceptor agonist
formoterol . -
pubmed
As
neurodegenerative diseases are associated with
inflammation we determined if
clenbuterol could suppress
NFkappaB activation that occurs in response to an inflammatory stimulus . -
pubmed
In tandem ,
clenbuterol blocked expression of the
NFkappaB - inducible genes
TNF - alpha and
ICAM-1 following LPS administration . -
pubmed
Our finding that
clenbuterol and
formoterol inhibit
NFkappaB activity in the CNS further supports the idea that
beta2 - adrenoceptors may be an attractive target for treating neuroinflammation and combating
inflammation - related neurodegeneration . -
pubmed
These interactions are absent in a mutant
strain with genomically encoded
RNase E that lacks the C - terminal half , supporting the role of the
carboxy - end domain as the scaffold for the degradosome . -
pubmed
The data support a model for the RNA degradosome (
RNAD ) , in which the
RNase E carboxy - end is proximal to
PNPase , more distant to
Enolase , and more than 10 nm from
RhlB helicase . -
pubmed
Replacement of the catalytic nucleophile
aspartyl residue of
dextran glucosidase by
cysteine sulfinate enhances transglycosylation activity . -
pubmed
This
enzyme has an
Asp-194 catalytic nucleophile and two catalytically unrelated
Cys residues ,
Cys-129 and
Cys-532 . -
pubmed
The nucleophile mutant of
2CS was generated by substitution of
Asp-194 with
Cys ( D194C-2CS ) . -
pubmed
As a member of imitation switch (
ISWI ) family in
ATP - dependent chromatin remodeling factors ,
RSF complex consists of
SNF2h ATPase and
Rsf-1 . -
pubmed
A snapshot of
ubiquitin chain elongation :
lysine 48 - tetra -
ubiquitin slows down ubiquitination . -
pubmed
Using " elongation intermediates " containing
beta - catenin linked with Ub chains of defined length , it was observed that a
Lys-48 - Ub chain of a length greater than four , but not its
Lys-63 linkage counterparts , slowed the rate of additional Ub conjugation . -
pubmed
Given that
Lys-48 - tetra - Ub is packed into compact conformations due to extensive intrachain interactions between Ub subunits , this topology may limit the accessibility of
SCF(
betaTrCP)/
Cdc34 to the distal Ub
Lys-48 and result in slowed elongation . -
pubmed
Glucose is transported across the cell membrane by specific saturable transport system , which includes two types of
glucose transporters : 1 )
sodium dependent glucose transporters (
SGLTs ) which transport
glucose against its concentration gradient and 2 )
sodium independent
glucose transporters (
GLUTs ) , which transport
glucose by facilitative diffusion in its concentration gradient . -
pubmed
The more glycosylated
GLUT1 is produced in brain microvasculature and ensures
glucose transport across the blood brain barrier ( BBB ) . -
pubmed
Glucose transported to astrocytes by
GLUT1 is metabolized to
lactate serving to neurons as energy source . -
pubmed
GLUT2 is present in hypothalamic neurons and serves as a
glucose sensor in regulation of food intake . -
pubmed
GLUT3 is the most abundant
glucose transporter in the brain having five times higher transport capacity than
GLUT1 . -
pubmed
GLUT5 is predominantly
fructose transporter . -
pubmed
In brain ,
GLUT5 is the only
hexose transporter in microglia , whose regulation is not yet clear . -
pubmed
GLUT4 and
GLUT8 are
insulin - regulated
glucose transporters in neuronal cell bodies in the cortex and cerebellum , but mainly in the hippocampus and amygdala , where they maintain hippocampus - dependent cognitive functions . -
pubmed
Insulin translocates
GLUT4 from cytosol to plasma membrane to transport
glucose into cells , and
GLUT8 from cytosol to rough endoplasmic reticulum to recover redundant
glucose to cytosol after protein glycosylation . -
pubmed
For both
SHP and
CHP , the magnitude of hydraulic pressure that induced the greatest release of
ATP during FSS was 15 mmHg . -
pubmed
Decreasing the magnitude of pressure during FSS to atmospheric pressure reduced
ATP release to that of basal
ATP release from static cells and inhibited
actin reorganization into stress fibers that normally occurred during FSS with 15 mmHg of pressure . -
pubmed
Our study found that
acetylcholine (
ACh ) was a key transmitter that derepressed the phenotype induced by
LH . -
pubmed
A phase II study of
erlotinib monotherapy in pre - treated
non - small cell lung cancer without
EGFR gene mutation who have never / light smoking history : re - evaluation of
EGFR gene status ( NEJ006 / TCOG0903 ) . -
pubmed
CONCLUSIONS : Re - examination of
EGFR gene status using different detecting method or different sample should be considered to grasp a chance of
erlotinib treatment after first line treatment . -
pubmed
In confirmed
EGFR wild
NSCLC , negative
HGF staining could be a biomarker for longer PFS by
erlotonib treatment . -
pubmed
AIM : To evaluate in vitro
cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active
calcium silicate systems (
CS ) and
hydroxyapatite ( HA -
CS ) . -
pubmed
After 24 h elution , cell viability was 10 + - 1.8 % , 49.5 + - 4.2 % and 61 + - 7.4 % , for
MTA , and HA -
CS , respectively . -
pubmed
However ,
CS and HA -
CS were significantly less toxic than the control material
MTA ( P & lt ; 0.05 ) . -
pubmed
Histopathological examination indicated similar inflammatory reaction ,
vascular congestion and connective tissue integrity associated with
CS , HA -
CS and
MTA at each observation period ( P & gt ; 0.05 ) . -
pubmed
The only significant difference was found for capsule thickness , that is thicker capsule was associated with HA -
CS compared to
MTA at 60 days ( P = 0.0039 ) . -
pubmed
HA -
CS induced moderately thick capsules ( median score 3 , score range 2 - 3 ) , whereas
MTA resulted in thin capsule formation ( median score 2 , score range 1 - 3 ) . -
pubmed
This treatment did not modify the
SERCA1a affinity for
Ca ( 2 + ) but increased the
Ca ( 2 +)-dependent
ATPase activity of SR membranes indicating that the S - acylation of
SLN or of other proteins is required for this effect on
SERCA1a . -
pubmed
An alignment of 67
SLN sequences from the protein databases shows that 19 of them contain a
cysteine and the
rest a
phenylalanine at position 9 . -
pubmed
SITA severely impaired serum
DPP-4 activity , but had no effect on glycaemia . -
pubmed
In addition ,
SITA reduced resting tension of isolated single cardiomyocytes and intensified phosphorylation of the sarcomeric protein
titin . -
pubmed
SITA also increased LV concentrations of
cGMP and increased activity of
protein kinase G (
PKG ) . -
pubmed
These effects seem at least partially mediated by stimulatory effects on the myocardial
cGMP -
PKG pathway and , hence , on the phosphorylation status of
titin and the hereto coupled cardiomyocyte stiffness modulus . -
pubmed
A sensitive strategy for the fluorescence detection of
DNA methyltransferase activity based on the
graphene oxide platform and
T7 exonuclease - assisted cyclic signal amplification . -
pubmed
In this work , a simple fluorescence strategy based on the
graphene oxide ( GO ) platform and
T7 exonuclease ( T7 Exo)-assisted cyclic signal amplification is developed for the fast and sensitive detection of
DNA methyltransferase (
MTase ) activity and inhibition . -
pubmed
The released ssDNA subsequently hybridized with a
FAM - labeled signal probe (
DP ) to form a duplex with a blunt 5'-terminal of DP and a 4 - mer overhang at the 5'-end of the released ssDNA . -
pubmed
Aquaporins Contribute to
ABA - Triggered Stomatal Closure through
OST1 - Mediated Phosphorylation . -
pubmed
ABA induced a 2 - fold increase in osmotic water permeability ( Pf ) of guard cell protoplasts and an accumulation of reactive
oxygen species in guard cells , which were both abrogated in
pip2;1 plants . -
pubmed
Open stomata 1 (
OST1)/
Snf1 - related
protein kinase 2.6 (
SnRK2.6 ) , a
protein kinase involved in guard cell
ABA signaling , was able to phosphorylate a cytosolic PIP2;1 peptide at
Ser-121 . -
pubmed
Upon expression in
pip2;1 plants , a phosphomimetic form ( Ser121Asp ) but not a phosphodeficient form ( Ser121Ala ) of
PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its
ABA - dependent activation and was able to restore
ABA - dependent stomatal closure in
pip2;1 . -
pubmed
This work supports a model whereby
ABA - triggered stomatal closure requires an increase in guard cell permeability to water and possibly
hydrogen peroxide , through
OST1 - dependent phosphorylation of PIP2;1 at
Ser-121 . -
pubmed
Insulin secretion and sensitivity were measured by the gold - standard Botnia clamp , which is a combination of a 1 - h intravenous
glucose tolerance test ( IVGTT ) followed by 3 - h hyperinsulinemic euglycemic
clamp . -
pubmed
Fasting
glucose and
insulin were 7.3 + - 2.4 mmol / L and 10.6 + -
6.3 mU / L , respectively . -
pubmed
Two epimeric metabolites of
Larotaxel were synthesized in eight steps from
10 - DAB III as a commercial material and their structures were characterized using NMR and
MS spectral data . -
pubmed
Acetolactate synthase is a mitochondrial enzyme that catalyses the conversion of two
pyruvate molecules to an
acetolactate molecule with release of
carbon dioxide . -
pubmed
RESULTS : In Germany , mean levels of C16:1n-7 t , a marker for dairy and meat intake , decreased , as did mean levels of industrially produced ( IP)-
TFA , as did the percentage of individuals with
IP -
TFA & gt ; 1.04 % . -
pubmed
The HR for all - cause mortality of CACS & gt;=400 , in those with lower exercise capacity , is estimated to be about three times of that in those with higher exercise capacity ( HR 3.328 in & lt;10 METs vs. 1.108 in & gt;=10 METs , p for interaction = 0.024 ) after adjustment for age , gender , fasting
glucose ,
creatinine ,
alanine transaminase and
albumin . -
pubmed
The symmetry of the room - temperature ( RT ) structure of title compounds
La2 - xSrxCoTiO6 - delta changes with x , from
P21 / n ( 0 & lt;= x & lt;= 0.2 ) to Pnma ( 0.3 & lt;= x & lt;= 0.5 ) and to R3c ( 0.6 & lt;= x & lt;= 1 ) . -
pubmed
Therefore , this review focuses on the role that the interaction between
vitamin D ,
VDR function , and gut microbiome might have on
autoimmune diseases in the context of the hygiene hypothesis . -
pubmed
The biologically active form of
vitamin D ,
1,25 ( OH ) 2D3 , serves as the primary ligand for
VDRs , which have been shown to play a fundamental role in reducing
autoimmune disease symptoms . -
pubmed
Remarkably , evidence shows that hormonal compounds and byproducts of the microbiota such as secondary
bile acids might also activate
VDR . -
pubmed
We have gained insights on how the
VDR functions affects
inflammation , autophagy , and microbiota composition that could lead to the development of pathogenesis of
autoimmune diseases , while confirming the role
vitamin D and
VDRs have in the context of hygiene hypothesis . -
pubmed
Although they have similar catalytic
triads and
oxyanion holes ,
esterases are
hydrolases and
homoserine transacetylases (
HTAs ) are
transferases . -
pubmed
Est22 could efficiently hydrolyze
esters with
acyl lengths of up to six
carbon atoms but had no
transacetylase activity , which is different from
HTAs and
HTA - like
acetyl esterases . -
pubmed
We solved the structures of
Est22 and its L374D mutant and modeled the structure of the L374D mutant with
p - nitrophenyl butyrate . -
pubmed
Esterases catalyze the hydrolysis of simple
esters and are widely used in the pharmaceutical and agrochemical industries , while
HTAs catalyze the transfer of an
acetyl group from
acetyl - coenzyme A (
CoA ) to
homoserine and are essential for microbial growth . -
pubmed
The atypical
vesicular glutamate transporter type 3 (
VGLUT3 ) is expressed by subpopulations of neurons using
acetylcholine ,
GABA , or
serotonin as neurotransmitters . -
pubmed
SIGNIFICANCE STATEMENT
VGLUT3 is an atypical member of the vesicular
glutamate transporter family . -
pubmed
Overall , BA contents decreased with
NaCl level ( 2785.1mgkg-1 , 1148.1mgkg-1 and 307.7mgkg-1 ) and increased with ageing time ( 366.2mgkg-1 , 1711.8mgkg-1 and 2959.2mgkg-1 in the final product ( T0 ) , and after 1 (
T1 ) and 3 ( T3 ) months of ageing , respectively ) . -
pubmed
The expression levels of
osteoblast growth and differentiation factors (
runt - related transcription factor 2 ,
osteogenic protein-1 , and
alkaline phosphatase ) , apoptosis - related genes (
transformation - related protein 53 ,
ataxia telangiectasia mutated protein ,
caspase-3 , and
caspase-8 ) , and
Akt1 and
phospho -
Akt (
Thr308 ) were then assessed via
alkaline phosphatase staining ,
acridine orange -
propidium iodide staining ,
annexin V /
PI apoptosis assay , real - time RT - PCR , and Western blot analyses . -
pubmed
Moreover , glucocorticoid treatment yielded increased
transformation - related protein 53 ,
ataxia telangiectasia mutated protein ,
caspase-3 , and
caspase-8 expression and decreased
Akt1 and
phospho -
Akt levels , indicating glucocorticoid - induced apoptosis . -
pubmed
Meanwhile ,
beta - ecdysone inhibited glucocorticoid function , preserving the expression of
Akt1 and
phospho -
Akt and reducing the expression of
transformation - related protein 53 ,
ataxia telangiectasia mutated protein ,
caspase-3 , and
caspase-8 . -
pubmed
Proprotein Convertase Subtilisin / Kexin Type 9 Inhibitor Therapy - Breakthrough in Low - Density Lipoprotein
Cholesterol Lowering , Breakdown in Value . -
pubmed
Here , we demonstrate the stability and dynamics of a tile - based 6 - helix DNA nanotube (
DNT ) embedded in
POPC lipid bilayer using the analysis of 0.2 mus long equilibrium MD simulation trajectories . -
pubmed
We observe that the head groups of the lipid molecules close to the lumen cooperatively tilt towards the hydrophilic
sugar -
phosphate backbone of DNA and form a toroidal structure around the patch of
DNT protruding in the membrane . -
pubmed
In this study , the effect on pore volume and compressive strength was investigated using nano - sized blast furnace slag ( BFS ) , fly ash (
FA ) , and desulfurized
gypsum ( DG ) . -
pubmed
In the chemical compositions of BFS and
FA , the sum of the four ingredients ,
CaO ,
SiO2 ,
A12O3 , and
MgO were shown to account for 97 % and 87 % , respectively . -
pubmed
The mean
CA-125 level was 80.04 U / mL at baseline and significantly decreased to 33.11 U / mL after taking
DNG for 24 weeks and lasted until 72 weeks ( P for trend = .0288 ) . -
pubmed
Then the
Congo red (
CR ) removal efficiency by DAB was optimized based on the flocculation conditions , including wastewater initial pH , flocculant dosage and initial dye concentration . -
pubmed
A Phase
IV Efficacy Study of
Formeta Plus
Carboplatin as First - Line Treatment of Advanced Non - Squamous ,
Non - SmallCell Lung Cancer in Iran : An Affordable Price with Clinical Benefit Background : This study performed to assess the efficacy and safety of
Formeta ( generic form of
Pemetrexed ) plusCarboplatin as first - line chemotherapy in advanced stage ,
non - squamous , non small cell lung cancer (
NSCLC ) in Iran . -
pubmed
Methods : This was a
post marketing single - arm phase
IV efficacy study of
Formeta ( manufactured by Oncomed . -
pubmed
R406 elicits anti - Warburg effect via
Syk - dependent and -independent mechanisms to trigger apoptosis in
glioma stem cells . -
pubmed
Utilizing an anti -
cancer compound library , we identified
R406 , the active metabolite of a FDA - approved
Syk inhibitor for
immune thrombocytopenia (
ITP ) , with remarkable
cytotoxicity against GSCs but not normal neural stem cells . -
pubmed
Mechanistically , the anti - GSC effect of
R406 was due to the disruption of
Syk /
PI3 K signaling in
Syk - positive GSCs and
PI3K /
Akt pathway in
Syk - negative GSCs respectively . -
pubmed
Overall , these findings not only identify
R406 as a promising
GSC - targeting agent but also reveal the important role of
Syk and
PI3 K pathways in the regulation of energy metabolism in GSCs . -
pubmed
RESULTS :
Post - warming
trypan blue staining showed the survival of most cells , although membrane integrity immediately after warming ( 66.00 % + - 4.73 ) or after 2 h IVC ( 59.67 % + - 4.18 ) was significantly lower than controls ( C0h 89.67 % + - 1.45 ) . -
pubmed
Synthesis , Structural Studies , and
alpha - Glucosidase Inhibitory , Antidiabetic , and Antioxidant Activities of
2,3 - Dihydroquinazolin-4 ( 1H )- ones Derived from
Pyrazol-4 - carbaldehyde and
Anilines . -
pubmed
A series of new
quinazoline derivatives were designed and synthesized via a one -
pot condensation reaction between
isatoic anhydride and
aromatic aldehydes with
anilines using
aluminum sulfate as a catalyst in refluxing
ethanol . -
pubmed
The entire synthesized compounds showed the potent
alpha - glucosidase inhibitory activity compared with
acarbose as a standard material . -
pubmed
Amongst , the compounds 4h and 4i showed the strongest
enzyme inhibitory potentials than the standard drug
acarbose . -
pubmed
AIM : Aims were to test the effectiveness of an organizational change intervention integrating smoking cessation treatment into usual
alcohol and other drug ( AOD ) treatment , compared with usual care , on ( 1 ) 7 - day point prevalence abstinence ( PPA ) at 8 weeks follow - up ; ( 2 ) prolonged abstinence ; ( 3 ) cigarettes smoked per day ; ( 4 ) number of quit attempts ; and ( 5 ) offer and use of
nicotine replacement therapy ( NRT ) . -
pubmed
The spinal LTP was remarkably reversed by the spinal application of either BLA or
gastrin - releasing peptide receptor (
GRPR ) antagonist (
PD176252 ) . -
pubmed
Together ,
BLA was effective in inhibiting
histamine - dependent and
histamine - independent
itches , and the mechanisms underlying these effects were involved but not limited to the inhibition of
GRP -
GRPR signaling in the spinal dorsal horn . -
pubmed
These were :
triazoles for inhibition of
retinoic acid ( RA ) metabolism ,
AM580 and
CD3254 for selective activation of respectively
RA - receptor (
RAR ) and
retinoid - X - receptor (
RXR ) ,
dithiocarbamates for inhibition of
lysyl oxidase ,
TCDD for activation of the
aryl - hydrocarbon - receptor (
AhR ) ,
VPA for inhibition of
histone deacetylase (
HDAC ) , and
PFOS for activation of
peroxisome proliferator - activated receptor - alpha (
PPARalpha ) . -
pubmed
In this way ,
2,4 - dinitrophenol matched with the
TCDD and
RAR profiles ,
boric acid with
RAR ,
endosulfan with
PFOS ,
fenpropimorph with
dithiocarbamates ,
PCB126 with
AhR , and RA with
triazoles and
RAR profiles . -
pubmed
In the absence of a cocatalyst , the as - obtained colloidal
CdS -
NC photocatalyst achieves a superb
H2 - evolution rate of 6.86 mmol h-1 g-1 and an apparent quantum efficiency of 52.8 % under visible light . -
pubmed
One -
pot access to
2 - amino-3 - arylbenzofurans : direct entry to polyheterocyclic chemical space . -
pubmed
As a means to make new
benzofuran - embedded polycyclic structures , we established two efficient one -
pot sequential coupling routes to
2 - amino-3 - arylbenzofurans and
2 - amino-3 - arylnaphtho [ 2,1 - b ] furans . -
pubmed
Further ring formation ( six- and seven - membered rings ) with the resulting
amine moiety at the
C2 position of
benzofurans was realized , leading to further expansion of
benzofuran - based chemical space . -
pubmed
It also allowed us to uncover the functions of hypothetical proteins , such as
Sll0445 ,
Sll0446 , and
Sll0447 , participating in photosynthesis and cell motility , and
Sll1334 in regulating
fatty acid biogenesis . -
pubmed
Stimulation of cerebral
histamine H2 receptors by
clonidine . -
pubmed
In order to determine the influence of
copper additives on the protein digestibility , the complex formation of
proteins with
Cu ions under acidic conditions ( pH=2.2 ) was investigated . -
pubmed
pepsin was mixed with 10 mumol ( 2.49 mg )
CuSO4 - 5H2O and dialysed against low molecular complexing agents with increasing affinity towards
copper ions . -
pubmed
The remaining
copper ions in
pepsin could only be removed by
alanin and
ammonium - tetramethylendithiocarbamate . -
pubmed
Maximum incorporation of
3H-5 - HTP is achieved by 2 h and that of
3H-5 - HT by 20 minutes
post injection . -
pubmed
Results indicated that chewable
isosorbide dinitrate reduced
systolic blood pressure and the triple product (
systolic blood pressure X heart rate X ejection time ) significantly during
rest and reduced the left ventricular ejection time corrected for heart rate both at
rest and peak exercise ; no significant differences were observed in the placebo group . -
pubmed
Complex I and
IV subunits were more affected than complex V subunits , and there was a rough correlation with both protein size and number of
lysine residues . -
pubmed
BW ) resulted in a marked and sustained elevation of
serum growth hormone (
GH ) and a slight and delayed increase in plasma
prostaglandin E2 - M. -
pubmed
delta psi dissipation requires
SecA ,
ATP , and
proOmpA. -
pubmed
proOmpA which , due to the covalent addition of a folded protein to a
cysteinyl side chain , is arrested during its translocation , can nevertheless cause the loss of delta psi . -
pubmed
This translocation - specific reduction in delta psi is only seen in the presence of
halide anions , although
halide anions are not needed for
proOmpA translocation per se . -
pubmed
Loss of lymphoproliferative responses to
antigen occurs early in infected children and precedes the loss of
CD4 + helper cells and of
PHA responsiveness . -
pubmed
Effect of exercise training on
insulin sensitivity and
glucose metabolism in lean ,
obese , and
diabetic men . -
pubmed
Before and 4 - 5 days after the last training session , euglycemic hyperinsulinemic ( 40 mU.m2.min-1 )
clamps were performed at a plasma
glucose of 90 mg / dl , combined with indirect calorimetry . -
pubmed
Total
insulin - stimulated
glucose disposal ( M ) was corrected for residual hepatic
glucose output . -
pubmed
Insulin sensitivity was not significantly altered by training in any group , but basal hepatic
glucose production was reduced by 22 % in the
diabetic men . -
pubmed
Superfusion of NILs with the beta - adrenergic agonist
ISO ( 30 min
pulse ) resulted in a rapid , sustained and concentration - dependent stimulation of the release of beta E - like immunoreactivity (
beta E - IR ) over basal as determined with an antiserum directed against the C - terminus of the beta E- ( 1 - 31 ) sequence ( 10(-6 ) M : + 145 % ; 10(-7 ) M : + 73 % ; 10(-8 ) m : + 41 % ) . -
pubmed
These effects of
ISO were antagonized by the competitive
alpha - adrenoceptor antagonist
propranolol in a concentration - dependent manner , indicating the involvement of
alpha - adrenoceptors . -
pubmed
The beta - related peptides released from the NILs under basal and
ISO - stimulated conditions were further characterized , based on their retention times in a reversed - phase HPLC system and their reactivity with specific antisera recognizing respectively the midportion of
beta E , the N - terminus of
acetylated endorphins , the C - terminus of
tau - endorphin (
beta E -( 1 - 17 ) ;
tau E ) , or the C - terminus of
alpha - endorphin ( beta E-(1 - 16 ) ;
alpha E ) . -
pubmed
Six smaller peaks were observed , with the characteristics of beta E-(1 - 26 ) and beta E-(1 - 27 ) ( these peptides were not resolved with the HPLC system used ) , Ac tau E ,
tau E , Aa alpha E , and
des - tyrosine - alpha E ( DT alpha E ) , respectively . -
pubmed
The
IgG monoclonals
LRB 21 and
LRB 45 recognized a spatially close together or identical
apoC - I epitope . -
pubmed
Fertility
alpha 2 - microglobulin reacts with 3 out of 8
lectins , which possess affinity to
monosaccharides (
glucose and
mannose ) and
acetylamino sugar . -
pubmed
The results indicate that the
7F7 antigen and the
P3.58 melanoma antigen are identical , that some
epitopes of this
adherence molecule are differentially expressed on individual cell types and that one epitope , which is only weakly expressed on activated T cells , does not contribute to the role of the
7F7 antigen in some examples of cell - cell adherence . -
pubmed
This effect was mimicked by the M2 ligand
AF - DX 116 , but neither the
M1 - selective antagonist
pirenzepine , nor the putative M3 - muscarinic antagonist ,
4 - diphenylacetoxy - N - methylpiperidine (
4 - DAMP ) , altered
ACh release . -
pubmed
The
beta - carbolines , which are potent ligands for
benzodiazepine receptors , antagonize the pharmacological actions of
benzodiazepines . -
pubmed
These findings indicate that the
beta - carboline functionally interacts with
benzodiazepine receptors in a manner unlike that seen with typical agonists and antagonists . -
pubmed
Further evidence of
phencyclidine /
sigma opioid receptor commonality . -
pubmed
Testosterone is able to restore
prolactin binding in hypophysectomized animals , as
PRL treatment does . -
pubmed
Uptake of the following precursors was investigated :
apocytochrome c ,
ADP /
ATP carrier and subunit 9 of the
oligomycin - sensitive
ATPase . -
pubmed
Concentrations of added
apocytochrome c exceeding the concentrations of precursor proteins synthesized in vitro by a factor of about 10(4 ) did not inhibit the transfer of
ADP /
ATP carrier or
ATPase subunit 9 into mitochondria . -
pubmed
Carbonylcyanide m - chlorophenylhydrazone , an uncoupler of oxidate phosphorylation , inhibited transfer in vitro of
ADP /
ATP carrier and of
ATPase subunit 9 , but not of
cytochrome c. -
pubmed
Both follicular fluid and granulosa cell homogenate , in vitro , catabolized
125I - labelled
hCG to hormone sub - units . -
pubmed
The many
faces of
methylmercury poisoning . -
pubmed
Modification of
enoyl - CoA hydratase using
diethyl pyrocarbonate . -
pubmed
Diethyl pyrocarbonate inactivates
enoyl - CoA hydratase (
L-3 - hydroxyacyl - CoA hydro - lyase ,
EC 4.2.1.17 ) with a second - order rate constant of 1.3 M-1 X s-1 . -
pubmed
The single
histidine per
enzyme subunit is completely modified at a rate considerably faster than inactivation , and enzymatic activity is not restored by treatment with
hydroxylamine . -
pubmed
Destruction of N - terminal
serine residue by
periodate oxidation lowers , but does not abolish
enzyme activity . -
pubmed
Modification with
diethyl pyrocarbonate does not lead to any gross changes in the structure of the
enzyme . -
pubmed
These experiments taken together show that , in contrast to other
hydratases ,
histidine is not involved in the catalytic mechanism of
enoyl - CoA hydratase , and suggest that a single residue is important for activity . -
pubmed
The primary evidence for this conclusion is that ventral tegmental electrolytic or
6 - OHDA lesions which damaged specifically these fibers invariably caused : ( 1 ) a marked reduction of CA varicosities in the perifornical area ; ( 2 ) a strong reduction or loss of the anorectic response produced by perifornical injection of the presynaptically acting drugs
amphetamine and
mazindol ; and ( 3 ) a potentiation of the anorectic response produced by perifornical injection of the
CA receptor agonists
dopamine and
epinephrine . -
pubmed
On subjecting 2162 - fold purified
enzyme preparations to
polyacrylamide - gel electrophoresis , a single band of protein coincident with the zone of enzyme activity and having an apparent molecular weight of 205 000 + /- 10 000 was identified . -
pubmed
Some metalloenzyme properties of the
phosphatase were also studied and it was demonstrated that both
Mg2 + and
Zn2 + ions are necessary for hydrolytic activity . -
pubmed
The
sialic acid residues , however , do not appear to constitute part of the active centre of the
enzyme . -
pubmed
The
enzyme also hydrolysed a variety of compounds having either
phosphomonoester or
pyrophosphate linkages and was inactivated after heating at 60 degrees C for 15 min . -
pubmed
Histone , protamine and
myelin basic protein significantly stimulated
cyclic GMP formation , both in a time- and concentration - dependent manner . -
pubmed
Removal of the extracellular / intracellular
Ca2 + gradient by a
Ca2 + chelator completely abolished the
cyclic GMP responses elicited by
histone and protamine , suggesting that influx of extracellular
Ca2 + might be involved in their activation of
NO synthase . -
pubmed
The effects of
myelin basic protein on
cyclic GMP formation , however , appeared to be due to
Ca2 + release from intracellular stores . -
pubmed
Generation of active
oxygen forms ( GAOF ) by alveolar macrophages (
AM ) and leukocytes , the role of
GAOF in provoking a
bronchial asthma ( BA ) attack were studied simultaneously and compared in 10
asthmatics , 10 chronics with
bronchitis and 5 healthy subjects . -
pubmed
GAOF was evaluated using
luminol - dependent chemiluminescence . -
pubmed
It appeared that in BA aggravation leukocytes generate more active
oxygen forms , but
AM activation surpasses that of leucocytes . -
pubmed
This study noninvasively measured
hemoglobin oxygen saturation in young ( n = 6 , 28 + /- 6 yrs ) , old healthy ( n = 20 , 68 + /- 7 yrs ) , and old subjects with mild
peripheral vascular disease (
PVD ) ( n = 8 , 72 + /- 4 yrs ) . -
pubmed
Hemoglobin oxygen saturation was measured as the difference in absorption at 760 and 850 nm light using near - infrared spectroscopy ( NIRS ) . -
pubmed
Effect of
epinephrine on
86Rb efflux ,
45Ca outflow and
insulin release from pancreatic islets perifused in the presence of
propranolol . -
pubmed
In the presence of 16.7 mM
D - glucose , however ,
epinephrine lowered both 86Rb and
45Ca outflow , this coinciding with suppression of
insulin release . -
pubmed
These findings are compatible with the recent proposal that
epinephrine interferes with the entry of
Ca2 + into the B - cell , as mediated by voltage - sensitive
Ca2 + channels , but do not rule out a multifactorial coupling between the occupancy of
alpha 2 - adrenergic receptors and the eventual inhibition of
insulin release . -
pubmed
The
enzyme can convert both
cephalosporin acid to
7 - aminocephalosporanic acid . -
pubmed
Although
IgM and
IgG antibodies to
cholesterol were detected , the titers of
IgG antibodies were low when compared with
IgM. -
pubmed
The anti -
cholesterol vaccine , which consists of liposomes heavily laden with
cholesterol as an antigen and
lipid A as an adjuvant , induces antibodies that react with
low density lipoproteins (
LDL ) and opsonize them for removal by liver macrophages . -
pubmed
Physiological , biochemical , and
EPR characterization of site - directed mutants lacking the highest or lowest potential [
4Fe-4S ] clusters . -
pubmed
The C196A and C227A mutations resulted in the full loss of the four
Fe - S clusters and of the
Mo - cofactor , leading to inactive
enzymes . -
pubmed
The
EPR analysis showed that the highest redox potential [
4Fe-4S ] cluster ( center 1 ) was selectively removed by the C263A mutation and that the C26A replacement likely eliminated the lowest potential [
4Fe-4S ] cluster ( center 4 ) . -
pubmed
In both mutants , the three remaining
Fe - S clusters kept the same spectral and redox properties as in the wild type
enzyme . -
pubmed
Cadmium - detoxification in the earthworm Enchytraeus : specific expression of a putative
aldehyde dehydrogenase . -
pubmed
Northern blot analysis shows that the
Ebaldh - expression is strongly enhanced by
Cd , but remains unaffected by other stressors such as
Zn ,
Hg , and
H2O2 . -
pubmed
buchholzi requires low intracellular concentrations of
aldehydes which are known to target
sulfhydryl groups thus inactivating the
Cd - binding capacity of
CRP . -
pubmed
subtilis
DnaA protein was localized in the 93 C - terminal
amino acids . -
pubmed
VE was measured at peak inspiratory pressures (
PIP ) of 20 , 30 , 40 or 50 cm
H2O while respiratory rate ( RR ) was held constant at 20 breaths / min . -
pubmed
VE was measured as RR was set at 20 , 30 , 40 , or 50 breaths / min while target
PIP was held constant at 20 cm
H2O. -
pubmed
They showed a preference for the binding to
iron - loaded
hTf . -
pubmed
The effect of
[ 3,4 - di ( 4 - methoxyphenyl )- 5 - isoxazolyl ] acetic acid (
mofezolac ) , a potent and highly preferential inhibitor of
cyclooxygenase-1 , and
N -[ 2 -( cyclohexyloxy )- 4 - nitrophenyl ] methanesulphonamide (
NS-398 ) , a selective inhibitor of
cyclooxygenase-2 , on DNA fragmentation and
cyclooxygenase-2 mRNA expression was weak compared to the effect of
indomethacin or
sodium diclofenac . -
pubmed
The DNA fragmentation induced by
sodium diclofenac was hardly affected by the exogenous addition of
16,16 - dimethyl prostaglandin E2 but was inhibited by
caspase inhibitors such as
Ac - YVAD - CHO and
Ac - DEVD - CHO . -
pubmed
The present data provide the first evidence that NSAIDs , such as
indomethacin and
sodium diclofenac , cause apoptotic DNA fragmentation in cultured gastric mucosal cells , and also indicate the involvement of
caspases rather than the inhibition of cellular
prostaglandin synthesis in the apoptotic process . -
pubmed
The accumulation of
cGMP and the production of
TNF - alpha were inhibited by
NG - monomethyl - L - arginine (
L - NMMA ) , indicating that
sCD21 activates the
L - arginine pathway of
NO production . -
pubmed
We demonstrated that
sCD21 activates
NO synthase (
NOS ) since it was found to enhance the conversion of
L - arginine into
L - citrulline and induce the intracellular expression of
inducible NOS in
CD23 + monocytes . -
pubmed
The increases depended on extracellular
Ca2 + , were unaffected by the
inositol 1,4,5 - trisphosphate (
IP3 ) inhibitor
heparin or an
anti - IP3 receptor antibody and were unchanged when the endoplasmic reticulum was segregated to the hemisphere opposite the injection site by centrifugation . -
pubmed
Postnatal
depression ' was suggested in one - third of the sample , and in two - thirds of those with'Type
II '
bulimia , and was predicted by
alcohol misuse , symptom severity and persistence . -
pubmed
hPT cells retained
GSH - dependent enzyme activities during primary culture , whereas cells that had undergone subsequent passage exhibited a loss of activities of most
GSH - dependent enzymes and no longer expressed
P450s or
GSTs . -
pubmed
CYP4A11 expression in primary cultures of hPT cells was significantly increased after treatment for 48 h with either
ethanol ( 50 mM ) or
dexamethasone ( 7 nM ) . -
pubmed
Experiments were performed in the absence of
sodium ; the presence of 2.5 mM
amiloride , a potent inhibitor of the
Na (+)/ H (+ ) antiport ; in the absence of
bicarbonate ; and in the presence of
4 , 4 '- diisothiocyanatodihydrostilbene-2,2 '- di - sulfonic acid ,
disodium salt (
H ( 2 ) DIDS ) for all three activation methods . -
pubmed
This suggests that the
Na (+)/ H (+ ) antiport and the
HCO ( 3 )(-)/ Cl (- ) exchangers are not playing a role during treatment with
calcium ionophore or
thimerosal , and the pH increase observed during treatment with 7 %
ethanol may be dependent upon a
sodium or
bicarbonate flux ( or both ) into the oocyte . -
pubmed
Bafilomycin A1 ( 500 nm ) , an inhibitor of
vacuolar - type H (+ ) ATPases , had no effect on 7 %
ethanol or
thimerosal treatments , but significantly reduced the increase in intracellular pH observed during
calcium ionophore treatment . -
pubmed
There is evidence for accelerated
amino acid substitution in
Hex - t1 that has lost residues known to be associated with
glucose and
glucose-6 - phosphate binding . -
pubmed
The enzyme
5 - lipoxygenase catalyzes the rate - limiting first two steps in the synthesis of
leukotrienes from
arachidonic acid . -
pubmed
The regulation of this movement of soluble
5 - lipoxygenase between the cytoplasm and the nucleoplasm , as well as its impact on
5 - lipoxygenase action ,
leukotriene synthesis and cell function , is only now being elucidated . -
pubmed
Breakthrough times of cartridges and canisters were examined by generating
cyclohexane vapor in a test airflow of 30 L / min in comparison with
CCl4 vapor under various test conditions with different vapor concentrations and relative humidities (
RH ) . -
pubmed
The purpose of the present study was to test the hypothesis that
fatty acid chain length and degree of unsaturation differ with regard to their influence on the postprandial release of cholecystokinin (
CCK ) and the subsequent increase in plasma
bile acid concentrations that occur following a meal . -
pubmed
The high - fat meals were enriched in
oleate or
palmitate , or
linoleate or medium - chain
triacylglycerols (
MCT ) or a blend of
oleate and
long - chain n-3 fatty acids . -
pubmed
The postprandial increase in plasma
CCK concentration was lower on the
MCT meal compared with all meals and was greater following the
linoleate compared with the low - fat meal . -
pubmed
Plasma
bile acid concentrations increased 2 - 3 - fold postprandially but the increase was lower following the
MCT meal compared with the other meals and was greater on the
linoleate meal compared with the low - fat meal . -
pubmed
In conclusion , meals rich in
linoleate are a potent stimulus for
CCK release and lead to prolonged elevations of plasma
bile acids and meals containing
MCT inhibit
CCK release and the subsequent increase in plasma
bile acid concentrations . -
pubmed
Antisense
oligonucleotides to
SYT -
SSX mRNA rapidly and drastically decreased
cyclin D1 and subsequently inhibited cell growth . -
pubmed
The decrease in
cyclin D1 expression , which occurred shortly after inhibition of
SYT -
SSX expression , was found to be primarily dependent on an increased degradation of the
cyclin D1 protein , as assessed by
pulse - chase experiments using
[ ( 35 ) S ] methionine . -
pubmed
The promoters of the
xynA and
xynB genes have been sequenced ; both present consensus sequences for the binding of the
carbon catabolite repressor
CreA , but otherwise show substantial differences . -
pubmed
In contrast ,
xynB is broadly induced by all these
carbon sources ; this may be related to the presence of several
XlnR boxes . -
pubmed
The first enzyme in the biosynthetic pathway of most fructans is
sucrose :
sucrose fructosyl transferase (
SST ) . -
pubmed
Soluble
invertase activity found in the
SST preparations was less sensitive to
KCl and more sensitive to
choline chloride than was
SST . -
pubmed
CuZnSOD is an efficient catalyst of rHCaBP S - nitrosation , and the mechanism of
CuZnSOD - catalyzed S - nitrosation involves reduction of the active - site
Cu ( II ) by a number of the five free
thiols in
rHCaBP , giving rise to
thiyl radicals . -
pubmed
S - nitrosation is rapidly gaining recognition as a major form of protein posttranslational modification , and the efficient S - nitrosation of
CaBP by
CuZnSOD /
GSNO is speculated to be of neurochemical importance given that
CaBP and
CuZnSOD are abundant in neurons . -
pubmed
We examined the potential possibility of an interaction between
nicotine , a major component of cigarette smoke , and
angiotensin II (
Ang II ) , which plays an important role in the pathogenesis of
cardiovascular diseases characterized by
Ang II type 1 ( AT1 ) receptor - mediated abnormal growth of vascular smooth muscle cells ( VSMC ) and fibroblasts . -
pubmed
The
nicotine - induced DNA synthesis was not affected by
valsartan , an
AT1 receptor - specific blocker , or
PD123319 , an
Ang II type 2 ( AT2 ) receptor - specific antagonist . -
pubmed
Nicotine or
Ang II stimulation rapidly increased
extracellular signal - regulated kinase (
ERK ) activation ,
tyrosine- and
serine - phosphorylation of
signal transducer and activator of transcription ( STAT ) 1 and
STAT3 , and
p38 mitogen - activated protein kinase (
p38 MAPK ) , in both cell types . -
pubmed
Interestingly , co - administration of
nicotine and
Ang II at lower doses , which did not affect cell growth , induced DNA synthesis and c - fos expression accompanied by enhancement of
ERK ,
STAT , and
p38MAPK activity . -
pubmed
PD98059 , a
mitogen - activated protein kinase /
ERK kinase inhibitor , or
SB23058 , a
p38MAPK inhibitor , significantly attenuated the vasotrophic effect of
nicotine and
Ang II . -
pubmed
CONCLUSIONS : These results suggest that
nicotine exerts a growth - promoting effect on vascular cells and enhances the
Ang II - induced vasotrophic effect , which is at least partly mediated by the activation of
ERK ,
STAT , and
p38MAPK . -
pubmed
Although
TRP14 contains five
cysteines , only the two
Cys residues in its WCPDC motif were exposed and redox sensitive . -
pubmed
Biochemical characterization of
TRP14 suggested that , like
Trx1 ,
TRP14 is a
disulfide reductase ; its active site
cysteine is sufficiently nucleophilic with the pK(a ) value of 6.1 ; and its redox potential ( -257 mV ) is similar to those of other cellular
thiol reductants . -
pubmed
However , although
TRP14 reduced small
disulfide - containing peptides , it did not reduce the
disulfides of known
Trx1 substrates ,
ribonucleotide reductase ,
peroxiredoxin , and
methionine sulfoxide reductase . -
pubmed
Brevetoxins were detected by radioimmunoassay in methanolic extracts of blood within 1 h
post intraperitoneal ( ip ) administration . -
pubmed
The analytes were then identified and quantitatively determined by gas chromatography - mass spectrometry (
GC -
MS ) via on - line derivatization in the injection - port using a large - volume ( 10 microl ) sample injection with
tetrabutylammonium (
TBA ) salts . -
pubmed
Sixty minutes after the administration of the beverage , the
angiotensin I - induced pressor response decreased to +45+/-7 mmHg at 60 min ( p<0.05 ) , whereas no significant change was detected in the
norepinephrine - induced pressor response . -
pubmed
Familial hemiplegic migraine (
FHM ) is a rare monogenic subform caused by mutations in the
calcium channel gene
CACNA1A or the
Na (+)/ K (+)- ATPase gene
ATP1A2 . -
pubmed
Anti -
CD20 monoclonal antibody (
rituximab ) in the treatment of
pemphigus . -
pubmed
Arachidonic acid [ AA , 20:4(n-6 ) ] ,
EPA [ 20:5(n-3 ) ] , and
DHA [ 22:6(n-3 ) ] antagonized the depression of
LDLr gene expression by
25OH - cholesterol and increased
LDLr protein abundance 1- to 3 - fold ( P & lt ; 0.05 ) , but had no significant effects on
LDLr mRNA levels . -
pubmed
Oleic ( 18:1 ) ,
linoleic ( 18:2 ) , and
alpha - linolenic acids [ 18:3(n-3 ) ] did not significantly affect
LDLr expression . -
pubmed
The present results suggest that AA ,
EPA , and
DHA increase
LDLr protein levels , and that
ACAT plays a role in modulating the effects of AA on
LDLr protein levels . -
pubmed
Furthermore , the effects of the
fatty acids appeared to be independent of any change in
SREBP-1 protein . -
pubmed
Of these 5,437 ( 79.5 % ) showed
sinus rhythm (
SR ) and 1,398 ( 20.5 % ) showed
AF . -
pubmed
CONCLUSIONS :
BioGlue appears to be an effective adjunct in preventing postoperative
CSF leaks after transsphenoidal surgery . -
pubmed
Supramolecular metal ion assemblies are deposited from their solutions onto highly orientated pyrolytic
graphite ( HOPG ) substrates to be imaged by scanning tunnelling microscopy (
STM ) . -
pubmed
The renal antioxidant enzymes such as
superoxide dismutase (
SOD ) ,
catalase (
CAT ) ,
glutathione peroxidase (
GPx ) activities and level of
reduced glutathione (
GSH ) were declined ; level of
malondialdehyde (
MDA ) was elevated in the
cisplatin alone treated group . -
pubmed
CONCLUSIONS :
Granulocyte colony - stimulating factor (
G - CSF ) ,
all - trans retinoic acid (
ATRA ) , and vaccines met 2 of 3 criteria for an association with
SS . -
pubmed
There are sufficient data and a plausible pharmacologic mechanism for
G - CSF and
ATRA . -
pubmed
Our analysis showed MELD score ,
bilirubin ,
ALT levels and white cell counts preorthotopic liver transplantation are important predictive factors for outcome . -
pubmed
Accumulation of
hypoxia - inducible factor-1alpha through a novel electrophilic ,
thiol antioxidant - sensitive mechanism . -
pubmed
15 - deoxy - Delta ( 12,14 )- prostaglandin - J ( 2 ) (
15d - PGJ ( 2 ) ) is a
peroxisome - activated proliferator receptor - gamma (
PPARgamma ) agonist which contains an alpha , beta - unsaturated electrophilic
ketone involved in nucleophilic addition reactions to
thiols . -
pubmed
15d - PGJ ( 2 ) induced stabilization of
HIF-1alpha protein , without affecting
HIF-1alpha mRNA levels or proteasome activity , leading to its nuclear accumulation and activation of
HIF - induced transcription . -
pubmed
Accumulation of
HIF-1alpha was unaffected by selective
PPARgamma blockade nor mimicked by the
PPARgamma agonists
ciglitazone and
9,10 - dihydro-15d - PGJ ( 2 ) . -
pubmed
agents that act as
thiol reducing agents and/or increase the
GSH content ) , but not reactive
oxygen species ( ROS ) scavengers , prevented
15d - PGJ ( 2 )-induced
HIF-1alpha accumulation whereas the inhibitor of
GSH synthesis
buthionine sulfoximine cooperated with
15d - PGJ ( 2 ) to accumulate
HIF-1alpha . -
pubmed
Finally ,
HIF-1alpha expression was increased by the electrophilic
alpha , beta - unsaturated compounds acrolein and
PGA ( 2 ) , but not by
9,10 - dihydro-15d - PGJ ( 2 ) , which lacks the electrophilic
cyclopentenone moiety . -
pubmed
Taken together , these results point out to a new mechanism to increase pharmacologically the cell levels of
HIF-1alpha through the electrophilic reaction of
alpha , beta - unsaturated ketones with
thiol groups . -
pubmed
The primary efficacy end point was glycemic control as determined by changes in
glycosylated hemoglobin (
HbA(1c ) ) from baseline to study end between those receiving combination
MER+S treatment and those receiving
sulfonylurea monotherapy . -
pubmed
There were significant decreases from baseline in mean fasting plasma
glucose ( FPG ) levels by the end of week 1 and in mean
HbA(1c ) levels by week 8 in each
MER+S group ( both , P & lt ; 0.001 ) . -
pubmed
The mean ( 95 % CI ) changes from baseline to study end in the combined MER+S groups (
HbA(1c ) , -0.74 % [ -0.85 % to -0.64 % ] ; FPG , -12.9 [ -17.1 to -8.7 ] mg / dL ) were significantly different from the
sulfonylurea monotherapy group (
HbA(1c ) , 0.08 % [ -0.08 % to 0.25 % ] ; FPG , 15.5 [ 8.2 to 22.8 ] mg / dL ; P & lt ; 0.001 ) . -
pubmed
The unadjusted 6 - month medical costs for the newer agents ranged from $ US 2715 for
paroxetine CR to $ US 6042 for
duloxetine . -
pubmed
Remarkable seasonal consistency was found in the amount of storage reserves available for nocturnal carboxylation , a consequence of predominantly daytime export of
carbohydrate in
spring and summer whilst nocturnal export was the major sink for
carbohydrate in autumn and winter . -
pubmed
In this study , the following topics regarding SN identification in
lung cancer patients will be introduced : ( 1 ) devices for SN identification using a radioisotope tracer ; ( 2 ) movement of
Tc-99 tin colloid after injection ; ( 3 ) characteristics of patients in whom SNs could not be identified ; ( 4 ) results of ex vivo SN identification ; ( 5 ) reliability of in vivo SN identification ; ( 6 ) algorithm for reducing mediastinal lymph node dissection ; ( 7 ) SN identification using SPECT /
CT ; ( 8) differences in SN identification between large and small radioisotope particles ; ( 9 ) size of metastatic and nonmetastatic mediastinal lymph nodes in
non - small cell lung cancer ; ( 10 ) SN navigation segmentectomy for clinical stage IA
non - small cell lung cancer ; and ( 11 ) lymphatic flow at segmental lymph nodes . -
pubmed
The K(i ) results reveal a surprising possibility that
VKOR in some bird species are easily inhibited by
warfarin . -
pubmed
At day 28 , the proportion of
CD34 + cells , myocardial
BrdU immunohistochemical stain , myocardial capillary density ,
myocardial fibrosis and cardiac function was detected among the 3 groups . -
pubmed
RESULTS : In Group G , the proportion of
CD34 + cells and cardiac function was significantly higher than groups N and C , and
BrdU positive cells in myocardium were higher than those other groups . -
pubmed
RESULTS : We found that dietary supplementation of 10 g
MLP kg(-1 ) or 1 g Q3MG kg(-1 ) in high - fat diet effectively suppressed blood
glucose levels . -
pubmed
Moreover , the production of
chemokine - containing HS - TEX could be inhibited by
ATP inhibitor ,
calcium chelator , and
cholesterol scavenger , indicating that the mobilization of
chemokines into exosomes was
ATP- and
calcium - dependent and via a lipid raft - dependent pathway . -
pubmed
Socio - demographic information was collected via a self - administered instrument ( gender , age , school grade , economic level ( EL ) and family - head 's EL ) , SBC for
PA , behavioural factors ( smoking ,
alcohol and stress ) and
PA barriers . -
pubmed
Modeling the kinetics of
digoxin absorption : enhancement by
P - glycoprotein inhibition . -
pubmed
An increase in the area under the curve ( AUC ) after oral
digoxin due to coadministration of drugs known as
P - glycoprotein (
P - gp ) inhibitors has been reported in several studies , but there is very little information on the rate of absorption after
P - gp inhibition . -
pubmed
However , even the effect of the highest dose of
naltrexone ( 20 mg / kg ) was incomplete , which is in contrast to the effect of the
NMDA receptor antagonist
MK-801 , which has been tested in the same model and found to completely prevent the incidence of
allodynia at a dose of 0.5 mg / kg . -
pubmed
Thus ,
NMDA receptor antagonists may contribute to the prevention of
tissue damage by antagonizing the excitotoxic action of
glutamate and/or
aspartate released by
ischemia into the spinal cord . -
pubmed
Catalytic enantioselective one -
pot aziridoarylation reaction of
aryl cinnamyl ethers has been demonstrated in detail . -
pubmed
trans-3 - Amino-4 - arylchroman is an advanced intermediate for the synthesis of
chromenoisoquinoline compounds such as
doxanthrine , a potent and selective full agonist for the
dopamine - D ( 1 ) receptor . -
pubmed
Thrombin generation in
PPP was assessed using calibrated automated thrombogram . -
pubmed
TiO2 nanoparticles induce
insulin resistance in liver - derived cells both directly and via macrophage activation . -
pubmed
We hypothesized that despite being considered " inert " as bulk material ,
TiO ( 2 ) NPs may impair
insulin responses in liver - derived cells , either indirectly by inflammatory activation of macrophages , and/or by directly interfering with
insulin signaling . -
pubmed
Furthermore , direct exposure of Fao cells to
TiO ( 2 ) results in activation of the stress kinases
JNK and
p38MAP kinase , and in induction of
insulin resistance at the signaling and metabolic levels . -
pubmed
Total
collagen and
testosterone levels peaked at 12 months of age . -
pubmed
Breeds were ranked identically for total
collagen and
testosterone levels at 12 months . -
pubmed
The increased synthesis of
collagen appears to be influenced by
testosterone or some other event associated with puberty . -
pubmed
When stratified by
PSA level of & lt;=10 ng / mL vs. & gt;10 ng / mL , Gleason score , or
androgen deprivation therapy , no statistically significant differences in bPFS were discerned between the two EBRT regimens . -
pubmed
The homogeneous alignments of helical rod - like
polysilanes on a rubbed
polyimide alignment layer were investigated by polarized optical microscopy ( POM ) and atomic force microscopy (
AFM ) analyses . -
pubmed
The POM and
AFM observations determined that
polysilanes with a series of aliphatic side chains helically arranged around the main chains were tilted to the right and left by 33 from the rubbing direction when the handedness of the side - chain helical array is left and right , respectively . -
pubmed
Infrared ( IR ) and nuclear magnetic resonance ( NMR ) spectroscopy have revealed that semi - fluorinated (
SF ) acids containing a
perfluorooctyl group (
C8F17 ) as a terminal segment exist also as
hydrogen - bonded dimers , which are the units of inter- and intramolecular movements in their liquids and
CCl4 . -
pubmed
In fact , low intramolecular movements for
SF - acid were confirmed by
13C - NMR
T1 measurements . -
pubmed
Immuno fluorescent studies also revealed the extracellular filamentous
actin organization over the scaffolds , where cell adhesion and proliferation was found to be higher with increase in
PGA content , which is a hydrophilic polymer . -
pubmed
Against this background ,
estrogen - alone effects on joint symptoms were examined in
post hoc analyses in the Women 's Health Initiative randomized , placebo - controlled , clinical trial . -
pubmed
Spearman 's rank correlation was used to determine the association of the five anthropometric indices with metabolic parameters including fasting plasma
glucose ,
triglyceride ,
high density lipoprotein and blood pressure . -
pubmed
Sesterterpene glycinyl - lactams : a new class of
glycine receptor modulator from Australian marine sponges of the genus Psammocinia . -
pubmed
Bioassay guided fractionation of three southern Australian marine sponges of the genus Psammocinia , selected for their ability to modulate
glycine - gated chloride channel receptors (
GlyRs ) , yielded the rare marine
sesterterpenes ( -)-
ircinianin ( 1 ) and
( -)- ircinianin sulfate ( 2 ) , along with the new biosynthetically related metabolites
( -)- ircinianin lactam A ( 3 ) ,
( -)- ircinianin lactam A sulfate ( 4 ) ,
( -)- oxoircinianin ( 5 ) ,
( -)- oxoircinianin lactam A ( 6 ) and
( -)- ircinianin lactone A ( 7 ) . -
pubmed
The discovery and characterization of
sesterterpenes 1 - 8 , and in particular the
glycinyl - lactams 3 and 6 , provide valuable new insights into
GlyR pharmacology . -
pubmed
We focus our attention on multiple repeats of one
amino acid ( homorepeats ) and create a new database ( named
HRaP , at http://bioinfo.protres.ru/hrap/ ) of occurrence of homorepeats and disordered patterns in different proteomes . -
pubmed
HRaP is aimed at understanding the
amino acid tandem repeat function in different proteomes . -
pubmed
SH3P2 also binds to
phosphatidylinositol 3 - phosphate (
PtdIns3P ) and functions downstream of the
phosphatidylinositol 3 - kinase (
PtdIns3 K ) complex . -
pubmed
Our study aimed to investigate whether
PKC (
protein kinase C)-
NF - kappaB (
nuclear factor kappaB ) is involved in
CCL2 - induced regulation of voltage - gated
sodium Nav1.8 currents and expression . -
pubmed
Compared with the control , significant change in
Nav1.8 current density was observed when the
CCR2 inhibitor
INCB3344 ( 10 nM ) was applied . -
pubmed
Furthermore , inhibition of
PKC by
AEB071 significantly eliminated
CCL2 - induced elevated
Nav1.8 currents . -
pubmed
Moreover ,
p65 expression was significantly higher in
CCL2 - induced neurons ( P<0.05 ) , and was reversed by treatment with
INCB3344 and
AEB071 . -
pubmed
CONCLUSIONS : Our results suggest that
Glycine max may change the expression level of
CYPs , especially
CYP3A4 and
CYP1A 7 , involved in biotransformation of xenobiotics ( drugs , procarcinogens ) and may participate in clinically significant interactions with drugs metabolized by these enzymes . -
pubmed
Additional studies indicated the production of reactive
oxygen species accompanied of decreases in
vitamin E and
GSH contents and of
glutathione transferase activity . -
pubmed
Estrogens are known that they may produce
breast cancer by actions on
ER and also as chemical
carcinogens , as a consequence of their oxidation leading to reactive metabolites . -
pubmed
Duplex staining for
proliferating cell nuclear antigen and
alpha - smooth muscle actin (
alpha - SMA ) , as well as
MTT and flow cytometry assays , were performed to measure fibroblast proliferation . -
pubmed
Curcumin treatment inhibited fibroblast proliferation and the cell cycle was arrested in the
G1 phase . -
pubmed
Curcumin treatment upregulated the expression of
PPAR - gamma and downregulated the expression of
p - Smad2 / 3 . -
pubmed
These results suggest that
curcumin treatment ameliorates
renal fibrosis by reducing fibroblast proliferation and ECM accumulation mediated by
PPAR - gamma and
Smad - dependent
TGF - beta1 signaling . -
pubmed
Crustins are cationic
cysteine - rich antimicrobial peptides ( AMPs ) that contain multiple domains (
glycine - rich ,
cysteine - rich , or
proline - rich ) at the N - terminus and whey acidic protein (
WAP ) domains at the C - terminus . -
pubmed
The synthesized WAP domain of
MjCru I-1 but not synthesized
Cys - rich domain has antibacterial and agglutinative activities . -
pubmed
During the first stage
ethanol is oxidized into
acetaldehyde , under the action of
alcohol dehydrogenase . -
pubmed
Micro - agar larval development test ,
MTT test of
cytotoxicity , and biotransformation study coupled with Ultra high performance liquid chromatography - tandem mass spectrometry ( UHPLC -
MS /
MS ) technique were used for this purpose . -
pubmed
The objective of this study was to increase the potency and durability of vaccine - induced immunity against
B cell lymphoma by combining
alpha - galactosylceramide (
alpha - GalCer)-loaded
tumor cell vaccination with an agonistic antibody targeting the immune checkpoint molecule
4 - 1BB (
CD137 ) . -
pubmed
RESULTS : Aerobic training significantly increased peak
oxygen uptake ( VO2 ) ( 18.1 + - 3.0 vs. 22.9 + - 2.8 mL / kg / min ) , decreased the change in muscle
oxygen saturation from
rest to submaximal and peak exercise ( SmO2 ; 2.4 + - 5.7 vs. -7.0 + - 3.4 % ) , and increased the relative change in deoxygenated
hemoglobin /
myoglobin concentration from
rest to submaximal ( -1.5 + - 2.3 vs. 3.0 + - 3.6 mumol / L ) and peak exercise ( 1.1 + - 4.5 vs. 8.2 + - 3.5 mumol / L ) . -
pubmed
HSCT using alternative donors with
post - transplantation
cyclophosphamide ( PT / Cy ) for
GVHD prophylaxis has been performed for
hematologic malignancies with engraftment ,
GVHD , and TRM comparable with that seen with
HLA - matched related donors . -
pubmed
Furthermore , separation and detection of underivatized optical isomers of
amphetamine ,
cathinone ,
nor - mephedrone , and
pregabalin using UFCE /
MS can be achieved with an analysis time of less than two minutes . -
pubmed
Resolutions of 1.3 , 3.7 and 3.8 were achieved for
pregabalin ,
cathinone and
nor - mephedrone , respectively , under UFCE /
MS conditions . -
pubmed
The samples were extracted once with
acetonitrile , concentrated by evaporation and injected into the LC -
MS /
MS system . -
pubmed
Dual Energy
CT Pulmonary Angiography with 6 g
Iodine - A Propensity Score - Matched Study . -
pubmed
CONCLUSION : Dual - energy
CTPA with advanced monoenergetic reconstruction is feasible with 6 g
iodine and allows for the diagnosis and safe exclusion of central , lobar , and segmental
PE . -
pubmed
Infusion reactions during
infliximab treatment are not associated with
IgE anti - infliximab antibodies . -
pubmed
OBJECTIVES : Controversy exists on the role of
IgE antidrug antibodies (
IgE - ADA ) in infusion reactions ( IR ) on
infliximab treatment , partly due to the lack of a positive control used for assay validation . -
pubmed
Kissorphin (
KSO ) is a new peptide derived from
kisspeptin-10 . -
pubmed
Moreover , prior administration of the
NPFF receptor antagonist
RF9 ( 10 nmol/300 muL , i.v . ) -
pubmed
Simulated bioavailability of
phosphorus from aquatic macrophytes and phytoplankton by aqueous suspension and incubation with
alkaline phosphatase . -
pubmed
Haloacetic Acid Water Disinfection Byproducts Affect
Pyruvate Dehydrogenase Activity and Disrupt Cellular Metabolism . -
pubmed
Previous studies of
monoHAA - induced
genotoxicity and cell stress demonstrated that the toxicity was due to inhibition of
glyceraldehyde-3 - phosphate dehydrogenase (
GAPDH ) , leading to disruption of cellular metabolism and energy homeostasis . -
pubmed
In this study , we evaluated the effects of
mono - , di - , and tri - HAAs on cellular
GAPDH enzyme kinetics , cellular
ATP levels , and
pyruvate dehydrogenase complex (
PDC ) activity . -
pubmed
The
monoHAAs ,
iodoacetic acid and
bromoacetic acid , were the strongest inhibitors of
GAPDH and greatly reduced cellular
ATP levels . -
pubmed
Chloroacetic acid ,
diHAAs , and
triHAAs were weaker inhibitors of
GAPDH and some increased the levels of cellular
ATP . -
pubmed
HAAs also affected
PDC activity , with most
HAAs activating
PDC . -
pubmed
The primary finding of this work is that mono- versus multi -
HAAs address different molecular targets , and the results are generally consistent with a model in which
monoHAAs activate the
PDC through
GAPDH inhibition - mediated disruption in cellular metabolites , including altering
ATP - to -
ADP and
NADH - to -
NAD ratios . -
pubmed
The
monoHAA - mediated reduction in cellular metabolites results in accelerated
PDC activity by way of metabolite - ratio - dependent
PDC regulation . -
pubmed
DiHAAs and
triHAAs are weaker inhibitors of
GAPDH , but many also increase cellular
ATP levels , and we suggest that they increase
PDC activity by inhibiting
pyruvate dehydrogenase kinase . -
pubmed
Different techniques have been used to manage
PAC - induced
pulmonary artery rupture , including double lumen endotracheal tube (
DLT ) , bronchial blockers , pulmonary artery embolization , thoracotomy with
hematoma evacuation , and extracorporeal life support for ventilation ( ECLS ) . -
pubmed
The suitability of the device for bioanalysis and bioimaging was demonstrated by monitoring the degradation of a thin
poly ( ester amide ) film with the enzyme
alpha - chymotrypsin using LAPS . -
pubmed
In this work , the interactions between
MT2 - MMP and common trivalent metal ions , including
aluminum (
Al3 + ) and
ferrum (
Fe3 + ) ions , were investigated . -
pubmed
Enzymatic detection revealed that
Al3 + and
Fe3 + strongly inhibited the
MT2 - MMP . -
pubmed
Fluorescence spectrography elucidated a static quenching interaction between the negatively charged
amino acids on
MT2 - MMP and the inhibitory trivalent metal ions , indicating that a stable complex was formed between
MT2 - MMP and metal ions . -
pubmed
Dosage forms of Genentech
BCS class
II drugs ,
GDC-0810 ,
GDC-0941 , and compound A , were tested by starting the dissolution in either pH 1.6 or pH 4.0 media then converting to FaSSIF after 30 min . -
pubmed
One
pot ligand exchange method for a highly stable
Au - SBA-15 catalyst and its room temperature
CO oxidation . -
pubmed
Using the method of beta - cells detection with antibodies to
insulin , we demonstrated that
streptozotocin reduced the number of beta - cells and impaired their morphological structure . -
pubmed
However ,
shikimate is not catalytically involved in the reactions of the
monolignol pathway , and is only needed for pathway
enzymes to recognize their main substrates . -
pubmed
After
CS exposure , tissue slides were made from uterine and ovarian samples and examined after
hematoxylin and eosin staining . -
pubmed
Furthermore , during oral
glucose and
insulin tolerance tests , FO from
dyslipidaemic dams exhibited altered
glucose tolerance and
insulin sensitivity ( P & lt ; 0.05 ) when compared with FO from CTL dams . -
pubmed
Switching to
bupropion was strongly dominated by
bupropion augmentation at an
ICER of -$640 / remission ( 95 % CI , -$5,770 to $ 3,008 ) . -
pubmed
The
ICER for the
aripiprazole augmentation versus switching to
bupropion was $ 1,074 / remission ( 95 % CI , $ 47 to $ 5,022 ) , and the
ICER for
aripiprazole augmentation versus
bupropion augmentation was $ 5,094 / remission ( 95 % CI , -$34,027 to $ 32,774 ) . -
pubmed
In such conditions ,
rocuronium remains able to cause
immunoglobulin E cross - linkage and the
anaphylaxis mechanism can continue . -
pubmed
Intradermic skin tests confirmed
rocuronium immunoglobulin E - mediated
anaphylaxis but also showed intradermal injection of mixing in equal molecular ratio of
sugammadex with
rocuronium preventing
rocuronium anaphylactic skin reaction . -
pubmed
Based on the physics understanding of the resistive switching , the measured
NDR behavior is believed to be associated with the partially unipolar reset effect , which is due to the recombination between
oxygen vacancies and the thermally released
oxygen ions from the
RuO2 interface layer . -
pubmed
The
nitrate reductase activity was the highest in the C+ N grown cells and the lowest activity was found in the
glutamate grown cells . -
pubmed
[ Role of
PPAR - gamma - regulated autophagy in
genistein - induced inhibition of hepatic stellate cell activation ] . -
pubmed
OBJECTIVE : To investigate the inhibitory effect of
genistein on activation of hepatic stellate cells ( HSCs ) in vitro and the role of the autophagy pathway regulated by
PPAR - gamma in mediating this effect . -
pubmed
The cells pretreated with
3 - MA prior to
genistein treatment showed significantly increased protein expressions of -SMA and
1 ( I ) collagen compared with the cells treated with
genistein only ( P & amp;lt ; 0.05 ) . -
pubmed
CONCLUSIONS :
PPAR - gamma- regulated autophagy plays an important role in mediating
genistein - induced inhibition of HSC activation in vitro . -
pubmed
Triglyceride deposit cardiomyovasculopathy (
TGCV ) is a phenotype primarily reported in patients carrying genetic mutations in
PNPLA2 encoding
adipose triglyceride lipase (
ATGL ) which releases long chain
fatty acid (
LCFA ) as a major energy source by the intracellular
TG hydrolysis . -
pubmed
Patients with I - TGCV , of which etiologies and causes are not known yet , suffered from adult - onset severe
heart disease , including
heart failure and
coronary artery disease , associated with a marked reduction in
ATGL activity and myocardial washout rate of
LCFA tracer , as similar to those with P - TGCV . -
pubmed
Discovery of a Lead
Triphenylethanamine Cholesterol Ester Transfer Protein (
CETP ) Inhibitor . -
pubmed
Lead optimization of the
diphenylpyridylethanamine (
DPPE ) and
triphenylethanamine (
TPE ) series of
CETP inhibitors to improve their pharmaceutical profile is described . -
pubmed
RESULTS : Our data showed that
EXE promoted hepatic autophagy via activation of canonical autophagy signaling pathways via mediating
microtubule - associated protein B - light chain 3 II (
LC3 - II ) ,
autophagy protein 7 (
ATG7 ) , phosphorylated
adenosine mono phosphate - activated protein kinase ( p -
AMPK ) ,
CATHEPSIN L ,
lysosome - associated membrane protein 2 (
LAMP2 ) , and a reduction in
p62 . -
pubmed
Compared with the CON group , the ITM group showed decrease in ( P < 0.05 ) egg production , eggshell strength , eggshell palisade layer , palisade layer ratio , serum
estrogen ,
luteinizing hormone , glycosaminoglycan concentration , and
carbonic anhydrase activity and increase in ( P < 0.05 ) egg loss and mammillary layer ratio . -
pubmed
In this study ,
polystyrene microplastic particles (
PSMPs ) with a size of 0.047 mum were selected as the representative of microplastics . -
pubmed
Soil physicochemical properties play important roles in sorption of
PSMPs , especially the content of soil organic
carbon . -
pubmed
The sorption capacity was positively correlated with soil organic
carbon (
R2 = 0.999 ) and
Fe2O3 content (
R2 = 0.967 ) , while the sorption of clay showed opposite trend (
R2 = 0.952 ) . -
pubmed
The origin of
PNH is the somatic mutation in the X - linked
phosphatidylinositol glycan class A (
PIG - A ) gene located on Xp22 : this condition leads to the production of clonal blood cells with a deficiency in those surface proteins that protect against the lytic action of the activated complement system . -
pubmed
In the presence of heat stress , the intracellular
IAA content showed a significant increase in the two
LeDnaJ07 overexpression strains but only a slight change in the YS55 wild type
strain . -
pubmed
OBJECTIVES : We established a novel mitochondrial / cytosolic purification pipeline for complimentary
GC -
TOF -
MS and
1H - NMR metabolomics using robust , commercially available fractionation strategies . -
pubmed
Apoptosis and intracellular reactive
oxygen species ( ROS ) induced by
PLF were accessed using high - content analysis with florescent staining . -
pubmed
The ability of
PLF to scavenge free radicals was evaluated by
2,2 - diphenyl-1 - picrylhydrazyl (
DPPH ) assay . -
pubmed
Nlrp3 inflammasome elicit out an inflammatory response by emission of pro inflammatory cytokines by ROS ( reactive
oxygen species ) production , mobilization of
K + efflux and
Ca2 + and by activation of lysosome destabilization that eventually causes pyroptosis , a programmed cell death process . -
pubmed
Morphine-3 - glucuronide upregulates
PD - L1 expression via
TLR4 and promotes the immune escape of
non - small cell lung cancer . -
pubmed
M3 G activated the
PI3 K and the
NFkappaB signaling pathways , and this effect was antagonized by a
TLR4 pathway inhibitor . -
pubmed
Repeated
M3 G intraperitoneal injections promoted LLC
tumor growth and lung
metastasis through the upregulation of
tumor expressed
PD - L1 and the reduction of CTL in the
tumor microenvironment . -
pubmed
Conclusions :
M3 G specifically activated
TLR4 in
NSCLC cells and upregulated
PD - L1 expression through the
PI3 K signaling pathway , thereby inhibiting CTL cytotoxicity and finally promoting
tumor immune escape . -
pubmed
In this study , the influence of headspace pressure (
HP ; 20 - 100 kPa ) and organic loading rate ( OLR ; 10 - 30 g / L ) on
H2 and volatile
fatty acid ( VFA ) yield were investigated . -
pubmed
More than 79 % generated
H2 was consumed through homoacetogenesis pathway when
HP was 100 kPa , and lowing
HP could effectively promote the accumulation of
H2 ( increased by at least 2 times ) . -
pubmed
Even though consumed
H2 through homoacetogenesis was related to OLR and
HP , the lower
HP was more likely to reduce this part
H2 consumption , especially under 10 g / L condition . -
pubmed
VIP - induced lipolysis and
adenyl cyclase activity are not affected by
phenoxybenzamine . -
pubmed
VIP - induced lipolysis is inhibited by
propranolol but
VIP - induced
adenyl cyclase activity is not . -
pubmed
Triphenylmethane dyes as inhibitors of
reverse transcriptase ,
ribonucleic acid polymerase , and protein synthesis . -
pubmed
A comparison between skin tests , inhalation tests , specific
IgE and basophil
histamine release measurements . -
pubmed
Nonspecific
phosphatase activity and
Ca2 + -dependent
ATPase activity were not inhibited by Con A treatment of the cells . -
pubmed
Abnormal
lipoprotein - lipase - mediated plasma
triglyceride removal in untreated
diabetes mellitus associated with
hypertriglyceridemia . -
pubmed
An abnormality in the interaction of
lipoprotein lipase with endogenous circulating plasma lipoprotein
triglyceride has been demonstrated in untreated
diabetes . -
pubmed
These
diabetics have a decreased maximal removal capacity for plasma
triglyceride ( 27.0 mg
TG / kh / hr ) and increased Km ( 390 mg / dl ) for endogenous plasma
triglyceride -
lipoprotein lipase interaction compared to that found in nondiabetic
hypertriglyceridemic subjects ( Vmax , 32.0 ; km , 157 ) . -
pubmed
The presence of free
intrinsic factor in the small intestine has considerable physiological significance in maintaining the enterophepatic circulation of
vitamin B12 . -
pubmed
The exact role of ovarian sex
steroids in the neuroendocrine regulation of
thyrotropin ( TSH ) release in women can only be accurately assessed in the absence of any considerable ovarian sex
steroid feedback upon the hypothalamic - pituitary unit . -
pubmed
Compared to the control conditions , serum
E2 and P4 concentrations markedly increased ( p less than 0.001 ) following oral
E2 or
E2 / P4 treatments . -
pubmed
Total
triiodothyronine ( T3 ) and
thyroxine ( T4 ) concentrations and free T3 and T4 equivalents remained unchanged during
E2 and
E2 / P4 regimens . -
pubmed
Since the
TSH pulse attributes (
pulse amplitudes , frequencies , interpulse intervals , mean
TSH concentrations , by Cluster
pulse algorithm ) did not significantly change during
E2 and
E2 / P4 replacements , the episodic character of
TSH secretion virtually remained unchanged by sex
steroid replacements. -
pubmed
Effects of
calcium and
potassium extracellular ionic concentration changes on the hippocampal
CA1 activity of purinergic drugs . -
pubmed
The data demonstrate a direct intrinsic activity of
potassium ions , and suggest a possible direct interaction between
adenosine receptors and
potassium transneuronal fluxes . -
pubmed
Phorbol ester and
phospholipase C - mediated differentiated thyroid function in vitro : the effects of
protein kinase C inhibition and downregulation . -
pubmed
,
12 - O - tetradecanoylphorbol 13 - acetate (
TPA ) , inhibit
TSH - stimulated
iodide organification in vitro implying a role for
protein kinase C (
PKC ) in the regulation of differentiated thyroid function . -
pubmed
To further explore the
PKC dependence of this action of
TPA , we studied the effects of
PKC inhibition and downregulation on
phorbol - mediated differentiated thyroid function in vitro . -
pubmed
Exogenous
PLC ( 1 U / mL ) could mimic these effects of
TPA . -
pubmed
The
PKC inhibitor ,
1 -( 5 - isoquinolinesulfonyl )- 2 - methylpiperazine ( H7 ) inhibited
TSH - stimulated
iodide organification at concentrations exceeding 10 microM. -
pubmed
After 24 h of
TPA treatment to induce
PKC downregulation , no recovery of TSH - stimulated
iodide organification was observed , suggesting that the effects of
TPA were irreversible . -
pubmed
These studies indicate that the effects of
TPA and
PLC on differentiated thyroid function are mediated , at least in part , by
PKC . -
pubmed
Intracerebroventricular infusions of
insulin ( 4 and 40 micrograms / min for 30 min ) increased plasma
AVP concentrations dose - dependently without
hypoglycaemia , but decreased hypothalamic and pituitary contents of
AVP . -
pubmed
In the presence of 5 mM
glucose the
preproinsulin mRNA content was increased by 10(-8 ) M
Met - enkephalin to 198 % ( p less than 0.001 ) and by
Leu - enkephalin to 145 % ( p less than 0.001 ) of the control without enkephalins . -
pubmed
The
preproinsulin mRNA content was raised in the presence of 5 mM
glucose by 10(-10 ) M significant . -
pubmed
The biosynthesis of ( pro)
insulin was strongly decreased by
actinomycin D at the
glucose and
enkephalin concentrations tested . -
pubmed
The role of the
amino acid in position 31 of
cholecystokinin CCK26 - 33 in the recognition of central and peripheral receptors was investigated by replacement of
methionine-31 by
amino acids with side chains of various chemical nature . -
pubmed
Interestingly , when the
amine function in the side chain of the
ornithine-31 was protected by a
benzyloxycarbonyl group , an unusual high affinity for pancreatic binding sites was observed and the related analogue proved to be a new peripheral
CCK antagonist . -
pubmed
After 4 hours incubation in
potassium cyanide and
2 - deoxyglucose , there was a 43 % increase in
beta - adrenergic receptor number , 41 % decrease in
adenosine triphosphate , and minimal morphological change in myocytes . -
pubmed
Thus :
beta - adrenergic receptor density increases during relatively early stages of
injury in metabolically impaired myocytes with reduced
adenosine triphosphate levels and decreases subsequently , after the myocytes become irreversibly injured ; the increased
beta - adrenergic receptor density in moderately injured myocytes can be reversed upon removal of the injurious agent and restoration of the cellular
adenosine triphosphate level ; and changes in
catecholamines mediated by an intact nervous system are not required for an increase in
beta - adrenergic receptor density in the setting of impaired energy metabolism . -
pubmed
An acute stress , produced by crowding and confinement for 30 min , increased
ACTH - IR approximately 10 - fold , and plasma
cortisol levels 50 - fold , but the plasma
alpha - MSH level was not affected . -
pubmed
The
amino acid substitution did not interfere with the synthesis , processing , and stability of the
env gene polypeptides gp120 and
gp41 or the binding of
gp120 to its cellular receptor , the
CD4 ( T4 ) molecule . -
pubmed
Molecular characterization of two revertant proviral clones revealed the presence of the original mutation as well as a compensatory
amino acid change in another region of
HIV gp120 . -
pubmed
The peak of
plasma vasopressin level occurs at the same time as the late in vivo effect of
aldosterone on passive
22Na efflux from arterial smooth muscle . -
pubmed
Several hormones induce
phosphatidylinositol turnover in cell membranes and thus activate
protein kinase C. -
pubmed
Utilizing in vitro microperfusion techniques , we found that activation of
protein kinase C with either
PMA or
L - alpha-1,2 - DOG significantly inhibited net
sodium absorption , net
potassium secretion and transepithelial voltage in a dose - dependent manner . -
pubmed
[ The
HL - A system of transplantation
antigens ] . -
pubmed
Evidence from proton magnetic resonance data for the stacking of
aromatic amino acids in
lysine - vasopressin : comparison with
oxytocin derivatives and related
dipeptides . -
pubmed
Dopamine receptor antagonism by the novel antianxiety drug ,
buspirone . -
pubmed
The reduction of
cytochrome P-450 - -
CO complex in the presence of various agents in the reconstituted system of liver cell organelles was studied . -
pubmed
The addition of
glutamate ( but not
succinate ) ,
NAD + and
amytal ( or
rotenone ) to the reconstituted system caused a 40 - 50 % reduction of
NADPH - reducible cytochrome P-450 . -
pubmed
The inhibitor of mitochondrial
NADH - cytochrome b5 reductase dicumarol prevented the
cytochrome P-450 reduction in the presence of
glutamate ,
NAD + and
amytal but did not affect the reduction of
cytochrome P-450 by the added
NADH . -
pubmed
It was concluded that the electron transfer from the
NAD - dependent substrates of the inner mitochondrial respiratory chain to the microsomal
cytochrome P-450 occurs with the participation of non - bound
NAD and
cytochrome b5 of the outer mitochondrial membrane on the condition that the membranes of the two main oxidative systems are in tight contact . -
pubmed
The effect of different antidepressants (
MAO inhibitors ,
monoamine uptake inhibitors , new drugs ) on the activity of
bovine brain monoamine oxidase was investigated . -
pubmed
The type A
MAO activity was found to be selectively inhibited by the
morpholine derivative ,
moclobamide ( IC50 - 10(-5 ) M ) . -
pubmed
When injected in the presynaptic neuron ,
syntaxin complementary RNA also depressed
Ca2 + current and transmission . -
pubmed
Saturation analysis also showed that
Ang II ( 0.1 nM ) increased the KD value of
[ 3H ] PAC in the WKY
strain but in contrast decreased the KD value of
[ 3H ] PAC in the SHR . -
pubmed
Hypothiocyanite production was detected in two
strains and it was only detected in these
strains after growth in
MRS medium in aerobic conditions . -
pubmed
It may be concluded that relaxations elicited by hypercapnic
acidosis are associated with a fall of extracellular pH and an activation of the electrogenic
Na + pump , and those caused by hyperosmolarity are due to stimulation of the Na
(+)-H
+ exchange and the Na+ pump . -
pubmed
Discrete nuclei and other regions of the brain are solubilized in concentrated
guanidine thiocyanate solution , mRNA is directly hybridized with riboprobes , and detected with a
ribonuclease protection assay . -
pubmed
RESULTS :
N - beta - phenylethyl lidocaine was found to be a potent
Na + channel blocker in vitro . -
pubmed
Exceptionally stable
salt bridges in
cytochrome P450cam have functional roles . -
pubmed
A long - standing puzzle in structure - function studies of
cytochrome P450cam is how the substrate ,
camphor , reaches the buried active site . -
pubmed
Strikingly , of the four most stable salt links in the
protein ( delta Gtot & lt ; -6 kcal / mol ) , two involve the
propionate groups of the
heme and the other two involve
Asp251 . -
pubmed
Thus it appears that
cytochrome P450cam has evolved four unusually strong salt bridges , stabilized by surrounding charged and polar groups in the protein , to keep its
heme cofactor in place and to regulate substrate binding . -
pubmed
A total of 929 lines belonging to 8 vibrational bands , 1 - 0 to 8 - 7 , for
Na35 Cl , 252 lines of 1 - 0 , 2 - 1 , and 3 - 2 bands of
Na37 Cl , and 355 lines of 1 - 0 , 2 - 1 , 3 - 2 , and 4 - 3 bands of
K35 Cl have been measured and combined with the existing microwave and millimeter - wave data to obtain a set of refined molecular constants . -
pubmed
12 - O - tetradecanoylphorbol-13 - acetate (
TPA ) binds to and activates
PKC . -
pubmed
Ovulation , endometrial thickness , serum
oestradiol and
progesterone , urinary
luteinizing hormone , endometrial morphology and cervical mucus were assessed . -
pubmed
Effects of
poly ( ADP - ribose ) polymerase inhibition on cell
death and
chromosome damage induced by
VP16 and
bleomycin . -
pubmed
We demonstrate that in lymphoblasts
VP16 treatment induces apoptosis to a greater extent than
BLM treatment , and that
PARP inhibition reduces
VP16 - induced apoptosis whereas it has no effect on
BLM - induced apoptosis . -
pubmed
Hence ,
PARP appears to play a significant role in
VP16 - induced apoptosis and not in
BLM - induced apoptosis . -
pubmed
ap3 - 1 contains a single nucleotide change in the
AP3 gene that alters both an
amino acid in the
AP3 protein and the 5 ' splice consensus site for intron 5 . -
pubmed
PURPOSE :
Brimonidine is a highly selective
alpha2 - adrenoreceptor agonist that lowers intraocular pressure . -
pubmed
Numerous
topoisomerase I poisons including DNA minor groove binders such as
Hoechst 33258 and DNA intercalators such as
benzophenanthridine alkaloids and
indolocarbazole derivatives have been discovered and developed . -
pubmed
Many of these
topoisomerase I suppressors are natural products (
beta - lapachone ,
diospyrin ,
topostatin ,
topostin ,
flavonoids ) which are believed to interact directly with the enzyme . -
pubmed
Endothelial NO synthase (
eNOS ) generated
NO ( 140 + /- 20 nmol l(-1 ) ) in lung during the acute phase ( first 10 - 15 min ) of
endotoxemia , followed by production of
NO by inducible
NOS . -
pubmed
In spite of
cortisol replacement at 17 years ,
insulin requirement fell further to 0.25 U / kg / day at 18 years . -
pubmed
The up - regulation of
survivin expression may be due to the concurrent rise in
progesterone concentrations during the normal menstrual cycle . -
pubmed
Hematoxylin - eosin , Masson 's trichrome , and immunohistochemical stainings for
Ulex europaeus I lectin binding ,
CD31 , and
type IV collagen were done to differentiate lymphatic and blood vessels and to demonstrate basement membranes in vascular walls and around
carcinoma cell nests was observed . -
pubmed
Skeletal muscle
glucose transport , plasma
insulin ,
systolic blood pressure , and plasma lipids were measured in all groups after 22 months . -
pubmed
In the HFS group ,
insulin - stimulated
glucose transport was significantly reduced ( 67+/-4 versus 98+/-4 pmol . -
pubmed
15 s(-)(1 ) ) , whereas plasma
insulin ( 300+/-49 versus 82+/-8 pmol / L ) , blood pressure ( 147+/-4 versus 123+/-4 mm Hg ) , plasma
triglycerides ( 2.58+/-0.31 versus 0.39+/-0.04 mmol / L ) ,
LDL cholesterol ( C ) ( 3.45+/-0.40 versus 0.89+/-0.06 mmol / L ) ,
LDL - C to
HDL - C ratio ( 2.9+/-0.1 versus 2.2+/-0.1 ) ,
VLDL - C ( 1.53+/-0.23 versus 0.37+/-0.07 mmol / l ) , Total - C ( 5.56+/-0.58 versus 1.49+/-0.10 mmol / L ) , and
body weight ( 360+/-11 versus 260+/-5 g ) were all significantly elevated compared with the LFCC . -
pubmed
Conversion to a LFCC diet for 2 months led to normalization of
glucose transport , blood pressure , plasma
insulin , and
VLDL - C and significant amelioration of
obesity and other
lipid abnormalities . -
pubmed
Metallothionein (
MT ) is a low - molecular - weight
cysteine - rich protein , which has the ability to bind and sequestrate heavy metal ions . -
pubmed
Synthesis of
MT is induced in a variety of tissues by these metal ions , as well as by endogenous factors such as glucocorticoids ,
interferon ,
interleukin-1 and
vitamin D. -
pubmed
As plasma proteins , including
alpha-1 - acid glycoprotein (
AGP ) , may reduce drug efficacy through binding ,
AGP was investigated for its ability to interact with
STI571 . -
pubmed
Thus , these in vitro findings suggest that
AGP will not abrogate the antileukemic activity of
STI571 . -
pubmed
Heparin cofactor II (
HCII ) is a plasma protein that inhibits
thrombin rapidly in the presence of
dermatan sulfate ,
heparan sulfate , or heparin . -
pubmed
This alpha - LBD antibody showed high agonist activity for anti - apoptosis ( 56 + /- 9 % relative to
PTN ) , low agonist activity for cell growth ( 21 + /- 1 % relative to
PTN ) , and was an antagonist of
PTN - induced cell growth ( 61 + /- 2 % inhibition ) . -
pubmed
Specifically , we used
pertussis toxin (
PTX ) as a tool to investigate the relationship between
cocaine - induced alterations in
cAMP signaling and behavior . -
pubmed
After behavioral testing , brains were removed and processed for in vitro receptor autoradiography using the
D ( 1 ) receptor ligand [
3H ]
SCH 23390 . -
pubmed
These findings suggest a role for inhibitory proteins (
G ( i )/ G ( o ) ) within the nucleus acumbens in locomotor activity and also
cocaine - induced behavioral sensitization . -
pubmed
The
benzaldehyde derivatives were more potent inhibitors of
CYP2A5 than the
phenylethylamines . -
pubmed
The K(ic ) value of
4 - methylbenzaldehyde was 3.4 micro M and for
4 - methoxybenzaldehyde it was 0.86 micro M for
CYP2A5 . -
pubmed
Amphetamine is a weak inhibitor of
CYP2A6 , whereas
benzaldehyde is a suicide inhibitor with K(inact ) = 0.16 min(-1 ) and K(I ) = 18 micro M. The K(ic ) values of
2 - phenylethylamine ,
2 -( p - tolyl )- ethylamine ,
4 - methylbenzaldehyde and
4 - methoxybenzaldehyde were 1.13 , 0.23 , 0.36 and 0.73 micro M for
CYP2A6 , respectively . -
pubmed
Novel potent inhibitors were found for
CYP2A6 and , except for
4 - methoxybenzaldehyde , all the compounds inhibited
CYP2A5 and
CYP2A6 enzymes differentially . -
pubmed
AIP1 binds to the C - terminal domain of
ASK1 via a
lysine - rich cluster within the N - terminal
C2 domain . -
pubmed
STUDIES ON
GLYCYRRHIZIN , AN ACTIVE PRINCIPLE OF RADIX
LIQUIRITINE (
II ) INHIBITION BY
GLUCOSACCHAROLACTONE OF DETOXYCATING ACTION OF
GLYCYRRHIZIN . -
pubmed
THYROXINE - BINDING BY THE
THROXINE - BINDING GLOBULIN AS A MEASURE OF THE ANABOLIC ACTIVITY OF CONGENERS OF
TESTOSTERONE . -
pubmed
These data provide genetic evidence for the existence of tonically active
MOR and
DOR systems that modulate basal
DA neurotransmission in the NAc . -
pubmed
Furthermore , they demonstrate that in contrast to the locomotor - activating effects of
morphine , a small component of
morphine - evoked DA release occurs independently of
MOR activation . -
pubmed
Using the Chirobiotic T (
teicoplanin ) chiral stationary phase , native and derivatized
theanine enantiomers were separated and detected via high - performance liquid chromatography ( HPLC ) coupled to atmospheric pressure ionization mass spectrometry ( API -
MS ) . -
pubmed
Optimum sensitivity and detection limits for derivatized
theanine standards were achieved using ESI -
MS . -
pubmed
The effective doses of
ICRP74 were higher than those of KORMAN with percent differences ranging from 6 % ( LLAT , 10 MeV ) to 30 % (
PA , 0.05 MeV ) . -
pubmed
AEA binds to the
cannabinoid receptor CB1 , located at the central nervous system , while
OEA is an endogenous ligand for the alpha subtype of
peroxisome - proliferator activating receptor (
PPARalpha ) . -
pubmed
Besides typical central effects of
cannabinoids ,
CB1 receptor activation leads to
hyperphagia , whereas its pharmacological blockade is followed by changes in energy metabolism favouring substrate oxidation . -
pubmed
OEA has inhibitory effects on food intake by acting on
PPARalpha receptors which modulate the autonomous nervous system . -
pubmed
Peptides obtained by proteolysis of the purified
enzyme were isolated , sequenced and used to prepare degenerate oligonucleotide primers . -
pubmed
The predicted
amino acid sequence contains type
II transmembrane region , three potential N - glycosylation sites as well as all of the isolated peptide sequences . -
pubmed
The Vmax of the recombinant
enzyme , 2.08 micromol/(min mg ) , was nearly the same as the native
enzyme , 2.12 micromol/(min mg ) , when assayed with partially deglycosylated
mucins as
glycosyl acceptors . -
pubmed
These results show for the first time that multiple isoforms of
GalNAc transferases acting in sequence may be required for the complete O - glycosylation of
mucin polypeptide chains , and those acting on the nacent polypeptide chain synthesize intermediates which can serve as
glycosyl acceptors for other isoforms of the
enzyme . -
pubmed
EPR studies of
chlorocatechol 1,2 - dioxygenase : evidences of
iron reduction during catalysis and of the binding of amphipatic molecules . -
pubmed
We determined the zero - field splitting of the
Fe ( III ) cofactor ( D = 1.3 + /- 0.2 cm(-1 ) ) by electron paramagnetic resonance (
EPR ) experiments that along with other structural data allowed us to infer the
Fe ( III ) coordination environment . -
pubmed
Protein assays show that the
enzyme activity is significantly lowered in the presence of
stearic - acid molecules . -
pubmed
Crystal structures of all five compounds show short intramolecular asymmetric
hydrogen bonds (
SHB ) between adjacent
carboxyl groups with O ... O distance average of 2.40 A. -
pubmed
The vibrational modes , mainly
SHB modes , are tentatively assigned by molecular orbital ab initio calculations of
pyromellitic acid and anions
[ C6H2 ( COO ) 4H3 ]- and
[ C6H2 ( COO ) 4H2 ] 2 - . -
pubmed
The detected
ERBB2 mutation showed G to C transversion at bp 2305 ( 2305G>C ) , which would result in the substitution of
Asp to
His at codon 769 ( D769H ) . -
pubmed
The
amino acid D769 is located in the alpha - helix within the kinase domain , which is important in the binding of
ATP with
ERBB2 . -
pubmed
In addition , we detected considerable
NF2 promoter activity in various
NF2 - affected tissues such as acoustic
ganglion , trigeminal
ganglion , spinal ganglia , optic chiasma , the ependymal cell - containing tela choroidea , and the
pigmented epithelium of the retina . -
pubmed
This article describes recent developments in sex - related differences in opioid (
morphine ) pharmacodynamics ,
morphine metabolites , the
nociceptin / orphanin FQ receptor system , acute opioid tolerance and opioid - induced side - effects , such as opioid - induced
respiratory depression and
itch , and
P - glycoprotein modulation of opioid effect . -
pubmed
The structure , texture , and electronic properties of nanocomposites were studied using various characterization techniques such as
N2 physisorption , X - ray diffraction ( XRD ) , laser Raman spectroscopy ( LRS ) , temperature - programmed reduction (
TPR ) , transmission electron microscopy (
TEM ) , and X - ray photoelectron spectroscopy ( XPS ) . -
pubmed
TEM images reveal that the pores of
SBA-15 are progressively blocked with
MnO ( x ) nanoparticles . -
pubmed
The surface structures of
MnO ( x ) were also determined by LRS , XPS , and
TPR . -
pubmed
Finally , the micropatterned
FAS - SAM was immersed in a solution of 1 vol %
( aminoethylaminomethyl ) phenethyltrimethoxysilane (
AEAMPS ) in absolute
toluene . -
pubmed
As a control , the micropatterned hydrophobic
FAS - SAM template ( composed of
CF3- and
OH - terminated surfaces ) was also treated under the same conditions . -
pubmed
Optical microscopy and atomic force microscopy confirmed that the formation of the continuous mesocomposite film proceeded only on the
FAS - SAM - covered regions , while the
AEAMPS - SAM - covered regions remained free of deposits . -
pubmed
SCA - alpha2 , a close mimic of the metabolite , NCSi - gb , of the DNA bulge - specific
enediyne antibiotic
neocarzinostatin , differs in possessing a more stable spirocyclic ring system and in lacking certain bulky groupings that compromise bulged DNA binding . -
pubmed
Like NCSi - gb ,
SCA - alpha2 possesses the natural
N - methylfuranose moiety , alpha - linked to the
benzindanol ( BI ) moiety . -
pubmed
Lacking the bulky
cyclocarbonate of NCSi - gb , the SCA-alpha2.bulged DNA complex has a much less twisted and buckled 3'-bulge - flanking base pair ( dG20.dC8 ) , and the
G20 residue stacks directly above the BI ring platform . -
pubmed
G - CSF , prophylactic antibiotics , and
mesna were provided . -
pubmed
Hemoglobin , red blood cell count , hematocrit , serum
vitamin A , urinary
iodine and prevalence of
angular stomatitis were measured at baseline and at the end of the study after one year . -
pubmed
RESULTS : There was a significant ( p<0.05 ) improvement in the experimental group in
hemoglobin , red cell count , urinary
iodine and serum
vitamin A whereas in the control group there was a statistically significant decline ( p<0.05 ) in
hemoglobin , hematocrit , red cell count and urinary
iodine . -
pubmed
Furthermore , experiments using the glutamatergic antagonist
CNQX indicated that
GA probably binds to non -
NMDA receptors . -
pubmed
We also provided some evidence that
3 - OHGA competes with the
glutamate transporter inhibitor
L - trans - pyrrolidine-2,4 - dicarboxylate , suggesting a possible interaction of
3 - OHGA with
glutamate transporters on synaptic membranes . -
pubmed
( 3 ) These results indicate that
glutamate binding to receptors and transporters can be inhibited by
GA and
3 - OHGA in cerebral cortex and striatum in a developmentally regulated manner . -
pubmed
smegmatis mutants of the lipoprotein - encoding
lpqW gene have a profound defect in
LAM biosynthesis . -
pubmed
Here we show that restoration of
LAM biosynthesis in the
lpqW mutant results from secondary mutations in the
pimE gene . -
pubmed
Our findings provide the first strong evidence that
LpqW is required to channel intermediates such as
AcPIM4 into
LAM synthesis and that loss of
PimE function results in the accumulation of
AcPIM4 , bypassing the need for
LpqW. -
pubmed
Polarization of natural killer T cells towards an NKT2 subpopulation occurs after stimulation with
alpha - galactosylceramide and
rhG - CSF in
aplastic anemia . -
pubmed
In summary , our results indicate that polarization of NKT cells towards the NKT2 subpopulation occurs after co - stimulation with
alpha - galactosylceramide and
rhG - CSF in
AA . -
pubmed
The
malondialdehyde (
MDA ) levels and activities of
superoxide dismutase (
SOD ) and
catalase (
CAT ) were measured and the number of apoptotic neurons was counted . -
pubmed
diminuta did not affect the degree of
gastric ulceration at either 3 or 7 days
post -
acetic acid application , as gauged by
ulcer area or histopathology . -
pubmed
Both
kinases are key drivers of osteoclast maturation , and three agents that block osteoclastogenesis , the
Src inhibitor
dasatinib , the
bisphosphonate alendronate , and the osteoclast - specific apoptosis - inducer
reveromycin A , markedly reduced
17 - AAG - stimulated
tumor growth in bone . -
pubmed
Resistance to ESAs : A hyporesponse to ESAs is considered to be present when an
Hb level of 11 g / dl is not achieved with a dose of
epoetin & gt ; 300 IU / kg / week or a dose of
darbepoetin alpha & gt ; 1.5 microg / kg / week ( Strength of Recommendation B ) . -
pubmed
The L - channel agonist
Bay K8644 increased the magnitude of both step and tail currents , but surprisingly failed to slow deactivation of
TS channels . -
pubmed
Roscovitine , a
tri - substituted purine that enhances L - channel OSvdI , restored TS - impaired
OSvdI. -
pubmed
The cell surface
intelectin-1 was not a
glycosylphosphatidylinositol - anchored membrane protein . -
pubmed
We demonstrate that exposure of the skin to
calcipotriol before transcutaneous immunization with
OVA protein and CpG adjuvant prevents
Ag - specific
CD8(+ ) T cell priming coincident with Langerhans cell depletion in the skin . -
pubmed
Immunization through
calcipotriol - treated skin induces
CD4(+)
CD25(+ ) regulatory T cells ( Treg ) that prevent subsequent Ag - specific
CD8(+ ) T cell proliferation and
IFN - gamma production . -
pubmed
Topical
calcipotriol treatment also induces
RANKL (
receptor activator of NF - kappaB ligand ) expression by keratinocytes , a
TNF family member involved in modulation of skin dendritic cells . -
pubmed
The in vivo expansion of Ag - specific Treg with the topical application of the
vitamin D analog
calcipotriol followed by transcutaneous immunization is a simple method to augment functional Ag - specific
CD4(+)
CD25(+)
Foxp3(+ ) Treg populations and mimics
Ag - specific UV - induced tolerance . -
pubmed
Chemoenzymatic elaboration of
monosaccharides using engineered
cytochrome P450BM3 demethylases . -
pubmed
Demethylases displaying high levels of regioselectivity toward a number of protected
monosaccharides were identified using a combination of protein and substrate engineering , suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry , biology , and medicine . -
pubmed
The relation of
serum parathyroid hormone and serum
calcium to serum levels of
prostate - specific antigen : a population - based study . -
pubmed
Experimental and clinical data implicate
calcium and
parathyroid hormone (
PTH ) in the development of
prostate cancer . -
pubmed
We examined the relationship between serum levels of
calcium ,
PTH , and
prostate - specific antigen (
PSA ) , an established marker of prostate growth , in a large , population - based study using multivariate linear regression . -
pubmed
Adjusted for age , race ,
body mass index , and serum levels of
25 - hydroxyvitamin D , serum levels of
PTH were significantly positively correlated with serum
PSA ( P = 0.01 ) . -
pubmed
Serum levels of
PTH and
calcium each were correlated significantly with free
PSA ( P = 0.05 and 0.008 , respectively ) . -
pubmed
These findings support the hypothesis that serum
calcium and serum
PTH stimulate prostate growth in men without clinical
prostate cancer and have implications for the use of
PSA as a screening tool for
prostate cancer . -
pubmed
Comparative
16S rRNA gene stable isotope probing (
SIP ) in the presence and absence of
sulfate indicated that a Desulfosporosinus species , which constitutes only 0.006 % of the total microbial community
16S rRNA genes , is an important
sulfate reducer in a long - term experimental peatland field site . -
pubmed
Parallel
SIP using
dsrAB ( encoding
subunit A and B of the dissimilatory ( bi ) sulfite reductase ) identified no additional
sulfate reducers under the conditions tested . -
pubmed
The link of
DAP1 to autophagy was first apparent in that its knockdown enhanced autophagic flux and in that it displayed a rapid decline in its phosphorylation in response to
amino acid starvation . -
pubmed
Mapping of the phosphorylation sites and analysis of phosphorylation mutants indicated that
DAP1 is functionally silenced in growing cells through
mTOR - dependent phosphorylations on
Ser3 and
Ser51 . -
pubmed
We found that exposure to
genistein did not affect circulating levels of the
androgen 11 - ketotestosterone or the
estrogen E2 relative to negative - control fish . -
pubmed
Caveolin 1 is required for the activation of
endothelial nitric oxide synthase in response to
17beta - estradiol . -
pubmed
Evidence suggests that
estrogen mediates rapid
endothelial nitric oxide synthase (
eNOS ) activation via
estrogen receptor - a (
ERalpha ) within the plasma membrane of endothelial cells ( EC ) . -
pubmed
However , the role played by
caveolin 1 in regulating
17beta - estradiol - mediated
NO signaling in EC has not been adequately resolved . -
pubmed
Thus , the purpose of this study was to explore how
17beta - estradiol stimulates
eNOS activity and the role of
caveolin 1 in this process . -
pubmed
Further , before
estrogen stimulation
ERalpha associates with
caveolin 1 , whereas stimulation promotes a
pp60(
Src)-mediated phosphorylation of
caveolin 1 at
tyrosine 14 , increasing
ERalpha -
PI3 kinase interactions and disrupting
caveolin 1 -
ERalpha interactions . -
pubmed
Furthermore ,
Y14FCav overexpression reduced
eNOS phosphorylation at
serine1177 and decreased
NO generation after
estrogen exposure . -
pubmed
Delivery of a synthetic peptide based on this sequence decreased
ERalpha plasma membrane translocation and reduced
estrogen - mediated activation of
eNOS . -
pubmed
In conclusion ,
caveolin 1 stimulates
17beta - estradiol - induced
NO production by promoting
ERalpha to the plasma membrane , which facilitates the activation of the
PI3 kinase pathway , leading to
eNOS activation and
NO generation . -
pubmed
The catalytic reduction of
NO ( 2 ) with
hydrogen on a
Pt field emitter tip is investigated using both field electron microscopy ( FEM ) and field ion microscopy (
FIM ) . -
pubmed
One -
pot conversions of
olefins to
cyclic carbonates and
secondary allylic and homoallylic amines to
cyclic carbamates . -
pubmed
Sequential treatment of a
1,2 - disubstituted olefin with
m - CPBA ,
Br3CCO2H , and
DBU results in the one -
pot , stereospecific conversion of the
olefin to the corresponding
disubstituted cyclic carbonate (
1,3 - dioxolan-2 - one ) . -
pubmed
Tyrosinase is the key and rate - limiting enzyme responsible for the conversion of
tyrosine into melanin . -
pubmed
DeoxyArbutin not only shows greater inhibition of
tyrosinase activity but is also safer than
hydroquinone and
arbutin . -
pubmed
The expression of
mtc was induced after exposure to all metals , except for one
chromium concentration . -
pubmed
Exposure to soil originating from metal - contaminated field sites also induced
mtc , while the expression did not change in response to a
polycyclic aromatic hydrocarbon . -
pubmed
Furthermore , we determined the effects of increasing extracellular
K (+ ) concentrations ( [ K(+)](o ) ) and inhibition of
Na (+) , K (+)- ATPase on neurogenic and
norepinephrine - induced contractile responses . -
pubmed
The neuromodulatory effects of
PGEs were tested in the presence of
K (+ ) channel blockers . -
pubmed
The results demonstrate that
PGE ( 1 ) and
PGE ( 2 ) inhibit adrenergic responses by a prejunctional mechanism that involves the activation of
large - conductance Ca ( 2 +)- activated K (+ ) channels and
Na (+) , K (+)- ATPase . -
pubmed
Leucine - rich
amelogenin peptides regulate mineralization in vitro . -
pubmed
Amelogenin 's capacity to regulate enamel formation is related to its conserved N- and C - terminal domains , its ability to self - assemble , and its ability to stabilize amorphous
calcium phosphate (
ACP ) - a capacity enhanced by
amelogenin phosphorylation . -
pubmed
TEM , selected area electron diffraction , and Fourier transform - infrared spectroscopy were also used to determine the effect of phosphorylated and non - phosphorylated
LRAP on
calcium phosphate formation . -
pubmed
Notably , this capacity was enhanced by added
calcium and to a much greater degree for phosphorylated
LRAP . -
pubmed
Furthermore , phosphorylated
LRAP was found to stabilize
ACP and prevent its transformation to
hydroxyapatite ( HA ) , while aligned HA crystals formed in the presence of non - phosphorylated
LRAP . -
pubmed
The N- and C - terminal
amelogenin domains in non - phosphorylated
LRAP are , therefore , sufficient to guide
ACP transformation into ordered bundles of
apatite crystals , making
LRAP an excellent candidate for biomimetic approaches for enamel regeneration . -
pubmed
Sandblasting abrasion with 50 micro
alumina particles at a specific distance , pressure and time was the only surface treatment in
DT Light Post and Transluma Post that increased the bond strength to dual cure resin composite cores . -
pubmed
We have previously shown that
amphiregulin (
AREG ) , a ligand to the
epidermal growth factor receptor (
EGFR ) , is induced during the repair / differentiation process elicited by
naphthalene - induced
lung injury . -
pubmed
We show that
AREG and epithelial
EGFR expression is dispensable to pulmonary epithelial repair after
naphthalene - induced
lung injury , but regulates secretory cell differentiation to a mucus - producing phenotype . -
pubmed
We show that the pulmonary epithelium is the source of
AREG , suggesting that
naphthalene - induced
MCM is mediated through an autocrine signaling mechanism . -
pubmed
Our data demonstrate that
AREG - dependent
EGFR signaling in airway epithelial cells contributes to
MCM in
naphthalene - induced
lung injury . -
pubmed
Analyses using
MTT assay and measurement of
lactate dehydrogenase release showed that presence of
27 - hydroxycholesterol counteracted the toxic effects of
staurosporine on these cells . -
pubmed
Also ,
27 - hydroxycholesterol significantly decreased the
staurosporine - mediated induction of
caspase-3 and - 7 , known to be important in apoptotic events . -
pubmed
Therefore , the molecular signaling events affecting
resveratrol - mediated
heme oxygenase-1 (
HO-1 ) and
glyoxalase expression levels were further investigated in this study . -
pubmed
Our findings indicated that
resveratrol activated the
extracellular signal - regulated kinase (
ERK ) pathway but not the
p38 or
c - Jun N - terminal kinase (
JNK ) pathways , subsequently leading to
Nrf2 nuclear translocation and elevation of
HO-1 and
glyoxalase expression levels . -
pubmed
The efficacy of agents that alkylate the O-6 position of
guanine is inhibited by
O ( 6 )- alkylguanine - DNA alkyltransferase (
AGT ) which removes these lesions from the
tumor DNA . -
pubmed
A newly synthesized prodrug of the
AGT inhibitor
O ( 6 )- benzylguanine (
O ( 6 )- BG ) with an
alpha , alpha - dimethyl-4 - nitrobenzyloxycarbonyl moiety masking the essential
2 - amino group has demonstrated the feasibility of targeting hypoxic regions that are unique to
solid tumors , for drug delivery . -
pubmed
Recently , new potent global
AGT inhibitors with improved formulatability such as
O ( 6 )-[( 3 - aminomethyl ) benzylguanine ( 1 ) have been developed . -
pubmed
The findings show a significant modulation effect of
cadmium on
H - ras gene expression accompanied by up - regulation of
caspase-3 - related apoptosis in the concentration range of 100 - 1000 nMu cadmium . -
pubmed
Consequently , the blood
calcium , calciotropic hormones , and skeleton are normal at birth despite severe
vitamin D deficiency or genetic deletion of
calcitriol or
vitamin D receptor . -
pubmed
This study compares the in vitro and in vivo properties of
ASD material generated by fast evaporation , lyophilization , and spray - drying methods using
griseofulvin as a model compound and hydroxypropyl methylcellulose acetate succinate as the polymer matrix . -
pubmed
Lesatropane , a synthesized chiral
tropane (
3S , 6S - isomer of satropane ) , is a novel
muscarinic agonist , and is being under preclinical development in China for the treatment of
primary glaucoma . -
pubmed
The reports concerning that activation of
muscarinic acetylcholine receptors (
mAChRs ) could protect cells against apoptosis prompted us to study the neuroprotective effects of
lesatropane and the mechanism . -
pubmed
Atropine or
pirenzepine , antagonist of
mAChR or
M1 mAChR , antagonized the protective effects of
lesatropane respectively and suppressed the
lesatropane 's effects on
ERK1 / 2 . -
pubmed
Furthermore ,
chelerythrine , a
PKC inhibitor , partially suppressed
ERK1 / 2 activation induced by
lesatropane . -
pubmed
The results indicated that the specific
M1 mAChR via
PKC -
ERK1 / 2 pathway might be involved in the neuroprotective effects of
lesatropane . -
pubmed
Palmitic acid induces production of
proinflammatory cytokines interleukin-6 ,
interleukin-1beta , and
tumor necrosis factor - alpha via a
NF - kappaB - dependent mechanism in HaCaT keratinocytes . -
pubmed
Pyrrolidine dithiocarbamate (
PDTC , a selective chemical inhibitor of
NF - kappa B ) and
goat anti - human IL-6 polyclonal neutralizing antibody were used to inhibit
NF - kappa B activation and
IL-6 production , respectively . -
pubmed
Our results showed that
palmitic acid induced an upregulation of
IL-6 ,
TNF - alpha ,
IL-1 beta secretions , accompanied by
NF - kappa B nuclear translocation and activation . -
pubmed
Moreover , the effect of
palmitic acid was accompanied by
PPAR alpha activation and
Stat3 phosphorylation . -
pubmed
Palmitic acid - induced
IL-6 ,
TNF - alpha ,
IL-1 beta productions were attenuated by
NF - kappa B inhibitor
PDTC . -
pubmed
Palmitic acid was administered in amounts able to elicit significant hyperproliferation and can be attenuated by
IL-6 blockage . -
pubmed
These data demonstrate for the first time that
palmitic acid can stimulate
IL-6 ,
TNF - alpha ,
IL-1 beta productions in HaCaT keratinocytes and cell proliferation , thereby potentially contributing to
acne inflammation and
pilosebaceous duct hyperkeratinization . -
pubmed
In
ovo administration of high dose
CORT significantly suppressed the growth rate from 3weeks of age and increased the frequency of
aggressive behaviors , and the dosage was associated with elevated plasma
CORT concentrations and significantly downregulated hypothalamic expression of
arginine vasotocin (
AVT ) and
corticotropin - releasing hormone (
CRH ) . -
pubmed
High dose
CORT exposure significantly increased platelet
serotonin ( 5 - HT ) uptake , decreased whole blood
5 - HT concentration ( p<0.05 ) , downregulated hypothalamic
tryptophan hydroxylase 1 (
TPH1 ) mRNA and upregulated
5 - HT receptor 1A (
5 - HTR1A ) and
monoamine oxidase A (
MAO - A ) mRNA , but not
monoamine oxidase B (
MAO - B ) . -
pubmed
High dose
CORT also significantly increased DNA methylation of the hypothalamic
GR and
CRH gene promoters ( p<0.05 ) . -
pubmed
In marginal structural models ,
IL-1beta ,
IL-6 ,
IL-8 were associated with lower
estradiol and
progesterone concentrations . -
pubmed
Urushiol induced cytostatic cell growth inhibition via upregulation of the
cyclin - dependent kinase inhibitors ,
p21 (
WAF1 /
CIP1 ) and
p27 ( KIP1 ) proteins and down - regulation of
cyclin - dependent kinase 2 and 4 proteins in a
p53 - independent manner . -
pubmed
DNA polymerases engineered by directed evolution to incorporate non - standard
nucleotides . -
pubmed
DNA polymerases have evolved for billions of years to accept natural
nucleoside triphosphate substrates with high fidelity and to exclude closely related structures , such as the analogous
ribonucleoside triphosphates . -
pubmed
However ,
polymerases that can accept unnatural
nucleoside triphosphates are desired for many applications in biotechnology . -
pubmed
This review focuses on experiments that , by directed evolution , have created variants of
DNA polymerases that are better able to accept unnatural
nucleotides . -
pubmed
After a therapeutic
lefetamine dose , the
bis - nor , bis - nor - hydroxy ,
nor - hydroxy ,
nor - di - hydroxy metabolites could be detected using the authors '
GC -
MS SUSA and the
nor - hydroxy - glucuronide by the LC - MS(n ) SUSA . -
pubmed
In immature neurons ,
GABA ( A ) R activation leads to neuronal depolarization and activation of
Ca ( 2 + ) signals , which mediate many of the developmental effects of
GABA . -
pubmed
BRD4 promotes
pancreatic ductal adenocarcinoma cell proliferation and enhances
gemcitabine resistance . -
pubmed
Furthermore , we showed that the expression of
BRD4 was increased after treatment with
gemcitabine (
GEM ) . -
pubmed
Here we report a theoretical study on the binding of two molecules , 05B and
P4O , to the proteins
MK2 and
MK3 . -
pubmed
Acid - resistant proteins , such as
mucins , were identified after pellicle was exposed to
lactic or citric acid . -
pubmed
Further comparison of the MCI and
NC groups revealed that declarative memory was inversely associated with
cortisol levels ( P & lt ; 0.001 ) , but this association became marginal when
25 - hydroxy vitamin D was included in the linear multivariate regression analyses ( P = 0.06 ) . -
pubmed
The
cognitive impairment may be the result of
HPA axis dysregulation but
25 - hydroxy vitamin D serum concentrations might be compensatory or even a potent contributing factor . -
pubmed
Cell cycle was changed in the transformed cells after
EGCG treatment with significantly increased cell numbers in G0 /
G1 phase and decreased cell numbers in S phase compared to control group , P & lt ; 0.001 . -
pubmed
Both mRNA and protein levels of
hTERT gene were significantly decreased in cells after treated with
EGCG , P & lt ; 0.001 . -
pubmed
c - Myc protein level was significantly decreased after
EGCG treatment , especially in the highest dose group ( i.e. -
pubmed
A
silver island film ( SIF ) substrate was used to demonstrate that Metal - Enhanced Fluorescence (
MEF ) is a powerful tool to enable detection of emission from ( bio)molecules at very low concentrations . -
pubmed
The
VEGF concentrations of combination group [ ( 94.61 + - 5.46 ) ng / L decreased significantly , as compared to
thalidomide group [ ( 141.11 + - 3.70 ) ng / L and
IFN group [ ( 119.90 + - 2.00 ) ng / L ( P & lt ; 0.05 ) . -
pubmed
Stabilizing
hypoxia inducible factor-1alpha with
dimethyloxallyl glycine increased early survival , but not proliferation or differentiation , in neurospheres in vitro and in newly born SGZ cells in vivo . -
pubmed
Microbial
phytase - catalyzed
phytate dephosphorylation was accompanied by increased
iron and
zinc release from the cereal substrates . -
pubmed
No increases in soluble
cadmium , lead or
arsenic were observed with microbial
phytase - catalyzed
phytate dephosphorylation . -
pubmed
CONCLUSION : Microbial
phytase treatment abated
phytate chelation hence enhanced the release of
iron and
zinc from
phytate - rich cereals under simulated gastric conditions . -
pubmed
The data infer that acid - stable microbial
phytases can help improve
iron bioavailability from
phytate - rich cereal substrates via
post - ingestion activity . -
pubmed
Uniform
rhodium nanoparticles (
NP ) with three different particle sizes ( 1.9 , 2.4 , and 3.6 nm ) were prepared via a
polyol method with
rhodium ( III ) acetylacetonate ,
poly ( vinylpyrrolidone ) with different concentrations of
sodium citrate . -
pubmed
The recombinant virus DC474 - 480 constructed with
tyrosines 474 , 476 , 477 , and 480 mutated to
alanine residues produced a
gK - null - like phenotype characterized by the production of very small plaques and accumulation of capsids in the cytoplasm of infected cells . -
pubmed
Immunoprecipitation assays revealed that replacement of all four
tyrosines with
alanines substantially reduced the ability of
gK to interact with
UL37 . -
pubmed
Collectively , these results suggest that the
UL37 conserved
tyrosine 480 residue plays a crucial role in interactions with
gK to facilitate cytoplasmic virion envelopment and infectious virus production . -
pubmed
We show here that
UL37 tyrosine residues conserved among all alphaherpesviruses serve critical roles in cytoplasmic virion envelopment and interactions with
gK. -
pubmed
We propose a method to specifically study the impact of
actomyosin contractility on the intracellular dynamic signal by performing high throughput , comparative measurements of multicellular aggregates with and without
blebbistatin action , a selective inhibitor of
class - II myosins that disrupts
actomyosin contractile activity . -
pubmed
A three - dimensional
hemin - functionalized
graphene hydrogel ( Hem /
GH ) was prepared by a facile self - assembly approach . -
pubmed
The as - prepared Hem /
GH showed good mechanical strength with a storage modulus of 609 - 642 kPa and a high adsorption capacity to organic dye contaminants ( 341 mg g-1 for
rhodamine B ) . -
pubmed
Moreover ,
Hem /
GH could be used as a photosensitizer for the photocatalytic degradation of organic dyes and displayed superior photodegradation activity of
methylene blue (
MB ) . -
pubmed
This result was better than that of counterparts such as
graphene hydrogel (
GH ) and commercial catalyst
P25 . -
pubmed
The excellent cycling performance of the
Hem /
GH was well maintained even after multiple cycles on adsorption process and photocatalytic reaction . -
pubmed
As an extended application , visually intuitive observation showed the as - prepared
Hem /
GH also had strong antibacterial properties . -
pubmed
Cellular mechanisms contributing to the complex machinery executing eryptosis include increase of cytosolic
Ca2 + activity ( [
Ca2+]i ) , oxidative stress ,
ceramide ,
protein kinase C and
caspases . -
pubmed
METHODS :
Phosphatidylserine exposure at the cell surface was estimated from
annexin - V - binding , cell volume from forward scatter , [
Ca2 +]i from
Fluo-3 fluorescence , ROS formation from
DCFDA dependent fluorescence , and
ceramide abundance utilizing specific antibodies . -
pubmed
The effect of
Camalexin on
annexin - V - binding was significantly blunted by removal of extracellular
Ca2 + , by kinase inhibitors
staurosporine ( 1 microM ) and
chelerythrine ( 10 microM ) , as well as by
caspase inhibitors
zVAD ( 10 microM ) and
zIETD - fmk ( 50 microM ) . -
pubmed
CONCLUSIONS :
Camalexin triggers cell shrinkage and phospholipid scrambling of the erythrocyte cell membrane , an effect at least in part depending on
Ca2 + entry , as well as
staurosporine and
chelerythrine sensitive kinase(s ) as well as zVAD and
zIETD - fmk sensitive
caspase ( s ) . -
pubmed
Fifty - six maxillaryanterior - teeth were filled with
TAP for 28 days , and assigned into 5 experimental groups ( n=10 ): Syringe irrigation (
SI ) , NaviTip
FX (
NFX ) , Vibringe - Syringe irrigation ( V -
SI ) , NaviTip
FX - Vibringe ( V -
NFX ) , passive ultrasonic irrigation ( PUI ) and positive and negative control groups ( n=3 for each ) . -
pubmed
V -
NFX and
NFX showed similar performances ( p>0.05 ) while
V - NFX was significantly more efficient than
SI and PUI ( p<0.05 ) at all parts of root canals . -
pubmed
VSI was less efficient at apical part when compared to
NFX and
V - NFX ( p<0.05 ) while not significantly different from PUI and
SI at all parts of the root canals ( p>0.05 ) . -
pubmed
The median sphenoid
sinus mucosal thickness was greater in the
PA than NFPA groups ( 2.0 vs. 0.5 mm ; p & lt ; 0.001 ) . -
pubmed
The small , stable
heme protein
myoglobin (
Mb ) was modified through cofactor substitution and mutagenesis to develop a new catalyst for
carbene transfer reactions . -
pubmed
The native
heme was removed from wild - type Mb and several Mb
His64 mutants ( H64D , H64A , H64V ) , and the resulting
apoproteins were reconstituted with
ruthenium mesoporphyrin IX (
RuMpIX ) . -
pubmed
Sperm counts or motility was significantly reduced by
BPA ,
BPE or
BPS exposure on
PND 60 or
PND 90 . -
pubmed
PCSK9 inhibitors ,
apolipoprotein ( a)-antisense , and
apolipoprotein ( B-100)-antisense
mipomersen have shown promising results . -
pubmed
Over 4 years he had recurrent disease in the
face and liver and was treated with nab -
paclitaxel , surgery , and radioembolization , but continued to have progressive disease . -
pubmed
His
tumor was found to express
PD - L1 and he received off - label
pembrolizumab 2 mg / kg every 21 days for 13 cycles with marked shrinkage of his
liver disease and no new
facial lesions . -
pubmed
The
HCB increased with increasing competitive interactions described by tree - centered competition measure :
BAL or
HCI , and species mixing effects described by BAPOR . -
pubmed
In addition ,
FPHPe1 significantly ( p & lt ; 0.05 ) reduced
SBP ( maximum -33 mmHg ) ,
DBP ( maximum -24 mmHg ) , MAP ( maximum -28 mmHg ) , and HR ( maximum -58 beats ) in SHRs . -
pubmed
BACKGROUND : The
inositol 1,4,5 - triphosphate ( IP3 ) receptor type 1 gene (
ITPR1 ) encodes the
IP3 receptor type 1 (
IP3R1 ) , which modulates intracellular
calcium homeostasis and signaling . -
pubmed
Acetylation of
alpha - tubulin at
Lys-40 is a potential biomarker for
cognitive deficits in various
neurological disorders . -
pubmed
This method has been successfully applied in a preclinical study of
organophosphate neurotoxicity , and we found that chronic exposure to
chlorpyrifos led to a significant and persistent inhibition of brain
tubulin acetylation . -
pubmed
In order to understand water chemistry after impoundment of the Three Gorges Reservoir (
TGR ) , the authors assessed the hydrogeochemical parameters ( water temperature , pH , conductivity , dissolved
O2 ) , major element composition , and the
carbon stable isotopic value of dissolved inorganic
carbon ( delta13CDIC ) in summer and winter at various depths in the water column at
TGR in 2009 . -
pubmed
Ultimately , miR372 promotes the expression of
erbB-2 through
PKM2 - pH3T11 - acetylation on
histone H3 lysine 9 ( H3K9Ac ) pathway . -
pubmed
Fluorescence in situ hybridization demonstrated an
ETV6 - neurotrophic
tyrosine kinase , receptor , type 3 gene (
NTRK3 ) translocation but no
ALK ,
ROS1 , or
PDGFR - beta translocations . -
pubmed
Materials and Methods : Thirty specimens of each denture base material : Heat cure
polymethylmethacrylate ( DPI heat cure ) , high - impact
polymethylmethacrylate ( Trevalon
HI ) , and nylon ( Valplast ) were fabricated . -
pubmed
Voclosporin , a novel
calcineurin inhibitor , has been investigated as induction therapy in LN in combination with
myocophenolate mofetil (
MMF ) and a
glucocorticoid (
GC ) . -
pubmed
Two phase
II trials of
voclosporin were the first trials of a potential treatment of active
LN that met their primary endpoints . -
pubmed
In the phase IIb randomized controlled trial AURA - LV ,
voclosporin was shown to be superior to placebo , when used in combination with
MMF ( 1 - 2 g / day ) and
GC , in achieving remission in active
LN . -
pubmed
Rib Fractures Mimicking Bone Metastases in
18F - PSMA-1007 PET /
CT for
Prostate Cancer . -
pubmed
PET /
CT showed
F - PSMA-1007 uptake in healing
rib fractures with no other pathologic findings . -
pubmed
In this symposium , upcoming challenges of the modern
MS techniques , such as electrospray ionization- and matrix - assisted laser desorption ionization -
MS , in clinical tests for discovering biomarkers are being presented for the rapid diagnoses of
infectious diseases and
amyloidosis , and rapid detections of hormones and
lipids in clinical samples by 4 speakers . -
pubmed
Fragment - based ligand design , De novo design , ADMET and Molecular docking were performed to explore potential selective
SHP2 allosteric inhibitors based on
SHP836 . -
pubmed
Two - pore domain
K + (
K2P ) channels generate K+ leak current , which serves a vital role in controlling and modulating neuronal excitability . -
pubmed
Engineering
transcription factor BmoR for screening
butanol overproducers . -
pubmed
This study took
transcription factor BmoR as an example and aimed to expand the detection range of
BmoR for screening
alcohols overproducers . -
pubmed
Structure of
BmoR -
isobutanol complex were modelled , and
isobutanol binding sites were confirmed by site - directed mutagenesis . -
pubmed
Compared with wild - type
BmoR , F276A / E627N
BmoR and D333N / E627N
BmoR have wider detection ranges ( 0 - 100 mM ) and relatively high outputs to the
isobutanol added quantitatively or produced intracellularly , demonstrating they have potential for screening
isobutanol overproduction
strains . -
pubmed
PAS staining indicated localization of deposits in the sub - retinal
pigment epithelia (
RPE ) layer . -
pubmed
The morphology and textural properties of ILM /
ZIF-8 were characterized by SEM ,
TEM , BET and BJH . -
pubmed
The ILM /
ZIF-8 was applied to the concentration and determination of
aflaoxins (
AFB1 ,
AFB2 ,
AFG1 and
AFG2 ) in milk samples based on magnetic solid phase extraction ( MSPE ) coupled with ultra high performance liquid chromatography - tandem mass spectrometry ( UHPLC -
MS /
MS ) . -
pubmed
ER - mediated anti -
tumor effects of
shikonin on
breast cancer . -
pubmed
This study aims to assess the effects of
shikonin on the cell proliferation , cell cycle and cell apoptosis of
BC and whether the effects are related to
ER /
GPER signaling pathway . -
pubmed
Furthermore ,
shikonin downregulated the expression of
ERalpha and
GPER , and this effect was not affected by the
estrogen environment . -
pubmed
Albumin - stabilized
manganese - based nanocomposites with sensitive
tumor microenvironment responsivity and their application for efficient SiRNA delivery in
brain tumors . -
pubmed
Arg - Gly - Asp ( RGD ) peptide - decorated
bovine serum albumin (
BSA ) was employed as the stabilizer and scaffold to fabricate
Mn ( iii )- and
Mn ( iv )-integrated nanocomposites ( RGD - BMnNPs ) with suitable size , good stability , and excellent biocompatibility . -
pubmed
These novel
albumin - stabilized
manganese - based NPs combined with efficient drug delivery capacity hold great potential to serve as intelligent theranostic agents for further clinical translation . -
pubmed
The asymmetric supercapacitor (
ASC ) device fabricated by employing
Ni -
CoP3 and activated
carbon ( AC ) as positive and negative electrodes , resepectively , exhibits a remarkable high energy density of 89.6 Wh kg-1 at 796 W kg-1 and excellent stability of 93 % after 10 000 cycles , due to the
skutterudite structure . -
pubmed
At 24hr.after surgery , a decrease in
hemoglobin in the
DDAVP group was significantly lower than that in the
NS group ( -5.0 + -6.9 g / L vs. -10.2 + -9.3g / L , p=0.03 ) . -
pubmed
Sonoclot showed that the platelet function in the
DDAVP group was higher than that in
NS group at 24 hr . -
pubmed
Kinetic studies were performed using
4 - nitrophenol ( 4 -
NP ) as the model substrate , using the Langmuir - Hinshelwood model . -
pubmed
CONCLUSIONS : Our results suggest that
SP ,
CGRP and
noradrenaline may promote , while
acetylcholine may stall , the progression of
adenomyosis through their respective receptors on
adenomyotic lesions . -
pubmed
The neoantigen migrated in immunoelectrophoresis as an
alpha2 - beta globulin , had an average molecular size of 7S , and was soluble in 50 % saturated
ammonium sulfate . -
pubmed
Expression of
K-19 was evaluated by immunoperoxidase staining in
formalin - fixed
normal ectocervical tissue ,
normal endocervical tissue ,
cervical dysplasia ,
squamous metaplasia ,
atrophic epithelium ,
cervical condylomas , and invasive
carcinoma to determine if a correlation of
K-19 expression with
dysplasia was present in the cervical epithelium . -
pubmed
Paraffin sections of brain containing the ependymal
wound were reacted for
glial fibrillary acidic protein by the avidin
biotin complex technique . -
pubmed
Non - hydrolysable analogues of
GTP , such as
GTP gamma S and
GMP -
PNP , have previously been shown to inhibit the formation of constitutive secretory vesicles ( CSVs ) and immature secretory granules ( ISGs ) from the trans - Golgi network ( TGN ) . -
pubmed
ADP - ribosylation experiments revealed the presence of a
pertussis toxin - sensitive
G - protein alpha subunit in the TGN . -
pubmed
Several treatments from the latter category , namely ,
hypertensive ,
hypervolemic hemodilutional therapy and the
calcium channel blocker
nimodipine , have proven moderately effective and are in widespread clinical use . -
pubmed
However we are still in need of an effective agent to prevent arterial narrowing , and several hopeful candidates in this category of treatment are clot lytic agent
tissue plasminogen activator (
rt - PA ) and an inhibitor of
iron - dependent peroxidation ,
21 - aminosteroid U74006F (
tirilazad mesylate ) . -
pubmed
The most likely explanation for increased
CSF lactate is
ammonia - induced inhibition of
malate -
aspartate shuttle and/or inhibition of
tricarboxylic acid cycle flux in brain . -
pubmed
The
ras - related
rap2 gene encodes a 21 kDa
GTP - binding protein that exhibits many structural similarities with
Ras proteins . -
pubmed
In particular , it contains a C - terminal CAAX sequence ( C ,
cysteine ; A , aliphatic residue ; X , any
amino acid ) which has been shown to direct the
post - translational modifications responsible for membrane binding of
Ras proteins and
nuclear lamins . -
pubmed
During its maturation , the
Rap2 protein is modified by the attachment of both
palmitate and
polyisoprenoid groups , as is also the case for
H - and N - Ras proteins . -
pubmed
Subcellular fractionation by
sucrose density centrifugation as well as indirect immunofluorescence experiments show that the
Rap2 protein is localized in a low - density compartment that morphologically overlaps with the endoplasmic reticulum , whereas
Ras proteins are associated with the plasma membrane . -
pubmed
17 beta - Bromoacetoxydihydrotesterone (
BA - DHT ) reacted with highly purified
SHBG in a time - dependent and irreversible fashion . -
pubmed
At the completion of the reaction , the molar ratio of
BA - DHT to
SHBG was approximately unity . -
pubmed
SHBG was affinity labeled with
[ 14C ] BA - DHT and submitted to
acid hydrolysis . -
pubmed
Furthermore ,
[ 14C ] BA - DHT - labeled
SHBG was digested with
trypsin , followed by isolation of the released tryptic peptides by reverse - phase high performance liquid chromatography . -
pubmed
It contained 2 '
histidyl residues , corresponding to residues 235 and 251 in the known
amino acid sequence of
SHBG . -
pubmed
Cancer cells were equally stained with fluorescent
R 1881 and
mibolerone , and this fluorescence seems to be made up of both the
androgen receptor and
progestin - binding protein . -
pubmed
The immunoreactivities of these
McAbs to 33 KD appear to be directed to peptide epitopes but not to
carbohydrate moiety . -
pubmed
An increase in the [
Ca2+]o from 0.45 to 3.6 mM depressed both the spontaneous and the
nicotine - induced release of
ACh , while the releases of
ACh induced by electrical - field stimulation (
EFS ) or by high
K + were augmented . -
pubmed
IND and
PGE2 did not modify the release of
ACh induced by
EFS and high
K + , at any given [
Ca2+]o tested . -
pubmed
Cyclic AMP perturbation of intact cells caused induction of both
phosphorylase -' a ' activity and the 104 - kDa protein . -
pubmed
Although a causative contribution of endogenous
ANF in the natriuretic response to acute
hypervolemia is well established , its participation in the maintenance of salt balance during large variations in dietary
Na intake is less certain . -
pubmed
In addition to increased
sodium excretion , atrial factor also inhibits
aldosterone and
renin release , both in vitro and in vivo . -
pubmed
the change in
pulse interval in response to a
phenylephrine - induced
increase in blood pressure , was compared with ( 1 ) haemodynamic changes during mental arithmetic , a reaction time test , isometric and bicycle exercise ; ( 2 ) plasma
noradrenaline ( PNA ) concentrations at
rest , and during bicycle exercise and ; ( 3 ) the variability of ambulatory intra - arterial blood pressure . -
pubmed
A specific anti -
NAS antibody and
fluorescein - labelled second antibody were employed to investigate the presence of NAS in the dentate gyrus of the hippocampus as a function of age . -
pubmed
The
enzyme is free of
lipase activity , and possesses a very high sterospecificity on
S - naproxen [
2 -( 6 - methoxy-2 - naphthyl )- propionic acid ] esters and
esters of related drugs . -
pubmed
The primary structure of the
enzyme , determined from the
nucleotide sequence , shows limited homology with the catalytic site of
lipases . -
pubmed
To increase the resistance of the
enzyme ,
lysine residues thought to be responsible for this phenomnon were replaced through site - directed mutagenesis . -
pubmed
Potentiation by
ACE inhibitors of the dilator response to
bradykinin in the coronary microcirculation : interaction at the receptor level . -
pubmed
Continuous infusion of
bradykinin ( 10 nM ) elicited a transient decrease in coronary perfusion pressure (
CPP ) and increased
prostacyclin (
PGI2 ) release which returned to baseline values within 30 min . -
pubmed
Subsequent co - infusion of
ramiprilat ( & gt ; or = 10 nM ) or
moexiprilat , but not of the less potent
ACE inhibitor
n - octyl - ramipril (
RA - octyl ) , caused another fall in
CPP and an increase in
PGI2 release , the magnitude and time course of which were almost identical to the first response to
bradykinin . -
pubmed
No change in
CPP or
PGI2 release was observed when the
ACE inhibitors were administered in the absence of exogenous
bradykinin . -
pubmed
Infusion of
D - Arg [ Hyp3 ]- bradykinin ( 10 nM ) , a specific
B2 - receptor agonist which was significantly more resistant to degradation by
ACE than
bradykinin , produced virtually identical changes in
CPP and
PGI2 release when compared to
bradykinin . -
pubmed
Subsequent co - infusion of
ramiprilat was similarly effective in restoring the fall in
CPP and increase in
PGI2 release elicited by
D - Arg [ Hyp3 ]- bradykinin as in the presence of
bradykinin . -
pubmed
In concentrations which should block the degradation of
bradykinin by
ACE in the coronary vascular bed , two
ACE substrates ,
hippuryl - L - histidyl - L - leucine ( 0.2 mM ) and
angiotensin I ( 0.3 microM ) , were unable to elicit a significant change in
CPP or
PGI2 release while
ramiprilat and another
ACE inhibitor ,
quinaprilat , were still active in the presence of these substrates . -
pubmed
Ramiprilat ( 0.1 MicroM ) , but not
RA - octyl ( 1 MicroM),potentiated the endothelium - independent ,
B2 - receptor - mediated constrictor response to
bradykinin , but not that to the
thromboxane - mimetic
U46619 (
9,11 - dideoxy - ll alpha,9 alpha - epoxymethano - prostaglandin F2.).Moreover ,
ramiprilat but not
RA - octyl caused a concentration - dependent ,
B2 - receptor antagonist sensitive increase in tone when administered alone.7 . -
pubmed
Bilateral simultaneous inferior petrosal
sinus corticotropin sampling with
corticotropin - releasing hormone stimulation was performed before transphenoidal pituitary adenomectomy , with successful localization of the
pituitary adenoma . -
pubmed
The test relies on the capacity of the kidney to retain
H + during gastric acid secretion , leading to a
post - prandial urine ' alkaline tide ' . -
pubmed
Different qualitative patterns are seen when the activities of
alkaline phosphatase (
AP ) ,
invertase (
INV ) , or
[ 3H ] methylthymidine incorporation are expressed on the basis of millimoles per gram protein , percentage of highest activity , percentage of total activity , cumulative activity for each fraction , or millimoles per gram protein versus cumulative percentage of
total protein . -
pubmed
To identify gene products that interact with
Notch , a second - site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated
Notch molecules . -
pubmed
Metallothioneins (
MTs ) are low molecular weight and
cysteine - rich intracellular proteins involved in metal homeostasis and detoxication . -
pubmed
nidulans imply a role for
topoisomerases in amplification and replication of circular DNA
molecules . -
pubmed
Tubulin is a very
water soluble protein , yet a significant portion is firmly associated with cell membranes . -
pubmed
More
palmitate of the pellet was found in alpha than
beta tubulin ;
EGF stimulation primarily increased
palmitate in
beta tubulin . -
pubmed
In addition , blood samples were taken at
rest and at steady state during exercise , and plasma levels of
vasopressin and
renin activity were assessed . -
pubmed
At
rest and during all levels of exercise ,
muscarinic cholinergic receptor blockade caused a marked increase in HR over control ( saline treated ) levels with little effect on MAP or
TAQ . -
pubmed
Summary of the nephron distribution of
PDE activities showed that
cAMP - PDE activities were detected in all nephron segments and the distal convoluted tubules ( DCTs ) had the highest activity . -
pubmed
Both in proximal convoluted tubules ( PCTs ) and medullary collecting ducts ( MCDs ) , more than 80 % of the total
cAMP - PDE activities were inhibited by a nonspecific
PDE inhibitor ,
3 - isobutyl-1 - methylxanthine (
IBMX ) . -
pubmed
In PCTs ,
rolipram (
type - IV PDE inhibitor ) was an equally potent inhibitor of
IBMX , while
8 - methoxymethyl - IBMX (
MM - IBMX ,
type - I PDE inhibitor ) and
cilostamide (
type - III PDE inhibitor ) inhibited
cAMP - PDE by 35 and 57 % , respectively . -
pubmed
Rolipram potentiated the
parathyroid hormone (
PTH)-induced
cAMP content in PCTs most effectively . -
pubmed
Cilostamide had no effect on the
cAMP content stimulated by
PTH or
AVP in PCTs and
MCDs . -
pubmed
Among these ,
type - IV PDE was responsible for hydrolysis of
cAMP in PCTs , and
type - I and IV PDE were responsible for that in
MCDs . -
pubmed
Nine chlorinated metabolites produced from
DDE were identified by gas chromatography - mass spectrometry - Fourier - transform infrared spectrometry (
GC -
MS - FTIR ) from cultures grown on
biphenyl . -
pubmed
Role of quaternary structure in
muscle creatine kinase stability :
tryptophan 210 is important for dimer cohesion . -
pubmed
A mutant of the dimeric
rabbit muscle creatine kinase (
MM - CK ) in which
tryptophan 210 was replaced has been studied to assess the role of this residue in dimer cohesion and the importance of the dimeric state for the native enzyme stability . -
pubmed
All mutants exhibited wild - type preference for large hydrophobic residues , especially
Phe , in the
P1 ' substrate position . -
pubmed
These
amino acid positions are structurally equivalent to
Arg8 ,
Leu10 ,
Val32 ,
Met46 ,
Ile54 , and
Ile84 in HIV-1
PR , respectively , which mutate in drug - resistance . -
pubmed
with the
glucose antimetabolite ,
2 - deoxy - D - glucose (
2DG : 100 or 400 mg / kg ) , or the vehicle , saline , prior to onset of the expected
LH surge . -
pubmed
In other studies , i.c.v delivery of the
opioid receptor antagonist ,
naltrexone (
NALT ) , partially reversed the inhibitory effects of
2DG on the gonadal
steroid - induced
LH surge . -
pubmed
These studies show that magnitude of the
LH surge is decreased by
glucose substrate imbalance , and that regulatory effects of this metabolic challenge on the reproductive neuroendocrine axis is correlated with alterations in the transcriptional activation of preoptic
GnRH neurones by gonadal
steroid positive feedback . -
pubmed
BM 144 : an original
thromboxane A2 receptor antagonist derived from
torasemide . -
pubmed
With the aim to develop more potent
TXA2 receptor antagonists , we investigated a series of
torasemide derivatives . -
pubmed
This pharmacomodulation led to the discovery of a
sulfonyl - cyanoguanidine (
BM 144 ) which presents the same pharmacological profile as
sulotroban , a
TXA2 receptor antagonist used as reference . -
pubmed
The
retinoid concentrations returned to normal within 1 - 2 days
post - irradiation , despite there being no overt increase in the uptake of
[ 3H ] retinol or the biosynthesis of
3,4 - didehydroretinol . -
pubmed
It has been known for many years that the absence of
post - transcriptional modification at A1519 and the adjacent A1518 in
strains lacking a functional
KsgA methylase produces a
kasugamycin resistance phenotype . -
pubmed
Residues A794 and G926 are protected from chemical modification by
kasugamycin and
tRNA , and have been implicated in the initiation of protein synthesis . -
pubmed
Post - natal modulation of heart and liver
phosphoglyceride fatty acids in pups . -
pubmed
Changes in
fatty acid composition specific to tissue ( heart and liver ) and
phosphoglyceride (
CPG and
EPG ) occurred
post - natally . -
pubmed
With the exception in the heart
EPG , the levels of
arachidonic acid did not decrease concomitantly . -
pubmed
Although the
fatty acid composition of the diet did not change between pregnancy and lactation ,
docosahexaenoic and total n-3 increased in heart CPG and
EPG and liver CPG of the suckling pups . -
pubmed
The
isoflavone genistein may either stimulate or inhibit
cystic fibrosis transmembrane conductance regulator (
CFTR ) Cl- channels . -
pubmed
Single - channel analysis of channel block suggested that
genistein ( 100 microM ) may inhibit
CFTR by two mechanisms : first , it may slow the rate of channel opening and second , it may block open channels . -
pubmed
Acidification of the intracellular solution relieved channel block , suggesting that the anionic form of
genistein may inhibit
CFTR . -
pubmed
Genistein inhibition of
CFTR Cl- currents was weakly voltage dependent and unaffected by changes in the extracellular
Cl- concentration . -
pubmed
Channel block was relieved by
pyrophosphate ( 5 mM ) and
ATP ( 5 mM ) , two agents that interact with the
nucleotide - binding domains ( NBDs ) of
CFTR to greatly stimulate channel activity . -
pubmed
The data suggest that
genistein may inhibit
CFTR by two mechanisms . -
pubmed
Second , it may bind within the
CFTR pore to weakly block
Cl- permeation . -
pubmed
We proved a
GC -
MS - method with
d ( 3 )- morphine as internal standard and silylation with
MSTFA . -
pubmed
Biologic effects included induction of low plasma concentrations of
tumor necrosis factor - alpha and
interleukin-6 observed immediately after
MDXH210 infusion and 70 % saturation of circulating monocyte - associated
Fc gamma RI with
MDXH210 at a dose level of 4 to 8 mg / m2 . -
pubmed
Quantitative determination of phospholipid compositions by ESI -
MS : effects of
acyl chain length , unsaturation , and lipid concentration on instrument response . -
pubmed
DNA -
XPA interactions : a
( 31 ) P NMR and molecular modeling study of dCCAATAACC association with the minimal DNA - binding domain ( M98 - F219 ) of the
nucleotide excision repair protein
XPA . -
pubmed
Here ,
XPA - DNA interactions are further characterized by NMR spectroscopy from the vantage point of the DNA using a single - stranded DNA nonamer ,
dCCAATAACC ( d9 ) . -
pubmed
A subset of
( 31 ) P resonances of d9 were observed to broaden and/or shift providing direct evidence that
XPA -
MBD binds d9 by a mechanism that perturbs the
phosphodiester backbone of d9 . -
pubmed
The interior five residues of d9 broadened and/or shifted before
( 31 ) P resonances of
phosphate groups at the termini , suggesting that when d9 is bound to
XPA - MBD the internal residues assume a correlation time that is characteristic of the molecular weight of the complex while the residues at the termini undergo a fraying motion away from the surface of the
protein on a timescale such that the line widths are more characteristic of the molecular weight of ssDNA . -
pubmed
A molecular model of the
XPA - MBD complex with d9 was calculated based on the
( 15 ) N (
XPA - MBD ) and
( 31 ) P ( d9 ) chemical shift mapping studies and on the assumption that electrostatic interactions drive the complex formation . -
pubmed
Even though the 4 - week and 8 - week exercise training caused 53 and 25 % increases , respectively , in total
cytochrome P450 contents in the liver , exercise training did not cause any changes in the levels of
P450 1A2 ( which primarily metabolizes
azosemide ) , 2E1 and 3A23 in the liver , as assessed by both Western and Northern blot analyses . -
pubmed
UNLABELLED : The standard
celite or kaolin activated clotting time (
ACT ) correlates poorly with
heparin levels during cardiopulmonary bypass (
CPB ) . -
pubmed
We compared a modified kaolin
ACT , in which plasma was supplemented , to a standard undiluted kaolin
ACT for monitoring
heparin levels during
CPB . -
pubmed
Heparin management was performed according to the Hepcon HMS results ( Medtronic , Minneapolis ,
MN ) . -
pubmed
The data obtained were correlated to the plasma chromogenic
anti - Xa activity as a reference assay for
heparin levels . -
pubmed
Methylation of
lysine-79 (
K79 ) within the globular domain of
histone H3 by
Dot1 methylase is important for transcriptional silencing and for association of the
Sir silencing proteins in yeast . -
pubmed
Among these , the
terminal transferase - mediated
dUTP nick end - labeling ( TUNEL ) assay remains the most widely used technique . -
pubmed
We developed a technique consisting of staining live cells and tissues with
Hoechst 33342 and the vital dye
propidium iodide (
PI ) , followed by fixation and the TUNEL reaction . -
pubmed
Cusp - shaped conductivity spectra , consistent with weak impurity scattering of
nodal quasiparticles , were obtained in the 0.6 - 21 GHz frequency range in highly ordered crystals of
YBa2Cu3O6.50 and
YBa2Cu3O6.99 . -
pubmed
Eukaryotic initiation factor 5A (
eIF-5A ) contains an unusual
amino acid ,
hypusine , which is formed
post - translationally . -
pubmed
Although
eIF-5A and its
hypusine modification are essential for eukaryotic cell viability , the precise physiological function of it has remained elusive . -
pubmed
It is concluded that
hypusine formation of
eIF-5A exits in the regulation of cell cycle and the results suggest that
eIF-5A is involved in the expression of proteins regulating transition of G(1)-S phase of cell cycle . -
pubmed
[ USE OF
CHLORDIAZEPOXIDE ( BALANCE )
AT UROLOGICAL CLINIC ] . -
pubmed
Ganglioside GM3 blocks the activation of
epidermal growth factor receptor induced by
integrin at specific
tyrosine sites . -
pubmed
Previous studies have shown that ligand - dependent
EGFR activation is inhibited by GM3 , the predominant
ganglioside of epithelial cells , but the effect of GM3 on ligand - independent ,
integrin -
EGFR cross - talk is unknown . -
pubmed
Consistently , endogenous depletion of GM3 markedly increases the association of
EGFR with
tyrosine - phosphorylated
integrin beta1 and promotes cell proliferation . -
pubmed
Stimulation of
EGFR and
mitogen - activated protein kinase signaling by GM3 depletion involves the phosphorylation of
EGFR at
tyrosine residues 845 , 1068 , and 1148 but not 1086 or 1173 . -
pubmed
The specific blockade of phosphorylation at
Tyr-845 with
Src family kinase inhibition and at
Tyr-1148 with
phosphatidylinositol 3 - kinase inhibition suggests that
GM3 inhibits
integrin - induced , ligand - independent
EGFR phosphorylation ( cross - talk ) through suppression of
Src family kinase and
phosphatidylinositol 3 - kinase signaling . -
pubmed
Taking 10(5 ) cells as a basis for comparisons , HAFs on
PUF /
SF exhibited initially higher
glucose consumption rates , but persistently lower
glutamine uptake rates than on
PUF , whereas the rates of
lactate and
interleukin 6 release and of extracellular assembly of
type I collagen fibers were alike on either substrate . -
pubmed
This entry was attenuated by
2 - aminoethoxydiphenylborane ,
L - type Ca ( 2 + ) channel blockade ,
pinacidil , and
Gd ( 3 + ) . -
pubmed
The CCE - evoked contraction was sensitive to
2 - aminoethoxydiphenylborane ,
L - type Ca ( 2 + ) channel blockers , and
Gd ( 3 + ) . -
pubmed
E2 and
E2 + P4 groups learned in fewer trials , and performed better in the radial maze and the working memory task than the other groups under the normal -
calcium condition . -
pubmed
In vitro treatment of embryonic pancreata with
dexamethasone , a
glucocorticoid agonist , induced a decrease of
insulin - expressing cell numbers and a doubling of acinar cell area , indicating that glucocorticoids favored acinar differentiation ; in line with this , expression of
Pdx-1 ,
Pax-6 , and
Nkx6.1 was downregulated , whereas the mRNA levels of
Ptf1 -
p48 and
Hes-1 were increased . -
pubmed
We developed a diagnostic array of
oligonucleotide probes targeting species - specific variable regions of the genes encoding topoisomerases
GyrB and
ParE of respiratory bacterial pathogens . -
pubmed
Both short- and long - term
alcohol exposure also affect the
serotonin receptors that convert the chemical signal produced by
serotonin into functional changes in the signal - receiving cell . -
pubmed
Pretreatment with
protein kinase C (
PKC ) inhibitor blocked the
TPA - induced increase in
NAG-1 protein levels and
NF - kappa B binding / transcriptional activity , whereas an inhibition of
p38 ,
JNK ,
MEK activity had no effect on
TPA - induced
NAG-1 levels and
NF - kappa B transcriptional activity . -
pubmed
A single round of random mutagenesis followed by screening for hydrolytic activity for
oleoyl 2 - naphthylamide as compared with that for
oleoyl 2 - naphthyl ester identified five mutants with 1.7 - 2.0 - fold increased relative
amidase activities . -
pubmed
Active - site titration using a fluorescent
phosphonic acid ester allowed the molecular activities for the
amide and the
ester to be determined for each mutant without purification of the
lipase . -
pubmed
A double mutant F207S / A213D gave the highest molecular activity of 1.1 min(-1 ) for the
amide , corresponding to a 2 - fold increase compared with that of the wild - type
lipase . -
pubmed
A structural model of the
lipase indicated that the mutations occurred at the sites near the surface and remote from the catalytic
triad , but close to the
calcium binding site . -
pubmed
This study is a first step towards understanding why
lipases do not hydrolyze
amides despite the similarities to
serine proteases in the active site structure and the reaction mechanism and towards the preparation of a general
acyl transfer catalyst for the biotransformation of
amides . -
pubmed
Fluorescence quantum yield and lifetime of
APL have been measured to be lower than those of the bare fluorophore ( AP ) due to the photoinduced intramolecular electron transfer ( PIET ) between the fluorophore and the
receptor moieties of the molecule . -
pubmed
Emerging evidence supports the recognition and management of secondary lipid goals ,
high - density lipoprotein cholesterol (
HDL - C ) level greater than 40 mg / dL , and
triglyceride level less than 150 mg / dL. -
pubmed
Preparation of the whole brain sample incorporated a highly efficient
cysteinyl - peptide enrichment (
CPE ) technique to complement a global enzymatic digestion method . -
pubmed
Both the global and the
cysteinyl - enriched peptide samples were analyzed by
SCX fractionation coupled with reversed phase LC -
MS /
MS analysis . -
pubmed
RESULTS : By stepwise multivariate analyses , hemodynamic parameters such as left ventricular ejection fraction ( LVEF ) and left ventricular end -
diastolic pressure ( LVEDP ) but not
eGFR were independent predictors of a transcardiac increase ( coronary
sinus - aortic root ) in
BNP . -
pubmed
Subtype specificity of scorpion beta - toxin
Tz1 interaction with voltage - gated
sodium channels is determined by the pore loop of domain 3 . -
pubmed
A 22 - gauge needle was advanced to the sympathetic trunk at midlumbar level with
CT fluoroscopic guidance , and 1 mL of
iopamidol ( 200 mg of
iodine per milliliter ) and 5 mL of 0.5 %
bupivacaine were injected . -
pubmed
Needle tip position and distribution of
bupivacaine were measured on
CT images . -
pubmed
Genes of a
glutathione S - transferase and a
cytochrome P450 , which were induced by
2,4,6 - trinitrotoluene exposure in previous studies , were upregulated by 10- and 8 - fold , respectively . -
pubmed
pneumophila secretes a siderophore (
legiobactin ) that is detectable by the
CAS assay . -
pubmed
The
lbtA mutant is also defective for growth in deferrated media containing
citrate , indicating that
legiobactin is required in conditions of severe
iron limitation . -
pubmed
pneumophila does express
lbtA in macrophages , suggesting that
legiobactin has a dispensable role in
infection . -
pubmed
Anion - exchange HPLC has been used to purify
legiobactin , and thus far , structural analysis suggests that the molecule is similar but not identical to
rhizobactin ,
rhizoferrin , and
alcaligin . -
pubmed
pneumophila
cytochrome c maturation locus in low -
iron growth ,
intracellular infection , and virulence . -
pubmed
STUDY DESIGN : Retrospective observational analysis of claims - based data of health plan members with
CAD receiving 1 or more prescriptions per year of any of the following classes of medications used for secondary prevention of
CAD : lipid - lowering agents ,
angiotensin - converting enzyme ( ACE ) inhibitors , or
angiotensin receptor blockers ( ARBs ) and beta - blockers . -
pubmed
Thus ,
CD4 T cells from
IPF patients have characteristics typical of cell - mediated pathologic responses , including augmented effector functions , provision of facultative help for autoantibody production , oligoclonal expansions , and proliferations driven by an
Ag present in diseased tissues . -
pubmed
AIM : To evaluate promotive effect of
hyperthermia on the carcinostatic activity of synthesized
omega - hydroxy fatty acids (
omega HFAs ) and their
ethylesters agaist Ehrlich ascites
tumor ( EAT ) cells . -
pubmed
METHODS : EAT cells were cultured with either
omegaHFAs or their
ethylester derivatives in a water bath at either 37 degrees C or 42 degrees C for 30 min , followed by incubation in a
CO2 incubator for 20 or 72 h.
Mitochond - rial dehydrogenase - based WST-1 assay and
trypan blue dye exclusion assay were then conducted after incubation . -
pubmed
An autoimmune process was considered to be responsible for the repeated episodes of
encephalitis because the symptoms were highly responsive to
steroids and anti -
glutamate receptor antibodies were detected in the
CSF . -
pubmed
Thiopurine - methyltransferase and
inosine triphosphate pyrophosphatase polymorphism in a liver transplant recipient developing nodular regenerative
hyperplasia on low - dose
azathioprine . -
pubmed
The enzymes
thiopurine - methyltransferase (
TPMT ) and
inosine triphosphate pyrophosphatase (
ITPA ) are involved in
thiopurine metabolism . -
pubmed
Geologic estimates suggest that atmospheric
oxygen in the late Paleozoic
Era was 35 % . -
pubmed
The concentration of
GABA is modulated by the
pyridoxal-5 '- phosphate (
PLP)-dependent enzymes ,
GAD and
GABA - T. In this study , we generated
pyridoxyl - gamma - aminobutyrate (
PL - GABA ) , a novel
GABA analogue composed of
pyridoxyl and
GABA , and have also characterized its anticonvulsant and pharmacological functions in vitro . -
pubmed
The results of biodistribution studies revealed that
PL -
GABA is capable of crossing the blood - brain barrier . -
pubmed
PL -
GABA evidenced anticonvulsant activity in a wide range of
epilepsy models , some of which were electrically - based (
MES seizures ) and some chemically - based (
bicuculline ,
pentylenetetrazol (
PTZ ) ,
picrotoxine ,
3 - mercaptopropionic acid ) . -
pubmed
In addition ,
PL -
GABA displayed no signs of tolerance after subchronic ( 10 day ) treatment . -
pubmed
Therefore , our results indicate that PL -
GABA exerts a broad - spectrum anticonvulsant effect , and identify the potential for reduced
PL -
GABA tolerance as an additional positive profile for novel antiepileptic drugs . -
pubmed
Hemoglobin - based
oxygen carriers may play a future role as intravascular volume expanders in lieu of transfusion of red blood cell concentrates . -
pubmed
The recovery of
Amberlite XAD-16 purified
FA was up to 57.97 % with HPLC purity 50.89 % . -
pubmed
The role of the
heterocycle in
bis ( hydroxyphenyl ) triazoles for inhibition of
17beta - Hydroxysteroid Dehydrogenase (
17beta - HSD ) type 1 and type 2 . -
pubmed
17beta - Hydroxysteroid dehydrogenase type 1 (
17beta - HSD1 ) is responsible for the catalytic reduction of the weak
estrogen estrone (
E1 ) into the highly potent
17beta - estradiol (
E2 ) . -
pubmed
As
17beta - HSD1 is often overexpressed in
mammary tumors and
endometriosis , the selective inhibition of this enzyme is discussed as a promising approach for the treatment of
estrogen - dependent diseases . -
pubmed
Recently , we reported on
bis ( hydroxyphenyl ) azoles as a new class of potent inhibitors of
17beta - HSD1 . -
pubmed
Substituents were introduced on the
triazole core in order to establish additional interactions with the
enzyme active site . -
pubmed
3 -[ 4 -( 4 - Hydroxyphenyl )- 1H-1,2,3 - triazol-1 - yl ] phenol ( 3 ) was the most active compound discovered in this study with an IC(50 ) value of 840nM and a reasonable selectivity towards
17beta - HSD2 . -
pubmed
CONCLUSIONS : These data show that dual gene therapy with
ER /
GR might be a useful tool for understanding the role of
steroid hormones in the storage of
traumatic memories . -
pubmed
Huntington ' s disease is a
neurodegenerative disorder caused by a
polyglutamine expansion within the
huntingtin protein . -
pubmed
Blood
glucose and
insulin levels and various appetite hormone concentrations were measured during the study . -
pubmed
Bone metabolism was evaluated by serum
calcium ,
phosphorus ,
alkaline phosphatase ,
parathyroid hormone ,
25 ( OH ) vitamin D ,
osteocalcin ,
serum C - telopeptide cross - linked
collagen type I levels . -
pubmed
Validation of the mechanism of
cholesterol binding by
StAR using short molecular dynamics simulations . -
pubmed
We previously proposed an original two - state
cholesterol binding mechanism by
StAR , in which the C - terminal alpha - helix of
StAR gates the access of
cholesterol to its binding site cavity . -
pubmed
In our molecular model of the
cholesterol -
StAR complex , the hydrophobic moiety of
cholesterol interacts with hydrophobic
amino acid side - chains located in the C - terminal alpha - helix and at the bottom of the cavity . -
pubmed
Molecular dynamics simulations showed that point mutations of
Phe(267 ) ,
Leu(271 ) or
Leu(275 ) at the alpha - helix 4 increased the gyration radius ( more flexibility ) of the
protein 's structure , whereas the salt bridge double mutant E169M / R188 M showed a decrease in flexibility ( more compactness ) . -
pubmed
Endothelial dysfunction is intricately related to
insulin resistance through the parallel stimulatory effects of
insulin on
glucose disposal in metabolic tissues and
NO production in the endothelium . -
pubmed
In all cases , the
prolines pucker substantially and the pucker is associated with the backbone rotation toward the
E2 /
ubiquitin . -
pubmed
In contrast ,
NALP3 functionality in intermediate stage
melanoma cells requires activation of the
IL-1 receptor to secrete active
IL-1beta ; cells from an early stage of
melanoma require stimulation of the
IL-1 receptor plus the co - stimulant
muramyl dipeptide . -
pubmed
Discovery of
7 -( 4 -( 3 - ethynylphenylamino )- 7 - methoxyquinazolin-6 - yloxy )- N - hydroxyheptanamide (
CUDc-101 ) as a potent multi - acting
HDAC ,
EGFR , and
HER2 inhibitor for the treatment of
cancer . -
pubmed
By incorporating
histone deacetylase (
HDAC ) inhibitory functionality into the pharmacophore of the
epidermal growth factor receptor (
EGFR ) and
human epidermal growth factor receptor 2 (
HER2 ) inhibitors , we synthesized a novel series of compounds with potent , multiacting
HDAC ,
EGFR , and
HER2 inhibition and identified
7 -( 4 -( 3 - ethynylphenylamino )- 7 - methoxyquinazolin-6 - yloxy )- N - hydroxyheptanamide 8 (
CUDC-101 ) as a drug candidate , which is now in clinical development . -
pubmed
Uncontrolled studies suggest that
prostacyclin analogues and
phosphodiesterase type-5 inhibitors may have benefits in advanced
pulmonary vascular disease . -
pubmed
Using a BGE of 100 mM
acetic acid ( pH 3.1 ) and a positively charged capillary coating , a highly reproducible and efficient separation of four model proteins (
insulin ,
carbonic anhydrase II ,
ribonuclease A and
lysozyme ) was obtained . -
pubmed
Sheath - liquid CE -
MS using the same porous tip capillary and an
isopropanol - water -
acetic acid sheath liquid showed slightly lower to similar analyte responses . -
pubmed
The analyte response in sheathless CE -
MS could be enhanced using a nanoESI source and adding 5 %
isopropanol to the BGE , leading to improved detection limits by 50 - fold to 140 - fold as compared to sheath liquid interfacing using the same capillary - equivalent to sub - nM detection limits for three out of four proteins . -
pubmed
Sauchinone protects pancreatic beta cells against
cytokine - mediated toxicity . -
pubmed
In this study , we investigated the effect of
sauchinone on
IL-1beta ( 5 ng / ml ) and
IFN - gamma ( 100 U / ml)-induced beta - cell damage . -
pubmed
Sauchinone prevented
nitric oxide (
NO ) production , and this effect was correlated with reduced levels of protein expression of the inducible form of
NO synthase (
iNOS ) . -
pubmed
The molecular mechanism by which
sauchinone inhibits
iNOS gene expression appeared to involve the inhibition of
NF - kappaB activation . -
pubmed
Moreover , pancreatic beta - cells treated with
cytokines upregulated the phosphorylation of
STAT-1 ,
STAT-3 and
STAT-5 , however , pre - treatment with
sauchinone attenuated these effects . -
pubmed
Sauchinone prevented
cytokine - induced
NO production ,
iNOS expression ,
JAK /
STAT activation , and
NF - kappaB activation and inhibition of
glucose - stimulated
insulin secretion ( GSIS ) . -
pubmed
Even though , it has been shown that
TiO ( 2 ) NPs exposure can induce some alterations in mitochondria including
cytochrome c release to cytosol , change in mitochondrial permeability and decrease of mitochondrial membrane potential ( DeltaPsi(m ) ) , there is no information about the changes in mitochondrial function induced by
TiO ( 2 ) NPs . -
pubmed
[ Effect of
vitamin C on the condition of
NO - synthase system in experimental
stomach ulcer ] . -
pubmed
Vit C displayed a pronounced antioxidant action , reduced the degree of destructive affections , diminished the activity of
iNOS and lipoperoxidation processes , decreased the
NO content and
SOD activity . -
pubmed
Combined action of
Vit C with
L - arginine reduced the degree of
GM lesions , activity of
eNOS and the content of
NO in
GM whereas the concentration of
L - arginine in blood was increased . -
pubmed
Under conditions of
Vit C action and
iNOS and
COX-2 blockage , the activity of
NO - synthases and lipoperoxidation processes were slightly decreased , indicating on dominant action of
Vit C. -
pubmed
Here , we describe an engineered split - adaptor system , in which adaptor assembly and delivery of substrates to the
ClpXP protease depends on a small molecule (
rapamycin ) . -
pubmed
Seasonal patterns of
prolactin and
corticosterone secretion in an Antarctic seabird that moults during reproduction . -
pubmed
In avian species that have evolved life - history strategies wherein molt and breeding overlap , there are potential conflicts between the regulatory roles of baseline
prolactin and
corticosterone in parental care ( positive ) and moult ( negative ) . -
pubmed
When comparing
prolactin ,
corticosterone , and moult in failed vs. active breeders , we found that failed breeding enabled a more rapid down - regulation of
prolactin , thus facilitating a more rapid moult . -
pubmed
Enhancing microRNA transfection to inhibit
survivin gene expression and induce apoptosis : could it be mediated by a novel combination of sonoporation and
polyethylenimine ? -
pubmed
The
AT - rich interactive domain 1A gene (
ARID1A ) , which encodes one of the subunits in the Switch /
Sucrose Nonfermentable chromatin remodeling complex , carries mutations and is responsible for loss of protein expression in gastric
carcinoma , particularly with
Epstein - Barr virus ( EBV ) infection and a
microsatellite instability - high phenotype . -
pubmed
OBJECTIVE : The purpose of our study was to prospectively and directly compare capability of
O2 - enhanced MRI , MDCT , and perfusion SPECT /
CT to clinical outcome measurements in candidates for lung volume reduction surgery ( LVRS ) . -
pubmed
Quantitatively assessed upper / lower lung ratios on
O2 - enhanced MRI , MDCT , and SPECT /
CT were calculated from regional relative enhancement ratios , functional lung volumes , and radioisotope uptakes between upper and lower lungs . -
pubmed
Qualitatively assessed upper / lower lung ratios on
O2 - enhanced MRI , MDCT , and SPECT /
CT were estimated using visual scoring systems . -
pubmed
CONCLUSION :
O2 - enhanced MRI shows potential for more accurate evaluation of postoperative clinical outcome for LVRS candidates than SPECT /
CT and can be considered at least as reliable as MDCT . -
pubmed
IMPACT : Adequate
folate status may protect against colorectal
carcinogenesis through mechanisms involving adequate DNA methylation in the genome . -
pubmed
Blood samples were also collected for determining
FBS , HbA1c ,
cholesterol and
triglyceride . -
pubmed
Blood lead ,
delta - aminolevulinic acid dehydratase (
delta - ALAD ) and
zinc protoporphyrin (
ZPP ) concentrations are widely used as biomarkers for
lead toxicity The present study was designed to determine the impact of chronic lead exposure on selected biological markers . -
pubmed
Reflectance difference spectroscopy (
RDS ) is applied to follow in situ the preparation of clean and carburized
W ( 1 1 0 ) surfaces and to study the temperature - induced transition between the R(15 x 3 ) and R(15 x 12 )
carbon /
tungsten surface phases . -
pubmed
alpha - Lipoic acid ameliorates
mitochondrial impairment and reverses apoptosis in
FABP3 - overexpressing embryonic
cancer cells . -
pubmed
Fatty acid - binding protein 3 (
FABP3 ) is a low molecular weight protein with distinct tissue distribution , which may play an important role in
fatty acid transport , cell growth , cellular signaling , and gene transcription . -
pubmed
Furthermore , the
MMP , intracellular
ATP synthesis and the amount of mitochondrial DNA were also increased . -
pubmed
Collectively , our results indicate that abnormalities in
FABP3 expression contribute to
mitochondrial dysfunction and apoptosis , and that
alpha - LA represents a suitable candidate for development as a treatment for apoptosis - related
congenital cardiac malformations . -
pubmed
Calprotectin (
S100A8 / A9 ) ,
S100A12 , and
EDTA - resistant
S100A12 complexes ( ERAC ) in primary
Sjogren ' s syndrome . -
pubmed
Injury - mediated increased
K (+ ) efflux through
Kv2.1 channels promotes neuronal apoptosis , contributing to widespread neuronal loss in
neurodegenerative disorders such as
Alzheimer ' s disease and
stroke . -
pubmed
Diabetic patients have characteristic pattern of
dyslipidemia with decreased level of
high density lipoprotein cholesterol (
HDL - C ) and elevated
triglycerides (
TG ) level . -
pubmed
However , in
diabetes mellitus ,
low density lipoprotein cholesterol (
LDL - C ) which is used as one of the markers for the risk of
CVD , is underestimated so in such cases the levels of non - High density lipoprotein
cholesterol ( non -
HDL - C ) can be a stronger predictor of
CVD as it strongly correlates with atherogenic lipoproteins . -
pubmed
Diabetics have significantly elevated ratio of total
cholesterol to
high density lipoprotein cholesterol (
TC /
HDL - C ) and the significant increased levels of non - high density lipoprotein
cholesterol ( non -
HDL - C ) compared to controls which can be used as markers of
dyslipidemia and can also be used to predict the risk of
cardiovascular disease in
type 2 Diabetes Mellitus . -
pubmed
18 - Fluorine fluorodeoxyglucose positron emission tomography in the pretreatment evaluation of
thymic epithelial neoplasms : a metabolic biopsy confirmed by
Ki-67 expression . -
pubmed
OBJECTIVES : To investigate the usefulness of
18 - fluorine fluorodeoxyglucose (
( 18 ) F - FDG ) positron emission tomography / computed tomography ( PET -
CT ) in the pretreatment evaluation of
thymic epithelial neoplasms (
TENs ) . -
pubmed
CONCLUSIONS :
FDG PET -
CT can provide a useful tool in the preoperative work - up of
TEN , reflecting its proliferation capacity , as described also by the
Ki-67 expression . -
pubmed
The major components of C - CAF are oxidative tea
polyphenols (
OTP ) and
caffeine . -
pubmed
In this study , we treated MUCs with
trichostatin A (
TSA ) to determine whether
histone deacetylase inhibitor is able to stimulate the expression of epithelial genes in MUCs , an essential step for guiding mesenchymal - like MUCs to become sensory epithelial cells . -
pubmed
After 72 h of
TSA treatment , MUCs acquired epithelial - like features , which were indicated by increased expression of epithelial markers such as
Cdh1 ,
Krt18 , and
Dsp . -
pubmed
Additionally ,
TSA decreased the expression of mesenchymal markers , including
Zeb1 ,
Zeb2 ,
Snai1 , and
Snai2 , and prosensory genes
Lfng ,
Six1 , and
Dlx5 . -
pubmed
Moreover , the expression of the hair
cell genes Atoh1 and
Myo6 was increased in
TSA - treated MUCs . -
pubmed
We also observed significantly decreased expression of
Hdac2 and
Hdac3 in
TSA - treated MUCs . -
pubmed
However , no remarkable change was detected in protein expression using immunofluorescence , indicating that
TSA - induced
HDAC inhibition may contribute to the initial stage of the mesenchymal - to - epithelial phenotypic change . -
pubmed
Nucleotide comparison revealed no significant similarity between
bsh1 and
bsh2 genes , whereas the deduced
amino acid sequences showed 26 % sequence similarity between both
BSH1 and
BSH2 . -
pubmed
Furthermore , examination of metabolic intermediates by gas chromatography - mass spectrometry revealed that the
naphthalene metabolic pathway in transgenic plants mainly involves the
dioxygenase pathway . -
pubmed
Taken together , our findings suggest that grafting the
naphthalene dioxygenase complex into plants is a possible strategy to breed
PAH - tolerant plants to efficiently degrade
PAHs in the environment . -
pubmed
Using
collagen ultrafiltration and single
amino acid (
hydroxyproline ) methods , these specimens consistently date to beyond or near the ~ 50,000 y B.P. -
pubmed
OBJECTIVE : To evaluate the efficacy and safety of the combination of
steroids , plasmapheresis and intravenous
immunoglobulins (
IVIG ) on maternal anti
Ro / SS - A antibody levels in cases of fetal cardiac involvement . -
pubmed
MATERIAL AND METHODS : A series of three cases of positive anti -
Ro / SS - A mothers with fetuses showing mild
cardiac involvement were treated with a triple therapy composed of
steroids , plasmapheresis and IVIG . -
pubmed
Caffeine and
caffeic acid suppressed the growth of
ER(+ ) ( P & lt;= 0.01 ) and
ER(- ) ( P & lt;= 0.03 ) cells . -
pubmed
Caffeine significantly reduced
ER and
cyclin D1 abundance in ER(+ ) cells . -
pubmed
Caffeine also reduced the
insulin - like growth factor - I receptor (
IGFIR ) and
pAkt levels in both
ER(+ ) and ER(- ) cells . -
pubmed
CONCLUSIONS : The clinical and experimental findings demonstrate various anticancer properties of
caffeine and
caffeic acid against both
ER(+ ) and
ER (- ) breast cancer that may sensitize
tumor cells to
tamoxifen and reduce
breast cancer growth . -
pubmed
RESULTS : For the
rocuronium - induced
neuromuscular block , the mean recovery time of the T4 /
T1 ratio to 0.9 decreased from 66.9 min in the
sugammadex 0.5 mg kg-1 group to 1.3 min in the
sugammadex 8.0 mg kg-1 group . -
pubmed
Implementation of diesel particulate filters for goods vehicles in the 2021 -
ALT -
IV - O scenario would help in the reduction of ~87 % of PM2.5 concentration , compared to the 2021 - BAU scenario ; translating into a gain of 1267 and 505 DALY per million people from exposure to PM2.5 and
NO2 concentrations , respectively . -
pubmed
After calculating these spectral indices based on the resampling
TM and Hyperion data , the regression equation between spectral indices and
chlorophyll content was established . -
pubmed
For
TM , the result indicates that VIUPD has the best correlation with
chlorophyll (
R2 = 0.819 7 ) followed by NDVI (
R2 = 0.791 8) , while MCARI / OSAVI and TVI also show a good correlation with
R2 higher than 0.5 . -
pubmed
It was demonstrated that VIUPD has the best accuracy and stability to estimate
chlorophyll of winter wheat whether using simulated
TM data or Hyperion data , which reaffirms that VIUPD is comparatively sensor independent . -
pubmed
Simple correlation analyses showed that serum
pentosidine levels were significantly and negatively correlated with muscle mass of legs ( r=-0.21 , p=0.017 ) and RSMI ( r=-0.18 , p=0.022 ) , and that
IGF - I was significantly and positively correlated with muscle mass of arms and legs ( r=0.23 , p=0.008 and r=0.30 , p=0.001 , respectively ) as well as RSMI ( r=0.20 , p=0.022 ) . -
pubmed
Moreover , after adjusting for age , duration of
diabetes , serum
creatinine , HbA1c , and
IGF - I ,
pentosidine was significantly and negatively associated with RSMI ( beta=-0.27 , p=0.018 ) and marginally with muscle mass of legs ( beta=-0.18 , p=0.071 ) . -
pubmed
The associations between
IGF - I and indices of muscle mass such as arms , legs and RSMI were still significant after additional adjustment for
pentosidine ( p=0.016 , 0.019 and 0.021 , respectively ) . -
pubmed
These findings indicate that increased serum
pentosidine and decreased
IGF - I are independent risk factors for
loss of muscle mass in postmenopausal women with
type 2 diabetes . -
pubmed
Randomized Controlled Trial of
Zoledronic Acid plus Chemotherapy versus Chemotherapy Alone as Neoadjuvant Treatment of
HER2 - Negative Primary
Breast Cancer ( JONIE Study ) . -
pubmed
We performed the present randomized Phase 2 trial to investigate the antitumor effect of
ZOL with chemotherapy (
CT ) . -
pubmed
Sonochemical
enzyme - catalyzed regioselective acylation of
flavonoid glycosides . -
pubmed
The data suggested that monoclonal
sclerostin - neutralizing antibody represents a promising therapeutic approach for severe
osteoporosis induced by
estrogen deficiency with concurrent mechanical unloading . -
pubmed
However , unlike other
HSPs such as
HSP90 and
HSP70 , small molecule approaches for neutralization of
HSP27 are not well established because of the absence of an
ATP binding domain . -
pubmed
Previously , we found that a small molecule ,
zerumbone (
ZER ) , induced altered dimerization of
HSP27 by cross linking the
cysteine residues required to build a large oligomer , led to sensitization in combination with radiation . -
pubmed
Dysregulation of
matriptase expression , an epithelial - specific member of the type II transmembrane
serine protease family , has been demonstrated in many different
cancer types . -
pubmed
Mass yield of
mevalonate was increased by 41 % , and production was maintained for more than 45h after activation of the
pyrF - based growth switch . -
pubmed
Lipidomic analyses of the two mutants revealed significant and specific declines in
phosphatidylinositol and
phosphatidylglycerol levels upon repression of
TgCDS1 and after deletion of
TgCDS2 , respectively . -
pubmed
Here we show that perturbations in equilibrium picosecond - nanosecond motions impact
zinc (
Zn)-induced allosteric inhibition of DNA binding by the
Zn efflux repressor
CzrA (
chromosomal zinc - regulated repressor ) . -
pubmed
Allosterically impaired
CzrA mutants are characterized by distinct nonnative fast internal dynamics " fingerprints " upon
Zn binding , and DNA binding is weakly regulated . -
pubmed
These findings identify a unique amplification process of
TNF synthesis and secretion via the interaction of
NK-1 and
ST2 receptors inhibitable by
methoxyluteolin . -
pubmed
Three sequence - related amplified polymorphism (
SRAP ) markers were employed to fingerprint genomic DNA , and the amounts of
mitraphylline and
isomitraphylline in leaf samples were established by high - performance liquid chromatography . -
pubmed
Mn - induced mitochondria - related apoptotic characteristics , such as
caspase-3 and - 9 activation ,
cytochrome c release ,
Bax increase , and
Bcl-2 decrease , were significantly suppressed by
curcumin . -
pubmed
In addition ,
curcumin significantly increased intracellular
glutathione (
GSH ) and moderately potentiated
superoxide dismutase (
SOD ) , both which were diminished by
Mn treatment . -
pubmed
Furthermore ,
curcumin was able to induce
heme oxygenase ( HO )- 1 expression . -
pubmed
Curcumin - mediated inhibition of ROS , down - regulation of
caspases , restoration of
MMP , and recovery of cell viability were partially reversed by
HO-1 inhibitor (
SnPP ) . -
pubmed
These results suggest the first evidence that
curcumin can prevent
Mn - induced microglial cell
death through the induction of
HO-1 and regulation of oxidative stress ,
mitochondrial dysfunction , and apoptotic events . -
pubmed
Stimulatory Action of
Telmisartan , an Antagonist of
Angiotensin II Receptor , on Voltage - Gated
Na + Current : Experimental and Theoretical Studies . -
pubmed
Telmisartan (
Tel ) is recognized as a non - peptide blocker of
AT1R. -
pubmed
Mechanistically ,
Snhg8 sustained SCs proliferation by promoting the transcription of ribosomal proteins , while
Gm26917 acted as a competing endogenous RNA for microRNA-29b , which accelerated apoptosis of SCs . -
pubmed
Forward contamination is prevented with sealed tubes , and the use of thermolabile
uracil DNA glycosylase ( UDG ) in the presence of
dUTP in the amplification mixture . -
pubmed
A long - lasting oral preformulation of the
angiotensin II AT1 receptor antagonist
losartan . -
pubmed
Losartan (
Los ) , a non - peptidic orally active agent , reduces arterial pressure through specific and selective blockade of
angiotensin II receptor AT1 . -
pubmed
Here , we show that native
transition metals (
Fe0 ,
Ni0 and
Co0 ) selectively reduce
CO2 to
acetate and
pyruvate - the intermediates and end - products of the
AcCoA pathway - in near millimolar concentrations in water over hours to days using 1 - 40 bar
CO2 and at temperatures from 30 to 100 C. Geochemical
CO2 fixation from native metals could have supplied critical
C2 and C3 metabolites before the emergence of
enzymes . -
pubmed
The present study for the first time gives an insight into regulation of
estrogen - related receptors by histone methylation specifically through methyltransferase
EZH2 in
breast cancer . -
pubmed
Immunohistochemistry in primary breast
tumors of different grades showed a correlated expression of
estrogen - related receptors and
EZH2 . -
pubmed
Ectopic expression of
EZH2 in low
EZH2 - expressing
normal breast epithelial cells abrogated their expression and at the same time , its silencing enhanced the expression of
estrogen - related receptors in cancerous cells . -
pubmed
IE measurements with liquid chromatography electrospray ionization mass spectrometry ( LC / ESI /
MS ) were carried out , and IE scales were constructed with a calibration curve for compounds with and without derivatization reagent
diethyl ethoxymethylenemalonate . -
pubmed
Abrogation of the
Circadian Nuclear Receptor REV - ERBalpha Exacerbates
6 - Hydroxydopamine - Induced Dopaminergic
Neurodegeneration . -
pubmed
REV -
ERBalpha deficiency significantly exacerbated
6 - OHDA - induced motor deficits as well as DAergic neuronal loss in the vertebral midbrain including the
SN and the ventral tegmental area . -
pubmed
In conclusion , the present study demonstrates for the first time that genetic abrogation of
REV - ERBalpha can increase vulnerability of DAergic neurons to
neurotoxic insults , such as
6 - OHDA , thereby implying that its normal function may be beneficial for maintaining DAergic neuron populations during
PD progression . -
pubmed
Decreased reduction rate of the
luciferase flavin substrate was shown to be a possible reason for bioluminescence quenching . -
pubmed
Furthermore ,
ATG7 knockdown led to an increased survival fraction under
oxygen and
glutamine starvation , indicating that ionizing radiation indeed induces autophagy which , however , leads to cell
death finally . -
pubmed
Van der Waals heterostructures composed of two - dimensional ( 2D )
transition metal dichalcogenides (
TMD ) materials have stimulated tremendous research interest in various device applications , especially in energy - efficient future - generation electronics . -
pubmed
Long - term improvement in renal , bone , lipid parameters , and
CD4 /
CD8 ratio in
HIV - infected patients switching to a dual therapy with
lamivudine plus boosted
darunavir . -
pubmed
Thiol -
norbornene photoclick hydrogels were formed with CRGDS and crosslinkers of
PEG dithiol ( nondegradable ) ,
CVPLS - LYSGC (
P1 ) or
CRGRIGF - LRTDC (
P2 ; dash indicates cleavage site ) at two crosslink densities . -
pubmed
Overall , the low crosslinked
P1 hydrogels exhibited an accelerated early and late osteogenesis with the highest
ALP activity ( Day 7 ) , greatest
calcium content ( Day 14 ) , and greatest
collagen content ( Day 28 ) , concomitant with increased compressive modulus over time . -
pubmed
The infant grew rapidly with rising
PRL ,
GH , and IGF-1 concentrations and an enlarging suprasellar pituitary mass , despite treatment with
bromocriptine . -
pubmed
Here we predict two monolayered
aluminum triphosphides (
AlP3 ) with
C2 / m and
P3m1 space groups with a tunable bandgap under
strain as the new members of the 2D
XP3 family by using the first principles calculations . -
pubmed
The high mobility of 1.01 x 105 and 1.62 x 104 cm2 V-1 s-1 are observed for the electron of
P3m1 and the hole of
C2 / m. The optical absorptions of the
AlP3 monolayers , in particular , the one with
C2 / m , are obviously strong in the visible light range . -
pubmed
The three - year concentration of PM2.5 , PM10 and
NO2 was 72.8 + - 2.3 mug / m3 , 131.5 + - 5.7 mug / m3and
39.1 + - 3.1 mug / m3 , respectively . -
pubmed
Complete ( 100 % ) inhibition of biofilm formation was observed with plain
CuO NP ( 0.5 M
hexamine , uncapped ) at 1,000 microg ml-1 . -
pubmed
In this study , we evaluated the effects of
PPB effect
M1 polarization and
inflammation and its ability to restore the effects of
leptin , such as a decrease in appetite and
body weight . -
pubmed
Losatuxizumab vedotin ( formerly
ABBV-221 ) is a second - generation antibody - drug conjugate targeting
epidermal growth factor receptor (
EGFR ) . -
pubmed
The
NLRP3 (
nucleotide - binding domain ,
leucine - rich - repeat - containing family , pyrin domain - containing 3 ) inflammasome senses pathogen - associated molecular patterns ( PAMPs ) and danger - associated molecular patterns ( DAMPs ) , and activates
caspase-1 , which provokes release of proinflammatory
cytokines such as
interleukin-1beta (
IL-1beta ) and
IL-18 as well as pyroptosis to engage in innate immune defense . -
pubmed
ER - mitochondria contact sites provide a location for
NLRP3 activation , its association with ligands released from or residing in mitochondria , and rapid
Ca2 + mobilization from
ER stores to mitochondria . -
pubmed
Lipid perturbation and
cholesterol trafficking to the
ER activate the
NLRP3 inflammasome . -
pubmed
Serum
NO and
H2O2 concentrations increased significantly and were related to
muscle damage (
CK activity > 900 IU / mL ) in IHE and
Arg / IHE compared to placebo . -
pubmed
The changes in
NO and
H2O2 elevated the levels of circulating growth factors such as
HGF ,
IHG-1 ,
PDGFBB ,
BDNF ,
VEGF and
EPO . -
pubmed
Pembigatinib has become the first approved targeted therapy for
BTC with
FGFR2 fusion or other rearrangements . -
pubmed
Recent immunotherapy has opened new therapy avenues in
BTC with
pembrolizumab approved for either
microsatellite instability high (
MSI - H ) or
DNA mismatch repair deficient (
dMMR ) advanced
solid tumors , including
BTC . -
pubmed
Excellent therapeutic success of the anti -
interleukin 4 receptor - alpha antibody
dupilumab in clinical trials and a real - world clinical context indicates the crucial roles of
interleukin ( IL )- 4 and
IL-13 in the pathogenesis of
AD . -
pubmed
Along with the clinical improvement in skin scores and
pruritus ,
dupilumab significantly and progressively reduces and normalizes the upregulated expression of T helper type 2 signatures such as
Chemokine ( C - C motif ) ligand ( CCL ) 17 ,
CCL18 ,
CCL22 , and
CCL26 in the lesional skin of
AD . -
pubmed
Herein , we report a nano -
MOF conjugated to
maltotriose as a new DDS . -
pubmed
This novel
MOF is an effective PDT agent and shows little dark toxicity , MA -
PCN-224 - 0.1Mn/0.9Zn uptakes selectively into
cancer cells . -
pubmed
Aberrant
ALDH5A1 promoter regulation in functional terms can contribute to impaired
gamma - aminobutyric acid homeostasis and thereby network excitability and
seizure propensity . -
pubmed
The inhibition effect of
N , N '- phosphonomethylglycine (
PMG ) and
vinyl phosphonic acid (
VPA ) on the 3 %
NaCl acidic solution corrosion of
carbon steel
iron was studied at different immersion times by potentiodynamic polarization , electrochemical impedance spectroscopy , attenuated total reflectance Fourier transform infrared (
ATR - FTIR ) spectroscopy , and computational methods . -
pubmed
Plasma biochemical analytes included
glucose (
Glu ) , total
calcium ,
phosphorus ,
sodium ,
potassium ,
chloride , blood
urea nitrogen (
BUN ) ,
uric acid ,
cholesterol ,
triglycerides ,
creatine kinase ,
gamma - glutamyl transferase ,
alkaline phosphatase (
ALP ) ,
lactate dehydrogenase ,
aspartate aminotransferase ,
amylase , and
bile acids . -
pubmed
Moreover , IMO decreased the concentrations of serum
D - lactate (
D - LA ) and lipopolysaccharides ( LPS ) at farrowing and day 18 of lactation (
L18 ) ( P < 0.05 ) . -
pubmed
At 48 h before
CIDR withdrawal , does were treated with 80 mg
follicle - stimulating hormone (
FSH ) administered at 12 h intervals . -
pubmed
Carbon black filled only the pial arteries and the
rest of the brain was totally unperfused . -
pubmed
Effects of dietary
magnesium supplementation on diurnal variations of blood pressure and plasma
Na + , K (+)- ATPase activity in essential
hypertension . -
pubmed
Supplementation of
MgO elicited a significant fall in averaged mean blood pressure calculated with a 24 - h ambulatory blood pressure monitoring system (
MBP ) in EH from a baseline value of 104.3 + /- 12.2 to 99.5 + /- 11.6 mmHg ( p & lt ; 0.05 ) , while controls remained unaltered from a baseline value of 85.1 + /- 11.5 to 84.5 + /- 13.3 mmHg . -
pubmed
After 2 weeks of
magnesium supplementation , the plasma level of
Na + , K (+)- ATPase inhibitory activity ( PATPI ) , defined as equivalency to
ouabain , was reduced significantly from 0.75 + /- 0.54 to 0.40 + /- 0.30 mumol
ouabain / ml ( p & lt ; 0.05 ) in the responder group , while it remained unaltered in controls and the nonresponder group . -
pubmed
A significant negative correlation existed between the pretreatment PRA and changes in
MBP after
magnesium supplementation in EH ( r = -0.65 , p & lt ; 0.01 ) , and there was a significant positive correlation between changes in PATPI and changes in
MBP as a whole ( r = 0.41 , p & lt ; 0.05 ) . -
pubmed
It appears that
magnesium suppresses circulating
Na + , K (+)- ATPase inhibitory activity to attenuate vascular tone , and thereby reduces blood pressure in
EH . -
pubmed
The
enzyme displayed the maximum activity towards
proline p - nitroanilide at pH 7.7 - 7.9 and readily split
glycine ,
alanine and
proline from
di - , tri - and tetrapeptides but did not practically hydrolyze oligopeptides of a greater chain length . -
pubmed
The
enzyme was not inhibited by complexons (
EDTA ,
8 - oxiquinoline and 1.10 -
phenanthroline ) . -
pubmed
The
peptidase was not activated by divalent metal ions and was inhibited by
Zn2 + ;
Cd2 + ,
Hg2 + and
Cu2 + . -
pubmed
In the presence of
sodium dodecyl sulfate the molecular mass of the
enzyme is 43 kDa , which is suggestive of the presence of a quaternary structure . -
pubmed
One peculiarity of the
enzyme is its activation by
alkaline metal halogenides and
sodium nitrate which reaches a maximum at the 0.05 - 0.1 M concentration of the salts . -
pubmed
Under conditions of exposure where both chemicals elicited an
IgG anti - hapten antibody response , only
phthalic anhydride caused an increase in the serum concentration of
IgE. -
pubmed
Furthermore , like
TMA ,
phthalic anhydride preferentially induced
IgG2b rather than
IgG2a antibody . -
pubmed
In contrast ,
oxazolone , like
DNCB , induced a markedly stronger
IgG2a than
IgG2b antibody response . -
pubmed
The
HOX4A protein predicted from the nucleotide sequence of the
HOX4A gene is comprised of 416
amino acid residues . -
pubmed
A dose finding study of a super long - acting
luteinizing hormone - releasing hormone analog (
leuprolide acetate depot ,
TAP-144 - SR ) in the treatment of
central precocious puberty . -
pubmed
The lack of
PLA2 in salt extracts from newborn cartilage observed by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis analysis was confirmed by the very low levels of
PLA2 activity detectable in these preparations . -
pubmed
ER mRNA containing cells were found in the periventricular , suprachiasmatic and medical preoptic cell groups , in a manner which agrees with
steroid hormone autoradiography . -
pubmed
We conclude that the insensitivity to the male to
progesterone , as regards the hormonal control of ovulation , can not be due to a total failure of
PR gene expression in a specific subset of POA neurons . -
pubmed
These patients were mostly men , had a family history of premature
CAD , and 60 % ( 414 of 690 ) had
high - density lipoprotein (
HDL )
cholesterol less than 0.90 mmol / liter ( 35 mg / dl ) . -
pubmed
In a separate group of patients defined from the same admission population but having angiographically established
CAD , 32 % ( 424 of 1,197 ) had a total
cholesterol less than 5.17 mmol / liter ( 200 mg / dl ) , 59 % of whom ( 252 of 424 ) had
HDL less than 0.90 mmol / liter ( 35 mg / dl ) . -
pubmed
An analysis of persons admitted electively for angiography ( to exclude any effects of hospitalization per se on serum lipids ) revealed a similar proportion of persons with total
cholesterol less than 5.17 mmol / liter ( 200 mg / dl ) ( 35 % ) ,
CAD ( 82 % ) , and
HDL less than 0.90 mmol / liter ( 35 mg / dl). -
pubmed
With straight chain
aliphatic aldehydes (
C1 - C6 ) the Km values of the
E3 isozyme are identical with those of the
E1 isozyme . -
pubmed
All
isozymes dehydrogenate naturally occurring
aldehydes ,
5 - imidazoleacetaldehyde (
histamine metabolite ) and
acrolein ( product of beta - elimination of oxidized
polyamines ) with similar catalytic efficiency . -
pubmed
Differences between the
isozymes are in the Km values for
aminoaldehydes . -
pubmed
Although all
isozymes can dehydrogenate
gamma - aminobutyraldehyde , the Km value of the
E3 isozyme is much lower : the same appears to apply to
aldehyde metabolites of
cadaverine ,
agmatine ,
spermidine , and
spermine for which Km values range between 2 - 18 microM and kcat values between 0.8 - 1.9 mumol / min / mg . -
pubmed
Thus , the
E3 isozyme has properties which make it suitable for the metabolism of
aminoaldehydes . -
pubmed
The physiological role of
E1 and
E2 isozymes could be in dehydrogenation of
aldehyde metabolites of
monoamines such as
3,4 - dihydroxyphenylacetaldehyde or
5 - hydroxyindoleacetaldehyde ; the catalytic efficiency with these substrates is better with
E1 and
E2 isozymes than with
E3 isozyme . -
pubmed
Although the
enzyme is generally considered to function in detoxication , our data suggest an additional function in metabolism of biogenic
amines . -
pubmed
Mossy fibres form synapses with identified pyramidal basket cells in the
CA3 region of the guinea - pig hippocampus : a combined Golgi -
electron microscope study . -
pubmed
By using the combined Golgi -
electron microscope technique mossy fibre terminals were found , which established asymmetric synaptic contacts with both spines of pyramidal cells and smooth dendrites of identified ( Golgi - stained ) pyramidal basket cells in the
CA3 region of the guinea - pig hippocampus . -
pubmed
A 57 kd component of
oligosaccharyl transferase , termed
glycosylation site binding protein , specifically recognizes a photoaffinity probe containing the N - glycosylation site sequence
Asn - Lys - Thr . -
pubmed
Corticotropin - releasing factor (
CRF ) and
dynorphin - related peptides are co - localized within a subset of hypothalamic neurons suggesting the possibility of their co - release . -
pubmed
Upon simultaneous administration , 0.1 nmol of
dynorphin A1 - 17 , but not 0.1 nmol of
dynorphin A1 - 8 or
dynorphin A1 - 13 , markedly attenuated
CRF - induced elevations of AP , HR , and plasma
catecholamine levels . -
pubmed
The activity changes of
lactate dehydrogenase reflected changes in tissue
lactate levels and
succinate dehydrogenase activity had a tissue specificity . -
pubmed
GTP - binding proteins appear to couple receptors to
phospholipase - C - mediated PPI breakdown . -
pubmed
Fluoride ( 1 - 30 mM ) also inhibited
PTH release in dose - dependent fashion . -
pubmed
Fluoride ( 30 mM ) produced 72.8 + /- 4.2 % suppression of maximal low
Ca2 + -stimulated
PTH release comparable to the 83.7 + /- 3.7 % inhibition by 2.0 mM extracellular
Ca2 + . -
pubmed
Since changes in both [
Ca2+]i and
cAMP regulate
PTH release , we measured the effect of
fluoride on intracellular
cAMP . -
pubmed
While both may contribute to the
fluoride - induced suppression of
PTH release , our findings suggest that the stimulation of PPI turnover leads to inhibition of
PTH secretion . -
pubmed
Phy is metabolized to
eseroline and three other metabolites ,
M1 , M2 , and M3 . -
pubmed
The maximum inhibition of
BuChE ( 52 % ) correlates with the highest
Phy concentration ( 84.6 ng / ml ) in plasma at 2 min and 70 % of the enzymic activity recovered by 45 min . -
pubmed
Previous acute studies of intratracheal cannulation ( ITC ) versus intralaryngeal cannulation (
ILC ) showed that tracheal cell proliferation increased significantly in
ITC - induced mucosal
wounds . -
pubmed
Only mild increases in intrapulmonary cell proliferation were produced by
Fe2O3 - saline given by
ILC or ITC ( Keenan et al . -
pubmed
The present chronic studies included the following variables : a single instillation by
ILC of
N - methyl - N - nitrosourea (
MNU ) at 5 weeks of age ; 15 weekly treatments ( beginning at 7 weeks of age ) by
ILC or
ITC alone , or with instillations of saline , or
Fe2O3 - saline , or BP -
Fe2O3 - saline ; and appropriate controls . -
pubmed
Our conclusions are as follows : Plasma
beta - endorphin shows a circadian rhythm similar to that seen with
corticotropin (
ACTH ) and is suppressable by
dexamethasone . -
pubmed
When abnormality of either the
beta - endorphin or
cortisol is considered it is clear that both levels of the hypothalamic - pituitary - adrenal axis can be dysregulated in endogenous
depression . -
pubmed
An improved method is described in which toxic
APAP metabolites are generated by a purified and reconstituted
cytochrome P-450 system , minimizing the amount of exogenous detoxification enzymes in the assay . -
pubmed
Toxicity required
cytochrome P-450 and
NADPH , and was inhibited by
SKF 525A. -
pubmed
The data are consistent with the generation of
cytochrome P-450 - dependent reactive metabolites which subsequently can be detoxified by
glutathione . -
pubmed
Exostoses have already been studied and explained by the action of
BAPN (
lysyl oxidase ) . -
pubmed
Release was determined by assay of biochemical markers (
serotonin for dense granules and
beta - thromboglobulin for alpha - granules ) . -
pubmed
A tenfold higher concentration of
prostacyclin was required to inhibit completely
serotonin release as compared to the concentration required for
beta - thromboglobulin release . -
pubmed
The decrease in toxin - binding ability of synaptosomes was paralleled by a release of
sialic acid from the synaptosomes by the
neuraminidase treatment . -
pubmed
The toxin - binding ability of synaptosomes treated with
neuraminidase was lower than untreated ones at a high concentration of
sodium chloride . -
pubmed
In contrast ,
17 alpha - OAc and especially
21 - OAc substitution on
hydrocortisone and
betamethasone causes a decrease in the
steroid affinity for the
receptor and an increase in
steroid lipophilicity . -
pubmed
The elongation of the
ester chain from
acetate to
valerate in both position C-17 and C-21 leads to the increase in both the binding affinity for the
receptor and the lipophilicity of
steroids . -
pubmed
Only in the series of 17 alpha- and
21 - esters is there a correlation between the binding affinity of
steroids for the
glucocorticoid receptor and their lipophilicity . -
pubmed
An investigation of
Ca2 + -binding centers of
calmodulin was carried out by EXAFS - spectroscopy . -
pubmed
The experimental results for
protein preparations of
calmodulin in which
Ca2 + was isomorphically replaced by
Tb3 + were obtained by a spectrometer working at the Institute of Nuclear Physics . -
pubmed
Coincidence main maxima on phi ( r ) curves and identity of Fourier transformation for
calmodulin and
parvalbumin in the 2 - 6 A interval allow to infer the identity of
Ca2 + -binding centers of
calmodulin and
parvalbumin . -
pubmed
Utilization of
pppGpp and
dGTP by
initiation factor 2 and
elongation factor Tu . -
pubmed
Histidyl groups seem to be located in the active site of the
dicarboxylic transporter in that the simultaneous presence of
Na and
succinate protects the transporter against the
histidyl specific reagent
diethylpyrocarbonate . -
pubmed
Lithium , which specifically competes for
sodium sites in the
dicarboxylic acid transporter , substantially blocked the protective effect of
Na and
succinate . -
pubmed
We conclude that a
histidyl residue is at , or is close to , the active site of the
dicarboxylate transporter in renal brush border membranes . -
pubmed
The activities of
sialyl- and galactosyltransferases towards endogenous acceptors in the synaptic junction fraction were low but increased greatly after the addition of deglycosylated
fetuin or
mucin as exogenous acceptors . -
pubmed
Hydroperoxides are determined from the coupled activities of
glutathione peroxidase and
glutathione reductase as measured by the loss of
NADPH absorbance . -
pubmed
To minimize the effects of tissue catalyzed
peroxide degradation , incubations were performed in the presence of
1,3 - bis ( 2 - chloroethyl )- 1 - nitrosourea (
BCNU ) ; which inhibited the activity of retinal tissue
glutathione reductase by 85 % . -
pubmed
Rat brain transketolase and
pyruvate dehydrogenase were inhibited 25 percent by 10 - 9 M
moniliformin . -
pubmed
Studies carried out to determine if
moniliformin causes
enzyme inhibition by reaction with
thiamine were negative . -
pubmed
After treatment with
macrolide antibiotics , a significant reduction in the number of lymphocytes and activated
CD8 + cells and an elevation in the
CD4 /
CD8 ratio in BALF of
DPB patients was observed . -
pubmed
Similarly , the content of
alpha - hydroxyaldehydes produced from
plasmalogens via their epoxids during lipidperoxidation (
LPO ) was studied . -
pubmed
VLDL and
LDL plasmalogens were oxidized to a greater extend ( 99 % decrease ) compared to
HDL plasmalogens ( 35 % ) . -
pubmed
Specificity of the binding of
E3 to
E2 was certified by a competition experiment measuring binding of
biotin - labeled
E3 in the presence of unlabeled
E3 . -
pubmed
Influence of monovalent cations on the ultraviolet - visible spectrum of
tryptophan tryptophylquinone - containing
methylamine dehydrogenase from bacterium
W3A1 . -
pubmed
Except for
NH4 + , all spectral changes produced by binding of the monovalent cations can be explained by assuming two different binding sites in
MADH ( type I and type
II sites ) . -
pubmed
Na + and
K + displayed monophasic binding to the type
II site ,
( CH3 ) 3NH+ and
( CH3 ) 4N+ displayed monophasic binding to the type I site , and
Li + ,
Rb + , and
Cs + displayed either monophasic or biphasic binding to one or both sites depending on pH. -
pubmed
CA III activity was significantly increased in SS - treated
EDL and TA muscles compared to control ( CTR ) , while
SNE injections partially prevented this increase . -
pubmed
The
CA III mRNA increase observed in response to denervation was reduced by 40 % in
SNE - treated
EDL and TA muscles . -
pubmed
Heart rate (
HR ) , contractility ( dP / dt ) , mean arterial blood pressure ( MABP ) , left ventricular pressure ( LVP ) , coronary
sinus pressure ( CSP ) , left - ventricular end -
diastolic pressure ( LVEDP ) , coronary blood flow (
CBF ) , coronary vascular resistance ( CVR ) , myocardial
oxygen consumption ( MVO2 ) and coronary
sinus lactate were determined after 20 min in each position . -
pubmed
A common
amino acid polymorphism in
insulin receptor substrate-1 causes impaired
insulin signaling . -
pubmed
As compared to wild - type
IRS-1 ,
insulin stimulation of cells transfected with mutant
IRS-1 exhibited a 32 % decrease in incorporation of
[ 3H ] thymidine into DNA ( P = 0.002 ) , a 36 % decrease in
IRS-1 associated
phosphatidylinositol ( PI ) 3 - kinase activity ( P = 0.004 ) and a 25 % decrease in binding of the
p85 regulatory subunit of
PI 3 - kinase to
IRS-1 ( P = 0.002 ) . -
pubmed
The changes occurred with no change in
insulin receptor or
IRS-1 tyrosine phosphorylation . -
pubmed
Two crystal structures of the
leupeptin -
trypsin complex . -
pubmed
Three - dimensional structures of
trypsin with the reversible inhibitor
leupeptin have been determined in two different crystal forms . -
pubmed
The
leupeptin backbone forms four
hydrogen bonds with
trypsin and a fifth
hydrogen bond interaction is mediated by a water molecule . -
pubmed
The reaction of
trypsin with
leupeptin proceeds through the formation of stable tetrahedral complex in which the
hemiacetal oxygen atom is pointing out of the
oxyanion hole and forming a
hydrogen bond with
His-57 . -
pubmed
lividans (
ard2 ) were shown to phosphorylate A201A in an
ATP - dependent reaction . -
pubmed
Phosphorylation by
Ard2 takes place on the
hydroxyl group at
C2 of the unsaturated
hexofuranose moiety of A201A , as shown by
1H - NMR analysis of purified
P - A201A. -
pubmed
Since unlike their normal counterparts , triggering of their
TCR / CD3 in vitro elicits only a weak
mitogenic response , we set out to determine which of the
signal transduction molecules initiated by
anti - CD3E antibodies are affected in neoplastic cells . -
pubmed
The results obtained from analysis of
tumor cells from four patients show a general reduction in basal and induced
tyrosine phosphorylation of a wide range of
signaling proteins . -
pubmed
SurP , like other
sucrose EIIs , lacked the hydrophilic domain containing the first ( IIA ) phosphorylation site . -
pubmed
Cells deleted for
rsv1 + are unable to survive
glucose starvation . -
pubmed
Transcription of
rsv1 + is negatively regulated by the
cAMP pathway and induced by
glucose starvation . -
pubmed
The
nitric oxide synthase inhibitor
N ( G )- nitro - L - arginine , ( 100 microM ) reduced
[ 3H ] L - citrulline production in preparations from all three species . -
pubmed
Omission of
calcium or
NADPH significantly reduced
nitric oxide synthase - like activity in preparations from all three species but
arginase - like activity was not significantly affected . -
pubmed
These results provide direct evidence that the two conserved cationic residues
Arg33 and
Lys27 , located on loop
II of the toxin structure , are binding in close proximity to the alpha - subunit region between residues 188 - 200 . -
pubmed
This is supported by a number of experimental findings : ( i )
NiR - deficient mutants have lost
Nii1 gene expression ; ( ii )
Nii1 mRNA accumulation is co - regulated with the expression of other structural genes of the
nitrate assimilation pathway ; ( iii )
nitrite (
nitrate ) utilization ability is recovered in the
NiR mutants by functional complementation with a wild - type
Nii1 gene ; ( iv ) the elucidated
NII1 amino acid sequence is highly similar to that of the cyanobacterial and higher - plant enzyme , and contains the predicted domains for plastidic
ferredoxin -
NiRs . -
pubmed
Lde1 ( light - dependent expression ) , was not regulated by
nitrogen , but like
nitrate - assimilation clustered genes , its expression was down - regulated in the dark . -
pubmed
Inactivation of
cytochrome P450 2E1 by
tert - butylisothiocyanate . -
pubmed
Several naturally occurring and synthethic
isothiocyanates were evaluated for their ability to inactivate the major
ethanol - inducible hepatic
cytochrome P450 2E1 . -
pubmed
Of the compounds tested ,
tert - butylisothiocyanate (
tBITC ) was found to be the most selective inactivator of the
2E1 p - nitrophenol hydroxylation activity . -
pubmed
The kinetics of inactivation of
P450 2E1 by
tBITC were characterized . -
pubmed
When
cytochrome b5 was co - reconstituted with
P450 2E1 , the apparent KI for
P450 2E1 inactivation by
tBITC was similar to that seen in microsomes ( 14 microM ) . -
pubmed
P450 2E1 T303A was also inactivated by
tBITC with kinetic constants similar to that of the wild type
enzyme . -
pubmed
Co - incubations with an alternate substrate protected
P450 2E1 from inactivation by
tBITC . -
pubmed
The extent of
P450 2E1 inactivation by
tBITC resulted in a comparable loss of the ability of the
enzyme to form a reduced
CO complex . -
pubmed
Although
angiotensin receptor antagonists (
AII - A ) share the common property with
ACE - I with regard to blockade of
angiotensin activity via
angiotensin type 1 receptors (
AT1 ) ,
AII - A is also reported to stimulate
AT2 that plausibly activates
nitric oxide production within renal medulla and augments synthesis of vasodilatory
P450 - metabolites in renal afferent arterioles . -
pubmed
In contrast ,
AII - A is reported to have no effect on
bradykinin activity . -
pubmed
beta - adrenergic blockade in developing
heart failure : effects on myocardial inflammatory
cytokines ,
nitric oxide , and remodeling . -
pubmed
BACKGROUND : Whether beta - adrenergic blockade modulates myocardial expression of inflammatory
cytokines and
nitric oxide (
NO ) in
heart failure is unclear . -
pubmed
Papillary muscle studies revealed
isoproterenol hyporesponsiveness to be unaltered by
NO synthase (
NOS ) inhibition . -
pubmed
Metoprolol administration starting 48 hours after
infarction ( 1 ) attenuated ( P<0.02 )
LV dilatation and
systolic dysfunction , ( 2 ) preserved
isoproterenol responsiveness ( P<0.025 ) via
NO - independent mechanisms , and ( 3 ) reduced myocardial gene expression and protein production of
TNF - alpha and
IL-1beta ( P<0 . -
pubmed
CONCLUSIONS : During
heart failure development , adrenergic activation contributes to increased myocardial expression of
TNF - alpha and
IL-1beta but not
IL-6 , and one mechanism underlying the beneficial effects of beta - adrenergic blockade may involve attenuation of
TNF - alpha and
IL-1beta expression independent of
iNOS and
NO . -
pubmed
The interactions between
alcohol and
cytochrome P-450 enzymes have been well investigated . -
pubmed
However , the data regarding the effect of
alcohol on the regulation of
UDP - glucuronosyltranferase (
UGT ) activity are less clear . -
pubmed
In cultured hepatocytes , treatment with either
ethanol or
isopentanol significantly increased the expression of
UGT1A1 ,
UGT1A5 ,
UGT2B1 , and
UGT2B3 mRNAs , but to different degrees . -
pubmed
The induction of
UGT1A1 and
UGT2B1 mRNAs by
ethanol or
isopentanol was time - dependent and maximal changes occurred at 48 h. The expression of
UGT1A6 mRNA was not significantly modified by either
ethanol or
isopentanol . -
pubmed
In conclusion ,
ethanol and
isopentanol have direct roles in the regulation of
UGT . -
pubmed
Lower levels of plasma
11 - ketotestosterone and higher levels of plasma
vitellogenin were shown in the E(2 ) group . -
pubmed
The method gives good quantitative agreement with standard Soxhlet extraction , and with certified reference materials for
PAH concentrations on soil , sediment (
SRM 1944 ) , and air particulate matter (
SRM 1649a ) . -
pubmed
Recently , PBSC collection after high - dose
cytarabine , with or without
G - CSF , has been associated with DFS ranging from 47 to 57 % . -
pubmed
In this study we investigated whether oral
prednisolone modulates the balance of peripheral blood
CXCR3 + and
CCR4 + T cells . -
pubmed
In the
steroid - treated
asthma group , there was a decrease in
CCR4 + T cells ( from 29.3 % to 20.3 % , p & lt ; 0.0001 ) , and an increase in
CXCR3+/
CCR4 + ratio ( from 1.86 to 2.89 , p = 0.0047 ) , whereas there was no change in
CXCR3 + T cells . -
pubmed
However , the percentages of
CCR4 + cells did not change after
steroid therapy in normal control subjects . -
pubmed
NADH - dependent
oxidase activity was shown to be equally induced ( 2- to 3 - fold ) in endothelial cells exposed to pulsatile unidirectional or oscillatory flow patterns . -
pubmed
P22phox mRNA level increased in cells exposed to both unidirectional and oscillatory shear stress , suggesting that
p22phox gene expression upregulation contributes to flow - induced increase in
superoxide anion production in endothelial cells . -
pubmed
We reinvestigated the structures of
mannobiose ( M(2 ) ) , M(3)s , and
mannotetraose ( M(4 ) ) that were completely isolated by matrix - assisted laser desorption ionization time - of - flight mass spectrometry ( MALDI -
TOF -
MS ) and NMR spectroscopy . -
pubmed
cruzi as evidenced by depolarization of the DeltaPsi by
H (+)- ATPase inhibitors
dicyclohexylcarbo - diimide ,
N - ethylmaleimide ,
diethylstilbestrol , and
bafilomycin A(1 ) . -
pubmed
However ,
ouabain induced an additional significant depolarization in these stages when their DeltaPsi was already partially depolarized by the
H (+)- ATPase inhibitor
dicyclohexylcarbo - diimide , supporting the presence of an
ouabain - sensitive
sodium pump whose activity is masked by the
H (+)- ATPase . -
pubmed
The presence of
K (+ ) channels in amastigotes and a
sodium pump in trypomastigotes , in addition to the
H (+)- ATPase , could provide important new targets for trypanocidal drug development . -
pubmed
We applied two - dimensional gel electrophoresis ( 2 - DE ) to the total exoproteins secreted from pathogenic
MRSA strains and identified major protein
spots by N - terminal
amino acid sequence analysis . -
pubmed
Here we find that there is a
cholesterol - dependent association between
CD45 and the raft - associated protein linker for activation of T cells , suggesting that
CD45 and linker for activation of T cells may colocalize in lipid rafts . -
pubmed
Consistent with this observation , we find that approximately 5 % of total
CD45 can be detected in
Triton X-100 - insoluble buoyant fractions of
sucrose gradients , demonstrating that
CD45 is not excluded from lipid rafts . -
pubmed
A
His - X -
His pseudotripeptide
zinc complex ( X is a
N - alkyl glycine derivative ) similar to the catalytic center of the
carbonic anhydrase was computer designed and experimentally synthesized . -
pubmed
Ritonavir and
Saquinavir directly stimulate anterior pituitary
prolactin secretion , in vitro . -
pubmed
An association between
human immunodeficiency virus type I ( HIV-1 ) protease inhibitors ( PIs ) and
galactorrhoea /
hyperprolactinemia adverse effect has recently been reported in four
HIV-1 - infected women treated with PIs (
indinavir ,
nelfinavir ,
ritonavir or
saquinavir ) . -
pubmed
This could be explained by a direct effect of
ritonavir and
saquinavir on anterior pituitary
prolactin (
PRL ) release , and/or an indirect effect of PIs on the secretion of hypothalamic
dopamine , which is the main
PRL inhibitory factor . -
pubmed
We found that both
ritonavir and
saquinavir are able to significantly stimulate
PRL secretion , with
saquinavir slightly more effective than
ritonavir . -
pubmed
On the contrary , both
protease inhibitors do not modify either basal or depolarization - induced
dopamine release . -
pubmed
[ Influence of
atrazine on the
tyrosine environment of
catalase ] . -
pubmed
By the UV difference spectrum and fluorescence spectrum technique , the influence of
atrazine on
tyrosine environment of
catalase was studied . -
pubmed
UV difference spectrum and fluorescence spectrum of
atrazine - catalase were red shift that maybe caused by effect of
hydrogen bonding . -
pubmed
The results indicated that the
tyrosine residues of
catalase reside in hydrophilic core , and there is
hydrogen bonding between
atrazine and
catalase probably . -
pubmed
Atrazine has little effect on
tyrosine environment of
catalase . -
pubmed
The inhibitor
LY294002 was able to completely abolish survival mediated by
KL , whereas
IL-3 and
FL were only partially affected . -
pubmed
The addition of
LY294002 did not affect the expression of
Bcl-2 and
Bcl - XL , and thus , we conclude that expression of antiapoptotic Bcl-2 family member genes is not dependent on
PI-3 kinase activity . -
pubmed
Finally , I review recent evidence that the soluble
N - ethylmaleimide - sensitive factor attachment protein receptor (
SNARE ) complex , formed between proteins residing in the vesicle and the plasma membrane , is involved in formation and stabilization of the readily releasable vesicle pool , whereas
synaptotagmin , a
Ca2 + - and
phospholipid - binding vesicular protein , is involved in setting the
Ca2 + dependence of the fusion process itself . -
pubmed
MPS IVA patients had an elevation of urine and plasma keratan
sulfate (
KS ) concentrations compared with those of the age - matched control . -
pubmed
OBJECTIVE : To study the influence of
Cefaclor and
Prednisonum to the expression of
cell adhesion molecule in experimental
otitis media with
effusion (
OME ) . -
pubmed
Cefaclor and
Prednisonum may improve
otitis media with
effusion , which may related to the decreasing expression of
Mac-1 and
L - Selectin . -
pubmed
AL-6556 and
AL-6598 had an affinity ( Ki ) of 2.66 - 4.43 microM for
DP receptors but a much lower affinity ( K(i)s = 38 - 103 microM ) for
EP3 ,
FP ,
IP , and
TP receptors ( n = 3 - 5 ) . -
pubmed
In addition ,
AL-6556 and
AL-6598 exhibited K(i)s & gt ; 100 microM for 19
nonprostanoid receptors . -
pubmed
The
DP antagonist ,
BWA868C , effectively antagonized the effects of
AL-6556 with a high potency ( IC50 = 22.8 + /- 3.9 nM ; n = 3 ) . -
pubmed
EP2 receptors labeled with
[ 3H ] PGE2 were concentrated in the LCM , CM , RET , and iris . -
pubmed
In conclusion ,
AL-6598 and
AL-6556 are relatively
DP - receptor - selective PGs with full agonist activity at the DP and partial agonist activity at the
EP2 receptor . -
pubmed
Neutrophil activation was influenced neither by 0.125 %
human serum albumin (
HSA ) alone nor by vesicles of
F6H8 or
silicone oil prepared with 0.125 %
HSA . -
pubmed
From our previous results , inhibition of various
polymerases by
8 - OH - G increases in the following order :
Poleta &
lt ;
Polkappa &
lt ;
Polbeta &
lt ; Polalpha , showing that major replicative and repair
polymerases are more sensitive to this lesion than enzymes belonging to the Y - family . -
pubmed
In the direct mutagenesis experiments ,
Polkappa was found to be more mutagenic than Poleta studied previously : it inserted
dAMP more efficiently than
dCMP opposite
8 - OH - G. -
pubmed
Two adjacent
8 - OH - G residues in codon 12 inhibited
Polkappa moderately and induced misincorporation of
dAMP . -
pubmed
A 78 -
amino acid insertion ( L78 ) is found in the low - affinity type ( L - form ) of
starch phosphorylase (
L - SP ,
EC 2.4.1.1 ) . -
pubmed
However ,
H - SP ( the high - affinity isomer of
SP lacking the L78 insertion ) and the proteolytic modified
L - SP , which lost its L78 fragment , could not be phosphorylated . -
pubmed
Furthermore , using L78P mutants by site - directed mutagenesis at
Ser residues on L78 , we demonstrate that only one
Ser residue on L78 is phosphorylated by the
kinase . -
pubmed
These results imply that this
kinase is specific to
L - SP , or more precisely , to the L78 insertion . -
pubmed
Chemical integrity of the lesion containing
oligonucleotides was probed using
phosphodiesterase lability . -
pubmed
We have also studied whether
selenomethionine can modify the effect of overexpression of
alpha - synuclein during differentiation of
NB cells . -
pubmed
We have utilized
RO20 - 1724 , an inhibitor of
cyclic nucleotide phosphodiesterase , and
prostaglandin A1 (
PGA1 ) , a stimulator of
adenylate cyclase , or
RO20 - 1724 and
dibutyryl cAMP to induce terminal differentiation in over 95 % of the cell population by elevating the intracellular levels of
cAMP in
NB cells . -
pubmed
The viability of cells was determined by
MTT assay and
LDH leakage assay , and the degeneration was documented by photomicrographs . -
pubmed
When
selenomethionine was added to
NB cells overexpressing
alpha - synuclein immediately after the addition of
RO20 - 1724 and
PGA1 , the viability and degenerative changes were markedly reduced , suggesting the involvement of increased oxidative stress in the mechanism of action of
alpha - synuclein . -
pubmed
Orientation of
6 - mercaptopurine SAMs at the
silver electrode as studied by Raman mapping and in situ
SERS . -
pubmed
Stability of
6MP SAMs was observed by in situ
SERS spectroelectrochemical measurements . -
pubmed
The difference between the binding of water and
methanol is that the water molecule forms a
hydrogen bond to
O5 of
pyrroloquinoline quinone , which the
methanol , by virtue of its
methyl group , does not . -
pubmed
The results support the proposal that
aspartate rather than
glutamate is the catalytically active base for a
hydride transfer mechanism in
quinoprotein alcohol dehydrogenases . -
pubmed
1,4 - Dihydroisonicotinic acid derivatives (
1,4 - DHINA ) are compounds closely related to derivatives of
1,4 - dihydropyridine , a well - known
calcium channel antagonists . -
pubmed
Third - generation
cephalosporins and beta - lactam /
beta - lactamase inhibitor antibacterials as well as
clindamycin or
metronidazole are often required to provide broad - spectrum coverage for
polymicrobial infections . -
pubmed
However , unlike the green tissues , white tissues had no detectable level of
galactinol synthase activity and a low level of
sucrose phosphate synthase indicating that
stachyose and possibly
sucrose present in white tissues may have originated in green tissues . -
pubmed
Both tissues contained high levels of
invertase ,
sucrose synthase and
alpha - galactosidase and extensively metabolized exogenously supplied
( 14 ) C - sugars . -
pubmed
Putative raft markers were found to be dynamically compartmented within tens of milliseconds into small microdomains ( O & lt;120 nm ) that are sensitive to the
cholesterol and
sphingomyelin levels , whereas
actin - based cytoskeleton barriers are responsible for the confinement of the
transferrin receptor protein . -
pubmed
We measured lesion size , macrophage , smooth muscle cell , and
collagen accumulation at the aortic
sinus , and expression of genes that regulate
cholesterol transport and
inflammation . -
pubmed
In peritoneal macrophages ,
PPAR - gamma and
ABCA1 expression was also reduced by 50 % and 70 % , respectively , as was
cholesterol efflux , by 50 % . -
pubmed
We suggest this protective role is mediated by
PPAR - gamma and
ABCA1 , molecules that affect
cholesterol homeostasis and
inflammation . -
pubmed
Three open reading frames encoding peptides larger than 50
amino acids were also identified :
repB , encoding a
replicase of 315
amino acids , a transcriptionally coupled gene (
orfX - like ) , similar to the
orfX of some theta - replicating lactococcal plasmids , and
copG - like in the complementary strand , which showed a conserved domain present in proteins of the
CopG family . -
pubmed
Furthermore , our data indicate that mitoK(
ATP ) , in addition to
UCPs , may be involved in the control of energy metabolism and
body weight . -
pubmed
Losartan preserves integrity of cardiac gap junctions and
PGC-1 alpha gene expression and prevents cellular apoptosis in remote area of left ventricular myocardium following
acute myocardial infarction . -
pubmed
This study tests the hypothesis that
peroxisome proliferator activated receptor - gamma coactivator 1alpha (
PGC-1alpha ) and the integrity of gap junctions ( GJs ) were suppressed and the number of apoptotic bodies was increased in remote viable areas of left ventricle following
acute myocardial infarction (
AMI ) , which can be reversed by
losartan therapy . -
pubmed
These compounds have been characterized by elemental analyses , FTIR , ESI -
MS , and multinuclear (
1H ,
19F , and
31P ) NMR spectroscopy . -
pubmed
Several recent studies have confirmed the efficacy and safety of both inhaled
corticosteroids and
leukotriene receptor antagonists in the management of early childhood
asthma . -
pubmed
Based on the current body of evidence , there is rationale for further investigation of these management strategies , including direct comparisons between inhaled
corticosteroids and
leukotriene receptor antagonists , as well as the role of long - acting beta - agonists , potentially targeting the subpopulations of early childhood with
wheezing who are at highest risk for persistence of
asthma symptoms . -
pubmed
The results indicate that
psychotic disorders are associated with elevated baseline and challenge - induced
HPA activity , that antipsychotic medications reduce
HPA activation , and that agents that augment stress hormone (
cortisol ) release exacerbate
psychotic symptoms . -
pubmed
The football match induced decreased neutrophil
vitamin E levels and
catalase and
glutathione peroxidase activities but increased
glutathione reductase activity . -
pubmed
These enzymes are characterized by the presence of a conserved
helicase domain with specific
helicases motifs whose
amino acid sequence allows the differentiation between DEAD and DExH - box RNA
helicase families . -
pubmed
The consideration of the observed thermodynamic parameters suggested that the increased rigidity of the
MOR - modified
TFO in the free state relative to the corresponding unmodified
TFO may enable the significant increase in the binding constant . -
pubmed
Analysis of
melamine in milk powder by CZE using UV detection and hyphenation with ESI quadrupole /
TOF MS detection . -
pubmed
An easy to employ method for the determination of
melamine in milk powder samples using CZE with either UV or
MS detection is presented . -
pubmed
Before analysis , milk powder samples were dissolved in water , de - fatted with
dichloromethane and diluted with
ACN to precipitate the
protein , filtrated and injected without further pretreatment . -
pubmed
The developed CZE method allowed the determination of
melamine in milk powder down to a level of 0.50 mg / kg using UV detection and 0.25 mg / kg when ESI quadrupole /
TOF MS was employed for detection . -
pubmed
The inner diameter of vessels was recorded by a computerized diameter tracking system and the effects of
K (+ ) channels on the development of vascular myogenic tone was tested by application of various
K (+ ) channel inhibitors . -
pubmed
Characterization of the nebulized
tacrolimus dispersion for nebulization showed a fine particle fraction (
FPF ) of 46.1 % and a mass median aerodynamic diameter ( MMAD ) of 4.06 microm . -
pubmed
A
20 - amino - acid tandem repeat that forms the core protein of
MUC1 is overexpressed and aberrantly glycosylated in the majority of
epithelial tumors . -
pubmed
AS1402 is a potent inducer of antibody - dependent cellular cytotoxicity ( ADCC ) , specifically against
MUC1 - expressing
tumor cells . -
pubmed
Letter by Choi et al regarding article , " Effects of the selective
estrogen receptor modulator
raloxifene on coronary outcomes in the
raloxifene use for the heart trial : results of subgroup analyses by age and other factors " . -
pubmed
Consistent with mediation by
D ( 1 )- type receptors ,
SCH-23390 [
R -(+)- 7 - chloro-8 - hydroxy-3 - methyl-1 - phenyl-2,3,4,5 - tetrahydro-1H-3 - benzazepine ] reversed the effects of
dopamine on spikes . -
pubmed
Three - dimensional ( 3D ) visualization of
endolymphatic hydrops after intratympanic injection of
Gd - DTPA : optimization of a 3D - real inversion - recovery turbo spin - echo ( TSE ) sequence and application of a 32 - channel head
coil at 3T. -
pubmed
PURPOSE : To enable volume visualization of
endolymphatic hydrops of
Meniere ' s disease via a volume rendering ( VR ) technique , a three - dimensional ( 3D ) inversion - recovery ( IR ) sequence with real reconstruction ( 3D - real IR ) sequence after intratympanic injection of
Gd - DTPA was optimized for higher spatial resolution using a 32 - channel head
coil at 3T. -
pubmed
Examining precursors from the purest white adipose tissue depot ( epididymal ) , we report here that chronic treatment with the
peroxisome proliferator - activated receptor gamma agonist
rosiglitazone promotes not only the expression of
PGC-1alpha and mitochondriogenesis in these cells but also a
norepinephrine - augmentable
UCP1 gene expression in a significant subset of the cells , providing these cells with a genuine thermogenic capacity . -
pubmed
DNase activity was measured by
DNase agar clearance with and without
Methyl Green . -
pubmed
Neon shows continuous distribution throughout the channel pore in
AQP1 as is the case of water , although the
PMF of
Ne at the selective filter (
SF ) region is higher than that of water , indicating that the stability of molecules in the channel is determined not only by their size , but also by the charge distribution . -
pubmed
The ligand molecules ,
CO ( 2 ) ,
NO ,
urea , and
glycerol , have a large barrier in
PMF at the
SF region in
AQP1 , indicating that the channel is not permeable by those ligands . -
pubmed
Therefore , our theory predicts that a
NH ( 3 ) molecule can be permeated through the
AQP1 channel . -
pubmed
In
GlpF , all the ligands have negative
PMF throughout the channel pore except for
glycerol , which has a small barrier at the
SF area , approximately 2.1 kJ / mol . -
pubmed
Also ,
high - density lipoprotein (
HDL )
cholesterol in males and
triglyceride in females was significantly correlated with the IPSS total score . -
pubmed
The gene fused to
NUP98 (
nucleoporin 98 ) was detected by comparative genomic hybridization array as the
retinoid acid receptor gamma gene (
RARG ) . -
pubmed
Glutamate released from the presynaptic component activates postsynaptic
glutamate receptors that are anchored in the postsynaptic density ( PSD ) and induces subsequent molecular events in the postsynaptic cytoplasm . -
pubmed
Liver marker enzymes such as
serum alanine aminotransferase (
ALT ) and
aspartate aminotransferase (
AST ) and
serum albumin (
ALB ) ,
total protein (
TP ) , total
bilirubin ( TB ) and direct
bilirubin ( DB ) levels and the markers for oxidative defense namely
malondialdehyde (
MDA ) ,
reduced glutathione (
GSH ) , total
thiols ( TT ) and
catalase (
CAT ) were measured . -
pubmed
Treatment with
MEAC ( 200 and 400 mg / kg ) significantly prevented the rise of
MDA and
TP levels ( P<0.01 ) , and prevented the reduction of
GSH ,
CAT and TT levels significantly compared with
PCM - treated group ( P<0.01 ) . -
pubmed
An improved Abbott ARCHITECT assay for the detection of
hepatitis B virus surface antigen (
HBsAg ) . -
pubmed
Captured antigen is then detected using anti -
HBsAg antibody conjugated with the chemiluminescent compound ,
acridinium . -
pubmed
Production of novel antioxidative
prenyl naphthalen - ols by combinational bioconversion with
dioxygenase PhnA1A2A3A4 and
prenyltransferase NphB or
SCO7190 . -
pubmed
2 -( 2,3 - Dihydroxyphenyl )- 5,7 - dihydroxy - chromen-4 - one (
2 ' , 3 '- dihydroxychrysin ) generated with another
aromatic dihydroxylating dioxygenase and subsequent
dehydrogenase was also geranylated at the C-5'-
carbon by the action of
NphB. -
pubmed
Towards the discovery of a novel class of
monoamine oxidase inhibitors : structure - property - activity and docking studies on
chromone amides . -
pubmed
This sample of
phenytoin users showed no drug related adverse reactions and
CYP2C9 allele type correlation . -
pubmed
The role of
CYP2C9 polymorphism influence on
phenytoin adverse reaction remains to be determined since some literature evidence and our data found negative results . -
pubmed
Specifically , the probe consists of a superparamagnetic
iron oxide nanoparticle core ( IONP ) decorated with satellite
CdS :
Mn /
ZnS Qdots where the Qdots themselves are further functionalized with
STAT3 inhibitor ( an anti -
cancer agent ) ,
vitamin folate ( as targeting motif ) and
m - polyethylene glycol (
mPEG , a hydrophilic dispersing agent ) . -
pubmed
The Qdot luminescence is quenched in this nanocomposite probe ( " OFF " state ) due to combined electron / energy transfer mediated quenching processes involving IONP ,
folate and
STAT3 agents . -
pubmed
METHOD : Hepatic function in the immunological
liver injury model was evaluated by assessing the levels of
ALT in plasma , and the content of
MDA , ORAC ,
NO and
iNOS mRNA in liver tissues . -
pubmed
RECENT FINDINGS : The gene
SLC2A9 encodes for
GLUT9 , an important proximal tubule transporter of
uric acid . -
pubmed
Genetic studies of
urate transportation and of
uromodulin - related
nephropathy emphasize the pivotal importance of the proximal tubule in
uric acid homeostasis . -
pubmed
SUMMARY : Renal tubular handling of
uric acid is dependent on tubular transporters , one of which is
GLUT9 . -
pubmed
Mutations of its gene
SLC2A9 are associated with aberrations of
uric acid disposal . -
pubmed
OBJECTIVE : To perform a retrospective analysis of
UFT and oral
leucovorin plus
PSK combination adjuvant chemotherapy for stage III
colon cancer in order to evaluate both treatment efficacy and toxicity . -
pubmed
As well as lower meristem cell activity , the second reason for the
Se - induced growth hindrance is the hormonal imbalance , since the in situ expression of the
auxin - responsive
DR5::
GUS , and consequently the
auxin levels , significantly decreased , while that of the cytokinin - inducible
ARR5::
GUS and the
ethylene biosynthetic
ACS8::
GUS increased . -
pubmed
High levels of
NO in
gsnor1 - 3 , lead to
selenite tolerance , while low
NO production in
nia1nia2 resulted in
selenite sensitivity . -
pubmed
Dyslipidemia , and especially
atherogenic dyslipidemia , a combination of
small low - density lipoproteins cholesterol (
LDL - C ) , decreased
high - density lipoprotein cholesterol (
HDL - C ) and increased
triglyceride (
TG ) concentrations , represents a major cardiovascular ( CV ) risk factor . -
pubmed
This study aims to determine whether hormone therapy (
HT ) for 1 year can modulate adrenal
androgen production . -
pubmed
A 34 - point
AIS -
APS was developed from the set of independent predictors including age , history of
atrial fibrillation ,
congestive heart failure ,
chronic obstructive pulmonary disease and current smoking , prestroke dependence ,
dysphagia , admission National Institutes of Health
Stroke Scale score , Glasgow Coma Scale score ,
stroke subtype ( Oxfordshire ) , and blood
glucose . -
pubmed
Plasma total
cholesterol concentrations were significantly lower in the XD group than in the
CD group ( p & lt ; 0.05 ) . -
pubmed
Urinary amounts of
equol were significantly higher in the XD group than in the
CD group ( p & lt ; 0.05 ) . -
pubmed
MGM-16 exhibited a high affinity for
mu - and delta - opioid receptors , with K(i ) values of 2.1 and 7.0 nM , respectively . -
pubmed
Use of
CO as metabolic fuel for microbes relies on enzymes like
carbon monoxide dehydrogenase (
CODH ) and
acetyl - CoA synthase (
ACS ) , which catalyze conversions resembling processes that eventually initiated the dawn of life . -
pubmed
CODHs catalyze the ( reversible ) oxidation of
CO with water to
CO2 and come in two different flavors with unprecedented active site architectures . -
pubmed
Ni- and
Fe - containing CODHs are frequently associated with
ACS , where the CODH component reduces
CO2 to
CO and
ACS condenses
CO with a
methyl group and
CoA to
acetyl - CoA.Our current state of knowledge on how the three enzymes catalyze these reactions will be summarized and the different strategies of
CODHs to achieve the same task within different active site architectures compared . -
pubmed
PLD generates
phosphatidic acid by catalyzing the hydrolysis of
phosphatidylcholine (
PtdCho ) and in some systems this
PtdOH is de - phosphorylated to generate
DAG . -
pubmed
In contrast ,
DGK catalyzes the phosphorylation of
DAG thereby converting it into
PtdOH . -
pubmed
While both
enzymes are poised to regulate the levels of
DAG and
PtdOH , therefore , they both lead to the generation of
PtdOH and could have opposite effects on
DAG levels . -
pubmed
In this report , we review the recent understanding of the roles
PLDs ,
DGKs , and
DAG lipases , as well as
sphingolipids and
cholesterol play in synaptic vesicle cycling . -
pubmed
Cell cycle analysis suggested that
CUR micelles induced apoptosis and enhanced
G2 / M arrest . -
pubmed
MAIN MEASURES : Use of advanced imaging (
CT / MRI ) ,
opioids /
barbiturates , and referrals to other physicians ( guideline - discordant indicators ) , as well as counseling on lifestyle modifications and use of preventive medications including
verapamil ,
topiramate ,
amitriptyline , or
propranolol ( guideline - concordant during study period ) . -
pubmed
Only herd A and B
strains harboured the genes for
beta - lactams resistance ; the
leukocidin D / E gene , a type I site - specific
deoxyribonuclease subunit ,
3rd locus gene and
serin - protease A and B were carried by all
strains , but not by V329 , while
serin - protease E was absent in V329 and in another isolate . -
pubmed
Here it is described how photon - in photon - out hard X - ray spectroscopy techniques can be applied to study the electronic structure and its dynamics of
transition metal systems with ultra -
bright and ultra - short FEL X - ray
pulses . -
pubmed
Postoperative
CD36 values were significantly correlated with total
aneurysm volume ( p = 0.05 ) and were higher in endografts made from
polyester ( p = 0.01 ) . -
pubmed
Superoxide scavengers were not radioprotective in this paradigm , indicating the mechanisms for both loss of clonogenicity and
PB1 radioprotection are independent of
superoxide signaling . -
pubmed
In contrast 1 ,
25 ( OH ) 2D3 had no significant effect upon the expression of either
TRPV 5 or 6 at either the protein or the mRNA level . -
pubmed
Para - amino acetanilide biodegradation was assessed using spectrophotometry and confirmed by gas chromatography - mass spectroscopy (
GC -
MS ) . -
pubmed
According to
GC -
MS results ,
TBZ3 degrades
PAA to
benzene ,
hexyl butanoate ,
3 - methyl-1 - heptanol and
hexyl hexanoate . -
pubmed
CONCLUSIONS :
LMWH bridging therapy significantly increases the risk of pacemaker pocket
hematoma and
hemorrhage , and the pacemaker implantation may be safer after
DES implantation 6 months . -
pubmed
RESULTS :
Cuprizone caused loss of
myelin basic protein (
MBP ) immunofluorescence in the CC suggesting
demyelination . -
pubmed
Cuprizone increased the density of
CD45+/
Iba1 + microglial cells and also increased
Gal-3 expression in the CC . -
pubmed
Surprisingly , the number of
Gal-3 + and CD45 + cells decreased in the SVZ after
cuprizone , suggesting
inflammation was selectively reduced therein . -
pubmed
Inflammation can regulate SVZ proliferation and indeed the number of
phosphohistone H3 + ( PHi3 + ) cells decreased in the SVZ but increased in the CC in both genotypes after
cuprizone treatment . -
pubmed
CONCLUSIONS : This study reveals a uniquely regulated inflammatory response in the SVZ and shows that
Gal-3 participates in remyelination in the
cuprizone model . -
pubmed
Here , we report on the hybrid hole transport materials
4,4 '- bis -( carbazole-9 - yl ) biphenyl (
CBP ) or
poly - N - vinylcarbazole (
PVK ) doped into
poly ( 4 - butyl - phenyl - diphenyl - amine ) (
Poly - TPD ) as the hybrid hole transport layer (
HTL ) to tailor the energy band alignment between hole injection layer ( HIL ) and quantum dot ( QD ) light emitting layer in order to realize efficient quantum dot light emitting diodes ( QLEDs ) in all solution - processed fabrication . -
pubmed
Compared to the pristine
Poly - TPD based device , it is found that the electroluminescence ( EL ) performance of QLEDs can be significantly improved by 1.5 fold via addition of
CBP into Poly - TPD , which can be attributed to the lowered highest occupied molecular orbital ( HOMO ) level of
Poly - TPD to reduce the energy barrier between
HTL and valance band ( VB ) of QDs . -
pubmed
The interactions between DNA and
HMGB1 are defined by
hydrogen bonds and van der Waals contacts . -
pubmed
Cytochrome P450 - 2D6 Genotype Definition May Improve Therapy for Paroxysmal
Atrial Fibrillation A Case of
Syncope Following " Pill - in - the - Pocket "
Quinidine plus
Propafenone . -
pubmed
The results demonstrated that some
flavonoids including
scutellarein ,
luteolin ,
baicalein ,
quercetin and
scutellarin displayed strong to moderate inhibitory effects against
EcGUS , with the IC50 values ranging from 5.76 muM to 29.64 muM , while
isoflavones and
dihydroflavones displayed weak inhibitory effects against
EcGUS . -
pubmed
Further investigation on inhibition kinetics revealed that
scutellarein and
luteolin functioned as potent competitive inhibitors against
EcGUS - mediated
PNPG hydrolysis , with the Ki values less than 3.0 muM. -
pubmed
Molecular docking simulations demonstrated that
scutellarein and
luteolin could be well - docked into the catalytic site of
EcGUS , while the binding areas of these two natural inhibitors on
EcGUS were highly overlapped with that of
PNPG on
EcGUS . -
pubmed
Additionally , the structure - inhibition relationships of natural
flavonoids against
EcGUS are also summarized , which will be very helpful for the medicinal chemists to design and develop more potent
flavonoid - type inhibitors against
EcGUS . -
pubmed
We found that these
plastids have lost
isopentenyl pyrophosphate biosynthesis and
ribulose-1,5 - bisphosphate carboxylase / oxygenase - based
carbon fixation but have retained various proteins for other metabolic pathways , including
amino acid biosynthesis , and a portion of the Calvin - Benson cycle comprised only of glycolysis / gluconeogenesis and the reductive
pentose phosphate pathway (
rPPP ) . -
pubmed
NIES-3581 and the presence / absence of
plastid sugar phosphate transporters , we propose that plastid proteins for glycolysis , gluconeogenesis , and
rPPP are retained even after the loss of photosynthesis because they feed indispensable substrates to the
amino acid biosynthesis pathways of the plastid . -
pubmed
Given the correlated retention of the enzymes for plastid glycolysis , gluconeogenesis , and
rPPP and those for plastid
amino acid biosynthesis pathways in distantly related nonphotosynthetic plastids and cyanobacteria , we suggest that this substrate - level link with plastid
amino acid biosynthesis is a key constraint against loss of the plastid glycolysis / gluconeogenesis and
rPPP proteins in multiple independent lineages of nonphotosynthetic algae / plants . -
pubmed
The increase in
Runx2 and
ALP activity was accompanied by the activation of
calcium / calmodulin - dependent protein kinase type II (
CaMK II ) and
extracellular signal - regulated kinases 1 / 2 (
ERK1 / 2 ) , which was completely abolished by treatment with
KN93 and
U0126 , respectively . -
pubmed
Mannosylcalix [ n ] arenes as multivalent ligands for
DC - SIGN . -
pubmed
DC - SIGN is involved in the recognition process in the form of a tetramer and the multiple exposition of
carbohydrate recognition sites ( CRSs ) is amplified by the formation on the DCs membrane of patches of tetramers . -
pubmed
We herein prepared four
mannosylated calix [ n ] arenes ( 1a - d ) and tested them by Surface Plasmon Resonance (
SPR ) competition assays as inhibitors of the binding between
DC - SIGN and a mannosylated
BSA used as model of
HIV gp120 . -
pubmed
Chronic
macrolide treatment could possibly select resistant
NTM strains and so could make the treatment clearly more difficult as
macrolides are the cornerstone
NTM treatment . -
pubmed
Polyvinylpyrrolidone coated
silver nanoparticles (
PVP - AgNPs ) were applied at different concentrations to reduce total
nitrogen (
TN ) losses and the mechanisms of
nitrogen bio - transformation were investigated in terms of the
nitrogen functional enzymes and genes . -
pubmed
In this regard , glial cells express
cannabinoid receptors and synthesize
endocannabinoids , and cannabinoid drugs affect some functions of these cells that can be implicated in
schizophrenia pathobiology . -
pubmed
Acetyl - CoA promotes
glioblastoma cell adhesion and migration through
Ca2 + -
NFAT signaling . -
pubmed
However , the specific molecular mechanisms through which
acetyl - CoA production
impacts gene expression and its functional roles in promoting malignant phenotypes are poorly understood . -
pubmed
Here , using
histone H3 Lys27 acetylation ( H3K27ac ) ChIP - seq ( chromatin immunoprecipitation [ ChIP ] coupled with next - generation sequencing ) with normalization to an exogenous reference genome ( ChIP - Rx ) , we found that changes in
acetyl - CoA abundance trigger site - specific regulation of H3K27ac , correlating with gene expression as opposed to uniformly modulating this mark at all genes . -
pubmed
Genes involved in
integrin signaling and cell adhesion were identified as
acetyl - CoA - responsive in
glioblastoma cells , and we demonstrate that
ATP citrate lyase (
ACLY)-dependent
acetyl - CoA production promotes cell migration and adhesion to the extracellular matrix . -
pubmed
Mechanistically , the
transcription factor NFAT1 (
nuclear factor of activated T cells 1 ) was found to mediate
acetyl - CoA - dependent gene regulation and cell adhesion . -
pubmed
This occurs through modulation of
Ca2 + signals , triggering
NFAT1 nuclear translocation when
acetyl - CoA is abundant . -
pubmed
The findings of this study thus establish that
acetyl - CoA impacts H3K27ac at specific loci , correlating with gene expression , and that expression of cell adhesion genes are driven by
acetyl - CoA in part through activation of
Ca2 + -
NFAT signaling . -
pubmed
CONCLUSIONS : For CHR females , the effect of
CAT on adult
SUI is mediated via
depression , while for males ,
CAT and its domains have mainly direct effects in maintaining
SUI . -
pubmed
Some GABAergic terminals are negatively influenced by
5 - HT , suggesting that the
hyperphagic effect of
CB1R activation could involve changes in serotonergic and GABAergic signaling in the PVN . -
pubmed
Here , we have found that the neurochemical mechanisms activated by stimulation of
CB1 receptors in the PVN involve inhibition of
5 - HT release , resulting in a decrease of serotonergic activity mediated by
5 - HT1A and
5 - HT1B receptors and inducing disinhibition of
GABA release to stimulate food intake . -
pubmed
These
lysins showed rapid killing kinetics and were active in the presence of high concentrations of salt and
urea and under pH conditions ranging from 5.0 to 10.0 . -
pubmed
Each model was evaluated for
weight gain , fat mass , fasting plasma
glucose ,
insulin and
C - peptide ,
insulin sensitivity ,
glucose tolerance , lipid profile and liver lipid deposition , blood pressure , and autonomic nervous system function . -
pubmed
Samples were divided into control ( Group I - normal saline , Group II-17 %
ethylenediaminetetraacetic acid ) and experimental ( Group III ,
IV , V , VI , VII , VIII , and IX ) groups based on the type of final rinse solution ( 5 mL ) used , that is , TA - premixed ( P ) , TA-(P)-sonic , TA-(P)-ultrasonic , 3 % TA solution , 5 % TA solution , 10 % TA solution , and 10 %
citric acid . -
pubmed
Transglutaminases (
TGs ) are
Ca2 + - and
thiol - dependent
acyl transferases and protein crosslinking enzymes . -
pubmed
Post - annotation , we randomly selected a set of hundred proteins and computed the percentage distribution of 12
amino acids which have been substantially studied for their role in thermostability . -
pubmed
Subsequently , the utility of this HIC -
MS method was demonstrated in three different applications , where a
mAb mixture , mAb molecular variants resulting from
PTMs , and a
Cys - based ADC mimic were all readily characterized in detail . -
pubmed
Also ,
vitaminD reduces the production of
inflammatory cytokines , such as
IL-2 and
interferon - gamma (
INF - gamma ) . -
pubmed
Of interest , another mechanistic approach responds to considering the inhibition of the
renin -
angiotensin -
aldosterone system ( RAAS ) , which is exacerbated in
COVID-19 infection because the virus binds to the enzyme
ACE2 , making more
angiotensinII available to cause damage . -
pubmed
VitaminD inhibits mediators of
RAAS - present in all cells of the body - and by inhibiting
ACE activity and increasing
ACE2 , it lowers
angiotensinII levels . -
pubmed
This study investigated the effect of AT pretreatment of
spectinomycin mycelial residues ( SMRs ) for promoting anaerobic biodegradability along with the reduction of antibiotic resistance genes ( ARGs ) , and thus obtained the optimal conditions of
AT pretreatment . -
pubmed
Biomethane potential ( BMP ) test was conducted to evaluate the anaerobic biodegradability of untreated and pretreated SMRs , and the fate of ARGs was tracked by quantitative
polymerase chain reaction . -
pubmed
5 - Hydroxymethyl cytosine ,
enhancer of zeste homolog 2 ,
cyclin D1 , and
programmed death - ligand 1 IHC , and
NF2 FISH are all potentially useful but need further study . -
pubmed
Emodin enhances
cisplatin sensitivity in
non - small cell lung cancer through
Pgp downregulation . -
pubmed
Significant differences are found in the digestion pattern of the two tRNAs using the
guanosine - specific
Tl nuclease . -
pubmed
In carrying out these analyses , the
tRNA molecules are labeled either by putting
32P at the 5'-end of the molecular or by adding
32P - labeled pCp at the 3'-end . -
pubmed
coli tRNA2Glu have modified
Tl nuclease digestion patterns when
pCp is added at the 3'-end of the molecule . -
pubmed
Evidence is reviewed that
5 - HT (
5 - hydroxytryptamine ,
serotonin ) acting through the
5 - HT3 receptor subtype can influence behaviour relevant to
anxiety ,
schizophrenia and
cognitive disorders , and that
5 - HT3 receptor antagonists such as
ondansetron (
CAS 116002 - 70 - 1 ) can correct behavioural disturbance in the absence of effect on normal behaviour . -
pubmed
In the active stage of
UC , mucosal
spermidine concentration was higher than in remission or in the controls , but the activities of
ornithine decarboxylase and
S - adenosylmethionine decarboxylase , rate - limiting enzymes of
polyamine biosynthesis , were lower . -
pubmed
The activity of
spermidine / spermine N1 - acetyltransferase , the rate - limiting enzyme of
polyamine degradation , was higher in the active stage of
UC than in the remission stage of
UC or in the controls . -
pubmed
Electron microscopy using peroxidase antiperoxidase reveals that
tenascin is present in nonstriated , 10 nm wide fibrils and in interstitial bodies , both of which have previously been reported to contain
fibronectin . -
pubmed
The discussion emphasizes the possible importance of
type I collagen in neural crest cell migration and compares the ultrastructural associations of the ECM
molecules present at this early embryonic stage . -
pubmed
SSAO activity towards
dopamine was present in the choroid and the retina , but not in the iris or the optic nerve . -
pubmed
The absence of detectable
SSAO activity towards
dopamine in these last two tissues contrasts with the presence of that enzyme when
benzylamine was studied as substrate . -
pubmed
Light - induced
Ca2 + release from the
Ca2 + complex of
Nitr-5 altered the FTIR spectra of sarcoplasmic reticulum vesicles and purified
Ca ( 2 +)- ATPase preparations . -
pubmed
Even though
MHC polymorphism has long been known to affect both peptide binding and recognition by the
TCR , the role of individual
amino acids of
MHC proteins in these interactions is poorly understood . -
pubmed
Differences in binding of a set of
HA307 - 319 analogues biotinylated at each residue to cells expressing
DR1 Dw1 and
DR1 Dw20 suggested that the polymorphism affected the interactions of many
peptide residues with the
class II molecule . -
pubmed
In inhibition assays ,
DR1 Dw1 and
DR4 Dw4 were shown to differ from
DR1 Dw20 and
DR4 Dw14 in their length requirements for
peptide binding . -
pubmed
The results indicated that polymorphism at residue 86 influenced the specificity and affinity of
peptide binding and affected the conformation of
peptide - DR protein complexes without completely eliminating T cell recognition . -
pubmed
Its
amino acid composition is similar to that of " tuft " proteins (
enamel matrix protein fragments remaining in the mature tissue ) . -
pubmed
Effects of nerve agent antidote and heat exposure on soldier performance in the BDU and
MOPP -
IV ensembles . -
pubmed
This study assessed the effects of nerve agent antidote (
atropine / 2 - PAM chloride versus saline placebo ) and heat - humidity ( 95 degrees F/60 % RH versus 70 degrees F/30 % RH ) on repeated performance of militarily relevant psychological tasks while wearing the battle dress uniform ( BDU ) and while wearing chemical protective clothing ( MOPP -
IV ) . -
pubmed
MOPP -
IV heat sessions could not be continued beyond 2 hours ; all tasks were impaired and subjective reactions were numerous and severe . -
pubmed
Atropine/2 -
PAM significantly shortened endurance time for heat sessions in MOPP -
IV . -
pubmed
We measured mean arterial and central venous pressure , heart rate , arterial
lactate , blood volume ( 51Cr - labeled red cells ) , hematocrit ( conductivity cell ) , macromolecular extravasation ( 125I -
HSA ) and fluid retention ( calculated from change in plasma volume ) . -
pubmed
In the presence of
alcohols the
enzyme catalyses the transfer of the substrate
acyl group to
alcohols in parallel to hydrolysis . -
pubmed
On the basis of titration with
diethyl p - nitrophenyl phosphate , a number of four active centres is determined for the tetrameric
carboxylesterase . -
pubmed
The sequence of 20
amino acid residues at the
esterase N - terminus and the
amino acid composition are reported . -
pubmed
The responses to
CCK and BBS were similar in shape , time course , and reversal potential to that induced by receptor mediated
phospholipid breakdown and that which is induced by intracellular injection of
IP3 . -
pubmed
After administration of
zimeldine in combination with
5 - HTP there was an increase in synchronized waking ( W-2 ) , followed by an increase in slow wave sleep (
SWS ) , at first SWS-1 with spindles and then highly synchronized SWS-2 . -
pubmed
Maximal responses to
milrinone and
parathyroid hormone were comparable . -
pubmed
We find similarities between
milrinone and
thyroxine actions on bone , because
prostaglandin production was crucial for the effects of both agents in calvaria but not in limb bones , and neither agent exhibited escape from
calcitonin inhibition . -
pubmed
These mutants exhibited a
Nif- phenotype in the presence of
vanadium , unlike
CA12 which was
Nif + on
vanadium - containing medium . -
pubmed
The gene in the cloned
nifH - like region is therefore apparently involved in the
vanadium dependent alternative pathway of
nitrogen fixation . -
pubmed
This gene ,
nifH2 , has been sequenced and encodes a protein of 289
amino acids that is similar to
nifH in nucleotide sequence , deduced
amino acid sequence , predicted secondary structure and hydrophobicity profile . -
pubmed
Molecular analysis of
HLA class I and class
II antigen loss mutants reveals a homozygous deletion of the DR , DQ , and part of the DP region : implications for class
II gene order . -
pubmed
DZ alpha genomic sequences were deleted as were the
DP alpha 1 and DP
beta 1 loci but the DP
beta 2 and most , if not all , of the
DP alpha 2 locus were retained . -
pubmed
In addition , the analysis of a set of DR- , DQ- , DP+ homozygous deletion mutants ( 721.82 , 721.84 , and 721.101 ) reveals a deletion breakpoint between the DQ alpha 1 / DQ
beta 1 loci and the DQ alpha 2 / DQ
beta 2 loci . -
pubmed
Among the 156
ampicillin - susceptible ,
beta - lactamase - negative isolates , synergy was detected in 13 with
ampicillin -
cefotaxime , and antagonism was detected in only 1 ; in contrast , synergy was found in only 2
strains with
ampicillin -
chloramphenicol , and antagonism was found in 15 . -
pubmed
[ Diuretic effects of
ethacrynic acid (
E.C.A. )
II . -
pubmed
Interaction of the
benzodiazepine antagonists ,
CGS 8216 and
Ro 15 - 1788 , with central
adenosine A-1 receptors . -
pubmed
Although the potent
benzodiazepine antagonist
CGS 8216 has in vitro activity indicative of an interaction with central
adenosine systems , neither it , nor the
benzodiazepine antagonist
Ro 15 - 1788 showed any great degree of interaction with central
adenosine A-1 receptors as measured by radioligand binding . -
pubmed
It is concluded that interaction of
benzodiazepine antagonists with
A-1 receptors is not a property related to their antagonist activity . -
pubmed
She was treated with the
calcium channel blocking drug
nifedipine , with significant relief of symptoms . -
pubmed
The results show no significant deterioration in renal function as determined by serum
creatinine or
creatinine clearance , as a function of years
post - uninephrectomy or age at the time of donation . -
pubmed
Uterine
ERc and
PRc , which sedimented at the 8S and 4S regions , respectively , of a 5 - 20 %
sucrose gradient , showed similar rise and decline patterns before and after parturition . -
pubmed
Ephedrine - saline nasal
wash in
allergic rhinitis . -
pubmed
BACKGROUND :
Ephedrine nasal
wash ( 0.25 % to 1 % ) was once used in the treatment of
sinusitis and other nasal and paranasal symptoms , but there are no reported controlled trials in
allergic rhinitis . -
pubmed
RESULTS : Use of
ephedrine - saline nasal
wash resulted in significant improvement in symptom scores and peak nasal inspiratory flow rates as compared with placebo ( p & lt ; or = 0.001 ) . -
pubmed
CONCLUSION : A 1 %
ephedrine - saline nasal
wash is an inexpensive and effective treatment for
allergic rhinitis . -
pubmed
After oral
PSL ( 30 mg ) , which was given at 7:00 (
AM ) on day 2 , lymphocytes and almost all lymphocyte subsets , except for
CD16+
CD57- cells , were significantly decreased ; these changes recovered between 13 and 26 hours after
PSL administration . -
pubmed
The circadian variations and PSL - induced alterations of lymphocyte subsets were relatively comparable , but
PSL administration cause a decrease in a wider range of lymphocyte subsets including relatively
corticosteroid - resistant subsets such as
CD45RA+
CD4 + cells ,
CD8 + cell and NK cell subsets. -
pubmed
BACKGROUND :
Glutaminase , the principal enzyme of
glutamine hydrolysis , breaks down
glutamine to supply energy and intermediates for cell growth and is present in high concentrations in replicating tissues such as intestinal epithelium and
malignant tumors . -
pubmed
These reductions in
message and protein translated into a 60 - 80 % decrease in functional
glutaminase - specific activity . -
pubmed
CONCLUSIONS : This
IFN - gamma - mediated decrease in
glutaminase activity may be one mechanism by which gut
glutamine metabolism is diminished as the
tumor grows and becomes the principal organ of
glutamine use . -
pubmed
BACKGROUND / AIMS : Recently we showed that doses of
cholecystokinin octapeptide (
CCK-8 ) that produce physiological plasma
CCK levels act via stimulation of afferent vagal pathway to mediate pancreatic enzyme secretion . -
pubmed
Vagotomy or perivagal application of
capsaicin , a sensory neurotoxin , abolished increases in pancreatic secretion but not plasma
CCK levels in response to diversion of bile pancreatic secretion or duodenal administration of
casein . -
pubmed
Simultaneous prodrug cleavage by nasal
carboxylesterase was also noted in the case of
hexanoate . -
pubmed
Reassortants between these
strains indicated that the
51Cr release phenotype segregates with the
M2 gene segment . -
pubmed
Biochemical studies indicated that the ISVPs ' acquisition of the capacity to induce
51Cr release followed the cleavage of the
viral M2 gene product mu 1 / mu 1C to fragments delta and phi during virion conversion to ISVP but did not directly correlate with this cleavage . -
pubmed
The chimeric subunit r1(25)r8 , containing 25
amino acid residues from subunit 1 , had the same low activity with
alkenal substrates as that displayed by
subunit 1 . -
pubmed
Mutation of
Ala-12 into
Gly in r1(25)r8 gave rise to the high
alkenal activity characteristic of
subunit 8 , showing the importance of
amino acid residue 12 for the activity . -
pubmed
The major symptoms of
Parkinson disease (
PD ) are
tremors ,
hypokinesia ,
rigidity , and
abnormal posture , caused by the degeneration of
dopamine (
DA ) neurons in the substantia nigra (
SN ) and deficiency of DA in the neostriatal DA terminals . -
pubmed
Norepinephrine ( NE ) and
serotonin ( 5 - HT ) levels in the neostriatum and
tyrosine hydroxylase and melanin pigments in the substantia nigra are also decreased , and brain cholinergic activity is increased . -
pubmed
Increased methylation can deplete DA , NE , and
5 - HT ; increase
acetylcholine ; and cause
hypokinesia and
tremors . -
pubmed
A mutant
JEM1p carrying a mutation in the highly conserved
His - Pro - Asp sequence in the J - domain could not complement either temperature - sensitive growth of the
Deltajem1 Deltascj1 double mutant or defects in karyogamy of the
Deltajem1 mutant . -
pubmed
This paper describes the process of dimer assembly of mitochondrial very - long - chain
acyl - CoA dehydrogenase (
VLCAD ) subunit . -
pubmed
Decreased immunostaining for
TGF - beta RII in
adenomas persisted for at least 8 mo after exposure to
urethane , whereas immunostaining for
TGF - beta1 , - beta2 , and - beta3 and
TGF - beta RI persisted at levels comparable to those in normal bronchioles . -
pubmed
Inhibition of
corticotropin releasing factor (
CRF)-induced
adrenocorticotropin (
ACTH ) secretion in pituitary corticotropic cells by
substance P. -
pubmed
Substance P (
SP ) is one of the three distinct peptides of
tachykinin system which possess a common spectrum of biological activities including a modulation of stress . -
pubmed
In the same way SP inhibited the
CRF - induced accumulation of
cyclic adenosine monophosphate (
cAMP ) , indicating that SP influenced the signal transduction pathway of
CRF receptor activation . -
pubmed
These experiments , as well as others assaying the strength of factor association in high - salt
sucrose gradients , lead us to conclude that
Upf1p is tightly bound to the smallest polyribosomes , but not to the 40S or 60S ribosomal subunits . -
pubmed
Using
sucrose gradient fractionation and Northern blotting , we can detect the polysomal association of a
PGK1 mRNA decay intermediate and conclude that mRNA decay commences while an mRNA is still being translated . -
pubmed
The effects of interfering with
GTP - binding proteins on the activation mechanism of
calcium release - activated
calcium current . -
pubmed
It has been suggested that , in some cell types , the link is accomplished by either a small or a heterotrimeric
GTP - binding protein , which is inhibited by
guanosine 5 '- O -( 3 - thiotriphosphate ) (
GTP [ gamma - S ] ) and , in some cases ,
pertussis toxin . -
pubmed
Putting together a physician practice group and then creating the management and administrative structure for that practice to financially succeed is one of the greatest challenges health systems
face today . -
pubmed
Erythromycin resistance mutations in ribosomal proteins
L22 and
L4 perturb the higher order structure of 23 S ribosomal RNA . -
pubmed
Thus , while
L22 and
L4 bind primarily to domain I of 23
S rRNA ,
erythromycin resistance mutations in these ribosomal proteins perturb the conformation of residues in domains
II , III and V and affect the action of antibiotics known to interact with
nucleotide residues in the
peptidyl transferase center of domain V. These results support the hypothesis that ribosomal proteins interact with rRNA at multiple sites to establish its functionally active three - dimensional structure , and suggest that these antibiotic resistance mutations act by perturbing the conformation of rRNA . -
pubmed
Alkaline proteinase and
sodium hydroxide were used before
phenol /
chloroform extraction to achieve high quality DNA from very old samples . -
pubmed
Immunohistochemical tests using
anti - macrophage complement receptor 3 (
OX42 ) or anti - monocyte - macrophage (
ED1 ) and
DiI - ac -
low - density lipoprotein (
LDL ) uptake were used to identify microglia . -
pubmed
Interaction of the
EEA1 FYVE finger with
phosphatidylinositol 3 - phosphate and early endosomes . -
pubmed
Here we have investigated the structural requirements for the interaction of the FYVE finger of the early endosome antigen
EEA1 with
PtdIns ( 3 ) P and early endosomes . -
pubmed
These results support recently proposed models for the ligand binding of the FYVE domain and indicate that
PtdIns ( 3 ) P binding is crucial for the localization and function of
EEA1 . -
pubmed
Diacylglycerol mediates the
T - cell receptor - driven
Ca ( 2 + ) influx in T cells by a novel mechanism independent of
protein kinase C activation . -
pubmed
In this work , the role of
diacylglycerol (
DAG ) as the mediator of
T - cell receptor (
TCR)-driven
Ca ( 2 + ) influx in T cells was investigated . -
pubmed
Such stimulation was blocked by
Ni ( 2 + ) , a divalent cation known to block
Ca ( 2 + ) channels . -
pubmed
Inhibition of
protein kinase C (
PKC ) by
calphostin C did not inhibit , but slightly enhanced , the
DAG - stimulated
Ca ( 2 + ) entry . -
pubmed
However , inhibition of
DAG metabolism by
DAG kinase and
lipase inhibitors enhanced the
DAG - stimulated
Ca ( 2 + ) entry . -
pubmed
DAG lipase and kinase inhibitors also enhanced the
Ca ( 2 + ) entry in T cells stimulated through
TCR / CD3 complex with
anti - CD3 antibody . -
pubmed
Calphostin C did not affect the
anti - CD3 - stimulated
Ca ( 2 + ) entry . -
pubmed
These results showed that
TCR - driven
Ca ( 2 + ) influx in T cells is mediated by
DAG through a novel mechanism(s ) independent of
PKC activation . -
pubmed
The main
quercetin conjugate was identified as
rutin (
quercetin 3 - rhamnosylglucoside ) by LC -
MS . -
pubmed
Mutational analysis of invariant
valine B12 in
insulin : implications for receptor binding . -
pubmed
An invariant residue ,
valine B12 , is part of the
insulin B - chain central alpha - helix (
B9 -
B19 ) , and its aliphatic side chain lies at the surface of the hydrophobic core of the
insulin monomer in close contact with the neighboring aromatic side chains of
phenylalanines ( B24 and B25 ) and
tyrosines ( B26 and B16 ) . -
pubmed
To investigate in detail the role of the
B12 residue in
insulin - receptor interactions , we have synthesized nine analogues bearing natural or unnatural
amino acid replacements for
valine B12 by chemical synthesis of modified
insulin B - chains and the subsequent combination of each synthetic B - chain with natural
insulin A - chain . -
pubmed
Our results indicate that
beta - branched aliphatic amino acids are generally tolerated at the
B12 position with specific steric preferences and that the receptor binding potencies of these analogues correlate with their abilities to form dimers . -
pubmed
Furthermore , the structure - activity relationships of
valine B12 are quite similar to those of
valine A3 , suggesting that
valine residues at both
A3 and
B12 contribute to the
insulin - receptor interactions in a similar manner . -
pubmed
Using immunoblotting , a more than threefold increase in immunoreactive
ROMK levels was observed in the outer medulla after
dDAVP infusion . -
pubmed
Restriction of water intake to increase
vasopressin levels also significantly increased TAL
ROMK immunolabeling and abundance in immunoblots . -
pubmed
In addition , dietary
Na (+ ) levels were varied to determine whether
ROMK abundance was also affected under other conditions known to alter
TAL transport . -
pubmed
Infusion of the
alpha ( 2 )- adrenoceptor agonist
clonidine decreased extracellular
noradrenaline in the LC as well as in the PFC . -
pubmed
Infusion of the
alpha ( 2A )- adrenoceptor antagonist
BRL44408 , or the
alpha ( 1 )- adrenoceptor agonist
cirazoline into the LC or PFC caused a similar dose - dependent increase in both structures . -
pubmed
Infusion of the
5 - HT ( 1A )- receptor agonist
flesinoxan into the LC or the PFC decreased the release of
noradrenaline in both structures . -
pubmed
Ganglioside GD1a inhibits
HGF - induced motility and scattering of
cancer cells through suppression of
tyrosine phosphorylation of
c - Met . -
pubmed
The
c - Met in the cells was autophosphorylated by stimulation with
HGF , but after treating the cells with
GD1a , the
HGF - induced autophosphorylation of
c - Met was suppressed . -
pubmed
These results suggest that
GD1a acts as a negative regulator of
c - Met in
cancer cells . -
pubmed
Demethylation treatment by
5 - aza-2 '- deoxycytidine restored
AR mRNA expression in PLS20 . -
pubmed
The measurement of
iNOS activity ,
iNOS protein formation and
NO concentration in the ischemic core and penumbra , and the determination of
infarct volume were performed at 6 , 12 , 24 and 48 h after reperfusion . -
pubmed
The time course of
iNOS protein , enzymatic activity and
NO concentration in the penumbra but not in the core matched the process of
infarct maturation . -
pubmed
Treatment with
iNOS inhibitor
aminoguanidine ( 100 mg.kg(-1 ) , i.p . ) -
pubmed
at 6 and 12 h after reperfusion inhibited
iNOS activity by 88.0 + /- 10.4 % and reduced
NO concentration by 48.5 + /- 8.3 % in the penumbra , and lessened
infarct size by 48.8 + /- 7.2 % . -
pubmed
The
iNOS activity and
NO level in the core were not affected by the administration of
aminoguanidine . -
pubmed
NTP Toxicology and Carcinogenesis Studies of
Furfural (
CAS No . -
pubmed
In particular ,
TAP has stereoselectivity for
amino acid derivative substrates , hence it is useful for the kinetic resolution of racemic mixtures of industrial chemicals . -
pubmed
The structure of
TAP in complex with a
diethyl phosphono moiety (
DEP ) identified its catalytic
triad ,
Ser10 - Asp154 -
His157 , and
oxyanion hole ,
Ser10 -
Gly44 -
Asn73 . -
pubmed
The
oxyanion hole of
TAP consists of three residues each separated from the other by more than 3.5A , implying that all of them are highly polarized when substrate bound . -
pubmed
The catalytic (
His)C(epsilon1)-H ...
O = C hydrogen bond usually plays a role in the catalytic mechanisms of most
serine hydrolases , however , there were none present in
SGNH - hydrolases . -
pubmed
Through comparison of
TAP and the three
SGNH - hydrolase structures currently known , we have identified a unique
hydrogen bond network which stabilizes the catalytic center : a newly discovered structural feature of
SGNH - hydrolases . -
pubmed
[ Inhibition of hepatic
glucose-6 - phosphatase by
fatty acids and
zinc ions ] . -
pubmed
Attenuation of renal
vasopressin V2 receptor upregulation by
bosentan , an
ETA / ETB receptor antagonist . -
pubmed
The goal of the present studies was to determine if
ET-1 is involved in upregulating the expression of
AVP V2 mRNA in the IMCD of
CM by using a mixed
ETA / ETB receptor antagonist
bosentan . -
pubmed
The effect of
bosentan in normalizing the expression of
AVP V2 and
AQP2 mRNA in the IMCD of
CM was confirmed by in situ hybridization studies . -
pubmed
Two distinct methods analyzing chromatin structure using centrifugation and antibodies to modified
histone H3 : both provide similar chromatin states of the
Rit1 /
Bcl11b gene . -
pubmed
One is ChIP assay examining the degree of enrichment with
histone H3 methylated at
lysine 9 ( H3 - mLys9 ) in chromatin and the other is H / E ( heterochromatin / euchromatin ) assay that measures a chromatin condensation state by using centrifugation . -
pubmed
Mutations were identified in homozygous
F3 progeny derived from
ENU - treated founder males . -
pubmed
Aberrant methylation of a
sodium co - transporter ,
solute carrier family 5 member 8 gene (
SLC5A8 ) , has been detected in a subset of
colorectal cancers , suggesting
SLC5A8 may also serve as a
tumor suppressor . -
pubmed
Cells of all three cell lines died by apoptosis after exposure to 1 microg / ml
nocodazole , a mitotic blocker that acts by inhibiting the polymerization of
tubulin ( & gt;25 % apoptotic cells after 24 h ) . -
pubmed
The
phosphatidylinositol 3 - kinase (
PI3 K ) signaling pathway was also involved since the
PI3 K inhibitor ,
LY294002 , abolished the
TIMP-1 - mediated growth stimulation . -
pubmed
Mechanisms of adaptations for
oxytocin neurones are reviewed , using challenge with systemic
interleukin-1beta , simulating activation of immune signaling by
infection , as a stressor of special relevance in pregnancy . -
pubmed
This lack of response is reversed by
naloxone treatment just before
interleukin-1beta administration , indicating endogenous opioid suppression of
oxytocin responses in late pregnancy . -
pubmed
Finasteride pretreatment , inhibiting
progesterone conversion to
allopregnanolone , a positive
GABA ( A ) receptor allosteric modifier , also restores an
oxytocin response to
interleukin-1beta . -
pubmed
Accordingly ,
allopregnanolone may both enhance
GABA inhibition of
oxytocin neurone responses to
interleukin-1beta , and induce opioid suppression of
noradrenaline release onto
oxytocin neurones . -
pubmed
BACKGROUND AND PURPOSE : To follow up in vitro evidence that
Delta ( 9 )- tetrahydrocannabivarin extracted from cannabis (
eDelta ( 9 )- THCV ) is a
CB ( 1 ) receptor antagonist by establishing whether synthetic
Delta ( 9 )- tetrahydrocannabivarin (
O-4394 ) and
Delta ( 8 )- tetrahydrocannabivarin (
O-4395 ) behave as
CB ( 1 ) antagonists in vivo . -
pubmed
CONCLUSIONS AND IMPLICATIONS :
O-4394 and
O-4395 exhibit similar in vitro potencies to
eDelta ( 9 )- THCV as
CB ( 1 ) receptor ligands and as antagonists of
cannabinoid receptor agonists and can antagonize
Delta ( 9 )- tetrahydrocannabinol in vivo . -
pubmed
In the
FTY720 - treated groups ,
MAdCAM-1 expression on high endothelial venules ( HEVs ) in Peyer 's patches ( PPs ) was upregulated and its expression on endothelial cells of vessels in the lamina propria was downregulated in comparison with the allograft group without
FTY720 . -
pubmed
Vitamin E supplementation enhances
hemoglobin and
erythropoietin levels in mildly
anemic adults . -
pubmed
In this paper , we test this model by creating procyclic and bloodstream form null mutants of
TbALG3 , the gene that encodes the
alpha - mannosyltransferase that converts
Man5GlcNAc2 - PP - Dol to
Man6GlcNAc2 - PP - Dol . -
pubmed
However , both forms display aberrant N - glycosylation of their major surface
glycoproteins ,
procylcin , and variant surface glycoprotein , respectively . -
pubmed
Specifically ,
procyclin and variant surface glycoprotein N - glycosylation sites that are modified with
Man9GlcNAc2 and processed no further than
Man5GlcNAc2 in the wild type are glycosylated less efficiently but processed to complex structures in the mutant . -
pubmed
Moreover , mutation of the predicted catalytic
cysteine led to the same level of attenuation as a null mutant , suggesting that the
G6 protein has enzymatic activity that is important in vivo . -
pubmed
This treatment was also more effective than that with
Se or
Zn alone in reversing
Cd - induced decrease in serum T4 level and
Cd - induced increase in serum
TSH level . -
pubmed
The median lethal dose ( LD50 ) of
raddeanin A lavage was 1.1 g / kg and that of
raddeanin A injection was
16.1 mg / kg . -
pubmed
With Avicel as
carbon source the transcript level of
cel5A continuously increased until the 10th hour of incubation and then a sharp decrease was observed , whereas
cel5B presented a slow constitutive expression on this substrate . -
pubmed
Somatolactin is thought to be involved in a wide range of physiological actions , including reproduction , stress response , the regulation of
Ca ( 2 + ) and acid - base balance , growth , metabolism , and immune response . -
pubmed
Sequence analysis of purified
SL revealed that the prehormone is composed of a signal peptide of 23
amino acids and a mature protein of 207
amino acids , which has a possible N - glycosylation site at position 121 and seven
Cys residues . -
pubmed
The objective of this in silico study is to elucidate the pharmacokinetic origins of incomplete viral suppression , exemplified for
zidovudine (
AZT ) as a representative of the key class of
nucleoside reverse transcriptase inhibitors ( NRTIs ) . -
pubmed
Inhibition of the
cannabinoid type-1 receptor ameliorates the effects of
endocannabinoids on food intake and energy metabolism ; lipogenesis is inhibited , lipolysis ,
fatty acid oxidation and
glucose uptake increase . -
pubmed
At discharge , only serum
sodium , log - transformed
BNP , IVC size , and collapsibility were statistically significant predictors of readmission . -
pubmed
Moreover , by means of a sensitive electrochemiluminescent immunoassay employing Abeta C - terminal specific antibodies , mature oligodendrocytes are shown to secrete the 40 and 42
amino acid Abeta species (
Abeta40 and
Abeta42 ) . -
pubmed
The addition of
PrP(Sc ) or
PrP82 - 146 caused a dose - dependent reduction in the
synaptophysin content of
PrP wildtype neurones indicative of
synapse damage . -
pubmed
Pre - treatment of cortical or hippocampal neurones with
docosahexaenoic (
DHA ) or
eicosapentaenoic acids (
EPA ) protected neurones against the loss of
synaptophysin induced by
PrP82 - 146 or
PrP(Sc ) . -
pubmed
This effect of
DHA and
EPA was selective as they did not alter the loss of
synaptophysin induced by a snakevenom neurotoxin . -
pubmed
Promoter
histone H3 lysine 9 di - methylation is associated with DNA methylation and aberrant expression of
p16 in
gastric cancer cells . -
pubmed
Accordingly , we chose
p16 to clarify whether there is a correlation among
histone H3 lysine 9 ( H3 - K9 ) di - methylation ,
H3 - K9 acetylation , DNA methylation and
p16 expression in human
gastric cancer . -
pubmed
We investigated
p16 promoter DNA methylation status ,
p16 mRNA levels , regional and global levels of di - methyl - H3 - K9 and
acetyl - H3 - K9 in four groups : i )
5 - Aza - dC , ii )
TSA , iii ) the combination of
5 - Aza - dC and
TSA and iv ) control group with no treatments . -
pubmed
Treatment with
TSA , increased
H3 - K9 acetylation at the hypermethylated promoter , but did not affect
H3 - K9 di - methylation or
p16 expression . -
pubmed
By contrast , treatment with
5 - Aza - dC , reduced
H3 - K9 di - methylation , increased
H3 - K9 acetylation at the hypermethylated promoter and reactivated the expression of
p16 . -
pubmed
In addition ,
5 - Aza - dC and the combined treatment did not result in global alteration of
H3 - K9 di - methylation . -
pubmed
In addition to its effect on DNA methylation ,
5 - Aza - dC can act at histone modification levels to reactivate
p16 expression in region - specific and DNA methylation - dependent manner . -
pubmed
Presumptive
Estrogen Target Neurons Express mRNAs for both the
Neurotrophins and
Neurotrophin Receptors : A Basis for Potential Developmental Interactions of
Estrogen with the
Neurotrophins . -
pubmed
Estrogen and the
neurotrophins regulate development , survival , and plasticity of the nervous system . -
pubmed
These results suggest a potential for interactions between
estrogen and the
neurotrophins , including possible
estrogen - stimulated ,
neurotrophin - mediated autocrine mechanisms that may regulate neuronal differentiation and survival during development . -
pubmed
A self - produced radioimmunoassay and relevant kits were used to detect the protein level of
orexin - A in the plasma and the hypothalamus , serum
glucose , total anti - oxidation capacity and
alanine transaminase , HE staining and immunohistochemistry were used to investigate the pathological variation and protein expression of
orexin - A in the liver , while RT - PCR was applied to observe mRNA expression of
orexin - A in the hypothalamus and the liver . -
pubmed
There was significant and positive linear correlation between the plasma
orexin - A and serum
glucose and total anti - oxidation capacity ; serum
alanine transaminase in each reperfusion group was significantly higher , and
liver damage was significantly alleviated at the later phase of the
injury . -
pubmed
Then , by combining the molecular weight of the so - obtained homopolymers , as measured by
MS , with the relative molar proportion of the
MMA and
MMA units , as given by
( 1 ) H NMR spectrum , one can straightforwardly estimate the molecular weight of the initial copolymer . -
pubmed
Hb Perpignan [ beta136(H14)Gly-->
Ser ] , a silent variant associated with normal hematology . -
pubmed
Inhibition of
interleukin-1beta converting enzyme (
ICE or
caspase 1 ) by
aspartyl acyloxyalkyl ketones and
aspartyl amidooxyalkyl ketones . -
pubmed
Novel screening cascade identifies
MKK4 as key
kinase regulating
Tau phosphorylation at
Ser422 . -
pubmed
Phosphorylation of
Tau at
serine 422 promotes
Tau aggregation . -
pubmed
An overexpression of
netrin2 at these two developmental stages suggests a novel role for this gene in regulating axonal density specific to
Pb neurotoxicity . -
pubmed
Ceramidases are key enzymes that control this rheostat by hydrolyzing
ceramide to produce
sphingosine and may also confer resistance to drugs and radiation . -
pubmed
In this study , we identify a novel
ceramidase inhibitor (
Ceranib-1 ) by screening a small molecule library and describe the synthesis of a more potent analogue (
Ceranib-2 ) . -
pubmed
In a cell - based assay , both compounds were found to inhibit cellular
ceramidase activity toward an exogenous
ceramide analogue , induce the accumulation of multiple
ceramide species , decrease levels of
sphingosine and
S1P , inhibit the proliferation of cells alone and in combination with
paclitaxel , and induce cell - cycle arrest and cell
death . -
pubmed
AFB ( 1 ) activation leads to DNA adduction and mutagenesis , with a specific mutation at codon 249 in
TP53 ( p. R249S ) . -
pubmed
Conversely ,
HBV infection did not significantly modify
AFB ( 1 )-DNA adduct formation or repair as assessed by immunodot - blot assay , and the induction of
p53 in response to
AFB ( 1 ) was similar in infected and non - infected HepaRG cells . -
pubmed
The iAbeta5p beta - breaker peptide regulates the
Abeta(25 - 35 ) interaction with lipid bilayers through a
cholesterol - mediated mechanism . -
pubmed
In this work , we focused on the effect of the beta - sheet breaker peptide
acetyl - LPFFD - amide ,
iAbeta5p , on the interaction of the
Abeta(25 - 35 ) fragment with lipid membranes , studied by Electron Spin Resonance spectroscopy , using spin - labeled membrane components ( either phospholipids or
cholesterol ) . -
pubmed
The ESR results show that iAbeta5p influences the
Abeta(25 - 35 ) interaction with the bilayer through a
cholesterol - mediated mechanism : iAbeta5p withholds
cholesterol in the inner hydrophobic core of the bilayer , making the interfacial region more fluid and capable to accommodate
Abeta ( 25 - 35 ) . -
pubmed
Concretely , AuNPs having
poly ( 4 - vinylpyridine ) (
P4VP ) on the surface were first fabricated through surface - initiated atom transfer radical polymerization (
SI - ATRP ) . -
pubmed
The bimetallic nanostructures of AuNP@
P4VP nanocomposites containing another type of metal
NP at the
P4VP periphery exhibit a controlled sensing property in terms of the change in the refractive index of surrounding media and a typical electrocatalytic activity for
methanol oxidation reaction . -
pubmed
Fasting blood
glucose , oral
glucose tolerance , serum lipid profile , tissues glycogen content ,
glucose 6 - phosphatase and
hexokinase enzymes level in liver , in vitro and in vivo
insulin estimation were measured on last day of treatment . -
pubmed
High
glucose predisposes gene expression and
ERK phosphorylation to apoptosis and impaired
glucose - stimulated
insulin secretion via the cytoskeleton . -
pubmed
Chronic HG exposure which reduced
glucose - stimulated
insulin secretion ( GSIS ) increased transcript levels of 185 genes that clustered primarily in 5 processes namely cellular growth and proliferation ; cell
death ; cellular assembly and organization ; cell morphology ; and cell - to - cell signaling and interaction . -
pubmed
These studies revealed a depolymerized
actin cytoskeleton that lacked
actin stress fibers anchored at
vinculin - containing focal adhesion sites as well as loss of
E - cadherin - mediated cell - cell adherence after exposure to chronic HG , and were concomitant with constitutive
ERK1 / 2 phosphorylation that was refractory to serum and
glucose deprivation . -
pubmed
Although inhibition of
ERK phosphorylation by
PD98059 promoted
actin polymerization , it increased apoptosis and GSIS impairment . -
pubmed
Co - over - expression of
MNSFbeta and
Bcl - G reduced
S - nitrosoglutathione - induced
ERK1 / 2 phosphorylation . -
pubmed
Glycoprotein misfolding in the endoplasmic reticulum : identification of released
oligosaccharides reveals a second
ER - associated degradation pathway for Golgi - retrieved proteins . -
pubmed
In the present work , analysis of the released , free
oligosaccharides (
FOS ) derived from all glycoproteins undergoing ERAD , has allowed a global estimation of the mechanisms of this pathway rather than following model proteins through degradative routes . -
pubmed
The
Glc3Man7GlcNAc2 FOS species has been shown to be produced in the
ER lumen by a mechanism involving a peptide : N - glycanase - like activity , and its production was sensitive to disruption of Golgi -
ER trafficking . -
pubmed
The detection of this
oligosaccharide was unaffected by the overexpression of
EDEM1 or cytosolic
mannosidase , both of which increased the production of previously characterised cytosolically localised
FOS . -
pubmed
Ecto-5 '- nucleotidase (
CD73 ) is expressed on intestinal epithelial cells , and it is hypothesized to protect against toxin - induced epithelial damage through the cleavage of
5 '- AMP to
adenosine (
Ado ) and subsequent activation of
adenosine receptors (
AdoRs ) . -
pubmed
Transepithelial electrical resistance ( TEER ) , paracellular
fluorescein isothiocyanate (
FITC)-dextran flux , and
tight junction protein (
ZO-1 ) integrity were monitored . -
pubmed
Concurrent treatment with
5 '- AMP attenuated these responses to
Cdf toxin ; an effect that was blocked with
ZM241385 (
AdoRA2 antagonist ) . -
pubmed
APCP , a
CD73 inhibitor , also suppressed the protective effects of
5 '- AMP on paracellular flux . -
pubmed
5 '- AMP reduced toxin - induced disruption of
ZO-1 , an effect that was abolished by
APCP and
ZM241385 . -
pubmed
Inhibition of
CD73 with
APCP during
Cdf toxin exposure led to increased intestinal barrier permeability and epithelial damage in vivo . -
pubmed
5 '- AMP treatment attenuated the damaging effects of Cdf toxin in vitro , and inhibitors of
CD73 (
APCP ) and
AdoRs (
ZM241385 ) revealed that the cleavage of
5 '- AMP to
Ado was necessary for the protective effects . -
pubmed
We showed previously that three cytoplasmic
Ser /
Thr kinases ,
LKB1 ,
SAD - A , and
SAD - B , control early axon - dendrite polarization in forebrain neurons . -
pubmed
The convulsant effects of
alpha - thujone are attributed to inhibitory actions on the
GABAA receptor . -
pubmed
However , it should not be discarded that acute stress associated with novelty may have induced the recruitment of a subpopulation of
GABAA receptors more sensitive to
alpha - thujone than to the constitutive receptors , or that this
monoterpene could have inhibited any protein or enzyme trafficking that modulated the phosphorylation of the receptor involved in the turnover of
GABAA receptor . -
pubmed
Filgrastim is a
rhG - CSF analogue and is marketed under various brand names , including Neupogen ( ) ( Amgen ) ,
Imumax ( ) ( Abbott Laboratories ) ,
Neukine ( ) ( Intas Biopharmaceuticals ) and others . -
pubmed
The enzyme
5alpha - reductase catalyzes the transformation of
progesterone ,
testosterone , and
deoxycorticosterone into 5alpha - reduced metabolites , which are recognized as
neurosteroids in the brain with variable potential neuroactivity . -
pubmed
Interestingly , immunolocalization studies with antibodies targeting
peroxiredoxin indicate differential localization of the protein in the cell during
metronidazole stress . -
pubmed
In normal conditions , all the Entamoeba isolates exhibit presence of
peroxiredoxin in the nucleus as well as in the membrane ; however with
metronidazole stress the protein localized mostly to the membrane . -
pubmed
Colocalization pattern of
peroxiredoxin with
CaBp1 , a cytosolic protein , revealed that the membrane and nuclear localization was specific to
peroxiredoxin during
metronidazole stress . -
pubmed
Evidence for the participation of
Ca ( 2 +)- activated chloride channels in
formalin - induced acute and chronic nociception . -
pubmed
In this study we determined the role of
Ca ( 2 +)- activated chloride channels (
CaCC ) in acute and chronic nociceptive responses elicited by 1 %
formalin . -
pubmed
Local peripheral and intrathecal pre - treatment ( -10 min ) with the non - selective and selective
CaCC blockers
niflumic acid and
CaCCinh - A01 , respectively , prevented
formalin - induced flinching behavior mainly during phase 2 of the
formalin test . -
pubmed
Moreover , local peripheral and intrathecal
post - treatment ( on day 6 ) with both
CaCC blockers decreased the established
formalin - induced secondary mechanical
allodynia and
hyperalgesia behavior in both paws . -
pubmed
Formalin injection increased
anoctamin-1 , but not
bestrophin-1 protein levels at 6 days . -
pubmed
Intrathecal injection of the
CaCC inhibitor
CaCCinh - A01 prevented
formalin - induced
anoctamin-1 increase . -
pubmed
Data suggest that peripheral and spinal
CaCC , and particularly
anoctamin-1 , participates in the acute nociception induced by
formalin as well as in the development and maintenance of secondary mechanical
allodynia and
hyperalgesia . -
pubmed
Thus ,
CaCC activity contributes to neuronal excitability in the process of nociception induced by
formalin . -
pubmed
Decreases in the levels of
C - reactive protein ,
fibrinogen , and D - dimer were not associated with decreases in the levels of
triacylglycerol and
low - density lipoprotein cholesterol . -
pubmed
The first region accounts for the differences between J-1 and PL-1
gp16 and showed sequence similarity to
carbohydrate - binding modules ( CBMs ) . -
pubmed
Saponarin (
SA ) , a natural
flavonoid , is known for its antioxidant and hepatoprotective activities . -
pubmed
In vivo treatments with the pharmacological
AnxA1 mimetic peptide (
Ac2 - 26 ) or the
formyl peptide receptor (
FPR ) antagonist (
Boc-2 ) were used to elucidate the pathway of
AnxA1 action , and with the cytosolic glucocorticoid antagonist receptor
RU 38486 . -
pubmed
AnxA1 and
FPR2 were constitutively expressed in bone marrow granulocytes , and their expressions were reduced by treatment with
RU38486 . -
pubmed
When present , the severity of
periodontitis and its relationship with gender , smoking , age ,
corticosteroids dose and
AR activity were assessed . -
pubmed
Further study indicated that
berberine inhibited
MRSA amyloid fibrils formation , which consist of
phenol - soluble
modulins ( PSMs ) . -
pubmed
Collectively ,
berberine can inhibit
MRSA biofilm formation via affecting PSMs ' aggregation into amyloid fibrils , and thereby enhance bactericidal activity of antibiotics . -
pubmed
Upon
tamoxifen administration at postnatal day 21 , the floxed
mHtt - exon1 was removed and
mHtt expression was terminated ( Q97(CRE ) ) . -
pubmed
The present study was aimed to investigate the role of
curcumin in
myocardial infarction (
MI ) and its potential mechanism involving
transcription factor specific protein 1 (
SP1 ) . -
pubmed
In
hypoxia - induced
MCM ,
curcumin led to the decrease of cell apoptosis . -
pubmed
Transfected
MCM with miR-7a / b inhibitor ,
curcumin induced the decrease of cell apoptosis and
SP1 expression was reversed . -
pubmed
Transfected with pcDNA -
SP1 , the decrease of cardiac myocytes apoptosis after the treatment of
curcumin was also reversed . -
pubmed
CONCLUSION :
Curcumin pre - treatment protected against
hypoxia - induced cardiac myocytes apoptosis through the up - regulation of miR-7a / b and the down - regulation of
SP1 expression . -
pubmed
To identify proteins essential for the IBB477 specific adhesion property , nine deletion mutants in chromosomal genes have been constructed and analysed using adhesion tests on bare
polystyrene as well as mucin- ,
fibronectin- or
collagen IV - coated
polystyrene plates in comparison to the wild - type
strain . -
pubmed
These experiments demonstrated that gene AJ89_07570 encoding a protein containing
DUF285 ,
MucBP and four Big_3 domains is involved in adhesion to bare and mucin - coated
polystyrene . -
pubmed
To avoid aeration and enhance
oxygen - utilization efficiency , columnar activated
carbon with half of its surface coated by
polytetrafluoroethylene (
PTFE - coated
CAC ) was fabricated as biocathode material , and its performance was investigated using a tide - type biocathode
MES ( TBMES ) . -
pubmed
The TBMES with
PTFE - coated biocathode achieved a maximum power density of 8.2 + - 0.8 W m-3 , which was 39 % higher than that of the untreated control (
CAC biocathode ) . -
pubmed
The
transaminase (
TAm ) catalyzed kinetic resolution of racemic
amino alcohols provides a straightforward approach to access these important compounds . -
pubmed
Moreover , this method can be used to detect
transaminase activity and enantioselectivity toward
amino alcohols in a high - throughput format . -
pubmed
The endocytosis of
N - cadherin , a
calcium - dependent adhesion molecule , has been implicated in the regulation of neurite outgrowth , but the mechanism remains unclear . -
pubmed
Two
tyrosine - based motifs in the cytoplasmic domain of
N - cadherin recognized by the
mu2 subunit of the
AP-2 adaptor complex are responsible for CME of
N - cadherin . -
pubmed
Moreover ,
beta - catenin , a core component of the
N - cadherin adhesion complex , inhibits
N - cadherin endocytosis by masking the 2
tyrosine - based motifs . -
pubmed
RESULTS : The chemical combination of
5 - hydroxymethyl-2 - furaldehyde (
5 - HMF ) ,
parishin B (
PB ) and
parishin C ( PC ) was discovered and confirmed as QCMs of GET by the OPLS - DA method . -
pubmed
No infant in the
MSC group was discharged with home supplemental
oxygen compared with 22 % in the comparison group . -
pubmed
The
NP samples were prepared by the thermal decomposition of an organic
iron precursor subjected to varying reaction conditions , yielding variations in the
NP size , shape and relative crystallinity . -
pubmed
These reduce
low - density lipoprotein cholesterol by over 50 % across a range of populations and were generally found to have a safety profile comparable with placebo . -
pubmed
The ODYSSEY OUTCOMES trial comparing
alirocumab to placebo is expected to report in 2018 and provide cardiovascular outcome data in a
post acute coronary syndrome population . -
pubmed
The phase
II ORION-1 study using
inclisiran , a small interference RNA to
PCSK9 , suggested that two doses of
inclisiran produced time averaged reductions in
LDL cholesterol of 50 % over 9 months . -
pubmed
The analysis of the same polymorphisms showed association at the P value & lt;0.05 with nutrients ( fat ,
SFA , and
polyunsaturated fatty acid - PUFA and
saccharose ) and elevated
LDL level . -
pubmed
The majority of patients in both & gt;=50 and & lt;50 age groups received a non -
nucleoside reverse transcriptase inhibitor - based regimen ( 89 % vs. 87 % ) , did not have
AIDS at baseline ( 63 % vs. 62 % ) , and were male ( 59 % vs. 58 % ) . -
pubmed
AIM : This study aims to explore the accuracy , specificity and laws of axillary lymph node
metastasis predicted by sentinel lymph node biopsy ( SLNB ) by comparing axillary lymph node status via SLNB and axillary lymph node dissection ( ALND ) with
nanocarbon as the tracer . -
pubmed
Hypermethylated
cytosines were evenly distributed throughout the genome with a slight preference for
AP-1 (
JUN and
FOS ) binding sites . -
pubmed
Hypermethylated
cytosines tended to be enriched within the binding sites of transcription factors that showed increased gene expression after
VHL inactivation . -
pubmed
The present study aims to investigate whether
ONO-5046 , a novel
neutrophil elastase inhibitor , could affect NETs formation and promote
inflammation resolution . -
pubmed
ROS was detected by
DCFH - DA fluorescent staining and NETs formation was demonstrated via immunofluorescence staining for
neutrophil elastase and
citrullinated histone H3 ( H3Cit ) . -
pubmed
ONO-5046 reduced LPS - induced NETs formation in a dose - dependent manner , as evidenced by immunofluorescence staining for
neutrophil elastase and
H3Cit whereby the fluorescence intensity decreased and neutrophil ROS generation was attenuated . -
pubmed
CONCLUSIONS : The
neutrophil elastase inhibitor
ONO-5046 suppresses ROS - associated NETs formation , which may lead to
inflammation resolution . -
pubmed
In this study , we constructed a pharmacophore model based on a series of
flavin adenine dinucleotide (
FAD)-competing inhibitors bearing
triazole - dithiocarbamate scaffold combining docking , structure - activity relationship (
SAR ) study , and molecular dynamic ( MD ) simulation . -
pubmed
On the basis of the two pharmacophore models , multi - step virtual screenings ( VSs ) were performed against substrate - binding pocket and
FAD - binding pocket , respectively , combining pharmacophore - based and structure - based strategy to exploit novel
LSD1 inhibitors . -
pubmed
Considering the binding mode and
SAR of the series of compounds , we could roughly deem that these compounds containing
3 - methylxanthine scaffold act through occupying substrate - binding pocket competitively . -
pubmed
Total plasma
triglyceride and
VLDL -
triglyceride concentrations decreased after calorie restriction and exercise ( all p & lt;= 0.025 ) ; the pattern of change was linear with an increasing energy deficit ( all p & lt ; 0.03 ) , with no evidence of plateauing . -
pubmed
Our data indicate that an acute negative energy balance induced by calorie restriction and aerobic exercise reduces
triglyceride concentrations in a dose - dependent manner , by decreasing circulating large and medium
VLDL particles . -
pubmed
Amitriptyline attenuates
bleomycin - induced
pulmonary fibrosis : modulation of the expression of
NF - kappabeta ,
iNOS , and
Nrf2 . -
pubmed
Lung injury was evaluated through the histological examination and immunohistochemical detection of
alpha - smooth muscle actin (
alpha - SMA ) in lung tissue , in addition to the biochemical assessment of pulmonary contents of
hydroxyproline and
transforming growth factor beta-1 (
TGF - beta1 ) . -
pubmed
In addition , the following parameters were investigated for studying the possible mechanisms of
amitriptyline antifibrotic effect :
inducible nitric oxide synthase (
iNOS ) ,
nuclear factor - kappabeta (
NF - kappabeta ) ,
tumor necrosis factor - alpha (
TNF - alpha ) ,
serpine-1 ,
p53 ,
nuclear factor erythroid 2 - related factor 2 (
Nrf2 ) , lipid
peroxides , and
reduced glutathione (
GSH ) . -
pubmed
Amitriptyline successfully suppressed the expression of
NF - kappabeta ,
Nrf2 ,
iNOS , and
p53 in lung tissues besides the inhibition of other oxidative stress and inflammatory mediators . -
pubmed
lusitaniae
strain for the expression of full - length
FKS1 alleles harbouring known mutations contributing to reduced susceptibility or resistance to
caspofungin , thus opening the way for the screening of other
FKS1 /
FKS2 mutations potentially involved in
RES . -
pubmed
Relative to
Delta9 - tetrahydrocannabinol (
THC ) , the synthetic cannabinoid
CP 55,940 (
CP ) is significantly more potent and efficacious at
cannabinoid receptors , the primary targets for endogenous
cannabinoids ( eCBs ) . -
pubmed
A potent and selective irreversible
CDK7 inhibitor
THZ2 was able to induce cell growth inhibition , cell cycle arrest at
G2 / M phase and apoptosis with the increasing intracellular reactive oxidative species ( ROS ) levels in
gastric cancer cells . -
pubmed
Despite surgery and
IV vancomycin with MIC of 1 , suggesting extreme susceptibility , the patient 's blood cultures remained persistently
bacteremic at day 5 of treatment . -
pubmed
Our results demonstrate that overexpression of
phytoglobins allows plants to more efficiently use atmospheric
NO as N source . -
pubmed
Application of
CIP after
p25 insult reverses the pathological changes and
behavioral abnormalities . -
pubmed
ROS -mediated
p53 activation by
juglone enhances apoptosis and autophagy in vivo and in vitro . -
pubmed
Further experiments proved that
H2O2 was a major factor since the
H2O2 scavenger
catalase (
CAT ) reduced both autophagy and cell
death to a greater extent than the
O2 - scavenger
SOD . -
pubmed
The released ions could not only kill microbes by destroying their membrane and inducing the outflow of nutrient substance , but also activate
copper / zinc superoxide dismutase (
Cu / Zn - SOD ) to eliminate
oxygen free radicals and rescue the cells from oxidative stress
injury . -
pubmed
By expressing
channelrhodopsin in hESCs - derived
glutamate neurons and co - culturing them with
GABA neurons , we found that blue light stimulation increased the frequency of miniature excitatory postsynaptic currents ( mEPSCs ) and decreased the ratio of paired
pulse facilitation ( PPF ) in non -
ChR2 - expressing
GABA neurons , indicating a facilitating action at the presynaptic terminals . -
pubmed
Rosmarinic acid reverses
non - small cell lung cancer cisplatin resistance by activating the
MAPK signaling pathway . -
pubmed
Our data demonstrated that
RA significantly inhibited
NSCLC cell proliferation and cell colony formation in a dose - dependent manner , induced
G1 phase cell cycle arrest and apoptosis , and increased the sensitivity of cell lines resistant to
DDP . -
pubmed
Mechanistically ,
RA inhibited
NSCLC cell growth , arrested cell cycle , and induced apoptosis by activating
MAPK and inhibiting the expression of
P - gp and
MDR1 , which correspondingly enhanced
p21 and
p53 expression . -
pubmed
We demonstrate that
RA is a potentially effective MDR reversal agent for
NSCLC , based on downregulation of
MDR1 mRNA expression and
P - gp . -
pubmed
Bovine serum albumin determination based on
methylene blue detection by photothermal lens spectroscopy . -
pubmed
We report on a new sensitive method , for
bovine serum albumin quantification , which is based on the use of
methylene blue as a labelling agent combined with photothermal lens detection . -
pubmed
In the presence of
sodium dodecyl sulfate ,
methylene blue forms a dimer which can react with
bovine serum albumin producing dedimerization . -
pubmed
We found that , the photothermal signal decreases proportional to the concentration of
bovine serum albumin added to the system composed by
dodecyl sulfate and
methylene blue . -
pubmed
CONCLUSIONS : We show that staining for
AFB on
BAL material in lung transplant patients had limited benefit when concurrent microbiology was performed . -
pubmed
Bone morphogenetic proteins (
BMPs ) belonging to the
transforming growth factor - beta ( TGF - beta ) family control the dorsal - ventral ( DV ) patterning of embryos , whereas other morphogens such as
fibroblast growth factor (
FGF ) , Wnt family members , and
retinoic acid ( RA ) regulate the formation of the anterior - posterior ( AP ) axis . -
pubmed
Trace elements , such as
copper (
Cu ) , have been reported to be capable of inhibiting
prolyl hydroxylases leading to the accumulation and activation of
hypoxia - inducible factor-1alpha (
HIF-1alpha ) , a major transcription factor regulating the expression of
VEGF . -
pubmed
pastoris cell growth and may regulate the
alcohol oxidase1 (
AOX1 ) promoter via regulatory factors activated by
methanol - mediated stimulation . -
pubmed
Thirdly , the unfolded protein response ( UPR ) and
ER - associated degradation ( ERAD ) pathways were not significantly upregulated during the
methanol induction period . -
pubmed
Finally , the upregulation of the autophagy pathway during the
methanol induction phase may be related to the degradation of damaged peroxisomes but not to the production of
phytase . -
pubmed
After euthanasia performed one month later , histological analysis was made using
hematoxylin - eosin and anti -
proColXIalpha1 . -
pubmed
Modulation of
EZH2 ,
Snail ,
eIF4E ,
IMPDH ,
mTOR , and
cyclin D1 were observed in western blots and enzymatic activity assays in response to
ribavirin treatment . -
pubmed
Our work suggests that
NPC responds to
ribavirin - mediated
EZH2 ,
Snail ,
eIF4E ,
IMPDH , and
mTOR changes and positions
ribavirin for clinical evaluation as a potential addition to our NPC treatment armamentarium . -
pubmed
We then discuss the knotted states that arise in the intrinsically disordered
polyglutamine and
alpha - synuclein . -
pubmed
What 's more , having experienced 8 weeks of FHJ intervention , KEGG pathways of two - component system ,
ATP - binding cassette ( ABC ) transporters , and biosynthesis of
amino acids made the most differentially expressed genes more abundant , the unigene numbers are 16781,480 , and 1,221 , respectively . -
pubmed
Here , this study reports a kind of conductive film (
PAM - dc - fGO ) fabricated by cross - linking intrinsic self - repair
polyazomethine (
PAM ) and
ethylenediamine - functionalized
graphene oxide ( fGO ) through dynamic covalent bonds (
imine bonds , -
CH N - ) . -
pubmed
Renal oxidative stress was also evaluated by measuring
superoxide dismutase and
malondialdehyde in renal tissues . -
pubmed
DNA was removed from the preparation by two cycles of pancreatic
DNase digestion and
phenol extraction . -
pubmed
These additional sequences were contained in
AAV RNA
molecules present in the pellet fraction , whereas the 20S AAV mRNA accumulated in the supernatant . -
pubmed
Increased oncogenic potential of
ErbB is associated with the loss of a
COOH - terminal domain
serine phosphorylation site . -
pubmed
Here we show that the mutation of
erbB at this negative regulatory
serine phosphorylation site causes fibroblast transformation in vitro and is associated with an increased oncogenic potential in vivo . -
pubmed
This requirement questions the significance of the homology between
La / SS - B and a
retroviral gag polypeptide in the generation of the B cell response to
La / SS - B and is in accord with the general concept that B cells recognize conformational epitopes on
antigens rather than small linear
peptide sequences . -
pubmed
Spare receptors were detected for
oxotremorine - M ,
methacholine and
carbachol , but not the
rest of the agonists , by comparing the receptor - occupancy curves with the concentration - response curves . -
pubmed
A very sensitive and specific method for the simultaneous assay of the activities of two key regulatory enzymes in
cholesterol metabolism ,
3 - hydroxy-3 - methylglutaryl - coenzyme A ( HMG - CoA ) reductase (
EC 1.1.1.34 ) , and
cholesterol 7 alpha - hydroxylase (
EC 1.14.13.7 ) , is described . -
pubmed
Response rates during
DD indicated a dissociation of rate depressant effects and discriminative performance following
ethanol . -
pubmed
The technique of competitive chromogenic substrate hydrolysis is used to examine the inhibitory effects of
sucrose and
glycerol on the inactivation of
thrombin by
antithrombin III . -
pubmed
The evidence suggests that a component from each of the mitochondrial
NADH - coenzyme Q ,
succinate - coenzyme Q , and
coenzyme QH2 - cytochrome c reductases ( complexes I ,
II , and III ) , most likely a nonheme
iron -
sulfur protein of each complex , is involved in free radical formation . -
pubmed
Continuous
VP infusion reduced significantly 24 - h amounts of WI and UO , and increased
Na+ excretion . -
pubmed
Substance P and
5 - HT do not seem to share a common release mechanism . -
pubmed
Changes in antibody production were not related to plasma levels of
cortisol ,
corticosteroid - binding globulin activity , or
testosterone . -
pubmed
Ca2 + -antagonistic properties of
phospholipase A2 inhibitors ,
mepacrine and
chloroquine . -
pubmed
Both agents decreased slow
calcium channel current Isi and fast
sodium channel current If . -
pubmed
Role of plasma
adenosine in the antiplatelet action of
HL 725 , a potent inhibitor of
cAMP phosphodiesterase : species differences . -
pubmed
The inhibitory action of
HL 725 can be reversed by washing and resuspension of the platelets , suggesting that
HL 725 does not bind tightly to
cAMP PDE . -
pubmed
Similar blockade of the inhibitory actions of several other inhibitors of
cAMP PDE such as
RA 233 ,
RX - RA 69 ( analogs of
dipyridamole ) and
oxagrelate is seen by
adenosine deaminase pretreatment . -
pubmed
However , if the whole blood is pretreated with
adenosine deaminase , no inhibitory effect of
dipyridamole plus
HL 725 is seen on
platelet aggregation . -
pubmed
Intrastriatal
kainic acid injections resulted in a total disappearance of the stimulatory effects of
Glu on evoked - release of
[ 3H ] DA as well as in a parallel reduction in the maximal number ( Bmax ) of a
D - aspartate - insensitive
L -[ 3H ] Glu binding site in striatal particulate fractions . -
pubmed
There was no significant level of
3H - thymidine uptake by cultures of
SLE or normal mononuclear cells in response to either
SLE or normal
ACS . -
pubmed
[ Effects of
dithiocarbamate : " in vitro " and " in vivo " changes in
gamma - glutamyltransferase induced by
sodium diethyldithiocarbamate ] . -
pubmed
Experiments of
NaDDTC effect on kidney and 10,000 X g liver supernatant fraction have shown no modification of normal
GGT activity , also after dialysis . -
pubmed
It is suggested that liver
MFO produces
SH groups derived from
NaDDTC metabolism able to interact with
GGT and consequential inactivation of the
enzyme . -
pubmed
beta - Adrenergic blockade with
propranolol displaced the
glucose production ( MANOVA , P = 0.0220 ) and plasma
glucose ( MANOVA , P = 0.0057 )
somatostatin response curves to higher levels , whereas alpha - adrenergic blockade with
phentolamine combined with beta - adrenergic blockade displaced the
glucose production ( MANOVA , P = 0.0281 ) and plasma
glucose ( MANOVA , P = 0.0134 )
somatostatin response curves to lower levels . -
pubmed
Nucleoids released from a
Ncd clone give two peaks in a
sucrose gradient with a characteristic S value for each nucleoid . -
pubmed
Mechanism of synthesis of
adenosine(5')tetraphospho(5')
adenosine (
AppppA ) by
aminoacyl - tRNA synthetases . -
pubmed
An early and transient postoperative rise in
calcitonin (
CT ) corresponding to a decline in
calcium levels was demonstrated . -
pubmed
The polysaccharide -
iron - regulated cell surface protein conjugate (
PS - IRCSP ) was non - toxic and non - pyrogenic . -
pubmed
Recombinant
alpha 1 - antitrypsin with a
P1 arginine residue (
Arg - alpha 1 - antitrypsin ) is a rapid inhibitor of both
thrombin and
activated protein C (
APC ) . -
pubmed
For example , replacement of the
P2 proline of Arg -
alpha 1 - antitrypsin by
glycine decreased the association rate constant ( kass ) with
thrombin by 37 - fold while the kass value with
APC was reduced by only 16 - fold . -
pubmed
The decrease in the kass for
thrombin caused by the mutation of the
P2 proline to
alanine or
glycine was 3 - fold greater when
threonine was present in the P'1 position instead of the normal
serine . -
pubmed
Substitution of
proline for the
P2 glycine of this chimeric
serpin increased the kass values with
thrombin and
APC by 7- and 90 - fold , respectively . -
pubmed
The effect of the
P2 substitution was again found to depend on the sequence surrounding the residue ; the change in the kass for
APC caused by the
P2 Pro-->
Gly replacement was 6 - fold larger in the chimeric
serpin . -
pubmed
Evaluation of the kass values of the chimeric
serpin with a
P2 proline in light of the likely rates of inhibition of
thrombin and
APC during antithrombotic therapy with
heparin suggested that this
serpin may have kinetic parameters suitable for an antithrombotic agent . -
pubmed
In individual fibers , the activities of
malate dehydrogenase (
MDH ,
citric acid cycle ) ,
lactate dehydrogenase (
LDH , glycolysis ) and
adenylokinase ( high - energy
phosphate ) were quantified . -
pubmed
Evidence that the inhibitory effects of
iodide on thyroid cell proliferation are due to arrest of the cell cycle at G0G1 and
G2 M phases . -
pubmed
The addition of
methimazole at two concentrations ( 0.2 and 2 mM ) to cells exposed to 100 mM
NaI prevented the accumulation of cells in
G2 M , but did not abolish the accumulation of cells in G0G1 or the reduction in cell number . -
pubmed
It appears that organified
iodine may mediate the cell cycle arrest in the
G2 M phase , whereas inorganic
iodide may be responsible for the inhibitory effects at G0G1 . -
pubmed
Treatment of membranes with
PrBCM results in a selective inactivation of
M3 muscarinic receptors , while treatment with
EEDQ results in a relative sparing of
M1 muscarinic receptors . -
pubmed
We have previously shown that
dopamine , working through
D1 - like dopamine receptors , alters the
PLC response to
carbachol by preventing this shift in the apparent EC50 for
GTP gamma
S16 . -
pubmed
When we reproduced these experiments in
EEDQ- and
PrBCM - treated membranes , only in
EEDQ - treated membranes was
dopamine able to inhibit the
PLC response to
carbachol . -
pubmed
The eventual targets of
retinoids are
retinoic acid receptors (
RARs ) . -
pubmed
Therefore , developmental expression patterns of
RARs are important in elucidating the mechanisms of normal functions of
retinoids and
retinoid - induced
teratogenesis . -
pubmed
Overlapping expression and redundancy of various
RARs may be one of the safeguard mechanisms in
retinoid homeostasis . -
pubmed
Point mutations of
Thr-10 to
Arg and
Tyr-13 to
Ile greatly lowered
MCP-1 activity . -
pubmed
Insertion of a
Pro between these two residues , or their substitution by the sequence
Gly - Pro - His , caused nearly complete loss of
MCP-1 activity . -
pubmed
This sexual dimorphism is apparently also due , in part , to a suppressive effect of circulating
androgens on [
leptin ] . -
pubmed
Phospholipids inhibit proteolysis of
protein kinase C alpha by mM
calcium - requiring
calpain . -
pubmed
The alpha isoform of
protein kinase C (
PKC alpha ) is rapidly hydrolyzed by mM
Ca ( 2 +)-requiring calpain (
calcium - activated neutral proteinase ) under cell - free conditions ( Shea et al , 1994 , FEBS Lett . -
pubmed
With the exception of
phosphatidic acid , these
phospholipids did not directly inhibit
calpain activity as evidenced by degradation of
[ 14C ] azocasein , suggesting that the nature of inhibition of calpain - mediated
PKC alpha degradation is due to an effect of
phospholipids on
PKC alpha conformation . -
pubmed
In addition , the unique inhibition of calpain activity by
phosphatidic acid suggests the existence of a specific mechanism by which this
phospholipid regulates
PKC alpha activity . -
pubmed
The inhibitory effects on sexual behavior and ambulatory activity of the mixed
GABAA / GABAB agonist
progabide are differentially blocked by
GABA receptor antagonists . -
pubmed
It was concluded that the
GABAA but not the
GABAB receptor is important for the inhibitory effects of
progabide on that behavior . -
pubmed
This suggests that the motor effects of
progabide are mediated by either a non - GABAergic receptor or by a subtype of the
GABAA or the
GABAB receptor that is not sensitive to the antagonists . -
pubmed
Their abnormal
hemoglobin was about 52 % of the total
hemoglobin , and was shown to be stable by the
isopropanol test . -
pubmed
Whole blood
P50 of the proband was 19.3 Torr , Bohr effect was decreased ( -0.25 ) , as well as the molar ratio between the
2,3 - diphosphoglycerate level and total
hemoglobin of erythrocytes ( 0.68 ) . -
pubmed
The purified abnormal
hemoglobin was characterized by an altered
oxygen affinity , low n - factor ,
chloride , and
2,3 - diphosphoglycerate effects . -
pubmed
The
myelin - associated / oligodendrocyte basic proteins (
MOBPs ) are recently discovered constituents of myelin and are small , cytoplasmic , and highly
basic proteins exclusively expressed postnatally by oligodendrocytes . -
pubmed
Due to a clustering of positively charged
amino acids observed in the most abundant
MOBP isoform similar to
myelin basic protein (
MBP ) and
P0 , it was speculated that
MOBP could function in myelin sheath compaction . -
pubmed
This
ketol isomerase converts the
aldose L - fucose into the corresponding
ketose L - fuculose using
Mn2 + as a cofactor . -
pubmed
The
protein environment suggests strongly that the reaction belongs to the
ene - diol type . -
pubmed
Pretreatment with
Nomega - nitro - L - arginine methyl ester blocked
IGF - I - induced increases in
PTA in single myocytes . -
pubmed
Consistent with its inotropic actions on muscles and myocytes ,
IGF - I induced a dose - dependent increase in
Ca2 + transients in normal but not
diabetic myocytes . -
pubmed
Mechanisms underlying this alteration in
IGF - I - induced myocardial response may be related to changes in intracellular
Ca2 + and/or
NO production in
diabetes . -
pubmed
Thyroid hormone receptor beta - dependent expression of a
potassium conductance in inner hair cells at the onset of hearing . -
pubmed
dHAND and
eHAND are two related
basic helix - loop - helix transcription factors that are expressed in a complementary fashion in the developing right and left ventricles , respectively . -
pubmed
Efficient export of the
glucose transporter
Hxt1p from the endoplasmic reticulum requires
Gsf2p . -
pubmed
We have isolated the
HXT1 gene , which encodes a low - affinity , high - capacity
glucose transporter , as a multicopy suppressor of a
gsf2 mutation . -
pubmed
gsf2 mutants also display a
galactose growth defect and abnormal localization of the
galactose transporter
Gal2p but are not defective in function or localization of the high - affinity
glucose transporter
Hxt2p . -
pubmed
These findings suggest that
Gsf2p functions in the
ER to promote the secretion of certain
hexose transporters . -
pubmed
Kinetic , structural and electrostatic aspects of the reduction of
pentacyanoferrate ( III ) complexes by
myoglobin . -
pubmed
The results of this and an earlier study show that an outer - sphere mechanism is operating for electron transfer between
oxymyoglobin and
FeIII ( CN ) 5Ln - , independent of the lability of the ligand L. The electron transfer process is preceded by precursor formation at a specific site on the protein close to the
protein heme pocket . -
pubmed
Biopharmaceutical profile of
cerivastatin : a novel
HMG - CoA reductase inhibitor . -
pubmed
Following administration ,
cerivastatin was rapidly and almost completely absorbed into the gastrointestinal tract ( & gt ; 98 % ) , with maximum plasma concentrations ( Cmax ) reached at 2 - 3 h
post dose . -
pubmed
Vagotomy and administration of
atropine abolished the gastric acid secretion induced by
ghrelin . -
pubmed
Hindquarters were perfused with 7 mmol / l
glucose , 1,000 micromol / l
albumin - bound
palmitate , and
albumin - bound
[ 1 -( 14 ) C ] palmitate at
rest ( no
insulin ) . -
pubmed
Modulation of
Na , K - ATPase and
Na - ATPase activity by phospholipids and
cholesterol . -
pubmed
The effects of phospholipid
acyl chain length ( n(c ) ) , degree of
acyl chain saturation , and
cholesterol on
Na , K - ATPase reconstituted into liposomes of defined lipid composition are described . -
pubmed
In addition , the increased bilayer order induced by both
cholesterol and saturated phospholipids could be important for the conformational mobility of the
Na , K - ATPase changing the distribution of conformations . -
pubmed
The temperature dependence of the
Na , K - ATPase as well of the
Na - ATPase reactions depends both on phospholipid
acyl chain length and on
cholesterol . -
pubmed
Cholesterol increased significantly both the enthalpy of activation and entropy of activation for
Na , K - ATPase activity and
Na - ATPase activity of
Na , K - ATPase reconstituted with monounsaturated phospholipids . -
pubmed
In the case of
ATPase reconstituted without
cholesterol , the minimum free energy of activation and the maximum turnover both shifted to longer
acyl chain lengths of about 22 . -
pubmed
Repression of
GAD autoantigen expression in pancreas beta - Cells by delivery of antisense plasmid /
PEG - g - PLL complex . -
pubmed
To repress
GAD expression in islet beta - cells , we delivered an antisense
GAD mRNA expression plasmid ( pRIP -
AS -
GAD ) using
poly ( ethylene glycol )-grafted
poly - L - lysine (
PEG - g - PLL ) as a gene carrier . -
pubmed
In a gel retardation assay , the
pRIP - AS -
GAD /
PEG - g -
PLL complex was completely retarded above a weight ratio of 1:1.5 ( plasmid :
PEG - g -
PLL ) . -
pubmed
PEG - g -
PLL protected the plasmid DNA from
DNase I for more than 60 minutes . -
pubmed
Therefore , our study revealed that the pRIP - AS -
GAD /
PEG - g - PLL system is applicable for the repression of
GAD autoantigen expression . -
pubmed
The levels of total serum
bilirubin ( P<0.05 ) and
serum transaminases ( P<0.05 ) were significantly higher in patients with
AIH -
AH than in patients with AIH - CH and
AIH - LC . -
pubmed
Frizzled - related protein (
Frp ) is a new family of secreted proteins that contain a region homologous to the extracellular
cysteine - rich domain (
CRD ) of the frizzled family proteins . -
pubmed
The 876 - bp
bla(CTX - M-17 ) gene differed from
bla(CTX - M-14 ) by 2 nucleotides , which led to the single
amino acid substitution Glu289 - ->
Lys . -
pubmed
[ Protective effect of low concentration
endothelin-1 on the reactive
oxygen - induced inhibition of pulmonary surfactant lipid synthesis ] . -
pubmed
The effects of
endothelin-1 (
ET-1 ) at low concentration ( 1 - 100 pmol / L ) on the reactive
oxygen - induced inhibition of both pulmonary surfactant ( PS ) lipid synthesis and the activity of
CTP : phosphorylcholine cytidylyltransferase (
CCT ) , a rate - limiting enzyme in biosynthesis of
phosphoatidylcholine ( PC ) , were studied in cultured lung explants without serum . -
pubmed
The
xanthine - xanthine oxidase superoxide anion generating system decreased
( 3 ) H - choline incorporation into PC in a dose - dependent manner in cultured lung explants . -
pubmed
ET-1 reduced both the reactive
oxygen - induced decrease in
( 3 ) H - choline incorporation and the increase in
malondialdehyde (
MDA ) content of lung tissues , but did not change the levels of antioxidant enzymes
superoxide dismutase (
SOD ) ,
catalase (
CAT ) and the total antioxidant capability in the lung explants . -
pubmed
ET-1 also prevented the inhibitive effect of reactive
oxygen on microsomal
CCT activity in the lung explants . -
pubmed
The cDNA contained an open reading frame encoding 71
amino acid residues and the sequences for
CdlP1 and
CdlP2 were found to be located in the center of this coding region . -
pubmed
The
CMP - sialic acid synthetase (
CMP - Neu5Ac , synthetase ) is responsible for the synthesis of
CMP - Neu5Ac , which is the donor used by
sialyltransferases to attach
sialic acid to acceptor
hydroxyl groups in various polysaccharides ,
glycolipids , and glycoproteins . -
pubmed
Since
CMP - Neu5Ac is unstable and relatively expensive , the
CMP - Neu5Ac synthetase is valuable for the preparative enzymatic synthesis of
sialylated oligosaccharides . -
pubmed
The purified
CMP - Neu5Ac synthetase was used in the gram - scale synthesis of
CMP - Neu5Ac . -
pubmed
Impact of
obesity and
leptin levels on the secretion of
estradiol ,
inhibin A and
inhibin B during ovarian stimulation with
gonadotropins . -
pubmed
Serum levels of
estradiol ,
leptin ,
inhibin A and
inhibin B were measured . -
pubmed
The results suggest that high
leptin concentrations in vivo are not associated with impaired secretion of
estradiol and dimeric inhibins during ovarian stimulation . -
pubmed
Here we report that
ura4 + , encoding
orotidine monophosphate decarboxylase (
OMPdecase ) , shows
glucose - inducible expression regulated at
post - transcriptional level . -
pubmed
The
ura4 + mRNA level was rapidly decreased by approximately 50 % within 20 min after
glucose depletion and it was readily recovered upon
glucose - readdition within 1 h.
Glucose at above 2 % similarly raised the transcript level of
ura4 + , while low concentration ( 0.1 % ) was not effective . -
pubmed
Interestingly , control of mRNA turnover would be the main regulatory step of the
glucose - dependent expression of
ura4 + . -
pubmed
These results suggest that the
SAPK pathway might participate in the
glucose - dependent regulation of
ura4 + and
rrg1 + mRNA stabilities . -
pubmed
As concerns follow - up of the
CR patients , one did not receive any further treatment and died in
CR from
pulmonary aspergillosis , and one with a concomitant
chronic lymphocytic leukemia (
CLL ) received two courses of FLAG (
fludarabine ,
cytarabine ,
granulocyte colony - stimulating factor ) regimen with disappearance of the clonal
Ig rearrangement , but relapsed after 11 months and died from
pneumonia . -
pubmed
Analysis of uterine
oestrogen receptor alpha revealed that
naringenin significantly increased the cytosolic concentration of
oestrogen receptor alpha , whereas in nuclei the
oestrogen receptor alpha concentration was significantly decreased as compared to the solvent control . -
pubmed
This was in contrast to the positive control
17 beta - oestradiolacetate which acted as a true
oestrogen by increasing the concentration of both total and nuclear
oestrogen receptor alpha . -
pubmed
Both
naringenin and
17 beta - oestradiolacetate , however , significantly , induced nuclear
oestrogen receptor alpha in the liver , suggesting a tissue specific effect of
naringenin on
oestrogen receptor alpha distribution . -
pubmed
Similarly , exposure to
glutamate or
staurosporine induced significantly more neuronal cell
death in
galanin knockout organotypic and dispersed primary hippocampal cultures than in WT controls . -
pubmed
Further , exogenous
galanin or the previously described high - affinity
GALR2 agonist , both reduced cell
death when coadministered with
glutamate or
staurosporine in WT cultures . -
pubmed
The
HER-2 - targeting antibodies
trastuzumab and
pertuzumab synergistically inhibit the survival of
breast cancer cells . -
pubmed
Trastuzumab (
herceptin ) and
pertuzumab (
Omnitarg , 2C4 ) are recombinant humanized monoclonal antibodies that target different extracellular regions of the
HER-2 tyrosine kinase receptor . -
pubmed
Infrared ( IR ) spectroscopy using a smart endurance single bounce diamond attenuated total reflection (
ATR ) cell has been used to study the changes in the spectra of the surfactant
octadecyltrimethylammonium (
ODTMA )
bromide upon intercalation into a
sodium montmorillonite . -
pubmed
Using a phosphospecific antibody to
Syk (
Tyr525 /
Tyr526 ) that measures activated
Syk , we found that most ( 60%-75 % ) of the active
Syk is in the ubiquitinated fraction . -
pubmed
The role of N - glycosylation in the stability , trafficking and
GABA - uptake of
GABA - transporter 1 . -
pubmed
Terminal N - glycans facilitate efficient
GABA - uptake activity of the
GABA transporter . -
pubmed
The
GABA transporter
GAT1 belongs to the family of
Na(+)- and
Cl(-)-coupled transport proteins which possess 12 putative transmembrane domains and three N - glycosylation sites in the extracellular loop between transmembrane domain 3 and 4 . -
pubmed
This indicates that
N - glycans , in particular their terminal trimming , are important for the
GABA - uptake activity of
GAT1 . -
pubmed
For example , the
ATP - binding cassette (
ABC ) drug transporters have been shown to protect
cancer stem cells from chemotherapeutic agents . -
pubmed
The structures of
N - ethyl-3 -( 4 - fluorophenyl )- 5 -( 4 - methoxyphenyl )- 2 - pyrazoline-1 - thiocarboxamide ,
C19H20FN3OS , ( I ) , and
3 -( 4 - fluorophenyl )- N - methyl-5 -( 4 - methylphenyl )- 2 - pyrazoline-1 - thiocarboxamide ,
C18H18FN3S , (
II ) , have similar geometric parameters . -
pubmed
The
methoxy / methyl - substituted phenyl groups are almost perpendicular to the
pyrazoline (
pyraz ) ring [ interplanar angles of 89.29 ( 8) and 80.39 ( 10 ) degrees for ( I ) and (
II ) , respectively ] , which is coplanar with the
fluorophenyl ring [ interplanar angles of 5.72 ( 9 ) and 10.48 ( 10 ) degrees ] . -
pubmed
We investigated the bioavailability of DSM Nutritional Products Ltd. ( Kaiseraugst , Switzerland )
CoQ10 10 %
TG / P ( all - Q ) , a new tablet - grade formulation , with CoQ10 Q - Gel Softsules based on the Bio - Solv technology ( Tishcon Corp. , Salisbury , MD ; marketed by Epic4Health , Smithtown , NY ) and Q - SorB ( Nature 's Bounty , Bohemia , NY ) . -
pubmed
The disease - modifying treatments ,
IFN - beta and
glatiramer acetate , have been widely available over the last decade and have shown a beneficial effect on relapse rate and magnetic resonance imaging parameters of disease activity ; however , their effect on
disease progression and disability is modest . -
pubmed
These include humanized monoclonal antibodies such as
daclizumab (
IL-2 antagonist ) , oral immunomodulators such as
sirolimus and statins and neuroprotective agents such as NMDA antagonists and
Na +- channel blockers . -
pubmed
Overall
Tween-20 extracts effectively decreased levels of
TNF - alpha ,
IL-1 ,
IL-2 and
IL-6 as observed using
cytokine bioassays . -
pubmed
Tween-20 extracts effectively inhibited both
COX-1 and
COX-2 cyclooxygenase activity . -
pubmed
Although the anti -
cytokine activity of the
Tween-20 extract was destroyed by
Proteinase - K treatment , the anti -
COX-1 and anti -
COX-2 activity of both the extracts were not sensitive to
protease treatment . -
pubmed
Surgical specimens were sliced transversely on serial embedded blocks at 2.5 - mm intervals , and stained with
hematoxylin and eosin (
HE ) . -
pubmed
Tumor - suspect nodules previously considered disease free by
HE stain on whole - mount section were examined by in situ hybridization ( ISH ) on tissue microarray ( TMA ) through detecting mRNAs of
CEA and
CK20 with non radioactive
biotin - tagged oligonucleotide probes . -
pubmed
Alpha - galactosidase (
alpha - Gal ) enzyme , which is encoded by the
melA gene hydrolyzes
alpha-1,6 galactoside linkages found in
sugars , such as
raffinose and
stachyose . -
pubmed
MEASUREMENTS AND RESULTS : Four hours after LPS injection , the antioxidant enzyme activities , including
superoxide dismutase (
SOD ) ,
glutathione peroxidase (
GPx ) , and
thiobarbituric acid reactive substance ( TBARS ) levels as an indicator of lipid peroxidation , were determined . -
pubmed
Intestinal apoptosis was assessed by
hematoxylin -
eosin staining and
terminal deoxynucleotide transferase - mediated
fluorescein -
dUTP nick end labeling . -
pubmed
Damage to ICC and pacemaking was greatly attenuated in the absence of
NO derived from
iNOS . -
pubmed
Downregulation of
c - FLIP promotes
caspase - dependent
JNK activation and reactive
oxygen species accumulation in
tumor cells . -
pubmed
Nuclear factor - kappa B (
NF - kappaB ) inhibits cell
death through suppression of the
caspase cascade , the
c - Jun N - terminal kinase (
JNK ) pathway , and reactive
oxygen species ( ROS ) accumulation . -
pubmed
New studies support this idea by showing that the catalysis of
vitamin A into
retinoic acid ( RA ) in gut - associated dendritic cells ( DCs ) enhances the
transforming growth factor ( TGF )- beta - dependent conversion of naive T cells into regulatory T ( T reg ) cells and also directs T reg cell homing to the gut . -
pubmed
Binding of
dioxygen to a non -
heme enzyme has been modeled using the ONIOM combined quantum mechanical / molecular mechanical ( QM / MM ) method . -
pubmed
For the present system ,
isopenicillin N synthase (
IPNS ) , binding of
dioxygen is stabilized by 8 - 10 kcal / mol for a QM : MM ( B3LYP : Amber )
protein model compared to a quantum mechanical model of the active site only . -
pubmed
In the
protein system , the free energy change of
O2 binding is close to zero . -
pubmed
The second major factor that stabilizes
O2 binding is van der Waals interactions between
dioxygen and the surrounding
enzyme . -
pubmed
Both
GF and
CL expression are highly induced at the onset of fruit ripening , coincident with the ripening - associated decline in
chlorophyll . -
pubmed
The
SGR subfamily is required for
chlorophyll degradation and operates through an unknown mechanism . -
pubmed
Levels of fasting total - C ,
HDL - C ,
LDL - C ,
triglycerides ,
homocysteine , and
lipoprotein ( a ) were measured . -
pubmed
After 6 months of the therapy , serum levels of total - C ,
LDL - C , and
homocysteine decreased significantly ( P=.04 , P=.02 , P=.008 , respectively ) in the treated group when compared with the control group . -
pubmed
Moreover , deletion of N - terminus , proximal
acidic domain or , surprisingly , zinc finger domains leads to
Rpn4p stabilization . -
pubmed
We found that the non - selective
NOS inhibitor
7 - nitroindazole (
7 - NI ) as well as the more selective neuronal and inducible
NOS inhibitor
1 -( 2 - trifluoromethylphenyl ) imidazole (
TRIM ) decreased the number of marbles buried . -
pubmed
Inhibition of
nNOS seems to play a key role in the behavioral effects of
NOS inhibitors , whereas
agmatine seems to have a different mechanism of action . -
pubmed
Nevertheless , exposure to the environmental xenobiotic
2,3,7,8 - tetrachlorodibenzo - p - dioxin (
TCDD ) , a well - known exogenous ligand of the
Ahr pathway , led to overexpression of the
Ahr target gene
Cyp1a1 , and to increased sensitivity to
TCDD - triggered apoptosis in
E2F2(-/- ) T cells compared with WT controls . -
pubmed
An artificial model of photosynthetic
photosystem II : visible - light - derived
O2 production from water by a
di - mu - oxo - bridged manganese dimer as an
oxygen evolving center . -
pubmed
Visible - light - derived
O ( 2 ) production was yielded by conjugating water oxidation catalysis by
[ ( OH ( 2 ))( terpy ) Mn ( mu - O )( 2 ) Mn ( terpy )( OH ( 2 ))]( 3 + ) as an
oxygen evolving center model and photo - sensitization of
[ Ru ( bpy )( 3 )]( 2 + ) as a photoexcitation center model at an interlayer of
mica . -
pubmed
On 23 October 2009 , the commissioner of the US Food and Drug Administration ( FDA ) issued an Emergency Use Authorization ( EUA ) for
peramivir for intravenous injection , an unapproved
neuraminidase inhibitor used for treating certain hospitalized adult and pediatric patients infected with 2009
H1N1 influenza . -
pubmed
METHODOLOGY / PRINCIPAL FINDINGS : We tested the possibility that
L - DOPA might interfere with
DAT binding . -
pubmed
CONCLUSIONS / SIGNIFICANCE : These data indicate that
L - DOPA does not induce modifications of
DAT expression detectable by SPECT of by DAT binding autoradiography , suggesting that differences between clinical assessment and radiotracer imaging in clinical trials may not be specifically related to
L - DOPA treatment . -
pubmed
Expression of drug - transporting
P - glycoprotein (
P - gp , an
integral protein of the plasma membrane ) in neoplastic cells confers multidrug resistance and also involves alteration of cell sensitivity to inhibitors of the
sarco / endoplasmic reticulum calcium pump thapsigargin ( Th ) . -
pubmed
Purification and characterization of
cystathionine beta - synthase bearing a
cobalt protoporphyrin . -
pubmed
Metal content analysis showed that the
enzyme contained 92 %
cobalt and 8 %
iron . -
pubmed
CoCBS was indistinguishable from wild - type
FeCBS in its activity , tetrameric oligomerization ,
PLP saturation and responsiveness to the allosteric activator ,
S - adenosyl - l - methionine . -
pubmed
The coordinators of these processes may be small GTPases of the
Rho family and their activators , the factors of exchange of
guanylic nucleotides . -
pubmed
Decreased
RRP22 expression levels were in part explained by 5'-CpG island hypermethylation and increased by the treatment with the demethylating agent
5 - aza-2 '- deoxycytidine in
glioblastoma cell lines . -
pubmed
In addition , the in vitro treatment with the
histone deacetylase inhibitor
trichostatin A alone resulted in
RRP22 reexpression as well as a significant increase in the levels of
RRP22 promoter DNA bound to pan - acetylated histone
H3 and
H4 . -
pubmed
In a laboratory study , formulations of 15 % ( w / w ) of ordinary Portland cement ( OPC ) ,
calcium aluminate cement (
CAC ) and pozzolanic cement ( PC ) and additives : plasticizers cementol delta ekstra ( PCDE ) and
cementol antikorodin (
PCA ) ,
polypropylene fibers ( PPF ) ,
polyoxyethylene -
sorbitan monooleate (
Tween 80 ) and aqueous
acrylic polymer dispersion ( Akrimal ) were used for solidification / stabilization ( S / S ) of soils from an industrial brownfield contaminated with up to 157 , 32,175 , 44,074 , 7614 , 253 and 7085 mg kg(-1 ) of
Cd ,
Pb ,
Zn ,
Cu ,
Ni and
As , respectively . -
pubmed
The THG experiments for type I and type
II LCB crystals were performed . -
pubmed
Thin films of
nickel and
lanthanum -
strontium - manganite ( LSM ) are grown on
silicon substrate at 573 K using selected metallorganic complexes of
Ni [( acac ) 2en ] ,
La ( tmhd ) 3 ,
Sr ( tmhd ) 2 and
Mn ( tmhd ) 3 as precursors by plasma assisted liquid injection chemical vapor deposition (
PA - LICVD ) . -
pubmed
Serum
alanine aminotransferase ,
aspartate aminotransferase ,
gamma - glutamyl ,
alkaline phosphatase ,
bilirubin , and
albumin levels , presentation patterns , and radiologic findings were assessed . -
pubmed
The basal
[ ( 35 ) S ] GTPgammaS binding was changed by varying the
GDP concentration whilst the basal
F - actin contents of the higher expressing T cell populations were elevated , suggesting constitutive activity of
CCR4 . -
pubmed
RESULTS The affinity of
CCL17 ( also known as
TARC ) derived from analysis of the T cell data ( pK(a ) = 9.61 + - 0.17 ) was consistent with radioligand binding experiments ( 9.50 + - 0.11 ) while that from the
[ ( 35 ) S ] GTPgammaS binding experiments was lower ( 8.27 + - 0.09 ) . -
pubmed
For this reason an LC -
MS -
MS method was developed for detection of wide range of NSAIDs , including both " acidic " NSAIDs (
carprofen ,
diclofenac ,
flunixin ,
meloxicam ,
phenylbutazone ,
oxyphenbutazone ,
tolfenamic acid ,
mefenamic acid ,
naproxen ,
ketoprofen ,
ibuprofen ,
firocoxib ,
rofecoxib , and
celecoxib ) and " basic " NSAIDs ( four
metamizole metabolites ) . -
pubmed
All NSAIDs were separated on a Phenomenex Luna C8(2 ) column and analyzed by LC -
MS -
MS in negative ( acidic NSAIDs ) and positive (
metamizole metabolites ) ion modes . -
pubmed
OsbZIP16 was shown to be induced by exogenous
ABA treatment , while overexpression of
OsbZIP16 was observed to make transgenic plants more sensitive to
ABA than wild type plants were . -
pubmed
Intracerebroventricular injections of
6 - OHDA induced a bilateral loss of
tyrosine hydroxylase - positive cells in the substantia nigra ( -51 % ) and ventral tegmental area ( -31 % ) . -
pubmed
Sequence analysis has shown that
Spy0843 contains two
leucine - rich repeat ( LRR ) domains that mediate interactions with the
gp340 receptor . -
pubmed
Elucidation of the crystal structure will provide insights into the interactions of
Spy0843 with the
gp340 receptor and a better understanding of the role of
Spy0843 in
streptococcal infections . -
pubmed
The abundance of
cysteines in this isoform is particularly fascinating , as
hVDAC-1 cysteines have no associated functional role . -
pubmed
We report a detailed biophysical examination of a
Cys - less mutant of
hVDAC-2 , and its behavioral comparison with the wild type protein . -
pubmed
These findings suggest that
HyaB and
HydA can function as uptake
hydrogenases that couple the oxidation of
H2 to the reduction of amaranth to sustain cellular growth . -
pubmed
Assays with respiratory inhibitors indicated that a
menaquinone pool and different
cytochromes were involved in the azoreduction process . -
pubmed
The fact that certain
MHC - I receptors have the unusual capacity to recognize
MHC - I molecules expressed by other cells ( trans ) and by the NK cell itself ( cis ) has raised the question regarding possible contributions of the two types of interactions to NK cell education . -
pubmed
OBJECTIVE : Evaluate
ustekinumab , an anti -
interleukin ( IL )- 12 and
IL-23 antibody , effects on radiographic progression in
psoriatic arthritis (
PsA ) . -
pubmed
Treatment included an
insulin infusion ,
omega-3 fatty acid esters , and
gemfibrozil . -
pubmed
The
insulin infusion reduced serum
triglyceride levels by 50 % after 1 day of treatment and to 355 mg / dL by day 7 of her ICU course . -
pubmed
Specimens were randomly assigned to five groups according to the disinfectant used : Group I : Control ( no disinfectant ) ; Group
II : 5.25 %
sodium hypochlorite based ; Group III : 2 %
chlorhexidine based ( Consepsis ) , Group
IV : 0.1 %
benzalkoniumchloride based (
Tubulicid red ) and Group V : 3 %
doxycycline based ( Biopure , MTAD ) . -
pubmed
RESULTS : Dentin disinfectants tested significantly negated the bonding of Adper Single bond 2 and the groups were ranked ; Group I & gt ; Group V = Group
IV & gt ; Group
II = Group III , meanwhile they enhanced significantly the mu - SBS values upon using
Clearfil S ( 3 ) Bond and were ranked ; Group
II & gt ; Group III = Group
IV = Group V & gt ; Group I. -
pubmed
Efficacy of some non - conventional herbal medications (
sulforaphane ,
tanshinone IIA , and
tetramethylpyrazine ) in inducing neuroprotection in comparison with
interleukin-10 after
spinal cord injury : A meta - analysis . -
pubmed
OBJECTIVES : To assess the efficacy of some non - conventional herbal medications including
sulforaphane ,
tanshinone IIA , and
tetramethylpyrazine in reducing
inflammation and compare them with a known effective anti - inflammatory agent (
interleukin-10 (
IL-10 ) ) . -
pubmed
Tetramethylpyrazine has an acceptable influence in reducing
inflammation through the up - regulation of
IL-10 . -
pubmed
Outcomes of
sulforaphane and
tanshinone IIA administration are acceptable but still weaker than
IL-10 . -
pubmed
AIM : Hip arthrography usually requires the injection of
iodine based dyes which can cause complications . -
pubmed
In addition , anti -
cancer activities were confirmed by conducting
MTT assay for
ART and
ART - CLN on different
cancer cell lines . -
pubmed
Glucagon - like peptide-1 (
GLP-1 ) is a metabolic hormone that is secreted in a
glucose - dependent manner and enhances
insulin secretion . -
pubmed
The results suggest that exendin-4 potentiates a subpopulation of extrasynaptic
GABAA receptors in the
CA3 pyramidal neurons . -
pubmed
Phosphorylation of the transcription factor
cAMP - responsive element binding protein (
CREB ) significantly increased in cells treated with high
glucose ( HG ) compared to cell grown in normal
glucose ( NG ) . -
pubmed
Cells pretreated with
rapamycin before exposure to HG showed significant decrease phosphorylation of
CREB , increase in
AMPK activity and decrease protein / mRNA and promoter activity of
fibronectin . -
pubmed
Cells treated with HG showed nuclear localization of
p - CREB while pretreated cells with
rapamycin reversed HG effect . -
pubmed
Moreover , gel shift analysis shows increase binding of
CREB to
fibronectin promoter in cells treated with HG while cells pretreated with
rapamycin reversed the effect of HG . -
pubmed
Excessive accumulation of
histamine in the body leads to miscellaneous symptoms mediated by its bond to corresponding receptors (
H1 - H4 ) . -
pubmed
HOXA13 decreased
beta - catenin ,
phospho -
smad2 , and
phospho -
smad3 in the nucleus and increased
phospho -
beta - catenin in the cytoplasm . -
pubmed
RASD1 has been shown to inhibit
adenylyl cyclase activity in vitro resulting in the inhibition of the
cAMP -
PKA -
CREB signaling pathway . -
pubmed
Therefore , we tested the hypothesis that
RASD1 may inhibit
cAMP stimulated gene expression in the brain . -
pubmed
RESULTS : We show that
Rasd1 is expressed in
vasopressin neurons of the PVN and
SON , within which mRNA levels are induced by hyperosmotic cues . -
pubmed
These effects were dependent upon isoprenylation , as both
farnesyltransferase inhibitor
FTI-277 and CAAX box deletion prevented
Rasd1 inhibition of
cAMP - induced gene expression . -
pubmed
We propose that the abundance of
RASD1 in
vasopressin expressing neurons , based on its inhibitory actions on
CREB phosphorylation , is an important mechanism for controlling the transcriptional responses to stressors in both the PVN and
SON . -
pubmed
These effects likely occur through modulation of
cAMP -
PKA -
CREB signaling pathway in the brain . -
pubmed
The
enzyme was completely inhibited by
PMSF and significantly inhibited by
EDTA ,
TPCK ,
Co ( 2 + ) ,
Zn ( 2 + ) , and
Cu ( 2 + ) , suggesting a
chymotrypsin - like serine metalloprotease . -
pubmed
On
post injury day ( PID ) 1 , 2 , 3 , the vital signs , volume of fluid input , and volume of the urine output were observed and recorded ; femoral artery blood was drawn to determine
lactate ,
bicarbonate radical , and base excess , and
oxygen index was calculated . -
pubmed
At
PIH 12 , 24 , 48 , 72 , femoral vein blood was drawn to determine white cell count , platelet count , neutrophils ,
creatine kinase - MB ,
creatine kinase ,
lactic dehydrogenase ,
aspartate transaminase (
AST ) ,
alanine aminotransferase (
ALT ) ,
creatinine ,
urea nitrogen , and blood
glucose ( the ratio of
AST to
ALT was calculated ) . -
pubmed
Superelastic (
SE ) and thermo - activated ( TA )
nickel -
titanium (
NiTi ) archwires are used in everyday orthodontic practice , based on their acceptable biocompatibility and well - defined shape memory properties . -
pubmed
The
cytotoxic effect of Rematitan
SE correlated with the higher release of
Ni ions in CM , higher concentration of surface
Ni and an increased
oxygen layer thickness after the conditioning . -
pubmed
In this protocol , the quantitative derivation of the
spring - constant and drag - coefficient of the bond between adhering colloidal particles and substratum surfaces using QCM - D is explained for bacteria and
silica particles , using the particle - mass derived for validation . -
pubmed
Dopamine D2 receptors mediate the increase in reinstatement of the conditioned rewarding effects of
cocaine induced by acute social defeat . -
pubmed
In the present study we evaluated the effect of the
dopamine D1 - and D2 - like receptor antagonists (
SCH23390 and
raclopride , respectively ) on the short - time effects of acute social defeat (
ASD ) . -
pubmed
A final experiment was designed to evaluate the effect of the
dopamine antagonists on the
CPP induced by 3mg / kg of
cocaine with or without a stress experience . -
pubmed
Blockade of
dopamine D2 - like receptors with
raclopride specifically prevented the effects of stress without affecting the rewarding properties of
cocaine . -
pubmed
Our results confirm that the
dopamine D2 receptor is involved in the short - term effects of
ASD on the rewarding effects of
cocaine . -
pubmed
The
dopamine D1 receptor is clearly involved in the rewarding effects of
cocaine , but its role in the effects of
ASD remains to be demonstrated . -
pubmed
Hierarchical regression was used to evaluate if gratitude , social support , and satisfaction with life moderated the relationship between stressful life events ( as measured by the total life stress score ) and
PTG , after adjustment for age , sex , race , level of involvement in Hurricane Katrina , and
alcohol intake . -
pubmed
Imatinib treatment not only prevented
ankle swelling and
synovial inflammation when administered before
MSU crystals but also diminished these features when administrated after the injection of
MSU crystals , a therapeutic protocol more closely mimicking the clinical situation in which treatment occurs after the development of an acute
gout flare . -
pubmed
A simple , mild and reproducible synthetic route was developed to fabricate the multifunctional
Au@
carbon /
calcium phosphate core - shell nanoparticles (
Au@C / CaP NPs ) , which act as an efficient nanoplatform for pH / near - infrared ( NIR ) dual - responsive drug delivery , X - ray computed tomography (
CT ) imaging and synergistic chemo - photothermal
cancer therapy in vitro . -
pubmed
Deciphering protein dynamics changes along the pathway of
retinol uptake by cellular
retinol - binding proteins 1 and 2 . -
pubmed
CRBP 1 and 2 , sharing a 56 %
amino acid sequence identity , exhibit the highest binding affinities for
retinol . -
pubmed
Herein , the results of molecular dynamics simulations for the uptake of
retinol by
CRBP 1 and 2 are consistent with the presence of two different
retinol entry points , both involving the ' cap region ' ( alpha - helices I and
II and neighboring loops ) . -
pubmed
We observed that a hydrophobic patch at the surface of the ' portal region ' ( alpha - helix
II ,
CD and EF loops ) of
CRBP 1 attracts
retinol , which accesses the binding cavity through an opening generated by the concerted movements of
Arg58 and
Phe57 , present in the CD loop . -
pubmed
In
CRBP 2 a different distribution of the surface residues of the ' cap region ' allows
retinol to access the binding cavity by sinking in a hydrophobic matrix between the two alpha - helices . -
pubmed
Polar interactions mainly affect
retinol movements inside the beta - barrel cavities of both
CRBPs . -
pubmed
More valuable information could probably be provided about the variety of the diseases caused by
superoxide anions
toxicities by intervention and application of the non - viral method for expressions of
SOD1 and
SOD3 enzymes . -
pubmed
The identified
20 - amino acid sequence of the purified peak by Edman degradation shared 100 % identity with the N - terminal regions of
mytichitin-1 ,
mytichitin-2 ,
mytichitin-3 ,
mytichitin-4 ,
mytichitin-5 , and
chitinase - like protein-1 , and so was named
mytichitin - CBD . -
pubmed
An
opioid modulator is a drug that can produce both agonistic and antagonistic effects by binding to different
opioid receptors and therefore can not be classified as one or the other alone . -
pubmed
In summary , the structural elucidation of
opioid receptors , allosteric modulation of
opioid receptors , new
opioid modulators and agonists , the employment of optogenetics , optopharmacology , and next - generation sequencing of
opioid receptor genes and related functionality should create exciting new avenues for research and therapeutic development to treat conditions including
pain ,
opioid abuse , and
addiction . -
pubmed
Further targeted metabolomics by
GC -
MS uncovered the identity of the
deoxyhexose with
1,5 - anhydroglucitol displaying the most significant changes . -
pubmed
Fatty acids (
FA ) are crucial in this process , since they have a dual role as energetic coins and building blocks . -
pubmed
In vitro , we verified that
FA and
estradiol stimulated
FATP1 /
SLC27A1 expression in
BCCs . -
pubmed
Additionally , experiments with
estradiol and
PHTPP (
ER - beta antagonist ) showed that
estrogen receptor - beta (
ER - beta ) regulates
FATP1 /
SLC27A1 expression , the uptake of
FA and cell viability , in four
BCC lines . -
pubmed
Furthermore , the inhibition of
FATP1 with
arylpiperazine 5k (
DS22420314 ) interfered with the uptake of
FA and cell viability . -
pubmed
BACKGROUND :
Fingolimod (
FTY ) applied as treatment regimen of
relapsing - remitting multiple sclerosis (
RRMS ) induces downregulation of
sphingosine-1 - phosphate receptors on the lymphocytes . -
pubmed
Hereby , we report a 71 - year - old female with
seronegative rheumatoid arthritis who presented with massive
splenomegaly ,
pancytopenia , and positivization of antibodies against double - stranded deoxyribonucleic acid ( dsDNA ) after initiation of the
anti - tumor necrosis factor (
TNF )
golimumab . -
pubmed
Withdrawal of the anti -
TNF and treatment with the anti -
CD20 antibody
rituximab were able to revert the clinical abnormalities . -
pubmed
In this paper , we propose a surface plasmon resonance (
SPR ) sensor based on two - dimensional ( 2D ) materials (
graphene ,
MoS2 ,
WS2 and
WSe2 ) hybrid structure , and theoretically investigate its sensitivity improvement in the visible region . -
pubmed
An
Ag - based
SPR sensor exhibits a higher sensitivity than an
Au- or
Cu - based one at most wavelengths in the visible region . -
pubmed
In addition , the sensitivity of the proposed
SPR sensor varies obviously with the wavelength of incident light , and shows a maximum value of 159 , 194 or 155 /RIU for
Au ,
Ag or
Cu , respectively . -
pubmed
Not surprisingly , direct visualization of RGCs by
SLO fundus imaging correlated best with histological quantification of RGC
somata and axons . -
pubmed
Using gas chromatography - mass spectrometry (
GC -
MS ) , we identified
methyl palmitate and
methyl stearate as bioactive compounds . -
pubmed
The effects of OMSW different pretreatments ( microwave or autoclave ) , different additives ( water , formic , or
sulfuric acid ) , and utilization of different saccharification enzymes ( Cellic CTec2 or
Accellerase 1500 ) were tested on saccharification process and
sugar release , and on fermentation inhibitors formation and
ethanol production . -
pubmed
Simplified LC -
MS Method for Analysis of
Sterols in Biological Samples . -
pubmed
The method was validated on 10
sterol standards that were detected in a single chromatographic LC -
MS run without derivatization . -
pubmed
The developed method enables a simple , sensitive , and fast quantification of
sterols , without the need of extended knowledge of the LC -
MS technique , and represents a new analytical tool in the rising field of cholesterolomics . -
pubmed
Multivariate analysis showed that baseline
steroids was an independent predictor for a worse OS ( P<0.001 ) , while performance status ( PS)>=2 was an independent predictor for a poorer
TTF ( P<0.001 ) and OS ( P<0.001 ) . -
pubmed
How
Oxygen - binding Enhances Long - range Electron Transfer : Lessons from Reduction of
Lytic Polysaccharide Monooxygenases by
Cellobiose Dehydrogenase . -
pubmed
Using multiscale simulations , we reveal that interprotein
ET between
CDH and LPMO is mediated by the
heme propionates of
CDH and solvent waters . -
pubmed
Interestingly , we find that
oxygen binding to the
copper center of
LPMO is coupled with the long - range interprotein ET . -
pubmed
The uncovered ET mechanism rationalizes experimental observations and has far - reaching implications in
LPMO catalysis , as well as the O 2 or
CO binding - enhanced long - range ET processes in other
metalloenzymes . -
pubmed
However , inhibiting KATP by
5 - HD and
Gly significantly reversed the effects , including
NLRP3 inflammasome and inflammatory mediators
IL-6 ,
MPO ,
TNF - alpha , and
MCP-1 . -
pubmed
Stimulation of
glucose oxidation by
insulin is reduced in large cells . -
pubmed
Studies using a mimicker of
insulin action ,
spermine , as well as measurements of 125I -
insulin binding to large and small cells indicate that receptor number and affinity are not responsible for hormone resistance . -
pubmed
Furthermore , when rapid and direct measurements of
sugar uptake were made ,
insulin stimulation was virtually identical in both cell types . -
pubmed
These findings indicate that large adipocytes have an efficient
insulin - responsive D -
glucose transport system and suggest that the apparent hormone resistance may be due to alterations in intracellular
glucose metabolism . -
pubmed
Microsomal
mixed - function oxidases and
epoxide hydratase convert
benzo ( a ) pyrene stereospecifically to optically active
dihydroxydihydrobenzo ( a ) pyrenes . -
pubmed
After induction by
TCB ,
EROD was detectable in microsomes from whole thymus , bursa and in peritoneal exudate cells ( containing primarily macrophages ) at levels of 28.3 , 7.2 and 1.3 pmol / mg microsomal
protein / min , respectively ; the level in control liver was 89.9 pmol / mg microsomal
protein / min . -
pubmed
The
P450I specific in vitro inhibitor ,
alpha - naphthoflavone (
NF ) inhibited the
TCB - induced liver and immune tissue
EROD by 50 % at concentrations in the range of 0.07 - 0.1 microM. -
pubmed
Much higher
TCB - induced
EROD was detected in immune tissue supporting cells than in lymphocytes , particularly in the thymus . -
pubmed
TCB - induced
EROD was 12 - fold higher in the lymphocyte - depleted thymus than in normal thymus , with a less marked but similar pattern in the bursa. -
pubmed
Previously it was shown [ 1 ] that
amino acid substitutions at the region of the first alpha - helix of
IL-2 specifically inactivate its reactivity with the
intermediate - affinity receptor p70 , and mutations in the fifth alpha - helix specifically inactivate the binding to the low - affinity receptor
p55 . -
pubmed
We have synthesized the peptides corresponding to the putative binding site of
IL-2 with the
intermediate - affinity receptor p70 and found that the
nonapeptide corresponding to the sequence 27 - 35 of the mature
IL-2 [ 2 ] effectively competes with
human rIL-2 for binding to thymocyte receptors . -
pubmed
Characterization of the
aluminum and
beryllium fluoride species which activate
transducin . -
pubmed
Aluminofluoride and
beryllofluoride complexes can activate the heterotrimeric
G - proteins by binding next to
GDP in the
nucleotide site of their
G alpha subunit and acting as analogs of the
gamma - phosphate of a
GTP . -
pubmed
We have studied , by monitoring the activation - dependent
tryptophan fluorescence of
transducin T alpha subunit , the pF ( -log[F- ] ) and pH dependencies of the kinetics of activation and deactivation of T alpha
GDP in the presence of
NaF and
aluminum or
beryllium salts . -
pubmed
After 2 weeks , the fish were exposed to severe environmental
hypoxia ( final water
oxygen partial pressure , PWO2 = 25 - 45 Torr , reached within 20 min ) , to assess the impact of the experimentally induced
alkalosis , and hence increased
haemoglobin -
oxygen ( Hb -
O2 ) affinity , on various aspects of the
hypoxic response . -
pubmed
This was accomplished by monitoring
oxygen partial pressure and total
oxygen content of arterial blood ( PaO2 and
CaO2 ) , extracellular pH ( pHe ) , red blood cell ( RBC ) intracellular pH ( pHi ) , and the concentrations of plasma
adrenaline ,
noradrenaline ,
lactate and
haemoglobin (
Hb ) at 5 min intervals . -
pubmed
Determination of the PaO2 thresholds for
lactate and
catecholamine release demonstrated that these substances appeared in the bloodstream when the degree of
O2 saturation of the
haemoglobin fell below 60 % . -
pubmed
Cytochrome c , an extrinsic protein , at a 10:1 mole ratio , does not modify the phase - dependent behaviour of the terminal
methyl groups , but consistently shifts all the observed bands to lower - frequency positions , which suggests a long - range effect of the protein along the phospholipid
fatty acyl chains . -
pubmed
Nonpeptidic
angiotensin II antagonists : synthesis and in vitro activity of a series of novel
naphthalene and
tetrahydronaphthalene derivatives . -
pubmed
Starting from the structure of the novel nonpeptidic
angiotensin II antagonist
DuP 753 , a series of more rigid analogues was prepared by replacing the
biphenyl part of
DuP 753 with a
naphthalene ring . -
pubmed
These results may indicate that the compounds do not bind to the
AT1 receptor in the same way as
DuP 753 . -
pubmed
We conclude that ( 1 )
rIFN - alpha does not have a significant
leukaemia - specific effect on the progenitor cells detected in these assays , and ( 2 ) the sensitivity of
CML GM -
CFC to
rIFN - alpha is independent of the type of
BCR /
ABL message present in the cells . -
pubmed
Endoglycosidase F incubated with
C5 followed by
monosaccharide analyses by anion exchange chromatography with pulsed amperometric detection revealed both high
mannose and complex ( terminate in
SA )
oligosaccharides were hydrolyzed ; no effect was found on the functional activity of
C5 . -
pubmed
Approximately 4 % of the complex
oligosaccharides were hydrolyzed from
C5 . -
pubmed
Of the
sialidase - treated
125I - C5 15 % was bound to EAC1gp(4b , oxy2a,3b)hu compared to 9.3 % of buffer - treated
125I - C5 . -
pubmed
Furthermore , 27 % of
sialidase - treated
125I - C5 was bound to EAC1gp,4bhu compared to 16.6 % of buffer - treated
125I - C5 , but no lysis occurred after the addition of
C6 - C9 . -
pubmed
A new approach to determine the stereospecificity in
lipase catalysed hydrolysis using circular dichroism (
CD ):
lipases produce optically active
diglycerides from achiral
triglycerides . -
pubmed
We describe a sensitive CD method for determining the stereospecificity in
lipase ( E.C.3.1.1.3 ) catalysed hydrolysis of
triacyl glycerols into
diacyl glycerols . -
pubmed
The
diglycerols were converted to chiral
tert - butyldimethylsilylated 1,2 - or
2,3 - di - O - benzoyl - sn - glycerol ( 5 or 5 ' ) , and their
CD was measured . -
pubmed
This approach showed for the first time that
lipases produce optically active
diacyl glycerides from achiral
tripalmitin and
tribenzoyl glyceride with a variable extent of enantioselectivity depending on the
acyl groups and the enzymes . -
pubmed
[
Post - treatment evaluation of fillings with
Cermet cement ( Ketac - Silver ) ] . -
pubmed
Rates of total
glucose utilization ,
glucose oxidation , nonoxidative
glucose disposal ( NOGD ) ,
glucose metabolic clearance rate ( MCR ) , and endogenous
glucose production ( EGP ) were determined with a " two - level "
insulin -
clamp technique ( 100 - min infusions at 40 and 400 mU X m-2 X min-1 ) combined with indirect calorimetry and
D-3 -[ 3H ] glucose infusion . -
pubmed
The role of
thiols in lymphocyte responses : effect of
2 - mercaptoethanol on
interleukin 2 production . -
pubmed
Since
2 - ME could conceivably affect
IL2 production and utilization equally , resulting in no apparent change in the
IL2 concentration of treated cultures , 12 - h cultures were used to minimize any effects of
IL2 utilization on the
IL2 concentrations observed . -
pubmed
No effect was observed on
IL2 concentrations at 12h when cultures were treated with 2 - ME under conditions in which the
2 - ME enhanced cellular activation / proliferation . -
pubmed
On the basis of plasma
cotinine determination all real smokers had higher
7 - ethoxyresorufin 0 - deethylase (
ERDE ) activities measured either in placental microsomes or liver biopsy homogenates than non - smokers . -
pubmed
Classification based on plasma
cotinine levels showed a statistically significant ( P less than 0.001 ) difference between smokers and non - smokers in liver homogenate
ERDE activity . -
pubmed
The above studies showed that 3 drugs :
ribamydil ( 50 micrograms / ml ) ,
phosphonoformate ( 120 micrograms / ml ) and
recombinant interferon ( 1000 IU / ml ) were capable of statistically significant inhibition of retrovirus replication in continuous cultures of T - lymphocytes . -
pubmed
However , the optimal dose levels were lower and the effective dose interval more restricted as compared to the previously described
poly [ Lys -( Leui - DL - Alam ) ] (
LAK ) . -
pubmed
Like
LAK , both HAK and
D - LAK were able to compensate for the immunosuppressive effect of the cytotoxic drugs
dianhydrogalactitol ,
vincristine , and
5 - fluorouracil , which all have different mechanisms of action , provided that combined treatment by polypeptide and drug was applied repeatedly before the
SRBC immunization . -
pubmed
Clinical grade
heparin is a very heterogeneous mucopolysaccharide , containing molecules with Mr ranging from 6,000 to 30,000 that have either a high affinity or a low affinity for
antithrombin III (
AT ) . -
pubmed
Equivalent amounts (
carbohydrate moiety ) of covalent complexes of heparin or of both heparin fragments with
human AT resulted in an antithrombotic effect lasting for 45 - 60 min . -
pubmed
Both
[ 3H ] proline and
[ 3 ] fucose were transported transneuronally to the ipsilateral abducens nucleus (
AN ) ; with long survivals [ 3H]proline was transported peripherally via the ipsilateral abducens nerve root . -
pubmed
Photooxidation of
lanthanide ion -
lysozyme complexes . -
pubmed
Basal
adenylate cyclase activity in the testicular membrane fraction increased 20 to 30 % above control in response to
gossypol administration . -
pubmed
Testis membranes from the 20- and 30 - mg treatment group exhibited a 2- and 4 - fold greater activation of
adenylate cyclase by
guanine nucleotides . -
pubmed
In vitro dose - response curves showed a half - maximal inhibitory concentration ( IC50 ) for inhibition of soluble testicular
adenylate cyclase by
gossypol of 400 microM in each treatment group . -
pubmed
During synaptosomal reduction , the electron paramagnetic resonance (
EPR ) high field line of
DMPO - OH is broadened indicating restrictions on the motion of the
nitroxide . -
pubmed
This was not due to simple absorption of
DMPO - OH to
proteins . -
pubmed
Under these conditions , the average
glucose infusion rate ( M ) is an index of
glucose metabolic rate , the average
insulin response ( I ) is an index of beta cell responsiveness to
hyperglycemia , and the M / I ratio is an index of
insulin sensitivity . -
pubmed
The biochemical profile [ levels of
calcium ,
phosphorus ,
magnesium ,
chlorides and
iron , the activities of
alkaline phosphatase (
ALP ) ,
aspartate aminotransferase (
AST ) and
alanine aminotransferase (
ALT ) , and the concentrations of
total protein ,
albumin ,
cholesterol ,
urea ,
glucose , and
vitamins A and E ] was studied in the blood serum of 40 anoestrous and 40 control inseminated animals in a production herd with an increased occurrence of anoestrus in gilts . -
pubmed
The anoestrous gilts showed significantly lower levels of
albumin ( P less than 0.01 ) and
glucose ( P less than 0.01 ) and
ALP activity ( P less than 0.05 ) , and significantly higher concentrations of
urea ( P less than 0.01 ) ,
vitamin A ( P less than 0.01 ) and
vitamin E ( P less than 0.05 ) and
ALT activity ( P less than 0.05 ) , as compared with the inseminated controls . -
pubmed
The comparison of the obtained values of the studied biochemical criteria with literary data indicated a lower concentration of
magnesium and a higher
ALP and
ALT activities in the anoestrous and inseminated gilts in both groups under study . -
pubmed
The inhibitory action of two
thrombin inhibitors (
TI-189 and
TI-233 ) on the contractile responses to
5 - hydroxytryptamine and
prostaglandin endoperoxide analogue (
U-44069 ) in isolated vascular strips . -
pubmed
Microsomes were incubated with
ferrous ion (
Fe2 + ) to promote free radical production , and
alpha - T levels and
LP were monitored after various incubation times . -
pubmed
Coinciding with
alpha - T depletion was a large and rapid increase in
LP . -
pubmed
With outer zone microsomes ,
alpha - T depletion required more than 30 min , and very little
LP was demonstrable during this period . -
pubmed
However , once
alpha - T depletion occurred ,
LP was rapidly initiated and reached levels similar to those obtained with inner zone preparations . -
pubmed
Inhibition of
LP by
MnCl2 prevented the
Fe ( 2 +)-induced declines in alpha - T in both zones . -
pubmed
The results demonstrate the importance of
alpha - T as a modulator of adrenal
LP and indicate that the zonal differences in
LP are largely attributable to the differences in
alpha - T concentrations . -
pubmed
CTLA-4 molecules may either facilitate or down - regulate the second signal to T - cells , which is provided by the interaction between the two accessory
molecules CD28 and
B7 . -
pubmed
An unequivocal answer is given to the question of why the reported fluorescence spectra of
bacteriorhodopsin (
bR568 ) have been different from one another . -
pubmed
Calcium - dependent transcriptional activation through the SRE is mediated by the
serum response factor (
SRF ) . -
pubmed
Membrane depolarization induces phosphorylation of
SRF at
Ser-103 , an event shown to enhance the ability of
SRF to bind the SRE . -
pubmed
Ca ( 2 +)-induced
SRF phosphorylation occurs via a pathway that may involve
Ca2 +/ calmodulin - dependent kinases . -
pubmed
The
sodium ,
potassium ions - activated
adenosine triphosphatase (
Na + , K (+)- ATPase ) activity of the erythrocyte membrane was decreased to 22 % ( P & lt ; 0.01 ) and 24 % ( P & lt ; 0.01 ) in the
NaCl and NaCit groups , respectively , compared with the control groups ; this activity was decreased to 43 % in the ChoCl group ( P & lt ; 0.05 ) . -
pubmed
The improved results may be due to earlier renal biopsy for precise histopathologic definition , better supportive care , and selective use of aggressive therapy , including
methylprednisolone pulse therapy , intravenous
cyclophosphamide , intravenous
prostaglandin E1 therapy , high - dose intravenous
gammaglobulin therapy , and
cyclosporin A for those with high risk factors . -
pubmed
The structure of a catalytically competent reaction intermediate of
carboxypeptidase A ( CPA ) formed with the specific spin - label
ester substrate
O -[ 3 -( 2,2 ,- 5,5 - tetramethyl-1 - oxypyrrolinyl ) propen-2 - oyl ]- L - b eta - phenyllactate through application of cryoenzymological methods has been determined by electron nuclear double resonance ( ENDOR ) and molecular modeling . -
pubmed
It is sensitive to the
dihydropyridines nicardipine and
Bay K 8644 , to
calciseptine , and to an anti - peptide antibody raised against the alpha1 subunit of the
L - type calcium channel , but is voltage - insensitive . -
pubmed
Camptothecin and minor - groove binder hybrid molecules : synthesis , inhibition of
topoisomerase I , and anticancer
cytotoxicity in vitro . -
pubmed
The synthesis , characterization , inhibitory activity against
topoisomerase I , and biological evaluation of a series of 14
camptothecin derivatives of
polypyrrolecarboxamide (
lexitropsin ) conjugates of two structural classes : ( A )
camptothecin - NHCO - lexitropsin 44 - 51 and ( B )
camptothecin - CONH - lexitropsin 38 - 43 are described . -
pubmed
All 16 compounds tested , 14 conjugates plus two functionalized
camptothecin controls , inhibit
topoisomerase I in the concentration range 1.12 - 16.6 microM that divide into three distinct categories based on activity . -
pubmed
The most active
enzyme inhibitors belong to structure class A with either cationic
dimethylaminium or neutral
amide end groups . -
pubmed
Subjects were studied at
rest and during constant cycle exercise at a work rate equivalent to 40 % of their maximum
oxygen consumption . -
pubmed
The aim of this study was to determine the effects of
desflurane on cerebral blood flow (
CBF ) velocity using transcranial Doppler ultrasonography in a setting that closely resembled usual clinical practice . -
pubmed
In group 2 no significant changes in systemic haemodynamic responses were measured after
desflurane administration ; however ,
CBF velocity increased to 73 + /- 5 cm s-1 ( 59 % increase ) . -
pubmed
The results indicate that
desflurane increases
CBF velocity concurrently with induction of
tachycardia and
hypertension . -
pubmed
Although
sufentanil and
N2O attenuate the systemic haemodynamic alterations caused by
desflurane , the
CBF velocity increases . -
pubmed
The inhibitory efficacy of
sulbactam ,
clavulanic acid and
tazobactam over
TEM-1 is inversely proportional to the amounts of enzyme produced . -
pubmed
The three principal
sarcoplasmic / endoplasmic reticulum Ca ++ pump inhibitors have been compared for their effects on
Ca ++ fluxes across intracellular stores present in isolated skeletal muscle and brain membrane preparations . -
pubmed
These observations taken together suggest that transient releases were manifest primarily by vesicles with a high ratio of
ryanodine receptors to pumps ( and thus more susceptible to becoming leaky with only some pumps inhibited ) , and that
Ca(++)-induced
Ca ++ release amplified releases when all pumps were blocked . -
pubmed
One subset of compounds consists of
fadrozole analogues and was studied in a previous work , from which a ' local ' 3 - D quantitative structure - activity relationship ( QSAR ) model for the inhibition of
aromatase was obtained . -
pubmed
In the present paper , that model is extended to include a second subset of compounds bearing a
tetralone nucleus and acting at the same
enzyme site with the same mechanism as the
azoles . -
pubmed
In the present investigation the effects of
NPY on whole cell
Ba2 + currents were examined to obtain direct evidence supporting the mechanisms suggested by those studies . -
pubmed
This inhibition was
pertussis toxin sensitive and resulted from
NPY modulation of L- and N - type
Ca2 + channels . -
pubmed
The inhibition of L - type channels was not seen with & lt ; 1 nM free intracellular
Ca2 + or when
protein kinase C (
PKC ) was inhibited by
chelerythrine or
PKC -( 19 - 31 ) . -
pubmed
Furthermore , the effect of
NPY on L - type channels was mimicked by the
PKC activator
phorbol 12 - myristate 13 - acetate . -
pubmed
Syndecan-1 , a transmembrane
heparan sulphate proteoglycan (
HSPG ) , functions as a matrix receptor on the basal surface of epithelial cells . -
pubmed
Similarly , there was a significant reduction of both
molecules in
carcinomas compared with associated
adenomas (
syndecan-1 P = 0.00003 ;
E - cadherin P = 0.002 ) . -
pubmed
We have previously shown that a range of
nicotinamide containing ' biomimetic coenzymes ' function as active analogues of
NAD + in the oxidation of
alcohols by
horse liver alcohol dehydrogenase (
HLADH ) , despite their apparently astonishing lack of structural similarity to the natural coenzyme . -
pubmed
An HPLC - based assay suggested that two of the new analogues were coenzymically active in the oxidation of
butan-1 - ol by
HLADH , with one displaying a significantly higher activity than
CL4 . -
pubmed
Ventilator management was aimed at maintaining comparable values of end tidal
carbon dioxide ,
Hb saturation , and arterial blood gases . -
pubmed
Multitargeted antifolate (
MTA ,
LY231514 ) is a novel antifolate antimetabolite , with antitumor activity via inhibition of
thymidylate synthase ,
glycinamide formyl transferase , and
dihydrofolate reductase . -
pubmed
Intrapleural therapy with
MDP - Lys (
L18 ) , a synthetic derivative of
muramyl dipeptide , against
malignant pleurisy associated with
lung cancer . -
pubmed
N2 -[( N - acetylmuramoyl )- L - alanyl - D - isoglutaminyl ]- N6 - stearoyl - L - lysine (
MDP - Lys (
L18 ) ,
romurtide ) is a synthetic
muramyl dipeptide derivative , and has immunomodulating activities including activation of cells of monocyte - macrophage lineage . -
pubmed
We examined the effect of intrapleural instillation of
MDP - Lys (
L18 ) against
malignant pleurisy associated with
lung cancer . -
pubmed
These results suggest
MDP - Lys (
L18 ) instilled by intrapleural route had a potential local immunomodulatory activity . -
pubmed
Chiral monodentate
phosphine ligand
MOP for
transition - metal - catalyzed asymmetric reactions . -
pubmed
Luminal epithelial cells , glandular epithelial cells and stromal cells were isolated from cyclic , pregnant or
oestrogen - induced pseudopregnant gilts on Day 12 ( Experiment 1 ) or Day 16 ( Experiment 2 )
post oestrus ( oestrus = Day 0 ) . -
pubmed
Polynitroxyl
albumin ( PNA ) is a compound of
human serum albumin covalently labeled with
nitroxides that exhibits a prolonged half - life and an enhanced antioxidant activity . -
pubmed
Discordant results were distributed equally among specimens from drug - experienced and drug - naive individuals and were slightly higher for
protease inhibitors than for
nonnucleoside reverse transcriptase inhibitors or
nucleoside reverse transcriptase inhibitors . -
pubmed
Glucose released from storage in liver glycogen by
phosphorylase accounts for approximately 50 % of the
glucose produced after an overnight fast . -
pubmed
However , the same combination reduced
HLGPa activity , and the inhibition was
glucose dependent . -
pubmed
The positive staining for
GLUT-2 observed for islets cells and all
islet tumors is consistent with the notion that all pancreatic islet cells and
islet cell tumors utilize
glucose as a major fuel , requiring transporter - facilitated diffusion of
glucose into the cells of normal organ and their
tumors . -
pubmed
Structurally diverse
carbon - linked ( C - linked ) analogs of
antifreeze glycoprotein (
AFGP ) have been prepared via linear or convergent solid phase synthesis . -
pubmed
Sulfa resistance and
dihydropteroate synthase mutants in recurrent
Pneumocystis carinii pneumonia . -
pubmed
The results suggest that
DHPS mutations can be selected de novo within patients by the pressure of a
sulfa or
sulfone drug . -
pubmed
It was recently discovered that
VGLUT1 , previously characterized as an inorganic
phosphate transporter (
BNPI ) with 9 - 11 predicted transmembrane spanning domains , is capable of transporting
glutamate . -
pubmed
The enzyme
20alpha - hydroxysteroid dehydrogenase (
20alpha - HSD ) catalyzes the conversion of
progesterone into its inactive form ,
20alpha - hydroxyprogesterone . -
pubmed
These results suggest that
progesterone can be inactivated by
20alpha - HSD in some specific brain areas . -
pubmed
Evolved
alachlor was collected in
ethylene glycol , recovered with
C18 solid phase extraction cartridges , and analyzed by reverse - phase high - performance thin - layer chromatography with densitometry . -
pubmed
Parameters of bone metabolism ( intact
parathyroid hormone ,
serum osteocalcin and urinary
deoxypyridinoline ) and
BMD were assessed before and after the study period . -
pubmed
Nociceptin , a 17
amino acid opioid - like peptide that has an inhibitory effect on synaptic transmission in the nervous system , is involved in learning , memory , attention , and emotion and is also implicated in the perception of
pain and visual , auditory , and olfactory functions . -
pubmed
In this study , we investigated the NMR solution structure of
nociceptin in membrane - like environments (
trifluoroethanol and
SDS micelles ) and found it to have a relatively stable helix conformation from residues 4 - 17 with functionally important N - terminal residues being folded aperidoically on top of the helix . -
pubmed
In functional assays for receptor binding and
calcium flux ,
alanine - scanning variants of
nociceptin indicated that functionally important residues generally followed helix periodicity , consistent with the NMR structural model . -
pubmed
CONCLUSION : ICV injection of
STZ increases the expression of
Abeta and promotes hyerphosphorylation of
tau protein . -
pubmed
Adjustment by multiple logistic regression for
hypertension ,
triglycerides , sex , non -
HDL cholesterol ,
BMI , smoking , and
alcohol intake did not diminish the association ( OR = 1.8 , P = 0.003 ) . -
pubmed
Subjects with
HL TT exhibited trends toward increased
triglyceride and non -
HDL cholesterol levels compared with CC carriers . -
pubmed
CONCLUSIONS :
HL T+ genotypes might increase the risk of developing
diabetic nephropathy by slowing clearance of
triglyceride - rich remnant lipoproteins . -
pubmed
Hydroxy - methyl - glutaryl coenzyme A ( HMG - CoA ) reductase inhibitors have pleiotropic actions that affect many systems other than lowering blood
cholesterol concentrations . -
pubmed
These
isoprenoid moieties are required for protein prenylation and
HMG - CoA reductase inhibitors impair this function in some cells . -
pubmed
Other mechanisms have also been proposed including an increase in the free fraction of
cyclosporin and a reduction in the time that
low density lipoprotein (
LDL ) spends in blood . -
pubmed
Drug interactions between
cyclosporin and
HMG - CoA reductase inhibitors are a limiting factor to their use . -
pubmed
Pravastatin appears to be the best
HMG - CoA reductase inhibitor for organ transplant recipients because of its lesser potential to interact with
cyclosporin and hence cause
myositis , which may thus allow higher doses to be used . -
pubmed
BACKGROUND :
DE-310 is composed of the
topoisomerase - I - inhibitor
DX-8951 (
exatecan ) and a biodegradable macromolecular carrier , which are covalently linked by a peptidyl spacer . -
pubmed
For quantitation of
CoQ10 in food samples , 2 liquid chromatography ( LC ) methods with UV and mass spectrometry (
MS ) detections were developed . -
pubmed
LC /
MS analysis was performed on a LUNA
C18 3 microm , 150 x 4.6 mm column , using mobile phase consisting of
ethanol -
dioxane -
acetic acid ( 9 + 1 + 0.01 , v / v / v ) , flow rate was 0.6 mL / min , and the retention time of
CoQ10 was 4.1 + /- 0.1 min . -
pubmed
An extracellular
agarase was purified by the procedure of
ammonium sulfate precipitation , gel filtration on Sephacryl S-100HR , and ion - exchange chromatography on
diethylaminoethyl - Sepharose . -
pubmed
The
enzyme hydrolyzed the beta-1,4 - glycosidic linkages of agar , yielding
neoagarotetraose and
neoagarohexaose as the main products . -
pubmed
The optimum reaction temperature of the
agarase was 35 degrees C , with a narrow range from 30 to 45 degrees C. The
enzyme activity reached the maximum at pH 7.0 and in the presence of 2 %
NaCl . -
pubmed
There were no significant differences in total
cholesterol ,
LDL -
cholesterol and
apoB between the TRANS- and the PALM - diet . -
pubmed
HDL -
cholesterol and
apoA - I were significantly higher on the PALM - diet compared to the TRANS - diet while the ratio of
LDL- to
HDL -
cholesterol was lower , although not significantly ( P = 0.077 ) on the PALM - diet . -
pubmed
Total
cholesterol ,
LDL -
cholesterol and
apoB were significantly lower on the
PUFA - diet compared to the two other diets . -
pubmed
Triglycerides and
Lp ( a ) were not different among the three diets . -
pubmed
t - PA activity was also decreased on the
PUFA - diet compared to PALM - diet although not significantly ( P=0.07 ) . -
pubmed
Two - dimensional fluorescence correlation spectroscopy
IV : resolution of fluorescence of
tryptophan residues in
alcohol dehydrogenase and
lysozyme . -
pubmed
Generalized two - dimensional ( 2D ) fluorescence correlation spectroscopy has been used to resolve the fluorescence spectra of two
tryptophan (
Trp ) residues in
alcohol dehydrogenase and
lysozyme . -
pubmed
In each
protein , one
Trp residue is buried in a hydrophobic domain of the
protein matrix and the other
Trp residue is located at a hydrophilic domain close to the protein - water interface . -
pubmed
Fluorescence spectra of the two
Trp residues in
alcohol dehydrogenase , which are heavily overlapped in conventional one - dimensional spectra , have been successfully resolved by the 2D correlation technique . -
pubmed
In contrast , the fluorescence spectra of the two
Trp residues could not be resolved after the
alcohol dehydrogenase was denatured with
guanidine hydrochloride . -
pubmed
Fluorescence spectra of the
Trp residues in
lysozyme could not be resolved by the 2D correlation technique . -
pubmed
The differences between the two
proteins are attributed to the fact that the
Trp residue in the hydrophobic site of
lysozyme is not sufficiently protected from the quenching . -
pubmed
Temporal expression of
IL-1beta protein and mRNA in the brain after systemic LPS injection is affected by age and
estrogen . -
pubmed
Using the latter approach , we have shown that
estrogen suppresses inflammatory
cytokine expression in lesioned ovariectomized young adult females but not reproductive senescent animals . -
pubmed
However , in cultured microglia derived from either young or senescent animals ,
estrogen fails to suppress LPS - induced
cytokine expression . -
pubmed
However ,
estrogen treatment attenuated
IL-1beta expression in the olfactory bulb but not in the pituitary gland . -
pubmed
In senescent animals ,
estrogen completely suppressed
IL-1beta expression in the bulb and the pituitary gland , while placebo - replaced animals responded normally . -
pubmed
Age and hormone effects on pituitary
cytokines were also mirrored in plasma
corticosterone (
CORT ) levels , such that
estrogen treatment to senescent females attenuated LPS - induced
CORT . -
pubmed
1 - Cinnamoyl-3,11 - dihydroxymeliacarpin is a natural bioactive compound with antiviral and
nuclear factor - kappaB modulating properties . -
pubmed
CDM blocks VSV entry and the intracellular transport of
VSV - G protein , confining it to the Golgi apparatus , by pre- or
post - treatment , respectively . -
pubmed
In conclusion ,
CDM is a pleiotropic agent that not only inhibits the multiplication of DNA and RNA viruses by the same mechanism of action but also modulates the
NF - kappaB signaling pathway . -
pubmed
Elevations in mitochondrial
LPO along with decrements in enzymic antioxidants (
superoxide dismutase (
SOD ) ,
catalase (
CAT ) ,
glutathione peroxidase (
GPx ) ,
glutathione reductase (
GR ) and
glutathione - S - transferase (
GST ) ) , non - enzymic antioxidants (
reduced glutathione (
GSH ) ,
vitamin C ,
vitamin E and
vitamin A ) ,
citric acid cycle enzymes (
isocitrate dehydrogenase (
ICDH ) ,
alpha - ketoglutarate dehydrogenase (
alpha - KDH ) ,
succinate dehydrogenase (
SDH ) and
malate dehydrogenase (
MDH ) ) , and respiratory chain enzymes (
NADH dehydrogenase and
Cytochrome c oxidase ) were observed in
B ( a ) P ( 50mg / kg
body weight ) administered animals . -
pubmed
CAP ( 10mg / kg
body weight ) pretreatment decreased lung mitochondrial
LPO and augmented the activities of enzymic , non - enzymic antioxidants ,
citric acid cycle enzymes and respiratory chain enzymes to near normalcy revealing its chemoprotective function during
B ( a ) P induced
lung cancer . -
pubmed
There is no data on the influence of
PTH fractions (
CAP and
CIP ) on bone turnover and density in
hyperprolactinemia . -
pubmed
In contrast ,
PTH treatment had no effect on periosteal bone formation and minimal effects on
DABM - induced osteoinduction . -
pubmed
There was no interaction between
PTH treatment and
alcohol consumption for any of the endpoints evaluated . -
pubmed
Furthermore ,
alcohol inhibits osteoinduction and reduces periosteal and cancellous bone formation , irrespective of therapeutic
PTH administration . -
pubmed
When
sucrose was hydrolyzed in an aqueous suspension of the copoly(MA , St)-coated capsules into which
invertase was loaded , the hydrolytic reaction was initiated at pH 5 . -
pubmed
Systemic administration of
tumour necrosis factor ( TNF )- alpha induces the release of
norepinephrine in the paraventricular nucleus ( PVN ) of hypothalamus and an increase in expression of
corticotrophin - releasing factor (
CRF ) and
CRF type 1 receptors . -
pubmed
We explored the hypothesis that
CRF and
norepinephrine in PVN mediate the cardiovascular and sympathetic responses to acute systemic administration of
TNF - alpha . -
pubmed
Prazosin blocked the
TNF - alpha - induced increase in renal sympathetic nerve activity ( RSNA ) , whereas alpha - helical
CRF substantially reduced the RSNA response . -
pubmed
Conversely ,
CRF and the alpha 1 - adrenergic agonist
phenylephrine , administered ICV , both elicited increases in PVN neuronal activity , RSNA , arterial pressure and heart rate . -
pubmed
Microinjection of
CRF and
phenylephrine directly into PVN evoked smaller responses . -
pubmed
These results are consistent with the hypothesis that
norepinephrine and
CRF in the PVN mediate the cardiovascular and sympathetic responses to acute systemic administration of
TNF - alpha . -
pubmed
Lipid profile changed thus : total
cholesterol 4.97 ... 5.01mmol / l ( p = 0.7 ) ,
LDL cholesterol 2.83 ... 2.82 mmol / l mmol / l ( p = 0.9 ) ,
HDL cholesterol 1.13 ... 1.27 mmol / l ( p = 0.003 ) ,
triglycerides 1.98 ... 1.91 mmol / l ( p = 0.01 ) . -
pubmed
A novel cellulose - based polyelectrolyte (
AM - C ) containing
acylamino ( DS=0.625 ) and
carboxyl ( DS=0.148 ) groups was homogeneously synthesized from cellulose with
acrylamide in
NaOH /
urea aqueous solutions . -
pubmed
The results indicated that
AM - C could form large aggregates spontaneously in water with or without the addition of salts by the strong
hydrogen bonds and electrostatic interaction between
acylamino and
carboxyl groups . -
pubmed
Ammonium can be assimilated onto nitrogenous organic compounds by the concerted action of two enzymes that compose the
glutamine synthetase (
GS ,
EC 6.3.1.2 ) -
glutamate synthase (
Fd - GOGAT ,
EC 1.4.7.1 ;
NADH - GOGAT ,
EC 1.4.1.14 ) cycle . -
pubmed
Ammonium may also be directly incorporated into
glutamate by the
glutamate dehydrogenase (
GDH ,
EC 1.4.1.2 ) aminating reaction . -
pubmed
However , as
GDH reversibly deaminates
glutamate , its physiological role in vivo remains controversial . -
pubmed
Regarding
GS gene - specific mRNA accumulation , an organ - dependent response was also observed that contributes for the detected alteration in the
ammonium assimilatory metabolism . -
pubmed
The possible contribution of
GDH for
ammonia assimilation was also investigated . -
pubmed
Adjusted models indicated a modest risk among
AZA patients for graft loss (
AHR = 1.14 , 95 % CI 1.07 - 1.20 ) ; however , this was not apparent among
AZA patients also treated with
tacrolimus (
AHR = 0.97 , 95 % CI 0.85 - 1.11 ] . -
pubmed
Therefore , it was intuitive to obtain the sequence of the antitumor activity of four anticancer drugs without data processing as follows :
mitoxantrone (
MIT ) & gt ;
pirarubicin (
PIR ) & gt ;
daunorubicin (
DAU ) & gt ;
doxorubicin (
DOX ) by
RLS screening spectra . -
pubmed
Moreover , the apparent equilibrium constant ( K ) was 1.23 x 10(4 ) , 2.22 x 10(4 ) , 4.66 x 10(4 ) L / mol for
DOX ,
DAU , and
PIR , respectively . -
pubmed
The inhibitory concentration 50 ( IC50 ) was 0.148 , 0.102 , 0.025 , 0.013 micromol / L for
DOX ,
DAU ,
PIR , MIT , respectively . -
pubmed
Therefore , the antitumor effect of four drugs was as follows :
MIT & gt ;
PIR & gt ;
DAU & gt ;
DOX , which was in good agreement with the result obtained from
RLS screening assays . -
pubmed
1 and 2 are isomorphous , they possess a three - dimensional architecture constructed from
Ln ( 3 + ) ions bridged by
oxalate anions and two types of
1H-6 - Opy-3 - CO ( 2 )(- ) bridges , showing a three -
nodal ( 4,5)-connected topology ( 3.4(2).5(2).6(3).7.8)(2)(3.5(3).6(2))(2)(3(2).6.7(2).8 ) or a simplified uninodal 6 - connected topology ( 3(3).4(6).5(5).6 ) , both topologies are completely new ; while only one type of
1H-6 - Opy-3 - CO ( 2 )(- ) bridge is used to construct the two - dimensional layer networks of 3 and 4 besides
oxalate bridges , both complexes 3 and 4 are isostructural , exhibiting the honeycomb topology 6(3 ) . -
pubmed
Regional
Tc 99m - DTPA GFR of both affected kidney and nonaffected kidney showed no significant differences perioperatively (
6.3 + - 1.8 to 6.1 + - 1.9 ml / min per 1.73 m2 ; p = 0.641 ; 6.6 + - 1.9 to 7.1 + - 2.0 ml / min per 1.73 m2 ; p = 0.200 ) . -
pubmed
Cytochrome c nitrite reductase ,
NrfA , catalyzes the six - electron reduction of
nitrite ,
NO ( 2 )(- ) , to
ammonium ,
NH ( 4 )(+ ) , as the final enzymatic step in the dissimilatory metabolic pathway of
nitrite ammonification within the biogeochemical
nitrogen cycle . -
pubmed
NrfA is a 55 - 65kDa protein that binds five c - type
heme groups via
thioether bonds to the
cysteines of conserved CXXCH
heme attachment motifs . -
pubmed
While no intermediates of the reaction are released ,
NrfA is able to reduce various other
nitrogen oxides such as
nitric oxide (
NO ) ,
hydroxylamine (
H ( 2 ) NOH ) , and
nitrous oxide (
N ( 2 ) O ) , but notably also
sulfite , providing the only known direct link between the
nitrogen and
sulfur cycles . -
pubmed
In many enterobacterial species ,
NrfA is linked to the
menaquinol pool in the cytoplasmic membrane through a soluble electron carrier ,
NrfB , that in turn interacts with a membrane - integral
quinol dehydrogenase ,
NrfCD . -
pubmed
AIM OF THE STUDY : Reperfusion following
cerebral ischemia leads to excessive production of reactive
oxygen species ( ROS ) and consumption of endogenous antioxidants . -
pubmed
Copper ,
zinc - superoxide dismutase (
SOD1 ) is one of the major defensive mechanisms by which cells counteract the deleterious effects of ROS after
ischemia . -
pubmed
Our recent research provides novel molecular and functional evidence that
indoxyl sulfate , an anionic uremic toxin , undergoes efflux transport at the BBB via
OAT3 and
creatinine , a uremic
guanidino compound , undergoes efflux transport at the BCSFB via
OCT3 . -
pubmed
EDTA -
imipenem disc synergy tests , and
polymerase chain reaction amplification and sequencing were performed to detect the presence of
metallo - beta - lactamases (
MBLs ) . -
pubmed
Na delivery and
ENaC mediate flow regulation of collecting duct
endothelin-1 production . -
pubmed
Collecting duct (
CD )
endothelin-1 (
ET-1 ) is an important autocrine inhibitor of
Na and water transport . -
pubmed
CD
ET-1 production is stimulated by extracellular fluid volume expansion and tubule fluid flow , suggesting a mechanism coupling CD
Na delivery and
ET-1 synthesis . -
pubmed
Flow with 300 mosmol / l
NaCl increased
ET-1 mRNA to 65 % above that observed under static conditions . -
pubmed
Increasing perfusate osmolarity to 450 mosmol / l with
NaCl or
Na acetate increased
ET-1 mRNA to ~184 % compared with no flow , which was not observed when osmolarity was increased using
mannitol or
urea . -
pubmed
Inhibition of
epithelial Na channel (
ENaC ) with
amiloride or
benzamil abolished the flow response , suggesting involvement of
ENaC in flow - regulated
ET-1 synthesis . -
pubmed
Since
Ca ( 2 + ) enhances CD
ET-1 production , the involvement of plasma membrane and mitochondrial
Na / Ca ( 2 + ) exchangers (
NCX ) was assessed . -
pubmed
SEA0400 and
KB - R7943 , plasma membrane
NCX inhibitors , did not affect the flow response . -
pubmed
However ,
CGP37157 , a mitochondrial
NCX inhibitor , abolished the response . -
pubmed
In summary , the current study indicates that increased
Na delivery , leading to
ENaC - mediated
Na entry and mitochondrial
NCX activity , is involved in flow - stimulated CD
ET-1 synthesis . -
pubmed
Dermatological adverse effects during genotype-1
hepatitis C treatment with the
protease inhibitors
telaprevir and
boceprevir . -
pubmed
Of the three compounds ,
apigenin-8 - C - beta - D -( 2 "- O - alpha - L - rhamnosyl ) glucopyranoside was identified based on its UV ,
MS , and NMR spectra . -
pubmed
PYR / RCAR receptors contribute to
ozone- , reduced air humidity- , darkness- , and
CO2 - induced stomatal regulation . -
pubmed
Rapid stomatal closure induced by changes in the environment , such as elevation of
CO2 , reduction of air humidity , darkness , and
pulses of the air pollutant
ozone (
O3 ) , involves the
SLOW ANION CHANNEL1 (
SLAC1 ) . -
pubmed
OST1 activation is controlled through
abscisic acid (
ABA)-induced inhibition of
type 2 protein phosphatases (
PP2C ) by
PYRABACTIN RESISTANCE / REGULATORY COMPONENTS OF ABA RECEPTOR (
PYR /
RCAR ) receptor proteins . -
pubmed
The stomatal conductance values varied severalfold among the studied mutants , indicating that basal
ABA signaling through
PYR /
RCAR receptors plays a fundamental role in controlling whole - plant water loss through stomata . -
pubmed
PYR /
RCAR - dependent inhibition of
PP2Cs was clearly required for rapid stomatal regulation in response to darkness , reduced air humidity , and
O3 . -
pubmed
Although a rapid stomatal response to elevated
CO2 was evident in all but
slac1 and
ost1 mutants , the
bicarbonate - induced activation of S - type anion channels was reduced in the dominant active
PP2C mutants
abi1 - 1 and
abi2 - 1 . -
pubmed
Thus , we show that
PYR /
RCAR receptors play an important role for the whole - plant stomatal adjustments and responses to low humidity , darkness , and
O3 and are involved in responses to elevated
CO2 . -
pubmed
Role of the central
arginine R133 toward the ion selectivity of the
phosphate specific channel
OprP : effects of charge and solvation . -
pubmed
A detailed investigation of the structure - function relationship of
OprP is inevitable to decipher the anion and
phosphate selectivity of this
porin in particular and to broaden the present understanding of the ion selectivity of different channels . -
pubmed
To this end we investigated the role of the central
arginine of
OprP , R133 , in terms of its effects in selectivity and ion transport properties of the pore . -
pubmed
For these mutants , the change in
phosphate binding specificity , ion conduction , and anion selectivity was determined and compared to previous molecular dynamic calculations and electrophysiological measurements with wild - type
OprP. -
pubmed
Herein , we demonstrate that ionic supramolecular bonds , introduced by infiltration of divalent
Cu ( 2 + ) ions , allow efficient stabilization of the mechanical properties of self - assembled water - borne nacre - mimetics based on sustainable
sodium carboxymethylcellulose (
Na(+)CMC ) and natural
sodium montmorillonite nanoclay (
Na(+)MTM ) against high humidity ( 95 %
RH ) . -
pubmed
To illustrate the interaction between
CCR5 and microglia in the CNS , we used a model of excitotoxicity , and demonstrate the intimate involvement of
CCR5 in
neuron injury and
inflammation attendant to
kainic acid ( KA)-induced
neurotoxicity . -
pubmed
Acquired
factor VIII inhibitor and positive lupus anticoagulant testing on
silica clotting time . -
pubmed
AF can be terminated in 3 modes : ( 1 ) directly into
sinus rhythm (
SR ) ; ( 2 ) evolving into regular
atrial tachycardia (
AT ) and subsequently into
SR ; and ( 3 ) after direct current ( DC ) cardioversion if
AF persists . -
pubmed
CONCLUSIONS : The use of this amount of
pentoxifylline did not modify the serum levels of
hepcidin in this population . -
pubmed
Epigallocatechin-3 - gallate (
EGCG ) exerts anti - inflammatory properties on immune cells and binds to
CD4 molecules . -
pubmed
CD4 blockade did not suppress the increase in
IFN - gamma - induced
STAT1 activation in CD4(+ ) T cells by
EGCG . -
pubmed
Interestingly ,
EGCG promoted apoptosis of CD4(+ ) T cells treated with
IFN - gamma . -
pubmed
Moreover ,
EGCG alleviates
CD4(+ )
CD45RB(hi )
CD25(- ) T cell transfer induced
colitis with less accumulation of
CD4(+ ) T cells in the colon . -
pubmed
Our findings suggest a novel role of
EGCG in regulating
IFN - gamma signaling and controlling
inflammation . -
pubmed
The Effects of a
GLP-1 Analog on
Glucose Homeostasis in
Type 2 Diabetes Mellitus Quantified by an Integrated
Glucose Insulin Model . -
pubmed
The effect of
liraglutide was incorporated in the model by including a stimulatory effect on
insulin secretion . -
pubmed
As other
GLP-1 receptor agonists have similar modes of action , it is believed that the model can also be used to describe the effect of other receptor agonists on
glucose homeostasis . -
pubmed
An aim of this study is to investigate whether the combination of US - targeted microbubble destruction ( UTMD ) and
polyethylenimine (
PEI ) ( UTMD /
PEI ) can mediate even
greater gene transfection efficiency than UTMD alone and to optimize ultrasonic irradiation parameters . -
pubmed
UTMD /
PEI , combined with
albumin - coated microbubbles , warrants further investigation for therapeutic gene delivery . -
pubmed
parareesei
Tparo7 gene , encoding a
chorismate mutase (
CM ) , a
shikimate pathway branch point leading to the production of
aromatic amino acids , which are not only essential components of protein synthesis but also the precursors of a wide range of secondary metabolites . -
pubmed
In addition , the plants turned yellowish and were defective in
jasmonic acid- and
ethylene - regulated signaling pathways which was seen by expression analysis of
lipoxygenase 1 (
LOX1 ) ,
ethylene - insensitive protein 2 (
EIN2 ) and
pathogenesis - related protein 1 (
PR-1 ) genes . -
pubmed
These brackets along with stainless steel (
SS ) or
nickel -
titanium (
NiTi ) orthodontic wires ( 0.016 " , round ) were immersed in Oral - B mouthwash containing 0.05 %
sodium fluoride for 28 days . -
pubmed
The electric potential (
EP ) difference of each bracket - wire couple was measured with a Saturated Calomel Reference Electrode (
Ag /
AgCl saturated with
KCl ) via a voltmeter . -
pubmed
The
copper ions released from specimens with
NiTi wire were greater than those of samples containing
SS wire . -
pubmed
Corrosion rate of brackets coupled with
NiTi wires was higher than that of brackets coupled with
SS wires . -
pubmed
To evaluate whether
ZMP accumulated during
MTX therapy , we measured the concentration of
ZMP in erythrocytes and the concentration of its dephosphorylated derivative
5 - aminoimidazole-4 - carboxamide-1 - beta - d - ribofuranoside (
AICAR ) in urine using liquid chromatography mass spectrometry ( LC -
MS /
MS ) . -
pubmed
To assess
glucose homeostasis , we determined the concentration of
glycated haemoglobin ( HbA1c ) and homeostasis model assessment of
insulin resistance [ HOMA - IR : fasting
glucose ( mmol / L ) x fasting
insulin ( muU / mL)/22.5 ] . -
pubmed
Herein we report a novel
tumor vascular - targeting multidrug delivery system using mesoporous
silica nanoparticles as carrier to co - load an
antiangiogenic agent (
combretastatin A4 ) and a chemotherapeutic drug (
doxorubicin ) and conjugate with targeting molecules ( iRGD peptide ) for combined anti - angiogenesis and chemotherapy . -
pubmed
Wheel running increased the percentage of
BrdU - labeled cells expressing
DCX in the SVZ , whereas
CORT treatment decreased this percentage . -
pubmed
In addition ,
CORT can suppress the cell differentiation within the SVZ , and this was alleviated by wheel running as indicated by the double labeling of
BrdU and
DCX . -
pubmed
Serum
FGF23 , 1 ,
25 - dihydroxy vitamin D3 and
parathyroid hormone were measured by enzyme - linked immunosorbent assay . -
pubmed
FGF23 remained significantly associated with serum
creatinine ( P = 0.046 ) and estimated
glomerular filtration rate ( P = 0.034 ) after full adjustment . -
pubmed
Cytoplasmic and nuclear extracts were isolated from fresh cerebrum and cerebellum and used to determine
thioredoxin (
Trx ) and
glutathione (
GSH ) levels , as well as
thioredoxin reductase (
TrxR ) and
glutathione peroxidase (
GPx ) activities . -
pubmed
MeHg - induced antioxidant response was not uniform for all the analyzed antioxidant molecules , and sexual dimorphism in response to
MeHg treatment was evident for
TrxR ,
Trx and
GPx . -
pubmed
The study demonstrates for the first time sex - and structure - specific changes in the response of the
thioredoxin system to
MeHg neurotoxicity and suggests that these differences in antioxidant responses might impart differential susceptibility to developmental
MeHg exposure . -
pubmed
One such efficacious therapy is the anti -
CD38 monoclonal antibody ,
daratumumab (
Dara ) . -
pubmed
A free software for the calculation of
T2 * values for
iron overload assessment . -
pubmed
Background
Iron overload assessment with magnetic resonance imaging ( MRI ) using
T2 * has become a key diagnostic method in the management of many diseases . -
pubmed
This mutation substitutes a highly conserved glutamic to arginine at the position of the 47th
amino acid which was shown to be located on the flank of the pleated sheet domain in
PATE1 protein by the 3D model given by the Protein Model Portal ( PMP ) . -
pubmed
Independent activation of ion conduction pores in the double - barreled
calcium - activated
chloride channel
TMEM16A. -
pubmed
The
TMEM16 proteins constitute a family of membrane proteins with unusual functional breadth , including lipid scramblases and
Cl- channels . -
pubmed
Members of both these branches are activated by
Ca2 + , acting from the intracellular side , and probably share a common architecture , which was defined in the recent structure of the lipid scramblase
nhTMEM16 . -
pubmed
The structural features of subunits and the arrangement of
Ca2 + -binding sites in
nhTMEM16 suggest that the dimeric protein harbors two locations for catalysis that are independent with respect to both activation and lipid conduction . -
pubmed
Here , we ask whether a similar independence is observed in the
Ca2 + -activated
Cl- channel
TMEM16A. -
pubmed
Our results strongly suggest that
TMEM16A contains two ion conduction pores that are independently activated by
Ca2 + binding to sites that are embedded within the transmembrane part of each subunit . -
pubmed
The established correlation between the extinction horizon and lowest
CAMP basalts allows this sedimentary
Hg excursion to be stratigraphically tied to a specific flood basalt unit , strengthening the case for volcanic
Hg as the driver of sedimentary
Hg /
TOC spikes . -
pubmed
Additional
Hg / TOC peaks are also documented between the extinction horizon and the Triassic - Jurassic boundary ( separated by ~200 ky ) , supporting pulsatory intensity of
CAMP volcanism across the entire province and providing direct evidence for episodic volatile release during the initial stages of
CAMP emplacement . -
pubmed
The antioxidant action of
MRPs was also evaluated using a
peroxide - scavenging test based on
crocin bleaching . -
pubmed
Our PEGylated MMNPs are highly stable in both
phosphate - buffered saline (
PBS ) and in cell culture media and exhibit no
cytotoxicity up to at least 100 mug mL-1 concentrations . -
pubmed
Although liver enzymes ,
bilirubin and
albumin demonstrated a circadian pattern , it was not associated with
fatigue . -
pubmed
Together with the light subunit
LAT1 ,
CD98hc constitutes a heterodimeric transmembrane
amino acid transporter . -
pubmed
RESULTS : High expression levels of
CD98hc resulted in an increase in
mTOR pathway activation ,
amino acid metabolism , and DNA repair as well as downregulation of oxidative stress and autophagy . -
pubmed
Transcription of the desiccation - related genes
proV ,
STM1494 ,
kdpA , and
otsB in response to
paenibacterin treatment was investigated using reverse transcription - quantitative PCR . -
pubmed
Furthermore ,
DEHP induced dysregulation of oxidative stress markers by increasing
malondialdehyde (
MDA ) content and decreasing
superoxide dismutase (
SOD ) ,
glutathione peroxidase (
GPx ) , and
catalase (
CAT ) activities . -
pubmed
Low concentration of
DEHP ( & lt;=250 mg / kg ) exposure suppressed
nuclear factor - E2 - related factor 2 (
Nrf2 ) signaling pathway , while high concentration of
DEHP ( & gt;=500 mg / kg ) exposure activated
Nrf2 - mediated defense response . -
pubmed
DEHP induced splenic oxidative stress via interfering
Nrf2 signal pathway and altering the transcription of its downstream genes . -
pubmed
Individual free
fatty acids have unique associations with inflammatory biomarkers ,
insulin resistance and
insulin secretion in healthy and gestational
diabetic pregnant women . -
pubmed
Objective : We investigated the relationships of maternal circulating individual free
fatty acids ( FFA ) with
insulin resistance ,
insulin secretion and inflammatory biomarkers during mid - pregnancy . -
pubmed
For example , women who had elevated
DGLA ( highest quartile ) were twice as ( adjusted OR 2.06 , 95 % CI 1.42 to 2.98 ) likely to have higher
interleukin ( IL )- 8 ( p<0.0001 ) levels . -
pubmed
Conversely , women with high
palmitoleic , oleic , and linolenic acid levels had reduced odds ( & gt;=2 - fold , p<0.01 to p<0.001 ) for having higher
IL-8 ,
IL-6 or
tumor necrosis factor - alpha levels . -
pubmed
Conclusion : Our results suggest that maternal individual
FFAs uniquely affect
insulin resistance and secretion . -
pubmed
CeO2NPs relieve radiofrequency radiation , improve
testosterone synthesis , and
clock gene expression in Leydig cells by enhancing antioxidation . -
pubmed
The cells were collected to determine the antioxidant indices (
catalase [
CAT ] ,
malondialdehyde [
MDA ] , and total antioxidant capacity [ T - AOC ] ) , and the mRNA expression of the
testosterone synthase genes (
Star ,
Cyp11a1 , and
Hsd-3beta ) and
clock genes (
Clock ,
Bmal1 , and
Roralpha ) . -
pubmed
Cells exposed to RF alone showed reduced levels of
testosterone , T - AOC , and
CAT activities , increased
MDA content , and the downregulated genes expression of
Star ,
Cyp11a1 ,
Hsd-3beta ,
Clock ,
Bmal1 , and
Roralpha . -
pubmed
Pretreatment of the cells with 128 mug / mL CeO2NPs for 24 hrs followed by RF exposure significantly increased
testosterone synthesis , upregulated the expression of the
testosterone synthase and
clock genes , and increased the resistance to oxidative damage in Leydig cells compared with those in cells exposed to RF alone . -
pubmed
Conclusion : Exposure to 1,800 MHz RF had adverse effects on
testosterone synthesis , antioxidant levels , and
clock gene expression in primary Leydig cells . -
pubmed
Pretreatment with CeO2NPs prevented the adverse effects on
testosterone synthesis induced by RF exposure by regulating their antioxidant capacity and
clock gene expression in vitro . -
pubmed
Distinct roles of an ionic interaction holding an alpha - helix with catalytic
Asp and a beta - strand with catalytic
His in a hyperthermophilic
esterase EstE1 and a mesophilic
esterase rPPE . -
pubmed
An ionic interaction that holds an alpha - helix and a beta - strand on which catalytic
Asp and
His residues are located , respectively , is conserved in a hyperthermophilic
esterase EstE1 ( optimum temperature 70 C ) and a mesophilic
esterase rPPE ( optimum temperature 50 C ) . -
pubmed
Ala substitutions caused a 5 - 10 C decrease in the optimum temperature of both
EstE1 and
rPPE mutants . -
pubmed
The results of this study suggest that the ionic interaction between the alpha - helix with catalytic
Asp and the beta - strand with catalytic
His plays an important role in the active - site conformation of
EstE1 and
rPPE , with larger effects on the conformational flexibility of hyperthermophilic
EstE1 and the thermal stability of mesophilic
rPPE . -
pubmed
Genetic down - regulation of
CHRM4 or pharmacologic inhibition of
CHRM4 using the selective antagonist
PD102807 promoted BFU - E self - renewal , whereas deletion of
Chrm4 increased erythroid cell production under stress conditions in vivo . -
pubmed
The effects of
muscarinic receptor antagonism on promoting expansion of BFU - Es were mediated by
cyclic AMP induction of the
transcription factor CREB , whose targets up - regulated key regulators of BFU - E self - renewal . -
pubmed
Herein ,
cobalt phosphides anchored or embedded in spirulina - derived porous
N - doped carbon matrix (
Co 2 P -
NC ) are fabricated by two - step hydrothermal treatment and carbonization method , which cleverly utilized the intrinsic
carbon ,
nitrogen and
phosphorus of biomass as the source of C , N and P respectively . -
pubmed
However , the presence of
nitrite (
NO2 ) , a central intermediate of the
nitrogen cycle , interferes with the electrochemical determination of the
EAC . -
pubmed
In addition to this ionotropic function , non - ionotropic signalling can be initiated through the exclusive binding of
glycine or of
glutamate to the
NMDAR . -
pubmed
Solution structures of
copper ( i / ii ) complexes were calculated using density functional theory ( DFT ) and validated by both NMR and
EPR spectroscopy . -
pubmed
The
tert - butyl hydroperoxide (
t - BHP ) exposure aging model bone mesenchymal stem cells ( BMSCs ) were reverted by using a poly - hybrid scaffold ( PS ) , which is a
carbon nanotube ( CNT ) coated
polycaprolactone (
PCL ) and
polylactic acid (
PLA ) scaffold , combined with
insulin - like growth factor-1 (
IGF ) . -
pubmed
Changes in the sensitivity of the umbilical cord blood vessels to BRS , depending on the saturation with
oxygen , have been emphasised , and the role and participation of
kinins in closing up umbilical cord blood vessels immediately following labour have been discussed . -
pubmed
The
enzyme was shown to be homogenous by three different criteria :
polyacrylamide disc gel electrophoresis ,
SDS polyacrylamide gel electrophoresis and analytical ultracentrifugation . -
pubmed
The molecular weight of the
alpha - amylase was determined by three different methods : sedimentation velocity - diffusion , conventional sedimentation equilibrium and
SDS polyacrylamide gel electrophoresis and was found to be 57,850 ; 50,100 and 53,200 g mole-1 , respectively ( average value 53,700 ) . -
pubmed
The
amino acid composition of the
enzyme was determined and compared with those of
alpha - amylases from various other sources . -
pubmed
[
Aminoacids in the
keratin of skin , hair and nails in
psoriasis cases ( author 's transl ) ] . -
pubmed
Morphine in doses of 2.5 - 10 mg / kg definitely inhibited
SI and
SII potentials evoked by
CTP stimulation . -
pubmed
Morphine also inhibited
SII potentials evoked by ITP stimulation , while it rather enhanced
SI potentials evoked by ITP stimulation . -
pubmed
Pentobarbital sodium in doses of 4 - 16 mg / kg tended to inhibit
SI potentials , but showed no effect or rather an enhancement on
SII potentials evoked by
CTP stimulation . -
pubmed
Pentobarbital sodium enhanced
SI and
SII potentials evoked by ITP stimulation . -
pubmed
In a large dose of 32 mg / kg ,
pentobarbital sodium inhibited
SI and
SII potentials evoked by ITP and
CTP stimulations . -
pubmed
The results suggest that
SII is more closely related to the analgesia due to
morphine than is
SI . -
pubmed
The sensitivity of the
Na +/ H + exchanger to the oxidant ,
hydrogen peroxide (
H2O2 ) and the protection by the antioxidant
GSH suggests a possible role for the
Na+/H+ exchange mechanism in the formation of
cataracts . -
pubmed
Mutations in
Met168 highly decreased the specificity constant of the
enzyme for
penicillin G ,
penicillin V and
phenylacetyl-4 - aminobenzoic acid and the catalytic constant kcat for
phenylacetyl-4 - aminobenzoic acid . -
pubmed
A putative
penicillin - binding domain was located in
penicillin acylase by sequence homology with other
penicillin - recognizing enzymes . -
pubmed
Lys374 and
His481 , the conserved
amino acid residues that are essential for catalysis in these enzymes , can be changed in
penicillin acylase with no changes to the kcat and
phenylmethylsulphonyl fluoride reactivity , but change the Km . -
pubmed
The reported results might be used to alter the substrate specificity of
penicillin acylase in order to hydrolyse substrates of industrial significance other than
penicillins . -
pubmed
A small group of
lectins was identified , all with a main binding requirement of
N - acetylgalactosamine that bound to all B lymphocytes but only to activated T lymphocytes expressing the
interleukin-2 ( IL-2 ) receptor ( as shown by staining with the
monoclonal antibody OX39 ) . -
pubmed
With the knowledge of the binding requirements of these
lectins , a structure can be deduced for the
carbohydrate moiety which appears on T lymphocytes when activated . -
pubmed
In the present study , we determined how the
arachidonic acid cascade , mainly
thromboxane A2 (
TXA2 ) , is related to
ET-1 and
ET-3 using
indomethacin (
INDO ) , which inhibits the biosynthesis of
prostaglandin (
PG ) , and
OKY-046 , a selective inhibitor of
TXA2 synthesis . -
pubmed
While
INDO failed to inhibit the slight decrease in hepatic blood flow induced by
ET-1 , it significantly inhibited the marked decrease in hepatic blood flow elicited by
ET-3 . -
pubmed
In the next series of experiments ,
ET-1 and
ET-3 were administered after administration of 20 mg / kg of
OKY-046 . -
pubmed
OKY-046 showed no effects in animals treated with
ET-1 , as in those pre - treated with
INDO , while it significantly inhibited the decreases in hepatic blood flow induced by
ET-3 . -
pubmed
These findings suggest that
ET-1 decreases hepatic blood flow due to its direct effects although to a lesser extent than
ET-3 , while
ET-3 does so due not only to its direct effects but also to
TXA2 - mediated effects . -
pubmed
Cycloheximide blocks glucocorticoid - induced transcription of both the -353CAT and -239CAT reporter genes ( nucleotides -447 to -12 and nucleotides -239 to -12 of
hGSTYBX , respectively , ligated to a
CAT reporter gene ) ; therefore , our observations support previous results suggesting that
hGSTYBX induction by glucocorticoids is a secondary response . -
pubmed
The treatment of mesangial
IgA nephropathy with
cyclophosphamide ,
dipyridamole and
warfarin . -
pubmed
Cholesterol crystals and
IgE - containing immune complexes in
rheumatoid pericarditis . -
pubmed
Examination of the pericardial fluid revealed the presence of
cholesterol crystals and
IgE - containing immune complexes . -
pubmed
Captopril ( 8 , 16 , 32 mg / kg / day ,
IP ) induced a reversal of escape deficits but did not affect significantly the motor activity , suggesting that this effect was not due to motor stimulation . -
pubmed
This antidepressant - like activity was comparable to that of
imipramine ( 16 , 32 mg / kg / day ,
IP ) . -
pubmed
Naloxone ( 0.5 ; 1 mg / kg ,
IP ) blocked the effect of
captopril ( 16 mg / kg ,
IP ) in this test . -
pubmed
Pyridostigmine partially restores the
GH responsiveness to
GHRH in normal aging . -
pubmed
The
GH response to
pyridostigmine alone was also examined . -
pubmed
Group I cases were given anti - schistosomal drugs (
oxamniquine and
praziquantel ) , group
II cases were given anti - schistosomal drugs plus
prednisolone , and group III cases were given anti - schistosomal drugs plus
cyclosporine . -
pubmed
Direct observation of the
enzyme - intermediate complex of
5 - enolpyruvylshikimate-3 - phosphate synthase by
13C NMR spectroscopy . -
pubmed
The interaction of
5 - enolpyruvylshikimate-3 - phosphate ( EPSP ) synthase ,
4,5 - dideoxyshikimate 3 - phosphate (
ddS3P ) , and
[ 2 - 13C ]-and
[ 3 - 13C ] phosphoenolpyruvate (
PEP ) has been examined by
13C NMR spectroscopy . -
pubmed
Although no resonances due to a dead - end intermediate complex could be detected , an
enzyme active site specific formation of
pyruvate was observed . -
pubmed
The interaction of
EPSP synthase with
shikimate 3 - phosphate (
S3P ) and
[ 2 - 13C ]- or
[ 3 - 13C ] PEP has been examined by
13C NMR spectroscopy . -
pubmed
The resonance at 164.8 ppm has been assigned to
enzyme - bound
EPSP . -
pubmed
The resonance at 88.9 ppm has been assigned to
enzyme - bound
EPSP . -
pubmed
Neither
mesaton nor
isadrine altered the MP after blocking of the
Na +/ K + pump . -
pubmed
Catecholamines seem to exert a direct alpha - adrenomimetic and an indirect beta - adrenomimetic reversible blocking effect upon the activity of the
Na +/ K +- ionic pump . -
pubmed
The DNA stain
Hoechst 33258 binds preferentially to
AT base pairs . -
pubmed
We investigated the bioactivities of the recombinant DNA - derived
methionyl 20 K
hGH ( 20K - Met -
hGH ) and methionyl
hGH ( 22K - Met -
hGH ) . -
pubmed
The receptors for
insulin and
insulin - like growth factor I (
IGF - I ) are closely related molecules , with an extracellular binding domain and an intracellular
tyrosine kinase domain . -
pubmed
Stimulation of both
[ 3H ] glucose incorporation into glycogen and
alpha -[ 3H ] aminoisobutyric acid uptake by
insulin was half - maximal at concentrations of 1 - 5 nmol / L. These effects were blocked by the
insulin receptor monoclonal antibody MA-10 , but not by the
IGF - I receptor antibody alpha IR-3 . -
pubmed
Stimulation of both functions by
IGF - I was half - maximal at concentrations of 1 - 5 nmol / L , and these effects were inhibited by
alpha IR-3 , but not by
MA-10 . -
pubmed
The first complex leaves neither a
DNase footprint nor is the DNA protected from methylation by
dimethyl sulfate . -
pubmed
Vanadate ,
lanthanum ,
N - ethylmaleimide and
calmodulin inhibitor
R24571 inhibited this pump activity . -
pubmed
Antiinflammatory actions of
cyclooxygenase inhibitors may be related to inhibition of the synthesis and release of
prostaglandins and
thromboxane or to nonspecific actions of particular drugs . -
pubmed
The role of
cyclooxygenase products of
arachidonic acid was studied in two leukocyte functions , free radical release and aggregation , after complement activation . -
pubmed
In the presence of a
protein kinase inhibitor , neither
PMA nor any of the
cytokines used are able to induce
TNF - alpha mRNA accumulation , indicating that
protein kinases may be crucially involved in signal transduction leading to activation of the
TNF - alpha gene . -
pubmed
This suggests that
molybdate does not prevent receptor transformation , but rather inhibits disaggregation of the
8S oligomer into the
4S monomer . -
pubmed
While
IFN - beta mRNA rapidly decays after reaching a maximum 2 - 4 h after induction , this 12S RNA is stably maintained in the fibroblast cell for more than 16 h. Contrary to
IFN - beta mRNA , the level of the 12S RNA is not further elevated by superinduction conditions (
cycloheximide treatment ) during poly(rI).poly(rC ) induction . -
pubmed
Interactions of
pig brain cytosolic sialidase with
gangliosides . -
pubmed
Reversible inactivation of cytosolic
sialidase requires the presence of homogeneous micelles of
GT1b or of mixed micelles ( for instance
Triton X-100 and
GT1b ) with a high
GT1b content . -
pubmed
Triton X-100 /
ganglioside mixed micelles with a molar ratio above 50 , as well as small unilamellar vesicles of egg yolk
lecithin and
GT1b ( 7 - 15 mol% ) , did not inactivate the enzyme at all ; on the contrary these forms of
ganglioside dispersion behaved as excellent substrates for the
enzyme . -
pubmed
It is to be concluded that under in vitro conditions the ability of
ganglioside to interact with cytosolic
sialidase , giving rise to catalytically inactive complexes or to Michaelis - Menten enzyme - substrate complexes , depends on the supramolecular organization of the
ganglioside molecules . -
pubmed
Short term effects of
insulin on the exocrine pancreas in vitro , using isolated pancreatic acini , like stimulation of
glucose- and
aminoacid uptake , stimulation of proteinsynthesis , phosphorylation of certain proteins and stimulation of
CCK induced
amylase release are discussed . -
pubmed
beta - Endorphin ( 2 microgram / kg
IP ) has no effect on acquisition , but , when given prior to test sessions , facilitates retrieval of the two tasks . -
pubmed
In addition to these effects , the pretraining administration of
beta - endorphin disrupts , and that of
naloxone facilitates retention of the two tasks . -
pubmed
[ Pathogenetic aspects of the effect of thyroid hormones on
glucose homeostasis : role of the
insulin - producing apparatus of the pancreas ] . -
pubmed
Maximum stimulation was dependent on the concentration of
NAD + used in the assay ; an increase in Km value of
NAD + was observed to parallel the increase in maximal velocity with increasing
Mg2 + concentration , indicating that alterations in the catalytic properties of the
E2 isozyme occur in the presence of
Mg2 + . -
pubmed
The presteady state burst of
NADH product was observed to decrease in the presence of
Mg2 + , suggesting that the rate - limiting step of the
dehydrogenase reaction is altered by
Mg2 + . -
pubmed
No evidence for
Mg2 + -induced alterations in the molecular weight properties of the
E2 isozyme was observed using gel filtration column chromatography and fluorescence polarization techniques . -
pubmed
Analysis of the incorporated
sugars after hydrolysis of the
glycoprotein showed that
glucosamine and
mannose were utilized in biosynthesis of the
carbohydrate moiety directly whereas
galactose was converted possibly to other intermediates before being incorporated into the
antigen . -
pubmed
Tunicamycin completely prevented the incorporation of the
radiolabeled sugars into the surface
glycoprotein . -
pubmed
Ovarian concentrations of total
CoA , free
carnitine and
acetylcarnitine increased 3 - fold on
gonadotropin stimulation , whereas there was no marked change in total
CoA and acid - soluble
carnitine concentrations in the other organs . -
pubmed
The prepubertal pattern of
gonadotropin release can , in part , be explained on the basis of the development of pituitary
GnRH sensitivity , which occurs independently of photoperiod . -
pubmed
Compactin (
ML-236B ) and the related compound ,
mevinolin , are competitive inhibitors of
3 - hydroxy-3 - methylglutaryl coenzyme A reductase (
HMG CoA reductase ) , the rate - controlling enzyme in
cholesterol synthesis . -
pubmed
By blocking
mevalonate synthesis ,
mevinolin activates this regulatory mechanism , and this in turn causes an increase in hepatic
HMG CoA reductase . -
pubmed
The ability to suppress the elevated
HMG CoA reductase with
mevalonate may prove useful in potentiating the effectiveness of
mevinolin as a hypocholesterolemic agent . -
pubmed
N - CAM has an unusual
carbohydrate content and structure , is polydisperse in solution , and is associated with proteolytic activity leading to its spontaneous cleavage . -
pubmed
Histochemical methods demonstrated the recovery of the glycogen content , the activity of
succinate dehydrogenase and
alpha - glycerophosphate dehydrogenase as well as increased fluorescence of catecholaminergic nerves in short - term ischemized myocardium ( experimental
angina pectoris ) after stimulation of the carotid
sinus nerve . -
pubmed
The role of a
phosphatidylcholine - specific
phospholipase C in the production of
diacylglycerol for
nitric oxide synthesis in macrophages activated by
IFN - gamma and LPS . -
pubmed
Through analysis of
diacylglycerol (
DAG ) synthesis and
choline metabolism in activated macrophages , direct evidence is provided that
NO synthesis involves the activation of an unusual
phosphatidylcholine - specific phospholipase C (
PC - PLC ) and not a
phosphatidylinositol - specific phospholipase C (
PI - PLC ) or
phospholipase D (
PLD ) . -
pubmed
Design , synthesis and characterization of photoreactive
benzophenone - containing analogs of
parathyroid hormone . -
pubmed
Parathyroid hormone (
PTH ) regulates
calcium and
phosphate metabolism through a
G - protein - coupled receptor which is shared with
PTH - related protein (
PTHrP ) . -
pubmed
Our approach to studying the nature of the bimolecular interface between hormone and receptor is to use a series of specially designed photoreactive
benzophenone- ( BP- ) containing
PTH analogs in " photoaffinity scanning " of the
PTH /
PTHrP receptor . -
pubmed
In an
H2O2 - driven assay , in which
H2O2 replaced two of the
protein components ,
NADH and
O2 used in the standard assay , the proteolysed
hydroxylase retained full activity for
ethane ,
propane and
propene , but had a 2 - 3 - fold increase with
methane as substrate . -
pubmed
Using
polyoxyethylene bis ( imidazolyl carbonyl ) ( M(r ) 3350 ) as the crosslinking agent , increased thermostability of the
hydroxylase was observed . -
pubmed
Activated
methoxypolyethylene glycol ( M(r ) 5000 ) was used to modify the
hydroxylase which was now soluble in organic solvents as well as water and could be activated by
H2O2 . -
pubmed
The
glycol - modified
hydroxylase functioned well in organic solvents in the catalysis of
propene oxidation . -
pubmed
The non - reducing ends of this unit are the sites of
mycolic acid attachment and , as they also appear in
lipoarabinomannan (
LAM ) , the point of
mannose capping in some mycobacteria . -
pubmed
Upon application of degenerated
oligonucleotides and the PCR - technology a number of putative
odorant receptors have been cloned and sequenced . -
pubmed
The addition of
KCl ( from 0.05 M to 0.5 M ) to an acidic solution of monomeric
creatine kinase ( pH 1.6 ) resulted in a highly cooperative transition from the partially unfolded conformation ( UA ) to the more compact conformation ( A ) with the properties of a molten globule , as probed by
CD spectra and by fluorescence . -
pubmed
To further define the factors required for late and very late promoter expression , we first determined that the eighteen lefs were also required for expression from two other major baculovirus promoters : the late
basic 6.9 - kDa protein gene ,
p6.9 , and the very late 10 - kDa protein gene ,
p10 . -
pubmed
Within 12 - 24 h after elevation of
Ca2 + , a selective loss of the
alpha 6 beta 4 integrin complex is associated with the induction of the spinous cell marker
keratin 1 . -
pubmed
Whereas early keratinocyte maturation is linked to the selective loss of the
alpha 6 beta 4 complex , loss of both
beta 1 and
beta 4 integrin mRNA and protein occurs as cells proceed to later stages in the differentiation program as induced by 0.5 mM
Ca2 + or suspension culture . -
pubmed
Adjuvant hepatic arterial
IL-2 and
MMC , 5 - FU after curative resection of
colorectal liver metastases . -
pubmed
The regimen consisted of weekly
interleukin-2 ( 1.4 - 2x 10(6 ) units ) ,
5 - fluorouracil ( 250 mg ) by 2 - hour infusion and bolus
mitomycin C ( 4 mg ) for 6 months . -
pubmed
The efficient concentration ranges of other
vitamin D3 metabolites suggest a mediation through the
VD nuclear receptor (
VDR ) . -
pubmed
Antiinflammatory
4,5 - diarylimidazoles as selective
cyclooxygenase inhibitors . -
pubmed
Variables evaluated were : disease duration , clinical pattern , disease activity ( SLEDAI ) , cumulative damage index ( SLICC /
ACR ) , current and cumulative
prednisone dose , duration of
steroid treatment , and use of immunosuppressive agents . -
pubmed
Tanshione II - A induced a dose- and time - dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of
poly ( ADP - ribose ) polymerase in both cell lines . -
pubmed
The causative biochemical defect has been identified as a
deficiency of the enzyme fatty aldehyde dehydrogenase , a component of
fatty alcohol :
NAD + oxidoreductase . -
pubmed
Within the lumen ,
Tsp removes the
carboxy - terminal tail of the precursor of the
PSII D1 protein . -
pubmed
The
ATP - dependent metalloprotease
FtsH is bound to the thylakoid membrane , facing the stroma . -
pubmed
Since
ATP - dependent bacterial
proteases and their mitochondrial homologues are also involved in the regulation of gene expression , via their determining the levels of key regulatory proteins ,
chloroplast proteases are expected to play a similar role . -
pubmed
Interpreting trends in the binding of
cyclic ureas to
HIV-1 protease . -
pubmed
Finally we verified that the ex vivo
sst2 gene expression in
tumor samples was positively related ( P & lt ; 0.01 ) to the in vivo semiquantitative determination of
sst2 protein , assessed by
111In - pentetreotide imaging . -
pubmed
We have recently shown that
IL-3R occupancy activates a
phosphatidylcholine - specific phospholipase C , and the sustained
diacylglycerol accumulation subsequently activates
protein kinase C (
PKC ) . -
pubmed
The report of a
PKC activatable
cAMP response element ( CRE ) in the bcl-2 promoter and a role for
PKC in
bcl-2 expression in B cells led us to the hypothesis that
PKC phosphorylation activates
transcription factor CREB after
IL-3R engagement . -
pubmed
IL-4 , which is biologically active in these cells but does not use the beta common subunit , did not phosphorylate
CREB on
Ser(133 ) . -
pubmed
OBJECTIVES : This study clarified the exposure - response relationships for the organic acid
anhydrides (
OAA )
hexahydrophthalic (
HHPA ) and
methylhexahydrophthalic (
MHHPA )
anhydrides and the development of specific
immunoglobulin ( IG ) E and G antibodies and work - related symptoms . -
pubmed
CONCLUSIONS : In spite of the very low
OAA levels in the air and metabolites in the urine , there were high and exposure - related risks of specific
IgE and
IgG sensitization and of work - related symptoms for the eyes , nose ( especially
bleeding ) , and lower airways . -
pubmed
Many neurones co - expressed the
leptin and
cholecystokinin type A receptors , or
leptin receptor and
cocaine- and
amphetamine - related transcript . -
pubmed
We conclude that vagal afferent neurones that express the
cholecystokinin type A receptor and
cocaine- and
amphetamine - related transcript , may also express the long form of the
leptin receptor providing a neurochemical basis for observations of interactions between
cholecystokinin and
leptin . -
pubmed
Administration of
Y-27632 moderately suppressed the atrioventricular
nodal conduction , slightly but significantly accelerated the repolarization process , and potently increased the coronary blood flow , whereas it hardly affected the intraventricular conduction . -
pubmed
The estimated concentrations of
Y-27632 causing the currently observed effects were enough to inhibit
Rho - kinase . -
pubmed
Activation of
protein kinase C (
PKC ) by perfusion of hearts with
phorbol-12 - myristate-13 - acetate (
PMA ) or
endothelin-1 (
ET-1 ) inhibited the maximum
ATPase rate by up to 25 % and increased the
Ca2 + sensitivity of
ATPase activity and of isometric tension by up to 0.15 pCa units . -
pubmed
For comparison , the beta - adrenergic agonist
isoproterenol caused a 38 % increase in maximum
ATPase rate and a 0.12 pCa unit decrease in myofilament
Ca2 + sensitivity . -
pubmed
A primary role of
PKC phosphorylation of
cTnI may be to reduce the requirements of the contractile apparatus for both
Ca2 + and
ATP , thereby promoting efficient
ATP utilisation during contraction . -
pubmed
Low levels of
apolipoprotein A1 (
ApoA1 ) and
cholesterol in the serum and undetectable levels of
high density lipoprotein (
HDL ) and
low density lipoprotein (
LDL )
cholesterol in the blood confirmed the diagnosis of
TD . -
pubmed
Here we report that the receptor
tyrosine kinases
Ror1 and
Ror2 modulate the growth of neurites as well as their branching pattern in hippocampal neurons . -
pubmed
Upon
Ror1 or
Ror2 suppression using antisense
oligonucleotides or RNA interference ( RNAi ) , neurons extended shorter and less branched minor processes when compared to those in control cells . -
pubmed
Bcl-2 enhances
Ca ( 2 + ) signaling to support the intrinsic regenerative capacity of CNS axons . -
pubmed
Here , we report that
Bcl-2 supports axonal growth by enhancing intracellular
Ca ( 2 + ) signaling and activating
cAMP response element binding protein (
CREB ) and
extracellular - regulated kinase (
Erk ) , which stimulate the regenerative response and neuritogenesis . -
pubmed
Expression of
Bcl-2 decreases endoplasmic reticulum (
ER )
Ca ( 2 + ) uptake and storage , and thereby leads to a larger intracellular
Ca ( 2 + ) response induced by
Ca ( 2 + ) influx or axotomy in
Bcl-2 - expressing neurons than in control neurons . -
pubmed
Bcl - x ( L ) , an antiapoptotic member of the
Bcl-2 family that does not affect
ER Ca ( 2 + ) uptake , supports neuronal survival but can not activate
CREB and
Erk or promote axon regeneration . -
pubmed
These results suggest a novel role for
ER Ca ( 2 + ) in the regulation of neuronal response to
injury and define a dedicated signaling event through which
Bcl-2 supports CNS regeneration . -
pubmed
The
AtlA protein predicted to be encoded by
atlA is composed of 979
amino acids with a molecular weight of 107,279 and has a conserved
beta-1,4 - N - acetylmuramidase (
lysozyme ) domain in the C - terminal portion . -
pubmed
Sodium dodecyl sulfate extracts of
strain Xc showed two major bacteriolytic bands with molecular masses of 107 and 79 kDa , both of which were absent from a mutant with inactivated
atlA. -
pubmed
Because
ADX reduces the orexigenic actions of
NPY and
AgRP , we hypothesised that
ADX would also reduce the orexigenic action of
ghrelin . -
pubmed
Chronic
alcohol exposure resulted in significant
NTS gene regulation related to the general processes of synaptic transmission , intracellular signaling , and cation transport as well as specific neuronal functions including plasticity and
seizure behavior that could be related to
alcohol withdrawal symptoms . -
pubmed
The differentially expressed genes were also significantly enriched for enzymes of lipid metabolism ,
glucose metabolism , oxidative phosphorylation ,
MAP kinase signaling , and
calcium signaling pathways from KEGG . -
pubmed
Intriguingly , many of the genes we found to be differentially expressed in the
NTS are known to be involved in
alcohol - induced oxidative stress and/or cell
death . -
pubmed
The study provides evidence of very extensive alterations of physiological gene expression in the
NTS in the adapted state to chronic
alcohol exposure . -
pubmed
Polyamine oxidase and
acrolein as novel biochemical markers for diagnosis of
cerebral stroke . -
pubmed
BACKGROUND AND PURPOSE : We found previously that plasma levels of
acrolein ( CH2 =
CHCHO ) and
spermine oxidase (
SMO ) were well correlated with the degree of severity of
chronic renal failure . -
pubmed
The size of
stroke was nearly parallel with the multiplied value of
acrolein and total
polyamine oxidase (
AcPAO plus
SMO ) . -
pubmed
However , the
caspase inhibitors did not affect the DeltaPsim , but
cyclosporin A inhibited the collapse of DeltaPsim followed by a reduction of cell
death . -
pubmed
Lidocaine - induced apoptosis was mitochondria- and
caspase - dependent , but the collapse of DeltaPsim was independent of
caspase activation . -
pubmed
Pretreatment with
PRE-084 , a
sigma1 receptor agonist , resulted in significant increased viability in a dose - related manner . -
pubmed
The effects of
PRE-084 were antagonized by pretreatment with
sigma1 receptor antagonists (
NE-100 and
BD1047 ) , but interestingly were synergized by cotreatment with
BD1047 that also presented an affinity for the
sigma2 receptor . -
pubmed
EFFECT OF
2,4 - DICHLOROPHENOXYACETIC ACID ON PROTEOLYTIC ACTIVITY OF
RED KIDNEY BEAN PLANTS . -
pubmed
After separating tRNAs on a two - dimensional polyacrylamide gel , the positions of seven selected tRNAs were identified by Northern hybridization and their relative affinities to immobilized
exportin - t x ran x GppNHp complex were estimated in the order of tRNA(Leu)(CAG ) & gt ; tRNA(
Ser)(GCU ) , tRNA(
Leu)(CAA ) , tRNA(
Ser)(UGA ) & gt ; tRNA(
Ser)(AGA ) , tRNA(
Leu)(AAG ) & gt ; tRNA(
Arg)(ACG ) . -
pubmed
In multivariable analyses and adjusting for established risk factors and additionally for
glomerular filtration rate and for
hemoglobin ,
serum albumin ,
proteinuria , and
C - reactive protein levels , a higher level of serum
phosphorus was associated with an increased
CVD risk in a continuous fashion ( adjusted hazard ratio per increment of milligrams per deciliter , 1.31 ; 95 % confidence interval , 1.05 - 1.63 ; P=.02 ; P value for trend across quartiles = .004 ) . -
pubmed
A phase
II placebo - controlled trial of neoadjuvant
anastrozole alone or with
gefitinib in early
breast cancer . -
pubmed
We have therefore investigated whether neoadjuvant
gefitinib , an
EGFR inhibitor , might overcome biologic and clinical resistance to neoadjuvant
anastrozole in a phase
II placebo - controlled trial . -
pubmed
ORs in the combination and
anastrozole alone groups were 48 % and 61 % ( estimated difference = -13.1 % ; 95 % CI , -27.3 % to 1.2 % ) , respectively , with a nonsignificant trend against the combination ( P = .08 ) and 48 % versus 72 % ( estimated difference = -24.1 % ; 95 % CI , -45.3 % to -2.9 % ) in the
progesterone - receptor - positive subgroup , which was significant ( P = .03 ) and consistent with
Ki67 changes . -
pubmed
Some proteins first enter the endocytic pathway before appearing at the cell surface ; this is detected by treating the cells at 4 degrees and 37 degrees C. Analysis of the number of
sialic acids on proteins of cells treated at 4 degrees C identifies proteins resident at the cell surface , while cells treated at 37 degrees C internalize the
sialidase , which can then act with proteins in the endocytic compartments . -
pubmed
As shown by both methods ,
APE1 binds with the highest efficiency to DNA substrate containing 5 -sugar
phosphate group in the nick / gap , whereas
DNA polymerase beta binds to DNA duplex with a
mononucleotide gap flanked by the 5 -p group . -
pubmed
When
APE1 and
DNA polymerase beta are both present , a ternary complex
APE1 -
DNA polymerase beta - DNA is formed with the highest efficiency with DNA product of
APE1 endonuclease activity and with DNA containing 5 -flap or
mononucleotide - gapped DNA with 5 -p group . -
pubmed
Kinetic
deuterium isotope effect analysis and Hammett analysis using a series of
phenol derivatives under anaerobic conditions ( single - turnover reaction ) have indicated that the monooxygenation reaction of
phenols to
catechols by the
peroxo species of
oxyhemocyanin proceeds via electrophilic aromatic substitution mechanism as in the case of
tyrosinase . -
pubmed
The
HLA - B*3713 allele differs from the closest matching allele B*370101 by one nucleotide substitutions in exon 3 at nt 527(T-->A ) , resulting in an
amino acid change from
Val (
GTG ) to
Glu ( GAG ) at codon 152 . -
pubmed
Intranasal administration of
ovalbumin (
OVA ) formulated in an archaeal lipid mucosal vaccine adjuvant and delivery ( AMVAD ) system prepared by the addition of
CaCl2 to small unilamellar archaeosomes ( liposomes made from archaeal polar lipids ) containing encapsulated OVA , was recently shown to elicit strong and sustained OVA - specific mucosal and systemic immune responses . -
pubmed
The
D - lactate dehydrogenase is a zinc - flavoprotein with high acceptor specificity for
cytochrome c , that catalyzes the oxidation of
D - lactate into
pyruvate . -
pubmed
New oxidant used for the
post - column derivatization determination of
Malachite Green and
Leucomalachite Green residues in cultured aquatic products by high - performance liquid chromatography . -
pubmed
When the eluate was combined with 3.0 x 10(-4 ) mol L(-1 )
iodine solution , LMG was converted to MG and detected at 618 nm after
post - column derivatization . -
pubmed
Seven peptides ,
Val - Asp - Phe ( IC(50 ): 6.59 microM ) ,
Leu - Pro - Phe ( 10.59 microM ) ,
Met - Pro - Phe ( 17.98 microM ) ,
Tyr - Thr - Ala - Gly - Val ( 23.38 microM ) ,
Glu - Arg - Tyr - Pro - Ile ( 8.76 microM ) ,
Ile - Pro - Phe ( 8.78 microM ) , and
Thr - Thr - Ile ( 24.94 microM ) , were identified by liquid chromatography - mass spectrometry ( LC -
MS /
MS ) , and their IC(50 ) values were predicted by using our previously reported structure and activity models . -
pubmed
The fruit samples gained from the field trials were quantitatively analysed by gas chromatography with a
nitrogen phosphorus detector (
GC /
NPD ) for
tebuconazole residues . -
pubmed
RESULTS :
Tebuconazole residues in the fruits gained from the plot treated with the WP formulation , 14 days after application , were in the range 0.01 - 0.07 mg kg(-1 ) , while corresponding residues in the plot treated with the
WG formulation were in the range 0.01 - 0.06 mg kg(-1 ) . -
pubmed
CONCLUSION : No significant differences in the residue levels of
tebuconazole could be observed between the trials conducted with the WP and the
WG formulation . -
pubmed
Specific salivary components were
lactoferrin , secretory
IgA ,
albumin ,
lysozyme ,
mucin ,
cystatin , K+ ,
Ca2 + ,
Na + and
Cl- . -
pubmed
RESULTS : Significant associations were found between
caries , age and specific individual submandibular / sublingual salivary proteins (
lactoferrin ,
albumin ,
lysozyme ,
mucin and
cystatin ) and specific inorganic constituents ( K+ ,
Ca2 + ,
Na+ and
Cl- ) . -
pubmed
Steroid - resistant acute rejection (
SR -
AR ) and ductopenic rejection (
DR ) after liver transplantation are infrequent , but difficult to manage . -
pubmed
We conclude that a dual therapy regimen of
tacrolimus and
sirolimus can achieve a high response rate as a rescue therapy for
SR -
AR and DR , provided it is begun as soon as possible . -
pubmed
Vasopressin plays an important role in the control of blood osmolarity and vascular tone , but is also involved in many other physiological events , which are mediated mainly via three types of
vasopressin receptor :
V1R ,
V2R , and
V3R. -
pubmed
V1R primarily mediate the vascular , and
V2R the aquaretic , effects of
vasopressin . -
pubmed
Vasopressin may also interact with other receptors , like adrenergic and
angiotensin - II receptors , or with distinct biological pathways , including those of
nitric oxide and the K(
ATP ) channel . -
pubmed
Currently ,
vasopressin and
terlipressin are most commonly used to stimulate
V1R in vasodilatory
shock and
cardiac arrest , while
desmopressin , a synthetic analogue of
vasopressin , acts on
V2R ; but new molecules are becoming available in the treatment of inappropriate
antidiuretic hormone (
ADH ) secretion . -
pubmed
On the
catecholamine control of
growth hormone regulation . -
pubmed
INTERPRETATION & amp ; CONCLUSION : Our study showed high ESBL occurrence with
CTX - M as the emerging type in our hospital and
CTX - M-3 being reported for the fi rst time in India . -
pubmed
Gallic acid induces neuronal cell
death through activation of
c - Jun N - terminal kinase and downregulation of
Bcl-2 . -
pubmed
Gallic acid also induced cleavage of
poly ( ADP - ribose ) polymerase , which is strongly related to apoptosis in neurons . -
pubmed
The objectives of this investigation were to assess the ability of gastrointestinal simulation software to predict the oral absorption of the
BCS class I drugs
propranolol and
metoprolol and the
BCS class III drugs
cimetidine ,
atenolol , and
amoxicillin , and to perform in silico bioequivalence studies to assess the feasibility of extending biowaivers to
BCS class III drugs . -
pubmed
For
BCS class III drugs with intestinal absorption patterns similar to those of
cimetidine ,
atenolol or
amoxicillin , the dissolution criteria for extension of biowaivers to
BCS class III drugs warrants further investigation . -
pubmed
The substrate cleavage pattern monitored by capillary zone electrophoresis showed that
TpAraF is a classical exo - acting enzyme producing
arabinose as its end - product . -
pubmed
Synthesis and pharmacological evaluation of
fluorine - containing
D3 dopamine receptor ligands . -
pubmed
These compounds were also evaluated for intrinsic activity at
D ( 2 ) and D ( 3 ) receptors using a
forskolin - dependent
adenylyl cyclase assay . -
pubmed
Furthermore , compound 20e , which has the highest binding affinity and selectivity for the
D ( 3 ) receptor ( K(i ) = 0.17 nM for D(3 ) , 163 - fold selectivity for
D ( 3 ) vs D ( 2 ) receptors ) , represents a candidate
fluorine-18 radiotracer for in vivo PET imaging studies on the regulation of
D ( 3 ) receptor expression . -
pubmed
Incorporation of interactions between two
BCAs did not greatly affect the disease dynamics except when a mycoparasitic and , to a lesser extent , an antibiotic - producing
BCA was involved . -
pubmed
OBJECTIVES :
Beryllium sensitisation ( BeS ) and
chronic beryllium disease (
CBD ) are caused by exposure to
beryllium with susceptibility affected by at least one well - studied genetic host factor , a
glutamic acid residue at position 69 ( E69 ) of the
HLA - DPbeta chain (
DPbetaE69 ) . -
pubmed
To further unmask the mechanics governing extracellular
dopamine levels we used intravenous injection of the
vesicular monoamine transporter (
VMAT2 ) inhibitor ,
tetrabenazine , to deplete
dopamine storage and increase cytoplasmic
dopamine in the nerve terminals . -
pubmed
Tetrabenazine almost abolished phasic
dopamine release but increased extracellular
dopamine to ~500 nM , presumably by inducing reverse transport by
dopamine transporter (
DAT ) . -
pubmed
Along the same way shown by
imatinib in
chronic myeloid leukemia , compounds that selectively target
ALK are bringing a revolution in the treatment of
ALK - positive tumors . -
pubmed
Crizotinib has just been approved , and new more potent
ALK inhibitors will shortly follow . -
pubmed
Total
cholesterol (
TC ) and
triglyceride (
TG ) of plasma were tested using Toshiba 120 Automatic Biochemical Analyzer . -
pubmed
There has been a longstanding interest in non - steroidal anti inflammatories in
FAP where trials have shown regression of
polyps with the " pro drug"
sulindac and the selective
COX2 inhibitors though impact on long - term
cancer risk is not confirmed . -
pubmed
For those who took
aspirin ( or AP ) for a minimum of 2 years ( per protocol ) the HR was 0.41 ( CI 0.19 - 0.86 p = 0.02 ) and the
IRR , 0.37 ( CI 0.18 - 0.78 p = 0.008 ) . -
pubmed
tuberculosis - DNA gyrase apoprotein structures and
topoII -
DNA-6FQ complexes were used for defining the
6 - fluoroquinolones ( 6 - FQs ) binding pockets . -
pubmed
We show that
nonribosomal peptide synthetases ClbN and
ClbB assemble and process an
N - acyl - D - asparagine prodrug scaffold that serves as a substrate for the periplasmic D - amino peptidase
ClbP. -
pubmed
Lectin PHA - L Conjugates ( PHA - L , Invitrogen L - 11270 , ) was injected into the MVN as an anterograde tracer , and 5 % FluoSpheres
carboxylate - modified microspheres ( MFS , Molecular Probe F-8793 ) was injected into the contralateral peripheral vestibule using as a retrograde tracer . -
pubmed
We describe five genes ,
uricase ,
5 - hydroxyisourate hydrolase ,
parahox neighbor B ,
allantoinase , and
allantoicase , each coding for one of the five enzymes involved in
urate degradation to
urea , and report the identification of a putative second form of
5 - hydroxyisourate hydrolase that is characteristic of the genus Latimeria . -
pubmed
Changes in
systolic blood pressure (
SBP ) and diastolic blood pressure (
DBP ) were examined by randomization arm ( intensive lifestyle change [ ILS ] ,
metformin 850 mg twice daily , or placebo ) . -
pubmed
Associations between changes in blood pressure and changes in
sex hormone - binding globulin ,
estradiol ,
testosterone , and
dehydroepiandrosterone were also examined . -
pubmed
Estrogen users and nonusers randomized to ILS had similar decreases in
SBP ( -3.3 vs -4.7 mm Hg , P = 0.45 ) and
DBP ( -3.1 vs -4.7 mm Hg , P = 0.16 ) . -
pubmed
Also ,
alcohol use in women decreases the success of
infertility treatment , such as in vitro fertilization (
IVF ) . -
pubmed
Our goal was to determine if there were differences in
IVF outcomes and
alcohol use parameters among at - risk drinkers randomized to a brief intervention ( BI ) versus assessment only ( AO ) . -
pubmed
CONCLUSIONS : BI and AO contributed to a decrease in
alcohol use and did not demonstrate differences in
IVF outcomes . -
pubmed
Antitumor activity of the selective pan -
RAF inhibitor
TAK-632 in
BRAF inhibitor - resistant
melanoma . -
pubmed
Recently , the
BRAF inhibitors
vemurafenib and
dabrafenib have emerged as promising agents for the treatment of
melanoma patients with
BRAF - activating mutations . -
pubmed
Here , we demonstrate that the selective pan -
RAF inhibitor
TAK-632 suppresses
RAF activity in
BRAF wild - type cells with minimal
RAF paradoxical activation . -
pubmed
Our analysis using RNAi and
TAK-632 in preclinical models reveals that the
MAPK pathway of
NRAS - mutated
melanoma cells is highly dependent on
RAF . -
pubmed
We also show that
TAK-632 induces
RAF dimerization but inhibits the kinase activity of the
RAF dimer , probably because of its slow dissociation from
RAF . -
pubmed
As a result ,
TAK-632 demonstrates potent antiproliferative effects both on
NRAS - mutated
melanoma cells and
BRAF - mutated
melanoma cells with acquired resistance to
BRAF inhibitors through
NRAS mutation or
BRAF truncation . -
pubmed
Furthermore , we demonstrate that the combination of
TAK-632 and the
MAPK kinase (
MEK ) inhibitor
TAK-733 exhibits synergistic antiproliferative effects on these cells . -
pubmed
Our findings characterize the unique features of
TAK-632 as a pan -
RAF inhibitor and provide rationale for its further investigation in
NRAS - mutated
melanoma and a subset of
BRAF - mutated
melanomas refractory to
BRAF inhibitors . -
pubmed
Polyacrylamide - gel electrophoresis showed the existence of nine
isoenzymes of
SOD . -
pubmed
Results showed that , precipitation pH value increased from
spring to summer , soluble
Cd concentration of
spring runoff was significantly higher than that of summer rainfall runoff , which presented an obviously seasonal heterogeneity and had a negative correlation with rainfall pH value , and rainfall pH value can obviously impact soil soluble
Cd transportation into surface runoff charge . -
pubmed
Skeletal muscle is an important target tissue for
adiponectin where it regulates
glucose and
fatty acid metabolism directly and via
insulin sensitizing effects . -
pubmed
On the treated side ,
CBF and CBV values were higher before and 7 days after cranioplasty than at 3 months after surgery , whereas
MTT values were lower at 7 days than at 3 months after surgical treatment . -
pubmed
In this study , we demonstrate that deletion of the
RR - encoding gene ,
abrC3 (
SCO4596 ) , results in a dramatic decrease in
actinorhodin (
ACT ) and
undecylprodiginine ( RED ) production and delays morphological development . -
pubmed
Characterization of the mode of action of
isohymenialdisine revealed that it directly acts on
PKR by inhibiting autophosphorylation , perturbs the
PKR -
eIF2alpha phosphorylation axis , and can be modeled into the
PKR ATP binding site . -
pubmed
Conversely ,
amino acid exchanges in the two GARRPR motifs of
Bag-1L can impair the interaction between
Bag-1L and
AR without altering the ability of
Bag-1L to bind to chromatin . -
pubmed
Furthermore , the mutant
Bag-1L increases
androgen - dependent activation of a subset of
AR targets in a genome - wide transcriptome analysis , demonstrating a repressive function of the
GARRPR /
BF-3 interaction . -
pubmed
However ,
RAGE deletion did not completely prevent
inflammation or
vascular damage , possibly because the induction of mitochondrial oxidative stress by
dicarbonyls also contributes to
inflammation and
atherogenesis . -
pubmed
Extrasynaptic
glutamate release through
cystine / glutamate antiporter contributes to
ischemic damage . -
pubmed
During
brain ischemia , an excessive release of
glutamate triggers neuronal
death through the overactivation of
NMDA receptors ( NMDARs ) ; however , the underlying pathways that alter
glutamate homeostasis and whether synaptic or extrasynaptic sites are responsible for excess
glutamate remain controversial . -
pubmed
Blockade of excitatory
amino acid transporters or vesicular
glutamate release did not inhibit
ischemia - gated currents or
neuronal damage after
OGD . -
pubmed
Furthermore ,
glutamate released by the
cystine /
glutamate antiporter activated extrasynaptic , but not synaptic , NMDARs , and blockade of extrasynaptic NMDARs reduced
ischemia - gated currents and cell damage after
OGD . -
pubmed
The
aryl hydrocarbon receptor : a review of its role in the physiology and pathology of the integument and its relationship to the
tryptophan metabolism . -
pubmed
The
aryl hydrocarbon receptor (
AHR ) is a cytosolic receptor for low molecular weight molecules , of which the most widely recognized ligand is
2,3,7,8 - tetrachlorodibenzo - p - dioxin (
TCDD ) , and the most widely recognized effect ,
chloracne . -
pubmed
I provide a review outlining the role
AHR plays in the development , cellular oxidation / antioxidation , responses to ultraviolet light , melanogenesis , epidermal barrier function , and immune regulation and its relationship to
tryptophan metabolism . -
pubmed
MBT also decreased
triacylglycerol and total
cholesterol levels in the muscle by 30 % , which was correlated with suppression of lipogenic genes such as
ACC ,
C / EBP alpha ,
PGC-1 alpha , and
PPAR gamma . -
pubmed
In particular , decreased levels of
p - ERK1 / 2 ,
PPAR gamma , and
C / EBP alpha in the
MBT - treated groups suggest that
MBT might inhibit adipogenesis and decrease differentiation via the
MEK /
ERK pathway . -
pubmed
Fasting
glucose before breakfast on workdays ( after 7.1 + - 0.8 h ) was not significantly different between the three interventions (
CS 4.1 + - 0.5 mmol / l ,
MCS 4.6 + - 0.7 mmol / l , pasta 4.3 + - 0.9 mmol / l ) . -
pubmed
In this sense , we construct three indices including Net Global Warming Potential ( NGWP ) and
Nitrogen Surplus (
NS ) , simulated by a biogeochemistry model , and also an Environmental Impact Quotient ( EQI ) for paddy fields . -
pubmed
Moreover ,
rutin decreased macrophage infiltration , proinflammatory
cytokine expression and phosphorylation of
nuclear factor - kappaB p65 . -
pubmed
Furthermore ,
rutin inhibited extracellular matrix accumulation by reducing expression of
type I / III collagen and
fibronectin . -
pubmed
Rutin also prevented the epithelial - mesenchymal transition processes of renal tubular cells by decreasing
alpha - smooth muscle actin expression and retaining
E - cadherin expression . -
pubmed
These effects of
rutin were in parallel with the reductions in
Smad3 activity and pivotal to the fibrogenic potential of
TGF - beta1 . -
pubmed
Taken together , the renoprotective effects of
rutin in
obstructive nephropathy were likely due to anti - inflammatory effects and inhibition of
TGF - beta1 /
Smad3 signaling . -
pubmed
Secondary outcome measures were
tumour size ,
nodal positivity and radioactive
iodine ablation rate . -
pubmed
A reduced
graphene oxide - based fluorescence resonance energy transfer sensor for highly sensitive detection of
matrix metalloproteinase 2 . -
pubmed
A novel fluorescence nanoprobe ( reduced nano -
graphene oxide [
nrGO]/
fluorescein isothiocyanate - labeled peptide [ Pep -
FITC ] ) for ultrasensitive detection of
matrix metalloproteinase 2 (
MMP2 ) has been developed by engineering the Pep -
FITC comprising the specific
MMP2 substrate domain ( PLGVR ) onto the surface of nrGO particles through non - covalent linkage . -
pubmed
Upon cleavage of the
amide bond between
Leu and
Gly in the Pep -
FITC by
protease -
MMP2 , the
FITC bound to nrGO was separated from nrGO surface , disrupting the fluorescence resonance energy transfer process and resulting in fluorescence recovery of
FITC . -
pubmed
Under optimal conditions , the fluorescence recovery of nrGO / Pep -
FITC was found to be directly proportional to the concentration of
MMP2 within 0.02 - 0.1 nM. -
pubmed
The X - ray crystallographic analysis confirms that while
CIP and
CIT manifest planar structures ,
DPT shows a curved pi - conjugated
carbon skeleton . -
pubmed
In this study , we found that
HSP90 was cleaved to a 55kDa protein after treatment with proteasome inhibitors including
MG132 in
leukemia cells but was not cleaved in other tissue - derived cells . -
pubmed
HSP90 has two major isoforms (
HSP90alpha and
HSP90beta ) , and both were cleaved by
MG132 treatment . -
pubmed
MG132 treatment also induced a decrease in
HSP90 client proteins . -
pubmed
MG132 treatment generated ROS , and the cleavage of
HSP90 was blocked by a ROS scavenger ,
N - acetylcysteine (
NAC ) . -
pubmed
MG132 activated several
caspases , and the activation was reduced by pretreatment with
NAC . -
pubmed
Based on an inhibitor study , the cleavage of
HSP90 induced by
MG132 was dependent on
caspase 10 activation . -
pubmed
MG132 upregulated
VDUP-1 expression and reduced the
GSH levels implying that the regulation of redox - related proteins is involved . -
pubmed
Taken all together , our results suggest that the cleavage of
HSP90 by
MG132 treatment is mediated by ROS generation and
caspase 10 activation . -
pubmed
Our results indicated that
PcTx1can mainly regulate
ASIC1a gating process through
hydrogen bonds , which can affect their relative positions of several key domains in
ASIC1a , further , a long - range conformational changes path was determined , which is composed of beta1 ,
beta2 , beta10 , alpha6 , alpha7 , beta11 , and beta12 in
ASIC1a . -
pubmed
It is reported that
tert - butylhydroquinone (
tBHQ ) , a
nuclear factor erythroid 2 - related factor 2(
Nrf2)-inducer , has a variety of pharmacological activities such as anti - oxidation and anti - inflammatory effect . -
pubmed
It increased PVN level of reactive
oxygen species ,
gp91phox ,
IL-1beta ,
IL-6 , p -
IKKbeta and
nuclear factor - kappa B (
NF - kappaB ) activity , decreased PVN level of
Nrf2 and
Cu / Zn - SOD . -
pubmed
In this paper , the prediction success of DDIs involving various
cytochrome P450 isoenzyme (
CYP ) modulators namely
ketoconazole ( a competitive inhibitor of
CYP3A ) ,
itraconazole ( a competitive inhibitor of
CYP3A ) ,
clarithromycin ( a mechanism - based inhibitor of
CYP3A ) ,
quinidine ( a competitive inhibitor of
CYP2D6 ) ,
paroxetine ( a mechanism - based inhibitor of
CYP2D6 ) ,
ciprofloxacin ( a competitive inhibitor of
CYP1A2 ) ,
fluconazole ( a competitive inhibitor of
CYP2C9/
2C19 ) and
rifampicin ( an inducer of
CYP3A ) were assessed using Simcyp software . -
pubmed
Yet , it ranges from &
lt;1 % to nearly 100 % compared to AUG translation , depending on surrounding sequences , including Kozak , and possibly additional
nucleotide contexts . -
pubmed
5MP represses non - AUG translation by competing with
eIF5 for the
Met - tRNAi - binding factor
eIF2 . -
pubmed
Consistently ,
eIF5 increases , whereas
5MP decreases translation of
NAT1 /
EIF4G2 /
DAP5 , whose sole start codon is GUG . -
pubmed
Furthermore , GNF - SERS combined with quantum chemical calculation identified
cysteine - derived
glutathione and
hypotaurine (
HT ) as tumour - dominant and parenchyma - dominant metabolites , respectively . -
pubmed
CD44 knockdown in
cancer diminished
glutathione , but not
HT in
tumours . -
pubmed
Mechanisms whereby
tumours sustained
HT under
CD44 - knockdown conditions include upregulation of
PHGDH ,
PSAT1 and
PSPH that drove glycolysis - dependent activation of
serine /
glycine - cleavage systems to provide one -
methyl group for
HT synthesis . -
pubmed
HT was rapidly converted into
taurine in
cancer cells , suggesting that
HT is a robust anti - oxidant for their survival under
glutathione - suppressed conditions . -
pubmed
After 7 , 14 , and 30 days
post - surgery , a fragment of each specimen was fixed in - 80 C liquid
nitrogen for zymographic evaluation , while the remaining was fixed in 10 %
formalin for histological birefringence analysis . -
pubmed
We report here the three - dimensional structure of
McpU - LBD in complex with
putrescine at a resolution of 2.4 A , which fitted well a solution structure generated by small - angle X - ray scattering . -
pubmed
Putrescine bound to a negatively charged pocket in the membrane distal module of
McpU - LBD . -
pubmed
Synthesis , in vitro antiproliferative activity , and
kinase inhibitory effects of
pyrazole - containing
diarylureas and
diarylamides . -
pubmed
The crystalline phases of these materials have PE chromophores that are twisted due to non - covalent
perfluoroarene - arene ( ArF - ArH ) interactions involving
perfluorophenyl pendants and the terminal rings of the
PE chromophore , resulting in interrupted conjugation and an absence of chromophore aggregation . -
pubmed
Here , we present a surface - enhanced Raman scattering ( SERS ) platform made by large - scale self - assembly of
Au nanoparticle (
NP ) arrays at the
cyclohexane / water interface and its use for pesticides residues trace detection . -
pubmed
Transmission electron microscopy (
TEM ) images demonstrate a good uniformity of interparticle distances ( 2 - 3 nm ) in the
Au NP arrays . -
pubmed
However , upon 1 h of ultrasonic processing at 80 C , a significant increase of oxidation was observed with high
peroxide and
p - anisidine values in the
post - processed oils ; meanwhile , the amount of
unsaturated fatty acids decreased as well . -
pubmed
Photolysis separates the
taxane from the
B12 cytoplasmic anchor , enabling the drug to exit the RBC carrier . -
pubmed
A covalently appended
Cy5 antenna sensitizes the conjugate (
Cy5 - B12 - TAX ) to far red light , thereby circumventing the intense light absorbing properties of
hemoglobin ( 350 - 600 nm ) . -
pubmed
Microscopy and imaging flow cytometry reveal that
Cy5 -
B12 -
TAX - loaded mRBCs act as drug carriers . -
pubmed
The isolate showed
hydrocarbon degrading
enzyme activities namely
alkane hydroxylase ,
catechol 1,2 - dioxygenase and
catechol 2,3 - dioxygenase . -
pubmed
The level of
C23O activity was 9.63 times higher than
C12O thus suggesting active involvement of meta - cleavage pathway . -
pubmed
Importantly ,
USP7 small - molecule inhibitors ,
P5091 and
P22077 , inhibit
SOCS1 expression and enhance
IFN - I antiviral efficacy . -
pubmed
We have evaluated the
toxicity of
flutianil , which was developed by
OAT Agrio . -
pubmed
These include promotion of mucosal antibody and anti - microbial peptide production , coupled with a strong suppression of inflammatory
cytokines and reactive
oxygen species . -
pubmed
Pyruvate kinase activity and
lactate detection assays were performed to analyze the effects of
Sam68 on aerobic glycolysis . -
pubmed
Sam68 significantly promoted cell proliferation and caused a decrease of
PKM1 /
PKM2 ratio , resulting in the metabolism of
glucose switched from oxidative phosphorylation to glycolysis in
CRC cells . -
pubmed
Besides ,
Sam68 enhanced
PKM2 mRNA transport from the nucleus to cytoplasm and increased the expression of
PKM2 protein , resulting in elevated
pyruvate kinase activity and
lactate production . -
pubmed
sanctori ) , harvested from the Algerian coast , we aimed to study their proximate composition ,
fatty acid profile and
angiotensin - converting enzyme (
ACE ) inhibitory activity . -
pubmed
The four holothurian species showed a high amount of
protein ( 49.26 - 69.34 % ) , and an impressive lipid profile of 27
fatty acids , mainly composed of polar
fatty acids ( 91.16 - 93.85 % ) , with a high
polyunsaturated fatty acids (
PUFA ) content ( 50.90 - 71.80 % ) , particularly
eicosapentaenoic acid (
EPA ) ( 5.07 - 8.76 % ) and
docosahexaenoic acid (
DHA ) ( 4.86 - 7.25 % ) . -
pubmed
Anti -
tumor and Phenotypic Regulation Effect of
Matrine on Dendritic Cells through Regulating
TLRs Pathway . -
pubmed
And the killing effect of
DAK were measured by
MTT assay . -
pubmed
RESULTS :
Matrine significantly increased the mRNA expression of
TLR7 ,
TLR8 ,
myeloid differentiation factor 88 (
MyD88 ) ,
tumor necrosis factor receptor - associated factor 6 (
TRAF-6 ) and
I kappa B kinase (
IKK ) , as well as the protein expression of
TLR7 and
TLR8 , and up - regulated the levels of
interleukin-12 (
IL-12 ) ,
IL-6 and
tumor necrosis factor - alpha (
TNF - alpha ) , meanwhile , it also increased the expressions of
MHC - II ,
CD54 ,
CD80 and
CD86 in DCs . -
pubmed
CONCLUSION :
Matrine plays an anti -
tumor role by regulating
TLRs signal transduction pathway , promoting the secretion of
inflammatory cytokines and enhancing immune function . -
pubmed
Mineral carbonation routes have been extensively studied for almost two decades at Abo Akademi University , focusing on the extraction of
magnesium from
magnesium silicates using
ammonium sulfate (
AS ) and/or
ammonium bisulfate (
ABS ) flux salt followed by carbonation . -
pubmed
This study focused on the separation of
AS ,
ABS and aqueous
ammonia using different setups of bipolar membrane electrodialysis using both synthetic and rock - derived solutions . -
pubmed
ORX - CFW and ORX - MBW partially prevented the reduction of serum
testosterone concentrations and decreased
insulin resistance , compared to the ORX - CON . -
pubmed
Affinity labeling of
gamma - glutamyl transpeptidase and location of the
gamma - glutamyl binding site on the light subunit . -
pubmed
Gamma - Glutamyl transpeptidase , which consists of two nonidentical subunits , is rapidly inactivated with respect to its
transpeptidase and
hydrolase activities by the
gamma - glutamyl analogs
6 - diazo-5 - oxo - L - norleucine and
L - azaserine . -
pubmed
Inactivation , which is prevented by
gamma - glutamyl substrates ( but not by acceptor substrates ) , is accelerated by
maleate , which was previously shown to enhance utilization of
glutamine by
transpeptidase . -
pubmed
The findings , which indicate that these
enzymes have similar
gamma - glutamyl binding subunits , are relevant to the structure - function relationships of this membrane - bound enzyme and its physiological role . -
pubmed
The changes observed during the estrous cycle and after castration suggest that gonadal
steroids play a direct role in the control of hypothalamic Gn -
RH . -
pubmed
By virtue of the conformational rigidity of the
decalols , possible inferences concerning the stereochemical aspects of the interaction of the
ephedrine analogs with
adrenergic neurone - receptor sites are discussed . -
pubmed
Effects of
naltrindole and
nor - binaltorphimine treatment on antinociception induced by sub - acute selective
mu opioid receptor blockade . -
pubmed
Cypridina luciferin analog (
CLA ) dependent chemiluminescence was used to detect
superoxide anion radicals . -
pubmed
The
Type I ( mineralocorticoid ) receptor has identical affinities in vitro for
cortisol and
aldosterone . -
pubmed
It has been suggested that the selective role of
aldosterone in regulating
sodium homeostasis relies on the microsomal enzyme
11 beta - hydroxysteroid dehydrogenase (
11 - HSD ) . -
pubmed
Gpp ( NH ) p and
GTP gamma S inhibited
phospholipase C at nanomolar concentrations . -
pubmed
Increasing concentrations of
Gpp ( NH ) p and
GTP gamma S reversed the inhibitory effects and stimulated
phospholipase C activity . -
pubmed
Preincubation of coated vesicles with
pertussis toxin blocked the poorly - hydrolyzable
GTP - analogs ' inhibitory effects on
phospholipase C. -
pubmed
These data indicate that
guanine nucleotides exert a dual regulatory control of
phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a
pertussis toxin - sensitive
G - protein . -
pubmed
Thyroglobulin antibody and
thyroid peroxidase antibody were measured by highly sensitive assays which are based on the direct interaction between antibody and
radiolabelled antigen . -
pubmed
Our findings suggest a physiological circadian rhythm of spontaneous and LPS - induced
IL-1 production of monocytes , independent of physiological
cortisol levels , whereas unphysiological amounts of endogenous and exogenous glucocorticoids cause a substantial inhibition of
IL-1 production with a latency time of 8 hours . -
pubmed
Modulation of
cytochrome oxidase activity by inorganic and organic
phosphate . -
pubmed
The activity of
cytochrome oxidase reconstituted into phospholipid vesicles has been studied as a function of
orthophosphate ,
ATP and
inositol hexakisphosphate concentrations . -
pubmed
This effect is related to a
phosphate - dependent decrease in the rate constant for
ferrocytochrome c oxidation observed in the presence of ionophores . -
pubmed
The data can not be interpreted simply on the basis of ionic strength , which is known to limit
cytochrome c binding to
cytochrome oxidase , since
cytochrome oxidase - containing vesicles responded differently to
phosphate depending on the energization state of the
phospholipid membrane . -
pubmed
Treatment with
lovastatin and
cholestyramine can inhibit the direct synthesis pathway in this species , thereby lowering LDL
apo B concentrations . -
pubmed
The possibility that the direct synthesis pathway was confined to a specific subclass of
LDL and the effect of
lovastatin and
cholestyramine on the metabolism of
LDL subfractions were also investigated . -
pubmed
Kinetic analysis of
apo B specific activity curves following
lovastatin treatment indicated that
LDL apo B pool size was decreased by 25 % ( P less than .025 ) , which was due entirely to a 70 % ( P less than .025 ) decrease in the direct synthesis of LDL
apo B , since
VLDL - derived
apo B , and
LDL fractional catabolic rate ( FCR ) were not affected . -
pubmed
T helper cell interactions with " presenting " cells and " target " B lymphocytes are inhibited by
monoclonal antibodies directed either to the
hapten or to
I - A molecules on target cells . -
pubmed
Carbachol induces desensitization of cardiac
beta - adrenergic receptors through
muscarinic M1 receptors . -
pubmed
Incubation of enzymatically dissociated cardiac myocytes with
carbachol leads to a time- and concentration - dependent loss of
beta - receptors assayable with
[ 3H ] CGP-12177 . -
pubmed
The
carbachol effects are not influenced by
gallamine or
oxotremorine which interact with the
high - affinity ( M2 ) muscarinic receptors . -
pubmed
These results suggest that
carbachol - induced desensitization is secondary to activation of
protein kinase C by
diacylglycerols generated through
M1 receptor - linked
phosphoinositide hydrolysis . -
pubmed
Timolol inhibits
adenylate cyclase activity in the iris - ciliary body and trabecular meshwork of the eye and blocks activation of the enzyme by
salbutamol . -
pubmed
It is suggested that the
hypotensive action of
timolol on intraocular pressure results from structural and functional changes induced on the plasma membranes of the iris - ciliary body and trabecular meshwork by the
thiadiazole group of the molecule , and , also , from the occupation of the
adrenergic receptors of the iris - ciliary body by the
tert - butylamino-2 - hydroxypropoxy part of the compound . -
pubmed
The authors ' approach to the design of
DNA polymerase - specific inhibitors , an approach based on the mechanism of action of
6 -( p - hydroxyphenylazo ) uracil , has been to disguise nucleic acid bases to mimic the
purine substrates
dGTP and
dATP . -
pubmed
Specifically , the strategy has been to synthesize bases with substituents that endow them with the capacity : to seek and react with unique features of the active site of a
polymerase ; and to form H bonds with complementary template
pyrimidines apposing the active site . -
pubmed
This strategy has yielded a series of novel , enzyme - specific
dATP and
dGTP analogues which are non - polymerizable and which inhibit their target
polymerase by sequestering it to a complementary
pyrimidine residue in primer : template . -
pubmed
Principles established with the
pyrimidine derivatives have led to the development of bona fide
purine nucleotide analogues which act as
DNA polymerase inhibitors of high selectivity and unprecedented potency . -
pubmed
For example ,
BuPdGTP , the
2 '- deoxyribonucleoside 5 '- triphosphate of
N2 -( p - n - butylphenyl ) guanine (
BuPG ) , lacks discernible activity against
mammalian polymerases beta and gamma , whereas it inhibits
mammalian pol alpha with a Ki of less than 10 nanomolar . -
pubmed
Currently , the authors are exploiting
BuPdGTP ,
BuPdGDP , and similar
butylanilino derivatives of
dATP to probe the active site of
pol alpha and to develop other N2 - substituted analogues which can bind selectively to the substrate sites of other important
polymerases and
nucleotide binding proteins . -
pubmed
Effect of
aluminum on
parathyroid hormone secretion . -
pubmed
In all these fusions ,
beta - galactosidase activity was shown to be sensitive to catabolite repression by
glucose and to be inducible by polygalacturonate ,
galacturonate , and other intermediates of polygalacturonate catabolism . -
pubmed
2 - Keto-3 - deoxygluconate appeared to be the true inducer of
kduD expression resulting from
galacturonate degradation . -
pubmed
Characterization of the purple intermediate in the anaerobic reaction of
D - amino - acid oxidase with
D - alanine . -
pubmed
To measure
plasmin generation in these samples , we used the chromogenic
plasmin substrate
D - Val - Leu - Lys - p - nitroanilide , which liberates
p - nitroaniline upon cleavage . -
pubmed
The 21st chromosome has been implicated in the elevated activity of
superoxide dismutase , a finding of significance in regard to potential intracellular damage from increased levels of
peroxide . -
pubmed
Amino acid sequence of a region in
rabbit skeletal muscle glycogen synthase phosphorylated by
cyclic AMP - dependent protein kinase . -
pubmed
Purification of cardiac sarcolemmal vesicles : high
sodium pump content and
ATP - dependent ,
calmodulin - activated
calcium uptake . -
pubmed
The
Ca2 + pump was activated by
calmodulin . -
pubmed
This activation was removed by addition of
trifluoperazine , a specific inhibitor of
calmodulin . -
pubmed
When mean arterial pressure was decreased by 15 mmHg by
adrenomedullin , the increases in heart rate and renal sympathetic nerve activity were 53 + /- 8 beats / min and 78 + /- 13 % , respectively , which were significantly smaller than those induced by intravenous injection of
sodium nitroprusside ( 102 + /- 14 beats / min and 155 + /- 34 % , respectively ) . -
pubmed
More than 86.0 % were resistant to
ampicillin , and the addition of the
beta - lactamase inhibitor
sulbactam did not restore its activity . -
pubmed
Hybridomas secreting
IgG1 and
IgG2b mAb , specific for the
N - acetyl - glucosamine (
GlcNAc ) residues of streptococcal group A
carbohydrate , were previously generated from a hybridoma secreting a
mouse IgG3 mAb . -
pubmed
Nevertheless , the
IgG3 mAb binds more effectively than the V region - identical
IgG1 or
IgG2b mAb to each of three
strains of group A streptococci that display different amounts of terminal
GlcNAc residues on their cell walls . -
pubmed
The magnitude of the subclass - associated differential in binding varies with the target
strain , and , whereas the
IgG3 mAb binds best to the
strain expressing an intermediate amount of
GlcNAc , the
IgG1 and
IgG2b mAb and
IgG3 - derived F(ab')2 fragments bind best to the
strain expressing the highest amount of
GlcNAc . -
pubmed
The
IgG3 mAb also binds better than the
IgG1 and
IgG2b mAb to solid - phase
GlcNAc50 -
BSA , but the
IgG2b mAb binds best to otherwise identical conjugates with lower ratios of
GlcNAc to
BSA ( 20:1 , 10:1 , 5:1 , and 1:1 ) . -
pubmed
These results suggest that epitope density can significantly influence the magnitude of
IgG subclass - associated binding differences , and that structural differences in the CH regions , particularly the CH2 and
CH3 domains , can influence the apparent specificities of
IgG molecules for multivalent
Ag . -
pubmed
Warfarin derivatives exert both their
embryopathic and their fetopathic effects via pharmacologic action : inhibition of gamma carboxylation of
osteocalcin and a similar carboxylation of the
vitamin K dependent clotting factors . -
pubmed
Administration of
carbon tetrachloride (
CCl4 ) induced a significant decrease in liver
regucalcin concentration and a corresponding elevation of serum
regucalcin concentration at 24 h after the administration . -
pubmed
Meanwhile ,
serum transaminases (
GOT and
GPT ) activities were significantly increased by
CCl4 administration , indicating that
liver injury is induced . -
pubmed
Substance abuse in patients was an independent inverse determinant of TC value ( p = 0.05 ) ;
TG value correlated positively with
alcohol use ( p & lt ; or = 0.1 ) and
substance abuse ( p & lt ; 0.05 ) . -
pubmed
Trunk LTM (
R2 = .37 ) , total - body fat tissue mass ( [ FTM ]
R2 = .04 ) ,
androstenedione (
R2 = .03 ) , and total
triiodothyronine ( [ T3 ]
R2 = .02 ) were all significantly independently associated with REE . -
pubmed
Together they explained 46 % ( model
R2 ) of the interindividual variation in REE , with a standard error of estimate ( SEE ) of 549 kJ / d. We conclude that the interindividual variation in REE is explained mainly by differences in the visceral component of LTM , with additional minor information from the total - body FTM ,
androstenedione , and T3 . -
pubmed
cerevisiae resembled that of bacteria ( reported earlier by us ) in several aspects : first ,
sulfate - S was used in preference to that of
sulfonate , when both were present ; second , mutants unable to use
sulfate as a source of
sulfur because of deficiencies in
ATP sulfurylase ,
adenylylsulfate kinase (
APS kinase ) or
PAPS reductase were able to utilize
sulfonates ; and third , mutants deficient in
sulfite reductase were unable to utilize
sulfonates . -
pubmed
A subpopulation of cells express both
N - cadherin and
QH-1 antigen suggesting that endocardial and myocardial cells may arise from a common precursor population and that
N - cadherin regulation may be a mechanism underlying specific cell sorting of these two cell populations during heart development . -
pubmed
After
G418 selection , the control transfectant clones transfected with pH8 vector not containing
c - fos DNA insertion changed their original fibroblastic shape into dendritic cells . -
pubmed
Inhibition of
rat liver cytochrome P450 isozymes by
isothiocyanates and their conjugates : a structure - activity relationship study . -
pubmed
All
isothiocyanates inhibited
PROD more readily than
EROD . -
pubmed
Increases in the
alkyl chain length of
arylalkyl isothiocyanates to
C6 resulted in an increased inhibitory potency in these assays ; at longer
alkyl chain lengths ( C8 -
C10 ) the inhibitory potency declined . -
pubmed
The IC50s for
phenethyl isothiocyanate (
PEITC ) were 47 , 46 and 1.8 microM for
EROD ,
MROD and
PROD , respectively . -
pubmed
Substitution of an additional
phenyl group on
PEITC also increased the inhibitory potency ; the IC50s for
1,2 - diphenylethyl isothiocyanate (
1,2 - DPEITC ) and
2,2 - diphenylethyl isothiocyanate (
2,2 - DPEITC ) were 0.9 and 0.26 microM for
EROD , and 0.045 and 0.13 microM for
PROD , respectively . -
pubmed
These results suggest the potential of
isothiocyanates as structural probes for studying
P450 isozymes . -
pubmed
In addition , the inhibitory activity of
isothiocyanates for
PROD correlated with the previously demonstrated
tumor inhibitory potency in
( 4 - methylnitrosamino )- 1 -( 3 - pyridyl )- 1 - butanone (
NNK ) induced
A / J mouse lung tumor bioassays , which supports earlier findings that
P450 2B1 is one of the major
isozymes involved in
NNK activation and that inhibition of this
isozyme is an important mechanism for the chemopreventive activity of
isothiocyanates . -
pubmed
The magnitude of the dilution was in between that recorded for
albumin and for
sodium . -
pubmed
It has been suggested that the biotransformation of
oxybutynin is dependent on
CYP3A. -
pubmed
Because
itraconazole , a widely used
mycotic , is a potent inhibitor of
CYP3A4 , we wanted to study a possible interaction between
oxybutynin and
itraconazole . -
pubmed
CONCLUSIONS :
Itraconazole moderately increases serum concentrations of
oxybutynin , probably by inhibiting the
CYP3A - mediated metabolism . -
pubmed
Since about 90 % of the antimuscarinic activity of
oxybutynin is attributable to
N - desethyloxybutynin , any interaction of
oxybutynin with
CYP3A4 inhibiting drugs has only minor clinical significance . -
pubmed
Recombinant fragment recognition by
ICM - derived antibodies was delimited to three regions 150 - 200 residues long , with seven of the
octapeptide epitopes also mapping to these regions . -
pubmed
Morphine ( 10 mg / kg ) ,
D , L - amphetamine ( 4 mg / kg ) and
riluzole ( 4 mg / kg ) itself each produced a significant
CPP ; however , when
riluzole was co - administered with
morphine or
amphetamine during the conditioning sessions , no
CPP developed . -
pubmed
The purpose of this study was to investigate the
CYP isoform specificity of
oxybutynin N - deethylation and possible interactions . -
pubmed
Recombinant
CYP3A5 enzyme had higher activity in
oxybutynin N - deethylation than recombinant
CYP3A4 . -
pubmed
Ketoconazole inhibited
oxybutynin N - deethylation by the recombinant
CYP3A4 and
CYP3A5 almost completely , whereas
itraconazole inhibited the activity of
CYP3A4 more potently than that of
CYP3A5 . -
pubmed
To investigate the properties of the two
RAG1.RAG2 complexes in the presence of different
Me2 + cofactors , we established an in vitro
Mg2 + -based cleavage reaction on a single
RSS . -
pubmed
In contrast ,
Mn2 + allows aberrant binding of
RAG1.RAG2 to single - stranded
RSS and permits cleavage independent of binding to the nonamer . -
pubmed
To determine the contribution of
Me2 + ions at the early stages of V(D)J recombination , we analyzed specific DNA recognition and cleavage by
RAG1.RAG2 on phosphorothioated substrates . -
pubmed
These experiments revealed that
Me2 + ions directly coordinate the binding of
RAG1.RAG2 to the
RSS DNA . -
pubmed
All doses of
bFGF used in this study suppressed synthesis of
chondroitin 6 - sulfate . -
pubmed
PKC alpha and
PKC delta are activated and translocated to the nucleus upon exposure of cells to
calcitriol . -
pubmed
Accumulating evidence suggests that
angiotensin -( 1 - 7 )( Ang -( 1 - 7 ) ) is an important component of the
renin -
angiotensin system and that the actions of the peptide may either contribute to or oppose those of
Ang II . -
pubmed
Both
neprilysin and
thimet oligopeptidase are also involved in the metabolism of
bradykinin and the
atrial natriuretic peptide . -
pubmed
Moreover , recent studies suggest that in addition to
Ang I and
bradykinin ,
Ang -( 1 - 7 ) is an endogenous substrate for
angiotensin converting enzyme . -
pubmed
Thus ,
Ang -( 1 - 7 ) may oppose the actions of
Ang II directly or as a result of increasing
prostaglandins or
nitric oxide . -
pubmed
, glycation ) of proteins , which results in abnormal aggregation of
collagen fibrils and production of
superoxide radicals . -
pubmed
The metabolite
GLP-1 ( 9 - 36 )
amide was followed in blood , lung , and kidney . -
pubmed
At 24 - h
post - seeding interval , the number of cells which adhered to the ion - modified
polystyrene was significantly higher than on the unmodified material ( +20 and +58 % in cultures with the
polystyrene irradiated by lower and higher ion doses , respectively ) . -
pubmed
As was shown by ELISA , the
polystyrene irradiated by the higher ion dose enhanced the expression of a cytoskeletal protein ,
vimentin ( +65 % ) and protein of focal adhesion plaques ,
talin ( +15 % ) . -
pubmed
TdT - mediated
dUTP -
biotin nick end - labeling ( TUNEL ) and enzyme histochemistry for
acid phosphatase were performed in both models and results were analyzed by light microscopy , electron microscopy , and confocal laser scanning microscopy ( CLSM ) . -
pubmed
jararacussu bothropstoxins - I (
BthTX - I ) and
II (
BthTX - II ) were submitted to chemical modifications of :
His , by
4 - bromophenacyl bromide (
BPB ) or photooxidation by Rose Bengal ( RB ) ;
Tyr , by
2 - nitrobenzenesulphonyl fluoride (
NBSF ) ; and
Trp , by
o - nitrophenylsulphenyl chloride (
NPSC ) . -
pubmed
The
myotoxic and cytotoxic activities of
BthTX - I , a Lys49 PLA(2 ) homologue , after modification by
BPB , RB , NBSF and NPSC , were reduced to 50 % , 20 % , 75 % , 65 % and 13 % , 0.5 % , 76 % , 58 % , respectively . -
pubmed
BPB - treated
BthTX - II , an Asp49 PLA(2 ) homologue , lost most of its catalytic , indirect hemolytic , anticoagulant ,
myotoxic and cytotoxic activities . -
pubmed
Lethality caused by
BthTX - I and - II was strongly reduced after treatment with
BPB or RB , but only partially with
NBSF or
NPSC . -
pubmed
Serum
creatinine ( SCr ) ,
TSH , FT4 and other chemicals were compared before and 2 mo after
L - thyroxine (
LT4 ) substitution therapy . -
pubmed
PATIENTS AND METHODS : Three groups of patients with at least one colorectal
adenoma were included in a case control study : 401 heavy drinkers ( group HD , mean daily
alcohol intake 117 ( SD 4 ) g / day for a mean duration of 22 ( SD 0.6 ) years ) , aged 57 ( 0.5 ) years ( 78 % men ) ; 152 patients suffering from
irritable bowel syndrome (
IBS ) , aged 61 ( 0.9 ) years ( 57 % male ) ; and 108 patients with a
family history (
FH ) of colorectal
adenoma or
cancer , aged 55 ( 1 ) years ( 64 % male ) . -
pubmed
Cytomegalovirus UL97 phosphotransferase mutations that affect susceptibility to
ganciclovir . -
pubmed
Low drug levels resulting from oral
GCV therapy may predispose the virus to the initial selection of these low - grade
UL97 resistance mutations and to later accumulation of other mutations and greater resistance . -
pubmed
For
arsenic speciation a variety of mass spectrometric techniques , mainly inductively coupled plasma mass spectrometry ( ICP -
MS ) and electrospray tandem mass spectrometry ( ES -
MS /
MS ) coupled on - line with high - performance liquid chromatography ( HPLC ) , are in use . -
pubmed
Product ion scans with multiple stages of tandem
MS ( MS(n ) ; n=2 - 4 ) were used to acquire diagnostic data for each
arsenosugar . -
pubmed
The coding sequence fused to the cleavable glycoprotein signal peptide
gp 64 , and a C - terminal
histidine tag was placed under the transcriptional control of the
Orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate - early 2 ( OpIE2 ) promoter . -
pubmed
Gender- and race - specific determination of
albumin excretion rate using
albumin - to -
creatinine ratio in single , untimed urine specimens : the Coronary Artery Risk Development in Young Adults Study . -
pubmed
Although
albumin excretion rate is commonly estimated by using
albumin /
creatinine ratio ( A / C ) , gender and race differences in
creatinine excretion may bias this estimate . -
pubmed
There are several reports from in vitro studies indicating that
vanadium increases enzyme activity in
insulin signalling pathways ; however , these findings have not been duplicated in vivo . -
pubmed
Insulin dependent
GLUT4 is a major
glucose transporter present in skeletal muscle , adipocytes and heart . -
pubmed
However , with a single dose of
BMOV the
GLUT4 level was restored to normal . -
pubmed
RESULTS : There was a decline of liver
hydroxyline and serum
AST and
ALT in
oxymatrine group compared to those of the
D - GalN group . -
pubmed
The
SOD activity was ( 149.81 15.28 ) NU / mg in
oxymatrine pretreatment group and ( 95.22 16.33 ) NU / mg in the model group ( t=7.309 , P<0.01 ) ; ( 157.68 19.54 ) NU / mg in the treatment group compared with ( 119.88 14.94 ) NU / mg in the model group ( t=4.348 , P<0.01 ) . -
pubmed
faecalis strain V583 and here report the identification of a chromosomal gene ,
katA , encoding a
heme - containing cytoplasmic
catalase . -
pubmed
A
hexahistidyl - tagged version of the
catalase was purified , and major characteristics of the enzyme were determined . -
pubmed
It contains one
protoheme IX group per
KatA polypeptide . -
pubmed
faecalis cells grown in the presence of
heme in the medium ; about 2 and 10 micro M
hemin was required for half - maximal and maximal production of
catalase , respectively . -
pubmed
BIII-890 - CL is a non - orally active
sodium channel blocker under development by Boehringer Ingelheim Corp for the potential treatment of
thromboembolic stroke . -
pubmed
Pharmacological profile of
MEN 11066 , a novel potent and selective
aromatase inhibitor . -
pubmed
No change in
11beta - hydroxylase or
21 - hydroxylase activities was produced in vitro by the addition of 10 microM
MEN 11066 . -
pubmed
These results demonstrated that
MEN 11066 is a potent inhibitor of
aromatase which does not interfere with the
cytochrome P450 involved in the synthesis of other
steroids or in the metabolism of xenobiotics . -
pubmed
Down - regulation of basolateral (
Ntcp ,
Oatp1 , and
Mrp3 ) and canalicular (
Mrp2 ,
Bsep ) transporter mRNA occurred after treatment with
IL-6 ,
turpentine , and LPS . -
pubmed
A novel
methoxy group migration from the
carbonyl group to the
phosphoryl group was observed in ESI -
MS2 . -
pubmed
Biotin synthase is responsible for the synthesis of
biotin from
dethiobiotin and
sulfur . -
pubmed
Although the name of the protein implies that it functions as an enzyme , it has been consistently reported that
biotin synthase produces & lt;1 molecule of
biotin per molecule of protein in vitro . -
pubmed
Functional
COMT variant predicts response to high dose
pyridoxine in
Parkinson ' s disease . -
pubmed
Pyridoxal-5 - phosphate , the biological active form of
pyridoxine , is a cofactor for
dopa - decarboxylase (
DDC ) enzyme . -
pubmed
However , this effect can be negated in the presence of a
DDC inhibitor , which potentiates plasma
levodopa level . -
pubmed
A single
nucleotide polymorphism at the nucleotide 1947 in the
catechol - O - methyltransferase (
COMT ) gene encodes the high (
COMT(H ) ) and low activity (
COMT ( L ) ) forms of the enzyme . -
pubmed
Using a multivariate model , the presence of the
COMT(L ) allele predicted response to
pyridoxine , with the best outcome observed in
COMT(L / L ) homozygotes . -
pubmed
This study aims to estimate the tolerable
lactose intake which can be utilized in the digestion by
lactase and in the fermentation by intestinal microbes in Japanese female adults . -
pubmed
A second
lactose tolerance test involving 10 g and 30 g of
lactose was carried out in 10 subjects showing resistance to
diarrhea , and serum
glucose and
insulin levels and the amount of
hydrogen excreted in the breath were measured for comparison with those of
glucose and
lactitol . -
pubmed
Serum
glucose and
insulin levels were scarcely elevated following the ingestion of both 10 g and 30 g of
lactose , while the amount of
hydrogen excreted in the breath was greatly increased following the ingestion of 30 g of
lactose , but these levels were less following the ingestion of 10 g of
lactose . -
pubmed
In contrast , the ingestion of 15 g of
glucose significantly increased blood
glucose and
insulin levels , while no
hydrogen was detected in the breath . -
pubmed
The digestive capacity of
lactase is less than 10 g of
lactose by single ingestion , while intestinal microflora are able to ferment approximately 20 - 30 g of
lactose . -
pubmed
The ability of
aluminum to inhibit the (
Na (+)/ K (+)) ATPase activity has been observed by several investigators . -
pubmed
However , no studies have focused on determining the change in (
Na (+)/ K (+)) ATPase isoforms caused by chronic exposure to
aluminum and its relation with
aluminum toxicity . -
pubmed
Aluminum accumulation upon chronic dietary
AlCl ( 3 ) administration significantly decreased the (
Na (+)/ K (+)) ATPase activity measured in the presence of nonlimiting
Mg -
ATP concentrations , without compromising protein expression of alpha - subunit isoforms in brain and kidney . -
pubmed
Aluminum - induced synaptosomal (
Na (+)/ K (+)) ATPase inhibition was due to a reduction in the activity of isozymes containing
alpha ( 1 )- alpha ( 2 ) and alpha(3)-subunits . -
pubmed
The onset of enzyme inhibition was accompanied by a decrease of the (
Na (+)/ K (+)) ATPase sensitivity to submicromolar concentrations of
ouabain , and it preceded major damage in plasma membrane integrity and energy supply , as revealed by the analysis of
lactate dehydrogenase leakage and endogenous
adenine nucleotides . -
pubmed
The data suggest that , during chronic dietary exposure to
AlCl ( 3 ) , brain (
Na (+)/ K (+)) ATPase activity drops , even if no significant alterations of catalytic subunit protein expression , cellular energy depletion , and changes in cell membrane integrity are observed . -
pubmed
PKA - dependent and
PKA - independent pathways for
cAMP - regulated exocytosis . -
pubmed
Until recently , it was generally thought that the effects of
cAMP in regulated exocytosis are mediated by activation of
cAMP - dependent protein kinase (
PKA ) , a major
cAMP target , followed by phosphorylation of the relevant proteins . -
pubmed
Although the involvement of
PKA - independent mechanisms has been suggested in
cAMP - regulated exocytosis by pharmacological approaches , the molecular mechanisms are unknown . -
pubmed
Newly discovered
cAMP - GEF /
Epac , which belongs to the
cAMP - binding protein family , exhibits
guanine nucleotide exchange factor activities and exerts diverse effects on cellular functions including hormone / transmitter secretion , cell adhesion , and intracellular
Ca ( 2 + ) mobilization . -
pubmed
cAMP - GEF /
Epac mediates the
PKA - independent effects on
cAMP - regulated exocytosis . -
pubmed
Thus
cAMP regulates and modulates exocytosis by coordinating both
PKA - dependent and
PKA - independent mechanisms . -
pubmed
The species of
phosphatidylethanolamine (
PE ) and
phosphatidylinositol (
PI ) were all detected as the [ M
- H](- ) ions . -
pubmed
A conformationally flexible ,
urea - based tripodal
anion receptor : solid - state , solution , and theoretical studies . -
pubmed
The
receptors are based around a hexasubstituted
aryl core and contain both
urea and
pyridinium functionalities . -
pubmed
Ascorbate - mediated
iron release from
ferritin in the presence of
alloxan . -
pubmed
Release of
iron from
ferritin requires reduction of
ferric to
ferrous iron . -
pubmed
We investigated the ability of
ascorbate to catalyze the release of
iron from
ferritin in the presence of
alloxan . -
pubmed
Incubation of
ferritin with
ascorbate alone elicited
iron release ( 33 nmol/10 min ) and the generation of
ascorbate free radical , suggesting a direct role for
ascorbate in
iron reduction . -
pubmed
Iron release by
ascorbate significantly increased in the presence of
alloxan , but
alloxan alone was unable to release measurable amounts of
iron from
ferritin . -
pubmed
Superoxide dismutase significantly inhibited
ascorbate - mediated
iron release in the presence of
alloxan , whereas
catalase did not . -
pubmed
(- ) ) generated in reaction systems containing both
ascorbate and
alloxan decreased significantly upon addition of
ferritin , suggesting that A. -
pubmed
Although release of
iron from
ferritin and generation of A. -
pubmed
(- ) is the main reductant involved in
ascorbate - mediated
iron release from
ferritin in the presence of
alloxan and that both
dialuric acid and A. -
pubmed
Olmesartan treatment resulted in a significant decrease of BP , urinary protein excretion , plasma
glucose ( 99 + /- 16 versus 92 + /- 14 mg / dl ; P & lt ; 0.05 ) ,
insulin ( 23.1 + /- 8.8 versus 19.9 + /- 9 ; P & lt ; 0.05 ) , HOMA index ( 6.0 + /- 2.7 versus 4.7 + /- 2.8 ; P & lt ; 0.05 ) , and
glycated hemoglobin ( 5.33 + /- 0.58 versus 4.85 + /- 0.81 % ; P & lt ; 0.01 ) . -
pubmed
Oligonucleotides containing a
methylene bridge between
Nl or N9 of the
heterocyclic base and
C1 ' of the
pentofuranosyl ring (
homo - N - oligonucleotides ) were synthesized . -
pubmed
We altered
amino acids in TRM6 and
TRM61 that were predicted to be important for the stability of the heteroligomer based on this model . -
pubmed
These findings , in conjunction with the therapeutic response of
NES to
sertraline , indicate that the
serotonin system is involved in the pathophysiology of
NES . -
pubmed
Electrospray mass spectrometry showed that the modified
enzyme preparation was a mixture of
proteins containing from 2 to 5 covalently linked
PEG chains per subtilisin molecule . -
pubmed
PEG -
subtilisin catalyzed transesterification between
vinyl butyrate and
1 - hexanol was optimum at 0.55
MH ( 2 ) O , while hydrolysis prevailed above 2
MH ( 2 ) O. -
pubmed
Subtilisin powder and
celite - immobilized
subtilisin were more stable and less susceptible to
autolysis than the
PEG - modified
enzyme . -
pubmed
Circular dichroism and intrinsic protein - fluorescence studies showed that the conformation of
PEG - subtilisin did not change as a function of water concentrations between 0 and 9 M. The K(m , app ) value of
PEG - subtilisin for
1 - hexanol was highly influenced by water , which behaved as a competitive inhibitor in the transesterification reaction with an affinity for the
enzyme similar to that of the
alcohol . -
pubmed
Lyoprotectants such as
sorbitol and free
PEG did not influence the activity of
PEG - subtilisin but notably increased the activity of
subtilisin powder and
celite - immobilized
subtilisin . -
pubmed
An expanded
polyQ sequence in the
huntingtin gene is known to cause the
huntingtin protein to aggregate and form intracellular inclusions as disease progresses . -
pubmed
This review focuses on key questions remaining for how the expanded
polyQ sequences affect the aggregation properties of the
huntingtin protein and the corresponding effects on cellular machinery . -
pubmed
Kinetic analysis revealed that the reduction in the AUC of a substrate of
CYP3A4 produced by consecutive administration of an inducer of
CYP3A4 could be approximated by the equation 1/(1 + CRCYP3A4 * ICCYP3A4 ) , where CRCYP3A4 is the ratio of the apparent contribution of
CYP3A4 to the oral clearance of a substrate and
ICCYP3A4 is the apparent increase in clearance of a substrate produced by induction of
CYP3A4 . -
pubmed
The
CRCYP3A4 was calculated for 22 substrates on the basis of the previously reported method from inhibitory DDI studies using a potent
CYP3A4 inhibitor such as
itraconazole or
ketoconazole . -
pubmed
In addition ,
rifampicin was found to be the most potent inducer among the compounds analysed in the present study , with an
ICCYP3A4 value of 7.7 , followed by
phenytoin and
carbamazepine , with values of 4.7 and 3.0 , respectively . -
pubmed
It was indicated that coadministration of
rifampicin ,
phenytoin and
carbamazepine may reduce plasma AUCs to less than half for a broad range of
CYP3A4 substrate drugs , with
CRCYP3A4 values greater than 0.13 , 0.21 and 0.33 , respectively . -
pubmed
Reversal of
P - glycoprotein - mediated multidrug resistance by the murine double minute 2 antagonist
nutlin-3 . -
pubmed
Nutlin-3 is a
MDM2 inhibitor under preclinical investigation as nongenotoxic activator of the
p53 pathway for
cancer therapy . -
pubmed
Effects of
nutlin-3 single treatment were much more pronounced in
p53 wild - type cell lines ( IC(50)s & lt;3 micromol / L ) than in
p53 - mutated cell lines ( IC(50)s & gt;17 micromol / L ) . -
pubmed
In sharp contrast to the expectations ,
nutlin-3 concentrations that did not affect viability of
p53 - mutated cell lines strongly increased the efficacy of
vincristine in
p53 - mutated ,
P - glycoprotein (
P - gp)-overexpressing cell lines ( decrease in IC(50)s 92- to 3,434 - fold ) . -
pubmed
Moreover ,
nutlin-3 reduced efflux of
rhodamine 123 and other fluorescence dyes that are effluxed by
P - gp . -
pubmed
Kinetic studies and investigation of
P - gp - ATPase activity showed that
nutlin-3 is likely to act as a
P - gp transport substrate . -
pubmed
Examination of the
nutlin-3 enantiomers
nutlin-3a and
nutlin-3b revealed that , in contrast to
MDM2 - inhibitory activity that is limited to
nutlin-3a , both enantiomers similarly interfere with
P - gp - mediated drug efflux . -
pubmed
In conclusion ,
nutlin-3 - induced inhibition of
P - gp and
MRP-1 was discovered as a novel anticancer mechanism of the substance in this report . -
pubmed
Effect of the acidic strength on the vapor phase Beckmann rearrangement of
cyclohexanone oxime over the
MFI zeolite : an embedded ONIOM study . -
pubmed
Within the lesion ,
Sox-2 expression increased transiently , while the number of
nestin - positive ependymal cells increased with a concomitant enhancement of proliferation , as indicated by the mitotic markers
Ki67 and
bromo - deoxyuridine . -
pubmed
The mutations responsible for the spontaneous resistance to
streptomycin and
spectinomycin were identified in the
rpsL and
rrs genes , respectively . -
pubmed
Elimination of the
Kv1.3 voltage - dependent
potassium channel gene produces striking changes in the function of the olfactory bulb , raising the possibility that this channel also influences other sensory systems . -
pubmed
In particular , no axonal staining of
Kv1.3 was detected , and most prominent labeling was in structures surrounding the
somata of the principal neurons , suggesting specific localization to the large calyx of Held presynaptic endings that envelop the principal cells . -
pubmed
The presence of
Kv1.3 in presynaptic terminals was confirmed by coimmunolocalization with the synaptic markers
synaptophysin ,
syntaxin , and
synaptotagmin and by immunogold
electron microscopy . -
pubmed
After
GDC embolization , adjunctive therapies , such as ventricular and/or spinal drainage ( 67 % ) , intrathecal administration of
urokinase ( 41 % ) , continuous cisternal irrigation ( 16 % ) , and external decompression ( 16 % ) , were performed . -
pubmed
The total syntheses of tetrapeptides
tubulysins D ( 1 b ) , U ( 1 c ) , and V ( 1 d ) , which are potent
tubulin polymerization inhibitors , are described . -
pubmed
Exogenous
urocortin-2 increased the conditioned fear - induced expression of
c - Fos in the central amygdala ( CeA ) , and parvocellular neurons of the paraventricular hypothalamic nucleus ( pPVN ) , and revealed the effect of conditioned fear in the medial amygdala ( MeA ) . -
pubmed
Elevated levels of
prolactin (
PRL ) ,
oxytocin ,
progesterone and glucocorticoids are characteristics of lactation , and the pronounced fluctuation of these hormones occurring in this phase may play a role protecting the hippocampus . -
pubmed
Arg149 is involved in switching the low affinity , open state of the binding protein
AfProX into its high affinity , closed state . -
pubmed
The structure of a mutant
AfProX protein ( substitution of
Tyr111 with
Ala ) in complex with GB was solved in the open liganded conformation to gain further insight into ligand binding . -
pubmed
We found that a network of
amino acid side chains communicates the presence of GB toward
Arg149 , which increases ligand affinity and induces domain closure of
AfProX. -
pubmed
These results were corroborated by molecular dynamics studies and support the view that
Arg149 finalizes the high - affinity state of the
AfProX substrate binding protein . -
pubmed
The neurorescuing effect of
A68930 ( a potent selective
D ( 1 ) agonist ) and its role on the regulation of hypothalamus - pituitary - adrenal ( HPA)-axis have been investigated . -
pubmed
CUS caused a significant decrease in the number of
TH ir neurons in the striatum , medial forebrain bundle , ventral tegmental area and substansia nigra and
GR in the cortex , striatum and hippocampus as compared to the non - stress controls (
NS ) . -
pubmed
AS enhanced the activities of
superoxide dismutase (
SOD ) and
glutathione peroxidase (
GSH - Px ) in the cortex and striatum , while
CUS reduced the activities of
SOD and
catalase (
CAT ) in the cortex , striatum and hippocampus , when compared with
NS . -
pubmed
Increased
GSH - Px activity , with
reduced glutathione and increased lipid peroxidation was observed in both
AS and CUS in selected brain regions as compared to
NS . -
pubmed
In conclusion , present findings suggest that the stress - induced immunoreactivity of
TH and
GR in distinct brain regions are modulated by
A68930 leading to the normalization of
HPA - axis response . -
pubmed
A68930 also influenced the brain antioxidant machinery probably through the restoration of stress - induced changes in the dopaminergic system and its crosstalk with
GR . -
pubmed
Modified Moore swabs (
MMS ; consisting of a
polyvinyl chloride cartridge filled with gauze ) capture microorganisms within the packed gauze as water flows through the cartridge , while continuous flow centrifugation (
CFC ) uses centrifugation to sediment the microorganisms while water continuously flows in the system . -
pubmed
By incorporating polymer
electrolyte into the carefully selected
HP system ,
electrolyte layers / channels with length scales of a few tens of nanometers to micrometers can be formed with controlled orientation and anisotropy ; ionic conductivity anisotropy as high as 37 has been achieved . -
pubmed
To evaluate the contribution of base stacking to the incorporation efficiency of
dNTPs when a
DNA polymerase encounters an abasic site , we varied the penultimate base pairs ( PBs ) adjacent to the abasic site using all 16 possible combinations . -
pubmed
The efficiency of incorporation for incoming
dNTPs opposite an abasic site varied between 2- and 210 - fold depending on the identity of the
PB . -
pubmed
Crystal structures of the ternary complexes show that the surface of the incoming base was stacked against the
PB 's interface and that the kinetic parameters for
dNMP incorporation were consistent with specific features of base stacking , such as surface area and partial charge - charge interactions between the incoming base and the
PB . -
pubmed
Without a templating
nucleotide residue , an incoming
dNTP has no base with which it can hydrogen bond and can not be desolvated , so that these surrounding water molecules become ordered and remain on the
PB 's surface in the ternary complex . -
pubmed
When these water molecules are on top of a hydrophobic patch on the
PB , they destabilize the ternary complex , and the incorporation efficiency of incoming
dNTPs is reduced . -
pubmed
Vectors with dominant selective markers for
G418 ,
hygromycin B ,
zeocin , and
rho - fluoro - DL - phenylalanine were used to successfully transform
JP1 cells . -
pubmed
Promastigotes of
Deltaatg4.2 but not
Deltaatg4.1 were more susceptible than wild type promastigotes to
starvation and oxidative stresses , which correlated with increased reactive
oxygen species levels and oxidatively damaged proteins in the cells as well as impaired mitochondrial function . -
pubmed
CONCLUSIONS : The results of this study suggest that
bevacizumab is not promising for clinical implementation in the setting of HIPEC : the effects on
tumour size were ambivalent and there was no statistically significant benefit in
TGD . -
pubmed
Vesicular monoamine transporter type 2 (
VMAT2 ) proteins in nerve terminals uptake
dopamine into synaptic vesicles , preventing its cytoplasmic accumulation and toxic damage to nigral neurons . -
pubmed
Presence of the
JAK2 V617F mutation in a patient with
chronic neutrophilic leukemia and effective response to
interferon alpha-2b . -
pubmed
We used a modified staining approach with a single
MHC Class I tetramer ( K(d ) ) bound to two different fluorochromes to discriminate between the Class I - binding and
fluorochrome - streptavidin - binding B cells with a high degree of specificity and binding efficiency . -
pubmed
coli
isobutanol tolerance through engineering its global
transcription factor cAMP receptor protein (
CRP ) . -
pubmed
coli
isobutanol tolerance from its transcriptional level by engineering its global transcription factor
cAMP receptor protein (
CRP ) . -
pubmed
Random mutagenesis libraries were generated by error - prone PCR of
crp , and the libraries were subjected to
isobutanol stress for selection . -
pubmed
Variant
IB2 ( S179P , H199R ) was isolated and exhibited much better growth ( 0.18 h(-1 ) ) than the control ( 0.05 h(-1 ) ) in 1.2 % ( v / v )
isobutanol ( 9.6 g / L ) . -
pubmed
Genome - wide DNA microarray analysis revealed that 58 and 308 genes in
IB2 had differential expression ( & gt;2 - fold , p & lt ; 0.05 ) in the absence and presence of 1 % ( v / v )
isobutanol , respectively . -
pubmed
When challenged with
isobutanol , genes related to acid resistance (
gadABCE ,
hdeABD ) ,
nitrate reduction (
narUZYWV ) , flagella and fimbrial activity (
lfhA ,
yehB ,
ycgR ,
fimCDF ) , and
sulfate reduction and transportation (
cysIJH ,
cysC ,
cysN ) were the major functional groups that were up - regulated , whereas most of the down - regulated genes were enzyme (
tnaA ) and transporters (
proVWX ,
manXYZ ) . -
pubmed
As demonstrated by single - gene knockout experiments ,
gadX ,
nirB ,
rhaS ,
hdeB , and
ybaS were found associated with
strain isobutanol resistance . -
pubmed
The intracellular reactive
oxygen species ( ROS ) level in
IB2 was only half of that of the control when facing stress , indicating that
IB2 can withstand toxic
isobutanol much better than the control . -
pubmed
The normalized specific flux through the
MBR and
RO membranes was evaluated along with inorganic and organic constituents in the influent and effluent of each process . -
pubmed
Increasing the SRT in the
MBR led to an increase in the removal of bulk
DOC , protein , and
carbohydrates , as has been observed in previous studies . -
pubmed
However , the opposite trend was observed for fouling of the
RO membranes ; increasing the SRT of the
MBR resulted in increased fouling of the
RO membranes . -
pubmed
These results indicate that the constituents that foul
MBR membranes are not the same as those that foul
RO membranes ; to be an RO membrane foulant in a
MBR - RO system , the constituents must first pass through the
MBR membranes without being retained . -
pubmed
Gene deletion analyses confirmed that the
enoyl - CoA hydratase / dehydrogenase Fox2p , the putative
3 - hydroxypropionyl - CoA hydrolase Ehd3p , the
3 - hydroxypropionate dehydrogenase Hpd1p , and the putative
malonate semialdehyde dehydrogenase Ald6p essentially contribute to
propionyl - CoA degradation and its conversion to
acetyl - CoA. -
pubmed
The function of
Hpd1p was further supported by the detection of accumulating
3 - hydroxypropionate in the
hpd1 mutant on
propionyl - CoA - generating nutrients . -
pubmed
Substrate specificity of
Hpd1p was determined from recombinant purified enzyme , which revealed a preference for
3 - hydroxypropionate , although
serine and
3 - hydroxyisobutyrate could also serve as substrates . -
pubmed
Nutritional status was assessed from
serum albumin ,
urea ,
creatinine ,
Hb ,
ferritin , and
folate and plasma
amino acid concentrations at 4 months . -
pubmed
Intra - PVN administration of H3 ( 1.8 - 1620 pmol ) significantly increased plasma
ACTH at 1620 pmol H3 and
corticosterone at 180 - 1620 pmol H3 at 30 min following injection compared with vehicle . -
pubmed
Treatment of hypothalamic explants with H3 ( 10 - 1000 nM ) stimulated the release of
corticotrophin - releasing hormone (
CRH ) and
arginine vasopressin (
AVP ) , but
H3 had no effect on the release of
ACTH from in vitro pituitary fragments . -
pubmed
These results suggest that
relaxin-3 may regulate the
HPA axis , via hypothalamic
CRH and
AVP neurons . -
pubmed
ISG activation depends on
Tat interaction with
MAP2K3 ,
MAP2K6 , and
IRF7 promoters and a single exon
Tat protein more strongly modulated the
luciferase activity mediated by
MAP2K3 ,
MAP2K6 , and
IRF7 promoter sequences located 5 ' of the RNA start site than the wild type two - exon
Tat , while a
cysteine and
lysine Tat mutants , reduced in LTR transactivation , had negligible effects on these promoters . -
pubmed
Lithium treatment induced
somatic growth retardation , renal microcysts and dilatations in cortical collecting duct ; it increased cortical cell proliferation and inactive
pGSK-3beta abundance ; it lowered
aquaporin-2 (
AQP2 ) protein abundance and induced
polyuria with decreased ability to concentrate the urine ; and it increased
COX-2 protein level in thick ascending limb . -
pubmed
Concomitant treatment with
lithium and a specific
COX-2 inhibitor ,
parecoxib ( 5 mg / kg per day , P10 -
P34 ) , did not prevent
lithium - induced
microcysts and
polyuria , but improved urine concentrating ability transiently after a
1 - desamino-8 - D - arginine vasopressin challenge . -
pubmed
COX-2 inhibition did not reduce cortical
lithium - induced cell proliferation and phosphorylation of
glycogen synthase kinase-3beta (
GSK-3beta ) . -
pubmed
Discovery of
( S )- 1 -( 1 -( Imidazo [ 1,2 - a ] pyridin-6 - yl ) ethyl )- 6 -( 1 - methyl-1H - pyrazol-4 - yl )- 1H -[ 1,2,3 ] triazolo [ 4,5 - b ] pyrazine (
volitinib ) as a highly potent and selective
mesenchymal - epithelial transition factor (
c - Met ) inhibitor in clinical development for treatment of
cancer . -
pubmed
Different non - ionic cellulose ethers ( methyl cellulose , MC ; hydroxyethyl cellulose ,
HEC ;
hydroxypropyl cellulose , HPC ; hydroxypropylmethyl cellulose , HPMC ) and
microcrystalline cellulose (
MCC ) were investigated as matrix formers for preparation of mini - tablets targeting vaginal drug delivery . -
pubmed
Hexyl aminolevulinat hydrochloridum (
HAL ) was used as a model drug . -
pubmed
PSi layers were synthesized using a two - electrode
Teflon electrochemical cell ; the etching solution contained
hydrogen peroxide 30 % ,
hydrofluoric acid 40 % (
HF ) , and
methanol . -
pubmed
These
Si - ncs colloidal solutions , measured by photoluminescence (
PL ) , revealed efficient blue - green or violet emission intensities . -
pubmed
After the experimental periods , blood samples were collected for measurement of
oestradiol ,
calcium ,
phosphorus and
alkaline phosphatase . -
pubmed
On day 7 ,
alkaline phosphatase activity ,
calcium and
phosphorus were higher in the OVX - RLX group than in the OVX - veh group ( P & lt ; 0.001 ) , but immunolabelling for
RANKL and
HIF-1alpha was lower in OVX - RLX group ( P & lt ; 0.001 ) . -
pubmed
Mitochondrial
iron accumulation exacerbates
hepatic toxicity caused by
hepatitis C virus core protein . -
pubmed
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT : Elevated rates of
substance use disorders (
SUDs ) , particularly ( but not only )
alcohol use , are observed among
post -
weight loss surgery (
WLS ) patients . -
pubmed
GlyR function is affected by a variety of allosteric modulators including drugs of abuse , such as
ethanol and inhalants and the ubiquitous divalent cation
zinc . -
pubmed
A direct comparison of agonist concentration - response curves showed that
zinc enhancement was greater , overall , for
taurine - activated than
glycine - activated receptors . -
pubmed
In addition ,
zinc only enhanced
taurine- but not
glycine - activated
GlyR when agonists were applied at saturating concentrations . -
pubmed
These data suggest that
zinc affects
taurine affinity , as well as the probability of channel opening at sub - maximal
taurine concentrations , and that the magnitude of allosteric modulation at the
GlyR depends on the efficacy of the agonist tested . -
pubmed
Cloning , purification , crystallization and preliminary X - ray studies of a
carbohydrate - binding module from family 64 (
StX ) . -
pubmed
In this context , the study of
carbohydrate - binding modules ( CBMs ) , which are involved in guiding the catalytic domains of
glycoside hydrolases to polysaccharides , is crucial for enzyme development . -
pubmed
We recently showed using the
beta - arrestin - biased beta - blocker ,
carvedilol that
beta - arrestin1 - biased
beta1 - adrenergic receptor cardioprotective signalling stimulates the processing of miR-150 in the heart . -
pubmed
Supported lipid bilayers on glass coverslips that contain
phosphatidylserine and
phosphatidylinositol 4,5 - bisphosphate (
PI ( 4,5 ) P2 ) make
actin bundles termed filopodia - like structures that contain
fascin and have
vasodilator - stimulated phosphoprotein (
VASP ) at their growing tips , indicating that these resemble filopodia growing from the plasma membrane . -
pubmed
The combination of
PI ( 4,5 ) P2 and
phosphatidylinositol 3 - phosphate in curved liposomes or supported bilayers on glass nanospheres uses
Snx9 ,
Cdc42 ,
N - WASP (
neuronal - Wiskott - Aldrich syndrome protein ) , and
Arp2 / 3 complex for
actin polymerization suggesting that this membrane may mimic the progression from plasma membrane to endosomes . -
pubmed
Here we describe how to prepare high - speed supernatant frog egg extracts and
phosphoinositide - containing liposomes and supported lipid bilayers that can assemble
actin structures . -
pubmed
The chemical structures of
P1 A was identified by complete acid hydrolysis , partial acid hydrolysis ,
periodate oxidation - Smith degradation , methylation analysis , IR and NMR . -
pubmed
The results showed that
P1 A had a backbone consisting
rhamnose ,
mannose ,
glucose and
galactose . -
pubmed
Fatty acid (
FA ) -sensitive neurons are present in the brain , especially the hypothalamus , and play a key role in the neural control of energy and
glucose homeostasis including feeding behavior , secretion
insulin and action . -
pubmed
Molecular effectors of these
FA effects include ion channels such as
chloride ,
potassium or
calcium . -
pubmed
Developmental exposure to
bisphenol A alters expression and DNA methylation of
Fkbp5 , an important regulator of the stress response . -
pubmed
The
estrogen receptor (
ER ) antagonist
ICI 182,780 or
ERbeta knock - down affected
Fkbp5 expression and methylation similarly to
BPA . -
pubmed
Further ,
BPA 's effect on
Fkbp5 was abolished upon knock - down of
ERbeta , suggesting a role for this receptor in mediating
BPA 's effects on
Fkbp5 . -
pubmed
Decreasing in retrogradation was observed in both
NRF and GRF with
MPP added of all levels . -
pubmed
The
hydrogen bonds and hydrophilic interactions between
phenolic compounds with amylopectin molecule may be involved the decrease of starch retrogradation , especially
GRF . -
pubmed
Assessment of 25
CYP2D6 alleles found in the Chinese population on
propafenone metabolism in vitro . -
pubmed
The aim of this study was to assess the catalytic characteristics of 25 alleles (
CYP2D6.1 and 24
CYP2D6 variants ) and their effects on the metabolism of
propafenone in vitro . -
pubmed
The levels of SC and other laboratory indexes including complete blood counts , leukocytes , the immature - to - total - neutrophil ratio ,
procalcitonin (
PCT ) ,
C - reactive protein , and blood
lactate were detected ,
cytokines [
interleukin ( IL )- 2 ,
IL-6 , and
interferon - gamma ] released by neutrophils were also determined . -
pubmed
Moreover ,
SAF plants exhibited enhanced drought stress tolerance and better growth under drought stress treatment , which was accompanied by a reduced transpiration rate and lower reactive
oxygen species contents . -
pubmed
Randomized Trial of the Effect of
Magnesium Sulfate Continuous Infusion on
IL-6 and
CRP Serum Levels Following
Abdominal Aortic Aneurysm Surgery . -
pubmed
However ,
IL-6 level was significantly less in intervention group early after the end of
MgSO4 infusion comparing with control group ( P = 0.01 ) . -
pubmed
According to the results of this study , continuous infusion of
MgSO4 after
AAA surgery may provide
IL-6 suppression . -
pubmed
DNA - mediated assembly of core - satellite structures composed of
Zr ( IV )-based
porphyrinic metal - organic framework (
MOF ) and
NaYF4 ,
Yb ,
Er upconverting nanoparticles ( UCNPs ) for photodynamic therapy ( PDT ) is reported . -
pubmed
Under NIR irradiation , visible light emitted from the UCNPs is absorbed by the core
MOF NP to produce 1
O2 at significantly greater amounts than what can be produced from simply mixing UCNPs and
MOF NPs . -
pubmed
Sugars with heteroatoms other than
oxygen have attained considerable importance in glycobiology and in drug design since they are often more stable in blood plasma due to their resistance to enzymes , such as
glycosidases ,
phosphorylases and
glycosyltransferases . -
pubmed
MTT assay showed that si -
NEAT1 remarkably inhibited the cell proliferation in three
HCC cell lines . -
pubmed
In this Account , we extend the GPF -
EP method to describe bonds involving
N electrons in M orbitals ( N & lt ; M ) and show its application to ( 2c1e ) bonds . -
pubmed
The method is employed to generate new characteristics of the B11u(51P1 ) electronic state of
Cd2 based on
LIF excitation spectrum previously recorded using the B11u X10g+ ( 51S0 ) transition . -
pubmed
Correctness of the method is additionally tested on artificially generated
LIF excitation spectra based on well characterized
b30u +( 53P1 ) X10g+ transition in
Cd2 . -
pubmed
INTRODUCTION : To compare the percentage of arterial
oxygen saturation ( SpO2 ) in healthy teeth with confirmed pulp vitality between individuals with
sickle cell anemia (
HbSS ) and normal
hemoglobin A (
HbAA ) . -
pubmed
Furthermore expression of the enzyme responsible for
PGD2 synthesis (
hematopoietic prostaglandin D synthase ,
HPGDS ) was increased in
obese adipose tissues and remained high after
weight loss . -
pubmed
Perioperatively ,
Levobupivacaine hydrochloride ( 15 mg ) was administered in the subarachnoid space , at the
L4 -
L5 lumbar intervertebral space , using a 29 Gauge needle . -
pubmed
The results showed that long - term exposure to 1 mg / L
CeO2 NPs induced the deterioration of denitrifying process , which was consistent with the inhibition of enzyme activities of
nitrite oxidoreductase and
nitrate reductase under
CeO2 NPs . -
pubmed
To test the efficacy of
nicotine patch preloading for engaging proposed treatment targets during the pre - quit phases , we used multilevel models to compare
post - smoking changes in symptoms and cravings during the preloading phases to post - smoking changes reported during the ad lib smoking phase . -
pubmed
RESULTS : There was no significant difference in quit rates across the two conditions on the primary outcome of seven - day point prevalence smoking abstinence bioverified with breath
carbon monoxide at six weeks
post - quit date . -
pubmed
We show that ANANAS tethering of
cetuximab promotes a more efficient
EGFR - dependent vesicle - mediated internalization . -
pubmed
Broadband dielectric relaxation spectroscopy (
DRS ) has been used to investigate aqueous solutions of
MgCl2 and
CaCl2 up to concentrations of about 1.8 mol L-1 at 25 C over the frequency range 0.07 & lt;= nu / GHz & lt;= 89 . -
pubmed
Detailed analysis of the dominant solvent mode centered at ~20 GHz showed that both
Mg2 + and
Ca2 + are strongly solvated , each immobilizing ~20 water molecules on the
DRS timescale . -
pubmed
Thus ,
resveratrol as a
hypoxia - inducible factor-1 - alpha inhibitor increased the radiosensitization of
breast cancer spheroid cells . -
pubmed
4 - Nonylphenol - enhanced
EZH2 and
RNF2 expression , H3K27me3 and
H2AK119ub1 marks resulting in silencing of
p21CDKN1A in vitro . -
pubmed
The
phospho -
CREB ,
SP1 and
E2F-1 are enriched at proximal promoter of
RNF2 and
EZH2 and
cyclin D1 , but not
p21 . -
pubmed
In 2018 , The Food and Drug Administration ( FDA ) of the US approved several novel agents for
AML treatment as follows :
ivosidenib , an inhibitor of
isocitrate dehydrogenase-1 ;
venetoclax , a potent inhibitor of
bcl2 ; and
glasdegib , an inhibitor of
hedgehog signaling pathway . -
pubmed
Moreover , clinical trials of
alvocidib (
flavopiridol ) , an inhibitor of the
CDK9 ,
pevonedistat , an inhibitor of
NEDD8 , and
APR-246 , a reactivator of mutant
p53 , are in progress . -
pubmed
Multidisciplinary care by an NST leads to fewer complications including
infection and
electrolyte disturbances , and better survival for patients receiving short- and long - term
PN . -
pubmed
Regarding
infection , resorbable presented an absolute risk (
AR ) = 0.032 , and
titanium an
AR = 0.025 ( P = 1.0 ) . -
pubmed
For soft tissue reaction , an
AR = 0.120 was shown for resorbable , and an
AR = 0.132 for
titanium ( P = 0.85 ) . -
pubmed
Removal of fixation showed an
AR = 0.024 for resorbable , and an
AR = 0.025 for
titanium ( P = 1.0 ) . -
pubmed
In this study , we demonstrated that
glycol chitosan (
GC ) and oxidized hyaluronate ( OHA ) can be used to form a self - healing ferrogel in the presence of superparamagnetic
iron oxide nanoparticles ( SPIONs ) without additional chemical cross - linkers . -
pubmed
The overall characteristics of
GC /
OHA / SPION ferrogel varied based on the
GC /
OHA ratio , SPION content , and total polymer concentration . -
pubmed
Interestingly ,
GC /
OHA / SPION ferrogel was used to fabricate 3D - printed constructs of various shapes via an extrusion printing method . -
pubmed
Experience with Teaching Different Modeling Techniques on the example of
Glucose Insulin Regulation Model . -
pubmed
Hands - on implementation using all these techniques and comparing them towards same system ( in our case
glucose -
insulin regulation ) we teach pros and cons of each technique . -
pubmed
Fingolimod induces sequestration of lymphocytes in lymphoid organs by altering lymphocyte expression of
sphingosine-1 - phosphate receptors . -
pubmed
Major
depressive disorder (
MDD ) is associated with alterations of GABAergic interneurons , notably
somatostatin (
Sst ) as well as
parvalbumin (
Pvalb ) , in cortical brain areas . -
pubmed
Chemogenetic inhibition of all
GABA interneurons (
Gad1 + ) , as well as
Sst+ and
Pvalb+ subtypes in the mPFC produced dose and time - dependent antidepressant effects in the forced swim and novelty suppressed feeding tests , and increased synaptic plasticity . -
pubmed
In contrast , stimulation of
Gad1 ,
Sst , or
Pvalb interneurons in mPFC abolished the effects of
scopolamine and prevented
scopolamine induction of synaptic plasticity . -
pubmed
An immunofluorescence microscopy analysis was used to investigate cellular oxidative stress and DNA damage using
DCFDA fluorescence staining and an anti -
gammaH2AX antibody . -
pubmed
Full - dimensional quantum dynamics study of isotope effects for the
H2 +
NH2 /
ND2 /
NHD and
H2 /
D2 /
HD +
NH2 reactions . -
pubmed
After elution from the
antibody column at pH 11 the normal beta component , by dialysis against
pyridoxal phosphate at pH 7.5 , could be restored to the enzymatically - active
beta2 dimer with a specific activity of 1700 enzyme units / mg
protein . -
pubmed
[ The effect of
chloramphenicol on in vitro colony forming cell (
CFC ) ( author 's transl ) ] . -
pubmed
Glutamate responses showed similarities to those of the
kainate / quisqualate receptor subtype . -
pubmed
The reflex
tracheal dilatations were abolished by the combination of intra - arterial
propranolol ( 100 micrograms ) and transections of both the bilateral superior laryngeal nerves and the spinal cord at the
C1 level . -
pubmed
Insulin neither affected basal lipolysis nor inhibited
dibutyl cAMP - stimulated lipolysis . -
pubmed
Immunoprecipitation studies showed that all four
IgG isotypes appear to recognize a similar set of
antigens . -
pubmed
Cytoplasmic tail deletion converts membrane
immunoglobulin to a
phosphatidylinositol - linked form lacking signaling and efficient antigen internalization functions . -
pubmed
Transfected constructs lacking the normal three -
amino acid cytoplasmic tail are expressed exclusively as
phosphatidylinositol - linked proteins , lack both antigen presentation and signal transduction functions , and are internalized slowly following
anti - Ig binding . -
pubmed
The molecular mass of the cytoplasmic tail - deleted
phosphatidylinositol - linked
Ig molecule is consistent with cleavage of the transmembrane residues during processing . -
pubmed
The
sugar - binding sites of
LOLI are occupied by four well - ordered water molecules each . -
pubmed
Fluorescein isothiocyanate specifically modifies
lysine 338 of
cytochrome P-450scc and inhibits
adrenodoxin binding . -
pubmed
Treatment of
cytochrome P-450scc with
fluorescein isothiocyanate (
FITC ) resulted in covalent labeling with 1.0 + /- 0.1 eq of
FITC . -
pubmed
The modification of
lysine 338 with
FITC resulted in 85 + /- 15 % inhibition of
adrenodoxin binding to
cytochrome P-450scc . -
pubmed
In a complementary experiment it was found that if a complex between
adrenodoxin and native
cytochrome P-450scc was formed in the presence of
cholesterol and then treated with
FITC , there was almost no labeling of
lysine 338 . -
pubmed
These experiments suggest that
lysine 338 is located at the binding site for
adrenodoxin and electrostatically interacts with one of the
carboxylate groups on
adrenodoxin that has been implicated in binding . -
pubmed
The fluorescence emission of the
FITC label on
cytochrome P-450scc was only 14 % as large as that of an equivalent concentration of
FITC - labeled
bovine serum albumin , suggesting that it was quenched by Forster energy transfer to the
heme group . -
pubmed
The affinity of the binding sites for
Ins ( 1,4,5 ) P3 was not influenced by the non - hydrolysable
GTP analogues
GTP gamma S and
Gpp [ NH ] p or by preincubation of the
binding protein with a preparation of partially purified
protein kinase C in the presence of
ATP and
TPA (
12 - O - tetradecanoylphorbol 13 - acetate ) . -
pubmed
The variant could not be resolved from
Hb A on cellulose acetate ( pH 8.4 ) , but isoelectric focusing showed a double band in the region of
Hb A that is probably the variant and
Hb A. However , the variant chain was clearly evident by
globin chain analyses in
acid and alkaline buffers . -
pubmed
Chronic
lithium prevets
reserpine - induced supersensitivity of
adeylate cyclase . -
pubmed
We describe a synthetic
oligonucleotide that can prime specifically reverse transcription of
VSG mRNA out of total trypanosome poly(A)+ RNA . -
pubmed
Our medium was a modification of that of Doyle and Roman with the addition of filter - sterilized
FBP ( 0.2 %
ferrous sulfate , 0.025 %
sodium metabisulfite , 0.05 %
sodium pyruvate ) , 0.1 %
sodium lauryl sulfate , and 0.075 % agar . -
pubmed
Thus the total cellular concentrations of both
estradiol and
progesterone receptors are lowest in the late secretory phase . -
pubmed
In anovulatory cycles the concentrations of
estradiol and
progesterone receptors are high , similar to those of the late proliferative phase . " -
pubmed
The dextran - coated
charcoal assay method of McGuire and DeLaGarza is a well - known method for quantitation of
oestrogen receptors in
breast tumour tissues . -
pubmed
The UV spectrum and TSP / LC /
MS /
MS daughter ion analysis led to the proposition of a
bisamide structure for the major metabolite . -
pubmed
TSP / LC /
MS indicated that the molecular weight of the minor metabolite was 16 u higher than the
bisamide , suggesting an oxidized analogue . -
pubmed
Fluorescent choleretic and cholestatic
bile salts take different paths across the hepatocyte : transcytosis of
glycolithocholate leads to an extensive redistribution of
annexin II . -
pubmed
Treatment of couplets with
glycine derivatives of
lithocholate and
ursodeoxycholate , but not
cholate or
chenodeoxycholate , led to a marked relocalization of
annexin II , which initially became concentrated at the basolateral membrane , then moved to a perinuclear distribution and finally to the apical membrane as the incubation progressed . -
pubmed
This suggests that
lithocholate and
ursodeoxycholate treatment leads to a rapid induction of transcytosis and that
annexin II exchange occurs upon membrane fusion at all stages of the hepatocyte transcytotic pathway . -
pubmed
coli strain CA using three independent methods :
SDS gel electrophoresis to quantify the synthesized
luciferase protein ,
EIA to quantify the enzyme native conformer and activity measurements . -
pubmed
The increase in the
enzyme activity correlated well with the increase in the
ATP content in the cell . -
pubmed
Inhibition of
cytokine - primed eosinophil chemotaxis by
nedocromil sodium . -
pubmed
These
cytokines have the capacity to modulate chemotactic responses of eosinophils toward
platelet - activating factor ,
formyl - methionyl - leucyl - phenylalanine , (
FMLP ) and
neutrophil - activating factor (
NAF)/
IL-8 , but not toward complement fragment
C5a (
C5a ) . -
pubmed
CONCLUSIONS : The chemotactic responses toward
C5a were inhibited by
nedocromil sodium at higher concentrations than were required in the priming studies ( IC50 approximately 10 to 100 nmol / L ) . -
pubmed
The
GABAB receptor agonist ,
baclofen , directly hyperpolarized or produced an outward current ( single electrode voltage -
clamp ) in all 66 neurones tested . -
pubmed
2 - OH - saclofen , a selective
GABAB receptor antagonist , competitively antagonized
baclofen - induced hyperpolarizations ( n = 4 ) with equilibrium dissociation constants estimated in two neurones to be 6 and 23 microM. -
pubmed
These studies demonstrate that
baclofen acting on
GABAB receptors increases
potassium conductance in all lateral and ventrolateral periaqueductal gray neurones . -
pubmed
Melatonin and
adrenocorticotropic hormone levels in video display unit workers during work and leisure . -
pubmed
The study investigated possible effects of VDU work on circulating
melatonin and
adrenocorticotropic hormone (
ACTH ) levels . -
pubmed
Circulating
melatonin levels decreased significantly during VDU work , whereas
ACTH levels increased significantly . -
pubmed
Mental
strain during work was significantly and positively associated with circulating levels of
ACTH but not
melatonin . -
pubmed
Mental
strain might explain changes in
ACTH levels , but specific factors that might contribute to changes in
melatonin levels are unknown . -
pubmed
Lines were divergently selected for 4 - wk BW ( HW and LW ) or plasma
TPP (
HP and
LP ) . -
pubmed
The first 5
amino acids of the
carboxyl terminus of
phosphatidylinositol transfer protein ( PITP ) alpha play a critical role in
inositol lipid signaling . -
pubmed
PITP has a single lipid - binding site that can reversibly bind
phosphatidylinositol (
PI ) and
phosphatidylcholine ( PC ) and transfer these lipids between membrane compartments in vitro . -
pubmed
The role of the
carboxyl terminus was examined by comparing wild - type
PITPalpha with
PITPalpha in which 5 , 10 , and 20
amino acids were deleted from the C terminus . -
pubmed
We conclude that ( a ) the
carboxyl terminus of
PITP plays a critical role in
phospholipase C signaling ; ( b ) the transfer activity is not the only determining factor that dictates the restorative function of
PITP in
inositol lipid signaling ; and ( c ) the dominant inhibitory effects of
Delta5- and
Delta10 -
PITP on wild - type
PITP in
phospholipase C signaling suggest the existence of a receptor for
PITP . -
pubmed
PACAP release from islets was bioassayed using the response of cytosolic
Ca2 + concentration ( [
Ca2+]i ) in single beta - cells , monitored by dual - wavelength
fura-2 microfluorometry . -
pubmed
High
glucose - stimulated
insulin release from isolated islets was attenuated by
PACAP antiserum but not by non - immune sera . -
pubmed
The islet incubation medium with high
glucose ( Med ) possessed a capacity , which was neutralized by
PACAP antiserum , to increase [
Ca2 +]i in beta - cells . -
pubmed
PACAP antiserum also neutralized the [
Ca2 +]i - increasing action of synthetic
PACAP-38 and
PACAP-27 , but not that of
vasoactive intestinal polypeptide (
VIP ) and
glucagon . -
pubmed
Both Med and synthetic
PACAP increased [
Ca2 +]i in beta - cells only in the presence of stimulatory , but not basal ,
glucose concentrations . -
pubmed
[
Ca2 +] i measurements combined with immunocytochemistry with
insulin antiserum revealed a substantial population of
glucose - unresponsive beta - cells , many of which were recruited by
PACAP-38 into [
Ca2 +]i responses . -
pubmed
These results indicate that
PACAP-38 is a novel islet substance that is synthesized and released by islet cells and then , in an autocrine and/or paracrine manner , potentiates and arouses beta - cell responses to
glucose , thereby amplifying
glucose - induced
insulin secretion in islets . -
pubmed
The authors measured total T cells , T - helper cells , T - suppressor cells , natural killer cells , and the synthesis of
interleukin-1 ,
interleukin-6 , and
tumor necrosis factor by peripheral blood mononuclear cells before and on Day 40 of
fish oil supplementation . -
pubmed
AMs from animals inhaling asbestos showed significant elevations ( p & lt ; .05 ) in
nitrite /
nitrate levels which were ameliorated by
NG - monomethyl - L - arginine (
NMMA ) , an inhibitor of
inducible nitric oxide synthase (
iNOS ) activity . -
pubmed
Regulation of
phospholipase C delta 1 activity by
GTP - binding proteins :
rhoA as an inhibitory modulator . -
pubmed
Clinical implications of the types of
cryoglobulins determined by two - dimensional
polyacrylamide gel electrophoresis . -
pubmed
Examining the clinical findings in the mono group ( those with monoclonal
IgM alone ) and the poly group ( those with polyclonal
IgM alone or polyclonal and monoclonal
IgM ) we found clearly significant differences : more severe
liver involvement in the poly group , and higher cryocrit and
creatinine values , lower
C4 level and more severe
purpura in the mono group . -
pubmed
SO ( 10 ) operator analysis for nu micro nu
tau oscillations . -
pubmed
Constitutive degradation of
PML / RARalpha through the proteasome pathway mediates
retinoic acid resistance . -
pubmed
Treatment with
retinoic acid ( RA ) induces degradation of
PML / RARalpha , differentiation of leukaemic blasts , and disease remission . -
pubmed
The steady - state ,
dinitrostilbene - disulfonate - sensitive current ( DeltaI ) was
Na + and
HCO3 - dependent and therefore was taken as flux through the
cotransporter . -
pubmed
Our results identify Eph receptors and
ephrins as the first
molecules known to demarcate individual lobules during development . -
pubmed
Membrane topology of the
amino - terminal region of the
sulfonylurea receptor . -
pubmed
The
sulfonylurea receptor (
SUR ) is a member of the
ATP - binding cassette family that is associated with
Kir 6.x to form
ATP - sensitive
potassium channels . -
pubmed
SUR is involved in
nucleotide regulation of the channel and is the site of pharmacological interaction with
sulfonylurea drugs and
potassium channel openers . -
pubmed
SUR contains three hydrophobic domains , TM(0 ) , TM(1 ) , and TM(2 ) , with
nucleotide binding folds following TM(1 ) and TM(2 ) . -
pubmed
We analyzed the topology of the
amino - terminal TM(0 ) region of
SUR1 using glycosylation and
protease protection studies . -
pubmed
Deglycosylation using
peptide - N - glycosidase F and site - directed mutagenesis established that
Asn(10 ) , near the
amino terminus , and
Asn(1050 ) are the only sites of N - linked glycosylation , thus placing these sites on the extracellular side of the membrane . -
pubmed
Restoration by
glycerol of
p53 - dependent apoptosis in cells bearing the mutant
p53 gene . -
pubmed
CONCLUSIONS :
Glycerol appears to function as a chemical chaperone that restores
mp53 to
wtp53 function . -
pubmed
A precise single - point mutation was introduced into genes encoding
acetohydroxyacid synthase (
AHAS ) , at a position known to confer
imidazolinone resistance . -
pubmed
It has been observed that
heparin acts as a specific blocker of
IP3 receptors and inhibits
IP3 - mediated
calcium release in various cell types , including vascular smooth muscle and airway smooth muscle . -
pubmed
The
cationic peroxidases , such as
major basic protein and
eosinophil peroxidase , are neutralized by the highly anionic heparin ; thus
heparin inhibits the epithelial damage induced by some of these cationic proteins . -
pubmed
The
mGluR subtypes that are coupled to the hydrolysis of
phosphoinositide contribute to both synaptic plasticity and
glutamate - mediated excitotoxicity in neurons . -
pubmed
Protein oxidation was estimated by electron spin resonance spectroscopy , after labeling of the ' peroxidized ' mitochondria with
4 - maleimido-2 , 2,6,6 - tetramethylpiperidine-1 - oxyl . -
pubmed
We found that ( i ) low concentrations of
flupirtine ( 10 microM ) protect lipids and also proteins ( with lesser efficiency ) from attacks of reactive
oxygen species ; ( ii )
flupirtine remarkably delayed the decline of complex mitochondrial functions , such as the respiratory control or the
Ca ( 2 + ) retention capacity of mitochondria , under oxidative stress ; and ( iii ) the
ADP / ATP antiporter (
ANT ) , a main component of the oxidative phosphorylation machinery as well as a core component of the permeability transition pore complex , seems to be a membrane protein particularly protected by
flupirtine . -
pubmed
In conclusion , the preservation of the
Ca ( 2 + ) buffer capacity of mitochondria and of the
ANT activity against oxidative stress supports an antiapoptotic application of
flupirtine . -
pubmed
Complex
6.CH ( 2 ) Cl ( 2 ) crystallizes in the triclinic space group
P1 with , at -158 degrees C , a = 13.4635(3 ) , b = 13.5162(3 ) , c = 23.2609(5 ) A , alpha = 84.9796(6 ) , beta = 89.0063(8 ) , gamma = 86.2375(6 ) degrees , Z = 2 , and V = 4207.3(3 ) A(3 ) . -
pubmed
Glutamate is a key transmitter of vagal afferents acting partly on
NMDA receptors . -
pubmed
GABA in the dialysate was determined by high - performance liquid chromatography system and
serum LH concentration was determined by radioimmunoassay . -
pubmed
Conversely , in the
OLN group , elevated
CIC and diminished
CH50 and
C3 serum levels were significantly different ( P & lt ; 0.01 ) . -
pubmed
The membrane - associated
EGF receptors undergo rapid
tyrosine phosphorylation and internalization upon ligand binding in ovarian cells , including luteal cells . -
pubmed
Raf-1 and
B - Raf , but not
A - Raf , were activated by
EGF ( 10 ng / ml ) and the pharmacological
protein kinase C (
PKC ) activator
phorbol myristate acetate (
PMA , 20 nM ) . -
pubmed
Additionally , activated
ERK was found in the nucleus of the cells following treatment with
EGF ( 10 ng / ml ) and
PMA (
PMA , 20 nM ) for 5 min . -
pubmed
Depletion of
PKC by chronic
PMA treatment ( 2.5 microM , 24 h ) only partially inhibited the stimulatory effects of
EGF on
Raf-1 ,
ERK2 and
ERK1 . -
pubmed
Treatment with the
MEK1 inhibitor
PD098059 ( 50 microM ) abolished
EGF - induced
ERK activation . -
pubmed
However , blocking
EGF - induced
ERK activation by pretreatment with
PD098059 only partially attenuated
EGF - induced c - fos and c - jun mRNA expression . -
pubmed
Structural basis for a non -
phosphorus - containing cyclic peptide binding to
Grb2 - SH2 domain with high affinity . -
pubmed
Blocking the interaction between
phosphotyrosine (
pTyr)-containing activated receptors and the
Src homology 2 ( SH2 ) domain of the
growth factor receptor bound protein 2 (
Grb2 ) is considered to be an effective and non - cytotoxic strategy to develop new anti - proliferative agents due to its potential to shut down the
Ras activation pathway . -
pubmed
To understand the structural basis for the high - affinity binding of these novel non -
phosphorus - containing inhibitors to the
Grb2 - SH2 domain , we extensively studied herein the unique functional requirements of the acidic side chain in
Tyr-2 position due to the absence of the
phosphate group in these non - phosphorylated peptides . -
pubmed
With both the peptidomimetic optimization of the
amino acid side - chains and the constraint of the backbone conformation guided by molecular modeling , we developed several potent antagonists with low nanomolar range binding affinity , such as cyclic peptide 20 with an IC(50)=0.026 microM , which is one of the most potent non -
phosphorus - containing
Grb2 -
SH2 antagonists reported to date . -
pubmed
Adenovirus type 2 ( Ad2 ) early region 4 ORF4 (
E4orf4 ) triggers a major
death pathway that requires its accumulation in cellular membranes and its
tyrosine phosphorylation . -
pubmed
Here we provide strong evidence that a physical interaction requiring the kinase domain of
Src and the
arginine - rich motif of
E4orf4 is involved . -
pubmed
METHODS : Baseline PANSS data from six double - blind , randomized registration trials of
olanzapine , three in
schizophrenia and three in acute
bipolar mania , were used in these
post - hoc analyses . -
pubmed
Ciclesonide as a novel
corticosteroid itself is inactive and used as prodrug form and needs to be cleaved by pulmonary
esterases to acquire anti - inflammatory activity . -
pubmed
We use laser flash photolysis and time - resolved Raman spectroscopy of
CO - bound H93
G myoglobin ( Mb ) mutants to study the influence of the proximal ligand on the
CO rebinding kinetics . -
pubmed
In H93 G mutants , where the proximal linkage with the
protein is eliminated and the
heme can bind exogenous ligands ( e.g. -
pubmed
Unlike topical
corticosteroid agents ,
tacrolimus ointment does not cause a reduction in
collagen synthesis or skin thickness . -
pubmed
Because
tacrolimus ointment does not cause
skin atrophy , it may be safely used for months or years on all skin areas , including the
face and intertriginous areas . -
pubmed
Several specific proteins , including
amino acid synthesis enzymes and vacuolar enzymes , are increased during
nitrogen starvation in wild - type cells but not in autophagy - defective delta
atg7 cells despite similar mRNA levels . -
pubmed
Bulk protein synthesis was substantially reduced in delta
atg7 cells under
nitrogen starvation compared with wild - type cells . -
pubmed
The total intracellular
amino acid pool was reduced in delta
atg7 cells , and the levels of several
amino acids fell below critical values . -
pubmed
Compared with the RDR , the sensitivity and specificity to detect
vitamin A deficiency for each test was , respectively : serum
retinol , 90 % and 78 % ;
retinol - binding protein (
RBP ) , 40 % and 91 % ;
retinol / RBP molar ratio , 60 % and 74 % ; conjunctival impression cytology , 44 % and 48 % ; slit - lamp examination , 20 % and 66 % ; tear film break - up time , 40 % and 69 % ; and Schirmer 's test , 20 % and 78 % . -
pubmed
Hyphal uptake of
( 32 ) Pi and expression of plant Pi transporter genes were investigated in the
rmc mutant and its wild - type progenitor ( 76R ) associated with three
AMF . -
pubmed
In all cases , the main fragmentation (
MS2 mode ) of the pseudomolecular ion leads to the neutral loss of a
glutamyl moiety ( -129 Da ) , affording an ion that gives structural information upon an additional fragmentation (
MS3 mode ) . -
pubmed
The
globulin could be separated into two major subunits by
sodiumdodecyl sulfate polyacrylamide gel electrophoresis . -
pubmed
Retinol modulates site - specific mobility of
apo - cellular retinol - binding protein to promote ligand binding . -
pubmed
We have investigated the mechanism of
retinol uptake by the cellular
retinol - binding protein type I (
CRBP ) using line shape analysis of NMR signals . -
pubmed
The highly similar structures of apo- and
holo - CRBP exhibit closed conformations that seemingly offer no access to ligand , yet the protein binds
retinol rapidly and with high affinity . -
pubmed
An initial nonspecific encounter with the ligand induces the formation of long - lived conformers in the portal region of
CRBP suggesting a mechanism how
retinol accesses the cavity . -
pubmed
The
peroxisome proliferation - activated receptor gamma (
PPARgamma ) is mainly expressed in the adipose tissue and integrates the control of energy , lipid , and
glucose homeostasis . -
pubmed
The functionality of the receptor was evidenced by
15 - deoxy-12,14 - prostaglandin J ( 2 ) (
PGJ2 ) and
rosiglitazone (
BRL )
PPARgamma - agonists that were tested on capacitation , acrosome reaction , and motility . -
pubmed
Both treatments also increase
AKT phosphorylations and influence
glucose and lipid metabolism in sperm . -
pubmed
Using transcription reporter assays , we show that single
nucleotide polymorphisms in the
PGIS promoter can affect transcriptional activity . -
pubmed
These results show that an individual 's
PGIS promoter haplotype can play an important role in the predisposition for
lung cancer and
CpG methylation provides an epigenetic mechanism for the down - regulated
PGIS expression . -
pubmed
RESULTS : Bone growth proceeded very rapidly ; at 1 day of age , the degree of mineralization (
phosphate / protein ratio ) , the density of mineralized bone (
TMD ) , and mineral crystallinity had reached 36 % , 51 % , and 87 % of the adult ( 450 days ) values , respectively . -
pubmed
In contrast to the notable changes in mineralization ,
carbonate substitution into the mineral lattice (
carbonate /
phosphate ratio ) and
collagen cross - linking did not show any significant changes over this time period . -
pubmed
Between samples of different time - points , significant positive correlations were observed between the elastic modulus and
TMD ( r(2 ) = 0.84 ) ,
phosphate / protein ratio ( r(2 ) = 0.59 ) , and crystallinity ( r(2 ) = 0.23 ) , whereas
collagen cross - linking showed a small but significant negative correlation ( r(2 ) = 0.15 ) . -
pubmed
The
ATP - binding cassette transporter subfamily A member 1 (
ABC - A1 ) and
type 2 diabetes : an association beyond HDL
cholesterol . -
pubmed
The absence of
ABC - A1 led to
cholesterol accumulation within the beta cell plasma membrane , suggesting that
cholesterol may play a role in the
insulin secretory pathway . -
pubmed
In conclusion ,
ABC - A1 may be more than a determinant of HDL -
cholesterol . -
pubmed
In addition , a profoundly decreased intracellular
calcium flux in
CD4(+)T cells after
PHA stimulus was detected among 620Trp carriers . -
pubmed
Improvement of an
enzyme electrode by
poly ( vinyl alcohol ) coating for amperometric measurement of
phenol . -
pubmed
A
poly ( vinyl alcohol ) film cross - linked with
glutaraldehyde (
PVA -
GA ) was introduced to the surface of a
tyrosinase - based
carbon paste electrode . -
pubmed
The coated
PVA -
GA film was beneficial in terms of increasing the stability and reproducibility of the
enzyme electrode . -
pubmed
The electrode showed a sensitive current response to the reduction of the
o - quinone , which was the oxidation product of
phenol , by the
tyrosinase , in the presence of
oxygen . -
pubmed
The effects of the
PVA and
PVA -
GA coating , the pH , and the
GA :
PVA ratio on the current response were investigated . -
pubmed
The sensitivity of the
PVA -
GA -
Tyr electrode was 130.56microA / mM ( 1.8microA / microM cm(2 ) ) and the linear range of
phenol was 0.5 - 100microM. -
pubmed
Using the
PVA -
GA -
Tyr electrode , a two - electrode system was tested as a prototype sensor for portable applications . -
pubmed
[ Study on the interaction of
hesperidin or
icariin with
lysozyme by fluorescence spectroscopy ] . -
pubmed
The interaction of
hesperidin (
HES ) or
icariin (
ICA ) and
lysozyme (
LYS ) was studied by fluorescence spectroscopy in physiological buffer solution . -
pubmed
It was observed that there was a strong fluorescence quenching effect of
hesperidin or
icariin on
lysozyme . -
pubmed
The quenching constants of the drugs with
lysozyme were measured at different temperatures , and the quenching mechanism was suggested as dynamic quenching for
HES - LYS system and both static and dynamic quenching for
ICA - LYS system . -
pubmed
The thermodynamic parameters of the interaction of
hesperidin or
icariin and
lysozyme were measured according to the Van 's Hoff equation : the enthalpy change ( DeltaH ) and the entropy change ( DeltaS ) of HES - LYS system and
ICA - LYS system were calculated to be 20.29 kJ x mol(-1 ) and 146.28 J x mol(-1 ) x K(-1 ) , and -3.47 kJ x mol(-1 ) and 81.16 J x mol(-1 ) x K(-1 ) , respectively , which indicated that the interaction of
hesperidin and
lysozyme was driven mainly by hydrophobic force , whereas the interaction of
icariin and
lysozyme was driven mainly by electrostatic force . -
pubmed
The binding distances of
hesperidin and
icariin from the
lysozyme tryptophan residue were calculated to be 1.34 nm and 1.24 nm , respectively , based on the Forster 's theory of non - radiation energy transfer . -
pubmed
The results of synchronous fluorescence spectra showed that the binding of
hesperidin or
icariin to
lysozyme induced conformational changes in
lysozyme . -
pubmed
Sizing up
ethanol - induced plasticity : the role of
small and large conductance calcium - activated potassium channels . -
pubmed
Small ( SK ) and large conductance ( BK ) Ca ( 2 +)- activated K (+ ) channels contribute to action potential repolarization , shape dendritic
Ca ( 2 +)spikes and postsynaptic responses , modulate the release of hormones and neurotransmitters , and contribute to hippocampal - dependent synaptic plasticity . -
pubmed
Over the last decade ,
SK and BK channels have emerged as important targets for the development of acute
ethanol tolerance and for altering neuronal excitability following chronic
ethanol consumption . -
pubmed
In this mini - review , we discuss new evidence implicating
SK and BK channels in
ethanol tolerance and
ethanol - associated homeostatic plasticity . -
pubmed
Findings from recent reports demonstrate that chronic
ethanol produces a reduction in the function of SK channels in VTA dopaminergic and
CA1 pyramidal neurons . -
pubmed
There is also increasing evidence supporting the idea that
ethanol sensitivity of native BK channel results from differences in
BK subunit composition , the proteolipid microenvironment , and molecular determinants of the channel - forming subunit itself . -
pubmed
Taken together , these studies suggest that
SK and BK channels contribute to
ethanol tolerance and adaptive plasticity . -
pubmed
OBJECTIVES : This study sought to evaluate the frequency and efficacy of combination of
bivalirudin and provisional
glycoprotein ( GP ) IIb / IIIa blockade compared with
bivalirudin monotherapy in current clinical practice of percutaneous coronary intervention ( PCI ) with drug - eluting stents (
DES ) . -
pubmed
BACKGROUND : Previous randomized trials have demonstrated that a strategy of
bivalirudin with provisional ( bailout )
GP IIb / IIIa inhibition was non - inferior to
unfractionated heparin ( UFH ) plus planned
GP IIb / IIIa blockade for the prevention of acute and long - term adverse clinical events . -
pubmed
However , the frequency and efficacy of provisional
GP IIb / IIIa inhibition in addition to the full - dose
bivalirudin in current practice is not well established . -
pubmed
The incidence of in - hospital mortality ( 0 % vs. 0.3 % p = 1.000 ) ,
MI ( 7.1 % vs. 6.6 % ; p = 0.864 ) , and the combined endpoint of
death ,
stroke , emergent coronary artery bypass graft surgery ( CABG)/PCI , or
MI ( 7.1 % vs. 6.9 % ; p = 0.868 ) were similar in the
bivalirudin - plus - bailout
GP IIb / IIIa inhibitor versus the
bivalirudin - alone arm . -
pubmed
There was a higher incidence of
bleeding complications ( 16.0 % vs. 9.6 % ; p = 0.018 ) in the
bivalirudin - plus - bailout
GP IIb / IIIa versus the
bivalirudin - alone group . -
pubmed
At follow up , there were 4 ( 2.6 % )
deaths in the
bivalirudin - plus -
GP IIb / IIIa inhibitor group versus 83 ( 7.0 % )
deaths in the
bivalirudin - alone arm ( HR 0.36 , 95 % confidence interval [ CI ] 0.13 - 0.98 ; p = 0.044 ) . -
pubmed
After multivariate Cox regression analysis , bailout
GP IIb / IIIa use in addition to
bivalirudin was associated with similar long - term survival when compared to
bivalirudin monotherapy ( HR 0.41 , 95 % CI 0.15 - 1.12 ; p = 0.081 ) . -
pubmed
Efficacy of the treatment was assessed by measuring the histopathologic
injury score (
HIS ) , and biochemically by determining tissue
superoxide dismutase (
SOD ) ,
glutathione peroxidase (
GSH - Px ) , and
malondialdehyde (
MDA ) in ileum , liver and kidney homogenates . -
pubmed
Furthermore , we found that the C57BL/6J - like changes in
anxiety - related behavior after restraint were absent in null mutants lacking the modulatory
NMDA receptor subunit
Grin2a , but not the
AMPA receptor subunit
Gria1 . -
pubmed
The rate of hydrolysis of GXG with TXy and
accellerase was increased by 15 - 20 % in the presence of chemical surfactants (
tween 80 and
toluene ) or protein additive ( BSA ) . -
pubmed
The fermentation of enzymatic hydrolysates of GXG by TXy and
accellerase with free cells at 40 degrees C produced an
ethanol yield of 0.39 and 0.41 g / g whereas with immobilized cells produced 0.45 and 0.43 g / g , respectively , with a theoretical conversion efficiencies of 78 - 88 % . -
pubmed
RESULTS : Lower
glucose infusion rate was significantly associated with lower levels of
HDL cholesterol in youths with
Type 1 diabetes and with higher levels of
triglycerides and higher
triglyceride / HDL ratio in both youths and adults . -
pubmed
The
phenolic compounds both in
ethanol and water extracts of the plant were analysed using liquid chromatography - mass spectrometry ( LC -
MS ) and capillary zone electrophoresis . -
pubmed
The contents of the pharmaceutically important antidepressants
hyperforin and
hypericin were also estimated by LC -
MS and compared with the data in the literature . -
pubmed
After high - performance liquid chromatography analysis , we have 69.3 + -3.2 % of
[ ( 18 ) F ] F (- ) incorporation ratio on the manual synthesis and applied these conditions to automatic preparation with
GE TracerLab
FX module . -
pubmed
The pS422 antibody was used as an early
tau pathology marker that labels
tau phosphorylated at
Ser422 ; the
TauC3 antibody was used to detect later stage tau pathology . -
pubmed
Methylation status of
CpG sites in the MCP-1 promoter is correlated to serum
MCP-1 in
Type 2 diabetes . -
pubmed
Bodymass index , blood pressure , blood lipid , blood
glucose ,
glycosylated hemoglobin (
HbA1c ) , and serum
MCP-1 were measured . -
pubmed
Methylation status of
CpG sites in
theMCP-1 promoter was determined using methylation specific
polymerase chain reaction . -
pubmed
MCP-1 promoter methylation was significantly correlated to serum
MCP-1 ,
HbA1c , fasting blood
glucose , and
triglyceride . -
pubmed
CONCLUSION : These data suggest that hypomethylation of
CpG sites in the MCP-1 promoter region may be affected by blood
glucose and
TG , which then increase the serum
MCP-1 level and may play a role in the vascular complications of
Type 2 diabetes . -
pubmed
The primary end point was to assess differences in postoperative serum
alanine ,
aspartate aminotransferase (
ALT and
AST ) and
bilirubin levels . -
pubmed
The curves of serum
AST ,
ALT and
bilirubin levels in a span of time of five postoperative days were not significantly different between the three groups . -
pubmed
The FBs were incubated with different concentrations of
PDGF - AA and the changes in their proliferative activity were quantified with
MTT assay . -
pubmed
A novel series of
pyrazolylpiperidine N - type
calcium channel blockers . -
pubmed
A novel series of
pyrazole - based
N - type calcium channel blockers was identified by structural modification of a high - throughput screening hit and further optimized to improve potency and metabolic stability . -
pubmed
Die - off ratio correlates with increased
TNF - alpha :
IL-10 ratio and decreased
IVF success rates correctable with
humira . -
pubmed
However , mutating putative
GR binding sites did not substantially reduce induction of
ras -
dva promoter activity by
corticosterone , suggesting additional DNA elements within the 5'-flanking region are responsible for glucocorticoid regulation . -
pubmed
Decreased numbers of apoptotic type
II pneumocytes and mesenchymal cells , and decreased contents of proapoptotic
cleaved caspase-3 and - 7 and cytoplasmic
cytochrome c , and an increase in antiapoptotic
Bcl - xL were found in lungs treated with
diphenyl phenyl diamine . -
pubmed
The study was aimed to investigate the effect of
steroid substitution on serum
osteoprotegerin and
receptor activator of nuclear factor kappa - beta ligand (
RANKL ) levels in relation to bone mineral density ( BMD ) in
PAI . -
pubmed
Serum
osteoprotegerin increased with age ( p<0.001 ) , but showed no correlation with daily
hydrocortisone dose . -
pubmed
Osteoprotegerin was negatively correlated with serum
dehydroepiandrosterone - sulfate ( p=0.008 ) and with
BMD at the lumbar spine ( p<0.001 ) and femoral neck ( p=0.003 ) . -
pubmed
RANKL correlated negatively with PAI duration ( p=0.029 ) and positively with daily
hydrocortisone dose ( p=0.018 ) . -
pubmed
In conclusion , increased
osteoprotegerin in
PAI might reflect a compensatory response to enhanced
bone resorption due to exogenous
steroid excess and/or result from a deficit in adrenal
androgens . -
pubmed
RANKL levels remain within normal range on standard
steroid replacement . -
pubmed
Perianth explants in a relatively early stage of development were competent for differentiation of tepals on Murashige and Skoog (
MS ) medium supplemented with 2 mg 1(-1 )
N ( 6 )- benzylaminopurine (
BAP ) or
zeatin and 0.1 mg 1(-1 )
2,4 - dichlorophenoxyacetic acid ( 2,4 - D ) . -
pubmed
Alkaline phosphatase (
AP ) catalyses the detachment of
phosphate residues from different substrates . -
pubmed
The effect of different doses of AP ( 1.2 and 2.5 IU / mL ) on several sperm parameters after IVC ( viability , acrosome integrity with
FITC -
PSA , capacitation status with
CTC staining ,
tyrosine phosphorylation ) and on fertilizing ability during
IVF were also evaluated . -
pubmed
Apoptosis was evaluated with
annexin - V and
propidium iodide staining and flow - cytometry . -
pubmed
Ligand tuning of single - site
manganese - based catalytic antioxidants with dual
superoxide dismutase and
catalase activity . -
pubmed
A bio - inspired
manganese ( ii ) complex with a linear pentadentate ligand framework containing soft
sulfur donors and an alternating
NSNSN binding motif displays excellent dual
CAT /
SOD - like antioxidant activity with high turnover efficiency and good operation stability in an aqueous environment . -
pubmed
A nanowire - directed templating synthesis of metal - organic framework (
MOF ) nanofibers has been demonstrated , where ultrathin
tellurium nanowires ( TeNWs ) with excellent dispersivity can act as templates for directed growth and assembly of
ZIF-8 nanocrystals ( one typical
MOF ) , resulting in the formation of uniform
ZIF-8 nanofibers . -
pubmed
These terminal
oxidases have varying properties such as their affinity for
oxygen , transcriptional regulation and proton pumping ability . -
pubmed
In comparison with other
oxidase mutants , the
cyo mutant had a significantly longer lag phase under low -
oxygen conditions . -
pubmed
Using a
cyo : :
lacZ transcriptional fusion , it was shown that
cyo expression in the wild type peaks between 1 and 2.5 %
O2 . -
pubmed
In addition , it was shown with quantitative
reverse transcriptase PCR that
cyoB is upregulated approximately fivefold in 1 %
O2 compared with fully aerobic ( 21 %
O2 ) conditions . -
pubmed
Although it is commonly thought that
Cyo is utilized only at higher
oxygen concentrations , the results from this study indicate that
Cyo is important for adaptation to and sustained growth under low
oxygen . -
pubmed
Their stimulation by specific
testosterone albumin conjugates (
TAC ) activate rapidly non - genomic
FAK /
PI3K /
Rac1 /
Cdc42 signaling , trigger
actin reorganization and inhibit cell motility . -
pubmed
In line with this ,
TAC and
EMD638683 activated
caspase-3 . -
pubmed
These effects were insensitive to the classical
androgen receptor (
iAR ) antagonist
flutamide , pointing to
iAR - independent ,
mAR - induced responses . -
pubmed
Blocking
actin restructuring with
cytochalasin B abrogated the
TAC- and
EMD638683 - induced pro - apoptotic responses . -
pubmed
Our aims were , to clarify the blood lipid differences [ Total serum
cholesterol (
TC ) ,
High - density lipoprotein (
HDL ) ,
Low density lipoprotein (
LDL ) ,
Triglyceride (
TG ) ] between female patients who had attempted suicide and controls and to determine whether we could use the patients initial lipid profiles to predict suicide re - attempt within the subsequent year . -
pubmed
Blood
lactate rose from
DIA1 to DP to
DIA2 . -
pubmed
O2 extraction was attenuated during
DP ( P & lt ; 0.05 ) , but was the same during
DIA1 and
DIA2 . -
pubmed
The ratio of upper-/whole - body EMGRMS correlated negatively with
O2 extraction in the arms during both sessions of
DIA ( P & lt ; 0.05 ) . -
pubmed
These three new criteria are : greater than 60 % clonal plasma cells on bone marrow biopsy , a serum free light chain (
sFLC ) ratio of & gt;100 ( the involved sFLC must be & gt;100 mg / l ) and greater than one unequivocal focal lesion on advanced imaging ( low dose whole body computerized tomography , magnetic resonance imaging ,
( 18 ) F fluorodeoxyglucose positron emission tomography ) . -
pubmed
Before and after treatment , serum
estradiol (
E2 ) ,
testosterone ( T ) ,
luteinizing hormone (
LH ) ,
prolactin (
PRL ) by radioimmunoassay and
immunoglobulin (
IgG ) by immunodiffusion , erythrocyte sedimentation rate ( ESR ) by Westergren ,
interferon - gamma (
IFN - gamma ) and
interleukin-4 (
IL-4 ) by enzyme linked immunosorbent assay were determined . -
pubmed
GalN caused more severe
injury to steatotic hepatocytes than to lean cells as documented by
lactate dehydrogenase leakage . -
pubmed
Necrotic mode of cell
death strongly prevails over apoptosis since we did not observe any significant increase in activities of
caspase 3 , 8 and 9 in any group of hepatocytes treated with
GalN. -
pubmed
Impact Study :
MK-0646 ( Dalotuzumab ) ,
Insulin Growth Factor 1 Receptor Antibody Combined with
Pemetrexed and
Cisplatin in Stage
IV Metastatic
Non - squamous Lung Cancer . -
pubmed
Dalotuzumab -
MK0646 ( D ) is a humanized monoclonal antibody that targets
IGF-1R. -
pubmed
Serum
glucose ,
insulin and
amyloid A were measured 24 h before operation and at endpoints . -
pubmed
Bodyweight was increased by HFD , whereas serum
glucose ,
amyloid A and
insulin were not influenced . -
pubmed
TAT Peptide - Conjugated Magnetic
PLA -
PEG Nanocapsules for the Targeted Delivery of
Paclitaxel : In Vitro and Cell Studies . -
pubmed
The conjugation of a
cysteine - modified
TAT peptide (
HCys - Tyr - Gly - Arg - Lys - Lys - Arg - Arg - Gln - Arg - Arg - Arg - NH2 ) on the surface of the nanocapsules resulted to highly increased uptake of nanocapsules by
cancer cells , as well as to profound improvement of their
cytotoxicity against the
cancer cells . -
pubmed
In this study we assessed the
pain score - VAS at
rest at 2 , 4 , 6 , 12 and 24 hours after the operation and the total analgesic consumption of
morphine over 24 hours . -
pubmed
The cumulative 24 hours
morphine consumption after the operation was significantly lower in group III ( 5.53 1.21 mg ) than in group
II ( 6.16 2.41 mg ) and group I ( 9.26 2.41 mg ) . -
pubmed
CONCLUSION : Concerning the
inguinal hernia repair we found better postoperative
pain scores and 24 hours reduction of the
morphine consumption in group III (
GA and
TAP - D block ) compared with group I (
GA ) and group
II (
GA +
TAP block ) . -
pubmed
The
claudin-4 mediated
chloride permeability can be regulated by a
protease endogenously expressed by the
collecting duct cell - channel - activating protease 1 . -
pubmed
Deletion of either
claudin protein from the collecting duct causes renal
chloride reabsorption defects and
low blood pressure . -
pubmed
Corneal edema was significantly less and the increases in anti - oxidative
HO-1 ,
CAT and
SOD2 mRNA expressions were significantly suppressed in the
H2 group . -
pubmed
In addition , corneal endothelial cell expressions of two oxidative stress markers , 4 -
HNE and
8 - OHdG , were significantly lower in the
H2 group . -
pubmed
We found that
macrophage colony - stimulating factor - dependent macrophage differentiation and subsequent
receptor activator of NF - kappaB ligand - induced osteoclast formation were largely suppressed by adding
TAS-115 . -
pubmed
The phosphorylation of the
macrophage colony - stimulating factor receptor FMS and
osteoclast related kinases such as
ERK and
Akt were also suppressed by the presence of
TAS-115 . -
pubmed
RESULTS :
Naftopidil treatment reduced the upregulation of mRNA levels of
ischemia and
fibrosis markers at the early phase of
SCI , and ameliorated the decrease of bladder compliance and voiding efficiency , and the increase of urethral pressure and
collagen concentration in the bladder wall at the late phase of
SCI . -
pubmed
Tadalafil treatment reduced the upregulation of mRNA levels of
fibrosis markers , the decrease of bladder compliance and the increase of
collagen concentration at the late phase of
SCI . -
pubmed
CONCLUSIONS : These results suggest that
naftopidil and
tadalafil treatments improved the bladder remodeling shown by increased bladder
collagen contents after
SCI in a different time course . -
pubmed
BACKGROUND :
Celecoxib is a selective
cyclo - oxygenase-2 inhibitor recommended orally to treat
arthritis and
osteoarthritis . -
pubmed
OBJECTIVES : We tested the hypothesis that
nicotine acting at
nicotinic acetylcholine receptors (
nAChRs ) would augment Pavlovian
alcohol - seeking . -
pubmed
RESULTS : All paired groups acquired conditioned port - entry responding to the
CS , indicative of Pavlovian
alcohol - seeking , whereas unpaired control group did not . -
pubmed
Pre - session
nicotine injections increased
CS port - entries relative to saline , only in the paired group . -
pubmed
This
nicotine - induced enhancement of Pavlovian
alcohol - seeking was blocked by pre - treatment with the
nAChR antagonist
mecamylamine . -
pubmed
Photobiosynthesis of stable and functional
silver /
silver chloride nanoparticles with hydrolytic activity using hyperthermophilic
beta - glucosidases with industrial potential . -
pubmed
The syntheses were straightforward and rapid processes carried out by mixing
beta - glucosidase and
silver nitrate ( in buffer 10mM
Tris -
HCl , pH 8) under irradiation with light ( over a wavelength range of 450 - 600 nm ) , therefore , compatible with the green chemistry procedure . -
pubmed
Finally , the results showed that the enzymatic activities were maintained in the
beta - glucosidases assisted
Ag /
AgCl - NPs . -
pubmed
Although the
pilocarpine - induced
SE itself is not significantly reduced , the
SE - induced neuronal cell
death is significantly reduced in the
CA1 and the lateral septum , indicating that
TRPC1 / 4 channels directly contribute to
SE - induced neuronal cell
death . -
pubmed
The use of LABA+
LAMA association without ICS increased from 0.9 to 7 % , but remained lower than the fixed LABA+ICS association ( 26 % ) , less often prescribed than the triple association LABA+ICS+LAMA ( 32.5 % in
period 2 ) . -
pubmed
AIM : The aim of this study is to identify the potential role of high protein - low
carbohydrate ketogenic diet ( KD ) in managing
weight loss in patients who underwent gastric banding and did n't lose weight anymore . -
pubmed
RESULTS : An in vitro assay for the glycation of
bovine serum albumin by
methylglyoxal showed that
GS - E3D inhibited AGE formation at an IC50 value of 19.65 + - 4.35 mug / mL. -
pubmed
A novel
ferrocene - based substrate for the ratiometric electrochemical detection of
beta - galactosidase was designed and synthesised . -
pubmed
Herein , well - defined hollow dendritic
AuPtAg alloyed nanocrystals ( ANCs ) were synthesized by a simple
L - proline - mediated one -
pot aqueous method . -
pubmed
The resultant label - free immunosensor exhibited the improved performance for highly sensitive detection of
PSA based on the enhanced catalytic currents of
K3 [ Fe ( CN ) 6 ] as a signal probe . -
pubmed
An increase in
auxin level in the root tips was associated with increased expression of auxin biosynthesis genes and auxin polar transporter
AUX1 and
PIN2 under BA stress . -
pubmed
Furthermore , BA stimulated
ethylene evolution , whereas blocking BA - induced
ethylene signaling with
ethylene biosynthesis inhibitor (
Co2 + ) ,
ethylene perception antagonist ( 1 -
MCP ) or
ethylene signaling mutant lines
etr1 - 3 and
ein3eil1 compromised BA - mediated inhibition of root elongation , up - regulation of
auxin biosynthesis - related genes and
AUX1 and
PIN2 , indicating that
ethylene signal was involved in
auxin - mediated inhibition of primary root elongation during BA stress . -
pubmed
Taken together , our results reveal that allelochemical BA inhibits root elongation by increasing
auxin accumulation by stimulating
auxin biosynthesis and
AUX1 /
PIN2 - mediated
auxin transport via stimulating
ethylene production and an
auxin /
ethylene independent ROS burst . -
pubmed
The EOs was also found to be effective in reducing virulence factors like
staphyloxanthin and
hemolysin . -
pubmed
The results suggest that
( E )- nerolidol showed better binding affinity towards the
enzyme . -
pubmed
Correction : Synergistic Interaction between the
HDAC Inhibitor ,
MPT0E028 , and
Sorafenib in
Liver Cancer Cells In Vitro and In Vivo . -
pubmed
RATIONALE : High - precision determination of
magnesium (
Mg ) isotopes can now be routinely achieved by multi - collector inductively coupled plasma mass spectrometry ( MC - ICP -
MS ) . -
pubmed
Determination of elevated levels of
nitrate in vegetable powders by high - precision isotope dilution
GC -
MS . -
pubmed
A high - precision isotope dilution
GC -
MS method was employed for the determination of
nitrate in processed vegetables . -
pubmed
The samples were extracted in water , derivatized with
triethyloxonium tetrafluoroborate and analyzed by headspace
GC -
MS ( 15 samples / h ) . -
pubmed
We then describe our efforts to apply site - specific 1D and 2D IR spectroscopy toward elucidation of protein conformations and dynamics to investigate their involvement in protein molecular recognition , in particular mediated by dynamic complexes :
plastocyanin and its binding partner
cytochrome f ,
cytochrome P450s and substrates or redox partners , and
Src homology 3 domains and
proline - rich peptide motifs . -
pubmed
However , the few prior studies exclusively relied on
spot measurements of phasic
HPA - axis activity , which may not adequately capture
cortisol output over prolonged periods of time . -
pubmed
CONCLUSION : In conjunction with data from previous studies , it is thus tempting to speculate that
sGC may rather affect the capacity of dynamic changes and flexible adaption of an individual 's
HPA - axis than changes in tonic
steroid output . -
pubmed
The
TS stabilization of up to ~10 kcal / mol was consistent with the formation of a strong n pi * stabilization between the
imide carbonyl oxygens and the ortho R group in the planar TS . -
pubmed
beta - Etherases are enzymes with a promising potential for application in lignin depolymerization due to their selectivity in the cleavage of
beta - O-4 aryl ether bonds . -
pubmed
TMAO levels after 6 months of
l - carnitine supplementation were associated with higher
low - density lipoprotein -
cholesterol (
LDL - c ) ( Spearman Rho = 0.518 , p = 0.003 ) and total
cholesterol (
TC ) ( Spearman Rho = 0.407 , p = 0.026 ) levels . -
pubmed
D - loop methylation was not directly correlated to the
TMAO augmentation observed in the supplemented group , but its increase inversely correlated with
TC ( Pearson coefficient = -0.529 , p = 0.029 ) and
LDL - c ( Pearson coefficient = -0.439 , p = 0.048 ) . -
pubmed
Overall , no significant difference in mortality was observed between the
TP and
catecholamine groups ( risk ratio(RR ) , 0.85 ( 0.70 to 1.03 ) ; P = 0.09 ) . -
pubmed
DEGs were mainly enrich in '
sterol biosynthetic and metabolic process ' , '
cholesterol metabolic and biosynthetic process ' by GO enrichment analysis . -
pubmed
The effects of high - intensity ultrasound ( HIU ) treatment ( 20 kHz at 400 W , 0 , 5 , 20 , and 40 min ) on the physical , structural , and aggregation properties of
DGH were investigated with the aim to reveal the influence of the selectively hydrolyzing glycinin component on the
HIU treatment of soy protein . -
pubmed
Our objective was to assess the effect of
nitric oxide added to the sweep gas of the oxygenator during cardiopulmonary bypass (
CPB ) in infants on platelet count , platelet function , clinical outcomes , and safety . -
pubmed
Nitric oxide added to the sweep gas of the oxygenator during
CPB in infants did not have an appreciable effect on preservation of platelet count , platelet responsiveness to agonist , or clinical outcomes . -
pubmed
In contrary ,
caveolin-1 depletion shows no effect on the expression , phosphorylation ( on sites
Ser39 ,
Ser56 , and
Ser83 ) , distribution , solubility , and cellular dynamics of
vimentin filaments . -
pubmed
Uterine
PGR responds to the heightened levels of ovarian
progesterone ( P4 ) after ovulation and regulates uterine gene transcription for successful embryo implantation . -
pubmed
This study aimed to synthesize and characterize
L - epigallocatechin gallate (
EGCG ) complexed
Mn2 + nanoparticle ( L - EGCG -
Mn ) , a proof - of - concept pH - sensitive
manganese core nanoparticle (
NP ) , and compare its magnetic resonance ( MR ) properties with those of
Gd - DTPA , both in vitro and in vivo . -
pubmed
It was suggested that the inhibition of
prostaglandin synthesis by
flurbiprofen might be due to the inhibition of the
endoperoxygenase which catalyzed conversion of
arachidonic acid to
cyclic endoperoxide . -
pubmed
In contrast ,
phenylbutazone which was a
pyrozolone derivative inhibited the formation of
prostaglandin E2 , but not affected the
endoperoxygenase reaction . -
pubmed
The kinetic studies for inhibition of
prostaglandin E2 synthetase indicated that
flurbiprofen competitively inhibited
prostaglandin E2 synthesis , just like
indomethacin . -
pubmed
Cytochalasin B , which inhibits phagocytosis , acts by interfering with the interaction between
actin - binding protein and
actin . -
pubmed
Conversely both
hydrazono compounds decreased the
monoamine oxidase activity much less , than
phenelzine . -
pubmed
CHEHP ( 145 mumol / kg ) inhibited this
enzyme by less than 14 % . -
pubmed
Plasma renin activity ( PRA ) and
PRA responsiveness to
sodium depletion were normal , suggesting that extracellular fluid volume was not increased . -
pubmed
Relationships between
plasma renin activity and urinary and plasma
catecholamines . -
pubmed
Effect of ergot drugs on central
5 - hydroxytryptamine neurons : evidence for
5 - hydroxytryptamine release or
5 - hydroxytryptamine receptor stimulation . -
pubmed
It was found that
ergocornine increased the
5 - HT receptor activity independent of presynaptic
5 - HT stores and that it didnot have any effects on uptake , retention and spontaneous overflow of
3 - H-5 - HT in vitro but reduced the fiedl stimulation - induced release of
3 - H-5 - HT in vitro . -
pubmed
Therefore , it is suggested that
ergocornine is a
5 - HT recpetor - stimulating agent , an effect which may lead to reduction of nervous impulse flow in the 5 -
HT neurons and subsequently of
5 - HT release and turnover . -
pubmed
MPME , on the other hand , seems to increase
5 - HT receptor release of
5 - HT stores , mainly from extragranular sites . -
pubmed
Inhibition of the
5 - HT membrane pump does not seem to be of any major importance , since
chlorimipramine was only weakly active on the extensor reflex in the pharmacological models used and since
MPME did not block but rather enhanced the
5 - HT depletion caused by
4 - methyl - alpha - ethyl - m - tyramine . -
pubmed
It is suggested that
MPME is a releaser of extragranular
5 - HT stores leading to increased
5 - HT receptor activity and reduction of
5 - HT turnover in the same way as indicated for
ergocornine . -
pubmed
In spite of the fact that in
carbon - limited chemostat cultures under induced conditions the
enzymes involved in
methanol metabolism were present at wild - type ( WT ) levels , this mutant is unable to grow on
methanol as a sole
carbon and energy source . -
pubmed
Addition of
methanol to
glucose - limited ( SR = 12.5 mM ) chemostat cultures of the
PER mutant only resulted in an increase in yield when small amounts were used ( up to 22.5 mM ) . -
pubmed
The
enzyme patterns obtained suggested that the ultimate decrease in yield observed at enhanced
methanol concentrations was due to an inefficient
methanol - metabolism as a consequence of the absence of peroxisomes . -
pubmed
The absence of intact peroxisomes results in two major problems namely i ) in
H2O2 - metabolism , which most probably is no longer mediated by
catalase and ii ) the inability of the cell to control the fluxes of
formaldehyde , generated from
methanol . -
pubmed
Nicotinic acid and its derivatives block adipose tissue lipolysis thus reducing the
FFA flux of free
fatty acids to the liver and the synthesis of
very - low - density lipoproteins (
VLDL ) . -
pubmed
Clofibrate and its derivatives decrease the synthesis of
VLDL and increase their catabolism . -
pubmed
Lipoprotein lipase activity as well as
VLDL fractional catabolic rate are increased by
fibrates . -
pubmed
On the mechanism of
rotenone - insensitive reduction of
quinones by mitochondrial
NADH : ubiquinone reductase . -
pubmed
NADH acts as an incomplete competitive inhibitor for
5,8 - dioxy-1,4 - naphtoquinone during its
rotenone - insensitive reduction by mitochondrial
NADH : ubiquinone reductase . -
pubmed
The bimolecular rate constant of the reduction of the
enzyme by
NADH in the
quinone reductase reaction is about 2 times less than that of
ferricyanide reductase reaction . -
pubmed
It seems that during the steady - state reduction of
ferricyanide and
5,8 - dioxy-1,4 - naphtoquinone these oxidizers react with
NADH : ubiquinone reductase reduced to different extents . -
pubmed
Interaction of
1,25 - dihydroxyvitamin D and
plasma renin activity in high
renin essential
hypertension . -
pubmed
Renin secretion by the kidney is inhibited by an increase in free intracellular
calcium concentration . -
pubmed
An inverse correlation existed between the change in
vitamin D and the change in
plasma renin activity ( r = -0.765 , P = .01 ) . -
pubmed
It is postulated that the increase in dietary
sodium led to an increase in serum
1,25 - dihydroxyvitamin D concentration , which may have contributed to an increase in intracellular
calcium concentration , a decrease in renal secretion of
renin , and a fall in
plasma renin activity . -
pubmed
Therefore ,
1,25 - dihydroxyvitamin D may be a mediator in the response of high
renin hypertension to increased
sodium intake . -
pubmed
One of the genes , apparently by means of alternate splicing , gives rise to two
prothymosin alpha mRNAs , one of which has an additional internal
glutamic acid codon with respect to the other . -
pubmed
It was demonstrated on short columns packed with these anion exchangers that ( i ) a substantially higher resolution of
proteins and
nucleotides was obtained at gradient times of less than 5 min than on porous anion exchangers ; ( ii ) the low external surface area of the non - porous anion exchanger is not a critical parameter in analytical separations and ( iii ) microgram - amounts of
enzymes of high purity and full biological activity were isolated . -
pubmed
Preparations of monoclonal antibodies directed against some blood group
antigens and
anti - A1 lectin reagent were devoid of
spectrin autoantibody as expected . -
pubmed
Care must be exercised in studies designed to determine the association of some blood group
antigens with the red cell membrane skeleton , when using polyclonal sera which contain
spectrin autoantibodies in addition to the antibodies against the blood group
antigen in question . -
pubmed
Recently
Cyclosporin A has been shown to cause
renal damage , which correlates with increased glomerular
neutrophil migration . -
pubmed
Lower peptides and
amino acids in hydrolyzates of
casein obtained with protosubtilin were determined using high pressure liquid chromatography ( HPLC ) . -
pubmed
Threshold of
nickel sensitivity and
immunoglobulin E determinations . -
pubmed
hCRF caused brief
pulse - like elevations of plasma
IR - ACTH and
cortisol without any effect on the plasma concentrations of
GH ,
PRL , and
LH . -
pubmed
This is in contrast to
oCRF , which causes prolonged
ACTH and
cortisol secretion . -
pubmed
The oxidation of
ferrocytochrome c mediated by
cytochrome c oxidase was investigated in the presence of ferricytochrome c ,
trifluoroacetyl -
cytochrome c , the
heme fragments
Hse65 -[ 1 - 65 ] and
Hse80-[1 - 80 ] and their respective
porphyrin derivatives , as well as carboxymethylated
apoprotein and related fragments , polycations , salts and neutral additives . -
pubmed
The
protein content of insect venoms was determined using a
Coomassie blue dye binding assay as well as the Lowry
phenol assay . -
pubmed
Basal plasma
insulin before pregnancy was elevated and there was no rise from
glucose ; after parturition the basal and pattern of response was normal . -
pubmed
Correlation between effects of 24 different
cytochalasins on cellular structures and cellular events and those on
actin in vitro . -
pubmed
The following effects were tested for each of 24
cytochalasins ; ( a ) four high dose ( 2 - 20 muM ) effects on the cellular level : rounding up of fibroblastic cells , contraction of
actin cables , formation of hairy filaments containing
actin , and inhibition of lymphocyte capping ; ( b ) a low dose ( 0.2 - 2 muM ) effect : inhibition of membrane ruffling ; and ( c ) two in vitro effects : an inhibition of
actin filament elongation ( the high affinity effect [ low dose effect ] in vitro ) and an effect on viscosity of
actin filaments(the low affinity effect [ high dose effect ] in vitro ) . -
pubmed
From the data obtained with the 24
cytochalasins , we have calculated correlation coefficients of 0.87 and 0.79 between an effect in vivo , inhibition of capping , and an effect in vitro , inhibition of
actin filament elongation , as well as between inhibition of capping and another effect in vitro , effect on viscosity of
actin filaments , respectively . -
pubmed
The strong positive correlation among low and high dose effects in vivo and those in vitro suggests that most of the effects caused by a
cytochalasin , irrespective of doses or affected phenomena , might be attributed to the interaction between the drug and the common target protein ,
actin . -
pubmed
In control cells with functional classic
cadherins , we observed redistribution of
desmoplakin to cell - cell borders with insertions of
keratin filaments at the contact sites as soon as 2 h after
calcium elevation , after an earlier event of
E - cadherin translocation to the cell - cell contact sites . -
pubmed
These findings indicate that the disruption of
classic cadherin affected the organization of desmosomes upon
calcium elevation and suggest that the proper function of classic cadherins is a prerequisite for desmosome assembly in keratinocytes . -
pubmed
The effect of
ethyl-2 -[ 6 -( 4 - chlorophenoxy ) hexyl ) oxirane-2 - carboxylate (
etomoxir ) and its
oxirane analogues on the expression of several genes from liver and testis as well as the beneficial effect of
etomoxir on heart performance and
myosin isozyme expression is reviewed . -
pubmed
The simultaneous addition of
etomoxir and a fat diet produces an increase in the expression of
carnitine palmitoyl transferase ( CPT ) I ,
cytochrome P-450 4A1 omega - hydroxylase and
fatty acid binding protein (
L - FABP ) . -
pubmed
The mRNA levels of other genes such as
CPT II ,
3 - hydroxy-3 - methylglutaryl - CoA ( HMG - CoA ) reductase , and
fatty acid synthase (
FAS ) are increased by
etomoxir alone . -
pubmed
Neither cytosolic nor mitochondrial
HMG - CoA synthase have any significant effect on the mRNA levels induced by
etomoxir . -
pubmed
A probably frequent mechanism for the action of
etomoxir may involve the overload of non - metabolized
fatty acids produced after the inhibition of
CPT I by the
oxirane compounds . -
pubmed
There is some speculation as to whether the
peroxisome proliferator activated receptor (
PPAR ) increases its participation in the expression , under the action of
etomoxir . -
pubmed
The presence of numerous
nitric oxide synthase - positive neurons , all along the colon and at both plexuses , supports the hypothesis that an excessive production of
nitric oxide may cause the persistent inhibition of contractions . -
pubmed
The N - terminally truncated peptide ( 95 - 107 ) was also cleaved at that bond by both
proteinases , but far less rapidly than peptide 91 - 107 , and additional cleavages appeared at secondary sites i.e those generating
atriopeptin III ( R101 ) and
auriculin ( R102 ) in
rat pro - ANP . -
pubmed
Particular ability of liver
P450s3A to catalyze the oxidation of
N omega - hydroxyarginine to
citrulline and
nitrogen oxides and occurrence in no
synthases of a sequence very similar to the
heme - binding sequence in
P450s . -
pubmed
Specific inhibitors of
P450s3A as
troleandomycin and
dihydroergotamine strongly inhibit
NOHA oxidation whereas
metyrapone , an inhibitor of other
P450 subfamilies , was without effect . -
pubmed
These data show the particular ability of
P450s of the
3A subfamily to catalyze the second step of the oxidation of
L - arginine by
NO synthases (
NOS ) . -
pubmed
This analogy between
NOSs and
P450s3A is further substantiated by a protein sequence comparison which shows that a
9 - amino acid segment present in all
NOSs exhibits a strong similarity with the sequence mainly responsible for
heme binding in
P450s3A which is well conserved in all
P450s . -
pubmed
This segment contains all the structural factors which are thought to be crucial for
heme binding in
P450s . -
pubmed
[ 2 - 3H ] Glucosamine was prepared by
acid hydrolysis of the labelled
N - acetylglucosamine and was converted to
[ 2 - 3H ] glucosamine 6 - phosphate by incubation with
hexokinase and
ATP . -
pubmed
The
sugar phosphate was used as a substrate for
glucosamine 6 - phosphate deaminase (
isomerase ,
EC 5.3.1.10 ) in a simple
3H2O release assay . -
pubmed
Endothelin (
ET ) ,
atrial natriuretic peptide (
ANP ) ,
catecholamine (
epinephrine ,
norepinephrine ) plasma levels , and
ACE activity were measured from arterial blood sampled before injection of
enalaprilat or
NaCl solution ( baseline values ) , 10 min and 30 min thereafter , immediately before the start of cardiopulmonary bypass (
CPB ) , immediately after
CPB , and at the end of surgery . -
pubmed
Binding was also reduced by pretreatment with
trypsin and
papain , and by
8 - anilino-1 - naphthalenesulphonic acid , which binds to hydrophobic
amino acids . -
pubmed
Palladium-103 : a new radioactive source in the treatment of unresectable
carcinoma of the pancreas : a phase I -
II study . -
pubmed
After considering some of the advantages of Pd-103 over I-125 , we designed a phase I -
II clinical trial to assess the feasibility of intraoperative
Pd-103 in unresectable
carcinoma of the pancreas to study the related morbidity when combined with chemotherapy and external beam radiation , and to evaluate the impact on palliation and local control rates . -
pubmed
The mean number of
Pd-103 pellets was 45 ; the mean total activity to obtain a matched peripheral dose (
MPD ) of 11,000 cGy was 68.9 mCi . -
pubmed
Activity of
Parthenolide at
5HT2A receptors . -
pubmed
These results suggest that
parthenolide may be a low - affinity antagonist at
5HT receptors ; it is unlikely that the entire mechanism of action can be explained by its modest
5HT2A receptor affinity . -
pubmed
Our data suggest a model whereby local production of
phosphatidylinositol phosphates may trigger the binding of vesicles to the active zone via the
Mint . -
pubmed
From its periplasmic location ,
Cu ,
Zn SOD was inferred to acquire its substrate from outside the bacterial cell and was speculated to play a role in preserving meningococci from the action of microbicidal
oxygen free radicals produced in the context of host defense . -
pubmed
A
sodC mutant was constructed by allelic exchange and was used to investigate the role of
Cu ,
Zn SOD in pathogenicity . -
pubmed
The mutant showed no increased sensitivity to
paraquat , which generates
superoxide within the cytosol , but was approximately 1,000 - fold more sensitive to the toxicity of
superoxide generated in solution by the
xanthine / xanthine oxidase system . -
pubmed
We conclude that periplasmic
Cu ,
Zn SOD contributes to the virulence of
Neisseria meningitidis , most likely by reducing the effectiveness of toxic
oxygen host defenses . -
pubmed
METHODS : Qualitative morphological study on
Rb was performed by means of light microscope , electron microscope and TdT mediated
biotin - dUTP nick - end labeling ( TUNEL ) . -
pubmed
While
Panpal did not significantly influence the
acetylcholinesterase activity in brain following non - treated as well as treated soman
poisoning . -
pubmed
Panpal increased so many - induced
acetylcholinesterase inhibition following non - treated
poisoning and decreased the reactivating effect of the
oxime HI-6 following treated soman
poisoning in diaphragm . -
pubmed
CONCLUSIONS : Our data confirm the importance of the combination of reversible
acetylcholinesterase inhibitor
pyridostigmine with anticholinergic drugs in the pharmacological prophylaxis of soman
poisoning because of the elimination of consequences of
pyridostigmine - induced increasing in
acetylcholinesterase inhibition in the peripheral compartment . -
pubmed
The potency of the responses to other
nucleotides :
UTP & gt;=
ATP & gt ;
ADP & gt;> ;
AMP =
adenosine indicates the involvement of a mixture of
P2Y receptors . -
pubmed
Red blood cells of weak D phenotype have a much reduced number of presumably complete
D antigens that were repeatedly reported to carry the
amino acid sequence of the
regular RhD protein . -
pubmed
Likewise , in solid systems obtained by the solvent and melting solvent methods the drug solidifies in form III in
ethanol / water and
methanol while polymorph
IV crystallizes in
chloroform . -
pubmed
Vasoactive intestinal polypeptide (
VIP ) and
nitric oxide (
NO . ) -
pubmed
N ( omega )- nitro - L - arginine and
hemoglobin inhibited the rapid component but affected the sustained component less . -
pubmed
The
calcitonin gene - related peptide (
CGRP ) antagonist
CGRP -( 8 - 37 ) did not alter
EFS - induced LES relaxation . -
pubmed
A high positive charge is the common characteristic shared by the beta - sheet region of
stromal cell - derived factor-1 (
SDF-1 ) and
CXCR4 antagonists such as
ALX40 - 4C consisting of nine
D - arginines . -
pubmed
In the first study , peptide analogs possessing
amino acid sequences from both the N - terminus and the beta - sheet region of
SDF-1 were used as models to study the functional role of the beta - sheet region of
SDF-1 . -
pubmed
In the second study , two peptides containing nine
arginines and the N - terminal signal sequence of
SDF-1 were used as models to study the receptor binding mechanism of
CXCR4 antagonists of high positive charges such as ALX40 - 4C. -
pubmed
One peptide did not show signaling activity as indicated by the lack of
calcium influx while another peptide induced unusual
calcium influx distinct from that induced by the
SDF-1 N - terminal peptide . -
pubmed
Sp1 and
NF - Y synergistically mediate the effect of
vitamin D ( 3 ) in the
p27 ( Kip1 ) gene promoter that lacks
vitamin D response elements . -
pubmed
Consistent with the roles of these
transcription factors , treatment with
vitamin D ( 3 ) stimulated the DNA binding activities of these factors to each element and induced the change of one
NF - Y subunit . -
pubmed
Importantly , the
sMR has maintained its
mannose - binding properties since it was capable of binding a mannosylated ligand . -
pubmed
The high amount of
sMR released by DC and its ability to bind mannosylated ligand might indicate that this
molecule plays a role in the transport of mannosylated proteins from the site of
inflammation to other parts of the body . -
pubmed
Moreover , nicotinic survival support was not further enhanced by
fibroblast growth factor , as seen for
KCl , but increased to 100 % by adding
PACAP , a trophic neuropeptide present in the
ganglion . -
pubmed
Total
magnesium , ionized
magnesium ,
albumin , ionized
calcium ,
potassium , and pH did not change significantly by the administration of 1 g of
magnesium sulfate . -
pubmed
The cerebral blood flow was analyzed using
99mTc -
ECD - SPECT . -
pubmed
Applications of
nylon membrane arrays
to gene expression analysis . -
pubmed
Kinesin is a molecular motor that interacts with microtubules and uses the energy of
ATP hydrolysis to produce force and movement in cells . -
pubmed
From the polarization experiments with the bifunctional probe , we determined the orientation of
kinesin bound to microtubules in the presence of
AMP -
PNP and found close agreement with previous models derived from cryo - electron microscopy . -
pubmed
We also compared the polarization anisotropy of monomeric and dimeric
kinesin constructs bound to microtubules in the presence of
AMP -
PNP . -
pubmed
rHuIL-12 was generally well tolerated , but was associated with temporary decreases in neutrophils and lymphocyte counts , and with elevations in
serum transaminases and
bilirubin . -
pubmed
ERCC1 and
thymidylate synthase mRNA levels predict survival for
colorectal cancer patients receiving combination
oxaliplatin and
fluorouracil chemotherapy . -
pubmed
PURPOSE : To test the hypotheses of whether the relative mRNA expression of the
thymidylate synthase (
TS ) gene and the excision cross - complementing (
ERCC1 ) gene are associated with response to and survival of
fluorouracil (
5 - FU )/ oxaliplatin chemotherapy in metastatic
colorectal cancer . -
pubmed
CONCLUSION : These data suggest that intratumoral
ERCC1 mRNA and
TS mRNA expression levels are independent predictive markers of survival for
5 - FU and
oxaliplatin combination chemotherapy in
5 - FU - resistant metastatic
colorectal cancer . -
pubmed
Estrogen action via the
G protein - coupled receptor ,
GPR30 : stimulation of
adenylyl cyclase and
cAMP - mediated attenuation of the
epidermal growth factor receptor - to -
MAPK signaling axis . -
pubmed
Estrogen triggers rapid yet transient activation of the
MAPKs ,
extracellular signal - regulated kinase ( Erk )- 1 and
Erk-2 . -
pubmed
We have reported that this
estrogen action requires the
G protein - coupled receptor ,
GPR30 , and occurs via
Gbetagamma - subunit protein - dependent transactivation of the
epidermal growth factor ( EGF ) receptor through the release of pro - heparan - bound
EGF from the cell surface . -
pubmed
Here we investigate the mechanism by which
Erk-1 /- 2 activity is rapidly restored to basal levels after
estrogen stimulation . -
pubmed
Evidence is provided that attenuation of
Erk-1 /- 2 activity by
estrogen occurs via
GPR30 - dependent stimulation of
adenylyl cyclase and
cAMP - dependent signaling that results in
Raf-1 inactivation . -
pubmed
Moreover ,
GPR30 - dependent ,
cAMP - mediated attenuation of
EGF - induced
Erk-1 /- 2 activity was achieved by
ER antagonists such as
tamoxifen or
ICI 182 , 780 ; yet not by
17alpha - E2 or
progesterone . -
pubmed
Thus , our data delineate a novel mechanism , requiring
GPR30 and
estrogen , that acts to regulate
Erk-1 /- 2 activity via an inhibitory signal mediated by
cAMP . -
pubmed
Coupled with our prior findings , these current data imply that
estrogen balances
Erk-1 /- 2 activity through a single
GPCR via two distinct
G protein - dependent signaling pathways that have opposing effects on the
EGF receptor - to -
MAPK pathway . -
pubmed
Conversely , the
calcium - regulating receptors
P2X ( 1 - 3 ) and
P2Y ( 2 ) ( found in
normal but not
neoplastic skin ) were fully de - expressed within 2 microm of the
melanoma but fully expressed beyond that distance . -
pubmed
The effect of substituted
1,4 - anthraquinones on the photochemical activity and
chlorophyll fluorescence of
thylakoid membranes was examined . -
pubmed
Stronger
quinone - induced quenching of the
chlorophyll fluorescence than
quinone - induced changes in the activity of
photosystem II is observed . -
pubmed
The type (
Cl or
Br ) and the position (
Cl ) of the
chalogen atom strongly influence the degree of inhibition of
PSII electron transport and the quenching of
chlorophyll fluorescence . -
pubmed
The
collagen content and the ratio of collagenous to noncollagenous proteins (
hydroxyproline :
proline ratio ) were determined by performing
amino acid analysis on hydrolyzed samples using high - performance liquid chromatography . -
pubmed
Collagen denaturation was quantified with a colorimetric assay by determining the amount of
hydroxyproline released from samples treated with
alpha - chymotrypsine . -
pubmed
The
hydroxyproline :
proline ratio showed the same pattern as the
collagen content . -
pubmed
The percentage of
collagen denaturation in the superficial layer was always significantly ( P & lt ; 0.01 ) higher than that in the deep layer , but no effect of the
progesterone status or of the segment along the longitudinal axis was seen . -
pubmed
Consistent with this ,
zeo1 - deficient cells suppress the growth defect of mutants in the
Rho1p GDP -
GTP exchange factor
Rom2p , while exacerbating the growth defect of
sac7delta mutants at 37 degrees C. In contrast ,
mid2delta mutants have opposing effects to
zeo1delta mutants , being synthetically lethal with
rom2delta , and suppressing an 18 degrees C growth defect of
sac7delta , while overexpression of
MID2 rescues a rom2delta 37 degrees C growth defect . -
pubmed
[ Prolonged
insulin coma ; therapeutic effect of
ANP-235 ] . -
pubmed
coli is a
homodimer in solution with a specific activity of 3.8 U mg(-1 ) and K(m ) for
UDP - Gal of 114 microM. -
pubmed
cruzi
UDP - Glc 4 '- epimerase is unable to inter - convert
UDP -
N - acetylglucosamine and
UDP - N - acetylgalactosamine . -
pubmed
cruzi initiates O - glycosylation of its abundant
GPI - anchored surface mucins via
GlcNAcalpha1 - O - Thr /
Ser rather than the
GalNAcalpha1 - O - Thr /
Ser linkage that is common for
mucins from many other eukaryotes . -
pubmed
There was no convincing evidence of reaccumulation of
iron in the grafted liver in
HH ; however , 1 subject demonstrated increased serum
ferritin concentration and grade 2
hepatic siderosis . -
pubmed
The full - length cDNA consisted of a 1,561 - bp open reading frame encoding
lacIIIb , a 499 - amino - acid protein and a
21 - amino - acid signal peptide . -
pubmed
However , recent studies identify a distinct mode for
PKCdelta activation involving
tyrosine phosphorylation by
Src family kinases . -
pubmed
The
tyrosine - phosphorylated form of
PKCdelta ( which accumulates in the soluble fraction of cells exposed to oxidant stress ) displays lipid - independent
kinase activity and is uniquely positioned to phosphorylate target substrates throughout the cell ( not just on lipid membranes ) . -
pubmed
New thrombolytic agents , such as
reteplase ,
tenecteplase or desmoteplase , offer pharmacokinetic and dynamic advantages over recombinant
tissue plasminogen activator and have been or are currently being tested for safety and efficacy in clinical trials . -
pubmed
C1s prefers
Leu or
Val at P(3 ) and
Gly or
Ala residues at P(2 ) . -
pubmed
One ( 5C1 ) of the 13
mAb obtained was used as capture
antibody at 5 mug / ml and
biotin - labeled specific polyclonal antiserum at 0.63 microg / ml served for detection . -
pubmed
The key features of this approach are ( 1 ) the utilization of a Japp - Klingemann condensation / Fischer cyclization to prepare
cycloalkyl [ b ] indolones , ( 2 ) the asymmetric
borane reduction of these
heterocyclic ketones with
( S )- OAB to obtain enantiomerically pure
alcohols , and ( 3 ) the stereoselective
S ( N ) 2 - displacement of these
indole alcohol substrates with a
carbon nucleophile under Mitsunobu conditions to set the
C1 or
C3 tertiary
carbon stereocenter . -
pubmed
Two of the transporters ,
NaS1 and
NaS2 , carry substrates such as
sulfate ,
selenate and
thiosulfate . -
pubmed
The other members of the family (
NaDC1 ,
NaDC3 , and
NaCT ) are transporters for
di - and tri - carboxylates including
succinate ,
citrate and
alpha - ketoglutarate . -
pubmed
This review also discusses new members of the
SLC13 superfamily that exhibit both
sodium - dependent and
sodium - independent transport mechanisms . -
pubmed
Apical membrane
P2Y4 purinergic receptor controls
K + secretion by strial marginal cell epithelium . -
pubmed
BACKGROUND : It was previously shown that
K + secretion by strial marginal cell epithelium is under the control of
G - protein coupled receptors of the
P2Y family in the apical membrane . -
pubmed
Receptor activation by
uracil nucleotides (
P2Y2 ,
P2Y4 or
P2Y6 ) leads to a decrease in the electrogenic
K + secretion . -
pubmed
The present study was conducted to determine the subtype of the functional
purinergic receptor in gerbil stria vascularis , to test if receptor activation leads to elevation of intracellular [
Ca2 + ] and to test if the response to these receptors undergoes desensitization . -
pubmed
Activation caused a biphasic increase in intracellular [
Ca2 + ] that could be partially blocked by
2 - aminoethoxy - diphenyl borate (
2 - APB ) , an inhibitor of the
IP3 receptor and store - operated channels . -
pubmed
The ineffectiveness of
suramin at the concentration used was consistent with
P2Y4 but not
P2Y2 . -
pubmed
CONCLUSION : The results support the conclusion that regulation of
K + secretion across strial marginal cell epithelium occurs by
P2Y4 receptors at the apical membrane . -
pubmed
The apparent lack of desensitization of the response is consistent with two processes : a rapid - onset phosphorylation of
KCNE1 channel subunit and a slower - onset of regulation by depletion of plasma membrane
PIP2 . -
pubmed
CP27 - 215 was
15N labeled for NMR studies and a 2D 1H-15N - HSQC spectrum confirmed the presence of a well - ordered structure and the monomeric form of the protein . -
pubmed
We found that mutant
Sso1 proteins with insertions or duplications show limited function , whereas replacement of as few as three
amino acids preceding the transmembrane domain resulted in a nonfunctional SNARE in vivo . -
pubmed
Viability is also maintained when two
proline residues are inserted in the juxtamembrane of
Sso1p , suggesting that helical continuity between the transmembrane domain and the core coiled -
coil domain is not absolutely required . -
pubmed
The inability of
NR1 to assimilate
nitrate appears to be due to the lack of an active
nitrate reductase in these cells . -
pubmed
DI-6 culture growth was inhibited by 70 % at a
chlorate concentration of 30 micromolar , whereas growth of
NR1 was stimulated by more than 60 % on the same medium and by 100 % at a
chlorate concentration of 30 millimolar . -
pubmed
The results suggest that the deletions of the
N - glycans or other
amino acid substitutions in the
GP5 ectodomains of the mutants affect the immunogenicity of the neutralization epitope and the specificity of the
Abs raised to it but not the sensitivity of the virions to
Ab neutralization . -
pubmed
Aliskiren is the first in a new class of potent , orally effective
renin inhibitors . -
pubmed
Aliskiren produces dose - dependent BP reduction with few side effects and constitutes a novel pharmacologic approach to
renin -
angiotensin -
aldosterone inhibition . -
pubmed
Bicarbonate - induced stomatal closure as well as
H ( 2 ) O ( 2 ) production were restricted by exogenous
catalase or
diphenylene iodonium (
DPI , an inhibitor of
NAD ( P ) H oxidase ) . -
pubmed
In conclusion , the combination therapy with
Alendronate and
Alfacalcidol exhibited superiority in terms of
BMD , overall
fractures , rate of
falls and
back pain over either
Alendronate in combination with plain
vitamin D or
Alfacalcidol alone . -
pubmed
These results indicate that the
dichloromethane extract of PBL includes anticancer components composed of at least three
fatty acids , and apoptosis - inducing activity of the extract was mediated by
caspase-3 activation in
tumor cells . -
pubmed
We found : 1 ) the phagocytic activity of
hSP - A is reduced with increasing levels of in vivo
SP - A carbonylation ; 2 ) in vitro
ozone exposure of
hSP - A decreases its function in a dose - dependent manner as well as its ability to enhance phagocytosis of either gram - negative or gram - positive bacteria ; 3 ) the activity of both
SP - A1 and
SP - A2 decreases in response to in vitro
ozone exposure of proteins with
SP - A2 being affected more than
SP - A1 . -
pubmed
We conclude that both in vivo and in vitro oxidative modifications of
SP - A by carbonylation reduce its ability to enhance phagocytosis of bacteria and that the activity of
SP - A2 is affected more by in vitro
ozone - induced oxidation . -
pubmed
A correlation was seen ( R2=.911 ) with
FAS expression plotted against percentage change in
64Cu - ATSM uptake with C75 treatment . -
pubmed
CONCLUSIONS : Although
Cu - ATSM has clinical relevance in the PET imaging of
hypoxia in many
tumor types , its translation to the imaging of
prostate cancer may be limited by the overexpression of
FAS associated with
prostatic malignancies . -
pubmed
We found that
protein S significantly reduced acetylated
low - density lipoprotein (
AcLDL ) uptake and binding by macrophages and decreased the intracellular
cholesteryl ester content . -
pubmed
Soluble
Mer protein or
tyrosine kinase inhibitor
herbimycin A effectively blocked the effects of
protein S on AcLDL uptake . -
pubmed
Furthermore , the
hydroxy substitutions in the positions
C3 ( C - ring ) in
C5 and
C7 ( A - ring ) would be important for neuroprotection in this model . -
pubmed
RATIONALE : Little is known about the incidence of
isoniazid - associated
hepatitis in
HIV - infected Africans who receive both
isoniazid preventive therapy ( IPT ) and antiretroviral therapy (
ART ) . -
pubmed
INH - associated
hepatitis ( INH -
hepatitis ) was defined as having either
alanine or
aspartate aminotransferase greater than 5.0 times the upper limit of normal regardless of symptoms when
INH was not excluded as the cause . -
pubmed
Antiretroviral therapy (
ART ) was received by 480 participants but was not statistically associated with
INH -
hepatitis ( relative risk [ RR ] , 1.56 ; 95 % confidence intervals [ CI ] , 0.62 - 3.9 ) ; those receiving
nevirapine had a higher rate ( 2.0 % ) than those receiving
efavirenz ( 0.9 % ; P = 0.34 ) . -
pubmed
PTH - level reduction , which was significant versus baseline in both groups , although not statistically different between groups , was higher with
cinacalcet . -
pubmed
We investigated the effects of
angiotensin II (
ANG II)-dependent
hypertension on the platelet proteome . -
pubmed
Inhibition of
hepatitis C virus NS3 helicase by
manoalide . -
pubmed
Reaction pathway and free energy profile determined for specific recognition of
oligosaccharide moiety of
carboxypeptidase Y. -
pubmed
lectin , which interacts with the
carbohydrate moiety on a similar way . -
pubmed
No
post fixation was necessary for ICC , whereas , 20 minutes post fixation in
formalin ( 4 % ) was necessary for ISH . -
pubmed
Post fixation in
formalin is necessary for ISH . -
pubmed
BMPs signal via transmembrane type I and type
II serine /
threonine kinase receptors and intracellular
Smad effector proteins . -
pubmed
Serum
androgens , lipids ,
insulin resistance and
glucose metabolism measures were also determined . -
pubmed
,
thiophene ( T )- thienopyrazine (
TP ) , the electronic properties vary nonlinearly with D / A compositions . -
pubmed
[ Successful treatment of
trastuzumab - resistant
HER2 - positive
breast cancer with extensive
liver metastases using the combination of
trastuzumab and
capecitabine - a case report ] . -
pubmed
RESULTS : In
PD population , there were no statistical relationships between early and delayed ( 123)I MIBG cardiac and
( 123 ) I FP -
CIT striatal uptake in contralateral caudate ( P > 0.05 ) and in contralateral putamen ( P > 0.05 ) to the side mainly affected ; no statistically significant relationships have been found at any level when considering ipsilateral striatum . -
pubmed
All three mast cell populations were rendered hyporesponsive by ES-62 and whilst the mechanisms underlying such desensitisation have not been fully delineated , they reflect a downregulation of
calcium and
PKC alpha signalling . -
pubmed
coli O157 : H7 , over - expression of
PDE decreased
c - di - GMP level . -
pubmed
We further observed decreased curli fimbriae synthesis in the
strain with decreased
c - di - GMP levels , which was supported by the reduction in the transcription of curli large subunit gene
csgA and the curli expression regulator gene
csgD. -
pubmed
In fact , two
histone deacetylase inhibitors ( HDIs ) ,
SAHA and
Depsipeptide , are FDA approved for single - agent treatment of refractory
cutaneous T cell lymphoma (
CTCL ) . -
pubmed
Evaluation of
C - reactive protein before and on - treatment as a predictor of benefit of
atorvastatin : a cohort analysis from the Anglo - Scandinavian Cardiac Outcomes Trial lipid - lowering arm . -
pubmed
OBJECTIVES : The aim of this study was to determine whether baseline and on - statin
C - reactive protein (
CRP ) are independent predictors of cardiovascular ( CV ) outcome beyond
low - density lipoprotein cholesterol (
LDL - C ) . -
pubmed
After 6 months of
atorvastatin therapy , the median
LDL - C and
CRP were reduced by 38.7 % and 25.8 % , respectively . -
pubmed
In the circulation
25 - hydroxyvitamin D (
25 ( OH ) D ) is bound to
vitamin D - binding protein (
DBP ) and
albumin . -
pubmed
Serum
25 ( OH ) D ,
DBP and
albumin were measured and the free and bio - available ( free +
albumin - bound )
25 ( OH ) D calculated . -
pubmed
Based on genotyping of the polymorphisms rs7041 and rs4588 , the six common
DBP phenotypes were identified and the free and bio - available
25 ( OH ) D calculated according to the corresponding binding coefficients . -
pubmed
Relations between measures of
25 ( OH ) D and
PTH and
BMD were evaluated with linear regression adjusted for age and
BMI . -
pubmed
Adjusting for
DBP phenotype affected the calculated free and bio - available
25 ( OH ) D levels up to 37.5 % . -
pubmed
All measures of
25 ( OH ) D correlated significantly with
PTH , whereas a significant association with
BMD was only seen for the free and bio - available
25 ( OH ) D measures . -
pubmed
A role beyond learning for
NMDA receptors in reward - based decision - making - a pharmacological study using
d - cycloserine . -
pubmed
Immune cells , stimulated by
NEFAs , produce pro - inflammatory
cytokines , which have a direct effect on oxidating radicals that directly target the release of
noradrenalin . -
pubmed
Cement distribution ( 2 ml
PMMA ) was analyzed on
CT . -
pubmed
Mutations in the
KCNJ13 gene that encodes the inwardly rectifying
potassium channel
Kir7.1 cause
snowflake vitreoretinal degeneration (
SVD ) and
leber congenital amaurosis (
LCA ) . -
pubmed
Kir7.1 controls the microenvironment between the photoreceptors and the retinal
pigment epithelium (
RPE ) and also contributes to the function of other organs such as uterus and brain . -
pubmed
We have determined that the mutation results in a severe truncation of the
Kir7.1 C - terminus , alters protein localization , and disrupts
potassium currents . -
pubmed
RPTPsigma is reciprocally regulated by interactions with chondroitin
sulfate or
heparan sulfate containing extracellular proteoglycans in a mechanism called the proteoglycan switch . -
pubmed
Osteoconductivity on these scaffolds was investigated by the common osteogenic markers
alkaline phosphatase (
ALP ) activity ,
calcium mineral deposition and bone - related gene activation . -
pubmed
CONCLUSIONS : Osteoconductivity of
PES nanofibres was markedly enhanced after coating them with
BG , and introduction of this construct as new bone - graft substitute for
bone loss and defects is indicated . -
pubmed
Incubation of bone chips with 0.2 %
chlorhexidine , followed by vigorously washing resulted in a BCM with even higher expression of
IL11 ,
PRG4 and
NOX4 . -
pubmed
Chlorhexidine alone , at low concentrations , increased
IL11 ,
PRG4 and
NOX4 expression , independent of the
TGF - beta receptor I kinase activity . -
pubmed
Quantitative
hepatitis B surface antigen combined with
hepatitis B e antigen as sustained virological response predictors during extended therapy with
Peginterferon alfa-2a for
hepatitis B e antigen - positive
chronic hepatitis B. -
pubmed
The serum
HBsAg cut - off of 1800IU / mL at week 48 had area under curve ( AUC ) of 0.727 , and the
serum HBsAg cut - off of 1500IU / mL , combined with
HBeAg loss at week 72 , had AUC of 0.753 to predict SVR during the follow - up . -
pubmed
The results in our study provide the first experimental evidence that both
caspase - dependent and
AIF - dependent apoptosis pathways are involved in the loss of the nigral DA neurons caused by intrastriatal administration of
6 - OHDA , and intrastriatal preadministration of
Bip - V5 can inhibit the above two apoptosis pathways to protect the nigral DA neurons . -
pubmed
The hormone
islet amyloid polypeptide (
IAPP , or
amylin ) plays a role in
glucose homeostasis but aggregates to form islet amyloid in
type-2 diabetes . -
pubmed
Activation of the inflammasome , defects in autophagy ,
ER stress , generation of reactive
oxygen species , membrane disruption , and receptor mediated mechanisms have all been proposed to play a role . -
pubmed
We engineered an
aTF , the
Escherichia coli lac repressor ,
LacI , to respond to one of four new inducer molecules :
fucose ,
gentiobiose ,
lactitol and
sucralose . -
pubmed
Using computational protein design , single - residue saturation mutagenesis or random mutagenesis , along with multiplex assembly , we identified new variants comparable in specificity and induction to wild - type
LacI with its inducer ,
isopropyl beta - D-1 - thiogalactopyranoside (
IPTG ) . -
pubmed
This article highlights the different types of images that can be generated by using dual - energy
CT protocols such as virtual monochromatic , virtual unenhanced ( ie , water ) , and pulmonary blood volume ( ie ,
iodine ) images . -
pubmed
Elevated
UCP2 levels also protected against excitotoxic cell
death induced by intraocular injection of either
NMDA or
kainic acid . -
pubmed
In vitro release study of
PL - DOC showed that
PL - DOC had a sustained release effect . -
pubmed
Non -
nucleoside reverse transcriptase inhibitor ( NNRTI ) resistance was detected in 17 of 26 ( 65 % ) patients , 2 ( 7 % ) had
Thymidine analogue mutations , and 3 ( 11 % ) had K65R. -
pubmed
Recent work has established that , in the brain and other systems , the
GR is translocated from the cytosol to the mitochondria and that stress and
corticosteroids have a direct influence on mtDNA transcription and mitochondrial physiology . -
pubmed
ChIP sequencing of
GR showed that
corticosterone treatment induced a dose - dependent association of the
GR with the control region of the mitochondrial genome . -
pubmed
Moreover , 19 protein bands from
SDS - PAGE were analyzed and a total of 45 different proteins were identified by LC -
MS /
MS . -
pubmed
PARP inhibitor
4 - aminobenzamide (
4 - AB ) was administered daily for ten weeks after
diabetes induction . -
pubmed
PARP-1 activity ,
reduced glutathione (
GSH ) and
nitrite contents were assessed in heart muscle . -
pubmed
Fasting
glucose ,
fructosamine ,
insulin , and
tumor necrosis factor alpha (
TNF - alpha ) levels were measured in serum . -
pubmed
The pore size distributions calculated using the
nitrogen adsorption isotherms and
TEM images show that all
carbons are mainly nanoporous with certain contribution of narrow mesopores ( at pore half - width x & lt ; 5 nm ) . -
pubmed
Mo polyoxometalate nanoparticles inhibit
tumor growth and
vascular endothelial growth factor induced angiogenesis . -
pubmed
Furthermore , western blot analysis of cell signaling molecules indicated that
Mo POMs blocked the
vascular endothelial growth factor receptor 2 - mediated
ERK1 / 2 and
AKT signaling pathways in endothelial cells . -
pubmed
One day
post -
SAH , brain tissue damage was measured with
T2 - weighted magnetic resonance imaging , followed by cortical application of 1 mol / L
KCl ( to induce SDs ) or
NaCl ( no SDs ) . -
pubmed
In mESCs ,
acetyl -
LIFR undergoes homodimerization , leading to
STAT3 hypo- or hyper - activation depending on the presence or absence of
gp130 . -
pubmed
Thus ,
LIFR acetylation and
serine phosphorylation differentially promote stem cell self - renewal and differentiation . -
pubmed
Synucleinopathies are
neurodegenerative diseases characterized by the abnormal accumulation of
alpha - synuclein (
alpha - Syn , a 140
amino acid protein that normally plays various neurophysiologic roles ) aggregates . -
pubmed
However , under
ABA , it showed higher levels of both
peroxidases . -
pubmed
Morphology of skin tissue was observed with
HE staining ;
tumor necrosis factor - alpha (
TNF - alpha ) content in serum and skin tissue was determined by enzyme - linked immunosorbent assay ;
superoxide dismutase (
SOD ) content in serum and skin tissue was determined by
hydroxylamine method ;
malondialdehyde content in serum and skin tissue was determined by
thiobarbituric acid method . -
pubmed
CONCLUSIONS : The ultimate failure load was significantly greater in the
F - MMA than the TSS group and similar to the conventional
MMA technique . -
pubmed
Furthermore , the proposed method was successfully applied to detect
Co ( II ) in living cell samples , and the results were agreed with those obtained by the standard ICP -
MS method . -
pubmed
We also show that
SAA could increase the chemotherapeutic efficacy of
cisplatin , suggesting a synergistic effect of
SAA and
cisplatin . -
pubmed
Thyroglobulin antibodies (
TG - aAb ) ,
thyroid peroxidase antibodies (
TPO - aAb ) ,
TSH , free T3 , free T4 , total T3 , and total T4 , as well as urine
iodine excretion were determined . -
pubmed
Se status was assessed by serum
Se and
selenoprotein P (
SELENOP ) concentrations . -
pubmed
RESULTS : Serum
Se and
SELENOP concentrations were relatively low in both control and
AITD women . -
pubmed
The decline in
TPO- and
TG - aAb was unrelated to the
Se status . -
pubmed
Conditions that favor the helical structure formation in structurally similar
sophorolipids ( SLs ) , that is ,
elaidic acid SLs ( having a trans double bond between the
C9 and
C10 positions of the
alkyl chain ) and
stearic acid SLs ( no double bond ) , are presented here . -
pubmed
Despite new studies comparing
insulin pump therapy to multiple daily injections , its effectiveness in improving
glucose and pregnancy outcomes remains unclear . -
pubmed
The long - term course of
PRP symptoms was also examined , along with current (
today ) levels of trait anxieties . -
pubmed
By matching the redox capacity of the
PTMA- co - BP thin film and
bis ( alkoxy )- substituted poly ( propylenedioxythiophene ) , the
ECD achieved an optical contrast of 72 % in a small potential window of 2.55 V ( i.e. -
pubmed
Novel approach to quorum quenching : rational design of antibacterials in combination with
hexahistidine - tagged
organophosphorus hydrolase . -
pubmed
In this article the
lactonase activity of the preparations based on
hexahistidine - tagged
organophosphorus hydrolase (
His6 - OPH ) towards AHLs was studied . -
pubmed
Initially , three of the most interesting
beta - lactam antibiotics were selected from seven that were trialed during molecular docking to
His6 - OPH . -
pubmed
The
lactonase activity of the preparations was investigated under different physical - chemical conditions in the hydrolysis of AHLs [
N - butyryl - D , L - homoserine lactone ,
N -( 3 - oxooctanoyl )- D ,
L - homoserine lactone ,
N -( 3 - oxododecanoyl )- L - homoserine lactone ] . -
pubmed
The broadening of the catalytic action of the
enzyme against AHLs was revealed in the presence of the
meropenem . -
pubmed
Results of molecular docking of AHLs to the surface of the
His6 - OPH dimer in the presence of antibiotics allowed proposing the mechanism of such interference based on a steric repulsion of the
carbon chain of hydrolyzed AHLs by the antibiotics bounded to the enzyme surface . -
pubmed
Cisplatin (
CP ) is one of the most potent anti -
cancer drugs used against different types of
cancer . -
pubmed
Urine and blood samples were collected for the evaluation of kidney function including serum
creatinine ,
BUN ,
cystatin - c , and
creatinine clearance . -
pubmed
We noticed that both doses of
tempol significantly improved kidney function , which was deteriorated by
CP injection . -
pubmed
Tempol significantly elevated kidney
glutathione (
GSH ) content and
SOD activity , and decreased kidney lipid peroxidation and NOx production . -
pubmed
Tempol also significantly decreased kidney
caspase-3 expression which was elevated by
CP toxicity . -
pubmed
Thus , we conclude that
tempol can protect against
CP nephrotoxicity . -
pubmed
We noticed that both doses of
tempol are effective in ameliorating
CP -
nephrotoxicity . -
pubmed
Treatment with LO for 8 weeks induces a mean reduction of
13 C - ALA conversion to
13 C - DHA by 48 % as reflected by the
LDL compartment , and a mean reduction of the
13 C - ALA incorporation into
LDL by 46 % . -
pubmed
CONCLUSION : A 2 - month dietary intake of a high dose of LO is sufficient to reach saturation of
ALA incorporation into
LDL particles , which are responsible for
ALA distribution in the body . -
pubmed
Inhibition of
nitric oxide (
NO ) production significantly increased the sensitivity of cells from the normal pregnancies to
TNFalpha but not in the high risk group establishing a functional role for
NO in this system . -
pubmed
[
Indoleamine 2,3 - Dioxygenase Activity during Long - Term
Letrozole Therapy for
Hormone Receptor - Positive
Breast Cancer ] . -
pubmed
We evaluated the significance of
indoleamine 2,3 - dioxygenase(
IDO)during
letrozole therapy in long - term response . -
pubmed
We measured
IDO activity based on the
tryptophan(
Trp)/
kynurenine(
Kyn)ratio . -
pubmed
Recently identified
imidazoline receptors of the first type (
I1Rs ) on the cardiomyocyte 's sarcolemma open a new field in
calcium signaling research . -
pubmed
Here we investigated the effects of
I1Rs activation on L - type voltage - gated
Ca2 + -currents under catecholaminergic stress induced by the application of beta - agonist ,
isoproterenol . -
pubmed
Pharmacological agonist of
I1Rs (
I1 - agonist ) ,
rilmenidine , and the putative endogenous
I1 - ligand ,
agmatine , have been shown to effectively reduce
Ca2 + -currents potentiated by
isoproterenol . -
pubmed
Inhibitory analysis shows that the ability to suppress voltage - gated
Ca2 + -currents by
rilmenidine and
agmatine is fully preserved in the presence of the
protein kinase A blocker (
PKA ) , which indicates a
PKA - independent mechanism of their action . -
pubmed
The blockade of
NO synthase isoforms with
7NI does not affect the intrinsic effects of
agmatine and
rilmenidine , which suggests
NO - independent signaling pathways triggered by
I1Rs . -
pubmed
A nonspecific
serine / threonine protein phosphatase (
STPP ) inhibitor ,
calyculin A , abrogates effects of
rilmenidine or
agmatine on the
isoproterenol - induced
Ca2 + -currents . -
pubmed
TAT peptide - modified
cisplatin - loaded
iron oxide nanoparticles for reversing
cisplatin - resistant
nasopharyngeal carcinoma . -
pubmed
On this basis , modification with
TAT peptide ( YGRKKRRQRRR ) significantly improved
tumor intracellular uptake , devoting to better curative effects and minimized side effects by reducing
CDDP therapeutic doses . -
pubmed
The sandwich fragments are
eta2 - coordinated to the second metal center with the average M - C distances of 2.837(4 ) A and 2.879(5 ) A for
yttrium and
lanthanum complexes , respectively . -
pubmed
Investigations on the Relationship between Ovarian , Endocrine , and Renal Findings in Nonclinical Safety Studies of the
gamma - secretase Inhibitor
Avagacestat . -
pubmed
Of note , these pro- or anti - EndMT effects were reversed by the
EP4 overexpression or the
EP4 antagonist
AH-23848 , respectively . -
pubmed
Polymorphisms in
CEP72 have been linked to increased incidence of
vincristine - induced
toxicities , namely
peripheral neuropathy . -
pubmed
Hepatic expression of genes involved in
inflammation , as well as
glucose and lipid metabolism , were determined by quantitative
polymerase chain reaction . -
pubmed
During the PAP , EKD showed a more pronounced increase in
glucose levels at the first hour after LPS challenge compared with the
CD - LPS group . -
pubmed
The Influence of Glycans - Specific Bioconjugation on the
FcgammaRI Binding and In vivo Performance of
89Zr - DFO - Pertuzumab . -
pubmed
To circumvent this issue , we have previously developed a chemoenzymatic bioconjugation strategy that site - specifically appends cargoes to the biantennary heavy chain glycans attached to
CH2 domains of the
immunoglobulin 's Fc region . -
pubmed
Methods : We synthesized three
desferrioxamine (
DFO)-labeled immunoconjugates based on the
HER2 - targeting antibody
pertuzumab : one using random bioconjugation methods (
DFO - nsspertuzumab ) and two using variants of our chemoenzymatic protocol ( DFO - sspertuzumab - EndoS and
DFO - sspertuzumab - betaGal ) . -
pubmed
Results : MALDI - ToF and
SDS - PAGE analysis confirmed the site - specific nature of the bioconjugation , and flow cytometry and surface plasmon resonance (
SPR ) revealed that all three immunoconjugates bind
HER2 as effectively as native
pertuzumab . -
pubmed
Critically , however ,
SPR experiments also illuminated that
DFO - sspertuzumab - EndoS possesses an attenuated binding affinity for
huFcgammaRI ( 17.4 + - 0.3 nM ) compared to native
pertuzumab ( 4.7 + - 0.2 nM ) ,
DFO - nsspertuzumab ( 4.1 + - 0.1 nM ) , and
DFO - sspertuzumab - betaGal ( 4.7 + - 0.2 nM ) . -
pubmed
Neutralization of lysosomal pH effectively reduced
ZVI@
Ag NP - induced programmed cell
death . -
pubmed
Our results revealed the successful preparation of AE - loaded
folate - targeted nanoparticles (
NP - F - AE ) with particle size around 177 nm , negative zeta potential , polydispersity index < 0.20 , and higher efficiency of encapsulation for both GAs present in the extract ( > 85 % ) . -
pubmed
To investigate the cellular uptake , the fluorescent dye
coumarin-6 was encapsulated into the nanoparticle (
NP - F -
C6 ) . -
pubmed
The study found that
EX-527 reversed OVA - induced
airway inflammation , and reduced OVA - induced increases in inflammatory
cytokine expression , and total cell and eosinophil counts in bronchoalveolar lavage fluid . -
pubmed
All effects induced by
EX-527 , including increased phosphorylated -
mTOR and decreased autophagy , were abrogated by
rapamycin treatment . -
pubmed
Apoptotic and antihepatofibrotic effect of
honokiol via activation of
GSK3beta and suppression of
Wnt /
beta - catenin pathway in hepatic stellate cells . -
pubmed
Honokiol increased sub
G1 population and activated
caspase 3 and
cleaved poly ( ADP - ribose ) polymerase (
PARP ) in HSCs . -
pubmed
Moreover ,
honokiol attenuated the expression of
alpha smooth muscle actin (
alpha - SMA ) ,
transforming growth factor beta 1 (
TGF - beta1 ) ,
phospho -
Smad3 ,
phospho -
AKT ,
cyclin D1 ,
c - Myc ,
Wnt3a ,
beta - catenin , and activated phosphorylation of
glycogen synthase kinase 3 beta (
GSK3beta ) in HSCs . -
pubmed
Overall ,
honokiol exerts apoptotic and antifibrotic effects via activation of
GSK3beta and inhibition of
Wnt3a /
beta - catenin signalling pathway . -
pubmed
NSUN2 is an RNA methyltransferase responsible for the
m5C modification of multiple RNAs . -
pubmed
Mechanistically , we determined that
Pkm2 interacts with
beta - catenin (
Ctnnb1 ) in the cytoplasm of CM , inhibiting
Ctnnb1 phosphorylation at
serine 552 and
tyrosine 333 , by
Akt . -
pubmed
The accumulation of unrepaired
ribonucleotides is associated with
genomic instability , which is mediated by
DNA topoisomerase 1 (
Top1 ) processing of embedded
ribonucleotides . -
pubmed
The cleavage initiated by
Top1 at the site of a
ribonucleotide leads to the formation of a
Top1 - DNA cleavage complex (
Top1cc ) , occasionally resulting in a DNA double - strand break ( DSB ) . -
pubmed
BACKGROUND AND AIMS : Cholangiocytes express
cystic fibrosis transmembrane conductance regulator (
CFTR ) which is involved in
bicarbonate secretion for the protection against
bile toxicity . -
pubmed
Thus , the aim of this study is to investigate the effect of
hypoxia on
CFTR activity in intrahepatic cholangiocyte organoids ( ICOs ) and test drug - interventions to restore
bicarbonate secretion . -
pubmed
Under oxygenated conditions , both
CFTR and
ANO1 channels were responsible for
forskolin and
UTP activated anion - secretion . -
pubmed
Forskolin - stimulation in absence of intracellular
chloride showed ion - transport , indicating that
bicarbonate could be secreted by
CFTR . -
pubmed
Switching from
oxygen to
hypoxia during
CFTR - measurements , reduced
CFTR activity ( p=0.03 ) . -
pubmed
Cholesterol depletion , which was achieved by a mild physiological treatment , and which increased the membrane - lipid fluidity , resulted in a substantial increase in the average number of the available
transferrin receptors per bone marrow cell . -
pubmed
Response of plasma
insulin to small doses of
tolbutamide in
obesity and
acromegaly . -
pubmed
In
acromegaly the
insulin release pattern is compatible with an increased number of secretory units which , however , are relatively insensitive to
tolbutamide , probably due to an elevated
serum growth hormone level . -
pubmed
In addition , secondary B cells can be stimulated in collaboration with allogeneic
antigen - specific T cells to yield clones producing antibody of the
IgG1 isotope , whereas primary B cells similarly stimulated produce only
IgM antibodies . -
pubmed
She had undergone
R2 curative subtotal gastrectomy in June 1985 , and had orally taken
tegafur 600 mg / day and then changed to
doxifluridine 800 mg / day as postoperative adjuvant chemotherapy . -
pubmed
Five months after the operation she had back
pains and both
99mTc - MDP and
67Ga - citrate scintigram showed
L1 vertebra and rib bone
metastasis . -
pubmed
However , at 0.1 - 1.0 microM ,
verapamil prevented or attenuated prolongation of transmural
CT by ischemic conditions and reperfusion . -
pubmed
Transmural
CT was further prolonged at 3 microM
verapamil . -
pubmed
The inhibitory activity of
CD4 + Leu-8 + T cell supernatants could not be accounted for by a deficiency or excess of
IL-2 ,
IL-4 ,
IFN - gamma ,
IL-6 , or
PGE2 . -
pubmed
A Radial - Pak microBondapak
C18 reversed - phase cartridge was used for the separations and elutions were carried out with
acetonitrile - water -
methanol -
acetic acid mixtures . -
pubmed
rpoH111 was a point mutation resulting in a single
amino acid substitution . -
pubmed
In common with other heavy
SR preparations , it contains predominantly the
calcium pump membrane ( 80 - 85 % of the membrane ) and compartmental contents ( CC ) , consisting mainly of
calcium - binding protein (
calsequestrin ) . -
pubmed
Effects of
disodium ethane-1 - hydroxy-1,1 - diphosphonate (
EHDP ) on
interleukin 1 production by macrophages . -
pubmed
A high dose ( 1 mg / ml ) of
EHDP inhibited
interleukin 1 (
IL 1 ) production by oil - induced peritoneal macrophages stimulated with
muramyl dipeptide (
MDP ) , lipopolysaccharide ( LPS ) ,
phorbol myristic acetate (
PMA ) , heat - aggregated
IgG2 or
calcium ionophore
A23187 . -
pubmed
On the other hand , low doses ( less than 0.125 mg / ml ) of
EHDP augmented the
MDP induced
IL 1 production by macrophages . -
pubmed
This biphasic effect was also observed when macrophages were exposed to
EHDP at 37 C for 24 hr and then stimulated with
IL 1 inducers . -
pubmed
These results demonstrated that
EHDP acted on macrophages directly and modulated
IL 1 production in vitro . -
pubmed
[
Hormone receptor determination in
breast cancer using the dextran- coated
charcoal method and monoclonal antibodies : correlation with cytomorphologic grading ] . -
pubmed
In this study , cells derived from primitive
neuroectodermal tumors of the posterior fossa (
PNET - PF ) were examined for their sensitivity to
LAK cytolysis utilizing an in vitro 4 - hour
chromium-51 - release assay . -
pubmed
Flow cytometric measurements of
Ca antigen (
Ca ) visualized by immunofluorescence with Ca1 monoclonal antibody ( Ca1 ) were performed on cells from 26
urinary bladder carcinomas of various histologic grades and DNA ploidy and on 6 samples of morphologically
normal urothelium . -
pubmed
Chromatography is performed on a reversed - phase
C18 column , connected with a fixed - wavelength ultraviolet detector at 280 nm ; the compounds are eluted in about 5 min with
tetrahydrofuran - acetonitrile-0.01 M
sodium phosphate buffer , pH 7.4 ( 12:25:63 , v / v / v ) . -
pubmed
Inactivation of
melanocyte - stimulating hormone release - inhibiting factor by a
manganese - stimulated
bovine brain aminopeptidase . -
pubmed
Although mitotic rate was subnormal , several other factors attest to the essential viability of the cultured intestine :
L - leucine incorporation into protein , as well as the synthesis of a specific
vitamin D ( 3 )-induced
calcium - binding protein (
CaBP ) , increased over a 48 - h culture period , and the electropotential gradient across the intestine was maintained throughout the culture period as was a concentration gradient for
calcium . -
pubmed
The tissue responded to
vitamin D ( 3 ) in the medium by synthesizing the
calcium - binding protein within 6 h and by exhibiting enhanced ( 45)
Ca uptake within 12 - 24 h. Concentrations of
vitamin D ( 3 ) , or its 25 - hydroxylated derivative , higher than necessary for
CaBP induction , also increased the activity of
alkaline phosphatase . -
pubmed
The
1,25 - dihydroxylated derivative of vitamin D ( 3 ) , at a level extremely potent in
CaBP induction , did not stimulate
alkaline phosphatase . -
pubmed
Other embryonic organs , esophagus , stomach , liver , pancreas , lung , skin , and muscle , did not produce
CaBP in response to
vitamin D ( 3 ) in the culture medium . -
pubmed
Fine - structural localization of
acid phosphatase has been studied in
triparanol - treated adrenal glands . -
pubmed
Lead phosphate reaction product ( indicating
acid phosphatase activity ) is demonstrable within these membrane - limited cytoplasmic bodies , identifying them as morphological lysosomes . -
pubmed
Urinary
kallikrein activity , renal hemodynamics , and
electrolyte handling during chronic beta blockade with
propranolol in
hypertension . -
pubmed
In the in vitro tests , both groups of agonists inhibited electrically evoked activity ; however in these experiments only antagonism of opiates by
naloxone and alpha 2 - agonists by
yohimbine could be shown and it is concluded that in these systems the activity of the alpha 2 - agonists is mediated via the presynaptic
alpha 2 - receptors only . -
pubmed
Experiments have been carried out to provide direct evidence for the proposed presynaptic
5 - HT autoreceptor agonist activity of
8 - hydroxy-2 -( di - n - propylamino ) tetralin (
8 - OH - DPAT ) a compound with selectivity for the
5 - HT1A subtype of the
5 - HT1 binding site . -
pubmed
These experiments have therefore failed to provide direct evidence for agonist activity of
8 - OH - DPAT at the
5 - HT autoreceptor and alternative explanations must be sought for its biochemical and behavioural effects in vivo . -
pubmed
Moreover , the fact that
8 - OH - DPAT is inactive at the
autoreceptor at concentrations selective for the
5 - HT1A recognition site suggests that this subtype of the
5 - HT1 binding site may not correspond to the
5 - HT autoreceptor . -
pubmed
Plasma renin substrate concentration during chronic
propranolol therapy . -
pubmed
GTP - insensitive
ornithine decarboxylase in acetobacteria able to synthesize
spermine . -
pubmed
However , their
S - adenosyl - L - methionine decarboxylase resembled other prokaryotic
adenosylmethionine decarboxylases in requiring
Mg2 + ions in vitro for full activity . -
pubmed
Plasma
cholesterol levels rose sharply in the HCO and SFO groups but not at all in the
EPO group . -
pubmed
EPO contains the product of delta-6 - desaturation ,
18:3n-6 , suggesting that conversion of
linoleic acid to
18:3n-6 and possibly to further metabolites may be important for the
cholesterol - lowering effect of
polyunsaturates . -
pubmed
These findings together with our results which show inhibition of
AR by
tyrosine - phosphorylation inhibitors , indicate that ejaculated bull sperm contain a typical 170 - kDa
EGFR which is active in the mechanism leading to
AR . -
pubmed
This selection consisted of the absence in vitro of genomes with certain
amino - acid substitutions at or near conserved glycosylation sites of the
C2 region at positions 276 and 289 . -
pubmed
Quantitative autoradiography was used to assess alterations in
serotonin ( 5 - HT ) receptor binding in the hypothalamus and hippocampus following denervation with
5,7 - dihydroxytryptamine (
5,7 - DHT ) , ovariectomy ( OVX ) and gonadal
steroid manipulation . -
pubmed
In addition , there was a significant interaction between OVX and
5,7 - DHT for both the
5 - HT1a receptor and the
5 - HT transporter in the VMN , such that OVX inhibited the
5,7 - DHT - induced increase in
5 - HT1a receptors and attenuated the
5,7 - DHT - induced decrease in
5 - HT transporter binding . -
pubmed
In a separate experiment the effect of gonadal
steroid manipulation on
5 - HT receptor binding was assessed . -
pubmed
Subtype - specific binding of
azidoanilido - GTP by purified
G protein alpha subunits . -
pubmed
Azidoanilido - GTP ( AA -
GTP ) , a hydrolysis - resistant , photoreactive
GTP analog , is becoming an increasingly popular tool for identifying activation of specific
G proteins by receptors within native plasma membranes . -
pubmed
Despite the use of
AA - GTP as an affinity probe , surprisingly little is known regarding the ability of various
G protein alpha subunits to bind this analog . -
pubmed
To directly address this issue , we compared the ability of four purified
G protein alpha subunits (
Go , Gi2 , Gs , and Gz ) to bind AA -
GTP with their ability to bind
GTP gamma S , a
GTP analog commonly used to characterize the
GTP - binding properties of
G proteins . -
pubmed
All four
G alpha subunits tested bound
AA - GTP in a manner distinct from their binding of
GTP gamma S. -
pubmed
One of these proteins ,
Gs alpha , required millimolar levels of free
Mg2 + for significant binding of AA -
GTP , while
Go alpha and
Gi alpha 2 displayed peak AA -
GTP binding at approximately 100 microM free
Mg2 + . -
pubmed
The fourth
G alpha subunit ,
Gz , bound AA -
GTP very poorly relative to
GTP gamma S regardless of the
magnesium concentration . -
pubmed
These results indicate that individual
G protein alpha subunits differ markedly in their ability to bind AA -
GTP . -
pubmed
Use of AA -
GTP to identify specific
G protein - receptor interactions must therefore take into account the varied abilities of
G alpha subunits to bind this analog . -
pubmed
Bovine serum albumin (
BSA ) is used as acceptor of free
fatty acids in the incubation medium of isolated fat cells . -
pubmed
The assay involves pretreatment with the detergent
sodium dodecyl sulfate (
SDS ) to liberate the free
fatty acids from the
bovine serum albumin before activation by
acyl - CoA synthetase (
ACS ) (
EC 6.2.1.3 ) . -
pubmed
This is followed by oxidation of the resulting
thioesters by
acyl - CoA oxidase (
ACO ) . -
pubmed
The
H2O2 formed is subsequently measured in a
horseradish peroxidase (
HRP ) (
EC 1.11.1.7)-catalyzed
luminol reaction . -
pubmed
No interference was observed up to the following concentrations : 5 microM
epinephrine , 5 microM
norepinephrine , 80 microM
isoproterenol , 1 mM
insulin , 2.5 mM
propranolol , 5 mM
phentolamine , and 5 microM
ascorbate . -
pubmed
Associations with serum concentrations of total ,
low - density lipoprotein (
LDL ) ,
high - density lipoprotein-2 (
HDL2 ) , and
HDL3 cholesterol ,
apolipoproteins ( apos ) A - I and B ,
lipoprotein ( a ) [
Lp ( a ) ] were studied . -
pubmed
Serum total and free
testosterone concentrations were positively correlated with
HDL and
HDL2 cholesterol and
apo A - I. -
pubmed
Serum total and free
estradiol levels showed borderline - significant negative associations with total and
LDL cholesterol levels . -
pubmed
Among urinary variables , total
estradiol excretion was negatively associated with
apo B levels and showed borderline - significant associations with total and
LDL cholesterol . -
pubmed
Adjustment for potential confounders , including age ,
body mass index (
BMI ) , and waist to hip ratio ( WHR ) , strengthened the associations between urinary total
estradiol and serum total
cholesterol ,
LDL cholesterol , and
apo B. -
pubmed
Urinary free
estradiol showed a significant correlation with
HDL3 cholesterol . -
pubmed
Urinary excretion of total
testosterone was significantly negatively associated with serum
cholesterol and
LDL cholesterol levels only after controlling for confounding variables . -
pubmed
HoloPYP could be reconstituted by the addition of
p - coumaric anhydride to the
histidine - tagged
apoPYP (
PYP lacking its chromophore ) . -
pubmed
No association found between
Alzheimer ' s disease and a mitochondrial
tRNA glutamine gene variant . -
pubmed
In contrast , purified
NS3 in which the predicted catalytic
serine has been mutated loses
protease but retains
ATPase activity . -
pubmed
Our results support the concept that
HLAL and
HGL /
RLL are members of a gene family of
lipases that most likely have evolved by duplication of an ancestral gene and subsequently assumed distinct roles in neutral
lipid metabolism due to sequence divergence and different expression patterns . -
pubmed
When control PMNs were tested , active UC rectal dialysate was almost as effective ( +115 % ) as
N - formyl - methionyl - leucyl - phenylalanine ( +132 % ) in up - regulating
CD11b . -
pubmed
Changes in intracellular
calcium levels paralleled these changes in
CD11b . -
pubmed
Rectal dialysate - induced changes in both
CD11b and
calcium paralleled our previous findings on rectal dialysate - induced changes in ROS production . -
pubmed
Exposure of macrophages to
TGF - beta1 led to a rapid and sustained increase in
uPA mRNA levels , which was independent of de novo protein synthesis and completely inhibited by
actinomycin D. -
pubmed
Likewise ,
okadaic acid potentiated the ability of
TGF - beta to up - regulate macrophage
uPA expression . -
pubmed
One copy of MeIARV was found to be inserted at the end of the 6th
leucine domain of the c - maf proto - oncogene , which encodes a
basic region / leucine zipper transcription factor related to the
AP-1 family that is able to form
homodimers or heterodimers with
Fos and
Jun transcription factors . -
pubmed
Nitric oxide - induced downregulation of
Cdk2 activity and
cyclin A gene transcription in vascular smooth muscle cells . -
pubmed
The addition of
SNP or
SNAP to VSMC cultures at the time of serum stimulation abrogates the induction of
cdk2 activity without suppressing protein levels of
cdk2 or
cyclin E. -
pubmed
These
NO donors block serum - stimulated upregulation of
cyclin A mRNA and protein and repress the serum induction of
cyclin A promoter activity in VSMCs . -
pubmed
CONCLUSIONS : The addition of the
nitric oxide donors
SNP or
SNAP to mitogen - stimulated VSMCs prevents activation of
cdk2 , a key regulator of the
G1 and S phases of the cell cycle . -
pubmed
These
NO donors do not affect the expression of
cdk2 protein but block the mitogen - induced expression of
cyclin A , an activating subunit of
cdk2 . -
pubmed
SNP and
SNAP also repress the mitogen - stimulated activation of the
cyclin A promoter . -
pubmed
These data suggest that the antiproliferative effect of
NO on VSMCs results , at least in part , from the repression of
cyclin A gene transcription . -
pubmed
Overall , the findings from the present study provide additional evidence that there is basolateral uptake of inorganic
mercury in the kidneys and that the primary mechanism involved in this basolateral uptake is dependent on the activity of the
organic anion transporter . -
pubmed
The
dose - limiting toxicity (
DLT ) of
UFT has generally been
diarrhea . -
pubmed
Enzyme solubilization by
digitonin , affinity chromatography purification and ultrafiltration underlie this method . -
pubmed
Characterization of the folding pathway of
recombinant human macrophage - colony stimulating - factor beta (
rhM - CSF beta ) by
bis - cysteinyl modification and mass spectrometry . -
pubmed
Melarsen oxide [
p -( 4,6 - diamino-1,3,5 - triazin-2 - yl ) aminophenylarsonous acid (
MEL ) ] , which selectively bridges spatially neighboring
bis - cysteinyl residues in ( reduced ) proteins , was used to trap folding intermediates chemically during 1 ) time - dependent renaturation of
recombinant human macrophage colony - stimulating factor (
rhM - CSF ) ; by redox refolding in vitro ; 2 ) reductive unfolding in the presence of the trapping reagent ; and 3 ) denaturing unfolding reactions in
urea and
guanidinium hydrochloride . -
pubmed
In all folding and unfolding reactions a characteristic dimeric intermediate with two attached
melarsen oxide (
MEL ) groups was observed , suggesting that these
rhM - CSF beta species were important refolding intermediates . -
pubmed
Cysteine residues
C7 and C90 were minor modification sites . -
pubmed
The mass spectrometric results from the in vitro folding reactions of
rhM - CSF beta are in agreement with intrinsic
tryptophan fluorescence measurements and are consistent with the folding pathway that starts with a fully reduced monomer ( R ) , includes partially folded monomeric intermediates ( M ) and dimeric intermediates ( D ) , and yields a final product with the native tertiary structure ( N ): 2R = = & gt ; 2 M = = & gt ; D = = & gt ; N. Our results show that selective chemical trapping of
bis - thiol groups of
proteins with
MEL permits study of folding pathways by mass spectrometric structure characterization of intermediates with otherwise transient conformations . -
pubmed
The
topoisomerase 1 inhibitor ,
topotecan , has shown activity in an initial study . -
pubmed
This study was therefore designed to determine whether the specific
thromboxane A2 ( TP ) receptor antagonist ,
GR32191B , is effective in inhibition of
prostanoid or
nonprostanoid receptors in the RA . -
pubmed
Two effects of
GR32191B were tested : ( 1 ) the relaxation induced by
GR32191B in the RA precontracted with the
TP receptor agonists
U46619 and
PGF2alpha or
nonprostanoid vasoconstrictors (
noradrenaline [ NA ] ,
angiotensin II [
AII ] , and
K + ) and ( 2 ) the inhibitory effect of
GR32191B on
TP receptor agonists and
nonprostanoid vasoconstrictors . -
pubmed
RESULTS : In
U46619 ( 10 nm , n=7 ) and
PGF2alpha ( 1 microm , n=7 ) precontracted RA ,
GR32191B induced 100 % relaxation ( 10 - 100 microm ) but not after precontraction with
nonprostanoid stimuli ( 5.8 % for K+ , 25 mm , n=6 , 24.4 % for NA , 3 microm , n=8 , and 53.2 % for
AII , 3 nm , n=5 ) ( P<0.001 ) . -
pubmed
However ,
GR32191B at 3 microm did not significantly inhibit the contraction induced by either NA ,
AII , or K+ . -
pubmed
Biphenotypic B / macrophage cells express
COX-1 and up - regulate
COX-2 expression and
prostaglandin E ( 2 ) production in response to pro - inflammatory signals . -
pubmed
A potential role for B / macrophage cells in
inflammation was shown by their ability to express
prostaglandin H synthase-1 (
COX-1 ) and
prostaglandin H synthase-2 (
COX-2 ) and by their production of
prostaglandin ( PG ) E ( 2 ) . -
pubmed
In contrast ,
COX-2 and the
prostanoids PGE ( 2 ) ,
PGF ( 2alpha ) and
PGD ( 2 ) are highly inducible in B/ macrophage cells upon stimulation with lipopolysaccharide ,
CD40 ligand , or via engagement of
surface IgM , supporting a role for these cells in
inflammation . -
pubmed
PGD ( 2 ) and its metabolites are of interest because they activate the nuclear receptor
PPARgamma that regulates lipid metabolism . -
pubmed
Since
PGE ( 2 ) blunts
IL-12 production , its synthesis by B / macrophage cells may shift the balance of an immune response towards Th2 and humoral immunity . -
pubmed
Electron microscopic evaluation of layers Ia and Ib revealed that
mGluR4a was localized in synaptic terminals in layers Ia and Ib . -
pubmed
Mutation of
Tyr(191 ) of
MMP-1 to
Thr , the corresponding residue in
MMP-3 , reduced collagenolytic activity about 5 - fold . -
pubmed
Low density lipoprotein (
LDL ) particles are the major
cholesterol carriers in circulation and their physiological function is to carry
cholesterol to the cells . -
pubmed
A new molecular interaction model for the beta - sheet structure and
phosphatidylcholine headgroups is introduced and an overall view of the tertiary structure of
apolipoprotein B-100 in the
LDL particles is presented . -
pubmed
Results revealed that 8.1
AH is associated with a high in vivo and in vitro production of
TNF - alpha , which in turn seems responsible for increased serum levels of
sELAM-1 ,
cortisol , and
IL-10 . -
pubmed
Thus , serum values seem to be the result of the effects of increased serum levels of
TNF - alpha and
cortisol . -
pubmed
Signal transduction by the
BvgAS system is characterized by a complex
His - Asp - His - Asp phosphorelay . -
pubmed
Ether extracts of
lactic acid bacteria were analyzed for prevention of the oxidation of erythrocyte membrane and
human low - density lipoprotein in vivo . -
pubmed
A
calcium sensor in the
sodium channel modulates cardiac excitability . -
pubmed
Calcium ions (
Ca2 + ) have a fundamental role in the coupling of cardiac myocyte excitation and contraction , yet mechanisms whereby intracellular
Ca2 + may directly modulate
Na channel function have yet to be identified . -
pubmed
Mutations targeted to the IQ domain disrupted
CaM binding and eliminated
Ca2 +/CaM - dependent slow inactivation , whereas the gating effects of
Ca2 +/CaM were restored by intracellular application of a peptide modelled after the IQ domain . -
pubmed
A naturally occurring mutation ( A1924 T ) in the IQ domain altered
hH1 function in a manner characteristic of the
Brugada arrhythmia syndrome , but at the same time inhibited slow inactivation induced by
Ca2 +/CaM , yielding a clinically benign (
arrhythmia free ) phenotype . -
pubmed
The
beta - lactam resistance was due to production of
beta - lactamases and
ceftazidime proved to be stronger
beta - lactamase inductor than
ceftriaxone . -
pubmed
This case suggests that
interferon - alpha plus
cytarabine ocfosfate therapy may be of benefit in the treatment of extramedullary blast crisis of
CML . -
pubmed
ACTH stimulation tests were carried out consecutively by using 250 microg and 1 microg intravenous
ACTH as a bolus injection after an overnight fast , and blood samples were drawn at 0,30 and 60 min . -
pubmed
The
MN - rate was determined using the
cytochalasin B (
cyt B ) block method . -
pubmed
The validity of the test methods was ascertained by positive controls :
mitomycin C (
MMC ) and
bleomycin (
BLM ) were used in experiments without exogenous activation and
cyclophosphamide (
CP ) in experiments with exogenous activation . -
pubmed
OBJECTIVE : To examine the effects of graded doses of
hydrocortisone ( HC ) on
leptin secretion , and determine the effect of fasting . -
pubmed
Blood sampling was performed every 1 to 2 hours for measurement of
leptin ,
cortisol ,
insulin , and
glucose levels . -
pubmed
Separation of capsular polysaccharide
K4 and
defructosylated K4 derived
disaccharides by high - performance capillary electrophoresis and high - performance liquid chromatography . -
pubmed
Following
chondroitinase digestion of
K4 and its derivative , the two
disaccharides ,
DeltaHexAFrc - GalNAc for
K4 and
deltaHexA - GalNAc for
defructosylated K4 , are separated and readily determined within 20 min on an uncoated fused -
silica capillary using normal polarity at 20 kV and detection at 230 nm . -
pubmed
The described methods were used to evaluate the defructosylation process of
K4 under drastic
acid conditions . -
pubmed
Determination of dissociation constants for a heparin - binding domain of
amyloid precursor protein and heparins or
heparan sulfate by affinity capillary electrophoresis . -
pubmed
The 3
cholesterol stones ( case I ) and the 2 brown pigment stones ( case
II ) showed macroscopic homogeneity , respectively . -
pubmed
The 6
cholesterol stones ( case
IV ) were divided into two types in size . -
pubmed
One such mutant ,
hos2 , was shown previously to have an enhanced induction of stress - responsive genes by cold , whereas the expression of these genes under osmotic stress or the phytohormone
absciscic acid (
ABA ) treatments was not affected . -
pubmed
FIERY1 is a general negative regulator that controls cold , osmotic stress , and
ABA signal transduction and possesses
inositol polyphosphate 1 - phosphatase activity . -
pubmed
Interestingly , the
hos2 mutant protein is extremely tolerant to
Li + . -
pubmed
Serotonergic mechanism of the lateral parabrachial nucleus and
relaxin - induced
sodium intake . -
pubmed
In the present study , we investigated the effects of bilateral injections of
methysergide (
serotonergic receptor antagonist ) into the LPBN on intracerebroventricular ( i.c.v . ) -
pubmed
Intracerebroventricular injections of
relaxin ( 500 ng/1 microl ) induced water intake ( 5.1+/-0.7 ml/120 min ) , but not significant 1.8 %
NaCl intake ( 0.5+/-0.4 ml/120 min ) . -
pubmed
Bilateral injections of
methysergide ( 4 microg/0.2 microl ) into the LPBN strongly stimulated
relaxin - induced 1.8 %
NaCl intake ( 34.5+/-10.9 ml/120 min ) and slightly increased water intake ( 10.5+/-4.9 ml/120 min ) . -
pubmed
relaxin combined with LPBN
methysergide on 1.8 %
NaCl intake ( 0.5+/-0.4 ml/120 min ) . -
pubmed
Losartan ( 50 microg/1 microl ) partially reduced the effects of
relaxin . -
pubmed
The results show that central
relaxin interacting with central angiotensinergic mechanisms induces
NaCl intake after the blockade of LPBN serotonergic mechanisms . -
pubmed
New evidence that both
T - type calcium channels and
GABAA channels are responsible for the potent peripheral analgesic effects of 5alpha - reduced neuroactive
steroids . -
pubmed
Neurosteroids are potent blockers of
neuronal low - voltage activated ( T - type ) Ca ( 2 + ) channels and potentiators of
GABA ( A ) ligand - gated channels , but their effects in peripheral
pain pathways have not been studied previously . -
pubmed
Analgesic effects of
ALPX were incompletely antagonized by co - injections of the
GABA ( A ) receptor antagonist
bicuculline . -
pubmed
However ,
( 3beta,5alpha,17beta )- 17 - hydroxyestrane-3 - carbonitrile (
ECN ) ) , which has effects on T - type channels but not on
GABA ( A ) receptors , also induced potent enantioselective peripheral anti - nociception . -
pubmed
Guanylyl cyclase C and accumulation of
cGMP induced by bacterial heat - stable enterotoxins (
STs ) promote
colon cancer cell cytostasis , serving as a
tumor suppressor in intestine . -
pubmed
Conversely , capacitative
calcium entry through
store - operated calcium channels ( SOCs ) is a key signaling mechanism that promotes
colon cancer cell proliferation . -
pubmed
Elimination of capacitative
calcium entry employing
2 - aminoethoxydiphenylborate (
2 - APB ) , a selective inhibitor of
SOCs , potentiated cytostasis induced by ST . -
pubmed
Opposition of ST - induced cytostasis by capacitative
calcium entry reflects reciprocal inhibition of
guanylyl cyclase C signaling . -
pubmed
Calcium entry through
SOCs induced by the
calcium - ATPase inhibitor
thapsigargin or the receptor agonists
UTP or
carbachol inhibited
guanylyl cyclase C - dependent
cGMP accumulation . -
pubmed
Moreover , regulation by capacitative
calcium entry reflected ligand - dependent sensitization of
guanylyl cyclase C to inhibition by that cation . -
pubmed
Although basal catalytic activity was refractory , ST - stimulated
guanylyl cyclase C was inhibited by
calcium , which antagonized binding of
magnesium to allosteric sites required for receptor - effector coupling . -
pubmed
These observations demonstrate that reciprocal regulation of
guanylyl cyclase C signaling by capacitative
calcium entry through SOCs represents one limb of a coordinated mechanism balancing
colon cancer cell proliferation and cytostasis . -
pubmed
They suggest that combining
guanylyl cyclase C agonists and
SOC inhibitors offers a novel paradigm for
cGMP - directed therapy and prevention for colorectal
tumors . -
pubmed
CONCLUSIONS : This article represents the first prospective , multi - institutional study of HRQOL in men treated with
PB and demonstrates that patients undergoing
PB have a very high overall HRQOL . -
pubmed
PAR produced a time- and dose - dependent increase of
BDNF expression in the three hippocampal subfields examined . -
pubmed
On the contrary , the effect of
DMI on
BDNF mRNA was neither dose- nor time - dependent . -
pubmed
The results suggest that
paroxetine , but not
desipramine , enhances synaptic plasticity in the hippocampus by increasing
BDNF mRNA expression , which determines a later AMPAR subunit trafficking to synaptic membranes . -
pubmed
Others report that
carbonic anhydrase II (
CA II ) binds to the C termini of the anion exchanger
AE1 and the electrogenic
Na /
HCO3 cotransporter
NBCe1 - A , enhancing transport . -
pubmed
After injecting oocytes with
NBCe1 - A cRNA ( Day 0 ) , we measured NBC current ( I(NBC ) ) by two - electrode voltage
clamp ( Day 3 ) , injected
CA II protein +
Tris or just
Tris ( Day 3 ) , measured I(NBC ) or the initial rate at which the intracellular pH fell ( dpH(i)/dt ) upon applying 5 %
CO2 ( Day 4 ) , exposed oocytes to the permeant CA inhibitor
ethoxzolamide (
EZA ) , and measured I(NBC ) or dpH(i)/dt ( Day 4 ) . -
pubmed
Because dpH(i)/dt was greater in
CA II than
Tris oocytes , and
EZA eliminated the difference , injected
CA II was functional . -
pubmed
Moreover ,
EZA had identical effects in
CA II versus
Tris oocytes . -
pubmed
dpH(i)/dt was greater in oocytes expressing EGFP - e1 -
CA II versus EGFP - e1 , and
EZA eliminated the difference . -
pubmed
Slope conductances of EGFP - e1 -
CAII versus EGFP - e1 oocytes were indistinguishable , and
EZA had no effect . -
pubmed
Developmental rates of the
IVF - derived ova to blastocysts and the survival rates of the frozen - thawed blastocysts were not different between the
SOF medium ( 16 and 49 % ) and the co - culture system ( 13 and 61 % , respectively ) . -
pubmed
To determine whether
ADCON - L Gel ( ALG ) or Preclude Spinal Membrane (
PSM ) was more effective in preventing
scarring , reducing
pain , and improving quality of life postoperatively . -
pubmed
Previous laboratory and clinical studies have found
ALG and
PSM to be effective , but none compared the two materials . -
pubmed
PSM and
ALG are equally effective in preventing epidural scarring associated with
postdiscectomy syndrome . -
pubmed
When the studies were combined according to similar measurement units , the overall mean decrease in
malondialdehyde (
MDA ) level was -0.3 mM ( 95 % CI , -0.5 to -0.1 mM ; seven studies ) and -0.8 nmol / mg
low - density lipoprotein (
LDL ) ( 95 % CI , -1.3 to -0.4 nmol / mg
LDL ; three studies ) , respectively . -
pubmed
A meta - analysis on the effect of
Excebrane on pre - dialysis levels of oxidized -
LDL could not be performed due to study heterogeneity . -
pubmed
The selective
cannabinoid CB ( 1 ) receptor antagonist ,
rimonabant , has undergone phase III clinical testing as anti -
obesity drug . -
pubmed
In this scenario ,
rimonabant 's anti -
obesity action is accompanied by favorable changes in markers for
insulin resistance ,
C - reactive protein ,
adiponectin ,
tumor necrosis factor alpha (
TNFalpha ) . -
pubmed
A humanised monoclonal antibody to the
HER2 receptor ,
trastuzumab , has demonstrable single - agent activity in metastatic
breast cancer and enhances the antitumour effects of chemotherapy . -
pubmed
The enzyme belongs to the "
transglutaminase superfamily " that contains a putative catalytic
triad of
cysteine ,
histidine , and
aspartic acid . -
pubmed
The C - terminus of
PNGase was recently found to be a novel
carbohydrate - binding domain . -
pubmed
The compound
URB754 was recently identified as a potent inhibitor of the endocannabinoid - deactivating enzyme
monoacylglycerol lipase (
MGL ) by screening of a commercial chemical library . -
pubmed
Based on HPLC /
MS , NMR and EI /
MS analyses , the present paper shows that the
MGL - inhibitory activity attributed to
URB754 is in fact due to a chemical impurity present in the commercial sample , identified as
bis ( methylthio ) mercurane . -
pubmed
Retinoids exert their effects , in part , by binding to
nuclear receptors , thereby altering gene expression . -
pubmed
Manganese superoxide dismutase mRNA , protein , and activity levels increased in a time - dependent manner upon treatment with
ATRA . -
pubmed
Nuclear levels of the
NF - kappaB proteins p50 and
p65 increased within 24 h of
ATRA administration . -
pubmed
Furthermore an increase in DNA binding to a NF - kappaB element occurred within a 342 - bp enhancer ( I2E ) of the
SOD2 gene with 10 microM
ATRA treatment . -
pubmed
Reporter analysis showed that
ATRA - mediated I2E - dependent
luciferase expression was attenuated upon mutation of the NF - kappaB element , suggesting a contribution of this
transcription factor to
retinoid - mediated upregulation of
MnSOD . -
pubmed
These results suggest that signaling events involving
NF - kappaB and
SOD2 may contribute to the effects of
retinoids used in
cancer therapy . -
pubmed
Loss of
ZFPL1 function also impairs cis - Golgi assembly following
brefeldin A washout and slows the rate of cargo trafficking into the Golgi apparatus . -
pubmed
Epo also enhances
nitric oxide (
NO ) bioavailability through
endothelial NO synthase transcription and activation , and exerts antiapoptotic actions through
Bcl-2 and
Bcl - XL . -
pubmed
NO is a powerful vasodilator ,
insulin - sensitizer , inhibitor of
atherothrombosis and apoptosis , and essential for progenitor mobilization . -
pubmed
Furthermore , inhibition of
JNK with a pharmacologic inhibitor or by selective knockdown blunted the efficacy of
CGC-11093 and
bortezomib . -
pubmed
Therefore ,
CGC-11093 enhances the anticancer activity of
bortezomib by augmenting
JNK - mediated apoptosis and blocking angiogenesis . -
pubmed
Glucagon - like peptide-1 (
GLP-1 ) , a
30 - amino - acid peptide regulates
glucose homeostasis via control of
insulin and
glucagon secretion and by inhibition of gastric emptying and food intake . -
pubmed
Oxyntomodulin ( OXM ) a
37 - amino - acid peptide also derived from the proglucagon gene , binds to both the
glucagon and
GLP-1 receptor (
GLP-1R ) ; however , a separate
OXM receptor has not yet been identified . -
pubmed
Here we show that OXM , like other
GLP-1R agonists , stimulates
cAMP formation and lowers blood
glucose after both oral and ip
glucose administration , actions that require a functional
GLP-1R. -
pubmed
Unexpectedly , OXM , but not the
GLP-1R agonist exendin-4 , increased plasma levels of
insulin after oral
glucose administration . -
pubmed
Biochemical studies show that this molecule inhibits
NAD (+)- dependent DNA ligases , but not
ATP - dependent enzymes . -
pubmed
The molecule inhibits
NAD (+)- dependent DNA ligases competitively with respect to
NAD(+ ) and specifically inhibits enzyme adenylation , but not DNA adenylation or ligation . -
pubmed
Labeling studies establish that this molecule inhibits the incorporation of
thymidine into DNA and that overexpression of
DNA ligase in the cell abolishes this inhibition . -
pubmed
By terminal uridine deoxynucleotidyl transferase
dUTP nick end labeling and flow cytometric analysis , higher apoptotic activity and a remarkable increase in sub - G(1 ) cell population were observed in transfected cells , suggesting that
FSTL1 induced apoptosis in
cancer cells . -
pubmed
Moreover , in - cell NMR spectroscopy demonstrates that
ubiquitin has much higher
hydrogen exchange rates in the intracellular environment , possibly due to multiple interactions with endogenous proteins . -
pubmed
In general ,
COOH - SWNT adsorb to the greatest extent , followed by
OH - SWNT and
P2 - SWNT . -
pubmed
Colibactin acts as a
cyclomodulin and blocks the eukaryotic cell cycle . -
pubmed
Seven conformers of
L - threonine in the gas phase : a LA -
MB - FTMW study . -
pubmed
Rotational spectroscopy in combination with molecular beams and laser ablation ( laser - ablation molecular - beam Fourier transform microwave ( LA -
MB - FTMW ) spectroscopy ) has proved to be successful in characterizing the conformers of natural
amino acids . -
pubmed
erythrocyte
Cu ,
Zn - Superoxide dismutase (
SOD ) ,
Glutathione peroxidase (
GSH - Px ) and
catalase . -
pubmed
In this study , we sought to clarify the nature of this abnormality by combining proton magnetic resonance spectroscopy (
1H -
MRS ) with functional magnetic resonance imaging ( fMRI ) at 3T. -
pubmed
VO(2)AT ,
VO ( 2 )Peak , Load
AT , Load peak and VE / VCO(2 ) slope were measured and compared . -
pubmed
( 2 ) None of VO(2)AT ,
VO ( 2 )Peak , Load
AT , Load peak and VE / VCO(2 ) slope is correlated with LVEF ; VO(2)AT , VO(2)Peak , Load
AT and Load peak all correlate negatively with the higher NYHA grade ; VE / VCO(2 ) slope correlates positively with a higher NYHA grade ; None of VO(2)AT ,
VO ( 2 )Peak , Load
AT , Load peak or VE / VCO(2 ) slope correlates with LVMI . -
pubmed
Reactions of laser - ablated U atoms with
P ( 4 ) molecules upon codeposition in excess
argon gave weak new infrared absorptions at 504 , 483 , and 426 cm(-1 ) , which are best identified as binary
uranium phosphide UP ( 4 ) species based on extensive B3LYP , BPW91 , and
PBE density functional and CASSCF / CASPT2 wave function based calculations . -
pubmed
Synthesis of core 2 branched O - glycans decorated by
sLe ( X ) is dependent on
C2GnT1 function and thus we determined enzyme activity of
C2GnT1 . -
pubmed
Gene knock - down of
C2GnT1 in colon and
hepatic carcinoma cells using short hairpin RNAs ( shRNA ) resulted in a 40 - 90 % decrease in
C2 - O - sLe ( X ) and a 30 - 50 % decrease in
E - selectin binding compared to control cells . -
pubmed
Two - dimensional differential in - gel ( DIGE ) analysis identified 10 differentially expressed proteins between
CHOP - sensitive and -resistant
DLBCL cells that play roles in glycolysis (
triosephosphate isomerase-1 ,
enolase-1 ) , cytoskeletal structure (
ezrin ,
vimentin ,
tubulin - specific chaperone B ) ,
purine biosynthesis (
serine hydroxymethyltransferase ) ,
calcium binding (
sorcin ) , and apoptosis (
p53 ,
14 - 3 - 3zeta ,
Akt ) . -
pubmed
Knockdown of
vimentin by siRNA or
withaferin A repressed the invasiveness of
CHOP - resistant cells in
collagen matrices . -
pubmed
More than 100 mg Nkg(dry soil(-1 ) ) of
NH4 + addition resulted in the reduction of type
II / type I MOB ratios and an obvious evolution of type
II MOB communities , while less than 100 mg
Nkg(dry soil(-1 ) ) of
NH4 + addition induced nearly no change of methanotrophic community compositions . -
pubmed
RESULTS : With IC-1.23 and IC-1.1 , the highest rates of aerosol production were achieved using FC75 ( 2.27 + -0.18 and 0.76 + -0.01 , respectively ) followed by
PFOB ( 1.74 + -0.06 and 0.56 + -0.04 ) ,
PFD ( 0.82 + -0.01 and 0.21 + -0.01 ) , and surfactant ( 0.42 + -0.05 and 0.092 + -0.01 ) . -
pubmed
Changes in
PIP and RR directly influence the percentage of surfactant and
perfluorocarbon recovered . -
pubmed
We found that VVC- and MVC - selected
amino acid changes map to different V3 locations and involve residues that interact with the
CCR5 NT in different ways . -
pubmed
These effects of
DnaD were independent of the ability of
DnaA to bind or hydrolyze
ATP . -
pubmed
We suggest that organisms in which
DnaA has a high rate of
nucleotide exchange predominantly regulate the DNA binding activity of
DnaA and that those with low rates of exchange regulate hydrolysis and exchange . -
pubmed
This report was the first to depict a PET /
CT scan of a liver IMT that revealed an inhomogeneous , intense
( fluorine 18 )- fluoro-2 - deoxy - D - glucose uptake . -
pubmed
gamma - Hydroxybutyric acid induces actions via the
GABAB receptor in arousal and motor control - related nuclei : implications for therapeutic actions in
behavioral state disorders . -
pubmed
In addition , we recorded
GHB - induced membrane responses using whole cell , patch
clamp electrophysiology of immunohistochemically - identified principal neurons within these nuclei . -
pubmed
GHB induced
GABAB receptor - mediated rises in
calcium in neurons of the LDT and the DR . -
pubmed
GHB induced
GABAB receptor antagonist - sensitive outward currents / hyperpolarizations in immunohistochemically - identified cholinergic LDT and serotonergic DR neurons . -
pubmed
Finally ,
NCS-382 and
HOCPCA , a reported antagonist and agonist specific to activity at the putative
GHB receptor , respectively , with no demonstrated binding at the
GABAB receptor , failed to block
GHB - induced effects or elicit any discernible electrophysiological action when applied alone , indicating a lack of involvement of a
GHB receptor in mediating
GHB actions . -
pubmed
total
carbon (
TC ) & gt;13 g kg(-1 ) sediment ,
NH4 (+)-N & gt;144 mg kg(-1 ) sediment ) , while Group 1.1a- and Group 1.1a - associated sequences were dominant in sediments with opposite conditions ( e.g. -
pubmed
TC & lt;4 g kg(-1 ) sediment ,
NH4 (+)-N & lt;93 mg kg(-1 ) sediment ) . -
pubmed
The discovery that
Rett syndrome (
RTT ) is caused by mutation of the
methyl -
CpG - binding - protein
MeCP2 provided a major breakthrough in understanding the
neurodevelopmental disorder and accelerated
MeCP2 research . -
pubmed
PI3K /
AKT /
mTOR signal pathway is an important intracellular signal transduction pathway with a significant role in cell proliferation , growth , survival , vesicle trafficking ,
glucose transport , and cytoskeletal organization . -
pubmed
Specifically , we show that the 40Love /
Samba carboxy - terminal domain ( GRD ) enables nucleocytoplasmic shuttling behavior . -
pubmed
The ability of the
protein to stimulate
oxygen evolution in inside - out
thylakoids was preserved throughout the various fractionation steps . -
pubmed
We report here that
U - II produces an increase in cytosolic
Ca ( 2 + ) concentration in retrogradely labeled cardiac vagal neurons of nucleus ambiguus via two pathways : ( i )
Ca ( 2 + ) release from the endoplasmic reticulum via
inositol 1,4,5 - trisphosphate receptor ; and ( ii )
Ca ( 2 + ) influx through
P / Q - type Ca(2 + ) channels . -
pubmed
For hybrid
strain zygotes ( B6CBF1 ) communal culture in KSOM media supplemented with
amino acids ,
albumin and
Paf under 5 %
O2 resulted in complete rescue of their rate of accumulation of cells and blastocyst formation . -
pubmed
Apoptosis was detected by flow cytometry using
annexin V and
propidium iodide . -
pubmed
RESULTS :
Manumycin treatment resulted in significant decreases in the viabilities of the two
prostate cancer cell lines in a dose - dependent manner through apoptosis , and this apoptosis involved
caspase-9 activation . -
pubmed
A specific inhibitor of
caspase-9 protected cells from
caspase-3 activation , apoptosis , and
cytotoxicity induced by
manumycin . -
pubmed
We also found that
manumycin downregulated
Bcl-2 expression and upregulated
Bax expression . -
pubmed
CONCLUSION : Our data suggest that
manumycin induces apoptosis in
prostate cancer cells through regulation of the
Bcl-2 family involving
caspase-9 activation . -
pubmed
A cation - anion configuration , whereby
BF4 (- ) interacts with the
C2 mpy cation from the side of the ring , is the most likely structure in the low - temperature phase
IV region . -
pubmed
In summary , this study shows that codon optimization strongly increases
hNIS protein levels and
radioiodine uptake , thus supporting opt -
hNIS as a more sensitive reporter and efficient therapeutic gene . -
pubmed
Moreover , the presence of polyanionic cellulose ( PAC ) further improved the rheological and filtration performances of
CNC / BT - WDFs , suggesting a synergistic effect between
PAC and CNCs . -
pubmed
Studies examining the role of
aspirin and
HMG - CoA reductase inhibitors ( statins ) have shown important clinical differences in men versus women in the preventive realm . -
pubmed
Histamine activates
p38 MAP kinase and alters local lamellipodia dynamics , reducing endothelial barrier integrity and eliciting central movement of
actin fibers . -
pubmed
We tested the hypothesis that the barrier - disrupting agent
histamine would reduce local lamellipodia protrusions and investigated the potential involvement of
p38 mitogen - activated protein ( MAP ) kinase activation and
actin stress fiber formation . -
pubmed
The
p38 MAP kinase inhibitor
SB203580 attenuated the
histamine - induced decreases in barrier function and lamellipodia protrusion frequency . -
pubmed
SB203580 also inhibited the
histamine - induced decreases in withdrawal distance and velocity , and the subsequent
actin fiber migration . -
pubmed
These data suggest that
histamine can reduce local lamellipodia protrusion activity through activation of
p38 MAP kinase . -
pubmed
Likewise , in the
TNFalpha - induced
insulin - resistant 3T3 - L1 adipocyte model ,
BAPN prevented the downregulation of
adiponectin and
GLUT4 and the increase in
SOCS3 levels , and consequently normalised
insulin - stimulated
glucose uptake . -
pubmed
We compared
steroid dependency (
relapse & lt;3 months of tapering first course
CS or inability to wean & lt;10 mg
prednisolone ) , need for IFX , linear growth , and surgical resections over the first 2 years . -
pubmed
Synthesis of new
4 - phenylpyrimidine-2 ( 1H )- thiones and their potency to inhibit
COX-1 and
COX-2 . -
pubmed
The objective of the present work was to evaluate physical properties , transport and in vitro modulator effects on
NF - kappaB of
poly ( lactide - co - glycolide ) (
PLGA ) nanoparticles loaded with
lupeol . -
pubmed
C4'-oxidized (
C4 - AP ) and C5'-oxidized abasic sites ( DOB ) that are produced following abstraction of a
hydrogen atom from the DNA backbone reversibly form cross - links selectively with dA opposite a 3'-adjacent
nucleotide , despite the comparable proximity of an opposing dA. -
pubmed
A previous report on
UvrABC incision of DNA substrates containing stabilized analogues of the ICLs derived from
C4 - AP and
DOB also indicated that the latter is repaired more readily by
nucleotide excision repair [ Ghosh , S. , and Greenberg , M. M. ( 2014 ) Biochemistry 53 , 5958 - 5965 ] . -
pubmed
In addition , the preferred
DOB analogue is more reactive than the respective
C4 - AP ICL , which is consistent with its more efficient incision by
UvrABC . -
pubmed
To further corroborate an extrapineal secretion of
melatonin , the presence of the two key enzymes involved in
melatonin synthesis ,
arylalkylamine - N - acetyltransferase (
AANAT ) and
N - acetylserotonin - O - methyltransferase (
ASMT ) was analyzed by RT - PCR , q - PCR and Western - blot in ram testes , epididymis , and accessory glands . -
pubmed
miR-155 Drives Metabolic Reprogramming of ER+
Breast Cancer Cells Following Long - Term
Estrogen Deprivation and Predicts Clinical Response to
Aromatase Inhibitors . -
pubmed
Targeting the glycolysis - priming enzyme
hexokinase-2 (
HK2 ) in combination with the AI ,
letrozole , synergistically reduced cell viability in AI - sensitive models . -
pubmed
Both
CSPs encoded an N - terminal signal peptide , four conserved
cysteines , an OS - D superfamily domain , a 2Fe-2S ferredoxin domain , an anaphylatoxin domain and an
EGF - like domain . -
pubmed
Part 3 :
Notch - sparing
gamma - secretase inhibitors :
SAR studies of
2 - substituted aminopyridopyrimidinones . -
pubmed
In search for novel lead compounds as
gamma - secretase inhibitors , analogs of
aminopyrido [ 2,3 - d ] pyrimidin-7 - ones ( I ) were synthesized and evaluated for inhibitory effects on
amyloid - beta - peptide production and cleavage of the
Notch1 receptor mediated by
gamma - secretase . -
pubmed
Selected
pyridopyrimidines , such as 1 , 8 , 9 , 10 , 11 and 16 are
gamma - secretase inhibitors that did not have an effect on
Notch1 receptor processing . -
pubmed
Adiponectin Attenuates
Angiotensin II - Induced Vascular Smooth Muscle Cell Remodeling through
Nitric Oxide and the
RhoA /
ROCK Pathway . -
pubmed
We investigated whether
nitric oxide (
NO ) , the
RhoA /
ROCK pathway ,
actin cytoskeleton remodeling , and reactive
oxygen species ( ROS ) mediate the anti -
hypertrophic effect of
APN . -
pubmed
Ang II - induced protein synthesis was attenuated by pre - treatment with
APN ,
NO donor
S - nitroso - N - acetylpenicillamine (
SNAP ) , or
cGMP . -
pubmed
The
hypertrophic response to
Ang II was associated with a significant increase in
RhoA activation and vascular force production , which were prevented by
APN and
SNAP . -
pubmed
NO was also associated with inhibition of
Ang II - induced phosphorylation of
cofilin . -
pubmed
Ang II - induced ROS formation and upregulation of
p22 ( phox ) mRNA expression were inhibited by
APN and
NO . -
pubmed
CONCLUSION : Our results suggest that the anti - hypertrophic effects of
APN are due , in part , to
NO - dependent inhibition of the
RhoA /
ROCK pathway and ROS formation . -
pubmed
We also observed weak emission from the
Er ( iii ) cation in the UV region for the first time in a
Ln -
MOF based material . -
pubmed
Though this chapter covers a range of different peptides and protein , if focuses specifically on the opportunities afforded by the de novo design of coiled
coils , miniature protein scaffolds , and the development on
lanthanide - binding sites into these architectures . -
pubmed
DISCUSSION : Recurrent elevations in
cortisol in response to
hypoglycemia may be the underlying pathology leading to suppression of
gonadotropin - releasing hormone (
GnRH ) release . -
pubmed
Oleanolic acid ( OA ) regulates
inflammation and cellular dedifferentiation of chondrocytes via
MAPK signaling pathways . -
pubmed
Inhibition of
p38 with
SB203580 in OA - treated cells rescued the expression of
type II collagen and suppressed the expression of
COX-2 . -
pubmed
However ,
ERK inhibition with
PD98059 accelerated the OA - induced inflammatory responses . -
pubmed
Film characterization with , among others , Fourier transform infrared ( FT - IR ) spectroscopy , X - ray diffraction ( XRD ) , and atomic force microscopy (
AFM ) attests to successful grafting of the polymers to the
laponite nanoparticles . -
pubmed
In particular , evidence of basal plane expansion of
laponite with increasing
silane concentration are obtained using XRD , while patent morphological changes are revealed with
AFM . -
pubmed
While
LP -
PEG - silane is easily applied to a surface from a precursor solution via a dip coating procedure
LP -
APTMS - OEGMA requires lots more chemicals , a thorough control of reaction parameters , and longer reaction time in order to generate films with the desirable properties . -
pubmed
to surfaces is reduced by approximately 85 % in the case of
LP -
PEG - silane and up to 92 % in the case of
LP - APTMS - POEGMA , in comparison to the control surfaces . -
pubmed
We developed a paradigm that allows us to investigate the neuropharmacology by which previously neutral stimuli acquire the capacity to function as CSs and elicit ( intravenous )
cocaine conditioned approach and used this paradigm to test the role of
NMDA receptor stimulation in the acquisition of
cocaine conditioned approach . -
pubmed
Blockade of
NMDA receptors significantly impaired the acquisition of
cocaine - conditioned approach as indicated by the emission of significantly fewer nose pokes and significantly longer latencies to nose poke during
CS presentations . -
pubmed
These results suggest that
NMDA receptor stimulation plays an important role in the acquisition of reward - related conditioned responses driven by intravenous
cocaine - associated CSs . -
pubmed
When
polyoxin AL , a chitin - synthesis - inhibitor , was added to the diet containing MLX56 family proteins , the toxicity of
MLX56 family proteins disappeared and PM became thinner and fragmented . -
pubmed
ALA - mediated biphasic downregulation of
alpha-7nAchR /
HIF-1alpha along with mitochondrial stress modulation strategy in mammary gland chemoprevention . -
pubmed
The efficacy of
ALA was scrutinized in vivo and in vitro using various experiments like hemodynamic studies , morphological analysis , antioxidants parameters , immunoblotting , and quantitative reverse transcription
polymerase chain reaction . -
pubmed
ALA treatment has also increased the level of synaptic
acetylcholine and
acetylcholine esterase with a significant decrease in lipid content . -
pubmed
It was concluded that
ALA persuaded the mitochondrial stress , activation of downstream cholinergic anti - inflammatory markers , and favorable regulation of
hypoxia microenvironment through inhibition of
fatty acid synthase and
sterol regulatory element - binding protein . -
pubmed
Postoperative factors of prognostic importance included hormone - related symptoms , stage
IV disease , raised levels of
5 - HIAA , insufficiency of the tricuspid valve , secondary surgery not being macroscopically radical and a
second malignancy . -
pubmed
We performed fluorescent microscopy , Xpert
MTB /
RIF ( Xpert ) , Lowenstein - Jensen culture ,
human immunodeficiency virus , and random blood
sugar testing on recruited patients . -
pubmed
It was found that
lactic acid (
Lac ) pretreatment combined with
choline chloride /
Lac system could not only selectively convert the hemicellulose to
xylo - oligosaccharides ( XOS ) , which account for 89.7 % of total
xylose in prehydrolysate ( the functional XOS (
DP & lt ; 5 ) took up about 35 % ) , but also significantly promote the
glucose release ( 33.2 g/100 g material ) and well lignin separation ( representing 40.9 % in whole process ) , which better than the single organic pretreatment at higher modified severity index (
SI ) . -
pubmed
One new
5 / 6 bicyclic sesquiterpene and its esterified derivative were characterised by
GC -
MS and 1D and 2D NMR spectroscopy . -
pubmed
Raw PS flours were characterized by greater
tannin and kafirin contents when compared to WS , and , after cooking , PS flours had greater resistant starch ( from 4.2 to 21.4 g /100 g dry matter ) , and lower starch hydrolysis index (
HI ) with respect to cooked WS . -
pubmed
In addition , kafirin and total
tannins content ( on raw ingredients ) along with the
anthocyanin profiles ( on cooked samples ) were negative correlated with
HI . -
pubmed
Regulation of the
SIRT1 signaling pathway in
NMDA - induced
Excitotoxicity . -
pubmed
Silent Information Regulator 1 (
SIRT1 ) , an
NAD+-dependent deacetylase , contributes to the neuroprotective effect . -
pubmed
It is well known that
N - methyl - D - aspartate ( NMDA ) receptor activation induces
calcium influx which then activates
PKC , and
SIRT1 is a mRNA target for
HuR protein . -
pubmed
We hypothesize that
Ca2 + -PKC -
HuR -
SIRT1 pathway modulates
SIRT1 function . -
pubmed
The results showed that : ( 1 )
SIRT1 levels were downregulated in
NMDA model ; ( 2 )
NMDA induced an increase in
serine phosphorylation of
HuR , while inhibition of
serine phosphorylation of
HuR increased
SIRT1 levels , promoting cell survival ; ( 3 )
PKC inhibitor (
Go 6976 ) reversed
NMDA insults and also suppressed
serine phosphorylation of
HuR ; ( 4 )
1,2 - bis ( 2 - aminophenoxy ) ethane - N , N , N ' , N '- tetraacetic acid (
BAPTA - AM ) , an intracellular
calcium chelator , fully reversed
NMDA insults and also inhibited
PKC activity evoked by
NMDA . -
pubmed
These results indicate that intracellular elevated
Ca2 + activates
PKC , which phosphorylates
HuR and then promotes
SIRT1 mRNA decay and subsequent neuronal
death in
NMDA model . -
pubmed
Therefore , the study suggests that inhibition of
Ca2 + -PKC -
HuR -
SIRT1 pathway could be an effective strategy for preventing certain
neurological diseases related to
NMDA excitotoxicity . -
pubmed
The results showed that , in contrast to the control group ( SC ) , the
BITC - treated group ( SQ_
BITC ) had 197 differentially expressed genes (
DEGs ) , of which 115 were downregulated and 82 were upregulated . -
pubmed
TRIM21 and
PHLDA3 negatively regulate the crosstalk between the
PI3K /
AKT pathway and
PPP metabolism . -
pubmed
Here , we demonstrate the important reciprocal crosstalk between the
PI3K /
AKT signal and
pentose phosphate pathway ( PPP ) branching metabolic pathways . -
pubmed
PPP metabolites , in turn , reinforce
AKT activation and further promote
cancer metabolic reprogramming by blocking the expression of the
AKT inhibitor
PHLDA3 . -
pubmed
Twenty - four patients were treated with
cetuximab with 8/167 ( 4.7 % ) identified as being treated with a
RAS variant ( n = 3 ) or incomplete testing ( n = 5 ) ; thus , not in accordance with guidelines . -
pubmed
Serological tests for serum
creatinine ,
ALT , and
AST were performed . -
pubmed
Thrombosis in
post - transcatheter aortic valve replacement ( TAVR ) patients has been correlated with flow stasis in the neo -
sinus . -
pubmed
The results showed that all
post - TAVR anatomical measurements were significantly different in patients with and without
thrombus , but only
sinus diameter had a linear correlation with
thrombus volume ( r = 0.471 , p = 0.008 ) . -
pubmed
Thus , root anatomy , reactive
oxygen species detection by fluorescence microscopy and , ROS histochemical staining along with the
NADPH oxidase activity were analyzed . -
pubmed
BACKGROUND :
PRAME ( PReferentially expressed
Antigen in
MElanoma ) has shown utility in distinguishing
melanoma from benign
melanocytic lesions , but knowledge of its expression pattern in intermediate melanocytic and spitzoid proliferations is limited . -
pubmed
This study explores the potential to reach a circular economy for
post - consumer Recycled
Polyethylene Terephthalate (
rPET ) packaging and bottles by using it as a Distributed Recycling for Additive Manufacturing ( DRAM ) feedstock . -
pubmed
It was found that AGM and its
enzyme - degraded products , though with different molecular weights , had similar beta - glycosidic bonds and
monosaccharide compositions , exerted similar strength of hypoglycemic effects , and reinstated with a similar extent the disordered gut microbiota and the contents of
SCFAs and endogenous metabolites . -
pubmed
Overall , our observation indicated that
CYP or
MAT could disturb the hormonal balance , and their combination of 0.1 mug / L
CYP +25 mug / L
MAT could significantly enhance the estrogenic effect . -
pubmed
By hemagglutination , hemagglutination inhibition , and immunofluorescence techniques , it was shown that the
anti - VH antiserum detected three sets of antigens in the
VH region , namely idiotypic antigens ,
VH subgroup - specific antigens , and
VH domain-(framework ) specific
antigens . -
pubmed
The release of
TG showed significant correlations with the uptake of
glycerol . -
pubmed
The second extraction step with
4M - guanidinium chloride yielded only bands I and
II . -
pubmed
It is found by means of different methods ( the administration of
35S - heparin , the determination of fibrinolytic activity of blood plasma fractions containing
fibrinogen degradation products and
fibrinogen -- heparin complex , spectral analysis of
fibrinogen -- heparin complex etc . ) -
pubmed
Results obtained all the conclusion that two homeostatic equilibrium of
L - carnitine family components , namely the inter - exchange between
L - carnitine and its
esters catalyzed by
carnitine acyl transferases , and a saturable tubular reabsorption process with differentiated threshold for each component . -
pubmed
omega - CT had no effect on either the electrically paced contractile response that was elicited by direct muscle stimulation or the enhancement of the paced response caused by
beta - adrenoceptor agonist stimulation . -
pubmed
Neomycin also caused insurmountable inhibition of responses elicited by
beta - adrenoceptor agonist stimulation . -
pubmed
In the vas deferens , ileum and atria the inhibitory effects of
omega - CT were long - lasting , whereas the effects of
neomycin could be reversed upon
wash - out . -
pubmed
Although the
PHA - induced mitogen response of lymphocytes from the other three lymphoid compartments remained suppressed throughout the study period , the response to the
mitogens Con - A and PWM generally returned to normal or supranormal levels by the seventh postburn day , Therefore it appears that the effect of a
thermal injury on lymphocyte function varies according to the lymphocyte compartment examined and the
mitogen tested . -
pubmed
Even at an
etodolac concentration of 80 micrograms / ml , DNA synthesis , proteoglycan synthesis , and
type - II collagen synthesis were unchanged . -
pubmed
Collagenase production was also unaffected by
etodolac ( 60 micrograms / ml ) . -
pubmed
The principle of the colorimetric method depends on the determination rate of
p - nitrophenol released on hydrolysis of
N - CBZ - L - leucine - p - nitrophenyl ester at pH 8.0 by
subtilisin , with correction for any nonenzymatic ( spontaneous ) hydrolysis of the substrate . -
pubmed
Thymidine kinases purified from the different sources were similarly sensitive to inhibition by
dTTP or
5 '- AdThd and to deinhibition of the
dTTP - induced regulation of
enzyme activity by
5 '- AdThd . -
pubmed
In none of these cultures was
nucleoside phosphorylase activity detected , as indicated by the inability of the cells to produce
thymine or
iodouracil after exposure to the appropriate
nucleosides . -
pubmed
Also ,
5 '- AdThd did not affect the breakdown of
dTMP by crude preparations of cytosolic
5 '- nucleotidase from the different cells . -
pubmed
We found that intracellular
dTTP pools in the various cell types were substantially high ( 15 - 26 microM ) compared to the sensitivity of
thymidine kinase to inhibition by
dTTP ( IC50 2 - 4 microM ) . -
pubmed
To test the sensitivity of
thymidine kinase ( in situ ) to regulation by
dTTP we investigated : ( a ) the effect of depleting intracellular
dTTP pools with
methotrexate on the uptake of
thymidine (
dThd ) ; and ( b ) the effect of pH on the uptake of
dThd and its perturbation by
5 '- AdThd , since the inhibition of
thymidine kinase activity by
dTTP is known to be pH dependent . -
pubmed
Sodium dodecylsulphate (
SDS ) , a component in
IL-2 excipient , was found to cause a short term breach in the BBB , enabling the penetration of viruses into the brain . -
pubmed
Predicting bone marrow
iron stores in
anemic patients in a community hospital using
ferritin and erythrocyte sedimentation rate . -
pubmed
Forty - three consecutive cases from a community hospital with concomitant bone marrow
iron stain ,
serum ferritin , and erythrocyte sedimentation rate ( ESR ) were reviewed . -
pubmed
A two - dimensional linear graphic relationship between
ferritin and ESR correctly identified six of nine
iron - deficient patients and 32 of 34
iron - present patients . -
pubmed
Lupus anticoagulants represent a subclass of anti -
phospholipid antibodies which are characterized by their ability to prolong the clotting time in in vitro coagulation assays measuring partial
thromboplastin time ( PTT ) ( Thiagarajan , P. , Shapiro , S. S. , and DeMarco , L. ( 1980 ) J. Clin . -
pubmed
Competition experiments , using a modified PTT assay , demonstrated that hexagonal phase
phospholipids , including natural and synthetic forms of
phosphatidylethanolamine , were able to neutralize the lupus anticoagulant activity of all 11
hybridoma antibodies . -
pubmed
Thus , these
antibodies are capable of recognizing
phospholipids on purely structural criteria . -
pubmed
The demonstration that
anti - phospholipid antibodies are able to distinguish between different structural arrangements of
phospholipid may have important implications regarding the immunoregulation of autoimmunity . -
pubmed
Met - enkephalin - like immunoreactivity in neurointermediate pituitary is decreased by
DA receptor stimulation . -
pubmed
Bromocriptine treatment resulted in a dramatic decline of
Met - enkephalin - LI in neurointermediate pituitary which was significant by 3 days of treatment . -
pubmed
The stimulation in RNA synthesis does not result from expansion of the
nucleotide pool but is correlated with increases in
RNA polymerase activity . -
pubmed
Glucose administration ( 3 g / kg , by gavage , a dose sufficient to cause
insulin - mediated reductions in plasma levels of the large neutral
amino acids leucine ,
isoleucine , and
valine ) also elevated brain
phenylalanine and
tyrosine , and enhanced the increments caused by the
aspartame , nearly doubling the rise in brain
phenylalanine . -
pubmed
Norepinephrine and
renin activity in
chronic renal failure . -
pubmed
The significance of PRA was further assessed by
angiotensin blockade with
saralasin . -
pubmed
Lectin binding
strain - specific
carbohydrates on the cell surfaces of Leishmania strains from the Old World . -
pubmed
The applied demetallization procedures ( dialysis against 0.1 M
HCl or 1 M
acetic acid and against 0.1 M
EDTA following by dialysis against 1 M
acetic acid ) had no effect on the stability of the
lectin subunit structure , as proved by ultracentrifugation analysis or thin - layer chromatography on Sephadex G-200 Superfine . -
pubmed
Affinity electrophoresis on
polyacrylamide gel containing immobilized
alpha - D - galactosyl residues and affinity chromatography on
alpha - D - galactosyl derivative of Separon have shown that the interaction of the demetallized
lectin preparations does not differ from that of the native
lectin in alkaline media but is decreased in acidic media . -
pubmed
CKA encodes a protein that is 80 % identical at the
amino acid level to the
human Mel1a melatonin receptor and is thus designated the
chick Mel1a melatonin receptor . -
pubmed
The widespread distribution of
CKA and
CKB mRNA in brain provides a molecular substrate for the profound actions of
melatonin in birds . -
pubmed
An association between
alcohol and
high - density lipoprotein cholesterol was found only in the latest survey and probably reflects the aging of the cohort . -
pubmed
Analysis with multiple linear regression showed that all variables with the exception of
triglycerides ,
HDL cholesterol and height were significantly associated with
systolic blood pressure . -
pubmed
[
Cys5 ,
Cys11 ] Dyn
A1 - 11 - NH2 ( 1 ) and
[ Cys5 , D - Ala8 ,
Cys11 ] Dyn
A1 - 11 - NH2 ( 2 ) are not only highly potent kappa - selective peptides but they also exhibit exceptional selectivity for
kappa receptors in the central ( brain ) vs. the peripheral ( ileum ) systems . -
pubmed
The
chromosome aberration assay ( CA ) in bone marrow cells and the micronucleus test (
MNT ) in both bone marrow and peripheral blood erythrocytes have been carried out for the evaluation of the clastogenic potential of
acephate (
asataf ) in a chick in vivo test system . -
pubmed
Co - infusion with
BQ-123 ( 0.1 mg kg-1 min-1 ) prevented the rise in MAP produced by big
ET-1 and completely blocked the renal response . -
pubmed
Similarly ,
BQ-123 inhibited both the increase in MAP and the decrease in RPF and
GFR produced by
ET-1 . -
pubmed
Big
ET-1 but not
ET-1 , produced a significant increase in water and
sodium excretion . -
pubmed
BQ-123 had no effect on the diuretic and natriuretic response to big
ET-1 consistent with possible
ETB - mediated inhibition of tubular reabsorption . -
pubmed
We have previously shown that at higher doses of
ET-1 ,
BQ-123 was unable to inhibit the renal vasoconstrictor response despite blockade of the pressor response . -
pubmed
We conclude that serotoninergic neurotransmission plays an important part in
post -
stroke pathological crying and that
citalopram is an effective and well - tolerated treatment . -
pubmed
When both extra- and intracellular
Ca2 + were depleted , the activation of
p72syk was still persistent ; in contrast , the deactivation process was completely abrogated even at 6 min after stimulation . -
pubmed
In addition ,
genistein and
herbimycin A , potent
protein - tyrosine - kinase inhibitors , were capable of reducing concanavalin A - evoked
p72syk activation and
Ca2 + mobilization as well as the aggregation and
lysozyme release . -
pubmed
These lines of evidence suggest that
p72syk is activated with concanavalin A in a
Ca ( 2 +)-independent manner , participating in a mechanism of
Ca2 + recruitment , and negatively regulated by a feedback mechanism through
Ca2 + in neutrophils . -
pubmed
Vicinal dihaloalkanes are transformed by
glutathione S - transferase - catalyzed reactions to mutagenic and
nephrotoxic S -( 2 - haloethyl ) glutathione S - conjugates . -
pubmed
The first step is hepatic
glutathione S - conjugate formation followed by cleavage to the corresponding
cysteine S - conjugates , and , after translocation to the kidney , metabolism by renal
cysteine conjugate beta - lyase . -
pubmed
Beta - Lyase - dependent metabolism of
halovinyl cysteine S - conjugates yields electrophilic
thioketenes , whose covalent binding to cellular macromolecules is responsible for the observed
toxicity of the parent compounds . -
pubmed
Finally , hepatic
glutathione conjugate formation with
hydroquinones and
aminophenols yields conjugates that are directed to
gamma - glutamyltransferase - rich tissues , such as the kidney , where they undergo alkylation or redox cycling reactions , or both , that cause organ - selective damage . -
pubmed
Blood
glucose and serum
insulin levels were identical in both sessions . -
pubmed
The rate of conversion of
[ 14C ] arachidonic acid to
[ 14C ] PGE2 (
PGE2 synthase ) was determined in cell sonicates under optimal in vitro conditions . -
pubmed
After a treatment period of 4 h we observed an increase in
PGE2 production in the presence of
mEGF ( 4.7 - fold ) ,
VAN ( 1.8 - fold ) or both agents ( 6.8 - fold ) over control samples . -
pubmed
Similarly , the specific activity of
PGE2 synthase was stimulated maximally after 4 h with
mEGF ( 2.1 - fold ) ,
VAN ( 1.7 - fold ) or with both ( 4.8 - fold ) compared with untreated tissue samples . -
pubmed
We suggest , that
VAN and
mEGF act by a mechanism(s ) that involves de novo protein synthesis or that alters the phosphorylation state of
enzymes that are requisite for the conversion of
arachidonic acid to
PGE2 . -
pubmed
BP was measured with a
mercury sphygmomanometer after a 10 min
rest three times in 5 min . -
pubmed
decreased levels of
parathyroid hormone ,
calcitonin and the transport form of
vitamin D3 . -
pubmed
Parathion (
PA ) is a
phosphorotioate pesticide requiring
P-450 - mediated oxidations to become activated to
paraoxon , or to be metabolised to its less toxic metabolites . -
pubmed
On the other hand ,
sodium arsenite [
As ( III ) ] markedly decreases total hepatic
P-450 content and dependent
monoxygenase activities . -
pubmed
Our aim was to determine the effects of
As ( III ) pretreatment on the acute
toxicity of
PA and its possible relationship with the effects of
As ( III ) on
P-450 - dependent
monooxygenase activities . -
pubmed
As ( III ) pretreatment increased the acute
toxicity of
PA , reducing 38 % its median lethal dose ( LD50 ) from 11.68 to 7.21 mg
PA / kg . -
pubmed
In addition ,
As ( III ) pretreatment further decreased the inhibitory effect of
PA on brain
acetylcholinesterase activity , reducing 33 % the median inhibitory dose ( ID50 ) from 3.44 to 2.31 mg
PA / kg . -
pubmed
As ( III ) decreased the
P-450 content to 87 % of control values , reduced
EROD activity to 74 % and
BROD activity to 41 % ;
PA produced no significant effects on these parameters , whereas the joint administration of
As ( III )+ PA produced effects similar to those of
As ( III ) . -
pubmed
PROD activity was reduced to 36 % of control value by
PA , whereas
As ( III ) alone produced no significant effects . -
pubmed
Stimulation of lipolysis in vitro by the effectors
isoproterenol with or without
adenosine deaminase ,
dibutyryl cAMP with or without
isobutylmethylxanthine , and
forskolin was not altered by ST treatment . -
pubmed
The computational time was short ( approximately 60 s for the prediction of
proteins with 200
amino acid residues ) . -
pubmed
Growth hormone reserve was assessed by at least two stimulation tests (
clonidine ,
L - dopa ,
growth hormone - releasing hormone ) . -
pubmed
The
GH response to
clonidine was significantly less than that found with the other stimuli . -
pubmed
The growth rate in conjunction with the
GH response to
clonidine provides a sensitive measure of
GHD associated with chemotherapy . -
pubmed
We conclude that
C / EBPalpha is required for the
cAMP induction of
PEPCK gene expression in the liver and that
C / EBPbeta can compensate for the loss of
C / EBPalpha if its concentration is induced to appropriate levels . -
pubmed
Plasma
glucose concentrations decreased after 100 and 150 micrograms kg-1
IGF-1 from 3.40 to 2.50 and 2.34 mmol l-1 , respectively ( SED 0.314 , P & lt ; 0.001 ) , but were not significantly altered after lower doses . -
pubmed
The degree of early - phase bronchial responsiveness to allergen ( the cumulated dose of allergen causing a 20 % fall in FEV1 [ PD20allergen ] ) was related to the degree of nonspecific bronchial responsiveness ( the concentration of
histamine causing a 20 % fall in FEV1 [
PC20histamine ] ) and the level of specific
IgE or allergen thresholds as found in skin tests and basophil
histamine release assays . -
pubmed
Weak correlations were found between
PD20allergen and the level of specific
IgE ( r = -0.36 , p = 0.07 ) or allergen thresholds as found in skin tests ( skin prick test , r = 0.36 and p = 0.07 ; intracutaneous test , r = 0.49 and p = 0.01 ) or basophil
histamine release assays ( r = 0.37 , p = 0.08 ) . -
pubmed
Moreover , no significant contribution of these indices of
IgE - mediated
hypersensitivity to the prediction of PD20allergen by multilinear regression models with
PC20histamine was found . -
pubmed
Nitric oxide (
NO ) is a multifunctional molecule that is synthesized by a family of enzymes , namely
nitric oxide synthases (
NOS ) . -
pubmed
NO modulates the expression of
bcl-2 family members ,
p53 ,
interleukin-1 beta - converting enzyme family proteases and the
cytokine receptor Fas . -
pubmed
Therefore ,
NO generated from
NO donors or synthesized by
NOS induces cell
death via apoptosis in a variety of different cell types . -
pubmed
The electronic absorption spectra of these
hemoproteins were similar to those of native
MnP , but absorption maxima were shifted to longer wavelengths in the order of
Deut - rMnP ,
MPP - rMnP , Etio - rMnP ,
DME - rMnP , and rMnP. -
pubmed
All
enzymes contained a high - spin , pentacoordinate
heme iron as evidenced by the characteristic charge transfer bands in the visible region . -
pubmed
The
hemoproteins exhibited reduced catalytic activity while maintaining similar substrate Km values compared to native
MnP. -
pubmed
Compounds I ,
II , and III were obtained for these
hemin - analogue enzymes except for Deut - rMnP. -
pubmed
Histamine plays an important role in bronchoconstriction mediated by
histamine receptors which provoke
bronchial asthma attack . -
pubmed
Glucose - induced thermal stabilization of the native conformation of
GLUT 1 . -
pubmed
It is concluded that the shift to a higher denaturation transition temperature in the presence of
D - glucose is not a result of increased kinetic stability of
GLUT 1 . -
pubmed
In addition , the redox regulation of inactivation , which is typical for
Kv1.4 channels , can be conferred to the inactivation of the deleted constructs by incorporation of an N - terminal
cysteine residue . -
pubmed
Barrier disruption effected by
dioctanoyl ( di - C8 ) phosphatidate was markedly potentiated by the addition of
propranolol , an inhibitor of
endothelial cell " ecto "- phosphatidate phosphohydrolase (
PAP ) , a
lipid phosphate phosphohydrolase (
LPP ) that efficiently hydrolyzes extracellular substrate . -
pubmed
Phosphatidate - induced permeability was attenuated by preincubation of endothelial cells with the
tyrosine kinase inhibitor ,
herbimycin A ( 10 microg / ml ) , and enhanced by the
tyrosine phosphatase inhibitor ,
vanadate ( 100 microM ) , implicating a role for activation of intracellular
tyrosine kinases in the response . -
pubmed
In addition ,
phosphatidate increased the levels of intracellular free
Ca ( 2 + ) in endothelial cells and ligated specific binding sites on endothelial cell plasma membranes , consistent with the presence of a
phosphatidate receptor . -
pubmed
We conclude that
phosphatidate may be a physiologic modulator of endothelial monolayer permeability that exerts its effects by activating a receptor - linked ,
tyrosine kinase - dependent process which results in mobilization of intracellular stored
Ca ( 2 +)and consequent metabolic activation . -
pubmed
GC and
GC /
MS analysis indicated that the cuticular
hydrocarbons with chain lengths & gt;21
carbons were the same as those found in the hemolymph . -
pubmed
Glucose oxidation ( micromol / h per mg protein ; mean ( SEM ) ) on day 7 of
DSS administration was significantly higher than in controls ( 0.06 ( 0.006 ) v 0.027 ( 0.004 ) , p<0.001 ) . -
pubmed
Production of
beta - hydroxybutyrate was decreased and production of
lactate increased in
DSS colitis compared with controls . -
pubmed
Increasing
butyrate concentration from 10 to 80 mM enhanced oxidation in
DSS colitis ( 0.036 ( 0.002 ) to 0.285 ( 0.040 ) , p<0.001 ) , although it continued to remain lower than in controls . -
pubmed
When 1 mM
DSS was added to normal colonocytes in vitro , it did not alter
butyrate oxidation . -
pubmed
Abnormal
butyrate oxidation became apparent only after six days of
DSS administration , at which time histological abnormalities were more widespread . -
pubmed
MRS was employed to measure the level of real - time intestinal
ATP and pH in vivo . -
pubmed
In group C , tissue pH was significantly lower than in other groups during I / R. CONCLUSION :
Leukocytosis induced by
G - CSF exerts a prolonged effect on
ATP during I / R and leukocyte depletion helps protect against the I / R
injury . -
pubmed
Did
nucleotides or
amino acids drive evolutionary conservation of the
WT1 + /-
KTS alternative splice ? -
pubmed
One highly conserved feature of the
Wilms ' tumour suppressor gene ,
WT1 , is the potential , following alternative pre - mRNA splicing , to insert three
amino acids ( KTS ) between the third and fourth zinc fingers . -
pubmed
No sera showed
antibodies that reacted to only
PAH . -
pubmed
An immunocompetition assay demonstrated that the reactivity against
PAH represents a cross - reactivity with
TPH , whereas antibodies against
TPH and
TH are directed towards epitopes unique for the two enzymes . -
pubmed
Two series of fungal metabolites ,
enniatins and
beauvericins , were found to be ubiquitous and potent inhibitors of
ABC transporters . -
pubmed
To study the role of these interactions in DNA transfer and to identify essential
amino acids of
VirB8 , we introduced random mutations in
virB8 by the mutagenic PCR method . -
pubmed
Analysis of the mutant
strains by DNA sequence analysis , Western blot assays , and reconstruction of new point mutations led to the identification of five
amino acid residues that are essential for
VirB8 function . -
pubmed
The substitution of
glycine-78 to
serine ,
serine-87 to leucine ,
alanine-100 to
valine ,
arginine-107 to
proline or
alanine , and
threonine-192 to
methionine led to the loss of
VirB8 activity . -
pubmed
TASK-5 , a new member of the tandem - pore
K (+ ) channel family . -
pubmed
Four
TASKs (
TASK1 - 4 ) identified so far form functional
K (+ ) channels and encode background
K (+ ) channels in various cell types . -
pubmed
TASK-5 shares 51 %
amino acid identity with
TASK-1 and
TASK-3 . -
pubmed
Thus ,
TASK-5 is a new member of the tandem - pore
K (+ ) channel family but does not produce a functional plasma membrane
K (+ ) current by itself . -
pubmed
The physical association between the two enzymes alters the DNA /
topoisomerase II interaction as shown by
pulse - field electrophoresis after incubation of intact nuclei with the specific
topoisomerase II inhibitor
VM-26 . -
pubmed
We previously developed a formula to estimate the individual
carboplatin clearance ( CL ) based on serum
creatinine ( Scr ) determined by an enzymatic assay using
creatinine amidohydrolase . -
pubmed
After subretinal injection , both lenti - VSVG and AAV2/5 transduced the retinal
pigment epithelium (
RPE ) and photoreceptors efficiently whereas transgene expression was restricted to
RPE cells using lenti with the Mokola envelope or AAV2/1 . -
pubmed
The icv infusion of the selective
glucocorticoid receptor antagonist
RU38486 blocked the
hyperphagic effects of twice - daily icv injections of
DEX in both sexes . -
pubmed
Resistant isolates , containing the
tcrB gene , grew on brain heart infusion agar plates containing up to 28 mM
CuSO4 compared to only 4 mM for the susceptible isolates . -
pubmed
The present study was designed to evaluate the link between platelet activation and
GD3 expression and to verify whether this
ganglioside might play a role in modulating signal transduction events . -
pubmed
Our results suggest that following platelet activation ,
GD3 is rapidly expressed on the platelet surface and internalised to the cytoskeleton where it transiently associates first with the
Src family tyrosine kinase Lyn then with the
Fc receptor gamma chain . -
pubmed
These data drive us to speculate that
GD3 might act as second messenger in the activatory cascade , which leads to
CD32 expression and triggers platelet adhesion and spreading to the subendothelial matrix . -
pubmed
For dose - corrected concentrations ( C / D ) and for clearance ( Cl ) we found the coefficient of variation ( CV ) to be approximately 55 % for all variables ( C / D
CIT , C /
D DCIT , the ratio
DCIT to
CIT , and for
Cl CIT ) . -
pubmed
On a population level , signs of a possible saturation of
CYP2D6 associated with increasing
DCIT - to - CIT ratios with increasing daily doses was observed . -
pubmed
Finally , concomitant medication affected the outcome of serum concentrations by a general increase in C / D
CIT and C /
D DCIT but without alteration in the
DCIT - to -
CIT ratio . -
pubmed
BACKGROUND : The association between sympathetic activation and mortality in chronic
heart failure and the favorable effect of beta blocking drugs has raised the possibility of therapeutic efficacy for central sympathetic inhibition with sustained - release (
SR )
moxonidine , an
imidazoline receptor agonist . -
pubmed
Final analysis revealed 54
deaths ( 5.5 % ) in the
moxonidine SR group and 32
deaths ( 3.4 % ) in the placebo group during the active treatment phase . -
pubmed
Hospitalization for
heart failure , acute
myocardial infarction and adverse events were also more frequent in the
moxonidine SR group . -
pubmed
Plasma
norepinephrine was significantly decreased by
moxonidine SR ( -18.8 % from baseline ) vs. placebo ( +6.9 % ) . -
pubmed
Nonetheless , the excess early mortality and morbidity suggest the likelihood of an adverse effect of
moxonidine SR and raise concerns regarding the efficacy of generalized sympathetic inhibition in
heart failure . -
pubmed
Two independent methods have been critically evaluated and applied to the measurement of total
homocysteine in serum and plasma : solid - phase anion extraction ( SPAE ) gas chromatography / mass spectrometry (
GC /
MS ) and
protein precipitation liquid chromatography / tandem mass spectrometry ( LC /
MS /
MS ) . -
pubmed
On the basis of the excellent precision and close agreement of the mass spectrometric (
MS ) methods , the
MS - based methods will be used for certification of a serum - based
SRM for
homocysteine and
folates . -
pubmed
These data suggest that : ( i ) The actions of
zotepine in discrimination assays are similar to , but not identical with , those of
clozapine and
quetiapine ; ( ii ) The differences among the actions of
clozapine ,
quetiapine and
zotepine may be related to either the unique ability of
zotepine to block
noradrenaline ( NA ) uptake , or to its more marked affinity for
D ( 2 ) receptors ; ( iii ) The finding that
zotepine only mimicked
quetiapine up to a level of 54 % was unexpected , since
quetiapine and
clozapine generalise reciprocally and
zotepine generalised fully to ( low dose )
clozapine . -
pubmed
[ Therapeutic use of
ACTH and
cortisone ] . -
pubmed
Paraoxon and
phenylacetate were used as substrate for measuring
PON1 activities and
1 - hexadecyl-2 -[ 3H ] acetyl - glycero-3 - phosphocholine for
platelet - activating factor acetylhydrolase (
PAF - AH ) activity . -
pubmed
PON1 activities correlated positively and significantly with
HDL -
cholesterol ,
HDL2 -
cholesterol ,
HDL3 -
cholesterol ,
HDL - phospholipids ,
apo A - I ,
apo A - II , and
LpA - I :
A - II . -
pubmed
Enzymatic hydrolysis of gelatin layers on used lith film using thermostable
alkaline protease for recovery of
silver and
PET film . -
pubmed
To develop a new efficient and potential industrial enzymatic process for the recovery of
silver and
poly ( ethylene terephthalate ) (
PET ) from used lith film for printing , which has not been recycled at all , enzymatic hydrolysis of gelatin layers on lith film was investigated using the thermostabilized mutant enzyme of the
alkaline protease from alkaliphilic Bacillus sp . -
pubmed
Administration of NKT cell ligands ,
alpha - galactosylceramide (
alpha - GalCer ) resulted in the activation of both
cytokine production and natural killing . -
pubmed
Intracellular staining experiments demonstrated that NKT cells were the earliest source of both
IL-4 and
IFN - gamma production after
alpha - GalCer administration in vivo . -
pubmed
In contrast , isolated
NK1.1+
CD3- classical NK cells exhibited greatly enhanced natural killing activity 6 h after
alpha - GalCer administration . -
pubmed
NKT cells , however , exhibited a strong
cytotoxicity when they were activated and expanded with
alpha - GalCer plus
IL-2 in vitro . -
pubmed
These results indicated that NKT cells act as regulatory cells via production of
cytokines for activation of NK cell - mediated
cytotoxicity in vivo at early phase after
alpha - GalCer administration . -
pubmed
The combination of
HSP70 overexpression in situ and
tumor killing by
thymidine kinase /
ganciclovir treatment , but neither strategy alone , provoked potent systemic antitumor activities after intratumor injection of Ad -
HT . -
pubmed
The epitope of
HMMC-1 was determined by transfection with a panel of
glycosyltransferase cDNAs and by inhibition assays with chemically synthesized
oligosaccharides . -
pubmed
CONCLUSIONS : These results indicate that
HMMC-1 specifically recognizes a novel
O - glycan structure . -
pubmed
Fluorescence and CD spectroscopic analysis of the
alpha - chymotrypsin stabilization by the ionic liquid ,
1 - ethyl-3 - methylimidazolium bis [( trifluoromethyl ) sulfonyl ] amide . -
pubmed
The stability of
alpha - chymotrypsin in the ionic liquid ,
1 - ethyl-3 - methyl - imidizolium bis [( trifluoromethyl ) sulfonyl ] amide (
[ emim ][ NTf2 ] ) , was studied at 30 and 50 degrees C and compared with the stability in other liquid media , such as water , 3 M
sorbitol , and
1 - propanol . -
pubmed
The kinetic analysis of the
enzyme stability pointed to the clear denaturative effect of
1 - propanol , while both 3 M
sorbitol and
[ emim ][ NTf2 ] displayed a strong stabilizing power . -
pubmed
The
[ emim ][ NTf2 ] enhanced both the melting temperature and heat capacity of the
enzyme compared to the other media assayed . -
pubmed
The fluorescence spectra clearly showed the ability of
[ emim ][ NTf2 ] to compact the native structural conformation of
alpha - chymotrypsin , preventing the usual thermal unfolding which occurs in other media . -
pubmed
While the
tyrosine phosphorylation of
STAT3 , which is indicative of its signal - dependent activation , is a common occurrence in
tumors , and appears to play a crucial role in some
malignancies , a variety of new data suggest that it can be dispensable under some circumstances and
STAT3 can participate in transformation through novel and non - canonical mechanisms . -
pubmed
U0126 , a specific inhibitor of
MEK1 / 2 , downregulated
CAIX expression induced by true
hypoxia and cell density . -
pubmed
Mapping of the
U0126 effect revealed that both critical elements within the
CA9 promoter , the
hypoxia response element and the juxtaposed
SP1 - binding
PR1 , were inhibited . -
pubmed
Most importantly , release of
pyridoxal-5 '- phosphate and
protein thermal denaturation were found to occur simultaneously at pH 6.0 in contrast to pH 8.5 where denaturation preceded cofactor 's release by approximately 3 degrees C. To correlate the observed differences in thermal denaturation with structural features , the crystal structure of
phosphoserine aminotransferase was determined at 1.2 and 1.5 A resolution at two different pHs ( 8.5 and 4.6 , respectively ) . -
pubmed
Missense mutations in the
Notch3 gene that create or destroy
cysteine residues , have been found in most cases with a family history of the disease , although a few sporadic cases harbouring
Notch3 mutations have also been described . -
pubmed
SSE for SCs is similar to that described previously for PCs in that it is prevented by
BAPTA and
DAG lipase inhibitors in the recording pipette ; however , unlike in PCs ,
NMDA receptors ( NMDARs ) play an important role in SSE for SCs . -
pubmed
Contrasting to normal CA , normal TA showed lower endothelial
calcium signaling with more concentrated
NF - kappaB in the endothelial nuclei . -
pubmed
Molecular Dynamics simulations in aqueous solution were performed for the
matrix metalloproteinase-8 (
MMP-8 ) free catalytic domain and for its complexes with the
( R )- and ( S )-[ 1 -( 4 '- methoxybiphenyl-4 - sulfonylamino )- 2 - methylpropyl ] phosphonate . -
pubmed
Glucose regulation of
CDK7 , a putative
thiol related gene , in experimental
diabetic nephropathy . -
pubmed
MAIN OUTCOME MEASURES : Relationships between serum
iron , adiposity , and
serum transferrin receptor ,
C - reactive protein ,
ferritin , and
iron intake analyzed by analysis of covariance and multiple linear regression . -
pubmed
RESULTS : Serum
iron was lower ( 75.8+/-35.2 vs 86.5+/-34.2 g / dl , P=0.002 ) , whereas
transferrin receptor ( 22.6+/-7.1 vs 21.0+/-7.2 nmol / l , P=0.026 ) ,
C - reactive protein ( 0.75+/-0.67 vs 0.34+/-0.67 mg / dl , P<0.0001 ) and
ferritin ( 81.1+/-88.8 vs 57.6+/-88.7 microg / l , P=0.009 ) were higher in obese than non - obese subjects . -
pubmed
Obese subjects had a higher prevalence of
iron deficiency defined by serum
iron ( 24.3 % , confidence intervals ( CI ) 19.3 - 30.2 vs 15.7 % , CI 11.0 - 21.9 % , P=0.03 ) and
transferrin receptor ( 26.9 % , CI 21.6 - 33.0 vs 15.7 % , CI 11.0 - 21.9 % , P=0.0078 ) but not by
ferritin ( 9.8 % , CI 6.6 - 14.4 vs 9.3 % , CI 5.7 - 14.7 % , P=0.99 ) . -
pubmed
Transferrin receptor ,
ferritin and
C - reactive protein contributed independently as predictors of serum
iron . -
pubmed
B19 - infected plasma was used to test whether incubation of
amotosalen with virus before
PCT enhanced inactivation compared to immediate PCT . -
pubmed
CONCLUSION : Under defined conditions , PCT with
amotosalen combined with UVA light can be used to inactivate
B19 , a clinically significant virus that can be transmitted through blood transfusion , and heretofore has been demonstrated to be refractory to inactivation . -
pubmed
In the present study we aimed to assess the role of
MMPs in experimental
cardiomyopathy induced by repeated 10 - week administration of
daunorubicin ( 3 mg / kg i.v . ) -
pubmed
In the
daunorubicin group , the plasma cardiac
troponin T levels (
cTnT - a marker of
myocardial necrosis ) were significantly increased ( p<0.05 ) , commencing with the 8th administration compared with the controls . -
pubmed
The amount of
collagen ( an estimate of
fibrosis ) was also significantly higher in the
daunorubicin group ( 13.39 + /- 0.97 mg / g wet weight ) compared to the control group ( 10.03 + /- 0.65 mg / g wet weight ) . -
pubmed
Evaluation of the proposed method was performed by manual segmentation of chronic and enhanced ( early ) acute lesions in
gadolinium enhanced
T1 - weighted ( Gad - E -
T1 - w ) images by studying
T1 - weighted (
T1 - w ) and
T2 - weighted (
T2 - w ) images , using similarity criteria . -
pubmed
AIM : To determine whether endurance training can counterbalance the negative effects of
hypoxia on mitochondrial phosphorylation and expression of the long chain mitochondrial
fatty acid transporter muscle
carnitine palmitoyl transferase 1 (
mCPT-1 ) . -
pubmed
RESULTS : Chronic
hypoxia decreased basal ( V(0 ) , -31 % with
pamitoylCoA +
carnitine and -21 % with
palmitoylcarnitine , P & lt ; 0.05 ) and maximal ( V(max ) , -31 % with
pamitoylCoA +
carnitine , P & lt ; 0.05 ) respiration rates ,
hydroxyacylCoA dehydrogenase activity ( -48 % , P & lt ; 0.05 ) ,
mCPT-1 activity index ( -34 % , P & lt ; 0.05 ) and
mCPT-1 protein content ( -34 % , P & lt ; 0.05 ) . -
pubmed
BACKGROUND : Based on the outcome of several randomized controlled trials , the orally active
leukotriene receptor antagonist
montelukast (
Singulair , Merck ) has been licensed for treatment of
asthma . -
pubmed
Pharmacological differences of
glitazones : does
peroxisome proliferator - activated receptor - alpha activation make the difference ? -
pubmed
RESULTS : Two - week preoperative treatment with
tiotropium significantly improved respiratory symptoms and
pulmonary function as reflected by FVC ( median 3.43 L pretreatment vs 3.52 L
post - treatment ) , FEV(1 ) ( median 2.06 L vs 2.32 L ) and FEV(1)% ( 73.2 % vs 81.0 % ) ( all P & lt ; 0.001 ) . -
pubmed
Enzyme activities of
acetyl - CoA carboxylase (
ACC ) and
fatty acid synthase (
FAS ) as well as
diacylglycerol acyltransferase (
DGAT ) and
3 - hydroxy-3 - methyl - glutaryl - CoA reductase (
HMG - CoA - R ) , pace - setting steps of de novo
fatty acid ,
triacylglycerol (
TAG ) and
cholesterol synthesis respectively were assayed in
digitonin - permeabilized hepatocytes . -
pubmed
It has been demonstrated that
ORP150 binds
tumor - associated
Ag peptides within
cancer cells . -
pubmed
Most recently , it has been shown that exogenous
ORP150 induces cross - presentation of a chaperoned
Ag , thereby stimulating
Ag - specific CTLs . -
pubmed
Our data indicate that targeting of the
Ag to a " static " early endosme by
ORP150 is required for the efficient cross - presentation . -
pubmed
Nonlinear Hammett plots in pyridinolysis of
2,4 - dinitrophenyl X - substituted benzoates : change in
RDS versus resonance contribution . -
pubmed
The Bronsted - type plots for
pyridinolysis of 1a - j are linear with beta(nuc ) = 0.74 - 0.98 , indicating that the reaction proceeds through a stepwise mechanism in which the second step is the
RDS . -
pubmed
It has been concluded that the electronic nature of the substituent X in the
benzoyl moiety does not influence the
RDS , but the degree of bond formation ( or the effective charge on the nucleophilic site ) in the transition state becomes more significant as the substituent X changes from a strong EDG to a strong EWG . -
pubmed
Development of specific inhibitors of
CYP707A , a key enzyme in the catabolism of
abscisic acid . -
pubmed
Catabolic inactivation of
ABA is mainly controlled by
ABA 8 '- hydroxylase (
CYP707A ) , which is the
cytochrome P450 catalyzing the C8'-hydroxylation of
ABA into
8 '- hydroxy - ABA and its more stable tautomer ,
phaseic acid , which has much lower hormonal activity than
ABA . -
pubmed
Thus , a specific inhibitor of
CYP707A is promising not only as a chemical probe for the mechanism of
ABA action , but also because of its potential use in agriculture and horticulture . -
pubmed
The novel
histone deacetylase inhibitor ,
AR-42 , inhibits
gp130 /
Stat3 pathway and induces apoptosis and cell cycle arrest in multiple
myeloma cells . -
pubmed
Preclinical data indicate
cisplatin sensitivity , suggesting that these
tumors may have defects in the
BRCA1 pathway . -
pubmed
Heme - regulated eukaryotic initiation factor 2alpha kinase (
HRI ) functions under conditions of
heme shortage caused by
blood diseases such as
erythropoietic protoporphyria and
beta - thalassemia , and retains the
heme :
globin ratio at 1:1 by sensing the
heme concentration in reticulocytes . -
pubmed
It was found that [ 1 ] an interaction between
Hsp90 and phosphorylated
HRI was evident , whereas no interaction was observed between
Hsp90 and
HRI dephosphorylated by treatment with
lambda protein phosphatase ; [ 2 ]
Hsp90 enhanced the
kinase activity of phosphorylated
HRI but not dephosphorylated
HRI , but this enhancement was not observed in the presence of
heme ; and , [ 3 ] autophosphorylation of
HRI was not influenced by
Hsp90 . -
pubmed
Therefore , we propose that autophosphorylation of
HRI is critical for catalytic regulation by
Hsp90 under
heme - shortage conditions . -
pubmed
We showed that
Naph exhibited effective preferential cell growth inhibition via
G2 / M phase arrest and apoptosis , which was associated with reduced levels of
Cdc2 and
Cdc25C expression . -
pubmed
Naph also increased
cleaved caspase-3 and
Poly ADR ( adenosine diphosphate ribose ) Polymerase expression ,
gamma - H2AX expression ( an indicator of DNA double strand breaks ) and DNA fragmentation . -
pubmed
The cell lines were transfected or irradiated with US with pGL3 control (
luciferase reporter plasmid ) , and a
luciferase assay ( 48 h later ) or an
MTS assay ( 72 h later ) were performed . -
pubmed
Design and evaluation of a series of
pyrazolopyrimidines as
p70S6 K inhibitors . -
pubmed
High throughput screening versus
p70S6 K led to the identification of
aminopyrimidine 3a as active inhibitor . -
pubmed
We included 576 subjects ( 229 controls , 193
AD , and 154
mild cognitive impairment subjects who converted to
AD ) from the ADNI study , for whom baseline data on cerebrospinal fluid
amyloid - beta (
Abeta)42 ,
phosphorylated tau ( p - tau ) , and total - tau ( t - tau ) , hippocampal volumes , and
FDG - PET were available . -
pubmed
To ensure the quality of the filter system every 7 days metabolic parametres such as WBC count ,
haemoglobin content ,
haemolysis rate ,
potassium load , pH and
ATP content were analysed over a storage period of 49 days . -
pubmed
During whole storage time the
ATP level was maintained above 2.0 mumol per g
haemoglobin . -
pubmed
RESULTS :
Lysozyme was found to be substantially bound onto negatively charged vesicles via electrostatic interaction as evidenced by zeta potential measurements regardless of
cholesterol content . -
pubmed
In contrast , the size of phospholipid vesicle - bound
lysozyme became larger with the increasing
cholesterol content . -
pubmed
Furthermore , serum high - sensitivity
C - reactive protein ,
8 - isoprostane and
superoxide dismutase were similar among the three tertiles of patients ( all P & gt ; 0.05 ) . -
pubmed
Enzymatic assays of
RcLPAT2 indicate a preference for
ricinoleoyl - CoA over other
fatty acid thioesters when
ricinoleoyl - LPA is used as the
acyl acceptor , while
oleoyl - CoA is the preferred substrate when
oleoyl - LPA is employed . -
pubmed
RcLPATB groups with
B - class LPAT enzymes described as seed specific and selective for unusual
fatty acids . -
pubmed
However ,
RcLPATB exhibit a broad specificity on the
acyl - CoAs , with
saturated fatty acids ( 12:0 - 16:0 ) being the preferred substrates . -
pubmed
RcLPATB is upregulated coinciding with seed
triacylglycerol accumulation , but its expression is not restricted to the seed . -
pubmed
abortus 2308 ,
MucR regulates a wide variety of genes whose products may function in establishing and maintaining cell envelope integrity , polysaccharide biosynthesis ,
iron homeostasis , genome plasticity , and transcriptional regulation . -
pubmed
The hypothesis for the enhanced stereoselectivity has been that the
benzylidene locks the molecule in a less reactive conformation with the
O6 trans to the ring
oxygen (
O5 ) , which would stabilize the formed
alpha - triflate and subsequent give beta - selectivity . -
pubmed
In this work , the hypothesis is challenged by using the
carbon analogue (
C7 ) of the
benzylidene - protected
mannosyl donor , which is investigated in terms of diastereoselectivity and reactivity and by low - temperature NMR . -
pubmed
Furthermore , the
mdVp1s formed multiprotein complexes with
LT ,
Hsp70 , and
Hsp40 , and a fraction of the largest
mdVp1 ,
md4 , was
disulfide linked to the unmodified
Vp1DeltaC. -
pubmed
Only cotreatment , but not the single exposure to
limonene and
linalyl acetate , replicated distinctive morphological and biochemical changes induced by BEO , including
caspase-3 activation ,
PARP cleavage , DNA fragmentation , cell shrinkage , cytoskeletal alterations , together with necrotic and apoptotic cell
death . -
pubmed
The data suggest that the
endolymphatic enlargement caused by a loss of
Slc26a4 is a consequence of disrupted
Na (+ ) transport . -
pubmed
The collateral perfusion pressure changes to
AVP were lower in
STZ - injected groups , which were reversed by
OPC-31260 ( a
V2R antagonist ) and overcome by
NaF ( a
G protein activator ) . -
pubmed
3 and 4 are able to react with
9 - ethylguanine (
9 - EtG ) yielding the corresponding monoadduct
[ Pt ( dmba )( L )( 9 - EtG )](+ ) derivatives as followed by ESI -
MS . -
pubmed
Compound 3 interacts mainly with double - stranded (
DS ) oligonucleotides as shown by analysis with ESI -
TOF -
MS , being also able to displace
ethidium bromide ( EB ) from DNA , as observed by an electrophoretic mobility study . -
pubmed
Theoretical calculations at the COSMO(
CHCl3)/B3LYP - D / def2 - TZVPPecp//B3LYP - D / def2 - TZVPecp level and energy evaluations at the COSMO(
CHCl3)/PWPB95 -
D3 / def2 - TZVPPecp level of theory on compound 4 and model systems have been done . -
pubmed
This was mainly due to postnatal increases in
LA - containing PC and
PE , resulting in decreased fractions of their
DHA- and
ARA - containing counterparts . -
pubmed
These changes are also reflected by
PE molecular composition as an indicator of altered hepatic
fatty acid homeostasis . -
pubmed
A surface receptor crosslinking assay was conducted to examine the response of individual
NMDA receptor subunits to
dopamine depletion in isolated and confined surface and intracellular compartments of striatal neurons . -
pubmed
The
NMDA receptor
GluN2B subunit was elevated in its abundance in the surface pool of the lesioned striatum , while intracellular
GluN2B levels were not altered . -
pubmed
CONCLUSION : These results demonstrate the differential sensitivity of principal
NMDA receptor subunits to
dopamine depletion . -
pubmed
GluN1 and
GluN2B expression in the distinct surface compartment underwent upregulation in striatal neurons after selective lesions of the dopaminergic pathway by
6 - OHDA . -
pubmed
MATERIALS AND METHODS : In this experimental study , we have treated hESCs with 200 nM of
Stauprimide (
Spd ) for one day followed by
activin A ( 50 ng / ml ; A50 ) for the next three days ( Spd - A50 ) . -
pubmed
The use of higher concentrations of
Spd ( 400 and 800 nM ) in the
Spd - A50 protocol caused an increase in the expression
SOX17 as well as a dramatic increase in mortality rate of the hESCs . -
pubmed
Therefore , while the
Spd - A50 protocol led to DE production , we have shown that
IDE1 / 2 could not fully replace
activin A in DE induction of hESCs . -
pubmed
Structure - activity relationship (
SAR ) analysis showed that the anti - proliferative activity was affected by A - ring - substituent ( -OCH3>-CH3>-H>-Br>-
Cl>-F ) . -
pubmed
Docking simulation of compound 7d into
EGFR active site showed that
naphthalene ring of 7d with
LYS721 formed two p - pi bonds , which enhanced antitumor activity . -
pubmed
Various
thioglycoside derivatives were obtained in high yields by one -
pot fluoride - mediated de - silylation and
thiol alkylation with
alkyl halides or Michael acceptors of one common
TIPS S - glycoside . -
pubmed
Additional serum and plasma samples were used to measure concentrations of
thromboxane B2 (
TXB2 ) and
prostaglandin E2 (
PGE2 ) , to assess
COX-1 and - 2 inhibition , respectively . -
pubmed
N -[ 3,4 - dimethoxycinnamoyl ]- anthranilic acid (
tranilast ) inhibits
transforming growth factor beta , alleviates
allergic reactions , and decreases
hypertrophic skin scars . -
pubmed
Histological evaluation of
ionized calcium binding adaptor molecule 1 (
Iba1 ) at 1 week after
SCI and
glial fibrillary acidic protein (
GFAP ) ,
fibronectin , and
chondroitin sulfate (
CS ) at week 8 was performed . -
pubmed
At week 8 , although the number of
GFAP - positive cells increased greatly from the impaction site to the proximal and distal sites in the control group , these cells were confined around a cavity in the
tranilast - treated groups . -
pubmed
Anti - CS antibody level in the
tranilast - treated groups was significantly lower than that in the control group . -
pubmed
Levels of
serotonin (
5HT ) ,
norepinephrine ( NE ) and
dopamine ( DA ) were quantified in the CSF after 1 h of sham , standard and microburst VNS with a
wash - out period of 1 month . -
pubmed
One week after the
CSF experiment , the
PTZ seizure threshold was determined after the same stimulation paradigm . -
pubmed
SIM ( selected ion monitoring ) was applied to isolate
fatty acid methyl esters on
TIC ( total ion current ) chromatograms , using the 74 Da fragment ion , which originated from McLafferty rearrangement , and is typical for
methyl - esters . -
pubmed
GC -
MS system was used for the trans - esterification of
triglycerides to
fatty acid methyl esters in the gas chromatographic injector . -
pubmed
STUDY DESIGN : Intact deciduous teeth with different
root resorption stages were cryopreserved using
FBS and
Dimethyl Sulfoxide (
DMSO ) medium ( 9:1 ) in a progressive freezing process , by placing the samples in the refrigerator ( 4 degrees C/60 min ) and subsequently transferring them to a -80 degrees C freezer ( controlled device -1 degrees C / min/24 hours ) , and finally into liquid
nitrogen ( -196 degrees C/30 days ) . -
pubmed
Chromatographic separation of antimalarial drugs and internal standard (
ibuprofen ) was achieved on a
C18 column with a mobile phase composed of 10 mM
dipotassium orthophosphate at pH 3.0 ,
methanol , and
acetonitrile in a ratio of 20:38:42 v / v , at a flow rate of 1 mL / min . -
pubmed
The mechanism of action of
DSF for treating these addictions is unclear but it is unlikely to involve the inhibition of liver
aldehyde dehydrogenase (
ALDH2 ) . -
pubmed
An internal standard , the
N , N - di - isopropylcarbamoyl homolog ( MIM : 291>128 ) is easily separable from
DETC - NAC ( MIM : 263>100 ) on
C18 RP media with a
methanol gradient . -
pubmed
However , these techniques are known to be problematic when applied to highly polymorphic genomic regions , such as the human leukocyte
antigen (
HLA ) genes . -
pubmed
Reactions between
2,6 - diformyl-4 - alkyl ( R )- phenol ( R =
CH3 or
C ( CH3 ) 3 ) and
1,3 - diamino-2 - hydroxypropane (
1,3 - DAP ) in the presence of
copper ( II ) salts (
Cu ( BF4 ) 2 6H2O ,
Cu ( ClO4 ) 2 6H2O /
H3BO3 /
Ar ) and
triethylamine (
TEA ) in a single
pot result in self - assembly of dimeric
dodecacopper supramolecular architectures of 30 - membered
hexatopic macrocyclic ligands (
H6L4 and
H6L5 ) with unique and fascinating structures having the
BO3 ( 3 - ) anion as the central species bonded to all six
copper centers in a symmetrical fashion (
mu6 - BO3 ( 3 - ) ) . -
pubmed
Thereafter , the rewarding effects of
nicotine were evaluated through the conditioned place preference (
CPP ) and self - administration (
SA ) tests . -
pubmed
In stark contrast ,
MEL1 hES cell
amino acid and
carbohydrate use and mitochondrial function were relatively unaltered in response to
oxygen . -
pubmed
Here , we have demonstrated that
Hra1 mediates autoaggregation by self - association and we hypothesize that at least some surface - exposed
amino acid sequences that are present in
Hra1 , but absent in
Tia , represent autoaggregation motifs . -
pubmed
In
Hra1 we swapped nine candidate motifs in three of these loops , ranging from one to ten
amino acids in length , to the corresponding sequences in
Tia . -
pubmed
METHOD : We primarily assessed whether
pitavastatin could help mitigate the development of
emphysema induced by continuous cigarette smoking (
CS ) exposure . -
pubmed
CONCLUSION : Increased
AQP1 and
AQP4 levels in the heart tissue may be correlated with the maintenance of water and
electrolytes balance , which decreases with aging . -
pubmed
TRP channels are classified into seven subfamilies based on the
amino acid sequence homology , and
transient receptor potential melastatin 2(
TRPM2 ) is the second member of the TRPM subfamily . -
pubmed
The single target
lysine of
PanB was identified by a combination of mass spectroscopy and mutational analysis . -
pubmed
Titanium dioxide nanoparticles (
TiO2 NP ) are broadly used in a wide range of applications . -
pubmed
In this study we investigated
cytotoxicity ,
genotoxicity and epigenetic properties of
TiO2 NP uncoated and coated with
silica or
citrate , as well as of the benchmark material P25 . -
pubmed
Our results showed that the uncoated
TiO2 NP were both cytotoxic and genotoxic , and the remediation strategy adopted did not reduce the adverse effects of uncoated
TiO2 NP . -
pubmed
In particular , the presence of
citrate was able to increase their
cytotoxicity and
genotoxicity , exerting also epigenotoxic effects , as evaluated by the marked reduction of
LINE-1 methylation levels . -
pubmed
The structural , optical , and excitonic characteristics of the resultant
MAPbI3 thin films were analyzed using X - ray diffraction ( XRD ) , atomic - force microscopy , absorbance spectroscopy , photoluminescence (
PL ) spectrometry , and nanosecond time - resolved PL . -
pubmed
AIM : To optimize the production of pH - sensitive
dapsone (
DAP ) nanoparticles based on Eugradit L100 ( NPs - EL100 -
DAP ) for oral delivery . -
pubmed
RESULTS : The in vitro release assay of NPs - EL100 -
DAP confirmed the nanoparticles ' pH sensitivity and the ability to deliver
DAP at intestinal environment . -
pubmed
OBJECTIVE :
Tofacitinib is an oral
JAK inhibitor indicated for the treatment of
rheumatoid arthritis (
RA ) . -
pubmed
The dimeric excerpt
{ ( mu - bipy )[ FeL1 ( MeOH )] 2 } 2MeOH of the coordination polymer chain crystallizes in the triclinic space group
P1 and reveals that the packing of the molecules in the crystal is dominated by
hydrogen bonds . -
pubmed
Effects of
glucose -
insulin infusion during major oral and maxillofacial surgery on
postoperative complications and outcomes . -
pubmed
Our aim is to elucidate the effects of intraoperative glycemic control by
glucose -
insulin ( GI ) infusion on
postoperative complications and outcomes in major oral and maxillofacial surgery . -
pubmed
In the GI group , regular
insulin was continuously applied with
glucose - added
acetate Ringer 's solution ( 5 - 10 g
glucose per 500 mL ) . -
pubmed
The mean infusion rate of
glucose during surgery ( mg / kg / h ) was independently associated with the decrease in both serum
total protein and
albumin concentrations from the control to Day1 . -
pubmed
Conclusions : Application of additional
glucose during major oral and maxillofacial surgery preserved
serum albumin concentration . -
pubmed
Simply linking the two aromatic rings of a
diarylamine to afford a
phenoxazine offers profound differences in
HAT reactivity : 1000 - times greater than
diphenylamine and 10 - fold more reactive than
alpha - tocopherol , Nature 's optimized
phenolic RTA . -
pubmed
Thus ,
phenoxazines are an exciting scaffold for
RTA development . -
pubmed
In recent years , we have found that many
organosulfur compounds exhibit very good
RTA activity . -
pubmed
In particular ,
sulfenic acids (
RSOH ) and
hydropersulfides (
RSSH ) are found to be among the best
HAT agents known , particularly to
peroxyl radicals where secondary orbital interactions are found to play a significant role . -
pubmed
These insights suggest that the
RTA activity of
organosulfur compounds may be as important to the inhibition of
hydrocarbon autoxidation , if not more so , than their ionic reactions . -
pubmed
We identified 545 differentially expressed genes (
DEGs ) , 33 of which were in common between the nMnO2 -
Cd , nHAP20 -
Cd , and nHAP40 -
Cd groups . -
pubmed
Blockage of
HGF signaling by
HGF receptor inhibitor ,
PHA-665752 , inhibited proliferation of hESC -
RPE cells . -
pubmed
Doxorubicin (
DOX ) , a model anticancer drug , was first covalently linked onto the partially acetylated
poly ( amidoamine ) dendrimers of generation 5 (
G5 ) prefunctionalized with
folic acid (
FA ) through acid - sensitive
cis - aconityl linkage to form
G5 NHAc - FA - DOX conjugates , which were then entrapped with gold (
Au ) nanoparticles ( NPs ) to create dendrimer - entrapped
Au NPs (
Au DENPs ) . -
pubmed
Importantly , because of the coexistence of targeting ligand
FA and
Au core NPs as a
CT imaging agent , the multifunctional
DOX - loaded
Au DENPs afford specific chemotherapy and
CT imaging of
FA receptor - overexpressing
cancer cells . -
pubmed
The constructed
DOX - conjugated
Au DENPs hold a promising potential to be utilized for simultaneous chemotherapy and
CT imaging of various types of
cancer cells . -
pubmed
Parvalbumin and
lipoprotein lipase could be firstly selected as key proteins in view of their known functions in regulating the buffering of intracellular free
Ca2 + in both cartilage and bone cells and bone mass , respectively . -
pubmed
Apoptosis in germ cells was quantified by the
transferase biotin -
dUTP nick end labeling assay . -
pubmed
Lactate modulates
CD4 + T - cell polarization and induces an immunosuppressive environment , which sustains
prostate carcinoma progression via
TLR8 / miR21 axis . -
pubmed
Here we demonstrate that in a
prostate cancer model
lactate released by glycolytic
cancer - associated fibroblasts ( CAFs ) acts on
CD4 + T cells , shaping T - cell polarization . -
pubmed
In particular , CAFs exposure ( i ) reduces the percentage of the antitumoral Th1 subset , inducing a
lactate - dependent ,
SIRT1 - mediated deacetylation / degradation of
T - bet transcription factor ; ( ii ) increases Treg cells , driving naive T cells polarization , through a
lactate - based
NF - kB activation and
FoxP3 expression . -
pubmed
This hypothesis was confirmed by the fact that
furfural and
malonate , inhibitors of
pyruvate dehydrogenase and
succinate dehydrogenase (
P cycle enzymes ) , respectively , increased resistance of VA - RCAZ to antibiotics , while exposure to
triclosan , to inhibit biosynthesis of
fatty acids , decreased resistance . -
pubmed
Oxygen concentration ( 0 , 0.5 % and 1 % ) of ionized gas was firstly confirmed to be the main factors for
CP treatment , besides treatment time ( 2 , 4 and 6 min ) . -
pubmed
In terms of
anthocyanin and
vitamin C , relatively shorter exposure time to
CP was more suggested . -
pubmed
CONCLUSIONS : The PRISTINE study demonstrated the feasibility of adding
rifampicin to
beta - lactam antibiotics in the treatment of community - acquired
pneumococcal pneumonia , but , despite solid in vitro and experimental animal research evidence , failed to demonstrate a difference in plasma
LTA concentrations and subsequent inflammatory and clinical responses . -
pubmed
In addition ,
LTA plasma concentration could be too low to mount a response via
Toll - like receptor 2 in vitro , but may nonetheless have an effect in vivo . -
pubmed
Our findings show that the relative expression of
SGLT1 and
GLUT2 mRNA were not affected by
fructose - feeding and/or
chrysin . -
pubmed
This is the first report showing that
chrysin is able to interfere with the effects of
fructose at the intestinal level , which may contribute to the
fructose - induced
MS features . -
pubmed
Silicone oil or rubber with
PDMS can be combined with NR - latex and
CR - latex because of
PVA 's emulsion polymerization behavior . -
pubmed
New
pyrimidine - benzoxazole /
benzimidazole hybrids : Synthesis , antioxidant , cytotoxic activity , in vitro
cyclooxygenase and
phospholipase A2 - V inhibition . -
pubmed
Exaggerated activation of the
renin angiotensin aldosterone system ( RAAS ) is a key feature in diseases such as
hypertension ,
diabetes and
chronic kidney disease . -
pubmed
Mitochondrial
oxygen consumption was evaluated after addition of
Ang II alone or after pre - incubation with
candesartan (
AT1 receptor antagonist ) ,
PD-123319 (
AT2 receptor antagonist ) or the two in combination . -
pubmed
Ang II response was reversed to increased
oxygen consumption by
nitric oxide synthase inhibitor
L - NAME . -
pubmed
In addition , it promoted the differentiation and activation of
CD8 + T lymphocytes , differentiation of
CD14 + monocytes , and stimulated the release of
nitric oxide and
cytokines , mainly those linked to a Th1 response . -
pubmed
AIM : This study aimed to evaluate the effect of
lithium disilicate endocrowns compared to prefabricated
zirconia crown used for restoring pulpotomized primary molars , on their Fracture Resistance and to compare the loads to failure these different ceramic restorations with previously reported posterior occlusal forces . -
pubmed
Group (
G1 ) and the
lithium disilicate (
IPS e.max Press ) Endocrown Group (
G2 ) . -
pubmed
RESULTS : Group
zirconia crown (
G1 ) showed significantly higher
fracture strength than Group (
G2 )
lithium disilicate endocrown ( p < 0.05 ) . -
pubmed
Ribociclib (
LEE011 ,
Kisqali ) is a highly selective small molecule inhibitor of
cyclin - dependent kinases 4 and 6 (
CDK4 / 6 ) , which has been approved for the treatment of advanced or metastatic
breast cancer . -
pubmed
Circuits were primed with blood ,
albumin ,
heparin ,
NaHCO3 , and
CaCl2 . -
pubmed
Considering the remarkably improved device performance , the proposed
HTL of
Cs0.32WO3 is promising acting as a favorable building block for high efficiency light - emitting devices . -
pubmed
ABBREVIATIONS : Continuous Wave : CW ; Code Division Multiple Access : CDMA ; Global System for Mobile Communications : GSM ; Peripheral Blood Mononuclear Cell : PBMC ; Radiofrequency : RF ; Radiofrequency radiation : RFR ; Universal Mobile Telecommunications System : UMTS ; Wideband Code Division Multiple Access : WCDMA ; Specific Absorption Rate :
SAR ; National Toxicology Program : NTP ; amplitude - modulated or amplitude - modulation :
AM ; Electromagnetic frequencies : EMF ; confidence interval : CI ; Gigahertz : GHz ; odds ratio : OR ; incidence ratio : IR ; reactive
oxygen species : ROS ; specific absorption rate :
SAR ; International Agency of Research on
Cancer : IARC ; single - strand breaks :
SSB ; double - strand breaks : DSB (
7,12 - Dimethylbenz [ a ] anthracene ):
DMBA ; Hour : h ; international commission on non - ionizing radiation protection : ICNIRP ; extremely low frequency : ELFl ; microtesla : mT ; Gigahertz : GHz ; hertz : Hz ; decibel : dB ; kilometer : Km ; Watt per square meter : W / m2 ; Hour : h ; positron emission tomography : PET . -
pubmed
MSC090213JE08 and its
unsaturated fatty acid moiety has been confirmed in vitro to be synthesized by a
type II PKS . -
pubmed
Biosynthesis of such a highly reduced
polyketide by a
type II PKS is worthy of note . -
pubmed
0.6 ( at pH 7.3 ) under stimulation of both
photosystems with a short
xenon flash of light . -
pubmed
The effect of
ribavirin was evaluated by the clinical picture and by the changes in both direct and total serum
bilirubin and in the activity of serum
glutamic - pyruvic and glutamic - oxaloacetic transaminases . -
pubmed
There was a significant decrease in serum
bilirubin ,
SGOT , and
SGPT from the 5th to 10th day of treatment in the
ribavirin group . -
pubmed
[ Action of increasing doses of
DL - propranolol on behavior of arterial pressure and
plasma renin activity ( PRA ) ] . -
pubmed
It may be responsible for a temporary elevation of the
LH level in the blood triggering final maturation of the ovarian follicles and an increase of
estrogen secretion . -
pubmed
An
LH - inhibiting activity not related to the negative
estrogen feedback is exerted by this nuclear region during critical periods of sexual maturation . -
pubmed
Positive inotropic action of
hemolysin was observed , and the
Na -
Ca exchange mechanism was enhanced after
hemolysin treatment . -
pubmed
The hydrolysis of
AA-2 G on the mucosal surface of everted gut was completely inhibited by an
alpha - glucosidase inhibitor and the hydrolytic activity of a crude membrane extract diminished to one - forth after immunoprecipitation with the
antibody specific to
maltase . -
pubmed
From these results , it is concluded that ingested
AA-2 G serves as a
vitamin C source through the hydrolysis by intestinal membrane - bound
alpha - glucosidase , mainly
maltase , and the subsequent absorption of released
ascorbic acid . -
pubmed
The proper disk mass for diffusion susceptibility tests with
cefoperazone - sulbactam was determined by using a predictor panel of clinical isolates that included staphylococci and gram - negative bacteria intrinsically susceptible , intrinsically resistant , and of various susceptibilities because of the production of different types and amounts of
beta - lactamase . -
pubmed
Collectively , these results demonstrate that accumulation of
plasmalogen catabolites resulting from activation of
plasmalogen - selective
phospholipases A2 can potentiate alterations in membrane dynamics during signal transduction in
plasmalogen - enriched bilayers . -
pubmed
We found that both PD + FET and
PEP + FET improved whole lung
TBC at 30 minutes and 1 h four or fivefold ( p less than 0.01 ) compared with control , whereas at 2 h and 3 h only the improvement following
PEP + FET ( approximately 1.4 times ) was significant ( p less than 0.05 ) . -
pubmed
There was no significant difference in whole lung or regional
TBC between the PD + FET and
PEP + FET treatments . -
pubmed
A unique stimulation of
BBMV ATPase activity occurred at acidic pH in the presence of 1 mM
ZnCl2 . -
pubmed
In the absence of
Zn2 + , a second
ATPase activity was stimulated by alkaline pH values with peak stimulation occurring between pH 8.5 and 9.0 . -
pubmed
The results suggest that the alkaline pH - stimulated hydrolysis of
ATP probably represents the activity of
BBMV alkaline phosphatase . -
pubmed
The unique acidic pH +
Zn2 (+)-stimulated
ATPase activity must represent the activity of a second protein other than the
alkaline phosphatase , since purified
alkaline phosphatase did not show this activity . -
pubmed
The deduced
IME1 product is a 360 - amino - acid protein with a
tyrosine - rich C - terminal region . -
pubmed
PGAL1 -
IME1 expression did not cause growing cells to sporulate but permitted efficient sporulation of
amino acid - limited cells , which otherwise sporulated poorly . -
pubmed
To determine whether Sertoli cells and gonocytes are functionally coupled in the cocultures , we used the glass bead - loading technique of McNeil and Warder to introduce
Lucifer yellow ( LY ) , a gap junction - permeant probe , and
Rhodamine - dextran (
RD ) , a larger marker excluded by gap junctions , simultaneously into cultures 24 h after plating . -
pubmed
We have developed two region - specific immunoassays capable of measuring
PTHRP in plasma : an immunoradiometric assay directed toward
PTHRP amino acid sequence 1 to 74 and a radioimmunoassay directed toward
PTHRP amino acid sequence 109 to 138 . -
pubmed
The mean ( geometric ) urinary
kallikrein activity ( in U / g
creatinine ) amounted to 0.52 ( range 0.11 - 1.90 ) in the control group ( n = 193 ) against 0.39 ( range 0.10 - 1.03 ) in the
cadmium group , and the prevalence of abnormally low activity levels ( less than or equal to 0.20 U / g
creatinine ) amounted to 17.2 % in the
cadmium group against 5.2 % in the control group . -
pubmed
There was no indication that moderate exposure to
mercury vapour ( n = 53 ;
mercury in urine , range 11 - 224 micrograms / g
creatinine ; average duration of exposure : six years ) or to inorganic lead ( n = 23 ; lead in blood , range 40 - 67 micrograms/100 ml ; average duration of exposure : eight years ) was associated with a reduction of
kallikrein production by the kidney . -
pubmed
The nutritional requirements of the microorganism must be satisfied and the deleterious effect of
oxygen metabolites and of
lysosomal hydrolases must be adequately counteracted . -
pubmed
Glycoprotein IIb - IIIa is the most prominent
Arg - Gly - Asp ( RGD)-binding adhesion receptor on platelets . -
pubmed
When an
octyl glucoside extract of surface - radioiodinated platelets was applied to an affinity matrix of KYGRGDS - coupled Sepharose 4B , a 160 - kDa - labeled protein (
P160 ) and
GPIIb - IIIa bound and were specifically eluted by soluble GRGDSP peptide , but not by the variant GRGESP peptide . -
pubmed
Characterization of
P160 by two - dimensional
sodium dodecyl sulfate - polyacrylamide gel electrophoresis and by the O'Farrell gel electrophoresis system indicated that
P160 is a component of
platelet GPIc . -
pubmed
This possibility was further substantiated by the complete identity between the determined
amino - terminal sequence of
P160 and the known sequence of the
VnR alpha subunit . -
pubmed
The
beta 2 - selective antagonist
ICI 115881 reversed the effects of
epinephrine , whereas the
beta 1 - antagonists
practolol and
betaxolol had little effect . -
pubmed
Forskolin ,
cAMP analogues , and the
phosphodiesterase inhibitor
3 - isobutyl-1 - methylxanthine also effectively inhibited ConA - stimulated
histamine release . -
pubmed
In order to assess the induction of
SOD while protecting against the inflammatory process , topical
dexamethasone (
dex ) was administered t.i.d . -
pubmed
Dex prevented the conjunctival
hyperemia , vascular injection and
iritis seen with endotoxin treatment alone and blocked the induction of
SOD . -
pubmed
A similar
medrysone ( med ) application ( n = 4 ) failed to prevent the visible signs of
ocular inflammation yet also blocked the elevation of
SOD . -
pubmed
Pretreatment with either of the
cyclooxygenase inhibitors ,
indomethacin ( n = 4 ) or
aspirin ( n = 7 ) , failed to block the induction in
SOD ( p less than 0.001 and p less than 0.01 , respectively ) . -
pubmed
However , the induction of
SOD was prevented by the
phospholipase A2 inhibitor ,
quinacrine , and the
lipoxygenase inhibitor ,
nordihydroguaiaretic acid (
NDGA ) . -
pubmed
Conformational changes of the
Ca2 + - ATPase as early events of
Ca2 + release from sarcoplasmic reticulum . -
pubmed
The levels of the phosphorylated intermediate (
EP ) and the
tryptophan fluorescence of the
Ca2 + - ATPase were monitored during both the
Ca2 + accumulation and the induced
Ca2 + release using fast kinetic techniques . -
pubmed
During
Ca2 + uptake , both the
EP level and the
tryptophan fluorescence gradually decreased following a time course similar to that of the
Ca2 + accumulation . -
pubmed
Upon inducing
Ca2 + release by addition of either
caffeine or
ADP , there was a transient increase of the
EP level ( from 0.3 - 0.5 to 1 - 2 nmol / mg protein ) preceding the release of
Ca2 + . -
pubmed
These results indicate that the
Ca2 + - ATPase enzyme undergoes a rapid conformational change in response to triggering of
Ca2 + release . -
pubmed
In glial cultures treated with 0.25 mM
dibutyryl cyclic AMP in the same culture conditions ,
PST activity is suppressed to about 25 % of its untreated counterpart , even though
dibutyryl cyclic AMP at concentrations of ImM only slightly inhibited
PST activity in vitro . -
pubmed
The effects of
Mg2 + ions or
EDTA on nuclear integrity and apparent subcellular distribution of unoccupied
oestrogen receptors in
breast cancer cells . -
pubmed
Homogenisation and fractionation of cells in the presence of
Mg2 + or
EDTA resulted in unoccupied
oestrogen receptor being recovered in the particulate fraction . -
pubmed
In
Mg2 + -prepared fractions , both
5 '- nucleotidase and unoccupied receptor were distributed between plasma membrane , partially - pure nuclei and mitochondrial / microsomal pellets . -
pubmed
Mg2 + -prepared nuclei , freed of membranous contamination by pelleting through 1.8 M
sucrose , were intact by electron microscopy but had no
5 '- nucleotidase or unoccupied receptor . -
pubmed
[
Tyrosinase - oxidoreductase ;
monophenol ,
o - diphenol :
O2 ] . -
pubmed
USA , in press ):
m - xylene , a substrate of the degradative pathway , binds to the
xylR gene product ; the
m - xylene -
xylR product complex activates the
xylS gene ; and the
xylS product thus synthesized de novo activates the
xylDLEGF operon . -
pubmed
The authors report that the reversible decrease in
calcium ,
sodium and
potassium , resulting from an increase in cerebral
lithium levels , can be demonstrated on sampling at 1.30 and 3 hours (
IP ) , or at 12 hours ( VO ) . -
pubmed
Phosphorylation of
ribosomal protein S6 plateaued at around 2 mol of
phosphate / mol of protein with both substrates . -
pubmed
Peptide map analyses showed that the most prominent phosphorylation sites associated with 40 S substrates were the adjacent
serines in the
Arg - Leu - Ser - Ser - Leu - Arg segment of
S6 . -
pubmed
A larger fragment of
S6 ( termed Tlc ) isolated from mild tryptic digests of whole ribosomes , consisted of the T1a sequence extended by the sequence
Ser - Glu - Glu - Ser - Gln-(
Lys ) at the
COOH terminus . -
pubmed
Alpha 2 - adrenoceptor - mediated inhibition of electrically evoked [ 3H]
noradrenaline release from chick sympathetic neurons : role of
cyclic AMP . -
pubmed
Both effects of
forskolin were potentiated by the
phosphodiesterase inhibitor
3 - isobutyl-1 - methylxanthine . -
pubmed
The
forskolin - induced increase in overflow was abolished by the Rp - diastereomer of
cyclic AMP - thioate , an antagonist at
cyclic AMP - dependent protein kinases , and
1,9 - dideoxy - forskolin , an inactive analogue at
adenylyl cyclase , had no effect on the evoked overflow . -
pubmed
A 24 - h pretreatment with either
cholera toxin or
forskolin reduced the subsequent
forskolin - induced accumulation of
cyclic AMP and inhibited the stimulation - evoked release . -
pubmed
Basal
cyclic AMP production , however , remained unaltered after
forskolin treatment and was enhanced after 24 h of
cholera toxin exposure . -
pubmed
Expression levels of the
CKS -
troponin I fusion were 8 % of total cellular protein 4 h after induction with
IPTG . -
pubmed
The fusion was affinity - purified over a
TnC affinity column using a
urea - solubilized extract of a crude cell lysate . -
pubmed
TGF - alpha expression was observed in the cells with increased steroidogenesis , including compact
tumor cells and
zona fasciculata cells with lipid depletion , but did not necessarily correlate with production sites of any specific
steroid hormone . -
pubmed
Aortic smooth muscle contains
guanylate - cyclase - coupled 130 - kDa
atrial natriuretic factor receptor as predominant receptor form . -
pubmed
The basal
guanylate cyclase activity in the aortic membranes was 1.0 pmol
cGMP produced.min-1.mg protein-1 at 37 degrees C using
Mn ( 2 +)- GTP as substrate . -
pubmed
TRH mRNA showed a similar pattern to
somatostatin , with a 2.5 - fold increase after 15 min at 4 degrees C. In contrast , hypothalamic
TRH content was significantly decreased after 15 min cold exposure , returning to control values at 30 min . -
pubmed
Long - term potentiation ( LTP ) of synaptic efficacy in MI horizontal pathways could be produced at short ( 0.5 mm ) and long ( 1.0 mm ) distances by theta burst stimulation ( TBS ) , but only during local , transient application of the
gamma - aminobutyric acid - A ( GABAA ) receptor antagonist ,
bicuculline methiodide (
bic ) immediately before TBS . -
pubmed
In animals pretreated with
guanethidine , intravenous administration of the
vasoactive intestinal polypeptide ( VIP ) receptor antagonist ,
[ p - Cl - D - Phe6,Leu17 ]- VIP ( 50 micrograms kg-1 ) significantly attenuated the increase in carotid blood flow and decrease in carotid vascular resistance evoked by trigeminal
ganglion stimulation . -
pubmed
Responses evoked by trigeminal
ganglion stimulation were , however , unaffected by intravenous injection of the
tachykinin NK1 receptor antagonists ,
GR82334 ( 0.3 mg kg-1 ) and
CP-99,994 ( 0.4 mg kg-1 ) ,
calcitonin gene - related peptide ( CGRP ) receptor antagonist ,
CGRP8 - 37 ( 0.9 mg kg-1 ) and the
ganglion blocking agent ,
hexamethonium ( 10 mg kg-1 ) . -
pubmed
Development and characterization of
hydrogen peroxide - resistant Chinese hamster ovary ( CHO ) cell variants --
II . -
pubmed
Further experiments have demonstrated that , under conditions of reduced
NPSH content ( obtained by growing the cells in the presence of a
glutamylcysteine synthetase inhibitor ) , the cytotoxic action of
hydrogen peroxide was very slightly , if at all , augmented in both wild type and resistant cells . -
pubmed
Remarkably , a previously unreported deficient variant ,
G6PD Orissa ( 44 Ala-->Gly ) , is responsible for most of the
G6PD deficiency in tribal Indian populations but is not found in urban populations , where most of the
G6PD deficiency is due to the
G6PD Mediterranean ( 188
Ser-->
Phe ) variant . -
pubmed
We have shown that a
PI3 K product ,
phosphatidylinositol ( PI ) 3,4,5 - trisphosphate , promotes
pleckstrin phosphorylation in platelets . -
pubmed
Since
pleckstrin homology domains are thought to interact with
Gbetagamma heterodimers and/or
PI ( 4,5 ) P2 , we have examined the effects of recombinant
pleckstrins on platelet
PI3Kgamma and
p85 /
PI3 K activities . -
pubmed
Pleckstrin - mediated inhibition of
PI3Kgamma is overcome by excess
Gbetagamma and is restricted to
PI ( 4,5 ) P2 as substrate , i.e. -
pubmed
Consistent with this , activation of
protein kinase C by exposure of platelets to
beta - phorbol diester ( to increase endogenous
pleckstrin phosphorylation ) prior to platelet lysis causes inhibition of
Gbetagamma - stimulatable
PI3 K activity only with respect to
PI ( 4,5 ) P2 substrate . -
pubmed
MK-801 increased
CGRP in a dose - response manner in frontal cortex , and NKA - LI in occipital cortex . -
pubmed
Although the higher
MK-801 dose reduced
seizure duration by 50 % , the ECS induced
NPY - LI increase in striatum , occipital cortex and hippocampus , and
NKA - LI in occipital cortex was not diminished . -
pubmed
Consistent with these findings ,
thalidomide ( which antagonizes
TNF action ) attenuates c - src induction by LPS . -
pubmed
This concept was supported through histologic analysis of
hematoxylin- and eosin - stained sections , as well as by the pattern of immunohistochemical reactivity with antibodies directed against low and high molecular weight
cytokeratins ,
cell adhesion molecules (
CAM 5.2 ) , and
carcinoembryonic antigens . -
pubmed
The percentage of
hearing loss calculated for both ears , using
CPT index , demonstrated the highest values in the group of subjects drinking non - consumable
alcohols and addicted to volatile organic substances . -
pubmed
Progesterone and
transforming growth factor - beta coordinately regulate suppression of endometrial
matrix metalloproteinases in a model of experimental
endometriosis . -
pubmed
Our findings indicate that blocking the action of
TGF - beta opposes
progesterone - mediated suppression of
MMPs and blocks the ability of this
steroid to prevent experimental
endometriosis . -
pubmed
However , we also show that the action of
TGF - beta does not lead to a sustained suppression of
MMPs as observed following
progesterone treatment . -
pubmed
Taken together , our data suggest that in the absence of a normal
progesterone response , common in
ectopic lesions of
endometriosis , sensitivity to
TGF - beta may be altered , resulting in a failure to regulate
MMPs . -
pubmed
A7 ,
A8 ,
A18 and
A19 , respectively , were cured and found to encode at least part of the metabolic pathway involved in the growth on
ortho - chlorinated benzoates . -
pubmed
These data indicate that
TNFR1 and
CD95 are dispensable for deletion of
antigen - specific T cells after
viral infection . -
pubmed
The
DPD activity of
pancreatic tumors may be a useful clinical marker of the responsibility of
fluorinated pyrimidine dosing in
pancreatic cancer . -
pubmed
ATP - stimulated release of
interleukin ( IL )- 1beta and
IL-18 requires priming by lipopolysaccharide and is independent of
caspase-1 cleavage . -
pubmed
Monocytes rapidly processed and released both
IL-1beta and
IL-18 after exogenous
ATP . -
pubmed
Despite its constitutive cytosolic presence ,
IL-18 required lipopolysaccharide priming for the
ATP - induced release . -
pubmed
Release of both
cytokines was blocked completely by a
P2X7 receptor antagonist , oxidized
ATP , and partially by an antibody to
P2X ( 7 ) receptor . -
pubmed
In evaluating the signaling components involved in the
ATP effect , we identified that the
protein - tyrosine kinase inhibitor ,
AG126 , produced a profound inhibition of both
ICE activation as well as release of
IL-1beta /
IL-18 . -
pubmed
Taken together , these results suggest that , although synthesis of
IL-1beta and
IL-18 differ ,
ATP - mediated release of both
cytokines requires a priming step but not proteolytically functional
caspase-1 . -
pubmed
Interaction of the herbicide
glyphosate with its target enzyme
5 - enolpyruvylshikimate 3 - phosphate synthase in atomic detail . -
pubmed
We have identified the interaction of
EPSP synthase with one of its two substrates (
shikimate 3 - phosphate ) and with the widely used herbicide
glyphosate by x - ray crystallography . -
pubmed
Glyphosate appears to occupy the binding site of the second substrate of
EPSP synthase (
phosphoenol pyruvate ) , mimicking an intermediate state of the ternary enzyme.substrates complex . -
pubmed
The elucidation of the active site of
EPSP synthase and especially of the binding pattern of
glyphosate provides a valuable roadmap for engineering new herbicides and herbicide - resistant crops , as well as new antibiotic and antiparasitic drugs . -
pubmed
The
discs large (
Dlg ) protein , or
synapse - associated protein 97 (
SAP97 ) , is a member of the membrane - associated
guanylate kinase family of multidomain scaffolding proteins which recruits transmembrane and signaling
molecules to localized plasma membrane sites . -
pubmed
BACKGROUND : The occurrence of phase III of migrating motor complexes in neonates is inversely related to gestational age , and it can be triggered in some infants by the
motilin receptor agonist
erythromycin . -
pubmed
Immunoreactivity of
SP is enhanced after acute chemical stimulation of
somata . -
pubmed
This enhancement was significantly attenuated by
tamsulosin in the
L6 and
S1 segments . -
pubmed
Tamsulosin can attenuate nociception - induced
SP upregulation within the spinal cord . -
pubmed
The black women , however , had lower plasma
triglyceride levels ( 0.91 + -0.46 vs. 1.22 + -0.60 mmol / L , p<0.0001 ) and a trend toward higher
high - density lipoprotein cholesterol levels ( 1.37 + -0.34 vs. 1.29 + -0.31 mmol / L , p=0.064 ) than the white women . -
pubmed
Plasma total and
low - density lipoprotein cholesterol levels were similar . -
pubmed
In summary , compared to white women , black women had a higher mean
body mass index ,
higher blood pressures , higher
lipoprotein ( a ) and plasma total
homocysteine levels , and greater consumption of saturated fat and
cholesterol . -
pubmed
In order to facilitate further studies on the molecular function of
OMP , its crystal structure has been determined at 2.3 A resolution using multiwavelength anomalous diffraction experiments on
selenium - labeled protein . -
pubmed
Concentrations of 50 and 100 microM of this compound increased
laccase activity when compared with control samples grown without
tannic acid . -
pubmed
The role of
tannic acid was shown to be an inductor of
laccase activity in this fungus , due to the enhancement of expression of the
laccase gene at the transcriptional level . -
pubmed
At 3 months , she developed
hypocalcemia and
hyperphosphatemia with normal levels of
vitamin D and
alkaline phosphatase , and
parathyroid hormone level was low . -
pubmed
DECREASED
GLUTATHIONE IN AGING
RED CELLS . -
pubmed
The role of
Ca ( 2 + ) ions in
tubular toxicity and the effect of
calcium channel blockers on
CsA toxicity were evaluated by measuring intracellular
calcium using the fluorescent dye
Fura-2 AM . -
pubmed
After 90 min incubation ,
LDH was 22.5 % in control PT and 41.9 % in PT treated with 100 micro g / ml
CsA ( P & lt ; 0.001 , n = 11 ) . -
pubmed
Both treatments significantly lowered
triglycerides and increased
high - density lipoprotein (
HDL )
cholesterol levels from baseline ; however , these changes were not significantly different between treatments . -
pubmed
Low - density lipoprotein (
LDL )
cholesterol concentrations increased significantly in subjects who consumed
DHA - enriched eggs ( p = 0.047 vs. control ) . -
pubmed
However , there was no significant increase in
cholesterol carried by small , dense
LDL particles , as determined by nuclear magnetic resonance analysis . -
pubmed
Results of exploratory analyses suggest favorable effects of the
DHA - enriched eggs over ordinary eggs on
triglyceride and
HDL cholesterol levels in subjects with
body mass index & gt ; or = 30 kg / m2 ; the
DHA treatment produced a larger reduction in serum
triglyceride concentration vs. ordinary eggs ( -12.3 vs. 2.1 % ; p = 0.027 ) , and there was a greater increase for
HDL cholesterol in the
DHA - enriched vs. ordinary egg group ( 5.0 vs. 1.1 % ; p = 0.040 ) . -
pubmed
1.0 % ) , and
beta - bourbonene ( 0.6 - 1.5 % ) , in the HD , SDE , MWHD , and
SFE volatile fractions . -
pubmed
Minute ventilation ( VE ) , respiratory rate ( RR ) , endtidal
CO ( 2 ) pressure ( P(ECO(2 ) ) , and arterial
hemoglobin oxygen saturation ( SpO2 ) were measured with a facemask tightly fitted before and during 7 %
CO ( 2 ) inhalation . -
pubmed
This study examined the effect of vegetarianism on skeletal muscle total
creatine (
TCr ) content and
creatine transporter (
CreaT ) gene expression , prior to and during 5 d of
Cr supplementation ( CrS ) . -
pubmed
OBJECTIVES : Previously , we reported that
etoposide oleate associated to a
cholesterol - rich microemulsion ( LDE ) is taken up by malignant cells overexpressing
low - density lipoprotein ( LDL ) receptors . -
pubmed
There was no difference in
silica exposure between patients with cytoplasmic
ANCA (
c - ANCA ) , perinuclear ANCA ( p - ANCA ) , or atypical ANCA . -
pubmed
Skeletal muscle expresses two catalytic subunits , alpha1 and alpha2 , of the
5 '- AMP - activated protein kinase (
AMPK ) , which has been implicated in contraction - stimulated
glucose transport and
fatty acid oxidation . -
pubmed
AMPK activation was associated with increased phosphorylation of
Thr(172 ) of the alpha - subunit , the primary activation site . -
pubmed
Muscle stimulation increased the phosphorylation of
acetyl - CoA carboxylase (
ACC ) , a downstream target of
AMPK , and the rate of
3 - O - methyl - d - glucose transport . -
pubmed
In contrast , increasing the frequency ( & gt;or=5 Hz ) or duration ( & gt;or=5 min ) of contraction activated
AMPKalpha1 and
AMPKalpha2 and increased
AMP concentration and the
AMP /
ATP ratio . -
pubmed
These results suggest that 1 )
AMPKalpha1 is the predominant isoform activated by
AMP - independent phosphorylation in low - intensity contracting muscle , 2 )
AMPKalpha2 is activated by an
AMP - dependent mechanism in high - intensity contracting muscle , and 3 ) activation of each isoform enhances
glucose transport and
ACC phosphorylation in skeletal muscle . -
pubmed
NO-1886 also suppressed fat accumulation in visceral ( 46.9 + /- 10.4 vs 73.7 + /- 14.5 g , P & lt ; .01 ) and subcutaneous ( 43.1 + /- 18.1 vs 68.9 + /- 18.8 g , P & lt ; .05 ) tissues and increased the levels of plasma total
cholesterol and
high - density lipoprotein cholesterol in comparison to the high - fat control group . -
pubmed
In contrast ,
NO-1886 decreased the levels of plasma
triglycerides , nonesterified free
fatty acid ,
glucose , and
insulin . -
pubmed
NO-1886 increased
LPL activity in soleus muscle ( 0.082 + /- 0.013 vs 0.061 + /- 0.016 mumol of free
fatty acid per minute per gram of tissue , P & lt ; .05 ) . -
pubmed
NO-1886 increased the expression of
UCP3 mRNA in soleus muscle 3.14 - fold ( P & lt ; .01 ) compared with the high - fat control group without affecting the levels of
UCP3 in mesenteric adipose tissue and BAT . -
pubmed
In addition ,
NO-1886 did not affect the expression of
UCP1 and
UCP2 in BAT , mesenteric adipose tissue , and soleus muscle . -
pubmed
In conclusion ,
NO-1886 increased the expression of
UCP3 mRNA and
LPL activity only in skeletal muscle . -
pubmed
High resolution electronic spectra of
anisole and
anisole - water in the gas phase :
hydrogen bond switching in the
S1 state . -
pubmed
Our results indicate that a decrease in
P700 activity ( with a resultant increase in P700 unit size ) may be partially responsible for the photoinhibition of algal photosynthetic
carbon dioxide fixation . -
pubmed
These
isozymes were partially purified on a
DEAE -
Trisacryl column . -
pubmed
Comparison of C40/82A and P27A C40/82A
barstar mutants using
19F NMR . -
pubmed
Barstar , an inhibitor of the enzyme
barnase , contains two
phenylalanine residues , three
tryptophan residues , and two
proline residues . -
pubmed
Barstar is cold denatured in the presence of
urea . -
pubmed
BACKGROUND : The
ABCC5 gene encodes an organic anion pump of the
ATP - binding cassette ( ABC ) transporter family , subclass C. The exact physiological function of
ABCC5 however is not known . -
pubmed
Ca ( 2 + ) binding increases the alpha - helix content and causes significant thermal stabilization with a melting temperature increase of approximately 10 degrees C. Functional
Ca ( 2 +)-dependent phospholipid binding sites of
annexin B1 were investigated using mutant proteins . -
pubmed
By changing a conserved acidic
amino acid residue that putatively combines
Ca ( 2 + ) in each domain of
annexin B1 singly or in combination , we found that
Ca ( 2 + ) binding in the first domain is more important than that at the other
Ca ( 2 + ) binding sites . -
pubmed
The effectiveness and safety of topical
PhotoActif phosphatidylcholine - based anti -
cellulite gel and LED ( red and near - infrared ) light on Grade
II - III thigh
cellulite : a randomized , double - blinded study . -
pubmed
CONCLUSIONS : The results of this small but well - documented , randomized , double - blinded study affirms that eight of nine thighs with Grade
II - III
cellulite responded positively to a novel , combined 3 - month treatment program of a
phosphatidylcholine - based , anti -
cellulite gel and LED exposure , as determined by the clinical determinants obtained . -
pubmed
Severe overresponders and underresponders to upshock were further scrutinized for their
calcium content with
( 45 ) Ca and their
Yvc1 electrophysiological activities under patch -
clamp . -
pubmed
The severe underresponders have lower
calcium content but no change in
Yvc1 activity . -
pubmed
Erythritol (
INS 968 ) is an important four -
carbon sugar alcohol in the food industry . -
pubmed
Currently , HPLC and
GC methods are in use for the quantification of
erythritol in natural / processed foods . -
pubmed
We have utilized affinity - purified
erythritol - specific
antibodies generated earlier [ 9 ] to develop an indirect competitive ELISA . -
pubmed
With
erythritol -
BSA conjugate ( 54 mol / mol ; 100 ng / well ) as the coating antigen , a calibration curve was prepared using known amounts of standard
meso - erythritol ( 0.1 - 100,000 ng ) in the immunoassay . -
pubmed
In contrast , all
erythromycin - resistant isolates ( 73 ) were either constitutively resistant to
telithromycin ( 13 isolates with constitutive
erm genes ) or demonstrated
telithromycin D - shaped zone ( 60 isolates with inducible
msr(A ) and/or erm ) . -
pubmed
All these isolates were resistant to
streptogramin A and harbored
vga ( A ) gene ( 1 isolate ) or
vga ( A ) LC gene ( 19 isolates ) . -
pubmed
Thiazide diuretics remain primary first - step agents , especially for uncomplicated
hypertension ;
calcium channel blockers are also efficacious . -
pubmed
The supernatant is removed and 2 ml of Neural Basal Media ( NBM ) containing 2 %
B27 ( vol / vol ) and 0.25 %
Glutamax ( vol / vol ) is added to the cortex which is then re - suspended by pipetting up and down . -
pubmed
The supernatant is then removed and the cortex pellet re - suspended with 2 ml of NBM containing
B27 and
Glutamax . -
pubmed
Furthermore , a single point mutation in the
CD28 cytosolic tail (
tyrosine 188 ) interferes with the ability of
CD28 to preferentially accumulate at the cSMAC . -
pubmed
PKCtheta distribution at the immunological synapse mirrors the distribution of
tyrosine 188 - mutated
CD28 , indicating that
CD28 drives the localization of
PKCtheta even when
CD28 is not localized to the cSMAC . -
pubmed
Mutation of
tyrosine 188 also results in diminished activation of
NF - kappaB , suggesting that
CD28 - mediated localization of
PKCtheta to the cSMAC is important for efficient signal transduction . -
pubmed
The binding of the antigen -
antibody complex was visualized by the addition of commercial
rabbit antisheep IgG antibody labeled with
alkaline phosphatase with subsequent addition of
p - nitrophenyl phosphate substrate to produce a colored product for quantification . -
pubmed
We also briefly illustrate three established methods to assess
HPA axis activity , reactivity , and feedback functioning , namely the
cortisol awakening rise (
CAR ) , Trier Social Stress Test ( TSST ) , and low - dose
Dexamethasone Suppression Test (
DST ) . -
pubmed
This intron - less gene is 1,149 - base pair long encoding 382
amino acids - putative membrane - bound
Delta12 desaturase protein . -
pubmed
The signature
amino acid sequence ' YNNKL ' was also found to be present at the N terminus of the protein , which is necessary and sufficient for
ER localization of enzyme . -
pubmed
However , exogenously supplied
linoleic acid was feebly converted to
linolenic acid suggesting that
LuFAD2 - 2 encodes a functional
FAD2 enzyme and has substrate specificity similar to
LuFAD2 . -
pubmed
Plasma
ADPN levels were directly correlated with age ,
HDL - C and
creatinine , and inversely with
BMI ,
triglycerides and
glomerular filtration rate (
GFR ) . -
pubmed
Multiple regression analysis identified plasma
creatinine and
HDL as the independent factors associated with
ADPN plasma levels . -
pubmed
There were statistically significant reductions in plasma total
cholesterol ,
low - density lipoprotein cholesterol and
triglyceride concentrations after dietary intervention . -
pubmed
Multiple linear regression analyses showed that the AC and CC
CYP7A1 genotypes accounted for 5.2 and 6.2 % of
triglyceride concentration during follow - up and adjusted percent of change of
triglyceride concentration , respectively . -
pubmed
The present study provides evidence that -278A>C polymorphism in the
CYP7A1 gene can modify
triglyceride concentrations in response to a reduced fat diet in a
dyslipidemic male population . -
pubmed
Donepezil , an
acetylcholinesterase inhibitor , is an approved drug for the treatment of
Alzheimer ' s disease (
AD ) . -
pubmed
Multiple linear regression and artificial neural networks ( ANNs ) as feature mapping techniques were used for the prediction of the biomagnification factor (
BMF ) of some
organochlorine pollutants . -
pubmed
Thus , more than 80 % patients with prior failure to nonnucleoside
reverse transcriptase inhibitors could potentially benefit from
etravirine rescue therapy . -
pubmed
No difference in
insulin sensitivity , measured as the
glucose infusion rate necessary to maintain euglycaemia , was observed . -
pubmed
Self - assembly on
phosphoinositide - containing membranes stimulates
dynamin GTPase activity , which is crucial for its function . -
pubmed
Although the
pleckstrin - homology ( PH ) domain is known to mediate
phosphoinositide binding by
dynamin , it remains unclear how this promotes activation . -
pubmed
Here , we describe studies of
dynamin PH domain mutations found in
centronuclear myopathy (
CNM ) that increase
dynamin 's
GTPase activity without altering
phosphoinositide binding . -
pubmed
CNM mutations in the PH domain C - terminal alpha - helix appear to cause conformational changes in
dynamin that alter control of the
GTP hydrolysis cycle . -
pubmed
Potent antifibrotic activity of
mTOR inhibitors
sirolimus and
everolimus but not of
cyclosporine A and
tacrolimus in experimental
liver fibrosis . -
pubmed
CONCLUSIONS :
mTOR inhibition by
sirolimus and
everolimus in experimental
liver fibrosis associates with significantly less
fibrosis progression and portal
hypertension than treatment with
calcineurin inhibitors
tacrolimus and
cyclosporine A. -
pubmed
The day
trip to the forest park significantly reduced blood pressure and urinary
noradrenaline and
dopamine levels and significantly increased serum
adiponectin and
dehydroepiandrosterone sulfate (
DHEA - S ) levels . -
pubmed
Walking exercise also reduced the levels of serum
N - terminal pro - B - type natriuretic peptide (
NT - proBNP ) and urinary
dopamine . -
pubmed
Taken together , habitual walking in forest environments may lower blood pressure by reducing sympathetic nerve activity and have beneficial effects on blood
adiponectin and
DHEA - S levels , and habitual walking exercise may have beneficial effects on blood
NT - proBNP levels . -
pubmed
Pre- and
post - tests of server responses to pregnant - appearing " pseudo - patron " actors ordering
alcohol in experimental ( n = 148 ) and comparison ( n = 183 ) establishments were a key method of evaluating the efficacy of this intervention . -
pubmed
It occurs through two different mechanisms :
Ca ( 2 + ) release from internal stores , involving
phospholipase C ( PLC)-dependent generation of
inositol-1,4,5 - trisphosphate (
IP3 ) and activation of
IP3 sensitive channels in the store membrane , and
Ca ( 2 + ) influx across the plasma membrane . -
pubmed
In the last years , however , the
Ca ( 2 + ) sensor
stromal interaction molecule 1 (
STIM1 ) and the channel protein
Orai1 emerged as the key players in platelet SOCE . -
pubmed
Low - molecular mass proteins rich in
cysteines called metallothioneins (
MT ) can be considered as markers for the pollution of the environment by metals . -
pubmed
Light triggered detection of
aminophenyl phosphate with a quantum dot based
enzyme electrode . -
pubmed
pAPP as substrate is converted by the enzyme
alkaline phosphatase (
ALP ) to generate
4AP as a product . -
pubmed
We hypothesized that
doxycycline , a broad
MMPs inhibitor , was as effective as
simvastatin in reducing the incidence of
plaque disruption . -
pubmed
The results showed that oral administration of
doxycycline resulted in a significant increase in the thickness of the fibrous cap of the aortic plaque whereas there was a substantial reduction of
MMPs expression , local and systemic
inflammation , and
aortic plaque vulnerability . -
pubmed
Both the number and localization of
granzyme B - positive cells were altered by
vitamin A. -
pubmed
Other
cytokines were reduced and
chemokines increased as the disease progressed , but no additional effects of
vitamin A were seen . -
pubmed
Fragments added to the de novo derivatives 3a , 3b , 3c for
GABA binding site , and 1a , 2a , and 3d for BZ binding site contributed to new binding sites and structural stability , further optimizing binding to
GABA or
BZ binding sites . -
pubmed
Derivative structures can be used as starting points for developing high affinity
GABAA receptor agonists with specificity towards
GABA binding site and BZ binding site . -
pubmed
Given the impact of amphipathic small molecules on bilayer properties such as elasticity and intrinsic curvature , we investigated the effects of six commonly used PAs --
cyclooctatetraene (
COT ) ,
para - nitrobenzyl alcohol (
NBA ) ,
Trolox ( TX ) ,
1,4 - diazabicyclo [ 2.2.2 ] octane (
DABCO ) ,
para - nitrobenzoic acid (
pNBA ) , and
n - propyl gallate (
nPG)--on bilayer properties using a
gramicidin A ( gA)-based fluorescence quench assay to probe for
PA - induced changes in the
gramicidin monomer dimer equilibrium . -
pubmed
,
PA adsorption at the bilayer / solution interface that alter the equilibrium constant for gA channel formation , and thus the number of conducting gA channels in the large unilamellar vesicle membrane , will be detectable as changes in the rate of
Tl + influx - the fluorescence quench rate . -
pubmed
Ambient ultrafine particles reduce endothelial
nitric oxide production via S - glutathionylation of
eNOS . -
pubmed
Here , we tested whether ambient ultrafine particles ( UFP , diameter & lt;200 nm ) modulate
eNOS activity in terms of
nitric oxide (
NO ) production via protein S - glutathionylation . -
pubmed
UFP - mediated reduction in
NO production was restored in the presence of
JNK inhibitor (
SP600125 ) ,
NADPH oxidase inhibitor (
Apocynin ) , anti - oxidant (
N - acetyl cysteine ) , and
superoxide dismutase mimetics (
Tempol and
MnTMPyP ) . -
pubmed
UFP exposure increased the
GSSG /
GSH ratio and
eNOS S - glutathionylation , whereas over - expression of
Glutaredoxin-1 ( to inhibit S - glutathionylation ) restored UFP - mediated reduction in
NO production by nearly 80 % . -
pubmed
Thus , our findings suggest that
eNOS S - glutathionylation is a potential mechanism underlying ambient UFP - induced reduction of
NO production . -
pubmed
Carbon dioxide as a
C1 building block for the formation of
carboxylic acids by formal catalytic hydrocarboxylation . -
pubmed
The concentrations of
FX and
NIV in the plasma and various tissues were measured using LC -
MS /
MS . -
pubmed
FX and
NIV were detectable in the vital organs up to 24h after po administration . -
pubmed
In vitro of liver postmitochondrial fractions with
FX demonstrated that the liver and kidney are capable of FX - to -
NIV metabolism . -
pubmed
In this work , we successfully synthesized a large area of highly - crystalline
MoSe2 atomic layers on
SiO2 /
Si ,
mica and
Si substrates using a simple chemical vapour deposition ( CVD ) method at atmospheric pressure . -
pubmed
Atomic force microscopy (
AFM ) and Raman spectroscopy reveal that the as - grown ultrathin
MoSe2 layers change from a single layer to a few layers . -
pubmed
Photoluminescence (
PL ) spectroscopy demonstrates that while the multi - layer
MoSe2 shows weak emission peaks , the monolayer has a much stronger emission peak at ~ 1.56 eV , indicating the transition from an indirect to a direct bandgap . -
pubmed
Transmission electron microscopy (
TEM ) analysis confirms the single - crystallinity of
MoSe2 layers with a hexagonal structure . -
pubmed
Kirsten rat sarcoma viral oncogene (
KRAS ) mutations make some
tumours less responsive to treatment with biological therapies such as
cetuximab . -
pubmed
OBJECTIVES : To compare the performance and cost - effectiveness of
KRAS mutation tests in differentiating adults with metastatic
colorectal cancer whose
metastases are confined to the liver and are unresectable and who may benefit from first - line treatment with
cetuximab in combination with standard chemotherapy from those who should receive standard chemotherapy alone . -
pubmed
Two studies provided data on the accuracy of
KRAS mutation testing for predicting response to treatment in patients treated with
cetuximab plus standard chemotherapy . -
pubmed
Four RCTs provided data on the clinical effectiveness of
cetuximab plus standard chemotherapy compared with that of standard chemotherapy in patients with
KRAS wild - type
tumours . -
pubmed
Glufosinate ammonium dissipated rapidly in soil to
MPA in hours and gradually degraded to
MPP . -
pubmed
Poly ( epsilon - caprolactone ) nanofibers were surface modified with mussel inspired
DOPA - melanin ( DM ) coating for adsorption of
REST siRNA . -
pubmed
It was found that the extent of accumulation of
Ca , P ,
Zn , and
Na was not uniform throughout the
LAD artery and was higher in the proximal part than in the distal part with regard to the
LAD artery beneath the MB ( the tunneled
LAD artery ) . -
pubmed
The extent of accumulation of
Ca , P ,
Zn , and
Na in the proximal part of the tunneled
LAD artery was similar to that in the segments proximal to the MB , whereas the extent of accumulation of
Ca , P ,
Zn , and
Na in the distal part of the tunneled
LAD artery was similar to that in the segments distal to the MB . -
pubmed
We also identify a downregulation of miR-150 in
CD46 - costimulated T cells and identify the
glucose transporter 1 encoding transcript
SLC2A1 as a target of miR-150 regulation , connecting miR-150 with modulation of
glucose uptake . -
pubmed
OBJECTIVES : To evaluate the relevance of anti -
adalimumab ( anti -
ADA ) antibodies ( Abs ) and their relationship with clinical / laboratory features in
rheumatoid arthritis (
RA ) ,
ankylosing spondylitis (
AS ) and
psoriatic arthritis (
PsA ) . -
pubmed
Clinical / laboratory characteristics , disease activity , anti -
ADA , anti - nuclear ( ANA ) , anti - double strand ( ds)DNA , anti - extractable nuclear antigens (
anti - ENA ) and anti - phospholipid Abs ( aPL ) were evaluated at baseline , 4 , 12 and 24 weeks of
adalimumab treatment . -
pubmed
Anti -
ADA positivity was associated with significantly lower mean
adalimumab serum levels ( P<0.05 ) . -
pubmed
CONCLUSIONS : Anti -
ADA abs could be considered an early marker associated to a poor clinical response to
adalimumab treatment . -
pubmed
NJE effectively inhibited
2,2 '- azobis ( 2 - methylpropionamidine ) dihydrochloride (
AAPH)-induced oxidation of biomolecules analyzed by pBR322 plasmid DNA damage , protein oxidation of
bovine serum albumin and lipid peroxidation assays . -
pubmed
The
placenta growth factor (
PlGF ) is a member of the
vascular endothelial growth factor ( VEGF ) family , which shares with
VEGF - A the
tyrosine kinase receptor
VEGFR-1 and the co - receptor
neuropilin-1 (
NRP-1 ) . -
pubmed
Our findings emphasize the pivotal relevance of RNAi as an effective tool for increasing the therapeutic efficacy of
PMX , a cornerstone in the treatment regimens of
MPM , and thereby , raising the possibility for the development of a novel therapeutic strategy , combination therapy of
TS - shRNA and
PMX , that can surpass many of the currently applied , but less effective , therapeutic regimens against lethal
MPM . -
pubmed
Furthermore various hormones , including
leptin and
cortisone , are determined in cord plasma , and in heel prick blood
spots (
thyroxine ) . -
pubmed
Compared with the intakes of healthy children from National Nutritional Survey , the intake of
IBD group was lower for calories ( 78 % , P = 0.012 ) ,
carbohydrates ( 61 % P & lt ; 0.05 ) ,
magnesium ( 67 % P & lt ; 0.05 ) ,
vitamin C ( 34 % , P & lt ; 0.05 ) , and fiber ( 54 % , P & lt ; 0.05 ) and high for
B12 ( 141 % , P & lt ; 0.05 ) . -
pubmed
After
AVP - Vl receptor antagonist (
[ Deamino - penl , val4 , D - Arg8 ]- vasopressin ) was injected into
NTS or bilateral chemical damage to
NTS , the protective effect of the chemical stimulation of PVN on UC was also eliminated . -
pubmed
Momelotinib in
myelofibrosis :
JAK1 / 2 inhibitor with a role in treating and understanding the
anemia . -
pubmed
On MR , it was hypointense on
T1 , hyperintense on
T2 , and did not enhance with
gadolinium . -
pubmed
ERCC1 and
ERCC2 as predictive biomarkers to
oxaliplatin - based chemotherapy in
colorectal cancer patients from Egypt . -
pubmed
BACKGROUND : The impact of
Excision repair cross - complementation group 1 (
ERCC1 ) and group 2 (
ERCC2 ) expression levels on the efficacy of
oxaliplatin - based chemotherapy is still controversial . -
pubmed
The present study was conducted to determine the predictive value of these molecular biomarkers in stage III and
IV colorectal cancer (
CRC ) patients receiving
oxaliplatin ( OX)-based chemotherapy as first - line treatment . -
pubmed
CONCLUSIONS :
ERCC1 expression levels may help in selecting patients who benefit from
oxaliplatin chemotherapy in stage III & amp ;
IV CRC . -
pubmed
The clinical resolution rates in the PPS population at the test of cure ( TOC , primary efficacy end point , 7 - 30 days
post - therapy ) visit were 96.86 % ( 154/159 ) for
morinidazole and 96.73 % ( 148/153 ) for
ornidazole ( 95 % CI : -3.79 % to 4.03 % ) . -
pubmed
Vitamin D across
growth hormone ( GH ) disorders : From
GH deficiency to
GH excess . -
pubmed
The interplay between
vitamin D and the
growth hormone ( GH )/ insulin - like growth factor ( IGF )- I system is very complex and to date it is not fully understood . -
pubmed
GH directly regulates renal
1 alpha - hydroxylase activity , although the action of
GH in modulating
vitamin D metabolism may also be
IGF - I mediated . -
pubmed
On the other hand ,
vitamin D increases circulating
IGF - I and the
vitamin D deficiency should be normalized before measurement of
IGF - I concentrations to obtain reliable and unbiased
IGF - I values . -
pubmed
Indeed , linear growth after treatment of nutritional
vitamin D deficiency seems to be mediated through activation of the
GH / IGF - I axis and it suggests an important role of
vitamin D as a link between the proliferating cartilage cells of the growth plate and
GH /
IGF - I secretion . -
pubmed
Conversely , in active
acromegaly , a condition characterized by a chronic
GH excess , both increased and decreased
vitamin D levels have been highlighted , and the interplay between
vitamin D and the
GH / IGF - I axis becomes even more complicated when we consider the
acromegaly treatment , both medical and surgical . -
pubmed
The current review summarizes the available data on
vitamin D in the main disorders of the
GH /
IGF - I axis , providing an overview of the current state of the art . -
pubmed
Two types of SAMs with different dipole direction ,
thiophenol (
TP ) and
pentafluorobenzene thiol (
PFBT ) were employed , modifying the work function of the inkjet - printed
Ag ( 4.9 eV ) to 4.66 eV and 5.24 eV with
TP and
PFBT treatments , respectively . -
pubmed
The analytes were completely separated from the endogenous compounds on an Agilent Poroshell 120 SB -
C18 column ( 4.6mmx150 mm , 2.7mum ) using an isocratic elution with
methanol - 0.1 %
formic acid aqueous ( 4/1 , v / v ) as a mobile phase . -
pubmed
Positron emission tomography computed tomography ( PET -
CT ) scan of the lymph nodes revealed abnormal uptake of
fluorodeoxyglucose (
FDG ) . -
pubmed
The use of
MAPT was associated with similar mortality rate ( 5.9 % vs. 6.6 % ; RR = 0.92 ; 95 % CI 0.49 - 1.71 ; P = 0.68 ) and
stroke rate compared with
DAPT ( 1.3 % vs. 1.3 % ; RR 1.04 ; 95 % CI 0.27 to 4.04 ; P = 0.81 ) . -
pubmed
CONCLUSION :
MAPT use after TAVR is associated with lower rates of major
bleeding compared with
DAPT with no significant difference in mortality ,
stroke or
vascular complications . -
pubmed
[ Effects of
pargyline on histone methylation in promoter and enhancer regions and transcription of
CYP3A4/
3A7 ] . -
pubmed
This study was designed to investigate effects of
pargyline on
histone methylation in the promoter and enhancer regions and transcription of
cytochrome P450 3A4 / 3A7 (
CYP3A4/
3A7 ) gene . -
pubmed
The chromatin immunoprecipitation ( ChIP ) assay was performed with dimethylation of
histone H3 at
lysine 4 ( H3K4me2 ) , and
IgG antibodies respectively . -
pubmed
In conclusion , the enriched H3K4me2 in the promoter and enhancer regions was induced by
pargyline with
HNF4A or
GR binding site in
CYP3A4/
3A7 gene to activate the corresponding genes . -
pubmed
The total
nitrogen (
TN ) content in soil is an important index for soil fertility and the rapid prediction of
TN content with spectroscopy serves an important function in precision agriculture . -
pubmed
However , whether the
TN content in soil is predicted through its relationship with soil organic
carbon ( SOC ) or on its specific absorption is still debatable . -
pubmed
In order to explore the mechanism of reflectance spectroscopy for predicting total
nitrogen in soil , the changes of model accuracies and the correlation of
TN and SOC were analyzed . -
pubmed
Tacrolimus and Single Intraoperative High - dose of
Anti - T - lymphocyte Globulins Versus
Tacrolimus Monotherapy in Adult Liver Transplantation : One - year Results of an Investigator - driven Randomized Controlled Trial . -
pubmed
These excess
Mo atoms can then reorganize into closed , triangular
MTB - loops that can grow in size by adding more
Mo atoms to them . -
pubmed
Such
MTB networks have been observed in samples grown by molecular beam epitaxy ( MBE ) and consequently their presence needs to be considered in understanding the properties of MBE grown
MoSe2 and
MoTe2 . -
pubmed
INTRODUCTION : Once - weekly
semaglutide is a novel
glucagon - like peptide-1 (
GLP-1 ) analogue for the treatment of
type 2 diabetes that was associated with greater reductions in
glycated hemoglobin (
HbA1c ) and
body mass index (
BMI ) versus once - daily
GLP-1 analogue
liraglutide in a recent network meta - analysis (
NMA ) . -
pubmed
Influence of nonsynaptic
alpha1 glycine receptors on
ethanol consumption and place preference . -
pubmed
We examined whether intraoperative regional cerebral
oxygen saturation ( rSO2 ) and
neurological dysfunction prior to surgery , measured by robotic technology , are important predictors of
post - operative performance following CABG surgery . -
pubmed
DISCUSSION : Our results suggest that pre - operative performance is a stronger indicator of
post - operative neurological outcome than intraoperative rSO2 , and should be included as an important variable when elucidating the relationship between cerebral
oxygen levels and
post - operative
neurological impairment . -
pubmed
We found that those compounds slightly altered the expression of hNSC identity markers (
SOX2 ,
SOX3 and
NES ) , without impairing cell viability or proliferation , in part by either modulating
glycogen synthase kinase 3 beta (
GSK3beta ) signaling (
TBBPS ,
TCBPA and
BDE-47 ) , and slightly disturbing the NOTCH pathway (
TBBPA ,
TBBPS and
TCBPA ) . -
pubmed
Pinholin S2168 is a 68
amino acid integral membrane protein consisting of two transmembrane domains ( TMDs ) called TMD1 and TMD2 . -
pubmed
Systematic site - directed spin labeling and continuous wave electron paramagnetic resonance ( CW -
EPR ) spectroscopic studies of pinholin S2168 in
1,2 - dimyristoyl - sn - glycero-3 - phosphocholine (
DMPC ) proteoliposomes are used to reveal the structural topology and dynamic properties in a native - like environment . -
pubmed
Fabclavines are
NRPS - derived hexapeptides , which are connected by
PKS - derived
malonate units to an unusual
polyamine , also
PKS - derived . -
pubmed
In this study , we hypothesized that
heme oxygenase-1 (
HO-1 ) , an enzyme responsible for degradation of
heme to
carbon monoxide (
CO ) ,
bilirubin , and
iron is an important regulator of
inflammation and epithelial responses in the prostate . -
pubmed
Conversely , exogenous
CO ( 250 ppm ) increased
IL-1beta levels and suppressed cell proliferation in the prostates . -
pubmed
Interestingly , immunomodulatory effects of
HO-1 and
CO correlated with early induction of the
long - chain acyl - CoA synthetase 1 (
ACSL1 ) . -
pubmed
Therefore , we investigated the electrophysiological changes and the concomitant mechanical responses according to the expression levels of the
KCNJ2 E299V mutation during
sinus rhythm and
ventricular fibrillation . -
pubmed
We concluded that in both
sinus rhythm and
fibrillation , the
KCNJ2 E299V mutation results in very low contractility regardless of the expression level of mutation and increases the risk of
cardiac arrest and
cardiac death . -
pubmed
Role of
Chrysophanol in Epithelial - Mesenchymal Transition in
Oral Cancer Cell Lines via a
Wnt-3 - Dependent Pathway . -
pubmed
We further speculated that
chrysophanol - mediated EMT and
metastasis are involved in the
Wnt-3 - dependent signaling pathway . -
pubmed
The inhibition of the
EMT phenotype and
metastasis and accumulation of ROS caused by
chrysophanol was reversed by treatment with the
Wnt-3 agonist Bml 284 . -
pubmed
Therefore , our findings indicated that
chrysophanol altered
EMT formation , ROS accumulation , and
metastasis via the
Wnt-3 - dependent signaling pathway . -
pubmed
The endogenous production of
H2S is primarily mediated by
cystathione beta - synthase (
CBS ) ,
cystathione gamma - lyase (
CSE ) , and
3 - mercaptopyruvate sulfurtransferase (
3 - MST ) . -
pubmed
Finally , we show that identical neuropeptides were detected between tissues treated with 10 % NBF and an aqueous 1 % w / v
sodium phosphate solution ( composition of 10 % NBF without
formaldehyde ) , suggesting that utilizing a salt solution
wash affects neuropeptide detection and
formaldehyde does not affect neuropeptide detection when our
wash protocol is performed . -
pubmed
Also , the serum level of
interleukin-10 was increased after 3 days of
VitD supplementation ( p = 0.001 ) . -
pubmed
Baseline
integrase drug resistance mutations and conserved regions across HIV-1 clades in Cameroon : implications for transition to
dolutegravir in resource - limited settings . -
pubmed
BACKGROUND : Transition to
dolutegravir - based regimens in resource - limited settings (
RLS ) requires prior understanding of
HIV-1 integrase variants and conserved regions . -
pubmed
Of 288
HIV-1 integrase amino acid positions , 58.3 % were highly conserved across subtypes in the following major regions :
V75 - G82 , E85 -
P90 , H114 - G118 , K127 - W132 , E138 - G149 , Q168 - L172 , T174 - V180 , W235 - A239 and L241 - D253 . -
pubmed
Melatonin can be measured in different body fluids , such as
CSF , blood , saliva and urine . -
pubmed
Frozen ground muscle , when compared to unfrozen ground tissue , showed significantly lower
hexose monophosphates and significantly greater levels of all metabolites from
fructose diphosphate to
phosphoenolpyruvate , indicating activation of
phosphofructokinase and inhibition of
pyruvate kinase during the freezing process . -
pubmed
Thawing caused rapid glycolysis and the metabolites returned to near unfrozen levels by the time glycolysis ceased , due to strong activation of
phosphorylase by
Ca2 + . -
pubmed
After
sucrose gradient centrifugation , the densest subfraction ( sarcoplasmic reticulum ) contained the highest
( K + , Ca2 +)- ATPase activity and virtually no
( Na2 + , K +)- ATPase activity , even when latent (
Na + , K +)- ATPase activity was unmasked . -
pubmed
In addition , the sarcoplasmic reticulum fraction contained no significant
sialic acid ,
beta receptor binding activity , or
adenylate cyclase activity . -
pubmed
The results suggest that
( Na + , K +)- ATPase ,
sialic acid ,
beta - adrenergic receptors , and
adenylate cyclase can be entirely accounted for by the sarcolemmal content of cardiac microsomes . -
pubmed
Cyclic AMP stimulated phosphorylation by
[ gamma-32P ] ATP of two proteins of apparent Mr = 20,000 and 7,000 that were concentrated in sarcoplasmic reticulum , but the stimulation was markedly dependent on the presence of added soluble
cyclic AMP - dependent protein kinase . -
pubmed
Cyclic AMP also stimulated phosphorylation of membrane proteins in sarcolemma , but this phosphorylation was mediated by an endogenous
protein kinase activity . -
pubmed
In cirrhotics and in healthy controls the t 1/2 Leu was shortened during the additional
glucose administration compared with the t 1/2
Leu after
leucine administration alone and was prolonged by inhibiting
insulin secretion by
somatostatin . -
pubmed
Furthermore , subjects treated with an
ACE - inhibitor (
enalapril ) showed significant reduction in
acetylcholine - evoked axon reflex responses , while the test group of smokers showed a significant reduction in their dilator response to
pilocarpine.(ABSTRACT TRUNCATED
AT 400 WORDS ) -
pubmed
Normovolemic
hemodilution under normoxic conditions ( arterial
oxygen partial pressure normal ) at
rest is limited by the critical arterial
oxygen content of about 10 ml / dl corresponding to a
hemoglobin concentration of 7.5 g / dl . -
pubmed
Furthermore , stored blood ( blood bag ) seems to be of minor therapeutic effect because of the left shifted
oxygen content curve combined with a reduced effective
Hb concentration . -
pubmed
Tout2 but not
Tout1 was blocked by intracellular injection of 300 - 600 pmol ,
BaCl2 ( final concentration in the oocyte : 0.3 - 0.6 mM ) . -
pubmed
Injection of
KCl had no effect on either Tout1 or
Tout2 . -
pubmed
Tout2 but not
Tout1 was enhanced by low concentrations of
serotonin ( 0.5 - 2 nM ) . -
pubmed
Higher concentrations ( above 10 nM ) of
serotonin decreased the amplitude of
Tout2 . -
pubmed
The effect of
serotonin was blocked by the
protein kinase inhibitor , H-7 ( 25 microM ) . -
pubmed
Tout2 but not
Tout1 was enhanced by 10 nM -
phorbol myristate acetate . -
pubmed
Higher concentrations of the
phorbol ester decreased the amplitude of
Tout2 . -
pubmed
This current reflects a second process of
chloride channel opening which can be enhanced by
serotonin via activation of
protein kinase C. -
pubmed
Influence of
monosaccharides on the fibrillogenesis of
type I collagen . -
pubmed
The in vitro self - assembly of
Type I rat tail tendon collagen in the presence of
monosaccharides was studied turbidimetrically . -
pubmed
The molecular mass of this membrane - bound adrenal
trypsin - like enzyme (
mATLE ) is 31 kDa as determined by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis , and the enzyme is extremely basic , binding to
carboxymethyl - Sephadex at pH 8.5 . -
pubmed
The pH optimum of
mATLE using
t - butoxycarbonyl - Glu - Lys - Lys - aminomethylcoumarin as a substrate is 8.5 - 8.7 , and its Km value for this substrate is 2.2 mM. -
pubmed
Sequencing of cleavage products released from Peptide B revealed that the
enzyme preferentially cleaves between and following the paired
basic residues at positions 23 and 24 of Peptide B ( thus generating
[ Met - enkephalin ]- Arg - Phe and
Arg -[ Met - enkephalin ]- Arg - Phe ) . -
pubmed
The phenotype of the common , immature thymocyte was identified by the binding of either
fluorescein isothiocyanate (
FITC)- or
phycoerythrin ( PE)-conjugated monoclonal anti - T6 (
OKT 6 ) antibody and of mature thymocytes by the binding of
monoclonal anti - T3 (
OKT 3 ) antibody . -
pubmed
Plasma total ,
high - density - lipoprotein (
HDL ) ,
low - density - lipoprotein and
very - low - density - lipoprotein cholesterol and
triglycerides were determined weekly . -
pubmed
A significant effect ( P less than 0.05 ) of dietary fat on plasma total and
HDL cholesterol was observed between the end of wk 2 and 7 . -
pubmed
Low fat intake resulted in 17 + /- 2 mg / dL lower total
cholesterol and 10 + /- 1 mg / dL lower
HDL cholesterol than the usual fat intake . -
pubmed
Intracellular
calcium release mediated by two
muscarinic receptor subtypes . -
pubmed
By combining fluorescent indicator dye (
fura-2 ) studies with electrophysiological measurements it is shown that stimulation of
mAChR I and
mAChR III readily leads to release of
calcium from intracellular stores and to associated conductance changes , whereas stimulation of
mAChR II and
mAChR IV exerts no such effect . -
pubmed
Dose - response curves describing the amplitude or the delay of the
calcium rise induced by
acetylcholine suggest that the apparent affinity of
mAChR III for its agonist is higher by about one order of magnitude than that of
mAChR I. -
pubmed
Thiol /
disulfide exchange between
3 - hydroxy-3 - methylglutaryl - CoA reductase and
glutathione . -
pubmed
At pH 7.0 , 37 degrees C , the second order rate constant for inactivation of the reduced
enzyme by
GSSG is 1700 + /- 200 M-1 min-1 , approximately 20 - fold faster than the reaction of
GSSG with a typical , unhindered
thiol of pKa 7.7 . -
pubmed
High concentrations of
GSH or lower concentrations of
dithiothreitol restore the activity of the oxidized
enzyme . -
pubmed
The oxidation of the
enzyme by
GSSG is only 30 - fold slower in the presence of saturating levels of both substrates . -
pubmed
The incomplete inhibition of
thiol /
disulfide exchange by substrates can lead to significant changes in the activity of the
enzyme during the assay when
glutathione is present . -
pubmed
At redox equilibrium , both in the absence and presence of substrates , the activity of the
enzyme depends on the quantity [
GSH]2/[
GSSG ] , suggesting that the redox transition involves the formation of a
protein - SS - protein
disulfide . -
pubmed
Thus ,
HMG - CoA reductase is very sensitive to
dithiol oxidation both kinetically and thermodynamically . -
pubmed
Significant changes in the oxidation state and activity of this
enzyme could be expected to result from normal changes in the
thiol /
disulfide oxidation state of the cellular
glutathione redox buffer . -
pubmed
Role of iodinated hormones ,
parathyroid hormone , calcitonin ,
vitamin D and blood
calcium levels ] . -
pubmed
The decay rate of the excited triplet state of
Zn cytochrome c was enhanced by electron acceptors including
methyl viologen and
ferric complexes of
cyanide ,
oxalate ,
EDTA and
cytochrome c at room temperature . -
pubmed
After incubation of the cells to 1 microM
( 3H ) 7 - OH - MTX , four radiolabelled peaks , representing 75 % of the intracellular
3H , were converted to
7 - OH - MTX upon exposure to
hog kidney conjugase indicating the formation of
7 - OH - MTX polyglutamyl derivatives . -
pubmed
Change and correlates of change in high and
low density lipoprotein cholesterol after six years : a prospective study . -
pubmed
Change and correlates of change in high and
low density lipoprotein cholesterol (
HDL and
LDL ) were studied in a prospective population - based study of 614 men and women in Rancho Bernardo , CA , 1972 - 1980 . -
pubmed
Among the behavioral variables ,
HDL change was positively associated with change in
alcohol use in both sexes and with change in postmenopausal
estrogen use in women and was inversely associated with change in
obesity index in men . -
pubmed
Effect of pretreatment of animals with
ethidium bromide , an intercalating dye which has been shown to prevent the in vitro nuclear binding of
steroid hormone - receptor complexes , on the depletion of receptor induced by
testosterone administration was also insignificant . -
pubmed
This drug does not stimulate
insulin release but works by lowering
glucose in peripheral tissues . -
pubmed
Interferon alpha induces rapid
tyrosine phosphorylation of the
vav proto - oncogene product in hematopoietic cells . -
pubmed
The
IFN alpha - induced
tyrosine phosphorylation of
p95vav was time- and dose - dependent , confirming the specificity of the process . -
pubmed
After
IFN alpha stimulation significant amounts of phosphorylation of
p95vav on
tyrosine residues were detectable . -
pubmed
Monoaldehyde allosteric effectors of
hemoglobin were designed , using molecular modeling software ( GRID ) , to form a
Schiff base adduct with the
Val 1 alpha N - terminal
nitrogens and interact via a salt bridge with
Arg 141 alpha of the opposite subunit . -
pubmed
It was envisioned that the molecules , which are
aldehyde acids , would produce a high - affinity
hemoglobin with potential interest as antisickling agents similar to other
aldehyde acids reported earlier . -
pubmed
However ,
oxygen equilibrium curves run on solutions obtained from T- ( tense ) state
hemoglobin crystals of reacted effector molecules produced low - affinity
hemoglobins . -
pubmed
The
GABA - A agonist
muscimol , but not
MK-801 , was effective if given 30 , 45 , or 60 min after embolization ( potency ratio compared with saline of 3.0 , 2.3 , 1.8 , respectively ) . -
pubmed
Agonists of
GABA - A , but not
GABA - B , receptors blocked the toxic vacuolization seen in the cingulate and retrosplenial cortex after
MK-801 treatment . -
pubmed
Both sets of phosphopeptides contained candidate
MAPK target sites at
Thr292 and
Thr386 that fit the consensus sequence ProXThr*
Pro . -
pubmed
Replacement of either
Thr292 or
Thr386 with
alanine by site - directed mutagenesis reduced the
phosphate incorporation respectively to 32 or 75 % that of
wild type MKK . -
pubmed
We conclude that the phosphorylation of
MKK at
Thr292 and
Thr386 does not interfere with catalysis in vitro . -
pubmed
Other receptors that stimulate
APPs secretion include those for
bradykinin ,
vasopressin , and
interleukin-1 receptors . -
pubmed
This increase is
tetrodotoxin - sensitive and frequency - dependent , suggesting that
APPs release may normally depend on neuronal activity . -
pubmed
For
2 - , 3 - , 4 - , and
6 - fluorodeoxyglucose analogs , the alpha anomers permeate more rapidly , and the
P alpha / P beta ratio is dependent on the position of fluorination , with values of 1.1 , 1.3 , 2.5 , and 1.6 , respectively , obtained at 37 degrees C. These results have been analyzed in terms of a simple alternating conformation model for the
glucose transporter . -
pubmed
Progesterone has been implicated to play a critical role in mediating
LH induction of ovulation and possibly luteinization . -
pubmed
Transcripts of approximately 11.0 , 7.2 , 6.8 , 6.2 , 3.4 , and 3.1 kilobases in size were induced by ovulatory ( 500 ng / ml ) , but not low ( 50 ng / ml ) , concentrations of
LH and
FSH as well as by
forskolin ( 10 microM ) ,
GnRH ( 1 microM ) , and
phorbol 12 - myristate 13 - acetate ( 200 nM ) . -
pubmed
The antiprogestins
RU486 and
ZK98299 did not inhibit induction of
PR mRNA and protein , but effectively blocked agonist activation of
glucocorticoid response element2 - E1b -
chloramphenicol acetyltransferase , as well as
LH stimulation of luteinization in vitro . -
pubmed
These results provide direct evidence that agonists stimulating diverse intracellular pathways can induce
PR in granulosa cells , that
progesterone plays a functional role in the luteinization process triggered by the
LH surge , and that the effects are mediated at least in part by induction of
PR . -
pubmed
Unlike other
fructose using tissues , where
Glut-5 may subserve the role of a
fructose carrier , in brain where
fructose is not used as a substrate ,
Glut-5 may transport
glucose alone . -
pubmed
All the doses of
Amitriptyline produced significant
hyperglycemia at 4 h , which attained a peak at 24 h with 16 mg / kg dose and appears to be due to blockade of the uptake of monominergic transmitters across the axoplasmic membrane , It ( 16 mg / kg ) also produced
glucose intolerance during early hours probably due to interference with
gastrin function . -
pubmed
The
amino acid sequence of the deduced peptide showed a typical
flavin adenine dinucleotide - binding site in its N - terminal region , confirming the flavoproteinic structure of
delta 4 ( 5 alpha ) DH . -
pubmed
The involvement of
catecholamines , in
hypertension ,
alcohol and
ACE - inhibition . -
pubmed
We have attempted to provide a progress report on current research on the role of
catecholamines and
serotonin receptor subtypes in feeding control . -
pubmed
Chick cDNAs identified included : the
parathyroid hormone - related protein , an immediate early gene ; the delta - subunit of the neuronal - specific
Ca2 +/ calmodulin - regulated protein kinase II ; and the
GTP - binding protein
CDC42 , a member of the
ras superfamily of G proteins . -
pubmed
These results indicate the potential involvement of both
Ca2 +/calmodulin - mediated signaling and
GTPase cascades in the response to noise damage and during hair cell regeneration in the chick basilar papilla . -
pubmed
It is metabolized by the
cytochrome P-450 system to a stable , pharmacologically active
epoxide intermediate ,
carbamazepine-10 , 11 - epoxide . -
pubmed
Comparative studies of the phage
T2 and
T4 DNA ( N6 - adenine ) methyltransferases :
amino acid changes that affect catalytic activity . -
pubmed
Kinetic analyses with purified homogeneous enzymes showed that the two
MTases had similar Km values for the
methyl donor ,
S - adenosyl - L - methionine , and for substrate DNA . -
pubmed
Since the
T2 and T4
MTases differ at only three
amino acid residues ( at positions 20 [ T4 ,
Ser ;
T2 ,
Pro ] , 26 [ T4 ,
Asn ;
T2 ,
Asp ] , and 188 [ T4 ,
Asp ;
T2 ,
Glu ] ) , we have produced hybrid proteins to determine which residue(s ) is responsible for increased catalytic activity . -
pubmed
BACKGROUND : A synthetic peptide corresponding to residues 75 - 84 of the alpha1 domain of
HLA - B7 molecule (
HLA - B7.75 - 84 [ Allotrap ] ) inhibits cytotoxic T cell function in vitro and , when combined with subtherapeutic doses of
cyclosporine (
CsA ) , prolongs allogeneic cardiac survival . -
pubmed
alone or with
CsA plus alternate - day
HLA - B7.75 - 84 ( 20 mg / kg / day i.p . ) -
pubmed
RESULTS :
HLA - B7.75 - 84 plus
CsA prolonged allograft survival ( 75 % of allografts survived & gt;90 days ) compared with
CsA alone ( 27 % of allografts survived & gt;90 days ) ( P<0.05 ) . -
pubmed
Results suggest that the estrus - inducing action of
LHRH ,
PGE2 and db
cAMP occurs through the activation of the
progesterone receptor . -
pubmed
Proton release upon
glutathione binding to
glutathione transferase P1 - 1 : kinetic analysis of a multistep
glutathione binding process . -
pubmed
The fate of the
thiol proton coming from the ionization of the
sulfhydryl group of
GSH in the active site of
glutathione transferase P1 - 1 has been studied . -
pubmed
pH changes caused by the binding of
GSH to the
enzyme in the absence of any inorganic buffer indicate that the
thiol proton leaves the active site when the binary complex is formed . -
pubmed
By comparing the kinetic behavior of three distinct events occurring during the binding of
GSH to the
enzyme , i. e. , proton release , ionization of bound
GSH and quenching of intrinsic fluorescence , it appears that the binding process follows a multistep mechanism possibly involving the conformational transition of a weak precomplex into the final Michaelis complex . -
pubmed
Immunohistochemical analysis with monoclonal antibodies to
bcl-2 oncoprotein in
formalin - fixed paraffin - embedded tissue sections revealed that severe
epithelial dysplasias had a higher percentage of immunoreactivity than did mild and moderate
dysplasias and
squamous cell carcinomas . -
pubmed
Interactions with the promoter for the
flagellin gene
fliC were analyzed using
DNase I and
KMnO4 footprinting over a range of temperatures . -
pubmed
In contrast to the previously published
hMUC3 sequence , which terminates shortly after a single
cysteine - rich
EGF - like domain , conceptual protein translation of the dominant and largest PCR product identified two extracellular
cysteine - rich
EGF - like domains separated by an N - glycosylation - rich domain and a potential coiled -
coil region , followed by a putative transmembrane region and a 75
amino acid cytoplasmic tail . -
pubmed
Roles of
his205 ,
his296 ,
his303 and
Asp259 in catalysis by
NAD +- specific D - lactate dehydrogenase . -
pubmed
The role of three
histidine residues (
His205 ,
His296 and
His303 ) and
Asp259 , important for the catalysis of
NAD +- specific D - lactate dehydrogenase , was investigated using site - directed mutagenesis . -
pubmed
None of these residues is presumed to be involved in coenzyme binding because Km for
NADH remained essentially unchanged for all the mutant
enzymes . -
pubmed
These analyses revealed that the gene encoding
Dab2 , a
mitogen - responsive phosphoprotein , is differentially expressed in wild - type and
GATA-6 - deficient ES cells . -
pubmed
Taken together , these data demonstrate that the
mitogen - responsive phosphoprotein
Dab2 is a downstream target of
GATA-6 in the visceral endoderm . -
pubmed
Characterization of the new
alpha-1 - antitrypsin - deficient
PI M - type allele ,
PI M(vall d'hebron ) ( Pro(369)-->
Ser ) . -
pubmed
RESULTS : Regarding
acute phase response , the EAAA and
OAAA groups showed significant increases in
C - reactive protein ( P & lt;.001 and P = .001 ) , body temperature ( P = .035 and P = .048 ) , and leukocyte count ( P & lt;.001 and P & lt;.001 ) . -
pubmed
Regarding complement activation , the
C3d / C3 ratio disclosed significant postoperative elevations in the EAAA and
OAAA groups ( P = .013 and P = .009 ) . -
pubmed
Meanwhile ,
ATP - induced DNA fragmentation was fully blocked by pretreatment with
oxidized ATP (
OxATP ) , a compound recently shown to block
P2z receptors . -
pubmed
CONCLUSION : These data indicate that
P2z receptors on lymphocytes play an important role in apoptosis induced by
ATP in vitro . -
pubmed
The
protein is examined in powder form , in
D ( 2 ) O , and in four two - component perdeuterated single - phase cryosolvents in which it is active and stable . -
pubmed
The dynamic transition in all of the mixed cryosolvent -
protein systems is much more gradual than in pure
D ( 2 ) O , consistent with a distribution of energy barriers . -
pubmed
GM - CSF regulates
bleomycin - induced
pulmonary fibrosis via a
prostaglandin - dependent mechanism . -
pubmed
Exogenous addition of
GM - CSF to alveolar macrophages reversed the
PGE2 synthesis defect in vitro . -
pubmed
The enzymes involved in the production of the precursors of
carbamyl phosphate and
nitrogen assimilation ,
glutamine synthetase and
nitrate reductase , were also investigated , and it appears that these two enzymes are present in the bacteria . -
pubmed
The radiolabeled
DAT ligand
[ 3H ] GBR12935 was bound to peripheral lymphocytes in a manner consistent with the specific binding to a
dopamine uptake system , with a dissociation constant similar to that found in striatum , but with a lower density of binding sites . -
pubmed
Western blot analysis using antibodies raised against
amino or carboxy terminus of
DAT or against
VMAT-1 or
VMAT-2 revealed labeling of single bands of approximately 76 , 55 or 68 KDa , respectively , displaying similar migration characteristics in lymphocytes and test tissues used for comparison . -
pubmed
Immunofluorescence revealed that anti -
dopamine , anti -
tyrosine hydroxylase , anti -
DAT , anti -
VMAT-1 and anti -
VMAT-2 antibodies labeled the total population of cytospin - centrifuged lymphocytes mounted on microscope slides . -
pubmed
Confocal laser microscopy demonstrated that
dopamine and
VMAT-2 immunoreactivity was developed mainly in cytoplasmic punctiform areas likely corresponding to vesicles and to a lower extent was associated to plasma membrane . -
pubmed
Lymphocyte DAT characterized in this study has probably functional relevance as
[ 3H ] dopamine was taken up by intact lymphocytes and uptake was inhibited specifically by compounds known to affect
dopamine transport . -
pubmed
Although this therapy has been traditionally performed by oral
estrogen or bilateral orchiectomy , there are now two most important pharmacological hormonal therapies :
LH - RH agonist and antiandrogen therapy . -
pubmed
Pain associated with injection of
botulinum A exotoxin reconstituted using isotonic
sodium chloride with and without preservative : a double - blind , randomized controlled trial . -
pubmed
Wortmannin , a specific inhibitor of
phosphatidylinositol 3 - kinase (
PI3 K ) , mimicked the priming irradiation in that the inhibitor alone rendered the cells resistant against the induction of
chromosome aberrations and apoptosis by the subsequent X - ray irradiation . -
pubmed
Sonochemiluminescence (
SCL ) of
luminol due to a single bubble is studied through spectral measurement . -
pubmed
The first receptor model is a self training factor analysis (
FA ) model with non - negative constraints that was applied to identify the
PCB sources and significant congener patterns . -
pubmed
The
FA model indicated two significant factors , the major one being
Aroclor 1242 and the other , a profile dominated by low chlorinated congeners , indicating a possible
PCB alteration profile . -
pubmed
It was also deduced from the
FA model that very small contributions of more highly chlorinated
Aroclors may be present in the system . -
pubmed
Here we apply extended X - ray absorption fine structure ( EXAFS ) measurements to characterize the metal ion binding site , in frozen solution , of the unimolecular quadruplex formed by the
thrombin binding aptamer , d(G(2)T(2)G(2)TGTG(2)T(2)G(2 ) ) (
TBA ) , in the presence of
Pb ( 2 + ) ions . -
pubmed
The
Pb L ( III ) -edge X - ray absorption spectrum of this metal - DNA complex is very similar to that we obtain for a
Pb ( 2 +)-stabilized quartet system of known structure constructed from a modified
guanine nucleoside (
G1 ) . -
pubmed
The Fourier transforms of the
Pb ( 2 + ) complexes with both
TBA and
G1 show a first - shell interaction at about 2.6 A , and a weaker , broader shell at 3.5 - 4.0 A. Quantitative analysis of the EXAFS data reveals the following : ( i ) very close agreement between interatomic distances at the metal coordination site for the
Pb ( 2 +)- G1 complex determined by EXAFS and by X - ray crystallography ; ( ii ) similarly close agreement between interatomic distances measured by EXAFS for the
Pb ( 2 +)- G1 and
Pb ( 2 +)- TBA complexes . -
pubmed
These results provide strong evidence for binding of the
Pb ( 2 + ) ion in the region between the two quartets in the
Pb ( 2 +)- TBA complex , coordinated to the eight surrounding
guanine O6 atoms . -
pubmed
RESULTS : Cumulative oxidation of
( 13 ) C - oleate at 11.5 h postdose was significantly greater during the heavy exercise ( 49 + /- 4 % ) trial than both the light exercise ( 39 + /- 4 % ) and the
rest trials ( 34 + /- 4 % ) ( P & lt ; 0.005 ) . -
pubmed
Oxidation of
d ( 31 )- palmitate recovery at 11.5 h postdose was not different during the heavy exercise ( 12 + /- 1 % ) , light exercise ( 11 + /- 1 % ) , and
rest trials ( 10 + /- 1 % ) . -
pubmed
Culture of G(0 )
CD34(+ ) cells isolated based on
Hoechst 33342 /
PyroninY staining with
Tpo ,
SCF and
FL for 48 hrs , results in significantly elevated
Survivin mRNA and protein levels . -
pubmed
Moreover , labeling of fresh G(0 )
CD34(+ ) cells with
5 -( and 6 - ) carboxyfluorescein diacetate succinimidyl ester (
CFSE ) before culture with growth factors for up to 72 hrs , revealed that
Survivin expression was elevated in CFSE(bright ) G(0 )
CD34(+ ) cells , indicating that up - regulation occurred before entry into
G1 . -
pubmed
In addition ,
G - Rh ( 2 ) almost completely inhibited
telomerase activity , as measured by
polymerase chain reaction - based telomeric repeat amplification protocol coupled with enzyme - linked immune sorbent assay , and
human telomerase reverse transcriptase mRNA . -
pubmed
MATERIAL / METHODS : Postmortem
infarct volume was determined by quantitative image analysis of
triphenyltetrazolium - stained brain sections , collected at 1 h , 3 h and 24 h after initiation of
cerebral ischemia , to measure consistent
infarct volumes of the
post -ischemic gerbil brain . -
pubmed
Acute administration of
clonidine ( 2 mg / kg ) , an
alpha2AR agonist , elicited dramatic decreases in DNA synthesis in the forebrain , brainstem , and cerebellum whether given on gestational day ( GD ) 21 , or on postnatal days (
PN ) 1 or 8 . -
pubmed
However ,
alpha2AR blockade elicited by
yohimbine ( 2.5 mg / kg ) also resulted in decreased DNA synthesis on GD21 and
PN8 , albeit to a smaller extent than with
clonidine . -
pubmed
Because
betaARs are also known to regulate DNA synthesis , we used
propranolol ( 10 mg / kg ) blockade of
betaARs to evaluate whether the alpha2AR effects were mediated by presynaptic
autoreceptors that regulate the release of
norepinephrine and consequent
betaAR responses ; the effects of
yohimbine were still discernible in the presence of
propranolol . -
pubmed
Although the role of
PTP-1B in dephosphorylating various
cell surface receptor tyrosine kinases is clear , the mechanisms by which it modulates receptor function from the endoplasmic reticulum (
ER ) remains an enigma . -
pubmed
AG490 , a
tyrosine kinase inhibitor affecting
Jak proteins , inhibits
CD2 - mediated
IFN - gamma mRNA expression , secretion , and nucleoprotein binding to the
IFN - gamma
STAT5 site in a dose - dependent fashion . -
pubmed
Dodecanethiol MPNs , in which the monolayer is
dodecanethiol linked to the gold nanoparticle , have shown great promise as a
GC stationary phase with efficient columns having been produced in a variety of capillary i.d . -
pubmed
The efficiency of the
dodecanethiol MPN stationary phase was determined with a 100 microm i.d . -
pubmed
Areas of application illustrated and discussed utilizing the
dodecanethiol MPN stationary phase include complementary separations such as two - dimensional
GC (
GC x
GC ) , potential utilization within a model system for a micro - fabricated
GC ( microGC ) , as well as efficient single dimension high - speed separations . -
pubmed
Included is a selectivity comparison of the retention behavior of the
4 - chlorobenzenethiol MPN stationary phase and the
dodecanethiol MPN stationary phase . -
pubmed
The anti -
albuterol serum was treated with saturated
ammonium sulfate solution to obtain the antibody (
immune globulin ) . -
pubmed
The results showed that the
immune globulin - affinity column has the characteristic of high purity and high specificity , and it is suitable for the preparation of chromatographic plasma specimen to determine
albuterol in plasma . -
pubmed
PURPOSE :
Fructose 2,6 - bisphosphate (
F2,6BP ) is a potent activator of
phosphofructokinase , which is a rate - limiting enzyme of glycolysis . -
pubmed
The concentration of
F2,6BP depends on the activity of the bifunctional enzyme ,
6 - phosphofructo-2 - kinase / fructose-2,6 - bisphosphatase (
PFK-2 /
FBPase ) . -
pubmed
Quantitative , real - time PCR analysis confirmed the overexpression of
PFKFB3 mRNA in
colon carcinoma , with the dominant variant being the
PFKFB3 -
ACG isoform that contains a phosphorylation site at
Ser461 . -
pubmed
Roles of the
threonine 407 ,
aspartic acid 417 , and
threonine 419 residues in
P450 2B1 in metabolism . -
pubmed
We have previously observed that the quadruple ( S407T - N417D - A419T - K473 M ) and triple ( S407T - N17D - A419 T ) mutants of the chimeric construct of
P450 2B1 / 2B2 do not undergo mechanism - based inactivation by
17alpha - ethynylestradiol (
17EE ) and
tert - butyl 1 - methyl-2 - propynyl ether (
tBMP ) . -
pubmed
In the presence of the alternate oxidants
cumene hydroperoxide and
tert - butyl hydroperoxide , the wild - type
2B1 was not inactivated by
17EE . -
pubmed
Metabolism of
17EE by
2B1 supported by these alternate oxidants revealed differences in the
metabolites that may be related to the inability of
2B1 to be inactivated under these conditions . -
pubmed
Apoptosis was detected by means of
annexin V and
propidium iodide staining . -
pubmed
SCF synergized with chemoattractants in causing basophil upregulation of the
integrin CD11b , and this effect was inhibited by a
c - kit antibody , the
tyrosine kinase inhibitor
imatinib mesylate (
STI-571 ) , and a
phosphatidylinositol 3 kinase inhibitor but not by inhibitors of
p38 mitogen - activated protein kinase or
mitogen - activated protein kinase /
extracellular signal - regulated kinase kinase . -
pubmed
Thiazolo [ 5,4 - f ] quinazolin-9 - ones , inhibitors of
glycogen synthase kinase-3 . -
pubmed
In an effort to identify new
protein kinase inhibitors with increased potency and selectivity , we have developed the microwave - assisted synthesis of
thiazolo [ 5,4 - f ] quinazolin-9 - ones . -
pubmed
Molecular modeling studies suggest that the most selective
GSK-3 inhibitors 7a - d bind into the
ATP - binding site through a key
hydrogen bond interaction with
Val135 and target the specific hydrophobic backpocket of the enzyme . -
pubmed
The thermal behavior of
polyester -
polyurethane and
polyether - polyurethane in air atmosphere was investigated by using FTIR analysis in the temperature range from 20 to 400 degrees C. The thermal degradation behaviors of
polyester -
polyurethane and
polyether - polyurethane in different atmospheres were investigated by using thermogravimetry analysis (
TG ) in the temperature range from 20 to 700 degrees C. The FTIR results together with the
TG investigations showed that
polyester -
polyurethane has two degradation steps in air , but
polyether - polyurethane has only one degradation step . -
pubmed
It is therefore assumed that the supraordinated hormones ,
GnRH ,
FSH , and/or
LH , have an effect on the urodynamic parameters . -
pubmed
Within 1 week of
GnRH analogue administration , plasma
FSH and
LH levels decreased from 72.5 and 7.7 to 7.75 and 0.72ng / mL , respectively . -
pubmed
The improvement in bladder function after the application of
GnRH - application is presumably a direct effect of the
GnRH as a relationship between the plasma
gonadotropin levels and the urodynamic parameters could not demonstrated . -
pubmed
Aggregated
silver nanoplates can serve as active
SERS substrates with an enhancement factor of about 4.5 x 10(5 ) using
2 - aminothiophenol as a probing molecule . -
pubmed
RESULTS : The seropositive rates for
HBsAg ,
anti - HBs , and
anti - HBc were 1.2 % , 50.5 % , and 3.7 % , respectively , in those born after the vaccination program ( & lt;20 years of age ) in 2004 . -
pubmed
polygyrus were resistant to
trinitrobenzenesulfonic acid (
TNBS)-induced
colitis , a Th1
cytokine - dependent
inflammation . -
pubmed
Furthermore , an association study was performed , testing three single
nucleotide polymorphisms ( SNPs ) of
suppressor of cytokine signalling 3 (
SOCS3 ) in 162
NR and 184 SVR subjects (
SOCS3 -8464 A / C ( rs12952093 ) , -4874 A / G ( rs4969170 ) and 1383 A / G , ( rs4969168 ) ) . -
pubmed
Of critical importance to sustainable agriculture are the symbioses formed between more than 80 % of terrestrial plants and arbuscular mycorrhizal (
AM ) fungi and between legumes and
nitrogen - fixing rhizobial bacteria . -
pubmed
MtDMI3 encodes a
Ca ( 2 +)/ calmodulin - dependent protein kinase (
CCaMK ) essential for the transduction of the
Ca ( 2 + ) signal induced by the perception of
Nod factors . -
pubmed
Use of
aromatase inhibitors rather than
tamoxifen for
breast cancer will likely lead to more
osteoporosis . -
pubmed
STM results indicated that the symmetry of ordered structures decreased as the
alkyl chain became longer , with the exception of
C ( 6 ) OPc , which preferentially formed a quasi-3 - fold symmetrical structure . -
pubmed
We studied the potential of systemically administered
aminoglycosides as a therapy for
retinal degeneration resulting from premature termination codon (
PTC ) mutations . -
pubmed
These experiments indicated that
gentamicin treatment induced on average a 5.3 % extra read - through of the S334ter
PTC in vitro , but did not affect the rd12
PTC . -
pubmed
Two different chitosan (
CS ) nanocarriers namely nanoparticles and nanoemulsion were developed to prolong
Indomethacin ( IM ) precorneal residence time and to improve its ocular bioavailability the main limitations in its management of
post - operative
inflammation and
intraocular irritation after
cataract extraction . -
pubmed
CS - nanoparticles were developed by modified ionic gelation of
CS with
tripolyphosphate while nanoemulsion was prepared by spontaneous emulsification technique . -
pubmed
H2O2 - dependent hyperoxidation of
peroxiredoxin 6 (
Prdx6 ) plays a role in cellular toxicity via up - regulation of
iPLA2 activity . -
pubmed
Peroxiredoxin 6 (
Prdx6 ) is a bifunctional enzyme with
peroxidase activity and
Ca2 + -independent
phospholipase A2 (
iPLA2 ) activity . -
pubmed
Here , we report that
H2O2 - induced
cellular toxicity acts through
Prdx6 hyperoxidation . -
pubmed
Under high concentrations of
H2O2 ( & gt ; 100 microm ) ,
Prdx6 , and
2 - Cys Prdxs were hyperoxidized . -
pubmed
Contrary to hyperoxidation of
2 - Cys Prdxs , hyperoxidation of
Prdx6 was irreversible in vivo . -
pubmed
Surprisingly ,
H2O2 - induced cell cycle arrest at the
G2 / M transition correlated with hyperoxidation and increased
iPLA2 activity of
Prdx6 . -
pubmed
Furthermore , the
H2O2 - mediated increase in
iPLA2 activity was dramatically abolished in a hyperoxidation mutant ( C47A ) , an
iPLA2 mutant ( S32A ) , and a double mutant ( C47A / S32A ) of
Prdx6 , demonstrating the essential requirement of
Prdx6 C47 hyperoxidation for its
iPLA2 activity . -
pubmed
Together , our results demonstrate that
H2O2 - mediated hyperoxidation of
Prdx6 induces cell cycle arrest at the
G2 / M transition through up - regulation of
iPLA2 activity . -
pubmed
Desflurane but not
sevoflurane can increase lung resistance via
tachykinin pathways . -
pubmed
Antagonization of
tachykinin receptors of airway smooth muscles completely diminished the increase in R(L ) induced by
desflurane . -
pubmed
Here , we show that exposing DT104 to
mitomycin C , a DNA - damaging agent , induced production of prophage - encoded
ArtA /
ArtB. -
pubmed
Pertussis - sensitive
G proteins were labelled in the presence of
[ ( 32 ) P ] NAD and
ArtA , and the label was released by
HgCl ( 2 ) , which is known to cleave
cysteine -
ADP -
ribose bonds . -
pubmed
ADP - dependent modification of
G proteins was markedly reduced in in vitro - synthesized
ArtA(6Arg - Ala ) and
ArtA(115Glu - Ala ) , in which
alanine was substituted for the conserved
arginine at position 6 ( necessary for
NAD binding ) and the predicted catalytic
glutamate at position 115 , respectively . -
pubmed
Hydrogen peroxide , an oxidative stressor , also induced
ArtA /
ArtB production , suggesting that these agents induce in vivo synthesis of
ArtA /
ArtB. -
pubmed
These results , taken together , suggest that
ArtA /
ArtB is an active toxin similar to
PTX . -
pubmed
Tissue inhibitor of matrix metalloproteinase-1 reduces
phosphatidylserine exposure on activated and aged platelets . -
pubmed
The modulation transfer function (
MTF ) of the system was determined from simulated images of a point source of
( 99m ) Tc , positioned at different depths in a water phantom . -
pubmed
Electrophysiological data have shown that the acidic residues E106 in transmembrane helix 1 ( TM1 ) and E190 in
TM3 contribute to the high selectivity of
ORAI1 channels for
Ca ( 2 + ) . -
pubmed
We have examined the pore architecture of the
ORAI1 channel using
ORAI1 proteins engineered to contain either one or two
cysteine residues . -
pubmed
Disulfide cross - linking shows that
ORAI1 assembles as a tetramer or a higher oligomer with TM1 centrally located . -
pubmed
Cysteine side chains projecting from TM1 at position 88 , 95 , 102 , or 106 cross - link efficiently to the corresponding side chain in a second
ORAI1 monomer . -
pubmed
Cysteine residues at position 190 or at surrounding positions in
TM3 do not cross - link . -
pubmed
We conclude that E106 residues in wild - type
ORAI1 are positioned to form a
Ca ( 2 + ) binding site in the channel pore and that E190 interacts less directly with ions traversing the pore . -
pubmed
Later on , after World War
II and the abolition of the
camphor monopoly , the company started manufacturing products related to oils and fats , including higher
fatty acids , and expanded its business by developing a new field of chemical industry . -
pubmed
Characterization of in vivo
keratin 19 phosphorylation on
tyrosine-391 . -
pubmed
Although
K19 is known to be phosphorylated on
tyrosine residue(s ) , conclusive site - specific characterization of these residue(s ) and identification potential
kinases that may be involved has not been reported . -
pubmed
METHODOLOGY / PRINCIPAL FINDINGS : In this study , biochemical , molecular and immunological approaches were undertaken in order to identify and characterize
K19 tyrosine phosphorylation . -
pubmed
Upon treatment with
pervanadate , a
tyrosine phosphatase inhibitor ,
human K19 (
hK19 ) was phosphorylated on
tyrosine 391 , located in the ' tail ' domain of the protein . -
pubmed
This
tyrosine residue is not phosphorylated under basal conditions , but becomes phosphorylated in the presence of
Src kinase in vitro and in cells expressing constitutively - active
Src . -
pubmed
An initial screen of osmolytes (
glycerol ,
sucrose ,
polyethylene glycol ,
2,4 - methylpentanediol ,
glutamic acid ,
arginine ,
glycine ,
proline ,
betaine ,
sarcosine , and
trimethylamine N - oxide ) reveals that
glycine , along with similarly structured compounds ,
betaine and
sarcosine , protects
AP - aldolase structure and activity from thermal inactivation . -
pubmed
Consistent with these results , epigenetic analysis , such as pyrosequencing ,
bisulfate sequencing and
methyl - specific
polymerase chain reaction (
MSP ) , showed that the expression level of
ZNF312b is highly dependent on the degree of DNA methylation in
gastric cancer cell lines . -
pubmed
In addition , by ChIP assay using anti -
acetyl /
methyl H3K9 antibodies , histone acetylation was shown to mediate the expression of the
ZNF312b gene . -
pubmed
Phosphorescence quenching was used to quantify Pm(
O ( 2 ) ) in vivo at
rest and across the
rest - to - twitch contraction ( 1 Hz , 5 min ) transition in the spinotrapezius muscle . -
pubmed
Development and validation of a RP - HPLC method for stability - indicating assay of
gemifloxacin mesylate including identification of related substances by LC - ESI -
MS /
MS , 1H and 13C NMR spectroscopy . -
pubmed
A validated stability indicating RP - HPLC assay of
gemifloxacin mesylate was developed by separating its related substances on an Inertsil - ODS3V -
C18 ( 4.6 x 250 mm ; 5 mum ) column using 0.1 %
trifluoroaceticacid ( pH 2.5 ) and
methanol as a mobile phase in a gradient elution mode at a flow rate of 1.0 mL / min at 27 C. The column effluents were monitored by a photodiode array detector set at 287 nm . -
pubmed
Forced degradation of
gemifloxacin (
GFX ) was carried out under acidic , basic , thermal , photolysis and
peroxide conditions and the degradation products were separated and characterized by ESI -
MS /
MS ,
( 1 ) H and
( 13 ) C NMR spectroscopy . -
pubmed
Aliphatic carboxylates were generally millimolar
hCA I and II inhibitors and low micromolar / submicromolar
beta - CA inhibitors . -
pubmed
ITPases are
nucleoside triphosphate pyrophosphatases that clean the precursor pools of the non - canonical
triphosphates of
inosine and
xanthine . -
pubmed
ITPA is clinically important because a widespread polymorphism , 94C > A , leads to null
ITPase activity in erythrocytes and is associated with an adverse reaction to
thiopurine drugs . -
pubmed
We further show that
ITPA knockdown results in elevated mutagenesis in response to
HAP treatment . -
pubmed
This implies that individuals with defective
ITPase are predisposed to genome damage by impurities in
nucleotide pools , which is drastically augmented by therapy with
purine analogs . -
pubmed
Our results suggest that the
hMGL cysteine mutant maintains the same overall architecture as wild - type
hMGL . -
pubmed
The results also underscore the superior nucleophilic nature of the reactive catalytic
Ser(122 ) residue as compared to that of
Cys(122 ) in the sol - S - hMGL mutant and suggest that the nucleophilic character of the
Cys(122 ) residue is not commensurately enhanced within the three dimensional architecture of
hMGL . -
pubmed
LC /
MS analysis of tryptic digests from sol - S -
hMGL directly demonstrate covalent modification of this variant by
NAM and
AM6580 , consistent with enzyme
thiol alkylation and carbamoylation , respectively . -
pubmed
These data provide insight into
hMGL catalysis , the key role of the nucleophilic character of
Ser(122 ) , and the mechanisms underlying
hMGL inhibition by different classes of small molecules . -
pubmed
Z - VAD - FMK , an inhibitor of classical apoptosis , was unable to reverse the suppressive effect of
anti - GPC . -
pubmed
The results indicate that
PPV does not induce
type I interferon and that the
NS2 protein of PPV could blocks dsRNA - induced
IFN - beta promoter activation . -
pubmed
The L / F -
SD and L / F -
WS supplemented diets had equal proportion of 500 ppm
laminarin and fucoidan . -
pubmed
The Y665F and N642H mutant constructs increased the transcriptional activity of
STAT5 and
tyrosine ( Y694 ) phosphorylation , which was also observed in patient samples . -
pubmed
Analysis of molecular species composition revealed that around 50 % of 36 :x ( number of
carbons in the
acyl chains : number of double bonds )
MGDG was hydrolysed and
diacylglycerol (
DAG ) used for phospholipid synthesis , while another
MGDG fraction was converted into
digalactosyldiacylglycerol via the
DGD1 / DGD2 pathway and subsequently into oligogalactolipids by
SFR2 . -
pubmed
The accumulation of
oligogalactolipids and
PI in the
chloroplast and extraplastidial membranes , respectively , increases the concentration of
hydroxyl groups and enhances the ratio of bilayer- to non - bilayer - forming lipids , thus contributing to protein and membrane stabilization . -
pubmed
METHODS : Hazard ratios and 95 % confidence intervals ( 95 % CI ) from Cox proportional hazards analyses of mortality versus metabolic equivalent hours per day of exercise (
MET - hours per day , where 1
MET = 3.5 mL
O2 kg min , or approximately 1 - km run ) . -
pubmed
Common polymorphism ( 81Val>Ile ) and rare mutations ( 257Arg>
Ser and 335Ile>
Ser ) of the
MC3R gene in obese Polish children and adolescents . -
pubmed
NSAIDs are effective anti - inflammatory and analgesic drugs by virtue of their ability to inhibit biosynthesis of
prostaglandins at the level of the
cyclooxygenase enzyme . -
pubmed
Development of novel
N - linked aminopiperidine - based mycobacterial
DNA gyrase B inhibitors : scaffold hopping from known antibacterial leads . -
pubmed
Omarigliptin (
MK-3102 ): a novel long - acting
DPP-4 inhibitor for once - weekly treatment of
type 2 diabetes . -
pubmed
AIP1 is a major
cofactor of the
actin depolymerizing factor /
cofilin in eukaryotes , promoting
actin depolymerizing factor / cofilin - mediated actin disassembly . -
pubmed
This protein was purified after three chromatographic steps on cation exchange CM - Sepharose fast flow , size - exclusion column Sephacryl S-300 and anion exchange Capto Q. Bothropoidin was homogeneous by
SDS - PAGE under reducing and non - reducing conditions , and comprised a single chain of 49,558 Da according to MALDI
TOF analysis . -
pubmed
Bothropoidin induced
haemorrhage [ minimum
haemorrhagic dose ( MHD ) = 0.75 microg ] , inhibited
platelet aggregation induced by
collagen and
ADP , and interfered with viability and cell adhesion when incubated with endothelial cells in a dose and time - dependent manner . -
pubmed
Mn - superoxide dismutase ) , ( 3 ) the production of reactive
oxygen and
nitrogen species under stress conditions was markedly decreased , with the significant alleviation of concomitant protein oxidation . -
pubmed
CONCLUSION : With few exceptions like
ALDH2 * 2 , the contribution of individual genetic variants to the risk for
alcohol - related disorders is small . -
pubmed
PFs for
metiram and
pyraclostrobin related to washing and oil dipping were 0.47 and 0.41 , and 0.78 and 0.63 at single dose ( SD ) and double dose (
DD ) , respectively . -
pubmed
aureus is influenced by acquisition of the
methicillin resistance gene
mecA. -
pubmed
Recent findings indicate that
endothelial NOS and reactive
oxygen species ( ROS ) are significantly increased during hypoxic stress and are modulated by antioxidants . -
pubmed
However , the influence of antioxidants and
steroids on
nNOS upregulation by
hypoxia is largely unknown . -
pubmed
Hypoxia exposure ( dissolved
oxygen , DO : 1.7 mg / L for 2 and/or 4weeks ) caused significant increases in hypothalamic
nNOS mRNA , protein and its neuronal expression . -
pubmed
In contrast , treatments with
Nomega - nitro - L - arginine methyl ester , a competitive
NOS - inhibitor , or
vitamin E , an antioxidant , prevented the upregulation of
O2 ( - ) production and
nNOS expression in
hypoxia - exposed ( DO : 1.7 mg / L for 4 weeks ) fish . -
pubmed
Taken together , the results suggest that upregulation of
nNOS and ROS in the croaker hypothalamus in response to
hypoxia is influenced by antioxidant and overall
estrogen status . -
pubmed
Carbonic Anhydrase Activity Monitored In Vivo by Hyperpolarized
13C - Magnetic Resonance Spectroscopy Demonstrates Its Importance for pH Regulation in
Tumors . -
pubmed
Carbonic anhydrase buffers tissue pH by catalyzing the rapid interconversion of
carbon dioxide (
CO2 ) and
bicarbonate (
HCO3 ( - ) ) . -
pubmed
We assessed the functional activity of
CAIX in two colorectal
tumor models , expressing different levels of the enzyme , by measuring the rate of exchange of hyperpolarized
( 13 ) C label between
bicarbonate (
H ( 13 ) CO3 (- ) ) and
carbon dioxide (
( 13 ) CO2 ) , following injection of hyperpolarized
H ( 13 ) CO3 (- ) , using
( 13 ) C - magnetic resonance spectroscopy (
( 13 ) C -
MRS ) magnetization transfer measurements . -
pubmed
( 31 ) P -
MRS measurements of the chemical shift of the pH probe ,
3 - aminopropylphosphonate , and
( 13 ) C -
MRS measurements of the
H ( 13 ) CO3 (-)/( 13 ) CO2 peak intensity ratio showed that
CAIX overexpression lowered extracellular pH in these
tumors . -
pubmed
Specifically , we review how even single
amino acid changes in T cell epitopes can lead to loss of binding to
major histocompatibility complex (
MHC ) , lack of cross - reactivity , or antagonism and immune interference , where simultaneous or sequential stimulation with related variants of the same T cell epitope can cause T cell anergy or the conversion of effector to immunosuppressive T cell phenotypes . -
pubmed
Preparation and Biological Activity of New
Collagen Composites , Part I : Collagen /
Zinc Titanate Nanocomposites . -
pubmed
The aim of this investigation was to develop new antimicrobial
collagen /
zinc titanate (
ZnTiO3 ) biomaterials using a sol - gel cryogenic draying technology in keeping the native
collagen activity . -
pubmed
Three mechanisms of the antimicrobial action were supposed , including ( i ) mechanical demolition of the cell wall and membrane by the crystal nanoparticles of the
ZnTiO3 entrapped in the
collagen matrix , ( ii ) chelation of its metal ions , and ( iii ) formation of free
oxygen radicals due to the interaction between the microbial cells and antimicrobial agent . -
pubmed
Moreover , PM2.5 increased the levels of intracellular reactive
oxygen species ( ROS ) and the nuclear translocation of
NF - E2 - related factor-2 (
Nrf2 ) . -
pubmed
This pathway is comprised of
glucose phosphorylation via
polyphosphate glucokinase ,
NADH generation catalyzed by
glucose 6 - phosphate dehydrogenase (
G6PDH ) and
6 - phosphogluconate dehydrogenase (
6PGDH ) , electron transfer from
NADH to the anode , and
glucose 6 - phosphate regeneration via the non - oxidative
pentose phosphate pathway and gluconeogenesis . -
pubmed
In
breast carcinoma cells , high levels of hyaluronan ( HA ) and its
CD44 receptor are frequently associated with alteration in
estrogen signaling . -
pubmed
Albeit without effect on
ERalpha expression and
estradiol binding properties ,
HAS2 overexpression increased
ERalpha Ser118 phosphorylation as well as transcriptional activity of
estrogen in an ERE -
luciferase reporter gene assay . -
pubmed
However ,
HAS2 overexpression induced partial silencing of
E2 driven - genes without affecting the magnitude of regulation by
estradiol . -
pubmed
This effect was associated with half - reduction in the activity of nuclear
histone deacetylases (
HDACs ) through a post - translational mechanism likely consecutive to the enhanced expression of the histone
acetyl - transferase
EP300 . -
pubmed
In conclusion , increase in
HA /
CD44 interactions may contribute , through an
HDAC inhibitor - like and
ER - independent mechanism , to the silencing of
estrogen - driven genes in
breast carcinoma . -
pubmed
Methods
rFVIIIFc and its isolated
FVIII and Fc components were compared by the use of
hydrogen -
deuterium exchange mass spectrometry (
HDX -
MS ) . -
pubmed
Cytokine expression levels in
ALS : A potential link between
inflammation and
BMAA - triggered protein misfolding . -
pubmed
Furthermore , we propose a mechanism by which a known neurotoxin ,
beta - N - methylamino - l - alanine (
BMAA ) , may trigger this
cytokine expression profile through motor neuron protein misfolding and subsequent
NLRP3 (
nucleotide - binding domain ( NOD )- like receptor protein 3 ) inflammasome activation . -
pubmed
Inhibition of
STAT3 signaling pathway by
ursolic acid suppresses growth of
hepatocellular carcinoma . -
pubmed
The effects of miRNA on the intracellular concentration and
cytotoxicity of
5 - FU in
CRC cells were measured by high performance liquid chromatography conjected tandem mass spectrometry ( HPLC -
MS /
MS ) and
MTT methods , respectively . -
pubmed
Statistical analysis showed that a polymorphism rs3742106 in the 3'-UTR of
ATP - binding cassette subfamily C member 4 (
ABCC4 ) gene was significantly associated with the efficacy of
5 - FU /
capecitabine - based chemotherapy in
CRC . -
pubmed
Opioid agonist treatment (
OAT ) with
methadone or
buprenorphine has strong evidence for treatment of this relapsing condition . -
pubmed
In a recent randomized controlled trial , we demonstrated high rates of successful rapid referral from a syringe exchange programme (
SEP ) to treatment with
methadone or
buprenorphine , including actual treatment initiation . -
pubmed
Median time to
PSA progression was not reached with
abiraterone versus 3.8 months for
prednisone , attaining 58 % reduction in
PSA progression risk ( HR = 0.418 ; p & lt ; 0.0001 ) . -
pubmed
In 2013 , before switching to generic
imatinib , all patients had reached
MMR in accordance with European LeukemiaNet (
ELN ) Guidelines . -
pubmed
Two different dry
TOFA formulations (
silica carrier and
palm oil coating ) were tested and showed performance effects similar to liquid
TOFA . -
pubmed
Searching for correlation between DNAmeth of the studied genes with demographic characteristics and baseline biochemical parameters of the studied population , we observed a correlation between
IGF2 methylation and
folate ( r = 0.44 , p = 0.003 ) . -
pubmed
Joint associations of serum
uric acid and
ALT with
NAFLD in elderly men and women : a Chinese cross - sectional study . -
pubmed
BACKGROUND : Epidemiological evidence suggests sex difference in serum
uric acid ( SUA ) and
alanine aminotransferase (
ALT ) might be a potential explanation for the gender difference in prevalence of
non - alcoholic fatty liver disease (
NAFLD ) . -
pubmed
, gas production , pH , and short
carboxylic acid concentrations , were compared to an untreated control at 96 h
post inoculation . -
pubmed
Seed mucilage RG - I with short side chains and unusual
O3 substitution showed loose
coils or
star - like conformations . -
pubmed
All four chemicals interfered with
estradiol - mediated induction of
PGR ,
NPPC , and
GREB1 . -
pubmed
The difficulty of accessing reference S - phosphorylated peptides ( pCys - peptides ) hampers progress in
MS - driven
cysteine phosphoproteomics , which requires targeted analytical procedures . -
pubmed
Hen egg - white
lysozyme was reduced with
tris ( 2 - carboxyethyl ) phosphine (
TCEP ) followed by alkylation of
cysteine with
( 3 - acrylamidopropyl ) trimethyl - ammonium chloride (
APTA ) and subsequent beta - elimination in aqueous
Ba ( OH ) 2 to yield modified polypeptides containing multiple
dehydroalanine (
Dha ) residues . -
pubmed
The conjugate addition of
thiophosphoric acid to
Dha residues followed by trypsinolysis led to numerous D / L
phosphocysteine - containing peptides , which were identified by higher - energy collisional - dissociation tandem mass spectrometry (
HCD -
MS /
MS ) . -
pubmed
miR microarray analysis and PCR validation from CD4 + T cells showed treatment with
resveratrol decreased the expression of several miRs ( miR-31 , Let7a , miR-132 ) that targeted
cytokines and
transcription factors involved in anti - inflammatory T cell responses (
Foxp3 and
TGF - beta ) . -
pubmed
Although the mutation analysis performed on DNA isolated from blood cells and progressed lesion samples confirmed the
tumor to be germline
BRCA wild - type ,
cisplatin monotherapy was administered based on the increasing evidence of safety and efficacy of
platinum for
breast cancer . -
pubmed
In addition ,
vimentin expression was unaltered in comparison with the control , results that indicated an improvement in the
CoCr biocompatibility with the
ErGO on the material surface . -
pubmed
Up to five size fractions of
microcrystalline cellulose (
MCC ) ,
dibasic calcium phosphate (
DCP ) , and
lactose monohydrate (
lactose ) in the range of < 125 microm up to the range of 355 - 500 microm were compressed into tablets and analysed with THz - TDS . -
pubmed
This observation was more pronounced for
DCP , followed by
lactose and then
MCC and the effect was more pronounced for larger compared to smaller initial particles . -
pubmed
Suspending
P2Y12 inhibitors while
aspirin continued before elective CABG is recommended , as well as 12 months of DAPT following CABG . -
pubmed
It is evident that the
glutaraldehyde test gives a good idea of the
immunoglobulin status of fatteners . -
pubmed
Melatonin : deacetylation to
5 - methoxytryptamine by liver but not brain
aryl acylamidase . -
pubmed
Increases in
pseudouridine excretion suggest increased
tumor transfer ribonucleic acid turnover ; in addition , for the
methylated nucleosides , higher than normal values may reflect enhanced
transfer ribonucleic acid methylase activity of the neoplastic cells . -
pubmed
Use of the
TNF - alpha blocker ,
pentoxifylline , has also shown promise in lessening
VOD . -
pubmed
Some of the laboratory factors that did not show significant association with treatment response were the initial
creatinine ,
hemoglobin , platelet count ,
lactate dehydrogenase , and total
bilirubin . -
pubmed
Synthesis and
benzodiazepine receptor binding of
1H - pyrrolo [ 2,1 - C ][ 1,4 ] benzothiazines . -
pubmed
Cyclosporin A inhibits the
interleukin 2 receptor alpha chain gene transcription but not its cell surface expression : the
alpha chain stability can explain this discrepancy . -
pubmed
We have previously shown that
CsA prevents the constitutive transcription of the
IL2 gene in these cells . -
pubmed
Here we show that as soon as 4 h after
CsA addition , the transcription of the gene encoding the
alpha chain ( p55 ) of
IL2R was inhibited . -
pubmed
CsA did not prevent this transient
IL2 - dependent induction of
IL2R alpha , but could still partially inhibit it . -
pubmed
Once
IL2 induction was over ,
CsA exerted its full inhibition . -
pubmed
Thus ,
CsA does not seem to inhibit IL 2R alpha gene transcription simply by inhibition of
IL2 synthesis . -
pubmed
However , no modification of
IL2R alpha expression on the cell surface could be detected after 48 h in the presence of
CsA. -
pubmed
This discrepancy between the effect of
CsA on
IL2R alpha expression as probed at the mRNA or the protein level can be accounted for by the stability of the
IL2R alpha protein after synthesis . -
pubmed
These results strongly suggest that the truncated
gramicidins form a novel conducting channel which differs from the
gramicidin head - to - head , single - stranded beta
6.3 - helical dimer ( " channel " ) in its conductance characteristic and its structure. -
pubmed
When cells were lysed in the presence of 0.05 - 10 microM
CaCl2 , the soluble
5 - lipoxygenase became associated with the particulate fraction . -
pubmed
Ca2 + induced the membrane association of
5 - lipoxygenase when added into the incubation mixtures containing the membrane fraction with either the cytosolic fraction or the purified
enzyme . -
pubmed
5 - Lipoxygenase also bound to the microsomal - enriched fraction in the presence of
Ca2 + . -
pubmed
5 - Lipoxygenase binding to the membrane fraction was unaffected by pretreatment of the membranes with
trypsin but was inhibited by treating with
phospholipase A2 , suggesting that
phospholipids are involved. -
pubmed
, the
pigmented area of the distal nerve ) the punctate
laminin matrix is missing . -
pubmed
By contrast , in the presence of
l - PA or
dl - PA new
actinomycin components containing
PA were synthesized at the expense of normally produced components . -
pubmed
Although
PA - containing
actinomycins were formed as early as 6 h after the addition of 250 mug of
l - PA per ml , it was not until 24 h postaddition that the relative percentages of
actinomycin components produced approached a constant value . -
pubmed
Ferredoxin - dependent
phenylpyruvate synthesis by cell - free preparations of photosynthetic bacteria . -
pubmed
Because of its extensive binding ( greater than 99 % ) to the
protein fraction of the perfusate , the urinary excretion of
carprofen was low an perfusate concentration - dependent . -
pubmed
In contrast , the receptor - mediated uptake and degradation of
mannosyl bovine serum albumin was increased three - fold . -
pubmed
The effect of the
Mo ( t ) supernatant was specific for macrophages and was abolished by preincubation of the supernatant with
trypsin , which indicates that the active substances are protein in nature . -
pubmed
The decrease in the uptake and degradation of
MDA - LDL induced by preincubating the macrophages with
Mo ( t ) supernatant appeared to result from a decrease in the number of receptors for this ligand at the cell surface . -
pubmed
Esterase - like activity of
human serum albumin : enantioselectivity in the burst phase of reaction with
p - nitrophenyl alpha - methoxyphenyl acetate . -
pubmed
CXD showed higher concentration than
CEX ,
CED and
CFT . -
pubmed
The average recovery rates within 6 hours were 93.6 , 88.3 and 94.3 % respectively and they were similar to those of
CEX ,
CED and
CFT . -
pubmed
Elimination of inhibitors of
alkaline phosphatase from
2 - amino-2 - methyl-1 - propanol . -
pubmed
When
2 - amino-2 - methyl-1 - propanol buffer solutions are extracted with
chloroform , impurities inhibiting
alkaline phosphatase are selectively removed and this source of variation is thereby greatly decreased . -
pubmed
Augmentation of
glucose transport into skeletal muscle by
GLUT4 translocation to the plasma and T - tubule membranes can be mediated independently by
insulin and by contraction / exercise . -
pubmed
Fractions of 32 and 36 %
sucrose exhibited the highest enrichment of
GLUT4 and were independently responsive to
insulin and exercise , respectively . -
pubmed
The contraction- and
insulin - sensitive
GLUT4 pools also contained secretory component - associated membrane protein /
glucose transporter vesicle triplet but not
GLUT1 and
caveolin . -
pubmed
On the other hand , sedimentational analysis in
sucrose velocity gradients revealed that the
insulin - sensitive
GLUT4 vesicles had a larger sedimentation coefficient than the exercise - sensitive vesicles . -
pubmed
The fact that both children exhibited the rare Bombay blood group and were
Lewis negative , each involving
carbohydrates with different
fucose linkages , suggested a possible defect in the
fucose - containing ligand for
E - and P - selectin , sialyl Lewis X (
SLe ( x ) ) . -
pubmed
Adhesion of neutrophils to endothelial cells activated with
interleukin-1 beta or
histamine was markedly decreased ( & lt ; 5 % of control ) . -
pubmed
Tubulin polymerization by
paclitaxel (
taxol )
phosphate prodrugs after metabolic activation with
alkaline phosphatase . -
pubmed
Paclitaxel (
taxol )
phosphate derivatives
BMY46366 ,
BMY-46489 ,
BMS180661 and
BMS180820 were used to determine the ability of
alkaline phosphatase to convert these water - soluble potential prodrugs to
tubulin - polymerizing metabolites ( i.e. -
pubmed
Compounds were treated up to 180 min with an in vitro metabolic activation system composed of 10 %
bovine alkaline phosphatase in 0.2 M
tris , pH 7.4 , or in 0.2 M
glycine , pH 8.8 , plus 0.05 M
MgCl2 . -
pubmed
Pretreatment of
2 '- and 7 - phosphonoxyphenylpropionate prodrugs
BMS180661 and
BMS180820 with
alkaline phosphatase for 30 to 120 min yielded relative initial slopes of about 20 to 100 % at test concentrations equimolar to
paclitaxel . -
pubmed
High - performance liquid chromatography / mass spectrometry of BMS180661 treated with
alkaline phosphatase confirmed the production of
paclitaxel from the prodrug . -
pubmed
In contrast ,
2 '- and 7 - phosphate analogs
BMY46366 and
BMY46489 treated with
alkaline phosphatase were not active in
tubulin assays . -
pubmed
None of the
paclitaxel phosphate prodrugs polymerized
tubulin in the absence of metabolic activation . -
pubmed
The differences in
tubulin polymerization with metabolic activation may be related both to accessibility of the
phosphate group to the enzyme and to anionic charge effects . -
pubmed
These results demonstrate that certain
paclitaxel phosphate prodrugs can be metabolized by
alkaline phosphatase to yield effective
tubulin polymerization . -
pubmed
In fact ,
cAMP acted in two ways to stop IPC promyelocyte proliferation : ( 1 ) block in late
G1 ( preventing new cells from entering DNA replication ) ; and ( 2 ) induction of apoptosis in S - phase . -
pubmed
TEM experiments conducted on SLPF droplets cast from
formic acid on amorphous
carbon film demonstrated that these protein films have a microstructure formed of woven sheaves . -
pubmed
TCR beta rearrangements involving gene segments from the
V beta 1 gene family can be detected beginning on day 12 of development , while rearrangements involving the other family of V beta gene segments , V
beta 2 , were first detected on day 14 of embryogenesis . -
pubmed
VIP and
preproVIP immunoreactivity was present in
somata and nerve fibres of all central ganglia . -
pubmed
The mean plasma concentration of
human chorionic gonadotrophin and
progesterone for the
ectopic pregnancy group was significantly lower than that for the singleton delivery group ( P & lt ; 0.001 ) . -
pubmed
This information was used to interpret the full
oligosaccharide profiles of
glycoproteins in a predictive manner based on arm specificity , linkage , and
monosaccharide composition . -
pubmed
Second , a serum
IgG glycan pool was resolved into 20 peaks which were analyzed simultaneously by sequentially digesting the pool of
sugars with
exoglycosidase enzymes . -
pubmed
The reproducibility of the separation system , the predictability of
glucose unit values , and the quantitative response of the detection system for individual fluorescently labeled glycans also allowed the automatic analysis of neutral
sugars using combinations of
enzymes as in the reagent array analysis method ( RAAM ) . -
pubmed
During the evolution of the autoimmune response to
myelin basic protein (
MBP ) , at least two distinct lymphocyte populations arise , one that is specific for determinants within the protein
antigen and another that is specific for the receptors displayed by the
antigen - specific lymphocytes themselves . -
pubmed
[
Apolipoprotein E and response to
statins ] . -
pubmed
The experimental strategy was to bypass upstream deficiencies by inducing normal , fertilization - like [
Ca2 +]i oscillations in fully grown , germinal vesicle ( GV ) stage oocytes and determine if the extent of CG exocytosis was restored to levels observed in mature , metaphase
II ( MII)-stage eggs . -
pubmed
These genes also showed differential responses in time course : more rapid induction was observed in the order of c - fos ,
hsp70 , and
MT - IIA after exposure to
Zn or
Cd . -
pubmed
By contrast ,
HSE - binding activity was detected in extracts from cells treated with
Zn ,
Cd ,
Ag , and
Cu . -
pubmed
Agrin is a major
heparan sulfate proteoglycan accumulating in
Alzheimer ' s disease brain . -
pubmed
However , while spread of
GFP -
VP22 was observed by examining intrinsic GFP fluorescence after
methanol fixation , little spread was observed after PFA fixation , suggesting that the levels of the fusion protein in recipient cells were below the detection limits of intrinsic - fluorescence or that
PFA fixation quenches the fluorescence of
GFP -
VP22 . -
pubmed
We further considered whether elution of
VP22 from
methanol - fixed cells and postfixation binding to surrounding cells contributed to the increased detection of spread observed after
methanol fixation . -
pubmed
The extracted samples were analyzed on a jordi - gel
DVB polyamine column ( operated under an
acetonitrile / water gradient with water as the stronger mobile phase ) interfaced with a Finnigan TSQ 7,000 mass spectrometer . -
pubmed
Allantoin and its internal standard (
13C1 , 15N1 - allantoin ) were detected by use of single reaction monitoring (
SRM ) mode . -
pubmed
Pharmacodynamic parameter estimates for
postoperative vomiting were
P0 ( the baseline probability of
vomiting , with no opioid ) 0.115 , Pmax ( the maximal probability of
vomiting due to
morphine ) 0.997 , ED50 (
morphine dose that induces an effect equivalent to 50 % of the logit Pmax ) 0.18 mg / kg . -
pubmed
Parameter estimates for overnight stay because of
vomiting after
morphine administration were
P0 0.038 , Pmax 0.999 , ED50 0.369 mg / kg . -
pubmed
Soluble
N - ethyl maleimide - sensitive fusion protein attachment protein receptors (
SNAREs ) are core machinery for membrane fusion during intracellular vesicular transport . -
pubmed
Comparing the
PI arms at week 48 showed significantly higher levels of PT in the
ritonavir - containing arms than in the
indinavir arm ( p & lt ; 0.001 ) . -
pubmed
In Experiment 1 , the effects of varying concentrations of
BCAA and
ARG in the TPN infusate , singly and together , were assessed : Group 1 , Standard
TPN ( 19 %
BCAA , 4.8 g
ARG / L ) ; Group
II , High
BCAA TPN ( 35 %
BCAA , 4.8 g
ARG / L ) ; Group III , High
ARG TPN ( 19 %
BCAA , 9.6 g
ARG / L ) ; Group
IV , High
ARG , High
BCAA TPN ( 35 %
BCAA , 9.6 g
ARG / L ; Group V , chow and tap water ad libitum . -
pubmed
Serum total
bilirubin was significantly higher in Group I than in normals and in Groups
II , III , and V. Serum
lactate dehydrogenase levels were similar in normals and all 5 groups . -
pubmed
The biochemical and physiological adaptations of plants to low temperatures include the
post - translational activation and increased expression of enzymes of the
sucrose synthesis pathway , the changed expression of Calvin cycle enzymes , and changes in the leaf protein content . -
pubmed
Then , data from 8779 women screened using the quadruple test (
alphafetoprotein (
AFP ) , unconjugated
estriol ( uE(3 ) ) ,
human chorionic gonadotrophin ( free
beta - hCG ) , and
inhibin - A levels with maternal age ) were used to determine the effect of smoking on the serum markers . -
pubmed
Apomorphine , the
catechol - derived
dopamine D1 / D2 receptor agonist , is currently in use as an antiparkinsonian drug . -
pubmed
The action of
apomorphine can be mimicked by
dopamine receptor (
D1 and
D2 ) agonists or blocked by preincubation with
D1 / D2 receptor antagonists . -
pubmed
Increased mRNA expression levels of
brain - derived neurotrophic factor and
glial cell line - derived neurotrophic factor were also found in the
apomorphine - treated mesencephalic cells along with concomitant protein expression increases in the conditioned medium . -
pubmed
These results suggest that the
apomorphine - modulated development of dopaminergic neurons may be mediated by activation of the
dopamine receptor subtypes
D1 and
D2 thereby increasing the production of multiple
growth factors . -
pubmed
Cell viability and
cytotoxicity were assessed by
dimethylthiazolyldiphenyltetrazolium bromide (
MTT ) testing and quantification of
lactate dehydrogenase . -
pubmed
RESULTS : Neither
keratin synthesis as a late marker of cell differentiation nor mitochondrial cell activity were influenced by either
zinc compound . -
pubmed
The synthesis of
involucrin , an early marker of differentiation , was significantly increased by both
zinc salts ,
ZnSO ( 4 ) being the more potent stimulator . -
pubmed
Effect of reaction parameters on synthesis of
citronellyl methacrylate by
lipase - catalyzed transesterification . -
pubmed
The
methacrylate ester of
citronellol was synthesized using various
lipases as catalyst . -
pubmed
The effect of different reaction parameters such as amount of
lipase , solvent , temperature , and acylating agent on the conversion of
citronellol to
citronellyl methacrylate was studied . -
pubmed
One -
pot route to new
alpha , alpha - difluoroamides and
alpha - ketoamides . -
pubmed
New
alpha , alpha - difluoroamides ( 4a - d , 6a - d ) and
alpha - ketoamides ( 5a - d , 7a - d ) result from one -
pot reactions of
alpha - ketoacids ,
RCOCO ( 2 ) H ( R = C ( 6 ) H ( 5 ) ,
CH ( 3 ) ,
CH ( 3 ) CH ( 2 ) , thienyl ) ( 1a - d ) with
bis ( 2 - methoxyethyl ) aminosulfur trifluoride [
( CH ( 3 ) OCH ( 2 ) CH ( 2 ))( 2 ) NSF ( 3 ) ] ( 2 ) (
Deoxofluor ) or
diethylaminosulfur trifluoride [
( CH ( 3 ) CH ( 2 ))( 2 ) NSF ( 3 ) ) ] ( 3 ) (
DAST ) . -
pubmed
In the Danish cohort ( n = 306 ) of the Early Postmenopausal Intervention Cohort ( EPIC ) Study ( n = 1609 ) of oral
alendronate (
ALN ) for prevention of
postmenopausal osteoporosis , bone markers (
urine C - telopeptides of
type I collagen (
uCTX ) , urine N - telopeptide cross - links of
type I collagen (
uNTX ) , serum total
osteocalcin measured by ELISA [ total OC ( ELISA ) ] , and serum total
osteocalcin measured by RIA [ total OC ( RIA ) ] ) were measured at 6 - month intervals . -
pubmed
ALPHA - AMYLASES AS CALCIUM - METALLOENZYMES . -
pubmed
The drug
salicylamide is an antagonist of the
aryl hydrocarbon receptor that inhibits signal transduction induced by
2,3,7,8 - tetrachlorodibenzo - p - dioxin . -
pubmed
The detrimental effects of
TCDD are mediated via the
aryl hydrocarbon receptor (
AhR ) , a
transcription factor that regulates the expression of the carcinogen - activating enzymes
cytochromes P-450 ( CYP ) 1A1 , 1A2 , and 1B1 . -
pubmed
Salicylamide (
SAL ) , an analgesic drug , caused a potent and long - lasting inhibition of
TCDD - induced
CYP enzyme activity . -
pubmed
Acetylsalicylic acid (
aspirin ) and the naturally occurring phytochemical
salicylic acid had no effect on
CYP activity . -
pubmed
SAL inhibited the increase in
CYP1A1 , - 1A2 , and - 1B1 mRNA levels that occurs on exposure to
TCDD . -
pubmed
TCDD - induced transcription of these genes was also inhibited by
SAL , but not by
aspirin or
salicylic acid , as demonstrated by
luciferase reporter assays . -
pubmed
The transcription of the
CYP1 family of genes is regulated by the interaction of
TCDD - activated
AhR with the xenobiotic - responsive element present in the promoter regions of these genes . -
pubmed
As shown by electrophoretic mobility shift assay ,
SAL completely blocked the binding of
TCDD - activated
AhR to the xenobiotic responsive element . -
pubmed
Also ,
SAL substantially blocked the binding of
TCDD to the cytosolic
AhR. -
pubmed
These results demonstrate that
SAL , a commonly used analgesic , is a potent inhibitor of
AhR - mediated signal transduction , and may be an effective agent in the prevention of
TCDD - associated disease . -
pubmed
Activation of
STAT3 and
MAPK were manipulated by using dominant - negative
STAT3 recombinant adenoviruses and a specific
inhibitor of
MAPK , respectively . -
pubmed
RESULTS : Blocking of the
MAPK pathway by the
MAPK inhibitor
PD98059 did not affect normal development of rods in retina explants or the suppression of their appearance by treatment with
CNTF . -
pubmed
In an effort to understand the mechanism , we also solved and report the structures of
YbiV in complex with
beryllofluoride (
BeF3- ) and
aluminum trifluoride (
AlF3 ) , which have been shown to mimic the phosphorylated intermediate and transition state for hydrolysis , respectively , in analogy to other
HAD phosphatases . -
pubmed
Both structure and sequence homology indicate
YbiV may be a
sugar phosphatase , which is supported by biochemical assays that measured the release of free
phosphate on a number of
sugar - like substrates . -
pubmed
Bicuculline , a
GABA ( A ) receptor antagonist , was then injected ( 0.10 - 0.15 microl , 5 mM ) into the MRF through a stereotaxically placed glass micropipette . -
pubmed
On the other hand , an injection of 0.10 - 0.15 microl of 5 mM
muscimol , a
GABA ( A ) receptor agonist , into the NRPv decreased the frequency of
SLN - induced swallowing . -
pubmed
However , these
enzymes are activated by
calcium or
manganese , and as a result inhibited by chelators and by
ATP , properties typical of neutral
trehalases . -
pubmed
Under these conditions the
enzyme was neither activated by
calcium nor inhibited by
EDTA or
ATP . -
pubmed
The neutral activity was estimated in
MES buffer at pH 6.5 , after subtracting the activity resistant to
EDTA inhibition . -
pubmed
AIMS : The main objectives of this study were to determine : ( 1 ) the relative roles of heavy episodic drinking (
HED ) , drinking frequency and drinking volume in explaining
alcohol - related
aggression and ( 2 ) whether drinking context variables ( i.e. -
pubmed
usual drinking locations , typical drinking companions and extent of peer drinking ) confound or modify the relationship between
HED and
alcohol - related
aggression or whether they predict
alcohol - related
aggression independently . -
pubmed
Serum aminotransferase levels increased transiently 2 months after the cessation of immunosuppressive therapy (
prednisolone ,
azathioprine [
AZP ] , and
CyA ) . -
pubmed
Compared to soluble proteins ,
MHC classes I and II - restricted presentation of
PLGA - MS - encapsulated proteins and peptides by MoDC was markedly prolonged and proteins were presented 50 - fold more efficiently on
class I molecules . -
pubmed
The apo and
pyruvate - bound structures of
PchB reveal that the enzyme is a structural homologue of
chorismate mutases in the
AroQalpha class despite low sequence identity ( 20 % ) . -
pubmed
Determining the structure of
PchB is part of a larger effort to elucidate protein structures involved in
siderophore biosynthesis , as these enzymes are crucial for bacterial
iron uptake and virulence and have been identified as antimicrobial drug targets . -
pubmed
Ascorbic acid and its derivative induced a twofold increase in
type VII collagen synthesis , and markedly increased the secretion of
type VII collagen into the medium when compared with the control culture . -
pubmed
For a given local
Vo ( 2 ) , leg vascular conductance ( VC ) was five- to sixfold higher than arm VC , despite marked
hemoglobin deoxygenation in the subclavian vein . -
pubmed
A forbidden reflection at 7.792 keV near the
Dy L3 absorption edge is identified as a quadrupolar ordering peak , and the quadrupolar order and a monoclinic structural distortion develop concomitantly below 12.3 K as second - order - type phase transitions . -
pubmed
Hematoxylin - eosin staining and immunohistochemistry for
Endonuclease G ,
Parvalbumin and
Glial fibrillary acidic protein was performed after the ischemic insult . -
pubmed
The magnitude of cell proliferation , measured by
bromodeoxyuridine incorporation , was inversely proportional to the level of
connexin32 expression . -
pubmed
Positron - emission tomography - computed tomography ( PET /
CT ) is rapidly being integrated into the imaging pathways of several different
tumour types , most frequently using the
glucose analogue
2 - [ ( 18 ) F ]- fluoro-2 - deoxy - D - glucose (
FDG ) . -
pubmed
Integrated
FDG - PET /
CT combines functional and anatomical imaging to improve sensitivity and specificity of
tumour detection . -
pubmed
Abstract We tested the hypothesis of a cross - inhibition of
oxytocin ( OT ) release by endogenous opioid peptides co - released with
vasopressin (
VP ) . -
pubmed
The effects of the
opiate receptor antagonist
naloxone were tested on neurohypophyseal
VP release during
dehydration ,
ethanol administration and
sulphated cholecystokinin octapeptide (
CCK-8S ) application , assuming that the inhibition of pituitary OT release by endogenous opioids increases as neurohypophyseal
VP output increases . -
pubmed
Naloxone had no effect on
VP release . -
pubmed
Ethanol ( 10 % in saline ) administered intragastrically 50 min prior to
decapitation and 20 min before subcutaneous
naloxone ( 5 mg / kg ) resulted in the inhibition of
VP output . -
pubmed
The
ethanol treatment resulted in a rise in plasma
OT levels that was additional to the effect of
naloxone . -
pubmed
Peripheral
CCK-8S administration induces an abrupt and selective secretion of
OT . -
pubmed
Blocking the opioid inhibition of
OT release with
naloxone resulted in a significant rise of
OT compared to that with
CCK-8S alone . -
pubmed
No effect of
CCK-8S and/or
naloxone was found on
VP plasma levels . -
pubmed
The data indicate that opioid peptides co - released with
VP ( like
dynorphin ) may be responsible for cross - inhibition of
OT release during
dehydration . -
pubmed
We reported , to our knowledge for the first time , that
PS - G could promote neurite outgrowth of neurons cultured on the myelin substrates and inhibit the activation of
RhoA. -
pubmed
tumefaciens strain C58C1Rif containing plasmid pTDE4 harbouring
neomycin phosphotransferase (
npt II ,
kanamycin resistance ) and
beta - glucuronidase encoding genes . -
pubmed
The Southern blot analysis has shown that 78 %
kanamycin resistant plants contain gene encoding
beta - glucuronidase . -
pubmed
High magnetic field proton magnetic resonance spectroscopy (
( 1 ) H -
MRS ) provides a reliable measurement of
GABA in specific regions of the brain . -
pubmed
In the
ACC , the
GABA concentration was negatively correlated with the dose of the antipsychotics ( rs=-0.347 , p=0.035 ) . -
pubmed
BACKGROUND : Both pramlintide and
insulin pump waveforms separately provide improved
post - meal
glucose control . -
pubmed
METHODS : Using the CGMS Gold ( Medtronics , Northridge , CA ) we measured the
glucose response 0 - 4 h after the beginning of a meal and 15 min after the initiation of the
insulin bolus wave and
pramlintide ( 60 microg ) , bolus . -
pubmed
RESULTS : With ST and C waves
glucose fell approximately 40 mg / dL 0 - 2 h
post - meal and then returned to baseline by the fourth hour . -
pubmed
In comparison with other European countries , the use of
RAAS antagonists is similar , there are more beta blockers and
nitrates used , but there is less use of antithrombotic therapy ( especially anticoagulation ) and of statins . -
pubmed
Epitaxial growth of
graphitic carbon on C -
face SiC and
Sapphire by chemical vapor deposition ( CVD ) . -
pubmed
We have measured the spin - polarized electron momentum density distributions of
EuFe2 ( As0.73P0.27 ) 2 by magnetic Compton scattering (
MCS ) measurements . -
pubmed
The
MCS orbitalwise decomposition of the density distributions reveals that between 16 and 19 K , the spin - polarized
Fe-3d character is enhanced ( as the ferromagnetic character supersedes superconducting character ) , where the resistivity shows a maximum , reentrant SC - like peak , at 18 K. The spin polarization of the
Fe-3d orbital , enhanced by ferromagnetic
Eu ions , suppresses the SC around 18 K , while at other temperatures the system indeed exhibits SC where the
Fe-3d spin polarization is suppressed or collapses . -
pubmed
OBJECTIVE : to develop a rapid and reliable real - time PCR to detect polymorphisms of
angiotensinogen (
AGT ) , to compare the two methods of
MS - PCR ( Mutagenically Separated PCR ) and real - time PCR to determine three polymorphisms of the
angiotensinogen gene M235 T , the A ( -6 ) G and A ( -20 ) C. METHODS : the method of real - time PCR was developed on the PLC Roche LightCycler1 with
SYBR Green I. We used two sense primers and a primer nonsense . -
pubmed
omega - Transaminase (
omega - TA ) is an industrially important enzyme for production of chiral
amines . -
pubmed
This study aims to construct a mathematical model to simulate reductions in
low - density lipoprotein ( LDL )
cholesterol in persons who combine the use of statins with a high intake of
phytosterols/-
stanols , e.g. -
pubmed
METHODS AND RESULTS : The proposed model includes the
cholesterol pool size in the liver and serum levels of
very low - density lipoprotein (
VLDL )
cholesterol . -
pubmed
Both an additional and a multiplicative effect of
phytosterol/-
stanol intake on
LDL cholesterol reduction were predicted from the model . -
pubmed
From the model , it was demonstrated that a daily intake of 2 g
phytosterols/-
stanols reduces
LDL cholesterol level by about 8 % to 9 % on top of the reduction resulting from statin use . -
pubmed
The additional decrease in
LDL cholesterol caused by
phytosterol/-
stanol use at the recommended level of 2 g / d appeared to be similar or even greater than the decrease achieved by doubling the statin dose . -
pubmed
Whereas in the
NP breast , nuclei of epithelial cells were large and euchromatic , in the P breast they were small and hyperchromatic , showing strong methylation of
histone 3 at
lysine 9 and 27 . -
pubmed
Downregulation of
BCL2 by
AT-101 enhances the antileukaemic effect of
lenalidomide both by an immune dependant and independent manner . -
pubmed
Lenalidomide , an immunomodulator , is clinically effective in
CLL and can enhance the anti - CLL effects of
CD20 targeting monoclonal antibody ,
rituximab . -
pubmed
Here , we investigated the mechanism of immune - directed killing of
lenalidomide in
CLL and evaluated if concurrent targeting of
CD20 and
BCL2 can enhance this effect . -
pubmed
This effect was further enhanced with pre - treatment of
tumour cells with
AT-101 ( a BH3 mimetic that functions as
BCL2 antagonist ) . -
pubmed
Neurogenesis was assessed by analysis of cells expressing
NeuN , a neuronal marker , and
5 - bromo-2 '- deoxyuridine (
BrdU ) uptake . -
pubmed
Immunohistochemical analysis showed that the number of
BrdU /
NeuN double - labeled cells in the dentate gyrus was significantly decreased 30 days after
OBX . -
pubmed
Chronic treatment with Comp.1 , Comp.2 or
fluoxetine significantly increased the number of
BrdU /
NeuN double - labeled cells . -
pubmed
Morphine - induced signaling via
opioid receptors (
ORs ) in dorsal root ganglia ( DRG ) neurons , the spinal cord , and various brain regions has been shown to modulate gene activity . -
pubmed
Overall , we reveal an important role of
ELK-1 for
morphine - dependent gene induction in DRG - derived cells and propose that
ELK-1 and
EGR-4 contribute to the effects of
morphine on neuronal plasticity . -
pubmed
RESULTS :
mCPP significantly attenuated
quinpirole - induced
compulsive checking behavior by reducing vigor of checking ( indexed by frequency of checking and length of check ) and increasing
rest after a bout of checking ( indexed by time to the next checking bout ) , but it did not affect focus on the task of checking ( indexed by
recurrence time of checking and number of stops before returning to check ) . -
pubmed
Ameliorative effects of
mCPP may involve
5HT2A / 2C receptors in substantia nigra pars reticulata that inhibit expression of motor vigor . -
pubmed
Moreover ,
integrin beta1 , through its abilities to activate latent
TGFbeta1 and promote
collagen production through
focal adhesion kinase / rac1 / nicotinamide adenine dinucleotide phosphate oxidase (
NOX)/reactive
oxygen species ( ROS ) , is essential for dermal homeostasis , repair and
fibrosis . -
pubmed
The gas - phase
oxygen -
hydrogen exchange reaction dynamics of
O (( 3 ) P ) + i - C3H7 ( isopropyl ) H (( 2 ) S ) +
CH3OCH3 (
acetone ) was first investigated by the vacuum - ultraviolet laser - induced fluorescence ( VUV -
LIF ) spectroscopy in a crossed beam configuration . -
pubmed
Histamine can exert effects through four different receptors , termed
H1R - H4R. -
pubmed
Intravenous injection of
histamine was sufficient to elicit these responses , and similar to
IgE - mediated
anaphylaxis , loss of both
H1R and
H2R was necessary for complete protection . -
pubmed
CONCLUSION : Our data demonstrate definitively that both
H1R and
H2R participate in the immediate systemic responses during
histamine - associated pathophysiology and mechanistically support the utility of
H2R - blocking therapeutics in alleviating symptoms of
anaphylaxis . -
pubmed
Disulfide bonds (
SS bonds ) are important
post - translational modifications of proteins . -
pubmed
Protein length also affects the
SS bond frequency : the average number of
SS bonds is positively correlated with the length for longer proteins ( & gt;200
amino acids ) , while for the shorter and less stable proteins ( &
lt;200
amino acids ) this correlation is negative . -
pubmed
Medium - sized proteins ( 250 - 350
amino acids ) indicated a high number of
SS bonds only in Archaea which could be explained by the need for additional protein stabilization in hyperthermophiles . -
pubmed
Denitrification activity was assayed using the
chloramphenicol amended
acetylene block method and bacterial communities were examined based on quantitative PCR and terminal restriction length polymorphisms of the
nitrous oxide reductase (
nosZ ) and
16S rRNA genes . -
pubmed
The aim of this study was to verify the clinical responses to Thai massage (
TM ) and Thai herbal compression ( THC ) for treating
osteoarthritis (
OA ) of the knee in comparison to oral
ibuprofen . -
pubmed
In conclusion ,
TM and
THC generally provided comparable clinical efficacy to oral
ibuprofen after three weeks of treatment and could be considered as complementary and alternative treatments for
OA of the knee . -
pubmed
The change in the hypothalamic neuropeptide balance in response to
leptin administration was associated with increased
oxygen consumption ,
carbon dioxide production , and physical activity , which resulted in increased milk intake ( PN14 ) with no change in
body weight . -
pubmed
Here we use
hydrogen /
deuterium exchange to examine the interactions between a panel of
bNAbs and native - like
Env trimers ( SOSIP.664 trimers ) . -
pubmed
In this study , we investigated the function of
6 - formylindolo ( 3,2 - b ) carbazole (
Ficz ) , a ligand of
AHR , on
IL-7 ,
colitis , and IEL phenotypes . -
pubmed
Ficz also decreased
CD8alphabeta(+ ) and
CD8(+ ) IEL subpopulations , enhanced
TCRgammadelta(+ ) IEL subpopulation , and reduced the percentage of activated
CD4(+ ) and
CD8(+ ) subpopulations . -
pubmed
UVA - induced ROS and comet - like DNA formation were remarkably suppressed by
EGT with a parallel inhibition of apoptosis , as evidenced by reduced DNA fragmentation ( TUNEL ) ,
caspase-9 /- 3 activation , and
Bcl-2 /
Bax dysregulation . -
pubmed
Dose - dependent increases of antioxidant genes ,
HO-1 ,
NQO-1 , and
gamma - GCLC and
glutathione by EGT were associated with upregulated
Nrf2 and downregulated
Keap-1 expressions . -
pubmed
Notably , augmented
luciferase activity of ARE may explain
Nrf2 / ARE - mediated signaling pathways behind
EGT dermato - protective properties . -
pubmed
This phenomenon was confirmed with suppressed nuclear
Nrf2 activation , and consequently diminished antioxidant genes in cells treated with respective pharmacological inhibitors (
LY294002 ,
GF109203X , and
N - acetylcysteine ) . -
pubmed
ERK1 and
ERK2 are the
effector kinases of the
ERK1 / 2 MAP - kinase signaling pathway , which plays a central role in transducing signals controlling cell proliferation , differentiation , and survival . -
pubmed
Serum levels of
PTH , ionised
calcium , and
creatinine were determined together with
glomerular filtration rate (
GFR ) . -
pubmed
On the contrary ,
nintedanib (
BIBF1120 ) was identified in large drug screening tests as a very specific inhibitor of certain
tyrosine kinases , but no published data on preclinical tests existed until 2014 . -
pubmed
perforatum extract ,
hypericin and
hyperforin for 24 and 72 h. Then , a real - time PCR analysis was accomplished after incubating the cells with these products evaluating the relative
CYP1A2 and
CYP2D6 expression . -
pubmed
Researchers at the University of California , Los Angeles , have shed light on mechanisms by which
melanoma cells become resistant to
PD-1 blockade with
pembrolizumab . -
pubmed
After 24 h treatment ,
cordycepin suppresses epithelial - mesenchymal transition and migration in
ovarian carcinoma cells through inhibiting
estrogen - related receptor ( ERR )- alpha . -
pubmed
RESULTS : All changes in temperature for HVS ,
GAL , and TENS therapeutic electrical currents observed in our model system fall below the predefined 3 C. CONCLUSION : To the best of our knowledge , this is the first experimental based observation that prolonged exposure to a direct electrical current at therapeutic strength does not result in heating of metal
titanium plates . -
pubmed
Nearly 80 % of
DIPGs harbor mutations in
histone H3 genes , wherein
lysine 27 is substituted with
methionine ( H3K27 M ) . -
pubmed
H3K27 M has been shown to inhibit
polycomb repressive complex 2 ( PRC2 ) , a multiprotein complex responsible for the methylation of
H3 at
lysine 27 ( H3K27me ) , by binding to its catalytic subunit
EZH2 . -
pubmed
The block of
P2X7R by
JNJ47965567 significantly ( p<0.05 ) reverted the damage elicited by high
glucose , detected as
IL-1beta and
LDH release . -
pubmed
Overall , our findings suggest that the
P2X7R represents an attractive pharmacological target to manage the early phase of
diabetic retinopathy , and the compound
JNJ47965567 is a good template to discover other
P2X7R selective antagonists . -
pubmed
bla OXA-48 carbapenemase was detected in seven O25b and one
O16 isolates ; of those , one O25b - ST131 isolate was
carbapenem - susceptible . -
pubmed
Metallocluster extrusion requirements , interspecies
MoFe - protein primary sequence comparisons and comparison of the primary sequences of the
MoFe - protein subunits with each other have been used to assign potential P - cluster (
Fe - S cluster ) domains within the
MoFe protein . -
pubmed
In each beta unit of the
MoFe protein , subunit domains , which include potential
Fe - S cluster ligands
Cys-62 ,
His-83 ,
Cys-88 and
Cys-154 , and beta - subunit domains , which include potential
Fe - S cluster ligands
Cys-70 ,
His-90 ,
Cys-95 and
Cys-153 , are proposed to comprise nearly equivalent P - cluster environments located adjacent to each other in the native protein . -
pubmed
Results of these experiments show that
MoFe - protein alpha - submit residues ,
Cys-62 and
Cys-154 , are probably essential for
MoFe - protein activity but that
His-83 and
Cys-88 residues are not . -
pubmed
A hypothesized mechanism for
everolimus - induced
bone pain includes marrow
edema and
hyperemia from reduced intraosseous perfusion , which have been previously reported in association with
mTOR inhibitor use . -
pubmed
Glucose ,
insulin or lipid levels remained low , without the development of
diabetes . -
pubmed
However , levels of
C - reactive protein (
CRP ) and
matrix metalloproteinase-3 (
MMP-3 ) gradually increased thereafter and
abatacept was initiated to prevent joint deterioration . -
pubmed
Cholera toxin (
CT ) is composed of a
disulfide - linked
A1 /
A2 heterodimer and a ring - like , cell - binding B homopentamer . -
pubmed
Reduction of the
A1 /
A2 disulfide bond is required for holotoxin disassembly , but reduced
CTA1 does not spontaneously separate from
CTA2 /
CTB5 :
protein disulfide isomerase (
PDI ) is responsible for displacing
CTA1 from its non - covalent assembly in the
CT holotoxin . -
pubmed
In contrast , high
HNF-1beta expression was significantly associated with
SLC3A1 expression , which plays a major role in
HNF-1beta - triggered induction of reactive
oxygen species in
OCCCas , independent of abnormalities in both
beta - catenin and MSI /
MMR status . -
pubmed
While it was long held that T cells were the primary mediators of
multiple sclerosis (
MS ) pathogenesis , the beneficial effects observed in response to treatment with Rituximab (
RTX ) , a
monoclonal antibody (
mAb ) targeting
CD20 , shed light on a key contributor to
MS that had been previously underappreciated : B cells . -
pubmed
Using two expression profiles downloaded from the Gene Expression Omnibus database , differentially expressed genes (
DEGs ) between responders and non - responders to
fluorouracil - based chemotherapy were identified . -
pubmed
Following feature selection using two methods , a random forest classifier for response to
fluorouracil - based chemotherapy with 13
DEGs was constructed . -
pubmed
Simulating
moxalactam dosage for extended - spectrum
beta - lactamase - producing Enterobacteriaceae using blood antimicrobial surveillance network data . -
pubmed
Objectives : Monte Carlo simulation (
MCS ) was used to evaluate optimal dosage for
cefepime (
FEP ) ,
moxalactam (
MOX ) , and
cefperazone /
sulbactam (
CFZ /
SBT ) against extended - spectrum
beta - lactamase (
ESBL ) producers isolated from the Blood Bacterial Resistant Investigation Collaborative System . -
pubmed
Conventional
MOX and
CFZ /
SBT doses failed to reach 90 %
PTA against isolates with MICs & gt;=8 mg / L and & gt;=4 mg / L , respectively . -
pubmed
Paracetamol treatment produced an elevation in serum
urea ,
uric acid ,
creatinine ,
C - reactive protein , renal
nitric oxide , and lipid
peroxide levels , and reductions in
interleukin-10 ,
superoxide dismutase , and
glutathione levels . -
pubmed
BACKGROUND : During the initial scale up of
ART in sub - Saharan Africa , prescribed regimens included drugs with high potential for
toxicity ( particularly
stavudine ) . -
pubmed
The median
BMI and
CD4 count were 21.9 ( IQR : 19.6 - 24.8 ) and 188 cell / microL ( IQR : 65 - 353 ) respectively ; 27.5 % were started on
stavudine , 46 % on
zidovudine , and 26.5 % on a
tenofovir containing regimens . -
pubmed
We observed 6248
ART modifications in 4868/14,261 patients ( 34.1 % ) ; 1615 were due to toxicity , 1077 to treatment failure , 1330 to contraindications , and 1860 patients following WHO recommendation of phasing out
stavudine and substituting with another NRTI . -
pubmed
The application of
norepinephrine ( NE ) , agonists of
alpha - and beta - adrenergic receptors (
ARs ) or intracardiac nerves stimulation induced spontaneous AP in a form of periodical bursts or continuous firing . -
pubmed
Acting through the
muscarinic receptor ,
ACh reduces the magnitude of the
potassium M - current , a hyperpolarizing current that builds up as neurons fire . -
pubmed
Successful Treatment of Cosmetic Eyebrow Tattoos in Fitzpatrick III -
IV With Picosecond ( 1,064 , 532 - nm )
Neodymium - Doped
Yttrium Aluminum Garnet Laser With a
Perfluorodecalin - Infused Patch : A Pilot Study . -
pubmed
METHOD : In this study , we screened a small library and found that a
flavonoid compound ( ZGM1 ) promoted the aggregation of
beta - amyloid (
Abeta ) . -
pubmed
Subsequently , we used dot blotting ( DB ) , transmission electron microscopy (
TEM ) , and
thioflavin T fluorescence ( ThT ) measurements to study the aggregation of
Abeta under the influence of
ZGM1 . -
pubmed
Enhanced Electrosynthetic
Hydrogen Evolution by
Hydrogenases Embedded in a Redox - Active Hydrogel . -
pubmed
Hydrogenase enzymes offer a pathway for bioelectrochemically producing
hydrogen that is advantageous over traditional platforms for
hydrogen production because of low overpotentials and ambient operating temperature and pressure . -
pubmed
We further show that
Cc - PAA - mediated
hydrogenases can operate at high faradaic efficiency ( 80 - 100 % ) and low overpotential ( -0.578 to -0.593 V vs.
SHE ) . -
pubmed
Specific activities of these
hydrogenases in the electrosynthetic
Cc - PAA assay were comparable to their respective activities in traditional
methyl viologen assays , indicating that
Cc - PAA mediates electron transfer at high rates , to most of the embedded enzymes . -
pubmed
Interestingly , the
PEI -
PLA copolymer could effectively bind siRNA targeting
PKM2 , and the obtained polyplex displayed much higher stability in serum than naked siRNA as determined by
agarose gel electrophoresis . -
pubmed
A diverse variety of
CAMs assemble into complexes with
glutamate and
GABA receptors and regulate the targeting of these receptors to the cell surface and synapses . -
pubmed
Relating to the molecular mechanism in
Iron Deficiency Anemia (
IDA ) ,
hepcidin has a key role in
iron homeostasis . -
pubmed
It downregulates the
iron exporter
ferroportin , inhibiting both
iron absorption and release . -
pubmed
It can be used safely in various locations and different indications for preventingcomplications such as preventing dehiscence in revision surgeries , cerebrospinal fluid (
CSF )
fistula formation in the scalp , and
wound breakdown in chronic
corticosteroid use . -
pubmed
Participants completed a laboratory - based psychosocial stress paradigm ( the Trier Social Stress Test - TSST ) pre- and
post - intervention , throughout which
cortisol and cardiovascular profiles were measured . -
pubmed
Neither
cortisol nor cardiovascular reactivity to the TSST was changed pre - to
post intervention , either between or within groups . -
pubmed
Reduction of
CD73 protein resulted in significant suppression of
GSC viability , proliferation and clonogenicity , whereas
CD73 enzymatic activity exhibited negative effects only on
GSC invasion involving impaired downstream
adenosine (
ADO ) signalling . -
pubmed
Furthermore , application of
phosphodiesterase inhibitor
pentoxifylline , a potent immunomodulator , effectively inhibited
ZEB1 and
CD73 expression and significantly decreased viability , clonogenicity , and invasion of
GSC in vitro cultures . -
pubmed
Given the involvement of
adenosine and
A3 adenosine receptor in
GSC invasion , we investigated the effect of the pharmacological inhibition of
A3AR on
GSC maintenance . -
pubmed
L - NAME ( non - selective
nitric oxide synthase [
NOS ] inhibitor ) ,
aminoguanidine ( inducible
NOS inhibitor ) , and
7 - nitroindazole ( neuronal
NOS inhibitor ) were administered prior to
modafinil . -
pubmed
The main outcome variables included the percentage of
necrotic area ( PNA ) in flap tissues , histopathological results ,
vascular endothelial growth factor (
VEGF ) immunohistochemical ( IHC ) staining , and
nitrite concentrations . -
pubmed
All
NOS inhibitors significantly reversed the protective effect of
modafinil ( p < 0.001 ) . -
pubmed
Root canal filling was performed by the lateral compaction technique , using gutta - percha and an
epoxy resin - based sealer (
AH Plus ) labeled with 0.1 %
Rhodamine B dye . -
pubmed
Ethanol - induced changes in activities of
adenylate cyclase ,
guanylate cyclase and
cyclic adenosine 3 ' , 5 '- monophosphate dependent protein kinase in the brain and liver . -
pubmed
Maximum stimulation occurred at four days of incubation and 18 hours of
pulse labeling with
tritiated thymidine using 50 micrograms per milliliter of
phytohemagglutinin and 1 microcurie of
tritiated thymidine . -
pubmed
The disruption of intercellular contacts , which accompanies cell dissociation and acquisition of motility , is correlated with a redistribution of
E - cadherin , a
Ca ( 2 +)-dependent cell adhesion molecule , over the entire cell surface and within the cytoplasm . -
pubmed
The hemodynamic effects of
ANF infusion ( 100 micrograms / h for 60 min ) were also examined in animals pretreated with the
angiotensin - converting enzyme ( ACE ) inhibitor ,
captopril . -
pubmed
Infusion of
ANF attenuated the pressor responsiveness to exogenous
AII and NE , but caused no significant change in the blood pressure increases produced by
vasopressin . -
pubmed
Infusion of
captopril increased plasma concentration of
renin ( PRC ) , and infusion of
ANF produced no further change in PRC . -
pubmed
The fragments were electrophoresed , transferred onto a
nitrocellulose membrane and hybridized with two specific probes : pC21a for the
CYP21 genes and pAT - A for the
C4 genes . -
pubmed
Two main types of
CGD are known , an X - linked form which is normally associated with the absence of
cytochrome b558 , a component of the
membrane - associated reduced nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase which generates
superoxide and an autosomal recessive form , in which
cytochrome b558 is present , caused by the deficiency of a cytosolic factor required to activate
NADPH oxidase . -
pubmed
The origin of cerebellar
NPY and possible colocalization with
norepinephrine are discussed . -
pubmed
The second example presents the balance of
haemophilia A carrier detection rate performed in 30 obligatory carriers and 42 normal women by examination of F.VIII : C and
VWF -
Ag using universal discriminant . -
pubmed
We conclude that immunocytochemistry on
PLL - coated slides constitutes the method of choice for immunologic cell differentiation in
CSF , which allows equivocal morphologic findings to be clarified . -
pubmed
Although the receptor - mediated
LDL uptake appeared to exert a strong influence on
bile acid - independent bile flow , the receptor - independent uptake showed a significant relation with biliary
bile acid excretion . -
pubmed
Differences between the two mechanisms of
LDL uptake were also evident in the biliary
bile acid -
cholesterol coupling , which was significantly stronger during receptor - independent than during receptor - dependent uptake of LDL . -
pubmed
The effects of
LDL uptake on bile secretion were modulated by the experimentally induced changes in both the content and composition of
bile acids in the enterohepatic circulation . -
pubmed
Cytochalasin B and
Colchicine differ in their effect on the release of charge changing
lymphokines . -
pubmed
The
amino acid composition and the
amino - terminal sequence of the enzyme indicate that the protein is related to
human pancreatic ribonuclease and to
angiogenin , and that it may be identical with an eosinophil - derived neurotoxin and to a
ribonuclease that has been isolated from urine . -
pubmed
The catalytic activity of the
liver ribonuclease and its sensitivity to
iodoacetic acid inactivation also relate the enzyme to the
pancreatic RNases , but the liver protein is clearly differentiated by immunological measurements . -
pubmed
These include panretinal photocoagulation (
PRP ) , using the argon laser or
xenon arc , for
proliferative retinopathy , and focal photocoagulation for
macular oedema . -
pubmed
Other therapeutic modalities , such as hypophysectomy for severe
retinopathy , remain controversial , while still others , such as rigorous blood
glucose control and
aldose reductase inhibitors , are currently under investigation . -
pubmed
coli host cells ,
exonuclease III , was not utilized in repair of lethal
H2O2 damage to the phage . -
pubmed
HbO2 produced a dose - dependent reduction in
AChE activity . -
pubmed
In this concentration range
MetHb and
PSA had little effect on
esterase activity . -
pubmed
Estrogen and
progesterone receptor content in
breast tumor cytosols calculated with a pocket calculator . -
pubmed
Activation of
methionine synthetase by a reduced
triphosphopyridine nucleotide - dependent flavoprotein system . -
pubmed
With
UDP - glucuronic acid prolonged incubation and variation of the composition of the incubation mixtures yielded equimolar amounts of
azodipyrrole ( I ) and
azodipyrrole beta - d - monoglucuronoside (
II ) after treatment of the incubation mixtures with the
diazonium salt of
ethyl anthranilate . -
pubmed
After ammonolysis the conjugated
azo - derivative (
II ) yielded
d - glucuronic acid and the
carboxylic acid amide of azodipyrrole , indicating transfer of a
glucuronic acid residue to the
carboxylic acid groups of
bilirubin . -
pubmed
The determination of
human serum albumin by its specific binding of the anionic dye ,
2 -( 4 '- hydroxybenzeneazo )- benzoic acid . -
pubmed
By indirect immunofluorescence , the type 1 specific ( 6D4 ) and the group specific ( 1B5 ) reacted with a cytoplasmic
antigen while the type 2 specific (
G3 ) reacted with a nuclear
antigen . -
pubmed
Mn2 + activated the adult ox enzyme 9 - fold and calf
enzyme 22 - fold under optimal conditions ( no
KCl ) . -
pubmed
The
vitamin K - dependent carboxylase from the
dicoumarol - treated calf is apparently more tightly bound to the microsomal membrane than is the adult ox enzyme . -
pubmed
Streptozotocin diabetes also caused a decrease in the cross - linking of reparative
collagen as judged by the ratio of breaking strengths of skin incisions before and after
formalin fixation . -
pubmed
No significant associations of
HLA - A or - B antigens with either group were observed after correction for the number of
antigens tested . -
pubmed
In the present report we show that
protein kinase C (
PKC ) activity could be measured in extracts of distal neurites by an assay of the
Ca ( 2 +)-dependent phosphorylation of a
PKC - specific octapeptide substrate . -
pubmed
We found that local application of 1 microM
PMA for 24 h to distal neurites caused nearly complete down - regulation of
Ca ( 2 +)-dependent
PKC activity measured in this manner . -
pubmed
We then investigated the effects of the recently available
PKC inhibitors ,
calphostin C and
chelerythrine , finding that , like
PMA , these inhibited the growth of distal neurites when applied locally to them , and had no effect when applied to cell bodies and proximal neurites . -
pubmed
However , the inhibition of neurite growth by
calphostin C occurred at a concentration far below its IC50 value for
protein kinase inhibition , and both
calphostin C and
chelerythrine inhibited distal neurite growth even in neurons pretreated with
PMA . -
pubmed
[ Study of the substrate specificity of
T4 RNA ligase : interaction of the enzyme with
ATP analogs modified through the
ribose residue and with affinity sorbents ] . -
pubmed
To elucidate the role of the
ribose moiety in substrate binding , various
ATP derivatives modified in
ribose moiety were studied as probes for the
T4 RNA ligase first stage reaction . -
pubmed
Adenosine triphosphates attached to
agarose were used as affinity adsorbent for purification of
T4 RNA ligase . -
pubmed
D1 ,
CDDP 100 mg / m2 ip
D1 ,
CBDCA 300 mg / m2 i.v . -
pubmed
The predicted
amino acid sequence shows extensive homology to
vitellogenin , the precursor of the lipovitellin complex , which has been shown by X - ray crystallography to contain a large lipid storage cavity . -
pubmed
Results of segregation analysis are reviewed for six possibly related traits ( high
sodium -
lithium countertransport , low urinary
kallikrein excretion , high fasting plasma
insulin level , a fat pattern index , dense
LDL subfractions , and
body mass index ) . -
pubmed
Oxygen sensitivity of second - phase secretion rate declined after 1 wk of in vitro culture :
P50 was 13 mmHg after 1 wk and remained at 10 mmHg after 2 - 5 wk of culture . -
pubmed
The reduction in
insulin secretion is thought to be associated with the existence of
pO2 gradients outside and inside the isolated islets , resulting in exposure of islet cells to low pO2 levels that decrease radially from the periphery to the core . -
pubmed
Additionally , thromboelastography ,
prothrombin time , partial
thromboplastin time , activated clotting time ,
fibrinogen , platelet counts , and hematocrit values were evaluated before the operation , after
heparin reversal , after infusion of platelet rich plasma or control solution , and 2 hours after infusion . -
pubmed
Perinatal hypothyroidism induced by
methimazole treatment did not significantly alter the appearance of cerebral cortical
G alpha o1 and
G alpha o2 between days 1 and 22 postpartum . -
pubmed
The
boIL-5 coding sequence is 405 bp long , coding for a 15.2 kDa precursor protein of 134
amino acids ( aa ) . -
pubmed
Interleukin-1 (
IL-1 ) and
ibuprofen modulate the host response in different models after endotoxic challenge . -
pubmed
Serum levels of
IL-1 alpha ,
tumor necrosis factor - alpha (
TNF alpha ) , and
interleukin-6 (
IL-6 ) were determined 1,2 , and 4 hr ,
post - LPS , and
prostaglandin E2 (
PGE2 ) urine levels 4,8 , and 12 hr
post - LPS , and a comparative mortality study was performed . -
pubmed
IL-1 beta treatment provoked a reduction of
IL-1 alpha ,
TNF alpha , and
IL-6 without affecting
PGE2 , while
ibuprofen provoked a later increase of
IL-1 alpha ,
TNF alpha , and
IL-6 , with a decrease of
PGE2 . -
pubmed
Intravenous doses were as follows :
rt - PA , 5 mg / kg over 0.5 h with 20 % of the total dose given as a bolus ;
hirulog , 1 mg / kg bolus followed by 5 mg / kg over 1 h. MRI was performed at 2 , 3 , and 5 h following embolization . -
pubmed
Significant
cerebral hemorrhage was observed in one placebo , two
rt - PA , and three
rt - PA plus
hirulog treated animals . -
pubmed
We conclude that
rt - PA plus
hirulog improves cerebral perfusion but does not necessarily reduce
cerebral injury . -
pubmed
At this point the nuclear envelope becomes freely permeable to large ligands , as indicated by experiments with
digitonin - treated cells and by the massive release of solubilized
A - type lamins into the cytoplasm . -
pubmed
M3S contains four changes : a hydrophilic substitution of L29S , two hydrophobic substitutions of S52I and Y56F , and a deletion of the N - terminal seven
amino acids that is disordered in the structure of
wild - type TNF - alpha . -
pubmed
The low systemic
toxicity of
M3S appears to be the effect of the reduced and selective binding affinities for the
TNF receptors , and the superior
tumor - suppression of M3S appears to be the effect of its weak but longer antitumoral activity in vivo compared with wild - type
TNF - alpha . -
pubmed
Significantly high
triglycerides and total
cholesterol were observed in Groups I and
II ( p & lt ; 0.001 ) and low
high - density lipoprotein cholesterol in Group
II ( p & lt ; 0.001 ) , when compared to controls . -
pubmed
At 60 minutes during oral
glucose tolerance test , higher
insulin levels were seen in Groups I and
II as compared to controls ( p =
NS ) . -
pubmed
Secreted pro -
Atl ( 1256
amino acids ) is cleaved at residues 198 and 775 to generate a pro - peptide ,
amidase and
glucosaminidase , respectively . -
pubmed
Cells electroporated in the presence of
anti - CaM antibodies had reduced influx of extracellular
Ca2 + , with no change in release of
Ca2 + from the internal stores . -
pubmed
These observations suggest that T cell receptor engagement initiates
Ca2 + influx by a pathway that likely includes
CaM , which may in turn regulate
IT . -
pubmed
Influx of extracellular
Ca2 + is required for cellular proliferation , and inhibition of
CaM by pharmacological inhibitors reduced cellular proliferation . -
pubmed
A combination of serum
IgG precipitation by
ammonium sulfate and yolk
IgG extraction from egg was loaded in
sodium dodecyl sulfate - polyacrylamide gel upon which the
IgG was resolved by electrophoresis . -
pubmed
Serum PRL and the concentrations of
catecholamines ,
indoleamine , and their metabolites were measured by RIA and HPLC , respectively . -
pubmed
Treatment with 5.0 mg
deprenyl decreased the
tumor diameter ,
tumor number , and
serum prolactin (
PRL ) level . -
pubmed
Treatment with 5.0 mg
deprenyl enhanced the concentrations of
norepinephrine ( NE ) and
serotonin ( 5 -
HT ) in the MBH and in the ST , and the concentration of
dopamine ( DA ) in the MBH . -
pubmed
These results suggest that the suppression of the development and growth of
DMBA - induced
mammary tumors by chronic
deprenyl treatment may be mediated through alterations in the synthesis and metabolism of
catecholamines and
indoleamine in the MBH and inhibition of
PRL secretion . -
pubmed
Signal cross - contamination was approximately 0.2 % between individual
coils , and simultaneous one- and two - dimensional spectra have been obtained from samples of
fructose ,
galactose ,
adenosine triphosphate , and
chloroquine ( 7 nmol of each compound ) . -
pubmed
Since we had shown that Delta(2,4 ) double bonds could not be metabolized extra - peroxisomally to restore growth of the
sps19Delta strain , we postulate that
rat 3,2 - isomerase acted on the Delta(3 ) unsaturated metabolite of
oleic acid by replacing the mutant 's missing activity from within the peroxisomes . -
pubmed
These compounds were released enzymatically from their precursors by percolating the must through an immobilized
tryptophanase column (
EC 4.1.99.1 ) , catalyzing an alpha , beta - elimination reaction on the
S - cysteine conjugate . -
pubmed
The volatile
thiols were analyzed by
GC -
MS , as were the deuterated analogues that had been released from synthesized deuterated precursors and were added as internal standards . -
pubmed
Ankyrins are spectrin - binding proteins that associate via
ANK repeats with a variety of ion channels / pumps ,
calcium release channels and cell adhesion molecules . -
pubmed
It consists of infinite , negatively charged , square , parallel columns bundled through
N - H ...
NC hydrogen bonds between
NH ( 4 )(+ ) and terminal
CN from the columns . -
pubmed
Bicapped ( square
face ) trigonal prismatic coordination geometries were observed for
Ln ( III ) centers in 1 and 2 . -
pubmed
Our previous work has identified two separate DNA - binding regions on
NM23 - H2 / PuF : a sequence - dependent DNA - binding surface involving residues
Arg34 ,
Asn69 , and
Lys134 on the equator of the hexameric protein and a covalent DNA - binding site involving
Lys12 located in the
nucleotide - binding site , the site of the
NDP kinase reaction . -
pubmed
To understand the role of the
nucleotide - binding site in the DNA cleavage reaction and to establish a connection between the
nuclease and the
NDP kinase activities , we used the known crystal structure of
NM23 - H2 complexed with
GDP as the basis for site - directed mutagenesis . -
pubmed
We thus identified
Arg88 and
Arg105 as residues that are , in addition to
Lys12 , critical for covalent DNA binding and DNA cleavage , as well as for the
NDP kinase reaction . -
pubmed
Another residue ,
Gln17 , was required only for DNA cleavage , and
Tyr52 ,
Asn115 , and
His118 were found to be essential only for the
NDP kinase activity . -
pubmed
We also show that
nucleoside triphosphates but not
nucleoside diphosphates inhibited the covalent DNA binding and DNA cleavage reactions , independent of
phosphoryl transfer and the
NDP kinase reaction . -
pubmed
These findings collectively suggest that the binding modes of
mononucleotides and duplex DNA oligonucleotides in the
nucleotide - binding site differ , and that
NM23 -
H2 possesses multiple biochemical activities . -
pubmed
Yolk
oestradiol (
E2 ) increased seasonally while
testosterone ( T ) decreased . -
pubmed
For that purpose , we used
WY14643 [
4 - chloro-6 -( 2,3 - xylidino )- 2 - pyrimidinylthio acetic acid ] as a
PPARalpha agonist ,
L-165041 and
L-783483 as
PPARbeta ligands , and
15 - deoxy - Delta ( 12,14 )- PGJ2 (
15d - PGJ2 ) ,
troglitazone , and
ciglitazone for
PPARgamma . -
pubmed
Cell viability against
glutamate ,
hydrogen peroxide (
H ( 2 ) O ( 2 ) ) , and serum deprivation insults was determined using a
calcein acetoxymethyl (
AM ) assay . -
pubmed
However , the neuroprotective effects of those compounds are unlikely to be mediated through the
PPARgamma for two reasons : ( 1 ) various concentrations of another
PPARgamma agonist (
ciglitazone ) were not neuroprotective ; ( 2 ) by itself ,
PPAR exhibits a low affinity for DNA , and high - affinity binding requires heterodimerization with
RXR , the
9 - cis - retinoic acid receptor ; administering
9 - cis - retinoic acid in conjunction with
15d - PGJ2 did not alter the neuroprotective effects of the latter . -
pubmed
Our results demonstrate neuroprotective effects of
15d - PGJ2 and
troglitazone that are likely independent of
PPARgamma . -
pubmed
Mechanical load significantly increased
procollagen alpha1 ( I ) mRNA levels up to twofold above static controls after 24 h. This increase was completely abolished by the
MEK 1 / 2 inhibitor
U0126 , with no effect on basal levels . -
pubmed
In contrast ,
SB203580 , a specific inhibitor of
p38MAPK , enhanced both basal and stretch - stimulated levels of procollagen mRNA . -
pubmed
Consistent with this finding , selective activation of the
p38MAPK signalling pathway by expression of
MKK6(
Glu ) , a constitutive activator of
p38MAPK , significantly reduced
procollagen alpha1 ( I ) promoter activity . -
pubmed
SB203580 - dependent increase in
procollagen alpha1 ( I ) was accompanied by
ERK 1 / 2 activation , and inhibition of this pathway completely prevented
SB203580 - induced
procollagen alpha1 ( I ) expression . -
pubmed
Furthermore , the LC - ESI -
TOF -
MS analyses revealed that
HALS-1 ( oligomeric ) was highly complex and consisted of at least five different mass series , while
HALS-2 , which was assumed to be monomeric , contained two different mass series . -
pubmed
In these studies , multiple conformations of the
RNA polymerase II elongation complex were revealed by their distinct elongation potential and differing dependence on
nucleoside triphosphate substrate . -
pubmed
Using
alpha - amanitin as a dynamic probe of the
RNA polymerase II mechanism , we show that the most highly poised conformation of the elongation complex , which we interpreted previously as the posttranslocated state , is selectively resistant to inhibition with
alpha - amanitin . -
pubmed
This reaction , which is catalyzed by the
protein - tyrosine kinase YwqD , activates the enzyme , while dephosphorylation of
phosphotyrosine -
YwqF by the
phosphotyrosine - protein phosphatase YwqE reduces its enzyme activity . -
pubmed
The
UDP - glucose dehydrogenase reaction catalyzed by
YwqF is inhibited by one of its substrates ,
UDP -
glucose , and the extent of this inhibition seems to be reduced upon
YwqF phosphorylation . -
pubmed
We propose that this effect could at least partly account for the observed activation of
YwqF induced by
tyrosine phosphorylation . -
pubmed
PURPOSE : In this study , we investigated the antitumor efficacy of
thrombospondin-1 three type 1 repeats ( 3TSR ) , the antiangiogenic domain of
thrombospondin-1 , in comparison and in combination with
gemcitabine , in an orthotopic
pancreatic cancer model . -
pubmed
METHODS : In 2002 , we studied prescribing patterns of beta - blockers ,
calcium channel blockers ,
angiotensin converting enzyme ( ACE ) inhibitors ,
angiotensin - II antagonists and statins in the County of Funen , Denmark ( 180 general practices , 472,000 inhabitants ) . -
pubmed
The overall structure of
IDO shows two alpha - helical domains with the
heme between them . -
pubmed
A mutant analysis shows that none of the polar
amino acid residues in the distal
heme pocket are essential for activity , suggesting that , unlike the
heme - containing
monooxygenases ( i.e. -
pubmed
,
peroxidase and
cytochrome P450 ) , no protein group of
IDO is essential in
dioxygen activation or proton abstraction . -
pubmed
These characteristics of the
IDO structure provide support for a reaction mechanism involving the abstraction of a proton from the substrate by
iron - bound
dioxygen . -
pubmed
This study provides the structural basis for a
heme - containing
dioxygenase mechanism , a missing piece in our understanding of
heme chemistry . -
pubmed
Microtiter plates were sensitized with
tumor necrosis factor alpha (
TNF - alpha ) and saturated with
phosphate - buffered saline (
PBS ) containing 1 %
bovine serum albumin (
BSA ) . -
pubmed
Pfs expression is required for several metabolic pathways and limits the production of
autoinducer-2 , a
molecule proposed to play a central role in interspecies quorum sensing . -
pubmed
The negative element is contained within the overlapping
dgt promoter , which is involved in
purine metabolism . -
pubmed
Oxygenase enzyme activity was determined in vivo using BD
Oxygen Biosensor plates to measure
oxygenase - mediated
oxygen depletion in the presence of specific aromatic analytes . -
pubmed
No significant signals were detected with
hydrocarbons that were not substrates of the initial
oxygenases . -
pubmed
Thus ,
ethanol appears to prevent perseveration in performing needless instrumental behavior induced by repeated activation of
D2 / D3 receptors . -
pubmed
Apart from true double sensitization ,
IgE against cross - reactive
carbohydrate determinants ( CCDs ,
alpha1,3 - fucosylated N - glycans ) with low clinical relevance is the most frequent and often only cause for the multiple reactivity . -
pubmed
METHODS : Both gastroduodenal and small
IP were assessed using an established three -
sugar test , where urinary
disaccharide excretion reflecting intestinal uptake was measured using HPLC . -
pubmed
At the beginning and at the end of the study we recorded some clinical indicators such as
BMI and WHR , some hormonal indicators such as
testosterone ,
insulin ,
SHBG and
DHEAS , as well as some metabolic indicators such as total
cholesterol ,
triglycerides and
HDL - C. RESULTS : We discovered a considerable reduction of 27,1 % in
insulin and HOMA - IR ( 29,1 % ) , which indicates an improved
insulin resistance . -
pubmed
The decrease in
testosterone and the increase in
SHBG were not statistically significant . -
pubmed
Although its mode of action is still not fully understood ,
ATO is known to induce cell apoptosis via generation of reactive
oxygen species and activation of
caspases . -
pubmed
Photodynamic effects of
steroid - conjugated fluorophores on
GABAA receptors . -
pubmed
We have shown that fluorescent ,
7 - nitro-2,1,3 - benzoxadiazol-4 - yl amino (
NBD)-conjugated
neurosteroid analogs photopotentiate
GABA ( A ) receptor function . -
pubmed
Our results are generally in agreement with the idea that
steroids with activity at
GABA ( A ) receptors are the most potent photopotentiators . -
pubmed
Remarkably the crystal structure of this protein , solved to 1.2 A using x - ray crystallography , revealed that
YtfQ binds a single molecule of
galactofuranose in its ligand binding pocket . -
pubmed
Modulatory effects of
cAMP and
PKC activation on gap junctional intercellular communication among thymic epithelial cells . -
pubmed
BACKGROUND : We investigated the effects of the signaling molecules ,
cyclic AMP (
cAMP ) and
protein - kinase C (
PKC ) , on gap junctional intercellular communication ( GJIC ) between thymic epithelial cells (
TEC ) . -
pubmed
RESULTS : Treatment with
8 - Br - cAMP , a
cAMP analog ; or
forskolin , which stimulates
cAMP production , resulted in an increase in dye transfer between adjacent
TEC , inducing a three - fold enhancement in the mean fluorescence of coupled cells , ascertained by flow cytometry after
calcein transfer . -
pubmed
VIP ,
adenosine , and
epinephrine which may also signal through
cyclic nucleotides were tested . -
pubmed
Stimulation of
PKC by
phorbol - myristate - acetate inhibited inter - TEC GJIC . -
pubmed
CONCLUSIONS : Overall , our data indicate that
cAMP and
PKC intracellular pathways are involved in the homeostatic control of the gap junction - mediated communication in the thymic epithelium , exerting respectively a positive and negative role upon cell coupling . -
pubmed
Fibroblastoid mast cells demonstrated increased
chymase expression and activation with exaggerated spontaneous
histamine release . -
pubmed
A moderate increase of
VEGF - C secretion was also observed in the presence of hypertonic doses of
NaCl , which mimicked the matrix of chitin hydrolysate stock solution , and in the presence of
chitin - binding lectin ,
WGA (
wheat germ agglutinin ) . -
pubmed
These results suggest a novel function of chitin - derived
oligosaccharides as
VEGF - C stimuli . -
pubmed
Since anti - apoptotic effect of
ERK has not been elucidated clearly in DNA - damage - induced cell
death , the role of
ERK was examined in normal HEF cells treated with mild DNA damage using
etoposide or
camptothecin . -
pubmed
PD98059 increased apoptosis and reduced DNA - damage - induced
p21Waf1 /
Cip1 /
Sdi level . -
pubmed
Depletion of
p21Waf1 /
Cip1 /
Sdi induced cell
death and
PD98059 induced additional cell
death . -
pubmed
DNA - damage - induced increase in cytoplasmic localization and phosphorylation of
threonine residues of
p21Waf1 /
Cip1 /
Sdi was reversed by
PD98059 . -
pubmed
The histological grade and pyramidal neuronal density in the
CA1 hippocampus were measured to quantitate the degree of
hippocampal injury under
thionin staining . -
pubmed
Since endothelium - derived
nitric oxide (
NO ) suppresses constitutively the release and vascular effects of
thromboxane A ( 2 ) (
TXA ( 2 ) ) ,
NO - deficiency is often associated with activation of
thromboxane receptors (
TP ) . -
pubmed
In the present study we hypothesized that in the absence of
NO , overactivation of the
TP - receptor mediated cerebrovascular signaling pathway contributes to the development of vasomotion and
CBF oscillations . -
pubmed
Administration of the
TP - receptor agonist
U-46619 ( 1 microg / kg i.v . ) -
pubmed
U-46619 significantly augmented the
CBF - oscillations induced by
L - NAME while inhibition of endogenous
TXA ( 2 ) synthesis by
ozagrel ( 10 mg / kg i.v . ) -
pubmed
In isolated MCAs
U-46619 in a concentration of 100 nM , which induced weak and stable contraction under physiological conditions , evoked sustained vasomotion in the absence of
NO , which effect could be completely reversed by inhibition of
Rho - kinase by 10 microM
Y-27632 . -
pubmed
CONCLUSION / SIGNIFICANCE : These results suggest that
hypersensitivity of the
TP - receptor -
Rho - kinase signaling pathway contributes to the development of low frequency cerebral vasomotion which may propagate to
vasospasm in pathophysiological states associated with
NO - deficiency . -
pubmed
Low
HBsAg levels during
peginterferon alfa-2a therapy were associated with high rates of posttreatment response . -
pubmed
We demonstrate that electrospray ionization tandem ( triple quadrupole ) mass spectrometry ( ESI -
MS /
MS ) is an efficient method for monitoring
steryl glucosides ( SG ) and
acyl steryl glucosides (
ASG ) . -
pubmed
These data demonstrate that ESI -
MS /
MS response per molar amount of
sterol conjugate is similar among various molecular species of SG and
ASG . -
pubmed
METHODS : A cross sectional study was conducted in the north - east coast of Tanzania to investigate whether the changes in
glycosylphosphatidylinositol ( GPI)-anchored
complement regulatory proteins (
CD55 and
CD59 ) contributes to
malaria anaemia . -
pubmed
In this case , the dissolution of
IND ASD from water - soluble
PVP and
HPMCAS is almost instantaneous , resulting in an initial surge of
IND concentration followed by a sharp decline due to the nucleation and crystallization events triggered by the rapid build - up of drug supersaturation . -
pubmed
On the other hand , the dissolution of
IND ASD from insoluble crosslinked
PHEMA hydrogel beads is less rapid as it is regulated by a feedback - controlled diffusion mechanism , thus avoiding a sudden surge of supersaturation in the dissolution medium . -
pubmed
The absence of an apparent decline in drug concentration during dissolution from IND -
PHEMA ASD further reflects the diminished nucleation and crystallization events during IND dissolution from hydrogel - based solid molecular dispersions . -
pubmed
Also , by selecting the appropriate particle sizes , immediate to controlled release of
IND from IND -
PHEMA ASD can be readily achieved as the release rate increases with decreasing
PHEMA bead size . -
pubmed
Furthermore , a robust physical stability has been demonstrated in IND -
PHEMA ASD with no drug precipitation for up to 8 months at IND loadings below 16.7 % under direct open cup exposure to accelerated stability conditions ( 40 C/75 % RH ) . -
pubmed
After acquisiting DICOM data of PET /
CT / MR ( directed by radiotracer
18F - FDG etc . ) -
pubmed
The results showed that the
n - butanol extract had the highest anti - oxidant activity , and its anti - oxidant compounds were analysed by HPLC - DAD - ESI / MS(n ) and HPLC coupled with a
post - column derivatisation (
PCD ) system supplied with
DPPH ,
aluminium chloride or
sodium acetate solutions . -
pubmed
Results were classified as : normal
glucose tolerance ( NGT ) or
AGT (
impaired glucose tolerance [
IGT ] ,
CF related
diabetes [
CFRD ] or
indeterminate glucose tolerance [ INDET ] ) . -
pubmed
We analyzed : OGTT (
glucose and
insulin levels ) , nutritional status (
BMI -
SD ) and lung function ( forced spirometry ) . -
pubmed
In addition , r(m ) was modelled on the basis of detailed anatomical measurements to separate the importance of different serial resistances affecting
CO ( 2 ) diffusion into
chloroplasts . -
pubmed
Calculated charge densities indicate that electrophiles should attack at
C3 or
O and nucleophiles at C2 or C4 in broad agreement with experimental observations . -
pubmed
Complete inhibition of the
enzyme by the inhibitor
pepstatin A and the internal
amino acid sequence classified the protein as an
aspartic protease . -
pubmed
The
enzyme limitedly hydrolysed
myoglobin between 1 - Met and
2 - Gly , 43 - Lys and 44 - Phe , and 70 - Leu and 71 - Thr . -
pubmed
10 days after the start of using
spironolactone and
dabigatran bleeding was marked with decrease in
hemoglobin levels to 69 g / l ,
creatinine level increases to 3.6 mg / dL (
glomerular filtration rate by MDRD 18 ml / min/1,73 m2 ) , and
potassium to 5.5 mEq/ l. Against the background of the abolition of drugs normalization of renal function was marked . -
pubmed
A common mechanism is the overexpression of
ATP - binding cassette ( ABC ) efflux transporters in
cancer cells such as
P - glycoprotein (
P - gp /
ABCB1 ) ,
multidrug resistance - associated protein 1 (
MRP1 /
ABCC1 ) and
breast cancer resistance protein (
BCRP /
ABCG2 ) that limit the exposure to anticancer drugs . -
pubmed
PP ( III ) is neither a substrate nor an inhibitor of
pyrophosphatase , the
enzyme that efficiently catalyses the hydrolysis of
PP ( V ) . -
pubmed
Toward one -
pot lipase - catalyzed synthesis of
poly ( epsilon - caprolactone ) particles in aqueous dispersion . -
pubmed
The nature and amount of
lipase , as well as the nature of the support material were identified as key parameters controlling ring - opening polymerization of
epsilon - CL in aqueous dispersion . -
pubmed
The
body weight increase induced by
avosentan was associated with
CHF development ( P interaction=0.04 ) , whereas
hemoglobin decrease was not ( P interaction=0.64 ) . -
pubmed
Initially and following 4 weeks of
tolcapone intake , the neurologist scored with Unified
Parkinson ' s Disease Rating Scale parts I ,
II ,
IV , the nonmotor symptoms scale for
Parkinson ' s disease and recorded the off time . -
pubmed
Facile synthesis of brush
poly ( phosphoamidate ) s via one -
pot tandem ring - opening metathesis polymerization and atom transfer radical polymerization . -
pubmed
Poly ( 2 -( dimethylamino ) ethyl methacrylate ) (
PDMAEMA)-based brush
poly ( phosphoamidate ) s are successfully synthesized by a combination of ring - opening metathesis polymerization ( ROMP ) and atom transfer radical polymerization ( ATRP ) following either a commutative two - step procedure or a straightforward one -
pot process using
Grubbs ruthenium - based catalysts for tandem catalysis . -
pubmed
Proton pump inhibitors
omeprazole and
lansoprazole contain chiral
sulfur atom and they are administered as a racemate , i.e. -
pubmed
After 12 months , there were significant differences in the serum
25 ( OH ) D concentration ( mean difference : 49.54 ; 95 % CI : 43.94 to 55.14 ) nmol / l ) and
PTH levels ( mean difference : -1.02 ; 95 % CI : -1.67 to -0.38 pmol / l ) in the intervention group compared to placebo group . -
pubmed
There was significant difference between treatment group in both serum
25 ( OH ) D and
PTH . -
pubmed
Here , we utilized a NOE - based approach to assign ( stereo- ) specifically labeled
Ile ,
Leu ,
Val and
Ala methyl labeled and uniformly
( 15 ) N - Phe and
( 15 ) N - Tyr labeled
OmpX and calculated a refined high - resolution structure . -
pubmed
We further used NOE information between protonated lipid head groups and side - chain
methyls to determine the position of
OmpX in the phospholipid bilayer . -
pubmed
The protective
HO-1 effects on placentation and fetal growth can be mimicked by the exogenous administration of
carbon monoxide (
CO ) , a product of
heme catalyzed by
HO-1 . -
pubmed
PURPOSE :
Telaprevir inhibits
CYP3A resulting in drug - drug interactions ( DDI ) of unprecedented magnitude . -
pubmed
METHODS : We performed a static mechanistic DDI prediction to evaluate whether previously reported competitive inhibition of
CYP3A by
telaprevir and its diastereomeric metabolite -
VRT-127394 is sufficient to explain the remarkable reduction in oral clearance observed with oral
midazolam and
tacrolimus . -
pubmed
In comparing
HLM incubations with and without a prior 30 - min exposure to
telaprevir , a respective 4- and 11 - fold reduction in IC50 was observed with
midazolam and
tacrolimus as substrates . -
pubmed
The
sst2 somatostatin receptor , targeted by
octreotide , is highly expressed in
meningiomas . -
pubmed
To investigate new therapeutic strategies , we evaluated the activity of
everolimus (
mTOR inhibitor ) ,
BKM-120 and
BEZ-235 ( new
Pi3K /
Akt /
mTOR inhibitors ) ,
octreotide and a combined treatment (
octreotide plus
everolimus ) , on cell proliferation , signaling pathways , and cell cycle proteins , respectively . -
pubmed
Further ,
everolimus decreased cell viability of
human meningiomas , but concomitantly , induced
Akt activation , reducing the antiproliferative effect of the drug . -
pubmed
The new
Pi3 K inhibitors were not more active than
everolimus alone , limiting their clinical relevance . -
pubmed
In contrast , a clear cooperative inhibitory effect of
octreotide and
everolimus was observed on cell proliferation in all tested
meningiomas , including WHO grades
II - III . -
pubmed
Octreotide not only reversed
everolimus - induced
Akt phosphorylation but also displayed additive and complementary effects with
everolimus on downstream proteins involved in translation (
4EB - P1 ) , and controlling cell cycle (
p27Kip1 and
cyclin D1 ) . -
pubmed
RESULTS :
Body weight , visceral fat ,
BMI and food intake decreased significantly in OVX +
AT +
Vit D ( P & lt ; 0.001 ) ; whereas these variables increased significantly in OVX + C ( P & lt ; 0.001 ) and SHAM ( P & lt ; 0.023 ) groups . -
pubmed
At the end of two - months of follow - up , we observed significant differences in
TC ,
TG ,
HDL - C ,
LDL - C ,
glucose ,
insulin , and HOMA - IR in all groups . -
pubmed
Total
glutathione (
GSH +
GSSG or
GSx ) ,
reduced ascorbate (
AH2 ) ,
uric acid ( UA ) and antioxidant enzymes were determined in lung , heart and BALF immediately ( 0 h ) or 20 - h
post 4 - h nose - only exposure to 0.0 , 0.25 , 0.5 and 1.0 ppm
O3 . -
pubmed
Two
CVD strains ( JCR and SHHF ) had high basal levels of
AH2 and
GSx in BALF as well as high basal lung UA . -
pubmed
Across all
strains , high BALF
GSx was only observed when high BALF
AH2 was present . -
pubmed
The results showed that animals fed with whole grain diets have lower food intake in comparison with the C diet , and decreased cecal content pH ( 7.06 vs. 6.33 ) and
beta - glucosidase activity ( 1.66 vs. 0.21 mumol
p - nitrophenol g(-1 ) cc h(-1 ) ) . -
pubmed
Our group synthesized
propane-2 - sulfonic acid octadec-9 - enyl - amide ( N15 ) , a novel
peroxisome proliferator activated receptor alpha (
PPARalpha ) agonist . -
pubmed
Simultaneous assessment of endogenous
thiol compounds by LC -
MS /
MS . -
pubmed
We reported a chemical derivatization- and LC -
MS /
MS - based approach to simultaneously determine
thiol compounds including
glutathione (
GSH ) ,
cysteine (
Cys ) ,
N - acetyl cysteine (
NAC ) ,
homocysteine (
Hcy ) , and
cysteinylglycine (
CysGly ) in biological samples . -
pubmed
Thiol - containing samples were derivatized with
monobromobimane (
mBrB ) at room temperature , followed by LC -
MS /
MS analysis . -
pubmed
PKA activation with a
cAMP analogue alleviated the
tumor vascular hyperpermeability , suggesting that the low
PKA activity in the endothelial cells may be responsible for the
tumor - tissue hyperpermeability . -
pubmed
Motesanib , a
kinase inhibitor for
VEGF receptor , activated
tumor endothelial
PKA and reduced the vascular permeability in the
tumor . -
pubmed
Different measures to evaluate the efficacy of
topiramate included subjective and objective assessment , photographs , the Skin Picking Scale modified after the Yale - Brown Obsessive - Compulsive Scale (
SPS - Y - BOCS ) , the Skin Picking Impact Scale , the Clinical Global Impressions - Improvement ( CGI - I ) and CGI - Severity scales , and the Beck Anxiety Inventory and Beck Depression Inventory . -
pubmed
The samples were extracted by a simple
protein precipitation method with
methanol . -
pubmed
The
MS analysis was performed with multiple reaction monitoring ( MRM ) , and the transitions were set at m / z 753.3 427.0 for
coumaroylspinosin and m / z 415.3 295.3 for IS , respectively . -
pubmed
Here we show that the
SETD8 /
PR -
Set7 methyltransferase , which catalyzes mono - methylation of histone
H4 at
lysine 20 ( H4K20me1 ) , suppresses nucleolar and mitochondrial activities to prevent cellular senescence . -
pubmed
Upon anchoring the
Ag NPs ,
ZnO NRs show enhanced band edge emission ( 380 - 400 nm ) and the emission is further significantly increased in
Ag NP - anchored
ZnO NTs . -
pubmed
It is believed that the near - field coupling of
SPR causes an optical enhancement of
ZnO , whereas the bridging effect and hot - electron transfer to the conduction band of
ZnO by plasmonic
Ag NPs , anchored in close proximity , gives rise to a faster response of the photodetectors . -
pubmed
RESULTS : In Experiment 1 ,
CBD alone produced no antinociceptive effects , while enhancing
THC - induced paw pressure but not tail withdrawal antinociception 4 - 6h
post -
THC injection . -
pubmed
CBD alone increased locomotor activity at 6h
post - injection , but enhanced
THC - induced
hypolocomotion 4 - 6h
post -
THC injection , at lower
THC doses . -
pubmed
We summarize here how
NPD1 elicits neuroprotection by up - regulating
c - REL , a
nuclear factor ( NF )- kappaB subtype that , in turn , enhances expression of
BIRC3 (
baculoviral inhibitor of apoptosis repeat - containing protein 3 ) in the retina and in experimental
stroke , leading to neuroprotection . -
pubmed
The molecular mass of the purified
TmrA was determined to be 44.5 kDa by
SDS - PAGE and MALDI -
TOF /
TOF . -
pubmed
The purified
dehalogenase reductively dechlorinated CF to
dichloromethane in vitro with reduced
methyl viologen as the electron donor at a specific activity of ( 1.27 + - 0.04 ) x 103 units mg protein-1 . -
pubmed
The most promising bioactive compound obtained from
TW is
celastrol . -
pubmed
The beneficial effects of
GIP were abrogated by inhibiting
nitric oxide ( NO ) synthase , suggesting a possible mechanism mediated by
NO . -
pubmed
Furthermore ,
GIP dose - dependently increased
NO production , whereas this effect was abolished by inhibiting
AMP - activated protein kinase (
AMPK ) . -
pubmed
GIP induced
AMPK phosphorylation , which was abrogated by inhibiting
phospholipase C and
calcium - calmodulin - dependent protein kinase kinase but not by
adenylate cyclase or
liver kinase B1 , suggesting the existence of a
calcium - mediated
GIPR signaling pathway . -
pubmed
Overall , we demonstrated the protective effects of
GIP against peripheral arterial remodeling as well as the involvement of a
calcium - mediated
GIPR signaling pathway in vascular endothelial cells . -
pubmed
The main factor of
ascites formation is renal
sodium retention due to activation of the
renin -
angiotensin -
aldosterone system and sympathetic nervous system by the reduced effective volume secondary to splanchnic arterial vasodilation . -
pubmed
The
enzyme catalysis of
AmyP and its mutants ( Q306A and E347A ) suggested that
calcium ions contributed to the hydrolysis of cyclic structures in raw microalgae starch by a distinctive
calcium - binding site
Ca2 of AmyP. -
pubmed
GlaxoSmithKline ( GSK ) has announced plans to buy Waltham , MA - based biotech Tesaro , maker of the once - daily oral
PARP inhibitor
niraparib ( Zejula ) , in a deal valued at $ 5.1 billion . -
pubmed
Benzo [ d ] thiazol-2 ( 3H )- ones as new potent selective
CB2 agonists with anti - inflammatory properties . -
pubmed
In this work , we report a new series of selective
CB2 agonists based on a
benzo [ d ] thiazol-2 ( 3H )- one scaffold . -
pubmed
Here , expression cloning with a cDNA expression library identified as an inhibitor of
HIV-1 infection , a
carboxy - terminally truncated form of
human POZ / BTB and
AT - hook - containing Zinc finger protein 1 (
PATZ1 ) , a transcriptional regulatory factor implicated in development and
cancer . -
pubmed
Experimental data of the
tubulin polymerization inhibition assay was validated by molecular docking technique and the results exhibited strong
hydrogen bonding interactions with
amino acids (
ASN-101 ,
TYR-224 ,
ASN-228 ,
LYS-254 ) of
tubulin . -
pubmed
RESULTS : In the training cohort ,
hepatocellular carcinoma (
HCC ) ,
diabetes , total
bilirubin (
TBIL ) ,
albumin (
ALB ) ,
alanine aminotransferase (
ALT ) , and serum
creatinine ( Scr ) were independent predictors of in - hospital
death . -
pubmed
In this work , the low - temperature
dehydration of
fructose to
HMF in different deep eutectic solvents (
DES ) was investigated . -
pubmed
We found a very active and selective reaction system consisting of the
DES tetraethyl ammonium chloride as
hydrogen bond acceptor (
HBA ) and
levulinic acid as
hydrogen bond donor (
HBD ) in a molar ratio of 1:2 leading to a maximum HMF yield of 68 % after 120 h at 323 K. The
DES still contained a low amount of water at the initial reaction , and water was also produced during the reaction . -
pubmed
However , the nature of the
HBA as well as the acidity of the
HBD play an important role for the maximum achievable HMF yield . -
pubmed
This was validated by measured yields in a
DES with different combinations of
HBD (
levulinic acid and
lactic acid ) and
HBA (
choline chloride and
tetra - n - alkyl ammonium chlorides ) . -
pubmed
In previous study ,
gallic acid (
GA ) , a part of
EGCG , has shown anti - allergic inflammatory effect . -
pubmed
Results : For in vitro ,
SG - SP1 reduced degranulation of mast cells by down - regulating intracellular
calcium levels in a concentration - dependent manner . -
pubmed
This inhibition was mediated by the reductions in serum
histamine and
interleukin-4 . -
pubmed
Here , the crucial role of the
ubiquitin lysine residue
K27 in the process of
histone H2A monoubiquitination mediated by the ubiquitin ligase
RNF168 has been showed . -
pubmed
The comparison of the turnover rate of the wild - type
ubiquitin and its variant with a single functional
lysine residue
K27 suggests an important role of the
ubiquitin deposition as a covalent conjugate with
histone H2A in terms of the stability of the entire ubiquitinome . -
pubmed
Nitric oxide production was measured by standard Griess reaction ,
arginase activity was determined by the measurement of
urea , reactive
oxygen species production was checked using
NBT dye reduction assay and electron paramagnetic resonance spectroscopy , cytotoxic activity was estimated in
MTT - assay . -
pubmed
Membrane
Cholesterol Regulates Endocytosis and Trafficking of the
Serotonin1A Receptor : Insights from Acute
Cholesterol Depletion . -
pubmed
Our results show that the
serotonin1A receptor exhibits agonist - induced
clathrin - mediated endocytosis with a concentration - dependent inhibition in internalization with increasing concentrations of
MbetaCD , which was restored upon
cholesterol replenishment . -
pubmed
Interestingly , subsequent to internalization under these conditions ,
serotonin1A receptors were re - routed toward lysosomal degradation , instead of endosomal recycling observed under normal conditions , thereby implicating membrane
cholesterol in modulation of intracellular trafficking of the receptor . -
pubmed
This raises the possibility of a novel
cholesterol - dependent role of intracellular sorting proteins in
GPCR trafficking . -
pubmed
These results differ from our previous observations on the endocytosis of the
serotonin1A receptor upon
statin - induced chronic
cholesterol depletion , in terms of endocytic pathway . -
pubmed
We conclude that analysis of complex cellular trafficking events such as
GPCR endocytosis under acute and chronic
cholesterol depletion conditions should be carried out with caution due to fundamental differences underlying these processes . -
pubmed
Full activity of thirteen
A3 - corroles is achieved with four
fluorine atoms present on the
meso - phenyl ring reaching a selectivity index above 300 . -
pubmed
To determine whether
cholesterol side - chain cleavage enzyme (
CSCCE ) was deficient or absent in tubules , mitochondria from tubules , interstitium and whole testes were analyzed for
CSCCE activity by measuring conversion of
cholesterol - 26 - 14C to
14C - isocaproate ( +
pregnenolone ) . -
pubmed
The enzyme shares 85 %
amino acid sequence identity with
Fuc - TIII and 89 % identity with
Fuc - TV but differs substantially in its acceptor substrate requirements . -
pubmed
This response was abolished following pharmacological blockade of
nicotinic receptors with
hexamethonium and
muscarinic receptors with
atropine . -
pubmed
A minimum of 6 weeks of co - treatment with
TPA and
rMuIFN - gamma ( zero time ) were necessary for demonstration of
rMuIFN - gamma - dependent co - promotion . -
pubmed
However , both doses of
DFMO completely suppressed
rMuIFN - gamma - dependent co - promotion . -
pubmed
The simplest explanation for the increased sensitivity to restriction enzymes in the mutant is that the biochemical defect in
XR-1 is not confined to the repair of ionizing radiation induced ends , but extends to DSBs that possess ligatable
3 '- hydroxyl and
5 '- phosphate ends as well . -
pubmed
The rotation of
cytochrome P-450 LM2 (
CYPIIB4 ) incorporated into large microsomal - like lipid vesicles was investigated by saturation transfer
EPR using
15N- and
2H - substituted spin labels . -
pubmed
32P -
Post - labeling analysis allowed reproducible separation of
DBF - DNA adducts and showed in addition the existence of several new adducts models of
DBF . -
pubmed
The allele - specific regulation of
C4 expression and the
androgen regulation of
Slp expression are manifest only in some of the tissues where these genes are expressed . -
pubmed
We show that these genes only affect the
Slp expression in tissues where it is
androgen - dependent . -
pubmed
This indicates that the non - H-2 regulatory genes most likely act through interaction with the
androgen regulation of
Slp expression . -
pubmed
The findings were : At 19 months of age CM showed higher
testosterone and
cortisol titers , higher
adrenal tyrosine hydroxylase activities and higher seminal vesicles weights than IM . -
pubmed
There is no apparent specificity for which strand of a duplex is initially cleaved by
EcoB , nor is there specificity with respect to the composition of the 3'-terminal
nucleotide formed on the DNA or the 3'- or 5'-terminal
nucleotides of the acid - soluble
oligonucleotides released during DNA cleavage . -
pubmed
LDL -
cholesterol ,
VLDL -
cholesterol , and
triglycerides during treatment periods with
pindolol or
atenolol , although there was a tendency to higher
triglyceride levels during
atenolol treatment . -
pubmed
The serum
HDL - cholesterol levels were significantly lower after 6 months of therapy with
atenolol than before treatment , but
HDL -
cholesterol levels increased slightly during
pindolol treatment . -
pubmed
The ratio of
HDL - cholesterol to total
cholesterol decreased significantly during 6 months of treatment with
atenolol . -
pubmed
Serum
insulin levels during oral
glucose tolerance test at 120 min were decreased after
pindolol therapy , but no significant changes were found in
C - peptide levels during treatment periods . -
pubmed
The immune response to a protein
antigen is mainly in the
IgG1 subclass , on the contrary the response to
polysaccharide antigens is mainly
IgG2 . -
pubmed
Effect of
obesity on ambient plasma
glucose , free
fatty acid ,
insulin ,
growth hormone , and
glucagon concentrations . -
pubmed
These
amylases were expressed only when the organism was grown on
maltose or other
carbohydrates containing
maltose units . -
pubmed
Amylase synthesis was more severely repressed by
glucose than by
xylose . -
pubmed
Amylases were produced in both wild - type and mutant
strains when starch was added to cells growing on
xylose but not when starch was added to cells growing on
glucose . -
pubmed
In both wild - type and mutant
strains ,
glucoamylase and
pullulanase were produced at high levels in starch - limited chemostats but not in
glucose- or
xylose - limited chemostats . -
pubmed
Micro method for determination of blocked
NH 2 - terminal amino acids of protein : application to identification of
acetylserine of
phosphoglycerate kinase and
pyroglutamic acid of
glucose 6 - phosphate dehydrogenase . -
pubmed
It was found that adrenalectomy was followed by a decreased
glucose - induced
insulin secretion ,
glucose oxidation ,
calcium uptake ,
cAMP and
insulin content without any remarkable change observed at the ultrastructural level . -
pubmed
[
Prostaglandins and the
kallikrein - kinin systems in the normal pregnancy - puerperium cycle and in
hypertensive disease proper to pregnancy ] . -
pubmed
Protein binding
VPA ( equilibrium dialysis ) and
FFA levels were determined on each sample . -
pubmed
It was found that the
chymotrypsin binding site that is not modified by mild oxidation with
N - chlorosuccinimide ( Shechter et al . -
pubmed
Structure of a
mercaptan - thermolysin complex illustrates mode of inhibition of
zinc proteases by substrate - analogue
mercaptans . -
pubmed
The structure of the complex of
thermolysin and the inhibitor (
2 - benzyl-3 - mercaptopropanoyl )- L - alanylglycinamide has been determined by X - ray crystallography at a resolution of 1.9 A and refined to a crystallographic residual of 18.4 % . -
pubmed
The binding of this potent , specific inhibitor to
thermolysin ( Ki = 7.5 X 10(-7 ) M ) serves as a model for the inhibition of
zinc peptidases by substrate - analogue
mercaptans . -
pubmed
The study shows that the
mercaptan inhibitor binds to thermolysin with the
sulfur , presumably in the anionic form , tetrahedrally coordinated to the
zinc and displacing a water molecule bound to the native
enzyme . -
pubmed
This is the first direct determination of the mode of binding of a
mercaptan inhibitor to a
zinc peptidase and confirms the geometry of binding expected on general grounds [ Ondetti , M. A. , Condon , M. E. , Reid , J. , Sabo , E. F. , Cheung , H. S. , & amp ; Cushman , D. W. ( 1979 ) Biochemistry 18 , 1427 - 1430 ; Nishino , N. , & amp ; Powers , J. C. ( 1979 ) Biochemistry 18 , 4340 - 4347 ] and inferred from previous spectroscopic studies [ Holmquist , B. , & amp ; Vallee , B. L. ( 1979 ) Proc . -
pubmed
The inclusion in the growth medium of
avidin , which almost irreversibly binds with
biotin ( Kd , 10(-15 ) M ) , results in an increase in cellular
biotin content and
biotin enzyme activity over that seen when the cells are grown in a
biotin - depleted medium . -
pubmed
The addition of
avidin - bound
biotin to the growth medium led to a forty - fold increase in cellular
biotin when compared to the inclusion of an equivalent amount of free
biotin in the medium . -
pubmed
Purified
bovine factor H appeared to bind agarose - bonded heparin through its anion - binding domain and the binding was inhibited by the presence of free
heparin or dextran
sulfate . -
pubmed
Calyculin A , the potent inhibitor of
type 1 ( PP1 ) and type 2A ( PP2A ) phosphatases , has been employed in order to investigate the role of endogenously activated
PP1 /
PP2A in the signal - transduction pathway of platelet - activating - factor ( PAF)-stimulated platelets . -
pubmed
Calyculin A alone caused an increase in protein phosphorylation in unstimulated platelets , with the detection of a number of newly phosphorylated proteins , whereas in
PAF - stimulated platelets phosphorylation of the major substrates of
protein kinase C and
myosin light - chain kinase were no longer transient , but phosphorylation was sustained . -
pubmed
PP1 / PP2A appear to play a role in
Ca2 + homoeostasis , as inhibition of
PP1 /
PP2A caused an inhibition of
Ca2 + mobilization and
Ca2 + influx through the plasma membrane in
PAF - stimulated platelets . -
pubmed
The results are discussed in relation to the possible role of
protein kinase C in mediating the events leading to the effects observed with
calyculin A. -
pubmed
These include a 12 -
amino acid insertion in
translation elongation factor 1 alpha and three small gaps in
enolase . -
pubmed
IgM antibodies to
Gal ( beta 1 - 3 ) GalNac gangliosides appeared to have similar specificities since these were positively correlated and inhibitable by cross adsorption assays . -
pubmed
Frequent amplification of a
short chain dehydrogenase gene as part of circular and linear amplicons in
methotrexate resistant Leishmania . -
pubmed
The
ltdh gene was amplified in all the mutants with the transport mutation of the first type , but not in all the mutants with a more pronounced decrease in the steady - state accumulation of
MTX . -
pubmed
In addition to these results with
isoflurane , we found that 15 V stimulation and tail
clamp produced comparable minimum alveolar concentration values for
halothane and for
desflurane . -
pubmed
We have used selection in MPP+ to clone the cDNAs encoding two
vesicular monoamine transporters , the first members of this novel gene family that now also includes the vesicular transporter for
acetylcholine . -
pubmed
This study investigates the reliability of
glycated haemoglobin , measured by electroendosmosis or by affinity chromatography ,
fructosamine , and
albumin adjusted
fructosamine , as indices of glycaemic control in
Type 1 diabetes complicated by
chronic renal failure . -
pubmed
Calculation of an
albumin adjusted
fructosamine result failed to improve the correlation with blood
glucose concentrations . -
pubmed
Like the
Grb2 adaptor protein , which is known to couple
receptor tyrosine kinases to the
small GTPase Ras ,
Nck is presumed to bind to
tyrosine - phosphorylated proteins using its SH2 domain and to downstream effector proteins using its SH3 domain . -
pubmed
The
Pak family of
serine / threonine kinases are known to be activated by binding to the
GTP - bound form of
Cdc42 or
Rac1 , which are small GTPases of the
Rho family that are involved in regulating the organization of the
actin cytoskeleton . -
pubmed
CONCLUSION : These results demonstrate that localization of
Pak1 to the membrane in the absence of other signals is sufficient for its activation , and imply that the
Nck adaptor protein could function to link changes in
tyrosine phosphorylation of cellular proteins to the
Cdc42 /
Pak signaling pathway . -
pubmed
In addition , co - injection of
RPP13 - 1 and
FITC - dextrans established that
RPP13 - 1 can induce an increase in plasmodesmal size exclusion limit to a value greater than 9.4 but less than 20 kDa . -
pubmed
The
enzyme was highly
oxygen sensitive and contained a redox - active
flavin cofactor , but no
iron centres . -
pubmed
Analysis of the derived
amino acid sequences revealed that the large subunit of the enzyme shares homology to
glycyl radical enzymes , particularly near the predicted radical site . -
pubmed
The radical - containing subunit of
benzylsuccinate synthase is oxygenolytically cleaved at the site of the
glycyl radical , producing the alpha'-subunit . -
pubmed
The four genes for
benzylsuccinate synthase and the activating enzyme are organized as a single operon ; their transcription is induced by
toluene . -
pubmed
Furthermore ,
NO levels were negatively correlated with the
EC - SOD levels in common EC -
SOD phenotype subjects . -
pubmed
One
amino acid substitution in the extracellular domain common to all known
leptin receptor proteins results from this mutation . -
pubmed
In each case ,
CGH successfully identified amplifications and/or deletions unique to the
drug - resistant selected cell lines . -
pubmed
The receptor - binding site is located in a C - terminal
disulphide - bonded loop of each
pilin monomer , but functional binding sites are displayed only at the tip of the pilus . -
pubmed
The pharmacy - based supervised self - administration of
methadone scheme (
SSA ) at the Camden and Islington Community Health Services Trust Substance Misuse Services ( CTDS ) in London has been found to be valuable by clients , pharmacists and keyworkers . -
pubmed
CONCLUSIONS : Despite the lower ambient
oxygen at high altitude and an increase in umbilical artery
PI , the fetal circulation does not exhibit a ' brain sparing effect ' . -
pubmed
Curcumin also decreases ERE (
estrogen responsive element )- CAT activities induced by
17 - beta estradiol . -
pubmed
Under optimal conditions ( 0.1 M K+-
Hepes , pH 7.3 )
GspS has
synthetase activity with apparent K(m ) values for
GSH ,
spermidine and
MgATP of 242 , 59 and 114 microM respectively , and a k(cat ) of 15.5 s(-1 ) . -
pubmed
Glutathionylspermidine is formed as end product and the
enzyme lacks
TryS activity . -
pubmed
coli
GspS , the recombinant enzyme also possesses
amidase activity (
EC 3.5.1.78 ) , hydrolysing
glutathionylspermidine to
GSH and
spermidine with a k(cat ) of 0.38 s(-1 ) and a K(m ) of 500 microM. -
pubmed
GspS can also hydrolyse
trypanothione at about 1.5 % of the rate with
glutathionylspermidine . -
pubmed
A single
amino acid mutation (
Cys-79 - ->
Ala ) is shown to ablate the
amidase activity without affecting the
synthetase activity . -
pubmed
Physiologic data provided evidence that
urea transport in red blood cells and kidney inner medulla was mediated by specific
urea transporter proteins . -
pubmed
One fragment , corresponding to residues 17 - 484 (
PhbN ' ) , contained the regulatory domain of the protein , but in missing the first 16 residues was devoid of the sole phosphorylation site of
Phb ,
Ser14 ; the second fragment corresponded to residues 485 - 843 (
PhbC ) and contained the catalytic domain of
Phb . -
pubmed
Our results support the idea that
amino acid residues proximal to the convertible
serine of
Phb are important for its specific interaction with the catalytic subunit of
PhK , but that regions distinct from the convertible
serine residue of
Phb and from the catalytic domain of
PhK may also be involved in the interaction of these two proteins . -
pubmed
[ Behavior of various
acetylenic quaternary ammonium salts with regard to serum and erythrocyte
cholinesterase levels ] . -
pubmed
[ ON THE ANTI - ARRHYTHMIC ACTION OF THE
MAGNESIUM DISODIUM SALT
AT EDTA ] . -
pubmed
First , we tested the hypothesis that endotoxin blocks the
estradiol - induced
LH surge centrally , by preventing the
GnRH surge . -
pubmed
Given this evidence that endotoxin blocks the
estradiol - induced
LH surge at the hypothalamic level , we began to assess underlying central mechanisms . -
pubmed
Although
flurbiprofen abolished endotoxin - induced
fever , which is a centrally generated ,
prostaglandin - mediated response , it failed to reverse blockade of the
LH surge . -
pubmed
Collectively , these results indicate endotoxin blocks the
LH surge centrally , suppressing
GnRH secretion via a mechanism not requiring
prostaglandins . -
pubmed
The sorption of
phenol ,
m - nitrophenol ( m -
NP ) , and
o - cresol from water onto montmorillonite modified with
cetyltrimethylammonium bromide (
CTAB ) in a column was studied . -
pubmed
RESULTS :
MARS therapy achieved a remarkable improvement in clinical symptoms and physic signs , accompanied with a significant decrease in serum
bilirubin ,
ammonia ,
urea nitrogen , fragrant
amino acid , endotoxin ,
IL-6 , and
TNF - alpha levels ( 0.05 ) ; at the 72 hours
bilirubin rebounding analysis . -
pubmed
MARS treatments resulted in a significant decrease of
bilirubin rebounding level in comparison with what
PE did ( 0.01 ) , though the
bilirubin removal efficiency between two groups was not statistically significant . -
pubmed
We identified a degenerate
retinoic acid response element ( RARE ) between -186 and -163 in the promoter region of the
tbx2 gene . -
pubmed
Previous studies have shown significant changes in
dopamine and opioid receptors in the basal ganglia following administration of
cocaine . -
pubmed
Cocaine administration results in a significant increase in the number of
opioid receptors in
dopamine - enriched brain regions . -
pubmed
These data also suggest that the molecular mechanism responsible for the up - regulation of microOrs in the caudate and putamen following
cocaine exposure may depend , in part , on the co - existence of
D2rs and micro - Ors in these cells . -
pubmed
Four polymorphisms were evaluated , including the
serotonin transporter
HTTLPR promoter ins / del ,
GABAAalpha6 Pro385Ser ,
NPY Leu7Pro , and
catalase 262C>T. Two of these , HTTLPR and
GABAAalpha6 Pro385Ser , had been previously associated with
LR and
alcoholism in a preliminary study . -
pubmed
CONCLUSIONS : This report strengthens the support for a relationship between the
HTTLPR L and
GABAAalpha6 Ser385 alleles to low
alcohol LR and to
alcoholism in a prospectively studied cohort evaluated for
LR in young adulthood and before the onset of
alcohol dependence . -
pubmed
Dihydro - S1P , which binds to and activates
S1P receptors but has no direct intracellular effect , suppressed neither apoptosis nor
NF - kappaB activity . -
pubmed
These results suggest that
S1P inhibits male germ cell apoptosis independently of its receptors , possibly by inhibiting the
transcription factor NF - kappaB and
Akt phosphorylation . -
pubmed
Several lines of evidence suggest that
D - serine , an endogenous agonist of the
glycine site on the
NMDA receptors , might play a role in the pathophysiology of
schizophrenia . -
pubmed
RESULTS :
Hydroxyproline levels for the three groups , F0 - 1 ,
F2 - 3 , and F4 , were significantly elevated as the
fibrosis score increased . -
pubmed
Several differences were noted between the amount of
collagen present according to
hydroxyproline measurement and the stages of
fibrosis according to histology . -
pubmed
CONCLUSIONS : Although increases in
collagen content ( by
hydroxyproline ) approximately paralleled increases in
fibrosis stage ( by histology ) , there was considerable overlap and some discrepancies . -
pubmed
A descriptive account is given of our most recent research on the
actinide dioxides with the Advanced Light Source Molecular Environmental Science (
ALS -
MES ) Beamline 11.0.2 soft X - ray scanning transmission X - ray microscope ( STXM ) at the Lawrence Berkeley National Laboratory ( LBNL ) . -
pubmed
This , along with the time course of
hemoglobin concentration and arterial
O ( 2 ) saturation ( infrared oximetry ) allowed computation of BBB
QaO ( 2 ) . -
pubmed
MATERIALS AND METHODS : The -I -
HDL kinetics were performed by infusion of
( 5.5.5 -( 2 ) H3 )- leucine for 14 h. The prebeta1 and
alphaHDL were separated by gel filtration fast protein liquid chromatrography system ( FPLC ) . -
pubmed
After inhibition with 5 mmol / L
H ( 2 ) O ( 2 ) to
FeSOD and
Cu /
ZnSOD ,
MnSOD enzyme activity was assayed with
nitrotetrazolium chloride blue staining . -
pubmed
This article focuses on the mechanisms by which hormone resistance develops , including the reactivation of
androgen receptor during
androgen deprivation therapy , the role of
cancer stem cells , and the emergence of epithelial - mesenchymal transition cells , which have increased metastatic potential . -
pubmed
The
ethylene - dependent
beta - xylosidase gene expression is hypothesized to relate to modification of cell walls in terminal buds during SD - induced growth cessation . -
pubmed
Effects of
Hydroxylamine on
Photosystem II : I. -
pubmed
Illumination of
chloroplasts in the presence of
NH ( 2 ) OH ( 2 mm ) leads to the destruction of all system II activities without affecting system I activity . -
pubmed
The system
II primary charge separation remains intact when incubated with this agent in the dark with release of one of the system
II Mn pools and simultaneous destruction of
O ( 2 ) evolving capacity . -
pubmed
Dekalb 202 ) was subjected to
O ( 2 ) stress ,
nodal roots with well developed cortical air spaces ( aerenchyma ) grew into the deoxygenated solution . -
pubmed
METHODS : Urothelium - denuded bladder neck strips were dissected and mounted in organ baths containing a physiological saline solution (
PSS ) at 37 degrees C and gassed with 5 %
CO ( 2 ) and 95 %
O ( 2 ) , for isometric force recording . -
pubmed
The relaxations to transmural nerve stimulation (
EFS ) or
PACAP 38 were performed on strips precontracted with 1 microM
phenylephrine (
PhE ) . -
pubmed
EFS experiments were carried out in the absence and the presence of
guanethidine ( 10 microM ) ,
atropine ( 0.1 microM ) , and
N ( G )- nitro - L - arginine (
L - NOARG , 100 microM ) , to block noradrenergic neurotransmission ,
muscarinic receptors , and
nitric oxide ( NO ) synthase , respectively . -
pubmed
RESULTS :
EFS ( 2 - 16 Hz , 1 ms duration , 20 sec trains , 75 mA current output ) evoked frequency - dependent relaxations which were reduced by the
VIP /
PACAP receptor antagonist
PACAP ( 6 - 38 ) ( 3 microM ) , and by the neurotoxin of the
capsaicin - sensitive primary afferents
capsaicin ( 10 microM ) , and abolished by the
neuronal voltage - activated Na (+ ) channel blocker
tetrodotoxin (
TTX , 1 microM ) . -
pubmed
The
vasoactive intestinal peptide ( VIP ) receptor antagonist [
Lys(1 ) ,
Pro(2,5 ) ,
Arg(3,4 ) ,
Tyr ( 6 )]- VIP ( 3 microM ) failed to modify the
EFS - induced relaxations . -
pubmed
PACAP 38 ( 1 nM-1 microM ) induced concentration - dependent relaxations which were reduced by
PACAP ( 6 - 38 ) ,
TTX and by the
neuronal voltage - gated Ca ( 2 + ) channel inhibitor omega - conotoxin GVIA (
omega - CgTX , 1 microM ) . -
pubmed
Fasting blood samples were analyzed for sex hormones , plasma
glucose ,
insulin ,
C - reactive protein (
CRP ) , and adipocytokines . -
pubmed
Insulin sensitivity ( assessed by homeostasis model assessment 2 modeling ) ,
androgen , and
HDL cholesterol levels were significantly decreased , whereas total fat mass and
LDL cholesterol ,
triglyceride ,
CRP ,
leptin , and
fructosamine levels were significantly increased in untreated
Klinefelter ' s syndrome patients . -
pubmed
In treated
Klinefelter ' s syndrome patients ,
LDL cholesterol and
adiponectin were significantly decreased , whereas no difference in body composition was found in comparison with untreated
Klinefelter ' s syndrome patients . -
pubmed
The proteins were adsorbed on
alkanethiol surfaces with different terminating groups and 50,000 best orientations of each
protein were analyzed . -
pubmed
Docking of the
tubulin dimer revealed that the orientation favored from the applicational point of view can be found in a list of the first 14 results for monolayers with different terminating groups and that there may be a noticeable difference in
tubulin dimer interactions with
alkanethiol chains of various length . -
pubmed
We further examined the effects of
PPARgamma agonist
15 - deoxy - Delta12,14 - prostaglandin J2 (
15d - PGJ2 ) , a naturally occurring
PPARgamma ligand , to these
endometrial carcinoma cell lines . -
pubmed
We also examined the status of apoptosis and
p21 mRNA expression of these
endometrial carcinoma cell lines following addition of
15d - PGJ2 . -
pubmed
We showed that
PPARgamma agonist
15d - PGJ2 inhibited cell proliferation and induced
p21 mRNA of
endometrial carcinoma cell lines . -
pubmed
These findings suggest that a
PPARgamma ligand ,
15d - PGJ2 , has antiproliferative activity against
endometrial carcinoma . -
pubmed
We found enhanced panicogenic sensitivity to
sodium lactate and an increased intensity and a differential pattern of Fos expression after the administration of
yohimbine or
caffeine in
TgNTRK3 . -
pubmed
We quantified four
monoesters , four oxidative
DEHP metabolites , and two secondary metabolites of
di - isononyl phthalate (
DiNP ) by a LC / LC -
MS /
MS method . -
pubmed
1 - O - alkylglycerols reduce the stimulating effects of
bFGF on endothelial cell proliferation in vitro . -
pubmed
Here we showed that
alkyl - Gro reduced the
basic Fibroblast Growth Factor (
bFGF)-stimulated endothelial cell proliferation in a concentration - dependent manner . -
pubmed
The effects of 0.5 and 5 ng / ml
bFGF on growth were completely suppressed after 72 h - treatment by 50 microM
alkyl - Gro . -
pubmed
Since
bFGF greatly increased ( +56%+/-15 ) the production of
1 - O - alkyl-2 - acyl - sn - glycerophosphate in
alkyl - Gro - treated endothelial cells , our data suggest that the observed effects of
alkyl - Gro could be mediated through
PLD activation . -
pubmed
Inhibition of
bFGF - stimulated endothelial proliferation could support anti - angiogenic activity of
alkyl - Gro . -
pubmed
In the present study ,
DS demonstrated a high level of binding activity to
receptor activator of NF - kappaB ligand (
RANKL ) and obstructed the binding of
RANK to
RANKL , determined using a
quartz - crystal microbalance ( QCM ) technique . -
pubmed
The
Fe - P ,
Al -
P and Oc - P have close relationships with the degree of pollution and may act as good indicators for environment pollution in Jiaozhou Bay . -
pubmed
Recruitment of the
p85 -
p110 complex to receptors and adaptor proteins via the
p85 SH2 (
Src homology 2 ) domains alleviates this inhibition , leading to
PI3 K activation and production of PIP(3 ) (
phosphatidylinositol 3,4,5 - trisphosphate ) . -
pubmed
Such a monomeric
p85 would compete with heterodimeric
p85 -
p110 for
pTyr (
phosphotyrosine ) recruitment , and thus repress
PI3 K activity . -
pubmed
Further , analysis of methylation status of proximal
GC boxes using
sodium bisulfite sequencing reveals differential methylation of proximal
GC boxes in the two cell lines examined . -
pubmed
Identification of a
chloride ion binding site in
Na +/ Cl - dependent transporters . -
pubmed
Here , we use calculations of pK(A)s and homology modeling to predict the location of a functionally important
Cl (- ) binding site in
serotonin transporter and other
Cl(-)-dependent transporters . -
pubmed
The process was studied by monitoring the change in
RB5 concentration and the intermediate products employing UV - visible spectrophotometry , ion chromatography ( IC ) , and gas chromatography/ mass spectrometry (
GC /
MS ) techniques and depletion in total organic
carbon ( TOC ) content as a function of irradiation time . -
pubmed
The optimal conditions for
DHCP generation were also established by autoclaving
GA ( pH 2.3 ) at 121 degrees C for 3h . -
pubmed
coli was restored when
superoxide dismutase and
catalase were added to the culture broth that contained
DHCP . -
pubmed
DATA SOURCES : Comprehensive search using keywords
AVP receptor antagonists ,
hyponatremia ,
SIADH ,
conivaptan ,
tolvaptan ,
lixivaptan , nurse practitioner , and others was carried out using the National Library of Medicine ( PubMed ) Web site from which full - text articles were obtained . -
pubmed
The inhibition of the proliferative burst in
MNU - treated parous mammary glands coincided with the upregulation of
Mnt , a
Myc - suppressor , and the formation of
histone deacetylase 1 /
Mnt /
Max complexes that unexpectedly contained
c - Myc . -
pubmed
These results suggest that the complexes also function as transcription repressors of the growth - related
Myc targets in
MNU - treated parous mammary glands . -
pubmed
BACKGROUND AND
AIM OF THE STUDY : Oral anticoagulation with
vitamin K antagonists is effective for the prevention and treatment of
thromboembolic events . -
pubmed
The soil - applied treatments (
paclobutrazol and
imidacloprid ) were applied once during the trial ( spring 2004 ) , and the foliarly applied treatments ( JA and harpin ) were applied each
spring . -
pubmed
In the present study , we examined the relevance of the apoptosis repressor with
caspase recruitment domain (
ARC ) on cardiac myocyte survival and its underlying mechanisms in a model of
Dox - induced
cardiotoxicity . -
pubmed
Proteasomal inhibitors partially rescued both
Dox - induced downregulation of
ARC protein and induction of apoptosis . -
pubmed
Knockdown of endogenous
ARC sensitised cardiomyocytes to undergo apoptosis upon treatment with
Dox . -
pubmed
In response to
Dox ,
Bax translocated from cytosol to mitochondria where it resulted in dissipation of the mitochondrial membrane potential ,
cytochrome c release and activation of
caspases - 3 and - 9 . -
pubmed
In this study , we provide evidence for the protective role of anti - apoptotic
ARC in
Dox - induced
cardiotoxicity , which makes this molecule an interesting target for future therapies . -
pubmed
In all groups anesthesia was induced with
sevoflurane , associated or not to
fentanyl , according to the following regimens :
G1 =
sevoflurane plus 2.5 microg.kg-1
fentanyl ; G2 =
sevoflurane plus 5 microg.kg-1
fentanyl ;
G3 =
sevoflurane plus 7.5 microg.kg-1
fentanyl ; and
G4 =
sevoflurane plus saline solution . -
pubmed
The following parameters were monitored :
SBP ,
DBP , HR , BIS ,
SE , RE ,
sevoflurane expired concentration ( EC ) and motor response to TI at three moments :
M1 = immediately before induction ; M2 = immediately before tracheal intubation and M3 = one minute after tracheal intubation . -
pubmed
In the present study , high - resolution solution NMR spectra were obtained of
OmpX kept water - soluble by either
amphipol A8 - 35 or the detergent
dihexanoylphosphatidylcholine . -
pubmed
During the first 6h
post - dose , the bronchodilatory effect of
aclidinium ( all doses ) on day 1 was comparable to that on day 29 . -
pubmed
Design , synthesis and preliminary activity assay of
1,2,3,4 - tetrahydroisoquinoline-3 - carboxylic acid derivatives as novel
Histone deacetylases (
HDACs ) inhibitors . -
pubmed
Herein , we report a novel series of
1,2,3,4 - tetrahydroisoquinoline-3 - carboxylic acid derivatives as
HDACs inhibitors . -
pubmed
Within this series , five compounds , 13a ( IC(50)=0.58+/-0.10 microM ) , 7d ( IC(50)=1.00+/-0.16 microM ) , 8l ( IC(50)=1.06+/-0.14 microM ) , 7i ( IC(50)=1.17+/-0.19 microM ) and 7a ( IC(50)=1.29+/-0.15 microM ) possessed better
HDACs inhibitory activity than
Vorinostat ( IC(50)=1.48+/-0.20 microM ) . -
pubmed
Core
2 beta1,6 - N - acetylglucosaminyltransferase (
C2GnT ) , which exists in three isoforms ,
C2GnT1 ,
C2GnT2 and
C2GnT3 , is one of the key enzymes in the
O - glycan biosynthetic pathway . -
pubmed
Functional analysis of two single
nucleotide polymorphisms in
SLC30A2 (
ZnT2 ): implications for mammary gland function and
breast disease in women . -
pubmed
Zinc transporter 2 (
ZnT2 ) plays a major role in
zinc (
Zn ) export from the mammary gland . -
pubmed
Recently , we determined that
ZnT2 is associated with secretory vesicles reflecting its role in
Zn secretion during lactation . -
pubmed
FluoZin-3 fluorescence illustrated increased lysosomal accumulation of
Zn in cells expressing
SNP1 concomitant with the abrogation of
Zn secretion . -
pubmed
In contrast , ectopic expression of
SNP2 was associated with the expansion of cytoplasmic
Zn pools , elevated reactive
oxygen species , and increased
Zn efflux . -
pubmed
Taken together , our data indicate that polymorphic variants in
ZnT2 distinctly alter mammary cell
Zn metabolism . -
pubmed
Several examined
hyperkalemia associated with
angiotensin - converting enzyme ( ACE )- inhibitor /
angiotensin II receptor blocker ( ARB ) , diuretic (
potassium - sparing ) , and beta - blocker use . -
pubmed
Finally ,
orthostatic hypotension was evaluated in those taking
ACE - inhibitor / ARB , beta - blocker , or
calcium - channel blocker therapy . -
pubmed
The roles of
p27 ( Kip1 ) ,
Bcl-2 , and
Cdk1 in apoptosis induced by
dasatinib and
paclitaxel were assessed using a terminal deoxynucleotidyl transferase - mediated
dUTP nick - end labeling ( TUNEL ) assay , siRNA knockdown of gene expression , transfection with
Bcl-2 and
Cdk1 expression vectors , and flow cytometry . -
pubmed
The siRNA knockdown of
Src ,
Fyn , or
Abl1 enhanced
paclitaxel - mediated growth inhibition in
ovarian cancer cells compared with a control siRNA . -
pubmed
The siRNA knockdown of
p27 ( Kip1 ) decreased
dasatinib- and
paclitaxel - induced apoptosis compared with a negative control siRNA ( sub - G1 fraction , control siRNA vs.
p27 ( Kip1 ) siRNA : 42.5 % vs. 20.1 % , difference = 22.4 % , 95 % CI = 20.1 % to 24.7 % , P = .017 ) . -
pubmed
Studies with forced expression and siRNA knockdown of
Bcl-2 and
Cdk1 suggest that
dasatinib - mediated induction of
p27 ( Kip1 ) enhanced
paclitaxel - induced apoptosis by negatively regulating
Bcl-2 and
Cdk1 expression . -
pubmed
CONCLUSION : Inhibition of
Src family and
Abl kinases with either siRNAs or
dasatinib enhances
paclitaxel sensitivity of
ovarian cancer cells through
p27(
Kip1)-mediated suppression of
Bcl-2 and
Cdk1 expression . -
pubmed
Metabolism and pharmacokinetic studies of
JPH203 , an
L - amino acid transporter 1 (
LAT1 ) selective compound . -
pubmed
Synthetic chemistry and in vitro screening efforts have afforded a variety of novel and highly
hLAT1 selective compounds , such as
JPH203 1 . -
pubmed
The current work develops a robust LC /
MS -
MS method to monitor 1 and its major Phase
II metabolite
N - acetyl - JPH203 2 from biological samples . -
pubmed
In 21 % of cases , analogous lesions involve the
CD58 gene , which encodes a
molecule involved in T and natural killer cell - mediated responses . -
pubmed
Neither
D1 - type (
SKF38393 ) nor
D2 - type (
quinpirole ) agonist was effective when given alone . -
pubmed
Twenty - four fully published articles were abstracted and summarised : 16 articles focused on five
peptide receptor radionuclide therapy (
( 111 ) In - DTPAOC ,
( 90 ) Y - DOTALAN ,
( 90 ) Y - DOTATOC ,
( 90 ) Y - DOTATATE , and
( 177 ) Lu - DOTATATE ) and eight focused on
( 131 ) I - MIBG treatment . -
pubmed
Thus , the capacity of endogenous free radical clearance can be of patho - physiological importance ; in this regard , the major reactive
oxygen species defense machinery , the
nuclear factor ( erythroid - derived 2 )- like 2 (
Nrf2 ) system needs to be precisely modulated in response to pathological alterations . -
pubmed
METHODS : CE -
MS analysis was performed on a 70 cm X 50 mum fused -
silica capillary . -
pubmed
The optimal
MS parameters were as follows : ESI -
MS analysis was performed in the positive mode ; 90 %
methanol containing 0.2 %
formic acid with a flow rate of 8 mul min(-1 ) was selected as the sheath liquid ; the flow rate and temperature of drying gas were 6 L min(-1 ) and 250 degree , respectively ; The nebulizing gas pressure was set at 5 psig ; The optimal values of fragmentor and ESI voltage were 100 V and 5 000 V , respectively . -
pubmed
Type
II natural killer T cells use features of both innate - like and conventional T cells to recognize
sulfatide self antigens . -
pubmed
Glycolipids presented by the
major histocompatibility complex ( MHC ) class I homolog
CD1d are recognized by natural killer T cells ( NKT cells ) characterized by either a semi - invariant T cell antigen receptor (
TCR ) repertoire ( type I NKT cells or iNKT cells ) or a relatively variable
TCR repertoire ( type
II NKT cells ) . -
pubmed
Here we describe the structure of a type
II NKT cell
TCR in complex with
CD1d -
lysosulfatide . -
pubmed
Both
TCR alpha - chains and
TCR beta - chains made contact with the
CD1d molecule with a diagonal footprint , typical of
MHC -
TCR interactions , whereas the
antigen was recognized exclusively with a single
TCR chain , similar to the iNKT cell
TCR . -
pubmed
Each chart was also reviewed for evidence of treatment with any
cholinesterase inhibitor ,
memantine , or both . -
pubmed
This study investigated optimizing the formulation parameters for encapsulation of a model mucinolytic enzyme ,
alpha - chymotrypsin ( alpha - CH ) , within a novel polymer ;
poly ( ethylene glycol )- co - poly ( glycerol adipate - co - omega - pentadecalactone ) ,
PEG - co -( PGA - co - PDL ) which were then applied to the formulation of
DNase I. alpha - CH or
DNase I loaded microparticles were prepared via spray drying from double emulsion ( w(1)/o / w(2 ) ) utilizing
chloroform (
CHF ) as the organic solvent ,
L - leucine as a dispersibility enhancer and an internal aqueous phase ( w(1 ) ) containing
PEG4500 or
Pluronic ( ) F-68 (
PLF68 ) . -
pubmed
alpha - CH released from microparticles was investigated for bioactivity using the
azocasein assay and the mucinolytic activity was assessed utilizing the degradation of
mucin suspension assay . -
pubmed
Optimum formulation
alpha - CH - CHF /
PLF68 , 1 % produced the highest bioactivity , enzyme encapsulation ( 20.08 + -3.91 % ) , loading ( 22.31 + -4.34 mug / mg ) ,
FPF ( fine particle fraction ) ( 37.63 + -0.97 % ) ; FPD ( fine particle dose ) ( 179.88 + -9.43 mug ) , MMAD ( mass median aerodynamic diameter ) ( 2.95 + -1.61 mum ) , and the mucinolytic activity was equal to the native non - encapsulated
enzyme up to 5h . -
pubmed
DNase I - CHF /
PLF68 , 1 % resulted in enzyme encapsulation ( 17.44 + -3.11 % ) , loading ( 19.31 + -3.27 mug / mg ) and activity ( 81.9 + -2.7 % ) . -
pubmed
Childhood
immune thrombocytopenia : role of
rituximab , recombinant
thrombopoietin , and other new therapeutics . -
pubmed
However , because immunosuppressive agents such as
rituximab are not approved for use in
ITP and the
thrombopoietin receptor agonists are not yet approved in children , the decision to use alternatives to splenectomy needs to be considered carefully . -
pubmed
Since externalization of
phosphatidylserine ( PS ) is one of the early molecular events during apoptosis ,
Annexin B1 (
AnxB1 ) , a member of Annexins family with high affinity toward the head group of PS , could be a potential positron emission tomography ( PET ) imaging probe for imaging cell
death process after labeled by positron - emitting nuclides , such as
( 18 ) F. -
pubmed
In the present study , we investigated a novel PET probe ,
( 18 ) F - labeled
Annexin B1 ( ( 18)F -
AnxB1 ) , for apoptosis imaging . -
pubmed
( 18 ) F - AnxB1 was prepared reliably by conjugating
AnxB1 with a
( 18 ) F - tag ,
N - succinimidyl 4 -[( 18 ) F ] fluorobenzoate (
[ ( 18 ) F ] SFB ) , in a radiolabeling yield of about 20 % within 40 min . -
pubmed
The in vitro binding of
( 18 ) F - AnxB1 with apoptotic cells induced by anti -
Fas antibody showed twofold increase compared to those without treatment , confirmed by flow cytometric analysis with AnxV -
FITC /
PI staining . -
pubmed
( 18 ) F - AnxB1 PET /
CT imaging was successfully applied to visualize in vivo apoptosis sites in
tumor induced by chemotherapy and in kidney simulated by
ischemia -
reperfusion injury . -
pubmed
As
TNF - alpha plays a pivotal role in the induction of early
atherosclerosis , treatment with
TNF - inhibitors may modulate
cholesterol handling , in particular ,
cholesterol efflux from macrophages . -
pubmed
Two of these genes (
FAM129A ,
SYNPO2 ) were associated with
airway hyperresponsiveness ( provocative concentration of
methacholine causing a 20 % drop in FEV1 : r = -0.740 , P & lt ; 0.01 ; r = -0.746 , P & lt ; 0.01 ) . -
pubmed
CONCLUSIONS : Oral
prednisolone changes the transcriptomic profile of the
ASM layer in
asthma . -
pubmed
Forward genetic studies have identified several
chloride (
Cl- ) channel genes , including
CFTR ,
ClC-2 ,
ClC-3 ,
CLCA ,
Bestrophin , and
Ano1 , in the heart . -
pubmed
A tetranuclear
Cu4 ( mu3 - OH ) 2 - based metal - organic framework (
MOF ) with
sulfonate -
carboxylate ligands for proton conduction . -
pubmed
A new tetranuclear
Cu4 ( mu3 - OH ) 2 - based metal - organic framework (
MOF ) with
sulfonate -
carboxylate ligands features large hydrophilic channels . -
pubmed
Nitric oxide in plants : the roles of
ascorbate and
hemoglobin . -
pubmed
Ascorbic acid and
hemoglobins have been linked to
nitric oxide metabolism in plants . -
pubmed
It has been hypothesized that
ascorbic acid directly reduces plant
hemoglobin in support of
NO scavenging , producing
nitrate and
monodehydroascorbate . -
pubmed
In this scenario ,
monodehydroascorbate reductase uses
NADH to reduce
monodehydroascorbate back to
ascorbate to sustain the cycle . -
pubmed
The product of the reaction is
monodehydroascorbate , which can be efficiently reduced back to
ascorbate in the presence of
monodehydroascorbate reductase and
NADH . -
pubmed
However , our
NO scavenging results suggest that the direct reduction of plant
hemoglobin by
ascorbic acid is unlikely to serve as a significant factor in
NO metabolism , even in the presence of
monodehydroascorbate reductase . -
pubmed
Investigation of the mechanisms underlying the high
acetylene - reducing activity exhibited by the soil bacterial community from
BC2 horizon in the permafrost zone of the East Siberian larch forest bed . -
pubmed
The calculated serum-
ascites gradients for the cardiac and malignant disease groups had a greater frequency of negative values signifying greater levels of
IL-8 ,
IL-10 and
MCP-1 in
ascites than did those with
alcohol or
HCV disease . -
pubmed
BACKGROUND :
Asymmetric dimethylarginine (
ADMA ) , the main endogenous inhibitor of
nitric oxide synthase , is considered to be associated with
endothelial dysfunction . -
pubmed
Dual - time - point
( 18 ) F - FDG PET imaging ( whole body ) was performed at 1 - h ( early )
post -
FDG injection and repeated ( 2 h delayed ) after injection . -
pubmed
The diagnostic accuracy of pre - PET staging and
post - PET staging was retrospectively evaluated , and the diagnostic accuracy of dual - time - point
( 18 ) F - FDG PET was compared to that of single - time - point
( 18 ) F - FDG PET . -
pubmed
Sugar esters are non - ionic surfactants that can be synthesized in a single enzymatic reaction step using
lipases . -
pubmed
Automated blood
glucose (
BG ) and
insulin pump systems allow wireless transmission of all
BG readings to a user 's pump . -
pubmed
5,7,3 ' , 4 '- Tetramethoxyflavone exhibits chondroprotective activity by targeting
beta - catenin signaling in vivo and in vitro . -
pubmed
In addition ,
PGE2 increased the expression of
cAMP /
PKA signaling pathway in chondrocytes , stabilized and accumulated
beta - catenin , and activated the expression of
beta - catenin signaling pathway . -
pubmed
Collectively ,
TMF is a potential compound with chondroprotective activity by inhibiting both
EP /
cAMP /
PKA signaling pathway and
beta - catenin signaling pathway . -
pubmed
However , modeling and experimental studies on the intravenous anesthetic
propofol indicate that potentiation of
gamma - aminobutyric acid receptors leads to a state of thalamocortical synchrony , observed as coherent frontal alpha oscillations , associated with
unconsciousness . -
pubmed
Sevoflurane , an
ether derivative , also potentiates
gamma - aminobutyric acid receptors . -
pubmed
The purpose of this study is to determine if there are differences in the expression of
estrogen - regulated genes ( ERGs ) , proliferation - associated genes and the
progesterone effector
RANKL , in premenopausal
ER + breast cancer as a result of the major changes in hormone levels that occur through the menstrual cycle . -
pubmed
Expression of
PGR ,
TFF1 ,
GREB1 and
PDZK1 followed the previously reported pattern : a higher level in W2 compared to W1 while W3 had an intermediate value , mirroring changes in plasma
estradiol . -
pubmed
Cation exchange - based
post - processing of
( 68 ) Ga - eluate : a comparison of three solvent systems for labelling of
DOTATOC ,
NO2AP(BP ) and
DATA(m . ) -
pubmed
In this work three cation exchange resin based
post - processing methods (
acetone ,
ethanol and
NaCl ) have been compared , using three model precursors (
DOTATOC ,
NO2AP(BP ) and
DATA ( m ) ) , in terms of labelling yield and reproducibility . -
pubmed
PURPOSE : Preliminary
iodine concentration ( UIC ) measurements in
spot urines of the representative German adult study
DEGS indicated a severe worsening of
iodine status compared to previous results in German children ( KiGGS study ) . -
pubmed
METHODS : UIC and
creatinine concentrations were measured in 6978
spot urines from the German nationwide
DEGS study ( 2008 - 2011 ) . -
pubmed
RESULTS : In
DEGS , median UIC was 69 microg / L in men and 54 microg / L in women , lying clearly below the WHO cutoff for
iodine sufficiency ( 100 microg / L ) . -
pubmed
Estimated median 24 - h UIE was 113 microg / day , accompanied by 32 % of
DEGS adults , lying below the estimated average requirement ( EAR ) for
iodine . -
pubmed
Reversible Oxidation of a Conserved
Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal
Nitrate Regulator
NirA. -
pubmed
In this work by mass spectrometry , we found that in the absence of
nitrate , when
NirA is inactive and predominantly cytosolic ,
methionine 169 in the nuclear export sequence (
NES ) is oxidized to
methionine sulfoxide (
Metox169 ) . -
pubmed
This oxidation depends on
FmoB , a
flavin - containing
monooxygenase which in vitro uses
methionine and
cysteine , but not
glutathione , as oxidation substrates . -
pubmed
nidulans cells to
nitrate led to rapid reduction of
NirA - Metox169 to
Met169 ; this reduction being independent from
thioredoxin and classical
methionine sulfoxide reductases . -
pubmed
Replacement of
Met169 by
isoleucine , a sterically similar but not oxidizable residue , led to partial loss of
NirA activity and insensitivity to
FmoB - mediated nuclear export . -
pubmed
Co - immunoprecipitation analysis of
NirA -
KapK interactions and subcellular localization studies of
NirA mutants lacking different parts of the protein provided evidence that
Met169 oxidation leads to a change in
NirA conformation . -
pubmed
Based on these results we propose that in the presence of
nitrate the activation domain is exposed , but the
NES is masked by a central portion of the protein ( termed
nitrate responsive domain , NiRD ) , thus restricting active
NirA molecules to the nucleus . -
pubmed
In the absence of
nitrate ,
Met169 in the
NES is oxidized by an
FmoB - dependent process leading to loss of protection by the
NiRD ,
NES exposure , and relocation of the inactive
NirA to the cytosol . -
pubmed
To avoid potential confounders , LTRs on oral hypoglycemic agents , exogenous
insulin ,
corticosteroids , or lipid - lowering therapy were excluded . -
pubmed
LTRs with
hepatic steatosis had higher serum concentrations of
small - dense low - density lipoprotein cholesterol (
sdLDL - C ; 34.8 + - 16.9 versus 22.7 + - 11.2 mg / dL ; P & lt ; 0.001 ) ,
sdLDL - C to
low - density lipoprotein cholesterol ratio ( 32.6 + - 11.6 versus 24.6 + - 10.2 ; P & lt ; 0.01 ) ,
small - dense low - density lipoprotein particle concentration (
sdLDL - P ; 770 + - 440 versus 486 + - 402 nmol / L ; P & lt ; 0.01 ) ,
very low density lipoprotein particle concentration (
VLDL - P ; 7.90 + - 7.91 versus 3.86 + - 3.18 nmol / L ; P & lt ; 0.01 ) , and
very low density lipoprotein size (
VLDL - size ; 51.9 + - 6.4 versus 48.7 + -
6.3 nm ; P = 0.06 ) . -
pubmed
LTRs with
hepatic steatosis had higher serum
insulin concentrations ( 27.8 + - 41.8 versus 11.7 + - 7.8 uU / mL ; P & lt ; 0.01 ) but similar fasting
glucose and
hemoglobin A1c . -
pubmed
An increased amount of
potassium significantly increases capacity , cycle life , and round -
trip efficiency . -
pubmed
The average effluent total
nitrogen concentration was below 5 mg L(-1 ) and
TN removal efficiency reached up to 97 % at
COD /
NO3 (- ) ratio of 3.0 under initial
NH4 (+ ) concentration of 25 mg L(-1 ) and
NO3 (- ) of 30 mg L(-1 ) . -
pubmed
At Cornell , no differences emerged on improvement on the primary outcome , estimated speed of change over time on the PDSS ; at Penn ,
ART ( P = .025 ) and
CBT ( P = .009 ) showed greater improvement at treatment termination than
PFPP . -
pubmed
Combined
PCD /
chlorine treatments resulted in greater disinfection efficiency than those for the two individual treatments . -
pubmed
Combination of 0.9 MPa
PCD ( various
CO2 supply rates : 25 %
CO2 + 75 %
N2 , 50 %
CO2 + 50 %
N2 , and 100 %
CO2 ) and
chlorine ( 0.20 mg L-1 ) yielded average synergy values of 4.9 , 5.2 , and 4.4 log , respectively , within 3 min . -
pubmed
This was deduced from the significant increase in
H2O2 level ,
superoxide dismutase and
catalase activities and
malondialdehyde content . -
pubmed
Maghemite (
gamma - Fe2O3 ) nanoparticles were obtained by coprecipitation of
ferrous and
ferric salts in an alkaline medium followed by oxidation ; the nanoparticles were coated with
poly ( N , N - dimethylacrylamide ) (
PDMA ) and characterized by transmission electron microscopy , attenuated total reflection (
ATR ) Fourier transform infrared ( FTIR ) spectroscopy , dynamic light scattering , thermogravimetric and elemental analyses , and magnetic measurements in terms of particle morphology , size , polydispersity , amount of coating , and magnetization , respectively . -
pubmed
Moreover , increased antitumor activity was observed after combined administration of
gamma - Fe2O3@
PDMA nanoparticles and
Toc-6 - Ac ; however , levels of
bilirubin ,
aspartate aminotransferase , and white bloods normalized and did not differ from those of the intact controls . -
pubmed
This effect was associated to a significant inhibition of
nitroglycerin - induced increase in c - Fos ,
TRPA1 and neuropeptides mRNA levels in medulla - pons area , in the cervical spinal cord and in the trigeminal
ganglion . -
pubmed
caroliniana were gradually compromised as time went by , and the down - regulations of the relative growth rate (
RGR ) and photosynthetic activity were coupled with phytoplankton proliferation under elevated atmospheric
CO2 . -
pubmed
L - mimosine induces
caspase-9 - mediated apoptosis in
human osteosarcoma cells . -
pubmed
The Hoechst staining results and
TEM revealed that nuclear damage increased with the concentration increase in
L - mimosine , as did the formation of apoptotic bodies . -
pubmed
Additionally , the results of the western blot analysis confirmed that the treatment of cells with
L - mimosine was accompanied by increasing expression of
cleaved caspase-9 . -
pubmed
L - mimosine - induced apoptosis was inhibited by the
caspase-9 inhibitor
Z - LEHD - FMK . -
pubmed
In addition , the
extracellular signal - regulated kinase (
ERK ) signaling pathway was suppressed following treatment with
L - mimosine . -
pubmed
A total of 34
phenolic compounds were identified and quantitatively monitored by UPLC - ESI - QTOF -
MS . -
pubmed
This undesired
triethanolamine deposit was easily removed from the surface by electrochemical voltage cycling or with an aqueous acidic
wash for 1 h. -
pubmed
Antiparasitic activity of new
gibbilimbol analogues and
SAR analysis through efficiency and statistical methods . -
pubmed
The conversion of
ent - CPP ,
ent - LPP ,
syn - CPP , and
ent - neo - CT - CLPP by promiscuous
diTPSs further yielded distinct
labdane - type
diterpene olefins and
alcohols . -
pubmed
Leptin increased carotid
sinus nerve activity at baseline and in response to
hypoxia in vivo . -
pubmed
We found that
leptin enhanced carotid
sinus nerve activity at baseline and in response to 10 %
O2 in vivo . -
pubmed
Furthermore , TP42/40 was inversely associated with neocortical
amyloid deposition ( measured with PiB - PET ) and was concordant with the
AD biomarker profile in cerebrospinal fluid (
CSF ) . -
pubmed
The results showed that 48 - h exposure to PS ( 0.1 mg / L ) or
ROX ( 0.01 mg / L ) alone activated the activities of
CAT and
GST and
MDA levels . -
pubmed
magna co - exposed to 1 - mum PS were significantly decreased , while co - exposure to 10 - mum PS significantly decreased the responses of
GST and
MDA . -
pubmed
Novel
Isoxazole Derivatives with Potent
FXR Agonistic Activity Prevent
Acetaminophen - Induced
Liver Injury . -
pubmed
In this Letter , we have elaborated on the structure of
GW4064 , the first nonsteroidal agonist for
FXR , to identify novel
isoxazoles endowed with
FXR agonistic activity and improved ADME properties . -
pubmed
While widely used fluorescent proteins , such as
green fluorescent protein ( GFP ) , have had huge impact in biological imaging because they provide genetically encoded , highly specific labeling , these probes require
oxygen to generate fluorescence . -
pubmed
Luteolin supplementation also significantly lowered mRNA expression of inflammatory and
M1 markers
MCP-1 ,
CD11c ,
TNF - alpha and
IL-6 , while maintaining expression of M2 marker
MGL1 . -
pubmed
pylori infection includes
clarithromycin triple therapy (
clarithromycin ,
proton pump inhibitor [ PPI ] , and
amoxicillin or
metronidazole ) ,
bismuth quadruple therapy (
bismuth salt ,
PPI ,
tetracycline , and
metronidazole or
amoxicillin ) , or concomitant therapy (
clarithromycin , PPI ,
amoxicillin , and
metronidazole ) . -
pubmed
On d11 , P and
NP were similar with low PGFM and no effect of
oxytocin on PGFM . -
pubmed
Alkynyl Benzoxazines and
Dihydroquinazolines as
Cysteine Targeting Covalent Warheads and Their Application in Identification of Selective Irreversible
Kinase Inhibitors . -
pubmed
A potent covalent inhibitor of
JAK3 kinase was identified with superior selectivity across the kinome and improvements in in vitro pharmacokinetic profile relative to the related
acrylamide - based inhibitor . -
pubmed
In addition , the use of a novel
heterocycle as
cysteine reactive warhead is employed to target
Cys788 in
c - KIT where
acrylamide has previously failed to form covalent interactions . -
pubmed
Univariate analysis and multivariate Cox regression modelling were used to identify factors pre- and
post -
TACE therapy significantly associated with survival . -
pubmed
On multivariate analysis greater
tumour number ( p = 0.02 ) , higher serum
bilirubin ( p = 0.007 )
post initial TACE , and
hepatic decompensation ( p = 0.001 )
post second TACE were associated with reduced survival . -
pubmed
On a per - lesion basis , the sensitivity of CEUS , MDCT , MRI , and
FDG PET /
CT was 86 % , 84 % , 89 % , and 62 % , respectively . -
pubmed
On a per - patient basis , the sensitivity and specificity of CEUS , MDCT , MRI , and
FDG PET /
CT was 80 % and 97 % , 87 % and 95 % , 87 % and 94 % , and 96 % and 97 % , respectively . -
pubmed
The sensitivity for MRI was higher than that for CEUS , MDCT , and
FDG PET /
CT for
lesions < 10 mm and
lesions at least 10 mm in size . -
pubmed
Melatonin is a hormone that produces behavioral , pharmacological , and physiological effects through the activation of
MT1 and
MT2 melatonin receptors . -
pubmed
Melatonin receptors participate in the modulation of the reinforcing effects of
cocaine . -
pubmed
Some studies report that dosing of
melatonin decreases
cocaine - induced locomotor activity and
cocaine self - administration and that
luzindole , an
MT1 , and
MT2 melatonin receptor antagonist , blocks the
melatonin - dependent decrease in
cocaine - induced locomotor activity . -
pubmed
Melatonin decreased
cocaine - induced locomotor sensitization and intracellular
dopamine levels , as well as
cocaine -
CPP . -
pubmed
These data suggest that
melatonin may be a useful therapeutic agent to reduce
cocaine abuse ; additionally , they suggest that
MT1 and
MT2 receptors could be therapeutic targets , useful for the treatment of
drug abuse disorder . -
pubmed
Besides , we isolated the promoter / enhancer of
Ds serendipity alpha to drive the heterologous
tetracycline - controlled transactivator ( tTA ) during early embryonic development and generated a testes - specific tTA driver line using the endogenous
beta-2 - tubulin (
beta2 t ) promoter / enhancer . -
pubmed
The experiment was tested on uniformly
15N- and
13C - enriched T4
lysozyme . -
pubmed
The ability to react with
fibronectin of the antigens of
58 - 60 and 30 - 31 kD was demonstrated by parallel labelling with a
fibronectin -
biotin conjugate . -
pubmed
There currently exist various opinions concerning the best therapy for managing
hydrogen fluoride (
HF )
dermal burns . -
pubmed
Whole cell voltage -
clamp recordings of the synaptic currents corresponding to these synaptic potentials in the presence of pharmacological antagonists of the neurotransmitters
gamma - aminobutyric acid (
GABA ) ,
glutamate , and
glycine receptor subtypes indicate that the inhibitory synaptic currents are mediated by
GABA - activated Cl- channels , while the excitatory synaptic currents are due to activation of
ionotropic glutamate receptors of the
N - methyl - D - aspartic acid (
NMDA ) and non -
NMDA subtypes . -
pubmed
Phase I -
II trial of hyperthermic isolated limb perfusion with
cisplatin in the treatment of high risk
malignant melanoma of the extremities . -
pubmed
Concomitantly , activation of right atrial
adenylate cyclase by 10 microM
GTP , 10 microM
isoprenaline and 1 microM
forskolin was enhanced and inhibition by 100 microM
carbachol was diminished . -
pubmed
On isolated , electrically driven right atria the
beta 1 - adrenoceptor - mediated positive inotropic effect of
noradrenaline was - even with
beta 1 - adrenoceptor number increased - not altered , while the
beta 2 - adrenoceptor - mediated effect of
procaterol was markedly enhanced . -
pubmed
The effect of T4 non - carrying
thyroxine binding globulin (
TBG ) on free
thyroxine determination using the microencapsulated
antibody method was studied , to investigate the precise reliability of the membrane and to find possible applications for estimating other free
steroid hormones . -
pubmed
All ( 6/6 )
FP / FL recipients were cured (
glucose less than 250 mg / dl for greater than 30 days ) , whereas only 72 % ( 13/18 ) of
FP alone and 60 % ( 3/5 ) of
FP /
FS recipients were cured . -
pubmed
In conclusion , FL in a composite graft with
FP resulted in better engraftment , earlier isograft function , and protection from
cyclosporine islet toxicity . -
pubmed
When we treated the monolayers with drugs commonly used for the treatment of
inflammatory bowel disease , including
prednisolone ,
5 - aminosalicylic acid ,
metronidazole , and
6 - mercaptopurine , we were not able to demonstrate a reduction in carrageenan - induced cell
injury ; however ,
catalase at 1 mg / ml decreased carrageenan
cytotoxicity . -
pubmed
Phencyclidine binds with high affinity to both
PCP and
sigma receptors . -
pubmed
Among the thirty - three
nitro compounds other than the
nitrofuran derivatives tested as substrates , the purified
enzyme showed activity only toward
o - dinitrobenzene ,
4 - nitroquinoline N - oxide ,
3,4 - dinitrobenzoic acid ,
p - dinitrobenzene ,
2,5 - dinitrobenzoic acid ,
2,5 - dinitrophenol ,
tetra - chloronitrobenzene and
2,4 - dinitrobenzoic acid . -
pubmed
Among the other nine
2 - substituted 5 - nitrofuran derivatives tested , six compounds could be the substrates of the
enzyme , and
5 - nitrofurfural and
5 - nitrofurfural diacetal were especially good substrates . -
pubmed
The bulky substituents at position 2 appeared to decrease the susceptibility of these
nitrofuran derivatives to the
enzyme . -
pubmed
In a short term study with 200 micrograms
SMS bid , symptoms of
hypercorticism disappeared while
cortisol and
ACTH serum concentrations fell below the normal values . -
pubmed
Excellent clinical efficacy as well as normal
cortisol and
ACTH serum concentrations were maintained during the nine month follow up . -
pubmed
These findings suggest that the contractile response to
nicotine was mediated through an action on the
nicotinic receptors and partially due to the release of
acetylcholine and
tachykinins . -
pubmed
These findings suggest that chronic
nicotine treatment might cause a decrease of the amounts of
nicotinic receptors and also receptors for mediators released by
nicotine . -
pubmed
Chronic
haloperidol treatment resulted in a 3.5 fold increase in striatal
proenkephalin mRNA and a 30 % decrease in
protachykinin mRNA ( no apparent change in
alpha - tubulin mRNA was observed ) . -
pubmed
The
enzyme activity was stimulated 7 - fold in the presence of
cardiolipin or
phosphatidylglycerol ( and 3 - fold by
phosphatidylethanolamine ) but not other
phospholipids . -
pubmed
The kinetic analysis indicated that addition of
phosphatidylglycerol has a negligible effect on apparent Km values but increases the Vmax of the
enzyme 5- to 6 - fold . -
pubmed
The
enzyme activity was solubilized by the dialyzable detergent
CHAPSO without inhibition of the
enzyme activity , and the solubilized
enzyme in the presence of 0.4 %
CHAPSO is partially purified by chromatography on
GDP -
hexanolamine - Sepharose . -
pubmed
Removal of
CHAPSO from the affinity purified
enzyme by dialysis resulted in a 66 % loss of the original activity , which was restored by addition of
phosphatidylglycerol . -
pubmed
Chromatography of the affinity - purified
enzyme with
3H - labeled phosphatidylglycerol on a Biogel A0.5 column indicated an association of the
enzyme with the
phospholipid that occurred only in the absence of detergent . -
pubmed
These results suggest that
phospholipid has a direct effect on the
enzyme and that the inhibitory effect of detergents can be ascribable to disturbing interaction between
phospholipids and the
enzyme . -
pubmed
A possible role of specific
phospholipids on in vivo
transferase activity for
glycolipids is discussed . -
pubmed
The results show that plasma and intraerythrocyte
lactate concentrations were not significantly different from each other at
rest or moderate ( less than or equal to 50 % VO2max ) exercise . -
pubmed
The plasma to red cell
lactate gradient reached a mean ( + /-
SE ) 1.7 + /- 0.4 mmol . -
pubmed
Interestingly , at 15 - min
post - exercise the direction of the
lactate gradient was reversed , with the mean intraerythrocyte concentration now being significantly increased over that found in the plasma . -
pubmed
The best results were found in the males group , where
cholesterol HDL increased and
triglycerides and beta / alpha ratio decreased . -
pubmed
In the females the only good result was represented by the increase of
HDL -
cholesterol , while the other values did not change . -
pubmed
Chemical modification of
cytochrome P-450 LM2 with
N - acetylimidazole . -
pubmed
Cytochrome P-450 LM2 has been reacted with
N - acetylimidazole . -
pubmed
About three
tyrosyl residues of
cytochrome P-450 LM2 are accessible to O - acetylation . -
pubmed
The analysis of the spectral dissociation constants , substrate binding kinetics , reduction kinetics ,
N - demethylase activity and substrate conversion by artificial
oxygen - donating agents of differently acetylated
enzyme provides evidence for the existence of two groups of accessible
tyrosines . -
pubmed
One
tyrosyl residue is located in the immediate environment of the
heme iron and is involved in the binding of type
II substrates . -
pubmed
The results strongly suggest
tyrosine(s ) to be involved in
NADPH - cytochrome P-450 reductase dependent N - demethylation . -
pubmed
The
tubulin synthesized in vitro is characterized by ( i ) its electrophoretic mobility on
sodium dodecyl sulfate -
acrylamide gels ( brain : 55,000 daltons ; leg muscle : 53,000 daltons ) , and ( ii ) its ability to function as a microtubular subunit , as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro . -
pubmed
Mechanism of
urocanase as studied by
deuterium isotope effects and labeling patterns . -
pubmed
Most of the
protein which bound to R 1881 was recovered in the precipitate of a 0 - 30 % saturation of
ammonium sulfate , and was eluted in the void volume on a Sephadex G-200 column . -
pubmed
The
renin -
angiotensin -
aldosterone system during and after cardiopulmonary bypass . -
pubmed
A chromatographic system consisting of Sorbax -
SIL and Sorbax - CN columns , organic solvent extraction and a UV detector was used to analyze both more polar and less polar
corticosteroids in the adrenal tissue . -
pubmed
In addition , competition with the radioactively labeled translation product occurred only in the presence of purified
Vt , but not
BSA or
LPI . -
pubmed
The removal of
oligosaccharides from purified
Vt by enzymatic digestion with
N - glycosidase F resulted in the appearance of a smaller polypeptide of 36 kDa , which reacted immunologically with Vt antiserum in a Western blot . -
pubmed
After
6 - OHDA lesion , as well as caudally to the transection , a significant increase by 21 % ( P & lt ; 0.01 ) to 68 % ( P & lt ; 0.001 ) of
alpha 1 - adrenoceptors densities was detected . -
pubmed
The mechanism of action seems to be through decreasing intracellular
calcium by inhibiting its uptake and stimulating the efflux , and by an inhibition on
protein kinase C. -
pubmed
Extracellular
glutamate rose quickly after
vessel occlusion and peaked at 33.95 + /-
6.3 mumol / ml 30 minutes after
MCAO . -
pubmed
By 60 minutes after
MCAO , this level had decreased to 25.14 + /-
6.3 mumol / ml ;
glutamate levels decreased slightly to 21.35 + /- 6.8 mumol / ml by 120 minutes . -
pubmed
Long - term treatment with the
cholinesterase inhibitor
tacrine increase the uptake of
[ 11C ] nicotine . -
pubmed
Significant reduction in uptake between the two enantiomers ( S)(- ) and
( R )(+)-[ 11C ] nicotine has been observed compatible with a restoration of
nicotinic receptors . -
pubmed
A point mutation in the
bile acid biosynthetic enzyme
sterol 27 - hydroxylase in a family with
cerebrotendinous xanthomatosis . -
pubmed
An increase in plasma
cholestanol alone would , thus , not appear to be a direct cause of
sterol storage in
CTX , while
CTX is strongly suggested to be caused by defects in both alleles of the
CYP27 gene . -
pubmed
These results suggest that the fast inactivating ( transient ) current might be T - type
Ca2 + current [ ICa(T ) ] , and such cells were ICa(T ) positive cells ; whereas the sustained
Ca2 + current was L - type
Ca2 + current [ ICa(L ) ] , and such cells were
ICa ( L ) positive cells . -
pubmed
Kinetic and pH titration experiments reveal that when
His57 is deprotonated the
enzyme binds its substrates randomly . -
pubmed
However , when
His57 is protonated the
enzyme observes the obligatory substrate binding order as seen for the wild type . -
pubmed
A comparison of the reaction schemes and pH profiles of the wildtype and
His57 enzymes further indicates that protonation of residue 57 facilitates formation of the binary
enzyme -
carbamoyl phosphate complex and augments the turnover rate of the reaction . -
pubmed
265 , 2620 - 2623 ) that the turnover rate of the
wild - type ornithine transcarbamoylase in the forward reaction is largely dictated by the rate of the
carbamoyl phosphate - induced isomerization . -
pubmed
Highest levels of serum
cholesterol were found in
spring and lowest in summer . -
pubmed
These changes were measured using
MTT to assess
mitochondrial reductase activity. -
pubmed
Phosphatidylinositol-4 - phosphate 5 - kinases (
PIP5 K ) synthesize
phosphatidylinositol-4,5 - bisphosphate , a key precursor in
phosphoinositide signaling that also regulates some proteins and cellular processes directly . -
pubmed
Thus ,
CD22 negatively regulates antigen receptor signaling in the absence of
antigen . -
pubmed
Mechanisms of centrally administered
ET-1 - induced increases in systemic arterial pressure and
AVP secretion . -
pubmed
Endothelins (
ET ) within the central nervous system ( CNS ) alter systemic cardiovascular responses and
arginine vasopressin (
AVP ) secretion . -
pubmed
These experiments were designed to ascertain whether the rise in systemic arterial pressure after central administration of
ET-1 is mediated by enhancing sympathetic outflow and/or circulating
AVP . -
pubmed
Thus
ET-1 via
ETA receptors within the CNS induced a concentration - dependent increase in systemic arterial pressure mediated by enhanced sympathetic outflow but not by circulating
AVP . -
pubmed
While whole body
glucose removal and production may be reduced following training , this has only been demonstrated under exercising conditions in which glycemia demonstrates little deviation from
rest . -
pubmed
The
CR1 genotype was determined by a method based on
polymerase chain reaction ( PCR ) amplification of the genomic DNA fragment of 1.8 kb separated by
HindIII endonuclease digestion and
agarose gel electrophoresis . -
pubmed
Beta - lactam resistance in mycobacteria results from an interplay between the following : ( i )
beta - lactamase production , ( ii ) affinity of the
penicillin - binding proteins (
PBPs ) for the drugs , and ( iii ) permeation of the drugs . -
pubmed
However , the affinities of
PBP 1 for the
beta - lactams tested were lower for the mutant than for the wild type . -
pubmed
Taken together , the results suggest that decreased affinities of
PBP 1 for
beta - lactams , combined with the decreased permeability of the cell wall of the mutant , lead to the development of high - level acquired
beta - lactam resistance . -
pubmed
This effect , which was also observed with
amphotericin B - treated corneas , was not mediated by classical
beta - receptors based on the results obtained with
isoproterenol and
propranolol . -
pubmed
The similarities , of a sequence motif found in
potassium channels and
beta - adrenergic receptor kinases that are regulated by the
beta gamma subunit of G proteins with that found in
aquaporins 2 and 5 , could explain the link with
epinephrine . -
pubmed
or administered orally led to a marked loss of uterine and vaginal
estrogen receptor levels measured by binding assay , whereas
ICI 182 780 exerted no inhibitory effect on this parameter under the experimental conditions used . -
pubmed
Similarly , because
P450s are catalyzing the toxication and detoxication of
environmental pollutants , an understanding of these reactions facilitates risk assessment in environmental toxicology . -
pubmed
RESULTS : Using multiple linear regression analysis , total
calcium scores were better independent predictors of both CAGE & gt ; or = 20 and CAGE & gt ; or = 50 scores than either a SPECT - derived radionuclide perfusion score or the risk factors age , male gender and ratio of total /
high - density lipoprotein (
HDL )
cholesterol . -
pubmed
Biochemical analyses revealed that
Abeta purified from
senile plaques and vascular amyloid are isomerized at
Asp-1 and
Asp-7 . -
pubmed
A specific antibody recognizing isoAsp-23 of
Abeta further suggested the isomerization of
Abeta at
Asp-23 in vascular amyloid as well as in the core of
senile plaques . -
pubmed
Biochemical analyses of purified PHFs also revealed that heterogeneous molecular weight
tau contains
L - isoaspartate at
Asp-193 ,
Asn-381 , and
Asp-387 , indicating a modification , other than phosphorylation , that differentiates between normal
tau and
PHF tau . -
pubmed
Protein L - isoaspartyl methyltransferase (
PIMT ) is suggested to play a role in the repair of isomerized proteins containing
L - isoaspartate . -
pubmed
By multivariate analysis , the
DH ( + ) and DH ( - ) groups of
NTG showed significantly longer peak times and tau s than did the non - glaucomatous eyes ( p & lt ; 0.05 ) . -
pubmed
However , no statistically significant differences were observed in any parameters between the
DH ( + ) and DH ( - ) groups of
NTG . -
pubmed
A new covalent modification of
p53 at
lysine 386 by
SUMO-1 was recently identified . -
pubmed
These data indicate that
SUMO-1 modification of
p53 at
lysine 386 may not be essential for
p53 's cellular localization , transcriptional activation , or growth regulation . -
pubmed
In the C480Y mutant ,
tyrosine 480 protrudes from
S6 to near
S5 , suggesting that the bulky
side chain may provide steric hindrance to the rotation of
S6 that has been proposed to accompany the open - closed state transitions ( Perozo , E. , D.M. -
pubmed
The
side chain at position 480 in
S6 in the pore model is close to and may interact with a conserved
glycine ( G421 ) in
S5 . -
pubmed
Replacement of
G421 with bulky side chains also leads to channels that are trapped in an active state , suggesting that
S5 and
S6 interact with each other during voltage - dependent open - closed state transitions , and that bulky side chains prevent the dynamic changes necessary for permanent channel closing . -
pubmed
The specific activity of
lipoxygenase from several
strains of the zygomycete Mortierella varied from 1.02 to 2.02 microMol
diene per min per mg
protein ) . -
pubmed
The
enzyme equally used
linoleic or
arachidonic acid as a substrate . -
pubmed
NDGA inhibited the
enzyme in vitro ( IC50 = 142 microM ) , but its addition in the exponential phase of growth activated the
enzyme . -
pubmed
The substrates for measuring the activity of individual
CYP in vitro included
phenacetin ,
tolbutamide ,
debrisoquine , and
omeprazole . -
pubmed
Both plant species were assessed for sticky chromosomes by Feulgen - stained mitotic analysis and flow cytometric analysis of
propidium iodide ( PI)-stained
G1 nuclei . -
pubmed
The neurotransmitter
L - glutamate also led to activation of
NF - kappaB and electrophysiology recordings showed a small but sustained increase in synaptic transmission ( fEPSP 106+/-12 % , 30 min
post - drug ) . -
pubmed
After a median of 48 ( range 2 - 126 ) weeks of therapy (
IFN , n=190 ;
IFN and
ribavirin , n=96 ;
pegylated IFN , n=20 ;
pegylated IFN and
ribavirin , n=26 ) , 93 patients ( 28 % ) achieved an SVR . -
pubmed
These studies demonstrate that
inhibitors of
PP1 can be used in a tissue- and developmental - specific manner to examine the developmental roles of
PP1 . -
pubmed
Anti -
thrombin , anti - platelet and anti -
vitamin K agents are common treatments given for the prevention of
thrombotic or embolic events . -
pubmed
Thiopental inhibits
tumor necrosis factor alpha - induced activation of
nuclear factor kappaB through suppression of
kappaB kinase activity . -
pubmed
The authors have recently reported that
thiopental inhibits activation of
nuclear factor ( NF ) kappaB , a
transcription factor implicated in the expression of many inflammatory genes . -
pubmed
METHODS : The authors tested
gamma - aminobutyric acid (
GABA ) , the
GABA(A ) antagonist
bicuculline , and the
GABA(B ) antagonist
dichlorophenyl - methyl - amino - propyl - diethoxymethyl - phosphinic acid (
CGP 52432 ) in combination with
thiopental for their influence on the activation of
NF - kappaB. -
pubmed
In addition , they investigated the direct effect of
thiopental on activated
NF - kappaB DNA binding activity . -
pubmed
In addition , the authors tested the effect of the structural
barbiturate analog pairs
thiopental -
pentobarbital and
thiamylal - secobarbital and of
thiopental in combination with the
thio - group containing chemical
dithiothreitol on the activation of
NF - kappaB. -
pubmed
RESULTS :
GABA did not inhibit
NF - kappaB activation , and the
GABA(A ) and
GABA(B ) antagonists
bicuculline and
CGP did not diminish the
thiopental - mediated inhibitory effect on
NF - kappaB activation . -
pubmed
Thiopental did not inhibit activated
NF - kappaB directly in a cell - free system . -
pubmed
The phosphorylation of
IkappaBalpha was prevented after incubation with 1,000 microg / ml
thiopental . -
pubmed
Thiobarbiturates ( 4 x 10(-3 ) m ) inhibited
NF - kappaB activity , whereas equimolar concentrations of the structural
oxyanalogs did not . -
pubmed
CONCLUSION :
Thiopental - mediated inhibition of
NF - kappaB activation is due to the suppression of
IkappaB kinase activity and depends at least in part on the thio - group of the
barbiturate molecule . -
pubmed
Using standard immunocytochemical and light - microscopic techniques , the distribution of
GABA - ergic neurons ( and their co - localized
calcium - binding proteins :
calbindin (
CB ) ,
calretinin (
CR ) and
parvalbumin (
PV ) ) was determined for each area . -
pubmed
These data confirmed that there were striking differences in the distribution of
GABA- ,
CB- ,
CR- and
PV - positive neurons . -
pubmed
Because the chemical structure of the interface plays an important role in the coating adhesion , we studied the interface between CaP and the polymers
polyethylene (
PE ) and
polydimethylsiloxane (
PDMS /
silicone rubber ) . -
pubmed
On
PE , a low
Ca / P ratio nearby the interface and a high amount of C - O bonds were found on both untreated and plasma pretreated PE . -
pubmed
Amylase ,
IgA ,
proline - rich proteins and the high - molecular - weight glycoproteins ,
agglutinins , were detected in all saliva tested . -
pubmed
Carbohydrate - reactive antibodies recognized the low - molecular weight
mucin ,
MUC 7 in submandibular / sublingual saliva only . -
pubmed
HORTON ' S SYNDROME , A
GENETIC DISEASE : The preferential association of the disease with some alleles pf the
HLA DR4 group has helped to emphasize the fundamental role of a few
amino acids of the second hypervariable area of the
HLA - DR molecule . -
pubmed
YB-1(+/- ) cells showed increased sensitivity to
cisplatin and
mitomycin C , but not to
etoposide , X - ray or UV irradiation , as compared to
YB-1(+/+ ) cells . -
pubmed
These bacteriocins are characterized by the presence , in their primary structure , of
post - translationally modified
amino acid residues ( i.e. -
pubmed
Class
II bacteriocins , containing three subclasses , consist of small peptides that do not bear any modified
amino acid residue . -
pubmed
Moreover , some of these
bacteriocins , namely ,
sakacin G ,
pediocin PA-1 ,
enterocin A ,
coagulin , and divercin V41 , are characterized by the presence of a second
disulfide bond in the C - terminal region . -
pubmed
RESULTS : As compared with
vitamin E , three doses of
PHAS ( 1.6 , 0.8 and 0.4 ml / d ) prolonged the survival time to
hypoxia and the
pole climbing time and increased the heat survival rate , and the 0.8 ml / d PHAS had the best effect . -
pubmed
In the group of 0.8 ml / d PHAS , the activity of
SOD and
CAT decreased less , and the contents of
lipofuscin and
MDA decreased significantly . -
pubmed
The key step involves the one -
pot reaction for
2 - arylbenzofurans ( 2 , 7 ) from
4 - hydroxyphenylacetone with
4 '- acetoxy-2 - chloro-2 -( methylthio ) acetophenone ( 1 ) and
2 - chloro-2 - methylthio -( 2 ' , 4 ' , 6 '- trimethoxy ) acetophenone ( 6 ) under Friedel - Crafts reactionconditions . -
pubmed
In vitro , inhibition of
NTE activity by
organophosphates is correlated with inhibition of neurite initiation and reduction of neurite length , supporting the hypothesis that
organophosphate - induced
neurological disorders are caused by inhibition of
NTE activity . -
pubmed
However , there is no direct evidence for the involvement of
NTE in
organophosphate - induced
impairment of neurites in vitro . -
pubmed
Liver X receptors (
LXRs ) are nuclear receptors that regulate macrophage
cholesterol efflux by inducing
ATP - binding cassette transporter A1 (
ABCA1 ) and
ABCG1 /
ABCG4 gene expression . -
pubmed
The
Niemann - Pick C ( NPC ) proteins
NPC1 and
NPC2 are located in the late endosome , where they control
cholesterol trafficking to the plasma membrane . -
pubmed
Synthetic
LXR activators increase the amount of free
cholesterol in the plasma membrane by inducing
NPC1 and
NPC2 gene expression . -
pubmed
Moreover ,
ABCA1 - dependent
cholesterol efflux induced by
LXR activators was drastically decreased in the presence of
progesterone , which blocks postlysosomal
cholesterol trafficking , and reduced when
NPC1 and
NPC2 mRNA expression was depleted using small interfering RNA . -
pubmed
The stimulation of
cholesterol mobilization to the plasma membrane by
LXRs led to a decrease in
cholesteryl ester formation and
Acyl - coenzyme A cholesterol acyltransferase-1 activity . -
pubmed
These data indicate that
LXR activation enhances
cholesterol trafficking to the plasma membrane , where it becomes available for efflux , at the expense of esterification , thus contributing to the overall effects of
LXR agonists in the control of macrophage
cholesterol homeostasis . -
pubmed
The minimal model ( MM ) of
glucose kinetics during an intravenous
glucose tolerance test ( IVGTT ) is widely used in clinical studies to measure metabolic indexes such as
glucose effectiveness ( S(G ) ) and
insulin sensitivity ( S(I ) ) . -
pubmed
Although the risk was observed originally with trials conducted with
rofecoxib , it was attributed generally to the entire class of
COX-2 - selective drugs based on a similar mechanism of action and a hypothesis that predicted the possibility of a prothrombotic effect of the drugs compared with nonselective NSAIDs . -
pubmed
In contrast , the genes for the controlling enzymes of
heme synthesis and degradation (
5 - aminolevulinate synthase 1 and
heme oxygenase 1 , respectively ) were up - regulated , implying depletion of a regulatory
heme pool . -
pubmed
Inhibition of
heme synthesis ( by 70 - 80 % ) at different enzymic steps by
succinyl acetone and
N - methylprotoporphyrin IX resulted in the earlier lowered expression of
NMDAzeta1 and -epsilon2 and
NF - L. -
pubmed
A single cotyledon was localized , perfused and stabilized with physiologic Eagles minimal essential medium containing 3 %
bovine albumin and
heparin as described by Chalier ( Chalier JC . -
pubmed
BNP as a marker of the
heart failure in the treatment of
imatinib mesylate . -
pubmed
Since its introduction 6 years ago ,
imatinib mesylate , a selective
tyrosine kinase inhibitor , has been a phenomenon in treating
chronic myelogenous leukemia (
CML ) with remarkably superior cytogenetic and molecular response rates at all stages of
CML followed by longer progression free survival . -
pubmed
The selected clones express
progesterone (
PR ) and
estrogen receptor - alpha (
ER - alpha ) at both mRNA and protein levels . -
pubmed
Two
16S rRNA gene libraries were constructed using the reverse transcription
polymerase chain reaction ( RT - PCR ) method to determine the major
ammonia oxidizers maintaining high cellular rRNA content . -
pubmed
In addition to this competition for
glyoxylate , competitive inhibition at the active site of
enzymes occurred between
glutamate and
alanine , but not between
serine and
glutamate or
alanine . -
pubmed
Phosphodiesterases (
PDEs ) regulate
cyclic nucleotide levels . -
pubmed
Increased
cyclic AMP (
cAMP ) signaling has been associated with
PRKAR1A or
GNAS mutations and leads to
adrenocortical tumors and
Cushing syndrome . -
pubmed
Tumor tissues showed 2q31 - 2q35 LOH , decreased protein expression and high
cyclic nucleotide levels and
cAMP - responsive element binding protein (
CREB ) phosphorylation . -
pubmed
PDE11A codes for a dual - specificity
PDE that is expressed in adrenal cortex and is partially inhibited by
tadalafil and other
PDE inhibitors ; its germline inactivation is associated with
adrenocortical hyperplasia , suggesting another means by which dysregulation of
cAMP signaling causes
endocrine tumors . -
pubmed
The least - correlated parameters were SRAX3 ,
TOP magnitude , and II3 in the
KCN group and I - S
K3 , amount of
astigmatism and MPE magnitude in the normal group . -
pubmed
Effects of
ERBB2 amplicon size and genomic alterations of chromosomes 1 , 3 , and 10 on patient response to
trastuzumab in metastatic
breast cancer . -
pubmed
In 35 patients with
ERBB2 - amplified metastatic
breast cancer , with 40 % overall response to
trastuzumab , fluorescence in situ hybridization identified the telomeric extent of the
ERBB2 amplicon and the status of the several pathway - related genes . -
pubmed
These results suggest that patient selection for
trastuzumab may be improved by considering
ERBB2 amplicon size and genomic status of the 1q25 ,
PTGS2 , and centromere 10 loci . -
pubmed
Then , the solid complexes of
genistein with these macrocycles in 1:1 molar ratio were prepared by the co - precipitation method and characterized by FTIR absorption spectroscopy in
ATR geometry . -
pubmed
The protein , designated
Cancerous Inhibitor of PP2A (
CIP2A ) , interacts directly with the oncogenic transcription factor
c - Myc , inhibits
PP2A activity toward
c - Myc serine 62 ( S62 ) , and thereby prevents
c - Myc proteolytic degradation . -
pubmed
Taken together ,
PF4 - stimulated immediate monocyte functions (
oxygen radical formation ) are regulated by
p38 MAPK ,
Syk , and
PI3 K , whereas delayed functions ( survival and
cytokine expression ) are controlled by
Erk and
JNK . -
pubmed
The reactions of a model
sulfur ylide with
formaldehyde and
1,1 - dicianoethylene , leading to the formation of an
epoxyde and a
cyclopropane , respectively , have been studied using different computational methods , and the results have been compared to those obtained with the
CBS - QB3 method . -
pubmed
Monomer formation and function of
p - hydroxybenzoate hydroxylase in reverse micelles and in
dimethylsulfoxide / water mixtures . -
pubmed
Surprisingly , highly conserved consensus sites for binding
post - synaptic density-95 / discs large / zonula occludens-1 - domain - containing ( PDZ ) proteins and
serines with a high probability of phosphorylation play no significant role in myoblast fusion . -
pubmed
Biochemical studies establish that the
SNS cytodomain is phosphorylated at multiple
tyrosines and their site - directed mutagenesis compromises the ability of the corresponding transgenes to rescue myoblast fusion . -
pubmed
This complexity and redundancy of multiple critical sequences within the
SNS cytodomain suggest that it functions through a complex array of interactions that likely includes both
phosphotyrosine - binding and SH3 - domain - containing proteins . -
pubmed
The clinical symptoms , serum levels of
triglyceride (
TG ) and APACHE
II scores were observed before and after DFPP . -
pubmed
In the presence of
pronase E , for example ,
glycoproteins are digested to small
glycopeptides having one to six
amino acids residues , which can be analyzed with excellent sensitivity using mass spectrometry . -
pubmed
When digesting
glycoproteins ,
pronase E produced glycopeptides within 5min under microwave irradiation ; glycopeptides having one or two
amino acids were the major products . -
pubmed
Although analysis of peptides containing multiple
amino acid residues offers the opportunity for peptide sequencing and provides information regarding the sites of glycosylation , the signals of
Asn - linked glycans were often suppressed by the
glycopeptides containing basic
amino acids (
Lys or
Arg ) in MALDI -
MS experiments . -
pubmed
This feature can be used to elucidate
glycan structures and glycosylation sites without the need for
MS /
MS analysis . -
pubmed
After ipsilateral injections of
biotinylated dextran amine (
BDA ) into the CeA and
cholera toxin B subunit (
CTb ) into the NRA , the prominent overlapping distribution of
BDA - labeled axon terminals and
CTb - labeled neurons was found ipsilaterally in the lateral / ventrolateral
PAG , where some of the
BDA - labeled terminals made symmetrical synaptic contacts with
somata and dendrites of the
CTb - labeled neurons . -
pubmed
After
BDA injection into the lateral / ventrolateral
PAG ,
BDA - labeled fibers were distributed mainly in and around the NRA within the medulla oblongata . -
pubmed
CA was induced in vitro in a medium containing BSA ,
CaCl ( 2 ) ,
NaHCO ( 3 ) , and
AR by the addition of the
calcium ionophore
A23187 . -
pubmed
Flow cytometry ( FC ) analyses were performed by loading samples with
Fluo-3 AM , that emits fluorescence at a high [
Ca ( 2 +)]( i ) , combined with
propidium iodide (
PI ) that allowed us to discriminate sperm with / without an integral plasma membrane both with high / low [
Ca ( 2 +)]( i ) . -
pubmed
The treatment of sperm with increasing concentrations of two
CaM antagonists , W7 and
calmidazolium (
CZ ) , accounted for an increase in capacitated and acrosome - reacted CTC - sperm patterns . -
pubmed
After
AR , only 7.2 % of the total sperm preserved intact membranes with a very high [
Ca(2+)](i ) . -
pubmed
Elucidation of phototransformation reactions of the X - ray contrast medium
iopromide under simulated solar radiation using UPLC - ESI - QqTOF -
MS . -
pubmed
Following a 120 - min irradiation period , an almost complete primary degradation of
iopromide gave rise to a series of photoproducts that were chromatographed on a reversed - phase UPLC and subsequently characterized by a combination of accurate mass measurements on a ESI - QqToF -
MS instrument and H / D - exchange experiments . -
pubmed
Translational fusions of
lacZ to each
rsbR paralogue revealed that
rsbRA , -
RB and -
RC are expressed at similar levels , which remain relatively constant during growth ,
ethanol stress and entry into stationary phase . -
pubmed
rsbRD , in contrast , is expressed at a level that is only slightly above background during growth , but is induced to 30 % of the
rsbRA expression level following
ethanol stress . -
pubmed
The evolutionary conserved sorting and assembly machinery ( SAM complex ) assembles mitochondrial beta - barrel proteins , such as
voltage - dependent anion - selective channel 1 (
VDAC1 ) , into complexes in the outer membrane by recognizing a sorting beta - signal in the
carboxy - terminal part of the protein . -
pubmed
The length of the tag at the C - terminus is critical for the assembly of
VDAC1 , as well as the
amino acid residues at positions 130 , 222 , 225 and 251 of the protein . -
pubmed
STAT3 inhibitor
WP1066 as a novel therapeutic agent for
renal cell carcinoma . -
pubmed
We examined whether it could be a novel therapeutic target for
RCC by using the
STAT3 inhibitor
WP1066 . -
pubmed
WP1066 suppressed the expression of
Bcl-2 , induced apoptosis , and inhibited the basal and
hypoxia - induced expression of
HIF1alpha and
HIF2alpha , as well as
vascular endothelial growth factor secretion into cell culture medium . -
pubmed
CONCLUSION : Our results suggest that using
WP1066 to inhibit the
STAT3 signalling pathway could be a novel therapeutic strategy against
RCC . -
pubmed
Paclitaxel was administered at 120 mg / m(2 ) on day 1 and S-1 was administered orally at 80 mg / m(2 ) for 14 consecutive days , followed by a 1 - week
rest , as one course . -
pubmed
Expressions of
biopyrrins and
heme oxygenase-1 (
HO-1 ) , a stress - responsive
bilirubin - producing enzyme , in heart , aorta , kidney , liver and lung were immunostained with autopsied specimens . -
pubmed
Immunoreactive
HO-1 and
biopyrrins were also observed in renal tubular cells , aortic wall and lung . -
pubmed
Induction of anti - oxidative enzyme
HO-1 seemed to be involved in the activation of
bilirubin /
biopyrrin pathway . -
pubmed
In contrast , the kinetics of
c - myc and
c - Ha - ras inducibility were enhanced by
BaP ( 3 mum ) leading to significant enhancement of mRNA levels . -
pubmed
Challenge with
2,3,7,8 - tetrachlorodibenzo - p - dioxin (
TCDD ) ( 1 nm ) , an
aryl hydrocarbon receptor (
AhR ) agonist , elicited responses comparable to
BaP for
c - jun and
c - Ha - ras , but not for c - fos or
c - myc . -
pubmed
alpha - naphthoflavone ( 10 nm ) , an antagonist / agonist of the
AhR and inhibitor of
cytochrome P450 , elicited
c - jun and
c - Ha - ras responses comparable to
BaP. -
pubmed
Pretreatment of the cells with this agent nullified the modulatory effects of
BaP on c - fos and
c - myc . -
pubmed
Carbohydrate decoration of microporous
polypropylene membranes for
lectin affinity adsorption : comparison of
mono - and disaccharides . -
pubmed
As the BD of
saccharide increases , the "
glycoside cluster effect " plays a primary role in
lectin adsorption . -
pubmed
Bz-423 , a small - molecule inhibitor of the
mitochondrial F ( 1 ) F ( 0 ) adenosine triphosphate synthase ( F ( 1 ) F ( 0 )- ATPase ) , selectively increased
superoxide and induced the apoptosis of alloreactive T cells , which arrested established
GVHD in several
BMT models without affecting hematopoietic engraftment or lymphocyte reconstitution . -
pubmed
Alpha - synuclein (
SNCA ) is associated with a range of
psychiatric diseases including
neurodegeneration ,
alcohol craving , and
depression . -
pubmed
BACKGROUND AND PURPOSE : The
cystic fibrosis transmembrane conductance regulator (
CFTR ) is a
cAMP - dependent
chloride channel in the plasma membrane of epithelia whose mutation is the cause of the
genetic disease cystic fibrosis (
CF ) . -
pubmed
The most frequent
CFTR mutation is deletion of
Phe(508 ) and this mutant protein (
delF508CFTR ) does not readily translocate to the plasma membrane and is rapidly degraded within the cell . -
pubmed
CONCLUSION AND IMPLICATIONS : These results show that
resveratrol modulated
CFTR expression and localization and could rescue
cAMP - dependent
chloride transport in delF508CFTR cells . -
pubmed
PRINCIPAL FINDINGS : The paper summarizes the general findings including various classes of compounds , and more specific information on two veterinary anthelmintics (
monepantel ,
emodepside ) and
nitazoxanide , an antiprotozoal drug , compiled from the
EMA EPAR and FDA registration files . -
pubmed
The levels of
serum pituitary gonadotropin including
estradiol ,
follicle - stimulating hormone ,
luteinizing hormone ,
prolactin and
testosterone were measured before treatment and 3 months , 6 months and 1 year after treatment . -
pubmed
Interface pressures ( IPs ) and transcutaneous blood gas levels [ transcutaneous tissue
oxygen ( T(c ) PO(2 ) ) ] were simultaneously assessed over both ischia and the sacrum to investigate the hypotheses : ( i ) tissue oxygenation decreases with sustained applied pressure ; ( ii ) tissue
oxygen and
IP are inversely correlated in loaded soft tissues ; ( iii ) multisite assessments are unnecessary because healthy individuals are symmetrical . -
pubmed
The medium chain
triglyceride (
MCT ) ketogenic diet is used extensively for treating refractory childhood
epilepsy . -
pubmed
We identify specific medium chain
fatty acids ( both prescribed in the
MCT diet , and related compounds branched on the fourth
carbon ) that provide significantly enhanced in vitro
seizure control compared to
VPA . -
pubmed
The activity of these compounds on
seizure control is independent of
histone deacetylase inhibitory activity ( associated with the teratogenicity of
VPA ) , and does not correlate with
liver cell toxicity . -
pubmed
Our data therefore implicates medium chain
fatty acids in the mechanism of the
MCT ketogenic diet , and highlights a related new family of compounds that are more potent than
VPA in
seizure control with a reduced potential for side effects . -
pubmed
Adding 3 %
Tween 20 or 1 %
catalase enabled cells to become culturable again , with resuscitation times of 48 h and 24 h , respectively . -
pubmed
Because
UGT2B17 metabolizes certain nonsteroidal anti - inflammatory drugs and
flavonoids with antioxidative properties , individuals with a gene deletion may have higher levels of these protective dietary components . -
pubmed
Glucose is an energy source that also controls the expression of key genes involved in energetic metabolism through the
glucose - signaling transcription factor
carbohydrate response element - binding protein (
ChREBP ) . -
pubmed
ChREBP has recently emerged as a central regulator of glycolysis and de novo
fatty acid synthesis in liver , but new evidence shows that it plays a broader and crucial role in various processes , ranging from glucolipotoxicity to apoptosis and/or proliferation in specific cell types . -
pubmed
However , several aspects of
ChREBP activation by
glucose metabolites are currently controversial , as well as the effects of activating or inhibiting
ChREBP , on
insulin sensitivity , which might depend on genetic , dietary or environmental factors . -
pubmed
Crescentic
glomerulonephritis due to
IgA nephropathy or
propylthiouracil - induced
Mpo - Anca positive
vasculitis ? -
pubmed
Currently , the best - suited piezoelectric material for high - temperature SAW applications is
langasite (
LGS ) . -
pubmed
A gas chromatography (
GC ) method for the analysis of
pymetrozine was developed after utilizing matrix enhancement effect of
pymetrozine to
nitrogen phosphorus detector (
NPD ) . -
pubmed
Samples were extracted with
acetonitrile and purified through
primary secondary amine (
PSA ) and
C18 dispersive sorbent . -
pubmed
Estrogen insufficiency decreases defense against oxidative stress ; skin becomes thinner with less
collagen , decreased elasticity , increased wrinkling , increased dryness and reduced vascularity . -
pubmed
The relevance of
estrogen replacement , selective
estrogen receptor modulators ( SERMs ) and phytoestrogens as therapies for diminishing skin aging is highlighted . -
pubmed
The datasets were derived using Illumina high - throughput sequencing and were based on BLAST against self - established databases of BDGs ,
DDT degradation genes (
DDGs ) ,
HCH degradation genes (
HDGs ) , and
ATZ degradation genes ( ADGs ) . -
pubmed
The
hdt ,
hdg , and
atzB genes , which encode for
hydratase ,
dehalogenase , and
ethylaminohydrolase , were found to be the most abundant genes involved in
DDT ,
HCH , and
ATZ degradation , respectively . -
pubmed
Here , we examine
oxygen - deficient H :
TiO2 nanotube arrays that have previously achieved very high solar - to -
hydrogen (
STH ) efficiencies due to incident photon - to - current efficiency ( IPCE ) values of & gt;90 % for photoelectrochemical water splitting at only 0.4 V vs
RHE under UV illumination . -
pubmed
Neuroglobin is a brain globin with neuroprotective effects against
ischemia and related
pathological processes , acting as
O2 sensor and antiapoptotic pathway transducer . -
pubmed
The one -
pot reaction involving a
Cu ( I )/ Pd ( 0 ) cooperative dual catalyst directly addresses the need to develop more time - efficient and less - wasteful synthetic pathways . -
pubmed
Defining a therapeutic window for the novel
TGF - beta inhibitor
LY2157299 monohydrate based on a pharmacokinetic / pharmacodynamic model . -
pubmed
AIMS : To identify prospectively a safe therapeutic window for administration of a novel oral
transforming growth factor beta (
TGF - beta ) inhibitor ,
LY2157299 monohydrate , based on a pharmacokinetic / pharmacodynamic ( PK / PD ) model . -
pubmed
Insulin is widely used to control posttrauma
glucose , but this treatment increases the risk of
hypoglycemia . -
pubmed
We tested a novel method for early posttrauma
hyperglycemia control by suppressing hepatic glycogenolysis via
beta2 - adrenoreceptor blockade [
ICI-118551 ( ICI ) ] . -
pubmed
AIM OF THE STUDY : End tidal
carbon dioxide ( EtCO2 ) monitoring is considered to reflect real - time estimation of partial pressure of
carbon dioxide in arterial blood ( PaCO2 ) noninvasively . -
pubmed
Consequently , the antagonistic
A2A - D2 receptor interactions in a putative striatal receptor heteromer on striato - pallidal
GABA neuron led to the introduction of
A2A receptor antagonists as possible anti-
Parkinsonian drugs . -
pubmed
Special emphasis is given to in vivo microdialysis findings demonstrating the functional role putative
A2A /
D2 heteromers on striato - pallidal
GABA neurons play in the modulation of this pathway , in which
A2A receptors inhibit
D2 receptor signaling . -
pubmed
However , this does not necessarily lead to
type I IFN production or autoimmunity unless there is concomitant exposure to endogenous
TLR-7 ligands , which are released from dead cells following
pristane treatment . -
pubmed
Circadian changes in
phosphate were not explained by urine
phosphate excretion ,
parathyroid hormone , or
fibroblast growth factor-23 . -
pubmed
The main such transporters include
Na (+)/ H (+ ) exchangers , various
HCO3 (- ) transporters ,
H (+ ) pumps , and
lactate - H (+ ) cotransporters . -
pubmed
A water - soluble cationic conjugated polyelectrolyte (
CPE ) ,
poly ( 1,4 - bis ( 6 -( 1 - methylimidazolium )- hexyloxy )- benzene bromide ) (
PMI ) displays extraordinary stability over the full pH range of 1 - 14 as well as in seawater , brine , urine , and other solutions and carries out efficient detection , discrimination , and removal of moderately dissimilar anionic surfactants ( viz . -
pubmed
Using
PMI , a newly developed
CPE , a rapid and practical method for the efficient detection , discrimination , and removal of
SDS and SDBS at ppb levels from water and urine , under harsh conditions , and in natural chemical environments is demonstrated . -
pubmed
In the
MTA group , none of the mixing techniques exhibited a significant effect on
CS ( P>0.05 ) ; however , in CEM group the
CS at 21 - h and 21 - day intervals was higher with the hand technique ( P<0.05 ) . -
pubmed
Telescopic vs. balloon showed :
post - operative
pain - day one 2.5 vs. 2.5 ; p = 0.90 , day seven 0 vs. 0 ; p = 0.02 ( 0 vs. 1 ; p & lt ; 0.01 for bilateral
hernias ) ; operation times - unilateral 48.0 vs. 48.0 min ; p = 0.88 and bilateral 70.0 vs. 65.0 min ; p = 0.66 , length of hospital stay 1.0 vs. 1.0 day , analgesic intake (
dextropropoxyphene ) 6.0 vs. 6.0 tablets ; p = 0.95 , return to work / normal physical activities 7.0 vs. 7.0 days ; p = 0.46 and cosmetic
scar scores 24.0 vs. 24.0 , respectively . -
pubmed
This response could be mimicked by the addition of
Asymmetric Dimethylarginine (
ADMA ) , an endogenous inhibitor of
NO synthesis and the substrate for both isoforms of
DDAH . -
pubmed
WIDER IMPLICATIONS OF THE FINDINGS : This study provides new insight into the role of the
DDAH /
ADMA pathway in the regulation of trophoblast function . -
pubmed
Both dysregulation of
DDAH and the accumulation of
ADMA have been associated with the development of
pre - eclampsia . -
pubmed
This is the first study to implicate the
DDAH /
ADMA pathway as a mechanism that might underlie the poor trophoblast invasion seen in this common
pregnancy disorder . -
pubmed
STIM1 elevation in the heart results in aberrant
Ca2 + handling and
cardiomyopathy . -
pubmed
Stromal interaction molecule 1 (
STIM1 ) is a
Ca ( 2 + ) sensor that partners with
Orai1 to elicit
Ca ( 2 + ) entry in response to endoplasmic reticulum (
ER )
Ca ( 2 + ) store depletion . -
pubmed
While store - operated
Ca ( 2 + ) entry ( SOCE ) is important for maintaining
ER Ca ( 2 + ) homeostasis in non - excitable cells , it is unclear what role it plays in the heart , although
STIM1 is expressed in the heart and upregulated during disease . -
pubmed
As expected ,
STIM1 transgenic myocytes showed enhanced
Ca ( 2 + ) entry following store depletion and partial co - localization with the
type 2 ryanodine receptor (
RyR2 ) within the sarcoplasmic reticulum (
SR ) , as well as enrichment around the sarcolemma . -
pubmed
Associated with this enhanced
Ca ( 2 + ) profile was an increase in cardiac nuclear factor of activated T - cells (
NFAT ) and
Ca ( 2 +)/ calmodulin - dependent kinase II (
CaMKII ) activity . -
pubmed
We conclude that
STIM1 has an unexpected function in the heart where it alters communication between the sarcolemma and
SR resulting in greater
Ca ( 2 + ) flux and a leaky SR compartment . -
pubmed
Second - Trimester Maternal Serum
Paraxanthine ,
CYP1A2 Activity , and the Risk of Severe
Preeclampsia . -
pubmed
OBJECTIVE : To measure the association between second - trimester maternal
caffeine intake and
caffeine metabolism through the
CYP1A2 system and the risk of subsequent severe
preeclampsia . -
pubmed
The outcome of interest was severe
preeclampsia , and exposures were serum
paraxanthine (
1,7 - dimethylxanthine ) , measured through high - performance liquid chromatography , and
CYP1A2 activity , assessed by
paraxanthine /
caffeine ratios . -
pubmed
In erythrocytes made echinocytic with exogenous phospholipid , addition of
hemoglobin oxidized with
hydrogen peroxide (
H2O2 ) or
Vitamin C ( conditions that result in the formation of significant quantities of choleglobin ) , but not
ferricyanide ( which produces predominantly methemoglobin ) , induced dose - dependent shape reversion to less echinocytic forms , consistent with extraction of phospholipids from the exofacial side of the membrane . -
pubmed
Erythrocytes preloaded with
radiolabeled phosphatidylcholine or
NBD - labeled phosphatidylcholine ,
phosphatidylglycerol or
phosphatidic acid , exhibited greatest extraction of radiolabel or fluorescence signal with exogenous
hemoglobin oxidized via
H2O2 or
Vitamin C , but not
ferricyanide . -
pubmed
However , with
NBD - phosphatidylserine ( a preferential inner monolayer intercalator ) , significantly less extraction of labeled lipid occurred with oxidized
hemoglobin prepared under all three oxidizing conditions . -
pubmed
In
dimyristoylphosphatidylcholine liposomes containing
radiolabeled phosphatidylcholine ,
phosphatidylserine or
phosphatidylethanolamine , subsequent addition of
hemoglobin oxidized with
H2O2 or
Vitamin C extracted radiolabeled lipid with significantly greater efficiency than
ferricyanide - treated
hemoglobin , with enhanced extraction detectable at levels approaching physiological plasma oxidant concentrations . -
pubmed
Hemoglobin dimerization occurred at very low levels with
H2O2 treatment , and even lower levels with
Vitamin C treatment , and thus did not correlate to the high efficiency and consistent levels of lipid extraction observed with these treatments . -
pubmed
These findings indicate that
choleglobin extracts lipids from cell membranes regardless of headgroup or
acyl chain length , through a process of direct hydrophobic interaction with the membrane surface . -
pubmed
The immunological effects of
rapamycin and BM -
MSC treatments were associated with the inhibition of the
Ag - specific lymphocyte proliferation ,
CD8 + cytolytic activity , and the Th1 - type
cytokine (
gamma - interferon (
IFN - gamma ) ) and the increase of
Th-2 cytokine (
interleukin-4 (
IL-4 ) and
IL-10 ) production . -
pubmed
This suggests the potential of
rapamycin and BM -
MSC combined therapy to play neuroprotective roles in the treatment of
neuroinflammatory disorders . -
pubmed
The blood
glucose (
BG ) level over a 30 - minute interval in Week 2 was relatively the same as that in Week 1 , suggesting that EA treatment does not increase the likelihood of developing
hyperglycemia . -
pubmed
BACKGROUND : The aim of this study was to investigate the relationship between serum
triglycerides (
TG ) levels and
atherosclerosis and to explore its predicated value for
atherosclerosis in elderly Chinese population . -
pubmed
The aim of this study was to investigate whether
CS /
DS rich in
GalNAc(
4,6SO4 ) residues participate in the progression or resolution of
liver fibrosis . -
pubmed
ATM /
CHK /
p53 Pathway Dependent Chemopreventive and Therapeutic Activity on
Lung Cancer by
Pterostilbene . -
pubmed
To investigate the underlying mechanism of this superior action of
pterostilbene on
cancer cells , we utilized a reverse - phase protein array followed by bioinformatic analysis and found that the
ATM /
CHK pathway is modified by
pterostilbene in a
lung cancer cell line . -
pubmed
Given that
ATM /
CHK signaling requires
p53 for its biological effects , we hypothesized that
p53 is required for the anticancer effect of
pterostilbene . -
pubmed
Pterostilbene also activated
ATM and
CHK1 / 2 , which are upstream of
p53 , in both cell lines , though
pterostilbene - induced senescence was dependent on the presence of
p53 . -
pubmed
Finally ,
pterostilbene more effectively inhibited
p53 - dependent cell proliferation compared to the other three
stilbenoids . -
pubmed
These results strongly support the potential chemopreventive effect of
pterostilbene on
p53 - positive cells during early
carcinogenesis . -
pubmed
Hydrostatic pressure applied using
diamond anvil cells (
DAC ) has been widely explored to modulate physical properties of materials by tuning their lattice degree of freedom . -
pubmed
Multiple - terminal FETs were fabricated in the
DAC using few - layer
MoS2 and
BN as the channel semiconductor and dielectric layer , respectively . -
pubmed
We intended to identify a set of structurally diverse hits , which occupy all subsites ( S1'-S3 ' , S2 , and
S3 ) centering the
zinc containing binding site of
MMP-13 , by the virtual screening of a chemical library comprising more than ten million commercially available compounds . -
pubmed
Exogenous
diacylglycerol leads to membrane translocation of
PKC , indicative of an activation . -
pubmed
The intima and media layers were obtained by sequentially electrospinning silk fibroin (
SF ) and
poly ( L - lactide - co - epsilon - caprolactone ) , with ratios selected to match the mechanical properties of the native tissue . -
pubmed
The discovery that the most abundant
cholesterol oxidized metabolite in the plasma ,
27 hydroxycholesterol (
27 - OHC ) , is a selective
estrogen receptor modulator ( SERM ) and an agonist of
Liver X receptors (
LXR ) partially fills the void in our understanding and knowledge of the mechanisms that may link
hypercholesterolemia to development and progression of
breast cancer and
PCa . -
pubmed
The wide spectrum and repertoire of SERM and
LXR - dependent effects of
27 - OHC in the context of all facets and aspects of
breast cancer and
prostate cancer biology are reviewed in this manuscript in a very comprehensive manner . -
pubmed
This review highlights recent findings pertaining to the role of
27 - OHC in
breast cancer and
PCa and delineates the signaling mechanisms involved in the governing of different facets of
tumor biology , that include
tumor cell proliferation , epithelial - mesenchymal transition (
EMT ) , as well as
tumor cell invasion , migration , and metastasis . -
pubmed
The plasma
H2S levels , apoptosis of esophageal - gastric junction vascular smooth muscle cells , and the expression of
nuclear transcription factor - kappaB (
NF - kappaB ) , p -
AKT ,
IkappaBa and
Bcl-2 were detected . -
pubmed
The results showed that the plasma
H2S levels and the
CSE expression levels had statistically significant difference among different groups ( P<0.05 ) . -
pubmed
As compared with PH group , plasma
H2S levels were declined obviously ( 11.9 + -4.2 vs. 20.6 + -4.5 , P<0.05 ) , and
CSE expression levels in the junction vascular tissue were notably reduced ( 1.7 + -0.6 vs. 2.8 + -0.8 , P<0.05 ) , apoptosis rate of vascular smooth muscle cells per unit area was significantly decreased ( 0.10 + -0.15 vs. 0.24 + -0.07 , P<0.05 ) , and the expression levels of p -
AKT and
NF - kappaB were significantly decreased ( 2.31 + -0.33 vs. 3.04 + -0.38 , P<0.05 ; 0.33 + -0.17 vs. 0.51 + -0.23 , P<0.05 ) , however ,
IkappaBa and
Bcl-2 expression increased obviously ( 5.57 + -0.17 vs. 3.67 + -0.13 , P<0.05 ; 0.79 + -0.29 vs. 0.44 + -0.36 , P<0.05 ) in PH+
PPG group . -
pubmed
As compared with PH group ,
H2S levels were notably increased ( 32.7 + -7.3 vs. 20.6 + -4.5 , P<0.05 ) , the
CSE levels in the junction vascular tissue were significantly increased (
6.3 + -0.7 vs. 2.8 + -0.8 , P<0.05 ) , apoptosis rate of vascular smooth muscle cells per unit area was significantly increased ( 0.35 + -0.14 vs. 0.24 + -0.07 , P<0.05 ) , and the expression levels of p -
AKT and
NF - kappaB were significantly increased ( 4.29 + -0.49 vs. 3.04 + -0.38 , P<0.05 ; 0.77 + -0.27 vs. 0.51 + -0.23 , P<0.05 ) , yet
IkappaBa and
Bcl-2 expression decreased significantly ( 3.23 + -0.24 vs. 3.67 + -0.13 , P<0.05 ; 0.31 + -0.23 vs. 0.48 + -0.34 , P<0.05 ) in PH+S group . -
pubmed
H2S can activate
NF - kappaB by the p -
AKT pathway , leading to the down - regulation of
Bcl-2 , eventually stimulating apoptosis of vascular smooth muscle cells , easing PH . -
pubmed
H2S / CSE system may play an important role in remission of
PH via the
AKT -
NF - kappaB pathway . -
pubmed
MR is expressed in all cell types in the heart , including the endothelial cells ( EC ) , in which
aldosterone induces the expression of
intercellular adhesion molecule 1 (
ICAM-1 ) . -
pubmed
This pregnancy - related analgesia is reversible by intrathecal administration of
naloxone , suggesting an opioid - mediated mechanism ; pharmacological and genetic data suggest the importance of
delta - opioid receptors . -
pubmed
We show that the phenomenon is dependent on pregnancy hormones (
estrogen and
progesterone ) ,
delta - opioid receptors , and T cells of the adaptive immune system . -
pubmed
The extracellular , active 180
amino acid Nogo - A region , named
Nogo - A - Delta20 , binds to heparin and brain - derived heparan sulfate glycosaminoglycans ( GAGs ) but not to the closely related chondroitin sulfate
GAGs . -
pubmed
Ultrasound - assisted facile one -
pot sequential synthesis of novel
sulfonamide - isoxazoles using
cerium ( IV ) ammonium nitrate (
CAN ) as an efficient oxidant in aqueous medium . -
pubmed
A series of novel
3,5 - disubstituted isoxazoles have been synthesized , using a new , green , and versatile " one -
pot three - steps " methodology . -
pubmed
The syntheses are highly regioselective , as illustrated by the structures of the final compounds , which have been fully assessed by spectral analyses (
1H and
13C NMR ,
MS ) . -
pubmed
GP : T and DN :P
SI ratios were compared between groups by using the analysis of variance and were analyzed relative to group , total cumulative number of doses of
GBCA , age , and sex by using multivariable linear models . -
pubmed
Concerted action of
dipeptidyl peptidase IV and
glutaminyl cyclase results in formation of
pyroglutamate - modified amyloid peptides in vitro . -
pubmed
The subsequent transformation of resulted
Abeta ( 3 - 40 / 42 ) to pE -
Abeta ( 3 - 40 / 42 ) in
QC catalyzed
glutamate cyclization was manifested . -
pubmed
The best mucilage production from callus ( 36.76 % g g-1 dry weight ) was obtained in the
MS medium supplemented with 1 mg L-1 of 2 , 4 - D and 2 mg L-1 of
BAP under the light condition . -
pubmed
The supplementation of emulsifier in the low - energy diet linearly decreased
cholesterol ( P = 0.099 ) and
LDL / C ( P = 0.074 ) . -
pubmed
Based on the
BAS score , BD who were moderately
BAS - affected ( n = 5 ) , had significantly lower arterial saturation of
hemoglobin with
oxygen values on arterial blood gas when compared with
MDD ( n = 18 ) . -
pubmed
Although
TRPV1 expressing neuron percentage was increased in
Ins2+/Akita DRGs at 9 months of
diabetes compared to control ,
capsaicin - induced
Ca2 + influx was smaller in isolated
Ins2+/Akita DRG neurons , indicating impaired
TRPV1 function . -
pubmed
Patch -
clamp recordings revealed that
capsaicin - activated currents decayed faster in isolated
Ins2+/Akita DRG neurons as compared to those in control neurons . -
pubmed
We propose that in poorly controlled
diabetes , the accelerated rate of
capsaicin - sensitive
TRPV1 current decay in DRG neurons decreases overall
TRPV1 activity and contributes to
peripheral neuropathy . -
pubmed
It has been shown previously in
formaldehyde crosslinking experiments that
EloR co - precipitates with
KhpA , indicating that they form a complex in vivo . -
pubmed
Site directed mutagenesis identified
isoleucine 61 ( I61 ) as crucial for
KhpA homodimerization . -
pubmed
When substituting I61 with
phenylalanine ,
KhpA lost the ability to homodimerize , while it still interacted clearly with
EloR. -
pubmed
Novel
PSMA - Coated On - Off - On Fluorescent Chemosensor Based on Organic Dots with AIEgens for Detection of
Copper ( II ) ,
Iron ( III ) and
Cysteine . -
pubmed
The active
esters in the non - conjugated
vinyl monomers could be used in a
post - polymerization functionalization step to create functionalized polymers with defined monomer sequences in a modular way . -
pubmed
The porosity of the cement paste , the aggregate and the interfacial transitional zones ( ITZ ) of a PC with recycled coarse aggregates ( RCA ) and
silica fume (
SF ) is measured by means of image analysis - scanning electron microscope ( IA)-(SEM ) and by mapping the chemical elements with an SEM - EDS ( energy dispersive spectrometer ) detector microanalysis linked to the SEM and , as a contrast , the
mercury intrusion porosimetry technique (
MIP ) . -
pubmed
Cadmium is an environmental contaminant that can activate
estrogen receptor alpha (
ERalpha ) and contribute to the development and progression of
breast cancer . -
pubmed
MTT and
TTP were significantly higher and
CBF was significantly lower when the number of LSAs was between 5 and 10 on each side of the basal ganglia ( P & lt ; 0.001 , & lt;0.001 , and & lt;0.001 , respectively ) . -
pubmed
The three most prevalent serovars in the
MAT were selected for the development of a pooled antigen for use in MSAT ; subsequently , the 193 serum were assessed with the macroscopic slide agglutination test ( MSAT ) containing the developed
antigen . -
pubmed
Celastrol - induced degradation of
FANCD2 sensitizes pediatric
high - grade gliomas to the DNA - crosslinking agent
carboplatin . -
pubmed
FINDINGS :
FANCD2 is overexpressed in
HGGs and depletion of
FANCD2 by
celastrol synergises with
carboplatin to induce
cytotoxicity . -
pubmed
In addition , our results suggest that
celastrol treatment stalls ongoing replication forks , causing sensitivity to DNA - crosslinking in
FANCD2 - dependent
glioma cells . -
pubmed
lncRNA
ZEB1 -
AS1 inhibits high
glucose - induced EMT and fibrogenesis by regulating the miR-216a-5p /
BMP7 axis in
diabetic nephropathy . -
pubmed
lncRNA
ZEB1 -
AS1 inhibited high
glucose - induced EMT and fibrogenesis via regulating miR-216a-5p /
BMP7 axis in
diabetic nephropathy , providing a potential target for DN therapy . -
pubmed
Zinc ferrite and
copper oxide nanostructure (
ZnFe2O4 -
CuO ) and gold nanoparticles ( AuNPs ) have been used to prepare a nanocomposite in modifying a
glassy carbon electrode (
GCE ) . -
pubmed
The unique
ZnFe2O4 -
CuO /
Au nanocomposite was applied as a sensor for the determination of traces of
MET by some electroanalytical techniques . -
pubmed
Under the optimum conditions and at a working potential of 0.85 V ( vs.
Ag /
AgCl/3.0 M
KCl ) , the sensor response is linear in the
MET range of 1.0 nmol L-1 to 1.0 mumol L-1
MET . -
pubmed
Hydrogenase genes were also widespread in samples from three other climatically distinct deserts , the Namib , Gobi , and Mojave , and atmospheric
H2 oxidation was also greatly stimulated by hydration at these sites . -
pubmed
AIM : This study aimed to assess the effect of addition of nano -
titanium oxide ( nano -
TiO2 ) , nano -
silicon dioxide ( nano -
SiO2 ) , and a combination of both , on antimicrobial activity of an orthodontic composite . -
pubmed
Warming crude ratuterine cytosol after the addition of
[ 3H ] estradiol accelerates the association of the
4 - S estrogen - binding protein with a second macromolecule , resulting in the formation of the
5 - S estrogen - binding protein . -
pubmed
Following each of these methods , the fraction containing the
4 - S estrogen - binding protein was incubated at 28 degrees C ; each of the these
4 - S estrogen - binding protein - containing fractions retained its capacity to completely transform to the
5 - S estrogen - binding protein . -
pubmed
In samples subjected to partial purification procedures , it was necessary that the buffer contain 40 mM
Tris , 60 mM
Tris , 60 mM
KC1 , 1 - 10 MM
dithiothreitol , and 1 M
urea at pH 7.4 , in order to accomplish the
4 - S to
5 - S estrogen - binding protein transformation at 25 degrees C. Formation of the
5 - S estrogen - binding protein requires association of the
4 - Estrogen - binding protein with a molecule identical to or very similar to itself . -
pubmed
Another population ,
T2 , is long - lived after
ATx , but highly sensitive to elimination by small doses of
ATS . -
pubmed
From precursors within the
T2 population , effectors of specific helper activity , after priming with
antigen , appeared within 1 to 2 days and reached a maximum on day 4 . -
pubmed
[ Detection of anti - idiotypic antibody specific for anti -
HBs in
HBsAg positive serum ] . -
pubmed
An ELISA for detecting anti - idiotypic antibody specific for anti -
HBs(anti - anti -
HBs , Ab 2 ) was devised and used to detect twenty - three asymptomatic
HBsAg positive sera . -
pubmed
Of these
HBsAg positive samples , five were positive for anti - anti -
HBs . -
pubmed
The authors suggested that samples with
HBsAg positive , especially those from asymptomatic carriers should be detected for
anti - anti - HBs in order to rule out the interference of
anti - anti - HBs . -
pubmed
The aberrant
PML proteins lacked the C terminus , which had been replaced by from two to ten
amino acid residues from the
RAR alpha sequence . -
pubmed
Findings supported the idea that endogenous
lectins could interact with
sugar residues and perform several roles including packaging , immobilization and transport of glucidic material . -
pubmed
Plasma
parathormone (
PTH ) and urinary
cyclic AMP ( ur . -
pubmed
Response was assessed by
barium swallow ,
CT scan , and measurement of
metastases where present . -
pubmed
In this study we have examined the effects of the
diterpene forskolin on various parameters of
B cell activation . -
pubmed
Cells were activated either by antibodies to
surface immunoglobulins (
anti - mu ) , by the
monoclonal antibody 1F5 reactive with the
CD20 antigen or by
12 - O - tetradecanoylphorbol 13 - acetate . -
pubmed
While
anti - mu stimulation induces increased
phosphatidylinositol (
PI ) turnover and [
Ca2+]i fluxes , the latter two reagents confer an activation of B cells independent of the
PI /
Ca2 + pathway . -
pubmed
We found a clear inhibitory effect of
forskolin on the anti - mu - induced
PI turnover and [
Ca2+]i fluxes as well as on later parameters of cell activation . -
pubmed
There was also a clear inhibition of
G1 entry and DNA synthesis when
PI /
Ca2 + -independent activation was employed , indicating that
cAMP interferes with B lymphocyte stimulation in several ways . -
pubmed
Importantly ,
forskolin maintained its inhibitory effect when added late after
anti - mu stimulation , implying an effect also at multiple stages of activation . -
pubmed
In the present study , we examine regulation of the isoenzymes by
thyroid hormone , which is well known to increase activity of
Na +- K +- ATPase in different tissue preparations . -
pubmed
The number of alpha(+)- and alpha - subunits in heart homogenates estimated by [
3H]
ouabain binding was 3.5 + /- 0.1 and 2.5 + /- 0.1 pmol / mg
protein , respectively [ alpha(+)/alpha = 1.40 ] . -
pubmed
Capillary blood
glucose concentrations before breakfast ( 5.2 + /- 0.4 mmol / l vs 9.1 + /- 0.8 mmol / l ) , after lunch ( 6.5 mmol / l + /- 0.8 vs 7.9 + /- 1.0 mmol / l ) and before the evening meal ( 5.0 + /- 0.7 mmol / l vs 7.7 + /- 0.7 mmol / l ) were lower on CSII , as were 24 - hour urine
glucose excretion and total
insulin dose ( 39.3 + /- 2.2 vs 49.8 + /- 4.0 U / day ) . -
pubmed
There was a significant positive correlation between fasting blood
glucose values and
glycosylated haemoglobin on injection but not pump treatment . -
pubmed
Its action on the
cAMP - dependent protein kinase was found to be competitive with regard to protein substrate , but noncompetitive with regard to
ATP and
cAMP . -
pubmed
For that reason , positive
post - narcotic
physostigmine effects could be due to its anti - opioid potency as well . -
pubmed
The organism has been categorized into 10 biotypes based on the variable fermentation of dextrin ,
maltose ,
mannitol , and
xylose and into 3 serotypes on the basis of heat stable ,
cell surface antigens . -
pubmed
[ On the effect of
trypsin ,
chymotrypsinogen ,
chymotrypsin , and
amylase on
iron resorption ] . -
pubmed
[
Adrergic beta - receptor blocking with
propranolol in some
disorders of cardiac rhythm ] . -
pubmed
While the demonstration of the in vivo inhibitory activity of
n - butyl malonate and
hydroxycitrate is entirely by physiological methods , that of the
transaminase inhibitors
aminooxyacetate and
cycloserine depends in part on the isolation of mutants capable of growth on
glycerol in minimal medium . -
pubmed
On this medium these mutants depend on the
malate -
aspartate shuttle for growth , and as expected the
transaminase inhibitors prevent their growth . -
pubmed
Asparagine - linked
glycopeptides were found to be mainly composed of the complex bi - antennary type as shown by affinity chromatography on concanavalin - A Sepharose ; high - molecular - weight
glycopeptides were represented by
nonfucosylated lactosaminoglycans since they were metabolically labeled with
[ 14C ] glucosamine but not with
[ 3H ] fucose , did not bind to
DEAE - cellulose , and were susceptible to
endo - beta - galactosidase . -
pubmed
Labeling with
galactose oxidase /
Na B3H4 technique demonstrated that lactosaminoglycans were present on the surface of differentiating germ cells and of testicular and epididymal spermatozoa . -
pubmed
On the other hand , epididymal spermatozoa lactosaminoglycans bound to the
lectin and therefore contained
fucose . -
pubmed
A soluble
fucosyltransferase , capable of transferring
fucose to germ cell lactosaminoglycans , was found to be present in the epididymis but not in the testis . -
pubmed
We suggest that these
molecules are fucosylated in vivo by a
fucosyltransferase secreted by the epididymal epithelium . -
pubmed
Erythropoiesis was quantitated by reticulocyte response , ferrokinetics ( plasma
iron turnover and marrow transit time ) , and by
hemoglobin C synthesis . -
pubmed
The
sarcoplasmic reticulum Ca ( 2 +)- ATPase loses hydrolytic activity and the ability to be phosphorylated by Pi following incubation with
EDC [
1 - ethyl-3 -( 3 - dimethylaminopropyl ) carbodiimide ] . -
pubmed
4 nmol of
tempamine per mg
SR protein can be coupled to either a
glu or an
asp side chain through the
EDC reaction . -
pubmed
In multivariate analysis ,
VLDL and global coronary
atherosclerosis score predicted
reinfarction ; plasma
plasminogen activator inhibitor-1 (
PAI-1 ) activity and global
coronary stenosis score predicted
cardiac death ; and
VLDL triglyceride levels , global coronary
atherosclerosis score and age predicted any major coronary event . -
pubmed
The
oxygen - binding binuclear center of
cytochrome bo is located in subunit I and consists of a
heme (
heme o ;
heme a3 in the
aa3 - type oxidases ) and a
copper (
Cu ( B ) ) . -
pubmed
Three other
amino acid residues which are potential ligands for
heme o are shown
tob e nonessential for enzyme activity . -
pubmed
These glycans can be released by treatment with
endoglycosidase H , and the majority has the same size as
Man5GlcNAc1 glycans . -
pubmed
thaliana with a transgenic
strain that expresses the
glycoprotein phytohemagglutinin , we obtained a unique
strain that synthesizes
phytohemagglutinin with two high -
mannose glycans , instead of one high -
mannose and one complex
glycan . -
pubmed
Interaction of
myosin with charged membranes is affected by
ATP . -
pubmed
Total
lysophosphatidylcholine concentration ( sum of
lysophosphatidylcholine remaining in tissue and
lysophosphatidylcholine released into the buffer ) increased from 0.73 + /- 0.08 nmol / mg protein at baseline to 1.83 + /- 0.45 nmol / mg protein after 5 minutes of
ischemia ( p & lt ; 0.001 ) , and was associated with evidence of cell
injury ( 26 % depletion of tissue
lactate dehydrogenase ) . -
pubmed
In contrast , there was no
lysophosphatidylcholine accumulation or release and no
lactate dehydrogenase depletion in oxygenated and perfused controls . -
pubmed
Attenuation of
lysophosphatidylcholine accumulation by incubation with
lysophospholipase did not prevent cell
injury . -
pubmed
This database indicated that groundwater concentrations of
TCE typically exceeded EPA 's
MCL of 5 ppb for
TCE . -
pubmed
The presence of
longitudinal sinus thrombosis was suggested by unenhanced
CT scan , confirmed by colour doppler flow imaging and magnetic resonance angiography , with absence of blood flow in the longitudinal
sinus . -
pubmed
Both prenatal
halothane and
TFAA exposure produced changes in liver biochemistry of newborns , as indicated by significant increases in the serum activities of
glutamate dehydrogenase and
aspartate aminotransferase . -
pubmed
In addition ,
TFAA caused a functional deficit of the proximal tubule in newborns , as evidenced by the significant increase in the urinary excretion of
beta 2 - microglobulin . -
pubmed
As a group ( n = 56 ) ,
RAR activity increased from a baseline of 1.2 + /- 0.2 to a peak of 4.3 + /- 0.3 impulses / breath at 8 - 10 min after the onset of
PAE induction . -
pubmed
Studies were repeated in 33
RARs initially stimulated by
PAE . -
pubmed
In another
24 receptors , the responses of
RARs to
PAE were unaltered by pretreatment with saline ( n = 8) or
dimethylthiourea ( a
hydroxyl radical scavenger ; n = 8) , but was significantly attenuated by pretreatment with
ibuprofen ( a
cyclooxygenase inhibitor : n = 8) . -
pubmed
These results suggest that : ( 1 ) the increased
RAR activity following
PAE is associated primarily with an increase in bronchomotor tone and secondarily with the mechanical action of the beating heart impacting on lung tissues ; and ( 2 )
cyclooxygenase products may be involved in this sensory stimulation . -
pubmed
Adenosine receptor blockade with
8 - phenyltheophylline (
8 - PT ) was used to shift the
adenosine dose - response curve 12 - fold . -
pubmed
During intracoronary
norepinephrine infusion , coronary blood flow and myocardial
oxygen consumption increased similarly before and after 8 - PT , demonstrating a lack of an effect from the
adenosine receptor blockade . -
pubmed
After 8 - PT , a similar increase in estimated interstitial
adenosine was found , demonstrating that there was no augmentation in
adenosine concentration to overcome the
adenosine receptor blockade . -
pubmed
Thus
adenosine could not be responsible for the increase in coronary blood flow after
adenosine receptor blockade and therefore is not required for
norepinephrine - induced
hyperemia . -
pubmed
This was demonstrated with the use of blocking antibodies directed to
alpha4beta1 and peptide adhesion competitors containing the alpha4beta1 binding
tripeptide ,
Leu - Asp - Val ( LDV ) . -
pubmed
Consistent with this data , the adhesion is completely divalent cation - dependent and is stimulated by known activators of
leukocyte integrin function , namely
phorbol ester and the
beta1 integrin activating antibody ,
8A2 . -
pubmed
The pH dependence of the model results from the binding of
hydrogen ions to
TM macromolecules -- to both neutral sites with basic pK and negatively charged sites with acidic pK. -
pubmed
The exposure of the
tyrosyl component is less in
apo - TL than in
holo - TL . -
pubmed
The sole
tryptophan residue ,
Trp17 , is buried in both
holo - and apo - TL . -
pubmed
The steady state exposure of
Trp17 is the same in holo- and
apo - TL , but the dynamic exposure is two - fold greater in
apo - TL . -
pubmed
Maneuvers to unfold the
protein with
urea or incubation in an acidic environment resulted in increased exposure of
aromatic amino acids . -
pubmed
Meta - analysis of
streptokinase and
heparin in
deep vein thrombosis . -
pubmed
The efficacy and safety of
streptokinase and
heparin in
deep vein thrombosis (
DVT ) were compared in a meta - analysis . -
pubmed
Randomized trials in which
streptokinase ( followed by
heparin ) and
heparin alone were compared in treating phlebographically documented acute
DVT were identified from MEDLINE and other sources for a meta - analysis . -
pubmed
A fixed - effect model was used to combine the study results , enabling differences between
streptokinase recipients and recipients of
heparin alone to be expressed as summary ORs with 95 % CIs . -
pubmed
Significantly more
streptokinase recipients achieved
thrombolysis than recipients of
heparin alone ( summary OR , 6.24 ; 95 % CI , 3.62 to 10.78 ) . -
pubmed
The frequency of major
bleeding was significantly higher among
streptokinase recipients than recipients of
heparin alone ( summary OR , 3.78 ; 95 % CI , 1.26 to 11.32 ) . -
pubmed
A meta - analysis showed that , compared with
heparin alone ,
streptokinase therapy for
DVT was associated with significantly more frequent
thrombolysis and major
bleeding ; after exclusion of one outlying study , analysis showed that
streptokinase therapy was associated less frequently than
heparin alone with postthrombotic changes ( assessed by phlebographic evaluation ) and
postphlebitic syndrome . -
pubmed
P450BM-3 : absolute configuration of the primary metabolites of
palmitic acid . -
pubmed
P450BM-3 catalyzes the oxidation of short- and long - chain ,
saturated and unsaturated fatty acids . -
pubmed
While the
15 - and 14 - hydroxy compounds are produced in a highly stereoselective manner ( 98 % R , 2 % S ) , the
13 - hydroxy is a mixture of 72 % R and 28 % S. We have also examined the binding of these three
hydroxy acids to
P450BM-3 and shown that only two of them (
14 - OH and
13 - OH palmitic acid ) can bind to and be further metabolized by
P450BM-3 . -
pubmed
The results indicate that in contrast to the flexibility of
palmitoleic acid bound to the oxidized
enzyme ,
palmitic acid is rigidly bound in the active site during catalytic turnover . -
pubmed
All major classes of antihypertensive agents (
calcium channel blockers , diuretics , beta - blockers ,
angiotensin - converting enzyme inhibitors ) can cause
LVH regression but not all to the same degree . -
pubmed
Angiotensin - converting enzyme inhibitors may provide the most pronounced reduction in left ventricular mass per millimeter of
mercury of blood pressure reduction . -
pubmed
Because ARBs act specifically on the
AT ( 1 ) receptor ,
angiotensin II can exert its favorable effects on cell growth inhibition through the
AT ( 2 ) receptor . -
pubmed
coli lacking endogenous
cysteine synthase genes could functionally rescue the growth without addition of
cysteine . -
pubmed
The activity of recombinant
serine acetyltransferase produced from the cDNA was inhibited by
L - cysteine , which is the end - product of the pathway ; however , the sensitivity to
cysteine ( 48.7 microM of the concentration for 50 % inhibition , IC(50 ) ) was fairly low compared with that of previously reported
serine acetyltransferases ( approximately 5 microM IC(50 ) ) from various plants . -
pubmed
tuberosum is likely due to the lower sensitivity of feedback inhibition of
serine acetyltransferase to
cysteine . -
pubmed
The analysis of the predicted
amino acid sequence of the cDNA clone revealed that the encoded protein contains two RNA binding domains , including the conserved consensus ribonucleoprotein sequences
CS - RNP1 and
CS - RNP2 , on the C - terminus half and the presence of a putative transit peptide sequence in the N - terminus region . -
pubmed
17betaE(2 ) and
bio-17betaE(2 ) , but not other hormones , were correlated with
BMD after adjustment for age and
body weight . -
pubmed
Based on previous studies , unfiltered
sulfur colloid provided a higher success rate of
SN identification than the other tracer types . -
pubmed
Further evaluation with
99mTc - sulfur colloid demonstrated that increased volume increased the success rate of
SN identification . -
pubmed
Electrophoretic mobility shift experiments with primary Th ( T helper ) cells showed the formation of a specific ,
T - cell receptor - inducible complex at this site that is sensitive to
cyclosporin A and supershifted with
anti - NFATc2 in both Th1 and Th2 cells . -
pubmed
This toxin , named
OsK2 , has been characterized as a 28 - residue peptide , containing six conserved
cysteine residues and was shown to be a potent and selective blocker of
Kv1.2 channels ( K(d ) = 97 nM ) . -
pubmed
OsK2 is the second member of the 13th subfamily of short - chain
K (+ ) channel - blocking peptides known thus far and is therefore called
alpha - KTx 13.2 . -
pubmed
( D'Arcy ) secrete large amounts of
2,3,4 - tri - O - acylglucoses possessing straight- and branched - chain
fatty acids of short to medium chain length (
C4 - C12 ) . -
pubmed
These fused - ring
polyketides are formed by the action of
polyketide synthases (
PKSs ) , which catalyse the assembly , folding and cross - linking of
poly - beta - ketoacyl intermediates . -
pubmed
Adenosine or the selective
A1 receptor agonist
R - PIA causes a concentration - dependent contraction . -
pubmed
After pretreatment with
A1 receptor antagonist ,
DPCPX ,
adenosine - mediated contraction was abolished . -
pubmed
Adenosine - induced contraction was significantly increased when
A1 receptors were preserved by pretreatment with
DPCPX followed by inactivation of all unprotected receptors with
N - ethylmaleimide . -
pubmed
Adenosine- or
R - PIA - induced contraction was significantly augmented in the preserved cells and the increase was abolished in the presence of the
A1 receptor antagonist
DPCPX . -
pubmed
PTX abolished contraction induced by
adenosine or
R - PIA , implying that contraction activated by
A1 receptor was coupled to a
pertussis toxin (
PTX)-sensitive
G ( i ) protein . -
pubmed
Adenosine- or
R - PIA - induced contraction of esophageal smooth muscle cells was not affected by the
phospholipase D (
PLD ) inhibitor
rho - chloromercuribenzoic acid (
rhoCMB ) ,
phospholipase A ( 2 ) (
PLA ( 2 ) ) inhibitor
DEDA or
PKC antagonist
chelerythrine , but was significantly abolished by
phospholipase C ( PLC ) inhibitor ,
neomycin . -
pubmed
PLC - beta3 antibody inhibited
R - PIA - induced contraction . -
pubmed
R - PIA - induced contraction of esophageal muscle cells was inhibited by
IP ( 3 ) receptor antagonist
heparin , which suggests that the contraction of esophageal smooth muscle cells is dependent on
phosphatidylinositol - specific phospholipase (
PI - PLC ) and
IP ( 3 ) . -
pubmed
A1 receptor is coupled to
PTX - sensitive
G protein G ( i2 ) , which results in the activation of
PI - PLC - beta3 . -
pubmed
PI hydrolysis by
PI - PLC forms IP(3 ) , which binds to
IP ( 3 ) receptor on endoplasmic reticulum , resulting in the release of intracellular
Ca ( 2 + ) . -
pubmed
A reversed - phase liquid chromatography ( LC ) method has been developed , optimised and validated for the separation and quantitation of
capsaicin (
CP ) and
dihydrocapsaicin (
DHCP ) in a topical cream formulation . -
pubmed
The conditions allow the separation and quantitation of
CP and
DHCP without interference from the other substances contained in the cream . -
pubmed
A
technetium 99m - labeled tetrofosmin stress -
rest tomographic scan and
iodine 123 - labeled metaiodobenzylguanidine planar scans were performed before and after transmyocardial laser revascularization ( 3 and 12 months later ) to evaluate myocardial perfusion and innervation . -
pubmed
Liver and muscle tissues were isolated from exposed , recovery and control fish at the end of 1 , 3 and 6 days in each condition , and used for the estimation of protein ,
amino acids ,
ammonia , glycogen ,
aminotransaminases ,
glycogen phosphorylase ( a and
ab ) and
glutamate dehydrogenase . -
pubmed
The
glycogen phosphorylase ab activity in liver was suppressed on exposure to
carbofuran . -
pubmed
Significantly , specific inhibition of
GSK-3 activity by
LiCl prevents hyperphosphorylation of
tau , and spatial
memory impairment resulting from
PI3 K and
PKC inhibition . -
pubmed
Thyroid hormone induces
erythropoietin gene expression through augmented accumulation of
hypoxia - inducible factor-1 . -
pubmed
Hypoxia , the reduction of
oxygen supply , results in adaptationally appropriate alterations in gene expression through the activation of
hypoxia - inducible factor 1 (
HIF-1 ) to overcome any shortage of
oxygen . -
pubmed
Thyroid hormones have been found to augment the
oxygen capacity of the blood by increasing the production of
erythropoietin (
EPO ) and to improve perfusion by vasodilation through the augmented expression of
adrenomedullin (
ADM ) . -
pubmed
Knocking down
HIF-1alpha by short interfering RNA ( siRNA ) clearly demonstrated that
thyroid hormone - induced target gene expression required the presence of
HIF-1 . -
pubmed
Although an increased association of the two known coactivators of
HIF-1 ,
p300 and
SRC-1 , was found ,
thyroid hormone did not affect the activity of the isolated
COOH - terminal transactivating domain of
HIF-1alpha . -
pubmed
Increased synthesis of
HIF-1alpha may contribute to the adaptive response of increased
oxygen demand under
hyperthyroid conditions . -
pubmed
The
calcium / calmodulin - dependent protein kinases (
CaM kinases ) are a family of related
kinases that are activated in response to increased intracellular
calcium concentrations . -
pubmed
Surprisingly , this activation occurs in the absence of any detectable
calcium flux , suggesting a novel means for the activation of these
kinases . -
pubmed
The addition of
catalase to the cultures inhibited
PMA - induced activation of the
CaM kinases , suggesting that similar to
hydrogen peroxide ,
PMA also activates the
CaM kinases via the production of ROI . -
pubmed
In support of this concept ,
okadaic acid and
microcystin - LR , which are inhibitors of
protein phosphatase 2A (
PP2A ) , induced
CaM kinase II and IV activity in these cells . -
pubmed
A synthetic
p38 alpha MAP kinase inhibitor ,
SD-282 ( 40 mg / kg ) or vehicle ( 0.25 %
Tween 80 in saline ) was given intraperitoneally twice a day for 3 days , concomitant with
ISO treatment . -
pubmed
The
ISO - induced expression of
COX-2 ,
collagen I and III , and
fibronectin genes was reduced significantly ( P & lt ; 0.05 in all cases ) by administration of
SD-282 . -
pubmed
Furthermore ,
dopamine modulated the circadian rhythms in expression of
LC opsin mRNA . -
pubmed
Administration of
dopamine increased
LC opsin mRNA expression , but only in the early morning . -
pubmed
Effect of
DNA polymerase beta loop variants on discrimination of
O6 - methyldeoxyguanosine modification present in the
nucleotide versus template substrate . -
pubmed
We have examined the mechanism of
DNA polymerase beta (
pol beta ) lesion discrimination using
alkylated dNTP versus alkylated DNA template substrates and the
pol beta variants R253 M and E249K. -
pubmed
Both of these
amino acid variants are located in the loop region of the
palm domain and are known to play a role in
pol beta fidelity and discrimination of
3 '- azido-3 '- deoxythymidine triphosphate substrates . -
pubmed
Under steady - state conditions , the ratio of inherent reactivity values for the m6dGTP substrate relative to the
dGTP substrate was greater for both variant
polymerases than for wild - type ( WT )
pol beta . -
pubmed
Using
N - methyl - N - nitrosourea- (
MNU- ) modified DNA templates in the HSV - tk in vitro assay , no difference among the
enzymes in the frequency of alkylation - induced G to A transition mutations was observed . -
pubmed
We conclude that a specific interaction at the loop of the
palm domain is involved in
pol beta discrimination of the m6 G lesion when present on the incoming
dNTP substrate but not when present in the DNA template . -
pubmed
Neither
protein kinase C- nor
protein tyrosine kinase - mediated pathways were involved in regulating
riboflavin uptake . -
pubmed
The system appears to be regulated by
protein kinase A and
Ca2 +/calmodulin pathways . -
pubmed
Mutant genes giving rise to high
GFP fluorescence intensity indicating high levels of soluble TEV - GFP were subsequently transferred to a vector providing a C - terminal
histidine tag for expression , purification , and activity tests of mutated TEV . -
pubmed
We identified a mutant , TEV(SH ) , in which three
amino acid substitutions result in a five - fold increase in the yield of purified
protease with retained activity . -
pubmed
The substrate specificity of
SlrA is typical for prokaryotic and eukaryotic
cyclophilins , with
Suc - Ala - Ala - Pro - Phe - p - nitroanilide (
pNA ) being the most rapidly catalyzed substrate . -
pubmed
Vitamin D in
chronic kidney disease : a systemic role for selective
vitamin D receptor activation . -
pubmed
Optimization of
2,4 - diaminopyrimidines as
GHS - R antagonists : side chain exploration . -
pubmed
The synthesis and structure - activity relationships of the 4- and 6 - substituents of
2,4 - diaminopyrimidine - based
growth hormone secretagogue receptor (
GHS - R ) antagonists are described . -
pubmed
Diaminopyrimidines with
6 - norbornenyl ( 4n ) and
6 - tetrahydrofuranyl ( 4p ) substitutents were found to exhibit potent
GHS - R antagonism and good selectivity ( approximately 1000 - fold ) against
dihydrofolate reductase . -
pubmed
To elucidate why DH , an agonist , and
MSVIII-19 , a competitive antagonist , bind selectively to
glutamate receptor ( GluR ) 5 but not to the
KA2 KAR subunit , we used molecular dynamics simulations to generate binding models that were tested experimentally in radioligand binding and electrophysiological assays . -
pubmed
Three candidate sites ,
Val685 ,
Leu735 , and
Ser741 in
GluR5 , corresponding to
Ile669 ,
Phe719 , and
Met725 in
KA2 , were predicted to underlie the distinct binding profiles of the marine toxins . -
pubmed
Most notably , mutation of
Met725 to
serine in
KA2 increased the affinity of DH by 350 - fold ; in contrast , mutation of one or more of the residues in
GluR5 did not markedly alter DH binding . -
pubmed
In the present contribution , we address the problem of the nuclearity of the
iron species in
Fe - ZSM-5 by a combination of techniques , that is , UV - Vis , EXAFS , Magnetic Circular Dichroism (
MCD ) and magnetisation measurements . -
pubmed
Propofol reduces
pulmonary edema by prevention of
cytokine - mediated impairment of alveolar ion and fluid transport . -
pubmed
The pellet remaining after
SDS extraction was also treated with
trypsin . -
pubmed
AIM : The aim of this study was to evaluate the maxillary
sinus growth and health in the presence and absence of postnasal air flow through a unique model . -
pubmed
In contrast ,
phospho -
Akt was down - regulated to the same extent 2 h after acute
AMPH or repeated
AMPH with an
AMPH challenge . -
pubmed
These data implicate differential regulation of
phospho - extracellular signal - regulated kinase ,
phospho - cAMP response element - binding protein versus
phospho -
Akt in sensitized responses to
AMPH . -
pubmed
PGE2 inhibits
apical K channels in the CCD through activation of the
MAPK pathway . -
pubmed
We used the patch -
clamp technique and Western blot analysis to explore the effect of
PGE ( 2 ) on
ROMK - like small - conductance K ( SK ) channels and
Ca ( 2 +)-activated big - conductance K channels (
BK ) in the cortical collecting duct ( CCD ) . -
pubmed
Application of 10 microM
PGE ( 2 ) inhibited
SK and BK channels in the CCD . -
pubmed
Moreover , either inhibition of
PKC or blocking
mitogen - activated protein kinase (
MAPK ) ,
P38 and
ERK , abolished the effect of
PGE ( 2 ) on SK channels in the CCD . -
pubmed
The effect of
PGE ( 2 ) on SK channels was completely blocked in the presence of
SC-51089 , a specific
EP1 receptor antagonist , and mimicked by application of
sulprostone , an agonist for
EP1 and
EP3 receptors . -
pubmed
Application of
PGE ( 2 ) significantly stimulated the phosphorylation of
P38 and
ERK within 5 min . -
pubmed
The dose - response curve of
PGE ( 2 ) effect shows that 1 , 5 , and 10 microM
PGE ( 2 ) increased the phosphorylation of
P38 and
ERK by 20 - 21 , 50 - 80 , and 80 - 100 % , respectively . -
pubmed
The stimulatory effect of
PGE ( 2 ) on
MAPK phosphorylation was not affected by
indomethacin but abolished by inhibition of
PKC . -
pubmed
This suggests that the effect of
PGE ( 2 ) on
MAPK phosphorylation is
PKC dependent . -
pubmed
We conclude that
PGE ( 2 ) inhibits SK and BK channels and that there is an effect of
PGE ( 2 ) on SK channels in the CCD through activation of
EP1 receptor and
MAPK pathways . -
pubmed
Also , high concentrations of
PGE ( 2 ) induced by K restriction may be partially responsible for increasing
MAPK activity during K restriction . -
pubmed
On the basis of these analytical results , we propose a pathway for
dioxane oxidation by
monooxygenase - expressing cells in which
dioxane is first converted to
2 - hydroxy-1,4 - dioxane , which is spontaneously oxidized to
HEAA . -
pubmed
(
bevacizumab ) , which is reactive with all
VEGF - A isoforms , was radiolabeled with In-111 or with I-125 . -
pubmed
To develop potent and highly selective
transthyretin (
TTR ) amyloidogenesis inhibitors , it is useful to systematically optimize the three substructural elements that compose a typical
TTR kinetic stabilizer : the two
aryl rings and the linker joining them . -
pubmed
Coconsideration of amyloid inhibition and ex vivo plasma
TTR binding selectivity data reveal that direct connection of the two
aryls or linkage through nonpolar
E - olefin or -
CH 2CH 2- substructures generates the most potent and selective
TTR amyloidogenesis inhibitors exhibiting minimal undesirable binding to the
thyroid hormone nuclear receptor or the
COX-1 enzyme . -
pubmed
Here , we show that hippocampal
CA1 pyramidal cell synapses differ in size , shape , and
glutamate receptor expression depending on the laterality of presynaptic origin . -
pubmed
Acromegaly : correlation between expression of
somatostatin receptor subtypes and response to
octreotide - lar treatment . -
pubmed
About one - third of
acromegalics are resistant to the clinically available
somatostatin analogs (
SA ) . -
pubmed
N ( G )- hydroxy - L - arginine , a putative intermediate in the
NOS - reaction , gave no
NO emission . -
pubmed
Production of
NO was decreased by
anoxia or by the addition of
catalase , but was increased by conditions inducing reactive
oxygen ( ROS ) or by the addition of
hydrogen peroxide . -
pubmed
Cell - free
enzyme solutions generating
superoxide or
hydrogen peroxide also led to the formation of
NO from HA or ( with lower rates ) from SHAM , and
nitrite was also an oxidation product . -
pubmed
Unexpectedly , the addition of
superoxide dismutase (
SOD ) to cell suspensions stimulated
NO formation from
hydroxylamines , and
SOD alone ( without cells ) also catalysed the production of
NO from HA or SHAM . -
pubmed
NO production by
SOD plus HA was higher in
nitrogen than in air , but from
SOD plus
SHAM it was lower in
nitrogen . -
pubmed
Thus ,
SOD - catalysed
NO formation from
SHAM and from HA may involve different mechanisms . -
pubmed
BACKGROUND : In this study , levels and rates of change in total
testosterone ( T ) ,
sex hormone - binding globulin (
SHBG ) and free
androgen index ( FAI ) were related to chronological age and to the final menstrual period ( FMP ) as an indicator of ovarian aging . -
pubmed
Huntington ' s disease (
HD ) is an
autosomal dominantly inherited disorder , caused by an expanded
polyglutamine region of a protein called
huntingtin . -
pubmed
Their early rise in intensity , occurring within few minutes after the
alcohol injection , emphasizes how susceptible
troponin bands are to chemical /
ischemic insults . -
pubmed
The inhibitor is a stable
deoxy analogue of the tetrahedral intermediate presumed to form in the
NeuB reaction and was synthesized as a mixture of stereoisomers at the key tetrahedral center . -
pubmed
Second , it serves as a source of nucleophilic water and delivers it to the si
face of the
oxocarbenium intermediate to generate a tetrahedral intermediate with a ( 2R)-configuration . -
pubmed
This specificity is well explained by the very hydrophobic nature of the
S1 ' subsite that consists of
Tyr(189 ) ,
Ile(213 ) ,
Ile(300 ) ,
Met(289 ) ,
Val /
Leu(291 ) and
Leu(298 ) . -
pubmed
The dual characteristics of
chymosin are due to the occurrence of polar / charged residues in the
S1 ' subsite , such as
Glu /
Asp(289 ) ,
Gln(298 ) and
Lys /
Gln(299 ) , which are different from the S1 ' subsite of
pepsin A. -
pubmed
We aimed to determine the value of sidestream end - tidal
carbon dioxide (
SS - ETCO2 ) measurement in patients with
chronic obstructive pulmonary disease (
COPD ) in the emergency department . -
pubmed
We also studied correlations between
Se and other biochemical variables ( age , CSF protein concentrations and
glutathione peroxidase activity and plasma
Se values ) . -
pubmed
No association was observed between plasma and
CSF Se concentrations . -
pubmed
Median
CSF Se values were 32 times lower when compared with those for plasma . -
pubmed
In conclusion ,
CSF Se concentrations depend on age and total
CSF protein values . -
pubmed
The association observed between
CSF Se and
GPX activity suggests that
Se quantification might be a reflection of some
Se - dependent protein function . -
pubmed
1 - Aminobenzotriazole (
ABT ) has been widely used in drug development process as an irreversible inhibitor of
CYP enzymes . -
pubmed
One potential use of
ABT is to potentiate pharmacological effects of rapidly - metabolized drugs on
CYP expression by inhibiting their metabolism ; however ,
ABT 's own effects on
CYP expression have been unknown . -
pubmed
In this study , we show that
ABT up - regulates expression of
CYP2B6 and
CYP3A4 potentially by activating nuclear receptor
CAR . -
pubmed
ABT also modulated
CYP - inducing actions of
CITCO and
rifampin , the known inducers of
CYP2B6 and
CYP3A4 . -
pubmed
These results suggest that the use of
ABT as a potentiator of pharmacological effects of rapidly - metabolized drugs is limited due to its own pharmacological actions on
CYP expression as a
CAR activator . -
pubmed
The elastic biomaterial is chemically cross - linked via interaction between
primary amine groups naturally present on the two proteins , silk and
elastin , or chemically introduced on hyaluronan and
N - succinimide functionalities of the cross - linker . -
pubmed
BACKGROUND : The
copper ( Cu ) transporter ( COPT / Ctr ) gene family has an important role in the maintenance of
Cu homeostasis in different species . -
pubmed
However , only two ,
COPT1 and
COPT5 , have been characterized for their functions in
Cu transport . -
pubmed
However , these
COPT genes , which showed different tissue - specific expression patterns and
Cu level - regulated expression patterns , were also transcriptionally influenced by deficiency of
iron ,
manganese , or
zinc . -
pubmed
Tight
glucose control ( TGC ) using a sliding scale based on intermittent blood
glucose measurements occasionally can have a fatal outcome as a result of
insulin - induced
hypoglycemia . -
pubmed
The mean blood
glucose level was 129.7 + - 9.7 mg / dl in the TGC group and 200.9 + - 14.7 mg / dl in the
GC group ( P & lt ; 0.01 , ANOVA ) . -
pubmed
Histologic analysis from group 2 showed that , compared with placebo treatment ,
collagen organization in
avotermin - treated
scars more closely resembled normal skin in 14 of 19 cases . -
pubmed
We have recently performed the immune - LCM of
formaldehyde - fixated ,
paraffin - embedded tissues immunostained with a monoclonal antibody
Factor VIII . -
pubmed
CD300f also binds
PMA /
ionomycin - activated splenocytes and Ag - stimulated T cells , an interaction inhibited by
Annexin V. By ELISA , cosedimentation , and surface plasmon resonance using phospholipid - containing liposomes , we show that
CD300f preferentially binds
phosphatidylserine and requires a metal ion . -
pubmed
We conclude that expression of
CD300f conveys additional capacity to recognize
phosphatidylserine to myeloid cells . -
pubmed
Early graft function was defined as
IGF ( N=17 ) when serum
creatinine ( sCr ) was & lt;264 micromol / l at 3(rd ) postoperative day ( POD ) ; as
SGF ( N=20 ) when sCr & gt;264 micromol / l and not more than 1 dialysis was performed ; and as DGF ( N=19 ) when more than 1 dialysis was done . -
pubmed
The research challenge for this detector is implementing effective detection of scintillation photons by covering six
faces of a segmented crystal block with
silicon photomultipliers ( SiPMs ) . -
pubmed
An inactive
t - PA variant inhibited the
t - PA - mediated increase in permeability , whereas blockade of
low - density lipoprotein receptors or exposed
lysine residues resulted in similar inhibition , implying a role for both a
t - PA receptor , most likely a
low - density lipoprotein receptor , and a
plasminogen receptor . -
pubmed
SNP induced
nephrotoxicity by increasing serum
creatinine and blood
urea nitrogen ,
SNP significantly decreased
malate dehydrogenase ,
glucose-6 - phosphatase ,
fructose-1,6 - bisphosphatase and malic enzyme but increased
lactate dehydrogenase and
glucose-6 - phosphate dehydrogenase . -
pubmed
The activity of
catalase and
glutathione peroxidase decreased concomitantly with increased lipid peroxidation , indicating that the significant
kidney damage has been inflicted by
SNP . -
pubmed
Biomarkers of oxidative protein damage (
3 - nitrotyrosine ; 3 -
NT ) and oxidative DNA damage (
8 - oxo - deoxyguanosine ;
8 - oxo - dG ) were also assessed . -
pubmed
Functional indicators of oxidative stress included relative levels of
3 - chlorotyrosine (
3 - CT ) , an established biomarker of a chronic inflammatory response , and
aconitase activity , a biomarker of mitochondrial
superoxide production . -
pubmed
There was a significant increase in 3 - CT levels in both brain regions ( P<0.01 ) , whereas
aconitase activity was significantly decreased in
autism cerebellum ( P<0.01 ) , and was negatively correlated with
GSH /
GSSG ( P=0.01 ) . -
pubmed
Pre - exposure to
copper partially prevented the
mercury effect on liver but not on kidney
PBG synthase , and prevented the increase of the
creatinine levels . -
pubmed
Here we describe in - life and
post - mortem observations for a hypomorphic
Ercc1 variant ,
Ercc1(-/Delta7 ) , which is hemizygous for a single truncated
Ercc1 allele , encoding a protein lacking the last seven
amino acids . -
pubmed
Based on
amino acid sequence homology , hydrophobic cluster and three dimensional structure analyses , it was attested that the
xylanase belongs to the
glycoside hydrolase ( GH ) families 10 with five
carbohydrate binding domains . -
pubmed
thermarum is one of the hyperthermophilic
xylanases that exhibits high thermostability , and thus , is a suitable candidate for generating XOs from cellulosic materials such as agricultural and forestry residues for the uses as prebiotics and precursors for further preparation of
furfural and other chemicals . -
pubmed
The
SCN contains the body 's master '
clock ' while the pineal - SCN nexus is intricate to the nighttime production of
melatonin . -
pubmed
Through its actions on
melatonin receptors agomelatine can resynchronise circadian rhythms . -
pubmed
Exogenous
melatonin administration and
bright light therapy have mixed results in limited controlled clinical evaluations . -
pubmed
Furthermore ,
agomelatine has other actions which pre - clinical studies suggest are as important to its therapeutic effects as are its actions on
melatonin receptors ipso facto its resynchronising properties . -
pubmed
Maraviroc , a
CCR5 antagonist , blocks interactions between the
viral envelope proteins and the
CCR5 coreceptor . -
pubmed
Enfuvirtide , a fusion inhibitor , disrupts conformational changes in
gp41 that drive membrane fusion . -
pubmed
BACKGROUND : Our previous studies showed that hypothermic storage ( HS ) induces red blood cell ( RBC ) microparticle (
RMP ) generation and changes in
phosphatidylserine (
PS ) and
CD47 expression on RBCs and RMPs . -
pubmed
Units were assessed for
hemolysis ,
adenosine triphosphate (
ATP ) concentration , lipid composition , and
RMP generation , as well as PS and
CD47 expression throughout 49 days of HS . -
pubmed
Synthesis and in vitro
urease inhibitory activity of
N , N '- disubstituted thioureas . -
pubmed
Thiourea derivatives ( 1 - 38 ) were synthesized and evaluated for their
urease inhibition potential . -
pubmed
The synthetic compounds showed a varying degree of in vitro
urease inhibition with IC50 values 5.53 + - 0.02 - 91.50 + - 0.08 muM , most of which are superior to the standard
thiourea ( IC50 = 21.00 + - 0.11 muM ) . -
pubmed
Optical absorption and electron paramagnetic resonance (
EPR ) spectroscopy experiments reveal that red light induces the reduction of
perfluoroisopropyl - substituted zinc ( ii ) phthalocyanine (
F64PcZn ) dissolved in
ethanol . -
pubmed
The UV - induced triplet state of
F64PcZn is characterized using a combination of transient
EPR experiments and DFT computations . -
pubmed
METHODS : In the present work , we further analyzed the
peptidase expression in the midgut of
sugar - fed females using 1DE - substrate gel zymography , two - dimensional electrophoresis ( 2DE ) , mass spectrometry (
MS ) , and protein identification based on similarity . -
pubmed
Concomitant use of the fluorogenic substrate
Z - Phe - Arg - AMC and
trypsin - like
serine protease inhibitors corroborated the zymographic findings . -
pubmed
Furthermore , the use of
avidin -
biotin interaction allows highly selective pre- or
post - functionalization of the protein crystals in a modular way with different types of functional units , such as fluorescent dyes , enzymes and plasmonic nanoparticles . -
pubmed
The treatments analysed were : antihypertensive treatments , statins , antiplatelet drugs ,
xanthine oxidase inhibitors , correction of
metabolic acidosis , treatment with (
calcium or non -
calcium )
phosphate binders ,
vitamin D (
calcitriol or
paricalcitol ) ,
erythropoietin and the availability of an
internal arteriovenous fistula (
IAVF ) . -
pubmed
In the multivariate models , we observed that , in addition to age , the comorbidity index ,
serum albumin , pre - dialysis treatment with
angiotensin - converting - enzyme inhibitors and/or
angiotensin receptor blockers , correction of
acidosis with
sodium bicarbonate and IAVF at the start of haemodialysis were significantly associated with better survival in dialysis . -
pubmed
BACKGROUND AND OBJECTIVES :
Pegylated interferon (
peg - IFN )- alpha2a and - alpha2b show different pharmacokinetic properties but are used interchangeably for
hepatitis C treatment in traditional dual combinations and with newer agents . -
pubmed
Histology grading and staging ,
interleukin-28B (
IL28B ) status and baseline viral genotype ,
alanine aminotransferase ,
gamma glutamyltransferase and
glucose were analysed . -
pubmed
We identified
accD1 and
fasA as key genes of
fatty acid synthesis , encoding the beta - subunit of the
acetyl - CoA carboxylase and a
type - I fatty acid synthase , respectively , and observed their repression during growth on minimal medium with
acetate . -
pubmed
In the present work we establish by co - isolation and gel - mobility shifts
oleoyl - CoA and
palmitoyl - CoA as effectors of
FasR , and show by DNA microarray analysis that in presence of exogeneous
fatty acids accD1 and
fasA are repressed . -
pubmed
These results are evidence that
acyl - CoA derivatives derived from extracellular
fatty acids interact with
FasR to repress the genes of
fatty acid synthesis . -
pubmed
This model also explains the observed repression of
accD1 and
fasA during growth on
acetate , where apparently the known high intracellular
acetyl - CoA concentration during growth on this substrate requires reduced
accD1 and
fasA expression for fine control of de novo
fatty acid synthesis . -
pubmed
However , the genes specific to
mycolic acid synthesis , which are in part shared with linear
fatty acid synthesis , are not controlled by
FasR , which is in agreement with the fact that they can not be supplied from the extracellular environment but that their synthesis fully depends on a constant supply of linear
fatty acid chains . -
pubmed
The proportion of patients who died from
cancer was the highest in the
calcium channel blocker ( CCB ) group ( 6.5 % ) , followed by
thiazide diuretics ( 4.4 % ) ,
angiotensin converting enzyme inhibitors ( 4.2 % ) and beta - blockers ( 2.6 % ) . -
pubmed
Sphingosylphosphorylcholine potentiates vasoreactivity and voltage - gated
Ca2 + entry via
NOX1 and reactive
oxygen species . -
pubmed
The potentiation was greatly suppressed or abolished by inhibition of
phospholipase C (
PLC ;
U73122 ) ,
PKCepsilon (
inhibitory peptide ) ,
Src (
PP2 ) , and
NADPH oxidase (
VAS2870 ) , and also by
Tempol (
superoxide scavenger ) , but not by inhibition of
Rho kinase (
Y27632 ) . -
pubmed
SPC elevated reactive
oxygen species ( ROS ) in vascular smooth muscle cells , and this was blocked by
PP2 ,
VAS2870 , and siRNA knockdown of
PKCepsilon . -
pubmed
CONCLUSION : Our results suggest that low concentrations of
SPC activate a
PLC - coupled and
NOX1 - mediated increase in ROS , with consequent enhancement of voltage - gated
Ca2 + entry and thus vasoreactivity . -
pubmed
Results showed that the HRT of 2.7 days with
TM /
TP = 0.57 was the best to achieve simultaneous nutrients removal for the influent with initial soluble chemical
oxygen demand ( SCOD ) of about 2000 mg L(-1 ) ( only 0.55 mg L(-1 )
NH4 - N , & lt ; 0.1 mg L(-1 )
nitrate , and 0.14 mg L(-1 ) soluble
phosphorus in the effluent ) . -
pubmed
The diagnostic protocol consisted of 1 ) history of the reaction , 2 ) measurement of serum baseline
tryptase and specific
IgE - assays for latex ,
beta - lactams and
succinylcholine , 3 ) skin tests for the agents listed in the anaesthesia chart and for others likely to be safe for future use , latex , and others medications administered during the perioperative period ( i.e. -
pubmed
A diagnosis of
IgE - mediated reaction was established in 55 cases ( 28.50 % ) ; the most common causes were neuromuscular blocking agents , followed by latex and
beta - lactams . -
pubmed
The data from
11C - PIB PET scanning showed that
amyloid beta deposition in PCA was comparable to TAD . -
pubmed
Population - weighted , logistic models estimated how strongly each anthropometric indicator was associated with 5
cardiometabolic disorders :
Dysglycemia (
glycated hemoglobin & gt;=5.7 % ) , HyperNonHDLc ( non - high - density - lipoprotein [
HDL ]
cholesterol & gt;=4.14 mmol / L , or taking anticholesteremic medications ) ,
Hypertension (
systolic blood pressure & gt;=140 mm Hg or diastolic blood pressure & gt;=90 mm Hg , or taking antihypertensive medications ) , HyperALT (
alanine transaminase & gt;=
p75 [ 75th percentile , sex - specific ] ) , and
HyperGGT (
gamma - glutamyltransferase & gt;=
p75 [ sex - specific ] ) . -
pubmed
Selenium Augments microRNA Directed Reprogramming of Fibroblasts to Cardiomyocytes via
Nanog . -
pubmed
Removal of
insulin -
transferrin -
selenium completely inhibited the effect of reprogramming media upon cardiac gene expression and the addition of
selenium to standard culture media recapitulated the effects of RM . -
pubmed
Transient
lymphopenia is one hallmark of
sepsis , and emergent data indicate the
CD4 T cell compartment in
sepsis survivors is numerically and functionally altered ( when examined at the
Ag - specific level ) compared with nonseptic control subjects . -
pubmed
Previous data from our laboratory demonstrated
Ag - independent ,
lymphopenia - induced homeostatic proliferation to be a contributing mechanism by which
CD4 T cells numerically recover in
sepsis survivors . -
pubmed
However , we reasoned it is also formally possible that some
CD4 T cells respond directly to
Ag expressed by gut - resident microbes released during polymicrobial
sepsis . -
pubmed
Thus , our data demonstrate that the numerical recovery and functional responsiveness of
Ag - specific
CD4 T cells in
sepsis survivors is , in part , modulated by the intestinal barrier 's health discreetly defined by individual bacterial populations of the host 's microbiome . -
pubmed
Charged
Arg61 /
Arg196 and
Glu80 /
Glu215 on the conserved motif of
Tl - gal N - terminal
CRD and C - terminal
CRD are critical
amino acids for recognizing
carbohydrate binding , and the residues can affect protein folding and structure . -
pubmed
RESULTS : Our analysis revealed the lack of clinically relevant interactions between preparations of
levodopa and
benserazide ( used for the treatment of
PD ) and
angiotensin - converting enzyme inhibitors , antagonists of
AT1 receptor for
angiotensin II or antagonists of
beta - adrenoreceptors ( beta - adrenolytics ) . -
pubmed
In contrast , knockdown of
PPARD using small interfering RNA dramatically decreased the expression of genes related to
fatty acid activation (
ACSL1 ) and lipid formation (
PLIN2 ) and increased the expression of genes related to
fatty acid transport (
FABP3 ) and
triacylglycerol synthesis (
AGPAT6 and
DGAT1 ) . -
pubmed
The expression of genes related to
fatty acid synthesis (
FASN ) , hydrolysis (
PNPLA2 ) , and
fatty acid oxidation (
CPT1A ) was downregulated significantly only after knockdown of
PPARD in cells incubated with GW . -
pubmed
Group I (
paracetamol group ) received 15 mg / kg/6 h
IV paracetamol infusion for 3 days . -
pubmed
Group III (
indomethacin group ) received 0.2 mg / kg/12 h
indomethacin IV infusion for three doses . -
pubmed
Competition binding studies were performed using cell lysates with high
SV2A expression and
[ 3 H ] brivaracetam as a radioligand . -
pubmed
Subsequent concentration - inhibition curves revealed the antihistaminic prodrug
loratadine ( Ki = 1.16 muM ) and the antimalarial drug
quinine ( Ki = 2.03 muM ) to be the most potent
SV2A protein ligands of the investigated drug library . -
pubmed
A pharmacophore model was established , which indicated steric and electronic conformities of
brivaracetam with the new
SV2A ligands , and preliminary structure - activity relationships were determined . -
pubmed
The anti -
convulsive effects of the natural product
quinine may - at least in part - be explained by interaction with
SV2A. -
pubmed
The estimated 5 - year major amputation rate was lower in the
DES arm ( 19.3 % versus 34.0 % for
PTA - BMS ; P=0.091 ) . -
pubmed
The 5 - year rates of amputation- and event - free survival ( survival free from major amputation or reintervention ) were significantly higher in the
DES arm compared with
PTA - BMS ( 31.8 % versus 20.4 % , P=0.043 ; and 26.2 % versus 15.3 % , P=0.041 , respectively ) . -
pubmed
The aim of this study was to develop a controlled release drug - in - adhesive patch containing
escitalopram (
ESP ) using ion - pair technique . -
pubmed
The controlled release of
ESP was attributed to the interactions among
ESP - counter ion - PSA by
hydrogen bonding , and counter ion enhanced the interaction between
ESP and
PSA molecule , which acted as a " bridge " between them . -
pubmed
Using PERCIST criteria , 14 lesions ( 78 % ) showed a metabolic response in
PSMA PET with a reduction of SUV of at least 30 % , as well as a significant decrease in lesion size ; in seven of these lesions , no uptake of
68Ga - PSMA was detectable . -
pubmed
The expression of
HbMT2b , an isoform present in latex , decreased significantly in the latex following the stress - inducing treatments of tapping , wounding and
ethephon ( an
ethylene generator ) . -
pubmed
Inhibitors of the
sodium - glucose co - transporter-2 (
SGLT-2 ) are a novel class of
glucose - lowering agents that show promising results . -
pubmed
Among all blood
glucose lowering drugs , the proportional reporting ratio (
PRR ) was increased only for
SGLT-2 inhibitors ( 5.55 [ 4.23 , 7.29 ] ) . -
pubmed
While we observed an expected signal for
canagliflozin ( 7.09 [ 5.25 , 9.57 ] ) , the
PRR was also high for
empagliflozin ( 4.96 [ 2.89 , 8.50 ] ) and , for toe amputations only , for
dapagliflozin ( 2.62 [ 1.33 , 5.14 ] ) . -
pubmed
Carbon dioxide conversion mediated by
transition metal complexes continues to attract much attention because of its future potential utilization as a nontoxic and inexpensive
C1 source for the chemical industry . -
pubmed
Our research is inspired by the efficient enzymatic
CO2 catalysis occurring at the active site of
carbon monoxide dehydrogenase . -
pubmed
YAP Tyrosine Phosphorylation and Nuclear Localization in
Cholangiocarcinoma Cells Are Regulated by
LCK and Independent of
LATS Activity . -
pubmed
YAP is known to be regulated by a
serine /
threonine kinase relay module (
MST1 / 2 -
LATS1 / 2 ) culminating in phosphorylation of
YAP at
Serine 127 and cytoplasmic sequestration . -
pubmed
However ,
YAP also undergoes
tyrosine phosphorylation , and the role of
tyrosine phosphorylation in
YAP regulation remains unclear . -
pubmed
YAP was phosphorylated on
tyrosine 357 ( Y357 ) in CCA cell lines and
PDX models . -
pubmed
SRC family kinase (
SFK ) inhibition with
dasatinib resulted in loss of
YAPY357 phosphorylation , promoted its translocation from the nucleus to the cytoplasm , and reduced
YAP target gene expression , including cell lines expressing a
LATS1/2 - resistant
YAP mutant in which all
serine residues were mutated to
alanine . -
pubmed
Finally ,
dasatinib displayed therapeutic efficacy in
PDX models . -
pubmed
Members of the
Copper Metabolism Murr1 Domain containing ( COMMD ) family of 10 proteins are known to interact with
ENaC. -
pubmed
Specifically , we review the role of endovascular therapy in the prevention of
post - thrombotic syndrome following acute lower limb
deep vein thrombosis (
DVT ) ( ATTRACT trial ) , the efficacy of
edoxaban as the first direct oral anticoagulant used for the treatment of
cancer - associated
thrombosis ( HOKUSAI
VTE -
Cancer study ) , whether
aspirin can be considered for thromboprophylaxis
post - major orthopedic surgery ( EPCAT-2 trial ) , and the need for anticoagulant therapy for treatment of isolated distal
DVT ( CACTUS trial ) . -
pubmed
Citric Acid Capped
CdS Quantum Dots for Fluorescence Detection of
Copper Ions (
II ) in Aqueous Solution . -
pubmed
This transfer resulted in a blue shift of the excitonic photoluminescence (
PL ) peak generated by
MoS2 monolayers , together with an intensity increase . -
pubmed
This suspension technique also allowed the observation of
PL resulting from interlayer excitons in the
MoS2 /
WS2 vdW heterostructures . -
pubmed
SA /
CdS-2 ( mass ratio
SA :
CdS = 1:2 ) exhibited the highest reduction efficiency of
Cr ( VI ) , which could reduce the concentration of
Cr ( VI ) to 0 mg / L after 60 min illumination . -
pubmed
Morphine , which acts through
opioid receptors , is one of the most efficient analgesics for the alleviation of severe
pain . -
pubmed
On the bases of the in vitro biological activities of the hybrids , two analogs , opioid agonist /
NK1 antagonist
Tyr -[ d - Lys - Phe - Phe - Asp ]- Asn - d - Trp - Phe - d - Trp - Leu - Nle - NH2 ( 2 ) and opioid agonist /
NK1 agonist
Tyr -[ d - Lys - Phe - Phe - Asp ]- Gln - Phe - Phe - Gly - Leu - Met - NH2 ( 4 ) , were selected for in vivo tests . -
pubmed
When the analysis was restricted to those who sustained remission , exposure to
olanzapine compared with placebo was associated with significant decreases in cortical thickness in the left hemisphere ( beta [
SE ] , 0.04 [ 0.009 ] ; t34.4 = 4.7 ; P & lt;.001 ) , and the right hemisphere ( beta [
SE ] , 0.03 [ 0.009 ] ; t35.1 = 3.6 ; P & lt;.001 ) . -
pubmed
Switchable presentation of
cytokines on electroactive
polypyrrole surfaces for hematopoietic stem and progenitor cells . -
pubmed
By a stepwise Fourier transform infrared spectroscopy , the reaction between
MXene and
NBR was proved . -
pubmed
At only 2.8 vol%
MXene , a swelling ratio of 1.61 was achieved , representing a 75 % reduction compared to
NBR / reduced
graphene oxide and
carbon nanotube nanocomposites containing the same filler fraction . -
pubmed
Overall , emulsion compounding proved to be an efficient technique for forming
NBR /
MXene nanocomposites . -
pubmed
The revealed reaction between
MXene and
NBR to create functional polymer nanocomposites would provide a platform for utilising
MXene in other polymers . -
pubmed
Combination antiviral therapy with
lopinavir /
ritonavir ,
arbidol and
interferon - alpha1b for COVID-19 . -
pubmed
A dual - combination of
ribavirin and
interferon - alpha has been the widely used regimen for the treatment of this disease in China . -
pubmed
Herein , we report a semiconductive , proton - conductive , microporous
hydrogen - bonded organic framework (
HOF ) derived from
phenylphosphonic acid and
5,10,15,20 - tetrakis [ p - phenylphosphonic acid ] porphyrin (
GTUB5 ) . -
pubmed
This study aimed to determine the relation of best corrected visual acuity ( BCVA ) and low luminance visual acuity ( LLVA ) in
proliferative diabetic retinopathy (
PDR ) following treatment with either
aflibercept or pan - retinal photocoagulation (
PRP ) . -
pubmed
Post aflibercept therapy , the mean change in BCVA and LLVA at 52 weeks after
aflibercept was +2.1 ( SD 6.05 ) letters and +0.39 ( SD 5.6 ) letters , respectively . -
pubmed
The principal metabolite was
( R )-(-)- 2 - phenylhydantoic acid , formed stereo - specifically in a ring - opening reaction of
( R )- 5 - phyenylhydantoin by
dihydropyrimidinase (
EC 3.5.2.2 ) . -
pubmed
Exogenous
cyclic AMP as well as positive effectors of the
adenylyl cyclase system were able to overcome the
phosphate suppression . -
pubmed
The change in
enzyme activity as a result of the
enzyme -
haptene -
antibody reaction is proportional to the concentration of the
haptene . -
pubmed
In this modified method ,
cortisol was released from its
binding globulin by enzymatic degradation rather than by extraction with
ether , and a preincubated mixture of first and second antibody was used to separate
antibody - bound
cortisol from free . -
pubmed
Despite the recognition of a
traS + (
Slo+ ) component of DNA within that encoding the
Slo+ and
Sfx+ phenotypes , this gene seems nevertheless to be responsible for the
Sfx+ phenotype since
hydroxylamine - induced
Slo- mutants of pVS438 are usually also
Sfx- . -
pubmed
Therefore , we quantitated the
tryptophan metabolites ,
L - kynurenine and
quinolinic acid , and we measured
neopterin , a marker of
interferon - gamma (
IFN - gamma ) , in blood obtained during the acute phase of each
syndrome . -
pubmed
Our data suggest that
indoleamine-2,3 - dioxygenase (
IDO ) , the rate limiting enzyme of the
kynurenine pathway of
L - tryptophan metabolism , was activated in both
syndromes by
cytokines including
IFN - gamma , and that perhaps products of
tryptophan metabolism played a role in the pathogenesis of
EMS and
TOS . -
pubmed
Some alternative approaches , including meta - regression techniques , are discussed , and illustrated with reference to examples of meta - analyses of studies of
breast cancer risk following
oral contraceptive use and of patterns of
post - bereavement mortality . -
pubmed
Protein kinase A in the absence of
ATP had no effect on channel activity ( n = 3 ) . -
pubmed
We tested the effect of two potent inhibitors of
protein phosphatases ,
calyculin A and
okadaic acid . -
pubmed
Calyculin A appeared to be less effective in increasing open probability than
okadaic acid , suggesting that the
phosphatase involved is neither type 1 , 2A , nor 2B. -
pubmed
Calyculin A in the absence of
protein kinase A was ineffective . -
pubmed
Study I : The impact of
metoprolol CR /
ZOK 50 mg once daily for 4 weeks compared with placebo 24 hours
post dosing was to produce a significant reduction in supine ( P = .0001 ) , and standing ( P less than .0001 )
systolic blood pressures and the standing
diastolic pressure ( P = .035 ) . -
pubmed
The supine
diastolic pressure was lower after
metoprolol CR /
ZOK but not significantly so . -
pubmed
Study
II :
Metoprolol CR /
ZOK 50 mg daily given to elderly
hypertensives when compared with placebo 24 hours
post dosing produced a significant fall in supine ( P = .022 ) and standing ( P = .022 )
diastolic pressure . -
pubmed
Fixed schistosomula coated with FPLC - purified
IgE and
IgG gave 7.6 + /- 0.4 and 6.0 + /- 0.1 pmoles of
LTC4 per 10(6 ) low - density eosinophils , respectively . -
pubmed
Following preincubation with
platelet - activating factor (
PAF ) ( 10(-7 ) M ) and
leukotriene B4 (
LTB4 ) ( 10(-7 ) M ) , normal density eosinophils released
LTC4 when in contact with larvae coated with
antigen - specific IgE. -
pubmed
The synthetic chemotactic tripeptide
formyl - methionyl - leucyl - phenylalanine (
FMLP ) had no influence on
IgE - dependent release of
LTC4 from eosinophils . -
pubmed
In contrast ,
FMLP ( 10(-7 ) M ) enhanced the
IgG - dependent LTC4 release , with
PAF and
LTB4 also showing a small enhancing effect . -
pubmed
Baseline values and
post -
DDAVP increase were not significantly different from our normal controls . -
pubmed
The specificity of the anti -
Au ( 1 ) serum used in this study was ascertained by immunodiffusion and immunoelectrophoresis , and by immunoelectron microscopic studies of the antigen - antibody complex prepared in vitro by mixing the
ferritin - conjugated anti -
Au ( 1 ) reagent with the purified
Au ( 1 ) particles . -
pubmed
Kinetic studies and characteristics of a modifier of
Ca2 + -stimulated
ATPase . -
pubmed
The thermostable modifier increases the
Mg2 + -stimulated
ATPase with a parallel decrease of
Ca2 + -stimulated
ATPase . -
pubmed
The modifier inhibited
Ca2 + -stimulated
ATPase by diminishing the velocity without any significant effect on the apparent affinity of binding of
Ca2 + . -
pubmed
The inhibition of
Ca2 + -stimulated
ATPase was independent of
Ca2 + concentration and could not be reversed by increasing
Ca2 + levels . -
pubmed
Monovalent cations and
calmodulin increased the
Mg2 + -stimulated
ATPase in the presence of the modifier . -
pubmed
Thus ,
H-2 antigens are not only recognized by allogenic lymphocytes leading to generation of cytotoxic T lymphocytes ( CTLs ) , but it has also been demonstrated that in syngeneic systems most T cells are only able to recognize foreign
antigens in conjunction with their own
MHC (
major histocompatibility complex ) antigens . -
pubmed
The deduced
amino acid sequence of
TUB2 had a high degree of homology to other fungal beta - tubulins . -
pubmed
The urinary excretion of endogenous
creatinine ( CRTN ) and
3 - methylhistidine ( 3 - MH ) has been proposed as a predictor of fat - free mass (
FFM ) in healthy subjects . -
pubmed
CRTN excretion was found well associated with
FFM (
R2 = 0.89 ; P & lt ; 0.0001 ) , whereas
3 - MH was lightly associated with
FFM (
R2 = 0.33 ; P & lt ; 0.01 ) . -
pubmed
Residuals from the regression of 3 - MH vs.
FFM were found to be correlated with CRTN excretion and
FFM (
R2 = 0.57 and 0.67 , respectively ) , suggesting that muscularity and the absolute amount of lean mass are relevant for the error in predicting
FFM from
3 - MH excretion . -
pubmed
Data also suggest that the relationship between
3 - MH and
FFM is complex , limiting the possibility of predicting body composition from the excretion of this metabolite . -
pubmed
Down - modulation of
interleukin-6 gene expression by
17 beta - estradiol in the absence of high affinity DNA binding by the
estrogen receptor . -
pubmed
The mechanism of repression of the interleukin-6 ( IL-6 ) promoter by
17 beta - estradiol (
E2 ) was investigated in cells transfected with wild - type ( wt ) or mutant
estrogen receptor (
ER ) expression vectors . -
pubmed
This was in contrast to the absolute dependence of
ER on its own DNA binding domain for activation of typical
estrogen response element - containing promoters , as reported previously by other investigators . -
pubmed
Furthermore , the repression of the IL-6 promoter by a combination of
ER and
E2 , unlike activation of
estrogen response elements by the same combination , did not appear to be mediated via high affinity binding of the receptor to the promoter . -
pubmed
Observations on the
HDL system in these two families substantiate the role of
cholesteryl ester /
triglyceride exchange between
HDL and
lower - density lipoproteins in the remodelling of
HDL in plasma . -
pubmed
Analysis of variance using a linear model demonstrated no significant intergroup differences in
O2- production by
lymphokine - activated monocytes from G2 pigs . -
pubmed
Litter contributed significantly to variation in monocyte
O2- production in G2 pigs ( P & lt ; or = 0.005 ) and
SLA - DQ ( P & lt ; or = 0.01 ) expression . -
pubmed
Cyclic sulfolanes as novel and high affinity
P2 ligands for
HIV-1 protease inhibitors . -
pubmed
The level of
LSP1 RNA in
tumors developing & lt;110 days after injection of
MNU was 19.1 % + /- 5.2 ( mean + /- SEM ) , while the level of
LSP1 in later
tumors was 78.4 % + /- 13.0 ( P = 0.004 ) . -
pubmed
Third , they show that there are two classes of
T - lymphoma , which differ in their expression of
LSP1 RNA and that the down - regulation of
LSP1 is specifically associated with the early appearance of
T - lymphoma after injection with
MNU . -
pubmed
Of the specific inhibitors of
iNOS in infarcted heart muscle ,
S - methylisothiourea (
SMT ) was the most potent . -
pubmed
Dexamethasone and
cyclosporin A failed to inhibit
iNOS . -
pubmed
Two
H3 receptor agonists ,
R -(-)- alpha - methylhistamine and
imetit , inhibited the electrically induced
increase in diastolic blood pressure in a dose - dependent manner . -
pubmed
Two
H3 receptor antagonists ,
thioperamide 1 mumol / kg and
clobenpropit 0.1 mumol / kg , attenuated the inhibitory effect of
imetit . -
pubmed
The neurogenic vasopressor response was increased by about 15 % by
thioperamide 1 mumol / kg and
clobenpropit 0.1 mumol / kg and decreased by 25 % by the
histamine methyltransferase inhibitor
metoprine 37 mumol / kg . -
pubmed
Metoprine had only a very low affinity for
H3 binding sites ( labelled by
3H - N alpha - methylhistamine ; pKi 4.46 ) . -
pubmed
Moreover , it demonstrates that these
H3 receptors are probably activated by endogenous
histamine and appear to be operative in
hypertension . -
pubmed
In
pectate lyases , this cleft is rich in charged residues and contains an elongated ribbon of positive potential when
Ca2 + is bound . -
pubmed
Pectin lyase A does not bind
Ca2 + but an
arginine residue is found in an equivalent position to the
Ca2 + ion in
pectate lyase , suggesting a similar role in catalysis . -
pubmed
The
AsD2 radical was generated in a free - space cell by dc - glow discharge of a gaseous mixture of
D2 and
He over
arsenic powder . -
pubmed
The harmonic force field was used to calculate vibrational corrections to the rotational constants of both species and thus the zero - point average structures for both species were derived ; AsH2 : rz(AsH ) = 1.53436(15 ) A , thetaz(
HAsH ) = 90.695(20 ) degrees , and
AsD2 : rz(AsD ) = 1.52902(13 ) A , thetaz(DAsD ) = 90.722(17 ) degrees , where the errors in parentheses are due to residual inertial defects of the corrected moments of inertia . -
pubmed
From the difference between the rz structures for
AsH2 and AsD2 the re structure of
AsH2 is estimated to be re(
AsH ) = 1.5158(6 ) A , thetae(HAsH ) = 90.79(8 ) degrees . -
pubmed
administration of CA -
SLN and a
camptothecin control solution ( CA - Sol ) . -
pubmed
Neutrophil - induced changes in the biomechanical properties of endothelial cells : roles of
ICAM-1 and reactive
oxygen species . -
pubmed
This study evaluated the changes in the biomechanical properties of endothelial cells ( ECs ) induced by neutrophil adhesion and the roles of
ICAM-1 and reactive
oxygen species ( ROS ) in modulating these changes . -
pubmed
The neutrophil - induced increase in the apparent stiffness of ECs was inhibited with 1 %
DMSO ( a
hydroxyl radical scavenger ) ,
allopurinol ( a
xanthine oxidase inhibitor ) , or
deferoxamine ( an
iron chelator ) , suggesting that ROS may be involved in mediating the EC stiffening response . -
pubmed
This ROS production in ECs was completely prevented by the
anti - ICAM-1 Ab and partially inhibited by
allopurinol . -
pubmed
Full nucleotide and
amino acid sequences were considered as well as abridged sequences from the hypervariable peptide binding region , known to interact in vivo , with
HLA presented foreign peptide . -
pubmed
HLA - A alleles ( n = 60 ) assorted into 12 groups based on full nucleotide and
amino acid sequence which with few exceptions recapitulated serological groupings , however the patterns were largely discordant with clusters prescribed by
PBR sequences . -
pubmed
Expression of
CD16b , a low affinity
Fcgamma receptor , was established by either transfection or spontaneous insertion via its
glycosylphosphatidylinositol anchor . -
pubmed
Calcium - sensitive potassium channel inhibitors antagonize
genistein- and
daidzein - induced arterial relaxation in vitro . -
pubmed
The method , based on the precipitation of
amine - oxidase - generated
H2O2 by
CeCl3 , was shown to be specific for
DAO and PAO and permitted their localization in plant tissues with a high degree of resolution . -
pubmed
The
oxidases could provide
H2O2 for
peroxidase - mediated cross - linking reactions in the cell wall and may , in this capacity , play a role in the regulation of plant growth . -
pubmed
The kinetics of
human polymerase beta (
pol beta ) binding to the single - stranded DNA , in the ( pol beta)(16 ) and ( pol beta)(5 ) binding modes , that differ in the number of occluded
nucleotide residues in the protein - DNA complexes , have been examined , using the fluorescence stopped - flow technique . -
pubmed
Their morning fasting urine were collected for measurement of
iodide concentration , and their sera were measured for
thyroid - stimulating hormone(
TSH , the third generation ) ,
thyroid peroxidase antibody (
TPOAb ) ,
thyroglobulin antibody (
TGAb ) and
thyroglobulin (
TG ) . -
pubmed
RESULTS : The median level of urinary
iodide of the sample subjects was 614.61 micrograms / L. The prevalence of clinical and subclinical
hyperthyroidism was 1.21 % and 1.12 % respectively ; 92.3 % of the clinical
hyperthyroidism were of
Graves ' disease and 75 % of the subjects with subclinical
hypothyroidism were
TRAb positive . -
pubmed
Fracture characteristics of
carbon fibre , ceramic and non -
palladium endodontic
post systems at monotonously increasing loads . -
pubmed
A
carbon fibre
post system , three non -
palladium and one
palladium metal
post systems , two ceramic
post systems , and a metal
post system with a ceramic core were studied in vitro . -
pubmed
The highest mean
fracture loads were found for the
carbon fibre
post system ( 312.5 + /- 58.8 N ) . -
pubmed
The
fracture load of non -
palladium metal
posts ( 242.3 - 300.4 N ) did not differ significantly from that of the Perma - dor
post ( 265.9 N ) , which does contain
palladium . -
pubmed
Values of 300.3 + /- 89.3 N (
aluminium oxide ceramics ) and 193.5 + /-57.0 N (
zirconia ceramics ) were found for the ceramic
posts . -
pubmed
The genomic DNA was amplified by
polymerase chain reaction ( PCR ) and the products were separated on 1.5 %
agarose gel and visualized by ultraviolet transillumination . -
pubmed
Furthermore , lowering
adenosine levels or antagonizing
adenosine receptors can reverse pulmonary phenotypes in this model , suggesting that chronic
adenosine elevations can affect signaling pathways that mediate aspects of
chronic lung disease . -
pubmed
Carbodiimide may form intrahelical and interhelical crosslinks within or between tropocollagen molecules , whereas
genipin may further introduce intermicrofibrillar crosslinks between adjacent
collagen microfibrils . -
pubmed
The present study has shown that this inhibitory profile , referred to as
bacteriocin producer ( P )- type 777 activity , is due to the type
A1 lantibiotic
streptin . -
pubmed
The fully processed form of
streptin (
streptin 1 ) is a
23 - amino - acid peptide with a mass of 2,424 Da . -
pubmed
However , several
PAHs enhanced
histamine release and
IL-4 production in response to crosslinking the
high - affinity IgE receptor ,
Fc epsilon RI . -
pubmed
The enhancement seen with
1,6 - BaP - quinone involved an increase in
tyrosine phosphorylation in several different substrates , including the
Fc epsilon RI - associated tyrosine kinase ,
Lyn , and elevated reactive
oxygen species ( ROS ) levels detected by
dichlorofluorescein fluorescence and flow cytometry . -
pubmed
Effects of different acid and
sodium hypochlorite treatments on dentin
collagen : a FEISEM analysis . -
pubmed
Plasma
RLP -
cholesterol was measured by an immunoabsorption enzyme method . -
pubmed
The analytical procedures employed would also have detected
ethylene glycol dinitrate (
EGDN ) if present at levels greater than 2 ng ,
nitrobenzene (
NB ) if present at levels greater than 50 ng ,
mononitrotoluenes if present at levels greater than 50 ng , and the other common isomers of
dinitrotoluene if these had been present at levels in excess of 10 ng . -
pubmed
The relatively high volatility of
EGDN ,
NB , and the
mononitrotoluenes would , however , cause traces of these compounds to disperse rapidly . -
pubmed
The
cystic fibrosis ( CF ) transmembrane conductance regulator (
CFTR ) plays a crucial role in mediating duodenal
bicarbonate (
HCO ( 3 )(- ) ) secretion (
DBS ) . -
pubmed
To explain this apparent paradox , we hypothesized that
CFTR dysfunction increases cellular [
HCO ( 3 )(- ) ] and buffering power . -
pubmed
DBS was measured in situ using a standard loop perfusion model with a pH stat under
isoflurane anesthesia . -
pubmed
Acid - induced
DBS was abolished at 1 and 3 h and was reduced 24 h after treatment with
CFTR(inh)-172 , although basal
DBS was increased at 24 h.
CFTR(inh)-172 treatment had no effect on gastric acid or
HCO ( 3 )(- ) secretion . -
pubmed
Several studies have demonstrated the existence of an autonomous intranuclear
phospho -
inositide cycle that involves the activation of
nuclear PI - PLC and the generation of
diacylglycerol (
DG ) within the nucleus . -
pubmed
Rates of
hypertension , high
low density lipoprotein (
LDL )
cholesterol , and
diabetes mellitus awareness , treatment , and control for persons with a history of
myocardial infarction and
stroke were compared by using two nationally representative samples of the US population : the Third National Health and Nutrition Examination Survey in 1988 - 1994 ( n = 1,004 ) and the National Health and Nutrition Examination Survey in 1999 - 2002 ( n = 512 ) . -
pubmed
Among such survivors , awareness of a previous diagnosis of
hypertension and prevalence of self - reported
diabetes mellitus remained stable while awareness of high
LDL cholesterol increased from 43.3 % to 60.2 % ( p & lt ; 0.01 ) . -
pubmed
Among those aware of their diagnosis , pharmacologic treatment for high
LDL cholesterol increased from 33.1 % to 78.4 % and pharmacologic treatment for
diabetes mellitus increased from 80.0 % to 93.6 % during this time ( each p & lt ; 0.01 ) , while pharmacologic treatment for
hypertension increased nonsignificantly . -
pubmed
Among those receiving pharmacologic treatment ,
hypertension and high
LDL cholesterol control increased from 48.9 % to 59.3 % ( p = 0.05 ) and from 5.1 % to 33.1 % ( p & lt ; 0.01 ) , respectively . -
pubmed
The number of US
myocardial infarction and
stroke survivors increased between 1988 - 1994 and 1999 - 2002 , and substantial improvements occurred in the awareness , treatment , and control of high
LDL cholesterol in this population . -
pubmed
This article examines the rationale for the use of the oral dual
endothelin receptor antagonist
bosentan in this life - threatening condition . -
pubmed
Interaction of
riluzole with the closed inactivated state of
Kv4.3 channels . -
pubmed
The effect of
riluzole on
Kv4.3 was examined using the whole - cell patch -
clamp technique . -
pubmed
Riluzole inhibited the peak amplitude of
Kv4.3 in a reversible , concentration - dependent manner with an IC(50 ) of 115.6 microM. -
pubmed
tau ( f ) was not altered by
riluzole at concentrations up to 100 microM , but tau(s ) became slower with increasing
riluzole concentration , resulting in the crossover of the currents . -
pubmed
Riluzole shifted the voltage dependence of the steady - state inactivation of
Kv4.3 in the hyperpolarizing direction in a concentration - dependent manner . -
pubmed
The K(i ) for
riluzole for interacting with the inactivated state of
Kv4.3 was estimated from the concentration - dependent shift in the steady - state inactivation curve and was determined to be 1.2 muM. -
pubmed
Riluzole induced a significant use - dependent inhibition of
Kv4.3 . -
pubmed
These results suggest that
riluzole inhibits
Kv4.3 by binding to the closed inactivated state of the channels and that the unbinding of
riluzole occurs from the closed state during depolarization . -
pubmed
Mitochondrial complex II (
succinate dehydrogenase [
SDH ] ) is part of the
tricarboxylic acid cycle and the respiratory electron transport chain . -
pubmed
A functionally conserved basic residue in
glutathione transferases interacts with the
glycine moiety of
glutathione and is pivotal for enzyme catalysis . -
pubmed
The present study characterized conserved residues in a
GST (
glutathione transferase ) in the active - site region that interacts with
glutathione . -
pubmed
In the
GSTD ( Delta class
GST ) studied ,
adGSTD4 - 4 , the network consisted of
His(38 ) ,
Met(39 ) ,
Asn(47 ) ,
Gln(49 ) ,
His(50 ) and
Cys(51 ) . -
pubmed
CONCLUSION : The targeted magnetic nanoparticles containing
5 - FU can improve the chemotherapeutic effect of
5 - FU against
hepatocellular carcinoma by decreasing the expression of bcl-2 gene , and increasing the expression of
bax and
caspase 3 genes . -
pubmed
We prospectively examined the relationship between
fructose intake and incident
kidney stones in the Nurses ' Health Study I (
NHS I ) ( 93,730 older women ) , the Nurses ' Health Study
II (
NHS II ) ( 101,824 younger women ) , and the Health Professionals Follow - up Study ( 45,984 men ) . -
pubmed
Sequence analysis showed a single nucleotide deletion in the
89.6 env gene of the mutant that caused a frame shift and truncation of 115
amino acids from the cytoplasmic domain . -
pubmed
Although a shift from
fatty acids (
FAs ) to
carbohydrates ( CHOs ) is considered beneficial for the diseased heart , it is unclear why subjects with
FA beta - oxidation defects are prone to
cardiac decompensation under stress conditions . -
pubmed
Carbon-13 methodology was used to assess substrate flux through energy - yielding pathways in hearts perfused ex vivo at two workloads with a physiological substrate mixture mimicking the fed state , and real - time RT - quantitative
polymerase chain reaction was used to document the expression of selected metabolic genes . -
pubmed
At the metabolic level , beyond the expected shift from
FA ( 5 - fold down ) to
CHO ( 1.5 - fold up ; P & lt ; 0.001 ) at both preloads ,
PPARalpha null hearts also displayed 1 ) a significantly greater contribution of exogenous
lactate and
glucose and/or
glycogen ( 2 - fold up ) to endogenous
pyruvate formation , whereas that of exogenous
pyruvate remained unchanged and 2 ) marginal alterations in
citric acid cycle - related parameters . -
pubmed
Collectively , our data highlight the importance of metabolic alterations in
CHO metabolism associated with
FA oxidation defects as a factor that may predispose the heart to decompensation under stress conditions even in the fed state . -
pubmed
Label - free
calcium imaging in ischemic retinal tissue by
TOF - SIMS . -
pubmed
Thus , the
TOF - SIMS technique provides a tool for the study of intercellular communication by
Ca ( 2 + ) ion movement . -
pubmed
A combined chemotherapy regimen comprising
fludarabine ,
cytosine arabinoside , and
granulocyte colony - stimulating factor (
FLAG ) has been used in the treatment of relapsed or
refractory leukemias . -
pubmed
Germination of the two genotypes was differentially sensitive to
abscisic acid (
ABA ) and
gibberellin (
GA ) at elevated temperatures . -
pubmed
Expression of many genes involved in
ABA ,
GA , and
ethylene biosynthesis , metabolism , and response was differentially affected by high temperature and light in the two genotypes . -
pubmed
ATH1 , and
NTH1 hydrolyze
trehalose to
glucose to provide energy and assist in recovery from stress . -
pubmed
ATH1Delta and
NTH2Delta showed very low neutral
trehalase activity and
NTH1Delta did not show any neutral
trehalase activity , and
trehalose concentrations were higher . -
pubmed
Increased neutral
trehalase activity ( equivalent to the wild type ) , reduction of
trehalose content and brisk sensitivity to stressors were noted in
TREH -
ATH1Delta strain , but not in
TREH -
NTH1Delta or -
NTH2Delta . -
pubmed
Our results suggest that
TREH acts as a stress - response protein in the kidney rather than involved in utilization of exogenous
trehalose . -
pubmed
BACKGROUND INFORMATION : The
TSPO ( 18 kDa
translocator protein ) is a mitochondrial transmembrane protein involved in
cholesterol transport in organs that synthesize
steroids and
bile salts . -
pubmed
Different natural and synthetic high - affinity
TSPO ligands have been characterized through their ability to stimulate
cholesterol transport , but also to stimulate other physiological functions including cell proliferation , apoptosis and
calcium - dependent transepithelial ion secretion . -
pubmed
Enterocyte cytoplasm also contained the endogenous
TSPO ligand , polypeptide
DBI (
diazepam - binding inhibitor ) . -
pubmed
Whereas intestinal
TSPO had high affinity for the synthetic ligand
PK 11195 , the pharmacological profile of
TSPO in the duodenum was distinct from the jejunum and ileum . -
pubmed
Specifically ,
benzodiazepine Ro5 - 4864 and
protoporphyrin IX showed 5 - 13 - fold lower affinity for duodenal
TSPO . -
pubmed
CONCLUSIONS : The functional differences in subpopulations of
TSPO in different regions of the intestine could be related to structural organization of mitochondrial protein complexes that mediate the ability of
TSPO to modulate either
chloride secretion or absorption in the duodenum and ileum respectively . -
pubmed
MYB134 - overexpressing plants exhibited a concomitant reduction in
phenolic glycoside concentrations and other minor alterations to levels of small
phenylpropanoid metabolites . -
pubmed
Our investigation of
Ca ( 2 +)-signaling pathways involved in axon formation using cultured hippocampal neurons demonstrates a role for
Ca ( 2 +)/ calmodulin kinase kinase (
CaMKK ) and its downstream target
Ca ( 2 +)/ calmodulin kinase I (
CaMKI ) . -
pubmed
These results are consistent with a model in which highly localized
calcium influx through the
TRPC5 channels activates
CaMKK and
CaMKIgamma , which subsequently promote axon formation . -
pubmed
The induction of
FLT3 in
CML cells attenuated
imatinib - induced apoptosis . -
pubmed
These findings indicate that
FLT3 is associated with disease progression , despite
imatinib therapy . -
pubmed
A prominent defense against the oxidant burden caused by
CS is the
glutathione (
GSH ) adaptive response in the lung epithelial lining fluid ( ELF ) and tissue . -
pubmed
OBJECTIVES : Examine the effect of aging on the
GSH adaptive response to
CS and resulting lung sensitization to
inflammation and oxidation . -
pubmed
Study on nucleic acid (
CT - DNA and yeast
tRNA ) binding behaviors and cytotoxic properties of a heterodinuclear
Ru ( II )- Co ( III ) polypyridyl complex . -
pubmed
PURPOSE : To investigate the effect of Colloidal
Platinum Nanoparticles (
CPN ) on the bond strength between dentin and
4 - META /
MMA - TBB resin using different concentrations of
CPN . -
pubmed
One control and four experimental groups ( 2 specimens per group ) were used as follows : a ) dentin surfaces treated with 10 - 3 solution , followed by rinsing with water and subsequently an acrylic rod bonded with hand - mixed 4META /
MMA - TBB resin ( Super - Bond C&B , Sun Medical ) ( control ) ; b ) dentin surfaces treated with 10 - 3 etching solution , followed by rinsing with water and application of
CPN ( 100 % or 10 % ) as a primer solution for 60 s and rinsed with water for 20 s , then an acrylic rod bonded with Super - Bond C&B(Etch -
CPN [ 100 % or 10 % ] ) ; c ) dentin surfaces treated with
CPN ( 100 % or 10 % ) for 60 s , rinsed with water for 20 s , followed by application of 10 - 3 solution , then an
acrylic rod bonded with Super - Bond C&B (
CPN - Etch [ 100 % or 10 % ] ) . -
pubmed
RESULTS : Etch -
CPN ( 100 ) ,
CPN - Etch(100 ) and
CPN - Etch ( 10 ) showed significantly higher bond strengths compared to the control . -
pubmed
The bond strength of
4META /
MMA - TBB resin was approximately doubled by
CPN application . -
pubmed
As compared with the model group , the abnormal changes of
TIMP-1 and
MMP1 expressions were ameliorated in the two groups treated either by BHR or
fosinopril ( P & lt ; 0.05 ) . -
pubmed
Translational characterization of
[ 11C ] GSK931145 , a PET ligand for the
glycine transporter type 1 . -
pubmed
Although all gels became degraded in the presence of
lysozyme , their degradation rate greatly depended on the
genipin load . -
pubmed
After extraction and separation of
hemoglobin from whole blood , the
polyacrylamide gel electrophoresis (
SDS - PAGE ) , cellulose acetate electrophoresis , and isoelectric focusing ( IEF ) were used to confirm
Hb variabilities in these fishes . -
pubmed
UV - vis spectrophotometery was also utilized to measure
oxygen affinity of
Hbs by
sodium dithionite . -
pubmed
Oxygen affinities of these
Hbs were compared using
Hb -
oxygen dissociation curves . -
pubmed
Together , these results demonstrate a significant relationship between
oxygen affinity of fish
hemoglobins and environmental partial pressure of
oxygen . -
pubmed
A case of wear and entrapment of a
polyethylene disc observed in a Starr - Edwards (
SE ) mitral caged - disc valve at 37 years after implantation is reported . -
pubmed
Cinefluoroscopy showed the
polyethylene disc of the
SE valve to have impinged against a calcified mass on the left ventricular posterior wall , causing a tipping motion of the disc during opening . -
pubmed
roseus WRKY transcription factor ,
CrWRKY1 , that is preferentially expressed in roots and induced by the phytohormones
jasmonate ,
gibberellic acid , and
ethylene . -
pubmed
Up - regulation of
TDC increased
TDC activity ,
tryptamine concentration , and resistance to
4 - methyl tryptophan inhibition of
CrWRKY1 hairy roots . -
pubmed
The
enzyme contains 1 mol
Zn ( 2 + ) in its active site and shows a unique
zinc dependence . -
pubmed
The fact that dehydration of
d - threonine and
d - allo - threonine , also catalyzed by
DsdSC , is likewise
Zn ( 2 + ) dependent indicates that
Zn ( 2 + ) is indispensable for the elimination of
hydroxyl group , regardless of the stereochemistry of C(beta ) . -
pubmed
Removal of
Zn ( 2 + ) results in a less polar active site without changing the gross conformation of
DsdSC . -
pubmed
Mutation of
Cys400 or
His398 within the
Zn ( 2 + ) binding sites to
Ala endowed
DsdSC with similar properties to those of the
Zn ( 2 + ) -depleted wild - type
enzyme : the mutants lost the reactivity toward
d - serine and
d - threonine but retained that toward
beta - Cl - d - alanine . -
pubmed
Our data suggest that
DsdSC catalyzes the alpha -
hydrogen abstraction and
hydroxyl group elimination in a concerted fashion . -
pubmed
LRH-1 plays important roles in embryonic development , steroidogenesis and
cholesterol homeostasis . -
pubmed
GREB1 is a well characterised
ERalpha target gene , with three
estrogen response elements ( ERE ) located on its promoter . -
pubmed
In the presence of
ICI 182,780 , an
ERalpha antagonist ,
LRH-1 still induced proliferation . -
pubmed
Utility of
FDG PET /
CT in the assessment of
myeloid sarcoma . -
pubmed
CONCLUSION : This article highlights the utility of
( 18 ) F - FDG PET /
CT , which has high sensitivity in detecting early
MS and provides a systemic overview of
tumor burden , and its potential role in monitoring of treatment response . -
pubmed
In this article , various approaches are explored towards a water compatible
MIP for the target molecule
l - nicotine . -
pubmed
In this study , we synthesized a novel synthetic
alpha - lipoic acid - decursinol (
ALA - DA ) hybrid compound , and compared neuroprotective effects of
ALA , DA or
ALA - DA against
ischemic damage in the gerbil hippocampal
CA1 region induced by 5 min of transient
cerebral ischemia . -
pubmed
However , 20 mg / kg
ALA - DA pre - treatment protected pyramidal neurons from
ischemic damage in the
CA1 region . -
pubmed
In addition , 20 mg / kg
ALA - DA pre - treatment markedly decreased the activation of astrocytes and microglia in the
CA1 region 4 days after
ischemic injury . -
pubmed
Deamidation of N - terminal
Gln by the
Ntaq1 Nt ( Q )- amidase is a part of the
Arg / N - end rule pathway , a
ubiquitin - dependent proteolytic system . -
pubmed
Here we identify Gln -
Usp1(Ct ) , the C - terminal fragment of the autocleaved
Usp1 deubiquitylase , as the first physiological
Arg / N - end rule substrate that is targeted for degradation through deamidation of N - terminal
Gln . -
pubmed
The autocleaved
Usp1 remains a deubiquitylase because its fragments remain associated with
Uaf1 , an enhancer of
Usp1 activity , until the Gln -
Usp1(Ct ) fragment is selectively destroyed by the
Arg / N - end rule pathway . -
pubmed
We also show that metabolic stabilization of
Gln -
Usp1(Ct ) results in a decreased monoubiquitylation of
PCNA and in a
hypersensitivity of cells to ultraviolet irradiation . -
pubmed
Thus , in addition to its other functions in DNA repair and chromosome segregation , the
Arg / N - end rule pathway regulates
genomic stability through the degradation - mediated control of the autocleaved
Usp1 deubiquitylase . -
pubmed
By substitution of three conserved
amino acids , found in all electrogenic isoforms , at corresponding sites in
NaPi - IIc , electrogenicity was re - established and the
Na(+)/P i stoichiometry increased from 2:1 to 3:1 . -
pubmed
Fluorometric recordings from
AAD - IIc mutants with
Cys substituted at functionally important sites established that
AAD - IIc undergoes substrate- and voltage - dependent conformational changes that correlated qualitatively with its presteady - state kinetics . -
pubmed
This study was designed to investigate the potential role of induction of
HSP70 using
geranylgeranylacetone (
GGA ) on
testicular damage caused by
CCl4 . -
pubmed
Serum
testosterone , testicular
lactate dehydrogenase (
LDH ) and
alkaline phosphatase (
ALP ) , testicular
malondialdehyde (
MDA ) , total
nitrite and total antioxidant capacity ( T - AOC ) were measured . -
pubmed
Group
II showed lower values of gonado - somatic index , serum
testosterone , testicular
LDH ,
ALP , T - AOC and greater values of testicular
MDA and total
nitrite than in control . -
pubmed
Group III had higher values of gonado - somatic index , serum
testosterone , testicular
LDH ,
ALP , T - AOC with less testicular
MDA and total
nitrite than in group
II . -
pubmed
These findings suggest that
GGA - induced activation of
HSP 70 significantly alleviate
CCl4 inflicting
testicular damage by
HSP 70 mediated cytoprotection and antioxidant effects . -
pubmed
Long - lasting distortion of
GABA signaling in
MS / DB neurons after binge - like
ethanol exposure during initial synaptogenesis . -
pubmed
The impact of
ethanol in vivo was long lasting as most changes in
MS / DB GABAAR mPSCs were still observed as late as PD 60 - 85 . -
pubmed
Maturing
MS / DB neurons in naive brain slices PD 4 - 16 showed increasing mPSC frequency , decay kinetics , and
zolpidem sensitivity that were nearly identical to our earlier findings in cultured septal neurons ( DuBois et al . -
pubmed
Previously , we showed
ethanol treatment of cultured embryonic day 20 septal neurons distorts the maturation of GABAAR mPSCs predicting that early stages of GABAergic transmission in
MS / DB neurons are vulnerable to
intoxication injury ( DuBois et al . -
pubmed
From eight
gp21 - analyzed sequences one
amino acid change ( Y477H ) was associated with the switch of a helix to
coil structure at secondary structure prediction . -
pubmed
From 10
HBZ analyzed sequences , two
amino acid changes were identified ( S9P and T95I ) at the activation domain . -
pubmed
It was found that the inclusion of
rIL-2 in the
R - CHOP regimen increased the effectiveness of the treatment of
B - cell NHL with a high - risk of poor disease 's course . -
pubmed
coli
strain containing
PAL1 ,
4CL1 , and CUS led to the production of
dicinnamoylmethane at a high level of 0.36 g / l. Supplement of
2 - fluoro - phenylalanine yielded two
fluorinated dicinnamoylmethane derivatives ,
6,6 '- difluoro - dicinnamoylmethane and
6 - fluoro - dicinnamoylmethane , of which the latter is a new
curcuminoid . -
pubmed
Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by
sodium dodecyl sulfate - polyacrylamide gel electrophoresis , and 28,800 Da determined by gel filtration . -
pubmed
Access of
hydrogen - radicals to the peptide - backbone as a measure for estimating the flexibility of
proteins using matrix - assisted laser desorption / ionization mass spectrometry . -
pubmed
The MALDI - ISD spectra of
bovine serum albumin (
BSA ) ,
myoglobin and
thioredoxin show discontinuous intense ion peaks originating from one - side preferential cleavage at the
N - Calpha bond of
Xxx - Asp ,
Xxx - Asn ,
Xxx - Cys and
Gly - Xxx residues . -
pubmed
RESULTS : The unstimulated salivary flow rate measured by
MST at the end of 3rd minute was 13.7 + - 10.08 , 19.86 + - 8.95 and 31.0 + - 5.4 mm and by spitting method was 0.12 + - 0.07 , 0.19 + - 0.10 and 0.30 + - 0.75 ml / min in
TCA , SSRI and controls respectively ( p<0.001 ) . -
pubmed
AIMS : A high consumption of
fructose leads not only to peripheral changes in
insulin sensitivity and vascular function , but also to central changes in several brain regions . -
pubmed
Given the role of the endogenous cannabinoid system in the control of energy intake , we undertook a pilot study to determine whether a high
fructose diet produced changes in brain
CB1 receptor functionality . -
pubmed
CB1 receptor functionality was measured autoradiographically as the increase in
[ ( 35 ) S ] GTPgammaS binding produced by the agonist
CP55,940 . -
pubmed
Two - way robust Wilcoxon analyses for each brain region indicated that the dietary treatment did not produce significant main effects upon agonist - stimulated
[ ( 35 ) S ] GTPgammaS binding in any of the regions , in contrast to a significant main effect upon both
leptin and
adiponectin levels in the blood . -
pubmed
However , a MANCOVA of the data controlling for
leptin and
adiponectin as co - variables identified a significant effect of
glucose and
fructose treatment for five weeks upon the
[ ( 35 ) S ] GTPgammaS response in the ventromedial hypothalamus , a region of importance for regulation of appetite . -
pubmed
Photoperiod treatment ( either SD or
LD ) failed to induce significant changes in the HPG activity , as measured by
androgen levels and gonadosomatic index . -
pubmed
However ,
SD increased the intensity of
aggressive behaviors and shortened the time to settle a dominance hierarchy in an
androgen - independent manner . -
pubmed
The higher
androgen response to a social challenge in residents under SD may be explained by the time course of the
androgen response that due to the long time it takes to fight resolution under
LD , might have been delayed . -
pubmed
A novel method combining Inductively Coupled Mass Spectrometry ( ICP -
MS ) with solid - phase extraction ( SPE ) to separate and purify
radium isotopes from the matrix elements in high salinity solutions is developed in this study . -
pubmed
Radium separation is accomplished using a combination of a strong - acid cation exchange resin to separate
barium and
radium from other ions in the solution and a
strontium - specific resin to isolate
radium from
barium and obtain a sample suitable for analysis by ICP -
MS . -
pubmed
Method optimization achieved high radium recovery ( 101 + - 6 % for standard mode and 97 + - 7 % for collision mode ) for synthetic Marcellus Shale wastewater ( MSW ) samples with total dissolved solids as high as 171,000 mg / L.
Ra-226 concentration in actual MSW samples with TDS as high as 415,000 mg / L measured using ICP -
MS matched very well with the results from gamma spectrometry . -
pubmed
Due to the inherent heterogeneity of conjugation to the multiple
lysines or
cysteines found in
mAbs , significant research efforts are now being directed toward the production of discrete , homogeneous ADC products , via site - specific conjugation . -
pubmed
These site - specific conjugations may involve genetic engineering of the
mAb to introduce discrete , available
cysteines or non - natural
amino acids with an orthogonally - reactive functional group handle such as an
aldehyde ,
ketone ,
azido , or
alkynyl tag . -
pubmed
As a consequence of profound acid suppression ,
serum gastrin during
vonoprazan therapy rose to much higher levels than those during
lansoprazole therapy , pointing to a requirement for the assurance of long - term safety of
vonoprazan . -
pubmed
We report a case of multiple lung
metastases of an
epithelioid sarcoma originating in the inguinal area that we treated with the
multikinase inhibitor
pazopanib . -
pubmed
The results revealed that
siomycin A induced apoptosis by influencing the downstream events of
FoxM1 , including inhibiting the expression of
Bcl-2 and
Mcl-1 , as well as leading to
caspase-3 cleavage . -
pubmed
Successful treatment of
FKBP51 - expressed
multicentric reticulohistiocytosis using combination therapy with low - dose
denosumab and
tacrolimus . -
pubmed
We identified dozens of proteins that responded to
zinc in a
ColR - independent manner , among others , known metal efflux systems
CzcCBA1 ,
CzcCBA2 ,
CadA2 and
CzcD. -
pubmed
Despite the relatively modest
ColR - dependent changes of wild - type ,
colR deficiency resulted in drastic proteome alterations , with 122 proteins up- and 62 down - regulated by
zinc . -
pubmed
This response likely alleviates the
zinc stress , as the
AlgU - regulated alginate regulator
AmrZ was shown to contribute to
zinc tolerance of
colR knockout . -
pubmed
Thus , the
ColRS system is important for
zinc homeostasis , and in its absence , alternative stress response pathways are activated to support the
zinc tolerance . -
pubmed
BX-795 is an inhibitor of
3 - phosphoinositide - dependent kinase 1 (
PDK1 ) , but also a potent inhibitor of the
IKK - related kinase ,
TANKbinding kinase 1 (
TBK1 ) and
IKKe . -
pubmed
BX-795 treatment dose - dependently suppressed the expression of two HSV immediate - early ( IE ) genes (
ICP0 and
ICP27 ) and the late gene (
gD ) at 12 h postinfection . -
pubmed
HSV-2 infection resulted in the activation of
PI3 K and
Akt in the host cells , and
BX-795 treatment inhibited HSV-2 - induced
Akt phosphorylation and activation . -
pubmed
HSV-2 infection increased the phosphorylation of
JNK and
p38 , and reduced
ERK phosphorylation at 8 h postinfection in the host cells ;
BX-795 treatment inhibited HSV-2 - induced activation of
JNK and
p38 MAP kinase as well as the phosphorylation of
c - Jun and
ATF-2 , the downstream targets of
JNK and
p38 MAP kinase . -
pubmed
Furthermore ,
SB203580 ( a
p38 inhibitor ) or
SP600125 ( a
JNK inhibitor ) dose - dependently inhibited the viral replication in the host cells , whereas
PD98059 ( an
ERK inhibitor ) was not effective . -
pubmed
Moreover ,
BX-795 blocked
PMA - stimulated
c - Jun activation as well as HSV-2 - mediated
c - Jun nuclear translocation . -
pubmed
BX-795 dose - dependently inhibited HSV-2 ,
PMA ,
TNF - alpha - stimulated
AP-1 activation , but not HSV - induced
NF - kappaB activation . -
pubmed
Overexpression of
p38 /
JNK attenuated the inhibitory effect of
BX-795 on HSV replication . -
pubmed
BX-795 completely blocked HSV-2 - induced
MKK4 phosphorylation , suggesting that
BX-795 acting upstream of
JNK and
p38 MAP kinase . -
pubmed
In conclusion , this study identifies the anti - HSV activity of
BX-795 and its targeting of the
JNK /
p38 MAP kinase pathways in host cells . -
pubmed
The deduced
PoVtn protein is comprised of 438
amino acids with a 19 - amino - acid signal peptide sequence ( 1Met-19Ala ) at the N - terminus . -
pubmed
The unique properties of the SPIONs - core and the functional gold (
Au)-shell together ( SPIONS /
Au core / shell or
CS ) have a wide range of biomedical applications including , but not limited to , magnetic resonance imaging ( MRI ) , dual modality MRI / computed tomography (
CT ) , photo - induced and magnetic fluid
hyperthermia ( MFH ) , drug delivery , biosensors , and bio - separation . -
pubmed
Herein , we report synthesis and evaluation of new twenty six small molecules against
beta amyloid (
Abeta)-induced opening of
mitochondrial permeability transition pore ( mPTP ) using
JC-1 assay which measures the change of mitochondrial membrane potential ( DeltaPsim ) . -
pubmed
The neuroprotective effect of seventeen compounds against
Abeta - induced mPTP opening was superior to that of the standard
Cyclosporin A (
CsA ) . -
pubmed
Fifteen derivatives eliciting increased green to red fluorescence percentage less than 40.0 % were evaluated for their impact on
ATP production , cell viability and neuroprotection against
Abeta - induced neuronal cell
death . -
pubmed
A method for rapid screening of
fipronil and its metabolites in egg and egg products was developed by liquid chromatography coupled with quadrupole time - of - flight mass spectrometry ( LC - QTOF
MS ) . -
pubmed
The separation was performed on a Poroshell 120 EC
C18 column ( 150 mmx3 mm , 2.7 mum ) with gradient elution using water and
acetonitrile as mobile phases . -
pubmed
Blood samples were collected from eight birds per treatment at week 4 ( starter phase ) and week 8 ( finisher phase ) of the experiment for the determination of plasma
3,5,3 '- triiodothyronine ( T3 ) ,
superoxide dismutase (
SOD ) ,
malondialdehyde (
MDA ) . -
pubmed
Gadolinium - enhanced magnetic resonance imaging
T1 - weighted sequences have been demonstrated to be adequate for this purpose . -
pubmed
Note that we used the Medtronic StealthStation
S8 ( Minneapolis , Minnesota , USA ) and
gadolinium - enhanced magnetic resonance imaging
T1 - weighted sequences to plan 4 different surgical procedures ( needle biopsy , parasagittal
meningioma , double
metastases , and high - grade
glioma ) . -
pubmed
Proteobacteria were more abundant in the
AT group than in the
MST group and may include denitrifying bacteria contributing to
nitrous oxide (
N2O ) emission or aerobic bacteria stimulated by
oxygen . -
pubmed
We also investigated the clinical relevance of an
OqxR loss - of - function mutation , particularly in the context of
beta - lactam susceptibility . -
pubmed
RamR loss activated
AcrAB efflux pump production ~5 - fold and this reduced
beta - lactam susceptibility , conferring
ertapenem non - susceptibility even in the absence of a carbapenemase . -
pubmed
This reduced
fluoroquinolone susceptibility but had little impact on
beta - lactam susceptibility even in the presence of a
beta - lactamase . -
pubmed
This means that only
RamR mutants have significantly reduced
beta - lactamase - mediated
beta - lactam susceptibility and therefore represent a greater clinical threat . -
pubmed
Results : Twenty - four weeks of exposure to smokeless tobacco products or
nicotine significantly decreased the levels of circulating
Flk+/
Sca+ endothelial progenitor cells . -
pubmed
Neither smokeless products nor
nicotine affected plasma lipoproteins , platelet activation , or systemic
insulin sensitivity . -
pubmed
Increased formation of proinflammatory
cytokines such as
TNFalpha by
nicotine or Copenhagen snuff may lead to
vascular inflammation and thereby exacerbate
atherogenesis . -
pubmed
We hypothesized that
trauma experience would have a negative impact on brain function , resulting in altered basal
cortisol levels via dysregulated neural control over the
HPA axis system . -
pubmed
Molybdenum carbides and
nitrides preferentially activate C O and
C - OH bonds over
C C and
C - C bonds in liquid - phase hydrogenation of bioderived
furfural , leading to highly selective formations of
furfuryl alcohol (
FA ) and its subsequent hydrogenolysis product (
2 - methyl furan ( 2 - MF ) ) . -
pubmed
, & gt ; 90 % yields of
FA in
methanol solvent and of 2 - MF in
2 - butanol solvent at 423 K ) . -
pubmed
Investigating the Effect of Demographics , Clinical Characteristics , and Polymorphism of
MDR-1 ,
CYP1A2 ,
CYP3A4 , and
CYP3A5 on
Clopidogrel Resistance . -
pubmed
However , known polymorphisms of
MDR-1 ,
CYP1A2 ,
CYP3A4 , and
CYP3A5 were not associated with treatment resistance when compared to responders to
clopidogrel therapy . -
pubmed
My early studies focused on understanding how
insulin promotes
glucose transport into adipocytes , a classic model of highly
insulin - responsive target cells . -
pubmed
We found that the
AMP - activated protein kinase (
AMPK ) pathway mediates
leptin 's effects on
fatty acid oxidation in muscle and also plays a role in
leptin 's anorexigenic effects in the hypothalamus . -
pubmed
Cell viability was determined using
XTT assay and was assessed at 0 , 24 and 48 h intervals after a 10 - min - long treatment , followed by thorough
washes , or at 24 and 48 h of treatment at
TXA concentrations of 20 , 50 , 70 and 100 mg / ml . -
pubmed
Valproic acid (
VPA ) is a
histone deacetylase (
HDAC ) inhibitor that has shown positive results on SMA both in experimental and cohort studies . -
pubmed
The primary outcome was the efficacy of
VPA in terms of gross motor function and expression of both full - length spinal motor neuron (
SMN ) gene (
FL -
SMN ) and exon 7 - lacking
SMN . -
pubmed
The overall effect estimate , comparing pre- and
post -
VPA treatment , regardless of
carnitine co - administration and design of the studies , showed significant improvement in gross motor function ( standard mean difference [ SMD ] = 0.302 , 95 % confidence interval [ CI ] 0.048 - 0.556 , P = 0.02 ) using the Hammersmith Functional Motor Scale ( HFMS ) , Modified Hammersmith Functional Motor Scale ( MHFMS ) , and MHFMS - Extend , with no significant heterogeneity . -
pubmed
No significant differences were found between pre- and
post -
VPA treatment co - administered with
carnitine , in terms of the change in FL -
SMN and exon 7 - lacking
SMN . -
pubmed
In conclusion ,
farnesol has antiarrhythmic effect mediated by reducing of ICa , L and
IK along with a decrease of reactive
oxygen species production and normalized
Ca2 + sparks and waves . -
pubmed
Azoramide is identified as a new compound with the dual properties for the improvement of
ER - folding capacity in various cells as well as for the treatment of
T2DM . -
pubmed
Therefore , here , we aimed to investigate the direct effect of
azoramide on
insulin signaling in
insulin - resistant ( IR ) cardiomyocytes using IR - modelled ventricular cardiomyocytes . -
pubmed
This work shows that , when the electron - donating ability of the N - substitution is further increased in the presence of a
carbanion or
phenoxide , spontaneous electron transfer (
ET ) occurs and results in radical anions , verified with electron - paramagnetic resonance (
EPR ) spectroscopy . -
pubmed
We analyzed the photoantimicrobial effect caused by red light activation of
methylene blue (
MB - APDT ) and by blue light ( BL ) activation of endogenous microbial pigments against 5 clinically relevant pathogens . -
pubmed
Durable Response of Low - Dose
Afatinib plus Cetuximab in an
Adenocarcinoma Patient with a Novel
EGFR Exon 20 Insertion Mutation . -
pubmed
Orientin suppresses oxidized
low - density lipoproteins induced
inflammation and oxidative stress of macrophages in
atherosclerosis . -
pubmed
The scavenger receptor
CD 36 of
ox - LDL was significantly downregulated after the treatment of
orientin . -
pubmed
Inhibition of ROS generation and increasing of
eNOS expression by
Orientin treatment was used to show the alteration of oxidative stress . -
pubmed
Moreover , the expression levels of
Angiopoietin - like 2 (
angptl2 ) and
NF - kappaB were significantly upregulated after cells induced by ox - LDL , whereas
orientin significantly reversed the effects of
ox - LDL . -
pubmed
Orientin inhibited ox - LDL - induced
inflammation and oxidative stress , and
CD36 may be the key regulator during
Orientin action . -
pubmed
The mRNA levels of genes involved in
carbon fixation , namely ,
rbcL and
rbcS , were clearly increased compared with those under shelter - free conditions , resulting in improved
CO2 utilization . -
pubmed
Furthermore , the transcription levels of genes involved in
chlorophyll (
hemA ,
hemN , and
chlH ) and carotenoid synthesis (
crtB ,
PDS ,
crtISO , and
lcyB ) in the sheltered leaves peaked earlier than those in the unsheltered leaves , thereby promoting organic matter accumulation in leaves . -
pubmed
Remarkably , the expression levels of key genes involved in the metabolic pathways of
phenylpropanoid (
PAL ,
C4H , and
4CL ) and
flavonoid (
CHS ,
CHI , F3'H , DFR , and ANS ) in the sheltered fruits were also up - regulated earlier than of those in the unsheltered fruits , conducive to an increase in
anthocyanin content in the fruits . -
pubmed
Particularly ,
ibutamoren , known as an agonist of
growth hormone secretagogue receptor (
GHSR ) , showed the most promising antiviral activity with a 50 % effective concentration of 0.2 muM , a 50 % cytotoxic concentration of 42.4 muM , and a selectivity index of 222.8 . -
pubmed
Although
sFlt-1 is a soluble protein , it contains a binding site for the extracellular matrix component
heparan sulfate . -
pubmed
Consequently , the placenta becomes a potential storage site for large amounts of
sFlt-1 bound to extracellular
heparan sulfate . -
pubmed
Additionally , it should be noted that
sFlt-1 can bind to the anticoagulant unfractionated heparin due to its molecular mimicry to
heparan sulfate . -
pubmed
However , it remains unknown whether unfractionated heparin can compete with
heparan sulfate for binding of localized
sFlt-1 . -
pubmed
Real - time quantitative PCR of the explants showed no change in
sFlt-1 or
heparanase-1 mRNA expression , eliminating increased production and enzymatic cleavage of
heparan sulfate as causes for
sFlt-1 media increase . -
pubmed
These data demonstrate that chronic unfractionated
heparin treatment is able to displace matrix - bound
sFlt-1 into the maternal circulation to such a degree that mean arterial pressure is significantly affected . -
pubmed
MEDI0562 , an agonistic humanized monoclonal antibody ( mAb ) , specifically binds to the
costimulatory molecule OX40 . -
pubmed
MEDI0562 induced increased
Ki67 +
CD4 + and
CD8 + memory T - cell proliferation in the periphery and decreased intratumoral
OX40 +
FOXP3 + cells . -
pubmed
During a follow - up visit , she showed signs of acute exacerbation and she was switched to
benralizumab during her acute phase with a rapid , dramatic amelioration of respiratory symptoms and
pulmonary function , without concomitant systemic
corticosteroid administration . -
pubmed
In potentiostatic electrolysis of 50 mM
NaCl solutions , dramatic improvement ( 0.61 mmol cm-2 hr-1 at 2.5 V
NHE ) was noted by simple coating of thin ( ~2 mum )
TiO2 layer from ball - milled
TiO2 nanoparticle ( 80 - 100 nm ) suspension , even with moderate elevation in voltammetric wave . -
pubmed
However ,
Bi - doped
TiO2 layers prepared from
polyol - mediated or co - precipitation methods marked the highest CERCS ( ~100 % ) and EERCS ( 8.16 mmol Wh-1 at 2.5 V
NHE ) by increased mixing level and effective shift in surface charge . -
pubmed
In the present study , using 10 muM of
gibberellic acid (
GA3 ) , or
gibberellin biosynthesis (
GA ) inhibitors -
ancymidol ( A ) and
paclobutrazol ( P)-the dynamic changes in soluble
sugars ,
fructose and
glucose content , fresh weight and sprouting capacity were investigated . -
pubmed
meleagris bulbs were cultured on medium with
GA3 and
GA inhibitors for 1 , 2 and 5 weeks at two different temperatures ( 24 and 7 C ) . -
pubmed
The sensitivity of bulbs to
GA inhibitors differed and significantly affected
sugar content in bulbs . -
pubmed
BCR -
ABL was assessed by quantitative rt - PCR at days 0 and 90 of
imatinib therapy . -
pubmed
The critical binding region of
ACE2 is an
~ 30 - amino - acid ( aa)-long helix . -
pubmed
NYBSP-2 and
NYBSP-4 also prevented the formation of the virus - induced
CPE with an IC100 of about 33 muM. -
pubmed
The aims of this study were ( i ) to determine whether different metabolic profiles of
B - vitamins and
vitamin A in Brazilian children and adolescents were positively or negatively related to
homocysteine and
omega-3 fatty acids using k - means clustering analysis , ( ii ) compare nutrient intakes and metabolites between the different metabolic profiles , ( iii ) evaluate if the statistically significant metabolites found between the metabolic groups , can predict the variation of
leukotriene A4 hydrolase (
LTA4H ) levels , a biomarker of
low - grade inflammation , in the total studied population . -
pubmed
Multiple linear regression analyses showed that blood
cobalamin ,
riboflavin ,
pyridoxal and
homocysteine combined , explained 9.0 % of
LTA4H levels variation in the total studied population . -
pubmed
The combined levels of the
riboflavin ,
pyridoxal ,
cobalamin and
homocysteine biomarkers can predict the variation of
LTA4H in the total population studied , but it is not clear how this regulation occurs . -
pubmed
Vibrational spectra of
H14 - Cp * Rh - Cl and
D14 - Cp * Rh - Cl were captured via inelastic neutron spectroscopy (
INS ) and assigned using density functional theory ( DFT ) phonon calculations . -
pubmed
The therapeutic potential of
TAK-242 , a selective
TLR4 inhibitor , was evaluated for OD . -
pubmed
TAK-242 treatment significantly reduced the level of
IL-1beta ,
IL-6 , and TNF - alpha mRNA expression , as well as activation of microglia and astrocytes in the
OB tissues . -
pubmed
U0126 is a specific inhibitor of the
extracellular signal - regulated protein kinases 1 and 2 (
ERK1 / 2 ) signaling pathway and has neuroprotective effects . -
pubmed
Prospective and cross - sectional studies that evaluated the association between
Hg exposure and the levels of
thyroid stimulating hormone (
TSH ) ,
triiodothyronine ( T3 ) ,
thyroxine ( T4 ) , free
triiodothyronine ( FT3 ) , and free
thyroxine ( FT4 ) were included . -
pubmed
The presence of
Hg in blood may significantly increase the levels of
TSH ( beta=0.55 ; 95%CI : 0.20,0.90 ; p < 0.001 ) and FT4 ( beta=0.47 ; 95%CI : 0.11,0.82 ; p < 0.001 ) , with the opposite association in T4 ( beta=-0.02 ; 95%CI : 0.02 , -0.01 ; p < 0.001 ) . -
pubmed
For the subgroup analysis , blood
Hg was positively correlated with
TSH levels in children and adolescents ( beta=0.62 ; 95%CI : 0.09 , 1.15 ; p < 0.001 ) and FT4 levels in pregnant women ( beta=1.00 ; 95%CI : 0.99 , 1.00 ; p < 0.001 ) respectively . -
pubmed
CONCLUSIONS : This meta - analysis indicates that exposure to
Hg in blood could significantly corrrelate with the levels of
TSH , T4 , and FT4 in the general population . -
pubmed
Inactivation of
tobacco ribulosebisphosphate carboxylase by
2,3 - butanedione . -
pubmed
A fluorometric method for the determination of
naproxen in serum ,
albumin solutions and
protein free buffer solutions is described . -
pubmed
The in vitro binding of
naproxen in
albumin solutions and serum was studied by equilibrium dialysis . -
pubmed
At proestrus , the uptake of
125I -
HCG was unaffected by the
LH surge at 18.00 h but had decreased slightly at 24.00 h. The implications of these data in relation to the regulation of receptor sites , is discussed . -
pubmed
We recorded a bioassay for
prolactin using densitometric analysis on
polyacrylamide gels of
caseins produced by mammary explants in vitro . -
pubmed
Both plasma and cerebrospinal fluid levels of
oxytocin were measured under similar conditions , following
CRF and
morphine treatments . -
pubmed
Morphine and
CRF potentiated the effects of vaginocervical stimulation on these behaviours . -
pubmed
The results show that maternal experience is essential for
oxytocin to promote proceptive behaviours and that
morphine and
CRF potentiate the effects of vaginocervical stimulation on acceptance behaviour . -
pubmed
This
wound was closed using either a sutureless tissue weld created by a microscope - mounted
CO2 laser ( 153 W / cm2 ) ( Group I , N = 61 ) or with a running 6 - O
polypropylene suture ( Group
II , N = 58 ) . -
pubmed
Fluorography after photoaffinity labeling as well as immunoblotting with a specific polyclonal antibody revealed that the
human androgen receptor migrated as a closely spaced 110 kD doublet on
SDS - polyacrylamide gels . -
pubmed
Previous studies demonstrating hydrolysis of
phosphatidylinositol bisphosphate (
PIP2 ) and generation of
inositol phosphates in neutrophils exposed to 20.0 mM
NaF provide indirect evidence that activation of
phospholipase - associated guanine nucleotide regulatory protein , a
guanine nucleotide binding protein which regulates the activation of a membrane
inositol - specific phospholipase C , is an early event in the neutrophil stimulus - response pathway triggered by
fluoride . -
pubmed
To further explore the involvement of
guanine nucleotides in functional responses of intact neutrophils triggered by
fluoride , we preincubated cells with
2 - beta - D - ribofuranosylthiazole-4 - carboxamide (
tiazofurin ) , a selective inhibitor of
inosine monophosphate dehydrogenase , to diminish
guanine nucleotide synthesis and then compared
superoxide generation induced by
FMLP ,
PMA ,
digitonin , and 20.0 mM
NaF to intracellular levels of
guanine nucleotides . -
pubmed
The effects of
tiazofurin on
guanine nucleotides levels were not observed when neutrophils were preincubated at 4 degrees C.
AT 37 degrees C ,
tiazofurin also decreased intracellular
ATP and
ADP levels but
adenine nucleotide depletion was less pronounced than
guanine nucleotide depletion for each concentration of
tiazofurin used . -
pubmed
Acute exposure by inhalation to 100 mg / m3
silica dust produced persistent
pulmonary inflammation , as well as long - term changes in pulmonary alveolar macrophages (
AM ) typical of macrophage activation . -
pubmed
After
silica inhalation , the following changes were observed constantly in bronchopulmonary lavage cell samples : increased numbers of polymorphonuclear leukocytes ( PMN ) , hypersegmented PMN , binucleated AM , increased
AM phagocytic indices and capacities , and increased mycocidal activity . -
pubmed
Exposure to 500 mg / m3
graphite produced transient
inflammation and
AM activation for about 24 - 48 hr . -
pubmed
The results of this study provide strong evidence at the level of mRNA expression that
ProEnk A mRNA is expressed preferentially in the
adrenaline synthesizing cells within the adrenal medulla . -
pubmed
This reduction was 2.06 + /- 1.8 for the control , 1.96 + /- 1.27 when
captopril (
CAP ) ( 10(-5 ) M ) was added to the perfusion liquid and 1 + /- 0.42 when
perindopril (
PER ) ( 1.2 x 10(-5 ) M ) was added to the perfusion liquid ( p less than 0.05 vs
CAP ) . -
pubmed
In the presence of
CAP or
PER the effects of
AII were the same , however in the presence of
SAR these effects were prevented . -
pubmed
This
seizure suppressant action was dose dependent , spatially specific for the area of the
SN and was not blocked by
naloxone ( 2 mg / kg i.p . ) -
pubmed
Chemical modification of
aphidicolin and the inhibitory effects of its derivatives on
DNA polymerase alpha in vitro . -
pubmed
In the presence of 0.1 %
Triton X-100 extensive degradation of the receptor by
trypsin is observed . -
pubmed
Assays of extracts from the lapel filters for
Cat-1 content by radioimmunoassay and for allergenic activity by leukocyte
histamine release demonstrated a significant correlation ( p less than 0.05 ) between the results of the two assays . -
pubmed
The determination of the dissociation velocity constants of
rabbit uterine cytosol receptor -
oestrogen complexes . -
pubmed
The first
kinase , present in greatest activity in microsomal extracts , appears to be identical to
casein kinase I by characteristic molecular size on gel filtration ( Mr 40,000 ) and
sodium dodecyl sulfate - gel electrophoresis ( Mr 34,000 ) , autophosphorylation of this single subunit , inability to efficiently utilize
GTP , and resistance to inhibition by heparin and
2,3 - diphosphoglycerate . -
pubmed
The second
kinase , predominant in cytosol , appears to be identical to
casein kinase II by characteristic molecular size on gel filtration ( Mr 150,000 ) , an autophosphorylated subunit of Mr 25,000 , a Km for
GTP nearly equal to that of
ATP , inhibition by heparin and
2,3 DPG , and relative substrate specificity . -
pubmed
Despite the incorporation of up to 2 mol
32P / mol carboxylase subunit (
kinase I ) and 0.6 mol / subunit (
kinase II ) , phosphorylation by either
kinase causes no change in
carboxylase activity . -
pubmed
The site(s ) phosphorylated by each
kinase and by the
cAMP - dependent protein kinase on
carboxylase appear to be clustered on a Mr 16,000
cyanogen bromide peptide that is readily released on incubation with
trypsin . -
pubmed
Preparation of a biocompatible
albumin - coated ion exchange resin for
bilirubin removal from the blood of jaundiced newborns . -
pubmed
The choice of the appropriate ionic form of the resin and the proper
albumin coating and crosslinking procedure assures a high
bilirubin adsorption capacity and excellent blood compatibility of the resin . -
pubmed
The
albumin coated resin removes in vitro 80 - 90 % of the
bilirubin initially present in the plasma . -
pubmed
After treatment with
clomiphene and
HCG in 16 women one or more oocytes were harvested . -
pubmed
The results of this study are as follows : FFA was significantly lower , while
pyruvate was significantly higher at
rest after a high
CHO diet than after a low
CHO diet ( P less than 0.05 ) . -
pubmed
The cells were cryopreserved and stored under liquid
nitrogen for intervals of 2 , 4 , 6 , 8 , or 12 wk , then assayed for viability ( using the
trypan dye exclusion method as well as the
NADH - dependent cytochrome c reductase (
cytochrome c ) assay ) and cellular function ( as documented by the measurement of in vitro
aryl hydrocarbon hydroxylase (
AHH ) induction by
benzanthracene ( BA ) and by quantitation of
PAM particulate phagocytosis ) . -
pubmed
Similarly , when
AHH induction was fluorometrically quantitated in
PAM cultured for 24 h , there was no decrease in BA - induced
AHH levels after a 2 - wk cryopreservation period . -
pubmed
However ,
AHH levels decreased slightly compared with those in the 0 - time control cells ( p less than 0.004 ) , when
PAM were stored for longer intervals of 4 , 6 , or 8 wk . -
pubmed
The purified
enzyme was free of other known brush border enzymes and appeared homogeneous in immunoelectrophoresis and
polyacrylamide gel electrophoresis in the presence of
SDS . -
pubmed
Increased
thyrotrophin and
prolactin secretion induced by
domperidone in
hypothyroid subjects . -
pubmed
Domperidone ( 4 mg iv ) induced a clear - cut and sustained rise in both plasma
TSH and
Prl , with peak levels occurring 15 and 30 min
post - injection , respectively . -
pubmed
The increments in plasma
Prl after
domperidone were significantly higher than those in
plasma TSH . -
pubmed
Clomipramine - induced
agranulocytosis and its treatment with
G - CSF . -
pubmed
When
prolactin and
growth hormone were coinjected ,
growth hormone also proved unable to oppose the
Na+ retaining effect of
prolactin , in both brackish and fresh water . -
pubmed
Here , to test whether beta 2 carboxyl sequences can rescue the functional failure of the
beta 3 isoform in spermatogenesis , we constructed a gene encoding a chimeric protein ,
beta 3 beta 2C , in which beta 3 sequences in the
carboxyl region are replaced with those of
beta 2 . -
pubmed
Truncated forms of
gC and
gD ,
gC1(457 t ) ,
gC2(426 t ) , and
gD1(306 t ) , lacking the transmembrane and
carboxyl regions were expressed in the baculovirus system . -
pubmed
Losartan , nonpeptide
angiotensin II - type 1 ( AT1 ) receptor antagonist , attenuates pressor and sympathoexcitatory responses evoked by
angiotensin II and
L - glutamate in rostral ventrolateral medulla . -
pubmed
We investigated the effect of
losartan , a nonpeptide
angiotensin II ( Ang II )- type 1 ( AT1 ) receptor antagonist , on the responses evoked by
Ang II and
L - glutamate (
L - Glu ) in the rostral ventrolateral medulla ( RVLM ) . -
pubmed
Responses of mean arterial pressure ( MAP ) , heart rate ( HR ) and splanchnic sympathetic nerve activity ( SNA ) to microinjection of
Ang II ( 100 pmol ) or
L - Glu ( 2 nmol ) into the RVLM were examined following microinjection of
losartan ( 10 pmol-10 nmol ) . -
pubmed
Ang II increased MAP ( 16 + /- 1 mmHg in SHR and 16 + /- 1 mmHg in WKY ) and SNA ( 9 + /- 1 % and 10 + /- 1 % , respectively ) , which were significantly ( P & lt ; 0.01 ) attenuated by pretreatment with
losartan ( 100 pmol-10 nmol ) in both
strains . -
pubmed
Reconstitution of the activity of dialyzed
arginase by
manganese is a two - phase process ; the second phase is independent of the cation concentration , with a half - time recovery ( t1/2 ) of 10.77 min . -
pubmed
The apparent Km for
Mn2 + is 280 microM and 10.5 microM for
enzyme dialyzed for 24 and 72 hr , respectively . -
pubmed
Treatment with 5 mM
EDTA at pH 6 totally inhibits
enzyme activity , which is reconstituted by
Mn2 + . - pubmed
Results obtained with
iodoacetamide treatment suggest the existence of
sulphydryl groups accessible only when the
enzyme is dialyzed . -
pubmed
Significant increases in
superoxide dismutase (
SOD ) ,
glutathione peroxidase and
catalase (
CAT ) activities were observed at 15 or 18 hours after the injection of
paraquat . -
pubmed
In the lung , lipid
peroxide concentration decreased significantly and the activities of
SOD and
CAT increased at 15 hours after injection of
paraquat . -
pubmed
However , the
enzyme induction was produced by
paraquat without the increase of TBARS in the lung . -
pubmed
During endogenous feeding , despite larval drinking ,
MT mRNA levels did not appear to be inducible by exposure to
Cd in the water within 3 days of hatching but were induced three- to fivefold after 48 hr exposure to waterborne
Cd at all later stages of development . -
pubmed
The results suggest that
Cd tolerance is associated with metal induction of
MT gene transcription and that the enhanced sensitivity of very early stage larvae is attributable to a deficiency in MT gene transcription . -
pubmed
Blockade of DNA synthesis induced by
platelet - derived growth factor by
tranilast , an inhibitor of
calcium entry , in vascular smooth muscle cells . -
pubmed
At lower concentrations ,
PDGF induced oscillatory changes in [
Ca2 +]c , and reduction of extracellular
calcium attenuated the oscillation . -
pubmed
An antiallergic compound ,
tranilast , abolished the sustained phase of [
Ca2 +]c induced by 1 nM
PDGF . -
pubmed
Tranilast also inhibited the oscillatory changes in [
Ca2 +]c induced by 200 pM
PDGF . -
pubmed
In addition ,
PDGF - induced
calcium influx in the late
G1 phase , as assessed by measuring the initial uptake of
45Ca , was inhibited by
tranilast in a concentration - dependent manner . -
pubmed
Tranilast also inhibited
PDGF - augmented DNA synthesis ; the ID50 for the inhibition of DNA synthesis was nearly identical to that for
calcium influx . -
pubmed
Although
tranilast blocked
PDGF - induced
calcium entry , it did not affect
PDGF - mediated autophosphorylation of the
PDGF receptor , activation of
phosphatidylinositol 3 - kinase , activation of
Ras or
mitogen - activated protein kinase . -
pubmed
Similarly ,
PDGF - induced elevation of
diacylglycerol was not affected by
tranilast . -
pubmed
These results suggest that the antiallergic drug
tranilast inhibits
PDGF - induced DNA synthesis by blocking
PDGF - mediated
calcium entry . -
pubmed
Tranilast may be of use in controlling
PDGF - induced DNA synthesis in VSMC . -
pubmed
Surface damage of the tibial plateau components of knee prostheses made from medical grade ultra - high - molecular - weight
polyethylene ( UHMW -
PE ) has been attributed to delamination wear caused by a
fatigue fracture mechanism . -
pubmed
Gelsolin is a
calcium - activated actin - binding protein with six subdomains . -
pubmed
A
Ca2 + -sensitive truncate containing
G2 - 6 inhibited the
F - actin - depolymerizing activities of both
gelsolin and
cofilin , while a
G2 - 3 truncate was less effective . -
pubmed
Cosedimentation assays in the presence of
calcium demonstrate that the
G2 - 6 truncate binds to
F - actin more strongly than the
G2 - 3 truncate consistent with a protection mechanism by conformational change of
F - actin and/or competitive binding to
actin filaments which depends upon the presence of
actin filament binding domains . -
pubmed
Acting through
cell surface receptors ,
ADP activates platelets resulting in shape change , aggregation ,
thromboxane A2 production , and release of granule contents . -
pubmed
ADP also causes a number of intracellular events including inhibition of
adenylyl cyclase , mobilization of
calcium from intracellular stores , and rapid
calcium influx in platelets . -
pubmed
The receptor responsible for the actions of
ADP on platelets has been designated the
P2 T receptor . -
pubmed
In this study we have used
ARL 66096 , a potent antagonist of
ADP - induced
platelet aggregation , and a
P2X ionotropic receptor agonist ,
alpha , beta - methylene adenosine 5 '- triphosphate , to distinguish the
ADP - induced intracellular events . -
pubmed
ARL 66096 blocked
ADP - induced inhibition of
adenylyl cyclase , but did not affect
ADP - mediated intracellular
calcium increases or shape change . -
pubmed
These results clearly demonstrate the presence of two distinct platelet
ADP receptors in addition to the
P2X receptor : one coupled to
adenylyl cyclase and the other coupled to mobilization of
calcium from intracellular stores through
inositol trisphosphates . -
pubmed
The synthetic 20 base
oligoribonucleotide , which consists of the template sequence CUAACCCUAAC and the adjacent region of the RNA component of
human telomerase , was also found to form the complex with the recombinant
nm23 -
H2 protein . -
pubmed
However , at & gt ; 25 micrograms / cm2
MnSOD mRNA levels in
silica- and
crocidolite- but not in rockwool - exposed cells decreased . -
pubmed
These results suggest that : ( 1 ) like
crocidolite and
silica , rockwool accelerates
MnSOD gene expression in bronchoepithelial cells ; ( 2 ) an increase of
MnSOD mRNA levels is not accompanied by
MnSOD activity elevation ; ( 3 ) in contrast to rockwool , high concentrations ( & gt ; or = 25 micrograms / cm2 ) of
crocidolite and
silica reduced
MnSOD activity and
MnSOD mRNA levels . -
pubmed
Because oxidants (
H2O2 ) and
crocidolite fibers were shown to reduce
SOD activity , lack of active
MnSOD protein may be caused by inactivation on a
post - translational level . -
pubmed
The
fibronectin synthesis increased 2.3- and 3.9 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of
saponin , respectively , compared with that in non - treated cells . -
pubmed
Fibronectin synthesis stimulated with 10 microg ml(-1 ) of
saponin was inhibited with 69 % by 5 microg ml(-1 ) of an anti -
TGF - beta1 antibody . -
pubmed
mRNA of
TGF - beta type I receptor increased 4.8- and 4.4 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of
saponin , respectively , and that of
TGF - beta type II receptor also increased 3.4- and 3.2 - fold at fibroblasts treated with 1 and 10 microg ml(-1 ) of
saponin , respectively . -
pubmed
The significant increases of
TGF - beta type I and II receptors and of
fibronectin synthesis were observed at the same concentrations of
saponin . -
pubmed
TGF - beta content increased 1.74- and 1.87 - fold at conditioned medium of fibroblasts treated with 100 and 250 microg ml(-1 ) of
saponin , respectively , higher concentrations than those which accelerated
fibronectin synthesis . -
pubmed
Furthermore , the active
TGF - beta content was below 10 % of total
TGF - beta at each concentration of
saponin . -
pubmed
These results indicate that
saponin stimulates
fibronectin synthesis through the changes of
TGF - beta receptor expressions in fibroblasts . -
pubmed
The enzyme exhibited the glucan transfer and
Glc production activities that define
D - enzymes . -
pubmed
D - enzyme also transferred glucans among the outer chains of amylopectin ( using the polysaccharide chains as both donor and acceptor ) and from
malto - oligosaccharides into the outer chains of either amylopectin or glycogen . -
pubmed
Absence of all three activities co - segregated with the
sta11 - 1 mutation , which is known to cause abnormal accumulation of
oligosaccharides at the expense of starch . -
pubmed
We surmised that the new
Cys was most probably cysteinylated as this
albumin species would have a mass decrease of 742 Da and be very close to the measured value of 755 Da . -
pubmed
Cysteinylation was confirmed when a mass decrease of 863 Da was measured between the
proteins after reduction of their
disulfide bonds . -
pubmed
Previous studies have demonstrated a positive or negative P - wave configuration in lead aVL originating from this area and a change from a biphasic P - wave in
V1 during
sinus rhythm to a positive P - wave configuration during
tachycardia . -
pubmed
Experimental results from an isothermal laminar flow reactor at atmospheric pressure are presented on the chemical composition in the
post - oxidative region of two sooting fuel - rich pre - mixed mixtures diluted in
nitrogen . -
pubmed
Signal transduction inhibitor (
STI571 ) ( formerly CGP57 148B ) , is an
ABL specific ,
tyrosine kinase inhibitor . -
pubmed
Divalent metal transporter 1 (
DMT1 ) is the major
transferrin - independent
iron uptake system at the apical
pole of intestinal cells , but it may also transport
iron across the membrane of acidified endosomes in peripheral tissues . -
pubmed
Iron transport and expression of the 2 isoforms of
DMT1 was studied in erythroid cells that consume large quantities of
iron for biosynthesis of
hemoglobin . -
pubmed
Interestingly ,
iron release from
transferrin inside the endosome is normal in mk / mk reticulocytes , suggesting a subsequent defect in
Fe (++ ) transport across the endosomal membrane . -
pubmed
Experiments with isoform - specific anti -
DMT1 antiserum strongly suggest that it is the non -
iron - response element containing isoform
II of
DMT1 that is predominantly expressed by the erythroid cells . -
pubmed
Together , these results provide further evidence that
DMT1 plays a central role in
iron acquisition via the
transferrin cycle in erythroid cells . -
pubmed
Efficient one -
pot synthesis of the
2 - aminocarbonylpyrrolidin-4 - ylthio - containing side chain of the new broad - spectrum
carbapenem antibiotic
ertapenem . -
pubmed
Starting material
N -( O , O - diisopropyl phosphoryl )- trans-4 - hydroxy - L - proline is converted in a one -
pot process to
( 2S )- cis-3 -[[( 4 - mercapto-2 - pyrrolidinyl ) carbonyl ] amino ] benzoic acid monohydrochloride in 70 - 75 % overall yield via a series of six reactions . -
pubmed
Photoconsumption of
oxygen in
photosystem II preparations under impairment of the water - oxidizing complex . -
pubmed
Oxygen consumption in
photosystem II (
PSII ) preparations in the light was 2 micromol
O2 / h per mg
Chl at weakly acidic and at neutral pH values . -
pubmed
The addition of either artificial electron donors for PSII such as
MnCl2 or
diphenylcarbazide , or
diuron as an inhibitor of electron transfer from QA , the primary bound
quinone acceptor , to QB , the secondary bound
quinone acceptor of
PSII , resulted in a decrease in
oxygen consumption rate at basic pH to value close to ones measured at pH 6.5 . -
pubmed
In experiments with
PSII preparations treated with
Tris to destroy the water - oxidizing complex , photoconsumption of
oxygen in the entire pH region was close to the values in untreated preparations at basic pH. -
pubmed
In untreated preparations the rate of light - induced
oxygen consumption decreased in the presence of
catalase , which decomposes
H2O2 , as well as in the presence of electron acceptor
potassium ferricyanide . -
pubmed
From these data it is suggested that the light - induced
oxygen consumption in
PSII is caused by two processes , by an interaction of
O2 with organic radicals , which were formed due to oxidation of components of the donor side of this
photosystem ( proteins , lipids , pigments ) by cation - radical
P680 + , as well as by
oxygen reduction by still unidentified components of
PSII . -
pubmed
A site of
ERK - dependent phosphorylation on
Smad2 was located to
Thr(8 ) , a site that overlaps with the calmodulin binding region . -
pubmed
We show that
calmodulin inhibits
Smad2 phosphorylation by
ERK1 , and overexpressing
calmodulin , or stimulating
calmodulin activity with
ionomycin , reduces
Smad2 levels . -
pubmed
Interestingly , one known
IgE - binding epitope of
Ara h 3 shares 67 % of homologous
amino acid residues with the corresponding area of
Cor a 9 . -
pubmed
The
amino acids that differ were previously shown not to be critical for
IgE binding in Ara h 3 . -
pubmed
Triterpenes and
phytosterols as
human leucocyte elastase inhibitors . -
pubmed
Ten
triterpenes and
phytosterols beta - amyrin ,
lupeol ,
lupeol acetate ,
ursolic acid ,
friedelin ,
canophyllol ,
29 - hydroxy - friedelan-3 - one ,
beta - sitosterol ,
3 - O - beta - D - glucopyranosyl - beta - sitosterol ,
3 - O -( 6 '- O - palmitoyl )- beta - D - glucopyranosyl - beta - sitosterol , were evaluated as potential inhibitors of
human leucocyte elastase (
HLE ) . -
pubmed
In this series ,
lupeol ,
ursolic acid and
canophyllol showed marked
HLE inhibitory activity with IC(50 ) values at 1.9 microM , 4.4 microM , and 2.5 microM , respectively . -
pubmed
It appeared that
HLE inhibition depended on the presence and the orientation of two reactive groups in the tested molecules , distant from 10 - 12 A , reacting with
Arg-217 in S(4 ) -S(5 ) subsites of the extended substrate - binding domain of
HLE , and S(3 ) , respectively . -
pubmed
The significantly higher level of
cholesterol in aging neuronal membrane , the slow rate of
cholesterol turnover , and the decreased level of total
polyunsaturated fatty acids (
PUFA ) may result from poor passage rate via the blood - brain barrier , or from a decreased rate of incorporation into the membrane , or a decrease in the activities of
delta-6 and delta-9 desaturase enzymes . -
pubmed
FGF2 affords neuroprotection by interfering with a number of signaling pathways , including expression and gating of
NMDA receptors , maintenance of
Ca2 + homeostasis and regulation of ROS detoxifying enzymes . -
pubmed
This stable cell line was validated with known
CYP1A1 inducers , such as
3 - methylcholanthrene (
3 - MC ) ,
beta - naphthoflavone (
beta - NF ) ,
alpha - naphthoflavone (
alpha - NF ) and
3 - indocarbinol . -
pubmed
RESULTS :
CYP1A1 reporter gene system can be used to identify strong inducers , such as
3 - MC , beta - NF and alpha - NF , and weak inducers , such as
3 - indocarbinol . -
pubmed
Involvement of
sulfoquinovosyl diacylglycerol in the structural integrity and heat - tolerance of
photosystem II . -
pubmed
The
PSII core complex of
hf-2 , as compared with that of the wild type , showed structural fragility when solubilized with a detergent ,
dodecyl beta - d - maltoside , suggesting that the physical properties of the
PSII complex were altered by the loss of
SQDG . -
pubmed
On the other hand , exposure of the cells to 41 degrees C for 120 min in the dark decreased the
PSII activity to 70 % and 50 % of the initial levels in the wild type and
hf-2 , respectively , which implies that the
PSII activity , in the absence of
SQDG , becomes less stable under heat - stress conditions . -
pubmed
These results suggest that
PSII inactivated by heat recovers through some mechanism dependent on light , and that
SQDG participates in functioning of the mechanism . -
pubmed
The conformational disorder of
PSII caused by the defect in
SQDG might be correlated with the increased susceptibility of its activity to heat - stress . -
pubmed
BACKGROUND : A prospective phase II study was performed to determine the feasibility , efficacy and safety of arterial hepatic infusion (
HAI ) using
pirarubicin combined with intravenous chemotherapy . -
pubmed
[
Brain phosphatases in
thiobarbiturate anesthesia ; histochemical study ] . -
pubmed
The peptide hormone
relaxin , which attains high circulating levels during pregnancy , has been shown to depress small - bowel motility through a
nitric oxide (
NO)-mediated mechanism . -
pubmed
In the presence of the
NO synthesis inhibitor
l - NNA ,
relaxin was ineffective . -
pubmed
Direct smooth muscle contractile responses were not influenced by
relaxin or
l - NNA . -
pubmed
Further addition of
relaxin to the bath medium did not influence EFS - induced responses , whereas
l - NNA did . -
pubmed
A significant loss of
ESP activity paralleled the decrease in
sulforaphane nitrile formation . -
pubmed
METHODS AND RESULTS : Ex vivo and in vivo sensitivity of coronary MR
thrombus imaging was tested by use of intracoronarily delivered
Gd - DTPA - labeled
fibrinogen thrombi ( n=6 ) . -
pubmed
Fibrinogen thrombi : 5 of 6 intracoronarily delivered
Gd - labeled
fibrinogen clots ( approximately 250 micromol / L Gd ) were visible on MRI and subsequently confirmed by x - ray angiography . -
pubmed
Curcumin sensitizes
tumor necrosis factor - related apoptosis - inducing ligand (
TRAIL)-induced apoptosis through reactive
oxygen species - mediated upregulation of
death receptor 5 (
DR5 ) . -
pubmed
This apoptosis induced by the combination of
curcumin and
TRAIL is not interrupted by
Bcl-2 overexpression . -
pubmed
We found that treatment with
curcumin significantly induces
death receptor 5 (
DR5 ) expression both at its mRNA and protein levels , accompanying the generation of the reactive
oxygen species ( ROS ) . -
pubmed
Not only the pretreatment with
N - acetylcystine but also the ectopic expression of
peroxiredoxin II , an antioxidative protein , dramatically inhibited the apoptosis induced by
curcumin and
TRAIL in combination , blocking the
curcumin - mediated
DR5 upregulation . -
pubmed
Taken together , the present study demonstrates that
curcumin enhances
TRAIL - induced apoptosis by ROS - mediated
DR5 upregulation . -
pubmed
Part
II : On the possible occurrence of Stereochemical Changes in Equilibrated Solutions of Reducing
Sugars introduced into the Circulation . -
pubmed
Characterization of hepatobiliary transport systems of a novel
alpha4beta1 / alpha4beta7 dual antagonist ,
TR-14035 . -
pubmed
PURPOSE : Our previous pharmacokinetic studies have demonstrated that
TR-14035 , a novel dual antagonist for
alpha4beta1 / alpha4beta7 integrin , selectively and strongly accumulated in the liver and was mainly excreted in bile as an unchanged drug . -
pubmed
OATP1B1 /
OATP - C and
OATP1B3 /
OATP8 expressed in oocytes mediated the
TR-14035 uptake with K(m ) of 7.5 and 5.3 microM , respectively . -
pubmed
OATP1B1 * 15 , a genetic variant of
OATP1B1 , exhibited a decreased transport of
TR-14035 compared with
OATP1B1 * 1a . -
pubmed
CONCLUSION : These results indicate that
OATP1B1 and
OATP1B3 are at least partly responsible for the accumulation of
TR-14035 into hepatocytes , and
Mrp2 principally mediates the biliary excretion of
TR-14035 . -
pubmed
Furthermore , genetic polymorphisms of
OATP1B1 may cause an interindividual variability in the pharmacokinetics of
TR-14035 . -
pubmed
The use of postnatal
dexamethasone was associated with shorter length , lower weight , lower weight for length and smaller head circumference ; this effect remained after correction for
GA , BW and BW -
SDS . -
pubmed
Haloperidol is an inhibitor but not substrate for
MDR1 /
P - glycoprotein . -
pubmed
The inhibitory effect of
haloperidol on other
MDR1 substrates was also investigated in terms of the optimization of
haloperidol - based pharmacotherapy . -
pubmed
The transepithelial transport of
[ ( 3 ) H ] haloperidol did not differ between the two cell lines , and
vinblastine , a typical
MDR1 substrate , had no effect on the transport , suggesting that
haloperidol is not a substrate for
MDR1 , and it is unlikely that
MDR function affects
haloperidol absorption and brain distribution , and thereby the response to
haloperidol . -
pubmed
However ,
haloperidol was found to have an inhibitory effect on the
MDR1 - mediated transport of
[ ( 3 ) H ] digoxin and
[ ( 3 ) H ] vinblastine with an IC50 value of 7.84+/-0.76 and 3.60+/-0.64 microM , respectively , suggesting that the intestinal absorption , not distribution into the brain , of
MDR1 substrate drugs could be altered by the co - administration of
haloperidol in the clinical setting , although further clinical studies are needed . -
pubmed
Besides damage to several tissues in vivo ,
AZA-1 has been shown to cause
cytotoxicity in a number of cell lines and alterations in
actin cytoskeleton and cell morphology . -
pubmed
The activation of
caspases by
AZA-1 was studied next using a fluorescent
caspase inhibitor . -
pubmed
Additionally , regarding the involvement of apoptosis / cytoskeleton signaling in
AZA-1 - induced morphological effects , inhibition of
caspases with
Z - VAD - FMK did not affect
AZA-1 - induced cytoskeletal changes , suggesting , together with the activation kinetics , that
caspases are not responsible for
AZA-1 - elicited morphological changes . -
pubmed
Modulation of
PKA ,
PKC ,
PI3 K ,
Erk ,
p38MAPK ,
glutathione and microtubules with inhibitors / activators did not inhibit
AZA-1 - induced
actin cytoskeleton rearrangement . -
pubmed
The
JNK inhibitor
SP600125 seemed to slightly diminish
AZA-1 effects , however due to the effects of the drug by itself the involvement of
JNK in
AZA-1 toxicity needs further investigation . -
pubmed
Recently , we showed that repeated activation of NKT cells by their specific glycolipid ligand ,
alpha - galactosylceramide , leads to a change in function to an immune regulatory role with
IL-10 production . -
pubmed
Five important forms of
nonallergic angioedema can be distinguished : hereditary (
HAE ) , acquired (
AAE ) ,
renin -
angiotensin -
aldosterone system ( RAAS)-blocker - induced (
RAE ) ,
pseudoallergic angioedema (
PAE ) and
idiopathic angioedema (
IAE ) . -
pubmed
Today , a plasma pool
C1 - esterase inhibitor (
C1 - INH ) concentrate is the therapy of choice in
HAE . -
pubmed
Recently , several new drugs were developed : a recombinant
C1 - INH , a
kallikrein inhibitor (
ecallantide ) and a specific
bradykinin - B2 - receptor antagonist (
icatibant ) . -
pubmed
The authors examined potential neuroprotective effects in
optic neuritis by
SRT647 and
SRT501 , two structurally and mechanistically distinct activators of
SIRT1 , an enzyme involved in cellular stress resistance and survival . -
pubmed
This neuroprotective effect was blocked by
sirtinol , a
SIRT1 inhibitor . -
pubmed
, Chen , K. H.-C. , Chen , C.-L. , and Chan , M. K. ( 2007 ) Redox potentiometry studies of particulate
methane monooxygenase : Support for a trinuclear
copper cluster active site , Angew . -
pubmed
10
Cu ( I ) ions cooperatively when
cupric ions are added to the
protein fragment in the presence of
dithiothreitol or
ascorbate . -
pubmed
The propensity for the C - terminal subdomain to bind
Cu ( I ) is consistent with the high redox potential(s ) determined for the E - cluster
copper ions in the
pMMO . -
pubmed
These properties of the E - clusters are in accordance with the function proposed for these
copper ions in the turnover cycle of the
enzyme . -
pubmed
Response to
prednisone in relation to
NR3C1 intron B polymorphisms in childhood
nephrotic syndrome . -
pubmed
In the
SRNS group , we performed a three - marker haplotype analysis of
NR3C1 in relation to the response to
prednisone , represented as time to
proteinuria resolution (
TPR ) as categorical and ordinal variable . -
pubmed
The GTA haplotype was associated with a higher
GC sensitivity , as determined by TPR , and was found to be more prevalent in early ( response & lt;or=7 days ) than late ( response & gt;7 days )
prednisone responders ( 27.7 vs. 14.5 % , hap - score = -2.22 , p = 0.05 adjusted for biopsy results ) . -
pubmed
A mutational variant of the enzyme lacking the
disulfide bond has been produced by substituting the two
cysteine residues with
alanine ( EcoAcP mutational variant
C5A / C49A , mutEcoAcP ) . -
pubmed
The wild - type protein folds according to a two - state model with a very fast rate constant ( k(F)(
H2O)=72,600 s(-1 ) ) , while
mutEcoAcP folds ca 1500 - fold slower , via the accumulation of a partially folded species . -
pubmed
The correlation also holds for
EcoAcP , provided the relative contact order value is recalculated by considering the
disulfide bridge as an alternate path for the backbone to determine the shortest sequence separation between contacting residues . -
pubmed
Sucrose gradients of serum revealed
antibody complexes in both non - responders . -
pubmed
A delta class
GST from the same species shows marginal
DDT - metabolizing activity but the activity of
agGSTe2 is approximately 350x higher than the
delta class agGST1 - 6 . -
pubmed
To investigate its catalytic mechanism and the molecular basis of its unusually high
DDT - metabolizing ability , three
agGSTe2 crystal structures including one apo form and two binary complex forms with the co - factor
glutathione (
GSH ) or the inhibitor
S - hexylglutathione (
GTX ) have been solved with a resolution up to 1.4A. -
pubmed
The binding of
GSH or
GTX does not induce significant conformational changes in the
protein . -
pubmed
The comparison with the less active
agGST1 - 6 provides the structural evidence for its high
DDT - detoxifying activity . -
pubmed
In short , this is achieved through the inclination of the upper part of
H4 helix (
H4 '' helix ) , which brings residues
Arg112 ,
Glu116 , and
Phe120 closer to the
GSH - binding site resulting in a more efficient GS(-)-stabilizing
hydrogen - bond - network and higher
DDT - binding affinity . -
pubmed
To identify genomic sequences associated with
estrogen responsiveness , the 5'-promoter region ( -44 to +118 ) of the
IGFBP-6 gene was cloned into a
chloramphenicol acetyltransferase (
CAT ) reporter vector . -
pubmed
Identification of the
estrogen - responsive element ( ERE ) [ 5 - CCTTCA CCTG-3 ] ( -9 to +1 ) in this
IGFBP-6 gene promoter region was confirmed using electromobility shift assays and deletion analysis . -
pubmed
However , the spatiotemporal expression patterns of the
AQP gene family during tubular maturation and the potential influence of genetic variation on these patterns and on
water handling remain unknown . -
pubmed
Furthermore , adult CD-1 kidneys expressed more
AQP2 in the collecting ducts , which was reflected by a significant delay in excreting a
water load . -
pubmed
These data ( 1 ) substantiate the clustering of important genes during tubular maturation and ( 2 ) demonstrate that genetic variability influences the regulation of the
AQP gene family during tubular maturation and
water handling by the mature kidney . -
pubmed
Helium decreased expression of the proinflammatory marker
CD11b and
ICAM-1 on leukocytes and attenuated the expression of the procoagulant markers
CD42b and
PSGL-1 on platelets . -
pubmed
By making use of the aggregation - induced emission feature of compound 1 and the cascade reactions among
acetylthiocholine iodide (
ATC ) ,
AChE , and compound 2 , a new fluorescence " turn - on " method is developed for
AChE assay and inhibitor - screening . -
pubmed
CTAB - extracted DNA was also purified using the Zymo ( DNA Clean & amp ; Concentrator 25 kit ) , Qtip 100 ( Qiagen Genomic - Tip 100 / G ) , and QIAEX
II Gel Extraction Kit . -
pubmed
A study of
tungsten oxide nanowires self - organized on
mica support . -
pubmed
Self - organized straight nanowires of
WO3 have been epitaxially grown on
muscovite mica in low super - saturation conditions . -
pubmed
The experimental results show that straight
WO3 nanowires are orientated preferentially parallel to two of three high symmetry crystallographic directions of
mica [ 100 ] and [ 110 ] or [ 100 ] and [ Formula : see text ] . -
pubmed
The growth mechanism has been studied as a function of different preparation conditions with special focus on the role of
potassium ions present on the
mica surface . -
pubmed
The final structural identification was performed by
MS ,
( 1 ) H - NMR and
( 13 ) C - NMR Spectra . -
pubmed
Post - resuscitation
NAC treatment also significantly attenuated the increase in cortical
caspase-3 and lipid
hydroperoxide concentrations . -
pubmed
We report that in vitro pre - treatment of PBMC with
sodium arsenite (
NaAs ) reduces
IL2 secretion and T cell proliferation induced by
PHA , but does not prevent expression of
monocyte - derived cytokines (
IL1 ,
IL6 ,
TNFalpha ) functioning as
lymphocyte - activating factors . -
pubmed
Kinetic analysis showed that
NaAs pre - treatment first inhibits , but thereafter markedly increases , induction of
IL2 and
IL2RA mRNA when T cells are stimulated with
PHA for 8 h and 72 h , respectively . -
pubmed
NaAs systematically and significantly up - regulated a set of 35 genes , including several immune and stress genes , such as
IL13 ,
granulocyte - macrophage colony stimulating factor ,
lymphotoxin alpha and
heme oxygenase-1 (
HO-1 ) . -
pubmed
Up - regulation of
HO-1 , a stress and immunosuppressive protein , was rapidly detectable , both in T cells and in PBMC treated with
NaAs . -
pubmed
Inhibition of the immunosuppressive activity of
HO-1 in PBMC however failed to prevent
NaAs - dependent inhibition of T cell proliferation induced by
PHA . -
pubmed
However , chronic
THC increased T lymphocyte
CXCR4 expression on both
CD4 + and
CD8 + T lymphocytes compared to control . -
pubmed
The use of
dexmedetomidine , an
alpha - adrenergic receptor agonist , for sedation may reduce intensive care unit length of stay when compared with use of
benzodiazepines . -
pubmed
Using comprehensive gene expression analyses , we have identified
selenoprotein P(
SeP ) as a novel hepatokine that elevates plasma
glucose levels by inducing
insulin resistance . -
pubmed
BACKGROUND : This is the first study to compare plasma and cerebrospinal fluid (
CSF ) pharmacokinetics of intravenous (
IV ) , oral ( PO ) , or rectal ( PR ) formulations of
acetaminophen . -
pubmed
Acetaminophen plasma and
CSF levels were obtained at T0 , 0.25 , 0.5 , 0.75 , 1 , 2 , 3 , 4 , and 6 hours . -
pubmed
RESULTS :
IV acetaminophen showed earlier and higher plasma and
CSF levels compared with PO or PR administration . -
pubmed
Inhibition of forebrain
mu - opioid receptor signaling by low concentrations of
rimonabant does not require
cannabinoid receptors and directly involves
mu - opioid receptors . -
pubmed
Increasing number of publications shows that
cannabinoid receptor 1 (
CB ( 1 ) ) specific compounds might act in a CB(1 ) independent manner , including
rimonabant , a potent
CB ( 1 ) receptor antagonist . -
pubmed
rimonabant treatment prior to that of NE treatment caused further attenuation on the maximal stimulation of
Tyr - d - Ala - Gly -( NMe ) Phe - Gly - ol (
DAMGO ) , which is a highly specific
MOR agonist . -
pubmed
In radioligand competition binding assays we highlighted that
rimonabant fails to displace effectively
[ ( 3 ) H ] DAMGO from
MOR in low concentrations and is highly unspecific on the receptor at high concentrations in
CB ( 1 ) knockout forebrain and in their wild - type controls . -
pubmed
Surprisingly , docking computational studies showed a favorable binding position of
rimonabant to the inactive conformational state of
MOR , indicating that
rimonabant might behave as an antagonist at
MOR . -
pubmed
Estrogenic compounds were detected ( average of combined solids and liquid fractions ) at average concentrations of : 1.42 ng / L for
estrone , 0.69 ng / L for
17beta estradiol , 3.83 ng / L for
estriol (
E3 ) , 0.56 ng / L for
17alpha - ethynylestradiol , 64.2 ng / L for
bisphenol A , 7.51 ng / L for
4 - nonylphenol and 5.93 ng / L for
4 - tert - octylphenol . -
pubmed
Recombinant His - tagged
PhpA requires
Mn ( 2 + ) or
Co ( 2 + ) for
phosphatase activity , and shows strict specificity for phosphorylated
tyrosine residues . -
pubmed
The k(m ) values of
PhpA for
p - nitrophenyl phosphate (
pNPP ) and
phosphotyrosine peptide , RRLIEDAEpYAARG , were 803 and 139 microM , respectively . -
pubmed
The
phosphatase activity of
PhpA was inhibited by
sodium orthovanadate with a k(i ) of 33 microM. -
pubmed
A
phpA mutant exhibited an elevated level of
tyrosine phosphorylation of a 79 kDa protein and
cytoplasmic tyrosine kinase ,
BtkA. -
pubmed
The presence of intracellular
dopamine can further enhance the relative abundance of
aSynL transcripts through alternative polyadenylation site selection . -
pubmed
High sensitivity of diffusion - weighted MR imaging for the detection of
liver metastases from
neuroendocrine tumors : comparison with
T2 - weighted and dynamic
gadolinium - enhanced MR imaging . -
pubmed
PURPOSE : To compare the sensitivity and specificity of diffusion - weighted ( DW ) magnetic resonance ( MR ) imaging for identifying liver
metastases from
neuroendocrine tumor (
NET ) to those of
T2 - weighted fast spin - echo ( FSE ) and three - dimensional dynamic
gadolinium - enhanced MR imaging , with surgical and histopathologic findings as the reference standard . -
pubmed
DW MR was more sensitive ( observer 1 : sensitivity , 71.6 % [ 116 of 162 ] , 95 % confidence interval [ CI ] : 64.2 % , 78.0 % ; observer 2 : sensitivity , 71.0 % [ 115 of 162 ] , 95 % CI : 63.6 % , 77.4 % ) than
T2 - weighted FSE ( observer 1 : sensitivity , 55.6 % [ 90 of 162 ] , 95 % CI : 47.9 % , 63.0 % ; observer 2 : sensitivity , 55.6 % [ 90 of 162 ] , 95 % CI : 47.9 % , 63.0 % ) and dynamic
gadolinium - enhanced MR ( observer 1 : sensitivity , 47.5 % [ 77 of 162 ] , 95 % CI : 34.0 % , 55.2 % ; observer 2 : sensitivity , 48.1 % [ 78 of 162 ] , 95 % CI : 40.6 % , 55.8 % ) ( P & lt ; .001 for both , McNemar test ) . -
pubmed
The specificity of these sequences ranged from 88.9 % to 100 % ( DW MR vs
T2 - weighted FSE MR : P & gt ; .99 , DW MR vs dynamic
gadolinium - enhanced MR : P = .61 , and
T2 - weighted FSE MR vs dynamic
gadolinium - enhanced MR : P = .61 , McNemar test ) . -
pubmed
CONCLUSION : DW MR imaging was more sensitive for the detection and characterization of
liver metastases from
NETs than
T2 - weighted FSE and dynamic
gadolinium - enhanced MR imaging and should be systematically performed . -
pubmed
While the down - regulated four genes ,
MAPK4 ,
FASN ,
INSIG1 and
SCD , were mainly linked to the endoplasmic reticulum and
fatty acid biosynthesis , these two cell processes that are associated with drug sensitivity by increasing evidences . -
pubmed
RESULTS : Overexpression of
TGF - beta1 and
phospho -
Smad2 , and reduced expression of membrane
beta - catenin , were closely associated with lung
metastasis in
ACC . -
pubmed
CONCLUSION :
TGF - beta1 ,
phospho -
Smad2 and
beta - catenin were significantly correlated with
ACC metastasis . -
pubmed
Changes of
PCr ,
ATP , inorganic
phosphate , and
phosphomonoesters were measured by means of
( 31 ) Phosphorous - magnetic resonance spectroscopy during and
post - exercise . -
pubmed
RESULTS : Average PCr ( percentage of [
PCr ] at initial
rest ( % [
PCr]i ) ) at the end of the exercise ( adults 17 + - 12 % [
PCr]i , children 38 + - 17 % [
PCr]i , P & lt ; 0.01 ) and recovery periods ( adults 37 + - 14 % [
PCr]i , children 57 + - 17 % [
PCr]i , P & lt ; 0.01 ) was significantly lower in adults compared to children , induced by a stronger
PCr decrease during the first exercise interval ( adults -73 + - 10 % [
PCr]i , children -55 + - 15 % [
PCr]i , P & lt ; 0.01 ) . -
pubmed
Glucose tolerance ,
insulin resistance ,
insulin secretory capacity , anthropometric measurements , lipid profiles , nutrient intakes and pregnancy outcomes were evaluated at 24 - 28 weeks of gestation . -
pubmed
Characterization of
CM572 , a Selective Irreversible Partial Agonist of the
Sigma-2 Receptor with Antitumor Activity . -
pubmed
Here , we characterize
CM572 [
3 -( 4 -( 4 -( 4 - fluorophenyl ) piperazin-1 - yl ) butyl )- 6 - isothiocyanatobenzo [ d ] oxazol-2 ( 3H )- one ] ( sigma-1 Ki & gt;= 10 microM , sigma-2 Ki = 14.6 + - 6.9 nM ) , a novel
isothiocyanate derivative of the putative
sigma-2 antagonist ,
SN79 [
6 - acetyl-3 -( 4 -( 4 -( 4 - fluorophenyl ) piperazin-1 - yl ) butyl ) benzo [ d ] oxazol-2 ( 3H )- one ] . -
pubmed
CM572 bound irreversibly to
sigma-2 receptors by virtue of the
isothiocyanate moiety but not to sigma-1 . -
pubmed
Low concentrations of
CM572 that were minimally effective were able to attenuate significantly the
calcium signal and cell
death induced by the
sigma-2 agonist
CB-64D [
( + ) - 1R,5R -( E )- 8 - benzylidene-5 -( 3 - hydroxyphenyl )- 2 - methylmorphan-7 - one ] . -
pubmed
Taken together , these data show that
CM572 is a selective , irreversible
sigma-2 receptor partial agonist . -
pubmed
at 30mM
NaCl , actual quantum efficiency of
photosystem II values were 63 - 96 % of controls ) . -
pubmed
In the present paper we wanted to investigate lungs exposed to 1 h of warm
ischemia without
heparin followed by
flush - perfusion and cold storage compared to lungs harvested from heart beating donors (
HBD ) using standard harvesting technique . -
pubmed
An Overview of
Insulin Pumps and
Glucose Sensors for the Generalist . -
pubmed
Applying differential microscale stresses and
strains showed that
hyaluronic acid ( HA ) gels display semi - flexible polymer behavior , while
laminin - rich ECM hydrogels display flexible polymer behavior . -
pubmed
In this work , we developed a novel layered
molybdenum disulfide (
MoS2 ) nanosheet
peroxidase mimetic - based fluorescent catalytic biosensor for the sensitive and selective detection of
Fe ( 2 + ) . -
pubmed
To elucidate underlying mechanisms , we assessed the arrhythmogenic SR -
Ca ( 2 + ) leak at the time of LVAD implantation (
HF - Im ) and heart transplantation (
HF - Tx ) and evaluated the effects of
CaMKII - inhibition . -
pubmed
In sum ,
CaMKII inhibition yielded a clear tendency toward a reduction of the
SR -
Ca ( 2 + ) leak ( n cells / patients = 76/6 vs. 108/6 , P = 0.08 ) . -
pubmed
In
HF - Tx , we detected an even higher CaSpF of 1.00 + - 0.10 100 mum(-1 ) x s(-1 ) and a higher
SR -
Ca ( 2 + ) leak compared with
HF - Im ( increase by 81 + - 33 % , n cells / patients = 156/7 vs. 130/7 , P & lt ; 0.05 ) , which fits to the further decreased LV function . -
pubmed
These data show that the
SR -
Ca ( 2 + ) leak correlates with the development of LV function after LVAD implantation and may represent an important pathomechanism . -
pubmed
The fact that
CaMKII inhibition reduces the SR -
Ca ( 2 + ) leak in
HF - Tx suggests that
CaMKII inhibition may be a promising option to beneficially influence clinical course after LVAD implantation . -
pubmed
Children with severe forms of
malaria had significantly higher
IgG to merozoite
antigens ( all p & lt ; 0.05 ) , but not to
VAR2CSA ( all p & gt ; 0.05 ) when compared to the healthy children . -
pubmed
An organic
imidazolium salt of
terephthalic acid has been utilized as a linker precursor for the synthesis of an
aluminum - based metal organic framework (
MOF ) with
MIL-53(ht ) structure . -
pubmed
Investigating Mutations to Reduce
Huntingtin Aggregation by Increasing
Htt - N - Terminal Stability and Weakening Interactions with
PolyQ Domain . -
pubmed
Huntington ' s disease is a fatal
autosomal genetic disorder characterized by an expanded
glutamine - coding CAG repeat sequence in the
huntingtin (
Htt ) exon 1 gene . -
pubmed
Competing models for the misfolding and aggregation phenomena have suggested the role of the
Htt - N - terminal region and the CAG
trinucleotide repeats (
polyQ domain ) in affecting aggregation propensities and misfolding . -
pubmed
We investigate the mutation landscape of the
Htt - N - terminal region and explore
amino acid residue mutations that affect its structural stability and hydrophobic interactions with the
polyQ domain . -
pubmed
The
serotonin transporter (
SERT ) and other
monoamine transporters operate in either a forward transport mode where the transporter undergoes a full transport cycle or an exchange mode where the transporter seesaws through half - cycles . -
pubmed
This prediction was verified by tryptic digestion of
SERT - expressing membranes : in the absence of
Na + , the N terminus was rapidly digested . -
pubmed
However ,
amphetamine - induced efflux by
SERT - DeltaN32 or
SERT - DeltaN42 ( but not by
SERT - DeltaN22 ) was markedly diminished . -
pubmed
The findings are consistent with a model where the N terminus acts as a lever to support
amphetamine - induced efflux by
SERT . -
pubmed
This potentially suggests involvement of
CAT8 in
xylose metabolism . -
pubmed
Constructed
cat8Delta strain isolated from wild
strain showed diminished growth on
glycerol ,
ethanol and
xylose as well as diminished respiration on the last substrate . -
pubmed
CAT8 deletant was characterized by activated transcription of genes
XYL3 ,
DAS1 and
RPE1 and slight increase in the activity of several enzymes involved in
xylose metabolism and alcoholic fermentation . -
pubmed
Ethanol production from
xylose in
cat8Delta mutants in the background of wild - type
strain and the best available
ethanol producer from
xylose increased for 50 and 30 % , respectively . -
pubmed
The maximal titer of
ethanol during
xylose fermentation was 12.5 g
ethanol / L at 45 C. Deletion of
CAT8 did not change
ethanol production from
glucose . -
pubmed
Available data suggest on specific role of
Cat8 in
xylose alcoholic fermentation . -
pubmed
Pingyangmycin Pretreatment Influences the Biological Behavior of
Ocular Venous Malformation and Relates with
Galectin-3 Expression . -
pubmed
We conducted this study to investigate the effect of
pingyangmycin pretreatment on the
Gal-3 expressions and biological behavior of
ocular venous malformation . -
pubmed
RESULTS :
Gal-3 protein was expressed in 35 ( 52 % ) of 67 samples in the nonpingyangmycin group and in 19 ( 28 % ) of 69 samples in the
pingyangmycin group ( P & lt ; 0.05 ) . -
pubmed
Gal-3 mRNA expression was detected in 39 ( 58 % ) of 67 samples in the nonpingyangmycin group and 22 ( 32 % ) of 69 samples in the
pingyangmycin group ( P & lt ; 0.05 ) . -
pubmed
The higher
Gal-3 expressions were detected in samples with deeper invasiveness than those with superficial invasiveness before ( chi2 = 12.720 and 13.369 , respectively , both P & lt ; 0.05 ) and after
pingyangmycin treatment ( chi2 = 8.429 and 4.590 , respectively , both P & lt ; 0.05 ) . -
pubmed
Pingyangmycin treatment led to a significant difference in
Gal-3 expressions at both protein and mRNA levels ( chi2 = 8.664 and 9.524 , respectively , both P & lt ; 0.05 ) . -
pubmed
CONCLUSIONS :
Gal-3 expression may be involved in the development and invasiveness of
ocular venous malformation , and
pingyangmycin can inhibit
Gal-3 expression , indicating a role of
pingyangmycin treatment before the removal of
ocular venous malformation . -
pubmed
The
beta - galactoside binding protein
galectin-7 (
gal-7 ) is constitutively expressed at abnormally high levels in the outside milieu and intracellular compartments of many types of
epithelial cancer cells , most notably in aggressive forms of
ovarian and breast cancer . -
pubmed
The
ADP purinergic receptor P2Y12 is mainly expressed by platelets with important clinical significance as a target for several clinically approved antithrombotic agents . -
pubmed
Clopidogrel treated
TSSC6 KO arterioles showed smaller and less stable
thrombi with increased tendency to embolise in vivo . -
pubmed
PURPOSE : Epidemiological information on the association between
sugar - sweetened beverage (
SSB ) consumption and the risk for
hypertension (
HTN ) in Koreans is very limited . -
pubmed
Current therapy relies on administration of
acetylcholinesterase inhibitors ( AChEIs ) -
donepezil ,
rivastigmine ,
galantamine , and
N - methyl - d - aspartate receptor antagonist
memantine . -
pubmed
In this review article , we made comprehensive literature survey emphasising on
donepezil template which was structurally converted into plethora of MTLDs preserving anti -
cholinesterase effect and , at the same time , escalating the anti - oxidant potential , which was reported as a crucial role in the pathogenesis of the
Alzheimer ' s disease . -
pubmed
Pyruvate carboxylase (
PC ) catalyzes the conversion of
pyruvate to
oxaloacetate (
OAA ) , an important metabolic reaction in a wide range of organisms . -
pubmed
AIBP decreases
atherogenesis by augmenting
cholesterol efflux . -
pubmed
We found that
ISL administration reduced
serum amylase and
lipase levels and alleviated the histopathological manifestations of pancreatic tissue in a dose - dependent manner . -
pubmed
Meanwhile ,
ISL decreased the oxidative stress
injury and increased the protein expression of the
Nrf2 /
HO-1 pathway . -
pubmed
Methods : In this randomized controlled trial , women with
ovarian or endometrial cancer [ age : & gt;=19 y ;
body mass index ( kg / m2 ): & gt;=18.5 ] were randomly assigned to a KD ( 70:25:5 energy from fat , protein , and
carbohydrate ) or the American Cancer Society diet (
ACS ; high - fiber , low - fat ) . -
pubmed
Body composition ( DXA ) and fasting serum
insulin ,
IGF - I , and
beta - hydroxybutyrate were obtained at baseline and at 12 wk ; urinary
ketones were also measured throughout the intervention . -
pubmed
The animals were treated with 20 mg / kg / day
losartan , an antagonist of
AT1 receptor , or
captopril , an
angiotensin - converting enzyme inhibitor . -
pubmed
It was shown that the mechanism of cell
death caused by BRET - induced
phototoxicity of mimiSOG in the presence of
furimazine depends on the intracellular localization of the NanoLuc -
miniSOG fusion protein : BRET - mediated activation of
miniSOG in mitochondrial localization causes apoptosis , while the membrane localization of PS causes
necrosis of
cancer cells . -
pubmed
Fasting blood samples were obtained to analyze the serum concentration of bone turnover markers including
osteocalcin (
OC ) ,
beta - isomerized type I collagen amino - terminal peptide (
beta - CTX ) ,
alkaline phosphatase (
ALP ) ,
type I procollagen amino - terminal peptides (
PINP ) ,
calcium , and
phosphorus . -
pubmed
CONCLUSIONS : These findings indicate the action of at least two different
carotenoid cleavage dioxygenases (
CCD2 and tentatively
CCD4 ) during biosynthesis of this new
bis - apocarotenoid aglycon (
norcrocetin ) and the existence of even higher
glycosylated crocin derivatives at trace level . -
pubmed
The
mevalonate pathway provides
sterols for membrane structure and
nonsterol intermediates for the
post - translational modification and membrane anchorage of growth - related proteins , including the
Ras ,
Rac , and
Rho GTPase family . -
pubmed
Mevalonate - derived products are also essential for the
Hedgehog pathway ,
steroid hormone signaling , and the nuclear localization of
Yes - associated protein and transcriptional co - activator with PDZ - binding motif , all of which playing roles in
tumorigenesis and
cancer stem cell function . -
pubmed
The
phosphatidylinositol-4,5 - bisphosphate 3 - kinase -
AKT -
mammalian target of rapamycin complex 1 pathway ,
p53 with gain - of - function mutation , and oncoprotein
MYC upregulate the
mevalonate pathway , whereas
adenosine monophosphate - activated protein kinase and
tumor suppressor protein RB are the downregulators . -
pubmed
Sterol regulatory element binding protein 2 mediates the
sterol - controlled transcriptional downregulation of
HMGCR . -
pubmed
UbiA prenyltransferase domain - containing protein-1 regulates the ubiquitination and proteasome - mediated degradation of
HMGCR , which is accelerated by
24 , 25 - dihydrolanosterol and the
diterpene geranylgeraniol . -
pubmed
Statins , competitive inhibitors of
HMGCR , deplete cells of
mevalonate - derived intermediates and consequently inhibit cell proliferation and induce apoptosis . -
pubmed
Tumor HMGCR is resistant to the
sterol - mediated transcriptional control ; consequently ,
HMGCR is upregulated in
cancers derived from adrenal gland , blood and lymph , brain , breast , colon , connective tissue , embryo , esophagus , liver , lung , ovary , pancreas , prostate , skin , and stomach . -
pubmed
Nevertheless ,
tumor HMGCR remains sensitive to
isoprenoid - mediated degradation . -
pubmed
The contents of
PFAAs and the FDOM in the water samples were measured by a high - performance liquid chromatograph - mass spectrometer ( HPLC -
MS ) and by a fluorescence spectrophotometer , respectively . -
pubmed
Compared to
LH /
FSH - secreting
tumors ,
PRL - secreting
tumors showed statistically significant decrease in the levels of
N - acetylaspartate (
NAA ) ,
myo - inositol ( mI ) ,
scyllo - inositol (
sI ) ,
glycine ,
taurine ,
phosphoethanolamine (
PE ) and increase in the levels of
glutamine . -
pubmed
When compared with
LH /
FSH - secreting
tumors ,
ACTH - secreting
tumors showed statistically significant decrease in the levels of sI ,
glycine , PE and increase in the levels of
aspartate . -
pubmed
Although lipid extracts of PAs showed the presence of many common lipid molecules , only
glycerophosphoethanolamine (
GPE ) showed statistically significant decrease in
PRL ,
ACTH and non - functional subtypes when compared to
LH /
FSH - secreting
tumors . -
pubmed
After EA treatment ,
SAS and
SDS scores were decreased in the acupuncture group ( P=0.007 and P=0.027 , respectively ) and were lower than those of the control group ( P=0.003 and P=0.004 , respectively ) . -
pubmed
Hydroxymethylene diphosphonate for imaging
transthyretin cardiac amyloidosis . -
pubmed
RESULTS :
T1 increased crude proteins , fat ,
Ca ,
Fe ,
vitamin C , total phenolic content , antioxidant capacity by
DPPH and by ORAC . -
pubmed
On the other hand ,
T1 decreased reducing
sugars , K and
Cu content . -
pubmed
We measured their
alpha - gal A activity and plasma/ serum
globotriaosylsphingosine (
lyso - Gb3 ) accumulation and performed
electron microscopy of skin biopsies . -
pubmed
Multiple linear regression was carried out to construct an Immunity model ( adjusted
R2 = 75.8 % ) comprising Macrophage chemotaxis and phagocytosis and Lymphoproliferation capacity , a Redox model ( adjusted
R2 = 84.4 % ) involving Reduced
Glutathione and
Malondialdehyde concentrations and
Glutathione Peroxidase activity and a Behavioural model ( adjusted
R2 = 79.8 % ) comprising Internal Locomotion and Time spent in open arms indices . -
pubmed
UCP2 Deficiency Increases Colon Tumorigenesis by Promoting Lipid Synthesis and Depleting
NADPH for Antioxidant Defenses . -
pubmed
Mechanistically ,
UCP2 loss increases levels of
oxidized glutathione and proteins in
tumors . -
pubmed
UCP2 deficiency alters glycolytic pathways while promoting phospholipid synthesis , thereby limiting the availability of
NADPH for buffering oxidative stress . -
pubmed
Metformin attenuates
PD - L1 expression through activating Hippo signaling pathway in
colorectal cancer cells . -
pubmed
Here , we showed that
metformin decreases
PD - L1 and
YAP1 expression in vitro and in vivo . -
pubmed
After silencing or inhibiting
YAP1 expression by
Verteporfin (
VP ) , the inhibitor of
YAP1 , the expression of
PD - L1 were decreased in protein level in
CRC cells . -
pubmed
Compared with the control group ,
PD - L1 and
YAP1 expressions in
tumor tissues , detected by immunohistochemistry , were reduced in the group of
metformin treatment . -
pubmed
In order to develop an affinity HPLC method for screening direct
thrombin inhibitors from Traditional Chinese Medicine ( TCM ) ,
thrombin was immobilized on the
glutaraldehyde - modified
amino silica gel and was used as
thrombin stationary phase . -
pubmed
Thirty - two crowns were studied and divided into four groups ( n = 8) , according to the ceramic material ( feldspathic glass - ceramic ( F ) and
zirconia reinforced
lithium silicate glass - ceramic (
ZLS ) ) and to their technological obtaining processes ( milling ( M ) and heat - pressing ( P ) ) . -
pubmed
AIMS : To conduct a systematic review and meta - analysis to assess the efficacy , safety and tolerability of
sodium - glucose co - transporter-2 inhibitors vs placebo as add - on therapy after
metformin and
dipeptidyl peptidase-4 inhibitor dual therapy in
type 2 diabetes . -
pubmed
A search was conducted in the PubMed , www.clinicaltrials.gov and Cochrane Central Register of Controlled Trials databases for relevant randomized controlled trials up until 14 August 2020 that compared
sodium - glucose co - transporter-2 inhibitors vs placebo as add - on therapy after
metformin and
dipeptidyl peptidase-4 inhibitor therapy . -
pubmed
Compared with placebo ,
sodium - glucose co - transporter-2 inhibitor treatment , as add - on to
metformin and
dipeptidyl peptidase-4 inhibitor therapy , was associated with a significant reduction in HbA1c level [ mean difference -8 mmol / mol , 95 % CI -10 , -6 ( -0.7 % , 95 % CI -0.9 , -0.6 ) ; P < 0.00001 ] , in fasting plasma
glucose level [ mean difference -1.70 mmol / l , 95 % CI -1.91 , -1.49 ; P < 0.00001 ] , in weight ( mean difference -1.76 kg , 95 % CI -2.04 , -1.48 ; P < 0.00001 ) and in blood pressure (
systolic blood pressure : mean difference -3.6 mmHg , 95 % CI -4.8 , -2.4 ; P<0.00001 ; diastolic blood pressure : mean difference -1.5 mmHg ; 95 % CI -2.4 , -0.6 ; P=0.002 ) . -
pubmed
Maternal
Prenatal Depression in Pregnancies With Female and Male Fetuses and Developmental Associations With
C - reactive Protein and
Cortisol . -
pubmed
Maternal prenatal
C - reactive protein and hair
cortisol and
cortisone levels were determined . -
pubmed
Maternal
cortisol levels were significantly associated with
depression only in pregnancies with female fetuses and with decreased newborn
P50 inhibition only in female newborns . -
pubmed
In pregnancies with male fetuses compared with pregnancies with female fetuses ,
cortisol was more robustly metabolized to
cortisone , which does not activate
cortisol receptors . -
pubmed
The successful use of
PARP1 inhibitors like
olaparib (
Loparza ) in the treatment of
BRCA1 / 2 - deficient breast cancer has provided clinical proof of concept for applying personalized medicine based on synthetic lethality to the treatment of
cancer . -
pubmed
Unfortunately , all marketed
PARP1 inhibitors act by competing with the cofactor
NAD+ and resistance is already developing to this anti -
cancer mechanism . -
pubmed
identified
5F02 as an allosteric
PARP inhibitor that acts by preventing the enzymatic activation of
PARP1 by histone
H4 . -
pubmed
NMDAR antibody
encephalitis overlapping with
CLIPPERS syndrome in a
psoriasis patient on
adalimumab . -
pubmed
Fractions eluting at different
phosphate concentrations were analysed for the presence of
EBNA by means of an anti -
EBNA - specific
125I - labelled
IgG absorption assay . -
pubmed
Decreased fidelity of
DNA polymerase activity during
N-2 - fluorenylacetamide hepatocarcinogenesis . -
pubmed
The fidelity of polymerization of these
enzymes was compared by determining the incorporation of noncomplementary
deoxyribonucleoside triphosphates ( misincorporation ) on a poly(dA - dT).
poly ( dA - dT ) template , with
MnCl2 and
MgCl2 as divalent cations . -
pubmed
The results indicate that
alcohol dehydrogenase and
aldehyde dehydrogenase do not share a common pool of
NAD , and that
NADH formed during
acetaldehyde oxidation is utilized for reductions in the cytosol to a smaller extent than the
NADH formed in the
alcohol dehydrogenase reaction . -
pubmed
Chemical modifications of
spinach leaf nitrate reductase , and its 28,000 M(r ) fragment with
phenylglyoxal ,
2,3 - butanedione and
pyridoxal phosphate reduce the catalytic activity of the enzyme . -
pubmed
NADH protects the inactivation of
NADH - ferricyanide reductase activity of the 28,000 M(r ) fragment of
nitrate reductase . -
pubmed
These data suggest that
nitrate reductase contains active sites of
arginine and
lysine residues that are involved in the
NADH binding site of the enzyme . -
pubmed
The two agents evaluated were
polylysine 111In -
antimyosin ( 0.023 mg Fab modified with a 3.3 kd polymer of
polylysine and labeled with
111In ) and
99mTc -
antimyosin ( 0.5 mg
Fab ' antimyosin labeled using the RP-1 ligand technique ) . -
pubmed
Chronic blockade of the endogenous opioids with the long - acting opioid antagonist
naltrexone could result in increased
gonadotropin secretion and ovulation induction in these cases . -
pubmed
The new oral third generation
cephalosporins are effective in
infections due to
beta - lactamase - producing Haemophilus
strains . -
pubmed
Cell extracts of
3 - hydroxybenzoate - grown cells exhibited
3 - hydroxybenzoyl - CoA synthetase activity of 190 nmol min-1 mg protein-1 as well as
benzoyl - CoA synthetase activity of 86 nmol min-1 mg protein-1 . -
pubmed
Pertussis toxin pre - treatment only partially blocked presynaptic inhibition caused by
noradrenaline but abolished the reduction of EPSP amplitude by
somatostatin . -
pubmed
The presynaptic inhibitory action of
noradrenaline results from activation of
alpha 2 - adrenoceptors at nerve terminals but the mechanism(s ) by which these presynaptic receptors act can not be explained adequately by either activation of a
potassium conductance and/or inhibition of a
calcium conductance . -
pubmed
Plasma membrane
sphingomyelin and the regulation of
HMG - CoA reductase activity and
cholesterol biosynthesis in cell cultures . -
pubmed
We have examined the mechanism of the inhibition of
cholesterol synthesis in cells treated with exogenous
sphingomyelinase . -
pubmed
Free
fatty acid synthesis or general cellular protein synthesis was unaffected by the addition of
sphingomyelinase . -
pubmed
,
ceramide and
phosphocholine , had no effect on
reductase activity . -
pubmed
Sphingosine , a further product of
ceramide metabolism , caused a stimulation of
reductase activity . -
pubmed
Examination of the incorporation of
[ 3H ] acetate into the nonsaponifiable lipid fractions in the presence of
sphingomyelinase showed no changes in the percent distribution of radioactivity in the
post -
mevalonate intermediates of the
cholesterol biosynthetic pathway , but there was increased radioactivity associated with the polar
sterol fraction . -
pubmed
Pretreatment of cells with
ketoconazole , a known inhibitor of
oxysterol formation , prevented the inhibition of
reductase activity by
sphingomyelinase and decreased the incorporation of
[ 3H ] acetate in the polar
sterol fraction . -
pubmed
Ketoconazole had no effect on exogenous
sphingomyelinase activity in vitro in the presence or absence of cells . -
pubmed
Endogenous
sphingomyelinase activity was also unaffected by
ketoconazole . -
pubmed
,
chlorpromazine ,
desipramine , and
N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalene sulfonamide ( W-7 ) , to the culture medium caused a dose - dependent stimulation of
reductase activity . -
pubmed
Treatment of cells with small unilamellar vesicles of
dioleyl phosphatidylcholine or
high density lipoprotein3 resulted in increased efflux of
cholesterol and stimulation of
reductase activity . -
pubmed
Under similar conditions , the inhibitory effect of exogenous
sphingomyelinase on
reductase activity was prevented by incubation with small unilamellar vesicles of
phosphatidylcholine or
high density lipoprotein . -
pubmed
Cathepsin H splits off only one N - terminal
amino acid from each tuftsin and
enkephalin , which , according to the literature , led to inactivation of peptides . -
pubmed
The enzyme acts on the oxidized B chain of
insulin as an
aminoendopeptidase : it splits off the N - terminal
phenylalanine and the centrally located bond(s ) . -
pubmed
Km and Vmax for the
cathepsin H catalyzed hydrolysis of
LeuNA ,
ArgNA LysNA and
BANA were determined . -
pubmed
Based on the data obtained and the previously reported results on conversion of
kallidin into
bradykinin , the specificity of
cathepsin H and its possible biological functions were discussed . -
pubmed
Cloned T cells or cells from lymphoid organs were stimulated with antigen , concanavalin A , or
phorbol myristate acetate and
ionomycin in microwells coated with antibodies specific for
IFN - gamma . -
pubmed
Similarly , using
TNF - specific antibody reagents , TNF - secreting cells were detected and quantitated in cell populations obtained from
normal lymphoid tissues , bone marrow and peripheral blood , following activation with
phorbol myristate acetate and
ionomycin . -
pubmed
Effect of
cetyltrimethylammonium on
ATP hydrolysis and proton translocation in the
F0 - F1 H +- ATP synthase of mitochondria . -
pubmed
The action of
opiate receptor agonists :
( D - Ala2 )- methionine enkephalinamide (
D - MEA ) ,
morphine ,
heroin ,
etorphine , and antagonists :
naloxone and
diprenorphine on
audiogenic seizures was tested during
ethanol abstinence . -
pubmed
The first mechanism is
renin independent , requires antecedent
sodium retention and appears related to abnormal membrane transport of
calcium . -
pubmed
This vasoconstriction is identified by low plasma
renin and ionized
calcium and is correctable by
sodium depletion or
calcium channel or alpha blockade . -
pubmed
The second vasoconstrictor mechanism is
renin mediated and involves an increase in cytosolic
calcium . -
pubmed
This mechanism is quantifiable by the
plasma renin level or the
hypotensive response to an antirenin - system drug ( converting enzyme inhibitor , beta blocker or
saralasin ) . -
pubmed
In this study , the fate of the polar AFNs during early development of Tubifex embryos has been followed using rhodamine -
phalloidin as a specific stain for
F - actin . -
pubmed
The
aromatic azido group in anti -
cancer drug design : application in the development of novel lipophilic
dihydrofolate reductase inhibitors . -
pubmed
Two properties of the
aromatic azido group have been exploited in the design of a novel
dihydrofolate reductase inhibitor -- lipophilicity and biotransformability . -
pubmed
This study examines the effects of
N alpha - acetyl - beta - endorphin on tissue
ODC activity to determine whether
post - translational acetylation of
beta - endorphin at the N alpha - terminus affects its capability to alter
ODC activity and , consequently , tissue development . -
pubmed
Naloxone inhibited the stimulatory actions of N alpha - acetyl - beta - endorphin on brain
ODC completely , indicating the involvement of
opioid receptors in that process . -
pubmed
On the other hand , the increases in liver
ODC were not prevented by
naloxone , suggesting that these effects are not mediated through opioid - sensitive structures. -
pubmed
In addition ,
S -(+)-enantiomers of
aporphines and dopaminergic antagonists are equally potent as inhibitors of this
enzyme , as compared to the corresponding
R -(-)-enantiomers and other
aporphine agonists . -
pubmed
These results suggest that the inhibitory effects of
apomorphine and its analogs against this
enzyme are not mediated by their stimulation of
dopamine autoreceptors . -
pubmed
Since
dihydropteridine reductase is required in vivo for the hydroxylation of
tyrosine , the inhibition of this
enzyme by
apomorphine may represent one of several mechanisms by which
apomorphine inhibits
catecholamine synthesis . -
pubmed
We now report that pure
CRBP delivers
retinol to the specific nuclear binding sites without itself remaining bound . -
pubmed
CRBP is also capable of delivering
retinol specifically to isolated chromatin with no apparent loss of binding sites , as compared to whole nuclei . -
pubmed
CRBP again does not remain bound after transferring
retinol to the chromatin binding sites . -
pubmed
When isolated nuclei are incubated with
[ 3H ] retinol -
CRBP , sectioned , and autoradiographed , specifically bound
retinol is found distributed throughout the nuclei . -
pubmed
Thus ,
CRBP delivers
retinol to the interior of the nucleus , to specific binding sites which are primarily , if not solely , on the chromatin . -
pubmed
This
laminin binding is
calcium - dependent and can be competed by heparin . -
pubmed
Twenty - two
thrombovascular accidents were seen ( 16 % , 13 during
acetylsalicylic acid and 9 during placebo ,
NS ) , including 17
fistula thromboses . -
pubmed
The synergistic effect of the combination of
VIP with
noradrenaline ( NA ) was antagonized by pretreatment with
prazosin or
phentolamine but not by pretreatment with
yohimbine or
propranolol . -
pubmed
Distal accentuation of conduction slowing in
polyneuropathy associated with antibodies to
myelin - associated glycoprotein and
sulphated glucuronyl paragloboside . -
pubmed
With the use of the antioxidant
alpha - tocopherol with essential 12 hours after the development of
peritonitis no significant changes occurred in the
LPO values . -
pubmed
Ventilator pressures were limited to & lt ; or = 39/9 cm
H2O in the CMV -
HI group . -
pubmed
Remission maintenance therapy with
histamine and
interleukin-2 in
acute myelogenous leukaemia . -
pubmed
Histamine , of concentrations within the micromolar range , abrogated the monocyte - induced inhibition of NK - cells ; thereby ,
histamine and
IL-2 synergistically induced NK - cell - mediated killing of
AML blasts . -
pubmed
The effect of
histamine was apparently mediated by
H2 - type histamine receptors (
H2R ) , since the
H2R antagonist
ranitidine completely blocked the response . -
pubmed
Toxicity was moderate and include local reactions to
IL-2 at the site of injection and short - lasting
flush ,
hypotension , and
headache to
histamine . -
pubmed
The addition of
histamine to treatment with
IL-2 significantly enhanced the accumulation of
CD25 + T cells in peripheral blood as compared to treatment with
IL-2 alone ( P< ; 0.003 ) . -
pubmed
The N - terminal segment which contains the first three
disulfide bridges is similar to
epidermal growth factor . -
pubmed
An inactivating
Asn -
Ser mutation in this region showed the same 3D structure indicating that these three residues , and possibly an additional
Tyr in an adjacent loop , provide direct contacts in the interaction with
nidogen . -
pubmed
This
collagen is very insoluble and resistant to the action of depolymerizing agents ( 4 %
EDTA - Na2 ,
pepsin ) . -
pubmed
Thus , the vast majority of the
oligonucleotides synthesized by the 71 core facilities participating in this study were of high quality and performed well as sequencing primers without
post - synthesis purification or desalting . -
pubmed
Finally , our results suggest that an increase in the standard minimum performance specifications of DNA synthesis instruments and
reagents from & gt ; or = 98 % to & gt ; or = 98.5 % average coupling efficiency , or the development of rapid , inexpensive and efficient methods to detect syntheses below the 98.5 % threshold , could obviate
post synthesis purification of sequencing primers . -
pubmed
Acute effects of
bromocriptine ,
cyproheptadine , and
valproic acid on
plasma adrenocorticotropin secretion in
Nelson ' s syndrome . -
pubmed
ACTH was measured every 20 min for 8 h , 2 h before and 6 h after each of the following six treatments : placebo ,
bromocriptine ( 2.5 mg ) ,
cyproheptadine ( 8 mg ) ,
valproic acid ( 1 g ) ,
cyproheptadine plus
valproic acid , and the combination of all three drugs . -
pubmed
The percent maximal suppression of
ACTH after administration of placebo ( 6 + /- 11 % ) ,
cyproheptadine ( 17 + /- 15 % ) ,
valproic acid ( 37 + /- 10 % ) or the combination of
cyproheptadine and
valproic acid ( 19 + /- 14 % ) did not achieve statistical significance . -
pubmed
Bromocriptine , on the other hand , caused a significant decrease in plasma
ACTH ( 52 + /- 8 % ; P & lt ; 0.05 ) , as did the combination of
bromocriptine ,
cyproheptadine , and
valproic acid ( 58 + /- 12 % ; P & lt ; 0.05 ) . -
pubmed
We conclude that at the doses studied ,
bromocriptine had the greatest acute effect in suppressing
ACTH secretion in
Nelson ' s syndrome , and that combined administration with
valproic acid and
cyproheptadine did not further increase this acute
ACTH - suppressive effect . -
pubmed
The adverse course of the disease could not be influenced by repeated haemodialysis nor by the administration of
disodium pamidronate (
Aredia ) and
calcitonin . -
pubmed
The circular dichroism ( CD ) spectrum of the denatured state of
barstar has been analyzed as a function of
urea and temperature . -
pubmed
Water - soluble
2,3,7,8 - tetrachlorodibenzo - p - dioxin complex with
human alpha - fetoprotein : properties , toxicity in vivo and antitumor activity in vitro . -
pubmed
TCDD has been shown to form a stable complex with
AFP in a 2:1 (
TCDD :
AFP ) ratio . -
pubmed
AFP may facilitate
TCDD transport in embryonic tissues and enhance its embryotoxic and teratogenic effects . -
pubmed
The data that imply a role for
BCL2 and related
molecules in such processes are reviewed in this article , which analyzes the factors intrinsic to the neuron that control its capacity for axonal growth . -
pubmed
We investigated several assays to identify and characterize compounds that interfere with the binding of
zinc to
E6 . -
pubmed
METHODS : Thirty - six compounds were selected on the basis of their structure , which would facilitate their participation in
sulfhydryl residue - specific redox reactions , and were tested for their ability to release
zinc from
E6 protein . -
pubmed
The
zinc - ejecting compounds were then tested for their ability to inhibit
E6 binding to
E6 - associated protein (
E6AP ) and
E6 - binding protein (
E6BP ) , two coactivators of
E6 - mediated cellular transformation . -
pubmed
RESULTS : Nine of the 36 tested compounds ejected
zinc from
E6 . -
pubmed
Aspirin ,
heparin ,
low - molecular - weight heparin , hirudin and
GP - IIb / IIIa blockers ] . -
pubmed
The use of
GP - IIb / IIIa blockers , especially the chimeric antibody fragment
abciximab , is well established in interventional cardiology . -
pubmed
Higher - than - standard doses of
mitoxantrone have been shown to augment responses to
cytarabine , possibly by increasing the DNA strand breaks induced by
topoisomerase II ; these strand breaks can not be effectively repaired in the presence of
cytarabine triphosphate . -
pubmed
Toxicity of
PrP106 - 126 may thus be mediated via a sequestration pathway that is not effective for this peptide in
PrP - null cells . -
pubmed
Three of these sites are involved in binding
C3b and regulating complement activation ; others bind to
sialic acid and/or heparin and are responsible for host recognition . -
pubmed
The correlation of gastric emptying and time factors ( age , duration of
diabetes ) and metabolic factors ( blood
sugar level ,
glycosylated haemoglobin ) . -
pubmed
No significant relationship was found between metabolic parameters ( blood
glucose level ,
glycosylated haemoglobin ) and the time of gastric emptying . -
pubmed
The authors did not find a relationship with the blood
glucose level or glycosylated
haemoglobin . -
pubmed
The characteristics of centenarians in Tokyo is 1 ) low level of nutritional parameters , 2 ) low level of
cholesterol and HDL -
cholesterol , 3 ) low level of red blood cells and
hemoglobin , 4 ) high level of
CRP , 5 ) high level of
homocysteine , 6 ) high level of
von Willbrand factor . -
pubmed
The level of
homocysteine is associated with the level of
von Willbrand factor , suggesting that
homocysteine is related to
endothelial injury . -
pubmed
The active site of
metalloproteases is composed of a primary sphere of residues co - ordinating the
zinc atom , and a secondary sphere of residues that determines proteolytic specificity and activity . -
pubmed
Glu-261 and
Glu-267 directly co - ordinates the
zinc atom in
BoNT / A and
BoNT / B respectively and the corresponding residue of
TeNT was replaced by
Asp or by the non conservative residue
Ala. -
pubmed
Tyr-365 is 4.3A away from
zinc in
BoNT / A , and the corresponding residue of
TeNT was replaced by
Phe or by
Ala. -
pubmed
The proteolytic activity of
TeNT -
Asp-271 ( E271D ) is similar to that of the native molecule , whereas that of
TeNT -
Phe-375 ( Y375F ) is lower than the control . -
pubmed
These results demonstrate that both
Glu-271 and
Tyr-375 are essential for the proteolytic activity of
TeNT . -
pubmed
AIM :
Protein tyrosine phosphatase-1B (
PTP-1B ) is an intracellular
PTP known to dephosphorylate and inactivate upstream
tyrosine phosphoproteins in the
insulin signalling cascade . -
pubmed
The aim of the present work was to further elucidate the role of
PTP-1B in
glucose metabolism in vivo . -
pubmed
A hyperinsulinaemic euglycaemic
clamp was used to study whole body
glucose disposal and endogenous
glucose production rates . -
pubmed
Seven days
post injection , no significant difference in rate of
weight gain , fasting blood
glucose or
insulin levels were seen in any group . -
pubmed
Similarly , under steady - state
glucose clamp conditions ,
glucose disposal rate ( R(d ) ) , endogenous
glucose production rate ( EGP ) and
serum insulin levels were similar in all groups . -
pubmed
CONCLUSION : We conclude that moderate medium - term overabundance , to a degree resembling that seen in
insulin - resistant states , of
PTP-1B in liver tissue does not alter
insulin action on
glucose metabolism and that the major site of action of
PTP-1B is presumably at
insulin - responsive target tissue or tissues other than the liver . -
pubmed
A
glutamate residue at the C terminus regulates activity of
inward rectifier K + channels : implication for
Andersen ' s syndrome . -
pubmed
We found that a
glutamate residue of
GIRK2 ( E315 ) , located on a hydrophobic domain of the C terminus , is crucial for the channel activation . -
pubmed
This
glutamate ( or
aspartate ) residue is conserved in all members of the
Kir family . -
pubmed
The mutated
GIRK1 and
GIRK2 retained ion selectivity to
K (+ ) ions . -
pubmed
Methyl - coenzyme M reductase (
MCR ) catalyzes
methane formation from
methyl - coenzyme M (
methyl - SCoM ) and
N-7 - mercaptoheptanoylthreonine phosphate (
CoBSH ) . -
pubmed
The active form of MCR , the
red1 state , is generated by reducing another spectroscopically distinct form called ox1 with
titanium ( III ) citrate . -
pubmed
Previous electron paramagnetic resonance (
EPR ) and
( 14 ) N electron nuclear double resonance ( ENDOR ) studies indicate that both the ox1 and
red1 states are best described as formally
Ni ( I ) species on the basis of the character of the orbital containing the spin in the two
EPR - active species . -
pubmed
RR spectra are also reported for isolated cofactor F(430 ) in the reduced , resting , and oxidized states ; selected RR data are reported for the
( 15 ) N and
( 64 ) Ni isotopomers of the cofactor , both in the intact
enzyme and in solution . -
pubmed
Small
Ni K - edge energy shifts indicate that minimal electron density changes occur at the
Ni center during redox cycling of the
enzyme . -
pubmed
Analyses of the XANES and EXAFS data reveal that both the ox1 and
red1 forms are best described as hexacoordinate and that the main difference between ox1 and
red1 is the absence of an axial
thiolate ligand in the
red1 state . -
pubmed
Furthermore , the vibrational characteristics of the ox1 state and
red1 states are significantly different , especially in
hydrocorphin ring modes with appreciable
C = N stretching character . -
pubmed
It is proposed that these differences arise from a 2 - electron reduction of the
hydrocorphin ring upon conversion to the
red1 form . -
pubmed
Presumably , the ring - reduction and ligand - exchange reactions reported herein underlie the enhanced activity of
MCR(
red1 ) , the only form of
MCR that can react productively with the
methyl group of
methyl - SCoM. -
pubmed
The fetal ductus can be constricted by drugs , including
cyclooxygenase inhibitors (
indomethacin ) ,
nitric oxide synthesis antagonists [
N - nitro - L - arginine monomethyl ester (
L - NAME ) ] , and
glucocorticoid hormones (
dexamethasone ) . -
pubmed
Poorly metabolised
phytanic acid (
PA ) ,
pristanic acid (
PrA ) and
picolenic acid (
PiA ) accumulates in fatty tissues , myelin sheaths , heart , kidneys and retina , leading to
retinitis pigmentosa ,
peripheral dissociative polyneuropathy ,
cerebellar ataxia ( " sailors " walk ) ,
renal , cardiac and liver impairment . -
pubmed
nidulans
hxtA gene was repressed under high
glucose conditions and expressed in vegetative hyphae upon carbon
starvation and during sexual development . -
pubmed
We found
hxtA ( p )::
sgfp expression in developing cleistothecia specifically in ascogenous hyphae and propose that
HxtA is a high affinity
glucose transporter involved in
sugar metabolism during sexual development . -
pubmed
Paired biopsy samples were taken from the esophagus 3 cm above the gastroesophageal junction ; one biopsy was snap - frozen for measurement of
IL-8 mRNA levels by real - time quantitative
polymerase chain reaction , and another was
formalin - fixed for histopathological evaluation . -
pubmed
Lansoprazole treatment significantly reduced the
IL-8 mRNA expression levels . -
pubmed
Arsenic exposure significantly decreased the
glutamate - pyruvate transaminase activity in kidney . -
pubmed
Glucose 6 - phosphatase activity in liver tissue was also significantly decreased after
arsenic treatment . -
pubmed
In addition to these , liver
lactate dehydrogenase activity was elevated due to
arsenic treatment . -
pubmed
Radioligand binding using
[ ( 125 ) I ] cyanopindolol revealed that HF resulted in a comparable decrease in the density of
beta1 - ARs in both surface and T - tubule sarcolemma ( 55+/-4 % , n=7 , P<0.001 ; and 45+/-10 % , n=7 , P<0.01 , respectively ) , but no significant change in
beta2 - AR density was observed . -
pubmed
Whole - cell patch
clamp studies demonstrated a markedly blunted increase in I(
Ca , L ) in response to saturating concentrations of the nonselective
beta - AR agonist
isoproterenol ( 0.1 micromol / L ) in failing myocytes compared with control ( 129+/-20 % , n=11 , versus 332+/-35 % , n=7 ; P<0.001 ) . -
pubmed
Experiments testing
beta1 - AR- and
beta2 - AR - selective stimulation showed that the major component of the blunted response to nonselective
beta - AR stimulation in
HF was caused by
beta2 - AR activation , resulting in a
pertussis toxin - sensitive ,
Gi - mediated inhibition of the
beta1 - AR - induced increase in I(
Ca , L ) . -
pubmed
In parallel , recent studies indicate that
aromatase , the enzyme that converts
testosterone to
estradiol in the brain , is expressed in presynaptic terminals and modulated within minutes by
Ca ( 2 +)-dependent phosphorylation . -
pubmed
Levels of
UDP -
GlcNAc , and the subsequent addition of O - linked
beta - N - acetylglucosamine (
O - GlcNAc ) to
Ser /
Thr residues , is involved in regulating nuclear and
cytoplasmic proteins in a manner analogous to protein phosphorylation . -
pubmed
Recent research has highlighted key roles for
O - GlcNAc serving as a nutrient sensor in regulating
insulin signaling , the cell cycle , and
calcium handling , as well as the cellular stress response . -
pubmed
[ From the Cochrane Library and Diabetes Care : self - monitoring of blood
glucose probably an effective way to improve glycaemic control in patients with
type 2 diabetes not taking
insulin ] . -
pubmed
Self - monitoring of blood
glucose has been found to be effective for patients with
type I diabetes and for patients with
type 2 diabetes taking
insulin . -
pubmed
There is much debate on the effectiveness of self - monitoring of blood
glucose in the management of patients with
type 2 diabetes who are not taking
insulin . -
pubmed
Meanwhile ,
LK-4 ( 3 mg / kg ) significantly inhibited the elevation of plasma
tumor necrosis factor - alpha , as well as values of
GOT and
GPT , and
BUN and
creatinine caused by LPS . -
pubmed
The induction of
inducible NO synthase and the overproduction of
NO and
superoxide anions by LPS were also reduced by
LK-4 . -
pubmed
Screening of several drugs by intradermal treatment prior to toxin injection demonstrated reduced ET - induced vascular leakage with a
cyclo - oxygenase inhibitor (
indomethacin ) , agents that interfere with
histamine (
pyrilamine or
cromolyn ) , or a neurokinin antagonist (
spantide ) . -
pubmed
Systemic administration of
indomethacin or
celecoxib (
cyclo - oxygenase inhibitors ) ,
pyrilamine ,
aprepitant ( a
neurokinin 1 receptor antagonist ) , or
indomethacin with
pyrilamine significantly reduced vascular leakage associated with
ET . -
pubmed
Our results indicate that
ET , acting indirectly or directly on a target yet to be identified , stimulates the production / release of multiple inflammatory mediators , specifically neurokinins ,
prostanoids , and
histamine . -
pubmed
We demonstrated that the HDACI combinations exert different effects on cell cycle arrest and differentiation as determined by
nitro blue reduction and the expression of the early myeloid differentiation marker
CD11b . -
pubmed
The most beneficial effects were found by use of 6 - h pretreatment with
PB and
vitamin B3 before the exposition to
RA alone or in combination with
vitamin B3 , showing significant acceleration and a high level of granulocytic differentiation . -
pubmed
This suggests that interrupting
ET-1 interaction with its
endothelin A ( ET ( A ) ) receptor could be useful for inhibiting
prostate cancer bone
metastasis and , as such , may enhance the therapeutic activity of
docetaxel (
Taxotere ) , the most commonly used drug for the treatment of metastatic
prostate cancer . -
pubmed
Among 40 proteins identified ,
peroxiredoxin 2 (
Prx2 ) was oxidized and 58 kDa
glucose - regulated protein (
GRP58 ) was phosphorylated after 5 min of
Ang II ( 1 microM ) stimulation . -
pubmed
Valsartan , a selective
Ang II type 1 ( AT1 ) receptor blocker , and
N - acetylcysteine , an antioxidant , inhibited both of these modifications , indicating the contribution of
AT1 receptor and reactive
oxygen species to oxidation of
Prx2 and phosphorylation of
GRP58 by
Ang II . -
pubmed
On the other hand ,
LOX metabolites are involved in production of
progesterone , which is required for pregnancy maintenance . -
pubmed
In this study , we evaluated the interaction between the
LOX pathway ,
progesterone , and
HSD11B2 . -
pubmed
Specifically , we hypothesized that
LOX metabolites would alter
HSD11B2 and this effect would be mediated by
progesterone . -
pubmed
By radiometric conversion assay , real - time quantitative PCR , Western blot analysis , and ELISA , we examined
HSD11B2 enzyme activity ,
HSD11B2 mRNA and
HSD11B2 protein expression , and
progesterone output . -
pubmed
Net
progesterone output was increased by
LOX metabolites and decreased by
LOX inhibitors . -
pubmed
Progesterone down - regulated
HSD11B2 activity and
HSD11B2 and
HSD11B2 expression , and these effects were blocked by
RU486 . -
pubmed
Furthermore , the suppressive effect of
12 - HETE on
HSD11B2 activity was also reversed by
RU486 . -
pubmed
Aprepitan , a
neurokinin-1 receptor antagonist , is a centrally active agent that might have the ability to decrease
hot flashes . -
pubmed
Furthermore , the mutation in
TIP neither impaired the
salicylic acid - mediated induction of
HRT expression nor the enhanced resistance conferred by overexpression of
HRT . -
pubmed
Acetohydroxyacid synthase (
AHAS ;
EC 2.2.1.6 ) is the first enzyme in the biosynthetic pathway of the branched - chain
amino acids . -
pubmed
AHAS requires the cofactors
thiamine diphosphate (
ThDP ) ,
Mg ( 2 + ) and
FAD for activity . -
pubmed
AQP1 expression in
TM cells was assessed by
SDS - PAGE and Western blot using anti -
AQP1 IgGs . -
pubmed
Interestingly ,
MPs from ischemic muscles produced more reactive
oxygen species and expressed significantly higher levels of
NADPH oxidase p47 ( 6 - fold ; P<0.05 ) and
p67 subunits ( 16 - fold ; P<0.001 ) than controls , whereas
gp91 subunit expression was unchanged . -
pubmed
We found that
MnSOD activity was increased in
adenocarcinoma , CG and
HP tissues ( p<0.05 - 0.001 ) , while
Cu /
ZnSOD was significantly lower in
adenocarcinoma and
HP tissues ( p<0.001 ) when compared to the healthy control . -
pubmed
The susceptibility of the
face to
hexyl nicotinate ,
sodium lauryl sulfate , and
benzoic acid differed depending on location and age . -
pubmed
carbonic anhydrase and
nitrate reductase ) with an increasing concentration of
Ni . -
pubmed
However , an increase in
proline content and the activities of
catalase ,
peroxidase , and
superoxide dismutase was observed as a result of an increase in
Ni concentration . -
pubmed
The toxic effects generated by
Ni were ameliorated by
HBL through an improved antioxidant system and osmolyte . -
pubmed
CONCLUSIONS : Type , duration , and intensity of physical activity must be considered when planning
carbohydrate replacement and
insulin dose reduction , as must the timing of exercise . -
pubmed
We investigated the associations of
EPHX1 Tyr113His and His139Arg polymorphisms with
asthma and
wheezing outcomes , and focused on the functional genetic change in different ambient
nitrogen dioxide (
NO2 ) levels on
glutathione S - transferase p1 (
GSTP1 ) and
glutathione S - transferase m1 (
GSTM1 ) genotypes . -
pubmed
We examined the associations of
EPHX1 Tyr113His and His139Arg genotypes and diplotypes with
asthma and
wheezing outcomes under different ambient
NO2 exposures . -
pubmed
RESULTS : Children with the
EPHX1 Arg /
His or
Arg /
Arg genotypes at codon 139 were significantly associated with increased risks of lifetime
asthma ( adjusted OR [ aOR ] = 1.3 ; 95 % CI , 1.1 - 1.7 ; and aOR = 1.5 ; 95 % CI , 1.1 - 2.1 , respectively ) . -
pubmed
The risk of
EPHX1 139Arg allele and 113Tyr-139Arg diplotype were of greater magnitude in higher compared with lower
NO2 communities . -
pubmed
The increase of the effect from the
EPHX1 139Arg allele with higher
NO2 exposure was most marked in the
GSTP1 Val allele and
GSTM1 present genotype . -
pubmed
Comparing and contrasting the
( 1 ) H -( 1 ) H correlation data obtained for both polymorphic forms and taking into account the single crystal structure data existing for the solid form
CIP - SAC (
II ) was possible to extract some conclusions on the polymorph
CIP - SAC ( I ) where no single crystal information is available . -
pubmed
Optimized
AR coatings for bulk crystalline
Si and thin - film
CuIn ( 1 - x ) GaxSe ( 2 ) (
CIGS ) solar cells as two representative cases are presented and the effect of solar spectrum in the
AR coating designs is investigated . -
pubmed
By incorporating the transparent conductive and buffer layers as part of the
AR coating in CIGS solar cells ( 2mum - thick
CIGS layer ) , a single
MgF ( 2 ) layer could provide an average reflectance of 8.46 % for wavelengths ranging from 350 nm to 1200 nm and incident angles from 0 to 80 . -
pubmed
RESULTS : Eight compounds were isolated , and their structures were elucidated as
bauerenol ( I ) ,
myristic acid (
II ) ,
11 - phenyl-1 -( 2 ' , 6 '- dihydroxy-4 '- methoxy - phenyl )- undecan-1 - one ( III ) ,
ardisinone A (
IV ) ,
ardisinone B ( V ) ,
ardisinone C ( VI ) ,
ardisinone F ( VI ) ,
beta - sitosterol ( VIII ) . -
pubmed
We , therefore , conclude that an increase in
MAP1S levels triggers autophagy to suppress
genome instability such that both the incidence of
diethylnitrosamine - induced
hepatocarcinogenesis and malignant progression are suppressed . -
pubmed
Hydrolysis of xylan with commercial
xylanase caused breakdown into XOS comprising of
xylobiose ,
xylotriose along with
xylose . -
pubmed
Response surface model ( RSM ) revealed highest
xylobiose yield ( 11 g/100 g xylan ) at pH 5.03 , temperature 45.19 C , reaction time 10.11h with
enzyme dose 17.41 U. -
pubmed
Similarly for maximizing
xylotriose yield , ideal hydrolysis conditions were pH 5.11 , temperature 40.33 C , reaction time 16.55 h with
enzyme dose 13.20 U. -
pubmed
This was confirmed when
cytochrome c release was shown to be unaffected by the pan caspase inhibitor ,
z - VAD - fmk . -
pubmed
Sulfated derivatives based on powdered cellulose were obtained , including those containing additional (
carboxymethyl ,
ethyl amide or
phosphate ) groups , and their activity against
blood clotting factors (
thrombin and
Xa ) was studied . -
pubmed
Lipid peroxidation and
spectrin of RBC membrane in
hydroxyurea treated Ebeta
thalassaemia . -
pubmed
The percentage of
spectrin and the level of
malondialdehyde (
MDA ) ,
fetal hemoglobin (
HbF ) ,
hemoglobin (
Hb ) and packed cell volume ( PCV ) were measured . -
pubmed
The
HbF level was significantly increased after 90 days of
HU therapy . -
pubmed
Different
Ca2 + -sensitivities between the EF - hands of T- and
L - plastins . -
pubmed
Plastins are
Ca ( 2 +)-regulated actin - bundling proteins , and essential for developing and stabilizing
actin cytoskeletons . -
pubmed
Using various spectroscopic methods , gel - filtration chromatography , and isothermal titration calorimetry , we here demonstrate that the EF - hand motifs of both T- and
L - plastin change their structures in response to
Ca ( 2 + ) , but the sensitivity to
Ca ( 2 + ) is lower in
T - plastin than in
L - plastin . -
pubmed
Using experimental approaches , including optical tweezer ( OT ) stretching and atomic force microscopy (
AFM ) indentation , we found that RBCs in the presence of
C ( 60 ) NPs are softer than normal RBCs . -
pubmed
The
strain - stress relations of both normal and
C ( 60 ) infiltrated RBC membranes are extracted from the data of
AFM indentation , from which we proved that
C ( 60 ) NP infiltration can affect the mechanical properties of RBC membrane and tend to weaken the tensile resistance of lipids bilayers . -
pubmed
The influence of
C ( 60 ) NP concentration on the mechanical properties of RBC membrane is successfully predicted . -
pubmed
Insulin resistance and the metabolism of branched - chain
amino acids . -
pubmed
The
BCAAs are particularly responsive to the inhibitory
insulin action on
amino acid release by skeletal muscle and their metabolism is profoundly altered in
insulin resistant conditions and/or
insulin deficiency . -
pubmed
20 - Hydroxyecdysone - induced transcriptional activity of
FoxO upregulates
brummer and acid lipase-1 and promotes lipolysis in Bombyx fat body . -
pubmed
In a previous study , we have shown that the molting hormone ,
20 - hydroxyecdysone (
20E ) , reduces insect food consumption resulting in fat body lipolysis during the non - feeding molting and pupation stages , and assumed that the transcription factor
FoxO is involved in this process . -
pubmed
Three new series of symmetric dimers containing a
naphthoyloxybenzyl (
NB ) ,
benzoyloxynaphthyl (
BN ) , and
naphthoyloxysalicyl (
NS ) mesogenic core linked to an
alkylene spacer via an
imino group were synthesized . -
pubmed
The dimers having
NB and
BN mesogenic units display intercalated smectic structures regardless of the spacer parity . -
pubmed
Great efforts have been undertaken in the last decades to develop new
oximes to reactivate
acetylcholinesterase inhibited by
organophosphorus compounds ( OP ) . -
pubmed
A repeated - measures ANOVA ( group factor : treatment group and control group ; time factor : initial visit , 1.5 , 3 , and 6 months ) was used to analyze the probing depth ( PD ) ,
CAL ,
bleeding on probing (
BOP ) , high - sensitivity
C - reactive protein (
hsCRP ) ,
glycated hemoglobin (
HbA1c ) , and fasting plasma
glucose . -
pubmed
RESULTS : Significantly lower PD ( F=62.898 , P-0.000 ) ,
CAL ( F=51.263 , P-0.000 ) ,
BOP ( F=75.164 , P=0.000 ) ,
hsCRP ( F=6.391 , P=0.010 ) , HbA1c(F=4.536 , P=0.011 ) , and fasting plasma
glucose level ( F= 3.073 , P=0.031 ) were observed after therapeutic periodontal improvement . -
pubmed
Increasing occurrence was found in those formulae produced by the less complex manufacturing processes affecting
casein / whey protein ratio : pre - term , 14.3 % ; starter , 35.3 % ; follow - up , 42.1 % ; toddler 87.6 % ; while hypoallergenic and
lactose - free were totally exempt . -
pubmed
Development of potent antagonists for
formyl peptide receptor 1 based on
Boc - Phe - D - Leu - Phe - D - Leu - Phe - OH . -
pubmed
In this study ,
Boc - Phe - D - Leu - Phe - D - Leu - Phe - OH (
Boc - FlFlF ) , one of several
FPR subtype selective antagonists , was chosen and the positions at the
Phe residues were optimized . -
pubmed
This work describes using phase - angle information from tapping - mode
AFM to follow the interactions of
hydrogen with
palladium ,
polycarbonate , and
iron . -
pubmed
Real - time
AFM phase - angle information allowed for the observation of multiphase mass transfer to and from the surface of
palladium at atmospheric pressure and room temperature without the need for complex sample preparation . -
pubmed
The
AFM observations are quantitatively benchmarked against and confirm mass transfer predictions based on bulk
hydrogen diffusion data . -
pubmed
Each cell nucleus and cytoplasm were identified in relation to
DAPI and
CD99 , respectively , and protein biomarkers ( e.g. -
pubmed
We performed additional testing in two genomic regions (
P2RX4 /
P2RX7 and
P2RY1 ) in a cohort from the Prevalence , Prediction , and Prevention of
Diabetes in Botnia ( n = 3504 ) , which includes more refined measures of
glucose homeostasis . -
pubmed
Rosuvastatin significantly reduced the MPV and the lipid parameters including total
cholesterol ,
triglyceride , and
low - density lipoprotein cholesterol (
LDL - C ) . -
pubmed
However , there was no correlation between MPV and
LDL - C before ( r = -.66 ; P = .383 ) and after ( r = -.112 ; P = .135 )
rosuvastatin treatment or between DeltaMPV and DeltaLDL - C after 40 mg
rosuvastatin daily therapy ( r = -.155 ; P = .073 ) . -
pubmed
An alternative pathway involves the leukocyte
heme protein
myeloperoxidase , which catalyzes the oxidation of
thiocyanate in the presence of
hydrogen peroxide , producing
isocyanate at
inflammation sites . -
pubmed
Recently , it has been shown that carbamylated low - density lipoprotein (
LDL ) induces
endothelial dysfunction via lectin - like - oxidized
LDL receptor-1 activation and increased reactive
oxygen species production , leading to
endothelial nitric oxide synthase uncoupling . -
pubmed
Moreover , carbamylated LDL harbors atherogenic activities , including both binding to
macrophage scavenger receptors inducing
cholesterol accumulation and foam - cell formation , as well as promoting vascular smooth muscle proliferation . -
pubmed
We have demonstrated that , in these nonerythroid cells , adult and embryonic- larval subdomains possess different
DNase I sensitivities and different profiles of H3K27me3 , a
histone modification introduced by PRC2 complex . -
pubmed
They were randomly allocated to receive intravenous
fosaprepitant 150 mg ( n = 19 ;
NK1 group ) or
ondansetron 4 mg ( n = 19 ; ONS group ) before general anesthesia . -
pubmed
IGF-1 level was negatively correlated with
body mass index , waist circumference ( WC ) , AHI , and sleep duration with
oxygen (
O2 ) saturation & lt ; 90 % and positively correlated with the average and minimum
O2 saturation ( p = 0.027 ) . -
pubmed
In a multivariable linear regression , considering WC and minimum
O2 saturation as independent variables , only the minimum
O2 saturation was a predictor of low
IGF-1 levels . -
pubmed
Hemoglobin (
Hb)A1c correlated negatively with the minimum
O2 saturation and
IGF-1 levels . -
pubmed
Therefore , we investigated the pattern of cerebral
glucose metabolism , as well as
dopamine transporter (
DAT ) availability in a large and clinically well - defined cohort . -
pubmed
Testosterone and
androgen receptor gene polymorphism are associated with confidence and competitiveness in men . -
pubmed
We found that
APN downregulated
PTEN expression and that both it and an inhibitor of
PTEN (
SF1670 ) increased the expression of osteogenic markers such as
osteocalcin ,
alkaline phosphatase , and
runt - related transcription factor-2 in
APN - treated hBMSCs . -
pubmed
Whether the specific blood transfer conductance for
nitric oxide (
NO ) with
hemoglobin ( thetaNO ) is finite or infinite is controversial but important in the calculation of alveolar capillary membrane conductance ( DmCO ) and pulmonary capillary blood volume ( VC ) from values of lung diffusing capacity for
carbon monoxide ( DLCO ) and
nitric oxide ( DLNO ) . -
pubmed
To prospectively identify mechanisms through which
HSP90 - dependent
cancer cells evade pharmacologic
HSP90 blockade , we generated multiple mutant
KRAS - driven
cancer cell lines with acquired resistance to the
purine - scaffold
HSP90 inhibitor
PU - H71 . -
pubmed
All cell lines retained dependence on
HSP90 function , as evidenced by sensitivity to short hairpin RNA - mediated suppression of
HSP90AA1 or
HSP90AB1 ( also called
HSP90alpha and
HSP90beta , respectively ) , and exhibited two types of genomic alterations that interfere with the effects of PU - H71 on cell viability and proliferation : ( i ) a Y142N missense mutation in the
ATP - binding domain of
HSP90alpha that co - occurred with amplification of the
HSP90AA1 locus , ( ii ) genomic amplification and overexpression of the
ABCB1 gene encoding the
MDR1 drug efflux pump . -
pubmed
In support of a functional role for these alterations , exogenous expression of
HSP90alpha Y142N conferred PU - H71 resistance to
HSP90 - dependent cells , and pharmacologic
MDR1 inhibition with
tariquidar or lowering
ABCB1 expression restored sensitivity to PU - H71 in
ABCB1 - amplified cells . -
pubmed
Finally , comparison with structurally distinct
HSP90 inhibitors currently in clinical development revealed that PU - H71 resistance could be overcome , in part , by
ganetespib ( also known as
STA9090 ) but not
tanespimycin ( also known as
17 - AAG ) . -
pubmed
Comparison of
everolimus- and biolimus - eluting coronary stents with
everolimus - eluting bioresorbable vascular scaffolds : Two - year clinical outcomes of the EVERBIO
II trial . -
pubmed
We confirmed
serine 14 as a target of
MAPKs and
proline - directed kinases like
cyclin - dependent kinase 5 (
Cdk5 ) in cell - based as well as in vitro
kinase assays and quantitative phosphoproteomic analysis of
TRPC6 . -
pubmed
These two factors - which peak for the best electron donor , the
thiolate ligand - afford a
slim and narrow barrier through which the H - atom can tunnel most effectively . -
pubmed
BACKGROUND / AIMS :
Peptidyl - prolyl cis - trans isomerase FKBP25 is a member of the
FK506 - binding proteins family which has
peptidyl - prolyl cis / trans isomerase domain . -
pubmed
METHODS : The spatio - temporal changes in expression of endothelial
FKBP25 upon
oxygen -
glucose deprivation (
OGD ) treatment were examined by Western blot and immunofluorescence . -
pubmed
Intriguingly ,
FKBP25 nuclear translocation was further validated in
peroxynitrite (
ONOO-)-treated endothelial cells . -
pubmed
PFASs were analyzed in plasma using isotope - dilution ultra - pressure liquid chromatography coupled to tandem mass spectrometry ( UPLC -
MS /
MS ) . -
pubmed
Regulation of
Kv11.1 potassium channel C - terminal isoform expression by the RNA - binding proteins
HuR and
HuD. -
pubmed
She was started on
micafungin , a potent
1,3 - beta - D glucan synthase inhibitor , to broaden antimicrobial coverage when her
insulin requirement decreased to zero for & gt;48 hours . -
pubmed
Right after discontinuation of
micafungin and her switch to a different antifungal ,
insulin requirements increased back to her baseline needs . -
pubmed
Oral administration of linear 1,3 - beta - D glucan has been documented to decrease blood
glucose levels significantly by inhibition of expression of
sodium - glucose transporter 1 (
SGLT1 ) in intestinal mucosa . -
pubmed
INTRODUCTION :
Trebananib , a peptide - Fc fusion protein , blocks angiogenesis by inhibiting binding of
angiopoietin-1 / 2 to the
receptor tyrosine kinase Tie2 . -
pubmed
Trebananib plus
trastuzumab and
paclitaxel was evaluated in
human epidermal growth factor receptor 2 - positive
breast cancer in an open - label phase 1b clinical study . -
pubmed
CONCLUSIONS : This case indicated an association of
BRCA2 mutation with high mutation loads and an excellent response of
oxaliplatin - based chemotherapy regimen for LARC . -
pubmed
Collectively , these results suggest that
LSD2 regulates distinct sets of genes during brown and beige adipocyte formation.-Takase , R. , Hino , S. , Nagaoka , K. , Anan , K. , Kohrogi , K. , Araki , H. , Hino , Y. , Sakamoto , A. , Nicholson , T. B. , Chen , T. , Nakao , M.
Lysine - specific demethylase-2 is distinctively involved in brown and beige adipogenic differentiation . -
pubmed
A
monosaccharide ( mainly
glucose ) was also obtained from the enzymatic saccharification of thermal acid hydrolysate using 16 U / mL Celluclast 1.5 L
enzyme at 45 C for 36 h. Detoxification was performed using the adsorption method with activated
carbon , the overliming method with
Ca ( OH ) 2 , and the ion exchange method with
polyethyleneimine . -
pubmed
We performed a randomized trial to assess whether the combination of NSAIDs and sublingual
nitrate is more effective than NSAIDs alone in preventing
PEP . -
pubmed
This finding was further substantiated by varying
Pb2 + loadings on
carbon nanoparticles and overexpressing
lnc - PCK1 - 2:1 . -
pubmed
The
ira2 isu1 double deletion led to
strains with the highest specific
xylose consumption- and
ethanol production rates but also the lowest biomass titre . -
pubmed
The signalling response revealed that
ira2 isu1 changed the low
glucose - signal in the background
strain to a simultaneous signalling of high and low
glucose , suggesting that engineering of the signalome can improve
xylose utilization . -
pubmed
Furthermore , the expression of
beta - catenin , p -
AKT ,
VEGF ,
MMP-2 and
MMP-9 proteins could be down - regulated after SMA /
CORM-2 treatment . -
pubmed
SMA /
CORM-2 exerted both in vitro and in vivo anti - proliferation and anti - metastatic effects , which may yield a novel therapeutic strategy for
CRC . -
pubmed
On the other hand , in
MBL and DBF forests , the ambient dose rate decreased 10 - 20 % faster than that induced solely by physical decay of
radiocesium for the observation period 2011 - 2016 . -
pubmed
All
formalin - fixed , paraffin - embedded blocks from the surgical specimens from each subject were sectioned and stained with
hematoxylin and eosin and antibodies to
tau ( Thermo Fisher Scientific Clone AT8 ) , and examined blindly for tau pathology , including lesions characteristic of
CTE . -
pubmed
Low - Fouling Electrosprayed
Hemoglobin Nanoparticles with Antioxidant Protection as Promising
Oxygen Carriers . -
pubmed
Despite all the attempts to create advanced
hemoglobin (
Hb)-based
oxygen carriers ( HBOCs ) employing an encapsulation platform , major challenges including attaining a high Hb loading and long circulation times still need to be overcome . -
pubmed
Finally , the Hb - NPs also preserve the reversible
oxygen - binding and releasing properties of
Hb . -
pubmed
MicroRNA-21 expressions were evaluated using reverse transcription -
polymerase chain reaction ( RT - PCR ) and osteogenesis was detected via
Alizarin Red S (
ARS ) staining and measurement of
alkaline phosphatase (
ALP ) activity . -
pubmed
Sortase - Mediated Site - Specific Modification of
Interleukin-2 for the Generation of a
Tumor - Targeting
Acetazolamide -
Cytokine Conjugate . -
pubmed
Site - specific conjugation between
interleukin-2 and
acetazolamide was obtained by
sortase A - mediated transpeptidation . -
pubmed
Optimization of manufacturability and
tumor - targeting performance of
acetazolamide -
cytokine products will be required in order to enable industrial applications . -
pubmed
METHODS : We induced increased
O - GlcNAc by treating
AD and control cybrid cell lines with
Thiamet G (
TMG ) , an inhibitor of the
O - GlcNAcase enzyme that mediates removal of the nutrient - dependent
O - GlcNAc modification . -
pubmed
At baseline ,
AD cybrid cell line mitochondria showed partial activation of several proteins that help maintain bioenergetic homeostasis such as
AMP - Regulated Kinase suggesting that
AD mitochondria initiate a state of nutrient stress promoting energetic compensation ; however , this compensation reduces the capacity of cells to respond to additional nutrient - related stresses such as
TMG treatment . -
pubmed
Also ,
TMG caused disruptions in acetylation and
Sirtuin 3 expression , while lowing total energetic output of the cell . -
pubmed
Ketogenic diet and
ketone bodies enhance the anticancer effects of
PD1 blockade . -
pubmed
Supplementation of KD with
sucrose ( which breaks ketogenesis , abolishing
3HB production ) or with a pharmacological antagonist of the
3HB receptor GPR109A abolished the antitumor effects . -
pubmed
Mechanistically ,
3HB prevented the ICB - linked upregulation of
PD - L1 on myeloid cells while favoring the expansion of
CXCR3 + T cells . -
pubmed
Methylene Blue (
MB ) was used as an organic model compound . -
pubmed
Breakage of the aromatic ring of MB appeared by the scission of the double
C - S + C bond as a result of the attack of
OH species on adsorbed and electronically reorganized
MB molecule . -
pubmed
Previous studies have found that
tomatidine decreases hepatic lipid accumulation via regulation of
vitamin D receptor and activation of
AMP - activated protein kinase (
AMPK ) phosphorylation . -
pubmed
In vivo and in vitro ,
tomatidine significantly suppressed the expression of
fatty acid synthase and transcription factors involved in lipogenesis , and increased the expression of
adipose triglyceride lipase . -
pubmed
Tomatidine promoted the
sirtuin 1 (
sirt1)/
AMPK signaling pathway to increase lipolysis and beta - oxidation in
fatty liver cells . -
pubmed
These findings suggest that
tomatidine potentially ameliorates
obesity and acts against
hepatic steatosis by regulating lipogenesis and the
sirt1 /
AMPK pathway . -
pubmed
While
pyruvate dehydrogenase was inhibited in vitro ,
halofenate also inhibited the activety of various other isolated enzymes . -
pubmed
Role of endogenous
somatostatin in the secretion of
parathyroid hormone and
calcitonin . -
pubmed
Our previous in vitro and in vivo studies demonstrated that exogenous
somatostatin inhibited secretion of both
parathyroid hormone (
PTH ) and
calcitonin (
CT ) . -
pubmed
This study evaluates the possible role of endogenous
somatostatin in
PTH and
CT secretion . -
pubmed
These combined observations strongly suggest that endogenous
somatostatin must have a suppressive effect on
PTH and
CT secretion . -
pubmed
These data strongly suggest that
somatostatin is a locally - synthesized hormone that has a role in modulation of both
PTH and
CT secretion . -
pubmed
Experiments in vivo indicate that N - acetylation may be a possible alternative metabolic pathway for biogenic
amines and that
brain N - acetyltransferase activity may be regulated by a beta - adrenergic mechanism . -
pubmed
These results suggest that
potassium ion may function on the affinities of
dihydropteroate and
L - glutamate to the
enzyme . -
pubmed
Dihydrofolate synthetase was inhibited by the addition of reduced forms of
homopteroic acid . -
pubmed
When atherogenic indices ( such as the relationship between total
cholesterol and
HDL -
cholesterol or the
Apo A1 /
apo B quotient ) are used , 20 to 30 % of an
IDDM population considered to be in clinically acceptable control have to be considered pathological . -
pubmed
This observation is even more important since the recent Diabetes Control and Complications Trial has shown that , especially in the younger group of patients , significantly higher total
cholesterol and
triglycerides and lower
HDL - cholesterol were observed . -
pubmed
In this work , the helix - forming residues in fragments of several proteins (
ribonuclease ,
thermolysin ,
tendamistat and
angiogenin ) were identified by NOE and the helix proton shifts were measured as delta changes associated with helix - population increments driven by
trifluoroethanol addition . -
pubmed
Aromatic residues considerably distorted the periodicity of the helix
amide shifts in some peptides , as evidenced by the delta shifts of a
RNase A fragment 1 - 15 analog in which the two aromatic residues were replaced by
Ala. -
pubmed
The
benzodiazepine receptor antagonist
ZK 93426 blocks the capacity of
amitriptyline to augment the uptake of
36Cl- by 30 microM
GABA . -
pubmed
Our results support a model whereby dorso - ventral mesodermal patterning can be attained by a single mesoderm inducing
agent , possibly
bFGF , which is uniformly distributed across the prospective dorsal - ventral axis , and which acts in concert with a dorsally localized signal , possibly a Wnt protein , which either alters the response of ectoderm to induction or modifies the character of mesoderm after its induction . -
pubmed
Peptide
histidine valine (
PHV ) is a 42
amino acid polypeptide closely related to the neuropeptides
VIP ,
PHI and
PHM . -
pubmed
The lack of effect of
PHV infusion on blood pressure , despite
tachycardia and marked cutaneous vasodilatation , implies that PHV has a different effect on the resistance vessels from that of other peptides such as
VIP . -
pubmed
Receptors ,
growth factors and
steroid insensitivity of
tumours . -
pubmed
Throughout the physiologic range of plasma
insulin concentrations , both the increase in total body
glucose uptake and the suppression of hepatic
glucose production ( HGP ) were significantly impaired in the
obese group ( P less than .001 to .01 ) . -
pubmed
At the two highest plasma
insulin concentrations , inhibition of
HGP and the stimulation of
glucose disposal were similar in both the
obese and control groups . -
pubmed
One high - affinity site is the common
DM1 /
sigma 1 site , the affinity of which is allosterically increased 4 to 5 - fold by 10 microM
ropizine . -
pubmed
The Kd values of the
DM1 / sigma 1 for DM and
( + ) - 3 - PPP are 17 and 11 nM respectively . -
pubmed
DM binds to the second high - affinity site (
R2 ) with a Kd of 15 nM ; this site has low affinity for
( + ) - 3 - PPP . -
pubmed
Conversely ,
( + ) - 3 - PPP binds with high affinity ( Kd 53 nM ) to another site (
R3 ) , that has low - affinity for DM . -
pubmed
The cells were stained with monoclonal antibodies to
ICP4 ,
ICP5 ,
ICP6 ,
ICP8 , and
gB , using the
avidin -
biotin - peroxidase complex (
ABC ) method . -
pubmed
[ The effect of the
beta 1 selective beta blocker ,
betaxolol , on metabolism in
type II diabetics ] . -
pubmed
No significant alterations were observed in postprandial blood
glucose ,
glycosylated haemoglobin and 24 - h urinary
glucose excretion . -
pubmed
The lack of noticeable influence on the
carbohydrate metabolism during 4 - week therapy is presumably related to the high
beta 1 - selectivity of
betaxolol . -
pubmed
No correlation between
ILS - induced paroxysmal activity and a decrease in
TTP contents was found . -
pubmed
Na +/ K +- ATPase activity was determined by measuring the inorganic
phosphate ( Pi ) released by incubation in a reaction medium in the presence and absence of K ions or
ouabain . -
pubmed
Fasting total plasma
cholesterol ,
triglycerides ,
high density lipoprotein cholesterol (
HDL ) , and
body mass index were also measured . -
pubmed
Estimates were similar for heritability of
HDL cholesterol ( 51 vs. 45 % ) . -
pubmed
No common household effect ( except for adjusted
HDL cholesterol ( 24 % ) , p less than 0.01 ) was statistically significant for the lipids , blood pressures , or
body mass index . -
pubmed
Spouse correlations for lipids , blood pressures , and
body mass index were low , with a maximum of 0.12 ( p less than 0.05 ) for
HDL cholesterol . -
pubmed
blocked both
acetylcholine- and neurokinin B- , but not
substance P- or neurokinin A - induced
salivation . -
pubmed
, a putative
substance P antagonist , reduced responses to mammalian neurokinins but caused a 2 - fold potentiation of
acetylcholine - induced
salivation . -
pubmed
, a novel putative
neurokinin B antagonist , significantly reduced
substance P- and neurokinin B- but not
acetylcholine- or neurokinin A - induced
salivation . -
pubmed
Liver microsomal inactivation of
4 - methyl-5 - amino-1 - formylisoquinoline thiosemicarbazone as an inhibitor of
ribonucleotide reductase . -
pubmed
Studies were carried out to determine the effects of preincubation of
4 - methyl-5 - amino-1 - formylisoquinoline thiosemicarbazone (
MAIQ ) with hepatic microsomes on the ability of MAIQ to inhibit
CDP reductase activity in vitro . -
pubmed
When MAIQ ( 5 microM ) was first incubated in the presence of hepatic microsomes and
NADPH ,
CDP reductase activity was inhibited only 30 % . -
pubmed
This attenuation of
MAIQ inhibition was dependent on time of incubation and microsomal
protein concentration and showed an obligatory requirement for
NADPH or
NADH . -
pubmed
In addition , a significant positive correlation was found between serum
testosterone levels and
LH B / I ratios in the impotent men ( r = 0.45 ; P = 0.029 ) . -
pubmed
This paper reports the results of administration of a course of three 1 ml intramuscular injections of
anti - HBs immunoglobulin to the babies of
HBsAg positive mothers recognised during pregnancy over a period 1982 - 1985 . -
pubmed
However , when comparing measurements made during
mexiletine treatment with those at baseline ,
mexiletine monotherapy increased only the refractory periods of
S4 . -
pubmed
The effective refractory period of
S4 during
mexiletine monotherapy ( 200 + /- 20 ms ) was significantly longer than at baseline ( 160 + /- 21 ms ) . -
pubmed
The effective refractory period of
S4 during combination therapy ( 253 + /- 26 ms ) was significantly longer than that of
quinidine monotherapy ( 223 + /- 27 ms). -
pubmed
Phorbol myristate acetate (
PMA ) suppresses polarization and locomotion and alters
F - actin content of
Walker carcinosarcoma cells . -
pubmed
Changes in shape and chemokinesis of
Walker carcinosarcoma cells were associated with alterations in the relative amount and the topographical distribution of
F - actin as determined by
NBD - phallacidin binding . -
pubmed
Suppression by
PMA was associated with loss of the polar topographical distribution of
F - actin visualized by
NBD - phallacidin binding . -
pubmed
In the presence of
PMA , the relative amount of
F - actin was higher than in unstimulated controls and lower in cells exposed to microtubule - disassembling agents . -
pubmed
1,4 - Bis [ 2 -( 3,5 - dichloropyridyloxy )] benzene (
TCPOBOP ) and related compounds as inducers of hepatic
monooxygenases . -
pubmed
Ten analogs of
TCPOBOP have been synthesized and their activities as microsomal
enzyme inducers evaluated . -
pubmed
The results illustrate that changes in the structure of the
pyridyloxy ring markedly affect
enzyme induction activity . -
pubmed
In addition , the effects of altered substitution pattern of the
benzene ring and structural alterations of the internal ring moiety were evaluated by measuring hepatic microsomal
coumarin hydroxylase activity . -
pubmed
The results confirm the microsomal
monooxygenase enzyme induction activity of
TCPOBOP , and the observed structure - dependent potencies of several related homologs support a receptor - mediated mechanism of action for the process . -
pubmed
Subcellular localization of the
adrenal desmolase active in the formation of
dehydroepiandrosterone (
3 - hydroxy-5 - androsten-17 - one ) . -
pubmed
A synthetic dodecapeptide corresponding to the predicted reading frame of the repeating
nucleotide unit behaved , in an immunoradiometric assay , identically with the native
P. - pubmed
Circulating
angiotensin II and
adrenal receptors after nephrectomy . -
pubmed
Mineralocorticoid secretion is predominantly controlled by the octapeptide
angiotensin II , which exerts trophic actions on the adrenal glomerulosa and acute regulatory effects on
aldosterone biosynthesis . -
pubmed
The trophic actions include stimulation of
angiotensin II receptors and enzymes of the
aldosterone biosynthetic pathway , with corresponding enhancement of the
aldosterone secretory capacity of the adrenal gland . -
pubmed
The positive regulatory action of
angiotensin II on its
adrenal receptors occurs with elevations of the circulating peptide concentration within the physiological range and probably contributes to the increased sensitivity of the adrenal during
sodium deficiency . -
pubmed
To clarify these conflicting views we evaluated the effects of nephrectomy on adrenal
angiotensin II receptors in relation to blood
angiotensin II and plasma
electrolyte levels . -
pubmed
Maltose at a concentration of 0.5 % stimulated the
glucoamylase synthesis , but at a concentration of 4 % it repressed not only the enzyme synthesis but the growth of the parental
strain on the
agar medium . -
pubmed
The mutant 66 produced about 2.9 times more
glucoamylase than its parent when
maltose was added at 0.5 % concentration to the medium . -
pubmed
The addition of
cAMP ( 5.10(-5 ] resulted in a partial release of
maltose ( 4 % ) repression of the
glucoamylase synthesis in both
strains . -
pubmed
These results suggest that
thyroid hormone influences the
cholesterol esterification through the activation of
lecithin : cholesterol acyltransferase . -
pubmed
Kinetic modeling indicates that these
NMDA receptors open at a rate of 230 s-1 and close at a rate of 167 s-1 , that in the absence of desensitization the maximum probability of being open is approximately 0.5 , and that this probability is half - maximal at approximately 150 microM
NMDA . -
pubmed
The results indicate that heparin and
fucoidan , a naturally occurring sulfated
L - fucose polymer , suppress the formation of an initial non - covalent interaction between 125I -
TGF - beta 1 and activated
alpha 2 - M. -
pubmed
Electrophoresis of 125I -
TGF - beta 1 showed that
fucoidan protects
TGF - beta 1 from proteolytic degradation by
plasmin and
trypsin . -
pubmed
While
plasmin caused little , if any , activation of latent
TGF - beta derived from vascular smooth muscle cells ( SMC ) ,
plasmin degraded acid - activated
TGF - beta , and purified
TGF - beta 1 , and this degradation was inhibited by
fucoidan . -
pubmed
In vitro , heparin and
fucoidan tripled the half - life of 125I -
TGF - beta 1 and doubled the amount of cell - associated 125I -
TGF - beta 1 . -
pubmed
p35 , a major antigen extracted from the membrane of this mycoplasma using
Triton X-114 has been found to be a lipoprotein . -
pubmed
Propranolol increased the density of cerebral
beta - adrenoceptors by 77 % and reduced the downregulation induced by stress . -
pubmed
Atenolol also increased the density of cerebral
beta - adrenoceptors by 34 % and abolished the stress - induced downregulation in cerebral
beta - adrenoceptor density . -
pubmed
These results suggest that the antihypertensive action of
propranolol and
atenolol may be partly associated with the inhibition of stress - activated central
beta - adrenoceptor transmission . -
pubmed
The effect of the concentration of
peptone , as an additive , was investigated on cell growth ,
monoclonal antibody formation , and cell metabolism over 0 - 10 g l-1 range . -
pubmed
It was found that 1 - 5 g l-1 peptone can significantly promote the growth of cells and increase the formation of
monoclonal antibody , especially at 3 - 5 g l-1 , when both the accumulating level and secretion rate of
monoclonal antibody are higher than that at other
peptone concentrations . -
pubmed
The cell growth and
monoclonal antibody formation were inhibited at higher
peptone concentrations , e.g. -
pubmed
The proteolytic activity of the enzyme against dimethyl casein was inhibited by
ethylenediaminetetraacetic acid and
alpha 2 - macroglobulin . -
pubmed
Demonstration of the suitability of
CGP 12177 for in vivo studies of
beta - adrenoceptors . -
pubmed
Injection of ( + /- ) -
propranolol at 15 min displaced ~ 60 % of the radioactivity by 90 min indicating that the tracer binds to
beta - adrenoceptor sites in vivo.6 . -
pubmed
The results show that
( -)-[ 3H ]- CGP 12177 is a suitable ligand for assessing
beta - adrenoceptors in vivo . -
pubmed
Because
somatostatin also prevents lymphocyte proliferation , we hypothesized that iIELs may influence production of
somatostatin . -
pubmed
Concentration maps of paramagnetic tracers
Gd3 + were measured in time through direct measurements of spin lattice relaxation time
T1 obtained by using a two - point stimulated echo
pulse sequence . -
pubmed
Based upon known morphology , we suggest that the only structure supporting
triad position is the
SR itself , and that a non - homogeneous distribution of the
SR within the sarcomere might be responsible for maintaining
triad location near the mid - region of the thin filaments . -
pubmed
Determination of
5 - hydroxytryptophol in urine by high - performance liquid chromatography : application of a new
post - column derivatization method with fluorometric detection . -
pubmed
The eluate was injected onto an isocratically eluted
C18 reversed - phase column and
5HTOL was converted into a fluorescent
oxazole derivative by on - line
post - column reaction with
benzylamine in the presence of
potassium hexacyanoferrate ( III ) . -
pubmed
Removal of
adenosine decreases the responsiveness of muscle
glucose transport to
insulin and contractions . -
pubmed
In the heart and in adipocytes ,
adenosine potentiates
insulin - stimulated
glucose transport . -
pubmed
There is controversy regarding the effect of
adenosine in skeletal muscle , with reports of both an inhibitory effect and no effect , on
insulin - stimulated
glucose transport . -
pubmed
Measurement of the increase in
GLUT4 at the cell surface in response to a maximally effective
insulin stimulus in the epitrochlearis muscle , using the exofacial label
ATB -[ 3H ] BMPA , showed that
adenosine deaminase treatment markedly reduces cell - surface
GLUT4 labeling . -
pubmed
The reduction in cell - surface
GLUT4 labeling was similar in magnitude to the decrease in maximally
insulin - stimulated
glucose transport activity in
adenosine deaminase - treated muscles . -
pubmed
These results show that
adenosine potentiates
insulin- and contraction - stimulated
glucose transport in skeletal muscle by enhancing the increase in
GLUT4 at the cell surface and raise the possibility that decreased
adenosine production or action could play a causative role in
insulin resistance . -
pubmed
The effects of intraarticular
bupivacaine and
adrenaline , and a comparison of synovial fluid cell and synovial membrane
cytokine expression , were also investigated . -
pubmed
The
xanthone 6 - hydoxylases are
cytochrome P450 monooxygenases , as shown by their dependence on
NADPH and molecular
oxygen and their inhibition by
carbon monoxide and typical
P450 inhibitors . -
pubmed
In both cell cultures ,
xanthone accumulation was preceded by an increase in
xanthone 6 - hydroxylase activity . -
pubmed
Cells were activated by
phorbol myristate acetate (
PMA ) and
anti - CD3 ,
PMA -
ionomycin , or lipopolysaccharide ( LPS ) and assessed for cytokine mRNA transcription by semiquantitative reverse transcriptase -
polymerase chain reaction , intracellular protein production by flow cytometry , and secretion by supernatant ELISA . -
pubmed
CF cells secreted less
IFN - gamma than control cells after maximal activation by
PMA -
anti - CD3 ( 1836 + /- 273 pg / ml versus 9635 + /- 3437 pg / ml , P = 0.04 ) . -
pubmed
A promising approach to the gene therapy of
PD is intrastriatal expression of
dopamine - synthesizing enzymes [
tyrosine hydroxylase (
TH ) and
aromatic L - amino acid decarboxylase (
AADC ) ] . -
pubmed
Combination of
dopamine - supplement gene therapy and
GDNF gene therapy would be a logical approach to the treatment of
PD . -
pubmed
All ten of the possible five -
carbon acyclic primary beta- , gamma- , and
delta - amino alkanoic acids (
amino position isomers of the
valines ) have been positively identified in hot - water extracts of the Murchison meteorite using combined gas chromatography - mass spectrometry (
GC -
MS ) and ion exchange chromatography . -
pubmed
Fifteen months after deworming with
albendazole and
praziquantel the prevalence of
anaemia was reduced by a quarter and that of moderate - to - severe
anaemia (
haemoglobin & lt;90 g / l ) was reduced by nearly a half . -
pubmed
The maintenance of
oxygen supply to the tissues during
anaemia involves complex cardiovascular adaptations , including an increase in cardiac output , reduced peripheral resistance and increased
oxygen extraction from
haemoglobin (
Hb ) . -
pubmed
Many potential reasons for
erythropoietin - induced
hypertension in
uraemic patients have been postulated , including increased blood viscosity as haematocrit rises , a reversal of hypoxic vasodilatation , increased blood volume that is not compensated by haemodialysis , ultrafiltration and impaired
nitric oxide synthesis , preventing vascular relaxation in response to increased blood viscosity . -
pubmed
Similarity with wild - type receptors has also been demonstrated by the differential insertion of
insulin - bound and free
HIR - MAED complexes into artificial bilayer membranes of
Triton X-114 . -
pubmed
[ Stress -
rest myocardial tomoscintigraphy with
99mTc - tetrofosmin in the diagnosis of
ischemic disease ] . -
pubmed
injections of
99mTc tetrofosmin , one at peak exercises ( 370 MBq ) and the other ( 740 MBq ) at
rest 3 h after exercise ( images 15 - 30 min after injections for both studies ) . -
pubmed
The neointimal area at 28 days was not diminished , however , by
NO eluded from either stents of thin or thick barriers ( control bare stent - 0.66 mm2 , control PU stent - 0.68 mm2 ,
SNP - PU thin coating stent - 0.78 mm2 ,
SNP - PU thick coating stent - 0.85 mm2 ; all p =
NS ) . -
pubmed
The clinical data with
ranolazine focuses on its use in
chronic stable angina , where it has been shown to increase exercise tolerance and decrease
angina compared with placebo and in combination with beta - blockers and
calcium - channel blockers . -
pubmed
Asp177 , conserved in all PP(i )
PFKs , negates the binding of the
alpha - phosphate group of
ATP and likely contacts the essential
Mg ( 2 + ) cation via a water molecule . -
pubmed
Mesenteric /
SAF ratio of
IL-6 ,
TNF - alpha ,
corticosterone , and gene profiling showed decrease of
inflammation after RYGB . -
pubmed
The combination of
indole-3 - acetic acid (
IAA ) and
horseradish peroxidase (
HRP ) has recently been proposed as a novel
cancer therapy . -
pubmed
It is known that
IAA produces free radicals when oxidized by
HRP . -
pubmed
Because oxidative stress could induce apoptosis , we measured the production of free radicals at varying concentrations of
IAA and
HRP . -
pubmed
Our results show that
IAA /
HRP produces free radicals in a dose - dependent manner , which are suppressed by
ascorbic acid or
( -)- epigallocatechin gallate (
EGCG ) . -
pubmed
In addition ,
IAA /
HRP was observed to activate
p38 mitogen - activated protein ( MAP ) kinase and
c - Jun N - terminal kinase (
JNK ) , which are almost completely blocked by antioxidants . -
pubmed
We further investigated the
IAA /
HRP - mediated apoptotic pathways , and found that
IAA /
HRP activates
caspase-8 and
caspase-9 , leading to
caspase-3 activation and
poly ( ADP - ribose ) polymerase (
PARP ) cleavage . -
pubmed
IH induced significant decreases in
Ser-133 phosphorylated
CREB (
pCREB ) without changes in total
CREB , starting as early as 1 h IH , peaking at 6 h-3 days , and returning toward normoxic levels by 14 - 30 days . -
pubmed
In contrast , the presence of 5
Chordin - like
cysteine - rich domains in the
CV-2 protein suggests that
CV-2 belongs to a family of well - established
inhibitors of
BMP function that includes
Chordin and
Sog [ Development 127 ( 2000 ) 3947 ] . -
pubmed
The LPS - induced inhibition of the neurogenic vasopressor response was counteracted by the
cannabinoid CB ( 1 ) receptor antagonist
SR 141716A ( 0.1 micromol x kg(-1 ) ) , but not by the
CB ( 2 ) receptor antagonist
SR 144528 ( 3 micromol x kg(-1 ) ) , the
vanilloid VR1 receptor antagonist
capsazepine ( 1 micromol x kg(-1 ) ) or the
histamine H ( 3 ) receptor antagonist
clobenpropit ( 0.1 micromol x kg(-1 ) ) . -
pubmed
We conclude that in the initial phase of
septic shock , the activation of presynaptic
CB ( 1 ) receptors by endogenously formed
cannabinoids contributes to the inhibition of the neurogenic vasopressor response . -
pubmed
At various time points postrelease , matrix lysates were blotted for the proteins of interest , and the terminal
deoxynucleotidyltransferase - mediated
dUTP nick - end label assay was performed on both whole matrices and cytospin preparations . -
pubmed
RESULTS :
Terminal deoxynucleotidyltransferase - mediated
dUTP nick - end label positivity in attached versus detached matrices ( at 24 h
post detachment ) was 0.7 + /- 03 versus 5.3 + /- 1.7 % ( P & lt ; 0.05 , unpaired t test ) . -
pubmed
Biotin - rich , optically clear nuclei express
estrogen receptor - beta :
tumors with morules may develop under the influence of
estrogen and aberrant
beta - catenin expression . -
pubmed
We investigated a possible role of
estrogen receptor ( ER )- beta in the occurrence of
tumors that form morules with
biotin - rich optically clear nuclei ( BROCN)(BROCN - family
tumors ) and its possible relationship with aberrant nuclear / cytoplasmic ( N / C )
beta - catenin expression . -
pubmed
Whether the
estrogen - signaling pathway and the
Wnt - signaling pathway have crosstalk , cooperating in the development of the BROCN - family
tumors , awaits further study . -
pubmed
Isoflavone synthase (
IFS ) catalyzes the key entry point step of
isoflavone biosynthesis from the general
phenylpropanoid pathway . -
pubmed
The defense related hormone
salicylic acid induced the expression of
IFS1 :
GUS in all cell types of the root . -
pubmed
We estimated the relative risk ( RR ) for each lipid parameter , adjusted for
C - reactive protein ,
homocysteine ,
body mass index , family history ,
hypertension ,
diabetes , postmenopausal hormone use , physical activity ,
alcohol intake , and blood draw parameters . -
pubmed
The RRs associated with an increase of approximately 1 SD ( mg / dL ) were as follows :
HDL cholesterol (
HDL - C ) ( RR=0.6 [ 0.5 to 0.8 ] , SD=17 ) ,
apolipoprotein B100 (
apoB100 ) ( RR=1.7 [ 1.4 to 2.1 ] , SD=32 ) ,
LDL cholesterol (
LDL - C ) ( RR=1.4 [ 1.1 to 1.7 ] , SD=36 ) , total
cholesterol (
TC ) ( RR=1.4 [ 1.1 to 1.6 ] , SD=40 ) , and
triglycerides ( RR=1.3 [ 1.0 to 1.5 ] , SD=80 ) . -
pubmed
After simultaneous control for several lipid biomarkers ,
HDL - C was the primary contributor of the variation in multivariate models ( P=0.01 ) , followed by
LDL - C ( P=0.01 ) , whereas
triglycerides and
apoB100 did not contribute further information . -
pubmed
The
hepatitis C virus ( HCV ) RNA - dependent RNA polymerase (
RdRp ) has several distinct biochemical activities , including initiation of RNA synthesis by a de novo mechanism , extension from a primed template , nontemplated
nucleotide addition , and synthesis of a recombinant RNA product from two or more noncovalently linked templates ( template switch ) . -
pubmed
While the prototype
enzyme had a K(m ) for
GTP of 3.5 microM , all of the mutations except one had K(m)s that were three- to sevenfold higher . -
pubmed
Using
biotin-4 -
fluorescein , the detection limit of
streptavidin lies in the same range as with
Coomassie blue staining . -
pubmed
Based on well - controlled clinical trials , vasodilators such as
misoprostol , and
histamine receptor ligands should be further investigated . -
pubmed
Finally , given the evidence that
ST is a form of
sensory epilepsy , new antiepileptic drugs should be tested for potential efficacy as they are developed ; such drugs may include novel
N - methyl - D - aspartate receptor antagonists , as well as
cannabinoids . -
pubmed
These foci colocalized with those of
Rad51 and
phospho - DNA -
PKcs . -
pubmed
Furthermore ,
gammaH2AX and
phospho - DNA -
PKcs foci were localized at expressed fragile sites on metaphase chromosomes . -
pubmed
Intergastric treatment with
NAC or
SMC five times a week resulted in decreased numbers and areas of preneoplastic ,
glutathione S - transferase placental form (
GST - P ) positive foci of the liver in a dose - dependent manner . -
pubmed
Moreover , cell proliferation was reduced in
GST - P positive foci by
NAC and SMC . -
pubmed
Insulin - like growth factor I (
IGF - I ) and
inducible nitric oxide synthase (
iNOS ) mRNA expressions were found downregulated in the liver by
NAC . -
pubmed
Programme attendance :
CBT +
Acamprosate group ( 66.1 % ) ,
CBT +
Naltrexone group ( 79.7 % ) , and in the
CBT + Combined group ( 83.1 % ) . -
pubmed
RESEARCH METHODS AND PROCEDURES : Isolated retroperitoneal adipocytes were cultured for 0.5 to 48 hours without ( baseline ) or with ( 0.001 to 1 nM )
ghrelin alone or in combination with
insulin ( 0.01 to 10 nM ) or
dexamethasone ( 1 to 100 nM ) . -
pubmed
Adipocytes were also incubated with
ghrelin and inhibitors either of RNA (
actinomycin D ) or protein synthesis (
cycloheximide ) or with several concentrations ( 10 to 1000 nM ) of a specific
ghrelin antagonist . -
pubmed
RESULTS : Our data indicate that ghrelin directly enhanced adipocyte
leptin release and ob mRNA expression , that the
leptin - releasing activity of ghrelin was additive to the action of both
insulin and
dexamethasone and was abrogated by protein synthesis inhibitors , and that effects of ghrelin on adipocyte ob mRNA expression and release were blocked by coincubation with the specific
growth hormone secretagogue receptor 1a antagonist . -
pubmed
Endothelial response to stress from exogenous
Zn2 + resembles that of
NO - mediated nitrosative stress , and is protected by
MT-1 overexpression . -
pubmed
Furthermore , we found
Zn ( 2 +)-exposed PAECs exhibited a significant reduction in mitochondrial membrane potential , loss of cardiolipin from the inner leaflet ,
caspase activation , and significant increases in TdT - mediated
dUTP nick end labeling - positive cells . -
pubmed
Furthermore , using an adenoviral construct for the overexpression of the
Zn ( 2 +)-binding protein ,
metallothionein-1 (
MT-1 ) , we found either
MT-1 overexpression or coincubation with a
Zn ( 2 +)-selective chelator ,
N , N , N ' , N '- tetrakis ( 2 - pyridylmethyl ) ethylene - diamide , in PAECs significantly protected the mitochondria from both
NO and
Zn ( 2 +)-mediated disruption and induction of apoptosis and cell
death . -
pubmed
In every case , we analyzed the plasma levels of
glucose ,
ferritin and other biochemical parameters . -
pubmed
The increased mitotic activity induced by CPG and
mGnRH was abolished by the addition of
mGnRH antagonists and anti -
mGnRH antibody , suggesting that the spermatogonial proliferation is regulated by
GnRH - like peptide in CPG of the scallop . -
pubmed
These findings suggest that the spermatogonial proliferation at phase
II in the scallop may be under the neuroendocrine control by
GnRH neuron in CPG . -
pubmed
In this study ,
horseradish peroxidase (
HRP)-labelled monoclonal anti -
LH can catalyse the
luminol -
hydrogen peroxide reaction . -
pubmed
Within 14 min , free
enzyme conjugate and immune complex could be separated in
alkaline borate buffer by means of a high voltage ( 15 kV ) . -
pubmed
One of the earliest events produced by this hormone is inhibition of the plasma membrane enzyme
adenylyl cyclase (
AC ) , with the concomitant drop in intracellular
cAMP levels and reinitiation of the cell cycle . -
pubmed
Overexpression of
xRic-8 , through mRNA injection , greatly inhibits
progesterone induced oocyte maturation and endogenous
xRic-8 mRNA depletion , through siRNA microinjection , induces spontaneous oocyte maturation . -
pubmed
On the other hand , the expression of
PvNADH - GOGAT - I , but not
PvNADH - GOGAT - II , was inhibited with
nitrogen compounds . -
pubmed
In silico analyses of the
NADH - GOGAT promoters revealed the presence of potential cis elements in them that could mediate differential tissue - specific , and
sugar and
amino acid responsive expression of these genes . -
pubmed
Phenylglycine and
phenylalanine derivatives as potent and selective
HDAC1 inhibitors ( SHI-1 ) . -
pubmed
An HTS screening campaign identified a series of low molecular weight
phenols that showed excellent selectivity ( & gt;100 - fold ) for
HDAC1 /
HDAC2 over other
Class I and Class II HDACs . -
pubmed
Two - dimensional temperature fields are measured in lean and sooting flames by means of two - color laser - induced fluorescence (
LIF ) imaging that uses seeded
NO . -
pubmed
Group 3 received
phosphate buffered saline solution (
PBS ) ICI and 2.5 x 10(11 ) vpu Ad beta - gal ICI , 10 days later . -
pubmed
Total and phosphorylated
Akt and endothelial
nitric oxide were analyzed by Western blot . -
pubmed
The
VEGF trap eliminated the beneficial effects of
bFGF on endothelium - dependent vasoreactivity and decreased
Akt and
nitric oxide phosphorylation . -
pubmed
This article provides an overview of the prevalence , aetiology , and mechanisms of
tissue injury arising as a result of
post -
stroke hyperglycaemia , as well as exploring the evidence from
glucose - lowering treatment trials to date . -
pubmed
Nitric oxide (
NO ) is a reactive molecule synthesized by
NO synthases (
NOS ) . -
pubmed
During the study the following were measured :
estradiol (
E2 ) ,
progesterone ( P4 ) ,
follicle - stimulating hormone (
FSH ) ,
luteinizing hormone (
LH ) , and histopathological study of the uteri , tubes , and ovaries . -
pubmed
In the present study , we show that truncated
Delta7 -
ERalpha attenuates WT -
ERalpha - driven gene expression across a wide range of
estrogen concentrations in cells that express functional
ERalpha at base line or upon co - transfection of full - length
ERalpha . -
pubmed
METHODS AND RESULTS : When HCAECs were treated with recombinant
cHSP60 ,
endothelial nitric oxide synthase (
eNOS ) mRNA and protein levels , enzyme activities , cellular
NO levels , mRNA stability , and promoter activities were significantly decreased . -
pubmed
Superoxide anion production was significantly increased due to the inhibition of mitochondrial membrane potential and
catalase and
superoxide dismutase (
SOD ) activities as well as activation of
NADPH oxidase . -
pubmed
Antioxidant
seleno - l - methionine (
SeMet ) or
SOD mimetic
MnTBAP effectively blocked
cHSP60 - induced
eNOS downregulation . -
pubmed
The parent drug and the potential metabolites were identified by two independent qualitative parameters , retention time (
tR ) and
MS /
MS spectrum . -
pubmed
Plateau potentials and persistent activity were suppressed by the generic nonselective cation channel blockers
FFA ( 100 muM ) and
2 - APB ( 100 muM ) , as well as by the
TRPC channel blocker
SKF-96365 ( 50 muM ) . -
pubmed
However , plateau potentials were not affected by the
TRPV channel blocker
ruthenium red ( 40 muM ) . -
pubmed
The
TRPC3 / 6 / 7 activator
OAG did not induce or enhance persistent firing evoked by
carbachol . -
pubmed
Voltage
clamp recordings revealed a
carbachol - activated , nonselective cationic current with a heteromeric TRPC - like phenotype . -
pubmed
Participants were randomized either to treatment as usual ( TAU ) or to TAU plus
CBT -
GSH . -
pubmed
RESULTS : Compared to those receiving
TAU only , those who received
TAU plus
CBT -
GSH experienced 25.2 more binge - free days and had lower total societal costs of $ 427 over 12 months following the intervention ( incremental
CEA ratio of -$20.23 per binge - free day or -$26,847 per QALY ) . -
pubmed
Lower costs in the
TAU plus
CBT -
GSH group were due to reduced use of
TAU services in that group , resulting in lower net costs for the
TAU plus
CBT group despite the additional cost of
CBT -
GSH . -
pubmed
CONCLUSIONS : Findings support
CBT -
GSH dissemination for recurrent binge - eating treatment . -
pubmed
This potentiating effect of
H ( 2 ) O ( 2 ) was blocked by the pretreatment with either 10,000 - unit / mL
catalase or 300 - microM
N - acetyl - cysteine . -
pubmed
The potentiating effect of
H ( 2 ) O ( 2 ) was occluded by an
adenylate cyclase activator ,
forskolin , and was blocked by a
protein kinase A inhibitor ,
N -( 2 -[ p - bromocinnamylamino ] ethyl )- 5 - isoquinolinesulfonamide hydrochloride . -
pubmed
This study indicates that oxidative stress may potentiate presynaptic
GABA release through the mechanism of
cAMP - dependent
protein kinase A (
PKA)-dependent pathways , which may result in the inhibition of the cerebral cortex neuronal activity . -
pubmed
Selective knockdown of
ceramide synthases reveals complex interregulation of
sphingolipid metabolism . -
pubmed
However , the contribution of each
CerS to steady - state levels of specific
Cer species has not been evaluated . -
pubmed
Additionally , each
CerS knockdown produced unique effects on the levels of multiple
sphingolipid species . -
pubmed
For example , downregulation of
CerS2 decreased very long - chain
Cer but increased levels of
CerS4 ,
CerS5 , and
CerS6 expression and upregulated long - chain and medium - long - chain
sphingolipids . -
pubmed
Conversely ,
CerS6 knockdown decreased
C16:0 - Cer but increased
CerS5 expression and caused non - C16:0
sphingolipids to be upregulated . -
pubmed
Knockdown of individual
CerS failed to decrease total
sphingolipids or upregulate
sphingoid bases . -
pubmed
Treatment with siRNAs targeting combined
CerS ,
CerS2 ,
CerS5 , and
CerS6 , did not change overall
Cer or
sphingomyelin mass but caused upregulation of
dihydroceramide and
hexosyl - ceramide and promoted endoplasmic reticulum stress . -
pubmed
These data suggest that
sphingolipid metabolism is robustly regulated by both redundancy in
CerS - mediated
Cer synthesis and counter - regulation of
CerS expression . -
pubmed
SGK3 is an
estrogen - inducible kinase promoting
estrogen - mediated survival of
breast cancer cells . -
pubmed
Here we report that
SGK3 is an
estrogen receptor (
ER ) transcriptional target and promotes
estrogen - mediated cell survival of
ER - positive breast cancer cells . -
pubmed
In
ER - positive breast cancer cells ,
SGK3 expression was found to be induced by
17beta - estradiol ( E(2 ) ) in a dose- and time - dependent manner , and the induction of
SGK3 mRNA by E(2 ) is independent of newly synthesized proteins . -
pubmed
Together , our study defines the molecular mechanism of regulation of
SGK3 by
estrogen / ER and provides a new link between the
PI3 K pathway and
ER signaling as well as a new
estrogen - mediated cell survival mechanism mediated by
SGK3 in
breast cancer cells . -
pubmed
The
DIF derivatives differentially affected cell differentiation and chemotaxis , suggesting the possible existence of at least three receptors for
DIFs : one receptor responsible for stalk cell induction , and two receptors responsible for chemotaxis modulation . -
pubmed
Furthermore , our results indicate that
DIF derivatives can be utilized to analyze the
DIF - signaling pathways . -
pubmed
RESULTS : High
glucose increased
MMP2 and decreased
connexin 43 in the mitochondria of retinal endothelial cells . -
pubmed
Blood pressure - dependent and independent effects of
amlodipine and
angiotensin receptor blockers on the risk of
stroke . -
pubmed
Both transport
purine and
pyrimidine nucleosides and are distinguished functionally by a difference in sensitivity to inhibition by nanomolar concentrations of
nitrobenzylmercaptopurine ribonucleoside (
NBMPR ) ,
hENT1 being
NBMPR - sensitive . -
pubmed
The same study also revealed lower , but significant , transport of
hypoxanthine by h /
rENT1 . -
pubmed
Fluxes of
hypoxanthine ,
thymine , and
adenine by
hENT1 were saturable and inhibited by
NBMPR . -
pubmed
Nucleobase transport activity was absent from an engineered
cysteine - less version
hENT1 (
hENT1C- ) in which all 10 endogenous
cysteine residues were mutated to
serine . -
pubmed
Site - directed mutagenesis identified
Cys-414 in transmembrane helix 10 of
hENT1 as the residue conferring
nucleobase transport activity to the wild - type transporter . -
pubmed
Substitution of a single
amino acid residue in the aromatic /
arginine selectivity filter alters the transport profiles of
tonoplast aquaporin homologs . -
pubmed
X - ray crystallography of
aquaporins indicates that four
amino acids constitute an aromatic /
arginine ( ar / R ) pore constriction known as the selectivity filter . -
pubmed
TgTIP1;1 and
TgTIP1;2 have a novel
isoleucine in loop E ( LE2 position ) of the ar / R filter ; the residue at LE2 is a
valine in all group I
TIPs from model plants . -
pubmed
TgTIP1;1- and
TgTIP1;2 - mediated
H ( 2 ) O ( 2 ) conductance was demonstrated further by a fluorescence assay . -
pubmed
In contrast , mutants that mimicked group
II TIPs showed no evidence of
urea or
H ( 2 ) O ( 2 ) conductance . -
pubmed
These results suggest that the
amino acid residue at LE2 position is critical for the transport selectivity of the
TIP homologs and
group I TIPs might have a broader spectrum of substrate selectivity than
group II TIPs . -
pubmed
METHODS : As
aspirin is released from prodrugs following
esterase hydrolysis we studied the expression and activity of
butyrylcholineterase (
BuChE ) and
carboxyesterase (
CE ) in plasma and platelets . -
pubmed
Finally , the relative contribution of
nitric oxide (
NO ) bioactivity to
nitrate - containing prodrugs of
aspirin - induced inhibition of aggregation was determined using
1H -[ 1,2,4 ] oxadiazolo [ 4,3 - a ] quinoxalin-1 - one (
ODQ , ) a selective inhibitor of the soluble
guanylyl cyclase . -
pubmed
KEY FINDINGS :
ST0702 , 2 , a
nicotinic acid -
aspirin codrug was equipotent with
aspirin with respect to inhibition of
collagen - induced
platelet aggregation . -
pubmed
The platelet inhibitory effects of
aspirin prodrugs were time - dependent as the maximal inhibitory effects against
collagen - induced aggregation were achieved by
aspirin at 2 min , 1 at 5 min and
ST0702 at 15 min . -
pubmed
The
aspirin prodrugs were significantly less potent in WP than in
PRP and the reverse was true of
aspirin . -
pubmed
In the presence of complete
BuChE inhibition in
PRP , there was almost complete loss of
aspirin prodrug , but not
aspirin anti - aggregatory activity . -
pubmed
Interestingly ,
CE activity was observed in WP and platelet lysate with
pNPA substrate . -
pubmed
Accordingly , 1 and
ST0702 retained 50 % and 100 % anti - aggregatory activity at maximal concentrations in WP , which was attenuated in the presence of
esterase inhibitor
phenylmethylsulphonyl fluoride . -
pubmed
CONCLUSIONS : The inhibitory effect of
aspirin prodrugs in
PRP is due to prodrug activation by
BuChE. -
pubmed
DC were matured for 24 h with
TNF - alpha and
prostaglandin E ( 2 ) (
PGE ( 2 ) ) . -
pubmed
The static respiration tests led to 2 - 2.5 times lower
oxygen - related indices compared to those calculated in dynamic respiration tests (
DRT ) ; the 7 - day
CO ( 2 ) cumulative generations were , however , almost similar for both types of tests . -
pubmed
AIM : To determine the prevalence and to identify the risk factors of
chloroquine maculopathy (
CM ) , and to evaluate the association of plasma
chloroquine ( CQ ) and
desethylchloroquine (
DCQ ) levels and
CM . -
pubmed
Synergistic enhancement of
cancer therapy using a combination of
heat shock protein targeted
HPMA copolymer - drug conjugates and gold nanorod induced
hyperthermia . -
pubmed
In this study ,
tumor hyperthermia was utilized as a means to increase the active delivery of
heat shock protein (
HSP ) targeted
N -( 2 - hydroxypropyl ) methacrylamide (
HPMA ) copolymer - drug conjugates . -
pubmed
Conjugates bearing the anticancer agents
aminohexylgeldanamycin (
AHGDM ) ,
docetaxel (
DOC ) , or
cisplatin and the
GRP78 targeting peptide WDLAWMFRLPVG were synthesized and characterized . -
pubmed
HSP targeted
AHGDM and
DOC conjugates demonstrated active binding comparable to native targeting peptide . -
pubmed
Based on these results ,
HSP targeted
DOC conjugates were selected for in vivo evaluation . -
pubmed
( Photochem Photobiol 10:309 - 329 , 1969 ) designed and employed an elegant and highly sensitive electrochemical technique capable of measuring
O2 evolved by
photosystem II (
PSII ) in response to trains of single turn - over light flashes . -
pubmed
The measurement and analysis of flash - induced
oxygen evolution patterns ( FIOPs ) has since proven to be a powerful method for probing the turnover efficiency of
PSII . -
pubmed
Cell cycle analysis ,
methyl - thiazol - diphenyltetrazolium assay ,
alkaline phosphatase activity , mineralization behavior , and gene expression of odontoblast phenotype
SCAP cultured with or without CM from PAFCs were evaluated . -
pubmed
Analysis of the assembled contigs via two different methods , produced 36 new pre - pro - peptides , coding for 61 neuropeptides or peptide hormones belonging to the following families : Allatostatins ( A , B et C ) ,
Bursicon ( alpha and beta ) , Crustacean Hyperglycemic Hormones (
CHH and
MIH /
VIHs ) ,
Crustacean Cardioactive Peptide (
CCAP ) ,
Corazonin ,
Diuretic Hormones ( DH ) , the
Eclosion Hormone (
EH ) ,
Neuroparsin ,
Neuropeptide F (
NPF ) ,
small Neuropeptide F (
sNPF ) ,
Pigment Dispersing Hormone (
PDH ) ,
Red Pigment Concentrating Hormone (
RPCH ) and finally
Tachykinin . -
pubmed
We also analyzed the transcripts of three
citrate channel proteins and found they were up - regulated by
NaCl ,
CuSO4 , and
AlCl3 but not by IM or DM . -
pubmed
APPROACH AND RESULTS : Platelets displayed all 3 primary
proteasome protease activities , which
MG132 and
bortezomib (
Velcade ) inhibited . -
pubmed
The effects of proteasome inhibitors were not confined to a single receptor as
MG132 suppressed
thrombin - stimulated ,
ADP - stimulated , and lipopolysaccharide - stimulated microparticle shedding . -
pubmed
Mass spectrometry revealed a single
MG132 - sensitive tryptic cleavage after R1745 in an extended
Filamin A loop , which would separate its
actin - binding domain from its
carboxy terminal glycoprotein Ibalpha - binding domain . -
pubmed
These findings show that
progesterone increases
n-3 PUFA biosynthesis by up - regulating the mRNA expression of genes involved in this pathway , possibly via changes in the epigenetic regulation of
FADS2 . -
pubmed
Here , we analysed the lipids in inflammatory exudates using liquid chromatography - tandem mass spectrometry and identified a novel
DHA metabolite ,
14,20 - dihydroxy - DHA (
14,20 - diHDHA ) and showed that it is biosynthesized by eosinophils through the
12 / 15 - lipoxygenase pathway . -
pubmed
White " x - ray radiation from the Advanced Photon Source was used to initiate the chemical decomposition of
C6F14 , which resulted in the in situ production of
F2 as verified via Raman spectroscopy . -
pubmed
Induction of
H2AX phosphorylation in
tumor cells by
gossypol acetic acid is mediated by
phosphatidylinositol 3 - kinase ( PI3 K ) family . -
pubmed
Thus , we further examined the possible mechanisms of
GAA to induce
gammaH2AX in
tumor cells . -
pubmed
However , it has been less well described for
icotinib , a similar orally small - molecule
tyrosine kinase inhibitor ( TKI ) . -
pubmed
Disulfide - bond scrambling promotes amorphous aggregates in
lysozyme and
bovine serum albumin . -
pubmed
In this study , we report the effect of
disulfide - reducing agent
dithiothreitol (
DTT ) on
hen egg white lysozyme (
lysozyme ) and
bovine serum albumin (
BSA ) aggregation at pH 7.2 and 37 C.
BSA and
lysozyme proteins treated with
disulfide - reducing agents form very distinct amorphous aggregates as observed by scanning electron microscope . -
pubmed
BSA and
lysozyme aggregates show unique but measurable differences in
8 - anilino-1 - naphthalenesulfonic acid (
ANS ) and
4,4 '- dianilino-1,1 '- binaphthyl-5,5 '- disulfonic acid (
bis - ANS ) fluorescence , suggesting a loose and flexible aggregate structure for
lysozyme but a more compact aggregate structure for
BSA . -
pubmed
Similar amorphous aggregates were also generated using a
nonthiol - based reducing agent ,
tris ( 2 - carboxyethyl ) phosphine (
TCEP ) , at pH 7.2 and 37 C. In summary , formation of distinct amorphous aggregates by
disulfide - reduced
BSA and
lysozyme suggests an alternate pathway for protein aggregation that may be relevant to several proteins . -
pubmed
Binding of different ligands like
GalNAc ,
lactose , and
galactose onto native and demetallized
protein was studied by isothermal titration calorimetry as well as molecular simulation methods . -
pubmed
Design , synthesis and biological evaluation of novel
pyrazoline - containing derivatives as potential
tubulin assembling inhibitors . -
pubmed
Total synthesis of a
biotinylated rocaglate : Selective targeting of the
translation factors eIF4AI / II . -
pubmed
Affinity purification revealed that
biotinylated rocaglate ( 8) and
methyl rocaglate ( 2 ) bind with high specificity to
translation factors eIF4AI / II . -
pubmed
RESULTS :
Thyroid - stimulating hormone ,
vitamin B12 and
insulin levels were significantly lower in the study group than controls ( p=0.001 , p=0.049 and p=0.033 , respectively ) . -
pubmed
All had a circumscribed central
maculopathy with borders that did not respect posterior
pole horizontal arterioles ( typically extending beyond the major arcades ) and associated with polygonal
pigment clumping . -
pubmed
To target extended - spectrum
beta - lactamase (
ESBL)-producing pathogens , novel
beta - lactam antibiotics , such as
ceftolozane /
tazobactam ,
ceftazidime /
avibactam ,
aztreonam /
avibactam ,
imipenem /
relebactam and
S-649266 , are antimicrobial alternatives for cIAIs . -
pubmed
The
FA and
AD values in the left posterior limb of internal capsule correlated negatively with the
heroin - positive urinalysis rate within follow - up . -
pubmed
These observations prompted the US FDA to revise statin labels to include a warning of an increased risk of incident
diabetes mellitus as a result of increases in
glycosylated hemoglobin (
HbA1c ) and fasting plasma
glucose . -
pubmed
RESULTS : Risk factors associated with
MRSA SSIs identified by the Delphi method were : patients from long - term care facilities , recent hospitalization ( within the preceding 30 days ) , Charlson score & gt ; 5 points ,
chronic obstructive pulmonary disease and thoracic surgery , antibiotic therapy with
beta - lactams ( especially
cephalosporins and
carbapenem ) and/or
quinolones in the preceding 30 days , age 75 years or older , current duration of hospitalization & gt;16 days , and surgery with prothesis implantation . -
pubmed
LY294002 induces differentiation and inhibits invasion of
glioblastoma cells by targeting
GSK-3beta and
MMP . -
pubmed
Taken together , these findings suggest differentiation - inducing and invasion - inhibitory effectiveness of
LY294002 in
glioblastomas , most likely involving inhibition of
GSK-3beta and
MMP respectively . -
pubmed
Low - Density Lipoprotein Cholesterol , Non - High - Density Lipoprotein
Cholesterol ,
Triglycerides , and
Apolipoprotein B and Cardiovascular Risk in Patients With
Manifest Arterial Disease . -
pubmed
Low - density lipoprotein cholesterol (
LDL - C ) only partly represents the atherogenic lipid burden , and a growing body of evidence suggests that non - high - density lipoprotein
cholesterol ( non - HDL - C ) ,
triglycerides , and
apolipoprotein B (
apoB ) are more accurate in estimating lipid - related
cardiovascular disease risk . -
pubmed
Adjusted hazard ratios ( HRs ) of MACE per 1 SD higher were for
LDL - C ( HR 1.15 , 95 % confidence interval [ CI ] 1.09 to 1.22 ) , for non -
HDL - C ( HR 1.17 , 95 % CI 1.11 to 1.23 ) , for log(
triglycerides ) ( HR 1.12 , 95 % CI 1.06 to 1.19 ) , and for
apoB HR ( 1.12 , 95 % CI 0.99 to 1.28 ) . -
pubmed
Transcription factors such as
NST1 may regulate genes involved in
phenylpropanoid pathway and affect development and lignification of the endocarp . -
pubmed
Moreover , a 3 year - old
Vitamin E /
HPbetaCD - IC NF sample has shown very similar antioxidant efficiency when compared with freshly prepared
Vitamin E /
HPbetaCD - IC NF indicating that long - term stability was achieved for
Vitamin E in the
CD - IC fiber matrix . -
pubmed
First simultaneous generation and utilization of both
copper - triazole and - ketenimine intermediates in
copper - catalyzed
sulfonyl azide - alkyne cycloaddition reactions is achieved for the one -
pot synthesis of
triazolopyrimidines via a novel
copper - catalyzed multicomponent cascade of
sulfonyl azides ,
alkynes , and
azirines . -
pubmed
Through the important women who lived their lives fighting against this disease and its complications , we would like to pay tribute to all those women who still
today , in an
era of antibiotics and where
syphilis is a treatable pathology , they can not be cured because they are both geographically and socially disadvantaged . -
pubmed
To check the applicability of JADE in real cases , JADE was used for resolution and quantification of
phenanthrene and
anthracene in aromatic fraction of heavy fuel oil ( HFO ) analyzed by GCxGC -
MS . -
pubmed
In addition , LOD values ( mug / mL ) were respectively 1.36 and 1.24 for
phenanthrene and 1.26 and 1.09 for
anthracene using MCR -
ALS and JADE which showed outperformance of JADE over MCR -
ALS . -
pubmed
A liquid chromatography - high resolution mass spectrometry ( LC - HRMS ) method was developed and validated for the determination of
piperine (
PPR ) on dried blood
spots (
DBS ) . -
pubmed
Results showed that
ETB receptor agonist ,
sarafotoxin 6c induced a negligible contraction in fresh artery segments , while
ETA receptor agonist ,
ET-1 induced an obvious contraction . -
pubmed
The
U0126 (
MEK /
ERK1 / 2 inhibitor ) and
SB203580 (
p38 inhibitor ) significantly attenuated both organ cultured - induced and
PM2.5 - induced up - regulation of
ETB receptor . -
pubmed
U0126 also suppressed organ culture - increased and PM2.5 - increased expressions of
ETA receptor . -
pubmed
SB203580 only suppressed PM2.5 - induced enhanced expressions of
ETA receptor In conclusion , airborne PM2.5 up - regulates
ETB and
ETA receptors of mesenteric artery via
p38 MAPK and
MEK /
ERK1 / 2 MAPK pathways . -
pubmed
Thyroid
H2O2 generation was increased in DM , which was reversed by
MSC treatment . -
pubmed
A strong peak between 2 and 4 keV showed the greatest ratio of the elemental
silver signals , due to surface plasmon resonance (
SPR ) . -
pubmed
Immunofluorescence , Polscope equipment and quantitative reverse transcription
polymerase chain reaction ( RT - qPCR ) were used to analyze damage , including mitochondrial distribution , reactive
oxygen species ( ROS ) levels , spindle morphology , and gene expression levels (
Bcl-2 ,
BAX , and
MnSOD ) . -
pubmed
RT - qPCR showed that the expression of
Bcl-2 was lower in the control group compared with the
GSH - OEt group ;
BAX and
MnSoD expression levels were higher in the control group than in the
GSH - OEt group ( p & lt ; 0.05 ) . -
pubmed
I - X ) ; 2 ) LC - accurate
MS /
MS and quantitative
1H NMR spectroscopy conducted in parallel to acquire the qualitative and quantitative data of each fraction ; 3 ) Robust identification and quantification of components by use of LC coupled to multiple reaction monitoring . -
pubmed
Using beta - CD as the particle core ,
polyethylene glycol (
PEG ) chains were conjugated to the primary
face via non - cleavable bonds and amphiphilic polymers incorporating hydrophobic and cationic monomers were grafted to the secondary
face via acid - labile linkages . -
pubmed
We evaluated the efficacy of
EPPT1 peptides presented on the free tips of the
PEG brush to function as a targeting ligand against underglycosylated
MUC1 (
uMUC1 ) receptors overexpressed on the surface of metastatic
breast cancer cells . -
pubmed
Overexpression of
RCK1 significantly improved
glucose and
xylose fermentation under
acetic acid stress conditions . -
pubmed
Specifically , the
RCK1 - overexpressing
strain exhibited a two - fold higher specific
ethanol productivity than the control
strain in
glucose fermentation under the presence of
acetic acid . -
pubmed
cerevisiae overexpressing
RCK1 showed 40 % lower intracellular reactive
oxygen species ( ROS ) levels as compared to the parental
strain when the
strains were exposed to
acetic acid , suggesting that
RCK1 overexpression might play a role in reducing the oxidative stress caused by
acetic acid . -
pubmed
A
Polyamidoamine Dendrimer - Based Electrochemical Immunosensor for Label - Free Determination of
Epithelial Cell Adhesion Molecule- Expressing Cancer Cells . -
pubmed
The
amino - terminated
polyamidoamine dendrimer (
G6 PAMAM ) was first covalently attached to the
3 - mercaptopropionic acid (
MPA)-functionalized gold electrode to obtain a thin film , and then completely carboxylated by
succinic anhydride (
SA ) . -
pubmed
Next , the Anti -
EpCAM was covalently bound with the
G6 PAMAM to obtain a stable recognition layer . -
pubmed
Notably , endothelial vasodilatation of mesenteric arteries was impaired in SHR adolescent offspring exposed to prenatal
hypoxia , vascular responses to
acetylcholine (
ACh ) and
sodium nitroprusside (
SNP ) were reduced , as well as plasma
nitric oxide levels and expression of
endothelial nitric oxide synthase (
eNOS ) in vessels were decreased . -
pubmed
Moreover , mesenteric arteries in SHR offspring following prenatal
hypoxia showed enhanced constriction responses to
phenylephrine (
PE ) , associated with up - regulated activities of
L - type calcium channel (
Ca2 + -dependent ) ,
RhoA /
Rock pathway signaling (
Ca2 + -sensitization ) , and intracellular
Ca2 + flow . -
pubmed
Furthermore , several proteins related to
calcium signaling pathway and pathogen related proteins were up - regulated , and we also found expression levels of
EDS1 ,
ICS1 , and
PR1 involved in
SA pathway up - regulated after
DNPE6 ( 4 ) treatment . -
pubmed
For
Mcl-1 , a
benzodioxane /
benzoxazine series was optimized to a K d of 40 muM before a
thienopyrimidine hit series was identified which subsequently led to the lead series from which the clinical candidate
S 64315 (
MIK 665 ) was identified . -
pubmed
For
Bcl-2 , the fragment - derived series were difficult to progress , and a compound derived from a published
tetrahydroquinone compound was taken forward as the hit from which the clinical candidate ( S 55746 ) was obtained . -
pubmed
Oral
glucose tolerance tests showed
glucose intolerance and decreased
insulin secretion in response to
glucose loading in affected males , features which were exacerbated with age . -
pubmed
Serum
alanine / aspartate aminotransferases (
ALT /
AST ) , liver
glutathione (
GSH ) depletion , as well as the inflammatory
cytokines , such as
tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1beta (
IL-1beta ) ,
inducible nitric oxide synthase (
iNOS ) , and
cyclooxygenase-2 (
COX-2 ) , were analyzed to indicate the underlying protective effects of
Rb1 against
APAP - induced
hepatotoxicity with significant inflammatory responses . -
pubmed
Importantly ,
Rb1 mitigated the changes in the phosphorylation of
MAPK and
PI3K /
Akt , as well as its downstream factor
NF - kappaB. -
pubmed
In conclusion , experimental data clearly demonstrated that
Rb1 exhibited a remarkable liver protective effect against
APAP - induced
ALI , partly through regulating
MAPK and
PI3K /
Akt signaling pathways - mediated inflammatory responses . -
pubmed
Therefore , our research aims to provide insights into the microstructure of
K - A - S - H particles synthesized with accurate stoichiometric control by the hydrothermal method through thermogravimetric analysis (
TG ) , Fourier transform infrared spectroscopy ( FTIR ) , nuclear magnetic resonance ( NMR ) , scanning electron microscopy ( SEM ) , transmission electron microscopy (
TEM ) and BET surface area . -
pubmed
The
TG results indicate that the crystalline phase contains more non - evaporated water or
zeolite water for structural rearrangement . -
pubmed
Importantly , TRP channel
TR structures have revealed sites and mechanisms of action of numerous synthetic and natural compounds , as well as those for endogenous ligands such as lipids ,
Ca2 + , and
calmodulin . -
pubmed
By contrast , under conditions of low
cortisol change , individuals low in
ERA outperformed those high in ERA . -
pubmed
Canagliflozin (
CANA ) , a
sodium - glucose cotransporter 2 (
SGLT2 ) inhibitor , affects various metabolisms . -
pubmed
For instance ,
CANA significantly downregulated
ATP synthase F1 subunit alpha , a mitochondrial electron transport system protein ( CON 297.28 + -20.63 vs. CANA 251.83 + -22.83 fmol/10 mug protein ; P = 0.0183 ) . -
pubmed
Moreover ,
CANA significantly downregulated
nucleoside diphosphate kinase 1 ( CON 110.30 + -11.37 vs. CANA 89.14 + -8.39 fmol/10 mug protein ; P = 0.0172 ) . -
pubmed
Melatonin inhibits the endoplasmic reticulum stress - induced ,
C / EBP homologous protein - mediated pathway in acute
pancreatitis . -
pubmed
However , the mechanisms of the anti - inflammatory effects of
melatonin ' are unknown , particularly the relationship between
melatonin and
ER stress . -
pubmed
Moreover , cells treated with
melatonin exhibited attenuated
inflammation , decreased expression levels of
ER stress - associated proteins and inhibition of apoptosis . -
pubmed
Results : A meta - analysis of the data extracted from three randomized control trials revealed that treatment with
exenatide yielded significant improvements in scores on the Unified
Parkinson ' s Disease Rating Scale Part I ( UPDRS - I ) ( -0.438 , 95 % CI , -0.828 to -0.048 , p = 0.028 ) , UPDRS Part
IV ( UPDRS -
IV ) ( -0.421 , 95 % CI , -0.811 to -0.032 , p = 0.034 ) and the Mattis Dementia Rating Scale ( MDRS ) ( -0.595 , 95 % CI , -1.038 to -0.151 , p = 0.009 ) . -
pubmed
More important ,
trelagliptin improved liver function by reducing
alanine transaminase ,
aspartate transaminase ,
lactate dehydrogenase , and total
bile acid . -
pubmed
Mechanistically , we found that the administration of
trelagliptin reduced the activity of hepatic
nuclear factor - kappaB but increased the activity of
AMP - activated protein kinase . -
pubmed
We hypothesized that repeated short - term exposures of
ammonia - oxidizing communities to free
ammonia (
FA ) and free
nitrous acid (
FNA ) would stabilize nitritation by selecting against
nitrite - oxidizing bacteria ( NOB ) . -
pubmed
A single stressor SBR (
SS / SBR ) using pH set - point control produced
HNO3 , while a dual stressor SBR (
DS / SBR ) using timed alkalinity addition (
TAA ) produced
HNO2 (
ammonium removal efficiency of 97 + - 2 % ;
nitrite accumulation ratio of 98 + - 1 % ) . -
pubmed
The diagnosis was stage
IV pancreatic cancer(unresectable ) , and the patient underwent chemotherapy with
GEM plus nab -
PTX . -
pubmed
This report examines the prevalence of
SHS exposure among nonsmoking U.S. adults in 2015 - 2018 based on blood levels of
cotinine , a metabolite of
nicotine . -
pubmed
In contrast , by adding
DBD after
PHA , RNA labelling was much more inhibited than DNA . -
pubmed
When
cyanocobalamin-57 Co was mixed with hog
intrinsic factor in water , excretion increased to 15.6 % + /- 1.6 % . -
pubmed
When
cyanocobalamin-57 Co and
intrinsic factor were given in two separate capsules , which is now a frequent practice , excretion was 9.5 % + /- 2.2 % . -
pubmed
When
cyanocobalamin-57 Co and
intrinsic factor are given in separate capsules , binding of radioactive vitamin to
intrinsic factor may be incomplete due to inadequate mixing in the stomach or to binding of
intrinsic factor to blocking antibody in the gastric juice . -
pubmed
The classic technique in which
intrinsic factor and
cyanocobalamin-57 Co are mixed in water before administration should be used . -
pubmed
Recently , a female hormone ,
progesterone , has been found to inhibit the expression of
alpha - fetoprotein in
hepatoma cells . -
pubmed
These results together with our hypothesis that the expression of
alpha - fetoprotein is regulated by
glucocorticoid receptor complex in proliferating
hepatocellular carcinoma cells lead to the conclusion that
steroid hormones and/or their antagonists may interfere with the function of
glucocorticoid receptors in
tumors , consequently regulate
tumor growth . -
pubmed
SslI activity was maximum at pH 8.8 , 0 to 50 mM
NaCl , 2 to 8 mM
Mg2 + , and 42 degrees C. Activity against phage DNA in vitro was demonstrated . -
pubmed
BMP is the only
molecule discovered thus far that is capable of inducing endochondral ossification in vivo . -
pubmed
Although chronic
ethanol treatment did not alter
2 - CADO- and
NaF - stimulated
adenylate cyclase activities in a cell - free preparation ,
2 - CADO- ,
forskolin , and
NaF - stimulated
adenylate cyclase activities were reduced when assays were carried out in
saponin - treated cells . -
pubmed
Chronic
ethanol exposure also increased the ability of in vitro
ethanol to stimulate
adenylate cyclase activity and
cyclic AMP content . -
pubmed
The increased sensitivity of
adenylate cyclase to in vitro
ethanol also was not observed when chronic
ethanol treatment was carried out with media containing delipidated serum . -
pubmed
Synergy between
zinc and
phorbol ester in translocation of
protein kinase C to cytoskeleton . -
pubmed
Treatment of cells for 40 min with a combination of
zinc ( 2 - 50 microM ) ,
zinc ionophore
pyrithione and unlabelled
phorbol dibutyrate ( 200 nM ) caused up to a ten - fold increase in cytoskeletal
protein kinase C and a corresponding decrease in other cellular compartments . -
pubmed
We propose that
zinc , like
calcium , regulates the distribution of
PKC in cells . -
pubmed
However , unlike
calcium which controls the binding of
PKC to the lipid component on cell membranes ,
zinc controls the distribution of
PKC to membrane cytoskeleton , possibly
actin . -
pubmed
[ The effect of
corticosteroid on
lymphokine - activated killer (
LAK ) activity and the change of membrane antigen phenotype on PBMC after
IL2 incubation with reference to the evaluation in vitro ] . -
pubmed
Moreover , the administration of
corticosteroid may reduce the toxicity of
IL2 . -
pubmed
In this context , the effect of
corticosteroid on
LAK activity and the change of membrane antigen phenotype on PBMC ( peripheral blood mononuclear cells ) was studied in vitro by using
methylprednisolone ( M -
PSL ) . -
pubmed
And the proliferation of PBMC after
IL2 incubation , which was measured by incorporation of
[ 3H ] TdR , was reduced dose - dependently by M - PSL . -
pubmed
These results suggest that the inhibitory effect of M - PSL on
LAK activation was caused not by preventing a triggering process of activation of precursor cells , but by a possible inhibition of proliferation , though other effects of
corticosteroid remain to be elucidated . -
pubmed
Like
N2O reductase ,
NosA contained
copper and was only made anaerobically . -
pubmed
In contrast to
N2O reductase , its synthesis was repressed by exogenous
copper ( but not by
Mn ,
Co ,
Ni ,
Zn , or
Fe ) . -
pubmed
To study whether or not
atrial natriuretic peptide (
ANP ) is physiologically involved in body fluid volume regulation , we examined the relationship between plasma
ANP level and renal function during
NaCl loading and
ANP infusion . -
pubmed
The plasma
ANP level increased by 60 % ( p less than 0.01 ) on the high
NaCl diet . -
pubmed
Although the plasma
ANP level was higher in HTs than in NTs , the changes in plasma
ANP due to
NaCl loading were similar between the two groups . -
pubmed
Furthermore , increases in urinary
Na excretion due to
ANP infusion at 25 ng / kg / min were greater for the high
NaCl diet than for the low
NaCl diet ( p less than 0.02 ) . -
pubmed
In study II , graded doses of
ANP were infusion into 16 other HTs and Nts on an 8 g / day
NaCl diet . -
pubmed
This rise in plasma
ANP was comparable to that induced by high
NaCl intake . -
pubmed
Thus , a slight increase in
plasma ANP induced by dietary
Na loading seems to augment renal
Na excretion , suggesting that
ANP may play a physiological role in body fluid volume regulation . -
pubmed
Densitometric
lecithin /
sphingomyelin ratios (
LSR ) and the presence of
phosphatidylglycerol (
PG ) were determined for 735 consecutively received amniotic fluids . -
pubmed
When the sample is introduced into the bioreactor ,
urea is first hydrolysed by
urease to produce
ammonia , which is then converted into
L - glutamate by
L - glutamate dehydrogenase . -
pubmed
L - Glutamate is finally oxidized by
L - glutamate oxidase to produce
hydrogen peroxide , which is quantified by measuring chemiluminescence emitted upon admixing with
luminol and
potassium ferricyanide . -
pubmed
Randomly selected patrons leaving taverns and bars were asked to participate in a study , and individuals agreed to answer a consumption questionnaire and have their blood
alcohol level (
BAL ) measured . -
pubmed
Acetone treatment did not alter the phospholipid and
cholesterol content of the microsomes , whereas
sodium phenobarbital ( positive control ) caused a significant decrease in
cholesterol content / mg microsomal
protein . -
pubmed
Proceedings : Effect of intravenous infusion of
oestradiol-17 beta , with and without prior
progesterone treatment , on the plasma
luteinizing hormone and
follicle - stimulating hormone concentrations in anoestrous ewes . -
pubmed
Analyses of such unusual microtubule arrays in
taxol - treated cultures may provide valuable insights into
tubulin - related systems in neurons , as well as in other cells . -
pubmed
The
deoxythymidine kinase (
dTK ) activity of a
5 - methoxymethyldeoxyuridine - resistant mutant ( MMdU(r)-20 ) of
herpes simplex virus type 1 was compared with that of the parental wild - type ( WT ) virus . -
pubmed
Further , it was inhibited to a lesser degree than the WT dTK by the
nucleoside analogs
MMdU and
arabinosylthymine (
araT ) , which suggests that one of the effects of the mutation was a selective alteration in substrate recognition by the dTK . -
pubmed
The loss of ability to inhibit the mutant dTK by
E -( 2 )- 5 - bromovinyldeoxyuridine was not as great as that seen with
araT and
MMdU. -
pubmed
[ 2 -( 14 ) C ] araT was used to explore further the resistance to
araT. -
pubmed
These findings suggest that a mutation in the
dTK polypeptide has affected recognition not only of
nucleoside substrates but of the
nucleotide substrate
dTMP as well , which agrees with the suggestion of Chen et al . -
pubmed
Light density marrow cells ( LMDCs ) were preincubated in Biorich ( chemically defined serum - free complete medium ) with
G + GM - CSF or
IL-3 prior to semi - solid culture with
Ara - C ( 10(-4 ) M-10(-12 ) M ) . -
pubmed
Data from 26 normal marrows studied clonogenically , demonstrated a 60 % reduction in CFU - GM with 10(-4 ) M
Ara - C. Preincubation in Biorich medium with the addition of
G + GM - CSF or
IL-3 caused a significant enhancement of sensitivity to
Ara - C across the dose curve . -
pubmed
Biorich medium alone had an apparent protective effect resulting in amelioration of the
cytotoxic effect of
Ara - C. Cell cycle analysis of eight subjects showed significant recruitment into S - phase following pre - treatment with
cytokines . -
pubmed
In summary , it would appear that
cytokines can enhance the sensitivity of normal CFU - GM to
Ara - C in vitro possibly due to an increase in S - phase activity . -
pubmed
( + /-)- 1 -( Cyclohexyloxycarbonyloxy ) ethyl 2 - ethoxy-1 -[[ 2 '-( 1H - tetrazol-5 - yl ) biphenyl-4 - yl ] methyl ]- 1H - benzimidazole-7 - carboxylate (
TCV-116 ,
Candesartan ) and its active metabolite
2 - ethoxy-1 -[[ 2 '-( 1H - tetrazol-5 - yl ) biphenyl-4 - yl ] methyl ]- 1H - benzimidazole-7 - carboxylic acid (
CV-11974 ) are specific nonpeptide
angiotensin AT1 receptor antagonists . -
pubmed
AIF4- inhibits influx of
Ca2 + into vascular smooth muscle cells . -
pubmed
The
cytoplasmic domain of
Ig beta is sufficient to induce these early
antigen - independent events by a mechanism that requires conserved
tyrosine residues in this protein . -
pubmed
Immunocompetition studies showed that import of precursors to
Rieske FeS protein ,
ATPase su9 -
DHFR , and the
adenine nucleotide transporter was inhibited in the presence of antibody to
MOM42 . -
pubmed
The
neurotensin antagonist
[ D - Trp11 ]- neurotensin ( 3 pmol ) was previously found to enhance
morphine , but not
beta - endorphin antinociception from the periaqueductal gray (
PAG ) when injected into the RMg . -
pubmed
A similar enhancement of
morphine , but not
beta - endorphin antinociception from the
PAG was observed in the current study by
[ D - Trp11 ]- neurotensin injections into the bilateral LPGi , bilateral Gi , or medial Gi . -
pubmed
These data suggest that neurotensinergic projections from the
PAG function in an antianalgesic manner throughout the RVM during
morphine , but not
beta - endorphin antinociception . -
pubmed
Hypotonic stress ( HTS ) activates a
Cl- current and releases intracellular
Ca2 + in vascular endothelial cells , but there is no co - activation of
K (+)- channels . -
pubmed
Affinity purification of the 1 %
Triton X-100 soluble stromal membrane fraction , on anti -
ubiquitin - sepharose revealed a ubiquitinated 55 kD subunit . -
pubmed
Using
potassium hydroxide (
KOH ) and
collagenase , the extracellular materials were successfully removed to expose the surface of muscle fibers . -
pubmed
The other tested compounds :
lysophospholipids ,
platelet - activating factor (
PAF ) and
GM1 - ganglioside were without effect on
A1 - AR expression . -
pubmed
The inhibition with
unsaturated FA was non - competitive and partially reversed by
albumin . -
pubmed
The
A1 - AR agonist binding inhibition evoked by
unsaturated FA in many respects is similar to that observed previously following
ischaemia and may be contributory to the increased excitability of
post - ischaemic brain . -
pubmed
In order to investigate the relation between the conformation and the ability to modulate
dopamine receptors , conformational free - energy calculations using an empirical potential ( ECEPP/3 ) and hydration shell model were carried out on the
tripeptide +
H2 - Pro - Leu - Gly - NH2 (
PLG ) and its analogues in which the
Leu residue is replaced with
Phe ,
Ahx ( L-2 - aminohexanoic acid ) ,
Ile ,
Abu and
Ala residues in the unhydrated and hydrated states . -
pubmed
PLG and two tripeptides possessing
Phe and
Ahx residues show
dopamine receptor modulating activity , while the other tripeptides do not . -
pubmed
Irrespective of activities ,
PLG and its analogues are found to have the high probabilities to form beta -
bends of types
II and I in the unhydrated state . -
pubmed
A key player in the lambda DNA packaging process is the phage - encoded enzyme
terminase , which is involved in ( i ) recognition of the concatemeric lambda DNA ; ( ii ) initiation of packaging , which includes the introduction of staggered nicks at
cosN to generate the cohesive ends of virion DNA and the binding of the prohead ; ( iii ) DNA packaging , possibly including the
ATP - driven DNA translocation ; and ( iv ) following translocation , the cutting of the terminal
cosN to complete DNA packaging . -
pubmed
The mechanism by which
CPT induces
cytotoxicity is the topic of continued research , but appears to be mediated by the stabilisation of transient " cleavable "
topoisomerase I - DNA complexes . -
pubmed
Furthermore , endothelial cell adhesion and spreading on
type IV collagen stimulates the secondary signaling events of
tyrosine phosphorylation of
focal adhesion kinase (
pp125FAK ) and autophosphorylation of
pp125FAK . -
pubmed
CAI treatment of the endothelial cells inhibited cell spreading , and both the induction of
pp125FAK phosphorylation and the phosphorylation of exogenous substrates by
pp125FAK kinase . -
pubmed
A model of the
BR96 antibody variable regions is compared to two X - ray structures of a
BR96 -
carbohydrate complex , independently determined after the model was built and analyzed . -
pubmed
Because
ADR resistance in these cells is associated with the expression of high levels of
P - glycoprotein (
Pgp ) , we evaluated the effect of anti -
estrogens on
Pgp expression and activity . -
pubmed
Both
ICI 182,780 and
TAM failed to modulate
Pgp expression as assessed by flow cytometry and Western - blot analysis , performed using the monoclonal antibodies MM4.17 and C 219 , which are specific for an external or an internal determinant respectively . -
pubmed
Pgp activity was investigated by flow cytometry measuring the extrusion of
ADR and the cationic dye
Rhodamine 123 (
Rh 123 ) . -
pubmed
Considerable physiological and anatomical evidence indicates that circulating
angiotensin II (
AngII ) , plays important roles in the long - term regulation of autonomic output as a result of actions in two circumventricular structures , the subfornical organ ( SFO ) and area postrema ( AP ) . -
pubmed
Through these actions circulating AngII is thus able to influence efferent projections from these CVO which in turn influence the output of hypothalamic cells projecting to the posterior pituitary (
vasopressin secretion ) , nucleus tractus solitarius (
NTS ) , and intermediolateral cell column of the spinal cord ( to influence sympathetic preganglionics ) , and medullary neurons in the
NTS . -
pubmed
We report a modified ion - pair extraction of interfering substances by
tetrahexylammonium chloride (
THAC ) in
ethyl acetate in the presence of
albumin , and a microtitre plate assay for biliary
beta - glucuronidase activity in bile with the substrate
p - nitrophenol - glucuronide . -
pubmed
Adding
albumin improved the recovery of
beta - glucuronidase activity to 99.8 % ( CV 1.9 % ) , and 92.2 % of the
bilirubin in bile samples was extracted in one step . -
pubmed
These results implicate activated
calpain II in
neurofibrillary degeneration , provide further evidence for the involvement of the calpain system in
AD pathogenesis , and imply that neuronal
calcium homeostasis is altered in
AD . -
pubmed
Leachate and soil samples collected from different tillage systems were analyzed for
atrazine using gas chromatography (
GC ) and an enzyme - linked immunosorbent assay ( ELISA ) based on magnetic particle technology . -
pubmed
Solid - phase extraction ( SPE ) was used to concentrate
atrazine residues in leachate samples and soil extracts before
GC analysis . -
pubmed
Atrazine concentrations determined by
GC ranged from 0.1 to 600 micrograms L-1 for water samples and from 1.0 to 700 micrograms kg-1 for soil samples . -
pubmed
Atrazine concentrations in 92 leachate samples as determined by ELISA were well - correlated ( R = 0.97 ) with
GC levels over the entire concentration range . -
pubmed
Where detection methods ( Method 1 versus Method 2 ) were compared on specific extracts ( & lt ; 10 %
MeOH ) , good agreement ( R = 0.99 ) was achieved between ELISA and
GC values , illustrating that control of extractant concentration is critical in using this assay for
atrazine detection in soil . -
pubmed
The role of
thiamin - dependent enzymes like
transketolase and the importance of the
NMDA (
N - Methyl - D - Aspartate ) System is discussed . -
pubmed
The synthesis of a variety of novel
4 - amido , 4 - carbamoyl and
4 - carboxamido derivatives of
1 -( 8 - chloro-6,11 - dihydro-5H - benzo [ 5,6 ] cyclohepta [ 1,2 - b ] pyridin-11 - yl ) piperazine to explore the
SAR of this series of
FPT inhibitors is described . -
pubmed
The synthesis of the corresponding
3 - bromo analogues resulted in the discovery of the
4 - pyridylacetyl N - oxides 83b ( + /- ) and 85b [ 11S(- ) ] and the
4 - carboxamidopiperidinylacetamido derivative 160b ( + /- ) , all of which exhibited potent
FPT inhibition in vitro . -
pubmed
Expression of the
receptor tyrosine kinase Ret on the plasma membrane is dependent on
calcium . -
pubmed
Here we show that expression of
p170(
ret ) is dependent on
calcium . -
pubmed
Depletion of extracellular
calcium completely blocks
p170 ( ret ) expression , which is not caused by a decrease in half - life of
p170 ( ret ) at the plasma membrane but by a defect in processing of
p150 ( ret ) into
p170 ( ret ) . -
pubmed
We propose that a relatively high concentration of luminal
calcium is necessary for the proper folding of
Ret in the endoplasmic reticulum . -
pubmed
The 4.5 - kb
HIAP2 message is contained within eight coding region exons and a single 5'UTR exon . -
pubmed
PSD-95 and
SAP102 mRNAs were prominently expressed at embryonic day 13 ( E13 ) in the mantle zone of various brain regions , where
NMDA receptor
NR2B subunit mRNA is expressed at high levels . -
pubmed
The
S11 cDNA encodes a 376
amino acid protein with a pI of 5.6 and a molecular mass of 42.9 kDa . -
pubmed
MTA (
LY231514 , multitargeted
antifolate ) represents a new class of
folate antimetabolites and inhibits multiple enzymes in the
purine and
thymidine biosynthetic pathways , including
thymidylate synthase ,
dihydrofolate reductase , and
glycinamide ribonucleotide formyl transferase . -
pubmed
The recently developed radioligand
[ 123I ] FP - CIT is suitable for clinical single - photon emission tomography ( SPET ) imaging of the
dopamine ( DA ) transporter in vivo . -
pubmed
In vivo
[ 123I ] FP - CIT binding to the
DA and serotonin transporters was evaluated after subchronic and acute administration of the
drugs . -
pubmed
The results of this study suggest that dopaminergic medication will not affect the results of
DA transporter SPET imaging with
[ 123I ] FP - CIT . -
pubmed
Among the antagonists for putative
melatonin receptors studied , neither
luzindole (
melatonin MT1 and MT2 receptor antagonist ) nor
prazosin (
melatonin MT3 receptor antagonist ) antagonized the
melatonin reversal of
morphine tolerance and dependence . -
pubmed
Overall , the present observations suggest that the
melatonin - induced reversal of
morphine tolerance and dependence may involve its ability to suppress
nitric oxide synthase (
NOS ) activity . -
pubmed
Further , the
melatonin - induced reversal of
morphine tolerance and dependence is not mediated through its actions via putative
melatonin receptors . -
pubmed
The agonistic activity of
melatonin towards peripheral
benzodiazepine receptors may partially contribute to the suppression of
morphine dependence but not to the reversal of tolerance to the analgesic activity of
morphine . -
pubmed
In contrast ,
Kv4.3 potassium channel alpha subunit mRNA was expressed at equal levels across the ventricular wall . -
pubmed
The
ShuS protein has no sequence homology with any proteins of known function and its role in
heme acquisition has not been determined . -
pubmed
It appears , therefore , that
ShuS may function as both a
heme storage protein , during periods of active
heme transport , and as a DNA binding protein to protect the DNA from any ensuing
heme mediated oxidative damage . -
pubmed
Although there are numerous mechanisms involved in the process of
renal disease progression , the
renin -
angiotensin system plays a major role as determined by the beneficial response to
angiotensin I - converting enzyme inhibitors ( ACEIs ) and
angiotensin II receptor blockers (
AT II blockers ) of reduced rate of progression in a variety of clinical and experimental
renal diseases . -
pubmed
Dual role of
Fyn in the regulation of
FAK + 6,7 by
cannabinoids in hippocampus . -
pubmed
In hippocampus endocannabinoids modulate synaptic function and plasticity and increase
tyrosine phosphorylation of several proteins , including
focal adhesion kinase (
FAK ) . -
pubmed
Autophosphorylation of
FAK on
Tyr-397 is generally a critical step for its activation , allowing the recruitment of
Src family kinases , and phosphorylation of
FAK and associated proteins . -
pubmed
Anandamide and
2 - arachidonoylglycerol , two
endocannabinoids , and
Delta9 - tetrahydrocannabinol , stimulated
tyrosine phosphorylation of
FAK + 6,7 , a neuronal splice isoform of
FAK , on several residues including
Tyr-397 . -
pubmed
These effects were sensitive to manipulation of
cAMP - dependent protein kinase , suggesting that they were mediated by inhibition of a
cAMP pathway . -
pubmed
PP2 , an
Src family kinase inhibitor , prevented the effects of cannabinoids on
p130 - Cas and on
FAK+6,7
tyrosines 577 and 925 , but not 397 , indicating that
FAK autophosphorylation was upstream of
Src family kinases in response to
CB1 - R stimulation . -
pubmed
These findings suggested that the
MMP inhibitor ,
R-94138 , could be used in adjunctive therapy for lymph node
metastasis in
gastric carcinoma . -
pubmed
In contrast , only a limited number of inhibitors of
KA receptor activity have been developed , most of which contain
quinoxalinedione or
decahydroisoquinoline skeletons . -
pubmed
The purpose of this study was to examine the effects of fluid replacement on power output ( PO ) , rating of perceived exertion (
RPE ) , heart rate (
HR ) ,
body weight ( BW ) , urine osmolarity ( Uosm ) , and urine
electrolyte concentrations ( [ UNa+ ] , [ UK+ ] ,
[ UCl - ] ) in physically active men ( n = 4 ) and women ( n = 7 ) . -
pubmed
The enzyme
tRNA - guanine transglycosylase (
TGT ,
EC 2.4.2.29 ) catalyzes a posttranscriptional transglycosylation reaction involved in the incorporation of the modified base
queuine [ Q , 7 -( 4,5 - cis - dihydroxy-2 - cyclopenten-1 - ylaminomethyl )- 7 - deazaguanine ] into tRNA . -
pubmed
coli
TGT (
alanine ,
asparagine ,
cysteine , and
glutamate ) . -
pubmed
All four mutant
TGTs were able to noncovalently bind
tRNA , but only the
glutamate mutant was able to form a stable complex with the RNA substrate under denaturing conditions that was comparable to wild type . -
pubmed
Furthermore , the
glutamate mutant was the only mutant
TGT that demonstrated significant activity . -
pubmed
Kinetic parameters were determined for this
enzyme and shown to be comparable to wild type , revealing that the
enzyme is considerably tolerant of the positioning of the
carboxylate . -
pubmed
Under conditions of high
enzyme concentrations and long time courses , the
alanine ,
asparagine , and
cysteine mutants showed very low levels ( ca . -
pubmed
INTRODUCTION : The main purpose of this contribution is to set
today 's situation of technology assessment and point to some organizing and integrative ways with the evolution concern
ASL ( Local Health Firm ) . -
pubmed
The aim of the work was to describe the actual health status and the extent and seriousness of the affliction before the substitution therapy with
opioid receptors agonist --
methadone . -
pubmed
In mechanical mixtures of
H - ZSM-5 and
In2O3 thermal auto - reductive solid - state ion exchange (
AR - SSIE ) was found to proceed upon treatment in high vacuum at 840 K resulting in the incorporation of
In+ ions into , and in an increase of the thermal stability of , the zeolitic component . -
pubmed
Parallel solution- and solid - phase synthesis of
spiropyrrolo - pyrroles as novel
neurokinin receptor ligands . -
pubmed
The combination of a
3,5 - bis ( trifluoromethyl ) phenyl needle with the
spiropyrrolo - pyrrole motive as a privileged
GPCR scaffold was the basis for designing a focused combinatorial library targeted towards the
neurokinin-1 receptor . -
pubmed
A tensile
strain applied to the substrate for 15 min significantly increased microtubule ( MT ) assembly by 32 + /- 7 % , with no apparent effect on the cells ' focal adhesions as revealed by immunofluorescence and quantitative analysis of
Triton X-100 - insoluble
vinculin levels . -
pubmed
Disruption of the MT network by prolonged treatments with low doses of either
nocodazole or
paclitaxel before the application of
strain abolished the redistribution of
RhoA and
Rac in response to the applied forces . -
pubmed
We also recorded non -
sodium currents from Purkinje cells of both genotypes to test whether the
Scn8a mutation induced changes in other ion channels . -
pubmed
Hepatic glycogen was assessed by natural abundance
13C nuclear magnetic resonance spectroscopy (
MRS ) . -
pubmed
After an oral dosing , blood samples were collected for a period of 60 h. Plasma concentrations of
haloperidol were determined using a liquid chromatographic electrospray mass spectrometric ( LC -
MS ) method . -
pubmed
Several proteins with diverse functions outside primary
carbon metabolism , such as the
isomerase ROC4 ,
lipoxygenase 2 involved in
jasmonic acid biosynthesis , and a
carbonic anhydrase (
CA1 ) , were surprisingly abundant in the range of 0.75 - 1.5 % of the total stromal mass . -
pubmed
Two - dimensional difference gel electrophoresis ( DIGE ) in combination with univariate ( Student 's t - test ) and multivariate data analysis , principal component analysis ( PCA ) and partial least squares discriminant analysis ( PLS - DA ) were used to study the anti - inflammatory effects of the
beta ( 2 )- adrenergic receptor (
beta ( 2 )- AR ) agonist
zilpaterol . -
pubmed
This inhibition was counteracted by addition of the
beta ( 2 )- AR antagonist
propranolol (
LZP ) . -
pubmed
The inhibition of
MIP-1beta by
zilpaterol and the involvement of the
beta ( 2 )- AR and
cAMP were confirmed using a specific immunoassay . -
pubmed
Different analyses were made : light microscopy , scanning electron microscopy ( SEM ) and energy dispersive spectroscopy ( EDS ) ,
calcium incorporation to newly formed tissue (
alizarin red ) , bone nodule formation ( von Kossa ) , fat aggregate formation (
oil red O ) ,
collagen type I immunofluorescence , DNA concentrations ,
alkaline phosphatase (
ALP ) activity , and
osteocalcin concentrations . -
pubmed
Formation of
MT1 /
MT2 heterodimers remains to be shown in native tissues but is suggested by the documented co - expression of
MT1 and
MT2 in many
melatonin - sensitive tissues , such as the hypothalamic suprachiasmatic nuclei , retina , arteries , and adipose tissue . -
pubmed
Interaction between
tyrosineZ and substrate water in active
photosystem II . -
pubmed
However , there is no direct evidence for the interaction between
Tyr(Z ) and substrate water in active
Photosystem II (
PSII ) , instead most experiments have been performed on inhibited
PSII . -
pubmed
EPR measurements observed that the maximum yield of
Tyr(Z ) oxidation at cryogenic temperature in the S(0 ) and S(1 ) states was around neutral pH and was essentially pH - independent . -
pubmed
Regular
insulin secretory oscillations despite impaired
ATP synthesis in
Friedreich Ataxia patients . -
pubmed
Basal secretion of
insulin occurs in an oscillating manner presumably triggered by
ATP - dependent feedback inhibition of glycolytic flux . -
pubmed
In the present study
Friedreich Ataxia patients with a presumptive impairment of
ATP synthesis in pancreatic beta - cells were evaluated for regularity of basal secretory oscillations of
insulin . -
pubmed
Aspirin can reduce
thrombin generation with the subsequent attenuation of
thrombin - mediated coagulant reactions such as
factor XIII activation . -
pubmed
Aspirin also acetylates
lysine residues in
fibrinogen resulting in increased
fibrin clot permeability and enhanced clot lysis as well as directly promoting fibrinolysis with high - dose
aspirin . -
pubmed
The variable effectiveness of
aspirin in terms of clinical outcomes and laboratory findings , which has been termed
aspirin resistance , may be related to these additional antithrombotic effects that are altered when associated with common genetic polymorphisms such as the Leu33Pro
beta ( 3 )- integrin or Val34Leu
factor XIII mutations . -
pubmed
Using Western blotting ,
PhIP was shown to induce expression of the DNA damage response proteins
p53 and
p21(
WAF1 /
CIP1 ) , and to inhibit cell growth while activating
G1 cell cycle checkpoint , a consequence of
PhIP - induced DNA damage . -
pubmed
For the genomic pathway ,
progesterone interacts with the
type - B nuclear progesterone receptor (
nPR - B ) , which decreases expression of contraction - associated genes . -
pubmed
For the nongenomic pathway ,
progesterone interacts with membrane - associated
PRs (
mPRs ) to modulate directly intracellular
Ca2 + and
cyclic adenosine monophosphate (
cAMP ) levels . -
pubmed
,
PR - A and/or
PR - C ) and changes in
nPR coregulator levels that together repress the transcriptional activity of
PR - B ; and ( 2 ) increased expression of specific
mPRs , especially
mPRss , which augments contractility by decreasing intracellular
cAMP . -
pubmed
Functional
progesterone withdrawal may be induced by locally produced
prostaglandins that alter myometrial
nPR expression . -
pubmed
( 3 )
As ( 2 ) O ( 3 ) could significantly down - regulate
DNA methyltransferase 1 (
DNMT 1 ) ,
DNMT3A and
DNMT3B gene at mRNA level in a dose - dependent manner . -
pubmed
Despite treatment with systemic
steroids , immunosuppressants , chemotherapy and
interferon , progressive deterioration occurred . -
pubmed
The effect of several metabolic genotypes on adduct levels were also investigated and higher
PhIP and
ABP adducts were associated with the rapid
NAT2 and/or rapid
NAT1 genotypes . -
pubmed
In the
allopurinol group , serum
uric acid levels ,
GFR , systolic and diastolic blood pressure , and
C - reactive protein (
CRP ) levels significantly improved ( P & lt ; 0.05 ) . -
pubmed
No correlation was observed between changes in
GFR and changes in
CRP , or blood pressure in the
allopurinol group . -
pubmed
FDG - PET /
CT finding of high uptake in
pulmonary alveolar microlithiasis . -
pubmed
Combined positron emission tomography and computed tomography using
18F - FDG (
FDG - PET /
CT ) showed the abnormal accumulation of
FDG in both lungs with a maximal standardized uptake value of 7.3 . -
pubmed
A morphological study of 200
atherosclerotic carotid sinus ( CS ) plaques obtained at carotid endarterectomy revealed the structural components and processes characteristic for severe
atherosclerosis ( foci of
atheromatosis and
edema ,
necrosis of
collagen and elastic fiber , newly formed vessels and
hemorrhages of various duration , lipophages and lymphocytes , portions of
fibrosis and
calcification , covering thinning and
ulceration ,
thrombi , and the contents of
plagues ( atheromatous masses ,
cholesterol crystals , lipophages ,
calcificates ) on their surface ) . -
pubmed
One -
pot synthesis of
carbazoles by
palladium - catalyzed N - arylation and oxidative coupling . -
pubmed
One -
pot N - arylation and oxidative coupling can be promoted by a common
palladium catalyst in the presence of appropriate additives :
palladium - catalyzed N - arylation of
anilines with
aryl triflates under the standard conditions followed by addition of
acetic acid under
oxygen or air atmosphere afforded various types of functionalized
carbazoles in good to excellent yields . -
pubmed
Evidence of Maillard reactivity was suggested using colorimetry ,
o - phthaldialdehyde assays , and
sodium dodecyl sulfate PAGE followed by
glycoprotein staining . -
pubmed
Resultant
glycoprotein dispersions exhibited altered functionality , in which case steady shear and small amplitude oscillatory rheology parameters were shown to be dependent on the specific reducing
sugar present . -
pubmed
The decrease of pH in the anaerobic self - fermentation of sterilized sludge was very lower ( from 6.81 to 6.56 ) because
NH ( 4 )(+ ) produced by degradation of
proteins could neutralize organic acids produced in the process . -
pubmed
A new
PEG-1000 - based dicationic ionic liquid exhibiting temperature - dependent phase behavior with
toluene and its application in one -
pot synthesis of
benzopyrans . -
pubmed
A new recyclable temperature - dependant phase - separation system comprised of
PEG-1000 - based dicationic acidic ionic liquid and
toluene was developed and used in one -
pot three - component condensation to prepare
benzopyrans in excellent yields ( up to 93 % ) . -
pubmed
Our data provide mechanistic evidence for the first time showing that
DIM potentiates the killing of
pancreatic cancer cells by down - regulation of constitutive as well as drug - induced activation of
NF - kappaB and its downstream genes (
Bcl - xL ,
XIAP ,
cIAP , and
survivin ) . -
pubmed
This was accompanied by loss of
phospho -
p65 and down - regulation of
NF - kappaB activity and its downstream genes (
Bcl - xL ,
survivin , and
XIAP ) , which correlated with reduced cell proliferation ( as assessed by
Ki-67 immunostaining of
tumor specimens ) and evidence of apoptosis [ as assessed by
poly ( ADP - ribose ) polymerase cleavage and terminal deoxynucleotidyl transferase - mediated
dUTP nick end labeling staining ] . -
pubmed
These results provide strong in vivo evidence in support of our hypothesis that
DIM could abrogate chemotherapeutic drug (
cisplatin ,
gemcitabine , and/or
oxaliplatin)-induced activation of
NF - kappaB , resulting in the chemosensitization of
pancreatic tumors to conventional therapeutics . -
pubmed
Taken together , these findings strengthen the argument that besides
mTOR - inhibitors , such as
rapamycin analogues ,
p27 and
CDKs could also be considered targets for
hamartoma therapeutics in
tuberous sclerosis . -
pubmed
Complex 1 displays
bright orange luminescence in
dichloromethane solution , arising from
pi ( phenylacetylide )--& gt;
pi *( Me ( 3 ) SiC [ triple bond ] CbpyC [ triple bond ] CSiMe ( 3 ) ) ( 3 ) LLCT and d(Pt)-->
pi *( Me ( 3 ) SiC [ triple bond ] CbpyC [ triple bond ] CSiMe ( 3 ) ) ( 3 )MLCT triplet states which are supported by DFT calculation . -
pubmed
There is a need to develop oral models of significant
liver injury and to evaluate the possible role of
CYP2E1 in
ethanol actions in such models . -
pubmed
These results show that
CYP2E1 plays a major role in
ethanol - induced
fatty liver and oxidant stress . -
pubmed
Then clinical symptoms , and levels of
body mass index (
BMI ) ,
obesity degree ,
alanine aminotransferase (
ALT ) ,
aspartate aminotransferase (
AST ) ,
gamma - glutamyl transpeptidase (
gamma - GT ) , total
cholesterol (
TC ) ,
triglyceride (
TG ) and
CT ratio of liver and spleen before and after treatment were compared between two groups . -
pubmed
This process is initiated by the recognition of the
Tyr- and
di - Leu - based endocytic motifs of
CFTR by the
AP-2 adaptor complex , leading to the formation of
clathrin - coated vesicles and the channel delivery to sorting / recycling endosomes . -
pubmed
Antioxidant indicators (
CAT ,
MDA and T - AOC ) and neurotransmitters (
NO ,
Glu and
Ach ) in cerebra were measured . -
pubmed
CAT activities ,
MDA ,
NO and
Glu contents were decreased in SIF H group only . -
pubmed
Tyrosine phosphorylation of the
Galpha - interacting protein GIV promotes activation of
phosphoinositide 3 - kinase during cell migration . -
pubmed
We demonstrate that
GIV is a
tyrosine phosphoprotein that directly binds to and activates
phosphoinositide 3 - kinase (
PI3 K ) . -
pubmed
Upon ligand stimulation of various receptors ,
GIV was phosphorylated at
tyrosine-1764 and
tyrosine-1798 by both receptor and
non - receptor tyrosine kinases . -
pubmed
These phosphorylation events enabled direct binding of
GIV to the
amino- and
carboxyl - terminal
Src homology 2 domains of
p85alpha , a regulatory subunit of
PI3 K ; stabilized receptor association with
PI3 K ; and enhanced
PI3 K activity at the plasma membrane to trigger cell migration . -
pubmed
Tyrosine phosphorylation of
GIV and its association with
p85alpha increased during metastatic progression of a
breast carcinoma . -
pubmed
These results suggest a mechanism by which multiple receptors activate
PI3 K through
tyrosine phosphorylation of
GIV , thereby making the
GIV -
PI3 K interaction a potential therapeutic target within the
PI3K -
Akt pathway . -
pubmed
This nanoparticle - dODN bioconjugate (
gammaFe ( 2 ) O ( 3 )@ dODN ) was designed to target the
signal transducer and activator of transcription 3 ,
STAT3 , a key regulator of cell survival and proliferation . -
pubmed
Lectins are also one of the promising candidates for useful therapeutic agents because they can recognize the specific
carbohydrate structures such as proteoglycans , glycoproteins , and glycolipids , resulting in the regulation of various cells via glycoconjugates and their physiological and pathological phenomenon through the host - pathogen interactions and cell - cell communications . -
pubmed
PURPOSE :
Amuvatinib is a multi - targeted
tyrosine kinase inhibitor with activity that also disrupts DNA damage repair through suppression of
homologous recombination protein Rad51 . -
pubmed
GE activity was highly specific and hydrolysed the peptide bond predominantly on the
carboxy side of
Glu residues while hydrolysis on the
carboxyl side of
Asp residues was also observed . -
pubmed
Src kinase is a direct target of
apigenin against UVB - induced
skin inflammation . -
pubmed
Apigenin inhibited UVB - induced
cyclooxygenase-2 (
COX-2 ) expression , which is a well - known key mediator of
inflammation and
cancer , and restored the upstream stimulatory factor level in JB6 P+ cells . -
pubmed
Immunoblot and kinase assay data demonstrate that
Src activity was attenuated by
apigenin , and this led to subsequent inhibition of UVB - induced phosphorylation of
epidermal growth factor receptor ,
mitogen - activated protein kinases and
Akt signaling . -
pubmed
In addition , in vitro pull - down assays revealed that
apigenin binds
Src in an
adenosine triphosphate - competitive manner . -
pubmed
Collectively , these findings suggest that
apigenin exerts potent chemopreventive activity against UVB - induced
skin inflammation primarily by targeting
Src . -
pubmed
The synaptic adhesion molecule
SynCAM 1 contributes to
cocaine effects on synapse structure and psychostimulant behavior . -
pubmed
SynCAM 1 additionally contributes to the structural remodeling of NAc synapses in response to the psychostimulant
cocaine . -
pubmed
Specifically , we find that
cocaine administration increases the density of stubby spines on medium spiny neurons in NAc , and that maintaining this increase requires
SynCAM 1 . -
pubmed
This study investigated the role of
Nrf2 in mediating
PHB - induced protection against
colitis and expression of the antioxidant response element ( ARE)-regulated antioxidant genes
heme oxygenase-1 (
HO-1 ) and
NAD ( P ) H quinone oxidoreductase-1 (
NQO-1 ) . -
pubmed
Suberoylanilide hydroxamic acid and
anacardic acid were used to inhibit
histone deacetylase and
histone acetyltransferase , respectively . -
pubmed
Finally , the selective
CXCR2 antagonist
SB225002 was administered intrathecally to reveal the function of spinal
CXCR2 receptors after hind paw incision . -
pubmed
Overall , acetylated
histone H3 at
lysine 9 was increased in spinal cord tissues after incision , and enhanced association of acetylated
histone H3 at
lysine 9 with the promoter regions of
CXCR2 and
keratinocyte - derived chemokine (
CXCL1 ) was observed as well . -
pubmed
Epitaxial monolayer
MoS2 on
mica with novel photoluminescence . -
pubmed
Herein , we have successfully grown predominantly monolayer
MoS2 on an inert and nearly lattice - matching
mica substrate by using a low - pressure chemical vapor deposition method . -
pubmed
Photoluminescence ( PL ) measurements indicate strong single - exciton emission in as - grown
MoS2 and room - temperature
PL helicity ( circular polarization ~0.35 ) on transferred samples , providing straightforward proof of the high quality of the prepared monolayer crystals . -
pubmed
For
cytokine secretion ,
alpha - mangostin ( 1 , 2 and 4 microg / mL ) did not significantly induce either proinflammatory
cytokines ( i.e. -
pubmed
The combination of
alpha - mangostin ( 1 , 2 and 4 microg / mL ) and ConA did not significantly alter the relative difference of
TNF - alpha and
IL-1beta compared with ConA - treated PBMCs ; however , these combinations could significantly decrease the relative difference of
IL-2 compared with ConA - treated PBMCs . -
pubmed
Recent evidence supports the hypothesis that
tinnitus is related to
neuronal hyperactivity in auditory brain regions , and consequently drugs that increase GABAergic neurotransmission in the CNS , such as the
GABA ( B ) receptor agonist
L - baclofen , may be effective as a treatment . -
pubmed
It also lowered plasma
triglyceride ( 4.0 + - 1.6 mmol / L vs 2.6 + - 1.2 mmol / L , p<0.05 ) , but there were no significant changes in
body weight ,
high density lipoprotein cholesterol ,
apolipoprotein A or
apolipoprotein B. -
pubmed
Functional assays and measurements of
cGMP , reactive -
oxygen species ( ROS ) , and
sGC protein expression in USM were determined . -
pubmed
Oxidation of
sGC with
ODQ [
1H -[ 1,2,4 ] oxadiazolo [ 4,3 - a ] quinoxalin-1 - one ] ( 10 muM ) potentiated
BAY 60 - 2770 - induced USM responses in the lean group . -
pubmed
However ,
post hoc analysis showed that MBSR decreased the proportion of participants with at least one flare - up among those with top tertile urinary
cortisol and baseline perceived stress ( 30 vs. 70 % ; p & lt ; 0.001 ) . -
pubmed
GSK1265744 (
GSK744 ) is an
integrase strand - transfer inhibitor that has been formulated as a long - acting ( LA ) injectable suitable for monthly to quarterly clinical administration . -
pubmed
There is an inverse relationship between indirect
bilirubin levels and advanced
liver fibrosis caused by
CHC genotype 1b . -
pubmed
Secondary end points were presence of early
seizures ( 0 to 7 days
post -
TBI ) or late
seizures ( 8 days
post -
TBI to phone interview ) , use of anticonvulsant medication when interviewed , medication - related hospital complications , and a summary of
phenytoin (
PHT ) and
LEV dosing regimens . -
pubmed
Seven - point home blood
glucose monitoring over a 7 - day period in each trimester demonstrated higher fasting and postprandial glycemic excursions in the first trimester of
GCK pregnancies when compared to
HNF-1alpha pregnancies ( fasting 104 [ 90 - 115 ] mg / dL vs 84 [ 77 - 88 ] mg / dL ; P = .01 and postprandial 154 [ 135 - 196 ] mg / dL vs 111 [ 100 - 131 ] mg / dL ; P = .04 ) despite
insulin treatment . -
pubmed
INTRODUCTION : The
adenosine triphosphate - binding cassette transporter A1 (
ABCA1 ) is a major
cholesterol transporter highly expressed in the liver and brain . -
pubmed
RESULTS : After transgenesis , the proliferation of EPCs were increased , and the secretion of
NO ,
LDH ,
iNOS by EPCs were significantly increased compared to the non - transgenesis group . -
pubmed
Donor and recipient demographic data , preoperative
BMI , blood
C - reactive protein (
CRP ) and
adiponectin levels , perivascular adipose tissue ( PAT ) samples and recipient blood
creatinine levels were analyzed . -
pubmed
We show the REMD simulation results of a mixed (
POPC /
DMPC ) lipid bilayer as a real application using GENESIS . -
pubmed
ASH was induced by
ethanol ( 3 times ; 5 g / kg ; 12 h intervals , via gavage ) , followed by a single dose of lipopolysaccharide ( LPS ; 10 mg / kg ; i.p . ) -
pubmed
Reactive
oxygen species formation ,
malondialdehyde levels ,
myeloperoxidase activities and
transforming growth factor beta1 (
TGF - beta1 ) and
collagen 1alpha1 (
COL1A1 ) expressions were observed to decrease . -
pubmed
In the present study ,
danshensu which is a selected component of traditional oriental medicine ( TOM ) compound was found to reduce the radioresistance of
NSCLC by inhibiting the
nuclear factor - kappaB (
NF - kappaB ) pathway . -
pubmed
Taken together , this study shows that
danshensu significantly reduces
MAOB activity and attenuates
NF - kappaB signaling to elicit the radiosensitization of
NSCLC . -
pubmed
Recently ,
hepcidin , an antimicrobial - like peptide hormone , has evolved as the master regulator of
iron homeostasis . -
pubmed
Iron parameters including ( serum
iron ,
ferritin ,
transferrin , and total
iron binding capacity [ TIBC ] ) were also measured . -
pubmed
We found that
AIS cases had higher serum
iron ,
ferritin , and
IL6 levels compared to the control group ( all P & lt ; 0.01 ) . -
pubmed
Serum
hepcidin showed significant positive correlations with serum
iron ,
transferrin saturation ,
ferritin , and
IL6 ( r = 0.375 , P & lt ; 0.05 ; r = 0.453 , P & lt ; 0.05 ; r = 0.687 , P & lt ; 0.01 ; r = 0.515 , P & lt ; 0.01 ; respectively ) . -
pubmed
RESULTS : We verified that chronic administration of
escitalopram decreased the activities of
complexes I and II - III in cerebellum , hippocampus , striatum and posterior cortex whereas prefrontal cortex was not affected . -
pubmed
However , chronic administration of
escitalopram did not affect complex
IV and enzymes of Krebs cycle activities as well as
creatine kinase . -
pubmed
The laboratory prediction model composed of fasting plasma
glucose and
hemoglobin A1c levels showed a significantly higher AROC ( 0.838 , P & lt ; 0.001 ) than the KRS . -
pubmed
The total organic
carbon content measurements confirmed the results obtained by LC /
MS . -
pubmed
Prevalence and significance of
HBeAg and isolated anti -
HBc in several institutions for the
mentally retarded in the autonomous community of madrid . -
pubmed
Of the total , 28 ( 6.8 % ) were
HBsAg positive , and of these , 16 ( 57.1 % ) had a positive test for the
Hepatitis Be antigen (
HBeAg ) . -
pubmed
Proportionately more
Down syndrome chronic carriers of
HBsAg acquired the persistent
Hepatitis Be antigen (
HBeAg ) than
non - Down syndrome patients 15 ( 65.2 % ) vs. 1 ( 20 % ) . -
pubmed
The presence of
HBeAg was correlated with
abnormal liver function and high titres of
HBsAg . -
pubmed
Testing for the
IgM antibody to the
hepatitis B core antigen (
IgM AntiHBc ) facilitated the identification of
acute and chronic hepatitis infection in both RI and NRC individuals . -
pubmed
Targeting
BCR -
ABL+ stem / progenitor cells and
BCR -
ABL - T315I mutant cells by effective inhibition of the
BCR -
ABL -
Tyr177 -
GRB2 complex . -
pubmed
Interestingly , new pre - clinically - validated analogs of
Icaritin (
SNG162 and
SNG1153 ) , which target abnormal
ERalpha36 activity , inhibit cell growth and induce apoptosis of
BCR -
ABL+ leukemic cells , particularly
BCR -
ABL - T315I mutant cells . -
pubmed
Assessment of Efficacy and Irritation of
Ingenol Mebutate Gel 0.015 % Used With or Without
Dimethicone Lotion for Treatment of
Actinic Keratosis on the
Face . -
pubmed
Consistent with the location of the
CSPG4 - binding site on
TcdB , we show that the anti -
TcdB antibody
bezlotoxumab , which binds partially within the first three short repeats , prevents
CSPG4 binding to
TcdB. -
pubmed
Independently from the angle ,
DIP induced decrements in MVC , RFD , and RMS of EMG and MMG signals and lengthened the
EMD components in agonist muscles ( P<0.05 ) . -
pubmed
GC / EI -
MS and
GC / ECNI -
MS mass spectra of the five PMPs were studied with regard to fragmentation pattern and individual responses which were strongly affected by the presence ( or absence ) of
Br in alpha - position(s ) . -
pubmed
A key enzyme of the
shikimate pathway ,
5 - enolpyruvylshikimate-3 - phosphate synthase (
EPSPS ;
EC 2.5.1.19 ) , is the known target of the widely used herbicide
glyphosate . -
pubmed
The aim of this work was to study the pleiotropic effects of ( i )
EPSPS increased transcript abundance due to gene copy number variation ( CNV ) and of ( ii )
glyphosate application on the aromatic
amino acid (
AAA ) and branched chain
amino acid (
BCAA ) synthesis pathways . -
pubmed
Hydroponically grown
glyphosate sensitive (
GS ) and
glyphosate resistant (
GR ) plants were treated with
glyphosate 3 days after treatment . -
pubmed
In absence of
glyphosate treatment , high
EPSPS gene copy number had only a subtle effect on transcriptional regulation of
AAA and
BCAA pathway genes . -
pubmed
In contrast ,
glyphosate treatment provoked a general accumulation of the transcripts corresponding to genes of the
AAA pathway leading to synthesis of
chorismate in both
GS and
GR . -
pubmed
After
chorismate ,
anthranilate synthase transcript abundance was higher while
chorismate mutase transcription showed a small decrease in
GR and remained stable in
GS , suggesting a regulatory branch point in the pathway that favors synthesis toward
tryptophan over
phenylalanine and
tyrosine after
glyphosate treatment . -
pubmed
Importantly , this upregulation was
glyphosate dose dependent and was observed similarly in both
GS and
GR populations . -
pubmed
palmeri
GR populations has no major pleiotropic effect on the expression of
AAA biosynthetic genes , even in response to
glyphosate treatment . -
pubmed
As a result , a distinct colorimetric signal is produced by the
ABTS oxidation promoted by the
peroxidase mimicking activity of the released G - quadruplex DNAzymes . -
pubmed
Communication : Mode - dependent excited - state lifetime of
phenol under the
S1 / S2 conical intersection . -
pubmed
The reaction barrier formed by the conical intersection between the optically
bright S1 ( pipi * ) state and the dissociative S2 ( pisigma * ) state does not inhibit
O - H bond
rupture even though the excitation energy is below the barrier height . -
pubmed
To examine the
O - H bond -
rupture dynamics in association with the initial excited vibrational modes , we used a picosecond laser to investigate the vibrational - mode - dependent excited - state lifetime of
phenol under the
S1 / S2 conical intersection . -
pubmed
Unexpectedly short lifetimes were observed in the
S1 state for a '' symmetric vibrational modes ( including v4 , v16a ,
tauOH , and v5 ) . -
pubmed
In this work , a wide range of
1,2,3 - triazole - chromenone carboxamides were designed , synthesized , and evaluated for their
cholinesterase inhibitory activity . -
pubmed
Among them ,
N -( 1 - benzylpiperidin-4 - yl )- 7 -(( 1 -( 3,4 - dimethylbenzyl )- 1H-1,2,3 - triazol-4 - yl ) methoxy )- 2 - oxo-2H - chromene-3 - carboxamide ( 11b ) showed the best
acetylcholinesterase inhibitory activity ( IC50 = 1.80 microM ) , however , it was inactive toward
butyrylcholinesterase . -
pubmed
Potassium level before dialysis ( OR = 0.60 ) ,
parathyroid hormone ( OR = 0.99 ) , and higher Kt / V ( OR = 0.28 ) were found to be an advantage for survival . -
pubmed
The antibacterial effect of
fluoxetine was determined by a broth microdilution method ( the M07 -
A9 method of the Clinical and Laboratory Standard Institute ) and flow cytometry techniques in which the probable mechanism of action of the compound was also assessed . -
pubmed
Body mass index (
BMI ) ,
glycated hemoglobin ( HbA1c ) , fasting blood
glucose ( FBG ) , and lipid level , as well as Mini - Mental State Examination and Berg Balance Scale scores , were measured at baseline and postintervention . -
pubmed
In our study , we detected that oxidant parameter values and
cytokine levels increased in the negative control group , but total antioxidant status reduced , whereas ,
cytokine and oxidant parameter levels were significantly reduced due to low and high doses of
caftaric acid administration . -
pubmed
Vitamin B12 modulates
Parkinson ' s disease LRRK2 kinase activity through allosteric regulation and confers neuroprotection . -
pubmed
However , nearly all inhibitors are
ATP competitive - some with unwanted side effects and unclear clinical outcome - alternative types of
LRRK2 inhibitors are lacking . -
pubmed
Herein we identify
5 '- deoxyadenosylcobalamin (
AdoCbl ) , a physiological form of the essential micronutrient
vitamin B12 as a mixed - type allosteric inhibitor of
LRRK2 kinase activity . -
pubmed
Multiple assays show that
AdoCbl directly binds
LRRK2 , leading to the alterations of protein conformation and
ATP binding in
LRRK2 . -
pubmed
STD - NMR analysis of a
LRRK2 homologous kinase reveals the contact sites in
AdoCbl that interface with the kinase domain . -
pubmed
Furthermore , we provide evidence that
AdoCbl modulates
LRRK2 activity through disrupting
LRRK2 dimerization . -
pubmed
Our study uncovers
vitamin B12 as a novel class of
LRRK2 kinase modulator with a distinct mechanism , which can be harnessed to develop new
LRRK2 - based PD therapeutics in the future . -
pubmed
One
hydrochlorofluorocarbon and two
hydrofluorocarbons (
HCFC-22 ,
HFC-125 , and
HFC-152a ) were measured in air samples at the Cape Point observatory (
CPT ) , South Africa , during 2017 . -
pubmed
The components analysed were major
STP components such as water ,
nicotine ,
sugars , humectants ,
sodium ions ,
chloride ions and ash . -
pubmed
The
HA protein of viruses from each cluster showed a different number of basic
amino acids ( n = 5 - 7 ) in the cleavage site , and six different patterns at the predicted N - glycosylation sites . -
pubmed
The Dephosphorylation of
p70S6 (
Thr389 ) Kinase as a Marker of
L - Glutamate - Induced
Excitotoxicity Related to
Diabetes Disturbances - an Unconventional In Vitro Model . -
pubmed
The three reagents were used in different concentrations to create the model of excitotoxicity related to
diabetes disturbances :
L - glutamate ( 2.5 mM ; 10 mM ) ,
glucose ( 150 mM ; 200 mM ) , and
insulin ( 0.093 mM ; 0.371 mM ) . -
pubmed
The results we obtained demonstrated the increased phosphorylation of
p70S6 K in
Thr389 residue in almost all combinations of reagents , except for those including the highest concentration of
L - glutamate , in which dephosphorylation was confirmed . -
pubmed
This confirms the inhibition of
mTOR kinase and suggests that
p70S6 K (
Thr389 ) plays a functional role in the regulation of the signaling pathway in excitotoxicity related to
diabetic disturbances . -
pubmed
Control of the redox potential of
lithium terephthalate Li2TP anode material is demonstrated by functionalizing its
terephthalate backbone with an electron - donating
amino group ; this lowers - as intended - the redox potential of
Li2TP by 0.14 V. The two
Li - organic electrode materials ,
Li2TP and
Li2TP - NH2 , are fabricated as crystalline thin films from gaseous precursors using the atomic / molecular layer deposition ( ALD /
MLD ) technique . -
pubmed
From galvanostatic cycling experiments and
post - mortem XRD and SEM analysis , the issue with
Li2TP - NH2 is revealed to be in the morphology changes occurring during the discharge / charge cycling . -
pubmed
Only 79 % of
Pt mine workers , used their respiratory protective equipment (
RPE ) , sixty - five percent were exposed to work shifts exceeding the recommended eight hours and 8.8 % had been employed for more than ten years . -
pubmed
The
Pt miners had increased health risks due to accumulated low levels of dust exposure and lack of usage of
RPE . -
pubmed
Antidepressant - Like Effects and Cognitive Enhancement of Coadministration of Chaihu Shugan San and
Fluoxetine : Dependent on the
BDNF -
ERK -
CREB Signaling Pathway in the Hippocampus and Frontal Cortex . -
pubmed
Then , the
depression - like behavior and cognitive function were evaluated by the
sucrose preference test ( SPT ) , forced swimming test (
FST ) , open field test ( OFT ) , and Y - maze test . -
pubmed
What is more , the antidepressant mechanism of CSS and coadministration of CSS and
FLU were studied through
BDNF mRNA ,
ERK mRNA , CREB mRNA ,
BDNF , p -
ERK /
ERK , and p - CREB /
CREB levels in the hippocampus and frontal cortex by Western blot and RT - PCR . -
pubmed
Finally , we found that both CSS and coadministration of CSS and
FLU play an antidepressant role , which may be due to the regulation of the
BDNF /
ERK /
CREB signaling pathway in the hippocampus and frontal cortex . -
pubmed
We used this
tryptophan to determine the binding affinity of the unlabeled
IkappaBalpha peptide to
14 - 3 - 3 via
tryptophan fluorescence decay measurements . -
pubmed
MATERIALS AND METHODS : We studied the anti -
HCC effect of
FH535 and a novel derivative
Y3 , on proliferation , mitochondrial function and cellular metabolism focusing on the three key substrates ,
glutamine ,
glucose , and
fatty acids . -
pubmed
These
sulfonamides ,
FH535 and
Y3 , targeted
glutamine and
fatty acid metabolism and caused
HCC cell reprograming towards the preferential use of
glucose and the glycolytic pathway . -
pubmed
CONCLUSIONS :
FH535 , and
Y3 , demonstrated potent anti -
HCC activity by targeting OXPHOS , increasing dangerous levels of ROS and reducing
ATP production . -
pubmed
These
sulfonamides target
glutamine and
FA metabolic pathways significantly increasing the cellular dependency on glycolysis . -
pubmed
OBJECTIVE : This study aimed to determine how the
GABAa and GABAb receptors of the central nucleus of the amygdala ( CeA ) regulate the compulsive
cocaine - seeking behavior . -
pubmed
The compulsive
cocaine - seeking behavior was measured as the
cocaine - seeking behavior in the
face of footshock punishment . -
pubmed
The role of the
GABA receptors of CeA in the regulation of such behavior was determined by measuring the dose - dependent effects of the
GABAa agonist
muscimol or the GABAb agonist
baclofen bilaterally microinjected into the CeA on the punished
cocaine - seeking behavior . -
pubmed
CONCLUSION : These data indicate that the CeA
GABAa receptors play a key role in the regulation of the compulsive
cocaine - seeking behavior and suggest that an increase in the function of the
GABAa receptors possibly induced by
cocaine or genetic factors may be an important mechanism involved in the development of or vulnerability to the compulsive
cocaine use and
addiction . -
pubmed
Characterization of four
BCHE mutations associated with prolonged effect of
suxamethonium . -
pubmed
Here , we report the characterization of four
BCHE mutations associated with prolonged effect of
suxamethonium (
amino acid numbering based on the matured enzyme ): p.20delValPheGlyGlyThrValThr , p. Leu88His , p. Ile140del and p. Arg386Cys . -
pubmed
Cartilage was treated enzymatically to extract
collagen , analyzed for
14C content , and year at formation was determined from historical atmospheric
14C concentrations . -
pubmed
The target of
rapamycin (
TOR ) is an evolutionarily - conserved
serine /
threonine kinase that senses and integrates signals from the environment to coordinate developmental and metabolic processes . -
pubmed
A decrease in serum
phosphate (
PO43- ) levels , which in most cases is asymptomatic , has been reported with
IV iron preparations . -
pubmed
The
face shield frames were manufactured using the 3DVerkstan design and were coupled with an
acetate sheet to assemble a complete
face shield . -
pubmed
We highlight that the
MeCP2B isoform is required for correct higher - order PCH organization , and underline involvement of the
methyl - binding and transcriptional repression domains . -
pubmed
The administration of
monosodium glutamate (
MSC ) to chick embryos at 24 hours did neither produce gross
developmental defects nor influence growth by 72 hours total incubation . -
pubmed
Zinc ions were shown to reversibly inhibit the activity of
Pseudomonas protease . -
pubmed
To characterize the site or sites of initiation of this
tRNA transcription ,
rifampicin - resistant complexes of
RNA polymerase , DNA , and either
ATP (
UTP and
CTP ) or
GTP (
UTP and
CTP ) were formed , and tRNA was transcribed from these stabilized sites . -
pubmed
It is concluded that the initiation of
tRNA transcription can occur , without accessory factors , with the insertion of
ATP at a single or a few closely spaced sites , and that the tRNAs encoded by the bacteriophage T4 are present in a single operon . -
pubmed
thermophilus
PheRS , a heterotetrameric ( alpha 2
beta 2 ) ,
class II aminoacyl - tRNA synthetase . -
pubmed
Intraperitoneal injection of
caerulein did not change the basal c - fos and
zif / 268 expression . -
pubmed
Pretreatment with
caerulein suppressed the
PTZ - induced c - fos and
zif / 268 expression . -
pubmed
Nonphorbol tumor promoters
okadaic acid and
calyculin - A induce membrane translocation of
protein kinase C. -
pubmed
The cell - permeable inhibitors of
type 1 and 2A protein phosphatases ,
okadaic acid and
calyculin - A , induced a redistribution of
protein kinase C (
PKC ) activity and immunoreactivity ( 40 to 60 % ) from cytosol to membrane in some cell types . -
pubmed
Calyculin - A was 100 - fold more potent than
okadaic acid and required only 5 to 10 nM concentrations to induce this
PKC translocation . -
pubmed
Taken together these results suggest that although the
okadaic acid class of
tumor promoters and
phorbol ester - related agents bind to two different cellular receptors having counteracting enzymic activities , they share a common mechanism of action , namely the induction of cytosol to membrane translocation of
PKC . -
pubmed
A Kd of 1.72 + /- 0.12 nM for
hSBP showed that the receptor binding site was effective for both
androgen - carrier molecules . -
pubmed
The receptor complex was also abolished in the presence of a 100 - fold excess of either unlabelled
rABP or unlabelled
hSBP , or in the presence of 20 mM
EDTA . -
pubmed
The intermediate V is then reduced to
1 - chloro-3,4 - dihydroxybenzene (
II ) , and the latter is methylated to form
1 - chloro-3,4 - dimethoxybenzene ( VI ) . -
pubmed
VI in turn is oxidized by LiP to generate
chloride and
2 - methoxy-1,4 - benzoquinone (
VII ) , which is reduced to
2 - methoxy-1,4 - dihydroxybenzene (
IV ) . -
pubmed
IV is oxidized by either
LiP or
MnP to generate
4 - hydroxy-1,2 - benzoquinone , which is reduced to
1,2,4 - trihydroxybenzene ( III ) . -
pubmed
This intermediate is also generated during the
LiP- or
MnP - catalyzed oxidation of the intermediate
chlorocatechol (
II ) . -
pubmed
The former is recycled by reduction and methylation reactions to generate an intermediate which is also a substrate for
peroxidase - catalyzed oxidation , leading to the removal of a second
chlorine atom . -
pubmed
The effect of a single dose of
carbon tetrachloride (
CT ) ,
1,2 - dichloroethane (
DCE ) and the mixture on
liver toxicity was investigated . -
pubmed
Several of the bands identified by
SDS - PAGE were composed of
glycoproteins or
carbohydrates as determined by their affinity for
concanavalin A. -
pubmed
Four of the antigenic determinants recognized by patient sera reacted with the
concanavalin A - peroxidase stain , indicating that they are composed of
glycoproteins or
carbohydrate . -
pubmed
These
CO doses produced
HbCO concentrations of 12 , 24 , 35 , 45 , and 60 % , respectively , at 30 min postinjection . -
pubmed
The susceptibility of a variety of
oligosaccharides to
endo - beta - N - acetylglucosaminidase D was investigated . -
pubmed
The
oligosaccharides having the structures of
Man alpha 1 - --- 6 ( GlcNAc beta 1 - --- 4Man alpha 1 - --- 3 ) Man beta 1 - --- 4GlcNAc beta 1 - --- 4 (+/- Fuc alpha 1 - --- 6 ) GlcNAcOT , derived from complex type triantennary
sugar chains , released + /-
Fuc alpha 1 - --- 6GlcNAcOT upon incubation with the
enzyme at almost the same rate as
Man alpha 1 - --- 6 ( Man alpha 1 - --- 3 ) Man beta 1 - --- 4GlcNAc beta 1 - --- 4GlcNAcOT . -
pubmed
These results indicated that
endo - beta - N - acetylglucosaminidase D does not require the presence of a free
hydroxyl group at the C-4 position of the
alpha - mannosyl residue of the
trisaccharide glycon :
Man alpha 1 - --- 3Man beta 1 - --- 4GlcNAc beta 1 - --- . -
pubmed
Studies with 125I - labeled
NIDS protein indicated that the main precipitate on 2 - dimensional immunoelectrophoresis was associated with
SDS -
polyacrylamide gel components of molecular weight 15,000 and 30,000 . -
pubmed
Oral ( 100 g ) or intravenous ( 0.5 g X kg-1 )
glucose was used as a stimulus for
insulin secretion in both studies . -
pubmed
Neither acute nor chronic
cimetidine administration modified
insulin secretion and
glucose tolerance . -
pubmed
These data are consistent with the idea that
H2 - receptors are not involved in the insulinogenic effect of
glucose . -
pubmed
The optimal dose for induction , as measured by the increase of the number of cells containing
sheep erythrocyte receptors ( E - rosette assay ) , is between 8 and 16 nM
12 - O - tetradecanoylphorbol 13 - acetate (
TPA ) , although there were significant increases of E - rosette - positive ( E(+ ) ) cells at concentrations as low as 1.6 nM
TPA . -
pubmed
Finally , we have shown that
TPA also affected another differentiation marker , the loss of the enzyme
terminal deoxyribonucleotidyl transferase . -
pubmed
The sequence of the 89 - residue form of
HTa is :
H2N - Val - Gly - Ile - Ser - Glu - Leu - Ser - Lys - Glu - Val - Ala - Lys - Lys - Ala - Asn - Thr - Thr - Gln - Lys - Val - Ala - Arg - Thr - Val - Ile - Lys - Ser - Phe - Leu - Asp - Glu - Ile - Val - Ser - Glu - Ala - Asn - Gly - Gl y - Gln - Lys - Ile - Asn - Leu - Ala - Gly - Phe - Gly - Ile - Phe - Glu - Arg - Arg - Thr - Gln - Gly - Pro - Arg - L ys - Ala - Arg - Asn - Pro - Gln - Thr - Lys - Lys - Val - Ile - Glu - Val - Pro - Ser - Lys - Lys - Lys - Phe - Val - Phe - Arg - Ala - Ser - Ser - Lys - Ile - Lys - Tyr - Gln - Gln - COOH The molecular weight calculated from the sequence is 9,934 . -
pubmed
Another form of
HTa probably differs only by the presence of an additional residue (
methionine ) at the NH2 terminus ( the calculated molecular weight of this form is 10,065 ) . -
pubmed
The relationship to
renin -
angiotensin system and
electrolyte metabolism ( author 's transl ) ] . -
pubmed
The author is of the opinion that the
renin -
angiotensin -
aldosterone system might be related to these changes during
respiratory acidosis . -
pubmed
These reports prompted me to question whether the
renin -
angiotensin -
aldosterone system was related to the pathophysiological evidence concerning the
respiratory acidosis . -
pubmed
It is generally accepted that plasma
aldosterone concentration is controlled by ( 1 ) the
renin - angiotensin system , ( 2 )
ACTH and ( 3 ) serum
potassium . -
pubmed
Therefore , the purpose of this experiment was to investigate the roles of the
renin - angiotensin system and
electrolyte metabolism on plasma
aldosterone concentration during acute
respiratory acidosis . -
pubmed
The increase of plasma
aldosterone concentration was delayed and blurred , in contrast with a significant increase of
plasma renin activity , during the acute
respiratory acidosis , due to delayed time course of
angiotensin II stimulation and also the expected changes in the concentration of
angiotensin II receptors on the
zona glomerulosa in the adrenal cortex . -
pubmed
There was a possibility that the increase in the reabsorption of
sodium and water in the proximal tubules , and the increase of
ADH secretion during the acute
respiratory acidosis , could be related to a decrease in excreted
sodium and
potassium as well as urine volume . -
pubmed
These results suggest that the changes of plasma
aldosterone concentration may be affected partially by
renin -
angiotensin system but not by serum
potassium , and plasma
aldosterone should be a minimal determinant on the electrolyte metabolism during
respiratory acidosis with 10 %
CO2 inhalation . -
pubmed
Specific DV
antigen was revealed by the fluorescent antibody (
FA ) technique in cells located in the infected tissues . -
pubmed
Factor 2 was negatively correlated with UNaV : its correlations with plasma protein concentration , haematocrit , blood pressure ,
glomerular filtration rate and renal plasma flow implied that it represented
post - glomerular plasma protein concentration and its influence on
sodium reabsorption . -
pubmed
The time course of
haemoglobin autoxidation was studied under various conditions at 37 degrees C , and the changes in
oxyhaemoglobin , intermediate
haemoglobins and methaemoglobin during the reaction were analysed by isoelectric focusing on
Ampholine /
polyacrylamide - gel plates . -
pubmed
Under various conditions ( 10 mM -
phosphate buffer , 10 mM -
phosphate buffer with 0.1 M -
phosphate buffer , 10 mM -
phosphate buffer with 0.1 M -
NaCl , and 10 mM -
phosphate buffer with 0.5 mM -
inositol hexaphosphate ; pH range 6.6 - 7.8 each case ) , the intermediate
haemoglobins were found to be present as ( alpha 2 + beta 3+)2 and ( alpha 3 + beta 2+)2 valency hybrids from their characteristic positions on electrophoresis . -
pubmed
The modes of the quantitative changes in
oxyhaemoglobin , intermediate
haemoglobins , and methaemoglobin were very similar in all the media used except for the
inositol hexaphosphate addition . -
pubmed
In the presence of
inositol hexaphosphate , the autoxidation rates were considerably accelerated , and the modes of the changes in the
haemoglobin derivatives were also considerably altered ; the effects of this organic
phosphate were maximal at acidic pH and minimal at alkaline pH. -
pubmed
Preliminary analysis has shown possible evidence of benefit for both these clinical indications , and several other
glutamate antagonists are now being evaluated for these indications in Phase
II trials . -
pubmed
Inhibitory effect of
CP-96,345 , a non - peptide
neurokinin-1 - receptor antagonist , on neurogenic responses of guinea - pig isolated airway smooth muscle . -
pubmed
The actions of
CP-96,345 , a non - peptide
neurokinin-1 receptor antagonist , on the responses evoked by electrical - field stimulation or by
acetylcholine and
substance P in guinea - pig tracheal and bronchial muscle strips were examined . -
pubmed
Submaximal contractions of tracheal and bronchial muscles evoked by exogenous
substance P ( 0.1 - 3 microM ) were partly inhibited by
CP-96,345 ( 1 - 10 microM ) , but
acetylcholine - induced contractions were hardly inhibited . -
pubmed
Stimulation by
galactose of protein synthesis in the presence of the
heme precursor
5 - aminolevulinic acid peaked 72 h after addition to the inducer to yield 108 nmol membrane - bound
P-450 2B5 per liter of culture medium . -
pubmed
The recombinant
enzyme was purified to near homogeneity by a two - column procedure involving chromatography on DE-52 cellulose and
hydroxylapatite . -
pubmed
The
hemoprotein was isolated mainly in the low - spin
iron configuration and exhibited a reduced
CO - difference spectrum with a Soret band at 451 nm . -
pubmed
Second - derivative spectral analysis in the middle - UV region revealed that type I binding of
4 - nitroanisole to
ferric P-450 2B5 abolished absorption bands ascribable to
tyrosine residues within the polypeptide chain . -
pubmed
Pseudo - first - order rates of
NADPH - driven reduction of the pigment were lower when reconstituted with
NADPH - cytochrome P-450 reductase than with the mitochondrial
adrenodoxin / NADPH - adrenodoxin reductase redox couple . -
pubmed
The
enzyme was catalytically active toward
4 - nitroanisole and
androstenedione ; metabolic rates were enhanced to different extents by the presence of
cytochrome b5 . -
pubmed
The recombinant
hemoprotein did not catalyze bioactivation of
4 -( N - methyl - N - nitrosamino )- 1 -( 3 - pyridyl )- 1 - butanone , a potent pulmonary carcinogen . -
pubmed
A KEKE motif , consisting of a very hydrophilic domain rich in ' alternating '
lysine ( positive ) and
glutamate ( negative ) residues , is present in the C - terminus of
p40 . -
pubmed
Calcium - dependent inactivation of
RNA polymerase III transcription . -
pubmed
We have found that these extracts no longer support
RNA polymerase III transcription in response to a brief incubation in the presence of
Ca2 + . -
pubmed
Fractionation of extracts was used to show that
transcription factors ( TF ) IIIC and , to a lesser extent ,
TFIIIB , but not
RNA polymerase III , were targets of the
Ca ( 2 +)-dependent inactivation process . -
pubmed
An additional component ( not present in fractionated
TFIIIC or
TFIIIB ) was required for the
Ca ( 2 +)-dependent destruction of
transcription factor activity . -
pubmed
The
Ca ( 2 +)-dependent inactivation process was blocked by
protease inhibitors that inhibit known
Ca ( 2 +)-dependent
proteases called calpains . -
pubmed
The common use of
Ca2 + as a second messenger and the widespread distribution of calpains suggest that the proteolytic degradation of
transcription factors may be a general mechanism for the regulation of gene expression . -
pubmed
whether the variability in manifestations is due to variable expressions of
steroid 5 alpha - reductase 1 or to effects of
testosterone itself . -
pubmed
To evaluate further the possible differential expression of
bFGF in
melanocytic lesions , we examined 110
formalin - fixed , paraffin - embedded metastatic and primary
invasive melanomas ,
melanomas in situ ,
nevi with
architectural disorder and
cytological atypia , and ordinary benign
melanocyte nevi by nucleic acid in situ hybridization . -
pubmed
In addition ,
defensin increased the rate of monocyte aggregation induced by
arachidonic acid or
phorbol myristate acetate . -
pubmed
Oxidation of MA was completely inhibited by 0.1 - 1 mM
semicarbazide , without being affected by the
monoamine oxidase (
MAO ) inhibitor ,
pargyline ( 1 mM ) , indicating that MA is metabolized by
semicarbazide - sensitive amine oxidase (
SSAO ) and not by
MAO . -
pubmed
These results are similar to previous findings that
SSAO in vascular smooth muscle is able to metabolize
aliphatic amines such as MA , and furthermore suggest that
SSAO may play a role in adipose tissue function and/or maturation . -
pubmed
The localization of
riboflavin sulfates in deficient roots is similar , but not identical , to that of high
iron reductase activity . -
pubmed
We have previously shown that the high - affinity binding of
substance P ( SP ) to its receptor is dependent on an interaction with a
PTX - insensitive
G protein . -
pubmed
Photoincorporation of this SP derivative was susceptible to
guanine nucleotide inhibition , indicating that the receptor was coupled to its
G protein during labeling . -
pubmed
Activation of nuclear
IRF-1 GAS - binding activity by
sIg was inhibited by the
tyrosine kinase inhibitor ,
herbimycin A , and binding activity was eliminated by
tyrosine phosphatase treatment . -
pubmed
This heretofore described
cytokine signaling pathway may play a role in bringing about
Ag - specific proliferative and differentiative responses . -
pubmed
However , a 28 % increase in plasma
high density lipoprotein (
HDL)-
cholesterol ( P & lt ; .01 ) was noted during the initial 12 - week training period , which regressed during the washout period , and was not replicated during the second 12 - week exercise period . -
pubmed
A
purine /
pyrimidine mirror repeat element ( M - PMR3 ) in the
MUC1 promoter has been shown to form H - DNA under in vitro conditions . -
pubmed
TS1 , the CBD , and an
IAP agonist peptide ( 4N1 K ) from the CBD of
TS1 activate the
platelet integrin alphaIIbbeta3 , resulting in platelet spreading on immobilized
fibrinogen , stimulation of
platelet aggregation , and enhanced
tyrosine phosphorylation of
focal adhesion kinase . -
pubmed
Corticosterone levels were positively correlated with
fentanyl SA . -
pubmed
Predictability of the stressor also had a greater effect on maintenance of
fentanyl SA than it did on
relapse to
fentanyl SA . -
pubmed
Annexin V binding to
phosphatidylserine was evaluated by flow cytometry to examine apoptosis in different lymphocyte subsets of peripheral blood mononuclear cells after a 24 h in vitro culture period . -
pubmed
The antibody binding capacity ( ABC ) of
lymphocyte membrane antigens was determined with
CD3 -
fluorescein isothiocyanate (
FITC ) ,
CD45RA - FITC ,
CD4 - phycoerythrin (
PE ) ,
CD8 -
PE ,
CD56 -
PE , and
CD20 -
PE in viable and apoptotic cells . -
pubmed
In the apoptotic cells a decrease of
ABC was found for all
antigens , except for
CD20 . -
pubmed
The results showed that
silicone gel # 3 ( McGhan lot # S0400488 ) produced the highest mean
anti - OVA antibody titer followed by
silicone gel # 1 ( DC lot # HH019581 ) and
silicone gel # 2 ( McGhan lot # DP9339 ) . -
pubmed
However , because
EGTA does not inhibit splicing , the involvement of
CaMK II itself in splicing is unlikely ; rather , a protein similar to
CaMK II may be involved in spliceosome assembly and splicing . -
pubmed
Cytokine and
prostaglandin production by amnion cells in response to the addition of different bacteria . -
pubmed
Samples for quantification of
interleukin-1 beta ,
interleukin-6 ,
interleukin-8 ,
tumor necrosis factor - alpha , and
prostaglandin E2 were collected at 6 , 12 , 24 , and 48 hours . -
pubmed
coli enhanced the secretion of
interleukin-8 and also induced an transient increase of
prostaglandin E2 in a dose - dependent manner . -
pubmed
aureus did not cause an increase in either
interleukin-6 ,
interleukin-8 , or
prostaglandin E2 . -
pubmed
However , the cytotoxic activity of
SBA was only slightly modified by
iron ,
cysteine analog or
catalase , in contrast to
ascorbate , which was highly sensitive to all these agents . -
pubmed
Therefore , the ability of one such anionic
phospholipid ,
phosphatidic acid (
PA ) , to enhance the activity of this enzyme has been investigated in detail . -
pubmed
The enhancement of hydrolysis in the presence of
PA could also be achieved by prior treatment of pure PC vesicles with
PLD , an effect that was dependent on the concentration of
PLD and the duration of exposure to this
enzyme . -
pubmed
BQ-123 is extensively used as an antagonist at
endothelin ( ET ) receptors , having selectivity at the
ET ( A ) receptor subtype . -
pubmed
In contrast , the
endothelin receptor antagonists ,
BQ-788 ,
PD155080 and
PD145065 ( 1 - 10 microM ) did not affect the L - type
Ca2 + current . -
pubmed
The mechanism of action of
BQ-123 , therefore , is not confined to
ET receptor antagonism . -
pubmed
Three major players have been presented over the past years : ( 1 ) an inward - directed pump specific for
phosphatidylserine and
phosphatidylethanolamine , known as
aminophospholipid translocase ; ( 2 ) an outward - directed pump referred to as '
floppase ' with little selectivity for the polar headgroup of the
phospholipid , but whose actual participation in transport of endogenous lipids has not been well established ; and ( 3 ) a
lipid scramblase , which facilitates bi - directional migration across the bilayer of all phospholipid classes , independent of the polar headgroup . -
pubmed
Whereas a concerted action of
aminophospholipid translocase and
floppase could , in principle , account for the maintenance of lipid asymmetry in quiescent cells , activation of the
scramblase and concomitant inhibition of the
aminophospholipid translocase causes a collapse of lipid asymmetry , manifested by exposure of
phosphatidylserine on the cell surface . -
pubmed
Finally ,
phosphatidylserine exposure during apoptosis will be briefly discussed in relation to inhibition of
translocase and simultaneous activation of
scramblase . -
pubmed
Phox2a is not only necessary but also sufficient to induce
Phox2b+
dopamine - beta - hydroxylase+ and
tyrosine hydroxylase+ NA neurons in ectopic locations . -
pubmed
Inhibition of
NHE was achieved by hypotonic stress and by using the unspecific
NHE inhibitor ,
amiloride , or the specific
NHE 1 inhibitor ,
cariporide (
HOE 642 ) . -
pubmed
Hypotonic stress inhibited basal bile flow by 46 % and prevented
secretin stimulation of bile flow by reducing biliary
bicarbonate output by 50 % . -
pubmed
Secretin increased bile flow from 3.7 + /- 0.8 microL / min / g to 4.78 microL / min / g ( P & lt;.01 ) ; subsequent exposure to hypotonic stress decreased
secretin - stimulated bile flow by 35 % and biliary
bicarbonate secretion by approximately 50 % . -
pubmed
Inhibition of
NHE by
amiloride or
cariporide resulted in a similar reduction of
secretin - stimulated bile flow and
bicarbonate secretion . -
pubmed
In contrast , hypotonic exposure inhibited
Cl (- ) ,
HCO ( 3 )(- ) exchange activity in isolated BECs only during basal conditions but incompletely after
secretin stimulation . -
pubmed
Properties of an
insulin receptor with an
IGF-1 receptor loop exchange in the
cysteine - rich region . -
pubmed
Use of
rifabutin with
protease inhibitors for
human immunodeficiency virus - infected patients with
tuberculosis . -
pubmed
RESULTS :
Niacin use significantly increased
HDL - C by 29 % and 29 % and decreased
triglycerides by 23 % and 28 % and
low - density lipoprotein cholesterol (
LDL - C ) by 8 % and 9 % , respectively , in participants with and without
diabetes ( P<.001 for
niacin vs placebo for all ) . -
pubmed
Corresponding changes in participants receiving placebo were increases of 0 % and 2 % in
HDL - C and increases of 7 % and 0 % in
triglycerides , and increases of 1 % and 1 % in
LDL - C.
Glucose levels were modestly increased by
niacin ( 8.7 and
6.3 mg / dL [ 0.4 and 0.3 mmol / L ] ; P = .04 and P<.001 ) in participants with and without
diabetes , respectively . -
pubmed
Levels of
HbA(1c ) were unchanged from baseline to follow - up in participants with
diabetes treated with
niacin . -
pubmed
Alleles of
glpR associated with the same
GlpR(- ) phenotype produce substitutions in different conserved portions of the
glycerol 3 - phosphate repressor which are not part of the helix - turn - helix motif . -
pubmed
Reaction of
nitric oxide with synthetic hemoprotein ,
human serum albumin incorporating
tetraphenylporphinatoiron ( II ) derivatives . -
pubmed
The reaction of
nitric oxide (
NO ) with a synthetic hemoprotein , the
recombinant human serum albumin (
rHSA ) incorporating eight
tetraphenylporphinatoiron ( II ) derivatives bearing a covalently linked axial base (
FeP ) [ rHSA -
FeP ] , has been investigated . -
pubmed
ESR spectroscopy revealed that the incorporated
FePs in the
albumin formed six - coordinate
nitrosyl complexes ; the proximal
imidazole moiety does not dissociate from the central
iron when
NO binds to the trans side . -
pubmed
Since
NO - association is diffusion controlled , incorporation of the synthetic
heme into the
albumin matrix appears to restrict the
NO access to the central
iron ( II ) . -
pubmed
This has led to a growing consensus that plasma total and
low - density lipoprotein cholesterol (
LDL - C ) should be reduced to target levels that have been shown in population studies to be associated with low rates of
coronary heart disease (
CHD ) . -
pubmed
The aim of this study is to clarify whether the activation of a
Na (+)/ H (+ ) exchanger (
NHE ) is tightly concerned with neuronal and glial cell
injury induced by
ischemia using a selective
NHE inhibitor ,
SM-20220 (
N -( aminoiminomethyl )- 1 - methyl-1H - indole-2 - carboxamide methanesulfonate ) . -
pubmed
SM-20220 significantly reduced
LDH release in both cells in a concentration - dependent manner , and this effect was statistically significant at concentrations of more than 10(-8 ) mol / l for neurons and 10(-7 ) mol / l for glia . -
pubmed
CONCLUSIONS : These data indicate that
delta9 - THC selectively impairs performance of a working memory task through a
CB , receptor mechanism of action and that these memory disruptions are more sensitive than other pharmacological effects of
delta9 - THC . -
pubmed
The
As2O3 - treated cells were examined for
glutathione (
GSH ) level and activity of
catalase (
CAT ) ,
superoxide dismutase (
SOD ) ,
glutathione S - transferase (
GST ) and
glutathione peroxidase (
GPX ) . -
pubmed
However , activity of
GPX ,
GST and
SOD did not significantly correlate with their resistance to
As2O3 - induced apoptosis . -
pubmed
CONCLUSION : Intracellular
GSH level and/or
catalase activity are important factors to determine sensitivity of malignant hematopoietic cells to
As2O3 - induced apoptosis . -
pubmed
In the concentration range 0.5 to 2 mM , ( + /-)-
isoflurane inhibited ( 45)
Ca ( 2 + ) fluxes and functionally modulated the effects of the
Ca ( 2 + ) channel antagonist
nifedipine ( 1 - 10 microM ) . -
pubmed
Isoflurane - induced inhibition of
( 45 ) Ca ( 2 + ) fluxes was not significantly affected by pretreatment with either
pertussis toxin ( 5 microg / ml ) or
phorbol 12 - myristate 13 - acetate ( 50 nM ) . -
pubmed
In conclusion , results indicate that
isoflurane , independent of intracellular
Ca ( 2 + ) levels , nonstereospecifically inhibits the function of
voltage - dependent Ca ( 2 + ) channels and this effect is mediated through multiple binding sites . -
pubmed
This selective induction of apoptosis , which we detect by FACS analysis of intracellular HIV /
p24 and concomitant surface and apoptosis marker expression , is abrogated by the
glutathione precursor ,
N - acetyl - l - cysteine . -
pubmed
Enhancement of
cytarabine sensitivity in
squamous cell carcinoma cell line transfected with
deoxycytidine kinase . -
pubmed
The reason for this is thought to be that in cell lines of solid
tumors the expression of
cytidine deaminase , an enzyme that degrades
cytarabine , is high , whereas the expression of
deoxycytidine kinase (
dCK ) , which phosphorylates
cytarabine ( a prodrug ) , is weak . -
pubmed
CONCLUSION : The
dCK -
cytarabine system may be a useful approach for gene therapy of
squamous cell carcinomas of the head and neck . -
pubmed
X - ray single - crystal analyses were carried out for
[ Co ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )]( n ) ( ) ( 1 ) , which crystallizes in the triclinic system , space group
P1 macro , with a = 6.477(1 ) A , b = 7.130(1 ) A , c = 9.826(2 ) A , alpha = 108.9(1 ) degrees , beta = 93.97(3 ) degrees , gamma = 98.82(3 ) degrees , and Z = 2 ; for
[ Ni ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )( 2 )]( n ) ( ) ( 2 ) , in the triclinic system , space group
P1 macro , a = 6.425(1 ) A , b = 8.115(2 ) A , c = 10.146(2 ) A , alpha = 113.09(3)(o ) , beta = 99.64(3)(o ) , gamma = 98.90(3)(o ) , and Z = 2 ; and for
[ Zn ( C ( 8 ) NH ( 5 ) O ( 4 ))( H ( 2 ) O )]( n ) ( ) ( 3 ) , in the monoclinic system , space group P2(1)/n , a = 9.044(1 ) A , b = 8.264(1 ) A , c = 11.646(1 ) A , beta = 100.77(1 ) degrees , and Z = 4 . -
pubmed
None of the endothelial cell markers (
thrombomodulin ,
P - selectin ,
E - selectin ,
intracellular adhesion molecule ,
vascular cell adhesion molecule ) ,
thrombin and
plasmin generation markers (
thrombin -
antithrombin complexes ,
prothrombin fragments 1 + 2 ,
plasmin - antiplasmin complexes , D - dimers ) ,
protein C ,
protein Z ,
vascular endothelial growth factor , and
TAFI concentration and activity were directly correlated with the level of
proteinuria ,
albumin ,
cholesterol ,
triglycerides or
creatinine , except significant positive correlations between
TAFI activity and serum
creatinine ,
E - selectin and
albumin as well as negative correlations between
plasmin - antiplasmin complexes and
proteinuria . -
pubmed
Therapeutic doses of
UH are monitored by the classical coagulation assays , activated partial
thromboplastin time ( aPTT ) and
thrombin time ( TT ) , but also by automated chromogenic tests of
anti - factor Xa activity . -
pubmed
Phenotype , cytotoxic activity and
cytokine production of these clones were analyzed by flow cytometry ,
51Cr release assay and ELISA , respectively . -
pubmed
The expression profiles of the
AHP CEI12 at different
IPTG concentrations and different inducing times demonstrated no significant differences by
SDS - PAGE analysis . -
pubmed
OBJECTIVE : A prospective double - blind randomized study undertaken to assess the effect of postoperative prophylactic " renal - dose "
dopamine on
post - coronary artery bypass grafting surgery 's clinical outcome . -
pubmed
The mean pH was higher in group D at 8 hours after operation ( 7.38 + /- 0.2 vs 7.36 + /- 0.3 , p =
NS ) , due to higher
bicarbonate levels ( 23 + /- 2 mmol / l vs 21 + /- 2 , p = 0.49 ) . -
pubmed
The identification of five
coumarins and eleven
daucane derivatives by HPLC - diode array detection ( HPLC - DAD ) and HPLC -
MS is described . -
pubmed
CONCLUSIONS : Both physician and patient - rated scales indicate that
fluvoxamine CR is effective and safe for the treatment of generalized
social anxiety disorder . -
pubmed
Less frequent are patients with an obstruction in the outflow tract of the heart or disorders in binding
oxygen to
hemoglobin . -
pubmed
3 - Hydroxy-3 - methylglutaryl coenzyme A reductase inhibitors ( statins ) are potent inhibitors of
cholesterol biosynthesis . -
pubmed
With respect to these effects , statins augment the expression of
bone morphogenetic protein-2 , a potent simulator of osteoblast differentiation and its activity , and promote mineralization by cultured osteoblasts , indicating that
statins have an anabolic effect on bone . -
pubmed
In the
succinate experiments the mitochondria were contracted in buffered
KCl layered over
sucrose in centrifuge tubes and centrifuged down through distinct
wash , reactive and isotope exchange layers . -
pubmed
The stability of
CaX approximately I must be low for the mitochondria swell very rapidly upon exhaustion of
NADH or blocking of
succinate oxidation by
cyanide . -
pubmed
The subjects were initially screened by an enzyme immunoassay whereas Western blot , INNO - LIA HTLV ,
polymerase chain reaction and
nucleotide sequencing confirmed the
infection . -
pubmed
We report herein the results of fully atomistic molecular dynamics ( MD ) simulations of a series of Gemini surfactants :
CsH2s - alpha , omega - bis ( C12H25N +( CH3 ) 2Cl - ) , at the air / water interface with s = 3 , 4 , 6 , 12 , 14 , and 16 , at values of the initial surface area per surfactant AS = 70
A2 , 77
A2 , 95
A2 , 151
A2 , 133
A2 , and 103
A2 , respectively . -
pubmed
The
Rho family of GTPases regulates many aspects of cellular behavior through alterations to the
actin cytoskeleton , acting as molecular switches cycling between the active ,
GTP - bound and the inactive ,
GDP - bound conformations . -
pubmed
RhoE protein levels increase upon exposure to UVB , and ablation of
RhoE induction through small interfering RNA resulted in a significant increase in apoptosis and a reduction in the levels of the pro - survival targets
p21 ,
Cox-2 , and
cyclin D1 , as well as an increase of reactive
oxygen species levels when compared with control cells . -
pubmed
After the
GST -
HER2 ECD inclusion bodies were solubilized with denaturation buffer containing 8 M
urea , an approach was then used to optimize refolding parameters . -
pubmed
Comparison of
apolipoprotein B and
non - high - density lipoprotein cholesterol for identifying
coronary artery disease risk based on receiver operating curve analysis . -
pubmed
Whether or non -
high - density lipoprotein cholesterol is equivalent to
apolipoprotein B (
apo B ) for screening remains controversial . -
pubmed
We found that
post - pMCAO injections with
HDAC inhibitors ,
valproic acid (
VPA ) ,
sodium butyrate ( SB ) , or
trichostatin A (
TSA ) , decreased
brain infarct volume . -
pubmed
The reduction in levels of acetylated
histone H3 in the ischemic brain was prevented by treatment with
VPA , SB , or
TSA . -
pubmed
Moreover , injections with
HDAC inhibitors superinduced
heat - shock protein 70 and blocked pMCAO - induced down - regulation of
phospho -
Akt , as well as
ischemia - elicited up - regulation of
p53 ,
inducible nitric oxide synthase , and
cyclooxygenase-2 . -
pubmed
After 3 months '
atorvastatin therapy , serum levels of total
cholesterol (
TC ) ,
low - density lipoprotein cholesterol (
LDL - C ) and
coenzyme Q10 ( 0.43 + /- 0.23 to 0.16 + /- 0.10 microg / mL ) were all significantly reduced ( all p<0.001 ) . -
pubmed
On day 2 after the last
atorvastatin , the
coenzyme Q10 level was significantly elevated ( 0.37 + /- 0.16 microg / mL ) and maintained the same levels on day 3 ( 0.39 + /- 0.18 microg / mL ) compared with those on month 3 ( both p< ; 0.001 ) , while
TC and
LDL - C did not significantly change within the same 3 days . -
pubmed
These
prostanoids and their
deuterium derivatives , which were used as internal standards , were subjected to solid - phase extraction using Empore
C18 HD disk cartridges and analyzed in the selected reaction - monitoring mode . -
pubmed
In contrast , none of the
PPAR - gamma agonists tested ( 0.1 , 1 and 10 microM ) altered the basal and the
insulin ( 0.1 microM)-induced
[ ( 3 ) H ]- glucose uptake into adipocytes of both species . -
pubmed
Novelty seeking was not associated with differences in
5HIAA ,
HVA ,
MHPG , or
CRF , but correlated with differences in object exploration observed in a different test situation at an earlier age . -
pubmed
PURPOSE :
Ispinesib , a kinesin spindle protein inhibitor , blocks assembly of a functional mitotic spindle , leading to
G2 / M arrest . -
pubmed
Docetaxel promotes
tubulin assembly into microtubules while inhibiting microtubule de - polymerization leading to mitotic arrest . -
pubmed
The goal was to fit a Bayesian population PK / PD model to characterize the relationship between the
ispinesib /
docetaxel combination and absolute neutrophil counts (
ANC ) . -
pubmed
The PK properties of
ispinesib and
docetaxel , and the relationship of PK with
ANC were investigated using nonlinear mixed - effects models and Bayesian methods . -
pubmed
This
ANC model has been used previously for
ispinesib given as monotherapy , and for other chemotherapeutic drugs in the literature . -
pubmed
An electrophysiological analysis of heterologous expression system exhibited that R468Q -
CACNB4 showed greater
Ba ( 2 + ) current density compared with the wild - type
CACNB4 . -
pubmed
The greater
Ca(v)2.1 currents caused by the R468Q -
CACNB4 mutation may increase the neurotransmitter release in the excitatory neurons under the condition of insufficient inhibitory neurons caused primarily by the
SCN1A mutation . -
pubmed
Previously , a series of structurally related novel
7 - membered iminocyclitols were synthesised and found to be inhibitors of
alpha - mannosidase using in vitro assays . -
pubmed
The present study aimed to delineate
alpha - mannosidases hydrolytic pathways in
azepane inhibitor treated cells by the analysis of free
oligosaccharides (
FOS ) as markers of endoplasmic reticulum (
ER ) , Golgi , lysosomal and cytosolic
alpha - mannosidase activities . -
pubmed
Specific
ER - mannosidase inhibition by
kifunensine also reveals significant non - proteasomal degradation following
FOS analysis and appears to be cell line dependent . -
pubmed
After induction therapy , remission was maintained with low - dose
prednisone and
azathioprine for 4.5 years despite high levels of anti -
MPO antibodies ( & gt;100 U / mL ) . -
pubmed
During the postadolescent period ( 10 - 14 weeks postnatal ) , immunohistochemical experiments were carried out to investigate
c - Fos expression following the administration of
R -(+)- 8 - hydroxy-2 -( di - n - propylamino ) tetralin (
8 - OH - DPAT ) , a
5 - HT ( 1A ) receptor agonist . -
pubmed
In the 3wFS group , the
8 - OH - DPAT - induced
c - Fos expression in the medial prefrontal cortex was significantly attenuated compared to that in the non - FS control group . -
pubmed
A recent report in the New England Journal of Medicine on the early clinical evaluation of
Olaparib in
cancers lacking the
BRCA1 or
BRCA2 genes exemplifies this promising new trend . -
pubmed
KIT alterations have been identified in
melanoma and treatment with
imatinib has met with some success . -
pubmed
The test - retest reliability of the
SDS was found to be very good ( the ICC for all the centres was 0.76 ; overall kappa value for
SDS questions was 0.58 ; one - third of
SDS questions had a kappa value of 0.60 or higher ) . -
pubmed
Her full blood count ,
urea ,
electrolytes ,
uric acid ,
calcium ,
phosphate ,
alkaline phosphatase , and thyroid function levels were all within normal ranges , and her serum was negative for
rheumatoid factor . -
pubmed
Carbonyl reductase was immobilized on
silica with an optimized protein loading of 4 mg / g support . -
pubmed
Parotid constraints were a mean dose to either parotid & lt ; 26 Gy (
PP1 ) , 50 % of either parotid & lt ; 30 Gy (
PP2 ) , or 20 cc of total parotid & lt ; 20 Gy ( PP3 ) . -
pubmed
PP2 ( parotid constraint of 30 Gy to less than 50 % of one parotid ) was the easiest to achieve ( group I ,
II , and III : 82 % , 76 % , and 78 % , respectively ) . -
pubmed
Among these cycle - arrested cells , the proportion of cells in M phase was down - regulated after
OTA treatment by the mitotic index and the level of
phospho - histone H3 . -
pubmed
The
cyclinB1 -
Cdc2 complex was reduced and the expression of
Cdc25C ,
Cdc2 and
cyclinB1 were significantly decreased by
OTA treatment both at protein and mRNA level , respectively . -
pubmed
In conclusion , cell apoptosis and G(2 ) phase arrest mediated by
Cdc25C ,
Cdc2 and
cyclinB1 may be the initiating event in the
gastric toxicity of
OTA . -
pubmed
However , the substitution mutation
mlt-10(mg364 ) , which disrupts the
proline - rich repeats , causes the most severe phenotype . -
pubmed
The
neurological impairment was regressive within 6 months after intravenous
immunoglobulin treatment followed by intravenous then oral
corticosteroid administration . -
pubmed
High - dose
clopidogrel significantly reduced
OTR from 285 + - 47
P2Y(12 ) reaction units ( PRU ) to 220 + - 91 PRU ( p & lt ; 0.001 ) . -
pubmed
The structures of the self - assembled monolayers of various
4 - alkoxybenzoic acids physisorbed at the liquid - solid interface were established by employing scanning tunnelling microscopy (
STM ) . -
pubmed
This solvent - induced convergence of supramolecular patterns is attained by means of co - crystallisation of
octanoic acid molecules in the 2D crystal lattice , which is evident from high - resolution
STM images . -
pubmed
Analyzing together all the data , taking into account the plasma
melatonin concentration versus the expression of
adhesion molecules or
iNOS we detected a significant inverse correlation . -
pubmed
Caspase inhibitors altered the effects of regression induced by
peroxide or
vitamin C. -
pubmed
At the same time ,
caspase inhibitors had little effect on reactive
nitrogen species that are also associated with regression . -
pubmed
Lowered expression of heparan
sulfate / heparin biosynthesis enzyme
N - deacetylase / n - sulfotransferase 1 results in increased sulfation of mast cell heparin . -
pubmed
Surprisingly , the relative amount of heparin produced in
NDST1(+/- ) and
NDST1(-/- ) MCs is higher ( 30 % ) than in control MCs where 95 % of the
( 35 ) S - labeled glycosaminoglycans produced is
chondroitin sulfate . -
pubmed
Lowered expression of
NDST1 also results in a higher
sulfate content of the heparin synthesized and is accompanied by increased levels of stored MC
proteases . -
pubmed
Carbonic anhydrase inhibitors : in vitro inhibition of alpha isoforms (
hCA I ,
hCA II ,
bCA III ,
hCA IV ) by
flavonoids . -
pubmed
A series of
flavonoids , such as
quercetin ,
catechin ,
apigenin ,
luteolin ,
morin , were investigated for their inhibitory effects against the
metalloenzyme carbonic anhydrase (
CA ) . -
pubmed
The
flavonoids inhibited
hCA I with K(I)-s in the range of 2.2 - 12.8 muM ,
hCA II with K(I)-s in the range of 0.74 - 6.2 muM ,
bCA III with K(I)-s in the range of 2.2 - 21.3 muM , and
hCA IV with inhibition constants in the range of 4.4 - 15.7 , with an
esterase assay using
4 - nitrophenyl acetate as substrate . -
pubmed
( 2 ) Activation of cell - mediated immune pathways as indicated by an increased production of
interferon gamma and
neopterin . -
pubmed
( 4 ) Lowered levels of key antioxidants , such as
coenzyme Q10 ,
zinc ,
vitamin E ,
glutathione , and
glutathione peroxidase . -
pubmed
using cytokine antagonists ,
minocycline ,
Cox-2 inhibitors , statins ,
acetylsalicylic acid ,
ketamine ,
omega3 poly - unsaturated fatty acids , antioxidants , and
neurotrophic factors . -
pubmed
Pharmacological
LXR activation reduces presence of
SR - B1 in liver membranes contributing to
LXR - mediated induction of HDL -
cholesterol . -
pubmed
Part of these beneficial effects may be explained by accelerated reverse
cholesterol transport since both plasma high density lipoprotein ( HDL )
cholesterol and fecal neutral
sterol secretion are higher upon
LXR activation . -
pubmed
RESULTS :
HDL cholesterol was maximally 60 % increased in a time - dependent fashion due to appearance of more and larger
HDL particles . -
pubmed
Surprisingly ,
LXR activation reduced
SR - B1 protein amount in hepatic membranes , suggesting that this might contribute to elevated HDL
cholesterol . -
pubmed
In addition ,
SR - B1 is not essential for
LXR - induced
cholesterol removal from the body . -
pubmed
CONCLUSION : Induction of fecal neutral
sterol secretion by
T0901317 critically depends on
LXRalpha but not on
LXRbeta . -
pubmed
LXR activation reduces
SR - B1 in hepatic membranes , probably partly contributing to elevated HDL
cholesterol . -
pubmed
SR - B1 is not required to enhance fecal neutral
sterol secretion . -
pubmed
present the structure of an extracellular undecaheme
cytochrome involved in the step of electron transfer to
metal oxides . -
pubmed
MetS in children is based on the presence of any three of the following : waist circumference & gt;= 90th percentile for age and sex ; plasma
triglyceride & gt;= 1.24 mmol l(-1 ) ; plasma
high - density lipoprotein -
cholesterol & lt;= 1.03 mmol l(-1 ) ; systolic or diastolic blood pressure & gt;= 90th percentile for age , sex , and height ; and fasting
glucose & gt;= 5.6 mmol l(-1 ) . -
pubmed
Models adjusted for duration of SGA treatment , ethnicity , sex , age and use of other medications revealed a positive relationship between the
MTHFR 677 T allele and diastolic blood pressure Z - scores ( P=0.001 ) and fasting plasma
glucose ( P<0.05 ) in SGA - treated children . -
pubmed
The aim of the study was to determine the influence of treatment with
heparin on protective effect of ischemic preconditioning (
IP ) in
ischemia / reperfusion - induced acute
pancreatitis . -
pubmed
Pretreatment with
heparin or
IP applied alone reduced the severity of acute
pancreatitis . -
pubmed
Combination of
heparin plus
IP reduced the protective effect of
heparin or IP applied alone . -
pubmed
The unsaturated
C-6 ' linoleic acid ester derivative 14 that was structurally most similar to 1 and 2 , inhibited
HIF-1 activation with comparable potency ( IC50 0.92 muM ) . -
pubmed
The
octanoyl ( 11 ) and
undecanoyl ( 12 ) ester derivatives also suppressed
HIF-1 activation ( IC50 values 3.1 and 0.87 muM , respectively ) . -
pubmed
One surprising observation was that , while less potent , the purported
cancer chemopreventive agent
auraptene ( 8) was found to act as a mitochondrial poison that disrupts
HIF-1 signaling in
tumors . -
pubmed
obtusiloba inhibit mast - cell - derived
allergic inflammation ,
histamine , and proinflammatory
cytokines . -
pubmed
In summary , direct phosphorylation of the A subunit at
Ser-384 by
AMPK represents a novel regulatory mechanism of the
V - ATPase in kidney intercalated cells . -
pubmed
With
sulfadimethoxine and
thrombin as model analytes , we found that water resuspended AuNPs can significantly increase the sensitivity by more than 10 - fold for AuNPs based fluorescence quenching aptasensor . -
pubmed
Design and synthesis of novel
pyrimidone analogues as
HIV-1 integrase inhibitors . -
pubmed
A series of novel
pyrimidone analogues have been designed and synthesized as
HIV-1 integrase (
IN ) inhibitors . -
pubmed
This study demonstrated that introducing a substituent in the N1 - position of the
pyrimidone scaffold does not significantly influence
IN inhibitory activity . -
pubmed
The
carboxy - terminal segment of the
human LINE-1 ORF2 protein is involved in RNA binding . -
pubmed
Here , we explore the nucleic acid binding properties of the 180 -
amino acid carboxy - terminal segment ( CTS ) of the
human L1 ORF2p in vitro . -
pubmed
Finally , we report that mutations destroying the putative
Zn - knuckle structure of the protein do not significantly affect the level of RNA binding and discuss the possible functional role of the CTS in
L1 retrotransposition . -
pubmed
BACKGROUND : A combination of inhaled
corticosteroid and long - acting
beta2 agonist ( ICS / LABA ) is used frequently to treat
chronic obstructive pulmonary disease (
COPD ) patients . -
pubmed
[ Associations of
ulcerative colitis with
vitamin D receptor gene polymorphisms and serum levels of
25 - hydroxyl vitamin D ] . -
pubmed
OBJECTIVE : To explore the associations of
ulcerative colitis (
UC ) with
vitamin D receptor (
VDR ) gene polymorphisms and serum levels of
25 - hydroxyl vitamin D[
25 ( OH ) D ] . -
pubmed
Four single
nucleotide polymorphisms of
VDR (
Fok I ,
Bsm I ,
Apa I ,
Taq I ) were detected by mini - sequencing technique . -
pubmed
By linear regression analysis , the serum levels of
25 ( OH ) D in
UC patients were independently and positively correlated with
hemoglobin ( beta = 0.499 , P & lt ; 0.01 ) and yet independently and negatively correlated with
C - reaction protein ( beta = -0.346 , P & lt ; 0.01 ) and white blood cells ( beta = -0.291 , P = 0.002 ) . -
pubmed
CONCLUSIONS : Genetic polymorphism of
VDR (
Fok I ,
Bsm I ,
Apa I ,
Taq I ) and serum levels of
25 ( OH ) D are significantly correlated with
UC . -
pubmed
QYHJ upregulated the mRNA and protein level of
Eph receptor - interacting B1 (
EphrinB1 ) in the cells that were expressing different levels of
EphB2 , however , QYHJ did not regulate
EphB2 expression . -
pubmed
Mean total
testosterone ,
LH , and
FSH were 6.2 + - 1.7 nmol / L , 3.4 + - 2 and 4.7 + - 3.1 mIU / L , respectively . -
pubmed
The photodegradation of
methylene blue by
CdS photocatalyst confined inside
VLP can be significantly enhanced by the controlled deposition of gold nanoparticles on the outer shell of
VLP -
CdS. -
pubmed
CONCLUSION : Within the limitations of this study , dimensional changes of
acrylic resin specimens were influenced by the molding technique used and
SR - Ivocap injection procedure exhibited higher dimensional accuracy compared to conventional molding . -
pubmed
The
leukotriene B4 ( LTB4 ) receptor 1 (
BLT1 ) is a high affinity receptor for
LTB4 , a chemotactic and inflammatory
eicosanoid . -
pubmed
The
LTB4 receptor 2 (
BLT2 ) was originally identified as a low affinity receptor for
LTB4 , and , more recently , as a high affinity receptor for
12 - hydroxyheptadecatrienoic acid (
12 - HHT ) . -
pubmed
Consistently , knockdown of
lta4h , an enzyme mediating
LTB4 production , induced a phenotype similar to knockdown of
blt1 . -
pubmed
Histone deacetylases (
HDACs ) are enzymes that catalyze the removal of
acetyl groups from the
epsilon - amino groups of conserved
lysine residues in the
amino terminal tail of
histones . -
pubmed
The mechanism is poorly understood ; it is unclear whether
phosphate has direct effects or effects mediated via calcification or
FGF23 . -
pubmed
Oxcarbazepine (
Na channel blocker ) and
pregabalin (
calcium channel alpha2 - delta ligand medication ) were tried . -
pubmed
The disease is characterized by type 1
hypersensitivity to fungal allergens , dramatically elevated total serum
IgE , accumulation of thick eosinophil - laden mucin with non - invasive fungal hyphae within the paranasal
sinuses ,
nasal polyposis , and
sinus bony remodeling . -
pubmed
The inferred
amino acid sequence revealed that the putative protein possesses the main structural characteristics of the
Na + K +- ATPase alpha - subunits . -
pubmed
Desensitization Using
Bortezomib and High - dose
Immunoglobulin Increases Rate of Deceased Donor Kidney Transplantation . -
pubmed
Role of Serum
Follicle Stimulating Hormone ,
Luteinizing Hormone ,
Testosterone and
Prolactin Levels in Azoospermic Male Partner of Subfertile Couple . -
pubmed
Using standard ELISA technique , serum levels of
follicle stimulating hormone (
FSH ) ,
luteinizing hormone (
LH ) ,
testosterone and
prolactin were measured / assayed / estimated . -
pubmed
Low
testosterone level ( & lt;270ng / dl ) was observed in 11(13.6 % ) , low
TSH ( & lt;0.4muIU / ml ) in 1(1.2 % ) and low
prolactin ( & lt;2.5ng / dl ) in 5(6.2 % ) . -
pubmed
CD melting experiments demonstrate that
spiroketal does not affect the G - quadruplex (
G4 ) thermal stability . -
pubmed
Compared with the cells in which RNAi of the
CAS gene was achieved , the cells with simultaneously over - expressed
FPS and silenced
CAS showed higher
triterpene contents . -
pubmed
These results demonstrate that over - expression of
FPS can break the rate - limiting reaction catalyzed by
FPS in the
triterpene saponins biosynthetic pathway ; and inhibition of
CAS expression can decrease the synthesis metabolic flux of the
phytosterol branch . -
pubmed
The subanesthesia dose
sevoflurane in 60 %
oxygen may reduce
sepsis - induced inflammatory responses in animals and in PBMCs , and the inhibition to the activation of the
NF - kappaB pathway may contribute to this protection . -
pubmed
The aim of this study was to investigate
epidermal growth factor receptor (
EGFR ) mutation status in
lung adenocarcinoma - associated
MPEs ( LA -
MPEs ) and its correlation with efficacy of
EGFR tyrosine kinase inhibitor ( TKI ) therapy . -
pubmed
The full - length cDNA sequence of
L - LECT2 consists of a 606 - bp ORF encoding a protein of 201
amino acid residues . -
pubmed
The low
mGluR5 protein group (
LE ) showed increased methylation of the
mGluR5 CpG island , reduced
mGluR5 mRNA levels , and unaltered basal EEG theta spectral power between stress day 1 and 6 . -
pubmed
In contrast , the high
mGluR5 protein group (
HE ) showed reduced methylation of
CpG sites , increased
mGluR5 mRNA expression , and reduced basal theta spectral power on stress day 6 . -
pubmed
Nitrogen - doped graphene oxide sheets ( N - GOs ) are prepared by employing N - containing polymers such as
polypyrrole ,
polyaniline , and copolymer (
polypyrrole -
polyaniline ) doped with acids such as
HCl ,
H2SO4 , and
C6H5 - SO3 - K , which are activated using different concentrations of
KOH and carbonized at 650 C ; characterized using SEM ,
TEM , BET ,
TGA - DSC , XRD , and XPS ; and employed for the removal of environmental pollutant
CO2 . -
pubmed
Furthermore ,
IrM2 rapidly induces a series of mitochondria - related dysfunctional events , including the loss of mitochondrial membrane potential , cellular
ATP depletion , mitochondrial respiration inhibition and reactive
oxygen species ( ROS ) elevation . -
pubmed
Among the ROS ,
superoxide anion radicals play a critical role in
IrM2 - mediated cell
death . -
pubmed
Preparation of Complexes between
Ovalbumin Nanoparticles and
Retinoic Acid for Efficient Induction of Tolerogenic Dendritic Cells . -
pubmed
We have proposed a simple formulation of
ovalbumin nanoparticles complexed with
ATRA ( OVA / RA NPs ) . -
pubmed
This protocol successfully avoids the isolation , purification and storage of carcinogenic
isocyanates and allows elaboration of
urethanes and
ureas in a one -
pot process from commercially available sources . -
pubmed
A mechanistic study verified that
nanosilica induced autophagy via negative regulation of
mammalian target of rapamycin (
mTOR ) signaling but not the
Beclin-1 associated pathway . -
pubmed
Additionally , production of
lymphoid chemokines and
cytokines associated with ectopic lymphoneogenesis and , remarkably , saliva flow and autoantibody production , were significantly affected by treatment with
seletalisib . -
pubmed
Interest has been expanded to areas not only limited to low - density lipoprotein
cholesterol reduction but also investigating other non - lipid pathways raising cardiovascular risk , in particular
inflammation associated to raised high - sensitivity
C - reactive protein levels , not significantly affected by the present
PCSK9 antagonists . -
pubmed
In this study , chitosan (
CS ) loading well - dispersed nanoscale zero - valent
iron (
NZVI /
CS ) was successfully prepared via the liquid - phase reduction method . -
pubmed
Characterizations of the
NZVI /
CS with high - resolution transmission electron microscopy and X - ray diffraction suggested that the as - prepared
NZVI /
CS comprised numerous dispersed
Fe0 nanoparticles . -
pubmed
These findings indicate that the synthesized
NZVI /
CS composites have potential application for the removal of
U ( VI ) from the sewage water . -
pubmed
Mean Urine
albumin - to -
creatinine ratio ( UACR ) was 0.02 , and 166 subjects showed significant
albuminuria ( UACR & gt;= 0.03 ) . -
pubmed
The EQE of an
OPD with
P3HT :
DRCN5 T ( 100 : 2 , wt / wt ) as the active layer reaches 1400 % at -10 V , exhibiting the photomultiplication ( PM ) phenomenon . -
pubmed
The
macrosomia rate was higher in the
glibenclamide - exposed group than the
insulin - alone group ( 12.2 % vs 0 % , P=0.025 ) . -
pubmed
The
Na - K - Cl cotransporter-1 (
NKCC1 ) , by mediating the electroneutral transport of
Na+ ,
K + , and
Cl- plays an important role in cell volume regulation , epithelial transport , and the control of neuronal excitability . -
pubmed
Studies using
catalase mutants have revealed that the inducible and constitutive elevations of intracellular
hydrogen peroxide (
H2O2 ) have different roles : only the inducible
H2O2 production transduces the
abscisic acid (
ABA ) signal leading stomatal closure . -
pubmed
We studied
H2O2 and
NO mobilization in guard cells of
catalase mutants . -
pubmed
Abbreviations :
ABA :
abscisic acid ;
CAT :
catalase ;
cGMP :
cyclic guanosine monophosphate ;
DAF-2DA :
4,5 - diaminofluorescein-2 diacetate ;
H2DCF - DA :
2 ' , 7 '- dichlorodihydrofluorescein diacetate ;
MeJA :
methyljasmonate ;
NOS :
nitric oxide synthetase ; NR :
nitrate reductase ;
POX :
peroxidase ; ROS : reactive
oxygen species ; SNAP :
S - nitroso - N - acetyl - DL - penicillamine ;
SNP :
sodium nitroprusside ;
NOX : NADP ( H ) oxidase . -
pubmed
Meta - analysis showed that the changed
FMD value before and after treatment with
nebivolol was not statistically different from those treated with other beta - blockers [ Mean difference=1.12 , 95 % CI ( -0.56,2.81 ) , P=0.19 ] . -
pubmed
Moreover , the correlations of
ACE2 with
CS - induced oxidative stress - related markers ,
hypoxia inducible factor-1alpha (
HIF-1alpha ) ,
inducible nitric oxide synthase (
iNOS ) , and
4 - hydroxynonenal (
4 - HNE ) were investigated . -
pubmed
The modification of HA on
Pd@
Pt - GOx could block the GOx activity ,
catalase (
CAT)-like and
peroxidase (
POD)-like activities of
Pd@
Pt , reduce the
cytotoxicity to normal cells and organs , and effectively target
CD44 - overexpressed
tumors by active targeting and passive enhanced permeability and retention (
EPR ) effect . -
pubmed
After endocytosis by
tumor cells , the intracellular
hyaluronidase (
Hyase ) could decompose the outer HA and expose
Pd@
Pt - GOx for the enzymatic cascade reaction . -
pubmed
Meanwhile , the upregulated acidity and
H2O2 content in the
tumor region generated by GOx catalytic oxidation of
glucose dramatically facilitated the pH - responsive
POD - like activity of the
Pd@
Pt nanozyme , which then catalyzed degradation of the
H2O2 to generate abundant highly toxic
OH , thereby realizing nanozyme - mediated starving - enhanced chemodynamic
cancer therapy . -
pubmed
Molecular subtype - specific responses of
colon cancer cells to the
SMAC mimetic
Birinapant . -
pubmed
In this study , responses to the
IAP antagonist
Birinapant and
oxaliplatin/
5 - fluorouracil (
5 - FU ) were investigated in 14
colon cancer cell lines , representing the consensus molecular subtypes (
CMS ) . -
pubmed
We observed increased cell
death during
Birinapant single treatment in these co - cultures , which was abrogated by anti -
TNFalpha - neutralizing antibodies . -
pubmed
Collectively , our study demonstrates that
IAP inhibition is a promising modulator of response to
oxaliplatin/
5 - FU in
colorectal cancers of the CMS1 subtype , and may show promise as in the CMS2 subtype , suggesting that molecular subtyping may aid as a patient stratification tool for
IAP antagonists in this disease . -
pubmed
Amid those , some bind to yet untapped
amino - acids in the
S1 pocket . -
pubmed
Cisternography and
CT scanning with
111In - DTPA in evaluation of
posterior fossa arachnoid cysts . -
pubmed
Mechanism of action of
adenosylcobalamin :
hydrogen transfer in the inactivation of
diol dehydratase by
glycerol . -
pubmed
We have investigated the kinetic characteristics of the inactivation of the
adenosylcobalamin - dependent enzyme propanediol dehydratase by
glycerol ,
( RS )- 1,1 - dideuterioglycerol ,
( R )- 1,1 - dideuterioglycerol , and
perdeuterioglycerol in the presence of
1,2 - propanediol and
1,1 - dideuterio-1,2 - propanediol . -
pubmed
When
diol dehydratase holoenzyme is inactivated by
[ 1 - 3H ] glycerol ,
5 '- deoxyadenosine which is enriched in
tritium by a factor of 2.1 over that in
glycerol can be isolated from the reaction mixture . -
pubmed
Disease specific accumulation of
PrP was observed at light microscopy as
amyloid plaques or as diffuse or granular staining within the neuropil , often clearly associated with individual neurons . -
pubmed
Serial
electron microscopical preparations were immunostained for
PrP by the immunogold method . -
pubmed
This suggests that
amyloid fibrils are formed following the accumulation and aggregation of sub - unit proteins at the plasmalemma and , furthermore , that normal
PrP may be converted to its pathological form at this site . -
pubmed
Moreover , in these clones
H-2Kb expression could be enhanced in the presence of
Zn2 + , indicating that the metallothionein enhancer was functioning properly . -
pubmed
It is concluded that those with frequent occupational exposure to M
tuberculosis have larger numbers of circulating T cells reactive with mycobacterial
antigens , so that the development of the skin test response to
tuberculin is less dependent on " by - stander " cell infiltration to mediate the delayed
hypersensitivity reaction than the reactions in those with less intense and less frequent natural exposure . -
pubmed
These findings were consistent with the hypothesis that
metrizamide is a competitive inhibitor of
human brain hexokinase . -
pubmed
The maximum change in polymorphonuclear leukocytes ( PMNs ) was detected 6 h after
O3 , and the maximum increase in
BAL protein occurred 24 h postexposure . -
pubmed
The phenotypes of the C57BL/6J ( B6 , termed susceptible ) and C3H / HeJ (
C3 , termed resistant )
strains were easily distinguished by the magnitude of their inflammatory responses to
O3 . -
pubmed
The treatment with the 5 mg dose had minor and inconsistent effect on the motility ,
hyaluronidase and
acrosin activity , and the fertilizing ability of spermatozoa in addition to the blood level of
testosterone . -
pubmed
The role of
histamine in
cardiac anaphylaxis ; characterization of
histaminergic H1 - and H2 - receptor effects . -
pubmed
It is concluded that
histamine might be an important mediator of these effects , since in the presence of
H2 - receptor blockade with
cimetidine ( 6.2 x 10(-7 ) moles / min ) , the positive inotropic and chronotropic effects were completely antagonized . -
pubmed
The
O2 reducing ability of the organism is due to the presence in the cells of a particle - bound
NADH oxidase , a soluble
NADPH oxidase and a soluble
pyruvate oxidase . -
pubmed
The two
pyridine nucleotide oxidases reduce
O2 to
H2O2 , the
pyruvate oxidase reduces
O2 to
H2O. -
pubmed
Kinetic studies with cell - free extracts showed that the
pyruvate oxidase had a considerably greater affinity ( smaller Km ) for
O2 and capacity ( higher Vmax ) for
O2 reduction than the two other
oxidases . -
pubmed
It is postulated that the
pyruvate oxidase acts as a scavenger for
O2 , leading to the non - toxic reduction product
H2O , and thus functions as a defense mechanism against
oxygen toxicity when the organism is exposed to aerobic condition . -
pubmed
The effect of various drugs affecting the dopaminergic system in the brain were also investigated to clarify the relationship between
NT and
dopamine . -
pubmed
Intraperitoneal(ip ) bolus administration of
L - DOPA caused the reduction in NTLI concentration from 9.2 + /- 1.0 ng / g wet wt to 5.3 + /- 2.1 ng / g wet wt and Bmax of
NT receptor from 19.7 + /- 3.1 fmol / mg protein to 13.9 + /- 2.6 fmol / mg protein with no change in Kd . -
pubmed
Intracerebroventricular administration of
dopamine also induced a significant reduction of NTLI concentration and Bmax of
NT receptor with no change in Kd . -
pubmed
Inversely , ip bolus administration of
alpha - methyldopa evoked an increase in NTLI concentration from 6.9 + /- 0.9 ng / g wet wt to 12.0 + /- 3.0 ng / g wet wt and that in Bmax of
NT receptor from 15.9 + /- 2.9 fmol / mg protein to 24.2 + /- 4.6 fmol / mg protein . -
pubmed
Serial administration of
haloperidol evoked an increase in Bmax of
NT receptor from 16.9 + /- 1.5 fmol / mg protein to 20.5 + /- 1.9 fmol / mg protein with no changes in Kd and NTLI concentration . -
pubmed
Like the
engrailed gene , the invected gene can encode a protein of approximately 60 kD that contains a homeo box near its carboxyl terminus ; indeed , a sequence of 117
amino acids in the carboxy - terminal region of both proteins is almost identical . -
pubmed
The induction of
cytochrome P-450 by
isosafrole and related
methylenedioxyphenyl compounds . -
pubmed
Isosafrole ,
piperonyl butoxide , and
5 - t - butyl-1,3 - benzodioxole were unable to displace
2,3,7,8 - tetrachlorodibenzo - p - dioxin or
3 - methylcholanthrene from either the
Ah receptor or the
3 - 4S binding peak , in vitro . -
pubmed
injection ; whereas ,
isosafrole caused a 2 - fold enhancement of the
Ah receptor after 24 h. This increase in the
Ah receptor binding following
isosafrole treatment may be due to induction . -
pubmed
Both in vivo and in vitro data appear to demonstrate that there is no direct role for the
Ah receptor or the
3 - 4S protein in the regulation of
cytochrome P-450 by
methylenedioxyphenyl compounds . -
pubmed
We examined the production of
oxygen radicals by synovial fluid PMNs (
SF - PMNs ) and peripheral blood PMNs (
PB - PMNs ) by measuring chemiluminescence ( CL ) as well as
superoxide anion (
O2- ) release . -
pubmed
Increased spontaneous CL in the presence of
luminol and increased CL in response to
phorbol myristate acetate (
PMA ) was observed in
SF - PMNs when compared to
PB - PMNs . -
pubmed
When zymosan was used as the stimulus in the absence of
luminol , a slightly lower CL response was observed in
SF - PMNs as compared to
PB - PMNs . -
pubmed
When
O2- release from normal
PB - PMNs pretreated with 10 % synovial fluid was compared to untreated controls , enhancement of spontaneous
O2- release was observed . -
pubmed
Increased spontaneous and
PMA - stimulated release of
myeloperoxidase (
MPO ) was also observed in normal
PB - PMNs pretreated with synovial fluid . -
pubmed
These findings may explain the increased
luminol - dependent CL since this type of CL requires the presence of
MPO . -
pubmed
Our findings suggest that the enhanced chemiluminescence observed in normal PMNs treated with synovial fluids may be related to increases in spontaneous
O2- generation and
myeloperoxidase release . -
pubmed
A
tumor promoter ,
12 - O - tetradecanoylphorbol-13 - acetate (
TPA ) also augmented anchorage - independent growth of
ras - transformed cells and induced morphological changes in monolayer cultures without altering the expression of the
ras gene or phosphorylation of the
p21ras protein . -
pubmed
Although the total percentages of
PUFA in the two
phosphoglycerides were not altered , compared with controls the ratio of
PUFA of the ( n-3 ) series to those of the ( n-6 ) series was very significantly elevated in
Down ' s syndrome , from 0.32 to 0.55 in
EPG and from 0.60 to 0.97 in
SPG . -
pubmed
In particular ,
docosahexaenoyl , 22:6(n-3 ) , groups were uniformly increased in
Down ' s syndrome compared with controls by 54 % and 33 % in
EPG and
SPG , respectively , while
arachidonoyl , 20:4(n-6 ) , groups were decreased by 16 % and 30 % , respectively . -
pubmed
A control test with a single oral dose of 2.5 mg
bromocriptine was also performed ;
serum PRL and
GH levels were measured at the same time intervals for 2 days . -
pubmed
Cabergoline induced a marked fall in
serum PRL which began within 3 h and continued for 7 days . -
pubmed
31P spectra were obtained using a surface
coil placed into the surgically created pouch and using routine NMR pulsing techniques . -
pubmed
Sedimentation analysis of cell sap in
sucrose density gradients shows a shift of
prolyl - tRNA synthetase activity toward the high - Mr form in regenerating liver . -
pubmed
Under artificial photoperiods ganglionectomy suppressed the stimulatory role of long days and the inhibitory role of short days on
prolactin secretion , and consequently on
progesterone secretion and
spring moult . -
pubmed
However , in animals kept outdoors ,
prolactin and
progesterone secretion and
spring moult were not changed by ganglionectomy . -
pubmed
[ Reduction of
alpha-2 - globulins following administration of
1 - methyl - delta-1 - androstenolone enonthate ] . -
pubmed
The location and identity of different autonomic ( sympathetic and parasympathetic ) and sensory nerves was further studied by
monoamine histofluorescence ,
acetylcholinesterase histochemistry , immunohistochemistry for
substance P and
neurofilament , and Linder 's silver staining . -
pubmed
Ca2 + -dependence of
thrombin - induced aggregation and
MDA formation , and Ionophore
A23187 - induced aggregation of the platelets from SHRSP at a
hypertensive age(16 - weeks ) was similar to that of the aggregation of platelets from age - matched WKY . -
pubmed
The enhancement by
Ca2 + of both
thrombin- and Ionophore
A23187- induced aggregation , however , was markedly less in SHRSP than in WKY , whereas their
MDA formation was equally enhanced by
Ca2 + . -
pubmed
The magnitude of phospholipid(PI , PC ,
PE ) degradation , and
MDA formation IN SHRSP at early- and late-
hypertensive ages(11- and 17 - weeks ) were either the same as or greater than that of age - matched WKY . -
pubmed
In addition ,
thrombin - induced
thromboxane B2 formation in SHRSP platelets was similar to that in WKY in the concentration range of 0.22 - 0.44 U / ml , and became significantly higher at 0.65 U / ml despite severe hypoaggregability of SHRSP platelets in all the concentrations examined . -
pubmed
These results suggest that abnormalities of SHRSP platelets at
hypertensive ages are due to an impaired function of
thromboxane A2 and/or
calcium concerned in aggregation and secretion but not due to a defect in
cyclo - oxygenase and
thromboxane synthetase pathway . -
pubmed
During exercise after
propranolol a limited augmentation in cardiac performance and in myocardial and muscular blood flow was observed ; splanchnic blood flow which was most affected by
propranolol at
rest did not further decrease during exercise after
propranolol . -
pubmed
The influence of
beta - adrenoreceptor blockade was even more evident under conditions of augmented sympathetic activity as reflected by a significant limitation of increase in cardiac performance , myocardial and muscular blood flow and by a sustained redistribution of splanchnic blood flow during exercise after
propranolol . -
pubmed
We developed an improved method for the dye - exclusion
microcytotoxicity test by introducing low melting point
agarose (
LMP - Aga ) into the assay medium . -
pubmed
By contrast , both groups had similar serum levels of
creatinine and
beta 2 - microglobulin . -
pubmed
Concerns about the redox reactivity of cell - free
hemoglobin and its contribution to tissue - damaging
oxygen free radicals has not been fully established . -
pubmed
Superoxide radicals are produced during the spontaneous oxidation of
hemoglobin . -
pubmed
Spectral changes indicative of protein modification and
ferrylhemoglobin formation during the enzymatic peroxidation of these
hemoglobins differ qualitatively and occur at an increasing order , poly HbBv & gt ; HbBv & gt ; HbBv - FMDA . -
pubmed
The proteins also differ in the rate of
hemoglobin catalyzed
NADPH oxidation and
aniline hydroxylation , reactions mediated by reactive
oxygen species . -
pubmed
BACKGROUND : The likelihood of a positive response with
dipyridamole stress echocardiography (
DSE ) is directly related to the extent and severity of angiographically assessed
coronary artery disease . -
pubmed
Antipyrine metabolism depends on at least three isoenzymes of
cytochrome P450 forming the main metabolites
3 - OH - , 4 - OH- and
norantipyrine . -
pubmed
Our results demonstrate that the
cytochrome P450 isoenzymes responsible for the different pathways of
antipyrine metabolism are affected to different extents by
cirrhosis . -
pubmed
In a variety of systems ,
phosphatidylinositol 3 - kinase (
PI 3 - kinase ) is believed to be a downstream effector of
tyrosine kinases , and activation of
PI 3 - kinase results in increased levels of
D3 - phosphorylated metabolites of
phosphatidylinositol (
PtdIns ) . -
pubmed
Increased levels of
PtdIns 3,4,5 - P3 in LPS - treated cells resulted from an increase in the specific activity of
PI 3 - kinase . -
pubmed
Activation of
PI 3 - kinase involved signaling through the
monocyte cell surface molecule CD14 , since pretreatment of cells with
Abs to
CD14 abrogated LPS - induced increases in
PtdIns 3,4,5 - P3 . -
pubmed
Immunoprecipitates of
p53 / 56lyn from LPS - treated cells showed a time - dependent and transient increase in
PI 3 - kinase activity assayed in vitro , coordinate with activation of
p53 / 56lyn , indicating that
LPS induces the association and simultaneous activation of these two enzymes in vivo . -
pubmed
These findings indicate that monocytes respond to LPS with the rapid activation of
PI 3 - kinase , resulting in transient increases in levels of
PtdIns 3,4,5 - P3 . -
pubmed
Outcomes were levels of
high - density lipoprotein cholesterol ,
insulin ( 2 hours after challenge ) ,
fibrinogen ,
systolic blood pressure . -
pubmed
RESULTS : In white women , leisure physical activity was positively associated with levels of
high - density lipoprotein cholesterol ( P = .001 ) and inversely associated with levels of
insulin ( P = .04 ) and
fibrinogen ( P = .02 ) . -
pubmed
Compared with
high - density lipoprotein cholesterol levels in the inactive and light leisure physical activity groups , moderate ( P & lt ; .001 ) and heavy ( P = .004 ) leisure activities were associated with higher
high - density lipoprotein cholesterol levels . -
pubmed
High - density lipoprotein cholesterol levels in the heavy leisure physical activity group were significantly higher than those in the moderate group ( P = .01 ) . -
pubmed
Home physical activity was positively related to
high - density lipoprotein cholesterol level ( P = .01 ) ; relative to lower levels of home physical activity , the heavy home physical activity group demonstrated significantly higher mean
high - density lipoprotein cholesterol levels . -
pubmed
Since there are so few
H + present in the ECF in a free or a bound form , and in the absence of an increase in endogenous acid - production , the source of this
H + is from
proteins in the
ICF . -
pubmed
Accordingly ,
ALA - generated oxyradicals where shown to cause oxidative lesions in biomolecules and isolated cell organelles and to release
iron from
ferritin . -
pubmed
[ Day to day changes in
SAA (
serum amyloid A ) values of healthy subjects and the effect of
dexamethasone administration ] . -
pubmed
Cycloheximide and
actinomycin D inhibited the ability of
depudecin to induce a morphological change , suggesting that both mRNA synthesis and de novo protein synthesis are required for
depudecin - mediated suppression of the transformed phenotypes in
ras - transformed cells . -
pubmed
Estrogen upregulation of
BRCA1 expression with no effect on localization . -
pubmed
The sex - steroid
estrogen modulates cell proliferation of
normal and
neoplastic breast and ovarian epithelial cells , but the role of
estrogen regulation on the expression of
BRCA1 remains to be defined . -
pubmed
In this study ,
estrogen - regulated
BRCA1 expression was examined in
breast and ovarian cancer cells . -
pubmed
Cell fractionation studies localized the
BRCA1 protein to the nucleus in both unstimulated and
estrogen - stimulated cells . -
pubmed
Taken together , these data support a role for the
steroid estrogen and the involvement of the
estrogen receptor pathway in the modulation of expression of
BRCA1 . -
pubmed
Removal of these portions by
trypsin results in a fragment of 40 kDa (
HP40 ) , which lacks 42 NH2 - terminal and 51
COOH - terminal residues . -
pubmed
Methionine aminopeptidase (
MetAP ) , in two isoenzymic forms , is responsible for hydrolysis of the initiator
methionine residues from the majority of newly synthesized proteins . -
pubmed
MetAP also has a potentially important role in the production of recombinant proteins , since failure to correctly remove initiator
methionine residues can result in a product that is inactive or immunogenic . -
pubmed
In contrast with previous methods , which usually use short
tripeptides and require a
post - reaction derivatization step , this assay uses peptides ranging in size from four to eight residues and utilizes UV detection of a
tryptophan residue at the C - terminus . -
pubmed
The
mGluR antagonist
( R , S )- alpha - methyl-4 - carboxyphenylglycine (
MCPG ) was infused into the hippocampus 30 min before fear conditioning , and freezing was measured during both acquisition and retention tests . -
pubmed
This shortening of the
T1 - relaxation time is associated with pallidal deposition of
manganese most likely reflecting the presence of an adaptive process designed to improve the efficacy of
ammonia detoxification by astrocytes . -
pubmed
Partial correction of
anemia with
iron ,
erythropoietin , or
androgens can improve quality of life and potentially prevent
cardiac disease . -
pubmed
In addition to its action at cholinergic synapses
acetylcholinesterase (
AChE ) has been proposed to modulate neuronal activity by mechanisms unrelated to the hydrolysis of
acetylcholine . -
pubmed
This increase is completely blocked by the specific
AChE inhibitors
propidium ,
physostigmine ,
DFP and
BW 284C51 . -
pubmed
AChE pretreatment had no effect on [
3H]
kainate binding . -
pubmed
Furthermore , the
AChE - induced potentiation of [
3H]FW binding is
Ca2 + - and temperature - dependent suggesting an enzymatic action for
AChE in this system . -
pubmed
Saturation binding experiments with [
3H]FW to adult membranes reveal high and low affinity binding sites and demonstrate that the main action of
AChE is to increase the Bmax of both sites . -
pubmed
The objective of this study was to examine the association of
carboxyhemoglobin (
COHb ) and plasma
cotinine levels among pregnant women who smoke cigarettes and their fetuses . -
pubmed
Maternal
cotinine levels were correlated with maternal and fetal
COHb levels ( r = 0.96 , p = 0.003 ; r = 0.99 , p = 0.0003 , respectively ) . -
pubmed
Injections of both
calcium channel blockers --
nifedipine ( 2.5 mg / kg ) and
verapamil ( 5 mg / kg ) did not influence the
ethanol - induced hypnotic activity themselves . -
pubmed
It can be supposed that the modification of the activity of
voltage - dependent calcium channels plays an important role in the anaesthetic action of
ethanol . -
pubmed
RESULTS : It was identified that a missense mutation in exon 6 of
LPL gene ( 6G(979)-->A ) resulted in the substitution of
Glu(242 ) by
Lys in a heterozygous state . -
pubmed
From the results of measurements of the
nucleotide pools extracted from the antisense
strain and its isogenic counterpart , we propose that one of the physiological roles of
SGP is regulation and modulation of the
GTP /
GDP ratio under different growth conditions . -
pubmed
Sensitivity of
bacteriophage RB69 DNA polymerase to
N2 -( p - n - butylphenyl )- 2 '- deoxyguanosine nucleotides . -
pubmed
[
Amiloride - sensitive sodium channel in the nephron and its activation by
vasopressin ] . -
pubmed
PS1(P117L ) reduced
BrdU incorporation the most ( 13 to 14 % ) followed by
PS1(P267S ) ( 7.5 to 9 % ) , with the
PS1(E280A ) mutant inhibiting
BrdU incorporation the least ( 6 to 7 % ) , compared to wild - type
PS1 . -
pubmed
METHODS AND RESULTS : At a concentration of 1 mmol , only
mercury showed significant inhibitory effects on the in vitro enzyme activities ; in all other cases , the
enzymes remained active . -
pubmed
Lipoxin - mediated inhibition of
IL-12 production by DCs : a mechanism for regulation of microbial immunity . -
pubmed
Phlorizin - specific binding and Western blot analysis indicated a decrease in the amount of
SGLT1 in the ileum when compared to the duodenum and jejunum . -
pubmed
We can conclude that the decrease in the apical
D - glucose transport found in the ileum is primarily due to a reduction in the amount of
SGLT1 present in the brush - border membrane rather than the differences in the lipid composition and fluidity . -
pubmed
Suppression of
monocyte chemoattractant protein 1 , but not
IL-8 , by
alprazolam : effect of
alprazolam on
c - Rel / p65 and
c - Rel / p50 binding to the
monocyte chemoattractant protein 1 promoter region . -
pubmed
The goal of this study was to elucidate whether
alprazolam modulates
IL-1alpha - initiated responses . -
pubmed
We found that
alprazolam inhibited
IL-1alpha - elicited
MCP-1 production within a range of 0.1 - 3 micro M. In contrast , it did not inhibit
IL-1alpha - induced
IL-8 production . -
pubmed
Although
NF - kappaB is involved in regulating the
IL-1alpha - induced expression of
MCP-1 and
IL-8 , the degradation of
IkappaB - alpha stimulated by
IL-1alpha was not inhibited by
alprazolam . -
pubmed
Alprazolam prevented
NF - kappaB from binding to the MCP-1 promoter region ( the
A2 and
A1 oligonucleotide probes ) , but binding of
NF - kappaB to
IL-8 /
NF - kappaB was not inhibited . -
pubmed
Moreover ,
alprazolam inhibited
c - Rel / p50 binding to the
A2 oligonucleotide probe , but not
p50 / p65 from binding to the IL-8 / NF - kappaB site . -
pubmed
While
AP-1 is involved in regulating the
IL-1alpha - induced expression of
IL-8 , but not
MCP-1 ,
alprazolam potentiated the binding of
c - Jun /
c - Fos to the
AP-1 oligonucleotide probe . -
pubmed
These results show that the inhibition of
IL-1alpha - mediated
MCP-1 production by
alprazolam is mainly due to inhibition of
c - Rel / p65 and
c - Rel / p50 binding to the MCP-1 promoter region , since
alprazolam did not affect the
IL-1alpha - mediated activation of
NF - kappaB (
p50 / p65 ) or
AP-1 (
c - Jun /
c - Fos ) binding to the IL-8 promoter region . -
pubmed
In conclusion , a new action of
alprazolam was elucidated , as shown in the inhibition of
c - Rel /
p65- and
c - Rel / p50 - regulated transcription . -
pubmed
In addition cell
death was prevented by antioxidants such as
GSH ,
N - acetylcysteine or
catalase . -
pubmed
When the cells were exposed to
MG132 the level of
Bcl - XL diminished , while a new band , corresponding to the expression of the proapoptotic protein
Bcl - XS was detected . -
pubmed
MG132 also caused the release of
cytochrome c from mitochondria and the activation of
caspase-3 with the consequent degradation of
poly - ADP ribose polymerase (
PARP ) . -
pubmed
The observation that the broad spectrum
caspase inhibitor
z - VAD markedly reduced the apoptotic effect of the drug clearly demonstrated that
caspases play an important role in
MG132 - induced apoptosis . -
pubmed
MG132 exerted a modest effect on the viability of Chang liver cells which primarily depended on the
G2 / M arrest of cell cycle while only a small percentage of apoptotic cells was found . -
pubmed
In response to
ethylene , 9.5
cellulase was also induced in the vascular traces of the stem and the pulvinus without developing a separation layer . -
pubmed
Low serum
calcium was associated with elevated
PTH- ( r = -0.75 , p = 0.001 ) , serum cross laps- ( r = -0.61 , p = 0.01 ) ,
osteocalcin levels ( r = -0.49 , p = 0.05 ) , was an independent predictor of femoral neck bone mass ( r = 0.57 , p = 0.02 ) and accounted for 36 % of this variance . -
pubmed
Serum
OPG was , however , significantly correlated to serum cross laps ( r = 0.71 , p = 0.003 ) ,
osteocalcin ( r = 0.63 , p = 0.01 ) ,
serum parathyroid hormone ( r = 0.61 , p = 0.01 ) and serum
creatinine levels ( r = 0.53 , p = 0.04 ) and showed only a weak and non - significant correlation to femoral neck Z - scores ( r = -0.38 , p = 0.16 ) . -
pubmed
Multiple regression analysis revealed that serum
OPG was correlated independently of
PTH , serum
calcium and
creatinine to serum cross - laps concentrations ( r = 0.63 , p = 0.04 ) . -
pubmed
Liver fat content ( spectroscopy ) ,
serum insulin ,
C - peptide and
triglyceride concentrations were significantly higher , and
HDL cholesterol concentration lower in the HAART+LD+ than the HAART+LD- group . -
pubmed
Dispersed anterior pituitary cells were incubated with or without
fluorescein - conjugated
CRH (
FL - CRH ) . -
pubmed
[
Chloramphenicol -
trypsin association in local treatment of chronic
phlogosis of the sinal cavity and of the ear ] . -
pubmed
Ligands of the
TLR include exogenous microbial components such as LPS (
TLR4 ) , lipoproteins and peptidoglycans (
TLR1 , - 2 , - 6 ) , viral RNA (
TLR3 ) , bacterial and viral unmethylated cytosin -
guanosin dinucleotide ( CpG)-DNA (
TLR9 ) , and endogenous molecules including
heat - shock proteins and extracellular matrix molecules . -
pubmed
Electron microscopy of the lens cytoskeleton from 129S2 / SvPas revealed similar morphological changes in the cytoskeleton as compared to the
CP49 knockout , with beaded and intermediate filaments being apparently replaced by poorly defined filament - like material . -
pubmed
Proteins secreted from the
Ysa TTSS (
Ysps ) have only been detected in vitro when cells are cultured at 26 degrees C in a high -
NaCl medium . -
pubmed
Furthermore , in the absence of
NaCl , the activity of
ysaE -
lacZ was reduced 25 - fold in the wild - type and
DeltaysrS strains , indicating that
YsrS is probably required for the salt - dependent expression of the
ysa locus . -
pubmed
Pre- and posttreatment with elaterium and
cucurbitacin B reduced
CCl4 -
hepatotoxicity , as shown reduction in the anormally increased
sGPT levels . -
pubmed
Using tryptic phosphopeptide mapping , we have identified six additional
CHK1 and
CHK2 sites residing in the final 100
amino acids of
p53 . -
pubmed
Phosphorylation of at least three of these sites ,
Ser366 ,
Ser378 , and
Thr387 , was induced by DNA damage , and the induction at
Ser366 and
Thr387 was abrogated by small interfering RNA targeting
chk1 and
chk2 . -
pubmed
Exogenous
creatine was taken up by keratinocytes and increased
CK activity , mitochondrial function and protected against free
oxygen radical stress . -
pubmed
Upon
butyrate treatment , the
cyclin E - over - expressing cells exhibited a significantly increased
albumin - secreting and
ammonia - detoxifying capacity when compared to the control cells . -
pubmed
OBJECTIVES : : The aims of this study were to improve the resolution of
albumin variants (
bisalbumins , especially
albumin Naskapi ) from
albumin in commercial serum protein electrophoresis systems ; to compare the separation of
bisalbumin from
albumin on these improved methods against the recognized gold standard of capillary electrophoresis ; and to investigate the presence of
albumin variants in urine . -
pubmed
In response to changes in environmental factors such as
oxygen tension , blood flow , circulating
cytokines , and growth factors , the endothelium synthesizes and/or extracts many vasoactive mediators such as
endothelin-1 (
ET-1 ) ,
norepinephrine ,
angiotensin 1 ,
thromboxane ,
prostacyclin (
PGI ( 2 ) ) , and the endothelial - derived relaxing factor
nitric oxide (
NO ) . -
pubmed
Among the PDF groups , cellular
adhesion molecule expressions on ECs and
CD11b expression on PMNs were lower with 600 and 1000 micromol / L than with 300 micromol / L
GLN . -
pubmed
IL-8 secretions from ECs and PMNs were higher with 300 and 600 micromol / L than with 1000 micromol / L
GLN , and this was consistent with the expression of the
IL-8 receptor on PMNs . -
pubmed
A low
GLN concentration comparable to catabolic conditions resulted in higher
adhesion molecule expression and greater transendothelial migration of neutrophils . -
pubmed
Body mass index (
BMI ) and lipid profile including total
cholesterol ,
low density lipoprotein cholesterol ,
high density lipoprotein cholesterol and
triglyceride correlated with blood pressure . -
pubmed
RESULTS : The biological activity of
PC - PLD was significantly decreased by
TNF - alpha and
ceramide ( P< ; 0.05 ) . -
pubmed
On the other hand , the GMC apoptosis was induced by
TNF - alpha and
ceramide ( P & lt;0 . -
pubmed
Methods We compared total nucleated cell (
TNC ) and
CD34(+ ) cell counts before and after processing with three different CBU volume reduction methods used consecutively in our bank : a manual method based on
hydroxyethyl starch sedimentation ( HES ) ( n=447 ) , a top - and - bottom ( TB ) semi - automated method ( n=181 ) using Optipress
II , and the Sepax automated method ( n=213 ) . -
pubmed
Gliding movements of the leading
poles of
cephalexin - treated filamentous cells were observed but not equivalent movements of the lagging
poles . -
pubmed
Indole-2 - amide based biochemical antagonist of
Dishevelled PDZ domain interaction down - regulates
Dishevelled - driven
Tcf transcriptional activity . -
pubmed
We designed and synthesized a series of
indole-2 - amide - based compounds that antagonize interaction between the
Dishevelled (
Dvl ) PDZ domain and a peptide derived from the natural PDZ ligand
Frizzled-7 (
Fz7 ) . -
pubmed
46 : 1303 - 1311 ) characterized the properties of secondary structure and in vitro functions of different
cysteine mutants of
apolipoprotein A - I. -
pubmed
Chondrocytes stimulated with
tumor necrosis factor - alpha (
TNF - alpha ) plus
Ro 31 - 8220 ( a specific inhibitor of
mitogen - activated kinase phosphatase-1 -
MKP-1 ) were employed as an in vitro model of apoptosis . -
pubmed
Protein phosphatase 2A (
PP2A ) activity was estimated by measuring the absorbance of a
molybdate :
malachite green :
phosphate reaction complex . -
pubmed
The integral membrane subunit
PsrC was characterized using the natural substrate
menaquinone-7 and inhibitors , providing a comprehensive representation of a
quinone binding site and revealing the presence of a water - filled cavity connecting the
quinone binding site on the periplasmic side to the cytoplasm . -
pubmed
SIGNIFICANCE : The degree of nanoleakage had no influence on microtensile bond strength for the Syntac classic / Tetric Ceram Cavifil or for the Prime & amp ; Bond
NT / Spectrum
TPH group . -
pubmed
BACKGROUND : The aim of this study was first to compare the widely used flow mediated dilation (
FMD ) method with the iontophoretically induced
acetylcholine vasodilation ( IAV ) procedure . -
pubmed
Part
II : Isolation of the linear
9 - formylcarboxylic acid and its sequential macrocyclization with an
amino alcohol or an
azidoamine . -
pubmed
Although optimization of 15 - membered
azalactams including
demycarosyl analogues did not provide remarkably promising molecules ,
SAR studies of
16 - membered azalides disclosed that substitution at the 15 position was very important for identification of a clinical candidate . -
pubmed
The
5 ' triphosphate - ended virus leader transcript is recognised by
RIG - I , a cellular
helicase , and induces the
interferon response . -
pubmed
One - dimensional
( 1 ) H magnetic resonance spectroscopy (
MRS ) experiments were performed on the supernatant of aqueous dispersions of the stool samples . -
pubmed
Using SR1 - specific
16S rRNA gene primers , we identified multiple novel SR1 lineages in four different anaerobic environments : sediments from Zodletone Spring , a
sulfide- and
sulfur - rich
spring in southwestern Oklahoma ; inner layers of microbial mats obtained from Sperm Pool , a high - temperature , low - pH pool ( 55 degrees C , pH 2.5 ) in Yellowstone National Park ; fresh bovine ruminal contents ; and anaerobic freshwater pond sediments ( Duck Pond ) in Norman , Oklahoma . -
pubmed
Corneal verticillata after dual anti -
epidermal growth factor receptor and anti -
vascular endothelial growth factor receptor 2 therapy (
vandetanib ) for
anaplastic astrocytoma . -
pubmed
Protein denaturation is preceded by an initial loss of water from the first solvation shell and consequent in - flow of
urea toward the
protein . -
pubmed
Urea reaches the first solvation shell of the
protein mainly due to electrostatic interaction with a considerable contribution coming from the dispersion interaction . -
pubmed
We have also seen that water follows
urea and reaches the protein interior at later stages of denaturation , while
urea preferentially and efficiently solvates different parts of the
protein . -
pubmed
Why
urea is preferred over water for binding to the
protein backbone and how
urea orients itself toward the
protein backbone have been identified comprehensively . -
pubmed
Tumors with 13q34 amplification were associated with high grade ,
estrogen receptor negativity , and expression of
EGFR ,
CCNE ,
CK5 , and
P - Cadherin , among other basal cell markers . -
pubmed
A potential regulatory single
nucleotide polymorphism in the promoter of the
Klotho gene may be associated with
essential hypertension in the Chinese Han population . -
pubmed
AIM : To compare the efficacy of different solutions ( 1 %
sodium hypochlorite , 10 %
citric acid and 20 %
EDTA ) in the removal of
calcium hydroxide from root canals . -
pubmed
Following the selection of reference genes , the novel target genes
E2F7 and
IL-11RA were identified as potential toxicity biomarkers for
acetaminophen treatment . -
pubmed
grisea
infection and following exogenous
jasmonate ( JA ) or
salicylic acid (
SA ) exposure in the old leaves , but not in the new leaves of the wild - type . -
pubmed
These are :
PenB_
TUA1 coding for an
alpha - tubulin family protein ,
PenB_
Raba1/11 coding for a
Rab family protein ,
PenB_HMG - box coding for an
HMG - box family protein and
PenB_
MT coding for an
unknown transcript that contains an ORF of 295
amino acid residues . -
pubmed
Small - angle X - ray diffraction ( SXRD ) , infrared spectroscopy ( IR ) ,
nitrogen adsorption / desorption , transmission electron microscopy (
TEM ) , thermogravimetric analysis (
TGA ) , and inductively coupled plasma atomic emission ( ICP - AES ) measurements allow us to propose that the
organotin species are located within the pore channels of the mesoporous host . -
pubmed
Results of redundancy analysis ( RDA ) showed that
silicate (
SiO ( 4 ) ) and soluble reactive
phosphorus ( SRP ) were the most important environmental factors influencing the phytoplankton distribution during
spring and summer , respectively . -
pubmed
Comparison of peritonsillar infiltration effects of
ketamine and
tramadol on
post tonsillectomy pain : a double - blinded randomized placebo - controlled clinical trial . -
pubmed
AIM : To assess the effect of peritonsillar infiltration of
ketamine and
tramadol on
post tonsillectomy
pain and compare the side effects . -
pubmed
CONCLUSION : Preoperative peritonsillar infiltration of
tramadol can decrease
post - tonsillectomy
pain , analgesic consumption , and the time to recovery without significant side effects . -
pubmed
Levels of
PGC-1alpha were correlated negatively ( P<0.05 ) with total
cholesterol levels , aortic
superoxide anion production and
tumour necrosis factor - alpha expression , and positively ( P<0.05 ) with maximal relaxation in response to
acetylcholine . -
pubmed
OBJECTIVES :
Tacrine was the first
acetylcholinesterase inhibitor approved for the treatment of
Alzheimer disease . -
pubmed
Parameters such as
ferric reducing antioxidant power ( FRAP ) ,
thiobarbituric acid reactive substances ( TBARS ) , carbonylated proteins ,
caspase 3 activity ,
superoxide dismutase activity and
glutathione reductase activity were assessed in the frontal , temporal and occipital lobe , cerebellum , liver , spleen , heart , and kidney . -
pubmed
Moreover , levels of
glucose , total and
HDL cholesterol forms ,
triglycerides , blood
urea nitrogen ,
creatinine , total
bilirubin ,
total protein ,
albumin and activities of
aspartate aminotransferase ,
alanine aminotransferase ,
alkaline phosphatase and
lactate dehydrogenase were assessed in plasma samples . -
pubmed
Subsequently , we demonstrate that
LPA is a potent mast cell activator which , unlike other triggers , favors release of
tryptase . -
pubmed
In this study , we report that
Adenine Phosphoribosyl Transferase 1 (
APT1 ) is the causal gene of the high - dose
cytokinin - resistant mutants . -
pubmed
After accounting for age and sex ,
DHEAS was significantly inversely associated with both
anxiety sensitivity ( F(3,92 ) = 6.97 , partial
eta2 = 0.07 , P = 0.01 ) and negative affect ( F(3,87 ) = 10.52 , partial
eta2 = 0.11 , P = 0.002 ) across groups . -
pubmed
The persistent significance of the
androgen receptor (
AR ) axis in
CRPC was recently validated by the clinical efficacy of
androgen synthesis inhibitors (
abiraterone ) and novel , second - generation
AR antagonists (
enzalutamide ) . -
pubmed
The appreciation that conventional therapeutic approaches achieve a suboptimal ablation of intratumoral
androgens and
AR axis signaling output opens transformative therapeutic opportunities . -
pubmed
The levels of
AST ,
CK ,
ALT ,
SOD ,
GSH - pX ,
MDA , miR-146a , and ECG were measured to explore the effects of
SMI in cardiologic function and immune activity . -
pubmed
RESULTS : Results show that the levels of
AST ,
CK , and
MDA decreased upon the administration of
SMI . -
pubmed
The
APS reveals unique optical and electronic properties which can efficiently modify the work function ( PhiW ) of
indium tin oxide (
ITO ) electrode to enhance the electron blocking capability . -
pubmed
The inverted device based on
APS reached a power conversion efficiency ( PCE ) of 3.56 % and exhibited much better stability under ambient conditions relative to that of the corresponding
PEDOT :
PSS based device . -
pubmed
Gene network modeling and characterization of T - DNA mutants indicated that
acyl - activating enzyme encoded by
At4g19010 contributes to the biosynthesis of
ubiquinone specifically from
phenylalanine . -
pubmed
CoA ligase assays verified that
At4g19010 prefers
para - coumarate ,
ferulate , and
caffeate as substrates . -
pubmed
Feeding experiments demonstrated that the
at4g19010 knockout can not use
para - coumarate for
ubiquinone biosynthesis and that the supply of
4 - hydroxybenzoate , the side - chain shortened version of
para - coumarate , can bypass this blockage . -
pubmed
Furthermore , a
trans - cinnamate 4 - hydroxylase mutant , which is impaired in the conversion of
trans - cinnamate into
para - coumarate , displayed similar defects in
ubiquinone biosynthesis to that of the
at4g19010 knockout . -
pubmed
Green fluorescent protein fusion experiments demonstrated that
At4g19010 occurs in peroxisomes , resulting in an elaborate biosynthetic architecture where
phenylpropanoid intermediates have to be transported from the cytosol to peroxisomes and then to mitochondria where
ubiquinone is assembled . -
pubmed
Collectively , these results demonstrate that
At4g19010 activates the
propyl side chain of
para - coumarate for its subsequent beta - oxidative shortening . -
pubmed
Expression of
serine peptidase
inhibitor ,
Kazal type 3 (
Spink3 ) was detected in EPL cell - derived endoderm , and in a band of endoderm immediately distal to the embryonic - extra - embryonic boundary in pregastrula and gastrulating embryos . -
pubmed
The gammadeltaT cells that were positive for
CCR4 were killed by
mogamulizumab via ADCC . -
pubmed
This suggests that the mechanism by which
melatonin alleviates the damage to the spinal cord by
edema might be related to the expression of
aquaporin-4 and
glial fibrillary acidic protein . -
pubmed
Poly - dipeptides encoded by the
C9ORF72 repeats block global protein translation . -
pubmed
Among these ,
poly - proline - arginine ( poly - PR ) and poly -
glycine -
arginine ( poly -
GR ) peptides are known to be
neurotoxic . -
pubmed
The results displayed that the
AMPKalpha1 overexpression suppressed lipid accumulation in the cytoplasm , decreased
triglyceride levels , maintained the survival of
steatotic hepatocyte model with decreased cell apoptosis and increased survival rate . -
pubmed
Finally ,
Anisomycin , a frequently - used activator of
p38MAPK , reversed the inhibitory effect of pc -
AMPKalpha1 on the expression of p -
p38MAPK , suggesting that
AMPKalpha1 overexpression alleviates inflammatory response through the inactivation of
p38MAPK . -
pubmed
Serum and Plasma Levels of
Vascular Endothelial Growth Factors in Relation to Quality of
Glucose Control , Biomarkers of
Inflammation , and
Diabetic Nephropathy . -
pubmed
Levels of
vascular endothelial growth factors (
VEGF ) are regulated in a complex network of adipokines ,
glucose control , and low grade
inflammation together with activated platelets , leucocytes , and
endothelial dysfunction . -
pubmed
T2DM was associated with significantly higher levels of
hsCRP ,
ALAT , and
albumin /
creatinine ratio . -
pubmed
Estrogen ,
relaxin , and
progesterone can alter the balance between tissue degradation and repair by modulating MMPs , leading to gender disparities in many
MMP - related disease states . -
pubmed
In this article , we review the literature that examines the effects of
estrogen ,
relaxin , and
progesterone on
MMP production and activity , connecting the nanoscale , microscale , and macroscale details to relevant disease states . -
pubmed
Once successful transfection was achieved , the effects on the mRNA level of
human telomerase reverse transcriptase (
hTERT ) ,
telomerase activity , cell proliferation and apoptosis were examined by semi - quantitative RT - PCR , stretch PCR ,
MTT assay and flow cytometry , respectively . -
pubmed
Phytochemical profiling of the most active fraction by nontargeted RP - HPLC -
MS and
MS /
MS technique showed that
rosmarinic acid and
methylrosmarinate constituted nearly 51 % of the total metabolites apart from twelve other major chemotypes . -
pubmed
MATE2 Expression Is Associated with
Cancer Cell Response to
Metformin . -
pubmed
Intracellular uptake and retention of
metformin is affected by the expression of
organic cation transporters ( OCT ) 1 - 3 and by multidrug and toxic compound extrusion ( MATE ) 1 - 2 . -
pubmed
PURPOSE : Here we sought to determine if there is an association between the effects of
metformin on inhibiting
oxygen consumption , proliferation and expression of
OCTs and
MATEs in a panel of 19
cancer cell lines . -
pubmed
There were statistically significant associations between resistance to inhibition of proliferation and
MATE2 expression , as well as between sensitivity to inhibition of
oxygen consumption and
OCT3 expression . -
pubmed
High
MATE2 expression may result in resistance to the anti - proliferative effect of
metformin and should be considered as a negative predictive biomarker in clinical trials . -
pubmed
EglN2 contributes to
ERalpha - positive breast
tumorigenesis by acting as an
estrogen - inducible gene . -
pubmed
Furthermore , we identified some potential
serine or
threonine residues on the C - terminal of
EglN2 that may mediate its binding and potential regulation by
FBW7 . -
pubmed
In regards to
T2DM - related
metabolic complications , the AG and GG genotypes of rs731236 were significantly associated with higher total
cholesterol ( p=0.011 ) and
LDL -
cholesterol ( p=0.009 ) levels in the patients with
T2DM . -
pubmed
In contrast , the
CT genotype of rs1544410 was significantly associated with lower
BMI ( p=0.031 ) and the TT genotype was associated with lower
LDL -
cholesterol level ( p=0.007 ) . -
pubmed
The rs731236 variant was shown to be associated with high
cholesterol and
LDL -
cholesterol levels in
T2DM patients , while rs1544410 was associated with lower
BMI and lower
LDL cholesterol levels . -
pubmed
These preliminary results of biexponential
T2 analysis could potentially be used to increase the specificity for detection of early
osteoarthritis by measuring different
water compartments and their fractions . -
pubmed
CONCLUSIONS : In this preclinical model , the new biodegradable polymer - based
DES studied showed less
restenosis than BMS and no significant differences in safety or efficacy vs commercially available
DES . -
pubmed
Sensitive and Label - Free Fluorescent Detection of
Transcription Factors Based on DNA -
Ag Nanoclusters Molecular Beacons and
Exonuclease III - Assisted Signal Amplification . -
pubmed
Herein , we first applied DNA -
Ag nanoclusters molecular beacons ( AgMBs ) in TFs analysis and designed an assay based on the switchable fluorescence of
AgMBs . -
pubmed
Then , the reporter triggers downstream Exo III - assisted signal amplification by continuously consuming the
guanine - rich enhancer sequences in
AgMBs , resulting in significant fluorescent decrease eventually . -
pubmed
DNA was extracted from peripheral white blood cells and methylation levels of nine
CpG sites in
NET gene promoter region were investigated by pyrosequencing . -
pubmed
RESULTS : For each
CpG site and the average value of nine
CpG sites , there were no significant differences in DNA methylation of the
NET gene promoter between healthy controls and patients with
depression or
hypertension . -
pubmed
CONCLUSION : Our study indicates that DNA methylation levels of nine
CpG sites in
NET gene promoter region are not associated with
depression and
hypertension . -
pubmed
Deficient
Insulin - mediated Upregulation of the
Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased
Adenosine in
Diabetic Glomerulopathy . -
pubmed
Therefore , we tested a link between
insulin deficiency and dysregulated activity of the
equilibrative nucleoside transporters (
ENTs ) responsible for controlling extracellular levels of
adenosine . -
pubmed
In ex vivo glomeruli , high
D - glucose decreased
nucleoside uptake mediated by
ENT1 and
ENT2 transporters , resulting in augmented extracellular levels of
adenosine . -
pubmed
Particularly ,
insulin through
insulin receptor /
PI3 K pathway markedly upregulated
ENT2 uptake activity to restores the extracellular basal level of
adenosine . -
pubmed
In conclusion , we evidenced
ENT2 as a target of
insulin signaling and sensitive to dysregulation in
diabetes , leading to chronically increased extracellular
adenosine levels and thereby setting conditions conducive to
kidney injury . -
pubmed
Identification of Novel Conjugative Plasmids with Multiple Copies of
fosB that Confer High - Level
Fosfomycin Resistance to
Vancomycin - Resistant Enterococci . -
pubmed
The total copy number of the
fosB gene as revealed by qRT - PCR did not correlate with
fosfomycin MICs or growth rates at sub - MICs of
fosfomycin in different transconjugants . -
pubmed
From susceptibility tests , the
fosB gene , regardless of the copy number , conferred high
fosfomycin MICs that ranged from 16384 to 65536 mug / ml . -
pubmed
This first complete nucleotide sequence of a plasmid carrying two copies of
fosB in VRE suggests that the
fosB gene can transfer to multiple loci of plasmids by the ISL3 family transposase
TnpA , possibly in the form of circular intermediates , leading to the dissemination of high
fosfomycin resistance in VRE . -
pubmed
A combined computational / experimental study on
HSA binding of two water - soluble
Schiff base ligands derived from
pyridine derivative and
ethylendiamine . -
pubmed
The efficacy of
erlotinib treatment for advanced
non - small cell lung cancer (
NSCLC ) is due to its action as an
epidermal growth factor receptor tyrosine kinase inhibitor (
EGFR - TKI ) . -
pubmed
The single - nucleotide polymorphisms ( SNPs ) rs1042640 in
UGT1A10 , rs1060463 , and rs1064796 in
CYP4F11 , and rs2074900 in
CYP4F2 were significantly associated with therapeutic responses to
erlotinib . -
pubmed
Moreover , analysis of a validation cohort confirmed the significant association between rs10045685 in
UGT3A1 and
erlotinib adverse drug response(unadjusted p = 0.015 ) . -
pubmed
The aim of the present study was to formulate a nanosuspension (
FA -
NS ) of
fusidic acid (
FA ) to enhance its aqueous solubility and antibacterial activity . -
pubmed
The molecular dynamics simulation of
FA and
Poloxamer-188 showed an interaction and binding energy of -74.42 kJ / mol and -49.764 + - 1.298 kJ / mol , respectively , with van der Waals interactions playing a major role in the spontaneous binding . -
pubmed
The
MTT assays showed a cell viability of 75 - 100 % confirming the nontoxic nature of
FA -
NS . -
pubmed
Bioavailability was determined by co - administering feed with either pure
PZQ ,
HCO -
SLN or
HCO -
SLN coated with chitosan via intubation and quantifying the pharmacokinetics response . -
pubmed
Furthermore , palatability of the feeds medicated with
PZQ was not improved when the
PZQ was incorporated into HCO -
SLN , possibly due to the low loading rate of
PZQ within the HCO -
SLN and the subsequent thick coating of nanoparticles that was required on the surface of the feed pellets . -
pubmed
Lenalidomide is approved for maintenance and guidelines recommend its use
post ASCT . -
pubmed
In addition to their classical mode of action in the brain , circulating factors may modulate the release of reactive
oxygen /
nitrogen species ( ROS /
RNS ) from endothelial cells that compose the BBB without entering the brain . -
pubmed
Control and early life stressed heterozygous
serotonin transporter knockout (
SERT ) dams were treated with the SSRI
fluoxetine or vehicle throughout gestation and lactation . -
pubmed
Fluoxetine treatment of the dams resulted in a reduced total USV duration in pups at postnatal day 6 , especially in
SERT+/+ males . -
pubmed
Interestingly , early life stress in dams increased adult social exploration in vehicle exposed
SERT+/+ female offspring and total social behavior in
fluoxetine exposed adult
SERT+/- male offspring . -
pubmed
The enzymes
L - asparaginase ,
L - glutaminase , and
L - arginase were chosen according to
amino acids starvation in
cancer cells and screened in halophilic and halotolerant bacteria , given probably less immunological reactions of halophilic or halotolerant enzymes in patients . -
pubmed
Identification of personalized antihypertensive treatments is a key for better BP management Objective : This review aimed to elucidate aspects of rational drug design and other methods to develop better
hypertension management Results : Among
hypertension - related signaling mechanisms , the
renin -
angiotensin -
aldosterone system is the leading genetic target for
hypertension treatment . -
pubmed
The excessive accumulation of
triglyceride (
TG ) and the deposition of fat were the two most critical causes . -
pubmed
Here , we developed
Fe3O4@OA@
Poloxamer nanoparticles ( NPs ) with amphiphilic structures , which exhibited an excellent role in eliminating excess
TG . -
pubmed
Hydrophobic
TG was adsorbed efficiently by
Fe3O4@OA@
Poloxamer NPs through the " liposuction effect " and the formation of NPs@TG complex was then conducted . -
pubmed
Meanwhile , the " nano - enzyme effect " of
Fe3O4 NPs recovered the lipid - regulated proteins including
PPARalpha , further triggering biodegradation pathways of
TG , although the lipid - regulated proteins were obviously inhibited in the high - fat hepatocytes models . -
pubmed
luteum , possessing
epigallocatechin , were active inhibitors of
cholinesterase enzymes ,
alpha - glucosidase , and
tyrosinase . -
pubmed
The data presented here include sea surface temperature (
SST ) , sea surface salinity ( SSS ) , Secchi disk depth ( SDD ) ,
Chlorophyll - a (
Chl - a ) , suspended particulate matter ( SPM ) , mineral suspended solid ( MSS ) and chromophoric dissolved organic matter ( CDOM ) . -
pubmed
To overcome barriers associated with gene delivery , a series of peptides consisting of multifunctional fragments , including a cationic amphiphilic alpha - helical antimicrobial peptide (
AMP ) , a cell penetrating peptide (
CPP ) ,
TAT , a
stearyl moiety , and
cysteine residues , were designed and synthesized for evaluation as non - viral gene vectors . -
pubmed
Stearyl moieties provide an intramolecular hydrophobic environment to promote AMPs to form an alpha - helical conformation in
PBS , and this is beneficial for DNA binding , cellular uptake , and endosomal escape . -
pubmed
64Cu - labeled new
pan - somatostatin receptors (
pan - SSTRs ) probe
PA1 was synthesized , characterized , and evaluated by in vitro and in vivo experiments . -
pubmed
The radiosynthesis , quality control , and preliminary biological evaluation of
[ 64Cu ] NOTA - PA1 have broaden the application of
radiolabeled octreotide for SSTRs imaging , which could act as a potential multisubtypes targeted radiotracer for imaging
SSTRs - positive
tumors . -
pubmed
The biosynthetic pathways for
flavonoids and
terpenoids are well represented in roots , including the complete set of proteins for the MEV and
MEP /
DOXP pathways , and proteins involved in the branches which lead to the synthesis
tricyclic diterpenoids and
gibberellins . -
pubmed
Also ,
casbene synthase which catalyzes the first commited step in the biosynthesis of
tigliane - type
diterpenes was identified in roots of both genotypes , but not in leaves and endosperm . -
pubmed
Cerebral Ischemia -
Reperfusion Injury :
Lysophosphatidic Acid Mediates
Inflammation by Decreasing the Expression of
Liver X Receptor . -
pubmed
The levels of
LPA , an extracellular signaling molecule , increased after
ischemia and caused
neurological injury effect , decreased the expression level of
LXR , and increased the expression level of
inflammatory factors (
IL-1beta ,
IL-6 , and
TNF - alpha ) via the
NFkappaB signaling pathway . -
pubmed
We present a direct or indirect connection between
LPA and
LXR in the pathophysiological process . -
pubmed
In conclusion , we speculate that the inhibition of
LPA generation and administration of
LXR agonist may be explored as potential
cerebral infarction treatment strategies . -
pubmed
Analysis using the Kyoto Encyclopedia of Genes and Genomes database revealed differential genes associated with ribosome function ,
pyruvate metabolism , biosynthesis of
amino acids ,
fatty acid biosynthesis ,
fatty acid metabolism ,
ATP - binding cassette ( ABC ) transporter , glycolysis , and glycerophospholipid metabolism . -
pubmed
RESULTS : The LN , active LN , and stable LN groups had significantly higher levels of anti - dsDNA antibody ,
C - reactive protein , serum
creatinine ( Scr ) , and blood
urea nitrogen (
BUN ) than the control group ( P<0.05 ) . -
pubmed
A potential to extend this versatility further was expected and examined in the present study , based on the recently discovered hysteretic behavior of a widely used chiral selector (
CS ) ,
amylose tris ( 3,5 - dimethylphenylcarbamate ) . -
pubmed
Resveratrol prevents
TNF - alpha - induced
VCAM-1 and
ICAM-1 upregulation in endothelial progenitor cells via reduction of
NF - kappaB activation . -
pubmed
OBJECTIVE : To assess the effects of
resveratrol (
RSV ) on expression of
adhesion molecules in endothelial progenitor cells ( EPCs ) following
tumor necrosis factor - alpha (
TNF - alpha ) stimulation . -
pubmed
A total of 10 - 20 % of plasma membrane proteins are anchored by
glycosylphosphatidylinositol (
GPI ) . -
pubmed
The median values of
HBsAg reduction from baseline were -0.44 ( n=48 ) , -0.41 ( n=40 ) , and -0.68 ( n=11 ) log IU / mL at the end of treatment and at 48 and 144 weeks
post - treatment , respectively . -
pubmed
The rates of
HBsAg response were 24.0 % and 22.5 % at the end of treatment and at 48 weeks
post - treatment , respectively . -
pubmed
A multivariate analysis identified
HBsAg < 3.00 log IU / mL as an independent baseline factor contributing to the
HBsAg response at the end of treatment and 48 weeks
post - treatment ( p=1.07x10 - 2 and 4.42x10 - 2 , respectively ) . -
pubmed
There were significant differences in the reduction of the
HBsAg levels at 12 weeks of treatment and in the incidence of serum
ALT increase during treatment between patients with and without an
HBsAg response . -
pubmed
Apoferritins prepared chemically by removal of
iron from the
holoferritins had migration properties indistinguishable from the parent
holoferritins . -
pubmed
There is an increase in the serum
thyroxine ( T4)and
triiodothyronine ( T3 ) concentration largely due to an increase in
thyroid hormone - binding proteins . -
pubmed
Six male chronic marihuana ( MH ) users exercised on a bicycle ergometer for 15 min at approximately 50 % VO2max under 3 conditions : ( 1 ) not smoking ( control ) , ( 2 ) after smoking MH containing 7.5 mg ( - )
delta-9 - tetrahydrocannabinol , and ( 3 ) after smoking placebo marihuana (
PL ) . -
pubmed
The combined effect of radiotherapy and
neuraminidase - treated
tumor cells on
3 - methylcholanthrene - induced
fibrosarcoma . -
pubmed
Somatostatin delayed the onset of the
motilin response and reduced motor activity in the stomach and the jejunum . -
pubmed
During
somatstatin infusion the
motilin - induced contractile pattern did not migrate to the jejunum . -
pubmed
The effects of
sucrose uptake on the degradation of total cellular protein and of the cytoplasmic enzyme ,
alkaline ribonuclease , were determined for comparative purposes . -
pubmed
Sucrose uptake produced increases of 270 % and 90 % respectively in the rates of synthesis of
arylsulphatase B and
galactosyltransferase , whereas the rate of synthesis of
alkaline ribonuclease was not affected . -
pubmed
In the absence of
sucrose , the apparent half - lives of
arylsulphatase B and
galactosyltransferase were about 30 days and 40h respectively . -
pubmed
After uptake of
sucrose , the half - life of
arylsulphatase B decreased to about 10 days and that of
galactosyltransferase to 10h . -
pubmed
Neither the half - life of
alkaline ribonuclease ( 4 days ) nor the rate of degradation of total cellular protein was affected by the uptake of
sucrose . -
pubmed
Our investigations demonstrated a dose dependent blood
glucose decrease with each
sulfonylurea corresponding with the
insulin levels after intravenous application . -
pubmed
Gliquidone revealed a "
tolbutamide - typed " dynamic both on
insulin secretion and on blood
glucose decrease whereas
gliflumide showed a "
glibenclamide - typed " reaction . -
pubmed
The delayed and more prolonged
insulin decrease , well known for
glibenclamide , was even more distinct following
gliflumide . -
pubmed
Differences of
insulin secretion after
sulfonulureas can be observed only under intravenous conditions . -
pubmed
St 587 ( 0.5 - 30 micrograms ) decreased
TF latency and
PP threshold below base line . -
pubmed
doses of
St 587 & gt ; 3 micrograms , there was an apparent but incomplete return of
TF latency and
PP threshold toward base line . -
pubmed
guanfacine ( alpha 2 agonist ) in the
TF and
PP tests . -
pubmed
Mechanism - based inactivation of
dihydropyrimidine dehydrogenase by
5 - ethynyluracil . -
pubmed
Uracil analogues with appropriate substituents at the 5 - position inactivated
dihydropyrimidine dehydrogenase (
DHPDHase ) . -
pubmed
For example ,
5 - ethynyluracil inactivated
DHPDHase with an efficiency ( kinact / Ki ) that was 500 - fold greater than that for
5 - propynyluracil . -
pubmed
5 - Ethynyluracil inactivated
DHPDHase by initially forming a reversible complex with a Ki of 1.6 + /- 0.2 microM. -
pubmed
Thymine competitively decreased the apparent rate constant for inactivation of
DHPDHase by
5 - ethynyluracil . -
pubmed
The absorbance spectrum of
5 - ethylnyluracil - inactivated
DHPDHase was different from that of reduced
enzyme . -
pubmed
5 - Ethynyluracil inactivated
DHPDHase with a stoichiometry of 0.9 mol of inactivator per mol of active site . -
pubmed
Enzyme inactivated with
[ 2 - 14C ] 5 - ethynyluracil retained all of the
radiolabel after denaturation in 8 M
urea , but lost radiolabel under acidic conditions . -
pubmed
A radiolabeled peptide was isolated from a tryptic digest of the
enzyme inactivated with
[ 2 - 14C ] 5 - ethynyluracil . -
pubmed
The
amino acid residue designated by X was identified as a
cysteine from previous work with
DHPDHase inactivated with
5 - iodouracil . -
pubmed
In contrast to
5 - ethynyluracil ,
5 - cyanouracil was a reversible inactivator of the
enzyme . -
pubmed
5 - Cyanouracil - inactivated
enzyme slowly regained activity ( t1/2 = 1.8 min ) after dilution into the standard assay . -
pubmed
The study of hormonal balance in the presence of pregnancy and labor in females with a history of
infertility treated with
parlodel failed to reveal any negative effect of elevated
prolactin levels on the fetoplacental system . -
pubmed
Sucrose gradient centrifugation of the PIPLC - released
AChE showed a single 8.3 S molecular form , similar to that observed for
AChE solubilized by
Triton X-100 . -
pubmed
PIPLC removed large amounts of
AChE from the surface of intact schistosomula in culture , with no impairment of the viability of the parasite . -
pubmed
Inhibition experiments with
carbon monoxide and
n - octylamine indicated that this sulphoxidation is catalyzed by
cytochrome ( s ) P-450 , whereas
flavin - containing monooxygenase and/or non - enzymatic reactions ( via
peroxides ) seems not to be involved : no inhibition was observed by
methimazole ,
N , N - dimethylaniline , preheating or
glutathione and
EDTA . -
pubmed
Aristolochic acid is a non - competitive inhibitor of
PLA2 . -
pubmed
The binding of
aristolochic acid to
PLA2 induces an extrinsic CD band at 320 nm . -
pubmed
Aristolochic acid forms a 1:1 complex with
PLA2 , with an association constant K , of 5.4 X 10(3 ) M-1 and a Gibb 's free energy change ( delta G0 ) for the reaction of -5.1 kcal / mole . -
pubmed
Binding of
aristolochic acid causes a change in the secondary structure of the
protein which is characterized by an increase in the apparent content of alpha - helix , without any detectable change in the tertiary structure of
PLA2 . -
pubmed
Whereas
steroids exerted limited , possibly dose - dependent effects on this sequence ,
cyclophosphamide seemed to cause normalization of
OKT4+/
OKT8 + ratio , precipitous drop of
anti - ds - DNA - Ab titers , and dramatic clinical improvement . -
pubmed
p - Phenylenediamine ,
o - dianisidine (
o - DA ) and
3,5,3 ' , 5 '- tetramethylbenzidine were found to be optimal substrates of
horse - radish peroxidase . -
pubmed
The kinetics of oxidation of these substrates by
horseradish peroxidase modified with three molecules of
Strophanthin K was studied as well . -
pubmed
Within the temperature range from 37 to 53 degrees C the inactivation rate constants were determined for
peroxidase and its conjugate with
Strophanthin K. The effect of
sugars and
polyols on thermal stability of the
conjugate peroxidase -
Strophanthin K was investigated . -
pubmed
Measurements of
glucose utilization during constant infusions of
insulin , showed significant changes with changing energy state , falling with overeating and rising with undereating . -
pubmed
Insulin sensitivity was not correlated with plasma
norepinephrine metabolism . -
pubmed
We have compared surface charge and the surface charge density on the polyanions heparin and
potassium polyvinyl sulfate (
KPVS ) , as well as on hydrolyzed heparin and KPVS , with their accelerating effect on the inhibitory action of
antithrombin III on
thrombin . -
pubmed
When the
post -
phenobarbitone half - lives were adjusted to a standard height they were approximately normally distributed . -
pubmed
There was a significant regression of mean offspring value on mid - parent value , indicating that about 65 % of the observed phenotypic variance of
post -
phenobarbitone plasma
phenylbutazone half - lives is due to the additive effects of genes . -
pubmed
We measured
CBF by a modification of the venous occlusion plethysmography technique and found that
CBF increased 7.8 % per Torr alveolar
carbon dioxide pressure change and that it decreased 15 % with 100 %
O2 . -
pubmed
These findings suggest that 1 )
CO2 is an important regulator of
CBF in the perterm infant , 2 )
CBF -
CO2 sensitivity in these infants may be greater than in adult subjects , 3 ) 100 %
O2 reduced
CBF significantly , and 4 ) a decrease in
CBF during administration of 100 %
O2 may be at least partially responsible for the increase in ventilation with
hyperoxia . -
pubmed
In the complicated cases of
myocardial infarction it was established that the extraction of
glucose ,
insulin decrease and in a number of cased production of
lactate by the myocardium supervenes . -
pubmed
The mean maternal serum
glucose was 124.3 mg / dl ( range : 72.7 to 281.5 mg / dl ) , the mean
hemoglobin A1c was 6.03 % ( range : 3.3 to 11.0 % ) and the mean
fructosamine level was 255.97 mumol / L ( range : 205 to 397 mumol / L ) . -
pubmed
Additionally , no significant correlation was noted between RI values and serum
glucose levels ( r = 0.03 ) ,
hemoglobin A1c levels ( r = 0.13 ) or
fructosamine levels ( r = 0.04 ) during the period of 26 to 34 gestational weeks . -
pubmed
Since the intracellular activity of
glutamine synthetase is significantly decreased after the administration of
APAP to hepatocytes in culture , it is likely that the arylation of this enzyme may be involved in the ensuing
hepatotoxicity . -
pubmed
The enzyme was proven to be a
metalloprotease like
protease III and
IDE , as was shown by the inhibitory effects exerted by
EDTA and
o - phenanthroline . -
pubmed
Similar to
protease III and
IDE , ICP prefers the cleavage of small polypeptides (
insulin ,
insulin B - chain ,
glucagon ) to the cleavage of proteins (
casein ,
human serum albumin ,
globin ) and was inactive against synthetic
amino acid derivates (
esters ,
p - nitranilides , and
furoylacroleyl substrates ) of
subtilisin ,
thermolysin ,
trypsin , and
chymotrypsin . -
pubmed
Cleavage sites in the oxidized
insulin B - chain generated by ICP are
Asn3 - Gln4 ,
His10 - Leu11 ,
Ala14 - Leu15 ,
Leu17 - Val18 ,
Gly23 - Phe24 ,
Phe24 - Phe25 , and
Phe25 -
Tyr26 . -
pubmed
Repression of
angiotensin II and potentiation of
bradykinin contribute to the synergistic effects of dual
metalloprotease inhibition in
heart failure . -
pubmed
Blockade of the formation of
angiotensin II (
Ang II ) acting together with potentiation of the natriuretic peptides ,
bradykinin and other vasoactive peptides may mediate the interaction of dual enzyme inhibition . -
pubmed
The
Ang II AT1 receptor antagonist ,
SR 47436 (
BMS-186295 ) , was administered at 30 mumol / kg , i.v . -
pubmed
infusion at 1 mumol / kg / min in combination with
NEP - I (
SQ-28603 at 30 mumol / kg i.v . ) -
pubmed
Bradykinin B2 receptor antagonism by
Hoe 140 at 100 micrograms / kg , i.v . -
pubmed
significantly blunted the decrease in left ventricular end
diastolic pressure but not the decrease in left ventricular
systolic pressure after dual
NEP - I / ACE - I (
SQ-28603 and
enalaprilat each at 30 mumol / kg , i.v . ) -
pubmed
This suggests that
bradykinin potentiation contributes to the preload - reducing , but not the afterload - reducing , acute effects of
NEP - I /
ACE - I. -
pubmed
Following
tyrosine phosphorylation
IRS-1 binds to and activates specific proteins containing SH2 domains . -
pubmed
Mechanism of enzymic
glycoside hydrolysis and of
glycosyl transfer by
glycosidases and
glycosyltransferases . -
pubmed
Fluctuations in
elastase of cervical mucus and clinical efficacy of
cefodizime in
obstetric and gynecologic infections . -
pubmed
Clinical efficacy of
cefodizime (
CDZM ) in obstetric and
gynecologic infections and in their prevention , and fluctuations in cervical mucus
elastase in
gynecologic infections and during pregnancy were studied , and the following results were obtained : 1 . -
pubmed
Cervical mucus
elastase in
uterine and pelvic infections showed high values before administration of
CDZM but tended to decline with the progress of cure ( 15/19 cases , 78.9 % ) . -
pubmed
In the whole population , there was a statistically significant decrease of
PTH , total
calcium , and proteins at nearly every time of blood withdrawal , when compared with the preoperative levels . -
pubmed
The
PTH decreased earlier and total
calcium levels were significantly lower after total thyroidectomy than after hemithyroidectomy ( at 48 , 72 , and 96 hours ) . -
pubmed
Deficiencies of
copper ,
magnesium ,
vitamin B6 ( activated by a
zinc kinase ) inhibit the conversion of
tryptophan to
nicotinic acid . -
pubmed
This minor form (
AChEII ) was distinguished from the more abundant tetrameric form (
AChEI ) by a higher catalytic subunit relative molecular mass ( M(r ) ) of 80,000 ( 80 K ) , and by a lower affinity for
edrophonium - Sepharose . -
pubmed
Thus , it is possible that
AChEII may be a solubilised form of a
dimeric glycosylphosphatidyl inositol - linked
AChE. -
pubmed
Natural killer ( NK ) cells take up chylomicrons ( CM ) , very low density (
VLDL ) , low density ( LDL ) , high density ( HDL ) and
acetyl - modified low density ( AcLDL ) lipoproteins through different receptors ,
VLDL being the lipoprotein with the highest uptake and
HDL the lowest . -
pubmed
Although the uptake of lipoproteins assessed by flow cytometry using
DiI is not very high , the lipoproteins are able to induce an increase in proliferative responses ,
VLDL , AcLDL and
HDL being the most important ones with 12- and 17 - fold increments , respectively . -
pubmed
Incubation of these cells for 48 h with either
13 - cis retinoid acid (
13 - cis RA ) or
all - trans retinoic acid (
ATRA ) resulted in marked inhibition of cell growth , determined by
3H - thymidine incorporation , and in down - regulation of
CD25 expression , determined by flow cytometry . -
pubmed
pylori cultures contained
NADH and
NADPH oxidase activity . -
pubmed
and the
muscarinic antagonist
l - methylscopolamine i.c.v . -
pubmed
Inhibition of 14 - kDa
PLA2 by
2 - acylamino - alkylphospholipids : the influence of
amide acidity . -
pubmed
2 - Acylamino - alkyl phospholipids are potent competitive inhibitors of 14 - kDa
phospholipases A2 ( e.g. -
pubmed
As concluded from X - ray studies the
amide hydrogen of these inhibitors forms a
hydrogen bond to
His-48 in the active site of the
enzyme . -
pubmed
Comparison of isoacidic and isosteric inhibitors reveals that ( i ) the
hydrogen bond of the
amide proton to
His-48 is crucial for strong
PLA2 inhibition , ( ii ) regardless of the headgroup unsubstituted
N - acyl groups result in optimal
amide acidity for
PLA2 inhibition and ( iii ) the exceptionally strong inhibition by
acetamides and the isosteric
fluoroacetamides is due to an additional steric effect . -
pubmed
Thirty - one single
amino acid substitutions at 24 positions of
FLT3L either enhanced or reduced activity in receptor binding and cell proliferation assays . -
pubmed
In the present study , the effect of
beta - naphthoflavone (
BNF ) ,
phenobarbital (
PB ) and
dexamethasone (
DEX ) on the expression of three
cytochrome P450 (
P450 ) isoforms ,
1A1 ,
2B1 and
3A2 , and on glycogen storage was investigated in intrasplenic liver cell explants in comparison to adult liver . -
pubmed
After
BNF administration a
P450 1A1 expression was induced in the hepatocytes of the peripheral regions of the liver lobules ( zone I , according to Rappaport ) , whereas the staining of the hepatocytes around the central veins disappeared . -
pubmed
After
BNF treatment a staining for
P450 1A1 was induced and also the
P450 2B1 expression was slightly more pronounced . -
pubmed
PB treatment caused an increase in the staining for
P450 2B1 and
3A2 and
DEX administration for
P450 3A2 within the transplanted hepatocytes . -
pubmed
Additionally , after
DEX treatment some bile ducts of the explants displayed a slight staining for
P450 1A1 ,
2B1 and
3A2 . -
pubmed
We observed that the amount of
Ca2 + taken up by the vesicles increases with the magnitude of the DeltapH across the membrane , particularly at
Ca2 + concentrations ( approximately 500 microM ) found optimal for the
antiporter activity . -
pubmed
Under normal conditions ( H+ pump - induced DeltapH ) , the electrochemical gradient dependence of
Ca2 + uptake by the vesicles was checked by inhibition of the process with specific inhibitors (
bafilomycin A ( 1 ) ,
ergocryptin ,
folymicin ,
DCCD ) of the
H (+)- pump activity . -
pubmed
These results indicate that synaptic vesicles
Ca2 +/H+ antiport is indirectly linked to
ATP hydrolysis and it is essentially dependent on the chemical component ( DeltapH ) of the electrochemical gradient generated by the
H (+)- pump activity . -
pubmed
METHODS : Percent fat , fat mass , and fat - free mass (
FFM ) were determined from DXA(QDR ) and DXA(DPX - L ) and from a four - component model based on measures of body density from underwater weighing , body water from
deuterium dilution , and BMC from DXA(QDR ) ( 4C(QDR ) ) or DXA(DPX - L ) ( 4C(DPX - L ) ) in young men ( N = 14 ) and women ( N = 10 ) . -
pubmed
The topology of the rooted tree and the species distribution of
lipocalins suggest that the newly arising
lipocalins show a higher rate of
amino acid sequence divergence , a higher rate of gene duplication , and their internal pocket has evolved towards binding smaller hydrophobic ligands with more efficiency . -
pubmed
A
JAK3 - specific inhibitor blocked
OSM - stimulated
STAT1 tyrosine phosphorylation , DNA - binding activity of
STAT1 as well as
collagenase-1 (
MMP-1 ) ,
stromelysin-1 (
MMP-3 ) ,
collagenase-3 (
MMP-13 ) , and
TIMP-3 RNA expression . -
pubmed
In contrast , a
JAK2 - specific inhibitor ,
AG490 , had no impact on these events . -
pubmed
Similarly ,
curcumin (
diferuloylmethane ) , an anti - inflammatory agent , suppressed
OSM - stimulated
STAT1 phosphorylation , DNA - binding activity of
STAT1 , and
c - Jun N - terminal kinase activation without affecting
JAK1 ,
JAK2 ,
JAK3 ,
ERK1 / 2 , and
p38 phosphorylation . -
pubmed
Curcumin also inhibited
OSM - induced
MMP-1 ,
MMP-3 ,
MMP-13 , and
TIMP-3 gene expression . -
pubmed
MOZ itself is a 2004 - residue protein containing a putative
acetyl CoA - binding motif , so it was hypothesized that
MOZ is a
HAT . -
pubmed
Smaller central pools of
cholesterol later in life may contribute to reduced
low - density lipoprotein -
cholesterol ( LDL - C ) concentrations in the plasma and thus reduce the risk of
cardiovascular disease . -
pubmed
Both plasma total- and
LDL - C were higher ( P & lt;.04 ) in BF compared with RF+
cholesterol and RF formula - fed groups at 4 months of age . -
pubmed
The recombinant
sLDLR was identified by
SDS - PAGE , Western blot and Ligand binding blot in supernatant of GS115 / pPIC9K - sLDLr . -
pubmed
The
SDS - PAGE and Western blot analysis showed that the apparent molecular weight of expressed
sLDLR was about 36 kD. -
pubmed
Limited proteolysis under catalytic condition ( in presence of
PEP ,
Mg2 + and
HCO3 ) although yielded a truncated subunit of 90 kDa , did not affect the catalytic function appreciably but desensitized the
enzyme to the effectors like
glucose-6 - phosphate glycine and
malate . -
pubmed
Development of gene therapy using
prostate - specific membrane antigen promoter / enhancer with Cre Recombinase / LoxP system for
prostate cancer cells under
androgen ablation condition . -
pubmed
To enhance the efficacy of suicide gene therapy for
prostate cancer under
androgen deprivation , we designed a promoter system that consists of the
prostate - specific membrane antigen (
PSMA ) promoter / enhancer ( PEPM ) and Cre - loxP DNA recombination system . -
pubmed
These findings indicate that the combination of the
PSMA promoter / enhancer and the Cre - loxP system can enhance the
PSMA promoter activity even under
androgen ablation conditions and can exert its anti -
tumor effect both in vitro and in vivo . -
pubmed
Counteracting effects of
urea and
methylamines in function and structure of
skeletal muscle myosin . -
pubmed
We have investigated the effects of
urea and the
methylamines trimethylamine oxide (
TMA - O ) and
glycine betaine (
betaine ) on activity and structure of
skeletal muscle myosin . -
pubmed
K (+ ) EDTA ATPase activity of
myosin was almost completely inhibited by
urea ( 2 M ) ;
TMA - O stimulated
myosin activity , whereas
betaine had no effect . -
pubmed
When combined with
urea ( 0 - 2 M ) ,
TMA - O or
betaine ( 1 M ) effectively protected the
ATPase activity of
myosin against inhibition . -
pubmed
Intrinsic fluorescence measurements showed that in
urea or
TMA - O ( 0 - 2 M ) , there were no shifts in the center of mass of the fluorescence spectrum of
myosin , despite a decrease in fluorescence intensity . -
pubmed
Both
methylamines ( & gt;or=1 M ) were also able to protect
myosin structure against
urea - induced effects ( 2 - 8 M ) . -
pubmed
Alteration of the expression of
Bcl-2 ,
Bcl - x ,
Bax ,
Fas , and
Fas ligand in the involved skin of
psoriasis vulgaris following topical
anthralin therapy . -
pubmed
In this study , we examined the immunohistochemical expression of
apoptosis - regulated protein in the involved
psoriatic skin following topical
anthralin therapy . -
pubmed
Immunohistochemical examination revealed that psoriatic keratinocytes expressed high levels of
Bcl - x , which was significantly reduced after
anthralin treatment . -
pubmed
Bax was not detected in the epidermal keratinocytes in the
petrolatum - treated skin , while it was present in the upper keratinocytes after
anthralin therapy . -
pubmed
Bcl-2 was detected only in basal layers of psoriatic epidermis following both
petrolatum and
anthralin application . -
pubmed
Psoriatic keratinocytes expressed higher levels of
Fas in the lower epidermis , while only weak expression was detected in
anthralin - treated plaques . -
pubmed
Furthermore ,
anthralin - treated psoriatic epidermis did not express
FasL. -
pubmed
[ Regulation by
ATP of a
phospholipase D in the ductal cells of the submaxillary gland ] . -
pubmed
Among
ATP analogs ,
BZATP and
ATPgammaS were the only ones able to stimulate the
PLD activity . -
pubmed
PLD stimulation by
ATP was inhibited by
calphostin C and
chelerythrine , two
PKC inhibitors . -
pubmed
The
PLD stimulation by
BZ - ATP and
TPA , a
phorbol ester which activates
PKC , were not additive . -
pubmed
Inductions of
PPARgamma ( 2 ) in WAT and
PPAR beta / delta in BAT are blunted by a hypolipemic drug , the
ciprofibrate . -
pubmed
Unexpectedly , treatment with
ciprofibrate deeply affects lipolysis in BAT by increasing
acyl - CoA dehydrogenase activity ( 3.4 fold ) and
acyl - CoA oxidase at both activity and mRNA levels ( 2.8 and 3.8 fold , respectively ) and enhances strongly
UCP1 mRNA level ( 9.5 fold ) during pre - hibernation . -
pubmed
The molecularly imprinted polymer (
MIP ) with good selectivity and affinity for
( -)- ephedrine was synthesized with
( -)- ephedrine as the template ,
methacrylic acid as the functional monomer . -
pubmed
This observation supports previous observations , suggesting that the elevation of
BNP in
cardiac amyloidosis may be due not only to elevated ventricular filling pressure but also to direct myocyte damage due to extracellular deposits of
amyloid . -
pubmed
MEASUREMENTS : Four measures derived from a stress -
strain - coping - support model of
alcohol , drugs and gambling problems and the family : Family Member Impact scale (
FMI ) , Symptom Rating Test ( SRT ) , Coping Questionnaire ( CQ ) , and Hopefulness - Hopelessness scale ( HOPE ) . -
pubmed
The
cupric ion as an inhibitor of photosynthetic electron transport in isolated
chloroplasts . -
pubmed
Binding of
Cu ( 2 + ) to the
chloroplast membranes resulted in removal of
Cu ( 2 + ) from solution . -
pubmed
Therefore , these findings suggest that enhanced
NRG1 signaling may contribute to
NMDA hypofunction in
schizophrenia . -
pubmed
Arachidonic acid can function as a signaling modulator by activating the
TRPM5 cation channel in taste receptor cells . -
pubmed
Intracellular
Ca ( 2 + ) is known to be a
TRPM5 activator , but the participation of lipid activators remains unreported . -
pubmed
To clarify the effect of
arachidonic acid on
TRPM5 in taste cells , we investigated the expression profile of a series of enzymes involved in controlling the intracellular free
arachidonic acid level , with the result that in a subset of taste bud cells ,
monoglyceride lipase (
MGL ) and
cyclooxygenase-2 (
COX-2 ) are expressed as well as the previously reported
group IIA phospholipase A ( 2 ) (
PLA ( 2 )- IIA ) . -
pubmed
These results strongly suggest the possibility that
arachidonic acid acts as a modulator of
TRPM5 in taste signaling pathways . -
pubmed
Obestatin is a recently discovered 23
amino acids peptide derived from the
ghrelin gene . -
pubmed
Moreover ,
obestatin administration had no effects on spontaneous
corticosterone secretion . -
pubmed
We have developed the computational models to analyze the deleterious nsSNPs in the transporters and predict ones in
ABCB (
ATP - binding cassette B ) transporters of interest . -
pubmed
EPO effects were similar to those of
memantine , a known neuroprotective agent . -
pubmed
IOP , in contrast , was unchanged by both
EPO and
memantine . -
pubmed
Blood
glucose , serum
insulin and
adiponectin levels were measured and mRNA for
angiotensinogen ,
angiotensin - converting enzyme and
angiotensin II type 1 receptor were determined in adipose tissue samples using RT PCR . -
pubmed
Cardiac surgery significantly increased both
insulin and blood
glucose levels suggesting the development of
insulin resistance , while serum
adiponectin levels did not change . -
pubmed
In hematologic oncology practice ,
troponin has been investigated extensively as a measure of
myocardial injury after therapy with radiation and
anthracyclines and after myeloablative therapies with stem cell replacement . -
pubmed
IMP preferring cytosolic
5 '- nucleotidase (
cN - II ) is an ubiquitous
nucleotide hydrolysing enzyme . -
pubmed
19 h later enzyme activity was decreased from 0.7+/-0.026 mU / mg in control
ADF cells to 0.45+/-0.046 mU / mg , while cell viability ( evaluated by the
MTT reduction assay ) decreased up to 0.59+/-0.01 ( fold vs control ) and
caspase 3 activity increased from 136+/-5.8 pmol min(-1 ) mg(-1 ) in control cells to 639+/-37.5 pmol min(-1 ) mg(-1 ) in silenced cells , thus demonstrating that
cN - II is essential for cell survival . -
pubmed
The results indicate that treatment with
xamoterol significantly reduced
PKA levels in the retinal endothelial cells cultured in high
glucose . -
pubmed
Blockade of
p42 /
p44 or
p38 signaling could restore
iNOS protein levels to those prior to
xamoterol stimulation , suggesting that
beta - adrenergic receptor regulates
iNOS protein levels through regulation of
PKA and
MAP kinase signaling . -
pubmed
Using a
copper sheet of 2.0 mm thickness or more prevented over - sensing of pacemakers in the present study , especially when serial X - ray exposures were used with an over - table fluoroscopy system or cone - beam
CT unit . -
pubmed
MAIN OUTCOME MEASURE(S ):
Glucose ,
triglycerides ,
high - density lipoprotein cholesterol ,
low - density lipoprotein cholesterol ,
DHEAS , total T ,
insulin , homeostatic model assessment for
insulin resistance (
HOMA - IR ) , and
visfatin levels were measured . -
pubmed
After therapy , statistically significant decreases in HOMA - IR , fasting
insulin , free T , and
DHEAS concentrations were observed for the
PCOS group . -
pubmed
Nicotine- and
methamphetamine - induced
dopamine release evaluated with in - vivo binding of
radiolabelled raclopride to
dopamine D2 receptors : comparison with in - vivo microdialysis data . -
pubmed
The effect of substances which alter extracellular
dopamine ( DA ) concentration has been studied by measuring changes in the binding of
radiolabelled raclopride , a
DA D2 receptor ligand that is sensitive to endogenous DA . -
pubmed
The apparent discrepancy in the extrasynaptic
DA -[ 3H ] raclopride binding relationship between the DA - enhancing drugs with and without
DAT - blocking property indicates that the competition between endogenous DA and
radiolabelled raclopride takes place at the intrasynaptic rather than extrasynaptic
DA D2 receptors and reflects synaptic concentration of DA . -
pubmed
In this study we identified the
GSK3 beta N - terminal
amino acids 78 - 92 as necessary for its association with
p53 . -
pubmed
Inhibitors of
GSK3 impaired the acetylation of
p53 at
Lys373 and
Lys382 near the
GSK3 beta binding region in
p53 , indicating that
GSK3 beta facilitates
p53 acetylation . -
pubmed
Cephalosporins ,
aminoglycosides ( usually
IV gentamicin ) ,
IV amoxicillin , and once
co - trimoxazole were recommended . -
pubmed
These results suggested that the timing of CBT with RIC may be very important , and cytoreduction with not only ASCT but also
bortezomib could give a promising chance for a successful
CBT . -
pubmed
Inhibition of oral
midazolam clearance by boosting doses of
ritonavir , and by
4,4 - dimethyl - benziso -( 2H )- selenazine (
ALT-2074 ) , an experimental catalytic mimic of
glutathione oxidase . -
pubmed
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT : * The
viral protease inhibitor
ritonavir is known to inhibit clearance of intravenous
midazolam . -
pubmed
* Therefore
ritonavir is an appropriate positive control inhibitor for clinical drug interaction studies involving
CYP3A substrates . -
pubmed
AIMS : We evaluated whether ' boosting ' doses of
ritonavir can serve as a positive control inhibitor for pharmacokinetic drug - drug interaction studies involving
cytochrome P450 3A (
CYP3A ) . -
pubmed
The study also determined whether
4,4 - dimethyl - benziso -( 2H )- selenazine (
ALT-2074 ) , an investigational
organoselenium compound that acts as a catalytic mimic of
glutathione oxidase , inhibits
CYP3A metabolism in vivo . -
pubmed
CONCLUSIONS : Low - dose
ritonavir produces extensive
CYP3A inhibition exceeding that of
ketoconazole ( typically 10- to 15 - fold
midazolam AUC enhancement ) , and is a suitable positive control index inhibitor for drug - drug interaction studies . -
pubmed
ALT-2074 inhibits
CYP3A metabolism to a small degree that is of uncertain clinical importance . -
pubmed
In addition , three
flavonoids (
kaempferol 3 - O - glycoside ,
apigenin-7 - O - rutinoside , and
naringenin ) were identified by using an LC -
MS /
MS apparatus . -
pubmed
MS2 titers were reduced by 1.14 log(10 ) PFU / ml with CJ , and 0.55 , 0.80 , and 0.96 log(10 ) PFU / ml with 0.15 , 0.30 , and 0.60 mg / ml
PAC , respectively . -
pubmed
INTERVENTION : We report 2 cases in which
n - BCA was used effectively as a salvage rescue in
aneurysms that ruptured during
coil embolization . -
pubmed
Finally a method for the surface treatment of Sylgard((R ) ) using
sulphuric acid and
fibronectin to enhance smooth muscle cell ( SMC ) adhesion is proposed and examined in vitro . -
pubmed
We compared the effects of
letrozole with that of
oxandrolone on predicted adult height (
PAH ) , puberty , bone mineral density , serum
insulin - like growth factor 1 ( IGF-1 ) and blood lipoproteins . -
pubmed
RESULTS :
Letrozole differed from
oxandrolone and placebo in significantly increasing
PAH ( p & lt ; 0.05 ) , and slightly but significantly decreasing HDL -
cholesterol . -
pubmed
The
G6PD enzyme catalyzes the first step in the
pentose phosphate pathway to protect cells against oxidative stress . -
pubmed
Replacing the
serine residue S621 of the
SGK consensus motif by an
alanine ( S621A ) abolished this stimulatory effect . -
pubmed
Following oxidative stress ,
DEDC sequentially decreased mitochondrial membrane potential ( DeltaPsim ) , reduced
Bcl-2 expression , increased
cytochrome c release , and activated
caspase-3 , - 8 , and - 9 . -
pubmed
Phosphorylation of
c - Jun N - terminal kinase (
JNK ) and
p38 mitogen activated protein kinase (
MAPK ) was stimulated by treatment with
DEDC . -
pubmed
To further investigate the mechanisms of the
DEDC - induced cell
death , we examined the effects of reactive
oxygen species scavenger
N - acetyl - L - cysteine (
NAC ) and selective inhibitors for
MAPK pathways on the cell
death . -
pubmed
The
DEDC - induced cell
death was significantly inhibited by both
NAC and
JNK inhibitor
SP600125 , but promoted by
p38 MAPK inhibitor ,
SB203580 . -
pubmed
Bcl - x ( L ) blocks a mitochondrial inner membrane channel and prevents
Ca2 + overload - mediated cell
death . -
pubmed
We have found that
Bcl - x ( L ) neuroprotective actions take place at mitochondria where this antiapoptotic protein delays both mitochondrial potential collapse and opening of the permeability transition pore by preventing
Ca ( 2 +)-mediated mitochondrial multiple conductance channel opening . -
pubmed
Bcl - x ( L ) neuroprotective actions were antagonized by the
Bcl - x ( L ) inhibitor
ABT-737 and potentiated by the
Ca ( 2 + ) chelator
BAPTA - AM . -
pubmed
The following general trends were observed : ( i ) order - order transitions from HEX Gyroid ( G ) Lamellae (
LAM ) ; ( ii ) the inter - cylinder spacing of the
HEX phase increased ; ( iii ) when the homopolymer molecular weight and concentration was high , macrophase separation of the system occurred . -
pubmed
A multiwalled
carbon nanotube ( MWCNT ) modified
glassy carbon electrode (
GCE ) is used for the simultaneous determination of
cefpirome by differential
pulse voltammetry and square wave voltammetry . -
pubmed
Results indicated that cathodic peak of
cefpirome is greatly improved at MWCNT modified
GC electrode as compared with the bare
GC electrode showing excellent electrocatalytic activity towards
cefpirome reduction . -
pubmed
Low
folate reduced overall methylation of
Slc394a by 3.4 % ( p=0.038 ) but did not affect
Esr1 or
Igf2 differentially methylated region (
DMR ) 1 . -
pubmed
Plasma lipids were not different between Strongmen and sedentary controls , but in comparison to runners , Strongmen had higher
low - density lipoprotein -
cholesterol ( p & lt ; 0.05 ) and lower
high - density lipoprotein cholesterol ( p & lt ; 0.01 ) . -
pubmed
The recent identification of a fourth
histamine receptor has reinforced clinical interest in the pleiotropic effects of
histamine and the relative roles of
histamine receptors in mediating immune and inflammatory responses . -
pubmed
MATERIAL AND METHODS : A comprehensive literature search was conducted for the following terms , alone or in combination :
allergic rhinitis ,
asthma , antihistamines ,
histamine , and
histamine receptors , and for the second - generation antihistamines
azelastine ,
cetirizine ,
desloratadine ,
ebastine ,
fexofenadine ,
levocetirizine ,
loratadine ,
mizolastine , and
rupatadine . -
pubmed
RESULTS : The study did not find a significant association between
alcohol - use disorders and HRQoL [ Physical Component Summary ( PCS ) and Mental health Component Summary (
MCS ) ] in this clinic population . -
pubmed
Exhaled
nitric oxide ( FeNO ) has been associated with
bronchial eosinophilia and with
airway hyperresponsiveness (
AHR ) in mild stable
asthma . -
pubmed
We previously demonstrated in a large project that allergen exposure is able to raise FeNO and to worsen
AHR to
bradykinin . -
pubmed
We postulated that allergen - induced increase in FeNO could be related to heightened mucosal eosinophils and
AHR to
bradykinin in
atopic asthma . -
pubmed
Inflammatory cell counts were related to FeNO and
AHR to
BK ( expressed as logPD20
bradykinin ) . -
pubmed
UNLABELLED : We studied the metabolic characteristics of
RCC subtypes and
angiomyolipoma with
18F - FDG and
11C - acetate PET /
CT . -
pubmed
Each lesion was assessed for SUVmax ratio ( lesion - to - normal kidney ) on
11C - acetate/
18F - FDG PET and attenuation density on
CT . -
pubmed
CONCLUSION :
11C - acetate PET /
CT helps in differentiating " fat - poor
angiomyolipoma " from
RCC . -
pubmed
RESULTS : There were no statistical differences concerning age , disease duration ,
L - Dopa equivalent dose , disease severity ( UPDRS III ) or dopaminergic uptake in
FP -
CIT - SPECT at baseline between both subgroups . -
pubmed
Using cell imaging , proteomics , and lipidomics , we show that
sFLT1 binds to the glycosphingolipid
GM3 in lipid rafts on the surface of podocytes , promoting adhesion and rapid
actin reorganization . -
pubmed
The solvent - free melt electrospinning (
MES ) method was developed to prepare a drug delivery system with fast release of
carvedilol (
CAR ) , a drug with poor water solubility . -
pubmed
The nature of cell
death was assessed by doubles - taining cells with
fluorescein isothiocyanate - labeled
Annexin V and
propidium iodide , and then analyzing the cells using flow cytometry . -
pubmed
Pretreatment with
propofol suppressed
H ( 2 ) O ( 2 )-induced
LDH release in a concentration - dependent manner , reducing the percentage of
LDH release to 38.1 + - 5.6 % , 33.5 + -
6.3 % , and 26.2 + - 3.8 % of the controls at 10 microM , 25 microM and 50 microM
propofol , respectively . -
pubmed
Numbers of viable cells increased following
propofol pretreatment , with 11.4 + - 10.9 % , 19.5 + -
16.1 % , and 32.4 + - 23.3 % cell survival rates after pretreatment with 10 microM , 25 microM and 50 microM
propofol , respectively . -
pubmed
These substitutions control hole delocalization in the QDs through the energetic alignment of the highest occupied orbitals of
PTC with the highest density - of - states region of the
CdSe valence band , to which
PTC couples selectively . -
pubmed
Antiplatelet therapy with
aspirin and a platelet
P2Y12 receptor antagonist reduces
thrombotic and
ischemic events after percutaneous coronary intervention and
acute coronary syndrome . -
pubmed
Radiosensitization of
prostate cancer cells by the dual
PI3K /
mTOR inhibitor
BEZ235 under normoxic and hypoxic conditions . -
pubmed
We investigated whether the novel
PI3K /
mTOR inhibitor
BEZ235 might sensitize
prostate cancer cells to radiation and reduce
hypoxia - induced radioresistance . -
pubmed
RESULTS :
BEZ235 rapidly inhibited
PI3 K and
mTOR signaling in a dose dependent manner and limited
tumor cell proliferation and clonogenic survival in both cell lines independently of
PTEN status . -
pubmed
Moreover ,
BEZ235 inhibited
PI3K /
mTOR activation and radiosensitized both cell lines under normoxia and
hypoxia . -
pubmed
BEZ235 radiosensitizing effects correlated with a decrease in gammaH2AX foci repair and increased
G2 / M cell cycle arrest . -
pubmed
Metformin also demonstrated a significant between - group advantage for
BMI ( -0.7 ; 95 % CI=-1.1 to -0.2 ) ,
triglyceride level ( -20.2 mg / dL ; 95 % CI=-39.2 to -1.3 ) , and
hemoglobin A1c level ( -0.07 % ; 95 % CI=-0.14 to -0.004 ) . -
pubmed
AIM : To assess whole - body
fluorodeoxyglucose (
FDG ) positron emission tomography / computed tomography ( PET /
CT ) in the management of
small bowel obstructions (
SBOs ) secondary to
gastric cancer and its role in treatment strategies . -
pubmed
Intravenous
paracetamol was found to be the most effective in reducing both blood pressure and heart rate ( p & lt ; 0.001).The effect on
post - operative
pain was insignificant . -
pubmed
By using interactive essential dynamics analysis , we found that
Galphas and
Gbetagamma domain of
Gs protein had the tendency to separate when the inverse agonist
ICI 118,551 bound to
beta2AR . -
pubmed
The alpha5 - helix had a relatively quick movement with respect to transmembrane segments of
beta2AR when the inverse agonist
ICI 118,551 bound to
beta2AR . -
pubmed
The present study focuses on the role of chitosan (
CS ) as nanofiller of
graphite oxide ( GO ) in order to prepare composite materials with improved
Hg ( II ) adsorption properties . -
pubmed
The removal of
Hg ( II ) from aqueous solutions was studied using adsorbents as
graphite oxide ( GO ) ,
graphite oxide nanofilled with chitosan ( GO /
CS ) and magnetic chitosan ( GO / mCS ) . -
pubmed
The zonation and structure of phototrophic microbial mats were studied along two thermal gradients in
sulfide - rich hot
springs of southwest Iceland . -
pubmed
Bisulfite sequencing analysis revealed the premature promoter demethylation of
Gata1 , a key myeloerythroid transcription factor and a
PIAS1 - target gene , in
Pias1(-/- ) HSCs . -
pubmed
He had a positive
In - pentetreotide indicating the presence of
somatostatin receptors . -
pubmed
H2O2 increased the density of nine
spots on two - dimensional gel electrophoresis of anti -
GSK-3beta - immunoprecipitates by more than 3 - fold . -
pubmed
The mitochondrial translocation of
GSK-3beta was attenuated also when
Lys-15 , but not
Arg-4 or
Arg-6 , in the N - terminal domain of
GSK-3beta was replaced with
alanine . -
pubmed
The oxidative stress - induced mitochondrial translocation of
GSK-3beta was associated with an increase in cell
death , which was suppressed by
lithium chloride (
LiCl ) , a
GSK-3beta inhibitor . -
pubmed
Comprehensive profiling of the reaction of
adenine propenal with
human serum albumin and the
DNA repair protein ,
XPA , revealed that the only stable adduct is
N ( epsilon )- oxopropenyllysine . -
pubmed
Modification by
adenine propenal dramatically reduces
XPA 's ability to bind to a DNA substrate . -
pubmed
The
GAD and
GABA - T enzyme activities were determined by measuring
GABA and
NADH production , respectively . -
pubmed
The
GAD enzyme inhibitor
semicarbazide (
SCZ ) blocked the sedative and hypnotic effects of B2 . -
pubmed
Multicentre randomised phase II trial of
gemcitabine+
platinum , with or without
trastuzumab , in advanced or metastatic
urothelial carcinoma overexpressing
Her2 . -
pubmed
CONCLUSION : The unexpectedly low incidence of
Her2 overexpression precluded the detection of a significant difference in efficacy on addition of
trastuzumab to
platinum - based chemotherapy with
gemcitabine . -
pubmed
Bosentan exhibited a positive exposure - adjusted signal in one assay (
BSEP inhibition ) and a moderate CVB body burden . -
pubmed
coli
strain , harbouring a gene cassette (
camA ( + )
camB ( + )
camC ) that encodes the F87W / Y96F / L244A / V247L mutant of
cytochrome P-450cam (
CYP101 ) , oxidised
HCB to
PCP . -
pubmed
P2X7 R - mediated
Ca ( 2 + ) -independent
d - serine release via
pannexin-1 of the
P2X7 R - pannexin-1 complex in astrocytes . -
pubmed
D - serine is a coagonist of
N - methyl - d - aspartate ( NMDA ) subtype of glutamate receptor and plays a role in regulating activity - dependent synaptic plasticity . -
pubmed
In this study , we examined the mechanism by which extracellular
ATP triggers the release of
d - serine from astrocytes and discovered a novel
Ca ( 2 + ) -independent release mechanism mediated by
P2X7 receptors (
P2X7 R ) . -
pubmed
Using
[ ( 3 ) H ] d - serine , which was loaded into astrocytes via the
neutral amino acid transporter 2 (
ASCT2 ) , we observed that
ATP and a potent
P2X7 R agonist ,
2 '( 3 ')- O -( 4 - benzoylbenzoyl ) adenosine-5 '- triphosphate (
BzATP ) , stimulated
[ ( 3 ) H ] D - serine release and that were abolished by
P2X7 R selective antagonists and by shRNAs , whereas enhanced by removal of intracellular or extracellular
Ca ( 2 + ) . -
pubmed
The
P2X7 R - mediated
d - serine release was inhibited by
pannexin-1 antagonists , such as
carbenoxolone (
CBX ) ,
probenecid (
PBN ) , and ( 10 )
Panx-1 peptide , and shRNAs , and stimulation of
P2X7 R induced
P2X7 R -
pannexin-1 complex formation . -
pubmed
Simply incubating astrocytes in
Ca ( 2 + ) /
Mg ( 2 + ) -free buffer also induced the complex formation , and that enhanced basal
d - serine release through
pannexin-1 . -
pubmed
The
P2X7 R - mediated
d - serine release assayed in
Ca ( 2 + ) /
Mg ( 2 + ) -free buffer was enhanced as well , and that was inhibited by
CBX . -
pubmed
Treating astrocytes with general
protein kinase C (
PKC ) inhibitors , such as
chelerythrine ,
GF109203X , and
staurosporine , but not
Ca ( 2 + ) -dependent
PKC inhibitor ,
Go6976 , inhibited the
P2X7 R - mediated
d - serine release . -
pubmed
Thus , we conclude that in astrocytes ,
P2X7 R -
pannexin-1 complex formation is crucial for
P2X7 R - mediated
d - serine release through
pannexin-1 hemichannel . -
pubmed
The release is
Ca ( 2 + ) -independent and regulates by a
Ca ( 2 + ) -independent
PKC . -
pubmed
The activated
P2X7 R per se is also functioned as a permeation channel to release
d - serine in part . -
pubmed
This
P2X7 R - mediated
d - serine release represents an important mechanism for activity - dependent neuron - glia interaction . -
pubmed
METHODS : Peripheral blood leukocyte and lymphocyte subsets , including
CD3(+ ) ,
CD4(+ ) , and
CD8(+ ) T cells ;
CD19(+ ) B cells ; and
CD56(+ ) natural killer ( NK ) cells , were obtained at baseline and monitored at 3 months , 6 months , and 12 months after initiation of
DMF treatment . -
pubmed
Employing this methodology , a number of
acylpolyamine spider toxins were synthesized and identified as venom components by UHPLC and ESI -
MS /
MS . -
pubmed
OBJECTIVE : To evaluate effects of holding parenteral nutrition (
PN ) for 3 hours prior to newborn screening (
NBS ) on false - positive
NBS rate for
amino acids ( AAs ) in very low
birth weight ( VLBW ) infants (
birth weight & lt;1500 g ) . -
pubmed
OBJECTIVE : In this study , a pilot - scale investigation was conducted to examine and compare the
biotoxicity of the organic compounds in effluents from five treatment processes (
P1 -
P5 ) where each process was combination of preoxidation (
O3 ) , coagulation , sedimentation , sand filtration , ozonation , granular activated
carbon , biological activated
carbon and chlorination (
NaClO ) . -
pubmed
Glycosylation inhibition reduces
cholesterol accumulation in
NPC1 protein - deficient cells . -
pubmed
We tested the hypothesis that
Niemann - Pick type C 1 (
NPC1 ) protein aids the transfer of low density lipoprotein - derived
cholesterol across this glycocalyx . -
pubmed
CRISPR generated ,
NPC1 - deficient ldl - D cells supplemented with
galactose accumulated more
cholesterol than those in which
sugar addition was blocked . -
pubmed
In the absence of
galactose supplementation ,
NPC1 - deficient ldl - D cells also transported more
cholesterol from lysosomes to the endoplasmic reticulum , as monitored by an increase in
cholesteryl [ ( 14 ) C ]- oleate levels . -
pubmed
These experiments support a model in which
NPC1 protein functions to transfer
cholesterol past a lysosomal glycocalyx . -
pubmed
These four compounds showed significant Ebola activity that is not specifically due to their inhibition of
S - adenonosylhomocysteine hydrolase , as might have been expected for
3 - deazaadenine carbocyclic nucleosides . -
pubmed
Synthesis , characterization , and H / D exchange of
mu - hydride - containing [
FeFe]-
hydrogenase subsite models formed by protonation reactions of
( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 (
TDT =
SCH2SCH2S ) with
protic acids . -
pubmed
As
[ FeFe ]- hydrogenase models , the first
thiodithiolate (
TDT ) ligand - containing
mu - hydride complexes
[ ( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 ( mu - H )](+) Y (- ) ( 2 - 7 , Y =
Cl ,
ClO4 ,
PF6 ,
BF4 ,
CF3CO2 ,
CF3SO3 ) have been prepared by protonation reactions of
( mu - TDT ) Fe2 ( CO ) 4 ( PMe3 ) 2 ( 1 ) with the corresponding
HY acids . -
pubmed
Cystatin C- and
creatinine - based estimation of
glomerular filtration rate ( eGFRcys and eGRFcreat , respectively ) and urinary
albumin /
creatinine ratio (
ACR ) were assessed to investigate their relationship with the risk of
atrial fibrillation . -
pubmed
Transient receptor potential ( TRP ) channels are recently identified proteins that form a versatile family of ion channels , the majority of which are
calcium permeable and exhibit complex regulatory patterns with sensitivity to multiple environmental factors . -
pubmed
RESULTS : Statins ,
proton pump inhibitors ,
angiotensin - converting enzyme inhibitors , and selective
serotonin reuptake inhibitors were determined to have class equivalence for efficacy , toxicity , and convenience . -
pubmed
Leuprolide acetate , a synthetic nonapeptide analogue of
gonadotropin - releasing hormone (
GnRH or
LHRH ) , is the active pharmaceutical ingredient used to treat a wide range of sex hormone - related disorders , including advanced
prostatic cancer ,
endometriosis , and
precocious puberty . -
pubmed
Association between
lipoxin A4 and
interleukin-12 in gingival crevicular fluid : a preliminary investigation . -
pubmed
This study aimed to explore the possibility of an association between
lipoxin A4 and
interleukin-12 in
chronic periodontitis . -
pubmed
Gingival crevicular fluid samples were collected and analysed for
interleukin-12 and
lipoxin A4 using ELISA . -
pubmed
There was a negative correlation between
interleukin-12 and
lipoxin A4 in both groups . -
pubmed
There was a negative correlation between clinical attachment loss and
lipoxin A4 , and a positive correlation between clinical attachment loss and
interleukin-12 . -
pubmed
Lipoxin A4 possibly has an inhibitory effect on
interleukin-12 . -
pubmed
An
FDG PET /
CT scan was performed to evaluate the whole body in November 2015 . -
pubmed
MATERIALS AND METHODS : The SRP 68 01 gamma spectrometer with attached scintillation detector based on a
NaI ( Tl ) O 30 x 25 mm2 crystal and
NC 482 VM one channel spectrometer ( Bicron Co. ) with attached scintillation detector based on a
NaI ( Tl ) O 52 x 52 mm2 crystal were used for the study . -
pubmed
It Remains Unknown Whether
Filaggrin Gene Mutations Evolved to Increase Cutaneous Synthesis of
Vitamin D. About 8 - 10 % of normal Northern Europeans are heterozygous carriers of common
FLG mutations , while only 1 - 4 % of southern Europeans display these mutations , and only very rarely are mutations detected in African populations . -
pubmed
Importantly ,
FLG mutation carriers have 10 % increased serum
vitamin D concentrations compared to controls . -
pubmed
Based on these observations , we have proposed that this latitude - dependent gradient of
FLG mutations across Europe , Asia and Africa could have provided an evolutionary advantage for heterozygous
FLG mutation carriers , residing at northern latitudes , depletion of the
FLG downstream product ,
trans - urocanic acid , would facilitate the intracutaneous synthesis of
vitamin D3 by allowing increased transcutaneous absorption of UVB photons . -
pubmed
Proteome analysis of irradiated endothelial cells reveals persistent alteration in protein degradation and the
RhoGDI and
NO signalling pathways . -
pubmed
RESULTS : The radiation - induced alteration of the endothelial proteome was characterized by sustained perturbation of
Rho GDP - dissociation inhibitor (
RhoGDI ) and
nitric oxide (
NO ) signalling pathways . -
pubmed
The cumulative number of alleles associated with lower
DHEAS within genes
ZKSCAN5 ,
SULT2A1 ,
HHEX , and
ARPC1A present in each individual negatively correlated with
DHEAS levels in
RA patients , but not in controls . -
pubmed
Linear regression analysis showed significant effect of polymorphisms in genes
ZKSCAN5 and
ARPC1A on serum
DHEAS levels in female
RA patients but not in the control group . -
pubmed
Urate promotes
SNCA /
alpha - synuclein clearance via regulating
mTOR - dependent macroautophagy . -
pubmed
Moreover , UA was still able to increase the
LC3 - II level and the number of
LC3 puncta in the presence of
Bafilomycin A1 , a lysosomal inhibitor . -
pubmed
Co - treatment with
3 - benzyl-5 -(( 2 - nitrophenoxy ) methyl )- dihydrofuran-2 ( 3H )- one (
3BDO ) , an
mTOR activator , abolished the UA - induced
LC3 - II increase . -
pubmed
METHODS : A total of 157
HIV - infected patients were screened through standard serological tests , including Wright 's test , Coombs ' Wright test , and
2 - mercaptoethanol Brucella agglutination test (
2ME test ) , blood cultures in Castaneda media , and
CD4 counting . -
pubmed
Furthermore , the
LsEm1 gene was also expressed in response to salt ,
dehydration ,
abscisic acid ( ABA ) , and cold stresses in young seedlings . -
pubmed
ALRN-6924 , a stapled peptide that blocks interactions between
p53 and both
MDM2 and
MDMX has potent in vitro activity and superior in vivo activity across 8 different
PDX models compared to the standard - of - care agent
romidepsin . -
pubmed
We prepared the photosensitizer conjugates by expressing a recombinant Fab fragment and specifically linking IR700 -
maleimide at the C - terminal of the
Fab heavy chain . -
pubmed
Exogenous application of the
ethylene precursor
1 - aminocyclopropane-1 - carboxylic acid (
ACC ) was found to promote the emergence of
nodal roots . -
pubmed
Transcriptome analysis of
nodal tissues revealed that the expression of genes involved in metabolic processes and cell wall biogenesis was upregulated in response to
ACC treatment , supporting the notion that
ethylene is a positive regulator for the outgrowth of young root primordia . -
pubmed
In BSV::
ARGOS8 transgenic plants with reduced
ethylene sensitivity due to constitutive overexpression of
ARGOS8 ,
nodal root emergence was delayed and the promotional effect of
ACC on
nodal root emergence decreased . -
pubmed
This finding reveals how
GARP exploits an unusual medley of interactions , including fold complementation by the
amino terminus of
TGF - beta1 , to chaperone and orient the
cytokine for binding and activation by
alphaVbeta8 . -
pubmed
The electrical performance of the grown
NiSi /
SiC core - shell nanowires was characterized by the conducting
AFM and it is found that the measured conductivities of the nanowires were higher than the reported works that might be enhanced by
SiC shell layer on
NiSi nanowires . -
pubmed
Copanlisib : An Intravenous
Phosphatidylinositol 3 - Kinase (
PI3 K ) Inhibitor for the Treatment of
Relapsed Follicular Lymphoma . -
pubmed
Data Sources : Sources of information were identified through searches of PubMed ( August 2014 to January 2019 ) using the key terms
copanlisib ,
Aliqopa ,
PI3 K inhibitor , and
BAY 80 - 6946 . -
pubmed
The safety and efficacy of
copanlisib has been studied in the multicenter , single - arm , phase
II CHRONOS-1 study . -
pubmed
Angiotensin II (
ATII ) is a
noncatecholamine vasopressor recently approved for the management of distributive
shock , but limited data support its clinical utility . -
pubmed
To evaluate bone metabolism , markers (
alkaline phosphatase and
parathyroid hormone - PTH ) and related nutrients (
vitamin D ,
vitamin B12 ,
calcium ,
phosphorus ,
magnesium ,
potassium and
zinc ) were analyzed . -
pubmed
After logistic regression , it was observed that inadequacies of
calcium ( OR : 4.11 ; 95 % CI : 2.33 - 6.66 ) ,
phosphorus ( OR : 3.03 ; 95 % CI : 1.98 - 5.79 ) ,
vitamin D ( OR : 5.01 ; 95 % CI : 2.92 - 6.71 ) and
PTH ( OR : 5.45 ; 95 % CI : 4.49 - 6.74 ) were significantly higher in the
MUHO group compared to the MHO Group . -
pubmed
Following resonance - enhanced multiphoton ionisation via the 11pipi * and 11pisigma * states of
phenol , we observe 11pipi*-D0 /
D1 ionisation and competing direct S0 - D0 /
D1 ionisation . -
pubmed
We also present a computational strategy for calculating vertical ionisation energies using a quantum - mechanics / effective fragmentation potential ( QM /
EFP ) approach , in which we find that optimising the configurations obtained from molecular dynamics simulations and using the
[ phenol ( H2O ) 5 ] QM [( H2O ) n>=250]EFP ( B3LYP / aug - cc - pvdz ) method gives good agreement with experiment . -
pubmed
A composite chitosan / nano - activated
carbon (
CS -
NAC ) aminated by
( 3 - aminopropyl ) triethoxysilane (
APTES ) was prepared in the form of beads and applied for the removal of
acetaminophen from aqueous solutions . -
pubmed
The aminated adsorbent (
CS -
NAC - APTES beads ) prepared with 40 % w / w
NAC and 2 % v / v
APTES showed higher adsorption capacity ( 407.83 mg / g ) than
CS -
NAC beads ( 278.4 mg / g ) . -
pubmed
Brunauer - Emmett - Teller ( BET ) analysis demonstrated that the surface area of the
CS - NAC - APTES beads was larger than that of
CS -
NAC beads ( 1.16 times ) . -
pubmed
To demonstrate applications of this CRISPR -
dCpf1 system in metabolic engineering , we applied this system to repress four genes involved in
lysine biosynthesis (
gltA ,
pck ,
pgi , and
hom ) with a single array , which increased the
lysine titer and yield for over 4.0 - fold . -
pubmed
Fucoidan , a ligand for the
homing molecule l - selectin (
CD62L ) , is known to induce rapid up - regulation of several
chemokine receptors on lymphocytes . -
pubmed
Transcriptional Induction of
Cystathionine gamma - Lyase , a Reactive
Sulfur - Producing Enzyme , by
Copper Diethyldithiocarbamate in Cultured Vascular Endothelial Cells . -
pubmed
Reactive
sulfur species are produced by enzymes , such as
cystathionine gamma - lyase (
CSE ) ,
cystathionine beta - synthase ,
3 - mercaptopyruvate sulfurtransferase , and
cysteinyl - tRNA synthetase . -
pubmed
Cu10 selectively upregulated
CSE gene expression in vascular endothelial cells independent of cell density . -
pubmed
This transcriptional induction of endothelial
CSE required both the
diethyldithiocarbamate scaffold and the coordinated
copper ion . -
pubmed
Additionally , the present study revealed that
ERK1 / 2 ,
p38 MAPK , and
hypoxia - inducible factor ( HIF )- 1alpha /
HIF-1beta pathways mediate transcriptional induction of
endothelial CSE by
Cu10 . -
pubmed
The transcription factors
NF - kappaB ,
Sp1 , and
ATF4 were suggested to act in constitutive
CSE expression , although the possibility that they are involved in the CSE induction by
Cu10 can not be excluded . -
pubmed
The present study used a
copper complex as a molecular probe to reveal that the transcription of
CSE is regulated by multiple pathways in vascular endothelial cells , including
ERK1 / 2 ,
p38 MAPK , and
HIF-1alpha /
HIF-1beta . -
pubmed
Certain antiviral drugs such as
Remdesivir are
RNA dependent RNA polymerase inhibitor . -
pubmed
RESULTS : It is reported that
ATP is involved in synthesis of coronavirus non - structural proteins from
3CLpro and
PLpro . -
pubmed
Protein adsorption studies showed the
PEUs did not led to significant platelet adsorption in platelet rich plasma (
PRP ) after pretreatment with
fibrinogen . -
pubmed
Release of
human chorionic somatomammotrophin from isolated perfused lobules and superfused fragments of term placenta : spontaneous liberation and the effects of cholinergic drugs ,
dibutyrylcyclic adenosine monophosphate and
calcium . -
pubmed
Single isolated lobules from term placentae were bilaterally perfused with Earle 's
solution , and the release of
human chorionic somatomammotrophin (
HCS ) was measured by radioimmunoassay . -
pubmed
The ability of the placenta to respond to alterations in the perfusion environment was verified by stimulating the
HCS secretion with 1 mM
dibutyryl cyclic adenosine monophosphate and by modifying
calcium concentrations . -
pubmed
High
calcium ( 10 mM
CaCl2 ) led to a sustained increase of
HCS release . -
pubmed
Three criteria of enzyme activity were compared : A , total radioactivity of the washed protein precipitate ; B , this figure minus activity incorporated in the absence of the
collagen acceptor ; and C , radioactivity incorporated into the mixed
amino acid fraction , collected by elution with dilute
NH4OH from a
Dowex 50 resin column after alkaline hydrolysis of the
protein . -
pubmed
The ratio of
EGOT with in vitro stimulation by
PLP to EGOT actual (
alpha - EGOT ) was also studied . -
pubmed
Prostaglandin E2 has previously been shown to enhance the shape transformation of sickle prone erythrocytes ( 8) and to reduce the
oxygen resaturation of
Hemoglobin SS within intact sickle cell erythrocytes after deoxygenation ( 15 ) . -
pubmed
The involvement of active
oxygen species , such as singlet
oxygen and
H2O2 , was established by the inhibition of DNA breakage by
catalase and by
sodium azide . -
pubmed
To elucidate a possible mechanism for the inhibition of
AS101 , we have analyzed the effect of the drug on the catalytic functions associated with HIV
RT , namely the RDDP , DDDP , and
RNase H activities . -
pubmed
On the other hand , the
RNase H activity was less sensitive to
AS101 , with an apparent IC50 value of about 30 microM. -
pubmed
The ability of
IL-10 and
TGF - beta to suppress microbicidal function was also positively correlated with inhibition of
nitrite generation in macrophage culture supernatants . -
pubmed
These and the deduced
amino acid sequences were compared with those of a subtype
O1 virus
strain . -
pubmed
VP4 and
VP2 differed by 2.4 and 1.8 % , whereas
VP1 , known as major viral
antigen , and
VP3 differed by 8 % and 5.5 % , respectively . -
pubmed
The
glucose - phosphorylating enzyme
glucokinase likely plays an important role in regulating
glucose - stimulated
insulin secretion from the islets of Langerhans and has previously been thought to be expressed only in that tissue and in liver . -
pubmed
AtT20ins cells , in contrast , exhibited no response to
glucose at any concentration tested , despite the fact that
insulin secretion from both cell lines was stimulated by incubation with
dibutyryl cAMP . -
pubmed
The intensity of the
TMAO resonance for
CRF subjects was correlated with the plasma concentration of
urea ( R = 0.55 ) and
creatinine ( R = 0.74 ) , suggesting that the presence of
TMAO is closely related to the degree of
renal failure . -
pubmed
Among individual phenomena the following are important : in stage I regular contact with the
drug ( 04 ) and increased tolerance ( 05 ) , in stage
II secret drinking ( 2 ) and frequency of palimpsests ( 7 ) , in stage III signs derived mostly from rationalization and
alcohol - centered behaviour and finally in stage
IV impaired thinking ( 33 ) , reduced tolerance ( 37 ) and possible ethic degradation ( 32 ) . -
pubmed
The level of
glucokinase activity does not appear to be responsible for poor
glucose utilization in liver . -
pubmed
The binding of
palmitate to the
protein was saturable . -
pubmed
In contrast , the activity of
His - tRNA synthetase was significantly increased in the symptomatic phase of
MeHg intoxication . -
pubmed
The activities of these six
aminoacyl - tRNA synthetases in the control brains were affected to different extents on the direct addition of
MeHg to the assay system in vitro . -
pubmed
The chief advantages over conventional methods include virtually no loss of leukocytes during washing and separation steps ; a significant reduction in the time required to prepare leukocytes for radiolabeling compared to non - hemolytic preparations ; a 28 % increase in the average labeling efficiency obtained using
111In - oxine ; greater than 95 % cell viability as measured by the
trypan blue exclusion test ; elimination of contaminating red blood cells from the leukocyte pellet prior to labeling ; and 80 % survivability at 15 min
post injection ( measured as per cent of blood activity on leukocyte fraction ) . -
pubmed
Inhibition of myogenesis by
5 - bromodeoxyuridine or by
calcium deprivation prevents the developmentally associated changes in the expression of
H36 . -
pubmed
We conclude that
H36 is a muscle - specific , developmentally regulated cell - surface
antigen that may have a role in myoblast differentiation and that can be used to determine the embryonic lineages of skeletal and cardiac muscle . -
pubmed
IRBP thus appears to be a unique , conserved glycoprotein of the retinal extracellular matrix that could serve as a
retinoid - transport vehicle . -
pubmed
The influence of
triamcinolone upon the content of
antidiuretic hormone in the hypothalamus and its release from the hypophysis into blood . -
pubmed
Increased release of
LH after administration of
LH - RH to men pretreated with
clomiphene . -
pubmed
Among the various cell types , the extent of
[ 3H ] PDBu binding did not correlate with the extent of
EGF binding , indicating that these two substances occupy distinctly separate types of receptors . -
pubmed
If present in sufficient amounts ,
aromatase could catalyze the synthesis of
estrogens from plasma
steroid precursors and produce high
breast cancer tissue concentrations . -
pubmed
To determine the biological importance of
tumor aromatase , we validated a specific and highly sensitive
3H - labeled water release assay for
aromatase and used this to quantitate the amount of
estrogen synthesized in vitro in
breast tumors . -
pubmed
h. Three
aromatase inhibitors (
aminoglutethimide ,
testololactone , and
4 - hydroxyandrostenedione ) blocked this activity at concentrations similar to those affecting
aromatase activity in other tissues . -
pubmed
If biologically important , the
estrogen formed locally from
aromatase would be expected to stimulate production of the
progesterone receptor . -
pubmed
Under these circumstances , a positive correlation of
progesterone receptor and local
estrogen production should be found . -
pubmed
Lack of effect of
domperidone on
gastrin release : evidence for a peripheral activity of the drug . -
pubmed
The lack of effect on
gastrin release appears to confirm that
domperidone is unable to penetrate the blood brain barrier in appreciable amounts in adults . -
pubmed
In addition a portion of the enriched NK cells were positive for
OKM1 ,
asialo - GM1 , and Ia
antigens , which are undetectable on typical T cells but are present on PMN and/or monocytes . -
pubmed
Zinc levels and , hence ,
SCL : SZL ratios did not reflect
tumor activity . -
pubmed
Vitronectin receptor expression and distribution at the photoreceptor - retinal
pigment epithelial interface . -
pubmed
A double - labeling procedure , employing
VnR antibody and a fluorescently labeled phallotoxin (
phalloidin ) , was used to identify regions of
VnR co - distribution with filamentous
actin (
F - actin ) . -
pubmed
Intravaginal
clindamycin use caused a decrease 1 week
post - therapy , but at 1 month , levels of lactobacilli were similar to those in the
metronidazole treatment group . -
pubmed
The intracellular localization of
Cu / Zn - and Mn - superoxide dismutase (
SOD ) , which catalyze the dismutation of
superoxide radicals (
O2- ) to
O2 and
H2O2 , was studied in the thyroid tissue of various
thyroid disorders by an immunohistochemical technique . -
pubmed
The concentrations of
Cu /
Zn - and
Mn - SOD in thyroid
tumor tissues and
hyperplastic follicular disorders were significantly higher than those in
normal thyroid tissue when they were compared as a function of protein or deoxyribonucleic acid contents . -
pubmed
13C NMR relaxation data have been used to determine dipolar auto- and cross - correlation times for the di- and
tripeptides GK , KG , and GKG , primarily to analyze
lysine side - chain motional dynamics . -
pubmed
Morphogenetic effect of
kainate on adult hippocampal neurons associated with a prolonged expression of
brain - derived neurotrophic factor . -
pubmed
Therefore
CA 19 - 9 and
Sialyl Le ( x ) are
tumor - associated antigens in the whole colorectum , and
Leb and
Le ( y ) in the distal colon . -
pubmed
Expression of
CA 19 - 9 in primary
tumor provided more prognostic information and risk of
liver metastasis than other Lewis blood group
antigens . -
pubmed
Exocytosis in neutrophils induced by
thapsigargin and other inhibitors of
calcium ATP - ase . -
pubmed
Fluoride , a non - specific inhibitor of
Ca ( 2 +)- ATPase , also causes exocytosis . -
pubmed
Thapsigargin - induced exocytosis is not inhibited by L - type channel antagonists like
verapamil , but is strongly inhibited by
lanthanum ions , suggesting that the
calcium required for induction of exocytosis is not entering via L - type channels , but via
La ( 3 +)- sensitive calcium channels . -
pubmed
The
NGFr ( p75 )- IR identified with the
electron microscope was located within small thinly myelinated and unmyelinated axons and within axon terminals . -
pubmed
The profiles with
NGFr ( p75 )- IR seen with the
electron microscope indicate a primary afferent origin and show some similarities when compared to other markers of primary afferent fibers such as
calcitonin gene - related peptide . -
pubmed
In particular central catecholaminergic neurones and
alpha - adrenoceptors have been analysed in detail , and they are recognized as important targets of the classic centrally acting antihypertensives
clonidine ,
guanfacine and
alpha - methyl - DOPA . -
pubmed
Initially these drugs have been assumed to reduce elevated blood pressure via the stimulation of central
alpha 2 - adrenoceptors in the brain stem , thus leading to peripheral sympathoinhibition and a reduction of elevated blood pressure , heart rate and plasma
catecholamines . -
pubmed
Finally , the peripheral alpha 1 - blocker
urapidil has been demonstrated to possess an additional central mechanism , mediated by the stimulation of
serotonergic 5HT1A - receptors located in the rostral ventrolateral medulla . -
pubmed
The
serotonin 5 - HT3 receptor is unique among the seven
serotonin receptor " families " that have been recognized so far . -
pubmed
Tyrosine phosphorylation was similar among MRL and normal
CD4 + T cells after cross - linking with either
anti - TCR antibody or
anti - CD3 antibody , and following co - culture with Con A. -
pubmed
In constrast , cross - linking of surface
CD4 resulted in deficient
tyrosine phosphorylation of
cellular proteins in MRL T cells . -
pubmed
The
gL proteins of alpha herpesviruses and gamma herpesviruses do not have a significant percentage of
amino acid sequence homology . -
pubmed
Inhibition of
dipeptidyl peptidase IV (
CD26 ) by peptide
boronic acid dipeptides . -
pubmed
Rate constants were determined for the inhibition of
DPPIV using several peptide
boronates at different pH values . -
pubmed
Val - boroPro forms the most tightly bound complex with
DPPIV ; the first order half life for dissociation of the inactive enzyme - inhibitor complex at 23 degrees C is approximately 27 days . -
pubmed
VES , although having little effect on
E2F-1 steady - state protein levels , decreased
E2F-1 phosphorylation and transactivation activity and increased
cyclin A binding to
E2F-1 . -
pubmed
The functional consequence of the negative regulatory effect of
VES on
E2F-1 function was shown by the ability of
VES to inhibit the transcriptional activation of an
E2F-1 responsive gene ,
c - myc . -
pubmed
Anti - platelet action of
nitric oxide and selective
phosphodiesterase inhibitors . -
pubmed
Since the
NO effect is mediated via increased
cGMP , this in vitro study was undertaken to test the hypothesis that selective
phosphodiesterase (
PDE ) inhibitors might enhance aggregation inhibition at lower
NO concentrations . -
pubmed
Because the
cAMP - selective
PDE III and the
cGMP - selective
PDE V are prominent in platelets ,
milrinone , a
PDE III inhibitor , and
zaprinast , a
PDE V inhibitor , were tested alone and in the presence of
NO for their effect on aggregation . -
pubmed
, formation of lipid
peroxides , enlargement of HDL diameters , an increased exposure of the
tryptophan groups of the
apolipoprotein A - I to a more hydrophilic environment , formation of
bityrosines , and cross - linking of
apolipoprotein A - I. -
pubmed
When these treatments were performed in the absence of
EDTA , most of the modifications described above were more intense and
HDLs formed a macroaggregate that displays a rosette - like structure . -
pubmed
Native
HDL or
HDLs treated in the presence of
EDTA inhibited the neuritogenesis of undifferentiated neurons but did not show any significant effect on the differentiated neurons in culture . -
pubmed
Characterization of
human IgG1 monoclonal antibody against
gangliosides expressed on
tumor cells . -
pubmed
Despite a decrease in blood pressure ,
minoxidil had no effect on arterial weight or
collagen content but increased
elastin content in the abdominal aorta , renal , and superior mesenteric arteries . -
pubmed
Thus the
minoxidil - induced increase in arterial
elastin content may be related to a direct effect of the drug to decrease
elastase activity in these tissues . -
pubmed
Although the nucleosome structure was formed as revealed by the
micrococcal nuclease digestion , the
B - type lamin uptake into the nuclei was inhibited by
hemin . -
pubmed
Some of the
hemin - binding proteins bound concanavalin A (
Con A ) . -
pubmed
Autoantibodies to
steroid 21 - hydroxylase ,
17alpha - hydroxylase , and
P450 side chain cleavage enzyme were measured by immunoprecipitation assay using
35S - labeled recombinant proteins . -
pubmed
Over recent years , a number of
imidazole derivatives that specifically bind to the
angiotensin II type 1 receptor , thereafter called sartans , have been developed and made available to the clinician . -
pubmed
The results showed that
Ca ( 2 +)- ATPase does not cause significant alterations in the interdigitation of 16:0
LPC /
DPPC ( 27.0 mol%
LPC ) vesicles when it is reconstituted with lipids . -
pubmed
Intrinsic fluorescence ,
acrylodan fluorescent adducts , and CD spectra indicated that
Ca ( 2 +)- ATPase , when embedded in interdigitated bilayer structures , is more exposed to the hydrophilic environment and has a looser structure than when embedded in non - interdigitated bilayers . -
pubmed
Intracellular
cholesterol biosynthesis may play a key role in supplying
cholesterol ( as
cholesteryl ester ) for the neutral core of
very low density lipoprotein (
VLDL ) , thus modulating the secretion of
apolipoprotein B-100 (
apo B-100 ) from hepatocytes . -
pubmed
Pulse chase experiment demonstrated that
NK-104 did not affect the synthetic rate of
apo B-100 but increased intracellular degradation of newly synthesized protein . -
pubmed
Experiments using
cytochalasin A as an anti - cytoskeletal drug revealed that a functional
actin cytoskeleton is crucial for mitochondrial morphology and the dynamic behavior of the mitochondrial network . -
pubmed
Structure of the metal - water complex in
Ras x GDP studied by high - field
EPR spectroscopy and
31P NMR spectroscopy . -
pubmed
On
Mg ( 2 + ) --> ;
Mn ( 2 + ) substituted samples , the first ligand sphere of the metal ion in the inactive ,
GDP - bound
Ras has been studied by continuous wave
EPR at 94 GHz ( W - band ) . -
pubmed
However , for the
alanine mutant in position 35
Ras(T35A ) as well as for the oncogenic mutant
Ras(G12V ) , four water ligands can be observed in liquid solution . -
pubmed
The
EPR studies were supplemented by
( 31 ) P NMR studies on the
Mg ( 2 + ) x
GDP complexes of the wild - type protein and the three mutants . -
pubmed
RESULTS :
Cannabinoids were more effective antiemetics than
prochlorperazine ,
metoclopramide ,
chlorpromazine ,
thiethylperazine ,
haloperidol ,
domperidone , or
alizapride : relative risk 1.38 ( 95 % confidence interval 1.18 to 1.62 ) , number needed to treat 6 for complete control of
nausea ; 1.28 ( 1.08 to 1.51 ) ,
NNT 8 for complete control of
vomiting . -
pubmed
Some potentially beneficial side effects occurred more often with
cannabinoids : " high " 10.6 ( 6.86 to 16.5 ) ,
NNT 3 ; sedation or
drowsiness 1.66 ( 1.46 to 1.89 ) ,
NNT 5 ;
euphoria 12.5 ( 3.00 to 52.1 ) ,
NNT 7 . -
pubmed
Harmful side effects also occurred more often with
cannabinoids :
dizziness 2.97 ( 2.31 to 3.83 ) ,
NNT 3 ;
dysphoria or
depression 8.06 ( 3.38 to 19.2 ) ,
NNT 8 ;
hallucinations 6.10 ( 2.41 to 15.4 ) ,
NNT 17 ;
paranoia 8.58 ( 6.38 to 11.5 ) ,
NNT 20 ; and
arterial hypotension 2.23 ( 1.75 to 2.83 ) ,
NNT 7 . -
pubmed
Second ,
Delta5 desaturase was more sensitive than
Delta6 desaturase to changes in
alcohol concentration . -
pubmed
Third , in SHR , a tremendous decrease of
arachidonic acid biosynthesis was evidenced in
alcohol - intoxicated hepatocytes ; the effect was reinforced when
ethanol concentration was high , mainly for
Delta5 desaturase . -
pubmed
METHODS : In situ hybridization with
digoxigenin RNA probes localized cells responsible for
gelatinases A and B ,
TIMP-1 and
TIMP-2 mRNA production in histologic sections of aneurysmal ( n = 12 ) , and normal ( n = 6 ) cerebral arteries . -
pubmed
Interferon - alpha-2b and
ribavirin for retreatment of
chronic hepatitis C. -
pubmed
The physical interaction of
HIF-2alpha with
Ets-1 was demonstrated and localized to the
HIF-2alpha carboxyl terminus and the autoinhibitory exon VII domain of
Ets-1 , respectively . -
pubmed
The deletion of the DNA binding and
carboxyl - terminal transactivation domains of
HIF-2alpha , respectively , created dominant negative mutants that suppressed transactivation by the wild type protein and failed to synergize with
Ets-1 . -
pubmed
Graft survival correlated with high dose
cyclosporine treatment , complete complement blockade and the absence of an induced
PAMA response to the
murine anti - C5 monoclonal antibodies . -
pubmed
An antagonist (
AM251 ) of the
type-1 cannabinoid receptor blocked the depolarization - induced suppression of evoked inhibitory postsynaptic currents ( eIPSCs ) , and the cannabinoid
WIN55,212 - 2 reduced eIPSC amplitude and occluded suppression . -
pubmed
The degree of
WIN55,212 - 2 - mediated inhibition of eIPSCs was strongly correlated with the magnitude of depolarization - induced suppression of the eIPSCs , suggesting that the
WIN - sensitive afferents are suppressed by
PN depolarization . -
pubmed
The
beta - site amyloid precursor protein cleaving enzyme 1 (
BACE1 ) , an integral membrane
aspartyl protease responsible for cleavage of
amyloid precursor protein (
APP ) at the beta - site , promotes
A beta production . -
pubmed
To analyze the protective effects of the
aminotetraline derivative
( + /-)-( R , S )- 5,6 - dihydroxy-2 - methylamino-1,2,3,4 - tetrahydro - naphthalene hydrochloride (
CHF-1024 ) , a compound endowed with
DA2 - dopaminergic /
alpha2 - adrenergic receptor agonistic activity , in
myocardial ischemia /
reperfusion damage . -
pubmed
This anti -
ischemic effect of
CHF-1024 was associated to a decrease in
creatine kinase and
lactate dehydrogenase , both released during heart reperfusion . -
pubmed
These events were concomitant with maintenance of a higher production of
6 - keto - prostaglandin F1alpha The ability of
CHF-1024 to improve postischemic
ventricular dysfunction was correlated with a dose - dependent inhibition of the release of both
norepinephrine ( NE ) , from sympathetic nerve endings , and
TNF - alpha from cardiac tissue . -
pubmed
The action of
insulin on the penetration of
sugars into the perfused heart . -
pubmed
Olopatadine also significantly reduced
ICAM-1 expression compared with placebo at 30 minutes and 5 hours postchallenge(P & lt ; 0.03 and P & lt ; 0.01 , respectively ) . -
pubmed
Platelet binding of
Stx was significantly higher in
EDTA - washed preparations relative to ACD - derived platelets . -
pubmed
Binding of
Stx was also increased with ACD - derived platelets when activated with
thrombin ( 1 U mL-1 ) and exposure of the
Gb3 Stx receptor was detected only on platelets subjected to
EDTA , higher g forces or
thrombin . -
pubmed
P - selectin (
CD62P ) exposure was significantly increased in
EDTA - washed preparations relative to ACD - derived platelets , suggesting platelet activation . -
pubmed
This study investigated apoptosis in
prostate cancer before and after neoadjuvant treatment with
LH - RH analog , demonstrating that this therapy induced high
AI and
bax and
survivin overexpression , thus acting as a proapoptotic agent in
prostate cancer . -
pubmed
Here we test the hypothesis that
glutaredoxin-1 (
Grx-1 ) , a member of the
oxidoreductase family of enzymes , may be a critical component of redox - sensitive molecular switches by mediating reversible
protein S - glutathionylation and enzymatic catalysis of
thiol /
disulfide exchange . -
pubmed
ATP did not effect the chaperone activity of
Hsc70 - SG but did increase the activity of reduced
Hsc70 - SG . -
pubmed
OBJECTIVE : Cardiac
Troponin I (
cTnI ) phosphorylation by
protein kinase C (
PKC ) results in a reduction of maximal
actomyosin ATPase activity , an effect that is more marked at higher levels of
calcium (
Ca2 + ) and is likely to reduce active force development . -
pubmed
Thus ,
PKC phosphorylation sites on
cTnI play a role in attenuating contractile responses to changes in intracellular
Ca2 + . -
pubmed
Moreover ,
plc1 appeared paler than the wild type , the result of an altered differentiation of chloroplasts and reduced
chlorophyll levels compared with wild type filaments . -
pubmed
In
PJS patients ,
COX-2 was inhibited with a daily dose of 2 x 200 mg
celecoxib for 6 months . -
pubmed
We show that intra - LA infusions of the
MAPK kinase (
MEK ) inhibitor
U0126 , a manipulation which inhibits activation of
ERK -
MAPK , impairs postreactivation long - term memory ( PR - LTM ) but leaves the postreactivation short - term memory (
PR -
STM ) intact . -
pubmed
Post - reactivation infusions of
cycloheximide blocked
PR - LTM but not
PR -
STM and , in the absence of reactivation , had no effect . -
pubmed
Resistance to
thyroid hormone in a Chinese family with R429Q mutation in the
thyroid hormone receptor beta gene . -
pubmed
Diuretics ,
calcium antagonists ,
ACE inhibitors and
angiotensin receptor blockers ( ARB ) are equally suitable for the reduction of blood pressure after apoplectic insult . -
pubmed
The direct
thrombin inhibitor ,
ximelagatran , will be available as an alternative to the oral anticoagulant marcumar in the foreseeable future . -
pubmed
All forms of
cyt c6 were functionally characterized by laser flash absorption spectroscopy , and their stability was probed by
urea - induced folding equilibrium relaxation experiments and differential scanning calorimetry . -
pubmed
Replacement of D72 by
asparagine or
lysine increased the efficiency of electron transfer to
photosystem I , but destabilized the protein . -
pubmed
pulses of
methylprednisolone was observed , followed by a significant increase in
BALP after completing the oral
prednisone therapy . -
pubmed
During
steroid treatment of TO ( group I ) no dynamic changes of
DPD levels were observed . -
pubmed
Several features of
MYPT2 and
MYPT1 were similar , including : a specific interaction with the
catalytic subunit of type 1 phosphatase , delta isoform (
PP1cdelta ) ; interaction of
MYPT2 with the small heart - specific MP subunit ; interaction of the C - terminal region of
MYPT2 with the active form of
RhoA ; phosphorylation by
Rho - kinase at an inhibitory site ,
Thr646 and thiophosphorylation at
Thr646 inhibited activity of the
MYPT2 -
PP1cdelta complex . -
pubmed
Overexpression of
MYPT2 and
PP1cdelta blocked the
angiotensin II - induced sarcomere organization in cultured cardiomyocytes . -
pubmed
Acetate is transported into the brain through
MCA transporters that are also used for
lactate and
ketones . -
pubmed
This upregulation would potentially allow a similar twofold increase in the transport of other MCAs , including
lactate , during
insulin - induced
hypoglycemia . -
pubmed
The
enzyme can be inhibited by oxidant (
H ( 2 ) O ( 2 ) ) , solvent (
Tetrahydrofuran ) and several metal ions including
Hg ( 2 + ) ,
Fe ( 2 + ) and
Co ( 2 + ) . -
pubmed
This ( R)-stereospecific
epoxide hydrolase exhibits high enantioselectivity ( enantiomeric excess value , 99 % ) for the less hindered
carbon atom of
epoxide . -
pubmed
Correspondingly , a reduced capacity for up regulation of
CD62P expression and down regulation of
CD42b was observed in PCT PLTs after stimulation by the agonists
ADP or
TRAP . -
pubmed
Tsk ( 33.4+/-0.6 degrees C vs. 27.7+/-0.7 degrees C ) and
Tre ( 37.4+/-0.6 degrees C vs. 37.0+/-0.6 degrees C ) were significantly higher in H. Tm was 38.7+/-1.1 degrees C in H.
GE was lower in heat . -
pubmed
Cocaine positive hairs and seized
cocaine samples ( diluted to relevant concentrations ) were equally preprocessed and analyzed by LC -
MS -
MS . -
pubmed
Thus ,
HIPK2 could induce
beta - catenin protein degradation that was prevented by cell treatment with proteasome inhibitor
MG132 . -
pubmed
The
FaCCD1 protein cleaves
zeaxanthin ,
lutein , and
beta - apo-8 '- carotenal in vitro . -
pubmed
In the past decade , there are more than 2 000 published papers studying the clinical application , pharmacodynamic mechanism and structure - activity relationship (
SAR ) of
berberine and its derivatives , for treating
tumor ,
diabetes ,
cardiavascellum disease ,
hyperlipemia ,
inflammation ,
bacterium and virus infection ,
cerebral ischemia trauma ,
mental disease ,
Alzheimer disease ,
osteoporosis , and so on . -
pubmed
This paper is a systematic review of the research advances of
berberine and its derevatives in clinical application , pharmacodynamic mechanisms , molecular pharmacology , absorption and metabolism , and
SAR studies . -
pubmed
It has been reported that oral premedication with the
H ( 2 ) receptor antagonist
famotidine augmented intraoperative
hypothermia . -
pubmed
We again investigated whether the
H ( 2 ) receptor antagonist
famotidine significantly affected body temperature during open abdominal surgery under general anesthesia . -
pubmed
In
Digestive Disease Week 2008 , interesting advances in the treatment of
hepatitis C were presented , especially related to the promising increase in the therapeutic efficacy of the combination of conventional treatment with the
protease inhibitor ,
telaprevir . -
pubmed
The cell cycle checkpoint status (
p21 ,
p27 ,
p16 ,
Tp53 ) , cell dynamics (
proliferating cell nuclear antigen ,
Ki-67 ,
terminal deoxynucleotidyl transferase - mediated
dUTP -
biotin nick - end labeling , M30 ) , DNA damage (
gamma - H2AX [
H2A histone family , member X ] ) , telomere lengths ,
chromosomal instability ( micronuclei index ) , and senescence - associated
beta - galactosidase (
SA - beta - Gal ) activity were evaluated using an in situ approach and compared to those in normal liver ( n = 5 ) and liver with
chronic cholestasis ( 34 cases of
hepatolithiasis and three cases of
primary biliary cirrhosis ) . -
pubmed
Using 15 filter paper units of
cellulase per gram cellulose , a digestibility of 95 % was achieved by the two - stage method , which was higher than 81 % obtained from a 1 %
H ( 2 ) SO ( 4 ) pretreatment ( 200 degrees C , 8 min ) . -
pubmed
The generation of reactive
oxygen species ( ROS ) by the
nicotinamide adenine dinucleotide phosphate oxidase is an important mechanism by which neutrophils kill pathogens . -
pubmed
These components form an active complex at the correct time and subcellular location through a series of incompletely understood mutual interactions , regulated , in part , by
GTP /
GDP exchange on
Rac , protein phosphorylation , and binding to lipid messengers . -
pubmed
However , when the side chain of the midspan
Arg ( 694 ) " snorkels " to the outer leaflet of the viral membrane , the
MSD assumes a metastable conformation where the highly - conserved N - terminal core ( between
Lys(681 ) and
Arg(694 ) and containing the GXXXG motif ) unfolds . -
pubmed
In contrast , when the
Arg(694 ) side chain snorkels to the inner leaflet , the
MSD peptide assumes a metastable conformation consistent with experimental observations where the peptide kinks at
Phe(697 ) to facilitate
Arg(694 ) snorkeling . -
pubmed
RESULTS :
SiP could reduce apoptosis rate ( P & lt ; 0.01 ) and stabilize the mitochondrial membrane potential ( P & lt ; 0.05 ) and improved the level of p -
Akt1 ( P & lt ; 0.01 ) in
hypoxia / reoxygenation cardiomyocytes significantly . -
pubmed
Stabilization of mitochondrial membrane potential by
P13K - AM signaling pathway is likely to play a role in protective action of
S1P. -
pubmed
Peroxidase activity was insensitive to oxidizing agents such as
trans-4,4 - diydroxy-1,2 - dithiane or
hydrogen peroxide , but was inhibited by treatment of thylakoids with reducing agents such as
dithiothreitol or
mercaptoethanol . -
pubmed
We found that
methylprednisolone not only significantly decreased serum
TGF - beta1 ,
CTGF and
troponin T , but also reduced myocardial expressions of
TGF - beta1 ,
CTGF and
Smad3 after CME . -
pubmed
Stress treatments included sandblasting , leaf cutting and spraying with
jasmonic acid ,
salicylic acid , chitosan
oligosaccharide ( COS ) ,
H ( 2 ) O ( 2 ) (
HP ) and
NaCl ( SC)at different concentrations . -
pubmed
N - Terminal PEGylation of
hGH was carried out by covalent attachment of
PEG to the
alpha - amine residue of
Phe1 that yielded the monoconjugate
PEG - Nter -
hGH with a mass of 44152.2 Da , as measured by MALDI -
TOF mass spectrometry . -
pubmed
The
mTGase mediated PEGylation , performed in a water /
ethanol solution mixture , allowed a
PEG coupling reaction only at the level of hGH
Gln141 , yielding the single monoconjugate
PEG - Gln141 - hGH with a mass of 44064.9 Da . -
pubmed
Recognizing the potential pitfalls of
hydrogen isotopic analysis of
keratins with steam equilibration to infer origins of wildlife , food , and people . -
pubmed
Analyses examined fractional anisotropy (
FA ) and mean diffusivity ( MD ) between adolescents with low and high levels of problematic
alcohol use as assessed by the
Alcohol Use Disorders Identification Test ( AUDIT ) . -
pubmed
First ,
carboxylic acid - functionalized alkali lignin ( AL -
COOH ) was prepared and subsequently incorporated into
anhydride - cured
epoxy networks via a one -
pot method . -
pubmed
MTT , colony formation assay and matrigel invasion assay were carried out to assess the role of
cytoglobin on cell proliferation and invasion . -
pubmed
After adjustment for potential confounders , when compared to
trastuzumab patients ,
lapatinib patients had a higher rate of treatment discontinuation ( hazard ratio [ HR ] = 1.57 ; P & lt ; 0.001 ) , a higher rate of outpatient visits ( not treatment administration related ) (
IRR = 1.19 ; P & lt ; 0.004 ) , and a lower rate of medical visits associated with treatment administration ( IRR = 0.34 ; P & lt ; 0.001 ) . -
pubmed
lactis CRL 581 possesses only one gene that encodes
PrtL , which consists of 1924
amino acids and is a multidomain protein anchored to the cell via its W domain . -
pubmed
PrtL was extracted from the cell under high ionic strength conditions using
NaCl , suggesting an electrostatic interaction between the
proteinase and the cell envelope . -
pubmed
Under optimal conditions ,
PrtL exhibited higher affinity for
succinyl - alanyl - alanyl - prolyl - phenylalanine - p - nitroanilide than for
succinyl - alanyl - glutamyl - prolyl - phenylalanine - p - nitroanilide , while
methoxy - succinyl - arginyl - prolyl - tyrosyl - p - nitroanilide was not degraded . -
pubmed
Finally , on the basis of its specificity towards
caseins and the unique combination of
amino acids at residues thought to be involved in substrate specificity ,
PrtL can be classified as a representative of a new group of
CEP . -
pubmed
Anti -
ERp5 antibody inhibited
ERp5 - dependent platelet and endothelial cell
disulfide reductase activity in vitro . -
pubmed
ERp5 release at the
thrombus site was detected after infusion of
Alexa Fluor 488 - labeled anti -
ERp5 antibody at 0.05 mug / g body weight , a dose that does not inhibit
thrombus formation . -
pubmed
The
cysteine residues in the
ERp5 active sites are not required for binding to
beta3 integrin . -
pubmed
Among therapy drugs that lower
urate ,
benzbromarone (
BBR ) , an inhibitor of
urate transporters , is widely used because it is well tolerated and highly effective . -
pubmed
We demonstrate that
BBR is also an activator of
voltage - gated KCNQ potassium channels . -
pubmed
In cultured recombinant cells ,
BBR exhibited significant potentiation effects on
KCNQ channels comparable to previously reported classical activators . -
pubmed
In native dorsal root
ganglion neurons ,
BBR effectively overcame the suppression of
KCNQ currents , and the resultant neuronal hyperexcitability caused by inflammatory mediators , such as
bradykinin ( BK ) . -
pubmed
Notably , the analgesic effects of
BBR are largely mediated through peripheral and not through central
KCNQ channels , an observation supported both by pharmacokinetic studies and in vivo experiments . -
pubmed
Moreover , multiple residues in the superficial part of the voltage sensing domain of
KCNQ channels were identified critical for the potentiation activity of
BBR by a molecular determinant investigation . -
pubmed
Our data indicate that activation of peripheral
KCNQ channels mediates the
pain relief effects of
BBR , potentially providing a new strategy for the development of more effective therapies for
gout . -
pubmed
In group 3 , tail blood samples were collected from animals before ( T0 ) and 24 hours after (
T1 )
cyclophosphamide (
CP ) delivery . -
pubmed
Hexavalent chromium - induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of
alpha - tubulin . -
pubmed
Cerdulatinib , a novel dual
SYK /
JAK kinase inhibitor , has broad anti -
tumor activity in both ABC and
GCB types of
diffuse large B cell lymphoma . -
pubmed
Herein , we investigated the
anti - lymphoma activity of
cerdulatinib , a novel compound that dually targets
SYK and
JAK /
STAT pathways . -
pubmed
Importantly ,
JAK /
STAT and
BCR signaling can be blocked by
cerdulatinib in primary
GCB and non - GCB DLBCL
tumor cells that were accompanied by cell
death . -
pubmed
Our work provides mechanistic insights into the actions of
cerdulatinib , suggesting that the drug has a broad anti -
tumor activity in both ABC and
GCB DLBCL , at least in part by inhibiting
SYK and
JAK pathways . -
pubmed
Impact of
CYP3A5 polymorphism on trough concentrations and outcomes of
tacrolimus minimization during the early period after kidney transplantation . -
pubmed
CONCLUSIONS : Although
CYP3A5 polymorphism significantly influenced the
tacrolimus dose required to achieve the target concentration , the impact of
CYP3A5 polymorphism on BPAR was not observed in this study . -
pubmed
Excess
betaLGm preferentially entered the
AMP phase at pH & gt ; 5.0 and the
XG phase at lower pH. -
pubmed
The inversion was caused by adsorption of
XG onto
betaLGm that started below pH 5.5 . -
pubmed
Moreover , the increase of
potassium currents by
cisplatin was further inhibited by the application of
TEA confirming the action of
cisplatin on
potassium channels . -
pubmed
Moreover , the increase of
potassium currents by
cisplatin was further inhibited by the application of
TEA confirming the action of
cisplatin on
potassium channels . -
pubmed
RESULTS :
Stretozotocin (
STZ ) only - treated groups exhibited
hyperglycemia ,
polydipsia ,
polyuria ,
weight loss ,
glucose intolerance , low fasting plasma
insulin and reduced hepatic glycogen content compared to the control group . -
pubmed
Naringin treatment significantly improved Fasting Plasma
Insulin ( FPI ) , hepatic glycogen content ,
malondialdehyde ,
beta - hydroxybutyrate ,
acetoacetate ,
bicarbonate , blood pH and anion gap but not Fasting Blood
Glucose ( FBG ) compared to the
STZ only - treated group . -
pubmed
, ECG and respiratory motion gating )
( 1 ) H -
MRS was used to quantify myocardial
triglyceride levels for each gender . -
pubmed
CONCLUSIONS : The study showed that
( 1 ) H -
MRS is a capable , noninvasive tool for acquisition of myocardial
triglyceride metabolites . -
pubmed
Investigation of binding properties of dicationic
styrylimidazo [ 1,2 - a ] pyridinium dyes to
human serum albumin by spectroscopic techniques . -
pubmed
The binding interaction between two dicationic
styrylimidazo [ 1,2 - a ] pyridinium dyes and
human serum albumin (
HSA ) was investigated at physiological conditions using fluorescence , UV - vis absorption , and circular dichroism ( CD ) spectroscopies . -
pubmed
The calculated thermodynamic parameters ( DeltaG , DeltaH and DeltaS ) indicated that
hydrogen bonding and van der Waals forces played a major role in the formation of the dye -
HSA complex . -
pubmed
AIM : To comparatively evaluate the efficacy of
magnesium sulphate as an adjunct to
ropivacaine in local infiltration for
postoperative pain following lower segment cesarean section . -
pubmed
First tested these five concentrations of
PCA2 ( 3 - 300 muM ) alone and in combination with
BPA(100 mug / L ) , on
insulin secretion from isolated islets at in vitro condition . -
pubmed
At in vivo condition , the
BPA ( 100 mug / kg ) and
PCA2 ( 10 mumol / kg ) administered for 20 days then , blood
glucose and
insulin ,
Pdx1 and ,
Glut2 genes expression , and oxidative stress markers examined . -
pubmed
The results indicated that
PCA2 strongly prevents islet cells apoptosis induced by
BPA and , co - administration of
PCA2 and
BPA modified
hyperglycemia . -
pubmed
BPA reduced
Pdx1 and
Glut2 mRNA expression and antioxidant level in pancreas tissue , whereas
PCA2 prevented from these effects . -
pubmed
The labelling efficiency with three stable specific immunoplasmonics labels ( functionalized
silver nanospheres ( CD44 - AgNSs ) , gold (
Au ) NSs (
EGFR - AuNSs ) and
Au nanorods (
KV1.1 - AuNRs ) ) detected by reflected light microscopy ( RLM ) is similar to the one with immunofluorescence . -
pubmed
We genotyped two selected polymorphisms of the
tryptophan hydroxylase 1 (
TPH1 ) gene ( rs1800532 218A>C and rs1799913 779A>C ) and polymorphism in the promoter region of
serotonin transporter gene ( 5 - HTTLPR , rs25531 ) related to serotoninergic neurotransmission . -
pubmed
This situation triggers a transcriptional response , mediated by the
hypoxia inducible factors (
HIFs ) , that aims to restore
oxygen homeostasis . -
pubmed
RORalpha is a member of the
nuclear receptor super family and plays important roles in lipid ,
glucose metabolism , as well as the inflammatory response . -
pubmed
Conversely , selective antagonism (
SR1001 ) abrogated the induction of
M2 macrophage markers which induced by CH - S. In addition , CH - S induced phosphorylation of
Adenosine monophosphate ( AMP )- activated protein kinase alpha (
AMPKalpha ) , which was accompanied by the activation of
acetyl - CoA carboxylase 1 (
ACC ) . -
pubmed
However ,
SR1001 abolished the activation of
AMPKalpha and
ACC induced by CH - S. Meanwhile , inactivation of
AMPKalpha by its inhibitor
Compound C ( CompC ) abrogated the mRNA and protein levels of CH - S - induced M2 macrophage markers expressions . -
pubmed
The non - receptor
tyrosine phosphatase
Ptpn11 (
Shp2 ) is an important transducer of growth factor and cytokine signals . -
pubmed
PI3Ks have dual
kinase specificity : a
lipid kinase activity that phosphorylates the 3'-
hydroxyl of
phosphoinositides and a
protein - kinase activity that includes autophosphorylation . -
pubmed
Our results revealed that
Lys-776 , located in the P - loop of
PI3Kalpha , is essential for the recognition of lipid and
ATP substrates and also plays an important role in
PI3Kalpha autophosphorylation . -
pubmed
Replacement of the residues
His-936 and
His-917 in the activation and catalytic loops , respectively , with
alanine dramatically changed
PI3Kalpha kinetics . -
pubmed
Compared to the CM model group , the CM +
AmB and CM +
PD-1 Ab groups exhibited increased levels of Th1
cytokines and
chemokines expression , and reduced levels of Th2
cytokines expressions . -
pubmed
This study demonstrates that changes in circulating
insulin that are too fine to adversely affect
glucose homeostasis nonetheless exert control over adiposity.-Page , M. M. , Skovso , S. , Cen , H. , Chiu , A. P. , Dionne , D. A. , Hutchinson , D. F. , Lim , G. E. , Szabat , M. , Flibotte , S. , Sinha , S. , Nislow , C. , Rodrigues , B. , Johnson , J. D. Reducing
insulin via conditional partial gene ablation in adults reverses diet - induced
weight gain . -
pubmed
Moreover , the presence of
Snail or
Twist1 partially blocked
phorbol 12 - myristate 13 - acetate - induced megakaryocyte differentiation , while that of
Twist significantly altered
imatinib - induced erythroid differentiation . -
pubmed
Lastly , coronal cells express the
Trpa gene , which encodes an
ion channel expressed in hair cells . -
pubmed
Furthermore ,
BETi reduce growth of
prostate cancer cells and patient - derived organoids with known
AR mutations ,
AR amplification and
AR -
V7 expression . -
pubmed
Conclusions :
BETi merit clinical evaluation as inhibitors of
AR splicing and function , with trials demonstrating their blockade in proof - of - mechanism pharmacodynamic studies . -
pubmed
Hcp / fcc nucleation in bcc
iron under different anisotropic compressions at high
strain rate : Molecular dynamics study . -
pubmed
In this study , a milk protein - derivative is shown to increase
GHSR-1a - mediated intracellular
calcium signalling in a concentration - dependent manner in vitro . -
pubmed
Herein , we successfully synthesized partially oxidized 5 nm
cobalt nanoparticles dispersed on a single - layer
nitrogen - doped
graphene ( SL - NG ) ( denoted as
PO-5 nm
Co / SL - NG ) catalyst by a unique and simple one -
pot synthesis strategy , which was efficiently applied for highly selective electrocatalytic reduction of
carbon dioxide to
methanol in 0.1 mol dm-3 aqueous
NaHCO3 medium under mild conditions , reaching the maximum faradaic efficiency (
FE ) of 71.4 % for
methanol at -0.90 V versus saturated
calomel electrode ( SCE ) , possessing a strong electrocatalytic current density of 4 mA cm-2 and a high yield of 1.10 mmol dm-3 h-1 , and the corresponding overpotential is as low as 280 mV. -
pubmed
Moreover , at -1.0 V versus SCE , a high current density of 10 mA cm-2 can be obtained , and the
FE for
methanol still remains 23.2 % . -
pubmed
This work first provides an advanced
PO-5 nm
Co / SL - NG for selective electroreduction of
carbon dioxide into
methanol , which simultaneously possesses the merits of high current density , low overpotential , high selectivity , superior
FE , and good stability , outperforming most reported electrocatalysts . -
pubmed
Determination of adsorbable organically bound
fluorine ( AOF ) and adsorbable organically bound
halogens as sum parameters in aqueous environmental samples using combustion ion chromatography (
CIC ) . -
pubmed
Because of their toxicity and biomagnification potential individual
perfluoroalkyl and
polyfluoroalkyl substances (
PFAS ) are regularly examined in food and environmental matrices by LC -
MS /
MS . -
pubmed
The combustion ion chromatography (
CIC ) can be used to determine adsorbable organic
fluorine ( AOF ) in aqueous samples . -
pubmed
CIC analysis was performed in 22 samples from a chemical company wastewater treatment plant , and 14 individual
PFAS were determined by LC -
MS /
MS . -
pubmed
AOF values up to 555 mug / L were found in these samples while the total of the individual
PFAS , calculated as
fluorine , was 8.8 mug / L. This provides evidence , that
CIC covers a huge range of fluoroorganic compounds that are presently not detected by LC -
MS /
MS . -
pubmed
Furthermore , the
CIC method allowed the determination of four
halogens in 26 surface water samples . -
pubmed
This demonstrated that the
CIC technique can be used as a powerful screening test to support LC -
MS /
MS methods , and is also useful to detect organic
chlorine ,
bromine and
iodine compounds ( AOCl ,
AOBr and
AOI ) . -
pubmed
The aim of this study was to investigate the distribution and retention of different chemotypes typifying available inhaled drugs [ slowly dissolving neutral
fluticasone propionate (
FP ) and soluble bases
salmeterol and
salbutamol ] using mass spectrometry imaging ( MSI ) . -
pubmed
In the same animals ,
salmeterol - d3 ,
salbutamol - d3 , and
FP - d3 were delivered by intravenous (
IV ) injection . -
pubmed
At the 30 - minute time point , inhaled
salmeterol and
salbutamol were preferentially retained in bronchiolar tissue , whereas
FP was retained in all regions of the lungs . -
pubmed
5 ' RACE experimental data showed that
bna - miR827a cleaves three
NITROGEN LIMITATION ADAPTATION (
NLA ) genes (
BnaC08g45940D ,
BnaA10g01450D and
BnaC05g01480D ) . -
pubmed
The expression levels of a
phosphate ( Pi)-related miRNA ,
bna - miR827a , and its target genes were affected in
wax - deficient rapeseeds . -
pubmed
Among all of the
nitroaromatics , the highest fluorescence quenching is observed for
nitrophenols ( say
picric acid (
PA ) ) . -
pubmed
Perrin 's model of quenching sphere action has been attributed to
nitrophenol (
PA ) selective signaling behavior of
biaryl systems . -
pubmed
Synergistic interactions were observed when
DosRST inhibitors were examined in pairwise combinations with the strongest potentiation observed between
artemisinin paired with HC102A , HC103A , or HC106A. -
pubmed
GRP94 is an
ATP - dependent chaperone able to regulate pro - oncogenic signaling pathways . -
pubmed
In this work , an untargeted lipidomic analysis revealed that some lipid species were altered in
GRP94 - deficient cells , specially
GM2 and
GM3 gangliosides . -
pubmed
The catalytic pathway of GM2 is affected by the low enzymatic activity of
beta - Hexosaminidase (
HexA ) , responsible for the hydrolysis of
GM2 to
GM3 . -
pubmed
In this work , based on the strategy of label - assisted chemical adsorption triggered conversion of electroactivity of sensing interface , a
Ag /
AgCl process was achieved to enhance sensitivity of the constructed sandwich - type amperometric immunosensor for ultrasensitive detection of
carbohydrate antigen 19 - 9 (
CA19 - 9 ) . -
pubmed
The '
Coal Replacing Project ' (
CRP ) , replacing coal with cleaner energy like natural gas and electricity , was implemented in North China to curb PM2.5 pollution ; therefore , it is important to explore the sources and transformation mechanisms of PM2.5
nitrate under this strategy for examining its effectiveness . -
pubmed
The results showed that the average concentrations of PM2.5 and its dominant inorganic ions (
SO42 - ,
NO3 - ,
NH4 + ) in C3 during the
CRP , compared to
C2 , decreased by 62.13 % , 79.69 % , 55.14 % and 38.84 % , respectively , attesting the improvement of air quality during the
CRP . -
pubmed
According to the correlation between
[ NO3 -/ SO42 - ] and [
NH4 +/ SO42 - ] as well as
delta18O variations , the homogeneous formation pathway might be dominant in
C1 , while the heterogeneous pathway would be primary in
C2 and
C3 during the formation of
nitrate . -
pubmed
The dominant sources in
C1 were biogenic soil emission ( 37.0 % + - 9.9 % ) and mobile emission ( 25.7 % + - 19.1 % ) , while
coal combustion ( 42.4 % + - 13.8 % in
C2 and 34.9 % + - 14.4 % in
C3 ) and biomass burning ( 31.0 % + - 21.2 % and 34.7 % + - 22.7 % ) were the main sources in
C2 and
C3 . -
pubmed
In the winter , the contribution of
coal combustion dropped by about 8 % during the
CRP (
C3 ) in comparison with that in
C2 , suggesting the implementation of
CRP played an important role in reducing NOX from
coal combustion . -
pubmed
Further studies revealed that
MS prevented CSDS - evoked augmentation of glutamatergic transmission onto principal neurons ( PNs ) in the basolateral amygdala ( BLA ) by inhibiting presynaptic
glutamate release . -
pubmed
Diclofenac sodium - loaded
sodium alginate /
hydroxyapatite /
halloysite nanotubes (
SA / HA / HNTs -
DS ) nanocomposite hydrogel beads were prepared by the in situ generation of HA nanoparticles during the sol - gel transition of the
SA / HNTs suspension . -
pubmed
, the weight ratio of HNTs to
SA ( mHNTs / mSA ) , the weight ratio of
( NH4 ) 2HPO4 to
SA ( m ( NH4 ) 2HPO4 /mSA ) , and the concentration of
SA , which influenced the entrapment efficiency ( EE ) and release of
DS , were investigated . -
pubmed
An almost constant rate release of
DS is achieved when mHNTs / mSA = 0.3 and m
( NH4 ) 2HPO4 /mSA = 0.1 . -
pubmed
Simeprevir ,
daclatasvir , and
sofosbuvir for
hepatitis C virus - infected patients : Long - term follow - up results from the open - label , Phase
II IMPACT study . -
pubmed
BACKGROUND :
Hyaluronic acid ( HA ) is a key contributor to skin moisture ( hydration ) , and Microbotox demonstrates improvements for fine wrinkles of the
face . -
pubmed
Using the
hydrotoxin mixture , two ingredients could be injected on the exact dermal layer of
faces in a session . -
pubmed
This inhibitory effect of
VEGF was not influenced by masking of
phosphatidylserine (
PS ) on the surface of apoptotic cells and was partially mediated by the
PI3K -
Akt signaling pathway . -
pubmed
After the microcosms were sparged with
hydrogen gas , samples were taken regularly within 122 h for
nitrate and
nitrite measurements , community composition analysis via
16S rRNA gene amplicon sequencing , and gene and transcript quantification via qPCR of
reductase genes essential for complete denitrification . -
pubmed
In this study , a multicolor sensing system has been established for simultaneous determination of four foodborne bacteria by exploiting
oxidase mimicking activity of aptamer - functionalized
manganese dioxide - coated
ferriferrous oxide ( apt -
Fe3O4 /
MnO2 ) nanocomposites and oxTMB etching of gold nanorods ( AuNRs ) . -
pubmed
Effect of various inhibitors of
factor XIII on the patterns in
SDS - electrophoresis and crossed immunoelectrophoresis . -
pubmed
The effect of different
factor XIII inhibitors ( competetive inhibition , interference with active center
SH - groups in different ways ,
Ca2 + depletion ) on the sequence of the gamma - dimerization and alpha - polymerization of
fibrin is examined by
sodium dodecyl sulphate polyacrylamide gel electrophoresis (
SDS - PAE ) ( reduced material ) . -
pubmed
Non - crosslinked
fibrin and
fibrin clots of different degrees of crosslinking are digested with
plasmin and the lysate tested in
SDS - PAE ( non - reduced material ) and crossed
agarose gel immunoelectrophoresis ( CAIE ) . -
pubmed
Minimally destructive methods were sought to assess the
factor VIII content of fresh frozen plasma intended for large - scale fractionation and stored in five - liter
polyethylene packs after pooling approximately 23 plasma donations . -
pubmed
While not directly inhibiting the growth of subcutaneous fibroblasts in vitro ,
dexamethasone does suppress the production and/or activity of
fibroblast growth factors generated through macrophage - EL4 cell interactions in vitro . -
pubmed
We have examined the effects of fetal
hypoxaemia , produced by reducing the percent
oxygen in maternal inspired air , on fetal plasma concentrations of
corticotrophin releasing hormone (
CRH ) , adrenocorticotrophin (
ACTH ) and
cortisol and determined the effects of an
opioid receptor antagonist ,
naloxone on these responses . -
pubmed
Hypoxaemia ( fetal PO2 , 15 - 18 mmHg ) for 60 min provoked a significant ( P & lt ; 0.05 ) increase in fetal plasma
ACTH and
cortisol concentrations at days 125 - 130 of pregnancy , but did not affect circulating
CRH . -
pubmed
There was no effect of
naloxone administered either intravenously ( 1.25 mg bolus followed by a 2.5 mg / h continuous infusion for one hour ;
fetal body weight approximately 2.5 Kg ) or via the lateral cerebral ventricle ( 50 micrograms bolus followed by a 100 micrograms / h infusion for one hour ) on this pattern of
ACTH and
cortisol change nor on the lack of
CRH response to
hypoxaemia . -
pubmed
We conclude that the increase in fetal ACTH and
cortisol in response to acute
hypoxaemia is not accompanied by an increase in systemic
CRH concentrations , nor is the response dependent on short - term opioid regulation . -
pubmed
Induction of this enzyme was accompanied by a decrease in the constitutive
NO synthase activity , a phenomenon which was prevented or reversed by
dexamethasone ( 1 microM ) . -
pubmed
Cytochalasans and
PMA induce
IL-2 receptors on
CD8 + lymphocytes . -
pubmed
The
phorbol ester tumor promoter ,
PMA , alone is not mitogenic for primary lymphocytes but enhances the activity of
mitogenic lectins . -
pubmed
Because the
cytochalasans have been reported to increase intracellular
Ca2 + and because
PMA activates
protein kinase C , lymphocytes were treated with
PMA and
cytochalasin B (
CyB ) to determine if this combination would induce DNA synthesis . -
pubmed
While this treatment by itself did not cause proliferation , lymphocytes cultured with
PMA and
CyB overnight , washed , and recultured with
IL-2 proliferated to the same degree as lymphocytes stimulated with Con A. -
pubmed
Three different
cytochalasans ,
cytochalasin B ,
cytochalasin D , and
chaetoglobosin C , all of which bind to cellular
actin with different affinities and only one of which affects
glucose transport , induced
IL-2 receptors in combination with
PMA . -
pubmed
However , no
IL-2 was produced after the exposure of lymphocytes to the combination of
cytochalasans and
PMA . -
pubmed
We recently reported that autophosphorylated
protein kinase C (
PKC ) has an intrinsic
Ca ( 2 +)- and phospholipid - dependent
ATPase activity and that the
ATPase and
histone kinase activities of
PKC have similar metal - ion cofactor requirements and Km , app(
ATP ) values . -
pubmed
These observations support a mechanism of
PKC catalysis in which the productive binding of phosphoacceptor substrates enhances the rate of
phosphodonor substrate (
ATP ) hydrolysis at the active site of
PKC . -
pubmed
In fact , sequence analysis indicates that
PKC contains a potential second
nucleotide binding site outside of its active site . -
pubmed
We show that the regulatory and catalytic properties of the
ATPase reactions of three
PKC isozymes are similar , despite critical differences among the isozymes in their consensus sequences for the potential non - active - site
nucleotide binding site in their catalytic domains . -
pubmed
We also show that the
ATPase and
histone kinase reactions of each isozyme have similar Km , app(
ATP ) values. -
pubmed
Two and five days after
FAS injections into the amygdala , the density of
muscarinic receptor decreased about 50 % as measured by the specific bindings of
3H - quinuclidinyl benzilate and
3H - oxotremorine . -
pubmed
The production of
inositol bisphosphate and the release of
Ca2 + from intracellular stores induced by
IgE - antigen stimulation were enhanced in
3C7 . -
pubmed
Hypolipidemic effect of
polymethylenemethane thiosulfonates : inhibitors of
acetoacetyl coenzyme A thiolase . -
pubmed
Their action was based on their specific inhibitory effect on cytoplasmic
acetoacetyl - CoA thiolase , one of the key enzymes in
cholesterol biosynthesis . -
pubmed
In the presence of
Mg2 + alone , the
leuX precursor was found to be self - cleaved at a site approximately 13 nt inside from the 5 ' end of mature
tRNA . -
pubmed
The basic comparison was made from the staining patterns for the myofibrillar
actomyosin ATPase produced by preincubation of fresh frozen cross - sections of muscle at alkaline pH ( 10.30 ) or acid pH ( 4.60 ) with those produced by preincubation in media containing
Cu2 + at alkaline pH ( 10.30 ) , near neutral pH ( 7.40 ) , or acid pH ( 4.60 ) . -
pubmed
Type
II fibers in mixed fibered muscles were either very sensitive , moderately sensitive , or relatively insensitive to inactivation of the
myofibrillar actomyosin ATPase after
acid preincubation . -
pubmed
The single
prostacyclin receptor of gel - filtered platelets provides a correlation with antiaggregatory potency of
PGI2 mimics . -
pubmed
Platelet - rich plasma (
PRP ) displays the same receptor density but the KD value increases to 123 nM due to protein binding of
PGI2 which lowers its effective concentration . -
pubmed
Antiaggregatory IC50s and radioligand binding IC50s correlate for
PGE2 ,
E1 , and six
PGI2 analogs . -
pubmed
In group I ,
atropine increased
sinus rate from 53.7 + /- 4 to 87.9 + /- 17 bpm ( delta % : 65.5 + /- 33 ) and in group
II from 51.6 + /- 5 to 73.9 + /- 14 bpm ( delta % : 43.1 + /- 26 ) . -
pubmed
The adherence was decreased by platelets and the active constituent was identified as
adenosine diphosphate which decreased the sticking of
fibrin to cellular linings as well as to artificial surfaces . -
pubmed
Ribonucleotide reductase is responsible for the production of the
deoxyribonucleotides required for DNA synthesis . -
pubmed
Bt2cAMP ) results in the
protein kinase - dependent arrest of the cells in the
G1 phase of the cell cycle . -
pubmed
Removal of
bt2cAMP after 24 h exposure resulted in increased holoenzyme and
protein M2 activities . -
pubmed
These results demonstrate that the
ribonucleotide reduction necessary for DNA synthesis is regulated in a cell cycle - dependent fashion by the activity of the
protein M2 subunit of ribonucleotide reductase . -
pubmed
A
PifC -
LacZ fusion protein of 149,000 daltons has been identified by immunoprecipitation and
polyacrylamide gel electrophoresis . -
pubmed
The maximum hemagglutination titer ( 1:64 to 1:256 ) was obtained against
GA - fixed RBC with the
KSCN - treated organisms that were also sonicated . -
pubmed
The activity was inactivated by heating at 100 C or by treatments with
formalin or
trypsin , but not by treatments with
hyaluronidase or
neuraminidase . -
pubmed
Evidence for a
carboxyl group in the vicinity of the
retinal chromophore of
bacteriorhodopsin . -
pubmed
Carboxyl groups of
bacteriorhodopsin in purple membranes were activated using a hydrophobic reagent and then covalently labeled with a pH - sensitive reporter group ,
nitrotyrosine methyl ester . -
pubmed
These results indicate that a
carboxyl group is near the
retinal chromophore of
bacteriorhodopsin . -
pubmed
Mutually facilitated binding of
cholesterol and
adrenodoxin . -
pubmed
The addition of
PCC also resulted in the modest expression of
B7 - 1 and a more robust expression of
B7 - 2 on the cognate B cells . -
pubmed
The
S6 monoclonal antibody to
somatostatin , but not control antibodies , enhanced
gastrin - stimulated
histamine release . -
pubmed
In the presence of
S6 , the
somatostatin analogue
SMS-201 - 995 ( 10(-7 ) M ) inhibited
gastrin - stimulated
histamine release by 95 % . -
pubmed
In contrast to
somatostatin ,
epinephrine failed to inhibit this
gastrin stimulation . -
pubmed
Furthermore , enhancement of
gastrin - stimulated
histamine release by immunoneutralization of
somatostatin indicates an important role for endogenous
somatostatin as a paracrine inhibitor of non - mast cell
histamine release . -
pubmed
MAIN OUTCOME MEASURES : We measured blood pressure , serum
creatinine , hematocrit , and
hemoglobin . -
pubmed
RESULTS : Bone lead levels in the patella were found to be significantly correlated with a decrease in
hemoglobin and hematocrit , even after adjusting for age , blood lead ,
body mass index , cigarette smoking , and
alcohol ingestion and removing outliers . -
pubmed
Several other agents including
pyrazole ,
4 - methylpyrazole ,
isoniazid ,
pyridine , and
DMSO also increased the content of
P4502E1 in this cell line but not oxidation of substrates , presumably a reflection of remaining tightly bound to the active site of
P4502E1 . -
pubmed
Slot blot analysis indicated that
ethanol addition did not increase
P4502E1 mRNA levels . -
pubmed
These results indicate that
ethanol can increase the content of
P4502E1 as well as catalytic oxidation of substrates dependent on
P4502E1 in this experimental model , perhaps by stabilization of the protein against degradation . -
pubmed
The different samples were homogenized and centrifuged ( 2000 g during 20 minutes ) , and the supernatant was incubated with
dog kininogen and 0.1 mol / L
Tris -
HCl buffer ( pH 8.5 ) in the presence of
peptidase inhibitors . -
pubmed
Tin - protoporphyrin ( 10 mumol / L ) produced an eightfold to 10 - fold increase in
erythropoietin RNA within 40 min . -
pubmed
Exposure to
cobalt chloride ( 100 mumol / L ) resulted in a 20 - fold to 30 - fold increase in
erythropoietin expression for 5 to 20 min , returning to control by 40 min . -
pubmed
Additionally , nuclear runoff assays demonstrated that the increase in
heme oxygenase or
erythropoietin messenger RNA accumulation by
cobalt chloride appeared to be a result of stimulated transcription of the
heme oxygenase and
erythropoietin genes . -
pubmed
Heme produced an immediate expression of
heme oxygenase RNA ( 50 - fold within 5 min ) and a second sustained response during the next 24 hr . -
pubmed
Thus both
erythropoietin and
heme oxygenase genes appear to be expressed after treatment with
tin - protoporphyrin ,
heme or
cobalt chloride ; however , the time and patterns of expression are different. -
pubmed
In this report we present the construction and analysis of 15 mutant alleles of the
topoisomerase containing
amino acid substitutions in a highly conserved region . -
pubmed
In sum , these studies suggest that this region of the
topoisomerase (
amino acids 216 - 225 ) plays a proximal role in mediating DNA cleavage and the covalent interaction between the 3'-
phosphoryl of the nicked DNA and
tyrosine 274 of the
vaccinia topoisomerase I. -
pubmed
The number of macrophages and their response to
LH to produce
IL-1 were increased significantly ( P & lt ; 0.01 ) by
estradiol treatment but not by
progesterone or
testosterone treatment . -
pubmed
Our results show that the higher concentrations of
ethylenediaminetetraacetic acid (
EDTA ) and
bovine serum albumin (
BSA ) in CZB medium , in comparison with KSOM , protect against the toxic component in the oil . -
pubmed
Unlike coiled
coil motor proteins ,
mitofilin is resistant to
detergent extraction . -
pubmed
The presence of mitochondrial targeting and stop - transfer sequences , along with the accessibility of
mitofilin to limited proteolysis suggests that it resides predominantly in the intermembrane space , consistent with immuno -
electron micrographs which show
mitofilin mainly at the mitochondrial periphery . -
pubmed
The induced increase in cytosolic [
Ca2 +]i is blocked by
sugar hapten inhibitors of
galectin-3 . -
pubmed
The
galectin-3 - induced effect is insensitive to voltage - gated
Ca2 + channel antagonists such as
prenylamine ,
nifedipine and
diltiazem and to
pertussis toxin but is inhibited by
cholera toxin . -
pubmed
The results suggest that
galectin-3 released by accessory cells such as macrophages may bind in vivo to
T - cell activation antigens and also participate in
Ca2 + signalling . -
pubmed
The DNA
helicases acting in
nucleotide excision repair ,
XPD ,
CSB and
XPB , are not required for
PCNA - dependent repair of abasic sites . -
pubmed
DNA repair of abasic sites is accomplished in mammalian cells by two distinct base excision repair ( BER ) pathways : a single
nucleotide insertion pathway and a
proliferating cell nuclear antigen (
PCNA)-dependent pathway involving a resynthesis patch of 2 - 10 nucleotides 3 ' to the lesion . -
pubmed
The latter pathway shares some enzymatic components with the
nucleotide excision repair (
NER ) pathway acting on damage induced by ultraviolet light : both pathways are strictly dependent on
PCNA and several observations suggest that the polymerization and ligation phases may be carried out by common enzymatic activities (
DNA polymerase delta / epsilon and
DNA ligase I ) . -
pubmed
No defect of either the
PCNA - dependent or the single
nucleotide insertion pathways could be observed in UV5 , UV61 or 27 - 1 mutant cell extracts , thus showing that the partial enzymatic overlap between
PCNA - dependent BER and
NER does not extend to
DNA helicase activities . -
pubmed
Expression of
heme oxygenase-1 mediated by non -
NMDA and
metabotropic receptors in glial cells : possible involvement of reactive
oxygen species production and
protein kinase C activation . -
pubmed
Heme oxygenase (
HO ) produces
biliverdin and
bilirubin which are physiological antioxidants and potent scavengers of
oxygen radicals . -
pubmed
The
HO-1 protein level was significantly enhanced by several agonists for
non - N - methyl - D - aspartate ( non - NMDA ) receptors and
metabotropic glutamate receptors (
mGluR ) such as KA ,
quisqualic acid (
QA ) ,
( + /-)- alpha - amino-3 - hydroxy-5 - methylisoxazole-4 - propanoic acid (
AMPA ) , and
trans -(+/-)- 1 - amino -( 1S,3R )- cyclopentanedicarboxylic acid (
ACPD ) . -
pubmed
KA - induced
HO-1 expression was inhibited by the non -
NMDA antagonist
NBQX . -
pubmed
In addition , KA induced the marked production of reactive
oxygen species ( ROS ) , and KA - induced
HO-1 expression was also inhibited by the antioxidants
allopurinol and
ascorbic acid . -
pubmed
ACPD - induced
HO-1 expression was inhibited by the
mGluR antagonist
MCPG and the
protein kinase C (
PKC ) inhibitor
calphostin C. -
pubmed
Vesicular monoamine transporters (
VMATs ) are involved in chemical transduction in monoaminergic neurons and various endocrine cells through the storage of
monoamines in secretory vesicles . -
pubmed
The
5 - HT uptake was inhibited significantly by
bafilomycin A1 ( an inhibitor of
vacuolar H +- ATPase ) ,
3,5 - di - tert - butyl-4 - hydroxybenzylidenemalononitrile ( a proton conductor ) , or
reserpine ( an inhibitor of
VMAT ) . -
pubmed
The
5 - HT immunoreactivity in the long branching processes disappeared upon incubation with
reserpine . -
pubmed
Our results suggest that
PTH modulates the expression of
ClC-5 in the kidney cortex and that neither
1alpha,25 ( OH )( 2 ) vitamin D ( 3 ) nor
PTH regulates
ClC-5 expression in the medulla . -
pubmed
The pattern of expression of
ClC-5 varies with urinary
calcium . -
pubmed
Insulin increases renal
sodium reabsorption which may contribute to
hypertension . -
pubmed
The
meglitinides are nonsulfonylurea agents that are also
insulin secretagogues . -
pubmed
Unlike the
sulfonylureas ,
repaglinide appears to require the presence of
glucose to close the
adenosine triphosphate - sensitive potassium channels and induce
calcium influx . -
pubmed
Disaccharidase inhibitors effectively compensate for the defective early - phase
insulin release by slowing the production of
sugars from
carbohydrates . -
pubmed
Thiazolidinediones appear to activate
peroxisome proliferator - activated receptor gamma , which is involved in the metabolism of lipids . -
pubmed
The combined use of the early detection of
alcohol consumption ( EDAC ) test and
carbohydrate - deficient
transferrin to identify heavy drinking behaviour in males . -
pubmed
The aim of this study was to determine the efficacy of the combined use of
carbohydrate - deficient
transferrin (
CDT ) and the Early Detection of
Alcohol Consumption ( EDAC ) test to assess heavy drinking in a population of males ( n = 187 ) drinking an average of 20 drinks per day . -
pubmed
Siglecs are
immunoglobulin superfamily member lectins that selectively recognize different types and linkages of
sialic acids , which are major components of cell surface and secreted glycoconjugates . -
pubmed
Our results are consistent with a model whereby
phosphatidic acid generated from
phosphatidylcholine hydrolysis by
Spo14p regulates susceptibility to
choline - containing
lysolipid analogs and drugs . -
pubmed
A
2 - oxoglutarate - dependent dioxygenase is integrated in
DIMBOA - biosynthesis . -
pubmed
Comparing C12 with
sodium caprate (
C10 ) , used in drug products marketed , 100 micromol
C10 was needed to exert the similar absorption - enhancement of
rebamipide , a poorly absorbable antiulcer drug , to that by 10 micromol C12 , and 100 micromol
C10 was obviously more toxic to the mucosa than 10 micromol C12 . -
pubmed
Univariate and multivariate analyses predicted systolic and diastolic
sodium sensitivity based on interactions among demographic factors ( age , gender , race ,
body mass index ) and
hypertensive type [
low - renin (
LR ) , modulator ( M ) , non - modulator (
NM ) ] . -
pubmed
CONCLUSIONS : For subjects in
sodium balance , the distributions of SSS and
DSS were unimodal and the mechanisms mediating
SSS and
DSS were different . -
pubmed
OBJECTIVES :
DA-8159 is a
pyrazolopyrimidinone derivative showing potent and selective
phosphodiesterase 5 (
PDE5 ) inhibition . -
pubmed
Purification of
scotopsin and its
amino acid component . -
pubmed
Myocardial
carbohydrate metabolism during induced
cardiac arrest and
post - arrest perfusion . -
pubmed
[ Assessment of several years of the treatment with
ACTH and
corticosteroids of chronic total pelada ] . -
pubmed
Circulatory response to tilt with
hydroxyzine ,
thiethylperazine maleate ,
SA 97 . -
pubmed
The
toxicity of
tumor necrosis factor - alpha upon cholinergic neurons within the nucleus basalis and the role of
norepinephrine in the regulation of
inflammation : implications for
Alzheimer ' s disease . -
pubmed
The loss of forebrain
norepinephrine following systemic treatment with
DSP4 did not alter the level of cortical
choline acetyltransferase activity or activate microglia but significantly activated astrocytes within the basal forebrain . -
pubmed
By using artificial
polyleucine TM segments we have demonstrated in this study that a pattern of only five
amino acids ( GVxxGVxxT ) promotes specific dimerisation . -
pubmed
Single
nucleotide polymorphisms of the
IL18 gene are associated with
atopic eczema . -
pubmed
In sharp contrast with
styrylquinolines bearing a
carboxylic acid group at C-7 , these compounds proved to be inactive toward
HIV-1 integrase in in vitro assays . -
pubmed
Production of
indoleamine 2,3 - dioxygenase (
IDO ) by
tumor cells , leading to
tryptophan depletion and production of immunosuppressive metabolites , may facilitate immune tolerance of
cancer . -
pubmed
However ,
3 - HAA , but not
L - kyn markedly inhibited antigen - independent proliferation of
CD8(+ ) T cells induced by
common receptor gamma - chain cytokines IL-2 , - 7 and - 15 . -
pubmed
Our data suggest that
CD8(+ ) T cell activation induced by antigenic stimulation , a function exquisitely fulfilled by mDC , is unaffected by
tryptophan metabolites . -
pubmed
Instead , in the absence of effective T cell receptor triggering ,
3 - HAA profoundly affects homeostatic proliferation of
CD8(+ ) T cells . -
pubmed
Ferritin , the
iron storage protein , plays a key role in
iron metabolism . -
pubmed
The predicted protein contains 170
amino acid residues with a predicted molecular weight ( MW ) about 19 , 422.89 Da and theoretical isoelectric point (
PI ) of 4.73 . -
pubmed
A fusion protein containing
FcFer was produced and the purified recombinant protein exhibited similar function of
iron uptake in vitro . -
pubmed
These results suggest that protein phosphatase 1 or
2A , or both , are involved in the regulation of
Ca ( 2 + ) uptake across the plasma membrane , in exocytotic activity and in the biosynthesis of cell wall components , all processes that occur in the tip region of pollen tubes and that control pollen tube development . -
pubmed
Racecadotril , which is rapidly metabolized to its active metabolite
thiorphan , is a
neutral endopeptidase (
NEP ) inhibitor used in clinical practice for
diarrhoea with a remarkable safety profile . -
pubmed
Intraperitoneal administration of
thiorphan reduced
ibotenate - induced
cortical lesions by up to 57 % and cortical
caspase-3 cleavage by up to 59 % . -
pubmed
Thiorphan effects were mimicked by
substance P ( SP ) and , in a less potent manner , by
neurokinin A. -
pubmed
Thiorphan effects were inhibited by blockers of
NK1 and
NK2 receptors . -
pubmed
In particular , potential bioenergetic and neural mechanisms for anticonvulsant efficacy of
fluoxetine were explored using food intake /
body weight monitoring and quantification of brain
serotonin transporter protein . -
pubmed
Importantly , the 7 day period of dietary
fluoxetine exposure was effective in selectively reducing cell density in the parietal cortex and increasing
serotonin transporter protein content in the nucleus accumbens . -
pubmed
Recent genetic evidence has linked mutations in
hTHTR1 with the manifestation of
TRMA (
thiamine - responsive
megaloblastic anaemia ) , a condition also associated with
diabetes mellitus ,
sensorineural deafness and
retinal disorders . -
pubmed
Our results reveal a spectrum of mutant phenotypes , underlining that
TRMA can result from decreased
thiamine transport activity underpinned by changes in
hTHTR1 expression levels , cellular targeting and/or protein transport activity . -
pubmed
The results indicate that
manic or mixed
recurrences might rather occur at lower
lithium levels , whereas the
depressive pole prevails in the higher range . -
pubmed
The reduction of prochiral
ketones catalyzed by
Ru ( diphosphine )( diamine ) complexes has been studied at the DFT -
PBE level of theory . -
pubmed
The DFT -
PBE results suggest that this reaction is driven to the ( R)-product only by the process of binding the
acetophenone to the active site of the
trans - Ru ( H ) 2 ( S , S - dpen )( S - tolbinap ) catalyst . -
pubmed
In fact , autoantigens have been demonstrated to be content within apoptotic bodies and apoptotic cells seems to be critical in the presentation of
antigens , activation of innate immunity and regulation of macrophage
cytokine secretion . -
pubmed
The atheroprotective effect of
apo B peptide immunization occurred in the absence of an increase in peptide - specific
IgG , but was associated with an increase in
IgM recognizing native and
copper - oxidized LDL . -
pubmed
Analysis of
xenon binding to
photosystem II by X - ray crystallography . -
pubmed
Under the conditions employed , 25
Xe - binding sites were identified in each monomer of the dimeric
PSII complex . -
pubmed
One binding site was located close to the haem of
cytochrome b559 in a position analogous to a
Xe - binding site of
myoglobin . -
pubmed
The only
Xe - binding site not associated with the intrinsic subunits of
PSII was within the hydrophobic core of the
PsbO protein . -
pubmed
These altered behavioral responses to LPS were accompanied by reduction in
dopamine turnover within the striatum and increased expression of
cyclooxygenase-2 in the cortex . -
pubmed
It has been shown that
diabetes - induced
fetal abnormalities are accompanied by some metabolic disturbances including elevated
superoxide dismutase (
SOD ) activity , reduced levels of
myoinositol and
arachidonic acid and inhibition of the
pentose phosphate shunt pathway . -
pubmed
The activation of
PGU1 transcription by
galactose was found to be strain specific , independent of the
strain being an industrial or a domesticated one . -
pubmed
OBJECTIVE : To detect the release of
endostatin in microcapsules of
calcium alginate gel by emulsification - internal gelatification technology , to observe the distribution of
Endostatin microcapsules in the eye by periocular injection and biocompatibility . -
pubmed
METHODS : The
calcium alginate gel microcapsules encapsulating
endostatin were prepared by emulsification - internal gelatification technology , the release of
endostatin in microcapsules in vitro was examined by uv - Spectrophotometry . -
pubmed
RESULTS :
SDS polyacrylamide gel electrophoresis showed strap of
ES protein . -
pubmed
One chemotherapy cycle consisted of
irinotecan 180 mg / m(2 ) on days 1 and 15 ; 5 - FU 400 mg / m(2 ) bolus
IV with 600 mg / m(2 ) by a 22 hour intravenous infusion on days 1 , 2 , 15 and 16 ; and
leucovorin 20 mg / m(2 ) on days 1 , 2 , 15 and 16 , every 4 weeks . -
pubmed
Imaging was performed on a clinical 1.5 Tesla scanner , using conventional balanced gradient echo sequences ( SSFP ) ,
T2 * -weighted sequences , and
IRON - imaging . -
pubmed
To this end , a series of mononuclear
Mn ( III ) complexes was chosen for which the structures have been resolved by X - ray diffraction and the zfs parameters have been accurately determined by high - field
EPR spectroscopy . -
pubmed
Despite covering dynamic correlation contributions to the transition energies , NEVPT2 does not lead to large improvements in the results as the excitation energies of the
Mn ( III ) d - d transitions are already fairly accurate at the
SA - CASSCF level . -
pubmed
The expression of this major transcript , namely
PDCD4 (
programmed cell death gene 4 ) , was measured in beta and ductal cells after stimulation with the
incretin hormone glucagon - like peptide 1 , mitogenic
insulin , the
thiazolidinedione rosiglitazone , and by high
glucose concentrations . -
pubmed
RESULTS : The expression of the
Pdcd4 gene in pancreatic beta and ductal cells was found to be stimulated in a comparable manner by either
glucagon - like peptide 1 ,
insulin , and by high
glucose concentrations . -
pubmed
Furthermore , the
thiazolidinedione rosiglitazone specifically upregulates
Pdcd4 gene expression in beta cells in a time - dependent manner . -
pubmed
HO-2 oxidizes
heme to
CO and
biliverdin ; the latter is reduced to
bilirubin by
biliverdin reductase (
BVR ) . -
pubmed
In addition ,
HO-2 is a redox - sensitive K /
Ca ( 2 )-associated protein , and
BVR is an
S / T / Y kinase . -
pubmed
This is the first reporting of regulation of
HO-2 by
BVR and that their coordinated increase in isolated myocytes and intact heart protects against
cardiotoxicity of
beta - adrenergic receptor activation by
isoproterenol (
ISO ) . -
pubmed
In isolated cardiomyocytes , experiments with
cycloheximide , proteasome inhibitor
MG-132 , and siBVR suggested
BVR - mediated stabilization of
HO-2 . -
pubmed
Perfusion of the heart with the inhibitory peptide blocked
ISO - mediated
BVR activation and augmented apoptosis ; conversely , perfusion with the activating peptide inhibited apoptosis . -
pubmed
EGF also induces
tyrosine phosphorylation of
Toca-1 and interactions with
N - WASP and
Abi1 . -
pubmed
Interest in these
enzymes has increased in recent years due to
hydrogen fuel initiatives and concerns over development of new methods for
CO2 sequestration . -
pubmed
One
Ni - Fe enzyme called
carbon monoxide dehydrogenase (
CODH ) is a key player in the global
carbon cycle and carries out the interconversion of the environmental pollutant
CO and the greenhouse gas
CO2 . -
pubmed
AIM : To investigate the effect of
acetylcholine (
ACh ) on the
cytotoxicity of natural killer ( NK ) cells and to explore the receptor mechanisms involved in the effect . -
pubmed
The various concentrations of
ACh ,
cholinergic receptor agonists or antagonists were added to the cultures , respectively according to distinct experimental purposes . -
pubmed
The inhibitory effect of
ACh on NK cell
cytotoxicity was mimicked by
pilocarpine , an agonist of
muscarinic receptor , and by
nicotine , an agonist of
nicotinic receptor , at all applied concentrations ( 10(-10 ) - 10(-6 ) mol / L ) . -
pubmed
Muscarinic receptor antagonist
atropine blocked the inhibitory effect of
ACh on the
cytotoxicity of NK cells . -
pubmed
Nevertheless ,
tubocurarine , an antagonist of
nicotinic receptor , had no blocking effect on the suppression of NK cell
cytotoxicity by
ACh . -
pubmed
CONCLUSION :
ACh results in an inhibition of the
cytotoxicity of NK cells , and this inhibition is realized mainly through M and
N1 cholinergic receptor . -
pubmed
In addition ,
ET-1 evoked the release of
Ca ( 2 + ) which is a central regulator of oligodendrocyte differentiation . -
pubmed
Apoptosis , total
antioxide capacity ( T - AOC ) ,
acetylcholine esterase (
AchE ) and
choline acetyl transferase (
ChAT ) activities in the hippocampus and the cortex were investigated . -
pubmed
YXQNG at both doses , but not
rivastigmine , had significant reduction in apoptosis , and significant increases in T - AOC and
ChAT activity in both the hippocampus and the cortex . -
pubmed
Unlike
rivastigmine , neither dose of YXQNG showed significant reduction in
AchE activity . -
pubmed
Poly ( ADP - ribose ) polymerase-1 (
PARP-1 ) is a critical enzyme activated to facilitate DNA repair using
nicotinamide adenine dinucleotide (
NAD + ) as a substrate . -
pubmed
However , in the presence of severe oxidative stress and DNA damage ,
PARP-1 overactivation may ensue , depleting cells of
NAD + and
ATP , killing them by metabolic catastrophe . -
pubmed
Selenium glycinate supplementation increases blood
glutathione peroxidase activities and decreases
prostate - specific antigen readings in middle - aged US men . -
pubmed
Two concepts are often currently applied to
selenium in adult men in the United States : Intake is generally enough to maximize blood
glutathione peroxidase activities . -
pubmed
The hypothesis was confirmed , in that
selenium supplementation raised activities for erythrocyte and plasma
glutathione peroxidase as well as lowered values for plasma
prostate - specific antigen . -
pubmed
Cyclooxygenase-2 (
Cox-2 ) is an inducible enzyme involved in the conversion of
arachidonic acid to
prostaglandin and other
eicosanoids . -
pubmed
Several experimental and clinical studies have established potent anti -
cancer activity of NSAID ( Non - steroidal anti - inflammatory drugs ) and other
Cox-2 inhibitors such as
celecoxib . -
pubmed
The average release of mitochondrial
glutamate dehydrogenase as indicator of cell membrane damage decreased after addition of
glycine . -
pubmed
Meta - analysis : the diagnostic accuracy of
lactose breath
hydrogen or
lactose tolerance tests for predicting the North European
lactase polymorphism C / T-13910 . -
pubmed
AIM : To perform a meta - analysis of available studies that compares the north - European genetic polymorphism C / T-13910 with the
lactose breath
hydrogen and the
lactose tolerance tests , to determine their ability to predict geno / phenotype relationships . -
pubmed
METHODS : An electronic database of the literature as well as individual references in articles were searched with the theme of genetics of
lactase and comparisons with breath or
lactose tolerance tests were carried out . -
pubmed
While the introduction of
HER2 - targeted therapies , such as the monoclonal antibody
trastuzumab and the small - molecule
tyrosine kinase inhibitor
lapatinib , has significantly improved outcomes in
HER2 +
breast cancer compared with previously available therapies , use of these targeted therapies is often limited by the development of drug resistance and tolerability issues . -
pubmed
Recently , several agents have demonstrated promising activity in
HER2 + metastatic
breast cancer , either as monotherapy or in combination therapy , including the
tyrosine - kinase inhibitors
neratinib (
HKI-272 ) and
afatinib (
BIBW-2992 ) and the anti -
HER2 monoclonal antibodies
pertuzumab and
trastuzumab -
DM1 ( T -
DM1 ) . -
pubmed
Fasting serum concentrations of
glucose ( P<.05 ) and
insulin ( P<.0001 ) , and homeostasis model assessment index ( P<.0001 ) , were all higher in the low
adiponectin group than in the normal
adiponectin group . -
pubmed
OBJECTIVE : Our previous study identified
Threonine 161 (
Thr-161 ) , located in the second intracellular loop of the
delta - opioid receptor (
DOR ) , as the only consensus phosphorylation site for
cyclin - dependent kinase 5 (
Cdk5 ) . -
pubmed
The aim of this study was to assess the function of
DOR phosphorylation by
Cdk5 in complete Freund 's adjuvant ( CFA)-induced inflammatory
pain and
morphine tolerance . -
pubmed
RESULTS : Both the total amount and the surface localization of
pThr-161 -
DOR were significantly enhanced in the ipsilateral DRG following CFA injection . -
pubmed
We report that formation of
peroxynitrite ( PN ) in response to activation of
nitric oxide synthases and
NADPH oxidase in spinal cord contributes to neuropathological changes through two mechanisms . -
pubmed
Targeting
PN with
PN decomposition catalysts ( PNDCs ) not only blocked the development of
paclitaxel - induced
neuropathic pain without interfering with antitumor effects , but also reversed it once established . -
pubmed
The improved chemical design of
SRI6 and
SRI110 also affords selectivity for
PN over other reactive
oxygen species ( such as
superoxide ) . -
pubmed
Our findings identify
PN as a critical determinant of
CIPN , while providing the rationale toward development of
superoxide - sparing and " PN - targeted " therapeutics . -
pubmed
Discovery of
XL413 , a potent and selective
CDC7 inhibitor . -
pubmed
CDC7 is a
serine /
threonine kinase that has been shown to be required for the initiation and maintenance of DNA replication . -
pubmed
In this paper , we disclose the discovery of a potent and selective
CDC7 inhibitor ,
XL413 ( 14 ) , which was advanced into Phase 1 clinical trials . -
pubmed
The full - length cDNA of Ec -
TRX was comprised of 767bp with a 327bp open reading frame that encodes a putative protein of 108
amino acids . -
pubmed
The
PSAP data were used to infer the mass of light absorbing
carbon ( LAC ) and the daily mean varied from 0.1 to 6.8 mug m-3 . -
pubmed
VEGF regulates various endothelial cellular processes , including cell migration , proliferation , and survival , through the
serine threonine protein kinase
Akt . -
pubmed
Graphene nanosheets (
GNS ) were employed as an air electrode for a
sodium - air battery ( SAB ) . -
pubmed
The mean age and duration of
alcohol use were 47.9 + -7.4 and
16.1 + -5.2 years , respectively . -
pubmed
With the protection of
Methylprednisolone , the upregulation of
PUMA and
p53 expression as well as the decrease of
MDM2 and
SirT2 in spinal cord were partially rescued in western bolt analysis . -
pubmed
This association remained statistically significant for
glucose , total
cholesterol , and
low - density lipoprotein after controlling for confounding factors in multivariate analysis . -
pubmed
The protein expression of
UBC9 , as compared with other biomarkers , showed an inverse correlation with
steroid hormones (
ER ,
progesterone ( PgR ) ) ,
BRCA1 ,
p27 ,
p21 and
MDM4 , and a positive correlation with triple negative , basal
cytokeratins (
CK14 and
CK5 / 6 ) ,
epidermal growth factor receptor (
EGFR ) , basal - like
breast cancer phenotype ,
p53 ,
phosphoinositide-3 - kinases (
PI3KCA ) ,
placental cadherin , (
P - cadherin ) and
BRCA1 regulators (
metastasis tumor antigen-1 (
MTA1 ) . -
pubmed
Track - etched
polyethylene terephthalate (
PET ) membranes with previously introduced aliphatic
C - Br groups as initiator on the pore surface were used to prepare first a functional polymer scaffold , grafted
poly ( methacrylic acid ) , via surface - initiated atom transfer radical polymerization (
SI - ATRP ) . -
pubmed
After template protein (
IgG ) binding to the scaffold , UV - initiated cross - linking copolymerization of
acrylamide and
methylenebisacrylamide (
MBAA ) as second step lead to a grafted
MIP hydrogel layer . -
pubmed
The influences of the three independent parameters , scaffold chain length by
SI - ATRP time , degree of cross - linking of the
MIP layer by
MBAA content , and grafted
MIP layer thickness by UV irradiation time , were studied to optimize protein binding capacity and selectivity . -
pubmed
The main inhibitory neurotransmitter ,
GABA , acts on both ligand - gated and G protein - coupled receptors , the
GABAA / C and GABAB receptors , respectively . -
pubmed
Low concordance and resistance mutation emergence in the
HIV protease gene among circulating and cell - associated viruses at viral replication episodes during
darunavir /
ritonavir monotherapy . -
pubmed
Because
Eotaxin-3 - EGFP and
ssEGFP ( small soluble cargo proteins ) were largely depleted from
post - fusion WPBs , we studied these molecules in cells preincubated in the weak base
NH4Cl which caused WPB alkalinisation and rounding similar to that produced by plasma membrane fusion . -
pubmed
MT plays a critical role in
zinc sequestration in the liver suggesting its therapeutic effect would be mediated by
zinc . -
pubmed
Zinc supplementation was introduced 4 weeks after BDL surgery via intragastric administration once daily for 2 weeks resulting in a significant reduction in the
collagen deposition in the liver and an increase in the survival rate . -
pubmed
Furthermore ,
zinc suppressed gene expression of
alpha - SMA and
collagen I and enhanced the capacity of
collagen degradation , as determined by the increased activity of total
collagenases and elevated mRNA and protein levels of
MMP13 . -
pubmed
Therefore , the results demonstrate that
zinc supplementation suppresses BDL - induced
liver fibrosis through both inhibiting
collagen production and enhancing
collagen degradation . -
pubmed
Compared with ACSF - exposed animals ,
SCI - elevated [
H2O2 ] significantly increased intracellular protein and DNA oxidation by threefold and
MLP by eightfold in neurons , respectively . -
pubmed
H2O2 also significantly increased cell loss and the numbers of terminal deoxynucleotidyl transferase ( TdT)-mediated
deoxyuridine triphosphate-(
dUTP)-
biotin nick end labeling ( TUNEL)-positive and active
caspase-3 - positive neurons by 2.3- , 2.8- , and 5.6 - fold compared to ACSF controls , respectively . -
pubmed
Our results directly and unequivocally demonstrate that
SCI - elevated [
H2O2 ] contributes to
post - SCI MLP , protein , and DNA oxidation to induce cell death . -
pubmed
Therefore , we conclude that ( 1 ) the role of
H2O2 in secondary
SCI is pro - oxidation and pro - cell
death , ( 2 ) apoptosis is a pathway for
SCI - elevated [
H2O2 ] to induce cell
death , ( 3 )
caspase activation is a mechanism of
H2O2 - induced apoptosis after
SCI , and ( 4 )
MnTBAP treatment significantly decreased
H2O2 - induced oxidation , cell loss , and apoptosis to the levels of ACSF controls , further supporting
MnTBAP 's ability to scavenge
H2O2 by in vivo evidence . -
pubmed
The
dopamine receptor sensitization is followed , after
imipramine discontinuation , by an opposite phenomenon (
dopamine receptor desensitization ) and an increased immobility time (
depression ) in the forced swimming test of
depression . -
pubmed
The molecular architecture of
PaMTH1 and the mechanism of the
methyl transfer reaction remain unknown . -
pubmed
Here , we report the crystal structures of
PaMTH1 apoenzyme ,
PaMTH1 - SAM ( co - factor ) , and
PaMTH1 -
S - adenosyl homocysteine ( by - product ) co - complexes refined to 2.0 , 1.9 , and 1.9 A , respectively . -
pubmed
Furthermore , using NMR , mass spectrometry , and site - directed active site mutagenesis , we show that
PaMTH1 catalyzes the transfer of the
methyl group from
SAM to one
hydroxyl group of the
myricetin in a cation - dependent manner . -
pubmed
genitalium with
macrolide resistance - associated 23S rRNA mutations and
fluoroquinolone resistance - associated
parC alterations were 47.1 % and 36.8 % , respectively . -
pubmed
In the
era of increasing resistance to antibiotics , the efficacy of
fluoroquinolone prophylaxis and standard treatment of
febrile neutropenia have been questioned . -
pubmed
Post - hoc , observed - case analysis included pooled data from six studies on 40 - 160 mg / day
lurasidone ; two studies included active comparators ( 2 - 6 mg / day
risperidone or 200 - 800 mg / day
quetiapine XR ) . -
pubmed
AIM : A contralateral
breast cancer (
CBC ) is
today treated as an independent primary
tumour , although recent data suggest risk and prognosis of
CBC to be influenced by characteristics of and treatment given for the first
tumour ( BC1 ) . -
pubmed
Crayfish visceral and tail tissue , as well as water - deployed LDPE , were extracted and analyzed for 62
PAHs using
GC -
MS /
MS . -
pubmed
Polydatin Protecting Kidneys against
Hemorrhagic Shock - Induced
Mitochondrial Dysfunction via
SIRT1 Activation and
p53 Deacetylation . -
pubmed
Beneficial effect of PD was abolished partially when the
SIRT1 inhibitor
Ex527 was added . -
pubmed
The transepithelial electrical resistance ,
MTT (
3 -( 4,5 - dimethyl-2 - thiazolyl )- 2,5 - diphenyltetrazolium bromide ) assay , and
lactate dehydrogenase (
LDH ) release rate were investigated . -
pubmed
The
MTT assay revealed that the cells recovered their
mitochondrial dehydrogenase activity without proliferation . -
pubmed
These results suggest that the damage by
MCFA compounds is recoverable and that the recovery can be assessed by an
MTT assay , but that the
LDH - release behavior is different among the enhancers . -
pubmed
Isoliquiritigenin suppresses
tumor necrosis factor - alpha - induced
inflammation via
peroxisome proliferator - activated receptor - gamma in intestinal epithelial cells . -
pubmed
A signal transduction study revealed that
ISL significantly attenuates
TNF - alpha - mediated
JNK phosphorylation . -
pubmed
ISL - induced
ERK1 / 2 phosphorylation was associated with
PPARgamma expression . -
pubmed
Collectively ,
ISL - induced
PPARgamma mediated , at least partially , the suppression of intestinal
inflammation . -
pubmed
We precisely controlled the
MOS interface for
diamond by wet annealing and fabricated p - channel and planar - type MOSFETs with
phosphorus - doped n - type body on
diamond ( 111 ) substrate . -
pubmed
Detailed microscopic analysis shows that the
NTE relies on strong intralayer
Ti - O covalent bonding and weaker interlayer interactions , which is in contrast to conventional
NTE mechanisms for
perovskites , such as rigid - unit modes , structural transitions , and electronic or magnetic ordering . -
pubmed
Birds fed the lowest level of dietary
lysine ( 1.016 % ) showed a lower expression of genes such as
NADH dehydrogenase subunit I (
ND1 ) ,
cytochrome b (
CYTB ) and
cytochrome c oxidase subunits I (
COX I ) ,
II (
COX II ) and III (
COX III ) , displaying the worst performance and body protein deposition . -
pubmed
Several hypotheses can be proposed for the molecular mechanisms of the protection conferred by
DRB1 * 13 , some of which can explain the dominant effect of
DRB1 * 13 molecules over the susceptible alleles , but the actual protective function of
DRB1 * 13 requires further study . -
pubmed
Arsenic accumulation and speciation were analyzed using inductively coupled plasma - mass spectrometry ( ICP -
MS ) . -
pubmed
Identification of residues that control
Li + versus
Na + dependent
Ca2 + exchange at the transport site of the mitochondrial
NCLX . -
pubmed
BACKGROUND : The
Na +/ Ca2 +/ Li + exchanger (
NCLX ) is a member of the
Na+/Ca2
+ exchanger family . -
pubmed
The metabolites showed positive correlations with total- and
LDL -
cholesterol ( r ranged from 0.13 to 0.57 ) . -
pubmed
When additionally adjusting for total- ,
LDL- and
HDL -
cholesterol ,
triglycerides and
C - reactive protein ,
acyl - alkyl - phosphatidylcholine C36:3 and
diacyl - phosphatidylcholines C38:3 and C40:4 remained associated with risk of
MI . -
pubmed
In this study , a thermosensitive
butane diisocyanate (
BDI)-
collagen hydrogel ( BC hydrogel ) was designed as an injectable cell delivery carrier of tendon stem / progenitor cells ( TSPCs ) for tendon tissue engineering . -
pubmed
It was significant correlation between
EMAP - II and
HbAc1 , blood
glucose , total
cholesterol ,
LDL ,
triglycerides and inverse correlation between
EMAP - II and
HDL , endotheliumdependent dilatation . -
pubmed
The revealed change of
EMAP - II serum level reflects an endothelial dysfunction in
type 1 diabetes , alteration of
carbohydrate and lipid metabolism could influence of this pathway . -
pubmed
OBJECTIVE : To evaluate the effects of
CCB on EF we have performed a meta - analysis of available data from randomized and placebo - controlled or other treatment - controlled clinical studies encompassing effects of
CCB on EF , as measured by flow - mediated dilation (
FMD ) of the brachial artery . -
pubmed
CCB improved
FMD more pronounced than
thiazide diuretics - TD ( 3 studies , 157 participants , WMD=2.08 % , 95 % CI=0.35 - 3.80 % ; P=0.02 ) . -
pubmed
Oppositely ,
ACE inhibitors ( ACEI ) and
angiotensin receptor blockers ( ARB ) notably improved
FMD if compared to
CCB ( CCB vs. ACEI : 5 studies , 533 participants , WMD = -1.62 % , 95 % CI = -2.74 % to -0.50 % ; P=0.005 ; and CCB vs. -
pubmed
Evidence for the synthesis of an unusual high spin ( S = 7/2 ) [
Cu-3Fe-4S ] cluster in the radical - SAM enzyme
RSAD2 (
viperin ) . -
pubmed
We demonstrate the synthesis and spectroscopic characterization of an unusual high spin ( S = 7/2 ) [
Cu-3Fe-4S ] cluster in a member of the
radical - SAM enzymes . -
pubmed
52.6 % ( n = 72 ) of them were treated with
dementia medication :
AchEIs 16.1 % ( n = 22 ) ,
memantine 9.5 % ( n = 13 ) , antidepressants 23.4 % ( n = 32 ) , antipsychotic medication 9.5 % ( n = 13 ) , tranquilisers 16.8 % ( n = 23 ) and anticonvulsant drugs 6.6 % ( n = 9 ) . -
pubmed
The enzymatic hydrolysis of chitin , one of the most abundant
carbohydrates in nature , is achieved by
chitinases , enzymes of increasing importance in biomedicine and industry . -
pubmed
Unlike most retaining
glycosidases , family GH18
chitinases follow a substrate - assisted mechanism in which the
2 - acetamido group of one
N - acetylglucosamine monomer , rather than a basic residue of the
enzyme , reacts with the
sugar anomeric
carbon , forming an intermediate that has been described as an
oxazolinium ion . -
pubmed
The interpretation of these results as an evidence for the
RO5263397 anticompulsive - like action , however , should be taken with caution because the drug also influenced the drinking behavior and only weakly affected the other parameters of
SIP used to reveal the potential anticompulsive - like effects of drugs . -
pubmed
The chromatographic separation was achieved on a Waters
HSS T3 column under gradient elution with
acetonitrile and water containing
formic acid as the mobile phase . -
pubmed
PSI has the potential to generate reactive
oxygen species and be oxidatively inactivated by the reactive
oxygen species . -
pubmed
It has been reported that
PSI photoinhibition does not occur as long as the reaction - center
chlorophyll ( P700 ) remains oxidized , even in excess light conditions . -
pubmed
PSI photoinhibition correlated linearly with decreases in effective quantum yield of PSII and nonphotochemical quenching ; however , the photosynthetic
CO2 assimilation rate was less affected , as exemplified by ~50 % of the normal
CO2 assimilation rate maintained with an 80 % loss in
PSI photochemical activity . -
pubmed
Based on these results , we propose that the stoichiometric quantity of
PSI is optimized to induce P700 oxidation for dissipating excess light energy in
PSI , thus avoiding inhibition of photosynthetic
CO2 assimilation caused by PSI photoinhibition . -
pubmed
Simple linear regression models revealed that
ammonia - oxidizing archaea ( AOA ) contributed much to
N2O emission (
R2 = 0.77 ) . -
pubmed
Additionally , the
R2 coefficient of linear regression was 0.87 between
nosZ genes and
N2O emission rates in alpine meadow . -
pubmed
Novel antimycobacterial
C-21 amide derivatives of the antibiotic
fusidic acid : synthesis , pharmacological evaluation and rationalization of media - dependent activity using molecular docking studies in the binding site of
human serum albumin . -
pubmed
The
fusidic acid ethanamides were , generally , the most potent of the analogues evaluated for antimycobacterial activity ( MIC90 & lt ; 10 muM ) in the MB7H9 /
CAS medium . -
pubmed
The lack of activity in the MB7H9 /
ADC medium was supported by strong binding interactions in the
fusidic acid binding site of the
human serum albumin (
HSA )
protein . -
pubmed
Doxorubicin and liposomal
doxorubicin induce senescence by enhancing
nuclear factor kappa B and mitochondrial membrane potential . -
pubmed
KEY FINDINGS : Based on the results , the highest doses of
Dox and Ldox ( 0.75 and 0.1 mg / kg / BW respectively ) significantly increased the level of inflammatory markers and according to other factors especially
p53 and
SA beta - gal expression , both were able to induce senescence but the changes in Ldox were less tangible than the
Dox . -
pubmed
Identification of
Aloperine as an anti - apoptotic
Bcl2 protein inhibitor in
glioma cells . -
pubmed
In addition , molecular docking demonstrated that
ALO expectedly docks into the active pocket of the
Bcl2 protein , suggesting
Bcl2 as a direct target of
ALO . -
pubmed
In addition ,
Bcl-2 overexpression could rescue
ALO - induced
Bcl-2 inhibition and suppress pro - apoptotic effects in
glioma cells . -
pubmed
Conclusion : Taken together , these findings suggest that the natural agent
ALO effectively enhances apoptosis by acting as a potential
Bcl2 inhibitor in
human glioma cells . -
pubmed
In this study , three - dimensional quantitative structure - activity relationship ( 3D - QSAR ) models of
PPARalpha receptor (
CoMFA-1 , q2 = 0.636 , r2 = 0.953 ;
CoMSIA-1 , q2 = 0.779 , r2 = 0.999 ) and
PPARdelta receptor (
CoMFA-2 , q2 = 0.624 , r2 = 0.906 ; CoMSIA-2 , q2 = 0.627 , r2 = 0.959 ) were successfully constructed using 35
triazolone ring derivatives . -
pubmed
In the present study , we developed an environmentally friendly and convenient method to decorate the wood fiber /
polypropylene ( WF /
PP ) composite board . -
pubmed
We used
chlorinated polypropylene (
CPP ) as an intermediate film to laminate wood veneer to WF /
PP composite boards by hot - pressing at 110 C and then cooling down . -
pubmed
Vitamin C is incorporated into the cerebrospinal fluid (
CSF ) through choroid plexus cells . -
pubmed
While the transfer of
vitamin C from the blood to the brain has been studied functionally , the
vitamin C transporter ,
SVCT2 , has not been detected in the basolateral membrane of choroid plexus cells . -
pubmed
The effect of energy level alignment between the hole transport layer (
HTL ) and active layer in
PbS quantum dot ( QD ) solar cells was investigated . -
pubmed
Our results indicated that
DEHP is cytotoxic ; it induces the overexpression of
Hsp70 protein and causes oxidative damage through the generation of free radicals leading to lipid peroxidation induction and the increase of
superoxide dismutase (
SOD ) and
catalase (
CAT ) activities . -
pubmed
In addition , cell treatment with
DEHP resulted in a
glutathione (
GSH ) content decrease and a decrease in the
glutathione reductase (
GR ) activity . -
pubmed
As new evidence provided in this study , we demonstrated that the
DEHP affected the two enzymes ' activities of the oxidative phase of the
pentose phosphate pathway :
Glucose-6 - phosphate dehydrogenase (
G6PD ) and
6 - phosphogluconate dehydrogenase (
6PGD ) . -
pubmed
This leads to a decrease in the level of
NADPH used by the
GR to maintain the regeneration of the
reduced GSH . -
pubmed
PBS and
HPBS are a type of biological products detected on the
C - S bond cleavage step through various oxidative biodesulfurization ( OBDS ) pathways , and are useful synthetic intermediates and fine chemicals . -
pubmed
A stabilized power conversion efficiency ( PCE ) of
16.1 % under simulated
AM 1.5 G 1 sun illumination with an aperture of 1.00 cm2 was achieved for PSCs using an additive - free
spiro - OMeTAD layer cast from PC . -
pubmed
Molecular mechanism for bidirectional regulation of
CD44 for lipid raft affiliation by palmitoylations and
PIP2 . -
pubmed
The process is controlled by two different pathways : On the one hand palmitoylation keeps
CD44 in lipid raft domains and disables the linking to the
cytoplasmic adaptor , whereas on the other hand , the presence of
phosphatidylinositol-4,5 - biphosphate (
PIP2 ) lipids accelerates the formation of the
CD44 - adaptor complex . -
pubmed
The molecular mechanism explaining how
CD44 is migrating into and out of the lipid raft domains and its dependence on both palmitoylations and the presence of
PIP2 remains , however , elusive . -
pubmed
We observe a delicate balance between the influence of the palmitoylations and the presence of
PIP2 lipids : whereas the palmitoylations of
CD44 increases the affinity for raft domains ,
PIP2 lipids have the opposite effect . -
pubmed
We find that the presence of
PIP2 lipids allows
CD44 and
FERM to associate in an experimentally observed binding mode whereas the highly palmitoylated species shows no binding affinity . -
pubmed
OBJECTIVE : Combined
methylmalonic acidemia and
homocysteinemia is a genetically heterogeneous disorder of
cobalamin (
cbl ;
vitamin B12 ) metabolism , which consists of five subtypes including
cblC ,
cblD ,
cblF ,
cblJ , and
cblX deficiencies . -
pubmed
Changes in the osmolality and level of
angiotensin II (
ANG II ) are important peripheral signals modulating appropriate central sympathetic output and maintaining a normal arterial pressure during high salt intake . -
pubmed
The median preoptic nucleus ( MnPO ) receives reciprocal inputs from the subfornical organ ( SFO ) and organum vasculosum of the lamina terminalis ( OVLT ) , the circumventricular organs that have been shown to be necessary in multiple central effects of changes in the osmolality and circulating
ANG II directed toward the maintenance of
sodium and water homeostasis . -
pubmed
Femoral venous catheters were also implanted to collect blood for the measurements of plasma osmolality and
sodium concentration , as well as
plasma renin activity . -
pubmed
Identification of a novel homozygous variant in the
alkaline phosphate (
ALPL ) gene associated with
hypophosphatasia . -
pubmed
IgG and
IgG2a titers elicited by the Ace -
DEX MP platform were compared to titer levels elicited by
MSP2 adsorbed to an FDA - approved alum adjuvant , MSP2 alone , and
PBS alone . -
pubmed
Both adsorption of
MSP2 to Ace - DEX MPs and to alum elicited antibody responses in vivo , but only the formulation containing Ace -
DEX MPs was able to elicit a significant Th1 - biased response needed to combat the intracellular pathogen . -
pubmed
As such ,
MSP2 adsorbed to Ace -
DEX MPs demonstrates promise as a
malaria vaccine . -
pubmed
Compared with the placebo , probiotic and
selenium co - supplementation resulted in a significant elevation in total antioxidant capacity ( beta 91.09 mmol / L ; 95 % CI , 35.89 , 146.30 ; P = 0.002 ) and total
glutathione ( beta 96.50 mumol / L ; 95 % CI , 26.13 , 166.87 ; P = 0.008 ) and a significant reduction in high - sensitivity
C - reactive protein levels ( beta - 1.44 mg / L ; 95 % CI , - 2.22 , - 0.66 ; P = 0.001 ) . -
pubmed
Additionally , co - supplementation significantly decreased fasting
glucose ( beta - 7.40 mg / dL ; 95 % CI , - 10.15 , - 4.64 ; P < 0.001 ) ,
insulin levels ( beta - 1.46 muIU / mL ; 95 % CI , - 2.35 , - 0.57 ; P = 0.002 ) , and homeostasis model of assessment -
insulin resistance ( beta - 0.51 ; 95 % CI , - 0.72 , - 0.29 ; P < 0.001 ) and a significant increase in quantitative
insulin sensitivity check index ( beta 0.01 ; 95 % CI , 0.006 , 0.01 ; P < 0.001 ) compared with the placebo . -
pubmed
It was found that it amplified the induction of liver
tyrosine aminotransferase by
dexamethasone markedly with doses of
dexamethasone that caused minimal
enzyme induction , but had little effect with doses that caused maximal induction . -
pubmed
These results and the finding that
actinomycin D inhibited the amplification indicate that the amplifier does not act on the translational level of
enzyme induction . -
pubmed
It was found that the amplifier increased both incorporation of
[ 3H ] dexamethasone into the cytosol and binding of
[ 3H ] dexamethasone of
cytosol protein and that it decreased decay of the
[ 3H ] dexamethasone - protein complex . -
pubmed
sIg+ lymphocytes detected by immunofluorescence and by
51Cr release
cytotoxicity showed similar frequency and adherence properties . -
pubmed
Increased precipitability of
IgG and
C4 was seen in a great percentage ( 80 % ) of pathological sera giving positive
PEG precipitation without direct influence of
IgG ,
IgM ,
C1q ,
C3 and
C4 serum levels . -
pubmed
The
C1q binding test could be replaced by the more direct and simple evaluation of the amount of
C4 precipitated with
IgG by 3.5 %
PEG . -
pubmed
The lung is the site of a number of reactions affecting vasoactive substances : hydrolysis of
angiotensin I ,
bradykinin and
ATP , occurring at the surface of endothelial cell ; uptake and intracellular metabolism of
5 - hydroxytryptamine and
norepinephrine ; and intracellular oxidation of
prostaglandins . -
pubmed
Pretreatment of granulosa cells with
activin , but not
FSP , for 24 h increased the responsiveness of cells to
FSH ( 20 ng / ml ) and
LH ( 40 ng / ml ) shown by increases in
aromatase activity ,
progesterone , and immunoreactive
inhibin production over and above control in a manner which depended upon
activin doses . -
pubmed
Absorption and circular dichroism spectra of stable
enzyme - substrate intermediates of
aspartate aminotransferase were recorded at subzero temperatures ( down to -65 degrees C ) in the cryosolvent water /
methanol . -
pubmed
The intermediates were formed either between the
pyridoxal form of the
enzyme and its
amino acid substrates , or between the
pyridoxamine form and its
oxo acid substrates . -
pubmed
Accumulation of the
ketimine in the reverse direction would indicate that the proton abstraction at
C4 ' is rate - limiting in this half - reaction . -
pubmed
on the therapeutic level : the attacks resist
barbiturate and
benzodiazepin treatment in 80 % of the cases , but the attacks diminish as the
diabetes is brought under control through
insulin treatment . -
pubmed
There is evidence that a functional deficit of
serotonin /
noradrenaline and/or of their precursors
L - tryptophan (
L - TRP )/ tyrosine and disorders in the hypothalamic - pituitary - adrenal (
HPA ) axis are linked to the pathophysiology of severe
depressions . -
pubmed
Above indications were all decreased ; especially both the thymus weight and the thymus
ADA activity were markedly decreased in the
zinc deficient group . -
pubmed
After
zinc supplementation for one month , all the
body weight , weight of the thymus and spleen , and
ADA activity of the spleen were recovered to the control group 's level , while
ADA activity of the thymus was 33.03 % higher than that of the control group . -
pubmed
Our data suggested that
zinc was not only an important trace element in maintaining the growth of the body , the development of the thymus and spleen , but it may be an important agent to activate the
ADA or to prompt synthesis of
ADA . -
pubmed
specificity of the 5 laboratory values AP ,
GGT ,
GPT ,
GOT and
bilirubin was 22 % resp . -
pubmed
The diagnostic value determined by their Chi2 - value was AP greater than
GPT greater than
bilirubin greater than GOT greater than
GGT . -
pubmed
The combination of AP ,
GPT and
bilirubin with the result of abdominal sonography in a logistic discriminant function led to a correct a posteriori classification of all patients . -
pubmed
The
serine esterase affinity label
diisopropyl fluorophosphate reacts strongly with
granzymes A and D , to a lesser extent with B , E , F , G , and H , and not at all with C and
F. -
pubmed
Quaternary forms of classical muscarinic antagonists distinguish subpopulations of
muscarinic receptors : correlation with
gallamine - defined subpopulations . -
pubmed
Under the same conditions , the inhibition by
N - methylatropine (
NMA ) and
N - methylscopolamine (
NMS ) of the binding of
[ 3H ] QNB is consistent with the presence of subpopulations of
receptors that differ greatly in affinities toward these quaternary ligands . -
pubmed
Thyroxine ,
triiodothyronine ,
thyroglobulin , hypophyseal thyrotrophic and gonadotrophic hormone ,
prolactin ,
cortisol and
corticotrophin concentrations in the peripheral blood of 475 healthy females were measured by radioimmunoassay at different times of their arrival to the North . -
pubmed
The role of
adrenergic receptors in
Ni2 + -induced coronary vasoconstriction . -
pubmed
The experiments on both models showed that ( i ) alfa - adrenergic blockade by
phenoxybenzamine or
phentholamine caused only partial depression of
Ni2 + -induced coronary vasoconstriction : ( ii )
beta - adrenergic receptor blockade by
propranolol totally prevented
Ni2 + -action , and ( iii )
Ni2 + ( 1 microM ) caused significant inhibition of coronary vasodilatation induced by
isoproterenol . -
pubmed
The experimental results indicate that
alfa - adrenoceptors play minor role ( if any ) in the coronary action mechanism of
Ni2 + but it may be mediated by beta - adrenergic mechanisms . -
pubmed
Nickel was found to alter the reactivity of coronary
beta - adrenoceptors suggesting a possible modulatory role of this trace metal in coronary adrenergic mechanisms . -
pubmed
Finally , we show that high
MBA gels are also effective in resolving mixtures of
oligoribonucleotides such as those produced by
T1 ribonuclease digestion of small RNAs . -
pubmed
Comparison of
gonadotropin releasing hormone and
human chorionic gonadotropin and effects of
progestogen and
estrogen . -
pubmed
The demethylating enzyme activity is inducible by
phenobarbital (
PB ) , not by
3 - methylcholanthrene (
3 - MC ) . -
pubmed
Pretreatment of animals with
PB plus
3 - MC resulted in reduced inducibility compared with
PB - pretreatment alone . -
pubmed
Probably this is not due to an inhibition of
PB - induction by
3 - MC , but effected by
PB residues in the liver tissue and in the incubation mixture . -
pubmed
The effector cells are insensitive to
anti - Thy 1 antibody and complement treatment , are not H-2 restricted , do not adhere to plastic and are unaffected by
silica or carrageenan . -
pubmed
The treatment results were evaluated by thyroid scintigraphy and sonography , by
triiodothyronine ,
thyroxine , and
thyrotropin assays , and by a
thyrotropin - releasing hormone stimulation test 6 months after the end of treatment . -
pubmed
Growth hormone (
GH ) suppresses its own secretion by stimulating
somatostatin (
SRIF ) release . -
pubmed
Our results show that
alpha - amanitin blocks cells specifically in
G1 , irrespective of the concentration within the range of 3 to 30 micrograms / ml . -
pubmed
This suggests that cells exposed to
alpha - amanitin are able to complete their cell cycle because sufficiently high levels of mRNA are present in S /
G2 due to basal level transcription , or are left from preceding cell cycles . -
pubmed
Serum
testosterone concentration was higher before the race at altitude than at sea level ( 19 % , P & lt ; 0.02 ) , and 30 min after the race
growth hormone (
GH ) concentration was higher at sea level than at moderate altitude ( P & lt ; 0.002 ) . -
pubmed
This was followed by in situ hybridization using
35S - labeled riboprobes for
PRL and
GH mRNA . -
pubmed
Incorporation of these
fatty acids leads to profound changes in membrane
fatty acid composition which in turn may alter the activity of
transmembrane receptor / effector systems . -
pubmed
It is suggested that
beta - adrenoreceptor number , subtype and
adenylyl cyclase stimulation may be regulated by alterations in membrane
fatty acid composition as a result of changes in the levels of
polyunsaturated fatty acids and alterations in
eicosanoid production . -
pubmed
The most evaluated regimens include dual therapy with a
proton pump inhibitor and either
amoxicillin or
clarithromycin , and
bismuth - based triple therapy with
metronidazole and
tetracycline . -
pubmed
Moreover , combinations of a
proton pump inhibitor and
clarithromycin show more predictable and higher eradication rates than
amoxicillin combinations . -
pubmed
Actual
pentamidine dose delivered by Respigard
II nebulizer . -
pubmed
The Respigard
II nebulizer system is the approved method to deliver
pentamidine aerosols in the USA . -
pubmed
Pentamidine solutions ( 300 mg in 6 mL water ) were nebulized continuously with the Respigard
II nebulizer system until dryness ( 40 min operation ) . -
pubmed
SS receptors were identified using
125I - Tyr(11)-SS and isolated pancreatic acinar membranes in vitro . -
pubmed
The aim of this study was to characterize the changes in the quantitative expression of
beta 2 - integrins and
L - selectin detected by means of
fluorochrome - conjugated monoclonal antibodies and flow cytometry on leukocytes in the systemic circulation after a major
musculoskeletal trauma , i.e. -
pubmed
The cells on the
PPy - coated
ITO plate could be kept in culture , without any significant changes in morphology and in the secretory responsiveness to
acetylcholine as compared with those of the cells cultured on
collagen . -
pubmed
This paper describes that
PPy films are applicable as a polymer - modified electrode which support the cell function without
collagen . -
pubmed
A combination of
acyclovir and
interferon therapy was started at 25 weeks . -
pubmed
[ Glycogenolysis through
lyase reaction and
1,5 - anhydroglucitol ] . -
pubmed
The phosphorylation of a
serine residue at position 133 of
CREB is critical for activation of
CREB . -
pubmed
It is initiated by a highly local , rise in
calcium ion concentrations near the cell membrane , but culminates in the activation of a specific
calmodulin - dependent kinase known as
CaMK IV , which is constitutively present in the neuronal nucleus . -
pubmed
Certain
Ca2 + entry systems ( L - type
Ca2 + channels and
NMDA receptors ) are able to cause mobilization of
calmodulin , whereas others ( N- and P / Q - type
Ca2 + channels ) are not . -
pubmed
This translocation of
calmodulin provides a form of cellular communication that combines the specificity of local
Ca2 + signalling with the ability to produce action at a distance . -
pubmed
However , all recombinant viruses contained an insertion of two
amino acids between codons 68 and 69 of
RT as well as an
amino - acid change at codon 67 , as was seen in the serum - derived viruses . -
pubmed
CONCLUSIONS : Antiretroviral therapy including
zidovudine may yield replicating viruses with a two
amino - acid insertion in
RT in combination with
amino - acid changes at codons 67 and 215 , which are highly resistant to
lamivudine and
stavudine on top of
zidovudine and have unpredictable susceptibility to
didanosine and
zalcitabine despite lack of previously reported corresponding resistance - associated
amino - acid changes . -
pubmed
The same features were found in all four
strains of the genus Fibrobacter metabolizing simultaneously
glucose and
cellobiose : i ) differential metabolism of
glucose and
cellobiose ;
glucose seems preferentially used for glycogen storage and energy production , while part of
cellobiose seems to be diverted from glycolysis , ii ) synthesis of
cellodextrins , mainly from
cellobiose not entering into glycolysis , iii ) accumulation of
glucose 6 - phosphate , iv ) simultaneous presence of
cellobiose phosphorylase and
cellobiase activities . -
pubmed
Many of axon terminals of striatonigral neurons were confirmed
electron microscopically not to be in synaptic contact with
SPR - like immunoreactive structures within the striatum . -
pubmed
p62 is an only recently discovered protein that is a
phosphotyrosine - independent ligand of the SH2 domain of
p56 ( lck ) , a member of the
c - src family of cytoplasmic kinases . -
pubmed
Average systolic peak velocity ( ASV ) and deceleration time of diastolic flow velocity (
DDT ) significantly correlated to 1 - month A - WMSI ( r=-0.54 , P=0.007 and r=-0.62 , P=0.002 , respectively ) , and optimal cutoff values to predict viable myocardium ( defined as 1 - month A - WMSI & lt;/=2.0 ) were 6.5 cm / s for ASV and 600 ms for
DDT ( sensitivity=0.79 , specificity=0.89 and sensitivity=0.86 , specificity=0.89 , respectively ) . -
pubmed
Fertility was measured by serum
progesterone ( P(4 ) ) concentrations and pregnancy diagnosis by rectal examination
post - insemination . -
pubmed
They were questioned regarding risk factors and blood samples were taken for
HBsAg ,
anti - HBs and anti -
HBc testing . -
pubmed
Development of the antigen retrieval (
AR ) technique , a simple method of boiling archival paraffin - embedded tissue sections in water to enhance the signal of immunohistochemistry ( IHC ) , was the fruit of pioneering efforts guided by the philosophy of rendering IHC applicable to routine
formalin - fixed ,
paraffin - embedded tissues for wide application of IHC in research and clinical pathology . -
pubmed
Nanoelectrospray ionization mass spectrometry ( nanoESI -
MS ) and computer simulation were applied to the characterization of non - covalent interactions of
[ Leu5 ]- enkephalin (
LE ) and its optical isomers ,
[ D - Tyr1 , Leu5 ]- enkephalin (
Y - LE ) ,
[ D - Phe4 , Leu5 ]- enkephalin (
F - LE ) and
[ D - Tyr1 , D - Phe4 , Leu5 ]- enkephalin (
YF - LE ) . -
pubmed
The dimer formation tendencies of the optical isomers of
LE were evaluated by nanoESI -
MS using quadruply deuterated
LE (
H2N - Tyr -( 2,2 - d2 ) Gly -( 2,2 - d2 ) Gly - Phe - Leu - COOH ,
d4 - LE ) as an internal standard . -
pubmed
It was positive in vacuole with
acridine orange and
acid phosphatase stain . -
pubmed
Upon in vitro decidualization of endometrial stromal cells with
progesterone ,
PAPP - A levels in CM increased nearly 9 - fold without a concomitant change in
proMBP . -
pubmed
In contrast to the experiments with trophoblasts ,
IGF - II and the
IGF analogues ,
Leu(27 )
IGF - II , and Des ( 1 - 6 )
IGF - II , resulted in a dose - dependent decrease of
PAPP - A levels in decidualized endometrial stromal CM by 70 - 90 % , and a dose - dependent increase in
proMBP of 14- to 41 - fold . -
pubmed
Among the most detailed mediators studied are
corticotropin - releasing factor and
serotonin which , via the hypothalamic - pituitary - adrenal axis and the sympathetic and parasympathetic nervous system , stimulate
catecholamines and
cortisol and inhibit anabolic hormones ,
insulin ,
leptin , ghrelin , including
neuropeptide Y and other neuropeptides , among them the paracrine - acting cytokines . -
pubmed
Cadaverine inhibition of
porin plays a role in cell survival at acidic pH. -
pubmed
In order to understand the physiological significance of
cadaverine excretion and the inhibition of
porins , we isolated an
OmpC mutant that showed resistance to
spermine during growth and
polyamine - resistant
porin - mediated fluxes . -
pubmed
Here , we show that the addition of exogenous
cadaverine allows wild - type cells to survive a 30 - min exposure to pH 3.6 better than cells expressing the
cadaverine - insensitive
OmpC porin . -
pubmed
Competition experiments between
strains expressing either wild - type or mutant
OmpC showed that the lack of sensitivity of the
porin to
cadaverine confers a survival disadvantage to the mutant cells at reduced pH. -
pubmed
On the basis of these results , we propose that the inhibition of
porins by excreted
cadaverine represents a novel mechanism that provides bacterial cells with the ability to survive acid stress . -
pubmed
HHV-8 - gB possesses the
Arg - Gly - Asp ( RGD ) motif , the minimal peptide region of many proteins known to interact with subsets of host cell surface
integrins . -
pubmed
A truncated form of
gB without the transmembrane and
carboxyl domains ( gBdeltaTM ) and a gBdeltaTM mutant ( gBdeltaTM - RGA ) with a single
amino acid mutation ( RGD to RGA ) were expressed in a baculovirus system and purified . -
pubmed
In contrast , adhesion was not blocked by the preincubation of plate - bound
gBdeltaTM protein with heparin , suggesting that the adhesion is mediated by the RGD
amino acids of
gB , which is independent of the heparin - binding domain of
gB. -
pubmed
Adhesion induced by the
gBdeltaTM was blocked by
EDTA , thus suggesting the role of
integrins in the observed adhesions . -
pubmed
Inhibition of
FAK phosphorylation by
genistein blocked
gBdeltaTM - induced
FAK activation and cell adhesion . -
pubmed
These included measurement of
parathyroid hormone (
PTH ) or
vitamin D metabolites , or receipt of
calcitriol or
calcium - containing
phosphate binders ( CCPB ) prior to RRT . -
pubmed
RESULTS : Only 3.4 % of
CKD patients had their
PTH assessed prior to RRT , and 0.3 % had
vitamin D status measured . -
pubmed
We observed no interference from
anti - HBc antibody or blood components , but the assay was inhibited by very high concentrations ( & gt;1 microg / ml ; corresponding to 80 signal / cutoff ) of
HBeAg . -
pubmed
Solutes were separated on a
C18 column with a mobile phase of
methanol -
acetate buffer ( pH 4.0 ) ( 80:20 ) . -
pubmed
Expression profiling identified processes likely responsible for
18 - fluoro-2 - deoxy - glucose uptake and
tumor localization by positron emission tomography , and a role for
hypoxia - inducible factor-1 was suggested . -
pubmed
[ Current
ACTH and
cortisone therapy ] . -
pubmed
[
DESOXYGLUCOSYLTHYMINE AS AN INHIBITOR OF
PYRIMIDINE - NUCLEOSIDE - PHOSPHORYLASE FROM
ASCITES TUMOR CELLS ] . -
pubmed
[ ON THE EFFECT OF THE
ENZYME INHIBITOR
TRASYLOL IN THE THERAPY AND PROPHYLAXIS OF
ACUTE PANCREATITIS : REPORT BASED ON 893 CASES ] . -
pubmed
Recently , a family of
tyrosine kinase inhibitors , the
tyrphostins , has been successfully used in models of
endotoxemia ,
peritonitis , and
hypovolemic shock . -
pubmed
CONCLUSIONS :
Tyrphostin Ag 556 alters
spinal cord ischemia reperfusion injury by inhibiting
protein kinases . -
pubmed
ESI
MS analysis of
NAT1 treated with
Br - AAF showed that a single molecule of
2 - acetylaminofluorene had been incorporated . -
pubmed
Proteolysis with
pepsin followed by sequencing of adducted peptides by ESI
MS /
MS identified the modified residue as the catalytically essential
Cys-68 . -
pubmed
ESI Q -
TOF MS analysis of
NAT1 that had been treated with
bromoacetanilide resulted in identification of a monoadducted protein as the primary product and a diadducted protein as a minor product . -
pubmed
Pepsin digestion of
bromoacetanilide - inactivated
NAT1 and sequencing by ESI
MS /
MS identified
Cys-68 as the primary site of adduct formation . -
pubmed
Additional proteolysis of the
bromoacetanilide - treated
NAT1 led to the identification of a second modified peptide which was adducted at
Cys-44 . -
pubmed
Zinc depletion resulted in a decrease in the activity levels of the
CDP -
diacylglycerol pathway enzymes
phosphatidylserine synthase ,
phosphatidylserine decarboxylase ,
phosphatidylethanolamine methyltransferase , and
phospholipid methyltransferase . -
pubmed
In contrast , the activity of
phosphatidylinositol synthase was elevated in response to
zinc depletion . -
pubmed
For the
CHO1 - encoded
phosphatidylserine synthase , the reduction in activity in response to
zinc depletion was controlled at the level of transcription . -
pubmed
This regulation was mediated through the
UAS(INO ) element and by the transcription factors
Ino2p ,
Ino4p , and
Opi1p that are responsible for the
inositol - mediated regulation of
UAS(INO)-containing genes involved in phospholipid synthesis . -
pubmed
A
zrt1Delta zrt2Delta mutant ( defective in the plasma membrane zinc transporters
Zrt1p and
Zrt2p ) grown in the presence of
zinc exhibited a phospholipid composition similar to that of wild type cells depleted for
zinc . -
pubmed
Determination of
midazolam and its metabolite as a probe for
cytochrome P450 3A4 phenotype by liquid chromatography - mass spectrometry . -
pubmed
This study demonstrated the analysis of
midazolam and its metabolites by liquid chromatography - mass spectrometry ( LC -
MS ) with a sonic spray ionization ( SSI ) interface . -
pubmed
We also investigated the effect of
propofol on the metabolism of
midazolam using recombinant
CYP3A4 . -
pubmed
Propofol competitively inhibited the metabolism of
midazolam to
1 '- hydroxymidazolam by
CYP3A4 . -
pubmed
The
EGFR ,
EGFRvIII expression and
tyrosine phosphorylation ( Tyr - P ) levels were quantified by Western blotting . -
pubmed
Docetaxel plus
epirubicin as first - line chemotherapy in MBC ( KCSG 01 - 10 - 05 ): phase II trial and the predictive values of circulating
HER2 extracellular domain and
vascular endothelial growth factor . -
pubmed
We conducted a multicenter phase II study to evaluate the efficacy and tolerability of the
docetaxel plus
epirubicin combination chemotherapy as first - line treatment in
MBC and performed a prospective assessment of the predictive values of circulating
HER2 extracellular domain (
ECD ) and
vascular endothelial growth factor (
VEGF ) . -
pubmed
[ Synthesis of
purpure-18 - imide and study on its interaction with
bovine serum albumins by fluorescence ] . -
pubmed
The binding reaction between
purpure-18 - imide and
bovine serum albumins (
BSA ) in aqueous was studied by fluorescence and UV - Vis absorption spectra . -
pubmed
In aqueous ,
purpure-18 - imide strongly bound
BSA with molar ratio of 1:1 . -
pubmed
About 40 % of
hK11 is enzymatically active , whereas the
rest is inactivated by internal cleavage after
Arg156 ( Genbank accession no . -
pubmed
Purified
hK11 possesses
trypsin - like activity and cleaves synthetic peptides after
arginine but not
lysine residues . -
pubmed
The strongest inhibitor ,
APMSF , completely inhibited
hK11 activity at a concentration of 2.5 mmol / L. Aprotinin and an
hK11 - specific monoclonal antibody inhibited
hK11 activity up to 40 % . -
pubmed
Plasmin is a strong candidate for cleaving
hK11 at
Arg156 . -
pubmed
The
sulfide oxidase was purified to homogeneity and the molecular weight of the purified
enzyme was 43 kDa when estimated by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis . -
pubmed
The purified
sulfide oxidase can be effectively immobilized in
DEAE (
diethylaminoethyl)-cellulose matrix with a yield of 66 % . -
pubmed
The purified free
sulfide oxidase activity was completely inhibited by 1 mM
Co2 + and
Zn2 + and
sulfhydryl group reagents (
para - chloromercuribenzoic acid and
iodoacetic acid ) . -
pubmed
Empiric treatments include the use of
calcium channel blockers ,
steroids , induced
hypertension or blood pressure lowering , and rarely endovascular therapy . -
pubmed
Taking into account the structural requirements for TICT - type sensor molecules , a general synthetic route to derive pH and cation - responsive pretwisted donor
( D )- donor ( D ) biphenyls ( b ) equipped with donor receptors is developed and a first model compound containing a
mono aza-15 - crown-5 and a
DMA receptor is synthesized , see Scheme 1 . -
pubmed
Therefore , these results indicate that
glutathione degradation occurs within vacuoles or at the
tonoplast , rather than at the plasmalemma or in the apoplast . -
pubmed
Activity of
PXD101 , a
histone deacetylase inhibitor , in preclinical
ovarian cancer studies . -
pubmed
In the current investigation , we examined the activity of
PXD101 , a potent
histone deacetylase inhibitor , used alone or in combination with clinically relevant chemotherapeutics (
docetaxel ,
paclitaxel , and
carboplatin ) , in preclinical in vitro and in vivo models of
ovarian cancer . -
pubmed
In support of these findings ,
PXD101 was shown to increase the acetylation of
alpha - tubulin induced by
docetaxel and the phosphorylation of
H2AX induced by
carboplatin . -
pubmed
AIM : The aim of this study was to examine the potential effects of
COCs on women with
hypoactive sexual desire disorder (
HSDD ) . -
pubmed
A two - tailed t - test comparison was made between the two groups comparing free and total
testosterone and
sex hormone - binding globulin (
SHBG ) . -
pubmed
MAIN OUTCOME MEASURES : The main outcome measures are the differences between the two groups comparing free
testosterone , total
testosterone , and
SHBG . -
pubmed
The
SHBG was significantly higher in the group on
COCs compared with those who were not on
COCs . -
pubmed
Production of
1,25 ( OH )( 2 ) D ( 3 ) is catalysed by the enzyme
25 - hydroxyvitamin D ( 3 )- 1alpha - hydroxylase (
CYP27B1 ) . -
pubmed
Cells were treated with either
parathyroid hormone (
PTH ) ,
1,25 ( OH )( 2 ) D ( 3 ) ,
transforming growth factor - beta (
TGF - beta ) or
insulin - like growth factor-1 (
IGF-1 ) and
luciferase activity was measured 24h later . -
pubmed
The results showed that
1,25 ( OH )( 2 ) D ( 3 ) did not alter expression of the reporter construct , however treatment with
PTH ,
IGF-1 and
TGF - beta decreased expression by 18 , 53 and 58 % respectively . -
pubmed
The incretin effect was measured as the difference in
insulin secretion ( area under the curve [ AUC ] ) in response to an oral
glucose tolerance test ( OGTT ) and to an isoglycemic intravenous
glucose test . -
pubmed
One month after RY -
GBP ,
body weight decreased by 9.2 + /- 7.0 kg , oral
glucose - stimulated
GLP-1 ( AUC ) and
GIP peak levels increased significantly by 24.3 + /- 7.9 pmol x l(-1 ) x min(-1 ) ( P & lt ; 0.0001 ) and 131 + /- 85 pg / ml ( P = 0.007 ) , respectively . -
pubmed
Gliadin fibers have high strength ( 120 MPa ) and elongation ( 25 % ) compared to similar
collagen fibers that were crosslinked with
glutaraldehyde ( strength of about 44 MPa and elongation of 14 % ) . -
pubmed
18FDG - PET scan and
T1 enhanced MRI follow - up studies were performed between 3 and 5 months after treatment . -
pubmed
Intracellular pH homeostasis and
serotonin - induced pH changes in Calliphora salivary glands : the contribution of
V - ATPase and
carbonic anhydrase . -
pubmed
Blowfly salivary gland cells have a
vacuolar - type H (+)- ATPase (
V - ATPase ) in their apical membrane that energizes secretion of a
KCl - rich saliva upon stimulation with
serotonin (
5 - hydroxytryptamine , 5 -
HT ) . -
pubmed
We have used
BCECF to study microfluometrically whether
V - ATPase and
carbonic anhydrase (
CA ) are involved in intracellular pH ( pH(i ) ) regulation , and we have localized
CA activity by histochemistry . -
pubmed
We show : ( 1 ) mean pH(i ) in salivary gland cells is 7.5+/-0.3 pH units ( N=96 ) , higher than that expected from passive
H (+ ) distribution ; ( 2 ) low
5 - HT concentrations ( 0.3 - 3 nmol l(-1 ) ) induce a dose - dependent acidification of up to 0.2 pH units , with 5 - HT concentrations & gt;10 nmol l(-1 ) , causing monophasic or multiphasic pH changes ; ( 3 ) the acidifying effect of
5 - HT is mimicked by bath application of
cAMP ,
forskolin or
IBMX ; ( 4 ) salivary gland cells exhibit CA activity ; ( 5 ) CA inhibition with
acetazolamide and
V - ATPase inhibition with
concanamycin A lead to a slow acidification of steady - state pH(i ) ; ( 6 ) 5 -
HT stimuli in the presence of
acetazolamide induce an alkalinization that can be decreased by simultaneous application of the
V - ATPase inhibitor
concanamycin A ; ( 7 )
concanamycin A removes alkali - going components from multiphasic
5 - HT - induced pH changes ; ( 8)
NHE activity and a
Cl (-)-dependent process are involved in generating
5 - HT - induced pH changes ; ( 9 ) the salivary glands probably contain a
Na (+)- driven amino acid transporter . -
pubmed
We conclude that
V - ATPase and CA contribute to steady - state pH(i ) regulation and 5 - HT - induced outward
H (+ ) pumping does not cause an alkalinization of pH(i ) because of cytosolic
H (+ ) accumulation attributable to stimulated cellular respiration and AE activity , masking the alkalizing effect of
V - ATPase - mediated acid extrusion . -
pubmed
Arginase I , an abundant
manganese (
Mn)-binding protein in the liver , requires
Mn as an essential element to exhibit maximal enzyme activity . -
pubmed
In the present study , we examined the effect of
MeHg on hepatic
arginase I in vivo and in vitro . -
pubmed
With purified
arginase I , we found that interaction of
MeHg with
arginase I caused the aggregation of
arginase I as evaluated by centrifugation and subsequent precipitation , and then the reduction of catalytic activity . -
pubmed
Experiments with
organomercury column confirmed that
arginase I has reactive
thiols that are covalently bound to
organomercury . -
pubmed
While
MeHg inhibited
arginase I activity ,
Mn ions were released from this enzyme . -
pubmed
These results suggest that
MeHg - mediated suppression of hepatic
arginase I activity in vivo is , at least in part , attributable to covalent modification of
MeHg or substantial leakage of
Mn ions from the active site . -
pubmed
Chondroitin
sulfate (
CS )
dermatan sulfate (
DS ) , and
CS /
DS hybrid chains are biologically active like
heparan sulfate , and structurally the most complex species of the glycosaminoglycan family along with
heparan sulfate . -
pubmed
They function as regulators of functional proteins such as growth factors ,
cytokines ,
chemokines , adhesion molecules , and lipoproteins through interactions with the ligands of these proteins via specific
saccharide domains . -
pubmed
Recent microsequencing of
CS /
DS oligosaccharides that bind growth factors , such as
pleiotrophin , and various monoclonal antibodies against
CS /
DS , have revealed a considerable number of unique
oligosaccharide sequences . -
pubmed
MATERIALS & amp ; METHODS : Two antiprostate - specific antigen scFvs mutants -- one with an 11 - residue
c - myc ( referred as scFvB80 -
M1 ) and the other with a
lysine - enriched
His 6 - tagging peptide attached to their C - termini ( referred as scFvB80 - M2)--were prepared . -
pubmed
These two
scFv mutants were conjugated directly with
CdSe /
ZnS Qdots and their binding activities were measured and compared . -
pubmed
RESULTS & amp ; DISCUSSION : Both
scFv mutants can be conjugated covalently with
CdSe /
ZnS Qdots ; however , the resulting conjugates exhibit significantly different affinities in the prostate - specific antigen fluoroimmunoassays -- the binding activity of scFvB80 - M2 / Qdots is equivalent of that of free scFvB80 and four times of that of scFvB80 -
M1 / Qdots . -
pubmed
Upon strand separation by a
helicase and
ATP , the beacon strand forms an intramolecular hairpin that brings the tethered fluorescent and quencher molecules into juxtaposition , quenching fluorescence . -
pubmed
Calcium binding of rE -
Syt2 triggered structural rearrangements and initiated reversible multimerization of the protein in vitro . -
pubmed
IV mannitol ) , the appropriate treatment for
CFP remains unclear to many physicians . -
pubmed
It is proposed to study the effect of
ALA on
TD induced by
HAL and to correlate it with oxidative stress by studying total antioxidant status and lipid peroxidation (
LP ) . -
pubmed
ALA was suspended in 0.2 % carboxy methyl cellulose at a dose of 25 , 50 and 100 mg / kg and was administered orally by oral gavage 1 h before
HAL on 21st day of treatment . -
pubmed
Fitting a single - phase model to the
post - exercise changes in heart rate and
oxygen uptake . -
pubmed
Half - time for VO2 negatively correlated with square root of exercise duration ( within - subject r = -0.629 , p & lt ; 0.001 ) , the total
post - exercise
oxygen uptake till t(1/2 ) was thus constant irrespectively of exercise intensity . -
pubmed
The renal distal convoluted tubule (
DCT ) has an essential role in maintaining systemic
magnesium (
Mg ( 2 + ) ) concentration . -
pubmed
The
DCT is the final determinant of plasma
Mg ( 2 + ) levels , as the more distal nephron segments are largely impermeable to
Mg ( 2 + ) . -
pubmed
A large fraction of this resides in the DCT , namely , ( i ) the
transient receptor potential channel melastatin subtype 6 (
TRPM6 ) , a divalent cation - permeable channel located at the luminal membrane of the DCT , facilitates
Mg ( 2 + ) entry from the pro - urine into the cell ; ( ii ) the
epidermal growth factor is a novel hormone regulating active
Mg ( 2 + ) transport through
TRPM6 ; ( iii ) the voltage - gated
K (+ ) channel ,
Kv1.1 , establishes a favorable luminal membrane potential for
TRPM6 - mediated
Mg ( 2 + ) transport ; ( iv ) the
Na (+)/ K (+)- ATPase gamma - subunit (
gamma - Na (+)/ K (+)- ATPase ) was identified as mutated protein in a family with isolated dominant
hypomagnesemia . -
pubmed
The aim of the present in vitro investigation was to elucidate the impact of AB0 blood group - dependent
vWF concentrations on
eptifibatide and
abciximab mediated inhibition of
GPIIb / IIIa function . -
pubmed
If measured with the
collagen /
ADP cartridge , blood group 0 was associated with a prolonged mean baseline closure time in comparison with blood group A ( 94.3 + /- 14.6 s vs. 74.6 + /- 9.9 s , p = 0.007 ) which was paralleled by reduced concentrations of
vWF and
factor VIII . -
pubmed
The effects of
triphosphopyridine nucleotide and of
adenosine triphosphate on
pigeon liver oxalacetic carboxylase . -
pubmed
Addition of induction therapy offsets the hazard of marked
calcineurin minimization among renal transplant recipients treated with
sirolimus . -
pubmed
Association between
FokI polymorphism in
vitamin D receptor gene and susceptibility to
spinal tuberculosis in Chinese Han population . -
pubmed
BACKGROUND AND AIMS : Studies have shown that the
vitamin D receptor (
VDR ) gene polymorphisms may be associated with
pulmonary tuberculosis (
TB ) and play important roles in the regulation of
calcium in a variety of tissues including bone . -
pubmed
Adenylosuccinate lyase (
ADSL ) is a bifunctional enzyme acting in de novo
purine synthesis and
purine nucleotide recycling . -
pubmed
Nucleotide sequence analysis of the PCR amplicons followed by GenBank nucleotide BLASTn analysis revealed five novel DNA sequences ; translated
amino acid BLASTx analysis confirmed sequence similarity to class 1b major structural proteins encoded by
csnA ,
fotA , and
fasA. -
pubmed
Accuracy of parathyroid imaging : a comparison of planar scintigraphy , SPECT , SPECT -
CT , and
C-11 methionine PET for the detection of parathyroid
adenomas and
glandular hyperplasia . -
pubmed
PURPOSE : To compare the accuracy of planar scintigraphy , single photon emission computed tomography ( SPECT ) , SPECT -
CT , and positron emission tomography ( PET ) with
C-11 methionine for the pre - operative detection of parathyroid
adenomas . -
pubmed
In the case of negative scintigraphic findings and proven
hyperparathyroidism , additional
C-11 methionine PET or PET -
CT is recommended . -
pubmed
The scope of this study is to modify a Surface Plasmon Resonance (
SPR ) sensor slide with
isophthalic acid to evaluate the possible application on the detection of
copper ( II ) ions in aqueous media by total internal reflection ellipsometry . -
pubmed
Isophthalic acid monolayer modified gold slides were used for in situ detection of aqueous
Cu ( 2 + ) solution with the
SPR enhanced total internal reflection ellipsometry ( SPRe - TIRE ) technique . -
pubmed
Gibberellins negatively regulate low temperature - induced
anthocyanin accumulation in a
HY5 /
HYH - dependent manner . -
pubmed
Recently , two
bZIP transcription factors LONG HYPOCOTYL 5 (
HY5 ) and
HOMOLOG OF HY5 (
HYH ) were identified to play an important role in the process of low temperature - induced
anthocyanin accumulation . -
pubmed
However , the mechanism by which
HY5 /
HYH regulates
anthocyanin accumulation under low temperature still remains unclear . -
pubmed
Moreover ,
hy5hyh mutant was insensitive to
PAC in enhancing
anthocyanin accumulation under low temperature . -
pubmed
From these data we propose that , together with
HY5 /
HYH ,
GA signaling may play an important role during low temperature - induced
anthocyanin accumulation . -
pubmed
At least two pesticides were detected at every site including insecticides , fungicides , herbicides , and the Reef Plan 's ( 2009 ) five priority photosystem
II ( PSII ) herbicides (
diuron ,
atrazine ,
hexazinone ,
tebuthiuron and
ametryn ) . -
pubmed
On morphology and strain field of
Ge /
Si(001 ) Islands according to
TEM phase imaging method . -
pubmed
Cd treatments led to a decline in
mycorrhizal infection ( MI ) , nodule number and dry weights which was accompanied by reductions in
leghemoglobin content ,
nitrogenase activity , organic acid contents . -
pubmed
The lard - rich diet increased liver
CL synthase gene expression while the
fish oil - rich diet increased the ( n-3 ) polyunsaturated
fatty acids content in CL . -
pubmed
These include , amongst others , the
Rho GTPase ,
focal adhesion kinase ,
cyclooxygenase-2 ,
beta - catenin signalling and
calcium signalling . -
pubmed
A total of 164 bacteria - specific probes were designed from
16S rRNA gene sequences to target 12 bacteria species , including
lactic acid bacteria and selected food pathogens . -
pubmed
In the present study , we administered
CO to the same model of
ALI only after resuscitation and examined whether it exerted a therapeutic effect without adverse events on HSR - induced
ALI , since treatment of animals with
CO prior to
HSR did not prevent
lung injury . -
pubmed
This was determined from an observed increase in the number of cells positive for
transferase - mediated
dUTP -
fluorescein isothiocyanate (
FITC ) , nick - end labeling staining and activated
caspase-3 . -
pubmed
The protective effects of
CO against
lung injury were notably associated with an increase in the protein expression level of
peroxisome proliferator - activated receptor ( PPAR )- gamma , an anti - inflammatory transcriptional regulator in the lung . -
pubmed
Moreover ,
CO inhalation did not affect the hemodynamic status or tissue oxygenation during
HSR . -
pubmed
These findings suggest that inhalation of
CO at a low concentration exerts a potent therapeutic effect against
HSR - induced
ALI and attenuates the inflammatory cascade by increasing
PPAR - gamma protein expression . -
pubmed
The affinity tag consists of an N - terminal 6xHis - tag , a
choline binding domain followed by a proteolytic site specific for the TEV (
tobacco etch virus ) endopeptidase . -
pubmed
Based on the
choline - binding
His - tag combination the new 16.5 kDa tag was named
CHiC. -
pubmed
The
STAT5 Inhibitor
Pimozide Displays Efficacy in Models of
Acute Myelogenous Leukemia Driven by
FLT3 Mutations . -
pubmed
We have identified the psychotropic drug
pimozide as an effective inhibitor of
STAT5 function . -
pubmed
Pimozide inhibits the
tyrosine phosphorylation of
STAT5 , leading to the
death of
AML cells through the induction of apoptosis . -
pubmed
Pimozide shows a combinatorial effect with the
tyrosine kinase inhibitors
midostaurin (
PKC412 ) and
sunitinib in the inhibition of
STAT5 tyrosine phosphorylation and the induction of apoptosis . -
pubmed
The
HSP90 inhibitor
NVP - AUY922 - AG inhibits the
PI3 K and
IKK signalling pathways and synergizes with
cytarabine in
acute myeloid leukaemia cells . -
pubmed
Here , we investigated
HSP90 as a molecular target for
acute myeloid leukaemia (
AML ) using the novel
HSP90 inhibitor
NVP - AUY922 - AG . -
pubmed
NVP - AUY922 - AG induced increases in
HSP70 expression and depletion of total
AKT ,
IKKalpha and
IKKbeta in cell lines and primary blasts . -
pubmed
This study shows that the novel
HSP90 inhibitor
NVP - AUY922 - AG has significant single agent activity in
AML cells and is synergistic with
Ara - C. -
pubmed
A sensitive analytical method was developed for the simultaneous determination of
glyphosate ,
glufosinate and
aminomethylphosphonic acid by capillary electrophoresis with laser - induced fluorescence detection ( CE -
LIF ) . -
pubmed
Every quarter , mean levels and prevalence of conformity were collected for the following indicators :
anemia , dialysis dose , serum
calcium and
phosphorus ,
PTH ,
25OH - vitamin D ,
albumin , serum
bicarbonate ,
LDL -
cholesterol , serum
beta2 - microglobulin , systolic and diastolic blood pressure , intradialytic
hypotension and vascular access . -
pubmed
An improvement in the percentage of conformity was observed for
hemoglobin , dialysis dose ,
phosphates ,
PTH , serum
bicarbonate and
beta2 - microglobulin in the global population . -
pubmed
Finally , we suggest that the definition and targets of some indicators , especially
hypertension and
LDL -
cholesterol , be reviewed , since evidence of their association with mortality is not demonstrated . -
pubmed
Reactions at room temperature in water -
ethanol mixtures give rise to hydrated kinetic products , including two new crystal structures ,
[ Li2 ( D , L - tartrate )( H2O ) 2 ] n in
P1 and
[ Li2 ( meso - tartrate )( H2O )( 0.5 )] n in C222(1 ) , whose structures are contrasted with known
anhydrous products . -
pubmed
METHODS : Planar and SPECT /
CT scans of a water phantom containing a 70 ml bottle filled with
lutetium-177 (
Lu ) were acquired over 60 days . -
pubmed
RESULTS : For count rates that were about two - fold higher than typically seen in
post - therapy
Lu scans , the maximum DTCF reached a level of about 17 % . -
pubmed
The properties of mutants affecting the biosynthesis of
leucine and its regulation suggest that the expression of
LEU1 and
LEU2 ( structural genes encoding
isopropylmalate isomerase and
beta - isopropylmalate dehydrogenase , respectively ) is controlled by a complex of a -
isopropylmalate and a regulatory element ( the
LEU3 gene product ) . -
pubmed
Initial investigations revealed
atrial fibrillation (
AF ) and
pericardial effusion , further investigations unraveled
primary hypothyroidism (
thyroid stimulating hormone ) of 34.7 muIU / ml and total
thyroxine ( T4 ) of 5.57 mug / dl ) . -
pubmed
The incidence of four - year MACE was significantly higher in BA ( 75.2 % ) than in
DES implantation ( 45.8 % , p<0.001 ) , mainly because of the lower TLR rate in
DES implantation ( 60.5 % vs. 27.6 % , p<0.001 ) . -
pubmed
Radiosynthesis of
carbon-11 and
fluorine-18 labelled radiotracers to image the
ionotropic and metabotropic glutamate receptors . -
pubmed
l - Glutamate is the major neurotransmitter in the central nervous system and activates both
ionotropic and metabotropic receptors . -
pubmed
A tetradecapeptide
somatostatin dicarba - analog : Synthesis , structural impact and biological activity . -
pubmed
Remarkably , this
alkene - bridged
somatostatin analog is highly selective against
somatostatin receptors 1 and 5 , suggesting that conformational rigidity is not required for the efficient interaction of
somatostatin analogues with these two receptors . -
pubmed
Washed leaves were crushed in a meat - mill in an isotonic or slightly hypertonic medium containing
sucrose , EDTA ,
MgSO4 ,
bovine serum albumin ,
mercaptoethanol ,
KH2PO4 , and
HEPES as a buffer substance . -
pubmed
After resuspension in a washing medium , the pellet was centrifuged in a swing - out rotor for 30 min at 35000xg on a density gradient generated from a
silica sol ( " Ludox
HS 40 " ) . -
pubmed
The respiration rate was 29.5 muatoms O / mg
protein - N / h with
alpha - ketoglutarate as a substrate . -
pubmed
The
arsenic ambient water quality criteria ( AWQC ) , based on the assumption of a linear mode of action for
skin cancer risk , results in an allowable limit of 0.018ppb in ambient waters ; the drinking water Maximum Contaminant Level (
MCL ) was determined using a similar linear approach . -
pubmed
tuberculosis
sigma ( K ) in complex with the cytosolic domain of
RskA (
RskAcyto ) revealed a
disulfide bridge in the -35 promoter - interaction region of
sigma ( K ) . -
pubmed
Biochemical experiments reveal that the redox potential of the
disulfide - forming
cysteines in
sigma ( K ) is consistent with its role as a sensor . -
pubmed
The
disulfide bond in
sigma ( K ) influences the stability of the
sigma ( K )- RskAcyto complex but does not interfere with
sigma ( K )-promoter DNA interactions . -
pubmed
Photoluminescence (
PL ) measurements of
ZnO :
Eu3 + showed a strong red luminescence emission . -
pubmed
The intensity of
Eu3 + PL decreased with the increasing
Eu3 + concentration and the concentration quenching mechanism was presented based on non - radiative recombination processes in
ZnO and the energy back - transfer from the excited state of
Eu3 + to the
ZnO host . -
pubmed
Association between single
nucleotide polymorphisms of
asporin (
ASPN ) and
BMP5 with the risk of
knee osteoarthritis in a Chinese Han population . -
pubmed
Fe3O4 peroxidase mimetics as a general strategy for the fluorescent detection of
H2O2 - involved systems . -
pubmed
We developed a general
H2O2 - involved fluorescence system using
Fe3O4 magnetic microspheres as
peroxidase mimetics and
benzoic acid ( BA ) as indicator . -
pubmed
Glucose oxidase hydrolyzes
glucose , in the presence of
oxygen , to
H2O2 followed by the activation of
Fe3O4 MMs , resulting in the catalyzed oxidation of
benzoic acid . -
pubmed
Reducing
AgNO3 with
NaBH4 in
CNF suspensions produced smaller but more uniform
Ag nanospheres ( AgNSs ) with increasing
Ag(+)/CNF ratios . -
pubmed
CNF bound AgNSs were facilely transformed to AgNPs by etching with
H2O2 , supporting the capping and shape - regulating capability of CNFs . -
pubmed
The AgNPs transformed from
CNF bound AgNSs are similar to those synthesized directly , but more stable against
H2O2 . -
pubmed
The
CNF surface bound AgNPs were easily fabricated into freestanding
CNF / AgNPs films that showed excellent surface enhanced Raman scattering of
Rhodamine 6 G with analytical enhancement factor of 5 x 10(3 ) in contrast to none from the
CNF /
AgNSs film . -
pubmed
Tetraspanin
CD9 is considered to be a serious candidate
molecule participating in these events . -
pubmed
Since
caffeine is a mixed nonspecific
A ( 1 ) and A ( 2A ) adenosine receptor antagonist , we measured also the influence of antagonists and agonists of these receptors on SWD expression . -
pubmed
GAERS were equipped with four cortical electrodes over the frontoparietal cortex and the cumulated duration and number of SWDs were recorded for 120 min after the injection of increasing doses of
caffeine , specific antagonists and agonists of
A ( 1 ) and
A ( 2A ) adenosine receptors . -
pubmed
These data show that the acute exposure to low doses of
caffeine , or A(1 ) and
A ( 2A ) adenosine receptor antagonists reduces SWD expression in GAERS , independently from any effect on motor activity . -
pubmed
Two extrinsic fluorescent probes ,
3 -( dimethylamino )- 8,9,10,11 - tetrahydro-7H - cyclohepta [ a ] naphthalen-7 - one ( 1 ) and
7 -( dimethylamino )- 2,3 - dihydrophenanthren-4 ( 1H )- one ( 2 ) , are used to probe the unfolding of
human serum albumin by
sodium dodecyl sulfate (
SDS ) . -
pubmed
Each subsequent equivalent of
SDS gives rise to a dramatic increase in polarity until
HSA is saturated with seven molecules of
SDS at the end of the specific binding domain . -
pubmed
This result is consistent with greater exposure of the
carbonyl group in 2 , but not the bulk of 2 , to the aqueous solvent in site II of the
SDS - saturated
HSA complex . -
pubmed
To validate these observations , the expression of
Keap1 protein was restored in
arsenite - transformed cells by treatment with a
DNA methyltransferase inhibitor ,
5 - aza-2 '- deoxycytidine (
5 - Aza - dC ) , and protein levels of
Nrf2 and colony formation were then determined after these treatments . -
pubmed
Results showed that enhancement of
Keap1 expression by
5 - Aza - dC significantly reduced
Nrf2 and its target antioxidant enzyme levels , and that in turn suppressed cell proliferation and colony formation of the transformed cells . -
pubmed
The inhibition of
noradrenaline re - uptake by
propiverine mainly occurs at the urethral level and partially in the central nervous system , and might stimulate the smooth muscle of the bladder neck and proximal urethra through
alpha1 - adrenergic receptors , as well as stimulating the striated muscle of the urethra and pelvic floor by activation of spinal motoneurons . -
pubmed
The in - vitro antioxidant effect of
ECP was investigated using
1 - diphenyl-2 - picrylhydrazyl , reducing power ,
beta - carotene bleaching and
thiobarbituric acid reactive substances assays . -
pubmed
BACKGROUND / OBJECTIVE : The mechanistic target of
rapamycin (
mTOR ) is a
serine -
threonine kinase that functions into distinct protein complexes (
mTORC1 and
mTORC2 ) that regulate energy homeostasis . -
pubmed
RESULTS : We first report that systemic ( brain and periphery ) overexpression of
DEPTOR prevents high - fat diet - induced
obesity , improves
glucose metabolism and protects against
hepatic steatosis . -
pubmed
Ten - eleven
translocation enzyme 2 (
TET2 ) protein , one member of TET family , could rely on
alpha - ketoglutarate (
alpha - KG ) as cosubstrate to exhibit catalytic activity of DNA demethylation . -
pubmed
Further analysis showed that
alpha - KG concentration had a positive correlation with both
haemoglobin A1c (
HbA1C ) and 2 h postprandial blood
glucose ( PBG ) ( r = 0 393 , P = 0 005 ; r = 0 320 , P = 0 025 , respectively ) . -
pubmed
BACKGROUND : Aerobic interval training ( IT ) seems to be superior to continuous training (
CT ) in improving exercise capacity ( peak
oxygen uptake ( VO2 ) ) in
coronary artery disease (
CAD ) patients in some , but not in all studies . -
pubmed
Higher
lactate levels were reached after IT sessions ( ITw 5.42 + - 1.42 mmol / l , ITc 5.05 + - 1.38 mmol / l ) compared to
CT sessions ( CTw 2.45 + - 1.04 mmol / l , CTc 3.41 + - 1.44 mmol / l ) ( p & lt ; 0.01 ) . -
pubmed
Microbial communities and
arsenic biogeochemistry at the outflow of an alkaline
sulfide - rich hot
spring . -
pubmed
Alkaline sulfide - rich hot
springs provide a unique environment for microbial community and
arsenic (
As ) biogeochemistry . -
pubmed
In this study , a representative
alkaline sulfide - rich hot
spring , Zimeiquan in the Tengchong geothermal area , was chosen to study
arsenic geochemistry and microbial community using Illumina MiSeq sequencing . -
pubmed
High ratios of
As ( V )/ AsSum ( total combined
arsenate and
arsenite concentrations ) ( 0.59 - 0.78 ) , coupled with high
sulfide ( up to 5.87 mg / L ) , were present in the hot
spring 's pools , which suggested
As ( III ) oxidation occurred . -
pubmed
This study improves our understanding of microbially - mediated
As transformation in
alkaline sulfide - rich hot
springs . -
pubmed
In this study , we used
glioma cell lines and
H2O2 to investigate the role of
JNK in
glioma cell parthanatos induced by oxidative stress . -
pubmed
We found that exposure to
H2O2 not only induced intracellular accumulation of ROS but also resulted in
glioma cell
death in a concentration- and incubation time - dependent manner , which was accompanied with cytoplasmic formation of
PAR polymer , expressional upregulation of
PARP-1 , mitochondrial depolarization , and
AIF translocation to nucleus . -
pubmed
Moreover , the phosphorylated level of
JNK increased markedly with the extension of
H2O2 exposure time . -
pubmed
Either attenuation of intracellular ROS with antioxidant
NAC or inhibition of
JNK phosphorylation with
SP600125 or
JNK siRNA could significantly prevent
H2O2 - induced parthanatos in
glioma cells . -
pubmed
Additionally , inhibition of
JNK with
SP600125 alleviated intracellular accumulation of ROS and attenuated mitochondrial generation of
superoxide . -
pubmed
Light - induced
vasopressin release enhances the responses of
SCN neurons to light . -
pubmed
Many
SCN neurons express
vasopressin , and it has been assumed that the role of
vasopressin in the
SCN reflects the activity of these cells . -
pubmed
Here we show that
vasopressin is also expressed in many retinal cells that project to the
SCN . -
pubmed
Light - evoked
vasopressin release contributes to the responses of
SCN neurons to light , and enhances expression of the immediate early gene c - fos in the
SCN , which is involved in photic entrainment of circadian rhythms . -
pubmed
The proposed model is applied to two motivating studies of
amyotrophic lateral sclerosis : a clinical trial of
ceftriaxone and the Pooled Resource Open - Access
ALS Clinical Trials database . -
pubmed
MATERIAL AND METHODS : Type 4 commercially pure
titanium disks and 5 porcelain dentin shades (
A2 ,
A3 , B2 ,
C2 , and
D3 ) were used . -
pubmed
CONCLUSIONS : The minimum ceramic thickness required to mask the underlying
titanium color is 0.5 mm for all shades except
D3 , which only needs 0.4 mm . -
pubmed
Astragaloside IV protects against podocyte
injury via
SERCA2 - dependent
ER stress reduction and
AMPKalpha - regulated autophagy induction in
streptozotocin - induced
diabetic nephropathy . -
pubmed
Docosahexaenoic acid enrichment in
NAFLD is associated with improvements in hepatic metabolism and hepatic
insulin sensitivity : a pilot study . -
pubmed
Bicarbonate Recycling by
HIF-1 - Dependent
Carbonic Anhydrase Isoforms 9 and 12 Is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells . -
pubmed
We tested the hypothesis that a network comprising
HIF-1alpha ,
carbonic anhydrase ( CA ) 9 and 12 isoforms , and
sodium - coupled bicarbonate cotransporters (
NBCs ) buffer intracellular pH through coordinated
bicarbonate recycling . -
pubmed
Contrary to the current understanding of
NP cell metabolism , analysis of metabolic - flux data from Seahorse XF analyzer showed that
CO2 hydration contributes a significant source of extracellular proton production in
NP cells , with a smaller input from glycolysis . -
pubmed
Because enzymatic hydration of
CO2 is catalyzed by plasma membrane - associated CAs we measured their expression and function in
NP tissue . -
pubmed
As expected , inhibition of CA activity decreased extracellular acidification rate independent of changes in
HIF activity or
lactate / H+ efflux . -
pubmed
These results suggested that extracellular
bicarbonate generated by
CA9 / 12 is recycled to buffer cytosolic pH fluctuations . -
pubmed
Taken together , our studies show for the first time that
bicarbonate buffering through the
HIF-1alpha - CA axis is critical for
NP cell survival in the
hypoxic niche of the intervertebral disc . -
pubmed
Simulations using PK / PD models showed that
CYP2C9 genotype is the factor that affects the PDs of
meloxicam . -
pubmed
Curcumin suppresses
MUC5AC production via interfering with the
EGFR signaling pathway . -
pubmed
Curcumin was able to counteract the
endothelial growth factor (
EGF)-stimulated mRNA and protein expression of
MUC5AC . -
pubmed
In addition ,
curcumin treatment prevented
EGF - induced phosphorylation of
EGF receptor (
EGFR ) as well as the downstream
AKT and
signal transducer and activator of transcription 3 (
STAT3 ) , while inhibition of
PI3 K and
STAT3 signaling significantly attenuated the expression of
MUC5AC that was induced by
EGF . -
pubmed
Furthermore ,
EGF - induced increases in the levels of phosphorylated
STAT3 in the nuclear fraction were inhibited by
curcumin and
PI3 K inhibitors . -
pubmed
In addition , treatment with
curcumin significantly decreased
MUC5AC and
EGFR expression in a time - dependent manner under basal conditions . -
pubmed
Bayesian statistics identified 12 metabolites associated with clinical characteristics of postprandial vascular endothelial function , such as flow - mediated dilation (
FMD ) , postprandial plasma markers of oxidative stress and
NO status . -
pubmed
Serum blood
urea nitrogen ,
creatinine , and urinary and
serum kidney injury molecule-1 KIM-1 levels were measured . -
pubmed
Hemoglobin (
Hb ) target levels can be achieved through more frequent intravenous (
IV )
iron use with lower
ESA dose , or with less
iron dosing but higher
ESA . -
pubmed
ESA therapy to correct
anemia may result in severe arterial and venous
thrombotic complications and the evidence base evaluating hard clinical outcomes related to the use of
IV iron is sparse . -
pubmed
In Poland the use and dose of
IV iron was 35 - 72 % higher than that in Portugal ( p & lt ; 0.001 ) during three consecutive months ; use and dose of
ESA was 61 % higher in Portugal ( 5034 vs 3133 IU ( adjusted)/week , p & lt ; 0.001 ) . -
pubmed
CONCLUSIONS : Administration of high doses of
IV iron in routine clinical HD practice may not be associated with considerable harm . -
pubmed
Correction to : Unexpected Benefit from
Alpelisib and
Fulvestrant in a Woman with Highly Pre - treated
ER - Positive ,
HER2 - Negative
PIK3CA Mutant Metastatic
Breast Cancer . -
pubmed
Silver Nanoparticle - Induced Phosphorylation of
Histone H3 at
Serine 10 Involves
MAPK Pathways . -
pubmed
The phosphorylation of
histone H3 at
serine 10 ( p - H3S10 ) has been shown to be closely correlated with mitotic chromosome condensation . -
pubmed
We previously reported that intracellular
silver nanoparticles ( AgNPs ) release
Ag ions that alter
actin filament dynamics , leading to the activation of
Aurora kinases and the formation of
p - H3S10 through a mechanism clearly different from that occurring during mitosis . -
pubmed
We observed different effects of
AgNO3 (
Ag+ ) and AgNPs on cells , and only the
JNK inhibitor suppressed the temporary
AgNO3 - induced formation of p - H3S10 . -
pubmed
The photochemistry of
anthracene , a new class of photoresist for direct laser writing , is used to enable visible - light - gated control over the mechanical properties of 3D microstructures
post - manufacturing . -
pubmed
Applying correct WST class prediction from RFA modelling , new reference intervals were calculated for
activin B and associated pathology markers , where 24 - h urinary
creatinine clearance , serum
urea and serum
activin B showed the best potential as diagnostic markers . -
pubmed
HuR regulates
phospholamban expression in
isoproterenol - induced cardiac remodeling . -
pubmed
OBJECTIVE : The elevated expression of
phospholamban (
PLB ) has been observed in
heart failure and cardiac remodeling , inhibiting the affinity of
Ca2 + pump to
Ca2 + thereby impairing heart relaxation . -
pubmed
However , deletion of
HuR in cardiomyocytes mitigated the effect of
ISO in inducing
PLB expression and reducing
beta1 - AR expression , in turn aggravating
ISO - induced
myocardial hypertrophy and
cardiac fibrosis . -
pubmed
The
HuR -
PLB and
HuR - beta1 -
AR regulatory processes impact on
ISO - induced cardiac remodeling . -
pubmed
TRANSLATIONAL PERSPECTIVE :
PLB , the reversible inhibitor of
SERCA2A , is of critical importance for the
SR Ca2 + sequestration thereby the excitation - contraction coupling of myocytes and myocardial remodeling . -
pubmed
The activity of
PLB could be inhibited by
beta1 - AR -
cAMP -
PKA pathway through
PKA - mediated protein phosphorylation . -
pubmed
The escape latency ( EL ) ;
cerebral infarction area ratio ; positive apoptosis ; neuron pathological changes ; auto - phagosome numbers ; inflammatory factor contents ; mRNA and protein expressions of
Beclin-1 ,
Caspase-3 and
LC3II / I ; and the phosphorylation level of
JNK were decreased , while the times across platform and the times stayed in the quadrant of the original platform were increased after
dexmedetomidine treatment . -
pubmed
Decreased H3K9 acetylation level of
LXRalpha mediated
dexamethasone - induced
placental cholesterol transport dysfunction . -
pubmed
Results showed that
dexamethasone increased maternal serum total
cholesterol (
TC ) ,
high - density lipoprotein -
cholesterol (
HDL - C ) ,
low - density lipoprotein -
cholesterol (
LDL - C ) levels , as well as placental
cholesterol synthesis and TC concentration , while reduced fetal
birth weight , and serum
TC ,
HDL - C and
LDL - C levels . -
pubmed
Meanwhile , the expression of placental
cholesterol transporters , including
low - density lipoprotein receptor (
LDLR ) ,
scavenger receptor class B type I (
SR - B1 ) and
ATP - binding cassette transporter A1 and G1 (
ABCA1 and
ABCG1 ) were decreased by
dexamethasone . -
pubmed
Dexamethasone ( 2500 nM ) induced
GR translocation into nucleus and recruited
HDAC3 . -
pubmed
Furthermore ,
LXRalpha agonist and
GR inhibitor reversed respectively
dexamethasone - induced the expression inhibitions of
cholesterol transporter and
LXRalpha , and
HDAC3 siRNA reversed the H3K9ac level of
LXRalpha promoter and its expression . -
pubmed
Together ,
dexamethasone impaired placental
cholesterol transport and eventually decreased fetal
cholesterol levels , which is related to the down - regulation of
LXRalpha mediated by
GR /
HDAC3 / H3K9ac signaling . -
pubmed
Biochemical assays of
UGT76C family revealed that
UGT76C5 ( encoded by
At5g05890 , previously identified as
NaNGT ) was a multiple functional
nicotinate N - glycosyltransferase , with high preference to
UDP -
xylose and
UDP -
arabinose . -
pubmed
We dissect the following three key molecular events that occur as
Rad4 /
XPC tries to recognize and bind to DNA lesions / mismatches : ( a ) the association of
Rad4 /
XPC with the damaged / mismatched DNA , ( b ) the insertion of a lesion - sensing beta - hairpin of
Rad4 /
XPC into the damage / mismatch site and ( c ) the flipping of a pair of
nucleotide bases at the damage / mismatch site . -
pubmed
LC -
MS /
MS revealed that
PDD is associated with the
5 - methylaminomethyl-2 - thiouridine modification of chloroplast
tRNA . -
pubmed
Because of the many correlations between
oxytocin binding and bioactivity , it appears that
oxytocin binding sites on the plasma membrane of target cells constitute the recognition part of
oxytocin receptors . -
pubmed
The effects lasted for 24 h. Intraamniotic injection of 10 mg
oPRL prevented or reversed a doubling of water and
electrolyte content of the fetal extracellular fluid ( ECF ) volume in the
face of hypertonic amniotic fluid . -
pubmed
Radioactively - labeled
neurophysin bound to
polymethylmethacrylic particles was similarly infected into the animals . -
pubmed
Immunoperoxidase histochemical techniques were applied to
formalin - fixed kidney slices and demonstrated the presence of
neurophysin - like material localized in the cells of proximal tubules of the cortex and medulla . -
pubmed
The reference nootropic drug
piracetam given in a dose 200 mg / g for 9 days after surgery promoted
CAAR recovery . -
pubmed
Another peptide analogue of
piracetam -- pyroglutamyl - asparagine amide (
II ) showed no promoting effect on
CAAR recovery after frontal lobectomy . -
pubmed
Reaction of
dioxygen with
cytochrome c oxidase reduced to different degrees : indications of a transient
dioxygen complex with
copper - B. -
pubmed
The reactions of the fully reduced , three - electron - reduced , and mixed - valence
cytochrome oxidase with molecular
oxygen have been followed in flow - flash experiments , starting from the
CO complexes , at 445 and 830 nm at pH 7.4 and 25 degrees C. With the fully reduced and the three - electron - reduced
enzyme , four kinetic phases with rate constants in the range from 1 x 10(5 ) to 10(3 ) s-1 can be observed . -
pubmed
It is suggested that
O2 initially binds to reduced
CuB and is then transferred to
cytochrome a3 before electron transfer from cytochrome a /
CuA takes place . -
pubmed
RESULTS : Training significantly increased maximal ventilatory
oxygen consumption ( VO2MAX ) , and there was a decrease in ambulatory
diastolic blood pressure (
DBP ) which did not reach statistical significance . -
pubmed
Atenolol alone significantly reduced ambulatory
systolic blood pressure (
SBP ) and
DBP . -
pubmed
This chemical modification increases the dissociation constant of the
apoflavodoxin -
flavin mononucleotide (
FMN ) complex from 0.4 nM to 2 microM. -
pubmed
The signals at 109.4 ppm and 112.2 ppm due to the cyanylated apoflavodoxin were unstable at 28 degrees C , and they were slowly replaced signals at 114.5 ppm and 115.3 ppm which are attributed to an inactive form of the
apoprotein , which does not bind
FMN . -
pubmed
Differential
dopamine - induced
prolactin mRNA levels in various
prolactin - secreting cell ( sub)populations . -
pubmed
We have examined the effects of
dopamine on
prolactin gene expression using quantitative in - situ hybridization histochemistry in different pituitary cell ( sub)populations separated according to their density on a discontinuous Percoll gradient . -
pubmed
Administration of
dopamine resulted in a drastic reduction in hybridization of
35S - labelled DNA probe complementary to
prolactin mRNA in total pituitary cells and in lactotrophs with low density . -
pubmed
In contrast ,
dopamine significantly stimulated mRNA accumulation in
prolactin - secreting cells with high density compared with other cell layers . -
pubmed
Prolactin - secreting cells with high and low density clearly show functional heterogeneity in their response to
dopamine . -
pubmed
In nonstripped colon , the action of
serotonin (
5 - hydroxytrytamine [ 5 - HT ] ) on short - circuit current was inhibited by a factor of 60 % by
tetrodotoxin ( P less than 0.05 ; t test ) and was also inhibited by the
5HT2 antagonist
ketanserin ( p less than 0.05 vs
serotonin ; t test ) and the
5 - HT3 antagonist
ICS 205 - 930 ( p less than 0.05 vs
serotonin , t test ) . -
pubmed
Surface
TM activity was increased by active
phorbol esters ( 10(-8 ) M , 24 - 48 h ) , analogs of
cAMP ( 1 - 10 mM , 4 h ) , and
forskolin ( 10(-5 ) M , 24 - 48 h ) . -
pubmed
Up - regulation of
TM in HUVECs by
4 beta - phorbol 12 - myristate 13 - acetate (
PMA ) and
dibutyryl cAMP (
dBcAMP ) was due to de novo synthesis of
TM protein resulting from increased
TM mRNA levels . -
pubmed
In addition ,
PMA effects on surface
TM activity are biphasic , with an initial reduction followed by a significant enhancement . -
pubmed
Continuous exposure to
oestradiol-17 beta ( 1.8 nmol / l ) for 9 days in serum - free medium resulted in a decrease in cytosolic
oestrogen receptors with an associated nuclear accumulation of
oestrogen receptors . -
pubmed
Thus , as previously reported for vaginal epithelial cells ,
oestrogen , despite its stimulation of specific product synthesis (
progestin receptors ) , did not increase proliferation of endometrial luminal epithelial cells in this culture system . -
pubmed
Without
SA or CA ,
GM was not absorbed . -
pubmed
However ,
GM absorption was marked when 90 mg of solid SA or CA was added ( the bioavailability of
GM was 58 % with
SA , and 59 % with CA ) . -
pubmed
The enhancing effect of
SA or CA ( 30 mg ) in solid or aqueous solution form on
GM absorption was evaluated using the Type I suppository . -
pubmed
In the case of
SA , the highest plasma
GM level ( Cmax 15.3 + /- 1.7 micrograms / ml , AUC0 - 4 27.3 + /- 3.9 h.micrograms/ml ) was obtained following coadministration of powdered GM and
SA ; the plasma
GM level ( Cmax 1.5 + /- 0.6 micrograms / ml , AUC0 - 4 3.0 + /- 1.3 h.micrograms/ml ) following the administration of a solution of
GM and
SA was significantly decreased as compared with the results using the powdered form . -
pubmed
A marked increase in the enhancing effect of
SA on the rectal
GM absorption was found following use of Type
II suppositories when
GM and
SA were coadministered in solution form . -
pubmed
However , the
GM absorption after coadministration of
GM and CA using Type
II suppositories was not significantly different from the absorption resulting from use of Type I suppositories . -
pubmed
Our results suggest that the form and concentration of drug should not be ignored in evaluating the enhancing effects of
SA or CA on the rectal absorption of poorly absorbed drugs such as
GM . -
pubmed
Arecoline and
arecaidine propargyl ester (
APE ) as well as their corresponding
N - demethyl derivatives ,
guvacoline (
norarecoline ) and
guvacine propargyl ester , acted as full agonists at both
atrial and ileal muscarinic receptors ( range of pD2 - values 6.09 - 8.07 ) . -
pubmed
Treatment of infected L cells with 10 micrograms / ml
cytochalasin B ( CB ) was found to promote a rapid relocalization of
viral glycoproteins on the cell surface . -
pubmed
Acetaminophen as a cosubstrate and inhibitor of
prostaglandin H synthase . -
pubmed
At the concentrations tested ,
NOP ,
NPD ,
OPD ,
MPD and ANL were active in one or more assays . -
pubmed
A panel of 10
FITC - labelled
lectins (
MPA , PNA ,
ConA , DBA , SBA , RCA-120 , WGA ,
UEA ,
GS - I ,
GS - II+ ) was applied to cryosections of seven specimens of
normal urothelium . -
pubmed
MPA , RCA ,
UEA and
GS - I showed the most marked increase in fluorescence intensity from basal to superficial cells as observed microscopically and quantified by flow cytometry . -
pubmed
The purification led to an homogeneous
hydrogenase preparation with a specific activity of 269 U / mg
protein (
methylene blue reduction ) and a yield of 45 % . -
pubmed
During purification and storage the
enzyme was optimally stabilized by the presence of 0.2 mM
MnCl2 . -
pubmed
The acceptors reduced include
viologen dyes ,
flavins ,
quinones ,
cytochrome c ,
methylene blue ,
2,6 - dichlorophenolindophenol ,
phenazine methosulfate and
ferricyanide . -
pubmed
eutrophus H16 it was demonstrated that this type of
hydrogenase in its solubilized , purified state is able to catalyze also the reverse reaction , the
H2 evolution from reduced
methyl viologen . -
pubmed
The effect of
d - and l - norepinephrine on
rat cardiac phosphorylase activation . -
pubmed
Alanine dehydrogenase activity and growth yield ( grams of cells / mole of
methane ) were highest when the organism was cultured with excess
ammonia , whereas growth yield was lower and
glutamine synthetase was maximal when
ammonia was limiting . -
pubmed
These findings support the view that liver
HBcAg and serum
HBeAg are markers of virus replication , and demonstrate that active
liver disease in
HBsAg - positive patients may occur with or without such markers of replication . -
pubmed
We report our observed change in the pattern of progression of
septicemia to
meningitis and death in nonimmunized
SS children who were not receiving prophylactic
penicillin in the
face of a persistently high incidence of
pneumococcal disease . -
pubmed
In the presence of
catalase , the amounts of
chromium released by phagocytosing and resting macrophages were similar ; in the presence of
superoxide dismutase and
cytochrome c , the amount of
chromium released by phagocytosing macrophages was 13 - 35 % less than the amount of
chromium released by macrophages incubated without the antioxidants . -
pubmed
The aim of this study was to assess the behavior of fasting serum
glucose ,
C - peptide levels and OGTT in
pancreatic cancer follow - up . -
pubmed
[ Evaluation of
serum adenosine deaminase as a prognostic marker in the treatment of
human immunodeficiency virus infection with
zidovudine ] . -
pubmed
Therapy with
AZT decreased
ADA activity : 21.6 + /- 5.4 vs. 15.2 + /- 4.3 U / l ( p & lt ; 0.001 ) and correlated with an increase in
CD4 counts : 187 + /- 105 vs. 353 + /- 145 / mm3 ( p & lt ; 0.001 ) and in
CD4 /
CD8 ratio : 0.188 + /- 0.10 vs. 0.382 + /- 0.18 ( p & lt ; 0.001 ) . -
pubmed
Use of a polymorphic
dinucleotide repeat sequence to detect non - blastomeric contamination of the
polymerase chain reaction in biopsy samples for preimplantation diagnosis . -
pubmed
MEASUREMENTS : Serum thyroid hormone levels ,
serum thyrotropin receptor antibody titer , and thyroidal
radioiodine uptake . -
pubmed
L - NAME significantly decreased
sodium excretion ( 1.38 + /- 0.41 to 0.36 + /- 0.14 microEq / min and 1.19 + /- 0.46 to 0.30 + /- 0.05 microEq / min , respectively ) , fractional excretion of
lithium ( 25.7 + /- 1.7 % to 16.7 + /- 2.3 % and 25.6 + /- 4.0 % to 18.2 + /- 1.7 % ) , and renal interstitial hydrostatic pressure ( 6.4 + /- 1.4 to 3.2 + /- 0.9 mm Hg and
6.3 + /- 1.8 to 2.7 + /- 0.9 mm Hg ) in servocontrolled and nonservocontrolled groups . -
pubmed
After injection of UK ,
NEP decreased significantly and urinary
kinins ,
cGMP , urine volume ( UV ) and urinary
sodium excretion ( UNaV ) increased significantly . -
pubmed
With a pretreatment of
L - NMMA , injected UK decreased
NEP and increased
kinins , while urinary
cGMP , UV and UNaV did not increase . -
pubmed
Moreover , changes in urinary
cGMP do not reflect the changes in plasma
ANP , but rather , those in
NO under this condition . -
pubmed
Focal accumulations of mononuclear cells containing
IL-2 producing cells were readily detected as early as 2 - 3 h following local challenge with
hapten in animals primed at skin but not at buccal sites . -
pubmed
Known as
osmotic stress protein 94 , or
Osp94 , this 2935 - base pair cDNA encodes an 838 -
amino acid protein that shows greatest homology to the recently discovered
hsp110 / SSE gene subfamily . -
pubmed
Like the
hsps ,
Osp94 has a putative
amino - terminal
ATP - binding domain and a putative
carboxyl - terminal peptide - binding domain . -
pubmed
The in vitro translated
Osp94 product migrated as a 105 - 110 - kDa protein on
SDS - polyacrylamide gel electrophoresis . -
pubmed
Treatment of
woodchuck hepatitis virus infection in vivo with
2 ' , - 3 '- dideoxycytidine (
ddC ) and
2 ',- 3 '- dideoxycytidine monophosphate coupled to
lactosaminated human serum albumin (
L - HSA ddCMP ) . -
pubmed
The present study examined the effects of lesions of the central nucleus of the amygdala ( CeA ) on the acquisition of a new response with conditioned reinforcement (
CR ) and its potentiation by intra - accumbens infusions of
d - amphetamine ( 1 , 3 , 10 , and 20 microg / microl ) . -
pubmed
Remifentanil is a new synthetic opioid with direct action on
mu - opioid receptors . -
pubmed
The hydrolysis of
remifentanil produces a metabolite with very weak
opioid receptor activity that does not contribute to the effects of
remifentanil . -
pubmed
The authors examined the effect of four different kinds of
contrast media ( ionic / non - ionic , monomer / dimer ) on the activation of the complement ( C ) system ( haemolytic activity and
anaphylatoxin generation ) in vitro . -
pubmed
In addition , the authors compared the effect of contrast media on
inulin - mediated generation of the
anaphylatoxin derivative
C3a des Arg in sera from urticarial reactors and their non - reacting controls . -
pubmed
No significant difference between the urticarial reactors and non - reactors in the inhibition of
C3a des
Arg generation was observed . -
pubmed
These include , amongst others , the
platelet - derived growth factor receptor , the
fibroblast growth factor receptor , and the nonreceptor
c - Src tyrosine kinase . -
pubmed
Leech immunocytes contain
proopiomelanocortin :
nitric oxide mediates hemolymph
proopiomelanocortin processing . -
pubmed
No
dibasic amino acid residues were found at the C terminus of the
gamma - and beta - MSH peptides . -
pubmed
In contrast , the leech
alpha - MSH was flanked at its C - terminal by the
Gly - Arg - Lys amidation signal . -
pubmed
ACTH and
corticotropin - like intermediary pituitary peptide were also C - terminally flanked by
dibasic amino acid residues . -
pubmed
Nonsteroidal anti - inflammatory drugs may delay the repair of gastric mucosa by suppressing
prostaglandin - mediated increase of
hepatocyte growth factor production . -
pubmed
Prostaglandins (
PGs ) ,
hepatocyte growth factor (
HGF ) , and induction of
cyclooxygenase (
PG synthetase ,
COX ) play important roles in the repair process of gastric mucosa . -
pubmed
Among various potential stimulants tested ,
interleukin-1 beta (
IL-1 beta ) dramatically increased
PGE2 production and significantly stimulated
HGF production . -
pubmed
IND significantly reduced both basal and
IL-1 beta - induced
PGE2 release and
HGF production . -
pubmed
This study determined whether
estradiol regulates
insulin - like growth factor ( IGF )- I and
IGF binding proteins (
IGFBPs ) in the pituitary gland , hypophyseal stalk median eminence ( SME ) , and circulation concomitantly with effects on
LH . -
pubmed
Estradiol suppressed serum
LH to a greater extent during anestrus than after onset of the breeding season ( Days 60 and 75 ) . -
pubmed
Amounts of mRNA for
LHbeta subunit were decreased by
estradiol , but mRNA for alpha and
FSHbeta subunits were not affected . -
pubmed
Estradiol increased serum
IGF - I ,
IGFBP-3 , and
IGFBP-4 throughout the treatment period , but it did not influence other
IGFBPs in serum . -
pubmed
In response to
estradiol , pituitary
IGFBP-2 tended to increase and mRNA for
IGFBP-2 increased twofold . -
pubmed
Other
IGFBPs in the pituitary gland were not influenced by
estradiol . -
pubmed
In the SME ,
IGFBP-2 ,
IGFBP-5 , and the 40 - kDa
IGFBP-3 were increased by
estradiol . -
pubmed
Estradiol influences on gonadotroph function may be mediated by alterations in the
IGF system . -
pubmed
These results suggest that the
etidronate treatment for 2 weeks beforehand influenced the effects of subsequent exercise training on maintaining the
BMD in the proximal femur and the trabecular bone area of the tibia . -
pubmed
Maximal positive
GluR2 promoter activity required both
Sp1 and
NRF-1 cis elements and an interelement
nucleotide bridge sequence . -
pubmed
The deleterious effects of the abasic mutations are not relieved by lowering the temperature , by increasing the concentration of monovalent or divalent metal ions , or by adding
polyamines , in contrast to effects observed with
protein enzymes and large RNA
enzymes . -
pubmed
Biochemical analysis of the
Cas - Br - E Env isoforms indicated that they result from differential processing of
N - linked sugars . -
pubmed
Methylene group selectivity data showed only minor differences in the stationary - phase characteristics between the small- and large - pore size
C18 columns . -
pubmed
The true eluting power of micellar mobile phases was revealed with wide - pore stationary phases and was demonstrated by the separation and elution of an extended series of
alkylphenones on
C18 columns . -
pubmed
The expression of
conglutin gamma was studied by partial
amino acid sequencing of the mature seed protein and by
nucleotide sequencing of
reverse transcriptase -
polymerase chain reaction products from various tissues during the plant life cycle . -
pubmed
The inhibitory effect of CART peptide 55 - 102 on
Ca ( 2 + ) signaling is likely mediated by an inhibition of
L - type voltage - gated Ca ( 2 + ) channel activity via a G - protein - dependent pathway . -
pubmed
These results indicate that voltage - gated
Ca ( 2 + ) channels in hippocampal neurons are targets for
CART peptide 55 - 102 and suggest that
CART peptides may be important in physiology and behavior mediated by the hippocampus , such as certain forms of learning and memory . -
pubmed
RESULTS : Tx had postabsorptive and
insulin - stimulated
glucose ,
leucine , and free
fatty acid metabolism , as well as IMCL content , similar to that of CON . -
pubmed
A defective
insulin - dependent suppression of the endogenous
leucine flux ( index of proteolysis ) was also evident during the
clamp in D - Tx . -
pubmed
CONCLUSIONS : Heart transplantation , notwithstanding the immunosuppressive therapy , was characterized by a normal postabsorptive and
insulin - stimulated
glucose ,
leucine , and free
fatty acid metabolism in Tx . -
pubmed
A natural gradient experiment with dissolved
MTBE - containing
gasoline in the shallow , aerobic sand aquifer at Canadian Forces Base (
CFB ) Borden ( Ontario , Canada ) from 1988 to 1996 suggested that biodegradation was the main cause of attenuation for
MTBE within the aquifer . -
pubmed
Quinoline resistance in
malaria is frequently compared with
P - glycoprotein - mediated multidrug resistance ( mdr ) in mammalian cells . -
pubmed
Genetic analysis of putative
quinoline resistance genes did not suggest an association between known point mutations in
pfcrt and
pfmdr1 and
NP30 sensitization activity . -
pubmed
Class IC drugs (
ethacizine ,
disopyramide ) caused worsening of qualitative parameters of
SA ECG and appearance of ventricular late potentials . -
pubmed
Semen samples and serum for reproductive hormones (
testosterone ,
luteinizing hormone and
follicle - stimulating hormone ) were collected at baseline , after 3 months and at the end of treatment . -
pubmed
[ The duration of effects of a
zinc insulin protaminate ] . -
pubmed
We propose a new mechanism of metabolic compensation of seasonal temperature variations - cold acclimation results in changes in the kinetic and thermodynamic properties of
LDH in a way that favours aerobic metabolism through reduction of the competition of
LDH for
pyruvate in normoxic conditions . -
pubmed
Ethanol shows antiketonaemic effects changing the cellular redox potential , inhibiting beta - oxidation of
fatty acids , stimulating the release of
insulin and inhibiting the release of its antagonist . -
pubmed
Pigpen is a cellular binding protein of therapeutic
oligonucleotides . -
pubmed
Although co - localization of
pigpen with
phosphorothioate ( PS ) ON was not observed for the first hour after ON uptake , co - localization was observed 8 h later . -
pubmed
One -
pot synthesis of large
FePt nanoparticles from metal salts and their thermal stability . -
pubmed
One -
pot synthesis of
FePt nanoparticles larger than 5 nm with controlled composition has been developed by the
polyol reduction of
platinum acetylacetonate and
iron acetylacetonate in excess ligands . -
pubmed
When mitochondrial
oxidases were blocked , illumination of the algae reversed the effect of the inhibitors on the
ATP and
glucose 6 - phosphate concentrations . -
pubmed
We conclude that
hyperhomocysteinemia enhances susceptibility to
arterial thrombosis through a mechanism that is not caused by loss of endothelium - derived
nitric oxide but may involve oxidative stress and impairment of the
protein C anticoagulant pathway . -
pubmed
SPK activity assay was used to evaluate the effect of
alphastatin on ECs . -
pubmed
CONCLUSION : Downregulating ECs
SPK activity may be one of the mechanisms that
alphastatin inhibits
gastric cancer angiogenesis . -
pubmed
Dual - specificity phosphatases (
DSPs ) , which dephosphorylate proteins at
Ser /
Thr as well as
Tyr residues , are thought to be involved in critical signaling events such as control of
MAP kinases (
MAPKs ) . -
pubmed
LDP-4 is composed of 211
amino acids with a predicted molecular mass of 23.9 - kDa . -
pubmed
Here we describe the crystal structure of
GlpG in a more open conformation , where the capping loop
L5 has been lifted , exposing the previously buried and catalytically essential
Ser-201 to outside aqueous solution . -
pubmed
The loop movement also destabilizes a hydrophobic side chain (
Phe-245 ) previously buried between transmembrane helices
S2 and
S5 and creates a side portal from the lipid to
protease active site . -
pubmed
BAEP measurements recorded at 85 dB both at entry into and after the study were not significantly different among the groups , although a sufficient response to
iron treatment was achieved in
iron - deficient groups ( Group I and Group
II ) . -
pubmed
A selective
CRF1 receptor antagonist (
NBI27914 ) or vehicle was administered systemically ( i.p . ) -
pubmed
Most of these cells exhibited activation evidenced by an increased reactive
oxygen species generation and high expression of the
Gr1 +/ Mac1 + markers . -
pubmed
An
amine - targeted iTRAQ tag labels tryptic peptides generated from the proteins and
protease cleavage products of secreted proteins , as well as protein domains shed from the cell membrane or pericellular matrix of
protease - transfected cells that have accumulated in conditioned medium ; a second iTRAQ tag is used for control cells . -
pubmed
A conditional logistic regression model was used to identify risk factors for
MRSA bacteraemia compared with
methicillin - sensitive
SAB and no
bacteraemia in unmatched ( 1:1 ) and matched ( 1:4 ) nested case - control analyses , respectively . -
pubmed
Phase 2 study of
gemcitabine and
irinotecan in metastatic
breast cancer with correlatives to determine
topoisomerase I localization as a predictor of response . -
pubmed
BACKGROUND :
Gemcitabine incorporation into DNA enhances cleavage complexes in vitro when combined with
topoisomerase I inhibitors and demonstrates synergy in
cancer cells when given with
irinotecan . -
pubmed
Correlative studies included characterization of cellular levels and nuclear distribution of
topoisomerase I and pharmacokinetic analysis of
gemcitabine and
irinotecan . -
pubmed
TTR was also down - regulated by the commercial
penta - BDE mixture ,
DE-71 . -
pubmed
In contrast , neither
DE-71 , nor
BDE-47 , -99 , or -100 altered the mRNA levels of the
TRs or
nAChR alpha-7 . -
pubmed
Deletion of
Fphog1 , a
HOG - type
MAP kinase gene resulted in further increases in
FUM1 and
FUM8 expression under
nitrogen starvation ( absence of any N - source ) conditions . -
pubmed
Fumonisin B1 (
FB1 ) production paralleled with increased
FUM gene expression : significant amounts of
FB1 were measured in culture filtrates of the
DeltaFphog1 deleted mutant after five days culturing , whereas only traces of
FB1 could be detected in filtrates of the wild type and the restored
strain (
R1 ) complemented with the
wild - type Fphog1 - 24 gene . -
pubmed
The up - regulation of
fumonisin biosynthesis genes in the
DeltaFphog1 mutant could be explained by the increased sensitivity of these
strains to N -
starvation stress that appears in the absence of an intact
HOG - type
MAPK pathway . -
pubmed
In addition ,
HMC05 significantly inhibited production of reactive
oxygen species ( ROS ) and
nuclear factor ( NF )- kappaB activation by
TNF - alpha . -
pubmed
Those inhibitory effects of HMC05 ( 1 - 10 mug mL(-1 ) ) on the
TNF - alpha - induced inflammatory event was similar to those of
berberine ( 1 - 10 muM ) , which is a major component of HMC05 and one of herbal compounds known to have vasorelaxing and lipid - lowering activities . -
pubmed
Role of the
AMPKgamma3 isoform in
hypoxia - stimulated
glucose transport in glycolytic skeletal muscle . -
pubmed
Skeletal muscle
glucose transport is regulated via the canonical
insulin - signaling cascade as well as by energy - sensing signals . -
pubmed
5 '- AMP - activated protein kinase (
AMPK ) has been implicated in the energy status regulation of
glucose transport . -
pubmed
Taken together , we provide direct evidence for a role of the
AMPKgamma3 isoform in
hypoxia - mediated
glucose transport in glycolytic muscle . -
pubmed
Biochemical characteristics in term of quantities of protein and
nitric oxide (
NO ) did not differ significantly , whereas the quantity of
glucose ,
acid phosphatase (
ACP ) and
vitamin E were significantly higher in the T(2 ) group . -
pubmed
CD8(+ ) T cells are activated upon presentation of
antigens from the cytosol . -
pubmed
Physiological observations upon a larval Eustrongylides ; influence of
oxygen tension on the aerobic and
post - anaerobic
oxygen consumption . -
pubmed
The in vitro exposure of kidney proximal tubular cells to physiological concentrations of C - peptide activates
extracellular signal - regulated kinase ,
phosphatidylinositol 3 - kinase ,
protein kinase C , elevates intracellular
calcium , and stimulates
transcription factors NF - kappaB and
peroxisome proliferator - activated receptor - gamma . -
pubmed
Immersion in a simulated body solution (
SBF ) solution was used to evaluate the bioactivity of the plasma - treated
PET /
TiO ( 2 ) samples in vitro . -
pubmed
Analytical and microstructural investigations of
SBF results , showed considerable higher rates of
apatite formation on the plasma - treated
PET /
TiO ( 2 ) compared to the untreated films . -
pubmed
The BH3 - only protein
Bad confers
breast cancer taxane sensitivity through a nonapoptotic mechanism . -
pubmed
Unexpectedly ,
Bad did not induce the mitochondrial apoptotic machinery in response to
taxane treatment . -
pubmed
As dividing cells are the targets of
taxane therapy ,
Bad - stimulated proliferation may be a marker of
taxane sensitivity . -
pubmed
They also suggest that cells expressing
Bad are more sensitive to
taxanes because of their altered cell cycle dynamics and reveal a clinically relevant proliferative role of
Bad in
breast cancer . -
pubmed
After
curcumin treatment ,
BAX and
BAD were up - regulated ,
BCL-2 ,
BCL - X ( L ) and
XIAP were down - regulated . -
pubmed
Apoptotic cell
death induced by
curcumin was significantly reversed by pretreatment with ROS scavenger or
caspase-8 inhibitor . -
pubmed
RESULTS : The T124A mutation alone and the combination T124A / L101I were more frequent in
raltegravir - failing patients than in
INI - naive patients ( 39.3 % versus 24.5 % , respectively , P = 0.005 for T124A and 20.2 % versus 10.0 % , respectively , P = 0.008 for T124A / L101I ) . -
pubmed
The optimal temperature was in the range 45 - 55 C. All three
proteases were active towards
azokeratin and were inhibited by
EDTA , suggesting that they are
keratinolytic metalloproteases . -
pubmed
The proteolytic activity of
K12 was stimulated by organic solvents and the detergent
SDS , suggesting its potential application for detergent formulations and peptide synthesis . -
pubmed
The number of immature flukes was positively correlated with values of
aspartate aminotransferase (
AST ) ,
gamma - glutamyltransferase (
GGT ) ,
LDH ,
GLDH ,
urea and
triglycerides . -
pubmed
The number of migratory lesions was positively correlated with
GGT ,
GLDH ,
globulin and
urea values . -
pubmed
Thermo - sensitive
transient receptor potential vanilloid channel-1 regulates intracellular
calcium and triggers
chromogranin A secretion in pancreatic neuroendocrine BON-1
tumor cells . -
pubmed
Transient receptor potential channels (
TRPs ) regulate
tumor growth via
calcium - dependent mechanisms . -
pubmed
The ( thermosensitive )
capsaicin receptor
TRPV1 is overexpressed in numerous highly aggressive
cancers . -
pubmed
Intracellular free
Ca ( 2 + ) ( [
Ca ( 2 +)]( i ) ) was measured by
fura-2 ;
TRPV1 channel currents by the planar patch -
clamp technique . -
pubmed
Pharmacological blockade of
TRPs by
La ( 3 + ) ( 100 muM ) or by
ruthenium - red (
RuR ) or by
capsazepine (
CPZ ) ( both at 10 muM ) suppressed the
capsaicin (
CAP)- or heat - stimulated increase of [
Ca ( 2 +)]( i ) in
NET cells . -
pubmed
Furthermore ,
CAP ( 10 muM ) stimulated
CgA secretion , which was inhibited by
CPZ or by
RuR ( both 10 muM ) . -
pubmed
La ( 3 + ) potently reduced both stimulated and the basal
CgA secretion . -
pubmed
Activation of
TRPV1 translates into changes of intracellular
Ca ( 2 + ) , a known mechanism triggering the secretion of
CgA. -
pubmed
In both WT and
synapsin DKO beta - cells the
Ca ( 2 + ) current peaked at 0 mV and measured peak current ( I(p ) ) and net charge ( Q ) were of similar magnitude . -
pubmed
MEASUREMENTS : The primary outcomes were
25 -( OH ) D and
PTH levels at 6 and 12 months . -
pubmed
Parathyroid hormone levels at 12 months decreased with an increasing dose of
vitamin D ( 3 ) ( P = 0.012 ) . -
pubmed
Study of surface activity of
piroxicam at the interface of
palm oil esters and various aqueous phases . -
pubmed
The aim of this study was , firstly , to assess the surface and interfacial tension behaviour of newly synthesised
palm oil
esters and various pH
phosphate buffers . -
pubmed
The study revealed that
piroxicam has no effect on surface tension values of all pH
phosphate buffers and
palm oil
esters . -
pubmed
The
arginine methyltransferase
PRMT5 -
MEP50 is required for embryogenesis and is misregulated in many
cancers . -
pubmed
However , the mechanism by which
PRMT5 utilizes
MEP50 to discriminate substrates and to specifically methylate target
arginines is unclear . -
pubmed
HO-1 , a microsomal enzyme , catalyzes the breakdown of pro - oxidant
heme , which is released from
heme proteins to equimolar quantities of
iron ,
carbon monoxide , and
biliverdin . -
pubmed
Biliverdin is converted to
bilirubin by
biliverdin reductase . -
pubmed
The beneficial effects of
HO-1 expression are not merely due to
heme degradation but are also attributed to the cytoprotective properties of the byproducts of the reaction . -
pubmed
Bone - seeking tracers like
99mTc - DPD and
pyrophosphate are beginning to have a role in imaging
transthyretin cardiac amyloidosis . -
pubmed
GROWING POINTS AND FURTHER RESEARCH :
99mTc - DPD has a high sensitivity and specificity to cardiac
transthyretin amyloid deposits and its role in early diagnosis of this condition is under investigation . -
pubmed
2 -( 2 - Phenylmorpholin-4 - yl ) pyrimidin-4 ( 3H )- ones ; a new class of potent , selective and orally active
glycogen synthase kinase-3beta inhibitors . -
pubmed
A series of
2 -( 2 - phenylmorpholin-4 - yl ) pyrimidin-4 ( 3H )- ones was synthesized and examined for their inhibitory activity against
glycogen synthase kinase-3beta (
GSK-3beta ) . -
pubmed
We hypothesized that an
aldosterone excess was associated with abnormalities in urinary proteome , specific for
PA subtypes . -
pubmed
Single
nucleotide polymorphisms ( SNPs ) in the regulatory regions of
TPH2 gene may affect gene expression and biosynthesis of
5 - HT triggering to various
neuropsychiatric disorders related to 5 - HT dysfunction . -
pubmed
Rab9 plays a vital role in regulating the transport of
mannose 6 - phosphate receptors from late endosomes to the trans - Golgi network through interactions with various effectors . -
pubmed
Here , we report the crystal structure of
GTP - bound
Rab9A in complex with the
Rab - binding domain ( RBD ) of the effector
RUTBC2 . -
pubmed
Dietary GI and
GL were independently associated with risk for
endometrial cancer but
carbohydrate intake was unrelated to risk . -
pubmed
This study suggests that intake of high
GL or GI foods , but not
carbohydrates per se , may increase risk for
endometrial cancer . -
pubmed
PMA primed PBTLs for polarization under flow , with
protein kinase C ( PKC )- delta enriched in the leading edge ,
PKC - betaI in the microtubule organizing center , and
PKC - betaII in the uropod and peripheral region . -
pubmed
RESULTS : Serum
osteocalcin level was independently associated with menopausal status , bone mineral density ,
calcium ,
phosphate ,
alkaline phosphatase , fasting blood
glucose ,
triglyceride ,
low - density lipoprotein cholesterol ,
alanine aminotransferase ,
gamma - glutamyltransferase , and
NAFLD . -
pubmed
The
ER stress response is an adaptive mechanism that allows
tumor cells to survive in the harsh growth conditions inherent to the
tumor milieu such as low
oxygen (
hypoxia ) , low pH and low levels of
glucose . -
pubmed
Following collapse of the
GC , memory T cells persisted in the outer follicle where they scanned
CD169(+ ) subcapsular
sinus macrophages . -
pubmed
These data suggest that Tfh cells integrate their
antigen - experience history to focus T cell help within the
GC during primary responses but act rapidly to provide systemic T cell help after re - exposure to the
antigen . -
pubmed
Qiliqiangxin Protects Against
Cardiac Ischemia -
Reperfusion Injury via Activation of the
mTOR Pathway . -
pubmed
Expression levels of
phospho -
mTOR (
Ser2448 ) ,
mTOR ,
phospho-4EBP (
Ser65 ) ,
4EBP ,
phospho -
Akt (
Ser473 ) and
Akt were detected by Western blotting . -
pubmed
Moreover , western blotting analysis demonstrated that QL activated the
mTOR pathway , while
mTOR inhibition via
Rapamycin abolished the protective effects of QL against I / R
injury . -
pubmed
To engineer biocompatible materials from beta - cyclodextrin (
beta - CD ) , in this study , we synthesized acetalated beta - CDs ( Ac - betaCDs ) by one -
pot acetalation using
2 - ethoxypropene as an acetonation reagent , which can be further processed into nanoparticles ( NPs ) via the emulsion technique . -
pubmed
We also demonstrated increased
serotonin fibers and
synaptophysin in direct contact with motor neurons . -
pubmed
We showed that
DHA significantly upregulates miR-21 and downregulates
PTEN in corticospinal neurons . -
pubmed
Downregulation of
PTEN and upregulation of phosphorylated
AKT by
DHA were also seen in primary cortical neuron cultures and were accompanied by increased neurite outgrowth . -
pubmed
We show that
DHA significantly upregulates miR-21 and phosphorylated
AKT , and downregulates
phosphatase and tensin homolog (
PTEN ) , which is involved in suppressing anatomical plasticity , in corticospinal neurons and in primary cortical neuron cultures . -
pubmed
Finally , they support the hypothesis that
ARFs are a crucial component of the
auxin signaling pathway , which regulates a wide range of physiological processes . -
pubmed
Among the resistant haplotypes , allelic progression involving an additional cis - acting mutation and gene duplication evolved a
CYP9M10 haplotype associated with extremely high transcription and strong
pyrethroid resistance . -
pubmed
To achieve the same effect , at least 500 mug / mL
Cur -
NP alone was needed . -
pubmed
Hb Constant Spring (
Hb CS ,
HBA2 : c.427 T & gt ; C ) is a common
nondeletional alpha - thalassemia ( alpha - thal ) that results from a
nucleotide substitution at the termination codon of the alpha2 -
globin gene . -
pubmed
DNA sequencing of the alpha -
globin genes found that both partners were
Hb CS carriers and the fetus was an Hb
CS homozygote . -
pubmed
The following discussion , derived from an extensive literature search using the PubMed database , applying the terms incretin , GLP-1 , exenatide ,
liraglutide ,
albiglutide ,
dulaglutide ,
lixisenatide ,
semaglutide , and
taspoglutide , provides a comprehensive review of existing and upcoming molecules in the
GLP-1 RA class in terms of their structure , pharmacological profiles , efficacy , safety , and convenience . -
pubmed
Among the standard functionals , omegaB97X - D and
SOGGA11X yield accurate charge reorganization energies in comparison with
IP - EOM - CCSD values . -
pubmed
Together , our results reveal that
S1PR1 contributes to survival and growth mechanisms in injured RGCs by regulating the mammalian target of
rapamycin pathway . -
pubmed
Synthesis ,
cytotoxicity and
carbonic anhydrase inhibitory activities of new
pyrazolines . -
pubmed
A series of
polymethoxylated - pyrazoline benzene sulfonamides were synthesized , investigated for their cytotoxic activities on
tumor and non -
tumor cell lines and inhibitory effects on
carbonic anhydrase isoenzymes (
hCA I and
hCA II ) . -
pubmed
Although
tumor selectivity (
TS ) of the compounds were less than the reference compounds
5 - Fluorouracil and
Melphalan ,
trimethoxy derivatives 4 , 5 , and 6 were more selective than
dimethoxy derivatives 2 and 3 as judged by the
cytotoxicity assay with the cells both types originated from the gingival tissue . -
pubmed
The compound 6 (
4 -[ 3 -( 4 - methoxyphenyl )- 5 -( 3,4,5 - trimethoxyphenyl )- 4,5 - dihydro-1H - pyrazol-1 - yl ] benzene sulfonamide ) showed the highest
TS values and can be considered as a lead molecule of the series for further investigations . -
pubmed
All compounds synthesized showed superior
CA inhibitory activity than the reference compound
acetazolamide on
hCA I , and II isoenzymes , with inhibition constants in the range of 26.5 - 55.5 nM against
hCA I and of 18.9 - 28.8 nM against
hCA II , respectively . -
pubmed
KAT6A - deficient CD8(+ ) T cells downregulated surface
CD8 co - receptor expression during clonal expansion , a finding linked to reduced Cd8alpha transcripts and histone -
H3 lysine 9 acetylation of the Cd8 locus . -
pubmed
Moreover , the axons of identified
PV+/
Scgn+ interneurons preferentially targeted the
somata of
SPNs of the so - called ' direct pathway ' , whereas
PV+/
Scgn- interneurons preferentially targeted ' indirect pathway '
SPNs . -
pubmed
Intestinal
bile acid receptors are key regulators of
glucose homeostasis . -
pubmed
Bile acids bind and modulate the activity of transmembrane and nuclear receptors (
NR ) . -
pubmed
Among these receptors , the
G - protein - coupled bile acid receptor 1 (
TGR5 ) and the
NR farnesoid X receptor (
FXR ) are implicated in the regulation of
bile acid , lipid ,
glucose and energy homeostasis . -
pubmed
More precisely , recent studies have shown that
FXR is important for
glucose homeostasis in particular in
metabolic disorders such as
T2D and
obesity . -
pubmed
This review highlights the growing importance of the
bile acid receptors
TGR5 and
FXR in the intestine as key regulators of
glucose metabolism and their potential as therapeutic targets . -
pubmed
The concordances of the GenoType MTBDRplus assay and the conventional DST for the detection of
RIF and
INH susceptibility were 80 % ( 8/10 ) and 86.2 % ( 25/29 ) , respectively . -
pubmed
For
INH - resistant isolates , the highest mutation rate ( 88.8 % ) related to a
katG mutation ( Codon S315T1 ) . -
pubmed
CONCLUSION : The findings of this study revealed that the GenoType MTBDRplus assay has high sensitivity and specificity for the detection of
RIF and
INH resistance . -
pubmed
The crystal structures of title
hydrogen - bonded co - crystals ,
2C12H16O3 C12H10N2 , ( I ) , and
2C13H18O3 C12H10N2 , (
II ) , have been determined at 93 K. In ( I ) , the asymmetric unit consists of one
4 -( n - pent - yloxy ) benzoic acid mol - ecule and one half - mol - ecule of
( E )- 1,2 - bis -( pyridin-4 - yl ) ethene , which lies about an inversion centre . -
pubmed
The asymmetric unit of (
II ) comprises two crystallographically independent
4 -( n - hex - yloxy ) benzoic acid mol - ecules and one
1,2 - bis -( pyridin-4 - yl ) ethene mol - ecule . -
pubmed
Annexin V , a protein with high affinity to
phosphatidylserine ( PS ) in a
calcium dependent manner , has been widely used to probe apoptosis . -
pubmed
Analysis of Novel Interactions between Components of the
Selenocysteine Biosynthesis Pathway ,
SEPHS1 ,
SEPHS2 ,
SEPSECS , and
SECp43 . -
pubmed
The
SECp43 structural model presented here constitutes the basis of future exploration of the protein - protein interactions among early components of the
selenocysteine biosynthesis and incorporation pathway . -
pubmed
Changes in total ,
HDL- , and
LDL -
cholesterol , and urine
8 - isoprostane levels did not differ between the two groups . -
pubmed
METHODS :
Pulmonary function testing results were compared during
apnea induced by
hyperventilation (
CO2 washout ) vs. intravenous
propofol ( 1 dose 10 mg / kg ) . -
pubmed
The
16S rRNA amplicon sequencing analysis revealed differences in beta diversity with a significant reduction in the relative abundances of
short - chain fatty acid (
SCFA ) producers such as Oscillospira and Ruminococcaceae due to
BPA exposure . -
pubmed
The principal steps of intracellular
cytokine staining are as follows : First , cells are activated for a few hours using either a specific peptide or a non - specific activation
cocktail . -
pubmed
Brefeldin A ) is added to retain the
cytokines within the cell . -
pubmed
Ryanodine receptors (
RyRs ) , the large homotetrameric protein complexes , regulate the release of
calcium from intracellular stores into the cytosol and play vital roles in the excitation - contraction coupling of cells . -
pubmed
The changes in RGC spontaneous firing and membrane potential could be reversed by the
tyrosine kinase inhibitor
PP2 , suggesting that
EphB /
ephrinB reverse signaling activation induced RGC hyperexcitability . -
pubmed
Intravitreal pre - injection of either
LY367385 or
MPEP , selective
mGluR1 and
mGluR5 antagonists , also blocked the changes in RGC spontaneous firing and membrane potential . -
pubmed
Furthermore , intravitreal pre - injection of the mixture of
L - NAME ( an
NO synthase inhibitor ) and
XPro1595 ( a selective inhibitor of soluble
TNF - alpha ) could reduce the
EphB2 - Fc injection induced increase in RGC firing , suggesting that Muller cells might be involved in
EphB /
ephrinB reverse signaling activation induced change in RGC hyperexcitability . -
pubmed
In addition ,
LY367385 /
MPEP reduced the numbers of TUNEL - positive RGCs both in
EphB2 - Fc injected and COH retinas . -
pubmed
Neratinib is a
tyrosine kinase inhibitor that has been approved by the US Food and Drug Administration for the treatment of
breast cancer . -
pubmed
This study was carried out to investigate the in vitro and in vivo metabolism of
neratinib using an UHPLC - DAD - Q Exactive Orbitrap -
MS instrument with dd - MS2 on - line data acquisition mode . -
pubmed
Our findings suggested that
neratinib was mainly metabolized via O - dealkylation ( M3 ) , oxygenation (
M8 ) , N - demethylation ( M10 ) , N - oxygenation ( M12 ) ,
GSH conjugation (
M1 , M2 , M4 , M5 , M6 and M7 ) and
N - acetylcysteine conjugation (
M9 and M11 ) . -
pubmed
Vascular hyperreactivity was accompanied by reduced
nitric oxide (
NO ) , and was reversed by incubation with
Dimethylsulfoxide ,
Catalase and
Tempol . -
pubmed
The
NADPH oxidase subunit
gp91phox was increased due to
iron - overload , and incubation with
angiotensin II type-1 receptor (
AT1 ) antagonist
losartan not only reduced oxidative stress but also restored vascular function . -
pubmed
Thus , we concluded that
AT1 pathway plays a role in
pulmonary vascular dysfunction by increasing oxidative stress and reducing
NO bioavailability , thereby contributing to vascular remodeling and
pulmonary hypertension of
iron - overload . -
pubmed
This finding should instigate future studies on the beneficial impacts of in vivo blockade of
AT1 receptor under
iron overload . -
pubmed
savastanoi
QS regulon consisted of only a few loci whereas in Etoletana many putative metabolic genes were regulated by
QS , among which are several involved in
carbohydrate metabolism . -
pubmed
Response was evaluated using the 68Ga -
PSMA - ligand - PET /
CT or
99mTc - iPSMA - SPECT /
CT diagnostic images and
serum PSA levels before and after
177Lu - iPSMA treatment . -
pubmed
The
regulator of chromosome condensation 1 (
RCC1 ) is the
nucleotide exchange factor for a
GTPase called the
Ras - related
nuclear protein , and it is important for nucleo - plasmic transport , mitosis , nuclear membrane assembly , and control of chromatin agglutination during the S phase of mitosis in animals . -
pubmed
Water depth , transparency (
SD ) , electric conductivity , and total
nitrogen were the highest in
spring , while temperature and total
phosphorus were the highest in summer . -
pubmed
During the
spring period , Protozoa were positively correlated with conductivity , transparency (
SD ) , and total
nitrogen . -
pubmed
A 24 - week study demonstrated that
sodium benzoate , a
D - amino acid oxidase (
DAAO ) inhibitor , surpassed placebo in improving cognitive function in early - phase
Alzheimer ' s disease ; however ,
benzoate did not excel placebo in another 6 - week study on
BPSD . -
pubmed
DAAO level ,
amino acids (
L - serine ,
D - serine ,
L - alanine , and
D - alanine ,
glycine ) , and two antioxidants (
catalase ,
superoxide dismutase ) were assayed in peripheral blood . -
pubmed
Results : After
benzoate treatment ,
DAAO inhibition was correlated with
ADAS - cog decrease ( p = 0.034 ) , while baseline
DAAO level was correlated with baseline BEHAVE -
AD score . -
pubmed
HIF-1alpha - dependent miR-424 induction confers
cisplatin resistance on
bladder cancer cells through down - regulation of pro - apoptotic
UNC5B and
SIRT4 . -
pubmed
RESULTS : In this study , we have found for the first time that
cisplatin (
CDDP ) induces the expression of miR-424 in a
HIF-1alpha - dependent manner under normoxia , and miR-424 plays a vital role in the regulation of
CDDP resistance of
bladder cancer cells in vitro . -
pubmed
Moreover , knockdown of
UNC5B or
SIRT4 prohibited
CDDP - mediated proteolytic cleavage of
PARP and also decreased
CDDP sensitivity of these cells . -
pubmed
CONCLUSIONS : Collectively , our current results strongly suggest that miR-424 tightly participates in the acquisition / maintenance of
CDDP - resistant phenotype of
bladder cancer cells through down - regulation of its targets
UNC5B and
SIRT4 , and thus combination chemotherapy of
CDDP plus
HIF-1alpha / miR-424 inhibition might have a significant impact on hypoxic as well as normoxic
bladder cancer cells . -
pubmed
The ileum was processed for the evaluation of
myeloperoxidase (
MPO ) ,
malondialdehyde (
MDA ) , and the protein expression of
occludin and
activated caspase-3 . -
pubmed
RESULTS :
Indomethacin elicited a significant
intestinal damage , associated with a decrease in blood
hemoglobin and an increase in tissue
MPO ,
MDA and fecal
calprotectin . -
pubmed
In this setting , either the
PAR1 agonist or
PAR2 antagonist counteracted these changes , with the exception of
MDA , which was unaffected . -
pubmed
Indomethacin also decreased
occludin and increased activated
caspase-3 expression in ileal tissues . -
pubmed
CONCLUSIONS :
PAR2 is involved in the pathogenesis of
indomethacin enteropathy , through pro - inflammatory mechanisms and an impairment of the intestinal epithelial barrier . -
pubmed
Within the
AMP group , increases in
PA and decreases in NA both uniquely predicted subsequent increases in connectedness throughout treatment . -
pubmed
Correlation of the preoperative MV -
BMI ratio with preoperative Framingham risk score ( FRS ) and
triglyceride /
HDL -
cholesterol ratio was investigated . -
pubmed
RESULTS : Preoperative MV -
BMI showed an inverse correlation with preoperative FRS and
triglyceride /
high - density lipoprotein -
cholesterol ratio . -
pubmed
BACKGROUND :
Programmed cell death protein 1 /
programmed death - ligand 1 (
PD-1 /
PD - L1 ) blocking antibodies including
cemiplimab have generated profound clinical activity across diverse
cancer types . -
pubmed
89Zr - REGN3504 immuno - PET accurately detected a significant reduction in splenic
PD - L1 positive cells following systemic treatment with
clodronate liposomes . -
pubmed
CONCLUSION : This work supports the clinical translation of
89Zr - REGN3504 immuno - PET for the assessment of
PD - L1 expression . -
pubmed
Future clinical studies will aim to investigate the utility of
89Zr - REGN3504 immuno - PET for predicting and monitoring response to anti -
PD-1 therapy . -
pubmed
The rate of autoxidation of native
oxymyoglobin to
metmyoglobin has been examined over the pH range of 4.8 - -12.6 in 0.1 M buffer at 25 degrees C , and some 40 values of the observed first - order rate constant , kobs , are plotted against pH of the solution . -
pubmed
The complicated pH - profile can be best explained by the ' acid - base catalyzed three states model ' , which reveals not only the catalytic role of
hydrogen ions and
hydroxyl ions , but also the involvement of two dissociation groups of
myoglobin molecule in the autoxidation reaction . -
pubmed
Antiproliferative effects of antisense
oligonucleotides directed to the RNA of
c - myc oncogene . -
pubmed
Several groups have reported the use of antisense
oligonucleotides to inhibit
c - myc gene expression and study its biological role . -
pubmed
To lower their concentration and stabilize the antisense effect against
c - myc ,
oligonucleotides were covalently linked to
poly ( L - lysine ) and administered in ternary complexes formed with heparin ( 100 micrograms / ml ) . -
pubmed
A theoretical (
MM2 ) and experimental ( 1H and
13C NMR ) study of
butaclamol hydrochloride in
CDCl3 has been done in order to determine preferred conformations and internal molecular flexibility of this molecule . -
pubmed
13C spin - lattice relaxation time measurements show that most of the molecule is rigid , although there is some degree of mobility in the seven - membered B ring , associated with rapid flipping of the bridging C8 and
C9 carbons between two skewed conformations , which have previously been referred to as conformer A and conformer B ( Laus et al . -
pubmed
Simultaneous injection of
polymyxin B ( 10(-8 ) mol ( kg body weight)-1 ) , an inhibitor of
protein kinase C , with
TPA and
CCK8 reversed the inhibitory effect of the
phorbol ester on CCK8 - induced pancreatic juice flow ,
total protein output and
amylase release . -
pubmed
Prothrombinase assembly was studied on macroscopic planar bilayers consisting of 20 %
dioleoyl - phosphatidylserine (
DOPS ) and 80 %
dioleoyl - phosphatidylcholine (
DOPC ) . -
pubmed
We performed a random assignment , double - blind , placebo - controlled study of
nortriptyline (
NT ) in postpubertal 12- to 17 - year - olds with Research Diagnostic Criteria ( RDC ) and DSM - III major
depressive disorder . -
pubmed
The only observed biochemical change was a significant decrease in
gamma - glutamyl cysteine synthetase the
glutathione synthetic enzyme , in the carcinogen - induced preneoplastic and neoplastic line compared to control . -
pubmed
Reduced
glutathione (
GSH ) and non - protein
sulfhydryl (
NPS ) levels were not reduced in the liver , kidney , or circulating red blood cells ( rbc ) by
helenalin treatment . -
pubmed
Combination of any of these agents , after three days , with
helenalin afforded increases in P-388 cell
GSH and
NPS levels . -
pubmed
Exons encoding the
beta 1 ,
beta 2 and transmembrane domains of a
B - L beta chain have been identified with 63 , 66 and 62 % similarity with the HLA - DQ beta sequence . -
pubmed
Ornithine decarboxylase and
diamine oxidase activities showed maximal increases at 24 h in the striatum of the lesioned side , whereas in the substantia nigra
ornithine decarboxylase attained a very high value as early as 4 h after the operation and
diamine oxidase activity peaked at 48 h. The
enzyme activities returned toward the basal values at 168 h. On the intact side ,
ornithine decarboxylase showed a small increase starting at 4 h and
diamine oxidase was enhanced at 48 h. These results indicate that the stimulation of biosynthetic and degradative enzymes of
polyamine metabolism accompanied by marked and prolonged increases in
putrescine may be essential events in the early phases of neuronal response to
mechanical injury in the CNS . -
pubmed
The two enzymes were separated by
DEAE - cellulose chromatography and called
kinases I and II . -
pubmed
Adenosien 3 ' : 5 '- cyclic monophosphate stimulated the activity of both
enzymes . -
pubmed
In the presence of
adenosine 3 ' : 5 '- cyclic monophosphate ,
protein kinase I dissociated into two subunits : a subunit binding
adenosine 3 ' : 5 '- cyclic monophosphate , and a catalytic subunit . -
pubmed
The mechanism of action of the
cyclic nucleotide on
kinase II appeared to be different from that of
kinase I. -
pubmed
In complete Krebs - Ringer
bicarbonate buffer ( pH 7.4 ) the
calcium - channel - blocking drugs verapamil ( 50 microM ) and
flunarizine ( 50 microM ) had no effect on active
renin release but increased inactive
renin secretion . -
pubmed
In
Ca - free or
Ca - depleted ( + EGTA ) buffers secretion of both active and inactive
renin was increased . -
pubmed
Neither
verapamil nor
flunarizine under these conditions had any additional effect on active
renin secretion but inactive
renin release was stimulated by
verapamil and inhibited by
flunarizine . -
pubmed
Although having no effect in the presence of
Ca ++ ions , the ionophore
A23187 ( 17 microM ) selectively abolished the secretion of inactive
renin in the absence of
calcium . -
pubmed
But there was a great difference in the apparent association rate constant ( kappa a ) between Domain
II and Domain III at pH 8.0 ( the optimum pH of the enzymatic activity ) , and Domain III associated with
TLE -
Se about 10(5 ) times faster than Domain
II . -
pubmed
The pH dependency of kappa a for Domain III and TLE -
Se was maximum around pH 8 , but in the case of Domain
II and TLE -
Se the maximum was around pH 4 . -
pubmed
This suggested that some acidic
amino acid residues had some influence upon the association of Domain
II with
TLE -
Se . -
pubmed
When we examined the interaction between
TLE -
Se and amidated Domain
II in which most of the
aspartic and glutamic acids were amidated for conversion into
asparagine and
glutamine with
NH4Cl and
carbodiimide , the pH dependence of kappa a greatly differed from that obtained for
TLE - Se and native Domain
II , and an almost constant kappa a value ( 10(3)-10(5 ) times higher than that of native Domain
II ) was exhibited between pH 5 and 8 . -
pubmed
At the same time , the dissociation constant at pH 8.0 became about 10(-2 ) times smaller , and thus the affinity between TLE -
Se and amidated Domain
II became stronger . -
pubmed
We tested this hypothesis by analyzing pausing and non - pausing variants of both the pR ' promoter segment and a more active mutant version of
pR ' ; we measured reporter gene expression and used
KMnO4 footprinting to measure directly occupancy of the promoter and pause sites in growing cells . -
pubmed
Activation of
Ca2 + influx by transforming
Ha - ras . -
pubmed
The expression of transforming
p21Ha - ras caused a significant increase in
Mn2 + influx which was blocked by
Cd2 + ,
La3 + ,
niguldipine and the
Ca ( 2 +)- channel blocker SK&
F96365 . -
pubmed
In addition to the enhanced
Mn2 + influx , transforming
p21Ha - ras elicited an increased efflux of the K(+)-congener
86Rb + which was inhibitable by
Ca ( 2 +)- channel blockers and
charybdotoxin , a selective inhibitor of high and intermediate conductance
Ca ( 2 +)- dependent K + channels . -
pubmed
Charybdotoxin did not reduce the increase in
Mn2 + influx by
ras , demonstrating that the activation of
Ca ( 2 +)- dependent K + channels was not required for the sustained
Mn2 +/Ca2 + influx in the presence of transforming
Ha - ras . -
pubmed
In
ras - expressing cells , the
bradykinin - induced
Mn2 + influx and
charybdotoxin sensitive
86Rb + efflux were markedly potentiated . -
pubmed
The increase in the
inositol - 1,4,5 - trisphosphate and
inositol-1,3,4,5 - tetrakisphosphate levels by
ras is not sufficient to explain the elevated
Mn2 + influx . -
pubmed
The mitogenic response to an expression of transforming
Ha - ras was inhibited by the
Ca ( 2 +)- channel blockers not , however , by
charybdotoxin . -
pubmed
These data suggest the existence of an agonist - independent activation of a receptor- or second messenger - operated
Ca2 + channel by transforming
Ha - ras which is necessary for the mitogenic response to the activation of the oncogene . -
pubmed
[ Preservation of
PCNA in different fixatives and demonstration of
PCNA as well as membrane
antigen by double labelling technique ] . -
pubmed
Four
fixatives were used with two different fixation times in order to determine the influence of different fixatives on
PCNA preservation . -
pubmed
The result indicated that the
aldehyde - base fixatives preserved
PCNA excellently and the appropriate
fixative time was 24 hours . -
pubmed
The result of
alcohol - base
fixative on
PCNA preservation seemed unsatisfactory and 10 % buffer
formalin was the best one among the
aldehyde - base fixatives for this preservation . -
pubmed
Additionally , double - labelling technique for demonstrating
PCNA as well as the membrane
antigen in the same slide was established . -
pubmed
Serum osteocalcin ,
serum total alkaline phosphatase , fasting urinary excretion of
hydroxyproline and
calcium , serum
25 - hydroxyvitamin D , and
1,25 dihydroxyvitamin D were assessed . -
pubmed
Since the incidence of
ER does not significantly differ between females and males , the observed sex differences regarding
thyroid tumor incidence may reflect the higher
estrogen serum content in females . -
pubmed
RBI-257 : a highly potent
dopamine D4 receptor - selective ligand . -
pubmed
Dopamine D4 receptor affinity of
iodo - isomers of
RBI-257 ranked : para & gt ; meta & gt ; ortho . -
pubmed
RBI-257 may be a useful probe or radioligand for brain
dopamine D4 receptors . -
pubmed
EPR detection of endogenous
nitric oxide in postischemic heart using lipid and aqueous - soluble
dithiocarbamate -
iron complexes . -
pubmed
Diethyldithiocarbamate (
DETC ) and
Fe formed in ischemic - reperfused myocardium the lipophilic
DETC2 - Fe - NO complex exhibiting an
EPR signal ( g perpendicular = 2.04 and g parallel = 2.02 at 77 K ) with a triplet hyperfine structure at g perpendicular . -
pubmed
Dithiocarbamate - Fe - NO complexes detected by both trapping agents were abolished by the .
NO synthase inhibitor ,
NG - nitro - L - arginine methyl ester . -
pubmed
For the lipoproteins ,
VLDL had the highest level of
triglycerides followed by
IDL , whereas the
LDL particles had the highest levels of
cholesterol esters compared to all other fractions . -
pubmed
After separating the
lipoprotein particles by size ( FPLC ) it was determined that both
LDL and
HDL carried approximately equal levels of
cholesterol . -
pubmed
The recombinant
KIR2 has been crystallized in 9 - 10 %
poly ( ethylene glycol ) methyl ether ( average Mr = 8000 ) , 50mM
HEPES , 8 %
ethylene glycol , 0.5 %
octyl - beta - glucoside , pH 7.5 , at 294 K using the sitting - drop vapour - diffusion method . -
pubmed
Disease activity was assessed by Ritchie articular index , erythrocyte sedimentation rate ( ESR ) and
C - reactive protein (
CRP ) ; laboratory tests were done for
deoxypyridinoline and
bone alkaline phosphatase . -
pubmed
The mean value for
deoxypyridinoline and
bone alkaline phosphatase on entry were 8.4 and 12.5 ; mean values of Ritchie articular index and ESR were initially 10.1 and 59 mm , respectively . -
pubmed
At 3 months
deoxypyridinoline levels had fallen to 6.2 , while
bone alkaline phosphatase had risen to 18.8 . -
pubmed
At 9 months there was significantly reduced
deoxypyridinoline , to 5.5 ( p & lt ; 0.001 ) , while
bone alkaline phosphatase had risen significantly to 30 ( p & lt ; 0.001 ) compared with pre -
MTX assessment . -
pubmed
Whereas cells cultured in
GM - CSF alone were functionally immature , cells incubated with
CD40L or LPS were mature BmDC , as evident by morphology , capacity to internalize
Ag , migration into regional lymph nodes ,
IL-12 secretion , and alloantigen or peptide
Ag presentation in vitro . -
pubmed
The present data support the clinical use of mature , rather than immature ,
tumor Ag - pulsed dendritic cells as
cancer vaccines and identifies
CD40L as a potent stimulus to enhance their in vivo
Ag - presenting capacity . -
pubmed
The
Erm methyltransferases confer resistance to
macrolide ,
lincosamide and
streptogramin B ( MLS ) antibiotics by methylation of a single
adenosine base within bacterial 23 S ribosomal RNA . -
pubmed
The core of the motif has the primary sequence 2056 - GGAHA-2060 , where H is any
nucleotide except
guanosine , and
ErmE methylates at the
adenosine in bold . -
pubmed
Zener 's model of ferromagnetism , originally proposed for
transition metals in 1950 , can explain T(C ) of
Ga ( 1 -)( x ) Mn ( x ) As and that of its
II - VI counterpart
Zn ( 1 -)( x ) Mn ( x ) Te and is used to predict materials with T(C ) exceeding room temperature , an important step toward semiconductor electronics that use both charge and spin . -
pubmed
The method is based on the extractive two - phase
isobutoxycarbonyl (
isoBOC ) derivatization with subsequent solid - phase extraction ( SPE ) for the direct analysis by gas chromatography (
GC ) and gas chromatography - mass spectrometry (
GC -
MS ) . -
pubmed
The resulting
isoBOC derivatives were then recovered by SPE using Chromosorb
P in normal - phase partition mode , followed by direct GC and GC - MS analysis . -
pubmed
protein - directed selectivity in reactions of
hemoglobin with
aryl trimesates . -
pubmed
There are marked differences in the reaction patterns of these compounds with
amino groups in
hemoglobin compared to reactions with
n - propylamine . -
pubmed
The compounds that effectively modify the
protein contain a
carboxyl group ortho to the
phenolic oxygen of the
ester , while materials with meta or para
carboxyl groups give little or no reaction . -
pubmed
On the basis of these observations and the known reaction patterns of
salicylates , it is clear that the environment in the
protein controls the reaction , regardless of the inherent reactivity of the reagent . -
pubmed
We propose that the
carboxyl group positions the reagent critically within the
protein . -
pubmed
We postulate that the early drop - outs in the
olanzapine - treated
parkinsonian group may be attributable to a non - specific effect of the drug as a result of starting at too high a dose , and that the worsening of
parkinsonism following prolonged treatment may have been caused by the drug 's blocking effect on
striatal D2 receptors . -
pubmed
These studies find that the elevation of
corticosterone does not affect the expression of
membrane receptors on macrophages and does not affect the activation of macrophages to produce
cytokines . -
pubmed
In contrast , elevated
corticosterone levels correlate with enhanced T cell proliferation to both mitogens and
superantigens . -
pubmed
There were no significant differences in
cyclic GMP and
NOS analysis . -
pubmed
Nitric oxide blocks fast , slow , and persistent
Na + channels in C - type DRG neurons by S - nitrosylation . -
pubmed
The
NO scavenger
hemoglobin abolished the effects of
papa - NONOate on
Na + currents , indicating that
NO or
NO - related species inhibit these
Na + currents . -
pubmed
Incubation of neurons with
8 - bromo cGMP , a membrane - permeable analogue of
cGMP , and cG - PKI , an inhibitor of
cGMP - dependent protein kinase , had no effect on
papa - NONOate - mediated
Na + current block , demonstrating that
Na + current inhibition is independent of
cGMP . -
pubmed
Alkylation of free
thiols with
N - ethylmaleimide prevented the actions of
papa - NONOate , suggesting that
NO , or a related reactive
nitrogen species , modifies
sulfhydryl groups on
Na + channels or a closely associated protein . -
pubmed
These results demonstrate that inhibition of
Na + currents is not due to the modulation of fast and slow
sodium channel inactivation . -
pubmed
Taken together , these results show that
NO or
NO - related products modify the
sulfhydryl groups on
Na + channels and inhibit
Na + currents by blocking the channel conductance . -
pubmed
Functional role of alternatively spliced
deoxycytidine kinase in sensitivity to
cytarabine of acute myeloid leukemic cells . -
pubmed
Inactivation of
deoxycytidine kinase (
dCK ) plays an important role in
AraC resistance in vitro . -
pubmed
Because these
dCK - spliced variants were only detectable in resistant
AML , it was hypothesized that they might play a role in
AraC resistance in vivo . -
pubmed
However , a cell expressing alternatively spliced
dCK forms that has lost wt
dCK expression is resistant to the cytotoxic effects of
AraC. -
pubmed
CJ-13,981 and
CJ-13,982 , new
squalene synthase inhibitors . -
pubmed
Two new
squalene synthase (
SSase ) inhibitors ,
CJ-13,981 ( I ) and
CJ-13,982 (
II ) , were isolated from the fermentation broth of an unidentified fungus CL 15036 . -
pubmed
Based on FAB - MS and NMR analyses , the structures of I and
II were determined to be
3 - hydroxy-3,4 - dicarboxy-15 - hexadecenoic acid and
3 - hydroxy-3,4 - dicarboxyhexadecanoic acid , respectively . -
pubmed
To test the usefulness of
RuBD as a probe for diffusive processes in calf thymus DNA , we compared the efficiencies of fluorescence resonance energy transfer ( FRET ) using three donors which display lifetimes near 5 ns for
acridine orange ( AO ) , 22 ns for
ethidium bromide ( EB ) and 92 ns for
RuBD , with
nile blue (
NB ) as the acceptor . -
pubmed
The Forster distances for
AO - NB , EB -
NB and
RuBD -
NB donor - acceptor pairs were 42.3 , 52.3 , and 30.6 A , respectively . -
pubmed
Decreased activity and enhanced nuclear export of
CCAAT - enhancer - binding protein beta during inhibition of adipogenesis by
ceramide . -
pubmed
The addition of exogenous
N - acetylsphingosine (
C2 - ceramide ) or increasing endogenous
ceramide levels inhibited the expression of
C / EBPalpha and
PPARgamma , and blocked adipocyte development . -
pubmed
C2 - ceramide did not decrease the cellular expression of
C / EBPbeta , which is required for expression of
C / EBPalpha and
PPARgamma , but significantly blocked its transcriptional activity from a promoter construct after 24 h. The
ceramide - induced decrease in the transcriptional activity of
C / EBPbeta correlated with a strong decrease in its phosphorylation , DNA - binding ability and nuclear localization at 24 h. However ,
ceramide did not change the nuclear level of
C / EBPbeta after a period of 4 or 16 h , suggesting that it was not affecting nuclear import . -
pubmed
Leptomycin B is an inhibitor of
CRM1 /
exportin-1 , and reversed the
ceramide - induced decrease in nuclear
C / EBPbeta at 24 h. Taken together , these data support the hypothesis that
ceramide may inhibit adipogenesis , at least in part , by enhancing dephosphorylation and premature nuclear export of
C / EBPbeta at a time when its maximal transcriptional activity is required to drive adipogenesis . -
pubmed
p42 / p44 MAPKs are intracellular targets of the
CDK inhibitor
purvalanol . -
pubmed
Intensive screening of a combinatorial chemistry library of
2,6,9 - trisubstituted purines has led to the identification of
purvalanol , one of the most potent and selective
CDK inhibitors to date . -
pubmed
p42 /
p44 MAPK activity was visualized using an Elk -
Gal4 luciferase reporter system and
CDK1 activity was detected by the
phosphonucleolin level . -
pubmed
When cells were treated with
purvalanol ,
p42 / p44 MAPK and
CDK1 activities were inhibited in a dose - dependent manner . -
pubmed
Furthermore ,
purvalanol inhibited the nuclear accumulation of
p42 / p44 MAPK , an event dependent on the catalytic activity of these
kinases . -
pubmed
We conclude that the anti - proliferative properties of
purvalanol are mediated by inhibition of both
p42 / p44 MAPK and
CDKs . -
pubmed
Pretreatment of 4LHBMEC with glycosaminoglycan ( GAG)-degrading enzymes or
sodium chlorate demonstrated that
SDF-1 bound to both
HSPGs and
CS / DSPGs in a sulfation - dependent manner , as determined with an
SDF-1 antibody recognizing the
CXCR4 - binding site . -
pubmed
[ Blood
prothrombin during
radiophosphorus and x - ray therapy of
polycythemia and leukoses ] . -
pubmed
Bilirubin levels in
G6PD - deficient neonates were somewhat higher compared to
G6PD - normal babies ( 18.8 + /- 2.4 mg / dl [ 321.5 + /- 41 micromol / l ] vs. 15.7 + /- 3.2 mg / dl [ 268.5 + /- 54.7 micromol / l ] ; P & lt ; 0.05 ) . -
pubmed
Sodium dodecyl sulphate -
polyacrylamide gel electrophoresis (
SDS - PAGE ) and immunoblotting demonstrated that the purified protein was recognized by both
IL-1ra and
IL-10 antibodies . -
pubmed
The merozoite surface protein
MSP-3beta is a member of a family of related merozoite surface proteins , all of which contain a central
alanine - rich domain that is predicted to form a coiled -
coil tertiary structure . -
pubmed
In this first assessment of
PvMSP-3 beta variation between populations , we discovered widespread and significant diversity , mostly within the
alanine - rich central region . -
pubmed
We observed frequent differences in
PvMSP-3 beta gene size , caused by the insertion and/or deletion of several large sequence blocks , as well as numerous single
nucleotide polymorphisms and smaller scale insertions and deletions . -
pubmed
Lamellipodia production , or function , is defective and there is a selective reduction in the level and
tyrosine phosphorylation of
FAK ,
p130Cas ,
Crk , and
Dock180 at nascent focal complexes . -
pubmed
The high dairy diet resulted in decreases in total body fat ( 2.16 kg , p & lt ; 0.01 ) , trunk fat ( 1.03 kg , p & lt ; 0.01 ) ,
insulin ( 18.7 pM , p & lt ; 0.04 ) , and blood pressure ( 6.8 mm Hg systolic , p & lt ; 0.01 ; 4.25 mm
Hg diastolic , p & lt ; 0.01 ) and an increase in lean mass ( 1.08 kg , p & lt ; 0.04 ) , whereas there were no significant changes in the low dairy group . -
pubmed
Thyroid function tests revealed elevated serum
thyroxine and
triiodothyronine as well as low
thyroid stimulating hormone concentrations , confirming the diagnosis of
thyrotoxicosis . -
pubmed
Because
nitric oxide (
NO ) has been reported to play roles in the pathogenesis of
acute lung injury , the present study explores the effects on VILI of
NO derived from chronically overexpressed
endothelial nitric oxide synthase (
eNOS ) . -
pubmed
L - NAME abrogated the beneficial effect of
eNOS overexpression . -
pubmed
The mass and heat transfer dynamics of evaporating multicomponent
alcohol / water droplets have been probed experimentally by examining changes in the near surface droplet composition and average droplet temperature using cavity - enhanced Raman scattering ( CERS ) and laser - induced fluorescence (
LIF ) . -
pubmed
Since both events might be linked to the inhibition of
K + channels , we studied whether in vivo application of
minoxidil , a stimulator of
ATP - gated K channels ( K+ ATP channel activator ) prevents both effects . -
pubmed
This twelve - week randomized , double - masked , placebo - controlled study assessed the efficacy and tolerability of the specific and potent oral
dipeptidyl peptidase-4 inhibitor ,
vildagliptin ( 25 mg , bid , n=70 ) VS . -
pubmed
The between - group differences in corrected
insulin response at peak
glucose and mean prandial
C - peptide were + 0.06 + /- 0.02 ( p=0.0258 ) and + 0.10 + /- 0.03 nmol / l ( p=0.0031 ) , respectively . -
pubmed
We have previously shown that
cysteine - affecting mutations could lead to partial endoplasmic reticulum (
ER ) retention . -
pubmed
Treatment of primary oviductal cells with 10 pg / ml
17beta - estradiol or 10 ng / ml
progesterone resulted in a higher expression of
COX-2 and
cPLA ( 2 ) alpha , but not of the other enzymes . -
pubmed
The dissimilatory reduction of 1mmol / L
AQDS can couple to the production of enough
ATP to support cell growth about 60 generations ; The oxidization of electron donors was coupled to the reduction of humus , as reduced humus increased corresponding with increasing of electron donor ; The typical inhibitors such as
Cu2 + which inhibited
Fe - S center ,
Stigmatellin which was
methyl - naphthoquinone model ,
Dicumarol which inhibited oxidized
methyl - naphthoquinone transform to reduced one ,
Metyrapone which was specific inhibitor for
P450 enzyme blocked the humus respiration seriously . -
pubmed
Is the effect of
testosterone on body composition modulated by the
androgen receptor gene CAG repeat polymorphism in elderly men ? -
pubmed
OBJECTIVE : The
androgen receptor (
AR ) gene contains a CAG repeat polymorphism coding for a
polyglutamine chain , the length of which is inversely correlated with
AR transcriptional activity . -
pubmed
Here , we identify a highly conserved 9
amino acid motif in the ligand binding domains ( E domains ) of
human / mouse ER alpha and
ER beta ,
progesterone receptors A and B , and the
androgen receptor . -
pubmed
Mutation of the
phenylalanine or
tyrosine at position-2 ,
cysteine at position 0 , and hydrophobic
isoleucine /
leucine or
leucine /
leucine combinations at positions +5/6 , relative to
cysteine , significantly reduced membrane localization ,
MAP and
PI 3 - kinase activation ,
thymidine incorporation into DNA , and cell viability , stimulated by specific SR ligands . -
pubmed
The localization sequence mediated palmitoylation of each
SR , which facilitated
caveolin-1 association , subsequent membrane localization , and
steroid signaling . -
pubmed
RESULTS : ( 1 ) Compared with that at 0 min , the mean fluorescence intensity of
JC-1 within the spermatozoal body and the percentage of orange - red colored spermatozoa in the experiment group were increased significantly at 5 and 15 min respectively after
uPA incubation ( P & lt ; 0.05 ) . -
pubmed
[ Sequential acupuncture method for treatment of
apoplexy hemiplegia and its effects on plasma
ET-1 and
NO levels ] . -
pubmed
After treatment for 3 months , their clinical therapeutic effects were observed and plasma
endothelin-1 (
ET-1 ) and
nitric oxide (
NO ) levels were detected . -
pubmed
RESULTS : The treatment group in the improvement of the nerve function - deficiency , total life ability and clinical overall effect , and the course of recovery , decreasing of
ET-1 level and increasing of
NO level was superior to the control group ( P & lt ; 0.05 , P & lt ; 0.01 ) , the shorter the course , the better the therapeutic effect . -
pubmed
CONCLUSION : The sequential acupuncture method has an obvious therapeutic effect on
apoplexy hemiplegia , and it can decrease plasma
ET-1 level and increase
NO level . -
pubmed
Mutation of the nuclear factor - kappaB ( NF - kappaB ) binding site significantly impaired both
DON- and LPS - induced
luciferase expression . -
pubmed
In contrast , mutating the
activator protein-1 binding site resulted in significantly increased
DON- and LPS - induced
luciferase expression . -
pubmed
CCAAT / enhancer - binding protein beta ,
octamer-1 , or
NF - kappaB repressing factor binding site mutations did not affect
DON - induced
luciferase activity . -
pubmed
Consistent with reporter studies , the
NF - kappaB inhibitor
caffeic acid phenethyl ester completely ablated both
DON - induced IL-8 mRNA and protein expression . -
pubmed
When
NF - kappaB subunit binding to a specific IL-8 promoter probe was evaluated by enzyme - linked immunosorbent assay ( ELISA ) ,
DON was observed to increase
p65 binding by 21 - fold , have no effect on
p50 binding and decrease
p52 binding . -
pubmed
Taken together , these data suggest that
DON - induced
IL-8 expression is likely to be mediated at the transcriptional level by
NF - kappaB , specifically
p65 , but does not appear to involve mRNA stabilization . -
pubmed
Cardiac microdialysis technique demonstrated that topical perfusion of
acetylcholine increased dialysate
TIMP-1 protein level , which was suppressed by coperfusion of
atropine . -
pubmed
Immunohistochemistry demonstrated a strong expression of
TIMP-1 protein in cardiomyocytes around the dialysis probe used to perfuse
acetylcholine . -
pubmed
Inhibition of
nitric oxide production by the
carbazole compound
LCY-2 - CHO via blockade of
activator protein-1 and
CCAAT / enhancer - binding protein activation in microglia . -
pubmed
LCY-2 - CHO did not block DNA - binding activity of
nuclear factor - kappaB (
NF - kappaB ) or
cyclic AMP response element - binding protein (
CREB ) , but abolished that of
activator protein-1 (
AP-1 ) ,
CCAAT / enhancer - binding protein (
C / EBP ) and
nuclear factor IL6 (
NF - IL6 ) . -
pubmed
LCY-2 - CHO attenuated the nuclear levels of
c - Jun and
C / EBPbeta , but not those of
p65 ,
p50 ,
C / EBPdelta ,
signal transducer and activator of transcription-1 (
STAT-1 ) or the nuclear expression of
IFN regulatory factor-1 (
IRF-1 ) . -
pubmed
Taken together , these results suggest that
LCY-2 - CHO inhibits
NO production in microglia through the blockade of
AP-1 and
C / EBP activation . -
pubmed
Our multiple regression model for DNA tail moment showed that age , high pesticide exposure , low pesticide exposure ,
GSTP1 Ile - Ile , and
XRCC1 399
Arg - Arg genotype were associated with increased DNA tail moment ( DNA damage ) . -
pubmed
Further analysis of interaction between
GSTP1 and
XRCC1 genes that increase susceptibility revealed a significant difference in DNA tail moment for high pesticide - exposed subjects carrying both
GSTP1 Ile - Ile with
XRCC1 399
Arg - Arg genotypes ( 2.49+/-0.09 microm / cell ; P=0.004 ) , compared to those carrying
GSTP1 Ile -
Val /
Val - Val with
XRCC1 399
Arg -
Gln /
Gln -
Gln genotypes ( 1.98+/-0.15 microm / cell ) . -
pubmed
Pectolinarigenin strongly inhibited
COX-2 - mediated
PGE2 and
5 - LOX - mediated
LT production at & gt;1 microM , indicating that it is a dual inhibitor of
COX-2 / 5 - LOX . -
pubmed
However ,
pectolinarigenin did not affect
COX-2 expression or
nuclear transcription factor (
NF - kappaB ) activation . -
pubmed
Endothelial barrier protection by
FTY720 under
hyperglycemic condition : involvement of
focal adhesion kinase ,
small GTPases , and adherens junction proteins . -
pubmed
The phosphorylated form of
FTY720 binds to
S1P receptors to exert
S1P - like biological effects , suggesting endothelial barrier promotion by
FTY720 . -
pubmed
Similar to
S1P , site - specific
FAK tyrosine phosphorylation analysis revealed
FTY720 - induced
FAK [ Y576 ] phosphorylation without phosphorylation of
FAK [ Y397 / Y925 ] . -
pubmed
Furthermore ,
FTY720 conditioned the phosphorylation profile of
FAK [ Y397 / Y576 / Y925 ] in
hyperglycemic medium to the same pattern observed in normoglycemic medium . -
pubmed
FTY720 challenge resulted in small GTPase
Rac activation under hyperglycemic conditions , whereas increased
Rho activity in
hyperglycemic medium was restored to the basal level . -
pubmed
Rac protein depletion by small interfering RNA ( siRNA ) technique completely abolished
FTY720 - induced
FAK [ Y576 ] phosphorylation . -
pubmed
These findings strongly suggest the barrier protective effect of
FTY720 on HMVEC monolayers in
hyperglycemic medium via
S1P signaling , further implying the possibility of
FTY720 as a therapeutic agent of
diabetic vascular disorder . -
pubmed
After release from their precursor
kininogens ,
kinins or their C - terminal
des - Arg metabolites activate two distinct
G protein - coupled receptors (
GPCR ) , called
B2 ( B2R ) or B1 (
B1R ) . -
pubmed
Both receptors can couple to
Galphai and
Galphaq families of
G proteins to release mediators such as
nitric oxide (
NO ) ,
arachidonic acid ,
prostaglandins ,
leukotrienes and endothelium - derived hyperpolarizing factor and can induce the release of other inflammatory agents . -
pubmed
NrdH is one of the intracellular
redoxins exist in Mycobacterium and other microorganisms lacking the common
glutathione system . -
pubmed
NrdH seems a chimeric protein of
glutaredoxin amino acid sequence and
thioredoxin activity . -
pubmed
It serves as a
hydrogen donor for
ribonucleotide reductase . -
pubmed
Results led to a call for more modest treatment goals ( initiation of
insulin at a blood
glucose & gt;180 mg / dl with a goal of approximately 150 mg / dl ) . -
pubmed
To this end , we treated bone marrow - derived
MSC with
estradiol and
progesterone ,
bone morphogenetic protein 2 (
BMP2 ) , and activators of the
protein kinase A (
PKA ) pathway and investigated specific markers of hESF differentiation ( decidualization ) . -
pubmed
Furthermore , we investigated the transcriptome of these cells in response to
cAMP and compared this to the transcriptome of hESF decidualized in response to activation of the
PKA pathway . -
pubmed
Bioactive glasses (
BG ) which contain
strontium have the potential to combine the known bone regenerative properties of
BG with the anabolic and anti - catabolic effects of
strontium cations . -
pubmed
Here we created a
BG series (
SiO ( 2 )- P ( 2 ) O ( 5 )- Na ( 2 ) O - CaO ) in which 0 - 100 % of the
calcium was substituted by
strontium and tested their effects on osteoblasts and osteoclasts in vitro . -
pubmed
They also inhibit osteoclast activity by both reducing
tartrate resistant acid phosphatase activity and inhibiting resorption of
calcium phosphate films in a dose - dependent manner . -
pubmed
Additionally , osteoblasts cultured in contact with
BG show increased proliferation and
alkaline phosphatase activity with increasing
strontium substitution , while osteoclasts adopt typical resorption morphologies . -
pubmed
These effects , when combined with the advantages of
BG such as controlled ion release and delivery versatility , may make
strontium - substituted
BG an effective biomaterial choice for a range of bone regeneration therapies . -
pubmed
When
taxol was added , treated cells arrested indicating that microtubule turnover was necessary for
kinetochore fragment separation . -
pubmed
BACKGROUND /
AIM :
9 -[ 2 -( phosphonomethoxy ) ethyl ] guanine (
PMEG ) is a
guanine acyclic nucleotide analog whose targeted prodrugs are being investigated for chemotherapy of
lymphomas . -
pubmed
The objective of this study was to determine the requirements for
caspase and
CD95 /
Fas activation in
PMEG - induced apoptosis . -
pubmed
Additionally , the influence of
PMEG on
cell cycle regulatory proteins was explored . -
pubmed
Indole and
aliphatic glucosinolates accumulated in response to K - deficiency in a manner that was respectively dependent or independent on signaling through
Coronatine - Insensitive 1 (
COI1 ) . -
pubmed
Amrubicin at a lower - dose with routine prophylactic use of
granulocyte - colony stimulating factor for
relapsed small - cell lung cancer . -
pubmed
To overcome this clinical problem , we investigated whether 35 mg / m(2 ) of
AMR administration with routine prophylactic use of
granulocyte - colony stimulating factor (
G - CSF ) can reduce
myelosuppression , while maintaining efficacy . -
pubmed
CO reduced
acute tubular necrosis , apoptosis ,
tissue factor expression and
P - selectin expression and enhanced proliferative repair as measured by phosphorylation of
retinol binding protein and
histone H3 . -
pubmed
Gene microarray analyses with confirmatory PCR of biopsy specimens showed that
CO blocked proinflammatory gene expression of
MCP-1 and
heat shock proteins . -
pubmed
Complete ophthalmic examination including visual acuity , fundus biomicroscopy , fundus photography ,
fluorescein angiography , and spectral domain optical coherence tomography ( Spectralis
SD - OCT ; Heidelberg Engineering , Heidelberg , Germany ) was performed at baseline and follow - up ( 1 week , 1 month , and 3 months ) , and tolerability of the implant was assessed . -
pubmed
During protein synthesis ,
translation elongation factor Tu (
Ef - Tu ) is responsible for the selection and binding of the cognate
aminoacyl -
tRNA to the acceptor site on the ribosome . -
pubmed
The activity of
Ef - Tu is dependent on its interaction with
GTP . -
pubmed
tuberculosis
Ef - Tu (
MtbEf - Tu ) by
PknB reduced its interaction with
GTP , suggesting a concomitant reduction in the level of protein synthesis . -
pubmed
MtbEf - Tu was found to be phosphorylated by
PknB on multiple sites , including
Thr118 , which is required for optimal activity of the protein . -
pubmed
We found that
kirromycin , an
Ef - Tu - specific antibiotic , had a significant effect on the
nucleotide binding of unphosphorylated
MtbEf - Tu but not on the phosphorylated protein . -
pubmed
Our results show that the modulation of the
MtbEf - Tu -
GTP interaction by phosphorylation can have an impact on cellular protein synthesis and growth . -
pubmed
15 ( S )- HETE at concentration 0.1 muM stimulated proliferation of PASMCs and induced
ERK 1 /
ERK 2 phosphorylation but had no effect on
p38 kinase expression as assessed by Western blotting . -
pubmed
15 ( S )- HETE - stimulated PASMC proliferation was blocked by the
MEK inhibitors
PD-98059 . -
pubmed
Hypoxia ( 3 %
O ( 2 ))-stimulated PASMC proliferation was blocked by
U0126 , a
MEK inhibitor , as well as by
NDGA and
CDC , inhibitors of
15 - LOX , but not by the
p38 MAPK inhibitor
SB-202190 . -
pubmed
Glucagon - like peptide-1 (
GLP-1 ) and
glucose - dependent insulinotropic polypeptide (
GIP ) are incretins produced in the intestine that play a central role in
glucose metabolism and
insulin secretion . -
pubmed
Inhibition of chylomicron formation with
L-81 reduced
GLP-1 secretion into lymph compared to Liposyn stimulation alone ( P = 0.034 ) . -
pubmed
The effect of
L-81 relative to Liposyn alone had an even greater effect on
GIP secretion , which was completely abolished ( P = 0.004 ) . -
pubmed
These findings of a dramatic effect of
L-81 on lymph levels of
GLP-1 and
GIP support a strong link between intestinal lipid absorption and incretin secretion . -
pubmed
The relative difference in the effect of
L-81 on the two incretins provides further support that nutrient - stimulation of
GIP and
GLP-1 is via distinct mechanisms . -
pubmed
Lipopolysaccharide /
adenosine triphosphate - mediated signal transduction in the regulation of
NLRP3 protein expression and
caspase-1 - mediated
interleukin-1beta secretion . -
pubmed
OBJECTIVE : Reactive
oxygen species ( ROS ) plays a critical role in the regulation of
NLRP3 inflammasome activation . -
pubmed
ROS was scavenged by
N - acetyl cysteine ;
NADPH oxidase , the major source of ROS , was inhibited by
diphenyliodonium ,
apocynin or
gp91 - phox siRNA transfection ; and
protein kinase was inhibited by its specific inhibitor . -
pubmed
RESULTS : LPS - induced
NLRP3 protein expression was regulated through the
NADPH oxidase / ROS /
NF - kappaB - dependent ,
JAK2 /
PI3 - kinase /
AKT /
NF - kappaB - dependent , and
MAPK - dependent pathways , while
ATP - induced
caspase-1 activation was regulated through the
NADPH oxidase / ROS - dependent pathway . -
pubmed
CONCLUSIONS : These results demonstrate that ROS regulates not only the priming stage , but also the activation stage , of
NLRP3 inflammasome activation in LPS +
ATP - activated macrophages . -
pubmed
Mutagenesis of the
CPXV203 /
MHCI interface identified the importance of two CPXV203
His residues that confer low pH stabilization of the complex and are critical to
ER retrieval of
MHCI . -
pubmed
The first step of the NETotic cascade is stimulation of one or several receptors followed by activation of the
Raf /
MEK /
ERK pathway that culminates in the assembly of the multimeric
NADPH oxidase complex and the production of reactive
oxygen species ( ROS ) . -
pubmed
Of these 25 , a single protein ,
hydroxysteroid dehydrogenase like 2 , showed decreased expression in both males and females following
arsenic exposure . -
pubmed
We found that the cells expressing
BmAsns protein were capable of influencing the formation of autophagic vacuoles stimulated by
amino acid deprivation . -
pubmed
Altogether , we concluded that the up - regulated expression of
BmASNS would be able to protect cells from impairment induced by
amino acid deprivation , which in turn facilitates cell growth and survival . -
pubmed
Phosphorylation of the activation loop
tyrosine 823 in
c - Kit is crucial for cell survival and proliferation . -
pubmed
Regulation of the catalytic activity of several
kinases is known to be governed by phosphorylation of
tyrosine residues in the activation loop of the kinase domain . -
pubmed
However , in the case of
c - Kit phosphorylation of
Tyr-823 has been demonstrated to be a late event that is not required for
kinase activation . -
pubmed
By using a
tyrosine - to -
phenylalanine mutant of tyrosine 823 , we investigated the impact of
Tyr-823 on
c - Kit signaling . -
pubmed
We demonstrate here that
Tyr-823 is crucial for cell survival and proliferation and that mutation of
Tyr-823 to
phenylalanine leads to decreased sustained phosphorylation and ubiquitination of
c - Kit as compared with the wild - type receptor . -
pubmed
Thus , we propose that activation loop
tyrosine 823 is crucial for activation of both the
MAPK and
PI3 K pathways and that its disruption leads to a destabilization of the
c - Kit receptor and decreased survival of cells . -
pubmed
Structure activity relationship studies of
17 - cyclopropylmethyl-3,14beta - dihydroxy-4,5alpha - epoxy-6alpha -( isoquinoline-3 '- carboxamido ) morphinan (
NAQ ) analogues as potent
opioid receptor ligands : preliminary results on the role of electronic characteristics for affinity and function . -
pubmed
17 - Cyclopropylmethyl-3,14beta - dihydroxy-4,5alpha - epoxy-6alpha -( isoquinoline-3 '- carboxamido ) morphinan (
NAQ ) was previously designed following the ' message - address ' concept and was identified as a potent and highly selective
mu opioid receptor (
MOR ) ligand based on its pharmacological profile . -
pubmed
For the new ligands synthesized as
NAQ analogues , their binding assay results showed that a longer spacer and a saturated ring system of the side chain were unfavorable for their
MOR selectivity over the
kappa and delta opioid receptors . -
pubmed
In contrast , substitutions with different electronic properties at either 1'- or 4'-position of the
isoquinoline ring of the side chain were generally acceptable for reasonable
MOR selectivity . -
pubmed
The majority of
NAQ analogues retained low efficacy at the
MOR compared to
NAQ in the
( 35 ) S - GTP [ gammaS ] binding assays while electron - withdrawing groups at 1'-position of the
isoquinoline ring induced higher
MOR stimulation than electron - donating groups did . -
pubmed
In summary , the electronic characteristics of substituents at 1'- or 4'-position of the
isoquinoline ring in NAQ seem to be critical and need to be further tuned up to achieve higher
MOR selectivity and lower
MOR stimulation . -
pubmed
OBJECTIVE : This study was undertaken in the belief that the atypical antipsychotic drug
quetiapine could prevent apoptosis in the penumbra region following
ischemia , taking into account findings that show
5 - hydroxytryptamine-2 receptor blockers can prevent apoptosis . -
pubmed
The D - I group was administered
quetiapine following
ischemia and sacrificed on the 1st day while the D -
II group was administered
quetiapine every day following the
ischemia and sacrificed on the 3rd day . -
pubmed
ROC analysis reported
Met levels and DNA
MET % , respectively , with a slight and moderate discriminative power . -
pubmed
The degree to which
PBA was bonded to chitosan was related to the
4 - FPBA load used in the reaction ( termed F1 through to
F6 with increasing
4 - FPBA load ) . -
pubmed
Topographic analysis by SEM revealed that
PBA - chitosan conjugates F1 ,
F2 and
F3 have porous matrices and their sensitivity to
glucose adsorption was directly proportional to the degree of
PBA substitution onto chitosan . -
pubmed
Conversely , conjugates F4 ,
F5 and
F6 appeared crystalline under SEM and
glucose adsorption sensitivity decreased in proportion to amount of
PBA bonded to chitosan . -
pubmed
Nephrotoxicity was induced in Groups I -
IV on the 20th day by the administration of a single dose of
cisplatin solution ( 7 mg / kg
body weight , i.p . ) -
pubmed
TA not only prevented the development of
retinal vascular lesions but also inhibited
fluorescein leakage from established
vascular lesions . -
pubmed
This targeting to transcribed sequences requires
SETD2 - mediated methylation of
lysine 36 on histone
H3 and a functional PWWP domain of
DNMT3B. -
pubmed
RESULTS : The maximum concentration of
alpha - terpineol ( 833.93 mg L(-1 ) ) was obtained when the pre - culture medium was in medium log - phase by adding 840 mg L(-1 ) substrate dissolved in
ethanol and cultivation was performed at 24 C , 150 rpm , and pH 6.0 for 12 h. Addition of small amounts of
( R )-(+)- limonene ( 84 mg L(-1 ) ) at the start of fungal log - phase growth yielded a 1.5 - fold yield of
alpha - terpineol , indicating that the
enzyme was inducible . -
pubmed
Although basal
insulin secretion was remarkably high in the isolated pancreatic islets , the responses to both
glucose stimulation and the incretin GLP-1 were retained . -
pubmed
In the pancreatic islets , the
insulin secretory response to
glucose stimulation was maintained but the response to the incretin was diminished . -
pubmed
Ammonium transporters (
AMTs ) are plasma membrane proteins that exclusively transport
ammonium /
ammonia . -
pubmed
Expression analyses showed that 14
AMT genes were vegetative organs expressed ;
AMT1 ; 1 / 1 ; 3 / 1 ; 6 / 3 ; 2 and
AMT1 ; 1 / 1 ; 2 / 2 ; 2 / 3 ; 1 had high transcript accumulation level in the leaves and roots , respectively and strongly changes under the
nitrogen - dependent experiments . -
pubmed
The results imply the functional roles of
AMT genes in
ammonium absorption in poplar . -
pubmed
Re : Ion Channel
TRPM8 Promotes Hypoxic Growth of
Prostate Cancer Cells via an
O2 - Independent and
RACK1 - Mediated Mechanism of
HIF-1alpha Stabilization . -
pubmed
In this study , we analyze the effects of the dual kinase inhibitor
PI-103 , which blocks
PI3 K and
mTOR activity , on three matched pairs of
GBM stem cells / differentiated cells . -
pubmed
While blocking
PI3K - mediated signaling concurrently with application of chemotherapy does not appear to be a valid treatment option , pharmacological inhibitors , such as
PI-103 , nevertheless have an important place in future therapeutic approaches . -
pubmed
The responses to
lenalidomide seem to be inversely correlated to the pre - treatment
EPO level . -
pubmed
This system utilized one -
pot nanocomposite entrapping
Fe3O4 magnetic nanoparticles ( MNPs ) as
peroxidase mimetics and
glucose oxidase (
GOx)/
cholesterol oxidase (
ChOx ) in mesoporous
silica to detect
glucose and
cholesterol in blood samples . -
pubmed
The sensing mechanism involves the generation of
H2O2 by the catalytic action of an immobilized
oxidase on the target molecules in the sample . -
pubmed
The regulatory role of
interferon - gamma producing gamma delta T cells via the suppression of T helper 17 cell activity in
bleomycin - induced
pulmonary fibrosis . -
pubmed
Our aim was to evaluate the neuroprotective effects of
guanosine against oxidative damage in hippocampal slices subjected to an in vitro
ischemia model , the
oxygen /
glucose deprivation (
OGD ) protocol . -
pubmed
We also assessed the participation of
nitric oxide synthase (
NOS ) enzymes activity on the neuroprotection promoted by
guanosine . -
pubmed
Here , we showed that
guanosine prevented the increase in ROS ,
nitric oxide , and
peroxynitrite production induced by
OGD . -
pubmed
Moreover ,
guanosine prevented the loss of mitochondrial membrane potential in hippocampal slices subjected to
OGD . -
pubmed
The protective effects of
guanosine were mimicked by inhibition of
neuronal NOS , but not of inducible
NOS . -
pubmed
The neuroprotective effect of
guanosine may involve activation of cellular mechanisms that prevent the increase in
nitric oxide production , possibly via
neuronal NOS . -
pubmed
In further experiments ,
vitamin B12 - dependent
methylmalonyl - CoA mutase and
methionine synthase activities were detected in cell homogenates . -
pubmed
In particular ,
methionine synthase activity was significantly increased following the addition of
vitamin B12 to the medium . -
pubmed
It remains unclear , however , whether
ethanol also increases NAc DA transmission following operant oral self - administration (
SA ) . -
pubmed
As a follow up to these studies , we recently investigated the changes in NAc shell and core DA transmission associated to oral
SA of a 10 %
ethanol solution . -
pubmed
For the purpose of comparison with literature studies utilizing
sucrose +
ethanol solutions , we also investigated the changes in dialysate DA associated to
SA of 20 %
sucrose and 10 %
ethanol + 20 %
sucrose solutions . -
pubmed
Conversely , under
sucrose SA and extinction DA increased exclusively in the shell . -
pubmed
These observations provide unequivocal evidence that oral
SA of 10 %
ethanol increases dialysate DA in the NAc , and also suggest that stimuli conditioned to
ethanol exposure contribute to the increase of dialysate DA observed in the NAc following
ethanol SA . -
pubmed
Comparison between the pattern of DA changes detected in the NAc subdivisions under
sucrose and
ethanol SA likewise suggests that the NAc shell and core DA play different roles in
sucrose as compared to
ethanol reinforcement . -
pubmed
Lecithin : Cholesterol Acyltransferase Activation by
Sulfhydryl - Reactive Small Molecules : Role of
Cysteine-31 . -
pubmed
Lecithin : cholesterol acyltransferase (
LCAT ) catalyzes plasma
cholesteryl ester formation and is defective in
familial lecithin : cholesterol acyltransferase deficiency (
FLD ) , an
autosomal recessive disorder characterized by low high - density lipoprotein ,
anemia , and
renal disease . -
pubmed
This study aimed to investigate the mechanism by which compound A [
3 -( 5 -( ethylthio )- 1,3,4 - thiadiazol-2 - ylthio ) pyrazine-2 - carbonitrile ] , a small
heterocyclic amine , activates
LCAT . -
pubmed
The site - directed mutation
LCAT -
Cys31Gly prevented activation by compound A. -
pubmed
Substitution of
Cys31 with charged residues (
Glu ,
Arg , and
Lys ) decreased
LCAT activity , whereas bulky hydrophobic groups (
Trp ,
Leu ,
Phe , and
Met ) increased activity up to 3 - fold ( P & lt ; 0.005 ) . -
pubmed
Mass spectrometry of a tryptic digestion of
LCAT incubated with compound A revealed a +103.017 m / z adduct on
Cys31 , consistent with the addition of a single hydrophobic
cyanopyrazine ring . -
pubmed
Finally ,
sulfhydryl - reactive
beta - lactams were developed as a new class of
LCAT activators . -
pubmed
In conclusion , compound A activates
LCAT , including some FLD mutations , by forming a hydrophobic adduct with
Cys31 , thus providing a mechanistic rationale for the design of future
LCAT activators . -
pubmed
Mutation in the conserved
cysteine residue in the NEL domain of
RipAW completely abolished the
E3 ubiquitin ligase activity in vitro and the ability to suppress PTI responses in plant leaves . -
pubmed
Unlike other members of the
IpaH family of effectors ,
RipAW and
RipAR had no
leucine - rich repeat motifs in their
amino acid sequences . -
pubmed
BACKGROUND :
Proprotein convertase subtilisin / kexin type 9 inhibitors ,
Praluent (
alirocumab [ ALI ] ) and Repatha ( evolocumab [ EVO ] ) have been approved as adjuncts to the standard - of - care maximal - tolerated dose ( MTD ) of
low - density lipoprotein cholesterol ( LDLC)-lowering therapy ( LLT ) , statin therapy , in heterozygous ( HeFH ) (
ALI or EVO ) or homozygous ( EVO )
familial hypercholesterolemia , or clinical
atherosclerotic cardiovascular disease (
CVD ) where LDLC lowering is insufficient ( both ) . -
pubmed
SCP was produced by
strain FL023 using corncob hydrolysate and
urea as the
carbon and
nitrogen sources with the dry cell mass productivity 0.86 g L-1 h-1 and the yield of 0.40 g g-1
sugar . -
pubmed
The final skeleton obtained depends on the nature of both the
R2 group of the
allene and the
R3 group of the
allylic residue . -
pubmed
Cholinergic excitation of corticopontine neurons involves at least three ionic mechanisms : suppression of
KV 7 currents , activation of the
calcium - dependent non - specific cation conductance underlying afterdepolarizations , and activation of what appears to be a
calcium - sensitive but
calcium - permeable non - specific cation conductance . -
pubmed
Cholinergic excitation of CPn neurons involved at least three distinct ionic mechanisms : suppression of
KV 7 channels ( the ' M - current ' ) , activation of the
calcium - dependent non - specific cation conductance underlying afterdepolarizations , and activation of what appears to be a
calcium - sensitive but
calcium - permeable non - specific cation conductance . -
pubmed
[ Construction and characterization of
hemolysin S gene mutant strain producing
hyaluronic acid ] . -
pubmed
The yield of
HA , HA molecular weight and virulence factors such as
streptolysin Hylc ,
hyaluronate lyase ,
glyceraldehyde-3 - phosphate dehydrogenase and cell surface proteins were determined by spectrophotometer and
SDS - PAGE . -
pubmed
Berberine Attenuates Macrophages Infiltration in
Intracranial Aneurysms Potentially Through
FAK /
Grp78 /
UPR Axis . -
pubmed
Immunostaining data showed that
BBR inhibited
CD68 + macrophages accumulation in
IAs tissues and suppressed
FAK phosphorylation . -
pubmed
Conclusion :
BBR prevents
IAs formation potentially through inhibiting
FAK phosphorylation and inactivating
UPR pathway in macrophages , which causes less macrophage infiltration and reduced proinflammatory
cytokine release . -
pubmed
The biological indicators of
Qi deficiency related were blood lipids , ECG , blood rheology , inflammatory reaction ,
NO ,
ET and
NF - kappaB signalling pathway . -
pubmed
Systolic (
SBP ) and diastolic (
DBP ) blood pressures were measured using a standard
mercury sphygmomanometer . -
pubmed
Fasting blood
glucose ( FBG ) , lipid profile ,
uric acid and blood
urea nitrogen (
BUN ) , and hs -
CRP were measured using routine methods . -
pubmed
lucidum
alcohol extracts could improve learning and memory function , ameliorate neuronal apoptosis and
brain atrophy , and down - regulate the expression of the
AD intracellular marker ,
Abeta1 - 42 . -
pubmed
We found a subset of
MDR inhibitors also induced IEC delamination , implicating MDR efflux pumps as cellular targets underlying
Glafenine - induced
enteropathy . -
pubmed
Optimization of extraction conditions by a central composite experimental design revealed that the dilution of the
alcohol content of 5 mL of Baijiu to 10 % , followed by the addition of 1.5 g of
NaCl and subsequent SPME Arrow extraction with
DVB /
CAR /
PDMS fiber coating over 45 min at 45 C was the most suitable . -
pubmed
Expression of
SPH kinases ( SPHK ) 1 and 2 , which splice
SPH into
S1P , was also inhibited by
MAT treatment . -
pubmed
In vitro studies showed a direct inhibitory effect of
MAT on
S1P1 expression of activated astrocytes , suggesting that
MAT could function as an
S1PRs antagonist . -
pubmed
Moreover ,
MAT upregulated the expression of plasma
gelsolin , which combines with
S1P to reduce its concentration . -
pubmed
Interestingly , FMT from SHR -
losartan to SHR reduced BP , improved the aortic endothelium - dependent relaxation to
acetylcholine and reduced
NADPH oxidase activity . -
pubmed
Cetirizine is a second - generation antihistamine , derived from the metabolism of
hydroxyzine , highly specific for the
H1 receptors , and with marked antiallergic properties . -
pubmed
HLA - C * 06:284 differs from
HLA - C * 06:02:01:02 by two single nucleotide substitutions in codon 24 (
Ser >
Thr ) . -
pubmed
The branched - chain
amino acids (
BCAA ) play an important role in muscle energy metabolism , and
Kruppel - like factor 15 (
KLF15 ) is an essential regulator of
BCAA metabolism in muscle under
nutritional deficiency . -
pubmed
It can significantly increase the proportion of
G2 / M phase ( up to 3.2 - fold enhancement ) to reactivate
cisplatin via high level of
G2 / M arrest induced by
valproate . -
pubmed
Meanwhile , the intracellular ROS -
P53 crosstalk can be upregulated by
diallyl disulfide ( up to 8 - fold increase of ROS ) and
valproate ( up to 18 - fold increase of
P53 ) to enhance early apoptosis . -
pubmed
The synchronization of enhanced
G2 / M arrest and ROS -
P53 crosstalk devotes to reverse the
cisplatin resistance with a high level of resistance reversion index ( 50.22 ) . -
pubmed
Determinants of Maternal
Renin -
Angiotensin -
Aldosterone - System Activation in Early Pregnancy : Insights From Two Cohorts . -
pubmed
It may thus contribute to the
renin -
angiotensin -
aldosterone - system ( RAAS ) activation that is required for maternal adaptation in pregnancy . -
pubmed
METHODS : Quantification of maternal
prorenin ,
renin and
aldosterone blood levels at 5 , 9 and 11 weeks of gestation . -
pubmed
RESULTS :
Prorenin and
renin were lower in the absence of a CL at all - time points when compared to 1 CL , while
prorenin ,
renin and
aldosterone were higher in the presence of > 1 CL versus 1 CL ( P<0.05 ) . -
pubmed
Ovarian stimulation with menopausal
gonadotropin resulted in higher
prorenin ,
renin and
aldosterone concentrations during late first trimester than recombinant
follicle - stimulating hormone ( P<0.05 ) . -
pubmed
Prorenin , and to a lesser degree
renin , correlated positively with serum
progesterone and
relaxin , but not serum
estradiol . -
pubmed
AIM AND OBJECTIVE : Production of light
olefins from
methanol was studied over
SAPO-34 molecular sieves exploring the effect of mono and dual templates . -
pubmed
A novel homozygous variant in
REN in a family presenting with classic features of disorders involving the
renin -
angiotensin pathway , without
renal tubular dysgenesis . -
pubmed
Autosomal recessively inherited pathogenic variants in genes associated with the
renin -
angiotensin -
aldosterone system (
RAAS ) result in early onset
oligohydramnios and clinical features of the Potter sequence , typically in association with
proximal renal tubules dysgenesis . -
pubmed
Carbon capture and storage (
CCS ) technologies in shale reservoirs have attracted increasing interest in recent years . -
pubmed
In this study , the characteristics of the
CO2 adsorption in shale and its relationship with the shale nano - structure were studied , which may be helpful for understanding
CCS technologies and their application in enhanced shale gas recovery . -
pubmed
( 1972 ) Biochemistry 10 , 2606 - -2617 ) a
protein ( radioactive band 4 ) which migrated slightly slower than
PAS-3 in
SDS gel was labeled heavily by the metal chelate . -
pubmed
This
protein has an apparent molecular weight of 37,500 in 8.4 %
acrylamide -
SDS gel . -
pubmed
The
diazo linkage of the metal chelate to this
protein was found to be especially unstable to heat . -
pubmed
Brain renin activity was partially purified by
ammonium sulfate fractionation and Sephadex gel filtration , resulting in the removal of
angiotensinase activity . -
pubmed
Both enzyme activities were irreversibly inhibited by the
pepsin inhibitor
pepstatin , in nanomolar concentrations . -
pubmed
Interaction of
muscle pyruvate kinase with
1,8 - anilinonaphthalene sulfonate . -
pubmed
Adrenalectomy had a small but significant effect on
kainate binding in the stratum lucidum and stratum radiatum of
CA3 in the first experiment , but no effect in follow - up experiments . -
pubmed
However , little is known about how much each type of cell ,
oxytocin ( OT ) or
vasopressin (
VP ) , contributes to this plasticity during
dehydration . -
pubmed
A method is described for determination of the relative availability of
transferrin - bound
iron and cell - derived
iron to microbial
iron - scavenging mechanisms . -
pubmed
In one culture the
transferrin was labelled with
59Fe and in the other the cells were labelled , and the relative uptake of
radioiron by the microorganisms determined . -
pubmed
coli was observed , suggesting that differences in the ability of aerobactin and
enterochelin to remove
iron from intracellular
ferritin were not responsible for this preference . -
pubmed
Inhibition of
epidermal growth factor - induced activation of
phospholipase C by
psi - tectorigenin . -
pubmed
Ovarian response in repetitive cycles induced by
menotrophin alone or combined with
gonadotrophin releasing hormone analogue . -
pubmed
PHA and
PPD induced stimulations of DNA synthesis and
interleukin-2 (
Il-2 ) production and NK cell activity were measured from peripheral blood mononuclear cells cultured with and without
isoprinosine . -
pubmed
Rabbit Fc - specific antibodies were conjugated with
PNC by a single step
glutaraldehyde bridge . -
pubmed
The
PNC conjugate could be used at a greater dilution than those of the
ALP and
HRP conjugates and the BTB substrate mixture was stable for at least 3 weeks at 4 degrees C.
Penicillin is readily available in developing countries , and at a substantially lower cost than
p - nitrophenyl phosphate , the commonly used substrate for
ALP in the plate ELISA . -
pubmed
Compared with those not so affected ,
hirsutism was associated with a higher mean serum
testosterone concentration ,
infertility was associated with higher mean
gonadotrophin concentrations ,
obesity was associated with a higher mean serum
testosterone concentration ,
hyperprolactinaemia was associated with a lower mean serum
testosterone concentration and smaller ovaries ,
alopecia was associated with lower mean
serum LH and
testosterone concentrations , and
acanthosis nigricans was associated with
obesity and a raised mean serum
testosterone concentration . -
pubmed
The object of this study was to further explore the suppression of pulsatile
LH release by EOPs on day 8 of pregnancy by examining whether inhibition of
norepinephrine ( NE ) or
epinephrine (
EPIN ) synthesis , or stimulation of
gamma - aminobutyric acid ( GABA )- B receptors , modified the ability of
naloxone infusion ( 0.5 mg / kg / hr for 3.5 hr ) to stimulate pulsatile
LH secretion . -
pubmed
1 )
LY 134046 (
PNMT inhibitor , 50 mg / kg
IP ) , given at -27 , -20 , and -3 hr relative to the onset of a 3 - hr blood sampling period , produced no change in hypothalamic - preoptic area ( HPOA ) levels of NE , and a 76 % decline in
HPOA - EPIN levels , as determined by HPLC . -
pubmed
Although basal
LH pulse frequency was reduced , this treatment had no effect on the stimulatory action of
naloxone on pulsatile
LH release . -
pubmed
2 )
FLA-63 (
DBH inhibitor , 25 mg / kg
IP ) given at -3 hr produced a 72 % decline in
HPOA - NE levels , a 44 % decrease in
HPOA - EPIN values , and blocked the stimulatory action of
naloxone on
LH pulse frequency . -
pubmed
Since depletion of
HPOA - EPIN by
LY 134046 did not compromise the
LH response to
naloxone , this effect of
FLA-63 is due to depletion of
HPOA - NE levels . -
pubmed
3 ) While saline had no effect on the increased pulsatile
LH release caused by
naloxone , administration of
baclofen (
GABA - B receptor agonist , 6 mg / kg
IV ) suppressed the pulsatile
LH secretory response to
naloxone infusion. -
pubmed
Synthesis and biological evaluation of
phosphonamidate peptide inhibitors of
enkephalinase and
angiotensin - converting enzyme . -
pubmed
3.4.24.11 ) and
angiotensin - converting enzyme (
ACE , 3.4.14.1 ) has been explored with a series of
enkephalin analogues in which the scissile
Gly3 - Phe4 amide bond has been replaced with a
phosphonamidate moiety . -
pubmed
Within a series of
[ ( N - acylamino ) methyl ] phosphonamidates there was a dramatic decrease in inhibitory activity against
enkephalinase as the
N - acyl moiety was substituted with larger , more hydrophobic
acyl groups . -
pubmed
Likewise , the inhibitory activity of the
[ ( N - acylamino ) methyl ] phosphonamidates against
ACE was attenuated by larger
phenylalkyl acyl functionalities , although not to the same degree as against
enkephalinase . -
pubmed
Over a 2 1/2 - year period , treatment with
melphalan and
prednisolone produced improvement in the
neuropathy and resolution of dermato - endocrine features with a corresponding decline in the
serum paraprotein concentration . -
pubmed
The effect of
3 - aminobenzamide (
3AB ) , an inhibitor of
poly ( ADP - ribose ) synthetase on the frequency of X - ray - induced
chromosomal aberrations in these 3 cell lines was also studied . -
pubmed
3AB potentiated the frequency of aberrations in
G1 and
G2 in all the cell types . -
pubmed
Vitamin - dependent enzyme patterns (
B1 , B2 , B6 ) and excretion of the
tryptophan metabolites kynurenin ,
xanthurenic acid ,
trigonellinamide , and
N - methylpyridone ] . -
pubmed
[
Tinidazol (
CP , 12 - 574 ) in the treatment of
vaginal trichomoniasis ] . -
pubmed
These data suggest that adult and fetal intestinal
alkaline phosphatases represent structurally distinct proteins , either encoded for by different genes or produced by differential processing of a common precursor
molecule determined by a single gene . -
pubmed
The behavioral disruption associated with this manipulation suggests a role for endogenous
LHRH in maintaining sex behavior in a low
testosterone environment . -
pubmed
2OHE2 - 17 beta and 4 - hydroxyestrone , which are potent
estrogens , may act in this instance first as
estrogen agonists , advancing the " time window " when their
catechol antagonist properties in blocking the
LH surge can be functional . -
pubmed
The nonuterotropic
2 - hydroxyestradiol-17 alpha and
2 - hydroxyestrone act solely as
catechol estrogens and inhibit the preovulatory
LH surge if administered at a time when they be present during this physiological window , which is thought to involve events at the
estrogen - catecholaminergic interphase . -
pubmed
Following administration of
bovine growth hormone (
bGH ) and
thyroxine ( T4 ) during the first 20 d of postnatal life , this retarded neuronal growth was restored to normal : neuronal perikarya were enlarged , and the dentrites were thicker with denser branchings . -
pubmed
Primary
autoimmune hypothyroidism was confirmed by the finding of a low serum
thyroxine concentration and a high
thyrotropin concentration . -
pubmed
Sucrose density - gradient ultracentrifugation studies suggest that the
mRF- and
C1q - reactive substances in both
bullous pemphigoid sera and
blister fluids are of a size compatible with immune complexes . -
pubmed
Functional studies of two new abnormal
hemoglobins with their mutation located at intersubunit contacts : Hb Hotel Dieu beta99 (
G1 ) Asp replaced by
Gly and Hb Pitie Salpetriere beta34 ( B16 )
Val replaced by
Phe . -
pubmed
The functional properties of two new abnormal
hemoglobins with high
oxygen affinity were studied . -
pubmed
Hb Hotel Dieu beta99 (
G1 ) Asp replaced by
Gly is situated in the alpha1beta2 contact . -
pubmed
Both
hemoglobins exhibited similar functional properties with a 10 - fold increased
oxygen affinity , a decreased cooperativity , a decreased Bohr effect and a normal or slightly decreased
2,3 - diphosphoglycerate effect . -
pubmed
Similar effects were observed in
ovalbumin - sensitized guinea - pigs upon aerosol of
acetaldehyde ( 20 mg / ml ) which has been administered at the flow rate of 0.1 ml / min for 2 min . -
pubmed
Theophylline (
CAS 58 - 55 - 9 ) antagonized both the increase of ITP values and the rise of
histamine in the blood caused by
acetaldehyde given intravenously ( ED50 = 5.8 mg / kg i.v . ) -
pubmed
or of the
substance P ( SP ) receptor antagonist ,
[ D - Pro4 , D - Trp7.9 ] SP 4 - 11 ( 10 mg / kg i.v . ) -
pubmed
SRF MADS - box position 1 was always a
glycine in the variants , but many different sequences at the other nonconserved MADS - box residues were compatible with efficient DNA binding . -
pubmed
Additionally , the relative abundance of ligand and receptor in the
hIL5.shIL5R alpha complex was determined to be 1:1 by both titration calorimetry and
SDS - polyacrylamide gel electrophoresis analysis of dissolved cocrystals of the complex . -
pubmed
Measurements of the level of control ( LC ) are available such as
glycohemoglobin (
GH ) and
fructosamine (
FA ) . -
pubmed
In comparison with subjects with good LC , patients with poor LC had higher values of duration of
DM ( p & lt ; 0.01 ) , waist / hip ratio ( p & lt ; 0.04 ) , fasting
glucose ( p & lt ; 0.001 ) and lower values of fasting
insulin ( p & lt ; 0.07 ) ,
LDL cholesterol ( p & lt ; 0.006 ) and
VLDL cholesterol ( p & lt ; 0.09). -
pubmed
[ The effect of
wheat germ agglutinin on
anion transport in the erythrocyte membranes ] . -
pubmed
Glycophorin is thought to be associated with
Band 3 , the
anion transport protein , via the cytoskeleton proteins
Band 4.1 ,
spectrin and
ankyrin . -
pubmed
The effect of
WGA on the
anion transport across the erythrocyte membranes was measured by
NO2- transport and
NH4 Cl isotonic swelling , two different methods developed by our laboratory in recent years . -
pubmed
All effects that
WGA exerted above were instantly abolished by adding
N - acetylglucosamine which has specific binding sites on
WGA . -
pubmed
In conclusion , the binding of
WGA to the
sialic acid groups of
glycophorin can lead to the conformational changes in
saccharides and such changes will be transferred via glycophorin , cytoskeleton to
Band 3 , which causes the final change of
anion transport . -
pubmed
These data indicate that endogenous
adenosine inhibits
renin release during
hemorrhagic hypotension , but does not attenuate noradrenergic neurotransmission even during prolonged
hemorrhagic hypotension . -
pubmed
Cells incubated with
insulin produced 5 - 10 fold more
estradiol in response to
IGF - II than those incubated without . -
pubmed
In contrast ,
insulin produced only a small increase of
estradiol in response to
IGF - I. -
pubmed
Effects were reversible either by removal of the
oligomers or by addition of exogenous
IL-8 protein . -
pubmed
Intravenous bolus administration of 125 mg / kg of
cysteamine induced increases in
serum gastrin concentration ( 864 + /- 96 pg / ml ) and gastric acid outputs ( 107 + /- 27 mumol H+/kg.30 min ) not significantly different from the
gastrin 17 - l infusion experiments . -
pubmed
Immunohistochemistry with antibodies directed against
histamine or
histidine decarboxylase revealed that hippocampal transplants received the most dense histaminergic innervation . -
pubmed
Furthermore , surface iodination , immunoprecipitation and
SDS / PAGE analysis were used to identify two
annexin I - binding proteins on the surface of monocytes with molecular masses of 15 kDa and 18 kDa respectively . -
pubmed
5 - HT3 receptor antagonists such as
ondansetron seem to be efficacious both in the control of acute and prolonged
emesis following
carboplatin chemotherapy , but randomly controlled data comparing
ondansetron with other anti - emetic regimens have not yet been published . -
pubmed
Plasma
thyroid hormone concentrations were significantly ( P & lt ; 0.05 ) lower in WR lambs , but there were no differences between
WR and
CR groups in thyroid gland weight or hepatic
iodothyronine 5 '- deiodinase activity at 8 or 29 days of age . -
pubmed
On incorporation of intracellular membrane vesicles containing the delta exon5
CFTR proteins into the lipid bilayer membrane , functional phosphorylation- and
ATP - dependent
chloride channels were identified . -
pubmed
More frequently , the delta exon5
CFTR formed
chloride channels with lower conductance states of approximately 2 - 3 and approximately 4 - 6 pS. -
pubmed
The data obtained indicate that deleting 30
amino acids from the first intracellular loop of
CFTR affects both processing and function of the
CFTR chloride channel . -
pubmed
We describe a reverse transcription
polymerase chain reaction ( RT - PCR ) technique using fluorescent
dUTP to examine changes in mRNA level in samples . -
pubmed
Testosterone treatment of females caused irregular inhibitory effects on the
FHG22 mRNA levels , whereas male castration originated transitory increases during less than 2 weeks . -
pubmed
Treatment of 15 day - castrated males for 1 or 2 days with
beta - estradiol-3 - benzoate caused a marked stimulation in the
FHG22 mRNA levels . -
pubmed
In the absence of hormones , the
FHG22 mRNA levels decreased along the time and neither
progesterone ,
testosterone , or
5 alpha - dihydrotestosterone affected the expression . -
pubmed
However ,
estradiol stimulated the
FHG22 mRNA expression in a time and dose - dependent manner : increasing effects were detected between 8 - 96 h of treatment and the EC50 was about 10(-9 ) M. The
estradiol effect was reverted by the antiestrogen
ICI 164,384 or by
cycloheximide . -
pubmed
We conclude that
estradiol stimulates
FHG22 mRNA expression in Harderian gland , although other agents may also control the expression in vivo . -
pubmed
The induction of
prostaglandin G / H synthase (
PGHS ;
prostaglandin endoperoxide synthase ,
cyclooxygenase ) by proinflammatory
cytokines accounts , at least in part , for the altered
eicosanoid biosynthesis in
inflammatory diseases . -
pubmed
The enhanced production of
PGE2 reflected the upregulation of
PGHS-2 as indicated by enhanced expression of PGHS-2 RNA and increased recovery of
PGHS-2 protein in NHBECs . -
pubmed
When the binding between the
epidermal growth factor ( EGF ) receptor and its ligands was disrupted by a neutralizing antibody (
LA-1 ) ,
IFN - gamma failed to upregulate the release of
PGE2 and the expression of PGHS-2 RNA in NHBECs . -
pubmed
Furthermore ,
IFN - gamma induced the expression of RNAs for a number of ligands at the
EGF receptor TGF - alpha ;
heparin - binding EGF - like growth factor (
HB - EGF ) ; and
amphiregulin in NHBECs , and when administered exogenously , these ligands increased
PGE2 release from NHBECs . -
pubmed
These data are consistent with the presence of an autocrine growth factor /
EGF receptor loop regulating
PGHS-2 expression and
PGE2 synthesis in bronchial epithelial cells . -
pubmed
Induction of
calreticulin expression in response to
amino acid deprivation in Chinese hamster ovary cells . -
pubmed
We characterize here the induction of
calreticulin in response to complete
amino acid deprivation in Chinese hamster ovary cells . -
pubmed
Amino acid deprivation caused a 4 - fold increase in
calreticulin protein levels over a period of 4 - 10 h. In addition to an overall increase in protein levels , the glycosylation of
calreticulin was increased . -
pubmed
This glycosylation event was blocked by
tunicamycin and was not required for the increase in
calreticulin protein levels . -
pubmed
These results are similar to the large increases in
asparagine synthetase mRNA observed in response to
amino acid deprivation , but the
amino acid - deprivation - response element identified to be involved in
asparagine synthetase induction is absent from the
calreticulin promoter . -
pubmed
The release of
AMB from the fatty base of
Witepsol or a medium chain
triglyceride (
MCT ) was markedly faster than that from the hydrophilic base of macrogol . -
pubmed
The addition of
POE ( 2 ) LE into the
MCT base resulted in a marked increase in bioavailability , showing the highest bioavailability of 4.9 % . -
pubmed
Solid state NMR studies of
hydrogen bonding in a
citrate synthase inhibitor complex . -
pubmed
The ionization state and
hydrogen bonding environment of the transition state analogue (
TSA ) inhibitor ,
carboxymethyldethia coenzyme A (
CMX ) , bound to
citrate synthase have been investigated using solid state NMR . -
pubmed
This
enzyme - inhibitor complex has been studied in connection with the postulated contribution of short
hydrogen bonds to binding energies and
enzyme catalysis : the X - ray crystal structure of this complex revealed an unusually short
hydrogen bond between the
carboxylate group of the inhibitor and an
aspartic acid side chain [ Usher et al . -
pubmed
To further investigate the nature of this short
hydrogen bond , low spinning speed 13C NMR spectra of the
CMX - citrate synthase complex were obtained under a variety of sample conditions . -
pubmed
Thus the tensor values for the
enzyme - inhibitor complex could be consistent with either a very long syn
hydrogen bond or an anti
hydrogen bond ; the latter would agree very well with previous crystallographic results . -
pubmed
This proton resonance is assigned to Hdelta2 of
Asp 375 , on the basis of comparison with crystal structures and the fact that this cross - peak was absent in the heteronuclear correlation spectrum of the inhibitor - D375 G mutant
enzyme complex . -
pubmed
The cellular redox status is controlled primarily by
glutathione , a major cellular antioxidant , whose synthesis is regulated by the rate - limiting enzyme
gamma - glutamylcysteine synthetase (
gamma - GCS ) . -
pubmed
gamma - GCS overexpression also completely suppressed
NF - kappa B activation induced by
phorbol ester and
okadaic acid , whereas that induced by
H2O2 ,
ceramide , and lipopolysaccharide was minimally affected . -
pubmed
Overall , our results indicate that most of the pleiotropic actions of
TNF are regulated by the
glutathione - controlled redox status of the cell . -
pubmed
The
enzyme released
acetate from several acetylated substrates , including acetylated xylan . -
pubmed
Using
p - nitrophenyl acetate as a substrate , the
enzyme had a K(m ) of 0.9 mM and a V(max ) of 785 micromol min(-1 ) mg(-1 ) . -
pubmed
ADP - ribosylation factor ( ARF ) 6 regulates plasma membrane trafficking and cortical
actin formation by cycling between inactive
GDP and active
GTP - bound conformations . -
pubmed
We also describe experiments that propose a central role in this pathway for the
PI 3 - kinase - dependent plasma membrane recruitment of the
cytohesin-1 family of
PtdIns ( 3,4,5 ) P ( 3 )-binding
ARF - exchange factors . -
pubmed
Agonists (
isoproterenol or
nitroprusside ) were used to stimulate
adenylyl cyclase and
guanylyl cyclase , respectively , in the absence and presence of nonselective
IBMX or
PDE5 specific inhibitors
E4021 ( 1 ) . -
pubmed
The
PDE activities reside predominantly in the supernatant , but the
PDE activity for degrading
cGMP is more pronounced . -
pubmed
Moreover , results with
E4021 suggest that
PDE5 activity could play a critical role in modulating
cGMP - related activity in the trabecular meshwork . -
pubmed
An accumulation of extracellular
lactate was observed after inhibition of the
lactate transporters suggesting that transport of
lactate into neurones is necessary for the neuroprotective effect of
17beta - estradiol . -
pubmed
These results suggest a new neuroprotective mechanism of
17beta - estradiol by activating
glutamate - stimulated
lactate production , which is
estrogen receptor - dependent . -
pubmed
Our results show that a single intraventricular administration of
HIVgp120 selectively increases REMS and that such an increase can be prevented by
U0126 , an inhibitor of
ERK activating enzyme ,
MEK . -
pubmed
In contrast ,
SB202190 , a
MAPK -
p38 inhibitor , had no effect on
HIVgp120 - induced increase in REMS . -
pubmed
We have previously reported a single
nucleotide polymorphism ( SNP ) at nucleotide ( nt ) position -88 ( G or T ) within an interferon - stimulated response element - like sequence in the promoter region of the
MxA gene , which correlated with responsiveness of
hepatitis C patients to
interferon . -
pubmed
The case of the
platelet glycoprotein IIb / IIIa inhibitor ,
abciximab , is used to illustrate many of the issues surrounding the collection , interpretation , and ( mis ) application of cost - effectiveness data . -
pubmed
The neuronal
serine protease inhibitor neuroserpin is widely expressed in the developing and adult brain . -
pubmed
Measurement of multiple psi torsion angles in uniformly
13C,15N - labeled
alpha - spectrin SH3 domain using 3D
15N-13C-13C-15N MAS dipolar - chemical shift correlation spectroscopy . -
pubmed
We demonstrate the simultaneous measurement of several backbone torsion angles psi in the uniformly
( 13 ) C ,( 15 ) N - labeled
alpha - Spectrin SH3 domain using two different 3D 15N-13C-13C-15N dipolar - chemical shift magic - angle spinning ( MAS ) NMR experiments . -
pubmed
BACKGROUND :
Interleukin ( IL )- 18 has been shown to activate basophils to produce
histamine and
IL-4 and to induce naive T cells to differentiate into T - helper ( Th ) 2 cells . -
pubmed
The performance of the nonadentate chelate was compared with a
heptadentate chelate in a dry reagent immunoassay for
human chorionic gonadotropin (
hCG ) . -
pubmed
Contrary to the
heptadentate label , the effect of aqueous quenching on the nonadentate label was found to be insignificant , with calculated analytical detection limits ( background + 3 SD ) of 0.9 and 0.7 IU / L
hCG for wet and dry measurements , respectively , and a linear range up to 5000 IU / L. The CVs for the new label were & lt;8 % at the cutoff of 25 IU / L and above in both whole blood and plasma . -
pubmed
Copper sensitized astrocytes to the action of
menadione , an intracellular generator of
superoxide anion radical , exogenous
hydrogen peroxide (
H2O2 ) and
rotenone , an inhibitor of
mitochondrial electron transport chain complex I. -
pubmed
Four electrode materials :
Pt ,
Au ,
Pd and
glassy carbon (
GC ) , were studied to investigate their suitability as substrates in the development of two different classes of
glutamate biosensor . -
pubmed
Glutamate oxidase cross - linked onto
poly ( o - phenylenediamine ) was chosen as the type 1 biosensor (
PPD /
GluOx ) , incorporating
PPD as the permselective element to detect
H ( 2 ) O ( 2 ) directly on the electrode surface at relatively high applied potentials . -
pubmed
GluOx and
horseradish peroxidase / redox polymer modified electrodes (
Os ( 2 +) PVP / HRP /
GluOx ) that relied on
enzyme - catalysed
H ( 2 ) O ( 2 ) detection at lower applied potentials were used as type 2 biosensors . -
pubmed
The voltammetric and amperometric responses to the
enzyme signal transduction molecule ,
H ( 2 ) O ( 2 ) , and the archetypal interference species in biological applications ,
ascorbic acid , were determined on the bare and
PPD /
GluOx - modified surfaces . -
pubmed
Cadherins are
Ca ++-dependent cell - cell adhesion molecules which play an important role in the cellular connection between normal cells . -
pubmed
cynocephalus ) were autotransfused with RBCs that had been harvested using
CPD and that had been resuspended and stored in AS-1
solution at 4 degrees C for as long as 49 days . -
pubmed
Phase
II / III trials should be done to compare
docetaxel with liposomal
anthracyclines as a first - line treatment . -
pubmed
Niemann - Pick disease type C (
NPC ) is a
genetic disorder in which patient cells exhibit lysosomal accumulation of
cholesterol and
sphingolipids ( SLs ) caused by defects in either
NPC1 or
NPC2 proteins . -
pubmed
The clearance of lysosomal free
cholesterol was also associated with a decrease in
LDL - receptor levels . -
pubmed
Reduction of
naphthoquinones by
DT - diaphorase is often described as a detoxification reaction . -
pubmed
In the present study , the effect of several substances that are known to increase tissue activities of
DT - diaphorase on the
toxicity of
2 - amino-1,4 - naphthoquinone has been investigated . -
pubmed
Again like
2 - hydroxy-1,4 - naphthoquinone , the severity of the
haemolysis induced by the
2 - amino derivative was increased in animals pre - treated with inducers of
DT - diaphorase , but the degree of
nephrotoxicity was decreased . -
pubmed
With these substances , therefore ,
DT - diaphorase both activates and detoxifies the
quinone , depending on the target organ . -
pubmed
It is not possible to generalize with regard to the effects of modulation of tissue levels of
DT - diaphorase on
naphthoquinone toxicity in vivo , since this may change not only the severity of the toxic effects , but also the target organ specificity . -
pubmed
These clusters include one cluster (
Mal I , PF1737 to PF1751 ) involved in
maltose metabolism and another cluster ( PF0691 to PF0695 ) whose products are thought to remove toxic reactive
nitrogen species . -
pubmed
The results obtained demonstrate that
MC - NO : ( a ) absorbs in the UVB region even after UVA irradiation ; ( b ) acts as free radical scavenger as demonstrated by
EPR experiments ; ( c ) strongly reduces both UVA- , sunlight- and
BMDBM - induced lipid peroxidation in liposomes , measured as reduced TBARS levels ; and ( d ) has comparable antioxidant activity to that of commonly used
vitamin E and
BHT in skin care formulations . -
pubmed
Ca2 + sparks are the elementary units of intracellular
Ca2 + signaling in striated muscle cells revealed as localized
Ca2 + release events from sarcoplasmic reticulum (
SR ) by confocal microscopy . -
pubmed
Adsorption , ionization , and migration of
hydrogen chloride on ice films at temperatures between 100 and 140 K. Adsorption of
hydrogen chloride (
HCl ) on water ice films is studied in the temperature range of 100 - 140 K by using
Cs+ reactive ion scattering ( Cs+
RIS ) , low energy sputtering ( LES ) , and temperature - programmed - desorption mass spectrometry ( TPDMS ) . -
pubmed
Falsely low
LDL cholesterol results and
cholestasis . -
pubmed
We have identified two new
histone deacetylase (
HDAC ) inhibitors ( 9 and 24 ) capable of inducing the expression of gamma - globin and/or beta -
globin promoter - driven reporter genes in a synthetic model of Hb switch . -
pubmed
nBAF complexes bind tightly to the
Ca ( 2 +)-responsive dendritic regulator
CREST and directly regulate genes essential for dendritic outgrowth . -
pubmed
Recent work strongly suggests that
NADPH oxidase is a major source of
superoxide in cardiovascular cells , and oxidative stress can be involved in the process of
endothelial dysfunction . -
pubmed
From the viewpoint of these molecular mechanisms ,
HMG - CoA reductase inhibitors ( statins ) might inhibit the high
glucose - induced
NADPH oxidase activation through inhibition of
Rac activity and finally prevent the increase in ROS production in
diabetes . -
pubmed
Beta - blockers decrease liver perfusion and inhibit the activity of hepatic microsomal
lidocaine metabolizing enzymes of the
P450 _ 2D subfamily . -
pubmed
After making a mark on the stromal side of a microkeratome - prepared DSAEK corneal button to confirm appropriate orientation of the donor tissue after insertion into the anterior chamber , the corneal tissue was returned to Optisol
GS solution for 1 hour . -
pubmed
The
glutathione peroxidase (
GPx ) activities of some
diaryl diselenides incorporating
tertiary amino groups were studied with
H ( 2 ) O ( 2 ) ,
Cum - OOH , and
tBuOOH as substrates and with
PhSH as
thiol co - substrate . -
pubmed
Simple replacement of a
hydrogen atom with a
methoxy group dramatically enhances the
GPx activity . -
pubmed
The chemical identities of these components were confirmed by
( 1 ) H NMR and EI -
MS . -
pubmed
Aromatase inactivation by 2 - substituted derivatives of the suicide substrate
androsta-1,4 - diene-3,17 - dione . -
pubmed
To gain the structure - activity relationship of
Delta ( 1 )- androstenediones (
Delta ( 1 )- ADs ) as mechanism - based inactivator of
aromatase , series of
2 - alkyl - and
2 - alkoxy - substituted Delta ( 1 )- ADs ( 6 and 9 ) as well as
2 - bromo - Delta ( 1 )- AD ( 14 ) were synthesized and tested . -
pubmed
All of the
alkyl steroids 6 along with the
alkoxy steroid 9 , except for the
ethyl and
n - propyl compounds 6b and 6c , caused a time - dependent inactivation of
aromatase . -
pubmed
The results indicate that the
2 - hexyl compound 6f act as the most powerful mechanism - based inactivator of
aromatase among
Delta ( 1 )- AD analogs and may be submitted to the preclinical study in
estrogen - dependent
breast cancer . -
pubmed
Due to the presence on histology of lymphoid follicles and the persistent high serum
immunoglobulin E levels , we elected to attempt treatment with
rituximab . -
pubmed
The efficacy of flaxseed oil supplementation was investigated in terms of survival of animals and production of various inflammatory molecules (
malondialdehyde ,
myeloperoxidase ,
nitric oxide ) in the lung homogenate of animals . -
pubmed
This was accompanied by decreased inflammatory markers (
malondialdehyde ,
myeloperoxidase ,
nitric oxide ) as the disease progressed . -
pubmed
Yeast Elongation protein 3 (
yElp3 ) , the catalytic subunit of the multi - subunit
histone acetyltransferase elongator complex , is involved in histone acetylation and transcription , exocytosis and
tRNA modification . -
pubmed
For immunoassay , specificity can be an issue especially in relation to the proportion of
25OHD2 that is quantified whereas HPLC and LC -
MS /
MS methods are able to measure the two major
vitamin D metabolites
25OHD2 and
25OHD3 independently . -
pubmed
The
tyrosine kinase receptor
RET51 is expressed in distinct families of neurons where it promotes different functions . -
pubmed
The substitution of the
tyrosine 905 of
RET51 , a key residue phosphorylated by both
GDNF and
NGF , disrupts the
RET51 /
FKBP52 complex . -
pubmed
Response of
perianal fistulas to antibiotics (
metronidazole alone or combined with
ciprofloxacin ) was significantly higher in patients without
CARD15 mutations ( 7.7 % versus 40.5 % , P = 0.041 ) . -
pubmed
However , significant decreases of plasma and ovarian
inhibin alpha were seen after reinstatement of estrous cyclicity with
progesterone implant treatment . -
pubmed
Investigational approaches discussed include
aminothienopyridazine inhibitors of
tau aggregation , the alternative splicing of
tau pre - mRNA ,
protein phosphatase 2A as a potential therapeutic target , and immunotherapy and macroautophagy approaches for clearing aberrant
tau protein from the brain . -
pubmed
The combined structural and mutagenesis results suggest that during decarboxylation of
ferulic acid by
FADase ,
Trp25 and
Tyr27 are required for the entering and proper orientation of the substrate while
Glu134 and
Asn23 participate in proton transfer . -
pubmed
The aim of the current study was to explore in vitro the effects of
STP on neuronal activity of
GP neurons during local repetitive stimulation . -
pubmed
Nephropathy was defined as
microalbuminuria ( urinary
albumin -
creatinine ratio [ ACR ] : 30 to 299 mg / g ) ,
macroalbuminuria ( urinary
ACR : & gt;= 300 mg / g ) , or an estimated
glomerular filtration rate of & lt;60 mL / min/1.73 m(2 ) . -
pubmed
Ouabain - sensitive and
ouabain - resistant
Rb+
( K+ ) influxes were measured during regulatory cell volume increase ( RVI ) and apoptotic volume decrease ( AVD ) . -
pubmed
Here , we demonstrate that while
replication factor C subunit 1 (
RFC1)-
RFC is required for
Cdt1 degradation after UV irradiation during the
nucleotide excision repair process , another
RFC complex ,
Ctf18 -
RFC , which is known to be involved in the establishment of cohesion , has a key role in
Cdt1 degradation in S phase . -
pubmed
Mitochondrial neurogastrointestinal encephalopathy (
MNGIE ) is caused by
deficiency in thymidine phosphorylase (
TP ) , that regulates
thymidine (
dThd ) and
deoxyuridine (
dUrd ) . -
pubmed
,
IV -
L - glutamine ( 250 mg / kg , p.o . ) -
pubmed
Glycosylated
haemoglobin , lipid profile , plasma and colonic active ( GLP-1 ) ( 7 - 36 )
amide , mRNA expression of
proglucagon GLP-1 , plasma and
pancreatic insulin , histology of pancreata and biomarkers of oxidative stress (
superoxidase dismutase ,
reduced glutathione ,
malondialdehyde ,
glutathione peroxidase ,
glutathione S transferase ) were measured after 8 week . -
pubmed
,
II -
sitagliptin ( 5 mg / kg , p.o . ) -
pubmed
,
IV -
L - glutamine ( 500 mg / kg , p.o . ) -
pubmed
Plasma
glucose , active
GLP-1 ( 7 - 36 )
amide concentration and
insulin levels were measured after
glucose loading . -
pubmed
The docking data indicated that
l - glutamine bind to the
GLP-1 receptor . -
pubmed
In this study we labeled the anti -
human epidermal growth factor receptor type 2 (
HER2 ) Nanobody with
( 68 ) Ga via a
1,4,7 - triazacyclononane-1,4,7 - triacetic acid (
NOTA ) derivative and assessed its use for
HER2 iPET imaging . -
pubmed
Biodistribution studies showed fast and specific uptake ( percentage injected activity [ % IA ] ) in
HER2 - positive
tumors ( 3.13 + - 0.06 and 4.34 + - 0.90 % IA / g for
( 68 ) Ga - NOTA-2Rs15dHis6 and
( 68 ) Ga - NOTA-2Rs15d , respectively , at 1 h after injection ) and high
tumor - to - blood and
tumor - to - muscle ratios at 1 h after injection , resulting in high - contrast PET /
CT images with high specific
tumor uptake . -
pubmed
CONCLUSION : A new anti -
HER2 PET tracer ,
( 68 ) Ga - NOTA-2Rs15d , was synthesized via a rapid procedure under mild conditions . -
pubmed
Up - regulation of urinary markers predict outcome in
IgA nephropathy but their predictive value is influenced by treatment with
steroids and
azathioprine . -
pubmed
OBJECTIVE :
Steroids and immunosuppressants can delay progression of renal function in
IgAN , but their possible effect in local
cytokines has not been studied . -
pubmed
Serum
creatinine at time of diagnosis had significant correlation with
proteinuria ( p = 0.02 ) , urinary levels of
IL-1beta ( p = 0.03 ) ,
IL-2 ( p = 0.01 ) and
MCP-1 ( p = 0.03 ) . -
pubmed
Steroids +
aza may exert their beneficial effect through suppression of the production or activation of most
cytokines . -
pubmed
Under both unstimulated and
RANKL - stimulated conditions ,
Nrf2 loss led to an increase in the intracellular ROS level and the oxidized - to - reduced
glutathione ratio and a defect in the production of numerous antioxidant enzymes and
glutathione . -
pubmed
Moreover , pretreatment with
N - acetylcysteine or
diphenyleneiodonium significantly reduced the OC differentiation and decreased the intracellular ROS level in both
Nrf2 - deficient and wild - type cells . -
pubmed
Pretreatment with
sulforaphane and
curcumin also inhibited the OC differentiation by activating
Nrf2 in part . -
pubmed
RESULTS : In GI and GII , addition of
plerixafor led to a significant ( p & lt ; 0.01 ) increase of leukocytes and
CD34 + cells in peripheral blood (
PB ) . -
pubmed
In GII , the median number of
CD34 + cells in
PB before and after addition of
plerixafor was significantly ( p = 0.019 ) higher compared to GI ( 9 vs. 5 and 50 vs. 24 cells / mul , respectively ) . -
pubmed
CONCLUSIONS :
Plerixafor and
G - CSF mobilization or the addition of
plerixafor during non - optimal chemotherapy and
G - CSF mobilization together with LVL enabled , independent of leukocyte count and even without detectable
CD34 + cells before addition of
plerixafor , sufficient harvest of HPC numbers for transplantation . -
pubmed
Mechanism of action of
colchicine :
IV . -
pubmed
Embryogenesis was induced with three genotypes using a modified half - strength Murashige and Skoog (
MS ) medium supplemented with 1.0 to 4.0 mg l(-1 )
2,4 - dichlorophenoxyacetic acid (
2,4 - D ) and 0.33 to 1.0 mg l(-1 )
kinetin . -
pubmed
Within two to three months after culture initiation , embryos were transferred to modified
MS medium containing 0.2 mg l(-1 )
6 - benzyladenine ( BA ) and 2 %
sucrose and placed in the light for conversion into plantlets . -
pubmed
It is concluded that the
NAA - binding - sites are probably located at the external
face of the plasma membrane ; they are destroyed during protoplast isolation by proteolytic enzymes in the
cellulase and macerozyme preparations . -
pubmed
The mitochondria promote branching through
ATP generation and the determination of localized hot
spots of active axonal mRNA translation , which contribute to
actin - dependent aspects of branching . -
pubmed
Group A - Bioactive glass ( SHY -
NM ) , Group B -
Fluoride tooth paste ( Amflor ) , Group C -
CPP -
ACP ( Tooth mousse ) , Group D -
CPP - ACPF ( Tooth mousse plus ) , Group E - control . -
pubmed
Results showed that the
retinoic acid receptor alpha (
RARalpha ) , a target protein in
acute promyelocytic leukemia (
APL ) , was in the top rank , with a pharmacophore model matching well the molecular features of
Tanshinone IIA . -
pubmed
These results indicated that
RARalpha may be a potential target of
Tanshinone IIA for
APL . -
pubmed
From our nucleosome model , it is evident that substitution of specific
amino acid residues in
TH2B results in both differential histone - DNA and histone - histone contacts . -
pubmed
Recognition of foreign
antigens by T lymphocytes is restricted by molecules of the
major histocompatibility complex (
MHC ) . -
pubmed
The technique of
Texas Red immunostaining for cellular
gammaH2AX identifies by fluorescence microscopy DNA undergoing active remodeling or repair . -
pubmed
Energy metabolism genes encoded proteins primarily involved in
H2 uptake and electron cycling , including a
rhodanese - containing complex that is phylogenetically conserved among
sulfate - reducing Deltaproteobacteria . -
pubmed
Exposure to
1,4 - BQ showed no significant effect on CD3e(+ ) cells but reduced the total counts of
Sca-1(+ ) ,
CD11b(+ ) ,
Gr-1(+ ) , and
CD45(+ ) cells at 7 and 12 muM ( p & lt ; 0.05 ) . -
pubmed
Skeletal muscles continuously produce reactive
oxygen species ( ROS ) and reactive
nitrogen species (
RNS ) at
rest and more so during contraction . -
pubmed
Morphine is a substrate of
P - glycoprotein and the
P - glycoprotein - mediated efflux transport at the BBB maintains a lower unbound concentration of
morphine in the brain compared with plasma . -
pubmed
Unlike EM ,
AFM imaging can be performed under physiological conditions , enabling the real - time visualization of
PFT assembly and the transition from the prepore state , in which the toxin does not span the membrane , to the pore state . -
pubmed
In addition to characterizing
PFT oligomers ,
AFM has also been used to examine toxin - induced alterations in membrane organization . -
pubmed
In this review , we summarize the contributions of
AFM to the understanding of both PFT assembly and
PFT - induced membrane reorganization . -
pubmed
Serum
lactate and base deficit are very sensitive markers for detecting and monitoring the extent of
bleeding and
shock , in conjunction with repeated combined measurements of
prothrombin time , activated partial
thromboplastin time ,
fibrinogen and platelets . -
pubmed
The methylation of SINEs is increased compared with their flanking regions , and the strongest effect is visible for
cytosines in the
CHH context , indicating an involvement of asymmetric methylation in the silencing of SINEs . -
pubmed
The growth inhibitory effect of these
lectins on
pancreatic cancer cells were shown to be a consequence of
lectin cell surface binding and triggering G0 /
G1 arrest , mitochondrial membrane depolarization , sustained increase of the intracellular
calcium release and the apoptotic signal is amplified by activation of
caspases executing cell
death . -
pubmed
Both OS ( hazard ratio ( HR)=1.14 , 95 % CI 0.80 - 1.63 , P=0.448 ) and PFS ( HR=1.20 , 95 % CI 0.95 - 1.52 , P=0.119 ) were similar in the
erlotinib compared to the chemotherapy group using
IPW - adjusted Cox regression analysis treating the use of
erlotinib as a time - dependent covariate starting from second - line treatment and stratified for ECOG performance status and treatment line . -
pubmed
F1F0 ATP synthases are bidirectional molecular motors that translocate protons across the cell membrane by either synthesizing or hydrolyzing
ATP . -
pubmed
Unlike mesophilic
ATP synthases , alkaliphilic enzymes have tightly regulated
ATP hydrolysis activity , which can be relieved in the presence of
lauryldimethylamine oxide . -
pubmed
In addition , epigenetic analysis revealed a differential methylation pattern in
TPO , and
triiodothyronine tissue levels were significantly decreased ( P<0.01 ) . -
pubmed
Adjustment for
E2F1 expression attenuated the association of
ASK1 with fasting
glucose ,
insulin resistance , circulating
IL-6 , and lipids (
triglycerides ,
HDL -
cholesterol ) , even after adjusting for
BMI . -
pubmed
An innovative MWCNTs /
DOX /
TC nanosystem for chemo - photothermal combination therapy of
cancer . -
pubmed
We previously developed an innovative
TAT - chitosan functionalized MWCNTs ( MWCNTs /
TC ) based drug delivery system for
doxorubicin (
DOX ) and preliminarily investigated its release profile and antitumor effect . -
pubmed
In the present study , the application potential of MWCNTs /
DOX /
TC in chemo - photothermal combination therapy was further explored . -
pubmed
The in vivo anti -
tumor effect of MWCNTs /
DOX /
TC was further evaluated by noninvasive bioluminescence imaging . -
pubmed
Using commercially available nanoparticles , continuous wave Surface - Enhanced Stimulated Raman spectroscopy ( CW
SE -
SRS ) is demonstrated for the first time using two
Ti : Sapphire lasers producing a pump beam ( 785 nm , 100 mW ) and appropriately varying probe / Stokes beams ( 860 - 870 nm , 120 mW ) . -
pubmed
Removal of
lead ( II ) from aqeouos waste using (
CD -
PCL -
TiO2 ) bio - nanocomposites . -
pubmed
In this study ,
titanium dioxide was synthesized via sol - gel technique then incorporated in a polymer blend (
CD -
PCL ) via solution blending method . -
pubmed
HS is treated with the
IL-1 Receptor antagonist (
anakinra ) but also specifically with
IL-18BP . -
pubmed
In addition , results showed that the architecture and distribution of grafted
polyNaSS on
Ti surfaces alter the intensity of the bacteria response mediated by
fibronectin . -
pubmed
Interestingly ,
H12 contains a distinct cluster of cis - elements like
dehydration - responsive element ( DRE - core ; GCCGAC ) , an
ABA - responsive element ( ABRE ; ACGTGTC ) and as-1 element ( TGACG ) which are known to be induced by cold , drought and pathogen /
SA respectively . -
pubmed
All WU conditions reduced the energy cost ( EC ) of running , respiratory exchange ratio , and step frequency (
SF ) after the experimental and control phases of WU , while blood
lactate (
BLC ) increased in uphill and level WUs and decreased in control WUs . -
pubmed
This protein exists as a dimer of two identical subunits and contains two intramolecular
disulfide bonds that are responsible for its resistance to
trypsin digestion and allergenicity . -
pubmed
Based on
SDS - PAGE , HPLC analysis , and competitive ELISA , the reduction of
disulfide bonds of
BLG with
OsNTRB /
OsTrx23 ,
OsNTRB /
OsTrx1 , GSH /
OsTrx1 , or
GSH /
OsTrx20 increased its
trypsin digestibility and reduced its immunoreactivity . -
pubmed
In the hypostome ,
HmTSP- and
Wnt3 - expressing cells were identical or in close vicinity to each other , and regions of ectopic tentacle formation induced by pharmacological
beta - Catenin activation (
Alsterpaullone ) corresponded to foci of
HmTSP expression . -
pubmed
TlpA has been implicated in sensing
arginine ,
bicarbonate , and acid , but no experimentally determined protein structures of
TlpA were available to better understand ligand binding and signal transduction . -
pubmed
Comparisons with
TlpC show that despite high similarity across the general structure ,
TlpA lacks the residues required to bind
lactate , and instead contains a pocket almost entirely hydrophobic in nature . -
pubmed
Cytochrome P450 ( Cyp ) 17A1 has both
17alpha - hydroxylase and
17,20 - lyase activities , which are involved in the steroidogenic pathway that produces
androgens and
estrogens . -
pubmed
These results confirm that adequate
estrogen is essential for maintaining ovarian differentiation , and they provide new insight into the role of
FSHbeta in male testicular development and spermatogenesis . -
pubmed
The toxins comprise 73
amino acid residues and 5
disulfide bridges , being homologous to
alpha - bungarotoxin (
alpha - BgTx ) , a classical blocker of muscle - type and neuronal
alpha7 , alpha8 , and alpha9alpha10 nAChRs . -
pubmed
On the other hand , the
hydroxyls groups available on MSNs surface allows the conjugation of specific molecules which can binds to the overexpressed
Enhanced Growth Factor Receptor (
EGFR ) in many
tumors , representing a potential strategy for the
cancer treatment . -
pubmed
Monoamine receptor interaction profiles of
4 - aryl - substituted 2,5 - dimethoxyphenethylamines (
2C - BI derivatives ) . -
pubmed
Many ring - substituted
phenethylamines exert psychedelic effects that are thought to be primarily mediated by interactions with
serotonergic 5 - hydroxytryptamine 2 ( 5 - HT2A ) receptors . -
pubmed
In contrast ,
2C derivatives with bulky lipophilic substituents at the 4 - position of the
phenyl ring tend to display antagonist behavior at
serotonin 5 - HT2 receptor sites . -
pubmed
To gain a better understanding of agonist and antagonist behavior of substituted
phenethylamines , binding affinities and functional activation and inhibition of a series of
4 '- aryl substituted 2,5 - dimethoxyphenethylamine (
2C - BI derivatives ) at various
monoamine receptors were determined . -
pubmed
Additionally ,
2C - BI-8 and
2C - BI-12 activated
serotonin 5 - HT2A and
5 - HT2B receptors at submicromolar concentrations . -
pubmed
2C - BI-1 and 2C - BI-7 were the only
2C - BI derivatives to activate
human trace amine - associated receptor 1 . -
pubmed
2C - BI-3 and
2C - BI-4 interacted with
monoamine transporters but with low overall potency . -
pubmed
The relatively high affinities of various
2C - BI derivatives at the
serotonin 5 - HT2A receptor indicate a high steric tolerance of the binding pocket . -
pubmed
Potent partial activation of the
serotonin 5 - HT2A receptor by
2C - BI-8 and
2C - BI-12 suggests that these substances may potentially exert psychedelic effects similar to other compounds of the
2C family . -
pubmed
The 2 - year RFS and overall survival rates for
DCF versus
ACF were 64.1 % versus 42.9 % ( hazard ratio 0.53 , 95 % confidence interval 0.33 - 0.83 , P = 0.0057 ) and 78.6 % versus 65.4 % ( P = 0.08 ) , respectively . -
pubmed
Degradation of DNA by
DNase 1 was monitored at a steady state by making changes in the fluorescence polarisation of
EtBr in droplets with a volume of 330 picolitre at a 40 hertz frequency under visible light . -
pubmed
Using this technique , we successfully determined the half - maximal inhibitory concentration ( IC50 ) of
ethylenediaminetetraacetic acid (
EDTA ) for the inhibition of
DNase 1 activity to be 1.56 + - 0.91 mM. -
pubmed
We halted autophagy by introducing
Atg 5 specific siRNA or the autophagy inhibitor
Bafilomycin A1 (
Baf - A1 ) . -
pubmed
Treatment with
IKK16 ( 1 mg / kg
body weight ) was administered , by tail vein , 15 min
post - LPS . -
pubmed
At 24 h
post -
IKK16 treatment , serum enzyme levels indicative of liver , kidney , pancreas and muscle function were evaluated by biochemical analysis , and RT - PCR technique was used to analyze gene expression of pro - inflammatory
cytokines . -
pubmed
Moreover , as expected , LPS promoted a significantly overexpression of
TNF - alpha ,
IL-6 and
IL-1beta in the heart , kidney , and liver ; which was diminished by
IKK16 treatment . -
pubmed
However , further studies on cardiac biomarkers and other diagnostic tools in
CO poisoning are needed given the low sensitivity of
troponin I. -
pubmed
METHODS : A well designed single - center cross - sectional study was performed and the
GFR was determined by 3 methods separately in the same day :
technetium-99m - diethylene triamine pentaacetic acid (
99mTc - DTPA ) dual plasma sample clearance method ( mGFR ) ;
FAS equation method ; Asian modified CKD - EPI equation method . -
pubmed
Both the
FAS equation and Asian modified CKD - EPI can be a satisfactory method and may be the most suitable
creatinine - based equation . -
pubmed
Pygenic Acid A (
PA ) Sensitizes Metastatic
Breast Cancer Cells to Anoikis and Inhibits Metastasis In Vivo . -
pubmed
In this process ,
dyslipidemia indexes (
LDL ,
apolipoproteins A ) correlate with the
calcium volume whereas blood
glucose , as measured in the oral
glucose tolerance test , correlates with the lipid component of
coronary plaque . -
pubmed
had a high proportion of potential genes responsible for methanogenesis and
nitrogen fixation , whereas the
CAB of Temora spp . -
pubmed
At the 6th week of intervention ,
glucose contents of intraperitoneal
glucose tolerance test ( IPGTT ) and intraperitoneal
insulin tolerance test ( IPITT ) were measured . -
pubmed
Serum total
cholesterol (
TC ) and total
triglyceride (
TG ) were assayed by using an automatic biochemical analyzer . -
pubmed
hydrolases with particular reference to
pyrophosphate and
orthophosphate monoester phosphohydrolases . -
pubmed
The predominant pathway for
ethanol oxidation at low
ethanol concentrations involves
alcohol dehydrogenase . -
pubmed
Oxidation of
ethanol under these conditions is associated with a change in the steady - stage concentration of
catalase -
H2O2 . -
pubmed
Based on recent evidence , we conclude that it is unnecessary to postulate that
ethanol is oxidized directly via
cytochrome P-450 . -
pubmed
Acetaldehyde production from
ethanol via the microsomal subfraction can be accounted for by the combined activities of
catalase -
H2O2 and
alcohol dehydrogenase . -
pubmed
The metabolism of
ehtanol via
alcohol dehydrogenase produces a marked reduction in the hepatocellular
NAD -
NADH sytems . -
pubmed
Attempts in inhibit
alcohol dehydrogenase with
alkylpyrazoles and activate
catalase with substrates for peroxisomal
H2O2 - generating
flavoproteins , while successful , may have limited applicability because of the native toxicity of the substrates themselves ... -
pubmed
Coexistence of contractile and relaxant
5 - hydroxytryptamine receptors coupled to distinct signaling pathways in intestinal muscle cells : convergence of the pathways on
Ca2 + mobilization . -
pubmed
In naive cells from both regions ,
5 - HT caused contraction and stimulated increases in cytosolic free
calcium concentration ( [
Ca2+]i ) ( 3 - fold ; p & lt ; 0.01 ) and
cAMP levels ( 40 - 60 % ; p & lt ; 0.01 ) that were inhibited , respectively , by the 5 - HT2 antagonist
ketanserin and the 5 -
HT1p antagonist
N - acetyl-5 - hydroxytryptophyl 5 - hydroxytryptophan amide (
5 - HTP - DP ) . -
pubmed
In cells maximally contracted with a non-5 -
HT agonist ( cholecystokinin octapeptide ) ,
5 - HT caused relaxation when the contractile effect mediated by
5 - HT2 receptors was blocked with
ketanserin ; relaxation and the concomitant increase in
cAMP were inhibited by
5 - HTP - DP . -
pubmed
In cells with only
5 - HT2 receptors ,
5 - HT caused contraction and an increase in [
Ca2+]i but not in
cAMP levels ; contraction and the increase in [
Ca2+]i were inhibited by
ketanserin . -
pubmed
Conversely , in cells with only
5 - HT1p receptors ,
5 - HT caused relaxation and an increase in
cAMP levels but not in [
Ca2+]i ; relaxation and the increase in
cAMP levels were inhibited by
5 - HTP - DP . -
pubmed
Thus , the increase in [
Ca2+]i was augmented 1 ) when
cAMP production was inhibited by
5 - HTP - DP in naive cells or 2 ) when
cAMP production was suppressed in cells where
5 - HT1p receptors were inactivated and only
5 - HT2 receptors were preserved . -
pubmed
The results imply that the increase in
cAMP levels mediated by
5 - HT1p receptors acted to attenuate the increase in [
Ca2 +]i mediated by
5 - HT2 receptors . -
pubmed
alpha - hANP was biotinylated using
N - hydroxysuccinimidobiotin and trapped onto an anti - alpha -
hANP [ 6 - 28 ]
IgG - coated
polystyrene ball . -
pubmed
The complex formed was trapped onto (
anti-2,4 - dinitrophenyl group )
IgG - coated
polystyrene balls and , after washing , reacted with
avidin -
beta - D - galactosidase conjugate . -
pubmed
The
polystyrene balls were washed , and the complex of the three components was eluted with
epsilon N-2 ,
4 - dinitrophenyl - L - lysine and transferred to anti -
fluorescein IgG - coated
polystyrene balls . -
pubmed
beta - D - Galactosidase activity bound to the last
polystyrene balls was assayed by fluorometry . -
pubmed
Only 12 - 26 % of
[ 3H ] thymidine influx occurred through this transporter , the remainder entering the cells through a
thymine / uracil - specific transporter described previously . -
pubmed
However ,
NADH formation as a result of
hydrogenase interaction with anion - radicals (
CO2.- ) formed in the course of
formate photooxidation can not at present be excluded . -
pubmed
Peripheral blood lymphocytes taken during hospitalization with
IAR therapy ( first half and latter half ) , and every 3 to 6 months for 2 years at the longest after
IAR therapy were double - stained with
FITC- and
PI - labelled antibodies and two - color analysis was conducted by a FACS Analyzer . -
pubmed
Thus we conclude that
RP 67580 , a
SP antagonist , belongs to a class of drugs that may be useful in the management of various clinical pathologies where
pain and
neurogenic inflammation are involved . -
pubmed
The
pentapeptide ,
Tyr - Ile - Gly - Ser - Arg , ( YIGSR ) , an
amino acid sequence from the B1 chain of
laminin , was found to compete with
laminin for binding to the
laminin receptor . -
pubmed
Progesterone receptor was measured in 97
breast cancer biopsy samples with two methods , the multiple point dextran coated
charcoal method with Scatchard analysis and enzyme immunoassay . -
pubmed
One obvious advantage with
enzyme immunoassay compared to the dextran coated
charcoal method is that significantly less tissue is needed . -
pubmed
Arterial
thromboxane B2 and
platelet factor 4 concentrations were elevated on cardiopulmonary bypass in all groups . -
pubmed
Whereas 1 microgram / ml of
compound 48 / 80 slightly stimulated
Ca2 + -dependent
phospholipase A2 , higher concentrations led to dose - dependent inhibition of this platelet enzyme . -
pubmed
These results demonstrate that the inhibition of
platelet aggregation by
compound 48 / 80 is not due solely to effects on
calmodulin as previously reported , but that inhibition of
phospholipases and probably
arachidonate mobilization may also be involved . -
pubmed
Urine
IR - EGF (
U - EGF ) increased about 100 - fold relative to
urea concentration and about 1000 - fold relative to urine volume from the 1st day of life to adulthood . -
pubmed
The participation of extracellular
calcium in transmission of activation into the cell being excluded , the
Ca2 + -activation of the contractile apparatus and kinase - phosphorylase system in electrically stimulated muscles presumably proceeds from the induction of
Ca2 + release from sarcoplasmic reticulum (
SR ) by transmembrane
Na +- or
Li+-current . -
pubmed
The mechanism of inhibition of
phosphoenolpyruvate carboxylase by
quinolinic acid . -
pubmed
Following the C - banding procedure it was possible to differentially stain the centromeric area with Feulgen and
GCA and even with non - fully specific stain for DNA such as
methylene blue . -
pubmed
Lysine residues in the C - terminal lobe and lysosomal targeting of
procathepsin D. -
pubmed
A major pathway to the lysosome for soluble
hydrolases involves the 6 - phosphorylation of
mannose residues . -
pubmed
BCECF labeling of cells indicated that the parent and variant cells possess an
amiloride sensitive
Na +/ H + antiporter of comparable activity . -
pubmed
Exposure of parent cells to
PGF2 alpha or
TPA resulted in the activation of
Na +/ H + antiporter activity . -
pubmed
These studies strongly suggest that
PKC mediated activation of the
Na +/ H + antiporter accounts for the inhibition of
ammonia production produced by both
PGF2 alpha and
TPA . -
pubmed
Interleukin 1 beta converting enzyme (
ICE ) is responsible for processing an inactive 31 - kDa precursor to the active , mature 17 - kDa
Il-1 beta with cleavage occurring between the
Asp116 -
Ala117 amide bond . -
pubmed
Upon cleavage of
DABCYL - Tyr - Val - Ala - Asp - Ala - Pro - Val - EDANS (
DABCYL - ICE - EDANS ) , an increase in fluorescence is observed at the EDANS emission wavelength of 490 nm , permitting a continuous assay of
ICE that is useful in the screening of inhibitory compounds . -
pubmed
A partial prevention of muscle waste was observed with
acetylsalicylic acid , a non - steroidal anti - inflammatory drug , or with
leupeptin , a
proteinase inhibitor . -
pubmed
Although treatment with
insulin decreased plasma
corticosterone to normal values , it did not modify the circulating level of
tumour necrosis factor . -
pubmed
Histone
H4 , at concentrations up to 11.8 microM , increased
2 - deoxyglucose (
2 - DG ) uptake in a dose - dependent fashion . -
pubmed
However , at concentrations higher than 11.8 microM ,
H4 caused a decrease in
2 - DG uptake from the maximum , suggesting a secondary inhibitory action of this compound . -
pubmed
The maximal effect of
H4 on
2 - DG uptake was not additive to the maximal effect of
insulin . -
pubmed
Moreover ,
2 - DG uptake in the presence of both
H4 and
insulin was significantly lower than the
2 - DG uptake in the presence of
insulin alone . -
pubmed
The maximal effect of
H4 on stimulation of
2 - DG uptake was neither additive nor inhibitory to the maximal effects of the intracellularly acting
insulin mimetics
sodium vanadate or
H2O2 . -
pubmed
Also , in contrast with the effects of
H4 on
insulin - stimulated
2 - DG uptake ,
H4 did not inhibit
insulin - like growth factor - I (
IGF - I)-stimulated
2 - DG uptake , as the maximal effects of
H4 and
IGF - I were additive . -
pubmed
These data suggest that
H4 interacts with the
insulin , rather than the
hypoxia / contraction , pathway for activation of
glucose transport in muscle tissue , and that
H4 acts either directly or indirectly to increase the number of
insulin receptors at the surface of the muscle cell . -
pubmed
In trabeculae with intact endothelial cells ,
cAMP increased
actomyosin ATPase activity toward an apparently maximum value . -
pubmed
In trabeculae with damaged endothelial cells ,
cAMP did not change
actomyosin ATPase activity . -
pubmed
The extent to which the force of the isolated trabecula is changed by the coronary venous effluent is closely related to the degree to which
cAMP has up - regulated the
actomyosin ATPase activity in the isolated heart donating the coronary effluent : the greater the degree of up - regulation of
ATPase activity , the greater the increase in force produced by the effluent . -
pubmed
The rates of desorption of the
NBD - phospholipids from
phosphatidylcholine ( PC ) donor vesicles was in the order
phosphatidylcholine ( PC ) & gt ;
phosphatidylserine (
PS ) & gt ;
phosphatidylethanolamine (
PE ) , the slower rates in the PS and
PE reflecting direct interlipid
hydrogen bonding . -
pubmed
Ethanol also increased the rate of
NBD - phospholipid desorption from donor PC vesicles in the order PC & gt ; PS & gt ; PE , showing that PC interactions , here limited to the weaker
hydrogen - bonded water molecule network , were more susceptible compared to stronger , direct interlipid
hydrogen bonds involving
PE and
PS . -
pubmed
We found that induced sputum from
asthmatic subjects had a higher percentage of eosinophils [ 8.1 + /- 3.43 ( mean + /- SEM ) versus 0.03 + /- 0.02 % , p & lt ; 0.009 ] ( after excluding squamous cells ) and also had higher levels of
albumin ( 232.3 + /- 54.8 versus 79.5 + /- 9.7 micrograms / ml , p & lt ; 0.02 ) ,
fibrinogen ( 44.2 + /- 11.6 versus 11.9 + /- 2.5 micrograms / ml , p & lt ; 0.008 ) and
eosinophil cationic protein (
ECP ) ( 142.6 + /- 34.2 versus 26.1 + /- 4.7 ng / ml , p & lt ; 0.006 ) but not of
histamine or
tryptase . -
pubmed
Characterization of the secondary structure of the antitumor antibiotic
C-1027 has been made from a comparison of C-1027 and its
apoprotein by various analytical means . -
pubmed
The results indicated the antibiotic to be abundant in beta - structure by measurements of Fourier - transform infrared ( FT - IR ) spectroscopy and the circular dichroism (
CD ) spectrum , and by a prediction of the secondary structure based on the
amino acid sequence of the peptide . -
pubmed
In comparison of the IR spectra of their
proteins in
D2O , the
apoprotein exhibited a faster H - D exchange than C-1027 , indicating an increase in the " non - motile parts " of the beta - sheets formed through the
protein - chromophore interaction in
holo - C-1027 . -
pubmed
Partial
amino acid sequence analysis of this protein indicated that these components involved a heterogeneously modified single polypeptide , and that this antigen is identical to
human prostatic acid phosphatase . -
pubmed
Glucokinase (
GK ) plays a key role in the regulation of
glucose - induced
insulin secretion , and questions have been raised about its relationship to the
glucose transporter GLUT2 and its function in
diabetes . -
pubmed
The translocation of this key enzyme for
glucose recognition by beta - cells may lead to altered rates of
insulin secretion during acute perturbations of fuel provision and in the
diabetic state . -
pubmed
A standardized
hyperthyroid symptom score (
HSS ) and thyroid function tests including
thyroid - stimulating hormone (
TSH ) , free
thyroxine ( free T4 ) , free
triiodothyronine ( free T3 ) , total
thyroxine ( T4 ) and total
triiodothyronine ( T3 ) were evaluated before and after 10 and 30 days of the study period . -
pubmed
HSS decreased from 27.80 + /- 4.54 to 22.51 + /- 4.04 after 10 days of
diltiazem therapy in Group 1 ( p & lt ; 0.01 ) . -
pubmed
The
cobalt ( III )-insulin hexamer is a prolonged - acting
insulin prodrug . -
pubmed
The
insulin hexamer has two high - affinity metal ion binding sites , each involving three
HisB10 residues , one from each dimer . -
pubmed
Insulin hexamers containing
Co2 + at both these sites were oxidized to form a stable
Co ( 3 +)-insulin complex . -
pubmed
It is shown that the
Co ( 3 +)-coordinated
insulin monomers are released extremely slowly in aqueous solution at pH 8.0 , and that the hexamer does not spontaneously dissociate into subunits at nanomolar concentrations of
insulin . -
pubmed
The
Co ( 3 +)-insulin hexamer is not recognized by the
insulin receptor in vitro but the complex shows a protracted action profile following subcutaneous ( s.c . ) -
pubmed
The
Co ( 3 +)-insulin hexamer provides a novel prodrug approach to a soluble , prolonged - acting
insulin preparation of potential use for basal
insulin delivery in the treatment of
diabetes . -
pubmed
Hypertension risk increased with age , heart rate , serum
triglycerides ,
insulin area , and
obesity ( in young participants ) . -
pubmed
The 5 ' regulatory region of
ORF1 contains early ( CGTGC ) and late ( TAAG ) transcriptional initiation motifs and
ORF1 is predicted to encode a protein with 114
amino acid residues . -
pubmed
ORF4 is included in ORF6 - part and can encode a 48
amino acid residues polypeptide , but ORF4 and
ORF6 - part are located on different DNA strands . -
pubmed
Dysfunction of
nitric oxide induces
protein kinase C activation resulting in
vasospasm after
subarachnoid hemorrhage . -
pubmed
We hypothesize that the interaction between
protein kinase C (
PKC ) and
nitric oxide (
NO ) plays a role in the modulation of cerebral vascular tone , and the disturbance of this interaction following
subarachnoid hemorrhage (
SAH ) results in
vasospasm . -
pubmed
N omega - nitro - L arginine (
L - NA ) , an inhibitor of
NO production , enhanced
PKC activity . -
pubmed
PKC activity in the
SAH was significantly higher than in the control ; however , no further enhancement was produced with
L - NA . -
pubmed
In the
SAH ,
PKC activity was not inhibited either by
8 - bromo - cGMP or
SIN-1 . -
pubmed
We conclude that
NO maintains an appropriate vascular tone through inactivation of
PKC , and that this effect is disturbed following
SAH , resulting in
PKC - dependent vascular contraction , such as
vasospasm . -
pubmed
On the other hand , once
PKC has been activated ,
NO precursors do not inhibit
PKC . -
pubmed
These facts indicate
NO inactivates
PKC through the inhibition of
phosphatidylinositol breakdown . -
pubmed
ISO , with and without
NP , increased
cyclic AMP ( from 287 + /- 21 to 477 + /- 33 pmol / g ) without altering
cyclic GMP . -
pubmed
Left - ventricular pressure increased from 97 + /- 12 to 178 + /- 9 mm Hg and dP / dtmax from 1,786 + /- 275 to 4,049 + /- 354 mm Hg / s.
NP increased
cyclic GMP ( from 4 to 30 pmol / g ) in both the absence and presence of
ISO , but
NP did not alter
cyclic AMP . -
pubmed
Without
ISO ,
NP insignificantly altered left - ventricular pressure ; however , in the presence of
ISO ,
NP significantly decreased left - ventricular pressure by -25 + /- 4 mm Hg and decreased dP / dtmax by -619 + /- 142 mm Hg / s.
Isoproterenol increased
O2 consumption , but the changes with
NP were not significant . -
pubmed
When this study was repeated in the presence of
LY83583 , a
guanylate cyclase inhibitor ,
NP still produced
cardiac depression in the presence of
ISO . -
pubmed
Therefore , cardiodepressant effects of
NP were only observed against a background of inotropic stimulation with
ISO . -
pubmed
However , effects of
NP on contractility were unrelated to increases in
cyclic GMP or
cyclic GMP - induced changes in
cyclic AMP . -
pubmed
Pretreatment of RAEC with
db - cAMP ( 1 mM ) attenuated
Exo A induced
cytotoxicity . -
pubmed
G protein coupled receptors for
prostaglandins are activated when agonists are bound to a binding pocket formed in part by the seven transmembrane domains . -
pubmed
Recent studies have determined that substitution of a conserved
threonine in the second extracellular loop of the
prostaglandin EP3 receptor resulted in increased affinity for ligands with a
C1 methyl ester moiety . -
pubmed
The homologous
threonine in the second extracellular loop of the
human prostaglandin EP2 and EP4 receptors was mutated to
alanine . -
pubmed
In contrast ,
EP2 and
EP4 receptors bearing conservative
threonine to
serine mutations ( EP2T185S ; EP4T168S ) displayed Kd values for
[ 3H ] prostaglandin E2 similar to wild type receptors : 8.8 + /- 0.7 nM for
EP2T185S compared to 12.9 + /- 1.2 nM for
EP2 wild type ; 2.0 + /- 0.8 nM for
EP4T168S compared to 0.9 + /- 0.3 nM for the
EP4 wild type receptor . -
pubmed
The EC50 values for
cAMP stimulation were 1.3 + /- 0.6 nM for
EP2 wild type ; 2.7 + /- 1.3 nM for
EP2T185S ; 1.1 + /- 0.3 nM for
EP4 wild type ; and 1.4 + /- 0.33 nM for
EP4T168S. -
pubmed
These studies suggest a critical role for the
hydroxyl moiety on these conserved
threonine residues at position 168/185 of the second extracellular loop in
prostaglandin receptor - ligand interactions . -
pubmed
A randomized prospective comparison of oral
levofloxacin plus intraperitoneal (
IP )
vancomycin and
IP netromycin plus
IP vancomycin as primary treatment of
peritonitis complicating
CAPD . -
pubmed
OBJECTIVE : To compare the therapeutic efficacy of daily oral
levofloxacin plus intermittent intraperitoneal (
IP )
vancomycin ( group 1 ) versus daily IP
netromycin and intermittent
IP vancomycin ( group 2 ) in the primary treatment of
peritonitis complicating continuous ambulatory peritoneal dialysis ( CAPD ) . -
pubmed
CONCLUSION : Oral
levofloxacin in combination with intermittent IP
vancomycin has comparable efficacy to
IP netromycin combined with intermittent IP
vancomycin as primary treatment in
CAPD peritonitis , but is simpler and more cost - effective to administer . -
pubmed
Although treatment with
donepezil did not normalize
EP latencies , treatment was associated with a significant decrease in the auditory
P300 latency ( mean latency pretreatment=401 . -
pubmed
Treatment with
donepezil had no discernible effect on auditory or visual
P300 EP amplitudes . -
pubmed
As expected , the particle free energy is higher in the right - handed state ( DeltaG(t)=1.9(+/-0.I ) kT ) , but it decreased ( to 1.3(+/-0.1 ) kT ) upon
histone acetylation and the addition of
phosphate , a potent tail destabilizer . -
pubmed
Eukaryotic translation initiation factor 5A (
eIF-5A ) is the only cell protein that contains the unusual basic
amino acid hypusine [
N epsilon -( 4 - amino-2 - hydroxybutyl ) lysine ] . -
pubmed
Hypusine is formed by the transfer of the
butylamine portion from
spermidine to the
epsilon - amino group of a specific
lysine residue of
eIF-5A precursor and the subsequent hydroxylation at
carbon 2 of the incoming
4 - aminobutyl moiety . -
pubmed
Here we have studied whether the inhibition of
hypusine synthesis can potentiate the anti - cancer activity of the anti - tumour agents
interferon - alpha (
IFN alpha ) and
cytosine arabinoside (
ara - C ) . -
pubmed
Growth inhibition induced by
IFN alpha was paralleled by decreased
hypusine synthesis and , when
EGF counteracted anti - proliferative effects , a reconstitution of
hypusine levels was recorded . -
pubmed
UNLABELLED :
Calcium chloride (
CaCl ( 2 ) ) is ineffective in severe
calcium channel antagonist
overdoses . -
pubmed
Digoxin increases intracellular
calcium by inhibiting the
sodium - potassium adenosine triphosphatase enzymes . -
pubmed
Mutation of
Cys-67 alters the thermodynamic stability of the
human leukocyte antigen HLA0 - B * 2705 . -
pubmed
Among the four heavy chain mutations , the C67S change was investigated for unfolding and
peptide binding properties because this position may mediate
disulfide pair bridging and alter T - cell recognition of
HLA - B * 2705 . -
pubmed
Cys-67 seems to play a crucial role in controlling the thermodynamic stability of the
B * 2705 - peptide complexes as the C67S mutant unfolds faster and with a single transition , independent of pH. -
pubmed
Bradykinin - induced increase in the intracellular concentration of free
calcium evokes an activation of the
endothelial nitric oxide synthase (
eNOS ) enzyme , producing
nitric oxide (
NO ) . -
pubmed
Cigarette smoke inhibits the
eNOS -
NO -
cGMP signaling pathway . -
pubmed
After the purification with
DEAE ion - exchange chromatography ,
HSP70 with a purity of higher than 90 % was obtained . -
pubmed
Activation of
hydrogen peroxide in
horseradish peroxidase occurs within approximately 200 micro s observed by a new freeze - quench device . -
pubmed
To observe the formation process of compound I in
horseradish peroxidase (
HRP ) , we developed a new freeze - quench device with approximately 200 micro s of the mixing - to - freezing time interval and observed the reaction between
HRP and
hydrogen peroxide (
H ( 2 ) O ( 2 ) ) . -
pubmed
We conclude that the activation of
H ( 2 ) O ( 2 ) in HRP at ambient temperature completes within approximately 200 micro s. The developed device can be generally applied to investigate the electronic structures of short - lived intermediates of
metalloenzymes . -
pubmed
Immunohistochemical staining of cells using isoform - specific antibodies further demonstrated translocation of the
phorbol ester - sensitive isoforms ,
PKCalpha and
PKCepsilon , to both the plasma membrane and perinuclear sites , reflecting potential initial steps in regulation of specific effector pathways . -
pubmed
Indeed , selective inhibition of
PKCs indicates a potential role for
PKCalpha in modulating a
calcium influx channel . -
pubmed
The in vitro and in vivo enantioselectivity of
etomidate implicates the
GABAA receptor in general anaesthesia . -
pubmed
In electrophysiological and radioligand binding assays , the enantiomers of
etomidate positively regulated
GABAA receptor function at anaesthetic concentrations and with an enantioselectivity paralleling their in vivo activity . -
pubmed
GABA - evoked currents mediated by
human recombinant GABAA receptors were potentiated by either
R -(+)- or
S -(-)- etomidate in a manner dependent upon receptor subunit composition . -
pubmed
Modulation of
GABA receptor activity was selective ;
R -(+)- etomidate inhibited
nicotinic acetylcholine , or 5 - hydroxytryptamine3 receptor subtypes only at supra - clinical concentrations and
ionotropic glutamate receptor isoforms were essentially unaffected . -
pubmed
Collectively , these observations strongly implicate the
GABAA receptor as a molecular target relevant to the anaesthetic action of
etomidate . -
pubmed
The protein encoded by one of the clones interacting with
neuroglobin (
NGB ) was confirmed to be the C terminus of the
Na (+ ) , K (+)- ATPase beta2 subunit (
NKA1b2 ) based on
amino acid sequences . -
pubmed
We tested whether
dexamethasone (
DEXA ) administration influences the proliferation , apoptosis , and number of B- and T - lymphocytes in Peyer 's patches (
PP ) and thymus in calves fed colostrum ( C ) or a milk - derived formula . -
pubmed
The
DEXA ( 30 microg / kg of BW daily ) was injected for 4 d into groups fed colostrum on the first 3 d ( CD+ ) and those fed the formula that contained nutrients in amounts as in colostrum but no
immunoglubulin G ( FD+ ) . -
pubmed
There were significant effects ( P & lt ; 0.05 ) of
DEXA treatment ( decrease of cell proliferation rates in follicles of
PP and thymus , increase of apoptotic rate in follicles of
PP and thymus , decrease of B - lymphocyte numbers in follicles of
PP , increase of B - lymphocyte numbers in domes of
PP , increase of T - lymphocyte numbers in follicles of
PP , and a decrease of intraepithelial T - lymphocyte numbers ) . -
pubmed
In conclusion ,
DEXA treatment decreased cell proliferation rates in follicles of
PP and thymus and enhanced apoptotic rates in follicles of
PP . -
pubmed
Colostrum feeding decreased cell proliferation rates , likely of B - lymphocytes , in follicles of
PP and numbers of B - lymphocytes in domes , follicular - associated epithelium , and interfollicular areas of
PP and enhanced cell proliferation rates and selectively modified
DEXA effects in the thymus . -
pubmed
THE MEASUREMENT OF FIBRINOLYSIS WITH RADIOACTIVE
MERCURY LABELLED
FIBRIN CLOTS . -
pubmed
[ The treatment of the
Parkinson syndrome with
disipal (
BS 5930 ) ] . -
pubmed
METHODS : We retrospectively evaluated hazardous concomitant prescriptions of
hydroxymethylglutaryl coenzyme A reductase inhibitors ( statins ) and
azole antifungals , which were invoiced through 9 months of 2001 within an outpatient setting . -
pubmed
The euryarchaeal
nitrogen repressor
NrpR controls the expression of the
nif (
nitrogen fixation ) operon , resulting in full repression with
ammonia , intermediate repression with
alanine , and derepression with
dinitrogen . -
pubmed
Here we have undertaken both in vivo and in vitro approaches to study the way in which
NrpR ,
nifOR ( 1 ) ,
nifOR ( 2 ) , and the effector
2 - oxoglutarate (
2OG ) combine to regulate
nif expression , leading to a comprehensive understanding of this archaeal regulatory system . -
pubmed
nifOR ( 1 ) has stronger binding and by itself can mediate the repression of
nif transcription during growth on
ammonia , unlike the weakly binding
nifOR(2 ) . -
pubmed
However ,
nifOR(2 ) in combination with
nifOR(1 ) is critical for intermediate repression during growth on
alanine . -
pubmed
NrpR responds directly to
2OG , which weakens its binding to the operators . -
pubmed
Hence ,
2OG is an intracellular indicator of
nitrogen deficiency and acts as an inducer of
nif transcription via
NrpR. -
pubmed
We studied single -
nucleotide polymorphisms in
transforming growth factor ( TGF )- beta1 ,
interleukin ( IL )- 10 ,
IL-6 ,
interferon ( IFN )- gamma and
tumour necrosis factor ( TNF)-alpha genes in the Finnish population using family - based association approach . -
pubmed
To validate the method , three contrast agents targeted to
integrins were synthesized by grafting to USPIO ( ultrasmall particles of
iron oxide ): ( a ) the
CS1 ( connecting segment-1 ) fragment of
fibronectin (
FN ) ( USPIO - g - FN ) ; ( b ) the peptide GRGD ( USPIO - g - GRGD ) ; ( c ) a non - peptidic RGD mimetic ( USPIO - g - mimRGD ) . -
pubmed
Delta
R2 was converted in values of
iron concentration based on a calibration curve . -
pubmed
Low - density lipoprotein -
cholesterol ,
lipoprotein ( a ) , and
hemoglobinA1C levels were determined . -
pubmed
NM-3 significantly suppressed the increase of
VEGF induced by high
glucose in cultured podocytes and also suppressed the increase of
VEGF and
TGF - beta induced by high
glucose in cultured mesangial cells . -
pubmed
Barth syndrome (
BTHS ) is a
mitochondrial disorder that is caused by mutations in the
tafazzin gene , which affects
phospholipid composition . -
pubmed
G - protein beta-3 subunit C825 T polymorphism ,
sodium and arterial blood pressure : a community - based study of Japanese men and women . -
pubmed
Epidemiological evidence on gene - environment effects of the
G - protein beta-3 subunit C825 T polymorphisms and
sodium on blood pressure in the free - living general population is limited . -
pubmed
Our results indicate that the
G - protein beta-3 subunit C825 T polymorphism is associated with higher
systolic blood pressure levels in a large free - living Japanese population , and more specifically in women with a low
sodium intake . -
pubmed
Relative quantification of
tau - related peptides using
guanidino - labeling derivatization ( GLaD ) with online - LC on a hybrid ion trap ( IT ) time - of - flight ( ToF ) mass spectrometer . -
pubmed
By in situ
dye labeling , we show that neonatal and adult SVZ progenitors differentially contribute to neurochemically distinct types of periglomerular interneurons in the
GL . -
pubmed
Deletions of the
sFRP-1 cysteine rich domain (
CRD ) lead to the complete loss of
Wnt antagonist function . -
pubmed
A region between
amino acids 73 - 86 within the second loop of the
CRD of
sFRP-1 was necessary for the optimal
Wnt inhibitory function . -
pubmed
Within this region , a conserved
tyrosine residue played a critical role , and its change to neutral or polar
amino acids lead to decreased
Wnt inhibitory activity . -
pubmed
The
sFRP-1 /
sFRP-2 chimera with the replacement of C - terminal 19
amino acids of
sFRP-1 with 11
amino acids of
sFRP-2 resulted in 70 % loss of activity indicating that
carboxyl - terminal region of
sFRP-1 is important for its Wnt inhibitory activity . -
pubmed
Samples were removed at predefined time points and analysed for
acetylsalicylic acid (
ASA ) and
salicylic acid (
SA ) content using stability - indicating HPLC . -
pubmed
LTD was mimicked and occluded by
mGluR agonists and blocked by nonselective
mGluR antagonist
( RS )- alpha - methyl-4 - sulfonophenylglycine (
MSPG ) but not by
N - methyl - D - aspartic acid ( NMDA ) receptor antagonist
2 - amino-5 - phosphonovaleric acid (
APV ) . -
pubmed
AIM : Radiation exposure to the kidney limits therapy with radiometal labelled
DOTATOC . -
pubmed
Hormones like ACTH ,
corticosteroids and
catecholamines as well as
cytokines ,
chemokines and alarmins play important roles in the initiation and persistence of the pro - inflammatory response after severe
injury . -
pubmed
Ca2 + entry through store - operated
Ca2 + channels drives the production of the pro - inflammatory molecule
leukotriene C4 (
LTC4 ) from mast cells through a pathway involving
Ca2 + - dependent protein kinase C ,
mitogen - activated protein kinases ERK1 / 2 ,
phospholipase A2 , and
5 - lipoxygenase . -
pubmed
Manipulating the amplitude and spatial extent of
Ca2 + entry by altering chemical and electrical gradients for
Ca2 + influx or changing the
Ca2 + buffering of the cytoplasm all impacted on
protein kinase C and
ERK activation , generation of
arachidonic acid and
LTC4 secretion , with little change in the bulk cytoplasmic
Ca2 + rise . -
pubmed
Similar bulk cytoplasmic
Ca2 + concentrations were achieved when CRAC channels were activated in 0.25 mm external
Ca2 + versus 2 mm
Ca2 + and 100 nm
La3 + , an inhibitor of
CRAC channels . -
pubmed
However , despite similar bulk cytoplasmic
Ca2 + ,
protein kinase C activation and
LTC4 secretion were larger in 2 mm
Ca2 + and
La3 + than in 0.25 mm
Ca2 + , consistent with the central involvement of a subplasmalemmal
Ca2 + rise . -
pubmed
Recombinant
TRPC3 channels also activated
protein kinase C , suggesting that subplasmalemmal
Ca2 + rather than a microdomain exclusive to CRAC channels is the trigger . -
pubmed
Enterostatin inhibition of angiogenesis : possible role of
pAMPK and
vascular endothelial growth factor A (
VEGF - A ) . -
pubmed
METHOD : To investigate this hypothesis , we studied the effect of
enterostatin as an
antiangiogenic agent in two angiogenic tissue culture model systems . -
pubmed
The
AMPK activator
5 - aminoimidazole-4 - carboximide ribonucleoside (
AICAR ) induced
VEGF - A expression . -
pubmed
The resemblance suggests that
L1CAM may be a target for
ethanol , and consistent with this idea ,
ethanol can inhibit
L1CAM adhesion in cell lines and
L1CAM - mediated outgrowth and signaling in cerebellar granule neurons . -
pubmed
However , it is not known whether
ethanol inhibits
L1CAM function in other neuron types known to require
L1CAM for appropriate development . -
pubmed
We find that
ethanol does not alter axonal polarization ,
L1CAM - dependent axon outgrowth or branching , or
L1CAM recycling in axonal growth cones . -
pubmed
Thus ,
ethanol inhibition of
L1CAM is highly dependent on neuronal context . -
pubmed
Free
copper at baseline correlated with the
ADL and IADL clinical scales scores at 1 year . -
pubmed
Site - specific mono - PEGylations were performed in different conformational regions of
Thymosin alpha 1 (
T alpha 1 ) by introducing one
cysteine residue into the chosen site and coupling with
thiol - specific
mPEG - MAL reagent . -
pubmed
Treatment of antisense
c - raf.1 oligomers enhanced
cytochrome P-450 content ( 81 % ) and reduced
glutathione S - transferase activity ( 33 % ) ,
UDP glucuronosyltransferase activity ( 74 % ) and
MDA concentration ( 30 % ) in carcinogen and antisense oligomer - treated group as compared with carcinogen control animals . -
pubmed
The SFs were grown on a commercially available
collagen mix that was placed onto the upper side of a Transwell
polycarbonate membrane . -
pubmed
BACKGROUND : Despite increasing evidence for the presence of voltage - gated
Na(+ ) channels (
Na ( v ) ) isoforms and measurements of
Na ( v ) channel currents with the patch -
clamp technique in arterial myocytes , no information is available to date as to whether or not
Na ( v ) channels play a functional role in arteries . -
pubmed
In parallel , using real time RT - PCR , we identified three
Na ( v ) transcripts :
Na(v)1.2 ,
Na(v)1.3 , and
Na(v)1.5 . -
pubmed
KB - R7943 , an inhibitor of the reverse mode of the
Na (+)/ Ca ( 2 + ) exchanger , mimicked the effect of
TTX and had no additive effect in presence of
TTX . -
pubmed
CONCLUSIONS / SIGNIFICANCE : These results define a new role for
Na ( v ) channels in arterial physiology , and suggest that the
TTX - sensitive
Na ( v ) 1.2 isoform , together with the
Na (+)/ Ca ( 2 + ) exchanger , contributes to the contractile response of aortic myocytes at physiological range of membrane depolarization . -
pubmed
The distribution of
PAHs and
PCDD / Fs in different MSWI unit ashes , including bottom residue (
BR ) , fly ash pit (
FAP ) , fabric filter ( FF ) , semi - dryer absorber ( SDA ) , economizer ( EC ) and super heater ( SH ) , has seldom been discussed . -
pubmed
Average
PAHs content in ash samples from
BR ,
FAP , FF , SDA , EC and SH were found to be 0.020 , 0.237 , 0.234 , 0.125 , 0.037 and 0.034 microg TEQ g(-1 ) , respectively . -
pubmed
Furthermore ,
PAHs in ashes were mainly found in
BR ( 41 % ) and
FAP ( 31 % ) . -
pubmed
The
FAP ash included 18 % of
PAHs coming from FF ash . -
pubmed
On the other hand ,
PCDD / Fs content in
BR ,
FAP , FF ,
SDA , EC and SH was found to be 0.0329 , 1.1589 , 1.2807 , 0.1476 , 0.6868 and 0.0921 ng I - TEQ g(-1 ) , respectively . -
pubmed
The
FAP ash included 33 % of
PCDD / Fs coming from FF ash . -
pubmed
Therefore ,
PCDD / Fs content in
FAP and FF exceeded the legal limit of 1 ng I - TEQ g ( -1 ) ; therefore , a solidification process is necessary before landfilling in Taiwan . -
pubmed
Treatment usually consists of
carvedilol and an
angiotensin - converting enzyme inhibitor or
angiotensin II receptor blocking agent if left ventricular ejection fraction is less than 40 % . -
pubmed
Next , Experimental test
10H2DA how to inhibited the activities of
MMPs : with RASFs isolated from rheumatoid tissues by enzymatic digestion , cultures in monolayers were treated with
10H2DA ( 0.5mM , 1mM , and 2mM ) or
PBS for 2h followed by stimulation with
TNF - alpha ( 10 ng / ml ) for 2h , mRNA . -
pubmed
RESULTS : The molecular investigation revealed that the
10H2DA - mediated suppression was likely to occur through blocking
p38 kinase and
c - Jun N - terminal kinase -
AP-1 signaling pathways . -
pubmed
In contrast ,
10H2DA had no effect on
extracellular signal - regulated kinase activity ,
NF - kappaB DNA - binding activity and
IkappaBalpha degradation . -
pubmed
The anti -
CD20 monoclonal antibody
rituximab is an effective treatment for
small lymphocytic lymphoma ; however , it has been associated with infusion reactions , including
cardiac arrhythmias . -
pubmed
Amino acid starvation by
asparaginase (
ASNase ) enhances phosphorylation of
eukaryotic initiation factor 2 (
eIF2 ) by
general control nonderepressible 2 (
GCN2 ) kinase , leading to reduced global mRNA translation rates . -
pubmed
This study addressed the importance of
GCN2 for the immune system to adapt to
amino acid starvation by
ASNase . -
pubmed
These results indicate that loss of
GCN2 enhances immunosuppression by
ASNase and that this
eIF2 kinase is broadly required for
amino acid stress management in the immune system . -
pubmed
Dimerization and its role in
GMP formation by
human guanylate binding proteins . -
pubmed
Human guanylate binding proteins (
hGBP-1 and 2 ) belonging to
large GTPases have the unique feature of hydrolyzing
GTP to a mixture of
GDP and
GMP with unequal ratios . -
pubmed
Remarkably , the
enzyme exhibits differential
GTPase activities depending on dimerization ; a monomer produces only
GDP , but a dimer gives both
GDP and
GMP with stimulation of the activity . -
pubmed
Similar to
hGBP-1 ,
hGBP-2 showed dimerization - related
GTPase activity for
GMP formation , indicating that this family of proteins follows a broadly similar mechanism for
GTP hydrolysis . -
pubmed
The obtained
copper ( I ) complexes were characterized in detail by time - dependent 1D [
( 1 ) H ,
( 13 ) C ] and 2D [
( 1 ) H - NOESY ] NMR spectroscopy , elemental analysis , high - resolution ESI -
TOF mass spectrometry , and analytical ultracentrifugation . -
pubmed
CONCLUSION : These findings indicate that alterations in high -
mannose type N - glycans and N - glycogenes in chondrocytes correlate with the release of
MMP-13 and
ADAMTS-5 during cartilage degradation . -
pubmed
dexamethasone , preoperative administration of analgesia including the use of non - steroidal anti - inflammatory or
COX II inhibitors , intraoperative use of an anti - emetic , pre - incisional use of
bupivacaine , administration of intraperitoneal
bupivacaine on establishment of
pneumoperitoneum , and avoidance of drains . -
pubmed
For the
OPA1 gene , the exons were amplified by PCR and mutations were detected by restriction enzymes or the
dye terminator method . -
pubmed
Displacement was defined as the length between the center of gravity of
titanium markers and the needle applicator tips along the daily
CT axis . -
pubmed
Inflow and outflow samples were collected and analysed for Total Suspended Solids ( TSS ) , Total
Phosphorus (
TP ) and Total
Nitrogen (
TN ) . -
pubmed
Histone deacetylase-2 (
HDAC-2 ) plays a pivotal role in many cases of
steroid insensitivity . -
pubmed
The main aim of this study was to restore the smoking - induced reduction in
corticosteroid sensitivity by increasing
HDAC-2 activity using low - dose
theophylline . -
pubmed
These results indicate that low - dose
theophylline might provide protection from
smoke damage and improve the anti - inflammatory effects of
steroids by increasing
HDAC-2 activity . -
pubmed
A conserved N - terminal
arginine - motif in
GOLPH3 - family proteins mediates binding to coatomer . -
pubmed
We identify a cluster of
arginine residues at the N - terminal end of
GOLPH3 proteins that are necessary and sufficient to mediate coatomer binding . -
pubmed
We establish that the oligomerization status and
phosphatidylinositol-4 - phosphate - binding properties of
Vps74p largely account for the membrane - binding capacity of the protein and identify an
Arf1p -
Vps74p interaction as a potential contributing factor in
Vps74p Golgi membrane association . -
pubmed
To ascertain whether the defect in
tdy2 leaves affected the movement of
sucrose into the phloem or its long - distance transport , we performed radiolabeled and fluorescent dye tracer assays . -
pubmed
The disruption of
sucrose movement in the phloem in
tdy2 mutants provides evidence that the
Tdy2 callose synthase functions in vascular maturation and that the
vascular defects result in impaired symplastic trafficking into the phloem translocation stream . -
pubmed
Clinical and preclinical data suggest that
ZOL may have also anti - angiogenic activity ; however , the effects of Dmab ( a fully humanized antibody against the
receptor activator of nuclear factor kappa B ligand ) on angiogenesis are largely unknown . -
pubmed
FDG - PET and PET /
CT are useful methods to identify malignant change in
neurogenic tumors in
NF1 and to discriminate malignant from benign
neurogenic lesions . -
pubmed
Administration of
trona significantly reduced ( p & lt ; 0.05 )
alkaline phosphatase activity in the liver and kidney with corresponding increases in the serum enzyme . -
pubmed
Blood samples at 0 , 20 , 40 , 60 min of the standard mixed meal were collected to test plasma
glucose ,
serum insulin and
C peptide . -
pubmed
The area under the curve ( AUC ) of plasma
glucose and
serum C peptide was significantly lower after the INJEX30 injection than that after
insulin pen injection [ plasma
glucose AUC ( 542 + - 172)min mmol L(-1 ) vs ( 601 + - 199 ) min mmol L(-1 ) , P & lt ; 0.01 ; C peptide AUC ( 70 + - 53 ) min microg L(-1 ) vs ( 80 + - 58 ) min microg L(-1 ) , P & lt ; 0.01 ] . -
pubmed
Phenome - wide association studies on a quantitative trait : application to
TPMT enzyme activity and
thiopurine therapy in pharmacogenomics . -
pubmed
We tested our approach on
thiopurine S - methyltransferase (
TPMT ) activity in patients treated by
thiopurine drugs . -
pubmed
Data after initiation of
thiopurine treatment were analyzed and patient groups were compared according to their
TPMT activity level . -
pubmed
In pretreated
NSCLC ,
EGFR TKIs are considered more effective than standard monotherapy with cytotoxics in presence of classical
EGFR mutations , whereas in the
EGFR wild - type population , a similar efficacy to
docetaxel or
pemetrexed in term of survival has been demonstrated . -
pubmed
Enteral feeds contributed
16.2 % to overall administered
sodium , and were the major source in patients in the PICU for & gt ; 10 days . -
pubmed
The mean fasting plasma
glucose level at onset of
HNF1beta -
MODY was considerably higher and the age of onset of
HNF1beta -
MODY was considerably older than they were for
HNF1alpha -
MODY , while the mean
BMI and
C - peptide index at onset were similar . -
pubmed
A significant improvement in anti - Toxoplasma effect among
quinoline derivatives was detected in B11 ,
B12 ,
B23 , and B24 , whose structures carry two
quinoline rings , and their resultant cLogP values are & gt;=7 . -
pubmed
When treated with
quinoline ,
8 - hydroxyquinoline or
B23 , 40 - 45 % of the parasites lost their apicoplasts . -
pubmed
More importantly ,
B12 and
B23 which harbor superior anti -
cancer properties than
chloroquine , have effective anti - Toxoplasma activity . -
pubmed
Per protocol (
PP ) analysis , including only participants who accessed the interventions and also reported accessing additional outside interventions during the trial , showed that about 75 % of extended self - help participants moved from probable dependence , harmful or hazardous use to lower
alcohol use levels at 3- and 6- and 12 - month follow - ups , compared to about 40 - 60 % of brief intervention users and controls who accessed extra help ( p & lt ; 0.05 ) . -
pubmed
In contrast ,
PP analysis suggested that cognitive - behavioral extended self - help in combination with other interventions was more effective in changing
alcohol use than brief intervention or assessment only . -
pubmed
The substrate - binding cleft in
SpPth is formed with three loops : the gate loop ,
Ile91 -
Leu102 ; the base loop ,
Gly108 -
Gly115 ; and the lid loop ,
Gly136 -
Gly150 . -
pubmed
Effects of
melatonin on prenatal
dexamethasone - induced epigenetic alterations in hippocampal morphology and
reelin and
glutamic acid decarboxylase 67 levels . -
pubmed
The programming effects of prenatal
dexamethasone exposure on the brain were assessed at postnatal days (
PND ) 7 , 42 , and ~120 . -
pubmed
At ~PND120 ,
melatonin restored the reduced levels of hippocampal
reln and
GAD1 mRNA expression in the DEXM group . -
pubmed
In addition ,
melatonin restored the
reln mRNA expression levels by ( 1 ) reducing
DNA methyltransferase 1 (
DNMT1 ) mRNA expression and ( 2 ) reducing the binding of
DNMT1 and the
methyl - CpG binding protein 2 (
MeCP2 ) to the
reln promoter . -
pubmed
The present study showed that prenatal
dexamethasone exposure induced gross alterations in hippocampal morphology and reduced the levels of hippocampal mRNA expression of
reln and
GAD1 . -
pubmed
Thus ,
melatonin had a beneficial effect in restoring hippocampal
reln mRNA expression by reducing
DNMT1 and
MeCP2 binding to the
reln promoter . -
pubmed
Previous studies in
vanadium toxicity showed involvement of oxidative stress in isolated liver hepatocytes and mitochondria via increasing of ROS formation , release of
cytochrome c and
ATP depletion after incubation with different concentrations ( 25 - 200 microM ) . -
pubmed
Aromatic L - amino acid decarboxylase cells in the spinal cord : a potential origin of
monoamines . -
pubmed
This compound goes on to display a
coil - globule transition above a certain threshold of
acetone . -
pubmed
Rapamycin is helpful in the treatment of certain
cancers by inhibiting
mTOR (
mammalian target of rapamycin ) pathway . -
pubmed
Western blot assay showed that
rapamycin led to release of
cytochrome c from mitochondrial membranes to cytosol . -
pubmed
Further Western blot assays showed that
rapamycin induced activation of
caspase-9 and
caspase-8 and the cleavage of
caspase-3 . -
pubmed
Rapamycin induced cleavages of
caspase-3 and apoptosis was inhibited by both
Z - LETD - FMK and
Z - IETD - FMK treatment . -
pubmed
Postharvest treatment of ' Gala ' with
propylene did not affect the internal
ethylene concentration of the fruit but 1 -
MCP markedly inhibited it . -
pubmed
Bioinformatic , phenotypic , and gene expression analyses demonstrated that the
KdgR regulon included genes involved in uptake and metabolism of molecules resulting from pectin degradation as well as those central to the utilization of a number of other
carbon sources . -
pubmed
Trichloroacetic acid (
TCA ) was used to precipitate the
protein and separate the dextran sulfate . -
pubmed
Detection and quantitation of dextran sulfate was achieved by
post column reaction with
dimethylene blue to form a metachromatic complex that absorbs visible light at 530 nm . -
pubmed
The
enzyme was strongly inactivated by
serine protease inhibitor
PMSF . -
pubmed
The values of Km and Vmax for racemic
2,2 - dimethylcyclopropane carboxamide (
DMCPCA ) were 4.58 mM and 35.03 mumol min(-1 ) mg(-1 )
protein , respectively . -
pubmed
The
amidase showed a broad substrate spectrum toward
aliphatic , aromatic and heterocyclic amides , but could hardly hydrolyze the bulky side - chain - containing
amides . -
pubmed
Furthermore , kinetic resolution of
racemic DMCPCA by the
amidase afforded
S - DMCPCA in 46.3 % yield and 99 % ee with an average E - value of 67 . -
pubmed
These unique properties of the
amidase imply that it is a promising biocatalyst for the production of chiral
amides and
carboxylic acids . -
pubmed
A cocktail approach , with dosages of the probe drugs
caffeine ( 3.0 mg / kg ) ,
metoprolol ( 2.33 mg / kg ) and
midazolam ( 0.45 mg / kg ) , was used to measure
cytochrome P450 (
CYP ) metabolic activities . -
pubmed
Tet had no or minor impact on the pharmacokinetics and metabolism of the probe drugs
caffeine and
metoprolol ,
CYP1A2 and
CYP2D6 substrates , respectively . -
pubmed
However ,
Tet increased AUC0 - 24 h and decreased AUCratio(0 - 24 h ) (
1 - hydroxymidazolam /
midazolam ratio ) for
midazolam statistically significant , both in single or multiple dosing of
Tet , with up to 39 or 57 % increase for AUC0 - 24 h and 29 % or 22 decrease for AUCratio(0 - 24 h ) , respectively , in line with previous in vitro findings for its
CYP3A4 inhibition . -
pubmed
The extensive use of
Tet and herbal medicines containing
Tet makes
Tet a candidate for further evaluation of
CYP3A - mediated herb - drug interactions . -
pubmed
Although
VMAT2 is known to package
monoamines into synaptic vesicles , in VENs and fork cells its expression occurs in the absence of
monoamine - synthesizing enzymes or reuptake transporters . -
pubmed
Compared to low
LDL cholesterol , high
LDL cholesterol was associated with longer RFS in univariable analysis ( continuous : Hazard Ratio ( HR ): 0.67 , 95%-Confidence Interval ( CI ): 0.47 - 0.96 , P = 0.03 ; 3 mM cut - point : HR : 0.44 , 95%-CI : 0.24 - 0.79 , P = 0.006 ) . -
pubmed
Preoperative
LDL cholesterol remained an independent predictor for
PCa recurrence in a multivariable model adjusted for age , preoperative
PSA , statin use ,
tumor stage , Gleason score ,
nodal status and
surgical margin status ( continuous : HR : 0.66 , 95%-CI : 0.44 - 0.99 , P = 0.04 ; 3 mM cut - point : HR : 0.41 , 95%-CI : 0.21 - 0.78 , P = 0.007 ) . -
pubmed
Acetate reduction to
acetaldehyde and further to
ethanol via an
aldehyde : ferredoxin oxidoreductase (
AOR ) and
alcohol dehydrogenase has been postulated alongside the classic pathway of
ethanol formation via a
bi - functional aldehyde / alcohol dehydrogenase (
AdhE ) . -
pubmed
Here we demonstrate that
AOR is critical to
ethanol formation in acetogens and inactivation of
AdhE led to consistently enhanced autotrophic
ethanol production ( up to 180 % ) . -
pubmed
The
aor1 + 2 double knockout
strain lost the ability to convert exogenous
acetate ,
propionate and
butyrate into the corresponding
alcohols , further highlighting the role of these enzymes in catalyzing the thermodynamically unfavourable reduction of
carboxylic acids into
alcohols . -
pubmed
Several mechanisms have been proposed including altered
glucose and
fatty acid metabolism , hepatic
fatty acid storage , and modulation of
glucagon - like peptide ( GLP )- 1 . -
pubmed
A rapid and selective method for the quantitation of neurotransmitters ,
l - Glutamic acid (
GA ) and
gamma - Aminobutyric acid (
GABA ) , was developed and validated using gas chromatography - tandem mass spectrometry (
GC -
MS /
MS ) . -
pubmed
The method calibration was linear from 0.5 to 100mug / mL , with limits of detections of 100ng / mL and 250ng / mL for
GA and
GABA , respectively . -
pubmed
Furthermore , these results suggest that loss of
Oat1 or
Oat3 function in isolation does not result in significant alterations in brain tissue levels of
GA or
GABA . -
pubmed
Expert commentary : In this report , we review the up - to - date findings of more potency roles of
silibinin in
beta - thalassemia ( beta -
TM ) ,
acute myeloid leukemia (
AML ) ,
anaplastic large cell lymphoma (
ALCL ) and
multiple myelomas (
MM ) therapy and attempt to clarify the mechanisms underlying its effects . -
pubmed
Courses were primarily dispensed to men who have sex with men ( 84 % ) and consisted overwhelmingly of a three - drug antiretroviral therapy regimen containing two
nucleoside reverse transcriptase inhibitors and either an
integrase inhibitor (
raltegravir ) or a boosted
protease inhibitor (
lopinavir /
ritonavir ) . -
pubmed
The deoxyribonucleic acid ( DNA ) repair gene encoding the
excision - repair cross - complementation group 1 (
ERCC1 ) protein is known to predict the response to
platinum - based chemotherapy . -
pubmed
Hepatic
superoxide dismutase (
SOD ) and
malondialdehyde (
MDA ) were detected . -
pubmed
Our results showed marked increase in all measured parameters except
SOD , TAC ,
eNOS immunoexpression and Bcl2 mRNA gene expression which decreased in
Cd induced
hepatotoxicity group . -
pubmed
NEB showed marvelous protective effect against
Cd induced changes . -
pubmed
NEB decreased liver enzymes (
ALT and
AST ) , mean arterial pressure , total
cholesterol levels ,
MDA ,
iNOS immunoexpression and TNF - alpha gene expression but significantly increased
SOD , TAC ,
eNOS immunoexpression and
Bcl-2 gene expression . -
pubmed
Moreover ;
NEB markedly improved the histopathological changes induced by
Cd . -
pubmed
These findings prove the antioxidant , anti - apoptotic and anti - inflammatory properties of
NEB and its protective role in
Cd induced
hepatotoxicity . -
pubmed
Lipid bilayer - coated mesoporous
silica nanoparticles carrying
bovine hemoglobin towards an erythrocyte mimic . -
pubmed
Hemoglobin (
Hb)-loaded mesoporous
silica nanoparticles ( MSNs ) coated with a lipid bilayer ( LB - MSNs ) were investigated as an erythrocyte mimic . -
pubmed
Furthermore the encapsulated
Hb exhibits similar
peroxidase - like activity in catalyzing the oxidation of
2,2 '- azino - bis ( 3 - ethylbenzothiazoline-6 - sulfonic acid ) diammonium salt (
ABTS ) with
hydrogen peroxide . -
pubmed
The natural course of classical
diabetic nephropathy is initially
microalbuminuria or moderately increased urine
albumin excretion ( 30 - 300mg / g
creatinine ) . -
pubmed
Multifactorial intervention targeting
glucose , lipids and blood pressure including blockade of
renin angiotensin system and lifestyle , has improved renal and cardiovascular prognosis and reduced mortality with 50 % . -
pubmed
Carboplatin in
BRCA1 / 2 - mutated and triple - negative
breast cancer BRCAness subgroups : the
TNT Trial . -
pubmed
HR also repairs DNA
lesions caused by
platinum agents and
PARP inhibitors . -
pubmed
Triple - negative
breast cancers (
TNBCs ) harbor subpopulations with
BRCA1 / 2 mutations , hypothesized to be especially
platinum - sensitive . -
pubmed
Cancers in putative ' BRCAness ' subgroups -
tumors with
BRCA1 methylation ; low levels of
BRCA1 mRNA (
BRCA1 mRNA - low ) ; or mutational signatures for
HR deficiency and those with basal phenotypes - may also be sensitive to
platinum . -
pubmed
We conclude that patients with advanced
TNBC benefit from characterization of
BRCA1 / 2 mutations , but not
BRCA1 methylation or Myriad
HRD analyses , to inform choices on
platinum - based chemotherapy . -
pubmed
Hypotension , but not the increase in colorectal pressure , was inhibited by a 10 - min pretreatment with the
NK1 receptor antagonist
CP-99,994 . -
pubmed
In a pilot experiment using subcutaneous ( SC ) injection , a similar dose range of
LMN - NKA ( 3 - 100 mug / kg ) again appeared to increase colorectal pressure with a similar AUC ( up to 18,546 mmHg*s ) to that seen after
IV injection , but lower peak amplitude ( up to 49 mmHg ) . -
pubmed
Results : The differences between the groups ' levels of HbA1c ( P=0.02 ) and
HDL ( P=0.02 ) were significant , but the differences between their
FBS ,
cholesterol ,
triglycerides and
LDL were not significant ( P>0.05 ) . -
pubmed
The reproduction assays gave EC50 and EC10 values , respectively , of
6.3 and 1.4 mg a.s./kg dry soil for
abamectin ; 1.0 and 0.12 mg a.s./kg dry soil for Kraft 36 EC ; and 54 and 23 mg a.s./kg dry soil for Score 250 EC . -
pubmed
PURPOSE : To compare and evaluate bone regeneration with and without combining platelet rich plasma (
PRP ) ,
calcium sulfate (
CS ) and autogenous bone graft in
periapical defects of jaw . -
pubmed
In the first group ( study group ) the bone defect was filled with
PRP ,
calcium sulfate and autologous bone graft . -
pubmed
In the second group ( control group ) defect was allowed to heal without
PRP ,
calcium sulfate and autogenous bone graft . -
pubmed
Thus the grouping of
PRP , combined
calcium sulfate with autologous bone graft proved to be a novel osteoinductive biomaterial . -
pubmed
TGR was significantly reduced by 12 weeks with
lanreotide versus placebo ( difference in least - square mean TGR0 - 12 of - 2.9 [ - 5.1 , - 0.8 ] , p = 0.008 ) , a difference that was maintained at most subsequent visits . -
pubmed
baumannii cultures from cerebrospinal fluid (
CSF ) showed extensive resistance to the tested antibiotics except for
tigecycline and
colistin . -
pubmed
While
glimepiride stimulates beta - cell secretion and leads to a reduction of blood
glucose levels ,
pioglitazone activates
peroxisome proliferator - activated receptor - gamma and improves
insulin resistance . -
pubmed
The Combined Use of
Phenothiazines and Statins Strongly Affects
Doxorubicin - Resistance , Apoptosis , and
Cox-2 Activity in
Colon Cancer Cells . -
pubmed
Additionally ,
COX-2 activity was diminished , and
caspase-3 activity was increased to a higher extent by
phenothiazine derivative :
simvastatin mixtures than by
phenothiazine derivatives themselves . -
pubmed
We found that
SP2509 , a selective inhibitor of
LSD1 , specifically promoted the differentiation of
Utx - null
HSPCs while sparing wild - type
HSPCs . -
pubmed
In contrast ,
SP2509 treatment reversed these changes in gene expression in
Utx - null
HSPCs . -
pubmed
Accordingly ,
Utx loss decreased H3K4 methylation level probably through the COMPASS - like complex , while
LSD1 inhibition by
SP2509 partially reversed the reduction of H3K4 methylation in
Utx - deficient
HSPCs . -
pubmed
In contrast ,
DbLcyE preferred to convert
lycopene into
monocyclic delta - carotene , and possessed a weak
beta - monocyclase activity . -
pubmed
However , it is rarely reported whether indirect exposure to
TCDD can cause similar negative impact on
F3 . -
pubmed
Results showed that in the
F3 generation 500 ng / kg BW / day
TCDD group , ovarian coefficient ,
LH concentration in serum and number of primary follicles were decreased , and the apoptosis of granular cells was significantly increased . -
pubmed
Our data showed that ancestral
TCDD exposure may impair transgenerational adult ovary development and functions , which may be related to an inhibition of the
Igf2 /
H19 pathway in the ovarian . -
pubmed
The developed approach has been tested using
HDAC4 siRNA as a model therapeutics , while the application can also be extended to other gene therapeutics including micro RNA ( miRNA ) , plasmids
oligonucleotides , etc . -
pubmed
The
amino acid sequence of
S proein and its interaction with the
ACE-2 protein is mimicked with the aid of Hill function . -
pubmed
Although the mechanisms of action underlying the
glucose - lowering properties of
HCQ are still not entirely clear , evidence suggests that this drug may exert multifaceted effects on
glucose regulation , including improvement of
insulin sensitivity , increase of
insulin secretion , reduction of hepatic
insulin clearance , and reduction of
systemic inflammation . -
pubmed
Letter to the editor : " Comment on : Application of the optimized
carbon monoxide rebreathing method for the measurement of total
haemoglobin mass in
chronic liver disease " . -
pubmed
This shark displays hormonally controlled bioluminescence in which
melatonin ( MT ) and
prolactin (
PRL ) trigger light emission , while
alpha - melanocyte - stimulating hormone (
alpha - MSH ) and
adrenocorticotropic hormone (
ACTH ) play an inhibitory role . -
pubmed
Our results reveal the implication of Es -
Opn3 ,
MT ,
inositol triphosphate (
IP3 ) , intracellular
calcium ,
calcium - dependent
calmodulin and
dynein in the ILS cell pigment aggregation . -
pubmed
The structure , morphology , and valence states of surface elements of as - prepared catalysts were characterized by XRD ,
TEM , SEM ,
N2 adsorption - desorption , XPS , and
H2 - TPR . -
pubmed
BACKGROUND : Lorvotuzumab mertansine (
IMGN901 ) is an antibody - drug conjugate linking an antimitotic agent (
DM1 ) to an anti -
CD56 antibody ( lorvotuzumab ) . -
pubmed
Only one study showed a positive effect after supplementation with 60,000 IU / month of
vitamin D3 for 12 months , with a significant reduction in the concentrations of
glycated hemoglobin , fasting
glucose , and two - hour postprandial
glucose . -
pubmed
Further , the molecular interactions between
erythrin and the
diabetes related target proteins was established by analysing the interactions with associated
amino acids . -
pubmed
We aimed to evaluate the effect of the inhibitors of
proprotein convertase subtilisin / kexin type 9 (
PCSK9 - i ) on
steatosis biomarkers such as
triglyceride -
glucose index ( TyG ) and
hepatic steatosis index ( HSI ) and analyse the role of
TG / HDL in this population before and after adding - on
PCSK9 - i. -
pubmed
Basolateral Amygdala Serotonin 2C Receptor Regulates
Emotional Disorder - Related Symptoms Induced by Chronic
Methamphetamine Administration . -
pubmed
Molecular and morphological evidence showed that chronic
MA administration reduced the expression of the
5 - hydroxytryptamine ( 5 - HT ) rate - limiting enzyme ,
tryptophan hydroxylase 2 , in the dorsal raphe and the concentrations of
5 - HT and its metabolite
5 - hydroxyindoleacetic acid in the basolateral amygdala ( BLA ) nuclei . -
pubmed
Neuropharmacology and virus - mediated silencing strategies confirmed that targeting
5 - HT2CR reversed the
depressive and anxious behaviors induced by chronic
MA administration . -
pubmed
Thus , herein , for the first time , we report that the abnormal regulation of
5 - HT2CR is involved in the manifestation of
emotional disorder - like symptoms induced by chronic
MA use . -
pubmed
Our study suggests that
5 - HT2CR in the BLA is a promising clinical target for the treatment of
MA - induced
emotional disorders . -
pubmed
Endogenous
SO2 - dependent
Smad3 redox modification controls
vascular remodeling . -
pubmed
Notably , ~42 % of these sulfenylated
cysteines are dynamically regulated by
SO2 , among which is
cysteine-64 of
Smad3 ( Mothers against
decapentaplegic homolog 3 ) , a key transcriptional modulator of
transforming growth factor beta signaling . -
pubmed
Sulfenylation of
Smad3 at
cysteine-64 inhibits its DNA binding activity , while mutation of this site attenuates the protective effects of
SO2 on
angiotensin II - induced
vascular remodeling and
hypertension . -
pubmed
Investigations on new
tripeptidyl - p - nitroanilide substrates for
subtilisins . -
pubmed
Administration of 10(-9)-10(-7 ) mol / L
ET caused dose - dependently severe
contracture of myocytes , increase in cytosolic
lactate dehydrogenase leakage and increase of cellular total
calcium . -
pubmed
ET also enhanced
45Ca2 + influx in myocytes , that could be antagonized by
calcium channel blocker
verapamil . -
pubmed
Incubation of
Fura-2 -preloaded myocytes with
ET would induce a significant increase of cytosolic free
Ca2 + , and this effect of
ET could also be partly antagonized by
verapamil . -
pubmed
These results suggest that
ET enhances
Ca2 + influx in myocytes mainly through
voltage - dependent calcium channel , by which
ET exerts its biological action . -
pubmed
The results suggest that
Mg2 + ions promote binding by an action at the
opioid receptor , even in the absence of
G protein , and that opioid antagonists may differ in their recognition of opioid receptor binding sites . -
pubmed
[ The effect of
elastase on
aminonucleoside nephrosis -- on renal vascular system ] . -
pubmed
, or
PAN+
elastase - treated ( 5 mg / kg / day i. m. ) group . -
pubmed
These changes , which progressed over a period of 40 min after
indomethacin was injected , probably result from renal
oligemia and decreased urine production , with an associated decrease in
T2 - values . -
pubmed
We have explored the effect of the
protein kinase inhibitor H7 on tight junction formation in a MDCK cell model for the development of cell - cell contact , tight junctions and epithelial polarity : the "
Ca ++ switch " model . -
pubmed
In this developmental model , which is thought to mimic processes during the early morphogenesis of epithelial tissues , the
protein kinase inhibitor H7 markedly inhibits the development of transepithelial resistance of confluent MDCK cells during the " switch " from low ( 1 - 5 microM ) to normal ( 1.8 mM )
Ca++ media compared with control MDCK cells . -
pubmed
Moreover , indirect immunofluorescence using specific antisera against two tight junctional proteins ,
ZO1 and
cingulin , revealed that
H7 inhibits the sorting of these proteins from an intracellular site to the lateral surfaces of MDCK cells when the
Ca ++ in the medium is raised . -
pubmed
Trimming inhibitors , a second generation of inhibitors of glycosylation , interfere with the processing of
oligosaccharides by specific
glucosidases and
mannosidases , resulting in a block of the conversion of high -
mannose to complex - type
oligosaccharides . -
pubmed
Immunoprecipitation using the
monoclonal antibodies GR - J2C / 6 and
GR - E6B / 3 followed by
SDS - PAGE analysis has led to the following results :
GR - J2C / 6 precipitated two molecules with apparent molecular weights of 58 and 60 kD ; the antigen recognized by
GR - E6B / 3 was a molecule with molecular weight of 68 kD. -
pubmed
In includes three successive chromatographies of total tRNA on
C18 - modified Lichrosphere Si-1000 in
acetonitrile gradients . -
pubmed
Clinically ,
leukopenia with
neutropenia and low plasma levels of
copper and
ceruloplasmin were seen in all cases . -
pubmed
ACTH and
cortisol production decreases , metabolism slows down and due to an
adrenal insufficiency for which the pituitary is responsible there are characteristic changes in the
cortisol - dependent metabolic processes . -
pubmed
The formation of electronically excited states during
hydroperoxide metabolism is analysed in terms of recombination reactions involving secondary
peroxyl radicals and scission of the
O - O bond of
peroxides by haemoproteins , mainly
myoglobin . -
pubmed
Endogenic phosphorylation of
acetyl - CoA - carboxylase completely correlates with its inactivation and depends on the
citrate level . -
pubmed
About 68 % of individuals receiving
PEP had exposures to
bites , especially by dogs , and nearly 30 % of
PEP cases were due to non -
bite exposures . -
pubmed
In contrast , with
phosvitin as the exogenous substrate , higher doses of
polyamines , especially
spermine , inhibited phosphorylation . -
pubmed
Cytosol
protein kinase activity eluted from
DEAE - cellulose at 0.2 - 0.3 M
NaCl . -
pubmed
[ Effect of " heavy "
phenamines on
alpha - and beta - adrenoreceptors ] . -
pubmed
The subunit composition of
human myeloperoxidase was studied with the use of
sodium dodecyl sulphate /
polyacrylamide - gel electrophoresis and gel filtration . -
pubmed
The 57 000 - Mr and 39 000 - Mr forms had very similar
amino acid composition and yielded an apparently identical collection of fragments on incubation with
CNBr . -
pubmed
Two hypotheses have been investigated : either a rapid action of
glucosidases on the liberated
phosphooligosaccharides or a preferential splitting of the non - glucosylated population of
dolichyl diphosphate oligosaccharides . -
pubmed
Addition of
p - nitrophenyl - alpha - D - glucoside inhibits
glucosidase activities and allows the production of a major population of
dolichyl diphosphate oligosaccharides containing three
glucose residues . -
pubmed
thiosulphate and
cyanide , provides additional evidence of
Ping - Pong Bi - Bi mechanism for this
transferase . -
pubmed
NMR studies of
6 - fluoropyridoxal 5 '- phosphate - containing cytosolic
aspartate aminotransferase . -
pubmed
Sucrose also led to an early rise in the activity of hepatic
triokinase , but did not influence
ketohexokinase or
fructose-1 - phosphate aldolase . -
pubmed
The comparison between the pre- and
post - dialysis incorporation values ( 3900 + /- 660 cpm vs 9580 + /- 1600 cpm , n = 8) showed a significant increase of the
3H - Thymidine incorporation in the presence of
post - dialysis plasma ( paired t test : p less than 0.01 ) which did not differ from that of control plasma . -
pubmed
Serum
PSA and
testosterone levels were measured monthly . -
pubmed
The positive margin rate was not increased after reevaluation with
cytokeratin ,
PSA and
prostatic acid phosphatase immuno - staining but of 4 cases initially staged as
P0 on
hematoxylin and eosin evaluation 2 had microscopic foci of
cancer with
prostatic acid phosphatase staining . -
pubmed
Immuno - staining with the proliferation markers
proliferation cell nuclear antigen and
Ki-67 showed decreased staining in surgical specimens relative to pretreatment needle biopsy specimens , which suggests that outgrowth of
androgen independent clones does not develop during prolonged neoadjuvant therapy . -
pubmed
CONCLUSIONS : Eight months of neoadjuvant
androgen withdrawal therapy results in low positive margin rates and
PSA nadir levels . -
pubmed
The initial rapid decrease in
PSA results from cessation of
androgen regulated
PSA synthesis and apoptosis , while the ongoing slower decrease reflects decreasing
tumor volume . -
pubmed
Polymerization of
fibrin monomers in the presence and absence of
calcium ions was strongly impaired . -
pubmed
SDS - polyacrylamide gel electrophoresis of reduced
fibrinogen showed normal
B beta - and gamma - chains , whereas no normal
A alpha - chain was detected in the proposita . -
pubmed
Immunoblot analysis with the
monoclonal antibody Y18 , detecting an epitope within the stretch of
amino acids A alpha 1 - 51 , revealed an
A alpha - chain of about 50 kDa with an intact amino terminus . -
pubmed
Immunoblotting with antibodies directed against
serum albumin demonstrated the presence of
albumin covalently linked to
fibrinogen via a
disulfide bridge . -
pubmed
Radicicol , a
protein tyrosine kinase inhibitor , suppresses the expression of
mitogen - inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide and in experimental
glomerulonephritis . -
pubmed
Thus ,
COX-2 instead of
COX-1 is implicated to produce
prostanoids mediating inflammatory responses . -
pubmed
Radicicol inhibited
tyrosine phosphorylation of
p53 / 56lyn , a
Src family tyrosine kinase and one of the major
tyrosine - phosphorylated proteins in LPS - stimulated macrophages . -
pubmed
The enzyme activity of
COX-1 or
COX-2 was not affected by
radicicol in macrophages . -
pubmed
Other
protein tyrosine kinase inhibitors suppressed the LPS - induced
COX-2 expression in macrophages but at much higher concentrations than needed for
radicicol . -
pubmed
Radicicol did not inhibit the
COX-2 expression induced by
phorbol 12 - myristate 13 - acetate in macrophages . -
pubmed
The magnitude of the inhibition of
COX-2 protein synthesis by
radicicol was much greater than that of the steady state levels of
COX-2 mRNA . -
pubmed
These results suggest that
radicicol inhibits
COX-2 expression mainly at
post - transcriptional steps . -
pubmed
Role of the
Cys 2 -
Cys 10
disulfide bond for the structure , stability , and folding kinetics of
ribonuclease T1 . -
pubmed
The
Cys 2 -
Cys 10
disulfide bond in
ribonuclease T1 was broken by substituting
Cys 2 and
Cys 10 by
Ser and
Asn , respectively , as present in
ribonuclease F1 . -
pubmed
The thermodynamic stability of
ribonuclease T1 is strongly reduced by breaking the
Cys 2 -
Cys 10 bond , and the free energy of denaturation is decreased by about 10 kJ / mol . -
pubmed
Apparently , the
Cys 2 -
Cys 10
disulfide bond is important for the stability but not for the folding mechanism of
ribonuclease T1 . -
pubmed
MDX4 - 4210 and Cosmesil facial elastomers had the strongest bond to skin with the majority of adhesive tapes , whereas Silskin
II , Cosmesil
HC2 , and
RS 330T - RTV were the weakest . -
pubmed
Home - made excimer lasers are operating
today in the US -- the
rest of the country watches and waits . -
pubmed
In the
MGL line ,
pyridoxine associated or not with
magnesium ( groups 2 and 4 ) significantly reduced the mean number of
ulcers . -
pubmed
Quaternary structure dynamics and
carbon monoxide binding kinetics of
hemoglobin valency hybrids . -
pubmed
In addition to
spermidine , the
enzyme also catalyzed the acetylation of
spermine and
putrescine with Michaelis constants ( Km values ) of 97 , 12 , and 10 microM , respectively . -
pubmed
culbertsoni , indicates a role for the
enzyme in the production of
1,3 - diaminopropane , the major
polyamine found in the Acanthamoeba . -
pubmed
Fatty acid composition of plasma and red cell phospholipids was measured within 4 days of birth and at time of
ERG testing , and detailed record was kept of dietary intake . -
pubmed
A positive correlation was seen between scotopic ( rod ) b - wave implicit time on
ERG and
DHA in both plasma ( r = 0.733 , P = 0.001 ) and red cells ( r = 0.502 , P = 0.04 ) . -
pubmed
A positive correlation was seen between
arachidonic acid and rod
ERG amplitude ( r = 0.565 , P = 0.022 ) which may reflect the higher
AA /
DHA ratio in the developing retina . -
pubmed
The MESAM
IV system measures arterial
oxygen saturation ( Sa , O2 ) , heart rate , snoring sounds and body position , and allows both automatic and manual scoring of the recordings . -
pubmed
Stereochemistry of oxygenation of
linoleic acid catalyzed by
prostaglandin - endoperoxide H synthase-2 . -
pubmed
The stereochemistry of the
hydrogen which was removed from C-11 during the conversion of
linoleic acid into
hydroxy acids in the presence of
PGHS-1 or
PGHS-2 was determined by incubation of
[ ( 11R )- 2H ]- and
[ ( 11S )- 2H ] linoleic acids followed by mass spectrometric analysis of the isotope contents of the individual
hydroxy acid isomers . -
pubmed
Both
enzymes were found to catalyze oxygenations which involved stereospecific removal of the
( 11S ) hydrogen and retention of the
( 11R ) hydrogen . -
pubmed
It is concluded that the initial steps of the
PGHS-2- and PGHS-1 - catalyzed oxygenations proceed with identical stereochemistry and involve stereospecific removal of the
pro - S hydrogen from the
omega 8 - methylene group of the substrate . -
pubmed
We were not able to characterize a
Ca2 + stimulated
ATPase , whereas 57 and 15 % of the total
ATPase activity were sensitive to
Mg2 + in the oviductal and endometrial fractions , respectively . -
pubmed
Increasing the preincubation time to 30 min , the
Mg2 + ATPase in the endometrial fraction was significantly inhibited by all four pesticides at 64 microM , but in the oviductal fraction only at 64 microM
o , p ''- DDT . -
pubmed
The
insulin suppression test consisted of a constant infusion of
glucose ,
insulin and
somatostatin for 150 min ;
insulin resistance was estimated by determining the steady - state plasma
glucose concentrations during the last 60 minutes of the test . -
pubmed
These results have also provided in vivo evidence that
DAG -
PKC activation could be responsible for the
hyperglycemia - induced
vascular dysfunctions in
diabetes . -
pubmed
Based on the demand for
cholesterol for membrane formation , we determined the ability of
low - density lipoprotein (
LDL ) to support proliferation in lymphocytes bearing different
LDL receptor mutations , which were treated " in vitro " with
lovastatin to inhibit endogenous
cholesterol synthesis . -
pubmed
Flow cytometric analysis with
1,1 '- dioctadecyl-3,3,3,3 '- tetramethylindocarbocyanineperchlorate (
Dil)-LDL showed normal
LDL binding but defective internalization in lymphocytes from case 1 , whereas in lymphocytes from case 2 both
LDL binding and internalization were affected . -
pubmed
No methylated or demethylated products of
DMA were detected in blood or any organ up to 8 h
post - exposure . -
pubmed
In this report , we demonstrate that
Us9 - promoted transneuronal spread in vivo is dependent on a conserved
acidic motif previously shown to be essential for the maintenance of
Us9 in the TGN region and recycling from the plasma membrane . -
pubmed
Mutant viruses with the
acidic motif deleted have an anterograde spread defect indistinguishable from that of
Us9 null viruses . -
pubmed
The compounds have been tested for their inhibitory effects on
Epstein - Barr virus early antigen (
EBV - EA ) activation induced by
12 - O - tetradecanoylphorbol-13 - acetate (
TPA ) , as a test for potential
cancer chemopreventive agents . -
pubmed
However , altering two
amino acid residues in the
EII ' enzyme enhanced the activity of
EII ' to the same level as that of the
EII enzyme . -
pubmed
The deduced
amino acid sequences from eight regions of
pOAD2 had significant similarity with the sequences of gene products such as
oppA - F ( encoding
oligopeptide permease ) ,
ftsX ( filamentation temperature sensitivity ) ,
penDE (
isopenicillin N - acyltransferase ) and
rep ( plasmid replication ) . -
pubmed
The data indicate that ( a ) both
c - Fos and
p53 are involved in cellular protection against the clastogenic effect of genotoxic agents , ( b )
p53 is not required for induction of apoptosis by UV light and
MMS , but rather prevents fibroblasts from undergoing apoptotic cell
death upon DNA damage , and ( c )
c - Fos and
p53 seem to act independently in determining genotoxic resistance , which is hypothesized to be achieved by impaired DNA repair or differential cell cycle check point control . -
pubmed
Furthermore , mechanisms such as direct inhibition of
oxygen free radical formation , abrogation of neutrophilic infiltration and reduced up - regulation of
adhesion molecules have also been documented in these studies . -
pubmed
The
cytochrome P4503A4 inhibitor
clarithromycin increases the plasma concentrations and effects of
repaglinide . -
pubmed
Plasma
repaglinide ,
serum insulin , and blood
glucose concentrations were measured up to 7 hours . -
pubmed
Clarithromycin increased the mean incremental area under the concentration - time curve from 0 to 3 hours of serum
insulin by 51 % ( P & lt;.05 ) and the maximum increase in the serum
insulin concentration by 61 % ( P & lt;.01 ) compared with placebo . -
pubmed
CONCLUSIONS : Even low doses of the
cytochrome P4503A4 (
CYP3A4 ) inhibitor
clarithromycin increase the plasma concentrations and effects of
repaglinide . -
pubmed
Concomitant use of
clarithromycin or other potent inhibitors of
CYP3A4 with
repaglinide may enhance its blood
glucose - lowering effect and increase the risk of
hypoglycemia . -
pubmed
Administration of an AA metabolism inhibitor , however , only blocked the stimulatory effect of
sGnRH without affecting
cGnRH - II - induced meiosis . -
pubmed
As observed previously , in the presence of
GtH ,
sGnRH was found to inhibit
GtH - induced resumption of meiosis and
testosterone production , whereas
cGnRH - II was without effect . -
pubmed
Platelet - derived growth factor activates production of reactive
oxygen species by
NAD ( P ) H oxidase in smooth muscle cells through
Gi1,2 . -
pubmed
Recent findings indicate that
platelet - derived growth factor (
PDGF ) plays a role in the generation of reactive
oxygen species ( ROS ) as second messengers in smooth muscle cells ( SMC ) . -
pubmed
ROS release was completely inhibited by the
Gi protein inhibitor
PTX as well as an antibody against Galphai1,2 , however , not by antibodies against
Galphai3 / 0 , Gas , and
Gbeta1beta2 . -
pubmed
Immunoaffinity chromatography demonstrated coupling of Galphai1,2 to the
PDGF a - receptor , which , after preincubation of the SMC membranes with
PDGF , was increased in the absence of GTPgammaS but decreased in the presence of GTPgammaS and prevented by
PTX treatment . -
pubmed
Analyses of both discrete traits (
type 2 diabetes and the composite trait of
type 2 diabetes and/or
impaired glucose homeostasis [
IGH ] ) , and quantitative traits (
glucose levels during a 75 - g oral
glucose challenge , designated
glucose 0 - 180 and HbA(1c ) ) were performed . -
pubmed
These results suggest that the antibodies prepared against omega -
CTX GVIA and B1Nt in this work have high selectivity for their
antigen . -
pubmed
Gastric intramucosal pH ( pHi ) ,
plasma endotoxin in portal vein , and plasma
D - lactic acid were measured and blood samples were cultured at different time intervals after
trauma . -
pubmed
Using
urea denaturation ,
coat protein monomers are shown to be marginally stable . -
pubmed
The reversible folding of
coat protein follows a three - state model , N if I if U , with an intermediate exhibiting most of the
tryptophan fluorescence of the folded state , but little secondary structure . -
pubmed
The specific binding of
L - tryptophan to
serum albumin . -
pubmed
Purification of native
TSH receptor has allowed to study directly and to characterize the autoantibodies directed against this
antigen . -
pubmed
The use of
triisopropylsilyl - oxymethyl (
TOM ) in the synthesis of
anti - telomerase 2 - 5A antisense compound
RBI 011 . -
pubmed
The 2 - 5A antisense compound
RBI 011 targeting
telomerase RNA was synthesized using the
triisopropylsilyl - oxymethyl (
TOM ) group for the
3 '- hydroxyl protection of 2',5'-linked RNA . -
pubmed
In the final steps of glycogenolysis , intracellular
glucose 6 - phosphate (
Glc-6 - P ) is transported into the endoplasmic reticulum where it is hydrolyzed to
glucose by
glucose-6 - phosphatase (
Glc-6 - Pase ) . -
pubmed
Although the majority of body glycogen is stored in the muscle , the current dogma holds that
Glc-6 - Pase ( now named
Glc-6 - Pase - alpha ) is expressed only in the liver , kidney , and intestine , implying that muscle glycogen can not contribute to interprandial blood
glucose homeostasis . -
pubmed
Ten years ago , the linkage between mutations in the gene coding for the antioxidant enzyme
Cu ,
Zn superoxide dismutase (
SOD1 ) and the
neurodegenerative disease known as
familial amyotrophic lateral sclerosis (
FALS ) was established . -
pubmed
Increased
glutathione (
gamma - glutamylcysteinylglycine ;
GSH ) conjugation ( inactivation ) of alkylating anticancer drugs due to overexpression of cytosolic
glutathione S - transferase (
GST ) is believed to be an important mechanism in
tumor cell resistance to alkylating agents . -
pubmed
In our experiments , a combination of lipid chromatography / electrospray ionization mass spectrometry ( LC / ESI /
MS ) was employed for structural characterization of the resulting conjugates between
CHB and
GSH . -
pubmed
We showed that microsomal
GST was activated by
CHB in a concentration and time dependent manner . -
pubmed
Microsomal
GST which was stimulated approximately two - fold with
CHB had a stronger catalytic effect . -
pubmed
Thus , microsomal
GST may play a potential role in the metabolism of
CHB in biological membranes , and in the development of
ADR . -
pubmed
Our results show that survival factor
NT-3 acts as a short - distance axon guidance
molecule for muscle sensory afferents as they approach their proper targets . -
pubmed
SNP - stimulated
( 18 ) F - FDG uptake was abolished by cotreatment with
cycloheximide , the
tyrosine kinase inhibitor
genistein , the
phosphatidylinositol-3 kinase (
PI3 K ) inhibitor
wortmannin , or the
protein kinase C inhibitor
staurosporine . -
pubmed
CONCLUSION :
NO stimulates
( 18 ) F - FDG uptake in HUVECs through an increase in
GLUT1 expression and
hexokinase activity , which appears to involve both
protein kinase C and
PI3 K pathways . -
pubmed
Using semiquantitative reverse transcription - PCR , the genes of the
gal operon were shown to be expressed more during growth in
iron medium than in growth in
sulfur medium . -
pubmed
RESULTS : While many old drugs are truly antiquated , medications such as typical antipsychotics , tricyclic and
monoamine oxidase antidepressants , and
lithium should remain integral parts of a psychopharmacology teaching program . -
pubmed
Insulin sensitivity derived from oral
glucose tolerance testing in athletes : disagreement between available indices . -
pubmed
The aims of the present study were to determine whether available " fasting " and oral
glucose tolerance test - derived
insulin sensitivity indices could effectively discriminate between individuals with higher than normal
insulin sensitivity , and whether they would all provide similar information in clinical practice . -
pubmed
Besides
glucose and
insulin responses , a total of nine such indices were computed . -
pubmed
Fasting as well as
post - load
glucose concentrations were similar in the three groups , while basal plasma
insulin and the insulinaemic response to
glucose were both higher in untrained individuals ( at P & lt ; 0.05 and P & lt ; 0.02 , respectively ) . -
pubmed
Apparently , the various
insulin sensitivity indices examined provided different quantitative and qualitative information , despite
insulin action being greater in both groups of athletes relative to controls , as reflected by their similar
glucose tolerance with lower
insulin concentrations . -
pubmed
Cu ,
Zn - superoxide dismutase is an intracellular catalyst for the
H ( 2 ) O ( 2 )-dependent oxidation of
dichlorodihydrofluorescein . -
pubmed
We compared the
peroxidase activity of
Cu ,
Zn - superoxide dismutase (
CuZnSOD ) ,
cytochrome c ,
horseradish peroxidase (
HRP ) ,
Cu ( 2 + ) , and
Fe ( 3 + ) under various condi - tions to identify an intracellular catalyst . -
pubmed
Enormous increase by
bicarbonate in the rate of
DCFH ( 2 ) oxidation distinguished
CuZnSOD from
cytochrome c and
HRP . -
pubmed
Cyanide inhibited the reaction catalyzed by
CuZnSOD but accelerated that by
Cu ( 2 + ) and
Fe ( 3 + ) . -
pubmed
We propose that
CuZnSOD is a potential intracellular catalyst for the
H ( 2 ) O ( 2 )-dependent oxidation of
DCFH ( 2 ) . -
pubmed
The addition of cross - linked murein ,
disaccharides linked to interconnected wall peptides , blocked
GFP -
CWT binding to staphylococci , whereas murein monomers or
lysostaphin - solubilized cell wall fragments did not . -
pubmed
A
tagO mutant
strain , defective in the synthesis of
polyribitol wall
teichoic acid attached to the cell wall envelope , displayed increased
GFP - CWT binding . -
pubmed
In contrast , a
femAB mutation , reducing both the amount and the length of peptidoglycan cross - linking (
monoglycine cross bridges ) , showed a dramatic reduction in
GFP -
CWT binding . -
pubmed
Pretreatment with
phenoxybenzamine , an
alpha adrenoceptor blocking agent , resulted in a small but statistically significant attenuation in the response of SCBF to brushing . -
pubmed
However , pretreatment with
propranolol , a
beta adrenoceptor blocking agent , or
atropine , a
muscarinic cholinergic receptor blocking agent , produced no such effect . -
pubmed
In
CT investigation irregular bone thickenings of anterior and lateral walls of maxillary
sinus were visible . -
pubmed
This process is notably exemplified by the discovery of the modified
betulinic acid derivative ,
DSB [
PA-457 ] , which is currently in Phase
II clinical trial and is the first - in - class HIV maturation inhibitor ( MI ) . -
pubmed
In contrast , addition of females injected with
Ovaprim ( to induce an
LH surge and ovulation ) increased male
LH concentrations at all sample times ( 5 , 9 , 13 , and 17h ) following female addition and increased milt volumes at all but the first ( 5h ) sample time . -
pubmed
Males also increased
LH and milt 9h after addition of females injected with the goldfish pheromonal
steroid 4 - pregnen-17,20beta - diol-3 - one ( 17,20betaP ) , suggesting that similar responses to ovaprim - injected females were due , at least in part , to release of preovulatory pheromonal
steroid(s ) . -
pubmed
Recent work has identified a role for
metabotropic glutamate receptor subtype 5 (
mGlu ( 5 ) ) in the discriminative stimulus properties of investigator - administered
ethanol . -
pubmed
The purpose of this study was to determine if
mGlu ( 5 ) receptors modulate the discriminative stimulus properties of self - administered
ethanol . -
pubmed
Results show that the
mGlu ( 5 ) receptor antagonist
6 - Methyl-2 -( phenylethynyl ) pyridine (
MPEP ; 10 mg / kg ) inhibited the discriminative stimulus properties of consumed
ethanol during a self - administration test session . -
pubmed
These results indicate that
mGlu ( 5 ) receptors are involved in the expression of the discriminative stimulus properties of self - administered
ethanol . -
pubmed
OBJECTIVES :
Methionine synthase reductase (
MTRR ) and
betaine - homocysteine S - methyltransferase (
BHMT ) are two enzymes that regulate
homocysteine metabolism . -
pubmed
We further evaluated whether
homocysteine levels differed between cases and controls for
MTRR and
BHMT genotypes . -
pubmed
The
etx gene on these plasmids was insertionally inactivated using a
chloramphenicol resistance cassette to phenotypically tag the plasmid . -
pubmed
The synthesized
1,8 - naphthyridine-3 - carboxamides were also evaluated for anti - inflammatory and myeloprotective activities , indicated by modulation in
cytokine and
chemokine levels secreted by dendritic cells . -
pubmed
After deletion of the central anaerobic fermentation genes (
ldhA ,
adhE ,
ackA ) , the pathway for
malate and
succinate production remained as the primary route for the regeneration of
NAD + . -
pubmed
Under anaerobic conditions ,
ATP production for growth was obligately coupled to
malate dehydrogenase and
fumarate reductase by the requirement for
NADH oxidation . -
pubmed
The best
succinate biocatalysts , strains KJ060(
ldhA ,
adhE ,
ackA ,
focA ,
pflB ) and KJ073(
ldhA ,
adhE ,
ackA ,
focA ,
pflB ,
mgsA ,
poxB ) , produce 622 - 733 mM of
succinate with molar yields of 1.2 - 1.6 per mole of metabolized
glucose . -
pubmed
The best
malate biocatalyst , strain KJ071(
ldhA ,
adhE ,
ackA ,
focA ,
pflB ,
mgsA ) , produced 516 mM
malate with molar yields of 1.4 per mole of
glucose metabolized . -
pubmed
Angiotensin - converting enzyme inhibitors ,
angiotensin II receptor blockers , and
statins have renoprotective effects . -
pubmed
Only
captopril consistently improved these abnormalities and reduced the cortical expression of several proinflammatory and profibrotic factors including
transforming growth factor - beta (
TGF - beta ) . -
pubmed
These effects were independent of blood pressure , accompanied by increased urine
N - acetylseryl - aspartyl - lysyl - proline (
Ac - SDKP ) levels , and the restoration of renal
angiotensin - converting enzyme and
angiotensin II to baseline levels . -
pubmed
Captopril or Ac - SDKP suppressed
angiotensin II - induced
MAPK activation and
TGF - beta secretion . -
pubmed
Angiotensin - converting enzyme inhibition confers renoprotection in
adriamycin nephropathy by reducing intrarenal
angiotensin II and augmenting Ac - SDKP expression that together attenuate
MAPK signaling and its downstream proinflammatory and fibrogenic properties . -
pubmed
The degree of alkylation ranged from
methyl (
C1 ) to
heptyl (
C7 ) and represents the types of APs present in produced water . -
pubmed
Independent of
alkyl homologue , the
glucuronide conjugated APs were the most abundant metabolites ( 73 - 100 % ) , whereas
sulfates ,
glucosides and unchanged compounds were excreted in amounts of 0 - 21 % , 0 - 6.1 % and 0 -
6.3 % , respectively . -
pubmed
PURPOSE :
Romidepsin (
FK228 ,
depsipeptide ,
FR901228 ) , a unique cyclic depsipeptide with a
histone deacetylase inhibitor (
HDACI ) activity , is a potential
cancer therapeutic agent and currently under clinical trials for several types of
cancer . -
pubmed
Our data indicate that
DMCA can potentiate the
HDACI activity of
romidepsin and other biological activities , such as cell cycle arrest and apoptosis ; these effects were accompanied with the expression of various key cell cycle regulators in different
bladder cancer cells . -
pubmed
FT - IR , FT - Raman spectra and ab initio
HF and DFT calculations of
4 - N , N '- dimethylamino pyridine . -
pubmed
Thyroid tests at birth , age at
TSH normalization and average thyroid tests under
L - T4 treatment during the 1st yr did not differ between sexes in any of the groups . -
pubmed
In both cell lines ,
MI - ER1 alpha interacted with
ER alpha in the presence and absence of
oestrogen , but the interaction was stronger in the absence of ligand . -
pubmed
17 - AAG sensitized
malignant glioma cells to
death - receptor mediated apoptosis . -
pubmed
17 - AAG is a selective
HSP90 - inhibitor that exhibited therapeutic activity in
cancer . -
pubmed
Treatment with subtoxic doses of
17 - AAG in combination with
tumor necrosis factor - related apoptosis - inducing ligand (
TRAIL ) induces rapid apoptosis in
TRAIL - resistant
glioma cells , suggesting that this combined treatment may offer an attractive strategy for treating
gliomas . -
pubmed
17 - AAG treatment down - regulated
survivin through proteasomal degradation . -
pubmed
In addition , over - expression of
survivin attenuated
cytotoxicity induced by the combination of
17 - AAG and
TRAIL . -
pubmed
Cytochrome P450 monooxygenase appears to be involve in the first step of
1,2,4 - TCB degradation , as evidenced by marked inhibition of both dechlorination and degradation of
1,2,4 - TCB in the presence of the known
cyt P450 inhibitors
1 - aminobenzotriazole and
piperonyl butoxide . -
pubmed
Based on these results , a degradation pathway of
1,2,4 - TCB through
cyt P450 monooxygenase and
epoxide hydrolase was proposed . -
pubmed
The neutron transmutation doping (
NTD ) method was applied to the initially p - type
silicon in order to extend the
NTD applications at HANARO . -
pubmed
Our goal was to investigate the effects of exposure to
nicotine on maternal , fetal , and neonatal
IGF - II processing . -
pubmed
Nicotine exposure prevented the decrease in maternal
IGF - II processing seen in controls with advancing gestation . -
pubmed
However , there was no influence of
nicotine on fetal levels of
IGF - II . -
pubmed
Postnatally ( postnatal day [
PND ] 21 ) , pups exposed to
nicotine in utero had decreased levels of big
IGF - II . -
pubmed
Our results show , for the first time , that
nicotine exposure prevents the decrease of
IGF - II processing in the maternal compartment . -
pubmed
Sequencing of the complete genome of one of these variants identified a single point mutation that resulted in a substitution of a conserved
asparagine by
histidine at position 498 of the
haemagglutinin - neuraminidase protein , although this mutation was not present in all rapidly growing variants . -
pubmed
3 - Bromoacetamino-4 - methoxy - benzoylurea ( F13 ) is a
benzoylurea derivative selected from the library of small molecule
tubulin ligands . -
pubmed
This was found to occur through the upregulation of the reversion - inducing
cysteine - rich protein with Kazal motifs (
RECK ) , a membrane - anchored inhibitor of
MMPs , which acts by reducing
ERK1 / 2 phosphorylation . -
pubmed
Data collection will take place during the Physical Education ( PE ) lessons and on school premises and will include : anthropometrical variables ( height , weight , waist and hip circumferences , skinfold thickness at two sites ) , physiological responses ( blood pressure , aerobic fitness , heart rate ( HR ) response , vertical jump performance , 10 - metre ( m ) sprint , 50 - m sprint and 505 - agility test ) , diet ( self - reported seven - day food diary ) , physical activity ( Physical Activity Questionnaire for Adolescents ( PAQ - A ) ) and blood tests ( fasting
glucose ,
insulin , total
cholesterol (
TC ) ,
high - density lipoprotein (
HDL ) , high - sensitivity
C - reactive protein (
hs - CRP ) ,
fibrinogen (
Fg ) ,
interleukin-6 (
IL-6 ) ,
adiponectin ( high molecular weight ) ,
triglyceride and
plasminogen activator inhibitor-1 (
PAI-1 ) . -
pubmed
The diagnosis of
MNGIE disease can be established in a proband by detection of one of the following : ( 1 ) biallelic pathogenic variants in
TYMP ( formerly known as
ECGF1 ) ; ( 2 ) markedly reduced levels of
thymidine phosphorylase enzyme activity ; or ( 3 ) elevated plasma concentrations of
thymidine and
deoxyuridine . -
pubmed
PR -
Set7 - mediated monomethylation of
histone H4 lysine 20 at specific genomic regions induces transcriptional repression . -
pubmed
Lipoprotein lipase (
LPL)-mediated lipolysis of
triglycerides is the first and rate - limiting step in chylomicron / very low density lipoprotein clearance at the luminal surface of the capillaries . -
pubmed
Moreover ,
ANGPTL3 enhances
LPL cleavage in the presence of either
heparan sulfate proteoglycans or
glycosylphosphatidylinositol - anchored
high density lipoprotein - binding protein 1 (
GPIHBP1 ) . -
pubmed
In this study , using a placental explant model , we investigated whether different
oxygen environments induced abnormal shedding of trophoblastic debris or secretion of
cytokines from the placenta . -
pubmed
There was also no difference in the levels of activated
caspases in trophoblasts shed from explants cultured in these two
oxygen environments nor was there a significant difference in the endothelial cell responses to trophoblasts shed from explants cultured in 1 % or 8 %
oxygen . -
pubmed
Similarly , there was no significant change in the secretion of nine
cytokines into the conditioned medium from explants cultured in 1 % or 8 %
oxygen . -
pubmed
RESULTS : The mixed venous
oxygen saturation ( SvO(2 ) ) was lower through
T2 - T4 compared to the baseline value in both groups , while the degree of the decrease was significantly less in group M than that in group C. The other hemodynamic variables , the operative data and the postoperative outcomes were similar between the two groups . -
pubmed
Matrix - assisted laser desorption / ionization ( MALDI ) was performed on an external ion source Fourier transform ion cyclotron resonance mass spectrometer ( FTICR -
MS ) to analyze the block length distributions of triblock polymers of
poly ( oxypropylene ) and
poly ( oxyethylene ) . -
pubmed
CBD elicits an endoplasmic reticulum (
ER ) stress response , characterized by changes in
ER morphology and the initiation of
RNA - dependent protein kinase - like ER kinase- , activating
transcription factor-6- , and
inositol - requiring ER - to - nucleus signal kinase-1 (
IRE1)-mediated signaling cascades . -
pubmed
Furthermore ,
CBD induces downstream activation of the pro - apoptotic
IRE1 /
ASK1 /
c - Jun N - terminal kinase pathway , leading to HSC
death . -
pubmed
Finally , we provide evidence that the elevated basal level of
ER stress in activated HSCs has a role in their susceptibility to the pro - apoptotic effect of
CBD . -
pubmed
DeltaywdL spores are unable to germinate in response to
CaDPA , and have altered germination properties , a phenotype that confirms the expected defect in localization of the cortex lytic enzyme
CwlJ in the coat . -
pubmed
The repair of
cis - syn cyclobutane pyrimidine dimers (
CPDs ) can be initiated via the base excision repair ( BER ) pathway , utilizing
pyrimidine dimer - specific
DNA glycosylase /
lyase enzymes (
pdgs ) . -
pubmed
High purity recombinant proteins rEhCBP30 , rEhCBP30 -
CHP and
rEhCBP30 - ATP with N - terminal
His tag were obtained by single step affinity purification . -
pubmed
After a gap of almost 60 years following the development of
warfarin , 2 new categories of oral anticoagulant agents have been approved for clinical use - the direct
thrombin inhibitors and
factor Xa inhibitors . -
pubmed
There is a difference between immature and adult brain in an appearance of
withdrawal symptom after administration of the partial agonist of
benzodiazepine receptors Ro 19 - 8022 . -
pubmed
Lysophosphatidic acid promotes cell migration through
STIM1- and
Orai1 - mediated
Ca2 +( i ) mobilization and
NFAT2 activation . -
pubmed
Here we investigated the involvement of agonist - induced
Ca ( 2 + ) entry and
STIM1 and
Orai1 proteins in regulating nuclear factor of activated T cell (
NFAT ) signaling and
LPA - induced keratinocyte cell motility . -
pubmed
Store - operated
Ca ( 2 + ) entry ( SOCE ) was blocked by the
SOCE inhibitor
diethylstilbestrol (
DES ) ,
STIM1 silencing using RNA interference ( RNAi ) , and expression of dominant / negative
Orai1(R91W ) . -
pubmed
LPA induced significant
NFAT activation as monitored by nuclear translocation of
green fluorescent protein - tagged
NFAT2 and a luciferase reporter assay , which was impaired by
DES , expression of
Orai1(R91W ) , and inhibition of
calcineurin using
cyclosporin A (
CsA ) . -
pubmed
By using chemotactic migration assays ,
LPA - induced cell motility was significantly impaired by
STIM1 ,
CsA , and
NFAT2 knockdown using RNAi . -
pubmed
These data indicate that in conditions relevant to epidermal
wound healing ,
LPA induces SOCE and
NFAT activation through
Orai1 channels and promotes cell migration through a
calcineurin /
NFAT2 - dependent pathway . -
pubmed
The effect of
tamoxifen was considered a clinical complete response (
CR ) . -
pubmed
Telmisartan administration resulted in significant suppression in
tumor necrosis factor - alpha expression and
nuclear factor - kappaB phosphorylation . -
pubmed
The two - component system (
TCS )
KdpD /
KdpE , extensively studied for its regulatory role in
potassium ( K(+ ) ) transport , has more recently been identified as an adaptive regulator involved in the virulence and intracellular survival of pathogenic bacteria , including Staphylococcus aureus , entero - haemorrhagic Escherichia coli , Salmonella typhimurium , Yersinia pestis , Francisella species , Photorhabdus asymbiotica , and mycobacteria . -
pubmed
Here we employ
STM to study individual
tris -( phenylpyridine )- iridium complexes on a
C60 monolayer , and investigate the influence of their electronic structure on the plasmon excitation between the
Ag ( 111 ) substrate and an
Ag - covered
Au tip . -
pubmed
Prospective and
post - hoc analyses from these phase III studies show
rasagiline monotherapy significantly improved
tremor symptoms in early
PD , independent of disease duration , compared with placebo . -
pubmed
A six - week treatment of weekly intraperitoneal injections of
PNP ( 50 mg / kg ) resulted in severe damage to the seminiferous tubules , a remarkable increase in both
hydroxyl radical and
malondiadehyde production , and notably decreased
glutathione peroxidase and
superoxide dismutase activities . -
pubmed
Moreover ,
PNP treatment induced germ cell apoptosis , inhibited
Bcl - xl expression , and then activated
Bax expression and the
caspase-3 enzyme . -
pubmed
However , simultaneous supplementation with
quercetin ( 75 mg / kg ) attenuated the
toxicity induced by
PNP through renewal of the antioxidant enzyme 's status , alleviating apoptosis by regulating the expressions of
Bax and
Bcl - xl ,
XBP-1 and
HO-1mRNAs , and the regulation of
caspase-3 activity . -
pubmed
This study compared five technology configurations : 1 - conventional AD with
CHP , 2 - Thermal Hydrolysis Process (
THP ) AD with
CHP , 3 -
THP AD with bio -
methane grid injection , 4 -
THP AD with
CHP followed by drying of digested sludge for solid fuel production ,
5 - THP AD followed by drying , pyrolysis of the digested sludge and use of the both the biogas and the pyrolysis gas in a
CHP . -
pubmed
Towards achieving this goal , several researchers have carried out studies in various cell lines and in vivo and have deciphered that
fucoxanthin exerts its anti - proliferative and
cancer preventing influence via different molecules and pathways including the
Bcl-2 proteins ,
MAPK ,
NFkappaB ,
Caspases ,
GADD45 , and several other molecules that are involved in either cell cycle arrest , apoptosis , or
metastasis . -
pubmed
The
amino acid sequence of the protein corresponded to that of
albumin . -
pubmed
Toxicity and quality of life after
choline - PET /
CT directed salvage lymph node dissection and adjuvant radiotherapy in
nodal recurrent
prostate cancer . -
pubmed
BACKGROUND : In a previous study we demonstrated that , based on
11C / 18 F - choline positron emission tomography - computerized - tomography as a diagnostic tool , salvage lymph node dissection ( LND ) plus adjuvant radiotherapy (
ART ) is feasible for treatment of pelvic / retroperitoneal
nodal recurrence of
prostate cancer (
PCa ) . -
pubmed
In general ,
TRPs are nonselective cation channels which are
Ca ( 2 + ) permeable . -
pubmed
Fibroin 1 is dominated by
glycine -
alanine motifs , and lacks sequence motifs associated with
orb - weaver
MaSps . -
pubmed
We hypothesize
fibroin 2 is a piriform or aciniform silk protein , based on
amino acid composition , spigot morphology , and phylogenetic analyses . -
pubmed
To overcome this immediate lethality , we generated a
doxycycline - responsive tTA -
DNMT1 * rescue line and readily obtained homozygous
DNMT1 - mutant lines . -
pubmed
However ,
doxycycline - mediated repression of exogenous
DNMT1 * initiates rapid , global loss of DNA methylation , followed by extensive cell
death . -
pubmed
Characterization of
JNJ-42847922 , a Selective
Orexin-2 Receptor Antagonist , as a Clinical Candidate for the Treatment of
Insomnia . -
pubmed
Here , we characterized
JNJ-42847922 (
[ 5 -( 4,6 - dimethyl - pyrimidin-2 - yl )- hexahydro - pyrrolo [ 3,4 - c ] pyrrol-2 - yl ]-( 2 - fluoro-6 -[ 1,2,3 ] triazol-2 - yl - phenyl )- methanone ) , a high - affinity / potent
OX2R antagonist . -
pubmed
JNJ-42847922 had an approximate 2 - log selectivity ratio versus the
human orexin-1 receptor . -
pubmed
Associations between the dysregulation of the hypothalamic - pituitary - adrenal (
HPA ) axis and these disorders have been recognized in the scientific literature , specifically in regard to
cortisol levels , as well as changes in pro- and anti - inflammatory
cytokines . -
pubmed
Identification of a
resveratrol tetramer as a potent inhibitor of
hepatitis C virus helicase . -
pubmed
Combined treatment of
vitisin B with an
NS5B polymerase inhibitor (
sofosbuvir ) exhibited a synergistic or at least additive antiviral activity . -
pubmed
We confirmed a direct binding between
vitisin B and a purified
NS3 helicase in vitro . -
pubmed
Vitisin B was a potent inhibitor of a
HCV NS3 helicase ( IC50 = 3 nM ) . -
pubmed
Conclusion and Implications
Vitisin B is one of the most potent
HCV helicase inhibitors identified so far . -
pubmed
scoparia and to determine the relationship of
EPSPS gene amplification with the level of
glyphosate resistance . -
pubmed
In the absence of
glyphosate , no differences in growth and reproductive parameters were evident between the tested
GR and SUS inbred lines , across an intraspecific competition gradient ( 1 to 170 plants m-2 ) . -
pubmed
scoparia plants with 2 to 4 copies of the
EPSPS gene survived the field - use rate ( 870 g ha-1 ) of
glyphosate , but failed to survive the 4,350 g ha-1 rate of
glyphosate ( five - times the field - use rate ) . -
pubmed
In contrast ,
GR plants with 5 to 14
EPSPS gene copies survived the 4,350 g ha-1 of
glyphosate . -
pubmed
scoparia with 5 or more
EPSPS gene copies will most likely persist in field populations , irrespective of
glyphosate selection pressure . -
pubmed
Analysis of variance ( ANOVA ) and Tukey 's test were used to verify any differences in
creatinine levels and estimated clearance at 0 , 6 , 12 , 24 and 36 months , adjusting for the co - variables sex , skin color , age & gt;50 years ,
arterial hypertension ,
diabetes and the use of the
ritonavir - boosted
protease inhibitors (
PI / r )
lopinavir / r or
atazanavir / r. The software package STATISTICA 10 ( ) was used for statistical analysis . -
pubmed
Approximately 27
deaths could be avoided for every 1000 people receiving
NLT of
beta - adrenergic blocking agents , ACEIs , and ARBs . -
pubmed
Participants in the
NLT group reached maximal dose of
beta - adrenergic blocking agents in half the time compared with participants in usual care . -
pubmed
In one scenario , the loss of function of
lipoic acid synthase , an enzyme with
Fe - S cluster cofactor in mitochondria , impairs activity of various
lipoamide - dependent enzymes with drastic consequences for metabolism . -
pubmed
In a third scenario ,
tRNA modifications arising from action of the
cysteine desulfurase and/or
Fe - S cluster proteins are lost , which may lead to impaired protein synthesis . -
pubmed
METHODS : MATRICS Consensus Cognitive Battery (
MCCB ) performance was assessed in
CPD ( n = 41 ) and healthy subjects ( HS ; n = 41 ) in a double - blind , randomized cross - over design of acute ( single dose )
MEM ( placebo vs. 10 or 20 mg p.o . ) -
pubmed
In
CPD subjects , greater age , positive
MEM effects on PPI , and SNP rs1337697 ( within the
ionotropic NMDA receptor gene ,
GRIN3A ) predicted greater positive OCME with 20 mg
MEM . -
pubmed
First , elevated
GTSP1 expression in
SD patients might be the result of activation of
GTSP1 in response to exposure to
carcinogens . -
pubmed
Composed of ten subunits that add up to a molecular mass of about 500 kDa ,
TFIIH is also essential for
nucleotide excision repair . -
pubmed
Geological evidence suggests that after the Late Pleistocene closure of the Danakil - Red Sea connection , increased
post -
glacial groundwater availability caused the formation of a brackish paleo - lake flooding the entire region below the -50 m contour . -
pubmed
MTX concentrations were not significantly different in the brain or
CSF 6 hr after the intrathecal administration of
MTX . -
pubmed
However , compare to intravenous administration of
MTX , intravenous administration of both
FA and
MTX significantly decreased
MTX concentrations in the brains and
CSF . -
pubmed
These results suggest that
FA inhibits the influx of
MTX into the brain and CSF , possibly by competing with
folate carriers , but has no effect on its efflux from these regions . -
pubmed
This paper presented the studies on effects of various concentrations of
BPA ( 0 , 0.25 mg kg-1 , 0.50 mg kg-1 , 1.00 mg kg-1 , 2.00 mg kg-1 ) and soil aertaiton ( aerobic and anaerobic ) on characteristics of paddy soil microbial communites by technology of qPCR ( fluorescence quantitative PCR ) and PCR - DGGE (
polymerase chain reaction - denaturing gradient gel electrophoresis ) . -
pubmed
Two - month treatment with
HCQ resulted in significant decrease in SLEDAI-2 K ( p & lt ; 0.001 ) , anti - dsDNA ( p & lt ; 0.001 ) ,
IL-1beta ( p = 0.003 ) ,
IL-6 ( p & lt ; 0.001 ) and
TNF - alpha ( p & lt ; 0.001 ) and a significant increase in
CH50 levels ( p = 0.012 ) . -
pubmed
Compounds 2 - 6 showed potent in vitro
pancreatic lipase inhibitory activity , suggesting their participation in the reductive effect of the herbs on
triglyceride absorption . -
pubmed
Pretreatment with
baicalin decreased the inflammatory response ; pretreatment with
baicalin and
berberine hydrochloride decreased the inflammatory response mediated by the
NF - kappaB signaling pathway . -
pubmed
Eco - friendly hydrogel composite beads based on crosslinked - carboxymethyl cellulose ( CMC ) and dextran sulfate (
DS ) embedded within network were prepared using ionotropic gelation in presence of
sodium n - dodecyl sulfate (
SDS ) as pore - forming template . -
pubmed
The effect of
DS on adsorption of composites for cationic dye
methylene blue (
MB ) was investigated by changing influencing factors : pH , adsorbent dosage , time contact , dye concentration , and temperature . -
pubmed
Further LC -
MS analysis confirmed the similarity of fungal
resveratrol with standard
resveratrol . -
pubmed
CLP - protein binding assay following proteome analysis reveals that
iturin A attached to
12 - oxophytodienoate reductase 2 involved in the
oxylipin biosynthetic process required for
jasmonic acid production which is implicated in induced systemic resistance ( ISR ) . -
pubmed
This study suggests specific elicitor action of individual
CLP , particularly
iturin A showed the most powerful in stimulating the ISR system in response to stresses in postharvest mandarins . -
pubmed
Both ligands have been previously developed as mimics of the tetrapeptide sequence
Asp1 - Trp2 - Val3 - Ile4 of the
cadherin adhesion arm , and have been shown to inhibit
E - cadherin - mediated adhesion in epithelial
ovarian cancer cells with millimolar potency . -
pubmed
Overall , these results will help the design of novel
cadherin inhibitors that might prevent the swap dimer formation by targeting both the
Trp2 binding pocket and the adhesive arm residues . -
pubmed
In contrast ,
AccMGST1 expression was inhibited when the antioxidant
vitamin C ( VC ) was fed to experimental bees . -
pubmed
Bromodomain and extraterminal domain inhibition synergizes with
WEE1 - inhibitor
AZD1775 effect by impairing nonhomologous end joining and enhancing DNA damage in
nonsmall cell lung cancer . -
pubmed
AZD1775 , an inhibitor of the
G2 - M
cell cycle checkpoint kinase WEE1 , induces DNA damage by promoting premature mitotic entry . -
pubmed
Thus , we hypothesized that
BET inhibition would increase
AZD1775 - induced
cytotoxicity by impairing DNA damage repair . -
pubmed
Two
BET inhibitors ,
JQ1 and
AZD5153 , increased and prolonged
AZD1775 - induced DNA double - strand breaks ( DSBs ) and concomitantly repressed genes related to nonhomologous end joining ( NHEJ ) , including
XRCC4 and
SHLD1 . -
pubmed
The objective of this study was to determine if combination of
polymyxin (
polymyxin B or
colistin ) with a nonantibiotic small molecule AR-12 , a
celecoxib derivative that is devoid of
cyclooxygenase 2 (
COX-2 ) inhibitory activities , can be an effective strategy against
polymyxin - resistant MDR bacteria . -
pubmed
While
NT - proBNP levels did not change significantly under treatment ( 2561 + - 2042 ng / l vs. 1938 + - 1524 ng / l , p = 0.20 ) ,
creatinine levels increased ( 1.14 + - 0.52 mg / dl vs. 1.35 + - 0.74 mg / dl , p = 0.002 ) . -
pubmed
Estrogen induction upregulated the expression of
GPAM and
AGPAT2 , downregulated the expression of
AGPAT3 ,
AGPAT9 ,
LPIN1 and
LPIN2 , and had no effect on the expression of the other genes . -
pubmed
This family comprises six members three of which ,
GATA4 , 5 , and 6 , are predominantly expressed in cardiac cells where they activate specific downstream gene targets via interactions with one another and with other TFs and signaling
molecules . -
pubmed
Glucose - Sensitive Nanoparticles Based On
Poly ( 3 - Acrylamidophenylboronic Acid - Block - N - Vinylcaprolactam ) For
Insulin Delivery . -
pubmed
Background : Compared with random copolymers , block copolymerization is easier to prepare for nanoparticles with core - shell structure , and they will have better
glucose sensitivity and higher
insulin loading . -
pubmed
Purpose : In our study ,
insulin - loaded
poly ( 3 - acrylamidophenylboronic acid - block - N - vinyl caprolactam ) p ( AAPBA - b - NVCL ) nanoparticles were successfully prepared and were
glucose - sensitive , which could effectively lower the blood
sugar levels within 72 hrs . -
pubmed
Meanwhile ,
insulin can be easily loaded by
p ( AAPBA - b - NVCL ) nanoparticles and an effective sustained release of
insulin was observed when the nanoparticles were placed in physiological saline . -
pubmed
Conclusion : Our research suggested that these
p ( AAPBA - b - NVCL ) nanoparticles might have the potential to be applied in a delivery system for
insulin or other hypoglycemic proteins . -
pubmed
Effect of
pemafibrate , a novel selective
peroxisome proliferator - activated receptor - alpha modulator (
SPPARMalpha ) , on urinary protein excretion in
IgA nephropathy with
hypertriglyceridemia . -
pubmed
Pemafibrate is a novel selective
peroxisome proliferator - activated receptor - alpha modulator and may be distinct from conventional fibrates in terms of its pharmacological activity and hepatic and renal safety . -
pubmed
METHODS : This was a retrospective study of adult commercial and Medicare Advantage with Part D ( MAPD ) enrollees with at least one claim for a
PAH - related medication from January 01 , 2012 to March 31 , 2017 , at least one medical claim with a
pulmonary hypertension diagnosis , and continuous health plan enrollment at least 6 months prior to and at least 12 months following the date of the first pharmacy claim for
PAH - related therapy ( index date ) . -
pubmed
From EELS measurements performed for 99.99 %
Al ,
Si wafer and 99.99 %
Fe , linear relationships were confirmed between the thickness of the
TEM thin foil and the ratio of the total intensity of EELS spectrum to the total intensity of zero - loss spectrum for all samples . -
pubmed
The
coil of the generator was adapted to easy handling and safe integration into the shelf of the
CO ( 2 ) incubator . -
pubmed
Functional changes ( acceleration of cell migration ) after
AMC exposure were statistically significant for the
MSC samples only . -
pubmed
Alteration of cell motility dynamics through
collagen fiber density in photopolymerized
polyethylene glycol hydrogels . -
pubmed
We demonstrated a method to encapsulate
collagen into
PEG hydrogel crosslinked via photopolymerization and studied the effect of fiber density on motility dynamics . -
pubmed
The changes in [
Ca2+]cys induced by
HCO3 - could be detected by an
aequorin - based
calcium imaging system . -
pubmed
In vitro stability of
PDMS - SB - UU was confirmed against
lipase and 30 %
H2O2 for 8 weeks , and
PDMS - SB - UU demonstrated significantly higher resistance to
fibrinogen adsorption and platelet deposition compared to control
PDMS . -
pubmed
After the treatment period , the histopathological examination of nasal and
sinus mucosa was performed and levels of
cytokines (
tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1beta (
IL-1beta ) ,
interleukin-6 (
IL-6 ) ) and oxidative stress in the blood , nasal mucosa , lungs and brain were also analyzed . -
pubmed
Quercetin administration diminished the exudate and degree of
inflammation in lamina propria of nasal and sinusal areas , parallel with the decreased secretion of
TNF - alpha ( 40.2 % reduction after the low dose of LPS , and 35.4 % reduction after the high dose of LPS ) and
IL-6 ( 21.4 % reduction after the low dose of LPS and 35.8 % reduction after the high dose of LPS ) . -
pubmed
In lungs ,
quercetin reduced
TNF - alpha ( 43.3 % ) and
IL-6 levels ( 24.5 % ) , and in the brain , the protective effect was noticed only on
TNF - alpha ( 46.5 % ) . -
pubmed
Genes that encoding the key enzyme in
carbon - related metabolism such as
Rubisco ,
AMY ,
PK ,
ME ,
CS ,
ACAT ,
KAS , and
DGAT were identified . -
pubmed
Cellular and molecular features of
EDC exposure : consequences for the
GnRH network . -
pubmed
This observation , together with our previous finding that mutant strains defective in
acyl - CoA synthetase I can not incorporate exogenous
fatty acid into cellular lipids but are able to degrade
fatty acid via beta - oxidation , indicates the presence of two functionally distinct long - chain
acyl - CoA pools in the cell -- i.e. -
pubmed
Unlike the wild - type and the revertant
strains as well as the mutants lacking
acyl - CoA synthetase II , the mutants defective in
acyl - CoA synthetase I do not exhibit the repression of
acetyl - CoA carboxylase [
acetyl - CoA : carbon - dioxide ligase (
ADP - forming ) ,
EC 6.4.1.2 ] by exogenous
fatty acid . -
pubmed
Measurement of the two long - chain
acyl - CoA pools with the aid of appropriate mutant
strains has indicated that the long - chain
acyl - CoA to be utilized for lipid synthesis , but not that to be degraded via beta - oxidation , is involved in the repression of
acetyl - CoA carboxylase . -
pubmed
Refolding and reactivation of liver
alcohol dehydrogenase after dissociation and denaturation in 6 M
guanidine hydrochloride . -
pubmed
Horse - liver alcohol dehydrogenase has been dissociated and denatured by 6 M
guanidinium hydrochloride . -
pubmed
The concentrations of the
enzyme , as well as the coenzyme (
NAD + ) , and
Zn2 + , affect the reactivation significantly , since high concentrations promote the formation of inactive aggregation products . -
pubmed
injection of either
clorgyline ( 0.5 , 1 or 5 mg / kg ) which selectively inhibits
Type A MAO or
deprenyl ( 0.5 , 5 or 10 mg / kg ) which selectively inhibits
Type B MAO . -
pubmed
Glucoprivation produced by 0.5 - -2 U / kg
insulin did not stimulate food intake although plasma
glucose fell to a mean of 49 mg/100 ml ( 43 % of control ) after 1 U / kg
insulin . -
pubmed
Pharmacological blockade of the action of
glutamate at the
N - methyl - D - aspartate ( NMDA ) receptor , ( the
glutamate receptor subtype principally involved in the
neurotoxic effects of the
amino acid ) provides a novel therapeutic approach to
cerebral ischaemia . -
pubmed
Recoverin is a recently discovered 26 kDa
calcium - binding protein , which activates
guanylate cyclase in retinal photoreceptors when the intracellular concentration of free
calcium drops upon photoexcitation . -
pubmed
Infusion of
ANF caused a progressive fall of plasma
aldosterone and
plasma renin activity . -
pubmed
Intrathecal perfusion with 50 and 500 microM
5 - HT also released a
nucleotide which eluted from the HPLC column at a retention time identical to that of
cyclic AMP standards , and was reduced following incubation with
cyclic AMP phosphodiesterase . -
pubmed
At
P2 and at
P7 , concentrations of
electron - dense material were observed in 0.5 - 0.7 % of the axon profiles in the medullary PT . -
pubmed
Our results show that a decondensed state of the NOR chromatin is necessary but not sufficient to permit
silver impregnation , allowing us to conclude that transcriptional
NOR activity in the previous interphase is additionally required for
silver staining . -
pubmed
Experiments were designed to analyze the effects of
endothelium - derived relaxing factor(s ) (
EDRF ; released basally or on stimulation with
acetylcholine ) and
nitric oxide (
NO ) on smooth muscle of coronary arteries of different diameter . -
pubmed
Because of similar
T2 NMR relaxation times , the water was doped with a paramagnetic ion ,
Mn+2 , to reduce its
T2 relaxation time . -
pubmed
Is
phospholipid a required cofactor for the activity of
mammalian signal peptidase ? -
pubmed
We have used the
calmodulin antagonists ,
trifluoperazine (
TFP ) and
calmidazolium , to study the potential role of this protein in the movement of
acetylcholine receptors (
AChRs ) to and from the myotube membrane , as well as in the formation of clusters of
AChRs within the plasma membrane . -
pubmed
An enantioselective high - performance liquid chromatographic method for
chloroquine and
desethylchloroquine was developed using a chiral
alpha 1 - acid glycoprotein column . -
pubmed
[ Relation between optic absorption spectrum and structure of
nitrosyl hemoglobin ] . -
pubmed
Norepinephrine and the
post - ejaculatory refractory period . -
pubmed
Administration of
norepinephrine synthesis inhibitors significantly increased both mount and intromission latencies and caused a dramatic increase in the length of the
post - ejaculatory refractory period . -
pubmed
, by the addition of
decoyinine , a specific inhibitor of
GMP synthetase . -
pubmed
Therefore , we studied the effect of
xenon inhalation on the
CT appearance of five
demyelinated plaques . -
pubmed
Therefore ,
xenon enhancement does not generally increase the sensitivity of
CT for detecting
cerebral demyelination . -
pubmed
The serological diagnosis of virus
hepatitis A and B depends on the demonstration of the various virus antigens (
HA - antigen ,
HBs - antigen ,
HBe - antigen ) and on their corresponding antibodies (
HA- ,
IgG- and
IgM - antibody ,
HBc - antibody ,
Hbe - antibody and
HBs - antibody ) . -
pubmed
Our observations suggest that
vitamin C stimulates the elaboration of
Ig M - type antibodies in particular . -
pubmed
Sugar meal analysis , using high - performance anion exchange ( HPAE ) chromatography with pulsed amperometric detection (
PAD ) and the cold
anthrone test , was used to demonstrate that significantly more sandflies fed on bean plants contaminated with aphids and honeydew than on uncontaminated beans . -
pubmed
Single -
amino - acid substitutions were made at several positions within the DNA binding domain of
p53 , and this set of
p53 point mutants were tested with DNA site variants for DNA binding . -
pubmed
Interestingly , mutant
Cys-277 to
Gln bound a consensus site as two and four monomers , as opposed to the wild - type
p53 fragment , which invariably binds this site as four monomers . -
pubmed
Stimulation of heparinized blood with 1 microM
formyl - methionyl - leucyl - phenylalanine (
FMLP ) resulted in the formation of & lt ; 30 pmol / ml plasma of
5 - lipoxygenase ( 5 - LO ) products . -
pubmed
The priming effect of
LPS was also observed using the complement fragment
C5a or
interleukin 8 as agonists . -
pubmed
Treatment of PMN with LPS strongly enhanced the release of
arachidonic acid after stimulation with
FMLP . -
pubmed
Involvement of
ryanodine receptors in
sphingosylphosphorylcholine - induced
calcium release from brain microsomes . -
pubmed
SPC has little effect on
[ 3H ] IP3 binding but enhances [
3H ]
ryanodine binding , as expected for an activator of
ryanodine receptors . -
pubmed
SPC - induced
Ca2 + release is inhibited by
ryanodine receptor blockers but not by selective blockers of
IP3 receptors . -
pubmed
We conclude that
SPC releases
Ca2 + from brain microsomes by activating
ryanodine receptors rather than
IP3 receptors . -
pubmed
Activation of an additional
SPC - sensitive pathway for releasing
Ca2 + is not precluded . -
pubmed
In this report , from the
nucleotide sequence , we demonstrate that
leukocidin and
gamma - hemolysin share the F or H gamma I component and the specificity of both toxins depends on this component . -
pubmed
A common target of
FK506 and
cyclosporin A is inhibition of activation of the
NFAT transcription factor , for which a specific binding region is present in the promoter of the IL-2 gene . -
pubmed
The isolation protocol included tissue homogenization in 0.4 M
HClO4 , centrifugation , solid - phase extraction through LiChroprep RP-18 material , chromatofocusing on polybuffer exchanger
PBE 94 - modified Sepharose and dialysis against water . -
pubmed
The
amino terminus of
mammalian nucleolin specifically recognizes
SV40 T - antigen type nuclear localization sequences . -
pubmed
coli
D - glyceraldehyde-3 - phosphate dehydrogenase modified by
2,3 - butanedione : manifestation of a pairwise of non - equivalence of active centers . -
pubmed
coli
d - glyceraldehyde-3 - phosphate dehydrogenase by an
arginine - specific reagent ,
2,3 - butanedione , stabilized the tetrametric enzyme in an asymmetric state , with only two of the four active centers able to catalyze oxidative phosphorylation of
D - glyceraldehyde-3 - phosphate . -
pubmed
Analogous results have been previously obtained with the
rabbit muscle D - glyceraldehyde dehydrogenase modified at a single
arginine residue per subunit ( Kuzminskaya , E.V. -
pubmed
coli
enzyme , activity loss upon
arginine modification never exceeded 80 - 82 % . -
pubmed
Determination of
phosphatidylserine ( PS ) in the outer leaflet of RBC by the PS - dependent
prothrombinase assay revealed that PS redistributed to the cell 's outer leaflet upon in vitro storage and vesiculation . -
pubmed
Inhibition of PS movement by oxidation of membrane
sulfhydryls with
pyridyldithioethylamine resulted in higher
prothrombinase levels and enhanced clearance of vesiculated RBC . -
pubmed
When different categories of
obesity are compared , blood pressure and one or more of the lipids show an increasing trend but
glucose or
insulin level shows no significant change . -
pubmed
Even where a relationship exists , as with
triglyceride and
HDL cholesterol levels , the magnitude of the response is lower among the Inuit . -
pubmed
These advantages are related to high affinity at the
5 - HT2 binding site , no association with an alteration in
dopamine A9 firing rates , and lower
D2 striatal receptor blockade of
olanzapine . -
pubmed
Allene oxide synthase (
AOS ;
hydroperoxide dehydratase ;
EC 4.2.1.92 ) catalyzes the first step in the biosynthesis of
jasmonic acid from
lipoxygenase - derived
hydroperoxides of free
fatty acids . -
pubmed
AOS was induced in leaves by wounding , systemin ,
12 - oxophytodienoic acid , and
methyl jasmonate . -
pubmed
Salicylic acid inhibited
AOS and
PIN II expression , and the addition of
12 - oxophytodienoic acid or
methyl jasmonate did not prevent the inhibition of
PIN II expression in the presence of
salicylic acid . -
pubmed
Ethylene induced the expression of
AOS , but the presence of
ethylene alone did not produce an optimal induction of
PIN II . -
pubmed
The addition of
silver thiosulfate , an
ethylene action inhibitor , prevented the
wound - induced expression of both
AOS and
PIN II . -
pubmed
In the light and in the presence of organic compounds , strain OS -
H1 oxidized
sulfide to elemental
sulfur and
polysulfides , but was not capable of photoautotrophic growth . -
pubmed
Wnt-7a , expressed in the dorsal ectoderm , encodes a key
molecule implicated in these events . -
pubmed
Human chorionic gonadotropin or an implant containing a
gonadotropin - releasing hormone analog was used to initiate follicular and oocyte maturation . -
pubmed
Reprogramming of gene expression in cultured cardiomyocytes and in explanted hearts by the
myosin ATPase inhibitor
butanedione monoxime . -
pubmed
BACKGROUND :
Butanedione monoxime (
BDM ) is a reversible
myosin ATPase inhibitor . -
pubmed
Accumulation of catabolic intermediates was investigated with three classes of
Tn5 mutants with the following
polycyclic aromatic hydrocarbon (
PAH)-negative phenotypes ; ( class I NAP(- ) PHE(- ) FLA(- ) , class
II NAP(- ) PHE(- ) , and class III FLA(- ) ) . -
pubmed
Mutant
200pbhA and other class I mutants lost the ability to catalyze the initial dihydroxylation step and did not transform
NAP ,
ANT ,
PHE , or
FLA . -
pubmed
When mutant
132pbhC was incubated with
1 - hydroxy-2 - naphthoic acid , it accumulated
trans - o - hydroxybenzylidene pyruvate . -
pubmed
Acenaphthenone was oxidized via incorporation of one molecule of
dioxygen by another
oxygenase . -
pubmed
Gonadotropin - releasing hormone (
GnRH)-antagonists can suppress the pituitary hormone secretion completely within a few hours , allowing the avoidance of premature luteinization within controlled
ovarian hyperstimulation ( COH ) for assisted reproductive technologies (
ART ) by midcycle administration . -
pubmed
Two different protocols were described , which were widely used in COH in several phase
II and III studies as well as in clinical practice since the
GnRH - antagonists
Cetrorelix ( Cetrotidesound recording copyright sign ; Serono International S.A. , Geneva , Switzerland ) and Ganirelix ( Orgalutansound recording copyright sign , Antagonsound recording copyright sign ; Organon , Oss , The Netherlands ) are available on the market . -
pubmed
Estradiol secretion is not compromised by the
GnRH - antagonists using recombinant
follicle - stimulating hormone (
FSH ) for COH . -
pubmed
The incidence of a premature leutinizing hormone (
LH ) surge is far below 2 % while the pituitary response remains preserved , allowing the induction of ovulation by
GnRH or
GnRH - agonists . -
pubmed
The two ionized forms of
ascorbate present in aqueous solution reduce
SNP in the order A2->>HA->>H2A (
H2A =
L - ascorbic acid ) to release
NO . -
pubmed
In the frontal cortex , regional metabolic
glucose response to m -
CPP was entered into a group ( impulsive aggressive , control ) x slice ( dorsal , middle , orbital ) x position ( medial , lateral ) x location ( anterior , posterior ) x hemisphere ( right , left ) mixed - factorial analysis of variance design . -
pubmed
While
estrogen has been shown to induce osteoblastic
OPG production , the effects of
androgens on
OPG production have not been defined . -
pubmed
Inhibition of
OPG mRNA levels and protein production by
5alpha - DHT was completely abrogated by the
AR antagonist ,
hydroxyflutamide (
OHF ) , indicating that these effects are directly mediated by the
AR . -
pubmed
Of note ,
OHF alone increased
OPG mRNA levels and protein secretion by 2- to 3 - fold . -
pubmed
Moreover ,
5alpha - DHT and
testosterone also decreased
OPG protein secretion by 40 - 46 % in the untransformed
MSC cells , while
OHF stimulated it . -
pubmed
In conclusion , we demonstrate that
androgens specifically inhibit
OPG mRNA levels and protein secretion by osteoblastic cells . -
pubmed
RESULTS : Most pronounced
BR to
histamine was observed in
allergic asthmatics ( n = 28 ) , irrespective of the presence or absence of
allergic rhinitis . -
pubmed
The time - course accumulation of the
inositol phosphate in response to
GnRH revealed that
bfGnRHR-1 and - 2 were rapidly desensitized , whereas
bfGnRHR-3 was slowly desensitized . -
pubmed
These results , therefore , suggest that differential regulatory mechanisms for desensitization and internalization of the
GnRHR are involved in diverse cellular and physiological responses to
GnRH stimulation . -
pubmed
The
enzyme showed specificity for
adenosine and exhibited Michaelis - Menten Kinetics with kappa(cat ) of 1112.41 min(-1 ) and K(m ) of 14.7 microM at pH 7.5 . -
pubmed
Purine riboside inhibited the
enzyme competitively with K(i ) of 16 microM. -
pubmed
Antidepressants ,
lithium , and
valproate indirectly regulate a number of factors involved in cell survival pathways , including
CREB ,
BDNF ,
Bcl-2 , and
MAP kinases , and may thus bring about some of their delayed long term beneficial effects via underappreciated neurotrophic effects . -
pubmed
Dopamine released during bursts feeds back onto
D ( 2 ) autoreceptors that depress neuronal activity . -
pubmed
New findings from Ingram and colleagues suggest that , by contrast , tonic activity excites these neurons by activating an uncoupled
Cl- conductance that is mediated by the
dopamine uptake transporter . -
pubmed
These 16 genes , nine (
IFN inducible protein 9 - 27 ,
MAA A32 ,
CCLB protein ,
integrin beta4 ,
XRCC1 ,
K8 ,
K18 ,
MT3 ,
MAPKK6 ) of which were altered in a chemical - specific manner and seven (
MIC1 ,
bikunin ,
MTS1 ,
BMP4 ,
RAD23A ,
DOC2 ,
vimentin ) of which were commonly affected by the
MNNG and
As or mixture treatments , were examined for expression in detail by real - time RT - PCR . -
pubmed
Although
OCPs were detected after culturing for a further 5 days ( phase II ) , the reduction of OCPs in phase I was maintained in phase
II . -
pubmed
Lithium interaction with the
cyclooxygenase 2 inhibitors
rofecoxib and
celecoxib and other nonsteroidal anti - inflammatory drugs . -
pubmed
We sought to determine whether NSAIDs that selectively inhibit
cyclooxygenase ( COX ) 2 also elevate serum
lithium concentrations . -
pubmed
METHOD : The U.S. Food and Drug Administration 's Adverse Event Reporting System ( AERS ) database was searched in January 2003 for reports of interactions between
lithium and
rofecoxib or
celecoxib , the selective
COX-2 inhibitors marketed in the United States . -
pubmed
RESULTS : Eighteen cases of increased serum
lithium concentrations after the addition of one of the
COX-2 inhibitors to stable
lithium therapy were retrieved from AERS , 13 with
rofecoxib and 5 with
celecoxib . -
pubmed
Mutants deficient in
glucose-6 - phosphate dehydrogenase and
6 - phosphogluconate dehydrogenase were constructed by disrupting the
zwf and
gnd genes and were grown in minimal media with two different
carbon sources , such as
glucose or
pyruvate . -
pubmed
The measurement of enzyme activities implied a significant change in metabolism , when alternative pathways such as the Entner - Doudoroff pathway ( EDP ) and the malic enzyme pathway were activated in the
gnd mutant grown on
glucose . -
pubmed
As compared with the parent
strain , the activities of
phosphoglucose isomerase were increased in mutants grown on
glucose but decreased in mutants grown on
pyruvate . -
pubmed
AIM : To compare costs of treatment with
risperidone or
olanzapine in a naturalistic setting . -
pubmed
Neutrophil serine proteinases inactivate
surfactant protein D by cleaving within a conserved subregion of the
carbohydrate recognition domain . -
pubmed
The
NSPs generated similar , relatively stable ,
disulfide cross - linked immunoreactive fragments of approximately 35 kDa ( reduced ) , and sequencing of a major catheptic fragment definitively localized the major sites of cleavage to a highly conserved subregion of the
carbohydrate recognition domain . -
pubmed
Chemical double mutant cycles have been used to quantify the interactions of
halogens with the
faces of aromatic rings in
chloroform . -
pubmed
The
halogens are forced over the
face of an aromatic ring by an array of
hydrogen - bonding interactions that lock the complexes in a single , well - defined conformation . -
pubmed
Erythrocyte
glutathione and serum total
glutathione - S - transferase in
epilepsy . -
pubmed
Regionally ,
NAA / Cho was lowered in the internal capsule (
AMN 1.30 + /- 0.20 vs controls 1.69 + /- 0.37 ; p = 0.002 ) and in parieto - occipital white matter (
AMN 1.45 + /- 0.19 vs controls 1.78 + /- 0.55 ; p = 0.04 ) . -
pubmed
NAA /
Cr was lowered in parieto - occipital white matter (
AMN 2.34 + /- 0.31 vs controls 2.83 + /- 0.71 ; p = 0.04 ) . -
pubmed
Serum concentrations of lipids ,
glucose ,
insulin , total
testosterone ( TT ) ,
SHBG ,
estradiol (
E2 ) , and
dehydroepiandrosterone sulfate (
DHEA - S ) were measured . -
pubmed
Bioavailable
testosterone ( BT ) was calculated using TT and
SHBG . -
pubmed
The
metabolic syndrome was defined according to the National
Cholesterol Education Program definition , and
insulin sensitivity was calculated by use of the quantitative
insulin sensitivity check index . -
pubmed
RESULTS : Multiple logistic regression analyses showed an inverse relationship according to 1 sd increase for circulating TT [ odds ratio ( OR ) = 0.43 ; 95 % confidence interval ( CI ) , 0.32 - 0.59 ] , BT ( OR = 0.62 ; 95 % CI , 0.46 - 0.83 ) ,
SHBG ( OR = 0.46 ; 95 % CI , 0.33 - 0.64 ) , and
DHEA - S ( OR = 0.76 ; 95 % CI , 0.56 - 1.02 ) with the
metabolic syndrome . -
pubmed
Linear regression analyses showed that higher TT , BT , and
SHBG levels were related to higher
insulin sensitivity ; beta - coefficients ( 95 % CI ) were 0.011 ( 0.008 - 0.015 ) , 0.005 ( 0.001 - 0.009 ) , and 0.013 ( 0.010 - 0.017 ) , respectively , whereas no effects were found for
DHEA - S and
E2 . -
pubmed
CONCLUSIONS : Higher
testosterone and
SHBG levels in aging males are independently associated with a higher
insulin sensitivity and a reduced risk of the
metabolic syndrome , independent of
insulin levels and body composition measurements , suggesting that these hormones may protect against the development of
metabolic syndrome . -
pubmed
The anticonvulsant potency of the competitive antagonist of the
N - methyl - D - aspartate ( NMDA ) receptor ,
2 - amino-7 - phosphonoheptanoic acid (
APH ) was investigated in the model of
epilepsy induced by systemic administration of
metaphit (
1 -( 1 -( 3 - isothiocyanatophenyl )- cyclohexyl )- piperidine ) . -
pubmed
Somatostatin analogues can be useful in reducing gastric acid hypersecretion ,
serum gastrin and gastric enterochromaffin - like cells , and can thus contribute to treating the disease more effectively . -
pubmed
PBDEs and
BB 153 were quantified using a gas chromatograph coupled with a mass spectrometer (
MS ) operated in electron - capture negative ionization mode . -
pubmed
Intracellular
glutathione (
GSH ) , mitochondrial membrane potential ( MMP ) , cell viability (
propidium iodide uptake ,
PI ) , surface
phosphatidylserine (
Annexin V ) , T - lymphocyte (
CD3 ,
CD4 ,
CD8 ) , and B - lymphocyte (
CD19 ) phenotype distribution and number were assessed . -
pubmed
Regardless of training status , an acute bout of strenuous exercise decreased the total and phenotype specific (
CD3 ,
CD4 ,
CD8 ) number of cells ,
MMP , and
GSH levels immediately after exercise . -
pubmed
Carbachol (
CCh)-induced desensitization to CCh was interrupted by a transient resensitization during its early stage , with concomitant changes at the
muscarinic receptor /
G - protein level in smooth muscle of guinea - pig taenia caeci . -
pubmed
Surface tension , fluorescence , and circular dichroism ( CD ) methods have been used to investigate the interaction between cationic gemini surfactant
1,2 - ethane bis ( dimethyldodecylammonium bromide ) (
C12C2C12 ) and proteins including
bovine serum albumin (
BSA ) and
gelatin . -
pubmed
Addition of
C12C2C12 has a different effect not only on the polarity of the microenvironment in
BSA and gelatin systems but also on their fluorescence spectra . -
pubmed
It can be seen from far - UV CD spectra that the alpha - helical network of
BSA is disrupted and its content decreases from 41.7 % to 27.6 % while the random
coil content of gelatin increases from 53.0 % to 55.9 % with increasing
C12C2C12 concentration . -
pubmed
The results from near - UV CD spectra show that the binding of
C12C2C12 induces changes of the microenvironment around the aromatic
amino acid residues and
disulfide bonds of
BSA at high
C12C2C12 concentrations . -
pubmed
Crystals of the
human immunodeficiency virus 1 ( HIV-1 ) subtype C protease (
PR ) complexed with the clinically used inhibitors
indinavir (
IDV ) and
nelfinavir (
NFV ) have been grown in the monoclinic space group P2(1 ) , with mean unit - cell parameters a = 46.7 ( + /-0.1 ) , b = 59.8 ( + /-0.3 ) , c = 87.0 ( + /-0.4 ) A , beta = 95.2 ( + /-0.5 ) degrees . -
pubmed
The DNA methylation state of 11
CpG sites of
surfactant protein ( SP )- A1 , - B , - C , and - D was determined using universal bead arrays . -
pubmed
Normal lung tissues with a higher level of unmethylated
SP - A1_1468 and
SP - D_1170 CpG exhibited a higher level of
SP - A1 and
SP - D gene transcripts indicating that
CpG methylation may play a role in gene expression . -
pubmed
Both
NAC and
WY14643 , a
peroxisome proliferator - activated receptor ( PPAR )- alpha agonist attenuated these LCM - induced effects in OL - progenitors . -
pubmed
Similar to
WY14643 ,
NAC attenuated LCM - induced inhibition of
PPAR - alpha activity in developing OLs . -
pubmed
Studies conducted with
cytokines and
diamide ( a
thiol - depleting agent ) confirmed that
cytokines are active agents in LCM which may be responsible for inhibition of OL development via
peroxisomal dysfunction and induction of oxidative stress . -
pubmed
Collectively , these data provide evidence that the modulation of
PPAR - alpha activity , thus peroxisomal function by
NAC attenuates LPS - induced glial factors - mediated inhibition of OL development suggesting new therapeutic interventions to prevent the devastating effects of
maternal infections . -
pubmed
Involvement of
lysine 1047 in
type I collagen - mediated activation of polymorphonuclear neutrophils . -
pubmed
post - translational binding of
cyanate to
lysine epsilon -
NH ( 2 ) groups ) impairs PMN oxidative functions , suggesting the potential involvement of
lysine residues in this process . -
pubmed
The present study was devoted to the identification of
lysine residues involved in the
collagen - induced activation of PMNs . -
pubmed
The inhibition of PMN activation by
collagen in the presence of
6 - amino - hexanoic acid , a structural analogue of
lysine residues , confirmed the involvement of specific
lysine residues . -
pubmed
Modification of
lysine residues by carbamylation demonstrated that only one residue , located within the
alpha ( 1 ) CB6 collagen peptide , was involved in this mechanism . -
pubmed
A recombinant alpha(1)CB6 peptide , designed for the substitution of
lysine 1047 by
glycine , exhibited decreased activity , demonstrating that the
lysine residue at position 1047 within the
collagen molecule played a significant role in the mechanism of activation . -
pubmed
These results help to understand in more detail the
collagen - mediated PMN activation mechanism and confirm the prominent involvement of
lysine residues in interactions between extracellular matrix proteins and inflammatory cells . -
pubmed
Help from
CD4 T cells may be required for optimal generation and maintenance of memory
CD8 T cells and also for optimal
Ag reactivation . -
pubmed
We examined whether the helper cell and the
CD8 killer cell need to have the same
Ag specificity for help to be effective during interactions of memory T cells with mature
APC . -
pubmed
Results were as follows for B ( 10(-9 ) K cm(3 ) s(-1 ) ) , E(a ) ( K ) , and k(298 ) ( 10(-12 ) cm(3 ) s(-1 ) ):
cyclohexane , 3.24 + /- 0.14 , 332 + /- 12 , 7.13 ;
cyclo - octane , 3.47 + /- 0.30 , 149 + /- 26 ,
14.1 ;
2 - methylhexane , 1.45 + /- 0.08 , 110 + /- 15 , 6.72 ;
3 - methylhexane , 1.50 + /- 0.08 , 128 + /- 16 , 6.54 ;
methylcyclopentane , 1.65 + /- 0.07 , 109 + /- 13 , 7.65 ;
methylcyclohexane , 1.86 + /- 0.09 , 83 + /- 14 , 9.43 ;
methylcycloheptane , 3.45 + /- 0.45 , 142 + /- 36 , 14.4 ;
n - propylcyclohexane , 2.83 + /- 0.14 , 112 + /- 15 , 13.0 ;
isopropylcyclohexane , 1.79 + /- 0.11 , -44 + /- 34 , 13.9 . -
pubmed
However , the
LTF avoids a distant donor site , provides morphologically similar donor tendon that is readily accessible , and avoids morbidity that may be associated with the use of
DTG . -
pubmed
Effect of
erythromycin A and its new derivative
EM201 on
type I collagen production by cultured dermal fibroblasts . -
pubmed
In this study , we investigated the effects of
erythromycin A (
EMA ) and its new derivative EM201 on
type I collagen production by cultured dermal fibroblasts . -
pubmed
Dermal fibroblasts were cultured with 10(-9 ) M-10(-5 ) M
EMA or EM201 , and
collagen production was measured by incubation with radioactive
proline ,
SDS -
polyacrylamide gel electrophoresis and fluorography . -
pubmed
The results showed that both
EMA and EM201 increased
collagen production and
type I collagen mRNA level ( to a maximum of 200 % with
EMA and 250 % with EM201 ) in a dose - dependent manner in cultured dermal fibroblasts . -
pubmed
Transcription of the
type I collagen gene was also increased by both
macrolides . -
pubmed
Structural basis of
cyanide inhibition of
Ni ,
Fe - containing
carbon monoxide dehydrogenase . -
pubmed
Carbon monoxide dehydrogenases (
CODHs ) catalyze the reversible oxidation of
carbon monoxide with water to
carbon dioxide , two protons , and two electrons . -
pubmed
The
CODHs of anaerobic microorganisms harbor a complex
Ni /
Fe / S - containing metal center called a C - cluster in their active site , which activates the substrates water and
carbon monoxide , stabilizes an intermediary metal -
carboxylate , and transiently stores the two electrons generated in the reaction . -
pubmed
Several small molecules have been reported to inhibit
carbon monoxide oxidation by
CODHs , among which the
cyanide anion acts as a slow binding inhibitor . -
pubmed
Tetrahydroxy stilbene glucoside reduces the
cognitive impairment and overexpression of
amyloid precursor protein induced by
aluminum exposure . -
pubmed
The effect of
TSG was evaluated by passive avoidance task , and
APP expression was evaluated by Western blotting . -
pubmed
Both
TSG and VE significantly ameliorated the performance impairment in the passive avoidance task , and suppressed the over - expression of
APP . -
pubmed
c - Jun activation is required for
4 - hydroxytamoxifen - induced cell
death in
breast cancer cells . -
pubmed
The
c - Jun N - terminal kinase (
JNK ) has been shown to mediate
tamoxifen - induced apoptosis in
breast cancer cells . -
pubmed
However , the downstream mediators of the
JNK pathway linking
tamoxifen to effectors of apoptosis have yet to be identified . -
pubmed
We show that before DNA fragmentation and
caspase 3 / 7 activation , cytotoxic concentrations of
4 - hydroxytamoxifen (
OHT ) induced
JNK - dependent phosphorylation of
c - Jun at
JNK sites earlier shown to regulate
c - Jun - mediated apoptosis . -
pubmed
In addition ,
OHT induced
ERK - dependent expression of
c - Fos and transactivation of an
AP-1 - responsive promoter . -
pubmed
In particular , the ectopic expression of dominant - negative constructs blocking either
AP-1 activity or
c - Jun N - terminal phosphorylation prevented DNA fragmentation after
OHT treatment . -
pubmed
Taken together , our results indicate that the
c - Jun /
c - Fos AP-1 complex has a pro - apoptotic role in
OHT - treated
cancer cells and suggest that pharmacological boosts of
c - Jun activation may be useful in a combination therapy setting to sensitize
cancer cells to
tamoxifen - mediated cell
death . -
pubmed
Regulation of conductance by the number of fixed positive charges in the intracellular vestibule of the
CFTR chloride channel pore . -
pubmed
Rapid
chloride permeation through the
cystic fibrosis transmembrane conductance regulator (
CFTR )
Cl (- ) channel is dependent on the presence of fixed positive charges in the permeation pathway . -
pubmed
Thus , the mutant channel K95S / S1141 K showed
Cl (- ) conductance and open - channel blocker interactions similar to those of wild - type
CFTR , thereby " rescuing " the effects of the charge - neutralizing K95S mutation . -
pubmed
Thus , the
Cl (- ) channel function of
CFTR is maximal with a single fixed positive charge in this part of the inner vestibule of the pore , and increasing the number of such charges to two causes a net decrease in overall
Cl (- ) transport through a combination of failure to increase
Cl (- ) conductance and increased susceptibility to channel block by cytosolic substances . -
pubmed
2 - Amino-3,8 - dimethylimidazo [ 4,5 - f ] quinoxaline (
MeIQx)-induced
hepatocarcinogenesis is not enhanced by
CYP1A inducers ,
alpha - and beta - naphthoflavone : relationship to intralobular distribution of
CYP1A expression . -
pubmed
MeIQx is metabolically activated by
CYP1A2 and then
N - acetyltransferase (
NAT ) , findings that suggest that its carcinogenic potential might be enhanced by simultaneous exposure to chemical(s ) inducing
CYP1A2 . -
pubmed
Unexpectedly , no modifying influence of
naphthoflavones on
MeIQx - induced
hepatocarcinogenesis was demonstrated with reference to
glutathione S - transferase placental form (
GST - P ) positive foci in the liver , although up - regulation of
CYP1A2 was detected on Western blot analysis . -
pubmed
This difference in intralobular distribution of
CYP1A might be related to the fact that
MeIQx hepatocarcinogenesis was not modified by the two
CYP1A inducers . -
pubmed
Charcoal hemoperfusion (
HP ) was performed for 4 hours . -
pubmed
Crizotinib for
EML4 -
ALK positive
lung adenocarcinoma : a hope for the advanced disease ? -
pubmed
Further clinical studies are needed in order to assess if
crizotinib , an
ALK inhibitor , is able to increase the efficacy of the conventional chemotherapy in this disease subset . -
pubmed
Urinary excretion of
3HIA - carnitine was determined by liquid chromatography - tandem
MS on d 0 , 14 , and 28 ( depletion ) and on d 35 and 50 ( repletion ) . -
pubmed
One structure corresponds to a prefusion state , in which
ATL molecules in apposing membranes interact through their
GTPase domains to form a dimer with the
nucleotides bound at the interface . -
pubmed
Here , we show that a
CD44 variant (
CD44v ) interacts with
xCT , a
glutamate -
cystine transporter , and controls the intracellular level of
reduced glutathione (
GSH ) . -
pubmed
Here we used the yeast two - hybrid system to demonstrate that
FGFRL1 binds with its C - terminal ,
histidine - rich domain to
Spred1 and to other proteins of the
Sprouty /
Spred family . -
pubmed
We report that crystal cells express elevated levels of
Sima protein orthologous to mammalian
hypoxia - inducible factor - alpha (
Hif - alpha ) even under conditions of normal
oxygen availability . -
pubmed
ZHER2:477 is a commercially available anti -
HER2 affibody . -
pubmed
One of these 2 - helix proteins is MUT -
DS , which has alpha - helices 1 and 2 bundles , with a
disulfide bridge being formed between the two inserted
homocysteines ( 10 - 12 ) . -
pubmed
The helix conformation of MUT -
DS has been shown to be improved with the placement of a
disulfide bridge . -
pubmed
To explore cell - autonomous functional differences between naive and memory
CD8 T cells that are not influenced by differences in the priming environment , an experimental model has been developed in which physiological numbers of both populations of cells were cotransferred into naive hosts before
Ag stimulation . -
pubmed
Modulation of
FoxO1 phosphorylation / acetylation by
baicalin during aging . -
pubmed
Included is its ability to suppress reactive species ( RS ) producing activity and modulate
nuclear factor - kappaB through cellular redox regulation with enhanced
thiol ability . -
pubmed
In the present study , we attempted to elucidate the molecular modulation of antioxidant
baicalin on the
insulin - induced
FoxO1 inactivation . -
pubmed
Our results further reveal that
baicalin regulated
FoxO1 phosphorylation via
PI3K /
Akt by
insulin and
FoxO1 acetylation by the interaction of
CBP and
SIRT1 , leading to changes in
catalase gene expression during aging . -
pubmed
TLR2 /
MyD88 /
NF - kappaB pathway , reactive
oxygen species ,
potassium efflux activates
NLRP3 /
ASC inflammasome during
respiratory syncytial virus infection . -
pubmed
Here , we compare the
EGFR binding affinities of GE11 ,
EGF , and their
polyethyleneimine -
polyethyleneglycol (
PEI -
PEG ) conjugates . -
pubmed
We found that although GE11 by itself does not exhibit measurable affinity to the
EGFR , tethering it to
PEI -
PEG increases its affinity markedly , and complex formation with
polyinosine /
cytosine ( polyIC ) further enhances the affinity to the submicromolar range . -
pubmed
PolyIC / PPGE11 has a similar strong antitumor effect against
EGFR overexpressing
tumors in vitro and in vivo , as polyIC /
polyethyleneimine -
polyetheleneglycol -
EGF ( polyIC /
PP - EGF ) . -
pubmed
BACKGROUND : Intravenous (
IV )
artesunate is the treatment of choice for severe
malaria . -
pubmed
CONCLUSIONS : Data from the named patient programme demonstrate that
IV artesunate is effective and well - tolerated in European travellers lacking immunity . -
pubmed
Treatment of
IV artesunate should be limited to the period that
IV treatment is required and should be followed by a full oral course of an appropriate anti - malarial drug . -
pubmed
Zinc - binding domain of the
bacteriophage T7 DNA primase modulates binding to the DNA template . -
pubmed
The
zinc - binding domain ( ZBD ) of prokaryotic
DNA primases has been postulated to be crucial for recognition of specific sequences in the single - stranded DNA template . -
pubmed
The ability of
T7 DNA primase to catalyze template - directed
oligoribonucleotide synthesis is eliminated by substitution of any five -
amino acid residue - long segment within the ZBD . -
pubmed
The role of this region in
primase function was further investigated by generating a protein library composed of multiple
amino acid substitutions for
Pro-16 ,
Asp-18 , and
Asn-19 followed by genetic screening for functional proteins . -
pubmed
The results suggest that the
zinc - binding mode alone is not responsible for sequence recognition , but rather its interaction with the
RNA polymerase domain is critical for DNA binding and for sequence recognition . -
pubmed
CONCLUSION : Of the compounds studied , only
vitamin E and
NAC significantly mitigated the effects of oxidative stress on
RPE cells . -
pubmed
A - type lamins have been shown to interact and sequester activated
phospho -
ERK1 / 2(
pERK1 / 2 ) at the nucleus . -
pubmed
In an assay of gap junctional function ,
Lmna(-/- ) MEFs transferred
dye to 60 % fewer partners compared to
Lmna(+/+ ) controls . -
pubmed
TGF - beta1 enhanced
glioma - induced angiogenesis , which was inhibited by
JNK inhibitor
SP600125 but not
p38 MAPK inhibitor
SB202190 ,
ERK inhibitor
PD98059 , or
PI3 K inhibitor
LY294002 , indicating the important role of
TGF - beta1 and
JNK pathways in this process . -
pubmed
Up to the present ,
lepiridin , recently suspended , and
argatroban ( direct
thrombin inhibitors ) have been the approved medicines normally used in treatment , administered in parenteral form . -
pubmed
Dabigatran , a new anticoagulant medicine that is a direct and reversible
thrombin inhibitor , could theoretically be a medicine employed in treating
HIT . -
pubmed
Our work provides a facile and versatile approach for the design and fabrication of smart intracellular targeted nanovehicles for effective
cancer treatment , and opens a new
era in the development of biodegradable
polyurethanes for next - generation nanodelivery systems . -
pubmed
The values of pH ,
TN , WSC , and the content of
NH : - N of composting were 4.58 % , 12.62 % , 49.06 % , and 35.30 % higher than those of the control . -
pubmed
Here we report that
IKBKE regulates
FOXO3a through phosphorylation of
FOXO3a -
Ser644 . -
pubmed
Previous studies have shown that
IKBKE directly activates
Akt and that
Akt inhibits
FOXO3a by phosphorylation of
Ser32 ,
Ser253 and
Ser315 . -
pubmed
, converting 3
serine residues to
alanine ) was inhibited by
IKBKE . -
pubmed
These findings suggest that
IKBKE regulates
FOXO3a primarily through phosphorylation of
SerS644 and that
IKBKE exerts its cellular function , at least to some extent , through regulation of
FOXO3a . -
pubmed
When the inserted
amino acid sequences were aligned with the
Cap protein sequence , eight core regions in
Cap ( ( 50)SRTFGYT(56 ) , ( 62)VRTPSW(67 ) , ( 68)AVDMMR(73 ) , ( 79)FLPPGG(84 ) , ( 86)SNPRSVPF(93 ) , ( 102)KVEFWP(107 ) , ( 119)GSSXXXLDDN(128 ) and ( 229)PPLNP(233 ) ) were identified , three of which ( ( 50)SRTFGYT(56 ) , ( 86)SNPRSVPF(93 ) and ( 102)KVEFWP(107 ) ) for the first time . -
pubmed
Therefore , it was surmised that failure of
irinotecan may be the result of overexpression of the DNA repair enzyme
tyrosyl - DNA phosphodiesterase (
TDP1 ) , which processes
topoisomerase I - DNA complexes resulting from
topoisomerase I inhibitor treatment . -
pubmed
Critically , knockdown of
TDP1 or inhibition of
poly ( ADP - ribose ) polymerase-1 (
PARP-1 ) , an enzyme in the same complex as
TDP1 , sensitized
rhabdomyosarcoma cell lines to analogues of
irinotecan . -
pubmed
BACKGROUND : The Acute Study of Clinical Effectiveness of
Nesiritide in Decompensated
Heart Failure ( ASCEND -
HF ) randomly assigned 7,141 participants to
nesiritide or placebo . -
pubmed
Recent work implicates a lipid critical for neuronal function ,
phosphatidylinositol-4,5 - bisphosphate [
PI ( 4,5 ) P2 ] , in
Abeta - induced
synaptic and behavioral impairments . -
pubmed
Synaptojanin 1 (
Synj1 ) , a lipid phosphatase mediating the breakdown of
PI ( 4,5 ) P2 , has been shown to play a role in synaptic vesicle recycling and receptor trafficking in neurons . -
pubmed
To this end , we developed a screening assay for
Synj1 based on detection of inorganic
phosphate liberation from a water - soluble , short - chain
PI ( 4,5 ) P2 . -
pubmed
The assay displayed saturable kinetics and detected
Synj1 's substrate preference for
PI ( 4,5 ) P2 over
PI ( 3,4,5 ) P3 . -
pubmed
Sunitinib is a multiple
tyrosine kinase receptor inhibitor that is approved for the treatment of
metastatic renal cell carcinoma (
RCC ) . -
pubmed
Samples of the intestinal tissue were used for histological evaluation and assays for
glutathione (
GSH ) levels ,
malonyldialdehyde (
MDA ) concentration ,
myeloperoxidase (
MPO ) activity ,
nitrate and
nitrite (
NO3 /
NO2 ) concentration and
cytokines levels . -
pubmed
Additionally , it avoided the consumption of
GSH , decreased pro - inflammatory
cytokine levels ,
MDA and
NO3 /
NO2 concentrations and diminished the
MPO activity . -
pubmed
CONCLUSIONS : Our results suggest that the
PLS fraction has a protective effect against
intestinal damage through mechanisms that involve the inhibition of inflammatory cell infiltration ,
cytokine releasing and lipid peroxidation . -
pubmed
Prostacyclin regulates bone growth via the
Epac /
Rap1 pathway . -
pubmed
In growth plate chondrocytes , inhibition of proliferation and
ERK activation by NSAIDs is reversed by
forskolin ,
8 - bromoadenosine ,
3 ' , 5 '- cAMP and a
prostacyclin analog ,
iloprost . -
pubmed
The inhibition of proliferation and
ERK activation by
celecoxib is also reversed by
8CPT-2Me - cAMP , an activator of
Epac , implicating the
small G protein Rap1 in the pathway activated by
iloprost . -
pubmed
Bloods showed
potassium of 1.8 mEq / L ( normal range 3.5 - 5 ) , free T4 of 29.2 pmol / L ( normal range 6.5 - 17 ) and
thyroid - stimulating hormone (
TSH ) of & lt;0.01 mIU / L ( normal range 0.35 - 4.94 ) . -
pubmed
The first step in
caffeine metabolism is mediated for over 95 % by the
CYP1A2 isoform of
cytochrome P450 . -
pubmed
Therefore ,
CYP1A2 activity is most conveniently measured through the determination of
caffeine clearance . -
pubmed
, G-259 - T and C-248 - T ) in
gyrA leading to
Asp-87 -
Tyr and
Ser-83 -
Phe substitutions in
GyrA , respectively , were detected . -
pubmed
Of all plasmid - mediated
quinolone resistance genes tested , only
qnrS ( 4.1 % ) and
qnrB ( 1.8 % ) were found . -
pubmed
ii ) The absence of exATP generation by the enzyme , and the finding that exATP hydrolysis was largely
oligomycin - insensitive , are in line in parental cells with the deficit of
OSCP and suggest the occurrence of sub - assemblies together evoking more regulation by
IF1 . -
pubmed
Mitochondrial Lon protease (
Lon ) regulates several mitochondrial functions , and is inhibited by the anticancer molecule
triterpenoid 2 - cyano-3 , 12 - dioxooleana-1,9 ( 11 )- dien-28 - oic acid (
CDDO ) , or by its
C-28 methyl ester derivative (
CDDO - Me ) . -
pubmed
In particular , they : i ) cause depolarization ; ii ) increase mitochondrial ROS , iii ) alter mitochondrial morphology and proteins involved in mitochondrial dynamics ; iv ) affect the levels of
Lon and those of
aconitase and
human transcription factor A , which are targets of
Lon activity ; v ) increase level of protein
carbonyls in mitochondria ; vi ) lead to intrinsic apoptosis . -
pubmed
Concomitantly ,
farnesol dose - dependently increased
glucose uptake and
glucose transport protein 4 (
GLUT4 ) expression without affecting cell viability . -
pubmed
Furthermore , quantitative real - time
polymerase chain reaction and Western blot showed that
farnesol increased the mRNA and protein levels of
peroxisome proliferator - activated receptor gamma (
PPARgamma ) , a key regulator of adipocyte differentiation , and the mRNA levels of
PPARgamma - regulated
fatty acid - binding protein 4 and
adiponectin ; in contrast ,
farnesol downregulated
Pref-1 gene , a marker of pre - adipocytes . -
pubmed
GW9662 ( 10 micromol / L ) , an antagonist of
PPARgamma , reversed the effects of
farnesol on cellular lipid content , suggesting that
PPARgamma signaling pathway may mediate the
farnesol effect . -
pubmed
Farnesol ( 25 - 75 mumol / L ) did not affect the mRNA level of
3 - hydroxy-3 - methylglutaryl coenzyme A reductase , the rate - limiting enzyme in the
mevalonate pathway . -
pubmed
Farnesol may be the
mevalonate - derived inducer of adipocyte differentiation and potentially an
insulin sensitizer via activation of
PPARgamma and upregulation of
glucose uptake . -
pubmed
Hormones , such as
estrogen ,
follicle stimulating hormone , and
leptin , transcription factors , such as
Runx2 and
osterix , and the
wnt signaling pathway are discussed in terms of their roles in bone cell differentiation and function . -
pubmed
Glutamate treatment for 30 min induced the permeabilization of lysosomal membranes as assessed by
acridine orange redistribution and immunofluorescence of
cathepsin B in the cytoplasm . -
pubmed
Inhibition of
glutamate excitotoxicity by the
NMDA receptor antagonist
MK-801 and the
calcium chelator
ethylene glycol - bis ( 2 - aminoethylether )- N , N , N ' , N '- tetraacetic acid , rescued lysosomes from permeabilization . -
pubmed
The role of calpain and reactive
oxygen species ( ROS ) in inducing
LMP was also investigated . -
pubmed
It has been reported that
lysosomal - associated membrane protein 2 (
LAMP2 ) and
heat shock protein ( HSP ) 70 are two calpain substrates that promote
LMP in
cancer cells ; however , it was found that calpains were activated by
glutamate , but only
LAMP2 was subsequently degraded . -
pubmed
Furthermore ,
LMP was not alleviated by treatment with the
calpain inhibitors calpeptin and
SJA6017 , which blocked the cleavage of the calpain substrate
alpha - fodrin . -
pubmed
It was demonstrated that
LMP was significantly alleviated by treatment with the antioxidant
N - Acetyl - L - cysteine , indicating that
LMP involvement in early
glutamate excitotoxicity may be mediated partly by ROS rather than calpain activation . -
pubmed
Overall , these data shed light on the role of ROS - mediated
LMP in early
glutamate excitotoxicity . -
pubmed
The
tumour protein D52 isoform 1 (
PC-1 ) , a member of the
tumour protein D52 (
TPD52 ) protein family , is
androgen - regulated and prostate - specific expressed . -
pubmed
Calcium was estimated in the
reagents used for native gel electrophoresis of
Neuronal Calcium Sensor ( NCS ) proteins . -
pubmed
NCS proteins exhibit
calcium - dependent mobility shift in native gels . -
pubmed
Methylation status of two
CpG islands ( CpG1 and
CpG2 ) in promoter region of
DJ-1 was explored by
bisulfite specific PCR - based sequencing method . -
pubmed
RESULTS :
CpG sites in these two
CpG islands ( CpG1 and
CpG2 ) of
DJ-1 were unmethylated in both
PD and
NC group . -
pubmed
When comparing first and third admission - day laboratory values , the increase in leukocyte counts and the decrease in
CPK ,
urea ,
creatinine , aPTT , PT , INR , and
hemoglobin levels were significant in nonfatal cases . -
pubmed
Extended Spectrum
Beta - Lactamases (
ESBL ) provide high level resistance to
beta - lactam antibiotics among bacteria . -
pubmed
Metformin elicits its therapeutic effects mainly via activation of
AMP - activated kinase (
AMPK ) pathway . -
pubmed
We measured concentrations of
follicle - stimulating hormone (
FSH ) ,
luteinizing hormone (
LH ) , and total
testosterone ( T ) from 234 of their sons at 12 years . -
pubmed
A 10 - fold increase in total prenatal
SigmaPCBs was associated with a 64.5 % increase ( 95 % CI : 8.6 , 149.0 ) in
FSH , primarily driven by non-
dioxin - like congeners . -
pubmed
Furthermore , we observed a crosstalk between the
Nrf2 /
HO-1 signaling pathway and the activation of the
nuclear factor - kappaB (
NF - kappaB )
transcription factor , since administration of
ZnPP IX ( specific inhibitor of
HO-1 ) or
Nrf2 knockdown using small interfering RNA ( siRNA ) abolished the anti - inflammatory effects induced by CA . -
pubmed
Hydrochlorothiazide (
HCTZ ) is a class
IV drug according to the Biopharmaceutical Classification System . -
pubmed
The optimized formulations , with reduced amount of surfactant , and composed of medium chain
triglycerides , Cremophor EL and Transcutol
P did not affect the pH or show drug incompatibilities . -
pubmed
During the induction of
NMDA - receptor - dependent LTP ,
Ca2 + influx stimulates recruitment of synaptic
AMPA (
alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazole propionic acid ) receptors , thereby strengthening synapses . -
pubmed
LTP was restored by expression of wild - type
Syt7 but not of a
Ca2 + -binding - deficient mutant
Syt7 . -
pubmed
Blocking postsynaptic expression of
Syt1 and
Syt7 did not impair basal synaptic transmission , reduce levels of synaptic or extrasynaptic
AMPA receptors , or alter other
AMPA receptor trafficking events . -
pubmed
Moreover , expression of dominant - negative mutant
Syt1 which inhibits
Ca2 + -dependent presynaptic vesicle exocytosis , also blocked
Ca2 + -dependent postsynaptic
AMPA receptor exocytosis , thereby abolishing LTP . -
pubmed
Our results suggest that postsynaptic
Syt1 and
Syt7 act as redundant
Ca2 + -sensors for
Ca2 + -dependent exocytosis of
AMPA receptors during LTP , and thereby delineate a simple mechanism for the recruitment of
AMPA receptors that mediates LTP . -
pubmed
The RUN domain
Beclin-1 - interacting
cysteine - rich - containing (
Rubicon ) protein is involved in the maturation step of autophagy and the endocytic pathway as a
Beclin-1 - binding partner , but little is known regarding the role of
Rubicon during
viral infection . -
pubmed
This study reports the successful structure - based design and synthesis of new
7 - azaindenoisoquinolines that act as triple inhibitors of
Top1 ,
Tdp1 , and
Tdp2 . -
pubmed
Sigma-1 - mediated antihyperalgesia was reversed by the opioid antagonists
naloxone and
naloxone methiodide ( a peripherally restricted
naloxone analog ) and by local administration at the inflamed site of
monoclonal antibody 3 - E7 , which recognizes the pan - opioid sequence
Tyr - Gly - Gly - Phe at the N terminus of most endogenous opioid peptides ( EOPs ) . -
pubmed
Prdx-1 is an anti - apoptotic and stress response protein which protects cells from damage by ROS and
H2O2 . -
pubmed
Inhibition of the
p38 MAPK by a specific inhibitor ,
SB203580 , abrogates the
ESAT-6 mediated induction of
Prdx-1 expression as well as the phosphorylation of
NRF-2 in a time - dependent manner . -
pubmed
The ectoderm had two morphologically and immunocytochemically distinct populations of neurons , the motor nerve net ( MNN ) , which was immunoreactive to the
taurine - like molecule , and the diffuse nerve net ( DNN ) , which was immunoreactive to the neuropeptide
Phe - Met - Arg - Phe - NH2 (
FMRFamide ) . -
pubmed
In conclusion , these results suggest that Nano -
C3 G can reduce UVB - induced
epidermal damage through the
p53 - mediated apoptosis signaling pathway . -
pubmed
Nano - encapsulation of
epigallocatechin gallate in the
ferritin - chitosan double shells : Simulated digestion and absorption evaluation . -
pubmed
EGCG - loaded H-2F complex (
EHF ) was firstly prepared by taking advantages of the reversible self - assembly of the
ferritin , and the
EHF -
CS composite ( EHFC ) was fabricated by electrostatic interactions with binding number n of ( 4.1 + - 0.11 ) and binding constant K of ( ( 5.3 + - 0.2 ) x 105 M-1 ) , respectively . -
pubmed
It was calculated that about 12.6 of
EGCG molecules can be encapsulated in one H-2F
ferritin cage with an encapsulation efficiency of 9.69 % ( w / w ) . -
pubmed
SDS - PAGE analysis indicated that the
CS binding to H-2F could inhibit
ferritin degradation by
pepsin and
trypsin ; the stability of
EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract . -
pubmed
Atomic scale reversible opto - structural switching of few atom luminescent
silver clusters confined in
LTA zeolites . -
pubmed
Luminescent
silver clusters ( AgCLs ) stabilized inside partially
Ag exchanged
Na LTA zeolites show a remarkable reversible on - off switching of their green - yellowish luminescence that is easily tuned by a hydration and
dehydration cycle , making them very promising materials for sensing applications . -
pubmed
lutzii 's
gp43 was not observed in IB or
silver / PAS staining . -
pubmed
Identification of a functional
FADS1 3'UTR variant associated with erythrocyte
n-6 polyunsaturated fatty acids levels . -
pubmed
BACKGROUND : Blood
polyunsaturated fatty acid (
PUFA ) levels are determined by diet and by endogenous synthesis via
Delta5 - and Delta6 - desaturases ( encoded by the
FADS1 and
FADS2 genes , respectively ) . -
pubmed
Genome - wide association studies have reported associations between
FADS1 -
FADS2 polymorphisms and the plasma concentrations of
PUFAs , HDL- and LDL -
cholesterol , and
triglycerides . -
pubmed
METHODS : To address this question , we ( 1 ) genotyped individuals ( n = 540 ) for the rs174547 polymorphism to confirm associations with
PUFA levels used as surrogate estimates of
desaturase activities and ( 2 ) examined the functionality of variants in linkage disequilibrium with rs174547 using bioinformatics and luciferase reporter assays . -
pubmed
RESULTS : The rs174547 minor allele was associated with higher erythrocyte levels of
dihomo - gamma - linolenic acid and lower levels of
arachidonic acid , suggesting a lower
Delta5 - desaturase activity . -
pubmed
In a second study , we performed a retrospective analysis of patients who underwent transesophageal echocardiography ( TEE ) for various reasons , assigned these to 2 groups based on the heart - rhythm (
sinus - rhythm [
SR ] vs
AF ) and examined associations between clinical and echocardiographic parameters and intra - cardiac thrombogenic material . -
pubmed
Norepinephrine was modestly increased while the ratio between plasma
dihydroxyphenylglycol (
DHPG ) and
norepinephrine levels , a marker for
norepinephrine clearance , was reduced with UFP +
O3 . -
pubmed
Both
oxazolidinones caused a concentration- and time - dependent inhibition of
CYTox I expression ,
cytochrome c - oxidase activity and mitochondrial spare capacity . -
pubmed
While the expression of
CYTox I was completely recovered after 6 days of drug washout , we observed only a partial (
linezolid ) or no (
tedizolid ) recovery of
cytochrome c - oxidase activity , and no rescue of mitochondrial spare capacity ( after 3 days ) . -
pubmed
RESULTS : baseline
troponin was 3.00 ( 0.06 ; 5.20 ) ng / mL in the
tirofiban group versus 4.6 ( 0.02 ; 13.10 ) ng / mL in the no -
tirofiban group ( p=0.229 ) , and
post - procedural peak 3.5 ( 0.06 ; 5.50 ) ng / mL versus 6.30 ( 0.09 ; 28.40 ) ng / mL ( p=0.191 ) . -
pubmed
Peak - baseline difference in
troponin was lower in the
tirofiban group than in the no -
tirofiban group : 0.3 ( 0.00 ; 1.7 ) ng / mL versus 1.3 ( 0.01 ; 10.00 ) ng / mL ( p=0.044 ) ; the relative peak - baseline change in
troponin was analogously different : 24.3 % ( 0 % ; 44.7 % ) versus 50 % ( 21.3 % ; 80.0 % ) ( p=0.039 ) . -
pubmed
CONCLUSIONS :
Tirofiban during
CAS might provide peri - procedural myocardial protection and reduce
myocardial injury as determined by serial
troponin measurements . -
pubmed
Herein , a uniform wafer - scale 1T -
WS2 film is prepared using a plasma - enhanced chemical vapor deposition (
PE - CVD ) system . -
pubmed
All the
Pt ( II ) complexes exhibit admixed 3(LC / MLCT ) characters in
T1 states with various proportions , which are strongly structure - dependent . -
pubmed
Compared with wild - type , both VX and
VP transgenic lines grew better at 200mM
NaCl , and also exhibited higher tolerance and faster recovery from water - deficit stress : these performances were associated with more
Na + , K+ and
Ca2 + accumulation in their leaves and roots , which caused lower leaf solute potential and thus retained more water . -
pubmed
FSH ,
LH ,
testosterone , semen volume , age ,
BMI , ethnicity and testicular size on clinical evaluation were included in these models . -
pubmed
The values of affinity energy obtained for the
hydroxychloroquine ligands was -9.9 kcal / mol and for the
chloroquine of -10.8 kcal / mol which indicate that the coupling contributes to an effective improvement of the affinity energies with the
protease . -
pubmed
Alkaline Phosphatase Activity Is a Key Determinant of Vascular Responsiveness to
Norepinephrine . -
pubmed
Here , we tested the hypothesis that
TNAP (
tissue nonspecific alkaline phosphatase ) modulates vascular responsiveness to
norepinephrine . -
pubmed
At 100 micromol / L ,
L - p - BT further reduced mesenteric
TNAP activity and caused an additional significant right - shift of the
norepinephrine concentration versus vasoconstriction relationship . -
pubmed
A higher concentration ( 200 micromol / L ) of
L - p - BT had no further effect on either mesenteric
TNAP activity or
norepinephrine - induced vasoconstriction . -
pubmed
Additional experiments showed that the effects of
TNAP inhibition on
norepinephrine - induced vasoconstriction were not mediated by accumulation of
pyrophosphate or
ATP (
TNAP substrates ) nor by reduced
adenosine levels (
TNAP product ) . -
pubmed
TNAP inhibition significantly reduced the Hillslope of the
norepinephrine concentration versus vasoconstriction relationship from 1.8 + -0.2 ( consistent with positive cooperativity of
alpha1 - adrenoceptor signaling ) to 1.0 + -0.1 ( no cooperativity ) . -
pubmed
Selective activation of
A1 - adenosine receptors , which are known to participate in coincident signaling with
alpha1 - adrenoceptors , reversed the suppressive effects of
L - p - BT on
norepinephrine - induced vasoconstriction . -
pubmed
TNAP modulates vascular responses to
norepinephrine likely by affecting positive cooperativity of
alpha1 - adrenoceptor signaling via a mechanism involving
A1 receptor signaling . -
pubmed
Immobilization of
aminoacylase by adsorption to
tannin immobilized on aminohexyl cellulose . -
pubmed
The immobilization of
aminoacylase (
N - acylamino acid amidohydrolase ,
EC 3.5.1.14 ) was investigated by using
tannin immobilized on aminohexyl cellulose . -
pubmed
The most active immobilized
aminoacylase was obtained when
aminoacylase was adsorbed to the immobilized
tannin in a weak alkaline medium containing
sodium chloride and
n - butanol at 37 degrees C. The activity of the immobilized
tannin - aminoacylase complex per unit volume was five times higher than that of the
DEAE - Sephadex - aminoacylase complex used for industrial production of
L - amino acids in our plants . -
pubmed
In this work a steady - state kinetic analysis has been carried out on the catabolic
ornithine carbamoyltransferase at pH 6.8 in the presence of the allosteric activator ,
phosphate . -
pubmed
coli
enzyme in the reverse direction , the initial velocity patterns converging on the abscissa were obtained with either
citrulline or
arsenate as variable substrate . -
pubmed
Properties of mini - plasmids of the
antibiotic resistance factor R1 carrying deleted and enlarged
ampicillin transposons ( Tn3 ) . -
pubmed
These results suggest that
sigma receptors play a crucial role in the induction of
methamphetamine - induced sensitization . -
pubmed
Of more practical importance , the combined absence of
CK ,
EMA , and
vimentin in
formalin - fixed , paraffin - embedded CCTL virtually precludes
confusion with
renal cell carcinoma . -
pubmed
Uptake by
ER - rich target tissue remained high when
charcoal was used to prevent reabsorption of radiolabel from the digestive tract after its biliary excretion , and when the products of biliary excretion were removed by catheterization of the common bile duct . -
pubmed
Sulfobromophthalein ,
bromophenol blue and
rose bengal , which yielded an in vivo ' counter - transport ' phenomena , markedly inhibited
ANS binding to cytosolic
proteins . -
pubmed
Sulfobromophthalein , which is one of the organic anions exhibiting the in vivo ' counter - transport ' phenomenon , remarkably inhibited
ANS binding to ligandin fraction . -
pubmed
The main representatives of substances with a vasodilatating action are the endothelial relaxation factor and
prostacycline , as to vasoconstrictor substances it is
endothelin ,
thromboxan A2 and some growth factors . -
pubmed
Because of a history of prolonged
infertility , conservative treatment ( including bed
rest , antibiotics and intravaginal
povidone - iodine pessaries ) was given . -
pubmed
From comparison with structural data of other
blue - copper proteins , it is concluded that the
copper coordination environment is relatively rigid , which may be a clue to its high redox potential . -
pubmed
Yeast tRNAPhe was incubated with the enzyme in the presence of double stranded RNA ( in this case polyI - polyC ) and
ATP or
deoxyATP . -
pubmed
The reaction products were analyzed by
ribonuclease T1 digestion of the
tRNA ,
polyacrylamide gel electrophoresis and autoradiography . -
pubmed
Using
ATP , the
2 - 5A synthetase adds one , two or three
AMP residues to the 3'-end of the
tRNA whereas when
dATP is replacing
ATP , only one
nucleotide unit is added . -
pubmed
Within 5 minutes of intravenous injection of antigen , an acute , transient
arthritis occurred in the test joints only , with swelling and extravasation of intravascular blue dye and 125I - labeled
albumin , decreased numbers of stainable mast cells , and decreased
histamine content of the joint synovium . -
pubmed
Pretreatment of experimental animals with
H1 and
H2 antihistamines did not completely block the reaction . -
pubmed
The PMNL - induced vascular smooth muscle relaxation was abolished by both
hemoglobin and
methylene blue and potentiated by both
superoxide dismutase and
captopril . -
pubmed
Although suspension of PMNLs caused release of
eicosanoids ,
thromboxane A2 and
prostacyclin , from rings with intact endothelium , neither
indomethacin nor the
TxA2 - endoperoxide receptor antagonist
SQ 29548 modified the effects of PMNLs on vascular smooth muscle tone . -
pubmed
A sensitive and specific enzyme - linked immunosorbent assay ( ELISA ) for
cholecystokinin octapeptide sulfate (
CCK-8S ) has been developed using N - terminal specific antibody for
CCK-8S. -
pubmed
The latter action suggests an effect on ovarian
aromatase , on testicular
17 alpha / 17,20 - lyase activities and finally on
cholesterol side - chain cleavage . -
pubmed
The increase of
progestins suggests that the
17 alpha / 17,20 - lyase activities are the most sensitive testicular enzymatic reactions to
R 8110 . -
pubmed
Unlike the neurite - promoting and neuronal - survival modulating agent
laminin , it can not be depleted on
poly ( L - alpha - ornithine )-coated plastic surfaces . -
pubmed
NB IMR 32 cell extracts were electrophoresed using
NaDodSO4 / PAGE and transferred to
nitrocellulose . -
pubmed
In contrast to the tolerance that is rapidly observed after repeated administration of LPS ,
MDP does not lose its capacity of inducing serum
CSA after repeated injections . -
pubmed
Repeated daily injections of
MDP also fail to induce tolerance to the LPS -
CSA inducing effect . -
pubmed
This applies to
glycosphingolipids ( GSLs ) and
glycoproteins (
GPs ) . -
pubmed
An important distinction between the two types of glycoconjugates seems to be that
GPs , apart from carrying
carbohydrates , have some biological function which is most likely associated with their protein moieties . -
pubmed
These data suggest that the inhibitory action and potency of
nucleoside dialdehydes on
neuroblastoma growth are independent of their capacity to undergo a
kinase - mediated phosphorylation at the 5 ' position . -
pubmed
Human immunoglobulin M was reduced with concentrations of
2 - mercaptoethylamine chosen so that approximately 40 % of the
immunoglobulin M was reduced to
7S subunits . -
pubmed
Under these conditions , which selectively cleave intersubunit
disulfides ,
J chain was released . -
pubmed
The
high - molecular - weight immunoglobulin M remaining after treatment with
2 - mercaptoethylamine had a sedimentation coefficient of 18.0 S and molecular weight of 1 million , and dissociated in 4 M
guanidine into subunits similar in size to the
7S subunits . -
pubmed
J chain was found in the 18.0S , noncovalently linked
immunoglobulin M from which it was released only after more complete reduction with 10 mM
dithiothreitol . -
pubmed
The nature of chemically or photochemically reduced
flavin of
salicylate hydroxylase . -
pubmed
The guinea - pig ileum was contracted much more slowly than by synthetic LT
C4 or
D4 and this contraction was not antagonized by
FPL 55712 . -
pubmed
In liver
PE ,
PC ,
PS and
PI it was respectively 4.3 , 4.9 , 3.8 and 0.4 , while in the analogous platelet lipids it was 3.0 , 4.0 , 0.9 and 0.6 . -
pubmed
Feeding
linolenate did not markedly elevate the levels of 22:5(n-3 ) or 22:6(n-3 ) in platelet
PI , but the combined amounts of 22:5(n-3 ) and 22:6(n-3 ) in liver
PI were 21.2 % , versus 2.9 % in chow - fed controls . -
pubmed
Again , the 20:5(n-3)/20:4(n-6 ) ratio was lowest for platelet
PI and
PS and liver
PI . -
pubmed
These incorporation studies are consistent with the feeding studies which show that
oleate is converted to 20:3(n-9 ) and incorporated into
PI more readily than the analogous metabolism of 18:3(n-3 ) to 20:5(n-3 ) and its acylation into
PI , which is an important source of
unsaturated fatty acids for
prostaglandin biosynthesis . -
pubmed
A series of
alkyldimethylbenzylammonium chlorides have been synthesized with
n - alkyl chain lengths of
C1 leads to
C18 . -
pubmed
The data fit to quadratic functions estimated for low (
C1 -
C7 ) and high
( C8 - C16 ) alkyl chain length compounds was better than for a single quadratic describing the activity of the complete series (
C1 -
C18 ) . -
pubmed
These maximized at log P values corresponding to
alkyl - chain lengths of approximately
C7 and C14 respectively , and were suggestive of low and high affinity binding sites upon the cell surface . -
pubmed
Evidence for essential
lysines in
heparin cofactor II . -
pubmed
Covalent modification with
pyridoxal 5 '- phosphate was used to study the function of
lysyl residues in
heparin cofactor II , a
heparin - dependent plasma protease inhibitor . -
pubmed
Reduction of the
Schiff base with
sodium borohydride resulted in modification of 3 - 4
lysyl residues of
heparin cofactor II at high concentrations of
pyridoxal 5 '- phosphate , one of which was protected in the presence of heparin . -
pubmed
In contrast to native
heparin cofactor II , the modified
protease inhibitor did not bind to a heparin -
agarose column . -
pubmed
The results suggest that
lysyl residues are essential for heparin cofactor activity during
thrombin inhibition . -
pubmed
[ Comparative study of the quantitative determination of
kanamycin sulfate in ophthalmic films with a
collagen base by microbiological and chemical methods ] . -
pubmed
The results of the comparative study on microbiological and chemical quantitative determination of
kanamycin sulfate in the ophthalmic films with the
collagen base are presented . -
pubmed
The intraocular films prepared with the use of 1 per cent
collagen solution contain
dexamethasone and
kanamycin . -
pubmed
Substitution of
amino acids for
casein was followed by a fall in urinary and plasma
A1 . -
pubmed
In the repair test , larval stock , consisting of meiotic recombination - deficient double mutant
mei-9a mei-41D5 males and repair - proficient females , was exposed to the test
agents , and the preferential killing of the mutant larvae was taken as evidence of the DNA - damaging effect . -
pubmed
RNA and protein analyses indicated that the expression of
p84NF - kappa B1 and
p98NF - kappa B1 is restricted to certain tissues and that the
phorbol myristate acetate - mediated induction of
p84NF - kappa B1 and
p105NF - kappa B1 differs in a cell - type - specific manner . -
pubmed
The
fdxA coding region is 231 nucleotides which codes for a 77
amino acids protein with the molecular weight of 8,744 except for
iron - sulfur clusters . -
pubmed
The 3D structure of a
cyclosporin analogue in water is nearly identical to the
cyclophilin - bound
cyclosporin conformation . -
pubmed
In these polar solvents the conformation is identical and very similar to the structure found in the
cyclophilin -
cyclosporin complex . -
pubmed
The duration of the anovulatory period (
DAP , in d ) was estimated by bi - weekly blood
progesterone measurements . -
pubmed
Twenty - two of the
SLE patients were receiving
corticosteroids (
CS ) at the time of the study . -
pubmed
The critical relative humidity (
CRH ) of the
ranitidine HCl bulk powder was approximately 67 % relative humidity ( RH ) . -
pubmed
Ranitidine HCl powder was unstable around the
CRH . -
pubmed
The lateral shift and increased intensity of
GUS activity in the stem of
rolC::
uidA transformants during dormancy induction was shown to be associated with the accumulation of starch , and to be mimicked by incubating stem sections in
sucrose , as well as
glucose and
fructose , but not
sorbitol , prior to the
GUS assay . -
pubmed
Our results demonstrate that the activities of the
rolC and
35S promoters varied in very different , unpredictable ways during the annual cycle of growth and dormancy in a perennial species , and indicate that the spatial and temporal variation in
rolC promoter activity that we observed in the stem of transgenic hybrid aspen plants is attributable to cellular and seasonal changes in
sucrose content . -
pubmed
In this study , the effects of the deprivation of
macrophage CSF (
M - CSF ) on enriched microglia in cultures were examined by cytochemical methods including in situ nick - end labeling for DNA fragmentation , and Carrazi 's
hematoxylin nuclear staining . -
pubmed
no substantial change in the percentage of students aware of the health risks of
alcohol abuse from Q1 to
Q6 was observed . -
pubmed
Differential actions of
haloperidol on
neurotensin release might be due to regional differences in
dopamine or
sigma receptor subtypes associated with the
neurotensin - containing neurons . -
pubmed
Serum
insulin ,
insulin C - peptide , and
glucose were measured , fasting and 30 min after a 75 - g
glucose load . -
pubmed
The changes observed for fasting
insulin ,
insulin C - peptide ,
glucose , serum lipids and the atherogenic index were also non - significant . -
pubmed
The moths were trained to associate a conditioned stimulus (
CS ) ,
geraniol odour , with an unconditioned stimulus ( US ) , a
sucrose solution . -
pubmed
The aim of the present study was to further investigate the mechanism of the
captopril - induced changes in early LV relaxation by comparing the effects of two
sulphydryl and two non -
sulphydryl containing
ACE inhibitors in the same experimental preparation . -
pubmed
The
sulphydryl - containing
ACE inhibitors
captopril and
zofenaprilat enhanced early LV relaxation , whereas the non -
sulphydryl - containing
ACE inhibitors
lisinopril and
quinaprilat did not . -
pubmed
The effects of
captopril and
zofenaprilat were attenuated both by the
nitric oxide - scavenger
haemoglobin and the
bradykinin B2 - kinin receptor antagonist
HOE 140 . -
pubmed
Neither the
oxygen free - radical scavenger
superoxide dismutase nor the
sulphydryl - containing compound
N - acetyl cysteine administered together with
lisinopril had any effect on LV relaxation . -
pubmed
These data demonstrate that inhibition of intra - cardiac
ACE activity may acutely modulate LV relaxation through increased activity of the
bradykinin -
nitric oxide pathway . -
pubmed
The presence of a
sulphydryl group on the relevant
ACE inhibitor appears to be essential for this LV relaxant effect . -
pubmed
Glucose ( 25 mM ) induces an activation of the transcription of the
fatty acid synthase gene , and this effect is markedly reduced by incubation of the cells with
okadaic acid , an inhibitor of
protein phosphatases 1 and 2A. -
pubmed
A similar reduction in
glucose - activated
fatty acid synthase gene expression is obtained by incubation with
5 - amino - imidazolecarboxamide riboside , a cell - permeable activator of the
AMP - activated protein kinase . -
pubmed
Taken together , these results indicate that the
glucose - induced expression of the
fatty acid synthase gene involves a phosphorylation / dephosphorylation mechanism and suggest that the
AMP - activated protein kinase plays an important role in this process . -
pubmed
Characterization of antibacterial
COOH - terminal
proenkephalin - A - derived peptides ( PEAP ) in infectious fluids . -
pubmed
Importance of
enkelytin , the antibacterial
PEAP209 - 237 secreted by stimulated chromaffin cells . -
pubmed
Finally , we report the high phylogenetic conservation of the
COOH - terminal
PEAP , which implies some important biological function and we discuss the putative importance of
enkelytin in the defensive processes . -
pubmed
Should I be injecting
insulin two or three times a day in an attempt to get very " tight " control of my blood
sugar ? -
pubmed
We looked at the expression of
cyclin D1 in cortical neuronal cultures treated with either
staurosporine to produce apoptotic
death , or with
glutamate , to produce a non - apoptotic
death . -
pubmed
Following the addition of either
staurosporine or
glutamate ,
cyclin D1 immunoreactivity did not change within 4 h. The
cyclin D1 immunoreactivity was lost by 6 h with the appearance of either
staurosporine - induced fragmented nuclei or
glutamate - induced pyknotic nuclei . -
pubmed
The formation of the main band was inhibited by
EDTA , suggesting that this
protease is a
metalloprotease . -
pubmed
METHODS : Dentin disks were treated with : ( 1 ) 35 %
phosphoric acid (
PA ) 20 s ; ( 2 ) PA treatment followed by 1.5 %
NaOCl , 2 min ; ( 3 )
PA treatment , followed by a 10 %
NaOCl immersion for 120 h. SEM was used to analyze the morphology of dentin and its interface with dentin bonding agents ( DBAs ) , while shear bond strength tests were used to measure adhesion . -
pubmed
All specimens were then fractured into two halves : One half was inspected under SEM ; the other half was sequentially placed in 10 %
PA followed by 12.5 %
NaOCl for 70 h , to remove all dentin from the resin replica of the original bonded interface . -
pubmed
RESULTS : SEM observations showed that
collagen fibrils were completely removed from the acid - etched surface by
NaOCl treatment . -
pubmed
In contrast , substantial declines in
Cr concentrations were found in the house dust collected from homes located near the remediated waste sites : previously high - level homes had a median of 50 micrograms / g and mid - level homes had a median of 34 micrograms / g. Site remediation had a beneficial effect on household loadings of
Cr , since no differences in
post - remediation house dust
Cr concentrations were found among the three groups . -
pubmed
Immunological tests showed a normal total
IgE but were negative in the
CAP - FEIA test for
penicilloyl G ,
penicilloyl V ,
amoxycilloyl and
ampicilloyl . -
pubmed
However , a strikingly high frequency of resistance to
piperacillin or
ceftazidime suggests that extended - spectrum
beta - lactamase production in our Enterobacteriaceae may have been more prevalent than realized . -
pubmed
In 14 subjects with leg
DVT we examined the leukocyte filtration [ unfractionated , mononuclear cells (
MN ) , PMNs ] , PMN membrane fluidity and PMN cytosolic
Ca2 + concentration . -
pubmed
The observed J coupling constants were explained by the rigid
sugar conformation model , and the
sugar conformations were found to be between C3'-exo and C2'-endo with phi(m ) values of 26 degrees to 44 degrees , except for the second and 3 ' terminal residues
C2 and
C10 . -
pubmed
The class of drugs known as the
5 - HT3 receptor antagonists have become widely used for chemotherapy - induced
nausea and
vomiting , and are considered a standard part of care for moderately- and highly - emetogenic chemotherapy in combination with
corticosteroids . -
pubmed
The sequence of
full - length mDBR1 cDNA contained a single 515
amino acid open reading frame of 58 kDa protein . -
pubmed
The increased hepatic
corticosterone concentration due to the elevation of
11beta - HSD1 and high plasma
corticosterone concentration may antagonize the action of
insulin and cause
insulin resistance . -
pubmed
A molecular motif required for the activation of
rat neutrophil phospholipase A ( 2 ) by
organochlorine compounds . -
pubmed
PLA ( 2 ) activation stimulated by each of the OC compounds was reduced by
methyl arachidonyl fluorophosphonate , which inhibits both a cytosolic and a
calcium - independent
PLA ( 2 ) ( iPLA(2 ) ) , and by
E-6 -( bromoethylene ) tetrahydro-3 -( 1 - naphthalenyl )- 2H - pyran-2 - one (
BEL ) , a selective inhibitor of
iPLA ( 2 ) . -
pubmed
For an
amino acid sequence XYZ , we define two correction factors , Delta((X)Y)n , s and Delta(Y(Z))n , s , representing the effects on Y 's chemical shifts from the preceding residue ( X ) and the following residue ( Z ) , respectively , where X , Y , and Z are any of the 20 naturally occurring
amino acids , n stands for
( 1 ) H ( N ) ,
( 15 ) N ,
( 1 ) H ( alpha ) ,
( 13 ) C ( alpha ) ,
( 13 ) C ( beta ) , and
( 13 ) C ' nuclei , and s represents the three secondary structural types beta - strand , random
coil , and alpha - helix . -
pubmed
Venous blood samples were drawn for 24 h for assay of total and unbound plasma
ropivacaine ,
3 - hydroxyropivacaine ,
pipecoloxylidide (
PPX ) , and
serum alpha ( 1 )- acid glycoprotein (
AAG ) . -
pubmed
To validate the expression profiling data , we evaluated the protein expression and promoter activity of
prostate - specific antigen (
PSA ) , a gene induced by PC - SPES but repressed by
DES . -
pubmed
We provide evidence that
androgen response elements ( ARE )
II and III within the promoter region are responsible for the suppressive effects of
DES and stimulatory effects of PC - SPES . -
pubmed
In addition , we show that the effects on
PSA transcription are ARE specific in the case of
DES while PC - SPES affects this promoter nonspecifically . -
pubmed
Epsilon - amino caproic acid additive decreases
fibrin bandage performance in a
swine arterial bleeding model . -
pubmed
The
blood loss using the bandage with
EACA (
TC - S+
EACA ; 728.8+/-132 g , n=5 ) was much greater as compared to the
TC - S bandage alone (
TC - S ; 237.8+/-47.9 g , n=6 , p=.02 ) . -
pubmed
The time to " cessation of
bleeding " ( T(b ) ) was also increased for animals treated with
TC - S+
EACA bandages compared to the
TC - S controls ( 33.6+/-10.8 min vs. 9.2+/-2.2 min ; p=.05 ) . -
pubmed
Although plasma
fibrinogen concentration decreased in animals treated with the
TC - S+
EACA bandage , activated partial
thromboplastin times ( aPTT ) and
thrombin times ( TT ) were decreased . -
pubmed
In conclusion , addition of
EACA to the Nycomed product decreased
fibrin bandage efficiency . -
pubmed
Synthetic
oligonucleotides ( ODNs ) are routinely analyzed using capillary gel electrophoresis ( CGE ) for size - sieving based separations as well as electrospray mass spectrometry ( ESI -
MS ) for identification . -
pubmed
Effects of CGE buffer concentration , capillary length , separation and orifice voltage on CGE separation and
MS detection of a
phosphodiester ODN mixture were examined . -
pubmed
The inward current was blocked by the presence of
dihydrokainate ( 0.5 mM ) , shown by others to readily block the
GLT1 isoform . -
pubmed
Reactivation of phosphorylated
acetocholinesterase by
pyridine-2 - aldoxime methiodide . -
pubmed
TARTRATE - INHIBITED
ACID PHOSPHATASE IN
BENIGN PROSTATIC HYPERTROPHY AND IN
PROSTATIC CANCER . -
pubmed
We have investigated the relationship between the induction of
AICD by
phorbol 12 - myristate 13 - acetate plus
ionomycin during the cell cycle and the expression of
survivin , an inhibitor of the
apoptosis protein ( LAP ) family . -
pubmed
Isoprenylcysteine carboxyl methyltransferase modulates endothelial monolayer permeability : involvement of
RhoA carboxyl methylation . -
pubmed
The role of
RhoA and
Rac1 carboxyl methylation in modulating endothelial monolayer permeability is not known . -
pubmed
In this study , we found that inhibition of
isoprenylcysteine - O - carboxyl methyltransferase (
ICMT ) with
adenosine plus
homocysteine or
N - acetyl - S - geranylgeranyl - l - cysteine decreased
RhoA carboxyl methylation ,
RhoA activity , and endothelial monolayer permeability , suggesting that
RhoA carboxyl methylation may play a role in the
ICMT - modulated monolayer permeability . -
pubmed
Similar studies showed no effect of
ICMT inhibition on
Rac1 carboxyl methylation or localization . -
pubmed
PAECs stably overexpressing
ICMT demonstrated increased
RhoA carboxyl methylation , membrane - bound
RhoA , and
RhoA activity . -
pubmed
These effects were blunted by
adenosine plus
homocysteine and by inhibition of
RhoA , but not by inhibition of
Rac1 . -
pubmed
These results indicate that
ICMT modulates endothelial monolayer permeability by altering
RhoA carboxyl methylation and activation , thus changing the organization of intercellular junctions . -
pubmed
Therefore ,
carboxyl methylation of
RhoA may modulate endothelial barrier function . -
pubmed
The structure - activity relationships (
SAR ) for affinity and intrinsic efficacy of analogues of the full agonist
N6 - cyclopentyladenosine (
CPA ) are emphasized . -
pubmed
The
SAR of affinity and intrinsic efficacy of the 2 ' , 3 ' , and especially the
5 '- deoxy derivatives of
CPA is presented . -
pubmed
From this analysis , the nature of the interactions of specific atoms and substituents of the
CPA molecule with the
A ( 1 )- adenosine receptor are deduced and presented pictorially . -
pubmed
At 48 hours after reperfusion , AST ( P = .03 ) and
LDH ( P = .05 ) levels were significantly lower in the
gadolinium group . -
pubmed
Inhibition of
nucleoside transport proteins by
C8 - alkylamine - substituted purines . -
pubmed
4 - nitrobenzylthioinosine (
NBTI , 1 ) is a well - known inhibitor for the
nucleoside transport protein
ENT1 . -
pubmed
The
vitamin K - dependent gamma - carboxylation system in the endoplasmic reticulum membrane responsible for
gamma - carboxyglutamic acid modification of
vitamin K - dependent proteins includes
gamma - carboxylase and
vitamin K 2,3 - epoxide reductase (
VKOR ) . -
pubmed
An understanding of the mechanism by which this system works at the molecular level has been hampered by the difficulty of identifying
VKOR involved in
warfarin sensitive reduction of
vitamin K 2,3 - epoxide to reduced
vitamin K ( 1 ) H ( 2 ) , the
gamma - carboxylase cofactor . -
pubmed
Identification and cloning of
VKORC1 , a proposed subunit of a larger
VKOR enzyme complex , have provided opportunities for new experimental approaches aimed at understanding the
vitamin K - dependent gamma - carboxylation system . -
pubmed
However increased activity of the gamma - carboxylation system , where
VKOR provides the reduced
vitamin K ( 1 ) H ( 2 ) cofactor , was measured only in cells transfected with
VKORC1 and the double transfected cells . -
pubmed
The results show that
VKOR is the rate - limiting step in the gamma - carboxylation system and demonstrate successful engineering of cells containing a recombinant
vitamin K - dependent gamma - carboxylation system with enhanced capacity for
gamma - carboxyglutamic acid modification . -
pubmed
Upregulation of
RPL6 was associated with enhanced resistance to multiple anticancer drugs (
adriamycin ,
vincristine ,
etoposide ,
5 - fluorouracil and
cisplatin ) and to
adriamycin - induced apoptosis . -
pubmed
Downregulation of
RPL6 reversed MDR and sensitized cells to
adriamycin - induced apoptosis . -
pubmed
Alteration of
RPL6 showed no obvious influence on intracellular
adriamycin accumulation ,
glutathione content and expression of
glutathione S - transferase . -
pubmed
A series of
combretastatins substituted with
epoxides ,
amides and small
alkyl groups has been synthesised and evaluated for
cytotoxicity and their ability to inhibit the assembly of
tubulin . -
pubmed
Beyond its antioxidative properties ,
PON1 possesses additional antiatherogenic properties against macrophage foam cell formation : attenuation of
cholesterol and oxidized lipids influx , inhibition of macrophage
cholesterol biosynthesis and stimulation of macrophage
cholesterol efflux . -
pubmed
Nutritional antioxidants , such as
polyphenols , increase
PON1 mRNA expression and activity , by an
aryl hydrocarbon receptor - dependent mechanism . -
pubmed
Some drugs and nutrients including dietary antioxidants and
polyphenols considerably increase the activities of
paraoxonases which , in turn , can reduce oxidative stress and
atherosclerosis development . -
pubmed
Since the cloning of the intracellular
steroid receptors / transcription factors , it has been assumed that most if not all of the actions of the gonadal
steroids are mediated via these intracellular receptors . -
pubmed
In addition , gonadal
steroids can affect second messenger systems , including
calcium and various
kinases to prompt and/or alter cell signaling . -
pubmed
After co - administration of
estradiol and
progesterone ,
progesterone reversed
estradiol 's analgesic effect in Phase
II , however ,
estradiol did not reverse
progesterone 's analgesic activity in Phase I. -
pubmed
To determine if
estradiol effects are receptor - mediated ,
tamoxifen ( selective
estrogen receptor mediator , 15 mg / kg ) or
alpha - estradiol ( an inactive isomer of
estradiol , 20 microg ) were utilized . -
pubmed
Tamoxifen decreased the number of
formalin - induced flinches during Phase
II while
alpha - estradiol did not affect any
formalin - induced responses . -
pubmed
AIM : The aim of this analysis was to examine the average rate and variation of
alcohol - related
injury across emergency department ( ED ) studies , the effect of usual drinking on likelihood of
alcohol - related
injury , whether cross - study variation in rate of
alcohol - related
injury can be explained by between - study difference in usual consumption and whether social - cultural contextual variables help explain cross - study variations , after between - study difference in usual consumption has been controlled . -
pubmed
Two receptors for
ADP , the
G ( q )- protein - coupled P2Y1 and
G ( i )- protein - coupled P2Y12 and one receptor for
ATP , the
P2X1 ion channel , have been identified on platelets . -
pubmed
RESULTS : Compared with control group , the expression of
TERT was increased in 5 micromol / kg
Cd group and 10 micromol / kg
Cd group ,
c - Myc protein was increased in 10 micromol / kg
Cd group , and the expression of
p53 protein was increased in 5 micromol / kg group and 10 micromol / kg
Cd group . -
pubmed
TERT expression in
Se + 10 micromol / kg
Cd group was lower than that of 10 micromol / kg
Cd group significantly . -
pubmed
c - Myc protein was decreased in
Se + 10 micromol / kg
Cd group compared with 10 micromol / kg
Cd group . -
pubmed
p53 protein of
Se + 5 micromol / kg
Cd group and
Se + 10 micromol / kg
Cd group were decreased significantly compared with 5 micromol / kg
Cd group and 10 micromol / kg
Cd group respectively . -
pubmed
Indole , a core nucleus for potent inhibitors of
tubulin polymerization . -
pubmed
Several
tubulin polymerization inhibitors characterized by the presence of an
indole nucleus have been obtained from natural sources or have been prepared by semi - synthesis . -
pubmed
We have reviewed anti -
tubulin agents obtained by synthesis having an
indole as core nucleus . -
pubmed
A high frequency of the
kdr resistance gene has not prevented
pyrethroid - treated nets from functioning , but it is important to develop alternative fabric treatments in case stronger forms of resistance emerge . -
pubmed
We concluded that in all normal subjects their breath test was negative after 3 minutes , and at least a 5 minute wait
post Salamol inhalation is necessary before roadside breath testing should be undertaken , otherwise a failed breath test is likely . -
pubmed
In conclusion , the
MASP1 / 3 gene harbours a low - frequent polymorphic site resulting in an
amino acid substitution , which may influence the function of the gene product . -
pubmed
Neuronal Gq / 11 - coupled dopamine receptors : an uncharted role for
dopamine . -
pubmed
There is strong evidence for the existence of
Gq / 11 - coupled dopamine receptors in the brain but the mechanism by which
dopamine signaling activates
Gq / 11 , or its roles in neuronal function , are only just beginning to be understood . -
pubmed
During apoptosis induction by
sanguinarine and
chelerythrine , reactive
oxygen species ( ROS ) was rapidly generated and DeltaPsi(mt ) dissipated , while
Bax ,
Bcl-2 and
Bcl - X (( L / S ) ) proteins ' content in the mitochondrial fraction did not change significantly . -
pubmed
Caspase-3 activation and DNA fragmentation were considerably inhibited by
N - acetyl - cysteine (
NAC ) . -
pubmed
Chelidonine induced only a slight release of
cytochrome c ( 12h ) , parallel to
caspase-3 activation . -
pubmed
Subtilisin - A was colyophilized with various types of support materials , such as Amberlite IRC-50 , Celite545 , chitosan , DEAE - cellulose , DOWEX-1 ,
zeolite , glass bead , and
polystyrene . -
pubmed
The colyophilized
enzyme was used for the optical resolution of
racemic 1 - phenylethylamine with
2,2,2 - trifluoroethylbutyrate in
3 - methyl-3 - pentanol . -
pubmed
The genes of penile development (
HOX ,
FGF ,
Shh ) and testicular determination (
WT1 ,
SRY ) and those regulating the synthesis [
luteinizing hormone ( LH ) receptor ] and action of
androgen (
5alpha reductase ,
androgen receptor ) can cause
hypospadias if altered . -
pubmed
The cells with impairment of
LDL uptake produced
DCP . -
pubmed
Fat soluble
vitamins are taken up into cells through
clathrin - mediated endocytosis , in which the dynamic polymerization of
F - actin plays a crucial role . -
pubmed
METHODS : Key studies supporting the importance of surrogate markers as diagnostic and prognostic predictors of cardiovascular and renal clinical outcomes in
hypertension , as well as what is known about the effects of
renin -
angiotensin -
aldosterone system - blocking agents on these biomarkers were reviewed . -
pubmed
RESULTS : Clinical data supporting the use of surrogate markers for
heart failure , such as
brain natriuretic peptide (
BNP ) and
N - terminal prohormone BNP ; markers for renal function , such as urinary
albumin to
creatinine ratio ( UACR ) , urinary
albumin excretion rates ( UAER ) , and
creatinine , reflecting glomerular filtration ; and markers of cardiac remodeling , such as
left ventricular hypertrophy and calculations of left ventricular mass index ( LVMI ) , were reviewed for their utility in improving prognosis and treatment efficacy . -
pubmed
CL(int ) values for a cocktail incubation of
phenacetin ,
diclofenac ,
diazepam ,
bufuralol ,
midazolam , and
hydroxycoumarin were 4.9 + /- 3.4 , 18 + /- 7.2 , 5.1 + /- 4.9 ,
6.3 + /- 3.3 , 9.8 + /- 5.8 and 22 + /- 14 microl min(-1)/10(6 ) cells , respectively , and they correlated well with corresponding CL(int ) values using cryopreserved hepatocytes from 25 different donors . -
pubmed
Influence of
Pb ( II ) ions on the
EPR properties of the
semiquinone radicals of
humic acids and model compounds : high field
EPR and relativistic DFT studies . -
pubmed
X - band ( 9.76 GHz ) and high field ( 416.00 GHz ) electron paramagnetic resonance spectroscopy (
EPR ) was used to study the interactions between
Pb ( II ) ions and
semiquinone radicals of natural
humic acids and their simple models . -
pubmed
Principal component analysis ( PCA ) and positive matrix factorization (
PMF ) analysis were followed by multilinear regression analyses to identify and quantify
PAH source contributions , together with spatial and temporal trends . -
pubmed
The
PMF coke oven emission factor showed the greatest variability in total loadings , consistent with the large
PAH emissions from the steel industries and the large influence of wind direction on
PAH concentrations . -
pubmed
The
PMF coke oven impact factor showed good correlations with two commonly used
PAH diagnostic ratios when the ratios were combined into a single ratio . -
pubmed
The immunogen was prepared by linking
MEL hapten with
carrier protein via
carbodiimide method . -
pubmed
The
MAbs showed high specificity with low cross - reactivity ( & lt ; or = 1 % ) toward
cyanurate ,
ammelide , and
ammeline . -
pubmed
PURPOSE :
68Ga - DOTA - DPhe1,Tyr3 - octreotate (
68Ga - DOTATATE ) is a
somatostatin analogue that shows high affinity for
somatostatin receptor subtype 2 (
sst2 ) and has been used for imaging
neuroendocrine tumours . -
pubmed
RESULTS :
68Ga - DOTATATE uptake was noted in the
sst2 - expressing organs such as pituitary , thyroid , stomach wall , spleen , adrenals , kidneys , pancreas and prostate and in the liver and salivary glands , with excreted activity in the bowel and in the pelvicalyceal system of the kidneys and urinary bladder . -
pubmed
We show that the
rpoS allele of
Ty19 (
rpoS(Ty19 ) ) led to the synthesis of a
sigma ( S )( Ty19 ) protein carrying a single
glycine - to -
valine substitution at position 282 in
sigma ( S ) domain 4 , which was much more dependent than the wild - type
sigma ( S ) protein on activation by
Crl , a
chaperone - like protein that increases the affinity of
sigma ( S ) for the
RNA polymerase core enzyme ( E ) . -
pubmed
In addition to
acetylsalicylic acid , a
cyclooxygenase inhibitor ,
ADP - receptor blockers are frequently used for anti - platelet therapy . -
pubmed
Moreover ,
dexamethasone , lipopolysaccharides ( LPS ) and other relevant adipokines such as
leptin and
adiponectin were able to modulate
chemerin ,
LCN2 and
SAA3 mRNA expression alone and when coadministered . -
pubmed
Current evidence supports such a role for the
ABI1 protein
phosphatase and , by inference , protein kinase elements in gating
K (+ ) channel sensitivity to pH(i ) and
ABA . -
pubmed
We describe a robust method for detecting
SHH , WNT , and non -
SHH / WNT molecular subgroups in
formalin - fixed
medulloblastoma samples . -
pubmed
BACKGROUND : The diagnostic accuracy of flat panel digital detector ( FPDD ) cinefluoroscopy for coronary artery
calcium (
CAC ) detection , compared to multiple detector computed tomography ( MDCT ) , is not known . -
pubmed
Median values of urine
IL-8 /
creatinine levels were 61.5 , 4.64 , 15.5 , 14.3 , 1.06 and 4.19 pg / mumol in groups A , B , C , D , E and F respectively . -
pubmed
Taking the backbone of
tenofovir and
lamivudine /
emtricitabine as a reference , we found that , with the exception of regimens including
zidovudine , the effect of other
nucleoside reverse transcriptase inhibitor backbones had little effect on SVR . -
pubmed
In this study , a commercially available
silica - coated paper was explored in an attempt to improve the analysis of therapeutic drugs in dried blood
spots (
DBS ) . -
pubmed
RESULTS : The method used for estimating
glomerular filtration rate was serum
creatinine in 36.6 % ,
creatinine clearance in 41.5 % and equations in 22 % ; none reported using
cystatin - C. -
pubmed
In conclusion , we have demonstrated that a beta - cell specific disruption of the miRNAs network , although allowing for apparently normal beta - cell development , leads to progressive impairment of
insulin secretion ,
glucose homeostasis and
diabetes development . -
pubmed
We now report that
isoflurane anesthesia in young , pre - symptomatic
Tau . -
pubmed
The prescription drug
memantine , identified as an
NMDA receptor antagonist , prevented the post - anesthesia upper airways dysfunction and alleviated
tauopathy in the nucleus ambiguus and Kolliker - Fuse . -
pubmed
Successful resolution of
inflammation and increased regulatory T cells in
sirolimus - treated
post - transplant allograft
hepatitis . -
pubmed
Pharmacological mechanisms of
5 - HT3 and
tachykinin NK1 receptor antagonism to prevent chemotherapy - induced
nausea and
vomiting . -
pubmed
However , in 2003 , a new
5 - HT ( 3 ) receptor antagonist was introduced into the market ; unlike first generation
5 - HT ( 3 ) receptor antagonists ,
palonosetron was found to be effective in preventing both acute and delayed chemotherapy induced
nausea and
vomiting . -
pubmed
Recent mechanistic studies have shown that
palonosetron , in contrast to first generation receptor antagonists , exhibits allosteric binding to the
5 - HT ( 3 ) receptor , positive cooperativity , persistent inhibition of receptor function after the drug is removed and triggers
5 - HT ( 3 ) receptor internalization . -
pubmed
Further , in vitro and in vivo experiments have shown that
palonosetron can inhibit
substance P - mediated responses , presumably through its unique interactions with the
5 - HT ( 3 ) receptor . -
pubmed
ADAM-8 has an overall fold similar to those of other ADAM members , including a central five - stranded beta - sheet and a catalytic
Zn ( 2 + ) ion . -
pubmed
This effect with high BMI might hypothetically be a result of reduced inhibition of
aromatase and suppression of plasma
estrogen levels and might be overcome by the use of an increased dose of
anastrozole or , alternatively , the use of a more potent AI such as
letrozole . -
pubmed
Maximum degradation was observed in undoped and
ZnO - doped beta - TCP samples , which degraded to 41 % and 68 % of the original strength before soaking in
SBF . -
pubmed
Neuropeptide
CART (
cocaine - amphetamine - regulated transcript ) , a hypothalamus and midbrain enriched neurotransmitter with an antioxidant property , can be found in mitochondria , which is the main source of reactive
oxygen species . -
pubmed
In this article , we summarize recent progress in studies investigating the relationship between
CART ,
dopamine , and the pathophysiology of
PD , with a focus on mitochondria - related topics . -
pubmed
Poly ( 3 - hexylthiophene ) (
P3HT ) monolayer has been investigated by scanning tunneling microscopy (
STM ) . -
pubmed
The monolayer was dominated by three kinds of ordered structure ( I ,
II , and III ) , where the
thiophene main chains lied parallel to one another , but high resolution
STM images revealed that the arrangement of the
hexyl side chains was different . -
pubmed
In structure
II , the interchain distance was significantly larger at ~1.52 nm , and the
hexyl side chains were liquid - like . -
pubmed
Structure III exhibited similar interchain distance as structure
II , but the
hexyl side chains were perpendicular to the main chain and were interdigitated . -
pubmed
In addition to these ordered domains , individual
poly ( 3 - hexylthiophene ) chains in various special configurations were observed , and their unfolding into more stable structures was tracked by dynamic
STM , which provides evidence that
P3HT is a relatively flexible polymer . -
pubmed
Altered
ketones suggest that systemic metabolism may play a critical role in preventing
neurological injury , while altered
succinate provides a possible explanation for
hypoxia - inducible factor 1 - alpha (
HIF-1alpha ) stabilization in
HI injury . -
pubmed
We applied photoluminescence ,
PL excitation and absorbance spectra to determine the influence of crystal symmetry , surface to volume ratio and ligands on the optical properties of doped
Eu ( 3 + ) ions . -
pubmed
Unilateral intrastriatal administration of QA ( 300 nmol/4 microl ) significantly reduced
body weight ,
impaired behavior ( locomotor activity , beam balance and memory performance ) , caused oxidative damage ( increased lipid peroxidation ,
nitrite concentration , depleted
super oxide dismutase and
reduced glutathione ) , demonstrated
mitochondrial dysfunction ( decreased
Complex - I , II , III , and IV activities ) , increased striatal lesion volume and altered the levels of
TNF - alpha ,
caspase-3 as well as
BDNF expression , as compared to sham group . -
pubmed
aureus ( hVISA ) using the
glycopeptide resistance detection e - test and population analysis , giving a prevalence rate of
2.19 % . -
pubmed
Pre - administration of
SB334867 , an
orexin-1 receptor antagonist , attenuated the degree of inhibition of swallowing frequency induced by the administration of
orexin - A. -
pubmed
Pre - injection of
SB334867 into the medial DVC disrupted the inhibitory response induced by fourth ventricular administration of
orexin - A. -
pubmed
I
prostanoid receptor - mediated inflammatory pathway promotes hepatic gluconeogenesis through activation of
PKA and inhibition of
AKT . -
pubmed
In this study , we observed dysregulated expression of
cyclooxygenase-2 ,
prostacyclin biosynthesis , and the
I prostanoid receptor (
IP ) in the liver 's response to diabetic stresses . -
pubmed
IP deletion depressed
cAMP - dependent
CREB phosphorylation and elevated
AKT phosphorylation by suppressing
PI3K - gamma /
PKC - zeta - mediated
TRB3 expression , which subsequently downregulated the gluconeogenic genes for
glucose-6 - phosphatase (
G6Pase ) and
phosphoenol pyruvate carboxykinase 1 in hepatocytes . -
pubmed
We exogenously administrated one of three different compounds to adults , that is , ( a )
cortisol , ( b )
glucocorticoid receptor antagonist
mifepristone RU486 or ( c ) sham ( saline ) , and observed their cleaning behaviour during the following 45min . -
pubmed
We also found that the
cytochrome P450 CYP6ER1 was significantly overexpressed in 12
imidacloprid - resistant populations tested when compared with a laboratory susceptible
strain , with fold changes ranging from ten- to 90 - fold . -
pubmed
In contrast , another
cytochrome P450 CYP6AY1 , also implicated in
imidacloprid resistance , was underexpressed in ten of the populations and only significantly overexpressed ( 3.5 - fold ) in a single population from India compared with the same susceptible
strain . -
pubmed
Further selection of two of the
imidacloprid - resistant field
strains correlated with an approximate threefold increase in expression of
CYP6ER1 . -
pubmed
In macrophages the
alpha7 nicotinic acetylcholine receptor (
alpha7nAChR ) modulates production of
inflammatory cytokines ,
cholesterol accumulation and lipoprotein uptake . -
pubmed
Mechanisms through which
omega-3 FA act are manifolds and still a matter of investigation : beside their interaction with ion channel and their influence on plasma membrane fluidity , probably the main effect is acting as competitor for
cytochrome P-450 (
CYP ) with respect to
omega-6 FA . -
pubmed
The aim of the present study is to rationalize
acrylamide pendant Phos -
Tag in - gel discrimination of phosphorylated and non - phosphorylated plant protein species with standard immunoblot analysis , and optimize sample preparation , efficient electrophoretic separation and transfer . -
pubmed
Mucus - buffers - phospholipid layer as pre - epithelial barrier , enhanced by
prostaglandins and
epidermal growth factor , remains a vanguard of mucosal protection . -
pubmed
The surface epithelial cells , elaborating mucins , buffers ,
phospholipids ,
prostaglandins , trefoil peptides ,
peptide growth factor and their receptors ,
heat shock proteins ,
cathelicidins , and
beta - defensins form the second line of defense . -
pubmed
This microcirculation is instrumental for supply of
oxygen ,
nitric oxide ,
hydrogen sulfide and removal of ad hoc generated toxic substances as well as for continuous mucosal cell renewal from progenitor cells , secured by growth factors accompanied by
survivin preventing early apoptosis . -
pubmed
In addition ,
pUL27 is necessary for the full antiviral activity of the
pUL97 kinase inhibitor
maribavir (
MBV ) . -
pubmed
Is
nucleotide exchange sufficient to activate
K - Ras4B ? -
pubmed
With the use of NMR and molecular dynamics ( MD ) simulations , we observed that in solution ,
farnesylated guanosine 5 '- diphosphate (
GDP)-bound
K - Ras4B is predominantly autoinhibited by its hypervariable region ( HVR ) , whereas the
GTP - bound state favors an activated ,
HVR - released state . -
pubmed
In the autoinhibited state , the
HVR is sandwiched between the effector lobe and the membrane ; in the active state , with membrane - anchored
farnesyl and unrestrained
HVR , the catalytic domain fluctuates reinlessly , exposing its effector - binding site . -
pubmed
Notably , we also observe
HVR - autoinhibited
K - Ras4B -
GTP states , with
GDP - bound - like orientations of the helices . -
pubmed
Thus , we propose that the
GDP /
GTP exchange may not be sufficient for activation ; instead , our results suggest that the
GDP /
GTP exchange ,
HVR sequestration ,
farnesyl insertion , and orientation / localization of the catalytic domain at the membrane conjointly determine the active or inactive state of
K - Ras4B. -
pubmed
Importantly ,
K - Ras4B -
GTP can exist in active and inactive states ; on its own ,
GTP binding may not compel
K - Ras4B activation.-Jang , H. , Banerjee , A. , Chavan , T. S , Lu , S. , Zhang , J. , Gaponenko , V. , Nussinov , R. The higher level of complexity of
K - Ras4B activation at the membrane . -
pubmed
The total
bilirubin , direct
bilirubin and
tumor markers (
CA-199 , CA-242 ,
CEA ) before and after surgery , the biliary stent patency status was assessed , and the survival time was evaluated . -
pubmed
With binding to specific
nicotinic acetylcholine receptors ,
nicotine can induce migration and proliferation of vascular cells , and hence enhances angiogenesis . -
pubmed
KEY WORDS : Angiogenesis ;
Atherosclerosis ;
nicotinic acetylcholine receptors (
nAChRs ) ;
Nicotine . -
pubmed
Evolution of
Src Homology 2 ( SH2 ) Domain to Recognize
Sulfotyrosine . -
pubmed
Protein
tyrosine O - sulfation is considered as the most common type of
post - translational
tyrosine modification in nature and plays important roles in extracellular biomolecular interactions . -
pubmed
To facilitate the mapping , biological study , and medicinal application of this type of
post - translational modification , we seek to evolve a small protein scaffold that recognizes
sulfotyrosine with high affinity . -
pubmed
We focused our efforts on the engineering of the
Src Homology 2 ( SH2 ) domain , which represents the largest class of known
phosphotyrosine - recognition domain in nature and has a highly evolvable binding pocket . -
pubmed
The best mutant ,
SH2 - 60.1 , displayed more than 1700 fold higher
sulfotyrosine - binding affinity than that of the wild - type SH2 domain . -
pubmed
Sex hormone - binding globulin regulation of
androgen bioactivity in vivo : validation of the free hormone hypothesis . -
pubmed
Sex hormone - binding globulin (
SHBG ) is the high - affinity binding protein for
androgens and
estrogens . -
pubmed
According to the free hormone hypothesis ,
SHBG modulates the bioactivity of sex
steroids by limiting their diffusion into target tissues . -
pubmed
SHBG increased total
androgen and
estrogen concentrations via hypothalamic - pituitary feedback regulation and prolonged ligand half - life . -
pubmed
Daratumumab (
DARA ) , a monoclonal antibody (
MoAb ) , was developed to treat
relapsed / refractory multiple myeloma (
RRMM ) . -
pubmed
In
chromosome aberration ( CA ) and micronucleus (
MN ) tests ,
roxithromycin did not show
genotoxic effect . -
pubmed
ROS levels were partially reduced by incubation with
PP2 (
c - Src inhibitor ) or
IL1RN , and further reduced by using the
NOX1 / 4 inhibitor
GKT137831 . -
pubmed
Placentas were collected and subjected to histological evaluation , the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene
pleckstrin homology - like domain , family A , member 2 (
PHLDA2 ) , as well as chromatin immunoprecipitation ( ChIP ) for
histone marks within the promoter of
PHLDA2 . -
pubmed
ChIP revealed an increase in the permissive mark dimethylation of
lysine 4 on
histone H3 ( H3K4me2 ) , surprisingly associated with the silent allele of
PHLDA2 , and a decrease in the inhibitory mark H3K9me2 in
NT samples . -
pubmed
PNC-27 is a peptide synthesized of
amino acids of the
p53 -
MDM-2 binding domain . -
pubmed
METHODS : The impact of exposure to
paclitaxel on the surface expression of
MDM-2 was assessed with the use of flow cytometry . -
pubmed
The
cytotoxic effect of
PNC-27 was dependent on its binding to
MDM-2 . -
pubmed
These patients had a reduced transfer coefficient for
carbon monoxide ( Kco ; 33 % [ interquartile range , 30%-35 % ] predicted ) and younger age at diagnosis ( 29 years ; interquartile range , 23 - 38 years ) and more interlobular septal thickening and
mediastinal lymphadenopathy on computed tomography of the chest compared with patients with
PAH without
EIF2AK4 mutations . -
pubmed
The transport of
bile acids facilitated by
NTCP is an important factor in establishing bile flow . -
pubmed
GCDC and
3S - GLC but not
CDCA were transported by
NTCP . -
pubmed
The efficient uptake of
GCDC ,
TCA and
3S - GLC by
NTCP enabled the determination of kinetics . -
pubmed
The apparent CLint value for
GCDC was 20 - fold greater ( 153 + -53mul / mg protein / min ) than the apparent CLint for
TCA ( 6.92 + -4.72mul / mg protein / min ) and apparent CLint for
3S - GLC ( 8.05 + -1.33mul / mg
protein / min ) . -
pubmed
These kinetic results provide important complementary data on the substrate selectivity and specificity of
NTCP to transport
bile acids . -
pubmed
NTCP transports
GCDC with greater efficiency than
TCA and has the same efficacy for
3S - GLC and
TCA . -
pubmed
Computed tomography (
CT ) ,
F - fluorodeoxyglucose (
F - FDG ) , positron emission tomography - computed tomography ( PET -
CT ) , and transthoracic echocardiography showed a space - occupying lesion 7 cm x 5 cm x 5 cm in the right atrium . -
pubmed
Inhibition of
PAK in LA by microinjection of the
PAK inhibitor
IPA-3 30 minutes before fear conditioning enhanced long - term but not short - term fear memory formation . -
pubmed
No significant correlations were found between plasma
AOPP concentration or ImAnOx and
PTH , or their changes in time . -
pubmed
( 2 ) This benefit may be related rather to the direct action of
cinacalcet than to the serum
PTH concentration decrease . -
pubmed
MANF improves the
MPP+/
MPTP - induced
Parkinson ' s disease via improvement of mitochondrial function and inhibition of oxidative stress . -
pubmed
OBJECTIVE : This study aimed to investigate the therapeutic effect of
mesencephalic astrocyte - derived neurotrophic factor (
MANF ) on the
MPTP /
MPP+-induced model of
Parkinson ' s disease (
PD ) and the potential mechanism . -
pubmed
Two weeks later , Rotarod test , immunohistochemistry , and detection of
dopamine ( DA ) and its metabolites ,
superoxide dismutase (
SOD ) ,
glutathione (
GSH ) , and
malondialdehyde (
MDA ) were performed . -
pubmed
MANF pre - treatment significantly decreased the
MPP +-induced reduction of cell viability ( P<0.01 ) , inhibited the ratio of
Bax /
Bcl-2 expression ( P<0.01 ) , activated gene expression levels of
HMOX1 ( P<0.01 ) and
SOD2 ( P<0.05 ) , and reversed
MPP +-induced loss of mitochondrial membrane potential ( P<0.01 ) . -
pubmed
Contrary to
MOG , DNA - histone and
DNA - met - BSA only stimulated the formation of anti - DNA antibodies hydrolysing DNA with a long delay ( 15 - 20 days after immunization ) . -
pubmed
In this study , we designed a
folate - grafted
PEI600 -
CyD (
H1 ) nanoparticle - mediated DNA vaccine containing an adjuvant of absent in
melanoma 2 (
AIM2 ) and a
tumor - specific antigen of
carbonic anhydrase IX (
CAIX ) for
renal carcinoma therapy . -
pubmed
RESULTS : The
GST - NUDT9 - H fusion protein was stabilized with lysis buffer containing 0.5 %
n - dodecyl - beta - d - maltoside (
DDM ) , and wash buffer containing 0.025 %
DDM in size - exclusion chromatography system , and finally the NUDT9 - H with high purity was obtained after cleaved by
thrombin ( 1 U/2 mg fusion protein ) for 24 h. CONCLUSIONS : Due to the poor stability of NUDT9 - H , it is necessary to add
DDM in extraction and purification buffer to stabilize the conformation of NUDT9 - H , so as to increase its yields and purity . -
pubmed
In this review , we will discuss pathways targeted in new topical treatments such as antagonist of
proteinase - activated receptor-2 , the endocannabinoid system ,
neurotrophins and
tropomyosin - related kinase A receptor , the transient receptor potential -
vanilloid or transient receptor potential - melastatine ion channels . -
pubmed
The
skin irritation testing indicated that
CB2 and
CF2 were safe after application and did not cause any
skin irritation . -
pubmed
While
CB2 showed insignificant effects , therefore , it was demonstrated that
CF2 decreased skin
erythema content and increased skin elasticity and hydration significantly but had an insignificant effect on skin melanin content with respect to time . -
pubmed
OBJECTIVE : To assess the safety and efficacy of
crisaborole 2 % ointment - a nonsteroidal ,
phosphodiesterase-4 inhibitor - in the treatment of
intertriginous , anogenital , and facial psoriasis . -
pubmed
Development of an
sEMG sensor composed of two - layered conductive
silicone with different
carbon concentrations . -
pubmed
To achieve robust
sEMG measurements in an EMG prosthetic system , this study proposes a surface electromyogram (
sEMG ) sensor with a novel electrode structure composed of two - layered conductive
silicone with different
carbon concentrations . -
pubmed
We hypothesized there is an optimal
carbon concentration for achieving a large
sEMG amplitude with robustness to external perturbation , and we empirically determined this optimal concentration . -
pubmed
Our results clearly demonstrate an optimal
carbon concentration for robust
sEMG measurements , and suggest that the robust measurement of
sEMG is supported by the capacitance component of the sensor system . -
pubmed
Migrating neurons within the cortical plate of E18.5
Lmnb1 - deficient embryos exhibited
NM ruptures , evident by the escape of the nuclear - localized reporter into the cytoplasm and
NM discontinuities by
electron microscopy . -
pubmed
To test whether mechanical forces on the cell nucleus are relevant to
NM ruptures in migrating neurons , we examined cultured
Lmnb1 -/- neurons when exposed to external constrictive forces ( migration into a field of tightly spaced
silicon pillars ) . -
pubmed
Graft phagocytic cells were depleted in vivo at different time points during
G - CSF /
rapamycin treatment , to identify their role in Tr1 - cell induction and consequently in graft survival . -
pubmed
The role of percentage saturation of
hemoglobin with
oxygen (
oxygen saturation ) , blood pressure and early use of point - of - care ultrasound in differential diagnosis and specific management is detailed . -
pubmed
Prenatal
androgen ( PNA ) exposure mimics
reproductive dysfunction of the common
fertility disorder polycystic ovary syndrome (
PCOS ) and reduces prepubertal
GnRH neuron activity . -
pubmed
Significance Statement
Gonadotropin - releasing hormone (
GnRH ) neuron activity and associated
GnRH release link the neuronal control of reproduction to peripheral secretion of reproductive hormones . -
pubmed
Here , we reveal that prepubertal suppression of GnRH neuron activity without
androgen exposure leads to similar increases in adult
GnRH neuron activity , and a mild degradation in reproductive cycles . -
pubmed
This provides a clearer understanding of the role prepubertal
GnRH neuron activity plays in establishing adult reproductive function and suggests additional
androgen - dependent programming actions are required for complete reproductive disruption in this model and perhaps
PCOS . -
pubmed
All isolates were multidrug resistant but
vancomycin susceptible ; they carried the
icaA gene but tested negative for the pvl and
ses genes . -
pubmed
Here we set out to characterize the expression status of
LINC00426 in
osteosarcoma and understand its mechanistic involvement in incidence of
doxorubicin (
Dox ) resistance . -
pubmed
The direct association between
LINC00426 and miR-4319 was analyzed by pulldown assay with
biotin - labelled probes . -
pubmed
RESULTS :
LINC00426 was significantly up - regulated in
Dox - resistant
osteosarcoma ( OS ) both in vitro and in vivo , which intimately associated with unfavorable prognosis . -
pubmed
SiRNA - mediated knockdown of LINC00426 remarkably compromised cell viability and proliferation in
Dox - resistant OS cells , which accompanied with decrease of IC50 and activation of
caspase-3 . -
pubmed
CONCLUSION : Ectopic introduction of miR-4319 significantly surmounted the
Dox resistance in
OS cells , while miR-4319 inhibition in
LINC00426 - deficient cells greatly restore this phenotype . -
pubmed
We uncovered the important contribution of
LINC00426 / miR-4319 to
Dox resistance in
osteosarcoma . -
pubmed
We test hypothesis whether high
methionine diet (
HMD , an
amino acid high in red - meat ) a substrate for
Hcy can cause skeletal muscle and
growth retardation and treatment with probiotics (
PB ) mitigate
muscle dysfunction . -
pubmed
A greenhouse experiment as a completely randomized design with six treatments of bio / organic - fertilizers ( no bio - fertilizer ( NF ) , RHB ,
RHC , MR , RHB+MR , and
RHC+MR ) and three levels of
SDZ application ( 0 , 100 , and 200 mg kg-1 ) was performed for 7months with three replicates . -
pubmed
The results revealed that the application of
RHC , RHB , and MR had a significant impact on the reduction of the
toxicity effects of
SDZ on plant properties . -
pubmed
The same results were obtained with macro (
NPK ) and micro (
Fe ,
Zn ,
Cu , and
Mn ) nutrients uptake by the shoot in which the lowest values of nutrients uptake were observed in treatment of 200 mg kg-1 of
SDZ with no bio - fertilizers . -
pubmed
And the characterization of
BcHep - I after purified with
Ni - NTA affinity chromatography suggested that it is a mesophilic enzyme . -
pubmed
BcHep - I can be activated by 1 mM
Ca2 + ,
Mg2 + , and
Mn2 + , while severely inhibited by
Zn2 + ,
Co2 + , and
EDTA . -
pubmed
These aspects have motivated the search for collectors that exhibit superior performance without the health , safety and environmental (
HSE ) concerns associated with
xanthates . -
pubmed
3 -( 2,4,6 - trinitrophenylhydrazone -( methyl ) rifamycin SV is a strong inhibitor for all the
polymerases tested and belongs to the C class inhibitors of
reverse transcriptase . -
pubmed
From protective action of the template - primer and other data it might be suggested that the
rifamycin derivatives act at an early step(s ) in DNA synthesis catalyzed by
reverse transcriptase . -
pubmed
Trypsin was then covalently bonded onto the
CMPS membrane by diazotization . -
pubmed
The activity of immobilized
trypsin reaches up to 10200 U / g ; 15 mg
trypsin was immobilized on 1 g
CMPS membrane . -
pubmed
The two forms of
stromelysin were subsequently separated , again using
Procion Red - Sepharose . -
pubmed
The
acridine half - mustard ICR-170 - induced reversion of the mutant
am15 , which has a single base - pair deletion , at a frequency of between 9 and 28 X 10(-6 ) . -
pubmed
The purified
MAb was found to be over 95 % pure based on the results from
SDS - PAGE . -
pubmed
The role of the activated
c - H - ras-1 locus in the initiation of
mammary neoplasia was analysed by studying seven transplantable ,
DMBA - induced Balb / c premalignant
mammary hyperplasias . -
pubmed
Modulation by
protein kinase C but not by
cyclic nucleotides . -
pubmed
Protein kinase C activators attenuated
GnRH - induced
phosphoinositide hydrolysis , while neither
cyclic AMP analogs nor
cyclic GMP analogs were effective . -
pubmed
These results indicate that
phosphoinositide hydrolysis represents an important postreceptor transducing mechanism for
GnRH action at the gonadotroph and that
protein kinase C ( but not
cyclic nucleotides ) may exert a negative feedback control on
GnRH receptor - coupling mechanisms . -
pubmed
Linkage and segregation analyses of
apolipoproteins A1 and B , and lipoprotein
cholesterol levels in a large pedigree with excess
coronary heart disease : the Bogalusa Heart Study . -
pubmed
Robust methods were employed , using data from a single large pedigree , to screen serum
apolipoprotein A1 and B levels ,
serum lipoprotein cholesterol levels , and ratios of serum lipoprotein
cholesterol fractions to
apolipoprotein A1 and B levels for genetic linkage to 31 polymorphic markers . -
pubmed
Segregation analyses were performed for each of the
apolipoprotein and lipoprotein
cholesterol fractions to obtain estimates for use in applying likelihood methods of linkage analysis . -
pubmed
Results from the segregation analyses were consistent with major gene determination of
apo B and
HDL - C levels , the
HDL - C to
apo A1 ratio , the
LDL - C to
apo B ratio , and a measure of relative content of
cholesterol in
HDL - C and
LDL - C. Linkage between
haptoglobin and the
HDL - C /
apo A1 ratio was suggested , with a lod score of 1.72 at theta = 0.05 . -
pubmed
Indoxyl sulfate could not be removed effectively by conventional hemodialysis because of its strong binding to
serum albumin . -
pubmed
Equilibrium dialysis demonstrated that
indoxyl sulfate inhibited the binding of
salicylate to
albumin , and that
3 - carboxy-4 - methyl-5 - propyl-2 - furanpropionic acid inhibited the binding of
indoxyl sulfate to
albumin . -
pubmed
The review provides a conclusive evidence which suggests that
chromium , especially its hexavalent form , is both toxic and carcinogenic , but its trivalent form is physiologically essential in the metabolism of
insulin . -
pubmed
Mutations at the
chlA or
chlE locus , which are required for
molybdenum cofactor biosynthesis , similarly do not affect the activity of the heat - treated extract in the in vitro activation process . -
pubmed
None of the other known pleiotropic
chlorate resistance loci (
chlD ,
chlG ) are required for the expression of its activity . -
pubmed
We conclude that a low - molecular - weight substance , distinct from the active
molybdenum cofactor , is required to bestow activity on the
molybdoenzyme nitrate reductase during its biosynthesis . -
pubmed
Tryptic degradation of the 50 K fragment abolished , or reduced greatly , the binding of heat - treated subfragment 1 to
actin in solution but not on
nitrocellulose overlays . -
pubmed
It is likely that during its differentiation the primary mesenchyme provides some of its own extracellular microenvironment in the form of
collagen and
proteoglycans . -
pubmed
Injected neurons were first examined in whole slices , and subsequently , in sectioned material , characterized immunocytochemically using antisera to
vasopressin- and
oxytocin - associated
neurophysins (
VP - NP and
OT - NP respectively ) . -
pubmed
Six of the single cells and all the
dye coupled pairs were immunoreactive with
VP - NP antiserum and not reactive with
OT - NP antiserum . -
pubmed
[ Dentin liquid : determination of
creatinine and
lactate dehydrogenase content ] . -
pubmed
Antagonistic effects of
25 ( OH ) vitamin D3 and peptide hormones on the activation of
adenylate cyclase /
cyclic AMP system in renal tissue in vitro . -
pubmed
[ Mechanism of the anti - aggregation action of
thyroxine and
insulin ] . -
pubmed
Thyroxine and
insulin inhibit the aggregation capacity of platelets . -
pubmed
Based on the data obtained a hypothesis is advanced according to which
thyroxine inhibits the
platelet aggregation , interfering with the release of
arachidonic acid from
phospholipids of platelets (
thrombocytopathy A ) , whereas
insulin -- by blocking the
cyclooxygenase activity (
thrombocytopathy B ) . -
pubmed
Analyses of the
tubulin by two - dimensional
polyacrylamide gel electrophoresis , isoelectric focusing , and peptide mapping indicated that the beta - tubulin complex remained constant regardless of source but that some heterogeneity was present in the alpha subunit . -
pubmed
After 120 minutes of incubation , however ,
Tris inhibited the conversion of
proPTH to parathormone (
PTH ) , judged by a decrease in cellular and secreted radioactive
PTH and a corresponding increase in radioactive cellular
proPTH . -
pubmed
With both concentrations of
A23187 and the low concentration of
X537A there were no changes in amounts of radioactive
proPTH , moderate decreases in cellular and secreted radioactive
PTH , and little discernible distension of the Golgi membranes . -
pubmed
At 17 microM
X537A there was moderate increase in amount of radioactive
proPTH , a marked decrease in amount of radioactive
PTH and swelling of the Golgi membranes . -
pubmed
Taken together , these findings suggest that
Tris inhibited conversion of
proPTH to
PTH by disrupting the Golgi zone - the site of conversion of
proPTH to
PTH ; that
A23187 and the low concentration of
X537A decreased production of
PTH by enhancing its degradation ; and that
X537A at the higher concentration acted both by inhibiting conversion of
proPTH to
PTH and by enhancing the degradation of
PTH . -
pubmed
The effects of chronic
morphine administration were tested in a paradigm of 7 days saline , 7 days
morphine , 1 day
morphine+
naloxone , and 6 days
post - drug saline . -
pubmed
[ The
renin -
aldosterone profile in
hypertension ] . -
pubmed
Moderate positive correlation was observed between the level of
aldosterone and
renin activity ;
aldosterone - renin index at the stage of the disease remains unchanged . -
pubmed
MM -
MTX were prepared by the following methods : ( A ) reaction of
MTX with
poly ( ethylene glycol ) 1500 (
PEG ) to form a
poly ( ethylene glycol )- methotrexate conjugate (
PEGMTX ) which was then added to a
ferrous /
ferric ion salt solution to give MM - MTX I ; ( B ) reaction of
ferrous /
ferric ion salts with
PEG to give a ferromagnetic polymer complex which was then coupled with
MTX to give MM - MTX
II ; ( C ) MM - MTX IIIA were prepared by reacting
MTX with
amino - terminated magnetic microspheres , commercially available , in the presence of
1 - ethyl-3,3 - bis ( methylamino ) propylcarbodiimide (
EDCI ) ; ( D ) reaction of
aminohexanol with
di - tert - butyl dicarbonate to form an
[ N -( tert - butoxycarbonyl ) amino ] hexanol (
t - Boc - AH ) , which was then coupled with
MTX in the presence of
1,3 - dicyclohexylcarbodiimide and
4 - pyrrolidinopyridine to give a
t - Boc - AH - MTX conjugate , which was then saturated with
hydrogen chloride to give an
aminohexanol -
methotrexate (
AH - MTX ) conjugate . -
pubmed
Drug content of the magnetic microsphere -
methotrexate conjugates as determined by HPLC was 0.45 % ( w / w ) , 4.0 % ( w / w ) , and
6.3 % ( w / w )
MTX for
MM - MTX I , MM - MTX
II , and
MM - MTX IIIB , respectively. -
pubmed
Aryl hydrocarbons (
AHs ) such as
2,3,7,8 - tetrachlorodibenzo - p - dioxin and
benzo [ a ] pyrene activate the sequence - specific DNA - binding activity of the
AH receptor . -
pubmed
Methylamine protection experiments and immunofluorescence studies suggested that
CNF2 enters the cytosol of the target cell through an acidic compartment and induces the reorganization of
actin into stress fibers . -
pubmed
The [
32P]
ADP - ribosylated
Rho proteins from
CNF2 - treated cells migrated slightly more slowly in
SDS / PAGE than did the labeled proteins from the control cells . -
pubmed
PROCEDURE : Colonic smooth muscle strips were suspended in physiologic buffer solution , attached to isometric force transducers , and contracted with
acetylcholine (
ACh ; 10(-9 ) to 10(-4)M ) ,
substance P ( SP ; 10(-10 ) to 10(-6)M ) ,
cholecystokinin (
CCK ; 10(-11 ) to 10(-8)M ) ,
potassium chloride (
KCl ; 10 to 80 mM ) , or electrical field stimulation (
EFS ; 25 V , 1 to 30 Hz , 0.5 - millisecond duration ) . -
pubmed
RESULTS : Passive isometric stress was not altered , but the active isometric stress responses of
megacolon smooth muscle to
ACh , SP ,
CCK ,
KCl , and
EFS were significantly ( P & lt ; 0.05 ) diminished , compared with healthy controls . -
pubmed
The contractile response of
megacolon smooth muscle to
EFS , and the inhibition of this response by
tetrodotoxin , suggest that myenteric and submucosal plexus neurons in
megacolon smooth muscle are functional . -
pubmed
The diminished isometric stress responses to receptor occupancy (
ACh , SP , and
CCK ) and membrane depolarization (
KCl ) further suggest that the disorder involves disturbance in the activation of smooth muscle myofilaments . -
pubmed
After 23 d of treatment , concentrations of
ACTH and
cortisol were measured during 7.5 h. On d 24 ,
cortisol concentrations were determined at time -.5 , 0 , .5 , 1 , 2 , 3 , 4 , and 5 h after i.v . -
pubmed
Cortisol concentrations did not differ among treatment groups in the baseline series and after
ACTH administration . -
pubmed
[ Normal cerebral perfusion of
99mTc -
ECD brain SPECT -- evaluation by an anatomical standardization technique ] . -
pubmed
Therefore , the specific purpose of this study was to investigate the normal distribution pattern of
99mTc -
ECD SPECT image . -
pubmed
The primary structure of
barley grain serpin BSZ7 was deduced from a cDNA encoding 397
amino - acid residues . -
pubmed
Functional integrity of hepatocytes was evaluated from
lactate dehydrogenase (
LD ) leakage ,
urea synthesis in the perfusion medium and lipid
peroxides (
LP ) formation . -
pubmed
Low concentration of
sodium nitroprusside ( as
NO donor ) significantly decreased
LD leakage , increased the rate of
urea synthesis and increased
trypan blue exclusion by hepatocytes in
anoxia / reoxygenation or
cumene hydroperoxide injured ( 0.2 mM ) cells . -
pubmed
Using these methods , we investigated the effects of the sulphated octapeptide of
cholecystokinin (
CCK8 ) on gastric emptying and on stomach motility in vivo and in vitro . -
pubmed
The decrease in amplitude was not affected by the
nitric oxide synthase inhibitor
NG - nitro - l - arginine methyl ester hydrochloride (
L - NAME ; 10(-4 ) mol l-1 ) . -
pubmed
Depending on the concentration and experimental arrangement ,
CCK8 had either inhibitory or excitatory effects on the cardiac stomach , suggesting the possible presence of different types of
CCK receptor . -
pubmed
Increasing plasma
neopterin and persistent plasma
endothelin during follow - up after acute
cerebral ischemia . -
pubmed
Plasma
neopterin was higher at follow - up (
6.3 nmol / L [ 3.7 - 21.6 ] vs 5.6 nmol / L [ 3.5 - 17.2 ] ; p & lt ; 0.05 ) than at the acute stage , whereas the plasma
ET-1 concentration was unchanged . -
pubmed
Plasma concentrations of both
neopterin and
ET-1 correlated directly with age both in the acute stage ( r = 0.42 and r = 0.35 , respectively ; p & lt ; 0.01 ) and after follow - up ( r = 0.34 ; p & lt ; 0.05 and r = 0.27 ; p = 0.05 , respectively ) . -
pubmed
Linomide downregulates autoimmunity through induction of TH2
cytokine production by lymphocytes . -
pubmed
In this study , in an effort to elucidate the mechanisms by which
Linomide suppresses
EAE , and
autoimmunity in general , we investigated the in vivo effects of this drug on the
TH1 / TH2 lymphocyte balance , which is important for the induction or inhibition of autoireactivity . -
pubmed
A significant upregulation of
IL-4 ,
IL-10 and
TGFbeta was observed following treatment with
Linomide , which peaked at day 10 (
IL-10 ) or day 15 (
IL-4 ) . -
pubmed
We speculate that
Ihyp - act may have a role in basal
Cl- absorption , acting as a
Cl- sensor to maintain optimal volume and composition of airway surface liquid . -
pubmed
The new technique - acid coinjection - achieves a similar retention enhancement for
arginine with only a minimal effect on the
rest of the separation . -
pubmed
The
Al ( 3 +)-activated current exhibited higher permeability to
malate(2- ) than to
Cl (- ) ( P ( mal )/ P ( Cl ) & gt ; or = 2.6 ) and was inhibited by
anion channel antagonists ,
niflumate and
diphenylamine-2 - carboxylic acid . -
pubmed
Antidiuretic action of
vasopressin : quantitative aspects and interaction between
V1a and V2 receptor - mediated effects . -
pubmed
( 1 )
Vasopressin (
VP ) , or
antidiuretic hormone , is secreted in response to either increases in plasma osmolality ( very sensitive stimulus ) or to decreases in plasma volume ( less sensitive stimulus ) . -
pubmed
( i ) Increase in
water permeability along the entire CD ( via
AQP2 ) . -
pubmed
( 3 ) Because urine is first diluted along the nephron before being concentrated in the medulla ,
VP is required , not only for urine concentration , but first for re - equilibration of tubular fluid osmolality with plasma osmolality , a step taking place in the renal cortex , and achieved through the reabsorption of large quantities of
water ( more than what is subsequently reabsorbed in the medulla to concentrate urine ) . -
pubmed
Surface expression of
P - selectin was minimal in unstimulated HBMEC ; however , it was significantly augmented upon stimulation with
histamine ( 10(-7)-10(-3 ) M ) and
thrombin ( 0.01 - 1 U / ml ) . -
pubmed
Histamine - induced surface expression of
P - selectin was blocked by the
histamine H2 receptor antagonist
cimetidine . -
pubmed
The
H1 receptor antagonist
mepyramine had no effect . -
pubmed
Expression was reduced by the extracellular
calcium chelator
EDTA and blocked by the
cyclic AMP phosphodiesterase inhibitor
rolipram . -
pubmed
Thus
histamine and
thrombin both increase
P - selectin expression in HBMEC . -
pubmed
Histamine mediates expression through the
H2 , but not the
H1 , receptor and
calcium , whereas expression is reduced by
cyclic AMP . -
pubmed
RESULTS : Five mutations in the beta -
globin gene which are common in the Indian population were detected in 92.3 % of mutant chromosomes , whereas 3.1 % of chromosomes carried rare mutations followed by 0.8 %
haemoglobin E and 0.4 % sickle cell mutations . -
pubmed
In order to evaluate the ability of multivalent
glycosides based on a beta - cyclodextrin core as site - specific molecular carriers , a study on both the inclusion complexation behaviour and
lectin binding affinity of branched and hyperbranched beta - cyclodextrins is presented . -
pubmed
The persubstitution of the primary
face of the beta - cyclodextrin with
saccharides led to a slight increase of the binding constant values for the inclusion complexation with ANS relative to the native beta - cyclodextrin . -
pubmed
METHODS : RBC extracts were trimethylsilylated (
TMS ) and analyzed by gas chromatography / mass spectrometry (
GC /
MS ) . -
pubmed
In the whole sample , multiple regression analysis of dose - normalized concentration revealed significant effects of time postdose ( -18 % per 12 hours delay , P & lt ; 0.05 ) , age & gt;/=60 years ( +27 % , P & lt ; 0.005 ) , cigarette smoking ( -12 % , P & lt ; 0.05 ) , and comedication with
fluvoxamine ( +74 % , P & lt ; 0.001 ) ,
paroxetine ,
fluoxetine , or
sertraline ( considered together , +32 % , P & lt ; 0.05 ) ,
venlafaxine ( +27 % , P & lt ; 0.05 ) , and inducers of
P450 enzymes ( -40 % , P & lt ; 0.001 ) . -
pubmed
We investigated differentiation of
CD4 T cells responding to
Ag presented by bone marrow - derived dendritic cells ( DC ) in association with
MHC class II (
MHC II ) molecules . -
pubmed
MHC II - peptide - specific T cells responding to this
Ag were polarized to a Th1
cytokine profile in a
CD40- ,
CD28- ,
MyD88- , and
IL-12 - dependent manner . -
pubmed
DC that took up the same
FcR - targeted liposomes and then were exposed to
methyl - beta - cyclodextrin , which chelates
cholesterol and dissociates lipid microdomains , also stimulated Th2 differentiation . -
pubmed
They caused a severe decrease of the respiratory function and altered the assembly of the
iron - sulfur protein in the
bc ( 1 ) complex , as observed by immunodetection . -
pubmed
Suppressor mutations that partially restored the respiratory function impaired by S152P or G291D were found in or close to the hinge region of the
iron - sulfur protein , suggesting that this region may play a role in the stable binding of the subunit to the
bc ( 1 ) complex . -
pubmed
Y279C caused a significant decrease of the
bc ( 1 ) function and perturbed the
quinol binding . -
pubmed
The
ankyrin repeat is one of the most frequently observed
amino acid motifs in protein databases . -
pubmed
More significantly , our data demonstrate that in vivo treatment with the
petroleum ether extract exerts an
insulin - sensitizing action by enhancing the activity of the two major intracellular signal transduction pathways of the hormone 's receptor . -
pubmed
Heparin was infused at a rate of 16 - 32 IU/ kg / h to maintain an
ACT of 160 - 180 seconds . -
pubmed
We have previously reported that
YO-2 , a selective
plasmin inhibitor , induces thymocyte apoptosis . -
pubmed
To elucidate the mechanism of
YO-2 - induced apoptosis , other YO compounds with different
plasmin inhibitory action were tested for the pro - apoptotic activity in this study . -
pubmed
Lactams as
EP4 prostanoid receptor agonists . -
pubmed
The latter
lactams were devised using a pharmacophore for
EP ( 4 ) receptor activity . -
pubmed
gamma - Lactams were characterized for their
prostanoid EP receptor affinities and EP(4 ) activity and found to be selective for the
EP ( 2 ) and
EP ( 4 ) receptors or selective for the
EP ( 4 ) subtype . -
pubmed
Women with
PCOS and the
MBS had significantly higher levels of serum free
testosterone ( P = 0.002 ) and lower levels of serum
SHBG ( P = 0.001 ) than women with
PCOS without the
MBS . -
pubmed
In this analysis of the charge distribution of 7
proteins , we estimate the solvation free energy contribution to the total free energy by exploring the 2(zeta ) possible ionization states of the whole molecule , with zeta being the number of ionizable groups in the
amino acid sequence , for every conformation in the ensembles of 7
proteins . -
pubmed
CONCLUSION : These results suggest that exercise training induces the altered expression in several genes including
prostaglandin ,
CNP , and
nitric oxide in the aorta and that these molecular changes ( particularly
eNOS as its protein expression was altered ) may contribute , at least in part , to the beneficial effect of exercise training on aortic stiffness . -
pubmed
Both cell lines were refractory to
Fas ; whereas ,
alpha - TEA sensitized them to
Fas signaling . -
pubmed
alpha - TEA increased levels of
Fas message , protein and membrane - associated
Fas . -
pubmed
Neutralizing antibodies to
Fas or
Fas L partially blocked
alpha - TEA - induced apoptosis . -
pubmed
alpha - TEA induced prolonged activation of
c - Jun N - terminal kinase (
JNK ) and its substrate
c - Jun ;
Bax conformational change ; and cleavage of
Bid and
caspases-8 , - 9 and - 3 . -
pubmed
Chemical inhibitors of
JNK , and
caspases blocked
alpha - TEA - induced apoptosis . -
pubmed
alpha - TEA decreased phosphorylation of
protein kinase B (
Akt /
PKB ) and
extracellular signal - regulated kinase (
ERK1 / 2 ) , as well as cellular
FLICE - like inhibitory protein (
c - FLIP ) and
Survivin protein levels . -
pubmed
Knockdown of
Akt and
ERK activity using
phosphoinositide - 3 - kinase (
PI3 K ) and
mitogen - activated protein kinase kinase (
MKK1 ) inhibitors enhanced
alpha - TEA - induced apoptosis . -
pubmed
Over - expression of constitutively active
Akt2 and
MKK1 blocked
alpha - TEA - induced apoptosis . -
pubmed
Considering the importance of
nitric oxide generation in the regulation of vessel tone , reduced
endothelial nitric oxide synthase (
eNOS ) expression in alveolar macrophages exposed to short - term
silica (
Si ) suggests the possibility of
Si - induced changes in endothelial functions . -
pubmed
Si - induced reduced
NO association with elevated
endothelium - derived hyperpolarizing factor (
EDHF ) in response to
ACh , together with reduced
NO sensitization , might have clinical importance in cardiovascular pathology . -
pubmed
In summary , brain
VIP and
PACAP both appear to exert generally catabolic effects on energy metabolism in the chick , but their influence on body temperature and
glucose metabolism differs and their central effects do not appear to be mediated by the same receptors . -
pubmed
Studies were conducted on day 20 , when blood was collected for
corticosterone measurement as an indication of hypothalamus - pituitary - adrenal (
HPA ) axis activity , and colonic function was studied in tissues mounted in Ussing chambers . -
pubmed
Pulmonary neuroendocrine cells ( PNEC ) , including neuroepithelial bodies (
NEB ) , are
amine- and peptide ( for example ,
bombesin)-producing cells that function as
hypoxia /
hypercapnia - sensitive chemoreceptors that could be involved in the pathophysiology of
sudden infant death syndrome (
SIDS ) . -
pubmed
Before differentiation , significantly more cells incorporated
BrdU in cultures exposed to
FGF2 ( 19 - fold ; P & lt ; 0.001 ) and
FGF8 ( 3 - fold ; P & lt ; 0.05 ) compared to controls . -
pubmed
After differentiation , biochemical analyses showed significantly more
dopamine and
FA1 in conditioned medium from both
FGF2 and
FGF8 expanded cultures than in controls . -
pubmed
Glucoamylase is an industrially extremely important enzyme in the fermentative production of
ethanol , used in the enzymatic conversion of starch into high
glucose and
fructose syrups . -
pubmed
Induction by 0.75 %
methanol for 72 h led to synthesis of secreted
glucoamylase . -
pubmed
Based on the activity profiles and the availability of materials , five inhibitors were examined for their ability to compete with
[ ( 125 ) I ] iodoarylazidoprazosin labeling of
ABCG2 , increase binding of the anti -
ABCG2 antibody 5D3 , and prevent
P - glycoprotein or
multidrug resistance protein 1 - mediated transport . -
pubmed
None of the compounds affected
P - glycoprotein - mediated
rhodamine 123 transport , whereas three affected
multidrug resistance protein-1 - mediated
calcein transport at 25 mumol / L , suggesting that the compounds are relatively specific for
ABCG2 . -
pubmed
Isoprostanes are
prostaglandin - like bioactive compounds that are biosynthesized in vivo independent of
cyclooxygenases , principally through free - radical catalyzation of
arachidonic acid . -
pubmed
Effects of
urea on protein stability have been studied from all - atom molecular dynamics simulations of
ubiquitin , G311 protein , and
immunoglobulin binding domain (
B1 ) of
streptococcal protein G (
GB1 ) in water and 8 M aqueous
urea solution . -
pubmed
The mechanism of the change in the solvent environment and the early events in protein unfolding by
urea have been identified with emphasis on the change in the interactions of hydrophilic and hydrophobic parts of the
protein by calculating the potential of mean force (
PMF ) . -
pubmed
Urea replaces the protein - protein and protein - water contacts by forming stronger contacts with the
protein , which is indicated by the longer survival times of the protein -
urea hydrogen bonds . -
pubmed
Crystalline structure of
ceria particles controlled by the
oxygen partial pressure and
STI CMP performances . -
pubmed
The effect of the crystalline structures of nano - sized
ceria particles on shallow trench isolation (
STI ) chemical mechanical planarization (
CMP ) performance was investigated . -
pubmed
The crystalline structures of
ceria particles were analyzed by the high - resolution
TEM nano - beam diffraction pattern . -
pubmed
In the
STI CMP evaluation , the
ceria slurry prepared with the cubic
CeO ( 2 ) shows enhanced performances of the
oxide - to -
nitride removal selectivity . -
pubmed
The severity of
cholestasis and
hepatic injury was determined by changes in the plasma enzyme activities of
aspartate aminotransferase ,
alanine aminotransferase ,
gama glutamin transferase , and levels of total
bilirubin and direct
bilirubin . -
pubmed
Malondialdehyde ,
nitric oxide , and
superoxide dismutase were determined to evaluate the oxidative status in the liver tissue . -
pubmed
Myeloperoxidase activity and levels of tissue
hydroxyproline were determined to assess neutrophil activation and
collagen accumulation , respectively . -
pubmed
Sphingosylphosphorylcholine also inhibited the increase in liver
malondialdehyde ;
nitric oxide levels significantly and also attenuated the depletion of
superoxide dismutase in the liver after BDL . -
pubmed
Similarly , the increase in tissue
myeloperoxidase activity and
hydroxyproline owing to BDL was also attenuated by the
SPC treatment . -
pubmed
Although one antagonist --
maraviroc , a
CCR5 inhibitor to treat
AIDS -- has been registered as an approved drug , this is the only success so far . -
pubmed
This paper presents a
GC -
MS analytical procedure for determining proteinaceous materials ,
glycerolipids , natural waxes and
terpenoid resins in the same paint micro - sample . -
pubmed
The proteinaceous fraction is analysed by
GC -
MS after hydrolysis and derivatisation of the freed
amino acids . -
pubmed
Drugs with a strong rationale that have failed or have not shown univocal results in clinical trials so far include
eicosapentaenoic acid ,
cannabinoids ,
bortezomib , and anti -
TNF - alpha MoAb . -
pubmed
Several emerging drugs have shown promising results but are still under clinical investigation (
thalidomide , selective
cox-2 inhibitors ,
ghrelin mimetics ,
insulin ,
oxandrolone , and
olanzapine ) . -
pubmed
During subunit assembly , this segment of the
chaperone is displaced by an
amino - terminal extension of an incoming subunit in a reaction termed donor - strand exchange . -
pubmed
Here we show that the binding of complexes between
FimC and the different pilus subunits to the
amino - terminal substrate recognition domain of
FimD is an extremely fast process , with association rate constants in the range of 10(7)-10(8 ) M(-)(1 ) s(-1 ) at 20 degrees C. Furthermore , we demonstrate that the ordered assembly of pilus subunits is a consequence of the usher 's ability to selectively catalyze the assembly of defined subunit - subunit pairs that are adjacent in the mature pilus . -
pubmed
Water -- more than just a green solvent : a stereoselective one -
pot access to all - chiral
tetrahydronaphthalenes in aqueous media . -
pubmed
Using rationally designed substrates , the reaction conditions have been optimized and the one -
pot process has been applied to a series of
nitroolefin acrylates and
aldehydes . -
pubmed
Here we summarize evidence that the acute actions of the
steroid arise from
actin signaling cascades centrally involved in long - term potentiation ( LTP ) . -
pubmed
E2 's actions on transmission and plasticity were completely blocked by
latrunculin , a toxin that prevents
actin polymerization . -
pubmed
Estrogen activated the
small GTPase RhoA , but not the related
GTPase Rac , and phosphorylated ( inactivated ) synaptic
cofilin , an actin severing protein targeted by
RhoA. -
pubmed
Collectively , these results indicate that
E2 engages a
RhoA &
gt;
ROCK&
gt ;
cofilin&
gt ;
actin pathway also used by
brain - derived neurotrophic factor and
adenosine , and therefore belongs to a family of ' synaptic modulators ' that regulate plasticity . -
pubmed
A calcific
NP biofilm developed within 1 week after inoculation and their development was reduced by
pyrophosphate and inhibitors of
ALP . -
pubmed
Three cases ( 20.0 % ) were refractory to
corticosteroid plus diverse immunosuppressive therapy and were treated with anti -
TNF agents , all of them with disease remission . -
pubmed
The
LPO treatment downregulated levels of reactive
oxygen species in osteoclasts . -
pubmed
Ultimately ,
LPO treatment led to decreased expression of
c - Fos and
NFAT2 . -
pubmed
A rapid turnover of
phosphate pools in the leaves is essential for energy transfer and metabolism within photosynthesis , and consequently , we hypothesized that
PHR1 is needed for adaptation to high - light stress during
P deficiency . -
pubmed
Compared to the wild - type and the overexpressor line , the
phr1 mutant has decreased levels of
phosphate ,
anthocyanins and
carbohydrates during combined P deficiency and light stress . -
pubmed
We conclude that
PHR1 is needed for the metabolic balance , for retaining P(i ) levels and for inducing
anthocyanin production , and during
P deficiency PHR1 is vital for adaptations to avoid permanent damage to
photosystems during high - light conditions . -
pubmed
Endoplasmic reticulum stress decreases intracellular
thyroid hormone activation via an
eIF2a - mediated decrease in
type 2 deiodinase synthesis . -
pubmed
To test whether
D2 is normally processed through
ER - associated degradation ,
ER stress was induced in cells that endogenously express
D2 by exposure to
thapsigargin or
tunicamycin . -
pubmed
ER stress - induced loss of
D2 was prevented in the absence of T(4 ) , by blocking the proteasome with
MG-132 or by treatment with chemical chaperones . -
pubmed
Notably ,
ER stress did not alter
D2 activity half - life but rather decreased
D2 synthesis as assessed by induction of
D2 mRNA and by
[ ( 35 ) S ] methionine labeling . -
pubmed
FITC - labeled GW -
H1 was found to attach to cell membrane initially , then translocated into the cytoplasm , and eventually membranous organelles or nucleus . -
pubmed
INTRODUCTION :
Ticagrelor , an oral
P2Y ( 12 ) receptor antagonist used as part of dual antiplatelet therapy in the treatment of
acute coronary syndromes (
ACS ) , has many favorable characteristics when compared with the more frequently used
P2Y ( 12 ) receptor antagonist
clopidogrel . -
pubmed
Also , the production patterns of these extracellular
enzymes were researched during the growth of the organism for a period of 20 days and the
protein , reducing
sugar , and
nitrogen levels were monitored during the stationary cultivation . -
pubmed
The highest
Lac activity was obtained as 2247.62 + - 75 U / L on day 10 in the presence of 750 microM
Mn2 + , while the highest
MnP activity was attained as 2198.44 + - 65 U / L on day 15 in the presence of 500 microM
Mn2 + . -
pubmed
Kinetic analysis of
iron - dependent
histone demethylases :
alpha - ketoglutarate substrate inhibition and potential relevance to the regulation of
histone demethylation in
cancer cells . -
pubmed
The Jumonji C domain - containing
histone demethylases (
JmjC - HDMs ) are
alpha - ketoglutarate (
alphaKG)-dependent ,
O ( 2 )-activating , non -
heme iron enzymes that play an important role in epigenetics . -
pubmed
A continuous
O ( 2 ) consumption assay reveals that
HDMs have low affinities for
O ( 2 ) , suggesting that these enzymes can act as
oxygen sensors in vivo . -
pubmed
An interesting case of
alphaKG substrate inhibition was found , and the kinetic data suggest that
alphaKG inhibits
JMJD2C competitively with respect to
O ( 2 ) . -
pubmed
JMJD2C displays an optimal activity in vitro at
alphaKG concentrations similar to those found in
cancer cells , with implications for the regulation of
histone demethylation activity in
cancer versus normal cells . -
pubmed
Our aim was to determine the effects of
dexamethasone treatment on wild - type
CFTR expression . -
pubmed
Within 24 h ,
dexamethasone ( 10 nM ) showed a 0.3 - fold decrease in
CFTR mRNA expression , and a 3.2 - fold increase in
alphaENaC mRNA expression compared with control groups . -
pubmed
Dexamethasone ( 10 nM ) induced a 1.97 - fold increase in the
total protein of wild - type
CFTR , confirmed by inhibition by
mifepristone . -
pubmed
To access surface protein expression , biotinylation followed by Western blotting showed that
dexamethasone treatment led to a 2.35 - fold increase in the amount of
CFTR in the cell surface compared with the untreated control groups . -
pubmed
Once protein translation was inhibited with
cycloheximide ,
dexamethasone could not increase the amount of
CFTR protein . -
pubmed
Dexamethasone did not alter the degradation of wild - type
CFTR . -
pubmed
Assessment of the B band of
CFTR within 15 min of metabolic
pulse labeling showed a 1.5 - fold increase in
CFTR protein after treatment with
dexamethasone for 24 h.
Chaperone 90 (
HSP90 ) binding to
CFTR increased 1.55 - fold after treatment with
dexamethasone for 24 h , whereas
chaperone 70 (
HSP70 ) binding decreased 0.30 fold in an immunoprecipitation assay . -
pubmed
CONCLUSION : Mature wild - type
CFTR protein is regulated by
dexamethasone post transcription , involving cotranslational mechanisms with
HSP90 and
HSP70 , which enhances maturation and expression of wild - type
CFTR . -
pubmed
During late larval stages , neural proliferation becomes insensitive to dietary nutrients , and the
steroid hormone
ecdysone acts on
Delta / Notch signaling to promote the switch from symmetric mitoses to asymmetric neurogenic divisions . -
pubmed
Here , we study the role of
oxysterols and their nuclear receptors ,
Liver X Receptor (
LXR ) , in myelinating glial cells of the central and peripheral nervous systems . -
pubmed
We show that the transcripts of Wnt components (
Disheveled ,
TCF3 ,
beta - catenin ) are strongly repressed by
oxysterols in Schwann cells but are activated in oligodendrocytes . -
pubmed
Furthermore , we show by immunofluorescent labeling that
beta - catenin is re - localized on the level of the Golgi apparatus of Schwann cells after incubation with
25 - OH . -
pubmed
Median and mean ion levels were low for unilateral resurfacings (
Cr : median 1.3 microg / l , mean 1.95 microg / l ( & lt ; 0.5 to
16.2 ) ;
Co : median 1.0 microg / l , mean 1.62 microg / l ( & lt ; 0.5 to 17.3 ) ) and bilateral resurfacings (
Cr : median 3.2 microg / l , mean 3.46 microg / l ( & lt ; 0.5 to 10.0 ) ;
Co : median 2.3 microg / l , mean 2.66 microg / l ( & lt ; 0.5 to 9.5 ) ) . -
pubmed
The ratio of
kaempferols to
apigenins of the exudate also shows seasonal variation ( maximum value in summer and minimum in
spring ) . -
pubmed
Galactose modified
trimethyl chitosan -
cysteine (
GTC ) conjugates with various
galactose grafting densities were developed for oral delivery of
Survivin shRNA - expression pDNA ( iSur - pDNA ) and
vascular endothelial growth factor (
VEGF ) siRNA ( siVEGF ) in the synergistic and targeted treatment of
hepatoma . -
pubmed
Dietary
magnesium intake is inversely associated with serum
C - reactive protein levels : meta - analysis and systematic review . -
pubmed
This
post hoc analysis of the Phase III TOWER study evaluated the effects of
teriflunomide treatment on five severe
relapse outcomes :
relapses with sequelae defined by an increase in Expanded Disability Status Scale ( EDSS)/functional system ( FS ) score ( sequelae - EDSS /
FS ) 30 days
post relapse ;
relapses with sequelae defined by the investigator ( sequelae - investigator ) ;
relapses leading to hospitalization ;
relapses treated with intravenous
corticosteroids ; and intense
relapses using the definition of Panitch et al . -
pubmed
Compared with placebo ,
teriflunomide significantly reduced annualized rates of
relapses with sequelae - EDSS /
FS [ 14 mg , 36.6 % ( p = 0.0021 ) ; 7 mg , 31.3 % ( p = 0.0104 ) ] and sequelae - investigator [ 14 mg only , 53.5 % ( p = 0.0004 ) ] , relapses leading to hospitalization [ 14 mg only , 33.6 % ( p = 0.0155 ) ] ,
relapses requiring intravenous
corticosteroids [ 14 mg , 35.7 % ( p = 0.0002 ) ; 7 mg , 21.5 % ( p = 0.0337 ) ] , and intense
relapses [ 14 mg only , 52.5 % ( p = 0.0015 ) ] . -
pubmed
To examine this effect , distortion product otoacoustic emissions ( DPOAEs ) measurements and serum levels of oxidative and antioxidant status [ including
malondialdehyde (
MDA ) ,
superoxide dismutase (
SOD ) ,
catalase (
CAT ) ,
glutathione (
GSH ) ,
glutathione peroxidase (
GPX ) , total oxidant status ( TOS ) , total antioxidant status ( TAS ) , and oxidative stress index ( OSI ) ] were evaluated . -
pubmed
We showed that in cultured microglia , activators of
Nrf2 ( i ) induce antioxidative defense components , ( ii ) reduce
peroxide formation , ( iii ) up - regulate phagocytosis - mediating scavenger receptor
CD36 , and ( iv ) enhance red blood cells ( RBC ) phagocytosis . -
pubmed
Several highly conserved
amino acid residues were found high in
p110 beta ( n = 102 ) . -
pubmed
Subsequently , the number of highly conserved
amino acid restudies was low in
p50alpha and
p55gamma ( n = 15 ) . -
pubmed
This interaction , mainly in groove mode , weakens the
hydrogen bonds between base pairs of the helix inducing denaturation of the double strands , as well as condensation of the macromolecule , from an extended
coil state to a globular state . -
pubmed
A pooled analysis of six randomized placebo - controlled trials ( n=618 ) indicated that responses at 4 weeks may predict response at 6 - 9 weeks , although another pooled analysis of open - label data ( n=338 ) suggests that the probability of a robust response to
atomoxetine [ & gt;=40 % decrease in
ADHD - Rating Scale ( ADHD -
RS ) scores ] may continue to increase beyond 6 - 9 weeks . -
pubmed
We have previously reported that the mechanisms underlying the effects of
caffeine , a non - selective
adenosine A1 / A2 receptor antagonist , combined with
oseltamivir . -
pubmed
These enhancements were inhibited by an
adenosine A2 receptor agonist ,
CGS21680 ( 0.2 mg / kg , subcutaneously ( s.c . ) -
pubmed
Furthermore , an
adenosine A2 receptor antagonist ,
SCH58261 ( 1 and 3 mg / kg , i.p . ) -
pubmed
Our findings suggest an interaction between the central blockade of
adenosine A2 receptors by
caffeine and
OCB - induced behavioral changes . -
pubmed
The results indicated that crosslinked NQC /
PVA hydrogels with
glutaraldehyde (
GA ) are more thermallystable than non crosslinked hydrogels , NQC /
PVA hydrogels swell highly in different buffer solutions as
PVA content increases and the antimicrobial activity of NQC /
PVA hydrogels is higher than NQC itself . -
pubmed
Maternal separation had opposite effects on
NMDA receptor - mediated inhibition of
norepinephrine release in SHR and WKY hippocampus , as it increased inhibition of both
glutamate - stimulated and depolarization - evoked release in SHR hippocampus but not in WKY . -
pubmed
Nucleotide excision repair (
NER ) removes chemically diverse DNA lesions in all domains of life . -
pubmed
These steps are coordinated by
ATP binding and hydrolysis in the ' proximal ' and ' distal '
UvrA ATP - binding sites . -
pubmed
Subsequent
UvrB recruitment requires
ATP hydrolysis in the proximal site . -
pubmed
Among 22 transfer RNAs , 21 have the typical clover - leaf structure , while
tRNASer ( AGN ) does not possess the
dihydrouridine ( DHU ) arm and could not form a stable stem - loop structure . -
pubmed
Inositol features may exert
insulin - like effects . -
pubmed
To determine the type of
cholera toxin subunit B (
ctxB ) ,
nucleotide sequencing was performed . -
pubmed
RESULTS : All the eight
strains , except one
strain , showed resistant to
nalidixic acid ,
sulphamethoxazole ,
streptomycin and
trimethoprim and possessed the
sullI ,
strB ,
dfrA1 and
int SXT genes . -
pubmed
This study tested the hypothesis that prenatal HS may influence
Adenosine - induced vasodilatation via
protein kinase A (
PKA ) pathway in coronary arteries . -
pubmed
Coronary arteries from male adult offspring were tested for
K + channels and
Adenosine signal pathways . -
pubmed
After pretreatment with
PKA inhibitor , vasodilatation to
Adenosine was decreased to a smaller extent in HS than that in control . -
pubmed
Forskolin (
activator of adenylate cyclase)-mediated vasodilatation was decreased in HS . -
pubmed
Iberiotoxin (
large - conductance Ca2 + - activated K + channel [
BK channel ] inhibitor ) attenuated
Forskolin - induced vasodilatation in control , not in HS group . -
pubmed
CONCLUSIONS : Prenatal HS diets altered
Adenosine - mediated coronary artery vasodilatation in the offspring , which was linked to downregulation of
cAMP /
PKA / BK channel pathway . -
pubmed
UV - B Inhibits Leaf Growth through Changes in
Growth Regulating Factors and
Gibberellin Levels . -
pubmed
Interestingly , the decrease in the GZ size correlates with a decrease in the concentration of
GA19 , the immediate precursor of the active
gibberellin ,
GA1 , by UV - B in this zone , which is regulated , at least in part , by the expression of
GRF1 and possibly other
transcription factors of the
GRF family . -
pubmed
Mutations in the beta hairpin of
ribosomal protein uL22 , which is rather distal to the
erythromycin binding site , also generate resistance to the antibiotic . -
pubmed
Hydrogen saline suppresses neuronal cell apoptosis and inhibits the
p38 mitogen - activated protein kinase -
caspase-3 signaling pathway following
cerebral ischemia -
reperfusion injury . -
pubmed
These results demonstrated that
H2 significantly reduced the number of apoptotic cells , and the protein expression of
p38 MAPK and
caspase-3 , compared with the IR group . -
pubmed
Folate plus
zinc supplementation did not show any statistically different effect on serum
testosterone ( P = .86 ) ,
inhibin B ( P = .84 ) ,
FSH ( P = .054 ) , and sperm motility ( P = .169 ) as compared to the placebo . -
pubmed
Potential immunotoxic effects of
trichloroethylene - induced
IV allergic reaction in
renal impairment . -
pubmed
A high degree of
cytokine deposition was observed in the renal tubular proximal epithelial cells in the
TCE - sensitized groups . -
pubmed
The expression levels of genes involved in inflammatory signaling (
TNFAIP2 ,
ACOT7 , and
RORA ) were also associated with
birth weight , suggesting a role for inflammatory processes in
Cd - associated
reproductive toxicity . -
pubmed
All these compounds form hydrophobic interaction , aromatic pi - pi stacking and
hydrogen bond efficiently with the
Eg5 . -
pubmed
The
serotonin transporter polymorphism ( 5 - HTTLPR ) and
cortisol stress responsiveness : preliminary evidence for a modulating role for sleep quality . -
pubmed
The short ( S ) allele of a functional polymorphism ( 5 - HTTLPR ) within the promoter region of the
serotonin transporter gene (
SLC6A4 ) is found to predispose the risk for stress - related
affective disorders relative to the long ( L ) allele . -
pubmed
Accounting for this 5 - HTTLPR - dependent influence enhanced the predictive value of 5 - HTTLPR on
cortisol stress responsiveness , revealing greater
HPA reactivity in S - allele relative to L - allele carriers . -
pubmed
Molecular recognition of
RAS /
RAF complex at the membrane : Role of
RAF cysteine - rich domain . -
pubmed
Activation of
RAF kinase involves the association of its
RAS - binding domain ( RBD ) and
cysteine - rich domain (
CRD ) with membrane - anchored
RAS . -
pubmed
Design , synthesis and antifungal evaluation of novel
pyrazole carboxamides with
diarylamines scaffold as potent
succinate dehydrogenase inhibitors . -
pubmed
And compound 1c ( IC50 = 0.034 mg / L ) showed higher inhibition abilities against
succinate dehydrogenase than
fluxapyroxad ( IC50 = 0.037 mg / L ) . -
pubmed
Chemical immunosuppressive drugs including
mammalian target of rapamycin inhibitors (
sirolimus and
everolimus ) ,
calcineurin inhibitors (
tacrolimus and
cyclosporine ) , and
purine and
pyrimidine synthesis inhibitors (
6 - mercaptopurine ,
mycophenolic acid , and
methotrexate ) are widely prescribed for the treatment of
autoimmune and inflammatory diseases and for controlling alloimmunity in interfering with the signals that activate and allow T cells to proliferate . -
pubmed
We found that
isopsoralen pretreatment significantly reversed the increase in DNA fragmentation and apoptosis rate , and significantly decreased the
caspase-3 activity and
PARP cleavage in
IL-1beta - treated chondrocytes . -
pubmed
Moreover , the expressions of
LC3 - II and
LAMP-1 were markedly increased but the expression of
p62 /
SQSTM1 was remarkably decreased by
isopsoralen pretreatment . -
pubmed
Importantly , the protective effects of
isopsoralen against
IL-1beta were blocked by pretreatment with autophagy inhibitor
3 - MA and
bafilomycin A1 . -
pubmed
Thirty years ago , a novel axis of the
renin -
angiotensin system (
RAS ) was unveiled by the discovery of
angiotensin -( 1 - 7 ) [
ANG -( 1 - 7 ) ] generation in vivo . -
pubmed
Very recently , another branch of the
RAS was discovered , based on
angiotensin peptides in which the
amino - terminal
aspartate was replaced by
alanine , the
alatensins . -
pubmed
Ala - ANG-(1 - 7 ) or
alamandine was shown to interact with
Mas - related G protein - coupled receptor D , and the first functional data indicated that this peptide also exerts protective effects in the cardiovascular system . -
pubmed
ACD incidence decreased by 53 % ( 95%CI : 0.27 - 0.79 ) per 100 cells / L increase in baseline
CD4 - cell count and decreased by 90 % ( 95%CI : 0.01,0.87 ) among adults treated with
nevirapine - containing HAART . -
pubmed
Glucose Oxidase - Based
Glucose - Sensitive Drug Delivery for
Diabetes Treatment . -
pubmed
The
glucose - sensitive drug delivery systems based on
glucose oxidase (
GOD ) , which exhibit highly promising applications in
diabetes therapy , have attracted much more interest in recent years . -
pubmed
The self - regulated drug delivery systems regulate drug release by
glucose concentration automatically and continuously to control the blood
glucose level (
BGL ) in normoglycemic state . -
pubmed
The applications of GOD - immobilized platforms , such as self - assembly layer - by - layer ( LbL ) films and polymer vesicles , cross - linking hydrogels and microgels , hybrid mesoporous
silica nanoparticles , and microdevices fabricated with
insulin reservoirs have been surveyed . -
pubmed
Progression - free survival ( PFS ) superiority with
osimertinib over comparator
EGFR - TKI remained consistent irrespective of randomization route ( central / local EGFRm positive tissue test ) . -
pubmed
In this framework , we report the characterization of
Met - Ageritin , an isoform of
Ageritin with an additional N - terminal
methionyl residue . -
pubmed
Met - Ageritin inhibits protein synthesis in vitro with an IC50 = 2.8 nM that is 21 - fold lower than that of
Ageritin , while not show
endonuclease activity on DNA . -
pubmed
In contrast , reducing
nicotine by reducing number of cigarettes per day (
CPD ) is common . -
pubmed
Participants were then randomized to gradually reduce to 70 % , 35 % , 15 % , and 3 % of baseline
nicotine over 4 weeks by either ( 1 ) transitioning to lower
nicotine VLNC cigarettes or ( 2 ) reducing the number of full
nicotine CPD . -
pubmed
CONCLUSIONS : Reducing number of
CPD increased QAs more than reducing
nicotine via switching to VLNC cigarettes . -
pubmed
IMPLICATIONS : Reducing the frequency of smoking behavior ( ie ,
CPD ) could be a more effective strategy to increase QAs than reducing the magnitude of
nicotine in each cigarette ( ie , VLNC ) per se . -
pubmed
The
MoS2@
polypyrrole@
Fe3O4 composite was skillfully synthesized via a novel one
pot method . -
pubmed
In addition , the one
pot method used in this work can promisingly be applied to prepare other similar composites such as
carbon fiber@
polypyrrole@
Fe3O4 ,
graphene@
polypyrrole@
Fe3O4 . -
pubmed
The liver is enriched with polyunsaturated
triglycerides (
PUFA -
TGs ) in
PNPLA3 - I148 M carriers . -
pubmed
RESULTS :
PUFA - TGs were significantly increased in
AT in carriers versus non - carriers of
PNPLA3 - I148M. -
pubmed
PNPLA3 - I148 M locally remodels AT TGs to become polyunsaturated as it does in the liver , without affecting lipolysis or composition of serum
NEFAs . -
pubmed
We measured
iPTH ,
oxPTH and
n - oxPTH at study entry in 600 kidney transplant recipients ( KTRs ) . -
pubmed
Length of root and hypocotyl was enhanced by BS compared to
CS silver NPs . -
pubmed
From proteomics , abundances of
peroxidases were increased under
CS silver NPs . -
pubmed
Immunoblot analysis depicted that accumulation of
ascorbate peroxidase ,
glutathione reductase , and
peroxiredoxin remained unchanged under both
BS and
CS silver NPs . -
pubmed
ATP content decreased under
CS silver NPs compared to BS
silver NPs . -
pubmed
Length of root and hypocotyl was enhanced by
GS silver NPs compared to
CS silver NPs . -
pubmed
From the proteomics , the abundances of
peroxidases were increased under
CS silver NPs . -
pubmed
Immunoblot analysis depicted that accumulation of
ascorbate peroxidase ,
glutathione reductase , and
peroxiredoxin remained unchanged under both
GS and
CS silver NPs . -
pubmed
ATP content decreased under
CS silver NPs compared to
GS silver NPs . -
pubmed
The 26S proteasome is a 2.5 - MDa
protease complex responsible for the selective and
ATP - dependent degradation of ubiquitylated proteins in eukaryotic cells . -
pubmed
Accompanied by deciphering the structural diversity of ubiquitin chains with eight linkages and chain lengths , the
ubiquitin code for proteasomal degradation has been expanding beyond the best - characterized
Lys48 - linked ubiquitin chains . -
pubmed
One of the latter ( rs13266634 ) locates in a
SLC30A8 exon , encoding a
tryptophan - to -
arginine substitution that decreases
SLC30A8 function , being the canonical explanation for
type 2 diabetes risk association . -
pubmed
METHODS : This multicenter prospective study involved 25 cases of
fungal keratitis that were treated by DALK with cross - linked APCSs and
post - operative topical
tacrolimus from December 2013 to November 2014 at the Wenzhou Eye Hospital and the Henan provincial Eye Hospital . -
pubmed
Post - operative topical
tacrolimus alleviated the ciliary injection , except in one case which acute stromal rejection occurred . -
pubmed
CONCLUSIONS : DALK using cross - linked
APCS combining topical
tacrolimus treatment is safe and effective in managing
fungal keratitis . -
pubmed
Tauroursodeoxycholic acid (
TUDCA ) , an inhibitor of ERS , or vehicle was administered intracerebroventricularly for 4 weeks
post - HF induction . -
pubmed
Analysis of rostral ventrolateral medulla gene expression confirmed the presence of ERS ,
inflammation , and activation of
renin -
angiotensin system pathways in high output
HF and showed that
TUDCA treatment completely abolished ERS and ERS - related signaling . -
pubmed
Response to comments on : Short - term outcome of
botulinum neurotoxin A injection with or without
sodium hyaluronate in the treatment of
infantile esotropia - A prospective interventional study . -
pubmed
Regulation of
valproic acid induced
EMT by
AKT /
GSK3beta /
beta - catenin signaling pathway in triple negative
breast cancer . -
pubmed
Valproic acid (
VPA ) is a selective histone deacetylation (
HDAC ) inhibitor and exerts anti -
cancer properties in different types of
cancer . -
pubmed
Therefore , we aimed to clarified the unravel relationship between
AKT /
GSK3beta /
beta - catenin signalling pathway and
VPA - induced EMT in triple negative
breast cancer (
TNBC ) . -
pubmed
Our results showed that
VPA remarkably inhibited the growth of
TNBC cell and triggered apoptotic cell
death through G0 /
G1 arrest . -
pubmed
Furthermore ,
VPA increased cell migration and activated the
EMT process through significantly increasing
Snail expression and in turn downregulation of
E - cadherin and
GKS3beta levels . -
pubmed
However , the level of
AKT and
beta - catenin was reduced after treatment of
VPA . -
pubmed
Our data showed that
VPA induced
EMT process and cell migration in
TNBC cells . -
pubmed
In Situ Exosomal MicroRNA Determination by Target - Triggered
SERS and
Fe3O4@
TiO2 - Based Exosome Accumulation . -
pubmed
The most common adverse events were
fatigue ( 12.6 % ) ,
pruritis ( 7.3 % ) , increased serum
ALT ( 4.7 % ) and
bilirubin ( 3.8 % ) levels , and
anemia ( 3.1 % ) . -
pubmed
This contrast allows accurate identification of fluorescent
CSF cells and structures in
formalin fixed paraffin embedded brain tissues ; in the latter , the combined optical procedure is fruitfull in order to avoid fluorescent artifacts misinterpretation . -
pubmed
NT and NE caused a decrease in serum
cholesterol and phospholipid levels , an increase in liver
cholesterol level , no significant change in
triglyceride levels of both serum and liver , with a significant change in serum lipoprotein patterns ; a decreased in
alpha - and beta - lipoproteins and a marked increase in pre beta - lipoprotein . -
pubmed
Pure
NT decreased serum
cholesterol without causing any change in lipoprotein pattern . -
pubmed
MAP ,
CMA and
DOCA causee almost no effect on lipid levels in serum and liver , but
CMA and
DOCA increased
alpha - lipoprotein and decreased
beta - and pre beta - lipoproteins . -
pubmed
An acute treatment with
estradiol caused a decrease in
alpha - and beta - lipoproteins and an increase in pre beta - lipoprotein with a decrease in serum lipid levels and an increase in liver lipids . -
pubmed
Transamination rates of
3 - hydroxy - DL - kynurenine and
5 - hydroxy - DL - kynurenine by the
enzyme were 1/2.9 and 1/2.6 of
kynurenine . -
pubmed
The
enzyme showed a higher preference for
pyruvate than
2 - oxoglutarate as aminoacceptor . -
pubmed
Besides high resistances to
adriamycin ,
actinomycin D , and
vincristine , we observed a lowered sensitivity towards
bleomycin , a rather hydrophilic drug usually not involved in
P - glycoprotein associated MDR . -
pubmed
The expression of this virus is regulated by
mammary cell type - specific factors ,
steroid hormones , and polypeptide
growth factors . -
pubmed
Besides
ketanserin , the descending inhibition was blocked by
ritanserin ,
haloperidol , and
pipamperone , which have affinities to
5 - hydroxytryptamine2 receptors , and also by
spiperone and
methiothepin , which are antagonists at both
5 - hydroxytryptamine1 and
5 - hydroxytryptamine2 receptors ( all 1 microM ) . -
pubmed
The results with these antagonists favor the involvement of
5 - hydroxytryptamine2 receptors although the results with
mesulergine disagree with this notion . -
pubmed
5 - Hydroxytryptamine1 agonists , such as
8 - hydroxy-2 -( di - n - propylamino ) tetralin ,
buspirone , and
5 - carboxyamidotryptamine , and a
5 - hydroxytryptamine3 agonist ,
2 - methyl-5 - hydroxytryptamine , induced a long - lasting inhibition of the monosynaptic reflex , which was blocked by
ketanserin whereas a
5 - hydroxytryptamine2 agonist ,
S -(+)- alpha - methyl-5 - hydroxytryptamine , evoked a biphasic inhibition , in which only the later component was blocked by
ketanserin.(ABSTRACT TRUNCATED
AT 400 WORDS ) -
pubmed
Oncoprotein - mediated signalling cascade stimulates
c - Jun activity by phosphorylation of
serines 63 and 73 . -
pubmed
Phosphorylation of these residues ,
serines 63 and 73 , stimulates the transactivation function of
c - Jun and is required for oncogenic cooperation with
Ha - Ras . -
pubmed
These oncoproteins ,
v - Sis ,
v - Src ,
Ha - Ras , and
Raf-1 , participate in a signal transduction pathway that leads to increased phosphorylation of
serines 63 and 73 on
c - Jun. While oncogenic
Ha - Ras is a constitutive stimulator of
c - Jun activity and phosphorylation , the normal
c - Ha - Ras protein is a serum - dependent modulator of
c - Jun ' s activity . -
pubmed
Support for the segregation of
NMDA and non - NMDA receptors in discrete anatomical patches arises from the observation that the fast and slow components of dual - component potentials mediated via
NMDA and non -
NMDA receptor types can fail independently . -
pubmed
We report the occurrence of spontaneous
TTX - resistant EPSPs in sensory interneurons that are mediated by
EAA receptors . -
pubmed
These data suggest that spontaneous EPSPs reflect release of
EAA neurotransmitter at synaptic contacts overlying homogeneous populations of either
NMDA or non - NMDA receptors . -
pubmed
Evaluation of a multicentre study with
Iomazenil -- a
benzodiazepine receptor ligand . -
pubmed
After showing in an earlier publication that
Iomazenil is a potent
benzodiazepine receptor antagonist , the substance has been distributed to 11 clinical centres in Europe for further tests . -
pubmed
Prior to the
Iomazenil examination , flow images by
perfusamine or
HMPAO were required , and as comparative methods EEG , computed tomography (
CT ) and magnetic resonance imaging ( MRI ) were performed . -
pubmed
In vitro studies indicate that in the absence of
sodium chloride , compounds 3a , 4a ,
N - propyl - N - norbuprenorphine ( 5 ) ,
buprenorphine and
diprenorphine are reasonably comparable in binding affinity for
opioid receptors . -
pubmed
The endothelium - derived relaxing factor generated by
bradykinin in this vascular bed is an
oxygen radical generated in association with enhanced
arachidonate metabolism via
cyclooxygenase . -
pubmed
Following intraarterial ( IA ) or intravenous (
IV ) administration of
cisplatin ( 2.5 mg / kg ) , changes in the
tumor cells were examined electron microscopically . -
pubmed
Assessments were made of
sinus node function , intra - atrial , atrioventricular ( AV )
nodal and His - Purkinje conduction , and atrial and ventricular refractoriness in the control state and during RVS and LVS at 4 - 12 Hz . -
pubmed
Long term haemodynamic ,
pulmonary function and symptomatic effects of
pirbuterol in
COPD . -
pubmed
In the absence of UV irradiation ,
m - ATIP inhibited K+ -stimulated
ATPase activity in lyophilized gastric vesicles competitively with respect to K+ , with a Ki value of 2.4 microM at pH 7.0 . -
pubmed
Irradiation of lyophilized gastric vesicles at pH 7.0 with
[ 14C ] m - ATIP in the presence of 0.2 mM
ATP resulted in a time - dependent inactivation of
ATPase activity that was associated with an incorporation of radioactivity into a 100 - kDa polypeptide representing the catalytic subunit of the
( H + + K +)- ATPase . -
pubmed
A mechanism by which
nitrate receptors could be manipulated to increase vascular responsiveness is theorized , as well as a means by which a
nitrate - free interval might avoid the development of hemodynamic attenuation in terms of cellular mechanisms and receptors . -
pubmed
Mitochondrial
succinate dehydrogenase activity was decreased in the presence of
MEHP . -
pubmed
Pulmonary function was evaluated by means of spirometry and a single breath
nitrogen washout . -
pubmed
Following the introduction of
chlormethiazole , there was a significant reduction in
DT mortality and in duration of
DTs . -
pubmed
Glucose and
insulin levels in obese non - diabetics . -
pubmed
[ Two - dimensional electrophoresis of the products of limited hydrolysis of
human serum albumin using isoelectric focusing in a
borate -
polyol system ] . -
pubmed
A procedure developed involved isoelectrofocusing in
borate -
polyol system and thin - layer electrophoresis in
polyacrylamide gel gradient containing
sodium dodecylsulfate of the products of
human blood serum albumin obtained after partial hydrolysis . -
pubmed
Mean serum total
cholesterol concentration was 5.7 mmol / l ; about 60 % of this
cholesterol was located in the
HDL fraction ( density greater than 1.063 g / ml ) . -
pubmed
The
LDL ( defined as heparin -
manganese precipitable lipoproteins in
VLDL and
chylomicron - deficient serum ) contained 49 % of
cholesterol and 43 % of phospholipids ( on the basis of total
LDL lipids ) . -
pubmed
The
HDL ( defined as heparin -
manganese soluble lipoproteins in
VLDL and
chylomicron - deficient serum ) contained 36 % of
cholesterol and 58 % of phospholipids ( on the basis of total
HDL lipids ) . -
pubmed
[ Conformational changes in ionic pump structure during interaction of
Na plus - K plus - ATPase with
ouabain ] . -
pubmed
[ Diffusion procedures for the determination of
ammonia within the framework of the
post mortem diagnosis of
uremia ] . -
pubmed
[
Actinomycin D derivatives that recognize 2 DNA
GC pairs :
bis - actinomycin D ] . -
pubmed
In both
WR and
WD subjects selective unloading of cardiac baroreceptors with lower body negative pressure ( LBNP ) -15 mmHg for 30 min did not increase
AVP levels . -
pubmed
Further analysis of the data based on serum osmolalities (
OSM ) indicated that LBNP -40 mmHg significantly increased
AVP levels ( 3.72 + /- 0.43 to 13.59 + /- 3.63 microU / ml ; P less than 0.05 ; n = 9 ) during sessions with
OSM greater than 294 mosmol / kg , but not during sessions with
OSM less than 294 mosmol / kg ( 2.85 + /- 0.29 to 4.66 + /- 1.15 microU / ml ; P =
NS ; n = 11 ) . -
pubmed
Gas chromatography of the acid - hydrolyzed protein confirmed the presence of
fatty acid covalently associated with the
protein presumably through an
amino linkage . -
pubmed
Digestion of the
fatty acid - containing peptide with
pronase resulted in the
fatty acid together with
L - alanine becoming extractable into
hexane . -
pubmed
Overall , the prevalence of autoantibodies against
thyroid peroxidase ,
thyroglobulin ,
thyroxine , and
triiodothyronine was 45 % , 18 % , 42 % , and 36 % , respectively . -
pubmed
Two of these are in the helix 1A subdomain of the
type II keratin 1 , giving a serine - to - proline substitution in codon 185 and an asparagine - to -
serine substitution in codon 187 . -
pubmed
In the analogous region of
type I keratin 10 , an arginine - to - proline and an arginine - to -
serine transition in codon 156 have been identified . -
pubmed
Intracerebroventricular injection of
capsaicin and SP antiserum had no effect on the immune response to
SRBC . -
pubmed
Treatment with
retinoic acid ( RA , 100 nM ) increased medium concentrations of
IGFBP-3 to 175 + /- 8 % ( mean + /-
SE , n = 4 ) , and
IGFBP-6 to 217 + /- 20 % of control values . -
pubmed
In the presence of 100 nM
RA , the stimulation elicited by these agents was enhanced , with
IGFBP-3 levels increasing to 6 - fold above that seen with
RA alone . -
pubmed
IGFBP-6 increased 12 - fold with
RA +
forskolin and 20 - fold with RA +
dbcAMP . -
pubmed
Estrogen ( 10 nM
estradiol ) reduced basal
IGFBP-3 levels by 25 % but increased
IGFBP-6 1.5- to 2 - fold . -
pubmed
The stimulatory effect of RA +
forskolin on
IGFBP-3 was partially reversed by
estrogen , whereas RA +
forskolin - stimulated
IGFBP-6 levels were further increased by
estrogen . -
pubmed
Increased
IGFBP-3 and - 6 production in response to RA +
forskolin was accompanied by a decrease in
IGF - stimulated
thymidine incorporation into DNA ; by contrast , the bioactivity of an
IGF analog that does not bind with
IGFBPs , [
Gln3 , Ala4 ,
Tyr15 , Leu16]
IGF - I , was unchanged under these conditions . -
pubmed
Further ,
IGFBP-3 and
IGFBP-6 are differentially regulated by
estrogen . -
pubmed
However , all of these studies have been limited to the use of exogenous
reagents to perturb
GalTase function . -
pubmed
Tn and
sialyl - Tn carbohydrate structures , first identified in
glycophorins of persons with the rare
Tn syndrome , were found to be present on the surface of most
cancer cells . -
pubmed
In this article , the studies on
Tn and
sialyl - Tn antigens as diagnostic and prognostic
tumor markers and as immunogens in vaccines for
cancer immunotherapy are shortly reviewed . -
pubmed
Biochemically , there was increase of
AST ,
ALT and
cholesterol in 48 % , of
GGT in 52 % and of
alkaline phosphatase in 38 % . -
pubmed
The effects of free radicals on voltage - gated
Ca2 + currents ( ICa ) were investigated in single guinea - pig ventricular myocytes using the whole - cell
clamp technique . -
pubmed
ICa was measured in the baseline state and after the application of free radicals from
cumene hydroperoxide or generated from the addition of
purine to
xanthine oxidase . -
pubmed
ICa also decreased from 1303 + /- 560 pA to 965 + /- 360 pA in the presence of the free radical generating system ( 2.3 mM
purine plus 20 U / l
xanthine oxidase ) . -
pubmed
The administration of
CaNa2EDTA or
DMSA was more effective than that of
DMPS , and the combination of
CaNa2EDTA and
DMSA was more efficient than that of
CaNa2EDTA and
DMPS or the individual chelators in enhancing urinary / fecal excretion of lead , in reducing hepatic , renal , and femur concentration of lead , and in restoring lead - induced inhibition of
blood delta - aminolevulinic acid dehydratase and increase in blood
zinc protoporphyrin and urinary
delta - aminolevulinic acid levels . -
pubmed
We now found that
CK II is activated only when the cells are exposed to both
IGF-1 and
TF or when
TF is replaced in this combination with relatively high levels of
iron salts . -
pubmed
Using cells either wild type for
acetyl phosphate metabolism or defective for
phosphotransacetylase or
acetate kinase , or both , we measured flagellar expression and the intracellular concentration of
acetyl phosphate relative to growth phase and temperature . -
pubmed
To extend these observations , we measured the intracellular concentration of
acetyl - CoA , the level of expression from the
pta and
ackA promoters , and the activities of
phosphotransacetylase and
acetate kinase derived from cell lysates . -
pubmed
Relative to increasing culture density ,
acetyl - CoA levels and expression from both the
pta and
ackA promoters decreased . -
pubmed
We propose that cells regulate intracellular
acetyl phosphate concentrations relative to growth phase and temperature by modulating the availability of
acetyl - CoA , the expression of
ackA , and the activity of
phosphotransacetylase . -
pubmed
This selective activation suggests that the mechanism of action of
pentylenetetrazol , as a convulsant , is not simply as an antagonist of
GABA receptors . -
pubmed
METHODS : Renal function was assessed by
S - creatinine and the
albumin /
creatinine clearance ratio , and arthritic activity by joint score and
C - reactive protein (
CRP ) . -
pubmed
Prompt institution of
corticosteroid treatment is regarded as indispensable at increase of
CRP / S - AA due to
infection or surgery . -
pubmed
Our data suggest a role for immunoregulatory
cytokines and
prostanoids such as
IL-1 beta and
PGE2 in these interactions . -
pubmed
Carboxy terminal sequence and synthesis of
rat kidney laminin gamma 1 chain . -
pubmed
N - Ethylmaleimide inhibited alternative
oxidase activity , but
iodoacetate was found to stimulate activity much like
pyruvate , including enhancing the enzyme 's apparent affinity for reduced
ubiquinone . -
pubmed
The presence of
pyruvate or
iodoacetate blocked inhibition of the
enzyme by
N - ethylmaleimide , indicating that all three compounds acted at the same
sulfhydryl group on the alternative
oxidase protein . -
pubmed
The site of
pyruvate and
iodoacetate action was shown to be a different
sulfhydryl than that involved in the redox - active regulatory
disulfide bond , because
iodoacetate bound to the alternative
oxidase at the activating site even when the redox - active regulatory
sulfhydryls were oxidized . -
pubmed
Given the nature of the covalent adduct formed by the reaction of
iodoacetate with
sulfhydryls , the activation of the alternative
oxidase by
alpha - keto acids appears to involve the formation of a
thiohemiacetal . -
pubmed
Dihydrobenzophenanthridine ( DHBP ) oxidase catalyses the last step in the biogenesis of the
benzo [ c ] phenanthridine alkaloid sanguinarine . -
pubmed
Enzyme - specific activity could be induced in a dose- and time - dependent manner up to 4- to 14 - fold , respectively , when cells were treated with
MeJ or with
ASA . -
pubmed
canadensis defence against pathogens by eliciting the
enzymes involved in the synthesis of the
phytoalexin benzophenanthridine alkaloids . -
pubmed
Castasterone ,
6 - deoxocastasterone ,
typhasterol and
6 - deoxotyphasterol were identified by
GC -
MS . -
pubmed
The
45Ca2 + release was not inhibited by
heparin , an antagonist of
IP3 receptor . -
pubmed
The effects of
caffeine ,
ryanodine and
cADPR on
45Ca2 + release were also tested in the presence of
thapsigargin (
TG ) , an inhibitor of microsomal
Ca2 + - ATPase . -
pubmed
The properties of the
ryanodine - sensitive mechanism are similar to that of the
ryanodine receptor as described in muscle cells . -
pubmed
Two of the 16 subjects had familial
ALS and mutations in the gene for
Cu /
Zn SOD1 . -
pubmed
Using
3H - daunomycin (
3H - DM ) and
calcein as
MRP substrates and
probenecid (
PRO ) ,
genistein (
GEN ) ,
benzbromarone ( BB ) ,
N - ethylmaleimide (
NEM ) and
verapamil (
VP ) as
MRP modulators , drug accumulation studies revealed a transporting activity of
MRP that correlated significantly with the gene expression data . -
pubmed
Moreover , a significant correlation between
MRP expression and chemoresistance against
daunomycin ( DM ) ,
doxorubicin (
DOX ) ,
etoposide (
VP-16 ) and
vinblastine (
VBL ) , but not
cisplatin (
CDDP ) and
bleomycin (
Bleo ) (
MTT - based survival assay ) , was detected . -
pubmed
Synthesis and radiolabeling of
( S )- 4 - amino-5 - iodo-2 - methoxy - N -( 1 - azabicyclo [ 2.2.2 ] oct-3 - yl ) benzamide , the active enantiomer of
[ 125I ] iodozacopride , and re - evaluation of its
5 - HT3 receptor affinity . -
pubmed
We report an improved synthesis of unlabeled
( S )- iodozacopride , the radiolabeling of
( S )-[ 125I ] iodozacopride via
deschloro -( S )- zacopride , and a re - evaluation of its affinity for the
5 - HT3 receptor . -
pubmed
The cDNA encoding the 86 kDa
arginine kinase was amplified by PCR and the cDNA - derived
amino acid sequence of 724 residues was determined . -
pubmed
The
amino acid sequence clearly indicates that Pseudocardium
arginine kinase has a two - domain structure : the first domain residues 1 - 363 and the second domain 364 - 724 . -
pubmed
Comparison of the
amino acid sequence in the
GS region ( a possible candidate for the
guanidine substrate recognition site in the
phosphagen kinase family ) suggests that the first domain of
Pseudocardium arginine kinase might not retain a complete enzyme activity , because the
Asp-7 in the
GS region , which is assumed to be involved in the recognition of the positive charge of
arginine , was replaced by a
Gly residue in the first domain . -
pubmed
Effects of extracellular
acetylcholine on
muscarinic receptor binding assessed by
[ 125I ] dexetimide and a simple probe . -
pubmed
[ 125I ] Dexetimide reached a plateau at 90 min
post - injection . -
pubmed
After the protein was isolated and the
N - terminal amino acid sequence determined , a protein database analysis revealed that it is
high mobility group-1 ( HMG-1 ) protein . -
pubmed
The interactions with the transition - state analogues are , in general , very similar to those seen previously for the complex of the
enzyme with a
bestatin - based inhibitor . -
pubmed
These observations tend to support one of the mechanistic schemes for
eMetAP considered before , although with a revision in the role played by
His79 . -
pubmed
Since a sub - population of
NPY - producing neurons in the arcuate nucleus (
ARC ) of the hypothalamus co - express
GABA , the possibility of an interplay between
NPY and
GABA in the release of
LH was investigated in two ways . -
pubmed
These morphological findings raised the possibility that inhibitory
GABA co - released with
NPY may act to restrain the excitatory effects of
NPY on
LH release . -
pubmed
On the other hand , administration of
MUS blocked the
NPY - induced stimulation of
LH release in a dose - dependent manner . -
pubmed
Similarly , administration of
MUS abolished the excitatory effects on
LH release of
1229U91 , a selective
NPY Y4 receptor agonist . -
pubmed
These results support the possibility that in the event of co - release of these neurotransmitters / neuromodulators ,
GABA may act to restrain stimulation of
LH release by
NPY during the basal episodic and cyclic release of
LH in vivo . -
pubmed
[ Changes in synthesis of
nitric oxide , blood levels of
ACTH and
cortisol in
viral hepatitis B ] . -
pubmed
ACTH and
hydrocortisone blood levels were significantly high in AVH - B and in CH - B exacerbation . -
pubmed
A negative correlation between
nitrituria /
nitraturia and
ALAT ,
ASAT ,
bilirubin ,
alkaline phosphatase ,
gamma - glutamyl - transpeptidase is an indirect evidence for a protective role of
NO against
viral hepatitis B. -
pubmed
The performance of
silica gel based cation - exchangers such as BioSil
CAT is in most cases better than observed for sulfoacylated resins . -
pubmed
The
enzyme activity of
CYP2C9 catalyzing oxidation of
tolbutamide to
hydroxy tolbutamide in S9 fraction of the cell was determined by high performance liquid chromatography(HPLC ) . -
pubmed
RESULTS : The
amino acid sequence predicted from the cDNA segment was identical to that of
CYP2C9 * 1 , the wild type
CYP2C9 . -
pubmed
21T>C , 1146C>T , but the encoding
amino acid sequence was the same ,
L7 , P382 . -
pubmed
One hundred and eighty - five conventional
fatty acids were identified by
GC -
MS , out of which five were new
multibranched polyunsaturated fatty acids . -
pubmed
The
3 - hydroxy-3 - methylglutaryl ( HMG )- CoA reductase inhibitors or statins are potent inhibitors of
cholesterol synthesis . -
pubmed
Microarray - based analysis of anti - angiogenic activity of
demethoxycurcumin on human umbilical vein endothelial cells : crucial involvement of the down - regulation of
matrix metalloproteinase . -
pubmed
Left hemihepatectomy (
LHX ) was performed without in situ perfusion ( control - LHX , n = 5 ) or with concomitant in situ perfusion with hypothermic ( 4 degrees C ) Ringer -
glucose ( cold - LHX , n = 5 ) or normothermic ( 38 degrees C ) Ringer -
glucose ( warm -
LHX , n = 5 ) . -
pubmed
Permeabilization in a cerebral endothelial barrier model by
pertussis toxin involves the
PKC effector pathway and is abolished by elevated levels of
cAMP . -
pubmed
Cellular intoxication by
pertussis toxin proceeds via
ADP - ribosylation of
alpha - G ( i ) proteins , which not only interferes with the homeostatic inhibitory regulation of
adenylate cyclase stimulation but also results in a modulation of the membrane receptor coupling . -
pubmed
Increasing intra - endothelial
cAMP levels by employing
cholera toxin or
forskolin even inhibited the
pertussis toxin - induced permeabilization of endothelial barriers . -
pubmed
In summary , we conclude that permeabilization of cerebral endothelial monolayers by
pertussis toxin does not depend on elevated
cAMP levels and proceeds via the
phosphokinase C pathway . -
pubmed
However , the BIS values on awakening were highly variable , suggesting that it reflects both the residual depressant effects of
methohexital and
post - ictal
depression . -
pubmed
IMPLICATIONS : The bispectral index ( BIS ) value immediately before the electroconvulsive therapy (
ECT ) stimulus correlates with the duration of the motor and electroencephalogram ( EEG )
seizure activity during
methohexital anesthesia . -
pubmed
[ Effect of cardiac
glycosides on
actomyosin . - pubmed
[ SPECTROPHOTOMETRIC METHOD FOR THE DETECTION OF
NITRIC ACID AND
HYDROCHLORIC ACID IN THE PRESENCE OF
NITRATES AND
CHLORIDES IN THE
AIR ] . -
pubmed
ACCELERATING EFFECT OF
COPPER ION ON THE REACTIVATION OF REDUCED
TAKA - AMYLASE A THROUGH CATALYSIS OF THE OXIDATION OF
SULFHYDRYL GROUPS . -
pubmed
[ TREATMENT OF
CORONARY CIRCULATORY DISORDERS WITH
ISOPTIN IN GENERAL PRACTICE ] . -
pubmed
Lifestyle changes as recommended in
diabetes are fundamental for treatment ; addition of
insulin - sensitising agents ( eg ,
metformin ) may be valuable in circumstances such as
anovulatory infertility . -
pubmed
Infertility can be treated successfully in most women by diet and exercise ,
clomiphene citrate with or without
metformin , ovarian drilling , or ovulation induction with
gonadotrophins ; in - vitro fertilisation should be avoided unless there are other indications . -
pubmed
Dependence of
lipoprotein - lipase - catalyzed
triacylglycerol hydrolysis on droplet size of synthetic monodisperse emulsions measured with static light scattering . -
pubmed
These emulsions were used as synthetic substrates for
lipoprotein lipase , a key enzyme for the hydrolysis of serum
triacylglycerols . -
pubmed
Our prior studies demonstrated that peri - sciatic zymosan activated macrophages and neutrophils to release proinflammatory
cytokines and reactive
oxygen species ( ROS ) . -
pubmed
A skin prick test ,
pulmonary function test ,
histamine challenge test and exercise challenge test (
ECT ) were performed in all subjects . -
pubmed
Polymorphisms in
ABCG5 and
ABCG8 transporters and plasma
cholesterol levels . -
pubmed
ABCG5 and
ABCG8 transporters play an important role in the absorption and excretion of
sterols . -
pubmed
The Tyr54Cys polymorphism influenced the degree of reduction in total plasma
cholesterol ( delta -0.49 mmol / l in
Tyr54 homozygotes vs. delta +0.12 mmol / l in
Cys54 homozygotes , p<0.04 ) and
LDL -
cholesterol ( delta -0.57 mmol / l in Tyr54 homozygotes vs. delta +0.04 mmol / l in
Cys54 homozygotes , p<0.03 ) levels between 1988 and 1996 in females , but not in males . -
pubmed
Male Thr400 homozygotes exhibited a greater decrease in total
cholesterol ( delta -0.90 mmol / l vs. delta -0.30 mmol / l , p<0.02 ) and
LDL -
cholesterol ( delta -0.62 mmol / l vs. delta -0.19 mmol / l , p<0.04 ) than Lys400 carriers . -
pubmed
We conclude that Tyr54Cys and Thr400Lys variations in the
ABCG8 gene may play a role in the genetic determination of plasma
cholesterol levels and could possibly influence the gender - specific response of plasma
cholesterol levels after dietary changes . -
pubmed
TRPM8 is a TRP family cation channel which can be activated by cold stimuli or
l - menthol . -
pubmed
[ A new treatment of
keratectasia after LASIK by using
collagen with
riboflavin / UVA light cross - linking ] . -
pubmed
This mutation in the conserved cytoskeleton - associated protein ,
glycine - rich (
CAP - Gly ) domain lowers the affinity of
p150Glued for microtubules and
EB1 . -
pubmed
Cell lines from patients are morphologically normal but show delayed recovery after
nocodazole treatment , consistent with a subtle disruption of
dynein /
dynactin function . -
pubmed
In summary , the energy - metabolism oscillation is basically regulated by a feedback loop of
oxido - reductive reactions of energy metabolism mediated by metabolites like
NADH and
ATP , and is modulated by metabolism of storage
carbohydrates in combination of
post - translational and transcriptional regulation of the related enzymes . -
pubmed
Here I propose a model in which an alteration in
oxygen metabolism is the key cellular event involved in
HIF-1 activation under hypoxic and nonhypoxic conditions . -
pubmed
Experimental studies have suggested that
angiotensin converting enzyme inhibitors and
calcium channel blockers may prevent or arrest progression of
renal injury . -
pubmed
The positron emission tomography with
F18 17beta - estradiol has the potential to benefit diagnosis and treatment of
endometrial cancer . -
pubmed
BACKGROUND : The positron emission tomography ( PET ) with
F18 17beta - estradiol (
FES ) has good imaging for assessment of
estrogen receptor in
breast cancer . -
pubmed
Before hormone treatment was started ,
FES - PET showed increased uptake of endometrium , magnetic resonance imaging ( MRI ) showed thickness and
F-18 fluorodeoxyglucose (
FDG)-PET showed increased uptake . -
pubmed
FES - PET after 3 months showed remaining
FES uptake , but there were no abnormal findings on MRI and
FDG - PET . -
pubmed
CONCLUSIONS : To our knowledge , this is the first report that
FES - PET has the potential to provide more useful information than did
FDG - PET about the hormone therapy . -
pubmed
Oxygen sensing and redox signaling : the role of
thioredoxin in embryonic development and
cardiac diseases . -
pubmed
Here we discuss the roles of
TRX on
oxygen stress and redox regulation from different perspectives , in embryogenesis and in
adult diseases focusing on
cardiac disorders . -
pubmed
Interventional radiology ( IR ) comprising thrombolysis and percutaneous transluminal angioplasty (
PTA ) is well established in the management of thrombosed
polytetrafluoroethylene (
PTFE ) grafts . -
pubmed
The
PKC inhibitor
GF-109203x , the
MEK inhibitor
PD-98059 , and the
p38 MAPK inhibitor
SB-203580 significantly reduced this contraction . -
pubmed
These inhibitory effects were abolished when the
microcystin concentration was increased to 10 muM , indicating that application of these
kinase inhibitors generated an increase in
MLCP activity . -
pubmed
On the other hand ,
SB-203580 , but not
GF-109203x or
PD-98059 , significantly reduced the phosphorylation level of the
PKC - potentiated
phosphatase inhibitor of 17 kDa (
CPI-17 ) . -
pubmed
A
zipper - interacting protein kinase (
ZIPK ) inhibitor (
SM1 peptide ) and a
Rho - associated kinase inhibitor (
Y-27632 ) had little effect on
microcystin - induced contraction at pCa 9 . -
pubmed
In conclusion ,
PKC ,
ERK1 / 2 , and
p38 MAPK pathways facilitate
microcystin - induced contraction at low
Ca2 + concentrations by contributing to the inhibition of
MLCP activity either through phosphorylation of
MYPT1 or
CPI-17 [ probably mediated by
integrin - linked kinase (
ILK ) ] . -
pubmed
The negative regulation of
MLCP by
PKC and
MAPKs during
microcystin - induced contraction at pCa 9 , which is not observed in vascular smooth muscle , may be unique to phasic smooth muscle . -
pubmed
All
NLA derivatives strongly inhibited relaxation of supercoiled DNA catalyzed by either
Topo I or II , in a concentration - dependent manner , without stabilization of a cleavable complex . -
pubmed
In this study , we examined the effects of
Rem , a member of the RGK (
Rem ,
Rem2 ,
Rad ,
Gem /
Kir ) family of
Ras - related monomeric
GTP - binding proteins , on the function of the skeletal muscle
L - type Ca ( 2 + ) channel . -
pubmed
EC coupling was found to be weakened in myotubes expressing
Rem tagged with enhanced
yellow fluorescent protein ( YFP -
Rem ) , as assayed by electrically evoked contractions and myoplasmic
Ca ( 2 + ) transients . -
pubmed
This impaired EC coupling was not a consequence of altered function of the
type 1 ryanodine receptor , or of reduced
Ca ( 2 + ) stores , since the application of
4 - chloro - m - cresol , a direct
type 1 ryanodine receptor activator , elicited myoplasmic
Ca ( 2 + ) release in
YFP -
Rem - expressing myotubes that was not distinguishable from that in control myotubes . -
pubmed
However ,
YFP -
Rem reduced the magnitude of L - type
Ca ( 2 + ) current by approximately 75 % and produced a concomitant reduction in membrane - bound charge movements . -
pubmed
Thus , our results indicate that
Rem negatively regulates skeletal muscle EC coupling by reducing the number of functional L - type
Ca ( 2 + ) channels in the plasma membrane . -
pubmed
Blood pressure responses to the
nitric oxide synthase inhibitor
nitro - L - arginine - methyl - ester were evaluated . -
pubmed
CONCLUSION : The present data support the critical role of
AT ( 1A ) receptors in the development of 2K1C
hypertension , whereas
AT ( 1B ) receptors play only a minor role in blood pressure regulation in this model of
angiotensin II - dependent
hypertension . -
pubmed
Activation of
AT ( 2 ) receptors does not play an antagonistic role in the
AT ( 1 ) receptor - mediated
hypertensinogenic actions of
angiotensin II in this model . -
pubmed
A
cholecystokinin-1 receptor agonist (
CCK-8 ) mediates increased permeability of brain barriers to
leptin . -
pubmed
KEY RESULTS : We observed that
CCK-8 depressed the increase in plasma
leptin that followed the i.p . -
pubmed
The effect of
CCK-8 was totally prevented by the
CCK1 receptor antagonist ,
SR-27,897 ( 0.3 mg kg(-1 ) , s.c . ) -
pubmed
, but not by the
CCK2 receptor antagonist ,
L-365,260 ( 1 mg kg(-1 ) ) . -
pubmed
[
DMF - induced adult hepatocyte apoptosis and its effects on expression of
Bcl-2 ,
Bax and
Caspase-3 ] . -
pubmed
RESULTS : The increase in apoptotic rate of hepatocytes in concentration - manner was shown after
DMF treatment for 12 h. After treatment the expression of
Bcl-2 was decreased steadily and lower than the control group ( P & lt ; 0.01 ) , the expression of
Bax showed no significant difference among the groups of different dosage by one - factor analysis of variance ( P & gt ; 0.05 ) , as the increase of the dosage of
DMF . -
pubmed
The ratio of
Bcl-2 /
Bax dropped with the dosage of
DMF increasing , and the ratio in 200 mmol / L of
DMF was significantly lower than that of the control ( P & lt ; 0.01 ) . -
pubmed
CONCLUSION :
DMF can induce apoptosis of cultured adult normal hepatocytes in vitro , and the mechanism might be related to the decrease of
Bcl-2 /
Bax and the cleavage of
Caspase-3 . -
pubmed
H2S - donating
sildenafil (
ACS6 ) inhibits
superoxide formation and
gp91phox expression in arterial endothelial cells : role of
protein kinases A and G. -
pubmed
BACKGROUND AND PURPOSE :
Superoxide (
O ( 2 )(*- ) ) , derived from
nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase , is associated with
acute respiratory distress syndrome (
ARDS ) . -
pubmed
NADPH oxidase activity and expression are blocked by
nitric oxide (
NO ) and
sildenafil . -
pubmed
EXPERIMENTAL APPROACH : PAECs were incubated with 10 ng mL(-1 )
tumour necrosis factor - alpha (
TNFalpha ) ( + /-
NaHS or ACS6 ) , both of which released
H ( 2 ) S , for 2 h or 16 h.
O ( 2 )(*- ) was measured . -
pubmed
Expression of
gp91 ( phox ) was measured by western blotting and the role of
cyclic AMP (
cAMP ) and/or
cyclic GMP was assessed using
protein kinase inhibitors . -
pubmed
Both 100 nM
NaHS and 1 nM
ACS6 completely inhibited
gp91 ( phox ) expression induced by
TNFalpha . -
pubmed
The effects of
NaHS were blocked by the inhibition of
protein kinase A (
PKA ) , but not
PKG , and not by the inhibition of
guanylyl cyclase . -
pubmed
CONCLUSION AND IMPLICATIONS :
H ( 2 ) S inhibited
O ( 2 )(*- ) formation and upregulation of
NADPH oxidase in PAECs through the
adenylyl cyclase -
PKA pathway . -
pubmed
ACS6 may be effective in treating
ARDS through both elevation of
cAMP and inhibition of
phosphodiesterase type 5 activity . -
pubmed
METHODS :
Fos immunoreactivity revealing neuronal activation , and degenerating neurons labeled by
Fluoro - Jade B ( FJB ) histochemistry were analyzed on the first day after onset of
seizures lasting 3 h. Subpopulations of
gamma - aminobutyric acid (
GABA)ergic cells were characterized with double Fos -
parvalbumin immunohistochemistry . -
pubmed
To determine whether the observed bacterial adhesion to fish tissue cells was caused by non - specific interactions , adhesion to
bovine serum albumin (
BSA ) and
polystyrene was also tested . -
pubmed
Our results demonstrated that non - specific adhesion such as hydrophobic interactions are only partially involved in the binding process since adhesion to
BSA was low , and there was no correlation between adhesion to
polystyrene and adhesion to fish tissue cells . -
pubmed
The results showed that a region spanning 11
amino acids (
Ile(72)-
Thr(82 ) ) on
Glu1 is essential and sufficient for
BGAF binding , whereas the extreme N - terminal region
Ser(1)-Thr(29 ) , together with C - terminal region
Phe(466)-Ala(512 ) , affects the size of Glu1 -
BGAF complexes . -
pubmed
C-11 binds to
BGAF , indicating that the
Ile(72)-
Thr(82 ) region is indeed a major interaction site on Glu1 involved in
BGAF binding . -
pubmed
Dexamethasone treatment inhibits
VEGF production via suppression of
STAT3 in a
head and neck cancer cell line . -
pubmed
After
DEX treatment , the differences of
NF - kappaB (
p65 ) ,
glucocorticoid receptor and p -
AKT were not observed between the cell lines . -
pubmed
When
STAT3 was overexpressed after
DEX treatment , the level of
VEGF in the culture media was restored . -
pubmed
Taken together , we suggest that
p - STAT3 can be a mediating factor which regulates
VEGF secretion in the
DEX treatment . -
pubmed
Because the relationship between the three molecules DEX ,
STAT3 and
VEGF is scarcely known , our findings clarified one of the signaling pathways of
DEX , which is often used in clinical conditions . -
pubmed
MicroRNA-34a is induced via
p53 during
cisplatin nephrotoxicity and contributes to cell survival . -
pubmed
During
cisplatin treatment ,
p53 was activated . -
pubmed
Inhibition of
p53 with
pifithrin - alpha abrogated the induction of miR-34a during
cisplatin treatment of proximal tubular cells . -
pubmed
Collectively , the results suggest that miR-34a is induced via
p53 during
cisplatin nephrotoxicity and may play a cytoprotective role for cell survival . -
pubmed
Stable and fully functional complexes with different
CPR :
CYP3A4 stoichiometric ratios are formed within several minutes after addition of the full - length
CPR to the solution of
CYP3A4 preassembled into
POPC Nanodiscs at 37 degrees C. We find that the steady state rates of
NADPH oxidation and
testosterone hydroxylation strongly depend on
CPR :
CYP3A4 ratio and reach maximum at tenfold molar access of
CPR . -
pubmed
Defining the structural requirements for
ribose 5 - phosphate - binding and intersubunit cross - talk of the malarial
pyridoxal 5 - phosphate synthase . -
pubmed
Deletion analysis revealed that the C - terminus has four distinct functionalities : assembly of the
Pdx1 monomers , binding of the
pentose substrate (
ribose 5 - phosphate ) , formation of the reaction intermediate I(320 ) , and finally
PLP synthesis . -
pubmed
However , both the native and
camphor - soaked forms of
CYP101D2 had open conformations with an access channel . -
pubmed
Two other potential
camphor - binding sites were also identified from electron densities in the
camphor - soaked structure : one located in the access channel , flanked by the B / C and F / G loops and the I helix , and the other in a cavity on the surface of the
enzyme near the F helix side of the F / G loop . -
pubmed
HBsAg positive samples were further tested for
IgM anti -
HBc ,
HBeAg and
anti - HBe . -
pubmed
Specifically , 11.7 % were carriers of
HBsAg , whereas 28.8 % were negative for
HBsAg but positive for anti -
HBc . -
pubmed
Among the
HBsAg positive patients , 8.5 % were found positive for
HBeAg , while 5.1 % was positive for
IgM anti - HBc . -
pubmed
Endosulfan ( ED ) significantly increased the
hemoglobin concentration and white blood cell count after 96 h. Differential leukocytes count was also altered , due to an increase in the percentage of neutrophils in exposed fish . -
pubmed
Proinflammatory cytokines ,
chemokines ,
adhesion molecules and mediators of
inflammation including
inducible nitric oxide synthase ,
cyclooxygenase-2 and
nitric oxide orchestrate the inflammatory response . -
pubmed
Ginsenosides inhibit different inducers - activated signaling protein kinases and
transcription factor nuclear factor - kappaB leading to decreases in the production of
cytokines and mediators of
inflammation . -
pubmed
In this model , d / n -
ATF5 expression is controlled by
doxycycline and the promoter for
GFAP , a marker for stem / progenitor cells as well as
gliomas . -
pubmed
METHODS : Thirty - one peptides containing 15
amino acids each , which covered the full 222
amino acids of
Der p 1 protein sequence , were synthesized on the cellulous membrane by solid - phase peptide ( SPOTs ) synthesis , with 8 overlapping
amino acids between every two neighboring peptides . -
pubmed
RESULTS : Three strong
IgE - binding epitopes were identified in the
amino acid sequence of
Der p 1 molecule , namely Ep1 (
amino acids 85 - 99 ) , Ep2 (
amino acids 106 - 120 ) and Ep3 (
amino acids 190 - 204 ) . -
pubmed
The resulted composites showed uniform
palladium nanoparticles distribution , which were characterized by Fourier transform infrared spectrometry ( FTIR ) , transmission electron microscopy (
TEM ) , scanning electron microscopy ( SEM ) , Raman spectroscopy , atomic force microscopy (
AFM ) , X - ray diffraction spectroscopy ( XRD ) and electron diffraction pattern ( ED ) , etc . -
pubmed
MATERIALS AND METHODS : The nonfermenters were identified using a standard protocol that included tests for motility ,
oxidase production , oxidation - fermentation test for various
sugars , gelatin liquefaction , and growth on 10 %
lactose agar . -
pubmed
Influence of proteasome inhibitor
bortezomib on the expression of multidrug resistance genes and
Akt kinase activity . -
pubmed
The goal of this work was to study the mechanisms of
ABC family transport proteins ' regulation by a new - generation antitumor drug - the proteasome inhibitor
bortezomib (
Velcade ) . -
pubmed
We confirmed our previously discovered observation that
bortezomib affects the expression of genes involved in the formation of
MDR (
ABCB1 gene , also known as
MDR1 , and
ABCC1 -
MRP1 ) , reducing the amount of their mRNA . -
pubmed
When exposed to
bortezomib , selection of resistant to it cell variants was much faster for a
Pgp - overexpressing cell population ( compared to wild - type cells ) . -
pubmed
The data indicate that the transcriptional activity of
YB-1 might be one of the mechanisms that determine the effect of
bortezomib on the amount of
MRP1 gene mRNA . -
pubmed
Factors associated with OS were
bisphosphonates use ( HR = 0.5 , p = 0.008 ) , elevated pre - treatment
alkaline phosphatase ( HR = 2.9 , p = 0.003 ) and
sunitinib induced
HTN ( HR = 0.63 , p<0.0001 ) . -
pubmed
Since
ethylene receptors negatively regulate signaling , the suppression is canceled upon
ethylene binding , permitting responses including fruit ripening . -
pubmed
Treatment of preclimacteric fruits with
ethylene resulted in accumulation of
LeETR4 with reduced phosphorylation whereas treatments of ripening fruits with
ethylene antagonists ,
1 - methylcyclopropene and
2,5 - norbornadiene , induced accumulation of the phosphorylated isotypes . -
pubmed
Alteration in the phosphorylation state of receptors is likely to be an initial response upon
ethylene binding since treatments with
ethylene and
1 - methylcyclopropene rapidly influenced the
LeETR4 phosphorylation state rather than protein abundance . -
pubmed
MAIN OUTCOME MEASURES : Lateral thoracolumbar radiographs , bone mineral density (
BMD ) , bone turnover markers , and plasma
S1P levels were obtained from all subjects . -
pubmed
Induction of
heat shock proteins (
HSP ) represents an important adaptive response in muscle subjected to stress , and in several cell types including cardiac myocytes
prostaglandins are important in induction of the HSP response . -
pubmed
However , the
carbohydrate chain heterogeneity on the Fc region of
IgG (
Fc - IgG ) can play an important role in modulating the immune response . -
pubmed
Altogether , our results show that repeated
MDMA exposure causes strong neural and behavioral adaptations and that inhibitory feedback mediated by
alpha ( 2A )- adrenergic and 5 - HT ( 1A ) autoreceptors has an important role in the physiopathology of
addictive behaviors . -
pubmed
The approach is demonstrated in the modification of a
quartz surface and in the incorporation of an
azobenzene unit into a functional peptide , the third
zinc finger in the
mammalian factor Sp1 . -
pubmed
It is known that the expression of
DIO1 , a gene contributing to
triiodothyronine ( T3 ) signalling , is regulated by miR-224 . -
pubmed
Thus , we analysed mutual regulation between
triiodothyronine pathway and miR-224 / miR-452 /
GABRE cluster . -
pubmed
Firstly , we found that miR-452 directly regulates the expression of
thyroid hormone receptor
TRbeta1 in
renal cancer cells . -
pubmed
Previous reports used
granulocyte - colony - stimulating factor (
G - CSF ) for mobilization of stem cells ; there are no reported cases undergoing
plerixafor mobilization . -
pubmed
Since
cinnarizine is a well - tolerated
calcium channel blocker which has acceptable effect on both
vertigo and
migraine headache , we carried out this study to evaluate the efficacy and safety of this medication in
vestibular migraine and also
migraine with brainstem aura associated with
vertigo . -
pubmed
Development of a
Ru complex - incorporated
MOF photocatalyst for
hydrogen production under visible - light irradiation . -
pubmed
A
Ru complex - incorporated
Ti - based
MOF (
Ti - MOF - Ru ( tpy ) 2 ) has been synthesised by using a
bis ( 4 '-( 4 - carboxyphenyl )- terpyridine ) Ru ( ii ) complex
( Ru ( tpy ) 2 ) as an organic linker . -
pubmed
The solubility of
BCS class
II compound
carvedilol was found to increase significantly in simulated and aspirated media of the fed state . -
pubmed
,
alpha - , beta - DBE - DBCH ,
BATE ,
PBEB ,
PBT ,
TBP - DBPE ) were measured close to or below the limit of quantitation ( 0.64 ng / g ) in both the NCCLS and FOX samples , and the seventh ,
HBB , was measured at median concentrations of 1.85 ng / g and 9.40 ng / g in the NCCLS and FOX samples , respectively . -
pubmed
Histone
H2B ubiquitination at
lysine 120 (
H2Bub1 ) is regulated by
RNF20 , an
E3 ubiquitin ligase that is altered in many
tumor types . -
pubmed
Through an evolutionarily conserved trans - histone pathway ,
H2Bub1 is an essential prerequisite for subsequent downstream dimethylation events at
lysines 4 ( H3K4me2 ) and 79 ( H3K79me2 ) of histone
H3 . -
pubmed
This results in the processing of
high - molecular - weight kininogen and the release of
bradykinin , a potent vascular mediator . -
pubmed
The changes in
Tph expression was in agreement with the levels of
serotonin ( 5 -
HT ) and
5 - hydroxytryptophan in the preoptic area - hypothalamus . -
pubmed
Considering the stimulatory influence of
5 - HT on
GnRH and
GTH release , it is possible to propose a network association between these correlates during early development , which may bring about brain sex dimorphism in males . -
pubmed
A recent study from our laboratory during female brain sex development demonstrated high expression of
tyrosine hydroxylase in correlation with
catecholamine levels , brain
aromatase and its related transcription factors such as
fushi tarazu factor 1 ,
Ftz - f1 and
fork head box protein L2 ,
foxl2 . -
pubmed
Phase
II Trial of
S-1 and
Oxaliplatin Plus Cetuximab for
Colorectal Cancer Patients with Initially Unresectable or Not Optimally Resectable Liver Metastases ( KSCC1002 ) . -
pubmed
Overall these studies reveal core mechanisms fundamental to grafted NPC - based therapeutic effects , and delineate the particular contributions of two graft - expressed molecules ,
SHH and
GDNF , in mediating midbrain
dopamine neuron protection , and host plasticity after NPC transplantation . -
pubmed
The biotransformation of the nanoparticle
iron oxide (
maghemite ) core into
ferritin , the non - toxic form of
iron storage , was demonstrated for the first time ex vivo in
atherosclerotic plaques as well as in spleen , the
iron storage organ . -
pubmed
This study showed that these nanoparticles were stored as non - toxic
iron compounds :
maghemite oxide or
ferritin , which is promising for MRI detection of
atherosclerotic plaques in clinics using these USPIOs . -
pubmed
Thirteen isolates were found to be
ciprofloxacin - resistant , and all harbored a Ser84Leu mutation within the QRDR of the
GyrA protein , with 3 isolates showing 2 extra substitutions , Ser98Ile and Arg100Lys ( one
strain ) and Glu88Asp and Asp96Thr ( 2
strains ) . -
pubmed
The replacement of
Asn203 with a basic
amino acid was suggested to stabilize alpha - helix 2 , which is important for the
Mpr1 structure , probably by neutralizing its dipole . -
pubmed
In addition , the combination of two
amino acid substitutions at positions 65 and 203 in
Mpr1 , Phe65Leu , which was previously isolated by the screening from PCR random mutagenesis library of
MPR1 , and Asn203Lys or Asn203Arg , led to further stabilization of
Mpr1 . -
pubmed
Together , these results suggest that striatal
MRS measures of
lactate or
glutathione might be useful biomarkers for early detection of risk for developing
compulsive behavior disorders . -
pubmed
These findings suggest that adipocyte
COX-2 PGE2 -
EP3 - mediated signaling is crucially involved in the development of
obesity - associated AT
inflammation and
insulin resistance.-Chan , P.-C. , Hsiao , F.-C. , Chang , H.-M. , Wabitsch , M. , Hsieh , P. S. Importance of adipocyte
cyclooxygenase-2 and
prostaglandin E2 - prostaglandin E receptor 3 signaling in the development of
obesity - induced
adipose tissue inflammation and
insulin resistance . -
pubmed
Association between estimated blood
glucose levels and
glycated hemoglobin levels . -
pubmed
Tissue specific expression of genes encoding
cholesterol transporters
ABCA1 and
ABCG1 as well as genes encoding the most important transcriptional regulators of adipogenesis -
LXRa ,
LXRb ,
PPARg and
RORa has been investigated in intraabdominal adipose tissue ( IAT ) samples . -
pubmed
These data suggest that the nuclear factor
RORa can play a significant role in the regulation of
cholesterol metabolism and control IAT expression of
ABCA1 and
ABCG1 , while the level of IAT
LXRb gene expression may be an important factor associated with the development of
obesity . -
pubmed
INTRODUCTION :
Anaplastic lymphoma kinase ( ALK ) rearranged lung adenocarcinomas are responsive to the multitargeted
ALK inhibitor
crizotinib . -
pubmed
One of the common mechanisms of resistance to
crizotinib is the acquisition of
ALK kinase domain mutations . -
pubmed
However , the presence of
ALK mutations in
crizotinib - naive
tumors has not been widely reported and it is unclear if de novo
ALK mutations affect the response to
crizotinib . -
pubmed
METHODS : We analyzed preclinical models of
ALK rearranged lung cancers that were sensitive / resistant to
ALK inhibitors , probed our institutional and other
lung cancer databases for
tumors with
ALK kinase domain mutations , and evaluated
tumor response to
crizotinib . -
pubmed
RESULTS :
ALK rearranged cell lines with
ALK kinase domain mutations were heterogeneously less inhibited by increasing concentrations of
crizotinib than cells driven solely by
EML4 -
ALK fusions . -
pubmed
We identified one TKI - naive
ALK rearranged
tumor with an
ALK kinase domain mutation :
ALK - S1206F ( mutations at
ALK - S1206 shifted
crizotinib inhibitory curves only minimally in preclinical models ) . -
pubmed
The never smoker whose
tumor harbored de novo
EML4 -
ALK - E5;
A20+
ALK - S1206F only achieved a 4 - month radiographic response to
crizotinib 250 mg twice daily . -
pubmed
In addition to producing antibody , Bregs also regulate diseases via
cytokine production and
antigen presentation . -
pubmed
Chicory
inulin ameliorates
type 2 diabetes mellitus and suppresses
JNK and
MAPK pathways in vivo and in vitro . -
pubmed
We used a combination of Raman microscopy ,
AFM and
TEM to quantify the influence of dimerization on the surface enhanced Raman spectroscopy ( SERS ) signal for gold and
silver nanoparticles ( NPs ) modified with Raman reporters and situated on gold ,
silver , and
aluminum films and a
silicon wafer . -
pubmed
We determined the involvement of afferent sensory neurons ,
calcitonin - gene - related peptide (
CGRP ) , lipid peroxidation , and
nitric oxide (
NO ) biosynthesis in gastroprotection of
H2S - releasing
NaHS and
CO - releasing
tricarbonyldichlororuthenium ( II ) dimer (
CORM-2 ) against
aspirin - induced
injury . -
pubmed
The areas of
aspirin - induced lesions and gastric blood flow (
GBF ) were assessed by planimetry and laser flowmetry respectively . -
pubmed
RESULTS :
Aspirin caused
gastric lesions , decreased
GBF , and raised
MDA content , but pretreatment with
NaHS and
CORM-2 reduced these effects . -
pubmed
NaHS and
CORM-2 decreased
MDA and
4 - HNE content , restoring
GPx-1 protein expression . -
pubmed
NaHS and
CORM-2 prevented
aspirin - induced gastric mucosal lipid peroxidation via restoration of microcirculation and antioxidative
GPx-1 protein expression . -
pubmed
Conversely , the addition of
APLN-13 and
APLN-17 to in vitro maturation medium containing
IGF1 ( 10 - 8 M ) but not
FSH ( 10 - 8 M ) arrested most oocytes at the germinal vesicle stage , which was associated with a decrease in
progesterone secretion , an inhibition in
MAPK ERK1 / 2 phosphorylation and an increase in
PRKA phosphorylation in oocytes . -
pubmed
Particularly ,
lupane - type
triterpenes have shown great ability to modulate multiple
cancer - related signaling pathways and processes , including
NF - kappaB , Wnt /
beta - catenin ,
PI3K /
Akt , apoptosis , and many other routes related to proliferation or cell
death , which are uncontrolled in
malignant tumors . -
pubmed
NMR data were used to construct a three - dimensional structural model of the
CCL2 /
CCR218 - 31 complex , revealing that
CCR218 - 31 occupies a binding site juxtaposed to the dimer interface , partially replacing monomer - monomer contacts , explaining why CCR218 - 31 binding weakens the dimer interface and induces dissociation . -
pubmed
Expert opinion :
Baricitinib is an oral targeted synthetic ( ts ) disease - modifying antirheumatic drug ( DMARD ) that mainly inhibits
JAK1 and
JAK2 . -
pubmed
These occurrences are indicative of an expansion of the
citrulline - specific B cell response , though
ACPA remain of low avidity even in established disease . -
pubmed
Before
PMX - DHP , the survivor group exhibited a significantly higher PaO2 / FiO2 ( P / F ) ratio and serum
surfactant protein - D (
SP - D ) levels and significantly lower
lactate dehydrogenase (
LDH ) and
ferritin levels than the non - survivor group . -
pubmed
Tumor Cell - selective Synergism of
TRAIL- and
ATRA - induced
Cytotoxicity in
Breast Cancer Cells . -
pubmed
The present work focused on
tumor cell - inhibiting effects of a combination of
tumor necrosis factor - related apoptosis - inducing ligand (
TRAIL ) and
all trans retinoic acid (
ATRA ) in
breast cancer cells . -
pubmed
Cell viability was assessed via
3 -( 4,5 )- dimethylthiahiazo (- z - yl )- 3,5 - di - phenytetrazoliumromide (
MTT ) assay , the potential of cell colony formation via clonogenic assay , cell
death induction via cell - cycle analysis by fluorescence - activated cell sorting ( FACS ) ,
terminal deoxynucleotidyltransferase - mediated UTP nick end labeling ( TUNEL ) assay and Cell
death detection ELISAPLUS , expression of apoptosis and
TRAIL pathway proteins via western blot and cell surface expression of
TRAIL receptor 1 (
DR4 ) via FACS analysis . -
pubmed
RESULTS :
TRAIL and
ATRA evoked synergistic inhibition of
breast cancer cell viability based on cytostatic and cytotoxic mechanisms . -
pubmed
CONCLUSION : The cytostatic and
cytotoxic effects of the combination of
TRAIL and
ATRA are
tumor cell - selective . -
pubmed
Each of
NMT and
terazosin has its own advantages in attenuating
urinary tract irritation and obstruction , but whether their combination may produce a better effect on
LUTS and the specific mechanisms of NMT improving acute symptoms of
BPH are yet to be further studied . -
pubmed
Most frequent pore size in GCT 30 scaffold was the highest of 120 mum whereas that for
GCH 30 was the lowest of 96 mum as suggested by
Hg porosimetry analysis . -
pubmed
Among those in the
MAD arm who generated at least trace amounts of urinary
ketones , there was a large ( effect size = 0.53 ) and statistically significant ( p = 0.03 ) increase in Memory Composite Score between the baseline and week-6 assessment . -
pubmed
The determination of the
albumin -
creatinine ratio in
spot urine is easy to perform and the method of choice to diagnose
nephrotic - range
proteinuria . -
pubmed
Also examined were whether effects on adrenal steroidogenesis that were not obvious in the blood
steroid levels after sole administration of
KET could be revealed by
post - administration of
ACTH , and the correlation between the blood and adrenal
steroid levels . -
pubmed
KET - induced changes in blood levels of other
steroids were revealed by
ACTH , and the blood and adrenal levels were generally correlated especially after
ACTH post - administration . -
pubmed
The usefulness of the multiple
steroid measurement as a method for mechanism investigation of drug effects on the adrenal gland can be further enhanced by
ACTH . -
pubmed
In February 2016 , because follow - up
CT revealed a right
adrenal tumor with time - dependent growth ,
sunitinib ( 25 mg / body ) was introduced . -
pubmed
Vitamin treatments in the commercial diluent were as follows : 2.4 mug of
vitamin D3 (
D3 ) or
25 - hydroxylcholecalciferol (
25OHD3 ) . -
pubmed
The ET of eggs significantly fluctuated during the postinjection time period ; however , the type of
vitamin D3 source injected did not affect
ET . -
pubmed
Nevertheless , the injection of
25OHD3 resulted in a lower late embryo mortality than the diluent and
D3 injection treatments . -
pubmed
In conclusion , the in
ovo injection of
25OHD3 has the potential to improve early posthatch broiler performance without affecting
ET . -
pubmed
The aim of this study is to investigate pharmacokinetic ( PK ) characteristics of inhaled
FTS , SM , and
TTP after co - administration . -
pubmed
A single dose of
FTS / SM ( 250/50 microg ) and
TTP ( 18 microg ) were concomitantly inhaled in period 1 , and the dose of each drug was escalated to two- and three - fold in
periods 2 and 3 , respectively , with a 2 - week washout between periods . -
pubmed
In conclusion , PK characteristics of
FTS , SM , and
TTP by pulmonary absorption were evaluated after concurrent inhalation . -
pubmed
In this study , we developed a hydrogel - blended scaffold consisted of gelatin
methacryloyl (
GelMA ) and alginate enriched with
hydroxyapatite nanoparticles (
HAP ) to model an in vitro prevascularized bone construct . -
pubmed
Objectives :
C - reactive protein (
CRP ) is an acute phase protein used in multiple canine inflammatory conditions including
steroid responsive
meningitis -
arteritis ,
immune - mediated polyarthritis and
bronchopneumonia . -
pubmed
Here a novel
MN patch is designed in which the MNs are coated with
insulin /
poly - l - glutamic acid (
PGA ) layer - by - layer ( LBL ) films at pH 3.0 . -
pubmed
The new
MN patch is expected to become a painless , convenient method for the control of postprandial
glucose . -
pubmed
Since
HIF-1alpha and
VEGFA are critical factors promoting alveolar development and pulmonary angiogenesis , these results suggested that
PFOS may also affect lung development by inhibiting
HIF-1alpha and
VEGFA expression . -
pubmed
The primary endpoint was changes in cardio - metabolic risk based on the Suita score at 1 year , and the secondary endpoints were changes in medical examination data related to cardio - metabolic risk ( total
cholesterol [ TC ] ,
triglyceride ,
high - density lipoprotein cholesterol ,
low - density lipoprotein cholesterol ,
body mass index [
BMI ] , and
hemoglobin A1c ) at 1 year . -
pubmed
The bread was fortified with protein ( whey protein , blood plasma proteins from farm animals ) , dietary fiber ,
calcium ( eggshell ) ,
iron ( purified
hemoglobin ) and iodized whey protein . -
pubmed
Blood levels of total
calcium ( by colorimetric method ) ,
gonadotropins ,
testosterone , and
estradiol ( by enzyme - linked immunosorbent assay ) were analyzed . -
pubmed
In animals of the O120 + group , in comparison with the O120 sample , there was a decrease in blood
testosterone and a marked compensatory release of
follicle - stimulating hormone , while no changes were detected in the concentration of
estradiol and the state of the uterus atrophied against the background of ovariectomy . -
pubmed
These changes are not specific for
acrylamide intoxication , having been observed in other experimentally induced
neuropathies (
n - hexane ,
Mn - BK ,
CS2 , ... ) , as well as in a variety of diseases both genetically determined and due to exposure to toxic substances ( glue - sniffing , leather cement
poisoning , antiblastic therapy , ... ) . -
pubmed
A comparative study of antiestrogen action : temporal patterns of antagonism of
estrogen stimulated uterine growth and effects on
estrogen receptor levels . -
pubmed
Changes in the chemical structures of these anti -
estrogens produce compounds with markedly different affinities for the
uterine estrogen receptor as measured in an in vitro cell - free cytosol system ; binding affinities relative to
estradiol ( 100 % ) are :
CI-628 , 4 % ;
CI-680 , 34 % ; 94X1127 ( 94X),222 % ; U-11,100A ( UA ) , 6 % ; and
U-23,469 ( U-23 ) , 0.1 % . -
pubmed
The compounds
CI-628 ,
CI-680 and
UA ( 50 microng sc in saline ) , which have a
methylated hydroxyl group ( at the site analogous to the
steroid position 3 ) , show a prolonged maintenance of elevated levels of
nuclear receptor ( beyond 48 h ) and elevated uterine weight ( until 72 h ) ; this correlates with a prolonged period of depressed cytoplasmic receptor levels ( beyond 48 h ) and prolonged uterine insensitivity to
estrogen ( beyond 36 h as monitored by 3 h wet weight response ) . -
pubmed
Comparative studies with these five compounds indicate that they are effective as
estrogen antagonists only so long as they maintain cytoplasmic receptor levels low , and that the magnitude of the responsiveness to
estradiol after antiestrogen correlates with the extent of reappearance of
cytoplasmic receptor . -
pubmed
Histamine release is induced by
monoclonal antibodies directed against the Fab but not the Fc region of the
IgG4 molecule . -
pubmed
That
HR is
calcium dependent and is accompanied by the synthesis and release of
leukotriene C4 . -
pubmed
In addition , we found that nine donors were unresponsive to an anti -
IgE stimulus , while they released
histamine efficiently after challenge with anti -
IgG4(
Fab ) , suggesting the existence of different receptors for both
immunoglobulins . -
pubmed
Frequent expression of
CD19 was found in the blastic population of t(8;21 )
AML ( 18 of 23 cases ) without other B - cell
antigens and Ig gene rearrangements . -
pubmed
Our findings indicate that leukemic blasts of t(8;21 )
AML commonly express
CD19 while preserving the stem cell - associated
antigens , and differentiate into the granulocytic pathway with discordant maturation such as low
CD33 expression . -
pubmed
Neurokinin A (
NKA ) caused dose - dependent increases in ciliary beat frequency (
CBF ) , as measured by a photoelectric method , with the maximal increase from the baseline 15.7 + /- 1.7 % ( mean + /- SEM , p less than 0.01 ) , whereas
substance P ( SP ) had no effect . -
pubmed
The NKA - induced increase in
CBF was not inhibited by
phentolamine ,
propranolol , or
atropine , but it was abolished by the
tachykinin antagonist
[ D - Pro2 , D - Trp7,9 ] SP . -
pubmed
Pretreatment of tissue with
thiorphan ( 10(-5 ) M ) , a
NEP inhibitor , had little effect on
CBF responses to NKA ; however , it significantly potentiated the responses to SP ( 14.9 + /- 3.0 % , p less than 0.01 ) . -
pubmed
Other
peptidase inhibitors , including
captopril ,
bestatin , and
leupeptin , did not alter the
tachykinin - induced
CBF response , suggesting that
angiotensin converting enzyme ,
aminopeptidases , and
serine proteinases do not modulate ciliary activity in response to
tachykinins . -
pubmed
These results suggest that
NKA increases
CBF by acting directly on
tachykinin receptors and that
NEP may play a role in modulating the
tachykinin - induced stimulatory effects on
CBF . -
pubmed
Calcitriol as well as
angiotensin - converting enzyme could represent unregulated secretion products from granulomatous tissue and their plasma concentrations may roughly reflect activity of the disease . -
pubmed
The mechanism of the sensitizer - membrane interactions has been studied by following the distribution properties of selected
porphyrins , including
haematoporphyrin (
HP ) and
protoporphyrin (
PP ) , into unilamellar liposomes of
dipalmitoyl phosphatidylcholine (
DPPC ) . -
pubmed
At
porphyrin concentrations below 0.5 microM ,
HP and
PP exclusively localize in the inner phospholipid monolayer ; at higher concentrations , the outer monolayer also becomes populated . -
pubmed
The
porphyrin binding sites in liposomes , however , are different for
HP and
PP :
HP preferentially distributes into water - accessible lipid regions , while
PP localizes in the most hydrophobic loci of the lipid matrix . -
pubmed
In
DPPC -
cholesterol vesicles , all
HP molecules dissolve in
DPPC - rich regions while all
PP molecules partition in
cholesterol - rich environments . -
pubmed
While the raised
GGT values reduced gradually , the MCV values remained consistently high even after strict withholding of
alcohol . -
pubmed
We have distinguished the
InsP4 binding site from the
InsP3 receptor binding site by differences in brain regional and tissue distribution , affinity for
InsP4 and
InsP3 , and sensitivity to
calcium . -
pubmed
Secretion of
lysosomal beta - glucuronidase ,
membrane 5 '- nucleotidase ,
cellular plasminogen activator , and
superoxide anion generation were measured as markers of activation . -
pubmed
On the basis of the 5'-nucleotidase findings , it is concluded that both the
NC and HC
granuloma macrophages are highly activated , and further that
hypercholesterolemia does not enhance macrophage generation of
superoxide anion , either spontaneously or as the result of
phorbol myristate acetate stimulation . -
pubmed
Hepatocellular and Kupffer cell lysosomes were engorged with
ferritin and contained clefts and trilaminar structures believed to represent very long chain
fatty acids . -
pubmed
Phorbol esters broaden the action potential in
CA1 hippocampal pyramidal cells . -
pubmed
O , O ' , S - tris - acetyl-3 - thio - hydroquinone , which was characterized by
GC /
MS . -
pubmed
1.5 % of the administered dose of
APAP after 24 h , a figure which is equivalent to
6.3 % of the corresponding
APAP -
thiol conjugates in the urine . -
pubmed
The tetrapeptide
FMRF -
HN2 hyperpolarizes the pacemaker neurons . -
pubmed
Identification of a
hydroxide ligand at the
iron center of
ribonucleotide reductase by resonance Raman spectroscopy . -
pubmed
Its exact location in the
16O species is obscured by the presence of a
protein tryptophan vibration at 758 cm-1 . -
pubmed
A model for the binuclear
iron center of
ribonucleotide reductase is presented in which the
hydroxide ligand sites provide an explanation for the half - of - sites reactivity of the
enzyme . -
pubmed
Rifampicin resistance of
RNA polymerase in the binary complex with DNA . -
pubmed
The same treatment with
TA-064 elevated the concentrations of blood
FFA ,
glycerol and plasma IRI and
IRG . -
pubmed
These changes induced by
TA-064 were inhibited by pretreatment with
propranolol ( a non - selective beta - adrenergic antagonist ) and
practolol ( a selective
beta 1 - adrenergic antagonist ) . -
pubmed
They also brought about sustained rises in blood
glycerol and plasma IRI , but only transiently increased the plasma
IRG level . -
pubmed
The cardiotonic agent
prenalterol , claimed to be a selective
beta 1 - agonist , elevated blood
glucose ,
lactate , and
glycerol only slightly , and plasma IRI significantly , but it had no effect on plasma IRG . -
pubmed
Stimulation of meiosis resumption in vitro in follicle - enclosed oocytes by both
LH and
[ D - Ala6 ] GnRH , was blocked by elevated levels of
cAMP as demonstrated when either
dibutyryl cAMP or the
phosphodiesterase inhibitor
methylisobutylxanthine was present in the culture medium . -
pubmed
On the other hand , the potent
GnRH - antagonist
[ D - pGlu1 , pClPhe2 ,
D - Trp3,6 ] GnRH which blocked the stimulatory effect of the agonist on oocyte maturation and ovulation had no effect on
LH action . -
pubmed
It is concluded that while a
GnRH - like peptide does not seem to mediate
LH action on the ovarian follicles , both
LH and
GnRH agonist share some common mechanistic pathways at a
post - receptor locus . -
pubmed
Since
1 - O - alkyl-2 - lyso - GPC is the metabolic precursor of
platelet activating factor , these results further interrelate pathways forming
arachidonate metabolites and
platelet activating factor ; they also supply a rationale for the observation that both classes of stimuli form concomitantly during cell activation . -
pubmed
Experiments designed to test whether
Ca2 + mediates metabolic crenation showed that : ( 1 ) an influx of extracellular
Ca2 + is not required for metabolic crenation ; ( 2 ) metabolic crenation is accompanied by a 70 % increase in
86Rb + permeability , a change much smaller than the increase expected if crenating concentrations of
Ca2 + were released from bound intracellular pools ; ( 3 )
A23187 plus
EGTA , a treatment that depletes intracellular
Ca2 + and stops
Ca2 + crenation , does not affect metabolic crenation ; ( 4 )
calmodulin inhibitors do not slow metabolic crenation . -
pubmed
Albumin washes reverse
Ca2 + crenation and metabolic crenation involve the accumulation of some amphiphilic species ( e.g. -
pubmed
These findings indicate that
sulfido derivatives of
CP can act directly ( without metabolic activation ) on cells , probably through spontaneous stepwise conversion to
phosphoramide mustard , the presumed proximal alkylating agent . -
pubmed
Sulfidocyclophosphamide CP derivatives appear to be suitable for in vitro studies of the mechanism of action of
CP . -
pubmed
Sulfidocyclophosphamide CP derivatives may also have therapeutic potential as
CP - like drugs that do not require metabolic activation . -
pubmed
The following agents were evaluated in the absence of
S9 :
1 - nitropyrene , diesel exhaust extract , and the chlorinated drinking water mutagen
3 - chloro-4 - dichloromethyl-5 - hydroxy-2 -[ 5H ]- furanone ( MX ) . -
pubmed
In the presence of
S9 , the following agents were evaluated :
4 - aminobiphenyl ,
benzo [ a ] pyrene , and an organic extract from the particulate emissions resulting from the combustion of
polyethylene in a rotary kiln incinerator . -
pubmed
MEASUREMENTS :
Serum GH and
IGF - I were measured by radioimmunoassay whilst
uGH was estimated by an immunoradiometric assay using commercially available
reagents . -
pubmed
Angiotensin - converting enzyme (
ACE ;
EC 3.4.15.1 ) may participate in
respiratory inflammatory diseases by regulating levels of inflammatory peptides such as
bradykinin . -
pubmed
ACE activity was significantly more abundant in the membrane - rich fraction than in the soluble cytosolic fraction of nasal mucosal extracts ( 74.18 + /- 24.50 versus 3.99 + /- 1.83 pmol / min / mg protein , respectively , P & lt ; 0.01 by an
enkephalin degradation assay ; 89.16 + /- 16.17 versus 2.30 + /- 0.89 mU / mg protein , P & lt ; 0.01 by colorimetric assessment of
Bz - Gly - Gly - Gly degradation ) . -
pubmed
Topical application of
histamine stimulated secretion of
ACE activity into nasal lavage fluid ( 2.90 + /- 0.88 versus 1.53 + /- 0.45 U / liter after saline provocation , P & lt ; 0.05 by
Bz - Gly - Gly - Gly assay ) . -
pubmed
In both
histamine and allergen challenges ,
ACE release correlated closely with that of the vascular proteins
IgG and
albumin . -
pubmed
Methacholine , a stimulant of glandular secretions , failed to augment
ACE levels above baseline . -
pubmed
ACE - immunoreactive material was localized by the immunogold technique with
silver enhancement to the glycocalyx , between epithelial cells , and to interstitial , extracellular sites in the superficial lamina propria , with the highest intensity of staining immediately beneath the basement membrane . -
pubmed
ACE was identified by immunoblotting as a 150 kD band on
SDS - PAGE. -
pubmed
Mechanism of the
acyl - carbon cleavage and related reactions catalyzed by multifunctional
P-450s : studies on
cytochrome P-450 ( 17 ) alpha . -
pubmed
It is now well - known that conventional
cytochrome P-450s catalyze hydroxylation reactions using an
iron mono - oxygen species , the structure of which , as inferred from chemical model studies , may be drrepresented by the following canonical forms : FeV==O<-->(.+)FeIV==O<-->
FeIV -- O ( . - pubmed
Certain multifunctional
P-450s , notably those involved in
steroid biosynthesis , catalyze , in addition to hydroxylation reactions , an
acyl - carbon cleavage process in which the participation of an
iron peroxide intermediate ,
FeIII -- OOH , has been suggested . -
pubmed
1,3 - Dihydro-5 - phenyl-2H - pyrido [ 3,2 - e ]- 1,4 - diazepin-2 - ones ( 11 - 13 ) , in which the
chlorophenyl ring of
7 - chloro-1,4 - benzodiazepin-2 - ones is replaced by a
pyridyl ring , were synthesized and evaluated as anticonvulsants using subcutaneous
pentylenetetrazole (
scPTZ ) and maximal electroshock (
MES ) induced
seizure screening tests . -
pubmed
A
methyl substituent at the N-1 and/or C-3 position of the
diazepin-2 - one ring system was not a determinant of scPTZ activity , but the
1,3 - dimethyl derivative ( 13 ) was more potent than the parent
1,3 - unsubstituted compound ( 11 ) in the
MES screen . -
pubmed
TAM resistance was not related to the development of
estrogen receptors , decreased
TAM uptake , or the increased expression of the
mdr-1 gene . -
pubmed
The purpose of this study was to analyze the effects of
nordihydroguaiaretic acid , an inhibitor of the
lipoxygenase pathway of
arachidonic acid , on antibody - dependent cellular
cytotoxicity . -
pubmed
The inhibition produced by
nordihydroguaiaretic acid could not be reversed by
leukotriene B4 , a
5 - lipoxygenase product . -
pubmed
We demonstrate that
small heat shock proteins (
sHsp ) inhibit in vitro amyloid formation by the Alzheimer 's
A beta ( 1 - 42 ) polypeptide as detected by a
thioflavine T fluorescence assay and electron microscopy . -
pubmed
Proteins of the
ATF /
CREB class of
transcription factors stimulate gene expression of several cell growth - related genes through
protein kinase A - related
cAMP response elements . -
pubmed
However , numerous examples of self - catalyzed protein rearrangements have recently been discovered , including protein splicing and autoproteolysis of
hedgehog proteins ,
glycosylasparaginases and
pyruvoyl enzyme precursors . -
pubmed
An experiment was performed to evaluate the effects of
somatotropin on plasma free
amino acid ,
urea and
insulin concentrations and rumen fermentation pattern and to assess their relationships . -
pubmed
Exogenous
rbST increased plasma
insulin concentration and the
insulin response to feeding , and decreased plasma
urea and free essential and branched chain
amino acid concentrations . -
pubmed
AIM : To examine whether
alpha - hederin (
He d ) modulates hepatic detoxifying systems as a means of hepatoprotection . -
pubmed
RESULTS : He d increased liver
glutathione (
GSH ) content ( 20 % ) , but had no effect on
GSH peroxidase ,
GSH reductase , and
GSH S - transferase . -
pubmed
The activities of
superoxide dismutase and
quinone reductase were unaffected by
He d treatment . -
pubmed
At the high dose of
He d ,
catalase activity was decreased by 20 % . -
pubmed
Hepatic content of
metallothionein was dramatically increased ( 50 - fold ) , along with elevations of hepatic
Zn and
Cu concentrations ( 25%-80 % ) . -
pubmed
Neuropeptide receptors couple via G - proteins to two principal signaling pathways that elevate
cAMP through
adenylate cyclase ( AC ) or mobilize intracellular
Ca ( 2 + ) through
phospholipase C ( PLC)-stimulated
inositol phosphate (
IP ) turnover and production of
inositol 1,4,5 - trisphosphate (
IP ( 3 ) ) . -
pubmed
We showed previously that high - affinity receptors for
pituitary adenylate cyclase - activating polypeptide (
PACAP ) are present on chick ciliary
ganglion neurons and that receptor occupation increases
cAMP production , resulting in enhanced
acetylcholine sensitivity . -
pubmed
After we suppressed AC activity and
cAMP production with
2 '- 5 ' dideoxyadenosine , however ,
PACAP no longer increased
acetylcholine sensitivity but instead reduced it , suggesting that an AC - independent signal pathway activated by
PACAP inhibits some
nicotinic acetylcholine receptors (
AChRs ) . -
pubmed
Without previous AC block , both the rapidly desensitizing , alpha - bungarotoxin ( alphaBgt)-sensitive
alpha7 - AChRs and the slowly desensitizing , alphaBgt - insensitive
alpha3 * - AChRs on the neurons were potentiated by
PACAP . -
pubmed
After AC blockade , however ,
PACAP inhibited
alpha7 - AChRs but left
alpha3 * - AChRs unaffected . -
pubmed
The selective inhibition of
alpha7 - AChRs appeared to use a
PLC signaling pathway because it was not seen after lowering
PLC activity or buffering intracellular
Ca ( 2 + ) and was mimicked by dialyzing neurons with an
IP ( 3 ) receptor agonist . -
pubmed
PACAP also induced
IP turnover and increased [
Ca ( 2 +)]( i ) assessed directly with
Fluo-3AM imaging . -
pubmed
In male brains ,
PGE2 - 9 - ketoreductase inhibitor decreased
PGF2alpha release and increased that of
PGE2 ;
aromatase inhibitor decreased
17beta - oestradiol and increased
testosterone release . -
pubmed
In female brains ,
NO donor and
PGE2 - 9 - ketoreductase inhibitor increased
testosterone and
PGE2 release and decreased that of
17beta - oestradiol and
PGF2alpha ;
NO synthase inhibitor decreased
testosterone release and
PGE2 and increased
17beta - oestradiol and
PGF2alpha release ;
PGF2alpha decreased
testosterone release and increased
17beta - oestradiol release ;
aromatase inhibitor decreased
17beta - oestradiol release and increased
testosterone release . -
pubmed
In female brains ,
NO donor and
PGE2 - 9 - ketoreductase inhibitor decreased
PGE2 - 9 - ketoreductase and
aromatase activities ;
PGF2alpha increased
aromatase activity ;
NO synthase inhibitor increased
PGE2 - 9 - ketoreductase and
aromatase activity . -
pubmed
The data suggest that , in amplexing female brains , external and/or internal stimuli inhibit
NO synthase , decreasing
NO and activating
PGE2 - 9 - ketoreductase ; in turn ,
PGF2alpha increases
aromatase activity and
17beta - oestradiol release ; while , in amplexing male brains , stimuli inhibit
aromatase activity , thereby increasing
testosterone production . -
pubmed
In particular , differences observed in subsites S(3 ) , S(4 ) and the loops involved in inhibitor interactions explain the preference of
caspase-8 for substrates with the sequence (
Leu / Val )- Glu - X - Asp . -
pubmed
We developed buffer conditions that suppressed dynamic instability behavior by approximately 10 - fold to minimize the contribution of dynamic instability to total
tubulin -
GTP exchange . -
pubmed
This system was used to demonstrate that
soxS expression is sufficient for the induction of resistance to the
superoxide - generating drug
paraquat and for the transcriptional activation of the
sodA and
micF genes . -
pubmed
This selection yielded six independent chromosomal
lacZ transcriptional fusions that were activated by either artificial expression of
SoxS or exposure of wild - type cells to micromolar concentrations of
paraquat . -
pubmed
These systems consisted of a homologous series of
n - alkanes as both analytes and mobile phases with a
C18 reversed - phase adsorbent . -
pubmed
Since the
beta - spectrin phosphate sites are located near the C - terminal region and close to the head of the beta - chain that is involved in dimer - dimer interaction , we supposed that the beta - chain phosphorylation has an effect upon the interactions between
spectrin dimers , ie the tetramerization process . -
pubmed
Haemoglobin levels and body
iron stores were measured monthly . -
pubmed
OBJECTIVES : We investigated whether augmentation of
bradykinin ( BK ) bioavailability with
angiotensin - converting enzyme (
ACE ) inhibition is associated with reduced exercise - induced
myocardial ischemia in
hypertension . -
pubmed
Moreover , no correlation was observed between the basal response to
BK or the magnitude of its improvement with EN and with either the dose of
EN or the improvement in exercise ischemic threshold . -
pubmed
CD4(+ ) T lymphocytes participate in protective immunity to ehrlichial pathogens through production of
gamma interferon (
IFN - gamma ) , which promotes switching to high - affinity
immunoglobulin G (
IgG ) and activation of phagocytic cells to produce
nitric oxide . -
pubmed
This study was designed to determine the relative contributions of
MSP1a and
MSP1b proteins , which contain both variant and conserved
amino acid sequences , in stimulating memory
CD4(+ ) T - lymphocyte responses in calves immunized with native
MSP1 . -
pubmed
In the latter case , & gt ; or = 80 % of the
PEN - induced apoptosis was dependent on the presence of wild - type
p53 . -
pubmed
Proinflammatory mediators , reactive
oxygen species , and
TGF - beta can activate pancreatic stellate cells and their synthesis of
collagen I and III . -
pubmed
Interestingly , T - cell responses to all three epitopes are restricted by the
H-2K ( s ) molecule , and this skewed class I restriction may be associated with susceptibility to
demyelinating disease . -
pubmed
Bisphosphorylation of
TnI reduces the affinity of the regulatory site on
troponin C (
TnC ) for
Ca ( 2 + ) by increasing the rate of
Ca ( 2 + ) dissociation . -
pubmed
The ability of phosphorylation of the mutant
TnIs to affect
Ca ( 2 + ) sensitivity has been assessed . -
pubmed
We propose that
TnI residues 16 - 29 bind to
TnC stabilizing the " open "
Ca ( 2 +)-bound state . -
pubmed
Other neuromodulators that can influence intracellular
Ca ( 2 + ) levels include the nucleoside
adenosine , which acts via
A ( 1 ) adenosine receptors subtypes (
A ( 1 ) ARs ) . -
pubmed
Because
A ( 1 ) AR activation inhibits
glutamate release and action ,
A ( 1 ) AR activation may also contribute to
neonatal brain injury . -
pubmed
To examine this possibility , we treated primary neuronal cultures with the
A ( 1 ) AR agonist
CPA , the NMDAR antagonist
MK801 , or
CPA +
MK801 . -
pubmed
These observations show that
A ( 1 ) AR activation and NMDAR blockade lead to early
postnatal cell injury by mechanisms that involve inhibition of intracellular
Ca ( 2 + ) signaling . -
pubmed
We have studied three cell cycle markers in these models :
5 - bromo-2 '- deoxyuridine (
BrdU ) incorporation ( a marker of S phase ) ,
cdc2 protein expression ( a marker of
G2 phase ) , and expression of
MPM2 ( a marker of M phase ) , an epitope phosphorylated by
cdc2 . -
pubmed
We report here that postmitotic dopaminergic neurons undergoing
PCD in the SNpc following
6 - OHDA and axotomy lesions incorporate
BrdU and overexpress
cdc2 , but do not express
MPM2 . -
pubmed
RESULTS : The liposome - plasmid DNA complexes affected metastatic capability in three different ways :
TM - TPS :
DOPE and
DOTAP :
DOPE had no effect on metastatic capability ; a suppressive effect was observed in
DOSPA :
DOPE and
DMRIE :
cholesterol ; while an augmentative effect was observed in
DOTMA :
DOPE and Effectene . -
pubmed
This fact was showed by immunofluorescence analysis which confirmed that
diosgenin strongly enhanced the activation of
p53 in contrast to
hecogenin and
tigogenin actions . -
pubmed
( 99m ) Tc -
ECD SPET gave significantly higher uptake ratio values than
( 99m ) Tc - HMPAO SPET in several symmetrical clusters , including the right and left occipital cuneus , the left occipital and parietal precuneus , and the left superior and middle temporal gyri . -
pubmed
( 99m ) Tc - HMPAO SPET gave significantly higher uptake ratio values than
ECD in two smaller clusters , including the hippocampus in both hemispheres . -
pubmed
In
AD , relative brain uptake of
( 99m ) Tc - HMPAO and
( 99m ) Tc -
ECD is different in several brain regions , some of which are typically involved in
AD , such as the precuneus and the hippocampus . -
pubmed
To analyze the relative sensitivity of the predicted hydrophobic
face of the N - terminal heptad repeat to the insertion of uncharged , polar , and charged substitutions , mutations that introduced
alanine ,
serine , or
glutamic acid into positions 436 , 443 , 450 , and 457 of the
envelope protein were examined . -
pubmed
While no single
amino acid change at any of the positions interfered significantly with the synthesis , processing , or transport to the plasma membrane of
glycoprotein complexes , 9 of the 12 nonconservative mutations in these residues completely abolished fusion activity and virus infectivity . -
pubmed
Mutations in the central positions ( 443 and 450 ) of the heptad repeat region were the most detrimental to
Env function , and even single
alanine substitutions in these positions dramatically altered the fusogenicity of the protein . -
pubmed
When administered locally , the antihyperalgesic effects of
morphine were mediated by peripheral
opioid receptors in both males and females , since this effect was not reversed by i.c.v . -
pubmed
The effect of
gamma globulin and oral
cortisone in the
acute disease . -
pubmed
MAIN OUTCOME MEASURES : The primary endpoint was reduction in urine N - telopeptides of
type 1 collagen (
NTx ) corrected for
creatinine level at 3 months . -
pubmed
RESULTS : Over 3 months ,
ALN produced a significantly greater mean reduction in urine NTx than did
RIS ( -52 % vs -32 % , p & lt ; 0.001 ) , which was maintained at 12 months . -
pubmed
ALN produced a significantly greater mean
BMD increase than did
RIS at 6 months , and it was maintained at 12 months at the lumbar spine ( 4.8 % vs 2.8 % , p & lt ; 0.001 ) and total hip ( 2.7 % vs 0.9 % , p & lt ; 0.001 ) , as well as at the trochanter and femoral neck . -
pubmed
Significant reductions in BSAP with
ALN compared to
RIS were maintained over the 12 months of treatment . -
pubmed
Tolerability was generally similar between
ALN ,
RIS and
PBO , and the incidence of upper
GI AEs causing discontinuation and
oesophageal AEs was similar in the
ALN and
RIS groups . -
pubmed
CONCLUSION : In this study ,
ALN 70 mg OW produced a 50 % greater reduction in
bone resorption as measured by urine NTx and significantly greater increases in lumbar spine and hip
BMD than did
RIS 5 mg daily . -
pubmed
Additional studies are needed comparing OW
ALN with OW
RIS , which became available after the commencement of the present study . -
pubmed
Colchicine decreased the accumulated extracellular matrix and the multiple fibrotic nodules formed in cirrhotic liver , and eliminated
alpha - smooth muscle actin (
alpha - SMA)-positive cells . -
pubmed
In activated stellate cells ,
colchicine inhibited
alpha - SMA and
transforming growth factor - beta1 (
TGFbeta1 ) expression . -
pubmed
Aminoacyl - tRNA ( aa - tRNA ) formation , an essential process in protein biosynthesis , is generally achieved by direct attachment of an
amino acid to tRNA by the
aa - tRNA synthetases . -
pubmed
An exception is
Gln -
tRNA synthesis , which in eukaryotes is catalyzed by
glutaminyl - tRNA synthetase (
GlnRS ) , while most bacteria , archaea , and
chloroplasts employ the transamidation pathway , in which a
tRNA - dependent
glutamate modification generates
Gln - tRNA . -
pubmed
Early work suggested that mitochondria use the transamidation pathway for
Gln -
tRNA formation . -
pubmed
Serum
calcium ,
phosphate ,
parathyroid hormone ,
1,25 - dihydroxyvitamin D ,
bone alkaline phosphatase (
BAP ) ,
osteocalcin , and urine
calcium were measured . -
pubmed
RESULTS :
Alendronate caused significant increase in serum
osteocalcin and
BAP levels biochemically and the number of osteoblasts histopathologically . -
pubmed
Deletion of
orfR , a gene immediately upstream of
orf1 -
orf2 -
omcB that encodes a putative transcriptional regulator , significantly lowered the
omcB transcription when
Fe ( III ) was the electron acceptor and partially inhibited
Fe ( III ) reduction . -
pubmed
From
P8 to
P14 , there was a marked increase in
doublecortin immunoreactive cells in the
AMPA - injected SVZ . -
pubmed
Blockade of the
renin -
angiotensin -
aldosterone - system ( RAAS ) attenuates hepatic
fibrosis . -
pubmed
Serum
laminin (
LN ) and
hyaluronic acid ( HA ) were measured using radioimmunoassays . -
pubmed
Perindopril treatment significantly reduced mean
fibrosis score , protein levels of
AT1R ,
TGF - beta1 and
PDGF - BB , serum levels of HA and
LN , and the activity of
MMP-2,9 . -
pubmed
NF - kappaB DNA binding activity markedly increased in model group ,
perindopril treatment considerably reduced
NF - kappaB DNA binding activity . -
pubmed
FAD oxidizes the
ERO1 - PDI electron transfer chain : the role of membrane integrity . -
pubmed
Microsomal membrane integrity was mandatory for all
FAD - related oxidation steps downstream of
Ero1 . -
pubmed
The
PDI inhibitor
bacitracin could inhibit
FAD - mediated oxidation of microsomal proteins and
PDI , but did not hinder the
FAD - driven oxidation of
Ero1 . -
pubmed
Our data demonstrated that
Ero1 can utilize
FAD as an electron acceptor and that
FAD - driven protein oxidation goes through the
Ero1 -
PDI pathway and requires the integrity of the endoplasmic reticulum membrane . -
pubmed
Our findings prompt further studies to elucidate the membrane - dependent steps of
PDI oxidation and the role of
FAD in redox folding . -
pubmed
The significantly improved therapeutic outcome of animals receiving the triple therapy was attributed to multiple factors , including most likely improved
FMG expression throughout the
tumor and enhanced sensitivity of the
tumor cells to
gemcitabine by adenoviral gene products but also
FMG expression . -
pubmed
HscA and
HscB stimulate [
2Fe-2S ] cluster transfer from
IscU to
apoferredoxin in an
ATP - dependent reaction . -
pubmed
CD spectra indicate that both
HscB and
HscA interact with [
2Fe-2S]
IscU and the rate of cluster transfer was stimulated more than 20 - fold in the presence stoichiometric
HscA and
HscB and excess
MgATP . -
pubmed
No stimulation was observed in the absence of either
HscB or
MgATP , and cluster transfer was found to be an
ATP - dependent reaction based on concomitant
phosphate production and the enhanced rates of cluster transfer in the presence of
KCl which is known to stimulate
HscA ATPase activity . -
pubmed
The results demonstrate a role of the ISC
HscA /
HscB cochaperone system in facilitating efficient [
2Fe-2S ] cluster transfer from the
IscU scaffold protein to acceptor proteins and that [
2Fe-2S ] cluster transfer from
IscU is an
ATP - dependent process . -
pubmed
279 , 53924 - 53931 ] , and mechanistic proposals for coupling of the
HscA ATPase cycle with cluster transfer from [
2Fe-2S]
IscU to
apo - IscFdx are discussed . -
pubmed
Surprisingly , modifications in sustained
K (+ ) currents in
eag and
Shab mutants increased thresholds for DD induction and GF pathway failure . -
pubmed
The search for target analytes to uncover the misuse of long acting
insulin analogues ( Lantus , Insulin Glargine ;
Levemir ,
Insulin Detemir ) in doping control samples led to the identification of several degradation products of
insulin or its synthetic analogues . -
pubmed
In this study , we evaluated in vitro and in vivo the effects of
ZD4054 , an orally active antagonist that specifically binds
ET ( A ) R , as monotherapy , and in combination with
paclitaxel . -
pubmed
ZD4054 treatment also resulted in a reduction of
ET ( A ) R - driven angiogenesis and invasive mediators , such as
vascular endothelial growth factor ,
cyclooxygenase-1 / 2 , and
matrix metalloproteinase (
MMP ) . -
pubmed
The combination of
ZD4054 and
paclitaxel led to the potentiation of all these effects , indicating that
ZD4054 , by blocking the
ET ( A ) R - dependent proliferative , invasive , and antiapoptotic signals , can enhance sensitivity to
paclitaxel . -
pubmed
Furthermore ,
ZD4054 - dependent
tumor growth inhibition was associated with a reduction in proliferation index , microvessel density , and
MMP-2 expression . -
pubmed
Ouabain , a potent inhibitor of the
Na +- K + pump has been shown to reduce fluid absorption in animal models of
PD . -
pubmed
The results of the present study , showing a rapid effect of
ouabain on the RTM of visceral peritoneum , therefore clearly suggest that cell membrane
Na + K +- ATPase is important for peritoneal ionic transport . -
pubmed
Water washed
manganese nodule leached residue ( WMNLR ) calcined at different temperatures was characterized by XRD , FTIR ,
TG - DTA , surface area , surface
oxygen , and surface acid sites . -
pubmed
He was transferred to our center for
F-18 FDG PET /
CT scan under the suspicion of occult
malignancy . -
pubmed
The PET /
CT scan showed increased
FDG uptake in the right cervical
lymphadenopathy and multiple
bony lesions . -
pubmed
After another 6 months of adjusted antituberculosis therapy with
isoniazid ,
rifampin ,
ethambutol , and
clarithromycin , a repeat PET /
CT scan showed remission of previous lesions . -
pubmed
PURPOSE : The role of
arginine vasopressin in
heart failure and the use of
vasopressin receptor antagonists in the treatment of
heart failure are reviewed . -
pubmed
Folding thermodynamics and kinetics of tRNA(
Phe ) , pseudoknots , and mRNA fragments in
DMD simulations are in agreement with previous experimental findings . -
pubmed
( 2 ) The percentage of
CD3-
CD56 + Nk cells in group A ( 44 + /- 5)% and in group B ( 48 + /- 4)% were significantly higher when compared to the control group ( 35 + /- 3)% ( P & lt ; 0.05 ) , there was no significantly difference between the two
mifepristone - treated groups ( P & gt ; 0.05 ) ; The percentage of
CD3-
CD56 +
CD16- subset in group A ( 42 + /- 5)% and in group B ( 45 + /- 5)% were significantly higher when compared to the control group ( 33 + /- 3)% , ( P & lt ; 0.05 ) , there was no significantly difference between the two
mifepristone - treated groups ( P & gt ; 0.05 ) ; the percentages of
CD3-
CD56 +
CD16 + subset in three groups [ ( 2.70 + /- 0.24)% ( 3.26 + /- 0.37)% and ( 2.33 + /- 0.29)% ] were not significantly different ( P & gt ; 0.05 ) . -
pubmed
BACKGROUND :
Magnesium (
Mg ) is an essential cofactor for enzymes involved in
glucose and
insulin metabolism . -
pubmed
The
MS was defined based on criteria set by the Third Report of the National
Cholesterol Education Program except that
body mass index was used in place of waist circumference . -
pubmed
Logistic regression analysis was used to examine the association between quartile categories of
Mg intake , prevalence of
MS and components of the
MS . -
pubmed
RESULTS :
Mg intake was inversely associated with the
MS ; those with the highest intake of
Mg had significantly lower risk of having
MS compared to the lowest quartile of intake ( OR : 0.36 , 95 % CI 0.19 - 0.69 , P for trend 0.002 ) . -
pubmed
Glutathione regulates
integrin alpha ( IIb ) beta(3)-mediated cell adhesion under flow conditions . -
pubmed
Experiments conducted under static conditions using platelet - rich plasma show that platelet activation and adhesion activity of
alpha ( IIb ) beta ( 3 ) are regulated by
glutathione (
GSH -
GSSG ) redox potential . -
pubmed
However , it remains unclear as to whether
GSH - GSSG exerts its regulatory role in platelets by direct targeting of
alpha ( IIb ) beta ( 3 ) or intracellular signals that activate the
integrin . -
pubmed
GSH -
GSSG dose - dependently reduced the number of adherent cells to
fibrinogen and
VWF under 2.5 dyn / cm(2 ) of shear stress , a physical force calculated to be 110 dyne on platelets . -
pubmed
GSH treatment also abolished the hyper - adhesion activity of cells expressing the Pro33 variant of
alpha ( IIb ) beta ( 3 ) . -
pubmed
The results suggest that
GSH may have distinct effects on agonist - induced
alpha ( IIb ) beta ( 3 ) activation and on the
alpha ( IIb ) beta ( 3 )- fibrinogen or
alpha ( IIb ) beta ( 3 )- VWF bonds when exposed to fluid shear stress . -
pubmed
Interaction of
carbon monoxide with the apoptosis - inducing
cytochrome c - cardiolipin complex . -
pubmed
The interaction of
mitochondrial cytochrome ( cyt ) c with
cardiolipin ( CL ) is involved in the initial stages of apoptosis . -
pubmed
This interaction can lead to destabilization of the
heme -
Met80 bond and
peroxidase activity [ Basova , L. V. , et al . -
pubmed
We show that under these conditions
carbon monoxide (
CO ) binds to
cyt c , with very high affinity ( approximately 5 x 10(7 ) M(-1 ) ) , in contrast to the native
cyt c protein involved in respiratory electron shuttling that does not bind
CO . -
pubmed
Binding of
CO to the
cyt c - CL complex inhibits its
peroxidase activity . -
pubmed
Photodissociated
CO from the
cyt c - CL complex shows & lt;20 % picosecond geminate rebinding and predominantly bimolecular rebinding , with a second - order rate constant of approximately 10(7 ) M(-1 ) s(-1 ) , an order of magnitude higher than in
myoglobin . -
pubmed
Together with the findings that ( a ) approximately 30 CL per cyt c are required for full
CO binding and ( b ) salt - induced dissociation indicates that the two negative headgroup charges interact with approximately 5 positive surface charges of the
protein , these results are consistent with a CL anchorage model with an
acyl chain impaled in the
protein [ Kalanxhi , E. , and Wallace , C. J. -
pubmed
The affinity of
CO for the complex is high enough to envisage an antiapoptotic effect of nanomolar
CO concentrations via inhibition of the
cyt c peroxidase activity . -
pubmed
The high - affinity and selective
sigma ( 1 ) antagonists
BD-1063 ,
BD-1047 and
NE-100 , administered subcutaneously ( s.c . ) -
pubmed
The effects of the
sigma ( 1 ) antagonists were reversed by the
sigma ( 1 ) agonist
PRE-084 ( 32 mg / kg , s.c . ) -
pubmed
These results suggest that
sigma ( 1 ) receptors are essential for
capsaicin - induced
mechanical hypersensitivity , but are not involved in mechanical nociceptive
pain . -
pubmed
Compound C caused G(2)/M cell cycle block , accompanied by apoptotic
glioma cell
death characterized by
caspase activation ,
phosphatidylserine exposure and DNA fragmentation . -
pubmed
RESULTS : Among these
GEM sensitivity - related genes ,
dCK alone showed a significant correlation with
GEM efficacy . -
pubmed
CONCLUSION :
dCK mRNA expression is a candidate indicator for
GEM efficacy in unresectable
pancreatic cancer . -
pubmed
The purpose of this study is to identify the hierarchy of importance amongst pathways involved in
fatty acid (
FA ) metabolism and their regulators in the control of hepatic
FA composition . -
pubmed
The resulting model included
FA uptake by the liver , FA oxidation , elongation and desaturation of
FA , which were found active in both genotypes during fasting . -
pubmed
The models showed that
FA were oxidized at a constant and small rate , whereas desaturation of
FA also occurred during fasting . -
pubmed
RESULTS : The
swiprosin-1 augmented
PMA /
A23187 - induced expression of
cytokines and release of
histamine . -
pubmed
However , knock - down of
swiprosin-1 showed only a modest effect on
PMA /
A23187 - induced
cytokine expression , suggesting that
swiprosin-1 has gain - of - function characteristics . -
pubmed
Importantly , either disruption of
actin by
cytochalasin B or inhibition of
PI3 kinase , an enzyme involved in
actin remodeling , by
wortmannin blocked
cytokine expression only in
swiprosin-1 - overexpressing cells . -
pubmed
The
body mass index , weight , waist circumference , fat mass , blood pressure , total
cholesterol , and
low - density lipoprotein cholesterol had decreased significantly . -
pubmed
The correlation analysis showed a positive association between the baseline
visfatin and total
cholesterol level ( r = 0.49 ; P & lt;.01 ) . -
pubmed
We developed fluorescent biosensor systems that are either general or selective to
fluoroquinolone antibiotics by using a single - chain variable - fragment (
scFv ) as a recognition element . -
pubmed
The selectivity of these biosensors to
fluoroquinolone antibiotics was rationally tuned through the structural modification on the pharmacophore of
fluoroquinolone antibiotics and the subsequent selection of
scFv receptor modules against these antibiotics - based antigens using phage display . -
pubmed
A2 is a specific binder for
enrofloxacin and did not cross - react with other
fluoroquinolone antibiotics including structurally similar
ciprofloxacin , while
F9 is a general
fluoroquinolone binder that likely bound to the antigen at the common
pyridone - carboxylic acid pharmacophore . -
pubmed
These
scFv - based receptors were successfully applied to the development of one - step fluorescent biosensor which can detect
fluoroquinolone antibiotics at concentrations below the level suggested in animal drug application guidelines . -
pubmed
The present results verify that in the beta-(1 4)-linkage intramolecular
hydrogen bonding in the aqueous phase is due to the decreased ability of water to successfully compete for the
O5 ' and
HO3 hydrogen bonding moieties , in contrast to that occurring between the
O5 ' and
HO6 atoms in this alpha-(1 4)-linkage . -
pubmed
Phosphatidylinositol 4 - kinases (
PI4Ks ) regulate vesicle - mediated export from the Golgi apparatus via
phosphatidylinositol 4 - phosphate (
PtdIns4P ) binding effector proteins that control vesicle budding reactions and regulate membrane dynamics . -
pubmed
An important hub of this network is
protein kinase D , which regulates the activity of
PI4 K and several
PtdIns4P effectors that control sphingolipid and
sterol content of Golgi membranes . -
pubmed
Other newly identified
PtdIns4P effectors include
Vps74 /
GOLPH3 , a phospholipid flippase called
Drs2 and
Sec2 , a
Rab guanine nucleotide exchange factor (
GEF ) . -
pubmed
IPC - induced increase in decoy receptor expression was reduced by prior treatment with
CAPE , a
nuclear factor - kappa B inhibitor (
NFkappaB ) . -
pubmed
( rac )- 1,1 '- Binaphthyl - based simple
receptors 1 and 2 have been designed , synthesized and studied theoretically . -
pubmed
The
receptors utilize
naphthyridine as the binding motifs for complexation of
dicarboxylic acids in
CHCl ( 3 ) . -
pubmed
The emission of the
BINOL moiety was monitored experimentally to ascertain the selectivity and sensitivity of the
receptors . -
pubmed
Receptor 1 distinguishes
maleic acid from isomeric
fumaric acid by exhibiting different fluorescence behavior and demonstrates stronger binding in the excited state . -
pubmed
The atomistic calculations provide insight into the differential
dicarboxylic acid binding behavior of
receptors 1 and 2 . -
pubmed
MRP1 co - transports its substrate with
glutathione (
GSH ) , leading to lower intracellular
GSH . -
pubmed
In this report we tested whether depleting intracellular
GSH in
MRP1 - expressing cells could hyper - sensitize them to anticancer drugs or not . -
pubmed
We have found that the
GSH contents in
MRP1 - expressing cells are significantly lower than their corresponding control cells . -
pubmed
The treatment with
MRP1 substrate
verapamil or the
GSH synthetase inhibitor
buthionine sulfoxi - mine significantly reduced the intracellular
GSH contents in
MRP1 - expressing cells . -
pubmed
Vitamin D receptor gene polymorphisms and
HLA DRB1 * 04 cosegregation in Saudi
type 2 diabetes patients . -
pubmed
The
vitamin D receptor (
VDR ) gene has been involved in the modulation of susceptibility to inflammatory and
autoimmune conditions , and could play a role in the pathogenesis of
type 2 diabetes mellitus (
T2DM ) . -
pubmed
Genomic DNA was genotyped for the
VDR gene single
nucleotide polymorphisms of
Fok-1 ,
Taq-1 ,
ApaI , and Bsm - I. -
pubmed
Notably , the
VDR polymorphisms observed to be more frequent in
T2DM patients correlated with increased
VDR expression and
IL-12 production , as well as with metabolic parameters of susceptibility to
T2DM , including serum
cholesterol and
high - density lipoprotein levels . -
pubmed
The overall prevalence of
MetS was 19.0 % ( 16/84 ; median treatment duration = 14 months ) in SGA - treated and 0.8 % ( 1/127 ) in SGA - naive children ( OR 29.7 ; 95 % CI 3.85 to 228.40 , P & lt ; 0.001 ) , with an increased prevalence of all components except
high - density lipoprotein cholesterol (
HDL - C ) , respectively : elevated WC ( 40.7 % and 10.1 % ; P & lt ; 0.001 ) ;
hypertriglyceridemia ( 33.7 % and 18.8 % ; P = 0.01 ) ;
impaired fasting glucose ( 12.5 % and 0.7 % ; P = 0.005 ) ; and
elevated blood pressure ( 41.2 % and 16.5 % ; P & lt ; 0.001 ) . -
pubmed
Presence of
abdominal obesity was more sensitive ( 92.9 % ) than
BMI ( 68.8 % ) , while fasting
glucose of 5.6 mmol / L or more and
HDL - C of 1.03 mmol / L or less were most specific ( 94.1 % ) in correctly identifying
MetS. -
pubmed
Hydrogen sulfide decreases the levels of ROS by inhibiting mitochondrial complex
IV and increasing
SOD activities in cardiomyocytes under
ischemia / reperfusion . -
pubmed
We found that
H ( 2 ) S inhibited mitochondrial
complex IV activity and increased the activities of
superoxide dismutases (
SODs ) , including
Mn - SOD and
CuZn - SOD . -
pubmed
Further studies indicated that
H ( 2 ) S up - regulated the expression of
Mn - SOD but not
CuZn - SOD . -
pubmed
Using a cell - free system , we showed that
H ( 2 ) S activates
CuZn - SOD . -
pubmed
An isothermal titration calorimetry ( ITC ) analysis indicated that
H ( 2 ) S directly interacts with
CuZn - SOD . -
pubmed
Taken together ,
H ( 2 ) S inhibits mitochondrial complex
IV and activates
SOD to decrease the levels of ROS in cardiomyocytes during I / R. -
pubmed
AIM : To predict simultaneously the area under the concentration - time curve during one dosing interval [ AUC(0,12 h ) ] for
mycophenolic acid (
MPA ) and
tacrolimus (
TAC ) , when concomitantly used during the first month after transplantation , based on common blood samples . -
pubmed
EXPERT OPINION : Mechanistic studies have discovered that
TSIIA modulates ion channels , kinases ,
hormone receptors , apoptosis proteins , growth factors , cytokines , microRNA ,
tumor suppressor and many other targets , revealing an intricate biological network modulated by
TSIIA . -
pubmed
GC -
MS analysis of circulating
methyl farnesoids during the mid to late 3rd instar showed that
methyl farnesoate is predominant over
methyl epoxyfarnesoate (=
JH III ) . -
pubmed
An equilibrium binding assay confirmed that the mutation ( Q288A ) strongly altered
methyl farnesoate interaction with
USP , while a heterologous cell - line transfection assay confirmed that the mutation did not allosterically alter the transcriptional response of the
ultraspiracle / ecdysone receptor heterodimer to
ecdysteroid signaling . -
pubmed
These results suggest that normal larval to pupal maturation in this mecopteran model insect requires the involvement of a distinct endocrine axis of
USP binding to its own endogenous
terpenoid ligand . -
pubmed
Ceramide 1 - phosphate mediates endothelial cell invasion via the
annexin a2 -
p11 heterotetrameric protein complex . -
pubmed
We have now identified and characterized interactions between
ceramide 1 - phosphate and the
annexin a2 -
p11 heterotetramer constituents . -
pubmed
Although the
annexin a2 -
p11 heterotetramer constituents do not bind the lipid
C-1 - P exclusively , other structurally similar lipids , such as
phosphatidylserine ,
sphingosine 1 - phosphate , and
phosphatidic acid , could not elicit the potent chemotactic stimulation observed with
C-1 - P. -
pubmed
These results imply that extracellular
C-1 - P , acting through the extracellular
annexin a2 -
p11 heterotetrameric protein , can mediate vascular endothelial cell invasion . -
pubmed
Downregulation of miR-133 via
MAPK /
ERK signaling pathway involved in
nicotine - induced cardiomyocyte apoptosis . -
pubmed
Consistently ,
caspase-9 , a target gene for miR-133 , was significantly upregulated , leading to an increase in
caspase-3 , in
nicotine - treated cardiomyocytes compared to non - treated cells . -
pubmed
Lipid peroxidation (
LPO ) and
reduced glutathione (
GSH ) were measured by standard methods . -
pubmed
It was also observed that there was a significant correlation between SIBO with
IL-6 ,
IL-8 ,
TNF - alpha , and
IL-10 ,
LPO and
GSH . -
pubmed
In
stefin B - deficient bone marrow - derived macrophages ( BMDMs ) , we detected an increase in the induction of the LPS - induced pro - inflammatory signal
nitric oxide (
NO ) but decreased
IL-10 expression . -
pubmed
Most importantly ,
lung cancer cells themselves upregulated
IL-6 secretion by activating the
p38 / NF - kappaB pathway through treatment with
cisplatin and
camptothecin . -
pubmed
Juvenile stress reduced the maximum amplitude of the
adrenocorticotropic hormone ( ACTH ) levels ( reduced peak at lights off ) , without affecting the circadian
corticosterone rhythm , but other aspects of the
HPA function ( negative glucocorticoid feedback , responsiveness to further stressors and brain gene expression of
corticotrophin - releasing hormone and
corticosteroid receptors ) remained unaltered . -
pubmed
coli
strains carrying mutations in
PmrAB that confer resistance to
polymyxins , which might have evolved in vivo and have been rarely detected , are described for the first time in enterobacteria isolated from animals . -
pubmed
MLD was then performed on the patterns to deposit organic films predominantly on the
Si surface , with only small amounts growing on the
Cu regions . -
pubmed
A negative potential bias was subsequently applied to the pattern to selectively desorb the layer of SAMs electrochemically from the
Cu surface while preserving the
MLD films on
Si . -
pubmed
P74308 is the most efficient
enzyme for
4 - ONE discovered until now . -
pubmed
The role of the little investigated
carbonyl - reducing enzymes in detoxification seems to be in fact a very old process with rarely observed nonhyperbolic
enzyme kinetics as an adaptation mechanism to higher concentrations of reactive
oxygen species . -
pubmed
Licochalcone A up - regulates of
FasL in mesenchymal stem cells to strengthen bone formation and increase bone mass . -
pubmed
Here we found that 0.35 mg / L
Licochalcone A (
L - A ) had a strong effect in increasing the osteogenic differentiation and mineralization of BMSCs both in vivo and in vitro by up - regulating
FasL and further playing a role in regulating the
ERK and
GSK-3beta - catenin systems . -
pubmed
Results of our study suggested that careful titration of
MSC was response to
L - A and up - regulated
FasL pathways mediating differentiation of
ERK and
GSK-3beta - catenin biological systems under disease state in vivo , restore the impaired function , is one of the ways of
L - A relieve or treatment
osteoporosis . -
pubmed
SHP represents a highly conserved ancient
PTP , and studying
MbSHP should provide a better understanding about the evolution of
tyrosine phosphorylation . -
pubmed
Indeed ,
Delta13Met expression led to a decrease in
HGF - induced
tyrosine phosphorylation of
Met and
ERK phosphorylation , as well as cell proliferation and migration via sequestration of
HGF . -
pubmed
At 6 weeks
post - ACLR , GHK -
Cu groups resulted in a smaller side - to - side difference in
knee laxity as compared to the saline group ( p = 0.009 ) , but there was no significant difference at 12 weeks
post - operation . -
pubmed
The graft complex in the 0.3 mg / ml GHK -
Cu group had higher stiffness than saline group at 6 weeks
post - operation ( p = 0.026 ) , but there was no significant difference in ultimate load , gait parameters , and histological scores among treatment groups . -
pubmed
Body and testis weights , serum
testosterone , testis
malondialdehyde (
MDA ) ,
catalase (
CAT ) ,
superoxide dismutase (
SOD ) ,
glutathione (
GSH ) ,
glutathione S transferase (
GST ) and protein levels were measured , and testis tissue was examined histopathologically and immunohistochemically (
PCNA ) . -
pubmed
MRME treatment rendered significant increases in
body weight , testis weight , testes -
body weight ratio , down regulated the
MDA levels , reduced the degeneration and disruption of seminiferous tubule structure , restored the antioxidant enzymes and serum
testosterone levels , increased the
PCNA activities and attenuated the
testes injury . -
pubmed
A novel oral delivery system that
TPGS modified
docetaxel proniosomes ,
DTX - TPGS -
PN , was developed and the characterization after hydration was observed . -
pubmed
Firstly , Doce - TPGS -
PN was optimized by investing the factors , including the type of surfactant , methods of adding TPGS , content of TPGS and the molar ratio of
span40 /
cholesterol , which may affecting the particle size , encapsulation efficiency and instantaneous release of drug in the formulation . -
pubmed
These results indicate the oral delivery system of
DTX - TPGS -
PN was successfully built with good properties . -
pubmed
Furthermore ,
albumin binds metals and
heme thereby limiting their ability to produce reactive
oxygen and reactive
nitrogen species . -
pubmed
Similar to pathogenic processes that occur during
epilepsy , extravasated
albumin could induce the expression of proinflammatory
cytokines and affect the ability of astrocytes to maintain
potassium homeostasis thereby possibly making neurons more vulnerable to
glutamate exicitotoxicity , which is thought to be a pathogenic mechanism in
MS . -
pubmed
Hypoacetylation of the cells in the presence of
acetyltransferase inhibitor ,
garcinol generally reduced efficiency of BER of
8 - oxoG damage , indicating that some steps in the pathway are stimulated by acetylation . -
pubmed
BACKGROUND : The fibrotic capsule that surrounds
silicone implants consists mainly of
collagen . -
pubmed
( 4 ) The protein expressions of
TGF - beta1 of cells in group BC and 1 , 3 mumol / L
SD-208 groups were respectively 1.00 + -0.08 , 0.80 + -0.08 , and 0.61 + -0.05 , and the protein expressions of
TGF - beta1 of cells in 1 , 3 mumol / L
SD-208 groups were significantly lower than those in group BC ( with t values respectively 4.0 and 9.2 , P values below 0.01 ) . -
pubmed
We quantified urinary
2,4 - D using LC /
MS /
MS . -
pubmed
CD62L test at 2 years of
natalizumab predicts
progressive multifocal leukoencephalopathy . -
pubmed
Exposure to
Cr ( VI ) significantly increased
MDA contents and organ coefficient , whereas decreased
T - SOD activities ,
Ca2 + - ATPase activities , mitochondrial membrane potential and
GSH contents , and histological studies demonstrated
renal damage . -
pubmed
RESULTS : The
ethyl acetate fraction at 200 mg / kg produced significant ( p<0.05 ) elevations of
catalase enzyme activity and a significant ( p<0.05 ) reduction in serum
malondialdehyde . -
pubmed
Furthermore ,
17beta - estradiol downregulated the expression of
intercellular adhesion molecule-1 ( P = .0001 ) and
P - selectin ( P & lt ; .0001 ) on the endothelium and increased the protein expression of
eNOS ( P & lt ; .0001 ) . -
pubmed
CONCLUSIONS : The prophylactic treatment with
17beta - estradiol showed better overall repercussions and was able to prevent any fatal occurrence , increase
eNOS expression , thus preserving mesenteric perfusion and intestinal integrity , and reduce
inflammation . -
pubmed
Exposing
SCLC lines to
tubulin - targeted agents (
paclitaxel or
vinorelbine ) simultaneously with
etoposide /
carboplatin resulted primarily in less than additive cell killing . -
pubmed
As single agents ,
nuclear kinase inhibitors including
Aurora kinase inhibitors ,
Kinesin Spindle Protein /
EG5 inhibitors , and
Polo - like kinase-1 inhibitors were potent cytotoxic agents in
SCLC lines ; however , simultaneous exposure of the
SCLC lines to these agents along with
etoposide /
carboplatin , generally , resulted in less than additive cell killing . -
pubmed
Exposure of the
SCLC lines to the
MDM2 inhibitor
JNJ-27291199 produced enhanced killing in 80 % of the
SCLC lines . -
pubmed
Chk-1 inhibitors such as
rabusertib increased the
cytotoxicity of
etoposide /
carboplatin to the
SCLC lines in an additive to greater than additive manner . -
pubmed
The combination of
GSK-3beta inhibitor
LY-2090314 with
etoposide /
carboplatin increased killing in approximately 40 % of the
SCLC lines . -
pubmed
Exposure to the
BET bromodomain inhibitor
MK-8628 increased the
SCLC cell killing by
etoposide /
carboplatin in 20 - 25 % of the
SCLC lines . -
pubmed
Only 10 - 15 % of the
SCLC lines had an increased response to
etoposide /
carboplatin when simultaneously exposed to the
PARP inhibitor
talazoparib . -
pubmed
Efficacy research of
salazosulfamide in
ankylosing spondylitis and
NAT1 gene polymorphism . -
pubmed
The aim of this study was to explore the correlation of
salazosulfamide efficacy on
ankylosing spondylitis and
N - acetyltransferase 1 (
NAT1 ) gene polymorphism . -
pubmed
Overall ,
salazosulfamide demonstrates curative activity for
ankylosing spondylitis and we believe that
NAT1 AA / GG genotype at 263 locus can promote
salazosulfamide effectiveness on
ankylosing spondylitis . -
pubmed
Serum parathyroid hormone and
alkaline phosphatase as predictors of
calcium requirements after total parathyroidectomy for
hypocalcemia in
secondary hyperparathyroidism . -
pubmed
RESULTS : There is a significant correlation between the postoperative amount of
calcium requirement and preoperative
serum alkaline phosphatase level ( r = 0.442 ; P & lt ; .001 ) and
parathyroid hormone level (
PTH ; r = 0.889 ; P & lt ; .001 ) . -
pubmed
For prediction of insufficient
calcium supplement , the cutoff point of
PTH and
alkaline phosphatase level values were calculated by receiver operating characteristic ( ROC ) curve analysis and modified equations were developed by regression analysis . -
pubmed
Therefore , we investigated whether concomitant inhibition of the two main antioxidant defense pathways , that is , the
thioredoxin (
TRX ) and the
glutathione (
GSH ) systems , presents a new strategy to trigger cell
death in
RMS . -
pubmed
gamma - glutamylcysteine synthetase inhibitor ,
buthionine sulfoximine (
BSO ) or the
cystine / glutamate antiporter inhibitor
erastin (
ERA ) , synergize with
thioredoxin reductase (
TrxR ) inhibitor
auranofin (
AUR ) to induce cell
death in RMS cells . -
pubmed
Interestingly ,
AUR causes accumulation of ubiquitinated proteins when combined with
BSO or
ERA , in line with recent reports showing that
AUR inhibits the proteasome besides
TrxR. -
pubmed
Consistently ,
AUR /
BSO or
AUR /
ERA cotreatment increases ubiquitination and expression of the short - lived proteins
NOXA and
MCL-1 , accompanied by increased binding of
NOXA to
MCL-1 . -
pubmed
Notably ,
NOXA knockdown significantly rescues RMS cells from
AUR /
BSO- or
AUR /
ERA - induced cell
death . -
pubmed
In addition ,
AUR acts together with
BSO or
ERA to stimulate
BAX /
BAK and
caspase activation . -
pubmed
Importantly , re - supply of
GSH or its precursor
NAC completely prevents
AUR /
ERA- and
AUR /
BSO - induced accumulation of ubiquitinated proteins ,
NOXA upregulation and cell
death , indicating that
GSH depletion rather than ROS production is critical for
AUR /
BSO- or
AUR /
ERA - mediated cell
death . -
pubmed
M344 promotes nonamyloidogenic
amyloid precursor protein processing while normalizing
Alzheimer ' s disease genes and improving memory . -
pubmed
We show that the small - molecule
histone deacetylase inhibitor
M344 reduces
beta - amyloid (
Abeta ) , reduces
tau Ser396 phosphorylation , and decreases both
beta - secretase (
BACE ) and
APOEepsilon4 gene expression . -
pubmed
M344 increases the expression of
AD - relevant genes :
BDNF ,
alpha - secretase (
ADAM10 ) ,
MINT2 ,
FE65 ,
REST ,
SIRT1 ,
BIN1 , and
ABCA7 , among others . -
pubmed
M344 increases
sAPPalpha and
CTFalpha APP metabolite production , both cleavage products of
ADAM10 , concordant with increased
ADAM10 gene expression . -
pubmed
M344 also increases levels of immature
APP , supporting an effect on
APP trafficking , concurrent with the observed increase in
MINT2 and
FE65 , both shown to increase immature
APP in the early secretory pathway . -
pubmed
The roles of RNA
5 - methylcytosine ( RNA : m5C ) and RNA : m5C methyltransferases (
RCMTs ) in lineage - associated chromatin organization and drug response / resistance are unclear . -
pubmed
hnRNPK interacts with the
lineage - determining transcription factors ( TFs ) ,
GATA1 and
SPI1 / PU.1 , and with
CDK9 /
P - TEFb to recruit
RNA - polymerase - II at nascent RNA , leading to formation of
5 - Azacitidine (
5 - AZA)-sensitive chromatin structure . -
pubmed
In contrast ,
NSUN1 binds
BRD4 and
RNA - polymerase - II to form an active chromatin structure that is insensitive to
5 - AZA , but hypersensitive to the
BRD4 inhibitor
JQ1 and to the downregulation of
NSUN1 by siRNAs . -
pubmed
This study reveals novel RNA : m5C /
RCMT - mediated chromatin structures that modulate
5 - AZA response / resistance in
leukaemia cells , and hence provides a new insight into treatment of
leukaemia . -
pubmed
By probing ( Mo2/3 M1/32)2 AC ( where M2 = Sc , Y and A =
Al ,
Ga ,
In ,
Si ,
Ge ,
In ) , we predict seven stable i -
MAX phases , five of which should have a retained stability at high temperatures . -
pubmed
Furthermore , based on i -
MAX phases as parent materials for 2D
MXenes , we also expect that the range of
MXene compositions will be expanded . -
pubmed
RESULTS :
CCl4 administration increased liver weight , decreased
body weight , elevated plasma
alanine aminotransferase , and
aspartate aminotransferase and increased liver oxidative stress (
malondialdehyde and
glutathione ) . -
pubmed
Moreover ,
SE inhibited
CCl4 - induced nuclear translocation of hepatic
nuclear factor kappa B (
NF - kappaB )
p65 and phosphorylation of
mitogen - activated protein kinase (
MAPK ) . -
pubmed
CONCLUSION : These results suggested that
SE prevented
CCl4 - induced
hepatic fibrosis by inhibiting the
MAPK and
NF - kappaB signaling pathways . -
pubmed
Formaldehyde (
FA ) is a ubiquitous environmental pollutant , which can induce apoptosis in lung cell and is related to the pathogenesis of
asthma ,
pneumonia , and
chronic obstructive pulmonary disease . -
pubmed
Heat shock protein 70 (
Hsp70 ) is an
ATP - dependent molecular chaperone and exhibits an anti - apoptosis ability in a variety of cells . -
pubmed
Inhibitor (
LY 294002 , a specific inhibitor of
PI3K -
Akt ) test result indicated that
PI3K -
Akt signaling pathway was involved in the inhibition of FA - induced apoptosis by
Hsp70 overexpression and also active in the maintenance of
GLI2 level . -
pubmed
The influence of different ratios of starch /
polyvinyl alcohol (
PVA)/
citric acid and different drying times on the performance properties , transparency , tensile strength (
TS ) , water vapor permeability ( WVP ) , water solubility (
WS ) , color difference ( DeltaE ) , and antimicrobial activity of the ternary blends films were investigated . -
pubmed
We found that
niclosamide - induced endosomal neutralization prevented
E glycoprotein conformational changes on the virion surface of flaviviruses , resulting in the release of non - infectious immature virus particles with uncleaved pr peptide from host cells . -
pubmed
Kidney tissues were harvested at 8 weeks
post -
STZ and analyzed by a hydrophilic interaction liquid chromatography ( HILIC ) ultra - performance liquid chromatography - quadrupole time - of flight mass spectrometry ( UPLC - QTOF
MS ) . -
pubmed
Meprin expression - associated metabolites with strong variable importance of projection ( VIP ) scores were
indoxyl sulfate ,
Ng - L - Glutamyl - L - aspartic acid ,
N - Methyl-4 - pyridone-3 - carboxamide ,
inosine , and
cis-5 - decenedioic acid . -
pubmed
Meprin deficiency - associated differentiating metabolites with high
VIP scores were
cortisol ,
hydroxymethoxyphenylcarboxylic acid - O - sulphate , and
isovaleryalanine . -
pubmed
The data suggest that
meprin beta activity enhances
diabetic kidney injury in part by altering the metabolite balance in kidneys , favoring high levels of uremic toxins such as
indoxyl sulfate and
N - Methyl - pyridone - carboxamide . -
pubmed
The results showed that serum protein profiles were linearly significantly increased of serum
phosphor ,
uric acid ,
aspirate aminotransferase ,
alanine transaminase and
alkaline phosphatase in groups treated with Roundup , as well as the serum concentrations of
triglyceride altered after treatment with
glyphosate . -
pubmed
MALDI -
TOF mass spectrometry and NMR spectroscopy revealed the molecular weight of the
acetyl - xylogalactan to be 88.5 kDa . -
pubmed
Versatile derivatization for
GC -
MS and LC -
MS : alkylation with
trialkyloxonium tetrafluoroborates for inorganic anions , chemical warfare agent degradation products , organic acids , and proteomic analysis . -
pubmed
Recent analytical applications of
trialkyloxonium derivatizations include the high - precision determination of inorganic anions in complex matrices , the qualitative confirmation of chemical warfare agent degradation products in soils , the profiling of
carboxylic acids in urine , and the detection of
protein post - translational modifications induced by
carbon dioxide . -
pubmed
Graphical Abstract Alkylation with
trialkyloxonium salts for
GC -
MS and LC -
MS analysis . -
pubmed
In the case when the fraction of
TBCHA is equal or higher than the fraction of
VCL , the microgels become almost insensitive to the temperature changes , and the amount of water inside the microgels appeared to be diminishingly small . -
pubmed
Phenobarbital , a well - known
liver cancer promoter , has been found to promote hepatocyte proliferation via
CAR activation . -
pubmed
Unigene33312 and Unigene33024 were found to be the most stably expressed genes under salt stress and
Cd stress . -
pubmed
Moreover ,
Unigene33024 and
PP2A were the most suitable reference genes under
Cr stress and Unigene33024 and Sb09g019750 were deemed more suitable reference genes under
As stress . -
pubmed
The expression patterns of two target genes (
Cu /
Zn SOD and
CAT ) were used to further verify those selected reference genes under different conditions . -
pubmed
In the absence of measured
rivaroxaban exposure , exposure estimates were predicted based on individual increases in
prothrombin time ( PT ) and the known correlation between
rivaroxaban plasma concentrations and PT dynamics . -
pubmed
Predicted
rivaroxaban exposure and CrCl were significantly associated with both efficacy outcomes in the
BID period . -
pubmed
AIM : To compare morbidity ,
postoperative complications , and early survival between liver grafts perfused with IGL-1 and those perfused with
histidine - tryptophan - ketoglutarate (
HTK ) solutions . -
pubmed
DESIGN : In this cross - sectional study , participants completed abdominal MRI to measure hepatic fat fraction ( HFF ) , oral
glucose tolerance test , and
PNPLA3 rs738409 genotyping . -
pubmed
All the viral proteins were synthesized in the presence of TM , but the
glycoproteins were selectively altered in that they migrated faster than normal viral
glycoproteins when analyzed by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis , suggesting defective glycosylation . -
pubmed
Within 1 h after
TM addition ,
[ 14C ] glucosamine incorporation into
glycoproteins was inhibited 20 % , whereas
[ 35S ] methionine incorporation was unaffected . -
pubmed
By 2 to 3 h after
TM addition ,
glucosamine incorporation had fallen to 15 % of control value , with
methionine incorporation being 60 % of normal . -
pubmed
A simple test system for the separation of staphylococci from micrococci is described , which is based on the ability of staphylococci to produce acid aerobically from
glycerol in the presence of 0.4 mug of
erythromycin per ml and on their sensitivity to
lysostaphin . -
pubmed
Very high levels of urinary
catecholamines suggested a diagnosis of
pheochromocytoma , but no
tumour was found at computerized tomography (
CT ) and
metaiodobenzylguanidine (
MIBG ) scintigraphy . -
pubmed
No
tumour was visible at
CT or ultrasonography and another
MIBG scintigraphy proved negative . -
pubmed
These data are consistent with the hypothesis that an increase in the release of
catecholamines within the caudal PVN in response to
hemorrhage is facilitatory for the release of
adrenocorticotropin and/or
vasopressin . -
pubmed
Multiple short - echo ( 2.5 - ms ) quantitation of in vivo
sodium T2 relaxation . -
pubmed
Early studies on excised tissue samples revealed that the
sodium nucleus exhibited a two - component
T2 relaxation . -
pubmed
Inhibition of cytotoxic T lymphocyte - mediated lysis and cellular proliferation by
isoquinoline sulfonamide protein kinase inhibitors . -
pubmed
In addition , suggestive evidence is provided that
protein kinase C , or some other enzyme with similar sensitivity to the
isoquinoline sulfonamides , plays an important role in cellular proliferation . -
pubmed
It is suggested that , as in
sarcoidosis ,
ACE levels are useful in the diagnosis , prognosis and follow - up of
Crohn ' s disease , especially in relation to the use of
steroid drugs . -
pubmed
The effect of mAbs on the
alpha BgTx and cholinergic ligand binding properties of the
AcChR molecule defined three major categories of mAbs : those that block
alpha BgTx and
carbamylcholine ( agonist ) binding , but do not block
d - tubocurarine ( antagonist ) binding ( 383C , 572C , 370C and 249E ) ; mAb 387D , which blocks agonist binding and partially blocks alpha BgTx and
d - tubocurarine binding ; and
mAb 247 G , which does not affect agonist binding , blocks at most 50 % of the
alpha BgTx binding sites , and decreases the affinity of the high affinity component of
d - tubocurarine binding ( Mihovilovic , M. , and Richman , D. P. ( 1984 ) J. Biol . -
pubmed
They also indicate the nonequivalence of the two alpha - toxin binding regions of the
AcChR molecule and suggest that it is possible to identify epitopes within the
alpha BgTx binding region that when bound produce differential effects on the binding of the agonist (
carbamylcholine ) and the antagonist (
d - tubocurarine ) . -
pubmed
Comparison of the effects of
apamin , a
Ca2 + - dependent K + channel blocker , and
arylazido aminopropionyl ATP (
ANAPP3 ) , a
P2 - purinergic receptor antagonist , in the guinea - pig vas deferens . -
pubmed
These studies provide additional support for the view that
ANAPP3 antagonizes
ATP - induced responses of the guinea - pig vas deferens by blocking
P2 - purinergic receptors . -
pubmed
By using the immunoadsorbent columns of 6A4 antibody and
anti - I - Ab ,
TaF , in a manner similar to
TsF , was demonstrated to be composed of two chains , i.e. -
pubmed
Noradrenaline ( 1 microM , in the presence of the uptake inhibitor
desipramine ) inhibited
K +-induced
[ 3H ] NA release by activation of presynaptic
alpha - receptors . -
pubmed
When the
Ca2 + -concentration in the medium was reduced , or the
Mg2 + -concentration increased ,
[ 3H ] NA release appeared to be more susceptible to
alpha - receptor mediated inhibition . -
pubmed
Veratrine - induced release was also inhibited by
alpha - receptor activation . -
pubmed
However ,
[ 3H ] NA release induced by the
calcium - ionophore was not affected by
alpha - receptor agonists . -
pubmed
These results strongly support the view that
alpha - receptor activation results in a decrease of the availability of
Ca2 + for stimulus - secretion coupling processes . -
pubmed
The essential characteristics of pHSG415 and pHSG422 may be summarized as follows : ( 1 ) their genome copy number is low ( 4 - -6 copies / chromosome ) ; ( 2 ) their replication ceases at high temperature and they are rapidly lost from host cells grown at temperatures of 37 degrees C and above ; ( 3 ) the relaxation nick site of pSC101 , which is thought to be synonymous with its origin of transfer replication , is absent from the vectors ; as a consequence , they are not mobilized to a significant extent by co - existing conjugative plasmids that are able to mobilize wild - type pSC101 ; ( 4 ) they contain unique insertion sites for DNA fragments generated by the following
restriction endonucleases :
EcoRI , XhoI , XmaI ,
HindIII and PstI ; pHSG415 additionally contains single
BamHI ,
BstEII and
HincII sites and may also be used to clone PvuI - generated fragments ; ( 5 ) the plasmids confer upon their host cells resistance to
chloramphenicol ,
kanamycin and
ampicillin , and every unique cloning site , except those of
BamHI and BstEII , is located within one of these antibiotic - resistance genes . -
pubmed
This elevated intracellular
calcium concentration is responsible for cytoskeletal modifications which alter cell shape , the activation of
phospholipases which results in perpetuation of membrane damage and finally ,
mitochondrial calcification . -
pubmed
These effects of
arachidonate are specific to these cations , concentration - dependent , and sensitive to
lipoxygenase inhibitors . -
pubmed
Arachidonic acid - dependent , but not
F - Met - Leu - Phe - dependent ,
calcium release is sensitive to
lipoxygenase inhibitors . -
pubmed
Alcohol and Serum
creatinine kinase levels . -
pubmed
The remaining relaxation elicited by
acetylcholine could be blocked with 30 mmol / l
KCl or with 10 mumol / l
ouabain ( inhibitor of
Na + , K (+)- ATPase ) , and , when combined with
NG - nitro - L - arginine methyl ester , these interventions abolished
acetylcholine - induced relaxation . -
pubmed
Tolbutamide at 2 mmol / l or 10 mumol / l
glyburide ( blockers of
ATP - sensitive potassium channels ) partially inhibited
NG - nitro - L - arginine methyl ester - resistant endothelium - dependent relaxation. -
pubmed
Theophylline ( 3 x 10(-5)M ) and the
phosphodiesterase inhibitor
AH 21 - 132 ( 3 x 10(-5)M ) did not alter the enhancement of
3H - overflow caused by either
cyclic AMP or
adenosine . -
pubmed
Forskolin ( 3 x 10(-6)M ) and the
phosphodiesterase inhibitors
ICI 63 197 ( 10(-4)M ) and
AH 21 - 132 ( 3 x 10(-6)-3 x 10(-5)M ) increased stimulation - evoked
3H - overflow . -
pubmed
The purified
rPhl p V induced specific
histamine release of blood basophils from grass pollen -
allergic patients . -
pubmed
O6 - Benzyl-2 '- deoxyguanosine is a potential antitumor drug modulator that is intended to reduce or eliminate
O6 - alkylguanine - DNA alkyltransferase activity in
tumors prior to treatment with genotoxic chemotherapeutic alkylating agents . -
pubmed
Analysis of venous blood showed the presence of
O6 - benzyl-2 '- deoxyguanosine and
O6 - benzylguanine at concentrations which are considered to be effective in depleting
alkyltransferase activity . -
pubmed
The effect of recombinant
insulin - like growth factor I on
ketone body , lipid and
apolipoprotein concentrations and its use to treat
ketoacidosis in severe
insulin resistance . -
pubmed
One month later , the effect of 20 mg
rhlGF - I infused daily for 4 days on
ketone body concentrations was studied . -
pubmed
Further studies of
rhlGF-1 use caused a fall in concentrations of
cholesterol ,
triglyceride ,
VLDL ,
LDL , and
apolipoprotein B. -
pubmed
The Sp isomer of
6 - thioethylpurineriboside 3',
5 '- phosphorothioate (
6SEth - cPuMPS ) does not bind to the surface receptor , enters the cell and has relative high affinity for
protein kinase A. -
pubmed
These results indicate that intracellular
cAMP formation and activation of
protein kinase A are essential for down - regulation of the surface
cAMP receptor . -
pubmed
pylori did not differ from those with negative serology with respect to age , duration of
acromegaly , duration of
octreotide treatment or
serum GH level . -
pubmed
Cell morphology , protein concentrations ,
lactate dehydrogenase leakage , energy metabolism , as reflected by specific transport and incorporation of
2 - D -[ 3H ] deoxyglucose , as well as
superoxide radical formation were analyzed as a function of time . -
pubmed
In this paper we present a new method for the detection of resistance to
activated protein C (
APC ) that is based on direct measurement of the effect of
APC on the cofactor activity of
plasma factor Va. -
pubmed
The
factor V present in a diluted plasma sample was activated with
thrombin and its sensitivity towards
APC was subsequently determined by incubation with phospholipids and
APC . -
pubmed
Addition of 0.2 nM
APC and 20 microM
phospholipid vesicles to a 1000 - fold diluted sample of
thrombin - activated normal plasma resulted in loss of more than 85 % of the cofactor activity
factor Va within 6 min . -
pubmed
The
APC - sr was defined as the ratio of the
factor Va cofactor activities determined in
thrombin - activated plasma samples after 6 min incubation with or without 0.2 nM
APC and was multiplied by 100 to obtain integers (
APC - sr = ? -
pubmed
Here , we analyzed the role of free
calcium signaling in the induction of
CTF-1 transcriptional activity by
TGF - beta . -
pubmed
TGF - beta induction of
CTF-1 is inhibited in cells pretreated with
thapsigargin , which depletes the endoplasmic reticulum
calcium stores , thus further arguing for the potential relevance of
calcium mobilization in
TGF - beta action . -
pubmed
The immunosuppressants
cyclosporin A and
FK506 abolish
calcineurin - mediated induction of
CTF-1 activity . -
pubmed
However ,
TGF - beta still induces the
CTF-1 TAD in cells treated with these compounds or in cells overexpressing both
calcineurin and
DeltaCaMKIV , suggesting that other
calcium - sensitive enzymes might mediate
TGF - beta action . -
pubmed
These results identify
CTF /
NF - I as a novel
calcium signaling pathway - responsive
transcription factor and further suggest multiple molecular mechanisms for the induction of
CTF /
NF - I transcriptional activity by
growth factors . -
pubmed
Serotonin is strongly related to both types of memory , and particularly , to
STM , however , its regulatory role is still unclear . -
pubmed
In this study , the effects of
tryptophan (
TRY ) restriction on learning and
STM were evaluated . -
pubmed
This decline in
insulin - mediated
glucose uptake was accounted for by a decrease in whole body glycolytic rate with no change in the rate of glycogen synthesis . -
pubmed
A hydrophobic loop on the surface of
SEB and other
bacterial superantigens , centered around a conserved
leucine ( L45 ) residue , is essential for binding to
class II major histocompatibility complex molecules . -
pubmed
Vaccines against
staphylococcal superantigens should focus on this critical
leucine residue . -
pubmed
RESULTS :
PACAP27 produced dose dependent relaxations of 10 microM rings preconstricted with
prostaglandin F2 alpha (
PGF2 alpha ) with half maximal relaxation ( IC50 ) at 17 nM. -
pubmed
Pretreatment of the vessels with the
ATP sensitive K+ ( KATP ) channel blocker
glibenclamide ( 1 microM ) or with the
Ca2 + activated K + ( KCa ) channel blocker
iberiotoxin ( 100 nM ) inhibited the
PACAP27 induced relaxation . -
pubmed
In response to decreased
oxygen delivery / metabolic " distress " , the
RPE may elaborate substances leading to CNV growth . -
pubmed
First observation of the
EL2 lattice defect in
indium gallium arsenide grown by molecular - beam epitaxy . -
pubmed
Functionally , the
oatp2 - expressing oocytes were found to transport
dehydroepiandrosterone sulfate ,
delta1 opioid receptor agonist [
D - Pen2,D - Pen5 ] enkephalin ,
Leuenkephalin , and
biotin significantly , as well as the substrates previously reported . -
pubmed
We have studied the substrate specificity of a recombinant
Sgs1 helicase (
amino acid residues 400 - 1268 of the
Sgs1 protein ) . -
pubmed
DNase I and
hydroxyl radical footprinting of the
Sgs1 - DNA complex shows that the protein binds specifically to the junction of a double - stranded DNA and its 3 ' overhang . -
pubmed
The role played by
CAMs and other membrane - associated molecules is discussed in the sense that they are either inductor
molecules that turn on different
homeobox genes , or downstream products of genes , or both . -
pubmed
Kinetics study of a
selenium - containing
ScFv catalytic antibody that mimics
glutathione peroxidase . -
pubmed
The steady - state kinetics study and some enzymatic characterization of a
selenium - containing
scFv catalytic antibody (
Se - scFv2F3 ) were carried out . -
pubmed
The obtained kinetics parameters from the kinetics study of
Se - scFv2F3 were analyzed and compared with those of native
glutathione peroxidase . -
pubmed
The parameters measured were : serum levels of
albumin (
ALB ) ,
nitrate ,
glucose ,
insulin ,
corticosterone (
CORT ) ,
prolactin (
PRL ) and
PRL mRNA in the adenopituitaries . -
pubmed
Arthritis increased serum
NO and reduced
ALB levels ; both changes were significantly restored in the
FR - AA group . -
pubmed
Significant
Gal9p - mediated transport could be demonstrated for
[ ( 14 ) C ]- uric acid and for
[ ( 14 ) C ]- PAH(1 ) . -
pubmed
The data of the present study sustain the idea of the involvement of
galectin 9 in immune /
inflammation processes and in potential - sensitive
uric acid translocation . -
pubmed
Chromosomal integration of
retinoic acid response elements prevents cooperative transcriptional activation by
retinoic acid receptor and
retinoid X receptor . -
pubmed
All - trans - retinoic acid receptors (
RAR ) and
9 - cis - retinoic acid receptors (
RXR ) are nuclear receptors known to cooperatively activate transcription from
retinoid - regulated promoters . -
pubmed
The effect of
15d - PGJ ( 2 ) on
MMP-1 promoter activation was analysed in transient transfection experiments , while electrophoretic mobility shift assays were performed to study the binding activity of the
transcription factor AP-1 . -
pubmed
PPARgamma activators (
15d - PGJ ( 2 ) and BRL 49653 ) inhibited
IL-1beta - induced
MMP-1 synthesis in a dose - dependent manner . -
pubmed
Interestingly ,
15d - PGJ ( 2 ) reduced both basal and
IL-1beta - induced
AP-1 binding activity . -
pubmed
The reduced pressure response to
vasopressin during acute
sepsis has directed our interest to the regulation of
vasopressin V ( 1A ) receptors . -
pubmed
V ( 1A ) receptor expression was downregulated by the
cytokines in a
nitric oxide - independent manner . -
pubmed
Blood pressure dose - response studies after injection of endotoxin showed a diminished responsiveness to the selective
V ( 1 ) receptor agonist
Phe(2),
Ile(3),
Orn ( 8 )- vasopressin . -
pubmed
We propose that this downregulation of
V ( 1A ) receptors contributes to the attenuated responsiveness of blood pressure in response to
vasopressin and , therefore , contributes to the
circulatory failure in
septic shock . -
pubmed
In contrast , we found that fasting is associated with increase in the activity of
acidic endoglycosidases ( of lysosomal origin ) which degraded
hyaluronic acid ,
chondroitin-4 - sulfate ,
chondroitin-6 - sulfate and heparin . -
pubmed
Increased
[ 3H ] thymidine incorporation persisted for three days following treatment with a single dose of
GLP-2 . -
pubmed
The recombinant
strain expressed more than 700 - fold of the
beta - gal activity as compared to the wild type
strain under induction by
maltose . -
pubmed
Chloride transport across vesicle and cell membranes by
steroid - based receptors . -
pubmed
The most significant changes were seen in the
MIF +
MPA group . -
pubmed
In the
MPA group , the pre - treatment and
post - treatment expression of
PR ( 2.9 + /- 1.1 , 1.6 + /- 0.8 ) ,
ER ( 2.8 + /- 0.9 , 1.4 + /- 0.9 ) ,
PCNA ( 0.84 + /- 0.10 , 0.60 + /- 0.12 ) ,
bcl-2 ( 0.236 + /- 0.089 , 0.157 + /- 0.981 ) and CD(44v6 ) ( 4.6 + /- 1.8 , 2.5 + /- 1.9 ) were all decreased ( all P & lt ; 0.01 ) ; the expression of
bax ( 0.20 + /- 0.10 , 0.42 + /- 0.07 ) was increased ( P & lt ; 0.01 ) . -
pubmed
In the
MIF +
MPA group , the expression of
PR ( 3.2 + /- 1.0 , 0.8 + /- 0.8 ) ,
ER ( 2.7 + /- 0.9 , 0.7 + /- 0.9 ) ,
PCNA ( 0.81 + /- 0.09 , 0.25 + /- 0.09 ) ,
bcl-2 ( 0.225 + /- 0.091 , 0.066 + /- 0.009 ) and
CD ( 44v6 ) ( 4.5 + /- 1.9 , 2.7 + /- 1.6 ) were all decreased ( all P & lt ; 0.01 ) ; the expression of
bax ( 0.22 + /- 0.06 , 0.59 + /- 0.09 ) was increased ( P & lt ; 0.01 ) ; there were significant different expression of
PCNA ,
ER ,
PR ,
bax and
bcl-2 as compared with the
MIF group and the
MPA group , respectively ( all P & lt ; 0.01 ) . -
pubmed
The expression of CD(44v6 ) was significantly different ( P & lt ; 0.01 ) between the
MIF +
MPA group , and the
MIF group , but not significantly different between the
MIF +
MPA group and the
MPA group . -
pubmed
CONCLUSIONS : The study indicates that high dose
progesterone could inhibit the growth , promote apoptosis and inhibit
metastasis of
endometrial carcinoma ,
MIF could inhibit the growth and promote apoptosis ,
MIF +
MPA could more strongly inhibit the growth , promote apoptosis and inhibit
metastasis of
endometrial carcinoma than
MIF or
MPA , and synergistic effect was observed on the expression of
PCNA ,
ER ,
PR ,
bax and
bcl-2 . -
pubmed
Changes in mRNA expression of gene targets (
estrogen responsiveness , cell cycle progression , apoptosis and
aromatase activity ) in
tumors induced by the different diets were evaluated . -
pubmed
The effect of
EPI is prevented by
beta ( 2 )- AR blockade and is associated with an increase in
G protein - coupled receptor kinase 3 (
GRK3 ) expression . -
pubmed
Furthermore , 24 - h
EPI or NE treatments that produced desensitization also resulted in a selective 2 - fold up - regulation of
GRK3 ;
GRK2 was unchanged . -
pubmed
The
beta - AR antagonist
alprenolol ( 1 microM ) and
GRK3 antisense ( but not sense ) DNA blocked 0.3 microM
EPI- and 10 microM NE - induced desensitization and down - regulation of the
alpha ( 2B )- AR as well as
GRK3 up - regulation . -
pubmed
In conclusion , simultaneous activation of
alpha ( 2B )- and beta ( 2 )- ARs results in a 67 - fold decrease in the threshold concentration of
EPI required for
alpha ( 2B )- AR down - regulation . -
pubmed
Backbone residual dipolar coupling (
N - H , Calpha - Halpha ,
N - C ' , and Calpha - C ' ) data collected in five different media on the
B3 IgG binding domain of
streptococcal protein G ( GB3 ) have been analyzed by simultaneous refinement of the coordinates and optimization of the magnitudes and orientations of the alignment tensors using single and multiple structure representations . -
pubmed
BACKGROUND :
S100B , a 20 - kDa ,
Ca2 + -binding dimer , is a putative intrinsic negative regulator of
myocardial hypertrophy expressed after
myocardial infarction . -
pubmed
AIM :
Thiazolidinediones (
TZD ) are a new class of oral antidiabetic drugs that have been shown to inhibit growth of same epithelial
cancer cells . -
pubmed
Although
TZD were found to be ligands for
peroxisome proliferator - activated receptor gamma (
PPARgamma ) , the mechanism by which
TZD exert their anticancer effect is presently unclear . -
pubmed
RESULTS : Exposure to
TZD inhibited colony formation in a
PPARgamma - dependent manner . -
pubmed
CONCLUSION :
TZD treatment in
pancreatic cancer cells has potent inhibitory effects on growth by a
PPAR - dependent induction of pancreatic ductal differentiation . -
pubmed
Besides the essential nutrients , non - essential food constituents such as non - digestible
carbohydrates may also have an impact on the immune system , especially in the area of the gut - associated lymphoid tissue (
GALT ) . -
pubmed
Assessment of an LC -
MS method for plasma quantification of the new immunosuppressant
FK778 through comparison with HPLC - UV . -
pubmed
The aim of this work was to develop an HPLC -
MS method to measure
FK778 in plasma for pharmacokinetic studies . -
pubmed
After precipitation of plasma
proteins with
acetonitrile , the supernatant was injected onto an analytical RP -
C18 column . -
pubmed
The aim of this study was to investigate whether
aglepristone , a
progesterone receptor antagonist , can be used to treat this form of
canine acromegaly . -
pubmed
Blood samples were collected before
MPA administration , immediately before , during , and 3.5 and 5.5 weeks after the last administration of
aglepristone for determination of the plasma concentrations of
GH and
IGF - I. -
pubmed
In addition , blood samples for the determination of the 6 - h plasma profile of
GH were collected before
MPA administration , before
aglepristone administration , and 1 week after the last
aglepristone treatment . -
pubmed
MPA administration resulted in a significant increase of the mean plasma
IGF - I concentration , whereas analysis of the pulsatile plasma profile demonstrated a trend ( P=0.06 ) for a higher mean basal plasma
GH concentration and a higher mean AUC(0 ) for
GH . -
pubmed
Treatment with
aglepristone resulted in a significant decrease of the mean plasma
GH and
IGF - I concentrations . -
pubmed
Analysis of the pulsatile plasma profile showed a trend ( P=0.06 ) for a lower mean basal
plasma GH concentration and a lower mean AUC(0 ) for
GH 1 week after the last
aglepristone treatment compared with these values before
aglepristone administration . -
pubmed
Three and a half and 5.5 weeks after the last
aglepristone administration the mean plasma
IGF - I concentration increased again . -
pubmed
administration of the
dopamine D1 receptor agonist (
SKF38393 ) ,
dopamine D2 receptor agonist (
quinpirole ) and
dopamine D2 receptor antagonist (
sulpiride ) not only reversed the effect of pre - training
morphine treatment , but also increased this action of the drug . -
pubmed
administration of
dopamine D1 receptor antagonist (
SCH23390 ) prevented the restoration of memory by
morphine . -
pubmed
In conclusion , the
morphine - induced recovery of memory , on the test day , seems to be induced , at least in part , through
dopamine receptors . -
pubmed
The frequencies of potential susceptibility biomarkers , the
UGT1A6 - Arg184Ser and the
SULT1A1 - Arg213His polymorphisms , were not associated with urinary
BPA levels , either as single genes or in combination . -
pubmed
Lower prevalence of the
OCT2 Ser270 allele in patients with
essential hypertension . -
pubmed
The
Organic Cation Transporter 2 ,
OCT2 (
SLC22A2 ) , has been implicated in renal
dopamine handling as well as in the inactivation of circulating
catecholamines and is supposed to be involved in blood pressure regulation . -
pubmed
SLC26A4 (
pendrin ,
PDS ) is a
Na+-independent ,
Cl-/
HCO3-/
OH- exchanger that is expressed in the apical regions of type B and non - A , non - B intercalated cells within the cortical collecting duct ( CCD ) , the connecting tubule and the distal convoluted tubule where it mediates
HCO3 - secretion and
Cl- absorption . -
pubmed
SLC26A4 is upregulated with
aldosterone analogues and with Cl- restriction . -
pubmed
In conclusion ,
SLC26A4 regulates blood pressure and arterial pH , likely by participating in the renal regulation of net acid and
Cl- excretion . -
pubmed
PHB overexpression increases the expression of
glutathione - S - transferase pi and protects from oxidant - induced depletion of
glutathione . -
pubmed
Finally ,
PHB overexpression decreases accumulation of reactive
oxygen metabolites , as well as increased permeability induced by oxidative stress in intestinal epithelial cells . -
pubmed
To examine the regulation of
androgen sensitivity in hair follicles , we focused on
androgen receptor co - activator
Hic-5 /
ARA55 . -
pubmed
Western blotting revealed rapid
tyrosine phosphorylation of
focal adhesion kinase (
FAK ) and
Src kinase in response to
TGF - beta1 ; inhibition of both of these
kinases using pp2 (
4 - amino-5 -( 4 - chlorophenyl )- 7 -( t - butyl ) pyrazolo [ 3,4 - d ] pyrimidine ) , ameliorates
TGF - beta1 - induced monolayer permeability . -
pubmed
Activation of
FAK /
Src requires activation of the
epidermal growth factor receptor downstream of the
TGF - beta receptors , and is blocked by the
epidermal growth factor receptor inhibitor
AG1478 . -
pubmed
Rearrangement of
paxillin and
vinculin by
TGF - beta1 is not blocked by the
FAK /
Src inhibitor , pp2 , or by
SB431542 inhibition of the
TGF - beta type I receptor ,
anaplastic lymphoma kinase 5 ; however ,
pp1 (
4 - Amino-5 -( 4 - methylphenyl )- 7 -( t - butyl ) pyrazolo [ 3,4 - d ] pyrimidine ) , which inhibits both
type I and type II TGF - beta receptors , does block
paxillin and
vinculin rearrangement . -
pubmed
Proliferation of
SGC cells was determined by
methylthiazolyl tetrazolium (
MTT ) assay . -
pubmed
EPR spectra of the excited quartet and doublet molecular states of
( tetraphenylporphinato ) zinc ( II ) covalently bounded to
3 -( N - nitronyl - notroxide ) pyridine stable radical are modeled in terms of the spin - Hamiltonian given by the sum of the contributions from the radical and triplet moieties , and the interaction between them . -
pubmed
Piracetam and its derivatives improved the outcome by 30.2 % ( 95 % CI =
16.1 - 44.4 ) . -
pubmed
Lack of involvement of
lipocortin 1 in
dexamethasone suppression of
IL-1 release . -
pubmed
Treatment of peripheral blood monocytes with 2 mug / ml
lipopolysaccharide potently increased
IL-1beta release ( p = 0.001 ) and
dexamethasone ( 10(-7 ) M ) significantly reduced both resting and stimulated
IL-1beta release ( p = 0.009 ) . -
pubmed
T - cadherin is unique among the
family of type I cadherins , because it lacks transmembrane and cytosolic domains , and attaches to the membrane via a
glycophosphoinositol anchor . -
pubmed
However ,
Tcad1 is monomeric in the absence and presence of
calcium . -
pubmed
Specific
amino acid replacements compared to classical
cadherins reduce the size of the binding pocket for residue 2 and alter the backbone conformation and flexibility around residues 5 and 15 as well as many electrostatic interactions . -
pubmed
In addition , we also found that
BR regulated the transcription of genes related to many physiological processes , such as photoreaction ,
ion transportation and some metabolic processes . -
pubmed
The
3 - hydroxy group was derivatised to a
primary amine and subsequently the
thiazolidinone nucleus was generated at the
amino functionality through
DCC mediated one -
pot three - component reaction in good yields . -
pubmed
P - glycoprotein ( P - gp ) does not affect
melatonin absorption but transported
rhodamine 123 , a well - known P - gp substrate . -
pubmed
At univariate analysis , both
BNP and
IL-6 plasma concentrations were predictors of
death and heart transplantation , but only
BNP was a predictor of
death ; however , only
creatinine plasma level was an independent predictor of prognosis . -
pubmed
Airway mechanosensors [
slowly adapting receptor (
SARs ) and rapidly adapting receptors ] were inhibited by
lidocaine , whereas chemosensors (
C - fiber receptors and high - threshold
A delta - receptors ) were stimulated . -
pubmed
The complete cDNA sequence of
Sirt2(T ) is 1059 bp , encoding a deduced protein of 352
amino acids which is 39
amino acids shorter at the N - terminus than
Sirt2 . -
pubmed
Dietary habits were evaluated using a food frequency questionnaire and the dietary antioxidant capacity was based on published values of Italian foods measured by three different assays :
ferric - reducing antioxidant power ( FRAP ) , total radical - trapping antioxidant parameters (
TRAP ) and
trolox - equivalent antioxidant capacity ( TEAC ) . -
pubmed
RESULTS : Positive associations were observed between dietary antioxidant capacity and
adiponectin concentration , as assessed with FRAP ( b+/-s.e.=0.012+/-0.005 , P=0.018 per 1 mmol
Fe ( II )/day ) ,
TRAP ( b+/-s.e.=0.030+/-0.013 , P=0.017 per 1 mmol
trolox equivalent / day ) and TEAC ( b+/-s.e.=0.025+/-0.012 , P=0.042 , per 1 mmol
trolox equivalent / day ) in multiadjusted analysis . -
pubmed
The sensitivity , specificity , accuracy , positive and negative predictive values of
FDG PET /
CT were respectively 97 % , 92 % , 95 % , 94 % and 96 % . -
pubmed
The dialysate - to - plasma ratio for
creatinine ( D / P
Cr ) was positively correlated to dialysate
CTGF concentration and estimated local peritoneal production of
CTGF . -
pubmed
The nodes of the
RD - RIRR network are comprised of models of individual mitochondria that include a mechanism of ROS - dependent oscillation based on the interplay between ROS production , transport , and scavenging ; and incorporating the
tricarboxylic acid (
TCA ) cycle , oxidative phosphorylation , and
Ca ( 2 + ) handling . -
pubmed
Local mitochondrial interaction is mediated by
superoxide (
O2.- ) diffusion and the
O2.(-)-dependent activation of an inner membrane anion channel (
IMAC ) . -
pubmed
Outer membrane proteins (
OMPs ) were analysed by
SDS - PAGE and
OmpA by matrix - assisted laser desorption ionization time - of - flight / time - of - flight mass spectrometry . -
pubmed
CONCLUSIONS : The ' cefpiromase ' or ' cefepimase ' ESBL phenotype of the clinical isolates non - susceptible to
cefpirome and
cefepime resulted from high expression of
TEM-1 or
OXA-1 beta - lactamases combined with loss of
porins . -
pubmed
Based on the comparison of deconvoluted mass spectra with spectra databases as well as experimentally derived retention indices with literature values , 38 metabolites were identified , which belong to the major classes of plant volatiles including
C6 - compounds ,
terpenes ( including modified
terpenes ) ,
aromatic compounds ,
alcohols and
n - alkanes . -
pubmed
Our preliminary data indicate that defence related pathways such as the
mevalonate and/or alternative
isopentenyl pyrophosphate- , the
lipoxygenase- (
LOX ) as well as the phenylpropanoid pathway are affected in root galls as a response to phylloxera attack . -
pubmed
Single 20- and 40 - mg doses of
vorapaxar completely inhibited
thrombin receptor activating peptide (
TRAP)-induced
platelet aggregation ( & gt;80 % inhibition ) at 1 h and sustained this level of inhibition for & gt;=72 h. Multiple doses yielded complete inhibition on Day 1 ( 5 mg / day ) and Day 7 ( 1 and 3 mg / day ) . -
pubmed
Both
BMD and histological examinations of femurs showed osteoporotic changes after ovariectomy , while systemic treatment with
ZOL restored mineralized bone . -
pubmed
Micro -
CT examination demonstrated that OVX group showed significant decrease of mineralized bone and implant - bone contact when compared with sham control , whereas both systemic and local treatments of
ZOL significantly increased mineralized bone and implant - bone contact in ovariectomized animals . -
pubmed
OBJECTIVE : To investigate the selective killing effect of
herpes simplex virus - thymidine kinase /
ganciclovir ( HSV - tk /
GCV ) suicide gene system controlled by
human IGF - II P4 promoter on
HCC cells in vitro . -
pubmed
We show that half of identified
E16 -
P0 mDA neurons spontaneously generate non - synaptic , intrinsically driven
Ca ( 2 + ) spikes and
Ca ( 2 + ) plateaus mediated by N- and L - type voltage - gated
Ca ( 2 + ) channels . -
pubmed
Starting from E18 -
P0 , half of the mDA neurons also reliably generate overshooting
Na (+ ) spikes with an abrupt maturation at birth (
P0 = E19 ) . -
pubmed
At that stage ( E18 -
P0 ) , dopaminergic terminals release
dopamine in a
calcium - dependent manner in the striatum in response to local stimulation . -
pubmed
Polymeric
copper ( II ) complex ,
[ Cu ( Hacm )( 2 )( na )( 2 )( H ( 2 ) O )( 2 ) ] [ H ( 2 ) acm ;
acetazolamide ,
na ;
nicotinamide ] was synthesized and characterized by spectroscopic ( IR ; infrared spectroscopy ,
EPR ; electron paramagnetic resonance ) , structural ( XRD ) and voltammetric structural ( CV ) methods . -
pubmed
The
copper ( II ) compound crystallizes in the triclinic space group
P1 , Z=1 , with the unit - cell dimensions : a=7.672 ( 5)A , b=8.681 ( 5)A , c=11.938 ( 5)A , alpha=90.807 ( 7 ) , beta=98.616 ( 5 ) and gamma=110.647 ( 5 ) . -
pubmed
The powder
EPR spectrum of
copper ( II ) complex have indicate that the paramagnetic center is in a tetragonal symmetry with the
Cu ( 2 + ) ion having a distorted octahedral geometry . -
pubmed
The magnetic properties of the porous metal - organic complex
Co ( bpy )( 1.5 )( NO ( 3 ))( 2 ) ( bpy = 4,4 '- bipyridine ) , investigated by SQUID magnetometry ,
EPR and heat capacity measurements , are reported . -
pubmed
Amperometric determination of total
phenolic content in wine by
laccase immobilized onto
silver nanoparticles /
zinc oxide nanoparticles modified gold electrode . -
pubmed
A method is described for construction of a highly sensitive amperometric biosensor for measurement of total
phenolic compounds in wine by immobilizing
laccase covalently onto nanocomposite of
silver nanoparticles ( AgNPs)/
zinc oxide nanoparticles ( ZnONPs ) electrochemically deposited onto gold (
Au ) electrode . -
pubmed
Wip1 knockdown or inhibition enhanced DNA damage signaling and resensitized
oral SCC cells to
cisplatin . -
pubmed
Finally , using xenograft
tumor models , we showed that
Wip1 upregulation promotes
tumorigenesis and its inhibition improves the
tumor response to
cisplatin . -
pubmed
Synthesis of novel
azo - resveratrol ,
azo - oxyresveratrol and their derivatives as potent
tyrosinase inhibitors . -
pubmed
Particularly , introduction of
hydroxyl or
methoxy group into the
4 - hydroxyphenyl moiety diminished or significantly reduced mushroom
tryosinase inhibition . -
pubmed
Among the synthesized
azo compounds ,
azo - resveratrol ( 5 ) showed the most potent mushroom
tyrosinase inhibition with an IC(50 ) value of IC(50)=36.28 + - 0.72 muM , comparable to that of
resveratrol , a well - known
tyrosinase inhibitor . -
pubmed
Comparisons between SGAs have shown that treatment with
olanzapine was associated with greater
weight gain and increased
cholesterol levels , and that treatment with
risperidone was associated with a greater increase in
prolactin levels . -
pubmed
Peptidomimetics based on the N - terminal stretch of the first 11
amino acids of the
PTH have been investigated as potential lead compounds for the treatment of
osteoporosis . -
pubmed
On the basis of a peptide reported in the literature , referred to here as the Parent Peptide (
H - Aib - Val - Aib - Glu - Ile - Gln - Leu - Nle - His - Gln - Har - NH2 ) , we conducted systematic
SAR analyses to investigate the effects of altering peptide hydrophobicity on
PTH receptor functional potency as measured by the
cAMP (
cyclic adenosine monophosphate ) accumulation and
beta - arrestin recruitment assays . -
pubmed
Among hydrophobic residues , we found that the
Val2 position shows the least flexibility in terms of the
SAR studies , whereas the
Leu7 position appeared to be most flexible . -
pubmed
The somatic cell count ( SCC ) , total bacterial count (
TBC ) in milk ,
cyclooxygenase (
COX ) activity , phagocytic activity , production of
hydrogen peroxide (
H2O2 ) and
myeloperoxidase (
MPO ) activity in milk leukocytes were evaluated after intramammary infusion of BRM in quarters inflicted with SCM . -
pubmed
Significant enhancement of phagocytic activity ,
H2O2 and
MPO activities indicate the priming of resident milk leukocytes in response to BRM infusion . -
pubmed
We explored the association of serum
uric acid ( UA ) concentrations with pro - inflammatory state and
high - density lipoprotein (
HDL ) dysfunction . -
pubmed
Linear regression model revealed fasting
triglycerides ( 1.86 mg / dl variance ) , male sex , and
gamma - glutamyl transferase and age as covariates of UA levels in women . -
pubmed
The major
fatty acids were C15 : 0 iso , summed feature 3 ( C16 : 1omega6c and/or C16 : 1omega7c / t ) , C16 : 1omega5c and
C15 : 0 anteiso . -
pubmed
The effect of age , sex , blood
sugar ,
GCS , CNS , and
seizure type on
stroke outcome and time to in - hospital
death in EPASS was tested on logistic regression and Cox proportional hazard regression . -
pubmed
Initial response rates were poor , however they have slowly but steadily improved , such that with the addition of the
nucleotide analogue
ribavirin and the pegylation of
interferon , over 50 % of infected individuals could be cured with a course of therapy . -
pubmed
Although the first DAAs were combined with
peginterferon and
ribavirin , with the rapid progress in the field , it is likely that
interferon - free therapy will be available for most patients in the relatively near future . -
pubmed
In the short term ,
peginterferon will be required with either the
protease inhibitor
simeprevir , or the
nucleotide analogue
polymerase inhibitor ,
sofosbuvir , for the treatment of
genotype 1 infection . -
pubmed
Coincidental
metabolic syndrome [ adjusted odds ratio ( aOR ) 2.0 , 95 % confidence interval ( CI ) 1.1 - 3.5 , P = 0.015 ] , central
obesity ( aOR 2.0 , 95 % CI 1.0 - 4.1 , P = 0.05 ) and low level of
high - density lipoprotein cholesterol ( aOR 1.9 , 95 % CI 1.0 - 3.7 , P = 0.04 ) were associated with
liver fibrosis progression independent of change in viral load and
ALT level . -
pubmed
TLR agonists induced
PGE2 in macrophages exclusively via
IL-1beta - independent mechanisms . -
pubmed
Blocking of
TLR4 endocytosis during LPS priming prevented the increase in
PGE2 production by exogenous
IL-1beta . -
pubmed
Our data showed that
TLR2 agonists induce
PGE2 in monocytes independently from
IL-1beta . -
pubmed
In contrast ,
anthocyanin inductive conditions (
AIC ) provided by high
sucrose media are characterized by high accumulation of
A9 * and A5 relative to other stress conditions . -
pubmed
,
insulin ,
glucose ,
cholesterol ) ,
insulin signaling components ( e.g. -
pubmed
CR decreased plasma levels of
insulin ,
glucose ,
cholesterol and
leptin , and increased hepatic
IR beta - subunit and
IR pY1158 levels as well as
IR ,
IRS-1 and
GLUT-2 gene expression compared to ad libitum feeding , showing a significant beneficial diet intervention effect . -
pubmed
On the contrary ,
GH increased levels of
glucose ,
cholesterol and
leptin in plasma , and p - mTOR or p -
p38 in livers , and decreased plasma
adiponectin and hepatic
IR beta - subunit compared to saline treatment . -
pubmed
Centrifugal partition chromatography ( CPC ) was used as a fractionation and purification tool , guided by an in vitro
adenosine diphosphate (
ADP ) ,
arachidonic acid or
collagen - platelet aggregation assay . -
pubmed
Unlike
2G12 , which is strictly specific for the high - density
Man9GlcNAc2Asn ( Man9 )-clusters ,
GNA recognizes a number of N - glycan cryptic
sugar moieties . -
pubmed
Recently , the
5 - Lipoxygenase (
5 - LO , a key enzyme metabolizing
arachidonic acid to produce
leukotrienes ) inhibitors have been showed to protect brain against
ischemic damage in animal model of
cerebral ischemia . -
pubmed
Superoxide dismutase (
SOD , the antioxidant enzyme ) activities and
malondialdehyde (
MDA , an oxidative stress biomarker ) contents were detected . -
pubmed
NF - kappaBp65 expression and
MDA content decreased significantly , and
SOD activities increased significantly in hippocampus . -
pubmed
Complete work up including physical examination , laboratory and imaging studies ( complete blood count ,
b - HCG , hormonal levels and ultrasonography ) is needed , and appropriate treatment with combined
oral contraceptives is administered accordingly . -
pubmed
After surgery , the
serum ACTH level was almost normalized , and the
dexamethasone ( 1 mg ) suppression test showed significant suppression of
ACTH . -
pubmed
EXPERT OPINION : Preclinical and retrospective data for
erlotinib provide evidence of CNS penetration , and objective responses in the setting of BM from
EGFR mutated
NSCLC . -
pubmed
Phase II / III data however , from non -
EGFR mutation enriched populations , have demonstrated no benefit in progression free or overall survival with the addition of
erlotinib to metastasis directed radiotherapy . -
pubmed
Moreover , we suggest some unexplored pathways such as
sphingolipid metabolism ,
insulin - vesicle trafficking , regulation of transcription / transduction by miRNA-30 , trafficking proteins , and cell cycle proteins that could play important roles in the process mentioned above for further investigation . -
pubmed
We further show that
iron depletion potentiates the antistaphylococcal activity of
HHQ , as well as
2 - heptyl-4 - hydroxyquinoline - N - oxide (
HQNO ) , another AQ that functions as a
cytochrome B inhibitor . -
pubmed
Linkage disequilibrium between the
CYP2C19 * 2 ,* 17 and
CYP2C9 * 1 alleles and impact of
VKORC1 ,
CYP2C9 ,
CYP2C19 gene polymorphisms and gene - gene interactions on
warfarin therapy . -
pubmed
This study aims to investigate the allele distribution of
VKORC1 ,
CYP2C9 and
CYP2C19 , contribution of different allele variants and possible gene - gene interaction on
warfarin therapy . -
pubmed
Patient with
VKORC1 - 1639 G & gt ; A ,
CYP2C9 * 2 and
CYP2C9 * 3 genetic variants need significant lower
warfarin dose than patient with wild type allele of
VKORC1 1639 G or
CYP2C9 * 1 . -
pubmed
There is no significant differences between
CYP2C19 allele variants for
warfarin stable dose and INR & gt ; 5 event . -
pubmed
Because of the complete linkage disequilibrium between
CYP2C19 * 2 ,* 17 and
CYP2C9 * 1 , patient with
CYP2C19 * 2 /* 2 , * 2 /* 17 and * 17 /* 17 genotypes tend to have higher
warfarin dose than patient with
CYP2C19 * 1 /* 1 genotype . -
pubmed
Stepwise regression analysis showed that
VKORC1 ,
CYP2C9 ,
body mass index (
BMI ) , age and gender were included as a factor significantly contributing to
warfarin dose , whereas
CYP2C19 did not contribute to
warfarin dose . -
pubmed
No statistically significant interaction between
CYP2C9 and
VKORC1 on
warfarin dose and INR & gt ; 5 event was detected in univariate general linear model analysis . -
pubmed
Our study suggests that polymorphic variants of
VKORC1 and
CYP2C9 affect
warfarin dose independently , whereas
CYP2C19 did not contribute to
warfarin therapy . -
pubmed
We found that specific NIR spectral absorbances correlated with
MIR measurements of chemical composition , including relative amount of
PGA ( R = 0.86 , p = 0.02 ) ,
collagen ( R = 0.88 , p = 0.03 ) , and proteoglycan ( R = 0.83 , p = 0.01 ) . -
pubmed
Then , fasting blood
glucose , blood
insulin level and
insulin resistance index were measured with enzymatic methods . -
pubmed
Immunofluorescent labeling was carried out to visualize the
insulin receptor substrate 2 (
IRS2 ) ,
Obese receptor (
Ob - R , a
leptin receptor ) ,
voltage - dependent anion channel protein 1 (
VDAC1 ) ,
cytochrome C ( cytC ) ,
5 - methylcytosine ( 5 - mC ) positive cells in hippocampal
CA1 area . -
pubmed
The present work provides a detailed study on the structural variants of the
tetrasaccharide fragments of this binding site afforded following treatment of a heparin with
heparin lyase II . -
pubmed
Appropriate concentrations of
betaine and
L - arginine for subtype - selective inhibition in
SNAT - overexpressing cells were identified . -
pubmed
This morphological phenotype was rescued by co - expression of
TCF4 plus
calmodulin in a
calcium - dependent manner and by dampening neuronal excitability through co - expression of an
inwardly rectifying potassium channel (
Kir2.1 ) . -
pubmed
For we believe the first time , we show that
N - methyl - d - aspartate ( NMDA ) receptor - dependent
Ca2 + transients are instructive to minicolumn organization because
Crispr /
Cas9 - mediated mutation of
NMDA receptors rescued
TCF4 - dependent morphological phenotypes . -
pubmed
BACKGROUND /
AIM : To evaluate the efficacy of conversion surgery following S-1 plus
cisplatin (
CS ) or
oxaliplatin (
SOX ) chemotherapy . -
pubmed
The recombinant protein had 24 kDa apparent molecular mass , suggesting being expressed as a glycoprotein , and was purified from serum - free conditioned medium by the combination of
Diethylaminoethanol Sepharose ion exchange and Sephacryl S-200
HR gel filtration . -
pubmed
Moreover ,
hypothyroidism significantly decreased the content of
ACh ( 29.8 % ) and activity of
AChE ( 27.8 % ) , which were restored to control values by T4 admi - nistration . -
pubmed
At the protein level ,
hypothyroidism increased the levels of
syt-1 and
SNAP-25 in the PFC , both of which were not restored to control values following T4 administration , while concurrent administration of T4 and
DON was able to induce this effect . -
pubmed
Dopamine cross - reacts with
adrenoreceptors in cortical astrocytes to induce
BDNF expression ,
CREB signaling and morphological transformation . -
pubmed
However , it has remained unknown which receptors mediate
dopamine - induced
BDNF upregulation in astrocytes . -
pubmed
We show that in cortical astrocytes
BDNF exon
IV and exon VI containing mRNAs are induced by
dopamine and
norepinephrine via
CREB - dependent signaling and that this regulation is mediated by a mechanism that is distinct from activity - dependent
CREB - mediated activation of
BDNF transcription in neurons . -
pubmed
We also show that regulation of
BDNF promoters
IV and VI by
catecholamines requires a distal regulatory element in the
BDNF locus . -
pubmed
Finally , we demonstrate that
dopamine - induced astrocyte stellation and induction of
CREB signaling are mediated by cross - reaction of
dopamine with
beta adrenoreceptors . -
pubmed
FpvAI is a
TonB - dependent transporter (
TBDT ) that actively imports the small siderophore
ferripyoverdine (
Fe - Pvd ) by coupling to the proton motive force (
PMF ) via the inner membrane ( IM ) protein
TonB1 . -
pubmed
The crystal structure of the N - terminal domain of
pyoS2 (
pyoS2NTD ) bound to
FpvAI ( Kd = 240 pM ) reveals that the
pyocin mimics
Fe - Pvd , inducing the same conformational changes in the receptor . -
pubmed
GGT ,
AST and
ALT levels were tested during weeks 14 - 18 of gestation and oral
glucose tolerance test was conducted during 24 - 28 weeks to screen
GDM . -
pubmed
Here , we discuss the biosynthetic routes toward the
N - C macrocyclization of linear peptide precursors , specifically , those transformations that are catalyzed by
peptidases . -
pubmed
AIM : To evaluate renal safety of
TDF according to the
Kidney Disease : Improving Global Outcomes ( KDIGO ) guidelines . -
pubmed
Participation of
fatty acids on the
AR through
PKA and
PKC pathways was evaluated using a specific inhibitor of these enzymes . -
pubmed
When spermatozoa were incubated with
PKA or
PKC inhibitors , there was a significant reduction of
arachidonic acid - induced acrosome reaction (
AR ) ( P & lt ; 0.05 ) , and inhibition by
PKA inhibitor is stronger than that by
PKC inhibitor . -
pubmed
Conclusions : Incorporation of
unsaturated fatty acids , especially
oleic acid , into
triglycerides and
phospholipids provides prerequisite energy for
AR . -
pubmed
Cholesterol efflux by
arachidonic acid triggers
AR . -
pubmed
Arachidonic acid activated
PKA and
PKC pathway participate in induction of the
AR . -
pubmed
Primary metabolic transformation involved aromatization of the
dihydronaphthyridine moiety of metabolite
M1 as a major clearance pathway with a second oxidative pathway leading to M4 . -
pubmed
Plasma
FGF21 concentration consistently increased by threefold only after the two low - protein ( 3 % ) overfeeding diets , one high in
carbohydrate ( 75 % ) and the other high in fat ( 46 % ) , with larger increases in
FGF21 being associated with greater increases in 24 - h EE . -
pubmed
We had previously observed that after severe
burn trauma , increased energy expenditure is temporally related to a marked reduction in serum concentrations of both
parathyroid hormone and
fibroblast growth factor 23 , both of which have phosphaturic effects . -
pubmed
Here we used molecular modelling to produce a range of ligand binding domain ( LBD ) point mutants of
GluK1 - 3 KAR subunits with and without altered agonist efficacy to further investigate the role of
glutamate binding in surface trafficking and activation of homomeric and heteromeric
KARs using
endoglycosidase digestion , cell surface biotinylation and imaging of changes in intracellular
Ca2 + concentration [
Ca2 +]i . -
pubmed
However ,
[ Ca2 +] i imaging indicates that the occupancy of both
GluK2 and
GluK5 LBDs is required for the full activation of
GluK2 /
GluK5 heteromeric
KAR channels . -
pubmed
RESULTS : Compared to model group ,
OPCRR treatments significantly decreased the serum lipid profiles including total
cholesterol , total
triglycerides ,
low - density lipoprotein cholesterol (
LDL - C ) and
ox - LDL and increased the
high - density lipoprotein cholesterol (
HDL - C ) ; significant increased serum antioxidant enzymes (
SOD and
GSH - Px ) and decrease of
MDA content as a product of lipid peroxidation ; lowered serum levels of
TNF - alpha ,
IL-1beta ,
IL-6 ,
ICAM-1 and
VCAM-1 and enhanced
IL-10 level ; increased the serum release of
nitric oxide and expression of
iNOS in aortic , whereas decreased the expression of
eNOS . -
pubmed
CONCLUSION :
OPCRR can improve the progress of
atherosclerosis by regulation of lipid metabolism , restoring of the antioxidant capacities , and attenuation of pro - inflammatory
cytokines and chemcytokines release , and improving the
endothelial dysfunction indicated by
nitric oxide system . -
pubmed
The
mitochondria - associated glycerol-3 - phosphate acyltransferase 1 (
GPAT1 ) catalyzes the first committed step in
glycerolipid synthesis , and its activity diverts
fatty acids from mitochondrial beta - oxidation . -
pubmed
Them2 , a mitochondria - associated
acyl - CoA thioesterase , also participates in the trafficking of
fatty acids into oxidative versus
glycerolipid biosynthetic pathways . -
pubmed
Preserved
insulin receptor activity was supported by
Thr-308 phosphorylation of
Akt following
GPAT1 overexpression in
Them2 -/- hepatocytes . -
pubmed
These findings suggest a pathogenic role of
Them2 in the biosynthesis of
glycerolipid metabolites that promote hepatic
insulin resistance . -
pubmed
The knockdown of intracellular
PRDX6 significantly enhances
LOOH and ferroptotic cell
death triggered by ferroptosis inducers (
Erastin and
RSL-3 ) , which is correlated with the transcriptional activation of
heme oxygenase-1 . -
pubmed
Moreover , overexpression of
heme oxygenase-1 enhances both
Erastin- and
RSL-3 - triggered LOOH , suggesting that
heme oxygenase-1 mediates
PRDX6 silencing - enhanced ferroptosis . -
pubmed
More importantly , the application of a specific
PRDX6 phospholipase A2 (
iPLA2 ) inhibitor ,
MJ-33 , synergistically enhances the ferroptosis induced by
Erastin , suggesting that
PRDX6 removes
LOOH through its
iPLA2 activity . -
pubmed
Achieving
CR was associated with older age ( p = 0.033 ) , no
arthralgia /
arthritis at baseline ( p = 0.001 ) , and no prior use of
cyclophosphamide ( p = 0.003 ) or
hydroxychloroquine ( p = 0.016 ) . -
pubmed
Overall , compared with the control group for
heart failure ,
liraglutide treatment significantly can reduce
NT - proBNP ( Std . -
pubmed
CONCLUSIONS :
Liraglutide treatment has an important ability to reduce
NT - proBNP and improve 6MWT for
heart failure , but shows no important influence on LVEF , LVEDV , LVESV , hospitalization for
heart failure , major adverse cardiovascular events , and
cardiac death . -
pubmed
After
SCI , chondroitin
sulfate proteoglycans ( CSPGs ) inhibit axon regrowth via putative interactions with the
LAR - family protein tyrosine phosphatases ,
PTPsigma and
LAR , localized on the injured axon tips . -
pubmed
The saline soil was amended with BC at rates of 0 , 7.5 t ha-1 ( BC1 ) , 15 t ha-1 (
BC2 ) and 30 t ha-1 ( BC3 ) alone or combined with
fulvic acid ( 1.5 t ha-1 ) compared with control . -
pubmed
Although
Na + ,
Cl- and
SO42 - in BC and /or
FA treatments significantly decreased , the pH value increased up to 9.0 as the
CO32- +
HCO3 - content increased . -
pubmed
Total organic
carbon ( TOC ) and
phosphorus (
TP ) responded positively to biochar addition rate . -
pubmed
Synthesis , Antimicrobial Activity and Molecular Docking of Novel
Thiourea Derivatives Tagged with
Thiadiazole ,
Imidazole and
Triazine Moieties as Potential
DNA Gyrase and
Topoisomerase IV Inhibitors . -
pubmed
coli
Topoisomerase IV ( IC50 = 0.33 + - 1.25 and 19.72 + - 1.00 microM , respectively ) in comparison with
novobiocin ( IC50 values 0.28 + - 1.45 and 10.65 + - 1.02 microM , respectively ) . -
pubmed
Moreover , pre - treatment with
PLGA /
SBN nanoparticles reduced elevated
transaminase and
bilirubin levels in blood ,
caspase 3 activation , and morphological histology changes in liver tissue upon
DTIC treatment . -
pubmed
Gaucher disease , the most prevalent
metabolic storage disorder , is caused by mutations in the
glucocerebrosidase gene
GBA1 , which lead to the accumulation of
glucosylceramide (
GlcCer ) in affected cells . -
pubmed
With this synthetic mRNA - based rapid differentiation method , we found that the metabolic defect in
GD1 patient cells can be rescued by the overexpression of wild - type
GBA1 or the treatment with an inhibitor for
GlcCer synthesis . -
pubmed
We also evaluated for associations between
LAKR and the metabolic state of
cancer cell lines , given that
KRAS has been shown to regulate
fatty acid synthesis . -
pubmed
FASN inhibitors such as
TVB-3664 showed synergistic effects with the
KRASG12C inhibitor
MRTX849 in LUAD cells with
KRASG12C and
LAKR , including an in vivo trial using a xenograft model . -
pubmed
Type 3 cells are stainable with PAS ,
ATh , AB , AF , and
aniline blue and are distributed in an area comprises of anteroventral RPD and anterodorsal
PPD . -
pubmed
We measured the binding of
[ 14C ] iodoacetamide labeled
heavy meromyosin subfragment-1 (
S-1 ) to
F - actin by sedimenting the
actin - S-1 complex and assaying the radioactivity remaining in the supernatant . -
pubmed
Kd was not altered when the
troponin -
tropomyosin complex was bound on the
actin , nor was it affected by free
calcium concentration in the range 10(-4 ) to 10(-9 ) M. Measurements of the displacement of labeled S-1 from
actin by
native S-1 showed labeling had not altered Kd . -
pubmed
The fragments
Boc - Lys ( Z )- Ile - Cys ( SiPr )- Gly - Lys(Z)(I ) and
Boc - Pro - His ( Trt )- Ile - Cys ( Trt )- Arg(Tos ) (
II ) were synthesized conventionally . -
pubmed
After the hydrolysis of fragment
II with
6N HCl ,
allo - isoleucine could be detected by gas chromatography and
amino - acid analysis . -
pubmed
coli BB core was studied by beta - elimination and using the information obtained with
KDO -
PN . -
pubmed
The basal [
Ca2 +]i of cells at
rest was not altered by long - term treatment with
NGF , neither in the cell bodies nor in the growth cones . -
pubmed
The
dihydropyridine (
DHP )
nifedipine alone , or together with omega - conotoxin (
omega - CgTX ) , were similarly effective in inhibiting the
K (+)-induced increase in [
Ca2+]i in untreated and
NGF - treated cell bodies , arguing for a preferential distribution of L - type
Ca2 + channels in this cell area . -
pubmed
By contrast , after 6 - 7 days exposure to
NGF the
K (+)-induced initial transient rise of [
Ca2+]i in growth cones was very sensitive to omega - CgTX , whereas
nifedipine affected only the sustained rise . -
pubmed
During
NGF - induced differentiation there is a predominance of N - type
Ca2 + channels in growth cones , while
Ca2 + stores are of minor importance in these structures . -
pubmed
The half - lives of
R - and S - flecainide in PMs ( R 19.3 h ; S
16.1 h ) were approximately twice those observed in EMs ( R 8.8 h ; S 9.1 h ) . -
pubmed
Specific binding sites for [ 3H]Ro5 - 4864 and
[ 3H ] PK 11195 were also assayed and found to cofractionate with each other and in a manner which coincided with the photolabeled
PKBS profile . -
pubmed
Scatchard analysis of both subpopulations revealed a single class of binding sites for
[ 3H ] Ro5 - 4864 with an apparent KD of 14 nM for Q - I and 22 nM for Q -
II ; these affinities were slightly lower than those found in mitochondrial membrane preparations used as the starting material for solubilization . -
pubmed
The rank order of potency to inhibit
[ 3H ] Ro5 - 4864 binding in both subpopulations was
PK 11195 greater than
Ro5 - 4864 greater than
diazepam greater than
clonazepam , in connection with the pharmacological specificity of
membrane - associated peripheral - type benzodiazepine binding sites . -
pubmed
These studies provide direct biochemical evidence that the recognition sites for
benzodiazepines and
isoquinoline carboxamides cofractionate in unison with the 17 - kD
PKBS protein , demonstrating an intimate relationship between this protein and the binding domains for peripheral - type
benzodiazepine ligands . -
pubmed
Eight of the first nine mutant
RTAs studied showed single -
amino - acid changes involving residues located in the proposed active - site cleft . -
pubmed
The respective rate constants , k1 and k2 , were determined using two
superoxide - generating systems (
xanthine oxidase /
xanthine as well as ionizing radiation ) . -
pubmed
OXANOH oxidation and
OXANO reduction are both inhibitable by
superoxide dismutase , pH - dependent ( 4.5 - 9.3 ) , and result in a steady state distribution of [
OXANO ] and
[ OXANOH ] , independent of their initial concentrations , i.e. -
pubmed
the
OXANO /
OXANOH couple exhibits a metal - independent
superoxide dismutase - like function . -
pubmed
Native , heparinized , citrated and
EDTA blood and platelet - rich plasma (
PRP ) were forced through the filter . -
pubmed
ADP- and
thrombin - induced aggregation requires
GPrIIb / IIIaand fibrinogen . -
pubmed
The
amino acid sequences of
APRTs have been highly conserved throughout evolution . -
pubmed
Retention of an elevated
CpG dinucleotide content , despite loss of sequence homology , suggests that there may be selection for
CpG dinucleotides in these regions and that their maintenance may be important for
APRT gene function . -
pubmed
Sodium warfarin was routinely used in an amount to reduce the
prothrombin activity in a range of 20 - 30 % normal by thrombotest . -
pubmed
Heparin therapy was instituted for the prevention of
thromboembolism , when
prothrombin activity recovered to 50 % normal by thrombotest , in 5 of 7 cases . -
pubmed
Heparin therapy was maintained by continuous intravenous infusion to produce
ACT of 130 to 150 seconds . -
pubmed
Heparin therapy was stopped when
prothrombin activity reached a therapeutic level with
sodium warfarin . -
pubmed
The
dopamine hypothesis of
schizophrenia received strong support when a two- to three - fold elevation of
D2 receptor densities was demonstrated by positron emission tomography ( PET ) and
[ 11C ] N - methylspiperone (
[ 11C ] NMSP ) . -
pubmed
Preincubation of NK cells with three
anti - CD7 mAb (
Leu-9 , 8H8 - 1 , and
LAU - A1 ) failed to block the binding of
IgM to the
Fc mu R. -
pubmed
In current -
clamp mode , superfusion with
L - leucine caused a hyperpolarization while superfusion with
L - arginine depolarized the oocyte . -
pubmed
In conclusion ,
rBAT - mediated transport of neutral and
dibasic amino acids is associated with net outward or inward currents , respectively , which may be caused by an exchange of neutral with
dibasic amino acids . -
pubmed
Forty seven units ordered a specific inspired
oxygen , the
rest ordering " air " as balance . -
pubmed
These observations support the suggestion that an unbalanced synthesis of
collagenase and
TIMP reported to occur following UV - A irradiation or during inflammatory conditions may be mediated by singlet
oxygen . -
pubmed
By using
Pax-5 and
Pax-8 recombinant proteins , we demonstrate that the binding activity of the
Prd domain is regulated through the oxidation / reduction of conserved
cysteine residues . -
pubmed
Mass spectrometry analysis and mutagenesis experiments demonstrate that the redox regulation is accomplished through the reversible formation of an intramolecular
disulfide bridge involving the
cysteines present in the
PAI subdomain , whereas the
RED subdomain appears quite insensitive to redox potential . -
pubmed
Here , we demonstrate that sequences essential for binding to the
CAS nuclear export factor are located near the
Imp alpha COOH terminus and include a critical
acidic motif . -
pubmed
Although point mutations introduced into this
acidic motif inactivated both
CAS binding and
Imp alpha nuclear export , a putative
leucine - rich nuclear export signal proved to be neither necessary nor sufficient for
Imp alpha nuclear export . -
pubmed
However , these two NLS - binding sites showed only minimal overlap , thus suggesting that the degeneracy of the Arm repeat region of
Imp alpha may serve to facilitate binding to similar but nonidentical
basic NLSs . -
pubmed
Fourier transform infrared analysis of purified
lactose permease : a monodisperse
lactose permease preparation is stably folded , alpha - helical , and highly accessible to
deuterium exchange . -
pubmed
The purification of monodisperse
lactose permease has been aided by the development of a
lacY gene product containing an
amino - terminal six
histidine affinity tag . -
pubmed
In the novel purification method described here ,
lactose permease is purified from
beta - dodecyl maltoside - solubilized membrane vesicles using three sequential column steps :
hydroxyapatite ,
nickel -
nitriloacetic acid (
Ni - NTA ) affinity , and cation - exchange chromatography . -
pubmed
Cytochrome P450 - dependent metabolism of
arachidonic acid . -
pubmed
The
cytochrome P450 - dependent metabolism of
arachidonic acid , the mechanisms of regulation of stereo- and regiospecificity by
cytochrome P450 isoenzymes , and the biological relevance of metabolites of the
arachidonic acid cascade is discussed in this review . -
pubmed
The comparison between
BuXI and
BvTI reactive site structure indicates differences at
Met59 ,
Thr66 and
Met67 residues . -
pubmed
The hydrolysis rate of quenched fluorescence peptide substrates based on BuXI reactive site sequence , Abz - VMIAALPRTMFIQ - EDDnp ( leading peptide ) , by
HuPK and
porcine pancreatic kallikrein (
PoPK ) is low , but hydrolysis is enhanced with
Abz - VMIAALPRTMQ - EDDnp , derived from the leading peptide shortened by removing the dipeptide
Phe - Ileu from the C - terminal portion , for
HuPK ( Km = 0.68 microM , k(cat)/Km = 1.3 x 10(6 ) M(-1 ) s(-1 ) ) , and the shorter substrate
Abz - LPRTMQ - EDDnp is better for
PoPK ( Km = 0.66 microM , k(cat)/Km = 2.2 x 10(3 ) M(-1 ) s(-1 ) ) . -
pubmed
The contribution of substrate
methionine residues to
HuPK and
PoPK hydrolysis differs from that observed with
factor Xa . -
pubmed
Fibroblast growth factor-8 (
FGF-8 ) is an important signaling
molecule in the generation and patterning of the midbrain , tooth , and limb . -
pubmed
First , it converts presumptive retinal
pigment epithelium (
RPE ) into NR . -
pubmed
However ,
GABA further elevated
CCK ( 10 pM)- ,
gastrin - releasing peptide ( 100 pM)- , or electrical field stimulation - induced pancreatic secretions of fluid and
amylase dose dependently . -
pubmed
The
GABA ( 30 microM)-enhanced
CCK - induced pancreatic secretions were completely blocked by
bicuculline ( 10 microM ) , a
GABA ( A ) receptor antagonist , but were not affected by
saclofen ( 10 microM ) , a
GABA ( B ) receptor antagonist . -
pubmed
The enhancing effects of
GABA ( 30 microM ) on
CCK - induced pancreatic secretions were not changed by
tetrodotoxin ( 1 microM ) but were partially reduced by
cyclo -( 7 - aminoheptanonyl - Phe - D - Trp - Lys - Thr[
BZL ] ) ( 10 nM ) , a
somatostatin antagonist . -
pubmed
Nonsteroidal anti - inflammatory drugs ( NSAIDs ) such as
aspirin ,
indomethacin (
IND ) ,
ibuprofen and newer
cyclooxygenase-2 selective NSAIDs ( e.g. -
pubmed
IND pre - treatment significantly inhibited
VEGF - induced Egr-1 mRNA expression by 29+/-4 % ( P<0.01 ) and protein expression by 41+/-8 % ( P<0.05 ) . -
pubmed
NS-398 inhibited
VEGF - induced Egr-1 mRNA and protein expression by 23+/-3 % and 35+/-4 % , respectively ( both P<0.01 ) . -
pubmed
Muscular
creatine phosphate values were not significantly changed in the TPN group , but the values increased in the TPN +
FDP group from 4.06 mumol / g.wt at the beginning to 4.93 mumol / g.wt at 24 h and 5.60 mumol / g.wt at 48 h ( P & lt ; 0.05 ) , with a
cytochrome oxidase increase in immunohistochemistry stain . -
pubmed
ProMMP-26 has the unique
Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp cysteine - switch motif that discriminates this
protease from all other
matrix metalloproteinases (
MMPs ) known so far . -
pubmed
MMPs become functionally active when proteolytic cleavage releases the prodomain and the PRCXXPD sequence and exposes the
zinc atom . -
pubmed
Here , we report that the
Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp motif is not functional in
proMMP-26 and consequently is not involved in the activation mechanisms . -
pubmed
The autolytic
Lys - Lys - Gln(59 ) downward arrow
Gln ( 60 )- Phe - His cleavage upstream of the
Pro - His ( 81 )- Cys - Gly - Xaa - Xaa - Asp motif induced the proteolytic activity of recombinant
proMMP-26 whereas any further cleavage inactivated the enzyme . -
pubmed
The
AR is a hormone - dependent transcription factor that activates expression of numerous
androgen - responsive genes . -
pubmed
Earlier labeling studies suggested that
nitrogen might be incorporated from a pathway involved in a
molybdenum - dependent nitrate reductase . -
pubmed
The
amino acid sequence deduced from the
moeA gene showed significant similarity to members of the
MoeA protein family and contains all the structural features that are highly conserved in the putative functional domains of
MoeA proteins . -
pubmed
mediterranei growth in a minimal medium with
nitrate as sole
nitrogen source , possibly because
nitrate reductase activity is diminished due to disruption of the
moeA gene . -
pubmed
Compared to the wild type
strain ,
moeA - disrupted mutants lost 95 % of their
rifamycin SV production capacity in complex fermentation media . -
pubmed
mediterranei , and that the
nitrogen assimilation pathway involved in
nitrate reductase is the major pathway for the genesis of the
amide nitrogen atom in the
rifamycin SV molecule . -
pubmed
BACKGROUND : Impairment of
insulin - stimulated
glucose transport is a characteristic of
type 2 diabetes . -
pubmed
[ Transformation in vivo of
dithiopropylthiamine in
cocarboxylase ] . -
pubmed
BACKGROUND : The differential tolerability profile of various
interferon ( IFN )- alpha preparations used in combination with
ribavirin for the treatment of
chronic hepatitis C needs to be elucidated . -
pubmed
OBJECTIVE : To test the hypothesis that
fibrinogen /
LDL - apheresis is as effective or superior to conventional therapy with plasma expanders and
prednisolone in the treatment of
SSHL . -
pubmed
INTERVENTIONS : Single
fibrinogen/
LDL - apheresis infusion of
prednisolone ( 250 mg , tapered by 25 mg daily ) , hydroxyethyl starch ( 500 ml , 6 % ) and
pentoxifylin ( 400 mg / day ) . -
pubmed
CONCLUSIONS :
Fibrinogen / LDLapheresis is at least equally effective compared to
prednisolone treatment in
sudden hearing loss . -
pubmed
Direct electrochemistry and electrocatalysis of
hemoglobin immobilized on
carbon paste electrode by
silica sol - gel film . -
pubmed
Direct electrochemical and electrocatalytic behaviors of
hemoglobin (
Hb ) immobilized on
carbon paste electrode (
CPE ) by a
silica sol - gel film derived from
tetraethylorthosilicate (
TEOS ) were investigated for the first time . -
pubmed
The formal potential of Hb heme
Fe ( III )/ Fe ( II ) couple varied linearly with the increase of pH in the range of 5.0 - 10.0 with a slope of 49.44 mV pH(-1 ) , which suggests that a proton transfer is accompanied with each electron transfer (
ET ) in the electrochemical reaction . -
pubmed
The immobilized Hb displayed the features of
peroxidase and gave excellent electrocatalytic performance to the reduction of
O2 ,
NO2 (- ) and
H2O2 . -
pubmed
The calculated apparent Michaelis - Menten constant was 8.98 x 10(-4)M , which indicated that there was a large catalytic activity of Hb immobilized on
CPE by sol - gel film toward
H2O2 . -
pubmed
On such a ground , the relevance of the starting tip - substrate distance in determining a non - invasive imaging has been investigated for a
plastocyanin mutant chemisorbed on
Au ( 111 ) electrodes . -
pubmed
In the present study , using two - dimensional
polyacrylamide gel electrophoresis and quadrupole time - of - flight mass spectrometry , we identified an
aspartate ammonia - lyase (
AspA ) which is upregulated upon induction with bronchoalveolar lavage fluid ( BALF ) . -
pubmed
In a subset of
K (+ ) channels , gating is regulated through the direct binding of ligands by large cytoplasmic
RCK domains . -
pubmed
Standard detergent solubilization of recombinantly expressed
MjK2 required the addition of a high
NaCl concentration to the extraction buffer for
MjK2 solubilization . -
pubmed
Purified
MjK2 reconstituted in liposomes generated only limited
Rb (+ ) uptake , blocked by
BaCl ( 2 ) . -
pubmed
As deduced from
amino acid sequence , as well as determined by gel - filtration and
SDS - polyacrylamide gel electrophoresis ,
ppBH4 is an active monomer of 46.5 kDa . -
pubmed
The difference in fluorescence of the free and
enzyme - bound
flavin strongly indicates that the increase in
enzyme activity in a pH - dependent manner is related to a protein - cofactor interaction . -
pubmed
Only one
amino acid residue ,
His75 , has an intrinsic pKa approximately 6.0 and is localized in proximity ( & lt;10 A ) to N5 -
N10 of the
isoalloxazine ring and , therefore , is able to participate in such an interaction . -
pubmed
There is a strong coupling between the fluorescence of the
enzyme - bound
flavin and the intrinsic
tryptophans , as demonstrated by energy transfer between
Trp47 and the
isoalloxazine ring of
flavin adenine dinucleotide (
FAD ) . -
pubmed
The pH - induced changes in intrinsic
tryptophan and
FAD fluorescence indicate that inhibition of the
FAD - binding enzyme at low pH is related to dissociation of the
flavin cofactor , due to protonation of its
adenine moiety . -
pubmed
In this work , seven fluorescent , internally quenched , decapeptides have been synthesized using the prototypical
cathepsin B selective substrate
Z - Phe - Arg - AMC as a lead , and used to identify the structural factors determining the susceptibility of peptides to hydrolysis at acidic and neutral pH values . -
pubmed
Peptides containing
Ala or
Phe at position 6 are good substrates for the
enzyme at both pH 5.0 and 7.4 . -
pubmed
By contrast , those containing
Glu ,
Asp ,
Lys or
Val are not cleaved at all by
cathepsin B at pH 7.4 , and are poorly hydrolyzed at pH 5.0 . -
pubmed
C8 is an oligomeric protein composed of a
disulfide - linked C8alpha - gamma heterodimer and a noncovalently associated
C8beta chain . -
pubmed
betaMACPF was also capable of binding independently to erythrocytes carrying
C5b-7 . -
pubmed
The native
enzyme has a molecular weight of 130,000 as measured by gel permeation chromatography , and contains four subunits ( mol wt 31,500 ) as measured by
sodium dodecyl sulfate gel electrophoresis . -
pubmed
No
selenium could be detected in the purified
enzyme . -
pubmed
The
enzyme is active with
H ( 2 ) O ( 2 ) and a variety of organic
hydroperoxides , including
13 - hydroperoxylinoleic acid , and is specific for
GSH as the
thiol substrate . -
pubmed
The antiapoptotic effect of
bortezomib was likely mediated by an increase in hepatic cellular
FLICE inhibitory protein (
c - FLIP ) levels , a potent antiapoptotic protein . -
pubmed
Consistent with this observation ,
PCNA immunoreactivity and
cyclin A protein expression were also increased with
bortezomib treatment . -
pubmed
We showed that the cSMAC formed by fusion of microclusters contained more
CD45 than microclusters and is a site enriched in
lysobisphosphatidic acid , a lipid involved in sorting ubiquitinated membrane proteins for degradation . -
pubmed
Calcium signaling via
TCR was blocked within 2 min by
anti - MHCp treatment and 1 min by
latrunculin - A treatment . -
pubmed
IDS epimerase does not show significant overall
amino acid sequence similarity to any other protein of known three - dimensional structure . -
pubmed
The crystal structure of this novel
epimerase has been determined by multi - wavelength diffraction to 1.5 A resolution using
selenomethionine - substituted enzyme . -
pubmed
Role of
Scarf and its binding target proteins in epidermal
calcium homeostasis . -
pubmed
The novel
Ca2 + -binding protein ,
Scarf (
skin calmodulin - related factor ) belongs to the calmodulin - like protein family and is expressed in the differentiated layers of the epidermis . -
pubmed
To further understand the functions of
Scarf in epidermis in vivo , we altered the epidermal
Ca2 + gradient by acute barrier disruption . -
pubmed
The expression of
Scarf ,
annexins ,
calreticulin , and
ERp72 were up - regulated by
Ca2 + gradient disruption , whereas the expression of
14 - 3 - 3s and
nucleolin was reduced . -
pubmed
Because annexins ,
calreticulin , and
ERp72 have been implicated in
Ca2 + -induced cellular trafficking , including the secretion of lamellar bodies and
Ca2 + homeostasis , we propose that the interaction of
Scarf with these proteins might be crucial in the process of barrier restoration . -
pubmed
Our results suggest that
Scarf plays a critical role as a
Ca2 + sensor , potentially regulating the function of its binding target proteins in a
Ca2 + -dependent manner in the process of restoration of epidermal
Ca2 + gradient as well as during epidermal barrier formation . -
pubmed
Discovery and structure - activity relationships of new steroidal compounds bearing a
carboxy - terminal side chain as
androgen receptor pure antagonists . -
pubmed
Lead optimization of
CH4892280 ( 4 ) , an
androgen receptor (
AR ) pure antagonist , was investigated . -
pubmed
Twenty micrometer sections were stained by
hematoxylin and eosin technique for gross histopathological analysis and immunolabed for neutrophils ( anti - mbs-1 ) , activated macrophages / microglia ( anti - ed1 ) , astrocytes ( anti -
gfap ) , damaged axons ( anti -
betaapp ) and
myelin basic protein (
MBP ) . -
pubmed
In a new model , with
adiponectin as a dependent variable , the
HDL cholesterol levels remained in the model ( F = 8.46 ; p & lt ; 0.05 ) , with an increase of 5.38 ( 95 % CI 1.7 - 9.1 ) ng / ml of
adiponectin with each 1 mg / dl of
HDL cholesterol . -
pubmed
Oxamate , an inhibitor of
lactate dehydrogenase (
LDH ) , abolished mitochondrial
lactate consumption . -
pubmed
The presence of
estrogen receptors (
ERs ) , as detected by immunohistochemistry ( IHC ) , is a weak prognostic marker of clinical outcome in
breast cancer , but a strong predictive marker for response , for example , to
tamoxifen - based therapy . -
pubmed
Breast cancers with
human epidermal receptor protein-2 (
c - erbB-2 ;
HER2 ) alterations are critical to identify because such
tumors require unique treatment , including the use of targeted therapies such as
trastuzumab . -
pubmed
faecalis in a synthetic medium with
ethanolamine was abolished in the response regulator
RR17 mutant
strain . -
pubmed
Ethanolamine induced a 15 - fold increase in the rate of autophosphorylation in vitro of the
HK17 sensor
histidine kinase , indicating that this is the ligand recognized by the sensor domain of the
kinase . -
pubmed
In response to : Benefit of
neoral C2 monitoring in de novo cardiac transplant recipients receiving
basiliximab induction . -
pubmed
We have examined interactions between
mitoxantrone (
MXT ) and DNA duplexes or triplexes with different base compositions by using electrospray ionization mass spectrometry ( ESI -
MS ) , respectively . -
pubmed
Moreover , no sequence selectivity of
MXT to different DNA duplexes was found while
MXT showed greater affinity to the triplexes that have adjacent
TAT or C(+)GC sequences . -
pubmed
MEASUREMENT : Maximal isometric voluntary contraction strength of the lower limb and hand with computerized dynamometers , serum
25OHD and
parathyroid hormone concentration . -
pubmed
F-18 FDG PET /
CT findings of a case of
complete hydatidiform mole . -
pubmed
The interface essentially acts as a
proton pump , allowing
hydrogen evolution by directly using the aqueous proton . -
pubmed
Reentry was easily induced by rapid pacing in cocultures ; treatment with
lidocaine , a
Na (+ ) channel blocker , significantly decreased reentry rate and CV , increased reentry path length and terminated 30 % of reentrant
arrhythmias ( n=18 ) . -
pubmed
In contrast ,
nitrendipine , an
L - type Ca ( 2 + ) channel blocker terminated 100 % of reentry episodes while increasing reentry cycle length and path length and decreasing reentry CV ( n=16 ) . -
pubmed
Reentry termination by
L - type Ca ( 2 + ) channel , but not
Na (+ ) channel , blockers suggests a greater
Ca ( 2 +)-dependence of propagation . -
pubmed
Glutamate -
aspartate transporter reduction in Bergmann glia by mutant
Atx1 and vulnerability of Purkinje cell to
glutamate are both strengthened by
Maxer knockdown in Bergmann glia , whereas
Maxer overexpression rescues them . -
pubmed
International reproducibility of single breathhold
T2 * MR for cardiac and liver
iron assessment among five
thalassemia centers . -
pubmed
In this work an LC - DAD - ESI -
MS /
MS instrument was used to develop a new tandem
MS data acquisition strategy for
anthocyanin characterization which was subsequently compared to more conventional measurements . -
pubmed
It has been shown that the newly developed strategy , multiple reaction monitoring - initiated
anthocyanin characterization (
MIAC ) , can successfully be used in
anthocyanin analysis and has various advantages compared to some more traditional measurements , such as enhanced selectivity , better signal - to - noise ratio and simplified data evaluation . -
pubmed
The plasma concentrations of
nitric oxide (
NO ) and
tumor necrosis factor - alpha (
TNF - alpha ) were measured using the Griess method and bioassay , respectively . -
pubmed
Expression of both proviruses was restricted to
androgen - responsive
prostate cancer cell lines and LTRs sequences containing
steroid hormone - responsive elements bound the
androgen receptor and conferred
androgen responsiveness to reporter constructs . -
pubmed
The
NOS-2 was only present in endothelium - denuded aortic rings , as demonstrated by immunoblot with
lactone treated rings . -
pubmed
Both
nitric oxide produced by
NOS-2 and
K + channels are involved in the relaxant effect of the
lactone . -
pubmed
Data collected included the underlying disease , basic hematological and biochemical blood test results , APACHE
II scores and serum
25 - hydroxyvitamin D [
25 ( OH ) D ] levels . -
pubmed
In addition , we show that acute exposure to
NGF , via activation of
TrkA , attenuates
GABA ( A ) receptor - mediated inhibitory synaptic currents and reduces sensitivity to exogenously applied
GABA . -
pubmed
These acute actions of
NGF display cell type and functional selectivity insofar as ( 1 ) they were found in cholinergic , but not GABAergic ,
MS / DB neurons , and ( 2 )
glutamate - mediated excitatory synaptic activity as well as
AMPA - activated current responses were unaffected . -
pubmed
Plasma membrane calcium ATPase 2 (
PMCA2 ) , an important
calcium (
Ca ( 2 + ) ) extrusion pump that interacts with synaptic signaling complexes , is most abundantly expressed in PCs compared to other neurons . -
pubmed
We focused on
Ca ( 2 + ) signaling and the expression of
glutamate receptors which play a key role in PC function including synaptic plasticity . -
pubmed
We found that the amplitude of depolarization and
2 - amino-3 -( 5 - methyl-3 - oxo-1,2 - oxazol-4 - yl ) propanoic acid receptor ( AMPAR)-mediated
Ca ( 2 + ) transients are significantly higher in cultured
PMCA2(+/- ) PCs than in
PMCA2(+/+ ) PCs . -
pubmed
This is due to increased
Ca ( 2 + ) influx , since
P / Q type voltage - gated Ca(2 + ) channel ( VGCC ) expression was more pronounced in PCs and cerebella of PMCA2(+/- ) mice and VGCC blockade prevented the elevation in amplitude . -
pubmed
Vacuolar
CAX1 and
CAX3 influence
auxin transport in guard cells via regulation of apoplastic pH. -
pubmed
CATION EXCHANGERs
CAX1 and
CAX3 are vacuolar
ion transporters involved in
ion homeostasis in plants . -
pubmed
Widely expressed in the plant , they mediate
calcium transport from the cytosol to the
vacuole lumen using the proton gradient across the
tonoplast . -
pubmed
Here , we report an unexpected role of
CAX1 and
CAX3 in regulating apoplastic pH and describe how they contribute to
auxin transport using the guard cell 's response as readout of hormone signaling and cross talk . -
pubmed
We show that
indole-3 - acetic acid (
IAA ) inhibition of
abscisic acid (
ABA)-induced stomatal closure is impaired in
cax1 ,
cax3 , and
cax1 /
cax3 . -
pubmed
Both
ethylene and
1 - naphthalene acetic acid inhibited
ABA - triggered stomatal closure in
cax1 ,
cax3 , and
cax1 /
cax3 , suggesting that auxin signaling cascades were functional and that a defect in
IAA transport caused the phenotype of the
cax mutants . -
pubmed
We also found that
cax1 /
cax3 mutants have a higher apoplastic pH than the wild type , further supporting the hypothesis that there is a defect in
IAA import in the
cax mutants . -
pubmed
Accordingly , we were able to fully restore
IAA inhibition of
ABA - induced stomatal closure in
cax1 ,
cax3 , and
cax1 /
cax3 when stomatal movement assays were carried out at a lower extracellular pH. -
pubmed
Consistent with a role for auxin in vascular specification , the
vascular defects in the
icu13 mutant were accompanied by reduced expression of auxin transport and
auxin perception markers in provascular cells . -
pubmed
Achiral
pyrazinone - based inhibitors of the
hepatitis C virus NS3 protease and drug - resistant variants with elongated substituents directed toward the
S2 pocket . -
pubmed
Structure - activity relationship data and molecular modeling support occupancy of the
S2 pocket from elongated R(6 ) substituents on the
2 ( 1H )- pyrazinone core and several inhibitors with improved inhibitory potency down to Ki = 0.11 muM were identified . -
pubmed
RESULTS : The addition of the crystalline
amino acids rendered a dose independent three - fold increase of the
FPF . -
pubmed
The studied
enzyme does not deaminate
histamine and
putrescine and is not sensitive to 10(-2 ) M
semicarbaside . -
pubmed
Moreover , overexpression of miR-382 significantly attenuated
alcohol - induced up - regulation of
DRD1 and
DeltaFosB , decreased voluntary intake of and preference for
alcohol and inhibited the
DRD1 - induced action potential responses . -
pubmed
A variety of
fatty acid esters are now being produced commercially using immobilized
lipase in nonaqueous solvents . -
pubmed
Here , we identify
TRPP2 , a member of the
transient receptor potential ( TRP ) superfamily of channel proteins encoded by the
pkd2 gene , as a central component of
Ca ( 2 + ) signaling in the kidney field . -
pubmed
TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular
Ca ( 2 + ) entry . -
pubmed
We further show that inhibition of
Ca ( 2 + ) signaling with an inducible
Ca ( 2 + ) chelator also causes downregulation of
pax8 , and that
pkd2 knockdown results in a severe inhibition of
Ca ( 2 + ) transients in kidney field explants . -
pubmed
Finally , we show that disruption of RA results both in an inhibition of intracellular
Ca ( 2 + ) signaling and of
TRPP2 incorporation into the plasma membrane of kidney field cells . -
pubmed
We propose that
TRPP2 - dependent
Ca ( 2 + ) signaling is a key component of
pax8 regulation in the kidney field downstream of RA - mediated non - transcriptional control of
TRPP2 . -
pubmed
A new page on the road book of inorganic
mercury in fish body - tissue distribution and elimination following waterborne exposure and
post - exposure periods . -
pubmed
RESULTS :
CNP reduced histological
renal tubular damage and apoptosis induced by
cisplatin and suppressed plasma blood
urea nitrogen and
creatinine levels , which were elevated by
cisplatin administration . -
pubmed
CNP treatment decreased the expression of
kidney injury molecule-1 and
monocyte chemoattractant protein-1 , which were elevated in the kidney by
cisplatin administration . -
pubmed
CNP treatment attenuated the decrease in
GC - B expression in
cisplatin - induced
kidney injury . -
pubmed
CONCLUSIONS : The present study is the first to show that
CNP inhibits
nephrotoxicity and
kidney cell damage induced by
cisplatin . -
pubmed
The mechanism of action may involve down - regulation of inflammatory
cytokine expression in
cisplatin - induced
kidney injury and attenuation of apoptosis in renal tubular cells . -
pubmed
Osteomodulin (
OMD ) is a member of the small
leucine - rich repeat proteoglycan family , which is involved in the organization of the extracellular matrix . -
pubmed
However , the potency of bacteriocin was susceptible to digestion with
Proteinase K ,
Pepsin ,
SDS ,
EDTA and
Urea . -
pubmed
Myeloperoxidase ,
tumor necrosis factor a ,
interleukin 1beta ,
PGE2 , LTB4 ,
cPLA2 ,
COX-1 , and
COX-2 levels were estimated by enzyme - linked immunosorbent assay or Western blot analysis . -
pubmed
RESULTS :
PMK - S005 significantly attenuated the
ethanol - induced
gastric damage ; it reduced mucosal inflammatory
cytokine production and increased mucus levels . -
pubmed
The expression levels of
cPLA2 ,
COX-1 , and
COX-2 were decreased by
PMK - S005 . -
pubmed
A novel
hirudin isoform 3 mimetic peptide , named peptide
S2 , has been prepared by introduction of a
stearic acid modification . -
pubmed
Peptide
S2 exhibited superior inhibitory activity to hirulog-1 (
Bivariludin ) and showed significantly higher anticoagulant potency in vivo . -
pubmed
GMP synthetase (
GMPS ) , a key enzyme in the
purine biosynthetic pathway performs catalysis through a coordinated process across two catalytic pockets for which the mechanism remains unclear . -
pubmed
Efficacy and mechanism of action of the
tyrosine kinase inhibitors
gefitinib ,
lapatinib and
neratinib in the treatment of
HER2 - positive
breast cancer : preclinical and clinical evidence . -
pubmed
This review focuses on the effects of three TKIs mostly studied in
HER2- positive
breast cancer ,
lapatinib ,
gefitinib and
neratinib . -
pubmed
In this work ,
gamma - mercaptopropyltrimethoxysilane (
gamma - MPTS ) modified
Fe3O4@
SiO2 magnetic nanoparticles ( MNPs ) was successfully prepared , and characterized by Fourier transform infrared spectrometer ( FT - IR ) , Transmission electron microscope (
TEM ) and Vibrating sample magnetometer ( VSM ) . -
pubmed
The sorption performance of the prepared
Fe3O4@
SiO2@gamma -
MPTS MNPs towards
methylmercury (
CH3Hg (+ ) ) and inorganic
mercury (
Hg ( 2 + ) ) was investigated . -
pubmed
It was found that
CH3Hg (+ ) and
Hg ( 2 + ) could be simultaneously retained on the prepared
Fe3O4@
SiO2@gamma -
MPTS MNPs , and the quantitative elution of
CH3Hg (+ ) and total
mercury ( THg ) was achieved by using 1.5 mol L(-1 )
HCl containing 0.01 % and 3 %
thiourea ( m / v ) , respectively . -
pubmed
Based on the above facts , a method of magnetic solid phase extraction ( MSPE ) combined with inductively coupled plasma mass spectrometry ( ICP -
MS ) was developed for the speciation of
CH3Hg (+ ) and
Hg ( 2 + ) . -
pubmed
The interrelationship between oxygenated
hemoglobin , deoxygenated
hemoglobin , total
hemoglobin and cerebral
oxygen exchange are related to initial dips . -
pubmed
In the
iron - depleted condition , overexpression of
iron homeostasis genes was observed independent of
codY deletion ; however , transcription of siderophore and
amino acid biosynthesis genes was
CodY dependent . -
pubmed
Although
CodY has a significant regulatory role in central metabolism and the
carbon overflow pathway , metabolism - associated genes exhibited
CodY - independent expression patterns under
glucose starvation . -
pubmed
Genes that were differentially expressed in response to
CO2 availability showed
CodY - dependent regulation , though their maximal expression did require a supply of
CO2 /
bicarbonate . -
pubmed
Baduanjin users also experienced a significant elevation in the serum
melatonin levels but showed no significant differences in the Self - Rating
Anxiety Scale (
SAS ) score . -
pubmed
In this paper , two high atomic number filter materials are studied , namely ,
gadolinium (
Gd ) and
erbium (
Er ) , and compared with
Al and
Cu filters currently used in breast
CT systems . -
pubmed
It was observed that spectral shaping using
Er and
Gd filters substantially increased the dose efficiency ( defined as signal - to - noise ratio of the
CHO divided by mean glandular dose ) as compared with kilovolt peak and filter settings used in commercial and prototype breast
CT systems . -
pubmed
The
endothelial nitric oxide synthase cofactor
tetrahydrobiopterin shields the remote myocardium from apoptosis after experimental
myocardial infarction in vivo . -
pubmed
Excess activation of
endothelial nitric oxide synthase (
eNOS ) increases its uncoupling potential and results in nitrosative stress via formation of
peroxynitrite . -
pubmed
Notably , pretreatment with the
eNOS cofactor
BH4 reduced
eNOS activation , prevented excess protein nitrosylation , blunted apoptosis induction , facilitated anti - apoptotic signalling , and eventually prevented apoptosis execution . -
pubmed
Evidence is presented that pretreatment with the
eNOS cofactor
BH4 resulted in less nitrosative stress and weakened apoptotic processes , although the stabilisers contained did participate in this phenomenon . -
pubmed
Then , after bleaching labial enamel surfaces of 20 teeth with 15 %
carbamide peroxide 6 hours a day for 5 days , they were randomly divided into two groups :
A1 and
A2 ( n = 10 ) , depending upon whether or not they are treated with antioxidant . -
pubmed
Labial enamel surfaces of the remaining 20 teeth were bleached with 38 %
hydrogen peroxide before being randomly divided into groups
B1 and B2 ( n = 10 ) , again depending on whether or not the antioxidant was used in their treatment . -
pubmed
Psilocybin with psychological support improves emotional
face recognition in treatment - resistant
depression . -
pubmed
CONCLUSIONS :
Psilocybin with psychological support appears to improve processing of emotional
faces in treatment - resistant
depression , and this correlates with reduced
anhedonia . -
pubmed
Design : In a separate study , oral
glucose tolerance tests , a 45 - minute bout of exercise , lipid infusions , and euglycemic - hyperinsulinemic
clamps ( EHCs ) were performed to investigate the association of
myonectin with homeostasis model assessment of
insulin resistance ( HOMA - IR ) and
T2DM . -
pubmed
Plasma
myonectin correlated positively with waist / hip ratio , percentage of body fat ,
triglyceride , fasting blood
glucose , 2 - hour blood
glucose after
glucose overload , fasting
insulin ,
hemoglobin A1c , and HOMA - IR and negatively with the
insulin sensitivity index in all of the study population . -
pubmed
Circulating
myonectin levels were not significantly altered in response to an oral
glucose challenge or EHC . -
pubmed
In addition , acute elevated free
fatty acid levels induced by lipid infusion had no effects on circulating
myonectin . -
pubmed
GAPDH levels in the preadipocytes were not significantly different at the doses tested for the
flavonoids and exogenous
GSH . -
pubmed
However , significant ( p & lt;.05 ) decreases in
phospho -
AKT levels in cells treated with
quercetin ,
kaempferol , and
GSH at certain doses were observed compared to their respective controls . -
pubmed
AUX1 - mediated root hair
auxin influx governs
SCFTIR1 /
AFB - type
Ca2 + signaling . -
pubmed
This depolarization is strongly impaired in
aux1 mutants , indicating that
AUX1 is the major transporter for
auxin uptake in root hairs . -
pubmed
AUX1 - mediated
IAA transport , as well as
IAA- triggered
calcium signals , are blocked by treatment with the
SCFTIR1 /
AFB - inhibitor
auxinole . -
pubmed
Our study reveals that the
AUX1 transporter , the
SCFTIR1 /
AFB receptor and the
CNGC14 Ca2 + channel , mediate fast
auxin signaling in roots . -
pubmed
Furthermore , pharmacological inhibition of the endogenous
P2Y6 receptor activity by the potently selective
P2Y6R antagonist ,
MRS 2578 , greatly up - regulated pro - inflammatory cytokine interleukin ( IL)-1beta ,
IL-6 and
TNF - alpha gene expression in PBL cells treated with
UDP . -
pubmed
[ Imaging of parathyroid
adenomas with F-18
choline PET -
CT ] . -
pubmed
Recently ,
F-18 choline positron emission tomography computed tomography (
choline PET /
CT ) has been shown promising results for the diagnostic work up of
primary hyperparathyroidism (
pHPT ) suggesting superiority over conventional scintigraphy using
Tc99 m sestamibi based protocols using planar dual - phase imaging , SPECT or SPECT /
CT . -
pubmed
METHODS : This review presents the results of
F-18 choline PET /
CT on the basis of a literature search using the keywords " primary
hyperparathyroidism and
choline " , " primary
hyperparathyroidism and PET " , " parathyroid
adenoma and
choline " und " parathyroid
adenoma and PET " . -
pubmed
RESULTS : 6 studies were identified dealing with the diagnostic impact of
choline PET /
CT . -
pubmed
Localization of single gland
adenomas can be achieved with
choline PET /
CT in 80 up to 96 % of cases . -
pubmed
Isocitrate lyase , an essential enzyme for growth on
acetate , was expressed under an
arabinose inducible promoter . -
pubmed
The autonomous downregulation of the expression is based on the gradual oxidation of the
arabinose inducer by a
glucose dehydrogenase gcd . -
pubmed
The aim of this research work was to develop a solid supersaturated self - nanoemulsifying drug delivery system of
fenofibrate (
FB ) for enhanced dissolution . -
pubmed
METHODS : FB loaded supersaturated self - nanoemulsifying preconcentrate ( superSNEP ) was prepared using
dimethyl acetamide (
DMA ) , medium chain
triglycerides (
MCT ) , and kolliphor EL . -
pubmed
Co - processed excipients (
CPE ) prepared using inorganic microporous
silica (
Neusilin US2 ,
Florite 100 , or
Aerosil 200 ) and hydrophilic polymers (
Polyvinyl alcohol , HPMC , and
Kollidon VA64 ) were evaluated for flow property , BET surface area , and adsorption capacity . -
pubmed
CPE prepared using
PVA and
Florite 100 ( 1:1 weight ratio ) showed the highest adsorption capacity ( 1 mL / g ) and complete release of oil as depicted by fluorescence study . -
pubmed
Pro - inflammatory
cytokines concentrations were also decreased by
DMF treatment . -
pubmed
DMF downregulated the expression of inflammatory (
NF - kB ) and apoptotic (
Bax ,
Caspase-3 ) genes and protein . -
pubmed
DMF treatment increased the gene expression of
Nrf2 ,
HO-1 ,
NQO1 ,
Bcl-2 and protein expression of
Nrf2 and
Bcl2 . -
pubmed
To expand the application of double network hydrogel in water treatment , in this study , eco - friendly physically cross - linked double network polymer hydrogel beads (
DAP ) are prepared and studied in depth on the mechanism of
Methylene Blue ( MB ) adsorption ; and then the polymer hydrogels are further functionalized by inorganic materials . -
pubmed
In general , the
ECD of a protein is involved in protein - protein or protein -
carbohydrate interactions , whereas the ICD is important for intracellular signaling . -
pubmed
We previously reported that
GPNMB contributes to the initiation and malignant progression of
breast cancer through the hemi - immunoreceptor
tyrosine - based activation motif ( hemITAM ) in its ICD . -
pubmed
Furthermore , we showed that the
tyrosine residue in
hemITAM is involved in induction of the stem - like properties of
breast cancer cells . -
pubmed
MOF exhibited better removal rates than commercial powder activated
carbon ( PAC ) , considering pseudo - second order kinetic model . -
pubmed
To confirm the practicality of
MOF adsorption , we carried out regeneration tests with four adsorption and desorption cycles using
acetone as a cleaning solution . -
pubmed
Serum metabonomics studies were performed by UPLC - Q -
TOF -
MS to reveal the dose - dependent biochemical disturbances caused by
PMP . -
pubmed
In addition ,
carbachol - induced contraction was reduced in isolated detrusor strips from the
L - NAME group , and bladder expression of
HIF-1 and
connexin 43 showed upregulation . -
pubmed
METHODS : We performed a bibliographic research on PUBMED of all studies investigating the use of
IV valproate as a treatment of acute
mania in BD - I. -
pubmed
RESULTS : Overall , the results suggest that
IV valproate as a loading therapy is an efficacious , safe and well tolerated treatment for
manic episodes , and it is comparable to the oral loading regimen . -
pubmed
CONCLUSIONS :
IV valproate may represent a valid option for the management of acute
mania , with comparable effects in terms of efficacy and safety to the oral
valproate . -
pubmed
However , larger and more homogeneous studies are warranted in order to collect more precise information on the beneficial effect of
IV valproate . -
pubmed
The control group did not receive any kind of intervention , one group received functional electrical stimulation of lower limb muscles ( FES group ) , another group practiced Tai Chi exercise (
TCE group ) , and another received functional electrical stimulation of lower limb muscles and practiced Tai Chi exercise (
FES & amp ;
TCE group ) . -
pubmed
Compared to the control group , only
FES group had increased Kansas City
Cardiomyopathy Questionnaire ( KCCQ ) score ( P<0.0001 , q=9.06 ) , only the
TCE group had decreased heart rate ( P<0.0001 , q=5.72 ) , and decreased peak
oxygen consumption was reported in the
TCE group ( P<0.0001 , q=9.15 ) and
FES & amp ;
TCE group ( P<0.0001 , q=10.69 ) . -
pubmed
We measure
post -
shock temperature and pressure in experiments with nonreacting binary mixtures of
sulfur hexafluoride and
helium ( two dramatically disparate gases ) and show that neither model can accurately predict the observed values , on time scales much longer than that of the
shock front passage , due to the models ' implicit assumptions about mixture behavior on the molecular level . -
pubmed
However , enthalpy and Tp were higher in the sample without HIU analyzed after
OM due to the migration of low melting point
triacylglycerols out of the crystalline network . -
pubmed
This study introduces for the first time , a new processing technology to reduce
OM in a
palm kernel fat with high content of
saturated fatty acids that is commonly used in confectionery applications . -
pubmed
Lactate and
BDNF : Key Mediators of Exercise Induced Neuroplasticity ? -
pubmed
Current research has shown that ( i ) increased peripheral
lactate levels ( following high intensity exercise ) are associated with increased peripheral
BDNF levels , ( ii )
lactate infusion at
rest can increase peripheral and central
BDNF levels and ( iii )
lactate plays a very complex role in the brain 's metabolism . -
pubmed
In this review , we summarize the role and relationship of
lactate and
BDNF in exercise induced neuroplasticity . -
pubmed
Conclusions : This review article highlights the role of fundus
fluorescein angiography (
FA ) ,
indocyanine green angiography ( ICG ) , optical coherence tomography ( OCT ) , and optical coherence tomography angiography ( OCTA ) in the diagnosis and management of
ocular sarcoidosis . -
pubmed
This review article highlights the role of fundus
fluorescein angiography (
FA ) ,
indocyanine green angiography ( ICG ) , optical coherence tomography ( OCT ) , and optical coherence tomography angiography ( OCTA ) in the diagnosis and management of
ocular sarcoidosis . -
pubmed
PSMA - targeted Radiotracers versus
18F Fluciclovine for the Detection of
Prostate Cancer Biochemical Recurrence after Definitive Therapy : A Systematic Review and Meta - Analysis . -
pubmed
The comparative performance between
fluciclovine and
gallium 68 or 18F
prostate - specific membrane antigen (
PSMA ) PET /
CT , a newer examination , is unclear . -
pubmed
Studies of
fluciclovine PET /
CT or
PSMA PET /
CT in biochemical
recurrence were identified . -
pubmed
Pooled detection rates for
PSMA and
fluciclovine were 45 % ( 95 % CI : 38 % , 52 % ) and 37 % ( 95 % CI : 25 % , 49 % ) , respectively , for a
PSA level less than 0.5 ng / mL ( P = .46 ) ; 59 % ( 95 % CI : 52 % , 66 % ) and 48 % ( 95 % CI : 34 % , 61 % ) for a
PSA level of 0.5 - 0.9 ng / mL ( P = .19 ) ; and 80 % ( 95 % CI : 75 % , 85 % ) and 62 % ( 95 % CI : 54 % , 70 % ) for a
PSA level of 1.0 - 1.9 ng / mL ( P = .01 ) . -
pubmed
The Association of
ARMC5 with the
Renin -
Angiotensin -
Aldosterone System , Blood Pressure and Glycemia in African Americans . -
pubmed
We examined the association of
ARMC5 variants with
aldosterone ,
plasma renin activity (
renin ) , blood pressure ,
glucose and
hemoglobin A1c ( A1c ) in community - dwelling
AAs . -
pubmed
Kernel machine method was used to perform a single joint test to analyze for an overall association between the phenotypes of interest (
aldosterone ,
renin , systolic and diastolic blood pressure [
SBP ,
DBP ] ,
glucose and A1c ) and the
ARMC5 single nucleotide variants ( SNVs ) adjusted for age , sex ,
BMI , and medications ; followed by Baysian Lasso methodology to identify sets of SNVs in terms of associated haplotypes with specific phenotypes . -
pubmed
Haplotype TCGCC ( ch16:31476015 - 31476093 ) was negatively associated , whereas haplotype CCCCTTGCG ( ch16:31477195 - 31477460 ) was positively associated with
SBP ,
DBP , and
glucose . -
pubmed
CONCLUSIONS :
ARMC5 variants are associated with
aldosterone , PRA , blood pressure , fasting
glucose and A1c in community - dwelling
AAs , suggesting germline mutations in
ARMC5 may underlie
cardiometabolic disease in
AAs . -
pubmed
The linear relationship observed between the thermodynamic force and the thermodynamic flow allowed to establish that
MSC - laden in
collagen scaffolds were in a near - equilibrium stationary state ( affinity < < RT ) , MSCs were also seeded in solid
collagen scaffolds functionalized with the tripeptide
Arg - Gly - Asp ( RGD ) . -
pubmed
Background and Aims :
Mirtazapine is an atypical antidepressant with antagonist activity for
serotonin and histamine receptors . -
pubmed
Large - scale population screenings are not feasible by applying laborious oral
glucose tolerance tests , but using fasting blood
glucose ( FPG ) and
glycated hemoglobin (
HbA1c ) , a considerable number of diagnoses are missed . -
pubmed
Physical , mechanical parameters (
weight loss , decay rate , colour index and firmness ) , phytochemical contents (
ascorbic acid , acidity , soluble solids concentration , titratable acidity , and repining index ) , phenolic enzymes (
peroxidase and
polyphenoloxidase ) , pigments (
anthocyanin ) and microbiological analysis ( mesophilic aerobic , yeasts and molds populations ) were detected every other day until the end of the experiment . -
pubmed
( B / CH / Nano -
TiO2 ) reported a gradual increase in
polyphenoloxidase and
peroxidase enzyme activities ( 659.45 U / min g ) and ( 20.39 U / min g ) , respectively . -
pubmed
Since the
ET plasmid - negative substrains still made
ET , it was postulated that this residual
toxin was made from chromosomal genes . -
pubmed
Treatment of cleared lysates from
strain UT0002 with
ethidium bromide ,
Pronase , or
sodium dodecyl sulfate , but not heat at 60 C , induced conversion of the 56S closed circular ET plasmid to a 38S open circular form as determined after centrifugation on 5 to 20 % neutral
sucrose gradients . -
pubmed
The binding rate of
pyridoxal ' 5 - phosphate (
Pxa - P ) to
apotryptophanase and the dissociation rate of the coenzyme from
holotryptophanase were able to be determined by following the
enzyme activity in continuous flow reactions on a column of immobilized
tryptophanase . -
pubmed
Immobilized
tryptophanase freed of coenzyme restored rapidly its original activity , when the assay mixture containing a given concentration of substrate and
Pxa - P was passed through the immobilized
enzyme column . -
pubmed
This means that
Schiff base formation between
Pxa - P and
tryptophan in the assay mixture has no effect on the binding of
Pxa - P to
apoenzyme . -
pubmed
It was found by an enzymatic study that
riboflavin (
FR ) was apparently converted via
SF2 to
SF1 , and that
SF2 was a direct precursor of
SF1 . -
pubmed
The
carboxylic group in
SF1 and the
aldehyde group in
SF2 were detected by
spot tests . -
pubmed
Elemental analyses and the spectroscopic analyses of the crystalline
SF1 supported the existence of
carboxylic group in the terminal of its
ribityl moiety . -
pubmed
From these experimental results ,
schizoflavins were revealed to be oxidation products of
FR that C-5 ' site of its
ribityl moiety is oxidized . -
pubmed
Consequently ,
SF1 and
SF2 were identified as
7,8 - dimethyl-10 -( 2,3,4 - trihydroxy-4 - carboxybutyl ) isoalloxazine and
7,8 - dimethyl-10 -( 2,3 , 4 - trihydroxy-4 - formylbutyl ) isoalloxazine , respectively . -
pubmed
Thus , we postulated that
acetylcholine (
ACh ) stimulates bronchial epithelial cells ( BEC ) to release neutrophil and monocyte chemotactic activity (
NCA and MCA ) . -
pubmed
BEC released
NCA and MCA in response to
ACh in a dose - dependent and time - dependent manner . -
pubmed
The release of
NCA and
MCA was inhibited by the
lipoxygenase inhibitors ,
nordihydroguaiaretic acid and
diethylcarbamazine . -
pubmed
To determine whether smoke might activate BEC to release
NCA by stimulating
nicotinic ACh receptors , we further characterized the
ACh receptors , using
nicotine and nicotinic and
muscarinic receptor antagonists . -
pubmed
Nicotine , the
nicotinic receptor antagonist
d - tubocurarine , and the
M2 receptor antagonist
gallamine did not modulate the release of
NCA in response to
ACh . -
pubmed
In contrast ,
atropine and the
M1 receptor antagonist ,
pirenzepine , inhibited the release of
NCA . -
pubmed
These data demonstrate that
ACh stimulates BEC to release
lipoxygenase - derived
NCA and
MCA through the
muscarinic receptor . -
pubmed
The deduced
amino acid sequence is similar to that of
human brain calcineurin beta subunit with respect to containing four putative
calcium binding sites . -
pubmed
However , distinct differences were found : 1 ) The cloned cDNA had six
amino acids polypeptide tail at carboxy - terminal which is absent in
human brain calcineurin beta subunit . -
pubmed
The ionization states of the
5 '- phosphate group in the various coenzyme forms bound to mitochondrial
aspartate aminotransferase . -
pubmed
We have carried out a Fourier transform infrared spectroscopic study of mitochondrial
aspartate aminotransferase in the spectral region where
phosphate monoesters give rise to absorption . -
pubmed
The integrated intensity of this band in several
enzyme forms (
pyridoxal phosphate ,
pyridoxamine phosphate , and
sodium borohydride - reduced ,
pyridoxyl phosphate form ) does not change with pH in the range 5 - 9 . -
pubmed
The integrated intensity value of this infrared band for the
PLP enzyme form before and after reduction with
sodium borohydride is close to that given by free
PLP at pH 8 - 9 . -
pubmed
These results are taken as evidence that in the active site of mitochondrial
aspartate aminotransferase the
5 '- phosphate group of
PLP remains mostly dianionic even at a pH near 5 . -
pubmed
Thus , it is suggested that the chemical shift changes associated with pH titrations of various
PLP forms reported in a previous
31P NMR study of this
enzyme [ M. E. Mattingly , J. R. Mattingly , and M. Martinez - Carrion ( 1982 ) J. Biol . -
pubmed
Since no chemical shift changes were observed in pH titrations of the
PMP forms ( lacking an ionizable internal
aldimine ) of this
isozyme , the
Schiff base between
PLP and
Lys-258 at the active site is the most likely candidate for the ionizing group influencing the
phosphorus chemical shift in this
enzyme . -
pubmed
The
MAC - reducing effect of
L - DOPA was attenuated by selective antagonism of the
D2 dopamine receptor with
YM-09151 - 2 while selective blockade of the
D1 dopamine receptor with
SCH-23390 did not alter
L - DOPA 's effect on the MAC for
halothane . -
pubmed
Inhibitory effects of
spermine and
spermidine on muscle
calpain II . -
pubmed
The muscle enzyme
calpain II , in contrast to muscle
calpain I , was markedly inhibited by millimolar concentrations of the
polyamines spermine and
spermidine . -
pubmed
These results suggest that the
polyamines may have possible regulatory effects on the in vivo activity of
calpain II enzymes . -
pubmed
Even after a 9 - h chase in the presence of
brefeldin A ,
LDL receptor 5 - 10 kDa smaller than the normal mature form was found to be stable . -
pubmed
Benazepril significantly reduced
systolic blood pressure (
SBP ) and
DBP , both supine and standing ( p less than 0.01 ) , while heart rate ( HR ) did not change . -
pubmed
These results imply that multidisciplinary treatments in combination of immuno - chemotherapy with
IFN - beta ,
IFN - gamma or vinca
alkaloids ,
hyperthermia and irradiation should be applied for advanced
renal cell carcinoma . -
pubmed
Dark adaptation increased the
calcium - independent
CaM kinase activity 2.7 times and
calcium - stimulated activity 3.9 times in membrane fractions . -
pubmed
In the cytosol , dark adaptation increased
calcium- and
calmodulin - independent
kinase activity 3.3 - fold but did not enhance
calcium- and
calmodulin - dependent activity . -
pubmed
The dose of
N - acetylcysteine was 150 mg / kg given in 15 min followed by 50 mg / kg in 4 h and 100 mg / kg over the next 16 h.
Liver damage was absent or mild on 13 occasions (
ALT greater than 500 mu / l ) and severe on 5 (
ALT less than 1000 mu / l ) . -
pubmed
Application of monoclonal antibody techniques to the study of
Na + , K +- ATPase offers a unique opportunity to investigate the molecular basis of
sodium potassium transport . -
pubmed
However , after higher doses of
clonidine ( 0.56 and 1.2 nmol ) a small and short - lasting period of behavioural and ECoG
SWS was still evident . -
pubmed
Previous studies have demonstrated that two peptides ,
substance P ( SP ) and
substance K ( SK ) , are contained in a common prohormone -- beta -
preprotachykinin . -
pubmed
This study evaluated the behavioral activity of
SK and compared it to the activities of
SP ,
bombesin ( BN ) , and structurally related peptides . -
pubmed
The specific rank order of these durations of action were : BN greater than
gastrin releasing peptide (
GRP ) =
ranatensin (
RT ) greater than
neuromedin B (
NMB ) greater than
kassinin (
KASS ) = SK = SP and ranged from dose - dependent maxima of approximately 2 min ( SP ) to approximately 100 min ( BN ) . -
pubmed
Since the hypothalamus and septum are known to be critical areas for water and
electrolyte balance , and blood pressure control , these results suggest that
ANP plays a pathophysiological role in these brain regions of SHR . -
pubmed
GCR binding is selectively inhibited by methylation of two short , symmetrically arranged clusters of
guanine residues within this site . -
pubmed
The newly synthesized
acid hydrolases acquire
Man 6 - P residues by a two - step reaction . -
pubmed
Then ,
N - acetylglucosamine residues are removed by the
N - acetylglucosaminyl phosphoglycosidase to generate the
Man 6 - P monoester , which is capable of binding the
Man 6 - P receptor . -
pubmed
Following delivery of enzyme to lysosomes , the enzymes undergo
post - lysosomal processing by
acid phosphotases , which remove the
phosphomonoester groups , and
acid proteases which reduce their size and trim off excess polypeptides . -
pubmed
Serum
type III procollagen peptide in
alcoholic liver disease and
idiopathic hemochromatosis : its relationship to
hepatic fibrosis , activity of the disease and
iron overload . -
pubmed
Our findings , therefore , weaken the diagnostic value of
sPIIIP as an index of connective tissue deposition in the liver , and suggest that , at least in
alcohol - related
liver disease and
IHC ,
hepatocellular necroinflammation influences the results . -
pubmed
SDS - PAGE examination of the microsomes revealed the induction of a
cytochrome P-450 isozyme of 53.1 kDa , but the
aniline hydroxylase and
p - nitroanisole O - demethylase activities of the 9000 g supernatant of liver were not induced . -
pubmed
It is important to note that
liver hypertrophy , microsomal
enzyme induction and the excretion of unchanged
cinnamyl anthranilate all have the same dose - threshold for their appearance . -
pubmed
Alteration of
tyrosine hydroxylase activity in the locus coeruleus after administration of
p - chlorophenylalanine . -
pubmed
Analysis of
HMG proteins by NEPHGE /
sodium dodecyl sulfate - gel electrophoresis is thus precluded in samples containing
histones . -
pubmed
At certain intervals after ovariectomy , a single administration of
delta 9 - tetrahydrocannabinol (
THC ; 50 mg / kg ) reduced plasma levels of
luteinizing hormone (
LH ) and
follicle - stimulating hormone (
FSH ) , while at other times
THC had no effect . -
pubmed
Administration of
THC significantly reduced plasma concentrations of
LH and
FSH in intact diestrous females . -
pubmed
In an additional study , in intact diestrous females injected with
testosterone propionate ( TP ; 125 micrograms ) , concommitant exposure to
THC did not affect plasma
testosterone ( T ) levels , but plasma
dihydrotestosterone (
DHT ) levels at 20 and 60 min were significantly higher in animals receiving
TP and
THC . -
pubmed
Effects of
THC on plasma
androgen levels after exogeneous treatment with
TP suggest that
THC can alter metabolism or target tissue response to gonadal
steroids . -
pubmed
Intravenous infusion of 0.7 and 2.9 pmol min-1 kg-1 produced significant elevation of plasma
gastrin , cholecystokinin - like immunoreactivity and
neurotensin . -
pubmed
It was found also to potentiate
glucose - dependent
insulin secretion . -
pubmed
Known liver function tests ( activities of
ASAT =
L - Aspartate : 2 - oxoglutarate aminotransferase ,
ALAT =
L - Alanine : 2 - oxoglutarate aminotransferase ,
GLDH =
Glutamate - dehydrogenase ,
LDH =
Lactate - dehydrogenase in serum ) are not qualified for detecting minimal histopathological
liver alterations induced by
aminophenazon . -
pubmed
The full - length cDNA as well as genomic clones contain a single open reading frame ( ORF ) of 873 bp that encodes a protein with 291
amino acids comprising the mature
narbonin polypeptide ( M(r ) ca . -
pubmed
Sequence analysis of this clone revealed a continuous unspliced transcript between the region of
UL101X and
UL102 ; the only in - frame translational stop codon is 2,619 bp downstream from the first ATG in the
message . -
pubmed
[ Clinical evaluation on the detection of
rifampicin specific
IgG ,
IgM ] . -
pubmed
The reactions of the positive serum could be inhibited significantly by the addition of
RFP ,
RFP - RSA but not by RSA . -
pubmed
Vasoconstriction of about 35 - 40 % of the maximal effect obtained with
KCl was found with mononuclear cells activated by
thrombin or complement component
C5a , but not with unactivated mononuclear cells . -
pubmed
HS-142 - 1 , a selective antagonist for
ANP biological receptors , inhibited
ANP - induced cellular
cGMP accumulation and prevented
ANP - induced inhibition of both serum - stimulated incorporation of
3H - thymidine and cell proliferation . -
pubmed
Des -[ Gln18 ,
Ser19 ,
Gly20 ,
Leu21 ,
Gly22]-ANP4 - 23 - NH2 ( C -
ANP ) failed to inhibit
3H - thymidine incorporation . -
pubmed
Oxidative regeneration and selective reduction of native
disulfide bonds in the N - terminal half - molecule of
ovotransferrin . -
pubmed
The denatured ,
disulfide - reduced form of the N - terminal half - molecule of
ovotransferrin was reoxidized with either oxidized
dithiothreitol or
GSSG and analyzed for the localization of
disulfide bonds . -
pubmed
Chemical analyses of the reoxidized proteins revealed that the
disulfide peptides corresponding to the six native protein
disulfides (
SS - I ,
SS -
II ,
SS - III ,
SS -
IV ,
SS - V , and
SS - VI ) are all regained in the reoxidized
protein . -
pubmed
The peptide recoveries from the reoxidized
proteins were , however , about half of those from the native
protein with respect to the two inner
disulfides (
SS -
IV and
SS - V ) in the kringle bridges , but all the
disulfide peptides corresponding to the remaining
disulfides (
SS - I ,
SS -
II ,
SS - III , and
SS - VI ) were recovered at almost equivalent yields in the native and reoxidized
proteins . -
pubmed
In addition , on searching for a nonnative
disulfide peptide , the two
disulfides ,
Cys171 -
Cys174 and
Cys174 -
Cys182 , which can be accounted for by mispaired bridges of
sulfhydryls in
SS -
IV and
SS - V , were detected in the
protein reoxidized with oxidized
dithiothreitol . -
pubmed
Upon
disulfide reduction of the native
protein with reduced
dithiothreitol , both
SS -
IV and
SS - V were selectively cleaved under the same buffer and temperature conditions as in the oxidative refolding . -
pubmed
The lower stabilities of the two inner
disulfide bonds in the kringle may be related to the lower recoveries of the
disulfide peptides from
SS -
IV and
SS - V and the generation of the nonnative
disulfide bonds . -
pubmed
A 20 - year - old woman from a consanguineous family in the Hunan Province of the People 's Republic of China was diagnosed as having
Glanzmann ' s thrombasthenia based on ( 1 ) nearly a lifelong history of
epistaxis ,
gum bleeding ,
petechiae , and
purpura ; ( 2 ) severe
menorrhagia resulting in
anemia and need for whole - blood transfusion ; ( 3 ) normal coagulation assays ; ( 4 ) prolonged bleeding time ; ( 5 ) absent clot retraction ; ( 6 ) decreased glass bead retention ; ( 7 ) absent
platelet aggregation in response to
adenine diphosphate ,
epinephrine , and
collagen ; and ( 8) normal initial slope of
platelet aggregation in response to
ristocetin , but with a diminished maximal extent . -
pubmed
Sequence analysis of the patient 's
GPIIIa RNA identified a G to A mutation at nucleotide 1219 , predicting a
Cys to
Tyr substitution at residue 374 . -
pubmed
Anti - pseudomonal activity of
collagen sponge with liposomal
polymyxin B. -
pubmed
An antibiotic delivery system has been developed using
collagen sponge with liposome - encapsulated
polymyxin B. -
pubmed
Wounds were dressed with
collagen sponge containing liposomal
polymyxin B or containing empty liposomes ( with
PBS ) as a control . -
pubmed
To investigate whether the differences in morphology are due to incorporation of different packaging proteins , spermatozoa
nuclear proteins were characterised by
acetic acid -
urea polyacrylamide gel electrophoresis ( PAGE ) and fractionated by reverse - phase high - pressure liquid chromatography ( HPLC ) . -
pubmed
METHODS AND RESULTS : Blood was anticoagulated alone or in combination with
citrate ,
ethylenediaminetetraacetatic acid ,
corn trypsin inhibitor ( CTI , an inhibitor of
activated factor XII ) ,
heparin ,
enoxaparin ,
recombinant tick anticoagulant peptide (
rTAP ) , or
recombinant hirudin . -
pubmed
Platelet activation in response to
adenosine diphosphate (
ADP ) or
collagen was detected by assay of
P - selectin on the platelet surface delineated by flow cytometry . -
pubmed
Although minimal activation was seen without
ADP , the fraction of platelets expressing
P - selectin in response to
ADP was greatest in blood anticoagulated with
citrate compared with
CTI and all other anticoagulants . -
pubmed
ADP - induced
platelet activation was greater in blood anticoagulated with heparin compared with an equipotent
anti - Xa concentration of
enoxaparin . -
pubmed
More variable results were seen with
collagen , but platelet activation in the presence of
citrate was greater than that with
CTI . -
pubmed
Low molecular weight protein - tyrosine phosphatase tyrosine phosphorylation by
c - Src during
platelet - derived growth factor - induced mitogenesis correlates with its subcellular targeting . -
pubmed
As a consequence of its
tyrosine phosphorylation ,
LMW - PTP significantly increases its catalytic activity . -
pubmed
On the other hand we have now demonstrated that the cytoskeleton - associated
LMW - PTP acts specifically on a few not yet identified proteins that become
tyrosine - phosphorylated in response to the
PDGF receptor activation . -
pubmed
The cytoplasmic LMW -
PTP pool exerts its action within a few minutes from
PDGF receptor activation ( short term action ) , while
tyrosine phosphorylation of cytoskeleton - associated LMW -
PTP lasts for more than 40 min ( long term action ) . -
pubmed
To establish the subcellular localization of the
PS1 regulation of
APP processing to
Abeta , fibroblasts from
PS1 wild - type ( wt ) or knockout ( KO ) embryos as well as Chinese hamster ovary ( CHO ) cells stably transfected with wt or mutant
PS1 were subjected to subcellular fractionation on discontinuous
Iodixanol gradients . -
pubmed
Bilirubin , formed by activation of
heme oxygenase-2 , protects neurons against oxidative stress
injury . -
pubmed
Heme oxygenase (
HO ) catalyzes the conversion of
heme to
carbon monoxide ,
iron , and
biliverdin , which is immediately reduced to
bilirubin (
BR ) . -
pubmed
Two
HO active isozymes exist :
HO1 , an inducible
heat shock protein , and
HO2 , which is constitutive and highly concentrated in neurons . -
pubmed
We demonstrate a neuroprotective role for
BR formed from
HO2 . -
pubmed
Neurotoxicity elicited by
hydrogen peroxide in hippocampal and cortical neuronal cultures is prevented by the
phorbol ester ,
phorbol 12 - myristate 13 - acetate (
PMA ) via stimulation of
protein kinase C. -
pubmed
One gene out of about 1000 genes analyzed , identified as
alpha - tropomyosin , showed an increased transcription level caused by
alpha - tocopherol treatment . -
pubmed
The aetiology of this development may be related to the patient 's
HLA status or possibly to his treatment with the immunosuppressive agent
cyclophosphamide ; it is also possible that it is coincidental . -
pubmed
All three RNA structures were subsequently subjected to twin refinement in space group
P1 , using anisotropic thermal displacement parameters at resolutions of 1.16 , 1.23 and 1.4 A for
ALA(wt ) ,
ALA(I ) and
ALA(C70 ) , respectively . -
pubmed
Alternatively , the structure of
ALA(wt ) was refined in space group
C2 assuming twofold disorder of the molecular orientation . -
pubmed
Previous studies using pharmacological and behavioral approaches suggested that
MDMA treatment leads to desensitization of
5 - HT1B receptors . -
pubmed
[ 3H ]- citalopram binding to
serotonin transporters in hippocampus was reduced in the high dose protocol , indicating degeneration of forebrain serotonergic fibers . -
pubmed
[ 125I ]- Iodocyanopindolol binding to
5 - HT1B sites in hippocampus was also not changed . -
pubmed
In Experiment
II , high dose
MDMA had no effect on
5 - HT1B mRNA in any brain region either 1 or 14 days after treatment . -
pubmed
These results suggest that previously described changes in
5 - HT1B function following
MDMA treatment involve only posttranscriptional changes in receptor regulation and do not alter
5 - HT1B mRNA levels . -
pubmed
Ramipril uniformly decreased deltaGFR and incidence of
ESRD in women with either
DD ( -39 % and - 100 % ) or
II + ID ( -71 % and -82 % ) genotype , and in men ( -25 % and -50 % ) with the DD genotype , but had no beneficial effect in men with the
II + ID genotype ( +18 % and +34 % ) . -
pubmed
Thus , the relative risk of events (
ESRD ) between conventional and
ramipril - treated men was higher in subjects with the DD genotype ( 1.85 ; 0.69 to 4.94 ) and lower in those with the
II + /- ID genotype ( 0.71 ; 0.28 to 1.80 ) . -
pubmed
Of note , the
ACE genotype - related effect of
ramipril was still evident even after correction for the above potentially confounding factors ( adjusted RR [ 95 % CI ] :
DD , 2.52 [ 0.83 to 7.63 ] , P = 0.10 ;
II + ID , 0.35 [ 0.12 to 1.01 ] , P = 0.05 ) . -
pubmed
Caveolin-1 is an integral membrane protein of caveolae that is thought to play an important role in both the traffic of
cholesterol to caveolae and modulating the activity of multiple signaling molecules at this site . -
pubmed
The region between
amino acids 71 and 80 controls incorporation of
caveolin-1 oligomers into detergent - resistant regions of the Golgi apparatus . -
pubmed
The binding ratios for
CTAB to
collagen ,
gelatin , DNA -
collagen and DNA -
gelatin mixtures respectively have also been determined . -
pubmed
The conformational structures of different biopolymers are observed to play significant role in macromolecular interactions between
protein and DNA in the presence of
CTAB . -
pubmed
The
protein - DNA -
CTAB interaction in mixture has been explained in terms of the deviation ( delta ) of ( gamma 2 m ) from ( gamma 2m)id in the presence of a surfactant in bulk . -
pubmed
The present retrieval and laboratory investigations have proven , that the morse taper junctions of the
MRP -
titanium stem are stable and resistant to relevant wear mechanisms . -
pubmed
The importance of the highly flexible , N - terminal region of
PrP has recently become more widely appreciated , particularly the biological activities associated with its metal ion - binding domain and its potential to form a poly(L -
proline )
II ( PPII ) helix . -
pubmed
Circular dichroism spectroscopy of an N - terminal peptide ,
PrP(37 - 53 ) , showed that the PPII helix is formed in aqueous buffer ; as it also contains an
Xaa - Pro - Gly consensus sequence , it may act as a substrate for the
collagen - modifying enzyme
prolyl 4 - hydroxylase . -
pubmed
Aberrant
CFTR - dependent
HCO3- transport in mutations associated with
cystic fibrosis . -
pubmed
Initially ,
Cl- conductance in the sweat duct was discovered to be impaired in
CF , a finding that has been extended to all
CFTR - expressing cells . -
pubmed
Subsequent cloning of the gene showed that
CFTR functions as a
cyclic - AMP - regulated Cl- channel ; and some CF - causing mutations inhibit
CFTR Cl- channel activity . -
pubmed
The identification of additional CF - causing mutants with normal
Cl- channel activity indicates , however , that other
CFTR - dependent processes contribute to the disease . -
pubmed
Indeed ,
CFTR regulates other transporters , including
Cl(-)-coupled
HCO3 - transport . -
pubmed
We have examined
Cl(-)-coupled
HCO3 - transport by
CFTR mutants that retain substantial or normal
Cl- channel activity . -
pubmed
Transcriptional repression by
Gal4 -
dHDAC1 was blocked by the
deacetylase inhibitor ,
FR901228 , and thus ,
deacetylase activity correlates with repression . -
pubmed
Brain perfusion was measured by
( 99m ) Tc - labeled bicisate (
ECD ) brain SPECT and the images were compared using Statistical Parametric Mapping ( SPM96 ) . -
pubmed
BACKGROUND : A recombinant humanized anti -
IgE mAb ,
omalizumab , forms complexes with free
IgE , blocking its interaction with mast cells and basophils ; as a consequence , it might be effective in the treatment of
asthma . -
pubmed
The
TTD - determined
DHT is a useful predictor of myocardial viability after an anterior
AMI . -
pubmed
The effect of
NCS-1 was specific for
GLUT4 and the
insulin - responsive aminopeptidase translocation as there was no effect on the trafficking of the
cation - independent mannose 6 - phosphate receptor or the
GLUT1 glucose transporter isoform . -
pubmed
The inhibitory action of
NCS-1 was independent of
calcium sequestration since neither treatment with
ionomycin nor
endothelin-1 , both of which elevated the intracellular
calcium concentration , restored
insulin - stimulated
GLUT4 translocation . -
pubmed
Endomorphin-1 (
EM-1 ) is a recently isolated endogenous peptide having potent analgesic activity and high affinity and selectivity for the
mu - opioid receptor . -
pubmed
Trypsin treatment reduced myocyte RF as shown by a decrease in the slope of the RF - SL relationship , and this was accompanied by a downward and leftward shift of the SL - pCa curve , indicative of sensitization of the myofilaments to
calcium . -
pubmed
In addition ,
trypsin digestion did not alter the relationship between SL and interfilament spacing ( assessed by cell width ) after
calcium activation . -
pubmed
The mechanisms of action of these new atypical antipsychotics can be classified into three general groups : a ) binding to D(2 ) together with non - dopaminergic receptors , b ) interaction with
dopamine receptor subtypes other than D(2 ) and c ) selective binding to non - dopaminergic systems , such as glutamatergic , sigma ,
neurotensin , and
cannabinoid . -
pubmed
All patients underwent complete
pulmonary function tests , which included spirometry , body plethysmography , and single - breath
carbon monoxide diffusion ( Dlco ) . -
pubmed
Adrenaline /
noradrenaline ratio in the adrenal glands of the
hedgehog during summer activity and hibernation . -
pubmed
Comparison of
ChIFN - alpha sequences , detected six
amino acids substitutions at positions 50 , 58 , 65 , 81 , 181 , and 183 . -
pubmed
In addition , both
SH - ChIFN - alpha and
WJ - ChIFN - alpha were expressed with the N - terminal 6 consecutive
histidine residues in a high - level prokaryotic expression system . -
pubmed
Localization of ectopic
corticotropin - producing
carcinoid tumor with use of
indium-111 pentetreotide scintigraphy . -
pubmed
OBJECTIVE : To report on the diagnosis of ectopic
corticotropin (
adrenocorticotropic hormone [
ACTH])-producing
bronchial carcinoid tumor by
indium-111 pentetreotide (
octreotide scan ) scintigraphy . -
pubmed
ACTH values returned to normal after resection of the lesion , and
octreotide scans confirmed the completeness of surgical resection . -
pubmed
Recent studies reveal that
statins stimulate the bone formation , presumably through increasing the production of
BMP-2 , an important differentiation factor of osteoblast . -
pubmed
Alkaline phosphatase (
ALP ) activity was maintained in the groups cryopreserved with
Me ( 2 ) SO and
glycerol . -
pubmed
In the present study , we have demonstrated SOCE - dependent sarcoplasmic reticulum (
SR )
Ca2 + loading ( load(SR ) ) that was not altered by inhibition of L - type
Ca2 + channels , reverse mode
Na+/
Ca2 + exchange (
NCX ) , or nonselective cation channels . -
pubmed
In contrast , lowering the extracellular [
Ca2 + ] to 0 mM or adding either 0.5 mM
Zn2 + or the putative store - operated channel ( SOC ) inhibitor
SKF-96365 ( 100 microM ) inhibited load(SR ) at
rest . -
pubmed
Interestingly , inhibition of forward mode
NCX with 30 microM
KB - R7943 stimulated SOCE significantly and resulted in enhanced load(SR ) . -
pubmed
In addition , manipulation of the extracellular and intracellular
Na + concentrations further demonstrated the modulatory role of
NCX in SOCE - mediated
SR Ca2 + loading . -
pubmed
Although there is little knowledge of SOCE in cardiomyocytes , the present results suggest that this mechanism , together with
NCX , may play an important role in SR
Ca2 + homeostasis . -
pubmed
These findings may be of particular importance during open heart surgery in neonates , in which uncontrolled SOCE could lead to
SR Ca2 + overload and arrhythmogenesis . -
pubmed
The macroparameter K(in)app is a composite of several kinetic terms defining the distribution volume of
FDOPA in brain ( V(e)D ) and the relative activity of
DOPA decarboxylase (
k3D ) . -
pubmed
The significant worsening of
CPT results did not correlate with changes in
FDOPA utilization . -
pubmed
CNV was induced using
krypton laser photocoagulation of the fundus , and the
CNV lesions were subsequently treated with PDT using light doses of 32 , 64 , and 83 s. Enucleated eyes were analyzed with light and transmission electron microscopies , and measurements of CNV size were done on histologic sections and on
isolectin B4 - stained choroidal flat mounts . -
pubmed
Hsp70 proteins assist refolding of polypeptides in an
ATP dependent manner . -
pubmed
Ultrasound contrast agent (
SonoVue ) was administered in continuous mode using an infusion pump (
BR - INF 100 , Bracco Research ) . -
pubmed
PURPOSE : :
Bevacizumab is a monoclonal antibody to
vascular endothelial growth factor (
VEGF ) which has been successfully used for the treatment of age - related
macular degeneration with
choroidal neovascularization . -
pubmed
These experiments tested the hypothesis that
vitamin E administration attenuates
nuclear factor kappaB (
NF - kappaB ) ,
IL-6 ,
IL-1beta and
tumour necrosis factor alpha (
TNFalpha ) responses in skeletal and cardiac muscle to an inflammatory challenge induced by systemic LPS . -
pubmed
Lipopolysaccharide - induced levels of cardiac
IL-1beta mRNA and protein and skeletal
IL-1beta mRNA were lower with
vitamin E than placebo . -
pubmed
Lipopolysaccharide - induced
NF - kappaB activation and increases in total
oxidized proteins were attenuated with
vitamin E compared with placebo in both tissues . -
pubmed
Vitamin E decreased LPS - induced increases in plasma
IL-1beta but not
IL-6 compared with placebo . -
pubmed
The major results provide the first in vivo evidence that short - term
vitamin E administration reduces
IL-6 and
IL-1beta responses to LPS in skeletal and cardiac muscle and prevents LPS - induced increases in
NF - kappaB activation and total oxidized proteins . -
pubmed
Although
TGA and
TAG were compositionally similar , we found a great variation in use of
TGA but a very limited range of use of
TAG . -
pubmed
The frequency of use of
TGA in the gene sequences generally increased with the
GC content of the chromosome , while the frequency of use of
TAG , like that of TAA , was inversely proportional to the
GC content of the chromosome . -
pubmed
The patterns of use of TAA ,
TGA and
TAG as real stop codons were less biased and less influenced by the
GC content of the chromosome . -
pubmed
The bias toward
TGA but against
TAG as a
PSC could not be explained either by the preferential usage of specific codons or by the
GC contents of individual chromosomes . -
pubmed
Peroxisome proliferator - activated receptor - gamma (
PPAR - gamma ) agonists (
thiazolidinediones ) have anti - inflammatory effects and improve endothelium function . -
pubmed
Pioglitazone given acutely after transient
brain ischemia / reperfusion reduced lesion size and the number of
Iba1 - expressing microglia in the ischemic striatum at three days . -
pubmed
However , analysis at 6 weeks after MCAo / reperfusion no longer yielded an effect of
pioglitazone on either lesion size or
Iba1 + cell counts . -
pubmed
Regarding functional longer - term outcome , we also did not detect a beneficial effect of
pioglitazone on motor function measured either on the
pole test or the wire hanging test or on learning and memory in the Morris water maze . -
pubmed
Pharmacological preconditioning of mesenchymal stem cells with
trimetazidine (
1 -[ 2,3,4 - trimethoxybenzyl ] piperazine ) protects hypoxic cells against oxidative stress and enhances recovery of myocardial function in infarcted heart through
Bcl-2 expression . -
pubmed
The preconditioned cells showed a significant protection against
H ( 2 ) O ( 2 )-induced loss of cellular viability , membrane damage , and
oxygen metabolism accompanied by a significant increase in
HIF-1alpha ,
survivin , phosphorylated
Akt (
pAkt ) , and
Bcl-2 protein levels and
Bcl-2 gene expression . -
pubmed
A significant increase in the recovery of myocardial function and up - regulation of
pAkt and
Bcl-2 levels were observed in hearts transplanted with
TMZ - preconditioned cells . -
pubmed
Therefore , we tested effects of oligomeric
Abeta on membrane fluidity of whole living cells , the impact of exogenous and cellular
Abeta on the processing of
APP and the role of GM-1
ganglioside . -
pubmed
We present evidence that oligoAbeta((1 - 40 ) ) stimulates the amyloidogenic processing of
APP by reducing membrane fluidity and complexing with GM-1
ganglioside . -
pubmed
Stability and selectivity of a
cholesterol - coated
C18 stationary phase . -
pubmed
Cholesterol loading onto a typical
C18 stationary phase is examined . -
pubmed
Nitric oxide stimulates
myoglobin gene and protein expression in vascular smooth muscle . -
pubmed
Mb (
myoglobin ) is a haemoprotein present in cardiac , skeletal and smooth muscle and is primarily responsible for the storage and ' facilitated transfer ' of molecular
oxygen from the cell membrane to mitochondria . -
pubmed
Overall , exposure of VSMCs ( either in cell culture or intact vessels ) to pathological *
NO promotes an up - regulation of the Mb gene and protein , suggesting a feedback relationship between *
NO and
Mb that regulates the concentration of the potent cell signalling molecule in the vessel wall , similar to the role
haemoglobin plays in the vessel lumen . -
pubmed
Cocaine self - administration alters the expression of chromatin - remodelling proteins ; modulation by
histone deacetylase inhibition . -
pubmed
Cocaine self - administration was accompanied by an increased synthesis of
Mecp2 ,
HDAC2 and
HDAC11 and by a decreased nuclear localization of
HDAC5 and of the
phospho - form of
HDAC5 , suggesting a nuclear export of this protein in response to the drug . -
pubmed
Among the genes we examined , treatment with
trichostatin A before each
cocaine self - administration session was found to mostly affect
Mecp2 and
HDAC11 expression . -
pubmed
A correlation was found between the modification of
Mecp2 and
MEF2C gene expression and the reinforcing property of
cocaine . -
pubmed
We found that
insulin and
glucose promoted
cancer cell proliferation and contributed to chemoresistance . -
pubmed
Both drugs affected signalling in the
protein kinases B (
AKT)/
mammalian target of rapamycin pathway ;
metformin activated
adenosine monophosphate ( AMP )- activated protein kinase whereas
rosiglitazone increased chromosome ten level . -
pubmed
Although high
insulin and
glucose concentrations promoted chemoresistance , the combination of
metformin or
rosiglitazone with
gemcitabine or
doxorubicin , resulted in an additional decrease in live
cancer cells and increase in apoptosis . -
pubmed
A membrane transport protein (
SEC61 ) was also characterised and this demonstrated the utility of the
clam sequence data as a resource for examining cold adapted
amino acid substitutions . -
pubmed
In conclusion , reduced expression of
NPY and increased expression of
NOS in hippocampus may make significant contributions to
CUMS - induced
depression . -
pubmed
UC - MSCs labeled with
carboxyfluorescein diacetate succinimidyl ester (
CFSE ) were found in the lungs and kidneys 1 week
post infusion . -
pubmed
Silver -
silver chloride electrodes placed at Oz ,
O1 and O2 were referred to Fz . -
pubmed
Axotomy - induced changes to the neuronal cytoskeleton and supporting astrocytes during the early stages after transection are delineated by examining the sequence of neurofilament subunit , microtubule (
TUB ) ,
microtubule associated protein (
MAP ) ,
glial fibrillary acidic protein (
GFAP ) and terminal deoxynucleotidyl transferase
biotin -
dUTP nick end labelling ( TUNEL ) modification . -
pubmed
Recent discoveries have led to an understanding of the biological role of
KIT and
platelet - derived growth factor receptor - alpha in
GIST and the development of the
tyrosine kinase inhibitor
imatinib mesylate (
Gleevec , formerly
STI-571 ) , one of the most exciting examples of targeted therapy to date . -
pubmed
Instability and aggregation problems were circumvented by expression of viral proteins fused to a carrier protein (
thioredoxin A ;
TrxA ) , purification via inclusion body formation ,
urea solubilization ,
His tag affinity chromatography and direct covalent coupling to microspheres without removal of the elution buffer . -
pubmed
The tetragonal prismatic SBU in 2 is connected to eight neighboring SBUs to form a 3 - D
MOF of
bcu net topology . -
pubmed
SynchFastSlow , a metric that is used in clinical depth - of - anesthesia monitors , showed a robust correlation with the loss of consciousness at the induction of
propofol GA , but this could be largely explained by power spectral changes without considering cross - frequency coupling . -
pubmed
The results suggest that the antidepressant - like activity of
DSS is probably mediated by the modulation of central
monoamine neurotransmitter systems and the reduction of oxidative stress . -
pubmed
Motif analysis predicted direct repeat with a spacer of one
nucleotide as the most prevalent
RXRalpha binding site . -
pubmed
Biological functional analysis of
RXRalpha - dependent genes revealed that
hepatic RXRalpha deficiency mainly resulted in up - regulation of
steroid and
cholesterol biosynthesis - related genes and down - regulation of translation- as well as anti - apoptosis - related genes . -
pubmed
Mitochondrial swelling has been observed following mitochondrial
calcium overload , and studies report that chelation of cytosolic
calcium induces extensive mitochondrial division at
ER contact sites . -
pubmed
Mitochondrial fission following
ER stress was abrogated with exposure of cells to the
AQP inhibitor
mercuric chloride , suggesting the involvement of
AQP ( s ) especially
AQP8 and
AQP9 known to be present in the mitochondrial membrane , in mitochondrial fission . -
pubmed
In participants in the highest vs the lowest quintile of nondairy dietary
calcium the multivariate relative risk of
kidney stones was 0.71 ( 95 % CI 0.56 - 0.92 , p for trend 0.007 ) in HPFS , 0.82 ( 95 % CI 0.69 - 0.98 , p trend 0.08 ) in
NHS I and 0.74 ( 95 % CI 0.63 - 0.87 , p trend 0.002 ) in NHS II . -
pubmed
When comparing the highest to the lowest quintile of dairy
calcium , the multivariate relative risk was 0.77 ( 95 % CI 0.63 - 0.95 , p trend 0.01 ) for HPFS , 0.83 ( 95 % CI 0.69 - 0.99 , p trend 0.05 ) for
NHS I and 0.76 ( 95 % CI 0.65 - 0.88 , p trend 0.001 ) for
NHS II . -
pubmed
We concluded that the oncocytotoxic effect of
dopamine was related to the induced polymerization of cytosol
actin . -
pubmed
MATERIAL AND METHODS : Composite resin ( AeliteFlo , Bisco , Schaumburg , IL , USA ) was inserted into a Class
II cavity prepared in the
Teflon blocks and was cured with a LED light curing unit ( Dr 's Light , GoodDoctors Co. , Seoul , Korea ) using various light curing modes for 20 s. Polymerization temperature was measured with an infrared thermographic camera ( Thermovision 900 SW / TE , Agema Infra - red Systems AB , Danderyd , Sweden ) for 40 s at measurement
spots adjacent to the cavity wall and in the middle of the cavity from the surface to a 4 mm depth . -
pubmed
On the other hand ,
hydralazine , with similar blood pressure lowering to
RD , did not significantly suppress the onset of
stroke and
brain injury in SHRSP . -
pubmed
Opiate use ( 7.6 % vs. 33 % ) , imaging (
6.3 % vs. 20.6 % ) , use of nonsteroidal anti - inflammatory drugs /
serotonin agonists at discharge ( 72.7 % vs. 22.2 % ) , and admission rates to hospital ( 22 % vs. 0 % ) were significantly different . -
pubmed
The present study was aimed to assess the potential to use the
Chk1 and
Chk2 inhibitor ,
AZD7762 , with other anticancer agents in chemotherapy to treat
ovarian clear cell carcinoma . -
pubmed
We treated the cells with
AZD7762 and anticancer agents , then assessed cell viability , cell cycle distribution , apoptosis , and the expression of protein in apoptotic pathways and molecules downstream of the
Chk signaling pathways . -
pubmed
AZD7762 effectively suppressed the
Chk signaling pathways activated by
cisplatin , dramatically enhanced expression of phosphorylated
H2A.X ,
cleaved caspase 9 and
PARP , decreased the proportion of cells in the gap 0/ gap 1 phase and the synthesis - phase fraction , and increased apoptotic cells . -
pubmed
OBJECTIVES : To determine whether a steerable catheter with electrogram guidance ( CS - assist group ) could facilitate access to the coronary
sinus (
CS ) during cardiac resynchronization therapy (
CRT ) implantation . -
pubmed
The
atonal genes are an evolutionarily conserved group of genes encoding regulatory
basic helix - loop - helix ( bHLH ) transcription factors . -
pubmed
Bilirubin ,
prothrombin time ,
creatinine ,
potassium at admission , and a spontaneous change in
bilirubin levels from day 0 to day 7 ( SCBL ) were incorporated into a model for
AH to grade the severity in an Asian patient cohort ( MAGIC ) . -
pubmed
Cardiac glycoside - mediated effects on NMD are dependent on binding and inhibiting the
sodium - potassium ATPase on the plasma membrane and subsequent elevation of intracellular
calcium levels . -
pubmed
Linear regression modeling was used to evaluate the association of serum
leptin level with
glomerular filtration rate calculated using the plasma
iohexol disappearance curve , demographics ,
body mass index (
BMI ) , and cardiovascular risk factors , including inflammatory
cytokines ,
insulin resistance , and serum lipid levels . -
pubmed
Leptin level also correlated positively with serum
triglycerides and
insulin resistance ( P & lt ; .001 ) and negatively with serum
high - density lipoprotein cholesterol ( P = .002 ) . -
pubmed
Leptin level was not associated with
glomerular filtration rate calculated using the plasma
iohexol disappearance curve or inflammatory
cytokines . -
pubmed
In multivariate analysis , BMI , age , female sex , and serum
triglyceride levels were significantly associated with serum
leptin level . -
pubmed
Opposite
face sensitivity of
CeO2 in hydrogenation and oxidation catalysis . -
pubmed
OBJECTIVES : To assess if coronary artery
calcium (
CAC ) scans influence treatment patterns as reflected by subsequent rates of cardiac imaging and therapeutic interventions , and their effect on ischemic events downstream . -
pubmed
Discrete decreased activity in the lower thoracic spine on
FDG PET /
CT : another respiration - related artifact . -
pubmed
The aim of this study was to develop and evaluate the effects of chitosan inserts for sustained release of the
angiotensin - converting enzyme 2 (
ACE2 ) activator ,
diminazene aceturate (
DIZE ) , in experimental
glaucoma . -
pubmed
Monolayer
DIZE loaded inserts ( D+I ) were prepared and characterized through swelling , attenuated total reflectance Fourier transformed infrared spectroscopy (
ATR - FTIR ) , differential scanning calorimetry ( DSC ) and in vitro drug release . -
pubmed
Biochemical investigations including
GAA ,
creatine and
GAMT enzyme activity measurements are essential to confirm the diagnosis of
GAMT deficiency . -
pubmed
The present study aims to assess the removal of
3 - amino-5 - methylisoxazole (
AMI ) , a recalcitrant by - product resulting from the biological breakdown of some pharmaceuticals , applying a solar photo - Fenton process assisted by
ferrioxalate complexes ( SPFF ) (
Fe3 +/ H2O2 /
oxalic acid /
UVA - Vis ) and classical solar photo - Fenton process (
SPF ) (
Fe2 +/ H2O2 / UVA - Vis ) . -
pubmed
The oxidation power of the classical
SPF was assessed at different pH values ( 2.8 - 4.0 ) using 2.0 mg
Fe2 + per liter . -
pubmed
Furthermore , the effect of
AMI concentration ( 2 - 20 mg L-1 ) , presence of inorganic ions (
Cl- ,
SO42 - ,
NO3 - ,
HCO3 - ,
NH4 + ) , and radical scavengers (
sodium azide and
D - mannitol ) on the
SPF method at pH 3.5 was also assessed . -
pubmed
Oxalic and oxamic acids were identified as the main low - molecular - weight
carboxylic acids detected during the pilot - scale
SPF reaction . -
pubmed
METHODS : Concentration - response curves for
phenylephrine ( PE ) were obtained in strips of corpus cavernosum , in absence or after incubation with
tiron ,
tempol or
polyethylene glycol (
PEG)-
catalase combined or not with
tempol . -
pubmed
The combination of
PEG -
catalase and
tempol did not alter the contractile response to
PE in both groups . -
pubmed
HHcy increased
O2 (- ) generation and
SOD activity , whereas
H2O2 concentration was reduced . -
pubmed
In this paper , removal of
copper ions from aqueous solution using novel xanthan gum (
XG ) hydrogel , xanthan gum -
graft-2 - acrylamido-2 - methyl-1 - propane sulfonic acid (
XG - g - P(
AMPS ) ) hydrogel and xanthan gum -
graft-2 - acrylamido-2 - methyl-1 - propane sulfonic acid /
montmorillonite ( XG - g -
P ( AMPS )/ MMT ) hydrogel composite were studied . -
pubmed
Adsorption process was found to follow Langmuir isotherm model with maximum adsorption capacity of 24.57 , 39.06 and 29.49 mg / g for the
XG ,
XG - g - P(AMPS ) and
XG - g -
P ( AMPS )/ MMT , respectively . -
pubmed
5 '- deoxyadenosine (
dAH ) ,
S -( 5 '- Adenosyl )- l - cysteine (
AdoCy ) and
S -( 5 '- Adenosyl )- l - homocysteine (
AdoHcy ) were competitive inhibitors of
MtbBS with the following inactivation parameters : Ki = 24.2 muM , IC50 = 267.4 muM ; Ki = 0.84 muM , IC50 = 9.28 muM ; and Ki = 0.592 muM , IC50 = 6.54 muM for
dAH ,
AdoCy and
AdoHcy respectively . -
pubmed
Mechanistically ,
Tets depletion or deficiency increases
Dnmt3b and decreases
5hmC levels , resulting in elevated methylation levels at sub - telomeres . -
pubmed
During surgery , samples of
CSF were collected from the lumbar and ventricular compartments and assayed for
chloride ,
glucose and
total protein . -
pubmed
Hearts with
MR had a 53 % decrease in
desmin ( P & lt ; .0001 ) and a 2.6 - fold increase in
desmin aggregates ( P & lt ; .0001 ) versus normal , along with substantial , intense perinuclear staining of
alpha , beta - unsaturated aldehyde 4 - hydroxynonenal in areas of mitochondrial breakdown and clustering . -
pubmed
These findings suggest that
mGlu2 is at least in part necessary for the
5 - HT2A receptor - dependent
psychosis - like behavioral effects induced by
LSD - like drugs . -
pubmed
CMG pre - treatment also restored mitochondrial
complex I and IV activities inhibited by
ROT . -
pubmed
CMG induced anti - apoptotic effects was substantiated by decreased phosporylation of
JNK3 and
c - jun , which in turn decreased the cleavage of
pro - caspase 3 . -
pubmed
Q - PCR analysis of redox genes showed up - regulation of
NOS2 and down - regulation of
NQO1 upon
ROT exposure and this was attenuated by
CMG pre - treatment . -
pubmed
Herein , we report a new bottom - up synthetic method utilizing
ammonium fluoride (
NH4F ) for the preparation of
anhydrous MFx (
CuF2 ,
FeF3 , and
CoF2)/mesoporous
carbon (
MSU - F - C ) nanocomposites , whereby a series of metal precursor nanoparticles preconfined in mesoporous carbon were readily converted to anhydrous MFx through simple heat treatment with NH4F under solventless conditions . -
pubmed
Here , systematic density functional theory calculations demonstrate that
metal sulfide fullerenes Sc2S@
Cn have similar cage geometries from C70 to C84 and form a close - knit family of structures related by Endo - Kroto insertion / extrusion of
C2 units and Stone - Wales isomerization transformations . -
pubmed
SMF of either orientation reduced
systolic blood pressure variability in very low frequency domain while downward - oriented
SMF also reduced low - frequency and increased high frequency domains . -
pubmed
Emergence of RASs relevant only to the
polymerase non -
nucleoside inhibitors ( NNI ) was detected , and these lay within
CD8 + T - cell epitopes . -
pubmed
It provides an alternative
MRS tool for in vivo study of metabolic coupling relationship between aerobic and anaerobic
glucose metabolisms in brain under physiopathological states . -
pubmed
Both
Pten and
Nras are downstream mediators of receptor
tyrosine kinase activation that plays important roles in controlling cell survival and proliferation . -
pubmed
At the molecular level , lipid droplet formation was primarily contributed by upregulated expression of genes responsible for lipogenesis and
fatty acid sequestration , such as
Srebpf1 ,
Acc ,
Pparg and its downstream targets . -
pubmed
From the in silico prediction of
PRAME gene structure , we identified the putative
myeloid zinc finger 1 (
MZF1 ) binding sites , which overlap with a
CpG - rich region located in the first intron . -
pubmed
Upon treatment with a DNA methylation inhibitor ,
5 - aza-2 '- deoxycitidine (
5 - azaC ) , together with ectopic expression of
MZF1 ,
PRAME expression was significantly enhanced at both the protein and mRNA levels . -
pubmed
More pronounced
MZF1 binding to the
PRAME DNA was observed in the presence of
5 - azaC. -
pubmed
Molecular mechanism of
PD-1 /
PD - L1 blockade via
anti - PD - L1 antibodies
atezolizumab and
durvalumab . -
pubmed
In 2016 and 2017 , monoclonal antibodies targeting
PD - L1 , including
atezolizumab ,
durvalumab , and
avelumab , were approved by the FDA for the treatment of multiple advanced
cancers . -
pubmed
Recently , the crystal structures of
PD - L1 in complex with
BMS-936559 and
avelumab have been determined , revealing details of the antigen - antibody interactions . -
pubmed
However , it is still unknown how
atezolizumab and durvalumab specifically recognize
PD - L1 , although this is important for investigating novel binding sites on
PD - L1 targeted by other therapeutic antibodies for the design and improvement of
anti - PD - L1 agents . -
pubmed
Here , we report the crystal structures of
PD - L1 in complex with
atezolizumab and
durvalumab to elucidate the precise epitopes involved and the structural basis for
PD-1 /
PD - L1 blockade by these antibodies . -
pubmed
Thioredoxin-1 maintains mechanistic target of
rapamycin (
mTOR ) function during oxidative stress in cardiomyocytes . -
pubmed
Thioredoxin 1 (
Trx1 ) is a 12 - kDa
oxidoreductase that catalyzes
thiol -
disulfide exchange reactions to reduce proteins with
disulfide bonds . -
pubmed
Mechanistic target of
rapamycin (
mTOR ) is a
serine /
threonine kinase that regulates cell growth , metabolism , and survival . -
pubmed
In response to
H2O2 treatment in cardiomyocytes ,
mTOR exhibited a high molecular weight shift in non - reducing
SDS - PAGE in a
2 - mercaptoethanol - sensitive manner , suggesting that
mTOR is oxidized and forms
disulfide bonds with itself or other proteins . -
pubmed
Immune complex kinase assays disclosed that
H2O2 treatment diminished
mTOR kinase activity , indicating that
mTOR is inhibited by oxidation . -
pubmed
Of note ,
Trx1 overexpression attenuated both
H2O2 - mediated
mTOR oxidation and inhibition , whereas
Trx1 knockdown increased
mTOR oxidation and inhibition . -
pubmed
Moreover ,
Trx1 normalized
H2O2 - induced down - regulation of metabolic genes and stimulation of cell
death , and an
mTOR inhibitor abolished
Trx1 - mediated rescue of gene expression . -
pubmed
H2O2 - induced oxidation and inhibition of
mTOR were attenuated when
Cys-1483 of
mTOR was mutated to
phenylalanine . -
pubmed
These results suggest that
Trx1 protects cardiomyocytes against stress by reducing
mTOR at
Cys-1483 , thereby preserving the activity of
mTOR and inhibiting cell
death . -
pubmed
The temporal effect of
transforming growth factor beta 3 (
TGFbeta3 ) and
fibroblast growth factor 2 (
FGF2 ) on the derivation of proliferative chondrocytes from MSCs in three - dimensional
agarose was investigated by manipulating the duration of
TGFbeta3 and
FGF2 treatment . -
pubmed
Interleukin-33 - Activated Islet - Resident Innate Lymphoid Cells Promote
Insulin Secretion through Myeloid Cell
Retinoic Acid Production . -
pubmed
Here we show that
interleukin ( IL )- 33 was produced by islet mesenchymal cells and enhanced by a
diabetes milieu (
glucose ,
IL-1beta , and
palmitate ) . -
pubmed
IL-33 promoted beta cell function through islet - resident group 2 innate lymphoid cells ( ILC2s ) that elicited
retinoic acid ( RA)-producing capacities in macrophages and dendritic cells via the secretion of
IL-13 and
colony - stimulating factor 2 . -
pubmed
Disruption of
TFGbeta -
SMAD3 pathway by the nuclear receptor
SHP mediates the antifibrotic activities of
BAR704 , a novel highly selective
FXR ligand . -
pubmed
The
farnesoid - x - receptor (
FXR ) is a
bile acid sensor that counteracts HSCs transdifferentiation . -
pubmed
While targeting
FXR holds promise ,
6 - ethyl - CDCA known as
obeticholic acid , the first in class of
FXR ligands , causes side effects , partially because the lack of selectivity toward
GPBAR1 , a putative itching receptor . -
pubmed
Here , we describe the
3 - deoxy-6 - ethyl derivative of
CDCA ,
BAR704 , as a highly selective steroidal
FXR agonist . -
pubmed
MAIN RESULTS : In transactivation assay
BAR704 activated
FXR with and EC50 of 967 nM while exerted no agonistic activity on other receptors including
GPBAR1 . -
pubmed
CONCLUSIONS AND APPLICATIONS :
BAR704 is a selective
FXR agonist that reduces
liver fibrosis by interfering with the
TGFbeta -
SMAD3 pathway in HSCs . -
pubmed
Herein , we detected an SSR embedded in the promoter region of the
CYP82Y1 gene , which was found to be the first committed - step enzyme in the
noscapine biosynthesis pathway , using the MISA program . -
pubmed
METHODS : Following a legislative change that allowed EMTs to administer
naloxone in one rural state , we evaluated an
EMT training program by examining EMTs ' opioid
overdose knowledge and attitudes before and after the training . -
pubmed
The controlled pyrolysis of
ZIF-67 can lead to the formation of
Co nanoparticles encapsulated in
nitrogen - doped porous
carbon (
Co NC ) , which subsequently underwent galvanic replacement with
Na2PdCl4 to form
Co@
Pd core - shell nanoparticles embedded in
nitrogen - doped porous
carbon (
Co@
Pd NC ) . -
pubmed
The
Co@
Pd NC exhibited outperformance in ORR and
HER than commercial
Pd / C , as manifested by more positive onset potential and larger diffusion - limited current density in ORR tests , as well as a small overpotential to drive a current density of 10 mA cm-2 , and much lower Tafel slope in HER tests . -
pubmed
Estimation of pancreatic
R2 * for
iron overload assessment in the presence of fat : a comparison of different approaches . -
pubmed
Altitude training was first used to prepare for competition at altitude , and it still is
today ; however , more often now , elite athletes embark on a series of altitude training
camps to try to improve sea - level performance . -
pubmed
Furthermore , liquid chromatography - mass spectrometry ( LC -
MS ) analysis of
ethyl acetate extract identified 80 different compounds based on mass - to - charge ratio , database difference , resolution of mass spectrum and so on . -
pubmed
Herein , a rigid
squarate - based metal - organic framework (
MOF ) , having a perfect pore size ( 4.1 A x 4.3 A ) comparable with the kinetic diameter of
Xe ( 4.047 A ) as well as pore surface decorated with very polar
hydroxyl groups , is able to effectively discriminate
Xe atoms , affording a record - high
Xe /
Kr selectivity . -
pubmed
The
MOF exhibits the highest
Xe Henry coefficient ( 192.1 mmol / g / bar ) and
Xe /
Kr Henry selectivity ( 54.1 ) among all state - of - the - art adsorbents reported so far . -
pubmed
However ,
enzymes that target
diterpenoid compounds and show specific activity for side - chain
glucosyl groups are rare . -
pubmed
SPBGL1 displayed high specificity toward
sophorose , and activity toward
stevioside , but not
rebaudioside A. -
pubmed
SPBGL1 also operated at high substrate concentrations , such as in 50 % crude
steviol glycoside extract . -
pubmed
Glucose liberated from
stevioside was easy to quantify using the
glucose oxidase method , allowing the
stevioside content to be determined . -
pubmed
Simply linear regression analysis demonstrated a positive correlation between serum
preptin and gender ,
body mass index (
BMI ) , blood
urea nitrogen ,
creatinine ,
ACR , and a negative correlation between serum
preptin and
glomerular filtration rate ,
metformin ,
acarbose treatment . -
pubmed
A label - free immunosensor based on
PtPd NCs@
MoS2 nanoenzymes for
hepatitis B surface antigen detection . -
pubmed
We introduced new nanoenzymes to a label - free electrochemical immunosensor for
Hepatitis B surface antigen (
HBs Ag ) detection . -
pubmed
The prepared nanoenzymes exhibited enhanced
peroxidase - like activity than separate
MoS2 and
PtPd NCs . -
pubmed
PtPd NCs@
MoS2 were used for sensitive detection of HBs
Ag , which is ascribed to their superior
peroxidase activity , good conductivity and high specific surface area and synergistic amplification for current signals . -
pubmed
Glibenclamide ameliorates transplant - induced
arteriosclerosis and inhibits macrophage migration and
MCP-1 expression . -
pubmed
We used
hematoxylin and
eosin (
HE ) and Elastic Van Gieson ( EVG ) staining for histological assessment , and qRT - PCR and ELISA to measure mRNA and protein levels . -
pubmed
Morphologically ,
glibenclamide reduced inflammatory cell accumulation and
collagen deposition in the aortas . -
pubmed
Furthermore ,
glibenclamide inhibited macrophage expression and secretion of
inflammatory factors in vitro through suppressing activation of the
nuclear factor - kappaB (
NF - kappaB ) pathway and
MCP-1 production . -
pubmed
CONCLUSION :
Glibenclamide protected against aorta transplantation - induced
arteriosclerosis by reducing inflammatory factors in vivo and inhibited macrophage migration and
MCP-1 production in vitro . -
pubmed
Elevated serum
17 - hydroxyprogesterone (
17 - OHP ) and
CYP21 gene molecular analysis confirmed the diagnosis . -
pubmed
H2S mediates apoptosis in response to
inflammation through
PI3K /
Akt /
NFkappaB signaling pathway . -
pubmed
Moreover , exogenous
H2S rescued LPS - induced
cystathionine gamma - lyase (
CSE ) inhibition and
cystathionine beta - synthase (
CBS ) synthesis . -
pubmed
Interestingly , in cells undergoing
inflammation - induced apoptosis ,
H2S activated the
PI3K /
Akt and
NFkappaB signal pathways both tested concentrations . -
pubmed
CONCLUSIONS :
H2S regulates LPS - induced
inflammation and apoptosis by activating the
PI3K /
Akt /
NFkappaB signaling pathway . -
pubmed
FTIR showed that
O - H bond was destroyed after torrefaction for both
FRP and SP . -
pubmed
18F - FDG PET /
CT is limited to aggressive forms and PET imaging using
68Ga - somatostatin analogs appears suboptimal . -
pubmed
Using a set of compounds ( new derivatives , known products and
GABAA subtype receptor ligands from our library ) we identify the
amino acids at the alpha+/gamma- interface , which could be involved in the agonist or antagonist profile , using the ' Proximity Frequencies ' , namely the frequencies with which a ligand intercepts two or more binding - site
amino acids during the molecular dynamic simulation . -
pubmed
Severity and time course of these phenomena suggest that early institution of direct (
Trasylol ) or indirect (
heparins , cortocosteroids )
proteinase inhibition , and if necessary a replacement of the lost factors , should be considered . -
pubmed
[
C - erbB-2 oncogene amplification in
breast cancer in correlation to
steroid and
epidermal growth factor receptor ] . -
pubmed
Lipid peroxidation parameters , such as
malonic dialdehyde and antioxidant defense (
superoxide dismutase and
catalase activities ) were studied in healthy individuals , miners of different occupations , working at mines of different altitudes . -
pubmed
Loss of
thrombin - induced
Ca2 + mobilization in a subpopulation of platelets during storage . -
pubmed
The maximal concentration of [
Ca2 +] i after stimulation with 0.5 U of
thrombin / ml decreased from 544 + /- 58 nM ( mean + /- SEM , n = 6 ) on day 0 , to 276 + /- 9 nM on day 3 and to 203 + /- 23 nM on day 6 . -
pubmed
The study shows that during platelet storage a decrease in the rise in [
Ca2 +] i upon
thrombin stimulation occurs . -
pubmed
ADP ribosylation and
G protein regulation in the thyroid . -
pubmed
Group I received no antiplatelet agents ( control ) ; group
II received U-63,557A , a specific
thromboxane synthetase inhibitor ( TSI ) ; group III received
aspirin ; and group
IV received
aspirin and TSI . -
pubmed
The fact that platelets , PMN leukocytes , and
MN leukocytes concentrate
ascorbic acid suggests that
vitamin C has an important role in their physiological functions . -
pubmed
They also found that
MN leukocytes , in contrast , do not show any such relationship ; however ,
MN leukocytes maintain the highest levels of
ascorbic acid and play a very important function in immunocompetence . -
pubmed
We have found that with a limited number of subjects ,
ascorbic acid content of
MN and PMN leukocytes correlates positively with plasma
ascorbic acid , but there was no correlation between platelets and plasma
ascorbic acid ( unpublished results ) . -
pubmed
These effects of
PAF on
LDL and lipid metabolism could be related to perturbations in membrane structure characteristics , leading to a delay in
LDL delivery to lysosomes , and to modification of the activity of some key enzymes of lipid metabolism . -
pubmed
LDH isoenzymes were studied using electrophoresis in
polyacrylamide gel . -
pubmed
Thyrotropin- and
prostaglandin E2 - responsive
adenyl cyclase in thyroid plasma membranes . -
pubmed
[ Effect of
carbonic anhydrase inhibitor ,
benzanilamide , on activity of
carbonic anhidrase in the ciliary body and intraocular pressure of glaucomatous eyes ] . -
pubmed
Studies on the effect of
thyroxine on in vivo
insulin secretion as modified by hypophysectomy . -
pubmed
The finding that
alprazolam was effective in the treatment of
panic disorder is of interest as this diagnostic category is usually treated with tricyclic antidepressants or
MAO inhibitors . -
pubmed
Lithium chloride lacked a direct effect on
tyrosine hydroxylase (
TH ) ,
monoamine oxidase (
MAO ) , or
catechol - O - methyltransferase (
COMT ) in concentrations up to 2 mM. -
pubmed
[ Urinary
kallikrein excretion ,
renin -
angiotensin -
aldosterone system and urinary
electrolytes excretion in
hypertensive outpatients ] . -
pubmed
Treatment with both
oxygen and subcutaneous
sumatriptan reduced the
CGRP level to normal , while opiate administration did not alter the peptide levels . -
pubmed
Zinc is a cofactor for
alcohol dehydrogenase , the
ethanol metabolizing enzyme . -
pubmed
Adverse effects of
Plachitin particles for arterial chemoembolization were
epigastralgia ,
nausea ,
fever , and elevation of serum
aspartate aminotransferase (
AST ) and
alanine aminotransferase (
ALT ) levels . -
pubmed
Monoclonal antibodies (
MoAbs ) specific for
glycyrrhetic acid (
GA ) were prepared and characterized . -
pubmed
Obtained
MoAbs ( AGA-1 , AGA-3 , and AGA-6 ) reacted with
GA dose - dependently , but not with
glycyrrhizin (
GL ) ,
carbenoxolone , and
steroids . -
pubmed
5 - Lipoxygenase has been recognized to be an important enzyme that catalyzes the first step in
leukotriene production . -
pubmed
Among the synthesized phospholipids examined ,
1 - oleoyl-2 - docosahexaenoyl - sn - glycero-3 - phosphocholine (
1 - oleoyl-2 - DHA - PC ) was found to be the most potent inhibitor of
5 - lipoxygenase . -
pubmed
Lineweaver - Burk plot analysis showed that the inhibition of
5 - lipoxygenase by
1 - oleoyl-2 - DHA - PC was non - competitive . -
pubmed
The inhibition by this synthesized
phospholipid was very specific to
5 - lipoxygenase ; that is , it did not extend to
fatty acid cyclooxygenase ,
12 - lipoxygenase or
15 - lipoxygenase . -
pubmed
These results suggest that endogenously existing
DHA - containing phospholipids may affect
5 - lipoxygenase activity and thus control
leukotriene biosynthesis in vivo . -
pubmed
They were incubated for isolation in
Hanks ' balanced salt solution with 0.35 %
collagenase and Earle 's
solution with 1 mM
EDTA . -
pubmed
NG - nitro - D - arginine methyl ester (
D - NAME , a less active inhibitor of
NO synthase ) showed no effects in the above behavioural tasks . -
pubmed
Quantitative
DNase I footprint titration experiments reveal that ImPyPy - gamma - PyPyPy - beta - Dp binds all eight 5'-TG(A , T)(3)-3 ' target sites with only a 12 - fold difference in the equilibrium association constants between the strongest site , 5'-TGTTT-3 ' ( Ka = 2.1 x 10(8 ) M-1 ) , and the weakest site , 5'-TGAAT-3 ' ( Ka = 1.8 x 10(7 ) M-1 ) ( 10 mM
Tris . -
pubmed
INTERVENTIONS : Each family member completed a questionnaire and underwent expiratory flow measurement , skin prick tests for
allergies ,
methacholine tests and measurement of total serum
IgE level and blood eosinophil count . -
pubmed
RESULTS : The group of families with a member with
asthma had an increased prevalence of
atopy and
AHR , lower PC20
methacholine and higher serum
IgE levels in comparison with controls , even when the first identified family members with
asthma and their matched controls were not included . -
pubmed
There was a significant correlation between PC20
methacholine level and atopic index , between blood eosinophil count and serum
IgE level , and between atopic index and serum
IgE level , for all groups . -
pubmed
Single
nucleotide modulation of
uridine to
pseudouridine rearrangement in transfer RNA catalyzed by
pseudouridine synthase I. -
pubmed
coli
pseudouridine synthase I (
PSUI ) catalyzes the rearrangement of
uridine residues in positions 38 , 39 and 40 of
tRNA transcripts to
pseudouridine . -
pubmed
Receptor expression was observed in cells that had earlier been shown to express
cGMP - dependent kinase , but not the
cystic fibrosis transmembrane regulator , a known downstream target of
cGMP / G - kinase , which suggests that
GCC/
cGMP could regulate additional cellular signal transduction machinery . -
pubmed
In addition to
histamine , MC contain many other angiogenic factors and a variety of
cytokines ,
growth factors , and proteolytic enzymes implicated in tissue remodeling , and normal as well as
tumor - associated neoangiogenesis . -
pubmed
Chronic administration of
PTU or
PTU plus T4 inhibited the
3 beta - hydroxysteroid dehydrogenase ,
21 beta - hydroxylase , and
11 beta - hydroxylase activities . -
pubmed
In cultured cells transfected with the
human TSH receptor ,
hCG increases generation of
cAMP . -
pubmed
Molecular variants of
hCG with increased thyrotropic potency include basic molecules with reduced
sialic acid content , truncated molecules lacking the C - terminal tail , or molecules in which the 47 - 48 peptide bond in the beta - subunit loop is nicked . -
pubmed
In normal pregnancy , when
hCG levels are highest at 10 to 12 weeks gestation , there is suppression of
serum TSH levels , presumably due to slight increases in free
thyroxine ( T4 ) concentration . -
pubmed
Furthermore , an increased toxicity was observed when
Al was concurrently administered with CA ,
SA , VA ,
PCA , and HY . -
pubmed
Inversely , significant cytoprotective effects were noted with
EDTA , HA , CFA , and
PA . -
pubmed
The [ rho(0 ) ] mutants and
antimycin - treated cells differ from the wild type in PPM composition and
invertase ,
acid phosphatase , and
glucoamylase activities . -
pubmed
The reduced flocculation of cells grown in the presence of
antimycin , under anaerobiosis , or carrying a [ rho(0 ) ] mutation may be the consequence of alterations of PPM structures which are the ligands of
lectins , both involved in this cell - cell recognition phenomenon . -
pubmed
dose - dependently enhanced ( 9/26 neurons ) or suppressed ( 8/26 ) activity , and the
5 - HT1A antagonist
( + ) WAY-100135 often attenuated these responses . -
pubmed
CONCLUSION : The lack of correlation between total
GST and
protease activity for most of the enzymes investigated and
alanyl AP levels in perfusate samples could be related to differences in cellular localisation , suggesting that assays of
alanyl AP may give complimentary biochemical information relating to kidney tissue damage . -
pubmed
Here we report more details on one of the genes identified , a
hemN - like gene ( now called
hemN ( 1 ) ) whose product exhibits significant similarity to
oxygen - independent
coproporphyrinogen III dehydrogenases involved in
heme biosynthesis in facultatively anaerobic bacteria . -
pubmed
The
hemN ( 2 ) gene maps outside of the symbiotic gene region ; it is located 1.5 kb upstream of
nirK , the gene for a
Cu - containing
nitrite reductase . -
pubmed
The two deduced
HemN proteins are similar in size ( 445 and 450
amino acids for
HemN(1 ) and
HemN(2 ) , respectively ) and share 53 % identical ( 68 % similar )
amino acids . -
pubmed
Moreover , these mutants lacked several
c - type cytochromes which are normally detectable by
heme staining of proteins from anaerobically grown wild - type cells . -
pubmed
The results are discussed in terms of energization of
SE transporters for retrieval of leaking
sucrose ,
K + and
amino acids , as one of the unknown roles of
ATP found in SEs . -
pubmed
The function of the
PPase could be related to phloem
sucrose metabolism in support of
ATP - requiring processes . -
pubmed
Rapid degradation of
auxin /
indoleacetic acid proteins requires conserved
amino acids of domain
II and is proteasome dependent . -
pubmed
Auxin rapidly induces auxin /
indoleacetic acid (
Aux /
IAA ) transcription . -
pubmed
The C - terminal 109
amino acids did not affect
LUC half - life . -
pubmed
New data reveal that
insulin may regulate this process in part by promoting membrane trafficking of intracellular
fatty acid transporters
FATP1 and
FATP4 to the plasma membrane . -
pubmed
Exonic splicing enhancer - dependent splicing of the
gonadotropin - releasing hormone premessenger ribonucleic acid is mediated by
tra2alpha , a 40 - kilodalton
serine /
arginine - rich protein . -
pubmed
In an earlier study , we found that excision of the first intron (
intron A ) from the
rat GnRH primary transcript is attenuated in non -
GnRH - producing cells . -
pubmed
In the presence of an ESE in exon 4 ( ESE4 ) , an addition of
GT1 NE further increased the excision rate of
intron A , whereas the addition of
KK1 ( a non -
GnRH - producing cell ) NE decreased it . -
pubmed
To define the
GnRH neuron - specific splicing activity ,
GT1 NE was fractionated by ultracentrifugation and
ammonium sulfate precipitation . -
pubmed
A 50 - 90 %
ammonium sulfate pellet ( ASP50 - 90 ) fraction was further precipitated with 20 mM
MgCl ( 2 ) to isolate a
serine /
arginine - rich (
SR ) protein fraction . -
pubmed
Among the
ASP fractions ,
ASP40 - 50 significantly increased the excision rate of
intron A in the presence of HeLa NE or
SR protein - rich fraction . -
pubmed
These results imply that
Tra2alpha and a cofactor protein in the
ASP40 - 50 fraction are involved in mediating the
GnRH neuron - specific excision of intron A from the
GnRH primary transcript . -
pubmed
OBJECTIVES : To study the relationship between serum levels of
hyaluronic acid ( HA ) ,
type III procollagen (
PCIII ) ,
laminin (
LN ) ,
type IV collagen (
IV - C ) and
hepatic fibrosis and to determine their value in clinical practice . -
pubmed
FINDINGS : After a mean follow - up of 5.1 years ,
fluvastatin lowered
LDL cholesterol concentrations by 32 % . -
pubmed
Sodium dodecyl sulfate at pH 6.0 -
6.3 cleaves the DNA of isolated gelable nuclei from the residual protein of these nuclei over a period of 2 to 3 hours . -
pubmed
BACKGROUND :
Tacrolimus is an immunosuppressive drug that is a substrate of
cytochrome P450 3A (
CYP3A ) enzymes and
P - glycoprotein (
P - gp ) . -
pubmed
Corticosteroids induce both
CYP3A and
P - gp activity . -
pubmed
After treated with
genistein ,
BMD and the serum level of
ALP ,
ACP ,
osteocalcin increased significantly , while the serum level of
IL-1beta and
TNF - alpha decreased . -
pubmed
The protein has a fold similar to that of
disulfide reductases and consists of the
FAD - binding ,
NAD - binding , and C - terminal domains . -
pubmed
Compared to homologous
flavoenzymes , the
reductase component of
biphenyl dioxygenase (
BphA4 ) and
apoptosis - inducing factor ,
Pdr lacks one of the
arginine residues that compensates partially for the negative charge on the
pyrophosphate of
FAD . -
pubmed
The aromatic side - chain of the " gatekeeper "
Tyr159 is in the " out " conformation and leaves the
nicotinamide - binding site of
Pdr completely open . -
pubmed
A structural comparison of
Pdr with homologous
flavoproteins indicates that an open and accessible
nicotinamide - binding site , the presence of an acidic residue in the middle part of the
NAD - binding channel that binds the
nicotinamide ribose , and multiple positively charged
arginine residues surrounding the entrance of the
NAD - binding channel are the special structural elements that assist tighter and more specific binding of the oxidized
pyridine nucleotide by the
BphA4 - like
flavoproteins . -
pubmed
Here , it is reported that deletion of
ppoB (
DeltappoB ) reduced production of the
oleic - acid - derived
oxylipin psiBbeta and increased the ratio of asexual to sexual spore development . -
pubmed
Generation of the triple mutant Delta
ppoA Delta
ppoB Delta
ppoC resulted in a
strain deficient in producing
oleic - and
linoleic - acid - derived
8 '- hydroxy psi factor and caused increased and mis - scheduled activation of sexual development . -
pubmed
The purpose of this study was to investigate the localization and distribution of ferrofluid microsphere of
human serum albumin methotrexate ( FM -
HSA -
MTX ) carriers in the brain and to explore the magnetic targeting chemotherapy for
malignant brain tumor . -
pubmed
Synthesized FM -
HSA -
MTX carriers (
MTX 25 mg / kg ) were injected into the systemic circulation via the caudal vein ( magnetic targeting group , n = 30 ) . -
pubmed
The non - magnetic targeting group ( n = 30 ) was administered with FM -
HSA -
MTX without external magnetic field , meanwhile the control group ( n = 30 ) was treated with
MTX and a magnetic field . -
pubmed
We measured urinary
iodine ( UI ) ,
thyroid - stimulating hormone (
TSH ) , and total
thyroxine ( TT4 ) concentrations and thyroid gland volume ( by ultrasound ) . -
pubmed
Enhanced
cystatin C and
lysosomal protease expression following
6 - hydroxydopamine exposure . -
pubmed
Following
6 - OHDA exposure , the
caspase 3 - like inhibitor
Ac - DEVD - CHO significantly decreased
6 - OHDA induced cell
death . -
pubmed
In addition , alterations in expression of the
lysosomal cysteine and aspartic proteases ,
cathepsin B (
CB ) and
cathepsin D (
CD ) and the endogenous
cysteine protease inhibitor
cystatin C were observed utilizing immunocytochemical analysis at 24 , 48 , and 72 h following
6 - OHDA exposure . -
pubmed
Moreover , Western blot analysis confirmed a significant increase in protein expression for
CB and
CD at 72 h and a temporal and concentration dependent increase in
cystatin C in response to
6 - OHDA . -
pubmed
Cells treated with
pepstatin A , an inhibitor for CD , showed a significant decrease in cell
death , however ,
CA-074ME , a specific inhibitor for
CB , failed to protect cells from
6 - OHDA induced cell
death . -
pubmed
Thus , these results suggest that apoptosis induced by
6 - OHDA exposure is mediated in part through
caspase 3 activation and
lysosomal protease CD . -
pubmed
Effects of
aprotinin on
plasma coagulation kinetics determined by thrombelastography : role of
Factor XI . -
pubmed
Thrombelastography has been utilized to monitor coagulation in these settings , and
aprotinin delays clot initiation , presumably by inhibiting
kallikrein ; however ,
aprotinin also inhibits
Factor XI (
FXI ) , a contact system protein . -
pubmed
Thus , it was hypothesized that
celite - activated thrombelastography coagulation kin - etics would be decreased via
aprotinin - mediated
FXI inhibition . -
pubmed
Exposure of celite - activated ,
prekallikrein - deficient plasma to
aprotinin 0 , 200 , 400 or 800 KIU / ml resulted in R values of 366 + /- 15 , 630 + /- 64 , 698 + /- 46 and 850 + /- 47 s , respectively , which were significantly different from one another ( P & lt ; 0.05 ) . -
pubmed
There were no significant differences in R values between
tissue factor - activated ,
prekallikrein - deficient plasma samples with 0 or 800 KIU / ml
aprotinin . -
pubmed
CONCLUSIONS : These data support a role for the inhibition of
FXI as the mechanism for
aprotinin - mediated delayed contact system clot initiation determined by thrombelastography . -
pubmed
We examined the mechanism of
Al - induced
citrate release by studying the relationship between
citrate release and expression of the
mitochondrial citrate synthase (
mCS ) gene in three leguminous trees . -
pubmed
Lanthanum did not alter the expression level of
mCS . -
pubmed
We studied the correlation between serum
interleukin-2 (
IL-2 ) and
tumor necrosis factor - alpha (
TNF - alpha ) levels and the response to
89SrCl2 therapy . -
pubmed
After treatment with
89SrCl2 ,
IL-2 levels increased and
TNF - alpha levels decreased , with maximal changes at the fourth month after therapy . -
pubmed
After comparing the serum levels of
IL-2 and
TNF - alpha between responders and nonresponders , we found that these variables did not differ before
89SrCl2 therapy but differed significantly ( P & lt ; 0.05 ) after therapy . -
pubmed
Further studies are needed to elucidate the roles of
IL-2 and
TNF - alpha in the response to
89SrCl2 therapy and to evaluate their usefulness as indicators of
89SrCl2 efficacy . -
pubmed
Responses of neurons containing
VGLUT3 /
nNOS -
cGMP in the rVLM to cardiac stimulation . -
pubmed
Colocalization of
neuronal nitric oxide synthase , soluble
guanylyl cyclase and
c - Fos or
vesicular glutamate transporter 3 was noted following
bradykinin stimulation . -
pubmed
Immediately following exposure , the cytosol accounted for 40 % of the body burden in insects from both sites ; however , 89 + /- 2 % of the cytosolic
Cd was associated with
metallothionein - like proteins (
MTLP ) in insects from the contaminated site , compared to 60 + /- 0 % in insects from the reference site . -
pubmed
Results suggested that differences in exposure history had no influence on the bioaccumulation of
Cd , but did affect the concentrations of
Cd bound to
MTLP during
Cd exposure in these insects . -
pubmed
This suggests that , at high
phosphate doses , activation of the
proton pump is induced by the longer lasting proton influx acidifying the cytoplasm . -
pubmed
The increased
H (+ ) efflux due to the
proton pump could partially compensate protons taken up via the proton -
phosphate cotransport system . -
pubmed
CONTEXT : Despite the importance of
dihydrotestosterone in
androgen action , polymorphisms in the genes for the two isoforms of
5alpha - reductase (
SRD5A1 and
SRD5A2 ) have not been evaluated as risk factors for
polycystic ovary syndrome (
PCOS ) . -
pubmed
The
Leu allele of the Val89Leu variant in
SRD5A2 was associated with protection against
PCOS ; this allele is known to modestly reduce
5alpha - reductase activity . -
pubmed
Results from the present study suggest , for the first time , that
PLC attenuates
forebrain ischaemia - induced
neuronal injury , oxidative stress and energy depletion in the hippocampal
CA1 region . -
pubmed
A highly efficient synthesis of potent and selective
butyrolactam inhibitors of
11beta - HSD1 . -
pubmed
A convergent synthesis of structurally novel
butyrolactam 11beta - HSD1 inhibitors is described . -
pubmed
Using a yeast two - hybrid screen ,
NECAB2 , a neuronal
calcium binding protein , was identified as a binding partner for the
adenosine A ( 2A ) receptor (
A ( 2A ) R ) interacting with its C - terminal domain . -
pubmed
Sequence analysis of the tagged Tn917 - interrupted genes in these mutants indicated the participation in pathogenesis of the transcriptional regulatory proteins homologous to
GidA and
MerR ; the metabolic enzymes
asparagine synthetase A and
alpha - acetolactate synthase ; the ABC transport system of
glutamine and a
calcium - transporting ATPase ; the
dltA locus involved in alanylation of teichoic acids ; and hypothetical proteins containing EAL and Eis domains , among others . -
pubmed
The anti -
VEGF antibody
bevacizumab enhances the response rate and prolongs disease control when added to
interferon - alpha . -
pubmed
5 - Nitrobenzo [ b ] thiophene-2 - carboxylic acid 1 is converted to the corresponding
hydrazide 2 by the reaction with
hydrazine hydrate and then to the
azide 3 with
nitrous acid , followed by thermal rearrangement , cooling , and trapping in one
pot reaction . -
pubmed
Nitrogenase model complexes
[ Cp * Fe ( mu - SR ( 1 )) 2 ( mu - eta ( 2 )- R ( 2 ) N = NH ) FeCp * ] ( R(1 ) =
Me ,
Et ; R(2 ) =
Me ,
Ph ;
Cp * = eta ( 5 )- C5Me5 ): synthesis , structure , and catalytic
N - N bond cleavage of
hydrazines on
diiron centers . -
pubmed
The reactions of
[ Cp * Fe ( mu - SR1 ) 3FeCp * ] ( Cp * = eta5 - C5Me5 ;
R1 =
Et ,
Me ) with 1.5 equiv
R2NHNH2 (
R2 =
Ph ,
Me ) give the
mu - eta2 - diazene diiron thiolate - bridged complexes
[ Cp * Fe ( mu - SR1 ) 2 ( mu - eta2 - R2N NH ) FeCp * ] , along with the formation of
PhNH2 and
NH3 . -
pubmed
Aortic
inflammation , as assessed by hybrid
FDG - PET /
CT imaging , is associated with enhanced aortic stiffness in addition to concurrent
calcification . -
pubmed
PURPOSE : To analyse the relationship between : ( i ) aortic
pulse wave velocity ( PWV ) , an index of aortic stiffness with strong prognostic significance , and ( ii )
aortic calcification and
inflammation , which were quantified by hybrid imaging with X - ray computed tomography (
CT ) and
( 18 ) F - fluorodeoxyglucose (
FDG ) positron emission tomography ( PET ) . -
pubmed
CONCLUSION : Aortic
inflammation , assessed by hybrid
FDG - PET /
CT imaging , is associated with an enhanced aortic stiffness , in addition to the concurrent impact of
calcifications . -
pubmed
Expression of the
P2X7 receptor increases the
Ca2 + content of the endoplasmic reticulum , activates
NFATc1 , and protects from apoptosis . -
pubmed
Here we show that
P2X ( 7 ) transfection increased the ability of the endoplasmic reticulum to accumulate , store , and release
Ca ( 2 + ) . -
pubmed
This caused a larger agonist - stimulated increase in cytosol and mitochondrial
Ca ( 2 + ) in
P2X ( 7 ) transfectants than in mock transfected cells . -
pubmed
P2X ( 7 ) transfectants survived and even proliferated in serum - free conditions and were resistant to apoptosis triggered by
ceramide ,
staurosporin , or intracellular
Zn ( 2 + ) chelation . -
pubmed
Whole - body
fluorine-18 fluorodeoxyglucose positron emission tomography and
CT (
FDG - PET /
CT ) performed 15 days after right nephrectomy showed intense
FDG uptake in the aforementioned lesions ( lung and pleural nodules , mediastinal lymph nodes ) . -
pubmed
FDG - PET /
CT was considered useful for evaluating distant
metastases and thus portending the aggressive nature of sarcomatoid
RCC . -
pubmed
RESULTS :
Neocytolysis ensued that most evident from a rapid rise in
serum ferritin as the
iron from young RBC was transferred back to stores . -
pubmed
The contents of
trehalose constitutively accumulated in the
TPS1- and
TPS2 - overexpressing triple deletion
strains were higher than that in the original triple deletion
strain . -
pubmed
High
trehalose accumulation and growth activity were observed in the
TPS2 - overexpressing triple deletion
strain after
ethanol stress induction . -
pubmed
Based on these results , we have explored the molecular mechanisms underlying circadian oscillation and phase response to light of the master
clock located in the dorsomedial subdivision of the suprachiasmatic nucleus (
SCN ) and found various proteins closely related to phase response such as
BIT /
SHPS-1 and those of circadian oscillation , some of which are involved in protein -
tyrosine phosphorylation . -
pubmed
On the other hand , we have presented several lines of evidence that the ventrolateral subdivision of the
SCN includes not only the control center of energy supply to the brain , but also that of homeostasis such as blood
glucose , blood pressure ,
water balance , and body temperature . -
pubmed
We have also shown that besides these functions , the latter subdivision is involved in the regulations of hormone secretions such as
insulin ,
glucagon ,
corticosterone and
vasopressin . -
pubmed
Results from molecular docking and MD simulations demonstrate that nine PPIs adopt two different conformations ( extended and U -
bend structures ) at the binding sites and position themselves far above the
heme of
2C9 . -
pubmed
Interestingly , at the binding sites of all PPI -
CYP2C9 complexes except for
Lan /
CYP2C9 , there are
hydrogen - bonding networks made of PPIs , water molecules , and some residues of
2C9 . -
pubmed
However , in the case of
Lan/
2C9 , the hydrophobic interactions are more important than the electrostatic interactions for stabilizing the binding site . -
pubmed
Bax regulates production of
superoxide in both apoptotic and nonapoptotic neurons : role of
caspases . -
pubmed
A
Bax- and , apparently , mitochondria - dependent increase in
superoxide (
O ( 2 ) ( - ) ) and other reactive
oxygen species ( ROS ) occurs in apoptotic superior cervical
ganglion ( SCG ) and cerebellar granule ( CG ) neurons . -
pubmed
We used the
O ( 2 ) ( -)-sensitive dye
MitoSOX to monitor
O ( 2 ) ( - ) in neurons expressing different levels of
Bax and
mitochondrial superoxide dismutase (
SOD2 ) . -
pubmed
Basal and apoptotic
O ( 2 ) ( - ) levels in both SCG and CG neurons were reduced in
SOD2 wild - type ( WT ) cells having lower
Bax concentrations . -
pubmed
A
caspase inhibitor inhibited
O ( 2 ) ( - ) in both apoptotic and nonapoptotic SCG neurons . -
pubmed
O ( 2 ) ( - ) production increased when WT , but not
Bax - null , SCG neurons were permeabilized and treated with active
caspase 3 . -
pubmed
O ( 2 ) ( - ) levels in nonapoptotic
caspase 3 - null SCG neurons were lower than in WT cells but not as low as in
caspase inhibitor - treated cells . -
pubmed
These data indicate that
Bax lies upstream of most
O ( 2 ) ( - ) produced in neurons , that
caspase 3 is required for increased
O ( 2 ) ( - ) production during neuronal apoptosis , that
caspase 3 is partially involved in
O ( 2 ) ( - ) production in nonapoptotic neurons , and that other
caspases may also be involved in
Bax - dependent
O ( 2 ) ( - ) production in nonapoptotic cells . -
pubmed
Influence of
CYP2C8 * 13 and
CYP2C8 * 14 alleles on
amiodarone N - deethylation . -
pubmed
Effects of an N - type
calcium antagonist on
angiotensin II - renin feedback . -
pubmed
RESULTS :
Valsartan increased the plasma
angiotensin II (
Ang II ) level in a dose - dependent manner . -
pubmed
Cilnidipine suppressed the increase in
plasma renin activity and plasma
Ang II levels induced by
valsartan , but
amlodipine did not . -
pubmed
CONCLUSIONS : The results of this study suggest that the suppressive effect of
cilnidipine on the
valsartan - induced increase in
RAS activity can be partly explained by its sympatholytic action mediated by
N - type calcium channel blockade , and that combined administration of
cilnidipine and
valsartan might provide a synergistic therapeutic effect . -
pubmed
To further explore the role of dcEFs in regulating endothelial cells , we analyzed the endothelial cell proliferation and secretion of
nitric oxide (
NO ) ,
endothelin-1 (
ET-1 ) in response to dcEFs of physiological strength . -
pubmed
The results also showed dcEFs did not affect cell proliferation , but affected the productions of
NO and
ET-1 . -
pubmed
When COCs were cultured with
FSH ,
Fshr mRNA expression was immediately decreased within 5 h , whereas
Ptger2 ,
Ptger4 , and
Ptgs2 expression levels were significantly increased in cumulus cells in the culture containing
FSH for 5 or 10 h. The
PTGS2 inhibitor
NS398 significantly suppressed not only
PGE2 secretion at any culture time point but also
Areg ,
Ereg , and
Tace /
Adam17 expression in cumulus cells at 10 and 20 h but not at 1 or 5 h. During the early culture period , phosphorylation of
MAPK3 and
MAPK1 (
MAPK3 / 1 ) was not affected by
NS398 ; however , at 10 and 20 h , phosphorylation was suppressed by the drug . -
pubmed
Furthermore , down - regulations of
MAPK3 / 1 phosphorylation and expression of the target genes by
NS398 was overcome by the addition of either
PGE2 or
EGF . -
pubmed
FSH - induced cumulus expansion and meiotic progression to the MII stage were also suppressed by
NS398 , whereas these effects were also overcome by addition of either
PGE2 or
EGF . -
pubmed
More trials with carefully designed outcome measures are needed , especially with other modern combinations , including
mTOR inhibitors and/or
belatacept . -
pubmed
Activation of
Rice nicotianamine synthase 2 (
OsNAS2 ) enhances
iron availability for biofortification . -
pubmed
Activation of
iron deficiency - inducible
OsNAS2 resulted in a rise in
Fe content ( 3.0 - fold ) in mature seeds . -
pubmed
One possible cause of
NO deficiency is increased levels of endogenous
NO synthase inhibitors , in particular
asymmetric dimethylarginine (
ADMA ) . -
pubmed
At the end of the procedure , lung tissue levels of
thiobarbituric acid reactive substances ( TBARS ) ,
reduced glutathione (
GSH ) ,
superoxide dismutase (
SOD ) , and
catalase (
CAT ) were determined . -
pubmed
The most frequent
epidermal growth factor receptor mutation we detected was a deletion in exon 19 ( 60.3 % , 38 patients ) , followed by an L858R
amino acid substitution in exon 21 ( 27 % , 17 patients ) . -
pubmed
On multivariate analysis using the Cox proportional hazards model , the presence of
HLN metastasis ( P = 0.002 ) , along with a large number ( & gt ; or = 4 ) of regional lymph node
metastases ( P = 0.003 ) , and nonuse of
oxaliplatin - based chemotherapy ( P = 0.005 ) were identified as independent risk factors for shorter survival . -
pubmed
Blood biochemical markers measured at baseline and at the end of the study included :
triglycerides (
TG ) ,
cholesterol ,
HDL - C ,
LDL - C ,
VLDL - C ,
ApoB100 ,
ApoAI ,
C - reactive protein (
CRP ) , and
fibrinogen . -
pubmed
Serum total
cholesterol (
TC ) decreased significantly in the treatment groups compared with the control group ( P & lt ; 0.005 ) . -
pubmed
The
BRAF(V600E ) inhibitor
vemurafenib was used to confirm targets in
BRAF(V600E)-positive
melanoma cell lines and in
tumors from
melanoma patients undergoing inhibitor treatment . -
pubmed
The primary influence of gender was structure / geometry in bone growth and mechanical properties , whereas the mineral / matrix composition and
hydroxyproline content of bone were influenced primarily by the oim
collagen mutation . -
pubmed
The concentrations of total
PCBs in the
2,4 - D were in the range of
16.1 and 8023ngkg(-1 ) , and the WHO - TEQ values of the
PCBs were between 0.057 and 108.3ng WHO - TEQkg(-1 ) , while total
PCBs were between 1486 and 47342ngkg(-1 ) . -
pubmed
It was found that Garsi (
GR ) ( IC50=4.9 + -0.2 mg / L by
ABTS assay ) and Zaghwani ( ZG ) ( IC50=3.9 + -0.2 mg / L by
ABTS assay ) varieties exhibited the highest antioxidant activity . -
pubmed
Both diets decreased serum total
cholesterol ,
LDL -
cholesterol and
HDL - cholesterol levels , but
D1 had a greater effect on total
cholesterol and
LDL - cholesterol levels ( P< ; 0 001 and P< ; 0 05 , respectively ) . -
pubmed
D1 increased colonic fermentative activity , as judged from the higher ( P< ; 0 001 ) breath
hydrogen levels recorded . -
pubmed
We administered the same amount of the fluorescent dye ,
Nile red , into the middle ear in two forms : loaded in
PHEA polymersomes (
NP group ) or diluted in
ethanol ( NR group ) . -
pubmed
Although
Abeta hypothesis prevails in the ethiology of
Alzheimer ' s Disease (
AD ) , it has become increasingly evident that disturbances in cerebral
glucose metabolism is an invariant pathophysiological feature of
AD and may provide an ubiquituos mechanism underlying the pathogenesis of
AD . -
pubmed
In addition ,
paeoniflorin increased
glutathione level and restored the activity of
glyoxalase I to almost the control level . -
pubmed
Additionally , the
nitric oxide and
nuclear respiratory factor 1 levels were significantly increased by
paeoniflorin , suggesting that
paeoniflorin may induce mitochondrial biogenesis . -
pubmed
Paeoniflorin treatment decreased the levels of proinflammatory
cytokines such as
TNF - alpha and
IL-6 . -
pubmed
CONCLUSIONS : These findings indicate that
paeoniflorin might exert its therapeutic effects via upregulation of
glyoxalase system and mitochondrial function . -
pubmed
In spite of the low response rate ,
IFN - alpha therapy has the advantage of having a limited duration and being effective even after therapy , as demonstrated by a much higher incidence of
HBsAg clearance in responders to
IFN - alpha than in naturally occurring inactive
HBsAg carriers . -
pubmed
RESULTS : We identified a de novo missense mutation in
KCNB1 that encodes the KV 2.1 voltage - gated
potassium channel . -
pubmed
The percentage of patients meeting each criterion of the AHA was as follows : 46 % for
abdominal obesity ; 44 % for
hypertriglyceridemia or
cholesterol - lowering medication use ; 26 % for low
high - density lipoprotein cholesterol or being on a lipid - lowering medication ; 45 % for
hypertension ; and 20 % for high fasting
glucose or anti - diabetic medication use . -
pubmed
Basal tears were collected using a microcapillary tube and assayed for concentration and activity of the secretory
phospholipase A2 enzyme and concentration of the lipid
aldehyde malondialdehyde . -
pubmed
Secretory
phospholipase A2 enzyme activity in tears was associated with higher levels of
malondialdehyde ( R = 0.65 , p = 0.01 ) and shorter NISDT ( R = 0.84 , p = 0.001 ) . -
pubmed
Early postinfarction
ST2 levels and change over 24 weeks are related to
infarct extent and remodeling to a similar extent as
NT - proBNP and
aldosterone , and
ST2 may have a significant pathophysiological role in these postinfarction processes . -
pubmed
Estrogen - related Receptor beta Reduces the Subnuclear Mobility of
Estrogen Receptor alpha and Suppresses
Estrogen - dependent Cellular Function . -
pubmed
ERR has three subtypes ( alpha , beta , and gamma ) expressed in
estrogen - sensitive organs , including ovary , breast , and brain . -
pubmed
No endogenous ligands of
ERRs have been identified , but these receptors share a common DNA element with
ERalpha and control
estrogen - mediated gene transcription . -
pubmed
Recent evidence suggests a role of
ERRs in
estrogen - related pathophysiology , but the detailed mechanisms of
ERR functions in
estrogen - related tissues are unclear . -
pubmed
Using live - cell imaging with fluorescent protein labeling , we found that only
ERRbeta among the
ERRs exhibits a punctate intranuclear pattern overlapping with
ERalpha following
17beta - estradiol ( E2)-stimulation . -
pubmed
These results provide strong evidence for a suppressive effect of
ERRbeta on
estrogen signaling through reduction of the intranuclear mobility of
ERalpha . -
pubmed
The findings further suggest a unique inhibitory role for
ERRbeta in
estrogen - dependent cellular function such as
cancer cell proliferation . -
pubmed
Genistein increases
estrogen receptor beta expression in
prostate cancer via reducing its promoter methylation . -
pubmed
Genistein has protective effects against
prostate cancer (
PCa ) but whether this protection involves an
estrogen receptor ( ER ) beta dependent mechanism has yet to be elucidated . -
pubmed
However , the ability of
genistein to reverse
ER - beta promoter hypermethylation in
PCa has not been studied . -
pubmed
In addition , there are great discrepancies among studies that examined the effect of
genistein on
ER - beta gene expression . -
pubmed
We also examined the role of
ER - beta in mediating the preventive action of
genistein . -
pubmed
Genistein induced phosphorylation , nuclear translocation and transcriptional activity of
ER - beta in all three
PCa cell lines . -
pubmed
In conclusion ,
genistein and
ER - beta act together to prevent
PCa cell proliferation ;
genistein increases
ER - beta levels via reducing its promoter methylation and
ER - beta , in turn , mediates the preventive action of
genistein . -
pubmed
Arnebin-1 promotes angiogenesis by inducing
eNOS ,
VEGF and
HIF-1alpha expression through the
PI3K - dependent pathway . -
pubmed
In this study , in an aim to elucidate these mechanisms of action of
arnebin-1 , we investigated the effects of
arnebin-1 on the
VEGF receptor 2 (
VEGFR2 ) and the
phosphoinositide 3 - kinase (
PI3K)-dependent signaling pathways in HUVECs treated with
VEGF by western blot analysis . -
pubmed
We found that the activation of the
VEGFR2 signaling pathway induced by
VEGF was enhanced by
arnebin-1 . -
pubmed
Arnebin-1 promoted endothelial cell proliferation , migration and tube formation through the
PI3K - dependent pathway . -
pubmed
Moreover ,
Arnebin-1 significantly increased the
eNOS ,
VEGF and
HIF-1alpha expression levels in the HUVECs and accelerated the healing of
diabetic wounds through the
PI3K - dependent signaling pathway . -
pubmed
The molecular mechanism of
polygalasaponin F - mediated decreases in
TNFalpha : emphasizing the role of the
TLR4 -
PI3K /
AKT -
NF - kappaB pathway . -
pubmed
The results indicated that inhibition of
toll - like receptor ( TLR ) 4 (
CLI-095 1 mug / ml ) ,
phosphatidylinositol 3 - kinase (
PI3 K ) (
Ly294002 10 muM ) or
IkappaBalpha phosphorylation (
Bay11 - 7082 10 muM ) completely prevents the release of
TNFalpha induced by LPS without affecting cell viability and attenuated the nuclear translocation of
p65 stimulated by LPS . -
pubmed
The crystal and mol - ecular structures of the title salt ,
C8H8N3S2 ( + ) Cl (- ) , ( I ) , and salt
hydrate ,
C8H7ClN3S2 ( + ) Cl (- ) H2O , (
II ) , are described . -
pubmed
The common feature of the crystal packing in ( I ) and (
II ) is the formation of charge - assisted
amino - N - H Cl (- ) hydrogen bonds , leading to helical chains in ( I ) and zigzag chains in (
II ) . -
pubmed
In (
II ) , the chains are linked into a layer by charge - assisted water -
O - H Cl (- ) and water -
O - H O(water )
hydrogen bonds with charge - assisted
iminium - N (+)- H O(water )
hydrogen bonds providing the connections between the layers to generate the three - dimensional packing . -
pubmed
In (
II ) , the
chloride anion and water mol - ecules are resolved into two proximate sites with the major component being present with a site occupancy factor of 0.9327 ( 18 ) . -
pubmed
gondii infection (
TOX -
IgG ,
TOX -
IgM ) was performed , along with individual spermiograms and determinations of
follicle - stimulating hormone (
FSH ) and
testosterone serum levels . -
pubmed
gondii infection on sperm characteristics ( ejaculate quantity , sperm count , motility , morphology ) and serum levels of
FSH or
testosterone were found . -
pubmed
Despite their phylogenetic divergence , the geographically separate Calescamantes populations were highly similar in their predicted metabolic capabilities and core gene content , respiring
O2 , or oxidized
nitrogen species for energy conservation in distant but chemically similar hot
springs . -
pubmed
SIRT6 , a member of the mammalian sirtuins family , functions as a mono -
ADP -
ribosyl transferase and
NAD(+)-dependent deacylase of both
acetyl groups and long - chain
fatty acyl groups . -
pubmed
Glu106 targeted inhibitors of
ORAI1 as potential
Ca2 + release - activated Ca2 + ( CRAC ) channel blockers - molecular modeling and docking studies . -
pubmed
Calcium release - activated calcium modulator 1(
ORAI1 ) is an integral component of the
calcium release - activated calcium channel ( CRAC ) channel complex and plays a central role in regulating
Ca2 + concentrations in T - lymphocytes . -
pubmed
In this study , we have used a high - throughput screening approach to inhibit the binding of
Ca2 + toward
ORAI1 and the interactions are verified through induced fit docking . -
pubmed
The results hint that these compounds act by possibly binding with , and thereby blocking
Ca2 + -binding with
ORAI1 ( E106 ) . -
pubmed
We have thus identified a novel class of highly stable , potential lead compounds that directly bind with the selectivity filter region E106 and block
Ca2 + binding on
ORAI1 . -
pubmed
Two quantities , the
NM - related index ( RNM ) and the binding potential of the radiotracer
[ 18F ] FE - PE2I to DAT ( BPND ) in
SN , were measured for each subject using MRI and PET , respectively . -
pubmed
Development and validation of a sensitive and selective LC -
MS /
MS method for determination of
tacrolimus in oral fluids . -
pubmed
In this study , we report the development and validation of a rapid , sensitive and selective liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) method for quantification of
tacrolimus in OF . -
pubmed
Chromatographic separation were achieved on an Acquity UPLC BEH
C18 column by a gradient elution using 2mM
ammonium acetate/0.1 % ( v / v )
formic acid in
water ( mobile phase A ) and in
methanol ( mobile phase B ) with a 2.2min chromatographic run time . -
pubmed
Eighteen - month - old date
palm plants were subjected to severe salt ( 48 g / L
NaCl ) and drought ( 82.5 g / L
PEG or no irrigation ) conditions for one month . -
pubmed
Transketolase was significantly changed only in drought stress with
PEG . -
pubmed
Palladium - catalyzed heteroannulation of
[ 60 ] fullerene with
phenols has been developed as a practical and efficient protocol for the one -
pot synthesis of various
[ 60 ] fullerene - fused benzofurans . -
pubmed
If the incident energy spectrum is known , the dose measured in the PSD 's
polystyrene sensitive volume can be converted to score dose in any other media such as air , water or soft tissues using the large cavity theory (
LCT ) . -
pubmed
Molecular docking studies revealed that all compounds except
PGG showed binding energy values ranging from -10.30 to -8.00kcal / mol while the binding energy of
galantamine , a known
AChE inhibitor , was -9.53kcal / mol ; they inhibited the
AChE by binding into the ligand pocket with the similar binding pattern to that of
galantamine by interacting with
Glu199 of
AChE. -
pubmed
The interaction between
AChE and
PGG was further evaluated with the ITC method and the results indicated that the
PGG - AChE interaction was relevant to
AChE concentration . -
pubmed
Rate of
oxygen consumption decreased by 10 % from IS to
SS ( IS : 37.9 + - 5.68 ml kg-1 min-1 , SS : 34.1 + - 5.07 ml kg-1 min-1 , P & lt ; 0.0001 , ES 3.05 ) . -
pubmed
The neurobiological mechanisms underlying antidepressant effects of
CHM , including enhancement of
monoamine , dehyperactivation of hypothalamic - pituitary - adrenal axis , neuroimmune response , and
neurotrophic factors are discussed . -
pubmed
Extracellular
ATP is perceived by a plant purinoceptor ,
P2 receptor kinase 1 (
P2K1 ) , inducing downstream signaling for defense responses . -
pubmed
Therefore , enhancing the sensitivity and effect of radiotherapy with
propranolol , a
beta - adrenoceptor antagonist , could reduce
tumor growth . -
pubmed
Decreased expression of
NF - kappaB was also observed in treatment groups where both
propranolol and radiation were used , leading to the reduction of
COX-2 ,
EGFR , and
VEGF expression compared to that in the other groups . -
pubmed
Conclusion : The present study indicated that
propranolol potentiates the antitumor effects of radiotherapy in
gastric cancer by inhibiting
NF - kappaB expression and its downstream genes :
VEGF ,
EGFR , and
COX-2 . -
pubmed
Anti - mitochondrial drugs (
oligomycin ,
casiopeina II - gly , and
methoxy - TEA ) and
celecoxib , at doses used to block
OxPhos , significantly decreased
TN cancer cell proliferation ( IC50 = 2 - 20 muM ) , migration capacity ( 10 - 90 % ) , and invasiveness ( 25 - 65 % ) . -
pubmed
Methyl salicylate elicited emissions of
benzenoids ( BZ ) ,
monoterpenes ( MT ) and
fatty acid derived compounds (
LOX products ) . -
pubmed
Although the share of
LOX products was similar across
MeSA treatments , some
LOX products responded differently to
MeSA concentration , ultimately resulting in unique VOC blends . -
pubmed
It is predicted to be localized in the plasma membrane and may function in transmembrane
ion transport and/or as a
cell surface receptor . -
pubmed
Islet - Derived
eATP Fuels Autoreactive
CD8 + T Cells and Facilitates the Onset of
Type 1 Diabetes . -
pubmed
Extracellular
ATP (
eATP ) activates T cells by engaging the
P2X7R receptor . -
pubmed
eATP /
P2X7R signaling facilitates the onset of
autoimmune T1D by fueling autoreactive
CD8 + cells and therefore represents a novel targeted therapeutic for the disorder . -
pubmed
To interrupt neuronal transmission in the spinal defecation center , antagonists of
norepinephrine ,
dopamine , and/or
serotonin receptors were injected intrathecally at the
L6 -
S1 spinal level , where the spinal defecation center is located . -
pubmed
Intraluminal administration of
capsaicin , acting on the
transient receptor potential vanilloid 1 channel , caused transient propulsive contractions . -
pubmed
Capsaicin - induced contractions were blocked by preinjection of
D2 - like dopamine receptor and
5 - hydroxytryptamine subtype 2 and 3 receptor antagonists into the spinal defecation center . -
pubmed
sAA activity did not correlate with sAA concentration or salivary
cortisol , and a low correlation was observed between sAA concentration and salivary
cortisol ( r = 0.48 , p = 0.003 ) . -
pubmed
Methods : Five high - touch surfaces were cleaned and disinfected either according to the SOP (
alcohol - based cleaning and
chlorine - based disinfection ) or using
quaternary ammonium compounds - based disposable wipes (
MOP ) . -
pubmed
In this study , we examined the effectiveness of a hallmark
DOR agonist ,
SNC80 , in disease models reflecting diverse
headache disorders including :
chronic migraine ,
post - traumatic headache (
PTH ) ,
medication overuse headache by
triptans ( MOH ) , and opioid - induced
hyperalgesia (
OIH ) . -
pubmed
PTH was modeled by combining the closed head weight drop model with the
nitroglycerin model of chronic
migraine . -
pubmed
Engineered
Ssp DnaX inteins for protein splicing with flanking
proline residues . -
pubmed
We found that engineered
Ssp DnaX mini - intein and split inteins could carry out protein splicing with
proline at the +2 positions or at both -1 and +2 positions . -
pubmed
coli cells , the mini - intein ,
S1 split intein , and
S11 split intein spliced efficiently , whereas the S0 split intein did not splice with
proline at both -1 and +2 positions . -
pubmed
The
S1 and
S11 split inteins also trans - spliced efficiently in vitro with
proline at the +2 positions or at both -1 and +2 positions , but the S0 split intein trans - spliced inefficiently with
proline at the +2 position and did not trans - splice with
proline at both -1 and +2 positions . -
pubmed
Nine RCTs that compared hybrid
CR with usual care showed that hybrid
CR had greater improvements in peak
oxygen uptake ( 9.72 mL / kg per minute ; 95 % CI , 5.12 - 14.33 ) but not in hr - QOL ( 2 studies ; standardized mean difference : 0.67 ; 95 % CI , -0.20 to 1.54 ) . -
pubmed
Specially , the down - regulation of
nicotinamide (
NAM ) upon SK treatment indicated the suppression of the deacetylation of the
histone H3 on
lysine 56 residue ( H3K56 ) . -
pubmed
CONCLUSIONS :
UGT1A1 polymorphisms were associated with improved outcomes , despite
pazopanib interruption and dose reductions . -
pubmed
In
era of antibiotic resistance , antibacterial
silver nanoparticles are considered as potential alternative therapeutic agent to combat drug resistant pathogens . -
pubmed
Evolving a Unique Red - emitted Fluorophore with Optically Tunable
Hydroxy Group for Imaging
Nitroreductase in Cell , Tissue and Vivo . -
pubmed
The cell debris solutions and filter liquor from inducible and non - inducible samples were cultured with
3 - PBA and its intermediate metabolites at same condition , and the location and induction of
enzymes were analyzed by the degradation . -
pubmed
This study provided a promising method for studying the co - metabolic
enzymes of
3 - PBA degradation by fungi . -
pubmed
Polymorphic
alpha - Synuclein Strains Modified by
Dopamine and
Docosahexaenoic Acid Interact Differentially with
Tau Protein . -
pubmed
Our institution began using front - loaded
lorazepam in August 2017 secondary to a nation - wide shortage of intravenous (
IV )
diazepam . -
pubmed
CONCLUSION : Front - loaded
lorazepam was similar to front - loaded
diazepam in controlling
AWS symptoms . -
pubmed
PARP1 catalytic variants reveal branching and chain length - specific functions of
poly ( ADP - ribose ) in cellular physiology and stress response . -
pubmed
Poly ( ADP - ribose ) (
PAR ) molecules exhibit a remarkable heterogeneity in chain lengths and branching frequencies , but the biological significance of this is basically unknown . -
pubmed
Adjuvants are mixed with the immunizing antigen for the first two immunizations only , and Complete Freund 's adjuvant is only used with the first immunization ; subsequent immunizations are performed in
phosphate - buffered saline (
PBS ) or normal saline , with or without Incomplete Freund 's adjuvant . -
pubmed
The impacts of
TIG on the level of
inflammatory cytokines (
IL-1beta ,
TNF - alpha ,
IL-6 ,
IFN - gamma ,
IL-17 and
IL-10 ) , and the regulation of
OPG /
RANKL /
NF - kappaB pathways were determined by the ELISA and western blot , respectively . -
pubmed
Moreover ,
TIG markedly inhibited the expression of
p - IKK - alpha ,
p - IkappaB and
p - p65 , and decreased the ratio of
OPG /
RANKL in the synovial tissues . -
pubmed
CONCLUSION :
TIG possessed significant anti - RA activities on adjuvant - induced
arthritis , which might be ascribed to the regulation of
inflammatory cytokines IL-1beta ,
TNF - alpha ,
IL-6 ,
IFN - gamma IL-17 and
IL-10 , as well as inhibition of
OPG /
RANKL /
NF - kappaB signaling pathways . -
pubmed
The prepared
CuO /
ZnO - DSDSHNM was used over the
glassy carbon electrode (
GCE ) as the active material for
glucose detection and then coated by
Nafion to provide the proposed
Nafion /
CuO /
ZnO -
DSDSHNM /
GCE . -
pubmed
The achieved high - performance
glucose sensing based on
Nafion /
CuO /
ZnO - DSDSHNM /
GCE shows that both the material synthesis and the sensor fabrication methods have been promising and they can be used in future researches . -
pubmed
Asymmetric binding of the inhibitor
di ( adenosine-5 ' ) pentaphosphate (
Ap5A ) to
adenylate kinase . -
pubmed
The effect of binding
diadenosine pentaphosphate (
Ap ( 5 ) A ) to
adenylate kinase (
ATP : AMP phosphotransferase ;
EC 2.7.4.3 ) has been investigated by
( 31 ) P nuclear magnetic resonance . -
pubmed
The symmetric molecule , Ap(5)A , is a potent inhibitor of the
adenylate kinase reaction , 2
ADP right arrow over left arrow
ATP +
AMP . -
pubmed
The spectrum of
Ap ( 5 ) A bound to
porcine adenylate kinase shows five groups of signals centered at 10.9 , 11.9 , 20.5 , 22.7 , and 24.0 ppm ; the resonances at 11.1 ppm ( 1 - P and 5 - P ) and at 22.8 ppm ( 2 - P and 4 - P ) are now clearly split , indicating asymmetric binding of
Ap ( 5 ) A to the
enzyme . -
pubmed
The asymmetry is strikingly enhanced in
enzyme - bound
MgAp ( 5 ) A , which has resonances at 10.5 , 12.5 , 18.6 , 22.7 , and 25.6 ppm . -
pubmed
By the addition of
Mn ( 2 + ) to the
enzyme . -
pubmed
MgAp ( 5 ) A complex , the observed signals in increasing order of shifts were tentatively assigned to 1 - P , 5 - P , 4 - P , 3 - P , and 2 - P , where the 3- , 4- , and 5 - P positions correspond to the
ATP - binding site on the
enzyme . -
pubmed
The asymmetry introduced in the
phosphate chain of
enzyme . -
pubmed
MgAp ( 5 ) A is indicated by the
( 31 ) P chemical shift of 7 ppm between 2- and 4 - P , which is one of the largest thus far observed for
phosphate substrates bound noncovalently to
enzymes . -
pubmed
If this has been ruled out and if
oestrogens are elevated with low plasma
gonadotrophins , a feminizing
ovarian or adrenal tumour should be considered . -
pubmed
cholerae 0395 containing a
TnphoA insertion in a gene designated
viuA , lacks this 74 - kDa outer membrane protein and is unable to bind or utilize exogenous
ferric vibriobactin . -
pubmed
Introduction of a plasmid containing the complete
viuA coding sequence and 513 bp of upstream DNA into MBG14 restored
ferric vibriobactin utilization to the mutant . -
pubmed
Construction of
viuA::
TnphoA gene fusions allowed study of the regulation of
viuA expression by
iron . -
pubmed
cholerae grown in high- and low -
iron media revealed a monocistronic
viuA message that was negatively regulated by
iron at the transcriptional level . -
pubmed
cholerae by
fur ; additional regulatory factors , superimposed upon the fur system , may provide more precise control of individual
iron - regulated genes . -
pubmed
Because
pentoxifylline (
PTXF ) inhibits
TNF production , we hypothesized that
PTXF would ameliorate
IL-2 toxicity without compromising antitumor efficacy . -
pubmed
Upon completion of therapy , we found that
PTXF suppressed many of the
IL-2 - induced effects including
TNF production , lymphocytic infiltration of multiple organs ,
multiple organ edema ,
hepatic dysfunction ,
leukopenia , and
thrombocytopenia . -
pubmed
PTXF preserved antitumor efficacy while reducing the morbidity and mortality caused by
IL-2 treatment . -
pubmed
These data strongly support the use of
PTXF in extending the therapeutic index of
IL-2 in the treatment of
cancer . -
pubmed
The
dopamine (
D2 ) blocker ,
haloperidol , did not block
AMPH actions . -
pubmed
One group had large
HDL particle sizes and high
HDL -
cholesterol concentrations . -
pubmed
Another group had smaller
HDL particles and lower
HDL -
cholesterol concentrations , and
HDL sizes and
HDL -
cholesterol concentrations were significantly correlated . -
pubmed
In the RI strains ,
HDL sizes and
HDL -
cholesterol cholesterol concentrations clearly segregated with one or another of the progenitor strains , and RI
strain distributions showed a strong linkage to the
apolipoprotein ( apo ) A - II gene (
Apoa-2 ) . -
pubmed
In contrast ,
LDL -
cholesterol concentrations and particle sizes on FPLC did not show dichotomous distributions among the 10 inbred
strains . -
pubmed
In RI strains , the configuration of the
LDL FPLC profiles and
LDL -
cholesterol concentrations did resemble one or another of the progenitors in the majority of cases , but
LDLs of several RI
strains resembled neither progenitor strain in profile configuration , and
LDL -
cholesterol concentrations were both greater and smaller than those of progenitor
strains . -
pubmed
However ,
LDL particle diameters ( as judged by peaks of
LDL -
cholesterol profiles ) did segregate with progenitors in 29/33 ( 88 % ) of RI strains suggesting that a major gene may affect
LDL size . -
pubmed
Dexamethasone inhibits the induction of
monocyte chemotactic - activating factor production by
IL-1 or
tumor necrosis factor . -
pubmed
We examined the turn - over of synthesized MCAF mRNA that showed
DXS decreased the stability of
MCAF mRNA . -
pubmed
In addition , a nuclear run - off analysis revealed that
DXS also inhibited the transcription of IL-1- or
TNF - activated
MCAF genes . -
pubmed
Therefore , both the destabilization of
MCAF mRNA and the inhibition of transcription of the gene contribute to the decrease in the
MCAF mRNA steady state level by
DXS . -
pubmed
One porter developed high altitude
pulmonary edema , Grade
IV , at an altitude of 4,200 m , and was successfully treated with
oxygen ,
nifedipine and descent . -
pubmed
A second precolumn packed with
C18 - bonded
silica was used for the reconcentration of desorbed
clenbuterol prior to the analytical separation . -
pubmed
Urine , after 2 - fold dilution with buffer ( pH 7.4 ) , was loaded directly onto the immuno precolumn , where
clenbuterol was trapped by the immobilized
antibodies . -
pubmed
The sustained negative potential and the negative OFF response were not the M - wave ON and OFF responses of proximal retina , despite an overall resemblance in form and time course : 1 ) the M - wave was spatially tuned , whereas the
ERG components were not ; 2 )
tetrodotoxin (
TTX ) ( 3.8 - microM vitreal concentration ) did not alter the M - wave , but it reduced the
ERG OFF response ; 3 )
picrotoxin ( 0.14 mM , after
TTX ) enhanced the M - wave but did not affect the negative ERG ; and 4 )
2 - amino-4 - phosphonobutyric acid (
APB ; 0.95 mM ) removed the M - wave ON response , and
aspartate ( 43 mM ) removed the M - wave OFF response , in addition , while the sustained negative potential persisted . -
pubmed
The number of
amino acids between the two conserved
cysteine residues is intermediate between those of the
Ig V and C domains . -
pubmed
Likewise , a clear - cut synergism was observed when a low dose of the
dopamine receptor agonist
apomorphine ( 0.1 mg / kg ) , which did not per se affect motor activity , was combined with
MK-801 ( 1.5 mg / kg ) ; however , the synergism between
apomorphine and
MK-801 was less dramatic than that observed between
MK-801 and
clonidine . -
pubmed
Removing extracellular
Na + prevented the development of the negative slope in this voltage region , suggesting that
Na + can block
IK1 channels in a time- and voltage - dependent manner . -
pubmed
The time and voltage dependence of
Cs +-induced block of
IK1 was also examined . -
pubmed
In the absence of
Na + , inactivation of
IK1 could also be induced by both
Cs + and
Li + . -
pubmed
The degradation of
3H - TP60 in the absence of RNA synthesis was inhibited by
iodoacetamide , a
cysteine protease inhibitor , resulting in the accumulation of
3H - TP60 in the nucleus reaching about a threefold concentration in the cytoplasm . -
pubmed
The
NADPH - nitrate reductase enzymes formed in the above three complementing mixtures are similar to the wild - type enzyme in
sucrose density gradient profiles , molecular weight , substrate affinity , sensitivity to inhibitors and temperature , but show different ratios of associated enzyme activities . -
pubmed
The data suggest that
nitrate reductase consists of at least two protein subunits : a
nitrate - inductible subunit as reflected by inductible
NADPH - cytochrome c reductase , and a constitutive protein which is activated ( as indicated by the appearance of
flavine adenine dinucleotide , reduced form (
FADH ( 2 ))- and reduced
methyl viologen - nitrate reductase activities ) when it combines with the inductible subunit . -
pubmed
3 - bromoacetonyltrimethylammonium bromide , a
choline acetylase inhibitor . -
pubmed
[ Lack of activity of
diphenhydramine in the secretion of
prolactin and
insulin induced by
arginine ] . -
pubmed
Membranes prepared throughout in
N - ethylmaleimide , which yield upon detergent solubilization a predominant 13 - S dimeric species and which are not depleted of the nonreceptor
v - peptide ( Mr 43 000 ) by alkaline treatment , are relatively more ' rigid ' than normal membranes and do not show the anisotropy components characteristic of the monomeric receptor . -
pubmed
The influence of the v - peptide on the structural stability of the
receptor oligomeric forms and the
thiol - dependent interconversions are thus reflected in the time - dependent spectroscopic measurements . -
pubmed
[ Pro4 , Trp7,9 ] SP-(4 - 11 ) and a newly developed compound ,
[ Pro4 , Trp7,9,10 ] SP-(4 - 11 ) were shown to be potent antagonists of
substance P ( SP ) . -
pubmed
Water versus
acetone -
HCl extraction of
digitalis - like factor from guinea - pig heart . -
pubmed
The
enzyme was maximally activated , approximately 2 - fold , in the presence of 0.1 - 0.4 % ( w / v )
digitonin . -
pubmed
Induction of the
digitonin activated
clofibric acid glucuronyltransferase was observed following
phenobarbitone treatment in vivo ( 2.2 - fold ) , and to a lesser extent , following
beta - naphthoflavone treatment ( 1.3 - fold ) . -
pubmed
0.08 nmoles
glucuronide formed / min / mg microsomal
protein ) . -
pubmed
The data indicated that
clofibric acid glucuronyltransferase belongs to the neonatal cluster of enzymes and
clofibric acid is a group 2 substrate . -
pubmed
An unusual pattern of peptide - bound
lysine metabolism in
collagen from an infant with lethal
perinatal osteogenesis imperfecta . -
pubmed
It was subsequently shown that the apparent deficiency in
lysyl residues was due , at least in part , to the presence of unusually high levels of
allysine , a cross - link precursor formed from peptide - bound
lysine under the catalytic action of
lysyl oxidase . -
pubmed
The inhibitory effect of
MTX - poly ( Lys ) on
dihydrofolate reductase in vitro is increased nearly 100 - fold in the presence of excess heparin but remains less than that of free
MTX . -
pubmed
These observations raise the possibility that at least part of the
MTX - poly ( Lys )- heparin reaches cellular
dihydrofolate reductase in the form of macromolecular complexes that escape from entrapment in endocytotic structures . -
pubmed
Activities of
glycolytic enzymes in the pectoralis muscle showed similar patterns of development as those found for the aerobic enzymes , except for
hexokinase , which was low at all ages , indicating an adaptation for catabolism of both intracellular glycogen and plasma
fatty acids in preference to plasma
glucose . -
pubmed
Activities of anaerobic
glycolytic enzymes in the ventricles were low , but values for
hexokinase , which is involved in the phosphorylation of plasma
glucose , developed rapidly . -
pubmed
Values for
lactate dehydrogenase were also high , reflecting the capacity of the heart to catabolize plasma
lactate . -
pubmed
The average maximum
oxygen uptake for premigratory geese during flight , as indicated by values for
OGD , is calculated to be 484 ml
O2 / min ( or 208 ml O2.min-1.kg-1 ) . -
pubmed
ADP - ribosylation is a reversible
post - translational modification of proteins involving the addition of the
ADP -
ribose moiety of
NAD to an acceptor protein or
amino acid . -
pubmed
The reverse reaction , catalyzed by
ADP - ribosylarginine hydrolase , removes
ADP -
ribose , regenerating free
arginine . -
pubmed
IGF - I and
insulin both stimulated
3 - O - methylglucose uptake and
GLUT-4 translocation in a dose - dependent manner with a maximal effect six- to sevenfold above basal . -
pubmed
No additive effects of
IGF - I and
insulin on maximal
3 - O - methylglucose uptake were found . -
pubmed
In contrast ,
IGF - I bound to the partially purified
IGF - I receptor with an apparent affinity ( ED50 = 3.7 nM ) that was similar to the concentrations of
IGF - I which caused half - maximal activation of
3 - O - methylglucose uptake ( ED50 = 2.4 nM ) and
GLUT-4 translocation ( ED50 = 2.5 nM ) . -
pubmed
Our findings suggest that
IGF - I exerts its
insulin - like effects on
glucose uptake primarily through its own specific receptor and that the molecular events underlying
IGF - I and
insulin actions on
glucose uptake in skeletal muscle are similar , namely caused by a translocation of the
GLUT-4 transporter from an intracellular pool to the cell surface . -
pubmed
This was done by the determination of
progesterone production ,
aromatase activity and
cAMP accumulation after a 48 h incubation with PMSG ,
IGF - I and PMSG plus
IGF - I in cells treated with either
CT or PT . -
pubmed
Both
CT and PT treatment stimulated
PMSG - induced
progesterone production in granulosa cells after 48 h of culture with PMSG . -
pubmed
CT treatment also stimulated
aromatase activity in cells treated with PMSG and increased
cAMP secretion under basal conditions ( untreated cells ) and in PMSG treated cells . -
pubmed
Both
CT and PT increased the stimulation by
IGF - I of PMSG - induced
progesterone production after 48 h of culture with PMSG plus
IGF - I. -
pubmed
Furthermore ,
CT augmented the enhancement by
IGF - I of
PMSG - induced
aromatase activity and
cAMP accumulation . -
pubmed
In the absence of PMSG ,
CT did not increase steroidogenesis either alone or in the presence of
IGF - I within the time frame studied even though
CT was able to stimulate
cAMP accumulation in untreated and
IGF - I treated cells . -
pubmed
After concentration of
amoxicillin on a C8 cartridge , the cartridge was eluted on - line to a reversed - phase column packed with 5 microns Chromspher
C18 . -
pubmed
The
organophosphonate recruited chemical potential from the immune response that resembled the way these compounds recruit the catalytic power of the
serine hydrolases . -
pubmed
During this recruitment , a large proportion of the isolated
antibodies catalyzed the formation and cleavage of phosphonylated intermediates and subsequent
ester hydrolysis . -
pubmed
Furthermore ,
MN frequencies obtained from slides stained with May - Grunwald - Giemsa ( MGG ) and with the DNA
fluorochrome 4',
6 '- diamidino-2 - phenylindole (
DAPI ) were compared to check if the DNA - specific
DAPI facilitated a more accurate recording of
MN than the unspecific MGG . -
pubmed
Alachlor ( 7.5 - 20 micrograms / ml ) and
MMC ( 0.6 microM ) acted as pure clastogens without aneugenic activity , inducing exclusively KC- and CM - negative
MN . -
pubmed
VBL produced primarily KC- and CM - positive
MN , in accordance with its known mechanism of action . -
pubmed
A comparison between CM and KC data in the
VBL treatment suggested that some 7 % of KC - containing
MN may not be detected by the probe . -
pubmed
The frequencies of
MN were generally higher in slides stained with
DAPI than in those stained with MGG , especially in controls and
clastogen - treated cultures. -
pubmed
We also studied the induction of
7 - ethoxyresorufin - O - deethylase (
EROD ) in these
PCB - treated cells . -
pubmed
Microsequencing of the 50 - kDa band yielded an amino - terminal sequence of 38
amino acids identical to that deducted from the
CYP2D16 cDNA . -
pubmed
Sodium dodecyl sulfate -
polyacrylamide gel electrophoresis and Western blot analyses demonstrated greater expression of
CYP2D16 in microsomes from the inner zone (
zona reticularis ) of the adrenal cortex than from the outer zones , coinciding with the major site of adrenal xenobiotic metabolism . -
pubmed
Actin disruption inhibits
bombesin stimulation of
focal adhesion kinase (
pp125FAK ) in
prostate carcinoma . -
pubmed
UNLABELLED :
Jasplakinolide is a member of a new class of antitumor agents targeting the
actin cytoskeleton with activity against
prostate cancer . -
pubmed
We hypothesized that the neuropeptide
bombesin would activate
FAR in
prostate carcinoma , and that disruption of the
actin network would block
FAK activation and inhibit cell growth . -
pubmed
FAK phosphorylation was measured in cells stimulated with 0.01 - 10 nM
bombesin ; separate cells were pretreated 6 hr with 50 - 500 nM
Jas or CyE. -
pubmed
Cell lysates and anti -
FAK immunoprecipitates were subjected to
SDS - PAGE , Western blotting , and detection with anti -
actin or anti - phosphotyrosine . -
pubmed
Bombesin rapidly stimulated
tyrosine phosphorylation of
FAK in a dose dependent manner . -
pubmed
FAK phosphorylation was inhibited to near - basal levels ( 50 % of
bombesin stimulated ) by 500 nM
Jas ( 63 % ) and 500 nM
CyE ( 61 % ) . -
pubmed
CONCLUSIONS :
Bombesin stimulated
FAK in
prostate carcinoma cells . -
pubmed
Substrate
nucleotide - determined non - templated addition of
adenine by Taq DNA
polymerase : implications for PCR - based genotyping and cloning . -
pubmed
The Applied Biosystems PRISM fluorescence - based genotyping system as well as the Invitrogen TA Cloning vector system are influenced by the tendency of Taq DNA
polymerase to add an
adenine nucleotide to the 3 ' end of PCR products after extension . -
pubmed
Lysates of C57BL/6J - derived BMMC contained a
Ca2 + -dependent
PLA2 that was absorbed to a column conjugated with
anti - type IIA sPLA2 antibody and had a similar molecular mass of 14 kDa , as assessed by immunoblotting . -
pubmed
The soluble
mineralocorticoid receptor bound to an agonist acts as a transcription factor for several genes relevant to ion transport by kidney and colon epithelial cells and is a major regulator of
electrolyte and fluid homeostasis . -
pubmed
The isolated
mineralocorticoid receptor binds several adrenal
steroids , including
aldosterone and the major
glucocorticoids , with equal affinity . -
pubmed
Evidence is presented for the de novo synthesis of adrenal
corticosteroids in the brain which may , by paracrine regulation of central control mechanisms , be relevant for certain clinical and experimental forms of
hypertension characterized by low circulating levels of mineralocorticoids which respond to
mineralocorticoid receptor antagonists . -
pubmed
During the course of our analysis of
myo - inositol monophosphatase (
IMPase ) , a key enzyme of brain
inositol signaling , we found it also hydrolyzes
galactose 1 - phosphate (
Gal 1 - P ) , an intermediate of
galactose metabolism . -
pubmed
The ability of
IMPase to use both
inositol 1 - phosphates and
galactose 1 - phosphate equally as substrates is of considerable importance in determining
lithium 's mechanism of action . -
pubmed
Our current results suggest that during
lithium therapy , both
galactose and
inositol metabolic pathways can be simultaneously modulated through
lithium inhibition of
IMPase . -
pubmed
Enzyme studies with
Mg2 + ions as activators and with
Li + ,
Ca2 + ,
Mn2 + ,
Ba2 + ions as inhibitors demonstrate that
IMPase is a single enzyme possessing the ability to hydrolyze both
inositol monophosphates and
Gal-1 - P with equal efficiency . -
pubmed
Our results indicate that
lithium inhibition of
IMPases at clinically relevant concentrations , may modulate both
inositol and
galactose metabolism , and identifies yet another
carbohydrate pathway utilizing
IMPase . -
pubmed
On postoperative day 3 , if both a
pneumothorax and
AL were present , the
CT was placed to 10 cm
H2O of suction . -
pubmed
If a
pneumothorax was present without an AL , the
CT was returned to 20 cm
H2O of suction . -
pubmed
Oxidative modification of
glutamine synthetase by
2,2 '- azobis ( 2 - amidinopropane ) dihydrochloride . -
pubmed
To this end , we exposed
glutamine synthetase (
GS ) and the peptide melittin to solutions containing
2,2 '- azobis ( 2 - amidinopropane ) dihydrochloride (
AAPH ) , which is known to decompose in aqueous , aerobic solutions to yield
alkyl radicals and
alkylperoxides . -
pubmed
Under our conditions , pH 7.4 , 37 degrees C , the
AAPH - dependent formation of
alkylhydroperoxide increased linearly with time and led to 40 % inactivation of
GS in 1 h and to complete inactivation in 4 h. Complete inactivation was associated with the loss of 2 of 16
histidine residues , 6 of 17
tyrosine residues , 5 of 16
methionine residues , and all of the
tryptophan residues ( 2 residues ) per subunit . -
pubmed
Exposure of
GS to
AAPH led also to the generation of protein
carbonyl derivatives ( 0.34 mol / mol subunit ) and to formation of a significant amount ( 0.038 mol / mol subunits ) of quinoprotein derivatives . -
pubmed
To investigate the mechanism of
tryptophan modification , the
26 - amino - acid peptide ,
melittin , which contains one
tryptophan but no
histidine ,
tyrosine , or
methionine residues , was treated with
AAPH . -
pubmed
Stimulation with lipopolysaccharide and
IL-1beta resulted in the full induction of
IL-6 expression only if the cells were coincubated with
cAMP agonists ; this effect was attenuated by
protein kinase A inhibitors . -
pubmed
Inhibition of
NO - synthase by the intravenous administration of
L - NNA reduced significantly the amplitude of
neurotensin hyperaemia . -
pubmed
Pretreatment with
L - Arginine , the substrate of
NO - synthase reversed the hemodynamic , but not motility effects of
neurotensin . -
pubmed
Neuronal nicotinic receptors in
dementia with
Lewy bodies and
schizophrenia :
alpha - bungarotoxin and
nicotine binding in the thalamus . -
pubmed
AIM : To study antianginal effectiveness of
trimetazidine (
TMS ) in monotherapy and combined antianginal therapy ; to design approaches to prediction of its effect . -
pubmed
The proteins , which were named
grainin 1 and 2 , show a considerable structural similarity to
calcium - binding proteins , particularly within EF - hand motifs . -
pubmed
Each
grainin possesses three of these putative
calcium - binding sites . -
pubmed
Calcium - binding activity of
grainins was demonstrated by two independent methods . -
pubmed
Dependent variables for the study were total
cholesterol ( TC ) ,
triglycerides ( TG ) ,
high - density lipoprotein cholestrol (
HDL - C ) ,
low - density lipoprotein cholesterol (
LDL - C ) , and systolic (
SBP ) and diastolic (
DBP ) blood pressure . -
pubmed
Identification of
Cys140 in helix 4 as an exofacial
cysteine residue within the substrate - translocation channel of
rat equilibrative nitrobenzylthioinosine ( NBMPR )- insensitive nucleoside transporter rENT2 . -
pubmed
PCMBS caused reversible inhibition of
uridine influx by
rENT2 , but had no effect on
hENT1 ,
hENT2 or
rENT1 . -
pubmed
This difference correlated with the presence in
rENT2 of a unique
Cys residue (
Cys(140 ) ) in the outer half of TM4 that was absent from the other
ENTs . -
pubmed
Mutation of
Cys(140 ) to
Ser produced a functional protein (
rENT2 / C140S ) that was insensitive to inhibition by
PCMBS , identifying
Cys(140 ) as the exofacial
Cys residue in
rENT2 responsible for PCMBS inhibition . -
pubmed
Uridine protected wild - type
rENT2 against PCMBS inhibition , suggesting that
Cys(140 ) in TM4 lies within or is closely adjacent to the substrate - translocation channel of the transporter . -
pubmed
Attenuation of
hypothermia - induced platelet activation and platelet adhesion to artificial surfaces in vitro by modification of
hemoglobin to carry
S - nitric oxide and
polyethylene glycol . -
pubmed
The effects of
human hemoglobin (
Hb ) covalently modified to carry
S - nitric oxide (
NO ) functional groups (
SNO - Hb ) ,
polyethylene glycol (
PEG - Hb ) , and
SNO -
PEG - Hb on platelet activation were studied . -
pubmed
Platelet adhesion and aggregation were measured in a parallel glass plate chamber coated with unmodified
Hb ,
SNO - Hb ,
PEG -
Hb ,
SNO -
PEG -
Hb , and
collagen . -
pubmed
There was significantly less platelet binding of antibodies to
GPIIb - IIIa and
P - selectin with
SNO - Hb ,
PEG -
Hb , and
SNO - PEG - Hb compared with unmodified
Hb . -
pubmed
There was significantly less platelet attachment , adhesion , and aggregation on the
SNO - Hb ,
PEG - Hb and
SNO -
PEG -
Hb coated surfaces compared with unmodified
Hb - coated and -uncoated surfaces . -
pubmed
SNO - Hb ,
PEG - Hb , and
SNO -
PEG -
Hb induced less platelet activation at hypothermic temperature , and induced less platelet adhesion and aggregation on thrombogenic surfaces compared with unmodified
Hb . -
pubmed
The inhibitory effect may be derived from antiadhesive properties of
Hb , antiplatelet actions of
NO , and molecular barrier action of
PEG . -
pubmed
Investigation of the levels of
cyclins E , D and B by immunoblot analysis showed
cyclin B level was unaffected , whereas
cyclin D and E levels declined with
curcumin in both cell lines . -
pubmed
Investigation of
cyclin - dependent kinases ,
Cdk2 and
Cdc2 , showed activity of
Cdc2 , but not
Cdk2 , increased markedly in response to
curcumin . -
pubmed
In both cell lines , immunoblot analysis indicated that
curcumin caused induction of apoptosis as evidenced by cleavage of
PARP ,
caspase-3 , and reduction in
Bcl - XL levels . -
pubmed
Curcumin also stimulated the activity of
caspase-8 , which initiates
Fas signalling pathway of apoptosis . -
pubmed
O-15 water
CBF images can predict the effect of
Nicholin by assessing transient increases in cerebral blood flow (
CBF ) , thereby facilitating improvement in higher brain functions such as orientation . -
pubmed
Brain SPECT with
I-123 IMP can also identify " misery " perfused areas resulting from reduced
CBF and decreased vasoreactivity in the chronic phase . -
pubmed
The inclusion of
hexafluoroisopropanol in the cleavage solvent mixture did not improve yields for either
gA or WALP peptides . -
pubmed
For example , higher
estrogen levels , elevated
insulin levels , a greater concentration of growth factors in adipose tissue ,
hypertension ,
cholesterol metabolism abnormalities , and
immune malfunction are just some of the potential mechanisms that may increase
kidney cancer risk . -
pubmed
There was also an association between
BNP and male sex ( p = 0.008 ) , higher plasmatic
creatinine ( p = 0.01 , r = 0.25 ) , Iarger ventricular diameters ( p = 0.0001 ) and higher pulmonary
systolic pressure ( p = 0.001 , r = 0.44 ) . -
pubmed
The
FP region is composed of hydrophobic ,
glycine - rich residues that are essential for the initial penetration of the target cell membrane . -
pubmed
Primary management of
post - radiation bone lesions include conservative modalities such as saline irrigations , antibiotics during infectious episodes , topically applied antiseptics , gentle sequestrectomy and removal of visibly loosened bone elements as well as treatment with
hyperbaric oxygen ( HBO ) . -
pubmed
Human serum and glucocorticoid - inducible kinase - like kinase (
SGKL ) phosphorylates
glycogen syntheses kinase 3 beta (
GSK-3beta ) at
serine-9 through direct interaction . -
pubmed
Furthermore , the kinase activity of wild - type
SGKL was required for the in vitro phosphorylation of a
GSK-3 crosstide fusion protein at
serine-21/9 as demonstrated with a
Phospho -
GSK-3alpha /
beta ( Ser21 / 9 ) specific antibody . -
pubmed
The present results provide strong evidences that
SGKL could utilize
GSK-3beta as a direct downstream target by phosphorylating
GSK-3beta at
serine-9 . -
pubmed
Pre - administration of
glucagon had no effect on serum
lactate and
pyruvate levels after ischemic forearm exercise test while serum
glucose elevated . -
pubmed
The
glucose clamp test confirmed
insulin resistance . -
pubmed
In these mutants , several independent insertions mapped in the
dltA gene ( involved in
D - alanine transfer in lipoteichoic acids ) and resulted in a
NucT secretion defect . -
pubmed
Metabotropic glutamate receptor type 4 is involved in autoinhibitory cascade for
glucagon secretion by alpha - cells of islet of Langerhans . -
pubmed
The
L - glutamate triggers secretion of
gamma - aminobutyric acid (
GABA ) from beta - cells , which in turn inhibits
glucagon secretion from alpha - cells through the
GABAA receptor . -
pubmed
In the present study , we tested the working hypothesis that
L - glutamate functions as an autocrine / paracrine modulator and inhibits
glucagon secretion through a
glutamate receptor(s ) on alpha - cells . -
pubmed
The addition of
L - glutamate at 1 mmol / l ;
( R , S )- phosphonophenylglycine (
PPG ) and
( S )- 3,4 - dicarboxyphenylglycine (
DCPG ) , specific agonists for
class III metabotropic glutamate receptor (
mGluR ) , at 100 micromol / l ; and
( 1S,3R,4S )- 1 - aminocyclopentane-1,3,4 - tricarboxylic acid (
ACPT - I ) at 50 micromol / l inhibited the low -
glucose - evoked
glucagon secretion by 87 , 81 , 73 , and 87 % , respectively . -
pubmed
This inhibition was dose dependent and was blocked by
( R , S )- cyclopropyl-4 - phosphonophenylglycine (
CPPG ) , a specific antagonist of
class III mGluR. -
pubmed
Agonists of other
glutamate receptors , including
kainate and
quisqualate , had little effectiveness . -
pubmed
Pertussis toxin , which uncouples
adenylate cyclase and inhibitory
G - protein , caused the recovery of both the
cAMP content and secretion of
glucagon . -
pubmed
These results indicate that alpha- and F cells express functional
mGluR4 , and its stimulation inhibits secretion of
glucagon through an inhibitory
cAMP cascade . -
pubmed
Testosterone ( 1 microM ) treatment induced a maximum increase in
beta1 - adrenergic receptor and
L - type calcium channel mRNA levels following an eight hour exposure . -
pubmed
Six hours
testosterone treatment stimulated a 300 - fold increase in
androgen receptor message abundance , and
Na /
Ca exchanger mRNA levels reached a maximum level following twenty - four hour
testosterone treatment . -
pubmed
Cluster analysis allowed identification of colinear or antiparallel expression profiles of a small set of genes involved in
post - translational regulation , and photosynthetic
carbon metabolism . -
pubmed
Upon stimulation with lipopolysaccharide /
interferon - gamma , macrophages generated a high concentration of
nitric oxide . -
pubmed
Western blot and reverse transcription
polymerase chain reaction analysis showed that
melatonin decreased the expression of
inducible nitric oxide synthase at either the protein or mRNA level . -
pubmed
Further investigation showed that
melatonin significantly attenuated the nitration of cytoplasmic
IkappaB - alpha , inhibited the degradation of
IkappaB - alpha , and blocked the translocation of
p65 / RelA into the nuclei . -
pubmed
The results suggest that
melatonin inhibits the expression of
inducible nitric oxide synthase by modulating the
nuclear factor kappaB pathway . -
pubmed
Protective mechanisms are impermeability of mucosa barrier , presence of intestinal mucus , activity of secretive immunoglobulins , some
prostaglandins and
interleukins ,
glutamine ,
somatostatin ,
cortisol and
short - chain fatty acids . -
pubmed
After dosing with the MR extracts , the thickness of the hind limbs , tibia failure load , tibia bone mineral density (
BMD ) ,
serum osteocalcin levels , tibia
calcium (
Ca ) and
phosphorus ( P ) contents were monitored with histomorphometrical changes of the tibia . -
pubmed
The macrolide
roxithromycin impairs
NADPH oxidase activation and alters translocation of its cytosolic components to the neutrophil membrane in vitro . -
pubmed
Roxithromycin alters the reconstitution of an active enzyme and impairs the translocation to the outer membrane of the cytosolic components
p47 - phox and
p67 - phox . -
pubmed
Facile preparation of amperometric
laccase biosensor with multifunction based on the matrix of
carbon nanotubes - chitosan composite . -
pubmed
The
carbon nanotubes - chitosan ( CNTs -
CS ) composite provides a suitable biosensing matrix due to its good conductivity , high stability , and good biocompatibility . -
pubmed
The system is in favor of the accessibility of substrate to the active site of
laccase , thus the affinity to substrates is improved greatly , such as
2,2 '- azino - bis -( 3 - ethylbenzthiazoline-6 - sulfonic acid ) diammonium salt (
ABTS ) ,
catechol , and
O2 with Km values of 19.86 microM , 9.43 microM , and 3.22 mM , respectively . -
pubmed
The fortuitous location of this specific stabilizing interaction in a
disulfide - bond - containing loop region of
ribonuclease A results in the localized modulation of protein dynamics that , in turn , enhances the susceptibility of the
disulfide bond to reduction leading to an alteration in the reductive unfolding behavior of the homologues . -
pubmed
RESULTS : ( 1 ) The
MVD of the
NCTD group was 4.12 + /- 1.4 , significantly lower than those of the 5 - FU group ( 15.8 + /- 5.9 ) and control group ( 17.6 + /- 3.2 ) ( both P & lt ; 0.01 ) , but not significantly different from those of the
NCTD + 5 - FU group ( 3.8 + /- 1.7 ) , ES group ( 4.5 + /- 2.1 ) , and
NCTD + ES group ( 2.9 + /- 1.5 ) ( all P & gt ; 0.05 ) . -
pubmed
( 3 ) In comparison with the control group , the mRNA expression of
VEGF and of
Ang-2 of the
tumor cells of the
NCTD group were both significantly lower and the mRNA expression of
TIMP2 was significantly higher . -
pubmed
The
PBR binds to high - affinity drug ligands and
cholesterol . -
pubmed
Many functions are associated directly or indirectly with the
PBR , including the regulation of
cholesterol transport and the synthesis of
steroid hormones ,
porphyrin transport and
heme synthesis , apoptosis , cell proliferation , anion transport , regulation of mitochondrial functions and immunomodulation . -
pubmed
By using
terminal deoxynucleotidyl transferase - mediaed
dUTP nick end labeling ( TUNEL ) , apoptotic cells were detected in 40 specimens of
breast cancer . -
pubmed
Multifrequency
EPR studies on the
Mn ( II ) centers of
oxalate decarboxylase . -
pubmed
A multifrequency cw -
EPR approach has now enabled us to show that the two
Mn ions can be distinguished on the basis of their differing fine structure parameters and to observe that
acetate and
formate bind to
Mn ( II ) in only one of the two sites . -
pubmed
The
EPR evidence is consistent with the hypothesis that this
Mn - binding site is located in the N - terminal domain , in agreement with predictions based on a recent X - ray structure of the enzyme . -
pubmed
BP554 is a
5 - HT1A agonist acting primarily on the membrane of efferent neurons by
potassium - induced hyperpolarization . -
pubmed
The
uridine nucleotide - activated
P2Y2 ,
P2Y4 and
P2Y6 receptors are widely expressed in the brain and are involved in many CNS processes , including those which malfunction in
Alzheimer ' s disease (
AD ) . -
pubmed
These results provide a basis for considering
P2Y2 receptor changes as a neurochemical substrate of
AD , and point towards
uridine nucleotide - activated
P2Y receptors as novel targets for disease - modifying
AD pharmacotherapeutic strategies . -
pubmed
Two short stretches of
amino acid sequences at the membrane proximal to the N- and C - termini (
amino acids 61 - 71 and 299 - 303 respectively ) were indispensable for Golgi localization of
RKTG , but were not required for the interaction with
Raf-1 . -
pubmed
LPA binds to selective
cell surface receptors to promote cell growth , survival , motility and differentiation . -
pubmed
Recent work identifies a secreted enzyme ,
autotaxin (
ATX ) , as the key component of an extracellular pathway for generation of
lysophosphatidic acid by
lysophospholipase D catalyzed hydrolysis of
lysophospholipid substrates . -
pubmed
Accordingly , pharmacological inhibition of
ATX may be a viable and potentially effective way to interfere with
LPA signaling in the cardiovascular system and possibly other settings such as
tumor metastasis for therapeutic benefit . -
pubmed
In this review we provide an update on recent advances in defining roles for
LPA signaling in major disease processes and discuss recent progress in understanding the regulation and function of
autotaxin focusing on strategies for the identification and initial evaluation of small molecule
autotaxin inhibitors . -
pubmed
The effects of culturing time , and
carbon and
nitrogen sources on
enzyme production were systematically investigated . -
pubmed
Stabilisation of electrochemically deposited
Prussian blue (
PB ) films on
glassy carbon (
GC ) electrodes has been investigated and an enhancement in the stability of the
PB films is reported if the electrodes are treated with
tetrabutylammonium toluene-4 - sulfonate (
TTS ) in the electrochemical activation step following the electrodeposition . -
pubmed
A multi - enzyme
PB based biosensor for
sucrose detection was made in order to demonstrate that
PB films can be coupled with an
oxidase system . -
pubmed
A tri - enzyme system , comprising
glucose oxidase ,
mutarotase and
invertase , was crosslinked with
glutaraldehyde and
bovine albumin serum on the
PB modified
glassy carbon electrode . -
pubmed
The deposited
PB operated as an electrocatalyst for electrochemical reduction of
hydrogen peroxide , the final product of the
enzyme reaction sequence . -
pubmed
The optimal concentrations of the immobilisation mixture was standardised as 8U of
glucose oxidase , 8U of
mutarotase , 16U of
invertase , 0.5 %
glutaraldehyde ( 0.025mul ) and 0.5 % BSA ( 0.025 mg ) in a final volume of 5mul applied at the electrode surface ( 0.066cm(2 ) ) . -
pubmed
An increase in the operational and storage stability of the
sucrose biosensor was also noted when the
PB modified electrodes were conditioned in
phosphate buffer containing 0.05 M
TTS during the preparation of the
PB films . -
pubmed
AIM : This paper is a report of a study to describe patients ' and nurses ' perspectives on
oxygen therapy . -
pubmed
We identified specific
amino acid propensities at the interfaces of antigen - antibody interactions in non - redundant qualified antigen - antibody complex structures from
Protein Data Bank . -
pubmed
Propensities were expressed by the frequency of each of the 20 x 20 standard
amino acid pairs that appeared at the interfaces of the complexes and were named the
antibody - specific epitope propensity ( ASEP ) index . -
pubmed
,
SLCO1B1 ) , which are potentially responsible for
AR-67 carboxylate clearance . -
pubmed
We evaluated the concentrations of
PDE5 - I ,
cAMP and
cGMP in prostate tissues and plasma , and calculated the prostate tissue - to - plasma ( T / P ) ratio . -
pubmed
RESULTS : The concentration of
udenafil in prostate tissue and plasma was 2028.6 + - 360.8 ng / g and 463.7 + -
39.1 ng / mL , respectively , and the resulting T / P ratio was 4.4 . -
pubmed
Based on a cell -
injury model made by
ozone ( 1.5 x 10(-6 ) ) exposure , the present study investigated the protective effect of
fibronectin (
Fn ) , a kind of ligand of
integrin , and it 's specific sequence
Arg - Gly - Asp ( RGD peptide ) which is a recognizable domain for
integrin on BEC . -
pubmed
3H release ,
LDH and
MDA were measured as indexes for damage . -
pubmed
RESULTS : ( 1 )
Ozone exposure induced a significant increase of
3H release and
LDH release from BEC and elevation of
MDA . -
pubmed
( 2 ) Pretreatment with
Fn or RGD before
ozone exposure markedly attenuated the release of
3H ,
LDH and
MDA , and the effects of
Fn were reversed by W7 , a
calmodulin inhibitor . -
pubmed
( 3 ) Either
Fn or RGD up - regulated activity of
catalase in BEC and promoted
GSH synthesis in BEC . -
pubmed
Previous experiments at the Rifle , Colorado Integrated Field Research Challenge (
IFRC ) site demonstrated that field - scale addition of
acetate to groundwater reduced the ambient soluble
uranium concentration . -
pubmed
Additionally ,
ABM induced DNA fragmentation , a characteristic of apoptosis and the expressions of apoptosis - related proteins , including
apoptosis - inducing factor ,
cytochrome c , and
caspase-3 , were increased . -
pubmed
: The objective of this study was to determine the
toxicity of
cisplatin - based intraperitoneal ( IP)/intravenous (
IV ) treatment using a modified version of the
IP /
IV arm of
GOG 172 . -
pubmed
BACKGROUND : Based principally on findings in three studies , the collaborative reanalysis ( CR ) , the Women 's Health Initiative ( WHI ) and the Million Women Study (
MWS ) , it is claimed that hormone replacement therapy ( HRT ) with
estrogen plus
progestogen ( E+P ) is now an established cause of
breast cancer ; the
CR and
MWS investigators claim that unopposed
estrogen therapy (
ET ) also increases the risk , but to a lesser degree than does E+P. -
pubmed
Glutathione S - transferase (
GST ) is a polymorphic supergene family involved in the detoxification of numerous toxins including
OCPs . -
pubmed
The collected samples at the time of delivery / after delivery were analyzed for
OCPs levels by gas chromatography and polymorphic analysis for
GSTM1 /
GSTT1 gene using multiplex PCR . -
pubmed
When interaction between
GSTM1 /
GSTT1 genes polymorphism -
OCPs levels and
birth weight ( gene - environment interaction ) was ascertained , a significant association was seen between beta - HCH and
GSTM1- genotype with reduction in
birth weight of 213 g. CONCLUSION : Higher levels of
OCPs in pregnant women may be considered as an important aetiological factor in ' idiopathic '
FGR . -
pubmed
Evaluation of double - disk potentiation and disk potentiation tests using
dipicolinic acid for detection of
metallo - beta - lactamase - producing pseudomonas spp . -
pubmed
A high false - positive rate is a problem with
EDTA - based
MBL detection methods . -
pubmed
aeruginosa isolates with false - negative DDPTs with MEM - DPA 250 - mug disks carried
bla(IMP-6 , ) and the high level resistance to MEM ( MIC & gt;= 512 mug / ml ) was reduced by the presence of
phenylalanine arginine beta - naphtylamide . -
pubmed
In
DPA DDPTs ,
IPM disks perform better than
MEM disks when the isolates are highly resistant to
MEM due to the overexpression of
efflux pumps . -
pubmed
In the present study , we have developed an osteoinductive coating of a porous
titanium implant using biomimetic polyelectrolyte multilayer ( PEM ) films loaded with
BMP-2 . -
pubmed
BMP-2 loaded in dried
PEM films exhibited shelf - life stability over one year . -
pubmed
Remarkably , the in vivo studies showed that the osteoinductive potential of
BMP-2 contained in
PEM - coated
Ti implants was fully preserved after air - drying of the implants and sterilization at 25 kGy . -
pubmed
We report a real - space visualization of the formation of
hydrogen bonding in
8 - hydroxyquinoline ( 8 - hq ) molecular assemblies on a
Cu ( 111 ) substrate , using noncontact atomic force microscopy (
NC -
AFM ) . -
pubmed
Intermolecular coordination between the dehydrogenated 8 - hq and
Cu adatoms was also revealed by the submolecular resolution
AFM characterization . -
pubmed
Cell organelles from
crassulacean acid metabolism ( CAM ) plants :
II . -
pubmed
After separation of cell organelles by isopycnic centrifugation ,
enzymes of the
Crassulacean acid metabolism were found in the following cell fractions :
Phosphoenolpyruvate carboxylase in the
chloroplasts ;
NAD - dependent malate dehydrogenase in the mitochondria and in the supernatant ;
NADP - dependent malate dehydrogenase and
phosphoenolpyruvate carboxykinase in the
chloroplasts ;
NADP - dependent malic enzyme in the supernatant and to a minor extent in the
chloroplasts ;
NAD - dependent malic enzyme in the supernatant and to some degree in the mitochondria ; and
pyruvate ;
orthophosphate dikinase in the
chloroplasts . -
pubmed
The activity of the
NAD - dependent malate dehydrogenase was due to three
isoenzymes separated by
( NH4 ) 2SO4 gradient solubilization . -
pubmed
It was previously demonstrated that delivery of exogenous wild - type
tumor suppressor gene
p53 via a
tumor - targeted nanocomplex ( SGT-53 ) which crosses the blood - brain barrier could sensitize highly
TMZ - resistant
GBM tumors to
TMZ . -
pubmed
Although metastable from 0 to 50 GPa , the new phase is more stable at low pressures than the other well - known
HCP and
FCC allotropes and smoothly transforms into the FCC phase under compression . -
pubmed
Burukutu consumption at 0.78 g / kg
alcohol produced no significant change in the activities of
alkaline phosphatase (
ALP ) ,
alanine aminotransferase (
ALT ) , and
aspartate aminotransferase (
AST ) . -
pubmed
Subsequently , flow cytometry was performed to investigate
indomethacin - induced apoptosis following the overexpression or knockdown of
Smac , and western blot analysis was performed to determine the expression of
Smac and the activation of
caspase-3 . -
pubmed
Western blot analysis demonstrated that
indomethacin induced apoptosis through releasing
Smac into the cytosol and activating
caspase-3 . -
pubmed
These results indicated that
Smac is essential for the apoptosis induced by
indomethacin in
esophageal cancer cells . -
pubmed
Crystal structure analysis of extrinsic
PsbP protein of
photosystem II reveals a
manganese - induced conformational change . -
pubmed
Conversely , the introduction of
bromine at
C7 of the
2,4 - dichloro [ 3,2 - d ] pyrimidine eliminated cell type - dependent differences in
cytotoxicity or cell cycle status . -
pubmed
Together , these data indicate that a substituent at
C7 can profoundly modify the cytotoxic mechanism of halogenated
thieno [ 3,2 - d ] pyrimidines in a cell type - specific manner . -
pubmed
The results demonstrated that ADSC transplantation reduced the serum levels of
alanine aminotransferase , total
bilirubin , total
cholesterol ,
triglycerides and
fatty acids , and reduced the content of
malondialdehyde in the liver homogenates . -
pubmed
If used to estimate the presence of low
rivaroxaban concentrations , POCT was superior to predictions based on normal
prothrombin time and activated partial
thromboplastin time values even if sensitive reagents were used . -
pubmed
The linear response ranges were 0.3 - 20000nM for
celecoxib , and 1.2 - 20000nM , 0.3 - 20000nM , 2.0 - 2000nM , 1.5 - 6000nM for its metabolites
carboxycelecoxib ( M2 ) ,
hydroxycelecoxib ( M3 ) ,
hydroxycelecoxib glucuronide (
M1 ) , and
carboxycelecoxib glucuronide ( M5 ) , respectively . -
pubmed
In conclusion , our results showed that our validated LC -
MS /
MS method can be successfully used for the pharmacokinetic studies of
celecoxib and that the blood cells are a very important compartment for this drug such that profiles of
celecoxib and its metabolites in whole blood will be more comprehensive and accurate representation of their profiles in vivo than the plasma . -
pubmed
Herein , a novel red - emitting
LPL phosphor
Ca3Ti2O7 :
Pr ( 3 + ) is successfully prepared by a high - temperature solid - state method . -
pubmed
Finally , a possible
LPL mechanism for
Ca3Ti2O7 :
Pr ( 3 + ) is proposed . -
pubmed
Over 73 fluorinated
proteins have been studied by
( 19 ) F NMR since the seminal studies of Hull and Sykes in 1974 . -
pubmed
With advances in cryoprobe production and fluorinated
amino acid incorporation strategies ,
protein - based
( 19 ) F NMR offers opportunities to the medicinal chemist for characterizing and ultimately discovering new small molecule protein ligands . -
pubmed
ABBREVIATIONS :
CML =
Chronic Myeloid Leukemia ,
BCR -
ABL1 = Break Cluster Region - Abelson gene , TKI =
tyrosine kinase inhibitor treatment , ACAs = additional
cytogenetic abnormalities , CCyR = complete cytogenetic response , PCyR = partial cytogenetic response , mCyR = minor cytogenetic response ,
MMR = major molecular response , HSCT = hematopoietic stem cell transplant , HLA = human leukocyte antigens ,
CP = chronic phase ,
AP = accelerated phase , BP =
blast phase , OS = overall survival , CBA = chromosome banding analysis , +8 = trisomy 8 , i(17q ) = isochromosome ( 17q ) , + Ph = second Philadelphia chromosome , -7 = monosomy 7 , -17 = monosomy 17 , +17 = trisomy 17 , -21 =
monosomy 21 , +21 =
trisomy 21 , -Y = loss of Y chromosome ,
ELN = European
LeukemiaNet , IMA600 =
Imatinib 600 mg daily ,
IMA400 =
Imatinib 400 mg daily ,
NILO600 =
Nilotinib 600 mg daily ,
DASA100 =
Dasatinib 100 mg daily ,
DASA140 =
Dasatinib 140 mg daily . -
pubmed
All - trans retinoic acid modulates
Wnt3A - induced osteogenic differentiation of mesenchymal stem cells via activating the
PI3K /
AKT /
GSK3beta signalling pathway . -
pubmed
Here , we explored whether
Wnt3A and
all - trans - retinoic acid (
ATRA ) play synergistic roles in
MSC osteogenic differentiation . -
pubmed
We found that
ATRA potentiated the
Wnt3A - induced expression of early and late osteogenic markers as well as matrix mineralization and further confirmed the phenomena using foetal limb explant culture and
MSC implantation experiments . -
pubmed
Mechanistically ,
ATRA cooperated with
Wnt3A to induce
beta - catenin translocation from cell - cell contacts into the cytosol and nucleus , thereby activating Wnt /
beta - catenin signalling . -
pubmed
Additionally ,
Wnt3A attenuated
ATRA - induced
Cyp26a1 expression , inhibiting the degradation of
ATRA into its oxidative forms . -
pubmed
beta - catenin silencing abolished the stimulatory effect of
ATRA on
Wnt3A - induced
alkaline phosphatase (
ALP ) activity and reversed its inhibitory effect on
Cyp26a1 expression . -
pubmed
Furthermore ,
ATRA and
Wnt3A synergistically promoted
AKT phosphorylation , enhancing
beta - catenin - dependent transcription through
GSK3beta inhibition or direct
beta - catenin phosphorylation at
Ser552 . -
pubmed
This event was largely abolished by
LY294002 pre - treatment , suggesting that
ATRA and
Wnt3A at least partially promote osteogenic differentiation via activating the
PI3K /
AKT /
GSK3beta signalling pathway . -
pubmed
Thus , crosstalk between the Wnt /
beta - catenin and
retinoic acid signalling pathways may be an effective therapeutic target for
bone diseases , such as
osteoporosis . -
pubmed
MAIN OUTCOMES AND MEASURES : Seventeen performance measures related to
diabetes mellitus ( including
hemoglobin A1c control , retinal eye examination ,
low - density lipoprotein cholesterol control ,
nephropathy screening , and blood pressure control ) ,
cardiovascular disease ( including
low - density lipoprotein cholesterol control , blood pressure control , and use of a beta - blocker after
myocardial infarction ) ,
cancer screening ( colorectal and breast ) , and appropriate medications ( including systemic
corticosteroids and bronchodilators for
chronic obstructive pulmonary disease [
COPD ] and disease - modifying antirheumatic drugs ) . -
pubmed
The association with adolescent fruit intake was stronger for both
estrogen and
progesterone receptor negative
cancers than
estrogen and
progesterone receptor positive
cancers ( P=0.02 for heterogeneity ) . -
pubmed
Biological variation of
glucose ,
insulin and lipids in lean ,
insulin - sensitive and
obese ,
insulin - resistant Chinese males without
diabetes . -
pubmed
The
CB1 Neutral Antagonist
AM4113 Retains the Therapeutic Efficacy of the Inverse Agonist
Rimonabant for
Nicotine Dependence and Weight Loss with Better Psychiatric Tolerability . -
pubmed
Rimonabant , a
CB1 inverse agonist found to be effective for smoking cessation , was associated with an increased risk of
anxiety and
depression . -
pubmed
In contrast to previously identified
azole - resistant isolates related to
cyp51A mutations , only one isolate carries a point mutation in
cyp51A ( F219L mutation ) among 105 independent stable
azole - resistant isolates . -
pubmed
High - performance liquid chromatography ( HPLC ) analysis verified that the decreased absorption of
itraconazole in related
Afcox10 mutants is the primary reason for
itraconazole resistance . -
pubmed
Moreover , a complementation experiment by reengineering the mutation in a parental wild - type background
strain demonstrated that both the F219L and R243Q mutations contribute to
itraconazole resistance in an
algA - independent manner . -
pubmed
These data collectively suggest that the loss of
algA results in an increased frequency of
azole - resistant isolates with a non -
cyp51A mutation . -
pubmed
Clinical and immunological efficiency of
ingavirin prevention of recurrent
herpetic stomatitis is proved by estimation of oral cavity local immunity (
SlgA , lisozyme ) , humoral immunity (
IgE and
IgG ) and cellular immunity ( RBTL with
FGA , defined T - lymphocytes ) . -
pubmed
We hypothesize that
DATS reduces
diabetic MI / R
injury by activating
SIRT1 signaling . -
pubmed
We found that
DATS treatment markedly improved left ventricular
systolic pressure and the first derivative of left ventricular pressure , reduced
myocardial infarct size as well as serum
creatine kinase and
lactate dehydrogenase activities . -
pubmed
Furthermore , the myocardial apoptosis was also suppressed by
DATS as evidenced by reduced apoptotic index and cleaved
caspase-3 expression . -
pubmed
However , these effects were abolished by
EX527 ( the inhibitor of
SIRT1 signaling , 5 mg / kg ) . -
pubmed
We further found that
DATS effectively upregulated
SIRT1 expression and its nuclear distribution . -
pubmed
Additionally ,
PERK /
eIF2alpha /
ATF4 /
CHOP - mediated
ER stress - induced apoptosis was suppressed by
DATS treatment . -
pubmed
Moreover ,
DATS significantly activated
Nrf-2 /
HO-1 antioxidant signaling pathway , thus reducing
Nox-2 / 4 expressions . -
pubmed
Taken together , these data suggest that perioperative
DATS treatment effectively ameliorates
MI / R injury in
type 1 diabetic setting by enhancing cardiac
SIRT1 signaling . -
pubmed
Chronic
dexamethasone treatment results in hippocampal neurons
injury due to activate
NLRP1 inflammasome in vitro . -
pubmed
The present results showed that chronic
DEX exposure significantly increased
LDH release and apoptosis , decreased
MAP2 and
GR expression in hippocampal neurons . -
pubmed
DEX ( 5muMu ) exposure for 3d significantly increased the expression of
NLRP-1 ,
ASC ,
caspase-1 and
IL-1beta in the hippocampal neurons and the release of
IL-1beta and
IL-18 in the supernatants . -
pubmed
Moreover ,
DEX ( 1 , 5muMu ) treatment for 3d significantly increased the expression of
NF - kappaB in hippocampal neurons . -
pubmed
The
GR antagonist ,
mifepristone (
RU486 ) , had protective effects on chronic
DEX induced
hippocampal neurons injury and
NLRP1 inflammasome activation . -
pubmed
The
carbapenem resistance mechanisms such as carbapenemase genes ,
efflux pump hyperexpression ,
AmpC overproduction , and
OprD gene downregulation were determined by phenotypic and molecular methods . -
pubmed
Eighteen
metallo - beta - lactamase (
MBL ) producer isolates were found to be sensitive to
amikacin . -
pubmed
The clonal distribution of the
strains was related to
carbapenem resistance mechanisms ( most of
MBL producers belong to the same clones ) and the same hospital wards where the isolates were collected . -
pubmed
Amikacin was considered a very efficient antibiotic to treatment of
MBL - producing CRPA isolates . -
pubmed
Our results showed that
OprD downregulation and
IMP - type MBL are the main
carbapenem resistance mechanisms in
CRPA isolates from northwest of Iran . -
pubmed
Functional Consequences of Intracellular
Proline Levels Manipulation Affecting
PRODH /
POX - Dependent Pro - Apoptotic Pathways in a Novel in Vitro Cell Culture Model . -
pubmed
BACKGROUND / AIMS : The effect of impaired intracellular
proline availability for
proline dehydrogenase /
proline oxidase (
PRODH /
POX)-dependent apoptosis was studied . -
pubmed
We have used inhibitor of
proline utilization in
collagen biosynthesis ,
2 - metoxyestradiol (
MOE ) , inhibitor of
prolidase that generate
proline ,
rapamycin (
Rap ) and
glycyl - proline (
GlyPro ) , substrate for
prolidase . -
pubmed
The optimized LC -
MS /
MS method described here has been utilized extensively for metabolomics analysis of polar
metabolites . -
pubmed
T-614 Promotes Osteoblastic Cell Differentiation by Increasing
Dlx5 Expression and Regulating the Activation of
p38 and
NF - kappaB. -
pubmed
The contents and activity of
alkaline phosphatase , levels of
collagen type I and
bone gla protein , and
calcium nodule formation were increased significantly after
T-614 treated . -
pubmed
The results showed that
T-614 promotes osteoblastic differentiation by increasing the expression of
Osterix and
Dlx5 and increasing the activation of
P38 . -
pubmed
T-614 could advance the ectopic expression of
NF - kappaB to suppress
inflammation , which indirectly inhibits the damage of the osteoblasts . -
pubmed
ArMR displayed the optimum catalytic activity at 50 C , pH 7.8 in
Tris - HCl buffer . -
pubmed
KM parameter of
ArMR towards
( R )- and ( S )- mandelic acid was 1.44 and 0.81 mmol / L , respectively ; the corresponding kcat value was 410 s-1 and 218 s-1 . -
pubmed
In addition , KM of
ArMR towards
( R )- and ( S )- 2 - chloro mandelic acid was 6.48 and 6.37 mmol / L , and the corresponding kcat value 0.22 s-1 and 0.23 s-1 , respectively . -
pubmed
Meanwhile ,
Mg2 + and
Mn2 + could activate the
enzyme whereas
Zn2 + inactivated the
enzyme completely . -
pubmed
The results revealed that
LBP protected the proliferative ability of trophoblast cells injured with
H2O2 in a dose - dependent manner . -
pubmed
LBP inhibited the oxidative stress induced by
H2O2 , by reducing ROS and
LDH levels and increasing
SOD activity . -
pubmed
Additionally ,
LBP decreased
MMP disruption and cell apoptosis induced by
H2O2 , by increasing the mRNA and protein expression of
survivin ,
HIF1 - alpha and
Bcl-2 and decreasing
Bax expression . -
pubmed
Presynaptic
Ca2 + accumulation in the terminal was also enhanced in
DRP1 - preKO synapses . -
pubmed
Synaptic transmission defects could be partially rescued with
EGTA -
AM , indicating close coupling of
Ca2 + channels to SV distance normally found in mature terminals may be compromised by
DRP1 - preKO . -
pubmed
The characterization studies of these derivatives were carried by using different spectroscopic techniques (
1H NMR ,
13C NMR and
MS ) and elemental analyses . -
pubmed
Vemurafenib , a selective
BRAF kinase inhibitor , has been found to induce several cutaneous adverse effects , ranging from a
keratosis pilaris - like reaction to
squamous cell carcinoma . -
pubmed
Abbreviations : BM = bone
metastases ;
COX = multivariate analyses ;
DM = distant
metastases ; DSM =
disease - specific mortality ;
DSS =
disease - specific survival ;
DTC =
differentiated thyroid carcinoma ;
ETE = extrathyroidal extension ;
LNM = lymph node
metastases ;
OM = overall mortality ; OS = overall survival ; PTCFV =
papillary thyroid carcinoma ;
RAI = radioactive
iodine ; SM = spinal
metastases ;
SRE = skeletal - related event ; txWBS = whole - body scan after
RAI therapy . -
pubmed
Hepcidin is a main regulator of
iron metabolism , of which abnormal expression affects intestinal absorption and reticuloendothelial sequestration of
iron by interacting with
ferroportin . -
pubmed
Alborixin clears
amyloid - beta by inducing autophagy through
PTEN - mediated inhibition of the
AKT pathway . -
pubmed
We found that autophagy induced by
alborixin substantially cleared
Abeta in microglia and primary neuronal cells . -
pubmed
Autophagy induced by
alborixin was associated with inhibition of the
phosphoinositide 3 - kinase (
PI3K)-
AKT pathway . -
pubmed
A knock down of
PTEN and consistent , constitutive activation of
AKT inhibited
alborixin - induced autophagy and consequent clearance of
Abeta . -
pubmed
Abbreviations :
Abeta : amyloid beta ;
ALB : alborixin ; ATG : autophagy - related ;
BECN1 :
beclin 1 ;
DAPI :
4 , 6 - diamidino-2 - phenylindole ;
DCFH - DA :
2,7 - dichlorodihydrofluorescein diacetate ;
fAbeta : fibrillary form of
amyloid beta ;
GFAP :
glial fibrillary acidic protein ;
MAP1LC3B /
LC3B :
microtubule - associated protein 1 light chain 3 beta ;
MAP2 :
microtubule - associated protein 2 ;
MTOR :
mechanistic target of rapamycin kinase ;
PTEN :
phosphatase and tensin homolog ; ROS : reactive
oxygen species ;
SQSTM1 :
sequestosome 1 ;
TMRE :
tetramethylrhodamine ,
ethyl ester . -
pubmed
BACKGROUND : Previous research demonstrates increased utilization of high - intensity statins , but unchanged
low - density lipoprotein cholesterol (
LDL - C ) levels , immediately after the 2013 American College of Cardiology (
ACC ) and the American Heart Association ( AHA ) guideline release . -
pubmed
Atomic force microscopy (
AFM ) normal force measurements provide insight into the architecture of the HA /
LUB composite layer and implicate a strong contribution of hydrophobic interactions in the binding of
LUB end - domains directly to HA chains . -
pubmed
These
AFM force measurements indicate that the adhesion of
LUB to HA is strong and indicate that the hydrophobic coupling of LUB to HA shields the hydrophobic domains in these molecules from interactions with other proteins or molecules . -
pubmed
Prmt7 promotes myoblast differentiation via methylation of
p38MAPK on
arginine residue 70 . -
pubmed
The mechanistic study reveals that
Prmt7 methylates
p38MAPKalpha at the
arginine residue 70 , thereby promoting its activation which in turn enhances
MyoD activities . -
pubmed
The
arginine residue 70 to alanine mutation in
p38MAPKalpha impedes
MyoD /
E47 heterodimerization and the recruitment of
Prmt7 ,
MyoD and
Baf60c to the
Myogenin promoter resulting in blunted
Myogenin expression . -
pubmed
In conclusion ,
Prmt7 promotes
MyoD - mediated myoblast differentiation through methylation of
p38MAPKalpha at
arginine residue 70 . -
pubmed
PANF - ATL was characterized by elemental analysis , FTIR ,
TGA , SEM and XPS . -
pubmed
The transfer factor (
TF ) and the total bioaccumulation amount ( TBA ) of
uranium were used to describe remediation efficiencies in this paper . -
pubmed
Results indicated that sperm motility was significantly reduced from 1 day following
TiNP injection ( in both dose ) , and this reduction persisted up to 9 days
post -
TiNP injection ( 10 mg / kg injection group ) . -
pubmed
The LC sensor was fabricated by immobilizing 3 microg mL-1
BSA solution on
dimethyloctadecyl -[ 3 -( trimethoxysilyl ) propyl ] ammonium chloride (
DMOAP)-coated glass slides . -
pubmed
Three - point bending tests showed higher mechanical properties for
PUF -
DCPD ( 80 % ) than
MUF -
ENB ( 40 % ) compared to the neat
epoxy systems . -
pubmed
Serum
25 - hydroxyvitamin D (
25 ( OH ) D ) levels were determined by radioimmunoassay and liver and adipose tissue
cholecalciferol (
VD3 ) and
25 - hydrocholecalciferol (
25 ( OH ) D3 ) levels were measured by LC -
MS /
MS . -
pubmed
There was no difference in mRNA levels of liver
25 - hydroxylases by both dietary fat amount and
vitamin D content . -
pubmed
Although the total
cholesterol values were normal , the
very - low - density lipoprotein of
cholesterol level was high ( 49 + - 7 mg / dL ) . -
pubmed
The mean values of total
triglyceride ,
very - low - density lipoprotein of both
triglyceride and
cholesterol decreased significantly after the treatment period ( 319 + - 29 , 245 + - 18 , and 27 + - 2 mg / dL , respectively , P < 0.05 ) . -
pubmed
The addition of
Ag+ triggered the self - assembly of branched PNAs through the formation of
C - Ag +- C metallo base pairing of the three PNA
C4 arms leading to non - covalent dendrimers , whose architecture is directed by the
C4 ( R / S )-stereocenter of core
4 - aminoproline . -
pubmed
Post - ban samples were asked about awareness of the ban and access to
menthol cigarettes . -
pubmed
However , odds were lower
post - ban for reporting
menthol as the usual cigarette ( OR=0.80 , 95 % CI 0.72 to 0.90 ) , and for smoking only
menthol cigarettes ( OR=0.19 , 95 % CI 0.18 to 0.19 ) . -
pubmed
Moreover , Cm3639 and
Cm3769 can convert the
GlcNAc dimer and colloidal chitin ( CC ) into
GlcNAc , which showed that they also possess
NAGase activity . -
pubmed
In addition ,
NAGase Cm3245 possesses a very high exo - acting activity of hydrolyzing
GlcNAc dimer . -
pubmed
These results suggest that
chitinases and
NAGase from
SYBC - H1 both play important roles in conversion of
N - acetyl chitooligosaccharides to
GlcNAc , resulting in the accumulation of the final product
GlcNAc . -
pubmed
The intravenous injection of
glucose as a " therapeutic test " in
comatose diabetics is reviewed in the light of evidence that it may evoke a paradoxical rise in plasma -
potassium in the
insulin - deprived
diabetic . -
pubmed
The plasma -
potassium rose promptly after a drink of 50 g
glucose in 8
insulin - dependent
diabetics who had not had
insulin for approximately 14 - 26 hours . -
pubmed
Initially , the microsomal mixed function
oxidase system was assumed to be capable of oxidizing
ethanol in a mechanism that did not involve either
alcohol dehydrogenase or
catalase . -
pubmed
Later evidence suggested that the oxidative
enzyme was , in fact ,
catalase , a contaminant of microsomal preparations and that the mixed function
oxidase system merely furnished
hydrogen peroxide to the reaction . -
pubmed
The
actin content of fibroblasts may be low or the
amino acid sequences of muscle and
fibroblast actin may differ in the N - terminal region . -
pubmed
Lectin affinity chromatography of proteins bearing
O - linked oligosaccharides : application of jacalin -
agarose . -
pubmed
These proteins were eluted from columns of
jacalin - agarose specifically by
alpha - galactopyranosides such as
melibiose and
alpha - methylgalactopyranoside but not by
lactose or other
sugars . -
pubmed
Treatment of
asialofetuin with
endo -- alpha -- N -- acetylgalactosaminidase eliminated its affinity for the
lectin column , and other proteins known to contain only
N - linked oligosaccharides such as
ovalbumin ,
transferrin , and
alpha 1 - acid glycoprotein were not retained by the
lectin . -
pubmed
Binding of
proteins with
O - linked oligosaccharides to the
lectin column did not require divalent cations and was affected little by changes in pH and ionic strength over a wide range . -
pubmed
Virtually all of the glycosidically linked
oligosaccharides of
fetuin ,
chorionic gonadotropin , and
plasminogen are known to be sialated . -
pubmed
Thus , binding of these
glycoproteins to
jacalin , which is known to have affinity for the core
disaccharide ,
1 - beta - galactopyranosyl-3 -( alpha-2 - acetamido-2 - deoxygalactopyranoside ) , in
O - linked oligosaccharides of these proteins , was not prevented by the presence of
sialic acids . -
pubmed
Affinity of
oligosaccharides for
jacalin did appear to be reduced by occurrence of
sialic acids as it was found that higher concentrations of
melibiose were required to elute
asialofetuin than
fetuin from jacalin - agarose . -
pubmed
Results of the present study indicate that affinity chromatography using this
lectin is a widely applicable technique for identifying and purifying proteins bearing
O - linked oligosaccharides . -
pubmed
Several polypeptide mediators capable of stimulating fibroblasts replication or excessive
collagen deposition have been implicated in this , but the precise role of these in
bleomycin - induced
fibrosis is yet to be demonstrated . -
pubmed
The beta - turn formed by the
amino acid residues 20 - 23 of the B - chain of
insulin has been implicated as an important structural feature of the molecule . -
pubmed
In two analogues , we have substituted
alpha - aminoisobutyric acid (
Aib ) for the
Glu residue normally present in position B21 or for the
Arg residue normally present in position
B22 ; in a third compound , we have replaced the
Glu residue with its D - isomer . -
pubmed
D -[ U-14C ] Glucose tracer exchange , measured under voltage -
clamp condition at
Na+/D -
glucose equilibrium , revealed that addition of
calcitriol to the culture medium approximately doubled the activity of the
Na +/ D - glucose transporter in the brush - border membrane . -
pubmed
Further , a model including age , smoking habits ,
fibrinogen , viscosity , and white blood cell count predicts
IHD as well as one in which the three hemostatic / rheological variables are replaced by total
cholesterol ,
diastolic pressure , and
body mass index . -
pubmed
Because
Ca2 + has been implicated as a regulator of mitogenic processes in a number of tissues , we investigated whether
GH affects the transmembrane movement of
Ca2 + through voltage - activated channels of cardiac myocytes . -
pubmed
The results suggest that a
tumor - derived growth factor , most likely
GH , can cause a specific enhancement of T - type
Ca2 + current in atrial myocytes . -
pubmed
Neither the plasma
insulin response to meals nor
insulin - stimulated
glucose uptake improved with
acarbose therapy , consistent with the view that
acarbose improves glycemic control by delaying
glucose absorption . -
pubmed
Ga - labeling of
immunoglobulin G with high specific radioactivity . -
pubmed
To obtain
radiogallium labeled
immunoglobulin G with a high specific radioactivity for in vitro use , a
67Ga source was purified by extraction from
67Ga - gallium citrate with
butyl acetate , and a
67Ga - labeling solution was produced . -
pubmed
The
adenosine - stimulated increase in vascularity was blocked with 110 micrograms of
methyl - isobutyl - xanthine injected daily into the
albumin . -
pubmed
Compounds I and
II were also formed from the reaction of 1.0 microM
20 - OH - LTB4 , PMN microsomes , and 100 microM
NADPH ; the identity of compound
II was confirmed as
20 - COOH - LTB4 by gas chromatography - mass spectrometry . -
pubmed
Addition of immune
IgG to
cytochrome P-450 reductase or 1.0 mM
SKF-525A completely inhibited the formation of
20 - CHO - LTB4 from
20 - OH - LTB4 , indicating that the reaction was catalyzed by a
cytochrome P-450 . -
pubmed
LTB5 ( 3.0 microM ) , a known substrate for
cytochrome P-450LTB and a competitive inhibitor of LTB4 omega - oxidation , completely inhibited the omega - oxidation of 1.5 microM
20 - OH - LTB4 to
20 - CHO - LTB4 , indicating that the
cytochrome P-450 was P-450LTB . -
pubmed
Conversion of 1.0 microM
20 - CHO - LTB4 to
20 - COOH - LTB4 by PMN microsomes was also dependent on
NADPH and inhibited by
antibody to
cytochrome P-450 reductase , 1.0 mM
SKF-525A , or 5.0 microM
LTB5 , indicating that this reaction was also catalyzed by
cytochrome P-450LTB . -
pubmed
These results identify the novel metabolite
20 - CHO - LTB4 and indicate that
cytochrome P-450LTB catalyzes three sequential omega - oxidations of
LTB4 leading to the formation of
20 - COOH - LTB4 via
20 - OH - LTB4 and
20 - CHO - LTB4 intermediates . -
pubmed
By comparing inactivating effects of
diethyldithiocarbamic acid mixed
disulfides with
thiols of increasing chain length evidence is provided that steric hindrance is the reason for lack of inhibition of the mitochondrial
enzyme by
disulfiram in vitro . -
pubmed
52 , 187 - 222 ] the results also suggest a mechanism by which
aldehyde dehydrogenase is inhibited by
disulfiram and
diethyldithiocarbamic acid in vivo . -
pubmed
Lymphocyte functions , such as
PHA response , surface marker and
serum IAP , before and after
NCS injections were the same . -
pubmed
Epidermal growth factor receptor expression during morphological differentiation of
pheochromocytoma cells , induced by
nerve growth factor or
dibutyryl cyclic AMP . -
pubmed
In addition , exposure of the cells to
dbcAMP caused a twofold increase of
EGF - receptor phosphotyrosine kinase activity . -
pubmed
It is shown that
PRO induces mutants by means of " mutation - mitotic segregation " mechanism , while
HAP induces mutants through novel mechanism " both allele mutation " . -
pubmed
We used
phosphate buffer with
Triton X-100 for extracting
apoB on
DBS . -
pubmed
While there was little effect of most of the native preparations tested , some batches showed significant release of
histamine or
lysosomal enzymes after heating to 70 degrees C.
HSA preparations produced by heat
ethanol fractionation never induced significant release reactions . -
pubmed
Oxidation of
glutathione by the
myeloperoxidase system . -
pubmed
Oxidation of
glutathione (
GSH ) by the
myeloperoxidase (
MPO ) system was studied . -
pubmed
The combination of
MPO ,
H2O2 , and a
halide ion oxidized
GSH . -
pubmed
The
MPO - mediated oxidation of
GSH required the simultaneous presence of
MPO ,
H2O2 , and a
halide ion . -
pubmed
The oxidative product was shown to be
GSSG , since it could be reduced back to
GSH by
glutathione reductase and
NADPH . -
pubmed
The
MPO - mediated oxidation of
GSH may be one mechanism by which this system damages microorganisms . -
pubmed
Psoralens were placed specifically near the
BamHI site , in the
tetracycline - resistance gene of pBR322 , using a
sulfhydryl - containing
psoralen derivative . -
pubmed
Morphine analgesia potentiated but tolerance not affected by active immunization against
cholecystokinin . -
pubmed
This process goes from immature ( To ) to semi - mature (
T1 ) and mature (
T2 ) lymphocytes , these latter mostly characterized by the absence of
TdT and by the property to form E - rosettes with
SRBC and respond to
mitogens . -
pubmed
These mitochondria have a large capacity to flux
pyruvate through the
pyruvate carboxylase reaction causing an accumulation of
malate ,
citrate and
phosphoenolpyruvate . -
pubmed
As much as 70 % of the
pyruvate utilized during state 3 respiration can flux through the
pyruvate carboxylase reaction . -
pubmed
The reactivity of
Ab1 and
Ab3 with two
amantadine - resistant virus mutants indicates that
IIF4 epitope ( and its related region recognized by
Ab3 ) becomes accessible in consequence of destabilization of trimeric arrangement of
HA and it also correlates with expulsion of N - terminus of
HA2 . -
pubmed
The routinely used
immunoenzyme peroxidase ( PO ) labelling has been replaced by
alkaline phosphatase ( AP ) using new
fuchsin as substrate . -
pubmed
The
enzyme was also active against
alpha - fructosyl lysine and
borohydride - reduced
Amadori product , weakly active with ribated and glycated
polylysine , and inactive with reducing
sugars ,
amino acids , and glycated proteins . -
pubmed
The reaction products were characterized by 1H NMR , 13C NMR , and
GC /
MS and found to correspond to
1 - deoxy-1 - aminofructose , i.e. -
pubmed
In contrast , however , the new
enzyme acts on the
alkylamine bond rather than the
ketoamine bond of the Amadori product . -
pubmed
Postexercise elevations in serum
cortisol and
prolactin were seen only in the male subjects . -
pubmed
For serum
testosterone , neither resting nor higher
post - exercise concentrations were different at any point of the training periods for either sex . -
pubmed
Relationship between plasma
triglycerides and
apolipoprotein CII in infants during the first year of life . -
pubmed
Plasma
triglyceride and
apolipoprotein CII (
apo CII ) levels were measured in normal full - term infants and low - birth - weight infants at birth ( from umbilical cord blood at 0 weeks ) , as well as at 2 , 3 , 4 , 8 , 24 , and 48 weeks of age , and the relationship between plasma
triglyceride and
apo CII levels was studied . -
pubmed
In both full - term and low - birth - weight infants , the plasma
triglyceride and
apoprotein CII levels were very low at birth , but they increased rapidly thereafter in the full - term infants and somewhat more slowly in the low - birth - weight infants . -
pubmed
Both
triglyceride and
apoprotein CII levels became stable from 3 weeks of age in full - term infants and from 8 weeks in low - birth - weight infants . -
pubmed
Characterization of
HIV1 - PAR , a macrophage - tropic
strain : cell tropism , virus / cell entry and
nucleotide sequence of the
envelope glycoprotein . -
pubmed
Five
CD4 - binding domains , the
gp120 /
gp41 cleavage site , the putative
gp41 fusion domain and 21 out of the 22
cysteine residues were conserved in both isolates . -
pubmed
Competition binding studies using
AH 6809 (
EP1 antagonist ) ,
butaprost (
EP2 agonist ) , M/+
B 28,767 (
EP3 agonist ) , and
AH 23848B (
EP4 antagonist ) suggested that the retinal vessels of the newborn contained approximately equal number of
EP1 and
EP2 receptor subtypes whereas the main receptor subtype in the adult vessels was
EP1 . -
pubmed
PGE2 ,
17 - phenyl trinor PGE2 (
EP1 agonist ) and
PGF2 alpha caused a 2.5 to 3 - fold greater increase in
inositol 1,4,5 - triphosphate (
IP3 ) formation in adult than in newborn preparations . -
pubmed
Two of 5 transformants obtained as
bialaphos - resistant colonies showed two to twenty times higher specific activity of
GUS than the recipient . -
pubmed
Based on this finding , scanning mutagenesis was used to find a single -
cysteine mutant of the pore - forming protein
staphylococcal alpha - hemolysin (
alpha HL ) suitable for caging . -
pubmed
A
post - capillary reactor is constructed to allow on - line addition of fragment B ( of protein A)-
fluorescein to form affinity complexes with separated
IgG subclasses . -
pubmed
Ethanol disrupts signal transduction mediated by a variety of
G - protein coupled receptors . -
pubmed
We examined the effects of
ethanol on
arachidonic acid release mediated by
muscarinic acetylcholine receptors . -
pubmed
Dose response curves suggest that
ethanol acts as a noncompetitive inhibitor of
muscarinic receptor - induced [
3H]AA release insofar as maximal
[ 3H ] AA release was depressed in the presence of
ethanol with no apparent change in the EC50 for stimulation by
carbamylcholine . -
pubmed
These results indicate that
ethanol acutely inhibits
muscarinic receptor signaling through the
arachidonic acid pathway in a noncompetitive manner , but chronically enhances muscarinic signaling through the same pathway . -
pubmed
The
ATPase activity was inhibited by
NO3 - and
SCN- which are the inhibitors of
V - type ATPase , but not by
azide and
vanadate , inhibitors of
F - type ATPase and
P - type ATPase , respectively . -
pubmed
PCR - based analysis of the freshly harvested
tumor from patient PM2 - B2 revealed the presence of
message for the
melanoma - associated gene products MAGE-1 and
MAGE-3 , but not for
tyrosinase or
MART-1 /
MELAN - A. -
pubmed
8 M
KCl , which contained
transcription factor IID (
TFIID ) , overcame the inhibition . -
pubmed
BACKGROUND : Deep hypothermic circulatory arrest ( DHCA ) has been shown to cause impairment in recovery of cerebral blood flow (
CBF ) and cerebral metabolism ( CMRO2 ) proportional to the duration of the
DHCA period . -
pubmed
This effect on CMRO2 may be a marker for
brain injury , because CMRO2 recovers normally after cardiopulmonary bypass (
CPB ) when
DHCA is not used . -
pubmed
The aim of this study was to investigate the effects of intermittent perfusion during
DHCA on the recovery of CMRO2 after
CPB and to correlate these findings with electron microscopy ( EM ) of the cerebral microcirculatory bed . -
pubmed
RESULTS : UI -
DHCA resulted in significant impairment to recovery of CMRO2 after
CPB ( p & lt ; 0.05 ) . -
pubmed
Thus , the co - existence of
NADPH - d and
AChE occurred in the location of interneurons . -
pubmed
Radiolabeled derivatives of
WAY-100635 have been shown to be important for imaging in vivo because of their antagonist properties and their specificity for the
5 - hydroxytryptamine ( 1A ) ( 5 - HT ( 1A ) ) receptor . -
pubmed
He was administered
benzinpenicilline ,
chloramphenicol - succinate ,
lanatoside ,
human albumins ,
dexamethasone .... -
pubmed
Statins ,
3 - hydroxy-3 - methylglutaryl coenzyme A ( HMG - CoA ) reductase inhibitors , are a class of drugs with a potent lipid - lowering effect that have been shown to reduce LDL
cholesterol by 35 - 45 % at therapeutic doses . -
pubmed
At present , the primary target of statins is LDL
cholesterol reduction , but with additional effects on the inflammatory cell of the plaque with a reduction in macrophages and secretion of collagenolytic metalloproteases and reinforcing the fibrous skeleton of the plaque by increasing the content of interstitial
collagen . -
pubmed
The increased serum
adrenocorticotropic hormone ,
corticosterone and
serotonin levels observed following
melatonin and
p - chloroamphetamine treatment suggest that this combination may have adverse peripheral effects . -
pubmed
Nitric oxide (
NO ) regulates
cyclo - oxygenase (
COX ) activity in various cell systems and reports conflict in regard to its stimulatory versus inhibitory role . -
pubmed
The
COX-2 induction was dependent on a de novo synthesis since
cycloheximide , an inhibitor of protein synthesis , blocked the enzyme expression . -
pubmed
The increase in
COX-2 expression was not accompanied by a corresponding change in
prostaglandin (
PG ) production . -
pubmed
However , the
COX activity was partially recovered when immunoprecipitated
COX-2 was incubated with
arachidonic acid and
haematin . -
pubmed
Peroxynitrite , a highly reactive
nitrogen molecule derived from the interaction of
NO and
superoxide anion , significantly increased
COX-2 expression . -
pubmed
Under these conditions and within the limit of detection of the antibody , selective antibody for
nitrotyrosine failed to detect nitrated
COX-2 in immunoprecipitated
COX-2 when cells where incubated with
SIN-1 or SIN-1+
IL-1alpha . -
pubmed
Ro 31 - 8220 , a specific inhibitor of
protein kinase ( PK ) C , blocked the induction of
COX-2 . -
pubmed
Also ,
SB203580 , the selective inhibitor of
p38 MAP kinase , strongly blocked the induction of
COX-2 by
SIN-1 in the presence or absence of
IL-1alpha , whereas the
MEK-1 inhibitor ,
PD 98059 , affected it to a lesser extent . -
pubmed
These data demonstrate that
SIN-1 induces
COX-2 in HUVEC in the absence of PG formation and suggest a complex regulation of
COX-2 expression and PG formation by
NO in endothelial cells . -
pubmed
To block the
Fas -
Fas ligand interaction , an anti -
Fas ligand antibody (
FasL ) was injected with
ADR 7 , 8 , and 9 wk after first administration of
ADR . -
pubmed
Fas antigen was overexpressed in the hearts of
ADR and
ADR+
FasL groups , however , an expression of
Fas antigen in the
pirarubicin group was similar to the expression of
Fas antigen in the control group . -
pubmed
Synthesis of
calcineurin - resistant derivatives of
FK506 and selection of compensatory receptors . -
pubmed
We used
olefin metathesis to synthesize
C40 derivatives of
FK506 and measured their ability , when complexed to
FKBP12 , to inhibit
calcineurin ' s phosphatase activity . -
pubmed
Using chemical genetic selection , we identified compensatory mutant
CABs that respond to a
calcineurin - resistant
FK506 derivative at concentrations well below the response threshold for
CABs containing only wild - type
calcineurin sequence . -
pubmed
The
cytotoxicity of 5 against a panel of
tumor cell lines , including several with multidrug resistance , and its effect on
tubulin polymerization were comparable to
epothilone D ( 4 ) . -
pubmed
RESULTS :
NOS 3 immunoreactivity and amount of protein ( western blotting ) were significantly decreased in
CCl ( 4 ) cirrhotic livers . -
pubmed
No changes were observed in systemic haemodynamics , in liver tests or urinary
nitrates , or in
NOS 3 expression in lung or kidney , indicating a highly selective transfer . -
pubmed
These data indicate a major haemodynamic role of intrahepatic
NOS 3 in the pathogenesis of
portal hypertension in
CCl ( 4 ) cirrhosis . -
pubmed
The prevalence of mutations was lower among those receiving other
nucleoside reverse transcriptase inhibitors and
protease inhibitors . -
pubmed
delivery of low doses of
8 - bromo - cGMP ( 0.1 - 0.25 micromol ) reduced nociceptive behavior and
formalin - induced upregulation of
PKG - I in the spinal cord . -
pubmed
To explain these dose - dependent contrary effects we assessed the potential involvement of various
cGMP targets :
protein kinase G ,
cyclic nucleotide gated cation channels (
CNGs ) ,
phosphodiesterases (
PDE2 and
PDE3 ) and
AMPA - receptors . -
pubmed
The
PKG inhibitor ,
Rp-8 - bromo - cGMPS did not antagonize the antinociceptive effects of
8 - bromo - cGMP but caused antinociception itself . -
pubmed
Inhibitors of
CNGs ,
PDE2 and
PDE3 had no effect on
formalin evoked nociceptive behavior . -
pubmed
Since
AMPA receptor currents were found to be reduced by
8 - bromo - cGMP in vitro a direct or indirect reduction of
AMPA receptor currents might possibly contribute to the antinociceptive effects of
8 - bromo - cGMP . -
pubmed
On the other hand ,
8 - bromo - cGMP evoked antinociception appears to be largely independent of
PKG - I ,
CNGs ,
PDE2 and
PDE3 . -
pubmed
The antinociceptive effects of the
PKG inhibitor suggest that a strong
PKG activation may be responsible for ' high dose '
8 - bromo - cGMP evoked
hyperalgesia . -
pubmed
BACKGROUND AND
AIM OF THE STUDY : Calcification of bioprosthetic heart valves fabricated from
glutaraldehyde ( GA)-pretreated heterograft tissue is frequently responsible for the clinical failure of these devices . -
pubmed
Ethanol pretreatment of bioprosthetic heart valves was shown to inhibit
cuspal calcification due to multiple mechanisms , including alterations of
collagen structure and lipid extraction . -
pubmed
AlCl3 pretreatment of bioprostheses to prevent calcification was also investigated ; this alters
elastin structure , inhibits
alkaline phosphatase , and complexes with
phosphoesters , thereby inhibiting aortic wall mineralization . -
pubmed
All tested compounds but one (
Ro 15 - 1570 ) revealed a dose - dependent inhibition of
RNase P activity , indicating that they may have a direct effect on
tRNA biogenesis . -
pubmed
Activin A ,
hypoxanthine (
Hx ) ,
xanthine ( Xa ) plasma levels and absolute nucleated red blood cell ( NRBC ) count were measured . -
pubmed
PHOSVITIN , THE
IRON CARRIER OF EGG YOLK . -
pubmed
Estrogen , another crucial factor in
breast cancer , stimulates
VEGF ; and an ERE in the
VEGF gene has been defined . -
pubmed
We show , using intratumoral microdialysis in vivo , that E(2 ) treatment increased
tumor extracellular levels of
VEGF in an
estrogen - dependent
breast cancer model . -
pubmed
Arp residues expected to interact with
nucleotide and with the first and second
actin subunits in a daughter filament are conserved and similar to
actin . -
pubmed
This provides strong evidence that
c - kit receptor mediated
tyrosine kinase involvement in the pathogenesis of
phyllodes tumors , and the therapeutic agent ,
STI571 ,
Glivec , may be a potentially useful drug for its management . -
pubmed
PURPOSE : We report penile length (
PL ) responses to
testosterone enanthate ( TE ) therapy for
micropenis , and the relevance of the V89L polymorphism of
SRD5A2 encoding the
5alpha - reductase type 2 and CAG repeat length polymorphism of
AR encoding the
androgen receptor . -
pubmed
This method is tested on the
F ( 2P )+ H2O-->
HF+
OH ( 2Pi ) reaction , which otherwise proves challenging to describe with traditional
SA - MCSCF methods due to ( i ) different asymptotic degeneracies of reactant ( threefold ) and product ( twofold ) channels , and ( ii ) presence of low - lying charge transfer configurations near the transition state region . -
pubmed
In this study , the effects of SJW on a
benzodiazepine hypnotic ,
quazepam , which is metabolized by
CYP3A4 , were examined . -
pubmed
The urinary ratio of
6beta - hydroxycortisol to
cortisol , which reflects
CYP3A4 activity , also increased after dosing with SJW ( ratio ; 2.1 ( 95%CI 0.85 - 3.4 ) ) . -
pubmed
CONCLUSIONS : These results suggest that SJW decreases plasma
quazepam concentrations , probably by enhancing
CYP3A4 activity , but does not influence the pharmacodynamic effects of the drug . -
pubmed
BACKGROUND AND OBJECTIVES :
Arginine 315 in
factor VII (
FVII ) belongs to a solvent - exposed loop involved in direct interaction with the co - factor (
tissue factor ,
TF ) , in transmission of
TF - induced effects and potentially in
FVIIa inactivation . -
pubmed
DESIGN AND METHODS : We characterized a mild
coagulation FVII deficiency associated with reduced
FVII activity ( 26 % ) and
antigen ( 67 % ) . -
pubmed
For
PWWF / ADWF ratios in the upper range ( f(q ) approximately 2.0 ) , aerobic mass fractions in the lower range ( f(maer ) & lt ; 0.60 ) , and high ( usually raw ) wastewater strengths , the indicated mode of operation of
MBR BNR systems is as extended aeration WWTPs . -
pubmed
MMP-9 levels positively correlated with
TIMP-1 and
lactate dehydrogenase levels , and negatively with pH and
glucose levels in
pleural effusions . -
pubmed
Short - term
progestin treatments prevent estrous induction by a
GnRH agonist implant in anestrous bitches . -
pubmed
Abstract
Raloxifene and low - dose
simvastatin can each reduce
low - density lipoprotein ( LDL )
cholesterol without affecting
high - density lipoprotein ( HDL )
cholesterol and
triglycerides . -
pubmed
The objective of this double - blind , 12 - week study is to determine whether
raloxifene and
simvastatin coadministration gives added benefit beyond either monotherapy in affecting fasting lipoproteins and
apolipoproteins . -
pubmed
Each active treatment decreased non -
HDL cholesterol compared with placebo ( P & lt ; .01 ) . -
pubmed
Coadministration treatment was more effective than either monotherapy in reducing
LDL cholesterol ( P & lt ; .05 ) . -
pubmed
Coadministration treatment reduced mean
apolipoprotein B ( P & lt ; .001 ) and non -
HDL cholesterol ( P & lt ; .001 ) when compared with
raloxifene , but was not significantly different when compared with
simvastatin . -
pubmed
Coadministration therapy increased
HDL cholesterol and
apolipoprotein A1 levels compared with placebo ( P & lt ; .02 ) . -
pubmed
No significant effect on
triglycerides ,
very low density lipoprotein cholesterol , and
lipoprotein ( a ) occurred with any active treatment . -
pubmed
Combining
lapatinib (
GW572016 ) , a small molecule inhibitor of
ErbB1 and
ErbB2 tyrosine kinases , with therapeutic anti -
ErbB2 antibodies enhances apoptosis of
ErbB2 - overexpressing
breast cancer cells . -
pubmed
Treating
ErbB2 - overexpressing
breast cancer cell lines with a relatively low concentration of
lapatinib alone resulted in a minimal increase in
tumor cell apoptosis with an associated decrease in steady - state protein levels of
p - ErbB2 , p -
Akt , p -
Erk1 / 2 , and notably
survivin , compared to baseline . -
pubmed
Treatment with
trastuzumab alone had relatively little effect on
survivin and apoptosis was unaffected . -
pubmed
Combining
lapatinib with either pAb or
trastuzumab markedly downregulated
survivin protein and enhanced
tumor cell apoptosis . -
pubmed
Ifosfamide must be activated by
cytochrome P450 (
P450 ) enzymes in the liver , initially to a short lived intermediate and then to toxic metabolites that are subsequently distributed by the circulatory system . -
pubmed
In this study , we examined the feasibility of
fibrin glue , mixed with
hyaluronic acid ( HA ) as a composite scaffold . -
pubmed
These results indicate that the metabolism of
iprodione involves Phase II biotransformation in the liver ( XRE , GSTYa ) , and that this chemical has the potential to cause cell proliferation and/or inflammatory reactions ( c - fos ,
NF - kB ) , proteotoxic effects (
HSP70 ,
GRP78 ) , metabolic disruption ( CRE ) , and DNA damage (
GADD45 ,
GADD153 ) . -
pubmed
However ,
atrazine metabolites ,
deethylatrazine (
DEA ) and
deisopropylatrazine (
DIA ) , were detected in plant roots and the
AM colonization enhanced the metabolism . -
pubmed
The only drugs currently approved for the treatment of
HBV infection are
lamivudine ,
adefovir ,
entecavir , and
interferon - a. -
pubmed
After achieving steady - state exercise ,
L - NAME ( 25 mg ) was infused over 5 min to inhibit
NO synthase . -
pubmed
Dramatic clinical efficacy of
cladribine in
Rosai - Dorfman disease and evolution of the
cytokine profile : towards a new therapeutic approach . -
pubmed
Our findings show the promising efficacy of
cladribine and suggest that therapies targeting specifically
cytokines might be useful in some cases of active
RDD . -
pubmed
This was a predetermined secondary end point of a randomized clinical trial designed to assess progression of coronary artery
calcium (
CAC ) scores in the two treatment arms . -
pubmed
Mortality was borderline significantly lower in subjects randomized to
sevelamer ( 5.3/100 patient years , confidence interval ( CI ) ( 2.2 - 8.5 ) compared to those randomized to
calcium - containing binders ( 10.6/100 patient years , CI
6.3 - 14.9 ) ( P=0.05 ) . -
pubmed
The association of
systolic blood pressure (
SBP ) with the MMSE score through the follow - up period was analysed controlling for
frailty ( time to
death ) , age , gender ,
apoprotein E ,
homocysteine ,
hypertension ,
congestive heart failure , and
stroke . -
pubmed
These findings suggest the presence of the specific
vitamin C transporter
SVCT in retina and
RPE , which may be involved in the manifestation of
diabetic retinopathy . -
pubmed
Using multivariate studies , four major
CPE variables were evaluated :
Triton X-114 concentration , sample volume ,
NaCl concentration , and pH. -
pubmed
The results show that surfactant concentration and sample volume were the main variable affecting the
CPE process , with the following optimized parameters : 1 % ( w / v )
Triton X-114 concentration , 50 microL of sample volume , 6 % ( w / v )
NaCl concentration and extractions carried out at pH 7.0 . -
pubmed
Structural basis for the activity of the
RSK - specific inhibitor ,
SL0101 . -
pubmed
SL0101 is a potent and specific inhibitor of
RSK ; therefore , we performed an analysis of the structural basis for the inhibitory activity of this
lead compound . -
pubmed
In in vitro
kinase assays we found that acylation of the
rhamnose moiety and the 4 ' , 5 , and
7 - hydroxyl groups are responsible for maintaining a high affinity interaction of
RSK with
SL0101 . -
pubmed
It is likely that the
hydroxyl groups facilitate
RSK binding through their ability to form
hydrogen bonds . -
pubmed
We found that acylation of the
rhamnose moiety was essential for maintaining the selectivity for
RSK inhibition in intact cells . -
pubmed
Further , the efficacy of
SL0101 in intact cells is limited by cellular uptake as well as possible hydrolysis of the
acetyl groups on the
rhamnose moiety by ubiquitous intracellular
esterases . -
pubmed
These studies should facilitate the development of a
RSK inhibitor , based on the
SL0101 pharmacophore , as an anti -
cancer chemotherapeutic agent . -
pubmed
As rejection rates were higher in the absence of
CsA , further study of
calcineurin inhibitor - free regimens is required before general recommendations can be made . -
pubmed
In this
syndrome , we believe that high levels of circulating
epinephrine trigger a switch in intracellular signal trafficking in ventricular cardiomyocytes , from
G ( s ) protein to
G ( i ) protein signaling via the
beta ( 2 )- adrenoceptor . -
pubmed
Importantly , administration of
olomoucine , a selective
CDK inhibitor , not only suppressed astroglial proliferation and glial
scar formation , but also decreased neuronal cell
death in the ischemic boundary zone and hippocampal
CA1 region at days 1 and 30 after
MCAO . -
pubmed
Retrospective detection of
sulfur mustard exposure by mass spectrometric analysis of adducts to
albumin and
hemoglobin : an in vivo study . -
pubmed
The
albumin adduct (
S - HETE ) Cys - Pro - Tyr was detectable up to 7 days after the exposure , while the adduct to the
N - terminal valine in
hemoglobin was still detected after 28 days . -
pubmed
Remarkably , the N - terminal
valine adduct to
hemoglobin increased during the first three days , which implies that there is still free
sulfur mustard present during that time . -
pubmed
OBJECTIVE : To compare efficacy of sublingual ( S / L ) , oral and vaginal routes of
misoprostol administration for cervical priming before suction evacuation (
SE ) under local anaesthesia . -
pubmed
Autologous
PRGF activated with
calcium chloride was used to infiltrate the injured tissues . -
pubmed
Nine skilled players ( 21.0+/-2.9 yr ) underwent a graded maximal aerobic test (
GT ) where V O(2)max and
HR - V
O ( 2 ) relationship were determined . -
pubmed
V
O ( 2 ) , HR and blood
lactate ( [
La](b ) ) were recorded during a 2 x 225 s ( interspersed with 30s
rest ) 4 - a - side handball game and were compared to those measured during an 480 - s running intermittent exercise ( IE ) . -
pubmed
The HR - V
O ( 2 ) relationship during
GT was high ( r(2)=0.96 , p<0.001 ) but estimated V O(2 ) from HR was lower to that measured ( p=0.03 ) in handball , whereas there was no difference in IE . -
pubmed
The biochemical
hypercortisolemia together with the
prolactin and
androgen levels were evaluated to explore the cause of her
anovulation . -
pubmed
Adrenal
Cushing ' s syndrome was confirmed on the basis of the elevated urinary free
cortisol ( 454 microg/24h , normal range : 20 - 70 ) with a suppressed
ACTH level ( 2.0 pg / mL , normal range : 6.0 - 76.0 ) and the loss of circadian
cortisol secretion . -
pubmed
The behavior at the air / water interface and the structures of Langmuir - Blodgett monolayers at different surface pressures of rod -
coil molecules , which consist of a Y - shaped rigid aromatic segment containing peripheral
tetradecyloxy groups and a flexible
poly ( ethylene oxide ) (
PEO ) chain with 17 , 21 , 34 , or 45 repeating
ethylene oxide units ( Y17 , Y21 , Y34 , and Y45 ) , were investigated . -
pubmed
Expressed prostatic secretions ( EPS ) were obtained to determine the leukocyte count ,
PMN elastase ( ELISA ) and
citric acid concentrations ( UV method ) , and the occurrence of
C. - pubmed
The
AlkB family proteins utilize
iron ( II ) ,
alpha - ketoglutarate (
alpha - KG ) and
dioxygen to perform oxidative repair of alkylated nucleobases in DNA and RNA . -
pubmed
In addition , these two proteins can catalyze the demethylation of 3 - meU in ssRNA with a slightly higher efficiency over that of
3 - meT in ssDNA , suggesting that methylated RNAs are the preferred substrates for
FTO . -
pubmed
Nonenzymatic role of
acetylcholinesterase in neuritic sprouting : regional changes in
acetylcholinesterase and
choline acetyltransferase after neonatal
6 - hydroxydopamine lesions . -
pubmed
Parallel examinations of
choline acetyltransferase , an enzymatic marker for cholinergic nerve terminals , showed a different pattern of
6 - OHDA - induced alterations , with initial decreases in both forebrain and brainstem in males and regression toward normal by PN21 ; females were far less affected . -
pubmed
Further , the results for
choline acetyltransferase indicate that early depletion of
norepinephrine compromises development of
acetylcholine systems , consistent with a trophic role for this neurotransmitter . -
pubmed
Activated
carbon cloth (
ACC ) was used as adsorbent for the removal of cationic surfactants such as
benzyltrimethylammonium chloride (
BTMACl ) ,
benzyltriethylammonium chloride (
BTEACl ) ,
benzyltributylammonium chloride (
BTBACl ) ,
benzyldimethyldecylammonium chloride (
BDMDACl ) ,
benzyldimethyltetradecyl ammonium chloride (
BDMTDACl ) ,
benzyldimethylhexadecylammonium chloride (
BDMHDACl ) ,
N - dodecylpyridinium chloride (
N - DPCl ) and
N - cetylpyridinium chloride (
CPCl ) from aqueous solutions . -
pubmed
Ion specific effects of
sodium and
potassium on the catalytic activity of
HIV-1 protease . -
pubmed
The behavior of
HIV-1 protease in aqueous
NaCl and
KCl solutions is investigated by kinetic measurements and molecular dynamics simulations . -
pubmed
Sodium is attracted more strongly than
potassium to the
protein surface primarily due to stronger interactions with
carboxylate side chain groups of
aspartates and
glutamates . -
pubmed
These effects are of particular importance for acidic
amino acid residues at or near the active site of the
enzyme , including a pair of
aspartates at the entrance to the reaction cavity . -
pubmed
RNF122 : a novel ubiquitin ligase associated with
calcium - modulating
cyclophilin ligand . -
pubmed
From a yeast two - hybrid screen , we identified
calcium - modulating
cyclophilin ligand (
CAML ) as an
RNF122 - interacting protein . -
pubmed
The
CYP21A2 H365Y mutant exhibited minimal
21 - hydroxylase activity to convert
17 - hydroxyprogesterone to
11 - deoxycortisol or
progesterone to
11 - deoxycorticosterone . -
pubmed
Threonine ( T ) was also substituted with
alanine ( A ) at position 8 ( T1087A ) more frequently in A*03 - positive patients ( 6/12 ) compared with controls ( 2/33 , p=0.01 ) , and the double substitution of TK to
AR was also observed predominantly in
HLA - A * 03 - positive patients ( p=0.004 ) . -
pubmed
After extensive genetic and metabolic investigation , a nucleotide substitution c.2207 A G in the
POLG gene resulting in
amino acid change Asn 736Ser in exon 13 was demonstrated . -
pubmed
Antioxidant defenses consisting of contents of
reduced glutathione (
GSH ) and
glutathione disulfide (
GSSG ) and activities of
superoxide dismutase (
SOD ) ,
catalase (
CAT ) ,
glutathione reductase (
GR ) ,
selenium - dependent glutathione peroxidase (
Se - GPx ) and
glutathione S - transferase (
GST ) in liver of freshwater fish were determined and the
GSH -
GSSG ratio and content of
tGSH ( total
glutathione ) were calculated . -
pubmed
In addition , hepatic
SOD and
GSH , especially
tGSH , were sensitive to
2,4 - DCP contamination and thus , can possibly be used in early assessment of
2,4 - DCP - dominant polluted aquatic ecosystems . -
pubmed
Moreover ,
ALK5 inhibition using the
SB431542 inhibitor significantly affected
CTGF expression only at later time points whereas
ERK1 / 2 inhibition completely abrogated
CTGF expression . -
pubmed
The
Cellular Prion Protein Prevents
Copper - Induced Inhibition of
P2X ( 4 ) Receptors . -
pubmed
Although the physiological function of the cellular
prion protein (
PrP(C ) ) remains unknown , several evidences support the notion of its role in
copper homeostasis . -
pubmed
PrP ( C ) binds
Cu ( 2 + ) through a domain composed by four to five repeats of eight
amino acids . -
pubmed
Previously , we have shown that the perfusion of this domain prevents and reverses the inhibition by
Cu ( 2 + ) of the
adenosine triphosphate (
ATP)-evoked currents in the
P2X ( 4 ) receptor subtype , highlighting a modulatory role for
PrP ( C ) in synaptic transmission through regulation of
Cu ( 2 + ) levels . -
pubmed
Here , we study the effect of full - length
PrP(C ) in
Cu ( 2 + ) inhibition of
P2X ( 4 ) receptor when both are coexpressed . -
pubmed
PrP ( C ) expression does not significantly change the
ATP concentration - response curve in oocytes expressing
P2X ( 4 ) receptors . -
pubmed
However , the presence of
PrP(C ) reduces the inhibition by
Cu ( 2 + ) of the
ATP - elicited currents in these oocytes , confirming our previous observations with the
Cu ( 2 + ) binding domain . -
pubmed
Thus , our observations suggest a role for
PrP ( C ) in modulating synaptic activity through binding of extracellular
Cu ( 2 + ) . -
pubmed
We detected the activities of
GPx , GRd and
SOD in the liver , and the levels of
inflammatory mediators including
IL-1beta ,
IL-6 ,
TNF - alpha ,
COX-2 ,
MDA and
NO in the
edema paw to investigate the mechanism of action against
inflammation . -
pubmed
EO(
MeOH ) increased the activities of
SOD and
GPx in liver tissue and decreased
MDA ,
NO ,
IL-1beta ,
IL-6 ,
TNF - alpha and
COX-2 levels in paw
edema tissue at the 3rd h after lambda - carrageenan - induced inflammatory reaction . -
pubmed
It was indicated that the anti - inflammatory mechanism of EO(
MeOH ) may be due to declined levels of
NO and
MDA in the
edema paw through increasing the activities of
SOD ,
GPx and
GRd in the liver . -
pubmed
Additionally , EO(
MeOH ) decreased
IL-1beta ,
IL-6 ,
TNF - alpha and
COX-2 levels in the
edema paw . -
pubmed
EphA4 receptor (
EphA4 )
tyrosine kinase is an important regulator of central nervous system development and synaptic plasticity in the mature brain , but its relevance to the control of normal behavior remains largely unexplored . -
pubmed
In addition , immunohistological analyses revealed that some of the neurons in the ectodermal epithelium are
GLWamide- and
PRGamide - positive . -
pubmed
This inhibition is due to the binding by
Grft of high -
mannose saccharides on the surface of
gp120 . -
pubmed
AREAS COVERED :
Today an array of biologics blocking either proinflammatory
cytokines or lymphocyte activation / survival are available that enable a substantial improvement over conventional disease - modifying antirheumatic drugs ( DMARDs ) . -
pubmed
Loss of function mutations and non - conservative
amino acid replacements affected several habitat- and lifestyle - associated traits , for example , the key regulator
GacS of the switch between acute and
chronic disease phenotypes was disrupted in
strain CHA . -
pubmed
Comparison of Effectiveness of
Betamethasone gel Applied to the Tracheal Tube and
IV Dexamethasone on
Postoperative Sore Throat : A Randomized Controlled Trial . -
pubmed
This randomized controlled trial study investigated the incidence of
postoperative sore throat after general anesthesia when
betamethasone gel is applied to a tracheal tube compared with when
IV dexamethasone is prescribed . -
pubmed
RESULTS : The incidence of
sore throat was significantly lower in the
betamethasone gel group compared with the
IV dexamethasone and control groups , 1 , 6 , and 24 hours after surgery . -
pubmed
In the first day after surgery 10.7 % of the
betamethasone group had
sore throat whereas 26.7 % of the
IV dexamethasone group and 30.7 % of the control group had
sore throat . -
pubmed
CONCLUSION : We concluded that wide spread application of
betamethasone gel over tracheal tubes effectively mitigates
postoperative sore throat , compared with
IV dexamethasone application . -
pubmed
Changes in
follicle - stimulating hormone (
FSH ) ,
testosterone , sperm motility , semen volume , and sperm morphology were also examined . -
pubmed
There were no significant differences between placebo and
pregabalin groups with respect to their effects on
FSH ,
testosterone , or sperm motility . -
pubmed
However , in the presence of TALE factors ,
RNAPII remains phosphorylated on
serine 5 and
hoxb1a transcription is inefficient . -
pubmed
This triggers
P - TEFb - mediated transitioning of
RNAPII to the
serine 2 - phosphorylated form and efficient
hoxb1a transcription . -
pubmed
RESULTS : ECLS significantly improved renal (
creatinine 1.86 + - 0.91 vs 1.32 + - 0.52 mg / dl , P = 0.02 ) , hepatic (
aspartate aminotransferase 1426 + - 2176 vs 277 + - 259 U / l , P = 0.04 ;
alanine aminotransferase 982 + - 1466 vs 357 + - 447 U / l , P = 0.04 ) and
pulmonary functions ( fraction of inspired
oxygen 52 + - 18 vs 26 + - 23 % , P & lt ; 0.01 ; positive end - expiratory pressure 7 + - 3 vs 5 + - 4 mbar , P = 0.02 ) over a period of 8 + - 7 days . -
pubmed
The structure of this new
polyglucuronic acid sodium salt was analyzed by
( 13 ) C NMR spectra and HPAEC -
PAD chromatography . -
pubmed
Cyclophosphamide and
IL-12 - transduced DCs enhance the antitumor activity of
tumor antigen - stimulated DCs and reduce Tregs and MDSCs number . -
pubmed
The highest level of
tumor growth inhibition , accompanied by high cytotoxic activity of effector cells , and their increased influx into
tumor tissue , was observed after application of
cyclophosphamide in combination with BMDC / TAg and BMDC /
IL-12 . -
pubmed
AIM : To assess the effects and safety of
iron - based
phosphate binders in adult patients receiving dialysis . -
pubmed
Anthropometric parameters , serum
IL-6 ,
IL-18 ,
CRP ,
glucose ,
insulin concentrations and
insulin sensitivity / resistance indexes were evaluated . -
pubmed
Moreover , we confirmed specific electrophysiological responses from different neuronal units in the thalamus , in response to particular types of painful stimuli ( i , e. ,
formalin injection , pinch ) , which we found to be modulated by optogenetic control of the
ACC inhibitory neurons . -
pubmed
Recently , a dual - target
indol-3ylglyoxyldipeptide derivative , designed to bind to the
Translocator Protein (
TSPO ) and reactivate
p53 function via dissociation from its physiological inhibitor ,
murine double minute 2 (
MDM2 ) , has been developed as a potent GBM pro - apoptotic agent . -
pubmed
Changes in the
glucose avidity of mediastinal
nodal metastases may be a stronger predictor of survival than changes in the primary site of disease . -
pubmed
Phosphatase 2A (
PP2A ) is an important
serine and
threonine phosphatase . -
pubmed
Prospects of medium specific activity ( 177 ) Lu in targeted therapy of
prostate cancer using
( 177 ) Lu - labeled
PSMA inhibitor . -
pubmed
Targeted radionuclide therapy using
( 177 ) Lu - labeled peptidomimetic inhibitor of
prostate specific membrane antigen (
PSMA ) viz . -
pubmed
A stereoselective total synthesis of
( + ) - marrubiin has been accomplished starting from a chiral building block via the
CyNH2 - promoted Pauson - Khand reaction (
PKR ) followed by oxidative cleavage of the resultant
cyclopentenone ring . -
pubmed
OBJECTIVE : To evaluate the feasibility and image quality of low - dose
CT of the paranasal
sinuses using iterative reconstruction with adaptive - iterative dose reduction in three dimensions ( AIDR 3D ) in comparison with conventional image protocols of older scanner generations . -
pubmed
Cross - sectional
TEM investigation reveals that the utilization of the
diamond interlayer circumvented the formation of amorphous
boron nitride prior to the growth of hexagonal
boron nitride . -
pubmed
Glutamate metabolism was indicated by expression of
glutamine synthase (
GS ) ,
glutamate - aspartate transporter (
GLAST ) and
glutamate transporter-1 (
GLT-1 ) at both protein and gene levels . -
pubmed
Compared to the control group , exposure to
2 - CE could cause a dose dependent damage in astrocytes , indicated by decreased cell viability and morphological changes , and supported by decreased levels of nonprotein
sulfhydryl (
NPSH ) and inhibited activities of
Na +/ K +- ATPase and
Ca2 + - ATPase in the cells . -
pubmed
A first intravenous dose of
bisphosphonates may be associated with an acute - phase response (
APR ) . -
pubmed
Prior
bisphosphonate exposure was predictive of
APR risk and severity . -
pubmed
INTRODUCTION : Intravenous (
IV ) administration of
bisphosphonates ( BP ) , such as
zoledronate (
ZOL ) and
ibandronate (
IBN ) , may be associated with an
APR . -
pubmed
Prior oral or
IV BP use was less frequent in the
ZOL group ( 61.8 vs. 71.7 % , p = 0.005 ) . -
pubmed
Prior BP exposure was predictive of both
APR risk and severity , and lower serum
25 - hydroxy vitamin D (
25 ( OH ) D ) levels were possibly predictive of severity . -
pubmed
CONCLUSIONS : In a real - world setting ,
APR rates with
ZOL and
IBN may be higher than reported in randomised controlled trials and may differ by compound , prior BP exposure , and serum
25 ( OH ) D levels . -
pubmed
Staining techniques most commonly used included the following :
cytokeratin 7 ( 94 % ) ,
KIT ( 71 % ) ,
vimentin ( 65 % ) , colloidal
iron ( 59 % ) ,
CD10 ( 53 % ) , and
AMACR ( 41 % ) . -
pubmed
The results suggest that signaling proteins affected by
EGCG in
ovarian cancer , which include
JUN ,
FADD ,
NFKB1 ,
Bcl-2 ,
HIF1alpha , and
MMP , are involved primarily in cell cycle , cellular assembly and organization , DNA replication , etc . -
pubmed
Extended stability of the
rituximab biosimilar
CT - P10 in its opened vials and after dilution and storage in
polyolefin bags . -
pubmed
The stability of the
rituximab biosimilar
CT - P10 , in 50mL vials at a concentration of 10mg / mL , and after dilution to final concentrations of 1 and 4mg / mL and storage in
polyolefin bags at 4 C and 25 C was studied by several orthogonal and complementary methods . -
pubmed
Lung
glutathione was significantly depleted across all
CS groups , however the FD group had the most severe , ongoing oxidative stress . -
pubmed
Notably , the
HgP per unit of TSS at the burned site was an order of magnitude higher than the unburned site ( 2.66 and 0.13 ng
HgP per mg TSS , respectively ) for 8 months following the fire , resulting in elevated
HgP concentrations for the range of flow conditions , after which there was a rapid return to non - disturbed conditions . -
pubmed
We developed new photoresponsive
polydiacetylene ( PR - PDA ) molecules by incorporating a photocleavable moiety ,
6 - nitropiperonyl alcohol (
NP ) or
4,5 - dimethoxy-2 - nitrobenzyl alcohol (
DMN ) , into a self - assembling
diacetylene molecule . -
pubmed
In this paper , a
WSe2 film prepared by chemical vapor deposition ( CVD ) is transferred onto a tapered fiber , and a
WSe2 saturable absorber (
SA ) is fabricated . -
pubmed
The modulation depth of the
WSe2 SA is measured as being 21.89 % . -
pubmed
Taking advantage of the remarkable nonlinear absorption characteristic of the
WSe2 SA , a mode - locked
erbium - doped fiber laser is demonstrated at 1557.4 nm with a bandwidth of 25.8 nm and signal to noise ratio of 96 dB. -
pubmed
Interleukin-6 or
tumor necrosis factor - alpha blockade treatments were not effective , the oral administration of
colchicine was stared under the immunosuppressive treatments with
steroid and
cyclosporine A (
CyA ) . -
pubmed
The dose of
prednisolone was successfully tapered , and the elevated levels of
C - reactive protein were normalized . -
pubmed
Moreover ,
VU0155041 treatment substantially blocked
SHH - induced
cyclin D1 expression and cell proliferation , while increasing TUNEL - positive cells and the activation of apoptosis - related proteins . -
pubmed
We could estimate whether a vessel was an artery or a vein by using the S - factor obtained from the
PA images at two wavelengths , which corresponds approximately to the
haemoglobin oxygen saturation . -
pubmed
A779 attenuated
captopril - mediated improvements in
insulin sensitivity ( 23 + - 2 mg kg-1 min-1 ; P = 0.042 ) , with no effect on
body weight ( 32 + - 2 g ; P = 0.441 ) or BP ( 111 + - 7 mmHg ; P = 0.788 ) . -
pubmed
Tanshinone IIA protects lens epithelial cells from
H2 O 2 -induced
injury by upregulation of lncRNA
ANRIL . -
pubmed
In the meantime , the expression of lncRNA
ANRIL was upregulated by
Tanshinone IIA . -
pubmed
Moreover , the elevated expression of lncRNA
ANRIL induced by
Tanshinone IIA was abolished by
BAY 11 - 7082 ( an inhibitor of
NF - kappaB ) . -
pubmed
Tanshinone IIA confers its protective effects possibly via modulation of
NF - kappaB signaling and thereby elevating the expression of lncRNA
ANRIL . -
pubmed
Dihydrotestosterone inhibits
arylsulfatase B and
Dickkopf Wnt signaling pathway inhibitor ( DKK )- 3 leading to enhanced Wnt signaling in prostate epithelium in response to stromal
Wnt3A. -
pubmed
The effects of
androgen exposure on
ARSB and the impact of decline in
ARSB on Wnt signaling in prostate tissue were unknown . -
pubmed
The effects of
DHT and of
ARSB overexpression on
DKK expression were determined . -
pubmed
Exposure to
5alpha - dihydrotestosterone (
DHT ) increased
ARSB and
DKK3 promoter rmethylation , and effects of
DHT on
DKK3 expression were reversed when
ARSB was overexpressed . -
pubmed
CONCLUSIONS :
Androgen - induced declines in
ARSB and
DKK3 may contribute to prostate
carcinogenesis by sustained activation of Wnt signaling in prostate epithelium in response to stromal
Wnt3A. -
pubmed
In vitro ,
PTXL showed better ability on inhibiting cell proliferation , migration and invasion , and effectively on promoting apoptosis and arresting cell cycle in
G2 / M phase compared to
PTX . -
pubmed
Finally , we found
PTX treatment suppressed the production of
tumor necrosis factor alpah (
TNF - alpha ) ,
interleukin 6 (
IL-6 ) and
transforming growth factor beta (
TGF - beta ) and inhibited the expression of
alpha smooth muscle actin (
alpha - SMA ) and
collagen I in HKFs . -
pubmed
The activation of
protein kinase B (
AKT)/
glycogen synthase kinase 3 beta (
GSK3beta ) signaling pathway also blocked by
PTX in cultured HKFs and
keloid tissues . -
pubmed
LY294002 , a
PI3 K (
phosphatidylinositol 3 - kinase)/
AKT inhibitor , also suppressed the expression of
TNF - alpha ,
IL-6 and
TGF - beta , and simultaneously , reduced the production of
alpha - SMA and
collagen I in HKFs . -
pubmed
The inhibition of
AKT /
GSK3beta signaling pathway contribute to inhibit the generation of fibrogenic
cytokines by
PTXL on ameliorating
fibrosis progress in
keloids . -
pubmed
Mean serum
FGF23 concentration and standard deviation of participants at baseline and after
vitamin D3 supplementation were extracted to calculate standard mean difference ( SMD ) . -
pubmed
The meta - analyses found that serum intact
FGF23 increased significantly after oral
vitamin D3 supplementation in
vitamin D - deficient participants with the pooled SMD of 0.36 ( 95%CI , 0.14 , 0.57 ; p = 0.001 ; I2 of 36 % ) . -
pubmed
Serum C - terminal
FGF23 also increased after
vitamin D3 supplementation in
vitamin D - deficient participants with the pooled SMD of 0.28 although without reaching statistical significance ( 95%CI , - 0.08 , 0.65 ; p = 0.13 ; I2 of 0 % ) . -
pubmed
The present systematic review and meta - analysis revealed that serum intact
FGF23 concentration increased significantly after oral
vitamin D3 supplementation in
vitamin D - deficient participants . -
pubmed
After 1h , hepatic and splenic tissue samples were harvested for TBARS ,
CAT ,
GPx and
GSH - Rd measurement . -
pubmed
Furthermore , the hepatic and splenic activities of
CAT ,
GPx and
GSH- Rd were significantly higher in sIPC group than in IRI group . -
pubmed
BACKGROUND : A confounding issue in
[ 18F ]- NaF PET /
CT imaging of
abdominal aortic aneurysms (
AAA ) is the spill in contamination from the bone into the
aneurysm . -
pubmed
CONCLUSION : The background correction ( OSEM +
PSF + BC ) technique provided more robust AAA quantitative assessments regardless of the
AAA ROI delineation method , and thus it can be considered as an effective spill in correction method for
[ 18F ]- NaF AAA studies . -
pubmed
The extract was able to reduce total
cholesterol and non -
high - density lipoprotein cholesterol ( C - HDL ) levels and increase the C - HDL levels reduced in
diabetes , decreasing the atherogenic index and therefore the risk of suffering
CVD at the same level as
atorvastatin . -
pubmed
HRM was also used for the detection of adulteration of
olive oil with oils of different plant origin by using plastid
trnL indels and SNPs . -
pubmed
The
trnL - indels - HRM showed higher discrimination power than the trnL - SNP - HRM in determining adulteration in
olive oil . -
pubmed
Meanwhile , it remained a rejection of 95.85 %
bovine serum albumin (
BSA ) , which was also better than that of the pristine
PES membrane . -
pubmed
The
enzyme showed a great deal of stability in the presence of
ethanol ,
methanol ,
acetone ,
2 - propanol ,
dimethyl sulfoxide ,
Tween-80 and
Triton X-100 . -
pubmed
A series of
collagen fibrils with diameters of 26 - 230 nm were harvested using this process , and in situ observations under polarizing microscopy ( POM ) and using molecular dynamics simulations revealed the influence of the
NaOH /
urea system on the tendon
collagen . -
pubmed
Vesicles prepared by exposure of spheroplasts to ultrasound contained
cytochromes a , b and c , and at 50 degrees C they rapidly oxidized
NADH and
ascorbate in the presence of
tetramethyl - p - phenylenediamine . -
pubmed
Hydrolysis of
ATP by a
dicyclohexylcarbodi - imide - sensitive
adenosine triphosphatase was accompanied by a similarly directed net translocation of
H (+ ) . -
pubmed
The last result suggested an energy - conserving site in the respiratory chain between
cytochrome c and
oxygen . -
pubmed
Under anaerobic conditions the reduction of
cytochrome b by
ascorbate ( with
tetramethyl - p - phenylenediamine ) was stimulated by
ATP hydrolysis , indicating an energy - conserving site between
cytochrome b and
cytochrome c. -
pubmed
However , no reduction of
NAD (+ ) supported by oxidation of
succinate ,
malate or
ascorbate occurred , neither did it with these substrates in the presence of
ATP under anaerobic conditions , suggesting that there was no energy - conserving site between
NADH and
cytochrome b. -
pubmed
These effects were not observed when
phenazine methosulphate , which transfers electrons from
succinate dehydrogenase directly to
oxygen , was present . -
pubmed
It was concluded that in these vesicles the oxidation of
succinate was energy - dependent and that the reoxidation of reduced
succinate dehydrogenase was dependent on the outward movement of
H (+ ) by the protonmotive force . -
pubmed
This may be related to the need for an ample supply of
succinate for
haem synthesis in order to provide
cytochromes for the organism . -
pubmed
A mutant of
MP1 ,
MP5 , had increased susceptibility to
amikacin and reduced
phosphotransferase activity . -
pubmed
MP9 , a
mutant MP5 , was more resistant to
amikacin and had increased
phosphotransferase activity . -
pubmed
The mutations leading to these alterations of
amikacin susceptibility and
amikacin phosphotransferase activity were transferable with the same plasmid that was associated with
amikacin resistance and
phosphotransferase activity in
MP1 . -
pubmed
[ Scope and limitation of the analytical use of
dansyl chloride ,
II : Formation of secondary
dansyl amines by the reaction of
dansyl chloride with
N - oxides of
aliphatic tertiary amino derivatives according to the Polonovski reaction ( author 's transl ) ] . -
pubmed
In contrast ,
carbohydrate - induced preservation of labile
enzymes during freeze - drying is a fundamentally different process . -
pubmed
Using Fourier transform intra - red spectroscopy we have found that
hydrogen bonding between the
carbohydrate and the dried
protein is required for stabilization . -
pubmed
Thus , it appears that certain
carbohydrates protect dried
enzymes because these solutes serve as water substitutes for the dried
protein , by satisfying the
hydrogen bonding requirement of polar groups on the
protein 's surface . -
pubmed
From the time course of the release of
prosaposin induced by
thrombin and from the fact that weak platelet agonists ,
ADP ,
epinephrine , and
collagen , did not cause the release of
prosaposin ,
prosaposin secretion from platelets seemed to be from lysosome like granules . -
pubmed
Reduction of
Cl- in tubular fluid from 84 to 8 mM also decreased Acl from
16.1 to 9.9 mM and hyperpolarized Vbl by 12 mV. -
pubmed
Because a previous study suggested that electroneutral
NaCl reabsorption is mediated by
Na +/ H (+)- Cl -/ HCO3 - exchangers in the apical membrane , the
Cl-/
HCO3- exchange inhibitor ,
4,4 - diisothiocyanostilbene-2 - 2 - disulphonic acid (
DIDS ) was added to tubule fluid . -
pubmed
Total protein and
albumin are significantly less in neonatal serum , whereas
bilirubin ( total and direct ) is significantly higher in neonatal serum than in adult serum ( P less than 0.01 ) . -
pubmed
The parameters studied included blood
serotonin levels ,
monoamine oxidase (
MAO ) activity in the blood serum , as well as urinary excretion of
adrenaline ,
noradrenaline ,
dopamine ,
DOPA and
5 - HOIAA . -
pubmed
Following a course of AP they presented a compensatory increase in
MAO activity , normalization of blood
serotonin levels and of the urinary excretion of
noradrenaline ,
dopamine and
DOPA . -
pubmed
Before treatment , serum free T4 correlated significantly , either positively or negatively , with the basal metabolic rate ( BMR ) , duration of the achilles tendon reflex (
ATR ) , resting pulse rate ( RPR ) , serum total
cholesterol ( T -
cholesterol ) , ratio of the pre - ejection period to the left ventricular ejection time (
PEP / LVET ) , serum
high density lipoprotein (
HDL ) and
creatine phosphokinase (
CPK ) in the descending order . -
pubmed
A series of 64 punch biopsies collected from women prospectively followed - up for cervical
Human papillomavirus ( HPV ) infections ( with and without
CIN ) , and 38 control biopsies (
normal epithelia , and classical
CIN ) were analysed for expression of
filaggrin ( a
histidine - rich protein constituent of keratohyalin granules ) using the ABC technique and polyclonal antibody . -
pubmed
Reversal of
scopolamine - induced
amnesia of passive avoidance by pre- and
post - training
naloxone . -
pubmed
Post - training administration of
naloxone ( 3 mg / kg ) as a single or as a split dose also attenuated the
scopolamine - induced
amnesia . -
pubmed
Control experiments indicated that neither an increase in
pain sensitivity ( pre - training
naloxone ) nor an induced aversive state (
post - training
naloxone ) appear to be responsible for the influence of
naloxone on the
scopolamine - induced
retention deficit . -
pubmed
Although the peroxidative metabolism of
benzidine and other carcinogenic
arylamines has been regarded as a significant pathway in extrahepatic tissues , the mechanism of
peroxidase - mediated covalent binding to DNA has remained unclear . -
pubmed
In this study , we have compared the metabolic activation of
benzidine by
prostaglandin H synthase ,
horseradish peroxidase ,
chloroperoxidase , and
lactoperoxidase . -
pubmed
All four
peroxidases mediated the binding of
benzidine to DNA and equimolar amounts of
hydrogen peroxide ( or
arachidonic acid ) and
benzidine were required for the maximal binding in the system with either
horseradish peroxidase or
prostaglandin H synthase . -
pubmed
Two major and one minor
deoxyguanosine adducts ( P - I ,
P - III and P - IV , respectively ) were isolated after the enzymatic hydrolysis of the benzidine - modified DNA . -
pubmed
The interrelationships of
alcohol dehydrogenase and the
aldehyde dehydrogenases in the metabolism of
ethanol in liver . -
pubmed
In 10 hemodialyses (
HD ) with
cuprophan ( CU ) and 10 with
polyacrylonitrile (
PAN ) , signs of complement activation were investigated by following arterial and venous levels of
C3a ,
C3d and
C5a , in order to propose a marker of bioincompatibility . -
pubmed
HD using
PAN membranes were associated with a lower
C3a ,
C3d and
C5a generation and fewer dialyses generating these products . -
pubmed
The concentrations of
glycerol and
phosphatidylglycerol in amniotic fluid were measured by using a coupled enzymatic assay with and without
phospholipase D (
EC 3.1.4.4 ) . -
pubmed
( 3 ) Exercise - induced increases in
O2 uptake and blood
lactate concentration were both attenuated by LBNP and exaggerated by
LPP . -
pubmed
The present study was designed to determine whether the central sympatholytic effects of
ketanserin result from the ability of the drug to block
serotonin2 or alpha-1 adrenergic receptors . -
pubmed
These data , along with earlier results from our laboratory , provide strong evidence to indicate that
ketanserin produces a centrally mediated inhibition of sympathetic nerve discharge as a result of the ability of the drug to block
alpha-1 adrenergic receptors . -
pubmed
Actual and absolute deviations from the reference standard demonstrate that the Accuchek bG and Glucoscan 2000 monitors provide relatively unbiased estimates of blood
glucose , whereas the Glucokey , Glucochek
II , Glucometer
II , and Trendsmeter generally underestimate the true values . -
pubmed
Synthesis of
cytochromes and
heme by mutant
H-4 was dependent upon
delta - aminolevulinate ; this
strain did not form
bacteriochlorophyll either with or without
delta - aminolevulinate and , consequently , grew only under aerobic conditions . -
pubmed
The mutants differed in their ability to take up radioactive
delta - aminolevulinate from the external environment ; mutant
H-5 was less active than mutant
H-4 or the wild type . -
pubmed
spheroides made only one form of
delta - aminolevulinate synthase , which provided
delta - aminolevulinate for
bacteriochlorophyll and
heme synthesis . -
pubmed
A rapid microassay of
lysozyme activity with reduced chitin
oligosaccharides as substrate . -
pubmed
As the serum
digoxin level reached 21.7 ng / ml
digoxin - specific
Fab - antibody - fragments were used to bind free serum
digoxin . -
pubmed
pneumophila phagosome and monocyte lysosomes were investigated by prelabeling the lysosomes with
thorium dioxide , an electron - opaque colloidal marker , and by
acid phosphatase cytochemistry . -
pubmed
USA 78 , 2717 - 2721 , 1981 ) , we conclude that the
tryptophan codon UGG in position 234 has been changed into a UGA nonsense codon in the
CyOnB mutant . -
pubmed
The analyses also demonstrate that the importance of
Na + entry across the basolateral membrane is strongly dependent upon transepithelial potential , cmNa and
csK ; under certain conditions , net
Na + entry could be appreciably greater across the basolateral than across the apical membrane . -
pubmed
Lamellar body
phospholipases hydrolyse
dipalmitoyl phosphatidylcholine ( containing 10 %
phosphatidylglycerol and thus approximating the
phospholipid composition of mature lamellar bodies ) at only 1 % of their rate of hydrolysis of
unsaturated molecules ( Tables 1 and 2 ) . -
pubmed
The synthesis of
peroxides was estimated by the measurement of
leukotriene B4 and
C4 (
LTB4 ,
LTC4 ) . -
pubmed
Axial and sagittal
T1 - weighted images of the lumbar spine were obtained , pre- and
post -
Gd - DTPA ( 0.1 mmol / kg ) administration . -
pubmed
Omeprazole inhibits
ATPase activity also in isolated plasma - membrane vesicles . -
pubmed
Progestins were dissolved in
propylene glycol and
IV injected through an indwelling jugular catheter . -
pubmed
alpha - Naphthoflavone , a known inhibitor of
P4501A - related activities , did not affect these procarcinogen activation in fetal liver microsomes . -
pubmed
Furthermore , we analysed the effects of the non - selective ( A / B )
endothelin receptor antagonist ,
bosentan ( 6 and 100 mg kg-1 day-1 ) on mean arterial blood pressure , water and
sodium excretion and
glomerular filtration rate . -
pubmed
Moreover DNA cleavage can be identified by electrophoresis and by specific labeling using in situ nucleotidyltransferase assay ( ISNT ) ,
terminal deoxynucleotidyltransferase - mediated
dUTP nick - end labeling technique ( Tunel ) , or by Elisa . -
pubmed
[ Percutaneous
alcohol instillation therapy (
PAI ) and chemoembolization in extensive
hepatocellular carcinoma ] . -
pubmed
Following measurements were done : quantitative computed tomography , radiological estimation of thoracic and lumbal spinal column and hands , in serum total
alkaline phosphatase ,
calcium ,
phosphate , in urine excretion of
calcium ,
phosphate and
creatinine . -
pubmed
Nitric oxide (
NO ) , a gaseous molecule synthesized in the arteriolar endothelium from the
amino acid L - arginine (
L - arg ) , has been identified as the previously described
Endothelium - Derived Relaxing Factor ( EDRF ):
nitroderivatives such as
nitroglycerin are known to induce vasodilation via
NO release . -
pubmed
The first step of
glycosylphosphatidylinositol biosynthesis is mediated by a complex of
PIG - A ,
PIG - H ,
PIG - C and
GPI1 . -
pubmed
Biosynthesis of
glycosylphosphatidylinositol (
GPI ) is initiated by transfer of
N - acetylglucosamine (
GlcNAc ) from
UDP -
GlcNAc to
phosphatidylinositol (
PI ) . -
pubmed
PIG - L , which is involved in the second step in
GPI synthesis ,
GlcNAc -
PI de - N - acetylation , did not associate with the isolated complex . -
pubmed
The protein complex had
GPI -
GlcNAc transferase (
GPI - GnT ) activity in vitro , but did not mediate the second reaction . -
pubmed
These results suggest that
GPI - GnT recognizes the
fatty acyl chains of
PI . -
pubmed
Apoptosis in 30 autologous PBSC harvests mobilised by
cyclophosphamide +
G - CSF or standard chemotherapy +
G - CSF was analysed immediately after collection by dual - colour flow cytometry with
CD34 PE and
annexin V FITC . -
pubmed
Cyclophosphamide +
G - CSF significantly increased the yield of
CD34 + cells but did not increase apoptosis . -
pubmed
The heterologously expressed
synthases produce
( + ) - bornyl diphosphate ,
1 , 8 - cineole , and
( + ) - sabinene , respectively , as their major products from
geranyl diphosphate . -
pubmed
The
bornyl diphosphate synthase also produces significant amounts of
( + ) - alpha - pinene ,
( + ) - camphene , and
( + /-)- limonene . -
pubmed
The
1,8 - cineole synthase produces significant amounts of
( + ) - and
( -)- alpha - pinene ,
( + ) - and
( -)- beta - pinene ,
myrcene and
( + ) - sabinene , and the
( + ) - sabinene synthase produces significant quantities of
gamma - terpinene and
terpinolene . -
pubmed
Plasmodial
hydroperoxide metabolism displays similarities to the mammalian system in comprising
glutathione biosynthesis ,
glutathione reductase , and at least one
glutathione peroxidase homolog having the active site
selenocysteine replaced by
cysteine . -
pubmed
This observation indicates that
PrRP may regulate
oxytocin secretion . -
pubmed
These results suggest that the
PrRP acts as a neuro - modulator of the function of magnocellular neurons , especially
oxytocin neurons , in the brain . -
pubmed
In view of the conflicting data in the literature concerning the effect of
calcium channel antagonists on autonomic balance , we evaluated the effect of
verapamil on heart rate and blood pressure variability , and on baroreflex sensitivity . -
pubmed
Hyperbaric
oxygen treatment attenuates
cytokine induction after massive
hemorrhage . -
pubmed
We investigated the effect of hyperbaric
oxygen treatment ( HBO ) on
cytokine induction after
hemorrhage , because
hypoxia induces
cytokines in vitro . -
pubmed
Time course changes in plasma endotoxin level , arterial
ketone body ratio ( AKBR ) , serum
tumor necrosis factor (
TNF ) ,
interleukin-6 (
IL-6 ) , and their hepatic mRNA were detected in the three groups . -
pubmed
HBO significantly attenuated decreases in AKBR after
hemorrhage with a significant reduction of mortality and
cytokine induction . -
pubmed
These results indicate that
HBO attenuated the
cytokine induction after
hemorrhage by improving
liver ischemia , and they suggest that tissue
hypoxia may be responsible , at least in part , for
cytokine induction after massive
hemorrhage . -
pubmed
Reaction with , and fine structural recognition of
polyamines by
human IgE antibodies . -
pubmed
Human IgE antibodies from nine
allergic subjects were found to react with
poly - L - lysine (
PLL ) and other
polyamines . -
pubmed
Radioimmunoassay inhibition studies indicated that the two
amino groups , but not the
carboxyl , in
lysine contributed to the
antibody binding and
4 - aminomethyl-1,8 - octanediamine , a compound containing three
primary amino groups , was a better inhibitor than compounds containing only two
primary amino groups . -
pubmed
Ethylamine showed weak but clear inhibition indicating that even a single
amino group could bind to the
antibody combining site . -
pubmed
Inhibition studies with compounds containing an
amino and a
carboxyl group at different distances revealed that an adjacent
carboxyl group interfered with recognition of the
amino group by some
IgE antibodies . -
pubmed
IgE binding to
PLL was examined at different pHs and ionic strengths . -
pubmed
These results indicated that binding of specific
antibody to
polyamine was due to electrostatic interactions of positively charged
amino groups in the
polyamine with the
antibody combining site . -
pubmed
This non - processed
lipase had 50 % residual activity after 2 h at 50 degrees C , while the residual activity of the processed
lipases was only 10 % after 30 - 45 min of incubation at 50 degrees C. The
CD spectrum of the non - processed type of
lipase at 222 nm was almost unchanged by heating , suggesting that this group of
lipases had a very rigid structure and that the
peptide bond between the A- and B - chain contributed to maintain this rigid structure . -
pubmed
On the other hand , the length of the
sugar chain bound to the
lipase had no effect on the thermal stability . -
pubmed
Cyclosporine impairs the
guanylyl cyclase activity of the
natriuretic peptide receptor in the glomerulus . -
pubmed
The
guanylyl cyclase activity in the glomerulus from the
CsA group was attenuated compared with that from the control . -
pubmed
Direct application of
CsA to normal glomerular membrane completely abolished the
ANP - induced
guanylyl cyclase activation . -
pubmed
Binding studies , using
( 125 ) I -
ANP , revealed that B(max)was decreased in the
CsA group , while K(d)was not affected in the glomerulus . -
pubmed
These results indicate that
CsA impairs the
guanylyl cyclase activity mainly in the glomerulus by the decrease in
NPR population and/or by direct inhibition , suggesting that the
ANP /
NPR system might be involved in
CsA - induced
nephrotoxicity . -
pubmed
BACKGROUND :
Na + : HCO3 - cotransporters mediate the transport of
HCO3 - into or out of the cell . -
pubmed
Expression studies in oocytes indicated that
NBC-3 mediates
Na - dependent
HCO3 - cotransport . -
pubmed
CONCLUSIONS : Based on its distribution and electroneutrality , we propose that
kNBC-3 mediates the transport of
HCO3 - into the cells . -
pubmed
NO synthase (
NOS ) catalyses the conversion of
L - arginine to
L - citrulline in the presence of
oxygen and
NADPH - diaphorase (
NADPH - d ) . -
pubmed
After the hearts were excised , the
NADPH - d co - enzyme was visualized by a histochemical method and the endothelial isoform of
NOS was localized by immunohistochemistry . -
pubmed
The
eNOS visualization , which involved immunoprecipitation , and the
NADPH - d visualization , which involved histochemical staining , were both diminished in endothelial cells of the vascular wall of diabetic hearts , compared to non - diabetic hearts . -
pubmed
The residues of additives and other chemicals in 23 kinds of
silicone rubber products for food contact use , including nipples , packing and spatulas , were investigated by
GC /
MS . -
pubmed
Proteoglycan ( PG ) content of cartilage was measured by a
dimethylmethylene blue assay of
papain digests . -
pubmed
Ribonucleotide reductase (
RNR ) is the enzyme performing de novo production of the four
deoxyribonucleotides needed for DNA synthesis . -
pubmed
The smaller of the
homodimers , denoted
R2 , contains a
di - iron carboxylate site which , upon reaction with molecular
oxygen , generates a stable
tyrosyl radical needed for catalysis . -
pubmed
The present structure provides evidence that
manganese is not oxidized by the
protein , in agreement with recent biochemical data , and that no obvious structural abnormalities are seen in the oxidized and reduced
iron - containing forms , giving further support that the protein is indeed an
iron - dependent
RNR R2 . -
pubmed
ammoniagenes R2 also reveals an additional water molecule bridging the radical and the
iron site , which has not previously been seen in any other
R2 structure and which might have important mechanistic implications . -
pubmed
Appropriate recommendations and correct interpretations of the results of routine thyroid scans and the usefulness of other scintigraphic studies with 111In -
octreoscan ,
99mTc - MIBI ,
99mTc - DMSA ,
18FDG and radiolabeled monoclonal anti -
carcinoembryonic antigen antibodies were discussed in the light of the present thyroid diagnostic studies . -
pubmed
aegypti
KAT (
AeKAT ) has four potential N - glycosylation sites (
Asn - Xxx - Trp /
Ser ) and a typical mitochondrial leader sequence consisting of 49
amino acids at its NH(2)-terminus with a putative cleavage site between
Met-49 and
Ser-50 . -
pubmed
Using
PEI vector , both pancreatic primary
tumor growth and metastatic
tumor growth were also significantly slackened as compared with both
LacZ - treated and untreated control groups ( P & lt ; 0.02 ) . -
pubmed
Three points are discussed : ( 1 ) the different conformations of
CsA and the question of its biologically active form ; ( 2 ) the
Fab -
CsA recognition mechanism ; and ( 3 ) the relationship between structure and binding properties of
CsA analogs . -
pubmed
By use of basic coordination chemistry ,
4 -( 3 - quinolinoazo ) hydroxybenzoic acid (
QABA ) and derivatives were successfully conjugated to
ovalbumin ,
bovine serum albumin , and
alkaline phosphatase at a site that was distinct from the metal binding site . -
pubmed
A single
amino acid alteration in
cytoplasmic domain determines IL-2 promoter activation by ligation of
CD28 but not
inducible costimulator (
ICOS ) . -
pubmed
Together , these observations lead us to believe that the difference of a single
amino acid , which affects
Grb2 binding ability , may define a functional difference between the
CD28- and
ICOS - mediated costimulatory signals . -
pubmed
Proliferation , cytotoxicity , and
cytokine secretion were measured by
[ ( 3 ) H ] thymidine incorporation ,
( 51 ) Cr release , and enzyme - linked immunosorbent assay , respectively . -
pubmed
The specificity of the
drug T cell receptor interaction was studied using
CBZ metabolites and other structurally related compounds . -
pubmed
The
T cell receptor of certain clones could accommodate some
CBZ metabolites , but no cross - reactivity was seen with other anticonvulsants or structural analogs . -
pubmed
C - reactive protein (
CRP ) , a marker of
systemic inflammation and an independent predictor of cardiovascular mortality in the general population , and
asymmetric dimethylarginine (
ADMA ) , an endogenous inhibitor of
NO synthase , are important risk factors for
cardiovascular disease and mortality in the
ESRD population . -
pubmed
Both
CRP and
ADMA have been shown to be associated with increases in the incidence and progression of
atherosclerotic lesions in carotid arteries , as evaluated by high - resolution Doppler ultrasonography . -
pubmed
At E18 and
P0 -
P2 , the in vitro frequency decreased by around 50 % under
hypoxia , increased by 40 - 50 % under
noradrenaline or
substance P and was permanently depressed by the pontine A5 areas . -
pubmed
At
E16 however ,
hypoxia had no effects , both
noradrenaline and
substance P drastically increased the frequency and area A5 inhibition was not expressed at this time . -
pubmed
The average drop in total
cholesterol was 24.8 mg / dL ( 9.9 % ) ,
LDL 15.3 mg / dL ( 11.4 % ) , and
triglycerides 38 mg / dL ( 9.9 % ) . -
pubmed
The effects of
ribonuclease and other basic proteins on the incorporation of
C14 - L - leucine into liver proteins in vitro . -
pubmed
The
NADPH - oxidase is a plasma membrane enzyme complex that enables phagocytes to generate
superoxide in order to kill invading pathogens , a critical step in the host defense against
infections . -
pubmed
The
oxidase transfers electrons from cytosolic
NADPH to extracellular
oxygen , a process that requires concomitant H+ extrusion through depolarization - activated H+ channels . -
pubmed
Whether
H + fluxes are mediated by the
oxidase itself is controversial , but there is a general agreement that the
oxidase and
H + channel are intimately connected . -
pubmed
Oxidase activation evokes profound changes in whole - cell
H + current (
IH ) , causing an approximately -40 - mV shift in the activation threshold that leads to the appearance of inward
IH . -
pubmed
Run - down of both
IH and Ie was delayed , and sometimes prevented , by cytosolic
ATP and
GTP - gamma - S. A good correlation was observed between the amplitude of Ie and both inward and outward
IH when a stable driving force for e- was imposed . -
pubmed
Combined application of
NADPH and
DPI reduced the inward IH amplitude , even in the absence of concomitant
oxidase activity . -
pubmed
Etoposide , a substrate of both
P - glycoprotein and
CYP3A , was used as a model drug . -
pubmed
Of special significance from a clinical perspective is also
CD26 effect on
glucose metabolism through its
DPP IV activity and its potential role as a therapeutic target in
diabetes . -
pubmed
A number of experimental and clinical studies have demonstrated the beneficial effects of
trimetazidine , a drug that inhibits the long - chain mitochondrial
3 - ketoacyl coenzyme A thiolase enzyme in the myocyte , resulting in a shift from
fatty acid oxidation to
glucose oxidation . -
pubmed
The products of
heme catalysis ,
biliverdin /
bilirubin ,
carbon monoxide (
CO ) , and
iron ( that induces
apoferritin ) mediate the beneficial effects of
HO-1 . -
pubmed
HO-1 was up - regulated by
cobalt - protoporphyrin ( 5 mg / kg , intraperitoneally ) . -
pubmed
RESULTS :
Cobalt - protoporphyrin treatment resulted in significant up - regulation of
HO-1 protein in mucosal and submucosal cells . -
pubmed
CONCLUSIONS : We conclude that heightened
HO-1 expression or administration of
biliverdin ameliorates dextran
sodium sulfate - induced experimental
colitis . -
pubmed
Novel therapeutic strategies based on
HO-1 and/or
biliverdin administration may have use in
inflammatory bowel disease . -
pubmed
Bioactivity in vitro tests were excellent : ( a ) rapid and pronounced formation of
hydroxyapatite occurred when the scaffolds were immersed in simulated body fluid (
SBF ) , and ( b ) outstanding proliferation of osteoblasts was registered . -
pubmed
Amyloid deposition was histologically confirmed , and severe inflammatory activity was found in and at the margins of the
amyloidomas , the latter correlating in
CT with the halo sign . -
pubmed
The increase that occurred during the administration partially remained in the
Ca ( 2 +)-free medium and was blocked by
2 - aminoethoxydiphenyl borate (
2 - APB ) , an
IP ( 3 )- receptor blocker , indicating that the source of
Ca ( 2 + ) for the increase could be ascribed to the intracellular store . -
pubmed
The effects on the [
Ca ( 2 +)]( i ) were blocked by pretreatment with
pyridoxal phosphate-6 - azophenyl-2 ' , 4 ' disulfonic acid , a
P2 - receptor antagonist . -
pubmed
About 65 % of thymus
BuChE activity was extracted with a saline buffer and 30 % with 1 %
Triton X-100 . -
pubmed
Sedimentation analyses and
phenyl -
agarose chromatography showed that thymus contained amphiphilic
BuChE monomers ( G(1)(A),44 % ) and dimers ( G(2)(A),33 % ) , and hydrophilic tetramers ( G(4)(H),23 % ) . -
pubmed
To identify specific substrates , a mutant of
PKCalpha ( M417A ) was constructed at the
ATP binding site such that it could bind a sterically large
ATP analogue derivatized through the N6
amino group of
adenosine (
[ gamma-32P ]- N6 - phenyl - ATP ) . -
pubmed
Because this analogue could be utilized by the mutant
kinase but not by wild - type
PKCalpha ( or presumably other
protein kinase ) to phosphorylate peptide or protein substrates ,
32P - labeled products were the direct result of the mutant
PKCalpha . -
pubmed
We investigated five functional polymorphisms in the
transforming growth factor beta1 gene ( -800 G / A , -509 C / T , codon 10
Leu /
Pro , codon 25
Arg /
Pro and codon 263
Thr /
Ile ) in relation to arterial stiffness in a population - based study . -
pubmed
The results of this study show that the
TGF - beta1 -800 G / A , -509 C / T , codon 10
Leu /
Pro and codon 25
Arg /
Pro polymorphisms are not associated with arterial stiffness . -
pubmed
Specifically ,
hypoxia markedly increased
LOX protein expression ; however , catalytic activity (
beta - aminopropionitrile inhibitable
hydrogen peroxide production ) was significantly reduced under hypoxic conditions . -
pubmed
This work demonstrates that both
hypoxia and reoxygenation are necessary for
LOX catalytic activity which facilitates
breast cancer cell migration through a
hydrogen peroxide - mediated mechanism ; thereby illuminating a potentially novel mechanism by which poorly invasive
cancer cells can obtain metastatic competency . -
pubmed
Immobilization of the
pronase enzymes facilitated their removal from the
glycopeptide preparations , and was found to prevent enzyme
autolysis while leaving the proteolytic activities of
pronase intact . -
pubmed
Monitoring the progress of
glycoprotein digestion over time allowed detailed tracking of successive
amino acid cleavages about the sites of glycan attachment , and provided a more complete protein glycosylation profile than any single representative time point . -
pubmed
Separation of
beta - receptor blockers and analogs by capillary liquid chromatography (
CLC ) and pressurized capillary electrochromatography ( pCEC ) using a
vancomycin chiral stationary phase column . -
pubmed
Enantiomeric separation of chiral pharmaceuticals was carried out by means of in capillary liquid chromatography (
CLC ) and pressurized capillary electrochromatography ( pCEC ) using a
vancomycin chiral stationary phase ( CSP ) . -
pubmed
Enantiomeric resolution of fifteen
beta3 - receptor blockers and analogs was studied by polar organic
CLC mode and reversed - phase pCEC mode using mobile phases containing
methanol -
isopropanol -
acetic acid -
triethylamine and
TEAA buffer -
methanol , respectively . -
pubmed
Good enantiomeric resolution was achieved by
CLC mode for
propranolol ,
celiprolol ,
esmolol ,
bisoprolol ,
atenolol ,
metoprolol and
carteolol using
methanol -
isopropanol -
acetic acid -
triethylamine ( 70:30:0.05:0.05 , v / v / v / v ) as mobile phase and for
clenbuterol ,
bambuterol ,
terbutaline , and
salbutamol using
methanol -
isopropanol -
acetic acid -
triethylamine ( 50:50:0.05:005 or 50:50 : 0.025:0.05 , v / v / v / v ) as mobile phase . -
pubmed
Acetone extract ( 92microg GAE / mL ) increased
QR activity 2.8 - fold , while
ethanol ( 120microg GAE / mL ) and water ( 1000microg GAE / mL ) extracts increased
QR activity by 1.35- and 1.56 - fold , respectively . -
pubmed
Peritoneal fluid samples were centrifuged at 600 x g for 10 min and 4 degrees C , and the supernatant was incubated at 56 degrees C for 30 min in a
water bath to inactivate the
complement protein . -
pubmed
After a simple step of
protein precipitation using
methanol , plasma samples were analyzed by reverse phase high - performance liquid chromatography - electrospray ionization - tandem mass spectrometry ( HPLC - ESI -
MS /
MS ) with
valsartan as the internal standard ( I.S. ) -
pubmed
valsartan were separated on a Venusil MP
C18 analytical column using methanol-10mM
ammonium acetate ( 75:25 , v / v , pH 3.0 ) as mobile phase at a flow rate of 1.0 mL / min . -
pubmed
Interaction of
[ Rh ( 2 )( O ( 2 ) CCH ( 3 ))( 4 )( H ( 2 ) O )( 2 ) ] and
[ Rh ( 2 )( O ( 2 ) CCH ( OH ) Ph )( 2 )( phen )( 2 )( H ( 2 ) O )( 2 )]( O ( 2 ) C - CH ( OH ) Ph )( 2 ) With
Sulfhydryl Compounds and
Ceruloplasmin . -
pubmed
The interaction of binuclear
rhodium ( II ) complexes
[ Rh ( 2 )( OOCCH ( 3 ))( 4 )( H ( 2 ) O )( 2 ) ] ,
[ Rh ( 2 ){ OOCCH ( OH ) Ph }( 2 )( phen )( 2 )( H ( 2 ) O )( 2 ) ] { OOCCH ( OH ) Ph }( 2 ) ,
[ Rh ( 2 )( OOCCH ( 3 ))( 2 )( bpy )( 2 )( H ( 2 ) O )( 2 )]( OOCCH ( 3 ))( 2 ) and
[ Rh ( 2 ) Cl ( 2 )( OOCMe )( 2 )( bpy )( 2 )]( 3H ( 2 ) O ) with
ceruloplasmin ,
cysteine ,
glutathione and coenzyme A have been investigated using . -
pubmed
Complex
[ Rh ( 2 ){ OOCCH ( OH ) Ph }( 2 )( phen )( 2 )( H ( 2 ) O )( 2 ) ] strongly changes structure of
ceruloplasmin leading to the decrease of of alpha - helix content and loss of
oxidase activity . -
pubmed
Directly relating reduction energies of gaseous
Eu ( H2O ) n ( 3 + ) , n = 55 - 140 , to aqueous solution : the absolute
SHE potential and real proton solvation energy . -
pubmed
In solution , half - cell potentials are measured relative to other half - cells resulting in a ladder of thermodynamic values that is anchored to the standard
hydrogen electrode (
SHE ) , which is assigned an arbitrary value of exactly 0 V. A new method for measuring the absolute
SHE potential is introduced in which reduction energies of
Eu ( H ( 2 ) O )( n )( 3 + ) , from n = 55 to 140 , are extrapolated as a function of the geometric dependence of the cluster reduction energy to infinite size . -
pubmed
Losses of antioxidant activity ,
TP ,
flavanol monomers , and
procyanidins were associated with an increased extractable pH of the baked cakes . -
pubmed
The adsorbents from
pyrolusite - supplemented sludges performed better in dye removal than those without mineral addition , with the
carbon from
pyrolusite - augmented sludge
T2 presenting a significant increase in maximum adsorption capacity of 50mg / g. The properties of the adsorbents were improved during
pyrolusite - catalyzed pyrolysis via enhancement of mesopore production , thus the mesopore channels may provide fast mass transfer for large molecules like dyes . -
pubmed
Estimates of landfill gas (
LFG ) collection efficiency are required to estimate
methane emissions and the environmental performance of a solid waste landfill . -
pubmed
Apolipoprotein CIII bound to
apoB - containing lipoproteins is associated with small , dense
LDL independent of plasma
triglyceride levels in healthy men . -
pubmed
OBJECTIVE : Plasma levels of both
apolipoprotein CIII (
apoCIII ) and small , dense
LDL particles are associated with plasma
triglyceride (
TG ) concentrations , and are markers for
cardiovascular disease (
CVD ) risk . -
pubmed
Stepwise multiple regression analysis showed that
LpB : CIII was significantly associated with small , dense
LDL (
LDL3+
LDL4 ) levels after adjusting for age ,
BMI , plasma levels of
TG ,
apoB , and
HDL -
cholesterol . -
pubmed
The objective of this study was to determine the genotoxic activity of water after UV /
H ( 2 ) O ( 2 ) oxidation and
GAC filtration . -
pubmed
Pre - treated surface water from three locations was treated with UV /
H ( 2 ) O ( 2 ) with medium pressure ( MP ) lamps and passed through granulated activated
carbon (
GAC ) . -
pubmed
The results indicate that UV /
H ( 2 ) O ( 2 ) treatment may lead to the formation of genotoxic by - products , which can be removed by subsequent
GAC filtration . -
pubmed
One of the more prevalent views is that immunodominance is driven by passive T cell competition for space on
antigen presenting cells ( APCs ) or for access to specific
MHC : epitope complexes on the surface of APCs . -
pubmed
On the other hand , the non - natural
amino acids are not metabolized but are taken up through both the
L - type transporter and the energy - dependent A - type transporter ( 7 ) . -
pubmed
Sequencing of the
mevalonate kinase gene revealed 2 mutations , leading to
amino acid substitutions : one already described ( V3771 ) and R40W : never reported before . -
pubmed
Four biopsies were taken during
bisphosphonate therapy and one biopsy was taken after one year of
strontium ranelate (
SR ) treatment . -
pubmed
CONCLUSIONS / SIGNIFICANCE : Microstructural data revealed a notable increase in bone volume fraction after one year of
SR treatment compared to the
bisphosphonate treatment period . -
pubmed
Administration of
SR following
bisphosphonates led to a maintained mineralization profile with an uptake of
strontium on the bone surface level . -
pubmed
This was achieved by dispersion polymerization of
methacrylic acid (
MAA ) ,
polyethylene glycol (
PEG ) and different chitosan (
CS ) grades in the presence of cross linking agent
ethylene dimethacrylate (
EDMA ) and polymer initiator
potassium persulphate . -
pubmed
Method development was carried out by varying formulation parameters such as type of
CS , ratio of
PEG to
CS , quantity of solvent and polymer initiator . -
pubmed
These findings closely resembled the distribution of signal loss on
T2 - weighted MRI seen after SPIO injection reflecting intravascular accumulation of
iron particles trapped in vessel
thrombi as confirmed histologically . -
pubmed
Proapoptotic protein
Bim is differentially required during thymic clonal deletion to ubiquitous versus tissue - restricted
antigens . -
pubmed
The proapoptotic
Bcl-2 family member
Bim is important for negative selection by inducing apoptosis in thymocytes receiving a strong signal through their
antigen receptor . -
pubmed
Unlike
UbA , clonal deletion to tissue - restricted
antigens (
TRAs ) requires positive selection and
CCR7 - mediated migration to the medulla . -
pubmed
Serum
NGAL correlated with age , haematocrit , leukocyte , platelet and erythrocyte count , eGFR ,
creatinine , fasting
glucose ,
HbA ( 1c ) ,
fibrinogen ,
SBP , and
diabetes duration . -
pubmed
In multiple regression analysis kidney function (
eGFR ,
creatinine clearance or
creatinine ) ,
cystatin C and
SBP were predictors of serum
NGAL . -
pubmed
In the present paper ,
xanthohumol exhibited more - potent activity in inhibiting
platelet aggregation stimulated by
collagen . -
pubmed
Xanthohumol inhibited platelet activation accompanied by relative [
Ca ( 2 +)]( i ) mobilization ,
thromboxane A ( 2 ) formation ,
hydroxyl radical (
OH ( ) ) formation , and
phospholipase C ( PLC ) gamma2 ,
protein kinase C (
PKC ) ,
mitogen - activated protein kinase (
MAPK ) , and
Akt phosphorylation . -
pubmed
Neither
SQ22536 , an inhibitor of
adenylate cyclase , nor
ODQ , an inhibitor of
guanylate cyclase , reversed the
xanthohumol - mediated inhibitory effect on
platelet aggregation . -
pubmed
This study demonstrates for the first time that
xanthohumol possesses potent antiplatelet activity which may initially inhibit the
PI3 - kinase /
Akt ,
p38 MAPK , and
PLCgamma2 -
PKC cascades , followed by inhibition of the
thromboxane A ( 2 ) formation , thereby leading to inhibition of [
Ca ( 2 +)]( i ) and finally inhibition of
platelet aggregation . -
pubmed
The optical response of LCs changed from a
bright to dark appearance when an aqueous solution of
lipase was in contact with a nematic LC ,
4 - cyano-4 '- pentylbiphenyl (
5CB ) , that was doped with
glyceryl trioleate , which is a
glyceride that can be enzymatically hydrolyzed by
lipase . -
pubmed
We did not observe a
bright - to - dark shift in the optical appearance of LCs when pure
5CB was immersed into the
lipase solution . -
pubmed
Moreover , we further confirmed the specificity of the enzymatic reaction by transferring an aqueous buffer solution not containing an analyte , or with
bovine serum albumin (
BSA ) or
trypsin onto the interface of aqueous solutions and the
glyceryl trioleate - doped
5CB , which did not produce any distinctive contrast in the optical appearance . -
pubmed
Body weight , height ,
BMI , and waist circumference were recorded and fasting serum levels of
glucose ,
insulin ,
25 ( OH ) D , and
parathyroid hormone (
PTH ) were measured . -
pubmed
The mean level of
25 ( OH ) D was higher in the SGA group ( 26.2 + -10 vs. 17.2 + -7 ng / ml , p<0.01 ) but that of
PTH was no different . -
pubmed
In the SGA group ,
25 ( OH ) D was correlated with
HOMA - beta% but not with HOMA - IR or
insulin . -
pubmed
Using preclinical models and patients ' samples we recently reported that the emergence of
KRAS mutations but also
KRAS amplification is associated with acquired resistance to the
EGFR inhibitors
cetuximab or panitumumab . -
pubmed
Blockade of
MORs using 1 or 3 mug of
H - d - Phe - Cys - Tyr - d - Trp - Arg - Thr - Pen - Thr - NH2 within the dorsal striatum decreased mating during the cohabitation period and inhibited partner preference formation . -
pubmed
AIMS : While there is thought to be an association between
glucose and lipid metabolism , it is largely unknown whether
apolipoprotein B and non - high density lipoprotein (
HDL )
cholesterol are associated with the development of
Type 2 diabetes . -
pubmed
RESULTS :
Apolipoprotein B and non -
HDL cholesterol levels showed a strong association with the development of
Type 2 diabetes compared with conventional lipid measurements and their ratios [ hazard ratio per 1 sd ; 1.39 ( 95 % CI 1.37 - 1.42 ) and 1.38 ( 95 % CI 1.35 - 1.40 ) , respectively ; both P & lt ; 0.001 ] . -
pubmed
The Kaplan - Meier survival curve demonstrated that
Type 2 diabetes developed more frequently as
apolipoprotein B or non -
HDL cholesterol levels increased across quartiles ( both P & lt ; 0.001 ) . -
pubmed
In multivariate Cox regression analyses , both
apolipoprotein B and non -
HDL cholesterol were associated with the development of
Type 2 diabetes , independent of other risk factor including age , sex , waist circumference , family history of
diabetes , fasting serum
glucose and
insulin levels , HbA1c ,
systolic blood pressure and other conventional lipid measurements [ hazard ratio per 1 sd ; 1.14 ( 95 % CI 1.11 - 1.18 ) and 1.13 ( 95 % CI 1.10 - 1.16 ) , respectively ; both P & lt ; 0.001 ] . -
pubmed
Berberine protects against
palmitate - induced
endothelial dysfunction : involvements of upregulation of
AMPK and
eNOS and downregulation of
NOX4 . -
pubmed
The expressions of
eNOS were significantly increased , while
NOX4 protein expression was decreased in
berberine - treated HUVECs . -
pubmed
Moreover ,
berberine upregulated the protein expression of
AMPK and p -
AMPK in
palmitate - treated HUVECs , but had no effect on the levels of
Akt . -
pubmed
Therefore ,
berberine ameliorates
palmitate - induced
endothelial dysfunction by upregulating
eNOS expression and downregulating expression of
NOX4 . -
pubmed
This regulatory effect of
berberine may be related to the activation of
AMPK . -
pubmed
We determined the value of estimated
GFR ( eGFR ) as a threshold for the increase of plasma
cFGF23 ( C - terminal ) and intact
fibroblast growth factor-23 (
iFGF23 ) ( intact ) concentrations in the course of
chronic kidney disease (
CKD ) and compared this
eGFR value with values related to increased serum intact
parathyroid hormone (
iPTH ) or
phosphorus concentrations in an elderly population . -
pubmed
RESULTS : We found that
genistein treatment enhanced ECFCs ' migration and proliferation , which was accompanied by increases in the expression of
ILK ,
alpha - parvin ,
F - actin , and phospholylation of
ERK 1 / 2 signaling . -
pubmed
Transplantation of
genistein - stimulates ECFCs (
GS - ECFCs ) into myocardial ischemic sites in vivo induced cellular proliferation and secretion of angiogenic
cytokines at the ischemic sites and thereby enhanced neovascularization and decreased myocardial
fibrosis as well as improved cardiac function , as shown by echocardiography . -
pubmed
Furthermore ,
proline - rich antimicrobial peptides display the ability to not only modulate the immune system via
cytokine activity or angiogenesis but also possess properties of penetrating cell membranes and crossing the blood brain barrier suggesting a role as potential novel carriers . -
pubmed
Enzyme activity and transcript levels of
HEMA1 ,
GSA and
ALAD for
ALA synthesis greatly decreased in
ALA- and
OF - treated plants . -
pubmed
Transcript levels of
PPO1 ,
CHLH ,
CHLI , and
PORB genes involving
Mg -
porphyrin synthesis continuously decreased in
ALA- and
OF - treated plants , with greater decreases in
ALA - treated plants . -
pubmed
By contrast , up - regulation of
FC2 and
HO2 genes in
Fe - porphyrin branch was noticeable in
ALA and
OF - treated plants 1 day and 2 days after the treatments , respectively . -
pubmed
Decreased transcript levels of nuclear - encoded genes
Lhcb1 ,
Lhcb6 , and
RbcS were accompanied by disappearance of MgProto
IX in
ALA- and
OF - treated plants after 2 days of the treatments . -
pubmed
The up - regulation of
FC2 and
HO2 also appears to compensate for the photodynamic stress - induced damage . -
pubmed
Nigral and striatal redox balance , was studied by means of biochemical indicators such as
malondialdehyde (
MDA ) ,
nitric oxide (
NO ) and the
catalase enzymatic activity (
CAT EA ) . -
pubmed
Biochemical studies revealed that after 48 - h
PPN neurotoxic injury , the
CAT EA showed a significant increase in the subtantia nigra pars compacta ( SNpc ) ( p<0.05 ) . -
pubmed
This significant increase of
CAT EA persisted in the nigral tissue ( p<0.001 ) and reached the striatal tissue ( p<0.001 ) seven days after
PPN injury . -
pubmed
Also at seven days
post -
injury PPN , increased concentrations of
MDA ( p<0.01 ) and a tendency to decrease in the concentrations of
NO in both structures ( SNpc and striatum ) were found . -
pubmed
In particular , the role of
glycated hemoglobin (
HbA1c ) and oral
glucose tolerance testing ( OGTT ) will be reviewed . -
pubmed
BMP-1 digestion of
DSPP - derived proteins extracted from dental pulp did not generate new DPP bands on
sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( indicating an absence of intact
DSPP ) , although the results suggested another
BMP-1 cleavage site within
DSP . -
pubmed
Its
amino acid composition was similar to
DSP . -
pubmed
RESULTS :
Dabigatran altered the
prothrombin time , activated partial
thromboplastin time and
thrombin time , and the measurement of intrinsic and extrinsic factor levels . -
pubmed
Conversely , the addition of
dabigatran falsely corrected an abnormal activated
protein C resistance ratio . -
pubmed
MEASUREMENTS : Between 2011 and 2013 , fasting venous samples were analyzed for
25 ( OH ) D ,
iPTH ,
alkaline phosphatase (
ALP ) ,
calcium , and
phosphorus . -
pubmed
Several anti -
EGFR antibodies including
cetuximab are used in clinics for treatment of
EGFR - overexpressing
colorectal and head and neck cancers but the efficiency of these antibodies is threatened by their large size and chimeric nature . -
pubmed
Here , we aimed to define further the reactivity profile of CHB - associated antibodies towards
Ro52p200 (
amino acid 200 - 239 ) . -
pubmed
In particular ,
amino acid D233 appeared crucial to maternal antibody reactivity towards
p200 . -
pubmed
T Cells Encountering Myeloid Cells Programmed for
Amino Acid - dependent Immunosuppression Use
Rictor /
mTORC2 Protein for Proliferative Checkpoint Decisions . -
pubmed
Using a reduced complexity in vitro macrophage - T cell co - culture system , we show that
macrophage arginase-1 is the only factor required by M2 macrophages to block T cells in
G1 , and this effect is mediated by
l - arginine elimination rather than metabolite generation . -
pubmed
After 2 days of
arginine deprivation ,
mTORC1 activity declined paralleling a selective down - regulation of
SREBP target gene expression , whereas mRNAs involved in glycolysis , gluconeogenesis , and T cell activation were unaffected . -
pubmed
Arginine deprivation - induced cell cycle arrest was mediated in part by
Rictor /
mTORC2 , providing evidence that this nutrient recognition pathway is a central component of how T cells measure environmental
arginine . -
pubmed
For those receiving
atomoxetine , the summary pre -
post ( e.g. -
pubmed
The regulation of
tumor suppressor protein ,
p53 , and
estrogen receptor (
ERalpha ) by
resveratrol in
breast cancer cells . -
pubmed
Previous studies from our laboratory have shown the
tumor suppressor protein
p53 and
estrogen receptor - alpha (
ERalpha ) to be possible molecular targets for
RES . -
pubmed
In this study , the anti - estrogenic effects of
RES were analyzed on the expression of
ERalpha and
p53 . -
pubmed
Our studies indicate that
RES caused a decrease in the levels of protein expression of
p53 and
ERalpha as compared to the control . -
pubmed
RES , in conjunction with
ICI 182,780 ( ICI ) , caused a down - regulation of both
p53 and
ERalpha as compared to the control . -
pubmed
These observed effects on cell proliferation and regulation of both
p53 and
ERalpha by
RES may lead to further understanding of the relationship between
tumor suppressor proteins and
steroid receptors in
breast cancer cells . -
pubmed
AIM : The aim of this study was to compare the effectiveness of 0.1 %
levobupivacaine and 0.1 %
ropivacaine with
fentanyl as an adjuvant for epidural labor analgesia in terms of onset , duration , quality of analgesia , and degree of motor blockade . -
pubmed
Camptothecin resistance is determined by the regulation of
topoisomerase I degradation mediated by
ubiquitin proteasome pathway . -
pubmed
Proteasomal degradation of
topoisomerase I (
topoI ) is one of the most remarkable cellular phenomena observed in response to
camptothecin (
CPT ) . -
pubmed
Importantly , the rate of
topoI degradation is linked to
CPT resistance . -
pubmed
Here , we show that the
Ku70 /
Ku80 heterodimer binds with
topoI , and that the
DNA - dependent protein kinase (
DNA - PKcs ) phosphorylates
topoI on
serine 10 (
topoI -
pS10 ) , which is subsequently ubiquitinated by
BRCA1 . -
pubmed
A higher basal level of
topoI -
pS10 ensures rapid
topoI degradation leading to
CPT resistance . -
pubmed
Importantly ,
PTEN regulates
DNA - PKcs kinase activity in this pathway and
PTEN deletion ensures DNA -
PKcs dependent higher
topoI -
pS10 , rapid
topoI degradation and
CPT resistance . -
pubmed
Due to the color change property of
cobalt chloride , the as - spun composite NFMs show obviously macroscopic color change from blue to pink as relative humidity (
RH ) increasing from 12.4 to 97.2 % . -
pubmed
Owing to the above double sensitive mechanism on
RH , the
PA66 /
CoCl2 composite
NFM may show great potential applications from meticulous to coarse . -
pubmed
Batch experiments showed that optimal
K2FeO4 dosage and reaction time for sludge disintegration was 100mg / g suspended solids (
SS ) and 24h , respectively . -
pubmed
Sgp130 , a natural antagonist of
IL-6 trans - signaling , and sex hormones , especially
testosterone and
estradiol , are prominently considered as anti -
atherosclerotic . -
pubmed
Serum concentrations of
hs - CRP ,
testosterone ,
estradiol , and other routine biochemical markers were also quantified . -
pubmed
Besides , we observed the effects of
testosterone and
estradiol on
sgp130 in HUVECs . -
pubmed
In addition , multiple regression analysis indicated that serum
sgp130 was positively associated with
estradiol ( beta - coefficient = 0.450 , p & lt ; 0.001 ) and T /
E2 ratio ( beta - coefficient = 0.257 , p = 0.001 ) . -
pubmed
Furthermore , basic studies found that supernatant
sgp130 levels of HUVECs were significantly increased by the cooperativity of
testosterone and
estradiol . -
pubmed
ECM were extracted with
methanol , precipitates were separated by centrifugation and metabolite production of individual embryo was analysed by LC -
MS ( the positive ion mode ) . -
pubmed
Changes in urinary
albumin excretion rate ( UAER ) , serum
malondialdehyde (
MDA ) ,
superoxide dismutase (
SOD ) activity , urine
8 - hydroxyl deoxyguanosine (
8 - OHdG ) , serum
creatinine , estimated
glomerular filtration rate , and lipid profile before and after 24 weeks of follow - up were examined and compared . -
pubmed
SOD activity decreased significantly after 24 weeks in the
SCH group ( p & lt ; 0.05 ) . -
pubmed
The levels of mutant
K - RAS and mutant
N - RAS are rapidly reduced in a
Beclin1 /
ATG5 -dependent fashion by the irreversible
ERBB1 / 2 / 4 inhibitor
neratinib . -
pubmed
The FDA approved irreversible inhibitor of
ERBB1 / 2 / 4 ,
neratinib , was recently shown to rapidly down - regulate the expression of
ERBB1 / 2 / 4 as well as the levels of
c - MET and mutant
K - RAS via autophagic degradation . -
pubmed
In the present studies , in a dose - dependent fashion ,
neratinib reduced the expression levels of mutant
K - RAS or of mutant
N - RAS , which was augmented in an additive to greater than additive fashion by the
HDAC inhibitors
sodium valproate and
AR42 . -
pubmed
Neratinib could reduce
PDGFRalpha levels in
GBM cells , that was enhanced by
sodium valproate . -
pubmed
Knock down of
Beclin1 or of
ATG5 prevented
neratinib and
neratinib combined with
sodium valproate /
AR42 from reducing the expression of mutant
N - RAS in established
PDX and fresh
PDX models of
ovarian cancer and
melanoma , respectively . -
pubmed
Neratinib and the drug combinations caused the co - localization of mutant
RAS proteins and
ERBB2 with
Beclin1 and
cathepsin B. -
pubmed
Collectively , our data reinforce the concept that the irreversible
ERBB1 / 2 / 4 inhibitor
neratinib has the potential for use in the treatment of
tumors expressing mutant
RAS proteins . -
pubmed
The structure , dynamics , and
ozone signal of the Quasi - Biennial Oscillation produced by the 35 - year NASA MERRA-2 ( Modern -
Era Retrospective Analysis for Research and Applications ) reanalysis are examined based on monthly mean output . -
pubmed
AIM AND OBJECTIVE : The multi - component condensation of
benzil ,
primary amines ,
ammonium acetate and various
aldehydes was efficiently catalyzed using
cobalt oxide nanoparticles under ultrasonic irradiation . -
pubmed
In addition , the catalytic influence of the nanocatalyst was examined in the one -
pot reaction of
primary amines ,
benzil ,
ammonium acetate and diverse
aromatic aldehydes under ultrasonic irradiation . -
pubmed
Discovery of
MK-8282 as a Potent
G - Protein - Coupled Receptor 119 Agonist for the Treatment of
Type 2 Diabetes . -
pubmed
G - Protein coupled receptor 119 (
GPR119 ) is a
GPCR target at which agonists have demonstrated
glucose - dependent
insulin secretion and shows beneficial effects on glycemic control . -
pubmed
The overall aim of this study was to evaluate whether
iodine-131 radiolabeled monoclonal antibody ( mAb ) targeting
programmed death - ligand 1 (
PD - L1 ) can be used for imaging of
PD - L1 expression noninvasively in vivo and playing synergistic effect combined with immunotherapy . -
pubmed
Anti -
PD - L1 mAb was radiolabeled with
iodine-131 ( 131 I -
PD - L1 mAb ) and was characterized in vitro . -
pubmed
Fluoride - Releasing Effect of a Modified Resin Denture Containing S -
PRG Fillers on Salivary
Fluoride Retention : A Randomized Clinical Study . -
pubmed
This study aimed to evaluate the clinical effect of a resin denture base containing 20wt% surface prereacted glass ionomer ( S -
PRG ) fillers on saliva
fluoride concentrations as a surrogate evaluation of
caries control . -
pubmed
A significant increase in salivary
fluoride concentration in the S -
PRG denture group was observed from baseline to day 1 , followed by a significant decrease to an approximate baseline level . -
pubmed
The resin denture base containing 20wt% S -
PRG fillers demonstrated an initial
fluoride release that increased saliva
fluoride concentrations , but the
fluoride release from within the material was short lived . -
pubmed
Intense near - infrared ( NIR ) emitting phosphors
Ca5 ( PO4 ) 3 Cl :
Eu2 + ,
Nd3 + were synthesized using a conventional solid - state reaction method and characterized using X - ray diffraction , reflectance , photoluminescence ( PL ) emission and
PL excitation ( PLE ) spectroscopy , and
PL lifetime measurements . -
pubmed
We compared the
AMH levels of the two groups and receiver operating characteristic ( ROC ) curve was used to determine the threshold for
AMH in predicting the ovulatory response to
CC therapy . -
pubmed
AMH values and AFC were found to be predictors of the adequate ovulatory response to
CC . -
pubmed
Protocorm - like body (
PLB ) segments formed calli in Vacin and Went medium with
sucrose . -
pubmed
Paperless ophthalmology units are feasible in
today 's
NHS and can produce near complete
cataract data sets ; this can ultimately lead to more comprehensive and reliable aggregate
cataract outcome data . -
pubmed
For the evaluation of osteogenic differentiation ,
alkaline phosphatase (
ALP ) activity and staining ,
Alizarin red staining , and a quantitative real - time
polymerase chain reaction ( RT - PCR ) analysis were performed . -
pubmed
The increased level of
ALP production , deposition of
calcium mineralization , as well as the expression of typical osteogenic genes , indicated the enhancement of osteogenic differentiation of hMSCs . -
pubmed
Moreover , the incorporation of
Au could activate the
Wnt /
beta - catenin signaling pathway , which seemed to be the molecular mechanism underlying the osteoinductive capability of HA -
Au nanoparticles . -
pubmed
Furthermore ,
TAZ regulates the expression of
Epithelial Membrane Protein 1 (
EMP1 ) , which , in turn , induces the expression of
nicotinamide adenine dinucleotide phosphate ( NADPH ) Oxidase 4 (
NOX4 ) , a renal - enriched reactive
oxygen species ( ROS)-generating enzyme essential for ferroptosis . -
pubmed
ISR /
ISO was associated with age ( p = 0.01 ) and
CRP level > 5 mg / l ( p = 0.043 ) , while greater stent length was associated with ISR only in the
DES group ( p = 0.024 ) . -
pubmed
Herein , we report a new multifunctional supramolecular eutectic liquid ( SEL ) binder , consisting of
diphenylamine (
DP ) and
benzophenone ( BP ) , which enables a strongly improved conductive network and anchored
polysulfides for high - performance
lithium -
sulfur batteries . -
pubmed
Airway inflammation ( assessed by exhaled
nitric oxide ( eNO ) ) increases after a single high - fat meal (
HFM ) , yet this response may be modified by airway stretch and baseline eNO level . -
pubmed
The results showed that after WAS was treated by 20 mg / g total suspended solids ( TSS )
Fe ( II ) and 50 mg / g TSS SPC , the water content of sludge cake ( WCSC ) by press filtration and capillary suction time (
CST ) dropped from 90.8 % + - 1.6 % and 96.1 + - 4.0 s ( the control ) to 55.6 % + - 1.4 % and 30.1 + - 2.5 s , respectively . -
pubmed
Biophysical study of
phloretin with
human serum albumin in liposomes using spectroscopic methods . -
pubmed
In this study , the interaction between
phloretin and
human serum albumin (
HSA ) in an L - egg lecithin
phosphatidylcholine ( PC ) liposome suspension was investigated by fluorescence and absorbance spectroscopy . -
pubmed
The results show that
phloretin efficiently quenches the intrinsic fluorescence of
HSA through a combination of dynamic and static quenching . -
pubmed
The values of Gibbs free energy , and the enthalpy and entropic change in the binding process of
phloretin with
HSA in the PC liposome suspensions were negative , suggesting that the binding process of
phloretin and
HSA was spontaneous . -
pubmed
In addition , binding of
phloretin to
HSA in liposome suspensions was investigated by synchronous fluorescence spectroscopy . -
pubmed
The pooled standard mean difference ( 95 % confidence interval ) between exposed and unexposed groups was estimated as of 0.74 ( 0.28 , 1.21 ) for
alkaline phosphatase (
ALP ) , 2.44 ( 1.80 , 3.08 ) for
aspartate transaminase (
AST ) , 2.06 ( 1.42 , 2.69 ) for
alanine transaminase (
ALT ) , 0.10 ( -0.09 , 0.29 ) for total
Bilirubin ( TB ) , 0.74 ( -0.42 , -1.90 ) for
total protein (
TP ) , -0.49 ( -0.82 , -0.15 ) for
albumin , 0.88 ( -0.10 , 1.87 ) for
uric acid , 1.02 ( 0.45 , 1.59 ) for
creatinine and 1.44 ( 0.75 , 2.13 ) for blood
urea nitrogen (
BUN ) . -
pubmed
The metal - ion activator ,
Mg2 + , did not affect the equilibrium between
nucleotide and
enzyme . -
pubmed
The beginning of this plateau depended on both the
albumin preparation and the
Polybrene lot . -
pubmed
Modulation of
glucose metabolic response to endotoxin by
granulocyte colony - stimulating factor . -
pubmed
The present study examines whether in vivo administration of
granulocyte colony - stimulating factor (
G - CSF ) and the resultant
neutrophilia alters basal
glucose metabolism or modulates the
glucose metabolic response to a subsequent endotoxin [ lipopolysaccharide ( LPS ) ] challenge . -
pubmed
The elevated rate of whole body
glucose utilization was attributable to the
G - CSF - enhanced LPS - induced increase in
glucose uptake by the ileum , spleen , liver , and lung . -
pubmed
While basal enzyme activity was not altered ,
galanin from 10(-11 ) M to 5 x 10(-7 ) M decreased
forskolin- and
VIP - stimulated
adenylate cyclase with a half - maximal effect being elicited by 0.7 nM
neuropeptide and a maximal 80 % inhibition of the enzyme activity . -
pubmed
The
galanin fragments ( 2 - 29 ) and ( 1 - 15 ) dose - dependently inhibited the
forskolin - stimulated
adenylate cyclase , while the fragments ( 3 - 29 ) and ( 10 - 29 ) were found inactive . -
pubmed
The potency of the relaxation induced by 10(-9 ) M of
ET-1 was 16.0 + /- 1.3 % , compared to the response to
isoproterenol ( 2 x 10(-5 ) M ) , and the duration of the relaxation was 7.2 + /- 0.7 minutes . -
pubmed
[ Fluorometric determination of
monoaminoxidase activity with
1 - methyl-4 - phenyl-1,2,3,6 - tetrahydropyridine as substrate ] . -
pubmed
We studied
thrombin - induced changes in cytosolic
calcium concentration (
Ca2 + i ) using microfluorometry of
fura-2 - loaded single cells , cell topography ( scanning electron microscopy ) , and cytoskeleton (
rhodamine phalloidin ) in endothelial cells . -
pubmed
Thrombin caused an initial and sustained phase of an increase in
Ca2 + i . -
pubmed
Pretreatment with
pertussis toxin abolished both phases of
Ca2 +i response . -
pubmed
Sustained phase of
thrombin effect required extracellular
calcium . -
pubmed
Pretreatment of endothelial cells with
indomethacin protracted the sustained phase , whereas a
lipoxygenase inhibitor ,
nordihydroguaiaretic acid curtailed it . -
pubmed
Thrombin caused a marked retraction of confluent endothelial cells coincident with the sustained phase of
Ca2 +i response . -
pubmed
Pretreatment of endothelial cells with
nordihydroguaiaretic acid blunted the
thrombin - induced cell retraction . -
pubmed
The sustained response required microinjection of
phospholipase A2 or co - injection of
phospholipase A2 with
phosphatidylinositol 4,5 - bisphosphate - specific
phospholipase C ,
phosphatidylinositol 1,4,5 - trisphosphate , or
CaCl2 , further implying that
thrombin receptor(s ) can be coupled to both
phospholipases C and A2 . -
pubmed
Sustained elevation of
Ca2 + i was a necessary prerequisite for the
thrombin - induced changes in endothelial cell topography . -
pubmed
The biological activity and heparin binding ability was retained when the
serine was substituted for the
cysteine residue at either 70 or 88 of the
bFGF protein . -
pubmed
The substitution of the residues at these positions , especially at position 88 , reduced the heterogeneity recognized as several peaks of
bFGF eluted from a heparin affinity column , even after oxidation with
hydrogen peroxide , suggesting that the
cysteines at these positions are exposed to the surface of the molecule to form
disulfide bonds that induce heterologous conformations . -
pubmed
In this study the dynamic contrast behavior after
Gd - DTPA of different breast tissues and
tumors has been investigated with a series of
T1 - weighted FLASH - sequences during the first 5 minutes after the application of
Gd - DTPA . -
pubmed
DD between
carcinomas and
proliferative dysplasia ) was possible on FLASH - scans early after
contrast medium than on the late
SE - scans . -
pubmed
,
dipeptidylpeptidase II (
DPP II ) reaction was carried out in smears of peripheral blood using
Lys - Pro - MNA and
Lys - Ala - MNA as substrates and
Fast Blue B ( FBB ) as the coupling agent in
cacodylate buffer , pH 5.5 . -
pubmed
Inactivation of
sulfatase A by
calomel electrodes . -
pubmed
Hemoglobin F - Caltech : alpha 2 G gamma 2 120Lys replaced by
Gln . -
pubmed
In in vitro experiments , the inactivation of
rheumatoid factor and the inhibition of
collagenase and
bone alkaline phosphatase activities were observed with both compounds , but these effects of
SA96 were more potent than those of
D - PA . -
pubmed
Within 10 sec of the addition of
TRH ( 1 microM ) , there was a maximal 60 % decrease in
PtdIns 4,5P2 and 40 % decrease in PtdIns 4P. -
pubmed
This early rise in
1,2 - diacylglycerol levels coincided in time and was equivalent in lipid mass with the decrease in the
polyphosphoinositide content , suggesting the involvement of a
phospholipase C - type enzyme . -
pubmed
1,2 - Diacylglycerol levels subsequently fell toward control values and , after 3 min of treatment with
TRH , rose again to levels 50 % above normal . -
pubmed
These results suggest that
TRH stimulates a cycle of events in which the breakdown of the
polyphosphoinositides ,
PtdIns 4,5P2 and , perhaps ,
PtdIns 4P by a
phospholipase C enzyme could be the initiating event . -
pubmed
Reaction of isolated subunits with the
dialdehyde derivative of
ADP is slow compared to the initial reaction with native
enzyme , indicating differences in the effects of
ADP on intact enzyme and subunits . -
pubmed
Long - term beneficial effects of
endralazine , a new arteriolar vasodilator at
rest and during exercise capacity in chronic
congestive heart failure . -
pubmed
Pulsatile secretion of
oestradiol was observed at frequencies similar to
LH during the periods 4 - 12 h ( 3 heifers ) and 24 - 36 h ( 2 heifers ) after PG , respectively , resulting in higher ( P less than 0.05 ) mean
oestradiol concentrations . -
pubmed
ASA and even
SA blocked the effects of
bradykinin on both parameters . -
pubmed
While
SA had no effect on
LTC4 ,
ASA blocked the effects of
LTC4 upon both tracheal pressure and nerve activity . -
pubmed
These results suggest that both
LTC4 and
bradykinin may act through mediators derived from
arachidonic acid not only in increasing tracheal pressures but also to increase
RAR nerve activity . -
pubmed
In addition , using
bovine serum albumin or the synthetic polymer
Kollidone , inhibition could be prevented as well as abolished . -
pubmed
The reaction includes
neuraminidase hydrolysis of
glycoprotein , cleavage of
sialic acid to
pyruvate by
N - acetyl neuraminic acid ( NANA )- aldolase , oxidation of
pyruvate by
pyruvate oxidase which produces
hydrogen peroxide , and colorimetry of
hydrogen peroxide using the
peroxidase -
p - chlorophenol-4 - amino - antipyrine method . -
pubmed
Although the disease continued to progress during
interferon treatment , a change to a regime of
deoxycoformycin resulted in an excellent response . -
pubmed
Fas antigen (
Ag ) was expressed in various thyroid tissues , including normal subjects . -
pubmed
Tyrosine at position 26 in the second hypervariable region in the
beta 1 domain of the DQB1 gene is common to these two alleles and is not present in any other known
DQB1 alleles . -
pubmed
One major B cell epitope , located within the region corresponding to
amino acid residues 74 - 248 , was perfectly associated with the
amino acid sequence FLEDR at positions 67 - 71 in the
beta 1 domain of the DRB gene . -
pubmed
Two other epitopes , corresponding to 316 - 441 or 658 - 700 , were associated with the serologically defined
HLA - DR52 antigen . -
pubmed
Pretreatment with
indomethacin did not significantly alter the effects of
guanylin and
STa . -
pubmed
The levels of three OLS , namely
leucine - enkephalin (
LEK ) ,
beta - endorphin (
beta - EP ) and
dynorphin A1 - 13 ( DynoA1 - 13 ) of 44 cases with
neonatal asphyxia were studied by radioimmunoassay . -
pubmed
The OLS levels of
CSF were also higher in cases with
cerebral injury than in those without
cerebral injury , while the levels of
OLS in plasma had no difference in these two groups . -
pubmed
This receptor demonstrated a rank order of potency for muscarinic antagonist binding characteristic for the
M3 receptor : with high affinity binding for
hexahydrosiladifenidol , Kd : 16 + /- 2 nM ( + /- S.E. -
pubmed
Moderate stimulation of contractility by
isoprenaline ( 0.01 mumol / L ) partly reversed the negative force - frequency relationship in NYHA class
IV and preserved the positive force - frequency relationship in nonfailing myocardium . -
pubmed
The addition of
ouabain and
BDF 9148 together restored completely the force - frequency relationship in NYHA class
IV . -
pubmed
The resulting
H-2Ld / Q10d protein is homologous to the
native H-2Ld heavy chain for the three external domains except for an
Arg to
His substitution at position 260 . -
pubmed
This molecule reacts with
H-2Ld - specific mAb that identify determinants on the first and second domains as well as with an anti - Q10
carboxyl - terminal peptide antiserum , but is not recognized by a mAb specific for a determinant of
H-2Ld third domain . -
pubmed
The integrity of antibody reactivity of the first and second domains together with
beta 2 - microglobulin association suggest that our molecule may be considered a good soluble counterpart of the native membrane
H-2Ld molecule with which to perform functional studies . -
pubmed
The
enzyme activity of both domains was completely inactivated in the presence of
copper or
silver ions and partially inactivated by
iron or
zinc ions . -
pubmed
Serum
alkaline phosphatase activity and the urinary
hydroxyproline /
creatinine ratio were quickly and drastically reduced in all three groups . -
pubmed
A significant reduction of
serum alkaline phosphatases and the
hydroxyproline /
creatinine ratio was still present six months after the five day therapeutic course , reflecting a sustained activity of
tiludronate even after stopping treatment . -
pubmed
During the operation , supplemental ( rescue ) medication consisted of
fentanyl 25 micrograms
IV , bolus injections in all four treatment groups . -
pubmed
Therefore , we evaluated effects of
SH on plasma nutrient and pancreatic hormone (
insulin ,
glucagon ) concentrations . -
pubmed
Previous procedures were followed except dietary
carbohydrate was standardized to
glucose polymers for all three diets , and sampling was extended to 3 h. Overall , portal or arterial nutrient and hormone concentrations were not different in the
SI and SH groups ( P & gt ; 0.05 ) , indicating that hydrolyzed soy protein did not cause abnormal plasma concentrations . -
pubmed
The overall mean ( SD )
urea clearance was 26.6 ( 6.0 ) ml.min-1 and the overall
creatinine clearance was 30.1 (
6.3 ) ml.min-1 . -
pubmed
Creatinine clearance was significantly lower in filters that failed within 24 h ( filters & lt ; 24 h 27.5 (
6.3 ) ml.min-1 ; filters & gt ; 24 h 32.2 ( 5.5 ) ml.min-1 ) . -
pubmed
Purified
rGal was homogeneous as judged by
SDS - PAGE analysis with the relative molecular mass under reducing conditions of 39.8 , and under nonreducing conditions 38.0 kDa . -
pubmed
Serum
cortisol levels and their diurnal variations ,
ACTH and 24 - h urinary
17 - ketosteroids ,
ketogenic steroids ,
17 - hydroxy steroids , and
creatinine , were measured . -
pubmed
Ion - dependent inactivation of
barium current through
L - type calcium channels . -
pubmed
These observations suggest that activation of a novel
staurosporine - sensitive
protein kinase is necessary for basal expression of the
preproAM gene in these cells . -
pubmed
The profiles of
SF extracts of aqueous homogenates of ramp bulbs from three different seasons and growing regions revealed that the
thiosulfinates were major components . -
pubmed
It recognizes a
carbohydrate antigen (
Ley ) which is expressed in a variety of
solid tumors including breast and colon . -
pubmed
Pre - incubation of cell cultures with
cholera toxin significantly down - regulated lipopolysaccharide - induced
NO synthesis and
phorbol-12 - myristate-13 - acetate - induced respiratory burst . -
pubmed
Analysis of a
phenobarbital - responsive enhancer sequence located in the 5 ' flanking region of the
chicken CYP2H1 gene : identification and characterization of functional protein - binding sites . -
pubmed
We previously identified in the
chicken CYP2H1 gene an upstream enhancer domain ( -5900/-1100 ) that responds to
phenobarbital . -
pubmed
Melittin rapidly quenched the isotropic signal in the
EPR spectrum of spin - labeled
stearic acid added to
SR membranes . -
pubmed
It was demonstrated with the use of a cross - linking agent ,
cupric o - phenanthroline , that melittin induced slow aggregation of
Ca - ATPase protein in SR membranes ; the content of
enzyme in the monomeric form decreased with rate constant 0.14 min-1 . -
pubmed
Furthermore , IGF-1 facilitates
glucose metabolism , lowers
insulin levels , increases
insulin sensitivity , and improves the lipid profile . -
pubmed
In the brain the
renin -
angiotensin system has been implicated in the regulation of the cardiovascular system as well as of regulation of reproductive hormones , water balance and behavior . -
pubmed
This biologically active
Ang II 3 - 8 fragment is referred to as
angiotensin IV (
Ang IV ) and has been reported to participate in memory acquisition and recall as well as in the regulation of blood flow , neurite outgrowth , angiogenesis and kidney function . -
pubmed
Its tryptic peptide mass profile was determined using the same technique , and the
amino acid sequence of two internal peptides was obtained by tandem
MS and Edman degradation . -
pubmed
The two enzymes showed the highest affinity for
angiotensin III ( Ki & lt ; 1 microM ) and a preference for ahydrophobic residue at the
P1 ' site . -
pubmed
A highly enantioselective methodology for the synthesis of the
GABA ( B ) receptor agonist
( R )-(-)- baclofen is described . -
pubmed
Crystals have been obtained using
PEG 8000 as precipitant and
cacodylate at pH
6.3 as buffer . -
pubmed
Adding ten per cent
beta - cyclodextrin , a
cyclic oligosaccharide that binds
cholesterol and
bile acids to the lithogenic diet at the expense of
sucrose , induced a decrease in
cholesterol bile secretion and in the CSI and
HI and prevented
cholesterol gallstone formation . -
pubmed
Efficient
( 13 ) C labeling was achieved by blocking the endogenous pathway genetically or chemically during the feed of a
( 13 ) C labeled precursor specific to the
MEP or
MVA pathways . -
pubmed
Liquid chromatography - mass spectrometry analysis demonstrated that the
prenyl group of
trans - zeatin (
tZ ) and
isopentenyladenine is mainly produced through the
MEP pathway . -
pubmed
In comparison , a large fraction of the
prenyl group of
cis - zeatin (
cZ ) derivatives was provided by the
MVA pathway . -
pubmed
Organotin derivatives were measured in both the visceral
coil and the
rest of the soft body of the organisms . -
pubmed
in the visceral
coil , and from 96 + /- 24 to 297 + /- 107 ng
Sn g(-1 ) d.w . -
pubmed
( visceral
coil ) and from 0.25 to 32 + /- 22 ng
Sn g(-1 ) d.w . -
pubmed
Recombinant factor VIIa ( NovoSeven ) [ also known as
recombinant activated factor VII or eptacog alfa ] is a
vitamin K - dependent glycoprotein that is structurally similar to
human plasma - derived factor VIIa . -
pubmed
In the
BPI group , there were significantly more nulliparous women , with more use of
oxytocin . -
pubmed
Pharmacokinetics and pharmacodynamics of
etizolam are influenced by polymorphic
CYP2C19 activity . -
pubmed
OBJECTIVE : To examine the effect of
cytochrome P450 ( CYP ) 2C19 activity on the single - dose pharmacokinetics and pharmacodynamics of
etizolam . -
pubmed
CONCLUSION : The present study suggests that the single - dose pharmacokinetics and pharmacodynamics of
etizolam are influenced by polymorphic
CYP2C19 activity . -
pubmed
Data was compared between the 2 acquisition methods at
rest and between the single breath - hold acquisition at
rest and during
adenosine stress . -
pubmed
Herein , ab initio density functional theory is used to study the thermal decomposition of the
Keggin molecular
HPA structure through the desorption of constitutional water molecules . -
pubmed
The overall reaction energy and activation barrier are computed for the overall reaction
HnXM12O40 - ->
Hn-2XM12O39+
H2O. and subsequently used to predict the effect of
HPA composition on thermal stability . -
pubmed
We designed and validated the real - time quantitative
polymerase chain reaction ( Q - PCR ) assays with
SYBR Green dyes for simultaneous quantification of the expression of the 16 genes . -
pubmed
Potassium permanganate footprinting on the nontemplate and template strands indicates that when
polymerase is in a stable ( open ) complex with P(minor ) , the DNA is single stranded from positions -11 to +4 . -
pubmed
Co - treatment with
EGCG and the protein synthesis inhibitor
cycloheximide prominently shortened Bad half - life , with as little as 30 % of the
Bad protein content remaining after 2 h , suggesting an effect of
EGCG on
Bad protein degradation . -
pubmed
Accordingly , the proteasome inhibitors
MG-132 and
lactacystin damped
Bad down - regulation by
EGCG . -
pubmed
The general
protein kinase C (
PKC ) inhibitor
GF109203X , or the down - regulation of conventional and novel
PKC isoforms , abolished
EGCG - induced
Bad decline . -
pubmed
The enforced expression of
Bad for up to 72 h rendered the cells more susceptible to serum deprivation - induced cell
death , whereas
EGCG treatment significantly improved cell viability ( up to 1.6 - fold ) . -
pubmed
The present study reveals a novel pathway in the neuroprotective mechanism of the action of
EGCG , which involves a rapid
PKC - mediated degradation of
Bad by the proteasome . -
pubmed
Functional significance of
Asn - linked glycosylation of
proteinase 3 for enzymatic activity , processing , targeting , and recognition by anti - neutrophil cytoplasmic antibodies . -
pubmed
PR3 has two potential
Asn - linked glycosylation sites . -
pubmed
Using glycosylation deficient
rPR3 mutants we found that glycosylation at
Asn-147 , but not at
Asn-102 , is critical for thermal stability , and for optimal hydrolytic activity of
PR3 . -
pubmed
Efficient
amino - terminal proteolytic processing of
rPR3 is dependent on glycosylation at
Asn-102 . -
pubmed
AIM : To evaluate and compare the clinical outcomes of prophylactic submucosal saline -
epinephrine injection and saline injection alone for large colon
polyps by conventional polypectomy . -
pubmed
Surface properties of ultrathin films of cellulose esters deposited onto
silicon wafers have been investigated by means of contact angle measurements and atomic force microscopy (
AFM ) . -
pubmed
Only
CAB films with lower degree of esterification presented dewetting ,
CAB films with high degree of
butyrate were stable even after one week annealing . -
pubmed
Negative values of A(eff ) were found for CA ,
CAP , and lower
butyrate content
CAB , which are related to instability and agree with dewetting phenomena observed by
AFM . -
pubmed
In contrast , a positive A(eff ) was determined for higher
butyrate content
CAB , corroborating with experimental observations . -
pubmed
The mean intensity in grayscale units ( 0 - 255 ) of anti -
gentamicin labeling for type I hair cells was 28.14 ( 95 % CI 24.60 - 31.69 ) , for type
II hair cells was 17.09 ( 14.99 - 19.20 ) , and for support cells was 5.35 ( 5.34 - 5.46 ; p & lt ; 0.001 , ANOVA ) . -
pubmed
Intensity of fluorescence with
Texas - Red conjugated
gentamicin was 25.38 ( 22.83 - 27.94 ) in type I hair cells , 15.60 ( 14.73 - 16.48 ) in type
II cells , and 12.62 ( 12.06 - 13.17 ) in support cells ( p & lt ; 0.001 , ANOVA ) . -
pubmed
In addition ,
zolpidem ( 100 nm ) , a modulator of alpha(1 ) subunit - containing
GABA ( A ) receptors , selectively affected mIPSC(S ) suggesting that different postsynaptic
GABA ( A ) receptors mediate mIPSC(F ) and mIPSC(S ) . -
pubmed
As
CGP55845 , a
GABA ( B ) receptor antagonist , eliminated the observed difference in PPR , the lower release probability at IPSC(F ) connections probably reflects a stronger tonic
GABA ( B ) receptor - mediated inhibition of IPSC(F ) synapses . -
pubmed
Thus ,
CR cells receive two GABAergic inputs , which differ in the quantal amplitude , the probability of
GABA release and the frequency dependence of signal transfer . -
pubmed
Calcimimetics drug such as
cinacalcet suppress the secretion of
parathyroid hormone by sensitizing
parathyroid calcium receptors to extracellular ionized
calcium . -
pubmed
The influence of a new parenteral lipid emulsion (
LE ) containing
fish oil ( SMOF ) was experimentally evaluated on neutrophils ' chemotaxis and macrophages ' phagocytosis . -
pubmed
Only
MCT /
LCT / FO increased the number of spleen resident macrophages that had engaged in phagocytosis ( p<0.05 ) . -
pubmed
SMOF
LE had neutral effect while
fish oil LE enriched with MCT / LCT LE increased resident - macrophages ' phagocytosis . -
pubmed
Ganglioside GM1 - binding sites in
interleukin-4 : a photoaffinity labeling study . -
pubmed
Plasma
cortisol concentration was increased during
hypercapnia , and administration of exogenous
cortisol caused a marked increase in
NHE3 mRNA and protein . -
pubmed
Thus we speculate that the elevation of plasma
cortisol during
hypercapnia contributes to transcriptional activation of
NHE3 that ultimately promotes acid - base regulation by stimulating
H (+ ) secretion and
HCO ( 3 )(- ) reabsorption . -
pubmed
The
resveratrol analogue
3,5,3 ' , 4 ' , 5 '- pentahydroxy - trans - stilbene inhibits cell transformation via
MEK . -
pubmed
Further , we identified
MEK /
ERK signaling as the direct molecular target for the anticancer effects of
RSVL2 and demonstrated that
RSVL2 inhibited
MEK1 , but not
Raf1 or
ERK2 kinase activity . -
pubmed
RSVL2 also dose - dependently suppressed
MEK1 kinase activity induced by
TPA and the inhibition of
H - Ras - induced cell transformation was much stronger for RSVL2 than for
PD098059 or
resveratrol . -
pubmed
Both in vitro and ex vivo pull - down assays indicated that
RSVL2 , but not
resveratrol , directly bound with
GST -
MEK1 , but did not compete with
ATP for binding . -
pubmed
Fasting blood samples were analyzed for
glucose ,
HbA1 - c ,
insulin ,
C - peptide ,
leptin and lipid profile . -
pubmed
RESULTS : Mean serum levels of
glucose ( fasting and following 2h - OGTT ) ,
C - peptide and
leptin in male offspring of
diabetic parents were higher than in male offspring of NDP . -
pubmed
Oxorhenium - mediated cyclization and self - assembly of peptides : a combinatorial approach for the selection of new ligands of the
human cyclophilin hCyp-18 . -
pubmed
Vasopressin (
VP ) is typically reserved as a second - line agent due to the concern of
ischemia . -
pubmed
CONCLUSIONS : Results indicate that DB is ineffective in increasing
CO or improving mesenteric blood flow when used with physiologic replacement doses of
VP . -
pubmed
Addition of
aspartate increased acid survival of the wild type but not the
aspA knockout mutant . -
pubmed
AspA increases acid survival by producing
ammonia as demonstrated by mutation and in vitro enzyme activity analyses . -
pubmed
Calcineurin inhibitors such as
cyclosporin A (
CsA ) are the mainstay of immunosuppressive treatment for organ transplant recipients . -
pubmed
The proportion of
SON to total soluble
nitrogen (
TSN ) in 0 - 20 cm soil layer was 79.17 - 80.78 % for
CAC , 68.64%-74.51 % for CUL , and 59.97%-69.66 % for ALG . -
pubmed
With increasing soil depth , the proportion of soil SON to soil TSN and total
nitrogen (
TN ) for the three forest stands all increased . -
pubmed
Soil
SON content had a significant positive correlation with soil organic
carbon ( r=0.982 , P<0.01 ) , and also , had definite correlations with soil
TSN ,
TN ,
NH4 (+)- N , and
NO3 (-)- N. -
pubmed
The testes , male accessory glands ( MAGs ) , ovaries , and CA contained the long - chain intermediates in the
JH biosynthetic pathway ,
farnesoic acid (
FA ) ,
methyl farnesoate ( MF ) , and
JH III . -
pubmed
The testes and ovaries , but not CA , produced radioactive
JH III after the addition of
( 3 ) H - methionine and , separately , unlabeled
methionine , to the incubation medium . -
pubmed
Addition of
FA to incubation medium yielded increased
JH III , again indicating methylation of
FA to MF in the testes , MAGs , ovaries , but not CA . -
pubmed
germari proceeds via oxidation to
FA , methylation to MF , and epoxidation to
JH III . -
pubmed
The results indicated that the frequently of G allele and
Ile /
Val +
Val /
Val of
GSTP1 gene ( 26.7%and 44 % respectively ) in
AL group were higher than those in control group ( 10 % and 16 % respectively ) ; the
AL risk for persons with
Ile /
Val +
Val /
Val was 3.260 - fold ( 95%CI = 1.527 - 5.236 ) of persons with
Ile /
Ile . -
pubmed
Combined genotype analysis showed that the
AML risk for persons in combination of lle /
Val +
Val /
Val of
GSTP1 gene with C1C2 + C2C2 of
CYP2E1 gene increased by 3.208 - fold . -
pubmed
It is concluded that the
GSTP1 gene is related with susceptibility to
AML , the
AL risk for persons with lle /
Val +
Val /
Val of
GSTP1 gene decreased , while
CYP2E1 gene is not related with susceptibility to
AL , the
AML risk for persons in combination of
GSTP1 wildtype with
CYP2E1 hybrid and mutant genotype can be further decreased . -
pubmed
Furthermore ,
estrogen receptor 1 mRNA is over - expressed in early - stage
tumors , suggesting that expression of the oviduct - related genes may be regulated by
estrogen . -
pubmed
Oxidized and nitrated
oleic acid in biological systems : analysis by
GC -
MS /
MS and LC -
MS /
MS , and biological significance . -
pubmed
cis - EpOA is formed from
oleic acid by the catalytic action of various
cytochrome P450 isozymes . -
pubmed
The sensitivity and specificity of the
MS /
MS approach make electron - capture negative ion chemical ionization ( ECNICI )
GC -
MS /
MS and negative electrospray ionization (
NESI ) LC - MS / MS methodologies indispensable in experimental and clinical settings on oxidative and nitrative
oleic acid metabolism . -
pubmed
On the basis of the influence of the
oxygen content in solution on the photocurrent generation , the enzymatic activity of
glucose oxidase is evaluated in solution . -
pubmed
We show that the inducing effect of
NRB on the PT ( i ) is inhibited by the selective ligands of the
18kDa outer membrane ( OMM ) translocator protein (
TSPO , formerly peripheral
benzodiazepine receptor )
protoporphyrin IX ,
N , N - dihexyl-2 -( 4 - fluorophenyl ) indole-3 - acetamide and
7 - chloro-5 -( 4 - chlorophenyl )- 1,3 - dihydro-1 - methyl-2H-1,4 - benzodiazepin-2 - one ; and ( ii ) is lost in
digitonin mitoplasts , which lack an intact
OMM . -
pubmed
Our results also indicated that
SH2 domains coevolved and the number of the domains expanded alongside
protein tyrosine kinases and
tyrosine phosphatases , thereby coupling
phosphotyrosine signaling to downstream signaling networks . -
pubmed
Recently , it was demonstrated that a specialized
Ca ( 2 +)-activated anion - channel ,
bestrophin1 (
Best1 ) , found on glial cells , can release
GABA that contributes up to 50 - 75 % of the tonic GABAergic current . -
pubmed
However , it is unknown if
ethanol has any actions on
Best1 function . -
pubmed
We attempted to measure the
Best1 - mediated component of the tonic current in slices using
5 - nitro-2 -( 3 - phenylpropylamino ) benzoic acid (
NPPB ) . -
pubmed
To better isolate the
Best1 - dependent tonic current component , we blocked the Golgi cell component of the tonic current with
tetrodotoxin and found that
NPPB similarly and significantly potentiated the tonic current amplitude and decay time of miniature IPSCs . -
pubmed
Two other
Cl (-)- channel blockers were also tested :
4 '- diisothiocyanatostilbene-2,2 '- disulfonic acid disodium salt hydrate (
DIDS ) showed no effect on GABAergic transmission , while
niflumic acid (
NFA ) significantly suppressed the tonic current noise , as well as the mIPSC frequency , amplitude , and area . -
pubmed
These data suggest that acute
ethanol exposure does not modulate
Best1 channels and these findings serve to challenge recent data indicating that these channels participate in the generation of tonic GABAergic currents in cerebellar GCs . -
pubmed
Laninamivir octanoate ( LO ) (
Inavir ; Daiichi Sankyo , Japan ) is an
ester prodrug of the
neuraminidase inhibitor
laninamivir . -
pubmed
These results demonstrated that the prolonged high retention of
laninamivir in the respiratory tissues was attributed to a consecutive series of three steps : uptake of LO into the airway epithelial cells , hydrolysis of LO into
laninamivir by intracellular
esterase(s ) , and limited efflux of the generated
laninamivir due to its poor membrane permeability . -
pubmed
ZBTB1 depletion impairs formation of
phospho -
KAP-1 at UV damage sites and reduces
RAD18 recruitment . -
pubmed
We propose that
ZBTB1 is required for localizing
phospho -
KAP-1 to chromatin and enhancing
RAD18 accessibility . -
pubmed
The availability of a large quantity of the powder sample allows us to determine the crystal and magnetic structures by neutron powder diffraction (
NPD ) at 300 and 1.3 K. Measurements of the magnetization and heat capacity showed a ferrimagnetic transition at 160 K. A ferrimagnetic structure consisting of the uncompensated antiferromagnetic coupling between neighboring collinear
copper and
iron spins has been resolved from the low - temperature NPD data . -
pubmed
After
infection control measures and prolonged decontamination have been implemented with a high adherence , six patients remained sustained CA -
MRSA USA300 carriers , including one who developed
mupirocin resistance and six who experienced minor CA -
MRSA - related
SSTIs . -
pubmed
CA -
MRSA decontamination using
mupirocin and
chlorhexidine in the community setting may also be a questionable strategy , associated with failure and resistance to both agents . -
pubmed
After
nucleotide(s ) are incorporated across from the altered base(s ) , the aberrant primer termini are typically extended by
DNA polymerase zeta (
pol zeta ) . -
pubmed
Here we used brain penetrant
tyrosine kinase inhibitors ( TKIs ) , including
nilotinib and
bosutinib and showed that they reduce the level of nuclear
TDP-43 , abrogate its effects on
neuronal loss , and reverse cognitive and motor decline . -
pubmed
Nilotinib decreased soluble and insoluble
TDP-43 , while
bosutinib did not affect the insoluble level . -
pubmed
Along with elevation in serum and hippocampal
TNF - alpha and
corticosterone levels associated with significant increase in hippocampal
kynurenine /
tryptophan ratio . -
pubmed
In particular , the effects of
vasoactive intestinal peptide and
secretin on intra - acinar cell
adenosine 3 ' , 5 '- cyclic monophosphate are explored . -
pubmed
Considerable attention is paid to regulation of beta - cell function and includes studies detailing the mechanisms of regulation of
insulin by
somatostatin ,
serotonin , and melanocortins . -
pubmed
BACKGROUND :
Zanamivir is a
neuraminidase (
NA ) inhibitor used for the treatment of
influenza . -
pubmed
The combination of high selectivity of aptamer with the
peroxidase - mimicking property of DNAzyme has presented considerable opportunities for designing colorimetric aptasensor for detection of
ochratoxin A (
OTA ) . -
pubmed
The presence of
OTA triggers the opening of the hairpin structure and the beginning of
HCR , which results in the release of many DNAzyme , and generates enhanced colorimetric signals , which is correlated to the amounts of
OTA with linear range between 0.01 to 0.32 nM , and the limit of detection is 0.01 nM under optimal conditions . -
pubmed
Coordinated
GnRH1 release , not simply elevated absolute levels , effects the release of pituitary gonadotropins that drive
steroid production in the gonads . -
pubmed
We report that
GnRH1 neurons are strongly and uniformly interconnected by electrical synapses that can drive spiking in connected cells and can be reversibly blocked by
meclofenamic acid . -
pubmed
Among 46 differential metabolites found in the ultra - performance liquid chromatography quadrupole time of flight mass spectrometry ( UPLC - Q -
TOF /
MS ) and
( 1 ) H NMR - based urinary metabonomics , 20 were significantly correlated with the preferred
sucrose consumption observed in behavior experiments and were considered as biomarkers to evaluate the antidepressant effect of CSGS . -
pubmed
While the chemical constituents of Bai - Shao contributed to CSGS were
paeoniflorin ,
albiflorin ,
isomaltopaeoniflorin , and
benzoylpaeoniflorin based on the multivariate analysis of the UPLC - Q -
TOF /
MS chemical profiles from CSGS and QBS extracts . -
pubmed
NO production , cell viability ,
cytokines profile were evaluated . -
pubmed
BRP at 50 mug / ml inhibited
NO production by 78 % without affecting cell viability . -
pubmed
We have tried to use
quartz crystal microbalance ( QCM ) , which is widely used to analyze interaction among macromolecules , to detect proteolytic fragments of
TnT during aging of meat . -
pubmed
Erratum to : Prescribing pattern and resource utilization of
monoamine oxidase - B inhibitors in Parkinson treatment : comparison between
rasagiline and
selegiline . -
pubmed
N changes were noted
post supplementation for
cortisol or
DHEA , but
testosterone was found to be significantly reduced in both diary treatments compared to control ( F = 4.27 , p = .022 ) . -
pubmed
RESULTS : There was a decreasing linear effect on the intake of DM , organic matter (
OM ) , crude protein (
CP ) , neutral detergent fiber (
NDF ) , total
carbohydrates ( TCH ) , and nonfibrous
carbohydrates ( NFC ) . -
pubmed
The apparent digestibility of DM ,
OM ,
CP ,
NDF , TCH , and NFC , as well as urine
nitrogen excretion ,
nitrogen balance and ruminal parameters , were not influenced by different levels of residual frying oil in the diet . -
pubmed
The
sterol - regulatory element - binding protein (
SREBP ) pathway may contribute to the underlying pathophysiology of
AAP - induced metabolic adverse effects . -
pubmed
We explored the association between the variants of the
sterol - regulatory element - binding transcription factor-1 (
SREBF1 ) gene and the
SREBP cleavage - activation protein (
SCAP ) gene with
AAP - induced
MetS in a genetic case - control study . -
pubmed
CONCLUSIONS : The genetic polymorphisms of
SREBF1 could play a role in the mechanism for interindividual variation of
AAP - induced
MetS. -
pubmed
The main enzyme involved in the termination of
cGMP effects is
phosphodiesterase enzyme 5 (
PDE-5 ) , which is overexpressed in
ventricular hypertrophy and
heart failure . -
pubmed
Keywords used for search included
cGMP ( mechanisms and functions ) , EDs ( drugs used ) ,
nitric oxide , and
PDE-5 inhibitors ( clinical applications ) . -
pubmed
In addition , we provide evidence that heterogeneous
octopamine receptor localization , coupled with local differences in
phosphodiesterase activity , underlies the observed differences in
cAMP signaling in the axon , cell body , and boutons . -
pubmed
Seven of the variables considered explained a proportion of the education -
T2DM relationship ( body mass index ,
alcohol consumption ,
hypertension , fasting
triglycerides ,
high - density lipoprotein (
HDL )
cholesterol , physical activity and smoking status ) , where the contribution of the variables ranged from 1.0 % to 17.7 % . -
pubmed
Exogenous expression of wild - type
PNPT1 , but not mutants , rescued
ATP production in patient skin fibroblasts , suggesting the pathogenicity of the identified mutations . -
pubmed
Eluxadoline (
Viberzi ): A
Mu - Opioid Receptor Agonist for the Treatment Of
Irritable Bowel Syndrome With
Diarrhea . -
pubmed
miR-339 - 5p downregulation contributes to
Taxol resistance in
small - cell lung cancer by targeting
alpha1,2 - fucosyltransferase 1 . -
pubmed
Spectroscopic investigation of the anticancer alkaloid
piperlongumine binding to
human serum albumin from the viewpoint of drug delivery . -
pubmed
Piperlongumine (
PL ) is a very promising natural agent with a high potential for
cancer treatment . -
pubmed
Displacement studies indicated that
PL and
warfarin had separate binding regions in site I. -
pubmed
Prediction of outcome using pretreatment
18F - FDG PET /
CT and MRI radiomics in locally advanced
cervical cancer treated with chemoradiotherapy . -
pubmed
PURPOSE : The aim of this study is to determine if radiomics features from
18fluorodeoxyglucose (
FDG ) positron emission tomography / computed tomography ( PET /
CT ) and magnetic resonance imaging ( MRI ) images could contribute to prognoses in
cervical cancer . -
pubmed
We supplement atomistic calculations with thermodynamic arguments to show that codons for ' phase 1 '
amino acids ( those appearing early in evolution ) are more likely than a lower entropy
GC - rich sequence to form triplets under tension . -
pubmed
The objective of this study was to evaluate ADMI color removal from a biologically treated textile mill effluent by heterogeneous photocatalysis with UV - visible irradiation ( UV - vis ) using a novel catalyst composed of
TiO2 supported on
hydrotalcite and doped with
iron oxide (
HT /
Fe /
TiO2 ) . -
pubmed
Photocatalytic treatments were carried out with
TiO2 and
HT /
Fe /
TiO2 of
Fe :
Ti molar ratios of 0.25 , 0.5 , 0.75 and 1 , with varying catalyst doses ( 0 - 3 mg / L ) , initial pH values ( 4 - 10 ) and UV - vis times ( 0 - 6 h ) . -
pubmed
The most efficient composite was
HT /
Fe /
TiO2 1 at pH 10 , also at a dose of 2 g / L , which provided more complete ADMI color removal , from 303 to 9 ADMI color units ( 96 % ) , than unmodified
TiO2 , from 303 to 37 ADMI color units ( 88 % ) , under the same conditions . -
pubmed
For this reason , the
HT /
Fe /
TiO2 1 composite performed better at pH 10 , because the higher concentration of
hydroxide ions present at higher pH favored
hydroxyl radical formation . -
pubmed
HT /
Fe /
TiO2 1 was reused five times and still lowered secondary effluent ADMI color below local discharge limits . -
pubmed
Benefits of the
HT /
Fe /
TiO2 1 catalyst compared to
TiO2 include its lower bandgap energy ( 2.34 eV vs 3.25 eV ) , higher ADMI color removal and its magnetic nature that facilitated its recovery and would reduce treatment costs . -
pubmed
The only medication in this group approved for the treatment of
CD is
pasireotide , a
somatostatin receptor ligand . -
pubmed
Mechanism of action and available data on efficacy and safety of cell cycle inhibitor
roscovitine ,
epidermal growth factor receptor inhibitor
gefitinib ,
retinoic acid , and
silibinin , a
heat shock protein 90 inhibitor are also presented . -
pubmed
Additionally , elevated preoperative
suPAR but not
creatinine levels were a predictor of
acute kidney injury (
AKI ) after CRLM resection , correlating with a longer postoperative hospitalization . -
pubmed
We further provide a liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) approach that can separate and quantify the four normal
DNA bases ( A , T , G , and C ) , as well as the four modified
cytosine bases (
5 - methyl ,
5 - hydroxymethyl ,
5 - formyl , and
5 - carboxyl ) . -
pubmed
These results underpin the contribution of
magnesium ,
TRPM7 and
MagT1 to autophagy and osteoblastogenesis . -
pubmed
Older age ( P & lt ; 0.01 ) , a longer duration of
fever ; total and before admission ( P & lt ; 0.01 ) , more negative
nitrite test ( P & lt ; 0.01 ) , higher platelet count ( P = 0.04 ) , increased
C - reactive protein (
CRP ) ( P & lt ; 0.01 ) and erythrocyte sedimentation rate ( ESR ) ( P & lt ; 0.01 ) , were identified as initial features of infants finally diagnosed with
KD . -
pubmed
Mean + -
SD I -
PSS and quality of life scores ( 15.9 + - 6.5 vs 6.8 + - 5.6 and 3.1 + - 1.4 vs 1.5 + - 1.4 , respectively , each p & lt;0.001 ) were significantly improved after
holmium laser enucleation of the prostate compared to preoperative values . -
pubmed
FGY-1153 treatment inhibited the expression of
TGFbeta and the phosphorylation of
SMAD2 in the heart . -
pubmed
This long - term cyanobacterial
recurrence also highlights the past presence of both the
anaC and
mcyB genes , involved in
anatoxin - a and
microcystin biosynthesis , respectively , throughout the core . -
pubmed
Here , we demonstrate this polaron decoupling effect on the photochemical dynamics in singlet fission (
SF ) of amorphous
rubrene thin films embedded in optical microcavities . -
pubmed
Body weight , serum biochemical lipid parameters , and those for liver and kidney functions , serum TC ,
LDL - C ,
vLDL - C ,
HDL - C ,
TG , atherogenic index ( AI ) ,
ALT , AFT ,
ALP ,
gamma - GT ,
total protein (
TP ) ,
serum albumin and
globulin ratio ( A / G ) , serum
creatinine , blood
urea nitrogen (
BUN ) and blood
urea , and histopathology of
hematoxylin and
eosin (
H&E ) stained liver and kidney sections of all aforementioned groups were examined in the treated animals . -
pubmed
Our aim was to study whether a three - meal diet ( 3Mdiet ) with a
carbohydrate - rich breakfast may upregulate
clock gene expression and , as a result , allow dose reduction of
insulin , leading to
weight loss and better glycemic control compared with an isocaloric six - meal diet ( 6Mdiet ) . -
pubmed
Body weight , glycemic control , continuous
glucose monitoring ( CGM ) , appetite , and
clock gene expression were assessed at baseline , after 2 weeks , and after 12 weeks . -
pubmed
Upregulation of
clock genes seen in this diet intervention could contribute to the improved
glucose metabolism . -
pubmed
The
bleeding group had values of lower
albumin after 3 months of operation ( HR 0.28 , 95 % CI ( 0.11 - 0.73 ) , p = .01 ) , higher
bilirubin after 3 months of operation ( total
bilirubin : HR 1.18 , 95 % CI ( 1.04 - 1.33 ) , p = .01 ) , ( direct
bilirubin : HR 1.21 , 95 % CI ( 1.05 - 1.41 ) , p = .01 ) . -
pubmed
Propofol exhibits a
tumor - suppressive effect and regulates cell viability , migration and invasion in
bladder carcinoma by targeting the microRNA-10b /
HOXD10 signaling pathway . -
pubmed
An efficient and bio - based alginate pillared
hydrotalcite ( SA@LDHs ) was fabricated via calcination - reconstruction manner with
sodium alginate (
SA ) and
hydrotalcite ( LDHs
- C ) , and used as novel flame retardant for polypropylene ( PP ) . -
pubmed
Two common strategies to feed the
CO2 are pH steady ( PS ) and
CO2 steady (
CS ) . -
pubmed
The optimum concentration of
CO2 was estimated based on the PS cultivation data and fed to culture media using the
CS approach with no buffering agent . -
pubmed
The biomass productivity ,
CO2 bio - fixation rate , and rubisco activity under CSBPS strategy were 127 , 121 , and 65 % higher than 0.04 %
CS strategy , respectively . -
pubmed
acetyl - CoA carboxylase (
accD ) and
ribulose bisphosphate carboxylase large chain (
rbcL ) ) was evaluated using real - time PCR under different initial
iron feeds . -
pubmed
In 90 microM initial
iron concentration , the expression of
accD and
rbcL genes showed a 4.8- and 35 - fold increase , respectively , compared to that of the control genes . -
pubmed
Finally , in this paper , different mechanisms where
iron affects
acetyl - CoA carboxylase (
ACCase ) and
1,5 - ribulose bisphosphate carboxylase / oxygenase (
RuBisCo ) are illustrated . -
pubmed
Gallic acid (
GA ) is a natural
polyphenol , which exhibits a broad spectrum of biological activities , but its therapeutic application was limited due to poor bioavailability and toxicity . -
pubmed
GA was chemically conjugated with 4.0 G
PAMAM dendrimer at outer surface and characterized by different biophysical methods . -
pubmed
In conclusion , findings of this study revealed that
PAMAM - GA conjugate improves the bioavailability of
GA and specificity towards
cancer cellsto manifests apoptotic cell
death . -
pubmed
The findings of the
AMG model suggest that a 1 % increase in energy price leads to a 0.02 % decrease in
carbon emission . -
pubmed
Evaluation of the Effects of
Nobiletin on
Toll - Like Receptor 3 Signaling Pathways in
Prostate Cancer In Vitro . -
pubmed
Therefore , we aimed to evaluate the effects of
NOB on the activation of
TLR3 signaling pathways in
PCa In Vitro . -
pubmed
However , the level of
TRIF protein and
CASP8 decreased after both
NOB and Poly I : C incubation . -
pubmed
SLC39A1 is a
zinc ion transport protein which inhibits the progression of
prostate cancer . -
pubmed
Log - rank tests for univariate analysis ( gender , age , ethnicity and deprivation ) effects on serum
creatinine testing at
dabigatran etexilate treatment initiation and 12 - months
post initiation were performed . -
pubmed
The associations among
organophosphate pesticide exposure , oxidative stress , and genetic polymorphisms of
paraoxonases in children with
attention deficit / hyperactivity disorder . -
pubmed
This study will help to clarify the relationship between
organophosphate pesticides ( OPs ) and
attention deficit / hyperactivity disorder (
ADHD ) related to oxidative stress and
paraoxonases (
PON ) polymorphisms to further characterize the gene - environment interaction . -
pubmed
Oxidative stress biomarkers , such as
8 - hydroxy-2 - deoxyguanosine (
8 - OHdG ) ,
8 - nitroguanine (
8 - NO2 - Gua ) ,
8 - iso - prostaglandin F2alpha (
8 - iso - PGF2alpha ) , and
4 - hydroxy-2 - nonenoic acid - mercapturic acid (
HNE - MA ) , were analyzed by using liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) . -
pubmed
The estimated AP value was 0.66 ( 95 % CI : 0.17 - 1.15 ) , indicating that 66 % of
ADHD cases in
DMP - exposed children with the
PON1 CT / TT ( rs705381 ) genotype were due to gene - environment interactions . -
pubmed
Affinity labeling of
gamma - glutamyl transpeptidase of
tumor cell AH-130 and transport activity of
glutathione and
amino acids . -
pubmed
A complex of
immunoglobulin A (
IgA ) with
peroxidase was obtained as a result of interaction of
IgA with the
enzyme , modified with
sodium periodate . -
pubmed
The effect of two anticholinergic drugs ,
Ipratropium bromide , nonselective
muscarinic receptor antagonist and
Pirenzepine , a selective
M1 receptor blocker on
bronchospasm induced by
metacholine and
histamine was investigated . -
pubmed
It was shown that both drugs protect significantly
metacholine induced
bronchospasm , but
Ipratropium was more effective than
Pirenzepine , which suggest that blockade of the
M1 receptors only is not sufficient for a complete limitation of bronchi response to
metacholine challenge . -
pubmed
Cellular
cAMP levels peaked after two h of
FSK treatment and remained elevated for 48 h.
Chromogranin B mRNA increased with
FSK treatment , reaching a maximum of 7 - fold above control after 24 h , while the level of
SgII mRNA decreased to a level of 65 + /- 10 % of control after 48 h. The effects of FSK on
CgB mRNA appear to be mediated by
cAMP , as
8 - bromo - cAMP ( 500 microM ) resulted in a 2.8 - fold increase in
CgB mRNA , and
H-89 ( 30 microM ) , a selective inhibitor of
cAMP - dependent protein kinase , reduced the FSK - mediated response . -
pubmed
The intensity of
SgII staining was diminished by
FSK treatment , most likely a result of a decreased rate of synthesis as well as an increase in the release of
SgII . -
pubmed
Puromycin applied 15 min before and during
dexamethasone treatment blocked the induction of this mRNA , suggesting that additional
glucocorticoid induced transcription factor proteins may be required for enhanced
calmodulin gene transcription . -
pubmed
A two - fold increase in the intensity of an approximately 18 K immunoreactive
calmodulin protein band was detected by immunoblotting at 90 min after
dexamethasone administration . -
pubmed
Corticotropin releasing factor , added for 30 min at the start of
steroid treatment , prevented the increase of
calmodulin mRNA , as well as the suppression of
corticotropin releasing factor - evoked ACTH release caused by
dexamethasone . -
pubmed
Effects of
staurosporine , K 252a and other structurally related
protein kinase inhibitors on shape and locomotion of
Walker carcinosarcoma cells . -
pubmed
The structure / activity relationship of the
protein kinase inhibitors ,
staurosporine and K 252a and their analogues on motility of
Walker carcinosarcoma cells has been studied in vitro . -
pubmed
CGP 41251 , a
staurosporine derivative with a much higher specificity for
protein kinase C (
PKC ) than
staurosporine , induces a dose - dependent increase in the proportion of polarised cells , and stimulates cell locomotion . -
pubmed
Two
K252a analogues ,
KT 5720 and
KT 5822 , which act preferentially on
cyclic nucleotide - dependent protein kinases , and
CGP 42700 , an inactive
staurosporine analogue , had no effect on cell polarity and locomotion . -
pubmed
The blocking activity of the eluted MP+
HS - PG bands depended partly on the amount of GAG attached to the
protein core and presumably partly on the structure of the core itself . -
pubmed
Carboxypeptidase E (
CPE ) catalyses the removal of C - terminal basic
amino acids and is implicated in the processing of peptides and hormones in secretory vesicles . -
pubmed
Fractionation of the intracellular forms of
CPE with
Triton X-114 at various pH values indicates that the membrane - bound , but not the soluble , form is amphipathic ; this difference probably arises from
post - translational modification of the enzyme . -
pubmed
Thymocyte subsets have been well characterized on the basis of
CD4 and
CD8 antigen expression . -
pubmed
Using three - colour immunofluorescence , we show that ( i ) while most CD45RA+ cells are present amongst the
CD4 -
CD8- thymocyte subset in the normal thymus , after
cortisone treatment or irradiation , all four thymocyte subsets co - express significant amounts of
CD45RA . -
pubmed
Only
IL-1 beta-(208 - 240 ) enhanced non - rapid - eye - movement sleep and body temperature , although both
IL-1 beta-(208 - 240 ) and
IL-1 alpha-(223 - 250 ) stimulated
PGE2 production ; both of these peptides failed to stimulate T - cell production . -
pubmed
Biochemically , the most diagnostic alteration is a marked reduction in blood
copper and
ceruloplasmin levels . -
pubmed
Physiological coagulation inhibitors (
protein S ,
protein C and
antithrombin III ) in severe
preeclamptic states and in users of
oral contraceptives . -
pubmed
The clinical utility of the
carbonic anhydrase (
CA ) inhibitor
acetazolamide (
ACTZ ) is limited because of rapid development of tolerance to its effects . -
pubmed
The percent anergic infants decreased and serum
IgA increased significantly only in
Zn - supplemented group . -
pubmed
Receptor concentration was determined by measuring binding of the
muscarinic antagonist
1 - quinuclidinyl [ phenyl-4 - 3H ] benzilate (
3H - QNB ) . -
pubmed
These observations suggested that measurement of
3H - QNB binding would provide a reliable estimate of
muscarinic receptor number on cultured sympathetic neurons . -
pubmed
In contrast to the
carbachol - induced down - regulation , the reduction in binding after RFCM treatment was not prevented by
atropine , indicating that the effect was not mediated by
acetylcholine stimulation of
muscarinic receptors . -
pubmed
For this purpose , the
glycans of the
protein were liberated from the
polypeptide chain by
hydrazinolysis . -
pubmed
The chemical structures of two minor
oligosaccharide units were determined with the aid of
exoglycosidases , and by methylation analysis and Smith degradation . -
pubmed
[ Regulation of the
renin -
angiotensin system in
congestive heart failure : indications for a total body
sodium - dependent regulation of the
renin secretion ] . -
pubmed
The
thrombi were treated with
plasmin or
sodium chloride and the radioactivity released in the supernatant by the
thrombus was determined . -
pubmed
Immunoprecipitates containing
SM P180 and
SM P120 exhibited a
protein kinase activity capable of phosphorylating
tyrosine residues of both
viral gene products but not
immune immunoglobulin G molecules . -
pubmed
By independently immunoprecipitating each of the two
SM - FeSV proteins we found that most of the
tyrosine - specific phosphorylating activity was associated with the
SM P120 molecule . -
pubmed
In vivo analysis of
32P - labeled
SM P180 and
SM P120 revealed their phosphoprotein nature ; however , both molecules exhibited low levels of phosphorylation and did not contain
phosphotyrosine residues . -
pubmed
Other hormones , including somatomedins ,
epidermal growth hormone , sex
steroids , and
vitamin D metabolites , are also involved in the control of growth . -
pubmed
Sodium selenite inhibited
AHH activity to a maximum of approximately 70 % . -
pubmed
Sodium thiosulphate also inhibited
AHH activity by approximately 47 % . -
pubmed
Measurements from the triceps surae were made at
rest , and before and after prolonged exercise ( uphill walking ) at approximately 75 % VO2 max in low muscle glycogen ( low
CHO ) and high muscle glycogen ( high
CHO ) conditions . -
pubmed
One hundred unrelated patients with
type IIa hyperlipoproteinemia have been investigated for their
HLA - A and B
antigens and compared to 171 normal controls . -
pubmed
Release of
gastric inhibitory polypeptide (
GIP ) during
calcium infusion and in
hyperparathyroidism . -
pubmed
This caused
GIP to respond significantly more to
glucose ingestion . -
pubmed
The potentiated response of
insulin to
glucose may be caused , in part , by
GIP . -
pubmed
Compounds such as
insulin were added to the cells upon release from the block to sensitize the cells to the
carcinogen that was added during S. Growth of the transformed cells as distinct from nontransformed cells was promoted by growth in medium supplemented with 8X nonessential
amino acids . -
pubmed
One possible target for chemotherapy is the biosynthesis of the
glycosyl phosphatidyl - inositol (
GPI ) of this parasite 's variant surface glycoprotein (
VSG ) . -
pubmed
Their incorporation into
VSG correlated roughly with toxicity , although they also were incorporated into
phospholipids and other proteins . -
pubmed
Myristate analogs are useful for studying the mechanism of
GPI myristolyation , and they are candidates for antitrypanosomal chemotherapy . -
pubmed
Changes in
sex hormone binding globulin , total and free
testosterone ,
dihydrotestosterone and
delta 4 - androstenedione were found to be independent of the
epileptic syndrome type . -
pubmed
Among antiepileptic drugs ,
PHT is associated with higher
sex hormone binding globulin and
estradiol and lower free
testosterone and
dihydrotestosterone levels . -
pubmed
PB and
CBZ , but not
PHT , blunt the
LH response to exogenous
LHRH in
PE . -
pubmed
The present study is a more extensive examination of sex differences in immunoreactive
inhibin and
steroidal hormones in term umbilical cord blood . -
pubmed
The concentration of
dehydroepiandrosterone sulphate (
DHEAS ) and the
sex hormone - binding globulin (
SHBG ) capacity were similar in males and females . -
pubmed
Inhibin did not correlate with
DHEAS either in their arterial serum concentrations or in the arteriovenous concentration differences . -
pubmed
Plasma
glucose was analysed by a
glucose oxidase /
oxygen electrode methodology . -
pubmed
Sequence comparisons between parental and
mutant HN proteins revealed multiple non - adjacent
amino acid substitutions in the majority of MAR mutants . -
pubmed
L1 is an axonal cell adhesion
molecule found primarily on projection axons of both the CNS and PNS . -
pubmed
CKII was able to phosphorylate a peptide encompassing
amino acids ( aa ) 1,173 - 1,185 , as well as a related peptide representing an alternatively spliced nonneuronal
L1 isoform that lacks aa 1,177 - 1,180 . -
pubmed
Serine to
alanine substitutions in these peptides indicate that the
CKII phosphorylation site is at
Ser1,181 . -
pubmed
Instead , in this study , an increase in anastomotic
prostacyclin production over unsutured controls ( control 1093 + /- 222 pg / ml of
6 - keto prostaglandin F (
PGF ) 1 - alpha , n=21 ; anastomosis 2494 + /- 414 , n=21 , mean + /- 1 SEM , p=0.005 ) is demonstrated . -
pubmed
MAIN OUTCOME MEASURES :
Glucose ,
cortisol ,
androgens ,
estrogens , thyroid hormones , and
insulin growth - like factor (
IGF - I ) were measured in basal samples . -
pubmed
The
GH responses to
GH - RH alone ,
pyridostigmine alone , or the combination were lower in the older than in the younger group and were correlated negatively with age . -
pubmed
In contrast , either
arginine alone or
GH - RH plus
arginine produced similar
GH responses in the two groups . -
pubmed
Adrenergic and
purinergic receptors - mediated
calcium responses in brown adipocytes . -
pubmed
L - Arginine alone failed to affect basal or stimulated (
CCK or yolk ) emptying of the GB , but when combined with
L - NMMA , it reversed the enhancement of GB contraction caused by
L - NMMA . -
pubmed
The basal plasma
CCK level was 1.2 + /- 0.3 pmol / l and rose to 7.9 + /- 2.1 pmol / l with
CCK8 infusion and to 4.7 + /- 1.8 pmol / l after yolk meal . -
pubmed
No significant change in plasma
CCK levels was observed in tests with
L - NMMA and/or
L - arginine . -
pubmed
We conclude that under physiological conditions , the GB contractile activity is regulated predominantly by
CCK , but endogenous
nitric oxide has a tonic relaxing influence on this activity . -
pubmed
No differences in either hyaluronate or
collagen were found in either
strain and 14 days of development and
dexamethasone treatment decreased these compounds in both
strains . -
pubmed
This machinery consists of 3 ' to 5 ' mRNA decay activities that are
Mg2 + -dependent , require neither exogenous
ATP /
GTP nor an
ATP - regenerating system , and act independently of a 7mG(5')ppp(5')G cap structure to deadenylate an exogenous mRNA substrate in a
c - myc 3'-UTR - dependent fashion . -
pubmed
Replacing
Val with
Pro at
P2 in this sequence greatly improved the rate of cleavage by
cruzipain . -
pubmed
Tyr and
Val residues are preferred at
P3 by all
cysteine proteinases whatever their origin , whereas only
cruzipain and
cathepsin L cleaved substrate with a
His at that position . -
pubmed
The combination of a
Pro at
P2 and
His at
P3 abolished cleavage by
cathepsin L , so that only
cruzipain was able to cleave the HPGGP peptide at the GG bond . -
pubmed
A substrate with intramolecularly quenched fluorescence was raised on this sequence (
Abz - HPGGPQ - EDDnp ) which was also specifically cleaved by
cruzipain ( kcat / Km of 157 000 m-1 . -
pubmed
The pH activity profile of
cruzipain on
Abz - HPGGPQ - EDDnp showed a narrow peak with a maximum at pH 5.5 and no cleavage above pH 6.8 , although trypanosomal
cysteine proteinases remain active at basic pH. -
pubmed
The importance of the
His residue at
P3 for cleavage by
cruzipain was confirmed by substituting
Lys for
His at that position . -
pubmed
It was concluded that the presence of an
imidazolium group at
P3 was essential to endow the HPGGPQ sequence with the properties of a
cruzipain substrate . -
pubmed
Prostate - specific antigen (
PSA ) promoter - driven
androgen - inducible expression of
sodium iodide symporter in
prostate cancer cell lines . -
pubmed
Causing
prostate cancer cells to express functionally active
sodium iodide symporter (
NIS ) would enable those cells to concentrate
iodide from plasma and might offer the ability to treat
prostate cancer with
radioiodine . -
pubmed
The
PSA promoter is responsible for
androgen - dependent expression of
PSA in benign and malignant prostate cells and was therefore used to mediate
androgen - dependent prostate - specific expression of
NIS . -
pubmed
Prostate cells transfected with
NIS /
PSA - pEGFP-1 showed
perchlorate - sensitive ,
androgen - dependent
iodide uptake in a range comparable to that observed in control cell lines transfected with
hNIS cDNA . -
pubmed
Perchlorate - sensitive
iodide uptake was not observed in cells transfected with
NIS /
PSA - pEGFP-1 and treated without
androgen or in cells transfected with the control vectors . -
pubmed
In conclusion , tissue - specific
androgen - dependent
iodide uptake activity has been induced in
prostate cancer cells by
PSA promoter - directed
NIS expression . -
pubmed
Androgen and
estrogen signaling at the cell membrane via
G - proteins and
cyclic adenosine monophosphate . -
pubmed
The system consists of three components , an agonist
steroid ,
sex hormone - binding globulin (
SHBG ) , and a membrane receptor ( R(
SHBG ) ) for
SHBG . -
pubmed
SHBG is a well - characterized plasma protein that has two binding sites , one binds certain
estrogens and
androgens , and the other binds to R(
SHBG ) . -
pubmed
The characteristics of this novel signal transduction system , from the interaction of
SHBG with R(
SHBG ) , to the intermediacy of
G - proteins , to
cAMP generation , to downstream effects of the second messenger will be reviewed . -
pubmed
When cells were incubated in 25 mM
glucose to mimic the
diabetic state ,
IL-1beta induction was inhibited in all cases , but to a significantly lesser extent with the 14 - repeat allele . -
pubmed
[ Intrinsic
nucleoside diphosphate kinase - type activity is a novel function of the molecular chaperone and
protease ] . -
pubmed
We examined the function of the
V - ATPase in cytosolic
Ca ( 2 + ) homeostasis by comparing responses to a brief
Ca ( 2 + ) challenge of a
V - ATPase mutant (
vma2Delta ) and wild - type cells treated with the
V - ATPase inhibitor
concanamycin A. -
pubmed
The kinetics of the
Ca ( 2 + ) response were determined using transgenic
aequorin as an in vivo cytosolic
Ca ( 2 + ) reporter system . -
pubmed
In wild - type cells , the
V - ATPase - driven
Vcx1p was chiefly responsible for restoring cytosolic
Ca ( 2 + ) concentrations after a brief
pulse . -
pubmed
In cells lacking
V - ATPase activity , brief exposure to elevated
Ca ( 2 + ) compromised viability , even when there was little change in the final cytosolic
Ca ( 2 + ) concentration . -
pubmed
vma2Delta cells were more efficient at restoring cytosolic [
Ca ( 2 + ) ] after a
pulse than
concanamycin - treated wild - type cells , suggesting long term loss of
V - ATPase triggers compensatory mechanisms . -
pubmed
The objective of this study was to determine the impact of endoluminal LPRLL on vascular levels of
inducible nitric oxide synthase (
iNOS ) and
cyclic guanosine monophosphate (
cGMP ) to help define the mechanism of this effect . -
pubmed
For five members of the family of the small
leucine - rich proteoglycans (
SLRPs ) , the expression pattern during fetal development was analyzed . -
pubmed
To investigate the biochemical mechanism of the effect of chronic
insulin delivery by either route on hepatic
glucose metabolism , we measured the hepatic activity of
glucose 6 - phosphatase (
G6Pase ) and
glucokinase (
GK ) . -
pubmed
Glucose levels , higher in D , were normalized by
insulin treatment regardless of route . -
pubmed
STZ diabetes resulted in a 10 - fold decrease in
GK ( P & lt ; .001 ) , and a 2 - fold increase in
G6Pase activity ( P & lt ; .01 ) . -
pubmed
Our current studies indicate that chronic intraperitoneal
insulin treatment is superior to subcutaneous treatment with regard to hepatic
glucose metabolism . -
pubmed
Oxidant / antioxidant parameters , namely
superoxide dismutase (
SOD ) ,
glutathione peroxidase (
GSH - Px ) ,
catalase (
CAT ) ,
malondialdehyde (
MDA ) , nonenzymatic
superoxide scavenger activity ( NSSA ) , susceptibility to oxidation ( SO ) and antioxidant potential ( AOP ) values , were assayed in the samples . -
pubmed
The major lipoprotein fractions (
VLDL ,
LDL ,
HDL ) were separated by preparative ultracentrifugation , and
TG and
cholesterol concentrations were determined in plasma and lipoprotein fractions . -
pubmed
In the initial 3 - mon period , total
cholesterol remained steady , whereas
LDL - cholesterol and total
apolipoprotein B showed a significant increase . -
pubmed
No significant changes were found in total and transported
TG and
cholesterol between the 3 - mon and the 6 - yr values . -
pubmed
Post - transplant immunosuppression consisted of
tacrolimus ,
mycophenolate mofetil , and
prednisolone . -
pubmed
BMD of the lumbar spine and femoral neck decreased significantly in the
steroid+ group ( -1.4+/-3.2 % and -2.3+/-2.9 % , respectively , P<0.05 ) while no changes were observed in the
steroid- group . -
pubmed
The change in
BMD of the lumbar spine was significantly different between the
steroid+ and the
steroid- group , whereas the change in
BMD of the femoral neck was not significantly different . -
pubmed
The central section was stained for
tartrate resistant acid phosphatase (
TRAP ) in order to identify osteoclasts , one adjacent section was immunostained with an affinity purified antibody to a 15
amino acid sequence unique to
rat PTH / PTHrP receptor , and the other adjacent section in the set served as an immunostaining control . -
pubmed
In contrast to
AtlE , which is known to influence primary attachment , delta - toxin appeared to exert its effect on attachment to
polystyrene during later stages of biofilm formation . -
pubmed
Very low doses of
ethanol induce behavioral changes involving
dopamine D2 receptors . -
pubmed
These effects were inhibited by peripheral administration of the
dopamine D2 receptor antagonist ,
sulpiride ( 1 mg / kg ) . -
pubmed
The pretreatment with the
dopamine D2 receptor agonist ,
( + /-)- 2 -( N - phenethyl - N - propylamino )- 5 - hydroxytetralin (
PPHT , 0.1 mg / kg ) , enhanced the effects of
ethanol very low doses in delaying the initiation and reducing the duration of
narcosis induced by an acute i.p . -
pubmed
Thus , repeated administration of
ethanol very low doses may have affected the sensitivity of presynaptic
dopamine D2 receptors . -
pubmed
The 68 and 72 kDa molecular forms of inner ear
CTL2 are distinguished by
sialic acid modification of the
carbohydrate . -
pubmed
The
KHRI-3 antibody binds to an N - linked
carbohydrate on
CTL2 and presumably damages the organ of Corti by blocking the transporter function of this molecule . -
pubmed
The primary end point was
HbA ( 1c ) after 12 weeks ; secondary end points were fasting serum
glucose , fasting
C - peptide , fasting
glucagon , fasting
insulin , beta - cell function ,
body weight , adverse events , and
hypoglycemic episodes . -
pubmed
HbA(1c ) decreased in all but the lowest
liraglutide dosage group . -
pubmed
In the 0.75 - mg
liraglutide group ,
HbA(1c ) decreased by 0.75 percentage points ( P & lt ; 0.0001 ) and fasting
glucose decreased by 1.8 mmol / l ( P = 0.0003 ) compared with placebo . -
pubmed
The
proinsulin - to -
insulin ratio decreased in the 0.75 - mg
liraglutide group ( -0.18 ; P = 0.0244 ) compared with placebo . -
pubmed
Reduction of
dioxygen catalyzed by
pyrene - wired
heme domain
cytochrome P450 BM3 electrodes . -
pubmed
We have electronically wired the
cytochrome P450 BM3 heme domain to a
graphite electrode with the use of a
pyrene - terminated tether . -
pubmed
AFM images clearly reveal that
pyrene - wired
enzyme molecules are adsorbed to the electrode surface . -
pubmed
Routine database analyses suggested that two enzymes essential for
fatty acid synthesis were missing , one of these is the
holo - acyl - carrier - protein synthase . -
pubmed
Besides lecithin , surfactant contains a variety of minor lipids , such as plasmalogens ,
polyunsaturated fatty acid - containing phospholipids (
PUFA -
PL ) , and
cholesterol . -
pubmed
Lipid composition exhibited strong differences : Survanta had the highest proportion of disaturated PL and total neutral lipids and the lowest proportion of
PUFA -
PL . -
pubmed
In
Survanta samples , viscosity increased & gt;8 x 10(-6 ) kg / s at surface tension of 30 mN / m. Curosurf showed only slightly increased surface viscosity below surface tensions of 25 mN / m , and viscosity did not reach 5 x 10(-6 ) kg / s. By adding defined
PL to Survanta , we obtained a Curosurf - like lipid mixture ( without
plasmalogens ) that exhibited biophysical properties like Curosurf . -
pubmed
For DNA microarrays , oligonucleotide probes for these targeted genes were deposited onto
epoxysilane - derivatized , 12 - well , Teflon - masked slides by using a MicroGrid
II arrayer . -
pubmed
Direct
titanium ( 14 ) and stainless steel ( 14 )
posts were not used extensively . -
pubmed
CONCLUSIONS : The majority of the practitioners surveyed in this study used : amalgam for core build - ups ; indirect
posts ; addition - cured
silicone for impressions ; and glass - ionomer cements for luting procedures . -
pubmed
Production of extracellular
phospholipase and
protease was tested by the
agar plate methods . -
pubmed
Cerebral emboli may be composed of atherosclerotic debris ,
calcium , air , fat , platelet thrombi , or
CPB tubing . -
pubmed
The
GGDEF and EAL domain proteins involved in
c - di - GMP synthesis and degradation , respectively , are ( almost ) ubiquitous in bacterial genomes . -
pubmed
While the role of
GGDEF and EAL domain proteins in bacterial physiology and behaviour has gained appreciation , and significant progress has been achieved in understanding the enzymology of
c - di - GMP turnover , many questions regarding
c - di - GMP - dependent signalling remain unanswered . -
pubmed
The rational for this study was to determine if there is an altered risk for
liver damage by
APAP in the absence of
Mrp2 . -
pubmed
Mrp2 expression not only is important in biliary excretion , but also influences the toxic potential of reactive intermediates by controlling intrahepatic
GSH and possibly drug metabolism . -
pubmed
The biological activity of
DnaK , the bacterial representative of the
Hsp70 protein family , is regulated by the allosteric interaction between its
nucleotide and peptide substrate binding domains . -
pubmed
Despite the importance of the
nucleotide - induced cycling of
DnaK between substrate - accepting and releasing states , the heterotropic allosteric mechanism remains as yet undefined . -
pubmed
To further characterize this mechanism , the
nucleotide - induced absorbance changes in the vibrational spectrum of wild - type
DnaK was characterized . -
pubmed
To assign the conformation sensitive absorption bands , two deletion mutants ( one lacking the C - terminal alpha - helical subdomain and another comprising only the N - terminal
ATPase domain ) , and a single - point
DnaK mutant ( T199A ) with strongly reduced
ATPase activity , were investigated by time - resolved infrared difference spectroscopy combined with the use of caged -
nucleotides . -
pubmed
The results indicate that ( 1 )
ATP , but not
ADP , binding promotes a conformational change in both subdomains of the peptide binding domain that can be individually resolved ; ( 2 ) these conformational changes are kinetically coupled , most likely to ensure a decrease in the affinity of
DnaK for peptide substrates and a concomitant displacement of the lid away from the peptide binding site that would promote efficient diffusion of the released peptide to the medium ; and ( 3 ) the alpha - helical subdomain contributes to stabilize the interdomain interface against the thermal challenge and allows bidirectional transmission of the allosteric signal between the
ATPase and substrate binding domains at stress temperatures ( 42 degrees C ) . -
pubmed
Diffusion of water in
montmorillonite clays at low hydration has been studied on the microscopic scale by two quasi - elastic neutron scattering techniques , neutron spin - echo ( NSE ) and time - of - flight (
TOF ) , and by classical microscopic simulation . -
pubmed
In the case of the
Na - bihydrated system , the
TOF results are in closer agreement with the other two techniques ( the techniques differ by a factor of 2 - 3 at most ) , giving diffusion coefficients of ( 5 - 10 ) x 10(-10 ) m(2 ) s(-1 ) . -
pubmed
LC -
MS analysis of
carbonyl compounds and their occurrence in diesel emissions . -
pubmed
The aim of this work was to study the effects of the M ,
SD and its interaction on
5 - HT and
5 - hydroxy - indole - acetic acid (
5 - HIAA ) contents in the dorsal raphe ( DR ) and the suprachiasmatic nuclei (
SCN ) , two sleep - wake cycle regulators . -
pubmed
DR and
SCN were dissected and processed to determine the
5 - HT and
5 - HIAA contents by means of HPLC . -
pubmed
We conclude that the sleep - wake cycle disruptions produced by chronic M and
SD are mediated in part by a synergistic effect on
5 - HT in the DR -
SCN pathway , perhaps due to a delay in the development of such brain structures . -
pubmed
An original and novel assay system with
urease as a catalyst and
CdSe /
ZnS quantum dots ( QDs ) as an indicator has been developed for quantitative analysis of
urea . -
pubmed
The detection is based on the enhancement of QD photoluminescence (
PL ) intensity , which is correlated to the enzymatic degradation of
urea . -
pubmed
By controlling the buffer concentration and pH ,
PL enhancement due to the degradation of
urea is linear in the
urea concentration ranging from 0.01 to 100mM. -
pubmed
Here , we conducted
( 19 ) F NMR ,
CD , T(m ) , and modeling experiments at various primer positions with respect to a template modified by a
fluorine tagged AF - adduct (
FAF ) . -
pubmed
Molecular modeling indicated that the
aminofluorene moiety in the minor groove of the W - conformer would impose a steric clash with the tight - packing
amino acid residues on the DNA binding area of the Bacillus fragment ( BF ) , a replicative
DNA polymerase . -
pubmed
BACKGROUND & amp ; OBJECTIVES : Endo - derived
nitric oxide (
NO ) is synthesized from
L - arginine by
endothelium nitric oxide synthase (
eNOS ) encoded by the
NOS3 gene on chromosome7 . -
pubmed
Since reduced
NO synthesis has been implicated in the development of
coronary atherosclerosis ; polymorphisms of
NOS gene might be associated with increased susceptibility to this disorder and
coronary artery disease (
CAD ) . -
pubmed
The interaction and cellular localization of
HSP27 and
ERbeta are modulated by
17beta - estradiol and
HSP27 phosphorylation . -
pubmed
Recently , we identified
heat shock protein 27 (
HSP27 ) as an
estrogen receptor - beta (
ERbeta ) associated protein that acts as a co - repressor of
estrogen signaling and serves as a biomarker of
atherosclerosis . -
pubmed
In vitro we determined that phosphorylated
HSP27 is retained in the cytoplasm after treatment with
17beta - estradiol and to a lesser extent with heat
shock . -
pubmed
HSP27 and
ERbeta associate in both the cytoplasm and nucleus , however , co - localization studies reveal that in the presence of
17beta - estradiol , a significant portion of this interaction occurs outside of the nucleus . -
pubmed
These data highlight an extranuclear interaction between
ERbeta and
HSP27 that may be of potential importance in modulating
estrogen signaling . -
pubmed
Cannabinoid CB1 receptors of the dorsal hippocampus are important for induction of conditioned place preference (
CPP ) but do not change
morphine CPP . -
pubmed
The
cannabinoid CB1 receptor agonist (
WIN55,212 - 2 ) and antagonist (
AM251 ) were injected bilaterally into the dorsal hippocampus . -
pubmed
The conditioning treatments with injections of
morphine ( 6 and 9 mg / kg ) induced a
CPP for the drug - associated place . -
pubmed
HIF-1alpha promotes survival of prostate cells at a high
zinc environment . -
pubmed
METHODS : Immunohistochemistry , histofluorescent
zinc staining , Western blot , in vitro binding assay , immunoprecipitation ,
caspase activity assay , and proteasome activity assay . -
pubmed
Moreover , in cultured prostate cells ,
HIF-1alpha expression was
zinc - dependently induced even under normoxic conditions . -
pubmed
Mechanistically ,
zinc ions inhibited
HIF-1 - prolyl hydroxylase (
PHD ) activity , and therefore blocked
von Hippel - Lindau tumor suppressor protein (
pVHL ) binding to
HIF-1alpha in vivo and in vitro . -
pubmed
The
HIF-1alpha stabilization was mediated by oxidative stress induced by
zinc ion . -
pubmed
CONCLUSION : These results suggest that
HIF-1alpha functions as an intrinsic defense molecule that enables prostate cells to survive in a
zinc - rich environment . -
pubmed
Drugs targeting
serotonin receptors like
serotonin reuptake inhibitors ( SSRIs ) are useful in the present therapy of
anxiety and
depression . -
pubmed
The spectroscopic behavior of mono- and dinuclear
Ru ( II ) complexes ( P , T ,
PP and TT , Figure 1 ) that contain the extended planar ligand
tetrapyrido [ 3,2 - a : 2 ' , 3 '- c : 3 ' ' , 2 ' ' - h : 2 ' ' ' , 3 ' ' ' - j ] acridine (
TPAC ) and either
1,10 - phenanthroline (
phen ) or
1,4,5,8 - tetraazaphenanthrene ( tap ) as ancillary ligands is examined in water and as a function of the pH. -
pubmed
In contrast , with
phen ancillary ligands ( P and
PP ) , the protonated excited state does not luminesce . -
pubmed
Thioglycoside hydrolysis catalyzed by
beta - glucosidase . -
pubmed
Not surprisingly , in spite of the requirement for the presence of this protonated group in catalytically active
beta - glucosidase ,
thioglucoside hydrolysis does not involve general acid catalysis . -
pubmed
There is no solvent kinetic isotope effect on the
enzyme - catalyzed hydrolysis of
pNPSG . -
pubmed
DON had no effect on
collagen secretion in RPTEC cells . -
pubmed
We also studied the metabolism and the cellular uptake of
DON using LC -
MS /
MS . -
pubmed
Apart from known
steroid effects ,
calcitriol in particular promoted regulatory T cell profiles as indicated by a marked increase of
IL-10 ,
TGFbeta ,
FoxP3 , and
CTLA4 . -
pubmed
Glucose levels were & gt;11.1 mmol / l throughout 72 h of continuous
glucose monitoring , with low
insulin secretion during intravenous
glucose tolerance testing . -
pubmed
Cotransfection of
Kir6.2 and mutant
SUR1 demonstrate that the mutated protein is expressed efficiently at the cell surface but fails to respond to
MgADP , resulting in minimal channel activity . -
pubmed
PURPOSE : The aim of the study was to identify variables associated with successful long - term maintenance of
sinus rhythm (
SR ) after a single ablation of long - lasting persistent
atrial fibrillation (
AF ) . -
pubmed
Inosine monophosphate dehydrogenase type II (
IMPDH2 ) is the target for immunosuppression by
mycophenolic acid and has been linked to resistance of
tumor cells to chemotherapy . -
pubmed
The influence of
sterols on the conformation of recombinant mitochondrial
porin in detergent . -
pubmed
When isolated from mitochondria
porins copurify with
sterols , and some isolated forms of the protein require
sterol for insertion into artificial membranes . -
pubmed
Nonetheless , the contributions of
sterols to the folded state of mitochondrial
porin are not understood . -
pubmed
Sterols do not significantly alter the secondary structure of
porin in
lauryl dimethylamine oxide , nor in a mixture of
sodium dodecylsulfate and
dodecylmaltopyranoside . -
pubmed
Serum
glucose and
insulin were examined in the morning of the 3rd day before milk . -
pubmed
Gestational age ,
birth weight ,
birth weight percentage , serum
glucose ,
insulin and HOMA - IR were compared among different genotype groups . -
pubmed
RESULTS : No significant difference was found between the serum
glucose level of SGA group ( 4.03 + /- 1.05 mmol / L ) and
AGA group ( 4.05 + /- 1.14 mmol / L ) , P = 0.008 . -
pubmed
The
dermatitis was accompanied by infiltration of neutrophils and macrophages and by elevated levels of pro - inflammatory cytokines ,
chemokines and
prostaglandin E2 . -
pubmed
A series of
3 - acylaminocaprolactams are inhibitors of
chemokine - induced chemotaxis . -
pubmed
Branching of the side chain
alpha - carbon provides highly potent inhibitors of a range of CC and
CXC chemokines . -
pubmed
The majority of research for the
calcitonin gene - related peptide (
CGRP ) in the stomach in the
hypertension has been devoted to the submucosal blood flow , and no attention has been paid to its quantitative distribution in the gastric neuroendocrine cells . -
pubmed
Moreover , since angiogenesis is essential for follicle development , the effect of the
flavonoid on
Vascular Endothelial Growth Factor output by granulosa cells was also taken into account . -
pubmed
Additionally , the
flavonoid interferes with the angiogenic process by inhibiting
VEGF production as well as by altering redox status . -
pubmed
Chapter 4 : Regulation of
Clusterin activity by
calcium . -
pubmed
In this chapter , the attention is put on
Ca ( 2 + ) effect on
Clusterin (
CLU ) activity . -
pubmed
We showed that two
CLU forms ( secreted and nuclear ) are differently regulated by
Ca ( 2 + ) and that
Ca ( 2 + ) fluxes affect
CLU gene expression . -
pubmed
Based on available data we suggest , that different
CLU forms play opposite roles , depending on intracellular
Ca ( 2 + ) concentration , time - course of
Ca ( 2 + ) current , intracellular
Ca ( 2 + ) compartmentalization , and final
Ca ( 2 + ) targets . -
pubmed
Discussion will be motivated on how
CLU acts on cell in response to
Ca ( 2 + ) waves . -
pubmed
The impact of
Ca ( 2 + ) on
CLU gene activity and transcription , posttranscriptional modifications , translation of
CLU mRNA , and posttranslational changes as well as biological effects of
CLU will be discussed . -
pubmed
We will also examine how
Ca ( 2 + ) signal and
Ca ( 2 +)-dependent proteins are attributable to changes in
CLU characteristics . -
pubmed
Some elucidation of
CLU gene activity ,
CLU protein formation , maturation , secretion , and intracellular translocations in response to
Ca ( 2 + ) is presented . -
pubmed
We assume that commonly upregulated mRNA for
nCLU versus
sCLU and vice versa are dependent on
Ca ( 2 + ) accessibility and its intracellular distribution . -
pubmed
It looks as if at low intracellular
Ca ( 2 + ) the delay in cell cycle allows more time for DNA repair ; otherwise , cells undergo
nCLU - dependent apoptosis . -
pubmed
In conclusion , a narrow range of intracellular
Ca ( 2 + ) concentrations is responsible for the decision whether
nCLU is mobilized ( apoptosis ) or
sCLU is appointed to improve survival . -
pubmed
Nonetheless we feel that much work is left ahead before remaining uncertainties related to
Ca ( 2 + ) signal and the respective roles of
CLU proteins are unraveled . -
pubmed
Negative charge of the
glutamic acid 417 residue is crucial for
isomerohydrolase activity of
RPE65 . -
pubmed
RPE65 is the
isomerohydrolase essential for regeneration of
11 - cis retinal , the chromophore of visual pigments . -
pubmed
Apc ( MIN ) modulation of
vitamin D secosteroid growth control . -
pubmed
A central paradox of
vitamin D biology is that
1alpha,25 -( OH )( 2 ) D ( 3 ) exposure inversely relates to
colorectal cancer (
CRC ) risk despite a capacity for activation of both pro- and anti - oncogenic mediators including
osteopontin (
OPN)/
CD44 and
E - cadherin , respectively . -
pubmed
Here we investigate effects of the
Apc ( Min /+ ) genotype on
1alpha,25 -( OH )( 2 ) D ( 3 ) regulation of
OPN /
CD44 /
E - cadherin signalling and intestinal tumourigenesis , in vivo . -
pubmed
Treatment by
1alpha,25 -( OH )( 2 ) D ( 3 ) or rationally designed analogues ( QW or BTW ) enhanced
OPN but inhibited expression of
CD44 , the
OPN receptor implicated in cell growth . -
pubmed
All
secosteroids suppressed
Apc(Min/+)-driven tumourigenesis although QW and BTW had lower
calcium - related toxicity . -
pubmed
Taken together , these data indicate that the
Apc(
Min/+ ) genotype modulates
vitamin D secosteroid actions to promote functional predominance of
E - cadherin tumour suppressor activity within antagonistic molecular networks . -
pubmed
APC heterozygosity may promote favourable tissue- or
tumour - specific conditions for growth control by
vitamin D secosteroid treatment . -
pubmed
The absolute quantification method , on the other hand , provides us with an accurate computational tool to calculate absolute values of
oxy- ,
deoxy- , total
hemoglobin concentrations , and light scattering coefficients . -
pubmed
Laboratory test revealed a
hemoglobin of 7.7 g / dl and a bone marrow negative for
iron . -
pubmed
Up - regulation of the
G3PDH ' housekeeping ' gene by
estrogen . -
pubmed
In our studies implementing
estrogen treatments on different cell lines , we initially used
glyceraldehyde-3 - phosphate dehydrogenase (
G3PDH ) as an internal standard . -
pubmed
However , the results of PCR amplification demonstrated that
17beta - estradiol enhanced the expression of the
G3PDH gene , rendering it impossible to use
G3PDH as an unbiased comparative control . -
pubmed
DNA damage , stalled replication forks , errors in mRNA splicing , and availability of nutrients activate specific
phosphatidylinositiol-3 kinase - like kinases (
PIKKs ) that in turn phosphorylate downstream targets such as
p53 on
serine 15 . -
pubmed
Here , we demonstrate that siRNA oligonucleotides targeting the mRNA surveillance proteins
SMG1 ,
Upf1 ,
Upf2 , or the
PIKK protein
ATM attenuated
p53 (
ser15 ) phosphorylation in cells damaged by high
oxygen (
hyperoxia ) , a model of persistent oxidative stress that damages
nucleotides . -
pubmed
In contrast , loss of
SMG1 or
ATM , but not
Upf1 or
Upf2 reduced
p53 (
ser15 ) phosphorylation in response to DNA double strand breaks produced by expression of the
endonuclease I - PpoI. -
pubmed
30 + - 2 days postinfection , jejunal
EBF ( electrophysiological parameters ,
fluorescein isothiocyanate - dextran fluxes and responses to secretagogues and MMC degranulators ) was evaluated ( Ussing chamber ) . -
pubmed
spiralis - infected animals , secretory responses to
serotonin ( 5 -
HT ) and
immunoglobulin E (
IgE)-dependent activation of MMCs were reduced . -
pubmed
In contrast , responses to
substance P ( SP ) and
capsaicin were similar in infected and noninfected animals . -
pubmed
The altered permeability and the
TTX - independent hyporesponsiveness to
5 - HT and
IgE indicate epithelial alterations . -
pubmed
The mean of the indirect
bilirubin measurements for 0- , 1- , and 3 - hour samples were 174 ( SD 68.65 ) , 186.97 ( SD 60.47 ) , and 184.56 (
SD 66.93 ) , respectively . -
pubmed
Neuroimaging studies have found moderating effects of
dopamine genes during both the anticipation and delivery of rewards , particularly the
catechol - O - methyltransferase (
COMT ) genotype . -
pubmed
In anticipation of uncertain outcomes , we observed an increased
SPN among
Met homozygotes . -
pubmed
Under low levels of reactive
oxygen species ( ROS ) , " normal " amounts of
p53 upregulates expression of antioxidant genes , protecting macromolecules from ROS - induced damage . -
pubmed
CONCLUSION : The mutation acted in a E - subunit dependent manner , suggesting I(Ks ) function altered by the presence of different
KCNE subunits in
sinus node , atria and ventricles as the molecular basis of
sinus bradycardia ,
fAF and
LQTS in mutation carriers . -
pubmed
Then observed the general reaction of each group;The content of
cholesterol in the blood and brain tissue was detected ; The neurofibrillary tangles ,
beta amyloidal protein deposits and loss of midbrain neurons of hippocampus and cortex tissue were observed . -
pubmed
In time fluorescence quantitative method was used to detect the hippocampus and cortex tissue metabolism of
cholesterol key genes
CYP46a1 and
APOE expression . -
pubmed
Actually the content of
cholesterol of group
II , III increased obviously , especially group III , while decreases in group
IV after the treatment . -
pubmed
SIRT3 is a member of the
Sir2 family of
NAD(+)-dependent protein deacetylases that promotes longevity in many organisms . -
pubmed
A vital feature of
CaMKII in plasticity is its ability to switch to a
calcium (
Ca ( 2 + ) ) independent constitutively active state after autophosphorylation at
threonine 287 ( T287 ) . -
pubmed
At the cellular level
CaMKII overexpression in the alpha'/beta ' neurons increased activity dependent
Ca ( 2 + ) responses while reduction of
CaMKII decreased it . -
pubmed
Likewise reducing
CASK or directly expressing a phosphomimetic
CaMKII T287D transgene in the alpha'/beta ' similarly decreased
Ca ( 2 + ) signaling . -
pubmed
Our results are consistent with
CASK regulating
CaMKII autophosphorylation in a pathway required for memory formation that involves activity dependent changes in
Ca ( 2 + ) signaling in the alpha'/beta ' neurons . -
pubmed
In a cell - free system , however ,
ethyl p - methoxycinnamate did not directly inhibit
tyrosinase , the rate - limiting enzyme of melanogenesis . -
pubmed
These results indicate that the pigment - inhibitory effect of
ethyl p - methoxycinnamate results from downregulation of
tyrosinase . -
pubmed
Atypical zeta ( zeta ) isoform of
protein kinase C serves as a factor that integrates with peripheral
MOR pathway and
insulin signals for
glucose utilization . -
pubmed
The developments call new insights into the chemical compounds and/or herbal products that might enhance opioid peptide secretion and/or stimulate
MOR in peripheral
insulin - sensitive tissues to serve as potential agents or adjuvants for helping the
glucose metabolism . -
pubmed
Role of rostral ventrolateral medullary
ERK /
JNK /
p38 MAPK signaling in the pressor effects of
ethanol and its oxidative product
acetaldehyde . -
pubmed
METHODS : Effects of
EtOH or
ACA on blood pressure ( BP ) were evaluated in the absence or presence of selective
JNK (
SP600125 ) ,
ERK (
PD98059 ) ,
p38 (
SB203580 ) , or
ser / thr phosphatases (
okadaic acid [
OKA ] ) inhibitor . -
pubmed
SP600125 abrogated the pressor action of
EtOH , but not
ACA , thus implicating
JNK in
EtOH action on BP . -
pubmed
Despite
EtOH enhancement of
p38 phosphorylation , pharmacological studies argued against a causal role for this
kinase in
EtOH - evoked pressor response . -
pubmed
Interestingly , pharmacologic phosphatase inhibition (
OKA ) , which increased RVLM p -
ERK2 and BP , abrogated the pressor effect of subsequently administered
EtOH or
ACA . -
pubmed
CONCLUSIONS : Enhancement of RVLM
ERK2 phosphorylation constitutes a major molecular mechanism for the pressor response elicited by intra - RVLM
EtOH or its metabolite ,
ACA , in conscious SHRs . -
pubmed
Pharmacological inhibition of
GSNOR is being actively pursued as a therapeutic approach to increase
S - nitrosoglutathione levels for the treatment of
asthma and
cystic fibrosis . -
pubmed
Other than being comprised of micrometer - sized aggregates that include nanoscale particulates , Alum lacks specific physicochemical properties to explain activation of the innate immune system , including the mechanism by which
aluminum - based adjuvants engage the
NLRP3 inflammasome and
IL-1beta production . -
pubmed
Because we know that long aspect ratio nanomaterials trigger the
NLRP3 inflammasome , we synthesized a library of
aluminum oxyhydroxide (
AlOOH ) nanorods to determine whether control of the material shape and crystalline properties could be used to quantitatively assess
NLRP3 inflammasome activation and linkage of the cellular response to the material 's adjuvant activities in vivo . -
pubmed
These results demonstrate that shape , crystallinity , and
hydroxyl content play an important role in
NLRP3 inflammasome activation and are therefore useful for quantitative boosting of antigen - specific immune responses . -
pubmed
Furthermore , the transformation of
strains with
wcjE alleles from 11Av
strains was sufficient to restore partial
betaGal6OAc in an 11E background . -
pubmed
IDH2 encodes an enzyme in the
tricarboxylic acid cycle in the mitochondrion , which plays a key role in aerobic energy metabolism . -
pubmed
Single
Nucleotide Polymorphisms ( SNPs ) in genes involved in the DNA Base Excision Repair ( BER ) pathway could be associated with
cancer risk in carriers of mutations in the high - penetrance susceptibility genes
BRCA1 and
BRCA2 , given the relation of synthetic lethality that exists between one of the components of the BER pathway ,
PARP1 (
poly ADP ribose polymerase ) , and both
BRCA1 and
BRCA2 . -
pubmed
Lysyl oxidase promotes
bleomycin - induced
lung fibrosis through modulating
inflammation . -
pubmed
LOX expression is significantly upregulated in
bleomycin (
BLM)-induced
lung fibrosis , and knockdown of
LOX expression or inhibition of
LOX activity alleviates the
lung fibrosis . -
pubmed
AIM : To evaluate the efficacy and safety of
arsenic trioxide transarterial chemoembolization and intravenous administration in unresectable
hepatocellular carcinoma with lung
metastasis . -
pubmed
GPI inhibited
ADR - induced apoptosis accompanied by decreased
Fas and increased
Survivin in RA
FLS . -
pubmed
Neurotensin (
NT ) is a neurohormone produced in the central nervous system and in the gut epithelium by the enteroendocrine N cell . -
pubmed
Luminal inhibition of the
sodium - glucose cotransporter 1 (
SGLT1 ) ( by
phloridzin ) eliminated
glucose - stimulated release as well as secretion stimulated by luminal
methyl - alpha - D - glucopyranoside ( 20 % wt / vol ) , a metabolically inactive
SGLT1 substrate , suggesting that
glucose stimulates secretion by initial uptake by this transporter . -
pubmed
However , secretion was also sensitive to
GLUT2 inhibition ( by
phloretin ) and blockage of oxidative phosphorylation (
2 - 4 - dinitrophenol ) . -
pubmed
Therefore ,
glucose stimulates
NT secretion by uptake through
SGLT1 and
GLUT2 , both causing depolarization either as a consequence of
sodium - coupled uptake (
SGLT1 ) or by closure of KATP channels (
GLUT2 and
SGLT1 ) secondary to the
ATP - generating metabolism of
glucose . -
pubmed
The influence of
zinc ( II ) on
thioredoxin /
glutathione disulfide exchange : QM / MM studies to explore how
zinc ( II ) accelerates exchange in higher dielectric environments . -
pubmed
QM / MM studies were performed to explore the energetics of exchange reactions of
glutathione disulfide (
GSSG ) and the active site of
thioredoxin [
Cys32 - Gly33 - Pro34 - Cys35 ] with and without
zinc ( II ) , in vacuum and solvated models . -
pubmed
Surface morphology and porous structure of the
PSi were characterized using scanning electron microscopy ( SEM ) and transmission electron microscopy (
TEM ) . -
pubmed
The expression of
SRSF3 in
DMBA treated primary cultured oral epithelial cells were analyzed by western blot . -
pubmed
Carcinogen
DMBA treated primary cultured oral epithelial cells showed significantly increased
SRSF3 level than in control cells . -
pubmed
The Renal
Sodium Bicarbonate Cotransporter NBCe2 : Is It a Major Contributor to
Sodium and pH Homeostasis ? -
pubmed
Subsequently ,
NBCe2 was found in diverse locations where it plays a role in regulating
sodium and
bicarbonate transport , influencing intracellular , extracellular , interstitial , and ultimately plasma pH ( Boron et al . -
pubmed
NBCe2 is located in the proximal tubule Golgi apparatus under basal conditions and then disperses throughout the cell , but particularly into the apical membrane microvilli , during various maneuvers that increase intracellular
sodium . -
pubmed
Depot
medroxyprogesterone acetate use associated with increased
hemoglobin and immune activation (
HBD ,
HBB ,
IL36 G ) , and decreased epithelial repair proteins (
TFF3 ,
F11R ) . -
pubmed
Two advantages of the new procedure are : ( a ) no
benzyl ester was formed and ( b ) a stereoisomeric mixture of
syn - and anti - alcohols at the beginning of the synthetic sequence could be separated and the
rest of the synthesis could be carried out with the main syn - stereoisomer instead of the corresponding stereoisomeric mixture as it was the case in the previous process . -
pubmed
Fimasartan is a novel
angiotensin II receptor blocker . -
pubmed
Monitoring of the surface reaction and characterization of the adsorbed
heptacene was performed with scanning tunneling microscopy (
STM ) , X - ray photoelectron spectroscopy ( XPS ) , near - edge X - ray absorption fine structure ( NEXAFS ) spectroscopy , and density functional theory ( DFT ) calculations . -
pubmed
In the
STM images ,
heptacene shows a dumbbell shape , which may correspond to the substantial out - of - plane deformations of
heptacene on
Ag ( 111 ) . -
pubmed
The anti - aging protein
Klotho is induced by
GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells . -
pubmed
Here , we report that
GABA markedly increased circulating levels of
Klotho in
streptozotocin (
STZ)-induced
diabetes . -
pubmed
NF - kappaB activation triggers beta - cell apoptosis , and both
GABA and
Klotho suppress this pathway . -
pubmed
We found
Klotho KD augmented
NF - kappaB p65 expression , and abrogated the ability of
GABA to block
NF - kappaB activation . -
pubmed
Resveratrol can improve the dysfunction by downregulating the mast cell activation and the activity of
TGF - beta /
Wnt /
beta - catenin pathway . -
pubmed
The purpose of this study was to develop and test a nonlinear optical device to photoactivate
riboflavin to produce spatially controlled
collagen crosslinking and mechanical stiffening within the cornea . -
pubmed
A 2.3 nm long
perylene tetracarboxylic acid diimide (
PDI ) acceptor molecule equipped with
isocyanide linker groups was synthesized , adsorbed onto
Ag ,
Au and
Pt substrates , and the current - voltage ( I - V ) properties were measured by
CP -
AFM . -
pubmed
Naloxone ,
beta funaltrexamine , or
naltrindole was used with 1 muM
morphine to assess the involvement of specific
opioid receptor . -
pubmed
A
p38 mitogen - activated protein kinase (
MAPK ) activator (
anisomycin 0.1 muM ) or inhibitor (
SB 203580 , 20 muM ) was used to determine the involvement of
p38 MAPK pathway . -
pubmed
Results
Morphine decreased lipopolysaccharide and
interferon - gamma - induced microglial engulfment except the highest concentration ( 10 muM ) and both
naloxone and
naltrindole ( a selective
delta opioid receptor antagonist ) attenuated
morphine effect ( p & lt ; 0.001 ) . -
pubmed
SB203580 blocked the increased microglial engulfment induced by lipopolysaccharide and
interferon - gamma ( p & lt ; 0.001 ) ; whereas ,
anisomycin enhanced the
morphine - induced decrease of engulfment ( p & lt ; 0.001 ) . -
pubmed
This
morphine effect seems to be mediated by
delta opioid receptor and via
p38 MAPK inhibition . -
pubmed
When the
ethanol to water ratio was 10:1 , the diameter of the spherical polysaccharide nanoparticles was the smallest , and the mean particle size of the
TP - NPs ,
GLP - NPs , and
MCP - NPs was 99 + - 15 , 95 + - 7 , and 141 + - 9 nm , respectively . -
pubmed
We found that red and blue light could enhance
artemisinin accumulation by promoting the expression of the genes that were involved in
artemisinin biosynthesis , such as
amorpha-4,11 - diene synthase (
ADS ) and
cytochrome P450 monooxygenase (
CYP71AV1 ) genes . -
pubmed
18F - FDG PET /
CT Unveiling of Implant
Rupture and Clinically Unsuspected
Silicone Granuloma in Treated
Breast Cancer . -
pubmed
The
fatty acid profile ( & gt;5 % ) included
C18 : 1omega6c ( 43.1 % ) , C16 : 0 ( 14.6 % ) ,
C17 : 1omega6c ( 14.0 % ) and C14 : 0 2 - OH ( 11.1 % ) . -
pubmed
Bioflavonoids promote stable translocations between MLL -
AF9 breakpoint cluster regions independent of normal chromosomal context : Model system to screen environmental risks . -
pubmed
Overlap of MLL bcr sequences associated with both infant
acute leukemia and therapy - related
leukemia following exposure to the
topoisomerase II inhibitor
etoposide led to the hypothesis that exposure during pregnancy to biochemically similar compounds may promote infant
acute leukemia . -
pubmed
We show
bioflavonoids genistein and
quercetin most biochemically similar to
etoposide have a strong association with MLL -
AF9 bcr translocations , while
kaempferol ,
fisetin ,
flavone , and
myricetin have a weak but consistent association , and other compounds have a minimal association in both embryonic stem ( ES ) and hematopoietic stem cell ( HSC ) populations . -
pubmed
The MLL and
AF9 bcrs are sensitive to these
agents and recombinogenic independent of their native context suggesting
bcr sequences themselves are drivers of illegitimate DNA repair reactions and translocations , not generation of functional oncogenic fusions . -
pubmed
Hematoxylin and eosin staining , oil red O staining , immunohistochemistry , immunoblotting , and quantitative
polymerase chain reaction ( qPCR ) were performed for mechanistic investigations . -
pubmed
Here , we found
NGLGN3 was significantly upregulated in
neuroblastoma cells and tissues , its overexpression significantly promoted
neuroblastoma cell proliferation and growth determined by
MTT analysis , colony formation assay , cell cycle progression analysis ,
BrdU incorporation assay and animal model , while its knockdown inhibited cell proliferation and growth . -
pubmed
The
RNA polymerase I inhibitor
cx-5461 induced apoptosis , ROS production , and proliferative inhibition in the HASMCs , which was partly attenuated by
p53 knockout . -
pubmed
The specificity of
serine / threonine kinases is partly determined by interactions with a few residues near the
phospho - acceptor residue , forming the so - called
kinase - substrate motif . -
pubmed
Comparative clinical study of the efficacy of intralesional
MMR vaccine vs intralesional
vitamin D injection in treatment of
warts . -
pubmed
OBJECTIVE : To evaluate the efficacy of intralesional injection of
MMR vaccine vs
vitamin D in treatment of
warts . -
pubmed
Group A received intralesional
MMR vaccine into largest wart , and group B received intralesional
vitamin D3 into each lesion with maximum of five
warts treated in one session . -
pubmed
CONCLUSIONS : Immunotherapy by both intralesional
MMR vaccine and
vitamin D3 is simple , well - tolerated , effective , and cost - benefit modalities for the treatment of
warts . -
pubmed
In this work , we explore the electronic excitation energies of
1,4 - CHD and 1,3 -
CHD for both valence and Rydberg states by means of complete active space second - order perturbation theory ( CASPT2 ) , extended multi - state CASPT2 ( XMS - CASPT2 ) , and multiconfiguration pair - density functional theory ( MC - PDFT ) ; it is shown by comparison to experiment that MC - PDFT yields the most accurate results . -
pubmed
Parthenolide inhibits lipid accumulation via activation of
Nrf2 /
Keap1 signaling during adipocyte differentiation . -
pubmed
Furthermore ,
PL significantly reduced intracellular reactive
oxygen species ( ROS ) production during adipogenesis . -
pubmed
A total number of 5,379 proteins were identified from hemocytes of oysters after the treatments with
Ach and
ENK separately or simultaneously , and 1,475 of them were found to be significantly up - regulated , while 1,115 of them were significantly down - regulated . -
pubmed
The protein expression patterns in the groups treated by
ACh and
ENK separately were quite similar , which were dramatically different from that in the group treated by
ACh+
ENK . -
pubmed
Moreover , GO enrichment and co - expression network analyses implied that the combined immunomodulation of
ACh and
ENK might be mediated by
p53 ,
EGF - R - ErbB , and
Fc gamma R (
FcgammaR ) signaling pathways . -
pubmed
The presented protocol generates MNs within 20 days , with a success rate of 80 % , using specifically chosen molecules , such as
Sonic Hedgehog or
retinoic acid . -
pubmed
The pigment was purified and identified by mass spectrometry ( DI - EI -
MS ) and Nuclear Magnetic Resonance ( NMR ) spectroscopy as
violacein . -
pubmed
Pre- and
post -
anthracycline cardiac function and hemodynamic variables were compared within each group with paired t - tests ; the change was compared between groups using ANCOVA with adjustment for baseline values . -
pubmed
Targeting
Caveolin-1 and
Claudin-5 with
AY9944 , Improve Blood - Brain Barrier Permeability ; Computational Simulation and Experimental Study . -
pubmed
The current study aimed to determine the protective effect of
AY9944 related to
Caveolin-1 and
Claudin-5 role in lipid raft , which can rescue the blood - brain barrier from enhanced permeability . -
pubmed
Molecular docking simulation demonstrated that
AY9944 exerts a possible protective role via attenuating the interaction of the
Caveolin-1 and
cholesterol in lipid raft . -
pubmed
Rescuing blood - brain barrier from enhanced permeability via inducing the protective effect of
AY9944 related to
caveolin-1 and
claudin-5 role in lipid raft was the aim of the current study . -
pubmed
In vitro studies carried out in a
phosphate buffer saline (
PBS ) solution ( at pH 7.4 or 5.5 ) gave the maximum release levels of active substances after 10 - 240 min depending on the polymer system . -
pubmed
The increase in the level of
DON decreased the
hemoglobin concentration ( p < 0.001 ) , whereas the erythrocyte counts were only decreased at 15 mg
DON / kg feed . -
pubmed
The
enzymes show an absolute requirement for
Mn2 + as the divalent ion . -
pubmed
The
enzymes , however , differ somewhat in the pH curves as well as in the
Mn2 + and
Mg2 + concentration curves . -
pubmed
The method was found to be more sensitive to unstabilized
fibrin monomer than the serial - dilution
protamine sulfate test , or the
ethanol gel test , and detected
fibrin in a mixture of the
plasmin digestion products of
fibrin . -
pubmed
In the present study the sensitivity of the immune precipitate method for detecting specific
plasmin digestion products of
fibrin X , Y , D , and E , its sensitivity for stabilized
fibrin monomer complexes , and its sensitivity for
fibrin retaining B - peptide were measured and compared with the corresponding sensitivities of the serial - dilution
protamine sulfate test and the
ethanol gel test for detecting these same products . -
pubmed
The immune precipitate method was found to be highly sensitive to stabilized
fibrin monomer complexes and to
fibrin retaining B - peptide ; to be significantly less sensitive to the
plasmin digestion fragments X , Y , and E ; and to be insensitive to fragment D. The serial - dilution
protamine sulfate test and the
ethanol gel test were found to be insensitive to all of the
plasmin digestion products of
fibrin and less sensitive to the other
fibrin complexes . -
pubmed
After
GC analysis of the
vincamine - serum concentration the following pharmacokinetic profile could be established : The substance appears in the streaming blood with an invasion half - life of 53 min . -
pubmed
These studies suggest that the
carbohydrate intolerance of
cystic fibrosis is consequent upon an impaired
insulin response to
glucose , but that this
insulin deficiency is partly compensated for by increased peripheral tissue sensitivity to
insulin . -
pubmed
Galactose and
galactose-1 - amine were competitive inhibitors of
beta - galactosidase . -
pubmed
The stability of
neuraminidase has been found increase in the presence of
magnesium ions . -
pubmed
These cleavage fragments from
type III and IV collagens were separated from the intact
collagen chains by
SDS polyacrylamide gradient gel electrophoresis run under reducing conditions , and they were detected by their characteristic Coomassie blue staining pattern . -
pubmed
The specific binding of
125 - I - alpha - actinin to
vinculin and
metavinculin immobilized on a
polysterene surface was also demonstrated . -
pubmed
Pertussis toxin (
PT ) catalyzes the
ADP - ribosylation of several
guanine nucleotide - binding ( G ) proteins that are involved in the transduction of
cell surface receptor - mediated signals . -
pubmed
The toxin effect was blocked by heating ( 95 degrees C for 30 minutes ) , by
antitoxin antibody and was not associated with increased
cAMP levels in target cells . -
pubmed
Catalytically active immobilized dimer and monomeric forms of the
enzyme were prepared after
urea - induced dissociation of the tetramer . -
pubmed
A study of the coenzyme - binding properties of matrix - bound tetrameric , dimeric and monomeric species has shown that : ( 1 ) an immobilized tetramer binds
NAD+ with negative cooperativity , the dissociation constants being 0.085 microM for the first two coenzyme molecules and 1.3 microM for the third and the fourth one ; ( 2 ) coenzyme binding to the dimeric
enzyme form also displays negative cooperativity with Kd values of 0.032 microM and 1.1 microM for the first and second sites , respectively ; ( 3 ) the binding of
NAD + to a monomer can occur with a dissociation constant of 1.6 microM which is close to the Kd value for low - affinity coenzyme binding sites of the tetrameric or dimeric
enzyme forms . -
pubmed
Thus , the monomers of
rabbit muscle D - glyceraldehyde-3 - phosphate dehydrogenase displayed a hyperbolic kinetic saturation curve for
NAD + , whereas the tetramers exhibited an intermediary plateau region corresponding to half - saturating concentrations of
NAD+ . -
pubmed
The rate constant of inactivation due to modification of essential
arginine residues in the
holoenzyme decreased in the presence of
glyceraldehyde 3 - phosphate , probably as a result of conformational changes accompanying catalysis . -
pubmed
Angiotensin II causes sustained elevations in cytosolic
calcium in glomerulosa cells . -
pubmed
The threshold concentration for
angiotensin II - stimulated increases in cytosolic
calcium was 10(-14)-10(-13 ) M , and maximal elevation of cytosolic
calcium was produced by 10(-9 ) M
angiotensin II . -
pubmed
The
calcium signals invoked by intermediate concentrations ( 10(-12)-10(-10 ) M ) of
angiotensin II exhibited dose - dependent increases in magnitude and a gradual transition in nature between those invoked by threshold and maximal concentrations of the peptide . -
pubmed
Significant resistance against
oral T. gondii infection was induced by intraperitoneal pretreatment with
TDM but not with
MDP . -
pubmed
The apparent Km for
ATP in soluble
human adrenal glycerol kinase was 22.0 microM. -
pubmed
All D- and L - isomers of
amino acids tested were found to markedly stimulate
pepsin secretion , but to only slightly stimulate acid secretion . -
pubmed
Pepsin stimulatory response to each D and
L - isomer of amino acid was similar . -
pubmed
In contrast to the conformation in aqueous solution , the
C6 hydroxymethylene group is freely rotating around the
C6 - - C5 bond . -
pubmed
The large difference in coupling constants of the
methylene protons of
C1 ' to the
C2 ' methine proton of the
sphingosine indicates a restricted rotation around the C1'--C2 ' bond . -
pubmed
The
Ca ( 2 +)-binding constant of the C - terminal site in the
Asn-56 --> ;
Ala mutant of
calbindin D9k has been measured at seven different protein concentrations ranging from 27 microM to 7.35 mM by using
1H NMR . -
pubmed
For
calmodulin , we have measured the average
Mg ( 2 +)-binding constant of sites I and II at 0.325 , 1.08 , and 3.25 mM
protein and find a 13 - fold difference between the two extremes . -
pubmed
Specific species and tissue differences for the gene expression of
calcium - binding protein
regucalcin . -
pubmed
Addition to the dialysis perfusion solution of either
D1 - R and
D2 - R agonists , such as
SKF-38393 ( 50 and 100 microM ) and
Quinpirole ( 5 and 10 microM ) , resulted in dose - dependent increases in extracellular concentrations of GLU and
ASP , respectively . -
pubmed
The
SKF-38393 and
Quinpirole - induced effects were reduced by
SCH-23390 ( 0.5 microM ) , a
D1 - R antagonist , and by
Spiperone ( 1.0 microM ) , a
D2 - R antagonist , respectively . -
pubmed
However ,
SCH-23390 and
Spiperone did increase GLU and
ASP extracellular concentrations . -
pubmed
No differences were observed among women with primary ( no full term pregnancy ) or
secondary abortions ( some full term pregnancies ) with regard to common
HLA antigens , with both groups being homogeneous . -
pubmed
Furosemide ( 30 mg / kg ) was given orally with or without pretreatment with
derapril ( 30 mg / kg ) , an
angiotensin converting enzyme inhibitor ,
TCV-116 ( 1 mg / kg ) , an
AT1 receptor antagonist , or
losartan ( 10 mg / kg ) , another
AT1 receptor antagonist . -
pubmed
As
TCV-116 and
losartan are selective
AT1 receptor antagonists , the effect of
furosemide on renal
PGE2 production , as reflected by the urinary
PGE2 , might be mediated by an activation of
AT1 receptors . -
pubmed
Gastrin did not induce cell proliferation or increase
[ 3H ] thymidine incorporation and no high - affinity
gastrin binding sites were observed . -
pubmed
An assay to detect
glycoproteins that contain
mannose 6 - phosphate . -
pubmed
We have developed a broadly applicable solid phase assay for quantitative and qualitative analysis of phosphorylated lysosomal enzymes and other
glycoproteins that contain
mannose 6 - phosphate (
Man6 - P ) residues . -
pubmed
Samples are immobilized on membranes and proteins that contain
Man6 - P are detected using an iodinated , soluble form of the
cation - independent Man6 - P receptor . -
pubmed
Second , it can be used in a western blot format to identify
Man6 - P glycoproteins that have been fractionated by
polyacrylamide gel electrophoresis . -
pubmed
No correlation could be found between
sE - selectin concentrations and
Gd enhancement on MRI , but a close correlation existed between mean concentrations of
sE - selectin and
TNF alpha ( r = 0.71 , P & lt ; 0.001 ) . -
pubmed
Raised
CRP concentrations did correlate with infectious episodes , clinical
relapse , and
Gd enhancement , and were significantly raised when no MRI activity was found . -
pubmed
The expression of
aromatase by
breast cancer cells and the role of locally produced
estrogen in the stimulation of
tumor growth has been controversial . -
pubmed
The functional significance of locally produced
estrogens on growth of the
tumor was addressed by measuring
aromatase activity and a marker of proliferation (
PCNA score ) . -
pubmed
In the ten
tumors that showed immunoreaction to
aromatase , the average
aromatase activity measured in cryosections was 286.5 + /- 18.6 (
SE ) fmol
estrogen / mg protein.h , whereas in nine
tumors with weak
aromatase immunoreaction , the enzyme activity was 154.7 + /- 19.3 (
SE ) fmol
estrogen / mg protein - h ( P & lt ; 0.05 ) . -
pubmed
In two of these
tumors ,
aromatase activity was high and
[ 3H ]- thymidine incorporation into DNA was also stimulated by
testosterone . -
pubmed
In the other two
tumors that had low
aromatase activity , no such stimulation occurred with
testosterone . -
pubmed
The results indicate that
aromatase is expressed mainly in
tumor epithelial cells and that sufficient amounts of
estrogen are synthesized by the
tumor to produce a proliferative response . -
pubmed
We demonstrate that binding studies using
[ 125I ] HPP - HOE140 offer high sensitivity and specificity for characterization , quantitation and localization of the
bradykinin B2 receptors in tissues and offers new information on uterine
bradykinin B2 receptors . -
pubmed
13
RA , 23
osteoarthritis (
OA ) , 21
joint trauma (
JT ) , and 9
synovial sarcoma (
SS ) specimens were immunostained for
Ki-67 and
PCNA ,
silver - stained for nucleolar organizer regions ( AgNORs ) , and double stained with either T - cell- (
CD3 ) , macrophage- (
CD68 ) , or polymorphonuclear neutrophilic leucocytes ( PMN)-markers (
CD15 ) . -
pubmed
The mean number of abnormal psychometric test results and the prevalence of
SHE were used for a quantitative evaluation of the efficacy of the
lactulose treatment . -
pubmed
Treatment patterns and distribution of
low - density lipoprotein cholesterol levels in treatment - eligible United States adults . -
pubmed
To estimate the fraction of United States ( U.S. ) adults who are eligible for treatment to reduce elevated
low - density lipoprotein (
LDL )
cholesterol levels based on Adult Treatment Panel
II ( ATP
II ) guidelines and the percent reduction in
LDL cholesterol required by those who qualify for treatment , we analyzed data on 7,423 respondents to Phase 2 of the third National Health and Nutrition Examination Survey ( NHANES III ) administered between 1991 and 1994 . -
pubmed
Eighty - two percent of adults with
coronary heart disease are not at their target
LDL cholesterol level of 100 mg / dl . -
pubmed
Overall , 40 % of people who qualify for drug therapy require an
LDL cholesterol reduction of & gt ; 30 % to meet their ATP
II treatment goal . -
pubmed
Approximately 75 % of those with
coronary heart disease who qualify for drug therapy require an
LDL cholesterol reduction of & gt;30 % . -
pubmed
Although elevated
LDL cholesterol levels can be treated , prevalence rates in the U.S. adult population remain high . -
pubmed
The findings in this study provide at least a partial explanation for why many patients receiving therapy do not reach their treatment goals : they require a larger reduction in
LDL cholesterol than many therapies can provide . -
pubmed
The first reaction binds
UDP -
glucose (
UDP - Glu ) , displaces
glucose-1 - phosphate (
glu-1 - P ) , and forms the
UMP -
GALT intermediate . -
pubmed
In the second reaction ,
galactose-1 - phosphate (
gal-1 - P ) is bound ,
UDP - galactose (
UDP - Gal ) is released , and the free
enzyme is recycled . -
pubmed
Gln188 -
GALT displaced 80 + /- 7 . -
pubmed
By contrast , both
Arg188- and
Asn188 -
GALT released more glu-1
- P ( 170 + /- 8.0 and 129 + /- 28.4 nmol / mg GALT / min , respectively ) . -
pubmed
Trials were reviewed that compared
calcitonin with placebo , no therapy , or
calcium with or without
vitamin D , and that mentioned
fracture as an outcome . -
pubmed
The fungus - inducible
PepThi gene is highly inducible only in the unripe fruit by
salicylic acid . -
pubmed
Expression of the
jl - l gene is enhanced by
jasmonic acid in the unripe fruit but suppressed in the ripe fruit . -
pubmed
The amount of
phosphorous in the
protein increased with the excess of
phosphorous oxychloride and the pH of reaction . -
pubmed
The bulk of
phosphorus was only loosely bound to the
protein and was removed by washing with cold
perchloric acid . -
pubmed
The more stably bound
phosphorus groups after reaction at pH 8 were found to be nearly equally attached to
amino and
hydroxyl groups , whereas phosphorylation at pH 10 - 11 led to predominant O - phosphorylation as detected by studying the acid- and alkali - lability of the
protein -
phosphorous bonds . -
pubmed
Similar losses in hepatic enzyme were observed for
benzyloxy - resorufin and
pentoxy - resorufin dealkylase (
CYP2B ) and
chlorzoxazone hydroxylation (
CYP2E ) . -
pubmed
We conclude that
N - OH - PABA decreases
NAT1 activity by a direct interaction with the enzyme and appears to be a result of covalent modification at the cofactor - binding site . -
pubmed
This is in contrast to our findings for
PABA , which appears to reduce
NAT1 activity by down - regulating the enzyme , leading to a decrease in
NAT1 protein content . -
pubmed
Urinary excretion of
proANP peptides significantly increased , particularly when the serum
creatinine level was> ; 5.0 mg / dL [
proANP(1 - 30 ) 26 - fold ,
proANP(31 - 67 ) 8.4 - fold ] . -
pubmed
It is proposed that the diseased kidney increases early urinary excretion of
proANP fragments to participate in the regulation of renal function as well as
sodium and water excretion . -
pubmed
tuberculosis
antigens was observed between the
DRB1 * 1501 , * 1502 and * 1503 positive or negative controls , ATB and
ITB patients . -
pubmed
Amounts of
ADAM15 protein ( 102+/-12 versus 40+/-6 U ; P<0.01 ) and mRNA ( 24.0+/-5.6 versus 10.5+/-2.5 U ; P<0.05 ) increased significantly during
AF compared with
sinus rhythm . -
pubmed
Causes of interscan variability of coronary artery
calcium measurements at electron - beam
CT . -
pubmed
RATIONALE AND OBJECTIVES : The authors performed this study to investigate the causes of interscan variability of coronary artery
calcium measurements at electron - beam computed tomography (
CT ) . -
pubmed
Indeed , we show that fluid intake had a significant impact :
albumin concentration decreased , whereas the
albumin /
creatinine ratio ( A / C ) and
albumin excretion rate increased ( P<.001 ) significantly . -
pubmed
Following 12 weeks of
diabetes , there was a small increase in the level of
glutamine synthetase labelling in
somata and endfeet compared with controls ( ANOVA , P & lt ; 0.05 ) . -
pubmed
ACBP and
cholesterol differentially alter
fatty acyl CoA utilization by microsomal
ACAT . -
pubmed
Microsomal
acyl CoA : cholesterol acyltransferase (
ACAT ) is stimulated in vitro and/or in intact cells by proteins that bind and transfer both substrates ,
cholesterol , and
fatty acyl CoA. -
pubmed
To resolve the role of
fatty acyl CoA binding independent of
cholesterol binding / transfer , a protein that exclusively binds
fatty acyl CoA (
acyl CoA binding protein ,
ACBP ) was compared . -
pubmed
In the presence of exogenous
cholesterol ,
ACAT was stimulated in the order :
ACBP & gt ;
sterol carrier protein-2 (
SCP-2 ) & gt ;
liver fatty acid binding protein (
L - FABP ) . -
pubmed
In contrast , in the absence of exogenous
cholesterol , these proteins inhibited microsomal
ACAT , but in the same order :
ACBP & gt ;
SCP-2 & gt ;
L - FABP . -
pubmed
The extracellular protein BSA stimulated microsomal
ACAT regardless of the presence or absence of exogenous
cholesterol . -
pubmed
Thus ,
ACBP was the most potent intracellular
fatty acyl CoA binding protein in differentially modulating the activity of microsomal
ACAT to form
cholesteryl esters independent of
cholesterol binding / transfer ability . -
pubmed
The
enzyme showed a high
dioxygenase activity only for
1,2,4 - trihydroxybenzene , with K(m ) and V(max ) values of 9.6 micro M and 6.8 micro mol min(-1 ) mg of
protein(-1 ) , respectively . -
pubmed
OBJECTIVE : To evaluate the short - term bone fusion after implantation of composite biosynthetic bone made of
coralline hydroxyapatite composite (
CHC ) as a substitute for autologous bone grafting . -
pubmed
[ The effect of
diphenylmethane derivatives and of a basic
alkyl derivative of
o -( 4 - hydroxybenzoyl )- benzoic acid methyl ester on the uterine muscle and on the action of
hypophysin ] . -
pubmed
The
tritium rate effect in
tyrosinase - catalyzed hydroxylation . -
pubmed
Involvement of hepatic
glucocorticoid receptor - mediated functions in
steroid - induced
cataract formation . -
pubmed
Determination of whether the
steroid - induced
cataract formation is caused through
glucocorticoid ( GC ) receptor - mediated process was conducted by using GC antagonist (
RU486 ) and anti -
GC receptor antibody , and by
sucrose density gradient ultracentrifugation analysis . -
pubmed
( 1 ) When 15 day - old chick embryos were treated with
dexamethasone (
DEX , 0.025 micromol per egg ) , their lenses started to form an opaque ring around the peri - nuclear region ( stage
II - III ) after 12 hr and developed into nuclear - like
cataract ( stage
IV - V ) after 44 hr . -
pubmed
( 3 )
Sucrose gradient ultracentrifugation analysis indicated that the receptor (
9S ) in the liver could be transformed to the 4S form after 0.4 M
NaCl treatment . -
pubmed
The
Gr5a gene on the X chromosome , which encodes a receptor for the
sugar trehalose , has transposed from one such tandem cluster of six genes at cytological location 64 , as has
Gr61a , and all eight of these receptors might bind
sugars . -
pubmed
The differential effects of
angiotensin II receptors inhibitors
losartan and
PD123319 on the
pancreatic injury were assessed by virtue of using the pancreatic water content , biochemical and histological analyses . -
pubmed
Blockade of the
AT ( 1 ) receptor by
losartan at a dose of 200microg / kg could markedly ameliorate the
pancreatic injury induced by cerulein , as evidenced by biochemical and histopathological studies . -
pubmed
However , blockade of the
AT ( 2 ) receptor by
PD123319 appeared not to provide any beneficial role in
cerulein - induced
pancreatic injury . -
pubmed
The protective action of
losartan was linked to an inhibition of
NAD ( P ) H oxidase activity , thus consequential oxidative modification of pancreatic proteins in the pancreas . -
pubmed
Thus the protective role of
AT ( 1 ) receptor antagonist ,
losartan , could be mediated by the inhibition of
NAD ( P ) H oxidase - dependent generation of reactive
oxygen species ( ROS ) . -
pubmed
At
MRS ,
cancer patterns were diagnosed when the ratio of
choline plus
creatine to
citrate was greater than 0.86 . -
pubmed
[ Studies on
alkaline phosphatases in
osteoarticular tuberculosis and effect of
streptomycin therapy ] . -
pubmed
At the dose of 50 mg / kg ,
riparin III increased the number of entries in the open arms of the
EPM test as compared with control . -
pubmed
The subunit that contains the
sulfate ( or
phosphate ) ion and two
magnesium ions is in the closed conformation observed in
enolase complexes with the substrate or its analogues ; the other subunit is in the open conformation observed in
enolase subunits without bound substrate or analogues . -
pubmed
Although
fibric acid derivatives increase serum
HDL - C levels and decrease
triglyceride levels , their effects on renal function are largely unknown . -
pubmed
During positive ion LC / ESI -
MS , LG formed an abundant sodiated species ( [ M+
Na]+ ) while the protonated molecule was barely observable . -
pubmed
Neither antibody blocking procedures nor an
avidin /
biotin block were entirely satisfactory for eliminating this background staining . -
pubmed
Prophenin-2 (
PF-2 ) is a
cathelicidin ,
97 - amino - acid antimicrobial protein stored in neutrophil secondary granules . -
pubmed
CONCLUSIONS : The treatment intensity achieved with
PEG ASNase in the present study appears to be acceptable based on the surrogate of serum
ASNase activity . -
pubmed
Treatment of leaves with
butanedione monoxime , an inhibitor of
myosin ATPase , resulted in similar behaviour of mitochondria to that under ROS stress . -
pubmed
It seems that
neutrophil migration impairment is mediated by the circulating inflammatory
cytokines , such as
TNF - alpha and
IL-8 , which induce the
nitric oxide (
NO ) production systemically . -
pubmed
In conclusion , we demonstrated that , in
sepsis , failure of
neutrophil migration is critical for the outcome and that
NO is involved in the process . -
pubmed
The method is based on derivatization of JA with
5 - bromomethylfluorescein (
5 - BMF ) and separation and quantification of the resulting
5 - BMF - JA derivative by capillary electrophoresis coupled to laser - induced fluorescence detection ( CE -
LIF ) . -
pubmed
Expression of the
collagen type II gene was selectively upregulated for PGA , PGCL , and
PLGA and slightly increased for P(LA - CL)25 polymers but was downregulated for
fullerene C-60 DMA . -
pubmed
Aggrecan gene expression was strongest with
PGA and was consistently expressed with other matrices , especially with PGCL and
PLGA . -
pubmed
We found the relationship between the 3 ' end
ACCN sequences of
tRNA molecules and the activated
amino acids and its biological significance , the rRNAs ' consensus sequences 5'NCC ... TGG3 ' or 5'TGG ...
NCC3 ' which may perform as functional segments of rRNAs to help triggering the reaction of peptide formation , and common nature of releasing factors that the first
amino acid residue of releasing factors
ERF ,
RF1 and
RF2 are all
Methionine , except a few
Alanine , which may be necessary for releasing the translated polypeptide and stopping the translating process . -
pubmed
There was an increase in the levels of
fructose 1,6 - P ( 2 ) ,
sedoheptulose 1,7 - P ( 2 ) ,
triose - P ,
ribose 5 - P , and
ribulose 5 - P pools , indicating inactivation of stromal enzymes , most notably
fructose 1,6 - bisphosphatase ,
sedoheptulose 1,7 - bisphosphatase , and
ribulose 5 - P kinase . -
pubmed
The mechanisms of
inflammation include cellular
peroxide production , acidic solutions ,
glucose and its degradation products , and the integrated response of the cellular -
cytokine cascade . -
pubmed
Cardiovascular and antiinflammatory therapeutics may interfere with
TGF and thereby influence the effect of
thiazides once co - administration is clinically indicated . -
pubmed
Similar results were obtained with the
AT II type 1 receptor antagonist
candesartan ( 8 mg ): GFR remained stable ,
Na (+ ) excretion rose by 35 % and
K (+ ) excretion was not changed . -
pubmed
The effect of the
Ca ( 2 + ) channel blocker
amlodipine ( 5 mg ) on
GFR and HCT - induced
Na (+ ) excretion equalled that obtained with the
AT ( 1 ) blocker , yet with this treatment
K (+ ) excretion rose in proportion to
Na (+ ) excretion . -
pubmed
The beta - blockers
propranolol ( 80 mg ) or
bisoprolol ( 5 mg ) reduced
GFR but maintained
TGF . -
pubmed
The inhibition of
cyclooxygenase by
diclofenac ( 50 mg ) or
rofecoxib ( 25 mg ) significantly reduced the diuretic / natriuretic effect of HCT , but
K (+ ) excretion was unchanged , and
TGF was still demonstrable . -
pubmed
CONCLUSION : In conclusion ,
AT ( 1 ) receptors , as well as the
Ca ( 2 + ) channels in the smooth muscle cells of the afferent arteriole , are considered prerequisites for
TGF function ; their blockade increases the diuretic / natriuretic efficacy of
thiazide diuretics . -
pubmed
Resveratrol binds to the
sulfonylurea receptor (
SUR ) and induces apoptosis in a
SUR subtype - specific manner . -
pubmed
Sulfonylurea receptors (
SURs ) constitute the regulatory subunits of
ATP - sensitive K + channels ( K(ATP ) channels ) . -
pubmed
Optimization of
halopemide for
phospholipase D2 inhibition . -
pubmed
Halopemide , which was identified by HTS to inhibit
phospholipase D2 (
PLD2 ) , provided the basis for an exploratory effort to identify potent inhibitors of
PLD2 for use as inflammatory mediators . -
pubmed
Parallel synthesis and purification were utilized to rapidly identify orally available
amide analogs derived from
indole 2 - carboxylic acids with superior potency versus
PLD2 . -
pubmed
Site - directed mutagenesis analysis demonstrated that the GxGK(232)T to GxGAT mutation in the conserved motif I abolished the
NTPase activity of
ntNS3 , whereas substitution of
TATPA(354 ) for TATPV in the motif III had no effect on the enzyme activity . -
pubmed
Strychnine - sensitive
glycine receptors mediate the analgesic but not hypnotic effects of emulsified volatile anesthetics . -
pubmed
These results suggest that
strychnine - sensitive
glycine receptors may contribute to the analgesic but not to the hypnotic effects induced by
ether ,
enflurane ,
isoflurane and
sevoflurane . -
pubmed
In recent years , the molecular structures of
Ngb with
carbon monoxide bound to the
heme iron and without an exogenous ligand have been solved , and interesting structural changes have been noticed upon ligand binding . -
pubmed
A decrease in intracellular
magnesium , caused by
magnesium deficiency , releases the
magnesium - mediated inhibition of
ROMK channels and increases
potassium secretion . -
pubmed
Heparin attenuates
metastasis mainly due to inhibition of
P - and L - selectin , but non - anticoagulant heparins can have additional effects . -
pubmed
The virtual elimination of
metastasis by a single treatment with a modified heparin without anticoagulant activity strongly suggests that
heparin primarily reduces
metastatic disease by inhibiting
P - and L - selectin interactions . -
pubmed
An important regulatory mechanism of this complex is through acetylation and
SIRT1 - mediated
lysine de - acetylation under low nutrient conditions . -
pubmed
RESULTS : Increased uptake levels of
18F - FDG were observed in the esophagi after
EDA procedure . -
pubmed
Moreover , the
adenosine receptor antagonist ,
caffeine , potently attenuates the EEG markers of nonREM sleep homeostasis during sleep , as well as during wakefulness . -
pubmed
While these convergent findings strongly support an important contribution of
adenosine and
adenosine receptors to nonREM sleep homeostasis , further research is needed to elucidate the underlying mechanisms that mediate the actions of
adenosine on sleep and the sleep EEG . -
pubmed
The biologic agent
bevacizumab (
Avastin ) , an inhibitor against
vascular endothelial growth factor , and
cetuximab (
Erbitux ) and panitumumab (
Vectibix ) , monoclonal antibodies to
epidermal growth factor receptor , have demonstrated their efficacy in clinical trials . -
pubmed
In the present study , we show that
ISG15 is a determinant for
CPT sensitivity / resistance possibly through its effect on proteasome - mediated repair of
topoisomerase I (
TOP1)-DNA covalent complexes . -
pubmed
Second , the level of
ISG15 was found to be significantly reduced in several
tumor cells selected for resistance to
CPT , suggesting that altered
ISG15 regulation could be a significant determinant for acquired
CPT resistance . -
pubmed
Taken together , these results suggest that
ISG15 , which interferes with proteasome - mediated repair of
TOP1 - DNA covalent complexes , is a potential
tumor biomarker for
CPT sensitivity . -
pubmed
Posttranslational
carboxyl methylation of
small GTPases , such as
RhoA and
Ras , regulates subcellular localization and
GTPase activity , resulting in altered cellular function . -
pubmed
In this study , we investigated the role of
RhoA carboxyl methylation in modulating endothelial monolayer permeability . -
pubmed
These results suggest that
ICMT modulates endothelial monolayer permeability by altering
RhoA carboxyl methylation and activation . -
pubmed
The
APOE gene was found to be of bimodal structure , with a hypomethylated
CpG - poor promoter and a fully methylated 3'-CpG - island , that contains the sequences for the epsilon4 - haplotype , which is the only undisputed genetic risk factor for
LOAD . -
pubmed
The concentrations of
cytokines [
interleukin ( IL )- 4 ,
IL-10 ,
IL-8 ,
IL-6 ,
tumor necrosis factor - alpha (
TNFalpha ) ,
IL-1beta ,
interferon - gamma ) were assessed by ELISA , and
nitric oxide (
NO ) production was evaluated by Griess assay . -
pubmed
Arg(high)/
Gln(high ) decreased the production of
TNFalpha ,
IL-1beta ,
IL-8 , and
IL-6 ( each P & lt ; 0.01 ) . -
pubmed
Arg(low)/
Gln(high ) decreased
IL-6 and
IL-8 production ( both P & lt ; 0.01 ) , whereas
Arg(high)/
Gln(low ) did not affect
cytokine and
NO production . -
pubmed
Arg(low)/
Gln(high ) and
Arg(high)/
Gln(high ) decreased
NF - kappaB p65 subunit expression , whereas
p38 MAPK was decreased only by
Arg(high)/
Gln(high ) . -
pubmed
Combined pharmacological doses of
Arg and
Gln decreased
TNFalpha and the main proinflammatory
cytokines release in active colonic
CD biopsies via
NF - kappaB and
p38 MAPK pathways . -
pubmed
We then administered physiologic doses of
thyroxine to inhibit
thyroid - stimulating hormone secretion . -
pubmed
Mutagenesis of key
arginine residues in the DNA contact sequence results in the loss of specific interaction of the PEST sequence with the
p65 subdomain . -
pubmed
We conclude that the local structure of the
IkappaBalpha / NF - kappaB complex in the region of the PEST sequence is consistent with a direct interaction of this
acidic sequence with the basic DNA contact sequence in
p65 , thus reducing the affinity of
NF - kappaB for DNA by a competitive mechanism that is still to be elucidated fully . -
pubmed
Selective
estrogen receptor modulators ( SERMs ) , such as
tamoxifen (
TAM ) , have been used extensively for the treatment and prevention of
breast cancer and other pathologies associated with aberrant
estrogen receptor (
ER ) signaling . -
pubmed
However , the observation that SERMs can also regulate aspects of
calcium signaling and apoptosis in an
ER - independent manner in some systems suggests that some of the activity of drugs within this class may also arise as a consequence of their ability to interact with targets other than
ER . -
pubmed
In this study , we demonstrate that
4 - hydroxy - TAM (
4OHT ) , an active metabolite of
TAM , directly binds to and modulates the transcriptional activity of the
aryl hydrocarbon receptor (
AHR ) . -
pubmed
Of specific interest was the observation , that in the absence of
ER ,
4OHT can induce the expression of
AHR target genes involved in
estradiol metabolism , cellular proliferation , and
metastasis in cellular models of
breast cancer . -
pubmed
The potential role for
AHR in
SERM pharmacology was further underscored by the ability of
4OHT to suppress osteoclast differentiation in vitro in part through
AHR . -
pubmed
Cumulatively , these findings provide evidence that it is necessary to reevaluate the relative roles of
ER and
AHR in manifesting the pharmacological actions and therapeutic efficacy of
TAM and other SERMs . -
pubmed
Further studies showed that
RES also inhibited
H ( 2 ) O ( 2 ) induced
p38 and
JNK phosphorylation . -
pubmed
As
oxygen concentrations in the
UGT are low under physiological conditions ( O(2 ) & lt ; 5 % ) and further decrease during an inflammatory process , we wondered whether antibacterial properties of
IFN - gamma are maintained under hypoxic conditions . -
pubmed
Impaired effectiveness of
IFN - gamma on chlamydial growth under
hypoxia was accompanied by reduced phosphorylation of
Stat-1 on
Tyr701 and diminished
IDO activity . -
pubmed
trachomatis depend on the environmental
oxygen supply , which could explain inadequate bacterial clearance and subsequent
chronic infections eventually occurring in the
UGT of women . -
pubmed
TBADH was active at a water concentration & gt;=10 % v / v. In the reduction reaction of
sulcatone to
sulcatol carried out in biphasic systems , the yield obtained with
SBA-15 - adsorbed TBADH was up to 5.5 - fold higher than that with the free
enzyme , which suggests a higher stability of the immobilized
enzyme toward the organic solvent . -
pubmed
AIMS : To ( i ) determine whether
methylarginine - specific antibodies can be employed for standard immunohistochemical analysis of paraffin - embedded tissues , ( ii ) analyse
methylarginine expression in
normal and
neoplastic tissues and ( iii ) correlate
methylarginine expression with that of
protein arginine methyltransferase (
PRMT1 ) , the predominant cellular
arginine methyltransferase . -
pubmed
METHODS AND RESULTS : Immunohistochemistry of
normal and
cancer tissues was performed utilizing three commercial polyclonal antibodies : anti -
methylarginine - specific antibody ( anti -
mRG ) raised against a
methylarginine peptide , Control antibody ( anti -
RG ) , a control antiserum raised against a corresponding
arginine peptide without any methylated residues and anti -
PRMT1 . -
pubmed
A correlation between
methylarginine staining and
PRMT1 expression was found in all
normal and
cancer tissues analysed . -
pubmed
CONCLUSION :
Methylarginine - specific antibodies are capable of recognizing
methylarginine proteins (
MeRP ) in paraffin - embedded tissues . -
pubmed
The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of
methylarginine proteins (
MeRP ) in biological processes including
carcinogenesis . -
pubmed
)-induced elevation of plasma
catecholamines was reduced by
dihydro - beta - erythroidine ( a selective antagonist of
alpha4beta2 nicotinic acetylcholine receptors ) ( 100 and 300nmol / animal , i.c.v . ) -
pubmed
, while
methyllycaconitine ( a selective antagonist of
alpha7 nicotinic acetylcholine receptors ) ( 100 and 300nmol / animal , i.c.v . ) -
pubmed
RJR-2403 ( a selective agonist of
alpha4beta2 nicotinic acetylcholine receptors ) ( 2.5 and 5mumol / animal , i.c.v . ) -
pubmed
elevated plasma
noradrenaline and
adrenaline (
adrenaline>
noradrenaline ) , while
PNU-282987 ( a selective agonist of
alpha7 nicotinic acetylcholine receptors ) ( 2.5 and 5mumol / animal , i.c.v . ) -
pubmed
Increased levels of ROS ,
MDA and
CD4(+ ) lymphocyte apoptosis were positively associated with disease activity while decreased levels of
GSH and percentage expression of
CD4(+ ) lymphocyte were negatively associated with disease activity . -
pubmed
The decrease in intracellular levels of
GSH was negatively associated with T lymphocyte ,
CD4(+ ) lymphocyte ,
CD8(+ ) lymphocyte apoptosis and intracellular
caspase-3 expression . -
pubmed
TEM , SEM and EDX analyses indicated that
Ag nanoparticles were successfully deposited onto the surfaces of hollow
SiO ( 2 ) beads . -
pubmed
Noticeably ,
ginsenoside Rg(6 ) ( 12 ) and
ginsenoside F(4 ) ( 13 ) exhibited particularly inhibitory effect on LPS - induced
IL-12 production with the inhibition values of 80 and 82 % ; and
ginsenoside ST(1 ) ( 4 ) ,
ginsenoside SL(2 ) ( 8) ,
ginsenoside SL(3 ) ( 9 ) ,
ginsenoside Rh(3 ) ( 14 ) ,
ginsenoside Rk(2 ) ( 15 ) , and
ginsenoside Rs(4 ) ( 18 ) showed moderate effects with inhibition rates of 63 , 65 , 67 , 68 , 71 , 73 , and 67 % , respectively . -
pubmed
The results show that the
amino - acid distribution probability representing the primary structure of
cellulase can predict both optimal pH and temperature , whereas various properties of
amino acids related to the primary structure can not do so . -
pubmed
RESULTS :
Diazoxide inhibited in vitro
nitric oxide (
NO ) ,
tumor necrosis factor alpha (
TNF - alpha ) and
interleukin-6 (
IL-6 ) production and
inducible nitric oxide synthase (
iNOS ) expression by activated microglia without affecting
cyclooxygenase-2 (
COX-2 ) expression and phagocytosis . -
pubmed
Diazoxide did not affect the number of infiltrating lymphocytes positive for
CD3 and
CD20 in the spinal cord . -
pubmed
In a multicenter randomised 2 x 16 - week cross - over study ( n = 86 ) , with a 6 - week wash out period preceding and between treatment periods , intravenous
alfacalcidol and
paricalcitol were given by forced titration ( 50 % dose increase ) every second week , until
parathyroid hormone were sufficiently suppressed or ionised
calcium and/or
phosphate levels were elevated . -
pubmed
FGF23 increases renal
phosphate excretion and decreases levels of
1,25 - dihydroxyvitamin D. -
pubmed
We explored the influence of
alfacalcidol and
paricalcitol on
FGF23 in stored blood samples from the beginning and the end of each treatment period . -
pubmed
FGF23 increased significantly and equally during treatment with
alfacalcidol and
paricalcitol . -
pubmed
Furthermore , we found baseline
FGF23 to predict
PTH levels after 16 weeks of
vitamin D analog treatment . -
pubmed
gamma - Aminobutyric acid type A (
GABA ( A ) ) receptors are principal targets for most anesthetics . -
pubmed
The second aim of the study was to determine whether
memory deficits after
isoflurane can be reversed by inhibiting
alpha5GABA ( A ) receptors . -
pubmed
We also sought to determine whether the expression of
alpha5GABA ( A ) receptors is necessary for the development of
memory dysfunction after
isoflurane . -
pubmed
Gastric bleeding was not induced by
AAD-2004 even at a dose 400 - fold higher than that required to obtain maximal therapeutic efficacy in
superoxide dismutase 1(G93A ) . -
pubmed
Targeting both
mPGES-1 - mediated
PGE ( 2 ) and free radicals may be a promising approach to reduce neurodegeneration in
ALS and possibly other
neurodegenerative diseases . -
pubmed
The increased
cytotoxicity was associated with a concurrent increase in the number of apoptotic cells and production of reactive
nitrogen species (
RNS ) . -
pubmed
This can delay subsequent use of
radioactive iodine (
RAI ) therapy because the
iodine can compete for uptake . -
pubmed
Since its discovery in 1995 identifying the
NAADP receptor protein / Ca2 + channel has been a major persuit of the
Ca2 + signalling community . -
pubmed
In their paper ' The N - terminal region of two - pore channel 1 regulates trafficking and activation by
NAADP ' published in this issue of the Biochemical Journal Patel and colleagues describe that the N - terminus of one of the
NAADP receptor protein /
Ca2 + channel candidates ,
TPC1 ( two - pore channel 1 ) , is crucial for protein targeting and for sensitivity to
NAADP . -
pubmed
We characterized the binding of GAG heparin
octasaccharide to
dCXCL1 using solution NMR spectroscopy . -
pubmed
Purine derivatives as potent
Bruton ' s tyrosine kinase (
BTK ) inhibitors for
autoimmune diseases . -
pubmed
Investigation of various
heterocyclic core isosteres of
imidazopyrazines 1 & amp ; 2 yielded
purine derivatives 3 & amp ; 8 as potent and selective
BTK inhibitors . -
pubmed
Subsequent
SAR studies of the
purine series led to the discovery of 20 as a leading compound . -
pubmed
Biochemical investigations with recombinant proteins demonstrated that
L - tryptophan and derivatives , including
D - tryptophan ,
4 - , 5- , 6- and
7 - methyl - dl - tryptophan , were well accepted by both
enzymes in the presence of
DMAPP . -
pubmed
Structural elucidation of the isolated products revealed regiospecific prenylation at C-6 of the
indole ring and proved unequivocally the identification of two very similar
6 - dimethylallyltryptophan synthases (
6 - DMATS ) . -
pubmed
Utilizing
GPP as a
prenyl donor has not been reported for
tryptophan prenyltransferases previously . -
pubmed
Study variables were
tumor weight (
TW ) ;
Hb , as index of
tumor - mediated angiogenesis ;
vascular endothelial growth factor (
VEGF ) protein abundance ; protein
carbonyl content ;
alpha - tubulin abundance ; and
GluAp ,
AlaAp , and
AspAp activities . -
pubmed
BSO produced a major decrease in
TW ( 203 + - 18 mg ) with respect to controls ( 365 + - 26 ) and a reduction in
Hb content . -
pubmed
The
TETRAC group also showed marked reductions in
TW ( 129 + - 15 ) and
Hb concentration associated with a reduced
VEGF content . -
pubmed
BSO treatment increased protein
carbonyl and
tubulin abundance in comparison to controls . -
pubmed
In conclusion , administration of
BSO reduced the
TW , which inversely correlated with protein
carbonyl content , suggesting a loss of microtubule polymerization . -
pubmed
Class I - specific
histone deacetylase inhibitor
MS-275 overrides
TRAIL - resistance in
melanoma cells by downregulating
c - FLIP . -
pubmed
Conversely , class II - selective HDACi
MC1575 has shown no effect on the resistance of
melanoma cells and was able exclusively to increase
TRAIL - induced cell
death in responsive cells . -
pubmed
However , only
MS-275 markedly decreased the expression levels of both the long and short
c - FLIP isoforms . -
pubmed
RNAi - mediated
c - FLIP silencing resulted in
caspase 8 - dependent apoptosis in survivor cells which was comparable to that observed following
MS-275 treatment . -
pubmed
Accordingly , enforced expression of ectopic
c - FLIP has abolished the cooperative induction of apoptosis by the combination of
MS-275 and
TRAIL . -
pubmed
Inhibition of
class I HDAC isoenzymes 1 , 2 and 3 has resulted to be functionally important for
c - FLIP downregulation by
MS-275 . -
pubmed
In contrast , knockdown of
class II HDACs has had no effect on
c - FLIP expression , thus explaining the dual incapacity of
MC1575 to inhibit
c - FLIP expression and sensitize cells resistant to
TRAIL . -
pubmed
The data reported here suggest that
MS-275 represents a promising therapeutic approach in combination with
TRAIL for treatment of
cutaneous and uveal melanoma due to its ability to reduce
c - FLIP expression . -
pubmed
In the follow - up arm , a multivariable analysis adjusted for age , birth year ,
BMI , education , socioeconomic status , ethnicity , and physical activity yielded hazard ratios of 0.32 ( 95 % CI 0.10 - 0.82 , P = .002 ) for incident
obesity , 0.49 ( 95 % CI 0.25 - 0.95 , P = .037 ) for incident
triglycerides 150 mg / dL or greater , 0.56 ( 95 % CI 0.31 - 0.98 , P = .048 ) for
low - density lipoprotein cholesterol 130 mg / dL or greater , and 2.14 ( 1.368 - 3.359 , P = .001 ) for
high - density lipoprotein cholesterol less than 40 mg / dL for
FMF participants compared with controls . -
pubmed
Hesperetin Induces Apoptosis in
Breast Carcinoma by Triggering Accumulation of ROS and Activation of
ASK1 /
JNK Pathway . -
pubmed
The
cytotoxic effect of
hesperetin was due to the induction of apoptosis as evident from the
phosphatidyl - serine externalization , DNA fragmentation ,
caspase-7 activation , and
PARP cleavage . -
pubmed
Pre - treatment with
caspase-9 specific inhibitor (
Z - LEHD - fmk ) markedly attenuated apoptosis suggesting an involvement of intrinsic mitochondrial apoptotic cascade . -
pubmed
Profiling of
MAPKs revealed activation of
JNK upon
hesperetin treatment which was abrogated upon
NAC pre - treatment . -
pubmed
Additionally , inhibition of
JNK by
SP600125 significantly reversed
hesperetin - mediated apoptosis . -
pubmed
( + ) - Naloxone inhibits
morphine - induced chemotaxis via prevention of
heat shock protein 90 cleavage in microglia . -
pubmed
RESULTS : Our results showed that 1muM
morphine enhanced microglia activation and migration , decreased
alpha - tubulin acetylation , and induced
heat shock protein 90 (
HSP90 ) fragmentation and
histone deacetylase 6 (
HDAC6 ) expression . -
pubmed
Morphine - induced
alpha - tubulin deacetylation and
HSP90 fragmentation were
HDAC6 - dependent . -
pubmed
Pretreatment with
( + ) - naloxone ( 1nM ) inhibited
morphine - evoked microglia activation and chemotaxis and prevented
alpha - tubulin deacetylation and
HSP90 fragmentation by inhibiting
HDAC6 expression . -
pubmed
CONCLUSION : Based on the findings of the present study , we suggest that
( + ) - naloxone inhibits
morphine - induced microglia activation by regulating
HDAC6 - dependent
alpha - tubulin deacetylation and
HSP90 fragmentation . -
pubmed
A new type of bifunctional magnetic - fluorescent
Fe3O4@
SiO2 - PDI - PAA /
Ca ( 2 + ) nanoparticles has been prepared by coating
PDI - cored
star polymers (
PDI - PAA ) onto the surface of
Fe3O4@
SiO2 core - shell nanostructures . -
pubmed
RESULTS : The primary mechanism of action of
colchicine is
tubulin disruption . -
pubmed
difficile total counts and spores , cytotoxin titres , short chain
fatty acid concentrations , biometal concentrations ,
lactoferrin concentration and
iron content of
lactoferrin were monitored daily . -
pubmed
difficile spores occurred 6 days
post instillation of
clindamycin , followed by rapid vegetative cell proliferation and detectable toxin production . -
pubmed
difficile vegetative cell growth and toxin production , whereas
apo - bLf and
iron alone did not . -
pubmed
Immunostaining of the muscle and axon markers F59 ,
znp1 , and
zn5 revealed that
4 - MBC exposure leads to a disorganized pattern of slow muscle fibers and axon pathfinding errors during the innervation of both primary and secondary motor neurons . -
pubmed
Hes3 is a component of the
STAT3 -
Ser /
Hes3 Signaling Axis controlling the growth and survival of neural stem cells and other plastic cells . -
pubmed
Here we provide initial observations implicating
Hes3 in the
cuprizone model of
demyelination and remyelination . -
pubmed
Based upon this approach , we identify a
Janus kinase inhibitor and
dbcAMP as powerful inducers of
Hes3 gene expression . -
pubmed
Spectro - microscopic data at the
Fe L3 ,
La M5 , and
Al K X - ray absorption edges reveal differing levels of deposited
Fe on the ECAT catalysts corresponding with an overall loss in tetrahedral
Al within the
zeolite domains . -
pubmed
Two small - molecule inhibitors of
IGF-1R were chosen ,
NVP - ADW-742 (
IGF-1R - selective ) and
OSI-906 ( a dual
IGF-1R /
insulin receptor alpha [
IR - alpha ] inhibitor ) . -
pubmed
RESULTS : Novel mechanisms of resistance ( MOR ) emerged after dalotuzumab- ,
NVP -
ADW-742- , and
OSI-906 - based targeting of
IGF-1R. -
pubmed
MOR to dalotuzumab included upregulation of
IRS1 ,
PI3 K , and
STAT3 , as well as
p38 MAPK , which was also induced by
OSI-906 . -
pubmed
pEIF4E(
Ser209 ) , a key regulator of
Cap - dependent translation , was induced in
ridaforolimus - resistant ES cell lines . -
pubmed
Unusual
Horner ' s Syndrome in Recurrent
Breast Cancer : Evaluation Using
18F - FDG PET /
CT . -
pubmed
18F - fluorodeoxyglucose (
FDG ) positron emission tomography / computed tomography ( PET /
CT ) is a widely used imaging modality in the initial diagnosis of
cancer , treatment response evaluation and detection of
recurrence . -
pubmed
Clinicians suspected
Horner ' s syndrome , and the patient underwent
FDG PET /
CT for the evaluation of
recurrence that could cause
Horner ' s syndrome . -
pubmed
FDG PET /
CT demonstrated a focal hypermetabolic lesion in the right cervicothoracic junction area , corresponding to the preganglionic cervical sympathetic trunk . -
pubmed
In this case , we could detect the exact location of the recurring lesion that caused
Horner ' s syndrome using
FDG PET /
CT . -
pubmed
Methotrexate combined with
leflunomide exhibited a higher extension of
CAL ( p=0.005 ) , and anti -
TNF - alpha therapy with
methotrexate was associated with a lower extension of
CAL ( p=0.05 ) . -
pubmed
CRITICAL ISSUES : The molecular mechanism whereby
PXDN uses
HOBr as a reactive intermediate to cross - link
collagen IV , yet avoid collateral damage to nearby BM proteins , remains unclear . -
pubmed
As seen with the
sulfilimine cross - link of
collagen IV ,
hypohalous acid oxidative events may be beneficial in select situations rather than uniformly deleterious . -
pubmed
A large amount of recombinant
SEA (
rSEA ) was obtained by engineering technology and was purified by
Ni affinity chromatography column , and the expression and purity of
rSEA and
SEA were analyzed by
SDS - PAGE . -
pubmed
The result showed that large amount of
SEs expressed within a short time at 28 C or thereabouts , and there was no impurity bands in electrophorogram after
rSEA was purified by
Ni affinity chromatography column . -
pubmed
The result showed that the content of
formic acid in mobile phase was an important factor for ionization of SEs in ESI -
TOF . -
pubmed
[ SEs + nH+ + mNH4 + ] n+m+ was shown on ESI -
TOF spectra when
SEA was detected by ESI -
TOF in positive ion mode , and the numerical value of n+m was less than or equal to the number of basic
amino acids in SEs . -
pubmed
aeruginosa
G6PDH is also a key enzyme in the metabolism of various
carbon sources , such as
glucose ,
glycerol ,
fructose , and
mannitol . -
pubmed
This potentially enabled
Nec-1 to prevent
SA -
AKI . -
pubmed
A
C6 semi - laminectomy was performed in the control group , whereas a water - absorbent
polyurethane polymer was implanted into the
C6 epidural space in the compression group . -
pubmed
CD73 facilitates
tumor growth by upregulation of the
adenosine ( immunosuppressive factor ) in the
tumor microenvironment , however , its precise molecular mechanisms is not precisely understood . -
pubmed
We used chitosan
lactate (
ChLa ) nanoparticles ( NPs ) to deliver
CD73 - specific small interfering RNA ( siRNA ) into
cancer cells . -
pubmed
alpha - Adrenergic stimulation with
phenylephrine caused similar dose - dependent increases in resistance and pressure across salinities , indicating unaltered
alpha - adrenoceptor sensitivity . -
pubmed
Proteomic quantification identified the modulation of an important metabolic regulator ,
Fumarase , and concomitant accumulation of the metabolite
fumarate . -
pubmed
Two Coselected Distal Mutations in HIV-1
Reverse Transcriptase (
RT ) Alter Susceptibility to
Nonnucleoside RT Inhibitors and
Nucleoside Analogs . -
pubmed
In contrast , both mutations affected the ability of
RT to incorporate
nucleoside analogs . -
pubmed
A series of novel
BN tetraphene derivatives have been prepared successfully for the first time via a
post - functionalization strategy . -
pubmed
ANCR expression and intracellular
calcium ions were detected by quantitative real - time
polymerase chain reaction ( qRT - PCR ) and laser confocal microscopy , respectively . -
pubmed
Alkaline phosphatase (
ALP ) activity and
calcium nodules were examined by
ALP and
alizarin red staining assay , respectively . -
pubmed
Si - ANCR significantly increased the proliferation ,
ALP activity ,
calcium deposition of osteoblast cells and decreased apoptosis . -
pubmed
The
aglycon is generated by
megaenzyme polyketide synthases (
PKSs ) from diverse
acyl - CoA as precursor substrates . -
pubmed
In this review , we have discussed versatile approaches for generating a wide range of
macrolide structures by engineering the
PKS and
post -
PKS cascades at either enzyme or cellular level in actinobacteria species , either the native or heterologous producer
strains . -
pubmed
Here we use
hydrogen /
deuterium exchange mass spectrometry , nuclear magnetic resonance ( NMR ) , and evolutionary sequence comparisons to map where and how the PHTH domain affects the
Bruton ' s tyrosine kinase (
BTK ) domain . -
pubmed
Through statistical comparisons of diverse
tyrosine kinase sequences , we identify residues unique to
BTK that coincide with the experimentally determined PHTH - binding surface on the
kinase domain . -
pubmed
All of the data suggested
CDE possessed potential anti - osteoporotic activity and this effect was , at least in part , involved in modulation of
RANKL /
RANK /
TRAF6 - mediated
NF - kappaB and
PI3K /
AKT signaling as well as
c - Fos and
NFAT2 levels . -
pubmed
The optimal extractions of
TPC and AA were explored by varying the key variables , including the extraction temperature ,
ethanol concentration , extraction time , and ultrasonic power , through statistical optimization . -
pubmed
In the variation experiment , the maximum
TPC and AA values of 9.8 mg GAE / g and 91.8 % were obtained respectively with an extraction temperature of 74.4 C ,
ethanol concentration of 55.4 % , extraction time of 59.6 min , and ultrasonic power of 700 W. HPLC coupled with diode array detection was used to identify and quantify the
phenolic compounds in the extracts , and
EGCG ( 0.12 mg / g DM ) was identified as a major peak in the analysis , demonstrating that high value - added material with a bioactive property can be produced from LEM . -
pubmed
LEM has a high
TPC value , particularly with regard to
EGCG , and excellent AA , considering it is highly used as a functional material for food , cosmetics , and medicine . -
pubmed
There was increased
RPE - related fb -
P3 amplitude for those in the high
alcohol use group . -
pubmed
We found that high
alcohol use participants had attenuated FRN amplitude in contrast with low
alcohol use participants for both positive and negative RPEs but enhanced fb -
P3 for both positive and negative
RPE . -
pubmed
To clarify the strength and underlying mechanism of
amino acid binding to
pyrite surfaces , we measured the unbinding ( pull - off ) force of ten
amino acids and two oligo - peptides from water -
pyrite interfaces using atomic force microscopy (
AFM ) . -
pubmed
had negative correlations with
lysophospholipids metabolites , and three of them had also negative correlations with serum
IL-17alpha . -
pubmed
We review two phase III trials of
rituximab , a chimeric
CD20 monoclonal antibody , used for the treatment of
pemphigus vulgaris (
PV ) . -
pubmed
This behavior was relatively accorded with the average oxidation state (
AOS ) results , evidencing the potential of EF process in enhancing the biodegradability of
CFPZ . -
pubmed
Analysis of 37 - S RNA , the common precursor to 17 - S , 5.8 - S and 26 -
S rRNA and most probably the primary ribosomal transcript , shows that this RNA molecule contains already most if not all of the 36 - 37
pseudouridine residues found in the mature rRNAs . -
pubmed
The
pseudouridine residues within the rRNA sequences are apparently clustered to a certain degree as can inferred from the occurrence of a single oligonucleotide containing 3
pseudouridines , which was obtained by digestion of 26 -
S rRNA with
ribonuclease T1 . -
pubmed
Porcine pancreatic elastase (
EC 3.4.21.11 ) has been immobilized on
polyacrylamide beads using
glutaraldehyde ad bridging reagent without important loss of catalytic activity . -
pubmed
A
nitroxide spin label ,
1 - oxyl-2,2,5,5 - tetramethyl-4 - piperidinyl - ethylphosphonofluoridate , reacting covalently with the
serine-195 residue of the active centre of free
elastase was used as a conformational and dynamical electron spin resonance probe . -
pubmed
The
charcoal -
coil : a dialysis - dialysate regeneration system . -
pubmed
Fatty acids of medium chain length (
C6 to CII ) at 0 - 4 % produced a decrease in cell concentration ,
nonanoic and decanoic acids being the most effective . -
pubmed
The gross morphology was revealed by
NADH - diaphorase histochemistry , and the shape of the neurons by
silver impregnation . -
pubmed
Three morphologically distinct cell types were revealed by
silver impregnation : mainly type I and type
II neurons cells were seen in the duodenum , while the
rest of the intestine contained type
II and III cells . -
pubmed
Gamma - aminobutyric acid showed non - uniform distribution in the two parts of the midgut : a stronger and more regular fibre staining was found in the duodenum then in the
rest of the intestine . -
pubmed
Following treatment of ROS cells with
Pb2 + , intracellular levels of the
calcium - binding protein
osteonectin /
SPARC were increased . -
pubmed
These studies suggest that processes associated with
osteonectin /
SPARC translation and secretion are sensitive to
Pb2 + . -
pubmed
266 , 16633 - 16635 ) and the increased
water permeability in oocytes that express mRNA encoding a 28 - kDa erythrocyte protein (
CHIP28 , Preston , B. M. , Carroll , T. P. , Guggino , W. B. , and Agre , P. ( 1992 ) Science 256 , 385 - 387 ) . -
pubmed
Pf in proteoliposomes reconstituted with solubilized
CHIP28 was high ( Pf = 0.03 cm / s , 37 degrees C ) , the activation energy was low ( 2.2 kcal / mol ) , and Pf was decreased by greater than 50 - fold by mercurial
sulfhydryl reagents and
Me2SO . -
pubmed
The positive inotropic and chronotropic actions of
parathyroid hormone (
PTH ) in cardiac cells are considered to be related to the modulation of
calcium influx . -
pubmed
The active synthetic fragment of
bovine PTH ,
bPTH -( 1 - 34 ) , at a concentration of 1 microM , significantly enhanced the magnitude of L - channel currents by 67.8 % ( n = 13 , p less than 0.01 ) . -
pubmed
The suppression or amplification of
PTH - induced enhancement of inward currents by
nifedipine , 1 microM , or Bay
K-8644 , 5 microM , respectively , further indicated that the effect of
PTH was specific for
L - type calcium channels . -
pubmed
However ,
bPTH -( 1 - 34 ) failed to modulate the magnitude or kinetics of
T - channel currents . -
pubmed
Ion replacement experiments and sensitivity to
furosemide or a
Cl - channel blocker indicated that
PLC induced
Cl- secretion . -
pubmed
The effect of
PLC was blocked by the
protein kinase inhibitor ,
staurosporine . -
pubmed
Carbachol , when added during the second phase of the
PLC response , induced a ' paradox ' change in Isc : a rapid , transient increase in Isc was followed by a long - lasting decrease . -
pubmed
This inhibition of the
PLC response was more pronounced after elevation of the external
Ca2 + concentration . -
pubmed
A
Ca2 + ionophore ,
ionomycin , and a
Ca2 + channel activator ,
BAY K 8644 , also inhibited the
PLC response . -
pubmed
The results suggest dual dependence of the action of
PLC on the intracellular
Ca2 + concentration . -
pubmed
In the plantaris ,
triglyceride content was unchanged , but
citrate synthase activity in H ( 210 + /- 13 mumol min-1 g-1 ) was significantly greater than in
SA ( 177 + /- 10 ) . -
pubmed
According to those results , the superior sagittal
sinus was supposed to have the incomplete occlusion or delayed blood flow that were not observed by DSA , MRI and
CT scan performed previously . -
pubmed
Those occlusive change in the superior sagittal
sinus impeded the
CSF absorption and elevated the pressure of venous inflow , then the arterio - venous communication has been developed. -
pubmed
The following parameters were determined : ( 1 ) involution and damage of the endothelial cells by scanning and transmission electron microscopy ; ( 2 ) incidence and weight of
thrombi in the lumens of the veins after 2 h stasis , and ( 3 ) effects of
heparin on APTT and
anti - Xa activity . -
pubmed
Previous investigations have shown that the catalytic efficiency ( kcat / KM ) of
pyruvate oxidase can be enhanced 450 - fold by chymotryptic cleavage of a 23 - residue peptide ( alpha - peptide ) from the
carboxy terminus of the enzyme . -
pubmed
The extent of
carboxypeptidase hydrolysis was followed by analyzing the release of
amino acids and by mass spectral analysis of the truncated alpha - peptides which were derived from the
carboxypeptidase - treated preparations . -
pubmed
The results indicate that the removal of 7
carboxy - terminal residues does not activate the enzyme whereas the removal of 10 or 11 residues produces activated
pyruvate oxidase . -
pubmed
Endoproteinase Glu - C specificity predicts hydrolytic cleavage of the peptide bond between
Glu-561 and
Val-562 with the removal of 11 residues from the
carboxy terminus of the enzyme . -
pubmed
Reaction of certain
flavoproteins , the
NADH : oxidoreductases , with different
quinones is well known to form
hydrogen - peroxide . -
pubmed
Different redox cycling compounds are shown to be active in this "
diaphorase " reaction by lens protein extract (
LCE ):
Oxygen consumption can be detected in the presence of
pyrroloquinoline quinone and
juglone whereas
1,4 - naphthoquinone ,
menadione and
paraquat are no redox cyclists in this
flavoprotein catalyzed reaction . -
pubmed
This excitation was associated with blockade of IPSPs , represented by positive waves , at a presynaptic site , which was antagonised by
PDAc suggesting the involvement of
PKC . -
pubmed
4 )
Sphingosine , a dual
PKC / calcium - calmodulin - dependent kinase inhibitor , did not reproduce H-7 - induced responses . -
pubmed
In conclusion , in the hippocampus ,
PKC can modulate the IPSPs , the anomalous rectification , and the membrane potential , but
PKC is unlikely to be the major intracellular mediator of the excitatory actions of
acetylcholine . -
pubmed
Nucleotides are known to enhance
cyclooxygenase product formation in several tissues and , in addition , are believed to function as cofactors for mammalian
5 - lipoxygenases . -
pubmed
We found that the
phosphorylated adenine derivatives (
ATP ,
ADP , and
AMP ) increased the formation of
12 - hydroxyeicosatetraenoic acid (
12 - HETE ) by 2 - fold without altering the formation of
cyclooxygenase products . -
pubmed
This suggests that the
12 - lipoxygenase enzyme of platelets can be regulated by
adenine nucleotides . -
pubmed
We next determined the portion of the
nucleotide molecule responsible for the enhanced
12 - lipoxygenase activity of platelets . -
pubmed
In conclusion , platelet
12 - lipoxygenase was stimulated by
ATP , as is true for several mammalian
5 - lipoxygenases . -
pubmed
However ,
cyclooxygenase product formation by platelets was not altered by
nucleotide addition . -
pubmed
These studies suggest that following in vivo
injury or
platelet aggregation , when local concentrations of
nucleotides are high ,
platelet lipoxygenase activity may be stimulated . -
pubmed
A single topical treatment with
12 - O - tetradecanoylphorbol-13 - acetate caused a rapid initial decrease in the epidermal transcript levels corresponding to the Mr 67,000 and 59,000
keratin subunits . -
pubmed
In contrast , the transcript level for the Mr 55,000 subunit was increased substantially 12- 48 h after treatment , the Mr 50,000 subunit transcript increased to a lesser extent , and the Mr 60,000 subunit
message was transiently decreased at 12 h but returned to the level of solvent - treated skin by 24 h. Single exposure to the incomplete
tumor promoters
4 - O - methyl-12 - O - tetradecanoylphorbol-13 - acetate , the ionophore
A23187 , and
mezerein induced changes in
keratin gene transcripts similar to those of
12 - O - tetradecanoylphorbol-13 - acetate . -
pubmed
Six
NHS , acting in
CHF , have presently been identified : three of them induce vasoconstriction and
sodium retention ( sympathetic nervous systems ,
renin -
angiotensin -
aldosterone system and
arginine - vasopressine system ) ; the remaining three offset or balance the former ones , acting , therefore as " counterregulators " (
prostaglandins --
PGE2 and
PGI2 - - , dopaminergic system and
atrial natriuretic factor ) . -
pubmed
The onset and offset of the plasma
melatonin rhythm in
DD after 3 weeks of the respective light treatments was assessed for 48 hr , immediately after transferring to DD . -
pubmed
The
cytochrome oxidase activities ( measured with
ascorbate +
phenazine methosulfate as substrates ) were also decreased , but this segment of the respiratory chain was not rate - limiting for
succinate or
NAD - linked substrate oxidation . -
pubmed
Analyses of the steady - state reduction kinetics of the respiratory chain carriers revealed that the rate - limiting step of the impaired respiration with
succinate or
NAD - linked substrates lies between the
c cytochromes and
cytochrome oxidase . -
pubmed
Glucose counterregulation ,
hypoglycemia , and intensive
insulin therapy in
diabetes mellitus . -
pubmed
The enhanced presentation of
ferritin conjugated to
goat anti - IgG ( H + L ) or to
anti - IgM was sensitive to radiation doses greater than 2000 R , and was effective at less than one - tenth the number of spleen cells , consistent with the predominance of B cells as antigen - presenting cells for this form of the
antigen rather than macrophages and dendritic cells only . -
pubmed
Effect of
magnesium on production of
toxic - shock - syndrome toxin-1 : a collaborative study . -
pubmed
We performed a series of collaborative experiments to clarify the effect of
Mg ++ on production of
toxic - shock - syndrome toxin-1 (
TSST-1 ) in various culture media . -
pubmed
[ Evaluation of photocoagulation of the posterior
pole with
argon laser in edematous
diabetic retinopathy ] . -
pubmed
Use of
macrophage inhibition factor and mast - cell degranulation tests for diagnosis of
cloxacillin - induced
cholestasis . -
pubmed
Cloxacillin - induced
cholestasis was diagnosed with the help of the
macrophage inhibition factor and mast - cell degranulation tests . -
pubmed
We conclude that the abnormalities of the divalent cation and
phosphate metabolism can not be accounted for in full by increased circulating
PTH activity , and are predominantly due to an intrinsic
renal abnormality . -
pubmed
The sleep promoting effects of
Factor S can not be duplicated by
serotonin ,
4 - OH - butyrate ,
butyrolactone ,
GABA (
gamma - amino butyric acid ) ,
glutamic acid or
3 ' , 5 '- cyclic AMP when these substances are added to control fluids in concentrations up to 10 times greater than those found in normal c.s.f.5 . -
pubmed
Hemoglobin Kariya [
alpha 40 ( C5 ) Lys leads to
Glu ] : a new
hemoglobin variant with an increased
oxygen affinity . -
pubmed
This
hemoglobin showed an increased
oxygen affinity , decreased
heme -
heme interaction and a lowered
2,3 - DPG effect . -
pubmed
An
IgM lambda monoclonal human antibody recognizing a
sialic acid determined antigen fully expressed on newborn erythrocytes . -
pubmed
Papainization of erythrocytes enhanced markedly the susceptibility of the corresponding antigen not only to
antibody binding but also to the action of
neuraminidase , indicating that
sialic acid is the immunodominant component of the cryptic antigen . -
pubmed
Insulin - like partial effects of
agmatine derivatives in adipocytes . -
pubmed
In previous investigations , we described
insulin - like effects of
agmatine [
( 4 - aminobutyl ) guanidine ] in vitro . -
pubmed
In the present work we have examined whether these effects of
agmatine can be enhanced by variation in chain length ( C3 and
C5 forms ) and by alkylation . -
pubmed
In our test system ,
insulin decreases
adrenalin - induced lipolysis to 40 % of the control value ( 100 % ) . -
pubmed
Some of the
glycoprotein /
sialic acid of this internal membrane system ( 30 % ) remains bound for a considerable length of time . -
pubmed
( 5 ) 3H - NAm was a more specific marker of
glycoprotein than
2H - NAG . -
pubmed
The following radioactive precursors , either combined or separately , dissolved in 50 microliter of saline containing 15 %
BSA , were injected into vitreous body :
[ 2 - 3H ] glycerol ( 50 microCi ) ,
[ 1 - 14C ] palmitate ( 15 microCi ) , and
[ 1 - 14C ] linoleate ( 7.5 microCi ) . -
pubmed
In order to obtain detailed information about the efferent connections of these lateral cortex subdivisions , iontophoretic injections of the anterograde tracers
Phaseolus vulgaris leucoagglutinin and
biotinylated dextran were made in the various parts . -
pubmed
Regulation of volume activated
chloride channels by
protein kinase C - mediated phosphorylation of
P - glycoprotein . -
pubmed
The multidrug resistance
P - glycoprotein (
Pgp ) transports hydrophobic drugs out of cells and has been recently associated with volume - activated
chloride channels . -
pubmed
Replacement of these residues with
glutamate , to mimic the effect of phosphorylation , mimicked the effects of
PKC on channel activation . -
pubmed
These results indicate that overexpression of
Pgp confers
PKC - regulation of endogenous volume - activated
chloride channels . -
pubmed
More generally they favour a model in which
Pgp acts as a regulator of volume - activated
chloride channels . -
pubmed
Four treatment regimens were compared : radiation treatment alone ( I ) , combined radiation , drug and
estrogen treatments ( II ) ,
estrogen therapy alone ( III ) , combined
estrogens and radiation (
IV ) . -
pubmed
NIR gene encodes a protein of 378
amino acid residues including a putative signal peptide of 37 residues . -
pubmed
The DNA - derived
amino acid sequence of
NIR is in complete agreement with that from Edman degradation and the DNA coding sequence of
BCP is also consistent with its partial N - terminal
amino acid sequence . -
pubmed
The correlation between the concentrations of
myoglobin and
malonic dialdehyde suggests that there is an association between
hypermyoglobinemia and the status of cellular membranes . -
pubmed
Propeptide and
glutamate - containing substrates bound to the
vitamin K - dependent carboxylase convert its
vitamin K epoxidase function from an inactive to an active state . -
pubmed
The
vitamin K - dependent gamma - glutamyl carboxylase catalyzes the posttranslational conversion of
glutamic acid to
gamma - carboxyglutamic acid in precursor proteins containing the gamma - carboxylation recognition site ( gamma - CRS ) . -
pubmed
We show that in the absence of
gamma - CRS - containing propeptide and/or
glutamate - containing substrate ,
carboxylase has little or no
epoxidase activity . -
pubmed
Epoxidase activity is induced by
Phe - Leu - Glu - Glu - Leu ( FLEEL ) ( 9.2 pmol per min per pmol of
enzyme ) , propeptide , residues -18 to -1 of
proFactor IX ( 3.4 pmol per min per pmol of
enzyme ) ,
FLEEL and propeptide ( 100 pmol per min per pmol of
enzyme ) , and proPT28 ( HVFLAPQQARSLLQRVRRANTFLEEVRK , residues -18 to +10 of
human acarboxy - proprothrombin ) , ( 5.3 pmol per min per pmol of
enzyme ) . -
pubmed
These results indicate that in the absence of propeptide or
glutamate - containing substrate , oxygenation of
vitamin K by the
carboxylase does not occur . -
pubmed
Upon addition of propeptide or
glutamate - containing substrate , the
enzyme is converted to an active
epoxidase . -
pubmed
X - ray diffraction data analysis shows that noncrystallographic symmetry - related
arcelin-1 molecules form a
lectin - like dimer and reveals the presence of a solvent - exposed anion binding site on the protein , at a crystal - packing interface . -
pubmed
Transferrin conjugates of
doxorubicin : synthesis , characterization , cellular uptake , and in vitro efficacy . -
pubmed
Thus , thiolated
human serum transferrin was conjugated with four
maleimide derivatives of
doxorubicin that differed in the stability of the chemical link between drug and spacer . -
pubmed
Resonance raman characterization of the
heme domain of soluble
guanylate cyclase . -
pubmed
We propose that the absence of
hydrogen - bond formation between a distal residue and exogenous ligands is physiologically relevant in lowering the
oxygen affinity of heterodimeric
sGC and , therefore , stabilizing the
ferrous , active form of the
enzyme under aerobic conditions . -
pubmed
High affinities of Ki = 0.81 and 2.6 nM for the
[ 3H ]- cytisine rat brain nicotinic acetylcholine binding sites were found for the
5 - pyrimidinyl and the
5 -( 2 - amino )- pyrimidinyl epibatidine analogues , respectively . -
pubmed
Superstructures and defect structures revealed by atomic - scale
STM imaging of
WO3(001 ) . -
pubmed
Stretch - induced increases in growth were completely blocked on addition of anti -
IGF - I and partially blocked with
platelet - derived growth factor (
PDGF ) antibodies and with a
tyrosine kinase inhibitor (
tyrphostin-1 ) . -
pubmed
In the present study , we investigated whether
serine phosphorylation of the
SR Ca ( 2 +)- ATPase occurs in the intact functioning heart . -
pubmed
Following 45 - 50 min of
( 32 ) P perfusion , the hearts were freeze - clamped ,
SR isolated , and analyzed for protein phosphorylation . -
pubmed
SDS - polyacrylamide gel electrophoresis and autoradiography showed phosphorylation of several peptides including the
Ca ( 2 +)- ATPase and
Ca ( 2 + ) release channel (
ryanodine receptor ) . -
pubmed
The
Ca ( 2 +)- ATPase showed immunoreactivity with a
phosphoserine monoclonal antibody indicating that the in situ phosphorylation occurred at the
serine residue . -
pubmed
Quantification of
Ca ( 2 +)- ATPase phosphorylation in situ yielded a value of 208 + /- 12 pmol ( 32)P / mg
SR protein which corresponded to the phosphorylation of approximately 20 % of the
Ca ( 2 + ) pump units in the
SR membrane . -
pubmed
, in the absence of any inotropic intervention ) , a considerable steady - state pool of
serine - phosphorylated
Ca ( 2 +)- ATPase likely exists in the normally beating heart . -
pubmed
These findings demonstrate that
serine phosphorylation of the
Ca ( 2 +)- ATPase is a physiological event which may be important in the regulation of
SR function . -
pubmed
At 6 to 24 h after
H ( 2 ) O ( 2 ) injection , the level of
LPO was significantly higher in the
H ( 2 ) O ( 2 ) group than in the
H ( 2 ) O ( 2 ) + pHGF - treated group . -
pubmed
This finding indicated that
pHGF protected against cell membrane damage in
H2O2 - induced
acute lung injury . -
pubmed
In the
H ( 2 ) O ( 2 ) +
pHGF - treated group , TUNEL - positive signals were reduced compared with those in the
H ( 2 ) O ( 2 ) group . -
pubmed
In the
H ( 2 ) O ( 2 ) +
pHGF - treated group , the formation of subendothelial
blebs was seen , but no severe subendothelial
blebs were observed . -
pubmed
These findings indicated that
pHGF protects the progression of
H ( 2 ) O ( 2 )-induced
acute lung injury , and showed that
pHGF acts to stabilize the cell membrane in capillary endothelial cells and lung epithelial cells . -
pubmed
A series of
cis-1 ( S ) 2 ( R )- amino-2 - indanol based compounds with a
biphenylmethyl group at the
P1 ' position was found to be potent
aggrecanase inhibitors . -
pubmed
Inhibition of
estrone sulfatase (
ES ) by derivatives of
4 -[( aminosulfonyl ) oxy ] benzoic acid . -
pubmed
In our search for potent inhibitors of the
enzyme estrone sulfatase (
ES ) , we have undertaken the synthesis and biochemical evaluation of a range of straight chain
alkyl esters of
4 -[( aminosulfonyl ) oxy ] benzoic acid . -
pubmed
Gadodiamide and
gadopentetate dimeglumine in MRI versus spiral
CT in the diagnosis of
liver lesions . -
pubmed
In addition , the ratio of activated
tyrosine phosphorylated
STAT3 to total
STAT3 was not significantly altered in failing hearts compared with non - failing controls . -
pubmed
CpZPC , a newt
ZPC molecule , localizes to the inner surface of the egg envelope . -
pubmed
Two bands corresponding to 84 kDa and 70 kDa in the egg envelopes were detected by immunoblotting with an antiserum raised against a 9
amino acid peptide in the C - terminus domain of
cpZPC . -
pubmed
In all species studied to date , the function of
heat shock protein 90 (
Hsp90 ) , a ubiquitous and evolutionarily conserved molecular chaperone , is inhibited selectively by the natural product drugs
geldanamycin (
GA ) and
radicicol . -
pubmed
Mechanism of inhibition of
Catalase by
3 - amino-1,2,4 - triazole . -
pubmed
Both
( 1 ) H- and
( 31 ) P -
MRS have revealed significant disturbances in
amino acids , lipids , and
phosphorus - containing metabolites within
tumors . -
pubmed
( 1 ) H -
MRS has been successfully used to quantify the extent of neuronal cell loss imposed on the brain during radiotherapy . -
pubmed
( 19 ) F -
MRS opens access to artificially induced
fluorocompounds such as
5 - fluorouracil and its metabolites . -
pubmed
Acute renal failure was not observed in this dosage of oral
nickel administration by analysing
NAG ,
beta2 - microglobulin ,
urine albumin and urine protein . -
pubmed
METHODS :
Ischemia - reperfusion injury was studied in forearm skeletal muscle by
technetium Tc 99m - labeled
annexin A5 scintigraphy . -
pubmed
Immediately on reperfusion ,
annexin A5 labeled with
technetium Tc 99 m was administered intravenously , and
ischemia -
reperfusion injury was expressed as the percentage difference in radioactivity between the experimental arm and the control arm 1 and 4 hours after reperfusion . -
pubmed
RESULTS :
Dipyridamole treatment significantly reduced
annexin A5 targeting in skeletal muscle compared with the control group ( thenar region , 13 % + /- 7 % versus 22 % + /- 15 % at 1 hour after reperfusion and 9 % + /- 6 % versus 27 % + /- 13 % at 4 hours for
dipyridamole and control groups , respectively [ P = .01 ] ; flexor region , 4 % + /- 8 % versus 7 % + /- 6 % at 1 hour after reperfusion and 1 % + /- 4 % versus 10 % + /- 9 % at 4 hours for
dipyridamole and control groups , respectively [ P = .01 ] ) . -
pubmed
OBJECTIVES : Adding
ribavirin to
interferon improves treatment response for patients with
chronic hepatitis C , but the effects of
ribavirin monotherapy are unclear . -
pubmed
Ribavirin had no significant effect on sustained ( risk difference (
RD ) , 0 % ; 95 % confidence intervals ( CI ) , -2 % to 3 % ) or end - of - treatment virological response (
RD , 0 % ; 95 % CI , -3 % to 3 % ) . -
pubmed
Ribavirin had no significant effect on liver - related morbidity plus mortality (
RD , 0 % ; 95 % CI , -2 % to 3 % ) . -
pubmed
Purified
gamma - sm was used as
antigen to screen the displayed phage hybrid antibody library rescued by helper phage M13K07 for three rounds . -
pubmed
PURPOSE :
Autosomal dominant Stargardt - like ( STGD3 ) disease results from mutations in the
ELOVL4 gene ( elongation of
very - long - chain fatty acids ) . -
pubmed
Characterization included examining gene
message and protein levels , electroretinogram (
ERG ) , retinal morphology and ultrastructure , and plasma and retinal
fatty acid composition . -
pubmed
In order to determine whether increase in global protein glutathionylation by
HEDS leads to loss of function of an important protein , we compared the effect of
HEDS on global protein glutathionylation with that of
Ku protein function , a multifunctional
DNA repair protein , using a novel ELISA . -
pubmed
E89 cells treated with
HEDS showed a significant loss of
Ku protein binding to DNA . -
pubmed
TRPM5 is known to be abundantly expressed in taste receptor cells where it participates in sweet ,
amino acid and bitter perception . -
pubmed
In addition , a time course experiment was set up to determine how the transcription profile of
TbICL ,
TbMLS and
TbFBP in axenic - grown mycelia is affected by different
carbon sources . -
pubmed
The high induction of
TbICL ,
TbMLS and
TbFBP transcription and the translocation of lipids in fruiting bodies let us hypothesize that
glyoxylate cycle and gluconeogenesis are key metabolic pathways in the recycling of existing cell material and the channelling towards the biosynthesis of new cell components during the maturation of fruiting bodies . -
pubmed
Clavulanic acid and
cephamycin C production are improved in the
DeltaareB mutant although no drastic difference in
ccaR expression was observed . -
pubmed
The
azoles are relatively safe , but clinicians should be aware of drug - drug interactions and adverse effects , including
visual disturbances ( with
voriconazole ) , elevations in
liver transaminase levels , and
skin rashes . -
pubmed
There have been few detailed studies of binding characterizations for the complex formation by
TNFalpha and clinically relevant antagonists , particularly Infliximab (
Remicade ) and Etanercept (
Enbrel ) . -
pubmed
Bacterial and yeast two - hybrid experiments revealed a complex network of interactions involving
TagA , - B , - E , - F , - G , - H , and - O and the cell shape determinants
MreC and
MreD ( encoded by the
mreBCD operon and presumably involved in the spatial organization of
PG synthesis ) . -
pubmed
The treatment plan for this patient included the following medications :
pioglitazone hydrochloride ( a
thiazolidinedione , 30 mg / d ) ;
irbesartan ( an
angiotensin receptor blocker , 150 mg / d titrated to 300 mg / d ) ;
hydrochlorothiazide ( an antikaliuretic agent , 12.5 mg / d ) ; and
aspirin ( 325 mg / d ) . -
pubmed
Sitagliptin phosphate ( a
dipeptidyl peptidase IV inhibitor , 50 mg / d ) was added to the treatment regimen to improve glycemic control . -
pubmed
Preliminary study of
fibrin glue combined with
pingyangmycin for the treatment of
venous malformations in the oral and maxillofacial region . -
pubmed
PURPOSE : To observe the outcome of using
fibrin glue ( FG ) combined with
Pingyangmycin ( Tianjin Taihe Pharmaceutical Co Ltd , Tianjin , China ) in the treatment of
venous malformations (
VMs ) in the oral and maxillofacial region . -
pubmed
In all of these cells , K channel activity , reflected in the net , steady - state outward
K + currents (
IK ) , was inversely related to the plasma membrane
PtdInsP2 level . -
pubmed
Consistent with this , short - term manipulations decreasing
PtdInsP2 levels in the High PIs , such as pretreatment with the phytohormone
abscisic acid ( 25 microM ) or neutralizing the bath solution from pH 5.6 to pH 7 , increased
IK ( i.e. -
pubmed
These results are consistent with
NtORK inhibition by the negatively charged
PtdInsP2 in the internal plasma membrane leaflet . -
pubmed
Polymerase chain reaction of a
urate anion exchanger known to regulate blood
urate level (
SLC22A12 gene :
URAT1 ) demonstrated that homozygous mutations in exon 4 ( W258X ) . -
pubmed
Two hundred and forty fish ( average weight : 27.01 g / fish ) , distributed equally into 4 different groups ( control , T(1 ) , T(2 ) , and T(3 ) ) , each with 6 replicates were fed with control ( 40 % crude protein ,
CP and 0.1 %
vitamin C ) , T(1 ) ( 40 %
CP and 0.1 %
vitamin C ) ,
T ( 2 ) ( 50 %
CP and 0.1 %
vitamin C ) , and
T ( 3 ) ( 50 %
CP and 0.2 %
vitamin C ) diets for 90 days . -
pubmed
Results indicated significant reduction in the growth performance , survival , and activities of
lactate dehydrogenase ( liver and muscle ) ,
malate dehydrogenase ( liver and muscle ) , enzymes of protein metabolism (
aspartate amino transferase in liver and
alanine amino transferase in liver and muscle ) ,
acetyl choline esterase ( brain ) ,
alkaline phosphatase activity ( liver ) , and
ATPase ( gill ) enzymes of group fed control diet and exposed to
endosulfan . -
pubmed
Further , high
CP and
vitamin C diet reduced
endosulfan accumulation in the muscle . -
pubmed
Each side was randomly assigned to the control group ( CG : bioabsorbable membrane made of
glycolide and
lactide copolymer ) or the test group (
TG :
ADM as a membrane ) . -
pubmed
These include
nucleoside and
nonnucleoside reverse transcriptase inhibitors ,
protease and integrase strand transfer inhibitors ,
CCR5 antagonists , and an entirely new class of maturation inhibitors . -
pubmed
Neutralization of
IL-17 suppressed the
hepatotoxic effect of
halothane . -
pubmed
In conclusion , we demonstrated that
IL-17 is involved in the
halothane - induced
liver injury . -
pubmed
The maximum burning velocity of 1234yf was found to be 1.2+/-0.3 cm s(-1 ) , which was approximately one - fifth that of
HFC-32 ( 6.7 cm s(-1 ) ) and one order of magnitude less than those of 1243yf ( 19.8 cm s(-1 ) ) and 1243zf (
14.1 cm s(-1 ) ) . -
pubmed
Prediction of
asthma in symptomatic preschool children using exhaled
nitric oxide , Rint and specific
IgE. -
pubmed
Associations of FE(
NO ) ( n=308 ) , Rint ( n=482 ) and specific
IgE ( n=380 ) at 4 years with
wheezing and
asthma at the ages of 5 - 8 years were assessed using repeated measurement analyses . -
pubmed
RESULTS : FE(
NO ) and specific
IgE measured at 4 years were associated with
wheezing and
asthma at 8 years . -
pubmed
Both tests also remained significant predictors after mutual adjustment and adjustment for clinical history : OR on
wheezing at 8 years for FE(
NO ) ( ( 10)log - scale , per IQR ) 1.6 ( 95 % CI 1.1 to 2.2 ) and for specific
IgE 2.8 ( 95 % CI 1.9 to 4.1 ) . -
pubmed
CONCLUSIONS : In preschool children with symptoms suggestive of
asthma , both FE(
NO ) and specific
IgE measured at age 4 , but not Rint , improved the prediction of
asthma symptoms until the age of 8 years , independent of clinical history . -
pubmed
Expression profiles of genes in
CRPC support a role for the transcriptional activity of the
androgen receptor (
CCNH ,
CUEDC2 ,
FLNA ,
PSMA7 ) ,
steroid synthesis and metabolism (
DHCR24 ,
DHRS7 ,
ELOVL5 ,
HSD17B4 ,
OPRK1 ) , neuroendocrine (
ENO2 ,
MAOA ,
OPRK1 ,
S100A10 ,
TRPM8 ) , and proliferation (
GAS5 ,
GNB2L1 ,
MT - ND3 ,
NKX3 - 1 ,
PCGEM1 ,
PTGFR ,
STEAP1 ,
TMEM30A ) , but neither supported nor discounted a role for cell survival genes . -
pubmed
Characterization of
nicotinamidases : steady state kinetic parameters , classwide inhibition by
nicotinaldehydes , and catalytic mechanism . -
pubmed
Nicotinamidases are metabolic enzymes that hydrolyze
nicotinamide to
nicotinic acid . -
pubmed
Nicotinaldehyde was determined to be a potent competitive inhibitor of these
enzymes , binding in the low micromolar to low nanomolar range for all
nicotinamidases tested . -
pubmed
Inhibitions are consistent with reaction of the universally conserved catalytic
Cys on each
enzyme with the
aldehyde carbonyl carbon to form a
thiohemiacetal complex that is stabilized by a conserved
oxyanion hole . -
pubmed
pneumoniae
nicotinamidase can catalyze exchange of
( 18 ) O into the
carboxy oxygens of
nicotinic acid with
H ( 2 )( 18 ) O. -
pubmed
Disease activity , lipid profile , serum
malondialdehyde (
MDA ) ,
tumor necrosis factor - alpha (
TNF - alpha ) ,
resistin ,
adiponectin , and brachial artery flow - mediated dilation (
FMD ) were measured before and after 6 months of treatment . -
pubmed
RESULTS :
Atorvastatin combined with
MTX therapy significantly reduced serum total
cholesterol ,
low - density lipoprotein cholesterol , and
triglycerides , and increased
high - density lipoprotein cholesterol ( p & lt ; 0.001 ) . -
pubmed
Disease activity variables , serum
MDA ,
TNF - alpha ,
resistin ,
adiponectin , and
FMD were significantly improved by the drug combinations ( p & lt ; 0.001 ) . -
pubmed
In the present study , we determined whether a
cyclophosphamide - induced reduction of naturally occurring regulatory T cells ( Tregs ) was associated with the anti -
tumor activity in our nonmyeloablative
SCT system . -
pubmed
These results indicated that
cyclophosphamide - induced reduction of recipient Tregs is associated with retardation of
tumor progression via the expansion of host - reactive donor T cells and
IFN - gamma production after DLI in our nonmyeloablative
SCT system . -
pubmed
Inhibition of the
MDM2 -
p53 interaction using either
Nutlin-3 or
MDM2 RNA interference resulted in hyperactivation of the
p53 pathway and a strong induction of apoptosis in
cisplatin - sensitive and -resistant TC cells . -
pubmed
Suppression of
wild - type p53 induced resistance to
Nutlin-3 in
TC cells , demonstrating the key role of
p53 for
Nutlin-3 sensitivity . -
pubmed
More specifically , our results indicate that
p53 - dependent induction of
Fas membrane expression ( ~threefold ) and enhanced
Fas /
FasL interactions at the cell surface are important mechanisms of
Nutlin-3 - induced apoptosis in
TC cells . -
pubmed
Importantly , an analogous
Fas - dependent mechanism of apoptosis upon
Nutlin-3 treatment is executed in wild - type
p53 expressing
Hodgkin lymphoma and
acute myeloid leukaemia cell lines . -
pubmed
Finally , we demonstrate that
Nutlin-3 strongly augmented
cisplatin - induced apoptosis and cell kill via the
Fas death receptor pathway . -
pubmed
ROS was determined using the reactive dye ,
Dichlorodihydrofluorescin (
DCFH ) , and the Plasmid Scission Assay (
PSA ) , which determine DNA damage . -
pubmed
These " pleiotropic " effects stem from their inhibition of prenylation of the
small GTP - binding proteins Ras and
Rho , and to the disruption , or depletion , of
cholesterol rich membrane micro - domains ( membrane rafts ) . -
pubmed
The inhibition of
mevalonate pathway by
statins impairs modification of
Ras and
Rho GTPases , which play key roles in signaling pathways related to
tumor formation ,
metastasis and cell
death . -
pubmed
In photoinhibited
photosystem II particles
pheophytin photoreduction remains unimpaired . -
pubmed
We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between
chlorophyll P-680 and
pheophytin in the
photosystem II reaction center . -
pubmed
Photoelectron spectroscopy and computational modeling of
thymidine homodimer anions . -
pubmed
The intact
thymidine homodimer anion ( dT(2)(- ) ) was generated in the gas phase using an infrared desorption / photoemission source and recorded by a pulsed photoelectron spectrometer . -
pubmed
Here we report that the most stable anion radical
homodimer geometries observed in the PES are the anionic
nucleoside coordinated by the
O8 atom of
thymine to the
deoxyribose of the second neutral
nucleoside . -
pubmed
RUT - positive patients without a
post - treatment evaluation were invited for a
urea breath test . -
pubmed
In vitro cytocompatibility evaluation of
MGF - Ct24E chemically grafted and physically blended with
maleic anhydride modified
poly ( D , L - lactic acid ) . -
pubmed
As a growth repair factor ,
mechano - growth factor (
MGF ) and its 24
amino acid peptide analogs corresponding to the unique C - terminal E - domain (
MGF - Ct24E ) positively regulate bone regeneration . -
pubmed
MGF - Ct24E was introduced into the
poly ( D , L - lactic acid ) (
PDLLA ) to improve bone regeneration in our previous study . -
pubmed
MGF - Ct24E - grafted
PDLLA was chemically characterized and showed potential as a biofunctional polymer . -
pubmed
In this study , we evaluated the cytocompatibility of
MGF - Ct24E chemically grafted and physically blended with
maleic anhydride modified
PDLLA , relative to
maleic anhydride modified
PDLLA (
MPLA ) as the control . -
pubmed
Electro - assisted solid - phase microextraction based on
poly ( 3,4 - ethylenedioxythiophen ) combined with
GC for the quantification of tricyclic antidepressants . -
pubmed
In this study , a
platinum wire coated with
poly ( 3,4 - ethylenedioxythiophen ) was used as an electro - assisted solid - phase microextraction fiber for the quantification of tricyclic antidepressant drugs in biological samples by coupling to
GC employing a flame ionization detector . -
pubmed
Group I metabotropic glutamate receptors in the medial prefrontal cortex : role in mesocorticolimbic
glutamate release in
cocaine sensitization . -
pubmed
One , 7 , or 21 days from the fourth injection , dual - probe microdialysis experiments were performed wherein Group I
metabotropic glutamate receptor agonist
DHPG was infused into the medial prefrontal cortex and
glutamate levels in this region as well as the nucleus accumbens or ventral tegmental area were examined . -
pubmed
The binding of
CXCL12 to
CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis , cell survival and/or proliferation , increase in intracellular
calcium , and gene transcription . -
pubmed
Extensive laboratory workup revealed low levels of serum
cortisol ( and its precursors
17alpha - hydroxyprogesterone and
11 - deoxycortisol ) , adrenal
androgens (
dehydroepiandrosterone sulfate and
androstenedione ) , and
estrogen (
estradiol ) ; and high levels of
mineralocorticoids precursors (
11 - deoxycorticosterone and
corticosterone ) with high levels of
ACTH ,
FSH and
LH . -
pubmed
Of the 62
rifampin - monoresistant samples by
LPA , 38 ( 61.4 % ) showed
rifampin resistance , while 21 ( 33.8 % ) were found susceptible to
rifampin by Xpert
MTB /
RIF using cartridge version
G4 . -
pubmed
Of the 116 pan - susceptible samples , only 83 were available for Xpert
MTB /
RIF testing ; 4 ( 5.1 % ) were
rifampin resistant , 74 ( 94.8 % ) were susceptible , and 5 ( 6.0 % ) showed an error . -
pubmed
The MGIT960 results showed 100 % agreement with
LPA results but only 64.4 % agreement with Xpert
MTB /
RIF results . -
pubmed
Sequencing analysis of discrepant samples showed 91.3 % concordance with
LPA but only 8.7 % concordance with the Xpert
MTB /
RIF assay . -
pubmed
Molecular mechanisms of
methoctramine binding and selectivity at
muscarinic acetylcholine receptors . -
pubmed
Methoctramine (
N , N '- bis [ 6 -[[( 2 - methoxyphenyl )- methyl ] hexyl ]- 1,8 - octane ] diamine ) is an M(2)-selective competitive antagonist of
muscarinic acetylcholine receptors and exhibits allosteric properties at high concentrations . -
pubmed
To reveal the molecular mechanisms of
methoctramine binding and selectivity we took advantage of reciprocal mutations of the
M ( 2 ) and M ( 3 ) receptors in the second and third extracellular loops that are involved in the binding of allosteric ligands . -
pubmed
To this end we performed measurements of kinetics of the radiolabeled antagonists
N - methylscopolamine (
NMS ) in the presence of
methoctramine and its precursors , fluorescence energy transfer between
green fluorescent protein - fused receptors and an
Alexa-555 - conjugated precursor of
methoctramine , and simulation of molecular dynamics of
methoctramine association with the receptor . -
pubmed
We confirm the hypothesis that
methoctramine high - affinity binding to the
M ( 2 ) receptors involves simultaneous interaction with both the orthosteric binding site and the allosteric binding site located between the second and third extracellular loops . -
pubmed
Methoctramine can bind solely with low affinity to the allosteric binding site on the extracellular domain of
NMS - occupied
M ( 2 ) receptors by interacting primarily with
glutamate 175 in the second extracellular loop . -
pubmed
In this mode ,
methoctramine physically prevents dissociation of
NMS from the orthosteric binding site . -
pubmed
Our results also demonstrate that
lysine 523 in the third extracellular loop of the
M ( 3 ) receptors forms a
hydrogen bond with
glutamate 219 of the second extracellular loop that hinders
methoctramine binding to the allosteric site at this receptor subtype . -
pubmed
Impaired interaction with the allosteric binding site manifests as low - affinity binding of
methoctramine at the
M ( 3 ) receptor . -
pubmed
The level of
H2S in serum , the activity of
superoxide dismutase (
SOD ) and the content of
malondialdehyde (
MDA ) , as well as anti -
superoxide anions in vascular tissue were determined by spectrophotometry . -
pubmed
Angiotensin II (
AngII ) levels in plasma were measured using competitive enzyme immunoassay . -
pubmed
Decreased vascular endothelial exfoliative cells and vascular smooth muscle cell ( SMC ) proliferation were shown in
NaHS groups by
HE staining . -
pubmed
Masson staining analysis showed reduced relative contents of
collagen fibers ( P & lt ; 0.05 ) and SMC ( P & lt ; 0.05 ) in
NaHS groups . -
pubmed
In the
D - gal group , the concentration of
AngII in plasma was significantly increased compared with that in vehicle group , while it was decreased in
NaHS groups ( P & lt ; 0.05 ) . -
pubmed
Moreover , levels of vascular tissue anti -
superoxide anion and the activity of
SOD were obviously higher ,
MDA was significantly lower in all
NaHS treated groups than those in the
D - gal group respectively ( P & lt ; 0.05 ) . -
pubmed
Western blot analysis showed that the expression of
AT1R was increased in
D - gal group compared with that in vehicle group , while it was decreased after treatment with
NaHS compared with that in
D - gal group ( P & lt ; 0.05 ) . -
pubmed
These results suggest that exogenous
H2S can ameliorate the age - related changes of aortic morphology , decrease the concentration of
AngII in plasma , down - regulate the expression of
AT1R in vascular tissue , and mitigate the level of oxidative stress . -
pubmed
Evaluation of the Xpert
MTB /
RIF assay for diagnosis of
tuberculosis and
rifampin resistance in county - level laboratories in Hunan province , China . -
pubmed
For the detection of
rifampin resistance , the sensitivity of
MTB /
RIF RIF - resistance detection was 92.9 % , and the specificity was 98.7 % . -
pubmed
Of the 26 Xpert
MTB /
RIF - positive and
RIF - resistant patients confirmed by LJ proportion tests , 20 ( 76.9 % ) patients were infected by
MDR - TB . -
pubmed
We found higher levels of
8 - OHdG and
PC in the NTD group than in the control group ; however , we did not observe a statistically significant difference in
8 - iso - PGF2alpha levels between the
NTD and the control groups . -
pubmed
NTD risk increased with increasing quartiles of
8 - OHdG [ odds ratio ( OR)=1.17 ; 95 % confidence interval ( CI ) 0.39 - 3.51 ; OR=2.19 ; 95 % CI , 0.68 - 7.01 ; OR=3.70 ; 95 % CI , 1.30 - 10.51 , for the second , third , and fourth quartile relative to the lowest quartile , respectively ; P=0.009 ] , and with increasing quartiles of PC ( OR=2.26 ; 95 % CI , 0.66 - 7.69 ; OR=3.86 ; 95 % CI , 1.17 - 12.80 ; OR=5.98 ; 95 % CI , 1.82 - 19.66 , for the second , third , and fourth quartile relative to the lowest quartile , respectively ; P=0.002 ] . -
pubmed
As expected ,
TNF - alpha increased
ET-1 and
ETA expression in a dose - dependent manner ; furthermore , application of the
TNF - alpha inhibitor
CAY10500 partially inhibited
hypoxia - induced
ET-1 and
ETA expression . -
pubmed
Expression of
STAT1 was inhibited by administration of 50muM
fludarabine (
2 - fluoro - ara - AMP ) for 2 , 4 , 8 , 24 , 48 or 72h or by using small interfering RNA ( siRNA ) . -
pubmed
Fludarabine inhibits the so induced phosphorylation of
STAT1 when administered 48 or 72h before stimulation . -
pubmed
Fludarabine reduces
STAT1 DNA - binding capacity . -
pubmed
Suppression of
STAT1 by
fludarabine or siRNA influences activity of
ERK1 / 2 which indicates a crosstalk between both pathways . -
pubmed
In this study , quantitative real - time
polymerase chain reaction analyses revealed that venom allergen 5 was significantly overexpressed in adult females of both
deltamethrin - resistant laboratory populations and two field populations . -
pubmed
The overexpression of
venom allergen 5 decreased the susceptibility of mosquito cells to
deltamethrin , while knockdown of this gene by RNAi increased the susceptibility of mosquitoes to
deltamethrin . -
pubmed
Treating larval or adult worms with antifungal
azole CYP450 inhibitors results in worm
death at low micromolar concentrations . -
pubmed
In addition , combinations of SmCYP450 - specific dsRNA and
miconazole show additive schistosomicidal effects supporting the hypothesis that
SmCYP450 is the target of
miconazole . -
pubmed
LPO ,
SOD ,
CAT and
GST levels were significantly higher in liver and kidney , whereas
GSH levels declined significantly compared to fish from the reference site . -
pubmed
For volatile profile , a total of 29 volatile compounds were identified by
HP - SPME -
GC -
MS , mainly
alcohols ,
esters ,
aldehydes ,
ketones and
phenols . -
pubmed
Solution Structure and Constrained Molecular Dynamics Study of
Vitamin B12 Conjugates of the Anorectic Peptide
PYY(3 - 36 ) . -
pubmed
Vitamin B12 -peptide conjugates have considerable therapeutic potential through improved pharmacokinetic and/or pharmacodynamic properties imparted on the peptide upon covalent attachment to
vitamin B12 (
B12 ) . -
pubmed
Chronic
estradiol treatment did not affect ovarian blood flow at
rest , while changed the basal ovarian
estradiol secretion rate , i.e. -
pubmed
RESULTS : MSG and
MSH groups had elevated blood pressure ,
triglyceride , waist circumference and fasting
glucose values in comparison to
gingivitis and healthy groups ( P<0.0001 ) . -
pubmed
Amino acids desmosine and
isodesmosine are unique cross - links in mature
elastin fibers and can serve as biomarkers of
elastin degradation when measured in body fluids . -
pubmed
Synthesis and pre - clinical evaluation of a new class of high - affinity
18F - labeled
PSMA ligands for detection of
prostate cancer by PET imaging . -
pubmed
PURPOSE : Current clinical imaging of
PSMA - positive
prostate cancer by positron emission tomography ( PET ) mainly features
68Ga - labeled tracers , notably
[ 68Ga ] Ga - PSMA - HBED - CC . -
pubmed
We aimed to develop high - affinity
PSMA inhibitors labeled with
fluorine-18 as alternative tracers for
prostate cancer . -
pubmed
, and co - administration of
PMPA was used to determine whether uptake was
PSMA - specific . -
pubmed
The synthesized bioavailable
curcuminoid formulation Cureit was characterizations by Nuclear magnetic resonance spectroscopy ( NMR ) , scanning electron microscopy ( SEM ) , X - ray diffraction ( XRD ) , Fourier transform infra - red ( IR ) , current - voltage ( I - V ) study , Quadrupole Time - of - Flight Mass Spectrometry ( Q -
TOF ) , differential scanning calorimeter ( DSC ) and thermogravimetric analysis (
TGA ) . -
pubmed
The chemical profile of Cureit was analyzed by Q -
TOF confirmed the presence of
curcuminoids (
curcumin ,
demethoxycurcumin and
bismethoxycurcumin ) ,
lactones ,
sesquiterpenes and their derivatives derived from polar layer ,
aromatic turmerone ,
dihydroturmerone ,
turmeronol ,
curdione and
bisacurone derived from non - polar layer . -
pubmed
IR , XRD , DSC and
TGA also confirmed the presence of
curcuminoids with high stability in the PNS formulation . -
pubmed
In
Wilson disease (
WD ) defective
AT7B function leads to biliary
copper excretion and pathologic
copper accumulation , particularly in liver and brain , where it induces cellular damage . -
pubmed
Mechanistically , chronic
alcohol consumption decreased the number of antitumor
CD8 + T cells but increased the number of tumor - associated macrophages ( TAMs ) in the liver in
DEN - initiated tumorigenesis . -
pubmed
Moreover , chronic
alcohol consumption aggravated
inflammation ,
fibrosis , and epithelial - mesenchymal transition (
EMT ) in the
pathological process of
HCC . -
pubmed
BACKGROUND : The barrier dysfunction in
atopic dermatitis (
AD ) skin correlates with stratum corneum ( SC ) lipid abnormalities including reduction of global lipid content , shorter
ceramide ( CER ) as well as
free fatty acid ( FFA ) chain length and altered
CER subclass levels . -
pubmed
AIM : We investigated whether the expression of CER and
FFA biosynthesis enzymes are altered in
AD skin compared with control skin and determine whether changes in enzyme expression can be related with changes in lipid composition . -
pubmed
These enzymes include
stearoyl CoA desaturase (
SCD ) ,
elongase 1 (
ELOVL1 ) and
ELOVL6 involved in
FFA synthesis and
beta - glucocerebrosidase (
GBA ) ,
acid - sphingomyelinase (
aSmase ) ,
ceramide synthase 3 (
CerS3 ) involved in CER synthesis . -
pubmed
This was accompanied by functional changes displayed by increased unsaturated
FFAs (
SCD ) and reduced FFA C22 - C28 (
ELOVL1 ) in
AD lesional skin . -
pubmed
The CER composition in
AD lesional skin showed corresponding changes such as increased CER
AS and
NS (
aSmase ) and decreased esterified
omega - hydroxy CERs ( CerS3 ) . -
pubmed
METHODS : The MAP consists of mandatory pre - operative
etoricoxib , intra - operative
acetaminophen , and
post - operative
acetaminophen with optional
post - operative
tramadol . -
pubmed
Assignment of the chemical shifts of the backbone atoms (
HN , N , CA , CB , and C ) of
proteins is often a prerequisite to using NMR information in the study of
proteins . -
pubmed
Furthermore , molecular docking studies suggested that 4h binds to the
colchicine binding site of
tubulin . -
pubmed
Cyclodipeptide c(
Orn - Pro ) Conjugate with
4 - Ethylpiperic Acid Abrogates
Cancer Cell Metastasis through Modulating
MDM2 . -
pubmed
The present work describes the synthesis , characterization , and anticancer properties of
c ( Lys - Pro ) ,
P1 ;
c ( Orn - Pro ) ,
P2 ; and conjugates
PA - c ( Lys - Pro ) ,
C1 ;
PA - c ( Orn - Pro ) ,
C2 ;
EPA - c ( Lys - Pro ) , C3 ; and
EPA - c ( Orn - Pro ) ,
C4 . -
pubmed
RESULTS :
TNF - alpha - induced
TSLP expression was inhibited by the
EGFR kinase inhibitor
AG1478 . -
pubmed
While
TSLP expression was induced by
EGF , it was inhibited by the
MEK inhibitor ,
U0126 . -
pubmed
The open reading frames of
RcWRI1 - A ( 1341 bp ) and RcWRI1 - B ( 1332 bp ) differ by a stretch of 9 bp , such that the predicted
RcWRI1 - B lacks the three
amino acid residues " VYL " that are present in
RcWRI1 - A. -
pubmed
When the two isoforms were individually introduced into an
Arabidopsiswri1 - 1 loss - of - function mutant , total
fatty acid content was almost restored to the wild - type level , and the percentage of the wrinkled seeds was largely reduced in the transgenic lines relative to the
wri1 - 1 mutant line . -
pubmed
Both
RcWRI1 - A and
RcWRI1 - B can be used as a key transcriptional regulator to enhance
fatty acid and oil biosynthesis in leafy biomass . -
pubmed
Anti -
cancer Effects of
HNHA and
Lenvatinib by the Suppression of
EMT - Mediated Drug Resistance in
Cancer Stem Cells . -
pubmed
This study aimed to develop targeted agents against
thyroid malignancy , focusing on individual and synergistic effects of HNHA (
histone deacetylase ) ,
lenvatinib (
FGFR ) , and
sorafenib (
tyrosine kinase ) inhibitors . -
pubmed
HNHA -
lenvatinib combined treatment induced markers of cell cycle arrest and apoptosis and suppressed anti - apoptosis markers , epithelial - mesenchymal transition (
EMT ) , and the
FGFR signaling pathway . -
pubmed
HNHA -
lenvatinib combination treatment thus blocked the
FGFR signaling pathway , which is important for
EMT . -
pubmed
ANOVA showed that class of film , fungal species , aw and temperature and their interactions significantly affected growth rates (
GR ) , ED50 and ED90 and the doses for total fungal growth inhibition and
OTA production . -
pubmed
Depending on the species , aw and temperature affected
GR and
OTA production in a different way . -
pubmed
Selenoprotein P (
SeP ) , a liver - derived secretory protein , functions as a
selenium supply protein in the body . -
pubmed
Cellular assays were carried out for chemotaxis (
agarose spot assay ) , cell motility ( live cell imaging ) , metabolism (
MTT ) and cell polarisation (
CK18 ;
ezrin ) . -
pubmed
Several analogues of
( + ) - ursolic acid ( 1 ) were synthesized , and a preliminary structure - activity relationship (
SAR ) study indicated that the
C-3 hydroxy and C-28
carboxylic acid groups and
19,20 - dimethyl substitution are all essential in the mediation of the bioactivities observed for this
triterpenoid . -
pubmed
A multivariate logistic regression analysis was used to examine the effects of sociodemographic characteristics , lifestyle , activity status , physical exercise ,
systolic blood pressure ,
low - density lipoprotein (
LDL ) ,
high - density lipoprotein (
HDL ) ,
triglycerides ,
body mass index (
BMI ) , and
CRP levels on
depressive symptoms . -
pubmed
After adjusting for age , education , marital status , hukou , residence , cigarette smoking ,
alcohol drinking , eating meals , activity status , major
accidental injury , diseases , health status , physical exercise ,
systolic blood pressure ,
LDL ,
HDL ,
triglycerides , and
BMI ,
depressive symptoms were not associated with subsequent high CPR levels among the middle - aged and elderly (
CRP 1.01 - 3.00 mg / L : OR = 0.93 , 95 % CI = 0.83 - 1.03 ;
CRP 3.01 - 10.00 mg / L : OR = 0.95 , 95 % CI = 0.82 - 1.10 ;
CRP & gt;10 mg / l : OR = 1.11 , 95 % CI = 0.88 - 1.39).Our data do not support an association between
CRP and
depressive symptoms in both middle - aged and elderly men and women among china . -
pubmed
The role of TDM in optimizing
vedolizumab ,
ustekinumab , and
tofacitinib remains unclear , given differences in pharmacokinetics and immunogenicity compared to
TNF antagonists . -
pubmed
In addition to the original models for CID and
HCD fragmentation , we have added specialized models for the TripleTOF 5600 + mass spectrometer , for
TMT - labeled peptides , for iTRAQ - labeled peptides , and for iTRAQ - labeled phosphopeptides . -
pubmed
Molecular dynamics ( MD ) revealed that the root mean - square deviation ( RMSD ) , root mean square fluctuation ( RMSF ) , radius of gyration ( Rg ) , and the number of
hydrogen and hydrophobic bonds between TQ and residues of
BCL-2 ,
BCL - XL and MCL-1were significantly ( P<0.001 ) changed . -
pubmed
Background : The
retinol binding protein 4 (
RBP4 ) is essential in delivering
retinol to the retinal pigment epithelium and normal functioning of the visual cycle . -
pubmed
Acetonyl Peroxy and
Hydro Peroxy Self- and Cross- Reactions : Kinetics , Mechanism , and
Chaperone Enhancement from the Perspective of the
Hydroxyl Radical Product . -
pubmed
After treatment with raw cornstarch , the total
triglycerides (
TG ) level has significantly decreased by 30 + -50 % ( 8.37 + -7.23 to 5.39 + -5.29 mmol / L , p<0.001 ) . -
pubmed
Oxygen saturation was evaluated at baseline ( T0 ) , just after the arterial embolization (
T1 ) , at 10 minutes (
T2 ) , at 20 minutes ( T3 ) , and at 40 minutes ( T4 ) . -
pubmed
We quantified brain cell
death using serum levels of biomarkers of glial and neuronal cell
death (
Glial Fibrillary Acidic Protein (
GFAP ) ,
Ubiquitin Carboxy - terminal Hydrolase - L1 (
UCH - L1 ) ,
S100B and
Alpha II Spectrin Breakdown Product 150 (
SBDP ) ) in the Biomarkers of
Injury and Outcome -
Progesterone for
Traumatic Brain Injury , Experimental Clinical Treatment ( BIO - ProTECT ) study . -
pubmed
Serum
progesterone levels were measured at 24 and 48 hours
post -
injury . -
pubmed
Similarly , at 24 and 48 hours
post -
injury , there were no differences in biomarker levels in the
progesterone versus placebo groups ( p>0.15 ) . -
pubmed
We conclude that
progesterone treatment does not decrease the levels of biomarkers of glial and neuronal cell
death during the first 48 hours
post -
injury . -
pubmed
Coupled porosity and heterojunction engineering :
MOF - derived porous
Co3O4 embedded on
TiO2 nanotube arrays for water remediation . -
pubmed
METHODS : This cross - sectional , case - control study evaluates associations among OC mucus inflammatory proteins , psychophysical olfactory testing , and computed tomography (
CT ) analysis of the OC and
sinuses . -
pubmed
At
rest , the heart rate was significantly lower on
metoprolol than on
practolol or H 87/07 . -
pubmed
On the contrary the correlation of total and free
thyroid hormone concentrations with the response of
TSH to
TRH was not significant . -
pubmed
The
amino acid sequence of
polypeptide II from beef heart cytochrome c oxidase is described . -
pubmed
Thus subunit II of the
oxidase is a member of this
copper protein family . -
pubmed
Thus subunit
II is like a blue
copper protein with type I
copper . -
pubmed
In further study of these specimens , an immunoperoxidase staining technique was used to stain for
hCG in
formalin - fixed sections . -
pubmed
Eserine sulphate ( 10(-5 ) M ) was used as inhibitor for
AChE. -
pubmed
Ganglioside - induced
CD4 endocytosis occurs independent of
serine phosphorylation and is accompanied by dissociation of
P56lck . -
pubmed
Gangliosides induce a selective and complete modulation of
CD4 from the surface of T cells . -
pubmed
This process is independent of
serine phosphorylation of the
cytoplasmic tail of
CD4 and does not require the association between the
tyrosine protein kinase p56lck and the
cytoplasmic tail of
CD4 . -
pubmed
Ganglioside - induced
CD4 endocytosis is accompanied by the loss of
p56lck activity associated with
CD4 . -
pubmed
The kinetics of
p56lck dissociation after
ganglioside treatment is identical to that of
CD4 endocytosis , suggesting that
p56lck is displaced in the process of endosome formation . -
pubmed
Gonadotropin - releasing hormone (
GnRH ) agonists show promise as new therapeutic agents with considerable potential for clinical applications in endocrinology , gynecology , pediatrics , and oncology . -
pubmed
GnRH agonists are derived from
GnRH . -
pubmed
In as much as
PBN contains mu and kappa but no detectable
delta receptors , the present results suggest that mu opioid activity within the PBN is involved in the mediation of feeding behavior . -
pubmed
DNA was isolated from the peripheral blood lymphocytes and aromatic DNA adducts were detected by
32P - postlabelling assay using the
nuclease P1 digestion procedure for the enrichment of the adducts . -
pubmed
Induction of
preterm labour by infusion of
ACTH ( 66.6 ng / min for 15 min every 2 h ) to the fetus resulted in a reduction in plasma
progesterone concentrations and increases in values of oestradiol-17 beta and
13,14 - dihydro 15 - keto PGF-2 alpha in maternal plasma . -
pubmed
When cell growth was inhibited with
mevinolin , a
cholesterol synthesis inhibitor , the addition of
LDL to the medium had no effect on the growth of cells from five of six
tumor cell lines . -
pubmed
Thus , five of six
colon tumor cell lines were unable to overcome a
mevinolin block in
cholesterol metabolism indicating that these cells were deficient in
LDL receptors . -
pubmed
Plasma
atrial natriuretic peptide was increased and was not significantly modified by infusion of hypertonic saline or acute administration of
furosemide . -
pubmed
In the neutral extracts an immunoreactive
somatostatin of unidentified molecular structure dominated quantitatively over
somatostatin 14 and 28 , which were the major components in
acetic acid extracts . -
pubmed
The recent availability of monoclonal antibodies immunoreactive to
T - or B - lineage antigens in
formalin - fixed ,
paraffin - embedded tissue has permitted the adaptation of frozen - section immunodiagnostic criteria to paraffin - embedded tissue . -
pubmed
Arginine vasopressin increases the
IR - ACTH response to
oCRF fourfold when given simultaneously with
oCRF . -
pubmed
Salivary
lysozyme ,
peroxidase , and
hypothiocyanite concentrations were already at the adult level at the time when the primary teeth erupt , whereas
immunoglobulin (
IgA ,
IgG , and
IgM ) ,
lactoferrin ,
myeloperoxidase , and
thiocyanate concentrations were significantly lower in children than in adults . -
pubmed
Dentate children had more
IgG ,
thiocyanate , and protein in whole saliva than did predentate children . -
pubmed
In both anemic and nonanemic patients serum
ferritin levels decreased and total
iron - binding capacity increased as gestation progressed . -
pubmed
The total
iron - binding capacity is higher in maternal than in umbilical cord blood at delivery ; this suggests that little or no
ferritin or
transferrin is transferred from mother to fetus . -
pubmed
The removal of
transferrin from the surface of the trophoblast by
thiocyanate and its rebinding were demonstrated . -
pubmed
Creatine and the control of
myosin synthesis in differentiating skeletal muscle . -
pubmed
Skeletal muscle cells formed both in vitro and in vivo synthesize
myosin heavy chain faster when supplied
creatine in vitro . -
pubmed
Gamma - glutamyltransferase activity was present in very few of the spontaneous
neoplasms or in the
neoplasms induced in the absence of
phenobarbitone administration . -
pubmed
GA and HS selectively inhibited the
DMPP - induced contraction without having an antagonistic effect on the
McN - A-343 - induced and
acetylcholine - induced contraction . -
pubmed
In addition , since
GA and HS showed a local anesthetic action weaker than that of
procaine , the effect of
procaine was studied on the same preparation . -
pubmed
This same component accumulates label at an accelerated rate in the presence of the
protease inhibitor leupeptin . -
pubmed
The proarrhythmic effects of the
ATP - sensitive potassium channel modulators
cromakalim ( n = 10 ; 0.01 to 0.3 mg / kg i.v . ) -
pubmed
; n = 7 ) , or
Vasopressin (
VP : 0.8 U / kg B.W. -
pubmed
The response of forearm blood flow to
sodium nitroprusside was greater when subjects received a high
sodium diet than when they received a low
sodium diet ( F = 7.11 , P & lt ; .05 ) ; however , the response to
methacholine was not altered by
sodium intake ( F = 0.57 , P =
NS ) . -
pubmed
However , increasing resistance to
ceftazidime and other third generation
cephalosporins , particularly among Enterobacteriaceae , due to the emergence of plasmid - mediated extended spectrum
beta - lactamases and the class I chromosomally mediated
beta - lactamases , is of concern . -
pubmed
We have used intravenous
gammaglobulin (
IVIG ; 10 %
Gamimune N ) to determine both its efficacy in reducing panel - reactive antibodies in vitro and the prognostic value of the in vitro testing for in vivo efficacy . -
pubmed
Biochemical studies to determine the effective component of IVIG show that it is the
IgG fraction and not soluble
antigen or the minor
IgM or
IgA contaminants that is responsible . -
pubmed
An increase in [
Ca2+]i in neurons
B5 was also induced by a
PTH agonist [
bPTH -( 1 - 34 ) ] , but not by a
PTH antagonist [
bPTH -( 3 - 34 ) ] . -
pubmed
Furthermore , our results demonstrated that the effect of
PTH on [
Ca2 +]i in neurons
B5 was not due to the hormonal modulation of voltage - dependent
Na + or
K + channels or a
Na +/ Ca2 + exchanger . -
pubmed
Serum
cortisol concentration was measured before and after adrenocortical stimulation with 100 IU of
adrenocorticotropic hormone , 1 week prior to TA administration , and 4 and 8 weeks thereafter . -
pubmed
Both compounds resulted in decreased binding of
conA and
WGA to cell surface
oligosaccharides showing they were causing a reduction in N - linked cell surface
glycoproteins . -
pubmed
Changes in the plasma concentration of
adrenaline were produced by varying the rate of an intravenous infusion of
adrenaline and in the effects of
5 - HT , by intravenous injections of the selective
5 - HT2 receptor antagonist ,
ICI 170809 . -
pubmed
Antagonism of the
5 - HT2 receptor by
ICI 170809 , in the absence of an infusion of
adrenaline , abolished
thrombus formation ( mean ED50 0.41 microgram kg-1 , i.v . ) -
pubmed
The locations of
[ 3H ] thymidine - labeled neurons were analysed by autoradiography eight to 16.5 months
post - grafting . -
pubmed
N - Ethylmaleimide (
NEM ) , an inhibitor of
H (+)- ATPase , reduced the cellular pH ( pHi ) dose - dependently . -
pubmed
1 x 10(-7 ) M
AVP also increased [
Ca2+]i from 111.2 to 189.3 nM , which was significantly reduced by
NEM in a dose - dependent manner . -
pubmed
These results indicate that
H (+)- ATPase participates the cellular action of
AVP mediated via the pHi control in renal papillary collecting tubule cells . -
pubmed
Liver and kidney
GST and liver
GR activities were significantly increased after
AAF administration . -
pubmed
Kidney
GPx activities were significantly affected ; activity assayed with
cumene hydroperoxide (
cu - OOH ) was increased but activity assayed with
H2O2 was reduced . -
pubmed
Design and synthesis of
C - linked fucosides as inhibitors of
E - selectin . -
pubmed
Two series of
C - linked fucosides as mimetics for the
tetrasaccharide sialyl Lewis X have been synthesized and tested as inhibitors of
E - Selectin . -
pubmed
In addition ,
BP170 was co - expressed with
tyrosine hydroxylase which suggests its possible role in the
dopamine pathway . -
pubmed
Differential effects of
gonadotropin - releasing hormone (
GnRH )
pulse frequency on
gonadotropin subunit and
GnRH receptor messenger ribonucleic acid levels in vitro . -
pubmed
The hypothalamic hormone ,
GnRH , is released and transported to the anterior pituitary in a pulsatile manner , where it binds to specific high - affinity receptors and regulates
gonadotropin biosynthesis and secretion . -
pubmed
The frequency of
GnRH pulses changes under various physiological conditions , and varying
GnRH pulse frequencies have been shown to regulate differentially the secretion of
LH and
FSH and the expression of the
gonadotropin alpha , LH beta , and
FSH beta subunit genes in vivo . -
pubmed
Cells were treated with 10 nM
GnRH pulses for 24 h at a frequency of every 0.5 , 1 , 2 , or 4 h. alpha , LH beta , and
FSH beta messenger RNA ( mRNA ) levels were increased by
GnRH at all
pulse frequencies alpha and
LH beta mRNA levels and
LH secretion were stimulated to the greatest extent at a
GnRH pulse frequency of every 30 min , whereas
FSH beta mRNA levels and
FSH secretion were stimulated maximally at a lower
GnRH pulse frequency , every 2 h.
GnRH receptor (
GnRHR ) mRNA levels also were increased by
GnRH at all
pulse frequencies and were stimulated maximally at a
GnRH pulse frequency of every 30 min . -
pubmed
This suggests that alpha and LH beta mRNA levels are maximally stimulated when
GnRHR levels are relatively high , whereas
FSH beta mRNA levels are maximally stimulated at lower levels of
GnRHR expression , and that the mechanism for differential regulation of the gonadotropins by varying
pulse frequencies of
GnRH may involve levels of
GnRHR . -
pubmed
Furthermore , these data suggest that the mechanisms whereby varying
GnRH pulse frequencies stimulate
alpha LH beta , and
GnRHR gene expression are similar , whereas the stimulation of FSH beta mRNA levels may be different . -
pubmed
Most parasympathetic axons contained IR for
vasoactive intestinal peptide (
VIP ) and
neuropeptide Y (
NPY ) , with or without
enkephalin (
Enk ) . -
pubmed
PURPOSE : Our experience with
IVF using low - dose
clomiphene citrate for stimulation in " non- " and " poor " responders was reviewed and the treatment outcomes with the previous controlled ovarian stimulation cycles in which
hMG and
GnRH agonist were used were compared . -
pubmed
METHODS : The treatment outcome in 11 non- and 20 poor responders having 30 and 53
clomiphene citrate IVF treatment cycles , respectively , were compared with the treatment outcome in the previous long - protocol
buserelin /
hMG cycles . -
pubmed
RESULTS : The clinical pregnancy rates per oocyte collection achieved in the first
clomiphene citrate cycle in non ( 9.1%)- and poor ( 10 % ) responders were comparable to those achieved by poor responders ( 11.9 % ) who had
buserelin /
hMG using the long protocol . -
pubmed
CONCLUSIONS : IVF using long - protocol
buserelin /
hMG is more successful than using
clomiphene citrate stimulation . -
pubmed
However , this advantage may not be significant in those women with a previous poor response to
buserelin /
hMG . -
pubmed
It is suggested that for such poor responders , three attempts of
IVF in a
clomiphene citrate cycle may offer a viable therapeutic alternative before reverting to more stressful , expensive , and time - consuming treatment . -
pubmed
Flavopiridol is known to inhibit potently the activity of multiple
cyclin - dependent kinases . -
pubmed
Flavopiridol , when administered as daily bolus intravenous (
IV ) injections , produced selective apoptosis of cells in the thymus , spleen , and lymph nodes , resulting in
atrophy of these organs . -
pubmed
After treatment with 7.5 mg / kg
flavopiridol bolus
IV or intraperitoneal on each of 5 consecutive days , 11 out of 12 advanced stage subcutaneous ( s.c . ) -
pubmed
We discovered that compounds with 26 membered rings of
[ Cys4,D - Nal7,Cys11 ] alpha - MSH(4 - 11 ) displayed specific
MC4 receptor selectivity . -
pubmed
The preference order of the different
MC receptor subtypes for the novel
[ Cys4D - Nal7Cys11 ] alpha - MSH(4 - 11 ) analogues are distinct from all other known
MSH analogues , particularly as they bind the
MC4 receptor with high and the
MC1 receptor with low relative affinities . -
pubmed
HS964 and
HS014 have 12 and 17 fold
MC4 / MC3 receptor selectivity , respectively , which is much higher than for the previously described
cyclic lactam and
[ Cys4,Cys10 ] alpha - MSH analogues
SHU9119 and
HS9510 . -
pubmed
HS964 is the first substance showing higher affinity for the
MC5 receptor than the
MC1 receptor . -
pubmed
We found that a compound with a 29 membered ring of
[ Cys3,Nle10,D - Nal7,Cys11 ] alpha - MSH(3 - 11 ) (
HS010 ) had the highest affinity for the
MC3 receptor . -
pubmed
In order to improve both quantitative and qualitative
thalassemia erythropoiesis , several trials were undertaken combining
rHuEPO with
hydroxyurea (
HU ) , which is known to increase %
Hb F , MCV and MCH without a major effect on
Hb levels . -
pubmed
The designed trial included 3 to 6 months of
HU alone ( 20 mg / kg x 4 / week ) , or with
rHuEPO alone ( 500 u / kg x 3 / week or 375 u / kg x 2 / week ) or a combination of the two drugs . -
pubmed
It is not known whether the addition of oral
iron to
rHuEPO is warranted for maximal erythropoietic response . -
pubmed
Calcium - binding proteins , such as
S-100 , dimerize readily , and this phenomenon plays an important role in their regulation of target enzymes [ Krebs , J. , Quadroni , M. & amp ; Van Eldik , L.J. -
pubmed
We have investigated by Fourier - transform ion cyclotron resonance ( FTICR )
MS the conformational states of the
calcium - binding protein
calmodulin , and present clear evidence for a
calmodulin dimer formed as a result of noncovalent interactions between folded monomers . -
pubmed
With the use of denaturing solutions ( 1 : 1
acetonitrile /
water + 1 %
formic acid ) , relatively high charge states ( 20 & lt ; z & lt ; 10 ) of monomeric
calmodulin ions were detected , whereas when
calmodulin was electrosprayed from buffer , monomers ions with only 5 - 10 charges were detected . -
pubmed
CD measurements for
calmodulin in buffered solution revealed that its alpha - helical content was significantly higher than that for
calmodulin in
acetonitrile / water solutions , consistent with a proposition that changes in charge state distributions observed in the
MS experiments reflect differing states of
calmodulin folding . -
pubmed
Superoxide dismutase activity and
reduced glutathione content were not significantly different in either cell type when challenged with Ox or Ox +
COM . -
pubmed
Effect of
lovastatin on
small GTP binding proteins and on
TGF - beta1 and
fibronectin expression . -
pubmed
It was postulated that this down - regulation may be linked to signaling of
small guanine triphosphate ( GTP )- binding proteins and mediated by the limitation of
isoprenoids such as
farnesylpyrophosphate (
FPP ) and
geranylgeranylpyrophosphate (
GGPP ) in RMC . -
pubmed
To determine the
isoprenoid and small
GTP - binding proteins involved in
TGF - beta1 and
fibronectin expression . -
pubmed
Suppression of
TGF - beta1 and
fibronectin expression by
lovastatin was reversed effectively when
GGPP was added alone . -
pubmed
Partial reversal of
lovastatin effect on
fibronectin and
TGF - beta1 expression was found when
FPP was added alone . -
pubmed
Adding both
GGPP and
FPP resulted in complete reversal of
lovastatin effect on
fibronectin but not
TGF - beta1 suggesting that
fibronectin and
TGF - beta1 are regulated differently . -
pubmed
Intracellular reduction of
selenite into
glutathione peroxidase . -
pubmed
Selenium (
Se ) in
selenite is present in an oxidized state , and must be reduced for it to be incorporated as
selenocysteine into selenoenzymes such as
glutathione peroxidase (
GPx ) . -
pubmed
In vitro ,
Se , as in
selenite , can be reduced utilizing
glutathione (
GSH ) and
glutathione reductase (
GRed ) . -
pubmed
Buthionine sulfoximine (
BSO ) was used to decrease
GSH levels ,
1,3 bis -( 2 chloroethyl )- 1 - nitrosourea (
BCNU ) was used to inhibit
GRed activity and
methylene blue (
MB ) was used to decrease cellular
NADPH levels . -
pubmed
This statistical method evaluates the effects of
BSO ,
BCNU , MB and
selenite alone and in combinations on
GPx activity . -
pubmed
For cells with abnormal ability to generate
NADPH , lowering the
GSH levels had a small effect on
selenite - dependent
GPx synthesis . -
pubmed
GRed activity is not required for the
selenite - dependent synthesis of
GPx . -
pubmed
All of the
dppA - disrupted mutants produced a normal level of native form of
DMSOR and grew by
DMSO respiration , indicating that the loss of
DppA protein alone had no effect on the formation of active
DMSOR . -
pubmed
Cocaine and
amphetamine regulated transcript ( CART ) peptide has been shown to be an anorectic peptide that inhibits both normal and
starvation - induced feeding and completely blocks the feeding response induced by
neuropeptide Y and regulated by
leptin in the hypothalamus . -
pubmed
The C - terminal part containing the three
disulfide bridges
CART(48 - 89 ) is the biologically active part of the molecule affecting food intake . -
pubmed
CART consists mainly of turns and loops spanned by a compact framework composed by a few small stretches of antiparallel beta - sheet common to
cystine knots . -
pubmed
1.4.3.6 ) , the
enzymes which control membrane properties (
LOX ) and the catabolism of
polyamines (
DAO ) , respectively . -
pubmed
Although
irbesartan had no effect on
glucose transport (
2 - deoxyglucose uptake ) in the epitrochlearis muscle , which consists mainly of type IIb fibers , acute
angiotensin II receptor antagonism led to a dose - dependent increase in
insulin action in the predominantly type I soleus muscle . -
pubmed
Irbesartan at 25 and 50 mg / kg induced significant increases ( 41 % and 50 % , respectively ; P<0.05 ) in
insulin - mediated
glucose transport . -
pubmed
Moreover , these acute
irbesartan - induced improvements in soleus - muscle
glucose transport were associated with enhancements in whole - body
insulin sensitivity ( r=-0.732 ; P<0.05 ) , as assessed during an oral
glucose tolerance test . -
pubmed
d(-1 ) ) ,
glucose tolerance was enhanced further , and
insulin - mediated
glucose transport was significantly elevated in both epitrochlearis ( 32 % ) and soleus ( 73 % ) muscle . -
pubmed
Chronic
irbesartan - induced increases in whole - body
insulin sensitivity were associated with increased
insulin - mediated
glucose transport in both epitrochlearis ( r=-0.677 ; P<0.05 ) and soleus ( r=-0.892 ; P<0.05 ) muscle . -
pubmed
In summary ,
angiotensin II receptor (
AT ( 1 )- subtype ) antagonism , either acutely or chronically , improves
glucose tolerance , at least in part because of an enhancement in skeletal - muscle
glucose transport , and the effect of chronic
angiotensin II receptor antagonism on type I skeletal - muscle
glucose uptake is associated with an increase in
GLUT-4 protein expression . -
pubmed
These results show that residues around the
NPA1 box can influence the folding of the
MIP in the membrane , and provide structural evidence for the poor
water transport properties of
MIP , as the
NPA boxes lie outside the plane of the membrane . -
pubmed
BACKGROUND :
Xanthurenic acid is an endogenous product of
tryptophan degradation by
indoleamine 2,3 - dioxygenase (
IDO ) . -
pubmed
In the control cells
gelsolin stained the perinuclear region , whereas in the presence of 10 microM
xanthurenic acid gelsolin is translocated to the cytoskeleton , but does not lead to cytoskeleton breakdown . -
pubmed
At low ( 5 to 10 microM ) of
xanthurenic acid concentration , the elongation of the cytoskeleton was associated with migration of mitochondria and
cytochrome c release . -
pubmed
We observed an induction of
calpain Lp 82 and an increase of free
Ca2 + in the cells in a
xanthurenic acid concentration - dependent manner . -
pubmed
Xanthurenic acid induces calpain
Lp82 and
caspases in the cells growing in the dark and can be involved in senile
cataract development . -
pubmed
PURPOSE : To review the uses and previously proposed mechanisms of action of amniotic membrane transplantation (
AMT ) and to suggest a new mechanism of action for the effectiveness of
AMT in resolving a persistent
epithelial defect (
PED ) . -
pubmed
Electron microscopic determination of
silver incorporation in
collagen fibers as a model of organic - metal chiral supramolecular structure with optical anisotropic properties . -
pubmed
The high positive linear dichroism (
LD ) exhibited by
silver - impregnated
collagen fibers are thought to be caused by an ordered binding of
silver nanoparticles to the
collagen fibers . -
pubmed
A correlation between
LD , established by image analysis , and the size of
silver particles as measured by electron microscopy was investigated in the same sections of
silver - impregnated tendon
collagen fibers . -
pubmed
Silver particles were found to be periodically ordered along the
collagen fibers , with some preference for the 67 nm
collagen period and
collagen a bands . -
pubmed
The finding that interference colors are correlated to abnormal dispersion of birefringence characteristics supports the concept that
collagen fibers induce an ordered binding of
silver distributed along the fibers . -
pubmed
Hepatocyte
prostaglandin synthesis and
Mdr1 expression . -
pubmed
The most common
ART was
zidovudine /
lamivudine ( 39 % ) followed by
zidovudine -
lamivudine -
nelfinavir ( 34 % ) , although 34 combinations were used . -
pubmed
TRP ,
5 - hydroxyindoleacetic acid (
5 - HIAA ) ,
kynurenine (
KYN ) , and
interleukin-6 (
IL-6 ) were measured . -
pubmed
A
chronic colitis was induced by oral administration of 5 %
dextran sulfate sodium (
DSS ) for 9 days followed by 2 % DSS for 18 days . -
pubmed
Catechol - O - methyltransferase (
COMT ) is an enzyme that inactivates
catecholamines , including
levodopa . -
pubmed
An
amino acid change (
Val-108 - Met ) in the
COMT protein has been found to result in a change from high to low enzyme activity . -
pubmed
Dynamic aspect of
bacteriorhodopsin as a typical membrane protein as revealed by site - directed solid - state
13C NMR . -
pubmed
We demonstrate here a general feature of dynamic aspect of membrane proteins as revealed by site - directed
13C NMR studies on
bacteriorhodopsin (
bR ) as a typical membrane protein and a variety of mutants at ambient temperature . -
pubmed
It is emphasized that the observed displacement of [ 3 - 13C]-labeled
Ala 184 peak at 17.4 ppm in the presence of kinked structure in this model peptide is consistent with that of intact
protein at 17.27 ppm . -
pubmed
Maintenance immunosuppression regimens after the first and second grafts were similar , consisting of a combination of a
calcineurin inhibitor ,
mycophenolate mofetil , and
prednisone . -
pubmed
We have previously shown that hormone therapy (
HT ) with
medroxyprogesterone acetate (
MPA ) alone or in combination with conjugated equine
estrogens ( CEE ) impairs
insulin sensitivity . -
pubmed
In the current study , we sought to determine if the effect of
MPA on whole body
insulin sensitivity is associated with alterations in
insulin signaling proteins in skeletal muscle . -
pubmed
There was a tendency for decreased
insulin receptor tyrosine phosphorylation with CEE +
MPA treatment ( ANOVA
P = 0.14 ) . -
pubmed
The cardiac slow delayed rectifier
potassium channel (
IKs ) , comprised of (
KCNQ1 ) and beta (
KCNE1 ) subunits , is regulated by sympathetic nervous stimulation , with activation of
beta - adrenergic receptors PKA phosphorylating IKs channels . -
pubmed
In IKs /
B2 - AR myocytes , IKs density was increased , and activation shifted in the hyperpolarizing direction ; IKs was not further modulated by exposure to
isoproterenol , and
KCNQ1 was found to be
PKA - phosphorylated . -
pubmed
Conversely ,
beta2 - AR overexpression did not affect
L - type calcium channel current ( ICaL ) under basal conditions with ICaL remaining responsive to
cAMP . -
pubmed
The G - allele carriers of the
SREBF-1 gene C - G polymorphism in exon 18c and coding for
glycine at the protein level ( G952 G ) have shown to associate more frequently with
obesity and
type 2 diabetes than the C - allele carriers . -
pubmed
Similarly muscle
cholesterol ( p=0.045 ) and
lathosterol ( p=0.054 ) concentrations were elevated in the CC homozygotes supporting the view that endogenous
cholesterol synthesis rate is
SREBF-1 genotype - dependent . -
pubmed
Metabolism of
levodopa via the enzyme
catechol - O - methyltransferase requires
S - adenosylmethionine (
SAM ) as a
methyl donor . -
pubmed
Plasma levels of
SAM and
levodopa significantly increased , but
MET concentrations did not significantly decrease . -
pubmed
RESULTS : The mean total
cholesterol (
TC ) ,
low - density lipoprotein cholesterol (
LDLC ) , and
triglyceride (
TG ) levels were similar in men and women , while the mean
HDLC level was significantly higher in women than men ( 58.3+/-18.1 vs. 50.3+/-13.5 mg / dL ; p=0.0064 ) . -
pubmed
piscifermentans ;
lactic acid production ,
urease activity , bacteriocin production and the antibiotic susceptibility of it were also determined . -
pubmed
Kinetics of hydrolysis of
acetylcholine and
acetylthiocholine by two types of
acetylcholinesterase and
butyrylcholinesterase inhibited by 13 new inhibitors ( 5
carbamates and
8 carbazates -- hydrazinium derivatives ) was measured in vitro in a batch reactor at 25 degrees C , pH 8 , ionic strength 0.11 M and
enzyme activity 3.5 U by four nondependent analytical methods . -
pubmed
Kinetics of hydrolyses inhibited by all studied
carbamates ,
sevin ,
carbazates ( with exceptions ) and
rivastigmin ( with exceptions ) can be simulated by the competitive inhibition model with irreversible reaction between
enzyme and inhibitor . -
pubmed
The tested
carbazates failed as inhibitors of
cholinesterases . -
pubmed
Although the mechanisms are not understood , evidence suggests that
17beta - estradiol (
E2 ) confers protection from
cardiovascular and renal complications in many diseases . -
pubmed
There was no change in
ERalpha or
AT1R binding following Cas + /-
DHT ( 25 mg ) treatment , although
Cas did elevate cortical
ERbeta ( P & lt ; 0.01 ) . -
pubmed
Interestingly , the high dose
DHT ( 200 mg ) elevated
ERalpha 150 % above intact levels and profoundly decreased
AT1R binding ( 1,824 + /- 705 cpm , P & lt ; 0.001 vs. intact male ) . -
pubmed
Furthermore ,
E2 regulates
ERalpha and is inversely associated with glomerular
AT1R binding , supporting our hypothesis that
E2 tonically suppresses
AT1Rs and suggesting a potential mechanism for the protective effects of
estrogen . -
pubmed
Correlations between the level of
fructosamine and other variables including the results of
glucose challenge test ( GCT ) , oral
glucose tolerance test ( OGTT ) , and
glycosylated hemoglobin (
HbA1c ) test , and infant 's
birth weight were analyzed by Pearson correlation . -
pubmed
Molecular insights into
nitrogenase FeMoco insertion -- the role of
His 274 and
His 451 of
MoFe protein alpha subunit . -
pubmed
The final step of
FeMo cofactor (
FeMoco ) assembly involves the insertion of
FeMoco into its binding site in the
molybdenum -
iron (
MoFe ) protein of
nitrogenase . -
pubmed
Our results from combined metal , activity ,
EPR , stability and insertion analyses show that mutations of
His alpha274 and/or
His alpha451 , two of the
histidines that belong to a so - called
His triad , to small uncharged
Ala specifically reduce the accumulation of
FeMoco in
MoFe protein . -
pubmed
Subunit H of the
vacuolar ( H + ) ATPase inhibits
ATP hydrolysis by the free
V1 domain by interaction with the rotary subunit F. The
vacuolar ( H + ) ATPases (
V - ATPases ) are large , multimeric
proton pumps that , like the related family of
F1F0 ATP synthases , employ a rotary mechanism . -
pubmed
ATP hydrolysis by the peripheral
V1 domain drives rotation of a rotary complex ( the rotor ) relative to the stationary part of the enzyme ( the stator ) , leading to proton translocation through the integral V0 domain . -
pubmed
Unlike the corresponding domains in
F1F0 , the dissociated
V1 domain does not hydrolyze
ATP , and the free V0 domain does not passively conduct protons . -
pubmed
275 , 21761 - 21767 ) showed that
subunit H ( part of the stator ) inhibits
ATP hydrolysis by free
V1 . -
pubmed
To test the hypothesis that
subunit H accomplishes this by bridging rotor and stator in free
V1 ,
cysteine - mediated cross - linking studies were performed . -
pubmed
Our experiments suggest that activation
Insig-1 by drugs leads to reduced levels of active
Srebp-1 and consequently to reduced target gene expression including the genes responsible for
triglyceride synthesis . -
pubmed
In addition , observed that
Insig-1 is also a target of
AMP - activated kinase , the hepatic activity of which is increased by activators of
CAR and
PXR and is known to cause a reduction of
triglycerides . -
pubmed
The analyte was isocratically eluted on a Venusil
XBP C8 column ( 200 mm x 3.9 mm ID , 5 microm ) with
methanol -
water mol x L(-1 )
ammonium formate ) ( 80:20 ) at a flow rate of 0.4 mL x min(-1 ) , and detected ( containing 5 mmol x L(-1 )
ammonium formate ) ( 80:20 ) at a flow rate of 0.4 mL x min(-1 ) , and detected with a triple quad LC -
MS /
MS using ESI with positive ionization . -
pubmed
Among the discordant cases , MRI was superior in demonstrating
tumor involvement in the parapharyngeal space , skull base , intracranial area , sphenoid
sinus , and retropharyngeal nodes while PET /
CT was superior in demonstrating neck
nodal metastasis . -
pubmed
Folate binds to
dihydrofolate reductase (
DHFR ) to form a binary complex whose structure maintains the overall configuration of the
enzyme ; however , some significant changes are evident when a comparison is made to the
enzyme . -
pubmed
Differential
estradiol and selective
estrogen receptor modulator ( SERM ) regulation of
Keratin 13 gene expression and its underlying mechanism in
breast cancer cells . -
pubmed
Expression of the
Keratin 13 (
KRT13 ) gene , which encodes a cytoskeletal protein thought to play important roles in
breast cancer growth and metastasis , is differentially regulated by
estradiol (
E2 ) and the selective
estrogen receptor modulators ( SERMs )
tamoxifen and
raloxifene . -
pubmed
While stimulation of
KRT13 by
tamoxifen is robust and prolonged , stimulation by
E2 is more transient and
raloxifene has virtually no effect . -
pubmed
Directed composite mutations in this region revealed that three
estrogen response elements and three
Sp1 sites were involved in its ligand - dependent regulation . -
pubmed
Differential recruitment of
ERalpha and cofactors to the
KRT13 regulatory sites paralleled the different time course and magnitude of regulation by these ligands : there was almost no
ERalpha or cofactor recruitment with
raloxifene , whereas there was strong , prolonged
ER recruitment and histone acetylation with
tamoxifen , and an early and more transient recruitment with
E2 . -
pubmed
Taken together , our results suggest that the different ligand regulations of
KRT13 are due to ligand - differential recruitment of
ER and coactivators , and they provide insight into the mechanisms responsible for the different agonistic activities and differential gene regulation by
estradiol and the SERMs
tamoxifen and
raloxifene . -
pubmed
The
CPM loaded in the resinates ( drug - loaded resins ) increased with the increasing degree of
sulfonic group and hence the drug binding site in the employed resins . -
pubmed
The
CPM release was lower from the resins with the higher degree of
sulfonic group due to the increase in the diffusive path depth . -
pubmed
The
CPM release was obviously lower in SGF than SIF because
CPM , a weak base drug , ionized to a greater extent in SGF and then preferred binding with rather than releasing from the resins . -
pubmed
The open reading frame ( ORF ) of 297 nucleotides ( nt ) of
Lycstefin encodes a protein of 99
amino acids ( aa ) with a putative molecular weight of 11kDa , in which no signal peptide and potential N - glycoslation site are predicted . -
pubmed
Recombinant
Lycstefin protein fused with
glutathione S - transferase (
rLycstefin ) was shown to have strong inhibitory activity against
papain with a K(i ) of 1.3x10(-13)M. The in vivo experiments revealed that
Lycstefin could not modulate the expression levels of large yellow croaker
tumor necrosis factor - alpha2 (
TNF - alpha2 ) and
interleukin-10 in spleen and head kidney . -
pubmed
CdS magic - sized nanocrystals exhibiting
bright band gap photoemission via thermodynamically driven formation . -
pubmed
CdS magic - sized nanocrystals ( MSNs ) exhibiting both band gap absorption and emission at 378 nm with a narrow bandwidth of approximately 9 nm and quantum yield ( QY ) of approximately 10 % ( total QY approximately 28 % , in
hexane ) were synthesized via a one -
pot noninjection approach . -
pubmed
This
CdS MSN ensemble is termed as Family 378 . -
pubmed
( 13 ) C solid - state cross - polarization magic - angle spinning (
CP / MAS ) nuclear magnetic resonance ( NMR ) demonstrates that the capping ligands are firmly attached to the nanocrystal surface via
carboxylate groups . -
pubmed
Observation of terahertz vibrations in the
nitrogenase FeMo cofactor by femtosecond pump - probe spectroscopy . -
pubmed
In addition ,
CD14(high ) bone marrow monocytes showed relatively high levels of uptake of fluorescently labeled
risedronate , which correlated with selective accumulation of unprenylated
Rap1A in
CD14(+ ) cells , as well as osteoclasts , following treatment with
risedronate in vivo . -
pubmed
The degradation was found to be enzymatic and dependent on a cofactor ,
UDP -
glucose , suggesting that
UDP - glucosyltransferase or other
UDP - glucose - dependent enzymes were involved . -
pubmed
AIM : To estimate the frequency of
relapses of
thrombotic and
hemorrhagic complications during moderately intensive therapy for
antiphospholipid syndrome (
APS ) with
warfarin with and without
aspirin . -
pubmed
, at 1 , 12 and 24h ) , ( 2 ) effects of single ( 1h ) or multiple ( 1 , 12 and 24h )
ethyl pyruvate treatments (
EP ; at 20 or 40 mg / kg , i.p . ) -
pubmed
Pyruvate treatment(s ) attenuated CCI - induced reductions of cerebral
cytochrome oxidase activity at 7 2h , significantly improving activity in peri - contusional cortex after multiple SP or
EP treatments . -
pubmed
Ipsilateral CA3(b )
neuronal loss , but not cortical tissue loss , was significantly reduced 1 month
post - CCI with
pyruvate treatments begun 1h
post - CCI . -
pubmed
While water and
electrolytes can freely pass through the glycocalyx , plasma proteins , especially
albumin , interact strongly . -
pubmed
RESULTS : The radiochemical purity (
RCP ) of the
( 99m ) Tc - BnAO - NI preparation was greater than 96 % and stable at ambient temperature for 24h ( RCP>90 % ) . -
pubmed
The
tumor - to - muscle ratios at 2h
post - injection of
( 99m ) Tc - BnAO - NI with and without
pentoxifylline pretreatment were 1.67 + - 0.38 and 2.59 + - 0.25 , respectively ( p = 0.025 , n = 3 ) . -
pubmed
We hypothesized that application of the
TLR4 antagonist
eritoran may also reduce
cardiac hypertrophy after
TAC surgery . -
pubmed
In contrast ,
IL-10 was significantly increased in both
eritoran groups independent from
TAC . -
pubmed
CONCLUSION : Application of the
TLR4 antagonist
eritoran attenuates the development of
cardiac hypertrophy possibly by a reduction in inflammatory and increase in anti - inflammatory
cytokines . -
pubmed
There are some intriguing results and hypotheses about the link between
isoprenoid metabolism and the
IL-1 pathway through geranylgeranylation that deserve to be further examined . -
pubmed
Treatment with the
HDAC inhibitor
trichostatin A increased the incidence of an abnormal metaphase but had no adverse effect on maturation efficiency . -
pubmed
The resulting materials were thoroughly characterized by CHS elemental analysis , X - ray diffraction , transmission electron microscopy ,
N2 physisorption and
H2 chemisorption , as well as by Raman , NMR , FTIR and
EPR spectroscopies , in order to investigate the influence of the temperature and atmosphere of the pretreatment on the gold particle size and oxidation state , the formation of defective E ' centres on the
silica support and their ability to induce the formation of reactive
O2 (- ) surface species . -
pubmed
Epitaxial surface growth of
oligophenylenes on a heated
muscovite mica crystal results in a thin film of mutually aligned nanofibres with dimensions of tens of nanometres in height , hundreds of nanometres in width and tens to hundreds of micrometres in length . -
pubmed
Here we used a new
MS screening approach to reveal that
Ybt is also a physiologically favorable
Cu ( II ) ligand . -
pubmed
Chemical and genetic characterization showed that
Ybt helps bacteria resist
copper toxicity by sequestering host - derived
Cu ( II ) and preventing its
catechol - mediated reduction to
Cu ( I ) . -
pubmed
Together , these studies reveal a new virulence - associated function for
Ybt that is distinct from
iron binding . -
pubmed
This study presents a systematic detailed experimental and theoretical investigation of the electronic properties of size - controlled free and gamma -
Al ( 2 ) O ( 3 )-supported
Pt nanoparticles ( NPs ) and their evolution with decreasing
NP size and adsorbate (
H ( 2 ) ) coverage . -
pubmed
A correlation between the
NP size , number of surface atoms and coordination of such atoms , and the maximum
hydrogen coverage stabilized at a given temperature is established , with H /
Pt ratios exceeding the 1 : 1 ratio previously reported for bulk
Pt surfaces . -
pubmed
In particular ,
JVH1 is able to selectively cleave
carbon -
sulfur bonds within
alkyl chains . -
pubmed
The variability of peak current of
L - type calcium channel ( I(
Ca , L ) ) shows an increase in cardiomyocytes after 6 h of preservation when the acutely isolated cardiomyocytes are preserved in a small volume buffer solution . -
pubmed
In order to get stable peak I(
Ca , L ) , we need to not only select a normal shape of cardiomyocyte at a
bright field but also a blue fluorescent myocyte at an ultraviolet excitation . -
pubmed
Presence of
catalase or
indomethacin or
SQ 29,548 reduced significantly the pressure - induced myogenic response . -
pubmed
Here we verify whether these changes are associated with the occurrence of
acute phase response (
APR ) to intravenous (
IV )
zoledronic acid (
ZOL ) or changes of other circulating white blood cells ( WBC ) . -
pubmed
Influence of mild and moderate
liver impairment on the pharmacokinetics and metabolism of
almorexant , a dual
orexin receptor antagonist . -
pubmed
This single - dose study aimed at investigating the effect of different degrees of
hepatic impairment on the pharmacokinetics ( PK ) , metabolism , and tolerability of
almorexant , a first - in - class dual
orexin receptor antagonist . -
pubmed
Twenty - seven
VvAP genes were expressed in at least one of the six tissues examined ; nineteen
VvAPs responded to at least one abiotic stress , 12
VvAPs responded to
powdery mildew infection , and most of the
VvAPs responded to
SA and
ABA treatments . -
pubmed
The crystal structure of
Rv3717 in complex with a
dipeptide product shows that , compared with previously characterized
peptidoglycan amidases , the enzyme contains an extra
disulfide - bonded beta - hairpin adjacent to the active site . -
pubmed
DESIGN : Fibroblasts derived from
keloid tissue were incorporated in either Matrigel or
polyethylene glycol diacrylate mixed with
type I collagen to create 3 - dimensional models to investigate the role
MMPs play in
keloid formation . -
pubmed
The dying cells exhibit
FITC -
annexin V staining , indicative of programmed cell
death ( apoptosis ) . -
pubmed
In all 4 ,
ACTH - stimulated
aldosterone concentrations were below the detection limit of the assay . -
pubmed
Aldosterone concentrations were not different at 30 , 45 , or 60 minutes after
ACTH administration . -
pubmed
In this study , an electrochemical method , the cyclic voltammetry method , basing on loop - mediated isothermal amplification (
LAMP ) , electrochemical ion bonding ( positive dye ,
methylene blue ) , was introduced . -
pubmed
The serum
adiponectin levels were higher in the healthy group and showed a positive correlation with
HDL cholesterol levels in the unhealthy group . -
pubmed
According to the number of
cysteines in their active site ,
Grx are classified as
dithiolic or monothiolic ( 1 - C - Grx ) . -
pubmed
Inositol 1,4,5 - trisphosphate (
IP3 ) is a universal signalling molecule that releases
calcium from stores within cells by activating the
IP3 receptor . -
pubmed
A novel sample preparation method to probe the solution phase structure of dimerized
Gramicidin A (
GA ) inserted into lipid vesicle bilayers is described . -
pubmed
Solution - phase physicochemical interactions of
GA influenced by lipid
acyl chain length and extent of
acyl chain unsaturation can now be probed by monitoring the conformer preferences using IM -
MS . -
pubmed
Increasing the
acyl chain length from 12 to 22
carbons yields
[ 2GA + 2Na ]( 2 + ) IM -
MS profiles with reduced conformer microheterogeneity . -
pubmed
Superoxide dismutase activity and
malondialdehyde content responded efficiently to in situ contamination when a certain degree of contamination was reached . -
pubmed
We developed and studied hybrid bilayer
wound dressings which combine a drug - loaded porous
poly ( dl - lactic - co - glycolic acid ) top layer with a spongy
collagen sublayer . -
pubmed
Superoxide dismutase (
SOD ) and
catalase (
CAT ) activities , along with
malondialdehyde (
MDA ) level were determined in skin tissue homogenates of the dermal
wounds . -
pubmed
macrocarpa fruit extract increased the
SOD and
CAT activities in the healing
wounds , thereby significantly increasing
MDA level . -
pubmed
Vitamin D Receptor Gene
FOKI Polymorphism Contributes to Increasing the Risk of HIV - Negative
Tuberculosis : Evidence from a Meta - Analysis . -
pubmed
BACKGROUND :
Vitamin D receptor (
VDR ) gene
FokI polymorphism have been studied in relation to
tuberculosis (
TB ) in many populations and provided inconsistent results . -
pubmed
On the first
CT reevaluation , the
sorafenib response rates were 20 % , 5 % , 7 % and 3 % according to Choi , EASL , mRECIST and RECIST 1.1 . -
pubmed
In
keratitis - ichthyosis - deafness syndrome , different
connexin 26 mutations can either form dominant hemichannels with altered
calcium regulation or increased
calcium permeability , leading to clinical subtypes of this
syndrome . -
pubmed
Polyriboinosinic - polyribocytidylic acid ( polyI : C ) , a ligand for
toll - like receptor 3 , was selected as a model nucleic acid - based adjuvant . -
pubmed
The in vitro degradation behaviors of the bare
AZ31 alloy and coated samples were evaluated in a simulated body fluid (
SBF ) using the potentiodynamic polarization curve test and the static immersion test . -
pubmed
The in vitro degradation tests showed that the
PEO /
PCL composite coating improved the corrosion resistance of the
AZ31 alloy in
SBF with a more positive corrosion potential and a lower corrosion current density . -
pubmed
After further surface functionalization by
polydopamine , the corrosion resistance of the composite coating was maintained , while its bioactivity was significantly enhanced with a large amount of
hydroxyapatite ( HA ) formed on its surface after immersion in
SBF . -
pubmed
This feasible method for fabricating a cytocompatible and antibacterial composite coating on a biodegradable
AZ31 alloy may be promising in implant applications due to the osteointegration and anti -
infection properties of these materials
post operation . -
pubmed
Lipin-1 exhibits a dual function : an enzyme that catalyzes the conversion of
phosphatidate to
diacylglycerol and a transcriptional regulator . -
pubmed
Our data showed that
resveratrol significantly increased the levels of
Lipin-1 protein and mRNA in HSCs . -
pubmed
Further investigation revealed that
resveratrol blocked the polyubiquitination of
Lipin-1 . -
pubmed
Resveratrol inhibited
TGF - beta - induced fibrogenic gene expression . -
pubmed
TGF - beta - induced SMAD binding element -
luciferase reporter activity was significantly diminished by
resveratrol with a simultaneous decrease in
SMAD3 phosphorylation . -
pubmed
Consistently , knockdown of the
Lipin-1 gene using siRNA abolished the inhibitory effect of
resveratrol . -
pubmed
We conclude that
Lipin-1 can antagonize HSC activation through the inhibition of
TGF - beta /
SMAD signaling and that
resveratrol may affect
Lipin-1 gene induction and contribute to the inhibition of
TGF - beta - mediated hepatic fibrogenesis . -
pubmed
The most common grade 3 - 4 adverse events in the
leucovorin and
fluorouracil group were
neutropenia without
fever ( 17 [ 23 % ] ) ,
fatigue ( 16 [ 22 % ] ) ,
paraesthesia ( 14 [ 19 % ] ) ,
diarrhoea ( nine [ 12 % ] ) , and
mucositis ( seven [ 10 % ] ) ; in the
gemcitabine group they were
neutropenia without
fever ( 12 [ 32 % ] ) ,
thrombocytopenia ( seven [ 18 % ] ) ,
fatigue ( eight [ 21 % ] ) ,
anaemia ( five [ 13 % ] ) , increased
alanine aminotransferase and
aspartate aminotransferase concentrations ( five [ 13 % ] for both ) , and
paraesthesia ( four [ 11 % ] ) . -
pubmed
Grade 1 - 3 elevation of
bilirubin ,
alkaline phosphatase ,
aspartate aminotransferase , and
alanine transaminase were more frequently recorded in arm A than in arm B. -
pubmed
METHODS : PM10 concentrations were recorded and filter paper samples were collected using E - samplers ;
PM1 , PM2.5 , black
carbon ( BC ) ,
methane (
CH4 ) , and
carbon monoxide (
CO ) were also measured . -
pubmed
We analysed NOx (
nitrite+
nitrate ) and
hydrogen peroxide (
H2O2 ) concentrations , transcript levels of
endothelial nitric oxide synthase (
eNOS)/
inducible nitric oxide synthase (
iNOS ) and
arginine modulators ,
hydrogen peroxide regulators in the cohort . -
pubmed
The transcripts involved in
arginine bioavailability :
Argininosuccinate lyase (
ASL ) ,
Solute Carrier Family1 , member 7 (
SLC7A1 ) and
Arginase 1 (
ARG1 ) and Asymmetric
Dimethyl Arginine (
ADMA ) metabolism :
Protein arginine methyltransferase 1 (
PRMT1 ) and
Dimethylarginine dimethylaminohydrolase 2 (
DDAH2 ) also showed differential expression . -
pubmed
Biomarkers of kidney function were determined , including
glomerular filtration rate ( eGFR ) estimated from the
Chronic Kidney Disease Epidemiology Collaboration equation , urine
albumin - to -
creatinine ratio (
ACR ) ,
microalbuminuria status , and glomerular hyperfiltration status . -
pubmed
All the processes are done in a single system ( one -
pot and in - situ processing ) based on deep eutectic
choline chloride -
calcium chloride (
Calcoline ) at mild temperature of 50 C in direct contact with air . -
pubmed
About 70 % of the adult world population is
lactose - intolerant , due to low levels of intestinal
lactase , also called
lactase - phlorizin hydrolase (
LPH ) , a
beta - d - galactosidase found in the apical surface of the intestinal microvilli . -
pubmed
The photocatalytic degradation rates of NCTs were identical with respect to their adsorption capacities , and the apparent rate constant ( kapp ) values indicated that the amount of
TiO2 in NCTs played a vital role in the degradation of
MEF . -
pubmed
,
Ac - MtFluNox ,
Lyso - RhoNox , and
ER -
SiRhoNox , which can detect
Fe ( ii ) specifically in the mitochondria , lysosomes , and endoplasmic reticulum (
ER ) , respectively . -
pubmed
The present article reviews various attempts to test drugs approved for different purposes such as
calcium channels blockers , non - steroidal anti - inflammatory drugs ,
cardenolides , antidiabetic , antibiotics and antimalarial for treating
retinoblastoma . -
pubmed
METHODS : Disc - shaped specimens ( n=15 ; O=10 mm ; thickness=1.0 mm ) were produced from
CAD / CAM blocks as follows : feldspathic ( FEL ) ;
leucite ( LEU ) ;
lithium disilicate (
LD ) ;
zirconia - reinforced
lithium silicate ( ZRLS ) ; polymer - infiltrated ceramic network ( PICN ) ; and resin nanoceramic ( RNC ) . -
pubmed
Hypermethylation of
transcription factor activating enhancer - binding protein 2e (
TFAP2E ) has been reported to be associated with chemoresistance to
5 - fluorouracil (
5 - FU ) in
gastric cancer (
GC ) . -
pubmed
Collectively , our data support an important role of exosomes and exosomal miRNAs in
TFAP2E methylation - induced chemoresistance to
5 - FU in
GC . -
pubmed
Measurement of ultra - trace level of intact
oxytocin in plasma using SALLE combined with nano - LC -
MS . -
pubmed
In these experiments , spinal nerve ligation (
SNL ) and a daily injection of
propentofylline ( 1 mg / kg , ip ) as a glial cell inhibitor agent , 1 h following nerve ligation during 7 - day
post -
SNL period , were performed . -
pubmed
Prednisolone treatment induced temporary
factor IX normalization in mild
hemophilia B who required an epidural infiltration : A case report . -
pubmed
Surprisingly , hemostasis result tests performed before epidural infiltration were a normal activated partial
thrombin time at 36.1 s. ( normal range 27.9 - 37.7 s. ) and
factor IX (
FIX ) level 76 % ( normal range>70 % ) , 13 days after
prednisolone introduction . -
pubmed
By using both in vivo and ex vivo approaches , we have found that pharmacological doses of
progesterone ( P4 ) and
estradiol (
E2 ) produced an inhibition in the expression of hypothalamic
GnRH , while physiological doses produced a differential effect on the pulsatile release frequency or genomic expression of
GnRH . -
pubmed
Our ex vivo experiment indicates that a short - term effect of
E2 modulates the frequency of
GnRH release pattern that would be associated with membrane
ERs . -
pubmed
On the other hand , our in vivo approach suggests that a long - term effect of
E2 , acting through the classical nuclear
ERs -
PRs pathway , would produce the modification of
GnRH mRNA expression during the
GnRH pre - ovulatory surge . -
pubmed
This paper describes the fabrication of an imprinted fluorescent nanoprobe based on
SiO2 - coated
NaYF4 :
Yb ,
Er upconversion nanoparticles ( UCNP ) encapsulated with a molecularly imprinted polymer (
MIP ) for determination of
acetamiprid . -
pubmed
The fluorescent
MIP nanoprobe was prepared using UCNP as the material for fluorescence signal readout ,
acetamiprid as template molecule ,
methylacrylic acid (
MAA ) as functional monomer , and
ethyleneglycol dimethacrylate (
EGDMA ) as cross - linking agent . -
pubmed
UCNP@MIP shows a high selectivity towards
acetamiprid with an imprinting factor (
IF ) of 7.84 . -
pubmed
Graphical abstractA simple fluorescence nanoprobe which integrates upconversion nanoparticles ( UCNPs ) and molecular imprinting polymer (
MIP ) was developed for the determination of
acetamiprid . -
pubmed
Gene panel test showed c.697A > G heterozygous variant in the
PSEN1 gene leading to a M233V
amino acid change , which was validated by a Sanger sequencing . -
pubmed
In published O - glycoproteomics data , our method more than doubled the number of
glycoPSMs annotated when searching the same
glycans as the original search , and yielded 4- to 6 - fold increases when expanding searches to include additional
glycan compositions and other modifications . -
pubmed
In this paper , we report on our simple innovative approach in the selection of best - transfected cells with the highest expression of
ASPH using subclone selection , quantitative real - time
polymerase chain reaction , and gradual increment of
hygromycin concentration . -
pubmed
Furthermore , the gradual increment of
hygromycin ( up to 400mg / mL ) improved the expression of
ASPH . -
pubmed
Succinic acid inhibits the activity of
cytochrome P450 (
CYP450 )
enzymes . -
pubmed
OBJECTIVE : The effect of
succinic acid on the activity of
cytochrome P450 (
CYP450 )
enzymes was investigated in this study . -
pubmed
MATERIALS AND METHODS : The effect of
succinic acid ( 100 muM ) on the activity of eight isoforms of
CYP450 ( i.e. -
pubmed
RESULTS :
Succinic acid exerted inhibitory effect on the activity of
CYP3A4 ,
2D6 , and
2C9 with the IC50 values of 12.82 , 14.53 , and 19.60 muM , respectively . -
pubmed
Succinic acid inhibited the activity of
CYP3A4 in a non - competitive manner with the Ki value of 6.18 muM , and inhibited
CYP2D6 and
2C9 competitively with Ki values of 7.40 and 9.48 muM , respectively . -
pubmed
DISCUSSION AND CONCLUSIONS :
Succinic acid showed in vitro inhibitory effects on the activity of
CYP3A4 ,
2D6 , and
2C9 , which indicated the potential drug - drug interactions . -
pubmed
Succinic acid should be carefully co - administrated with the drugs metabolized by
CYP3A4 ,
2D6 , and
2C9 . -
pubmed
This paper demonstrates that the molecular conformation ( in addition to the composition and structure ) of molecules making up self - assembled monolayers ( SAMs ) influences the rates of charge tunneling (
CT ) through them , in molecular junctions of the form
AuTS /
S ( CH2 ) 2CONR1R2//
Ga2O3 /
EGaIn , where
R1 and
R2 are
alkyl chains of different length . -
pubmed
tunneling barrier width ) and their rectification ratios at + -1.0 V. When
R1 =
H , the molecules are well ordered and exist predominantly in trans - extended conformations . -
pubmed
As shown previously for heterogeneous nuclear RNA (
HnRNA ) and 45 S precursor ribosomal RNA ( pre - rRNA ) ,
proflavine immediately stabilizes newly synthesized adenovirus RNA , while
ethidium bromide stabilizes the RNA after a 30 - -60 min lag period . -
pubmed
Partial isolation and translation in vitro of messenger ribonucleic acid for
phosphoenolpyruvate carboxykinase (
guanosine triphosphate ) . -
pubmed
The presence of newly synthesized
phosphoenolpyruvate carboxykinase (
GTP ) was detected by immunoprecipitation with a specific antibody to the enzyme and analysis by
sodium dodecyl sulphate /
polyacrylamide - gel electrophoresis . -
pubmed
The optimal concentrations of
magnesium acetate and
KCl for
phosphoenolpyruvate carboxykinase synthesis were determined . -
pubmed
When polyribosomal RNA was separated by
sucrose - gradient centrifugation ,
phosphoenolpyruvate carboxykinase mRNA migrated between 20 and 26 S in keeping with the high mol . -
pubmed
Thymidine kinase was eluted with 0.1 mM
thymidine , 0.1 M
KC1 in 0.01 M
Tris -
HC1 , pH 7.5 . -
pubmed
The purified
enzyme accounted for about 26 % of the applied activity ; the specific activity of the purified material ( peak fraction ) was 3,500 moles
TMP formed per mg
protein per 10 min . -
pubmed
There was , however , no correlation between the
PRA levels and the blood pressure decrease after salt depletion or after
propranolol . -
pubmed
Proceedings : Decrease in the accuracy of
DNA polymerase following treatment with gamma - rays and
methylnitrosourea . -
pubmed
The results suggest that suppression of basal
DA release in the
NAC is an adaptive consequence of sustained
cocaine exposure and may in part underlie the
post -
cocaine anhedonia observed in behavioral models of
cocaine withdrawal . -
pubmed
Essential
amino acids like
methionine ,
cystine ,
threonine and
valine were found to be limiting in the seed
proteins of both the germplasms . -
pubmed
Antinutritional substances like total free
phenols ,
tannins ,
L - DOPA ,
trypsin inhibitor activity and phytohaemagglutinating activity also were investigated . -
pubmed
In the presence of
Mo , PWM stimulation of T lymphocytes ( highly depleted of B lymphocytes ) induces as much IL-2 mRNA as
phytohemagglutinin (
PHA ) , but results in higher and persistent
IL-2 levels in culture supernatants despite the concomitant T cell mitogenesis , suggesting that PWM - activated T cells do not utilize the
IL-2 they produce . -
pubmed
Moreover , the PWM - induced T cell mitogenesis was not inhibited by
anti - IL-2 or CD25 antibodies and only partially ( 50%-60 % ) inhibited by
cyclosporin A , while these treatments abrogated the PHA - induced one . -
pubmed
In addition ,
peptide YY ( 1 mumol / L ) reduced by 42 % + /- 6 % electrically stimulated muscle contractions that were blocked by
atropine and
tetrodotoxin , providing additional evidence that the peptide inhibits release of
acetylcholine . -
pubmed
Next , the effect of
peptide YY on
potassium - evoked release of
[ 3H ] acetylcholine and whether the peptide inhibits
cyclic adenosine monophosphate - dependent release of
acetylcholine were examined . -
pubmed
In addition ,
peptide YY reduced half - maximal
forskolin and
cholera toxin - evoked release of
acetylcholine by 45 % + /- 6 % and 42 % + /- 8 % , respectively , suggesting that the peptide can inhibit
cyclic adenosine monophosphate - dependent release of
acetylcholine . -
pubmed
In addition ,
peptide YY antagonized
cyclic adenosine monophosphate - mediated release of
acetylcholine through a
pertussis toxin - sensitive mechanism . -
pubmed
During continuous exposure to
TEA , a significant increase in
PRL secretion occurred by 20 min and the response was sustained until the end of a 60 - min exposure . -
pubmed
Blocking
Ca2 + influx by employing a
Ca ( 2 +)-depleted medium or the Ca2 +
channel blocker ,
nifedipine , prevented induction of
PRL secretion by 20 mM
TEA . -
pubmed
Preincubation of the cells for 10 min with 20 mM
TEA did not inhibit
PRL secretion induced by
thyrotropin - releasing hormone (
TRH ) ,
phorbol 12 - myristate 13 - acetate (
TPA ) or by cell swelling produced by 30 % medium hyposmolarity , but significantly depressed that induced by depolarizing 30 mM
K + . -
pubmed
BaCl2 , another
K + channel blocker , had the same effect on
PRL secretion as
TEA . -
pubmed
Molecular basis of
androgen resistance in a family with a qualitative abnormality of the
androgen receptor and responsive to high - dose
androgen therapy . -
pubmed
The
androgen receptor gene in this family contains two structural alterations : a single nucleotide substitution at position 2444 in exon 5 ( adenosine----guanosine ) that converts
tyrosine 761 to a
cysteine residue and a shortened glutamine homopolymeric segment in exon 1 that encodes 12 rather than the usual 20 - 22
glutamines . -
pubmed
A family study was performed using
polymerase chain reaction amplification of the
glutamine - rich segment , and it was shown that the sister of the proband does not carry the mutant allele . -
pubmed
Marked thermolability of the transfected receptor protein , however , was demonstrable only upon introduction of an
androgen receptor cDNA containing both the partial deletion of the
glutamine homopolymeric segment and a
cysteine residue at position 761 . -
pubmed
Specific stages of the prereplicative
G1 phase of the cell cycle in nonneoplastic cells requires extracellular
Ca2 + for successful transition . -
pubmed
One of these agonist ,
inositol 1,3,4,5 - tetrakisphosphate [
Ins ( 1,3,4,5 ) P4 ] , has been demonstrated by us to also stimulate the activity of a
phosphoprotein phosphatase . -
pubmed
A patch -
clamp study of excitatory
amino acid activated channels . -
pubmed
In contrast ,
dexamethasone was found to inhibit the induction of transin mRNA when added with , or following ,
NGF treatment . -
pubmed
Finally , we show that sequences contained within 750 bp of the 5 ' untranscribed region of the
transin gene confer responsiveness to
NGF and
dexamethasone . -
pubmed
The upper 95th percentile for the difference between two yearly measurements , expressed as a percent of the population mean , ranged from 4 % for
Na + to approximately 20 % for total
cholesterol and to greater than 90 % for
ferritin . -
pubmed
Year - to - year differences attributable to the biological component ranged from a low of 2 % of the population mean for
Na+ to 70 % for
gamma - glutamyltransferase . -
pubmed
A different hydrosmotic response for
oxytocin and
isoproterenol ( but not for A
II ) was found between four cutaneous regions of toad body . -
pubmed
The configuration of
M13 bacteriophage coat protein in a model membrane was analyzed using
protease digestion followed by gel permeation chromatography on Fractogel TSK in
formic acid /
ethanol . -
pubmed
A new reconstitution was thereby developed which involves adding a small volume of a concentrated solution of
cholate - solubilized
coat protein to preformed vesicles ( with the amount of detergent added being less than that required to solubilize the vesicles ) , freezing in liquid
nitrogen , thawing , followed by dialysis to remove excess detergent . -
pubmed
Cleavage of
transferrin by
cyanogen bromide . -
pubmed
We show that : ( 1 )
Epinephrine in the 10(-7)-10(-3 ) M concentration range ( ED50 = 11.10(-6 ) M ) inhibits
VIP - induced
cyclic AMP rise in isolated colonic epithelial cells ; the maximal inhibition reaches 30 % of
VIP effect ;
epinephrine alters the efficacy of the peptide and does not modify its potency ;
epinephrine also reduces the basal
cyclic AMP level . -
pubmed
( 3 ) The inhibition of
VIP action by
epinephrine is reversed by the alpha antagonists
dihydroergotamine ,
phentolamine and the alpha 2 antagonist
yohimbine , while unaffected by the beta antagonist
propranolol and the alpha 1 antagonist
prazosin , ( 4 )
Epinephrine inhibits
VIP - stimulated
adenylate cyclase activity in preparations of colonic plasma membranes . -
pubmed
After immunization with the primary test antigen
haemocyanin , a decreased antigen specific humoral immune response was observed for all three
Ig classes , irrespective of disease activity or the use of
corticosteroids . -
pubmed
Capsaicin and
substance P produced similar excitatory effects in both quiescent and rhythmically contracting bladders , i.e. -
pubmed
Atropine pretreatment prevented the neurogenic component of the excitatory effect of both
capsaicin and substance P. Repeated applications of
capsaicin but not of
substance P led to desensitization . -
pubmed
Experiments have been carried out in the presence of
indomethacin (
IN ) , specific inhibitor of
prostaglandin - synthetase . -
pubmed
Lower temperature and
orthophosphate control of
alkaline phosphatase in a cold - requiring plant . -
pubmed
The elevated activity of
alkaline phosphatase observed in roots and shoots of Verbascum rosettes grown in vitro at 15 degrees C may be the result of a reduced supply of
orthophosphate . -
pubmed
The rosette reveals greater activities of this
enzyme under conditions of lower external
phosphate or chilling temperature . -
pubmed
The stimulation of
phosphatase in tissues having lower levels of
phosphate is thus a possible feature of other plant species which also display freezing tolerance . -
pubmed
There were three major findings in the various combinations of
CBD , HND , and
SA in ponies during treadmill exercise . -
pubmed
First , the combination of
CBD with HND or
SA resulted generally in an accentuation of the
hypocapnia during exercise that was predictable on the basis of
CBD alone . -
pubmed
However , in one pony that showed a
hypercapnic exercise response after
SA alone ,
CBD subsequently caused a greater exercise
hypercapnia . -
pubmed
O2 binding properties of the separated aggregating and non - aggregating
Hbs purified at low pH can not account for the functional properties of the composite hemolysate , suggesting the presence of other subunits interactions . -
pubmed
glutinosa
Hb , where the presence of three major monomeric
Hbs and a possible
CO2 - dependent aggregation had been reported . -
pubmed
At the end of reperfusion , bile production ,
lactate dehydrogenase (
LDH ) secretion , and tissue
adenosine triphosphate (
ATP ) was measured . -
pubmed
Viability of the group B livers , assessed by bile production , tissue
ATP value , and
LDH release , was preserved better than that of group A livers . -
pubmed
The phenotype of
mis5 is DNA replication defect and
hypersensitivity to UV ray and
hydroxyurea . -
pubmed
Since an increase in cytoplasmic free
Ca2 + concentration ( [
Ca2+]i ) in the pancreatic beta cell is the major stimulus for
insulin release , this study was undertaken to investigate the dynamics of electrical activity , [
Ca2 +]i - changes and
insulin release , in stimulated islets from subjects of varying
glucose tolerance . -
pubmed
Antidepressant / anxiolytic
ipsapirone inhibits cold - induced hypothalamic
TRH release . -
pubmed
Acute administration of the
serotonin ( 5 - HT ) 1A receptor agonist
ipsapirone has been previously shown to elicit a dose - dependent decrease of cold - elicited
thyrotropin (
TSH ) secretion . -
pubmed
Therefore , the aim of the present study was to evaluate , by means of a push - pull cannula implanted in the median eminence ( ME ) , whether
ipsapirone acts primarily through an inhibition of cold - elicited
thyrotropin - releasing hormone (
TRH ) release . -
pubmed
1 - Aminocyclopentane - trans-1S,3R - dicarboxylic acid (
1S,3R - ACPD ) , a compound which selectively activates a
metabotropic glutamate receptor (
mGluR ) , suppresses hippocampal excitatory synaptic transmission . -
pubmed
This
depression is not antagonizable by
L-2 - amino-3 - phosphonoproprionic acid (
L - AP3 ) , an agent which counteracts
mGluR - induced
phosphoinositide (
PI ) metabolism in several neuronal systems . -
pubmed
Therefore , we believe that
1S,3R - ACPD activates a novel hippocampal
mGluR subtype that is either insensitive to
L - AP3 blockade and/or linked to an effector system that does not involve
PI turnover . -
pubmed
Recently ,
hormone receptor status (
estrogen [
ER ] ,
progesterone [ PR ] ) in
endometrial cancer has been suggested to be a prognostic factor . -
pubmed
Release of tritiated water into the medium was thereupon detected utilizing its lack of binding to active
charcoal , which is an established technique to measure in situ
thymidylate synthase activity . -
pubmed
Like water ,
dihydrouracil does not bind to active
charcoal , and its presence in the cell medium can result in an overestimation of the in situ
thymidylate synthase activity . -
pubmed
The catabolism of
dCyd was highest in macrophages where 25 % of the added
dCyd ( 0.5 microM , 0.5 nmol / million cells ) had been converted to
dihydrouracil after 30 min , and 90 % after 12 h. The in situ
thymidylate synthase activity was found to be the highest in stimulated lymphocytes . -
pubmed
This study investigated whether the circadian regulation of
luteinizing hormone (
LH ) release may be through direct input of the suprachiasmatic nucleus (
SCN ) to
estrogen receptor (
ER)- and/or gonadotropin releasing hormone (
GnRH)-immunoreactive neurons . -
pubmed
Results suggest that the circadian system could regulate the timing of the
LH surge via two pathways , through input to
GnRH and to
ER cells . -
pubmed
Overexpression of
LAS1 results in extra cell surface projections in the mother cell , alterations in
actin and
SPA2 localization , and the accumulation of
electron - dense structures along the periphery of both the mother cell and the bud . -
pubmed
Effect of a novel
5 - hydroxytryptamine3 ( 5 - HT3 ) receptor antagonist ,
GK-128 , on
5 - HT3 receptors mediating contractions and relaxations in guinea - pig distal colon . -
pubmed
We investigated
5 - hydroxytryptamine3 ( 5 - HT3 ) receptor - mediating contractions and relaxations in the guinea - pig isolated distal colon using various
5 - HT3 receptor agonists and antagonists , including
GK-128 (
2 -[( 2 - methylimidazol-1 - yl ) methyl ] benzo [ f ] thiochromen-1 - one monohydrochloride hemihydrate ) . -
pubmed
Selective
5 - HT3 receptor agonists ,
2 - methyl-5 - HT and
m - chlorophenylbiguanide , produced
spantide - insensitive contraction and
atropine - insensitive contraction and the relaxation . -
pubmed
Other selective
5 - HT3 receptor antagonists ,
azasetron and
tropisetron , also exhibited higher affinity in contraction than in relaxation , but the extent of their affinity differences was smaller than that observed in
GK-128 . -
pubmed
These results suggest that the
5 - HT3 receptors which mediate contraction and relaxation in the guinea - pig distal colon may not be the same , and that
GK-128 is a
5 - HT3 receptor antagonist with a stronger potency for contraction . -
pubmed
Lower - efficacy
D1 or
D2 agonists substituted for
methamphetamine either partially (
SDZ 208 - 911 ) or not at all (
SKF 77434 ,
SDZ 208 - 912 ) . -
pubmed
Pretreatment with
dopamine receptor blockers [
D1 (
SCH 39166 , 0.1 mg / kg ) or
D2 (
remoxipride , 3.0 mg / kg and
nemonapride , 0.003 mg / kg ) ] and low - efficacy agonists [
D1 (
SKF 77434 ; 3.0 mg / kg ) or
D2 (
SDZ 208 - 911 and
SDZ 208 - 912 ; 0.01 - 0.03 mg / kg ) ] antagonized the discriminative - stimulus effects of
methamphetamine . -
pubmed
These results support the view that both dopaminergic
D1 and
D2 mechanisms mediate the discriminative - stimulus effects of
methamphetamine ; further , they indicate that selected
dopamine D1 partial agonists may have antagonist actions at doses that do not produce undesirable effects associated with
dopamine receptor blockade . -
pubmed
In this study , we conducted a nested reverse transcription -
polymerase chain reaction ( RT - PCR)-based assay to detect the
TLS /
FUS -
CHOP fusion gene transcripts using archival
formalin - fixed , paraffin - embedded
tumor specimens . -
pubmed
The present study confirms previous data about the effects of
Zn supplementation on aging processes and demonstrates that
HF - MRI is a powerful technique to study processes of aging , providing information about the effects of drug treatments on these processes . -
pubmed
Kemptide and autophosphorylation activities of
PrKX are strongly inhibited by the
RIalpha subunit and by
protein kinase inhibitor in vitro , but only weakly by the
RIIalpha subunit . -
pubmed
The inhibition by the
RIalpha subunit is reversed by addition of nanomolar concentrations of
cAMP ( Ka = 40 nM ) , thus demonstrating that
PrKX is a novel ,
type I cAMP - dependent protein kinase that is activated at lower
cAMP concentrations than the holoenzyme with the
Calpha subunit of cAMP - dependent protein kinase . -
pubmed
Microinjection data clearly indicate that the type I R subunit but not type
II binds to
PrKX in vivo , preventing the translocation of
PrKX to the nucleus in the absence of
cAMP . -
pubmed
The
RIIalpha subunit is an excellent substrate for
PrKX and is phosphorylated in vitro in a
cAMP - independent manner . -
pubmed
We discuss how
PrKX can modulate the
cAMP - mediated signal transduction pathway by preferential binding to the
RIalpha subunit and by phosphorylating the
RIIalpha subunit in the absence of
cAMP . -
pubmed
NOEs , chemical shift perturbations and temperature coefficients of
amide protons in aqueous and nonaqueous media suggest that
carbohydrate moiety in
threonine glycosylated peptides ( series
II ) is in close proximity to the peptide backbone . -
pubmed
Delineation of solution structure of these ( glyco)peptides by NMR and
CD revealed largely a
poly L - proline type II and/or random
coil conformation for the peptide core . -
pubmed
Typical peptide fragments of tandem repeat sequence of
mucin (
MUC7 ) showing profound glycosylation effects and distinct differences between
serine and
threonine glycosylation as observed in the present investigation could serve as template for further studies to understand the multifunctional role played by
mucin glycoproteins . -
pubmed
IntI1 protein , as well as
IntI1 - COOH , a truncated form containing its
carboxy - terminal domain , has been purified . -
pubmed
IntI1 -
COOH did not bind either attI or
attC , indicating that the N - terminal domain of
IntI1 was required for binding to double - stranded attI. -
pubmed
Protein
IntI1 -
COOH bound ss
attC as well as did the complete
integrase , indicating that the ability of the protein to bind ss aadA1 attC was contained in the region between
amino acids 109 and 337 of
IntI1 . -
pubmed
Binding to ss attI DNA by the
integrase , but not by
IntI1 -
COOH , was also observed and was specific for the attI bottom strand , indicating similar capabilities of
IntI1 for binding attI DNA in either double - stranded or ss conformation . -
pubmed
Insulin - sensitivity index and
carbohydrate and lipid metabolism in
gestational diabetes . -
pubmed
The
insulin - tolerance test ( ITT ) consisted of an intravenous bolus of 0.1 IU / kg of regular
insulin with
glucose sampling at -5 , 0 , 3 , 5 , 7 , 10 and 15 minutes . -
pubmed
Characterization of a
phospholipase C beta 2 - binding site near the
amino - terminal coiled -
coil of
G protein beta gamma subunits . -
pubmed
273 , 7148 - 7154 ) , we showed that overlapping peptides , N20 K (
Asn(564)-
Lys(583 ) ) and E20 K (
Glu(574)-
Lys(593 ) ) , from the catalytic domain of
phospholipase C ( PLC ) beta2 block
Gbetagamma - dependent activation of
PLC beta2 . -
pubmed
The ability of the
PLC beta2 - derived peptide to cross - link to each betagamma mutant was then analyzed to identify the reactive
sulfhydryl moiety on the beta subunit required for the cross - linking reaction . -
pubmed
This indicates that the peptide is specifically binding to a region near
cysteine 25 of
beta ( 1 ) which is located in the
amino - terminal coiled -
coil region of
beta ( 1 ) and identifies a PLC - binding site distinct from the alpha subunit interaction site . -
pubmed
The sequence of the
lens protein ' s cDNA ( about 1.7 kb total ) described an open reading frame of 582 residues which coded for a protein of 194
amino acids . -
pubmed
Modulation of radiation - induced
protein kinase C activity by
phenolics . -
pubmed
Curcumin ,
ellagic acid and
quercetin were effective in inhibiting radiation - induced
PKC activity . -
pubmed
Curcumin and
ellagic acid were found to be more inhibitory towards radiation - induced
PKC activity , while
quercetin was the least effective . -
pubmed
Curcumin was found to inhibit the activated cytosolic and particulate
PKC at very low concentrations . -
pubmed
Suppression of
PKC activity by
phenolics may be one of the means of preventing the development of radioresistance following radiotherapy . -
pubmed
Eleven different
PrP genotypes encoding at least five unique predicted mature
PrP amino acid sequences were found . -
pubmed
History , physical examination , karyotype ,
FSH ,
LH ,
testosterone , testis histology and results of ICSI using ejaculated or testis sperm were retrospectively accumulated in two academic clinical practices . -
pubmed
FSH ,
LH ,
testosterone and testis histology could not differentiate those with
oligospermia or
azoospermia , nor could they predict whether sperm could be found in harvested testis tissue . -
pubmed
CGs had plasma
catecholamine levels sampled at
rest and in response to a stressor ( speech task ) before and 1 month after a 2 - week in - home respite intervention . -
pubmed
Multidrug - resistant ( MDR ) counterparts of these cell lines express differentially the MDR - conferring
ATP - binding cassette transporter genes
MDR1 ,
MRP1 and
BCRP , respectively . -
pubmed
The N- and C - terminal
amino acids of
human hemoglobin H. -
pubmed
Linear
alkylbenzene sulfonate and
boron monitoring in four catchments in the UK contribution to GREAT -
ER # 11 . -
pubmed
3 - OH flavone inhibition of
epidermal growth factor - induced proliferaton through blocking
prostaglandin E2 production . -
pubmed
Epidermal growth factor (
EGF ) has been shown to induce proliferation in cells , however , the role of
prostaglandin E ( 2 ) (
PGE ( 2 ) ) plays in
EGF - induced proliferation in still unclear . -
pubmed
PD98059 , a specific
ERK1 / 2 inhibitor , inhibited
EGF - induced proliferation with concomitant decreases in
ERK1 / 2 phosphorylation and
COX-2 /
PGE ( 2 ) induction . -
pubmed
Non -
steroid anti - inflammatory drugs ( NSAIDs ) such as
aspirin and
diclofenac , a
COX activity inhibitor , inhibited
EGF - induced proliferation by blocking
PGE ( 2 ) production . -
pubmed
The addition of
PGE ( 2 ) reversed the inhibitory effects of
PD98059 ,
aspirin , and
diclofenac on
EGF - induced proliferation . -
pubmed
This suggests that
COX-2 /
PGE ( 2 ) activation involves in
EGF - induced proliferation and locates at the downstream of
ERK1 / 2 activation . -
pubmed
Furthermore , the natural product ,
3 - OH flavone , showed the most - potent inhibitory activity on
EGF - induced proliferation among 9 structurally - related compounds , and suppression of
EGF receptor phosphorylation ,
ERK1 / 2 phosphorylation , and
COX-2 /
PGE ( 2 ) production by
3 - OH flavone was identified . -
pubmed
PGE ( 2 ) addition attenuates the inhibitory activity of
3 - OH flavone on
EGF - induced proliferation by
MTT assay and colony formation by soft agar assay . -
pubmed
Results of our present study provide evidence to demonstrate that
PGE ( 2 ) is an important downstream molecule in
EGF - induced proliferation , and
3 - OH flavone , which inhibits
PGE ( 2 ) production by blocking
MAPK cascade , might reserve potential for development as an anti -
cancer drug . -
pubmed
Student 's t test , Tukey test and repeated measures variance analysis were used to evaluate differences in the study group and between the women with
polycystic ovary syndrome (
PCOS ) and
idiopathic hirsutism after
gonadotropin releasing hormone (
GnRH)-agonist administration . -
pubmed
The redox reaction of
cytochrome c immobilized on the bare surfaces of microelectrodes and macroscopic electrodes ( macroelectrodes ) composed of different planes of highly oriented pyrolytic
graphite has been investigated using cyclic voltammetry . -
pubmed
Markers of
inflammation ( macrophage ,
lactate dehydrogenase ,
nitric oxide ) were apparently more in the
BAL fluid of those with
lung involvement than in those without , though the
arsenic content did not differ significantly . -
pubmed
In
SOCS-3 knockdown studies , antisense
oligonucleotides inhibited the expression of
SOCS-3 and increased the
Ang II - induced
STAT activation and
c - Fos /
c - Jun expression , then resulting in a more severe
renal damage . -
pubmed
The aqueous extracts of them contained the common
seleno compound that was identified as
gamma - glutamylmethylselenocysteine by an electrospray ionization - tandem mass spectrometry ( ESI -
MS /
MS ) . -
pubmed
The role of " premature "
coronary death and the level of some risk factors , particularly
obesity and blood pressure in African descendants and high
triglycerides , low
high - density lipoproteins , and
diabetes in some Hispanics , is higher than in whites . -
pubmed
In addition , we examined the ability of
trimipramine ; a tricyclic antidepressant that is devoid of
monoamine reuptake inhibitory properties on
cytokine production . -
pubmed
All of the antidepressants suppressed
IFN - gamma production in the 10 - 50 microM concentration range , irrespective of their preference for
serotonin or
noradrenaline transporters . -
pubmed
The fact that
trimipramine also suppressed
IFN - gamma production and T - cell proliferation indicates that these immunomodulatory actions of antidepressants are most likely unrelated to inhibition of
monoamine reuptake . -
pubmed
Interestingly , exposure to a lower concentration ( 1 microM ) of the antidepressants tended to increase T - cell - derived
IL-10 production , with significant effects elicited by the
noradrenaline reuptake inhibitors
reboxetine and
desipramine . -
pubmed
After an 18 - hour preincubation in
nitrogen - free medium the accumulation ratio of
AIB exceeded 4.5 at an external
AIB concentration of 10 micromolar . -
pubmed
METHODS : The
quinolone resistance determining regions ( QRDRs ) of
gyrA ,
gyrB ,
parC and
parE of these
strains were analysed by PCR - based sequencing . -
pubmed
RESULTS : All 18
strains had at least one
amino acid substitution in the QRDRs of
GyrA and
ParC , which included
Ser-81 - ->
Tyr /
Phe and
Glu-85 - ->
Lys in
GyrA and
Ser-79 - ->
Phe /
Ile /
Tyr ,
Asp-83 - ->
Tyr ,
Asn-91 - ->
Asp ,
Ser-107 - ->
Phe ,
Lys-137 - ->
Asn and
Ala-142 - ->
Ser in
ParC. -
pubmed
Most isolates had
Asp-435 - ->
Asn /
Ile-460 - ->
Val /
Ala-596 - ->
Thr substitutions in
ParE , while no
amino acid substitution in
GyrB was noted in all isolates . -
pubmed
Ten isolates for which
levofloxacin MICs were 16 or 32 mg / L had multiple mutations in both
GyrA and
ParC. -
pubmed
We now report the discovery of an alternative and quantitatively dominant mechanism for
cyanate formation and protein carbamylation at sites of
inflammation and
atherosclerotic plaque :
myeloperoxidase - catalyzed oxidation of
thiocyanate , an anion abundant in blood whose levels are elevated in smokers . -
pubmed
We also show that
myeloperoxidase - catalyzed lipoprotein carbamylation facilitates multiple pro - atherosclerotic activities , including conversion of low - density lipoprotein into a ligand for
macrophage scavenger receptor A1 recognition ,
cholesterol accumulation and foam - cell formation . -
pubmed
Does the activity of
CuZnSOD change due to
fluoxetine treatment ? -
pubmed
The activity of
CuZnSOD rises after
fluoxetine treatment . -
pubmed
New biological roles could be attributed to various
TCSs , including roles in
cytochrome c biogenesis (
HssRS ) , transport of
carbohydrates , peptides and/or
amino acids (
YkoGH ,
LytSR ) , and resistance to toxic ions ( LiaSR ) , antimicrobial peptides (
BceRS ) and
beta - lactam antibiotics ( BacRS , YcbLM ) . -
pubmed
At higher pH
chlorin p6 was found to be photodynamically more effective in aqueous environment whereas at lower pH
chlorin p6 was photodynamically more effective in hydrophobic environment . -
pubmed
One -
pot [ 1 + 1 + 1 ] synthesis of
dithieno [ 2,3 - b : 3 ' , 2 '- d ] thiophene (
DTT ) and their functionalized derivatives for organic thin - film transistors . -
pubmed
A facile one -
pot [ 1 + 1 + 1 ] synthesis of
dithieno [ 2,3 - b : 3 ' , 2 '- d ] thiophene (; ) has been achieved , enabling the efficient realization of a new
DTT - based semiconductor series for organic thin - film transistors ( OTFTs ) . -
pubmed
When " ARDS - controlled window " appeared , the ventilation mobility was switched to oronasal
face continuous positive airway pressure ( CPAP ) , followed by CPAP until weaning ; the control group was weaned with SIMV+
PSV+PEEP . -
pubmed
This study was carried out to develop and optimize oral sustained - release formulations for
Ambroxol hydrochloride matrix pellets using a combination of wax and water - insoluble polymer ,
glyceryl behenate ( Compritol 888
ATO ) and Ethylcellulose ( EC(7
FP ) ) . -
pubmed
It involved three factors : the content of Compritol 888
ATO ( X(1 ) ) , EC(7
FP ) ( X(2 ) ) , and the matrix formation methods ( X(3 ) ) , as independent variables . -
pubmed
Medroxyprogesterone acetate also caused glomerular , vascular , tubular , and interstitial lesions that were accompanied by increased blood pressure and enhanced
NADPH oxidase (
p67phox ) and
sodium channel (
alpha - ENaC ) expression . -
pubmed
Drospirenone and
spironolactone also increased renal
angiotensin II type 2 receptor expression and relieved
aldosterone - induced suppression of serum
angiotensin II levels . -
pubmed
Evaluation of selective
gamma - secretase inhibitor
PF-03084014 for its antitumor efficacy and gastrointestinal safety to guide optimal clinical trial design . -
pubmed
We describe the potent antitumor effects of
PF-03084014 , a small molecule that is a reversible , noncompetitive , and selective
gamma - secretase inhibitor . -
pubmed
PF-03084014 treatment also resulted in robust
NICD reduction in
HBP -
ALL xenograft models . -
pubmed
Collectively , the results show that inhibition of Notch signaling by
PF-03084014 while minimizing
gastrointestinal toxicity presents a promising approach for development of therapies for
Notch receptor - dependent
cancers . -
pubmed
Using immunohistochemical techniques , we found that
GABA is colocalized with the vesicle - associated protein
synapsin in nearby nerves and hence is presumably released there . -
pubmed
Cycling
hypoxia - induced
HIF-1alpha activation reflected ROS mediated
HIF-1alpha synthesis and stabilization , whereas non - interrupted
hypoxia - induced
HIF-1alpha activation was due to decreased
HIF-1alpha degradation resulting from decreased
prolyl hydroxylation . -
pubmed
The instrument employs a microchip - based nano - LC column packed with porous graphitized
carbon (
PGC ) to provide excellent isomer separation for SHMOs with highly reproducible retention time . -
pubmed
In previous studies , low blood levels of
n-3 fatty acids (
FA ) have been associated with increased risk of
cardiac death , and the omega-3 index ( red blood cell ( RBC )
eicosapentaenoic acid (
EPA ) and
docosahexaenoic acid (
DHA ) expressed as weight percentage of total FA ) has recently been proposed as a new risk factor for
death from
coronary artery disease , especially following
sudden cardiac arrest (
SCA ) . -
pubmed
We could not demonstrate significant changes in the
FA measurements in any of the groups , this also applied to the omega-6 /
omega-3 ratio and the
arachidonic acid ( AA)/
EPA ratio . -
pubmed
GSH is synthesized into two enzymatic steps , the first , and the rate - limiting one , is catalyzed by
glutamate - cysteine ligase (
GCL ) to form a
dipeptide which is then converted to
GSH by
GSH synthetase . -
pubmed
In this chapter , we describe assays for the measurements of
GSH levels and
GCL activity . -
pubmed
Synthesis , detailed structural characterization ( X - ray , NMR ,
MS , IR , elemental analysis ) , and studies of toxicity , antioxidant activity and bioavailability of unique potent anti - atherosclerotic
succinobucol - steroid conjugates are reported . -
pubmed
A series of
pyrimidodiazepines was identified as potent
Polo - like kinase 1 (
PLK1 ) inhibitors . -
pubmed
The lead compound 7 (
RO3280 ) has potent inhibitory activity against
PLK1 , good selectivity against other
kinases , and excellent in vitro cellular potency . -
pubmed
Cyclin - dependent kinase 7 / 9 inhibitor
SNS-032 abrogates
FIP1 - like-1 platelet - derived growth factor receptor alpha and
bcr -
abl oncogene addiction in malignant hematologic cells . -
pubmed
PURPOSE : The " gate - keeper " mutations
T674I platelet - derived growth factor receptor alpha (
PDGFRalpha ) in
hypereosinophilic syndrome (
HES ) and
T315I Bcr -
Abl in
chronic myeloid leukemia (
CML ) are resistant to
imatinib and the second - generation small - molecule
tyrosine kinase inhibitors ( TKI ) . -
pubmed
The purpose of this study was to evaluate the strategy of shutting down the transcription and expression of
FIP1 - like-1 (
FIP1L1)-
PDGFRalpha and
Bcr -
Abl with
SNS-032 , an inhibitor of
cyclin - dependent kinase 7 (
CDK7 ) and
CDK9 in phase I clinical trials . -
pubmed
EXPERIMENTAL DESIGN : The effects of
SNS-032 on
PDGFRalpha and
Bcr -
Abl signaling pathways , apoptosis , and cell cycling were analyzed in TKI - resistant cells of
HES and
CML . -
pubmed
RESULTS :
SNS-032 inhibited the phosphorylation on
Ser5 and
Ser2 of
RNA polymerase II . -
pubmed
SNS-032 decreased both the mRNA and protein levels of
FIP1L1 -
PDGFRalpha and
Bcr - Abl and inhibited the proliferation of malignant cells expressing
FIP1L1 -
PDGFRalpha or
Bcr -
Abl . -
pubmed
CONCLUSIONS : This
CDK7 / 9 inhibitor potently inhibits
FIP1L1 -
PDGFRalpha - positive HES cells and
Bcr -
Abl - positive
CML cells regardless of their sensitivity to
imatinib . -
pubmed
Erufosine suppresses
breast cancer in vitro and in vivo for its activity on
PI3 K ,
c - Raf and
Akt proteins . -
pubmed
PURPOSE : This study investigated the antineoplastic effect of the membrane active
alkylphosphocholine erufosine in
breast carcinoma models in vitro and in vivo and determined its influence on the
PI3K /
Akt and
Ras /
Raf /
MAPK signaling pathways . -
pubmed
In addition ,
erufosine caused dose - dependent decreases in the phosphorylation of
PI3 K (
p85 ) ,
Akt (
PKB ) at Thr 308 and
cRaf in both cell lines . -
pubmed
In addition , it can be derived that the mechanism of action of
erufosine involves influence on both ,
PI3K /
Akt and
Ras /
Raf /
MAPK signaling pathways . -
pubmed
Voltage - gated proton channels are expressed in microglia , and are required for
NADPH oxidase - dependent reactive
oxygen species generation . -
pubmed
In addition , the
EGCG effect was preserved in the presence of the cytoskeletal stabilizers
paclitaxel and
phalloidin ,
phosphatase inhibitors , the antioxidant
Trolox ,
superoxide dismutase or
catalase . -
pubmed
Spinal cord involvement secondary to
non - Hodgkin ' s lymphoma identified by
18F - FDG PET /
CT . -
pubmed
Here , we used a
tetracycline regulated d2EGFP reporter construct containing specific 3'UTR sequences from
DDB2 to identify novel cis - acting elements that regulate mRNA stability . -
pubmed
Conversely , these same 3'UTRs led to more rapid induction of the reporter mRNA , export of the
message to the cytoplasm and the subsequent accumulation of the encoded reporter protein , indicating that this newly identified cis - acting element affects transcriptional and
post - transciptional processes . -
pubmed
After
nitrogen starvation ,
green fluorescent protein (
GFP)-
Syb1 is found in intracellular dots . -
pubmed
Electron microscopy shows that an abnormal spore wall is often formed in
syb1 -
S1 mutant spores . -
pubmed
Although most
syb1 - S1 mutant spores are germinated , they are less tolerant to
ethanol than wild - type spores . -
pubmed
The
syb1 - S1 allele carries a missense mutation , resulting in replacement of a conserved
cysteine residue adjacent to the transmembrane domain , which reduces the stability and abundance of the
Syb1 protein . -
pubmed
and their adulterants were firstly investigated and compared using
saccharide mapping , enzymatic (
endo-1,3 - beta - D - glucanase and
pectinase ) digestion followed by polysaccharide analysis using
carbohydrate gel electrophoresis analysis . -
pubmed
Cisplatin caused acute
ABR threshold shift at 30 and 40 kHz , but that acute
hearing loss led to less age - related
hearing loss at those frequencies . -
pubmed
ABR P1 amplitude input - output functions were not significantly affected by the
cisplatin exposure when controlling for threshold shift . -
pubmed
Meta - analyses demonstrated significant weighted mean difference ( WMD ) for
hsCRP ( -0.50 mg / l , 95 % CI:-0.78 ; -0.22 ) ,
IL-6 ( -0.48 ng / l , 95 % CI : -0.90 ; -0.06 ) ,
TNF - alpha ( -0.75 pg / ml , 95 % CI : -1.34 ; -0.17 ) ,
fibrinogen ( -0.47 g / l , 95 % CI : -0.76 ; -0.17 ) , total
cholesterol ( -0.11 mmol / l , 95 % CI : -0.21 ; -0.01 ) and
HDL - C ( 0.04 mmol / l , 95 % CI : 0.03 ; 0.06 ) favouring periodontal intervention . -
pubmed
Starting with the public database ChEMBL organized in the chemical space of Molecular Quantum Numbers ( MQN , a series of 42 integer value descriptors of molecular structure ) , a visual survey of nearest neighbors of the
alpha7 nAChR partial agonist
N -( 3R )- 1 - azabicyclo [ 2.2.2 ] oct-3 - yl-4 - chlorobenzamide (
PNU-282,987 ) pointed to
N -( 2 - halobenzyl )- 3 - aminoquinuclidines as possible
nAChR modulators . -
pubmed
Reducing elevated plasma LDL
cholesterol : the central role of the
LDL receptor . -
pubmed
Cyclopamine modulates
gamma - secretase - mediated cleavage of
amyloid precursor protein by altering its subcellular trafficking and lysosomal degradation . -
pubmed
Using in vitro and in vivo models , we identified
cyclopamine as a novel regulator of
gamma - secretase - mediated cleavage of
APP . -
pubmed
We demonstrate that
cyclopamine decreases
Abeta generation by altering
APP retrograde trafficking . -
pubmed
Specifically ,
cyclopamine treatment reduced
APP - C - terminal fragment (
CTF ) delivery to the trans - Golgi network where
gamma - secretase cleavage occurs . -
pubmed
Instead ,
cyclopamine redirects
APP - CTFs to the lysosome . -
pubmed
These data demonstrate that
cyclopamine treatment decreases
gamma - secretase - mediated cleavage of
APP . -
pubmed
In addition ,
cyclopamine treatment decreases the rate of
APP -
CTF degradation . -
pubmed
Together , our data demonstrate that
cyclopamine alters
APP processing and
Abeta generation by inducing changes in
APP subcellular trafficking and
APP -
CTF degradation . -
pubmed
There are 2 main cotransporters in the kidney ,
SGLT1 and
SGLT2 , which reabsorb the
glucose ( 10 % and 90 % , respectively ) and return it to the blood . -
pubmed
Selective pharmacological
SGLT2 inhibition increases renal
glucose excretion and secondarily reduces its plasma values . -
pubmed
Several clinical trials have shown that
dapagliflozin ( 10mg / day ) , the first
SGLT2 inhibitor commercialized in Spain , produces a statistically significant reduction in HbA1c of 0.82 - 0.97 % , both in monotherapy and in combination with
metformin ,
glimepiride ,
pioglitazone , or
insulin . -
pubmed
Phospholipase C (
PLC ) comprises a superfamily of enzymes that play a key role in a wide array of intracellular signalling pathways , including
protein kinase C and intracellular
calcium . -
pubmed
Exploring the
copper ( II )- aminotriazole complex - binding sites of
human serum albumin . -
pubmed
Further fluorescent tests revealed that the quenching mechanism of
HSA by
Cu ( II )- ATA was overall static . -
pubmed
Meanwhile , the obtained binding constant and thermodynamic parameters on complex -
HSA interaction showed that the types of interaction force of
Cu ( II )- ATA and
HSA were
hydrogen bonding , van der Waals and electrostatic . -
pubmed
The analysis of three - dimensional fluorescence , circular dichroism and Fourier transform infrared spectroscopy showed that
Cu ( II )- ATA induced the changes in the secondary structure of
HSA . -
pubmed
Furthermore ,
amino group and attractive electrostatic interaction of
Cu ( II )- ATA greatly contributed to the
hydrogen bonding , van der Waals and electrostatic interaction between
Cu ( II )- ATA and
HSA , as confirmed by experimental data . -
pubmed
In our work , a combination of surface photovoltage spectroscopy (
SPS ) , transient photovoltage ( TPV ) technique , photoelectrochemical impedance spectroscopy ( PEIS ) and transient photocurrent measurements was used to study the real role of
Ni ( OH ) 2 as a cocatalyst for the enhanced PEC performance of
Ni ( OH ) 2 - modified
Ti - doped alpha - Fe2O3 . -
pubmed
Discovery of
( ( 4 -( 5 -( Cyclopropylcarbamoyl )- 2 - methylphenylamino )- 5 - methylpyrrolo [ 1,2 - f ][ 1,2,4 ] triazine-6 - carbonyl )( propyl ) carbamoyloxy ) methyl-2 -( 4 -( phosphonooxy ) phenyl ) acetate (
BMS-751324 ) , a Clinical Prodrug of
p38alpha MAP Kinase Inhibitor . -
pubmed
In search for prodrugs to address the issue of pH - dependent solubility and exposure associated with 1 (
BMS-582949 ) , a previously disclosed phase II clinical
p38alpha MAP kinase inhibitor , a structurally novel clinical prodrug , 2 (
BMS-751324 ) , featuring a
carbamoylmethylene linked promoiety containing
hydroxyphenyl acetic acid (
HPA ) derived
ester and
phosphate functionalities , was identified . -
pubmed
In this study , we show that
curcumin at low concentrations ( 1.25 - 3.12 muM ) has a strong anti - proliferative effect on
TNF - alpha - induced
psoriasis - like
inflammation when applied in combination with light - emitting - diode devices . -
pubmed
Intraoperative peak
glucose value and
insulin requirement have also been reported to be lower with del Nido cardioplegia , which can have prognostic significance . -
pubmed
The 3 bp in - frame deletion removes 1 of the 3 adjacent
isoleucine residues in transmembrane segment DIVS6 of
Nav1.6 ( p. Ile1750del ) . -
pubmed
The third - generation
aromatase inhibitor
letrozole inhibits
estrogen production , and is more efficacious than the
estrogen receptor inhibitor
tamoxifen . -
pubmed
Recent studies have demonstrated that endogenous
TRPV1 agonists are generated by oxidation of
omega-6 polyunsaturated fatty acids , including both
linoleic acid and
arachidonic acid . -
pubmed
Together , these studies point to a novel
pain mechanism involving the enzymatic generation of endogenous
OLAM and
OAAM agonists of
TRPV1 . -
pubmed
Furthermore , commensal bacteria contribute to intestine epithelial homeostasis through
NOX1- and
dual oxidase 2 - derived reactive
oxygen species ( ROS ) . -
pubmed
The overproduction of
superoxide anion together with inactivation of
superoxide dismutase are involved in the pathogenesis of
Barrett ' s esophagus and its transformation to
adenocarcinoma . -
pubmed
In
colorectal cancer , redox signaling exhibits two Janus
faces : On the one hand ,
NOX1 up - regulation and derived
hydrogen peroxide enhance Wnt /
beta - catenin and Notch proliferating pathways ; on the other hand , ROS may disrupt
tumor progression through different pro - apoptotic mechanisms . -
pubmed
Involvement of
Glucagon - Like Peptide-1 in the Regulation of Selective Excretion of
Sodium or
Chloride Ions by the Kidneys . -
pubmed
The increase in
GLP-1 level was similar to that after oral
glucose administration . -
pubmed
After intraperitoneal administration of 2.5 %
NaCl ,
GLP-1 mimetic
exenatide accelerated natriuresis and urinary
chloride excretion . -
pubmed
These findings suggest that
GLP-1 participates in selective regulation of the balance of
sodium and
chloride ions . -
pubmed
CD169 is a
Siglec that may function as an adhesion molecule and a facilitator of the recognition and internalization of
sialic acid decorated apoptotic bodies and exosomes derived from
tumors . -
pubmed
Abiraterone acetate is the oral prodrug of
abiraterone , a specific
CYP17 inhibitor that blocks
androgen biosynthesis within the adrenal glands , testes and
tumor microenvironment . -
pubmed
Keywords used for the search were
erectile dysfunction ,
phosphodiesterase type 5 inhibitors ,
sildenafil ,
vardenafil ,
tadalafil ,
avanafil , safety , side effects , and adverse events , among others . -
pubmed
Stimulation of renal collecting duct principal cells with
antidiuretic hormone (
arginine - vasopressin ,
AVP ) results in inhibition of the
small GTPase RhoA and the enrichment of the
water channel aquaporin-2 (
AQP2 ) in the plasma membrane . -
pubmed
Rho guanine nucleotide exchange factors (
GEFs ) interact with
RhoA , catalyze the exchange of
GDP for
GTP and thereby activate the
GTPase . -
pubmed
When comparing
Orx vs.
Orx+G groups the following significant ( P<0.05 ) changes were observed : a further increase in number of replicating cells in zonas glomerulosa and reticularis , vasculature network presence , cortical and zona reticularis volumes , ACTH and
corticosterone concentrations , and lower
DHEA levels . -
pubmed
Comparing
Orx vs.
Orx+Soy resulted in elevated ( P<0.05 )
ACTH and
corticosterone levels . -
pubmed
However , the
CP with 2 %
KOH combined with 1 %
Ca ( OH ) 2 displayed similar effects to those of the MP with 3 %
KOH , obtaining the cumulative
methane yield of 239.8 mL gVS-1 and an improved biodegradability from 56.37 % of raw WS to 66.10 % . -
pubmed
Moreover , the results of thyroid function tests ( free
triiodothyronine , free
thyroxine , and
thyroid - stimulating hormone ) remained within normal ranges both before and after ablation . -
pubmed
In addition , blood viscosity and serum lipid assays suggested that XFZY capsules could decrease serum
triglycerides , total
cholesterol ,
low - density lipoprotein cholesterol and whole blood viscosity at a low shear rate . -
pubmed
An
antilisteriolysin O ( LLO ) monoclonal antibody (
HID5E12D7 ;
IgG2b ) covalently bound to
polystyrene amidine - modified latex beads was used in a slide agglutination assay . -
pubmed
The latex reagent detected 0.1 ng / ml of
LLO in
phosphate - buffered saline plus
bovine serum albumin . -
pubmed
We observed no effect on intramuscular proteins involved in lipolysis (
ATGL ,
G0S2 ,
CGI-58 ,
HSL ) or
fatty acid transport and capacity ( CD-36 and
HAD , respectively ) . -
pubmed
In this study , we developed a nanodisc ( ND)-functionalized bioelectronic nose (
NBN ) based on a
nickel (
Ni)-decorated carboxylated
polypyrrole nanoparticle ( cPPyNP)-FET that offers the detection of liquid and gaseous
cadaverine ( CV ) . -
pubmed
The
NBN was fabricated by the oriented assembly of
TAAR13c - embedded NDs ( T13NDs ) onto the transistor with
Ni / cPPyNPs . -
pubmed
During the last decade ,
ulipristal acetate (
UPA ) , a selective
progesterone receptor modulator , has emerged as the most effective EC pill , and it is now recommended as first line hormonal treatment for
EC in several countries . -
pubmed
In the present review , we summarize and discuss the existing evidence obtained on the effect of
UPA on sperm function and
post - ovulatory events as potential additional mechanisms to prevent pregnancy . -
pubmed
Thus , besides the described effects on ovarian function ,
UPA contraceptive effectiveness might also be attributed to
post - ovulatory effects , depending on the moment of the female cycle in which the drug is administered . -
pubmed
We observed profound
prostate - specific antigen (
PSA ) and/or objective responses to immune checkpoint blockade following prior treatment with bipolar
androgen therapy ( BAT ) and
enzalutamide . -
pubmed
Synthesis , Antibacterial and
Lipoxygenase Inhibition Studies of
N -( Alkyl / aralkyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfonamides . -
pubmed
Moreover ,
N -( 3 - phenylpropyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfonamide ( 5c ) and
N -( 4 - chlorobenzyl )- N -( 2,3 - dihydro-1,4 - benzodioxin-6 - yl )- 4 - methylbenzenesulfon - amide ( 5e ) displayed decent inhibition against
lipoxygenase enzyme relative to standard
Baicalein . -
pubmed
Hydrothermal carbonization can play an innovative role in sewage sludge (
SS ) treatment and valorization , as well as in
phosphorus recovery . -
pubmed
In this study , leaching tests using
nitric acid were performed on hydrochar from
SS and the influence of pH ( 1 - 3.5 ) , leaching time ( 30 - 240 min ) , and solid / liquid ( S / L ) ratio ( 5 - 20 wt% ) was analyzed and optimized according to the Design of Experiments method , under the Response Surface Methodology approach . -
pubmed
( 2 - Aminobenzothiazole )- Methyl-1,1 - Bisphosphonic Acids : Targeting
Matrix Metalloproteinase 13 Inhibition to the Bone . -
pubmed
Combining both approaches , we conducted the synthesis and preliminary biological evaluation of a series of
( 2 - aminobenzothiazole )- methyl-1,1 - bisphosphonic acids active as selective inhibitors of
MMP-13 via in vitro and in silico studies , which could prove useful for the treatment of
bone metastases thanks to the bone - targeting capabilities granted by the
bisphosphonic acid group . -
pubmed
Effect of
sodium - glucose cotransporter-2 inhibitors on renal handling of
electrolytes . -
pubmed
In this review , we aim to describe
electrolytes and acid - base abnormalities due to
SGLT2i as well as to elucidate the underlying mechanism . -
pubmed
Changes in bone mineral composition and plasma
Ca ,
Mg and F were determined as well as alterations in
bone pyrophosphatase and the amounts of bone
cyclic-3 ' , 5 '- adenosine monophosphate (
cAMP ) . -
pubmed
The levels of soluble
bone pyrophosphatase were not greatly influenced by changes in dietary
fluoride . -
pubmed
Administration of
EHDP at 20 mg P / kg , however , typically decreased the levels of
bone pyrophosphatase . -
pubmed
At 1 mM
acetate 10 % of the cell
carbon came from
acetate , the
rest from
CO2 . -
pubmed
Degradation studies showed that
C1 of
alanine ,
C1 and
C4 of
aspartate , and
C1 and
C5 of
glutamate were exclusively derived from
CO2 , whereas
C2 and
C3 of
alanine and
aspartate , and C3 and
C4 of
glutamate were partially derived from
acetate . -
pubmed
The [
Ca2 +] i response of the three clones to
BK decreased with the receptor number / cell . -
pubmed
BK at 10nM and
thrombin at 2units / ml both effectively increased cytosolic [
Ca2+]i . -
pubmed
BK at 10nM stimulated
PGE2 production three fold over basal , while
thrombin only marginally elevated
PGE2 levels . -
pubmed
Alone ,
BK stimulated a small increase in
3H - thymidine incorporation into DNA . -
pubmed
This peptide processing can be specifically blocked by the
angiotensin-1 converting enzyme (
ACE ) inhibitor
captopril , and can occur by exposing
p18 to purified
ACE . -
pubmed
Blood pressure and
sodium concentrations in CSF and serum were significantly higher in the high salt period than the low salt period (
CSF Na+ concentration : 147.7 + /- 0.4 mmol / L vs 145.3 + /- 0.5 mmol / L ; P less than 0.001 ) . -
pubmed
Levels of
CSF pressure and
potassium or
calcium concentrations were not different between the two periods . -
pubmed
The levels and the changes in
sodium and NA in
CSF were not significantly different between the salt - sensitive ( n = 8) and the non - salt - sensitive ( n = 7 ) subjects , but the changes in plasma NA and PRA were smaller in the salt - sensitive subjects . -
pubmed
Decreased
5 - HT - mediated
prolactin release in
major depression . -
pubmed
Glycolytic oscillations were triggered after a
pulse of
glucose by inhibition of mitochondrial respiration with
KCN ,
myxothiazol and
antimycin A or in mutants in the
bc1 complex (
ubiquinol : cytochrome c reductase ) that were largely deficient in respiratory capacity . -
pubmed
Inhibition of the
adenine nucleotide translocator by preincubation with
bongkrekic acid also triggered a train of damped sinusoidal oscillations after
glucose addition . -
pubmed
Low rates of cytosolic
NADH reoxidation induced by
pyrazole , an inhibitor of
alcohol dehydrogenase , were also associated with the disappearance of glycolytic oscillations . -
pubmed
The mitochondrial
NADH dehydrogenase and the glycolytic steps associated with
NADH reoxidation downstream from
pyruvate appear to provide another control level of glycolysis dynamics in vivo . -
pubmed
Extensive phosphorylation of
pp60c - src occurs at
tyrosine 527 in fibroblasts and inhibits
pp60c - src kinase activity . -
pubmed
These data suggest that
pp60c - src in fibroblasts is normally inhibited by trans - acting factors , that either directly catalyze
phosphate transfer to
tyrosine 527 or augment autophosphorylation at this site . -
pubmed
When log - phase cells growing in
glucose were heat shocked ,
hsp26 concentrated in nuclei and continued to concentrate in nuclei when these cells were returned to normal temperatures for recovery . -
pubmed
Similarly , in cells genetically engineered to synthesize
hsp26 in the presence of
galactose ,
hsp26 did not concentrate in nuclei , with or without a heat
shock . -
pubmed
In wild - type cells grown in
galactose or
acetate and in mitochondrial mutants grown in
glucose or
galactose ,
hsp26 also failed to concentrate in nuclei with a heat
shock . -
pubmed
Quantitative measurement of [
3H]
acetylcholine synthesis and storage from
[ 3H ] choline were negative at the early neurula stage and in 5 to 15 - day - old NF cultures , and remained low in 5 to 15 - day - old
NP cultures . -
pubmed
Acetylcholine production in cocultures increased linearly with time and was always much higher than in
NP cultures . -
pubmed
Mortality was significantly higher in group
IV which also showed doubling of myocardial
malondialdehyde content relative to the non -
adriamycin - treated
vitamin E deficient group ( III ) . -
pubmed
Total
sialic acid content of the sarcolemma in groups
II , III and
IV was 55 , 90 and 24 % of the control values in group I. -
pubmed
The two major transcripts contain
NGF ( 12.5 kilodaltons [ kDa ] ) at the C - terminus and differ by alternative splicing of an N - terminal exon , so that the large precursor ( 34 kDa ) had 67
amino acids upstream of an internal signal peptide and the smaller precursor ( 27 kDa ) had this signal peptide at its N - terminus . -
pubmed
In these cells , a membrane - permeable
cyclic AMP analog ,
8 - bromo - cyclic AMP , stimulated
NGF secretion . -
pubmed
The last two steps in the purification of this antibiotic included reverse - phase chromatography on
C18 - bonded
silica gel and countercurrent chromatography on an Ito Coil Planet Centrifuge to give material of 90 % purity . -
pubmed
Molecular dynamics simulation of photodissociation of
carbon monoxide from
hemoglobin . -
pubmed
A molecular dynamics simulation of the photodissociation of
carbon monoxide from the alpha subunit of
hemoglobin is described . -
pubmed
The effect of the
protein on the rate and extent of the displacement of the
iron from the
porphyrin plane was assessed by comparing the results with those obtained for an isolated complex of
heme with
imidazole and
carbon monoxide . -
pubmed
The half - time for the displacement of the
iron from the
porphyrin plane was found to be 50 - 150 fs for both the
protein and the isolated complex . -
pubmed
These results support the interpretation of optical absorption studies using 250 - fs laser
pulses that the
iron is displaced from the
porphyrin plane within 350 fs in both
hemoglobin and a free
heme complex in solution . -
pubmed
The new peptide had an apparent molecular weightof 4000 daltons and was more acidic than
ACTH as analyzed by
sodium dodecyl sulfate /
urea and acidic ( pH 4.5 ) polyacrylamide gel electrophoreses , respectively . -
pubmed
High performance liquid chromatographic analysis of
[ 35S ] methionine - labeled tryptic fragments of the peptide revealed that this peptide contained
gamma MSH - ( 1 - 7 ) within its sequence . -
pubmed
In the group given 0.2 mg / kg for 90 days and that given 1.0 mg / kg for 9 or 30 days , free
ACh content increased slightly but significantly , and
AChE activity decreased to 58 per cent . -
pubmed
The
amino acid composition of this protein was different from those of
AA protein . -
pubmed
Addition of
adenosine 3 ' , 5 '- cyclic monophosphate (
cAMP)-elevating agents [
forskolin and
3 - isobutyl-1 - methylxanthine ( I / F ) ] led to large increases in uptake of
36Cl ,
125I , and
82Br into oocytes expressing wild - type
CFTR or delta F508
CFTR but not sham - injected oocytes . -
pubmed
I / F also stimulated
halide efflux from
CFTR and delta F508 oocytes in the sequence Cl & gt ; Br & gt ; I. -
pubmed
cAMP - induced increases in
36Cl efflux from delta F508 oocytes were approximately 20 % of those in
CFTR oocytes . -
pubmed
The
phorbol ester ,
phorbol 12 - myristate 13 - acetate , also stimulated
36Cl efflux from
CFTR oocytes . -
pubmed
ATP uptakes into
CFTR and sham oocytes were similar , and both were reduced by I / F. However ,
ATP uptake into I / F - treated
CFTR oocytes was slightly greater ( approximately 40 % ) than into I / F - treated sham oocytes . -
pubmed
Urea uptake into
CFTR and sham oocytes was similar and in both cases was increased by I / F. However , the I / F - induced increase in
urea uptake into
CFTR oocytes was significantly greater than for sham oocytes . -
pubmed
I / F stimulated
formate uptake into
CFTR oocytes but not into sham oocytes . -
pubmed
Fluxes of
22Na ,
86Rb ,
35SO4 ,
32PO4 , and
mannitol were unaltered by expression and activation of
CFTR . -
pubmed
In both cell models , in the presence of
cGMP and
ATP ,
cGKII was found to mimic the effect of the catalytic subunit of
cAMP - dependent protein kinase (
cAK ) on opening
CFTR -
Cl - channels , albeit with different kinetics ( 2 - 3 - min lag time , reduced rate of activation ) . -
pubmed
By contrast ,
cGKI or a monomeric
cGKI catalytic fragment was incapable of opening
CFTR -
Cl- channels and also failed to potentiate
cGKII activation of the channels . -
pubmed
The slow activation by
cGKII could not be ascribed to counteracting
protein phosphatases , since neither
calyculin A , a potent inhibitor of
phosphatase 1 and 2A , nor
ATP gamma S (
adenosine 5 '- O -( thiotriphosphate ) ) , producing stable thiophosphorylation , was able to enhance the activation kinetics . -
pubmed
Channels preactivated by
cGKII closed instantaneously upon removal of
ATP and
kinase but reopened in the presence of
ATP alone . -
pubmed
Paradoxically , immunoprecipitated
CFTR or
CF-2 , a cloned R domain fragment of
CFTR (
amino acids 645 - 835 ) could be phosphorylated to a similar extent with only minor kinetic differences by both isotypes of
cGK . -
pubmed
These results indicate that
cGKII , in contrast to
cGKI alpha , is a potential activator of
chloride transport in
CFTR - expressing cell types . -
pubmed
In contrast , activity of the adrenal
catecholamine - synthesizing enzyme
tyrosine hydroxylase , but not
phenylethanolamine - N - methyl transferase , was significantly elevated in CHR compared to CTL . -
pubmed
Fluticasone propionate is a new , topically active
glucocorticoid which shows high specificity for the
glucocorticoid receptor . -
pubmed
In vitro addition of
AS101 at 0.1 micrograms / ml to the in vivo UVB - treated lymphocytes raised
IL-2 production to approximately double the initial level , and improved the helper activity of
CD4 cells . -
pubmed
The
insulin gene contains multiple transcriptional elements that respond to
glucose . -
pubmed
The beta cells in the pancreatic islets of Langerhans increase
insulin gene transcription in response to increased
glucose concentration . -
pubmed
We have mapped sequences within the
rat insulin I gene 5 '- flanking DNA ( rInsI promoter ) that direct this transcriptional response to
glucose . -
pubmed
A 50 - bp sequence isolated from the
rInsI promoter , the Far - FLAT minienhancer , can confer
glucose responsiveness to nonresponsive promoters . -
pubmed
Nuclear extracts from islets grown in various
glucose concentrations demonstrate a
glucose - stimulated increase in a protein complex that binds the Far element and contains the transcription factors
Pan-1 and
Pan-2 . -
pubmed
Overexpression of intact or partially deleted
Pan-1 ablates the Far - directed transcriptional response to
glucose . -
pubmed
We conclude that the full
glucose response of the
insulin promoter involves the interaction of multiple sequence elements . -
pubmed
A single transgene , when placed in a
catalase null background , was sufficient to restore resistance to
H2O2 as well as reduce susceptibility to early
death . -
pubmed
The
Drosophila catalase gene is composed of 3 exons , encoding 19 , 307 , and 180
amino acids , which are separated by 3520- and 96 - bp introns . -
pubmed
Sequence analysis of the promoter domain is presented , revealing multiple sequence similarities between
catalase and
Cu ,
Zn superoxide dismutase promoter domains . -
pubmed
Developmental RNA get analysis shows that peaks of
catalase mRNA accumulation correspond roughly with major peaks of
ecdysone titer during third instar and pupal stages . -
pubmed
Candidate
ecdysone response element sequences are noted downstream of the
catalase polyadenylation site . -
pubmed
Addition of free radical scavengers such as ,
superoxide dismutase ( 100 units / ml ) ,
catalase ( 150 units / ml ) ,
mannitol ( 1 mM ) and
beta - carotene ( 0.5 mM ) to the reaction system showed an insignificant effect on lipid peroxidation . -
pubmed
In this system a concomitant increase in the activity of microsomal
glutathione - S - transferase was observed which may serve as an alternative pathway to detoxify lipid
peroxides . -
pubmed
Addition of
glutathione alone to the reaction system prevented both against the loss in
vitamin E content and increase in the activity of
glutathione - S - transferase . -
pubmed
Supplementation of both
vitamin E and
glutathione was found to be effective in lowering
glutathione - S - transferase activity to that of normal basal level . -
pubmed
Our data further suggest that the lung tissue may have an inbuilt mechanism whereby
glutathione - S - transferase may be triggered to cope with the excessive production of lipid
peroxides . -
pubmed
The only clearly identifiable cells that were
[ 3H ] thymidine - positive were astrocytes , based on
GFAP staining . -
pubmed
The normal BBB is relatively permeable to water , but considerably less so to ions , including the principal
electrolytes Brain fluid regulation takes place within the context of systemic fluid volume control , which depends on the mutual interaction of osmo- , volume- , and pressure - receptors in the hypothalamus , heart and kidney , hormones such as
vasopressin ,
renin -
angiotensin ,
aldosterone ,
atriopeptins , and digitalis - like immunoreactive substance , and their respective sites of action . -
pubmed
Left ventricular wall thickness was measured by echocardiography , and relative amounts of
myosin heavy chain isoforms were assessed by
pyrophosphate gels . -
pubmed
However , the decrease in
systolic blood pressure caused by
nifedipine continued for no more than 8 hours , whereas the blood pressure - lowering effect of
amlodipine continued for more than 16 hours
post dose . -
pubmed
Amlodipine , but not
nifedipine , prevented an increase in the relative amount of
V3 myosin heavy chain isoform and suppressed an increase in mRNA levels of
beta - myosin heavy chain ,
skeletal alpha - actin , and
type 1 collagen . -
pubmed
An assay has been developed that allows the measurement of remnant - like particle
cholesterol ( RLP - C ) and
triglyceride (
RLP - TG ) . -
pubmed
The inhibitory effects of
nucleotides are mediated via the pore - forming subunit ,
Kir6.2 , whereas the potentiatory effects are conferred by the
sulfonylurea receptor subunit ,
SUR . -
pubmed
The stimulatory action of
Mg - nucleotides complicates analysis of
nucleotide inhibition of
Kir6 . -
pubmed
We therefore used a truncated isoform of
Kir6.2 , that expresses
ATP - sensitive channels in the absence of
SUR1 , to explore the mechanism of
nucleotide inhibition . -
pubmed
We found that
Kir6.2 is highly selective for
ATP , and that both the
adenine moiety and the
beta - phosphate contribute to specificity . -
pubmed
The emission spectrum was excited in a
Be hollow cathode discharge lamp with a
He /
D2 gas mixture . -
pubmed
The osteolytic process is mainly characterized by an osteoclastic activity of
bone resorption and inflammatory activity provoked by various
cytokines and
prostaglandins . -
pubmed
Exploring structure - activity relationships around the
phosphomannose isomerase inhibitor
AF14049 via combinatorial synthesis . -
pubmed
Solid - phase synthetic methods were developed and a series of libraries and discrete analogs synthesized to explore
SAR around
AF14049 . -
pubmed
Thymidine dinucleotide mimics the effect of solar simulated irradiation on
p53 and
p53 - regulated proteins . -
pubmed
We find that , like UV irradiation ,
pTpT increases the levels of
p53 ,
p21 , and
proliferating - cell nuclear antigen . -
pubmed
The total group population with normal
calcium intake ( & gt ; 1,000 mg / day ) showed higher
BMD ( proximal femur and spine ) than the group with low
calcium intake , this variation not being observed in group BB alleles . -
pubmed
CONCLUSIONS : Our results indicate an effect of the
VDR genotype on
BMD proximal femur which is clearly influenced by
calcium intake and
calcidiol serum levels . -
pubmed
Phenylarsine oxide , which inhibits the translocation of
glucose transporters , decreased
brazilin - stimulated
glucose transport to the basal level . -
pubmed
The inhibition of
phosphatidylinositol 3 - kinase (
PI3 - kinase ) with
wortmannin also blocked
brazilin - stimulated
glucose transport . -
pubmed
Western blot analysis with an anti -
GLUT4 antibody revealed that
brazilin increased the translocation of
GLUT4 from intracellular pools to the plasma membrane . -
pubmed
Protein kinase C activity was not influenced by
brazilin treatment . -
pubmed
The inhibition of protein synthesis showed no effect on
brazilin - stimulated
glucose transport , and
GLUT4 content in the total membrane fraction was not altered as a result of treatment with
brazilin for 4 hr . -
pubmed
Metabolic labeling of
GLUT4 with
[ 35S ] methionine showed that de novo synthesis of
GLUT4 was not induced by
brazilin . -
pubmed
These data suggest that
brazilin may increase
glucose transport by recruitment of
GLUT4 from intracellular pools to the plasma membrane of adipocytes via the activation of
PI3 - kinase . -
pubmed
However , the effect of
brazilin may not be mediated by
GLUT4 synthesis and
protein kinase C activation . -
pubmed
Genetic polymorphism in either
CYP1A1 or
glutathione S - transferase is linked to an increased risk of smoking related
cancers , while
N - acetyltransferase activity is related to
cancers in which
aromatic amines are the main risk factor . -
pubmed
In contrast , a supramaximal dose of
indomethacin ( 6 mg / kg ) partially inhibited , and
dexamethasone completely prevented the carrageenan - induced increase in
NGF . -
pubmed
BACKGROUND : The
tetra - acetate ester of
2 - deoxy - D - glucose was recently found to either inhibit or augment
insulin secretion , depending on the concentration of the
ester . -
pubmed
The precise role of apoptosis in the pathogenesis of
hepatitis C - related liver injury remains unclear , but its induction may be related to downregulation of
bcl-2 expression associated with
ethanol consumption . -
pubmed
Efficient measurement of
( 3 ) J(N , Cgamma ) and
( 3 ) J ( C ' , Cgamma ) coupling constants of aromatic residues in
( 13 ) C ,
( 15 ) N - labeled
proteins . -
pubmed
Light induces phosphorylation of
photosystem II (
PSII ) proteins in
chloroplasts by activating the
protein kinase(s ) via reduction of
plastoquinone and the
cytochrome b ( 6 ) f complex . -
pubmed
( ii ) A
disulfide bond in the
LHCII kinase , rather than in its substrate , may be a target component regulated by
thioredoxin . -
pubmed
( iii ) The target
disulfide bond in inactive
LHCII kinase from dark - adapted leaves is exposed and easily reduced by external
thiol mediators , whereas in the activated
LHCII kinase the regulatory
disulfide bond is hidden . -
pubmed
( iv ) Upon high - light illumination of leaves , the target
disulfide bond becomes exposed and thus is made available for reduction by
thioredoxin , resulting in a stable inactivation of
LHCII kinase . -
pubmed
Metal - binding properties of an engineered purple
CuA center in
azurin . -
pubmed
Harder metal ions , like
Co ( II ) , bind to
apo - CuA - azurin only under basic conditions ( pH 9.1 - 9.2 ) . -
pubmed
The results obtained from these studies indicate that two factors influence metal binding in
CuA azurin : ( 1 ) the site favors metal combinations which produce an overall +3 charge , and ( 2 ) the site binds soft , thiophilic metal ions . -
pubmed
The maximal specific extracellular
glucose oxidase production ( 3.17 U mg-1 dry cell mass ) was achieved when the initial dissolved
oxygen concentration was 6.83 mg l-1 at the impeller speed of 420 rev min-1 and at the rate of aeration of 0.25 vvm . -
pubmed
Ang II was infused when subjects were in balance on a 10 - mmol
Na (+ ) intake . -
pubmed
rh - DNase ,
rh - GH ,
rh - MetGH , and
rh - IGF - I were freeze dried with either
mannitol ,
sucrose ,
trehalose , or dextran at concentrations ranging from 0 % to 100 % ( w / w ) . -
pubmed
Melting of
mannitol was observed through almost all of the
protein concentration range examined . -
pubmed
Two melting endotherms , however , were observed for
mannitol at most
protein /
mannitol ratios , indicating the presence of protein /
mannitol interactions . -
pubmed
This work suggests that direct interactions occur between
proteins and
carbohydrates in lyophilized mixtures . -
pubmed
The
protein component accounted for between 0.5 and 2.0 % of the AGPs and contained between 7 and 12 %
hydroxyproline . -
pubmed
HSP induction correlated with enhanced survival of neonatal cardiomyocytes after sublethal heat stress in
XTT testing . -
pubmed
The burst - generating mechanism in the model neuron results from the interaction of a
N - methyl - D - aspartate (
NMDA)-induced current and the
sodium pump . -
pubmed
Cytosine deaminase/
5 - fluorocytosine system had significant antitumoractivities in vivo . -
pubmed
In addition , the solution structure of peptide 1 that contains a
phenylalanine residue ( F186 in
hCD81 ) known to be critical in the binding interaction with
HCV - E2 was determined using 1D and 2D
1H NMR spectroscopy . -
pubmed
The
CD data recorded as a function of pH and
NaCl concentration are consistent with stabilization of the helical structure from electrostatic forces for both peptides . -
pubmed
BACKGROUND / PURPOSE : This randomized study examined survival ( S ) and event - free survival (
EFS ) rates using high - or standard - dose
cisplatin - based combination chemotherapy and surgical resection for this subset of
germ cell tumors . -
pubmed
Recombinant nucleoprotein productivity in nutritional feeding cultures exposed to hyperosmolarity was about 72 % higher than that obtained in batch culture at physiological osmolarity , but 31 % was a result of feeding and the
rest 41 % was a result of hyperosmolarity and increasing
Na (+ ) concentration . -
pubmed
[ Role of
sulfhydryl groups in
antitrypsin in serum ] . -
pubmed
The homo - oligomer III was isolated following
SDS treatment of
ATP synthase , the proton - turbine III+IV was obtained by blue - native electrophoresis , and complete
CFO was isolated by anion exchange chromatography of
NaSCN splitted
ATP synthase . -
pubmed
HVR-1 quasispecies modifications occur early and are correlated to initial but not sustained response in
HCV - infected patients treated with pegylated- or standard - interferon and
ribavirin . -
pubmed
L - Arginine is a common substrate for the enzymes
arginase and
nitric oxide synthase (
NOS ) . -
pubmed
Arginase is activated by
manganese (
Mn ) , and the consumption of a
Mn - deficient (
Mn- ) diet can result in low
arginase activity . -
pubmed
Tension produced by
NOS inhibition using
N ( G ) monomethyl - L - arginine (
L - NMMA ; 10(-3 ) M ) and vasorelaxation evoked by ( i )
arginase inhibition using
difluoromethylornithine (
DFMO ; 10(-7 ) M ) , ( ii )
ACh ( 10(-8)-10(-4 ) M ) in the presence of
DFMO , and ( iii )
sodium nitroprusside ( 10(-9)-10(-4 ) M ) were unaffected by diet . -
pubmed
In vitro - measured
NOS activities allowed the estimation of cardiomyocyte capacity for
NO production , a value that increased from 57 % ( sea level ) to 79 nmol NO.min(-1).g heart(-1 ) ( 4,340 m ) . -
pubmed
The contribution of
mtNOS to total cell
NO production increased from 62 % ( sea level ) to 71 % ( 4340 m ) . -
pubmed
Heart
mtNOS activity showed a linear relationship with hematocrit and a biphasic quadratic association with
estradiol and
testosterone . -
pubmed
Multivariate analysis showed that exposure to high altitude linearly associates with hematocrit and heart mtNOS activity , and that
testosterone - to -
estradiol ratio and heart weight were not linearly associated with
mtNOS activity . -
pubmed
Remifentanyl led to an improvement over the local anaesthetic technique previously used , with a clear decrease in intra- and
post - operative neurovegetative symptoms such as
dizziness ,
nausea ,
vomiting and
pain , as well as reduced
bleeding . -
pubmed
AhR - active compounds were isolated from sediments by Soxhlet extraction , and the crude extracts were fractionated using a
Florisil column into three Fractions . -
pubmed
It was determined that sediments from the Dagu River contained greater concentrations of
TCDD - EQ than did sediments from the Haihe River except at Jingangqiao ( location
R3 ) , which is associated with industrial activities in the adjacent densely populated area . -
pubmed
With confocal microscopy , we demonstrated that
calcium rise , induced by the perifusion of a solution containing a high
potassium concentration , was higher in
SolC1(- ) than in
SolD(+ ) myotubes . -
pubmed
The analysis of amplitude and kinetics of the
calcium increase in
SolC1(- ) and in
SolD(+ ) myotubes during the exposure with SR
Ca2 + channel inhibitors (
ryanodine and
2 - APB ) suggested the presence of two mechanisms of SR
calcium release : ( 1 ) a fast SR
calcium release that depended on
ryanodine receptors and ( 2 ) a slow SR
calcium release mediated by
IP3 receptors . -
pubmed
Furthermore , analysis of the kinetics of the rise in
calcium revealed that the slow
IP3 - dependent release may be increased in the
SolC1(- ) as compared to the
SolD(+ ) , suggesting an inhibitory effect of mini -
dystrophin in this signaling pathway . -
pubmed
Upon incubation with
pertussis toxin (
PTX ) , an inhibitory effect similar to that of the
IP3R inhibitor (
2 - APB ) was observed on K+-evoked
calcium release . -
pubmed
A hypothetical model is depicted in which both
Gi protein and
IP3 production could be involved in K+-evoked
calcium release as well as a possible interaction with mini -
dystrophin . -
pubmed
Our findings demonstrate the existence of a potential relationship between mini -
dystrophin and SR
calcium release as well as a regulatory role of mini -
dystrophin on intracellular signaling . -
pubmed
Furthermore , we demonstrated that application of
N - propanoylmannosamine leads to a decrease in the polysialylation of the
neural cell adhesion molecule in vivo , which is a marker of poor prognosis for some
tumors with high metastatic potential . -
pubmed
Hemoglobin Constant Spring (
Hb CS ) is an unstable
hemoglobin (
Hb ) variant that results from a
nucleotide substitution at the termination codon of the alpha2 -
globin gene . -
pubmed
Pgp and
MRP activities were measured based on the extrusion of
rhodamine 123 ( for
Pgp ) and
Fluo-3 ( for
MRP ) . -
pubmed
We showed previously that the synthetic peptide P(111 - 136 ) composed of the last 26
HARP amino acids inhibited
HARP - induced mitogenesis . -
pubmed
AgPNP has kcat values of 54 and 41 s-1 for
2 '- deoxyinosine and
inosine , its preferred substrates , and 1.0 s-1 for
guanosine . -
pubmed
This is the tightest - binding inhibitor known for any
PNP , with a remarkable Km / Ki * of 5.4 x 10(7 ) , and is consistent with enzymatic transition state predictions of enhanced transition - state analogue binding in
enzymes with enhanced catalytic efficiency . -
pubmed
Our data show that length - normalized
Hg concentrations ( & gt ; or = 97 % as
MeHg in tissue of both species ) were 230 % greater in
GS in comparison to
RS collected from the same general area . -
pubmed
Stable C and N isotope signatures ( delta15N and
delta13C ) indicate that
GS occupy a slightly higher trophic position ( approximately 30 % of one trophic position higher ) on the Gulf food web in comparison to
RS and that
GS appear to incorporate higher trophic positioned prey , continually and at smaller sizes . -
pubmed
Mercury was strongly correlated with combined
delta15N and
delta13C in pooled species data , arguing that most of the substantial difference in
Hg bioaccumulation between
RS and
GS can be explained by modest differences in their trophic position and , to a lesser degree ,
carbon sources , which had low variation and high overlap among species . -
pubmed
In this study we evaluated the apoptotic response to oxidative stress induced by
2 - deoxy - d - ribose (
dRib ) in peripheral blood lymphocytes ( PBLs ) of two siblings with
Parkinson disease secondary to
A53 T alpha - synuclein mutation . -
pubmed
BACKGROUND : An arteriovenous loop ( AVL ) enclosed in a
polycarbonate chamber in vivo , produces a
fibrin exudate which acts as a provisional matrix for the development of a tissue engineered microcirculatory network . -
pubmed
OBJECTIVES : By administering
enoxaparin sodium - an inhibitor of
fibrin polymerization , the significance of
fibrin scaffold formation on AVL construct size ( including the AVL ,
fibrin scaffold , and new tissue growth into the
fibrin ) , growth , and vascularization were assessed and compared to controls . -
pubmed
Two hours before surgery and 6 hours
post - surgery , saline ( Series I ) or
enoxaparin sodium ( 0.6 mg / kg , Series
II ) was administered intra - peritoneally . -
pubmed
Reductions in the 20 - kDa
myosin regulatory light chain (
MLC20 ) phosphorylation were achieved by inhibiting
MLC kinase with
ML-7 . -
pubmed
Increases in
MLC20 phosphorylation were achieved by inhibiting
myosin light chain phosphatase with
microcystin . -
pubmed
We also found that a heptapeptide that mimics the
actin - binding motif of
myosin II enhanced
microcystin - induced force generation and
actin polymerization without affecting
MLC20 phosphorylation in beta - escin - permeabilized vessels . -
pubmed
Hydrogen peroxide in basic media is proposed as a means for dissolving whole blood samples to be analyzed by electrothermal atomization atomic absorption spectrometry ,
ET AAS . -
pubmed
RESULTS : At
P11 ,
bumetanide ( 0.5 mg / kg ) increased the baseline hippocampal afterdischarge threshold and shortened the afterdischarge duration . -
pubmed
At
P21 ,
bumetanide ( 0.2 ; 2.5 mg / kg ) exerted no effects on hippocampal excitability and kindling progression . -
pubmed
DISCUSSION : The obtained results provide further evidence that
bumetanide may be beneficial for treating neonatal
seizures , and that
NKCC1 represents a potential target for antiepileptic interventions in the immature brain . -
pubmed
Expression systems based on self - cleavable intein domains allow the generation of recombinant proteins with a C - terminal
thioester , providing a unique handle for site - specific conjugation using native chemical ligation (
NCL ) . -
pubmed
The
NMDAR contains a
glycine binding site in its
NR1 subunit that may be a useful target for the treatment of
schizophrenia . -
pubmed
The hypofunctional
Dao 1(G181R ) mutation elevated brain levels of
D - serine , but alone it did not affect performance in the behavioral measures . -
pubmed
Diminished
DAO activity and elevations in
D - serine may serve as an effective therapeutic intervention for the treatment of
psychiatric symptoms . -
pubmed
the
oxygen vacancy occurs at only the
O4 site . -
pubmed
Here , we sought to determine the sensitivity of the manual
Polybrene test in detecting anti -
Jk(a ) and
anti - Jk ( b ) antibodies and
Jk ( a ) and
Jk ( b ) antigens . -
pubmed
Furthermore , 40 randomly selected donor blood samples were collected and phenotyped for the frequencies of
Jk ( a ) and
Jk ( b ) antigens using the manual
Polybrene test and the indirect
antiglobulin test . -
pubmed
RESULTS : The manual
Polybrene test displayed an overall sensitivity of 60 % in detecting
anti - Jk ( a ) and
anti - Jk ( b ) antibody . -
pubmed
Furthermore , the manual
Polybrene test exhibited a sensitivity of 46.15 % for
Jk ( a ) antigen and a sensitivity of 77.42 % for
Jk ( b ) antigen . -
pubmed
CONCLUSION : The manual
Polybrene test has a very low sensitivity in detecting
anti - Jk ( a ) and
anti - Jk ( b ) antibody , especially
anti - Jk ( a ) antibody . -
pubmed
Moreover , the commercial HA ( Shanghai ) could accelerate the oxidation of different
chlorophenols (
CP ) significantly under neutral condition . -
pubmed
The apparent second - order rate constants of 2 -
CP /
phenol oxidation by
permanganate in the presence of HAs correlated well with specific visible absorption ( SVA ) at 665 nm of HAs . -
pubmed
70 %
Ag/30 %
Au was found to be optimum for
SERS excited using 514.5 nm radiation , and it is explained on the basis of composition - dependent changes in the local surface plasmon resonance ( LSPR ) of the electrodeposited
Ag - Au alloy . -
pubmed
RESULTS : In group A ( FO )
LTB4 ,
TXB2 ,
6 - keto prostaglandin F1alpha levels fell during omega-3 administration (
T12 ) . -
pubmed
Bayesian image analysis of
dexamethasone and shear stress - induced
glucocorticoid receptor intracellular movement . -
pubmed
Using a unique expectation - maximization ( EM ) algorithm based on Bayesian statistics , we have developed an image analysis algorithm to quantitatively assess
GR nuclear translocation based on time - lapse images of
green fluorescence protein - tagged
GR (
GFP - GR ) under continuous exposure to a shear stress of 10 or 25 dynes / cm(2 ) as well as to
Dexamethasone , a
GR agonist . -
pubmed
The autoregulation gene
SUNN mediates changes in root organ formation in response to
nitrogen through alteration of shoot - to - root
auxin transport . -
pubmed
RESULTS : We present an analysis of the influence of the tip apex during imaging of the
Si(100 ) substrate in ultra - high vacuum ( UHV ) at 5 K using a qPlus sensor for noncontact atomic force microscopy (
NC -
AFM ) . -
pubmed
For
bright fluorescence emissions , the
carbon nanoparticles may be surface - doped with an inorganic salt and then the same organic functionalization . -
pubmed
Influence of
ABCC2 ,
SLCO1B1 , and
ABCG2 polymorphisms on the pharmacokinetics of
olmesartan . -
pubmed
No significant differences were observed for any other single
nucleotide polymorphism in
ABCC2 ,
SLCO1B1 , or
ABCG2 . -
pubmed
The
ABCC2 -24CC genotype group exhibited lower systemic exposure of
olmesartan than the -24CT genotype group , whereas no significant differences were observed in the other transporter genotype groups . -
pubmed
Collectively , these results demonstrate that
CXCL14 binding to glycoproteins harboring heparan sulfate proteoglycans and
sialic acids leads proliferation and migration of some
cancer cells . -
pubmed
Interactions of
glycine betaine with
proteins : insights from volume and compressibility measurements . -
pubmed
Specifically , we measure the partial molar volumes and adiabatic compressibilities of
cytochrome c ,
ribonuclease A ,
lysozyme , and
ovalbumin in aqueous solutions of the stabilizing osmolyte
glycine betaine ( GB ) at concentrations between 0 and 4 M. The fact that globular proteins do not undergo any conformational transitions in the presence of GB provides an opportunity to study the interactions of
GB with
proteins in their native states within the entire range of experimentally accessible GB concentrations . -
pubmed
Adsorption of
bovine serum albumin (
BSA ) ,
gamma - globulin ,
( 125 ) I -
BSA ,
pepsin , and
chymotrypsin on neat and PEGylated microspheres is determined by UV - VIS spectroscopy of supernatants and eluates or by measurement of radioactivity in an ionization chamber . -
pubmed
While it is thought that bioactivation to reactive
Dcl acyl glucuronides ( AG ) and subsequent protein adduct formation contribute to
Dcl induced
liver injury , LC -
MS /
MS analyses did not reveal increased formation of
4 '- and
5 - hydroxy - Dcl ,
Dcl - AG or
Dcl - AG dependent protein adducts in animals treated with LPS /
Dcl . -
pubmed
Hepatic gene expression analysis suggested enhanced activation of
NFkappaB and
MAPK pathways and up - regulation of co - stimulatory molecules (
IL-1beta ,
TNF - alpha ,
CINC-1 ) by LPS / Dcl and
PIC / Dcl , while protective factors (
HSPs ,
SOD2 ) were down - regulated . -
pubmed
Hyperbranched
polyester - based fluorescent probe for
histone deacetylase via aggregation - induced emission . -
pubmed
Herein , we report a hyperbranched
polyester - based one - step fluorescent assay for
HDAC . -
pubmed
HDAC triggers the deacetylation of
H40 - Lys(Ac ) , thereby turning the electroneutral polymer into the positively charged one . -
pubmed
Phosphorylation of
Thr-457 on the short cytoplasmic domain isoform (
CEACAM1 - SF ) that is predominant in normal epithelial cells is required for lumen formation in a three - dimensional model that involves apoptosis of the central acinar cells . -
pubmed
We conclude that
CaMKIID specifically phosphorylates
Thr-457 on
CEACAM1 - SF , which in turn regulates the process of lumen formation via apoptosis of the central acinar cells . -
pubmed
CONCLUSIONS :
HGF - loaded
PLA - O - CMC nanoparticles can steadily release
HGF , and exhibits better tendencies in liver regeneration , survival rate and hepatic function compared with intravenous
HGF . -
pubmed
In this study , two dimensional ( 2D ) liquid chromatography ( LC ) was coupled in - line with ozonolysis - mass spectrometry (
O3 -
MS ) for the elucidation of
phosphatidylcholine ( PC ) structures in phospholipid extracts . -
pubmed
In
O3 -
MS , PC molecules carried by mobile phase pass through a length of gas - permeable tubing surrounded by
ozone vapor resulting in oxidative cleavage of double bonds . -
pubmed
Thus , by combing the separation power of 2D - LC with in - line
O3 -
MS ( 2D - LC /
O3 -
MS ) , PC species in complex mixtures can be identified including the detailed structure of their two
fatty acid chains . -
pubmed
Among the 15 variables ,
NaNO3 ,
KH2PO4 and
Tween 80 were found as the most significant factors with positive effect on
beta - glucosidase production . -
pubmed
Airway hyperresponsiveness (
AHR ) to
methacholine ( Mch ) , lung histopathology analysis , inflammatory mediators in serum (
IL-4 ,
IL-5 ,
IL-17A ,
IFN - gamma , and
eotaxin ) and in lung (
IL-4 ,
IL-5 ,
IFN - gamma , and
eotaxin ) were selected for investigation and comparison . -
pubmed
The effect of HD in lowering the
IL-4 and
IL-17A level was significantly greater than that of GD ( P & lt ; 0.05 ) , and was not significantly different from
DEX ( P & gt ; 0.05 ) . -
pubmed
HD treatment was more effective in down - regulation of
IL-5 in serum and
eotaxin level both in serum and lung than
DEX ( P & lt ; 0.05 ) . -
pubmed
However , the effect of HD treatment on increase of
IFN - gamma mRNA expression was more apparent than GD and
DEX treatment ( P & lt ; 0.05 ) . -
pubmed
It has been shown the decrease of physical activity , increasing of
corticosterone 's level and gene expression of
cytokines after injection of Poly I : C as a model of
postviral fatigue . -
pubmed
In a second trial , NE was given at 100 ppm alone (
D1 ) or in combination with the prebiotic
PREVIDA ( 0.5 % ) ( PRE ) (
D2 ) for 17 weeks . -
pubmed
In
Alzheimer ' s disease (
AD ) ,
neuritic plaques composed of
beta - amyloid (
Abeta ) deposits and
dystrophic neurites occur frequently around cerebral vasculature , raising a compelling question as to whether , and if so , how ,
microvascular abnormality and
amyloid / neuritic pathology might be causally related . -
pubmed
Analyses of
electrolyte leakage revealed more extensive cell
death in the control plants than in
NbMADS1 - silenced plants . -
pubmed
The compromised stomatal closure observed in the
NbMADS1 - silenced plants was inhibited by the application of
H2O2 and
sodium nitroprusside ( an
NO donor ) . -
pubmed
Design , synthesis , and evaluation of
guanylhydrazones as potential inhibitors or reactivators of
acetylcholinesterase . -
pubmed
Analogs of
pralidoxime , which is a commercial antidote for intoxication from
neurotoxic organophosphorus compounds , were designed , synthesized , characterized , and tested as potential inhibitors or reactivators of
acetylcholinesterase (
AChE ) using the Ellman 's test , nuclear magnetic resonance , and molecular modeling . -
pubmed
The most effective
AChE inhibitor discovered was the
guanylhydrazone derived from
2,4 - dinitrobenzaldehyde and was compared with
tacrine , displaying similar activity to this reference material . -
pubmed
Enhanced
Sodium Ion Storage Behavior of
P2 - Type
Na ( 2 / 3 ) Fe ( 1 / 2 ) Mn ( 1 / 2 ) O2 Synthesized via a Chelating Agent Assisted Route . -
pubmed
In this work ,
P2 - type
Na2/
3Fe1/
2Mn1/
2O2 layered materials were synthesized by a chelating agent assisted sol - gel method with
NH3 H2O. -
pubmed
For long - term cycling , it is preferred to cut off at 4.0 V rather than at 4.3 V. For the optimized
P2 - type
Na2/
3Fe1/
2Mn1/
2O2 calcined at 900 C , a discharge capacity of 92 mAh / g remains after 40 cycles in the voltage range of 1.5 - 4.0 V , and the Coulombic efficiency remains 100 % . -
pubmed
Intravesical Bacillus Calmette - Guerin Versus Combination of
Epirubicin and
Interferon - alpha2a in Reducing Recurrence of
Non - Muscle - invasive Bladder Carcinoma : FinnBladder-6 Study . -
pubmed
INTERVENTIONS : The four - arm study involved a single perioperative instillation of
EPI plus five weekly instillations of BCG or
EPI /
IFN , followed by 11 monthly instillations in the 1 - yr BCG or
EPI /
IFN maintenance arms , further followed by four additional quarterly instillations in the two 2 - yr maintenance arms . -
pubmed
RESULTS AND LIMITATIONS : The median follow - up time was 7.5 and 7.4 yr in the
BCG and
EPI /
IFN groups , respectively . -
pubmed
The probability of
recurrence was significantly lower in the BCG group than in the
EPI /
IFN group . -
pubmed
CONCLUSIONS : The monthly maintenance BCG regimen showed excellent efficacy and was significantly better in preventing
recurrence than a similar regimen of
EPI /
IFN - alpha2a . -
pubmed
PATIENT SUMMARY : A monthly bacillus Calmette - Guerin ( BCG ) regimen showed excellent efficacy and was significantly better in preventing
recurrence than a similar regimen of
epirubicin and
interferon - alpha2a . -
pubmed
Paclitaxel is mainly inactivated in vivo by
cytochrome P5402C8 (
CYP2C8 ) . -
pubmed
In recent years , the
clopidogrel metabolite has been reported to potently inhibit
CYP2C8 . -
pubmed
MEASUREMENTS :
Copeptin , serum
cortisol , 24 - h urinary free
cortisol ,
BMI -
SDS and , as parameters of the
MetS ,
insulin resistance index ( HOMA ) , HbA1c ,
uric acids , blood pressure and lipids . -
pubmed
A 24 - h urinary free
cortisol ( beta - coefficient 0 13 + - 0 06 , P & lt ; 0 001 ) , but not serum
cortisol , was significantly related to
copeptin in multiple linear regression analyses adjusted for age , gender , pubertal stage and
BMI -
SDS . -
pubmed
Further studies are necessary to understand the relationship between
obesity ,
cortisol , gender , pubertal stage and
copeptin levels . -
pubmed
Supt20 interacts with the
Gcn5 - containing
SAGA histone acetylation complex . -
pubmed
In this research , beta - cyclodextrin /
iron oxide reduced
graphene oxide hybrid nanostructure ( beta -
CD / MRGO ) with high water dispersability , excellent magnetic responsivity and molecular selectivity was prepared via a facile one step green strategy . -
pubmed
Given the importance of
ApoA - I in
HDL and
cholesterol transport , it is necessary to understand the mechanisms of
ApoA - I adsorption and the associated structural changes for assessing consequences of ENM exposure . -
pubmed
Here , the authors used a comprehensive array of microscopic and spectroscopic tools to elucidate the interactions between
ApoA - I and 100 nm
Ag nanoparticles ( AgNPs ) with four different surface functional groups . -
pubmed
While the unfolding of
ApoA - I on AgNPs did not significantly change their uptake and short - term
cytotoxicity , the authors observed that it strongly altered the ability of only some AgNPs to generate of reactive
oxygen species . -
pubmed
Spasmolytic Mechanism of Aqueous Licorice Extract on
Oxytocin - Induced Uterine Contraction through Inhibiting the Phosphorylation of
Heat Shock Protein 27 . -
pubmed
The percentage of phosphorylation of
HSP27 at
Ser-15 residue increased up to 50.33 % at 60 min after
oxytocin stimulation . -
pubmed
Colocalization between
HSP27 and
alpha - SMA was observed in the myometrial tissues , especially along the
actin bundles in the
oxytocin - stimulated group . -
pubmed
Additionally , employing ChemGPS -
NP provided support for a preliminary assignment of
liquiritigenin and
isoliquiritigenin as
protein kinase C (
PKC ) inhibitors in addition to
liquiritigenin ,
isoliquiritigenin ,
liquiritin and
isoliquiritin as
MAPK - activated protein kinase 2 (
MK2 ) inhibitors . -
pubmed
Using structural magnetic resonance imaging we examined : i ) volumetric changes and white / grey matter properties of the whole cerebrum and of specific brain areas ; and ii ) whether potential alterations could be normalized by blocking
glucocorticoid receptors with
mifepristone during the critical developmental window of early adolescence , i.e. -
pubmed
METHODS : In this study , 40 Iranian patients were investigated for the presence of the mutations in 6 mitochondrial
tRNA genes ( tRNA
Ile ,
Met ,
Gln ,
Asn ,
Ala and
Trp ) by PCR - SSCP analysis . -
pubmed
[ Detection of
HLA - B * 13:01 gene by dual allele - specific real - time
polymerase chain reaction in patients with
trichlorethylene - induced
dermatitis ] . -
pubmed
Conclusion : Dual allele - specific real - time PCR can be used for detection of the
HLA - B * 13:01 gene in patients with
trichlorethylene - induced
dermatitis . -
pubmed
Clade
IV SWEET proteins were localized to the
tonoplast , and used
hexose as a substrate . -
pubmed
FDA Approval Summary :
Dabrafenib and
Trametinib for the Treatment of Metastatic
Non - Small Cell Lung Cancers Harboring
BRAF V600E Mutations . -
pubmed
IMPLICATIONS FOR PRACTICE : The approvals of
dabrafenib and
trametinib , administered concurrently , provide a new regimen for the treatment of a rare subset of
non - small cell lung cancer (
NSCLC ) and demonstrate how drugs active for treatment of
BRAF - mutant
tumors in one setting predict efficacy and can provide supportive evidence for approval in another setting . -
pubmed
Batatasin III ( 5 ) and
dendrosinen B ( 9 ) showed strong
alpha - glucosidase inhibitory activity . -
pubmed
Dendrosinen B ( 9 ) also exhibited appreciable
pancreatic lipase inhibitory effect . -
pubmed
Organic coated
iron minerals (
iron supplements ) were stable against strong chelators (
calcein and
transferrin ) . -
pubmed
And then the effects of miR-105 were evaluated by performing
trypan blue staining , transwell assay , ANNEXIN -
FITC /
propidium iodide (
PI ) double staining and Western blot analysis . -
pubmed
Besides , inhibition of
mTOR and
p38MAPK pathways by using
Rapamycin and
VX-702 abolished the regulatory effects of
Mcl-1 on EMT . -
pubmed
METHODS : Analyses included adipose mtDNA levels , CI and
CIV activity by immunoassay , visceral adipose tissue by computed tomography , and fasting serum
glucose at week 0 and week 96 of
ART . -
pubmed
CONCLUSIONS : Decreased adipose mitochondrial activity correlated with changes in
adiponectin and
glucose homeostasis on
ART . -
pubmed
In addition , it was revealed that
resveratrol elevated ROS concentration and expression of biomarker of cell
death Bax , while inhibiting
Bcl2 , an anti - apoptotic protein , and reinforcing expression of
p53 . -
pubmed
Moreover ,
resveratrol remarkably increased the expressions of
HIF-1alpha and
p53 in PC cells . -
pubmed
Resveratrol suppressed cell survival and promoted cell
death , but its effects were reversed after
HIF-1alpha knockdown , suggesting that the effects of
resveratrol in PC are mediated via
HIF-1alpha . -
pubmed
CONCLUSIONS Our findings indicate that
resveratrol induces apoptosis via
HIF-1alpha / ROS /
p53 signaling in
prostate cancer cells and may be a useful therapeutic agent against
prostate cancer . -
pubmed
Parathyroid hormone resistance syndromes - Inactivating
PTH /
PTHrP signaling disorders (
iPPSDs ) . -
pubmed
Metabolic disorders caused by impairments of the
Gsalpha /
cAMP /
PKA pathway affecting the signaling of
PTH /
PTHrP lead to features caused by non - responsiveness of target organs , in turn leading to manifestations similar to the
deficiency of the hormone itself . -
pubmed
Serum Lipoprotein Profile Is Associated With Protective Effects of
Oral Contraceptive Use on
Multiple Sclerosis Severity : A Cross - Sectional Study . -
pubmed
A
pyrene - labeled m7
GTP analogue showed up to eightfold enhanced fluorescence emission upon binding to
eukaryotic translation initiation factor 4E (
eIF4E ) and over 30 - fold enhancement upon cleavage by decapping scavenger (
DcpS ) enzyme . -
pubmed
Degradation of
RhB into small organic moieties is detected using LC -
MS technique . -
pubmed
Thioacetamide increased liver weight and serum
alanine aminotransferase and
aspartate aminotransferase levels . -
pubmed
Based on the optimum condition , when the dosage of PS was 5 g / L , the initial concentration of
tetracycline hydrochloride (
TC ) ,
oxytetracycline hydrochloride (
OTC ) and
chlortetracycline hydrochloride (
CTC ) was 250 mg / L , the removal rate of PS was 55.90 % , 45.10 % and 14.98 % higher than those of EC process after 40 min treatment . -
pubmed
Herein , we adopt an alternative strategy , where the bistable ferroelectric switches ( P and P ) of
Sc2CO2 ( SCO ) assist the ferromagnetic states of
Cr2Ge2Te6 (
CGT ) in order to achieve non - volatile memories . -
pubmed
Moreover ,
MXene SCO , being an aided layer in multiferroic
CGT /
SCO hetero - structures , also modifies the electronic properties of
CGT to half metal by its polarized P state . -
pubmed
The plasma concentrations of
LH ,
FSH and
testosterone were low at the beginning ; each short infusion of
LH - RH resulted in a transitory increase in the level of
LH and
testosterone while the concentration of
FSH was only marginally affected . -
pubmed
The response to the individual injections of
LH - RH was also modified , the peak in
LH being reduced in amplitude but more prolonged while the
FSH and
testosterone responses were both enhanced . -
pubmed
When the pulsed infusion was stopped the concentration of
LH and
testosterone declined rapidly while the decline in
FSH levels took many days . -
pubmed
Plasmic digestion of
fibrinogen yielding fragment X , did not reduce the accelerating affect on erythrocyte sedimentation , indicating that the intact
carboxyl terminal end of the
A alpha chain is unnecessary for this phenomenon . -
pubmed
Lactate dehydrogenase isoenzymes of 10 museum
strains of lactobacilli were examined electrophoretically in
polyacrylamide gel . -
pubmed
The stimulus - secretion coupling of
glucose - induced
insulin release - XVII . -
pubmed
The usefulness of these adaptations is illustrated by their application to the simultaneous detection of three point mutations , two in the
tyrosine kinase domain of the
insulin receptor and one in the
insulin - responsive glucose transporter (
GLUT4 ) in a highly
insulin - resistant
NIDDM population . -
pubmed
As an extension of previous investigations on synthesis and
dopamine autoreceptor activity of
bicyclic ergoline analogues the
tricyclic azaergoline analogues 9a and 9b were synthesized . -
pubmed
Using the
SH group cross - linker
phenylarsine oxide as the inducer , we show that both matrix pH and the membrane potential can modulate the process of
PTP induction independently of
Ca2 + . -
pubmed
matrix alkalinization and membrane depolarization ) , we have explored the possibility that the
Ca ( 2 +)-induced changes of the delta mu H+ may contribute to
PTP induction by
Ca2 + . -
pubmed
Our data in mitochondria treated with
Ca2 + plus
N - ethylmaleimide and
Ca2 + plus
phosphate show that membrane depolarization is a powerful inducer of the
PTP . -
pubmed
Taken together , our observations indicate that the
PTP can be controlled directly by the delta mu H+ both in the absence and presence of
Ca2 + , and suggest that a collapse of the membrane potential may be the cause rather than the consequence of
PTP induction under many experimental conditions . -
pubmed
A new type of
chloride channel has been identified by functional expression of
phospholemman , a 72 -
amino acid cardiac sarcolemmal protein with a single transmembrane domain . -
pubmed
SCATyr ( Me ) AVP elicited outstanding antagonistic properties by blocking at concentration of 10(-7 ) M nearly completely the constrictory activity of
AVP . -
pubmed
Second , complementation analysis indicates that high - level expression of both the
gltD and
gltF genes is required for the induction of the
Ntr enzymes under
nitrogen - limiting conditions , indicating that the phenotype of the
gltB insertion probably results from polarity on
gltD and
gltF. -
pubmed
Evaluation of
LH and
FSH secretion during a 24 - h period was performed in a group of 15 PMW after a single oral dose of 600 or 1,000 mg of
ipriflavone or placebo , and after 7 , 14 and 21 days of oral treatment with
ipriflavone 600 mg and 1,000 mg / daily , administered in three divided doses . -
pubmed
LH secretion was also evaluated during
naloxone infusion before and after 21 days of
ipriflavone , placebo or conjugated
estrogen treatment ( 0.625 mg / day ; CE ) . -
pubmed
LH response to
NAL treatment was absent during
ipriflavone and placebo such as it was observed before treatments . -
pubmed
DESIGN : A prospective randomized trial was set up to compare long and short regimens of
gonadotropin - releasing hormone agonist administration for ovulation induction in
IVF . -
pubmed
Furthermore , serum
alpha - tocopherol correlated with
retinol ( r = 0.53 , P less than 0.001 ) ,
selenium ( r = 0.45 , P less than 0.001 ) , and
albumin ( r = 0.37 , P less than 0.001 ) in serum . -
pubmed
Activation involves tryptic cleavage of an N - terminal pentapeptide
Ala - Pro - Gly - Pro - Arg ( APGPR ) which is known as
procolipase activation peptide (
CLAP ) . -
pubmed
This loss of reactivity can be significantly slowed by the addition of 20 mmol / l
Zinc ions (
Zn2 + ) , while
ethylenediaminetetra - acetic acid (
EDTA ) and other
protease inhibitors were ineffective . -
pubmed
Additionally the increased blood flow may be associated with the previous findings of increased synthesis of
prostaglandins , gastric mucus and
bicarbonate secretion by
CBS . -
pubmed
The wild - type and mutant
PDH complexes were purified to near - homogeneity and were analyzed by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis , immunoblotting , and enzyme assays . -
pubmed
Deletion of most of the putative
lipoyl domain ( residues 8 - 84 ) resulted in loss of about 85 % of the overall activity , but did not affect the
acetyltransferase activity of
E2 or the binding of
pyruvate dehydrogenase (
E1 ) ,
dihydrolipoamide dehydrogenase (
E3 ) , and
protein X to the truncated
E2 . -
pubmed
Although the
lipoyl domain of
E2 and/or its covalently bound
lipoyl moiety were removed , the mutant complexes retained 12 - 15 % of the overall activity of the wild - type
PDH complex . -
pubmed
Replacement of both
lysine-47 in
E2 and the equivalent
lysine-43 in
protein X by
arginine resulted in complete loss of overall activity of the mutant
PDH complex. -
pubmed
The cell adhesive proteins
collagen and
fibronectin , and the cell adhesive oligopeptide RGDS (
arginine -
glycine -
aspartic acid -
serine ) were successfully covalently immobilized on
poly ( vinyl alcohol ) (
PVA ) hydrogel sheets . -
pubmed
Regulation of
protein kinase C activity by
gangliosides . -
pubmed
The activity of
protein kinase C (
Ca2 +/ phospholipid - dependent enzyme ) in the presence of
phosphatidylserine and its physiological regulator ,
diacylglycerol , could be suppressed by a mixture of brain
gangliosides . -
pubmed
Inhibition was observed at all concentrations of
Ca2 + between 10(-8 ) and 10(-4 ) M. Inhibition of
protein kinase C activity could not be reversed by increasing the concentration of
diacylglycerol or the substrate , histone . -
pubmed
Our results suggest that
gangliosides may regulate the responsiveness of
protein kinase C to
diacylglycerol . -
pubmed
The properties of transcellular
K + movement can be adequately described by a model which provides for active
K + uptake across the basolateral membrane via an electrogenic
Na / K ATPase and K+ exit from the cell across the apical membrane down an electrochemical potential gradient via K+ channels which are blocked by mucosal
barium . -
pubmed
Paracellular biliary access of anionic
horseradish peroxidase was less than 50 % that of neutral and cationic
horseradish peroxidase both in the control state and when paracellular entry was augmented with 10(-10 ) M
vasopressin . -
pubmed
In order to predict a
haemoglobin (
Hb ) rise , in response to treatment with
iron from simple erythrocyte and serological parameters , we treated 28 anaemic
RA patients with oral
iron during 6 weeks . -
pubmed
Iron treatment is only useful in
iron deficient
RA patients , although active
RA limits maximal
Hb rise . -
pubmed
Pretreatment of these cells with 500ng / ml of
pertussis toxin for 24h prior to addition of
5HT reduced
5HT - induced formation of
inositol phosphates . -
pubmed
These data suggest that a
guanine nucleotide inhibitory ( Gi ) protein couples
5HT receptor to
phospholipase C and
TPA modulates
5HT - stimulated hydrolysis of
inositol phospholipids in cultured VSMC through activation of
PKC . -
pubmed
In contrast , the activities of
lactate and
malate dehydrogenases were unchanged , and that of
acetylcholinesterase apparently increased . -
pubmed
Thus satisfactory histochemical demonstration of all enzymes was achieved ( except with
alkaline phosphatase ,
lactate and
malate dehydrogenases ) following the microwave - saline pretreatment . -
pubmed
For this purpose a structural analysis was employed and blood levels of
gonadotrophins and
testosterone were radioimmunoassayed . -
pubmed
Glucosidase II is an endoplasmic - reticulum - localized enzyme that cleaves the two internally alpha-1,3 - linked
glucosyl residues of the
oligosaccharide Glc alpha 1 - --- 2Glc alpha 1 - --- 3Glc alpha 1 - --- 3Man5 - 9GlcNAc2 during the biosynthesis of
asparagine - linked glycoproteins . -
pubmed
The thyroid
protein kinase C was not stimulated by
Ca2 + or
phosphatidylserine (
PS ) , but was stimulated by the combination of the two by 570 % . -
pubmed
Diolein stimulated the
kinase by increasing its sensitivity to
Ca2 + . -
pubmed
Other
phospholipids could not substitute for
PS and were ineffective in stimulating the
protein kinase C in the absence of
diolein . -
pubmed
Quercitin , a
protein kinase C inhibitor in other systems , inhibited the thyroid enzyme in a dose - related manner . -
pubmed
Separation of these
32P - labelled proteins by electrophoresis and subsequent autoradiography revealed that three proteins were phosphorylated by the
protein kinase C of approximate molecular weights 60,000 , 45,000 , and less than 29,000 . -
pubmed
DCBT irreversibly inhibits in vitro polymerization of purified
tubulin , but only after a prolonged preincubation of the protein with the drug . -
pubmed
Binding of radiolabeled
DCBT to
tubulin similarly requires a long incubation time , with the reaction not being complete even after 6 hr at 37 degrees C. A specific interaction with
tubulin is also shown by the crossresistance to
DCBT of
Colcemid - resistant cells with an altered
beta - tubulin . -
pubmed
Both
strains were , however , resistant to relatively high concentrations of reagent
H2O2 or
H2O2 enzymatically generated by
glucose and
glucose oxidase ,
KI , or
lactoperoxidase (
LPO ) alone . -
pubmed
Kinetic studies using small quantities of reagent or enzymatically generated
H2O2 and
LPO - KI showed that the system was lethal for both fungal
strains within 1 min . -
pubmed
Furthermore , using the
glucose - glucose oxidase -
LPO - KI system , it was shown that
catalase ,
superoxide dismutase and
histidine scavengers of
H2O2 ,
superoxide anion and singlet
oxygen , respectively , prevented the killing of fungus , whereas scavengers of
hydroxyl radicals such as
benzoate and
mannitol had no effect . -
pubmed
Consequently , the
xanthine ( or
hypoxanthine ) and
xanthine oxidase system in which the main product is
superoxide anion had no toxic effect on the fungus . -
pubmed
The high sensitivity of dermatophytes to killing by the
H2O2 - peroxidase -
halide system active in polymorphonuclear neutrophils and macrophages may account in part for
fungal toxicity in vivo . -
pubmed
A nonhistone protein , which migrates between oxidized histone
H3 and histone
H1 has been found to be increased in amount following
cycloheximide treatment . -
pubmed
Lys- and Glu -
plasminogen caused 50 % inhibition of anti -
kringle 4 binding to
125I - kringle 4 at ratios of 4 and 20 mol / mol ( competitor / kringle 4 ) , respectively . -
pubmed
Amyloid beta - protein activates
tachykinin receptors and
inositol trisphosphate accumulation by synergy with
glutamate . -
pubmed
ABP weakly activated
tachykinin receptors , but in the presence of
N - methyl - D - aspartate and alpha - amino-3 - hydroxy-5 - methylisoxazole-4 - propionate - type glutamate receptors ABP - induced responses were greatly enhanced . -
pubmed
These observations suggest that in the presence of
glutamate ,
ABP can activate
tachykinin receptors and
phosphatidylinositol turnover . -
pubmed
A panel of 12 neutralizing anti -
LtxA monoclonal antibodies also recognized specificities within the 253 -
amino acid fragment . -
pubmed
Prompted by the reversal of
skin hyperproliferation to normal by
13 - hydroxyoctadecadienoic acid (
13 - HODE ) , a
15 - lipoxygenase metabolite of
linoleic acid , we investigated a possible mechanism for this antiproliferative action . -
pubmed
To address this we first demonstrated that
13 - HODE is incorporated into epidermal
phosphatidyl 4,5 - bisphosphate (
PtdIns4,5 - P2 ) and released as
13 - HODE - containing
diacylglycerol by
epidermal phospholipase C. -
pubmed
Secondly , we tested the possibility whether this putative
13 - HODE - containing
DAG (
13HODE - DAG ) could exert a modulatory effect on epidermal
protein kinase C (
PKC ) activity which previously has been associated with
skin hyperproliferation . -
pubmed
Unlabeled
13HODE - DAG was generated from
13 - HODE - containing
phosphatidylcholine after
phospholipase C hydrolytic cleavage . -
pubmed
The effects of the
13HODE - DAG were determined on : i ) total epidermal
PKC activity ; ii )
diolein - activated
PKC activity ; and iii ) the two identified
epidermal PKC - isozymes (
PKC - beta and
PKC - alpha ) . -
pubmed
Our data revealed over a twofold activation of total basal
PKC activity by
diolein . -
pubmed
In contrast , replacement of
diolein (
1,2 - dioleoylglycerol ) with
13HODE - DAG (
1 - palmitoyl,2 - 13HODE - glycerol ) in the incubation mixture exerted no effect on total basal
PKC activity . -
pubmed
In an another experiment ,
13HODE - DAG inhibited
diolein - activated
PKC activity in a dose - dependent manner . -
pubmed
To determine whether the effects of
13HODE - DAG are selective , we tested its effects on
DEAE - Sephacel - purified and Western blot - confirmed
PKC isozymes . -
pubmed
Our data revealed that
13HODE - DAG selectively inhibited the activity of
PKC - beta isozyme , while exerting negligible effect on the
PKC - alpha isozyme . -
pubmed
This selective inhibitory effect of
13HODE - DAG on a major epidermal
PKC isozyme activity suggests that
13HODE - containing
DAG seemingly can modulate epidermal
PKC activity , which purportedly is associated with epidermal hyperproliferation . -
pubmed
DNA sequence analysis of this clone , which was designated as
Oryza sativa homeobox 1 (
OSH1 ) , and a genomic clone encoding the
OSH1 sequence have shown that the
OSH1 gene consists of five exons and encodes a polypeptide of 361
amino acid residues . -
pubmed
The effects of two months of
streptozotocin - induced
diabetes and
aldose reductase inhibition on
body weight , plasma
glucose , and nerve
sugars and
polyols were comparable to those reported previously . -
pubmed
Thus , either deficits in laser Doppler blood flow and conduction are not
aldose reductase inhibitor - dependent or
tolrestat has some other property which offsets the beneficial effects of
aldose reductase inhibition . -
pubmed
Laboratory studies revealed increased total
thyroxine ( T4 ) and
triiodothyronine ( T3 ) concentrations , a high - normal free T4 concentration , and a normal basal
thyrotropin (
TSH ) concentration with a normal response to
thyroliberin (
TRH ) . -
pubmed
Immunoelectrophoresis in the presence of
[ 125I ] T4 or
[ 125I ] T3 localized the
radiolabeled iodothyronines to a band corresponding exactly to the precipitin arc of the monoclonal
IgA. -
pubmed
Purified
IgA ( 50 micrograms ) bound both
[ 125I ] T4 ( 12.3 % ) and
[ 125I ] T3 ( 2.7 % ) specifically and in a dose - dependent manner . -
pubmed
Scatchard analysis of competitive - binding data utilizing
[ 125I ] T4 and unlabeled T4 revealed a Kd of 2.2 x 10(-7 ) mol / L. The binding capacity for T4 was approximately 7 mumol / L. Thus , in this case of
IgA - secreting
myeloma , the monoclonal
IgA acts as an additional
thyroid hormone - binding protein in serum that interferes in the T4 and T3 RIAs . -
pubmed
Despite the unusual specificity of the
RNase H activity of
reverse transcriptase , which cleaves the RNA c - a
phosphodiester rather than the junction a - C linkage , we found no major structural differences among the RNA c - a
phosphodiester rather than the junction a - C linkage , we found no major structural differences among the RNA
sugar conformations -- all RNA
sugars were found in the normal C3'-endo A - form conformation . -
pubmed
The
norepinephrine transporter (
NET ) is a site of action for tricyclic antidepressant drugs and for drugs of abuse such as
amphetamine and
cocaine . -
pubmed
Various membrane markers including
lipooligosaccharide ( LOS ) , the 16 - kDa outer membrane lipoprotein (
SmpA ) , and the C subunit of the
F1F0 ATPase indicated that the lowest - density membrane fraction contained outer membranes while the high - density membrane fraction contained inner membranes and that both are essentially free of contamination by the periplasmic flagella , a major contaminant of membranes isolated by other techniques . -
pubmed
Multiple alignments of these
Vg sequences indicate that the variation in cleavage location has not arisen through exon shuffling , but through local modifications of the
amino acid sequences . -
pubmed
The so - called
epidermal growth factor precursor region contains two types of motifs (
cysteine - rich Class B repeats and YWXD repeats ) which occur independently of one another in diverse proteins , and are often involved in protein - protein interactions , suggesting that they potentially are involved in dimerization of
VgRs and other
LDLR - family proteins . -
pubmed
Pancreatic polypeptide (
PP ) is a member of a family of
36 - amino acid brain - gut peptides , including
neuropeptide Y ( NPY ) and
polypeptide YY (
PYY ) and acting through many subtypes of
Y receptors belonging to the superfamily of the
G protein - coupled receptors . -
pubmed
Load - dependent mechanical efficiency of individual
myosin heads in skeletal muscle fibers activated by laser flash photolysis of caged
calcium in the presence of a limited amount of
ATP . -
pubmed
Immediately before laser flash activation , almost all of the
myosin heads in the fiber are in the state , M.ADP.Pi , and can undergo only one
ATP hydrolysis cycle after activation . -
pubmed
( + ) - Cis-3,5 - dimethyl-2 -( 3 - pyridyl ) thiazolidin-4 - one hydrochloride (
SM-12502 ) is a newly developed drug as a
platelet - activating factor receptor antagonist . -
pubmed
A new RFLP characterized by
SacI and SphI was found to be due to the entire gene deletion of the three exons and was associated with the decreased metabolism of
SM-12502 . -
pubmed
On the other hand mast cells play their role in defence of peritoneal cavity via
TNF - alpha and
histamine release and inhibition of fibrynolysis . -
pubmed
Reply :
cytochrome P450 - derived
eicosanoids and the vascular wall . -
pubmed
These data were confirmed by the
Tdt - mediated d ' UTP -
FITC nick end labeling positivity of the cells and the fragmentation of the nucleus visualized by
4 ' , 6 - diamidino-2 - phenylindole and DNA ladder techniques . -
pubmed
Trio is a complex protein containing two
guanine nucleotide exchange factor domains each with associated pleckstrin homology domains , a
serine /
threonine kinase domain , two SH3 domains , an
immunoglobulin - like domain , and spectrin - like repeats . -
pubmed
RhoA isoprenylation is necessary for the
RhoA -
Trio interaction , because mutation of the
RhoA carboxyl - terminal
cysteine residue blocked binding . -
pubmed
METHODS : After extraction , chromatographic separation was achieved on a narrow - bore
C ( 18 ) column by gradient elution with fluorometric detection ; results were confirmed by liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) . -
pubmed
During chronic osmotic stimulation by giving 2 %
NaCl solution for 2 and 5 days , a large number of
Fos - positive neurons were observed , but the cessation of chronic osmotic stimulation by normal water drinking immediately decreased the number of
Fos - positive neurons to the control level within 2 h. The number of
FosB - positive neurons was increased with period of chronic osmotic stimulation , and a significant number were observed 2 - 8 h after the cessation of the stimulation . -
pubmed
The diet rich in
MCT significantly raised the plasma level of
triacylglycerols when compared to either the starch- or cellulose - rich diet . -
pubmed
Postprandial
insulin concentrations were lowest for the
MCT diet , and concentrations of free
fatty acids were highest . -
pubmed
Endogenous
methylarginines regulate
neuronal nitric - oxide synthase and prevent
excitotoxic injury . -
pubmed
While
guanidino - methylated arginines ( MA ) including
asymmetric dimethylarginine (
ADMA ) and
N ( G )- methyl - l - arginine (
NMA ) are potent competitive inhibitors of
nitric oxide synthase (
NOS ) and are released upon protein degradation , it is unknown whether their intracellular concentrations are sufficient to critically regulate neuronal
NO production and secondary cellular function or
injury . -
pubmed
Therefore , we determine the intrinsic neuronal
MA concentrations and their effects on neuronal
NOS function and
excitotoxic injury . -
pubmed
Normal neuronal concentrations of all
NOS - inhibiting MA were determined to be approximately 15 microm , while
l - arginine concentration is approximately 90 microm . -
pubmed
These MA levels result in & gt;50 % inhibition of
NO generation from
neuronal NOS . -
pubmed
Thus , neuronal MA profoundly modulate
NOS function and suppress
NO mediated
injury . -
pubmed
GSNO ( 1,000 microM ) caused inhibition of
collagen and
ristocetin induced aggregation by approximately 50 % . -
pubmed
The
Coomassie blue staining intensity of all
crystallin 2D gel components was 2- to 3 - fold greater in light exposed than in control retinas . -
pubmed
Oxygen ,
beta2 agonists , and systemic
corticosteroids are the mainstays of acute
asthma therapy . -
pubmed
FK506 inhibits
Erk and
PI-3K /
Akt signaling , two crucial pro - survival pathways . -
pubmed
The levels of phosphorylated
Akt and
p42 / 44 Erk decline in few hours after
FK506 addition . -
pubmed
Interestingly , expression of
BDNF increased in a dose - dependent manner in
FK506 - treated astrocytes . -
pubmed
Upregulation of
BDNF mRNA and protein level in astrocytes exposed to
FK506 may underlie neuroprotective action of
FK506 . -
pubmed
Structure - function study of the
amino - terminal stretch of the
catalase subunit molecule in oligomerization ,
heme binding , and activity expression . -
pubmed
CTCDelta1 - 4 and
CTCDelta1 - 24 , whose N - terminal regions were shortened by four and 24
amino - acid residues , respectively , showed an 80 % decrease in specific activity compared to wild - type
CTC in spite of containing the same amount of
heme as in the wild - type . -
pubmed
Although the smaller oligomers were found to be bound with
heme , only the tetramer exhibited
catalase activity in activity staining on nondenaturing gel . -
pubmed
CTCDelta1 - 49 , a mutant with deletion of the N - terminal 49
amino - acid residues which contain the conserved helix alpha2 , showed no
catalase activity and no
heme binding . -
pubmed
However , the CD spectrum profiles of
CTCDelta1 - 49 ,
CTCDelta1 - 4 , and
CTCDelta1 - 24 indicated that these mutant subunits could attain secondary conformations similar to that of wild - type
CTC , regardless of their binding with
heme . -
pubmed
From these results , it was concluded that the N - terminal stretch of
catalase is significant for complete assembly into active tetramer and that the conserved helix alpha2 , although it has little effect on the formation of the subunit secondary structure , is indispensable not only in assembling tetramer but also in binding
heme . -
pubmed
To regulate the production of proinflammatory compounds , a specific peptide inhibitor
Val - Ala - Phe - Arg - Ser ( VAFRS ) for the
group I PLA ( 2 ) enzymes has been designed and synthesized . -
pubmed
Cyclin D1 mRNA and protein expressions were significantly lower in the PVL than in the PVNL and
HEP at 18 h. The levels of mRNA and protein expression of
cyclin A were significantly lower in the PVL than in the PVNL and
HEP at 36 h. Liver regeneration , assessed by the relative liver weight ,
thymidine incorporation into DNA , and
proliferating cell nuclear antigen (
PCNA ) labeling index was delayed significantly in the PVNL compared to that in the
HEP . -
pubmed
A rise in
D - glucose concentration from 3.3 to 5.0 or 7.3 mM or the administration of
D - fructose ( 17 and 40 mM ) in the presence of 3.3 mM
D - glucose stimulated
insulin release in a concentration - related manner , but failed to affect
somatostatin output . -
pubmed
The secretory response to
L - arginine ( 5 mM ) , 25 min after restoring the basal concentration of
D - glucose , was more markedly affected , in terms of potentiation of
insulin and
somatostatin release and reduction of
glucagon output , after prior administration of
D - fructose than after a prior increase in
D - glucose concentration . -
pubmed
A combination of selective
COX-2 inhibitors and
hydrogen peroxide increase the reactive
oxygen species formation in
osteosarcoma cells after X - ray irradiation . -
pubmed
We investigated whether a combination of selective
COX-2 inhibitors and
hydrogen peroxide increases the effect of X - ray irradiation , with regard to reactive
oxygen species ( ROS ) formation in an
osteosarcoma cell line . -
pubmed
A low dose concentration of
COX-2 inhibitor in combination with
hydrogen peroxide and irradiation did affect ROS formation in the intracellular compartment ; however , this same combination of agents at high doses did not modulate the effect of irradiation . -
pubmed
Therefore , low doses of
COX-2 inhibitor and
hydrogen peroxide together , in combination with irradiation , is a potentially useful alternative form of radiotherapy for apoptotic - resistant
neoplasms such as
osteosarcoma . -
pubmed
Regardless of which mechanism is correct , the selective
toxicity of
chloroquine for
malaria parasites is probably due to
permease - mediated uptake . -
pubmed
Therefore we analyzed serum oxdative stress marker (
8 - OHdG ) and anti oxidative substances (
Mn - SOD and
TRX ) to evaluate their clinical significance . -
pubmed
Though there was no relation between serum level of
8 - OHdG and clinical data , positive correlation between serum level of
Mn - SOD ,
TRX and serum level of
ALP ,
IgM was observed . -
pubmed
It also made clear that genotype of
GST related to the titer of
AMA . -
pubmed
RATIONALE :
Glutamate , the major excitatory neurotransmitter in the brain mediates its effects by both
ionotropic and metabotropic receptor subtypes . -
pubmed
Recently , the search for selective ligands for
glutamate receptor subtypes has led to the discovery of
2 - methyl-6 -( phenylethynyl ) pyridine (
MPEP ) , an antagonist specific for
metabotropic glutamate receptor 5 (
mGlu5 ) . -
pubmed
OBJECTIVES : We sought to assess the effects of
mGlu5 receptor antagonists ,
MPEP and
3 -[( 2 - methyl-1,3 - thiazol-4 - yl ) ethynyl ] pyridine (
MTEP ) on cortical
norepinephrine ( NE ) levels . -
pubmed
CONCLUSIONS : Taken together , these data show that
MPEP and
MTEP , through their blockade of the
mGlu5 , reduce extracellular
norepinephrine , the impact of which may contribute to their anxiolytic actions . -
pubmed
Sarco / endoplasmic reticulum Ca ( 2 +) ATPases (
SERCAs ) pump free
Ca ( 2 + ) from the cytosol into the endoplasmic reticulum . -
pubmed
However , doubly transgenic males ( HCM /
GSK-3beta ) demonstrated depressed contractile function , reduced sarcoplasmic ( endo ) reticulum
Ca(2+)-ATPase (
SERCA ) expression , elevated
atrial natriuretic factor (
ANF ) expression , and
premature death . -
pubmed
Role of
TGF - beta and
PGE2 in T cell responses during
Plasmodium yoelii infection . -
pubmed
We found that during
acute infection DC migrate to the spleen and secrete
TGF - beta ,
prostaglandin E2 (
PGE2 ) and
IL-10 . -
pubmed
During blood - stage
infection , inhibition of the activity of
TGF - beta and
PGE2 restores the
CD8 + T cell responses generated by sporozoites , increasing protection against
re - infection . -
pubmed
Sphingosine-1 - phosphate ( SIP ) receptor 1 and the circulatory lipid ,
S1P , are required for lymphocyte egress from lymphoid organs . -
pubmed
Among its various pharmacological actions ,
TPM has been shown to inhibit the activity of specific
carbonic anhydrase enzymes in the kidney . -
pubmed
The increased levels of
Cu and
Fe in
CSF may indicate an active role of these metals in the pathogenesis of
Skogholt ' s disease . -
pubmed
However , no association was found between genetic factors and
Pgp function using
rhodamine 123 accumulation . -
pubmed
The putative
Mn ( II ) oxidase genes were not among the predicted targets , indicating that regulation of
Mn ( II ) oxidation is controlled by other factors yet to be identified . -
pubmed
Nqo1 mRNA , protein , and activity levels were determined through 96 h in
SD and ACI males and females following treatment with known
Nqo1 inducers
oltipraz and
butylated hydroxyanisole . -
pubmed
The
ABC transporter AtABCB14 is a
malate importer and modulates stomatal response to
CO2 . -
pubmed
Here we show that the ABC transporter
AtABCB14 modulates stomatal closure on transition to elevated
CO2 . -
pubmed
Stomatal closure induced by high
CO2 levels was accelerated in plants lacking
AtABCB14 . -
pubmed
Apoplastic
malate has been suggested to be one of the factors mediating the stomatal response to
CO2 ( Refs 4,5 ) and indeed , exogenously applied
malate induced a similar
AtABCB14 - dependent response as high
CO2 levels . -
pubmed
In isolated epidermal strips that contained only guard cells ,
malate - dependent stomatal closure was faster in plants lacking the
AtABCB14 and slower in
AtABCB14 - overexpressing plants , than in wild - type plants , indicating that
AtABCB14 catalyses the transport of
malate from the apoplast into guard cells . -
pubmed
We therefore suggest that
AtABCB14 modulates stomatal movement by transporting
malate from the apoplast into guard cells , thereby increasing their osmotic pressure . -
pubmed
Positive affect was inversely related to the increase in
cortisol 30 min
post awakening ( p=.042 ) , and unrelated to the evening decline in
cortisol levels ( p=.174 ) . -
pubmed
We have examined three methods for determining the local apparent contact potential difference (
CPD ): frequency modulation KPFM ( FM - KPFM ) , amplitude modulation KPFM (
AM - KPFM ) , and frequency shift - bias spectroscopy , on a test system of
3,4,9,10 - perylene tetracarboxylic dianhydride (
PTCDA ) on
NaCl , an example of an organic semiconductor on a bulk insulating substrate . -
pubmed
We will also discuss the distance dependence of the
CPD as measured by FM - KPFM for both the
PTCDA organic deposit and the
NaCl substrate . -
pubmed
CONCLUSION : Unlike
ADC ,
( 23 ) Na MRI detected within - core
ischemic lesion heterogeneity . -
pubmed
The
tyrosine kinase Pyk2 plays a unique role in intracellular signal transduction by linking
Ca ( 2 + ) influx to
tyrosine phosphorylation , but the molecular mechanism of
Pyk2 activation is unknown . -
pubmed
In neurons ,
Ca ( 2 + ) influx through
NMDA - type glutamate receptors causes postsynaptic clustering and autophosphorylation of endogenous
Pyk2 via
Ca ( 2 +)- and
calmodulin - stimulated binding to
PSD-95 . -
pubmed
Accordingly ,
Ca ( 2 + ) influx promotes oligomerization and thereby autoactivation of
Pyk2 by stimulating its interaction with
PSD-95 . -
pubmed
Platelets were stimulated with 75 muM
arachidonic acid ( AA ) , 10 muM
ADP or 25 muM
TRAP . -
pubmed
After stimulation with each agonist , a greater proportion of large platelets bound
fibrinogen ,
VWF ,
P - selectin and activated
integrin alphaIIbbeta3 than small platelets both in the presence and in the absence of in vitro
aspirin . -
pubmed
The prepared samples were characterized by FTIR ,
TG , SEM , and
N ( 2 ) and
CO ( 2 ) adsorption . -
pubmed
We incorporated simulations of the pathways that translate an increase in [
Ca](cyt ) to the activation of
myosin light chain (
MLC ) kinase and cell contraction , as well as the kinetics of
NO and reactive
oxygen species formation and their effects on [
Ca](cyt ) and MLC phosphorylation . -
pubmed
This results from stimulation of
Ca ( 2 + ) uptake from the cytosol into the
SR via
SERCA pumps ,
Ca ( 2 + ) efflux into the extracellular space via plasma membrane
Ca ( 2 + ) pumps , and
MLC phosphatase (
MLCP ) activity . -
pubmed
At concentrations above 1 nM ,
O ( 2 )(- ) is predicted to modulate [
Ca(cyt ) ] and
MCLP activity mostly by reducing
NO bioavailability . -
pubmed
The DVR vasoconstriction that is induced by high concentrations of
H ( 2 ) O ( 2 ) can be explained by
H ( 2 ) O ( 2 )-mediated downregulation of
MLCP and
SERCA activity . -
pubmed
We conclude that intrinsic generation of
NO by the DVR wall may be sufficient to inhibit vasoconstriction by maintaining suppression of
MLC phosphorylation . -
pubmed
In this article , the photo - degradation of
4 - chlorophenol ( 4 -
CP ) under UV irradiation was studied with focus on the photodecomposition of
4 - CP by reactive
oxygen species ( ROS ) . -
pubmed
4 -
CP underwent much faster and more complete degradation in UV / air system than in UV /
N ( 2 ) system . -
pubmed
Angiotensin II upregulation of cardiomyocyte
adiponectin production is
nitric oxide /
cyclic GMP dependent . -
pubmed
A
nitric oxide synthase inhibitor (
Nx - nitro - l - arginine methyl ester hydrochloride ) and an analog of
cGMP antagonist (
Rp-8 - Br - CGMP - S ) blocked
Ang II - mediated upregulation of
adiponectin . -
pubmed
CONCLUSIONS : These data suggest a mechanism whereby
Ang II upregulates
adiponectin in NRVMs via the
angiotensin type-2 receptor /
nitric oxide /
cGMP/
protein kinase G signaling pathway . -
pubmed
HBO was administered at a pressure of 3 atmospheres absolute for 1 h with 100 %
oxygen , starting at 3 h
post brain ischemia in
HBO groups . -
pubmed
The
infarct volume (
IV ) , activation of astrocyte , and level of total
glutathione and lipid peroxidation (
LP ) were assessed 24 h
post - reperfusion . -
pubmed
Significant reduction in
IV was noted in
HBO group when compared with control group . -
pubmed
The Paterno - Buchi (
PB ) reaction of
geraniol derivatives 1 , which contain
allylic alcohol functionality and unfunctionalized double bonds , with
benzophenone was investigated to see the effect of the
hydroxyl group on the regioselectivity of the
oxetane formation , i.e. -
pubmed
The
oxetane 2a is derived from the
PB reaction at the
allylic alcohol moiety , whereas the
PB reaction at the unfunctionalized double bond produces the
oxetane 3a . -
pubmed
The
PB reaction of the
hydroxy - protected
methyl ether 1b and
acetate 1c gave selectively
oxetanes 3b , c derived from the reaction at the more nucleophilic double bond , 2/3~ 15/85 . -
pubmed
The
hydroxyl - group effect was found to be small , but apparently increased the formation of 2a in the
PB reaction with
geraniol ( 1a ) . -
pubmed
Killing of
chronic lymphocytic leukemia by the combination of
fludarabine and
oxaliplatin is dependent on the activity of
XPF endonuclease . -
pubmed
We postulated that a mechanism for this activity is the incorporation of
fludarabine into DNA during
nucleotide excision repair (
NER ) stimulated by
oxaliplatin adducts . -
pubmed
CONCLUSIONS : The synergistic cell killing is caused by a mechanistic interaction that requires the initiation of
XPF - dependent excision repair in response to
oxaliplatin adducts , and the inhibition of that process by
fludarabine incorporation into the repair patch . -
pubmed
Further reduction of preparative conditioning to
busulfan resulted in acceptance of donor skin implanted at 2 weeks after transplantation of naive and
FasL - coated BMC , whereas third - party grafts were acutely rejected . -
pubmed
MS has provided a better understanding of the absorption of
beta - carotene , the most potent
provitamin A carotenoid , through the use of stable isotopes of
beta - carotene . -
pubmed
Preparation of
( 68 ) Ga - Citrate and
( 68 ) Ga - transferrin is described , with very high yield and high radiochemical purity (
RCP ) , which is ideally suited for routine clinical studies . -
pubmed
One of the major limitations of
( 67 ) Ga - Citrate SPECT is the delayed
post injection waiting time of 48 hrs , in contrast to 60 min post injection waiting with
( 68 ) Ga - Citrate . -
pubmed
This may be due to short half - life of
( 68 ) Ga ( 68 min ) , as it would have decayed one half - life at 60 min
post - imaging time , compared to the SPECT agent (
( 67 ) Ga ) , which would require 76 hrs to undergo one half - life . -
pubmed
Similarly , uptake of
( 68 ) Ga by liver , cardiac blood pool activity is much lower than
( 67 ) Ga at 60 min
post injection period , may be attributed to the faster decay of
( 68 ) Ga than
( 67 ) Ga . -
pubmed
In summary , preliminary reports suggest
( 68 ) Ga - Citrate PET /
CT is useful in the diagnosis of suspected
bone infections with reliable sensitivity , specificity , positive predictive value , negative predictive value and overall accuracy . -
pubmed
Adenylate kinase isozyme 4 (
AK4 ) belongs to a family of
nucleotide monophosphate kinases involved in energy metabolism . -
pubmed
Degradation of
chlorophenols by a sequential combination of hydrodechlorination (
HDC ) and catalytic wet
peroxide oxidation ( CWPO ) using a new magnetic
Pd - Fe /
gamma - Al2O3 catalyst has been studied . -
pubmed
The sequential combination of
HDC and
CWPO allows overcoming some of the drawbacks of both treatments applied independently . -
pubmed
The
HDC step achieves the complete dechlorination of
chlorophenols , so that the subsequent
CWPO does not lead to the formation of highly toxic chlorinated by - products and reduces significantly the organic load of the effluent . -
pubmed
Therefore , the effective combination of
HDC and
CWPO requires that the
HDC step achieves the complete dechlorination of
chlorophenols but no further hydrogenation is needed . -
pubmed
The
Pd - Fe /
gamma - Al2O3 catalyst showed a high activity in both
HDC and subsequent
CWPO of
chlorophenols being easily separated and recovered from the reaction medium due to its ferromagnetic properties . -
pubmed
In spite of a moderate loss of activity , the complete dechlorination of
chlorophenol and a negligible ecotoxicity of the final effluents were maintained upon successive applications of
HDC +
CWPO in a four - cycles test . -
pubmed
Arbuscular mycorrhizal (
AM ) fungi are the most abundant plant symbiont and a major pathway of
carbon sequestration in soils . -
pubmed
Changes in the environmental conditions and variability of the mycorrhizosphere may alter the diurnal patterns of productivity of
AM fungi , thereby modifying soil
carbon sequestration , nutrient cycling and host plant success . -
pubmed
We carried out a morphologic study of the endothelium with the aid of light and transmission electron microscopy and an immunohistochemical assessment to identify
adenosine ,
inducible nitric oxide synthase (
iNOS ) , and
vascular endothelial growth factor (
VEGF ) in the vein wall . -
pubmed
CONCLUSION : Endothelial integrity and
adenosine ,
iNOS , and
VEGF immunoreactivities were better preserved when the no - touch technique was used for vein graft harvesting . -
pubmed
Progression of
hepatic fibrosis was evaluated by histopathology ,
hydroxyproline content , and DWI - MRI to determine the apparent diffusion coefficient (
ADC ) . -
pubmed
Direct biomarkers for
liver fibrosis , including
transforming growth factor - beta1 ,
tissue inhibitor of matrix metalloproteinase-1 (
TIMP-1 ) ,
hyaluronic acid ( HA ) ,
procollagen type III amino - terminal peptide (
PIIINP ) and
osteopontin (
OPN ) , were measured . -
pubmed
The indirect biomarkers
aspartate and alanine aminotransferases ,
albumin and
bilirubin were also tested . -
pubmed
Urological examination revealed a negative urine , bilateral large
ureterohydronephrosis on USG , and serum
creatinine 231 - 250 micromol / l.
CT displayed the urinary bladder completely herniated into the scrotum with distal parts of both ureters and small intestine , and bilateral large
ureterohydronephrosis . -
pubmed
The anti -
tumor effect of
shikonin on
osteosarcoma by inducing
RIP1 and
RIP3 dependent necroptosis . -
pubmed
The cell
death induced by
shikonin could be mostly rescued by specific necroptosis inhibitor
necrostatin-1 , but not by general
caspase inhibitor
Z - VAD - FMK . -
pubmed
The protein levels of
RIP1 and
RIP3 in primary
tumor tissues were increased by
shikonin . -
pubmed
CONCLUSIONS :
Shikonin had prompt but profound anti -
tumor effect on both primary and metastatic
osteosarcoma , probably by inducing
RIP1 and
RIP3 dependent necroptosis . -
pubmed
For seven inhibitors , we find that with correctly converged normal mode estimates of the configurational entropy , we can correctly distinguish inhibitors in agreement with experimental data for both the MMPBSA and MMGBSA methods and thus have the ability to rank the efficacy of binding of this selection of drugs to the
protease ( no account is made for free energy penalties associated with protein distortion leading to the over estimation of the binding strength of the two largest inhibitors
ritonavir and
atazanavir ) . -
pubmed
Saturation of
beta 2 - microglobulin was low ( 0.34 + - 0.1 ) and decreased discretely with time ( r ( 2 ) = 0.15 , P & lt ; 0.05 ) and significantly with
TMP increases ( r ( 2 ) = 0.31 , P & lt ; 0.01 ) . -
pubmed
Twenty microbial isolates that precipitated macroscopic
CaCO3(s ) when grown on
B4 media supplemented with
calcium acetate or
calcium citrate were identified . -
pubmed
Three
anhydrous CaCO3(s ) polymorphs (
calcite ,
aragonite , and
vaterite ) were identified by mu - XRD , and
calcite and
aragonite were also identified based on
TEM lattice - fringe d value measurements . -
pubmed
The obtained
lectin preparation ( abbreviated PIFA ) is a
glycoprotein with 6.5 + -1 %
carbohydrates , molecular mass of 64 kDa , consisting of four identical subunits . -
pubmed
Cellulose nanocrystals ( CNs ) were prepared from tunicate by enzymatic hydrolysis ( ECN ) ,
TEMPO - mediated oxidation ( TCN ) and acid hydrolysis (
ACN ) . -
pubmed
The final
PAN nanofibers were characterized by scanning electron microscopy ( SEM ) , energy dispersive spectrometer ( EDS ) , transmission electron microscopy (
TEM ) ,
TEM - selected area electron diffraction ( SAED ) , X - ray diffraction ( XRD ) and thermogravimetric analysis (
TGA ) . -
pubmed
SEM ,
TEM micrographs and SAED patterns confirmed homogeneous dispersion of the
silver nanoparticles which were composed of monocrystals with diameters 20 - 30 nm . -
pubmed
EDS and XRD results showed that these monocrystals tended to form
face - centered cubic single
silver . -
pubmed
Various analyses were performed on parameters including
body weight ,
tumor weight , in situ expression of
MMP-2 and
VEGF , and the plasma concentrations of
epinephrine ,
norepinephrine and
glucocorticoid . -
pubmed
Placental Barrier and
Autism Spectrum Disorders : The Role of
Prolactin and
Dopamine on the Developing Fetal Brain . -
pubmed
Dopamine and
prolactin exhibit opposite effects on lactation . -
pubmed
However , a possible role for increased
prolactin /
dopamine ratio in postpartum mood and
thought disorders and as a prognostic indicator of the mother 's future mental health has not been well investigated . -
pubmed
This review article explores a possible link between in - utero exposure to a high maternal
prolactin /
dopamine ratio and subsequent development of
autism spectrum disorders . -
pubmed
This approach was based on combining the two privileged structures ,
BS and
THQ , which are known to be active . -
pubmed
The number of
GPIIb / IIIa receptors occupied by the drugs was assayed by flow cytometry using two
CD61 surface
fluorescein antibodies . -
pubmed
Naringin showed significantly greater percentage occupation of
GPIIb / IIIa receptors than did
naringenin ( 14.82 + -0.81 % vs. 3.90 + -0.55 % ) , and
esculetin returned significantly higher values than
fraxetin and
coumarin ( 12.47 + -0.97 vs. 7.53 + -0.49 and 7.90 + -0.69 respectively ) . -
pubmed
Naringin was the best antiplatelet compound , showing the greatest antiplatelet activity and the highest percentage binding of
GPIIb / IIIa receptors . -
pubmed
Here , a potential application of the forward osmosis ( FO ) filtration process with
ammonium bicarbonate (
NH4HCO3 ) as a draw solution (
DS ) was investigated to remediate membrane bioreactor - treated LL ( M - LL ) . -
pubmed
In conclusion , this novel FO system using
NH4HCO3 as the
DS provides a cheap and efficient protocol to effectively remove
PAHs and other pollutants in LL , and the diluted
DS can be directly applied to crops as a liquid fertilizer , indicating that this technique is effective and eco - friendly for the treatment of different types of
LL . -
pubmed
Indeed ,
alpha - synuclein accumulation can impair synaptic
dopamine release and induces the
death of nigrostriatal neurons . -
pubmed
Considering the known effects of
TMS and the pathophysiology of
traumatic brain injury (
TBI ) ,
TMS was proposed to have potential for treating this condition . -
pubmed
The modified neurological severity score of the
TMS group was lower compared with the control group at
post -
TBI day 14 . -
pubmed
According to the results of
hematoxylin eosin staining , relative
cerebral parenchyma loss was lower at
post -
TBI day 28 in the
TMS group compared with the control group . -
pubmed
A significantly higher rate of neuron survival at day 28 ( P<0.05 ; indicated by
NeuN ) and a significantly reduced rate of apoptosis at days 7 and 14 ( P<0.05 ; indicated by
caspase-3 ) were observed in the perilesional zone of the
TMS group , as compared with the control group . -
pubmed
HES1 ,
KLF4 ,
STAT3 ,
IL-10 and
SOCS3 levels were determined using western blotting and qRT - PCR , while
phospho -
STAT3 levels were assessed by western blotting . -
pubmed
Field surveys of the impact of environmental
estrogen ( EE ) pollution in aquatic wildlife have been conducted using
vitellogenin (
VTG ) as a biomarker to evaluate the influence of EE . -
pubmed
Mean plasma
VTG and
E2 levels exhibited similar changes , increasing in the yolk vesicle stage and peaking in the tertiary yolk stage in females . -
pubmed
However , plasma
VTG and
E2 levels showed no significant changes in males , remaining at low levels throughout the reproductive cycle . -
pubmed
6 - Substituted Hexamethylene Amiloride (
HMA ) Derivatives as Potent and Selective Inhibitors of the
Human Urokinase Plasminogen Activator for Use in
Cancer . -
pubmed
This study reports the discovery of
6 - substituted HMA analogs that show nanomolar potency against
uPA , high selectivity over related
trypsin - like serine proteases , and minimal inhibitory effects against
epithelial sodium channels (
ENaC ) , the diuretic and antikaliuretic target of
amiloride . -
pubmed
The results support further evaluation of
6 - substituted HMA derivatives as
uPA - targeting anticancer drugs . -
pubmed
UHPLC /
MS /
MS was used for the analysis of
IVM faecal concentration . -
pubmed
High
CHOL content is found to exert strong inhibitory effects on
Kv channels . -
pubmed
Studying
anemia of chronic disease and
iron deficiency in patients with
rheumatoid arthritis by
iron status and circulating
hepcidin . -
pubmed
Functional iron deficiency in
ACD can be attributed to overexpression of the main
iron regulatory hormone
hepcidin leading to diversion of
iron from the circulation into storage sites resulting in
iron - restricted erythropoiesis . -
pubmed
Complete blood counts , serum
iron , total
iron binding capacity ,
ferritin , and
hepcidin-25 were assessed . -
pubmed
It was strongly and positively correlated with
ferritin ( P<0.001 ) , while
hemoglobin , serum
iron , and total
iron binding capacity were negatively correlated with
hepcidin ( P=0.016 , 0.022 and & lt;0.001 , respectively ) . -
pubmed
ATP hydrolysis activity catalyzed by
chloroplast and proteobacterial
ATP synthase is inhibited by their epsilon subunits . -
pubmed
Moreover , systematic
post - characterizations further reveal that the optimal
Ni -
FeS2 - 0.5 sample completely converts to amorphous
Ni - doped FeOOH via an in situ electrochemical transformation with yolk - shell structure well - preserved under the OER conditions . -
pubmed
Mechanisms of Blockade of the
Muscle - Type Nicotinic Receptor by
Benzocaine , a Permanently Uncharged Local Anesthetic . -
pubmed
Many of them are protonated at physiological pH , but
benzocaine (
Bzc ) is permanently uncharged , which is relevant because the effects of LAs on
nicotinic acetylcholine ( ACh ) receptors (
nAChRs ) depend on their presence as uncharged or protonated species . -
pubmed
Co - application of
ACh with increasing concentrations of
Bzc showed that
Bzc reversibly blocked
nAChRs . -
pubmed
Besides ,
ACh and
Bzc co - application enhanced
nAChR desensitization . -
pubmed
When
Bzc was just pre - applied it also inhibited IACh , by blocking closed ( resting )
nAChRs . -
pubmed
The electrophysiological results indicate that Bzc effects on
nAChRs are similar to those of
2,6 - dimethylaniline , an analogue of the hydrophobic moiety of
lidocaine . -
pubmed
Furthermore , docking assays on models of the
nAChR revealed that
Bzc and
DMA binding sites on
nAChRs overlap fairly well . -
pubmed
These results demonstrate that
Bzc inhibits
nAChRs by multiple mechanisms and contribute to better understanding both the modulation of
nAChRs and how LAs elicit some of their clinical side effects . -
pubmed
In this work , three chemically distinct treatments used to form structurally and mechanically stable scaffolds - EDC -
NHS ,
genipin and
tissue transglutaminase - are investigated for their effect on
collagen piezolectricity . -
pubmed
Crosslinking with
EDC - NHS is noted to produce a distinct self - assembly of the fibres into bundles roughly 300 nm in width regardless of the
collagen origin . -
pubmed
Effects of
palm oil consumption on biomarkers of
glucose metabolism : A systematic review . -
pubmed
Dietary
fatty acids has also been linked to affect
glucose metabolism and
insulin sensitivity . -
pubmed
This systematic review is aimed at critically evaluating the available evidence on the association of
palm oil with the biomarkers of
glucose metabolism as compared to other vegetable oils . -
pubmed
Insignificant effects were also seen on fasting
insulin when compared to partially hydrogenated
soybean oil [ 1.72pmol / L ( -11.39,14.84 ) P = 0.80 , I2 = 12 % ] and
olive oil diet [ -0.14pmol / L ( -4.87,4.59 ) P = 0.95 , I2 = 0 % ] . -
pubmed
CONCLUSION : Current evidence on the effects of
palm oil consumption on biomarkers of
glucose metabolism is poor and limited to only healthy participants . -
pubmed
We conclude that little or no additional benefit will be obtained by replacing
palm oil with other oils rich in
mono or polyunsaturated fatty acids for changes in
glucose metabolism . -
pubmed
Tagging of
TurboID , an improved promiscuous
biotin ligase that biotinylates neighboring proteins , to the C terminus of
caspases revealed the differences among the neighbors of executioner caspases . -
pubmed
Surprisingly , energy - related nutrients such as
amino acids / peptides and
tricarboxylic acid cycle - related metabolites were found at higher levels in
SI pollinations than in SC pollinations . -
pubmed
PURPOSE OF REVIEW : This article aims to give a state - of - the - art assessment of treatment options for
bloodstream infection because of
ceftriaxone - resistant Gram - negative bacilli , especially those caused by extended - spectrum
beta - lactamase (
ESBL ) or AmpC - producing Enterobacteriaceae . -
pubmed
Therefore , the mechanisms for induction of MtDNAcn and
MN by
benzene may be similar from exposure to low doses but different from high doses . -
pubmed
Similar increase of
MN frequencies and MtDNAcn may be due to oxidative stress induced by
benzene at low concentrations , while higher concentrations may start to initiate the cell
death pathway . -
pubmed
Meanwhile ,
TSA substantiated adequate power to demonstrate the relationship between
SOX2 -
OT expression and OS . -
pubmed
MgSO4 intervention in women at imminent risk for
preterm birth decreased the offspring 's
CP risk ( meta - analysis RR 0.68 , 95 % CI 0.54 - 0.85 ;
TSA RR 0.69 , 95 % CI 0.48 - 0.97 ) . -
pubmed
In addition , increasing in vivo studies further indicated that
migraine - related changes such as
CGRP increase ,
inflammation ,
nitric oxide increase , and spontaneous behavior problems can be reduced by UR extraction and its active constituents . -
pubmed
Biohybrid colloids were fabricated based on electrostatic complexation between anionic
TEMPO - oxidized cellulose nanofibrils ( TO -
CNF ) and cationic hen egg white
lysozyme (
HEWL ) . -
pubmed
Using an Ordinal Approach to Compare Outcomes Between
Vancomycin Versus
Ceftaroline or
Daptomycin in
MRSA Bloodstream Infection . -
pubmed
Tetrahydroisoquinolines derived from
catecholamines or
DOPA : effects on
brain tyrosine hydroxylase activity . -
pubmed
The
enzyme molecule is a tetramer of the alpha 2
beta 2 type . -
pubmed
Transketolase contains about two moles of
TPP per mole of protein and does not require metal ions for its catalytic activity . -
pubmed
An 11 - year - old Jewish girl of Turkish extraction with
abetalipoproteinaemia was found to be homozygous for
haemoglobin Szuhu ( beta80 Asn leads to
Lys ) . -
pubmed
[ Successful use of the
erbium - Yag laser in an
IVF program ] . -
pubmed
mansoni antigens in particular are implicated in the immune - dependent action of
PZQ : a 200 kDa glycoprotein and a 27 kDa antigen with non - specific
esterase activity . -
pubmed
A kit formulation for preparation of
iodine-123 - IBZM : a new
CNS D-2 dopamine receptor imaging agent . -
pubmed
The nature of these replacements in this
HLA - B35 variant is likely to affect
peptide binding and recognition by T lymphocytes . -
pubmed
There were significant reductions ( p less than 0.03 ) in percent recovery ( 37 degrees C and 24 degrees C ) of cardiac output ,
stroke work , and
adenosine triphosphate level in the groups that contained 0 or 15 mmol / L
Mg2 + as the
calcium concentration was increased from 0.05 to 4.5 mmol / L.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
Effect of amygdala kindling on
GABA neurons : histochemical and biochemical detection of
GABA - T activity . -
pubmed
The yield of purified
enzyme was 41 % and the specific activity was 592
o - nitrophenyl beta - D - galactopyranoside U / mg at 37 degrees C. Two
isozymes were present , but only one subunit was detected , having a mol . -
pubmed
At the
electron microscopic level ,
PRL - containing cells were visualized using the immunogold procedure . -
pubmed
The two binding curves were not completely parallel , suggesting the existence of slight variations of the
amino acid sequences of
PRL in the two species . -
pubmed
1000 - fold by sequential
( NH4 ) 2SO4 fractionation , gel filtration , ion - exchange and affinity chromatography on
calmodulin - Sepharose 4B. -
pubmed
The purified
protein kinase is completely dependent on
Ca2 + and
calmodulin , and phosphorylates a 20 kDa light - chain subunit of intact
gizzard myosin , suggesting that it belongs to a class of enzymes known as
myosin light - chain kinase (
MLCK ) . -
pubmed
The apparent Km values of the putative pancreatic
MLCK for
ATP ( 73 microM ) ,
gizzard myosin light chains ( 18 microM ) and
calmodulin ( 2 nM ) are similar to those reported for
MLCKs isolated from smooth muscle , platelet and other sources . -
pubmed
After separation from unbound ligand , cell - bound
IFN was extracted with the detergent
digitonin yielding soluble and insoluble complexes of
IFN and receptor , together with a certain amount of uncomplexed
IFN . -
pubmed
The total ( soluble and insoluble ) of complexed
IFN obtained after
digitonin extraction was a constant fraction ( 0.62 ) of the total cell - bound radioactivity , being independent of the concentration of
IFN added to the cells ( less than pM to greater than nM ) , and of the time of incubation ( 1 min to 20 h ) . -
pubmed
Ambulatory activity ( counts ) in the control periods was 298.2 + /- 20.1 in the dark , 35.6 + /- 9.4 in the light , and 343.9 + /- 24.1 over 24 h. After administration of
STZ ( 60 mg / kg , ip ) , the ambulatory activity gradually decreased [ at 3 days : 165.0 + /- 12.3 in the dark ( P less than 0.001 ) , 34.0 + /- 10.5 in the light ( P =
NS ) , and 199.0 + /- 10.5 over 24 h ( P less than 0.001 ) . -
pubmed
To prevent late
thrombosis of saphenous vein aortocoronary grafts , it appears reasonable that decreasing the ratios of total
cholesterol /
HDL cholesterol and of
LDL cholesterol /
HDL cholesterol , refraining from smoking , and controlling other risk factors for
atherosclerosis should be advised . -
pubmed
We have conducted phase I and II trials of topical
tretinoin (
retinoic acid and
Retin - A ) delivered by means of a cervical cap and inert
collagen sponge system . -
pubmed
The activity of the tagged gene as well as of the resident gene was significantly inhibited by treatment of the transgenic plants with the herbicide
norfluorazon , indicating that this gene activity is dependent on the presence of functional
chloroplasts in the leaves . -
pubmed
Characterization of
septic shock serum
PLA2 revealed a
calcium - dependent enzyme with absolute
2 - acyl specificity with a pH optimum of 7.5 . -
pubmed
Chloroquine ,
chlorpromazine ,
dexamethasone base ,
dexamethasone sodium phosphate ,
indomethacin ,
lidocaine ,
oleic acid ,
palmitic acid ,
promethazine ,
trans - retinoic acid ,
rutin and
dl - alpha - tocopherol were all studied over the range of 10(-2 ) to 10(-7 ) M. All agents , with the sole exception of
dexamethasone base , inhibited
PLA2 activity at concentrations greater than 10(-3 ) M.
PLA2 inhibition by
dexamethasone sodium phosphate was factitious , due to the formation of
calcium - phosphate complexes . -
pubmed
Since serum
PLA2 levels correlate with mortality in both experimental
endotoxemia and clinical gram - negative
septic shock , and
chlorpromazine was previously shown to improve survival in these conditions , we postulate that its therapeutic efficacy resides at least in part in its
PLA2 - inhibitory activity . -
pubmed
The
HSS reference patient improved after
oxygen therapy , diuretics , antibiotics , and relief of
OSA . -
pubmed
Damage effect of chronic intoxication by
CCl 4 on structural organization of liver microsomes and
cytochromes ( b ) 5 and
P-450 . -
pubmed
The purified
enzyme catalyzes the oxidation of
methanol and
formaldehyde to
formate ; other
primary alcohols are oxidized to their corresponding
aldehydes . -
pubmed
Ammonium ions are required for
enzyme activity . -
pubmed
4 The ineffectiveness of
atenolol compared to the other
beta - adrenoreceptor blocking drugs may be related to the finding that , in acute experiments ,
atenolol inhibited tissue uptake of
3H - NA . -
pubmed
Control of
opiate receptor -
adenylate cyclase interactions by
calcium ions and
guanosine-5 '- triphosphate . -
pubmed
Activation of either
opiate receptors by 10 microM
morphine or
alpha - adrenergic receptors by 10 microM
norepinephrine inhibits
adenylate cyclase by 55 % in the absence of
Ca2 + ions , and inhibits the
Ca2 + -dependent activation of
adenylate cyclase by more than 90 % . -
pubmed
Concentrations of
Ca2 + ions greater than 0.1 mM inhibit
adenylate cyclase and also reduce the extent inhibition of
adenylate cyclase by
morphine but not by
norepinephrine . -
pubmed
Guanosine-5 '- triphosphate ( 0.1 - 1 microM ) is required for inhibition of
adenylate cyclase by
morphine . -
pubmed
The results show that
morphine inhibits
adenylate cyclase by a
guanosine-5 - triphosphate - dependent process and that the extent of inhibition of
adenylate cyclase by
morphine or
norepinephrine is a function of the
Ca2 + ion concentration and the proportion of
adenylate cyclase molecules that are activated or inhibited by
Ca2 + ions . -
pubmed
It was almost 50 % higher than
phosphatidylcholine in the unsaturated fat group and coincided with the accumulation of an
apolipoprotein E - rich high density lipoprotein . -
pubmed
In a fatal case of acute
parathion ingestion ,
paranitrophenyl sulphate , the
paranitrophenyl glucuronide and free
paranitrophenol excretion on day 3 were quantitated using a HPLC reverse method with a
C18 column following sample preparation using a
C18 mini - column and
methanol elution . -
pubmed
Effects of
tibolone on
lipoprotein ( a ) and
HDL subfractions . -
pubmed
OBJECTIVE : To determine the effects of
tibolone , a synthetic
steroid used to alleviate
climacteric symptoms and prevent
osteoporosis , on lipoprotein metabolism , with particular reference to
lipoprotein ( a ) levels and
HDL subfraction profiles . -
pubmed
There were significant decreases in
triglycerides and
VLDL cholesterol and no significant change in
LDL cholesterol . -
pubmed
There was a significant reduction of 18 % in
HDL cholesterol and a 26 % reduction in the
HDL2 -
HDL3 ratio . -
pubmed
CONCLUSION : The reduction in
lipoprotein ( a ) levels may have a beneficial effect on cardiovascular risk , which could go some way towards balancing the potentially adverse effect on the cardiovascular system caused by the reduction in HDL
cholesterol . -
pubmed
Vasotocin treatment increased
adenylate cyclase activity in glomeruli and in collecting ducts and did not modify it in proximal convoluted tubules and in early and late distal tubules . -
pubmed
In glomeruli , the hormonal stimulation resulted mainly in a decrease in the Km value for
adenylate cyclase , which means a higher affinity for substrate (
ATP ) to the enzyme , whereas the response to
forskolin was accounted for by increases both in affinity for substrate and in maximal
adenylate cyclase velocity . -
pubmed
This receptor apparently is coupled to a G - protein which activates
cGMP - phosphodiesterase to modulate
cGMP levels and thus a
cGMP - gated cation channel . -
pubmed
Thus , across a wide range of species ,
G ( o ) is present in retinal bipolar cells , and at least some of these are depolarizing and
APB - sensitive . -
pubmed
Changes in plasma
pentosidine levels after transplantation were compared to changes in simultaneously obtained
glycohemoglobin levels . -
pubmed
Kidney - pancreas transplantation resulted in normal
glycohemoglobin levels within three months but offered no advantage over kidney transplantation alone in the partial correction of plasma
pentosidine levels . -
pubmed
The unifactorial analysis ( UFA ) suggested that the following factors were associated to an statistically significant increase in the mortality due to
sepsis : age above 70 ; male sex ; presence of
shock upon hospitalization ; nosocomial etiology ; high levels of
urea ,
creatinine and
LDH ;
proteinuria ; family of the causal germ ( gram - positive coccus ) and underlying pathology . -
pubmed
The relative risks ( RR ) or " odd ratios " of
creatinine and
LDH were , respectively , 2.8 and 2.9 in the UFA ; 3 and 3.3 in the multivariant analysis ( MVA ) . -
pubmed
The results of the univariant and multivariant analysis showing the relative risk ( odd ratio ) associated to each of these variables with statistical significance , demonstrate that the mortality rate due to
sepsis increases with age , nosocomial etiology and elevation of
creatinine and
lactodehydrogenase (
LDH ) levels . -
pubmed
Urapidil , an antihypertensive agent with dual action (
alpha 1 - adrenergic antagonist and
5HT1A agonist ) is well established in the treatment of
arterial hypertension . -
pubmed
These two tissues were representative of both isoforms , SI- or
SI+ , and displayed the same [
3H]InsP3 - binding characteristics . -
pubmed
Deletion of the peptide 316 - 352 containing the
SI segment greatly reduced
InsP3 binding [ Miyawaki , Furuichi , Ryou , Yoshikawa , Nakagawa , Saitoh and Mikoshiba ( 1991 ) Proc . -
pubmed
The antibodies against the
SI region or against residues 337 - 349 did not modify the binding of
[ 3H ] InsP3 in the cortical membranes rich in the SI+ isoform or in cerebellar membranes . -
pubmed
We suggest that the domain with the
SI region may be involved in a function other than
InsP3 - induced
Ca2 + release . -
pubmed
The purpose of the exhibition was to inform members of the progress on patient education , guidance of diet and exercise treatment , the compliance situation on self - monitoring of blood
glucose , self - injection of
insulin , etc . -
pubmed
Using colorimetric assays , we were able to show
aryl sulphatase B activity ( 18.5 + /- 3.7 nM
nitrocatechol/10(6 ) cells ) and detected
peroxidase ( 1.86 + /- 0.03 ng/10(6 ) cells ) in EGC homogenates . -
pubmed
Bilateral testicular function was determined 60 days after experimental
torsion by measuring testis weights , daily sperm production (
DSP ) , and testicular venous
testosterone concentrations . -
pubmed
RESULTS :
SOD +
catalase and
SOD +
catalase+
verapamil treatments caused significant rescue of tests function following 1 hr . -
pubmed
Effects of
N - methyl - D - aspartate - receptor blockade on high -
potassium - induced
neuronal death and
glutamate release . -
pubmed
In this study we determined the effects of the competitive
N - methyl - D - aspartate ( NMDA )- receptor antagonist
CGP 40116 . -
pubmed
The aim of the present investigation was to examine if new cementum (
NC ) formed during
cyclosporin A (
CsA ) administration was maintained after suspension of the treatment . -
pubmed
These results suggest that ( a )
NC deposition and its functional relations with the adjacent connective tissue are not reversible after cessation of
CsA treatment and ( b ) in the presence of cervical
NC spurs the amount of connective tissue attachment on the root surfaces is increased . -
pubmed
To block
P - gp function ,
cyclosporin A was administered . -
pubmed
This indicates complete inhibition of
P - gp mediated
[ 11C ] verapamil efflux . -
pubmed
We conclude that
cyclosporin A can fully block the
P - gp function in the blood brain barrier and the testes and that PET enables the in vivo measurement of
P - gp function and reversal of its function non - invasively . -
pubmed
IL-13 dose - dependently ( 1 - 100 pmol / L ) reduced the incorporation rate of
[ 3H ] thymidine in hOB cells by more than 50 % . -
pubmed
Blood concentrations of
vasopressin ,
neuropeptide FF and
prolactin are increased by high - dose right unilateral
ECT . -
pubmed
The maximal elevations in circulating
vasopressin and
prolactin concentrations were 680 % and 950 % , respectively . -
pubmed
The
neuropeptide FF concentration returned to baseline level at 10 min and the
vasopressin concentration at 25 min after
ECT . -
pubmed
In fact , these three novel metal -
thiosemicarbazone compounds kill
Pam 212 - ras cells through apoptosis induction . -
pubmed
Crystallographic analysis of
triclosan bound to
enoyl reductase . -
pubmed
mazei
Go1 comprises 12 genes that are referred to as
fpoA , B , C , D , H , I , J , K , L , M , N , and O. Analysis of the deduced
amino acid sequences revealed that the enzyme is closely related to proton translocating
NADH dehydrogenases of respiratory chains from bacteria (
NDH-1 ) and eukarya ( complex I ) . -
pubmed
Like the
NADH - dependent enzymes , the
F ( 420 ) H ( 2 ) dehydrogenase is composed of three subcomplexes . -
pubmed
Characterization of
SB-269970 - A , a selective
5 - HT ( 7 ) receptor antagonist . -
pubmed
and the non - selective
5 - HT ( 7 ) receptor antagonist
metergoline ( 0.3 - 3 mg kg(-1 ) s.c . ) -
pubmed
, suggesting a role for
5 - HT ( 7 ) receptor stimulation in
5 - CT induced
hypothermia in guinea - pigs . -
pubmed
Liver histopathology ,
hydroxyproline content ,
prolyl hydroxylase activity ,
collagen I and III mRNA expression and the evolution of transformed Ito cells into myofibroblasts were assessed . -
pubmed
2 - Phosphonomethyl pentanedioic acid (
2 - PMPA ) is a potent and selective inhibitor of
glutamate carboxypeptidase II (
NAALADase ) , and has shown robust neuroprotective activity in both in vitro and in vivo models of
ischemia . -
pubmed
In the brain ,
glutamate carboxypeptidase II (
GCPII ) ( EC3.4.17.21 ) hydrolyzes the neuropeptide
N - acetylaspartylglutamate (
NAAG ) to
glutamate and
N - acetylaspartate . -
pubmed
Binding exhibited similar pharmacological properties to
GCPII enzyme activity , including
chloride dependency ,
cobalt stimulation and inhibition by
phosphate and
quisqualate . -
pubmed
The binding of
[ ( 3 ) H ] 2 - PMPA also showed tissue specificity in that tissues previously reported to be devoid of
GCPII enzymatic activity were devoid of
[ ( 3 ) H ] 2 - PMPA binding . -
pubmed
[ ( 3 ) H ] 2 - PMPA binding represents an additional probe for the study of
GCPII activity , and may be useful as a high throughput screening assay . -
pubmed
BACKGROUND : Selective deamidation of
glutamine residues by
tissue transglutaminase (
tTG ) turns gliadin peptides into stronger activators of T cells from
celiac disease (
CD ) patients . -
pubmed
The reviewed literature includes the effects of changing the conditions , intermediates and kinetic traps , mutations , similar topologies , fragment complementation experiments , fragments and pathways , focusing on one specific well - studied example , that of the
dihydrofolate reductase ,
chaperones , and
chaperonines , in vivo vs. in vitro folding , still using the
dihydrofolate example , amyloid formation , and molecular " disorder " . -
pubmed
A phase I -
II study of
docetaxel -
ifosfamide -
cisplatin (
DIP ) combination chemotherapy regimen in advanced
nonsmall cell lung cancer . -
pubmed
G - CSF (
lenograstin ) was administered from days 4 - 13 . -
pubmed
The
dose - limiting toxicity (
DLT ) was reached at
DL4 (
Docetaxel : 100 mg / m2 -
Ifosfamide : 5 g / m2 -
Cisplatin : 100 mg / m2 ) consisting of 2 cases of
febrile neutropenia (
FN ) , and
DL3 (
Docetaxel : 100 mg / m2 -
Ifosfamide : 5 g / m2 -
Cisplatin : 80 mg / m2 ) was considered as the maximum tolerated dose ( MTD ) and recommended for further phase II testing . -
pubmed
In the present phase I -
II study ,
DIP appears highly active and tolerable in advanced
NSCLC in the outpatient setting . -
pubmed
The
telomerase - transduced cells recovered a chondrocyte - specific gene expression pattern following culture on
polyHEMA - coated dishes . -
pubmed
The present study examined a possible molecular mechanism involved in
cAMP elevators - induced inhibition of
IL-12 p40 expression in response to LPS . -
pubmed
Subsequent studies revealed that
cAMP - elevators blocked phosphorylation of
p38 MAPK , but did not affect the activity of
NF - kappaB binding to IL-12 promoter ( -136/-112 ) . -
pubmed
This is the first report that
cAMP elevators inhibit LPS - induced
IL-12 production by a mechanism that is associated , at least in part , with
p38 - dependent inhibition by
cAMP signaling pathways . -
pubmed
The higher the
vWF levels or the lower the
NO levels , the more severe in
PIH . -
pubmed
There was a negative correlation between the plasma
vWF and
NO levels . -
pubmed
CONCLUSION : The results suggest that plasma
vWF and
NO concentration can be used as indicators in the judgement of the severity of
PIH . -
pubmed
Carbohydrate structural units in
glycoproteins and polysaccharides as important ligands for
Gal and
GalNAc reactive
lectins . -
pubmed
Glycoproteins (
gps ) contain many
carbohydrate epitopes or
crypto - glycotopes for
Gal and
GalNAc reactive
lectins . -
pubmed
They are : F ,
GalNAcalpha1 - -& gt;
3GalNAc ; A ,
GalNAcalpha1 - -& gt;3Gal ; T ,
Galbeta1 - -& gt;
3GalNAc ; I ,
Galbeta1 - -& gt;
3GlcNAc ;
II ,
Galbeta1 - -& gt ;
4GlcNAc ; B , Galalpha1 - ->3Gal ; E , Galalpha1 - ->4Gal ; L , Galbeta1 - -> ;
4Glc ; P ,
GalNAcbeta1 - -& gt;3Gal ; S ,
GalNAcbeta1 - -& gt;4Gal and
TN ,
GalNAcalpha1 - -& gt;
Ser(
Thr ) . -
pubmed
TN , which is an important marker for breast /
colon cancer and vaccine development , exists only in
O - glycans . -
pubmed
To facilitate the selection of
lectins that could serve as structural probes , the
carbohydrate binding properties of
Gal /
GalNAc reactive
lectins have been classified according to their highest affinity for structural units and their binding profiles are expressed in decreasing order of reactivity . -
pubmed
Hence , the binding relationship between
glycoproteins and
Gal /
GalNAc specific
lectins can be explored . -
pubmed
Endothelial and subendothelial cells expressed none to scant , very weak
progesterone and
estrogen receptor positivity . -
pubmed
Thus , increased expression of
hCTR1 had a substantially larger effect on the cellular pharmacology of
copper than
cisplatin . -
pubmed
Thus , although increased expression of
hCTR1 mediates greater cellular accumulation of
copper and
cisplatin ,
hCTR1 delivers these compounds into intracellular compartments from which they do not have ready access to their key cytotoxic targets . -
pubmed
Facial axotomy led to a gradual increase of
B7.2 immunoreactivity ( IR ) on microglial cell surface ; similar effects were also observed following application of
LPS , but both effects were not additive , suggesting overlapping or saturated signaling pathways . -
pubmed
The adsorption of
fibrinogen (
Fb ) and
bovine serum albumin onto polycrystalline
Au coated with
HS ( CH2 ) 3O ( CH2CH2O ) 5CH3 was determined by surface plasmon resonance from bare
Au ( 0 % coverage ) to the complete ( approximately 100 % coverage ) self - assembled monolayer ( SAM ) . -
pubmed
On the basis of our data , we propose a mechanism of
protein rejection by
oligo ( ethylene oxide ) (
OEO)-modified surfaces in terms of changes in free energy ( DeltaGsystem ; system = protein + surface ) due to oligomer conformational constriction over an area greater than the contact area . -
pubmed
Differential DNA recognition and cleavage by
EcoRI dependent on the dynamic equilibrium between the two forms of the
malondialdehyde -
deoxyguanosine adduct . -
pubmed
Hypoglycaemia induced by
insulin injection is a powerful stimulus to the hypothalamic - pituitary - adrenal (
HPA ) axis and drives the secretion of
corticotropin - releasing hormone and
vasopressin from the neurones in the paraventricular nucleus ( PVN ) , as well as the downstream hormones ,
adrenocorticotropic hormone and
corticosterone . -
pubmed
Regulation of glutamatergic mechanisms could be involved in the control of the
HPA axis during hypoglycaemic stress and one potential site of regulation might be at the receptors for
glutamate , which are expressed in the PVN . -
pubmed
Injection of
insulin induced a rapid fall in plasma
glucose concentrations , which was mirrored by an increase in plasma
corticosterone concentrations . -
pubmed
Moreover , the synergistic interaction of
gemcitabine and
paclitaxel was correlated to a block of the cells in the G0 /
G1 compartment of cell cycle and to an increase of apoptotic cells compared to each drug . -
pubmed
Glyceroneogenesis is a
phosphoenolpyruvate carboxykinase (
PEPCK)-dependent process by which
glycerol can be produced from precursors like
glutamine and therefore represents a truncated gluconeogenic pathway . -
pubmed
However , the demonstration that
PEPCK is massively expressed in these larval forms as compared to adult parasites , together with the observation that
3 - mercaptopicolinate , a specific inhibitor of
PEPCK , significantly reduces the effect of
glutamine on sporocyst growth , suggest that
glutamine could also be used for
glucose or
glycerol production . -
pubmed
Results of
[ 14C ] glutamine incorporation confirmed that neosynthesis of
glucose and mainly of
glycerol occurred in sporocysts and was dependent on
PEPCK activity . -
pubmed
Apart from light and
melatonin little is known about other systems from the periphery that may provide information to the
SCN . -
pubmed
BACKGROUND : The oral dual
endothelin ( ET ) antagonist
bosentan has been established as a cornerstone in the treatment of
pulmonary arterial hypertension (
PAH ) . -
pubmed
Considering the importance of
cAMP and
cGMP to disparate physiological functions including visual response , smooth muscle relaxation ,
platelet aggregation , immune response , and cardiac contractibility , perhaps the 200 or more splice isoforms of
PDE genes also play a major functional role . -
pubmed
In addition , we used a
PPAR - gamma antagonist ,
T0070907 , to determine the role of
PPAR - gamma during
ischemia . -
pubmed
Both DNA binding and
lipoprotein lipase expression were increased by the addition of the
PPAR - gamma agonist
rosiglitazone . -
pubmed
Finally , we found that
rosiglitazone - mediated protection after
stroke was reversed by the
PPAR - gamma antagonist
T0070907 . -
pubmed
Interestingly ,
infarction size was also increased by
T0070907 in the absence of
PPAR - gamma agonist , suggesting that endogenous
PPAR - gamma ligands may mitigate the effects of
cerebral ischemia . -
pubmed
3 - Beta - hydroxysteroid dehydrogenase1 (
HSD3B1 ) and
uridine diphosphate - glucuronosyltransferase 2B17 (
UGT2B17 ) are enzymes that inactivate
dihydrotestosterone in the prostate and may affect
dihydrotestosterone concentration in prostatic tissue . -
pubmed
The activities of these
enzymes were repressed by excess
phosphate ( 4 mM ) in the culture medium , but this repression was reversed upon the onset of
phosphate starvation in low
phosphate defined medium ( LPDM ) containing 0.2 mM of
phosphate at approximately the end of the exponential growth phase . -
pubmed
The expressions of the two
enzymes were also influenced by different
carbon sources , including
glucose ,
fructose ,
maltose ,
glycerol , and
sodium acetate at different levels . -
pubmed
The
APase activity was derepressed most profoundly in LPDM containing
fructose as a sole
carbon source . -
pubmed
However , the repression / derepression of the
enzyme by
phosphate was not observed in media containing
glycerol or
sodium acetate . -
pubmed
The highest levels of
PLase activity were detected in
LPDMsodium acetate , followed by
LPDM - fructose . -
pubmed
PLase was not fully repressed by high
phosphate concentrations when
sodium acetate was utilized as the sole
carbon source . -
pubmed
Cytosensing and evaluation of
cell surface glycoprotein based on a biocompatible
poly ( diallydimethylammonium ) doped
poly ( dimethylsiloxane ) film . -
pubmed
To escape from attack of lymphocytes , villous trophoblasts do not express classical
major histocompatibility complex ( MHC ) class I molecules . -
pubmed
A lipidomics approach revealed that
At4g24160 has additional
triacylglycerol lipase and
phosphatidylcholine hydrolyzing enzymatic activities . -
pubmed
Gene
alyPI was composed of a 1575 bp open reading frame encoding a protein of 57.4 kDa containing 524
amino acid residues with a signal peptide of 23
amino acids . -
pubmed
AlyPI was most active at 40 degrees C and pH 7.0 in the presencce of 0.1 mol / L
NaCl and stable over a broad range of pH , 6.0 - 10.6 . -
pubmed
Another nine
amino acid consensus region , YXRSELREM , only found in
G - specific alginate lyases previously existed in
AlyPI , which could degrade
sodium alginate , M blocks , and G blocks and appeared to be a broad substrate - specific
alginate lyase . -
pubmed
The effects of selective antagonists of the four
adenosine receptor subtypes were investigated on the airways responses of sensitized guinea - pigs to inhaled
ovalbumin to ascertain the role of
adenosine in these allergen responses , and compared with a
corticosteroid ,
dexamethasone . -
pubmed
Airway reactivity to inhaled
histamine ( 1 mM ) and inflammatory cell influx in bronchoalveolar lavage fluid were also determined 24 h after
ovalbumin challenge . -
pubmed
ZM241385 (
A ( 2A ) receptor antagonist ) did not affect these responses , whereas
DPCPX ( A ( 1 ) receptor antagonist ) exerted a small inhibition only of the
LAR . -
pubmed
MRS1706 (
A ( 2B ) receptor antagonist ) inhibited the
airways hyperreactivity and cellular influx and enhanced the EAR . -
pubmed
MRS1220 (
A ( 3 ) receptor antagonist ) inhibited the
airways hyperreactivity and cellular influx without affecting EAR and
LAR . -
pubmed
Dexamethasone inhibited the
ovalbumin - induced late
asthmatic responses ,
airways hyperreactivity and cellular influx . -
pubmed
The blockade of
airway hyperreactivity and inflammatory cell influx by
A ( 2B ) and A ( 3 ) receptor antagonists suggests that endogenous
adenosine is released by inhaled allergen and these responses are mediated via
A ( 2B ) and A ( 3 ) receptors in guinea - pigs . -
pubmed
The
adenosine released by allergen inhalation does not , however , appear to be involved in the EAR , but it may contribute to the
LAR via
A ( 1 ) receptors . -
pubmed
Here , we further characterize the mechanisms mediating this low frequency stimulation ( LFS)-induced LTP in area
CA1 of intact ,
urethane - anesthetized preparations . -
pubmed
Local application of the protein synthesis inhibitor
anisomycin in
CA1 blocked LFS - induced LTP , as did application of
H89 , an inhibitor of
protein kinase A. -
pubmed
A generalized model for correlation of the
CD spectra of
2 - cyclohexenones with their structures is presented . -
pubmed
Docosahexaenoic acid derivative prevents
inflammation and hyperreactivity in lung : implication of
PKC - Potentiated inhibitory protein for
heterotrimeric myosin light chain phosphatase of 17 kD in
asthma . -
pubmed
Moreover , treatment with
CRBM-0244 resulted in a decrease in
NF - kappaB activation and
cyclooxygenase-2 (
COX-2 ) overexpression triggered by
TNF - alpha . -
pubmed
The inhibition of
peroxisome proliferator - activated receptor - gamma with
GW9662 abolished the
CRBM-0244 - mediated anti - inflammatory effects . -
pubmed
CRBM-0244 reduced the
Ca ( 2 + ) sensitivity of bronchial smooth muscle through a decrease in the phosphorylation and expression of the
PKC - potentiated inhibitory protein for
heterotrimeric myosin light chain phosphatase of 17 kDa (
CPI-17 ) . -
pubmed
Furthermore , the presence of specialized enzymes such as
5 - lipoxygenase and
15 - lipoxygenase in the lung may convert
CRBM-0244 into active mediators , leading to the resolution of
inflammation . -
pubmed
After oral administration of
CRBM-0244 , airway leukocyte recruitment , airway mucus ,
ovalbumin - specific IgE , and proinflammatory markers such as
TNF - alpha and
COX-2 were markedly reduced . -
pubmed
Hence ,
CRBM-0244 treatment prevents
airway hyperresponsiveness ,
Ca ( 2 + ) hypersensitivity , and the overexpression of
CPI-17 in lung tissue . -
pubmed
This study characterized the
UGT enzyme selectivity of
niflumic acid (
NFA ) . -
pubmed
Higher
NFA concentrations ( 50 - 100 muM ) inhibited
UGT1A1 and
UGT2B15 but had little effect on the activities of
UGT1A3 ,
UGT1A4 ,
UGT1A6 ,
UGT2B4 ,
UGT2B7 , and
UGT2B17 . -
pubmed
Consistent with previous reports ,
APAP was glucuronidated by recombinant
UGT1A1 ,
UGT1A6 ,
UGT1A9 , and
UGT2B15 . -
pubmed
NFA concentrations in the range of 2.5 to 100 muM inhibited
APAP glucuronidation by
UGT1A1 ,
UGT1A9 , and
UGT2B15 but not by
UGT1A6 . -
pubmed
The mean V(max ) for
APAP glucuronidation by
HLM was reduced by 20 , 35 , and 40 % , respectively , in the presence of 2.5 , 50 , and 100 muM
NFA . -
pubmed
Taken together , the
NFA inhibition data suggest that
UGT1A6 is the major enzyme involved in
APAP glucuronidation . -
pubmed
By electrochemical junction with
iodide /
iodine based redox
electrolyte ,
perovskite QD - sensitized 3.6 mum - thick
TiO ( 2 ) film shows maximum external quantum efficiency ( EQE ) of 78.6 % at 530 nm and solar - to - electrical conversion efficiency of 6.54 % at
AM 1.5 G 1 sun intensity ( 100 mW cm(-2 ) ) , which is by far the highest efficiency among the reported inorganic quantum dot sensitizers . -
pubmed
Thereby , flow cytometry revealed high cellular association and internalization of anti -
IGF1 - R immunoliposomes ( soy
phosphatidylcholine (
SPC)/
cholesterol (
Chol)-
polyethyleneglycol (
PEG)-1H7 , 50.1 + -2.2 % ) . -
pubmed
Surface enhanced fluorescence (
SEF ) effect of
acridine orange fluorophore in the proximity of
silver nanoparticles ( NPs ) has been investigated experimentally in the aqueous solution system . -
pubmed
Compared to the normal population , the levels of
oxidized low - density lipoprotein (
LDL )
cholesterol are higher and the levels of
high density lipoprotein ( HDL )
cholesterol are lower . -
pubmed
Structure and dynamics of the two amphipathic
arginine - rich peptides RW9 and
RL9 in a lipid environment investigated by solid - state NMR and MD simulations . -
pubmed
However ,
amino acids present in the
carboxy - terminal region of
CD5 , were necessary for this effect , indicating that ITAM - independent sites were involved in the interaction of
c - Cbl with
CD5 . -
pubmed
Interactions between
oestrogen and the
renin angiotensin system - potential mechanisms for gender differences in
Alzheimer ' s disease . -
pubmed
Interactions between
oestrogen and the
renin angiotensin system ( RAS ) are reviewed and explored from the perspective where these interactions may modulate risk of developing
Alzheimer ' s disease (
AD ) . -
pubmed
Specifically ,
oestrogen prevents the production and action of
angiotensin II (
Ang II ) , thought to exert harmful effects of the RAS in both
hypertension and
AD , while also potentially facilitating
RAS - mediated
Abeta degradation . -
pubmed
Clinical trials targeting either the
RAS or
oestrogen systems for
AD prevention and treatment should perhaps give closer attention to key biochemical components of these pathways as potential confounders to primary and secondary outcome measures . -
pubmed
In a mesocosm experiment unfiltered seawater from the northern Baltic Sea was fertilized with inorganic nutrients and humic
carbon (
CNP ) , and only with inorganic nutrients (
NP ) . -
pubmed
The use of
atazanavir , compared with other
protease inhibitors , was associated with a lower likelihood of having a prolonged QTc . -
pubmed
The highest in vitro antioxidant activity determined by
DPPH radical scavenging was achieved with
rutin hydrolyzed by
hesperidinase . -
pubmed
There was no statistical difference between
xanthine oxidase inhibition by
rutin before and after hydrolysis . -
pubmed
pylori , in vitro , using a new photosensitising material (
PSM ) based on a
ruthenium ( II ) complex covalently bound to micrometric glass beads . -
pubmed
pylori isolates ( classified according to
cagA genotype , and
metronidazole -
clarithromycin resistance ) were used . -
pubmed
Subjects were classified as normoalbuminuric ( urinary
albumin - to -
creatine ratio [ UACR ] & lt;30 mg / g ) ,
microalbuminuric ( UACR & gt;=30 and & lt;300 mg / g ) , or
proteinuric ( UACR & gt;=300 mg / g ) . -
pubmed
They also presented greater measures of
body mass index and waist circumference , higher values of total and
LDL cholesterol , and lower values of HbA1c and serum
creatinine than subjects with
microalbuminuria or
proteinuria . -
pubmed
Molecular imaging and pharmacokinetics of
( 99 m ) Tc -
hTERT antisense
oligonucleotide as a potential
tumor imaging probe . -
pubmed
The uptake kinetics and imaging results of
( 99 m ) Tc -
hTERT antisense
oligonucleotides ( ASON ) in
hTERT - expressing cells were examined in vitro and in vivo . -
pubmed
The pharmacokinetics and
acute toxicity studies of
( 99 m ) Tc -
hTERT ASON were also performed . -
pubmed
Abstract Background :
Nephrotoxicity is a dose - limiting side effect of long - term use of
tenofovir , a
reverse transcriptase inhibitor that is used for the treatment of
human immunodeficiency virus ( HIV ) infection . -
pubmed
Interleukin-6 (
IL-6 ) and
thiobarbituric acid reactive substances ( T - BARS ) were used as markers of
inflammation and oxidative stress respectively . -
pubmed
Exposure of cells to
sphingosine 1 - phosphate induced pro - inflammatory responses characterized by
interleukin-6 ,
interleukin-8 , and
cyclooxygenase-2 up - regulations , as observed by ELISA and Western blot . -
pubmed
Strikingly , cell treatment with
sphingosine 1 - phosphate plus lipopolysaccharide resulted in the synergistic induction of
cyclooxygenase-2 , and
intercellular adhesion molecule 1 , as well as the secretion of
prostaglandin E2 , the soluble form of the
intercellular adhesion molecule 1 , and the pro - angiogenic factor
vascular endothelial growth factor - A. -
pubmed
As regards pro - osteogenic pathways ,
sphingosine 1 - phosphate induced
calcium deposition and the expression of the
calcification markers
bone morphogenetic protein-2 and
alkaline phosphatase , and enhanced the effect of lipopolysaccharide , an effect that was partially blocked by inhibition of
sphingosine 1 - phosphate receptors 3 / 2 signaling . -
pubmed
fumigatus
gliT , a
strain that is hypersensitive to
gliotoxin . -
pubmed
fumigatus
gliT when challenged with
gliotoxin . -
pubmed
fumigatus
gliT challenged with exogenous
gliotoxin . -
pubmed
Similarly ,
Rankl expression peaked in marrow cells within 3 days of
iron exposure ( 9.2 - fold ) . -
pubmed
By contrast ,
TPPPS1 exhibited other effects , and it induced the highest amount of
NO production , thereby indicating that
TPPPS1 had the best antioxidant activity . -
pubmed
Results indicated that TPPPS comprised three main components , among which ,
TPPPS1 mainly showed antioxidant effects , whereas
TPPPS2 and
TPPPS3 played key roles in immunomodulation , especially
TPPPS3 . -
pubmed
Moreover ,
BABA seed priming reduced
malondialdehyde content in the seedlings and enhanced the accumulation of
proline ,
total protein , total
carbohydrate ,
nitrate reductase activity , and activities of antioxidant enzymes like
guaiacol peroxidase and
superoxide dismutase . -
pubmed
Polymorphism of the
CYP3A5 gene and its effect on
tacrolimus blood level . -
pubmed
OBJECTIVES :
Tacrolimus is the cornerstone for immunosuppression in renal transplant and is metabolized by the
cytochrome P 450 3A (
CYP3A ) subfamily of enzymes in the liver and small intestine . -
pubmed
A polymorphism in intron 3 of the
CYP3A5 gene affects the expression of this enzyme and
tacrolimus trough blood levels . -
pubmed
Mean
tacrolimus level in the
CYP3A5 * 1 /* 1 group was 5.154 ng / mL ( range , 4.42 to 6.5 ng / mL ) ,
CYP3A5 * 1 /* 3 group was 5.348 ng / mL ( range , 3.1 to 9.87 ng / mL ) , and
CYP3A5 * 3 /* 3 group was 9.483 ng / mL ( range , 4.5 to14.1 ng / mL ) . -
pubmed
Tacrolimus drug level correlated well with presence or absence of
CYP3A5 polymorphisms . -
pubmed
Therefore ,
CYP3A5 polymorphism analysis before renal transplant may help determine the optimal dose of
tacrolimus in this population and prevent acute rejection episodes or
tacrolimus toxicity . -
pubmed
Herein , the synthesis of a supported
Ni -
Pt bimetallic nanocatalyst on mesoporous
ceria by a one -
pot evaporation - induced self - assembly method is reported . -
pubmed
Phase / structural analysis by XRD ,
TEM , and Auger electron spectroscopy was conducted to gain insight into the excellent catalytic performance of the
Ni -
Pt /
CeO2 catalyst . -
pubmed
Fludarabine (
FLU)-based conditioning was associated with decreased graft failure ( odds ratio 0 21 , P = 0 01 ) , decreased early mortality ( hazard ratio 0 25 , P = 0 01 ) and improved 5 - year overall survival ( FLU 87 8 % [ standard error (
SE ) 5 1 % ] versus non -
FLU 59 3 % [
SE 9 5 % ] , P = 0 01 ) . -
pubmed
It shows that a non - irradiation and
busulfan - free conditioning regimen can be used successfully , also in alternative donor
SCT . -
pubmed
18F - FDG PET /
CT of Seminal Vesicle Metastasis From Ascending Colon
Adenocarcinoma . -
pubmed
This also highlights the role of
F - FDG PET /
CT to detect metastatic
adenocarcinoma from colon in the genitourinary system . -
pubmed
Patients were genotyped for functional variants on
vitamin D synthetic pathway including
GC ( rs4588 , rs7041 , rs22020 , rs2282679 ) ,
CYP2R1 ( rs2060793 , rs12794714 ) ,
CYP27B1 ( rs10877012 ) , and
DHCR7 ( rs12785878 ) . -
pubmed
Mutation analysis of
SUOX revealed homozygous c.1200C & gt ; G mutations , resulting in an
amino acid substitution of
tyrosine to a stop codon ( Y400X ) . -
pubmed
The completely demineralized dentine
collagen blocks were then remineralized in a
calcium chloride agarose hydrogel and a
sodium hydrogen phosphate and
fluoride agarose hydrogel . -
pubmed
Starting from common commercially available materials , the titled
cis / trans - tetraarylethenes can be conveniently obtained by a one -
pot process under mild conditions . -
pubmed
Developing
bNAbs with increased efficacy requires understanding how antibodies interact with the native
oligomannose and complex - type N - glycan shield that hides most protein epitopes on HIV-1 envelope (
Env ) . -
pubmed
Dorsal repulsive axon guidance protein (
Draxin ) is a novel guidance molecule that binds not only common guidance molecule receptors with
Netrin-1 , but also directly binds the
EGF domain of
Netrin-1 through a
22 - amino - acid peptide ( 22aa ) . -
pubmed
, past-30 - day
cocaine use and intravenous opiate use ) , number of positive drug tests , and receiving any sanction / therapeutic response were associated with two - year
post - program recidivism . -
pubmed
We report here that
VB1 promotes the protective immune response to limit the survival of
MTB within macrophages and in vivo through regulation of
peroxisome proliferator - activated receptor gamma (
PPAR - gamma ) . -
pubmed
In addition ,
VB1 increases mitochondrial respiration and lipid metabolism and
PPAR - gamma integrates the metabolic and inflammatory signals regulated by
VB1 . -
pubmed
Limonene Inhibits
Methamphetamine - Induced Sensitizations via the Regulation of
Dopamine Receptor Supersensitivity . -
pubmed
These results suggest that
limonene may ameliorate
drug addiction - related behaviors by regulating postsynaptic
dopamine receptor supersensitivity . -
pubmed
The crystallite size and band gap values of
SiO2 ,
TiO2 ,
NiS -
SiO2 and
Cr2S3 -
TiO2 - 1 were obtained from XRD and UV - vis
DRS analysis and found insignificant 44.22 , 54.11 , and 57.11 nm , and 8.9 , 3.2 , 3.0 , 2.7 eV , respectively . -
pubmed
Folic acid (
FA ) is one of the biologic molecules which has been targeted overexpressed -
folic acid receptor (
FR ) on the surface of
cancer cells . -
pubmed
Blockage of
PAR-1 with the
PAR-1 - specific antagonist ,
SCH79797 , significantly suppressed the activation of
Rab5 ,
EEA-1 , and the
ERK1 / 2 and
AKT pathways and subsequently increased EV production and enriched EV cargo contents . -
pubmed
The genes
gyrA ,
gyrB ,
rrs ,
eis promoter and
tlyA of 257 drug - resistant clinical isolates were sequenced to identify mutations that could be responsible for resistance against
fluoroquinolones and second - line injectable drugs . -
pubmed
Mechanistic study revealed that
SAL - miat acted as a competitive endogenous RNA ( ceRNA ) that upregulated the expression of
sirt1 by sponging miR-22 - 3p . -
pubmed
Stress and motivation status after rehabilitation were determined by
corticosterone concentrations ( ELISA ) and immunoreactivity of
DeltaFosB ( immunohistochemistry ) in the nucleus accumbens , respectively . -
pubmed
To investigate the motivation and stress related to the exercises , the expression of
DeltaFosB in the nucleus accumbens and
corticosterone concentration were compared after rehabilitation . -
pubmed
Finally , living imaging and
hematoxylin and
eosin (
HE ) staining were used to measure the morphology and pathology of lung and
liver cancer cell
metastases , respectively . -
pubmed
Our group previously demonstrated that
sarcoma cell lines were insensitive to
epidermal growth factor receptor (
EGFR ) inhibitor
gefitinib monotherapy . -
pubmed
Considering the positive regulation of
tumour energy production by both the
EGFR signalling and
tumour metabolism pathways , this study aimed to investigate the effect and mechanisms of combination therapy using
gefitinib and
PENAO in
sarcoma cell lines in vitro and in vivo . -
pubmed
In addition , with the increase of the introduced
B3 monomer , the oxidation stability of
SPI-6FODA has been greatly improved . -
pubmed
Herein , pore size controllable meso -
TiO2 layers are prepared using spin coating of commercial
TiO2 nanoparticle (
NP ) paste with added soft polymer templates ( SPT ) followed by removal of the SPT at 500 C. The SPTs consist of swollen crosslinked polymer colloids ( microgels , MGs ) or a commercial linear polymer ( denoted as LIN ) . -
pubmed
The
PDGF - BB - induced stimulation of
Kir2.1 currents was blocked by the
PDGF - BB receptor beta (
PDGF - BBRbeta ) inhibitor
AG1295 and was not affected by the
PDGF - BBRalpha inhibitor
AG1296 . -
pubmed
The
PDGF - BB - induced stimulation of
Kir2.1 currents was blocked by the
protein kinase A inhibitor
Rp-8 - CPT - cAMPs ; however , the antagonist of
protein kinase B (
GSK690693 ) had marginal effects on current activity . -
pubmed
The
PDGF - BB - induced stimulation of
Kir2.1 currents was enhanced by
forskolin , an
adenylyl cyclase (
AC ) activator , and was blocked by the AC inhibitor
SQ22536 . -
pubmed
We conclude that
PDGF - BB increases
Kir2.1 currents via
PDGF - BBRbeta through activation of
cAMP -
PKA signaling in RASMCs . -
pubmed
Fluctuation of
sulfite reductase (
SiR ) activity , expression of
MET5 and
MET10 genes , and concentrations of
sulfite and
selenium were evaluated to support the population findings . -
pubmed
A novel strategy to treat
CADM with rapidly progressive
interstitial lung disease is the
Janus kinase inhibitor
tofacitinib . -
pubmed
Chronic
arsenic exposure enhances metastatic potential via
NRF2 - mediated upregulation of
SOX9 . -
pubmed
One proposed contributor to
metastasis in
NSCLC is
nuclear factor ( erythroid - derived 2 )- like 2 (
NRF2 ) , a transcription factor with known oncogenic properties that has proved to be critical for
arsenic carcinogenesis . -
pubmed
Here , we demonstrate that chronic
arsenic exposure enhances the invasive and migratory capacity of immortalized lung epithelial cells via
NRF2 - dependent upregulation of
SRY - box 9 (
SOX9 ) , another transcription factor linked with cell proliferation , epithelial - mesenchymal transition , and
metastasis . -
pubmed
MYCNOS1 silencing enhanced the response of
retinoblastoma cells to
topotecan but not
carboplatin . -
pubmed
Experiments were repeated with
AS101 ( an
IL-10 protein synthesis inhibitor ) ,
MS-275 ( a selective class 1
HDAC inhibitor ) , or both . -
pubmed
Increase of the
sialic acid - rich variants of the B beta- and gamma - chains of
fibrinogen Zurich II . -
pubmed
The effects of
saralasin , an
angiotensin II antagonist , on blood pressure and the
renin -
angiotensin -
aldosterone system in normal and
hypertensive subjects . -
pubmed
Radioligand binding to forebrain and hindbrain membranes after 6 days treatment was examined using
[ 3H ] yohimbine to measure the number of
adrenergic binding sites and [
3H]
clonidine and
[ 3H ] idazoxan to assess nonadrenergic
imidazoline sites . -
pubmed
At pH 7.25 ,
CqTX is as potent as
TTX in blocking the
sodium channel , with an ED50 of 3.8 nM. -
pubmed
Platelet activating factor (
PAF ) is a
phospholipid mediator of
inflammation and vascular leakage that may be important in the etiology of
asthma . -
pubmed
PAF - induced vascular leakage was inhibited in a dose - dependent fashion by the
PAF receptor blocker
WEB 2086 . -
pubmed
However ,
PAF - induced vascular leakage was not inhibited by blockade of
cyclooxygenase /
lipoxygenase ,
calmodulin ,
calcium channels ,
protein kinase C ,
histamine receptors , or by destruction of peptidergic sensory nerves . -
pubmed
Recently developed
monoclonal antibodies against V gamma and V
delta gene products are useful
reagents for the identification and isolation of gamma / delta+ T - cell subpopulations . -
pubmed
It is suggested that an endogenous
BR ligand down - regulating
GABAA receptors is released during the
bicuculline - evoked enhancement of neuronal activity and may contribute to the epileptiform discharge of motoneurons in the spinal cord . -
pubmed
The purpose of this study was to determine ( a ) if the administration of
DHT suppresses
plasma prolactin levels or its nocturnal surge within 24 h after the treatment , ( b ) how soon after the commencement of treatment do the concentrations of
DHT increase and
progesterone levels decrease in the circulation , ( c ) the ultrastructural changes that occur in corpora lutea , and ( d ) the changes in
luteal P-450 side - chain cleavage (
P-450scc ) enzyme and mRNA content upon
DHT treatment . -
pubmed
ESR spectra of spin - labelled
cholestane (
CSL ) revealed that this
steroid probe was incorporated into the
AChR - rich membrane vesicles in regions which were to a certain extent enriched preferentially in the
steroid , both in the presence and in the absence of local anaesthetics . -
pubmed
Since the
nitroxide group present in
CSL is also a paramagnetic quencher of the intrinsic protein fluorescence , this property was used to characterize the
AChR -
steroid interactions . -
pubmed
The quenching induced by
CSL was sensitive both to
AChR concentration and to the action of cholinergic agonists . -
pubmed
In competition experiments , the ability of
CSL to quench the
AChR intrinsic fluorescence was markedly inhibited by
benzocaine ,
tetracaine and
QX-222 ( a
quaternary trimethylammonium derivative of
lidocaine ) , and was totally inhibited by
procaine . -
pubmed
The effectiveness of local anaesthetics in inhibiting
CSL - induced quenching followed the order :
procaine much greater than
benzocaine approximately greater than
tetracaine greater than
QX-222 . -
pubmed
The data can be interpreted in terms of a model requiring specific association sites for local anaesthetics on the hydrophobic surface of the
AChR which at least partially overlap with those for
steroids . -
pubmed
After
IV administration ,
ketamine kinetics followed a 3 - term exponential decrease , indicating rapid initial distribution of the drug to highly vascular tissues including the brain , followed by redistribution into less vascular tissues , and elimination . -
pubmed
The synthesis and testing of
E-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone and
Z-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone as gamma - emitting ligands for the
androgen receptor . -
pubmed
Two
iodinated steroids ,
E-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone and
Z-17 alpha -( 2 - iodovinyl )- 5 alpha - dihydrotestosterone were synthesized in a search for a gamma - emitting
androgen that binds with high affinity to the
androgen receptor . -
pubmed
These
17 alpha - iodovinyl steroids were synthesized because many 17 alpha - substituents do not interfere markedly with binding to the
androgen receptor and because similar analogs of other
steroids ,
estrogens and
progestins , have been shown to have the requisite properties for ligands to those receptors . -
pubmed
Both of these potential ligands were tested for their ability to compete with
[ 3H ] R1881 for binding to the
androgen receptor in cytosols from prostate , hypothalamus and pituitary . -
pubmed
In order to test the two ligands directly , they were both synthesized labelled with
125I and tested for binding to the
androgen receptor in prostatic cytosol and in vivo for specific concentration in
androgen responsive tissues . -
pubmed
While on the basis of relative binding affinity , these 2
steroids appeared to be good candidates for
androgen receptor ligands , neither were useful for this purpose . -
pubmed
Although no specific
gpNu1 binding to the left side of
cos can be detected , this DNA segment does play a specific role since a cos fragment that does not include the left side or whose left side is replaced by non - cos sequences , is unable to bind
gpNu1 unless either
spermidine or
IHF is present . -
pubmed
Reduction of
catalase by feeding
3 amino 1,2,4 triazole does not enhance the
toxicity of
phosphine in treated insects . -
pubmed
In pharmacologic doses ( 1 g or more / day ) , alone or in combination with other lipid - lowering drugs ,
nicotinic acid lowers very low - density (
VLDL ) and
low - density lipoprotein (
LDL ) levels , while concurrently increasing
high - density lipoprotein (
HDL ) levels . -
pubmed
Mass spectrometry of
L - beta - aspartamido carbohydrates isolated from
ovalbumin . -
pubmed
In the process , we have discovered six new components in the
L - beta - aspartamido carbohydrate fraction of
ovalbumin . -
pubmed
The
bilirubin values were low in the control group and after selective proximal vagotomy without pyloroplasty , higher after selective proximal vagotomy with Finney 's operation , antecolic Billroth
II operation with enteroanastomosis by Baum and Billroth I operation , maximally after retro- and antecolic Billroth
II operation . -
pubmed
Influence of metabolic inhibitors on
ATP level and
ATP - ase activity in blood platelets . -
pubmed
All the data indicate that increased gluconeogenesis is an early response to metabolic
acidosis and will facilitate
ammonia production by utilization of
glutamate which inhibits the
glutaminase I enzyme . -
pubmed
Evaluation of the reliability of
hemoglobin determination by
copper sulphate densitometry . -
pubmed
More severe
vena caval obstruction ( n = 10 ) decreased mean arterial pressure 13 + /- 2 mmHg , central venous pressure 1.3 + /- 0.3 mmHg , and heart rate 47 + /- 12 beats / min , and increased fetal plasma
ACTH 1,047 + /- 448 pg / ml ,
cortisol 4.4 + /- 2.2 ng / ml , and
vasopressin 47.9 + /- 24.2 pg / ml , but did not alter
11 - deoxycortisol . -
pubmed
End - inflation plasma
ACTH and
vasopressin concentrations were significantly related to the induced
decreases in mean arterial and central venous pressures , suggesting that the hormonal responses to
vena caval obstruction were mediated by cardiovascular mechanoreceptors . -
pubmed
By analogy with other
steroid hormones , the production of better , and especially more specific ,
antibodies might be expected . -
pubmed
This stimulation was most powerful at low concentrations of
enzyme , or high concentrations of
squalene . -
pubmed
Epoxidase activity was destroyed by
Triton X-100 ,
deoxycholate or
Cu2 + , and partially inhibited by
thiol reagents ,
rotenone and
antimycin A. -
pubmed
The
enzyme was not inhibited by
cyanide or by several inhibitors of
cytochrome P-450 . -
pubmed
A study has been made of the
IMP or
poly ( I ) interactions leading to substitution of X and Y ligands in binuclear
platinum complexes of the general formula :
[ X - Pt ( dien )] m +-( CH2 ) 6 -[( dien ) Pt - Y ] n + ( NO3 ) m + n , where
dien stands for
diethylenetriamine , X =
H2O , Y =
Br , m = 2 , n = 1 ( I ) , X = Y =
H2O , m = n = 2 (
II ) , and X = Y =
Br , m = n = 1 ( III ) . -
pubmed
PVA had no effect on the responsiveness to
phytohemagglutinin - P , whereas HB and BV enhanced the
phytohemagglutinin - P responsiveness . -
pubmed
It has been suggested that an inhibitory ECM containing
chondroitin-6 - sulfate proteoglycan (
C-6S - PG ) and
tenascin (
TN ) , which appears homogeneously in the core of the
OB following afferent fiber arrival , helps position ingrowing olfactory axons in the prospective glomerular layer (
GL ) ( Gonzalez and Silver , 1992 ; Gonzalez et al . -
pubmed
In the present study , we suggest that the formation of
TN /
C-6S - PG in the precise ring pattern around glomeruli appears to be dependent upon the presence of bundled olfactory axons . -
pubmed
Significant proliferation was also induced by
IL-6 ( 7 + /- 2.3 % of BRDU+ cells ; P = 0.006 ) ,
IL-3 ( 5.3 + /- 1.3 % ; P = 0.01 ) and
PIXY-321 ( 5.4 + /- 1.6 % ; P = 0.02 ) . -
pubmed
We have investigated the mechanisms involved in this
cAMP - mediated regulation of
c - myc expression . -
pubmed
These data suggest that
cyclic AMP downregulates
c - myc expression by inhibition of transcriptional initiation . -
pubmed
The major cytosolic
phospholipase A2 isoform preferentially hydrolyzes
plasmalogen substrate , possesses a pH optimum of 7.0 , and is chromatographically resolvable from a minor cytosolic
calcium - independent
phospholipase A2 isoform that hydrolyzes
plasmenylcholine and
phosphatidylcholine substrates at similar rates and possesses a pH optimum of 8.5 . -
pubmed
The major cytosolic
calcium - independent
phospholipase A2 isoform was identified as a 40 - kD polypeptide after its 182,000 - fold purification by sequential column chromatographies to a final specific activity of 67 mumol / mg.min . -
pubmed
The specific role of
sterol carrier protein-2 (
SCP-2 ) in the transport of newly synthesized biliary
cholesterol was evaluated by an in vivo antisense
oligonucleotide approach . -
pubmed
In vivo
SCP-2 antisense treatment reduced and delayed the appearance of biliary
[ 14C ] cholesterol . -
pubmed
These results suggest that
SCP-2 is necessary for the rapid transport of newly synthesized
cholesterol into bile and that hepatocytes can induce
SCP-2 expression according to the rate of biliary secretion of newly synthesized
cholesterol . -
pubmed
Cultured
tumor cells released
PTH and were
calcium sensitive , producing 122 + /- 16 pg / microgram DNA of intact
PTH ( mean + /- SEM ) at 0.5 mmol / L
calcium compared with 26 + /- 2 pg / microgram DNA at 3.0 mmol / L
calcium . -
pubmed
However , we found that
somatostatin ( 0.1 microgram / L ) in cultured
tumor cells increased the release of intact
PTH ( 123 + /- 19 versus 82 + /- 1 pg / microgram DNA , P & lt ; 0.05 ) and thus might have a negative effect on the
HHM . -
pubmed
The stereospecific enzymatic hydrolysis by an array of commercially available
enzymes of the racemic
methyl ester of
LLU - alpha was investigated . -
pubmed
Four hundred forty - two women and 341 men were surveyed at Panama City Beach , Florida , to assess the effects of gender , age , fraternity or sorority membership , and travel motivation on
alcohol consumption and binge drinking during
spring break . -
pubmed
Under constant - flow conditions , intraarterial injections of
endothelin-1 and
sarafotoxin 6c induced biphasic changes in hind - limb perfusion pressure characterized by an initial decrease followed by a secondary increase in perfusion pressure . -
pubmed
The vasodilator and vasoconstrictor components of the biphasic responses to
endothelin-1 and sarafotoxin 6c were reduced by
bosentan , and the
endothelin receptor antagonist reduced baseline systemic arterial and hind - limb perfusion pressures . -
pubmed
Bosentan decreased vasoconstrictor responses to lower doses of
angiotensin II , whereas responses to higher doses of
angiotensin II and responses to
vasopressin ,
U46619 ,
BAY K8644 ,
norepinephrine ,
acetylcholine ,
bradykinin ,
levcromakalim ,
PGE1 ,
adrenomedullin , and
calcitonin gene - related peptide were not altered . -
pubmed
Vasoconstrictor responses to
ET-1 were not altered by the
angiotensin AT1 receptor antagonist
DuP 532 or the
AT2 receptor antagonist
PD123,319 . -
pubmed
The
protein tyrosine kinase could phosphorylate exogenous substrate corresponding to the N - terminal peptide of
p34cdc2 kinase but could not do so on
poly ( Glu : Tyr ) . -
pubmed
The
enzyme could not be inhibited by
genistein ,
staurosporine and
dimethyl aminopurine , but could be inhibited by
piceatannol which is a known competitive inhibitor of the peptide binding site of
mammalian protein tyrosine kinases . -
pubmed
The
enzyme activity in the cytoplasm is uniquely inhibited by
sodium orthovanadate ( IC50 = 20 microM ) which is a known
protein tyrosine phosphatase inhibitor . -
pubmed
Regenerating hepatocytes after partial hepatectomy induced
MGMT transiently within 3 days ; however , the induction of
MGMT was persistent for 2 weeks after
DEN injection , and the combined treatment of
DEN and partial hepatectomy maintained the elevated
MGMT level for up to 4 weeks . -
pubmed
The biological significance of the differential induction of
MGMT during partial hepatectomy or
DEN - induced
carcinogenesis is not obvious ; however , further studies on possible posttranslational modifications of
MGMT protein might shed some light on the functional aspect of
MGMT induction . -
pubmed
Comparison of the effects of DEPs , extracted DEPs , or extracts of DEPs has shown that the increase in
GM - CSF release is mainly due to the adsorbed organic compounds and not to the metals present on the DEP surface because the metal chelator
desferrioxamine had no inhibitory effect . -
pubmed
Furthermore , radical scavengers inhibited the DEP - induced
GM - CSF release , showing involvement of reactive
oxygen species in this response . -
pubmed
Moreover
genistein , a
tyrosine kinase inhibitor , abrogated the effects of DEPs on
GM - CSF release , whereas
protein kinase ( PK ) C ,
PKA ,
cyclooxygenase , or
lipoxygenase inhibitors had no effect . -
pubmed
PD-98059 , an inhibitor of
mitogen - activated protein kinase , diminished the effects of DEPs , whereas
SB-203580 , an inhibitor of
p38 mitogen - activated protein kinase , had a lower effect , and DEPs did actually increase the active , phosphorylated form of the
extracellular signal - regulated kinase as shown by Western blotting . -
pubmed
In addition ,
cytochalasin D , which inhibits the phagocytosis of DEPs , reduced the increase in
GM - CSF release after DEP treatment . -
pubmed
Reactive
oxygen species and
tyrosine kinase(s ) may be involved in the DEP - triggered signaling of the
GM - CSF response . -
pubmed
The demonstration of the presence of the
elastin receptor on leukocytes facilitated the detailed description of the transmission pathway from receptor to the intracellular sites activated by the receptor : modifications of ion fluxes , increase of
elastase production and excretion of reactive
oxygen species ,
superoxide and
NO * . -
pubmed
The
calcium transients triggered by
elastin peptides acting on the receptor decrease with age , the receptor appears to be uncoupled from the
G - proteins , but
superoxide release is increased . -
pubmed
The reactions required
NADPH as a cofactor and were significantly inhibited by the various inhibitors of
cytochrome P450 ( i.e. -
pubmed
The results indicate that the degraded products of
OPB-2045 are produced by
C - C bond cleavage after monohydroxylation , dihydroxylation , and
ketol formation at the site of the
octyl side chain with possible involvement of
cytochrome P450 systems . -
pubmed
The two
URA3 alleles were sequentially inactivated using the MPA(R)-flipping strategy , which is based on the selection of integrative transformants carrying a
mycophenolic acid (
MPA ) resistance marker that is subsequently deleted again by site - specific ,
FLP - mediated recombination . -
pubmed
When
MPA(R ) was used as a reporter gene , it conferred an
MPA - resistant phenotype on opaque but not white cells in
strains expressing it from the
SAP1 promoter , which was monitored at the level of single cells by a significantly enlarged size of the corresponding colonies on
MPA - containing indicator plates . -
pubmed
Similarly , white but not opaque cells became
MPA resistant when
MPA(R ) was placed under the control of the
WH11 promoter . -
pubmed
Signal transduction from
RAGE to
NF - kappaB involved the generation of reactive
oxygen species , since reporter gene expression was blocked with the antioxidant
N - acetyl - L - cysteine . -
pubmed
CML - modified albumin produced rapid transient activation of
tyrosine phosphorylation ,
extracellular signal - regulated kinase 1 and 2 , and
p38 mitogen - activated protein kinase (
MAPK ) , but not
c - Jun NH ( 2 )- terminal kinase . -
pubmed
RAGE - mediated
NF - kappaB activation was suppressed by the selective
p38 MAPK inhibitor
SB203580 and by coexpression of a
kinase - dead
p38 dominant - negative mutant . -
pubmed
The
PKC inhibitors
staurosporine and
calphostin C inhibited the increase in force/[
Ca ( 2 +)]i induced by
phenylephrine to a much greater extent than that induced by 80 mM
K + . -
pubmed
LY379196 , a specific
PKCbeta inhibitor , did not inhibit the increase of
calcium sensitivity due to
phenylephrine . -
pubmed
Whole - mount
X - gal staining of 9.5 dpc embryos indicated Cre - mediated
lacZ expression in forebrain , hindbrain , and along the midbrain flexure . -
pubmed
Serum and liver samples obtained from dams ( GD 20 and
PND 22 ) , fetuses ( GD 20 ) , and offspring ( PNDs 4 , 14 , 36 , and 90 ) were analyzed for circulating total serum
thyroxine ( T(4 ) ) and
triiodothyronine ( T(3 ) ) , or hepatic microsomal
ethoxy - and pentoxy - resorufin - O - deethylase (
EROD and
PROD ) , and
uridine diphosphoglucuronosyl transferase (
UDPGT ) activity . -
pubmed
Induction of PROD was similar in both dams and offspring ; however ,
EROD and
UDPGT induction were much greater in offspring compared to dams ( EROD = 3.8 - fold ;
UDPGT = 0.5 - fold ) . -
pubmed
Immunoglobulin G - class mouse monoclonal antibodies to major brain
gangliosides . -
pubmed
Enzyme - linked immunosorbent assay ( ELISA ) screening against 14 closely related synthetic and purified
gangliosides confirmed the high specificity , with no significant cross - reactivity except that of the
anti - GD1a mAbs for the closely related minor
ganglioside GT1a alpha . -
pubmed
Lysine 270 in the third intracellular domain of the
oxytocin receptor is an important determinant for
G alpha ( q ) coupling specificity . -
pubmed
Substitution of the 2i domain of
OTR into V(2)R decreased ligand affinity and
vasopressin - stimulated
adenylyl cyclase activity and only slightly increased
phosphatidylinositide turnover . -
pubmed
In contrast , substitution of the OTR3i domain into V(2)R produced a receptor chimera with high ligand affinity , decreased
vasopressin - stimulated
adenylyl cyclase activity , and markedly enhanced ligand - stimulated
phosphatidylinositide turnover . -
pubmed
The C - terminal 36
amino acids , but not the N - terminal 13
amino acids , of the OTR3i domain contained the determinants critical for enhanced activation of
PLC . -
pubmed
Mutation of a single
lysine in the C - terminal OTR3i sequence to the corresponding V(2)R residue (
valine ) eliminated the enhanced ability of the V(2)R chimera to stimulate
PLC but did not affect maximal
adenylyl cyclase stimulation . -
pubmed
Furthermore , mutation of this residue ( K270 ) in wild - type
OTR completely abolished the ability of the receptor to stimulate
phosphatidylinositide turnover , with only a small reduction in ligand affinity . -
pubmed
These data demonstrate that
OTR K270 is critically important in the stimulation by
OTR of
phosphatidylinositide turnover and that this determinant can also increase this activity in the V(2)R chimera . -
pubmed
A pH dependence study on the unfolding and refolding of
apoazurin : comparison with
Zn ( II ) azurin . -
pubmed
Previous folding studies have shown that the equilibrium denaturation of the
holoprotein follows a two - state process ; however , upon removal of the
copper , the denaturation had been reported to follow a three - state process . -
pubmed
It is our contention that the second transition displayed in the denaturation curves previously reported for
apoazurin arise from protein heterogeneity - in particular , from the presence of
Zn ( II ) azurin . -
pubmed
We extended our investigation into the denaturation of
Zn ( II ) azurin at pH 6.0 and 7.5 . -
pubmed
Further , comparing the denaturation curves of
Zn ( II ) azurin measured by circular dichroism with those measured by fluorescence indicates that the denaturation of
Zn ( II ) azurin is far less simple than the denaturation of
apoazurin . -
pubmed
Gastric luminal and
serum gastrin levels and gastric mucosa and
tumor PGE2 , which were greatly elevated before eradication , became normalized after this procedure . -
pubmed
It shares less than 20 %
amino acid sequence identity with other
lipases for which there are crystal structures , and shows significant insertions compared with the typical
alpha / beta hydrolase canonical fold . -
pubmed
The structure contains a
zinc - binding site which is unique among all
lipases with known structures , and which may play a role in enhancing thermal stability . -
pubmed
In contrast ,
alpha - CT did not potentiate the twitch response or contractions induced respectively by a single
pulse TNS or by direct electrical stimulation to the smooth muscle . -
pubmed
The regimen outlined in this brief paper focuses on therapeutically utilizing
iron and
iron compounds in
transferrin receptor - rich
tumor cells in ways that inhibit and lyse same . -
pubmed
Balanol analogues probe specificity determinants and the conformational malleability of the
cyclic 3 ' , 5 '- adenosine monophosphate - dependent protein kinase catalytic subunit . -
pubmed
Here , we characterize three
balanol analogues that inhibit
cyclic 3 ' , 5 '- adenosine monophosphate - dependent protein kinase (
PKA ) more specifically and potently than
calcium - and phospholipid - dependent protein kinase (
PKC ) . -
pubmed
Crystal structures of the
PKA catalytic ( C ) subunit complexed to each analogue show the
Gly - rich loop stabilized in an " intermediate " conformation , disengaged from important
phosphoryl transfer residues . -
pubmed
The
balanol analogues serve to test the model and to highlight differences in the active site local environment of
PKA and
PKC . -
pubmed
The
PKA C subunit appears to tolerate
balanol analogues with D - ring modifications ;
PKC does not . -
pubmed
A deglucosylated metabolite of
paeoniflorin ,
paeoniflorgenin ( PG ) , in serum was identified based on HPLC /
MS and NMR spectral data . -
pubmed
Membrane microdomains enriched in glycosphingolipids ,
cholesterol ,
glycosylphosphatidylinositol - anchored proteins and
Src - family kinases ( lipid rafts , GEMs ) appear to play many important roles , especially in immunoreceptor signaling . -
pubmed
All these molecules possess a very short N - terminal extracellular peptide ( 4 - 17
amino acids ) , transmembrane segment followed by a palmitoylation motif ( CxxC ) and cytoplasmic domain containing up to 10
tyrosine residues potentially phosphorylated by the
Src- or
Syk - family kinases . -
pubmed
Tyrosine - phosphorylated transmembrane adaptors bind ( directly via
SH2 domains or indirectly ) other
signaling molecules such as several
cytoplasmic adaptors and enzymes . -
pubmed
The number of
AR - immunoreactive (
AR - ir ) cells was regulated by
testosterone in medial amygdala and caudal arcuate , and by photoperiod in the medial preoptic nucleus and the posterodorsal medial amygdala . -
pubmed
A significant interaction between
androgen treatment and daylength in regulation of
SRC-1 - ir was found in anterior medial amygdala . -
pubmed
The present results indicate that daylength - induced fluctuations in
SRC-1 and
AR expression may contribute to seasonally changing effects of
testosterone . -
pubmed
The total increment of plasma
homocysteine was assessed using the positive incremental area under the plasma
homocysteine concentration curve over the 240 - minute
post -
methionine - loading period ( DeltaAUC(0 - 240 ) ) . -
pubmed
Postoperatively , they exhibited decreased levels of
interleukin ( IL )- 2 ,
interferon - gamma ,
IL-12 ,
granulocyte - macrophage colony - stimulating factor and
IL-15 , low cellular immune responses ( AMLR , NK- and
LAK -
cytotoxicity ) and increased levels of
IL-1beta ,
tumor necrosis factor - alpha ,
IL-6 ,
IL-10 and
prostaglandin E ( 2 ) . -
pubmed
Molecular basis of the low activity of antitumor
anthracenediones ,
mitoxantrone and
ametantrone , in
oxygen radical generation catalyzed by
NADH dehydrogenase . -
pubmed
Synthetic antitumor
anthracenedione drugs , in contrast to
anthracycline antibiotics , are ineffective in free radical formation in
NADH dehydrogenase system . -
pubmed
Our results have indicated that neither the reduction potential nor the side chain conformation and the energies of border orbitals ( HOMO and LUMO ) determine the ability of
anthracenediones to stimulate reactive
oxygen species formation in
NADH dehydrogenase system . -
pubmed
It was shown that the distribution of the molecular electrostatic potential (
MEP ) , around the
quinone system was crucial for this ability . -
pubmed
We have found for non - stimulating
anthracenediones that the clouds of positive
MEP cover the
quinone carbon atoms while for agents effective in stimulating reactive
oxygen species formation the clouds of negative
MEP cover continuously the aromatic core together with the
quinone system . -
pubmed
Among them , eight are new , namely as
trichobrachin A I - IV (
Aib9 - Pro10 sequence ) and as trichobrachin B I -
IV ( Val9 - Pro10 sequence ) . -
pubmed
However , we provide evidence that a non - autonomous increase of
Diap1 protein levels , an
inhibitor of apoptosis , accounts for the suppression of
hid . -
pubmed
Furthermore , before they die ,
vps25 mutant clones trigger non - autonomous proliferation through a failure to downregulate Notch signaling , which activates the
mitogenic JAK /
STAT pathway . -
pubmed
After osmotic
rupture of the isolated amyloplasts and
chloroplasts , a clear separation of the envelope - membranes was achieved by discontinuous
sucrose density gradient centrifugation . -
pubmed
BCR /
ABL - positive
leukemia cells accumulated more replication - dependent DNA double - strand breaks ( DSBs ) than normal counterparts after treatment with
cisplatin and
mitomycin C (
MMC , as assessed by
pulse field gel electrophoresis ( PFGE ) and neutral comet assay . -
pubmed
Elevated levels of drug - induced DSBs in
leukemia cells were associated with higher activity of
ATR kinase , and enhanced phosphorylation of
histone H2AX on
serine 139 (
gamma - H2AX ) . -
pubmed
In addition , the expression and
ATR - mediated phosphorylation of
Chk1 kinase on
serine 345 were often more abundant in
BCR /
ABL - positive
leukemia cells than normal counterparts after genotoxic treatment . -
pubmed
Inhibition of
ATR kinase by
caffeine but not
Chk1 kinase by
indolocarbazole inhibitor ,
SB218078 sensitized
BCR /
ABL leukemia cells to
MMC in a short - term survival assay . -
pubmed
i.p ) enhanced lipid peroxidation ,
hydrogen peroxide content ,
glutathione S - transferase and
xanthine oxidase with reduction in the activities of hepatic antioxidant enzymes
viz . -
pubmed
,
glutathione peroxidase ,
superoxide dismutase and caused depletion in the level of hepatic
glutathione content . -
pubmed
TAA treatment also enhanced
tumor promotion markers ,
ornithine decarboxylase (
ODC ) activity and
[ 3H ] thymidine incorporation into hepatic DNA . -
pubmed
Spatial variations in electric conductivity and evolutions of band structures of
polyaniline (
PANI ) films have been studied by use of a so - called current - sensing atomic force microscope (
CS -
AFM ) or atomic force microscope current image tunneling spectroscopy (
AFM - CITS ) . -
pubmed
Successful callus formation was obtained in all three species using Murashige - Skoog 's (
MS ) medium supplemented with
1 - naphthalene - acetic acid (
NAA ) ( 1 ppm ) and
kinetin ( 0.1 ppm ) . -
pubmed
Plantlet formation of C. ZEDOARIA from stem tips was successfully conducted on
MS medium containing
NAA ( 0 - 1 ppm ) or
6 - benzyladenine ( BA)(0 - 3 ppm ) at 26 degrees C under the condition of 12 h photoperiod . -
pubmed
Exercise increases the distribution of
leukotrienes and influences the
leukotriene transcription pathway ; it could be shown that the genes
ALOX5 and
ALOX5AP encoding for
5 - lipooxygenase ( 5 - LO ) and
5 - lipoxygenase - activating protein (
FLAP ) as well as activators for
5 - LO ,
p38 - mitogen - activated protein kinase (
MAPK ) , and others , are enhanced after exercise in healthy subjects . -
pubmed
Possibly
EIA is triggered via
leukotriene release if a predisposition or other conditions ( eg ,
epithelial injury and repair ) are present . -
pubmed
Binding of the secreted axon guidance cue
Slit to its
Robo receptor results in inactivation of the neural ,
calcium - dependent cell - cell adhesion molecule
N - cadherin , providing a rapid epigenetic mechanism for integrating guidance and adhesion information . -
pubmed
Complex formation results in
Abl - mediated phosphorylation of
beta - catenin on
tyrosine 489 , leading to a decrease in its affinity for
N - cadherin , loss of
N - cadherin function , and targeting of
phospho - Y489 -
beta - catenin to the nucleus . -
pubmed
In addition , seeds of this and other mutants in the
ethylene signaling pathway (
ctr1 - 1 ,
eto1 - 1 ,
etr1 - 1 ,
ein2 - 1 ) show different response to imbibition than the wild type . -
pubmed
In contrast , in the seeds of the
ethylene mutants , reduction in curvature values occurs in both basal and apical
poles , and its shape remains as a prolate spheroid . -
pubmed
Our data indicate that the
ethylene signaling pathway is involved , in general , in the complex regulation of seed shape and , in particular , in the establishment of polarity in seeds , controlling curvature values in the seed
poles . -
pubmed
Exploratory trials are investigating the use of more complete
estrogen suppression with
luteinizing - hormone - releasing hormone agonists with
aromatase inhibitors . -
pubmed
In addition to these
granuphilin - null phenotypes ,
Rab27a /
granuphilin doubly deficient cells showed the decreases in granules located next to the docked area and in fusion from granules near the plasma membrane in the early phase of
glucose - stimulated secretion , similar to
Rab27a - mutated cells . -
pubmed
Activin inhibitors (
Activin - M108A /
SB431542 ) with differing specificities for the other
TGFbeta members under consideration were applied during HESC decidualization in vitro . -
pubmed
Spotlight on anti -
CD25 :
daclizumab in
MS . -
pubmed
One of these , the humanized monoclonal anti -
CD25 antibody
daclizumab (
Zenapax ) , is directed against the
interleukin-2 ( IL-2 ) receptor alpha chain (
CD25 ) that is involved in clonal expansion of autoreactive T - cells by binding of its ligand
IL - 2 . -
pubmed
Following promising observations in
uveitis ,
daclizumab has since been tested in a number of small clinical trials in
MS based on the rationale that blocking
CD25 would prevent the expansion of autoreactive T - lymphocytes . -
pubmed
The chemoresistive sensors based on single
poly ( pyrrole )- NTA chelator nanotube exhibited detection as low as one - hundredth attomolar ( 0.6 ppt )
cupric ions and 1 ng / ml of
penta - histidine tagged
syntaxin protein . -
pubmed
C18 , C8 , and
perfluoro reversed phases on
diamond for solid - phase extraction . -
pubmed
At the highest dosages used ,
FOA -
PYR and
FOA -
DAP -
ART combinations were synergistic with 100 % cure rate , while
FOA - PYR - ART was antagonistic . -
pubmed
[ Synthesis and
alpha - glucosidase inhibitory activity of
N -( 1,5 - diaryl-3 - pentone-1 - yl )- 4 - aminobenzoic acid ] . -
pubmed
The
MP structural gene (
lupA gene ) was cloned by inverse PCR , and the open reading frame of 1,443 bp encoded a 463
amino acid protein ( without signal peptide ) . -
pubmed
Phosphatidylinositol- and
phosphatidylcholine - transfer activity of
PITPbeta is essential for
COPI - mediated retrograde transport from the Golgi to the endoplasmic reticulum . -
pubmed
Phosphatidylinositol transfer protein beta (
PITPbeta ) , an essential protein that possesses
phosphatidylinositol (
PtdIns ) and
phosphatidylcholine (
PtdCho ) lipid transfer activity is known to localise to the Golgi and
ER but its role in these membrane systems is not clear . -
pubmed
Depletion of
PITPbeta leads to a decrease in
PtdIns ( 4 ) P levels , compaction of the Golgi complex and protection from
brefeldin - A - mediated dispersal to the
ER . -
pubmed
This phenotype can be rescued by expression of wild - type
PITPbeta but not by mutants defective in docking , PtdIns transfer and
PtdCho transfer . -
pubmed
These data demonstrate that the
PtdIns and
PtdCho exchange activity of
PITPbeta is essential for
COPI - mediated retrograde transport from the Golgi to the
ER . -
pubmed
SOD-1 was directly associated with
parasitaemia , plasma
creatinine and
alanine amino - transaminase levels , while
TNF - alpha correlated only with the later enzyme . -
pubmed
OBJECTIVE : This investigation assessed the effect of serum
estradiol levels on outcomes of in - vitro fertilization and embryo transfer (
IVF ) cycles . -
pubmed
MATERIALS AND METHOD : This was a retrospective cohort study of 1123
IVF cycles comparing impact of
estradiol ( E(2 ) ) levels on follicular development , fertilization , embryo quality , implantation , pregnancy rate ,
miscarriage rate , and selected obstetric complications . -
pubmed
[
Behcet ' s disease and associations with
HLA - B5 antigen ( a review of literature and the authors ' findings ) ] . -
pubmed
AIM : To estimate the distribution of
HLA Class I ( A , B )
antigens in patients with
Behcet ' s disease (
BD ) and the association of
HLA - B5 antigen with the clinical manifestations of the disease in different ethnic and population groups in relation to gender . -
pubmed
RESULTS : In patients with
BD , the prevalence of
HLA - B5 antigen proved to be significantly higher than that in the controls ( 72.0 and 21 % , respectively ) and to be similar in patients of different ethnicities living in the Caucasus and Transcaucasus ( 80 - 83 % ) while the number of
HLA - B5 antigen - positive patients with
BD was thrice less in the Russian population than in other
BD patients ( p & lt ; 0.01 ) . -
pubmed
This embodiment is superior to current
SLN mapping agents such as nontargeted
[ ( 99m ) Tc ] sulfur colloids and
Isosulfan Blue , as well as the phase III targeted ligand
[ ( 99m ) Tc]SPECT Lymphoseek counterpart , species that are visible by SPECT or visible absorbance separately . -
pubmed
Facile incorporation of
( 18 ) F into a
NIRF probe should promote many synergistic PET and NIRF combinations . -
pubmed
In
migraineurs ,
GTN administration was associated to a significant facilitation in temporal summation of
pain ( reduced
TST and increased
painful sensation ) 60 ' , 120 ' and 180 ' after drug intake when compared to baseline , to placebo condition and to controls after
GTN intake . -
pubmed
The results indicated that
caulophine and
taspine were the target component acting on the
epidermal growth factor receptor . -
pubmed
In the last decade , PET /
CT using
radiolabeled fluorodeoxyglucose has been added to the imaging armamentarium , mostly for imaging
lung cancer . -
pubmed
Phosphorylation of a
threonine residue (
Thr(11 ) ) in
Cds1 by
Rad3 occurs at a basal level in the absence of three other parallel
Rad3 - dependent phosphorylations on the mediator
Mrc1 and
Rad9 in the checkpoint
clamp complex . -
pubmed
However , the three parallel
Rad3 - dependent phosphorylations are all required for efficient phosphorylation of
Thr(11 ) in
Cds1 by
Rad3 . -
pubmed
Phosphorylation of
Thr(11 ) has been shown previously to promote autophosphorylation of
Thr(328 ) in the kinase domain of
Cds1 , which directly activates the enzyme , leading to full activation of the checkpoint pathway . -
pubmed
Through 2 - D gel electrophoresis and MALDI -
TOF -
TOF - based proteomics approaches , we found that
14 - 3 - 3beta , which was one of the proteins that were differentially expressed by
5 - fluorouracil - treated
gastric cancer SC -
M1 cells , was upregulated in
gastric cancer cells . -
pubmed
MTT score was higher in the
albumin group than in the control group ( P<0.05 ) . -
pubmed
As compared with that of the
albumin group ,
MTT score and
FN protein concentration decreased ,
FN mRNA significantly down - regulated in
NCTD intervention groups respectively ( P<0.05 ) . -
pubmed
Cd treatment induced a DNA hypermethylation , as well as an up - regulation of
CMT , indicating that de novo methylation did indeed occur . -
pubmed
The data demonstrate that
Cd perturbs the DNA methylation status through the involvement of a specific
methyltransferase . -
pubmed
FINDINGS : We show that inhibition of
Akt by the
Akt inhibitor X enhanced the production of
PGE2 and
PGD2 without affecting the expression of
COX-2 ,
mPGES-1 ,
mPGES-2 and
cytosolic prostaglandin E synthase (
cPGES ) . -
pubmed
In contrast , the
mTOR inhibitor
rapamycin enhanced both
COX-2 and
mPGES-1 immunoreactivity and the release of
PGE2 and
PGD2 . -
pubmed
Interestingly ,
NVP - BEZ235 , a dual
PI3K /
mTOR inhibitor , enhanced
COX-2 and reduced
mPGES-1 immunoreactivity , albeit
PGE2 and
PGD2 levels were enhanced in LPS - stimulated microglia . -
pubmed
CONCLUSION : Taken together , we demonstrate that blockade of
mTOR and/or
PI3K /
Akt enhances
prostanoid production and that
PI3K /
Akt ,
GSK-3 and
mTOR differently regulate the expression of
mPGES-1 and
COX-2 in activated primary microglia . -
pubmed
For preventing the
scar contracture of host tissue and adjusting the tensile strength of covering cell sheets , a controlled
collagen crosslinking step process in the preparation of skin - fibroblast sheets for repairing
wound was investigated by using
beta - aminopropionitrile (
BAPN ) , a
collagen crosslinking inhibitor , in the culture medium . -
pubmed
Collagen fibres of control cell sheets were more dense and thick than those of
BAPN - treated cell sheets , where the crosslinking of
collagen fibres was clearly inhibited . -
pubmed
Sodium dodecyl sulphate -
polyacrylamide gel electrophoresis (
SDS - PAGE ) showed that
BAPN - treated cell sheets gave no beta - chain band , indicating that the
collagen crosslinkings of the fibroblast sheets were able to be controlled by
BAPN . -
pubmed
Serum levels of
lactate dehydrogenase , blood
urea nitrogen , and
creatinine were elevated . -
pubmed
However , the amounts of
ZO-1 mRNA and protein in the corneal epithelium were not affected by
BAC treatment . -
pubmed
In fact , if the respiratory chain were present in the myelin membrane , then the
ATP synthase would function in reverse , hydrolyzing rather than synthesizing
ATP . -
pubmed
Polyphenols with a
catechin configuration were better
DHFR inhibitors than those with an
epicatechin configuration . -
pubmed
Derivatives with a
hydroxyl group para on the
ester - bonded
gallate moiety presented a high in vitro binding to
DHFR , but exhibited transport problems in cell culture due to ionization at physiologic pHs . -
pubmed
The impact of the binding of
catechins to
serum albumin on their biological activity was also evaluated . -
pubmed
The data suggest that changes in their structure to avoid
serum albumin interactions and to facilitate plasmatic membrane transport are essential for the intracellular functions of
catechins . -
pubmed
The study investigated whether successful transfer of game - based cognitive improvements to untrained tasks might be modulated by preexisting neuro - developmental factors , such as genetic variability related to the
catechol - O - methyltransferase (
COMT)-an enzyme responsible for the degradation of
dopamine . -
pubmed
We trained participants , genotyped for the
COMT Val(158)
Met polymorphism on playing " Half - Life 2 " , a first - person shooter game which has been shown to improve cognitive flexibility . -
pubmed
In addition , the applicability of electrochemical oxidation as an advanced
post - treatment for the complete removal of chemical
oxygen demand ( COD ) from the anaerobically treated cheese whey was evaluated . -
pubmed
When the anaerobic reactor effluent was
post - treated over a
boron - doped
diamond ( BDD ) anode at 9 and 18 A and in the presence of
NaCl as the supporting
electrolyte , complete removal of COD was attained after 3 - 4 h of reaction . -
pubmed
In the present study , a new kind of electroneutralized triblock copolymer ,
LPL , dendron G2(
L - lysine - Boc )- PEG2k - dendron G2(
L - lysine - Boc ) , was designed and investigated . -
pubmed
To evaluate knee intraarticular adhesion , we performed macroscopic evaluation , histological and
collagen density analyses ,
hydroxyproline content determination , fibroblast counting and densitometric analyses . -
pubmed
The results showed that loose
collagen tissues with little or no adhesion were present around the decorticated areas in the group treated with 0.5 mg / ml
colchicine . -
pubmed
Moreover ,
ACF decreased
Bcl-2 expression and increased
Bax expression . -
pubmed
Conversely , the ovarian zone exhibited a stable increase pattern which was similar to the plasma
17beta - estradiol (
E2 ) levels . -
pubmed
Through focused screening of our compound collection , we identified a series of
N - sulfonylated benzoxazepines , which displayed micromolar affinity for the
RORgamma ligand - binding domain ( LBD ) in a radioligand binding assay . -
pubmed
A cocrystal structure of inverse agonist 15 (
2 - chloro-6 - fluoro - N -( 4 -{[ 3 -( trifluoromethyl ) phenyl ] sulfonyl }- 2,3,4,5 - tetrahydro-1,4 - benzoxazepin-7 - yl ) benzamide ) bound to the
RORgamma - LBD illustrated that both hydrophobic interactions , leading to an induced fit around the substituted
benzamide moiety of 15 , as well as a
hydrogen bond from the
amide NH to
His479 seemed to be important for the mechanism of action . -
pubmed
This structure is compared with the structure of agonist 25 (
N -( 2 - fluorophenyl )- 4 -[( 4 - fluorophenyl ) sulfonyl ]- 2,3,4,5 - tetrahydro-1,4 - benzoxazepin-6 - amine ) and structures of other known
RORgamma modulators . -
pubmed
Emerging evidence suggest that upregulation of
arginase is of central importance for reduced
NO bioavailability due to competition for the substrate
L - arginine between
arginase and the endothelial form of
NO synthase . -
pubmed
Arginase is also associated with increased oxidative stress , further impairing
NO bioavailability . -
pubmed
RESULTS : The serum
glucose concentration decreased , the
insulin resistance and
insulin sensitivity significantly increased in treated groups . -
pubmed
CONCLUSIONS : Swimming exercises accompanied by consuming UD aqueous extracts effectively improved diabetic parameters , repaired pancreatic tissues in
streptozotocin - induced
diabetics in vivo , and increased
glucose uptake or
insulin in UD - treated cells in vitro . -
pubmed
There was no statistically significant difference in
hemoglobin ,
iron ,
hepcidin , and
sTfR concentration between the two studied groups . -
pubmed
There have been reports of successful treatment combining
doxycycline 100 mg and
montelukast 10 mg , dosed
BID for 3 months . -
pubmed
Recent randomized trials demonstrate reduced ischemic events including
myocardial infarction (
MI ) ,
stroke , and
death with continued
DAPT for up to 30 months or longer , particularly in the
post -
MI population . -
pubmed
Among isolates phenotypically identified as MSSA by antimicrobial susceptibility assays , we verified 30
oxacillin - susceptible isolates , which exhibited the
mecA gene , without mec complex amplification and were thus classified as OS - MRSA . -
pubmed
OBJECTIVES :
Celecoxib is a
cyclooxygenase-2 - specific inhibitor indicated to treat acute
pain and
pain secondary to
osteoarthritis and
rheumatoid arthritis . -
pubmed
The occurrence , which is dependent on the presence of
epidermal growth factor , of new Raman features associated with the appearance of
phosphothreonine and
phosphoserine residues reflects a signal transduction from the membrane to the nucleus , with concomitant modification of DNA / RNA structural characteristics . -
pubmed
The community composition and abundance of aerobic / anaerobic
ammonia /
ammonium - oxidizing microorganisms (
AOM ) in the aquitard were investigated in this study . -
pubmed
The
alpha subunit of ammonia monooxygenase gene (
amoA ) was used as the biomarker for the detection of aerobic
ammonia - oxidizing archaea ( AOA ) and bacteria ( AOB ) , and also partial
16S rRNA gene for Plantomycetes and anaerobic
ammonium - oxidizing ( anammox ) bacteria . -
pubmed
The Pearson moment correlation analysis showed that
AOA amoA gene abundance positively correlated with pH and
ammonium concentration , whereas
AOB amoA gene abundance negatively correlated with C / N ratio . -
pubmed
This is the first report that highlights the presence with low diversity of
AOM communities in natural aquitard of rich
ammonium . -
pubmed
Pre and postmeal samples were analysed for total
bile acids ,
parathyroid hormone and C - terminal telopeptide . -
pubmed
Results Body
weight loss post Roux - en - Y gastric bypass was associated with a median 4.9 - fold increase in peak postprandial total
bile acid concentration , and a median 2.4 - fold increase in cumulative food evoked
bile acid response . -
pubmed
These results suggest that
post - Roux - en - Y gastric bypass increases in total
bile acids do not effectively oppose an ongoing resorptive signal operative along the gut - bone axis . -
pubmed
Intracoronary
adenosine - induced
ventricular arrhythmias during fractional flow reserve (
FFR ) measurement : case series and literature review . -
pubmed
It is based on the change in the pressure gradient across the
stenosis after the achievement of maximal
hyperemia of the coronary circulation which is commonly induced by intravenous (
IV ) or intracoronary ( IC ) administration of
adenosine . -
pubmed
Here , we have described three cases of IC
adenosine - induced
ventricular arrhythmias during
FFR measurement from our institution , and after literature review we found that all the cases of
ventricular arrhythmias induced by
adenosine during
FFR measurement were observed where it was administered via IC route . -
pubmed
Although a causal relationship between the use of IC
adenosine during
FFR measurement and the induction of
ventricular arrhythmias is not yet established , we suggest using
IV adenosine as the preferred route of administration until we better understand the incidence and mechanism underlying this phenomenon . -
pubmed
We showed that the migration of hDPSCs was induced by
SDF-1 in a concentration - dependent manner and could be inhibited with siCXCR4 (
C - X - C chemokine receptor type 4 ) and
siCDC42 (
cell division control protein 42 ) , as well as drug inhibitors such as
AMD3100 ( antagonist of
CXCR4 ) ,
LY294002 ( inhibitor of
PI3 K ) and
PF573228 ( inhibitor of
FAK ) . -
pubmed
OBJECTIVE : To compare subjective , objective and safety performance of
silicone hydrogel ( SiHy ) daily disposable (
DD ) with hydrogel ( Hy ) DD contact lenses . -
pubmed
Reversible
janus associated kinase (
JAK ) inhibitors such as
tofacitinib and
decernotinib block
cytokine signaling and are efficacious in treating
autoimmune diseases . -
pubmed
Verapamil , a
P - glycoprotein inhibitor , significantly increased the absorption of
4,8 - sphingadienine but did not affect the absorption of
sphingosine . -
pubmed
They both negatively regulate
OprD porin expression which affects the intake of antibiotics like
carbapenems . -
pubmed
More specifically ,
Lico B induced apoptosis through the regulation of
specificity protein 1 and apoptosis - related proteins including
CCAAT / enhancer - binding protein homologous protein ,
death receptors , and
poly ( ADP - ribose ) polymerase . -
pubmed
In vitro , primary chondrocytes were treated with
corticosterone ( 0 - 1250 nM ) ,
caffeine ( 0 - 100 muM ) ,
mitogen - inducible gene 6 (
Mig-6 ) siRNA and
epidermal growth factor receptor (
EGFR ) siRNA , respectively , or together . -
pubmed
Furthermore ,
PCE upregulated
Mig-6 and suppressed
EGFR expression in the
HZ . -
pubmed
In vitro , a high - concentration
corticosterone ( 1250 nM ) upregulated
Mig-6 expression , inhibit
EGFR /
c - Jun N - terminal kinase (
JNK ) signaling pathway and terminal differentiation genes expression in chondrocytes and reduced cell apoptosis , and these above alterations could be partly reversed step - by - step after
Mig-6 and
EGFR knockdown . -
pubmed
Ipragliflozin treatment resulted in statistically significant improvements versus baseline in
hemoglobin A1c , fasting plasma
glucose concentration ,
body weight , blood pressure , heart rate , and serum concentrations of
low - density lipoprotein cholesterol and
triglycerides . -
pubmed
One
pot biocatalytic synthesis of a biodegradable electroactive macromonomer based on
3,4 - ethylenedioxytiophene and
poly ( l - lactic acid ) . -
pubmed
All macromonomers (
EDOT - PLLA ) were characterized by
1H and
13C RMN , MALDI -
TOF , GPC and EDX . -
pubmed
However , in the case of established persistent
viral infection , sustained elevation of
IFN - I expression bears deleterious effects to the host and is
today considered as the major driver of
inflammation and immunosuppression . -
pubmed
INTRODUCTION :
Ertugliflozin is an oral
sodium - glucose cotransporter 2 inhibitor that is being developed to treat
type 2 diabetes mellitus (
T2DM ) . -
pubmed
At week 26 , both
ertugliflozin /
sitagliptin treatments provided significant reductions from baseline in HbA1c compared with placebo [ least squares mean HbA1c change ( 95 % confidence intervals ) from baseline was - 0.4 % ( - 0.7 , - 0.2 ) , - 1.6 % ( - 1.8 , - 1.4 ) , and - 1.7 % ( - 1.9 , - 1.5 ) for placebo ,
E5 /
S100 , and E15 /
S100 , respectively ] . -
pubmed
Significant reductions in fasting plasma
glucose , 2 - h
post - prandial
glucose ,
body weight , and
systolic blood pressure were observed with both
ertugliflozin /
sitagliptin groups compared with placebo . -
pubmed
Regarding the cell attachment , cells cultured on BaGs had enhanced
integrinbeta1 and
vinculin production , and mature focal adhesions were smaller but more dispersed than on cell culture plastic (
polystyrene ) . -
pubmed
Moreover , the BaG - stimulated osteoinduction was significantly reduced by
FAK and
mitogen - activated protein kinase (
MAPK ) inhibitors , indicating an important role for
FAK and
MAPKs in the
BaG - induced early osteogenic commitment of hASCs . -
pubmed
Discovery of a novel
2 -( 1H - pyrazolo [ 3,4 - b ] pyridin-1 - yl ) thiazole derivative as an
EP1 receptor antagonist and in vivo studies in a
bone fracture model . -
pubmed
A high - throughput immunoassay technique that quantitates the expression of relevant proteins of glycolysis , glycogenolysis ,
pentose phosphate pathway , oxidative phosphorylation ,
pyruvate and
fatty acid oxidation ,
tricarboxylic acid cycle and the antioxidant response in two large independent and retrospectively collected cohorts of
PEO - sD ,
PEO - mD and
MELAS patients revealed that despite the heterogeneity of the genetic alterations , the three MDs showed the same metabolic signatures in both cohorts of patients , which were highly divergent from those of healthy individuals . -
pubmed
Finally , we found
TCL1 to act , at least in part , via increasing
phospho -
ERK1 / 2 and decreasing
phospho -
P38 MAPK . -
pubmed
Because the
PPARgamma antagonist
BADGE and
PI3K /
Akt inhibitors
wortmannin and
LY29004 inhibited the intracellular lipid accumulation by NA and
NAG associated with adipogenesis , it was considered that NA and
NAG showed the above - mentioned activities via the activation of
PPARgamma as well as phosphorylation of the
PI3K /
Akt pathway . -
pubmed
Plants subjected to
Pb - stress showed various deteriorations in cell organelles including visible alterations in
chloroplasts , malformations in plant cells , abnormalities in the mitochondrial system , inward invagination of cell walls , distortions in the plasma membrane , oversized vacuoles and irregular increase in plastoglobuli formation . -
pubmed
The mRNA levels of
IFN - gamma was examined in ( n = 23 ) polyomavirus BK infected and ( n = 23 ) non - infected kidney transplant patients in comparison with healthy controls ( n = 23 ) , using an in - house Real time PCR (
SYBR Green ) assay . -
pubmed
NADPH oxidases are enzymes that have been reported to generate reactive
oxygen species ( ROS ) in animals , plants and many multicellular fungi in response to environmental stresses . -
pubmed
Additionally , the transcription of
vvnoxa ,
vvrac1 ,
vvbema and
vvcdc24 could be repressed when the intracellular ROS were eliminated by
diphenyleneiodonium (
DPI )
chloride and
reduced glutathione (
GSH ) treatments . -
pubmed
METHODS : In vitro 2 - D and 3 - D assays , and intracellular
calcium influx assays were utilized to measure tumourigenic activity in response to altered
CANCG4 levels and
calcium channel blockers . -
pubmed
CACNG4 functions by closing the channel pore , inhibiting
calcium influx , and altering
calcium signaling events involving key survival and metastatic pathway genes (
AKT2 ,
HDAC3 ,
RASA1 and
PKCzeta ) . -
pubmed
Our findings suggest an underlying pathway for
tumour growth and dissemination regulated by
CACNG4 that is significant with respect to developing treatments that target these channels in
tumours with aberrant
calcium signaling . -
pubmed
Glioblastoma Multiforme Stem Cell Cycle Arrest by
Alkylaminophenol Through the Modulation of
EGFR and CSC Signaling Pathways . -
pubmed
THTMP strongly inhibited
NSCC and in a subtle way for
GSC in a time - dependent manner and inhibit the resistance variants better than that of
temozolomide (
TMZ ) . -
pubmed
THTMP arrest the CSC cell population at both
G1 / S and
G2 / M phase and induce ROS - mediated apoptosis . -
pubmed
THTMP arrests
GBM stem cell cycle through the modulation of
EGFR and CSC signaling pathways . -
pubmed
The coding sequences of
lcTRAF2 ,
lcTRAF7 , and
lcTRADD were 1488 , 2454 , and 744 nucleotides , and they encoded proteins of 495 , 344 , and 248
amino acids , respectively . -
pubmed
The aim of the study was to make a comparative studies on the effectiveness of platelet rich plasma (
PRP ) and
hyaluronic acid ( HA ) in intra - articular injections to the temporomandibular joints - in double blind studies application - based on the analysis of selected clinical parameters of functional efficiency and the mean value of joint 's
pain intensity before and after management . -
pubmed
Study group
PRP was treated with intra - articular injection of platelet rich plasma and study group HA had injection with
hyaluronic acid . -
pubmed
Carboxylated nanocrystalline cellulose ( CNCC ) was prepared by oxidation degradation of
microcrystalline cellulose (
MCC ) using
ammonium peroxydisulfate and modified with
Fe3O4 nanoparticles to form
Fe3O4 - CNCC nanocomposite via simple refluxing process . -
pubmed
The
Fe3O4 - CNCC nanocomposite doped
poly ( 3,4 - ethylenedioxythiophene ) (
PEDOT ) was successfully decorated on the
glassy carbon electrode (
GCE ) by electrochemical deposition . -
pubmed
The
PEDOT /
Fe3O4 - CNCC modified
GCE with enlarged real electrochemical surface area was used to determine
nitrite with high selectivity , sensitivity and outstanding reproducibility . -
pubmed
Here , we synthesized gold nanoparticles coated with selected osmolytes (
proline ,
hydroxyproline , and
glycine ) and examined their effect on temperature - induced amyloid - formation of
insulin hormone . -
pubmed
METHODS :
Jagged ends of cfDNA were detected by incorporating unmethylated
cytosines during a DNA end - repair process , followed by
bisulfite sequencing . -
pubmed
Previously , we showed that in vitro
morphine treatment significantly elevates
Cav-1 expression and causes neuroplasticity changes . -
pubmed
In this study , we confirmed that chronic
morphine exposure can significantly increase
Cav-1 expression ( P < 0.05 ) and
microtubule - associated protein (
MAP-2)-positive neuronal dendritic growth in the hippocampus . -
pubmed
Moreover , the rewarding effect and dendritic growth in the HPC induced by chronic
morphine exposure were significantly inhibited by hippocampal
Cav-1 knockdown . -
pubmed
Together , these data suggest that
Cav-1 in the hippocampus plays an essential role in the neuroplasticity changes that underlie
morphine addiction behaviors . -
pubmed
We have investigated the effects of the drugs
cytochalasin B and
colchicine on the surface levels of the
large , external , transformation - sensitive ( LETS ) glycoprotein . -
pubmed
Colchicine neither removed
LETS protein from the surface , nor inhibited its regeneration after removal by mild trypsinization . -
pubmed
Cells treated with
cytochalasin B , however , showed both a 2 - 3 - fold increase in the turnover rate of their surface
LETS protein and a marked inhibition in its regeneration . -
pubmed
In fact , in the presence of
cytochalasin B , increased quantities of
LETS protein were released into the medium . -
pubmed
In consideration of the patient 's age a conservative therapeutic program was instituted with a definite response to I-131 therapy despite poor radioactive
iodine (
RAI ) uptake by the
tumor . -
pubmed
Partial nucleotide sequence analysis of the cDNA revealed a deletion of 243 nucleotides coding for 81
amino acids in a portion of the
immunoglobulin - like domains of the extracellular region of the receptor . -
pubmed
Do analogues of
gonadotrophin releasing hormone influence follicular fluid
steroid levels , oocyte maturity and fertilization rates ? -
pubmed
The inflammatory effects of
enalaprilat and
cilazaprilat were tested in an experimental model of
ovalbumin - sensitised guinea - pigs . -
pubmed
Enalaprilat , but not
cilazaprilat , enhanced the
ovalbumin - induced inflammatory skin responses . -
pubmed
Enalaprilat , applied locally , but not
cilazaprilat , enhanced the inflammatory reactions caused by intradermal injections of allergen and
substance P. -
pubmed
Our study strongly indicates that
enalaprilat has pro - inflammatory properties , whereas the new long - acting
ACE inhibitor
cilazaprilat does not . -
pubmed
An objective of the present experiment was to measure the
post -
hypoxic drinking response at intervals during a 71 - d exposure to an atmosphere containing 12 %
oxygen in
nitrogen to assess whether the response could be observed throughout this entire period . -
pubmed
However , the results of these studies indicate that administration of the
betra - adrenoceptor antagonist ,
propranolol ( 6 mg / kg , i.p . ) -
pubmed
These patients showed a more severe
thalassaemia intermedia like clinical phenotype as compared to those with the same
beta - globin genotype and a normal alpha -
globin gene arrangement . -
pubmed
A group of patients with
thalassaemia intermedia from Southern Italy , either homozygous or heterozygous for haplotype I and in whom previous studies had failed to define the mutation in one of the
beta thalassaemia globin genes , were screened by oligonucleotide analysis for the presence of the
beta th-101 . -
pubmed
Chlordecone has been reported to resemble
estrogen in altering pituitary secretions , in producing vaginal cornification , in increasing uterine weight and in competing for binding to the
estrogen receptor . -
pubmed
In further studies ,
chlordecone 's effect on the CNS
progesterone receptor was examined . -
pubmed
Unlike
estradiol ,
chlordecone did not induce
progesterone receptors . -
pubmed
Furthermore ,
chlordecone attenuated the increase in
progesterone receptors seen after
estradiol treatment . -
pubmed
Pressure - volume curves were performed prior to and following the initiation of artificial pulmonary perfusion with 1 %
bovine serum albumin and 5 %
dextran in Tyrode 's
solution . -
pubmed
Morphine ( an agent inhibiting
substance P release ) significantly attenuated the
spasm , whereas the presence of
substance P in the perfusate markedly enhanced the constriction . -
pubmed
Depletion of endogenous
substance P by chronic
capsaicin pretreatment did not affect exogenous
substance P - induced
spasm . -
pubmed
Effect of
zinc ions on
tRNA structure . -
pubmed
RPC-5 contains
dichlorodifluoroethylene beads coated with a
quaternary ammonium compound where the substituents are :
R1 =
methyl , and
R2 - 4 =
C8 - 10 hydrocarbons . -
pubmed
Aminex A-28 contains
quaternary ammonium covalently attached to
styrene -
divinylbenzene copolymer lattice and
R1 - 3 are
methyl groups . -
pubmed
The chromatography of
tRNA in the presence and absence of
Zn2 + is interpreted in terms of this model and the effects of
sodium chloride concentration , temperature , and pH were explored as the experimental variables . -
pubmed
These experiments suggest that in the absence of
Zn2 + tRNA does not interact appreciably with the hydrophobic surface of the column . -
pubmed
The addition of
Zn2 + has three effects on chromatography : a decrease in the number of anionic sites on the
tRNA which interact with the positively charged
ammonium ion , an increase in affinity of the
tRNA for these ionic sites , and an increase in affinity of
tRNA for hydrophobic sites on the column . -
pubmed
These results show that chromatography on
RCP-5 can be a sensitive physical chemical technique for examination of the structure of
tRNA , and probably for other nucleic acids as well . -
pubmed
The
tyrosine hydroxylase inhibitor
alpha - methyltyrosine almost completely inhibited the accumulation of
DOPA in all organs . -
pubmed
The
DOPA accumulation was enhanced in all organs by the
alpha - adrenoceptor antagonists
phentolamine and
yohimbine . -
pubmed
These results indicate ( a ) that sporozoites and merozoites share
antigens recognized by serum
IgG , ( b ) that there is no episode of marked immunosuppression during
acute infection , and ( c ) that cellular immunity is probably more important in resistance against reinfection than humoral immunity . -
pubmed
Such bronchoalveolar mast cells will release
histamine in response to
IgE - dependent challenge in a reaction that is dose- , time- and energy - dependent . -
pubmed
In addition , bronchoalveolar mast cells from
asthmatic subjects show an increased releasability of
histamine in response to
anti - IgE. -
pubmed
Storage was evaluated by application of dual immunocytochemical stains with anti -
LH beta and anti -
FSH beta to cells stimulated with 0.5 nM
[ D - Lys6 ] GnRH . -
pubmed
GnRH had less striking effects on changing the size or number of
LH and
FSH plaques . -
pubmed
In the basal state , there were 36.6 + /- 9 %
LH and only 2.7 + /- 2 %
FSH plaques , whereas after
GnRH stimulation there were 43.1 + /- 10 %
LH and 10.5 + /- 4 %
FSH plaques . -
pubmed
Tissue was double labeled for [
3H]
GABA uptake and
glutamate decarboxylase (
GAD ) immunolocalization . -
pubmed
Nearly 90 % of the amacrine cell synaptic processes onto both proximal and distal halves of mb1 terminals were labeled with either [
3H]
GABA or
GAD - immunoreactivity ( IR ) . -
pubmed
Proximal half : 73 % of the amacrine synapses were labeled with [
3H]
GABA uptake and 82 % with
GAD - IR ; 88 % of [
3H]
GABA labeled contacts were double labeled . -
pubmed
Distal half : 17 % of the amacrine synapses were labeled with [
3H]
GABA uptake and 67 % with
GAD - IR ; 63 % of [
3H]
GABA labeled contacts were double labeled . -
pubmed
[ The
opiate sigma - agonist
SKF 10.047 disturbs the early morphogenesis of the loach ] . -
pubmed
The immunogen and
enzyme marker were prepared by direct reaction of
secalonic acid D with
bovine serum albumin and
horseradish peroxidase , respectively . -
pubmed
[ Variations in the induced optic activity accompanying the association of certain
dicarboxylic acids to
aspartate aminotransferase ] . -
pubmed
Four had positive findings simultaneously for
hepatitis B surface antigen (
HBsAg ) and
surface antibody , and five had positive findings for
HBsAg alone . -
pubmed
Certain cyclic compounds with a
butyrolactone ,
tetrahydrofuran ,
furan ,
tetrahydropyran , or
2 - pyron ring showed substantial rescue activity in
lon and ( or )
ruv mutants . -
pubmed
The data imply that intracollicular mechanisms activated by
NMDA and non -
NMDA receptors contribute to the generation of the inhibitory responses in SCC which lead to response habituation . -
pubmed
The homology to AAV2 was also confirmed at the
amino acid level (
nonstructural protein 55 % , capsid coding region 51 % ) . -
pubmed
Northern analysis indicates that a 2.7 - kb
unc-7 message is present in all developmental stages but is most abundant in
L1 -
L3 larvae . -
pubmed
We determined the sequence of a cDNA corresponding to the
unc-7 message . -
pubmed
The
message may encode a 60 - kd protein whose
amino acid sequence is unrelated to any other available protein sequence ; a transmembrane location for the
unc-7 protein is predicted . -
pubmed
We speculate that
unc-7 may be involved in the function of neuronal
ion channels . -
pubmed
The circumvention of
P - glycoprotein function by pharmacologic agents is a major focus of clinical trials aimed at increasing the
cytotoxicity of multidrug resistance - associated drugs , including
doxorubicin . -
pubmed
UTP ,
alpha , beta - Me - ATP and
ATP ( in the presence of an
A1 purinoceptor antagonist ) produced a depolarisation in that order of potency . -
pubmed
Of these , 32
tRNA species were identified by aminoacylation as
tRNA specific for 15
amino acids . -
pubmed
3 - Aminobenzamide (
3 - ABA ) , a specific inhibitor of
poly ( ADP - ribosyl ) polymerase (
PARP ) , a nuclear enzyme which is activated by breaks in DNA to catabolize intracellular
NAD , is capable of relieving such a drop . -
pubmed
At 20 h , when the apoptotic ladder - like pattern of DNA is visible ,
NAD concentration has dropped again , probably because of a second
PARP activation due to the extensive DNA degradation that accompanies apoptosis . -
pubmed
The presence of
3 - ABA , concomitantly with the preservation of the intracellular
NAD content , reduces the extent of apoptosis upon oxidative stress and strongly enhances cell survival , thus suggesting a role for
PARP in triggering stress - induced apoptosis . -
pubmed
Moreover , in these instances the inhibition of
PARP , although effective in blocking the drop in
NAD concentration , has no effect on apoptosis , thus being only circumstantial . -
pubmed
The LPSR has a comparatively high threshold of activation , its rate of development is inversely related to
magnesium concentration and it can be eliminated by
NMDA antagonists , as can the induction of
PP . -
pubmed
Quinazoline antifolates inhibiting
thymidylate synthase : synthesis of gamma - linked peptide and
amide analogues of
2 - desamino-2 - methyl - N10 - propargyl - 5,8 - dideazafolic acid (
ICI 198583 ) . -
pubmed
Sensitive northern blot hybridization using
digoxigenin RNA probes for the mRNA detection of two
glucose transporter isoforms . -
pubmed
This work shows that
DIG - labelled
glucose transporter (
GLUT1 and
GLUT4 ) cRNAs are suitable probes for the assessment of these gene expressions . -
pubmed
We have found that the
DIG system offers a much higher sensitivity than the
32P system for both
GLUT1 and
GLUT4 mRNA detection . -
pubmed
In addition , stability , safety , time saving and cost reduction are other considerations that make
DIG - labelled
GLUT1 and
GLUT4 cRNAs very attractive . -
pubmed
We have solved the 2.0 - A resolution crystal structures of four cavity - creating
Ile /
Leu-->
Ala mutations in the hydrophobic core of
barnase and compare and contrast the structural responses to mutation with those found for
Leu-->
Ala mutations in
T4 lysozyme . -
pubmed
Second , although it is currently accepted that hydrophobic cavities formed on the mutation of large hydrophobic
side chains to smaller ones are not occupied by
water molecules , we found a buried
water molecule in the crystal structure of the
barnase mutant Ile76 - ->
Ala . -
pubmed
Notably , the
hydrogen bond between the cavity - water and the
carbonyl group of
Phe7 is also a marked early feature of very recent molecular dynamics simulations of
barnase denaturation [ Caflisch , A. , & amp ; Karplus , M. ( 1995 ) J. Mol . -
pubmed
It is possible that cavities engineered into the hydrophobic cores of other
proteins may contain
water molecules , even though they can not be detected by crystallographic analysis . -
pubmed
Using the monoclonal
MIB-1 antibody , which detects
Ki-67 antigen in
formalin - fixed , paraffin - embedded tissue , immunohistochemical studies were performed . -
pubmed
Luteal phase
progesterone profiles were assessed between the days
LH surge +2 and
LH surge +6 by means of a
progesterone index . -
pubmed
Similarly , no relation was established between
LH and the quantitative assessment of luteal phase
progesterone profiles (
progesterone index ) , and the distribution of
progesterone indices was similar in the normal
LH and elevated
LH groups . -
pubmed
Testosterone concentrations were positively correlated with
LH ( p = 0.008 ) but not with the incidence of spontaneous follicular growth . -
pubmed
CONCLUSION : The data indicate that both
LH and
testosterone secretion in
PCOS were closely linked , but that neither was directly linked to the incidence or inhibition of spontaneous follicular development in
PCOS , or to the disturbance in luteal phase
progesterone profiles . -
pubmed
In particular , the
aromatase - immunoreactive cells of the lateral POM appear to be a key target for
steroids in the activation of male copulatory behavior . -
pubmed
The perfusion medium consisted of 95 %
O2 - 5 %
CO2 Krebs - Henseleit buffer , pH 7.4 , containing 5 g/100 ml
bovine serum albumin , 100 microU / ml
insulin , 5 mmol / l
glucose ,
amino acids , and added
fatty acids (
FA ) , including 0.1 microCi / ml
[ 3H ] palmitate . -
pubmed
Thus the relatively high
TG synthesis rates observed in the high - oxidative muscle sections are not uniquely related to their high - flow capacities but are inherent to the
TG synthesis process , probably events associated with
FA uptake and/or capacity of the
TG synthesis pathway . -
pubmed
Similarly ,
InsP6 ( 100 microM , 30 s ) had no effect on basal
superoxide anion generation and , compared to lipopolysaccharide ( LPS , 100 ng ml-1 , 60 min ) ,
tumour necrosis factor - alpha (
TNF alpha , 200 u ml-1 , 30 min ) or
platelet - activating factor (
PAF , 100 nM , 5 min ) caused only a small enhancement of 100 nM fMLP - stimulated
superoxide anion generation ( fold - increase in
superoxide anion generation over fMLP alone :
InsP6 1.8 + /- 0.3 , LPS 6.8 + /- 0.6 ,
TNF alpha 5.2 + /- 0.7 ,
PAF 5.8 + /- 0.6 ) . -
pubmed
The authors examined therefore the thyroid function (
thyroid stimulating hormone ,
thyroxine ,
triiodothyronine , antibodies against
thyroid peroxidase and against
thyroglobulin ) in a random sample of 76 women after ablation of the breast on account of
carcinoma . -
pubmed
With a coding sequence of 315 nucleotides ( nt ) , corresponding to a protein of 104
amino acids , the
SpliMNPV p10 gene is the longest
p10 gene known . -
pubmed
Upstream of the
p10 sequences , an ORF of 552 bp was identified that potentially encodes a 184
amino acid protein of Mr 20925 , which showed 52.2 % identity with the encoded product of the
SeMNPV xb187 gene . -
pubmed
Immunologic effects of
progesterone are mediated by a protein named the
progesterone - induced blocking factor (
PIBF ) . -
pubmed
The prolonged and excessive consumption of
alcohol has been shown to predispose the host to a variety of infectious complications , which may be due , in part , to the inability to produce important activating and chemotactic
cytokines . -
pubmed
These findings indicate that
ethanol feeding results in diminished production of chemotactic and/or activating
cytokines from AMs ex vivo that may contribute to the impairment in lung inflammatory responses and antimicrobial host defense that is observed in the setting of
alcohol ingestion / intoxication clinically and experimentally . -
pubmed
HTLV - II - infected subjects had on average , an 11 % decrease in
creatine kinase ( p = .006 ) , a minor increase in mean corpuscular volume ( p = .01 ) , and a slightly lower serum
calcium level ( p = .0005 ) . -
pubmed
It augmented
insulin release from islets incubated for 90 min in the absence or presence of
D - glucose ( 2.8 to 16.7 mM ) , this coinciding with stimulation of
45Ca net uptake . -
pubmed
In perifused islets deprived of extracellular
D - glucose for 45 min ,
BTS 67 582 ( 0.1 mM ) decreased 86Rb outflow from prelabelled islets , but failed to increase
45Ca efflux and
insulin release . -
pubmed
In the presence of
D - glucose ( 7.0 mM ) ,
BTS 67 582 , whilst failing to decrease
86Rb + outflow , provoked rapid , sustained and rapidly reversible increases of both
45Ca2 + efflux and
insulin output . -
pubmed
BTS 67 582 ( 0.1 mM ) suppressed the inhibitory action of
diazoxide ( 0.25 mM ) upon
glucose - stimulated
insulin release , but nevertheless augmented
insulin output from islets incubated in the presence of 90 mM
K + . -
pubmed
All five mutations cause high spontaneous
tyrosine phosphorylation of
KIT . -
pubmed
Instability of Taylor - Couette flow of
helium II . -
pubmed
The two receptors are
tyrosine kinases and have an extracellular domain containing seven
immunoglobulin - like loops and a split
tyrosine - kinase domain . -
pubmed
METHODS : A synthetic
tyrosine phosphopeptide was used to raise an antibody that recognizes the phosphorylation state of
tyrosine 1054/1059 in the
KDR receptor . -
pubmed
The IC50 values of
sufentanil binding with and without
fluvoxamine addition are 0.56+/-0.17 nM and 0.3+/-0.15 nM , respectively , indicating no direct effect on the occupancy of
sufentanil on the
mu - receptor by this
serotonin reuptake inhibitor . -
pubmed
HS - SPME coupled with
GC -
MS and HPLC techniques were employed to study the evolution of the volatile compounds and the individual
anthocyanins , respectively . -
pubmed
A method for the determination of underivatized
amino acids based on capillary electrophoresis coupled to electrospray ionization mass spectrometry ( CE - ESI -
MS ) is described . -
pubmed
Under the optimized conditions , 19 free
amino acids normally found in
proteins and several physiological
amino acids were well determined in less than 17 min . -
pubmed
OBJECTIVES : To investigate whether the same dipolar model could explain the scalp
CO ( 2 ) laser evoked potential (
LEP ) distribution after either hand or foot skin stimulation . -
pubmed
Phototoxicity was quantified at various periods after exposure by fluorescence staining of the nuclei of membrane - compromised cells , by TdT -
dUTP terminal nick - end labeling ( TUNEL ) of apoptotic cells and by
Annexin V labeling for
phosphatidylserine exposure . -
pubmed
Intrathecal pretreatment with
protein kinase C inhibitor
calphostin C ( 100 and 250 ng ) attenuated the decrease of the paw withdrawal latency evoked by sciatic nerve ligation . -
pubmed
The transport of
iodide across the cell membrane is mediated by the
sodium iodide symporter (
hNIS ) . -
pubmed
Therefore , the transduction of the
hNIS gene per se is sufficient to induce
125I (- ) transport in
Morris hepatoma cells in vitro and in vivo . -
pubmed
For a therapeutic application of the
hNIS gene , however , additional conditions need to be defined which inhibit the
iodide efflux . -
pubmed
PDMP and
phospholipase A2 inhibitors also blocked it . -
pubmed
However ,
clofibrate was distinguished from
BFA in that
BFA action was insensitive to
phospholipase A2 inhibitors and from
NDGA in that
NDGA stabilized microtubules against
nocodazole and its action was almost insensitive to
taxol . -
pubmed
GDF-15 is a potent trophic factor for developing and
6 - OHDA - lesioned midbrain dopaminergic neurons in vitro and in vivo , matching the potency of
GDNF . -
pubmed
The released
VEGF was 165
amino acid residues in either condition . -
pubmed
The enzyme activity was measured in a reaction mixture containing either vehicle or
calcium chloride ( 1 - 20 microM ) in the absence or presence of
regucalcin ( 0.1 , 0.25 , or 0.5 microM ) . -
pubmed
NO synthase activity was significantly increased by the addition of
calcium ( 5 - 20 microM ) . -
pubmed
This increase was completely abolished in the presence of
trifluoperazine (
TFP ; 10 - 50 microM ) , an antagonist of
Ca ( 2 +)/ calmodulin . -
pubmed
The addition of
regucalcin ( 0.1 - 0.5 microM ) caused a significant fall in the
calcium - increased enzyme activity . -
pubmed
The effect of
regucalcin ( 0.25 microM ) in decreasing
NO synthase activity was seen in the presence of
ethylene glycol bis -( 2 - aminoethylether ) N , N , N ' , N '- tetraacetic acid (
EGTA , 1 mM ) or
TFP ( 20 microM ) , indicating that
regucalcin acts independent on
Ca ( 2 +)/ calmodulin . -
pubmed
The high - loaded ( 48 - 60 wt.% ) 2 - 4 nm tetragonal
ZrO2 phase inserted in mesostructured
silica SBA-15 by chemical solution decomposition (
CSD ) of
Zr ( n - PrO ) 4 and activated at 873 K displayed approximately 3 times higher capacity for surface
sulfate ions and , respectively , 1.5 - 2.2 times higher catalytic activity per gram of
SO4 - ZrO2 /
SBA-15 composite in condensation of
MeOH with
t - BuOH and
dehydration of
isopropanol compared with the regular bulk sulfated
zirconia material . -
pubmed
This screen identified a catalytic triad (
Ser82 ,
His235 , and
Asp207 ) with a configuration similar to that of the catalytic
triad of
hydrolases . -
pubmed
Analysis of
BioH with a panel of
hydrolase assays revealed a
carboxylesterase activity with a preference for short
acyl chain substrates . -
pubmed
[ Comparative content of
amino acids in the
casein of the milk of certain animal species ] . -
pubmed
Its efficacy appears to be related to induction of
glutathione S - transferase enzymes , inhibition of
prostaglandin E ( 2 ) (
PGE ( 2 ) ) production , or suppression of oxidative DNA adduct ( M(1)G ) formation . -
pubmed
A daily oral dose of 3.6 g of
curcumin is advocated for Phase
II evaluation in the prevention or treatment of
cancers outside the gastrointestinal tract . -
pubmed
Additionally ,
serum LH and
progesterone concentrations were studied to investigate whether premature luteinization was present in these cycles and whether it might be related to coasting duration . -
pubmed
Effect of ionic strength on the organization and dynamics of
tryptophan residues in
erythroid spectrin : a fluorescence approach . -
pubmed
In this article , we have used a combination of fluorescence spectroscopic approaches that include red edge excitation shift ( REES ) , fluorescence quenching , time - resolved fluorescence measurements , and chemical modification of the
spectrin tryptophans to assess the environment and dynamics of
tryptophan residues of
spectrin under different ionic strength conditions . -
pubmed
Our results show that while REES , fluorescence anisotropy , lifetime , and chemical modification of
spectrin tryptophans remain unaltered in low and high ionic strength conditions , quenching of
tryptophan fluorescence by the aqueous quencher
acrylamide ( but not the hydrophobic quencher
trichloroethanol ) and resonance energy transfer to a
dansyl - labeled
fatty acid show differences in
tryptophan environment . -
pubmed
Surprisingly , however , a combination of
Phe-781 and
Gly-604 could restore about 44 % of the
SSIIa activity provided that
Val-737 was conserved . -
pubmed
Choline is formed as a result of the catalytic hydrolysis ( depending on the
cholinesterase activity ) of the substrate
acetylcholine . -
pubmed
tRNA - guanine transglycosylase (
TGT ) is a key enzyme involved in the
post - transcriptional modification of certain tRNAs in their anticodon wobble positions with
queuine . -
pubmed
To maintain the correct Watson - Crick base pairing properties of the wobble base ( and hence proper translation of the genetic code ) ,
TGT must recognize its
heterocyclic substrate with high specificity . -
pubmed
Herein , we report further studies probing the differential heterocyclic substrate recognition properties of the
aspartate 143 mutant
TGTs . -
pubmed
This confirms the key role of
aspartate 143 in maintaining the anticodon identities of the
queuine - containing tRNAs and suggests that
TGT mutants could be developed that would alter the
tRNA wobble base base pairing properties . -
pubmed
Photosynthetic assimilation of
carbon dioxide and
acetate by isolated
chloroplasts . -
pubmed
They seem to be involved in
aromatase expression , generation of
estrogens and activation of
estrogen receptor alpha (
ERalpha ) in
malignant breast epithelium . -
pubmed
Namely , it increased activation of
estrogen receptors , therefore , it was suspected to reduce or even overcome the inhibitory effect of
tamoxifen on breast cell proliferation . -
pubmed
Enzyme conformational dynamics during catalysis and in the ' resting state ' monitored by
hydrogen /
deuterium exchange mass spectrometry . -
pubmed
This work reports the use of electrospray mass spectrometry for studying the conformational dynamics of
enzymes by
amide hydrogen /
deuterium exchange (
HDX ) measurements . -
pubmed
Experiments carried out on
carboxypeptidase B in the absence of substrate and in the presence of saturating concentrations of
hippuryl - Arg result in HDX kinetics that are indistinguishable . -
pubmed
Immobility and lack of exercise , increased levels of proinflammatory
cytokines , increased production of
oxygen free radicals or impaired detoxification , low anabolic hormone output ,
malnutrition and reduced neurological drive have been advocated as being responsible for
sarcopenia . -
pubmed
It is reasonable to argue that the activity of these nodes results from the net balance of regulating mechanisms , as in the case of the
GH / IGF-1 axis , the
testosterone and
cortisol functions , the pro- and anti - inflammatory cytokines and receptors . -
pubmed
Rocuronium is an alternative to
mivacurium for short procedures , without the risk of unexpected prolonged relaxation due to a possible defect in
plasma cholinesterase . -
pubmed
MEASURES OF OUTCOME : Endpoints were serum lipid levels of total
cholesterol ,
HDL - C ,
LDL - C ,
VLDL - C ,
triglycerides and
apolipoproteins A-1 and B-100 . -
pubmed
No statistically significant differences were seen for total
cholesterol ,
triglycerides ,
LDL - C ,
VLDL - C ,
apolipoprotein A-1 or
apolipoprotein B-100 . -
pubmed
Serum total
cholesterol concentrations ,
low - density lipoprotein cholesterol concentrations , and liver function parameters decreased in the soy - based group and were significantly different from measurements in the traditional group ( P & lt ; 0.05 ) . -
pubmed
No significant change in serum
triacylglycerol levels , serum
high - density lipoprotein cholesterol levels , and fasting
glucose levels was found in the soy or traditional group . -
pubmed
CONCLUSION : Soy - based low - calorie diets significantly decreased serum total
cholesterol and
low - density lipoprotein cholesterol concentrations and had a greater effect on reducing body fat percentage than traditional low - calorie diets . -
pubmed
Calcium is involved in formation of high molecular weight
adiponectin . -
pubmed
CONCLUSION : These data indicate that
calcium promotes the formation of HMW
adiponectin , and
calcium sequestration decreases HMW
adiponectin . -
pubmed
Gain of interaction of
ALS - linked G93A
superoxide dismutase with cytosolic
malate dehydrogenase . -
pubmed
Protein interactions of the
Amyotrophic Lateral Sclerosis (
ALS)-linked
copper -
zinc superoxide dismutase (
hSOD1 ) G93A mutation were studied using a fluorescence resonance energy transfer ( FRET ) based screening system . -
pubmed
The FRET results confirmed by pull - down immunoprecipitation indicated " gain - of - interaction " of the G93A -
hSOD1 mutant with cytosolic
malate dehydrogenase (
cytMDH)-a key enzyme in the
malate -
aspartate shuttle which is vital to neurons . -
pubmed
Consistent with inhibition of the
malate -
aspartate shuttle , G93A -
hSOD1 had lower
malate and higher
lactate levels compared to non - induced or Wild - Type -
hSOD1 expressing cells . -
pubmed
Complex functions of
phosphatidylinositol 4,5 - bisphosphate in regulation of
TRPC5 cation channels . -
pubmed
The canonical
transient receptor potential (
TRPC ) proteins have been recognized as key players in
calcium entry pathways activated through
phospholipase - C - coupled receptors . -
pubmed
While it is clearly demonstrated that members of the
TRPC3 / 6 / 7 subfamily are activated by
diacylglycerol , the mechanism by which
phospholipase C activates members of the
TRPC1 / 4 / 5 subfamily remains a mystery . -
pubmed
In this paper , we provide evidence for both negative and positive modulatory roles for membrane
polyphosphoinositides in the regulation of
TRPC5 channels . -
pubmed
Depletion of
polyphosphatidylinositol 4 - phosphate and
phosphatidylinositol 4,5 - bisphosphate (
PIP2 ) through inhibition of
phosphatidylinositol 4 - kinase activates
calcium entry and membrane currents in
TRPC5 - expressing but not in
TRPC3- or
TRPC7 - expressing cells . -
pubmed
Inclusion of
polyphosphatidylinositol 4 - phosphate or
PIP2 , but not
phosphatidylinositol 3,4,5 - trisphosphate , in the patch pipette inhibited
TRPC5 currents . -
pubmed
Paradoxically , depletion of
PIP2 with a directed
5 - phosphatase strategy inhibited
TRPC5 . -
pubmed
Furthermore , when the activity of single
TRPC5 channels was examined in excised patches , the channels were robustly activated by
PIP2 . -
pubmed
These findings indicate complex functions for regulation of
TRPC5 by
PIP2 , and we propose that membrane
polyphosphoinositides may have at least two distinct functions in regulating
TRPC5 channel activity . -
pubmed
RCA - silenced plants were impaired in their attack - elicited
jasmonate ( JA)-
isoleucine (
Ile)/
leucine levels , but
RuBPCase - silenced plants were not , a deficiency that could not be restored by supplementation with
Ile or attributed to lower transcript levels of
JAR4 / 6 , the key enzyme for
JA - Ile conjugation . -
pubmed
From these results , we infer that
JA - Ile /
leucine signaling and the herbivore resistance traits elicited by JA -
Ile are influenced by
adenylate charge , or more generally ,
carbon availability in RCA- but not
RuBPCase - silenced plants . -
pubmed
Oligomerization of glycosyl -
phosphatidylinositol - anchored proteins (
GPI - APs ) into high molecular weight complexes is an essential step for their apical sorting in polarized epithelial cells . -
pubmed
Interestingly , when cells are depleted of
cholesterol and treated with
tunicamycin , treatments that by themselves do not affect
PLAP sorting ,
PLAP is not able to oligomerize and is missorted to the basolateral surface , thus supporting an indirect role of N - glycosylation , possibly mediated by a raft - associated glycosylated interactor . -
pubmed
In addition , the need for exogenous
insulin to maintain
glucose control has been reduced . -
pubmed
Proliferating cell nuclear antigen (
PCNA ) and
5 - bromo-2 '- deoxyuridine (
BrdU ) labeling index in normal liver graft reached the highest on postoperative day ( POD ) 3 , whereas in fatty grafts the expression peak was on POD 7 . -
pubmed
When compared with moderate MaS grafts B2 ( 70 % ROLT ) and mild MaS grafts A3 ( 60 % ) groups , the disparities of
alanine aminotransferase and total
bilirubin were significant . -
pubmed
9 - ODA is captured in the antennal lymph and transported to the pheromone receptor(s ) in the sensory neuron membranes by pheromone binding proteins (
PBPs ) . -
pubmed
A key issue is to understand how the
pheromone , once tightly bound to its
PBP , is released to activate the receptor . -
pubmed
This dimerization is disrupted by a unique residue mutation since
Asp35 Asn and
Asp35 Ala mutants remain monomeric at pH 7.0 , as does native
ASP1 at pH 4.0 . -
pubmed
Asp35 is conserved in only approximately 30 % of medium - chain
PBPs and is replaced by other residues , such as
Asn ,
Ala and
Ser , among others , thus excluding that they may perform domain swapping . -
pubmed
The amyloid - beta (
Abeta ) peptide , the 39 to 43
amino acid peptide that plays a substantial role in
Alzheimer ' s disease , has been shown to interact strongly with lipids both in vitro and in vivo . -
pubmed
Following the reaction with the ICAT
reagent , we applied one - dimensional gel electrophoresis with in - gel
trypsin digestion of proteins and subsequent MALDI -
TOF -
TOF mass spectrometric techniques for identification of peptides . -
pubmed
[ Preparation of monolithic
polylaurylmethacrylate column and its application in capillary electrochromatographic separation of
myoglobin digests ] . -
pubmed
Association of
CD69 up - regulation on
CD4 + Cla+ T cells versus patch test , strip patch test and clinical history in
nickel sensitization . -
pubmed
CONCLUSIONS :
CD69 up - regulation on CD4 + CLA+ T cells in vitro could not predict neither a positive PT or SPT result nor a positive clinical history to
nickel sulfate . -
pubmed
A novel assay employing
D - enantiomers of phospholipids as diluents for characterizing surface kinetics of lipid hydrolysis by
phospholipases is introduced . -
pubmed
Activity data show excellent agreement with a kinetic model derived with D - enantiomers as diluents and also that
D - phospholipids bind to the
enzyme but resist hydrolysis ; underscoring rationale ( i ) . -
pubmed
Computational and biological analyses of the
IL-27 binding site 1 to its receptor revealed important structural proximities with the
ciliary neurotrophic factor group of
cytokines and highlighted the contribution of
p28 Trp ( 97 ) , as well as of
EBI3 Phe(97 ) ,
Asp(210 ) , and
Glu(159 ) , as key residues in the interactions between both
cytokine subunits . -
pubmed
A study of
IL-27 binding site 3 showed that
Trp(197 ) was crucial for the
cytokine 's interaction with
gp130 , but that the mutated
cytokine still recognized
IL-27R on the cell surface . -
pubmed
BACKGROUND ,
AIM , AND SCOPE :
Methacrylates are
alpha , beta - unsaturated esters that are widely used in the polymer plastics and resins production . -
pubmed
Methylpyruvate ,
ethylpyruvate , and
butylpyruvate /
butanol were identified as main degradation products respectively in the
GC -
MS analysis . -
pubmed
The cardiac output ( Q(c ) ,
acetylene rebreathing ) response to exercise was determined from linear regression of Q(c ) and
VO ( 2 ) ( Douglas bags ) at
rest , ~30 % and ~60 % of peak VO(2 ) , and maximal exercise . -
pubmed
Tamoxifen , an antiestrogen used in the prevention and treatment of
breast cancer , is extensively metabolized by
cytochrome P450 enzymes . -
pubmed
Its biotransformation to
alpha - hydroxytamoxifen (
alpha - OHT ) , which may be genotoxic , and to
N - desmethyltamoxifen (
N - DMT ) , which is partially hydroxylated to
4 - hydroxy - N - DMT (
endoxifen ) , a potent antiestrogen , is mediated by
CYP3A enzymes . -
pubmed
We observed that the catalytic efficiency [ intrinsic clearance ( CL(int ) ) ] for
alpha - OHT formation with recombinant
CYP3A4 was 5 - fold higher than that with recombinant
CYP3A5 ( 0.81 versus 0.16 nl min-1 pmol
cytochrome P450 - 1 ) . -
pubmed
For
N - DMT formation , the CL(int ) with recombinant
CYP3A4 was only 1.7 - fold higher , relative to that with recombinant
CYP3A5 . -
pubmed
In addition , the CL(int ) for
N - DMT formation by
HLM with
CYP3A5 * 3 /* 3 alleles was approximately 3 - fold lower than that for
HLM expressing
CYP3A5 * 1 /* 1 . -
pubmed
Regression analyses of
tamoxifen metabolism with respect to
testosterone 6beta - hydroxylation facilitated assessment of
CYP3A5 contributions to the formation of the two metabolites . -
pubmed
The
CYP3A5 contributions to
alpha - OHT formation were negligible , whereas the contributions to
N - DMT formation ranged from 51 to 61 % . -
pubmed
Our findings suggest that polymorphic
CYP3A5 expression may affect the formation of
N - DMT but not that of
alpha - OHT . -
pubmed
The structures of the separated compounds were identified by EI -
MS ,
( 1 ) HNMR and
( 13 ) CNMR . -
pubmed
Here , we show that suppression of network activity with
tetrodotoxin , which increases surface expression of
AMPA receptors ( AMPARs ) , dramatically reduces levels of the deSUMOylating ( where SUMO is small ubiquitin - like modifier ) enzyme
SENP1 , leading to a consequent increase in protein SUMOylation . -
pubmed
Overexpression of the catalytic domain of
SENP1 prevents this scaling effect , and we identify
Arc as a SUMO substrate involved in the
tetrodotoxin - induced increase in AMPAR surface expression . -
pubmed
Synthesis and biological evaluation of
thiophene derivatives as
acetylcholinesterase inhibitors . -
pubmed
The
acetylcholinesterase inhibition activity was assayed according to Ellman 's method using
donepezil as reference . -
pubmed
Mechanisms of
Cisplatin - Induced Apoptosis and of
Cisplatin Sensitivity : Potential of
BIN1 to Act as a Potent Predictor of
Cisplatin Sensitivity in
Gastric Cancer Treatment . -
pubmed
Cisplatin forms inter- and intrastrand crosslinked DNA adducts and its
cytotoxicity is mediated by propagation of DNA damage recognition signals to downstream pathways involving
ATR ,
p53 ,
p73 , and
mitogen - activated protein kinases , ultimately resulting in apoptosis . -
pubmed
In addition , a new mechanism has recently been revealed , in which the oncoprotein
c - Myc suppresses
bridging integrator 1 (
BIN1 ) , thereby releasing
poly ( ADP - ribose ) polymerase 1 , which results in increased DNA repair activity and allows
cancer cells to acquire
cisplatin resistance . -
pubmed
The present paper focuses on the molecular mechanisms of
cisplatin - induced apoptosis and of
cisplatin resistance , in particular on the involvement of
BIN1 in the maintenance of
cisplatin sensitivity . -
pubmed
After culture for 1 , 12 , 24 and 48 h following IPL irradiation , fibroblasts and the vascular endothelial cell line were harvested for investigation of morphological changes by light microscopy , cell proliferation viability by
MTT assay , and
VEGF and
MMP secretions by ELISA . -
pubmed
AIM : To compare the effects of
telbivudine (
LDT ) and
entecavir (
ETV ) in treatment of
hepatitis B e antigen (
HBeAg)-positive
chronic hepatitis B by meta - analysis . -
pubmed
And the rate of increased
creatine kinase in the LDT group was higher than in the
ETV group ( RR 5.58 , 95 % CI 2.22 , 13.98 , P = 0.0002 ) . -
pubmed
The activity of
AtNUDX26 required
Mg2 + or
Mn2 + ions as cofactor and was optimal at pH 9.0 and 50 C. The expression of
AtNUDX26 and of
ppGpp metabolism - associated genes was regulated by various types of stress , suggesting that
AtNUDX26 regulates cellular ppGpp levels in response to stress and
impacts gene expression in
chloroplasts . -
pubmed
Dual
phosphoinositide 3 - kinase /
mammalian target of rapamycin inhibitor
NVP - BEZ235 has a therapeutic potential and sensitizes
cisplatin in
nasopharyngeal carcinoma . -
pubmed
To ascertain a therapeutical approach of
nasopharyngeal carcinoma (
NPC ) , we hypothesized
NVP - BEZ235 , a novel and potent
imidazo [ 4,5 - c ] quinolone derivative , that dually inhibits both
PI3 K and
mTOR kinases activities , had antitumor activity in
NPC . -
pubmed
In
NPC cell lines , with the extended exposure ,
NVP - BEZ235 selectively inhibited proliferation of
NPC cells harboring
PIK3CA mutation , compared to cells with wild - type
PIK3CA . -
pubmed
Furthermore , exposure of
NPC cells to
NVP - BEZ235 resulted in
G1 growth arrest by
Propidium iodide uptake assay , reduction of
cyclin D1and CDK4 , and increased levels of
P27 and
P21 by Western blotting , but negligible apoptosis . -
pubmed
Moreover , we found that
cisplatin (
CDDP ) activated
PI3K /
AKT and
mTORC1 pathways and
NVP - BEZ235 alleviated the activation by
CDDP through dually targeting
PI3 K and
mTOR kinases . -
pubmed
In combination with
NVP - BEZ235 and
CDDP , there was dramatic synergy in shrinking
tumor volumes and inducing apoptosis through increasing
Noxa ,
Bax and decreasing
Mcl-1 ,
Bcl-2 . -
pubmed
Subclinical
cardiovascular disease was expressed as
cIMT , carotid arterial stiffness and secondarily as a metabolic risk z - score including the homoeostasis model assessment score of
insulin resistance ,
triglycerides , total
cholesterol to
high - density lipoprotein ratio , inverse of cardiorespiratory fitness ,
systolic blood pressure and the sum of four skinfolds . -
pubmed
His - tagged
HP , incubated at pH 2.0 and 58 C , readily formed amyloid fibrils , as observed by thioflavin fluorescence , CD spectra , and transmission or atomic force microscopies . -
pubmed
Amyloid fibril was also readily formed from full - length
His -
HP when incubated with 10 - 20 %
2,2,2 - trifluoroethanol at pH 7.8 and 25 C without peptide bond cleavage . -
pubmed
Neutral
sugar content showed higher values in
SS ( 21.740 mg / mL ) and SL ( 18.657 mg / mL ) when compared with
KS ( 1.497 mg / mL ) and
KL ( 1.452 mg / mL ) ( p<0.05 ) . -
pubmed
Total
polyphenol contents of SL ( 423.139 mug / mL ) was significantly higher value ( p<0.05 ) than those of other samples , which occurred in the following order : SL> ;
KL ( 218.494 mug / mL)> ;
KS ( 107.269 mug / mL)> ;
SS ( 75.894 mug / mL ) . -
pubmed
KL exerted the highest
DPPH radical scavenging activity ( 84.834 % ) ( p<0.05 ) , which occurred in the following order : KL> ; SL ( 63.473%)> ;
KS ( 52.894%)> ;
SS ( 35.443 % ) . -
pubmed
ABTS radical scavenging activity showed that SL ( 66.088 % ) and
KL ( 38.511 % ) had higher scavenging activities , whereas
SS ( 7.695 % ) and
KS ( 9.609 % ) demonstrated to be lower activities ( p<0.05 ) . -
pubmed
Synthesis of
( 3 - hydroxy - pyrazolin-5 - yl ) glycine based ligands interacting with
ionotropic glutamate receptors . -
pubmed
These compounds were measured separately in the gas phase and the particle phase using a two - stage cartridge equipped with a
quartz fiber filter ( 1 mum mesh ) and
C18 solid - phase extraction disk for sampling and analyzed by
GC /
MS and LC /
MS /
MS ( for the detection of brominated flame retardants ) . -
pubmed
Among the compounds detected in this study , those with lower molecular weights tended to be captured in the
C18 solid - phase extraction disk rather than in the
quartz fiber filter . -
pubmed
Conditionally deleting
Sox2 from the population of nascent
cholinergic amacrine cells perturbed the normal ratio of cells situated in the
GCL versus the INL and induced a bistratifying morphology , with dendrites distributed to both ON and OFF strata within the inner plexiform layer . -
pubmed
A selective
5 - HT7 agonist ,
AS-19 , was totally ineffective in reducing S - IRA . -
pubmed
Transcripts predicting 15 putative neuropeptide prepropeptides ( (
allatostatin ,
allatotropin ,
bursicon alpha ,
corticotropin releasing factor (
CRF ) ,
CRF - binding protein ,
eclosion hormone ,
FMRFamide ,
glycoprotein A ,
insulin - like peptide , ion transport peptide ,
myoinhibitory peptide ,
inotocin ( =
neurophysin - oxytocin ) ,
Neuropeptide F ,
sulfakinin and
SIFamide ) ) and transcripts predicting receptors for 14 neuropeptides (
allatostatin , calcitonin , cardioacceleratory peptide ,
corazonin ,
CRF ,
eclosion hormone ,
gonadotropin - releasing hormone /
AKH - like ,
insulin - like peptide ,
neuropeptide F , proctolin ,
pyrokinin , SIFamide , sulfakinin and tachykinin ) are reported . -
pubmed
Additionally , transcripts predicting 6 neurotransmitter / neuromodulator receptors (
acetylcholine ,
GABA ,
dopamine ,
glutamate ,
octopamine and
serotonin ) and 3
neurotransmitter transporters (
GABA transporter ,
noradrenalin - norepinephrine transporter and
Na +- neurotransmitter / symporter ) are described . -
pubmed
RESULTS : SUA positively correlated with laboratory nutritional markers (
albumin ,
creatinine ) , body composition parameters ,
HGS ( r = 0.26 ; P & lt ; 0.001 ) and GNRI ( r = 0.34 ; P & lt ; 0.001 ) . -
pubmed
ROW1 specifically binds trimethylated histone
H3 lysine 4 ( H3K4me3 ) in the
WOX5 promoter region to repress its transcription . -
pubmed
Furthermore , the
carboxyl - terminal domain determines
myosin II localization in the constriction region of
PPT and is responsible for the specific role of
NMII in platelet release . -
pubmed
This inhibition was suppressed by silencing
IL-10R1 and was mimicked by
cobalt Protoporphyrin IX and
CO - releasing molecule-2 . -
pubmed
Taken together , our results suggest that
IL-10 /
HMOX1 signaling is involved in modulation of cochlear
inflammation through inhibition of
MCP-1 /
CCL2 regulation in SLFs , implying a therapeutic potential for a
CO - based approach for
inflammation - associated cochlear diseases . -
pubmed
Regression analysis was performed to look for any association of low
Mg0 with admission blood pressure ( BP ) and Glasgow Coma Scale (
GCS ) scores . -
pubmed
Mg0 levels were negatively associated with systolic BP presentation ( P & lt ; 0.0001 ) and positively associated with the initial
GCS scores ( P = 0.01 ) . -
pubmed
Mg levels on admission correlate inversely with systolic BP and directly with
GCS scores at presentation . -
pubmed
Furthermore , cerebrovascular
CO2 reactivity was assessed as a second metric of
CBF regulation by inducing
hypercapnia with 8 %
CO2 inhalation followed by
hyperventilation driven
hypocapnia . -
pubmed
The aim of this study was to confirm adjuvant chemotherapy of
HSS with
docetaxel plus
cisplatin (
DP ) against
NSCLC by evaluating antitumor activity and attenuated effect . -
pubmed
The patient resumed
proton pump inhibitor therapy (
esomeprazole ) without incident . -
pubmed
The dietxtimexinsulin resistance status interaction was not statistically significant in the model for overall
weight loss , although
insulin sensitive women at 12months lost more weight in the lower fat vs. lower
carbohydrate group ( 7.5 kg vs. 4.3 kg , P=0.06 ) , and in the walnut - rich vs. lower
carbohydrate group ( 8.1 kg vs. 4.3 kg , P=0.04 ) . -
pubmed
Sex hormone binding globulin increased within each group except in the lower
carbohydrate group at 12months ( P<0.01 ) . -
pubmed
Green cone opsin belongs to the
G protein - coupled receptor superfamily and consists of a seven transmembrane helical apoprotein covalently bound to
11 - cis - retinal , by means of a protonated
Schiff base linkage , in its inactive dark state . -
pubmed
A double
Cys mutant that could form a stabilizing
disulfide bond was used in an attempt to address the instability of the
green opsin mutants . -
pubmed
Renal
sodium handling and blood pressure changes in gestational protein - restricted offspring : Role of renal nerves and ganglia
neurokinin expression . -
pubmed
BACKGROUND : Considering long - term changes in renal
sodium handling and blood pressure in maternal protein - restricted (
LP ) offspring , we assumed that the development of LP
hypertension results from abnormal dorsal root ganglia ( DRG )
neurokinin expression associated with impaired responsiveness of renal sensory receptors , promoting a reduced urinary excretion of
sodium . -
pubmed
In addition , we assessed the tubular
sodium handling , estimated by
creatinine and
lithium clearances before and after bilateral renal denervation in conscious
LP offspring relative to age - matched
NP counterparts . -
pubmed
CONCLUSIONS : These observations raise the possibility that impaired DRG and pelvic
neurokinin expression associated with responsiveness of renal sensory receptors in 16 - wk old
LP offspring are conducive to excess renal reabsorption of
sodium and development of
hypertension in this programmed model . -
pubmed
None of the deletion mutants induced apoptosis as efficiently as full - length
RELT , a result that is consistent with a novel DD being located at the
carboxyl - terminus . -
pubmed
The inhibition of
mevalonate kinase (
MVK ) by downstream metabolites is an important mechanism in the regulation of
isoprenoid production in a broad range of organisms . -
pubmed
mazei
MVK , these
enzymes were not inhibited by
diphosphomevalonate (
DPM ) ,
dimethylallyl diphosphate (
DMAPP ) ,
isopentenyldiphosphate (
IPP ) ,
geranylpyrophosphate (
GPP ) or
farnesylpyrophosphate (
FPP ) . -
pubmed
However , they exhibited significantly higher affinity to
mevalonate and higher catalytic efficiency than the previously characterized
enzyme . -
pubmed
Benzo [ c ][ 1,2,5 ] thiadiazole derivatives : A new class of potent
Src homology-2 domain containing protein tyrosine phosphatase-2 (
SHP2 ) inhibitors . -
pubmed
Using scaffold - hopping strategy , a series of
benzo [ c ][ 1,2,5 ] thiadiazole derivatives was designed from
PTP1B inhibitors with
1H-2,3 - Dihydroperimidine motif , synthesized and evaluated their biological activities against
PTP1B and
SHP2 . -
pubmed
Characterization of a
nicotinic acetylcholine receptor binding site for
sulfoxaflor , a new
sulfoximine insecticide for the control of sap - feeding insect pests . -
pubmed
Through the evaluation of a set of
SFX analogs , we have demonstrated that displacement of [
3H]
SFX shows an excellent correlation with
GPA toxicity , and thus is toxicologically relevant . -
pubmed
Comparison with the previously described
methyl - SFX binding site information reveals differences with the
SFX binding site that are discussed herein . -
pubmed
In conclusion , CC homozygosis in the SNP - 174 G>C gene promoter of
IL-6 can be proposed as one of the gene variants influencing
iron accumulation in male adults with
HFE mutations . -
pubmed
RESULTS : The mean time taken to apply the
DMF was 3.8 min ; for ICDAS , it took 8.9 min , and for
CAST , 4.7 min . -
pubmed
When calculating the indicator the most severe
caries lesion per individual , the prevalence rates were as follows : 28.1 % for
DMF , 84.0 % for ICDAS and 75.0 % for
CAST . -
pubmed
CAST described disease distributions very well and identified lesion severities and preventive and curative needs in the examined group , and the time required to apply CAST was similar to that of the
DMF . -
pubmed
Therefore , the analytical results of biochemical indicators , histopathological observations and western blotting showed that
TLR and
DTG exhibited a significant effect in renal protection . -
pubmed
Furthermore ,
TLR and
DTG prevent high
glucose - induced glomerular mesangial cells ( GMCs ) by inhibiting
TGF - beta1 and Wnt /
beta - catenin signaling pathway , providing a powerful supports to develop a new therapeutic agent for
DN . -
pubmed
This finding shows that
N - glycans attached to
htau may affect its aggregation . -
pubmed
This system allowed us to investigate the effects of antisense
oligoribonucleotides representing functional domains of
guinea pig IFN - Alpha1 AS on gpIFNA1 mRNA levels and , consequently , on viral proliferation in the respiratory tract of
influenza virus - infected animals . -
pubmed
Our study provides evidence that
DSF inhibits
Aldehyde dehydrogenase (
ALDH ) enzyme activity , inhibits the expression of stemness - related transcription factors (
Sox ,
Nanog ,
Oct ) in
CSC derived from
breast cancer cell lines , and modulates intracellular reactive
oxygen species ( ROS ) generation . -
pubmed
Importantly , our research proved that
ALDH + stem - like cells play important roles in the resistance to the conventional chemotherapeutic agent
cisplatin . -
pubmed
DSF enhances the
cytotoxic effect of
cisplatin through inhibiting the stemness and by overcoming
cisplatin resistance of
ALDH + stem - like cells . -
pubmed
Further , we show that
ALDH +
cancer stem - like cells and ALDH- bulk
cancer cells have different intrinsic ROS levels , what may explain differences in susceptibility to
cisplatin treatment . -
pubmed
The results showed that
UFL1 depletion aggravated the LPS - induced inflammatory response and cell damage by positively regulating the
TLR4 /
NF - kappaB pathway ( increased the expression of
TLR4 ,
NF - kappaB P65 in nuclear , and
phospho -
IkappaBalpha ) , exacerbating LPS - induced
ER stress ( increased the expression of
CHOP ,
Hsp70 , and
GRP78 ) , apoptosis ( increased the expression of
Bax /
Bcl-2 and activity of
caspase-3 ) , autophagy ( increased
LC3 - II and decreased
P62 expression ) , and oxidative stress ( decreased
SOD and
CAT levels and increased
MDA levels ) . -
pubmed
Intracellular
FAS activity was measured by a spectrophotometer at 340 nm of
NADPH absorption . -
pubmed
Palmitic acid , the end product of
FAS catalyzed reaction , rescued the
ER stress and autophagy induced by
alpha - mangostin . -
pubmed
To solve these problems , a smart
albumin - based
tumor microenvironment - responsive nanoagent is designed via the self - assembly of
human serum albumin (
HSA ) , dc - IR825 ( a
cyanine dye and a photothermal agent ) , and
gambogic acid (
GA , a
heat shock protein 90 (
HSP90 ) inhibitor and an anticancer agent ) to realize molecular targeting - mediated mild - temperature PTT . -
pubmed
Here , we identify a
pilin peptidase iamP , and
LysR - type regulator (
LRTR )
iamR , that fulfil these roles and show that environmental
calcium , which oysters enrich for shell repair and growth , regulates
iam expression . -
pubmed
PiamR exhibited classical negative auto - regulation but , strikingly ,
IamR inversely regulated the divergent
iamP and
iamA promoters in a
calcium - dependent manner . -
pubmed
Moreover , expression of the
c - di - GMP and
calcium - regulated , biofilm - promoting
brp exopolysaccharide was
IamA - dependent . -
pubmed
These results support a scenario in which the
calcium - enriched oyster environment triggers
IamP - mediated processing of
prepilin amassed in the periplasm for rapid
pilin elaboration and subsequent
BRP production to promote colonization . -
pubmed
Conversely , the rhizosphere soil
acid phosphatase (
RS - APase ) activity was significantly higher ( 26 - 46 % ) in the IC treatments and occurred concomitant with a significant increase in available
phosphorus ( RS - Pavailable ) ( 26 - 74 % ) in the rhizosphere . -
pubmed
In the present study , the network pharmacology method was used to construct the ingredient - target network of
DHA that was responsible for the anti - HCC effect and 11 targets including
ALB ,
ATP5A1 ,
CCT3 ,
CLIC1 ,
ENO1 ,
HSPA8 ,
HSPB1 ,
NPM1 ,
PPIA ,
PRDX1 , and
ZYX were selected . -
pubmed
Further
lectin immunofluorescence and
lectin blot analysis showed that
DHA could down - regulate the expression of beta1,6 - branching of N - linked
carbohydrate by suppressing the transcription of
MGAT5 . -
pubmed
The target genes and active ingredients of
THH against RA comprised 64 genes and 17 compounds , the focus of which was
PTGS2 ,
triptolide , and
celastrol . -
pubmed
SystemsDock website indicated that the combing degree of
PTGS2 with
triptolide or
celastrol was very good . -
pubmed
The mechanisms of
THH against RA were linked to 31 signaling pathways , and the key mechanism was related to inhibition of
inflammation response through inactivating
TNF and
NF - kappa B signaling pathways . -
pubmed
Here , we report a route to access
metal - diamidobenzoquinone frameworks from their
dihydroxobenzoquinone counterparts via
post - synthetic linker exchange . -
pubmed
Combinatorial Roles of Mitochondria and
cGMP /
PKG Pathway in the Generation of Neuronal Free
Zn2 + under the Presence of
Nitric Oxide . -
pubmed
The molecular mechanism of
NZR has been extensively investigated , but the source of
Zn is present of a major candidate that is yet to be answered . -
pubmed
RESULTS : Two mechanisms , namely , mitochondria as the only
Zn source and the opening of
NO -
PKG - dependent mitochondrial
ATP - sensitive potassium channels ( mKATP ) as the key to releasing
NO - induced increase in mitochondrial
Zn , were proven to be the two critical paths of
NZR in neuronal - related cells . -
pubmed
The genes for the key
enzymes of the pathway were disrupted by insertion mutagenesis and the cellular metabolites in the
methanol extracts of mutant cells were analyzed by HPLC . -
pubmed
The deletion of the
doeA gene resulted in accumulation of the higher level of
ectoine compared to the wild type
strain . -
pubmed
Ngamma - acetyl - L-2,4 - diaminobutyrate (
Ngamma - acetyl - DAB ) , a substrate for
ectoine synthase , was found in the cytoplasm of the wild type
strain . -
pubmed
Nalpha - acetyl - L-2,4 - diaminobutyrate (
Nalpha - acetyl - DAB ) , a substrate for the
DoeB enzyme , appeared in the cells as a result of exposure of the
doeB mutant to low osmotic pressure . -
pubmed
The genes for the
enzymes involved in
ectoine degradation were found in all aerobic methylotrophs capable of
ectoine biosynthesis . -
pubmed
We have shown that
ectoine hydrolase DoeA in this methanotroph hydrolyzes
ectoine with the formation of the only isomer :
Nalpha - acetyl - DAB . -
pubmed
Moreover ,
p - hydroxycinnamic acid - based
Meldrum ' s derivatives possess valuable secondary activities sought by the cosmetic industry such as antioxidant and anti -
tyrosinase properties . -
pubmed
Following 12 months of supplementation with
resveratrol versus placebo , there were positive effects on bone density in the lumbar spine ( +0.016 + -0.003 g / cm2 ) and neck of femur ( +0.005 + -0.002 g / cm2 ) , which were accompanied by a 7.24 % reduction in C - terminal telopeptide type-1
collagen levels , a
bone resorption marker , compared to placebo . -
pubmed
Presented results clearly show the influence of
ZnO decomposition and segregation of
Zn atoms during evaporation and
post - deposition annealing on microstructure and optical properties of
zinc oxide films . -
pubmed
Derivatization of
Protein Crystals with
I3C using Random Microseed Matrix Screening . -
pubmed
Binding affinity and the capacity of the
amido analogues to stimulate the
phosphotransferase activity of the
kinases is greatly reeuced relative to
cAMP , the 3'-position being more sensitive towards the modification than the 5'-position . -
pubmed
The coordinate noncyclic derivatives ,
3 '- deoxy-3 '- amino-5 '- AMP (
IV ) and
5 '- deoxy-5 '- amino-3 '- amp ( iii ) , were also tested . -
pubmed
Surprisingly activity towards
protein kinases was found to be considerable for the
5 '- deoxy-5 '- amino-3 '- AMP ( III ) , while the
3 '- deoxy-3 '- amino-5 '- AMP (
IV ) is practically inactive . -
pubmed
The
phosphodiesterase splits both
cyclic amido analogues but with reduced rates compared to that of natural
cAMP . -
pubmed
Gestational diabetes was no less severe ( degree of
hyperglycaemia , need for
insulin therapy ) when associated with
norethisterone . -
pubmed
Acetylcholine ,
nicotine and the neuronal
nAChR agonist
dimethylphenylpiperazinium iodide (
DMPP ) , but not
muscarine , all evoked inward currents in the cells ( voltage - clamped at -60 mV ) . -
pubmed
DMPP 's actions were concentration- and voltage - dependent , and were antagonised by the neuronal
nAChR antagonist
mecamylamine ( 1 - 3 microM ) . -
pubmed
We have isolated from an HEL cell cDNA library an alternatively spliced transcript for the
platelet membrane glycoprotein IIb (
GPIIb ) that resulted from the deletion of the 34
amino acids of exon 28 of the GPIIb gene . -
pubmed
However , absence of the
carbohydrate appended to exon 28 did not prevent normal
GPIIb / IIIa heterodimer expression . -
pubmed
Our studies indicate that absence of the
amino acids encoded by GPIIb exon 28 sufficiently perturbs the quaternary configuration of the
GPIIb / IIIa heterodimer to impair its subsequent intracellular transport and processing . -
pubmed
to photolysis of caged
ATP and hydrolysis of
ATP , were separated from the absorbance changes due to the active
ATPase reflecting formation of the phosphorylated
Ca2E1P enzyme form . -
pubmed
Transmural nerve stimulation (
TNS ) induced a frequency - dependent increase in perfusion pressure in all vessels that was blocked by
tetrodotoxin ,
phentolamine , and
prazosin . -
pubmed
The inhibitory effect of endothelium was not modified by
indomethacin but was abolished by
hemoglobin , indicating that
endothelium - derived relaxant factor (
EDRF ) was the vasodilator involved . -
pubmed
The endothelium - dependent inhibitory effect ( rubbed vessel minus control vessel contractions ) increased with time during the first 10 - 20 s after the beginning of
TNS , and was frequency dependent and inhibited by low doses of
phentolamine , which suggest a receptor - mediated mechanism . -
pubmed
Effects of the
potassium channel blocking dendrotoxins on
acetylcholine release and motor nerve terminal activity . -
pubmed
Insulin had no effect on the capacity of erythrocytes to absorb
glucose . -
pubmed
Affinities to
estrogen ,
progesterone , and
androgen receptors , however , were very low . -
pubmed
Quantification is performed by single ion monitoring of
CAD species of
oxytetracycline and of its internal standard . -
pubmed
The effects of two
disulphides on
cholinesterase activity in the spectrophotometric assay . -
pubmed
5,5 '- Dithiobis -( 2 - nitrobenzoate ) did not influence serum
cholinesterase activity , whereas
2,2 '- dithiodipyridine had an inhibitory effect . -
pubmed
Tightly bound
oxalacetate and the activation of
succinate dehydrogenase . -
pubmed
Inhibition of
tyrosine hydroxylase by
pyrroloisoxazoles . -
pubmed
Enalapril maleate is unlike the prototype
angiotensin - converting enzyme inhibitor
captopril in that a standard meal does not appear to influence absorption of this new drug . -
pubmed
One of the siblings possessed only one of the relevant haplotypes , and was shown to be heterozygous for 21 - OH deficiency by the 17
alpha hydroxyprogesterone response to
ACTH stimulation . -
pubmed
When the cells were stimulated with PWM in the presence of a physiological concentration of
hydrocortisone ( HC ) , the secretion of
IgG was significantly higher in
HLA - B8 - positive individuals . -
pubmed
One type of aspiny neuron had an elongated , usually spindle - shaped , medium - size
soma with two , or rarely three , primary dendrites originating from opposite
poles of the cell ; one example of this type of neuron had two separate axons . -
pubmed
The possibility of characterizing subgroups of
depressive disorders by biological markers was studied by means of the
dexamethasone suppression test (
DST ) , the 24 - hr urinary free
cortisol ( UFC ) , the
growth hormone response to the
insulin tolerance test ( ITT ) , and polygraphic sleep recordings . -
pubmed
The substrate binding site of the
enzyme was found to consist of seven subsites , numbered -IV , -III , -II , -I , I ,
II and III , towards the subsite binding the reducing end unit of
xyloheptaose . -
pubmed
As a consequence of such an almost symmetric distribution of affinities around the catalytic groups , the
enzyme cleaves preferentially the bonds in the
oligosaccharides which are most distant from both terminals . -
pubmed
A hypothesis is discussed according to which only type I cells are primarily responsive to
PHA , whereas type
II cells require " help " from the former , prior to transition into the proliferative stage . -
pubmed
The transport activity for
cystine was enhanced when the cells were incubated with
tumour necrosis factor - alpha (
TNF - alpha ) , but not with
interferon - gamma (
IFN - gamma ) or
interleukin-1 . -
pubmed
The
IGF I receptor (
IGF IR ) is a heterotetramer composed of two cross - linked extracellular alpha - chains and two membrane - spanning beta - chains that contain a
tyrosine - kinase domain . -
pubmed
It has a high degree of sequence similarity to the
insulin receptor (
IR ) , and the putative ligand - specific binding site has been localized to a
cysteine - rich region ( CRR ) of the alpha - chain . -
pubmed
A polyclonal antibody directed against a 48 -
amino acid synthetic peptide , corresponding to a region of the CRR postulated to be essential for ligand binding , failed to react with either solubilized , reduced or intact
IGF IR . -
pubmed
The extracellular
amino - terminal part ,
TM IV and the second extracellular loop contain several replacements suggesting that these portions have no or limited direct interactions with the peptide ligands . -
pubmed
Effect of
oxygen abstraction on the
peroxidase -
luminol -
perborate system : relevance to the
HRP enhanced chemiluminescence mechanism . -
pubmed
Abstraction of
oxygen from the
HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated . -
pubmed
It is , therefore , proposed that in the
peroxidase -
luminol - perborate system at pH 7.3 , chemiluminescence is generated by a direct reaction of
diazaquinones with
hydrogen peroxide and not , as generally assumed , from the reaction of
luminol radicals with the molecular
oxygen . -
pubmed
Significantly higher frequencies of " disadvantage " alleles , usually combined with a higher
LDL cholesterol , were found in hypercholesterolemic individuals when polymorphisms in
apolipoprotein E ,
apolipoprotein B -- XbaI and
LDL receptor --
PvuII were studied . -
pubmed
Although the histogenesis of these intranuclear inclusions has not been clarified , they should not be mistaken for those in endometrial cells infected by herpesvirus because of intranuclear
biotin and a false - positive histochemical reaction in the
avidin -
biotin -
peroxidase complex method . -
pubmed
Iba1 protein is therefore suggested to act as an adapter
molecule , mediating
calcium signals that may function in a monocytic lineage including microglia . -
pubmed
Increasing
4 '- epidoxorubicin and fixed
ifosfamide doses plus
granulocyte - macrophage colony - stimulating factor in advanced
soft tissue sarcomas : a pilot study . -
pubmed
PURPOSE : To determine the maximum - tolerated dose ( MTD ) of
4 '- epidoxorubicin (
EPI ) in combination with full dose of
ifosfamide (
IFO ) when
granulocyte - macrophage colony - stimulating factor (
GM - CSF ) was used , to estimate its clinical efficacy , and to evaluate the mobilization of hematopoietic progenitors . -
pubmed
The most relevant mobilization effect was obtained at the third
EPI level , when both
GM - CSF and
IL-3 were used as in vitro - stimulating factors . -
pubmed
It is commonly assumed that the role of the strongly activated heterosynaptic input during the induction of associative long - term potentiation ( LTP ) is to relieve the
magnesium blockade of
NMDA receptors located at the weakly stimulated synapses and thereby allow the weak input to undergo potentiation . -
pubmed
The effect of releasing
D - AP5 by flash photolysis of 100 microM caged
D - AP5 (
N -[ 1 -( 2 - nitrophenyl ) ethoxycarbonyl ]- D - AP5 ) on pharmacologically isolated
NMDA receptor - mediated field EPSPs was examined in area
CA1 . -
pubmed
Rapid application of
D - AP5 , by flash photolysis of caged
D - AP5 , coincident with the last
shock of the strong
tetanus , resulted in the blockade of
NMDA receptor activation during the period of the weak
tetanus . -
pubmed
MMPs are a homologous family of
zinc - dependent
proteases . -
pubmed
PVNx also lowered the depressor response to ganglionic blockade ( AL - PVNx=-28+/-5 mmHg ,
FR - PVNx=-29+/-4 mmHg ) and plasma
norepinephrine levels ( AL - PVNx=372+/-74 pg / ml ,
FR - PVNx=248+/-31 pg / ml ) . -
pubmed
Even though protection at the membrane level is conferred by the
nitroxides during the early stages of lipid peroxidation , their antioxidative ability might be overwhelmed at a later stage by other mechanisms such as the increased autoxidation of
hemoglobin in the presence of the
nitroxides , thus giving a possible explanation for the early induction of
hemolysis induced by the
nitroxides . -
pubmed
Finally , using in vitro binding studies , we showed that
SREBP2 was able to displace
ADD1 /
SREBP1 binding from the
sterol regulatory element ( SRE ) site . -
pubmed
HAT activities acetylating either histone
H3 (
SAGA ,
Ada , and
NuA3 ) or
H4 (
NuA4 ) stimulate HIV-1 transcription from preassembled nucleosomal templates in an
acetyl CoA - dependent manner . -
pubmed
His ...
Asp catalytic dyad of
ribonuclease A : conformational stability of the wild - type , D121N , D121A , and H119A
enzymes . -
pubmed
Residue
His119 acts as an acid / base during the cleavage / hydrolysis reactions catalyzed by
bovine pancreatic ribonuclease A (
RNase A ) . -
pubmed
This
His ...
Asp dyad is conserved in all homologous pancreatic
ribonucleases of known sequence . -
pubmed
Accordingly ,
Asp121 was found to have pKa values of approximately 2.4 and 3.4 in the native and denatured states , respectively , of wild - type
RNase A. -
pubmed
We conclude that the
His ...
Asp catalytic dyad in pancreatic
ribonucleases has two significant roles : ( 1 ) to position the proper tautomer of
His119 for catalysis and ( 2 ) to enhance the conformational stability of the native enzyme . -
pubmed
Asp121 of
RNase A is a rare example of a residue that contributes equally to both . -
pubmed
If catch - up growth can not be achieved by an alternate - day
steroid regimen and discontinuation of glucocorticoids appears to be an intolerable risk for graft survival ,
rhGH therapy may be initiated . -
pubmed
Co - expression of
CD7 on
CD34 + cells was induced to decrease significantly after short - term in vitro culture with the differentiation - inducing agent
phorbol ester (
PMA ) and with a combination of
cytokines (
stem - cell factor ,
interleukin-3 and
granulocyte colony - stimulating factor ) . -
pubmed
The loss of
CD7 marker following incubation with
PMA and
cytokines suggests that this antigen is expressed transiently in early myeloid leukaemic
CML haemopoiesis . -
pubmed
Differential effects of
cocaine and
methamphetamine on
neurotensin / neuromedin N and
preprotachykinin messenger RNA expression in unique regions of the striatum . -
pubmed
This study employed in situ hybridization to directly compare the effects of
cocaine and
methamphetamine on
neurotensin / neuromedin N and
preprotachykinin messenger RNAs in distinct striatal regions . -
pubmed
Methamphetamine and
cocaine produced significant increases in
preprotachykinin messenger RNA in the striatum after 3h , but often in different subregions . -
pubmed
However ,
methamphetamine produced significant increases in all regions of the caudal striatum , whereas
cocaine - induced
preprotachykinin messenger RNA expression was limited to dorsal regions of this striatal area . -
pubmed
Methamphetamine also produced a significant increase in
preprotachykinin messenger RNA in the caudal striatum after 30min , whereas
cocaine had no significant effect on
preprotachykinin messenger RNA at this early time - point . -
pubmed
The pattern of changes in
neurotensin /
neuromedin N messenger RNA caused by
methamphetamine and
cocaine after 3h was even more distinct . -
pubmed
Cocaine produced significant increases in
neurotensin / neuromedin N messenger RNA in all regions of the rostral striatum , whereas
methamphetamine had no effect in these areas . -
pubmed
Furthermore , in more caudal sections ,
cocaine predominantly affected
neurotensin / neuromedin N expression in dorsal aspects of the striatum , whereas
methamphetamine significantly increased
neurotensin / neuromedin N messenger RNA in all regions . -
pubmed
The only significant effect was an increase in
neurotensin / neuromedin N messenger RNA in the core region 3h after
methamphetamine administration . -
pubmed
These results indicate that
methamphetamine and
cocaine increase
preprotachykinin and
neurotensin / neuromedin N messenger RNAs in distinct regions of the striatum . -
pubmed
Internalization of these variant receptors was dependent on the catalytic
cysteine residue of
Rsp5p and on ubiquitin - conjugating enzymes that bind
Rsp5p . -
pubmed
The crystal structure of the complex of the
COOH - terminal fragment of the heavy chain with an analogue of its
ganglioside receptor , GT1b , provides the first direct identification and characterization of the
ganglioside - binding sites . -
pubmed
The appearance of
p32 was assessed by immunoblotting
SDS - extracted and separated sperm proteins using an anti - phosphotyrosine antibody . -
pubmed
An in - gel
tyrosine kinase renaturation assay showed that
TK-32 activity depends on
calcium and
bicarbonate in the incubation medium . -
pubmed
Celecoxib treatment inhibited
VEGF mRNA expression without any significant reduction in
cyclooxygenase-2 mRNA . -
pubmed
Thus ,
celecoxib reaches the retina after oral administration and reduces
diabetes - induced retinal
VEGF mRNA expression and vascular leakage by inhibiting the activity of
cyclooxygenase-2 enzyme . -
pubmed
SU5416 is reported to be a selective inhibitor of
vascular endothelial growth factor , and it has metwith limited success in the clinic . -
pubmed
Treatment of the
ovarian carcinoma cells with
SU5416 was also associated with a reduced expression of
ERCC-1 protein and c - jun mRNA , as well as a decrease in
c - Jun and
JNK activities . -
pubmed
The interconversion of 1 into 2 - 4 , as well as
[ ( ( t ) Bu )( 2 ) Ga ( mu - OPh )]( 2 ) into 5 , and their reverse reactions , have been followed by
( 13 ) C CPMAS NMR spectroscopy ,
TG / DTA , SEM , EDX , and powder XRD . -
pubmed
STUDY ON THE BIOGENESIS OF
CATECHOLAMINES IN PHEOCHROMOCYTOMA TISSUE CULTURE . -
pubmed
The recombinant
alr0975 protein strongly catalyzed the first step of PC synthesis , in which
glutathione (
GSH ) is converted to
gamma - glutamylcysteine (
gamma - EC ) , although the protein only weakly catalyzed the second step of PC synthesis , namely the transfer of
gamma - EC moiety to an acceptor
GSH molecule to form
PC ( 2 ) . -
pubmed
Phosphotyrosyl peptides and analogues as substrates and inhibitors of
purple acid phosphatases . -
pubmed
Based on the observation that
phosphotyrosine - containing peptides are good substrates for
pig purple acid phosphatase , peptides containing a range of
phosphotyrosine mimetics were synthesized . -
pubmed
HR -
TEM ( high - resolution transmission electron microscopy ) and selected - area EDS ( energy - dispersive spectroscopy ) show that the nanoparticles are homogenously spread in the
ZnO tubes . -
pubmed
Effect of crosslinking and drug loading on thermal decomposition of chitosan was studied by thermogravimetry (
TGA ) and these data indicated that pure chitosan is stable when compared to
clozapine - loaded chitosan . -
pubmed
OBJECTIVE : A missense mutation at codon 810 (
Ser --> ;
Leu ) of the
mineralocorticoid receptor was recently observed in a family with early manifestation of
hypertension . -
pubmed
Peak blood
glucose (
BG ) and time to peak
BG during PICU admission , duration of
hyperglycemia ( percentage of PICU days with any
BG of & gt;126 mg / dL ) , and intensity of
hyperglycemia ( median
BG during first 48 PICU hours ) were analyzed for association with PICU mortality using chi - square , Student 's t - test , and logistic regression . -
pubmed
Impact of in
ovo - administered lead and
testosterone on developing female thymocytes . -
pubmed
Using an avian in
ovo model of lead - induced T - helper disruption , the ability of in
ovo administered lead and
testosterone to alter thymocyte maturation among female embryos was investigated . -
pubmed
However , when
testosterone treatment was followed by lead (
testosterone + lead ) , there was a significant increase in
CD4+
CD8 + double - positive cells compared with either control or lead treatment groups . -
pubmed
Testosterone , either by itself or in combination with lead , significantly reduced the percentage of cells with the
CD4+
CD8- phenotype when compared to the lead alone group . -
pubmed
We measured the gain of the aVOR elicited by rapid rotary head thrusts in each of the canal planes for each subject before and after treatment with intratympanic
gentamicin by using magnetic search
coils to record eye movements . -
pubmed
Modification of
GTPases with
isoprenoid molecules derived from
geranylgeranyl pyrophosphate or
farnesyl pyrophosphate is an essential requisite for cellular signaling pathways . -
pubmed
Both enzymes play crucial roles in cell survival , and inhibition of
farnesyl pyrophosphate synthase by
nitrogen - containing
bisphosphonates is an established concept in the treatment of
bone disorders such as
osteoporosis or certain forms of
cancer in bone . -
pubmed
Our experiments demonstrate that a
platinum cross - link locally overrides the rotational setting predefined in the nucleosome positioning sequence such that the lesion faces toward the
histone core . -
pubmed
The
pifi mutant exhibited a lower capacity for nonphotochemical quenching , but similar
CO ( 2 ) assimilation rates ,
photosystem II (
PSII ) quantum efficiencies ( PhiPSII ) , and reduction levels of the primary electron acceptor of
PSII ( 1 - qL ) as compared with the wild type . -
pubmed
Erythrocyte activity of
transketolase , the concentrations of
thiamine and related phosphorylated metabolites in plasma , erythrocytes and urine , and markers of metabolic control and
vascular dysfunction were determined . -
pubmed
Plasma
thiamine concentration and urinary excretion of
thiamine correlated negatively with soluble
vascular adhesion molecule-1 ( r = -0.246 , p & lt ; 0.05 , and -0.311 , p & lt ; 0.01 , respectively ) . -
pubmed
The conventional assessment of
thiamine status was masked by increased
thiamine transporter content of erythrocytes . -
pubmed
Ornithine decarboxylase (
ODC ) , the rate - limiting enzyme in
polyamine biosynthesis that decarboxylates
ornithine to
putrescine , has become a promising target for
cancer research . -
pubmed
RESULTS :
PI3 K inhibitors
wortmannin and
LY294002 caused dose - dependent cellular and mitochondrial
GSH depletion and downregulation of the modulatory subunit of
GCL in cultured
RPE cells . -
pubmed
Both the basal and the induced
Nrf2 activities were inhibited by
wortmannin and
LY294002 . -
pubmed
LY294002 also inhibited
sulforaphane - induced
Nrf2 nuclear translocation . -
pubmed
Samples of cortical tissue collected 24 h
post METH treatment were pooled , processed and analyzed via a selective psychoproteomic platform . -
pubmed
The
beta - ketoacyl -( acyl carrier protein ) synthases (
beta - keto - ACP synthases ;
KAS ) catalyse the addition of two -
carbon units to the growing
acyl chain during the elongation phase of
fatty - acid synthesis . -
pubmed
The dimeric state of
TtKAS II is important to create each
fatty - acid - binding pocket . -
pubmed
Closer examination of
KAS structures reveals that compared with other
KAS structures in the apo form , the active site of
TtKAS II is more accessible because of the ; open ' conformation of the
Phe396 side chain . -
pubmed
The cardinal features of
AD include the formation of extracellular protein deposits in the brain that consist predominantly of aggregates of b amyloid protein(
senile plaques ) , neurofibrilary tangles (
hyperfosforylated tau protein ) in the intracellular compartments , disturbances in
calcium homeostasis , and degeneration / loss of synapses and neurons . -
pubmed
Chronic activation of the microglia and astrocytes may cause damage of the brain - blood barrier and
neuronal damage through the release of potentially cytotoxic molecules such as proinflammatory
cytokines , reactive
oxygen species ,
NO , and complement proteins . -
pubmed
Gonadal
steroid production is stimulated by
gonadotropin binding to
G protein - coupled receptors (
GPCRs ) . -
pubmed
Although
GPCR - mediated increases in intracellular
cAMP are known regulators of steroidogenesis , the roles of other signaling pathways in mediating
steroid production are not well characterized . -
pubmed
This pathway is critical for
LH - induced
steroid production in ovarian follicles , probably through
matrix metalloproteinase (
MMP)-mediated release of
EGF receptor (
EGFR ) binding ectodomains . -
pubmed
We demonstrated that , similar to the ovary , trans - activation of the
EGF receptor was critical for
gonadotropin - induced
steroid production in Leydig cells . -
pubmed
LH - induced increases in
cAMP and
cAMP - dependent protein kinase (
PKA ) activity mediated trans - activation of the
EGF receptor and subsequent
mitogen - activated protein kinase (
MAPK ) activation , ultimately leading to
StAR phosphorylation and mitochondrial translocation . -
pubmed
Steroidogenesis in Leydig cells was unaffected by
MMP inhibitors , suggesting that
cAMP and
PKA trans - activated
EGF receptors in an intracellular fashion . -
pubmed
Interestingly , although
cAMP was always needed for steroidogenesis , the
EGFR /
MAPK pathway was activated and necessary only for early ( 30 - 60 min ) , but not late ( 120 min or more ) ,
LH - induced steroidogenesis in vitro . -
pubmed
Responses of limbic and extrapyramidal
substance P systems to
nicotine treatment . -
pubmed
RESULTS : The
nicotine treatment significantly but temporarily decreased
substance P - like immunoreactivity ( SPLI ) content in the ventral tegmental area ( VTA ) and substantia nigra 12 - 18 h after drug exposure . -
pubmed
The
nicotine - mediated changes in SPLI were selectively blocked by pretreatment with
mecamylamine as well as a
dopamine D1 ,
D2 , or both receptor antagonists . -
pubmed
CONCLUSIONS : These findings indicate that some limbic and basal ganglia SP systems are significantly affected by exposure to
nicotine through processes mediated by
nicotinic and dopaminergic receptors , suggesting a role for SP pathways in
nicotine 's limbic and extrapyramidal effects . -
pubmed
The responsive
protein adsorption on four
polyCBAAs was mapped out . -
pubmed
MS medium supplemented with 1.5 mg L(-1 ) BA and 0.2 mg L(-1 )
NAA elicited the maximum number of shoots ( 4 multiple shoots ) from
nodal explants . -
pubmed
These adventitious shoots were best rooted on half strength
MS medium supplemented with 1.0 mg L(-1 ) each of
IBA and
IAA . -
pubmed
The
pan - caspase inhibitor (
z - VAD - fmk ) completely blocked the
pFPhe - mediated apoptotic changes except
caspase-9 activation . -
pubmed
Such findings confirm the importance of
CTGF /
CCN-2 in development and of the necessary and sufficient role of this
molecule in formation of the skeleton , extracellular matrix and chondrogenesis . -
pubmed
Acid polysaccharide - induced amorphous
calcium carbonate (
ACC ) films : colloidal nanoparticle self - organization process . -
pubmed
Amorphous
calcium carbonate (
ACC ) plays important roles in biomineralization , and its synthesis in vitro has been of keen interest in the field of biomimetic materials . -
pubmed
We extended previously developed quantitation of thoracic fat volume ( TFV ) from non - contrast coronary
calcium ( CC )
CT scans to also quantify pericardial fat volume ( PFV ) and investigated the associations of PFV and TFV with CC and the
Metabolic Syndrome ( METS ) . -
pubmed
PFV and
TFV were also associated with METS [ PFV : p<0.001 , OR 6.1 ; TFV p<0.001 , OR 5.7 ] , unlike CC [ OR=1.0 p =
NS ] or RF . -
pubmed
PFV correlated with low - HDL and high -
glucose ; TFV correlated with low -
HDL , low -
adiponectin , and high
glucose and
triglyceride levels . -
pubmed
Calcitonin - stimulated renal
Ca2 + reabsorption occurs independently of
TRPV5 . -
pubmed
BACKGROUND :
Calcitonin (
CT ) is known to affect renal
Ca ( 2 + ) handling . -
pubmed
However , it remains unclear how
CT affects
Ca ( 2 + ) transport in the distal convolutions . -
pubmed
The aim of this study was to investigate the contribution of the renal epithelial
Ca ( 2 + ) channel ,
transient receptor potential vanilloid 5 (
TRPV5 ) , to renal
Ca ( 2 + ) handling in response to
CT . -
pubmed
In addition , urinary
Na (+ ) and
K (+ ) excretion also decreased after
CT administration . -
pubmed
Transient exposure of cardiac myocytes to
hydrogen peroxide (
H ( 2 ) O ( 2 ) ) results in further production of
superoxide by the mitochondria as a result of increased influx of
calcium through the
L - type Ca ( 2 + ) channel and increased
calcium uptake by the mitochondria . -
pubmed
Exposure of myocytes to 30 muM
H ( 2 ) O ( 2 ) ( 5 min ) then 10 U / mL
catalase ( 5 min ) increased
dihydroethidium (
DHE ) signal by 58.7 + - 12.0 % ( n=4 ) compared to myocytes exposed to 0 muM
H ( 2 ) O ( 2 ) for 5 min followed by 10 U / mL
catalase ( n=9 ) . -
pubmed
Complex I inhibitors
DPI ( n=5 ) and
rotenone ( n=7 ) attenuated the increase in
DHE signal due to
H ( 2 ) O ( 2 ) . -
pubmed
However ,
antimycin A ( inhibitor of Q(i ) site of
complex III ) had no effect . -
pubmed
Direct activation of the
L - type Ca ( 2 + ) channel by voltage - step mimicked the increase in
DHE signal after transient exposure to
H ( 2 ) O ( 2 ) ( 47.6 + - 17.8 % , n=6 ) while intracellular application of
catalase attenuated the increase in
DHE signal due to
H ( 2 ) O ( 2 ) ( n=6 ) . -
pubmed
In pairwise comparisons of complete genomic sequences and ORF
C1 nucleotide and
amino acid sequences , DuCVs of the same genotype were clearly distinguished from those of heterologous genotypes . -
pubmed
Analysis of the
amino acid sequences of the
capsid protein exhibited the existence of 18 genotype - specific substitutions . -
pubmed
Four of the novel
HLA - C alleles encode
amino acid changes at codons 41 , 42 , 55 or 200 which have not previously been reported to be polymorphic . -
pubmed
Some of the new alleles encode novel codons and unique
amino acid changes at polymorphic positions in the
HLA - A ( codons 55 and 120 ) ,
HLA - C ( codons 151 , 153 and 176 ) ,
HLA - B ( codons 31 and 84 ) and
HLA - DRB1 ( codon 47 ) loci . -
pubmed
Astrocytes at
seizure foci seem to not be a homogeneous population of cells , and in addition to typical
glial fibrillary acidic protein , positive reactive astrocytes also include a population of neuron glia-2 - like cells The astrocytes in sclerotic hippocampi differ from those in nonsclerotic hippocampi in their membrane physiology , having elevated
Na + channels and reduced inwardly rectifying
potassium ion channels , and some having the capacity to generate action potentials . -
pubmed
Furthermore , the CC genotype of the c.891C>T polymorphism in the
GPX1 gene was associated with higher values of
8 - oxo - dG and
GPX activity levels as compared to those for the
CT - TT genotype . -
pubmed
Liquorice and
glycyrrhetinic acid increase
DHEA and
deoxycorticosterone levels in vivo and in vitro by inhibiting adrenal
SULT2A1 activity . -
pubmed
The mineralocorticoid effects of liquorice are mediated by the inhibitory effects of one of its active components
glycyrrhetinic acid on
11beta - hydroxysteroid dehydrogenase type 2 . -
pubmed
Here we have investigated the wider effects of oral liquorice on steroidogenesis focussing particularly on possible inhibitory effects of
glycyrrhetinic acid on
adrenal sulfotransferase activity . -
pubmed
Cortisol and
cortisone levels reflected expected inhibition of
11beta - hydroxysteroid dehydrogenase type 2 by
glycyrrhetinic acid . -
pubmed
Glycyrrhetinic acid inhibited
cortisone and enhanced
cortisol synthesis consistent with
11beta - hydroxysteroid dehydrogenase type 2 inhibition . -
pubmed
Inhibition of
deoxycorticosterone and
dehydroepiandrosterone conjugation was apparent within 4 h of starting
glycyrrhetinic acid treatment and was not associated with changes in the expression of
SULT 2A1 mRNA . -
pubmed
Herein , we consider
PAL once more , this time testing ISM as a means to broaden the substrate scope of this
lipase by studying bulky substrates of the type
2 - phenylalkanoic acid esters as substrates that are not accepted by the WT . -
pubmed
Iron administered for treatment of
anaemia resulted in a larger increase in
haemoglobin than
iron given for prevention , and the benefit was similar in hyper- or holoendemic and lower endemicity settings . -
pubmed
Temperature dependent spectral properties of type - I and quasi type -
II CdSe /
CdS dot - in - rod nanocrystals . -
pubmed
Megalencephalic leukoencephalopathy with
subcortical cysts type 1 (
MLC1 ) due to a homozygous deep intronic splicing mutation ( c.895 - 226T > G ) abrogated in vitro using an antisense
morpholino oligonucleotide . -
pubmed
Its circulating concentration is maintained within a very tight physiologic range : 2.25 and 2.50 mmol / L. Under physiological conditions , the ionized
calcium concentration is regulated by the
parathyroid hormone (
PTH ) , and
1,25 ( OH )( 2 ) vitamin D through interactions on target organs such as kidney , bone and intestine . -
pubmed
The main tools are specific standard biochemical tests able to assess
calcium balance and renal function , and some specific biochemical tests , such as
PTH ,
25 ( OH ) vitamin D , and genetic sequencing , used to clarify the cause of
hypercalcemia and , subsequently , to determine appropriate therapy . -
pubmed
Once
hypercalcemia is confirmed by ionized
calcium measurement , the intact
PTH assay plays a crucial role to differentiate
PTH - mediated from non -
PTH - mediated
hypercalcemia . -
pubmed
It is based on the oxidation of
tetramethylbenzidine (
TMB ) , which is catalysed by
haemoglobin and its derivatives . -
pubmed
Bax and
Bak are required for
apogossypolone , a BH3 - mimetic , induced apoptosis in
chronic lymphocytic leukemia cells . -
pubmed
Non - canonical
Smads phosphorylation induced by the
glutamate release inhibitor ,
riluzole , through
GSK3 activation in
melanoma . -
pubmed
In the present study , we demonstrate that
riluzole inhibits
AKT - mediated
glycogen synthase kinase 3 (
GSK3 ) phosphorylation in
melanoma cell lines . -
pubmed
Because we have demonstrated that
GSK3 is involved in the phosphorylation of two downstream effectors of
transforming growth factor beta (
TGFbeta ) ,
Smad2 and
Smad3 , at their linker domain , our aim was to determine whether
riluzole could induce
GSK3beta - mediated linker phosphorylation of
Smad2 and
Smad3 . -
pubmed
We present evidence that
riluzole increases
Smad2 and
Smad3 linker phosphorylation at the cluster of
serines 245/250/255 and
serine 204 respectively . -
pubmed
Using
GSK3 inhibitors and siRNA knock - down , we demonstrate that the mechanism of
riluzole - induced
Smad phosphorylation involved
GSK3beta . -
pubmed
The
riluzole - induced
Smad linker phosphorylation is mechanistically different from the
Smad linker phosphorylation induced by
TGFbeta . -
pubmed
We further show that
riluzole upregulates the expression of
INHBB and
PLAU , two genes associated with the
TGFbeta signaling pathway . -
pubmed
The non - canonical increase in
Smad linker phosphorylation induced by
riluzole could contribute to the modulation of the pro - oncogenic functions of
Smads in late stage
melanomas . -
pubmed
Here , we report the pharmacological activity of a 39
amino acid CNP analog (
BMN 111 ) with an extended plasma half - life due to its resistance to
neutral - endopeptidase (
NEP ) digestion . -
pubmed
' One -
pot ' synthesis of
dihydrobenzo [ 4,5 ][ 1,3 ] oxazino [ 2,3 - a ] isoquinolines via a
silver ( I )-catalyzed cascade approach . -
pubmed
The strategy features an
AgNO ( 3 ) catalyzed ' one -
pot ' cascade process involving formation of two new
C - N bonds and one new
C - O bond . -
pubmed
In addition , the contractile response induced by 1 muM
phenylephrine increased from 6.8 + - 0.6 mN to 11.4 + - 0.4 mN ( P & lt ; 0.05 ) and 14.9 + - 1.4 mN ( P & lt ; 0.05 ) by
insulin ( 1 IU / kg , intraperitoneal ) or
CAPA treatment , respectively . -
pubmed
In Group A , after producing
fatigue , P di at 20 Hz stimulation increased significantly with a bolus injection ( 0.75 mg kg(-1 ) ) followed by continuous infusion of
amrinone ( 2.5 , 5 and then 10mug kg(-1)min(-1 ) )
IV ( P<0.05).P di at 100 Hz stimulation increased significantly with an administration of
amrinone ( 10mug kg(-1)min(-1 )
IV ( P<0.05 ) . -
pubmed
Self - assembly structures of
1H - indazoles in the solution and solid phases : a vibrational ( IR ,
FIR , Raman , and VCD ) spectroscopy and computational study . -
pubmed
Using broad - spectrum and specific
MMP inhibitors , we establish that
MMP-1 , but not
MMP-2 and - 9 , plays a key role in
5 - azadC - enhanced cell invasion . -
pubmed
We show that
5 - azadC induces
MMP-1 expression through a transcriptional mechanism without affecting
MMP-1 promoter methylation status . -
pubmed
Finally , we demonstrate that
5 - azadC treatment increases the nuclear levels of
Sp1 and
Sp3 transcription factors , and modulates their recruitment to the
MMP-1 promoter , resulting in chromatin remodeling associated to
5 - azadC - induced
MMP-1 expression . -
pubmed
MOD and
MAD comparisons with previously reported spectra were 2.7 % and 9.7 % , respectively ( TASMIP ) , 0.1 % and 12.0 % , respectively [ R. Birch and M. Marshall , " Computation of bremsstrahlung x - ray spectra and comparison with spectra measured with a
Ge(
Li ) detector , " Phys . -
pubmed
A close correlation between
copeptin concentrations and serum osmolality existed in 68 healthy controls , with a mean osmotic threshold+-SD of 282 + -4 mOsM / kg
H2O. -
pubmed
Prognostic and predictive value of metabolic
tumor volume on
( 18 ) F - FDG PET /
CT in advanced
biliary tract cancer treated with
gemcitabine /
oxaliplatin with or without
erlotinib . -
pubmed
This study aimed to evaluate the prognostic significance and predictive performance of volume - based parameter of
( 18 ) F - fluorodeoxyglucose (
( 18 ) F - FDG ) positron emission tomography / computed tomography ( PET /
CT ) in
biliary tract cancer (
BTC ) . -
pubmed
The bioaccessibility of
salicylic acid (
SA ) can be effectively modified by incorporating the pharmacological compound directly into polymers such as
poly ( anhydride - esters ) . -
pubmed
After simulated digestion conditions , the bioaccessibility of
SA was observed to be statistically different ( p & lt ; 0.0001 ) in each sample : 55.5 + - 2.0 % for free
SA , 31.2 + - 2.4 % the
SA -
diglycolic acid polymer precursor ( SADG ) , and 21.2 + - 3.1 % for SADG -
P ( polymer ) . -
pubmed
In the quantitative study ,
cancerous tissue with Gleason score at least 3 + 3 was distinguished from
normal tissue in the peripheral zone (
PZ ) with an average AUC of 0.75 ( Gd ) , 0.75 ( Gl ) , 0.70 ( Gamma - Gd ) , 0.68 ( apparent diffusion coefficient ,
ADC ) , and 0.82 (
Gd + Gl +
ADC ) . -
pubmed
The differentiation between
PZ and central gland was modest for
Gd ( p & lt ; 0.07 ) , Gl ( p & lt ; 0.06 ) but not significant for Gamma ( p & lt ; 0.2 ) . -
pubmed
The mRNA expression level of
URAT1 was positively correlated with the concentration of
uric acid but , negatively correlated with the expression level of miR-34a . -
pubmed
INTRODUCTION :
Melatonin , secreted by the pineal gland during the night phase , is a regulator of the biological
clock and sleep tendency . -
pubmed
The potentially beneficial effects of
PRM persisted during the 2 weeks of discontinuation period , consistent with
clock stabilizing effects . -
pubmed
Selected Ion Flow Tube Mass Spectrometry ( SIFT -
MS ) was used to quantify
methanol and other volatile compounds in the headspace of one bacterial and 12 fungal lignin - degrading microbial cultures . -
pubmed
Analysis was done using SIFT -
MS with
H3O (+ ) and
NO (+ ) precursors . -
pubmed
SIFT -
MS can be used to quantify
methanol and other volatile compounds in the headspace of microbial cultures and has the potential to be a rapid , sensitive , non - invasive tool useful in elucidating the mechanisms of lignin degradative pathways . -
pubmed
We evaluated
AMG 416 , a long - acting peptide agonist of the
calcium - sensing receptor , to assess its safety , tolerability , and efficacy and to determine a safe and effective starting dose for subsequent phase 2 studies . -
pubmed
The study was not designed to titrate
AMG 416 dosing to achieve a specific
PTH treatment goal . -
pubmed
RESULTS : Analysis of the primary endpoint showed that treatment with
AMG 416 at 10 mg ( Cohort 2 ) and 5 mg ( Cohort 3 ) for up to 4 weeks resulted in mean 49.4 % and 33.0 % reductions from baseline in
PTH during the efficacy assessment phase , respectively ( p & lt ; 0.05 for both cohorts compared to placebo group within the cohort ) . -
pubmed
Meanwhile ,
PSF intervened in the PID - associated alterations in
TCA cycle ,
sugar metabolism ,
amino acid metabolism , and other uncertain metabolic pathways . -
pubmed
The photoluminescence (
PL ) intensity of
TGA - capped QDs is higher than that of
MPA - capped QDs at same QD concentration because of the surface passivation of
TGA . -
pubmed
To systemically investigate the photodegradation ,
CdTe QDs with various
PL peak wavelengths were dispersed in
phosphate buffered saline (
PBS ) and
Tris - borate -
ethylenediaminetetraacetic acid (
TBE ) buffer solutions . -
pubmed
As for
TGA - capped
CdTe QDs , the results of
PL peak wavelengths in TBE buffer solutions indicated that
S ( 2 - ) released by
TGA attached to
Cd ( 2 + ) and formed
CdS - like clusters layer on the surface of aqueous
CdTe QDs . -
pubmed
Blood
glucose was measured every two weeks , and
insulin and
glucose sensitivity tests were assessed at the end of the follow - up . -
pubmed
All
strains contained Q-10 as the predominant
ubiquinone and
C18 : 1omega7c as the major
fatty acid . -
pubmed
Adenosine deaminases acting on RNA ( ADARs ) are editing enzymes that convert
adenosine to
inosine in duplex RNA , a modification reaction with wide - ranging consequences in RNA function . -
pubmed
A
diketopyrrolopyrrole - based co - polymer (
PDPP -
TAT ) was used as the semiconductor and
poly ( methyl methacrylate ) was used as the gate insulator . -
pubmed
FDG - PET and PET /
CT in
Colorectal Cancer . -
pubmed
This article reviews the current use of
FDG PET /
CT for assessment of
colorectal cancer . -
pubmed
The current place of
FDG PET /
CT is discussed in light of recently published studies . -
pubmed
Gating Modulation of the Tumor - Related
Kv10.1 Channel by
Mibefradil . -
pubmed
Given that
mibefradil ( Mb ) is also known to inhibit
K + channels , we decided to study the interaction between this organic compound and the
tumor - related
Kv10.1 channel . -
pubmed
Genetic polymorphisms of
N - acetyltransferase type 2 (
NAT2 ) cause a trimodal distribution of
INH pharmacokinetics in slow , intermediate , and fast acetylators . -
pubmed
To mechanistically assess the trade - off between toxicity and efficacy , we developed a physiologically based pharmacokinetic ( PBPK ) model describing the
NAT2 - dependent pharmacokinetics of
INH and its metabolites . -
pubmed
Subsequently , we evaluated various
INH dosing regimens in
NAT2 - specific immunocompetent and immune - deficient virtual populations . -
pubmed
When
FFM ,
Vitamin D intake , cBPAQ , pBPAQ , and tBPAQ were included in a stepwise multiple linear regression analysis ,
FFM and pBPAQ were predictors of total hip , accounting for 16 % ( p = 0.024 ) , while
FFM and tBPAQ predicted 14 % of the variance in FN ( p = 0.015 ) . -
pubmed
There was a positive correlation between
Vitamin D intake and
L2 -
L4 ( p = 0.025 ) , but other dietary intakes variables were not significant ( p & gt ; 0.05 ) . -
pubmed
Wide Distribution of Genes for
Tetrahydromethanopterin /
Methanofuran - Linked
C1 Transfer Reactions Argues for Their Presence in the Common Ancestor of Bacteria and Archaea . -
pubmed
Since
d6 - acetone can be used as a starting material , the route developed allows for the facile incorporation of isotopic labels which are required for detecting and quantifying trace amounts via
GC /
MS analysis . -
pubmed
Flavodiiron proteins (
FDPs ) are present in organisms from all domains of life and have been described so far to be involved in the detoxification of
oxygen or
nitric oxide (
NO ) , acting as
O2 and / or NO reductases . -
pubmed
Biochemical and in vivo studies revealed that
FlRd is involved in
NO detoxification as part of the bacterial defense mechanisms against reactive
nitrogen species . -
pubmed
coli
FlRd has a clear preference for
NO as a substrate in vitro , exhibiting a very low reactivity toward
O2 . -
pubmed
The overall tetrameric structure revealed a highly conserved flavodiiron core domain , with a
metallo - beta - lactamase - like domain containing a
diiron center , and a flavodoxin domain with a
flavin mononucleotide cofactor . -
pubmed
The dominant
fatty acids of
strain HQYD1 T were identified as C16 : 0 ,
C18 : 0 and iso - C15 : 0 . -
pubmed
TG and
GSH concentrations , together with
gamma - GCS and
gamma - GT activities , were significantly lower , while
GSSG content and the GRR higher , in the
AR than in the YACR aortas ;
GSSG - Red activity did not differ significantly between the two groups . -
pubmed
Heightened levels of lipid and protein oxidation and
H2O2 occurred in the
AR aortas , indicating age - dependent vascular oxidative stress . -
pubmed
Association of A1538 G and C2437 T single
nucleotide polymorphisms in
heat shock protein-70 genes with
diabetic nephropathy among South Indian population . -
pubmed
We report a case wherein
TTP was successfully treated with repeated plasma exchange ( PE ) and
MPO - ANCA - associated
vasculitis with
corticosteroids . -
pubmed
However , some non - sensitizing irritants , such as
sodium dodecyl sulfate (
SDS ) or
methyl salicylate (
MS ) , have been reported as false - positives by this endpoint . -
pubmed
In search of a potential endpoint to enhance the specificity of existing endpoints , we evaluated 3 contact sensitizers ; (
hexyl cinnamic aldehyde [
HCA ] ,
oxazolone [
OXA ] , and
2,4 - dinitrochlorobenzene [
DNCB ] ) , 1 respiratory sensitizer (
toluene 2,4 - diisocyanate [
TDI ] ) , and 2 non - sensitizing irritants (
MS and
SDS ) by several endpoints in LLNA . -
pubmed
Cells transfected with
TIRAP ,
IRAK1 ,
IRAK4 or
TAK1 all showed a decrease in
fsl-1 - induced expression of
cytokines (
IL-1alpha ,
IL-1beta ,
IL-6 ) ,
chemokines (
GRO - alpha ,
IL-8 ,
MCP-1 ) ,
adhesion molecule ICAM-1 ,
cyclooxygenase ( COX )- 2 mRNA and release of
PGF2alpha and
MMP-9 expression . -
pubmed
The proliferation , and migration and invasion capacities of prostate cells were determined by
EdU and Transwell assays following transfection with
BLM - targeting short hairpin RNA ( shRNA ) . -
pubmed
,
gefitinib in the maintenance setting irrespective of
EGFR status . -
pubmed
OBJECTIVE : The aim was to evaluate the effect of short glass - fiber / filler particles proportion on fracture toughness ( FT ) and flexural strength (
FS ) of an experimental flowable fiber - reinforced composite ( Exp - SFRC ) with two
methacrylate resin formulations . -
pubmed
While
sugar sweetened beverage (
SSB ) consumption has declined in the last 15 years , consumption of SSBs is still high among children and adolescents . -
pubmed
Herein , we demonstrate the 1 T '
transition metal telluride atomic layers (
WTe2 and
MoTe2 ) as ultrasensitive platforms for CM - based
SERS . -
pubmed
To circumvent these obstacles , we anchored
CsPbBr3 QDs (
CPB ) on
NHx -rich porous g -
C3 N4 nanosheets (
PCN ) to construct the composite photocatalysts via
N - Br chemical bonding . -
pubmed
The obtained results demonstrated that
fipronil may undergo a clearance by the liver and it is exclusively metabolized by the
CYP3A4 isoform . -
pubmed
To obtain new insight into parasite
sphingolipid catabolism , the substrate specificity of
TbnSMase was investigated using electrospray ionization tandem mass spectrometry ( ESI - MS / MS ) . -
pubmed
Recombinant
TbnSMase was shown to degrade
sphingomyelin ,
inositol - phosphoceramide and
ethanolamine -
phosphoceramide sphingolipid substrates , consistent with the
sphingolipid complement of the parasites . -
pubmed
TbnSMase also catabolized
ceramide-1 - phosphate , but was inactive towards
sphingosine-1 - phosphate . -
pubmed
The broad - range specificity of this enzyme towards
sphingolipid species is a unique feature of
TbnSMase . -
pubmed
Additionally , ESI -
MS /
MS analysis revealed previously uncharacterized activity towards
lyso - phosphatidylcholine despite the enzyme 's inability to degrade
phosphatidylcholine . -
pubmed
AIM : The secretolytic drug
ambroxol may be useful for the treatment of
neuropathic pain due to its multiple modes of action . -
pubmed
Calcineurin inhibitors ,
cyclosporine and
tacrolimus , and
everolimus are used to prevent reactions following organ transplantation . -
pubmed
Quantitative real - time
polymerase chain reaction results confirmed the alterations in the expression levels of genes encoding components of pathways involved in
phenylpropanoids and lipids . -
pubmed
Moreover ,
PFB@
PLLA loads high amount of
oxygen and US - triggering
PFB@
PLLA reoxygenation effectively inhibits the expression of
hypoxia - related proteins (
HIF-1alpha and
CAIX ) , reduces
lactate secretion and glycolysis , which modulates hypoxic microenvironment and inhibits
cancer cell migration and invasion in vitro . -
pubmed
Sodium dichloroacetate (
DCA ) is a mitochondrial
pyruvate dehydrogenase kinase inhibitor , and has been shown to display vasoprotective effects in chronic
ischemic stroke . -
pubmed
N 4 - Hydroxycytidine (
NHC ) is an antiviral
ribonucleoside analog that acts as a competitive alternative substrate for virally encoded
RNA - dependent RNA polymerases . -
pubmed
The
mitochondrial DNA - dependent RNA polymerase (
POLRMT ) has been shown to incorporate some
nucleotide analogs into mitochondrial RNAs , resulting in substantial
mitochondrial toxicity . -
pubmed
We demonstrate that the
5 '- triphosphate of
NHC can be used by
POLRMT for incorporation into nascent RNA chain but does not cause immediate chain termination . -
pubmed
HbII and
HbIII contain
B10 tyrosine (
Tyr ) and E7
glutamine (
Gln ) in the
heme pocket generating an efficient
hydrogen bonding network with the ( HbII - HbIII)-
O2 species , leading to very low ligand dissociation rates . -
pubmed
The results suggest that acidic pH generates conformational changes associated with
B10 and E7
heme pocket
amino acids , weakening the ( HbII - HbIII)-
O2 hydrogen bond network . -
pubmed
The
sodium / iodide symporter (
NIS ) has positive expression in
thyroid carcinomas with good prognoses and plays a critical role in
radioiodine therapy response . -
pubmed
Peroxisome proliferator - activated receptor gamma (
PPARgamma ) agonists and
retinoids are two types of redifferentiating agents . -
pubmed
Moreover ,
rosiglitazone treatment reduced cell growth and increased
NIS protein expression in
thyroid cancer cells under normoxic or hypoxic conditions . -
pubmed
In addition ,
bexarotene potentiated the effects of
rosiglitazone on cell growth and
NIS protein expression . -
pubmed
Here , we present a step wise protocol to ( a ) measure
Ca2 + interaction with the
Ca2 + -sensor
guanylate cyclase - activating protein 1 , ( b ) to design an ITC experiment and prepare samples , ( c ) to remove
Ca2 + nearly completely from
Ca2 + binding proteins without using a chelating agent like
EGTA . -
pubmed
Haemoglobin DIbadan ( beta 87
threonine leads to
lysine ) was originally found in Ibadan , an area with a high incidence of
sickle cell haemoglobin (
HbS ) . -
pubmed
However , the
EDTA test showed absent or low responses in 10 of 11 cases studied with
GP 62 . -
pubmed
In
chronic renal failure normal responses can be observed despite an abnormal basal
PTH level , since it is falsely elevated by the accumulation of
COOH - terminal fragments . -
pubmed
Additional studies exhibited
post - transcriptional regulation by
phorbol ester . -
pubmed
DMSO was also shown to regulate
c - myb at the
post - transcriptional level . -
pubmed
Interestingly , the
post - transcriptional regulation of c - myb by
DMSO required continuous transcription . -
pubmed
To determine whether
aromatase activity of cultured granulosa cells obtained from relatively low
estradiol (
E2 ) responders ( serum
E2 & lt ; 1000 pg / ml ) to
hMG therapy differed from that of good responders (
E2 & gt ; or = 1000 pg / ml ) , we prospectively compared serum E2 on the day of
human chorionic gonadotropin administration to in vitro
aromatase activity following a 72 - h culture . -
pubmed
Benzodiazepine receptor ligands and
hepatic encephalopathy : further unfolding of the
GABA story . -
pubmed
Addition of 0.5 mumol / l
MCLA and a stimulatory agent , such as
N - formyl - methionyl - leucyl - phenylalanine (
fMLP ) and
phorbol myristate acetate (
PMA ) , to a suspension of the cells treated with
gamma interferon (
IFN gamma ) caused a marked luminescence which was inhibited by 0.5 mumol / l
superoxide dismutase (
SOD ) . -
pubmed
On the other hand , the cells which were not treated with
IFN gamma caused no significant luminescence by the stimulation with either
fMLP or
PMA . -
pubmed
Surprisingly ,
rCGCUUGCG forms a very stable duplex at pH 7 in 1 M
NaCl with a
TM of 44.8 degrees C at 1 x 10(-4 ) M and a delta G degrees 37 of -7.2 kcal / mol . -
pubmed
At neutral pH ,
rCGCCCGCG is one of the least stable duplexes with a
TM of 33.2 degrees C and delta G degrees 37 of -5.1 kcal / mol . -
pubmed
rCGCCAGCG and
rGGCACGCC have a small pH dependence , with
TM increases of 5 and 3 degrees C , respectively , upon lowering the pH from 7 to 5.5 . -
pubmed
rCGCCUGCG and
rCGCUCGCG also show little pH dependence , with
TM increases of 0.8 and 1.4 degrees C , respectively , upon lowering the pH to 5.5. -
pubmed
Antibodies raised against purified
P-450IIIA inhibited the microsomal formation of quantitatively minor
cannabinoid metabolites but had no effect on the major metabolites . -
pubmed
Unlike anti -
P-450IIIA antibody , antibody raised against purified
P-450THC markedly inhibited the microsomal formation of all major
cannabinoid metabolites . -
pubmed
The significance of its
beta 1 - adrenoceptor selectivity was examined at various cardiovascular activation levels established by either incremental
isoprenaline infusion or graded treadmill exercise . -
pubmed
Characteristically of a
beta 1 - selective agent ,
esmolol had only a slight effect on the
isoprenaline - induced
reduction in diastolic blood pressure . -
pubmed
beta - Adrenoceptor blockade was virtually reversed within 20 min of discontinuation of
esmolol infusion . -
pubmed
The ranking order was
RRR - alpha - T greater than
RRR - beta - T greater than
RRR - gamma - T greater than or equal to
R - alpha - TT greater than RRE - delta - T. Accordingly , the biopotency of a
palm - oil residue was assessed and expressed as
alpha - tocopherol equivalents ( alpha - TEs ) . -
pubmed
The release of
pyruvate kinase , a variable in the nutrition - linked necrotizing
myopathy , into the plasma was dose - dependently inhibited by the
RRR - alpha - T standard and the corresponding
alpha - TE from this residue . -
pubmed
Prostacyclin synthesis from aorta segments induced by
thrombin or
ionomycin was higher than the spontaneous release . -
pubmed
However , there was no difference between the depleted group and groups treated with
RRR - alpha - T or
alpha - TEs from the
palm - oil residue . -
pubmed
Upon supplementation with
RRR - alpha - T or alpha - TEs from the
palm - oil residue , reduced
IgG concentrations were observed , similar to those of animals on a commercial diet containing adequate amounts of
vitamin E. -
pubmed
Before entry they circumvent the lytic activity of the complement cascade , during cell entry they avoid being killed by toxic
oxygen metabolites and after entry they escape nonoxidative killing mechanisms such as degradation by
lysosomal hydrolases . -
pubmed
PAG -
DRP with properties of spatial and temporal summation propagates decrementally along the dorsal root and is inhibited by
picrotoxin . -
pubmed
In addition , the possibility that colonic
glucuronyltransferase might be involved in the biotransformation of
MAM was also investigated . -
pubmed
MAM inhibition of colonic
4 - methylumbelliferone glucuronyltransferase was non - competitive , suggesting indirectly that colonic
glucuronyltransferase is not involved in conjugation of
MAM . -
pubmed
Temporal relationships among fetal urine flow ,
ANF , and
AVP responses to hypertonic infusions . -
pubmed
The fetal urine flow response to acute increases in osmolality may be mediated by changes in the plasma concentrations of
atrial natriuretic factor (
ANF ) ,
arginine vasopressin (
AVP ) , and/or
angiotensin II (
ANG II ) . -
pubmed
Thus the initial increase in fetal urine flow in response to acute hypertonic infusions is temporally related to a rise in fetal plasma
ANF , whereas the subsequent fall in urine flow is temporally related to a fall in plasma
ANF and a simultaneous rise in
AVP concentration . -
pubmed
This suggests that
ANF may contribute to the acute urine flow increase after hypertonic infusion , whereas
AVP appears to be more important for the long - term regulation of fetal urine flow . -
pubmed
coli dnaK gene product , the
hsp70 homolog , can reactivate heat - inactivated
RNA polymerase in an
ATP hydrolysis - dependent manner . -
pubmed
coli
hsp70 homolog , the
dnaK gene product , protects the host
RNA polymerase enzyme from heat inactivation in an
ATP - independent reaction . -
pubmed
In addition , we show that heat - inactivated and aggregated
RNA polymerase is both disaggregated and reactivated following simultaneous incubation with
DnaK protein and hydrolyzable
ATP . -
pubmed
This study was aimed at evaluating the effect of
diltiazem , a
calcium channel blocking agent , on
myotonia induced by
2,4 - dichlorophenoxyacetic acid (
2,4 - D ) . -
pubmed
Influence of
D - glucose upon the respiratory and secretory response of
insulin - producing
tumor cells to
2 - aminobicyclo [ 2,2,1 ] heptane-2 - carboxylic acid . -
pubmed
The metabolic and secretory effects of
2 - aminobicyclo [ 2,2,1 ] heptane-2 - carboxylic acid are opposed to those of
D - glucose , which increases respiration ,
ATP content , and
insulin release , while lowering
NH4 + production and
14CO2 output from the prelabeled cells . -
pubmed
These findings suggest that , in
tumor as in normal islet cells , the regulation of
insulin release by exogenous nutrients depends on the availability of endogenous
ATP . -
pubmed
Atrial concentration of
i alpha - rANP was significantly lower in the
DOCA - salt group than in the other groups at 1 week , and was reduced in the
DOCA and
DOCA - salt groups as compared to the control group at 8 weeks . -
pubmed
On the other hand , plasma concentration of
i alpha - rANP was significantly higher in the
DOCA and the
DOCA - salt groups than in the control group at 1 week ; the
DOCA and
DOCA - salt group values were also higher than the control and salt group values at 8 weeks . -
pubmed
In the present study , fragments of proliferative and secretory phase endometrium were cultured in media in the presence and absence of
progesterone ( P ) and
17 beta - estradiol (
E2 ) for 96 h. The
PP12 concentrations in the media and tissues were measured by RIA , and de novo synthesis was investigated by measuring the incorporation of
[ 35S ] methionine into
PP12 . -
pubmed
All secretory endometria released
PP12 into the medium in the presence and absence of added
P and E2 . -
pubmed
The enthalpy of dilution of
alpha - chymotrypsin and of
proflavin , and the enthalpy of binding of
indole ,
N - acetyl - D - tryptophan , and
proflavin to
alpha - chymotrypsin . -
pubmed
The findings suggest that the prejunctional inhibitory effect of
methysergide may be mediated via stimulation of a
5 - HT receptor which , unlike the
D - receptor , is not blocked by
cyproheptadine . -
pubmed
In dispersed mucosal cells from guinea - pig stomach ,
somatostatin inhibited in a noncompetitive fashion ( Ki , 2 x 10(-8 ) M ) the increase in cellular
cyclic AMP caused by
histamine but not by
prostaglandin E1 or
phosphodiesterase inhibitors . -
pubmed
Neoplastic brain levels of the
Photofrin II isotope were higher than in the
HpD studies , and highly fluorescent . -
pubmed
Epidermal growth factor stimulates
tyrosine phosphorylation of the
myosin regulatory light chain from smooth muscle . -
pubmed
Two moles of
phosphate are incorporated into
tyrosine per mol of
LC20 . -
pubmed
Tyrosine 142 is located within a sequence similar to that of autophosphorylated
tyrosine kinases in that five of the seven
amino acids on the NH2 - terminal side are
acidic . -
pubmed
The possibility that a
lysine - rich protein was involved in the acute stimulation of steroidogenesis by
ACTH was investigated using [
3H ] labelled
lysine and isolated adrenal cells . -
pubmed
However cells incubated in a
lysine - free medium showed the same response to
ACTH as cells incubated in a
lysine - containing medium . -
pubmed
It was also demonstrated that
ACTH had no effect on the incorporation of tritiated
lysine into the protein or small peptide fractions . -
pubmed
These observations suggest that a rapidly synthesised ,
lysine - rich protein is not involved in the acute response to
ACTH . -
pubmed
The influence of
oxygen donor ligation on the spectroscopic properties of
ferric cytochrome P-450 :
ester ,
ether and
ketone co - ordination to the haem
iron . -
pubmed
Homogeneous low - spin complexes of
cytochrome P-450 - CAM with
esters ,
ethers and
ketones have been prepared and characterized by u.v.-visible absorption , circular dichroism (
CD ) , magnetic circular dichroism (
MCD ) and electron paramagnetic resonance (
EPR ) spectroscopy . -
pubmed
Anomalous spectral and substrate binding properties have been reported in the study of
cytochrome P-450 under conditions employing solvents and non -
phosphate buffers containing
oxygen functionalities , and have been attributed to ' solvent effects ' . -
pubmed
The present work , in combination with our previous report of
alcohol ,
amide and
carboxylate oxygen donor complexes of
cytochrome P-450 , is evidence that a wide variety of
oxygen - donor species are capable of direct ligation to the
haem iron of
cytochrome P-450 . -
pubmed
This leads us to suggest
oxygen - donor ligation to
cytochrome P-450 as the origin of spectral and substrate binding anomalies previously attributed to solvent effects . -
pubmed
The T lymphocyte - oriented mitogens concanavalin A and
phytohemagglutinin and the B lymphocyte - oriented mitogen lipopolysaccharide were used to study variations in
[ 3H ] thymidine incorporation in lymphocytes cultured in media deficient in one mineral element . -
pubmed
To assess effects of industrial and environmental pollution on analytical characteristics of humic substances , we isolated
humic acids ( HA 's ) and
fulvic acids (
FA 's ) from unpolluted and polluted soils and sediments . -
pubmed
Following purification , the HA 's and
FA 's were characterized by elemental
( C , H , O , N , S ) and functional group ( CO2H , phenolic OH , total acidity ) analyses , infrared ( IR ) spectrophotometry , differential thermal analysis ( DTA ) and by metal ( Fe , Al , Cu , Mn , Pb , Ni , Co , Zn , Cr , Cd , Hg , Ca and Mg ) analyses . -
pubmed
The effect of
L - tryptophan on motor activity and its prevention by an
extracerebral decarboxylase inhibitor and by
5 - HT receptor blockers . -
pubmed
A further indication that the behavioural effect of
tryptophan was not due to increased brain
5 - HT was its prevention by
R04 - 4602 at a dose sufficient to block peripheral but not central
L - aromatic amino acid decarboxylase . -
pubmed
The adsorbed
von Willebrand factor can be eluted from fibrillar
collagen with 1 M
NaCl . -
pubmed
Heparin also inhibited
platelet aggregation , release of
( 14C ) 5 - HT and production of
malondialdehyde in response to
collagen and
thrombin . -
pubmed
The results indicate that in addition to the effects of heparin on platelet function mediated by anti -
thrombin activity and the previously described augmentation of responses to
ADP , heparin has weak inhibitory activity against platelet -
collagen interactions . -
pubmed
NOS activity detected by
[ 3H ] arginine to
[ 3H ] citrulline conversion rose 109 % . -
pubmed
The increase in
NOS - III expression does not explain the declines in pulmonary endothelial
NO production previously observed following prolonged
hypoxia in this model . -
pubmed
Alternatively , the fall in
NO production may be related to diminished
NOS co - factor availability . -
pubmed
Relaxation to
A-23187 , but not
ADP , was abolished by removal of the endothelium or exposure to 100 microM
NO2 - L - arginine.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
Moreover , a pronounced increase in
ALA - B concentrations occurred when the inhibition rate of erythrocyte
ALA dehydratase exceeded 85 % . -
pubmed
A long
biotin ester provided more effective binding of the
B - RBC to immobilized
avidin and greater lysis by complement , than a short
biotin ester . -
pubmed
The mechanisms involved in such effects were evaluated by measuring pituitary
GH mRNA levels and hypothalamic levels of
GH - releasing hormone (
GHRH ) and
somatostatin ( SRIF ) mRNAs . -
pubmed
in pups of either sex , from birth until 10 days of age , caused a significant increase in baseline plasma
GH and potentiated the
GH response to the
[ Met5 ]- enkephalin analog
FK 33 - 824 ( 1 mg / kg , s.c . ) -
pubmed
, 3 h before ) did not change significantly basal
GH levels but potentiated
FK 33 - 824 ( 0.3 mg / kg , intracarotid)-induced stimulation of
GH secretion . -
pubmed
, for 3 days ) failed to modify basal and
FK 33 - 824 - induced
GH secretion , caused a significant reduction in hypothalamic
GHRH mRNA levels and left
SRIF and
GH mRNAs unchanged . -
pubmed
[ Effectiveness and tolerability of oral liquid
ferrous gluconate in
iron - deficiency anemia in pregnancy and in the immediate
post - partum period : comparison with other liquid or solid formulations containing bivalent or trivalent
iron ] . -
pubmed
It is also now clear that women who take
iron supplements during pregnancy do not suffer the same
post - natal reduction in
hemoglobin and
ferritin as those who do n't make it . -
pubmed
RESULTS : Analysis of the therapeutic efficacy parameters ( red blood cells ,
hemoglobin , hematocrit and serum
iron ) showed significant improvements but no statistically significant differences between the groups . -
pubmed
This result supports the hypothesis that
DME , together with
pyruvate dehydrogenase , forms a pathway in which
malate is converted to
acetyl - CoA. -
pubmed
Interaction of
bromocresol green with different
serum albumins studied by fluorescence quenching . -
pubmed
The binding of
bromocresol green to
bovine serum albumin at micromolar concentrations leads to quenching of
protein fluorescence . -
pubmed
This property has been used here to study interaction of
bromocresol green with
bovine serum albumin as a function of pH and ionic strength . -
pubmed
The affinity of
bromocresol green for
bovine serum albumin remains virtually unchanged between pH 4.0 and 8.0 but decreases by about 7 fold with increase in ionic strength from 0.01 to 1.0 . -
pubmed
Although all the
albumins studied bind
bromocresol green , they show considerable differences in their affinities towards the dye . -
pubmed
However , there was no correlation between the
non - GP GBM IgM antibody levels and the decrease in
GFR as measured by
Cr EDTA clearance . -
pubmed
Gonadal
steroids are known to alter
GH secretion as well as tissue metabolism . -
pubmed
The present study was designed to examine the effects of short term ( 2- to 3 - week ) alterations in gonadal
steroids on basal pulsatile ( nonstimulated ) and exercise- and
GH - releasing hormone - stimulated
GH secretion , urinary
nitrogen excretion , and basal and exercise - stimulated
oxygen consumption . -
pubmed
Raising
testosterone to supraphysiological levels increased pulsatile
GH secretion by 62 % over that with
leuprolide and by 22 % over that with saline ( P & lt ; 0.05 ) . -
pubmed
High dose
testosterone treatment also increased serum
insulin - like growth factor I concentrations by 21 % and 34 % over those during the eugonadal and hypogonadal states , respectively ( P & lt ; 0.01 ) . -
pubmed
Testosterone did not affect either exercise- or
GRF - stimulated
GH secretion . -
pubmed
In protocol 2 ,
stanozolol did not affect any parameter of
GH secretion . -
pubmed
To examine the interaction between
GH secretion and
testosterone on urinary
nitrogen excretion and basal metabolic rate , a one - way analysis of covariance was undertaken . -
pubmed
Statistical examination of
GH production as the covariate and
testosterone ( by tertile ) as the interactive factor demonstrated significant relationships between serum
testosterone levels and either urinary
nitrogen ( P & lt ; 0.02 ) or basal metabolic rate ( P & lt ; 0.01 ) , but not
GH secretion ( P =
NS ) . -
pubmed
In summary , these results demonstrate that short term modulation of the
androgen milieu affects metabolic outcome without necessitating changes in
GH secretion . -
pubmed
We also measured the
glucose and
insulin response to an oral
glucose tolerance test and the
insulin sensitivity ( [ SI ] by modified minimal model analysis ) . -
pubmed
Measures of overall
obesity ( BMI ) , total fat [ TF ] , and/or SCF measured at the abdomen by MRI ) ,
glucose /
insulin metabolism and
SI , and central fat patterning ( WHR or IAF measured by MRI ) were correlated with mean arterial pressure ( MAP ) ,
triglyceride (
TG ) , and
high - density lipoprotein cholesterol (
HDL - C ) levels in univariate analysis and after controlling for age and gender . -
pubmed
No index of overall
obesity , fat patterning ,
glucose /
insulin metabolism , and/or
SI , was independently related to the plasma concentration of
HDL - C after controlling for any one of the other two . -
pubmed
Direct measurement of
glucose /
insulin metabolism and
SI , as well as fat patterning , provides information on their relative associations with
CVD risk factors . -
pubmed
The measures of
glucose /
insulin metabolism and
SI were more consistently related to
dyslipidemia and
hypertension than were the overall
obesity and fat patterning in this healthy population . -
pubmed
These conversions were purely biocatalytic , since in acidified water ( pH & lt ; 3.5 ) almost 50 %
linalool was recovered unchanged , the
rest was lost by evaporation . -
pubmed
It was therefore hypothesized that expression of
MgtA and
MgtCB is increased upon invasion of eukaryotic cells because of a lack of intravacuolar
Mg2 + . -
pubmed
typhimurium was grown at pH 5.2 , the capacity of the constitutive
CorA transporter in mediating
Mg2 + was greater than that at pH 7.4 . -
pubmed
typhimurium could obtain sufficient
Mg2 + via the
CorA system . -
pubmed
Compared to induction at pH 7.4 , exposure to pH 5.2 almost completely abolished induction of
mgtA at low
Mg2 + concentrations but diminished induction of
mgtCB only twofold . -
pubmed
Adaptation of cells to acid pH by overnight growth resulted in normal levels of induction of
mgtA and
mgtCB at low
Mg2 + concentrations . -
pubmed
Conversely , repression of
mgtA and
mgtCB expression by increased extracellular
Mg2 + was relatively insensitive to acid . -
pubmed
After adjustment for age , sex ,
thallium perfusion defects , and other confounders , failure to reach 85 % of the age - predicted maximum heart rate was associated with increased risk of
death ( adjusted relative risk [ RR ] , 1.84 ; 95 % confidence interval [ CI ] , 1.13 - 3.00 ; P=.01 ) , as was a low chronotropic index ( adjusted RR ,
2.19 ; 95 % CI , 1.43 - 3.44 ; P<.001 ) . -
pubmed
RESULTS : In the open trial , after 4 weeks ' treatment with
HpPro 7.5 mg orally three times daily , acute
hepatitis ,
chronic hepatitis and
fatty liver cases showed rapid decrease of
SGOT and
SGPT . -
pubmed
Low - dose
fosinopril led to 70 % reduction in urinary
albumin excretion ( UAE ) ( 220 + /- 199 vs 82 + /- 67 mg/24 - h , p & lt ; 0.05 ) , but UAE did not change during placebo treatment ; the maximal change became apparent after 3 months and was reversible once treatment was stopped . -
pubmed
The
EXT1 and
EXT2 genes encode glycosyltransferases involved in biosynthesis of heparan
sulphate proteoglycans . -
pubmed
The purpose of the present work is to further modify the previously developed headspace ( HS )
GC -
MS method for blood
isoflurane determination to analyze and distinguish five common clinical inhalation anesthetics , simultaneously . -
pubmed
, 0.25 microm film thickness DB-5 capillary column along with an adequate
GC temperature program , which gives the five inhalation anesthetics , including
isoflurane and its isomer ,
enflurane , a high resolution . -
pubmed
Importance of timing of
post - contrast MRI in
rheumatoid arthritis : what happens during the first 60 minutes after
IV gadolinium - DTPA ? -
pubmed
OBJECTIVE : To determine the time period after
IV MRI contrast (
gadolinium - DTPA (
Gd ) ) injection in which synovial membrane volume determination is reliable . -
pubmed
METHODS : MRI of five RA knees with clinical
synovitis was carried out , with axial , T(1 ) weighted , spin echo images before
IV Gd injection and every 1.75 minutes for 60 minutes
post - Gd . -
pubmed
Compared with volumes at all other time points , synovial membrane volumes at 0.75 and 2.50 minutes
post -
Gd were significantly lower ( Wilcoxon - Pratt ) , suggesting that some synovial membrane areas had not yet exceeded the enhancement threshold . -
pubmed
Mutant
acyl - coenzyme A : cholesterol acyltransferase 1 devoid of
cysteine residues remains catalytically active . -
pubmed
Acyl - coenzyme A : cholesterol acyltransferase (
ACAT ) plays important roles in cellular
cholesterol homeostasis and in the early stages of
atherosclerosis . -
pubmed
We constructed various
ACAT1 mutants that contained either single
cysteine to
alanine substitution at various positions , contained a reduced number of
cysteines , or contained no
cysteine at all . -
pubmed
Therefore ,
cysteine is not essential for
ACAT catalysis . -
pubmed
The availability of the
cysteine - free
ACAT1 will facilitate future
ACAT structure function studies . -
pubmed
Additional studies show that
Cys467 is one of the major target sites that leads to
p - chloromercuribenzene sulfonic acid - mediated
ACAT1 inactivation , suggesting that
Cys467 may be near the
ACAT active site(s ) . -
pubmed
Male fertility is compatible with an
Arg(840)
Cys substitution in the
AR in a large Chinese family affected with divergent phenotypes of
AR insensitivity syndrome . -
pubmed
We reported here an
Arg(840)
Cys substitution in the
AR gene in a large Chinese pedigree affected with
AIS . -
pubmed
We now identify a second site in
A1 at
Lys(36 ) that is cleaved by
factor Xa . -
pubmed
These affinity values were consistent with values obtained by fluorescence energy transfer using
acrylodan - labeled A2 and
fluorescein - labeled
A1 . -
pubmed
This resulted in part from a 5 - fold increase in K(m ) for
factor X when
A1 was cleaved at
Arg(336 ) . -
pubmed
Among the enzymes that regulate
dopamine turnover ,
tyrosine hydroxylase (
TH ) , the rate - limiting anabolic enzyme , could be a target for endocrine feedback regulation . -
pubmed
We demonstrated that
preoptic TH and
estradiol receptor mRNA levels are greatly decreased by gonadectomy during vitellogenesis . -
pubmed
For
TH expression , this effect was reversed in part by
estradiol supplementation . -
pubmed
We have also confirmed the existence of an inhibitory gonadal feedback on
FSH secretion , mediated by
estradiol . -
pubmed
The stimulating effect of
estradiol on
TH expression found in this study could be a pathway involved in gonadal feedback on
gonadotropin release . -
pubmed
Analysis of
7 - HPG in DNA by gas chromatography - high - resolution mass spectrometry (
GC - HRMS ) showed a linear response in
7 - HPG for all three tissues after 3 days of exposure , and for NRE and lung after 20 days of exposure . -
pubmed
PURPOSE : To study the kinetics of
polylactide (
PLA ) nanoparticle (
NP ) localization within the intraocular tissues and to evaluate their potential to release encapsulated material . -
pubmed
METHODS : A single intravitreous injection ( 5 micro L ) of an
NP suspension ( 2.2 mg / mL ) encapsulating either
Rh-6 G ( Rh ) or
Nile red ( Nr ) was performed . -
pubmed
PLA NPs were still present within the
RPE cells 4 months after a single intravitreous injection . -
pubmed
CONCLUSIONS : Intravitreous injection of
PLA NPs appears to result in transretinal movement , with a preferential localization in the
RPE cells . -
pubmed
The inhibition of
porphobilinogen deaminase by
isoporphobilinogen . -
pubmed
Subjects with a shortened activated partial
thromboplastin time show increased in - hospital mortality associated with elevated D - dimer ,
C - reactive protein and
glucose levels . -
pubmed
D - dimer ,
C - reactive protein (
CRP ) and
glucose , markers that have been related with increased mortality , were tested . -
pubmed
Non - survivors with short aPTTs had significantly higher plasma D - dimer ,
CRP and
glucose levels compared with survivors . -
pubmed
By using truncated versions of
HRGP , we demonstrate that the isolated 150
amino acid - residue
His /
Pro - rich domain , which is also released by spontaneous proteolysis from purified
HRGP , mediates the inhibitory effect on chemotaxis . -
pubmed
Moreover , the
His /
Pro - rich domain must be released from
HRGP to exert its effect . -
pubmed
Our working hypotheses were : ( 1 ) liver expresses considerable
SSADH activity and therefore represents a major source of
GHB output , ( 2 ) correction of
liver enzyme deficiency will reduce
GHB load both peripherally and in the central nervous system , and ( 3 )
SSADH expression will improve survival . -
pubmed
Molecular aging of
tau :
disulfide - independent aggregation and non - enzymatic degradation in vitro and in vivo . -
pubmed
Most importantly ,
tau was spontaneously degraded , generating fragments that start from bulky residues next to
asparaginyl residues . -
pubmed
This spontaneous degradation of
tau probably represents non - enzymatic cleavage through the formation of
succinimide intermediates . -
pubmed
Similar degradation products starting from the bulky residues next to
asparaginyl residues were found in the smeared
tau in vivo partially purified from the homogenates from
Alzheimer ' s disease brains . -
pubmed
Inhibition with
merthiolate of the
mucooligosaccharase fraction of testis
hyaluronidase (
mesomucinase ) . -
pubmed
Interestingly , among all the studied
P450 enzymes , only
CYP2D1 was not affected by
NO donors . -
pubmed
In the present study , we systematically evaluated the effects of
NO on the
enzyme kinetic parameters of marker substrates for a range of
P450 enzymes , including
2D1 . -
pubmed
These results indicate that
NO can potentially change both the Vmax and Km of various substrates selectively and confirm our previous findings that the activity of
CYP2D1 is not affected by
NO donors . -
pubmed
Adrenalectomy per se had no effect on the concentration of
LH ,
FSH , or
testosterone or on the weights of the prostate or seminal vesicles . -
pubmed
AIM : To investigate the release of
cyclodextrin-5 - aminosalicylic acid (
CyD-5 - ASA ) in cecum and colon . -
pubmed
The observation that the Q(e ) peak precedes the Q(q ) decline would be consistent with the decreases in Calvin cycle
carbon flow occurring at the
kinase reactions prior to
glyceraldehyde-3 - phosphate dehydrogenase . -
pubmed
The isolation of
asparagine from an enzymic digest of
edestin . -
pubmed
Platelet function was measured immediately at admission , and
aspirin resistance was defined as a
collagen /
epinephrine Closure Time ( CT(CEPI))<165 s. RESULTS : We found that 70 ( 23.5 % ) patients were
aspirin resistant , and 70 ( 23.5 % ) patients ended up with the diagnosis of an
AMI . -
pubmed
The most common types of barrier membranes used for bone or tissue regeneration are made of expanded -
polytetrafluoroethylene ( e -
PTFE ) or resorbable materials , such as
collagen . -
pubmed
Calcium - sensitive regulation of
monoamine oxidase - A contributes to the production of
peroxyradicals in hippocampal cultures : implications for
Alzheimer disease - related pathology . -
pubmed
BACKGROUND :
Calcium (
Ca2 + ) has recently been shown to selectively increase the activity of
monoamine oxidase - A (
MAO - A ) , a mitochondria - bound enzyme that generates
peroxyradicals as a natural by - product of the deamination of neurotransmitters such as
serotonin . -
pubmed
It has also been suggested that increased intracellular free
Ca2 + levels as well as
MAO - A may be contributing to the oxidative stress associated with
Alzheimer disease (
AD ) . -
pubmed
The effects of
A23187 and
CB-28 K are both independent of any change in
MAO - A protein or gene expression . -
pubmed
CONCLUSION : These data suggest that increases in intracellular
Ca2 + availability could contribute to a
MAO - A - mediated mechanism with a role in
AD - related oxidative stress . -
pubmed
On the other hand , hepatic
CYP7A1 activity , fecal dry weight , and fecal excretion of
bile acids increased linearly with increasing DF , while body
weight gain decreased . -
pubmed
The complex is insensitive to acute
rapamycin exposure and has shown functions in controlling cell growth and
actin cytoskeletal assembly . -
pubmed
It has been suggested that
dimethylsulfoxide (
DMSO ) acts via the
oxytocin / oxytocin receptor pathway because an
oxytocin receptor antagonist not only blocks
oxytocin - induced cardiomyocyte differentiation , but also blocks
DMSO - induced differentiation . -
pubmed
Monoterpene cineole ,
pentacyclic triterpenes and
steroids were identified by TLC and
GC - FID . -
pubmed
These findings now place
PKCtheta as a key signaling
molecule in the positive selection of thymocytes as well as in the activation of mature T cells . -
pubmed
Mutants of
hsPrP(84 - 114 ) with two or one
histidyl residues have also been synthesized and their
copper ( II ) complexes studied by CD spectroscopy . -
pubmed
Each individual contiguous network of
Ni ,
YSZ , and porosity was identified and labeled according to whether it was contiguous with the
rest of the electrode . -
pubmed
In
SSc , the presence of
anti - S cerevisiae alpha - enolase antibodies was associated with
interstitial lung disease (
ILD ) , decreased total lung capacity ( 73.2 % vs 89.7 % ; p<0.001 ) and diffusion capacity for
carbon monoxide ( 47.4 % vs 62.3 % ; p<0.001 ) , and
antitopoisomerase 1 antibodies ( 46 % vs 21 % ; p = 0.005 ) but not anticentromere antibodies ( 11 % vs 34 % ; p = 0.006 ) . -
pubmed
Here , we focus on the origin and migratory routs of prospective CB(1)/
cholecystokinin ( CCK)+ and
CB(1)/
reelin /
calretinin+
gamma - aminobutyric acid (
GABA)-ergic hippocampal interneurons . -
pubmed
METHODS : We prepared cationic
NP encapsulated with a fluorescence marker ( FITC ) by emulsion solvent diffusion method , succeeded to formulate an
NP - eluting stent with a novel cation electrodeposition coating technology , and compared the in vitro and in vivo characteristics of the
FITC - loaded NP - eluting stent with dip - coated
FITC - eluting stent and bare metal stent . -
pubmed
These criteria are evaluated with respect to the confirmation of
cocaine and its metabolites by single quadrupole liquid chromatography / mass spectrometry ( LC /
MS ) and problems are highlighted . -
pubmed
Spiked samples , proficiency testing samples , certified reference materials and samples from real cases that had screened positive for
cocaine derivatives by immunoassay were subjected to confirmation by LC /
MS using single ion monitoring with in - source fragmentation . -
pubmed
Decalcified sections were examined by
hematoxylin and
eosin ( HE ) staining ,
alkaline phosphatase (
ALPase ) immunohistochemistry and
tartrate - resistant acid phosphatase (
TRAPase ) enzyme histochemistry . -
pubmed
MS /
MS detection was performed by monitoring the parent-->daughter pair of
levosulpiride and the IS at m / z 342 - ->112 and 329 - ->256 , respectively . -
pubmed
[ A study of the catalytic properties of the
nitrile hydratase immobilized on
aluminum oxides and
carbon - containing adsorbents ] . -
pubmed
The activity and operational stability of the immobilized
nitrile hydratase were studied in the reaction of
acrylonitrile transformation into
acrylamide . -
pubmed
It was demonstrated that an increase in the
carbon content in the carrier led to an increase in the amount of adsorbed
enzyme and , concurrently , to a decrease in its activity . -
pubmed
The
nitrile hydratase immobilized on
Sibunit and
carbon - containing
aluminum alpha - oxide having a " crust " structure displayed the highest operational stability in
acrylonitrile hydration . -
pubmed
As a result , ten compounds were purified and three of them were identified as
3 - methyl-5 - vinyloxazolidin-2 - one ( compound A ) ,
5 - hydroxymethyl-2 - furaldehyde ( compound B ) and
3 - methylfuran-2 - carboxylic acid ( compound G ) based on the
MS , IR and extensive NMR data , respectively . -
pubmed
The aggregation of solutes occurs due to a specific
H (+ ) pump denominated
V - ATPase that maintains an inner acidic media ( pH 5.5 ) . -
pubmed
When this gradient is reduced by modulation of the
V - ATPase activity ,
catecholamines and
Ca ( 2 + ) are moved toward the cytosol . -
pubmed
Jack bean alpha - mannosidase digestion profile of hybrid - type
N - glycans : effect of reaction pH on substrate preference . -
pubmed
In this study , the
JBM digestion profile of hybrid - type
N - glycans was examined using
pyridylamino ( PA - ) sugar chains . -
pubmed
The digestion efficiencies of the
PA - labeled hybrid - type
N - glycans Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6 ( GlcNAcbeta1,2Manalpha1,3 ) Manbeta1,4GlcNAcbeta1,4GlcNAc - PA (
GNM5 - PA ) and
Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6 ( Galbeta1,4GlcNAcbeta1,2Manalpha1,3 ) Manbeta1,4GlcNAcbeta1,4GlcNAc - PA ( GalGNM5 -
PA ) were significantly lower than that of the
oligomannose - type
N - glycan Manalpha1,6 ( Manalpha1,3 ) Manalpha1,6Manbeta1,4GlcNAcbeta1,4GlcNAc - PA ( M4 -
PA ) , and the trimming pathways of
GNM5 - PA and
GalGNM5 - PA were different from that of
M4 - PA , suggesting a steric hindrance to the
JBM activity caused by
GlcNAcbeta1 - 2Man ( alpha ) residues of the hybrid - type
N - glycans . -
pubmed
The
ERalpha selective agonist ,
4,4 ' , 4 ''-[ 4 - propyl -( 1H )- pyrazole-1,3,5 - triyl ] tris - phenol (
PPT ; s.c . -
pubmed
The current evaluation of
glucuronide metabolites of
BUP and
norBUP are suggestive of combined inhibition of
Uridine diphosphate ( UDP )- glucuronosyltransferase of the
1A family and
cytochrome P450 3A4 that spares
UGT2B7 leading to a shunting of
BUP away from production of
norBUP and toward
BUP-3 G as seen by a statistically significant increase in the AUC of
BUP-3G. -
pubmed
Using atomic force microscopy on living cells , we show that
septin depletion significantly reduced the unbinding force of
InlB -
Met interaction and the viscosity of membrane tethers at locations where the
InlB -
Met interaction occurs . -
pubmed
Most available studies have addressed
steroid and
calcineurin inhibitor minimization in an attempt to prevent the development of
chronic allograft dysfunction and cardiovascular mortality . -
pubmed
It is known that
TSH binding to the
TSHR depends on the interaction with the
leucine - rich repeat and sulfation at Y385 of the hinge region . -
pubmed
Indeed , we identified further
amino acids of the hinge region with influence on
bTSH binding . -
pubmed
Moreover , side chain variations revealed that different
amino acid properties like the negative charge , aromatic as well as hydrophilic characteristics , contribute to maintain the hormone -
TSHR hinge interaction . -
pubmed
BACKGROUND :
Multiple sclerosis (
MS ) is associated with pathogenic autoimmunity primarily focused on major CNS - myelin target
antigens including
myelin basic protein (
MBP ) ,
proteolipidprotein (
PLP ) ,
myelin oligodendrocyte protein (
MOG ) . -
pubmed
This , together with previously demonstrated disease - susceptibility to
MBP and
MOG in DRB1 * 1501 - transgenics , also suggests a differential role for
DRB1 * 1501 and
DQB1 * 0602 depending on target
antigen and imply a potential complex ' genotype / target
antigen / phenotype ' relationship in
MS heterogeneity . -
pubmed
Stable - isotope dilution
GC -
MS method for
ethanol in vapour
ethanol and microdialysis systems based on
carbonate - catalyzed extractive pentafluorobenzoylation . -
pubmed
We present a novel
GC -
MS method for the accurate and precise analysis of
ethanol in cell cultures and microdialysates . -
pubmed
The method is based on the
carbonate - catalyzed extractive pentafluorobenzoylation of
ethanol and
deuterium - labelled
ethanol serving as the internal standard and on their
GC -
MS analysis in the electron - capture negative - ion chemical ionization mode . -
pubmed
All patients had the same , novel homozygous c.1580G>T
LMNA mutation , resulting in the replacement of
arginine 527 by
leucine . -
pubmed
Akt phosphorylates
HK - II at
Thr-473 and increases mitochondrial
HK - II association to protect cardiomyocytes . -
pubmed
Expression of an
HK - II - dissociating peptide diminished
IGF-1 - induced increases in mitochondrial
HK - II as well as protection against
hydrogen peroxide treatment , suggesting an important role of mitochondrial
HK - II in
IGF-1 /
Akt - mediated protection . -
pubmed
We hypothesized , on the basis of an
Akt phosphorylation consensus sequence present in
HK - II , that
Thr-473 is the target of
Akt kinase activity . -
pubmed
Indeed , recombinant
kinase - active
Akt robustly phosphorylates WT
HK - II , but not
Thr-473 mutants . -
pubmed
Phosphomimetic ( T473D)
HK - II , but not non - phosphorylatable ( T473A)
HK - II , constitutively increased mitochondrial binding compared with WT
HK - II and concomitantly confers greater protection against
hydrogen peroxide . -
pubmed
Glucose 6 - phosphate (
G-6P ) , a product of the catalytic activity of
HK - II , is well known to dissociate
HK - II from mitochondria . -
pubmed
Addition of
G-6P to isolated mitochondria dose - dependently dissociates WT
HK - II , and this response is inhibited significantly in mitochondria isolated from cardiomyocytes expressing T473D
HK - II . -
pubmed
Pretreatment with
IGF-1 also inhibits
G-6P - induced overexpressed or endogenous
HK - II dissociation , and this response was blocked by
Akt inhibition . -
pubmed
These results show that
Akt phosphorylation of
HK - II at
Thr-473 is responsible for the
Akt - mediated increase in
HK - II binding to mitochondria . -
pubmed
Tyrosinase inhibition activity was investigated using the
dopachrome method with
L-3,4 - dihydroxyphenylalanine (
L - DOPA ) as a substrate . -
pubmed
The
elastase inhibition activities of
ethanol ( 250 mug / ml ) and
ethyl acetate ( 125 mug / ml ) extracts were found to be significantly higher than that of the negative control . -
pubmed
The
tyrosinase inhibition activities of
ethanol and
ethyl acetate extracts , in terms of the mean inhibition concentration ( IC50 ) , were 1,078 and 1,467 mug / ml , respectively . -
pubmed
Substitution of
MOMP Thr(268 ) led to significantly reduced binding to
BgAgs . -
pubmed
The O - glycan moiety was characterized as
Gal ( beta1 - 3 )- GalNAc ( beta1 - 4 )- GalNAc ( beta1 - 4 )- GalNAcalpha1 - Thr(268 ) ; modelling suggested that O - glycosylation has a notable effect on the conformation of
MOMP and this modulates BgAg - binding capacity . -
pubmed
Increases in reactive
oxygen species enhance vascular endothelial cell migration through a mechanism dependent on the
transient receptor potential melastatin 4 ion channel . -
pubmed
The
transient receptor potential melastatin 4 (
TRPM4 ) protein is a ROS - modulated non - selective cationic channel that performs several cell functions , including regulating intracellular
Ca ( 2 + ) overload and
Ca ( 2 + ) oscillation . -
pubmed
Notably , we demonstrate that the
TRPM4 ion channel is critical in promoting
H2O2 - enhanced EC migration . -
pubmed
A
post - hoc analysis using automated analysis software revealed a significant reduction in crying symptoms in the PPI +
LLP group ( 99 [ 65 - 103 ] to 62 [ 32 - 96 ] episodes , P = 0.018 ) . -
pubmed
Fracture resistance of endodontically treated premolars restored with
lithium disilicate CAD / CAM crowns or onlays and luted with two luting agents . -
pubmed
A new ring expansion of
2 - aminobenzothiazoles with
alkynyl carboxylic acids was developed , which allows for one -
pot synthesis of
1,4 - benzothiazines in moderate to excellent yields . -
pubmed
In conclusion ,
warfarin treatment where patients spend a high proportion of time in the therapeutic range is safe and effective , and will continue to be a valid treatment option in the
era of newer oral anticoagulants . -
pubmed
TC ,
HDL - C ,
LDL - C and
TAG were measured , and
2,2 '- azinobis -( 3 - ethylbenzothiazoline-6 - sulfonic acid ) radical cation (
ABTS ( + ) ) scavenging capacity and
ferric reducing antioxidant power ( FRAP ) were determined in plasma . -
pubmed
NADPH oxidase (
NOX ) was also measured by
lucigenin - derived chemiluminiscence . -
pubmed
Onion ingredient improved antioxidant status in HCO group , as it was evidenced by
ABTS ( + ) and FRAP values and
SOD and
GPx enzyme activities compared to the HC - fed group , reduced the increment in
NOX activity and reversed
endothelial dysfunction promoted by the HC diet . -
pubmed
MAIN OUTCOME MEASURES : Longitudinal measurements of
AMH ,
FSH ,
LH ,
estradiol , and
inhibin B according to age , karyotype ( 45,X ; 45,X/46,XX mosaicism ; miscellaneous karyotypes ) , and ovarian status ( group 0 , prepubertal ; group 1 , never ovarian function ; group 2 , ongoing ovarian function ; and group 3 ,
loss of ovarian function ) . -
pubmed
After 14 days , the levels of
nitric oxide (
NO ) ,
nitric oxide synthase (
NOS ) ,
tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1beta (
IL-1beta ) ,
interleukin-10 (
IL-10 ) and
cyclic guanosine monophosphate (
cGMP ) in the spinal dorsal horn were measured by the corresponding kits , mRNA expression of
inducible NOS (
iNOS ) and
protein kinase G type I (
PKGI ) of spinal cord were analyzed by reverse - transcription
polymerase chain reaction ( RT - PCR ) . -
pubmed
Treatment with IGLR ( 200 , 400 , 800mg / kg ) significantly alleviated SNI - induced mechanical
allodynia , markedly decreased the levels of
NO ,
NOS ,
TNF - alpha ,
IL-1beta and
cGMP , and increased the level of
IL-10 . -
pubmed
Meanwhile ,
IGLR ( 200 , 400 , 800mg / kg ) also inhibited the protein expression of
NMDAR ,
PKCgamma and the mRNA expression of
iNOS and
PKGIota in the spinal cord . -
pubmed
CONCLUSIONS : These results indicated
IGLR could produce an anti - neuropathic
pain effect that might partly be related to the inhibition of the
NO /
cGMP /
PKG and
NMDAR /
PKC pathways and the level of
TNF - alpha ,
IL-1beta as well as to the increase of the level of
IL-10 in spinal cord . -
pubmed
At 1 year ,
high - density lipoprotein cholesterol (
HDL ) and
triglycerides (
TG ) improved similarly with all surgeries . -
pubmed
In contrast , the accessory elements on epsilon - II plasmids were typically located between
trfA and oriV , and contained IS1071 , which was commonly inserted within the Tn501 - like transposon , typically harboring a cluster of genes encoding
mercury resistance and/or catabolic pathways . -
pubmed
These activities have been attributed to its modulation of various molecular targets such as
cyclins , apoptosis- related proteins , proteasome activity , reactive
oxygen species , as well as
NF - kB ,
AKT /
mTOR and
MAPK /
ERK pathways . -
pubmed
Ibrutinib is a new - targeted therapy that irreversibly and specifically inhibits the
Bruton ' s Tyrosine Kinase (
BTK ) , a key component of the signaling pathways of B cells . -
pubmed
The loss of DNA associated with unwinding joint molecules previously observed in the absence of
RuvAB and
RecG , is suppressed by a
helicase deficient
PriA mutation (
priA300 ) , arguing that the action of
RecG ensures that
PriA is bound correctly on D - loops to direct DNA replication rather than to unwind joint
molecules . -
pubmed
CONCLUSIONS :
TAE plus
vincristine can rapidly improve levels of platelets and
fibrinogen , and it is an effective method for treatment of
corticosteroid - resistant
vascular tumors associated with
Kasabach - Merritt phenomenon in infants . -
pubmed
The
PL of
MoSe2 occurs in the near - infrared spectral range where the emissive properties do not suffer from the enhanced dissipation in the gold due to inter - band transitions . -
pubmed
Here , we study the interaction between monolayer
MoSe2 and plasmonic dipolar antennas in resonance with the
PL emission of
MoSe2 . -
pubmed
By varying the thickness of the spacer between the
MoSe2 layer and nanoantenna , we demonstrate manipulation of the
PL intensity from nearly fourfold quenching to approximately threefold enhancement . -
pubmed
The addition of MPG - medicated serum to the media of cultured GCs also reduced
cadmium chloride - induced apoptosis and downregulated
caspase-3 protein expression . -
pubmed
CONCLUSION : 20 micromol / L
XAV-939 and 20 mmol / L
LiCl as effective inhabitor and activator of
Wnt signalling pathway respectively can significantly down- and up - regulate the expression of Wnt down - stream pathway target genes and proteins . -
pubmed
, erosion , diffusion , and water - mediated processes ) were studied in four release media : PBST pH 7.4 ( standard condition ) , PBST pH 6.5 , PBS+1.0 %
triethyl citrate (
TC ) , and HBST pH 7.4 . -
pubmed
Aspirin plus
sorafenib potentiates
cisplatin cytotoxicity in resistant
head and neck cancer cells through
xCT inhibition . -
pubmed
The nonsteroidal anti - inflammatory drug
aspirin and the
multikinase inhibitor
sorafenib have both shown experimental and clinical anticancer activities . -
pubmed
The combination of
aspirin and
sorafenib induced
xCT inhibition ,
GSH depletion , and ROS accumulation in
cancer cells . -
pubmed
Genetic and pharmacological inhibition of
xCT potentiated the
cytotoxic effects of
aspirin plus
sorafenib ; this effect was diminished by
xCT overexpression . -
pubmed
Uric acid levels significantly rose with
body mass index (
BMI ) , waist circumference ( WC ) ,
triglyceride (
TG ) levels ,
triglycerides to
high - density lipoprotein cholesterol ratio (
TG /
HDL - C ) ,
insulin levels , homeostatic model assessment index ( HOMA - IR ) , high - sensitivity
CRP (
hs - CRP ) levels , and
leptin levels . -
pubmed
Conversely , the levels of
HDL - C and
adiponectin were negatively correlated with
uric acid levels . -
pubmed
In multiple regression analysis , there were age ,
BMI ,
TG /
HDL - C ratio , and
adiponectin levels remained significantly associated with
uric acid levels . -
pubmed
Our findings suggest that the anti - inflammatory action of
DXM via inactivation of the
TLR-4 /
NF - kappaB pathway , in part , may explain
DXM - induced neuroprotection after
cerebral ischemia . -
pubmed
Here , we demonstrate that
insulin temporally induces
Tnfaip8 , which mediates the anti - autophagic action of
insulin through formation of a novel ternary complex including
Tnfaip8 ,
phosphatidylethanolamine (
PE ) and
Galphai3 . -
pubmed
Intestinal lymphatic
HDL miR-223 and
ApoA - I are reduced during
insulin resistance and restored with
niacin . -
pubmed
The intestine is involved in whole - body lipid and
cholesterol homeostasis and secretes lipoproteins containing
apolipoprotein ( Apo ) B48 and discrete
ApoA - I into the mesenteric lymph . -
pubmed
The lymphatic system has been proposed to have a significant role in the reverse
cholesterol transport pathway associated with HDL -
ApoA - I. -
pubmed
Niacin (
nicotinic acid ;
vitamin B3 ) is known to regulate lipid metabolism , but the role of
niacin in modulating intestinal lipid and lipoprotein (
ApoB48 and
ApoA - I ) metabolism is unknown . -
pubmed
Intestinal lymph
HDL -
ApoA - I and miR-223 expression were lower by at least 45 and 60 % , respectively , and lymph
HDL was associated with 85 % higher
triglyceride (
TG ) content in IR compared to non - IR control group . -
pubmed
Niacin was found to increase secretion of lymph HDL and miR-223 by at least 50 - 60 % and to deplete the TGs associated with
HDL compared with the nontreated IR group . -
pubmed
Niacin significantly increased
peroxisome proliferator - activating nuclear receptor alpha and
carnitine palmitoyltransferase I alpha mRNA and annulled Tnf - alpha mRNA expression in intestinal ( jejunal ) explants . -
pubmed
Altered intestinal lymphatic HDL -
ApoA - I and miR-223 metabolism in IR and modulation by
niacin may provide insight into the intestinal - mediated regulation of the reverse
cholesterol transport pathway.-Mangat , R. , Borthwick , F. , Haase , T. , Jacome , M. , Nelson , R. , Kontush , A. , Vine , D. F. , Proctor , S. D. Intestinal lymphatic HDL miR-223 and
ApoA - I are reduced during
insulin resistance and restored with
niacin . -
pubmed
However , the
CRP rs1130864 A allele carrier state was significantly more frequent : ( i ) in
BD patients with
thyroid disorders than in those without ( pc = 0.046 ) , especially among females ( pc = 0.01 ) and independently of
lithium treatment , ( ii ) in
BD patients with rapid cycling than in those without ( pc = 0.004 ) . -
pubmed
BCS is caused by
hypercoagulability , e.g , arising from
malignancy ,
oral contraceptives , and
deficiency of protein S or C. It is not rare that
BCS often shows
venous thrombosis , including in superior vena cava . -
pubmed
When the
SRCHA was initiated by the trigger DNA , a series of DNA assembly steps based on the toehold - mediated DNA strand displacement were activated , and the Y shaped DNA (
P1 -
P2 -
P3 ) was formed . -
pubmed
High
parathyroid hormone levels (
PTH ) were associated with early - phase
Tc99m - sestamibi positivity in both primary ( p = 0.01 ) and
secondary hyperparathyroidism ( p = 0.03 ) but not with last phase ( p = 0.11 , p = 0.37 , respectively ) . -
pubmed
CONCLUSION : Pre - operative
PTH levels and size of the gland were major determinants of
Tc99m - sestamibi positivity on early - phase
Tc99m - sestamibi scans , whereas size is an independent predictor of late - phase
Tc99m - sestamibi positivity . -
pubmed
Quantification of
U-47700 and its metabolites in plasma by LC -
MS /
MS . -
pubmed
Serum
MDA ,
SOD , and
GSH - Px levels were detected . -
pubmed
CPE gavage obviously reduced FBG level , increased
body weight , enhanced
glucose tolerance , elevated FINS level and ISI , and reduced IRI , all in a dose - dependent manner . -
pubmed
The
cholinesterase inhibitor ,
donepezil (
Aricept ) , reverses AIE effects on dendritic spines , possibly by interacting with inflammatory and/or epigenetic mediators after AIE exposure . -
pubmed
All of these AIE effects were also reversed by
donepezil , with the exception of
HMGB1 . -
pubmed
In the present study , the roles of
AITC in up - regulating the
MRP1 expression and its relationship with the activation of the
Notch1 signaling pathway were investigated by combining the in vivo and in vitro experiments . -
pubmed
In contrast to the
COPD model group , the expressions of
MRP1 ,
Hes1 and
Notch1 dramatically up - regulated following the treatment with Low / High doses of
AITC . -
pubmed
The expression of
MRP1 in the 16
HBE cells was down - regulated by the inhibition of
Notch in a
DAPT concentration - dependent manner . -
pubmed
Additionally , the
AITC - induced up - regulation of the
MRP1 expression was markedly impaired following the inhibition of
Notch1 . -
pubmed
Biocompatible
poly - epsilon - caprolactone (
PCL ) ,
PCL / silk fibroin (
SF ) and
PCL /
SF /
Au(
SiO2 ) loaded nanofibrous scaffolds were first fabricated by an electrospinning method . -
pubmed
Osteogenic differentiation of MSCs into osteogenic cells were determined by
alkaline phosphatase (
ALP ) activity , mineralization by
alizarin red S (
ARS ) staining and
osteocalcin expression by immunofluorescence staining . -
pubmed
The results revealed that the addition of
SF and
Au(
SiO2 ) to
PCL scaffolds enhanced the mechanical strength , interconnecting porous structure and surface roughness of the scaffolds . -
pubmed
alpha - Linolenic acid but not
linolenic acid protects against
hypertension : critical role of
SIRT3 and autophagic flux . -
pubmed
In addition , SHRs displayed reduced vascular
Sirtuin 3 (
SIRT3 ) expression , subsequent
superoxide dismutase 2 (
SOD2 ) hyperacetylation and mitochondrial ROS overproduction , all of which were ameliorated by
ALA but not
LA supplementation . -
pubmed
In primary cultured endothelial cells ,
ALA treatment directly inhibited
SIRT3 reduction ,
SOD2 hyperacetylation , mitochondrial ROS overproduction and alleviated autophagic flux impairment induced by AngII plus
TNFalpha treatment . -
pubmed
However , these beneficial effects of
ALA were completely blocked by silencing
SIRT3 . -
pubmed
Restoration of autophagic flux by
rapamycin also inhibited mitochondrial ROS overproduction in endothelial cells exposed to AngII plus
TNFalpha . -
pubmed
Our findings suggest that
ALA but not
LA supplementation improves
endothelial dysfunction and diminishes experimental
hypertension by rescuing
SIRT3 impairment to restore autophagic flux and mitochondrial redox balance in endothelial cells . -
pubmed
After RNA extraction with precipitation method , the
Syber green qReal - time Polymerase Chain Reaction ( PCR ) was performed in order to evaluate the possible alteration in the expression of
IL-6 ,
BCL-2 , miR-181b-5p , and miR-23a-3p . -
pubmed
PLOD2 increases resistance of
gastric cancer cells to
5 - fluorouracil by upregulating
BCRP and inhibiting apoptosis . -
pubmed
The present study aimed to determine whether
PLOD2 could confer
5 - FU resistance in
GC . -
pubmed
Our mechanistic studies revealed that
PLOD2 - overexpressing cells appear to be resistant to the therapeutic characteristics of
5 - FU in
GC cells by upregulating
BCRP and that
PLOD2 confers resistance to
5 - FU - induced apoptosis in
GC cells by affecting the expression of
Bax and
Bcl2 . -
pubmed
Conclusion :
PLOD2 contributed to increasing resistance of
gastric cancer cells to
5 - fluorouracil by upregulating
BCRP and inhibiting apoptosis . -
pubmed
As a
NAD+-dependent deacetylase ,
SIRT1 is widely involved in apoptosis and cellular
inflammation via multiple pathways such as
p53 ,
NF - kB and
STAT . -
pubmed
The AVPI - bearing peptide assemblies in the cytosol sequestrate
IAPs and sensitize the
cancer cells to
bortezomib (
BTZ ) , a proteasome inhibitor , but rescue the normal cells ( i.e. -
pubmed
Alkaline phosphatase (
ALP ) acts as a context - dependent signal for trafficking the peptide /
BTZ assemblies and selectively inducing the
death of the
cancer cells . -
pubmed
Adropin regulates hepatic
glucose production via
PP2A /
AMPK pathway in
insulin - resistant hepatocytes . -
pubmed
Mechanistically ,
adropin dissociated
CREB -
CRTC2 and
FoxO1 -
PGC1alpha complex and reduced their binding to the promoters of
G6Pase and
PEPCK to decrease
glucose production in IR . -
pubmed
This study provides evidence that
adropin activates the
AMPK pathway via inhibition of
PP2A and decreases the liver
glucose production in IR context . -
pubmed
Two clones with higher - level resistance had the mutation pattern of Asn87Lys and one lower - level
LVX - resistant clone had an
Asp91Asn mutation . -
pubmed
Increase in the number of SNVs of the
Hp0970 (
hefE ) and
Hp1329 (
hefI ) genes change the resistance to
LVX . -
pubmed
Twelve mutations verified by Sanger sequencing in Hp0970 (
hefE ) and Hp1329 (
hefI ) may decrease resistant levels to
LVX . -
pubmed
Acetylcholine muscarinic receptors (
mAChRs ) contribute to both the facilitation and inhibition of
levodopa - induced
dyskinesia operated by striatal cholinergic interneurons , although the receptor subtypes involved remain elusive . -
pubmed
Cholinergic afferents from the midbrain also innervate the substantia nigra reticulata , although the role of
nigral mAChRs in
levodopa - induced
dyskinesia is unknown . -
pubmed
Reverse microdialysis allowed to deliver the
mAChR antagonists
telenzepine (
M1 subtype preferring ) ,
PD-102807 and
tropicamide ( M4 subtype preferring ) , as well as the selective
M4 mAChR positive allosteric modulator
VU0152100 in striatum or substantia nigra , while
levodopa was administered systemically . -
pubmed
The
M2 subtype preferring antagonist
AFDX-116 , used to elevate striatal
acetylcholine levels , blocked the behavioral and neurochemical effects of
PD-102807 . -
pubmed
In accordance with Article 6 of Regulation ( EC ) No 396/2005 , the applicants Bayer CropScience AG and Bayer
SAS submitted two requests to the competent national authority in the Netherlands to set import tolerances and to modify existing EU maximum residue levels ( MRLs ) for the active substance
flupyradifurone and its metabolite
difluoroacetic acid (
DFA ) in various crops . -
pubmed
In general , the safety profile of
tofacitinib is consistent with that of biologic disease - modifying anti - rheumatic drugs , with an increased risk of
herpes zoster that seems to be a class effect of
Janus kinase inhibitors . -
pubmed
Rethinking the
Citric Acid Cycle : Connecting
Pyruvate Carboxylase and
Citrate Synthase to the Flow of Energy and Material . -
pubmed
Here , we add the heart
lactate dehydrogenase proton - linked monocarboxylate transporter 1 complex , connecting the original
Citric Acid Cycle to the flow of energy and material . -
pubmed
The heart
lactate dehydrogenase proton - linked monocarboxylate transporter 1 complex catalyses the first reaction of the
Citric Acid Cycle , the oxidation of
lactate to
pyruvate , and thus secures the provision of
pyruvic acid . -
pubmed
Cycad Genotoxin
Methylazoxymethanol Disrupts the Brain
Ubiquitin - Proteasome Pathway ,
Tau and
alpha - Synuclein , as Reported in
ALS -
PDC . -
pubmed
These results provide in vivo evidence in support of the hypothesis that
adenosine caused inhibition of sympathetic neurotransmission via an action on
purinergic receptors located on sympathetic nerve terminals . -
pubmed
These data suggest that fluorescence is generated only through the coupling of
fluorescamine to the
N - terminal primary amine of
ATII . -
pubmed
The rate loss of biological activity of the
ATII -
fluorescamine derivative was appreciably lower than that observed for
ATII . -
pubmed
The present study suggests that the ATII -
fluorescamine derivative can be substituted for radioactively - labelled
ATII for use in a variety of applications . -
pubmed
Half of the subjects received 400 gamma of
fenoterol (
beta2 - sympathicomimetic ) , the other ones 200 gamma of
SCH 1000 ( synthetic
atropine ) . -
pubmed
Cross - linking of
troponin with
dimethylimido esters . -
pubmed
The topology of
troponin , the
calcium binding regulatory protein in muscle , has been studied by cross - linking with different length
dimethylimido esters . -
pubmed
Cross - linked
troponin has lost the ability to make the
actomyosin ATPase calcium sensitive although it does bind to
actin - tropomyosin and
tropomyosin , and it binds
calcium normally . -
pubmed
Whereas there was little detectable
45Ca2 + uptake into control cells in either the absence or presence of extracellular
ATP , a marked time - dependent uptake of
45Ca2 + was observed in the presence of
ATP in cells fused with
SR Ca - ATPase . -
pubmed
The newly incorporated native kidney pumps , while of the same isoform as the endogenous red cell pump , behave differently from the endogenous red cell
sodium pump with respect to their very low " uncoupled "
Na+/O flux activity . -
pubmed
Retinoids selective for
retinoid X receptor response pathways . -
pubmed
However , in the presence of
9 - cis - retinoic acid , a ligand for both
RARs and
RXRs ,
RXRs can also form
homodimers . -
pubmed
A series of
retinoids is reported that selectively activates
RXR homodimers but does not affect
RAR -
RXR heterodimers and thus demonstrates that both
retinoid response pathways can be independently activated . -
pubmed
[ Effects of
neurotropin on
seizure activity in
picrotoxin kindling ] . -
pubmed
Under conditions of acute
picrotoxin -- induced
seizures it was observed that preliminary
cycloheximide ( protein - synthesis blocker ) administration abolished anticonvulsant properties of
neurotropin . -
pubmed
Body weight ,
hemoglobin , hematocrit , and viable cell yield of pups were significantly decreased with decreasing dietary
iron . -
pubmed
As previously shown , in the presence of
tetrodotoxin ( 1 microM ) ,
acetylcholine ( 50 microM ) induces a prolonged stimulation of [ 3H]
dopamine release in both compartments through an interaction with
muscarinic receptors . -
pubmed
Our present study indicates that both
dynorphin 1 - 13 ( 1 microM ) and the selective kappa agonist
trans-3,4 - dichloro - N - methyl - N [ 2 -( 1 - pyrrolidinyl ) cyclohexyl ] benzeneace tamine (
U50488 ) ( 1 microM ) inhibit the
tetrodotoxin - resistant
acetylcholine - evoked release of
[ 3H ] dopamine , these effects being slightly more pronounced in the matrix than in the striosomal enriched area . -
pubmed
These results suggest that
dynorphin exerts an inhibitory presynaptic control of
dopamine release through
kappa opioid receptors located on
dopamine nerve terminals in the striosome as well as in the matrix . -
pubmed
A study was performed to examine the role of
prostaglandins (
PGs ) in the mechanism of the
ethanol - induced suppression of FBM , in which the objective was to test the hypothesis that fetal administration of
PGE2 can suppress the incidence of FBM following reversal of
ethanol - induced suppression of FBM by
indomethacin , a
fatty acid cyclooxygenase inhibitor . -
pubmed
FBM were suppressed during the period of
ethanol infusion ( 9.6 + /- 1.7 % ) ; the
ethanol - induced suppression of FBM was reversed by fetal
indomethacin treatment ( 77.5 + /-
14.1 % ) ; shortly after the onset of fetal
PGE2 infusion , the incidence of FBM decreased to a 2 - h mean incidence of
14.1 + /- 4.2 % , which was similar in magnitude to that observed after maternal
ethanol infusion . -
pubmed
Serum
PRL was significantly elevated through day 14 , while
E2 was elevated through day 7 by
E2 - CDS . -
pubmed
But much more [ 125I]
fibrin deposition was present in
CS reactions at 24 h than at 2 or 6 h after
Ag challenge . -
pubmed
The system was initially stabilized at a
sinus holding pressure (
HP ) of 100 mmHg and an average SAP of 107.5 mmHg . -
pubmed
The distribution of
HLA - A and - B types in the patients with enhanced class I expression did not relate to those
antigens previously detected more frequently on erythrocytes , B7(Bga ) , B17(Bgb ) , A28(Bgc ) ,
B8 or
A10 , and the enhancement was not associated with any particular
HLA types . -
pubmed
In this report activated lymphocytes are demonstrated by using an avidin -
biotin - peroxidase complex (
ABC ) method combined with autoradiography that identifies DNA synthesis and , thus , activation . -
pubmed
These findings agree well with the assumption that
CD8 - positive cells identify the foreign
antigen in the context of
class I antigens , whereas
CD4 - positive cells are found to be associated with the recognition of
MHC locus
II coded
HLA antigens . -
pubmed
When
octylglucoside lysates of
lactoperoxidase iodinated cells were applied to 80 - kDa - Sepharose columns , a polypeptide complex of 152/125 kDa was eluted with the synthetic peptide
GRGDSPC , but not with GRGESP . -
pubmed
The role of the thyroid gland in
sterility is not well known ;
hyperthyroidism may have an effect upon
estrogen secretion by decreasing the level of the free forms of
estradiol , resulting in a lowering or disappearance of the
LH ovulatory peak . -
pubmed
Feed digestibility and
nitrogen economy were not markedly affected but radioisotopic measurements demonstrated an increase in
albumin disappearance and catabolic rates , and plasma faecal clearance during the course of the
infection . -
pubmed
Helix Pomatia (
HPA ) and
carcinoembryonic antigen (
CEA ) staining were studied in 113 primary
breast carcinomas , 63 metastatic lymph nodes and 10 resected local
recurrences , using the
avidin -
biotin - peroxidase complex (
ABC ) method . -
pubmed
Treatment was monitored hormonally by the determinations of
LH ,
FSH ,
17 beta - estradiol ,
prolactin ,
progesterone ,
testosterone ( T ) ( total and free ) ,
androstenedione ( delta 4A ) ,
dehydroepiandrosterone sulfate (
DHEA - S ) , and
sex hormone - binding globulin (
SHBG ) every 2 days . -
pubmed
These studies suggest that
lactose may have an important function in stabilization of newborn intestinal
disaccharidase enzymes . -
pubmed
Using
rhodamine -
phalloidin fluorescence microscopy , we observed that normal hepatocytes showed a submembranous
F - actin distribution with focal short microfilaments extending into filopodia along the periphery of the cell . -
pubmed
Hepatocytes exposed to
AFB1 for several hours exhibited retraction of their cytoplasm within a prominent circumferential peripheral band of
F - actin microfilament bundles . -
pubmed
Both the blebbing and the polar aggregation of
F - actin bundles occurred prior to cell
death as shown by
lactate dehydrogenase release and
trypan blue exclusion . -
pubmed
Calcium - dependent regulation of
phosphorylase activation in a fast - twitch oxidative - glycolytic skeletal muscle . -
pubmed
This system provided an opportunity to compare the regulation of contraction and activation of
phosphorylase by extracellular and intracellular sources of
Ca2 + . -
pubmed
Blocking extracellular
Ca2 + entry promoted an inactivation of
phosphorylase and diminished the elevation of resting tension , which in untreated muscles ensues with the onset of
fatigue . -
pubmed
This appears to occur because , although
Ca2 + induces
phosphorylase activation , a subsequent , but rapid non -
Ca2 + -mediated event promotes inactivation , even while
Ca2 + -mediated contraction is being sustained . -
pubmed
Differences could also be stated for distinct
HLA antigen combinations comprising these relevant
antigens . -
pubmed
Evidence that
N6 - PIA acted as a high - affinity agonist against the
external adenosine receptor was provided by experiments in which 100 microM
caffeine , a known antagonist , competitively blocked its effect . -
pubmed
The immunoreactivity of
prothrombin was approximately 40,000 times less than that of
Pr2 on a molar basis . -
pubmed
Biosynthesis of adrenal
corticosteroids : energy metabolism and the
hydroxylases . -
pubmed
[ Relation between
beta - lipoprotein content in serum and plasma
vitamin E content . -
pubmed
Carbonic anhydrase analyses of the blood of subjects exposed to a
helium -
oxygen environment at seven atmospheres pressure . -
pubmed
[ Reduction of
post - prostatectomy
bleeding by medication ( experience with the antifibrinolytic agent
AMCA ) ] . -
pubmed
Moreover , the gonadotrope inhibition induced by
LHRH agonist with prolonged action can be of therapeutic use in
idiopathic precocious puberty ,
prostatic cancer and inhibition of spermatogenesis ( an association with
androgens being necessary in this last case ) . -
pubmed
Ceftriaxone was administered
IV at a dose of 2 grams daily , either as a single injection of 2 g or as two injections of 1 g each . -
pubmed
A comparison of a powerful
luteinizing hormone releasing hormone analogue agonist and
estrogen in the treatment of advanced
prostatic cancer . -
pubmed
Characterization of a chromatographically purified d 1.063 - 1.21 or
HDL density fraction from fatty - fibrous plaques demonstrated particles of between 60 and 120 A diameter , a characteristic
apolipoprotein A - I band by
SDS - polyacrylamide gel electrophoresis , and a
precipitin peak closely migrating with that for plasma
HDL by two - dimensional immunoelectrophoresis . -
pubmed
The d greater than 1.21 density fraction from plaques isolated by affinity chromatography on a Sepharose - anti -
apolipoprotein A - I column contained small amounts of phospholipid but no measurable
cholesterol . -
pubmed
The d 1.063 - 1.21 density fraction from plaques showed a significant increase in percent free
cholesterol and phospholipid contents and decrease in
cholesteryl ester content relative to plasma
HDL . -
pubmed
This increase in free
cholesterol could represent evidence for an anti - atherogenic mechanism wherein infiltrated
HDL removes
cholesterol together with
phospholipid from the arterial wall . -
pubmed
The ability of
substance P ( SP ) to regulate peak
benzyl - penicilloyl (
BPO)-specific
IgE antibody - forming cell ( AFC ) responses in vivo and the ability of
SP and other neuropeptides to regulate BPO - specific memory
IgE AFC responses induced in vitro was determined . -
pubmed
Inclusion of either
SP or
vasoactive intestinal peptide (
VIP ) , but not
neurotensin ,
serotonin ,
somatostatin , or
gastrin , in cultures suppressed these responses in isotype - specific , dose - dependent fashion ( approximately 70 % ) . -
pubmed
Secondary
steroid resistance in
asthma may arise in response to the release of
cytokines that activate
AP-1 and other
transcription factors that bind directly to
GR . -
pubmed
A similar effect may also be seen with high concentrations of
beta 2 - agonists that activate another
GR binding transcription factor ,
CREB . -
pubmed
Sequencing of part of this fragment revealed a gene (
acmA ) that could encode a polypeptide of 437
amino acid residues . -
pubmed
In
AcmA , these three repeats are separated by nonhomologous intervening sequences highly enriched in
serine ,
threonine , and asparagine . -
pubmed
The mean proportion of
LHRH neurons containing immunoreactive
Fos was higher in the brains of young compared to middle - aged females in association with both the preovulatory ( p & lt ; 0.01 ) and the
steroid - induced
LH surge ( p & lt ; 0.001 ) . -
pubmed
The time course of activation of
LHRH neurons was delayed in the brains of aging females , and the proportion of double - labeled
LHRH neurons remained elevated longer in the brains of young compared to middle - aged
steroid - treated females . -
pubmed
The proportion of
nitrogen in whey protein :
casein decreased from 85:15 to 54:46 during the 28 day time period . -
pubmed
We have demonstrated that during
anoxia intracellular
K + decreases significantly in nerve cells and that this
K + efflux is mediated by
K + channels that are sensitive to
ATP . -
pubmed
P2 purinoceptors modulating
noradrenaline release from sympathetic neurons in culture . -
pubmed
ATP ( 1 mM ) inhibited , whereas
2 - methylthio - ATP ( 30 microM ) , a
P2Y - selective purinoceptor agonist , increased electrically evoked release of [ 3H]
noradrenaline from chick sympathetic neurons . -
pubmed
The
P2X - selective purinoceptor agonist
alpha , beta - methylene - ATP ( 30 microM ) had no effect . -
pubmed
The
ATP - induced inhibition of release as well as the facilitation caused by
2 - methylthio - ATP was not affected by the selective
adenosine ( P1 ) receptor antagonist
8 -( p - sulfophenyl )- theophylline (
8 - PST ; 100 microM ) , but completely prevented by the non - selective
P2 antagonist
suramin ( 300 microM ) . -
pubmed
MR imaging of
PRL - secreting macro -
adenoma has the advantage of being able to demonstrate the relationship of the
tumor to the optic nerve and the cavernous
sinus . -
pubmed
Reactivation of
NADH dehydrogenase (
complex I ) inhibited by
1 - methyl-4 -( 4 '- alkylphenyl ) pyridinium analogues : a clue to the nature of the inhibition site . -
pubmed
Expression of the
neurotoxicity of
1 - methyl-4 - phenyl-1,2,3,6 - tetrahydropyridine , following oxidation to
1 - methyl-4 - phenylpyridinium ion (
MPP + ) , is believed to involve inhibition of mitochondrial electron transport from
NADH dehydrogenase (
complex I ) to
ubiquinone . -
pubmed
The low content of
ASG in atrial cells of the bats is probably an indication of low demand for the natriuretic hormone of the granule , because , in such animals , and particularly in flight , conservation of fluid and
electrolytes is of paramount importance . -
pubmed
As in the growth cone , such changes involve
Ca ( 2 +)-dependent pre- and postsynaptic processes , among which is the activation of
protein kinase C. -
pubmed
The membrane ectoenzyme
5 '- nucleotidase converts
5 '- AMP into
adenosine which , in the nervous tissue , plays an important role as intercellular messenger . -
pubmed
In the present report we have analyzed the distribution of
5 '- nucleotidase activity with
electron microscopy , using a
cerium - based method , at different postnatal histogenetic stages ( postnatal days (
PND ) 11 , 17 , 28 ) . -
pubmed
Cytochemical patterns prove that the
cerium - based method is suitable for demonstration of
5 '- nucleotidase - specific activity . -
pubmed
We conducted a double - blind placebo - controlled cross - over trial to study the effect of the opiate antagonist ,
naloxone , on psychometric function together with plasma
adrenocorticotrophic hormone (
ACTH ) ,
cortisol and
prolactin . -
pubmed
Cortisol and
ACTH showed a sharp and significant rise after
naloxone treatment in both
RA and normal subjects ( P & lt ; 0.001 and P & lt ; 0.01 ) , but no difference was observed between the two groups . -
pubmed
Although both
IFN - gamma and
penicillin induced aberrant chlamydial morphology and growth , we did not find evidence that these treatments elicited a classical stress response . -
pubmed
In addition , T - cell - mediated lysis of Chlamydia - infected target cells treated with
IFN - gamma or
penicillin or grown in
tryptophan - deficient media was examined . -
pubmed
The increase of
IFN gamma , the most active principle inducing
neopterin release , was below the concentration needed for
neopterin stimulation . -
pubmed
As part of our efforts to understand the regulation of intracellular
cAMP and to generate new targets for pharmacological intervention , we have cloned and characterized the first isozyme in a new family of
cyclic nucleotide phosphodiesterases ,
PDE8A. -
pubmed
PDE8A is most similar to
PDE4 ( 38.5 %
amino acid identity in the catalytic domain ) , but is clearly not a member of any of the seven known
PDE families . -
pubmed
The C - terminal 545
amino acids of
PDE8A ( the region shared among all splice variants ) were expressed in baculovirus . -
pubmed
The
cAMP hydrolytic activity of
PDE8A is not modulated by
cGMP at concentrations up to 100 microM. -
pubmed
PDE8A is insensitive ( up to 100 microM ) to a variety of
PDE inhibitors including
rolipram ,
zaprinast ,
vinpocetine ,
SKF-94120 , and
IBMX , but is inhibited ( IC50 = 9 microM ) by the
PDE inhibitor
dipyridimole . -
pubmed
To give
PDE8A a descriptive name that distinguishes it from the other two known high affinity
cAMP - specific phosphodiesterases (
PDE4 and
PDE7 ) , we denote
PDE8A as the high affinity
cAMP - specific and
IBMX - insensitive
PDE . -
pubmed
Regulation of endothelial cell
myosin light chain phosphorylation and permeability by
vanadate . -
pubmed
Vanadate significantly increased EC
MLC phosphorylation in a time - dependent manner ( maximal increase observed at 10 min ) and augmented both the
MLC phosphorylation and permeability responses produced by
thrombin , an agonist which rapidly increases
tyrosine kinase activities . -
pubmed
The
vanadate - mediated increase in
MLC phosphorylation was not associated with alterations in either
phosphorylase A Ser / Thr phosphatase activities or in cytosolic [
Ca2 + ] but was strongly associated with significant increases in EC
MLCK phosphotyrosine content . -
pubmed
These data suggest that
tyrosine phosphatase activities may participate in EC contractile and barrier responses via the regulation of the
tyrosine phosphorylation status of
EC MLCK . -
pubmed
The resolution limit for these crystals is 3.9 A. Crystals of
homoserine dehydrogenase grown in the presence of the co - factor
NAD + have the tetragonal space group P41212 or its enantiomorph P43212 . -
pubmed
Protein crystals grown in the presence of the product
L - homoserine and the inert
NAD+ analogue
3 - aminopyridine adenine dinucleotide belong to the monoclinic space group
P21 with unit - cell parameters a = 58.8 , b = 104.2 , c = 120.7 A , beta = 91.9 degrees . -
pubmed
The goal of this study was to test the hypothesis that the synthesis / release of
hydroxyl radical accounts for impaired
nitric oxide synthase - dependent dilatation of the basilar artery during
diabetes mellitus . -
pubmed
These findings suggest that impaired
nitric oxide synthase - dependent dilatation of the basilar artery during
diabetes mellitus may be related to the synthesis / release of
hydroxyl radical . -
pubmed
These results show that the antithrombotic activity of
naroparcil is dependent on modification in the plasma GAG profile which inactivates
thrombin via the
HCII . -
pubmed
Functional complementation cloning allowed us to identify the corresponding gene (
SAM3 ) , which encodes an integral membrane protein comprising 12 putative membrane spanning regions and belonging to the
amino acid permease family . -
pubmed
Among
amino acid permease members , the closest relative of
Sam3p is encoded by the
YLL061w open reading frame . -
pubmed
Disruption of
YLL061w was shown to specifically lead to cells unable to use
SMM as a
sulfur source . -
pubmed
Expression of
hCB1 cannabinoid receptors abolished the
Ca ( 2 + ) current inhibition by endogenous
pertussis toxin - sensitive
G ( i / o )- coupled receptors for
norepinephrine ( NE ) and
somatostatin (
SOM ) but not by endogenous
pertussis toxin - insensitive
G ( s )- coupled receptors for
vasoactive intestinal polypeptide . -
pubmed
Some
hCB1 receptors were constitutively active because the
cannabinoid receptor inverse agonist
SR 141617A enhanced the
Ca ( 2 + ) current . -
pubmed
Some
hCB1 receptors also appear to be precoupled to
G ( i / o )- proteins because the cannabinoid agonist
WIN 55,212 - 2 decreased the
Ca ( 2 + ) current at a time when no
G - proteins were available to couple to
alpha ( 2 )- adrenergic and somatostatin receptors . -
pubmed
In SCG neurons microinjected with a lower concentration of
hCB1 cDNA , the effect of
SR 141716A was reduced , and the response to NE and SOM was partially restored . -
pubmed
Troglitazone , a new oral antidiabetic drug , is reported to be mostly metabolized to its conjugates and not to be oxidized by
cytochrome P-450 (
P-450 ) enzymes . -
pubmed
Hydrophilic agents such as
carbon and
aluminum oxide stimulate ingrowth of fibroblasts that secrete
collagen . -
pubmed
To prevent , at least partly , priming for
dyskinesias can be attempted by delaying the need for
levodopa , and/or reducing the cumulative dose of
levodopa , using , for example ,
dopamine D2 agonists at the early stages of treatment . -
pubmed
This basic motif (
Lys ( 40 -) Lys - Leu - Lys - Lys(44 ) ) , conserved among all the pathway - specific
Smad proteins , is required for
Smad 3 nuclear import in response to ligand . -
pubmed
The effect of pH on the kinetics of the bioelectrocatalytic reduction of
H ( 2 ) O ( 2 ) catalysed by
horseradish peroxidase (
HRP ) has been studied at -50 mV vs. Agmid R :
AgCl on HRP - modified
Au electrodes placed in a wall - jet flow - through electrochemical cell . -
pubmed
A favourable adsorption of C(
His)rHRP on pre - oxidized
Au from a
protein solution at pH 6.0 provided a high and stable current response to
H ( 2 ) O ( 2 ) due to its bioelectrocatalytic reduction based on direct ( mediator - less ) electron transfer (
ET ) between
Au and the active site of
HRP . -
pubmed
The heterogeneous ET rate constant , k(s ) , calculated from experimental data on direct ET , on mediated ET in the presence of
catechol as well as from microbalance data , increased more than 30 times when changing from
nHRP to C(
His)
rHRP . -
pubmed
For both forms of
HRP , the increasing efficiency of bioelectrocatalysis with increasing
[ H ( 3 ) O (+ ) ] was observed . -
pubmed
The values of the apparent k(s ) between C(
His)rHRP and
Au changed from a value of 12+/-2 s(-1 ) in
PBS at pH 8.0 to a value of 434+/-62 s(-1 ) at pH 6.0 ; a similar k(s)-pH dependence was also observed for nHRP , providing the possibility to consider the reaction mechanism involving the participation of a proton in the rate - determining step of the charge transfer . -
pubmed
The apparent mass resolution of
oligonucleotides in time - of - flight (
TOF ) mass spectrometers has been examined . -
pubmed
Fasting lipid profiles ,
glucose and
insulin were also measured . -
pubmed
In
GH - deficient subjects , there were inverse correlations between
LDL cholesterol and
FMD ( r = -0.40 , P & lt ; 0.05 ) and between FMD and the augmentation index ( r = - 0.58 , P & lt ; 0.01 ) . -
pubmed
LDL cholesterol also decreased ( 3.5 + /- 0.8 vs. 4.2 + /- 0.8 mmol / l , P & lt ; 0.01 ) . -
pubmed
Reaction suspensions were separated by centrifugation , and absorption measurements made for separated
bilirubin ,
Fe , and
protein supernatants . -
pubmed
Concentrations of
bilirubin ,
Ferrum and
protein in supernatants decreased with increased
NaOCl concentration . -
pubmed
NaOCl had an effect on the
protein component in blood . -
pubmed
CsA concentrations were measured using a validated LC /
MS -
MS assay ( quantitation limit = 0.1 ng / mL ) . -
pubmed
Reversed - phase liquid chromatography method to determine
COL-3 , a
matrix metalloproteinase inhibitor , in biological samples . -
pubmed
In acini exposed to replication - defective or UV - inactivated Ad ,
carbachol - stimulated release of bulk protein and
beta - hexosaminidase were significantly ( P< ; or = 0.05 ) inhibited to an extent proportional to the loss of
rab3D - enriched mature secretory vesicles associated with these treatments . -
pubmed
Activation of
deoxycytidine kinase in lymphocytes is
calcium dependent and involves a conformational change detectable by native immunostaining . -
pubmed
Deoxycytidine kinase (
dCK ) , the principal
deoxynucleoside salvage enzyme , plays a seminal role in the bioactivation of a wide array of cytotoxic
nucleoside analogues . -
pubmed
BAPTA - AM , a cell - permeable
calcium chelator selectively inhibited the activation of
dCK in a time- and concentration - dependent manner while extracellular
calcium depletion had no effect . -
pubmed
Denaturing Western blots of extracts from lymphocytes incubated with
2 - chlorodeoxyadenosine ,
aphidicolin and/or
BAPTA - AM clearly demonstrated that
dCK protein levels were unchanged during these treatments . -
pubmed
Extracts from
CdA - treated cells were much better recognized by the antibody raised against the C - terminal peptide of
dCK than the
BAPTA -
AM - treated samples . -
pubmed
These results indicate that the
calcium - dependent activation of
dCK is accompanied by a conformational change that renders the C - terminal epitope more accessible to the antibody . -
pubmed
The mean population values of CL and
V1 of
gentamicin dosed once daily are in agreement with those described by others . -
pubmed
Using a selective
inducible nitric oxide synthase (
iNOS ) inhibitor ,
N ( 6 )-( iminoethyl )- lysine (
L - NIL ) , we explored the role of
iNOS in the early pro - inflammatory signaling and
acute lung injury in experimental
abdominal aortic aneurysm rupture . -
pubmed
Pulmonary permeability to
( 125 ) I - labeled
albumin ,
myeloperoxidase (
MPO ) activity ,
cytokine levels , and semi - quantitative RT - PCR for mRNA were indicators of microvascular permeability , leuco - sequestration , and pro - inflammatory signaling , respectively . -
pubmed
Lung tissue
MPO activity was significantly increased in s + c compared to sham ( 2.80 + /- 0.32 versus 1.03 + /- 0.29 , P & lt ; 0.002 ) , and attenuated by
L - NIL treatment ( 1.50 + /- 0.20 , P & lt ; 0.007 ) . -
pubmed
Lung tissue
iNOS activity was significantly increased in s + c compared to sham animals ( P & lt ; 0.05 ) , and attenuated by
L - NIL treatment ( P & lt ; 0.05 ) . -
pubmed
Therefore , trans - splicing of the
SL1 leader sequence may serve at least two functions in nematodes , generation of an optimal 5'-untranslated region length and a specific sequence context (
SL1 ) for optimal translation of
trimethylguanosine capped transcripts . -
pubmed
In all of the culture systems , inclusion of
ascorbic acid stimulated
PRG4 secretion , and the source of
PRG4 was immunolocalized to superficial cells . -
pubmed
The marked up - regulation of
PRG4 synthesis by
ascorbic acid may have implications for cartilage homeostasis and prevention of
osteoarthritic disease . -
pubmed
The skeletal muscle
calcium channel alpha - subunit gene
CACNA1S is a major disease - causing gene for
HypoPP , however , only three specific
HypoPP - causing mutations , Arg528His , Arg1,239His and Arg1,239Gly , have been identified in
CACNA1S to date . -
pubmed
Normal prostate tissue is regulated by
androgens , which activate the
androgen receptor , a nuclear receptor transcription factor . -
pubmed
Most
prostate tumors retain
androgen dependence , therefore , current therapies for advanced
prostate cancer either reduce
androgen levels or prevent binding to the
androgen receptor . -
pubmed
Although these
relapsed tumors are
androgen independent , they are still dependent on the
androgen receptor for their growth and survival . -
pubmed
METHODS : Cell proliferation was detected by
sulforhodamine B (
SRB ) assay after treated with
HY-1 at indicated doses . -
pubmed
Cell cycle analysis was performed by flow cytometry , mitochondria membrane voltage change was assessed by
rhodamine 123 staining ,
annexin V -
PI apoptosis detecting kit and DNA
agarose gel electrophoresis were used to identify apoptosis - inducing effect of
HY-1 . -
pubmed
It was recently reported that inhaled
NO causes peripheral vasodilatation after treatment with a
NO synthase (
NOS ) inhibitor . -
pubmed
These findings suggested the possibility that inhibition of endogenous
NOS uncovered the systemic vasodilating effect of
NO or
NO adducts absorbed via the lungs during
NO inhalation . -
pubmed
The
alpha2A - adrenoceptor antagonist
2 -(( 4,5 - dihydro-1H - imidazol-2 - yl ) methyl )- 2,3 - dihydro-1 - methyl-1H - isoindole (
BRL 44408 ) ( 1 mg kg(-1 ) ) prolonged the
hypothermic response to
MDMA . -
pubmed
Blood pressure actions of the three
amphetamine derivatives may be at least partly due to
alpha1 - adrenoceptor agonism or antagonism . -
pubmed
The reversal of the
hypothermic actions are at least partly due to
alpha2A - adrenoceptor agonism since the
hypothermic response was more prolonged with
MDEA which exhibits low
alpha2A - adrenoceptor potency , and effects of
MDMA after
alpha2A - adrenoceptor antagonism were similar to those of
MDEA . -
pubmed
Glutaredoxins (
GRXs ) can be subdivided into two subfamilies :
dithiol GRXs with the CPY / FC active site motif , and
monothiol GRXs with the CGFS motif . -
pubmed
cerevisiae cells have two monothiol GRXs with the
Trx -
Grx structure (
ScGrx3 and
ScGrx4 ) that locate at the nucleus and probably regulate the activity of transcription factors such as
Aft1 , and one monothiol GRX with the
Grx structure (
ScGrx5 ) that localizes at the mitochondrial matrix , where it participates in the synthesis of
iron - sulphur clusters . -
pubmed
PTP1B deficiency in immortalized neonatal hepatocytes prolonged
insulin - induced
tyrosine phosphorylation of the
insulin receptor (
IR ) and
IR substrates ( IRS ) - 1 , - 2 compared with wild - type control cells . -
pubmed
The ligand was shown to be
17beta - estradiol and the binding affinities of
EBP were demonstrated to be similar to those of
rat estrogen receptors . -
pubmed
Y - position
cysteine substitution in
type I collagen (
alpha1 ( I ) R888C / p. R1066C ) is associated with
osteogenesis imperfecta /
Ehlers - Danlos syndrome phenotype . -
pubmed
The most common mutations in
type I collagen causing types
II -
IV osteogenesis imperfecta (
OI ) result in substitution for
glycine in a
Gly - Xaa - Yaa triplet by another
amino acid . -
pubmed
Polyacrylamide gel electrophoresis ( PAGE ) of
[ ( 3 ) H ]- proline labeled steady - state
collagen reveals slight overmodification of the alpha1(I ) monomer band , much less than expected for a substitution of a neighboring
glycine residue , and a
faint alpha1(I ) dimer . -
pubmed
This report on Y - position substitutions in
type I collagen extends the range of phenotypes caused by
nonglycine substitutions and shows that , similar to X- and Y - position substitutions in
types II and III collagen , the phenotypes resulting from
nonglycine substitutions in
type I collagen are distinct from those caused by
glycine substitutions . -
pubmed
Calprotectin is a novel 36 kDa
calcium - binding protein of the
S100 family and appears to be a nonspecific marker of
inflammation . -
pubmed
Estrogens and antiestrogens activate
hPXR . -
pubmed
A significant proportion ( 54 % ) of compounds with estrogenic activity or able to bind
ER were found to be
hPXR activators : in particular , antiestrogens ,
mycoestrogens and
phthalates . -
pubmed
PAD patients often show elevated
triglycerides and reduced
HDL - cholesterol , two
lipid abnormalities usually accompanied by decreased
LDL size in the " atherogenic lipoprotein phenotype " (
ALP ) . -
pubmed
Patients had higher prevalence of
hypertension ( p=.0132 ) , smoking ( p<.0020 ) and
diabetes ( p=.0024 ) , with lower
HDL -
cholesterol ( p<.0001 ) and increased
triglycerides ( p=.0057 ) ;
LDL size was smaller ( p<.0001 ) , with decreased larger subclasses (
LDL - I , p<.0001 ;
LDL - IIA , p=.0068 ) and increased smaller particles (
LDL - IIIA , p<.0001 ;
LDL - IIIB , p=.0013 ;
LDL - IVA , p=.0029 ;
LDL - IVB , p<.0001 ) . -
pubmed
The concomitant presence of high
triglycerides , low
HDL -
cholesterol and elevated small , dense
LDL in patients was 26 % ( versus 0 % controls , p=.0024 ) . -
pubmed
2 '- Deoxycytidylate deaminase [ or
deoxycytidine-5 '- monophosphate ( dCMP ) deaminase ,
dCD ] catalyzes the deamination of
dCMP to
deoxyuridine-5 '- monophosphate to provide the main
nucleotide substrate for
thymidylate synthase , which is important in DNA synthesis . -
pubmed
Their
SOD activities were similar and 2- to 3 - fold more potent than the standard compound
EUK-134 . -
pubmed
Their
catalase - like activity was lower than that of
EUK-134 in the initial conversion rate ; however , some analogs exhibited a better catalytic turnover number . -
pubmed
This increase in MAP was associated with a significant increase in plasma
angiotensin - converting enzyme activity and
angiotensin II levels . -
pubmed
Effect of ZO pretreatment on radiation - induced changes in
glutathione (
GSH ) ,
glutathione - S - transferase (
GST ) ,
superoxide dismutase (
SOD ) ,
catalase (
CAT ) and lipid peroxidation ( LPx ) levels was also analyzed . -
pubmed
A significant ( p & lt ; 0.001 ) reduction in micronucleated polychromatic , normochromatic erythrocytes , increased PCE / NCE ratio , increase in the
GSH ,
GST ,
SOD ,
CAT and decreased LPx levels were observed in ZO pretreated group when compared to the irradiated animals . -
pubmed
It was not or only partially inhibited by several modulators of the cell surface
NTPDases , but was strongly inhibited upon oxidative cross - linking by
copper phenanthroline . -
pubmed
Moreover the
TS samples showed more antioxidant activity in some aspects and also exhibit more
flavonoid content . -
pubmed
However , in the second experiment , nine male and twelve female participants subjected to a cold pressor test (
CPT ) before and after Red Bull consumption showed a significant increase in blood
sugar levels pre- and post Red Bull consumption . -
pubmed
In addition , nanoencapsulation permits the cells to respond to changes in extracellular
glucose and other
insulin secretagogues by releasing
insulin with a profile similar to that of nonencapsulated cells . -
pubmed
This review examines the new evidence supporting the role of
nontriglyceride fatty acid metabolites in causing
NASH and how adipose and muscle
insulin resistance contribute to
hepatic lipotoxicity . -
pubmed
Injection of an anti -
PI ( 4,5 ) P ( 2 ) antibody inhibited invadopodia formation along with
gelatin degradation activity . -
pubmed
Sequestering of
PI ( 4,5 ) P ( 2 ) by overexpression of the
phospholipase C ( PLC ) delta1 -
pleckstrin homology ( PH ) domain , a specific probe for
PI ( 4,5 ) P ( 2 ) , also blocked invadopodia formation , while a mutated
PLCdelta1 - PH domain that lacks PI(4,5)P(2)-binding activity had no effect . -
pubmed
Cellular
PI ( 4,5 ) P ( 2 ) production is mainly mediated by
type - I phosphatidylinositol 4 - phosphate 5 - kinase ( PIP5KI ) family proteins , which include
PIP5KIalpha , Ibeta , and Igamma . -
pubmed
We screened a library of 37
indoles for those that selectively change fluorescence in the presence of prefibrillar amyloid - beta (
Abeta ) . -
pubmed
Using this probe in combination with electron microscopy , we found that prefibrils are largely depleted during
Abeta aggregation in vitro but that they remain present after the apparent saturation of the
ThT signal . -
pubmed
These results suggest that a combination of
TROL and
ThT provides greater insight into the process of amyloid formation by
Abeta . -
pubmed
In addition , we found that
TROL also recognizes other amyloid - prone proteins , including
ataxin-3 ,
amylin , and
CsgA. -
pubmed
The
brain cannabinoid ( CB ( 1 ) ) receptor is unique in that it lacks the interloop
E2 disulfide linkage to the transmembrane (
TM ) helical bundle , a characteristic of many
GPCRs . -
pubmed
Recent mutation studies of the
CB ( 1 ) receptor , however , suggest the presence of an alternative intraloop
disulfide bond between two
E2 Cys residues . -
pubmed
Considering the oxidation state of these
Cys residues , we determine the molecular structures of the 17 - residue
E2 in the
dithiol form (
E2 ( dithiol ) ) and in the
disulfide form ( E2(
disulfide ) ) of the
CB ( 1 ) receptor in a fully hydrated
1 - palmitoyl-2 - oleoyl - sn - glycero-3 - phosphocholine bilayer , using a combination of simulated annealing and molecular dynamics simulation approaches . -
pubmed
We characterize the
CB ( 1 ) receptor models with these two
E2 forms ,
CB ( 1 )( E2 ( dithiol ) ) and
CB ( 1 )( E2 ( disulfide ) ) , by analyzing interaction energy , contact number , core crevice , and cross correlation . -
pubmed
Dopamine ( DA ) produced by TIDA neurons directly inhibits
prolactin secretion and , therefore , to stimulate
prolactin secretion ,
estrogens inhibit TIDA neurons to decrease DA production . -
pubmed
Chronic E(2 ) exposure significantly increased the expression of
glial fibrillary acidic protein and the concentrations of
interleukin-1beta (
IL-1beta ) and
nitrate in the
AN that contains perikarya of TIDA neurons and increased nitration of
TH in the median eminence ( ME ) that contains the terminals . -
pubmed
PYR-41 is a small molecule with previously described
UBE1 inhibitory activity . -
pubmed
Detailed evaluation of
PYR-41 activity demonstrated that
PYR-41 inhibited
UBE1 activity but also had equal or greater inhibitory activity against several deubiquitinases ( DUBs ) in intact cells and purified
USP5 in vitro . -
pubmed
Both
UBE1 and
DUB inhibition were mediated through
PYR-41 - induced covalent protein cross - linking which paralleled the inhibition of the target proteins enzymatic activity . -
pubmed
PYR-41 also mediated cross - linking of specific protein kinases (
Bcr -
Abl ,
Jak2 ) to inhibit their signaling activity . -
pubmed
Blood samples were collected up to 58 hours
post - first dose for plasma concentrations of
modafinil and its metabolites . -
pubmed
Cy5.5 - Gly - Pro - Leu - Gly - Val - Arg - Gly -( TDOPA ) 3 - flower - like gold -
Fe3O4 optical nanoparticles Extracellular matrix ( ECM ) adhesion molecules consist of a complex network of
fibronectins ,
collagens , chondroitins ,
laminins , glycoproteins ,
heparin sulfate ,
tenascins , and proteoglycans that surround connective tissue cells , and they are mainly secreted by fibroblasts , chondroblasts , and osteoblasts ( 1 ) . -
pubmed
PEG - AuNPs have been being studied as
cancer CT imaging and photothermal agents ( 7 ) . -
pubmed
The peptide
Gly - Pro - Leu - Gly - Val - Arg - Gly - Cys - NH2 was found to be a
MMP substrate and is cleaved between the
Leu and
Gly residues . -
pubmed
NIR fluorescence signal will increase when the
Leu - Gly bond is cleaved by
MMPs , releasing
Cy5.5 - containing fragments . -
pubmed
Cy5.5 - MMP - AuNPs are being developed for NIR fluorescence imaging of
MMPs expressed in
tumors ,
atherosclerosis ,
myocardial infarction , and other diseases . -
pubmed
( 14 ) replaced the AuNP with
Au - Fe3O4 , a composite
NP shaped like a flower , to induce a fluorescently quenched state to the overall nanostructure . -
pubmed
The
MMP peptide was covalently linked to an anchoring unit ,
Lys - tridihydrophenylalanine (
Lys - TDOPA ) on the surface of the
iron oxide NP . -
pubmed
Cy5.5 - Gly - Pro - Leu - Gly - Val - Arg - Gly -( Lys - TDOPA)3 - flower - like gold -
Fe3O4 optical NPs ( FANPs ) have been evaluated for imaging
protease expression in vivo . -
pubmed
VEGF - D promotes
tumor metastasis by regulating
prostaglandins produced by the collecting lymphatic endothelium . -
pubmed
We identified regulation of
PGDH , the key enzyme in
prostaglandin catabolism , in endothelial cells of collecting lymphatics , as a key molecular change during
VEGF - D - driven
tumor spread . -
pubmed
The
VEGF - D - dependent regulation of the
prostaglandin pathway was supported by the finding that collecting lymphatic vessel dilation and subsequent
metastasis were affected by nonsteroidal anti - inflammatory drugs ( NSAIDs ) , known inhibitors of
prostaglandin synthesis . -
pubmed
Our data suggest a control point for
cancer metastasis within the collecting lymphatic endothelium , which links
VEGF - D /
VEGFR-2 /
VEGFR-3 and the
prostaglandin pathways . -
pubmed
Over the past 5 y , research has revealed that the basic
leucine zipper superfamily of
transcription factors plays the central role in the
AAR . -
pubmed
Measured FTIR ,
CP / MAS , and high - resolution
( 13 ) C NMR spectra indicate that H - bond in the
PyO HCl complex in polar solvent can potentially be more ionic than in the crystal . -
pubmed
RESULTS : At 7 d after the first
SAH , protein levels of
phospho -
Akt and
ERalpha were significantly decreased and
caspase-3 was significantly increased in the dentate gyrus . -
pubmed
Those actions were reversed by
E2 and blocked by
ICI 182,780 and
methyl - piperidino - pyrazole , but not
R , R - tetrahydrochrysene . -
pubmed
However , there were no significant changes in the expression of the protein levels of
phospho -
Akt ,
ERalpha ,
ERbeta , and
caspase-3 , and DNA fragmentation after
SAH . -
pubmed
CONCLUSIONS : The present study shows that a beneficial effect of
E2 in attenuating
SAH - induced apoptosis is associated with activation of the expression of
phospho -
Akt and
ERalpha , and alteration in
caspase-3 protein expression via an
ERalpha - dependent mechanism in the dentate gyrus . -
pubmed
Melatonin selectively activated
Gq and stimulated
phosphoinositide (
PI ) hydrolysis in freshly dispersed and cultured muscle cells . -
pubmed
Melatonin - induced
PI hydrolysis and contraction were blocked by a non - selective
MT1 /
MT2 antagonist
luzindole ( 1 muM ) , but not by a selective
MT2 antagonist
4P - PDOT ( 100 nM ) , and by the
PLC inhibitor
U73122 . -
pubmed
MT2 selective agonist
IIK7 ( 100 nM ) had no effect on
PI hydrolysis and contraction . -
pubmed
Activation of these receptors causes stimulation of
PI hydrolysis and increase in cytosolic
Ca ( 2 + ) , and elicits muscle contraction . -
pubmed
Product analyses were performed with the application of ESI -
MS and
( 1 ) H - NMR techniques . -
pubmed
In cellular systems , two inhibitors ,
KH7 and
catechol estrogens , proved specific for
sAC , and
2 ' , 5 '- dideoxyadenosine proved specific for
tmACs . -
pubmed
Insulin sensitivity and beta - cell function were evaluated by oral
glucose tolerance testing . -
pubmed
Here we report a
MOF that consists of
glycyl - serine dipeptides coordinated to metal centres , and has a structure that evolves from a solvated porous state to a desolvated non - porous state as a result of ordered cooperative , displacive and conformational changes of the peptide . -
pubmed
First - line triplet chemotherapy plus
bevacizumab ( FIr - B / FOx ) can improve efficacy of metastatic
colorectal cancer (
MCRC ) ,
KRAS wild - type and mutant . -
pubmed
The design and synthesis of novel
SGLT2 inhibitors :
C - glycosides with
benzyltriazolopyridinone and
phenylhydantoin as the
aglycone moieties . -
pubmed
This report describes the design , synthesis and structure - activity relationship (
SAR ) of
C - glycosides with
benzyltriazolopyridinone and
phenylhydantoin as the
aglycone moieties as novel
SGLT2 inhibitors . -
pubmed
Is preference for ovulatory female 's
faces associated with men 's
testosterone levels ? -
pubmed
Testosterone was not predictive for the chosen
face ; regardless of
testosterone level men preferred the ovulatory woman . -
pubmed
There have been many new developments for treatment of advanced
NETs including
somatostatin analogs , hepatic artery embolization , chemotherapy , interferons ,
mammalian target of rapamycin inhibitors and radiolabeled
somatostatin analogs . -
pubmed
While the ' C5100U5101 ' natural mutations in the upstream
ORF1 coding region replace '
alanine ' for the conserved '
valine ' , the ' G5117G5118 ' doublet resides in the downstream non - coding / promoter region of
ORF3 gene . -
pubmed
dianthus to 350 m depth (
pHT 8.02 ; pCO2 448 muatm , Omegaara 2.58 ) and to a 3 m depth
CO2 seep in oligotrophic waters (
pHT 7.35 ; pCO2 2879 muatm , Omegaara 0.76 ) and found that the transplants calcified at the same rates regardless of the pCO2 confirming their resilience to acidification , but at significantly lower rates than corals that were fed in aquaria . -
pubmed
Further , using cell cycle analysis ,
MTT , luciferase and knockdown experiments , we found evidence to suggest that
ETS1 is central in the development of the proliferative , metastatic and invasive phenotype of
ccRCC cells . -
pubmed
Purified polysaccharide SSP
II - a was successfully obtained using
diethylaminoethanol - Sepharose and Sepharose CL-6B column chromatography . -
pubmed
SSP
II - a was found to be an acidic polysaccharide fraction with an average molecular weight of 168kDa and composed of
rhamnose ,
glucuronic acid ,
galacturonic acid ,
glucose ,
galactose and
arabinose . -
pubmed
In vitro antioxidant activity assays suggested that SSP
II - a presents high scavenging activity toward
superoxide anion ,
hydroxyl , and
2,2 '- azino - bis ( 3 - ethylbenz - thiazoline-6 - sulfonic acid ) radicals but relatively lower scavenging activity toward
1,1 - diphenyl-2 - picrylhydrazyl radicals . -
pubmed
A Case of
Squamous Cell Carcinoma Harboring an
EML4 -
ALK Rearrangement that Was Unsuccessfully Treated with the
ALK Inhibitor
Alectinib . -
pubmed
BACKGROUND : To assess differences between four different voxel - based dosimetry methods ( VBDM ) for
tumor , liver , and lung absorbed doses following
( 90 ) Y microsphere selective internal radiation therapy ( SIRT ) based on
( 90 ) Y bremsstrahlung SPECT /
CT , a secondary objective was to estimate the sensitivity of liver and lung absorbed doses due to differences in organ segmentation near the liver - lung interface . -
pubmed
Special attention is given to the involvement of
pigment epithelial cells , macrophages , hyalocytes , gliocytes , supporting fibers ( Muller 's cells ) , mediators of
inflammation , such as
cytokines ,
chemokines , and
growth factors , with reference to their interaction in the processes of proliferation and development of retinal membranes . -
pubmed
This study compared the efficacy and safety of fixed dose combination (
FDC ) of
arterolane maleate and
piperaquine phosphate (
PQP ) with
chloroquine in the treatment of uncomplicated
vivax malaria . -
pubmed
The median parasite clearance time was 24 h in
FDC of
arterolane maleate and
PQP group and 26 h in
chloroquine group ( Log - rank , p = 0.2264 ) . -
pubmed
In
PP population , day 28 cure rates were 100 % in both the groups ( 95 % CI ( 96.52 , 100.0 for
FDC of
arterolane maleate and
PQP and 96.73 , 100.0 in
chloroquine group ) ) . -
pubmed
Incidence of adverse events was 82.4 % in the
FDC of
arterolane maleate and
PQP group and 85.4 % in the
chloroquine group . -
pubmed
The commonly reported clinical adverse events in the
FDC of
arterolane maleate and
PQP versus
chloroquine group were
vomiting ( 5.0 vs 5.1 % ) ,
headache ( 1.3 vs 3.2 % ) and prolonged QT ( 1.9 vs 3.2 % ) . -
pubmed
The pharmacokinetic analysis indicates that
arterolane maleate is well absorbed and has a relatively short t1/2 of 3.2 h.
Piperaquine is also well absorbed after oral administration with a t1/2 of about 228.33 h. CONCLUSIONS : The study showed that
FDC of
arterolane maleate and
PQP effectively cured
vivax malaria and attained acceptable level of cure up to day 28 . -
pubmed
The
Cytoplasmic Carbonic Anhydrases betaCA2 and
betaCA4 Are Required for Optimal Plant Growth at Low
CO2 . -
pubmed
We hypothesize that cytoplasmic
betaCAs are required to modulate inorganic
carbon forms needed in leaf cells for
carbon - requiring reactions such as photosynthesis and
amino acid biosynthesis . -
pubmed
Comparing transfer DNA knockout lines with wild - type plants , there was no reduction in the growth rates of the single mutants ,
betaca2 and
betaca4 However , the growth rate of the double mutant ,
betaca2betaca4 , was reduced significantly when grown at 200 muL L(-1 )
CO2 The reduction in growth of the double mutant was not linked to a reduction in photosynthetic rate . -
pubmed
This suggests the cytoplasmic
CAs play an important but not previously appreciated role in
amino acid biosynthesis . -
pubmed
BACKGROUND : Misuse and/or dependence upon non - prescription combination analgesics containing
codeine (
NP -
CACC ) can result in serious physiological and psychological harms . -
pubmed
LC -
MS /
MS analysis of
steroids in the clinical laboratory . -
pubmed
Liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) is a powerful tool that is changing the way we analyse
steroids in the clinical laboratory . -
pubmed
The
chromobox 7 (
CBX7 ) protein of the
polycomb repressive complex 1 (
PRC1 ) functions to repress transcription of
tumor suppressor
p16 ( INK4a ) through long noncoding RNA ,
ANRIL ( antisense noncoding RNA in the
INK4 locus ) directed chromodomain ( ChD ) binding to trimethylated
lysine 27 of
histone H3 ( H3K27me3 ) , resulting in chromatin compaction at the
INK4a /
ARF locus . -
pubmed
Our Class A compounds , a series including analogues of the previously reported MS452 , inhibit
CBX7ChD /
methyl - lysine binding by occupying the H3K27me3 peptide binding site , whereas our Class B compound , the newly discovered MS351 , appears to inhibit H3K27me3 binding when
CBX7ChD is bound to RNA . -
pubmed
Our crystal structure of the
CBX7ChD /
MS351 complex reveals the molecular details of ligand recognition by the aromatic cage residues that typically engage in
methyl - lysine binding . -
pubmed
Thus ,
MS351 represents a new class of ChD antagonists that selectively targets the biologically active form of
CBX7 of the
PRC1 in long noncoding RNA- and H3K27me3 - directed gene transcriptional repression . -
pubmed
PURPOSE : To investigate the effect of blocking
renin -
angiotensin system by direct
renin inhibition using
aliskiren on the
renal dysfunction following reversible
unilateral ureteral obstruction (
UO ) . -
pubmed
Group - Alsk ( n=12 ) received
aliskiren ( 30 mg / kg / day ) dissolved in water starting one day before creating UO and continued until the terminal experiment five days
post reversal when renal functions were measured using clearance techniques . -
pubmed
Aliskiren also decreased the gene expressions of
NGAL ,
KIM-1 and
p53 . -
pubmed
CONCLUSION : Direct
renin inhibition by
aliskiren appears to have protective effect on the
renal dysfunction and on the markers of
renal injury following UO indicating a potential clinical benefit of this agent . -
pubmed
The inhibitor
ifenprodil , and related
phenylethanoamine compounds specifically targeting
GluN1 /
GluN2B NMDARs have neuroprotective activity . -
pubmed
We applied genetically encoded unnatural
amino acids ( Uaas ) and monitored the functional changes in living cells with photo - cross - linkers specifically incorporated at the
ifenprodil binding interface between
GluN1 and
GluN2B subunits . -
pubmed
We report constraining the
NTD domain movement , by a light induced crosslinking bond that introduces minimal perturbation to the ligand binding , specifically impedes the transduction of
ifenprodil but not
Zn2 + inhibition . -
pubmed
A particularly versatile
CBT is
6 - amino-2 - cyanobenzothiazole (
ACBT ) , which has an
amine handle for straight - forward derivatisation . -
pubmed
EPR - juice had 9- and 5 - fold higher contents of
phenolic compounds compared with the NEB- and EB - juices , respectively . -
pubmed
Effect of the low content and stability of
phenolics was noticed as loss of the visual color in the
NEB - juice during storage . -
pubmed
The decrease in monomeric
anthocyanins and the increase in
phenolic acids were most severe in the
NEB - juice , whereas the most significant decline in
hydroxycinnamic acid derivatives occurred in the
enzyme - treated juices . -
pubmed
Formation of Hexagonal - Close Packed (
HCP )
Rhodium as a Size Effect . -
pubmed
Previous studies on the structural and functional properties of
rhodium are based on the
face - centered - cubic ( fcc ) structure in the bulk form . -
pubmed
The
NO donor prodrug
JS - K (
O2 -( 2.4 - dinitrophenyl ) 1 -[( 4 - ethoxycarbonyl ) piperazin-1 - yl ] diazen-1 - ium-1,2 - diolate ) allows cell - type specific intracellular
NO release via enzymatic activation by
glutathione - S - transferases overexpressed in
glioblastoma multiforme . -
pubmed
Increases in the intracellular
Ca2 + concentration in pancreatic islets , resulting from the
Ca2 + mobilization from the intracellular source through the
ryanodine receptor , are essential for
insulin secretion by
glucose . -
pubmed
Cyclic ADP - ribose , a potent
Ca2 + mobilizing second messenger synthesized from
NAD + by
CD38 , regulates the opening of
ryanodine receptor . -
pubmed
This report outlines the construction of an advanced , exquisite photoswitchable enzyme cascade on the basis that
tyrosinase (
TYR ) catalyzes the generation of
dihydroxyphenylalanine (
DOPA ) coordinated
TiO2 nanoparticles ( NPs ) to form a light responsive nano - trigger that subsequently photoactivates the enzymatic activity of
horseradish peroxidase (
HRP ) . -
pubmed
Prostate cancer stem - like cells ( PCSCs ) are not only enriched in the
CD44+
PSA-/lo subpopulation but also employ
androgen - independent signaling mechanisms for survival . -
pubmed
CD44 + PCSCs defy
androgen deprivation , resist chemo- and radiotherapy and are highly tumorigenic . -
pubmed
Among a subset of
tyrosine kinases crucial for
PCSC survival , was a non - receptor
tyrosine kinase ,
ACK1 /
TNK2 , a critical regulator of castration resistant
prostate cancer (
CRPC ) growth . -
pubmed
Consistently , activated
ACK1 as measured by phosphorylation at
Tyr284 was significant in the
CD44+
PSA-/lo population . -
pubmed
During adaptive evolution on the first
carbon source ,
4 - Hydroxyphenylacetic acid (
HPA ) , recombining populations adapted better , which was likely mediated by acquiring the
hpa operon from the donor . -
pubmed
Typical alpha - proteobacterial methylotrophy genes were found :
pyrroloquinoline quinone ( PQQ )- dependent methanol dehydrogenase (
MDH ) (
mxaF and
xoxF1 - 4 ) , methylotrophy regulatory proteins (
mxbDM and
mxcQE ) ,
PQQ synthesis ,
H4F pathway ,
H4MPT pathway ,
formate oxidation ,
serine cycle , and
ethylmalonyl - CoA pathway . -
pubmed
SDS - PAGE and subsequent LC -
MS analysis , and qPCR analysis revealed that
MxaF and
XoxF1 were the dominant
MDH in the absence or presence of
lanthanum (
La3 + ) , respectively . -
pubmed
The growth of
MDH gene - deletion mutants on
alcohols and qPCR results indicated that
mxaF and
xoxF1 are also involved in
ethanol and
propanol oxidation ,
xoxF2 participates in
methanol oxidation in the presence of
La3 + , while
xoxF3 was associated with
methanol and
ethanol oxidation in the absence of
La3 + , implying that
XoxF3 is a
calcium (
Ca2 +)-binding
XoxF. -
pubmed
Four
Ln3 + such as
La3 + ,
cerium (
Ce3 + ) ,
praseodymium (
Pr3 + ) , and
neodymium (
Nd3 + ) served as cofactors for
XoxF1 by supporting
DeltamxaF growth on
methanol . -
pubmed
Here , we show that proteolytic destruction of the key regulator
Mcl-1 during an extended mitosis requires the
anaphase - promoting complex or cyclosome (
APC / C ) and is independent of another ubiquitin
E3 ligase ,
SCFFbw7 Using live - cell imaging , we show that the loss of
Mcl-1 during mitosis is dependent on a D box motif found in other
APC / C substrates , while an
isoleucine -
arginine ( IR ) C - terminal tail regulates the manner in which
Mcl-1 engages with the
APC / C , converting
Mcl-1 from a
Cdc20 - dependent and checkpoint - controlled substrate to one that is degraded independently of checkpoint strength . -
pubmed
Gender Differences in the
Hepatotoxicity and Toxicokinetics of
Emodin : The Potential Mechanisms Mediated by
UGT2B7 and
MRP2 . -
pubmed
A genome - wide association study ( GWAS ) identified that rs11726899 located within ~50 kb of the transcript of
UGT2B could significantly affect
emodin metabolism . -
pubmed
Therefore , gender differences in the
hepatotoxicity and toxicokinetics of
emodin are potentially mediated by the coupling of
UGT2B7 and
MRP2 in vivo . -
pubmed
AIM : To study the influence of
adenosine receptor ligands and
hypoxic preconditioning on
carbohydrate metabolism in global
brain ischemia . -
pubmed
RESULTS AND CONCLUSION :
Adenosine , an agonist of
A1 adenosine receptors , and
hypoxic preconditioning exert a neuroprotective effect on brain cells . -
pubmed
The blocker of
A1 adenosine receptors aggravates metabolic disturbances in the brain and removes the protective effect of
cyclopenyladenosine and ischemic preconditioning . -
pubmed
Although , mortality of
MSC - TV group decreased 14.7 % as compared to
TAA group ( p = 0.200 ) ,
MSC + Scaffold group had the lowest mortality ( 31.4 % ) ( p = 0.013 ) . -
pubmed
The expression of
Notch2 and
Sox2 during extensive - passage , low -
oxygen culture and differentiation conditions were analyzed to confirm that the presence of these signature proteins directly correlates with the ability of NSCs to form new neurospheres and differentiate into multiple cell types . -
pubmed
The
glycosylphosphatidylinositol (
GPI ) anchored glycoprotein
Thy-1 has been prevalently expressed on the surface of various cell types . -
pubmed
The rapid recovery phase seen in the absence of
calcium , was dependent on extracellular
bicarbonate and could be inhibited by
S0859 , a potent
Na / HCO3 cotransporter inhibitor . -
pubmed
Immunostaining and PCR data , showed that
NBCe1 (
SLC4A4 ) and
NBCn1 (
SLC4A7 ) were expressed in the cell population and that the pHi recovery in the radial glial - like cells after
calcium removal was mediated mainly by the electrogenic
sodium bicarbonate transporter NBCe1 (
SLC4A4 ) . -
pubmed
Our results indicate that extracellular
calcium might hamper pHi regulation and
Na / HCO3 cotransporter activity in a
brain injury microenvironment . -
pubmed
In this study , we demonstrate that the partition of
proteins in salt - containing ATPS ( SATPS ) depends only on the relation between the
protein - surface
amino acids and the type of salt using SATPS . -
pubmed
Interestingly , the partition coefficients of the
proteins correlated to those of the combination of the
amino acids in the surface of the
protein with the correlation coefficients of & gt;0.9 . -
pubmed
It shows a splendid limit of detection of soluble
arsenic down to a few ppb level , much below than the
MCL reported value , without being interfered by any other ions . -
pubmed
FDG PET /
CT Findings of Unsuspected
Pulmonary Epithelioid Angiosarcoma Manifested as a Brain Metastasis . -
pubmed
FDG PET /
CT showed increased uptake corresponding to lesions in the right frontal lobe of the brain , the left upper lobe of lung , and the left adrenal gland , respectively . -
pubmed
To analyze the mechanisms implicated in autotolerance failure , we evaluated
CD4+
CD69 + and
CD4+
CD25+
FoxP3 + T cells and
interleukin profiles in a
pristane - induced
SLE experimental model . -
pubmed
Thus , the goal of this study was to assess the relationships among serum
estradiol (
E2 ) and
endothelial growth factor (
VEGF ) levels , MDSC ratios , and pregnancy outcome associated with
IVF . -
pubmed
Forage legumes - grazing lambs outperformed
SI lambs in LT nutritional quality , with more conjugated
linoleic acids and
n-3 PUFAs , especially
18:3n-3 ,
eicosapentaenoic and docosahexaenoic acids ( P & lt ; 0.001 ) , and thus lower
n-6 PUFA /
n-3 PUFA and 18:2 n-6/18:3 n-3 ratios ( P & lt ; 0.001 ) . -
pubmed
The liquid chromatography - mass spectrometry ( LC -
MS ) analysis of EAF demonstrated the presence of
phenolic metabolites that belong to three structurally related groups as majority compounds :
caffeoylquinic acid ,
coumaroyl - glucoside , and
caffeoyl - glucoside /
deoxyhexosyl - caffeoyl glucoside derivatives . -
pubmed
The following low - pressure limiting rate coefficients were obtained for T = 120 - 400 K and
He bath gas ( in cm6 molecule-2 s-1 , uncertainty + -sigma at 180 K ): log10(k ,
Ni+ +
N2 ) = -27.5009 + 1.0667log10(T ) - 0.74741(log10(T))2 , sigma = 29 % ; log10(k ,
Ni+ + O2 ) = -27.8098 + 1.3065log10(T ) - 0.81136(log10(T))2 , sigma = 32 % ; log10(k ,
Ni+ +
CO2 ) = -29.805 + 4.2282log10(T ) - 1.4303(log10(T))2 , sigma = 28 % ; log10(k ,
Ni+ +
H2O ) = -24.318 + 0.20448log10(T ) - 0.66676(log10(T))2 , sigma = 28 % ) . -
pubmed
Parthenolide Has Negative Effects on In Vitro Enhanced Osteogenic Phenotypes by Inflammatory Cytokine
TNF - alpha via Inhibiting
JNK Signaling . -
pubmed
PTL had no significant effects on hPDC viability or osteoblastic activities , whereas
TNF - alpha had positive effects on the in vitro osteoblastic differentiation of hPDCs . -
pubmed
Treatment with 1 microM
PTL did not affect
TNF - alpha - treated hPDCs ; however , 5 and 10 microM
PTL treatment decreased the histochemical detection and activity of
alkaline phosphatase (
ALP ) ,
alizarin red - positive mineralization , and the expression of
ALP and
osteocalcin mRNA . -
pubmed
JNK phosphorylation decreased significantly in
TNF - alpha - treated hPDCs pretreated with
PTL . -
pubmed
These results suggested that
PTL exerts negative effects on the increased osteoblastic differentiation of TNF - alpha - treated hPDCs by inhibiting
JNK signaling . -
pubmed
A facile scalable approach is presented for the rational design of multidimensional , multilayered sand -
clock - like UCNPs ( denoted as UCCKs ) bounded with high index facets , with a tunable
Nd3 + content , and without a template or multiple complicated reaction steps . -
pubmed
Graphical abstract We developed novel multidimensional multilayered sand -
clock - like upconversion nanostructures composed of a spherical inner core (
NaYF4 :
Yb ,
Er ,
Ca ) , hexagonal intermediate shell (
NaYF4 :
Yb ,
Ca ) and two up - down outer shell (
NaNdF4 :
Yb ,
Ca ) with controllable
Nd3 + as an efficient and safe probe for bioimaging applications without any quenching effect . -
pubmed
Further molecular mechanism investigation showed that
LOX inhibited the
Fas /
FasL and
p53 pathways . -
pubmed
We show that reducing glycolysis both lowers steady - state levels of
ATP and stabilizes
Smoothened (
Smo ) , the 7 - pass transmembrane protein that transduces the Hh signal . -
pubmed
We show that glycolysis is required to maintain the plasma membrane potential and that plasma membrane depolarization blocks cellular uptake of
N - acylethanolamides - lipoprotein - borne Hh pathway inhibitors required for
Smo destabilization . -
pubmed
Among the 67
cefoxitin resistant
strains , 30(44.78 % )
AmpC beta - lactamase producers were detected by MTDT and 59(88.06 % ) were detected by PCR . -
pubmed
The dominant genotype found was
CIT ( 62.69 % ) followed by
DHA ( 53.73 % ) . -
pubmed
The anti - androgenic property depends on the
androgen receptor (
AR ) nuclear translocation (
AR trafficking and its subnuclear distribution ) , the inhibition of
5alpha - reductase activity ( it possesses higher activity compared to other available
progestins ) , and the increase on
sexual hormone binding globulin (
SHBG ) levels if combined with an estrogenic counterpart . -
pubmed
Polyguanylate- and
poly ( 2 '- O - methyl ) uridylate - Sepharose have been prepared for affinity chromatography of
DNA polymerases of viral origin (
reverse transcriptase ) . -
pubmed
Both
cellular DNA polymerases and
reverse transcriptase bind to
polyguanylate - Sepharose . -
pubmed
The cellular
polymerases can be eluted from the column between 0.32 and 0.42 M
NaCl while
reverse transcriptase eluted between 0.56 and 0.78 M
NaCl . -
pubmed
However , only
reverse transcriptase adheres to
poly ( 2 '- O - methyl ) uridylate - Sepharose and can be eluted at approximately 0.35 M
NaCl . -
pubmed
[ Epidemiological and clinical significance of long - term carrier state of the Australia
antigen (
HB - Ag ) ] . -
pubmed
Polypeptide segments connecting
Cys 46 to
Cys 63 in
HiPIP and
Cys 18 to
Cys 35 in
ferredoxin are analogous in the sense that they are the same length , they connect equivalent
cysteinyl sulfur atoms , and they have similar , twisted antiparalled beta conformations . -
pubmed
Tyrosine residues 19 ( HiPIP ) and 2 (
ferredoxin ) are analogous in the sense that they interact closely with equivalent inorganic
sulfur atoms . -
pubmed
Potent inhibition of
chloramphenicol acetyl transferase (
CAT ) by
Triton X-100 and
Nonidet P-40 was observed . -
pubmed
The
CAT activity was also moderately inhibited by
sodium deoxycholate and
sodium dodecyl sulfate , and least by
Tween 20 . -
pubmed
A remarkable reduction in plasma levels of
FSH ( P less than 0.001 ) ,
LH ( P less than 0.05 , P less than 0.01 ) ,
PRL ( P less than 0.05 , P less than 0.001 ) and T ( P less than 0.001 ) along with significant diminution in the activities of testicular
delta 5 - 3 beta - HSD ( P less than 0.001 ) and
17 beta - HSD ( P less than 0.001 ) were observed following
lithium treatment for 14 and 21 days . -
pubmed
LCNA was decreased after
lithium chloride treatment for 14 and 21 days ( P less than 0.001 ) . -
pubmed
Purified
fibrinogen Ledyard had a prolonged polymerization , which was somewhat corrected by addition of
Ca2 + ions . -
pubmed
Steady - state kinetic parameters were evaluated for release of
FPA by
thrombin . -
pubmed
When the concentration of
fibrinogen Ledyard was corrected to 50 % of
total protein , because only 50 % of
fibrinogen Ledyard can release
FPA , the kinetic constants were similar to those of control
fibrinogen ( Km = 7.5 mumol / L for
A alpha chain , kcat = 54 s-1 ) . -
pubmed
The sequence of the
amino terminus of
A alpha chain showed that
Arg in position 16 was replaced by
Cys in the abnormal molecules . -
pubmed
This abnormal
fibrinogen supports
adenosine diphosphate - induced
platelet aggregation in a normal manner . -
pubmed
The studies presented herein define time - dependent modifications of
LDL constituents caused by
CuSO4 - catalyzed oxidation . -
pubmed
Measurement of the
cholesterol content of oxidized
LDL by the
cholesterol esterase - oxidase assay was found to be inaccurate . -
pubmed
Macrophage modification of
LDL also resulted in the generation of
oxysterols . -
pubmed
Since incubation of macrophages with oxidized
LDL but not native
LDL resulted in the accumulation of
oxysterols , this could account for some of the toxic and metabolic effects of oxidized
LDL on cells . -
pubmed
The
3H - product was isolated by thin - layer chromatography , and
1 - radyl-2 [ 3H ] , 3 - diacetylglycerols were prepared by
phospholipase C digestion and subsequent acetylation at the sn-3 position . -
pubmed
( 1 ) SPECULATION : Low levels of
auxin activate
cellulase ( by inducing H(+)-secretion ? ) -
pubmed
( 2 ) SPECULATION : Moderate levels of
auxin activate
cellulase further ; the
enzyme generates small amounts ( nM ) of
oligosaccharides , some of which are growth - inhibitory . -
pubmed
( 3 ) SPECULATION : Higher levels of
auxin induce
cellulase synthesis leading to the generation of higher concentrations of
oligosaccharides that can mimic
auxin . -
pubmed
( 4 ) SPECULATION : Very high levels of
auxin induce excessive levels of
cellulase , and therefore of
oligosaccharides . -
pubmed
The following parameters were measured daily : pH , pO2 , pCO2 ,
HCO3 ,
glucose ,
lactic acid ,
lactic dehydrogenase , cellular
ATP and
platelet aggregation induced by different agents (
collagen and
ADP ) . -
pubmed
A marked increase in
pO2 ,
conjugated dienes ,
malonyldialdehyde ,
lactic acid and
lactic dehydrogenase activity was observed during the preservation . -
pubmed
pCO2 ,
cyclooxygenase activity ,
vitamin E ,
coenzyme Q10 ,
bicarbonate and
glucose showed a rapid fall . -
pubmed
Fractionation of this high salt extract by
sucrose density gradient centrifugation yielded a single peak of
ATPase activity with an apparent sedimentation coefficient of 19 S. Electrophoretic analysis showed that this 19 S particle was composed of two heavy chains ( termed alpha and beta ; Mr 430,000 and 415,000 , respectively ) , five intermediate molecular weight chains (
IC1 - IC5 ; Mr 85,000 , 73,000 , 65,000 , 63,000 , and 57,000 ) , and six light chains (
LC1 - LC6 ; Mr 22,000 - 6,000 ) . -
pubmed
The 19 S particle had a specific
MgATPase activity of 1.1 + /- 0.3 mumol of
phosphate released / min / mg and exhibited an apparent Km for MgATP2- of 40 + /- 16 microM. -
pubmed
The
Mg - ATPase activity was inhibited by
vanadate , but not by
ouabain or
oligomycin , and exhibited a high activity between pH 7.0 and 10.0 with a maximum at pH 9.0 - 9.5 . -
pubmed
ATP was the preferred
nucleotide , although
GTP and
CTP ( but not
ITP ) did interact with the
dynein to a minor extent . -
pubmed
The
tricarboxylic acid cycle , glycolysis , gluconeogenesis , hepatic ketogenesis , and metabolism of
ethanol are some of the metabolic processes amenable to
13C MRS . -
pubmed
Carbon-13 MRS offers potential as a new investigative probe into organ physiology . -
pubmed
The lowest effects in the cell - mediated experiments were seen with 7- , 8- , 9- and 10 -
MBP ,
2 - MBA , and
3 - MBA . -
pubmed
On the other hand , among all the compounds tested
7,12 - dimethylbenz ( a ) anthracene ,
12 - MBA , and 11 -
MBP , which have the
methyl group attached in the bay - region position opposite the terminal
benzo ring , showed the highest mutagenic efficacies in the cell - mediated test , as compared to those observed in the homogenate - mediated test . -
pubmed
When tested for melanophore pigment - dispersing activity in destalked Uca ,
[ Glu3 ] alpha - PDH was found to be 325 % more potent than
alpha - PDH . -
pubmed
Binding affinities were measured by the competitive displacement by
bile acids of
1 - anilino-8 - naphthalene sulfonate (
ANS ) bound to the
proteins and , in some cases , by direct methods of flow dialysis and equilibrium dialysis . -
pubmed
Studies with specifically labeled
( 2H ) NAD ( P ) H and
2H2O and of the
enzymes involved . -
pubmed
Dihydrofolate reductase catalyzed the transfer of the pro - R
hydrogen of
NAD ( P ) H during the reduction of
7,8 - dihydrobiopterin to
tetrahydrobiopterin . -
pubmed
Sepiapterin reductase catalyzed the transfer of the pro - S
hydrogen of
NADPH during the reduction of
sepiapterin to
7,8 - dihydrobiopterin . -
pubmed
These results suggest that
dihydrofolate reductase is not involved in the biosynthesis of
tetrahydrobiopterin from
dihydroneopterin triphosphate . -
pubmed
Our results suggest that the reduction of
6 - pyruvoyl - tetrahydropterin might be catalyzed by
sepiapterin reductase . -
pubmed
Proceedings : Effects of
phenobarbitone on
glucose transport and membrane
ATP - ase activities of cerebral cortex . -
pubmed
35
C1 NMR studies of the active site
zinc of
horse liver alcohol dehydrogenase . -
pubmed
Embryonic chick intestine in organ culture : interaction of
adenylate cyclase system and
vitamin D3 - mediated
calcium absorptive mechanism . -
pubmed
Protein kinase and
cyclic 3 ' , 5 '- AMP : significance of binding and activation constants . -
pubmed
Ribonucleotides and
ribonucleosides as
methyl acceptors for
S - adenosylmethionine : ( amino - and thio -) purine methyl - transferases . -
pubmed
In contrast ,
protamine sulfate enhanced the specific binding of 125I -
EGF by increasing the apparent number of
EGF receptors on the cell surface . -
pubmed
The increase in 125I -
EGF receptor binding was not prevented by
cycloheximide nor by
actinomycin D. -
pubmed
Protamine
II ( MW approximately 4,800 daltons ) was equally active ( half maximum inhibition concentration approximately 0.4 microM ) ;
protamine IV ( MW approximately 3,300 daltons ) was substantially less active ( half maximum inhibition concentration approximately 2.8 microM ) . -
pubmed
These investigations have extended previous observations that
protamine sulfate is a potent inhibitor of
PDGF binding and establish that
protamine sulfate blocks
PDGF binding at the physiological receptor , preventing
PDGF initiated biological activities . -
pubmed
The activity of the processing
protease in purified brush border membranes was examined using endogenous substrates labeled with
[ 3H ] fucose and
[ 35S ] methionine . -
pubmed
This fraction is enriched 5 - fold in the plasma membrane marker enzymes
adenyl cyclase (
EC 4.6.1.1 ) and
ouabain - inhibitable , (
Na + + K +)- dependent adenosine triphosphatase (
EC 3.6.1.3 ) . -
pubmed
It is also enriched up to 14 - fold in iodinated surface proteins , and up to 4 - fold in (
3H - mannose - labeled
glycoproteins , of which the major variable surface coat glycoprotein is the main constituent . -
pubmed
Several high molecular weight surface
glycopeptides have been selectively investigated and partially characterized by a combination of metabolic labeling with
[ 3H ] mannose ,
lactoperoxidase - catalyzed surface iodination , and affinity chromatography on Con A - Sepharose . -
pubmed
Immunochemical identification of normal and variant
hemoglobins after electrophoretic separation and transfer to
nitrocellulose membranes . -
pubmed
Renal excretion of
calcium may have been impaired by
renal dysfunction resulting in
hypercalcaemia and suppression of plasma
PTH and
1,25 ( OH ) 2D3 levels . -
pubmed
Serum
calcium and serum
phosphorus were reduced 3 h after the daily subcutaneous
CT injection ( 3 MRC - U / kg
body weight ) , whereas a rebound increase in the serum levels of both minerals was observed at 24 hours after the injection . -
pubmed
Following an early transient increase , the incorporation of
strontium-85 into the fractured bones was impaired after 30 days of
CT treatment . -
pubmed
The effects of
aspirin on
cataract formation may result from 1 ) lowering of plasma
tryptophan levels and increased excretion of
tryptophan metabolites , 2 ) inhibition of
aldose reductase and
sorbitol formation in the
diabetic lens , 3 ) inhibition of
tryptophan or
kynurenine binding to lens protein . -
pubmed
Effects of targeted
neuronal nitric oxide synthase gene disruption and
nitroG - L - arginine methylester on the threshold for
isoflurane anesthesia . -
pubmed
Nonselective inhibition of
nitric oxide synthase by
nitroG - L - arginine methylester (
L - NAME ) reduces the minimum alveolar concentration of
halothane anesthesia . -
pubmed
Closure of the first spinal reflex are is established within 2 days and follows an antidromic pattern as related to the physiological spread of nerve impulses : synapses on motoneuronal
somata and primary dendrites in the basal plate appear first and are followed by synapses in the
alar plate , between dorsal root axon collaterals and
somata of borderline ( commissural / association ) neurons . -
pubmed
In batch fermentation studies ,
aad expression restored
butanol formation ( 84 mM ) in mutant M5 without any
acetone formation or any significant increase in
ethanol production . -
pubmed
The results demonstrate that in this case three doses of
piperacillin have a higher efficacy than one dose in the prevention of
febrile complications and
post - operative
infections . -
pubmed
The antiaversive effects of
morphine result from its action on
mu receptors and the aversive ones may be associated with its action on other
opiate receptors . -
pubmed
RESULTS :
IL-1 beta had no immediate effect on the basal or stimulated release of
ACh . -
pubmed
A marked suppression of
ACh release was observed in tissues that had been preincubated with
IL-1 beta for 60 minutes or more . -
pubmed
The
IL-1 beta effect was also blocked by
cycloheximide and was spontaneously reversible after 60 minutes . -
pubmed
CONCLUSION : It was concluded that
IL-1 beta suppresses
ACh release via the formation and release of a protein mediator that could be another
cytokine , including
IL-1 . -
pubmed
BACKGROUND : We have recently described the association between the
IgE antibody response to
Ole e I ( the major
antigen from olive tree pollen ) and the
DR7 - DQ2 haplotype in a Spanish population . -
pubmed
OBJECTIVE AND METHODS : Due to the linkage disequilibrium between
DR7 and
DQ2 , and thus the difficult distinction between the role of these two
antigens in the T - cell activation response , we decided to solve this question by two approaches : 1 . -
pubmed
Bronchial responsiveness to
methacholine decreased significantly only after 6 wk of treatment with
FP ( p & lt ; 0.05 ) . -
pubmed
The effects on
cholesterol were the result of a decrease in LDL- and
VLDL -
cholesterol without any modification of HDL -
cholesterol . -
pubmed
A synthetic transposon was constructed containing an ori , an
ampicillin resistance ( Ampr ) gene , a multi - cloning site (
MCS ) and two hyperactive end sequences . -
pubmed
The
NOS antagonist ,
nitro - L - arginine ( 300 microM ) inhibited neurogenic relaxations in control and extrinsically - denervated tissues . -
pubmed
Capsaicin- but not
6 - OHDA - treatment mimicked the effects of extrinsic denervation on
NOS - ir and neurogenic relaxations of the longitudinal muscle . -
pubmed
Troglitazone ( 1 - 20 microM ) dose - dependently inhibited both spontaneous and
insulin - stimulated
ET-1 secretion . -
pubmed
This inhibitory effect of
troglitazone was associated with reduced
ET-1 mRNA levels . -
pubmed
Addition of
indomethacin ( 100 microM ) or
Nw - nitro - l - arginine methyl ester ( 1 mM ) and downregulation of
protein kinase C by prolonged pretreatment of the cells with a
phorbol ester ,
12 - O - tetradecanoylphorbol 13 - acetate , did not affect the inhibitory effect of
troglitazone at concentrations up to 10 microM. -
pubmed
Other
PPARgamma ligands ,
pioglitazone ( 1 - 10 microM ) and
15 - deoxy - delta 12 , 14 - prostaglandin J2 ( 1 - 10 microM ) , but not a
PPARalpha ligand ,
bezafibrate ( 1 - 10 microM ) , dose - dependently suppressed spontaneous
ET-1 secretion from bVECs . -
pubmed
These results , taken together , suggest that
troglitazone inhibits
ET-1 mRNA expression and secretion in bVECs possibly through activation of
PPARgamma . -
pubmed
This inhibition may contribute to the
hypotensive effect of
troglitazone in
insulin - resistant subjects . -
pubmed
A synthetic peptide of 20
amino acids from the putative binding site competed with in vitro synthesized
NSP1 for binding to liposomes containing
phosphatidylserine . -
pubmed
DCM was identified by headspace gas chromatography - mass spectrometry (
GC -
MS ) . -
pubmed
Interestingly , coadministration of the vasodilator and the
nitric oxide (
NO ) donor
nitroglycerin not only prevented the increase of
CPP and LVEDP , but also uncovered a slight positive inotropic effect of
ET-1 in
LVH hearts . -
pubmed
Physical interactions between
phospholamban and
sarco ( endo ) plasmic reticulum Ca2 + - ATPases are dissociated by elevated
Ca2 + , but not by
phospholamban phosphorylation ,
vanadate , or
thapsigargin , and are enhanced by
ATP . -
pubmed
Because the 1D11 antibody relieves inhibitory interaction between the two proteins , it was of interest to determine whether
PLN phosphorylation or elevation of
Ca ( 2 + ) , which also relieves inhibitory interactions between
PLN and
SERCA , would disrupt physical interactions . -
pubmed
Co - immunoprecipitation was measured in the presence of increasing concentrations of
Ca ( 2 + ) or after phosphorylation of
PLN by
protein kinase A. -
pubmed
Physical interactions were dissociated by elevated
Ca ( 2 + ) but not by
PLN phosphorylation . -
pubmed
The addition of
ATP enhanced interactions between
PLN and
SERCA . -
pubmed
The further addition of
vanadate and
thapsigargin , both of which stabilize the E(2 ) conformation , did not diminish binding of
PLN to
SERCA . -
pubmed
These data suggest that physical interactions between
PLN and
SERCA are stable when
SERCA is in the
Ca ( 2 +)-free E(2 ) conformation but not when it is in the E(1 ) conformation and that phosphorylation of
PLN does not dissociate physical interactions between
PLN and
SERCA . -
pubmed
Even though the
ubiquitin cross - reactivity was transiently lost from the sperm mitochondria during epididymal passage , likely as a result of
disulfide bond cross - linking , it was restored and amplified after fertilization . -
pubmed
Serial blood samples were collected 24 h
post - dose to measure
fluticasone propionate plasma concentrations . -
pubmed
Similar or lower
fluticasone propionate systemic exposure was observed with the
HFA 134a pMDIs compared to the corresponding
CFC inhalers . -
pubmed
Therefore , the
fluticasone propionate HFA 134a pMDI is an acceptable pharmaceutical alternative to the current
CFC pMDI , producing similar
lung deposition and no increase in systemic exposure at microgram equivalent doses . -
pubmed
During the chronic phase of
inflammation , in either model ,
CP reduced the elevated serum and exudate ( local )
LTB ( 4 ) and
IL-6 levels . -
pubmed
The antiarthritic effect of
CP was also accompanied by augmentation of serum level of protein
thiols , with reduction or normalization of elevated systemic and/or local levels of lipid
peroxide ,
superoxide dismutase , and
glutathione . -
pubmed
The presence of
erm genes coding for
ribosomal methylases and of
mefE - like genes responsible for
macrolide efflux was screened by a multiplex
polymerase chain reaction and confirmed by DNA / DNA hybridization . -
pubmed
Of the 110
strains studied , 108 were cross - resistant to
erythromycin ,
spiramycin and
clindamycin , including 105
strains containing
ermB - related genes and three
strains that contained a combination of
ermB- and
mefE - related genes . -
pubmed
Two
strains apparently susceptible to
clindamycin but resistant to
spiramycin also contained
ermB - related genes . -
pubmed
No
strain was resistant to
erythromycin alone or contained only a
mef - like gene . -
pubmed
In this study , we found that
Dex inhibited both spontaneous and
TNF - induced activation of
cPLA ( 2 ) . -
pubmed
Dex had no direct effect on cellular
cPLA ( 2 ) levels , but facilitated
cPLA ( 2 ) degradation upon subsequent stimulation of cells with
TNF . -
pubmed
In addition ,
Dex increased synthesis of the endogenous
cPLA ( 2 ) inhibitor lipocortin 1 (
LC1 ) . -
pubmed
An antisense oligonucleotide to
LC1 could completely abrogate
Dex - induced resistance to the cytotoxic action of
TNF . -
pubmed
Compared with the control group ,
MB reduced the requirement for
norepinephrine ,
epinephrine , and
dopamine by as much as 87 % , 81 % , and 40 % , respectively . -
pubmed
MB also reduced the body temperature and the plasma concentration of
nitrates /
nitrites . -
pubmed
Leukocytes and organ function variables such as
bilirubin ,
alanine aminotransferase ,
urea , and
creatinine were not significantly affected . -
pubmed
Results from this study indicate that the
monoclonal antibody - based ELISA could be useful for a sensitive , quantitative , or qualitative
ractopamine screening assay . -
pubmed
Regulation of
ryanodine receptors via macromolecular complexes : a novel role for
leucine /
isoleucine zippers . -
pubmed
The mechanism underlying this loss of cardiac EC coupling gain involves altered regulation of the cardiac
ryanodine receptor (
RyR2 ) , the major sarcoplasmic reticulum
Ca ( 2 + ) release channel in the heart . -
pubmed
The targeting of
PKA ,
PP1 , and
PP2A to
RyR2 is dependent on the binding of targeting proteins to the channel via highly conserved
leucine /
isoleucine zippers ( LIZs ) . -
pubmed
We report for the first time the
mucicarmine - staining pattern of this
tumor as well as immunohistochemistry directed against
cytokeratin 7 , the
estrogen receptor , and the
progesterone receptor . -
pubmed
These include
argyrophilia , as detected by
silver degeneration stains , and an elevation in
glial fibrillary acidic protein (
GFAP ) , a marker of reactive
gliosis in response to
injury , as well as a long - term decrease in
tyrosine hydroxylase (
TH ) protein levels . -
pubmed
The
SK3 subunit of small conductance
Ca2 + -activated
K + channels interacts with both
SK1 and
SK2 subunits in a heterologous expression system . -
pubmed
When
rSK3VK was co - expressed with
rSK2 the resulting currents had an intermediate sensitivity to
UCL 1848 ( IC50 of approximately 5 nM compared with 120 pM for
rSK2 and & gt;300 nM for
rSK3VK ) , suggesting that
rSK3 and
rSK2 can form functional heteromeric channels . -
pubmed
To determine whether these assemblies were functional we created
rSK3VF , an
rSK3 mutant with an enhanced affinity for
tetraethylammonium chloride (
TEA ) ( IC50 of 0.3 mM ) . -
pubmed
Co - transfection of
rSK3VF and
hSK1 produced currents with a sensitivity to
TEA not different from that of
hSK1 alone ( IC50 approximately 15 mM ) . -
pubmed
At meiotic prophase , the meiocyte walls were additionally marked by the appearance of poorly
methyl - esterified domains of homogalacturonan and of ( 1 - ->4)-
beta - Gal residues , detected by
JIM5 and LM5 . -
pubmed
PURPOSE :
RSR13 , an allosteric modifier of
hemoglobin , reduces
hemoglobin -
oxygen binding affinity facilitating
oxygen release from
hemoglobin , resulting in increases in tissue pO(2 ) . -
pubmed
The purpose of this study was noninvasively to monitor the time course and effect of
RSR13 on
tumor oxygenation , directly using in vivo electron paramagnetic resonance (
EPR oximetry ) , and indirectly using blood
oxygen level dependent magnetic resonance imaging ( BOLD MRI ) . -
pubmed
Then , for 6 consecutive days and after an initial baseline
EPR measurement ,
RSR13 ( 150 mg / kg ) or vehicle ( same volume ) was injected intraperitoneally , and measurements of intratumoral
oxygen were made at 10 - min intervals for the next 60 min . -
pubmed
RESULTS : Based on
EPR measurements ,
RSR13 produced statistically significant temporal increases in
tumor pO(2 ) over the 60 - min time course , which reached a maximum at 35 - 43 min postdose . -
pubmed
There was little change in R(2 ) * , indicating that the
RSR13 had minimal detectable effects on total deoxyhemoglobin and
hemoglobin -
oxygen saturation . -
pubmed
The dynamics of
tumor pO(2 ) after
RSR13 administration may be useful for the design of clinical protocols using allosteric
hemoglobin effectors . -
pubmed
Use of
fatty acid RPMI 1640 media for testing susceptibilities of eight Malassezia species to the new
triazole posaconazole and to six established antifungal agents by a modified NCCLS M27 -
A2 microdilution method and Etest . -
pubmed
dermatis ( 2 isolates ) against
amphotericin B ,
ketoconazole ,
itraconazole ,
fluconazole ,
voriconazole ,
terbinafine , and
posaconazole by
BMD and Etest . -
pubmed
Because of higher
amphotericin B MICs provided by Etest for
strains also displaying high
BMD MICs ( & gt;/=1 microg / ml ) , agreement was poorer . -
pubmed
The changes in the
rest energy expenditure ( REE ) , non - protein respiratory quotient ( NPRQ ) ,
glucose metabolism ,
nitrogen balance as well as plasma levels of endocrines and inflammatory mediators were determined before and after the administration of
rhGH . -
pubmed
The plasma levels of
PGE2 ,
TXB2 ,
TNF - alpha and
IL-6 significantly decreased and
insulin ,
rhGH and IGF-1 obviously increased when compared with those in group B. -
pubmed
Tumor size , histology , grade , lymph node status ,
estrogen (
ER ) and
progesterone receptor (
PgR ) status ,
HER-2 expression , peritumoral vascular invasion ( PVI ) ,
Ki-67 expression , extensive in situ component ( EIC ) , and multifocality between the two groups were analyzed . -
pubmed
Heat - induced gelation ( 80 degrees C , 30 min or 85 degrees C , 60 min ) of whey protein concentrate ( WPC ) solutions was studied using transmission electron microscopy (
TEM ) , dynamic rheology and
polyacrylamide gel electrophoresis ( PAGE ) . -
pubmed
TEM analyses showed that the
SDS - gels had finer aggregate structure ( approximately equal to 10 nm ) than the control gels ( approximately equal to 100 nm ) , whereas the
DTT - gels had a more particulate structure ( approximately equal to 200 to 300 nm ) . -
pubmed
In both the
SDS - solutions and the
DTT - solutions , the denatured whey
protein molecules were in the form of monomers or small aggregates . -
pubmed
Results indicated that the mixture of MC /
CP performed well as a biofilter media and the
ammonia removal efficiency was 100 % for the filter depth of 500 mm . -
pubmed
Asymmetric dimethylarginine : a molecule responsible for the coexistence of
insulin resistance and
atherosclerosis via dual
nitric oxide synthase inhibition . -
pubmed
Asymmetric dimethylarginine (
ADMA ) has been recently identified as the major endogenous inhibitor of soluble
nitric oxide synthase . -
pubmed
Here , we propose that
ADMA causes
insulin resistance by the inhibition of the neuronal isoform of
nitric oxide synthase , while the simultaneously observed
atherosclerosis is the consequence of
endothelial nitric oxide synthase (
NOS ) inhibition . -
pubmed
In these models , loss of hepatic
nitric oxide productions is presumed to hinder a very potent
insulin sensitizing mechanism referred to as meal induced sensitization that is anatomically linked to the nitrergic fibers of the anterior hepatic plexus . -
pubmed
In our hypothesis , we suggest that
ADMA by inhibiting both the neuronal and the endothelial forms of
NOS , results both in
insulin resistance and in accelerated
atherosclerosis , therefore
ADMA is the molecule responsible for the coexistence of these two conditions . -
pubmed
HIF-1 , a
hypoxia inducible transcription factor , plays a pivotal role in the cellular response to
hypoxia by activating genes involved in
glucose metabolism ,
vascular remodeling , and erythropoiesis . -
pubmed
Further studies revealed that small molecule inhibitors of
p38 (
SB203580 ) and
MEK /
ERK (
U1026 ) further reduced the remaining effect of
TGF - beta1 in
Smad3 - deficient fibroblasts . -
pubmed
To move from finger to ear concentrations , using the
Lactate Pro , modelling analysis suggested a regression equation of Y = 0.827x + 0.769 adjustment for pre climb samples and Y = 0.955x + 0.566 for
post climb concentrations . -
pubmed
Under conditions of
oxygen and
glucose deprivation , translocation rates of both compounds increased ; however , unlike rtPA , desmoteplase transport remained
LRP - dependent . -
pubmed
The replacement of the
amino acid residue
A43 , located in the second shell of
riboflavin synthase active center , in the recombinant N - terminal domain dimer reduces the affinity for
6,7 - dimethyl-8 - ribityllumazine . -
pubmed
The mutation of the
amino acid residue
C48 forming part of activity cavity of the
enzyme causes significant
( 19 ) F NMR chemical shift modulation of
trifluoromethyl derivatives of
6,7 - dimethyl-8 - ribityllumazine in complex with the
protein , while substitution of
A43 results in smaller chemical shift changes . -
pubmed
Observations on the influence of exogenous
gonadotrophins and
cloprostenol on ovulation in gilts . -
pubmed
We studied the effects of
gonadotrophins and
prostaglandin ( PG ) F ( 2alpha ) on ovulation in gilts . -
pubmed
At 34 and 36 h after
hCG , gilts received injections of either 500 mug or 175 mug
PGF ( 2alpha ) (
cloprostenol ) , or had no injections . -
pubmed
Laparotomies were performed at 36 h (
cloprostenol gilts ) or 38 h ( controls ) after
hCG injection . -
pubmed
AIM : To investigate the hypothesis that the protective effects of
curcumin in hepatic warm
ischemia /
reperfusion ( I / R ) injury are associated with increasing
heat shock protein 70 (
Hsp70 ) expression and antioxidant enzyme activity . -
pubmed
A higher rate of apoptosis was observed in I / R group than in C + I / R group , and a significant increase of
MDA ,
NO ( 2 )(- ) +
NO ( 3 )(- ) and
MPO level in liver tissues and
serum transaminase concentration was also observed in I / R group compared to C + I / R group . -
pubmed
Curcumin also decreased the activity of
inducible NO synthase (
iNOS ) in liver after reperfusion , but had no effect on the level of
endothelial NO synthase (
eNOS ) after reperfusion in liver . -
pubmed
Dermal
calcium loss during exercise was estimated using a patch collection technique to examine
calcium loss as a potential mediator of changes in
BMD . -
pubmed
There were no differences in changes in
BMD between the
calcium supplementation groups . -
pubmed
Higher dermal
calcium losses were associated with lower baseline
BMD values at the total hip , neck , and shaft ( all p & lt ; 0.05 ) , but were not significantly associated with changes in
BMD . -
pubmed
For instance ,
endonuclease III (
Nth ) does not excise
thymine glycol ( Tg ) when it is part of either tandem lesion . -
pubmed
The tandem lesion is also incised by the bacterial
nucleotide excision repair system
UvrABC with almost the same efficiency as an isolated Tg . -
pubmed
Rapid and sensitive HPLC -
MS /
MS method for pharmacokinetic assessment of
ribavirin in healthy Chinese . -
pubmed
Colloidal gold nanoparticles ( AuNPs ) were directly assembled onto a surface of
SPR Au chip via
2 - aminoethanethiol for the enhancement of sensitivity as a label - free detection system . -
pubmed
SPR analyses indicated the signal increases for successive binding of
hIgG and
anti - hIgG onto the
GBP -
ProA layered AuNPs - assembled chip were higher ( about 92 and 30 % , respectively ) than that onto the identically treated bare chip . -
pubmed
These results demonstrate the direct assembly of AuNPs onto a
SPR chip could enhance the signal in biomolecular interaction events , and the
GBP -
ProA protein could be a valuable crosslinker for simple and oriented immobilization of antibodies onto
Au chip surfaces without any surface chemical modification . -
pubmed
Further , we analysed
LeExt1::
beta - glucuronidase reporter gene for root hair formation and found increasing expression of
LeExt1 in the
CO - exposed root hairs . -
pubmed
Several protein growth factors can induce endothelial cell proliferation and angiogenesis , through signal transduction cascades that result in the production of several inflammatory mediators and lipid second messengers such as
prostaglandins and
Platelet Activating Factor (
PAF ) . -
pubmed
S - Nitrosothiols (
RSNOs ) represent an important class of
post - translational modifications that preserve and amplify the actions of
nitric oxide and regulate enzyme activity . -
pubmed
Herein , we report the reaction of
S - nitrosated cysteine ,
glutathione , and a mutated C165S
alkyl hydroperoxide reductase with the water - soluble
phosphine tris ( 4,6 - dimethyl-3 - sulfonatophenyl ) phosphine trisodium salt hydrate (
TXPTS ) . -
pubmed
A combination of NMR and
MS techniques reveals that these reactions produce covalent
S - alkylphosphonium ion adducts ( with
S - P (+ ) connectivity ) ,
TXPTS oxide , and a
TXPTS - derived
aza - ylide . -
pubmed
In conclusion , the present work reveals an unexpected function of
dMyc in the systemic control of growth in response to
steroid hormone signaling . -
pubmed
In diestrus , with the rise of
progesterone , four ( I -
IV ) distinct morphological changes were evident . -
pubmed
Mortality , serum levels of markers of
hepatorenal injuries ,
cytokines ,
insulin ,
glucose and lipid derivatives , and the hepatic signaling pathway regulating gluconeogenesis were investigated . -
pubmed
Consistently , apart from the case of
3 ( 3 + ) , which shows some degree of delocalization , all of the oxidized forms of 3(2 + ) , 4(2 + ) , and 5(2 + ) can be described as class
II , localized mixed - valent species , in agreement with the electrochemical data showing two close oxidation potentials around 1 V vs
FeCp *( 2 ) . -
pubmed
Atorvastatin is a selective
HMG - CoA reductase competitive inhibitor , used for the treatment of
hyperlipidaemia . -
pubmed
Hence there is more possibility of
atorvastatin for inhibition of metabolism of
glyburide , by both
CYP 2C9 and
3A4 . -
pubmed
The results of this study revealed that
atorvastatin led to the PK / PD changes have been due to
glyburide increased bioavailability , decrease volume of distribution , and/or decrease total clearance may be due to the inhibition of
cytochrome P450 metobolic system . -
pubmed
The studied subjects were determined in plasma : lipid parameters ( T - C ,
HDL - C ,
TG ,
LDL - C ) , total antioxidant status , TAS ( Randox , Statfax 1904 Plus ) and
thiobarbituric acid - reactive substances , TBARS ( Sigma , UV-160A Shimadzu ) as
lipid peroxidation products . -
pubmed
NGT - P group comparing with NGT - N group presented higher concentration of fasting
glucose , T - C ,
LDL - C , and TBARS ( 8.43 + /- 5.24 vs 4.81 + /- 2.58 nmol/ l ) . -
pubmed
Media were analyzed for ovarian
steroids and anti - mullerian hormone (
AMH ) . -
pubmed
In the presence of
fetuin at 5 %
O2 , follicles had the highest survival rate if cultured with high or medium
FSH , whereas follicles grew to larger diameters at Week 5 in low
FSH . -
pubmed
High
FSH stimulated
steroid production by growing follicles , and steroidogenesis by follicles cultured with low
FSH was promoted by
LH . -
pubmed
AMH biosynthesis was elevated with high compared with low
FSH and for longer under 5 %
O2 than under 20 %
O2 . -
pubmed
Although the
CFP signal was equally distributed in both cells of a germinating conidium , reactive
oxygen species accumulated only in the cell that produced a germ tube , indicating that
CgRac1 was active only in the germinating cell . -
pubmed
Here , we demonstrate the efficacy of a strong base ,
1,8 - bis ( dimethylamino ) naphthalene (
DMAN ; proton sponge ) , as a novel matrix for MALDI - time - of - flight (
TOF ) MS analysis of whole cell bacteria . -
pubmed
These results indicate that
DMAN represents a suitable matrix for MALDI -
TOF MS analysis of mixtures of complex lipids as the intact membranes of microorganisms . -
pubmed
The tensor components were determined by analyzing the configuration of
phalloidin stained
F - actin fibers in the media layer of pulmonary arteries . -
pubmed
[ Effect of local
basic fibroblast growth factor and
5 - fluorouracil on accelerating healing and preventing tendon adhesion after flexor tendon repair ] . -
pubmed
CONCLUSION : Local single - use
bFGF and
5 - FU can not only effectively promote the healing of flexor tendon , but also significantly reduce tendon adhesion . -
pubmed
Relation of sex and
estrogen therapy to
serum fibroblast growth factor 23 , serum
phosphorus , and urine
phosphorus : the Heart and Soul Study . -
pubmed
Fibroblast growth factor 23 (
FGF-23 ) is a hormone originating from bone that regulates urine
phosphorus excretion . -
pubmed
Whether sex or
estrogen therapy is associated with different
FGF-23 levels is unknown . -
pubmed
OUTCOMES : Serum
phosphorus , tubular maximum reabsorption of
phosphorus indexed to
glomerular filtration rate ( TMP /
GFR ) , and plasma
FGF-23 concentrations . -
pubmed
Mean
FGF-23 levels were 68.7 ( 95 % CI , 59.7 - 79.0 ) relative units ( RU)/mL in non -
estrogen - using women , 43.8 ( 95 % CI , 41.2 - 46.5 ) RU / mL in men , and 45.1 ( 95 % CI , 35.2 - 57.4 ) RU / mL in women using
estrogen in adjusted analysis ( P & lt ; 0.001 ) . -
pubmed
In the context of prior literature , these data suggest that postmenopausal
phosphorus retention may stimulate higher
FGF-23 concentrations after menopause . -
pubmed
The screen included height , weight , and systolic (
SBP ) and diastolic (
DBP ) blood pressure measurements plus a blood draw following an overnight fast for fasting
glucose . -
pubmed
INVESTIGATIONS : All approaches on the metabolic ward to control
insulin resistance failed as well and finally blood
glucose levels could only be stabilized with the aid of continous subcutaneous
insulin infusion with more than 500 units per day . -
pubmed
Heme Oxygenase-1 and
breast cancer resistance protein protect against
heme - induced toxicity . -
pubmed
We postulated that overexpression of
Heme Oxygenase-1 (
HO-1 ) and
Breast Cancer Resistance Protein (
BCRP ) would protect against
heme - induced
cytotoxicity . -
pubmed
HO-1 is a
heme - degrading enzyme generating
carbon monoxide ,
iron , and
biliverdin /
bilirubin , while
BCRP is a
heme efflux transporter . -
pubmed
Heme - induced cell
death was significantly attenuated when cells overexpressed
HO-1 ,
BCRP , or both . -
pubmed
The protective effects of
HO-1 overexpression were most pronounced , while co - treatment with the HO - activity inhibitor
tin mesoporphyrin reversed these protective effects . -
pubmed
Also cells treated with the anti - oxidants
N - acetylcysteine or
HO - effector molecule
bilirubin showed protection against
heme insults , which may explain the increased protection by
HO-1 compared to
BCRP . -
pubmed
In conclusion , both
HO-1 and
BCRP protect against
heme - induced toxicity and may thus form novel therapeutic targets for
heme - mediated pathologies . -
pubmed
DESIGN : To evaluate neurobehavioral alterations following the development of DIO induced by an 8 - week high - fat diet (
HF ) exposure , striatal
D2 - receptor density , DAT function and expression , extracellular
dopamine concentrations , impulsivity , and motivation for high- and low - fat reinforcers were determined . -
pubmed
To determine predictors of DIO , neurobehavioral antecedents including impulsivity , motivation for high - fat reinforcers , DAT function and extracellular
dopamine were evaluated before the 8 - week
HF exposure . -
pubmed
METHODS : Striatal
D2 - receptor density was determined by in vitro kinetic analysis of
[ ( 3 ) H ] raclopride binding . -
pubmed
DAT function was determined using in vitro kinetic analysis of
[ ( 3 ) H ] dopamine uptake ,
methamphetamine - evoked
[ ( 3 ) H ] dopamine overflow and no - net flux in vivo microdialysis . -
pubmed
Activity was measured by monitoring the levels of
aspartate aminotransferase (
AST ) ,
alanine aminotransferase (
ALT ) ,
alkaline phosphatase (
ALP ) and
bilirubin , along with histo - pathological analysis . -
pubmed
Herein a method is described for labeling mesenchymal stem cells (
MSC ) , in which the physical state of
citrate - coated nanoparticles ( dispersed versus aggregated ) can be kinetically tuned through electrostatic and magnetic triggers , as monitored by diffusion light scattering in the extracellular medium and by optical and electronic microscopy in cells . -
pubmed
RhoA - induced
NFAT activation and
fibronectin upregulation were both dependent on the
calcium / calmodulin pathway and
Rho kinase . -
pubmed
RhoA activation induced a rise of intracellular
calcium ion concentration ( [
Ca ( 2 +)]i ) , which was at least in part dependent on the
transient receptor potential canonical 6 (
TRPC6 ) cation channel . -
pubmed
The results indicate that
RhoA activates
NFAT by inducing a rise of [
Ca ( 2 +)]i in podocytes , which in turn contributes to
fibronectin upregulation . -
pubmed
We observed dysregulation of genes involved in bone remodeling (
osteoprotegerin ,
osteocalcin ,
osteopontin ) and
vitamin D metabolism (
7 - dehydrocholesterol reductase ,
Gc - globulin ,
Cyp2r1 ,
Cyp27a1 ) as well as alterations in
calcium and
vitamin D homeostasis . -
pubmed
AIM : In paediatric dentistry ,
epinephrine may contribute to systemic and local side - effects . -
pubmed
Interestingly ,
enkephalins and
mu - opioid receptors (
mu - ORs ) are known for their role in ingestive behaviors and have previously been shown to regulate the excitability of MnPO neurons following a single
Na (+ ) depletion . -
pubmed
Interestingly , the subpopulation of
Na (+ ) sensors responding to
D - Ala(2 ) ,
N - MePhe ( 4 ) ,
Gly - ol - enkephalin (
DAMGO ) , a specific
mu - OR agonist , represented 11 % of MnPO neurons after a single
Na (+ ) depletion and the population doubled after three
Na (+ ) depletions . -
pubmed
The best fit multiple regression model ( Adjusted
R2 : 0.494 , p = 0.020 ) included Plasma
Glucose as an independent variable , and the squared value of cohesion score ,
depression score of
HAD , Total calorie intake , Exercise time , Housekeeping time , and
BMI were dependent variables . -
pubmed
The potent
metallo - beta - lactamase NDM-1 efficiently hydrolyses all classes of
beta - lactam antibiotics (
penicillins ,
cephalosporins and
carbapenems ) and is also associated with multiple determinants that enable the bacteria to become resistant to other
antibiotic classes ( Nordmann et al . -
pubmed
In the presence of
NDM-1 other
beta - lactamases may go unobserved because of the spectrum of activity of NDM-1 against all
beta - lactam antibiotics . -
pubmed
BACKGROUND :
Angiotensin I - converting enzyme (
ACE ) has two functional N- and C - domain active centers that display differences in the metabolism of biologically - active peptides including the hemoregulatory tetrapeptide ,
Ac - SDKP , hydrolysed preferentially by the N domain active center . -
pubmed
In silico analysis demonstrated S333W localized in the
S1 pocket of the active site of the N domain with the bulky
Trp adversely affecting binding of
ACE substrates due to steric hindrance . -
pubmed
CONCLUSIONS AND SIGNIFICANCE : A novel Ser333Trp
ACE mutation results in dramatic changes in
ACE kinetic properties and lowered clearance of
Ac - SDKP . -
pubmed
Wound bed preparation 2014 update : management of critical colonization with a
gentian violet and
methylene blue absorbent antibacterial dressing and elevated levels of
matrix metalloproteases with an ovine
collagen extracellular matrix dressing . -
pubmed
Group
RS ( n = 20 ) received
USGRSB 0.5 mg kg(-1 ) 0.25 %
bupivacaine per side in the posterior rectus sheath compartment . -
pubmed
Pharmacological inhibitors of lysosomal degradation , such as
chloroquine , increased
AF1Q levels , whereas activators of CMA , including
6 - aminonicotinamide and nutrient
starvation , decreased
AF1Q levels . -
pubmed
Using an
amino acid deletion
AF1Q mutation plasmid , we identified that
AF1Q had a KFERQ - like motif which was recognized by
HSPA8 for CMA - dependent proteolysis . -
pubmed
INTRODUCTION :
Edoxaban is an oral , once - daily direct
factor Xa (
FXa ) inhibitor . -
pubmed
Intrinsic
FX activity was increased up to 20 % and 31 % of normal in the presence of
edoxaban by rFVIIa and FEIBA , respectively . -
pubmed
CONCLUSIONS : The findings of this ex vivo study suggest that rFVIIa and FEIBA rapidly reversed
edoxaban - mediated anticoagulation effects based on PT and aPTT , but had minimal effect based on intrinsic
FX activity . -
pubmed
RESULTS : The consumption of probiotic yogurt caused significant decrease in
LDL - c /
high density lipoprotein cholesterol (
HDL - c ) ratio ( 3.13 + - 1.00 - 2.07 + - 0.71 , P = 0.016 ) . -
pubmed
It was found that to achieve efficient
nitrogen removal via partial nitrification - denitrification (
PND ) , " DO elbow " point must appear at the end of the last aeration period . -
pubmed
There was a correlation between the
ammonium oxidizing bacteria ( AOB)/
nitrite oxidizing bacteria ( NOB ) ratio and the average DO concentrations in the last aeration periods ; when the average DO concentrations in the last aeration periods were lower than 4.86 mg / L , AOB became the dominant nitrifier population , which benefited
nitrogen removal via
PND . -
pubmed
To accomplish efficient
nitrogen removal via
PND , the optimum aeration rate ( A , L air / min ) applied can be predicted according to the average organic loading rates based on mathematical equations developed in this study . -
pubmed
He had a heterozygous germline
CARD11 mutation causing a G123D
amino acid substitution , which was demonstrated to induce
NF - kappaB activation in unstimulated lymphocytes . -
pubmed
And the results of Western blot showed that
hesperetin caused an increase in the levels of cytosolic
AIF , cytosolic
Apaf-1 , cytosolic
Cyt C ,
caspase-3 ,
caspase-9 and
Bax and a decrease in that of
Bcl-2 , mitochondrial
AIF , mitochondrial
Apaf-1 and mitochondrial
Cyt C ( P & lt ; 0.05 ) . -
pubmed
Nucleotide - binding oligomerization domain-2 (
NOD2 ) is an innate immune receptor that recognizes peptidoglycan - derived
muramyl dipeptide from intracellular bacteria and triggers proinflammatory signals . -
pubmed
Owing to their intrinsic estrogenic activity , at maximal concentrations ,
OH - TORE and
OH - OSPE blocked the stimulatory effect of
E2 by only 89 % compared to 100 % with
ACOL . -
pubmed
Concentration - response curves to
phenylephrine (
PHE ) were obtained from aortic rings incubated with
N ( G )- nitro - l - arginine methyl ester (
l - NAME ) ,
superoxide dismutase (
SOD ) ,
indomethacin ,
SC-560 , and
NS-398 . -
pubmed
Glycine augmented the sensitivity for
PHE in the presence of
l - NAME and
SOD . -
pubmed
Glycine increased the mRNA expression of
eNOS and decreased the expression of
COX-2 and
TNF - alpha . -
pubmed
Cell proliferation , mineralized
calcium deposition ,
alkaline phosphatase (
ALP ) activity and apoptosis were assessed at various time - points . -
pubmed
The 58 subjects were assessed during each phase of the study for clinical presentation of
gingivitis and concurrently had plaque sampled for real - time
polymerase chain reaction ( RTPCR ) microbiological characterization and salivary lavage samples for ' systems biology ' metabonomics assessment by
1H - NMR . -
pubmed
OBJECTIVE : The purpose of this study was to investigate the pathogenic role of
RyR - bound
CaM in diastolic
Ca2 + leakage from the sarcoplasmic reticulum and arrhythmogenesis in pressure - overloaded
heart failure . -
pubmed
The frequency of spontaneous
Ca2 + transients in TAC cells during and after 1 - 5 Hz of field stimulation was 44 % , whereas it was significantly attenuated by HA - CaM but not with
CaM. -
pubmed
CONCLUSION : Several disorders in the
RyR channel function characteristic of pressure - overloaded cells ( increased spontaneous
Ca2 + leakage , delayed afterdepolarization , triggered activity ,
Ca2 + spark frequency , spontaneous
Ca2 + transients ) are caused by deteriorated
CaM binding to
RyR2 . -
pubmed
Twenty - eight SNPs associated with TGs , 39 SNPs associated with total
cholesterol (
TC ) , 28 SNPs associated with
LDL cholesterol (
LDL - C ) , and 56 SNPs associated with
HDL cholesterol (
HDL - C ) were analyzed individually and combined into genetic risk scores ( GRSs ) . -
pubmed
Cholesterol - dependent QS-21 endocytosis followed by lysosomal destabilization and
Syk kinase activation were prerequisites for this response . -
pubmed
OBJECTIVE : To assess whether type 2
inflammation , as measured by sputum eosinophils and fractional exhaled
nitric oxide ( FeNO ) , is a feature of naturally occurring virus - induced exacerbations of
asthma and whether
TSLP is associated with this type 2
inflammation . -
pubmed
In the framework of the VATO project , the model has been tested against two - year - long in situ measurements of
3H activity concentration monitored by IRSN in air , groundwater and grass , together with meteorological parameters , on a grass field plot located 2 km downwind of the AREVA
NC La Hague nuclear reprocessing plant , as was done in the past for the evaluation of transfer of
14C in grass . -
pubmed
Further experimental investigation will be undertaken by IRSN and
EDF next year to better evaluate ( and properly model ) other aspects of
tritium transfer where knowledge gaps have been identified in both experimental and modelling areas . -
pubmed
The Waters BEH
C18 column was used with a gradient mobile phase system of
acetonitrile and water containing 0.1 %
formic acid . -
pubmed
NLRP4 is an essential negative regulator of
fructose - induced
cardiac injury in vitro and in vivo . -
pubmed
Here , we illustrated that
NLRP4 attenuates pro - inflammatory
cytokines releasing , including
Transforming growth factor ( TGF - beta1 ) ,
Tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1beta (
IL-1beta ) ,
interleukin-18 (
IL-18 ) and
interleukin-6 (
IL-6 ) , in
fructose - treated cardiac cells by means of RT - qPCR , and western blotting analysis . -
pubmed
In vivo , high
fructose feeding induced
cardiac injury , accompanied with reduced expression of
NLRP4 in heart tissue samples , indicating the possible role of
NLRP4 in ameliorating
heart injury . -
pubmed
Dopamine D2 Receptors in the Paraventricular Thalamus Attenuate
Cocaine Locomotor Sensitization . -
pubmed
Alterations in thalamic
dopamine ( DA ) or
DA D2 receptors (
D2Rs ) have been measured in
drug addiction and
schizophrenia , but the relevance of thalamic
D2Rs for behavior is largely unknown . -
pubmed
We therefore hypothesise that
bleomycin injections in combination with electroporation-'electrosclerotherapy ' (
EST ) , also known as ' electrochemotherapy'-could potentially be a novel alternative treatment option for
CMs . -
pubmed
Three regions of interest ( ROIs ) within the CM will be randomly allocated for treatment with ( A )
EST , ( B )
bleomycin sclerotherapy without electroporation and ( C ) no treatment . -
pubmed
However , the proportion of
somata containing
M1R among cortical cellular types , and the precise intracellular localization of
M1R remain unclear . -
pubmed
M1R immunopositivity signals were found in the majority of the
somata of both pyramidal neurons and inhibitory neurons . -
pubmed
In pyramidal neurons , strong
M1R immunopositivity signals were usually found throughout their
somata and dendrites including spines . -
pubmed
On the other hand , the signal strength of
M1R immunopositivity in the
somata of inhibitory neurons significantly varied . -
pubmed
In PV neurons ,
M1R immunopositivity signals were preferentially distributed in
somata . -
pubmed
RESULTS : There is accumulating evidence of increased sensitivity to
platinum - based therapy and
poly - ADP - ribose polymerase inhibitors ( PARPi ) in
BRCA - associated
PDAC . -
pubmed
Patients with
BRCA - associated locally advanced
PDAC can benefit from targeted therapy with PARPi (
olaparib ) as a second - line therapy after antimetabolite treatment failure . -
pubmed
Patients with unresectable metastatic
BRCA - positive
PDAC may benefit from
platinum - based therapy . -
pubmed
The aim of the present study was to investigate whether
Dicer , a key miRNA biogenesis enzyme , and miRNAs in macrophages are implicated in the anti - inflammatory effects of
metformin . -
pubmed
MATERIALS AND METHODS : Enzyme - linked immunosorbent assay and reverse transcription quantitative
polymerase chain reaction were carried out to verify the anti - inflammatory effects of
metformin . -
pubmed
Western - blotting , enzyme - linked immunosorbent assay and reverse transcription quantitative
polymerase chain reaction were used to examine the role
Dicer and miRNAs play in the anti - inflammatory effects of
metformin . -
pubmed
RESULTS : In parallel with the suppression of
interleukin-6 and
tumor necrosis factor - alpha production in resting and lipopolysaccharide - stimulated macrophages ,
metformin could induce an increase in
Dicer and most miRNAs . -
pubmed
When
Dicer was knocked down , the anti - inflammatory effects of
metformin were significantly attenuated . -
pubmed
Additionally , the upregulation of miRNA ( miR)-34a-5p and miR-125b-5p by
metformin were also blunted in
Dicer knockdown macrophages . -
pubmed
CONCLUSIONS :
Metformin might show anti - inflammatory effects in macrophages through the induction of
Dicer and the subsequent upregulation of miR-34a-5p and miR-125b-5p . -
pubmed
gamma - Thialysine versus
Lysine : An Insight into the Epigenetic Methylation of
Histones . -
pubmed
Biomedicinally important
histone lysine methyltransferases ( KMTs ) transfer a
methyl group from
S - adenosylmethionine to
lysine residues in
histones and other proteins . -
pubmed
Here , we report comparative studies on epigenetic methylation of
lysine and
gamma - thialysine , the simplest
cysteine - derived
lysine analog , which can be introduced to histone peptides and
histone proteins via site - specific bioconjugation - based
cysteine alkylation . -
pubmed
This work provides a molecular basis for the application of
gamma - thialysine for biomolecular studies of intact
histones and the nucleosome assembly . -
pubmed
Examples using
pipecolic acid for the construction of
piperidine derivatives , as well as the one -
pot synthesis of
alpha - amino nitriles , are also shown . -
pubmed
The
Aurora kinases represent a group of
serine /
threonine kinases which are crucial regulators of mitosis . -
pubmed
We report that
AurkB undergoes acetylation in vitro by
lysine acetyltransferases (
KATs ) belonging to different families , namely by
p300 and
Tip60 . -
pubmed
The haploinsufficient
tumor suppressor
Tip60 acetylates two highly conserved
lysine residues within the kinase domain of
AurkB which not only impinges the protein stability but also its kinase activity . -
pubmed
Anabolic
steroids (
AS ) are synthetic
testosterone - derivatives developed by the pharmaceutical industry to mimic
testosterone biological effects . -
pubmed
In conclusion , patients with
lung adenocarcinoma harboring the
EGFR S768I and G724S mutations appear less sensitive to
icotinib than patients with sensitive
EGFR . -
pubmed
If
APRT deficiency is recognized early ,
renal failure can be prevented , arrested or reversed in native kidney and in allograft by treatment with
allopurinol , which inhibits
xanthine oxidase and reduces
2,8 - DHA formation . -
pubmed
Tunable absorber embedded with
GST mediums and trilayer
graphene strip microheaters . -
pubmed
The absorber configuration exploits the
Ge2Sb2Te5 (
GST ) phase changing medium , and the effects of different parametric and operational conditions on the absorption spectra were explored . -
pubmed
A controlled clinical trial of
streptokinase and
heparin in the treatment of major
pulmonary embolism . -
pubmed
Streptokinase of
heparin was given for 72 hours and pulmonary angiography was repeated . -
pubmed
Acute
respiratory and metabolic alkalosis significantly reduced serum
potassium by about 26 % , while chronic
furosemide treatment ( 1 mg / kg
IV daily for 14 + /- 4 days ) significantly reduced both serum
potassium concentration ( 4.16 + /- 0.31 to 3.27 + /- 0.14 mEq / L ) and skeletal muscle
potassium content ( 80.9 + /- 5.6 to 58.7 + /- 4.1 mEq / kg ) . -
pubmed
After rapid in vivo hydrolysis of the
nafate pharmaceutical form to
cefamandole (
II ) , the antibiotic was found to be very resistant to metabolic degradation in both species . -
pubmed
LCAT activity is determined measuring the
cholesterol esterification , and is expressed as micrograms of
cholesterol esters formed per milliliter plasma per hour . -
pubmed
A 2nd type of dietary constituent affecting the
microsomal mixed - function oxidase system is added
phenolic antioxidant , i.e. -
pubmed
The
cytochrome P-450 shows altered spectral characteristics , and the
aryl hydrocarbon hydroxylase system of these microsomes has an increased sensitivity to inhibition by
alpha - naphthoflavone . -
pubmed
In addition , there is a decrease in binding of metabolites of
benzo ( a ) pyrene to DNA upon incubation of these microsomes of induction of increased mixed function
oxidase activity have shown that increased levels of activity protect against administration of chemical carcinogens . -
pubmed
The thoracic accumulation of neutrophils labelled with
111Indium - oxine in response to infusion of
platelet activating factor (
PAF , 18 ng / kg / min x 5 min , i.v . ) -
pubmed
Pacemaker and spindle - like oscillations were unaffected by
tetrodotoxin (
TTX ) or by selective blockade of
NMDA , non - NMDA , GABAA , GABAB , nicotinic , muscarinic , alpha - and beta - noradrenergic receptors . -
pubmed
Inspection of the
amino acid sequence deduced from
nucleotide sequence of
lukS and that from S - component of
leukocidin clarified that pre - matured S - component contains a typical signal sequence at the NH2 terminus . -
pubmed
The 58 - kDa subunit of the microsomal transfer protein complex was identified as
protein disulfide - isomerase (
PDI ) (
EC 5.3.4.1 ) by 1 ) a comparison of the
amino - terminal sequence of
PDI and the 58 - kDa subunit of the transfer protein , 2 ) a comparison of the reverse phase high performance liquid chromatography peptide maps of
CNBr digests of
PDI and the
lipid transfer protein , 3 ) immunoprecipitation competition experiments in which
PDI was found to compete with the
lipid transfer protein for immunoprecipitation by the anti-58 - kDa polyclonal antibodies , 4 ) immunological cross - reactivity of the
microsomal triglyceride transfer protein complex with polyclonal antibodies raised against
PDI , and 5 ) the appearance of
protein disulfide isomerase activity following the dissociation of purified
microsomal transfer protein complex with
guanidine HCl . -
pubmed
Determination of serum
rifampicin (
RFP ) after dose of the drug is often requested not only for the clinical criteria on its use , but also for the check of its adverse reactions . -
pubmed
In comparative examination on each clinical use of 2 kinds of
RFP commercial products :
Rifadin and
Aptesin , the authors had a chance of simultaneous determination of serum
RFP by means of 3 different assay methods : solvent extraction method (
SE ) , biological activity method ( BA ) and liquid - chromatography method ( LC ) . -
pubmed
The highest determinations were presented by the
SE , which was devised as total assay method determining
desacetyl RFP and
3 - formyl rifamycin SV besides free
RFP . -
pubmed
Thus the difference between both determinations by
SE and LC was caused by
RFP metabolites . -
pubmed
Some attensions were called to the practical estimation of the serum
RFP concentrations : Clear individual differences were found in the serum
RFP concentrations after dose of the
drug , and appeared to become clearer in the stage of its continuous administrations . -
pubmed
It was shown that the difference in UDS rates between control and
ACR fibroblast
strains was not due to
[ 3H ]- dThd catabolism and/or disappearance in the culture medium after a 34 - h labelling period . -
pubmed
When the percent increases in
N - acetoxy-2 - acetylaminofluorene (
NA - AAF)-induced UDS and in
[ 3H ]- dThd incorporation in the presence of 10 mM
hydroxyurea were compared between 24 h and 34 h incubation , the 13
ACR fibroblast lines were significantly lower compared to the corresponding values calculated for the seven control fibroblast
strains . -
pubmed
Binding of
DT to asolectin or
DL - alpha - dipalmitoylphosphatidylcholine - DL - alpha - dipalmitoylphosphatidic acid liposomes at pH 7.3 results in a 10 % increase in its alpha - helix content . -
pubmed
At pH 4 , in the presence of liposomes , the secondary structure of
DT is characterized by the appearance of a beta - sheet structure with strengthened
hydrogen bonds which did not exist before pH lowering . -
pubmed
However , the alpha - helix content of
CB1 increases by 10 % , and polarization measurements indicate that the alpha - helices of
CB1 at pH 4 are oriented parallel to the lipid
acyl chains . -
pubmed
The obtained data suggest the possibility of action of
kynurenine and
quinolinic acid via
picrotoxin - sensitive subunits of the
benzodiazepine - GABA - receptor - ionophore complex . -
pubmed
The isometric contraction to
phenylephrine was antagonised competitively by
prazosin with a pA2 value of 9.45 , suggesting that the receptor is an
alpha 1 - adrenoceptor . -
pubmed
The drug succeeded in normalizing the
cholesterol and
triglyceride serum levels in acute as well as in chronic
hyperlipaemia , and , in this connection , it provoked a significant increase of serum
alpha - lipoproteins and a decrease of serum
beta - lipoproteins , with a consequent improvement of the beta / alpha ratio . -
pubmed
Analysis of plasma lipoproteins indicated that
sucrose elevated
VLDL and
LDL when compared to
lactose . -
pubmed
The increase in
lysozyme secretion by these cells , however , is markedly blunted suggesting that continued
PMA treatment of differentiated cells is required for their secretion of
lysozyme . -
pubmed
There is a modest ( five - fold ) increase in lysozyme mRNA between 0 and 36 h of exposure to
PMA , corresponding to the burst of
lysozyme secretion by these cells . -
pubmed
Wild - type cells labeled in their DNA with
[ ( 3 ) H ] thymidine in the presence of
TP produced small pieces of DNA ( 7 x 10(6 ) daltons ) , which corresponded in size to late replicative intermediates of discontinuous DNA synthesis . -
pubmed
[
Phospholipid metabolism in
pancreatitis -- effects of
CDP -
choline on
phospholipase A ] . -
pubmed
Osteoporosis , skin
collagen , and
androgen . -
pubmed
Decreased body
collagen may possibly be a primary defect in
osteoporosis , while
androgens may increase total skin
collagen . -
pubmed
Inhibition of
histamine - induced
nasal symptoms by the
H1 antihistamine
chlorpheniramine maleate : demonstration of topical effect . -
pubmed
Progesterone or
HPL treatments alone did not substantially influence LI . -
pubmed
HPL treatment , but not
progesterone or
thyroxine treatments significantly enhanced the stimulatory effect of
estrogen on LI . -
pubmed
The mean plasma concentration - time curve ( Cp ) after intravenous
ampicillin sodium administration was described biexponentially , as : Cp = 38.8 e -0.0268 t + 0.45 e -0.0058 t. Intramuscular injection , into the lateral neck , of Ampikel-20 and Polyflex resulted in 100 per cent bioavailabilities within 12 h
post injection ( p.i . ) -
pubmed
The specific activity of
pig muscle D - glyceraldehyde-3 - phosphate dehydrogenase was found to be constant in the reverse reaction , with
NADH and
1,3 - diphosphoglycerate as substrates , over the
enzyme concentration range 10(-8 ) to 10(-4 ) M. The molecular weight of the covalent intermediate of the
enzyme ,
3 - phosphoglyceroyl - glyceraldehyde-3 - phosphate dehydrogenase , as measured by sedimentation techniques , proved constant ( 145 000 + /- 6 000 ) between 3 x 10(-5 ) M
enzyme concentration . -
pubmed
A Scatchard analysis showed that the
rRNA - binding immunoglobulin is monoclonal and has a high affinity ( 10(9 ) liter / mole ) for the RNA
antigen . -
pubmed
It has been successful to locate the antigenic determinant of one of these
hemoglobins within a defined
amino acid sequence . -
pubmed
Pretreatment of rings with
indomethacin or
adenosine or
endothelin receptor antagonists had no effect on the
hypoxia - mediated alterations . -
pubmed
Monoclonal antibody 15A9 was shown to counteract
GTP - induced inhibition , while 1F2 influenced the conformation of
E1 , allowing two antibodies , which did not previously bind
E1 , to bind to it . -
pubmed
The vaccine consisted of the non - glycosylated FeLV
envelope protein p45 ,
aluminium hydroxide and a
saponin adjuvant . -
pubmed
IGF - I treatment led to reduced fasting and stimulated (
glucose and/or
L - arginine )
insulin and growth hormone secretion . -
pubmed
Intravenous
glucose tolerance was unaltered despite reduced
insulin secretion . -
pubmed
Resting energy expenditure and lipid oxidation were both elevated , while protein oxidation was reduced , and
glucose turnover rates were unaltered on the fifth treatment day with
IGF - I as compared to the control period . -
pubmed
Moreover ,
insulin - stimulated oxidative and nonoxidative
glucose disposal ( i.e. -
pubmed
Thus ,
IGF - I treatment leads to marked changes in lipid and protein oxidation , whereas , at the dose used ,
carbohydrate metabolism remains unaltered in the
face of reduced
insulin levels and enhanced
insulin sensitivity . -
pubmed
TEM - EDS analyses indicated that
Ca and P were the major elements , with small amounts of
Mg . -
pubmed
DHT administration in prepuberal castrates stimulated
collagen synthesis and accumulation in the stroma of male accessory sex organs as well as the proliferation and differentiation of the glandular epithelium in these organs . -
pubmed
Immune complex - stimulated neutrophil
LTB4 production is dependent on
beta 2 integrins . -
pubmed
However , both genetically
beta 2 - deficient PMN and PMN treated with the
anti - beta 2 mAb IB4 subsequently detached from the IC - coated surfaces . -
pubmed
Importantly ,
LTB4 production by PMN stimulated with fluid phase heat - aggregated
IgG also required the
beta 2 integrins , showing that the defect was not a simple consequence of abnormal adhesion . -
pubmed
The initial rises in intracytoplasmic [
Ca2 +]i in response to aggregated
IgG also were unaffected by inhibition of
beta 2 integrins . -
pubmed
Finally ,
LTB4 production by normal PMN adherent to ICs was inhibited by antibodies to
FcRII , but not
FcRIII , showing that
FcRII ligation was required for this effect . -
pubmed
Together these data identify a role for the
beta 2 integrins in a signal transduction pathway leading to sustained adhesion and
LTB4 production in response to IC . -
pubmed
Anti - Thy 1.1 and anti -
desmin antibodies recognizing glomerular mesangial cells and antibodies against
O - acetyl GD3 and
gp330 antigens of podocytes , as well as anti -
thrombospondin and anti -
cytokeratin antibodies identifying parietal epithelial cells failed to bind to our cells . -
pubmed
Involvement of the
CoA - independent transacylase demonstrated by analysis of the molecular species of
platelet - activating factor . -
pubmed
Consistent with the involvement of
arachidonate - specific CoA - independent transacylase in the synthesis of
platelet - activating factor (
PAF ;
1 - O - alkyl-2 - acetyl - GroPCho ) , 16:0 and 18:1
PAF species were formed upon
antigen stimulation [ Joly , F. , Breton , M. , Wolf , C. , Ninio , E. & amp ; Colard , O. -
pubmed
Consistent with
alkyl - arachidonyl - GroPCho species content and
acetyltransferase specificity , similar amounts of 16:0 and 18:1
PAF species were formed when mast cells were stimulated with
antigen . -
pubmed
More interestingly , addition of
16:0 lyso - PAF almost doubled the amount of 18:1
PAF content as compared to
antigen alone , thus indicating that the
lyso - PAF formed via the
CoA - independent
transacylase was significantly used for
PAF synthesis , despite a large excess of exogenous
lyso - PAF . -
pubmed
However ,
PAF is preferentially synthesized through a mechanism involving the
CoA - independent
transacylase reaction . -
pubmed
The decreased
androgen /
estrogen ratio , and the increased
estrogen value could be responsible for an
estrogen receptor mediated effect on the tubular epithelial cell proliferation , an event further potentiated by the action of regulatory peptides like
epidermal growth factor (
EGF ) . -
pubmed
Interactions of
alpha - chymotrypsinogen A with
alkylureas . -
pubmed
Solvation of
alpha - chymotrypsinogen A (
alpha - ctg A ) in aqueous
urea ,
methylurea ,
N , N '- dimethylurea and
ethylurea was studied by density measurements . -
pubmed
UNLABELLED : Renal proximal tubular cells have been shown to express
aromatic L - amino acid decarboxylase (
L - AAAD ) , which converts
L - dopa into
dopamine and
5 - hydroxytryptophan [
( OH ) Trp ] into
5 - hydroxytryptamine (
5 - HT ;
serotonin ) . -
pubmed
Because
5 - HT receptors have been demonstrated in proximal cells , we hypothesized that
5 - HT may act as an autocrine / paracrine modulator of proximal transport . -
pubmed
5 - HT reversed
parathyroid hormone (
PTH)-induced
cAMP accumulation in a
pertussis toxin - sensitive manner and reduced the
PTH inhibition of P(i ) uptake without affecting the
NaP ( i )- 4 mRNA level . -
pubmed
The effects of
5 - HT on
cAMP generation and
Na - P ( i ) co - transport were reproduced by
( OH ) Trp , except in the presence of
benserazide , and by
L - propranolol and
dihydroergotamine , two
5 - HT1B receptor agonists . -
pubmed
Linear
IgA bullous dermatosis after contact with
sodium hypochlorite . -
pubmed
A bifunctional enzyme ,
fructose-6 - phosphate 2 - kinase - fructose 2 , 6 - bisphosphatase , catalyzes synthesis and degradation of
fructose 2 , 6 - bisphosphate . -
pubmed
Mutants of basic residues , including
Lys51 ,
Arg78 ,
Arg79 ,
Arg136 ,
Lys172 , and
Arg193 , immediately around the active site of
rat testis fructose 6 - P,2 - kinase were constructed , and their steady state kinetics ,
ATP binding , and the effect of pH on the kinetics were characterized . -
pubmed
Substitution of
Lys51 , which is located in a Walker - A motif ( GXXGXGKT ,
amino acids 45 - 52 ) , with
Ala or
His resulted in
enzymes with increased KMgATP values and unable to bind
Fru 6 - P. -
pubmed
The pH dependence of V and the V / Ks for
MgATP and
Fru 6 - P suggest a mechanism in which reactants and
enzyme combine irrespective of the protonation state of groups required for binding and catalysis , but only the correctly protonated
enzyme - substrate complex is catalytically active . -
pubmed
The application of negative upper airway pressure ( 0 to -12.5 cm
H2O ) caused increases in levator palatini (
LP , p & lt ; 0.001 ) and palatoglossus ( PG , p & lt ; 0.001 ) activity , 100 msec after pressure stimulus in normal subjects . -
pubmed
Permeability of
beta - lactams across the outer membrane (
OM ) of M2 was lower than that across the
OM of C152 . -
pubmed
Mutants deficient in the 43 - kDa
OMP could be selected in vitro from strain C152 in the presence of
cefoxitin . -
pubmed
During
carbon starvation ,
DnaK deficiency reduced
RpoS levels threefold , while
DnaK excess increased
RpoS levels nearly twofold . -
pubmed
Important parameters involved in the activation of host - reactive T cells by nonspecific infectious agents included the presence , localization , and quantity of the specific transgene - encoded self -
antigen ; in contrast ,
CD4 + T cells were not required . -
pubmed
Following metabolic labeling of these cells with
[ 14C ] galactose , the
GSL were purified and the radioactivity incorporated into each individual
GSL quantitated by phosphoimaging . -
pubmed
Dysregulation of
aquaporin-2 (
AQP2 ) , the predominant
vasopressin - regulated
water channel , is known to be associated with a range of
congenital and acquired water balance disorders including
nephrogenic diabetes insipidus and states of water retention . -
pubmed
To measure the breakdown of
TMH - ferrocene ,
iron release from the molecule was analyzed using a Ferrochem
II analyzer , which uses constant potential coulometry to measure the amount of ionic
iron within a biological sample . -
pubmed
The
TMH - ferrocenase activity is heat labile , requires a physiologic temperature , is induced by
phenobarbital , and is inhibited by
carbon monoxide and
piperonyl butoxide but not by
dicoumarol . -
pubmed
The
enzyme follows Michaelis - Menten kinetics and has a Km of 58.5 microM and a Vmax of 57.5 nmol
Fe released / mg protein / min . -
pubmed
We conclude that
TMH - ferrocene is degraded by a
phenobarbital - inducible
cytochrome P-450 . -
pubmed
They have been found to interfere at different stages of T cell activation and proliferation , and can be identified as inhibitors of
nucleotide synthesis ,
growth factor signal transduction and differentiation . -
pubmed
Cocaine - seeking behavior and
Fos expression in the amygdala produced by
cocaine or a
cocaine self - administration environment . -
pubmed
Optimal
AChE mRNA localization was obtained with a
digoxigenine - labeled RNA probe . -
pubmed
Carbonic anhydrase inhibitors :
N - cyanosulfonamides , a new class of high affinity
isozyme II and IV inhibitors . -
pubmed
Compounds of the types RSNHCN and
RCONHCN were much less active in inhibiting all the investigated
isozymes as compared to the strong inhibitors possessing the general formula
RSO2NHCN . -
pubmed
Susceptibility to inhibition with the
N - cyanosulfonamides was generally : hCA
II & gt ; bCA
IV & gt;> ;
hCA I. -
pubmed
Spectroscopic studies on
Co ( II )-substituted hCA
II proved that the new inhibitors directly bind to the metal ion within the enzyme active site , similarly to the classical inhibitors of the unsubstituted
sulfonamide type . -
pubmed
Because many agonists stimulate ciliary motility through activation of
cyclic nucleotide - dependent protein kinases , we hypothesized that
ethanol stimulates ciliary motility by activating
protein kinases . -
pubmed
RESULTS :
Ethanol markedly activated
cyclic adenosine monophosphate ( cAMP )- dependent protein kinase [
protein kinase A (
PKA ) ] at biologically relevant concentrations ( 20 - 100 mM ) , with activation detectable after 15 min and persisting up to 4 hr . -
pubmed
Ethanol 's
PKA activation was blocked by
nitric oxide synthase inhibitors , indicating a
nitric oxide - dependent pathway , and was also specifically blocked by
PKA inhibitors . -
pubmed
Selective recognition of
mannose by the
human eosinophil Charcot - Leyden crystal protein (
galectin-10 ): a crystallographic study at 1.8 A resolution . -
pubmed
In the absence of any known natural
carbohydrate ligand for this protein , the functional role of the
CLC protein (
galectin-10 ) has remained speculative . -
pubmed
Here we describe structural studies on the
carbohydrate binding properties of the
CLC protein and report the first structure of a
carbohydrate in complex with the protein . -
pubmed
Interestingly , the
CLC protein demonstrates no affinity for
beta - galactosides and binds
mannose in a manner very different from those of other related
galectins that have been shown to bind
lactosamine . -
pubmed
The partial conservation of residues involved in
carbohydrate binding led to significant changes in the topology and chemical nature of the
CRD , and has implications for
carbohydrate recognition by the
CLC protein in vivo and its functional role in the biology of
inflammation . -
pubmed
Insulin - mediated vasodilation and
glucose uptake are independently related to fasting serum nonesterified
fatty acids in elderly men . -
pubmed
Insulin - mediated
glucose disposal was related to
body mass index ( BMI ) ( r = -0.49 , P = 0 . -
pubmed
In multivariate analysis only
BMI and
NEFA remained significantly related to whole - body
glucose uptake . -
pubmed
CONCLUSIONS : The study demonstrates that in elderly men
BMI and fasting serum
NEFA but not
insulin - induced vasodilation are related to
insulin sensitivity . -
pubmed
Monoclonal antibody
MIB-5 or antibodies to
bromodeoxyuridine were used to identify proliferating cells . -
pubmed
Determination of the
disulfide bond and its possible role in
tobacco acetolactate synthase . -
pubmed
The mechanisms that influence the polarization of
CD4 T cells specific for allogeneic
MHC class II molecules in vivo are still poorly understood . -
pubmed
SDS - PAGE analysis of isotope - labelled
HSV - specific proteins revealed that the synthesis of
beta proteins was moderately inhibited and some of the
glycoproteins were synthesized with reduced molecular weights . -
pubmed
From these findings , antiherpetic activities of
Conmy A and B might be mainly dependent on their activities as
vacuolar proton - ATPase inhibitors with intracellular translocation of
glycoproteins and the inhibition of the maturation of virus
glycoproteins . -
pubmed
We used
leptomycin B (
LMB ) , an inhibitor of nuclear export signal ( NES)-and its receptor ,
CRM1 /
Exportin1 - mediated protein export . -
pubmed
LMB efficiently inhibited
vRNP export , while it did not affect the subcellular localization and export of
matrix protein ( M ) 1 and
nonstructural protein ( NS ) 2 . -
pubmed
Second , indirect immunofluorescence assays also revealed that
vRNP export is sensitive to
LMB . -
pubmed
Analyses using
glycerol density gradients showed that a major fraction of
M1 and
NS2 was not complexed with the exported
vRNP . -
pubmed
LMB - sensitive and -insensitive
NS2 . -
pubmed
BACKGROUND : The crystal structure of
precorrin-8x methyl mutase (
CobH ) , an
enzyme of the aerobic pathway to
vitamin B12 , provides evidence that the mechanism for
methyl migration can plausibly be regarded as an allowed [ 1,5]-sigmatropic shift of a
methyl group from C-11 to C-12 at the C ring of
precorrin-8x to afford
hydrogenobyrinic acid . -
pubmed
The same level of DeltaPes was observed at the end of OHs and RERAs ( 21.9 + /- 5.5 versus 18.9 + /- 5.7 cm
H ( 2 ) O respectively [
NS ] ) whereas the reduction in flow was as expected , higher for
OH ( 57.9 + /- 10.7 versus 21.3 + /- 4.9 % ) . -
pubmed
The mitogen- and stress - induced phosphorylation of
CREB at
Ser133 has been linked to the transcription of several immediate early genes , including c - fos ,
junB , and
egr1 . -
pubmed
The knockout of both
MSK1 and
MSK2 resulted in a 50 % reduction in c - fos and
junB gene transcription in response to
anisomycin or UV - C radiation but only a small reduction in response to
tetradecanoyl phorbol acetate or
epidermal growth factor in fibroblasts . -
pubmed
Chromatographic results suggest that
BNP interacts approximately the same with both the C- and
N - terminal amino acid of
poly SULL , while the preferential site of interaction of this analyte with the monomeric form of
SULL ( mono SULL ) is at the C - terminal
amino acid . -
pubmed
This effect was antagonized by
WIN55,212 - 2 and abolished by
N - ethylmaleimide , consistent with the involvement of
cannabinoid CB ( 1 ) receptors through the activation of
G ( i / o ) proteins . -
pubmed
Following sequences were acquired : T 2 -w TSE and T 1 -w FLASH , after administration of
gadolinium , 6 VIBE sequences with iPAT (
TR / TE / matrix / partition thickness / time of acquisition : 6.2 ms/ 3.2 ms/256 x 192/4 mm/13 s ) , as well as T 1 -weighted FLASH with fat saturation . -
pubmed
Myostatin is a potent
inhibitor of muscle growth . -
pubmed
METHODS : 15
EAs were observed by means of
HE staining , immunohistochemical staining ( ABC method ) and in part by
electron microscopy . -
pubmed
on the pharmacokinetics and brain distribution of the
adenosine A1 receptor partial agonist
2 '- deoxy - N6 - cyclopentyladenosine (
2'dCPA ) , and ( 2 ) the effect of
2'dCPA ( 20 mg / kg i.v . ) -
pubmed
VEGF concentrations in the cell culture supernatant were determined by enzyme - linked immunosorbent assay ( ELISA ) , and cell proliferation was determined by
MTT assay and colony - forming assay in vitro . -
pubmed
This is supplemented with HBO given in 90 - minute sessions at approximately 2.5
ATM of pure
oxygen for 20 sessions prior to surgical intervention and followed by 10 additional postoperative dives . -
pubmed
A prior comparison of 750 mg
flutamide daily to 500 mg daily with an
LHRH analog or orchiectomy showed no difference in effect on
prostate specific antigen (
PSA ) . -
pubmed
Serum samples at the initiation of therapy and at the 1- and 3 - month time point were assessed for
PSA ,
testosterone , liver function tests , hematology , and renal function . -
pubmed
Testosterone and
PSA levels show a dose - dependent response to
flutamide monotherapy . -
pubmed
Flutamide at 500 mg did not result in significant changes in
testosterone ,
PSA , prostate volume , or
androgen deficiency symptoms compared to 750 mg daily after 3 months . -
pubmed
Deletion and aberrant
CpG island methylation of
Caspase 8 gene in
medulloblastoma . -
pubmed
We determined the frequency of aberrant
CpG island methylation for
Caspase 8 (
CASP8 ) in a group of 24
medulloblastomas arising in 8 adult and 16 pediatric patients . -
pubmed
Mucosa - free circular muscle strips were precontracted with
methacholine in the presence of
guanethidine and exposed to electrical field stimulation (
EFS ) and exogenous
NO ,
ATP , VIP and
PACAP . -
pubmed
NO ( 10(-6)-10(-4 ) m)-induced concentration - dependent relaxations that were inhibited by the soluble
guanylyl cyclase inhibitor
1H -[ 1,2,4 ]- oxadiazolo -[ 4,3 - a ]- quinoxalin-1 - one (
ODQ ; 10(-5 ) m ) and the
small conductance Ca ( 2 +)- activated K (+)- channel blocker
apamin (
APA ; 3 x 10(-8 ) m ) . -
pubmed
Relaxations elicited by exogenous
ATP ( 10(-4)-10(-3 ) m ) were inhibited by the
P2Y purinoceptor antagonist reactive blue 2 (
RB2 ; 3 x 10(-4 ) m ) , but not by
APA and
ODQ . -
pubmed
The inhibitory responses evoked by 10(-7 ) m
VIP and 3 x 10(-8 ) m
PACAP were decreased by the selective
PAC ( 1 ) receptor antagonist
PACAP(6 - 38 ) ( 3 x 10(-6 ) m ) and
APA . -
pubmed
The
VPAC ( 2 ) receptor antagonist
PG99 - 465 ( 3 x 10(-7 ) m ) reduced relaxations caused by
VIP , but not those by
PACAP , while the
VPAC ( 1 ) receptor antagonist
PG97 - 269 ( 3 x 10(-7 ) m ) had no influence . -
pubmed
EFS - induced relaxations were inhibited by the
NO - synthase inhibitor
N ( omega )- nitro - l - arginine methyl ester ( 3 x 10(-4 ) m ) ,
ODQ and
APA , but not by
RB2 ,
PG97 - 269 ,
PG99 - 465 and
PACAP ( 6 - 38 ) . -
pubmed
Here , we compared two methods of
GAD65Ab epitope analysis :
GAD65 / 67 fusion proteins and competition assays using
GAD65 - specific recombinant fraction
antigen binding (
rFab ) . -
pubmed
Fusion proteins allow the definition of large epitope regions ; however , some conformational
GAD65Ab epitopes , especially those residing in the middle region , are destroyed or distorted in the fusion proteins . -
pubmed
This treatment did not increase the functional affinity for
acetylcholine at
type II nAChRs . -
pubmed
A single injection of
nicotine also produced a significant increase in
type III nAChR response . -
pubmed
In addition , type III and type
II , but not
type IA nAChRs , are desensitized by in vitro exposure to
nicotine at concentrations found in the venous blood of cigarette smokers . -
pubmed
Bupropion at 1 muM produced 56 , 15 , and 0 % inhibition of
type III , type II , and type IA nAChR responses , respectively , in the slices . -
pubmed
Our results suggest that in vivo -
nicotine - induced
nAChR up - regulation observed in neurons of intact brain tissue is a physiologically relevant phenomenon and that early up - regulation of
type III and type II nAChRs could be an important biological signal in
nicotine addiction . -
pubmed
Abeta and its precursor protein (
APP ) interact with metal ions such as
zinc ,
copper and
iron . -
pubmed
Insulin - mediated signal transduction is negatively regulated by the
phosphotyrosine phosphatase ,
PTP1B , and numerous studies have demonstrated that
organo - vanadium compounds , which are nonselective
phosphotyrosine phosphatase inhibitors , have
insulin - mimetic properties . -
pubmed
However , whether or not
vanadium compounds can prevent the transition from
insulin resistance to overt
diabetes is unknown . -
pubmed
Roxithromycin inhibits angiogenesis of
human hepatoma cells in vivo by suppressing
VEGF production . -
pubmed
Inter - laboratory differences in
sirolimus results from six
sirolimus testing centers using HPLC tandem mass spectrometry ( LC /
MS /
MS ) . -
pubmed
Leflunomide has been shown to cause cell specific inhibition of
inducible nitric oxide synthase (
iNOS ) activation in animal models . -
pubmed
When
gallic acid purified from rose flowers was used to treat the 9 - month - old male SAM , it not only reinstated the activities of
CAT and
GPx but also significantly reduced the amount of
malondialdehyde (
MDA ) in the liver , brain and kidney . -
pubmed
OBJECTIVES : MRI
T2 hypointensity in
multiple sclerosis (
MS ) gray matter , suggesting
iron deposition , is associated with
physical disability , disease course , lesion load , and
brain atrophy . -
pubmed
HPLC determination of
enoxacin ,
ciprofloxacin ,
norfloxacin and
ofloxacin with photoinduced fluorimetric (
PIF ) detection and multiemission scanning : application to urine and serum . -
pubmed
According to the criterium of Clayton , using 0.05 as false positive and false negative error assurance probabilities , detection limits of 7.3 , 6.0 ,
6.3 and 14.5 ng / mL , for ENO ,
NOR ,
CIPRO and OFLO , respectively , have been found . -
pubmed
In the present work , we diagonalize the Hessian matrix of the polarizability with respect to the vibrational nontotally symmetric normal coordinates , to calculate the nontotally symmetric distortions that produce the maximum breakdown of the
MPP in a series of twenty
polycyclic aromatic hydrocarbons . -
pubmed
Arbuscular mycorrhizal fungal (
AMF ) spore communities were surveyed in a long - term field fertilization experiment in Switzerland , where different amounts of
phosphorus ( P ) were applied to soil . -
pubmed
Well - resolved one - dimensional and two - dimensional NMR spectra of
cytochrome P450 BM-3 reveal shifts upon binding of its substrate ,
N - palmitoylglycine . -
pubmed
Plant signal molecules like
salicylic acid ,
methyl jasmonate ,
ethylene and
spermine , known to be involved in plant defense were not able to induce the investigated genes , therefore , an unknown signalling mechanism is expected to operate in
BR . -
pubmed
CONCLUSIONS : Antioxidant vitamin supplementation during
IFN - alpha-2b and
ribavirin therapy prevented a decrease in
EPA of mononuclear cell phospholipids . -
pubmed
If a further decrease in the ratio of
arachidonic acid to
EPA can be achieved by using oral
EPA supplementation , the efficacy of
IFN - alpha-2b and
ribavirin therapy may be improved . -
pubmed
To investigate mechanisms of the basal activity of AHA
angiotensin II - sensitive neurons , we examined the effect of
N -( 6 - aminohexyl )- 5 - chloro-1 - naphthalenesulfonamide hydrochloride ( W7 ) , a
calmodulin inhibitor , applied onto AHA neurons on the basal activity and the stimulus - evoked activation of these neurons . -
pubmed
Microinjections of
carbachol into the LSV and
corticotropin - releasing factor into the MeA , and intracerebroventricular injection of hypertonic saline , activated AHA
angiotensin II - sensitive neurons . -
pubmed
These three kinds of injection - induced activations of AHA neurons were abolished by pressure application of W7 onto the same neurons , while the
calmodulin inhibitor did not affect the increase in firing of AHA neurons induced by pressure application of
angiotensin II onto the same neurons . -
pubmed
The pressure application of W7 did not affect the basal activity of AHA
angiotensin II - sensitive neurons , whereas the
angiotensin AT1 receptor antagonist
losartan similarly applied inhibited it . -
pubmed
Compared to the control side , the
tetracycline labeling lines were wider ; the increase of the expression level of
BMP-2 appeared earlier , greater and lasted longer in the tension area of the experimental side . -
pubmed
We conclude that the primary cause of increased
estrogen levels in acute illness is increased
aromatase P450 gene expression , resulting in enhanced aromatization of
androgens to
estrogens , a previously undescribed endocrine response to
acute illness . -
pubmed
Binding of
LT - I to cells , when
GM1 was blocked by the
cholera toxin B subunit , was characterized by an increased number of
LT - I receptors with respect to undifferentiated control cells . -
pubmed
Moreover , both types of differentiated cells accumulated higher amounts of
cyclic AMP in response to
LT - I than undifferentiated cells . -
pubmed
Helix pomatia lectin inhibited the binding of
LT - I to cells and the subsequent production of
cyclic AMP . -
pubmed
Associations of body size at birth with late - life
cortisol concentrations and
glucose tolerance are modified by haplotypes of the
glucocorticoid receptor gene . -
pubmed
Moreover , this haplotype modified the association of length at birth with adult phenotypes ; in carriers , short length at birth was associated with increased fasting plasma
cortisol ,
cortisol /
corticosteroid - binding globulin ratio ,
impaired glucose tolerance or
diabetes [ 1 cm decrease corresponded to 1.36 - fold odds ratio ; 95 % confidence interval ( CI ) , 1.09 - 1.70 ; P = 0.007 ] , and higher 120 - min
glucose ( 5.8 % ; 95 % CI , 2.5 - 9.1 % ; P = 0.0007 ) , but no association was seen in noncarriers ( P for interaction was 0.06 , 0.01 , 0.02 , and 0.01 , respectively ) . -
pubmed
CONCLUSIONS : A common
GR haplotype may contribute to and modify the association of short length at birth with adult
glucose tolerance and
HPAA function by a mechanism that affects regulation of
GR expression . -
pubmed
HSE produced a concentration - dependent vasodilation ( IC50 = 10.8 + /- 4.0 microg / mL ) in arterial rings pre - contracted with
phenylephrine , which was completely abolished in endothelium - denuded vessels . -
pubmed
Endothelium - dependent vasodilation induced by
HSE was strongly reduced by
L - NAME ( 100 microM ) , a
nitric oxide ( NO ) synthase inhibitor , but neither by
atropine , a
muscarinic receptor antagonist ( 1 microM ) , nor by
indomethacin ( 10 microM ) , a
cyclooxygenase inhibitor . -
pubmed
In rings pre - contracted with 80 mM
KCl , the vasodilator effect of
HSE was shifted to the right and was completely abolished in the presence of
L - NAME ( 100 microM ) . -
pubmed
In addition ,
BaCl2 ( 1 mM ) dramatically reduced the vasodilation induced by
HSE . -
pubmed
The goal of this study was to determine if prenatal exposure to
cocaine was associated with an increase in the number of excitatory synapses on dendritic spines in layer
II / III of the prelimbic cortex . -
pubmed
This response was suppressed by approximately 50 % when cells were pretreated with the
B1 receptor antagonist
des - Arg9 -[ Leu8 ]- bradykinin while treatment with
des - Arg9 - bradykinin , the
B1 receptor agonist , caused a concentration - dependent increase in
hCXCL5 and
hCXCL6 mRNA expression . -
pubmed
Do
insulin ( or IGF-1 ) and
glucagon act oppositely in controlling eye growth , as they do in metabolic pathways and in control of retinal neurogenesis ? -
pubmed
In eyes without lenses , both
insulin and IGF-1 speeded , and
glucagon slowed , ocular elongation , but
glucagon and
insulin each increased the rate of thickening of the crystalline lens . -
pubmed
When injected together ,
insulin blocked choroidal thickening by
glucagon , at a dose that did not , by itself , thin the choroid . -
pubmed
CONCLUSIONS : Glucagon and
insulin ( or IGF-1 ) cause generally opposite modulations of eye growth , with
glucagon mostly increasing choroidal thickness and
insulin mostly increasing ocular elongation . -
pubmed
Using cells expressing recombinant
alpha3beta4 nAChRs , these molecules inhibited
calcium accumulation ( a more direct assay to establish
nAChR activity ) , with IC(50 ) values ranging from 0.7 to 38.2 microM. -
pubmed
IGF-1 treatment increased
phospho -
Akt fourfold at 30 min ,
cyclins D1 and E by 6 h , and decreased
p27 ( KIP1 ) and
p57 ( KIP2 ) expression . -
pubmed
The biological effects of FGFs are mediated through the activation of the four transmembrane phosphotyrosine kinase receptors (
FGFRs ) in the presence of
heparin sulfate proteoglycans ( HSPGs ) and therefore require the release of FGFs into the extracellular space . -
pubmed
To understand the molecular events in the
FGF-1 releasing pathway , we have studied the interactions of
S100A13 with
C2A by
( 1 ) H -( 15 ) N HSQC titration and 3D - filtered NOESY experiments . -
pubmed
The most promising application is the selective interaction of covalently fixed
boronic acid residues with
glucose , which renders itself not only for sensing , but eventually also for delivery of drugs such as
insulin . -
pubmed
Those taking
TDF and a
PI / r had a greater median decline in
eGFR than those taking
TDF and a non - NRTI at 6 months ( P = 0.01 ) , with trends at 12 ( P = 0.08 ) and 24 months ( P = 0.08 ) . -
pubmed
Our results support the use of
TDF as a component of the initial antiretroviral regimen , and suggest that the
eGFR should be monitored more closely when
TDF is used with a
PI / r. -
pubmed
SUCROSE SYNTHASE (
SUS :
EC 2.4.1.13 ) , a key enzyme in plant
sucrose catabolism , is uniquely able to mobilize
sucrose into multiple pathways involved in metabolic , structural , and storage functions . -
pubmed
A constructed atu2422 mutant was more virulent than the wild type bacterium ; moreover , transgenic plants with a low level of
Pro exhibited less severe
tumor symptoms than did their wild - type parents , revealing a crucial role for
Venus flytrap GABA - receptor and relative abundance of
GABA and
Pro in host - pathogen interaction . -
pubmed
Nicotinic
acetylcholine and
adenosine type 1 and
2A densities were significantly down - regulated in the putamen of
HE brains . -
pubmed
2MeSADP reduced cell proliferation and increased
Caspase-3 activity ; both these effects could be fully reversed by the
P2Y ( 13 ) receptor antagonist
MRS2211 . -
pubmed
These results indicate that
P2Y ( 13 ) is a proapoptotic receptor in beta - cells as the
P2Y ( 13 ) receptor antagonist
MRS2211 is able to protect the cells from
ADP induced apoptosis . -
pubmed
We examined the effects of
interleukin-1 beta (
IL-1beta ) ,
tumor necrosis factor alpha (
TNF - alpha ) , lipopolysaccharide ( LPS ) , lipoteichoic acid (
LTA ) , zymosan (
ZYM ) ,
dexamethasone (
DEX ) ,
cyclosporine , and
tacrolimus on transendothelial electrical resistance ( TEER ) ; endothelial tight junctions ; filamentous
actin ; and permeability to
deoxycholate AMB (
DAMB ) , liposomal
AMB (
LAMB ) , and
fluconazole . -
pubmed
TNF - alpha and LPS increased the permeability to
AMB by 8.2 to 14.5 % compared to that for the controls ( 1.1 to 2.4 % ) ( P & lt ; or = 0.04 ) . -
pubmed
In conclusion ,
IL-1beta ,
ZYM , and
LTA increased the permeability of the BBB to small ions but not to
AMB , whereas
TNF - alpha and LPS , which disrupted the endothelial layer integrity , increased the permeability to
AMB . -
pubmed
Mutation analysis of mitochondrial
12S rRNA gene in Polish patients with non - syndromic and
aminoglycoside - induced
hearing loss . -
pubmed
In the present study , we have performed mutational screening of entire
12S rRNA gene in 250 unrelated patients with non - syndromic and
aminoglycoside - induced
hearing loss . -
pubmed
Moreover , two
12S rRNA sequence variants : m.988 G>A and m.1453 A>G , localized at conserved sites and affected RNA secondary structure , may be new candidates for non - syndromic and
aminoglycoside - induced
hearing loss associated mutations . -
pubmed
AIM : Because of its
zinc - free formulation
insulin glulisine ( GLU ) might have a faster onset of action than other short - acting analogues . -
pubmed
We compared the pharmacokinetics and pharmacodynamics of
GLU with those of
insulin aspart (
ASP ) . -
pubmed
RESULTS : GLU showed a significantly higher early metabolic effect ( area under the
glucose infusion rate (
GIR ) curve in the first 30 min AUC -
GIR ( 0-)30 min 30.3 + - 26.4 vs.
16.2 + - 18.4 mg / kg , P = 0.0421 ) than ASP , an earlier onset of action ( time to 10 % of
GIR ( max ) (
GIR ( max)-t ( 10 % ) ) 9 vs. 17 min , P = 0.0146 ) and a faster absorption ( shorter times to 10 % and 20 % of
INS ( max , ) P = 0.0005 each ) . -
pubmed
Finally , HbA1c testing might be biased due to the interference from several
hemoglobin variants , is characterized by a higher imprecision than blood
glucose measurement , and is more expensive . -
pubmed
We demonstrate that
2G12 is produced with complex N - glycans at great uniformity in the wt as well as in the glycosylation mutant , carrying a single dominant glycosylation species , GnGnXF and
GnGn , respectively . -
pubmed
OBJECTIVE : The substitution of
selenium activates the
selenium - dependent enzyme
glutathione peroxidase , which is important for scavenging free radicals . -
pubmed
Paclitaxel ameliorates
fibrosis in hepatic stellate cells via inhibition of
TGF - beta /
Smad activity . -
pubmed
RESULTS : The
paclitaxel treatment markedly suppressed
Smad2 / 3 phosphorylation . -
pubmed
Self - assembly of the recombinant hydrophobin was followed kinetically using a
thioflavin T fluorescence binding assay , and contact angle measurements of purified recombinant
hydrophobin protein (
mHyd2 ) films on a variety of substrata demonstrated its surface modification ability , which remained stable for at least 4 months . -
pubmed
The
rest of the metal stent was purged with
NaCl 0.9 % . -
pubmed
This
enzyme is likely to be a
cysteine protease ; it was found active in media containing
EDTAK2 and
dithiothreitol , it maintained considerable activity at temperatures 14 degrees C to 33 degrees C and pH 6.50 to 8.50 with optimum k(cat)/Km and/or k(cat ) values at pH 7.00 and 25 degrees C. The activity of
protease - B-17N-1 was strongly affected by the specific irreversible inhibitor of
cysteine proteases E-64 , while it remained unaffected by the
3,4 - dichloro - isocoumarine , an irreversible inhibitor specific for
serine proteases . -
pubmed
Protease - B-17N-1 retained full activity at 25 degrees C after 30 min incubation at 8 degrees C or at 33 degrees C ; moreover , it was found to be stable and active in the polar organic solvents
DMSO and
acetonitrile . -
pubmed
The
enzyme hydrolyzed the substrate
Cbz - FR - pNA via Michaelis - Menten kinetics , while it showed insignificant activity for the substrate
Suc - AAA - pNA . -
pubmed
In addition , interesting results were obtained from the effect of different metallic ions and polar organic solvents on the Michaelis - Menten parameters of
protease - B-17N-1 , showing that it performs catalysis via a
( Cys )- S (-)/( His )- Im (+) H ion - pair , as well as its industrial and biotechnological potential , respectively . -
pubmed
The phagocytic uptake of apoptotic /
necrotic cells involves a plethora of molecules , including immunoglobulins ,
lectins , components of the complement system ( all of which act as opsonins ) , as well as the phospholipid
phosphatidylserine (
PS ) and the endoplasmic reticulum chaperone
calreticulin (
CRT ) , both of which can be exposed on the surface of dying cells . -
pubmed
Recently , this view has been challenged by the observations that , similar to
PS ,
CRT acts as a facultative recognition ligand on apoptotic cells , and that cytotoxic agents such as
anthracyclines induce the exposure of
CRT on the surface of dying
tumor cells , thereby generating an engulfment signal that stimulates the uptake of apoptotic
corpses and the presentation of the corresponding antigens by dendritic cells . -
pubmed
Other neural areas that may interact with this putative circuit include amygdala , ventral hypothalamus , periaqueductal gray (
PAG ) , and deep tectum , as well as ascending
dopamine systems which participate in all types of seeking urges . -
pubmed
In the MD and TD groups ,
FSH injections were begun on the Day 8 of
progestagen treatment . -
pubmed
Database water composition is dominated by
sodium bicarbonate and
sodium chloride type waters with total dissolved solids concentrations of 150 to 39,260 mg / L. Constituents commonly exceeding standards for drinking , livestock , and irrigation water applications were total dissolved solids ( TDS ) ,
sodium adsorption ratio (
SAR ) , temperature ,
iron , and
fluoride . -
pubmed
Targeting
IL5 with
mepolizumab seems promising in sparing
steroids , but
relapses often follow its withdrawal . -
pubmed
The low - frequency ( LF)/high - frequency (
HF ) power ratio , an index of cardiac sympathovagal balance , was significantly lower with
efonidipine and
cilnidipine than with
amlodipine , while the
HF / total power ratio , an index of cardiac vagal activity , revealed the opposite results . -
pubmed
There was no significant correlation between the LF /
HF ratio and plasma
norepinephrine levels . -
pubmed
PURPOSE : The aim of the present study was to investigate plasma concentrations of
Gas6 and its soluble
tyrosine kinase receptor
sAxl in
Systemic lupus erythematosus (
SLE ) and
Behcets disease (
BD ) patients and to correlate those levels with clinical and laboratory manifestations of the diseases . -
pubmed
The
InSb working standard was also measured at the National Physical Laboratory (
NPL ) of the United Kingdom in 1999 . -
pubmed
The less than 2 % spectral power responsivity disagreements obtained on the
InSb working standard ( both from the 1999 NIST and
NPL comparison and also against the pyroelectric standard ) validate the three independently realized power responsivity scales and verify the long - term stability of the
InSb working standard . -
pubmed
We further show that
UHRF1 association with H3K9 methylation is insensitive to adjacent
H3 S10 phosphorylation -- a known mitotic '
phospho -
methyl switch ' . -
pubmed
Accelerated mitochondrial aging induced by
nucleoside reverse transcriptase inhibitors ( NRTIs ) has been implicated as a part of this phenomenon . -
pubmed
Recent studies suggest that
RAS blockers may reduce
inflammation by increasing endogenous concentrations of the anti - inflammatory heptapeptide
angiotensin -( 1 - 7 ) [
Ang -( 1 - 7 ) ] . -
pubmed
Treatment with
U-0126 , a
MEK inhibitor , demonstrates that this radiation - induced
inflammation in astrocytes is mediated through the
MAP kinase pathway . -
pubmed
Ang -( 1 - 7 ) inhibits radiation - induced
inflammation , increases in
PKCalpha , and
MAP kinase pathway activation ( phosphorylation of
MEK and
ERK ) . -
pubmed
Additionally
Ang -( 1 - 7 ) treatment leads to an increase in
dual specificity phosphatase 1 (
DUSP1 ) . -
pubmed
Furthermore , treatment with
sodium vanadate (
Na3VO4 ) , a
phosphatase inhibitor , blocks
Ang -( 1 - 7 ) inhibition of radiation - induced
inflammation and
MAP kinase activation , suggesting that
Ang -( 1 - 7 ) alters phosphatase activity to inhibit radiation - induced
inflammation . -
pubmed
There are many proposed pharmacological treatments including : beta - blockers ,
angiotensin - converting enzyme inhibitor (
ACE inhibitors ) ,
angiotensin - receptor blocker ( ARB ) , statins ,
macrolides and ,
doxycycline , an inhibitor of the
MMP . -
pubmed
The structural and magnetic properties of nanostructured
Co - rich
transition - metal alloys ,
Co(100 - x)TMx (
TM =
Hf ,
Zr and 10 & lt;= x & lt;= 18 ) , were investigated . -
pubmed
PBDE exposures for 96 h have caused
chitobiase and chitinolytic enzymes inhibition . -
pubmed
BACKGROUND : To investigate the antioxidant value and anticancer functions of
mitragynine (
MTG ) and its
silane - reduced analogues (
SRM ) in vitro . -
pubmed
MATERIALS AND METHODS :
MTG and
SRM was analyzed for their reducing power ability ,
ABTS radical inhibition and
1,1 - diphenyl-2 - picryl hydrazylfree radicals scavenging activities . -
pubmed
RESULTS :
SRM and
MTG demonstrate moderate antioxidant value with
ABTS assay (
Trolox equivalent antioxidant capacity ( TEAC ): 2.25 + -0.02 mmol
trolox / mmol and 1.96 + -0.04 mmol
trolox / mmol respectively ) and
DPPH ( IC50=3.75 + -0.04 mg / mL and IC50=2.28 + -0.02 mg / mL respectively ) . -
pubmed
Three variables (
C - reactive protein , total
cholesterol , and instability of total sleep time ) contributed to a combined moderator of faster decrease in
BMI with IRRI treatment . -
pubmed
To assess thyroid function ,
serum thyroid stimulating hormone (
TSH ) , free
thyroxine ( FT4 ) , free
triodothyronine ( FT3 ) and TPOAb /
thyroglobulin antibody (
TGAb ) levels were determined using chemiluminescent immunoassays every 3 mo . -
pubmed
FLNB transcript analysis of the c.2055 G & gt ; C variant revealed insertion of 27 bp intronic sequence between exon 13 and 14 which gives rise to in - frame deletion of
glutamine 685 and insertion of ten novel
amino acid residues ( p. Gln685delins10 ) . -
pubmed
Of untreated SCB about 63 % of the lignin compounds were assigned as
p - coumarates and
ferulates , analysed by pyrolysis -
GC /
MS as
4 - vinyl phenol and
4 - vinyl guaiacol , and designated as non - core lignin (
NCL ) compounds . -
pubmed
The first
sugar of the repeat units is essential for the
Wzy polymerase activity and elongation of the O - antigen lipopolysaccharide . -
pubmed
In the
Wzx /
Wzy - dependent assembled pathway , the assembled
O - antigen repeat units are translocated from the cytosolic to the periplasmic
face of the inner membrane by a
Wzx translocase and then polymerized by the integral membrane protein
Wzy to form a glycan chain . -
pubmed
OBJECTIVE : To prepare the slow - release complex with
rifampicin ( RFP)-
polylactic - co - glycolic acid (
PLGA)-
calcium phosphate cement ( CPC ) (
RFP -
PLGA -
CPC complex ) , and to study its physical and chemical properties and drug release properties in vitro . -
pubmed
: METHODS : The emulsification - solvent evaporation method was adopted to prepare
rifampicin polylactic acid -
glycolic acid ( RFP -
PLGA ) slow - release microspheres , which were divided into 3 groups : a
calcium phosphate bone cement group ( CPC group ) , a CPC embedded with
RFP group (
RFP -
CPC group ) , and a
PLGA slow - release microspheres carrying
RFP and the self - curing CPC group ( RFP-
PLGA -
CPC complex group ) . -
pubmed
: RESULTS : The CPC group showed the shortest solidification time , while the
RFP -
PLGA - CPC complex group had the longest one . -
pubmed
There was statistical difference in the porosity between the CPC group and the RFP - CPC group ( P<0.05 ) ; Compared to the
RFP -
PLGA - CPC complex group , the porosity in the CPC group and the
RFP - CPC group were significantly changed ( both P<0.01 ) . -
pubmed
There was significant difference in the compressive strength between the RFP-
PLGA - CPC complex group and the
CPC group ( P<0.01 ) , while there was significant difference in the compressive strength between the
RFP - CPC group and the
CPC group ( 3 days : P<0.05 ; 30 and 60 days : P<0.01 ) . -
pubmed
The pores of section in the
RFP -
PLGA -
CPC complex group increased obviously , and
PLGA microspheres gradually disappeared until the 60th day when there were only empty cavities left . -
pubmed
The
RFP -
PLGA -
CPC complex group had no obvious drugs sudden release , and the cumulative drug release rate was nearly 95 % in the 60 days . -
pubmed
To isolate MAP , the double incubation method was used for decontaminating the tissues and Middlebrook 7H9 broth - based culture associated with OADC (
oleic acid ,
albumin ,
dextrose , and
catalase ) supplement with / without
mycobactin J were used . -
pubmed
Phase 2 had a greater proportion of
vancomycin courses with therapeutic initial trough concentrations ( 27.5 % vs 46.1 % ; p = 0.002 ) , higher initial trough concentrations ( 10.9 mcg / mL vs 16.4 mcg / mL ; p & lt ; 0.001 ) , and optimized initial
vancomycin dosing ( 13.5 mg / kg vs
16.2 mg / kg ; p & lt ; 0.001 ) . -
pubmed
Severe
hypertriglyceridemia is defined at a plasma
triglyceride (
TG ) concentration of & gt;885 mg / dl and may result - in particular when clinical symptoms appear before the age of 40 - from " large variant " mutations in genes which influence the function of the
lipoprotein lipase (
LPL ) . -
pubmed
Omega-3 - Fatty acids and
fibrates can be used to lower plasma
TG levels . -
pubmed
The structures of
K11 and
K83 CPS produced by isolates LUH5545 and LUH5538 , which carry related
KL11a and
KL83 gene clusters , respectively , were established by
sugar analysis and one- and two - dimensional 1H and
13C NMR spectroscopy . -
pubmed
Both
CPS contain
l - rhamnose (
l - Rha ) and
6 - deoxy - l - talose (
l-6dTal ) , and both
KL gene clusters include genes for
dTDP - l - Rhap synthesis and a
tle ( talose epimerase ) gene encoding an
epimerase that converts
dTDP - l - Rhap to
dTDP - l-6dTalp . -
pubmed
The K11 and
K83 repeat units are the same
pentasaccharide , consisting of
d - glucose ,
l - Rha ,
l-6dTal , and
N - acetyl - d - glucosamine , except that
l-6dTal is 2 - O - acetylated in
K83 . -
pubmed
However , the K units are linked differently , with
l - Rha in the main chain in K11 , but as a side - branch in
K83 . -
pubmed
Combined assessment of
DYRK1A ,
BDNF and
homocysteine levels as diagnostic marker for
Alzheimer ' s disease . -
pubmed
Receiver - operating characteristic curves and logistic regression analyses showed that combined assessment of
DYRK1A ,
BDNF and
homocysteine has a sensitivity of 0.952 , a specificity of 0.889 and an accuracy of 0.933 in testing for
AD . -
pubmed
RESULTS : The ratio of partial pressure of arterial
oxygen to the fraction of inspired
oxygen only dropped markedly from before
CPB in the control group ( P & lt ; .001 ) . -
pubmed
Dioscin Induces
Gallbladder Cancer Apoptosis by Inhibiting ROS - Mediated
PI3K /
AKT Signalling . -
pubmed
Western blot analysis showed that
AKT activity was significantly downregulated after
DSN treatment , and that inhibition / ectopic expression of
AKT enhanced / abolished
DSN - induced apoptosis but not migration . -
pubmed
Furthermore , we confirmed the relationship between ROS and the
PI3K /
AKT pathway and found that
DSN induced apoptosis by regulating ROS - mediated
PI3K /
AKT signaling . -
pubmed
Taken together , these findings indicate that
DSN induces GBC apoptosis through inhibiting ROS - mediated
PI3K /
AKT signalling . -
pubmed
Efficacy and safety of
proprotein convertase subtilisin - kexin type 9 (
PCSK9 ) inhibitors ,
alirocumab and
evolocumab , a
post - commercialization study . -
pubmed
These data suggest that
19H3 is the preferable antibody to use to detect
4 - 1BBL in the presence of its receptor . -
pubmed
Transmission electron microscopy (
TEM ) revealed that
PLGA NPs and GNPs were spherically shaped , but GNPs had smooth surface while
PLGA NPs had a capsular morphology with a wrinkled surface and large voids . -
pubmed
The results of
TEM and the redshift spectrum demonstrated that GNPs successfully bound to the surface of the
PLGA NPs . -
pubmed
The
sugar chain - binding properties of the
scFv was evaluated by array - type
Sugar Chip immobilized with a library of synthetic glycosaminoglycan
disaccharide structures . -
pubmed
Highly sulphated
disaccharide structures were found to have high affinity to
scFv . -
pubmed
Aimed to unravel their conformational properties we have performed a series of molecular dynamics simulations of Chitosan / Chitlac decamers , exploring different degrees of substitution (
DS ) of
lactitol side chains . -
pubmed
[
Sar1 ,
Ile8]
AngII interacts with both the ' core ' binding domain , where the small - molecule ligands of
AT1R and
AT2R bind , and the ' extended ' binding domain , which is equivalent to the allosteric modulator binding site of
muscarinic acetylcholine receptor . -
pubmed
Stereoselective Double Reduction of
3 - Methyl-2 - cyclohexenone , by Use of
Palladium and
Platinum Nanoparticles , in Tandem with
Alcohol Dehydrogenase . -
pubmed
The combination of metal nanoparticles (
Pd or
Pt NPs ) with
NAD - dependent thermostable
alcohol dehydrogenase (
TADH ) resulted in the one - flask catalytic double reduction of
3 - methyl-2 - cyclohexenone to
3 -( 1S,3S )- methylcyclohexanol . -
pubmed
Resuming , the chemocatalysts were very sensitive to deactivation , which was not related to the amount of
enzyme used , while the inhibition of the biocatalyst can be strongly reduced by minimizing the NPs /
TADH ratio used to catalyze the reaction . -
pubmed
The as - prepared
NiFe / GO hybrid was characterized by transmission electron microscopy (
TEM ) and X - ray diffraction ( XRD ) . -
pubmed
AIM : This randomized controlled cross - over - design - study investigated short - term effects on oral malodour of a
zinc -
lactate - containing mouthwash . -
pubmed
It has already been shown that
CK isoforms play an important role in energy homeostasis by catalyzing a reversible transfer of the
phosphate of
ATP to
creatine to yield
ADP and
creatine phosphate (
CP ) (
creatine /
CP circuit ) . -
pubmed
Histone deacetylase 6 (
HDAC6 ) primarily catalyzes the removal of
acetyl group from the side chain of acetylated
lysine residues in
cytoplasmic proteins such as
alpha - tubulin and
HSP90 . -
pubmed
We herein report our new generation of multifunctional
HDAC6 degraders by tethering selective
HDAC6 inhibitor
Nexturastat A with
CRBN ligand that can synergize with
HDAC6 degradation for the antiproliferation of
multiple myeloma (
MM ) . -
pubmed
Phosphorylated
claudin-16 interacts with
Trpv5 and regulates transcellular
calcium transport in the kidney . -
pubmed
Together , these results suggest a pathogenic mechanism for
FHHNC involving transcellular
Ca2 + pathway in the DCT and identify a molecular component in renal
Ca2 + homeostasis under direct regulation of
PTH . -
pubmed
PbSe materials are typically characterized with impact ionization which effectively induces carrier multiplication ( CM ) in the quasi Type -
II PbCdSe composite , exhibiting reasonably high
PCE of 6.14 % with current output of 19.2 mA cm-2 for the optimal
PbCdSe film . -
pubmed
And lipopolysaccharide - induced neuroinflammatory state resulted in significant
depression - like behaviors , including reduced locomotor activity in the open field test , reduced
saccharin preference , added immobility time in tail suspension test and forced swimming test , decreased comb time in the splash test , and increased latency to food in the novelty suppressed feeding test time , and reduced the levels of
neurotrophic factors and synaptic proteins , and decreased Nissl bodies . -
pubmed
Aloin Preconditioning Attenuates
Hepatic Ischemia /
Reperfusion Injury via Inhibiting
TLR4 /
MyD88 /
NF - kappaB Signal Pathway In Vivo and In Vitro . -
pubmed
Results : We found that 20 mg / kg was the optimum concentration of
aloin for mitigating I / R - induced
liver tissue damage , characterized by decreased serum
alanine aminotransferase (
ALT ) and
aspartate aminotransferase (
AST ) . -
pubmed
Moreover ,
aloin inhibited hepatocyte apoptosis and inflammatory response that was caused by the upregulated expression of
Bcl-2 , the downregulated expression of
cleaved caspase3(
C - caspase3 ) ,
Bax ,
Toll - like receptor 4 (
TLR4 ) ,
FADD ,
MyD88 ,
TRAF6 ,
phosphorylated IKKalpha / beta (
p - IKKalpha / beta ) , and phosphorylated
nuclear factor kappaB p65 (
p - NF - kappaB p65 ) . -
pubmed
Oxygen Saturation ( SaO2 % ) was assessed at baseline and after exercise , while
RPE and HR were measured each 10 min during the trial . -
pubmed
The Area Under the Curve ( AUC ) of
RPE was lower in
hypoxia +
CHO compared to
hypoxia . -
pubmed
The AUC of the HR /
RPE ratio in the
hypoxia +
CHO group was higher in relation to
hypoxia . -
pubmed
CONCLUSIONS : Our results indicate that
CHO supplementation does not change
RPE induced by 60 min of exercise at 50 % VO2peak in
hypoxia equivalent to 4200 m at the different times analyzed . -
pubmed
However , in
hypoxia +
CHO the ( AUC)-60 min of total
RPE decreased during exercise , while the heart rate /
RPE ratio improved , indicating lower
RPE in the hypoxic environment . -
pubmed
Cognitive performance as well as biochemical markers [ (
interleukin-16 (
IL-16 ) and
tumor necrosis factor- (
TNF- ) ] levels , reactive
oxygen species ( ROS ) production ,
synaptophysin and
glutamate levels ) have been evaluated at the end of
um - PEA treatment . -
pubmed
However , it remains poorly defined that whether
icariin could inhibit
ER stress and
NF - kappaB mediated apoptosis in
asthma and further influence the central neural system . -
pubmed
We found that
icariin could remarkably improve
pulmonary function and reduce inflammatory cells in the lung , levels of
inflammatory cytokines , and
ER stress related proteins as well as
NF - kappaB were prominently suppressed by
icariin . -
pubmed
Carbon export from leaves is controlled via ubiquitination and phosphorylation of
sucrose transporter SUC2 . -
pubmed
In most plants , photosynthetically produced
sucrose is the predominant
carbon and energy source , whose transport from leaves to
carbon sink organs depends on
sucrose transporters . -
pubmed
An in vivo assay based on a fluorescent
sucrose analog confirmed that
SUC2 phosphorylation by
WAKL8 can increase transport activity . -
pubmed
CUDC-101 , a poorly water soluble multi - targeted anti -
cancer drug that simultaneously inhibits the
receptor tyrosine kinases (
RTKs )
EGFR and
HER2 , as well as
histone deacetylase (
HDAC ) , was encapsulated in polymeric Molecular Envelope Technology (
MET ) NPs . -
pubmed
Polymer
MET nanoparticles coated with
hyaluronidase enabled the subcutaneous delivery of a hydro - phobic drug with favourable therapeutic outcomes . -
pubmed
Clinical pregnancy rate ,
miscarriage rate and healthy baby rate were compared between the MALBAC - NGS -
PGT - A and
MDA - SNP -
PGT - A groups . -
pubmed
The results provide greater evidence supporting the wider use of NGS in
PGT - A , not only for its lower cost but also for its improved clinical outcomes compared to
SNP - based
PGT - A. -
pubmed
We synthesized 55 analogs based on the chemical structure of
4 - gingerol and evaluated their
RhlR inhibitory activities using cell - based reporter
strain assay . -
pubmed
Comprehensive structure - activity relationship studies identified the
alkynyl ketone 30 as the most potent
RhlR antagonist . -
pubmed
Essential role of
calcium in extending
RTX adhesins to their target . -
pubmed
However , all the tetra - tandemers have at least one
calcium ion coordinated in the linker region between beta - sandwich domains whose role appears to be the rigidification of the extender region to help the
adhesin extend its reach . -
pubmed
METHODS : We used a national sample of administrative claims data to explore associations diagnostic test choice (
TST , IGRA ,
TST with subsequent IGRA ) and treatment persistence and completion in persons initiating a daily dose
isoniazid LTBI treatment regimen in the US private healthcare sector between July 2011 and March 2014 . -
pubmed
( Aza ) Acenes Share the
C2 Brink with ( Anti)Aromatic Macrocycles - Local vs. -
pubmed
Background : The Attain Stability Quadripolar 4798 lead is a relatively new quadripolar active fixation coronary
sinus (
CS ) lead . -
pubmed
Serum
cholesterol correlated poorly with
PBI ( r = --0,4427 ) , better correlation was achieved after logarithmic transformation of
PBI values ( r = --0,6839 ) . -
pubmed
Reference line and covariance between log
PBI and serum
cholesterol in decreasing thyroid function did not differ significantly from reference line and covariance in steady state , showing that serum
cholesterol accompanies closely and without greater delay the changing
PBI . -
pubmed
Isoprenaline and
dopamine also increased
phosphorylase a activity . -
pubmed
Since both agents stimulate
adrenergic beta - receptors in cardiac muscle , the results are concordant with the hypothesis that
cAMP is involved as a mediator of the inotropic response to adrenergic beta - stimulation . -
pubmed
The
enzyme activity was inhibited by reduction with
borohydride in the presence of the substrate and was inactivated by
carboxypeptidase A [
EC 3.4.12.2 ] digestion . -
pubmed
Extensive homology in various properties of the
enzyme was observed when it was compared with
enzymes from other sources , though some differences could be found in the
amino acid composition and in the kinetic properties . -
pubmed
The increase in blood pressure could be abolished by intraventricular administration of
saralasin , a blocker of
angiotensin II receptors . -
pubmed
Several single
amino acid substitutions in the
COOH terminus of
IL-6 were found to decrease biological activity significantly . -
pubmed
It is concluded that the amphipathical alpha - helix structure in the
COOH terminus of
IL-6 is critical for ligand receptor interaction . -
pubmed
Plasma
metallothionein concentrations may prove useful for identifying poor
zinc status . -
pubmed
However , since hepatic
metallothionein concentrations rise in response to stress , plasma
metallothionein concentrations are increased in this state when the plasma
zinc concentrations are also likely to be low . -
pubmed
Thus , measurement of both plasma
metallothionein and plasma
zinc concentrations could differentiate a low plasma
zinc due to
dietary zinc deficiency from a low concentration due to stress ,
infection , or other metabolic conditions . -
pubmed
4 - Ipomeanol is a naturally occurring toxin that induces lesions in the lung following its activation to an alkylating metabolite by the pulmonary
cytochrome P-450 system . -
pubmed
These results suggest that the isozymes of pulmonary
cytochrome P-450 that act in concert to metabolize
4 - ipomeanol are relatively insensitive to inhibition by
carbon monoxide . -
pubmed
beta - Adrenergic receptor binding , using
[ 3H ] dihydroalprenolol as the ligand , was saturable , linear , and stereospecific . -
pubmed
More rapid maturation of pulmonary
beta - receptors and
SAM flux in females may be a factor in the female advantage with regard to pulmonary surfactant maturation and the survival of premature neonates . -
pubmed
In addition , they suggest that the activated
Con A receptors generate very similar signals in the two cell types , and that any perturbations of primary signal transduction to the secondary
phosphoinositide and [
Ca2 +]i responses in the S49 phenotype are quantitative rather than qualitative . -
pubmed
The ligand binding site of this receptor undergoes denaturation during solubilization in
digitonin ,
Triton X-100 , or
3 -[( 3 - cholamidopropyl ) dimethylammonio ]- 1 - propanesulfonate at detergent concentrations above 0.1 % , which is evident from the loss of high - affinity binding of
[ 3H ] PK11195 , a ligand selective for the
mitochondrial benzodiazepine receptor . -
pubmed
Ethanol - induced
phospholipase C activation is inhibited by
phorbol esters in isolated hepatocytes . -
pubmed
Ethanol causes a transient activation of the
phosphoinositide - specific phospholipase C in intact hepatocytes and mimics the action of receptor - mediated agonists [ Hoek , Thomas , Rubin & amp ; Rubin ( 1987 ) J. Biol . -
pubmed
Preincubation of the hepatocytes with
phorbol esters which activate
protein kinase C prevented this effect of
ethanol :
phorbol ester treatment inhibited the
ethanol - induced
phosphorylase activation , the increase in intracellular free
Ca2 + concentrations measured in
quin 2 - loaded hepatocytes , and the changes in concentrations of
inositol phosphates ,
phosphoinositides and
phosphatidic acid . -
pubmed
Phorbol esters acted in a concentration range where they activate
protein kinase C ;
phorbol esters that do not activate
protein kinase C were not effective in inhibiting the effects of
ethanol . -
pubmed
The inhibition of
ethanol - induced
Ca2 + mobilization by
phorbol esters was prevented by preincubating the cells with the
protein kinase C inhibitors
1 -( 5 - isoquinolinesulphonyl )- 2 - methylpiperazine ( H7 ) and
sphingosine . -
pubmed
H7 also enhanced the
Ca2 + mobilization induced by
ethanol in cells that were not pretreated with
phorbol esters , indicating that the transient nature of the
ethanol - induced
Ca2 + mobilization may be due to an activation of
protein kinase C caused by the accumulation of
diacylglycerol . -
pubmed
These data support a model whereby
ethanol activates the
phosphoinositide - specific phospholipase C , possibly by affecting receptor -
G - protein - phospholipase C interactions in the membrane . -
pubmed
Angiotensin II ( 100 ng ) , injected into the preoptic area , stimulated the intake of water but not salt , to a lesser extent and with a shorter duration as compared with that induced by
renin ( 5 mU ) , injected into the preoptic area . -
pubmed
This
angiotensin II - induced intake of water was markedly inhibited by
[ Sar1 , Ile8 ]- angiotensin II , an
angiotensin II receptor antagonist , but not by
captopril , an
angiotensin - converting enzyme inhibitor , previously administered through the same cannula . -
pubmed
The effect of
renin was inhibited by
[ Sar1 , Ile8 ]- angiotensin II ( 10 micrograms ) and was eliminated by
captopril ( 25 micrograms ) injected into the preoptic area . -
pubmed
This effect of
renin was not influenced by the peripheral administration of
captopril . -
pubmed
The effect of
renin was also inhibited by
GABA ,
muscimol or
taurine injected into the cerebroventricle , in larger doses , or into the preoptic area in smaller doses. -
pubmed
When tissues were cultured in medium without
progesterone , the amounts of
dPRL measured in the medium declined steadily over 6 days , regardless of the gestational age of the endometrium . -
pubmed
dPRL was consistently measured in medium harvested from cultures that received either
progesterone or
medroxyprogesterone ; however ,
progesterone did not induce an increase in the amounts of
dPRL released by cultures prepared from early pregnancy endometrium . -
pubmed
Experiments were carried out with growing cultures , and the following pathways were demonstrated for metabolism of the growth substrates : ( i )
TMBA produced
GA , which was further degraded without the formation of aromatic intermediates ; ( ii )
GA formed
pyrogallol , which was stable to further transformation ; and ( iii ) CV was degraded by a series of steps involving de - O - methylation , oxidation of the
aldehyde group , and decarboxylation to
3 - chlorocatechol before ring cleavage . -
pubmed
Plasma levels of
cholesterol ,
high - density lipoprotein (
HDL )
cholesterol and
triglycerides did not differ between the two groups . -
pubmed
They also indicate that antidepressant neuroleptics , which are potent
serotonin 5 - HT2 and alpha 1 - adrenergic blocking agents , after chronic administration affect the
serotonin system in a manner similar to that produced by chronic treatment with classical antidepressant drugs . -
pubmed
During primary
infections DTH responses to
leishmanin antigen were detected as early as 8 weeks
PI , and continued up to 27 weeks
PI . -
pubmed
Dopamine , an endogenous
catecholamine , was used alone and in conjunction with selective blockade or adrenergic and
dopaminergic receptors . -
pubmed
The effect was significantly blocked by the peripherally acting dopaminergic blocker
domperidone and by
cis - flupenthixol , which blocks both peripheral and central
dopaminergic receptors . -
pubmed
The effect of
dopamine was not significantly altered by the
beta 1 - adrenoceptor blocker
practolol , the
alpha - adrenoceptor blocker
phentolamine , or the
alpha + beta - adrenoceptor blocker
labetalol . -
pubmed
Consequently , this study indicates that
dopamine acts on gastric antral motility through
dopaminergic receptors . -
pubmed
Hyper(
ADP -
ribosyl)ation of
histone H1 . -
pubmed
Electrophoresis of [
32P]
ADP - ribosylated
histones on first - dimension acid -
urea or acid -
urea -
Triton gels and on second - dimension acid --
urea --
cetyltriammonium bromide gels revealed that , of all
histones , only histone
H1 could be significantly
poly ( ADP - ribosyl )ated while only minimal modification could be recovered with histone
H1(0 ) . -
pubmed
Furthermore , the extent of
ADP - ribosylation present on pancreatic histone
H1 is shown to proportionally retard this protein 's electrophoretic mobility in all gel systems and to consist of a distinct series of at least 12 modification intermediates which can be evidenced , in nuclei or nucleosomes , and fully recovered along with
histone H1 upon its selective extraction with 5 %
perchloric acid . -
pubmed
The generation of these increasingly
ADP - ribosylated forms of
histone H1 is also demonstrated to be time dependent and the more complex
ADP - ribosylated forms of this
histone are favored at high
NAD + concentrations . -
pubmed
The reflex
bradycardia elicited by central vagal nerve stimulation was accentuated by
urapidil given intracisternally , but this only occurred in the presence of
beta adrenoceptor blockade with
propranolol . -
pubmed
Independently from methods ,
acid phosphatase as well as urine
hydroxyprolin show elevated levels only in cases of metastatic formation . -
pubmed
A significant positive relation between
alcohol consumption and
lung - cancer incidence was also found , accounted for primarily by an increased risk among subjects who consumed larger amounts of wine or whiskey , as compared with the risks among nonconsumers of these beverages ( relative risk ,
2.19 , [ P = 0.03 ] and 2.62 [ P less than 0.01 ] , respectively ) . -
pubmed
Lymphokine supernatants induced increased
methyl-3H - TdR incorporation in adherent cells in 3 of 8 experiments , and microscopic proliferation of phagocytic cells was evident in one experiment . -
pubmed
Promoter activity of the gene encoding the
beta - amyloid precursor protein is up - regulated by growth factors ,
phorbol ester ,
retinoic acid and
interleukin-1 . -
pubmed
We analyzed the effects of
nerve growth factor (
NGF ) ,
basic fibroblast growth factor (
bFGF ) ,
phorbol 12 - myristate 13 - acetate (
PMA ) ,
interleukin-1 (
IL-1 ) and
retinoic acid ( RA ) on promoter activity of the beta
APP gene . -
pubmed
The treatment of cells with either
NGF or
bFGF resulted in a higher degree of stimulation in the basal level of promoter activity than when cells were treated with either
PMA ,
IL-1 or RA . -
pubmed
Heme - protein interactions in
horse heart myoglobin at neutral pH and exposed to acid investigated by time - resolved fluorescence in the pico- to nanosecond time range . -
pubmed
The 4822 - ns lifetime was assigned to reversibly
heme - dissociated
myoglobin . -
pubmed
These analyses revealed the presence of three species originating from
heme - protein interactions : the native form of crystalline
myoglobin , the conformation with disorder hemes , and the reversibly dissociated
heme - free
myoglobin . -
pubmed
Labeling with anti -
PCD6 subunit ( of the
inositol trisphosphate / ryanodine receptor ) was demonstrated on carotenoid droplets suggesting they possess
calcium channels . -
pubmed
Subjects have elevated serum
thyroid hormone levels in association with a nonsuppressed
TSH . -
pubmed
Failure to recognize the inappropriate persistence of
TSH secretion , in spite of elevated
thyroid hormone levels , has commonly resulted in erroneous diagnosis leading to treatment aimed to normalize the
thyroid hormone level . -
pubmed
To demonstrate promoter activity , a segment containing 611 bp of the promoter region ( from -442 to +169 ) was subcloned in front of a promoterless
chloramphenicol acetyltransferase (
CAT ) gene . -
pubmed
Alcoholysis of
epsilon - decalactone with
polyethylene glycol - modified
lipase in
1,1,1 - trichloroethane . -
pubmed
These results were discussed in relation to
carbon number of
n - alcohol , optimum temperature and comparison with modified and non - modified
lipases . -
pubmed
Streptomycin - dependent ( Str - D ) ribosomes almost did not translate the
apr mRNA . -
pubmed
Overall fat supplementation did not affect digestibilities of DM ,
ADF , or
NDF but decreased digestibility of
fatty acids . -
pubmed
Tallow ,
Ca salt of
palm fatty acids , and prilled
fatty acids did not differ in milk yield response . -
pubmed
It was found that the group receiving
EPN ( n = 46 ) normalized their
nitrogen balance sooner and demonstrated a consistent decrease in their
creatine phosphokinase level . -
pubmed
Enhancement of
IL-13 production by
cyclosporin A. -
pubmed
In this work , we show that
IL-13 is produced optimally by T cells when stimulated with a combination of
anti - CD28 and
PMA . -
pubmed
The effect of
CsA did not depend on the presence of
PMA ; upon
CD3 and
CD28 stimulation ,
CsA equally enhanced
IL-13 production . -
pubmed
Both naive and memory
CD4 + T cells and
CD8 + T cells produced
IL-13 , and production in all cell types could be enhanced by
CsA. -
pubmed
In contrast to
IL-13 ,
IL-4 production was observed mainly in
CD4 + memory cells , required costimulation through
CD3 , and was inhibited by
CsA. -
pubmed
The finding that
IL-13 production is enhanced instead of diminished in the presence of
CsA may explain the Th2 - inducing effects of
CsA in vivo . -
pubmed
Transfections of antisense
oligonucleotides or antisense cDNA plasmids into these cell lines using dendrimers resulted in a specific and dose dependent inhibition of
luciferase expression . -
pubmed
Burial of the hydrophobic
amino acids on trimer formation is a strongly conserved feature in the
vicilin family . -
pubmed
Because the
neurotoxicity induced by activation of the
NMDA subtype of
glutamate receptor is mediated through excessive
Ca2 + influx , we investigated whether
SRIF counteracted
NMDA - induced neuronal cell
death . -
pubmed
SRIF type 2 receptor agonists ,
octreotide (
SMS 201 - 995 ; 10 microM ) and
vapreotide (
RC 160 ; 10 microM ) , did not influence the
NMDA - dependent
neurotoxicity . -
pubmed
Pertussin toxin ( 300 ng / ml ) , a
G protein blocker , antagonized the protective effect of
SRIF on
NMDA neurotoxicity . -
pubmed
Furthermore , the neuroprotective effect of
SRIF was mimicked by
dibutyryl - cyclic GMP ( 10 microM ) , a
cyclic GMP analogue , whereas
8 -( 4 - chlorphenylthio )- cyclic AMP ( 10 microM ) , a
cyclic AMP analogue , was ineffective . -
pubmed
The
cyclic GMP content was increased in a dose - dependent manner by
SRIF ( 2.5 - 10 microM ) . -
pubmed
Finally , both specific (
Rp-8 - bromoguanosine 3 ' , 5 '- monophosphate , 10 microM ) and nonspecific [
1 -( 5 isoquinolinylsulfonyl )- 2 - methylpiperazine ( H7 ) , 10 microM ]
cyclic GMP - dependent protein kinase (
cGMP - PK ) inhibitors did not interfere with
NMDA toxicity but substantially reduced
SRIF neuroprotection . -
pubmed
Our data suggest a selective neuroprotective role of
SRIF versus
NMDA - induced nonapoptotic neuronal
death in cortical cells . -
pubmed
This effect is likely mediated by
cGMP - PK presumably by regulation of the intracellular
Ca2 + level . -
pubmed
LDL was isolated and susceptibility of
LDL to
V-70 ( 4 - methoxy-2,4 - dimethylvalerinitrile )-mediated oxidation was assessed by measuring
conjugated diene production at 234 nm , the lag phase being a measure of the resistance of
LDL to oxidation . -
pubmed
Alveolar neutrophil oxidative burst and
beta2 integrin expression in experimental acute
pulmonary inflammation are not modified by inhaled
nitric oxide . -
pubmed
We found that 18 h after LPS ,
lung wet , dry , and wet - to - dry weights ,
H2O2 production , and
CD11b / CD18 expression were increased . -
pubmed
PMN obtained from
BAL were highly activated as evidenced by an already maximal expression of the
beta2 integrin CD11b / CD18 , whereas the high
H2O2 production at basal state could be further enhanced after ex vivo stimulation . -
pubmed
DNA isolated from intact mitochondria treated with
DNase consisted of 16.3 kilobase pairs , mostly circular , mitochondrial DNA molecules and a mixture of nuclear and mitochondrial DNA fragments , as identified by
agarose gel electrophoresis and hybridization . -
pubmed
Effects of mitochondria and
o - methoxybenzoylalanine on
3 - hydroxyanthranilic acid dioxygenase activity and
quinolinic acid synthesis . -
pubmed
The use of
o - methoxybenzoylalanine , a selective
kynureninase inhibitor , has been proposed with the aim of reducing brain synthesis of
quinolinic acid , an excitotoxic
tryptophan metabolite . -
pubmed
In liver homogenates , however , this compound caused unexpected accumulation of
3 - hydroxyanthranilic acid , the product of
kynureninase activity and the precursor of
quinolinic acid . -
pubmed
To explain this observation , we investigated the interaction(s ) of
o - methoxybenzoylalanine with
3 - hydroxyanthranilic acid dioxygenase , the enzyme responsible for
quinolinic acid formation . -
pubmed
When the purified
enzyme or partially purified cytosol preparations were used ,
o - methoxybenzoylalanine did not affect
3 - hydroxyanthranilic acid dioxygenase activity . -
pubmed
It is interesting that addition of purified mitochondria to
3 - hydroxyanthranilic acid dioxygenase preparations reduced the enzymatic activity and the synthesis of
quinolinic acid . -
pubmed
Cimetidine activates
interleukin-12 , which enhances cellular immunity . -
pubmed
IIR was activated by hyperpolarisation below the K+ equilibrium potential and could be blocked by submillimolar concentrations of
Cs + or
Ba2 + , and partially by physiological concentrations of
Na + . -
pubmed
Several posttranslational modifications of subunits
II , III , and
IV were observed : ( 1 ) N - terminal
methionines of subunits III and
IV are split off . -
pubmed
PHP is divided into types Ia , b , c and
II , depending on the presence of
Albright ' s hereditary osteodystrophy (
AHO ) , defective urinary excretion of
phosphate ( U - P ) and response in urinary excretion of cyclic adenosine monophosphate ( U - cAMP ) after the administration of exogenous PTH . -
pubmed
The dependence of the expression of the tagged loci on the
FixL /
FixJ oxygen - sensing two - component regulatory system was examined . -
pubmed
The remaining seven loci showed
fixL- and
fixJ - independent induction of expression in response to
oxygen limitation . -
pubmed
Permeabilized platelets , expressing normal levels of
P - selectin , displayed a
Ca ( 2 +)-dependent increase in shape change and
MLC - P. -
pubmed
Thrombin activated
RhoA as measured by a
rhotekin - binding assay within 30 s of stimulation under conditions of constant [
Ca ( 2 +)]( i ) . -
pubmed
Under the same conditions and timecourse ,
thrombin or GTPgammaS induced an increase in
MLC - P and platelet shape change which was not dependent on an increase in [
Ca(2+)](i ) . -
pubmed
The
thrombin- and GTPgammaS - induced
MLC - P in constant [
Ca ( 2 +)]( i ) was inhibited by the addition of
Y27632 , a
Rho - kinase inhibitor . -
pubmed
This study directly demonstrates that
thrombin can activate
RhoA in platelets in a timecourse compatible with a role in increasing
MLC - P and shape change ( not involving an increase in [
Ca(2+)](i ) ) . -
pubmed
Fructose feeding resulted in
hyperinsulinemia and elevated blood pressure when compared with that in controls ( plasma
insulin , 311.3+/-11.4 v control 164.4+/-11.8 pmol / L , P & lt ; .05 ;
SBP , 135.4+/-4.2 v control 105.5+/-1.3 mm Hg , P & lt ; .05 ) . -
pubmed
Therefore , the antihypertensive effect of
L - arginine could be , at least in part , the result of the restoration of plasma
insulin levels by its vasodilator ability to increase blood flow to
insulin sensitive tissues . -
pubmed
FAK antisense
oligodeoxynucleotides ( ODNs ) were transfected into SMCs by cationic lipid to investigate its modulatory effects on
tyrosine phosphorylation . -
pubmed
Three types of interventions were found : technical interventions in the dialysis mechanisms ( increasing the frequency of dialysis sessions and varying the concentration of
sodium in the dialysate ) , pharmaceutical interventions (
ACE - inhibitors ) , and a dietetic intervention . -
pubmed
AIM : To investigate factors affecting the properties of antisense
oligodeoxy nucleotides ( ASON)-liposomes complex and their cellular uptake . -
pubmed
All of the drugs promote reductions in the levels of
cytochromes b and c in the cells , whilst the
Ag ( I ) complex also lowers the amount of
cytochrome aa3 . -
pubmed
Interestingly , neither the treatment with
dexamethasone in vitro nor triggering of apoptosis in vivo up- regulated
clusterin expression , opposing the view that
clusterin is involved in apoptotic processes . -
pubmed
Omapatrilat inhibits both
angiotensin - converting enzyme (
ACE ) and
neutral endopeptidase (
NEP ) . -
pubmed
Both processes affect the abundance of volatile organic compounds ( VOCs ) in the breath because oxidative stress causes lipid peroxidation of
polyunsaturated fatty acids in membranes , producing
alkanes and
methylalkanes which are catabolized by
CYP . -
pubmed
Breath samples were analyzed by gas chromatography and mass spectroscopy in order to determine the breath
methylated alkane contour (
BMAC ) , a three - dimensional display of the alveolar gradients ( abundance in breath minus abundance in room air ) of
C4 - C20 alkanes and
monomethylated alkanes . -
pubmed
The
chaperone mini -
GroEL is a soluble recombinant fragment containing the 191 - 345
amino acid sequence of
GroEL with a 6xHis tag . -
pubmed
In a suspended system , mini -
GroEL showed significant enhancement of the activity recovery of
rhIFN - gamma , applyed with a 1 - 5:1 stoichiometry of mini -
GroEL to
rhIFN - gamma at 25 degrees C. Moreover , 1 M
urea in the renaturation buffer had a synergistic effect on suppressing the aggregation and improving the activity recovery . -
pubmed
Measurements included cell recovery , platelet viability according to hypotonic
shock response (
HSR ) ,
platelet aggregation with
ADP , morphological and ultrastructural properties of defrozen platelets . -
pubmed
Apoptosis of these cells caused by
Phx was unlikely to be associated with
p53 ,
Bax , and
Bcl-2 , because the levels of these proteins were not altered regardless of the presence or absence of
Phx . -
pubmed
RESULTS : Two known genes
SLC6A1 and
SLC6A11 were identified ; as well as the
GC content of this genomic sequence was 47 % and 3 putative
CpG islands were predicted in the genomic sequence , located in 130,685 - 131,516 bp , 307,090 - 307,870 bp and 415,585 - 416,308 bp , respectively . -
pubmed
CONCLUSIONS : The methods , as mentioned above , might be used for annotating the biological information in the genomic sequence , such as gene structure ,
GC content ,
CpG island . -
pubmed
OCCUPANCY OF
ADRENERGIC RECEPTORS AND INHIBITION OF
CATECHOL O - METHYL TRANSFERASE BY
TROPOLONES . -
pubmed
INTERACTION OF
PHOSPHOROTHIOATE WITH THE
DISULFIDE BONDS OF
RIBONUCLEASE AND LYSOZYME . -
pubmed
Laser Doppler perfusion ratio ( R / L ) of ligated versus nonligated distal hindlimb increased from 0.06 + /- 0.01 ( immediately after ligation ) to 0.25 + /- 0.02 ( day 7 ) in the
PBS group and only from 0.07 + /- 0.02 to 0.13 + /- 0.02 in the
mibefradil group ( p & lt;.01 ) . -
pubmed
Relative
hemoglobin oxygen saturation measurements confirmed these findings ( p & lt;.02 ) . -
pubmed
Instead , the mutant assembled efficiently in buffers containing
CaCl2 & gt ; or = 6 mM forming filaments that were 10 times longer than those formed by wt
NF - L , although their diameter was significantly smaller ( 6 - 7 nm ) . -
pubmed
Bacteria were exposed to a
nitrogen laser ( average power 2.0 mW ) in vitro at power density of 70 + /- 0.7 W / m2 for 0 - 30 min , and the cell viability was determined by
luciferase reporter phage (
LRP ) assay . -
pubmed
Electron paramagnetic resonance (
EPR ) studies using
5 - doxyl strearic acid (
5 - DS ) as a probe suggest alterations in lipid regions of the cell wall . -
pubmed
PTH and interactions with
bisphosphonates . -
pubmed
The failure of the ' washout ' period to restore the normal response to
PTH is suggestive of a physico - chemical interaction on the level of the matrix embedded
bisphosphonate with the overlaying bone lining cells , rather than of direct effects of the drug on osteoblasts or their precursor cells . -
pubmed
Clinical investigators studying anabolic drugs such as
PTH should be aware of potential long - term interactions of
bisphosphonates when assessing the outcome of their experiments . -
pubmed
However , the beneficial effect of
bisphosphonates like
alendronate on
PTH - induced
bone remodeling , as well as its potent action in the protection of
bone loss after cessation of anabolic therapy might outweigh the worries about a small delay in the bone response to
parathyroid hormone . -
pubmed
Clinical trial data and real - world experience have shown the
nonnucleoside reverse transcriptase inhibitors ( NNRTIs ) to be an important class of agents for the treatment of
HIV . -
pubmed
The preparation and biological evaluation of
5 - substituted-6 - hydroxy-2 -( anilino ) pyrimidinones as a new class of
DNA polymerase IIIC inhibitors , required for the replication of chromosomal DNA in Gram - positive bacteria , are described . -
pubmed
Ca6Cu2Sn7 crystallizes in the monoclinic space group
C2 / m ( No . -
pubmed
In cell - free systems ,
p53R2 did not oxidize a reactive
oxygen species ( ROS ) indicator
carboxy - H2DCFDA , but another class I RR small subunit ,
hRRM2 , did . -
pubmed
Further studies showed that purified recombinant
p53R2 protein has
catalase activity , which breaks down
H2O2 . -
pubmed
The fluorescence intensity in mutants oxidizing
carboxy - H2DCFDA , in order from highest to lowest , was Y331F & gt ; Y285F & gt ; Y49F & gt ; Y241H & gt ; wild - type
p53R2 . -
pubmed
Of interest , the ability to oxidize
carboxy - H2DCFDA indicated by fluorescence intensity was negatively correlated with
RR activity from wild - type
p53R2 , mutants Y331F , Y285F , and Y49F. -
pubmed
A simple , sensitive and specific LC -
MS /
MS method for the simultaneous determination of
sulforaphane (
SFN ) and its major metabolites , the
glutathione (
SFN - GSH ) and
N - acetyl cysteine conjugates (
SFN - NAC ) from biological matrices was developed and validated . -
pubmed
Indo - mo investigation of gas - phase acidities of
carbamates and
thiocarbamates . -
pubmed
He received conventional therapy (
steroids ,
mycophenolate , anti -
IL-2 monoclonal antibodies ,
anti - thymocyte globulin ) without success . -
pubmed
Ischemia promotes deacetylation of core
histone proteins H3 and
H4 and dimethylation of
histone H3 at
lysine-9 (
H3 - K9 ) over the
MOR-1 promoter , an signature of epigenetic gene silencing . -
pubmed
A series of
2 - alkylthio-1 -[ 4 -( 1 - benzyl-2 - ethyl-4 - nitro-1H - - imidazol-5 - yl )- piperazin-1 - yl ] ethanones ( 3 - 9 ) and
alkyl -[ 4 -( 1 - benzyl-2 - ethyl-4 - nitro-1H - imidazol-5 - yl )- piperazin-1 - yl ) ketones ( 11 - 20 ) as well as the
indole analogue 22 were synthesized from
4 - nitro-5 - piperazinyl imidazole derivative 1 , with the aim to develop newly non -
nucleoside reverse transcriptase inhibitors ( NNRTIs ) . -
pubmed
A novel molecularly imprinted polymer (
MIP ) selective for
tryptophan (
Trp ) was described where polymerization was performed in aqueous media . -
pubmed
The results of adsorption experiments indicated that the selectivity of
MIP , which was synthesized with bonded
beta - CD and
AA [ MIP ( 1 ) ] , was superior to those obtained with
AA [ MIP ( 2 ) ] or bonded beta -
CD [ MIP(3 ) ] . -
pubmed
Synthesis of
2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.2 ] nonane ,
2 -( pyridin-3 - yl )- 1 - azabicyclo [ 2.2.2 ] octane , and
2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.1 ] octane , a class of potent
nicotinic acetylcholine receptor - ligands . -
pubmed
In an attempt to generate
nicotinic acetylcholine receptor (
nAChR ) ligands selective for the
alpha4beta2 and alpha7 subtype receptors we designed and synthesized constrained versions of
anabasine , a naturally occurring
nAChR ligand . -
pubmed
All compounds synthesized exhibited high affinity for the
alpha4beta2 nAChR subtype ( K i & lt ; or = 0.5 - 15 nM ) , a subset bound with high affinity for the
alpha7 receptor subtype ( K i & lt ; or = 110 nM ) , selectivity over the
alpha3beta4 ( ganglion ) receptor subtype was seen within the
2 -( pyridin-3 - yl )- 1 - azabicyclo [ 2.2.2 ] octane series and for the muscle ( alpha1betagammadelta ) subtype in the
2 -( pyridin-3 - yl )- 1 - azabicyclo [ 3.2.2 ] nonane series . -
pubmed
An
ATR - FTIR experiment showed features at 1632 and 1602 cm(-1 ) that are attributed to
NO ( 2 ) complexed to
NO ( 3 )(- ) and
HNO ( 3 ) , respectively . -
pubmed
Community analysis of betaproteobacterial
ammonia - oxidizing bacteria using the
amoCAB operon . -
pubmed
The genes and intergenic regions of the
amoCAB operon were analyzed to establish their potential as molecular markers for analyzing
ammonia - oxidizing betaproteobacterial ( beta - AOB ) communities . -
pubmed
NO - mediated S - nitrosation of
cysteine residues has been recognized as a fundamental
post - translational modification . -
pubmed
Moreover , the
nitrosocysteine residue for each
protein (
HSPA9/368 ,
beta - actin / 16 ,
TMP3 / 170 ,
vimentin / 328 ) was also determined , and the relative ratio of S - nitrosation / non - S - nitrosation for
Cys328 of
vimentin was estimated using MASIC software . -
pubmed
By the combination of the
biotin switch method with
2 - DE and Western blot analysis , S - nitrosoproteins can be screened and characterized by
MS , providing a basis for further study of the physiological significance of each
S - nitrosoproteins . -
pubmed
Instead , we show that
nitrite enhances the
ATP release from RBCs , which is known to produce vasodilation by several different methods including the interaction with
purinergic receptors on the endothelium that stimulate the synthesis of
NO by
endothelial NO synthase . -
pubmed
The observed decrease in blood pressure was not observed if
endothelial NO synthase was inhibited by
N ( omega )- nitro - L - arginine methyl ester (
L - NAME ) or when any released
ATP was degraded by
apyrase . -
pubmed
The
nitrite - enhanced
ATP release was shown to involve an increased binding of
nitrite - modified
hemoglobin to the RBC membrane that displaces
glycolytic enzymes from the membrane , resulting in the formation of a pool of
ATP that is released from the RBC . -
pubmed
The
phosphatase KdsC cleaves
3 - deoxy - D - manno - octulosonate 8 - phosphate to generate a molecule of inorganic
phosphate and
Kdo . -
pubmed
KdsC belongs to the broad
haloacid dehalogenase superfamily . -
pubmed
It is therefore not clear why
KdsC , which lacks a cap domain , is catalytically efficient and highly specific to
3 - deoxy - D - manno - octulosonate 8 - phosphate . -
pubmed
When treated in vivo with
cyclophosphamide ,
tumors coexpressing
Mcl-1 and
Myc transgenes were significantly more resistant than conventional Emu - Myc
lymphomas . -
pubmed
By using the tripodal ligand
ntb ( tris ( benzimidazole-2 - ylmethyl ) amine ) and
lanthanide nitrate , three isomorphous series of coordination frameworks of the general formula
[ Ln ( ntb )( NO ( 3 ))( 3 ) ] solvents ( series 1 : monoclinic
C2 / c , Ln =
Gd ( 3 + ) and
Yb ( 3 + ) ; series 2 : hexagonal P3(1)/c ,
Ln = Nd ( 3 + ) ,
Eu ( 3 + ) ,
Gd ( 3 + ) , and
Er ( 3 + ) ; series 3 , cubic
Pa3 ,
Ln =
Gd ( 3 + ) and
Er ( 3 + ) ; solvent =
H ( 2 ) O or
CH ( 3 ) OH ) have been assembled and characterized with IR , elemental analyses , and single crystal and powder X - ray diffraction methods . -
pubmed
These findings support clinical development of
daratumumab for the treatment of
CD38 - positive
MM tumors . -
pubmed
p27 gene encodes a lipoprotein that binds triacylated glycolipids and modulates the host immune responses by inhibiting the
MHC - II Ag processing . -
pubmed
pneumoniae
HMGR with K(i ) values in the range of 6.45 - 20.49 muM than the statin drug
lovastatin ( K(i)=116.25 muM ) , a classical inhibitor of
class I HMGR . -
pubmed
In some cases ,
I prostanoid ( IP ) receptor and
E prostanoid receptor antagonists were added before the
PGI2 analog treatment . -
pubmed
T - cell polarization function was investigated by measuring
interferon gamma ,
interleukin 13 (
IL-13 ) , and
IL-17A production by T cells cocultured with
iloprost - treated
MDDCs . -
pubmed
RESULTS :
Iloprost and
treprostinil suppressed LPS - induced
TNF - alpha expression in
MDDCs . -
pubmed
This effect could be reversed by an
IP receptor antagonist ,
CAY10449 , but not by
E prostanoid receptor antagonists . -
pubmed
Iloprost suppressed the LPS - induced expression of
costimulatory molecules , including
CD80 ,
CD86 ,
CD40 , and
HLA - DR . -
pubmed
Iloprost - treated MDDCs increased
IL-17A production by T cells . -
pubmed
Automated sequence analysis of exons and intron - exon boundaries did not reveal any genetic variation in the
NOTCH3 gene , and
electron microscopy excluded the presence of
GOM . -
pubmed
Hexose transporter GLUT1 harbors several distinct regulatory binding sites for
flavones and
tyrphostins . -
pubmed
The facilitative
hexose transporter
GLUT1 activity is blocked by
tyrosine kinase inhibitors that include
natural products such as
flavones and
isoflavones and synthetic compounds such as
tyrphostins ,
molecules that are structurally unrelated to the transported substrates [ Vera , et al . -
pubmed
Here we analyzed the interaction of
GLUT1 with
quercetin ( a
flavone ) ,
genistein ( an
isoflavone ) , and
tyrphostin A47 and B46 to evaluate if they share one common or have several binding sites on the protein . -
pubmed
Genistein protected
GLUT1 against
iodide - elicited fluorescence quenching and also decreased the affinity of
d - glucose for its external binding site , while
quercetin and
tyrphostins B46 and
A47 promoted fluorescence quenching and did not affect the external
d - glucose binding site . -
pubmed
These findings are explained by a carrier that presents at least three binding sites for
tyrosine kinase inhibitors , in which ( i )
genistein interacts with the transporter in a conformation that binds
glucose on the external surface ( outward - facing conformation ) , in a site which overlaps with the external binding site for
d - glucose , ( ii )
quercetin and
tyrphostin B46 interact with the
GLUT1 conformation which binds
glucose by the internal side of the membrane ( inward - facing conformation ) , but to a site accessible from the external surface of the protein , and ( iii ) the binding site for
tyrphostin A47 is accessible from the inner surface of
GLUT1 by binding to the inward - facing conformation of the transporter . -
pubmed
The concordance for subject classification according to the National
Cholesterol Education Program ( NCEP ) guidelines was significant ( P & lt ; 0.001 ) , with substantial agreement for
TC ( K(w ) = 0.67 ) , and moderate agreement for
TG ( K(w ) = 0.50 ) . -
pubmed
Mutation of plasma membrane
Ca2 + ATPase isoform 3 in a family with
X - linked congenital cerebellar ataxia impairs
Ca2 + homeostasis . -
pubmed
Membrane transporters , among them the
calmodulin ( CaM )- activated plasma membrane Ca ( 2 + ) ATPases (
PMCAs ) that extrude
Ca ( 2 + ) from the cell , play a key role in neuronal
Ca ( 2 + ) homeostasis . -
pubmed
The mutation reduced the ability of the
PMCA3 pump to control the cellular homeostasis of
Ca ( 2 + ) . -
pubmed
In addition ,
eugenol stimulated
PAF - acetylhydrolase activity suggesting that inhibition of PAF could be due to its inactivation to
lyso - PAF . -
pubmed
Retinaldehyde dehydrogenase 3 (
RALDH3 ) is the key enzyme required to generate
retinoic acid within the olfactory epithelium . -
pubmed
PCR primers were developed and applied to target the
sulfur oxidation
soxB gene of Epsilonproteobacteria . -
pubmed
Although Sulfurovum - like
soxB genes were detected in all fluid samples , the RNA profiles were nearly identical among the vents and suggest that Sulfurimonas - like species are the primary Epsilonproteobacteria responsible for actively oxidizing
sulfur via the
Sox pathway at each vent . -
pubmed
Community patterns of subseafloor Epsilonproteobacteria
16S rRNA genes were best matched to
methane concentrations in vent fluids , as well as individual vent locations , indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations . -
pubmed
Formation of
cell surface FHC dimers is critically dependent on an unpaired reactive
cysteine 67 in the alpha1 helix of the
class I heavy chain . -
pubmed
Sorafenib is an orally administered active
multikinase inhibitor for metastatic
renal cell carcinoma that is now considered a standard agent . -
pubmed
OBJECTIVE : The aim of the study was to compare annual bone mineral density (
BMD ) changes among new users of
selective serotonin reuptake inhibitors ( SSRIs ) , new users of tricyclic antidepressants (
TCAs ) , and nonusers of antidepressant medications . -
pubmed
RESULTS :
BMD was compared among 311 new users of SSRIs , 71 new users of
TCAs , and 1590 nonusers . -
pubmed
After adjustment for potential confounders , including age , race ,
body mass index , menopausal status , and hormone therapy use , mean lumbar spine
BMD decreased on average 0.68 % per year in nonusers , 0.63 % per year in SSRI users ( P = .37 for comparison to nonusers ) , and 0.40 % per year in
TCA users ( P = .16 for comparison to nonusers ) . -
pubmed
METHODS : To avoid ambiguities owing to the heterogeneous nature , we experimented with hESC - VCMs , purified on the basis of
zeocin resistance and signature ventricular action potential after LV -
MLC2v - tdTomato - T2A - Zeo transduction . -
pubmed
RESULTS :
Ca ( 2 + ) sparks that were sensitive to inhibitors of
sarco / endoplasmic reticulum Ca ( 2 +)- ATPase (
thapsigargin and
cyclopiazonic acid ) and
ryanodine receptor (
RyR ;
ryanodine ,
tetracaine ) but not
inositol trisphosphate receptors (
xestospongin C and
2 - aminoethyl diphenylborinate ) could be recorded . -
pubmed
In a permeabilization model , we further showed that
RyRs could be sensitized by
Ca ( 2 + ) . -
pubmed
Furthermore ,
RyR - mediated
Ca ( 2 + ) release sensitized nearby
RyRs , leading to compound
Ca ( 2 + ) sparks . -
pubmed
By contrast , removal of external
Na (+ ) or the addition of the
Na (+)- Ca ( 2 + ) exchanger inhibitor (
KB - R7943 and
SN-6 ) had no effect , suggesting that the
Na (+)- Ca ( 2 + ) exchanger is not involved in triggering sparks . -
pubmed
In the above backdrop , we report the synthesis of a novel , stable , cross - linked
polyester with
salicylic acid (
SA ) incorporated in the polymeric backbone and propose a simple synthesis route by melt condensation . -
pubmed
The combination of NMR and FT - IR spectral techniques established the
ester linkages in the as - synthesized
SA - based
polyester . -
pubmed
The pH - dependent degradation rate and the rate of release of
salicylic acid from the as - synthesized
SA - based polymer were studied at physiological conditions in vitro . -
pubmed
Our results strongly suggest that
SA - based
polyester supports cell proliferation for 3 days in culture and do not cause cell
death ( & lt;7 % ) , as quantified by
propidium iodide (
PI ) stained cells . -
pubmed
Novel immobilization of a
quaternary ammonium moiety on
keratin fibers for medical applications . -
pubmed
After each resistance exercise session , subjects were randomly divided into two groups with respect to intake of protein ( 0.3 g / kg
body weight ) and
carbohydrate ( 0.8 g / kg
body weight ) immediately after (
P0 ) or 6 h ( P6 ) after the session . -
pubmed
We assessed whole - body protein metabolism by measuring
nitrogen balance at
P0 and P6 on the last 3 days of exercise training . -
pubmed
RESULTS : The
nitrogen balance was significantly lower in the trained men than in the untrained men at both
P0 ( P & lt;0.05 ) and P6 ( P & lt;0.01 ) . -
pubmed
The
nitrogen balance in trained men was significantly higher at
P0 than at P6 ( P & lt;0.01 ) , whereas that in the untrained men was not significantly different between the two periods . -
pubmed
Specific inhibitors of the
ERK or
NF - kappaB pathway (
PD98059 or
Bay11 - 7082 ) suppressed
TNF - alpha - induced
MMP-2 expression in
leiomyoma SMCs . -
pubmed
The
wound - healing assay revealed that
TNF - alpha promoted the migration of cultured
leiomyoma SMCs ( P = 0.036 ) ; however ,
PD98059 compromised the cell migration triggered by
TNF - alpha . -
pubmed
WIDER IMPLICATIONS OF THE FINDINGS : Considering the positive effect of
TNF - alpha on
leiomyoma SMC migration , strategies targeting
TNF - alpha , in parallel with the production of more specific
inhibitors of
MMPs , may provide alternative therapeutic approaches for the treatment of
leiomyoma . -
pubmed
In contrast , the
Cyanide - Insensitive
Oxidase type may have differentiated into recognizable subtypes after another gene cluster duplication . -
pubmed
[ Application of diagnosticum based on functionalized
carbon nanoparticles for monitoring of affine
immunoglobulin purification ] . -
pubmed
IgG detection sensitivity using the
carbon - G - protein diagnosticum was 80 ng / mL. -
pubmed
In the nodules , bacteria differentiate into bacteroids with the ability to fix atmospheric
N2 via
nitrogenase activity . -
pubmed
As
nitrogenase is strongly inhibited by
oxygen ,
N2 fixation is made possible by the microaerophilic conditions prevailing in the nodules . -
pubmed
Chronic
ethanol consumption is known to downregulate expression of the major
glutamate transporter 1 (
GLT-1 ) , which increases extracellular
glutamate concentrations in subregions of the mesocorticolimbic reward pathway . -
pubmed
While
beta - lactam antibiotics were initially identified as potent upregulators of
GLT-1 expression , only
ceftriaxone has been extensively studied in various
drug addiction models . -
pubmed
In addition , only
cefoperazone significantly inhibited hepatic
aldehyde dehydrogenase-2 enzyme activity . -
pubmed
Chemicals that induce
cytochrome P450 ( P450 ) 1A2 , a major
enzyme involved in the bioactivation of
HAAs , also form in cooked meat . -
pubmed
Therefore , well - done cooked meat may pose an increase in
cancer risk because it contains both inducers of
P450 1A2 and procarcinogenic
HAAs . -
pubmed
The mean
P450 1A2 activity , assessed by
caffeine metabolic phenotyping , ranged from
6.3 to 7.1 before the feeding study commenced and from 9.6 to 10.4 during the meat feeding period : the difference in means was significant ( P & lt ; 0.001 ) . -
pubmed
Unaltered
PhIP ,
MeIQx , and their
P450 1A2 metabolites ,
N ( 2 )-( beta-1 - glucosiduronyl )- 2 -( hydroxyamino )- 1 - methyl-6 - phenylimidazo [ 4,5 - b ] pyridine (
HON - PhIP - N ( 2 )- Gl ) ;
N3 -( beta-1 - glucosiduronyl )- 2 -( hydroxyamino )- 1 - methyl-6 - phenylimidazo [ 4,5 - b ] pyridine (
HON - PhIP - N3 - Gl ) ;
2 - amino-3 - methylimidazo -[ 4,5 - f ] quinoxaline-8 - carboxylic acid (
IQx-8 - COOH ) ; and
2 - amino-8 -( hydroxymethyl )- 3 - methylimidazo [ 4,5 - f ] quinoxaline (
8 - CH2OH - IQx ) were measured in urine during days 2 , 14 , and 28 of the meat diet . -
pubmed
However , there was no statistically significant correlation between the
caffeine P450 1A2 phenotype and any urinary
HAA biomarker . -
pubmed
The
caffeine metabolic phenotype for
P450 1A2 was a poor predictor of oxidative urinary metabolites of
PhIP and
MeIQx and may not be a reliable measure to assess the role of
HAAs in
cancer risk . -
pubmed
The accumulation of
PrPSc in cells persistently infected with the prion
strain Fukuoka-1 ( FK ) , derived from a patient with
Gerstmann - Straussler - Scheinker syndrome , was significantly increased in cultures treated with the macroautophagy inhibitor
3 - methyladenine (
3MA ) but substantially reduced in those treated with the macroautophagy inducer
rapamycin . -
pubmed
By contrast , neither
rapamycin nor
3MA had any apparently effect on PrPSc from either the 22L or the Chandler
strain , indicating that the degradation of
PrPSc in host cells might be strain - dependent . -
pubmed
At 3d ,
DCE increased
infarct size ( CMR : 18.8 % vs. 14.5 % , p = 0.04 ; serum
troponin - I : 13.3 vs. 6.9 ng / uL , p & lt ; 0.05 ) and
MMP-2 activity levels ( 0.81 vs. 0.49 , p = 0.002 ) , with reduced activation of
Akt ( 0.06 versus 0.26 , p = 0.02 ) . -
pubmed
The significance of the later remodelling changes ( ventricular thinning and transmurality ) following
DCE , possibly due to changes in
MMP-2 activity and
Akt activation , merits further study . -
pubmed
Alanine and
aspartate aminotransferases (
ALT ,
AST ) and
creatine kinase (
CK ) activities were measured spectrophotometrically at 340 nm after acrosome reaction with P4 . -
pubmed
Acrosome reaction induction by P4 required different
creatine kinase activity with the same
oxygen consumption and
transaminases level to maintain oxidative metabolism and redox state through reducing equivalents transfer between cytosolic and mitochondrial compartment . -
pubmed
These data show that
ATP binds
Dbp5 weakly in rapid equilibrium with a binding affinity ( KT~4 mM ) comparable to the KM for steady - state cycling , while
ADP binds an order of magnitude more tightly ( KD~0.4 mM ) . -
pubmed
The growing industrial application of
imidazolium - type ionic liquids (
ITILs ) is likely to result in their release to the environment . -
pubmed
We studied the effects of
beta - adrenoceptor antagonists
propranolol and
nadolol and
L - type Ca ( 2 +)- channel blocker
verapamil on
cardiac reperfusion injury developed after 45 - min
coronary occlusion . -
pubmed
Suppression of
Nfib expression in cell lines derived from the
doxycycline - inducible
Rb /
p53 /
TET -
Nfib model led to increased apoptosis and suppression of proliferation . -
pubmed
Photoactivatable -
Ribonucleoside - Enhanced Crosslinking and Immunoprecipitation (
PAR -
CLIP ) in combination with next - generation sequencing is a powerful method for identifying endogenous targets of RNA - binding proteins ( RBPs ) . -
pubmed
Positive matrix factorization (
PMF ) with the multi - linear engine ( ME-2 ) solver identified six organic aerosol ( OA ) factors , including
hydrocarbon - like OA (
HOA ) , coal combustion OA ( CCOA ) , cooking - related OA ( COA ) , biomass burning OA ( BBOA ) and two oxygenated OA ( OOA1 and OOA2 ) , which accounted for 8.5 % , 20.2 % , 18.6 % , 12.4 % , 17.8 % and 22.5 % of the total organics mass on average , respectively . -
pubmed
Drug combination approach to overcome resistance to
EGFR tyrosine kinase inhibitors in
lung cancer . -
pubmed
Results showed that acupuncture was an effective treatment method in inhibiting inflammatory conditions , serum
IgE levels , and expression of proinflammatory
cytokine Th2 (
IL-4 ,
IL-6 ) , and Th2 ( IL-1beta , TNF - alpha ) mRNA compared with
DNCB treatment . -
pubmed
BACKGROUND :
Autosomal recessive distal renal tubular acidosis (
dRTA ) is a rare
hereditary disease caused by pathogenic variants in the
ATP6V0A4 gene or
ATP6V1B1 gene , and characterized by hyperchloremic
metabolic acidosis with normal
anion gap ,
hypokalemia ,
hypercalciuria ,
hypocitraturia and
nephrocalcinosis . -
pubmed
Uric acid ,
albumin ,
vitamins are some of non enzymatic molecular antioxidants . -
pubmed
Parabens are known to be weak endocrine disruptors because they interfere with the function of endogenous hormones through binding to
estrogen receptors . -
pubmed
Parabens were separated using a Waters BEH
C18 column ( 2.1 mm x 100 mm , 1.7 mum ) and a mobile phase consisting of 10 mM
ammonium acetate in water and
acetonitrile with a gradient program at a flow rate of 300 muL / min . -
pubmed
Efficacy and safety of bispecific
T - cell engager ( BiTE ) antibody
blinatumomab for the treatment of relapsed / refractory
acute lymphoblastic leukemia and
non - Hodgkin ' s lymphoma : a systemic review and meta - analysis . -
pubmed
Measurement of
vitamin D ,
iron ,
zinc ,
thyroid hormones , and
prolactin are optional but recommended . -
pubmed
( vi ) Treatment of
FPHL should start with
minoxidil ( 5 % ) , adding
5alpha - reductase inhibitors or antiandrogens when there is severe
hair loss or
hyperandrogenism . -
pubmed
Furthermore , IONP -
PEG activated in vitro , the complement system and induced a substantial increment of
C3a and
C4a anaphylatoxins while IONP - bare and IONP -
PVP did not . -
pubmed
In vivo IONP -
PEG induced an increment in
complement activation markers (
C5a and
C5b-9 ) , and proinflammatory
cytokines (
IL-1beta ,
IL-6 ,
TNF - alpha ) . -
pubmed
In fungi , green light is absorbed by
rhodopsins ,
opsin proteins carrying a
retinal molecule as chromophore . -
pubmed
Also ,
UmOps1 showed a pronounced , concentration - dependent enhancement of pump current caused by weak organic acids ( WOAs ) , especially by
acetic acid and
indole-3 - acetic acid (
IAA ) . -
pubmed
MTAP Loss Promotes Stemness in
Glioblastoma and Confers Unique Susceptibility to
Purine Starvation . -
pubmed
As a combined consequence of
purine production deficiency in
MTAP - null
GBM and the critical dependence of GSCs on
purines , the enriched subset of
CD133 + cells in
MTAP - null
GBM can be effectively depleted by inhibition of de novo
purine synthesis . -
pubmed
TECA , AST and the TECA+AST reatments inhibited the expression of
iNOS and
COX-2 ;
NF - kappaB activity ; and the release of
TNF - alpha ,
IL-6 and
IgE. -
pubmed
CONCLUSIONS : Our results demonstrate that the combination of
TECA and
AST could be a promising therapeutic agent for
AD by inhibiting
NF - kappaB signaling . -
pubmed
By chemical methods and
GC ,
GC -
MS , 1D and 2D NMR spectroscopy was established that the arabinogalactan consists of
d - galactopyranose residues linked by beta-1,6 - glycosidic bonds as a main chain , and the side chain was represented by
alpha - arabinose ,
beta - galactose and
4 - O - methylglucuronic acid . -
pubmed
LncRNA -
MALAT1 mediates
cisplatin resistance via miR-101 - 3p /
VEGF - C pathway in
bladder cancer . -
pubmed
However , the roles and underlying molecular mechanisms of
MALAT1 in
cisplatin resistance of the
BC cells remain largely unclear . -
pubmed
Secondly , we found that suppression of
MALAT1 enhanced the chemotherapeutic drug sensitivity and inhibited the
cisplatin resistance of the
BC cells . -
pubmed
Thirdly , we showed that
MALAT1 affected the
cisplatin resistance of the
BC cells via regulating the miR-101 - 3p /
VEGF - C pathway . -
pubmed
In summary , this study demonstrates that
MALAT1 , miR-101 - 3p and
VEGF - C form a regulatory axis to affect the chemo - resistance of
BC cells to
CDDP , and provides novel potential targets for treatment of
BC . -
pubmed
Furthermore , we found that
coixol inhibits
mitogen - activated protein kinases (
MAPKs ) ,
nuclear transcription factor kappa B (
NF - kappaB ) pathways , and
NOD - like receptor protein ( NLRP ) 3 inflammasome activation . -
pubmed
Estrogen receptors (
ERs ) are
steroid hormone receptors with important functions in neurogenesis and synaptic plasticity . -
pubmed
Carbogen breathing significantly increased VF in
BG and WM , and rCNR in thalamus ,
BG , and WM compared to air breathing . -
pubmed
The COP - QP -
TC was successfully applied for the SPME of some
PAHs and their derivatives from environmental water samples with good method recoveries ranging from 85.8 % to 114.4 % . -
pubmed
Sre1 directly regulates genes related to
ergosterol biosynthesis as well as genes related to the
mevalonate pathway , which synthesizes the building blocks of
isoprenoids , including carotenoids . -
pubmed
dendrorhous is regulated by
Sre1 through the regulation of the
mevalonate pathway and the regulation of the
crtE and
crtR genes . -
pubmed
As the
crtR gene encodes a
cytochrome P450 reductase ,
Sre1 regulates pathways that include
cytochrome P450 enzymes , such as the biosynthesis of carotenoids and
sterols . -
pubmed
OBJECTIVE : To evaluate the role of PET /
CT to visualize the immune - related adverse events ( irAEs ) following
pembrolizumab . -
pubmed
INTERVENTION : PET /
CT before and after neoadjuvant
pembrolizumab , before radical cystectomy . -
pubmed
OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS : We analyzed the occurrence of irAEs , evaluated according to the Common Terminology Criteria for Adverse Events ( CTCAE ) version 5.0 , against the development of inflammatory
FDG uptake described at PET /
CT ( irAEs + PET /
CT ) . -
pubmed
Logistic regression analyses evaluated the association between irAEs + PET /
CT and the pathological response to
pembrolizumab . -
pubmed
RESULTS : Compared with baseline ,
EPA and
DHA supplementation differently modulated LPS - stimulated monocyte
cytokine expression . -
pubmed
EPA lowered
TNFA ( p < 0.001 ) whereas
DHA reduced
TNFA ( p < 0.001 ) ,
IL6 ( p < 0.02 ) ,
MCP1 ( p < 0.03 ) , and
IL10 ( p < 0.01 ) . -
pubmed
DHA lowered
IL10 expression relative to
EPA ( p = 0.03 ) . -
pubmed
Relative to baseline ,
EPA , but not
DHA , decreased the ratios of
TNFA /
IL10 and
MCP1 /
IL10 ( both p < 0.01 ) . -
pubmed
Different groups of
PUFA derivatives mediated the differential effects of
EPA and
DHA on monocyte
cytokine expression . -
pubmed
CONCLUSIONS :
EPA and
DHA had distinct effects on monocyte inflammatory response with a broader effect of
DHA in attenuating pro - inflammatory
cytokines . -
pubmed
In vivo recordings of Schaffer collateral stimulation - induced synaptic responses during
IH in the
CA1 region of the hippocampus revealed pathological late phase hypoxic long term potentiation ( hLTP ) ( 154 % ) that lasted more than four hours and could be reversed by depotentiation with low frequency stimulation ( LFS ) , or abolished by
NMDA and
PKA inhibitors (
MK-801 and
CMIQ ) . -
pubmed
Mechanism of Two Typical Binders
BR and F2604 on Thermal Decomposition of
HMX . -
pubmed
DSC -
TG - FTIR -
MS coupling technology was used to study the mechanism of two typical binders , that is ,
BR and F2604 , on the thermal decomposition behavior of the
HMX crystal . -
pubmed
Especially in the case of
HMX /
BR particles , the decomposition temperature is the lowest , but the activation energy is the highest . -
pubmed
Based on the results of DSC -
TG - FTIR -
MS , it is found that the rapid mechanism of binder and active intermediate products inhibits the reaction of relatively inert intermediate products and prolongs the continuous generation time of gas products in the composite particles , which delays the decomposition of
HMX to a certain extent . -
pubmed
Neurotransmitters involved in
ACTH secretion :
catecholamines . -
pubmed
The presence of these
enzyme activites would allow
D - fructose to be degraded by the Embden - Meyerhof pathway and/or the Entner - Doudoroff pathway . -
pubmed
Glucose 15 mM did not further enhance the
cAMP response , although this concentration increased
insulin secretion more than two - fold over values observed at
glucose 10 mM. -
pubmed
Thus , the
glucose dose - response relations for
cAMP levels and
insulin secretion appear to be different , indicating that factors other than
cAMP alone determine the secretory response to
glucose . -
pubmed
Fasting for 24 and 72 h progressively inhibited
glucose - induced
insulin secretion . -
pubmed
Fasting for 24 h shifted the
glucose dose - response curves for
cAMP and
insulin secretion to the right , but the maximum responses at
glucose 37.5 mM were not significantly inhibited . -
pubmed
The results support the view that inhibition of the
adenylate cyclase -
cAMP system is a major determinant factor in fasting - induced impairment of
insulin secretion during the initial 30 min of
glucose stimulation . -
pubmed
NADPH - cytochrome c reductase (
EC 1.6.2.3 ) was solubilized and purified 4 - 6 fold by three methods with use of sonication and detergent digestion followed by either
DEAE - cellulose chromatography or
ammonium sulfate fractionation . -
pubmed
Reductase fractions prepared by
sodium cholate digestion of microsomes were highly active in supporting the hydroxylation activity in the reconstituted systems , whereas those prepared with
sodium deoxycholate were not . -
pubmed
These filaments are distinct from the
actin microfilament bundles visualized by an antibody against
actin , and they are not affected by brief treatment with
cytochalasin B. -
pubmed
In response to the drugs
colcemid and
vinblastine , the filaments reacting with this preimmune serum condense to form a compact perinuclear
coil of fibers , a distribution and behavior in agreement with that previously described for the 10 nm or intermediate filaments studied by electron microscopy . -
pubmed
At the same time , certain doses of each of the
platinum compounds induced selective recovery of the preantigen reactive cells ( P - PFC ) and myelocytic progenitor cells (
CFC ) in the bone marrow with a pattern that was unique for each compound . -
pubmed
Effects of
melanocyte - stimulating hormone (
MSH ) and
melatonin on passive avoidance and on an emotional response . -
pubmed
The present experiment investigated the opposite effects of synthetic
alpha - MSH and
Melatonin on acquisition and extinction of a passive avoidance response (
PAR ) and on emotionality , as indexed by defecation , in the
PA box . -
pubmed
It was found that intraperitoneal (
IP ) administration of
alpha - MSH delayed extinction and increased defecation responses whereas
IP administration of
Melatonin facilitated extinction of the
PAR and decreased defecation . -
pubmed
The present experiment confirmed
MSH -
Melatonin opposition on memory and on the defecation response . -
pubmed
Since the
Dw4 , Dw14 , Dw15 , and DR1 molecules have similar
antigen - binding sites and since combinations of these alleles particularly predispose to severe
RA , we suggest that synergistic mechanisms are involved . -
pubmed
Nucleotide sequence of
rabbit elongation factor 1 alpha cDNA . -
pubmed
Previous studies have reported elevated
interleukin 1 (
IL-1 ) ,
prostaglandin E2 (
PGE2 ) , and
collagenase in this tissue . -
pubmed
2 ) The greatest skin response with regard to
histamine was found for
DFA ( 52 % ) ,
DPT ( 44 % ) ,
HD ( 22 % ) ,
GD ( 10 % ) ,
AS ( 4 % ) and
GF ( 2%). -
pubmed
It is possible that longer retention of
captopril in the plasma , evidence by larger AUC , may reflect less
captopril available to modulate
renin activity in the kidney . -
pubmed
It was found that
heparin concentration of 3 to 500 U per 1 kg
collagen causes a hyperadhesive effect on blood platelets and facilitates the formation of the primary thrombocytic
thrombus . -
pubmed
Clindamycin , which enters phagocytes by the cell membrane
adenosine (
nucleoside ) transport system , had only a modest effect on
FMLP - mediated
superoxide production but inhibited the microbial particle - induced response by approximately 50 % . -
pubmed
Dideoxy sequencing of RNA using
reverse transcriptase . -
pubmed
Serology , absorption experiments and family studies indicate that
SNA antigen is a
Bw6 associated subtype of
B5 antigen closely related to but clearly distinct from each of the
B5 CREG antigens . -
pubmed
Blockade of
morphine - induced increases in brain
tryptophan hydroxylase activity by systemic pretreatment with
CCK-8 : no reversal by vagotomy . -
pubmed
We have investigated the possibility that peripherally administered
CCK-8 can also suppress a neurochemical response to opiates , namely the increase in brain
tryptophan hydroxylase (
TrpH ) activity and tissue
5 - hydroxyindole acetic acid (
5 - HIAA ) which results from
morphine administration . -
pubmed
roughly doubled brain
TrpH activity and tissue
5 - HIAA 40 min after injection , pretreatment with
CCK-8 ( 5 micrograms / kg i.p . -
pubmed
Doses of
CCK-8 as low as 1 microgram / kg were effective in blunting the
morphine - induced increase in cortical
TrpH activity , but the desulfated form was ineffective at doses of 5 and 100 micrograms / kg . -
pubmed
Alpha - difluoromethylornithine (
DFMO ) , an irreversible inhibitor of
ornithine decarboxylase , has been demonstrated to be an effective agent against a variety of parasitic protozoa but not against Leishmania spp . -
pubmed
Hydralazine - induced
systemic lupus erythematosus : influence of
HLA - DR and sex on susceptibility . -
pubmed
26 patients with
systemic lupus erythematosus (
SLE ) induced by treatment with the antihypertensive drug
hydralazine were investigated to determine if predisposition to the toxic effect was associated with an
HLA - DR antigen . -
pubmed
The frequency of
HLA - DR4 ( 73 % ) was significantly higher in the group with
hydralazine - induced
SLE than in the other groups ( respectively 33 % , 25 % , and 25 % ) . -
pubmed
If the slow acetylators treated with
hydralazine were analysed as one group , it was observed that all women with
DR4 developed
hydralazine - induced
SLE ; the only men to do so were those with DR2 who were receiving 200 mg
hydralazine per day . -
pubmed
These observations have led us to suggest guide lines for
hydralazine therapy and point to a striking association between an
HLA - DR antigen and an adverse reaction to a therapeutic agent . -
pubmed
It was also noted that the distribution of
DR antigens in the
hydralazine -
SLE patients was significantly different from that in the group with
idiopathic SLE . -
pubmed
Plasma extracted using leached
silica glass (
Vycor ) caused inhibition of tracer binding which diluted in parallel to synthetic
DYN suggesting the presence of substantial amounts of
DYN - like immunoreactivity (
irDYN ) in plasma . -
pubmed
Although use of Seppak
C18 cartridges resulted in reliable extraction of synthetic
porcine DYN from acidified plasma , we have not detected
irDYN in any plasma so far studied using this technique . -
pubmed
No significant change in the density of
[ 3H ] dihydroalprenolol - labeled
beta receptors was evident at this time point in either atrial or ventricular tissue , although
isoproterenol did decrease binding site density after 7 days of infusion . -
pubmed
These results indicate selective desensitization of cardiac
beta receptors without changes in
alpha-1 receptors by prolonged in vivo stimulation with
catecholamines . -
pubmed
The
cyclic AMP - dependent phosphorylation of
pyruvate kinase as a model in the study of regulation and turnover of phosphorylatable proteins . -
pubmed
The regrowth of resistant variants could be monitored by
luciferase assay of intracellular
ATP in cultures incubated with
gentamicin ( less than or equal to 2 micrograms / ml ) . -
pubmed
These findings of the specific regional localization suggest that
PP or
PP - LI may have a physiological role in the central nervous system . -
pubmed
Effects of substitution of the absolutely invariant
glutamine-4 and
phenylalanine-5 in
bovine pancreatic phospholipase A2 on enzymatic activity and substrate binding properties . -
pubmed
Aortic wall
cholesterol content ( on a weight basis ) correlated positively with serum
cholesterol concentration ( r = + 0.69 , P approximately 0.05 ) and negatively with the ratio of
HDL cholesterol to (
LDL plus
VLDL )
cholesterol ( double log plot : r = -0.79 , P less than 0.025 ) . -
pubmed
A study of
plasma neurophysins and action of
morphine ] . -
pubmed
Morphine administration was followed by a reduction of the diuresis and a slight elevation of plasmatic
neurophysins . -
pubmed
DOM and related
2,5 - dimethoxy-4 - alkylphenylisopropylamines : behavioral and
serotonin receptor properties . -
pubmed
The fundus preparation could not be used to determine pD2 values because all of the compounds were found to interact in an agonistic manner both with
serotonin and
PRT (
phenoxybenzamine resistant tryptamine ) receptors . -
pubmed
While each of these agents possesses a high
5 - HT receptor affinity , and while their behavioral effects might , therefore , involve a serotonergic component , the stimulus properties of these compounds are qualitatively dissimilar to those produced by the training dose of
5 - OMe DMT . -
pubmed
These data may identify a role for
carbonic anhydrase in
potassium excretion by newborn similar to that of adults . -
pubmed
Thus , in the current study , we investigated whether
BMS-186511 , a bisubstrate analogue inhibitor of
FT , would inhibit the malignant growth properties of a cell line established from
malignant schwannoma of an
NF1 patient . -
pubmed
BMS-186511 was found to specifically inhibit
FT , but not
geranylgeranyltransferase I , a closely related enzyme . -
pubmed
Concentration - response experiments showed that the response to exogenous
nitric oxide (
NO ; 10(-7)-10(-4 ) M ) was unaffected by
carbachol ( 10(-6 ) M ) preincubation , whereas responses to
vasoactive intestinal polypeptide (
VIP ) and
ATP were significantly reduced. -
pubmed
Neonatal exposure to xenobiotics alters adult hepatic
protein kinase C alpha levels and
testosterone metabolism : differential effects by
diethylstilbestrol and
phenobarbital . -
pubmed
The
PKC alpha levels generally correlated ( r = -.75 ) with
16 alpha - hydroxytestosterone formation in all samples . -
pubmed
A systematic mutational analysis of this 74 -
amino acid protein was performed and the effects on the potency of receptor binding and of
C5a - induced intracellular
calcium ion mobilization were measured . -
pubmed
Serum
creatinine and CrCL measurements during the
CsA challenge test predicted
post -
PTx levels : SCr 1.6 + /- 0.1 vs. 1.7 + /- 0.3 mg / dl , P = 0.48 , and CrCl 68 + /- 10 vs. 53 + /- 3 ml / min , P = 0.17 , respectively . -
pubmed
The mosquito
lipophorin had a hydrated density of 1.113 g / ml and contained 49 % lipid and 3.2 %
carbohydrate . -
pubmed
As model karyophiles we used
digoxygenin labelled
U1 snRNPs or
fluorescein labelled
U2 snRNPs . -
pubmed
The role of bound lipid and
transition metal in the formation of fluorescent advanced glycation endproducts by
human serum albumin . -
pubmed
In Group 1 mean serum levels of total
cholesterol ,
low - density lipoprotein cholesterol and
triglycerides were similar to those in mg / dl , p = 0.93 and 160 + /- 87 vs 155 + /- 76 , p = 0.74 , respectively ) while the mean serum level of
high - density lipoprotein cholesterol was higher in Group 2 ( 49 + /- 4 vs 46 + /- 4 mg / dl , p = 0.005 ) . -
pubmed
It was suggested that the down - regulation in
PRL binding to some extent at least resulted from receptor overstimulation caused by the
morphine - induced elevation in the concentrations of the endogenous hormone . -
pubmed
The present study indicates that the relaxant effect of these compounds in intestinal smooth muscle is mediated through
glibenclamide - sensitive
ATP - dependent K + channels . -
pubmed
We have monitored modifications of
GK mRNA expression after different periods of time in culture and we have studied the effect induced by
dexamethasone (
DEX ) treatment . -
pubmed
DEX treatment inhibits
GK mRNA expression in RIN cells in a dose - dependent and time - dependent manner . -
pubmed
Striated muscle contraction is elicited by the release of stored
calcium ions through
ryanodine receptor channels in the sarcoplasmic reticulum . -
pubmed
The rise in melanogenesis induced by
NO - generating compounds is associated with an increased amount of both
tyrosinase and
tyrosinase - related protein 1 . -
pubmed
Capillary electrophoresis ( CE ) with HeCd laser - induced fluorescence (
LIF ) detection and its application in forensic toxicology is demonstrated by the determination of
D - lysergic acid diethylamide (
LSD ) in blood . -
pubmed
Over 90 % frequently reported discussing the following topics with clients : home blood
glucose monitoring ,
hyperglycemia ,
hypoglycemia ,
insulin use , and diet . -
pubmed
We found that basal
irIL-1 beta secretion is significantly inhibited by
acetylcholine and
histamine and increased by
dopamine , while
dexamethasone ,
IL-4 and
IL-10 have no effect . -
pubmed
We have studied the thermodynamics of binding of ( i )
mannitol to enzyme
IImtl , ( ii ) the substrate analog
perseitol to enzyme
IImtl , ( iii )
perseitol to phosphorylated enzyme
IImtl , and ( iv )
mannitol to enzyme
IImtl treated with
trypsin to eliminate the cytoplasmic domains . -
pubmed
Sequence comparisons reveal a new
amino acid sequence motif (
CP ) , conserved among the ciliated protozoan
TERTs , and allow refinement of previously identified motifs . -
pubmed
Examination of the components of Exosurf revealed that two compounds ,
DPPC and
tyloxapol , showed inhibitory effects on
CAT expression . -
pubmed
The
p53 binding and transactivation of the
PTGF - beta promoter was enhanced by
etoposide , a
p53 activator , and was largely blocked by a dominant negative
p53 mutant . -
pubmed
Furthermore , expression of endogenous
PTGF - beta was remarkably induced by
etoposide in
p53 - positive , but not in
p53 - negative , cell lines . -
pubmed
Cyclic AMP - specific
phosphodiesterase and
succinate dehydrogenase activities were suppressed only with 5 micrograms dose , but with rising dose levels the effect was not so apparent . -
pubmed
Although the rise in
JH titers and biosynthesis and the fall in
JH esterase is correlated with ovarian development , application of
methoprene or
JH III in the absence of a carcass did not result in
vitellogenin uptake by the oocytes . -
pubmed
Although the role of
kainate receptors on sensory afferents is unknown , it has been hypothesized that presynaptic
kainate receptors may regulate
glutamate release in the spinal cord . -
pubmed
Here we show that
kainate , applied at low micromolar concentrations in the presence of the
AMPA - selective antagonist
( RS )- 4 -( 4 - aminophenyl )- 1 , 2 - dihydro-1 - methyl-2 - propyl - carbamoyl-6,7 - methylenedioxyphthalazine ++ + , suppressed spontaneous
NMDA receptor - mediated EPSCs in cultures of spinal dorsal horn neurons . -
pubmed
Interestingly , although the
glutamate receptor subunit 5 - selective
kainate receptor agonist
( RS )- 2 - alpha - amino-3 -( 3 - hydroxy-5 - tert - butylisoxazol-4 - yl ) propanoic acid (
ATPA ) ( 2 micrometer ) was able to suppress DRG - dorsal horn synaptic transmission to a similar extent as
kainate ( 10 micrometer ) , it had no effect on excitatory transmission between dorsal horn neurons . -
pubmed
Whereas DRG cell
kainate receptors were sensitive to both
kainate and
ATPA , most dorsal horn neurons responded only to
kainate . -
pubmed
Finally , in recordings from dorsal horn neurons in spinal slices ,
kainate and
ATPA were able to suppress
NMDA and
AMPA receptor - mediated EPSCs evoked by dorsal root fiber stimulation . -
pubmed
Together , these data suggest that
kainate receptor agonists , acting at a presynaptic locus , can reduce
glutamate release from primary afferent sensory synapses . -
pubmed
Asn49 is located in the type I beta turn formed by residues 49 - 52 , and is involved in extensive
hydrogen - bonding interactions between alpha helix 2 and the
rest of the N - terminal domain . -
pubmed
In addition , the mutated
enzyme exhibits : ( a ) an increase in the Km(
GSH ) value of about 6.5 - fold , and decrease in kcat value of about fourfold ; ( b ) viscosity - independent kinetic parameters ; ( c ) lower thermostability , and ( d ) increased susceptibility to proteolytic attack by
trypsin , when compared to the wild - type
enzyme . -
pubmed
Parasitologic ( xenodiagnosis , hemoculture , or both ) , histopathologic (
hematoxylin and eosin , and peroxidase - anti - peroxidase staining ) , and molecular (
polymerase chain reaction [ PCR ] followed by slot - blot hybridization ) tests were used in the analysis . -
pubmed
Heme oxygenase and
nitric oxide synthase are the enzyme systems responsible for generating
carbon monoxide and
nitric oxide , respectively . -
pubmed
Both
carbon monoxide and
nitric oxide share similar properties , such as the ability to activate soluble
guanylate cyclase to increase
cyclic GMP . -
pubmed
Although much is known about the
heme oxygenase /
carbon monoxide and
nitric oxide synthase /
nitric oxide pathways , how these two important systems interact is less well understood . -
pubmed
We report the identification of a
Ca ( 2 +)-responsive E - box element , CaRE2 , within
BDNF promoter III that binds
upstream stimulatory factors 1 and 2 (
USF1 / 2 ) and show that
USFs are required for the activation of
CaRE2 - dependent transcription from
BDNF promoter III . -
pubmed
We find that the transcriptional activity of the USFs is regulated by
Ca ( 2 +)-activated signaling pathways in neurons and that the
USFs bind to the promoters of a number of neuronal activity - regulated genes in vivo . -
pubmed
Transcription factors , guidance
molecules , extracellular matrix proteins ,
neurotrophic factors , and cell
death - regulating factors of the
Bcl-2 family and caspases , have all been reported to be involved in the processes of formation of a precise retino - collicular map , and regulation of developmental cell
death . -
pubmed
Testosterone binding to
bovine serum albumin : effect of pH. -
pubmed
THE RELATION OF
GAS - CHROMATOGRAPHIC RETENTION TIME TO THE STRUCTURE OF
STEROIDS AND DERIVATIVES . -
pubmed
To test the stability of
S2 , we constructed a series of " zippered " dimeric
smooth muscle myosin II compounds , containing a high - melting temperature 32 -
amino acid GCN4
leucine zipper in the S2 rod beginning 0 , 1 , 2 , or 15 heptads from the head - rod junction . -
pubmed
We then assessed the ability of these and wild - type
myosin to bind strongly via two heads to an
actin filament by measuring the fluorescence quenching of
pyrene - labeled
actin induced by
myosin binding . -
pubmed
We find that wild - type and zippered
heavy meromyosin (
HMM ) are able to bind by both heads to
actin under both
nucleotide - free and saturating
ADP conditions . -
pubmed
The proximate cause of the prepubertal quiescence in this neuroendocrine axis is the arrest or restraint of the pulsatile mode of hypothalamic
GnRH release by a neurobiological brake that holds in check release of this decapeptide , without seeming to down - regulate the transcriptional activity of the gene encoding
GnRH (
GnRH - I ) . -
pubmed
The genetic and physiological factors ( with a particular emphasis on
leptin ) that time the application and duration of the prepubertal brake on
GnRH release are also considered . -
pubmed
There was a direct correlation between BM and spleen uptake of
FDG with increasing WBC , which was stronger than the inverse correlation seen with
Hb ( the lower the
Hb the greater the uptake ) . -
pubmed
CONCLUSION : There is a correlation between hematologic parameters such as
Hb , WBC and platelet counts and the uptake of
FDG in BM and spleen in PET imaging . -
pubmed
The expression of
HIF-1alpha ,
endothelin-1 ,
inducible nitric oxide synthase , and
vascular endothelial growth factor mRNA was highest during the active stage of
ulcer healing , and
endothelin-1 ,
vascular endothelial growth factor protein levels and
nitric oxide were higher during the healing stage . -
pubmed
Large scale molecular dynamics simulation of native and mutant
dihydropteroate synthase -
sulphanilamide complexes suggests the molecular basis for
dihydropteroate synthase drug resistance . -
pubmed
Dihydropteroate synthase (
DHPS ) has been targeted by
sulphonamide inhibitors for the past 60 years and has developed different
amino acid mutations to survive
sulpha drug action . -
pubmed
The
amino acid residues
serine at position 213 ( S213 ) and
lysine at position 218 ( K218 ) , which are present in close proximity to the
histidine - rich motif
II of
Mucor rouxii fatty acid Delta ( 6 )- desaturase isoform II , were targeted for studying structure - function relationships using site - directed mutagenesis . -
pubmed
rouxii
Delta ( 6 )- desaturase therefore has potential in specifically altering substrate utilization for production of desired
fatty acids . -
pubmed
In the repeated batch fermentations of the shake flasks , an optimum level of
enzyme was maintained for 9 days using
calcium alginate immobilized cells . -
pubmed
Forskolin - induced
cAMP formation was inhibited by
[ D - Ala(2 ) ,
N - Me - Phe(4 ) ,
Gly ( 5 )- ol ]- enkephalin (
DAMGO 1 microM ) , a specific
MOR agonist . -
pubmed
Annexin V ,
RGS4 and CREB genes showed pronounced increase in expression after stimulation with
DAMGO . -
pubmed
Quantitative RT - PCR confirmed that
DAMGO increased mRNA levels of
Annexin V , an apoptosis - induced gene . -
pubmed
Sera were assayed for
progesterone ( P4 ) ,
prostaglandin F2alpha metabolite (
PGFM ) , and
luteinizing hormone (
LH ) concentrations . -
pubmed
UFAs homeostasis in many organisms is achieved by feedback regulation of
fatty acid desaturase gene transcription through signalling pathways that are governed by sensors embedded in cellular membranes . -
pubmed
During stimulation
phospho -
JNK levels were significantly increased at 3 , 6 , and 12 h after 0.1 or 0.5 microM
arsenite exposure . -
pubmed
Phospho -
ERK1/
2 levels were increased with different concentrations ( 0.1 - 10 microM ) of arsenite at 6 , 12 , and 24 h. Blocking of JNK pathway with 20 microM SP600125 or ERK1/2 by 100 microM PD98059 significantly inhibited biphasic effect of arsenite in cells . -
pubmed
As for the
pneumococcal infection in patient with good immunological response , good clinical effect might be obtained by
panipenem /
betamipron (
PAPM / BP ) 500 mg ,
imipenem /
cilastatin (
IPM /
CS ) 500 mg ,
meropenem (
MEPM ) 500 mg and
biapenem (
BIPM ) 300 mg , b.i.d . -
pubmed
, or
IPM /
CS 500 mg ,
MEPM 500 mg , t.i.d . -
pubmed
At the bases of glands ,
intrinsic factor immunoreactive cells also were costained for
5 - bromo-2 '- deoxyuridine , suggesting their re - entry into the cell cycle . -
pubmed
Inspectors from a public health center treating the housewife 's complaint quantified
formaldehyde (
FA ) in high concentration exceeding Japanese national guideline of
FA in some rooms of the house . -
pubmed
The
PETRA cross - linked polymers showed the best recognition (
MIP 2- , alpha=1.71 ) and selectivity (
MIP 2 + , alpha'=5.58 ) capabilities . -
pubmed
Recently , it has been shown that
TNF -[ alpha ] may be a novel target for the treatment of acute
pancreatitis ; but the role of
thalidomide , an immunomodulatory agent that inhibits
TNF -( alpha ) and angiogenesis , has not been investigated so far . -
pubmed
The study examined the cross - sectional relationship between
carbohydrate , GI ,
GL and risk of the
metabolic syndrome . -
pubmed
Dietary
carbohydrate , GI and
GL were determined by an interview - administered FFQ . -
pubmed
The risk of developing the
metabolic syndrome was positively related to dietary
carbohydrate ( P for trend = 0.03 ) , GI ( P for trend = 0.03 ) and
GL intakes ( P for trend = 0.02 ) in women after adjusting for potential confounding variables . -
pubmed
Among the components of developing the
metabolic syndrome , the risk of high
TAG and low
HDL - cholesterol were positively related to high GI and
GL intakes in women . -
pubmed
The risk of developing the
metabolic syndrome was considerably higher in the highest quintiles of
carbohydrate ( OR 6.44 ; 95 % CI 2.16 , 19.2 ) , GI ( OR 10.4 ; 95 % CI 3.24 , 33.3 ) and
GL intakes ( OR 6.68 ; 95 % CI 2.30 , 19.4 ) than in the lowest quintiles among women with a
BMI & gt;/= 25 kg / m2 . -
pubmed
However , there was no difference in risk across quintiles of
carbohydrate , GI and
GL among women with a
BMI & lt ; 25 kg / m2 . -
pubmed
Acetylcysteine therapy for
chronic hepatitis C : are its effects synergistic with
interferon alpha ? -
pubmed
Group A was treated with lymphoblastoid
IFNalpha 6MU three times a week for 6 months ; group B received the same schedule of
lymphoblastoid IFNalpha as group A plus
acetylcysteine 1200 mg / day per os in two administrations , and group C received only
acetylcysteine 1200 mg / day per os in two administrations . -
pubmed
Hypercatabolic syndrome (
HS ) is a biochemical state characterized by increased circulating catabolic hormones ( eg ,
cortisol ,
catecholamines ) and inflammatory
cytokines ( eg ,
tumor necrosis factors ,
interleukin-1beta ) , and decreased anabolic
insulin effects with consequent
insulin resistance . -
pubmed
The local ejection of
glutamate over RS cells elicited excitatory responses that were only slightly depressed by 5 -
HT . -
pubmed
Second , in experiments with animals , it was found that
SPS reduced serum
triacylglycerol (
TG ) elevation induced by high - fat diets . -
pubmed
SMN2 contains four
CpG islands which present highly conserved methylation patterns and little interindividual variations in
SMN1 - deleted
SMA patients . -
pubmed
The comprehensive analysis of
SMN2 methylation in patients suffering from severe versus mild
SMA carrying identical
SMN2 copy numbers revealed a correlation of
CpG methylation at the positions -290 and -296 with the disease severity and the activity of the first transcriptional start site of
SMN2 at position -296 . -
pubmed
We identified
histone deacetylase (
HDAC ) inhibitors including
vorinostat and
romidepsin which are able to bypass
SMN2 gene silencing by DNA methylation , while others such as
valproic acid and
phenylbutyrate do not , due to
HDAC isoenzyme specificities . -
pubmed
The
phosphoprotein - bound beads suspended in a sample - loading
dye solution were subjected to
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis , followed by Western blotting . -
pubmed
Remission was induced with
prednisolone and intravenous
cyclophosphamide , followed by maintenance therapy with
azathioprine , during which
MPO - ANCA results became negative . -
pubmed
RESULTS : A panel of genes , proteins and metabolites , including
PGE2 and
TNF - alpha , were identified that describe a
diclofenac - response network ( 68 genes in PBMC , 1 plasma protein and 4 plasma metabolites ) . -
pubmed
Novel candidate markers of inflammatory modulation included PBMC expression of
annexin A1 and
caspase 8 , and the
arachidonic acid metabolite
5,6 - DHET . -
pubmed
However , the specific method of measuring
calcium in serum using
CPZ - III has not been established because of the difficulty of avoiding the interaction between
CPZ - III and
albumin . -
pubmed
METHODS : In this study , we found that the non - specific reaction between
CPZ - III and
albumin could be controlled and
calcium in serum could be specifically detected using
CPZ - III combined with
vanadate . -
pubmed
The assay results correlated well with those of the
Arsenazo - III (
2,7 - bis ( 2 - arsonophenylazo )- 1,8 - dihydroxy-3,6 - naphthalenedisulphonic acid ) method ( slope = 1.067 ; intercept = -0.120 ; r = 0.989 ; Sy / x = 0.036 mmol / L ) ,
o - cresolphthalein complexone method ( slope = 0.911 ; intercept = 0.186 ; r = 0.988 ; Sy / x = 0.035 mmol / L ) ,
amylase enzymatic method ( slope = 0.981 ; intercept = 0.072 ; r = 0.989 ; Sy / x = 0.036 mmol / L ) and inductively coupled plasma emission spectroscopy method ( slope = 0.955 ; intercept = -0.001 ; r = 0.979 ; Sy / x = 0.048 mmol / L ) . -
pubmed
The
Her2 /
neu receptor sites were labelled by immunofluorescence using conventional fluorescent dyes (
Alexa conjugated antibodies ) . -
pubmed
In a preliminary batch adsorption experiment ,
H4 was evaluated to be highly efficient in the removal
Hg ( II ) from synthetic aqueous solution . -
pubmed
Drain blocking led to increases in the
E4 : E6 ratio , and declines in specific absorbance , suggesting that
DOC released from blocked drains consisted of lighter , less humic and less decomposed
carbon . -
pubmed
Whilst concentrations of
DOC showed slight increases in drains and streams after blocking , instantaneous yields of both
DOC and POC declined markedly in streams over the first year
post - restoration . -
pubmed
MAJOR CONCLUSIONS :
Inositol is a building block of
phosphatidylinositol (
PI ) , which is further elaborated to become PIMs , LM and
LAM . -
pubmed
Several additional control mechanisms must exist to control the complex biosynthetic pathways of
PI , PIMs , LM and
LAM . -
pubmed
GENERAL SIGNIFICANCE : Mycobacteria have evolved a complex network of
inositol metabolism . -
pubmed
Diagnostic value of
progesterone level and
progesterone /
estradiol ratio on the day of
human chorionic gonadotropin administration . -
pubmed
Our data indicate that
resveratrol modulates the effect of
MED28 on cellular migration , presumably through the
EGFR /
phosphatidylinositol 3 - kinase (
PI3 K ) signaling pathway , in
breast cancer cells . -
pubmed
At2g34630 is the main -- if not sole -- contributor to
solanesyl diphosphate synthase activity required for the biosynthesis of
ubiquinone , as demonstrated by the dramatic ( 75 - 80 % ) reduction of the
ubiquinone pool size in corresponding RNAi lines . -
pubmed
Overexpression of
At2g34630 gave up to a 40 % increase in
ubiquinone content compared to wild - type plants . -
pubmed
AIM : Coronary angiography is usually done with
heparin . -
pubmed
With the increasing of
PEHD concentration , the expression of
caspase-8 ,- 9 ,- 3 and
PARP protein increased . -
pubmed
In this work , we present a combined scanning tunneling microscopy , secondary ions mass spectrometry , and magnetotransport study to understand the atomistic doping process of
Ge by
P2 molecules . -
pubmed
Harnessing the one - dimer footprint of
P2 molecules on the
Ge(001 ) surface , we achieved the incorporation of a full P monolayer in
Ge using a relatively low process temperature . -
pubmed
JDS - chromium -
insulin ( CRI)-003 is a novel form of
insulin that has been directly conjugated with
chromium (
Cr ) instead of
zinc . -
pubmed
Plasma
glucose and
insulin were measured . -
pubmed
Insulin tolerance tests at week 7 showed that both
insulin treatments significantly reduced
glucose concentrations and increased
insulin levels compared with the Sham group , CRI significantly reduced
glucose at 4 and 6 h relative to Reg - In ( P & lt ; 0.05 ) , suggesting the effects of CRI on reducing
glucose last longer than Reg - In . -
pubmed
cholerae resistance against
bile salts , perhaps due to environmental regulation of
AmiB or its activators . -
pubmed
Efficacy and safety of
nadifloxacin for
bacterial skin infections : results from clinical and
post - marketing studies . -
pubmed
METHODS : This article presents the results of one
post - marketing surveillance ( PMS ) and three randomized , open , non - blinded , multi - centric clinical studies that compared
nadifloxacin with
mupirocin and
framycetin , and
nadifloxacin with
fusidic acid . -
pubmed
Intracellular
Ca ( 2 + ) level was detected by Hitachi - 850 fluorospectrophotometer ,
calcineurin (
CaN ) activity was measured by colorimetric method , the expression of
CaN protein was observed with immunocytochemistry , and the proto - oncogene c - fos mRNA expression was assessed with reverse transcription
polymerase chain reaction ( RT - PCR ) . -
pubmed
However ,
actin assembly , spindle structure and chromosome alignment were not disrupted after exposure to 50 muM
AFB1 . -
pubmed
AFB1 treatment ( 50 muM ) also induced oxidative stress and further led to autophagy , as shown by accumulation of reactive
oxygen species , up - regulated
LC3 protein expression and increased mRNA levels of
ATG3 ,
ATG5 and
ATG7 . -
pubmed
Annexin V -
FITC staining assay revealed that
AFB1 treatment ( 50 muM ) resulted in oocyte early apoptosis , which was confirmed by increased
Bak ,
Bax ,
Bcl - xl mRNA levels . -
pubmed
In addition ,
TMC reduced protein expression of
Akt ,
MMP-2 and
MMP-9 and enhanced
E - cadherin and
TIMP-1 protein expression , which are known to regulate cell adhesion , migration and invasion . -
pubmed
Here , we combine fluorescence correlation spectroscopy and
acrylodan fluorescence screening to study the stoichiometry and structural features of
tau -
tubulin assemblies . -
pubmed
The DLS ,
TEM ,
AFM , FT - IR and Powder - XRD studies demonstrate strong binding of
creatinine and
urea to the nanoparticles . -
pubmed
Real - time PCR was applied to determine any variation in levels of expression of the
serotonin (
Slc6A4 or
Sert ) and
dopamine transporter (
Slc6A3 or
Dat ) genes . -
pubmed
In order to better understand the regulation and role of
NF - kappaB in
acute kidney injury we explored the expression of
NF - kappaB - related genes in experimental
acute kidney injury induced by a
folic acid overdose . -
pubmed
Kidney
NFkappaBiz mRNA upregulation and protein downregulation was also observed in
acute kidney injury induced by
cisplatin or unilateral
kidney injury resulting from
ureteral obstruction . -
pubmed
FAA was also positively related to the level of restriction and inversely to
leptin ,
insulin ,
TNF - alpha and IGF-1 . -
pubmed
The Secreted Enzyme
PM20D1 Regulates Lipidated
Amino Acid Uncouplers of Mitochondria . -
pubmed
We demonstrate that
PM20D1 is a bidirectional enzyme in vitro , catalyzing both the condensation of
fatty acids and
amino acids to generate
N - acyl amino acids and also the reverse hydrolytic reaction . -
pubmed
N - acyl amino acids directly bind mitochondria and function as endogenous uncouplers of
UCP1 - independent respiration . -
pubmed
Detection of reactive
oxygen species ( ROS ) and measurement of mitochondrial membrane potential (
MMP ) were performed by flowcytometry . -
pubmed
Fasting blood
glucose ,
insulin and
HBA1C level and different oxidative and anti - oxidative stress parameters were measured and pancreatic tissue sections were stained with eosin -
haematoxylene . -
pubmed
Nicotine also caused oxidative stress in pancreatic tissue as evidenced by increased
nitric oxide and
malondialdehyde level and decreased
superoxide dismutase ,
catalase and
reduced glutathione level . -
pubmed
Despite its proven benefits , half of the patients treated with
imatinib show
disease progression within 2 years due to secondary resistance mutations in
KIT . -
pubmed
Pre - existing
cancer cells with genetic alterations promoting apoptosis resistance may serve as a basis whereby
cancer cells with critical mutations , such as secondary
KIT mutations , can establish full
imatinib resistance . -
pubmed
Antral follicle count ( AFC ) ( p = 0.001 ) , total
FSH stimulating dose ( p = 0.0001 ) , and peak serum
estradiol level ( p = 0.0001 ) were associated with spindle angle grouping . -
pubmed
According to the ratio of
GAPDH /
CS and the
LDH level the gasterozooids prefer the anaerobic glycolytic pathway whereas in the sexual hydranths relatively more substrate is supplied at the disposal of the
citrate cycle . -
pubmed
Significant sequential reductions in
P2Y12 reaction units were noted , in the following order :
clopidogrel , low - dose
ticagrelor , and standard - dose
ticagrelor ( ANCOVA , p<0.001 ) . -
pubmed
After
PSA ( antigen ,
Ag ) was added into the system , a noncompetitive immune reaction was happen between
Ab * and
Ag to form an immune complex (
Ag - Ab * ) . -
pubmed
We hypothesized that
beta - AR blockade with
propranolol would restore endothelial function and vascular
insulin signaling in
obesity , associated with an anti - inflammatory effect . -
pubmed
Protective effect of
propranolol administration in endothelial function was associated with increased
nitric oxide (
NO ) production and phosphorylation of
Akt (
Ser473 ) and
eNOS (
Ser1177 ) , but with reduced
phospho -
IRS-1(
Ser307 ) and
phospho -
ERK1 / 2 (
Thr202 /
Tyr204 ) . -
pubmed
In conclusion , our results suggest that
beta - AR blockade with
propranolol is effective to prevent the
endothelial dysfunction , vascular IR and pro - inflammatory state displayed in HFD - induced
obesity , independent of changes in
body weight . -
pubmed
We studied the growth of
Ge layers on
Au ( 111 ) under ultra - high vacuum conditions from the submonolayer regime up to a few layers with Scanning Tunneling Microscopy (
STM ) , Direct Recoiling Spectroscopy (
DRS ) and Low Energy Electron Diffraction ( LEED ) . -
pubmed
The high surface sensitivity of
TOF -
DRS allows us to confirm the coexistence of
Au and
Ge atoms in the top layer for all stages of growth . -
pubmed
Here ,
asialoglycoprotein receptor (
ASGP - R)-targeting
paclitaxel - conjugated gold nanoparticles ( Gal /
PTX - GNPs ) are fabricated as a demonstration to realize the precise treatment of
liver cancer . -
pubmed
The drug level in
tumor versus liver of Gal /
PTX - GNPs is 121.0 % at 8 h
post injection , a 15.7 - fold increase in the
tumor specificity compared to that of GNPs conjugated with
PTX only . -
pubmed
Intra - cerebral
cannabidiol infusion - induced neuroprotection is partly associated with the
TNF - alpha /
TNFR1 /
NF - kB pathway in transient focal
cerebral ischaemia . -
pubmed
Down - regulation of
TNF - alpha ,
TNFR1 , and
NF - kB expression was also observed by
CBD . -
pubmed
CONCLUSION : The results achieved in this study support the idea that
CBD has a cerebroprotective effect ( partly through suppression of
TNF - alpha ,
TNFR1 , and
NF - kB ) on
ischaemic injury . -
pubmed
Non - fasting total
cholesterol ,
triglycerides and
HDL -
Cholesterol (
HDL - C ) were measured . -
pubmed
25 ( OH ) D is inversely correlated with
triglycerides and non -
HDL - C in the total population and in boys and girls ( p & lt ; 0.0001 for all ) , and positively correlated with
HDL - C in the total population and in boys but not in girls ( p = 0.001 , p & lt ; 0.0001 and p = 0.1 , respectively ) . -
pubmed
In a multilinear regression analysis , in the overall population ,
25 ( OH ) D is independently associated with sex , season , school 's SES , BMI ,
triglycerides and non -
HDL - C. In boys , the association with
BMI and season is non - significant . -
pubmed
We demonstrated that
lysine 830 of
BRCA1 is a preferential acetylation site by
pCAF and tested its function in embryonic stem ( ES ) cells by changing
lysine 830 to
arginine using a transcription activator - like effector nuclease ( TALEN ) system . -
pubmed
These data suggest that acetylation of
BRCA1 on
lysine 830 activates
BRCA1 function at the intra - S checkpoint after DNA damage . -
pubmed
Analysis of
mercury adsorption at the
gibbsite - water interface using the
CD - MUSIC model . -
pubmed
In the present study , simulation experiments on macroscopic
Hg ( II ) sorption by
gibbsite ( alpha -
Al ( OH ) 3 ) , a representative
aluminum ( hydr ) oxide mineral , were performed using the charge distribution and multi - site complexation (
CD - MUSIC ) approach with 1 - pK triple plane model ( TPM ) . -
pubmed
The model successfully incorporated the presence of inorganic ligands at the dominant edge ( 100 )
face of
gibbsite with consistent surface species , which was evidenced by molecular scale analysis . -
pubmed
Annexin V /
propidium iodide and
terminal deoxynucleotidyl transferase - mediated
dUTP nick end labeling assay was used to detect
BLE - induced apoptosis . -
pubmed
CONCLUSIONS : :
BLE induced
NPDF apoptosis and ECM degradation through a mitochondrial - mediated pathway and in a ROS - dependent manner . -
pubmed
Here , we determined whether chronic intermittent access (
CIA ) to
alcohol during adolescence / early adulthood or during adulthood would alter or be associated with auditory - cued conditioned fear levels using an extended training fear incubation procedure . -
pubmed
Interventions of interest are open surgery ( ie , saphenofemoral or high ligation (
HL ) with stripping ) and endovenous ablations ( ie , EVLA , RFA , EVSA , MOCA ,
cyanoacrylate injection and UGFS ) . -
pubmed
TH did not confer protection against
flurothyl seizure susceptibility at
P18 in this model . -
pubmed
The ensemble networks enabled accurate predictions for full - thickness reference properties ( PG content : rhoin vitro ,
Val= 0.691 , rhoin vivo= 0.676 ;
collagen orientation angle : rhoin vitro ,
Val= 0.626 , rhoin vivo= 0.574 ) from NIR spectral data . -
pubmed
UPC2A deletion also increased susceptibility to both
triazoles and
tetrazoles , but to a lesser extent than
PDR1 deletion . -
pubmed
To further investigate the mechanism by which
DNA - PKcs promotes
cSCC progression ,
TGF - beta1 and the
TGF - beta receptor (
TGF - betaR ) I / II dual inhibitor
LY2109761 were used to examine whether
DNA - PKcs participates in
TGF - beta1 /
Smad signaling . -
pubmed
Background :
Methylenetetrahydrofolate reductase (
MTHFR ) has a key role in converting
homocysteine to
methionine so its deficiency might cause
homocysteine accumulation and
arterial destruction . -
pubmed
Serum
homocysteine and
MDA level as well as
MTHFR activity were measured manually . -
pubmed
Also , the results indicate that
MTHFR activity in the two groups was statistically different ( p - value < 0.001).Conclusion : It can be concluded that the regulation of
homocysteine and its metabolic enzymes plays a significant role in controlling blood pressure and consequently in the pathophysiology of
Preeclampsia and it can be a goal for prevention and treatments . -
pubmed
Suberoylanilide hydroxamic acid (
SAHA ) , a clinically used
HDAC inhibitor , was then intraperitoneally and intrathecally injected to rescue the increased expression levels of
HDAC1 and
HDAC2 . -
pubmed
RESULTS :
TCI caused rapid and long - lasting increased expression of
HDAC1 /
HDAC2 in glial cells of the spinal dorsal horn and dorsal root ganglia . -
pubmed
Inhibiting
HDACs by
SAHA not only reversed
TCI - induced upregulation of
HDACs but also inhibited the activation of glial cells in the spinal dorsal horn and dorsal root ganglia , and relieved
TCI - induced
mechanical allodynia . -
pubmed
Moreover , we found that
SAHA administration could inhibit
GSK3beta activity in the spinal dorsal horn and dorsal root ganglia , which might contributed to the relief of BCP . -
pubmed
CONCLUSION : Our findings suggest that
HDAC1 and
HDAC2 are involved in the glia - mediated neuroinflammation in the spinal dorsal horn and dorsal root
ganglia underlying the pathogenesis of
BCP , which indicated that inhibiting
HDACs by
SAHA might be a potential strategy for
pain relief of BCP . -
pubmed
The Role of
Ca2 + -
NFATc1 Signaling and Its Modulation on Osteoclastogenesis . -
pubmed
Cytosolic
calcium binds to
calmodulin and subsequently activates
calcineurin , leading to
NFATc1 activation , a master transcription factor required for osteoclast differentiation . -
pubmed
Thus , in this review , we summarized the modulation of the
NFATc1 pathway through various compounds that regulate
calcium signaling and the
calcium influx machinery . -
pubmed
Amorphous and crystalline
dihydrate formulations showed no change in aerosolization parameters ( emitted dose and
FPF ) upon storage for three months . -
pubmed
Following aerosolization , islet cell vitality and function were assessed via
Calcein AM and
propidium iodide as well as
insulin production after
glucose exposure . -
pubmed
,
gpx1b ,
gpx4a ,
gpx7 and
sod3a ) involved in detoxifying cellular reactive
oxygen species were downregulated significantly . -
pubmed
Among non - fermenting Gram - negative bacilli ,
fluoroquinolone resistance is mostly due to
amino acid substitutions in localized regions of the targets (
GyrA ,
GyrB ,
ParC and
ParE ) named
QRDRs , but also to efflux . -
pubmed
METHODS : The putative QRDRs of
GyrA ,
GyrB ,
ParC and
ParE were sequenced in 62 clinical isolates , and in vitro one - step mutants obtained after exposure to
fluoroquinolones . -
pubmed
RESULTS : We detected seven substitutions in
QRDRs ( Q83L / S84P / D87N / D87 G for
GyrA , Q480P for
GyrB , T395A / K525Q for
ParE ) , all in nine of the 27 clinical isolates with
ciprofloxacin MIC > = 16 mg / L , whereas none among the in vitro mutants . -
pubmed
Substitutions in
AxyT associated with overexpression of
AxyEF -
OprN were also detected in seven clinical
strains with
ciprofloxacin MIC > = 16 mg / L. CONCLUSIONS : Target alteration is not the primary mechanism involved in
fluoroquinolone resistance in Achromobacter . -
pubmed
BACKGROUND :
Infections caused by Enterobacteriaceae are mainly treated with the
beta - lactam antibiotics , nevertheless , the emergence of species with plasmid - borne
beta - lactamases has decreased the efficacy of these antibiotics . -
pubmed
Among 35
cefoxitin resistant isolates , 18 ( 51.4 % ) identified as carrying
AmpC genes by multiplex PCR . -
pubmed
Within
AmpC beta - lactamase genes ,
DHA gene was the predominant gene ( 15 , 42.3 % ) . -
pubmed
Conclusions : Administration of
insulin plus
metformin in
type-1 diabetes could modulate the function of
CYP2D1 to the observed levels in the control group and made it clearer to predict the fate of drugs that are metabolized by this enzyme . -
pubmed
Methods : Amorphous
zirconia was deposited using an atomic layer deposition reactor ( Atomic Classic ,
CN1 , Hwaseong , Korea ) on
titanium discs . -
pubmed
The purified
protease yielded only 1 line in the
SDS - polyacrylamidegel - electrophoresis , in the
gelatin - polyacrylamidegel - electrophoresis and in the double immuno - diffusion test ( fig . -
pubmed
The
protease - activity was completely inhibited in the presence of
EDTA , partially inhibited by
Cu2 + and
Zn2 + and increased by
Mn2 + ( table 3 ) . -
pubmed
The actions of neuroleptic drugs and putative
serotonin receptor antagonists on
LSD and
quipazine - induced reductions of brain
5 - HIAA concentrations . -
pubmed
The formation of the unusual species of
lecithin in
tumor tissues was discussed in relation to the impairing in the
enzyme systems involved in
lecithin synthesis . -
pubmed
[ Serum
glucose determination using the
hexokinase and
glucose dehydrogenase technic on the autoanalyzer ] . -
pubmed
The prevalence of
hepatitis - B antigen (
HB S Ag ) and its antibody (
Anti - HB S ) among London hemophiliacs and blood donors from London and two tropical areas . -
pubmed
The prevalence of
hepatitis - B antigen (
HB s AG ) and its antibody (
anti - HBs ) among 49 London hemophiliacs was compared with that among women attending an antenatal clinic and healthy blood donors from London and from two parts of the tropics ( Mauritius and Zambia ) . -
pubmed
Calcium channels and
nifedipine inhibition of
serotonin - induced
[ 3H ] thymidine incorporation in cultured cerebral smooth muscle cells . -
pubmed
Passaged cultures ( 10 - 30 passages ) that expressed
serotonin receptors were studied using
[ 3H ] thymidine incorporation . -
pubmed
When tested in quiescent medium ,
serotonin potently stimulated
[ 3H ] thymidine incorporation ( EC50 of 31 nM ) by as much as 400 % at 24 h. The number of cells was not significantly increased at 24 or 48 h. At concentrations of 10(-8)-10(-5 ) M 5 -
HT ,
[ 3H ] thymidine uptake was reduced 40 - 50 % by the
dihydropyridine Ca2 + channel blocker ,
nifedipine ( 1 microM ) . -
pubmed
To demonstrate a possible mechanism for the sensitivity to
nifedipine ,
Ca2 + currents were measured using the whole cell patch
clamp technique . -
pubmed
The cells expressed
dihydropyridine - sensitive L - type
Ca2 + channels , but not other subtypes of
Ca2 + channels , as indicated by the kinetic and voltage - dependent characteristics of the current and by the stimulatory effect of
Bay K 8644 . -
pubmed
Significant reductions of
estradiol ( p less than 0.01 ) and
CA125 ( p less than 0.001 ) levels after three courses of chemotherapy were observed . -
pubmed
In experiments on agonist - induced
PA ,
TMS inhibited
PA and
ATP secretion 5 - fold more strongly than
DMS or
SPN . -
pubmed
Tumor cell ( BL6 melanoma)-induced
PA and
ATP secretion were also strongly inhibited by
TMS , but not by
DMS or
SPN . -
pubmed
Unlike
ADP- or thrombin - induced
PA , BL6 cell - induced
PA was not inhibited by
Calphostin - C ( a potent
protein kinase C inhibitor ) or
cilostazol ( a potent inhibitor of
PA based on inhibition of
cyclic AMP phosphodiesterase ) . -
pubmed
The nucleotide sequence of the 5 ' portion of this gene revealed a coding sequence for a 60 residue propeptide containing two
Lys - Arg amino acid pairs that have been identified as sites for
peptidase processing of several exported peptides and proteins . -
pubmed
The final
Lys - Arg site occurs at the junction with the mature extracellular form of the
acid protease . -
pubmed
Four 25 - nt long
oligonucleotides containing dA and dT (
D1 ,
D2 ,
D3 , and
D4 ) which are capable of forming parallel - stranded ( ps ) or antiparallel - stranded ( aps ) duplexes have been synthesized [ Rippe , K. , Ramsing , N. B. , & amp ; Jovin , T. M. ( 1989 ) Biochemistry 28 , 9536 - 9541 ] . -
pubmed
A similar result was observed with
KMnO4 and
DEPC , although with the latter reagent the modification pattern of the labeled strands
D1 * and
D4 * was slightly different for the parallel than for the antiparallel duplex . -
pubmed
It is possible , therefore , that the
FAE generally performs class II - restricted transport and presentation to T cells of
antigens which have been adequately processed in the gut lumen . -
pubmed
Sphingomyelin was not accumulated , while lysosomal
sphingomyelinase was dramatically inhibited . -
pubmed
Sodium dodecyl sulfate -
polyacrylamide gel electrophoresis and two - dimensional gel electrophoresis showed that the rod portion of the released structures consisted of the 30 - kilodalton
FlgG protein . -
pubmed
[ DNA - duplexes with
phosphoamide bond : interaction with
restriction endonucleases EcoRII and
SsoII ] . -
pubmed
We studied the interaction of
EcoRII and
SsoII restriction endonucleases with synthetic DNA duplexes , containing 3'N----5'P and 3'P----5'N
phosphoamide internucleotide bonds in one of the cleavage points . -
pubmed
The presence of
phosphoamide bonds was found to reduce the rate of cleavage of the natural strand by
EcoRII and to have no influence in case of
SsoII . -
pubmed
In the presence of
Mg2 + ions the equilibrium association constant of the
enzyme - substrate complex is 3 - fold reduced , and the dissociation rate constant of the complex is increased by 1.5 times . -
pubmed
Routine detection of
IgG oligoclonal bands and free light chains patterns by means of
agarose isoelectric focusing and avidin -
biotin - peroxidase complex staining in
multiple sclerosis unconcentrated cerebrospinal fluid . -
pubmed
Administration of a
thiol radioprotector
beta - mercaptoethylamine resulted in alteration of kinetic properties of
tryptophane hydroxylase expressed as a decrease in the optimal concentration of the substrate ; this decrease was more distinct in liver and small intestine as compared with mast cells . -
pubmed
Isotherms of the adsorption of
acridine dyes on
tRNA . -
pubmed
The results presented indicate that
AMINO , like
AMI , inhibits NA uptake and is a
5 - HT antagonist . -
pubmed
When combining the
carbohydrate detection method with the
peroxidase -- antiperoxidase (
PAP ) method used for immunocytochemical detection of the various pancreatic islet hormones , paraffin sections reveal that glycogen is primarily localized in granulated B cells ; the degranulated B cells also contain glycogen , though in smaller amounts . -
pubmed
In contrast , the islet cells containing
somatostatin ,
glucagon and
pancreatic polypeptide are nearly free of glycogen . -
pubmed
[ Feasibility of a
cyclophosphamide methotrexate and
5 - fluorouracil regime intravenously administered with and without
granulocyte - colony stimulating factor in surgically treated
breast carcinoma ] . -
pubmed
The aim of this study was to evaluate the feasibility of
CMF 1.8 - 28 regimen , with all three drugs administered intravenously (
IV ) , and to compare the
hematologic toxicity of this regimen with or without
G - CSF . -
pubmed
Treatment with
G - CSF after
CMF 1.8 regimen resulted in a significantly WBC 's earlier nadir ( average day of nadir 14 vs 17 ; p = 0.0005 ) , while there was no difference in the average values of WBC at the nadir . -
pubmed
CMF 1.8 with
IV cyclophosphamide is a feasible regimen with and without
G - CSF and can be used in adjuvant setting instead of " classic "
CMF in order to improve the low compliance observed when
cyclophosphamide is given by mouth . -
pubmed
Sequencing of the
FBN1 gene revealed a heterozygous C to T transition at nucleotide 8176 resulting in the substitution of a
tryptophan for an
arginine ( R2726W ) , at a site immediately adjacent to a consensus sequence recognized by a cellular
protease . -
pubmed
The positional distribution of the
thioic acid ester in the lipid was determined by
beta - galactosidase ,
sulphur -
oxygen exchange reaction using
silver nitrate , and
lipase hydrolysis of the
diacylglycerol derived from the lipid . -
pubmed
Serum
cortisol was determined several times a day before and just after hrec
TNF alpha first immunotherapeutic cycle . -
pubmed
The results of this study showed the decreasing influence of hrec
TNF alpha administration on
cortisol secretion by adrenals with no disturbances in that circadian rhythm . -
pubmed
IN CONCLUSION : It might be possible that some side effects of hrec
TNF alpha administration are connected with the decreasing
cortisol level . -
pubmed
Anti -
lactoferrin autoantibodies : relation between epitopes and
iron - binding domain . -
pubmed
We hypothesized that the interaction of
anti - LF antibodies with the
LF iron binding domain might contribute to pathogenesis by inhibiting
iron chelation after neutrophil activation , thereby providing increased
iron availability for endothelial cell damage . -
pubmed
Therefore ,
anti - LF autoantibodies did not appear to modulate LF
iron - binding activity . -
pubmed
Total and
selenium - dependent
glutathione peroxidase activities in heart were unaffected by any treatment . -
pubmed
DHEA increased
catalase activity at
rest . -
pubmed
Exercise increased
catalase activity ( 71.5 + /- 7.9 vs. 97.4 + /- 9.5 mu mol x min-1 x mg protein-1 ) ; however , when
VitE was given , the response to exercise was attenuated ( 74.1 + /- 8.4 vs. 80.9 + /- 9.9 mu mol center dot min-1 x mg protein-1 ) . -
pubmed
The results show that neither mutation is likely to be the etiology for predisposition to a change of
high - density lipoprotein cholesterol and/or variation in lipid and lipoprotein levels , or for the occurrence of
coronary heart disease in Japanese populations . -
pubmed
Vav , a
GDP - GTP exchange factor for
Rho - family proteins , is
tyrosine phosphorylated following
TCR stimulation , suggesting that it may transduce
TCR signals . -
pubmed
Vav may influence the efficiency of
TCR - induced selection events by regulating the intracellular
calcium flux of thymocytes . -
pubmed
We demonstrated that the formation of
prostaglandin E2 (
PGE2 ) is highly dependent on the expression of
PGHS-2 enzyme in these cells . -
pubmed
TNF - alpha - induced
PGE2 production was evident after 12 h and was associated with a significant
TNF - alpha - mediated increase in
PGHS-2 immunoreactive protein . -
pubmed
A specific
PGHS-2 inhibitor ,
NS-398 , completely abolished the
TNF - alpha - mediated increase in
PGE2 production , suggesting that the
PGE2 formed in response to
TNF - alpha was derived from
PGHS-2 . -
pubmed
TNF - alpha - mediated PGHS-2 mRNA accumulation was observed at 1 h , remained elevated for 24 h , and was blocked by
actinomycin D , indicating that
TNF - alpha increases
PGHS-2 gene transcription . -
pubmed
All the
strains were characterized by the low production of higher
alcohols and the high production of
ethyl acetate , whereas consistent differences in the production of
acetaldehyde ,
acetoin and
acetic acid served to differentiate
star fruit apiculate
strains into six different phenotypes , present at different stages of the fermentation process . -
pubmed
Potent , non -
thiol inhibitors of
farnesyltransferase . -
pubmed
The structure - activity relationship of a series of non -
thiol CaaX analogs , which are inhibitors of
farnesyltransferase , is described . -
pubmed
These inhibitors contain a substituted
phenyl group at the N terminus , which may occupy a novel binding domain on the
Ras protein . -
pubmed
CD44 is a ubiquitous molecule also known as
hyaluronic acid or homing receptor . -
pubmed
These results indicate that the adhesion of RA synovial cells to matrices such as
hyaluronic acid through
CD44 could up - regulate
VCAM-1 expression and
VCAM-1 - mediated adhesion to T cells , which might in turn cause activation of T cells and synovial cells in
RA synovitis . -
pubmed
We found that
isoamyl alcohol induces cell extensions in
pkc1 deletion mutants but not in mutants with mutations in genes positioned downstream or upstream of the
PKC1 gene . -
pubmed
Cytochalasin D ( 0.08 mg / mL ) inhibited the formation of
actin cable but did not affect the induction of cell extensions . -
pubmed
At the end of the treatment period with
PGE1 ,
ET-1 plasma concentration decreased from 4.50+/-0.8 pmol / l to 3.6+/-1.1 pmol / l ( p = 0.002 ) , whereas it remained unchanged in placebo group . -
pubmed
A significant correlation between the decrease in
ET-1 plasma levels and the increase in the PFWD and MWD ( r = -0.92 , p & lt ; 0.0001 ; r = -0.78 , p = 0.002 , respectively ) was detected in
PGE1 treatment group . -
pubmed
Their stability constants are determined with a recent PC program SIRKO and the calculated values are : for
cobalt , log
beta 1 = 1.6 , log
beta 2 = 2.7 ; for
nickel , log
beta 1 = 1.8 , log
beta 2 = 3.0 ; and for
copper , log
beta 1 = 3.0 , log
beta 2 = 3.6 . -
pubmed
BACKGROUND AND
AIM : The aim of the study was to evaluate the effect on blood
glucose levels in non -
insulin - dependent
diabetics (
NIDDM ) of reduction of the
carbohydrate content through the use of a new , almost starch - free type of bread (
SF - bread ) . -
pubmed
Peak blood
glucose concentration was 14.8 + /- 2.3 mM on the control day and 11.6 + /- 1.7 mM on the
SF - bread day ( p & lt ; 0.001 ) . -
pubmed
In the second part of the study , the diet including
SF - bread reduced fasting blood
glucose from 13.3 + /- 3.5 mM to 10.2 + /- 2.0 mM ( p & lt ; 0.006 ) and the fraction of HbA1c from 0.090 + /- 0.014 to 0.081 + /- 0.015 ( p & lt ; 0.02 ) . -
pubmed
Serum
cholesterol levels were significantly reduced by the
SF - bread as compared to the ordinary diet ( 5.8 + /- 0.6 to 5.5 + /- 0.5 mM versus 5.7 + /- 0.8 to 5.8 + /- 0.7 mM ; p & lt ; 0.05 ) . -
pubmed
The beneficial reduction in mortality with
abciximab use in
diabetics classified as
insulin - requiring was from 8.1 % to 4.2 % , p = 0.073 . -
pubmed
Syntheses and evaluation of halogenated
cytisine derivatives and of bioisosteric
thiocytisine as potent and selective
nAChR ligands . -
pubmed
The
3 - halocytisines 7 possess subnanomolar affinities for ( alpha 4)(2)(beta 2)(3 )
nAChRs , higher than those found for
( -)- cytisine as well as for the
5 - halocytisines 8 and
3,5 - dihalocytisines 6 . -
pubmed
The most potent molecule was
3 - bromocytisine ( 7b ) with preferential selectivity ( 200 - fold ) for the ( alpha 4)(2)(beta 2)(3 ) subtype [ K(i)=10 pM (
alpha 4 beta 2 ) and 2.0 nM ( alpha 7 * ) ] . -
pubmed
Replacement of the
lactam with a
thiolactam pharmacophore to
thiocytisine ( 12 ) resulted in a subnanomolar affinity for the
( alpha 4 )( 2 )( beta 2 )( 3 ) nAChR subtype ( K(i)=0.832 nM ) , but in a drastic decrease of affinity for the
alpha 7 * subtype ;
thiocytisine ( 12 ) has a K(i ) value of 4000 nM ( alpha 7 * ) , giving a selectivity of 4800 - fold for the neuronal (
alpha 4 )( 2 )( beta 2 )( 3 )- nAChR and thus displaying the best affinity - selectivity profile in the series under consideration . -
pubmed
Four immunosuppressive
eras were analyzed : ( 1 ) the
precyclosporine era ,
era 1 ( n = 65 ; 16 % ) ; ( 2 ) the
cyclosporine era ,
era 2 ( n = 109 ; 27 % ) ; ( 3 ) the
tacrolimus era with monoclonal or polyclonal antibody induction therapy ,
era 3 ( n = 104 ; 26 % ) ; and ( 4 ) the
tacrolimus era with monoclonal and polyclonal antibody induction therapy ,
era 4 ( n = 128 ; 31 % ) . -
pubmed
Noradrenergic transmission may also be implicated because most psychostimulants increase
norepinephrine ( NE ) release , and numerous studies have indicated interactions between noradrenergic and dopaminergic neurons through
alpha1 - adrenergic receptors . -
pubmed
Similar to natural
enzyme , recombinant
xylitol dehydrogenase is a functional homotetramer with a stoichiometric content of catalytic
zinc in each 37 - kDa subunit . -
pubmed
Combined results of initial - rate analysis and isotope effect studies suggest that the
enzyme utilizes a preferentially ordered kinetic mechanism in which
NAD (+ ) binds before
D - sorbitol and
D - fructose is released before
NADH . -
pubmed
Stabilising mutations , which render
Aux / IAA proteins resistant to
auxin - mediated degradation , confer a wide range of phenotypes consistent with disruptions in
auxin response . -
pubmed
These results suggest a general model for
auxin signalling in which the modulation of the relative abundance of different
Aux / IAA proteins can determine which down - stream responses are induced . -
pubmed
Follow - up with regular
thyroglobulin assay and
( 131 ) I whole - body scan is capable of detecting residual or recurrent disease with great sensitivity and specificity . -
pubmed
This article will review the current management of differentiated
carcinoma of the thyroid and the possible causes of the reported inadequacy of
thyroglobulin and
( 131 ) I whole - body scan to detect residual or recurrent disease , and the increasing role of alternative imaging , particularly
( 18 ) F - FDG PET in the management of this curable
malignancy . -
pubmed
Her
prolactine and
estradiol levels were increased but
luteinizing hormone and
follicle - stimulating hormone were decreased . -
pubmed
The
H2S - induced relaxation of MAB was partially mediated by
ATP - sensitive K + ( KATP ) channel activity in vascular smooth muscle cells . -
pubmed
DL - propargylglycine ( a blocker of CSE ) at 10 microM abolished
L - cysteine - dependent increase in
H2S production and relaxation of
MAB . -
pubmed
The stimulation of KATP channels in vascular smooth muscle cells and
charybdotoxin /
apamin - sensitive
K + channels in vascular endothelium by
H2S represents important cellular mechanisms for
H2S effect on
MAB . -
pubmed
Using
[ ( 125 ) I]
alpha - bungarotoxin binding assays and voltage
clamp , we determined that the
nAChR expression , EC(50 ) , and Hill coefficient values for WT are 1.8 + /- 0.4 fmol , 30.3 + /- 1.1 microM , and 1.8 + /- 0.3 , respectively . -
pubmed
The periodicity in function ( EC(50 ) and macroscopic peak current ) and
nAChR expression reveals an average of 3.3 and 3.0
amino acids respectively , thus suggesting a helical secondary structure for the gammaTM4 . -
pubmed
This element is a key moiety of several enzymes in
iron containing
heme or nonheme form and transfer and storage protein ,
hemoglobin and
myoglobin . -
pubmed
The redox state of
iron is determined by
xanthine oxidase ,
cytochromes and
Hp or
ceruloplasmin and
ferroxidase activity of
apo - ferritin , respectively . -
pubmed
Some
vitamins ( C , B2- , B3- , B6- ,
B12 ) play also a role in the metabolism of
iron . -
pubmed
We found that , together , solvent - exposed
heme 1 ( having a large negative redox potential ) and
heme 2 ( having a large positive redox potential ) form a lock for electrons generated during the oxidation reaction The attachment of
HAO 's physiological electron transfer partner
cytochrome c(554 ) results in a positive shift in the redox potential of
heme 1 , and " opens the electron gate " . -
pubmed
The closeness of hemes 3 and 8 from different
enzyme subunits allows redistribution of the four electrons generated as a result of
hydroxylamine oxidation , among the three
enzyme subunits . -
pubmed
Toxaphene congener profiles were relatively invariant throughout the watershed and were dominated by
2,2,5 - endo,6 - exo,8,8,9,10 - octachlorobornane (
P-42a or
B8 - 806 ) with lesser amounts of several other
Cl ( 7 )- Cl ( 9 ) compounds , many of which remain unidentified . -
pubmed
B2Rs were coupled with
Gq / 11 and their activation resulted in
diacylglycerol and
inositol triphosphate release . -
pubmed
The mechanism for
prostaglandin - induced augmentation of
BK response is left open for future study . -
pubmed
Expression of
HOXB2 , a
retinoic acid signaling target in
pancreatic cancer and
pancreatic intraepithelial neoplasia . -
pubmed
RESULTS : We identified aberrant expression of several components of the
retinoic acid ( RA ) signaling pathway (
RARalpha ,
MUC4 ,
Id-1 ,
MMP9 ,
uPAR ,
HB - EGF ,
HOXB6 , and
HOXB2 ) , many of which are known to be aberrantly expressed in
pancreatic cancer and
PanIN . -
pubmed
NUCLEAR QUADRUPOLE AND
SPIN - ROTATION INTERACTION OF O IN
FORMALDEHYDE . -
pubmed
Phosphatidylinositol 3 - kinase - dependent activation of
akt , an essential signal for
hyperthermia - induced
heat - shock protein 72 , is attenuated in
streptozotocin - induced
diabetic heart . -
pubmed
We tested the hypothesis that
phosphatidylinositol 3 - kinase (
PI 3 - kinase)-dependent activation of
Akt is essential for the expression of
cardiac heat - shock protein 72 (
HSP72 ) and that this pathway is impaired in the
streptozotocin (
STZ)-induced
diabetic heart . -
pubmed
Compared with control heart ,
hyperthermia - induced
HSP72 expression and phosphorylation of
Akt were attenuated in the
STZ - vehicle heart . -
pubmed
Pretreatment with
wortmannin attenuated
hyperthermia - induced
HSP72 expression and phosphorylation of
Akt . -
pubmed
Wortmannin and
LY294002 inhibited
hyperthermia - induced
HSP72 expression and phosphorylation of
Akt . -
pubmed
Our results indicate that activation of
Akt , in a
PI 3 - kinase - dependent manner , is essential for
hyperthermia - induced
HSP72 expression in association with cardioprotection , suggesting impairment of this signaling pathway in the
STZ - induced
diabetic heart , probably due to
insulin deficiency . -
pubmed
Simultaneous quantitation of the
nucleotide analog
adefovir , its phosphorylated anabolites and
2 '- deoxyadenosine triphosphate by ion - pairing LC /
MS /
MS . -
pubmed
The study of
nucleoside / tide pharmacology has been hampered by difficulties encountered when trying to develop LC /
MS /
MS methods for these polar analytes . -
pubmed
In an attempt to identify a more convenient , selective and sensitive alternative to the analysis of the metabolism of radiolabeled parent
nucleotide traditionally used for in vitro cell culture studies , an LC /
MS /
MS method was developed for the quantitative detection of
adefovir and its phosphorylated metabolites in cellular samples . -
pubmed
Ion - pairing reversed phase LC using
tetrabutylammonium (
TBA ) and
ammonium phosphate had the best compromise between chromatographic separation and positive mode
MS /
MS detection . -
pubmed
A cross - validation showed comparable levels of
adefovir and its metabolites were determined using either LC /
MS /
MS or radioactivity detection . -
pubmed
fulgidus
FEN employing flap and double - flap substrates indicates that binding interactions with the 3'-extrahelical
nucleotide stabilise the ground state
FEN - DNA interaction , leading to stimulation of comparative reactions at DNA concentrations below saturation with the single flap substrate . -
pubmed
PATIENTS AND METHODS :
Tumors from the Cancer Research UK
Taxotere as Adjuvant Chemotherapy phase III trial were tested for
HER-2 using current diagnostic procedures . -
pubmed
Three six -
carbon and nine -
carbon aldehydes , natural plant volatiles produced by the plant
lipoxygenase pathway , were tested for their insecticidal activity against five species of stored - product beetles in feeding , fumigation and combined bioassays . -
pubmed
DHCR7 primarily catalyzes the reduction of
7 - dehydrocholesterol (
7DHC ) to
cholesterol . -
pubmed
Neither use of perianesthetic medications such as
labetalol ,
glycopyrrolate , or
remifentanil , or
ECT technical variables such as
seizure duration or electrode placement were associated with orthostatic blood pressure drop in the multivariate model . -
pubmed
Circulating oxidized LDL (
oxLDL ) levels are strongly correlated to
LDL -
cholesterol (
LDL - c ) and
apolipoprotein - B100 (
apoB100 ) , making it difficult to disentangle their independent contributions to cardiovascular risk . -
pubmed
Other major independent determinants of
oxLDL were sex ,
HDL -
cholesterol , and
LDL particle size . -
pubmed
After adjustment for age , sex ,
glucose tolerance status , and Framingham risk score , the
oxLDL /
apoB100 ratio was negatively related to
FMD ( P = 0.017 ) . -
pubmed
Carboxypeptidase Z (
CPZ ) links
thyroid hormone and Wnt signaling pathways in growth plate chondrocytes . -
pubmed
Carboxypeptidase Z (
CPZ ) removes
carboxyl - terminal basic
amino acid residues , particularly
arginine residues , from proteins . -
pubmed
CPZ contains a
cysteine - rich domain (
CRD ) similar to the
CRD found in the frizzled family of Wnt receptors . -
pubmed
We have previously shown that
thyroid hormone regulates terminal differentiation of growth plate chondrocytes through activation of
Wnt-4 expression and Wnt /
beta - catenin signaling . -
pubmed
The
Wnt-4 protein contains a C - terminal
arginine residue and binds to
CPZ through the
CRD . -
pubmed
The objective of this study was to determine whether
CPZ modulates Wnt /
beta - catenin signaling and terminal differentiation of growth plate chondrocytes . -
pubmed
Our results show that
CPZ and
Wnt-4 mRNA are co - expressed throughout growth plate cartilage . -
pubmed
Knockdown of either
Wnt-4 or
CPZ mRNA levels using an RNA interference technique or blocking
CPZ enzymatic activity with the carboxypeptidase inhibitor
GEMSA reduces the
thyroid hormone effect on both
alkaline phosphatase activity and
Col10a1 mRNA expression . -
pubmed
Overexpression of
CPZ in growth plate chondrocytes also removes the C - terminal
arginine residue from a synthetic peptide consisting of the
carboxyl - terminal 16
amino acids of the
Wnt-4 protein . -
pubmed
Removal of the C - terminal
arginine residue of
Wnt-4 by site - directed mutagenesis enhances the positive effect of
Wnt-4 on terminal differentiation . -
pubmed
These data indicate that
thyroid hormone may regulate terminal differentiation of growth plate chondrocytes in part by modulating Wnt signaling pathways through the induction of
CPZ and subsequent
CPZ - enhanced activation of
Wnt-4 . -
pubmed
PURPOSE : To determine the feasibility of combined long - term
luteinizing hormone - releasing hormone agonist - based
androgen suppressive therapy ( AST ) and dose escalation with high - dose - rate ( HDR ) brachytherapy for high - risk ( HRPC ) or very - high - risk
prostate cancer ( VHRPC ) . -
pubmed
Biophysical characteristics of the factor - bound nucleosomal arrays were determined by analytical
agarose gel electrophoresis ( AAGE ) and single molecules were visualized by atomic force microscopy (
AFM ) . -
pubmed
Interestingly , the study of chemically modified
Hb for use as
oxygen therapeutics has allowed for some basic understanding of extracellular Hb
toxicity , particularly in the absence of functional clearance mechanisms and in circulatory antioxidant depleted states . -
pubmed
The induction , not the maintenance , of HFS - induced LTP is blocked by a
N - methyl - D - aspartate ( NMDA ) receptor antagonist ,
D-2 - amino-5 - phosphonopentanoic acid (
D - AP5 ) . -
pubmed
Both the induction and maintenance of LTP of
Abeta - fiber - evoked fEPSPs are inhibited by a ROS scavenger , either
N - tert - butyl - alpha - phenylnitrone or
4 - hydroxy-2,2,6,6 - tetramethylpiperidine - N - oxyl . -
pubmed
Ca ( 2 +)-modulated membrane
guanylate cyclase in the testes . -
pubmed
To date , the
calcium - regulated membrane
guanylate cyclase Rod Outer Segment Guanylate Cyclase type 1 (
ROS - GC1 ) transduction system in addition to photoreceptors is known to be expressed in three other types of neuronal cells : in the pinealocytes , mitral cells of the olfactory bulb and the gustatory epithelium of tongue . -
pubmed
In this presentation , the authors review the existing information on the localization and function of
guanylate cyclase with special emphasis on
Ca ( 2 +)-modulated membrane guanylate cyclase , ROS -
GC1 , in the testes . -
pubmed
The role of ROS -
GC1 and its
Ca ( 2 +)-sensing modulators in the processes of spermatogenesis and fertilization are discussed . -
pubmed
The inhibition of the
SHH signaling pathway by the specific inhibitor
cyclopamine abolished
CRCC cell growth as assessed by cell counting ,
BrdU incorporation studies , fluorescence - activated cell sorting and beta - galactosidase staining . -
pubmed
[ Effects of
adiponectin gene SNP45T / G on changes of serum lipid ratios induced by high -
carbohydrate / low - fat diet in healthy Chinese youth ] . -
pubmed
OBJECTIVE : To investigate the effects of
adiponectin gene (
APM1 ) SNP45T / G on serum lipid ratios and their responses to high -
carbohydrate / low - fat ( HC / LF ) diet in healthy young Chinese . -
pubmed
The
ras gene family provides a global effect on gene expression by encoding small
GTP - binding proteins which act as molecular switches connecting extracellular signals with nuclear transcription factors . -
pubmed
Late Onset
Central Hypoventilation Syndrome due to a Heterozygous
Polyalanine Repeat Expansion Mutation in the
PHOX2B Gene . -
pubmed
This report describes a 6 year old girl with late onset
central hypoventilation syndrome due to a heterozygous
polyalanine repeat expansion mutation in the
PHOX2B gene . -
pubmed
Mangiferin was not a substrate for the cytosolic
sulphotransferases . -
pubmed
The
ferric iron reducing ability of the glucuronidated reaction mixture was reduced , while the free radical scavenging abilities of
mangiferin , utilising on - line
post - column HPLC - DAD -
DPPH and HPLC - DAD -
ABTS + assays , were eliminated , providing further evidence that the
catechol arrangement at C-6 and C-7 was the preferred site of conjugation . -
pubmed
In contrast , expression of the
afs1 gene was strongly expressed by the above four
carbohydrates and less strongly induced by galactan . -
pubmed
( 1 ) H - NMR analysis revealed that
rAFS1 degraded
arabinofuranosyl side chains that were both singly and doubly linked to the backbones of arabinoxylan and
L - arabinan . -
pubmed
On the other hand ,
rAFQ1 preferentially released
arabinose linked to C-3 of single - substituted
xylose or
arabinose residues in the two polysaccharides . -
pubmed
Risk of
tacrolimus toxicity in
CYP3A5 nonexpressors treated with intravenous
nicardipine after kidney transplantation . -
pubmed
Compared with the other two groups combined ,
CYP3A5 * 3 /* 3 cases had higher median dose - adjusted MaxC0 ( 330 vs. 175 , P=0.012 ) , less time to MaxC0 ( 42 vs. 72 hr , P<0.001 ) , and more scheduled
tacrolimus doses held per patient ( 1.75 vs. 0.4 , P=0.007 ) . -
pubmed
CONCLUSION :
CYP3A5 nonexpressors simultaneously treated with
tacrolimus and
CIVN may be at increased risk for
tacrolimus toxicity . -
pubmed
The present study examined the effects of
4 - phenylbutyric acid (
4 - PBA ) , an
ER chaperone , on acini and UPR components . -
pubmed
Effects of
4 - PBA were measured on secretion ,
calcium , and
trypsin activation . -
pubmed
RESULTS :
4 - Phenylbutyric acid led to an increase of secretion , whereas
trypsin activation with supraphysiological
cholecystokinin was significantly reduced . -
pubmed
4 - Phenylbutyric acid prevented
chaperone - binding protein up - regulation , diminished
protein kinaselike ER kinase , and
c - Jun NH2 - terminal kinase phosphorylation , prohibited
X - box - binding protein 1 splicing and
CCAAT / enhancer - binding protein homologous protein expression ,
caspase 3 activation , and apoptosis caused by supraphysiological
cholecystokinin . -
pubmed
CONCLUSION : By incubation with
4 - PBA , beneficial in
urea cycle deficiency , it was possible to enhance enzyme secretion to suppress
trypsin activation , UPR activation , and proapoptotic pathways . -
pubmed
A conserved motif in the
ITK PH - domain is required for
phosphoinositide binding and
TCR signaling but dispensable for adaptor protein interactions . -
pubmed
Binding of the membrane phospholipid
phosphatidylinositol 3,4,5 - trisphosphate (
PIP ( 3 ) ) to the Pleckstrin Homology ( PH ) domain of the
Tec family protein tyrosine kinase ,
Inducible T cell Kinase (
ITK ) , is critical for the recruitment of the
kinase to the plasma membrane and its co - localization with the
TCR - CD3 molecular complex . -
pubmed
However , the biological significance of the FYF
amino acid motif in the
ITK - PH domain is unknown . -
pubmed
Here we identify the
SWI / SNF
ATP - dependent nucleosome - remodeling complex as a component of the RNA - mediated transcriptional silencing pathway . -
pubmed
Induced pluripotent stem cells mediate the release of
interferon gamma - induced protein 10 and alleviate
bleomycin - induced
lung inflammation and
fibrosis . -
pubmed
Administration of either 3 - gene iPSCs or iPSC - CM significantly attenuated
collagen content and
myeloperoxidase activity , diminished neutrophil accumulation , and rescued
pulmonary function and recipient survival after
bleomycin treatment . -
pubmed
Notably , both treatments reduced the levels of inflammatory
cytokines and
chemokines , including
interleukin 1 (
IL-1 ) ,
IL-2 ,
IL-10 ,
tumor necrosis factor - alpha , and
monocyte chemotactic protein 1 yet increased the production of the antifibrotic
chemokine interferon - gamma - induced protein 10 (
IP-10 ) in
bleomycin - injured lungs . -
pubmed
IR - induced overexpression of
UCP2 was suppressed by
ramiprilat and
losartan . -
pubmed
CONCLUSION : These findings suggest that
losartan and
ramiprilat can suppress
UCP2 expression following myocardial IR , and by this mechanism may protect the myocardium against
IR injury . -
pubmed
Single - layer
magnesium fluoride (
MgF ( 2 ) ) and antireflective (
AR ) coating at 193 nm were fabricated on a convex conical substrate holder ( with diameter 225 mm , apex angle 140
deg , and height 41 mm ) by thermal evaporation . -
pubmed
From the reflectance spectra , uniform optical performance was also obtained for the 193 nm
AR coating deposited on fused -
silica substrates . -
pubmed
From the beginning of treatment to the
CT and at d8 , d15 and d30 , oral
morphine equivalent dose ( OMED ) did not significantly differ . -
pubmed
BACKGROUND : Developing a round - the -
clock artificial pancreas requires accurate and stable continuous
glucose monitoring . -
pubmed
The radiochemical yield of the
[ 1 -( 11 ) C ] Tpi - containing RGD at the end of synthesis (
EOS ) was 5.9 + - 1.9 % ( n = 4 ) , for a total synthesis time of about 35 min . -
pubmed
While the
PAPS - bound
enzyme spends the majority ( 95 % ) of its time in the constricted state , the pore isomerizes between the open and closed states when the
nucleotide (
PAPS ) is bound . -
pubmed
Creatine kinase (
CK ) ,
aspartate aminotransferase (
AST ) ,
alanine aminotransferase (
ALT ) ,
gamma - glutamyl transpeptidase (
GGT ) ,
lactate dehydrogenase (
LDH ) ,
alkaline phosphatase (
AP ) ,
cortisol and
C - reactive protein (
CRP ) ,
glutathione peroxidase (
GPx ) and
reductase activities (
GR ) and
C - reactive protein (
CRP ) levels , total antioxidant status , and
cortisol levels were measured in serum the day before and immediately after the race . -
pubmed
CK and
LDH and
cortisol levels were negatively correlated to total energy , protein , and fat intake . -
pubmed
Intake of
vitamin A , B1 , B2 , B6 and
niacin was negatively correlated to
LDH and AP . -
pubmed
A negative correlation was also found between
CK activity and
Na ,
Fe , and
Zn intake . -
pubmed
A positive correlation was found between serum
GPx and intake of energy ,
carbohydrates , proteins , A and
B vitamins , and
folic acid . -
pubmed
Plasma proinflammatory
cytokines and urinary
F2 - isoprostanes were measured . -
pubmed
Finally , suppression of
PTPalpha resulted in increased phosphorylation of
focal adhesion kinase on
Tyr-407 , which induced the recruitment of
vinculin and the formation of a novel
focal adhesion kinase complex in response to
ErbB2 activation in mammary epithelial cells . -
pubmed
The
alpha - fetoprotein fraction
L3 (
AFP - L3 ) , which is synthesized by malignant cells and incorporates a
fucosylated oligosaccharide , has been investigated as a diagnostic and prognostic marker for
hepatocellular carcinoma (
HCC ) . -
pubmed
ATG4 processes
ATG8 precursor to expose its C - terminal
glycine for
phosphatidyl ethanolamine ( PE ) lipidation . -
pubmed
GFP fusion proteins of
ATG8s , especially of
ATG8s with innate C - terminal - exposed
glycines , localized to punctate autophagic membranes . -
pubmed
Among numerous
fructofuranose conformers discovered ( about 250 ) , a pair of the
( ( 3 ) T2 ) alpha - and (
E3 ) beta - anomers are energetically most preferred and lie at least 12 kJ / mol above the global minimum . -
pubmed
The commonly used M06 - 2X density functional performs well compared to
MP2 and
G4 theory at identifying the low - energy
fructose minima , including the global one , and at reproducing their intramolecular H - bond geometric parameters . -
pubmed
The purified
IgG , unlike native sera , mirrored
IVIg reactivity to a wide array of
HLA - I /- II alleles , indicating that
anti - HLA IgG may be masked in normal sera - either by
peptides derived from soluble
HLA or by those from antibodies . -
pubmed
Comparison of
HLA types with the profile of
HLA antibodies showed
auto - HLA IgG to the donors '
HLA antigens in this order of frequency :
DPA ( 80 % ) ,
DQA ( 71 % ) ,
DRB345 ( 67 % ) ,
DQB ( 57 % ) ,
Cw ( 50 % ) ,
DBP ( 43 % ) ,
DRB1 ( 21 % ) , A ( 14 % ) and B ( 7 % ) . -
pubmed
At 1 h
post feeding ,
L - Arg mares had lower ( P<0.05 ) plasma concentrations of
histidine ,
glutamic acid ,
proline ,
isoleucine ,
threonine ,
phenylalanine ,
leucine ,
valine ,
alanine , and
taurine . -
pubmed
The mutant
Rht - B1c displayed lower and slower
electrolyte leakage in comparison with the wild - type
Rht - B1a . -
pubmed
It is shown that when the growth substrate temperature is sufficiently high , a portion of a
Ag NP can be melted for serving as the catalyst to precipitate
GaZnO on the residual
Ag NP and form a
GaZnO NN . -
pubmed
Such superior field emission performances are attributed to a few factors , including ( 1 ) the low work function and high conductivity of the grown
GaZnO NNs due to highly degenerate
Ga doping , ( 2 ) the sharp - pointed geometry of the vertically aligned
GaZnO NNs , ( 3 ) the
Ag doping in VLS precipitation of
GaZnO for further reducing
NN resistivity , and ( 4 ) the residual small
Ag NP at the
NN tip for making the tip even sharper and tip conductivity even higher . -
pubmed
Design , synthesis , in - vitro antiproliferative activity and
kinase profile of new
picolinamide based
2 - amido and ureido quinoline derivatives . -
pubmed
Isotretinoin Laboratory Test Monitoring -- A Call to Decrease Testing in an
Era of High - Value , Cost - Conscious Care . -
pubmed
Furthermore , we uncovered
Dfd as a potential regulator of synaptic specificity , as it represses expression of the synaptic cell adhesion
molecule Connectin (
Con ) . -
pubmed
Two died while receiving high - dose systemic
corticosteroids ,
methotrexate , and after recent exposure to
anti - tumor necrosis factor - alpha biological DMARDs , and 2 during hematopoietic stem cell transplantation procedure . -
pubmed
Infliximab and
adalimumab are
anti - TNF - alpha antibodies that specifically block the interaction of
TNF - alpha with its receptors . -
pubmed
Vitamin A Transport and Cell Signaling by the
Retinol - Binding Protein Receptor STRA6 . -
pubmed
Vitamin A ,
retinol , circulates in blood bound to
retinol binding protein (
RBP ) . -
pubmed
In some tissues , the
retinol - RBP complex (
holo - RBP ) is recognized by a membrane receptor , termed
STRA6 , which mediates uptake of
retinol into cells . -
pubmed
Recent studies have revealed that , in addition to serving as a
retinol transporter ,
STRA6 is a ligand - activated cell surface signaling receptor that , upon binding of
holo - RBP activates
JAK /
STAT signaling , culminating in the induction of
STAT target genes . -
pubmed
It has further been shown that
retinol transport and cell signaling by
STRA6 are critically interdependent and that both are coupled to intracellular
vitamin A metabolism . -
pubmed
The formation of
BPA intermediates was investigated in detail by using high - performance liquid chromatography - mass spectrometry ( HPLC /
MS ) . -
pubmed
A covariance analysis was conducted to analyze differences of oxygenated
hemoglobin (
oxy - Hb ) changes in prefrontal cortex ( PFC ) between the two groups and reduce the influence of the severity of
depression . -
pubmed
As a result of this study , we have identified ProxyPhos library members that are most suitable for the detection of proximally phosphorylated peptides ,
PPi ,
UTP , and a DpYD peptide motif , and have generally provided a foundation for the selection of
ProxyPhos chemosensors for further development of specific biologically relevant assays . -
pubmed
Expression of
PLA2 was significantly reduced following treatment with
28 - homobrassinolide . -
pubmed
In general ,
flavanols and
phenolic acids exhibited their maximum concentration at week 5 and then decreasing significantly during the
rest of maturation . -
pubmed
Focal dose escalation for
prostate cancer using
68Ga - HBED - CC PSMA PET /
CT and MRI : a planning study based on histology reference . -
pubmed
The aim of this study was to demonstrate the feasibility and to evaluate the
tumour control probability ( TCP ) and normal tissue complication probability (
NTCP ) of radiation therapy dose painting using
68Ga - HBED - CC PSMA PET /
CT , mpMRI or the combination of both in primary
PCa . -
pubmed
Typical management of
VWD patients is also discussed , including the use of
desmopressin and
VWF concentrates . -
pubmed
Src is a
tyrosine kinase known to both phosphorylate
Cx43 ( residues Y247 and Y265 ) and affect gap junction intercellular communication . -
pubmed
However , the
Cx43 carboxyl - terminal (
CT ) domain contains additional
tyrosine residues and proteomic discovery mass spectrometry data identified Y313 as a potential phosphorylation target . -
pubmed
, which still observed
tyrosine phosphorylation by
Src when using a
Cx43 Y247 / Y265F mutant , we addressed the possibility of Y313 phosphorylation ( pY313 ) by
Src . -
pubmed
When
v - Src was over - expressed ,
Cx43 WT localized intracellularly , while all of the single and double mutants remained able to form plaques and transfer
dye , albeit variable in number and amount , respectively . -
pubmed
In addition to conventional diagnostics , the measurement of enhanced
tumor cell metabolism using
F-18 fluorodeoxyglucose (
18F - FDG ) for increased glycolysis or
C-11 acetate for
fatty acid synthesis by positron emission tomography / computed tomography ( PET /
CT ) is well established for clinical management of
HCC . -
pubmed
Objective : A putative relationship between markers for the
serotonin system and the personality scale self - transcendence ( ST ) and its subscale spiritual acceptance (
SA ) has been demonstrated in a previous PET study of
5 - HT1A receptor binding in healthy control subjects . -
pubmed
NBT treatment decreased the expression of a
neuroblastoma - derived
MYC (
MYCN ) and
multidrug resistance - associated protein 1 (
MRP1 ) as well as downregulating
Akt ,
GSK3beta , and
beta - catenin . -
pubmed
Through subsequent sequence analysis and thermal shift assays , it was found that the
purine /
pyrimidine content modulates the overall stability of the
polymerase complex , resulting in alteration of the activity of viral RNA synthesis . -
pubmed
Appearances of
pigment changes and
hemorrhages were some of the differences found when compared to the conventional
CFP . -
pubmed
Consistent with this , stimulation of endothelial cells ( EC ) with
thrombin caused an increase in
BiP /
GRP78 levels and inhibition of
ER stress with
4 - phenylbutyric acid (
4 - PBA ) prevented this response as well as increase in
VCAM-1 ,
ICAM-1 ,
IL-6 , and
IL-8 levels . -
pubmed
Importantly ,
thrombin - induced
Ca2 + signaling and EC permeability were also prevented upon
BiP /
GRP78 inactivation . -
pubmed
The aim of this study was to optimize a multistep
Triton X-100 based protocol ( either using
Triton X-100 /
ammonium hydroxide mixture alone or after its modification with
DNase ,
sodium dodecyl sulfate or
trypsin ) that could achieve maximum decellularization with minimal liver ECM destruction suitable for subsequent organ implantation without immune rejection . -
pubmed
Among all
Triton X-100 modified protocols , a
Triton X-100 /
DNase - based one was considered the most suitable . -
pubmed
The twin -
arginine translocation (
Tat ) system transports folded proteins across membranes of prokaryotes , plant plastids , and some mitochondria . -
pubmed
This complex was initially found when the photo cross - linking
amino acid p - benzoyl - l - phenylalanine (
Bpa ) was introduced at position I50 on the periplasmic side of the first trans - membrane domain of
TatC. -
pubmed
Lactate dosage was performed at ED - HDU admission ( T0 ) , after 2 h (
T2 ) , 6 h (
T6 ) and 24 h ( T24 ) ;
lactate clearance was calculated at
T2 and
T6 [
T2 : ( ( LAC T0 - LAC
T2 / LAC T0)*100 ) ] ;
T6 : [ ( LAC T0 - LAC
T6 / LAC T0)*100 ] . -
pubmed
The
G protein - coupled estrogen receptor (
GPER ) is a seven - transmembrane - domain receptor that mediates non - genomic
estrogen related signaling . -
pubmed
Therefore , a better understanding of the role
GPER plays in
cancer biology may lead to the identification of novel therapeutic targets , especially among
estrogen - related
cancers . -
pubmed
Median progression - free survival ( PFS ) for the first remission was 105 days ; lack of expression of
CD3 on flow cytometry ( P < .0001 ) and pretreatment with
steroid ( P = .012 ) were significantly associated with shorter PFS . -
pubmed
An experimental design with four factors :
enzyme / substrate ratio , quantity of
xylose , hydrolysis and cooking times was used to create a range of enzymatic hydrolysates . -
pubmed
DNA sequencing for the beta -
globin gene revealed rare a -90 ( C > T ) (
HBB : c.-140 C > T ) mutation for the woman . -
pubmed
We also developed an immunohistochemical stain for
EFEMP1 applicable to
formalin - fixed tissue sections and performed electron microscopy in one case . -
pubmed
Although the
EFEMP1 deposits were weakly to moderately Congo red - positive with absent to weak birefringence , they were strongly positive for
EFEMP1 by immunohistochemistry , had the characteristic fibrillar ultrastructure of
amyloid , and were readily identified by mass spectrometry . -
pubmed
Because
EFEMP1 amyloid is only weakly Congo red positive it may be overlooked without a high index of suspicion . -
pubmed
Based on its distinctive features , we propose that
EFEMP1 amyloidosis be considered a new
amyloid type . -
pubmed
AIM : Treatment with
obeticholic acid (
OCA ) affects the blood lipid profile . -
pubmed
Six ( 6 ) articles with 10 trials for
low - density lipoprotein cholesterol (
LDL - C ) , and 8 trials for
high - density lipoprotein cholesterol (
HDL - C ) , total
cholesterol ( TC ) , and
triglycerides ( TG ) levels were included in the meta - analysis . -
pubmed
The pooled results showed that the levels of TC ( WMD : 6.357 mg / dl ) and
LDL - C ( WMD : 6.067 mg / dl ) increased while
TG ( WMD : -22.417 mg / dl ) decreased after treatment with
OCA . -
pubmed
METHODS : We use patch
clamp electrophysiology in acute cerebellar slices and immunostaining to identify the specific ion channels targeted by
chlorzoxazone -
baclofen . -
pubmed
ILA ( 1 - 10 mM ) significantly attenuated lipopolysaccharide ( LPS)-induced activation of
NF - kB in macrophages . -
pubmed
ILA significantly attenuated
TNF - alpha- and LPS - induced increase in the pro - inflammatory
cytokine IL-8 in intestinal epithelial cells . -
pubmed
ILA increased mRNA expression of the
aryl hydrogen receptor (
AhR)-target gene
CYP1A1 and
nuclear factor erythroid 2 - related factor 2 (
Nrf2)-targeted genes
glutathione reductase 2 (
GPX2 ) ,
superoxide dismutase 2 (
SOD2 ) , and
NAD ( P ) H dehydrogenase (
NQO1 ) . -
pubmed
In addition , we assessed whether there were any associations between an increase in
oxygen requirement and known risk factors for
critical illness in COVID-19 , including elevation of liver enzymes and
C - reactive protein (
CRP ) ,
lymphocytopenia , high D - dimer levels and the chest computed tomography ( CT ) score . -
pubmed
Comorbid
cardiovascular disease ,
lymphocytopenia , elevated
CRP , liver enzyme and D - dimer levels , and higher chest CT score were significantly associated with an increase in
oxygen requirement CONCLUSIONS : The expanded CURB-65 score can be a better predictor of an increase in
oxygen requirement in patients with
SARS - CoV-2 pneumonia . -
pubmed
Synthetic thick filaments : A new avenue for better understanding the
myosin super - relaxed state in healthy , disease , and
mavacamten - treated cardiac systems . -
pubmed
Using these STFs , we show several pathophysiological insults in
hypertrophic cardiomyopathy , including the R403Q
myosin mutation , phosphorylation of
myosin light chains , and increased
ADP :
ATP ratio destabilize the
SRX population . -
pubmed
Furthermore ,
wild - type myosin containing
STFs , but not
S1 ,
HMM , or STFs - containing R403Q
myosin , recapitulated the
ADP - induced destabilization of the
SRX state . -
pubmed
Studies involving a clinical - stage small molecule inhibitor ,
mavacamten , showed that it is not only more effective in increasing
myosin SRX population in STFs than in
S1 or HMM , but it also increases
myosin SRX population equally well in STFs made of healthy and disease - causing R403Q
myosin . -
pubmed
Importantly , we also found that pathophysiological perturbations such as elevated
ADP concentration weakens the
mavacamten ' s ability to increase the
myosin SRX population , suggesting that
mavacamten - bound
myosin heads are not permanently protected in the
SRX state but can be recruited into action . -
pubmed
Antipsychotic potential of the
type 1 cannabinoid receptor positive allosteric modulator
GAT211 : preclinical in vitro and in vivo studies . -
pubmed
In this study , we compared the activity and stability of in situ or crosslink - immobilized
enzymes in a
CaCO3 biomineral carrier . -
pubmed
More than 30 % of the initial
enzyme activity was preserved for both the systems after 180 days upon 15 activity measurements at room temperature , confirming the improved stability of these
enzyme systems ( 100 mM
phosphate buffer , pH 8.0 ) ; however , differences in
enzyme loading , activity , and characteristics were observed for each of these methods . -
pubmed
Based on the same amount of immobilized
enzyme ( 0.2 mg ) , the specific activities of hydrolysis of
p - nitrophenyl butyrate substrate at room temperature of in situ immobilized
carboxyl esterase ( CE ) and crosslinked CE were 11.37 and 7.63 mM min-1 mg-1 , respectively ( 100 mM
phosphate buffer , pH 8.0 ) . -
pubmed
Moreover , based on the kinetic behavior , in situ immobilized CE exhibited improved catalytic efficiency ( Vmax Km-1 ) of the
enzyme , exhibiting 4 - fold higher activity and efficiency values than those of the CE immobilized in
CaCO3 . -
pubmed
This is the first study to describe the stabilization of
enzymes in
CaCO3 and compare the
enzyme kinetics and efficiencies between in situ immobilization and crosslinking in
CaCO3 carriers . -
pubmed
In the case of
PHA , increased
neoAg expression was correlated with an increased incorporation of
14C - leucine into
C5 , C6 , C7 , and C8 antigens , which was detected by immunodiffusion and autoradiography . -
pubmed
That
naloxone alters the depth of inhalational anesthesia suggests that anesthetics may release an endogenous
morphine - like factor (
MLF ) in the central nervous system . -
pubmed
Intracellular
Ca2 + concentration ( [
Ca2+]i ) was raised by photolysis of a caged
calcium compound ,
nitr-5 , and its effects on the mechano - electrical transduction (
MET ) current were studied by a whole - cell patch electrode voltage
clamp technique in dissociated hair cells of a chick . -
pubmed
Nitr-5 was loaded into the hair cell by incubation with the membrane - permeable form of the compound ( nitr-5
AM ) . -
pubmed
The extent of
MET current decay was reduced or disappeared in 20 - 100 microM
Ca2 + extracellular media and the offset time course was prolonged at the membrane potential of -50 mV. -
pubmed
These results ( paragraphs 3 - 6 ) suggest that the
MET current adaptation is accelerated by the increase of [
Ca2+]i , and that
Ca2 + ions entering through
MET channels are essential in the development of adaptation . -
pubmed
The adaptation of the
MET current was reversibly reduced in a
dihydrostreptomycin (
DHSM , 20 - 50 microM ) medium . -
pubmed
These delays in the development and the subsidence of adaptation suggest a presence of a
Ca2 + buffer site intracellularly between the adaptative site and the
MET channel . -
pubmed
Cyclophosphamide reduced the
arthritis , together with decreases in the lymphocyte count and the serum
IgG level . -
pubmed
Octreotide given before the induction of
pancreatitis significantly reduced the levels of
serum amylase and
lipase ,
ascites amylase concentration , degree of leukocyte infiltration , and focal areas of pancreatic tissue
necrosis . -
pubmed
Using
ciprofloxacin concentrations adjusted for a higher MIC obtained in pH - adjusted urine and
MHB , PAE values were similar for urine and
MHB ( approximately 280 min at 40 x MIC ) . -
pubmed
Selenium had no effect on
5 - aminolevulinic acid synthetic ability but inhibited
5 - aminolevulinic acid dehydratase and
PBG deaminase activities . -
pubmed
The results suggest the possible regulatory role of
selenium on
chlorophyll synthesis by interacting with
sulfhydryl containing enzymes
5 - aminolevulinic acid dehydratase and
porphobilinogen deaminase . -
pubmed
The effects of
cisplatin on electrical and mechanotransducer properties of the crayfish stretch receptor neuron was studied using a microelectrode voltage
clamp technique . -
pubmed
The need for extracellular
calcium ,
calcium influx , and specifically for
calcium influx through the
calcium channel was examined . -
pubmed
Nifedipine , a
dihydropyridine calcium channel antagonist , significantly ( P less than 0.01 ) reduced
angiotensin II- and
potassium - stimulated
aldosterone secretion , but had no effect on
dbcAMP - stimulated
aldosterone secretion ( 100 + /- 14 vs 105 + /- 19 pmol/10(6 ) cells ) . -
pubmed
Angiotension II and
potassium activation of both the early
aldosterone biosynthetic pathway ( as reflected by
pregnenolone production in the presence of
cyanoketone ) and also its late pathway ( as reflected by the conversion of exogenous
corticosterone to
aldosterone in the presence of
cyanoketone ) were significantly ( P less than 0.01 ) inhibited by
lanthanum ,
nifedipine and by reducing the extracellular
calcium concentration . -
pubmed
These observations suggest that : the extracellular
calcium dependency of
dbcAMP- ,
angiotensin II- and
potassium - stimulated
aldosterone secretion reflects a need for
calcium influx ; with
dbcAMP stimulation , activation of the late pathway is dependent on
calcium influx by a
calcium channel - independent mechanism , whereas activation of the early pathway is not dependent on extracellular
calcium or
calcium influx and activation of both the early and late pathway by
angiotensin II and
potassium is dependent on
calcium influx by a
calcium channel - dependent mechanism . -
pubmed
The specimens were fixed in
glyceraldehyde , washed in a cacodylate buffer ,
post - fixed in
potassium ferrocyanide reduced - osmium tetroxide , stained with uranyl acetate and included in EPON 812 . -
pubmed
In frontal cortex , the affinity of
opiate receptors for
dihydromorphine was significantly reduced in brains of
alcoholics . -
pubmed
[ Experiences with
L - carnitine in the
post - stress phase ] . -
pubmed
Beside
carnitine and
acetylcarnitine levels in plasma and urine the following parameters were determinated to evaluate the effect of
carnitine : for the metabolism of
fatty acids :
triglycerides , free
fatty acids (
FFA ) ,
alpha - hydroxy - butyrate for the metabolism of
carbohydrates :
glucose ,
insulin and
lactate in plasma . -
pubmed
Finally for
amino acid metabolism :
urea ,
creatinine ,
cholinesterase and kolloid osmotic pressure in plasma as well as
ureanitrogen and
alpha - aminonitrogen excretion in urine . -
pubmed
Thus , while
P-450PCN - E may be oligomeric in microsomal membranes , target inactivation analysis does not appear to give conclusive results regarding the states of aggregation of these microsomal
proteins . -
pubmed
Analysis of the structure - activity relationships indicated that , for maximum biological activity ,
steroids must have a planar nucleus ( as in
delta 4 and 5 alpha - reduced
steroids ) , a
hydroxyl or
keto group at position 3 , and
hydroxyl groups at positions 17 and 20 beta . -
pubmed
Previous studies have shown that the central nervous system is able to convert
testosterone into
17 - beta - hydroxy-5 - alpha - androstan-3 - one (
DHT ) , by the action of the enzyme
5 - alpha - reductase . -
pubmed
Ki values calculated for
taurocholate ( 43 microM ) and
testosterone glucuronide ( 28 microM ) indicated that these two inhibitors were relatively weak competitors for this
E2 17 G transport site . -
pubmed
A dose - related decrease in ciliary beating frequency (
CBF ) was seen after perfusion with both
BDP and
flunisolide as well as
propylene glycol . -
pubmed
The decrease in
CBF following perfusion with
propylene glycol was partially reversible upon re - perfusion with medium alone , whereas the decrease seen after
BDP and
flunisolide was irreversible . -
pubmed
Amiodarone has been found to decrease serum T3 by blocking peripheral
T4 5 '- deiodinase . -
pubmed
The value for
MPF was determined by the radioactive microsphere method and PS was assessed by an integral tissue - uptake method using
[ 51Cr ]- EDTA as test molecule . -
pubmed
During coronary vasodilation achieved by intra - coronary infusion of
adenosine ,
MPF increased markedly from 101.5 + /- 14.2 to 778.1 + /- 135.2 ml X min-1 X 100 g-1 in subendocardial layers and from 77.7 + /- 10.3 to 635.3 + /- 85.9 ml X min-1 X 100 g-1 in subepicardial layers of the left ventricular wall . -
pubmed
Raising
MPF by 800 % was accompanied by a modest but statistically significant increase in
PS [ 51Cr ]- EDTA . -
pubmed
The effects of
chlorpromazine ,
haloperidol , and
thioridazine on brain
noradrenaline content elevated by previous administration of
MAO inhibitors . -
pubmed
They are
prostaglandin synthetase inhibitors , three to ten times less active as
indomethacin . -
pubmed
Within 15 min of
nicorandil administration , heart rate increased and peripheral resistance decreased ( dose - dependent ) ; the preejection period ( corrected ; PEPc ) and the ratio of
PEP to left ventricular ejection time ( LVET ) -
PEP / LVET - were shortened by the 60 mg dose ; the heart rate corrected electromechanical systole ( QS2c ) was almost unchanged ; left ventricular end diastolic and systolic diameters ( EDD and
ESD ) were diminished . -
pubmed
The effects on certain parameters had not completely disappeared after 6 h.
ISDN produced a similar pattern with somewhat less intense effects ; although PEPc and
PEP / LVET were prolonged . -
pubmed
The predominant action of
ISDN was to reduce preload and thereby to lengthen PEPc and
PEP / LVET ; in addition , it lessened afterload . -
pubmed
Although
nicorandil caused a distinct reduction in preload , as shown by the smaller EDD , the effects of afterload reduction were predominant at the high dose induced shortening of PEPc and
PEP / LVET . -
pubmed
Dense deposits revealed by
electron microscopy were found in the regions where IF of
IgG and
C3 was observed . -
pubmed
Acid buffer eluates from liver and kidney contained
IgG reactive with nuclear
antigens . -
pubmed
The positive relationship between
Cl1MU and
Cl3MX in both smokers and nonsmokers ( r = 0.98 , P less than 0.001 ) supports the concept that two N - demethylation pathways for
theophylline metabolism are under common regulatory control and involve a form of
cytochrome P450 distinct from that mediating 8 - hydroxylation of
theophylline to
DMU . -
pubmed
These results suggest that cigarette smoking induces both of the
cytochrome P450 - mediated pathways of
theophylline metabolism but that N - demethylation may be increased to a greater extent than 8 - hydroxylation . -
pubmed
All the
neutral glycosphingolipids were isolated and their structure proved by the quantitative determination of their components , degradation by acid and specific
glycohydrolases and permethylation and gas - liquid chromatographic - mass spectrometric assay of the
methylated sugars . -
pubmed
In fibroblasts ,
growth factor stimulation results in the recruitment of
Grb2 -
mSos1 into complexes with activated receptors and cytoplasmic phosphoproteins such as
Shc , which are apparently involved in
Ras activation , and subsequently to an increase in
mSos1 phosphorylation on
serine and
threonine . -
pubmed
These common phosphorylation sites have been mapped to a region encompassing the first three
proline ( pro)-rich motifs in the tail of
mSos1 . -
pubmed
These findings suggest a potential inhibitory role for
MAP - kinase in attenuating
nucleotide exchange on
Ras , by uncoupling
mSos1 from membrane - bound receptor complexes that lead to
Ras activation . -
pubmed
The mechanism of action of
aspirin -- is there anything beyond
cyclo - oxygenase ? -
pubmed
Most workers agree that
aspirin inhibits the
cyclo - oxygenase enzyme in the platelets leading to reduced formation of
prostaglandin G2 , the precursor of
thromboxanes . -
pubmed
Competition radioimmunoassay ( CRIA ) revealed
Ab2 specificities towards Ab1 antigen binding sites by inhibition of
HBsAg /
Ab1 binding . -
pubmed
The
androgen receptor belongs to the family of nuclear receptors and contains three functional domains : a
carboxy - terminal hormone binding region , a central
cystein rich DNA binding region and an
amino - terminal region involved in the expression of
androgen regulated genes . -
pubmed
Cloning of the complementary DNA encoding the
androgen receptor enabled the characterization of the molecular defects associated with
androgen insensitivity syndromes ,
X - linked disorders resulting from
androgen action defects in target cells . -
pubmed
The cis - effect of a nascent peptide on its translating ribosome : influence of the
cat-86 leader
pentapeptide on translation termination at leader codon 6 . -
pubmed
The inducer
chloramphenicol stalls a ribosome at a specific site in the leader of
cat transcripts ; this destabilizes a downstream stem - loop structure that normally sequesters the ribosome - binding site for the
cat structural gene . -
pubmed
Our evidence indicates that the
cat-86 leader
pentapeptide can alter the function of its translating ribosome . -
pubmed
An absent or negative inotropic response to
isoproterenol is observed in SHR - F and SHR - NF papillary muscles and may result in part from age - related alterations in
beta - adrenergic receptor dynamics and a shift from alpha- to
beta - myosin heavy chain (
MHC ) protein . -
pubmed
The
renin -
angiotensin -
aldosterone system is also implicated in the transition to failure : SHR treated with the
angiotensin converting enzyme inhibitor
captopril starting at 12 months of age did not develop
heart failure during the 18 - 24 month observation period . -
pubmed
Research opportunities include investigation of apoptosis as a mechanism of cell loss , delineation of the regulatory roles of
TGF - beta 1 and the
renin -
angiotensin -
aldosterone system in matrix accumulation , and studies of
proteinase cascades that regulate matrix remodelling . -
pubmed
Such a distribution seems to be responsible for the transcellular transport of
Pgp substrates , including
cyclosporin A (
CsA ) , from the basal to the apical side . -
pubmed
Nevertheless , the sensitivity of their mitogen - induced proliferation to cytostatics , including
doxorubicin and
CsA , could be increased by the
Pgp blockers . -
pubmed
The
Pgp blockers can restore the
CsA retention in the MDR cells to its level in the Par cells . -
pubmed
For instance , activating mutations of the
Gs protein , leading to constitutive stimulation of the
cAMP pathway , have been found in a subset of GH - secreting
adenomas . -
pubmed
But
GHRH (
growth hormone - releasing hormone ) tumoral hypersecretion also stimulates the
cAMP pathway and causes
acromegaly . -
pubmed
On acute stage ,
99mTc -
ECD brain SPECT disclosed diffuse high uptake in the cerebral cortex in spite of normal findings on brain X - ray
CT . -
pubmed
The total cellular
p185 ( HER-2 / neu ) protein (
p185 ) content was measured by ELISA in 346 invasive primary
breast cancers , and the results were compared with those of
estrogen (
ER ) and
progesterone ( PR ) receptors ,
pS2 and
Cathepsin D (
Cat D ) content . -
pubmed
Our results demonstrate for the first time that all
SP - immunoreactive boutons in all cortical layers contain
GABA . -
pubmed
Determination of the amino - terminal
amino acid sequence showed it to be identical to that predicted from the derived sequence for the
argR gene . -
pubmed
Quantitative
S1 nuclease experiments showed that expression of the first gene in this operon ,
aruC , is initiated from an
arginine - inducible promoter . -
pubmed
Our preliminary findings showed that
geiparvarin and some derivatives obtained from its conjugation with
diethylstilboestrol and
meso - hexestrol significantly inhibit
taxol - induced in vitro polymerization of both
tubulin and microtubular protein . -
pubmed
Matrix metalloproteinase-3 (
MP-3 or
stromelysin-1 ) specifically hydrolyzes the
Glu59 -
Asn60 ,
Pro447 - Val448 , and
Pro544 -
Ser545 peptide bonds in
plasminogen , yielding a 55 kDa NH2 - terminal
angiostatin - like domain ( comprising kringles 1 - 4 ) , a 14 kDa domain comprising kringle 5 , and a 30 kDa domain comprising the
serine proteinases domain . -
pubmed
The conversion is completely abolished in the presence of the
MMP inhibitors
EDTA or
1,10 - phenanthroline . -
pubmed
By contrast ,
TNF was a very poor activator of
p42 / 44 MAP kinase relative to the known activator of
p42 / 44 MAP kinase in endothelial cells ,
adenosine triphosphate (
ATP ) . -
pubmed
TNF - stimulated activation of
p38 MAP kinase , and
MAPKAP kinase-2 , a known downstream target of
p38 MAP kinase , was strongly inhibited by pre - incubation with the
p38 MAP kinase inhibitor
SB203580 , whereas the minor activation of
p42 / 44 MAP kinase was abolished by pre - incubation of the cell with the novel
MAP kinase kinase 1 inhibitor
PD098059 . -
pubmed
Pre - incubation with
PD098059 or co - incubation with
ATP failed to modify the inhibitory effect of
TNF upon DNA synthesis . -
pubmed
SB203580 reduced basal DNA synthesis by approximately 50 % ; however , if failed to modify the inhibition mediated by
TNF . -
pubmed
Binding of
CD4 ligands induces
tyrosine phosphorylation of
phosphatidylinositol-3 kinase p110 subunit . -
pubmed
Since these results suggest a relationship between
p56lck and
PI3 - kinase , we investigated whether
PI3 - kinase was
tyrosine phosphorylated after
CD4 binding and whether this phosphorylation was also dependent on the
CD4 -
p56lck association . -
pubmed
We show herein that
CD4 binding increased
tyrosine phosphorylation of the catalytic subunit
p110 of
PI3 - kinase but not of the
p85 subunit . -
pubmed
In contrast ,
p110 tyrosine phosphorylation was inducible , transient and dependent on the
CD4 -
p56lck association . -
pubmed
The role of the
tyrosine phosphorylation of
p110 - PI3 - kinase following ligand binding to
CD4 is unknown . -
pubmed
In muscles with myofiber
atrophy and target fibers , we found an increased expression of the genes encoding for the
ubiquitin -
ATP - dependent proteolytic system . -
pubmed
Comparisons of
nucleotide and deduced
amino acid sequences of
NSP4 genes of virulent and attenuated pairs of group A and C rotaviruses . -
pubmed
It was recently reported that
NSP4 may also function as a viral enterotoxin in the induction of
rotavirus diarrhea by causing
Ca ++ influx in the cytoplasm of the infected cells . -
pubmed
Analysis of the
NSP4 deduced
amino acid sequences showed that only 3 ( Wa ) and 2 ( M and Cowden )
amino acids differed between the virulent and attenuated
strains . -
pubmed
After considering the ratio between the number of T cells expressing Th- (
IFN - gamma)- and Th-2 (
IL-4)-associated
cytokines in each individual , we found that there was a significant imbalance in this ratio , with a predominance of
IFN - gamma - producing T cells over
IL-4 + T lymphocytes during
EtOH intake . -
pubmed
Liver mitochondrial low - Km
aldehyde dehydrogenase (
ALDH2 ,
EC 1.2.1.3 ) , the isoform responsible for the conversion of
acetaldehyde to
acetate , is inhibited by the
sulfoxide bioactivation products of
Et2NC ( O ) SMe ( from the
alcohol aversion drug
disulfiram ) ,
Pr2NC ( O ) SEt ( the herbicide
S - ethyl N , N - dipropylthiocarbamate ) , and
BuNHC ( O ) SMe ( from the fungicide
benomyl ) . -
pubmed
The labeled protein was isolated by isoelectric focusing ( pI 6.1 ) and
SDS - PAGE ( 54 kDa ) and identified as
ALDH2 by sequencing of peptides from a tryptic digest . -
pubmed
These findings are of interest relative to selective inhibitors and carbamoylating agents for
ALDH2 and to
alcohol aversion upon exposure to herbicides and fungicides . -
pubmed
Ly-49G2 blocks activation mediated by
Ly-49D in an immunoreceptor
tyrosine - based inhibitory motif ( ITIM)-dependent manner . -
pubmed
This general
amino acid control response is based on de - repressed translation of
GCN4 mRNA , which is induced by the activation of the
eIF2alpha kinase ,
GCN2 . -
pubmed
Thus , GI domain - mediated association of
GCN2 to
GCN1 is required for general
amino acid control . -
pubmed
Histological sections and scanning
electron microscopy of
GP / Bc eyes during the normal period of eyelid growth and fusion indicate that
gp /
gp mutant fetuses have deficient upper and lower eyelids ; surface periderm cells that appear to have some role in eyelid growth and fusion are present , but lack a normal " streaming pattern toward the fusion zone . -
pubmed
Both the
catechol - O - methyltransferase (
COMT ) inhibitor
entacapone and the
monoamine oxidase B (
MAO - B ) inhibitor
selegiline are
L - dopa extenders . -
pubmed
or both
entacapone and
selegiline with the
L - dopa /
DDC inhibitor medication . -
pubmed
Oral administration of
TN ( 50 - 100 mg / kg ) dose - dependently prevented
ulcer formation by
HCl /
ethanol ( 36 - 75 % ) , absolute
ethanol ( 43 - 75 % ) ,
HCl -
ethanol /
indomethacin ( 58 - 84 % ) , Pylorus ligation ( 31 - 100 % ) , cold restraint stress ( 68 - 100 % ) and
histamine ( 49 - 100 % ) . -
pubmed
Augmented
histamine - induced gastric acid secretion was reduced from 84 mEq / l in the controls to 45 and 21 mEq / l for the two doses of
TN , with total inhibition of
gastric and duodenal ulcers by the 50 mg / kg dose . -
pubmed
Healing rate of chronic
acetic acid - induced
ulcers was 62 and 83 % , respectively , for the dose of 50 and 100 mg / kg of
TN compared with the controls . -
pubmed
[ The experimental study of
Sr -
HAP on reconstructing
mandibular bone defect ] . -
pubmed
RESULTS :
Sr -
HAP had hardly been rejected by hosts , and in early period after operation the new bone in the
Sr -
HAP was found with great quantities as the degradation of
Sr - HAP much more than that in the pure HA , and the difference of bone quantity can be shown in statistics . -
pubmed
Sr -
HAP has a better biocompatibility , biodegradation than pure HA and a excellent osteoinductivity . -
pubmed
The existence of
Sr -
HAP improves the total new bone quantity and the interface of bone , also prolongs the period of new bone increasing . -
pubmed
Sr -
HAP has a good biodegradation and suitable speed of biodegradating , so the reconstruction effect with
Sr -
HAP is fairly satisfactory . -
pubmed
This study was undertaken to determine whether
casein , compared with its constituent
amino acids , given at the onset of a meal , would influence intake due to
cholecystokinin (
CCK ) or opioid activity . -
pubmed
We investigated the hypothesis that inhaled
nitric oxide (
iNO ) and
hyperoxia each decreases lung
vascular endothelial growth factor (
VEGF ) expression but increases
endostatin and that concurrent administration of both gases will show a greater effect . -
pubmed
VEGF mRNA decreased significantly with
O ( 2 ) and
O ( 2 ) +
NO compared with breathing RA ( p & lt ; or = 0.05 ) . -
pubmed
Lavage fluid
VEGF protein and lung capillary leak rose significantly with
O ( 2 ) and
O ( 2 ) +
NO compared with RA , but
endostatin was unchanged . -
pubmed
Hyperoxia and
iNO suppresses lung
endostatin expression , but
iNO unlike
hyperoxia alone does not alter lung
VEGF production . -
pubmed
This latter effect and that on
VEGF mRNA level but not protein is abrogated by recovery in reduced
F ( I ) O ( 2 ) for 72 h. -
pubmed
Dual
ACE and
NEP inhibitors : a review of the pharmacological properties of
MDL 100240 . -
pubmed
MDL 100240 is a pro - drug that , upon conversion to
MDL 100173 , acts as a potent dual inhibitor of
ACE and
NEP with a balanced activity on both enzymes . -
pubmed
Studies in vivo in experimental models of
hypertension and
congestive heart failure confirmed the vasodilatory and natriuretic effects of
MDL , which appear to be independent of the degree of activation of the
renin -
angiotensin -
aldosterone system . -
pubmed
If the promises of this novel therapeutic strategy are fulfilled , clinical trials are expected to demonstrate advantages of
MDL 100240 over pure
ACE inhibitors . -
pubmed
In order to investigate the conditions necessary for
group III mGluR autoreceptor - activation by synaptically released
glutamate , we characterized the effects of selective agonists and antagonists on excitatory postsynaptic currents ( EPSCs ) evoked by several distinct PF stimulation patterns . -
pubmed
Paired -
pulse or four -
pulse PF stimulations did not activate presynaptic
group III mGluRs as revealed by the lack of effect of 1 mM
MSOP on relative test EPSC amplitudes with latencies of 250 - 500 ms . -
pubmed
Because
GHMP kinases , thus far , have only been characterized as small
molecule kinases involved in metabolic pathways , for example ,
amino acid and
cholesterol synthesis ,
XOL-1 is the first member that controls nonmetabolic processes . -
pubmed
Association of cannabimimetic compounds such as
cannabinoids ,
aminoalkylindoles (
AAIs ) , and
arachidonylethanolamide (
anandamide ) with the
brain cannabinoid ( CB ( 1 ) ) receptor activates
G - proteins and relays signals to regulate neuronal functions . -
pubmed
This study aimed to examine if graft preconditioning with
HO-1 could be substituted by a simple treatment with
heme - degrading products such as
bilirubin . -
pubmed
The
HO-1 - preconditioned grafts ex vivo exhibited a marked improvement of bile output and cell
injury that was cancelled by blocking HO with
zinc protoporphyrin - IX . -
pubmed
Furthermore , a short - term rinse treatment with micromolar levels of
bilirubin attenuated
biliary dysfunction and cell
injury of the grafts both ex vivo and in vivo even without
HO-1 preconditioning . -
pubmed
The protective effects of
HO-1 preconditioning or
bilirubin rinse appeared to involve its inhibitory effects on lipid peroxidation in hepatocytes . -
pubmed
In conclusion , these results suggest that
bilirubin rinse serves as a simple strategy to ameliorate hyperacute oxidative stress and
hepatobiliary dysfunction of the transplanted grafts , mimicking effects of
HO-1 - mediated preconditioning . -
pubmed
[ Incorporation of radioactive
amino acids and
amylase synthesis in pancreatic tissue in vitro ] . -
pubmed
The
H2 concentration in the mill
pot , measured by gas chromatography , increased with grinding time up to a maximum concentration of 156 ppm ( 0.35 micromol ) after 600 min . -
pubmed
The
protein has 1309 non - H atoms , seven S atoms , no
disulfide bonds and no heavy - atom prosthetic group . -
pubmed
In each case , the blood
alcohol level (
BAL ) was determined every 30 minutes using about 0.5 ml whole blood obtained by venipuncture . -
pubmed
However , among the male and female volunteers , oral
fructose intake significantly ( p<0.05 ) increased the blood
ethanol clearance rate by 66.7 and 92.3 % , decreased intoxication time by 41.7 and 40 % , reduced peak
BAL by 18.3 and 0 % , but enhanced blood
ethanol oxidation rate by 71.2 and 66.7 % , respectively . -
pubmed
Risk factors for severe
hepatic cystic disease include aging , female sex , pregnancy , use of exogenous female
steroid hormones , degree of
renal cystic disease , or severity of
renal dysfunction ( in patients with mutations in
PKD1 or
PKD2 ) . -
pubmed
In this paper , we propose a theoretical low - resolution 3D model for
pfSHMT in complex with
glycine and
5 - formyl tetrahydrofolate (
5 - FTHF ) based on homology modeling by multiple alignment followed by intensive optimization , validation and dynamics simulations in water . -
pubmed
Serum levels of lipids [
high - density lipoprotein and
low - density lipoprotein cholesterol ,
triglycerides and
lipoprotein ( a ) ] and hemostatic factors (
prothrombin ,
fibrinogen ,
plasminogen activator inhibitor-1 , and
fibrin D - dimer ) were also measured . -
pubmed
Furthermore ,
isoflavone group experienced statistically significant reductions in plasma concentrations of
ICAM-1 ,
VCAM-1 , and
E - selectin . -
pubmed
Experimental
heart failure caused by prolonged infusion of
norepinephrine is associated with diminished
endothelin induced coronary constricting effect of stimulation of
ET ( A ) receptors and inversion of coronary dilating effect of stimulation of these receptors . -
pubmed
At a higher concentration of
p29 , the cells showed similar and accelerated morphological change , but neither externalized
phosphatidylserine nor
caspase-3 activation was observed . -
pubmed
Gelatinases were identified on the basis of molecular weights and inhibition by
EDTA . -
pubmed
The 272 crossbred heifers were randomly allocated within herds to one of the four following groups : 1 ) silastic
coils , 10 days treatment ( n = 65 ) ; 2 )
Norgestomet implants , 10 days treatment ( n = 71 ) ; 3 )
prostaglandin F(
2alpha ) two injections 11 days apart ( n = 70 ) ; and 4 ) control ( n = 66 ) . -
pubmed
Time - lapse
STM studies of diastereomeric
cinchona alkaloids on
platinum metals . -
pubmed
The adsorption of
cinchonidine (
CD ) and
cinchonine (
CN ) on
Pt ( 111 ) and
Pd ( 111 ) single crystals has been investigated by means of scanning tunneling microscopy (
STM ) in an ultrahigh vacuum system . -
pubmed
The diastereomeric
cinchona alkaloids ,
CD and
CN , which are widely used as chiral modifiers of
platinum group metals in catalytic enantioselective hydrogenation , showed similar adsorption modes and diffusion behavior on
Pt ( 111 ) , except that the flatly adsorbed
CN molecules which were free ( not in a dimer / cluster ) were significantly more mobile than their
CD analogues . -
pubmed
Special emphasis is put on the nonlinear effect observed in hydrogenation reactions with
CD /
CN mixtures . -
pubmed
Our observations corroborate that this effect is mainly a consequence of the different adsorption strengths of
CD and
CN on
Pt . -
pubmed
Design of a functional membrane protein by engineering a
heme - binding site in
glycophorin A. -
pubmed
We have designed a functional model membrane protein by engineering a
bis - Histidine heme - binding site into a natural membrane protein ,
glycophorin A (
GpA ) , structurally characterized by the dimerization of a single transmembrane helix . -
pubmed
Out of the 32 residues comprising the transmembrane helix of
GpA , five
amino acids were mutated ; the resulting protein ,
ME1 , has been characterized in
dodecyl phosphocholin (
DPC ) micelles by UV - vis , CD spectroscopy , gel electrophoresis , and analytical ultracentrifugation . -
pubmed
ME1 binds
heme with sub - micromolar affinity and maintains the highly helical secondary structure and dimeric oligomerization state of
GpA. -
pubmed
The
ME1 -
Heme complex exhibits a redox potential of -128 + /- 2 mV vs
SHE , indicating that the
heme resides in a hydrophobic environment and is well shielded from the aqueous phase . -
pubmed
Moreover ,
ME1 catalyzes the
hydrogen peroxide dependent oxidation of organic substrates such as
TMB (
2,2 ' , 5,5 '- tetramethyl - benzidine ) . -
pubmed
Ketamine in
post - tonsillectomy
pain . -
pubmed
Isoprenoids are synthesized through the condensation of five -
carbon intermediates ,
isopentenyl diphosphate (
IPP ) and
dimethylallyl diphosphate (
DMAPP ) , derived from two distinct biosynthetic routes : cytosolic
mevalonate (
MVA ) and
plastidial 2 - C - methyl - D : - erythritol 4 - phosphate (
MEP ) pathways . -
pubmed
biloba embryo , the transcripts of
GbIDS2 predominantly occurred in the embryo roots , where
diterpene ginkgolides are biosynthesized . -
pubmed
Levels of
PtIDS2 transcripts in the
diterpenoid resin - producing wood were 4 - 5 times higher than those in other tissues . -
pubmed
Higher levels of
GbIDS1 transcripts were induced by light , whereas those of
GbIDS2 were increased by
methyl jasmonate treatment . -
pubmed
Unphosphorylated
RIIalpha ( 90 - 400 ) , a deletion mutant , undergoes major conformational changes as both of the
cAMP - binding domains wrap around the C subunit 's large lobe . -
pubmed
This structure demonstrates the conserved and isoform - specific features of
RI and
RII and the importance of
ATP , and also provides a new paradigm for designing isoform - specific activators or antagonists for
PKA . -
pubmed
Remarkably , insecticidal and hemolytic activities were both dependent on a common , well defined cluster of hydrophilic residues on one
face of the
cyclotide . -
pubmed
We investigated whether 4 weeks of near normalisation of blood
glucose (
BG ) improves the potentiation of
glucose - stimulated
insulin secretion by
GLP-1 . -
pubmed
Beta cell function was investigated before and after
insulin treatment using stepwise
glucose infusions and infusion of saline or
GLP-1 ( 1.0 pmol kg(-1 ) min(-1 ) ) , resulting in supraphysiological total
GLP-1 concentrations of approximately 200 pmol / l. The responsiveness to
glucose or
glucose+
GLP-1 was expressed as the slope of the linear regression line relating
insulin secretion rate ( ISR ) and plasma
glucose concentration ( pmol kg(-1 ) min(-1 ) [ mmol / l](-1 ) ) . -
pubmed
In contrast , near normalisation of blood
glucose improved beta cell sensitivity to
glucose during
glucose+
GLP-1 infusion ( 1.27+/-0.2 before vs 1.73+/-0.31 after ; p<0.01 ) . -
pubmed
Analysis of
RSpo deletion mutants indicates that the
cysteine - rich
furin domains are sufficient and essential for the amplification of Wnt signaling and inhibition of
DKK1 , suggesting that Wnt amplification by
RSpo proteins may be a direct consequence of
DKK1 inhibition . -
pubmed
Modulation of peripheral
serotonin levels by novel
tryptophan hydroxylase inhibitors for the potential treatment of functional
gastrointestinal disorders . -
pubmed
Kinase inhibitor studies with macrophages demonstrated that the
Src family PTKs were required for this
tyrosine phosphorylation activity . -
pubmed
In silico methods and site - directed mutagenesis were used to identify EPIYG and EPVYA motifs in
LspA1 that contained
tyrosines that were targets for phosphorylation . -
pubmed
A total of four
tyrosines could be phosphorylated in
LspA1 , with
LspA2 containing eight predicted
tyrosine phosphorylation motifs . -
pubmed
Macrophage lysates could also
tyrosine phosphorylate the
LspA proteins and an
LspA1 fusion protein via a mechanism that was dependent on the presence of both divalent cations and
ATP . -
pubmed
Nicotine exposure also resulted in significantly reduced pancreatic respiratory chain enzyme activity , degranulation of beta cells , elevated islet oxidative stress and impaired
glucose - stimulated
insulin secretion compared to saline controls at 26 weeks of age . -
pubmed
This paper demonstrated the selective determination of
folic acid (
FA ) in the presence of important physiological interferents ,
ascorbic acid ( AA ) and
uric acid ( UA ) at physiological pH using electropolymerized film of
5 - amino-2 - mercapto-1,3,4 - thiadiazole (
p - AMT ) modified
glassy carbon (
GC ) electrode . -
pubmed
However , the
p - AMT film modified electrode not only separates the voltammetric signals of AA , UA and
FA with potential differences of 170 and 410 mV between
AA - UA and
UA - FA , respectively but also shows higher oxidation current for these analytes . -
pubmed
Magnetoreception through
cryptochrome may involve
superoxide . -
pubmed
Accordingly , we investigate here a possible radical pair - based reaction in the photoreceptor
cryptochrome that reduces the protein 's
flavin group from its signaling state
FADH * to the inactive state
FADH- ( which reacts to the likewise inactive
FAD ) by means of the
superoxide radical ,
O2 * - . -
pubmed
To define group determinants of recognition , we used a model composed of a peptide analogue of the
alpha4 helix , oligonucleotides mimicking processed and unprocessed U5 LTR end and 5 mM
Mg ( 2 + ) . -
pubmed
Proton chemical shift deviations showed that specific binding need conserved
amino acids in the
alpha4 helix and conserved
nucleotide bases and backbone groups at LTR ends . -
pubmed
Impaired S - phase arrest in
acute myeloid leukemia cells with a
FLT3 internal tandem duplication treated with
clofarabine . -
pubmed
We show defects in the response of mutant
FLT3 AML cells to the S - phase drug
clofarabine that could account for the apparent contradiction . -
pubmed
Furthermore ,
cdc25A message levels are maintained by the
FLT3 - ITD , such that
message is reduced by 87.5 % on exposure to
FLT3 small interfering RNA . -
pubmed
Primary
FLT3 - ITD samples from untreated patients also display impaired cell cycle arrest and show enhanced sensitivity on prolonged treatment with
clofarabine compared with wild - type samples . -
pubmed
CONCLUSION : There is a reversal of phenotype in mutant
FLT3 cells dependent on the length of exposure to
clofarabine . -
pubmed
The neurodegenerative changes progressed with age and were accompanied by up - regulation of the
neuronal tissue - type plasminogen activator , a
serine protease known to mediate microglial activation . -
pubmed
In addition , a very active esterifying system , capable of utilizing either
( 3 ) H - retinol or
( 3 ) H - retinol bound to cellular
retinol binding protein as substrates , has been demonstrated in the microsomal fraction of the cell . -
pubmed
Furthermore ,
spinasterol suppressed the lipopolysaccharide ( LPS)-induced expression of pro - inflammatory enzymes and
inflammatory mediators in BV2 microglia . -
pubmed
Spinasterol also suppressed the production of
nitric oxide (
NO ) ,
prostaglandin E2 (
PGE2 ) ,
tumor necrosis factor - alpha (
TNF - alpha ) , and
interleukin-1beta (
IL-1beta ) through
extracellular signal - regulated kinase (
ERK ) pathway - dependent expression of
HO-1 . -
pubmed
Tryptophan fluorescence of the
MaSp - NTDs reveals a change in conformation when pH is decreased , and the pH at which the transition occurs is determined by the amount and type of salt present . -
pubmed
In the current report , we investigated the potency of
DBM ,
DBP , and
DBA in relation to
curcumin for their ability to suppress
TNF - induced
NF - kappaB activation ,
NF - kappaB - regulated gene products , and cell proliferation . -
pubmed
We found that all four agents were active in suppressing
NF - kappaB activation ;
curcumin was most active and
DBM was least active . -
pubmed
When examined for its ability to inhibit the direct DNA binding activity of
p65 , a subunit of
NF - kappaB , only
DBP inhibited the binding . -
pubmed
For inhibition of
TNF - induced
IKK activation ,
DBA was most active . -
pubmed
For suppression of
TNF - induced expression of
NF - kappaB - regulated gene products such as
COX-2 (
inflammation marker ) ,
cyclin D1 (
proliferation marker ) , and
VEGF ( angiogenesis marker ) ,
DBA and
curcumin were more active than
DBM . -
pubmed
Overall , our results indicate that although
curcumin and its analogues exhibit activities to suppress inflammatory pathways and cellular proliferation , a lack of Michael acceptor units in
DBM and
DBP can reduce their activities . -
pubmed
Connexin-43 hemichannels mediate
cyclic ADP -
ribose generation and its
Ca2 + -mobilizing activity by
NAD+/cyclic
ADP -
ribose transport . -
pubmed
The
ADP -
ribosyl cyclase
CD38 whose catalytic domain resides in outside of the cell surface produces the second messenger
cyclic ADP - ribose (
cADPR ) from
NAD (+ ) . -
pubmed
cADPR increases intracellular
Ca ( 2 + ) through the intracellular
ryanodine receptor / Ca ( 2 + ) release channel (
RyR ) . -
pubmed
It has been known that intracellular
NAD (+ ) approaches ecto -
CD38 via its export by connexin (
Cx43 ) hemichannels , a component of gap junctions . -
pubmed
However , it is unclear how
cADPR extracellularly generated by ecto -
CD38 approaches intracellular
RyR although
CD38 itself or
nucleoside transporter has been proposed to import
cADPR . -
pubmed
Moreover , it has been unknown what physiological stimulation can trigger
Cx43 - mediated export of
NAD (+ ) . -
pubmed
Here we demonstrate that
Cx43 hemichannels , but not
CD38 , import
cADPR to increase intracellular
calcium through
RyR. -
pubmed
Protein kinase A (
PKA ) activation also induced
calcium mobilization in the same way as
FcgammaR stimulation . -
pubmed
FcgammaR stimulation - induced
calcium mobilization was blocked by
PKA inhibition , indicating that
PKA is a linker between
FcgammaR stimulation and
NAD(+)/
cADPR transport . -
pubmed
Cx43 overexpression in
Cx43 - negative cells conferred extracellular
cADPR - induced
calcium mobilization by the mediation of
cADPR import . -
pubmed
Our data suggest that
Cx43 has a dual function exporting
NAD (+ ) and importing
cADPR into the cell to activate intracellular
calcium mobilization . -
pubmed
In this study , the
amino acid sequences of
kappa - conotoxin RIIIJ and its four mutageneses were converted into ten
HP sequences according to the normalized hydrophobicity index . -
pubmed
The contribution of
endothelium - derived hyperpolarizing factor (
EDHF ) to endothelium - dependent relaxation was impaired in
uremic arteries after stimulation with
bradykinin , but not
acetylcholine , reflecting the agonist - specific differences . -
pubmed
TP was administered as a continuous intravenous dose of 1.3 mug / kg / h and
dopamine was administered in doses up to 20 mug / kg / min to maintain a mean arterial pressure of 70 + - 5 mm Hg for 48 h. Hemodynamic changes , ratio of partial pressure of arterial
oxygen to fraction of inspired
oxygen (
PaO ( 2 )/ FiO ( 2 ) ) , and
VEGF were recorded prospectively . -
pubmed
VEGF concentrations significantly decreased from baseline levels in the
TP group ( p & lt ; 0.05 ) at 48 h ; there was no difference in the
dopamine group ( p & gt ; 0.05 ) at 48 h vs. baseline . -
pubmed
Our aim was to compare the effects of oral
mycophenolate mofetil (
MMF ) with low - dose
prednisone and the full - dose
corticosteroids (
CS ;
prednisone ) for the induction therapy of
HSPN with large
proteinuria . -
pubmed
RESULTS : At 6 months , the estimated
glomerular filtration rate level remained stable , while the urine protein decreased significantly in both groups , and the remission rate was 76.9 % in the
CS group and 55.5 % in the
MMF group ( p = 0.101 ) . -
pubmed
With a median follow - up of 28.8 months in the
CS group and 28.2 months in the
MMF group , the overall remission rate was 80.8 % in the
CS group and 77.8 % in the
MMF group ( p = 0.788 ) . -
pubmed
The
MMF group had less side effects than the
CS group ( 48.1 vs. 76.9 % , p = 0.031 ) . -
pubmed
The
relapse was 4/21 ( 19.0 % ) in the
CS group and 0/21 in the
MMF group ( p = 0.115 ) . -
pubmed
rhizogenes strain A13 , when cultured in optimized half - strength
MS medium containing 1.5 times the standard amount of
ammonium nitrate and
potassium nitrate and 5 % ( w / v )
sucrose , had exhibited improvement in growth index , that is , the fresh biomass was almost double as compared to its initial growth in unmodified half - strength
MS medium . -
pubmed
A predominance of alpha - helix structures was detected by circular dichroism with thermal stability up to 45 C. The oligomeric assemblage of
SELA was investigated by
glutaraldehyde crosslinking experiments indicate that
SELA homodecameric structure is the result of a stepwise addition of intermediate oligomeric states and not a direct monomer to homodecamer transition . -
pubmed
HER2 Status by Standardized Immunohistochemistry and
Silver - Enhanced In Situ Hybridization in Korean
Breast Cancer . -
pubmed
We investigated the
HER2 status in Korean
breast cancer by immunohistochemistry ( IHC ) and
silver - enhanced in situ hybridization ( SISH ) , as the first step toward building a nationwide quality assurance program for
HER2 testing . -
pubmed
Pectinase was entrapped in
polyvinyl alcohol (
PVA ) sponge and the optimum pH and temperature of the free and immobilized
enzymes were shifted from 4 , 40 C to 6 , 50 C respectively . -
pubmed
Vesicular nucleotide transporter regulates the
nucleotide content in airway epithelial mucin granules . -
pubmed
Nucleotides within the airway surface liquid promote fluid secretion via activation of airway epithelial
purinergic receptors . -
pubmed
We assessed the contribution of the recently identified
SLC17A9 vesicle
nucleotide transporter (
VNUT ) to the
nucleotide availability within isolated mucin granules and further examined the involvement of
VNUT in mucin granule secretion - associated
nucleotide release . -
pubmed
Mucin granules isolated from
VNUT short hairpin RNA - expressing cells exhibited a marked reduction of
ATP ,
ADP ,
AMP , and
UTP levels within granules . -
pubmed
These results suggest that
VNUT is the relevant
nucleotide transporter responsible for the uptake of cytosolic
nucleotides into mucin granules . -
pubmed
By controlling the entry of
nucleotides into mucin granules ,
VNUT contributes to the release of purinergic signaling molecules necessary for the proper hydration of co - released mucins . -
pubmed
RESULTS :
Nicotine abstinence induced a
decrease in heart rate and systolic blood pressure ( BP ) before the
BH procedure ( heart rate : 78.80 + - 11.43 under
nicotine , 70.88 + - 10.83 under placebo ;
systolic BP : 124.90 + - 11.34 under
nicotine , 121.18 + - 13.44 under placebo ) and shorter
BH duration relative to the
nicotine patch condition . -
pubmed
Silibinin meglumine , a water - soluble form of milk thistle silymarin , is an orally active anti -
cancer agent that impedes the epithelial - to - mesenchymal transition (
EMT ) in
EGFR - mutant
non - small - cell lung carcinoma cells . -
pubmed
As a single agent , oral
silibinin meglumine notably decreased the overall volumes of
NSCLC tumors as efficiently as did the
EGFR tyrosine kinase inhibitor ( TKI )
gefitinib . -
pubmed
Because the epithelial - to - mesenchymal transition (
EMT ) is required by a multiplicity of mechanisms of resistance to
EGFR TKIs , we evaluated the ability of
silibinin meglumine to impede the
EMT in vitro and in vivo . -
pubmed
Lincomycin biosynthesis involves a
tyrosine hydroxylating heme protein of an unusual enzyme family . -
pubmed
Mass spectrometry and Soret band - excited Raman spectroscopy of
LmbB2 showed that
LmbB2 contains
heme b as prosthetic group . -
pubmed
The
CO - reduced differential absorption spectra of
LmbB2 showed that the coordination of
Fe was different from that of
cytochrome P450 enzymes . -
pubmed
LmbB2 exhibits sequence similarity to
Orf13 of the
anthramycin biosynthetic gene cluster , which has recently been classified as a
heme peroxidase . -
pubmed
Tyrosine hydroxylating activity of
LmbB2 yielding
DOPA in the presence of
( 6R )- 5,6,7,8 - tetrahydro - L - biopterin (
BH4 ) was also observed . -
pubmed
Synthesis and biological evaluation of novel
tetrahydro - beta - carboline derivatives as antitumor growth and
metastasis agents through inhibiting the
transforming growth factor - beta signaling pathway . -
pubmed
Here we demonstrated the identification of a series of
tetrahydro - beta - carboline derivatives from virtual screening which potentially inhibit the
TGFbeta signaling pathway . -
pubmed
Optimization of the initial hit compound
2 - benzoyl-1,3,4,9 - tetrahydro - beta - carboline ( 8a ) through substitution at different positions to define the structure - activity relationship resulted in the discovery of potent inhibitors of the
TGFbeta signaling pathway . -
pubmed
Increased
CEH expression in the liver significantly increased the flux of HDL - CE to
bile acids . -
pubmed
In the
LDLR(-/- ) background , this enhanced elimination of
cholesterol led to attenuation of diet - induced
atherosclerosis with a consistent increase in fecal
sterol secretion primarily as
bile acids . -
pubmed
Taken together with the observed reduction in
atherosclerosis by increasing macrophage
CEH - mediated
cholesterol efflux , these studies establish
CEH as an important regulator in enhancing
cholesterol elimination and also as an anti - atherogenic target . -
pubmed
As a result of their sponge - like morphology ,
aluminum phosphonates adsorb conductive small molecules such as
phosphonic acids , which results in a very high proton conductivity of approximately 5 x 10(-2 ) S cm(-1 ) at 120 C and 50 % relative humidity (
RH ) . -
pubmed
The results showed an opposite behaviour among
ozone and
NO and
NO2 concentrations in urban and suburban zones , marked by maximums of
ozone ( minimums
NO ( x ) ) in
spring and summer and minimums ( maximums ) in autumn and winter . -
pubmed
During the observation period , the proportion of
pain - free attacks at 2 h did not significantly differ between
frovatriptan and the comparators in either men ( 32 vs. 38 % , p =
NS ) or women ( 30 vs. 33 % , p =
NS ) . -
pubmed
coli on several
carbon sources is dependent on the catabolite repressor / activator (
Cra ) protein although a
Cra consensus DNA - binding site is not present in the control regions of the relevant catabolic operons . -
pubmed
Apocynin is a selective inhibitor of the phagocyte
NADPH oxidase Nox2 that can be applied orally and is remarkably effective at low dose . -
pubmed
The structural optimization and normal mode analysis were performed for model compound C , consisting of
thiophosphoryl core , one branch with three repeated units , and four
4 - oxybenzaldehyde terminal groups on the basis of the density functional theory ( DFT ) at the
PBE / TZ2P level . -
pubmed
The first one -
pot synthesis of metal - organic frameworks functionalised with two
transition - metal complexes . -
pubmed
The synthesis of a metal - organic framework (
UiO-67 ) functionalised simultaneously with two different
transition metal complexes (
Ir and
Pd or
Rh ) through a one -
pot procedure is reported for the first time . -
pubmed
BACKGROUND : Safety and efficacy of
tenofovir disoproxil fumarate (
TDF ) as a component of antiretroviral therapy (
ART ) have been demonstrated in clinical trials . -
pubmed
This meta - analysis explored the frequency of discontinuation attributed to renal adverse events ( AEs ) in randomized , controlled clinical studies that used
TDF - containing regimens for
ART - naive ,
HIV - infected patients . -
pubmed
Phylogenetic trees based on the
amino acid and
nucleotide sequences of
eIF-4A demonstrated a similar topology with the classical systematics , suggesting that it has the potential value in phylogenetic inference of eukaryotes . -
pubmed
T - cell
cytokine gene polymorphisms and
vitamin D pathway gene polymorphisms in
end - stage renal disease due to
type 2 diabetes mellitus nephropathy : comparisons with health status and other main causes of
end - stage renal disease . -
pubmed
BACKGROUND : T - cell
cytokine gene polymorphisms and
vitamin D pathway gene polymorphisms were evaluated as possibly associated with
end - stage renal disease (
ESRD ) resulting from
type 2 diabetes mellitus (
DM )
nephropathy . -
pubmed
A frequency distribution of T - cell - related interleukin (
IL ) genes (
IL18 rs360719 ,
IL12A rs568408 ,
IL12B rs3212227 ,
IL4R rs1805015 ,
IL13 rs20541 ,
IL28B rs8099917 ,
IL28B , and rs12979860 ) and
vitamin D pathway genes (
GC genes : rs2298849 , rs7041 , and rs1155563 ;
VDR genes : rs2228570 , rs1544410 ; and
RXRA genes : rs10776909 , rs10881578 , and rs749759 ) was compared between groups . -
pubmed
There was an overexpression of inflammatory
cytokines in the mother , such as
IL-6 and
TNF - alpha , and , in addition ,
PGE2 was also increased . -
pubmed
Neonates showed lower levels of
IL-6 and
TNF - alpha and higher values of
sTNF - RII and
PGE2 in comparison with their mothers . -
pubmed
On the basis of quantum chemical calculations , these bands are assigned to a
C2 isomer of
( OCS ) 2 ( + ) , in which an intermolecular semi - covalent bond is formed between the
sulfur ends of the two OCS components by the charge resonance interaction , and the positive charge is delocalized over the dimer . -
pubmed
In addition to the bands of the
OCS (- ) core isomer , we found another band at ~1740 cm(-1 ) , which can be assigned to isomers having an
( OCS ) 2 (- ) ion core ; this dimer core has
C2 symmetry and ( 2)A electronic state . -
pubmed
TTF was significantly longer in
GXR versus placebo ( p = 0.003 ) . -
pubmed
Notably , we did not detect the assumed
GSP - mechanisms driving these changes : despite an increased metabolic rate there was neither an increase of C - rich biomolecules ( instead fat and
sugar contents decreased under predation risk ) , nor a decrease of N - rich proteins . -
pubmed
The concentration of
17beta - estradiol (
E2 ) was measured through stages of wastewater treatment at a central Illinois wastewater treatment plant ( WWTP ) . -
pubmed
The concentration of
E2 was compared with demographic effects of a university ; physical parameters of the wastewater ( dissolved
oxygen , pH , and temperature ) ; and daily influent and effluent flow rates . -
pubmed
The molecular modeling of
PHY US42 indicated that this
phytase belongs to the group of
beta - propeller phytases that are usually
calcium - dependent . -
pubmed
Its activity was
calcium - dependent and maximal at pH 7 and 65 C. The
enzyme was perfectly stable at pH ranging from 5 to 10 and its thermostability was greatly enhanced in the presence of
calcium . -
pubmed
Indeed ,
PHY US42 maintained 80 % of activity after 10 min of incubation at 75 C in the presence of 5 mM
CaCl2 . -
pubmed
Compared with never users , the pooled RRs of PLC were 0.60 ( 95 % CI 0.37 , 0.96 ) for ever users of
MHT , 0.73 ( 95 % CI 0.46 , 1.17 ) for ever users of
estrogen - only therapy (
ET ) and 0.67 ( 95 % CI 0.45 , 1.02 ) for ever users of
estrogen -
progestin therapy ( EPT ) . -
pubmed
Also
25 hydroxyvitaminD (
25 - OH - VitD ) ,
parathyroid hormone (
iPTH ) , posphorus , serum
uric acid , renal resistive index ( RRI ) and
C - reactive protein (
CRP ) showed a significant difference between the two groups ( p=0.002 , p<0.001 , p<0.001 , p<0.001 , p<0.001 , p<0.001 ) . -
pubmed
Paracetamol was the most common drug used for self - medication 31 % , followed by
Azithromycin 17.6 % and combination of
Paracetamol and
Ibuprofen 15.6 % ,
Cetirizine 8.6 % ,
Amoxicillin 6.5 % ,
Omeprazole 6.3 % ,
Albendazole 3.3 % ,
Mefenemic acid 2.8 % ,
Cefpodoxime2 % respectively . -
pubmed
Here , a focused metabolomics approach was used to identify changes in
folate catabolism as well as the
S - adenosylmethionine (
SAM ) cycle following
NAT1 knockdown with shRNA . -
pubmed
By contrast , the flux of
homocysteine in the medium was different following
NAT1 knockdown after the
methionine content was exhausted suggesting a need for this metabolite in
methionine synthesis . -
pubmed
RESULTS : The inflection point of serum
25 - OHD level for maximal suppression of
PTH was at 18.0 ng / mL ( 95 % confidence interval , 14.3 - 21.7 ng / mL ) . -
pubmed
Gamma - hydroxybutyrate (
GHB ) is a
GHB -/ GABA - B receptor agonist inducing a broad spectrum of subjective effects including
euphoria , disinhibition , and enhanced vitality . -
pubmed
A whole - brain analysis showed increased rCBF in the bilateral anterior cingulate cortex (
ACC ) and the right anterior insula under
GHB ( p<0.05 , cluster - corrected ) . -
pubmed
Interaction analyses revealed that
GHB - induced
increase of body awareness was accompanied by increased rCBF in
ACC , whereas relaxation under
GHB was accompanied by elevated rCBF in right anterior insula ( p<0.05 , uncorrected ) . -
pubmed
In conclusion , enhancement of emotion and body awareness , and increased perfusion of insula and
ACC bears implications both for the properties of
GHB as a drug of abuse as well as for its putative personalized potential for specific therapeutic indications in
affective disorders . -
pubmed
Inhibition of cell proliferation through an
ATP - responsive co - delivery system of
doxorubicin and
Bcl-2 siRNA . -
pubmed
Herein , DNA duplex was constructed through the hybridization of
adenosine triphosphate (
ATP)-responsive aptamer and its cDNA in which
GC - rich motif could be used to load
doxorubicin (
DOX ) , and then , cationic polymer
PEI25 K was used as a carrier to simultaneously condense
DOX - Duplex and
Bcl-2 siRNA to prepare the ternary nanocomplex
polyethylenimine (
PEI)/
DOX - Duplex / siRNA . -
pubmed
Overall , the
ATP - responsive nanocarrier for co - delivering
DOX and
Bcl-2 siRNA has been demonstrated to be a smart delivery system with favorable anti - proliferative effect , especially for solving the multidrug resistance of
tumors . -
pubmed
The bulk filtered
phosphate is reabsorbed in the renal proximal tubule by two specialized
phosphate transporters ,
NaPi - IIa and
NaPi - IIc . -
pubmed
Phosphate balance is regulated by dietary
phosphate intake , and by the action of the
parathyroid hormone ,
vitamin D3 and
fibroblast growth factor-23 (
FGF-23 ) . -
pubmed
All these regulators exert their effect by modulating the activity of the proximal - tubular
phosphate transporters ,
NaPi - IIa and
NaPi - IIc . -
pubmed
Arbutin (
p - hydroxyphenyl - beta - D - glucopyranoside ) , a well - known
tyrosinase inhibitor , has been widely used as a cosmetic whitening agent . -
pubmed
The underlying molecular mechanism for these effects involves decreased levels of reactive
oxygen species ( ROS ) , improvement of
daf-16 nuclear localization , and up - regulated expression of
daf-16 and its downstream targets , including
sod-3 and
hsp16.2 . -
pubmed
Though not natural minerals themselves , both compounds adopt the
pavonite structure , which crystallizes in the
C2 / m space group and consists of two connected , alternating defect rock - salt slabs of varying thicknesses to create a three - dimensional lattice . -
pubmed
15 - hydroxyprostaglandin dehydrogenase (
15 - PGDH ) is a
prostaglandin metabolizing enzyme that oxidizes the
hydroxyl group at
carbon 15 (
C15 ) . -
pubmed
The aim of the present work is to propose the main
amino acids that catalyze the reaction through studying the intermolecular interaction between the ligand and the
enzyme inside the active site using molecular dynamics simulation ( MD ) . -
pubmed
Therefore , MD simulations for two
15 - PGDH systems bound with a substrate (
PGE2 ) or an inhibitor ( compound 4 ) were performed to investigate the importance of ligand interaction on the behavior of
amino acids in the active site . -
pubmed
Among the emerging new psychoactive substances (
NPS ) , compounds carrying an
N - ortho - methoxybenzyl substituent , the so - called
NBOMes , represented a highly potent group of new hallucinogens . -
pubmed
Bioanalytical method validation as per
EMA guidelines was carried out where the proposed method revealed linearity over the concentration range of 5 - 5000 and 10 - 1500 ng mL-1 for
sofosbuvir and
velpatasvir , respectively . -
pubmed
The ratio of 40K/232Th and
40 K /238U were calculated , which indicates the presence of 5.79 and 2.69 times more K - bearing minerals as compared with
Th and U - bearing minerals in the soil samples , respectively . -
pubmed
For that , primary cortical cultures exposed to
oxygen and
glucose deprivation (
OGD ) were used as a model . -
pubmed
In this study , we also demonstrate that selective activation of
GPER induced astrocyte apoptosis via the
phospholipase C pathway and subsequent intracellular
calcium rise , whereas in neurons , this effect was not observed . -
pubmed
The enzymatic determination of
glucose in soft drinks , based on the
glucose concentration measurement by means of the coupled reactions of
glucose oxidase and
peroxidase , has been implemented at the University of Malaga and optimized according to the Biochemistry and Chemistry undergraduate students ' results and feedback throughout the last few years . -
pubmed
Two identical
CAD / CAM
titanium abutments only differing in color ( gray and pink ) were fabricated along with an all - ceramic
zirconia crown . -
pubmed
Under
AM 1.5 illumination ( 100 mW / cm2 ) conditions , the optimized
PCBM :
P3HT solar cells with a chemical bath deposited a
CdS layer of thickness 70 nm and showed 50 % power conversion efficiency enhancement , in comparison with similar solar cells with optimized dense
TiO2 of 50 nm thickness prepared by spray pyrolysis . -
pubmed
Conclusion : These data suggested that female sexual
steroids have a therapeutic effect on gastrointestinal
ischemic disorders by reduction of
MPO and gastric acid , and increasing gastric
GSH & amp ;
SOD levels following gastric IR . -
pubmed
Triiodothyronine ( T3 ) and
estrogen (
E2 ) play important roles in the bone remodeling process and signaling of
receptor activator of the nuclear factor - kappa beta (
RANKL ) and
osteoprotegerin (
OPG ) expressed by osteoblasts . -
pubmed
Glutathione S - transferase mu (
GSTM ) belongs to a family of phase
II detoxification enzymes that catalyze the conjugation of
reduced glutathione (
GSH ) to a wide range of endogenous and exogenous electrophilic compounds . -
pubmed
MTS assays , Transwell assays , and flow cytometry were used to explore the function of
GSTM1 in vitro . -
pubmed
In vitro , Transwell and
doxorubicin (
DOX)-induced apoptosis assays revealed that
GSTM1 showed opposite functions in different
HCC cell lines with varied
TP53 genotype statuses . -
pubmed
The overexpression of
GSTM1 in the above cell lines led to a significant decrease in ROS and an increase in
GSH concentration and
TP53 levels , suggesting that the controversial role of
GSTM1 resulted from the
TP53 genotype of
HCC cells . -
pubmed
RecIL-37 treatment increased endurance exercise capacity by 2.4 - fold , which was accompanied by a 2.9 - fold increase in the phosphorylated
AMP - activated kinase (
AMPK ) to
AMPK ratio ( i.e. -
pubmed
RecIL-37 treatment also improved whole - body
insulin sensitivity and
glucose tolerance ( p & lt ; .05 vs. vehicle ) . -
pubmed
Plasma metabolomics analysis revealed that
recIL-37 treatment altered metabolites related to pathways involved in
NO synthesis ( e.g. -
pubmed
Nor1 , the third member of the Nr4a subfamily of nuclear receptor , is garnering increased interest in view of its role in the regulation of
glucose homeostasis . -
pubmed
Mitochondrial localization of
Nor1 reduced
glucose oxidation , lowered
ATP production rates , and inhibited
glucose - stimulated
insulin secretion . -
pubmed
Graphical abstract Schematic representation of fabrication for porous hydrophilic affinity beads ( pGC ) with honeycomb - biomimetic microchannels based on
graphene oxide ( GO ) and chitosan (
CS ) . -
pubmed
Overexpression of
Sox4 promoted cardiomyocyte apoptosis with or without
H2 O2 , whereas knocking down of
Sox4 alleviated
H2 O2 -induced apoptosis in cardiomyocytes . -
pubmed
Mechanistically , there is a potential
Sox4 - binding site in the promoter region of
Bim , and forced expression of
Sox4 significantly promoted
Bim expression in cultured cardiomyocytes with or without
H2 O2 , whereas knocking down of
Sox4 inhibited the expression of
Bim . -
pubmed
Positive allosteric modulators ( PAMs ) of
GABAB receptor represent an interesting alternative to receptor agonists such as
baclofen , as they act on the receptor in a more physiological way and thus are devoid of the side effects typically exerted by the agonists . -
pubmed
Based on our interest in the identification of new
GABAB receptor PAMs , we followed a merging approach to design new chemotypes starting from selected active compounds , such as
GS39783 ,
rac - BHFF , and
BHF177 , and we ended up with the synthesis of four different classes of compounds . -
pubmed
CONCLUSIONS : The
BPA introduced in our DKA management algorithm successfully reduced total time on
insulin and the incidence of
hypoglycemia . -
pubmed
Relationship between
amyloid and
tau levels and its impact on
tau spreading . -
pubmed
Positive reactions of
3beta - steroid - dehydrogenase and enzymes of the
pentose phosphate cycle indicate
steroid hormone production ; the pathways of the
steroid synthesis , however , are probably different in adult and immature testes . -
pubmed
What is the clinical importance of alterations of the
hemoglobin oxygen affinity in preserved blood -- especially as produced by variations of red cell
2,3 DPG content ? -
pubmed
The antibody against
hepatitis B surface antigen (
anti - HBs ) content of two of the administered batches of
immune human serum globulin was 1:32 and 1:64 . -
pubmed
None of the babies became
hepatitis B surface antigen carriers , and
anti - HBs developed without obvious clinical
hepatitis in one baby . -
pubmed
Conventional
immune human serum globulin in larger doses may be a relatively safe and effective prophylaxis against the development of
hepatitis B surface antigen carrier state even if the
anti - HBs content in the administered dose is relatively small . -
pubmed
The effects of four
calcium channel blockers (
nicardipine ,
nifedipine ,
verapamil , and
diltiazem ) on free radical
injury in cultured endothelial cells were studied and compared with those of
butylated hydroxytoluene . -
pubmed
SP and
capsaicin , but not MC increased the concentration of
FITC - albumin in the nasal fluid . -
pubmed
Scintigraphy with
99mTc ( V )- DMSA demonstrated uptake in medullary
carcinoma of the thyroid gland (
MCT ) . -
pubmed
Iodine-131 metaiodobenzylguanidine (
MIBG ) scintigraphy showed the bilateral
pheochromocytomas but did not demonstrate uptake in the
MCT . -
pubmed
Successful execution of the procedure requires attention to technical details such as the fixation of tissue , use of proteolytic enzymes such as
trypsin for
formalin - fixed tissues , and the choice of the appropriate antibody and controls . -
pubmed
Large
tumours are rich in
necrotic cells , and
cathepsin D and
serine -
proteases are the main hydrolytic enzymes involved in the proteolytic action on melanosomes . -
pubmed
Basic hemodynamic parameters and the state of the
renin -
aldosterone system were determined . -
pubmed
We purified
NF - kappa B over 50,000 - fold and identified two protein species , 42 and 44 kDa , that could be eluted and renatured from a
sodium dodecyl sulfate / polyacrylamide gel to give specific DNA - binding activity . -
pubmed
One of these new derivatives ,
FVIII delta II , in which
amino acids 771(pro)-1666(
asp ) have been deleted , no longer contains the
protease cleavage site at
amino acid position 1648 ( arg )- 1649 ( glu ) known to be involved in the initial step of
FVIII processing . -
pubmed
FVIII delta II has the following properties : ( i ) it exhibits
FVIII procoagulant activity ; ( ii ) it is expressed at 5 - fold higher levels than is the complete molecule in comparable systems ; ( iii ) it migrates for the most part as a single major band on
SDS - PAGE , in contrast to the complete molecule ; ( iv ) it is activated to a greater extent by
thrombin than is either
rFVIII or
pdFVIII ; and ( v ) it retains the ability to bind
von Willebrand factor (
vWf ) . -
pubmed
The time course of vesicle volume is determined by the self - quenching of entrapped
fluorescein sulfonate (
FS ) , the best of a series of dyes screened for self - quenching , brightness , and vesicle loading / trapping . -
pubmed
Urea Ps was 2 x 10(-6 ) cm / s with sigma 0.95 - 1.00 on the basis of the fluorescence time course analysis and the extravesicular [
urea ] required to obtain zero initial volume flow ( null method ) when vesicles were loaded with
sucrose.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
[ Correlation of
prolactin ,
sodium ,
potassium and
calcium in the amniotic fluid , in the mother and in the newborn infant ] . -
pubmed
An analog of
ACTH /
MSH ( 4 - 9 ) ,
ORG-2766 , reduces cerebral uptake of
morphine . -
pubmed
The results explain , in part , how some fragments and analogs of ACTH /
MSH may antagonize behavioral actions of
morphine , even though some of these peptides lack significant
opiate receptor binding properties . -
pubmed
In the fractured
face of dispersions composed of egg yolk
phosphatidylcholine and
glucosyl dipalmitylglycerol , particles having a diameter of 3 - 6 nm , instead of tubular structures , were observed , suggesting that the occurrence of tubular structure may depend on the
fatty acid compositions of the mixtures . -
pubmed
A mixture of
dipalmitoylphosphatidylcholine and
galactosylceramide did not show any distinct tubular or globular structures on the fractured
face . -
pubmed
Leukocyte interferons eluting predominantly in 0.5 M
NaCl buffer , 2 . -
pubmed
Lymphoblastoid interferons eluting mostly in 1 M
NaCl buffer , and 3 . -
pubmed
Cell culture - derived fibroblast and
bladder carcinoma interferons requiring
ethylene glycol in addition to 1 M
NaCl for elution . -
pubmed
Desorption behavior from Blue Sepharose did not necessarily correlate with the presence of specific antigenic markers and no consistent segregation of
Le and
F antigens in individual fractions was observed . -
pubmed
Direct sequencing of the
PLP gene and
PLP mRNAs from the brain of the
PMD patient revealed a G to T transition in exon V of the
PLP gene , which leads to a
glycine to cysteine substitution at residue 220 . -
pubmed
AFP has been shown to bind various ligands in vitro including
fatty acids ,
estrogens ,
thyroid hormones and
retinoic acids . -
pubmed
The presence of nine such hydrophobic repeats in the third domain of the
AFP molecule mimics the heptad dimerization repeats found in the
retinoic acid , thyroid ,
c - erbA and other members of the
nuclear receptor superfamily . -
pubmed
Computer analysis revealed that the most conservative matching occurred between
AFP and the
retinoic acid class of receptors . -
pubmed
Hypocalcemia and an elevation in circulating
1,25 ( OH ) 2D3 in ex
ovo embryos were observed by 15 days . -
pubmed
Calcium and
phosphorus concentrations in femora and tibiae in ex
ovo embryos were significantly lower compared to their normal counterparts . -
pubmed
The
CRT group exhibited a " flattened "
hypotensive portion of the carotid
sinus - heart rate baroreflex curve , but this appeared to be due to the increased neck muscle mass of the subjects . -
pubmed
Characteristics of the inhibition of
NADPH oxidase activation in neutrophils by
apocynin , a
methoxy - substituted catechol . -
pubmed
Phagocytes are able to generate reactive
oxygen species by an activatable
NADPH oxidase system . -
pubmed
We investigated the inhibition of
NADPH oxidase activation by a
methoxy - substituted catechol ,
apocynin . -
pubmed
The lagtime of effect of
apocynin in neutrophils relatively deficient of
myeloperoxidase was about 50 % longer when compared with normal cells . -
pubmed
Immunoblots of neutrophil membranes , isolated at 2 and 7 min after
STZ stimulation of neutrophils , demonstrated translocation of the
cytosolic oxidase components
p47 - phox and
p67 - phox to the membrane fraction . -
pubmed
These properties suggest that
apocynin is an intracellular inhibitor of the assembly of
NADPH oxidase in neutrophils and eosinophils and that
apocynin requires conversion by
peroxidases to exert its inhibitory effect . -
pubmed
The potential therapeutic value of
apocynin was demonstrated in vitro by its ability to protect
secretory leukocyte proteinase inhibitor from oxidative inactivation by neutrophils . -
pubmed
The
cell surface antigen has an
amino acid sequence homologous to
Aplysia ADP - ribosyl cyclase that catalyzes the conversion of
NAD to
cyclic ADP - ribose with a
calcium - mobilizing activity . -
pubmed
A putative
hyaluronate ( HA)-binding motif which has recently been identified in
CD44 antigen existed in the extracellular domain and intracellular
amino terminus of
CD38 antigen . -
pubmed
Effects of culture with
unsaturated fatty acids on
lymphocyte protein kinase activities . -
pubmed
Substance P and
vasopressin levels in the perfusate were the same in controls and after sympathectomy . -
pubmed
He remained
insulin - independent , with normal fasting blood
glucose and
hemoglobin A1c levels , for 21/2 years . -
pubmed
Preoperatively , the acute
insulin response and the rate of
glucose disappearance ( Kg ) were 213 microU / mL and 2.14 % ( respectively ) after intravenous administration of 20 g of
glucose . -
pubmed
Five years posttransplantation he remains
insulin - dependent with a fasting
serum C - peptide level of 0.20 ng / mL , which increased to 0.35 ng / mL in response to intravenous
arginine , indicating sustained islet function . -
pubmed
These changes are dependent on
calcium mobilization and
calcineurin activation , since they are also elicited by
ionomycin and are blocked by the immunosuppressive drug
cyclosporin A. -
pubmed
Deactivation of
NFAT1 is facilitated by
phorbol 12 - myristate 13 - acetate and inhibitors of capacitative
calcium entry and most likely reflects the slow return of intracellular free
calcium concentrations towards resting levels . -
pubmed
Our results suggest that
calcineurin - dependent signalling pathways mediate the early activation of
NFAT1 , while
phorbol 12 - myristate 13 - acetate - dependent feedback pathways contribute to the late deactivation . -
pubmed
Pleural macrophages were isolated 72 h after intrapleural
TCN or intrapleural carrageenan (
CAR ) , a substance that recruits pleural macrophages without producing
pleural fibrosis . -
pubmed
Macrophage cultured for 24 h produced a conditioned medium that was added to pleural mesothelial cell culture containing
[ 3H ]- glucosamine and was compared to control cultures treated with RPMI culture media alone or with the addition of
TCN or
CAR . -
pubmed
After 72 h ,
GAGs were isolated by
pronase digestion ,
cetyl pyridinium precipitation , and
MgCl2 and
ethanol extraction . -
pubmed
Isoproterenol regulates
tumour necrosis factor ,
interleukin-10 ,
interleukin-6 and
nitric oxide production and protects against the development of vascular hyporeactivity in
endotoxaemia . -
pubmed
Pro - inflammatory
cytokines , such as
tumour necrosis factor (
TNF ) and free radicals , such as
nitric oxide (
NO ) , are mediators of
endotoxaemia . -
pubmed
Cyclic AMP can modulate endotoxin - induced
cytokine and
NO production . -
pubmed
The hyporeactivity is due to expression of the inducible isoform of
NO synthase (
iNOS ) and was restored by in vitro administration of
NG - methyl - L - arginine (
L - NMA ) , an inhibitor of
NO synthase . -
pubmed
RESULTS : Serum
sodium and
potassium concentrations decreased during the race , as did serum
total protein ,
albumin , and
globulin concentrations and PCV . -
pubmed
Endocytic uptake of
lysophosphatidylcholine mediated by
macrophage scavenger receptor plays a major role in oxidized low density lipoprotein - induced macrophage growth . -
pubmed
The role of the
methionine loading test (
MLT ) is , however , controversial . -
pubmed
Hyperhomocysteinemia was found in 141 ( 33 % ) of the 429 subjects : 15 % were diagnosed by fasting
homocysteine concentration and 18 % by
MLT . -
pubmed
Folic acid was lower in the group with an elevated fasting
homocysteine concentration than in those with only an abnormal
MLT result ( 11 versus 15 nmol / L , p = 0.002 ) . -
pubmed
In order to characterize
adenosine effects on quiescent peripheral blood mononuclear cells ( PBMC ) , we have evaluated the ability of the previously characterized
adenosine receptor agonist
2 - chloro - adenosine (
2CA ; Ceruti , Barbieri et al . -
pubmed
The effect of
2CA was partially prevented by addition of
dipyridamole (
DP ) , a
nucleoside transport inhibitor , hence some of the apoptotic effect of this
nucleoside is , at least in part , due to intracellular action . -
pubmed
Alternatively ,
DP did not affect
2CdA - induced apoptosis , suggesting that
2CdA may enter cells via a DP - insensitive transporter . -
pubmed
Data suggest that , for
2CA , apoptosis is partially sustained by activation of the
A2A receptor subtype , whereas no role is exerted by
P1 receptors in
2CdA - dependent apoptosis . -
pubmed
Moreover , in these cells , apoptosis could also be triggered through intense activation of the
A3 receptor via selective agonists such as
2 - chloro - N6 -( 3 - iodobenzyl ) adenosine-5 '- N - methyluronamide (
Cl - IB - MECA ) , but this mechanism plays no role in either
2CA- or
2CdA - induced apoptosis . -
pubmed
Rab proteins are geranylgeranylated on one or two C - terminal
cysteines by
Rab geranylgeranyl transferase (
RabGGTase ) . -
pubmed
We measured an equilibrium dissociation constant of 0.2 microM for the formation of a 1:1
REP -
Rab complex and showed that this interaction relies mostly on ionic bonds and does not involve the two C - terminal
cysteine residues . -
pubmed
Third , we show that free
REP inhibits the geranylgeranylation reaction , suggesting that the complex is recognized by
RabGGTase primarily via a REP - binding site . -
pubmed
MPP + decreases the nascent heavy strands per mtDNA and increases the transcript of the
ND6 gene , encoded on light strand , per mtDNA . -
pubmed
Four nodules enhanced by
Gd DTPA were seen at
C1 -
C4 . -
pubmed
In these cells the protein was effectively expressed and
ICA69 carrying C - terminal
histidine - hexapeptide could be efficiently purified using immobilized metal chelate affinity chromatography . -
pubmed
beta - catenin is known as one of the adhesive
molecules associated not only with cell adhesion and cell polarity , but also with carcinogenesis . -
pubmed
The Secretor (
Se ) gene and
Lewis (
Le ) gene are involved in
type I Le antigen synthesis . -
pubmed
The present study was performed to investigate the possibility that
Se and
Le gene polymorphisms alter the risk of
H. - pubmed
Two hundred thirty - nine participants were genotyped for
Se and Le and tested for the presence of
anti - H. pylori IgG antibodies . -
pubmed
Using the
normal gastric mucosa from 60
gastric cancer patients , we assessed immunohistochemically whether
type I Le antigen expression depended on the
Se and Le genotypes . -
pubmed
pylori infection rate was positively associated with the number of
Se alleles ( se /
se group , 45.1 % ;
Se /
se group , 64.6 % ; and
Se /
Se group , 73.3 % ) and negatively associated with the number of
Le alleles (
le /
le group , 76.4 % ; Le /
le group , 68.3 % ; and Le / Le group , 55.6 % ) . -
pubmed
Immunohistochemical analysis supported the finding that
Se and
Le genotypes affected the expression of
H. - pubmed
We conclude that
Se and
Le genotypes affect susceptibility to
H. - pubmed
There was up to a 37 - fold increase in expression of
DR5 following treatment with
ADR or
VP-16 only in cells with wt
p53 . -
pubmed
A direct correlation was observed between enhanced
DR5 expression and sensitivity to
ADR and
VP-16 . -
pubmed
There was no induction of
DR5 following treatment with
VCR ,
MTX or
DEX . -
pubmed
There was up to a 51 - fold increase in the median level of expression of
Fas following treatment with
ADR and
VP-16 , and unlike
DR5 this occurred in cells with either wild - type or mutant
p53 . -
pubmed
Conversely , there was only a two - fold increase in expression of
Fas after exposure to
VCR ,
MTX and
DEX . -
pubmed
These findings suggest that
DR5 mediates sensitivity to
ADR and
VP-16 in a
p53 - dependent manner , whereas ,
Fas appears to mediate sensitivity to these two drugs independent of
p53 status . -
pubmed
DR5 and
Fas do not appear to play a major role as determinants of chemosensitivity to
VCR ,
MTX and
DEX . -
pubmed
Alternatively ,
effector pathways emanating from a specific
Rho GTPase may be under- or overactivated . -
pubmed
In this review , we address the role of the
Rho - type GTPases as a subfamily of the
Ras - superfamily of small
GTP - binding proteins in the development of various disease phenotypes . -
pubmed
Circulating activated platelets (
P - selectin /
CD63 - positive platelets ) increased during
HD sessions with cellulose diacetate and
polysulfone . -
pubmed
Aedes salivary homogenate hydrolyses
inosine and
guanosine to
hypoxanthine and
xanthine plus the
ribose moiety , but does not hydrolyse the
pyrimidines uridine and
cytidine , thus characterizing the presence of a
purine nucleosidase activity . -
pubmed
Thus ,
MsGC - II appears to be a neural - specific receptor guanylyl cyclase whose activity may be regulated either directly or indirectly by
calcium . -
pubmed
RESULTS : The size of the variable region of
AMA-1 gene from
FCC1 /
HN was 506 bp and encoded 168
amino acids . -
pubmed
The levels and relative ratios of
PI3 K products ,
phosphatidylinositol ( 3,4 ) bisphosphate (
PI ( 3,4 ) P ( 2 ) ) and
phosphatidylinositol ( 3,4,5 ) trisphosphate (
PIP ( 3 ) ) , are regulated by
inositol phosphatases such as
Pten and
SHIP . -
pubmed
Here we established a new real - time assay to assess an imbalance of DNA
SSB repair by indirectly measuring
PARP-1 activation through the depletion of intracellular
NAD ( P ) H. -
pubmed
Using this assay , we demonstrated that the dose- and time - dependent depletion of
NAD ( P ) H in
XRCC1 - deficient CHO cells exposed to
methyl methanesulfonate . -
pubmed
Furthermore ,
methyl methanesulfonate reduced
NAD ( P ) H in
PARP-1+/+ cells , whereas
PARP-1-/- cells were more resistant to the decrease in
NAD ( P ) H. -
pubmed
These results indicate that the analysis of intracellular
NAD ( P ) H level using water - soluble
tetrazolium salt can assess an imbalance of
SSB repair in living cells in real time . -
pubmed
When the EG -
VEGF gene - overexpressing
colorectal cancer cell line that had been treated with
phosphorothioate antisense EG -
VEGF oligonucleotides was injected s.c . -
pubmed
Phase II trials using
docetaxel monotherapy have demonstrated responses rates concerning
PSA of 45 to 58 % and objective response of 33 % and 40 % . -
pubmed
A randomized phase
II trial revealed the superiority of this combination compared to
mitoxantrone in terms of response rate and clinical benefit . -
pubmed
Cytochrome P450 2E1 (
CYP2E1 ) enzyme catalyzes the metabolism of many procarcinogens , such as
N - nitrosamines and related compounds . -
pubmed
IL-4 and
IL-13 potentiated
CHX - induced apoptosis in basal cells but not in columnar cells , possibly due to low levels of the
IL-4 receptor . -
pubmed
The initial rate of DNA loss was estimated to be 0.05 bp per chromosome per generation for a WT bacterium and approximately 50 - fold higher for a
mutS mutant defective in
methyl - directed DNA mismatch repair . -
pubmed
The testes were collected in November ( n = 15 ; testis
recrudescence ) , February ( n = 15 ; before breeding season ) , March ( n = 14 ; breeding season ) , and May ( n = 11 ; testis involution ) and the following parameters of testicular activity were quantified : testicular mass , number of testicular spermatozoa , percentages of haploid , diploid , and tetraploid ( G2 / M - phase ) cells and content of
testosterone and
EGF . -
pubmed
Testis mass , spermatogenic activity , and the production of both
testosterone and
EGF were maximal in March , but were not significantly different from the levels in February . -
pubmed
Testosterone and
EGF levels were correlated to each other ( r = 0.78 ; P & lt ; 0.001 ) and had significant positive correlations to testis mass , number of sperm and proportion of haploid cells ; and negative correlations to percentages of mitotic cells . -
pubmed
We inferred that intensified seasonal spermatogenesis was stimulated by
testosterone and by autocrine / paracrine effects of
EGF . -
pubmed
RESULTS : In the challenge model ,
Ciloxan ,
Ocuflox , and endotoxin all produced higher median
DLK scores than the BSS control ( P = .02 ) . -
pubmed
Ciloxan produced significant
DLK in more eyes and had higher median scores ( 70 % , 1.0 ) than
Ocuflox ( 40 % , 0.5 ) or endotoxin ( 45 % , 0.5 ) ( P = .05 ) . -
pubmed
In the endotoxin - induced model ,
Ciloxan produced significantly higher
DLK scores than
Ocuflox or
BSS ( P = .05 ) . -
pubmed
Ocuflox was associated with less
DLK than
Ciloxan . -
pubmed
The activity of the cardiac
Na (+)- Ca ( 2 + ) exchanger (
NCX1.1 ) is allosterically regulated by
Ca ( 2 + ) , which binds to two acidic regions in the cytosolically disposed central hydrophilic domain of the
NCX protein . -
pubmed
However , when cytosolic [
Na (+ ) ] was increased to 20 mm or more ( using
ouabain - induced inhibition of the
Na (+) , K (+)- ATPase or the ionophore
gramicidin ) , cells expressing the D447V mutant rapidly accumulated
Ca ( 2 + ) or
Ba ( 2 + ) when the reverse (
Ca ( 2 + ) influx ) mode of
NCX activity was initiated , although initial cytosolic [
Ca ( 2 + ) ] was & lt ; 100 nm . -
pubmed
Importantly , the time course of
Ca ( 2 + ) uptake did not display the lag phase that reflects allosteric
Ca ( 2 + ) activation of
NCX activity in the wild - type
NCX1.1 ; indeed , at elevated [
Na (+ ) ] , the D447V mutant behaved similarly to the constitutively active deletion mutant Delta(241 - 680 ) , which lacks the regulatory
Ca ( 2 + ) binding sites . -
pubmed
In cells expressing wild - type
NCX1.1 , increasing concentrations of cytosolic
Na (+ ) led to a progressive shortening of the lag phase for
Ca ( 2 + ) uptake . -
pubmed
The results showed that
PC was completely separated from
phosphatidylethanolamine (
PE ) by the elution with 95 %
alcohol , and its purity and yield reached more than 90 % and 80 % with a elution volume of 225 mL , and 87.6 % and 87.3 % with a elution volume of 425 mL respectively . -
pubmed
Arbuscular mycorrhiza (
AM ) was greatly reduced by
CdCl ( 2 ) amendment , and fluorescent pseudomonad inoculation significantly increased
AM colonisation in the contaminated soil . -
pubmed
Here we report the crystal structure at 3.2 - A resolution of an
atToc33 mutant ,
atToc33(R130A ) , in which
Arg(130 ) was mutated to
alanine . -
pubmed
Molecular modeling using the structures of psToc34 and
atToc33(R130A ) suggests that , in an architectural dimer of
atToc33 ,
Arg(130 ) from one monomer interacts with the
beta - phosphate of
GDP and several other
amino acids of the other monomer . -
pubmed
These results indicate that
Arg(130 ) is critical for dimer formation , which is itself important for
GTPase activity . -
pubmed
Neither
Na(+ ) nor
Cl (- ) appears to be a transported substrate for
Bor1p . -
pubmed
Uphill efflux of
boron mediated by
Bor1p was demonstrated directly by loading cells with
boron and resuspending in a low -
boron medium . -
pubmed
Cells with intact
BOR1 , but not the deletant
strain , transport
boron outward until the intracellular concentration is sevenfold lower than that in the medium . -
pubmed
Boron efflux through
Bor1p is a saturable function of intracellular
boron ( apparent K(m ) approximately 1 - 2 mM ) . -
pubmed
Addition of 30 mM
HCO ( 3 )(- ) does not affect
boron extrusion by
Bor1p , indicating that
HCO ( 3 )(- ) does not participate in
Bor1p function . -
pubmed
Functional
Bor1p is present in cells grown in medium with no added
boron , and overnight growth in 10 mM
H ( 3 ) BO ( 3 ) causes only a small increase in the levels of functional
Bor1p and in
BOR1 mRNA . -
pubmed
Tight
glucose control by intensive
insulin treatment markedly improves clinical outcome . -
pubmed
OBJECTIVE AND DESIGN : This is a randomized controlled trial comparing blood
glucose control by a laptop - based model predictive control algorithm with a variable sampling rate [ enhanced model predictive control ( eMPC ) ; version 1.04.03 ] against a routine
glucose management protocol (
RMP ) during the peri- and postoperative periods . -
pubmed
RESULTS : Mean blood
glucose was 6.2 + /- 1.1 mmol / liter in the eMPC vs. 7.2 + /- 1.1 mmol / liter in the
RMP group ( P & lt ; 0.05 ) ; percentage of time in the target range was 60.4 + /- 22.8 % for the eMPC vs. 27.5 + /-
16.2 % for the
RMP group ( P & lt ; 0.05 ) . -
pubmed
Mean
insulin infusion rate was 4.7 + /- 3.3 IU / h in the eMPC vs. 2.6 + /- 1.7 IU / h in the
RMP group ( P & lt ; 0.05 ) . -
pubmed
In a survey of 637 children , 603 ( 95 % ) were positive for antibody to
hepatitis surface antigen (
anti - HBs ) without concomintant antibody to
hepatitis B core antigen (
anti - HBc ) or
hepatitis B surface antigen (
HBsAg ) . -
pubmed
The regulatory effect of
substance P on respiration is mediated via
neurokinin ( NK ) receptors . -
pubmed
Calcium - dependent gating of the large - conductance
Ca ( 2 +)-activated
K (+ ) ( BK(
Ca ) ) channel is conferred by the large cytosolic
carboxyl terminus containing two domains of the regulator of
K (+ ) conductance (
RCK ) and the high - affinity
Ca ( 2 +)-binding site ( the
Ca ( 2 +)-bowl ) . -
pubmed
The mood stabilizers
lithium and
valproate selectively activate the promoter
IV of
brain - derived neurotrophic factor in neurons . -
pubmed
Surprisingly ,
lithium- or
VPA - responsive element(s ) in promoter IV resides in a region upstream from the
calcium - responsive elements ( CaREs ) responsible for depolarization - induced
BDNF induction . -
pubmed
Moreover , activation of
BDNF promoter
IV by
lithium or
VPA occurred in cortical neurons depolarized with
KCl , and deletion of these three CaREs did not abolish
lithium- or
VPA - induced activation . -
pubmed
Lithium and
VPA are direct inhibitors of
glycogen synthase kinase-3 (
GSK-3 ) and
histone deacetylase (
HDAC ) , respectively . -
pubmed
We showed that
lithium - induced activation of promoter
IV was mimicked by pharmacological inhibition of
GSK-3 or short interfering RNA ( siRNA)-mediated gene silencing of
GSK-3alpha or
GSK-3beta isoforms . -
pubmed
Furthermore , treatment with other
HDAC inhibitors ,
sodium butyrate and
trichostatin A , or transfection with an
HDAC1 - specific siRNA also activated
BDNF promoter
IV . -
pubmed
Our study demonstrates for the first time that
GSK-3 and
HDAC are respective initial targets for
lithium and
VPA to activate
BDNF promoter
IV , and that this
BDNF induction involves a novel responsive region in promoter
IV of the
BDNF gene . -
pubmed
The 15 kDa avidin monomer was fused to the
carboxyl terminus of the heavy chain of the
HIRMAb . -
pubmed
Concentrations of the
triamine spermidine and the
tetramine spermine , as well as
ornithine decarboxylase and
S - adenosyl - methionine decarboxylase activities , decreased during sclerotia maturation . -
pubmed
With a
digoxigenin - labeled
KS gene fragment used as a probe , a partial
polyketide synthase (
PKS ) gene cluster was isolated and identified by hybridization screening of a marine sediment sample metagenome fosmid library constructed for this study . -
pubmed
As for intermolecular interactions , we find a very significant difference in the evolving solvation pattern of the nucleophilic
oxygen in water and in the
enzyme . -
pubmed
Cyfluthrin (
CAS no . -
pubmed
Mitotic index ( MI ) , proliferation index (
PI ) and nuclear division index ( NDI ) decreased significantly ( P<0.05 ) due to the potential
cytotoxicity of
cyfluthrin , especially after the 48 - h treatment period . -
pubmed
AIM : To evaluate the chemical activity of Portland cement and two other cement types with similar chemical composition to mineral
trioxide aggregate with the aim of developing these cements for further applications in dentistry . -
pubmed
Elevated serum
triglyceride for men and low serum
high density lipoprotein in women were the strongest predictors that effectively indicated the presence of
metabolic syndrome in non - obese individuals . -
pubmed
Release of
nerve growth factor from
HEMA hydrogel - coated substrates and its effect on the differentiation of neural cells . -
pubmed
In this study , three different types of
poly ( 2 - hydroxyethyl methacrylate ) (
pHEMA ) hydrogels were developed and assessed for storage capacity and release rates of the
neurotrophin ,
nerve growth factor (
NGF ) . -
pubmed
When targeted media concentrations were the same , responses to bath - applied
NGF and
NGF released from
pHEMA hydrogels were not significantly different . -
pubmed
When
NGF was released from
lysine - conjugated
pHEMA hydrogels , a significant increase in process growth was observed . -
pubmed
Our studies demonstrate that
pHEMA coatings can be used on neural devices consistent with the needs for local
neurotrophin delivery in the brain . -
pubmed
Inhibition and binding kinetics of the
hepatitis C virus NS3 protease inhibitor
ITMN-191 reveals tight binding and slow dissociative behavior . -
pubmed
Here ,
ITMN-191 is shown to be a potent inhibitor of
NS3 with a two - step binding mechanism . -
pubmed
From progress curve analysis , the rate constant for dissociation of
ITMN-191 from the
EI * complex is 3.8 x 10(-5 ) s(-1 ) with a calculated complex half - life of approximately 5 h and a true biochemical potency ( K(i ) * ) of approximately 62 pM. -
pubmed
Abrogation of the tight binding and slow dissociative properties of
ITMN-191 is observed with
proteases that carry the R155 K or D168A substitution , each of which is likely in drug resistant mutants . -
pubmed
Naltrexone has higher affinity for
mu receptors than other
opioid receptor subtypes . -
pubmed
Thus , the effect of
naltrexone in treating
SIB appears to be due to (
mu - receptor interactions , with the loss of effect at higher doses due to supervening
kappa receptor binding . -
pubmed
Speculatively , one may ask if dosage reduction would convert
naltrexone nonresponders to responders by lowering
kappa receptor - mediated effects . -
pubmed
In this study , in order to measure the noise - enhancing factor ( NEF ) , a nonlinear stochastic model is introduced for the Hodgkin - Huxley (
HH ) neuron system with synaptic noise input stimulation and channel noises in the
sodium and
potassium channels . -
pubmed
Apoptosis was assessed using terminal
digoxigenin - labeled
dUTP nick - end labeling ( TUNEL assay ) and cell proliferation using
MIB-1 monoclonal antibody by the
alkaline phosphatase method . -
pubmed
Our data suggest that
TIMP-3 interacts with heparan
sulfate and
heparan sulfate proteoglycans and to a lesser extent with heparin and
chondroitin sulfate . -
pubmed
Aldose reductase (
AR ) is a multifunctional enzyme that reduces
aldehydes . -
pubmed
Under diabetic conditions
AR converts
glucose into
sorbitol , which is then converted to
fructose . -
pubmed
In contrast , a recent report suggests that genetic ablation of
AR increased
atherosclerosis and increased
hydroxynonenal in arteries . -
pubmed
It was hypothesized that
AR knockout prevented reduction of toxic
aldehydes . -
pubmed
The effect of
ethanol and heat on the functional hydrophobicity of
casein micelles . -
pubmed
The changes to the hydrophobicity of milk
proteins during that process were evaluated by using the fluorescent hydrophobic probe
1 - anilinonaphthalene-8 - sulfonic acid (
ANS ) . -
pubmed
This resulted in a reduction in the functional hydrophobicity of the milk
proteins as
ethanol rendered the hydrophobic sites unavailable for interaction . -
pubmed
The food - associated fungal neurotoxin
ochratoxin A inhibits the absorption of
glutamate by astrocytes through a decrease in cell surface expression of the excitatory
amino - acid transporters
GLAST and
GLT-1 . -
pubmed
Although
OTA inhibits
glutamine synthetase activity , this effect was not involved in
OTA - mediated alteration of
glutamate absorption since decrease in enzyme activity only occurred at high cytotoxic concentrations of toxin ( 100 microM ) . -
pubmed
Similarly , alterations in the expression of the excitatory
amino - acid transporters were not involved since
OTA failed to modify total expression level of
GLAST and
GLT-1 . -
pubmed
We found that inhibition of
glutamate absorption by
OTA was due to a decrease in the expression of
GLAST and
GLT-1 at the cell surface . -
pubmed
Carboxyl ester lipase (
CEL ) is an enzyme that hydrolyzes a wide variety of lipid substrates , including
ceramides , which are known to show inhibitory regulation of pituitary hormone secretion in experimental models . -
pubmed
Considering that
CEL hydrolyzes
ceramides , inactivating their inhibitory function on pituitary hormone secretion , our findings suggest a possible role of
CEL in the regulation of hormone secretion in both normal and adenomatous pituitary cells . -
pubmed
Lipid - induced
insulin resistance is associated with intracellular accumulation of inhibitory intermediates depending on the prevalent
fatty acid (
FA ) species . -
pubmed
In cultured myotubes ,
ceramide and
phosphatidic acid (
PA ) mediate the effects of the
saturated FA palmitate and the unsaturated
FA linoleate , respectively . -
pubmed
Saline ,
LSF ( 25 mg / kg x d ) , or
MYR ( 0.3 mg / kg x d ) were administered by mini - pumps in the final 4 wk . -
pubmed
Muscle
ceramide and
PA were analyzed by mass spectrometry . -
pubmed
The discrepancy between
ceramide and
glucose tolerance suggests these improvements may not be related directly to changes in muscle
ceramide and may involve other
insulin - responsive tissues . -
pubmed
Changes in the expression of
LASS1 were , however , inversely correlated with alterations in
glucose tolerance . -
pubmed
To date , the large , phase III randomized controlled
breast cancer prevention trials have tested and are testing only hormonal drugs designed to antagonize the carcinogenic effect of endogenous
estrogen ; these agents are either selective
estrogen receptor modulators ( SERMs ) or
aromatase inhibitors ( AIs ) . -
pubmed
Next we demonstrate how this brain
clock controls plasma
glucose concentrations , via projections to sympathetic and parasympathetic pre - autonomic neurons within the hypothalamus . -
pubmed
In agreement with the fact that brain
aromatase activity is correlated with sex
steroid levels , the high expression of
cyp19a1b is due to an autoregulatory loop through which
estrogens and aromatizable
androgens upregulate
aromatase expression . -
pubmed
Here , we identify a previously uncharacterized phosphorylation site on
Ect2 ,
threonine 328 , that serves to regulate the oncogenic activity of
Ect2 in
NSCLC cells . -
pubmed
PKCiota directly phosphorylates
Ect2 at
Thr-328 in vitro , and RNAi - mediated knockdown of either
PKCiota or
Par6 leads to a decrease in
phospho - Thr-328
Ect2 , indicating that
PKCiota regulates Thr-328
Ect2 phosphorylation in
NSCLC cells . -
pubmed
Rapamycin induced apoptosis as well as the expression of
p21 ( waf1 ) mRNA and protein , independent of
p53 . -
pubmed
Rapamycin treatment also resulted in : a decrease in
cdk2 kinase activity ; an increase in hypophosphorylated
retinoblastoma protein (
pRb ) ; a dephosphorylation of
p70 S6 kinase ; and , growth - arrest in G(1)-phase of cell cycle . -
pubmed
These data suggest that
rapamycin - induced growth arrest and apoptosis occur through the
p53 - independent induction of
p21 ( waf1 ) . -
pubmed
Aspirin is an organic anion and could be a substrate for
MRP4 . -
pubmed
RESULTS : Inhibition of
MRP4 - mediated transport by
dipyridamole or
Mk-571 increases
aspirin entrapment and its in vitro effect on
COX-1 activity ( 142.7 + - 34.6 pg/10(8 ) cells vs. 343.7 + - 169.3 pg/108 cells
TxB2 - production ) . -
pubmed
Platelets derived from megakaryocytes transfected with
MRP4 small interfering ribonucleic acid have a higher
aspirin entrapment and drug
COX-1 activity . -
pubmed
CONCLUSIONS :
Aspirin is a substrate for
MRP4 and can be extruded from platelet through its transportation . -
pubmed
ATP - stimulated leukemic cells , but not normal
CD34 + cells , undergo down - regulation of genes involved in cell proliferation and migration , whereas cell - cycle inhibitors are up - regulated . -
pubmed
P2R - expression analysis and pharmacologic profiling suggested that the inhibition of proliferation by
ATP was mediated by the down - regulation of the
P2X7R , which is up - regulated on untreated blasts , whereas the inhibition of chemotaxis was mainly mediated via
P2Y2R and
P2Y4R subtypes . -
pubmed
coli
strains that were improved by an elongation cycle of
fatty acid synthesis (
FAS ) . -
pubmed
The
beta - oxoacyl - ACP synthase enzyme catalyzed the addition of
acyl - ACP to
malonyl - ACP to generate
beta - oxoacyl - ACP . -
pubmed
The enzyme encoded by the
fabG gene converted
beta - oxoacyl - ACP to
beta - hydroxyacyl - ACP , the
fabZ catalyzed the
dehydration of
beta-3 - hydroxyacyl - ACP to
trans-2 - acyl - ACP , and the
fabI gene converted
trans-2 - acyl - ACP to
acyl - ACP for long - chain
fatty acids . -
pubmed
The wild - type
PV ( WT ) as well as three mutant expression constructs were engineered :
lysine 8 to
asparagine ( K8N ) ,
lysine 26 to
asparagine ( K26N ) and a double mutant ( DM ) . -
pubmed
Calcium equilibrium dissociation constants ( K(d ) ) versus temperature curves for all mutants were right - shifted , as predicted , relative to that of WT
PV . -
pubmed
Calculated rates of
Ca ( 2 + ) binding ( k(on ) ) for the K8N and K26N mutants were similar to values for the WT
PV at all temperatures . -
pubmed
In contrast , the calculated k(on ) values for the DM PV were faster , providing mechanistic insights into the nature of potentially adaptive changes in
Ca ( 2 + ) binding in this
PV . -
pubmed
LC -
HR /
MS IN CLINICAL TOXICOLOGY : LC -
HR /
MS has been used to detect designer drugs , doping agents , (
neurosteroids ) and adulterants such as
levamisole , a veterinary antihelmitic found in street
cocaine , and pharmaceuticals in herbal medications marketed to contain only natural ingredients . -
pubmed
The identification of novel designer
amines , stimulants found in " bath salts " , and synthetic
cannabinoids are well suited to LC -
HR /
MS . -
pubmed
MetS was defined as the presence of three or more of the following : blood pressure>=130/90 mm Hg ;
triglycerides>=150 mg / dL ;
high - density lipoprotein<=50 mg / dL for women and & lt;=40 mg / dL for men ; body mass index>=30 kg / m2 ; or fasting blood
glucose>=110 mg / dL. -
pubmed
The distribution of
MetS factors among all patients was
hypertension in 98 % ,
diabetes in 58 % , elevated
triclyceride in 39 % , decreased
high - density lipoprotein in 60 % , elevated
body mass index in 36 % , and 39 % were currently receiving hemodialysis . -
pubmed
COMT , for example , encodes
catechol - o - methyl transferase the enzyme primarily responsible for catabolizing
dopamine in the prefrontal cortex . -
pubmed
Striatal
dopamine function , linked with cognitive tasks as well as habit learning , is influenced by the Taq - Ia polymorphism of the
DRD2 /
ANKK1 gene complex ; this gene influences the density of
dopamine receptors in the striatum . -
pubmed
Participants were genotyped for
COMT (
Val(158)Met ) and
DRD2 /
ANKK1 - Taq - Ia (
A1 + , A1- ) polymorphisms . -
pubmed
After
CD3 + cell depletion , lung homogenates were re - stimulated 24 h with
LPS ( 100 ng / ml ) , PG ( 10 mug / ml ) ,
IL-23 ( 100 ng / ml ) or vehicle . -
pubmed
Therefore , the objective of this experiment was to evaluate the interactions between forage particle size (
FPS ) and ruminally fermentable
carbohydrates (
RFC ) for dry matter intake ( DMI ) , ration sorting , ruminal fermentation , chewing activity , and milk yield and components . -
pubmed
Increasing
RFC was found to increase milk yield , milk protein content and yield , and
lactose content and yield but decrease milk fat content . -
pubmed
Progression - free survival was significantly better with
docetaxel than with
erlotinib : median progression - free survival was 2 9 months ( 95 % CI 2 4 - 3 8) with
docetaxel versus 2 4 months (
2 1 - 2 6 ) with
erlotinib ( adjusted HR 0 71 , 95 % CI 0 53 - 0 95 ; p=0 02 ) . -
pubmed
Transmission electron microscopy (
TEM ) and powder X - ray diffraction ( XRD ) evidenced the formation of
pHA NCs and of their crystalline nature . -
pubmed
The formation of
Au nanoshell -- surrounding pHA
NC -- was confirmed from its surface plasmon resonance (
SPR ) band in the UV / Vis spectrum and by
TEM measurements . -
pubmed
The dissolution of
Au shell was marked by a gradual diminishing of its
SPR band , while the release of pHA molecules in the solution was confirmed from
TEM and FTIR studies . -
pubmed
Synthetic
retinoid Am80 up - regulates
apelin expression by promoting interaction of
RARalpha with
KLF5 and
Sp1 in vascular smooth muscle cells . -
pubmed
Previous studies have demonstrated that both
retinoids and
apelin possess potent cardiovascular properties and that
retinoids can mediate the expression of many genes in the cardiovascular system . -
pubmed
In the present study , we investigated the molecular mechanism of
apelin expression regulation by the synthetic
retinoid Am80 in VSMCs . -
pubmed
The results showed that
Am80 markedly up - regulated
apelin mRNA and protein levels in VSMCs . -
pubmed
Furthermore ,
KLF5 (
Kruppel - like factor 5 ) and
Sp1 (
stimulating protein-1 ) co - operatively mediated
Am80 - induced
apelin expression through their direct binding to the TCE (
transforming growth factor - beta control element ) on the
apelin promoter . -
pubmed
Interestingly , upon
Am80 stimulation , the
RARalpha (
retinoic acid receptor alpha ) was recruited to the
apelin promoter by interacting with
KLF5 and
Sp1 prebound to the TCE site of the
apelin promoter to form a transcriptional activation complex , subsequently leading to the up - regulation of
apelin expression in VSMCs . -
pubmed
Thus the results of the present study describe a novel mechanism of
apelin regulation by
Am80 and further expand the network of
RARalpha in the
retinoid pathway . -
pubmed
Regarding
bezafibrate - associated
rhabdomyolysis and
cibenzoline - associated
hypoglycemia , these
ADR were not detected in cases involving monotherapy . -
pubmed
A
tryptophan metabolite ,
kynurenine , promotes mast cell activation through
aryl hydrocarbon receptor . -
pubmed
BACKGROUND :
Tryptophan metabolites have been suggested to play a role in immune modulation , wherein those have recently been shown to be endogenous ligands of
aryl hydrocarbon receptor (
AhR ; a unique cellular chemical sensor ) . -
pubmed
However , the involvement of
tryptophan metabolites and
AhR in modulating mast cell function remains to be fully defined . -
pubmed
RESULTS :
Kynurenine , but not KA and QA , enhanced
IgE - mediated responses , including degranulation , LTC4 release , and
IL-13 production in BMMCs through the activation of
PLCgamma1 ,
Akt ,
MAPK p38 , and the increase of intracellular
calcium . -
pubmed
These enhancing effects of
KYN were not observed in
AhR - deficient BMMCs and could be inhibited by
CH223191 in BMMCs . -
pubmed
CONCLUSION : The
AhR -
KYN axis is potentially important in modulating mast cell responses and represents an example of
AhR 's critical involvement in the regulation of allergic responses . -
pubmed
In vivo ,
PDE4B deletion could attenuate the lung water content , histological signs of
pulmonary injury and elevate the ratio of partial pressure of arterial
O2 to fraction of inspired
O2 ( PaO2 / FIO2 ratio ) . -
pubmed
Treatment with a
TGFbeta receptor inhibitor ,
galunisertib , reduced the number of arrested cells in brain to 808 + - 82 cells / brain . -
pubmed
Furthermore , we observed a reduction in the percentage of extravasated cells ( from 63 % to 30 % ) compared with cells remaining intralumenal when
TGFbeta is knocked down or inhibited with
galunisertib ( 40 % ) . -
pubmed
Additionally ,
K (+ ) channel blockers , such as
tetraethylammonium (
TEA ) ,
apamin (
APA ) , and
glibenclamide (
Glib ) , had no effect . -
pubmed
However ,
N ( G )- nitro - L - arginine methyl ester (
L - NAME ) and
1H -( 1,2,4 ) oxadiazolo ( 4,3 - A ) quinoxalin-1 - one (
ODQ ) , an inhibitor of
soluble guanylate cyclase (
sGC ) , did inhibit
histamine - induced tonic relaxation . -
pubmed
Ranitidine , the
H2 receptor blocker , inhibited
histamine - induced tonic relaxation . -
pubmed
miRNAs are small noncoding RNAs of approximately 22
nucleotides length , which have emerged as important regulators of genes at
post - transcriptional levels in a range of organisms . -
pubmed
Although
quinazolines are well known to inhibit
EGFR , there is also a large panel of other therapeutic protein targets . -
pubmed
EXPERT OPINION : Since 2011 , a lot of
quinazoline compounds have shown
EGFR inhibition . -
pubmed
Therefore , several patents have been approved for the use of
quinazoline compounds as inhibitors of other
kinases ,
histone deacetylase ,
Nox and some metabolic pathways . -
pubmed
Total
vanadium was determined by inductively coupled plasma - mass spectrometry ( ICP -
MS ) and speciation by ion chromatography - ICP -
MS ( IC - ICP -
MS ) . -
pubmed
Intravenous (
IV )
iron treatment was determined by HCPCS codes , prescription oral
iron by NDC numbers , and blood transfusions by
CPT and ICD-9 - CM codes . -
pubmed
Although most bariatric surgery patients who develop
IDA are tested for
iron , most are not treated with
IV iron or oral
iron and do not receive blood transfusions . -
pubmed
Inhibition of
PKC - delta activation with
rottlerin significantly increased the survival rates and decreased serum
ALT /
AST levels at 6 , 12 and 24 h compared with the control group ( P & lt ; 0.001 ) . -
pubmed
Rottlerin treatment also significantly decreased serum levels of
HMGB1 at 6 , 12 , and 24 h ,
TNF - alpha ,
IL-6 and
IL-1 beta at 12 h compared with the control group ( P & lt ; 0.01 ) . -
pubmed
[ Overexpressing
3 - ketosteroid - Delta1 - dehydrogenase for degrading
phytosterols into
androst-1,4 - diene-3,17 - dione ] . -
pubmed
Depolarization of mitochondria in neurons promotes activation of
nitric oxide synthase and generation of
nitric oxide . -
pubmed
We examined for the first time the effects of mitochondrial depolarization on mitochondrial function , intracellular
calcium ,
neuronal nitric oxide synthase (
nNOS ) activation , and
nitric oxide (
NO ) production in cultured neurons and perivascular nerves . -
pubmed
In addition ,
diazoxide and
BMS not only increased the
nNOS phosphorylation at positive regulatory
serine 1417 but also decreased
nNOS phosphorylation at negative regulatory
serine 847 . -
pubmed
Furthermore ,
diazoxide and
BMS increased
NO production in cultured neurons measured with both fluorescence microscopy and electron spin resonance spectroscopy , which was sensitive to inhibition by the selective
nNOS inhibitor
7 - nitroindazole (
7 - NI ) . -
pubmed
Diazoxide also protected cultured neurons against
oxygen -
glucose deprivation , which was blocked by
NOS inhibition and rescued by
NO donors . -
pubmed
Thus pharmacological depolarization of mitochondria promotes activation of
nNOS leading to generation of
NO in cultured neurons and endothelium - denuded arteries . -
pubmed
The cardioprotective effect of ischemic postconditioning was eliminated after inhibition of
protein kinase C with
cheleritrin or after blockade of delta - isoform of
protein kinase C with
rottlerin . -
pubmed
alternans essential oil by
GC and
GC -
MS and evaluated its antimicrobial and antioxidant activity by disc diffusion ,
DPPH ,
ABTS and FRAP methods , respectively . -
pubmed
Here we have considered the reaction mechanistic chemistry of
epoxides and combined published experimental kinetic data ( rate constants k for reaction with a
cysteine - based peptide ) together with calculated hydrophobicity data ( logP ) to derive a QMM correlating potency in the local lymph node assay ( LLNA ) , expressed as EC3 , with a relative alkylation index (
RAI , calculated as logk + 0.4 logP ) . -
pubmed
The
epoxide QMM predicts the potency of a
nonepoxide SN2 electrophile ( predicted EC3 , 0.48 % ; observed EC3 , 0.5 % ) , which suggests that it could form the basis for a more general H - polar
SN2 QMM that could be a valuable tool in skin sensitization risk assessment for this quite extensive and structurally diverse reaction mechanistic domain . -
pubmed
The putative molecular target of IDPP is
glycoprotein E1 , as revealed by selection for viral drug resistance (
Gly-257 - Arg ) . -
pubmed
CEA was performed at 30 d after craniotomy , comparing the following strategies : mechanical prophylaxis ( MP ) ,
low molecular weight heparin with MP ( MP+LMWH ) , and
unfractionated heparin with
MP ( MP+UFH ) to prevent symptomatic
VTE . -
pubmed
Single
CpG site methylation controls
estrogen receptor gene transcription and correlates with hormone therapy resistance . -
pubmed
In addition ,
luciferase reporter assays showed reduced transcriptional activity from this methylated
CpG site . -
pubmed
This
CpG region was also completely conserved among species , suggesting that it acts as a methylation - sensitive
Ets-2 transcription factor binding site , as confirmed using chromatin immunoprecipitation assays . -
pubmed
In
estrogen receptor alpha - positive tumors , CpG4 methylation levels were inversely correlated with
estrogen receptor alpha expression status , suggesting that single
CpG site plays an important role in the regulation of
estrogen receptor alpha transcription . -
pubmed
jirovecii that could evolve towards
PCP , raising the issue of
cotrimoxazole prophylaxis for at - risk quantitative
polymerase chain reaction ( qPCR)-positive immunocompromised patients . -
pubmed
Here we show that
metformin promotes global DNA methylation in non - cancerous ,
cancer - prone and
metastatic cancer cells by decreasing
S - adenosylhomocysteine (
SAH ) , a strong feedback inhibitor of
S - adenosylmethionine ( SAM )- dependent DNA methyltransferases , while promoting the accumulation of
SAM , the universal
methyl donor for cellular methylation . -
pubmed
Using
metformin and a mitochondria / complex I ( mCI)-targeted analog of
metformin (
norMitoMet ) in experimental pairs of wild - type and
AMP - activated protein kinase (
AMPK)- ,
serine hydroxymethyltransferase 2 (
SHMT2)- and
mCI - null cells , we provide evidence that
metformin increases the SAM :
SAH ratio - related methylation capacity by targeting the coupling between
serine mitochondrial one -
carbon flux and CI activity . -
pubmed
By increasing the contribution of one -
carbon units to the SAM from
folate stores while decreasing SAH in response to
AMPK - sensed energetic crisis ,
metformin can operate as a metabolo - epigenetic regulator capable of reprogramming one of the key conduits linking cellular metabolism to the DNA methylation machinery . -
pubmed
CONCLUSION : Simultaneous inhibition of
EGFR and
IGF1R in
cisplatin - resistant
TNBC cell lines was synergistic regarding inhibition of proliferation and induction of apoptosis . -
pubmed
This assay , together with molecular modeling studies allowed us to select compound
zr17 - 2 for an in vivo experiment , which showed an interesting increase of
CIRBP expression in several organs of experimental animals . -
pubmed
Ferredoxin - dependent bilin reductases (
FDBRs ) are a class of enzymes reducing the
heme metabolite
biliverdin IXalpha (
BV ) to form open - chain
tetrapyrroles used for light - perception and light - harvesting in photosynthetic organisms . -
pubmed
Phylogenetically ,
PcyA is the closest relative catalysing the reduction of BV to
phycocyanobilin . -
pubmed
Biochemical data in combination with the
PcyX crystal structure revealed that a conserved
aspartate -
histidine pair is critical for activity . -
pubmed
Interestingly , the same residues are part of a catalytic
Asp - His - Glu triad in
PcyA , including an additional
Glu . -
pubmed
While this
Glu residue is replaced by
Asp in
PcyX , it is not involved in catalysis . -
pubmed
Substitution back to a
Glu failed to convert
PcyX to a
PcyA. -
pubmed
Multi - facet implications of PEGylated
lysozyme stabilized -
silver nanoclusters loaded recombinant
PTEN cargo in
cancer theranostics . -
pubmed
Herein , we report successful assembly of therapeutically significant
phosphatase protein
PTEN and fluorescent
lysozyme - stabilized
silver nanoclusters to accomplish delivery and tracking of the protein . -
pubmed
The integration of purified recombinant
PTEN with
silver nanoclusters was evaluated by fluorescence spectroscopy study . -
pubmed
Prior to cellular application , the functional integrity of
PTEN in the composite was determined in vitro , by enzymatic assay employing
para - nitrophenylphosphate as substrate . -
pubmed
Thus ,
PEG coated
GST -
PTEN loaded
silver nanoclusters serves as a comprehensive system encompassing cellular imaging and protein delivery with potential biomedical implications . -
pubmed
Eighteen organic contaminants were detected in groundwater in the
PRD , where the most frequently detected organic contaminant was
naphthalene , and its detection rate was 2.51 % . -
pubmed
Anthracyclines ,
paclitaxel ,
carboplatin , and
docetaxel were the
CT agents producing the highest
taste disturbance rates . -
pubmed
In concordance with the metabolome data , metagenomics prediction analysis based on
16S rRNA gene sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with
indole alkaloid biosynthesis ,
cytochrome P450 and intestinal homeostasis , which coincided with up - regulation of
cytokine interleukin-22 and enhanced activation of
aryl hydrocarbon receptor in the recipient colon . -
pubmed
Community - dwelling people with
dementia treated with
warfarin had poorer anticoagulation control than those without
dementia ( mean time in therapeutic range (
TTR ) % + -SD , 38 + -26 (
dementia ) , 61 + -27 ( no
dementia ) , p & lt ; 0.0001 ) . -
pubmed
Synthesis and insecticidal activity in vitro and vivo of novel
benzenesulfonyl derivatives based on potent target subunit H of
V - ATPase . -
pubmed
Two lead compounds with
benzenesulfonamide were found through virtual screening based on the 3D structure of the subunit H of
V - ATPase in previous study . -
pubmed
Finally ,
carnosine has been shown to exert a multimodal activity including inhibition of protein cross - linking and aggregation of
amyloid - beta and related proteins , free radical generation ,
nitric oxide detoxification , and an anti - inflammatory activity . -
pubmed
brassicae by whole - cell
fatty acid ( WCFA ) measurements and real - time
polymerase chain reaction ( PCR ) was evaluated . -
pubmed
Design , synthesis and biological evaluation of
amide - pyridine derivatives as novel dual - target (
SE ,
CYP51 ) antifungal inhibitors . -
pubmed
Based on the analysis of the
squalene cyclooxygenase (
SE ) and
14alpha - demethylase (
CYP51 ) inhibitors pharmacophore feature and the dual - target active sites , a series of compounds with
amide -
pyridine scaffolds have been designed and synthesized to treat the increasing incidence of drug - resistant
fungal infections . -
pubmed
Urinary total
BPA ( free and conjugated ) concentration was quantified using the HPLC -
MS /
MS . -
pubmed
Participants were further stratified into thyroid autoantibody positive group ( at least one positive ) and thyroid autoantibody negative group ( both negative ) according to the
thyroglobulin antibody (
TGAb ) and
thyroid peroxidase antibody (
TPOAb ) levels , and restricted cubic spline regression was also applied to determine the possible nonlinear relationship between urinary
BPA and TNs . -
pubmed
RESULTS : Compared with women in the first quartile , the odds of
TNs was 72 % ( adjusted OR = 1.72 , 95 % CI : 1.25 to 2.35 ) higher for those in the second quartile , 54 % ( adjusted OR = 1.54 , 95 % CI : 1.12 to 2.12 ) higher for those in the third quartile , and 108 % ( adjusted OR = 2.08 , 95 % CI : 1.50 to 2.90 ) higher for those in the fourth quartile after adjusting for age ,
BMI , education ,
HDL - C ,
LDL - C ,
triglyceride , total
cholesterol , urinary
iodine , TGAb and TPOAb . -
pubmed
All
strains , including
fluoroquinolone - resistant and/or extended - spectrum
beta - lactamase - producing
strains , were multi - drug resistant . -
pubmed
Mechanistically , we found that mitochondrial
CHCHD10 is required for
ATP production , which facilitates
AChR expression and promotes
agrin - induced
AChR clustering . -
pubmed
Importantly ,
ATP could effectively rescue the reduction of
AChR clusters in the
CHCHD10 - ablated muscles . -
pubmed
At 25 C , the increase of non - photochemical quenching (
NPQ ) values in cells exposed to 10.0 ppm
Ni might indicate the onset of thermal dissipation process as a self - protection mechanism against
Ni toxicity . -
pubmed
Romiplostim is a peptibody with
c - MPL agonist activity that stimulates endogenous
thrombopoietin production and leads to promoting the proliferation and differentiation of megakaryocytes in the bone marrow . -
pubmed
Herein , a rationally designed a
Cu ( II )-based metal - organic gel (
Cu -
MOG ) with a nanoporous 3D network structure prepared via a simple one - step mixing method was successfully employed for the removal of cationic dyes . -
pubmed
The
Cu -
MOG exhibited high efficiency , with an adsorption capacity of up to 650.32 mg / g , and rapid adsorption efficiency , with the ability to adsorb 80 % of
Neutral Red within 1 min . -
pubmed
Remarkably , the
Cu -
MOG displayed size - selective adsorption , based on adsorption studies concerning dyes of different sizes as calculated by density functional theory . -
pubmed
Additionally , the adsorption performance of the
Cu -
MOG still maintained removal efficiency of 100 % after three regeneration cycles . -
pubmed
These results suggested that the
Cu -
MOG could be expected to be a promising and competitive candidate to conveniently process wastewater . -
pubmed
Then , we measured the protein and mRNA expression of
stathmin ,
p - stathmin (
Ser16 ,
Ser25 ,
Ser38 , and
Ser63 ) ,
beta - tubulin , and
MAP-1B in the hippocampus , amygdala , and mPFC in the 5 groups by immunohistochemistry , Western blotting , and qRT - PCR . -
pubmed
The trend of the protein expression of
p - stathmin (
Ser25 and
Ser38 ) was opposite of that of
stathmin ; it reached a peak on the 7th day , and then decreased in the hippocampus . -
pubmed
The protein expression of
p - stathmin (
Ser63 ) showed the same trend in the mPFC . -
pubmed
Stathmin in the memory loop , especially in the hippocampus , regulates microtubule structure through its phosphorylation at
Ser25 and
Ser38 and thereby participates in the mediation of fear
memory abnormalities in
PTSD . -
pubmed
Low - dose
microcystin - LR antagonizes
aflatoxin B1 induced
hepatocarcinogenesis through decreasing
cytochrome P450 1A2 expression and
aflatoxin B1 - DNA adduct generation . -
pubmed
These results suggest that when co - exposure with
AFB1 ,
MC - LR might repress hepatocarcinogenicity of
AFB1 , which might be associated with its repression on
AFB1 induced
CYP1A2 upregulation and activation . -
pubmed
Efficient adsorption of certain trace organic chemicals ( TOrCs ) present in secondary treated municipal wastewater treatment plant ( WWTP ) effluents onto granular activated
carbon (
GAC ) has already been demonstrated at lab- and full - scale . -
pubmed
Due to high organic matter concentrations in WWTP effluents ,
GAC filters eventually develop a biofilm and turn into biological activated
carbon filters ( BAC ) , where removal of organic compounds is governed by biodegradation as well as by adsorption . -
pubmed
Capto L is an affinity resin based on
Protein L , which binds to the variable region of
kappa light chain without interfering with the antigen binding site . -
pubmed
In this work , we demonstrated that
Capto L provides a convenient means for separating 3/4 antibody from the
bsAb product . -
pubmed
The molecular hybridization approach was based on the combination of
1 - benzyl-4 - piperidine fragment from the anti -
Alzheimer AChE inhibitor
donepezil ( 1 ) and the
cinnamoyl subunit from
curcumin ( 2 ) , a natural product with remarkable antioxidant , neuroprotective and anti - inflammatory properties , using a
N - acylhydrazone fragment as a spacer subunit . -
pubmed
Similarly , compound 4c was capable to prevent the ROS formation , with indirect antioxidant activity increasing intracellular
GSH levels and the ability to counteract the
neurotoxicity induced by both OAbeta1 - 42 and 3 -
NP . -
pubmed
Results of a two - compartment kinetic model demonstrated that aromatic components of
MBCs dominated slow - sorption mechanisms , whereas the embedded
magnetite further accelerated fast - sorption due to H - bonding . -
pubmed
The degradation of SMT occurred because of persistent free radicals ( PFRs ) on BCs or the inherent redox of
iron minerals on
MBCs . -
pubmed
The results showed that YM preadministration to
H2O2 exposition significatively increased cell adhesion after 3 days as well as proliferation and in situ
ALP detection after 10 and 7 days respectively , when compared to
H2O2 group . -
pubmed
Besides , staining of
OCN and
BSP proteins was less intense and scattered in poor spread cells with cytoskeletal changes in
H2O2 group when compared to control and YM
H2O2 group . -
pubmed
It was found that ( a )
HMP stimulation could be selectively inhibited under conditions where release of
granule enzymes remained unchanged or was enhanced , for example , by reduced
glucose concentration or by
2 - deoxyglucose . -
pubmed
The samples were assayed for
acid phosphatase content by a colorimetric method using
sodium thymolphthalein monophosphate as a substrate and by 2 immunochemical assays developed at our laboratory ( counter immunoelectrophoresis and radioimmunoassay ) . -
pubmed
Thus , the depression of KC antigen presentation , as well as the enhanced capacity of these cells to release
inflammatory mediators (
TNF ,
IL-1 ,
IL-6 and
prostanoids ) which may produce cell and organ dysfunction , could contribute to the host 's enhanced susceptibility to
sepsis following
hemorrhage . -
pubmed
The statistical properties of the bursting
potassium channel from fused Torpedo synaptosomes were studied by using patch -
clamp recording and time series analysis . -
pubmed
When the injection was given at 1030 h ,
ICI 154,129 ( 50 micrograms ) exerted no significant effects on either
LH or
PRL . -
pubmed
As newer agents become available ( eg , beta - blockers with ancillary properties ,
calcium channel blockers with allegedly more selective actions on various vascular beds ) , comparative studies between these agents and
ACE inhibitors old and new are awaited with interest . -
pubmed
Neither
CT - A subunit nor
CT - B subunit alone caused
[ 3H ] AA release . -
pubmed
The effect of
CT on AA metabolism is proposed as a possible mechanism leading to the synthesis of
prostaglandin E and fluid secretion during
cholera . -
pubmed
Monastral blue (
MB ) was used as a vascular marker and was infused into the pulmonary artery catheter either for recirculation at time zero or as an end - experiment ( 60 min ) bolus . -
pubmed
The reconstruction of two - dimensional spatial images with 0.5 - mm resolution using radiofrequency gradients generated by an NMR
coil system rotated about the sample is demonstrated for
23Na . -
pubmed
Binding of
C3 on the surface of infected cells was measured in a
biotin - streptavidin amplified ELISA , and complement - mediated lysis was measured in a
51Cr release assay . -
pubmed
A proton transfer triggered by a ligand interacting with the receptor had been suggested as the initial step in the activation of a receptor for the neurotransmitter
serotonin (
5 - hydroxy - tryptamine ; 5 -
HT ) . -
pubmed
With regard to transepithelial
Na transport , the data suggest that
ADH stimulates transport by increasing the
Na permeability of the apical membranes of granular cells specifically . -
pubmed
Amiodarone is known to affect thyroid homeostasis by competitive inhibition of
5 '- monodeiodinase , which converts
L - thyroxine ( T4 ) to
triiodothyronine ( T3 ) and reverse T3 ( rT3 ) to
3,3 '- diodothyronine (
T2 ) , and also by the direct effects of its high
iodine content ( 37 % by weight ) . -
pubmed
These changes were considered to be compensatory adjustments by the pituitary - thyroid axis to competitive inhibition of
5 '- monodeiodinase by the
amiodarone . -
pubmed
Explants cultured within 2 h of expulsion following
prostaglandin induced termination secreted 3.0 times more
insulin after 20 days of culture than those cultured within 2 - 4 h and 8.1 times more than those cultured more than 4 h
post - termination . -
pubmed
Fresh tissue responded to a high concentration of
glucose ( 19.3 mM ) with a small but significant increase in
insulin secretion . -
pubmed
The addition of 10 mM
theophylline caused a major increase in
insulin release . -
pubmed
Cultured tissue did not respond to
glucose alone but did not show increased
insulin release following stimulation with
glucose ( 22 mM ) together with
theophylline ( 10 mM ) in static incubation . -
pubmed
A pharmacokinetic interaction between
clomipramine and
morphine was excluded ; involvement of change in opiate and
5 HT2 receptors by chronic administration of
clomipramine is discussed . -
pubmed
Antibody studies and
aminoacid analysis indicated that it was
albumin . -
pubmed
Study of electrolytic labeling of
fibrinogen with
131 - iodine by Sephadex G-10 gel filtration . -
pubmed
The predicted 403 -
amino acid preprorenin consists of mature
renin and a 66 - residue
amino - terminal prepropeptide . -
pubmed
The possibility that the spectrum of intermediate two , seen in the course of reaction of
flavoenzyme phenol hydroxylases , may be attributable to
iminol isomers of a
flavin - derived
6 - arylamino-5 - oxo ( 3H,5H ) uracil . -
pubmed
A commonly held view of the mechanism of
flavin mixed - function oxidases is that
enzyme - bound
4a - hydroperoxyflavin (
4a - FIHOOH ) undergoes ring opening to provide a
carbonyl oxide (
IV ) , which , after transferring an
oxene equivalent to substrate , yields a
6 - arylamino-5 - oxo ( 3H,5H )- uracil ( I ) . -
pubmed
Comparison of the spectra of III ( and II ) , taken in solvents of widely differing dielectric constants and in a strongly basic medium , with those of the intermediate(s ) observed to be formed in time between
4a - FlHOOH and
4a - FlHOH has shown that the
enzyme - bound intermediate(s ) does not resemble spectrally I nor its
iminol tautomers . -
pubmed
For
LiF TLD in all the media agreement with experiment is improved . -
pubmed
Both spontaneous and
GC - induced apoptosis were increased by the macromolecular synthesis inhibitors ,
cycloheximide (
CHX ) and
puromycin . -
pubmed
Thimerosal ( 100 - 500 microM ) induced a
DTT - reversible block of partially rundown KATP channels , or channels that underwent complete rundown ; these channels were reactivated with
trypsin ( 1 mg / ml ) . -
pubmed
Here , we investigated the non - ionic surfactant
macrogol-260 - cetylstearylether ( TA 6 ) as an efficient vehicle for the reconstitution of
G - proteins . -
pubmed
Reconstitution efficiency of
G - proteins was recorded by
GTP gamma S - binding . -
pubmed
Consumption of the product baked with a high concentration of
Na - propionate significantly lowered the postprandial blood
glucose and
insulin responses , and significantly prolonged the duration of satiety compared with all other breads . -
pubmed
When subjects consumed the breads baked with sourdough ,
lactic acid and
Na - propionate , their
glucose and
insulin responses were reduced compared with the wholemeal bread alone . -
pubmed
Compared to fasting with fluid and salt replacement , fasting with no fluids was associated with higher ( mean , 95 % confidence interval ) total serum
cholesterol ( 8.1 % , 4.3 - 11.9 % ) ,
HDL cholesterol ( 7.5 % , 1.8 - 13.1 % ) ,
LDL cholesterol ( 10.5 % , 2.2 - 18.8 % ) ,
apolipoprotein A-1 ( 8.9 % , 5.0 - 12.8 % ) , and
apolipoprotein B ( 10.5 % , 5.2 - 15.8 % ) . -
pubmed
Here we report that clonal
insulin - secreting HIT beta - cells contain substantial amounts of endogenous
plasmalogens and express a similar
ASCI - PLA2 activity with the following properties : ( 1 ) Enzymatic activity as well as
glucose - induced
eicosanoid release and
insulin secretion are inhibited by a mechanism - based suicide substrate directed towards
ASCI - PLA2 . -
pubmed
( 2 )
HIT cell
ASCI - PLA2 is selectively activated and protected against thermal denaturation by
ATP . -
pubmed
( 3 ) The magnitude of
ASCI -
PLA2 activation by the nonhydrolyzable
ATP analog
AMP - PCP is similar to that by
ATP . -
pubmed
( 4 ) The
ATP concentrations required to activate
ASCI - PLA2 fall within physiologic ranges in the presence of
Mg2 + . -
pubmed
( 5 )
ADP induces a concentration - dependent attenuation of the activation of
ASCI - PLA2 by
ATP . -
pubmed
HIT cell
ASCI - PLA2 exhibited an apparent isoelectric point of 7.5 on chromatofocusing analysis and was quantitatively adsorbed to an
ATP -
agarose matrix and selectively desorbed from this column by
ATP . -
pubmed
Mono - Q anion - exchange analysis of the active
ATP -
agarose eluant yielded a peak of
ASCI - PLA2 activity associated with a single protein band with an apparent molecular mass of 40 kDa . -
pubmed
beta 3 - AR may thus be involved in the control of lipid metabolism , possibly from fat assimilation in the digestive tract , to
triglyceride storage and mobilization in adipose tissues . -
pubmed
Both anti - GalCer and monoclonal antibody
A2B5 , which binds
polysialogangliosides , localized to mitochondria . -
pubmed
The weak adsorption of the linear
proinsulin and fusion protein in non - denaturing conditions , and dynamics of the
SDS -
protein complex 's formation in denaturing conditions were studied . -
pubmed
AcSDKP inhibited the entry of progenitor cells into S phase as measured by
3H - thymidine suicide assay and the absolute number of progenitor cells with peak activity at 10 - 12 M with less activity seen at higher or lower concentrations . -
pubmed
NOEs indicative of multiple and transient beta - turns around the
Asn6 glycosylation site and NOEs fitting X - ray data on a linear V3 peptide -
Fab complex also emerge . -
pubmed
During
CAP , the respiratory muscles
O2 consumption might have accounted for the difference in VO2 consumption between
hypoxia and normoxia . -
pubmed
To optimize vascularization and repair of connective tissue ,
collagen solubility and
glutaraldehyde crosslinking were evaluated . -
pubmed
Data analysis shows that crosslinking of
collagen implants with
glutaraldehyde is associated ( p & lt ; 0.001 ) with detection of the implant . -
pubmed
These results suggest that
glutaraldehyde crosslinking of
collagen implants decreases the rate of biodegradation . -
pubmed
Long - term treatment with
diazepam , a full allosteric modulator of the
GABA ( A ) receptor , results in tolerance to its anticonvulsant effects , whereas an equipotent treatment with the partial allosteric modulator
imidazenil does not produce tolerance . -
pubmed
In this study , tolerance to
diazepam was associated with a selective decrease ( 37 % ) in the expression of the alpha1 subunit in layers III -
IV of the frontoparietal motor cortex , and a concomitant increase in the expression of the alpha5 ( 150 % ) , gamma(2L&S ) and beta(2/3 ) subunits ( 48 % ) ; an increase in alpha5 subunits was measured in all cortical layers . -
pubmed
The results of this study suggest that tolerance to
benzodiazepines may be associated with select changes in subunit abundance , leading to the expression of different
GABA ( A ) receptor subtypes in specific brain areas . -
pubmed
Protein kinases activated by
sphingosine or
N , N '- dimethylsphingosine , but not by other
lipids , have been detected and are termed
sphingosine - dependent protein kinases (
SDKs ) . -
pubmed
SDK1 is a
serine kinase with molecular mass 50 - 60 kDa that is strongly activated by
N , N '- dimethylsphingosine and
sphingosine , but not by
ceramide ,
sphingosine 1 - phosphate , or other sphingo- ,
phospho- , or
glycerolipids tested . -
pubmed
Its activity is inhibited by the
protein kinase C activator
phosphatidylserine . -
pubmed
The phosphorylation site was identified as
Ser * in the sequence
Arg - Arg - Ser - Ser *- Trp - Arg in
14 - 3 - 3 beta . -
pubmed
The sigma and
tau isoforms of
14 - 3 - 3 lack
serine at this position , potentially explaining their lack of phosphorylation by
SDK1 . -
pubmed
Mutation of a
lysine residue , located 9
amino acids N - terminal to the phosphorylation site , abolished
14 - 3 - 3 phosphorylation . -
pubmed
The membrane form of the
CD14 fusion gene , designated
M14 M , contained the full - length
CD14 cDNA sequence , whereas the soluble form of the fusion gene , designated
M14S , was truncated to lack the sequence for the
phosphatidylinositol - anchoring site . -
pubmed
Spontaneous deamidation and isomerization of
Asn108 in prion peptide 106 - 126 and in full - length
prion protein . -
pubmed
On aging , 0.8 mol of
isoaspartyl residue per mole of protein was detected by the
protein - l - isoaspartyl methyltransferase assay ( t(1/2 ) approximately 30 days ) . -
pubmed
Cytochrome c release and
caspase activation during
menadione - induced apoptosis in plants . -
pubmed
Western blot analysis indicated that the
caspase specific inhibitors
AC - DEVD - CHO (
Ac - Asp - Glu - Val - Asp - aldehyde ) and
AC - YVAD - CHO (
N - acetyl - Try - Val - Ala - aspartinal ) inhibited the degradation of a
caspase 3 specific substrate
PARP (
poly ( ADP - ribose ) polymerase ) , and they had no effect on the release of
cytochrome c. -
pubmed
The fate of a
dodecathymidine prodrug in cell extract was monitored by MALDI -
TOF MS . -
pubmed
The
oligonucleotide prodrug was fully metabolized to yield the
dodecathymidine phosphorothioate likely through a
carboxyesterase mediated mechanism . -
pubmed
CONCLUSION : Comparison of these results with those of other groups suggests that hormonal substitution with
insulin and
triiodothyronine in the presence of
myocardial dysfunction could be of potential interest to correct
myocardial dysfunction and increase the number of donor hearts . -
pubmed
After six months of treatment with
carteolol 2 % the
MS increased significantly from 22.4 + /- 2.5 dB to 24.1 + /- 1.8 dB ( p=0.018 ) , and the mean defect ( MD ) fell from 5.3 + /- 0.8 dB to 4.7 + /- 0.6 dB ( p=0.011 ) . -
pubmed
There was no significant difference in the resistance index of the CA , the OA and the
CRA with the two treatments , whereas the resistance index of the
SPCA dropped significantly , from 0.80 + /- 0.05 to 0.77 + /- 0.02 ( p = 0.017 ) . -
pubmed
CONCLUSIONS : CDI did not show significant differences in the resistance indexes of the internal CA , the OA , and the
CRA after treatment with
carteolol 2 % but the resistance index of the
SPCA was significantly reduced . -
pubmed
Carteolol 2 % induced significant changes in the perimetric indexes examined , with an increase in
MS and a decrease in MD . -
pubmed
Liver acid lipase activity was lowered , while alkaline and
lipoprotein lipase activities were elevated due to treatment of single dose of
CCl4 . -
pubmed
On the contrary alkaline , lipoprotein and
hormone sensitive lipases were enhanced after 24 hr of administration of
CCl4 . -
pubmed
Acid lipase activity was raised by administration of different doses of abhrak bhasma concurrent with
CCl4 . -
pubmed
Abhrak bhasma treatment along with
CCl4 enhanced
alkaline lipase activity at 10 and 20 mg dose and later it was reduced at 30 and 40 mg doses and came to normal levels . -
pubmed
When compared with the nonathletes , the runners had lower concentrations of plasma
zinc ( p = 0.012 ) ,
CD3 + ( p = 0.042 ) and
CD19 + lymphocytes ( p = 0.010 ) over the four weeks . -
pubmed
Plasma
zinc concentration correlated with total leucocyte counts in the athletes at
T6 ( r = -0.72 , p & lt ; 0.05 ) and with Pokeweed mitogen stimulation in the nonathletes at
T6 ( r = -0.92 , p & lt ; 0.05 ) . -
pubmed
The active site region of
NTE as a recombinant protein preferentially hydrolyzes
lysolecithin , suggesting that this enzyme may be a type of
lysophospholipase (
LysoPLA ) with
lysolecithin as its physiological substrate . -
pubmed
The findings indicate that tissue
glutathione - dependent enzymes as well as other antioxidant enzymes function in protection against
Cd2 + toxicity and that these antioxidants provide a first line of defense against
Cd2 + before the induction of any
metallothionein synthesis occurs . -
pubmed
Soil pH had no significant effect on soil total
Zn toxicity thresholds , whereas significant positive correlations were found between these thresholds and background
Zn for the
PNR and SIR test ( r = 0.74 and 0.71 , respectively ; log - log correlations ) . -
pubmed
We first develop appropriate parameters to describe models of the redox site of
rubredoxins , followed by some preliminary calculations of multinuclear
iron systems of relevance to
hydrogenases . -
pubmed
Measurement of
MMP for viable cells at the end of the exposure revealed a decrease in
Rhodamine 123 uptake , which indicates a loss of
MMP . -
pubmed
Plasma was precipitated with
acetonitrile before samples were loaded onto a zwitterionic hydrophilic interaction liquid chromatography (
ZIC - HILIC ) solid - phase extraction column . -
pubmed
Improved flap viability with site - specific delivery of
sildenafil citrate using
fibrin glue . -
pubmed
Sildenafil is a cyclic
guanosine - specific
phosphodiesterase type 5 (
PD-5 ) inhibitor that is widely used for
erectile dysfunction . -
pubmed
Potent and competitive inhibition of
PD-5 enhances levels of
cyclic guanosine monophosphate (
cGMP ) . -
pubmed
In this study , local delivery of
Sildenafil citrate with
fibrin glue was accomplished to improve random flap survival . -
pubmed
In Group
IV ( n = 10 ) , 2.5 mg of
sildenafil citrate mixed in 0.5 mL of
fibrin glue was applied to donor site of the flap , whereas 10 mg of
sildenafil citrate mixed in 0.5 mL of
fibrin glue was applied in Group V ( n = 10 ) . -
pubmed
Sildenafil and
fibrin glue groups ( Group
IV and V ) resulted in a statistically significant decrease in flap
necrosis compared with Groups
II and III ( P & lt ; 0.0001 ) . -
pubmed
It acetylates the
COX enzyme , irreversibly inhibiting the formation of
prostaglandin . -
pubmed
Cytosol , depleted of
ATP - binding proteins , did not support the sufficient nuclear export of
importin beta . -
pubmed
The high - resolution mapping of the right atrium using a noncontact mapping system during AFL and pacing from the coronary
sinus (
CS ) was performed to evaluate the conduction through the
ER . -
pubmed
Type III ( n = 24 , M / F = 20/4 , 61 + /- 16 years ) exhibited an activation wavefront that passed along the
ER , with the
sinus venosa as the posterior barrier during AFL . -
pubmed
The
Fc - labeled dNTPs were good substrates for
DNA polymerases and were efficiently incorporated to DNA by primer extension ( PEX ) . -
pubmed
Transketolase is a prominent
thiamin diphosphate - dependent enzyme in
sugar metabolism that catalyzes the reversible transfer of a
2 - carbon dihydroxyethyl fragment between a donor
ketose and an acceptor
aldose . -
pubmed
coli in a covalent complex with donor
ketoses d - xylulose 5 - phosphate (
X5P ) and
d - fructose 6 - phosphate (
F6P ) at 1.47 A and 1.65 A resolution reveal significant
strain in the tetrahedral cofactor -
sugar adducts with a 25 - 30 degrees out - of - plane distortion of the
C2 - Calpha bond connecting the substrates '
carbonyl with the
C2 of the cofactor 's
thiazolium part . -
pubmed
The X - ray structure with the acceptor
aldose d - ribose 5 - phosphate (
R5P ) noncovalently bound in the active site suggests that the
sugar is present in multiple forms : in a strained ring - closed
beta - d - furanose form in
C2 - exo conformation as well as in an extended acyclic
aldehyde form , with the reactive
C1 aldo function held close to
Calpha of the presumably planar
carbanion /
enamine intermediate . -
pubmed
PorB(IA ) triggers efficient adherence and invasion under low
phosphate conditions mimicking
systemic bloodstream infections . -
pubmed
However , it is not known how
p53 aggravates
doxorubicin (
Dox)-induced toxicity in the heart . -
pubmed
Inhibition of
p53 by pifithrin - alpha (
PFT - alpha ) provided different levels of protection from
Dox that correlate with
HSP27 levels in these cells . -
pubmed
In
HSF-1(+/+ ) cells ,
PFT - alpha attenuated
Dox - induced toxicity . -
pubmed
On the other hand , immunoprecipitation of
p53 was found to coimmunoprecipitate
HSP27 and vice versa ( confirmed by Western blotting and matrix - assisted laser desorption / ionization time of flight ) , demonstrating
HSP27 binding to
p53 in
Dox - treated cells . -
pubmed
Further analysis with flow cytometry showed that increased expression of
p21 results in G(2)/M phase cell cycle arrest in
Dox - treated cells . -
pubmed
cTnI ( 24 kDa , pI approximately 9.5 ) contains twelve
serine , eight
threonine , and three
tyrosine residues , which presents a challenge for unequivocal identification of phosphorylation sites and quantification of positional isomers . -
pubmed
Thirty - six molecular ions of
cTnI were detected in a single ESI / FTMS spectrum despite running as a single sharp band on
SDS - PAGE . -
pubmed
Electron capture dissociation of monophosphorylated
cTnI localized two major basal phosphorylation sites : a well known site at
Ser(22 ) and a novel site at
Ser(76)/
Thr(77 ) , each with partial occupancy (
Ser(22 ): 53 % ;
Ser(76)/
Thr(77 ): 36 % ) . -
pubmed
Top down MS(3 ) analysis of diphosphorylated
cTnI revealed occupancy of
Ser(23 ) only in diphosphorylated species consistent with sequential ( or ordered ) phosphorylation / dephosphorylation of the
Ser(22/23 ) pair . -
pubmed
Timely
levodopa (
LD ) administration prolongs survival in
Parkinson ' s disease . -
pubmed
Levodopa (
LD ) is the most effective drug for symptomatic control of
Parkinsonism (
PS ) . -
pubmed
Survival improved significantly coincident with the widespread unrestricted access to
LD ( p = 0.024 - & lt ; 0.001 ) compared to pre -
levodopa era cases . -
pubmed
When the major disease and treatment related factors were considered the timely use of
levodopa in the recent cases resulted in improved survival compared to the earlier cases who received
LD later in the course of illness . -
pubmed
NS-398 suppressed the expression of the
survivin gene in a concentration- and time - dependent manner . -
pubmed
CONCLUSIONS :
Survivin was down - regulated in the growth inhibition of
hepatoma cells induced by a selective
COX-2 inhibitor ,
NS-398 , in a concentration- and time - dependent manner . -
pubmed
This
cAMP - dependent
rpS6 kinase activity is also sensitive to
PKI in vitro , and
PKA exclusively phosphorylates recombinant
rpS6 on
Ser235 /
Ser236 in vitro . -
pubmed
With these data taken together , we conclude that
PKA can phosphorylate
rpS6 exclusively at
Ser235 /
Ser236 in vivo in pancreatic beta - cells , thus providing a potentially important link between
cAMP signalling and the regulation of protein synthesis . -
pubmed
Tetra - and pentasubstituted polyfunctional dihydropyrroles have been concisely synthesized in high yields by two different processes of the one -
pot multicomponent reactions ( MCRs ) of
but-2 - ynedioates 1 ,
amines 2 , and
aldehydes 3 at room temperature or at 70 degrees C. The first one involves a domino hydroamination / nucleophilic addition / amidation - cyclization process and leads to the formation of tetrasubstituted polyfunctional
dihydropyrroles 4 . -
pubmed
While PEGPEI
luciferase expression in vitro was approximately three times higher when compared to
BPEI , a transfection rate at the level of naked DNA was observed in vivo . -
pubmed
The
protein contains 108 haems per molecule and 12
seryl , 12
threonyl , 35
glutamyl and 45
valyl residues to give a total number of 104 residues per molecule as
N - terminal amino acids . -
pubmed
Compared to untreated cells , treatment with BM - ca inhibited both the constitutively activated
NF - kappaB and
p38 MAPK pathways , as assessed by inhibition of both
phospho - p65 and
phospho - IkappaBalpha and
phospho -
p38 , respectively , but not the unphosphorylated forms . -
pubmed
Some emerging
aurora kinase inhibitors , such as
VX-680 ,
PHA-739358 ,
MK-0457 and
AS703569 , and
Smo1 and
Hedgehog (
Hh ) inhibitors promise clinical efficacy against the
Bcr - Ab T315I mutant form and
leukaemia stem cells , respectively . -
pubmed
We suggest that the origin of the emission in
Bi :
GLS is
Bi22- dimers . -
pubmed
Gastrointestinal digestion of
ovalbumin (
OVA ) was simulated using an in vitro system in two steps , which mimicked digestion in the stomach and duodenum , to assess the effect of different gastric pHs , different concentrations of
proteases , and the presence of surfactants , such as
phosphatidylcholine ( PC ) and
bile salts ( BS ) . -
pubmed
Pretreatment with
MAG prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight / dry weight ratio , in total leukocyte number and neutrophil percent in the BALF , and in
myeloperoxidase (
MPO ) activity of lung in dose - dependent manners . -
pubmed
At the same time , pretreatment with
MAG also significantly improved the
super oxide dismutase (
SOD ) activity and induced the
malondialdehyde (
MDA ) content in the bronchoalveolar lavage fluid ( BALF ) . -
pubmed
Importantly , pretreatment with
MAG prevented an increase in
cyclic adenosine monophosphate - phosphodiesterase (
cAMP - PDE ) activity of lung in a dose - dependent manner . -
pubmed
These results showed that anti - inflammatory effects of
MAG against the LPS - induced
ALI may be due to its ability of primary inhibition of
cAMP - PDE activity , oxidative stress and its regulation of
cytokine effects . -
pubmed
Perifosine , a new synthetic
alkylphospholipid Akt inhibitor , has shown anti - tumor activity by inhibition of
Akt phosphorylation . -
pubmed
The
caspase cascade and its downstream effectors ,
Poly ( ADP - ribose ) polymerase (
PARP ) , were also activated simultaneously upon
perifosine treatment . -
pubmed
The proapoptotic effect of
perifosine was in part depending on regulation of the phosphorylation level of
ERK and
JNK . -
pubmed
Importantly , the expression of
dynAP activated
Akt ( also known as
protein kinase B ) by promoting
Ser473 phosphorylation required for the full activation , whereas knockdown of
dynAP abolished this activation . -
pubmed
We now describe the identification of a new
ATM phosphorylation site ,
Thr(P)(1885 ) and an additional autophosphorylation site ,
Ser(P)(2996 ) , that is highly DNA damage - inducible . -
pubmed
We targeted the
ATM phosphorylation sites ,
Ser(367 ) and
Ser(2996 ) , for further study by generating phosphospecific antibodies against these sites and demonstrated that phosphorylation of both was rapidly induced by radiation . -
pubmed
As found for
Ser(P)(1981 ) ,
ATM phosphorylated at
Ser(367 ) and
Ser(2996 ) localized to sites of DNA damage induced by radiation , but
ATM recruitment was not dependent on phosphorylation at these sites . -
pubmed
Phosphorylation at
Ser(367 ) and
Ser(2996 ) was functionally important because mutant forms of
ATM were defective in correcting the S phase checkpoint defect and restoring radioresistance in
ataxia - telangiectasia cells . -
pubmed
PURPOSE : The aim of this study was to investigate the bone response to rough
titanium implants treated with
hydrofluoric acid /
nitric acid (
HF /
HNO3 ) solution . -
pubmed
MATERIALS AND METHODS : Implants were treated with
HF /
HNO3 solution ( test implants ) or without
HF /
HNO3 solution ( control implants ) . -
pubmed
Structures of
1 - Deoxy - D - Xylulose-5 - Phosphate Reductoisomerase / Lipophilic
Phosphonate Complexes . -
pubmed
Fosmidomycin , a potent inhibitor of
1 - deoxy - D - xylulose-5 - phosphate reductoisomerase (
DXR ) , has antibacterial and antimalaria activity . -
pubmed
Here ,
pyridine /
quinoline containing
phosphonates are identified to be
DXR inhibitors with IC(50 ) values as low as 840 nM. -
pubmed
The crystal structure of an N - terminally truncated
chaperonin reveals a monomeric protein with a fully open
nucleotide binding site . -
pubmed
When compared with closed state group
II chaperonin structures , a large - scale 30 rotation between the equatorial and intermediate domains is observed resulting in an open
nucleotide binding site . -
pubmed
A protein preparation obtained from the
Ehrlich ascites carcinoma (
EAC ) by
acetone precipitation , water extraction and subsequent two - step
alcohol fractionation reduced significantly
EAC cell proliferation in vivo and in vitro . -
pubmed
The ancestral allele has largely disappeared from
today 's Pto populations suggesting that
flagellin - triggered immunity limits pathogen fitness even in highly virulent pathogens . -
pubmed
METHODS : Of 83
lamivudine - resistant patients , 49 and 34 patients harboured the
rtM204I and rtM204V mutations , respectively . -
pubmed
EGFR inhibitors (
gefitinib and
AEE788 ) reduced clonogenicity , proliferation and survival of
ependymoma - SC lines dose - dependently , and blocked
EGF - induced activation of
EGFR ,
Akt and
extracellular signal - regulated kinase 1 / 2 . -
pubmed
However , ex vivo treatment with
AEE788 did not impair orthotopic
tumor engraftment , whereas ex vivo differentiation did , suggesting that
CD133 does not absolutely segregate for tumorigenicity in
ependymoma - SCs . -
pubmed
Accordingly ,
Tat decreases substrate oxidation by mitochondria isolated from these tissues , with
oxygen uptake being initially restored by adding
cytochrome c. -
pubmed
The anion - channel inhibitor
4,4 '- diisothiocyanostilbene-2,2 '- disulfonic acid (
DIDS ) protects isolated mitochondria against
Tat - induced mitochondrial membrane permeabilization ( MMP ) , whereas
ruthenium red , a
ryanodine receptor blocker , does not . -
pubmed
NO synthase inhibitor
n - nitro - l - arginine did not modify the relaxing effect provoked by
AMP suggesting that the mechanism of action of this
nucleotide does not involve the
NO pathway . -
pubmed
We recently reported that S - nitrosylation of
Cdk5 ( forming
SNO -
Cdk5 ) at specific
cysteine residues results in excessive activation of
Cdk5 , contributing to
mitochondrial dysfunction ,
synaptic damage , and neuronal cell
death in models of
AD . -
pubmed
Here we report the identification and characterization of noncyclic
nucleotide EPAC antagonists that are exclusively specific for the
EPAC2 isoform . -
pubmed
These
EAPC2 - specific antagonists , designated as
ESI-05 and
ESI-07 , inhibit
Rap1 activation mediated by
EAPC2 , but not
EPAC1 , with high potency in vitro . -
pubmed
Moreover ,
ESI-05 and
ESI-07 are capable of suppressing the
cAMP - mediated activation of
EPAC2 , but not
EPAC1 and
PKA , as monitored in living cells through the use of
EPAC- and
PKA - based FRET reporters , or by the use of
Rap1 -
GTP pull - down assays . -
pubmed
Deuterium exchange mass spectroscopy analysis further reveals that
EPAC2 - specific inhibitors exert their isoform selectivity through a unique mechanism by binding to a previously undescribed allosteric site : the interface of the two
cAMP binding domains , which is not present in the
EPAC1 isoform . -
pubmed
We evaluated
GC sensitivity using 2
cortisol suppression tests : a very - low - dose intravenous
dexamethasone suppression test ( VLD -
IV -
DST ) and a low - dose oral
dexamethasone suppression test . -
pubmed
A positive correlation was found between the extent of
cortisol suppression in vivo ( VLD -
IV -
DST ) and the number of days elapsed since the last flare of lupus activity . -
pubmed
Amurensin G treatment induced punctate cytoplasmic expression of GFP -
LC3 and increased the expression level of endogenous
LC3 - II . -
pubmed
Amurensin G inhibited
rotenone - induced apoptosis and interfered with
rotenone - induced
G2 / M cell cycle arrest . -
pubmed
In addition , knockdown of
beclin1 , a regulator of autophagy , abolished the effect of
amurensin G. -
pubmed
The model predicted the existence of a UV dose threshold for the proper functioning of the
PRR model , and highlighted an overlapping effect of
Nucleotide Excision Repair ( the pathway effectively responsible to clean the genome from UV lesions ) on the dynamics of
PCNA ubiquitylation in different phases of the cell cycle . -
pubmed
A potential role for
substance P and
interleukin-6 in the cerebrospinal fluid of Cavalier King Charles Spaniels with
neuropathic pain . -
pubmed
CONCLUSION : Release of
interleukin-6 and
substance P may initiate proinflammatory effects leading to development of persistent
pain in CKCSs with
syringomyelia . -
pubmed
Expression of
Sproutys and
SPREDs is decreased during lung branching morphogenesis in
nitrofen - induced
pulmonary hypoplasia . -
pubmed
We hypothesized that expression of
Sprouty2 ,
Sprouty4 ,
SPRED-1 and
SPRED-2 is decreased during lung branching morphogenesis in
nitrofen - induced
PH . -
pubmed
Immunoreactivity of
Sprouty2 ,
Sprouty4 ,
SPRED-1 and
SPRED-2 was markedly diminished on E18.5 in
nitrofen - induced PH . -
pubmed
The blood samples were collected punctually after oral administration and a specific gas chromatographic system - flame ionization detector (
GC - FID ) method was developed and employed to determine the level of
borneol in the plasma . -
pubmed
Diagnostic performance of
FDG PET or PET /
CT in prosthetic
infection after arthroplasty : a meta - analysis . -
pubmed
FDG PET or PET /
CT are accurate methods in this setting . -
pubmed
According to the XRD ,
TEM and XPS analysis , partially oxidated
Ag NCs have been formed on the surfaces of the
BET grains . -
pubmed
To our knowledge , however , the effectiveness of drugs for preventing exacerbations of
asthma in the autumn has not been studied previously , except for
leukotriene receptor antagonists and
Omalizmab . -
pubmed
We further examined the involvement of intracellular
calcium ions ( [
Ca2+]i ) in the ES - induced
BDNF production in SCs by pharmacological studies . -
pubmed
We found that the ES - induced
BDNF release required
calcium influx through
T - type voltage - gated calcium channel ( VGCC ) and
calcium mobilization from internal
calcium stores , including
inositol triphosphate - sensitive stores and
caffeine /
ryanodine - sensitive stores . -
pubmed
In conclusion , ES is capable of activating SCs to secrete
BDNF , which requires the involvement of
calcium influx through T - type VGCC and
calcium mobilization from internal
calcium stores . -
pubmed
Liver injury ( transaminase levels ) ,
mitochondrial damage (
glutamate dehydrogenase activity ) oxidative stress (
malondialdehyde levels ) , and
nicotinamide adenine dinucleotide (
NAD (+ ) ) , the co - factor necessary for
SIRT1 activity , were determined by biochemical methods . -
pubmed
SIRT1 and its substrates ( ac -
FoxO1 ,
ac - p53 ) , the precursor of
NAD (+ ) ,
nicotinamide phosphoribosyltransferase (
NAMPT ) , as well as the phosphorylation of
adenosine monophosphate activated protein kinase (
AMPK ) , p -
mTOR , p -
p70S6 K ( direct substrate of
mTOR ) , autophagy parameters (
beclin-1 ,
LC3B ) and
MAP kinases ( p -
p38 and p -
ERK ) were determined by Western blot . -
pubmed
The elevated
SIRT1 activity in presence of
TMZ can be attributed to the enhanced
NAMPT protein and
NAD(+)/
NADH levels . -
pubmed
Furthermore ,
MAP kinases were regulated in livers preserved with
IGL-1 +
TMZ , as they were characterized by enhanced p -
ERK and decreased p -
p38 protein expression . -
pubmed
CONCLUSION : Our study shows that IGL-1 preservation solution enriched with
TMZ protects liver grafts from the
IRI associated with
OLT , through
SIRT1 up - regulation . -
pubmed
When
lysine-9 residues in the peptides were methylated , they were protected from cleavage by
endoproteinase - EndoLysC , whereas unmethylated peptides were cleaved , resulting in an increase in fluorescent intensity . -
pubmed
Trichostatin A (
TSA ) is a
histone deacetylase inhibitor and a potential therapeutic for various
malignancies . -
pubmed
TSA did not affect overall
Grg1 protein levels , but instead reduced
ErbB1 and
ErbB2 expression , which are upregulated by
Grg1 in the absence of
TSA . -
pubmed
We additionally found that
TSA reduced the expression of
VEGF and
VEGFR2 , but not
basic FGF and
FGFR1 . -
pubmed
Our findings indicate that
TSA effectively inhibits
Grg1 - induced lung
tumorigenesis through the down - regulation of
ErbB1 and
ErbB2 , as well as reduced
VEGF signaling . -
pubmed
This suggests
TSA and other
HDAC inhibitors could have therapeutic value in the treatment of
lung cancers with
Grg1 overexpression . -
pubmed
However , effects of chronic
MA exposure or acute
MA exposure following withdrawal on neural activation patterns within brain regions that regulate the
HPA axis are unknown . -
pubmed
RESULTS : ( Experiment 1 ) Compared to exposure to a single dose of
MA ( 5 mg / kg ) , chronic
MA exposure decreased the number of
c - Fos expressing cells in the paraventricular hypothalamus , dorsomedial hypothalamus , central amygdala , basolateral amygdala , bed nucleus of the stria terminalis ( BNST ) , and
CA3 hippocampal region . -
pubmed
CONCLUSIONS :
HPA axis - associated amygdala , extended amygdala , and hippocampal regions endure lasting effects following chronic
MA exposure and therefore may be linked to stress - related
withdrawal symptoms . -
pubmed
OBJECTIVE : The New Delhi
metallo - beta - lactamase-1 (
NDM-1 ) enzyme is a plasmid - encoded enzyme that inactivates
carbapenem antibiotics . -
pubmed
METHODS : Over a period of one year , all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one
carbapenem were subjected to MHT and conventional
polymerase chain reaction ( PCR ) detection of the
NDM-1 gene . -
pubmed
A series of
1 - phenyl-3 / 4 -[ 4 -( aryl / heteroaryl / alkyl - piperazine1 - yl )- phenyl - urea derivatives ( 29 - 42 ) were designed , synthesized and evaluated for their anticonvulsant activity by using maximal electroshock (
MES ) , subcutaneous
pentylenetetrazole (
scPTZ )
seizure tests . -
pubmed
Compound 30 (
1 -{ 4 -[ 4 -( 4 - chloro - phenyl )- piperazin-1 - yl ]- phenyl }- 3 - phenyl - urea ) exhibited marked anticonvulsant activity in
MES as well as scPTZ tests . -
pubmed
The phase
II anticonvulsant quantification study of compound 30 indicates the ED50 value of 28.5 mg / kg against
MES induced
seizures . -
pubmed
Liver X receptors and
copper metabolism : New frontiers for the
oxysterol receptors . -
pubmed
OBJECTIVE : This study aimed to compare the clinical and radiologic outcomes of AO
titanium locking plate and screw ( ATLPS ) and anterograde intramedullary (
AIM ) fixation for treating unstable
metacarpal and phalangeal fractures . -
pubmed
At 3 - month follow - up , all clinical outcomes were significantly better in the
AIM than
ATLPS group ( P & lt ; 0.05 ) except for VAS
pain scores . -
pubmed
This review focused on i ) functional interactions between homologous and heterologous pathways , ii ) methodologies applied for determining receptor endocytosis , iii ) experimental tools to determine specific endocytic routes , iv ) roles of small
guanosine triphosphate - binding proteins in
GPCR endocytosis , and v ) role of
post - translational modification of the receptors in endocytosis . -
pubmed
Basal plasma
cortisol concentrations were higher at
T1 compared with
T2 and T3 ( p & lt ; .05 ) . -
pubmed
The adrenal cortex sensitivity ( expressed as total AUC ( AUCt ) of
cortisol response after
ACTH application ) was lowest at
T2 compared with
T1 and T3 ( p & lt ; .05 ) . -
pubmed
Enhancing the
energy deficiency during early lactation by omission of concentrate did not affect baseline
cortisol concentrations in plasma , but decreased peak height at
T2 ( p & lt ; .05 ) . -
pubmed
Baseline plasma
cortisol concentrations were positively correlated with
cortisol peak values after
ACTH application , previous lactation performance , milk yield and BW ( p & lt ; .05 ) . -
pubmed
Cortisol release after
ACTH injection was lower in animals with high plasma concentrations of
NEFA ,
BHB and with higher contents of fat and
free fatty acids in milk ( p & lt ; .05 ) . -
pubmed
Group
CT was associated with a lower likelihood of being a heavy drinker 3 months later ; Group ST was associated with decreased readiness and confidence to change
alcohol use . -
pubmed
Facilitator speech and peer responses were related to
CT and ST during the group sessions with this high risk population , which were then associated with individual changes for
alcohol use . -
pubmed
So , the purpose of this study was to prepare controlled - release floating (
CRF ) tablets of
dipyridamole by the dry - coated method . -
pubmed
Two
MOF films ,
HKUST-1 and
ZnMOF-74 , are assembled so that the oxidation of one corresponds to the reduction of the other , allowing the two sides of the device to simultaneously change color . -
pubmed
These
MOF films exhibit cycling stability unrivaled by other MOFs and a significant optical contrast in a
lithium - based
electrolyte . -
pubmed
HKUST-1 reversibly changed from
bright blue to light blue and
ZnMOF-74 from yellow to brown . -
pubmed
The electrochromic device associates the two
MOF films via a
PMMA -
lithium based
electrolyte membrane . -
pubmed
Here we show that the
serine /
threonine protein kinase
Sch9 branch of
TORC1 signaling depends specifically on the integrity of the vacuolar membrane , and this dependency originates in changes in
Sch9 localization reflected by
phosphatidylinositol 3,5 - bisphosphate . -
pubmed
In particular , transverse tubules ( T - tubules ) and caveolae have been found to be critical structural components for the spatial confinement of
cAMP in cardiomyocytes , as exemplified by
beta - adrenergic receptor ( & amp;
beta ;- ARs ) signaling . -
pubmed
In addition , we review the role of localized
phosphodiesterase (
PDE ) activity in constraining the
cAMP signal . -
pubmed
High risk includes 1 ) gestational age (
GA ) & lt;=30 weeks , 2 ) birth - weight ( BW ) & lt;=1000 g , 3 )
GA 30 - 32 weeks and BW & lt;=1250 g , 4 ) born from a mother who received
indomethacin for tocolysis . -
pubmed
ERG (
ether - a - go - go - related gene ) channels are the members of the voltage - dependent
potassium channel family , which have three subtypes , as
ERG1 (
Kv 11.1 ) ,
ERG2 (
Kv 11.2 ) , and
ERG3 (
Kv11.3 ) . -
pubmed
For that reason , we aimed to study biophysical properties and physiological importance , and contribution of
ERG K + currents to the formation of action potentials in the stellate and bushy neurons of the ventral cochlear nucleus ( VCN ) . -
pubmed
In current
clamp ,
ERG channel blockers ,
terfenadine ( 10 microM ) and
E-4031 ( 10 microM ) , were applied in both cell types . -
pubmed
When the
lactose transporter (
Lac12 ) , de novo
GDP - l - fucose pathway ( consisting of
GDP - d - mannose-4,6 - dehydratase (
Gmd ) and
GDP-4 - keto-6 - deoxymannose-3,5 - epimerase-4 - reductase (
WcaG ) ) , and
alpha1,2 - fucosyltransferase (
FucT2 ) were introduced , the resulting engineered
strain ( D452L - gwf ) produced 0.51 g / L of
2 - FL from a batch fermentation . -
pubmed
In addition , 0.41 g / L of
l - fucose was produced when
alpha - l - fucosidase was additionally expressed in the 2 - FL producing
strain ( D452L - gwf ) . -
pubmed
Taken together , our study elucidated the importance of
PD - L1 associated with
tumor cells and non -
tumor host cells , particularly BM - derived hematopoietic cells , as well as
PD - L2 inducibly expressed on
TAM in the suppression of anti -
tumor immunity in the
tumor microenvironment . -
pubmed
Dexmedetomidine is a
alpha2 - adrenoreceptor agonist and this drug possesses sedative , anxiolytic , analgesic , and anesthetic - sparing effect . -
pubmed
Immunohistochemistry for
tyrosine hydroxylase (
TH ) ,
5 - hydroxytryptamine (
5 - HT ;
serotonin ) ,
tryptophan hydroxylase (
TPH ) and western blot for
D1 dopamine receptor were also performed . -
pubmed
Dexmedetomidine decreased the immobility latency and increased the expressions of
TPH ,
5 - HT , and
D1 dopamine receptor , whereas ,
HT expression was decreased by
dexmedetomidine treatment . -
pubmed
CONCLUSION : In our results ,
dexmedetomidine alleviated
sleep deprivation - induced
depressive behaviors by increasing
5 - HT synthesis and by decreasing
dopamine production with up - regulation of
D1 dopamine receptor . -
pubmed
GC -
MS analysis revealed that , the
ethyl acetate precipitation contains
pyrrolo ( 1,2 - A ( pyrazine-1,4 - dione ,
hexahydro-3 -( 2 - methylpropyl )- ) and
actinomycin C2 . -
pubmed
BACKGROUND : The
SWEET proteins are a group of
sugar transporters that play a role in
sugar efflux during a range of biological processes , including stress responses . -
pubmed
FGF23 is a hormone that is mainly known as the core regulator of
phosphate and
vitamin D metabolism and it has been recognized as an important regulator of bone mineralization . -
pubmed
In a culture study ,
Epo promoted BMMSC proliferation in normoxia and enhanced cell survival under the culture condition mimicking
ischemia ( 1 %
oxygen and nutrient deprivation ) . -
pubmed
The SL /
PVA /
SA beads showed a network structure with a wide range of macropores . -
pubmed
Compared with blank beads , the SL /
PVA /
SA beads showed an increased rough surface and whole distribution on the surface with the increase of SL . -
pubmed
In addition , SL /
PVA /
SA beads have more uniform pore size , but the porosity of SL /
PVA /
SA beads was decreased with the increase of SL . -
pubmed
The porosity of the beads was 83.24 % , 74.24 % , 71.48 % and 71.29 % for blank beads and SL /
PVA /
SA beads contained 30 % , 40 % and 50 % SL , when it was used as a solid
carbon source for denitrification . -
pubmed
Owing to their good porosity and biocompatibility , SL /
PVA /
SA beads had shorter acclimation time . -
pubmed
After the exhaustion of
carbon sources ,
nitrate removal rate less than 50 % occurred at the 9th , 10th and 11th day for SL /
PVA /
SA beads that contained 30 % , 40 % and 50 % SL , respectively . -
pubmed
None of the 17
CpG sites in its DNA locus was relevant to
HMMR expression . -
pubmed
Binge drinking is a significant problem in adolescent populations , and because of the reciprocal interactions between
ethanol (
EtOH ) consumption and the endocannabinoid ( eCB ) system , we sought to determine if adolescent
EtOH intake altered the localization and function of the
cannabinoid 1 ( CB1 ) receptors in the adult brain . -
pubmed
Histological analyses indicated that adolescent
EtOH intake significantly reduced the
CB1 receptor distribution and proportion of immunopositive excitatory synaptic terminals in the medial DGML . -
pubmed
Conversely , deficits in eCB - eLTD and recognition memory could be rescued by inhibiting
MAGL with
JZL184 . -
pubmed
These findings indicate that repeated exposure to
EtOH during adolescence leads to long - term deficits in
CB1 receptor expression , eCB - eLTD , and reduced recognition memory , but that these functional deficits can be restored by treatments that increase endogenous
2 - arachidonoylglycerol . -
pubmed
Antipsychotic Drug
Trifluoperazine Suppresses
Colorectal Cancer by Inducing G0 /
G1 Arrest and Apoptosis . -
pubmed
In vitro studies showed that
TFP induced G0 /
G1 cell cycle arrest to dramatically inhibit
CRC cell proliferation through downregulating
cyclin - dependent kinase ( CDK ) 2 ,
CDK4 ,
cyclin D1 , and
cyclin E and upregulating
p27 . -
pubmed
Interestingly ,
TFP treatment increased the expression levels of
programmed death-1 ligand 1 (
PD - L1 ) in
CRC cells and
programmed death-1 (
PD-1 ) in
tumor - infiltrating
CD4 + and
CD8 + T cells , implying that the combination of
TFP with an immune checkpoint inhibitor , such as an anti -
PD - L1 or anti -
PD-1 antibody , might have synergistic anticancer effects . -
pubmed
We report the optical and magneto - optical properties of amorphous and crystalline
Co60Fe20B20 films with thicknesses in the range of 10 nm to 20 nm characterized using spectroscopy ellipsometry (
SE ) and magneto - optical Kerr effect ( MOKE ) spectroscopy . -
pubmed
Serum with various concentrations of
retinol was applied to the left and right sides of the
face and treated with ultrasound . -
pubmed
Mild side effects appeared mainly after the first treatment and on the left side of the
face with 0.5 %
retinol . -
pubmed
Certain
SEA domains undergo intramolecular proteolysis involving a nucleophilic attack of a
serine hydroxyl group on the preceding
glycine carbonyl . -
pubmed
Since neither a general base , a general acid , nor an
oxyanion hole could be identified in
MUC1 SEA , it has been suggested that proteolysis is accelerated by a non - planarity of the scissile peptide bond imposed by protein folding . -
pubmed
Structural comparison with uncleaved
SEA domains suggests that the number of residues evolutionarily inserted in the cleaved loop of
MUC1 SEA precludes the formation of a properly
hydrogen - bonded beta turn . -
pubmed
In addition to various targets ,
THC and
CBD are also well - known agonists of
peroxisome proliferator - activated receptor gamma (
PPARgamma ) , a nuclear receptor involved in energy homeostasis and lipid metabolism . -
pubmed
In the search of new pCBs potentially acting as
PPARgamma agonists , we identified
cannabimovone (
CBM ) , a structurally unique
abeo - menthane pCB , as a novel
PPARgamma modulator via a combined computational and experimental approach . -
pubmed
CBM promoted the expression of
PPARgamma target genes regulating the adipocyte differentiation and prevented
palmitate - induced
insulin signaling impairment . -
pubmed
Tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1beta (
IL-1beta ) ,
malondialdehyde (
MDA ) and
prostaglandin E2 (
PGE2 ) levels as well as
reduced glutathione (
GSH ) content were assessed in brain homogenate at the end of the schedule . -
pubmed
PTZ increased
TNF - alpha ,
IL-1beta ,
MDA , and
PGE2 levels meanwhile , it decreased
GSH content . -
pubmed
Administration of
VAL ,
DIC ,
MEL ,
VAL +
MEL and
VAL +
DIC decreased
TNF - alpha ,
IL-1beta ,
MDA , and
PGE2 levels meanwhile , they increased
GSH content in the brain homogenates . -
pubmed
XAV939 , an inhibitor of
Wnt /
beta - catenin signaling pathway , decreased
GOLPH3 overexpression - induced proliferation and enhanced
cisplatin - induced angiogenesis inhibition and apoptosis , which was supported by the changes of
VEGF ,
Bax and
Bcl-2 . -
pubmed
Lung Adenocarcinoma Harboring Concomitant
EGFR Mutations and
BRAF V600E Responds to a Combination of
Osimertinib and
Vemurafenib to Overcome
Osimertinib Resistance . -
pubmed
Further , the presence of redox - active metal ions such as
Cu2 + enhances the aggregation process through chelation with these Abeta42 aggregates as well as generation of
Abeta42 - mediated reactive
oxygen species ( ROS ) . -
pubmed
This molecule consists of a natural metal - chelating tetrapeptide motif of
human serum albumin (
HSA ) , a beta - sheet breaker peptide , and a
sugar moiety for better bioavailability . -
pubmed
Moreover ,
Glupep was also shown to sequester out
Cu2 + from the
Abeta -
Cu2 + complex , reducing the ROS formation and toxicity . -
pubmed
All these exciting features show
Glupep to be a potent inhibitor of
Abeta42 - mediated multifaceted toxicity and a prospective therapeutic lead for
AD . -
pubmed
Materials and Methods : Sanger sequencing and the detection of
circNELL2 level after
RNase R or
actinomycin D treatment were performed to identify the existence of
cirNELL2 in
ESCC cells . -
pubmed
Both
NPC1 and
NPC2 are proteins responsible for the exit of
cholesterol from late endosomes and lysosomes (
LE / LY ) . -
pubmed
Triglyceride levels were also independently associated with
osteocalcin levels in premenopausal women ( beta = -0.004 [ P < 0.013 ] ) . -
pubmed
The odds ratio ( OR ) for
MetS was significantly higher in the lowest quartile than in the highest quartile of serum
osteocalcin levels after adjusting for age ,
alkaline phosphatase ,
uric acid , high sensitivity
C - reactive protein , and
body mass index in all women ( OR , 2.00 ; 95 % confidence interval [ CI ] , 1.49 - 2.68 ) as well as in premenopausal ( OR , 2.23 ; 95 % CI , 1.39 - 3.58 ) and postmenopausal ( OR , 2.01 ; 95 % CI , 1.26 - 3.23 ) subgroups . -
pubmed
Electron microscopic observations revealed that these hyperplastic cells originated from type
II pneumoncytes . -
pubmed
In the group with immediate beginning of the function of the kidney at an average
WIZ of 13 minutes the
creatinine clearance was significantly higher and the incidence of severe
infections in the late phase was lower than in the groups with longer
WIZ and very retarded beginning of the renal function . -
pubmed
Glucose disappearance correlated significantly with
insulin concentration . -
pubmed
When arterial
glucose was 10 mM ,
glucose disappearance during maximal
insulin stimulation was fivefold greater than
glucose disappearance in the absence of
insulin . -
pubmed
In the presence or absence of
insulin ,
glucose disappearance was positively correlated with
glucose concentration up to a
glucose concentration range of 30 to 45 mM. -
pubmed
When
insulin ( 500 muU / ml ) was added at any
glucose concentration ,
glucose disappearance was augmented . -
pubmed
In addition to the effect of
insulin on
glucose uptake by the muscle cell ,
glucose mass action appears to be quantitatively equipotent . -
pubmed
The arterial
oxygen saturation of
hemoglobin ( SaO2 ) measured directly with a multiwavelength spectrophotometer was compared with saturation estimated by
pulse oximetry ( SpO2 ) . -
pubmed
The lysosomotropic agents
chloroquine and
NH4Cl did not affect the increase in
insulin degradation produced by transfection with dIDE , indicating that the lysosomal and
IDE - mediated pathways of
insulin degradation are independent . -
pubmed
This release of inhibition , as indicated by increased yields of base
propenal from DNA scission , is enhanced by
menadione , is inhibited by
superoxide dismutase , and is therefore dependent on
superoxide anion . -
pubmed
From each block , serial semithin sections were cut , etched , and immunostained according to the peroxidase - antiperoxidase method with well - characterized polyclonal antisera that reacted specifically with
P0 glycoprotein and the
basic proteins
P1 and
P2 . -
pubmed
However , the presence of
UDP - D - glucuronic acid inhibited incorporation of
[ 14C ] xylose from
UDP - D -[ 14C ] xylose into beta-(1 - ---4)-xylan , and neither
S - adenosylmethionine nor
acetyl - CoA stimulated either the
xylosyltransferase or the
glucuronyltransferase . -
pubmed
The results indicate that the delayed
cardiotoxicity induced by
Dox is not mediated by an interference with the cardiac
beta - adrenoceptor function . -
pubmed
Thus , for these
ECPs , and multiple
antigen mixtures in general , the preferred immunization protocol should employ at least three hosts and utilize the cascade immunization of Thalhamer and Freund . -
pubmed
Differential effects of
forskolin on
LH and
GH release . -
pubmed
The effects of
forskolin , an agent which increases intracellular levels of
cAMP , on basal
luteinizing hormone (
LH ) and
growth hormone (
GH ) release and on
gonadotropin - releasing hormone (
GnRH)-stimulated
LH release were documented . -
pubmed
Secretory rates of both
LH and
GH increased in a concentration - dependent manner in response to a 1 - h challenge with 0.03 , 0.1 , 0.3 , 1 , or 3 microM
forskolin . -
pubmed
In response to 0.3 microM
forskolin , maximum
GH release was achieved within 15 - 20 min , after which secretion decreased . -
pubmed
In contrast ,
LH release increased gradually , became maximal at 1.5 - 2 h , and remained constant until the
forskolin was withdrawn . -
pubmed
Cells exposed to 10 nM
GnRH for 4 h exhibited a biphasic release of
LH with the interphase nadir occurring at 30 min . -
pubmed
The second phase of
LH release was enhanced by simultaneous addition of
forskolin with the
GnRH . -
pubmed
Whereas second phase release did not increase further , exposure of the cells to
forskolin for 60 or 120 min before
GnRH resulted in increased first - phase
LH release . -
pubmed
We suggest that , whereas our data are consistent with a role for
cAMP in mediating the acute release of
GH ,
cAMP may be involved in the process through which nonimmediately releasable
LH becomes available for release . -
pubmed
We have measured the length and determined the position of the
alkBAC operon on this fragment by
electron microscopy of R - loops . -
pubmed
The
enzyme preparation was applied to the affinity column in the presence of 10 mM
acetylcholine to increase the affinity of
CAT for
coenzyme A ; the
enzyme then was eluted with 10 mM
acetyl coenzyme A. -
pubmed
At the highest antibody concentration tested ,
anti - CAT 3 inhibited
acetylcholine synthesis 80 % . -
pubmed
Over a period of 20 months repeat cesarean sections were performed at Tripler Army Medical Center while a protocol with the following elements was used : ( 1 ) known last menstrual period ; ( 2 ) landmarks : positive urine
human chorionic gonadotropin test by 6 weeks , Doppler fetal heart tone by 12 weeks , date determination by examination before 10 weeks , fetoscope fetal heart tone by 20 weeks , and date determination by size before 30 weeks ; ( 3 ) date determination by midtrimester sonogram(s ) ; ( 4 ) normal third - trimester
glucose screening ; ( 5 ) biparietal diameter of 9.2 or 9.5 cm before scheduling . -
pubmed
Penicillin antibodies and
immunoglobulin production in newborn infants : studies with chemically modified bacteriophage . -
pubmed
In vitro effect of
reproterol upon pulmonary , cardiac , vascular and intestinal
3 ' , 5 '- monophosphate phosphodiesterase nucleoside . -
pubmed
The blood pressure ,
body weight , mean 24 hour urinary
electrolyte excretion , and
plasma renin activity in offspring whose parents both belonged to the top third of the distribution of blood pressure in their 5 - year age - group were compared with those in offspring whose parents belonged to the bottom third . -
pubmed
Cyclazocine decreased the levels of NA , and markedly increased the levels of
MHPG -
SP4 and
5 - HIAA in cortex , hypothalamus , midbrain and pons - medulla , while little change in
5 - HT concentration , except a decrease after the highest dose , was observed . -
pubmed
Extraction of unfractionated cells with 6 M
urea or solubilization in
Nonidet P-40 released
immunoreactive Gc protein , with physicochemical properties indistinguishable from those of
Gc purified from serum ( apparent MW 56 K ; pI 4.8 - 5.1 ) . -
pubmed
In addition , the theoretical expressions have been rearranged into forms suitable for determination of the relevant interaction parameters by simple graphical analysis , a procedure illustrated with results obtained in a partition equilibrium study of the
NADH - dependent elution of
rabbit muscle lactate dehydrogenase from Blue Sepharose . -
pubmed
The bone
nitrogen content remained relatively unchanged , but dry weights , DNA and
collagen contents were all decreased in plaque - treated bones . -
pubmed
subtilis strain Porton ,
PBP 2a had an altered mobility by
sodium dodecyl sulfate gel electrophoresis and was present in increased amounts . -
pubmed
Because the perfusion medium lacks
angiotensinogen ,
kininogen and
aldosterone , the natriuretic action in perfused kidneys may not be related to its inhibitory action on
angiotensin I converting enzyme or
kininase II . -
pubmed
Hemoglobin connecticut (
beta 21 ( B3 ) Asp leads to
Gly ): a
hemoglobin variant with low
oxygen affinity . -
pubmed
Structural analysis of the abnormal beta - chain indicated that the
amino acid substitution was at position 21 (
B3 ) , and involved the replacement of
aspartic acid with
glycine . -
pubmed
Various
GMCHA esters strongly inhibited the
proteinase activity and the order of the effects was closely correlated with that on the cell growth . -
pubmed
The
RANTES protein causes release of
histamine from basophils and is a chemoattractant for CD45RO /
CD4 + " memory " T lymphocytes , monocytes , and eosinophils . -
pubmed
CCK-8 - stimulated pancreatic protein output was inhibited by
PP in the dorsal motor nucleus in dose - dependent and site - specific manners . -
pubmed
The inhibitory effect of
PP on
CCK-8 - stimulated protein output was eliminated by vagotomy . -
pubmed
Influence of
thiols on the chlorinating effect of a
myeloperoxidase system . -
pubmed
Until recently
mucin tandem repeat protein cores were believed to exist in random -
coil conformations and to attain structure solely by the addition of
carbohydrates to
serine and
threonine residues . -
pubmed
Results of one- and two - dimensional
1H NMR correlation spectroscopy on these peptides confirm that the
muc-1 protein core is not a random -
coil secondary structure . -
pubmed
The primary immunodominant
mucin epitope , PDTRP , does not appear to attain a native conformation in the single repeat peptide ( 20
amino acids , starting with P ) , but is expressed on peptides with multiple repeats . -
pubmed
Pertussis toxin ( PT ) reduced the
oxytocin - stimulated formation of [
3H]IPs in a concentration - dependent manner . -
pubmed
However , a significant fraction of the
oxytocin response appears to be mediated by a PT - insensitive
G - protein , possibly a member of the
Gq family . -
pubmed
In further studies ,
endothelin-1 was co - infused with an inhibitor of
nitric oxide production ,
NG - monomethyl - L - arginine , or after oral administration of the irreversible inhibitor of
prostaglandin production ,
acetylsalicylic acid (
aspirin ) . -
pubmed
Although
glyceryl trinitrate partially prevented
endothelin-1 - induced venoconstriction ( maximum : 33 + /- 5 % ) , inhibition of
nitric oxide production did not affect
endothelin-1 - induced venoconstriction ( maximum : 55 + /- 4 % ) . -
pubmed
Prostacyclin was more effective at blocking the venoconstriction in response to
endothelin-1 than
glyceryl trinitrate ( maximum : 12 + /- 3 % ) , and there was substantial potentiation of
endothelin-1 - induced venoconstriction after pretreatment with
aspirin ( maximum : 90 + /- 3 % ) . -
pubmed
Inhibition of
45Ca release from calvariae treated with either 1 microM
hydrocortisone or 0.1 microM
dexamethasone was observed with 0.01 - 30 nM
salmon calcitonin ( sCT ) , 0.1 mM
acetazolamide , and 0.1 mM of the
bisphosphonate AHPrBP . -
pubmed
Differences between our sequence data on
CSAD and those reported previously were observed at two positions , which arose from a single
amino acid substitution and frame shift mutation . -
pubmed
Investigation of the signal transduction mechanisms induced by
RANTES in T cells revealed
tyrosine phosphorylation of multiple protein species with prominent bands at 70 - 85 and 120 - 130 kD. -
pubmed
Expression of
type II NOS by Hofbauer cells may indicate they are involved in surveillance against maternal immune insult or maternal pathogens whereby they secrete
nitric oxide to exert a cytostatic /
cytotoxic effect . -
pubmed
This putative receptor , designated
GFRalpha-3 , is closely related in
amino acid sequence and is nearly identical in the spacing of its
cysteine residues to both
GFRalpha-1 and
GFRalpha-2 . -
pubmed
Fibromodulin appeared as a single polydisperse band with an apparent molecular weight ( MW ) of 80,000 ( 80 kDa ) on
SDS - PAGE . -
pubmed
These data suggest that
fibromodulin is one of the small
PGs in the PDL - matrix and may fulfill construction and maintenance functions in this tissue . -
pubmed
In contrast , activation of Kir current by
somatostatin was unaffected , and inhibition of
calcium channels was only modestly attenuated . -
pubmed
The possibility that
PKC acted by phosphorylating
CB1 receptors was confirmed by demonstrating that
PKC phosphorylated a single
serine ( S317 ) of a fusion protein incorporating the third intracellular loop of
CB1 . -
pubmed
Mutating this
serine to
alanine did not affect the ability of
CB1 to modulate currents , but it eliminated disruption by
PMA , demonstrating that
PKC can disrupt ion channel modulation by receptor phosphorylation . -
pubmed
Synthesis and potent antifolate activity and
cytotoxicity of B - ring
deaza analogues of the nonpolyglutamatable
dihydrofolate reductase inhibitor
Nalpha -( 4 - amino-4 - deoxypteroyl )- Ndelta - hemiphthaloyl - L - ornithine (
PT523 ) . -
pubmed
Six new B - ring analogues of the nonpolyglutamatable antifolate
Nalpha -( 4 - amino-4 - deoxypteroyl )- Ndelta - hemiphthaloy l - L - ornithine (
PT523 , 3 ) were synthesized with a view to determining the effect of modifications at the 5- and/or 8 - position on
dihydrofolate reductase (
DHFR ) binding and
tumor cell growth inhibition . -
pubmed
The Ki for inhibition of
human DHFR by the
5 - deaza and
5 - methyl-5 - deaza analogues was about the same as that of 3 ( 0.35 pM ) , 11 - fold lower than that of
aminopterin (
AMT , 1 ) , and 15 - fold lower than that of
methotrexate (
MTX , 2 ) . -
pubmed
This trend was consistent with the published literature on the corresponding
DHFR inhibitors with a
glutamate side chain . -
pubmed
BACKGROUND :
Metallothionein (
MT ) , acting as an antioxidant and
zinc binding protein , may play an important role in regulation of apoptosis . -
pubmed
It is proposed that plasma
PLTP mass levels are related to
glucose metabolism rather than to lipid metabolism . -
pubmed
We have built up a pharmacophore model of the
ATP - binding site of the
epidermal growth factor receptor (
EGFR )
kinase and used it for the rational design of
kinase inhibitors . -
pubmed
Amongst these ,
4 - substituted - pyrrolo [ 2,3 - d ] pyrimidines , a new class of highly potent and selective inhibitors of the
EGFR kinase , have been identified and further optimized . -
pubmed
The most active derivatives inhibited the
EGFR tyrosine kinase with IC50 values between 1 and 5 nM. -
pubmed
In
EGF - dependent cellular systems ,
tyrosine phosphorylation , as well as c - fos mRNA expression , was inhibited with similar IC50 values . -
pubmed
Further successful application of this pharmacophore model led to the identification and optimization of
phenylamino - pyrazolo [ 4,3 - d ] pyrimidines and substituted
isoflavones and
quinolones , other classes of potent , selective , and
ATP competitive
EGFR kinase inhibitors with IC50 values in the low nanomolar range . -
pubmed
The aim of this study was to determine the frequency of the defective alleles in
CYP2D6 and
CYP2C19 in Africans and to test whether the genotype for
CYP2C19 is better correlated with the
proguanil /
cylcoguanil ratio than the
mephenytoin S / R ratio . -
pubmed
HFE binds to
transferrin receptor (
TfR ) , the receptor used by cells to obtain
iron in the form of diferric
transferrin (
Fe - Tf ) . -
pubmed
The results demonstrate that
HFE inhibits the
TfR :
Fe -
Tf interaction by binding at or near the
Fe - Tf binding site on
TfR , and that the
Fe - Tf :
TfR :
HFE ternary complex consists of one
Fe - Tf and one
HFE bound to a
TfR homodimer . -
pubmed
Estrogen replacement enhances acquisition of a spatial memory task and reduces deficits associated with hippocampal
muscarinic receptor inhibition . -
pubmed
Enhancement of
vitamin - K - dependent protein function by modification of the
gamma - carboxyglutamic acid domain : studies of
protein C and
factor VII . -
pubmed
No clinically significant interactions have been reported between
desloratadine and drugs that inhibit the
cytochrome P450 system , nor does the drug potentiate the adverse psychomotor effects of
alcohol . -
pubmed
in Group I anastomosis bursting pressure (
ABP ) was by 56 + /- 17 % higher at day 2 than in controls with no
pentoxifylline treatment . -
pubmed
In Group
II (
pentoxifylline : 2 mg/100 g , i.p . ) -
pubmed
In day care center (
DCC ) SY , the
penicillin resistant rate was 31.8 % , being much higher than the average level . -
pubmed
However in one
DCC , there could be a prevalence of some resistant clones which resulted in high
penicillin resistant rate . -
pubmed
A statistical analysis of published data using restricted maximum likelihood showed that the age of the bird and the dietary crude
protein concentration in test diets explained most of the variation in published
threonine requirements . -
pubmed
Hyaluronidase ,
adrenaline and normal saline were injected in the subcutaneous fat . -
pubmed
Results suggest that the requirement for
PTTH to stimulate
ecdysteroid production has been bypassed , i.e. -
pubmed
The apparent Km values for
NADP and
D - G3P or
DL - G3P were estimated to be 0.385 + /- 0.05 and 0.666 + /- 0.1 mM , respectively and the Vmax of the purified
protein was estimated to be 162.5 U mg(-1 ) . -
pubmed
pyogenes
GAPN was markedly inhibited by
sulfydryl - modifying reagent
iodoacetamide , these results suggest the participation of essential
sulfydryl groups in the catalytic activity . -
pubmed
Studies on
oxygenases ; enzymatic oxidation of
imidazoleacetic acid . -
pubmed
Sorbate hypersensitivity was detected in mutants of the
shikimate biosynthesis pathway , strains lacking the
PDR12 efflux pump or
WAR1 , a transcription factor mediating stress induction of
PDR12 . -
pubmed
Although induction of the majority of
sorbate - induced genes required
Msn2p / 4p , weak acid tolerance was unaffected by a lack of
Msn2p / 4p . -
pubmed
Ectopic expression of
PDR12 from the
GAL1 - 10 promoter fully restored
sorbate resistance in a
strain lacking
War1p , demonstrating that
PDR12 is the major target of
War1p under
sorbic acid stress . -
pubmed
A four - step purification protocol involving
ammonium sulfate precipitation of a 1.0 M
NaCl extract , ion - exchange chromatography on
DEAE -
Trisacryl M ,
lectin - affinity chromatography , and gel filtration chromatography resulted in electrophoretically pure
GhGLP1 . -
pubmed
While 1.0 M
NaCl extracts from 10 - 14
DPA fiber contained
superoxide dismutase and
phosphodiesterase activities ,
GhGLP1 could be separated from both enzyme activities by the purification protocol . -
pubmed
The identification and staging of these
tumors can be performed through the use of MRI and/or
CT scans and an
octreotide scintigraphy . -
pubmed
[ On the relevance of
protein kinase C to
lithium therapy in
bipolar disorder ] . -
pubmed
Valproate , which is dissimilar structurally to
lithium , shares most of the effects of
lithium at the level of
protein kinase C (
PKC ) . -
pubmed
Am80 is specific for the
retinoic - acid receptor - alpha / beta , and has therapeutic effects in
all - trans retinoic acid resistant
acute promyelocytic leukemia . -
pubmed
Am80 slightly inhibited the growth of both
myeloma cells and HUVECs , and remarkably inhibited the growth of HUVECs stimulated by
VEGF . -
pubmed
Am80 inhibited
VEGF - induced phosphorylation of
VEGF receptor . -
pubmed
Using CLSI methodology and disks containing
cefotetan alone and in combination with 400 mug of
boronic acid , 9 of 10 positive control
strains and 54 of 55
AmpC - PCR - positive clinical isolates were detected . -
pubmed
Boronic acid disks also enhanced detection of expanded - spectrum
beta - lactamases in
AmpC producers . -
pubmed
The level of
ribulose 1,5 - diphosphate was roughly equal in
chloroplasts showing immediate linear kinetics with respect to
CO ( 2 ) fixation and
chloroplasts which exhibited an initial lag . -
pubmed
The rapid loss of
ATP was ascribed to an
ATPase rather than to its utilization in
kinase reactions . -
pubmed
Data were obtained indicating that
triose phosphates similar to
pentose monophosphates and in contrast to
fructose 6 - phosphate ,
glucose 6 - phosphate and
glucose 1 - phosphate could be transported between
chloroplast and suspending medium . -
pubmed
In this review we will discuss pharmacoproteomic studies of drugs regulating
leukemia specific targets like
all - trans retinoic acid ,
histone deacetylase inhibitors , proteasome inhibitors and
tyrosine kinase inhibitors , as well as studies on drug resistance and graft - versus - host studies during stem cell transplantations . -
pubmed
Furthermore , HS induced oxidative burst , the early event of plant defense response to pathogen attack and other stress challenge ; the addition of putative inhibitors of
lipoxygenase , a key enzyme for
jasmonic acid biosynthesis , significantly inhibited HS - induced pacliatxel accumulation . -
pubmed
When skin is directly exposed to UVR , cutaneous intracellular catalytic
iron levels increase because of the release of
iron from
iron - binding proteins such as
ferritin . -
pubmed
In vivo
rat brain opioid receptor binding of
LY255582 assessed with a novel method using LC /
MS /
MS and the administration of three tracers simultaneously . -
pubmed
LY255582 is a pan
opioid selective receptor antagonist that has been shown to have high affinity for
mu , delta , and kappa receptors in vitro . -
pubmed
AIM : This study evaluated the influence of muscle mechanical afferent stimulation on the integrated arterial baroreflex control of the
sinus node during dynamic exercise . -
pubmed
CONCLUSION : These data suggest that the stimulation of mechanosensitive
stretch receptors is capable of modifying the integrated baroreflex control of
sinus node function by decreasing the cardiac vagal outflow during exercise . -
pubmed
After we began oral chemotherapy with
UFT - E 300 mg / day , the
CEA level normalized and a
CT scan revealed the liver
metastasis had disappeared within about 9 months . -
pubmed
The endogenous
tryptamine - based agonist
serotonin ( 5 - HT ; 1 ) and the
benzenesulfonyl - containing
tryptamine - derived
5 - HT6 receptor antagonist
MS-245 ( 4a ) were automatically docked with each of the 100 receptor models using a genetic algorithm approach . -
pubmed
Similar studies were conducted with the more selective
5 - HT6 receptor agonist
EMDT ( 5 ) and optical isomers of
EMDT - related analog 8 , as well as with optical isomers of
MS-245 ( 4a)-related and
benzenesulfonyl - containing
pyrrolidine 6 and
aminotetralin 7 . -
pubmed
The results indicate that the presence or absence of an
N1 - benzenesulfonyl group is a major determinant of the manner in which
tryptamine - related agents bind at
5 - HT6 serotonin receptors . -
pubmed
About 5 % of the egg cells and zygotes ( 2 - 3 from 50 ovules ) could be mechanically dissected within 2 h. When 0.1 %
cellulase and 0.1 %
pectinase were added to the
mannitol isolation solution , about 18 % of the egg cells ( 8 - 10 from 50 ovules ) could be isolated within 2 h. The egg cells isolated by mechanical dissection could be used for in vitro fertilization studies without any of the potentially deleterious effects of the enzymes on the plasma membrane of egg cell . -
pubmed
HTLV-1
tax - induced
NF - kappaB activation is synergistically enhanced by
12 - O - tetradecanoylphorbol-13 - acetate : mechanism and implications for
Tax oncogenicity . -
pubmed
In assessing the possibility of modulating
Tax pathogenic potential by external factors , we focused here on
12 - O - tetradecanoylphorbol-13 - acetate (
TPA ) which is a potent
protein kinase C (
PKC ) activator . -
pubmed
There are conflicting reports regarding the effect of
TPA and
PKC on
NF - kappaB. -
pubmed
We found that
TPA promoted
NF - kappaB nuclear translocation and the DNA binding of
p65 dimers through
PKC activation . -
pubmed
However , both
TPA and ectopically expressed
PKC had only a marginal effect on the transcriptional competence of these dimers , indicating that the DNA binding of such dimers is insufficient by itself for gene activation . -
pubmed
Notably , however , both
TPA and the ectopic
PKC displayed strong synergistic enhancement of the
Tax - induced activation of the
NF - kappaB transcriptional function . -
pubmed
In contrast ,
TPA and the ectopic
PKC only slightly elevated the low activation of the
NF - kappaB transcriptional capacity by
cytoplasmic Tax mutants , indicating that the nuclear translocation of
Tax was essential for this synergism . -
pubmed
Subsequent experiments suggested that
TPA contributed to this synergism by increasing the pool of free
p65 which
Tax could link to
CBP and elevate , thereby , the amount of a
p65 -
Tax -
CBP ternary complex that could bind to NF - kappaB - responsive promoters and stimulate their expression . -
pubmed
Leptin sensitivity of dissociated arcuate (
ARC ) and ventromedial ( VMN ) hypothalamic nucleus neurons from the 3- to 4 - wk - old offspring was assessed using fura-2
calcium imaging to monitor
leptin - induced changes in intracellular
calcium ( [
Ca ( 2 +)]( i ) ) as an index of neuronal activity . -
pubmed
Behavior and cellular evidence for
propofol - induced
hypnosis involving
brain glycine receptors . -
pubmed
BACKGROUND : It is well documented that several general anesthetics , including
propofol , potentiate
glycine receptor function . -
pubmed
Propofol - induced LORR was examined in the presence and absence of
strychnine ( a
glycine receptor antagonist ) ,
GABAzine ( a
gamma - aminobutyric acid A receptor antagonist ) , as well as
ketamine ( an antagonist of
N - methyl - D - aspartic acid subtype of
glutamate receptors ) . -
pubmed
These results suggest that neuronal
glycine receptors partially contribute to
propofol - induced
hypnosis . -
pubmed
AIM :
Alcohol in the media influences norms around use , particularly for young people . -
pubmed
Plasma pre beta - HDL formation is decreased by
atorvastatin treatment in
type 2 diabetes mellitus : Role of
phospholipid transfer protein . -
pubmed
UNLABELLED :
Atorvastatin lowers plasma
phospholipid transfer protein (
PLTP ) activity , which stimulates pre - beta - HDL generation in vitro . -
pubmed
We determined the effect of
atorvastatin on pre - beta - HDL formation and its relation with
PLTP activity in
type 2 diabetes . -
pubmed
RESULTS : Plasma pre - beta - HDL formation ,
triglycerides ,
LDL cholesterol ,
PLTP activity , and
CETP decreased after statin treatment ( all P<0.001 vs placebo ) , whereas
HDL cholesterol increased ( P<0.005 ) . -
pubmed
BACKGROUND AND PURPOSE : We compared the dose - dependent reductions in cellular
superoxide anion (
O ( 2 )(- ) ) by catalytic agents :
superoxide dismutase (
SOD ) ,
polyethylene glycol (
PEG)-SOD and the
nitroxide 4 - hydroxy-2,2,6,6 ,- tetramethylpiperidine-1 - oxyl (
tempol ) with uncharacterized antioxidants :
5,10,15,20 - tetrakis ( 4 - sulphonatophenyl ) porphyrinate iron ( III )( Fe - TTPS ) ,
( -)- cis-3,3 ' , 4 ' , 5,7 - pentahydroxyflavane ( 2R,3R )- 2 -( 3,4 - dihydroxyphenyl )- 3,4 - dihydro-1 ( 2H )- benzopyran-3,5,7 - triol (
- epicatechin ) ,
2 - phenyl-1,2 - benzisoselenazol-3 ( 2H )- one (
ebselen ) and
N - acetyl - L - cysteine (
NAC ) with the spin trap
nitroblue tetrazolium (
NBT ) and with the
vitamins or their analogues :
ascorbate ,
alpha - tocopherol and
6 - hydroxy-2,5,7,8 - tetramethylkroman-2 - carboxy acid (
trolox ) . -
pubmed
KEY RESULTS :
SOD ,
PEG - SOD ,
NAC and
tempol produced a similar maximum inhibition of
O ( 2 )(- ) of 80 - 90 % . -
pubmed
Effectiveness in disrupted and intact cells was similar for the permeable agents ,
PEG - SOD and
tempol , but was enhanced for
SOD . -
pubmed
Synthesis and evaluation of phosphopeptides containing
iminodiacetate groups as binding ligands of the
Src SH2 domain . -
pubmed
Phosphopeptide
pTyr - Glu - Glu - Ile ( pYEEI ) has been introduced as an optimal
Src SH2 domain ligand . -
pubmed
Peptides , Ac - K(IDA)pYEEIEK(IDA ) ( 1 ) , Ac - KpYEEIEK ( 2 ) , Ac - K(IDA)pYEEIEK ( 3 ) , and Ac - KpYEEIEK(IDA ) ( 4 ) , containing 0 - 2
iminodiacetate (
IDA ) groups at the N- and C - terminal
lysine residues were synthesized and evaluated as the
Src SH2 domain binding ligands . -
pubmed
Fluorescence polarization assays showed that peptide 1 had a higher binding affinity ( K(d ) = 0.6 microM ) to the
Src SH2 domain when compared with
Ac - pYEEI ( K(d ) = 1.7 microM ) , an optimal
Src SH2 domain ligand , and peptides 2 - 4 ( K(d ) = 2.9 - 52.7 microM ) . -
pubmed
The binding affinity of peptide 1 to the
SH2 domain was reduced by more than 2 - fold ( K(d ) = 1.6 microM ) upon addition of
Ni ( 2 + ) ( 300 microM ) , possibly due to modest structural effect of
Ni ( 2 + ) on the
protein as shown by circular dichroism experimental results . -
pubmed
The degree of
fibrosis in lung was evaluated by the content of
hydroxyproline (
chloramine T method ) and area of
collagen ( Masson stain ) in lung . -
pubmed
Impact of
Y143 HIV-1 integrase mutations on resistance to
raltegravir in vitro and in vivo . -
pubmed
Despite this drug 's activity against viruses resistant to other antiretrovirals , failures of
raltegravir therapy were observed , in association with the emergence of resistance due to mutations in the
integrase coding region . -
pubmed
We also describe histology and
glucocorticoid receptor (
GR ) protein expression in response to inhaled
NO treatment [ 40 ppm
NO gas or
NO donors
sodium nitroprusside (
SNP ) or
diethylamine NONOate (
DEA /
NO ) ] . -
pubmed
SNP and
GC provided protection against bronchoconstriction to a similar degree in the model of severe
asthma . -
pubmed
Using
1 - H -[ 1,2,4 ]- oxadiazolo -[ 4,3 - a ]- quinoxalin-1 - one (
ODQ ) , a soluble
guanylate cyclase inhibitor , we found this effect of
NO donors to be mediated through a
cGMP - independent mechanism . -
pubmed
In the severe model , prolonged
NO treatment restored or even increased the nuclear levels of
GR . -
pubmed
PF-00337210 is a potent , selective small molecule inhibitor of
VEGFRs and has been under consideration for the treatment of age - related
macular degeneration . -
pubmed
Stress can lead to changes in the serum level of many hormones including glucocorticoids ,
catecholamines , growth hormone and
prolactin . -
pubmed
The effectiveness of
UV254 reduction ( 52 - 81 % ) and colour removal ( 51 - 88 % ) was determined by the effectiveness of filtration in removing
post - coagulation turbidity , as well as by the type of applied polymer . -
pubmed
Estrogen - induced recruitment of
TIP60 requires direct binding of
TIP60 to
ERalpha and the action of chromatin - remodeling ATPase
BRG1 , leading to increased recruitment of histone methyltransferase
MLL1 and increased monomethylation of
histone H3 at
Lys4 . -
pubmed
Thus , complex cooperation of
TIP60 with
ERalpha and other chromatin - remodeling enzymes is required for
estrogen - induced transcription . -
pubmed
We conducted laboratory culture studies to determine the effects of three pesticides {
carbaryl ,
glyphosate , and
thiophanate - methyl [
TM ;
Topsin - M(R ) ( Cerexagri - Nisso LLC ) ] } on Bd zoospore production and zoosporangia growth . -
pubmed
All pesticides significantly inhibited zoospore production ; however ,
glyphosate and
TM were more effective at doing so than
carbaryl . -
pubmed
Our data suggest that
carbaryl and
glyphosate are equally effective at inhibiting both zoosporangia and zoospore production ; however ,
TM is selectively toxic to zoospores but not zoosporangia . -
pubmed
The objectives of the present study were : ( i ) to compare the immunohistochemical expression of
estrogen ,
progesterone and
prolactin receptors and
HER-2 / neu in
breast cancer before and after neoadjuvant chemotherapy ; and ( ii ) to correlate the expression of these
tumor markers with partial
tumor response to neoadjuvant chemotherapy . -
pubmed
Additional indications are
hypoxia ( S(p)O(2 ) & lt ; 90 % despite
oxygen insufflation and after exclusion of
tension pneumothorax ) , severe
traumatic brain injury [ Glasgow Coma Scale (
GCS ) & lt ; 9 ] ,
trauma - associated hemodynamic instability [
systolic blood pressure (
SBP ) & lt ; 90 mmHg ] and severe
chest trauma with
respiratory insufficiency ( respiratory rate & gt ; 29 / min ) . -
pubmed
We have previously hypothesized that
mental fatigue is caused partly by neuronal
brain damage through the activation of
N - methyl - D - aspartate ( NMDA ) receptors by
quinolinic acid (
QUIN ) , a metabolite of
tryptophan (
TRP ) . -
pubmed
Memantine , an
NMDA receptor antagonist , inhibited not only the reduction in swimming times but also the
neuronal damage induced by water - immersion . -
pubmed
These results suggest that neuronal
brain damage by an endogenous
NMDA receptor agonist other than
QUIN participates in combined
fatigue by water immersion . -
pubmed
This enzyme , like most lipolytic enzymes from family VIII , possesses two possible nucleophilic
serines located in an
S - X - X - K beta - lactamase motif and a
G - X - S - X - G lipase motif . -
pubmed
The
serine in the S - X - X - K motif is a catalytic residue , but the role of
serine within the common
lipase consensus sequence G - X - S - X - G has not yet been systematically studied . -
pubmed
Here , the previously reported time - intensive procedure for purification of recombinant
LipBL was replaced by one - step metal - affinity chromatography purification in the presence of
ATP . -
pubmed
Site - directed mutagenesis performed to map the active site of
LipBL revealed that mutation of
serine and
lysine in the
beta - lactamase motif ( S(72)-M - T - K(75 ) ) to
alanine abolished the enzyme activity of
LipBL , in contrast to mutation of the
serine in the
lipase consensus motif ( S321A ) . -
pubmed
Furthermore , mutagenesis was performed to understand the role of the G - X - S - X - G motif and other
amino acids that are conserved among
family VIII esterases . -
pubmed
SLC26A9 - mediated
chloride secretion prevents mucus obstruction in
airway inflammation . -
pubmed
BA levels were determined using proton signals ( delta(H ) 7.53 and 7.98 ) referenced to
2 - dimethyl-2 - silapentane-5 - sulfonate - d ( 6 ) sodium salt (
DSS - d(6 ) ) after simple solvent extraction from processed foods . -
pubmed
pneumoniae lacks
catalase , the question of how it controls
H ( 2 ) O ( 2 ) levels is of critical importance . -
pubmed
This study shows that
tpxD encodes a functional
thiol peroxidase involved in the adjustment of
H ( 2 ) O ( 2 ) homeostasis in the cell . -
pubmed
Kinetic experiments showed that recombinant
TpxD removed
H ( 2 ) O ( 2 ) efficiently . -
pubmed
Mass spectrometry analysis demonstrated that
TpxD Cys(58 ) undergoes selective oxidation in vivo , under conditions where
H ( 2 ) O ( 2 ) is formed , confirming the
thiol peroxidase activity . -
pubmed
The challenge with D39 and TIGR4 with
H ( 2 ) O ( 2 ) resulted in
tpxD upregulation , while
psaBCA expression was oppositely affected . -
pubmed
However , the challenge of
DeltatpxD mutants with
H ( 2 ) O ( 2 ) did not affect
psaBCA , implying that
TpxD is involved in the regulation of the
psa operon , in addition to its scavenging activity . -
pubmed
NO can exert its biological function through different ways , such as the modulation of gene expression , the mobilization of second messengers , or interplays with
protein kinases . -
pubmed
The obtained nanostructured
carbon /
TiO ( 2 ) nanocomposites are characterized by X - ray diffraction ( XRD ) , high - resolution transmission electron microscopy ( HRTEM ) , UV - vis diffuse reflectance spectra (
DRS ) , Raman spectra , X - ray photoelectron spectroscopy ( XPS ) and photoluminescence spectra (
PL ) , and then are used as catalysts for photocatalytic
hydrogen production . -
pubmed
YM155 , a novel molecular targeted agent , suppresses
survivin , which is overexpressed in many
tumor types . -
pubmed
Annexin V /
propidium iodide staining followed by flow cytometric analysis was used to detect apoptosis in cell culture . -
pubmed
Hypertension ,
diabetes , smoking , total
cholesterol ,
triglycerides ,
LDLc ,
HDLc and TT genotype of the
eNOS gene were independent risk factors for the development of
PCAD . -
pubmed
However , multiple genes are likely to contribute , either acting independently or epistatically , with the
CYP2C9 -
VKORC1 -
warfarin test panel , an example of a clinically used gene - gene - dug interaction . -
pubmed
I discuss here further instances of gene - gene - drug interactions , including a proposed dynamic effect on statin therapy by genetic variants in both a transporter (
SLCO1B1 ) and a metabolizing enzyme (
CYP3A4 ) in liver cells , the main target site where statins block
cholesterol synthesis . -
pubmed
ovata to induce oxidative stress in mussels exposed under laboratory conditions excepting for a certain increase of
catalase ,
glutathione reductase and
glutathione peroxidases activities , and a significantly higher capability to neutralize
peroxyl radicals in mussels exposed for 14 days . -
pubmed
In cell models ,
FTO overexpression reduced
ghrelin mRNA
N6 - methyladenosine methylation , concomitantly increasing ghrelin mRNA and peptide levels . -
pubmed
Efficacy exposure - response relationships of the
CCR5 antagonist
maraviroc were evaluated across two phase III clinical trials . -
pubmed
Prognostic factors for treatment success ( in decreasing order of Akaike information criterion (
AIC ) change ) were :
maraviroc treatment , high - weighted overall susceptibility to background treatment , absence of an undetectable
maraviroc concentration , high baseline
CD4 count ( BCD4 ) , low viral load ( VL ) , race ( other than black ) , absence of non - R5 baseline tropism ( BTRP ) , and absence of
fosamprenavir (
FPV ) . -
pubmed
The
maraviroc doses studied ( 300 or 150 mg with potent
CYP3A4 inhibitors once ( q.d. -
pubmed
Histone deacetylases (
HDACs ) are involved in the removal of
acetyl groups from intracellular proteins . -
pubmed
Namely ,
HOP2 - mediated strand exchange does not require ATP and , in contrast to
DMC1 , joint
molecules formed by
HOP2 are more sensitive to mismatches and are efficiently dissociated by
RAD54 . -
pubmed
Dietary
lycopene , angiogenesis , and
prostate cancer : a prospective study in the prostate - specific antigen
era . -
pubmed
Because angiogenesis is a strong progression factor , an endpoint of lethal
prostate cancer may be more relevant than an endpoint of indolent
prostate cancer for
lycopene in the
era of highly prevalent prostate - specific antigen screening . -
pubmed
The synthesized
silver nanoparticles were characterized by UV - Vis , Infrared spectroscopy ( IR ) , Transmission Electron Microscopy (
TEM ) , X - ray Diffraction studies ( XRD ) , Nanoparticle Tracking Analysis ( NTA ) , Energy Dispersive Spectroscopy ( EDS ) , and Cyclic Voltammetry ( CV ) . -
pubmed
The
ethanol content was measured by a fully validated headspace -
GC - FID method . -
pubmed
Structural manipulation on the
catecholic fragment of
dopamine D ( 1 ) receptor agonist
1 - phenyl - N - methyl - benzazepines . -
pubmed
The
8 - hydroxyl group , other than the
7 - hydroxyl was confirmed crucial to the interaction with the
dopamine D1 receptor . -
pubmed
7 -( m - Chlorophenyl ) methylamino - and
7 -( m - or o - tolyl ) methylamino - substituted benzazepines 13b - d displayed Ki values of 270 - 370 nM at the
D1 receptor , which were slightly more potent than that of parent compound 1 . -
pubmed
In addition ,
7 -( arylmethyl ) amino - benzazepines 13a - c were found possessing high binding affinities less than 10 nM at the
5 - HT2A receptor . -
pubmed
Among them , the non - substituted
7 - benzylamino analogue 13a was the most potent showing a Ki values of 4.5 nM at the
5 - HT2A receptor and a 5 - HT2A /
D1 selectivity of 147 . -
pubmed
AIM OF THE STUDY : The present study was designed to investigate the neuroprotective effects of
rotigotine , a
D3 / D2 / D1 dopamine receptor agonist , against the two powerful complex I inhibitors ,
1 - methyl-4 - phenylpyridinium (
MPP + ) and
rotenone , in primary mesencephalic cell culture relevant to
PD . -
pubmed
RESULTS :
Rotigotine alone did not influence the survival of
tyrosine hydroxylase immunoreactive ( THir ) neurons except at 10 microM , it significantly decreased the number of THir neurons by 40 % compared to untreated controls . -
pubmed
It is mediated primarily by a developmentally regulated expression of the
NKCC1 and
KCC2 chloride importer and exporter respectively . -
pubmed
The GABAergic depolarization acts in synergy with
N - methyl - d - aspartate ( NMDA ) receptor - mediated and voltage - gated
calcium currents to enhance intracellular
calcium exerting trophic effects on neuritic growth , migration and synapse formation . -
pubmed
The neuroprotective effect of
lovastatin on
MPP(+)-induced
neurotoxicity is not mediated by
PON2 . -
pubmed
Lovastatin produced no change in
paraoxonase-2 (
PON2 ) activity . -
pubmed
However , studies have reported inconsistent findings regarding the association between NTs and
fatty acid (
FA ) composition . -
pubmed
,
vanillin , alkaloids ) metabolisms ,
COMT genes might not be neutral markers , and represent candidate functional markers for resistance , aromatic or medicinal properties of Vanilla species . -
pubmed
Metabolic syndrome was defined as & gt;=3 of the following : elevated fasting
glucose , high
triglycerides , low
high - density - lipoprotein cholesterol , elevated systolic /
diastolic blood pressure , and increased waist circumference . -
pubmed
Our results indicate that : 1 ) endotoxin tolerance can be induced in astrocytes and results in
TNFalpha and
Cox2 mRNA induction decrease upon secondary stimulation ; 2 ) tolerance is revealed on the level of
TNFalpha release and
Cox2 protein expression ; 3 )
PPAR agonists
GW7647 ,
L-165041 , and
rosiglitazone control
Cox2 mRNA expression levels under conditions of endotoxin tolerance . -
pubmed
In particular ,
rosiglitazone ( a
PPARgamma agonist ) induces
Cox2 mRNA expression , while
GW7647 ( a
PPARalpha agonist ) and
L-165041 ( a
PPARbeta agonist ) suppress the expression . -
pubmed
Aquaporin 5 (
AQP5 ) is an
androgen - regulated member of a family of small hydrophobic integral transmembrane water channel proteins regulating cellular water homeostasis and growth signaling . -
pubmed
Furthermore , treatment with an anti - MM agent ,
lenalidomide , caused ubiquitination and proteasomal degradation of
Blimp-1 , leading to the de - repression of a new
Blimp-1 direct target ,
CULLIN 4A (
CUL4A ) , and reduced
Aiolos levels . -
pubmed
Accordingly ,
lenalidomide - induced cell
death was partially rescued by reintroduction of
Blimp-1 or knockdown of
CUL4A. -
pubmed
We also showed that interruption of
Blimp-1 /
Aiolos regulatory pathways contributes to
lenalidomide - mediated anti - MM activity . -
pubmed
Sacubitril /
valsartan ( formerly known as
LCZ696 ) is a first - in - class
angiotensin receptor neprilysin inhibitor ( ARNI ) that simultaneously suppresses
RAAS activation through blockade of
angiotensin II type 1 receptors and enhances vasoactive peptides including NPs through inhibition of
neprilysin , the enzyme responsible for their degradation . -
pubmed
Urine Concentrating Capacity ,
Vasopressin and
Copeptin in
ADPKD and
IgA Nephropathy Patients with
Renal Impairment . -
pubmed
Copeptin and
AVP increased significantly in a similar way in both groups . -
pubmed
Using in vitro and in vivo SUMOylation assays , we found that
JMJD2A is SUMOylated on
lysine 471 by
KSHV K - bZIP , a viral
SUMO-2/3 - specific
E3 ligase , in a SUMO - interacting motif ( SIM)-dependent manner . -
pubmed
Bradykinin is responsible for
angioedema attacks and is produced from contact phase activation secondary to failed
C1Inh control . -
pubmed
Both
phenylephrine (
PE ) and
norepinephrine ( NE ) are commonly used for induced
hypertension , but the impact of the initial choice of vasopressor on the efficacy , adverse effects , or outcome after hemodynamic therapy for
DCI is unknown . -
pubmed
Furthermore , we observed an enhanced accumulation of compatible solute (
proline ) and increased levels of
abscisic acid (
ABA ) and antioxidants in
miR156OE genotypes . -
pubmed
Consequently , we report an increased accumulation of
carbon - centered radicals in cells lacking
TDP1 , using electron spin resonance spectroscopy . -
pubmed
Overexpression of the antioxidant enzyme
superoxide dismutase 1 (
SOD1 ) reduces
carbon - centered adducts and protects
TDP1 - deficient cells from oxidative stress . -
pubmed
Together , our data show that
TDP1 resolves mtPDBs , thereby regulating mitochondrial gene transcription and
oxygen consumption by oxidative phosphorylation , thus conferring cellular protection against reactive
oxygen species - induced damage . -
pubmed
Genes involved in mitochondrial transport ,
calcium uptake , mitophagy , or fission and fusion were largely dispensable for axon regrowth , with the exception of
eat-3 /
Opa1 . -
pubmed
Surprisingly , many genes encoding components of the electron transport chain were dispensable for regrowth , except for the
iron - sulfur proteins
gas-1 ,
nduf-2.2 ,
nduf-7 , and
isp-1 , and the putative
oxidoreductase rad-8 . -
pubmed
Moreover , loss of function in
isp-1 reduced the enhanced regeneration caused by either a gain - of - function mutation in the
calcium channel
EGL-19 or overexpression of the
MAP kinase DLK-1 . -
pubmed
While the cellular function of
RAD-8 remains unclear , our genetic analyses place
rad-8 in the same pathway as other
electron transport genes in axon regeneration . -
pubmed
Unexpectedly ,
rad-8 regrowth defects were suppressed by altered function in the
ubiquinone biosynthesis gene
clk-1 . -
pubmed
However , serotype 42 has only O - acetylation at
3 - beta - galactofuranose , an observation consistent with its loss of
WciG functionality , which is associated with O - acetylation at the 2 - position and subsequent reaction with typing antiserum 35a . -
pubmed
The result showed that the epitope recognized by
5D3 is located within 173 - 240
amino acid of the
ALV - p27 protein . -
pubmed
As a result , this study aimed to determine whether : ( a ) differences exist in re - offence rates of convicted drink - drivers between : the period between committing the drink - driving offence and licence disqualification ( pre - licence disqualification ) , during the period of licence disqualification , and after being re - licensed (
post - licence restoration ) ; and ( b ) differential effects of offence rates are evident based on Blood
Alcohol Content ( BAC ) , gender , age , repeat offender status and crash involvement at the time of offence . -
pubmed
The pathway for its biosynthesis , initiating with
UDP - GlcNAc , requires three enzymes referred to as
PglF ,
PglE , and
PlgD. -
pubmed
Specifically ,
PglF catalyzes the first step in the pathway , namely , the
dehydration of
UDP - GlcNAc to
UDP-2 - acetamido-2,6 - dideoxy - alpha - d - xylo - hexos-4 - ulose . -
pubmed
Most members of the
SDR superfamily contain a characteristic signature sequence of
YXXXK where the conserved
tyrosine functions as a catalytic acid or a base . -
pubmed
Strikingly , in
PglF , this residue is a
methionine . -
pubmed
On the basis of the data reported herein , a new catalytic mechanism for a
SDR superfamily member is proposed that does not require the typically conserved
tyrosine residue . -
pubmed
A positive correlation between the serum
uric acid concentration of serum
insulin ( r = 0.27 , p & lt;0.001 ) , age ( r = 0.21 , p & lt;0.002 ) and
BMI patients ( r = 0.26 , p & lt;0.002 ) has been shown . -
pubmed
Synthesis and Molecular Modeling Studies of
N '- Hydroxyindazolecarboximidamides as Novel
Indoleamine 2,3 - Dioxygenase 1 (
IDO1 ) Inhibitors . -
pubmed
To discover novel
IDO1 inhibitors , we designed and synthesized a series of
N '- hydroxyindazolecarboximidamides . -
pubmed
Among the compounds synthesized , compound 8a inhibited both
tryptophan depletion and
kynurenine production through the
IDO1 enzyme . -
pubmed
Molecular docking studies revealed that 8a binds to
IDO1 with the same binding mode as the analog ,
epacadostat (
INCB24360 ) . -
pubmed
Here , we report the synthesis and biological evaluation of these
hydroxyindazolecarboximidamides and present the molecular docking study of 8a with
IDO1 . -
pubmed
MicroRNA-200a activates
Nrf2 signaling to protect osteoblasts from
dexamethasone . -
pubmed
miR-200a expression activated
Nrf2 signaling , which inhibited
dexamethasone - induced reactive
oxygen species production and OB-6 cell
death / apoptosis . -
pubmed
Thereafter , membranes were treated with / without
TNFalpha ( 50 ng / mL ) and/or
progesterone for 48 hours , harvested , and homogenized . -
pubmed
RESULTS :
TNFalpha increased apoptosis ( measured by
caspase-3 , - 8 , and - 9 activities ) in fetal membranes , and this effect was abrogated by
progesterone . -
pubmed
Under basal conditions ,
progesterone suppressed expression of the proapoptotic protein ,
BID , by 0.45 ( 0.14)-fold , and increased expression of the antiapoptotic proteins ,
Bcl-2 and
XIAP ; no change was seen in
BAX or
FLIP . -
pubmed
In contrast ,
TNFalpha increased
BID expression by 5.15 ( 2.92)-fold , which was prevented by pretreatment with
progesterone . -
pubmed
CONCLUSIONS :
Progesterone inhibits apoptosis in fetal membranes by suppressing expression of the proapoptotic protein ,
BID ( for both basal and
TNFalpha - induced apoptosis ) , and upregulating expression of the antiapoptotic proteins ,
XIAP and
Bcl-2 ( under basal conditions only ) . -
pubmed
Role of
RACK1 on cell proliferation , adhesion , and
bortezomib - induced apoptosis in
multiple myeloma . -
pubmed
The present study aimed to explore the function of
RACK1 on the cell proliferation , adhesion , and
bortezomib - induced apoptosis in
MM . -
pubmed
We also found that altered expression of
RACK1 is associated with
bortezomib - mediated MM cell apoptosis . -
pubmed
Oestrogens control postnatal mammary gland development acting on a subset of mammary epithelial cells ( MECs ) , termed sensor cells , which are
ERalpha - positive by immunohistochemistry ( IHC ) and secrete paracrine factors , which stimulate
ERalpha - negative responder cells . -
pubmed
To identify
SH - BC-893 - induced cell signaling events that disrupt lysosomal trafficking , we compared phosphorylation profiles in cells treated with SH - BC-893 or
C2 - ceramide , a non - vacuolating
sphingolipid that does not impair lysosomal fusion . -
pubmed
These analyses combined with functional assays uncovered the differential regulation of
Akt and
Gsk3b by SH - BC-893 ( vacuolating ) and
C2 - ceramide ( non - vacuolating ) . -
pubmed
Photomodulation of exogenous GFP and endogenous mitotic
kinesin-5 (
Eg5 ) gene expression in cells was achieved using the prepared caged
Dex- p - siRNA nanoparticles . -
pubmed
Seasonal variation of
arsenic and
antimony in surface waters of small subarctic lakes impacted by legacy mining pollution near Yellowknife ,
NT , Canada . -
pubmed
Moreover ,
WGLSF effectively reduced the hepatic levels of free
fatty acids and the pro - inflammatory
cytokines TNF - alpha ,
IL-6 , and
NF - kappaB. -
pubmed
Intracellular
ATP Delivery Causes Rapid Tissue Regeneration via Upregulation of
Cytokines ,
Chemokines , and Stem Cells . -
pubmed
Conversely , the
wounds treated with
ATP - vesicles showed significantly higher expression of
MCP-1 and
stem cell markers (
CD44 ,
CD106 ,
CD146 , and
CD34 ) at day 1 , significantly higher
IL-1beta and
TNF - alpha expression from day 1 - 4 , and significantly higher
VEGF - A ,
VEGF - D , and
VEGFR-2 expression from day 4 - 6 when compared to the controls . -
pubmed
Structural modifications of compound 2 , an
angiokinase inhibitor reported by our group were performed , which led to the discovery of
methyl ( Z )- 3 -((( 4 -( 2 - methyl-5 -(( 4 - methylpiperazin-1 - yl ) methyl )- 1H - pyrrol-1 - yl ) phenyl ) amino )( phenyl ) methylene )- 2 - oxoindoline-6 - carboxylate ( 7h ) . -
pubmed
Based upon the results of spectroscopic analyses and
ECD measurements , the structures of new compounds were determined to be the 6 - 6'- ( 1 and 2 ) and
1 - 5 '- ( 3 ) C - C connected pseudo - dimeric anthraquinones , respectively . -
pubmed
We created a
critical illness severity scoring system ( CISSS ) using age , comorbidities , heart rate , mean arterial blood pressure , temperature , respiratory rate , hematocrit , white blood cell count ,
creatinine ,
sodium ,
glucose ,
albumin ,
bilirubin ,
bicarbonate , use of invasive mechanical ventilation , and whether the admission was surgical or not . -
pubmed
The engineered
FN /
PNIPAM DAE system significantly increased the symmetric renewal of ADMSCs , particularly between passages 7 and 9 (
p7 -
p9 ) , before it dropped down to the level of the control ( FN - coated TC
polystyrene ) . -
pubmed
FN supported also the osteogenic response of ADMSCs ( apart from the previous observations with plain
PNIPAM substrata ) indicated by the significant increase of
alkaline phosphatase (
ALP ) activity at days 7 and 14 . -
pubmed
The femoral artery contractile changes induced by the extracellular
nucleotides and
dinucleotide were similar when
nitric oxide synthase was inhibited . -
pubmed
RIPK1 is involved in mediating the interaction between
AMPK and
TSC2 and facilitate
TSC2 phosphorylation at
Ser1387 . -
pubmed
RIPK1 - deficient cells are unable to cope with energetic stress and are vulnerable to low
glucose levels and
metformin . -
pubmed
The
post - mineralogy analysis of mineral dust proxies added to ASW showed a diatom - induced increased formation of
goethite , where the amount of
goethite formed correlated with observed enhanced growth . -
pubmed
,
triolein ,
elastin , keratin ,
fibrillar collagen , and
type IV collagen ) associated with primary
NPC and
post - treatment recurrent
NPC tissue compared to
normal nasopharyngeal tissue . -
pubmed
To understand
AMZ action ,
glutathione content ,
superoxide dismutase enzyme activity , and lipid peroxidation were evaluated in CC . -
pubmed
Moreover ,
cytidine deaminase expression was significantly increased in the CTC lines obtained after failure of the first- and second - line
5 - fluorouracile - based treatments , suggesting that these CTCs can eliminate this specific drug and resist to therapy . -
pubmed
Abdominal computed tomography (
CT ) was examined after completion of 8 cycles of adjuvant chemotherapy with
capecitabine combination
oxaliplatin . -
pubmed
Effects of adaptation ,
prolactin ,
cortisol and
actinomycin D on plasma osmotic balance and (
Na +- K +) ATPase in gill and kidney . -
pubmed
Albumin ,
R4 ,
R2 , and
R1 all bound to an affinity column for
albumin ( Cibacron blue 3G - A dye coupled to
agarose ) and were eluted in that order by a
KSCN gradient . -
pubmed
It was not possible to incorporate labeled
amino acid into
albumin or the
albumin - like
R proteins with pituitary incubates , indicating that
albumin - like proteins were not synthesized de novo by pituitary glands . -
pubmed
The function of these endogenous
albumin fragments is unknown , but
albumin fragments produced in vitro by other investigators are known to potentiate
bradykinin , a
hypotensive peptide . -
pubmed
Conformational analysis of
ApA ,
ApC ,
CpA ,
CpC ,
GpG ] . -
pubmed
The
quinazoline couterpart of
folic acid (
5,8 - deazafolic acid ) as well as its
10 - methyl analogue has been shown to be an effective inhibitor of
thymidylate synthetase from several different sources . -
pubmed
Mixtard , Penmix and Actrapid were the best understood
insulins , Insulatard , Humulin Zn and
Protaphane the worst . -
pubmed
Low flow - rates and small particle diameters maximise the displacements , as well as improve the resolution between the parent
insulin and its
desamido contaminant . -
pubmed
The gradient slope did not substantially affect the separation between the parent
insulins , but , due to the relative " S " values of the solutes , an increase in gradient slope improved the separation of the parent
insulin from its
desamido compound . -
pubmed
The values of catalytic characteristics of the modified form in tRNA(
Tyr ) aminoacylation reaction ( Km = 1.19 microM and kcat = 2.99 min-1 ) are close to those obtained for the main form of the
enzyme ( 0.69 microM and 2.97 min-1 , respectively ) . -
pubmed
Amino acid composition of the low - molecular form of
tyrosyl - tRNA - synthetase has been determined . -
pubmed
A proteolytically modified form of
tyrosyl - tRNA - synthetase possesses , like the main form , the affinity to high - molecular rRNA but it is eluted from the column filled with rRNA - sepharose at lower
salt concentration ( 50 mM
KCl ) as compared to the main form of the
enzyme ( 100 mM
KCl ) . -
pubmed
1H resonance assignment and secondary structure determination of the dimerization domain of
transcription factor LFB1 . -
pubmed
It is shown that partial ( 85 - 96 % ) inhibition with any one of three inhibitors is effective when the inhibitor is added in
G1 or in S , but more complete ( 99 % ) inhibition is effective only if the inhibitor is added after cells have entered S. In addition , cells begin to respond to the inhibition after a lag that increases as DNA synthesis in the early part of S is progressively inhibited with
aphidicolin , indicating that competence to respond is achieved only after cells have reached a particular point in the replication of their genome . -
pubmed
Within the first 20 days after
MMI withdrawal the increase of serum T3 levels was correlated with the observed decrease of
serum TSH concentrations . -
pubmed
Six months after
MMI withdrawal basal
serum TSH concentration was normal while an exaggerated response to
TRH was observed . -
pubmed
Monovalent cations (
Na + ,
Li + , and
Cs + ) , divalent cations (
Mg2 + ,
Ca2 + , and
Mn2 + ) , and
guanine nucleotides did not significantly affect the specific binding of
[ 3H ] ICI-198615 to
guinea pig lung LTD4 receptors . -
pubmed
MeSH : D 12.776.543.750.830.630 Receptors ,
estrogen G 6.184.154.803.817.349 Receptors ,
estrogen G 12.803.817.349 Receptors ,
estrogen D 12.776.543.750.830.700 Receptors ,
progesterone G 6.184.154.803.817.777 Receptors ,
progesterone G 12.803.817.777 Receptors ,
progesterone A 5.360.319.114.170 Broad ligament A 5.360.319.114.803 Round ligament -
pubmed
The 5'-terminal
triphosphate , present on the primary transcript , remains intact through 3'-terminal maturation and through subsequent transport of the
tRNA to the cytoplasm . -
pubmed
A reagent and a derivatization procedure have been developed which result in trimesylation of
all - OH and -
NH2 groups on
proteins :
serine ,
threonine ,
tyrosine , and
lysine side chains are all completely derivatized . -
pubmed
Conditions for solubilization of
proteins in
anhydrous organic solvents for the derivatization are described ; removal of blocking groups results in the polar , highly water - soluble protein derivative which behaves as a monomer during gel permeation chromatography in simple aqueous buffers even in the absence of detergents . -
pubmed
The octapeptide
Thr - Pro - Pro - Thr - Pro - Ser - Pro - Ser , which occurs between residues 233 and 240 , has an IC50 value of 0.26 mM for the
type 1 proteinase and 0.50 mM for the type 2 enzyme . -
pubmed
Acetylation of the
octapeptide N - terminal
amino group lowers affinity for the
type 1 proteinase sixfold but does not change binding to the type 2 enzyme . -
pubmed
Amidation of the C - terminal
carboxyl group does not change binding to the
type 1 proteinase but improves IC50 for the type 2 enzyme . -
pubmed
Simultaneous blockade of both the N- and C - termini drastically lowers affinity of the
octapeptide for both
proteinases . -
pubmed
Sequential replacement of the
hydroxy amino acids in the blocked
octapeptide with
cysteine yields a series of inhibitors that generally bind to the neisserial
IgA1 proteinases as well as or better than the unblocked octapeptide . -
pubmed
The most effective inhibitor contains a
cysteine residue at position 6 (
P3 ' ) and has an IC50 value for the
type 2 IgA1 proteinase of 50 microM. -
pubmed
The N terminus of the
SulA protein [
amino acid ( aa ) residues 1 - 30 ] has diverged extensively during the evolution of Enterobacteriaceae , whereas the central region ( aa residues 31 - 149 ) has been well conserved . -
pubmed
Serum
proteins are precipitated by the addition of 30 microliters of 40 %
trichloroacetic acid solution . -
pubmed
Competition experiments indicate a substrate specificity of specific
3H - Ro 5 - 4864 binding similar to the properties already demonstrated for the specific binding of this ligand to peripheral
benzodiazepine binding sites in many other tissues . -
pubmed
The
carbohydrate moieties exposed on enterobacteria before and after antibody binding have been tested with fluorescent
lectins . -
pubmed
typhimurium
MS and R 10 enhanced the affinity for
RCA - I ( Gal ) and to a smaller extent for
WGA (
GlcNAc ) which may be explained by exposure of
IgG oligosaccharide . -
pubmed
The corresponding
sugars N - acetylglucosamine ,
mannose and
glucose are present in the
carbohydrate chain of the secretory component as well as in
IgA indicating that when
SIgA antibody binds its
sugar components are exposed . -
pubmed
The assay used a
alpha - naphthyl acetate as substrate and measured the combined activity of the enzymes
acetylcholinesterase (
AChE ,
E.C. - pubmed
The effect of increased hydrophobicity at position 6 of
luteinizing hormone - releasing hormone (
LH - RH ) has been investigated by the incorporation of a series of 15 very hydrophobic , unnatural
D - amino acids at this position . -
pubmed
Parallel assessment by the Scatchard method suggests , as expected , that
albumin is the principle protein moiety responsible for binding
piretanide . -
pubmed
The
antibody titer measured for the
5 - O - acetyl-6 - O - stearoyl compound 9 was comparable to that obtained with
6 - O - stearoyl - MDP at 50 micrograms , and both compounds were more active than
MDP at 5 micrograms . -
pubmed
Other molecules , including
angiotensin II ,
bradykinin ,
thrombin , and
catecholamines , are beginning to appear to play major roles in control of smooth - muscle replication in vivo as well . -
pubmed
In agreement with previous reports , our deletion analysis indicates that a region encompassing
amino acids 411 to 419 ( KRLLSEKKT ) in the
cytoplasmic domain of
CD4 was required to confer
Vpu sensitivity . -
pubmed
However , six specific substitution mutations within this region did not confer
CD4 resistance to
Vpu , suggesting that neither the
amino acid sequence nor the charge of the
amino acids in this region was critical to
Vpu - induced
CD4 degradation . -
pubmed
A
dileucine motif that is important for internalization of
CD4 and
Nef - induced
CD4 down - regulation was also not required for
Vpu - induced
CD4 degradation . -
pubmed
These events coincide with the ability of
GM - CSF to increase the intracellular levels of both
cyclic 3 ' , 5 '- adenosine monophosphate (
cAMP ) and
cyclic 3 ' , 5 '- guanosine monophosphate (
cGMP ) in T cells , to increase the binding of
( gamma-35S ) GTP to T - cell membranes , and to enhance
GTPase activity as determined by increased hydrolysis of
32P -
GTP . -
pubmed
IL-2 also induced
IL-2R expression ,
cyclic nucleotide secretion , and
G - protein activation . -
pubmed
Furthermore ,
GTP binding and
GTPase activity induced by
GM - CSF or
IL-2 were inhibited by the addition of
cholera toxin (
CT ) , but not
pertussis toxin ( PT ) . -
pubmed
Coumermycin A1 is an inhibitor of
DNA gyrase , an enzyme that catalyzes supercoiling of DNA and is required for bacterial DNA replication . -
pubmed
burgdorferi was more susceptible than many other eubacteria to
coumermycin as well as
novobiocin , another
coumarin antibiotic ; this contrasted with its relative resistance to the
DNA gyrase inhibitors
nalidixic acid ,
oxolinic acid , and
ciprofloxacin . -
pubmed
In contrast ,
WEB 2086 , a
platelet - activating factor antagonist , had no effect on lung
albumin leak . -
pubmed
Inducible ,
Ca ( 2 +)- independent nitric oxide ( NO ) synthase activity in the heart is elevated during
endotoxemia and the resulting excess release of
NO depresses cardiac contractile function . -
pubmed
High affinity
3H - dopamine ( DA ) and
14C GABA uptakes as well as immunocytochemical staining of
tyrosine hydroxylase (
TH)-containing cells were used as specific functional markers of DA and
GABA neuron viability . -
pubmed
The presence of increased apoptosis was verified by ultrastructural and
terminal transferase - mediated
dUTP nick end labeling analyses . -
pubmed
By 3 DIV ,
synapsin - positive puncta ( previously shown to correspond to presynaptic specializations ) were detected surrounding the
soma and proximal dendritic processes , whereas comparable aggregations of
synelfin did not appear until several days later . -
pubmed
Genome segment 10 of CfCPV was found to be 1171 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 258 residues ( Mr of 29,795 ) , consistent with an apparent Mr of 30.5 kDa determined by
SDS - PAGE of purified
polyhedrin . -
pubmed
Analysis of HIV-1
reverse transcriptase gene mutations in infected children treated with
zidovudine . -
pubmed
After 15 min of UV action form of the humoral activity products of
POL was increased ; the redox system of
vitamin E was mobilized ; the membranes
proteins was opened ; the neutral lipid fraction in membranes was decreased , and its polarity was increased . -
pubmed
Removal of all potential cleavage sites between the
extracellular epidermal growth factor domain and the transmembrane domain by substitution and small deletions ( & lt;11
amino acid residues out of 21 ) did not significantly reduce
ARIA release , whereas larger deletions abolished it . -
pubmed
We propose that cleavage occurs independently of
amino acid sequence at a short distance from the
epidermal growth factor domain , unless sterically hindered by the nearby secondary structure . -
pubmed
Fasting
glucose , serum lipids and activated partial
thromboplastin time ,
factor VIII fibrinogen ,
factor VII ,
antithrombin III ,
protein C , and
von Willebrand factor measurements were made . -
pubmed
Norepinephrine modulates single hypothalamic arcuate neurons via
alpha ( 1 ) and beta adrenergic receptors . -
pubmed
The results showed that : ( 1 ) of 236 neurons selected randomly and tested with NE application , 137 ( 58.0 % ) were excited , 67 ( 28.4 % ) were inhibited , and 32 ( 13.6 % ) failed to respond ; ( 2 ) substitution of low
Ca ( 2 +)-high
Mg ( 2 + ) artificial cerebrospinal fluid ( ACSF ) for normal ACSF abolished the NE - induced inhibitory effect but failed to abolish the excitatory effect ; ( 3 ) both the NE - induced excitatory and inhibitory effects were antagonized partly by
phentolamine ,
prazosin , and
propranolol but not by
yohimbine ; ( 4 )
naloxone and
glibenclamide , a blocker of
adenosine triphosphate - sensitive ( K ( ATP ) ) channels , blocked the NE - induced inhibitory effect ; and ( 5 ) neurons that were inhibited by NE were also inhibited by
morphine and
cromakalim , an agonist of K(
ATP ) channels , and moreover , the
morphine - induced inhibitory effect could be blocked by
glibenclamide , while the
cromakalim - induced inhibitory effect was not blocked by
naloxone . -
pubmed
These results imply that : ( a ) NE excites arcuate neurons through a mechanism that is insensitive to lowering the extracellular
Ca ( 2 + ) suggesting a direct postsynaptic response through
alpha ( 1 )- and beta - adrenergic receptors , while NE inhibits cells through at least an inhibitory interneuron in arcuate and so is dependent on a
Ca ( 2 +)-sensitive presynaptic release mechanism ; and ( b ) the inhibitory interneuron may be opioidergic , being excited first through
alpha ( 1 )- and beta - adrenergic receptors , after which the released opioids inhibit the neurons being recorded with an involvement of activation of K(
ATP ) channels . -
pubmed
Response of periodontal ligament fibroblasts and gingival fibroblasts to pulsating fluid flow :
nitric oxide and
prostaglandin E2 release and expression of tissue non - specific
alkaline phosphatase activity . -
pubmed
Periodontal ligament fibroblasts , but not gingival fibroblasts , responded to fluid flow with significantly elevated release of
nitric oxide and decreased expression of tissue non - specific
alkaline phosphatase activity . -
pubmed
Even partial suppression of
prostacyclin ( without coincident inhibition of platelet
COX-1 - dependent
thromboxane formation ) by
COX-2 inhibitors may be undesirable in
acute vascular occlusive syndromes . -
pubmed
The recombinant
Fab fragment was eluted as a single peak in a
sodium chloride gradient . -
pubmed
The purified
Fab57P fragment showed one band in
SDS - PAGE . -
pubmed
The transfection procedure has been optimized for large ( 225 - cm(2 ) ) culture flasks and
F - ATM can be purified to near homogeneity as judged by
SDS - PAGE . -
pubmed
Human liver disease decreases
methacrylyl - CoA hydratase and
beta - hydroxyisobutyryl - CoA hydrolase activities in
valine catabolism . -
pubmed
BACKGROUND :
Methacrylyl - coenzyme A ( MC - CoA ) hydratase and
beta - hydroxyisobutyryl - coenzyme A ( HIB - CoA ) hydrolase are key enzymes regulating the toxic concentration of
MC - CoA generated in
valine catabolism . -
pubmed
Similarly , cell impermeant
albumin - conjugated
progesterone , but not
albumin - conjugated
17 beta - estradiol , decreased 5 - HT+U stimulated VMC
Ca ( 2 + ) responses ( 250 + /- 34 % of basal 30 min after stimulation ) back to the prestimulation level ( 113 + /- 17 % of basal ) in 25 min ( half time = 7 min ) . -
pubmed
Taken together , these experiments suggest that
NPS3121 has an active mechanism to suppress the accumulation of defense transcripts and
phytoalexin biosynthesis in bean . -
pubmed
To investigate a role of
PTEN , a
tumor suppressor gene , in the regulation of
hyaluronic acid ( HA)-induced invasion of
glioma cells , we examined the secretion of
MMP-9 in various
glioma cells with or without a functional
PTEN gene . -
pubmed
The aim of our present study was to formulate , by the w/o / w emulsification process ,
LMWH microparticles as potential oral carriers prepared with biodegradable (
poly - epsilon - caprolactone and
poly - lactic - co - glycolic acid ) and nonbiodegradable polycationic polymers (
Eudragit RS and RL ) , used alone or blended . -
pubmed
For each formulation ,
LMWH released from microparticles preserved its biological activity as shown by the
antifactor Xa activity . -
pubmed
To achieve this goal , an anti -
RyR antibody was newly raised against a synthetic peptide corresponding to an
amino acid sequence that was conserved among all sequenced
RyRs . -
pubmed
Electron microscopic images of the carp myocardium showed that the
SR was observed largely as the subsarcolemmal cisternae and the reticular
SR , suggesting that the
RyR is localized in the junctional and corbular SR . -
pubmed
CONCLUSION : The technical methods in this study may help detect the immunogenicity and immunoprotection of
OmpL1 and develop more safe , highly effective rBCG bearing leptospiral
antigen with long - lasting protection . -
pubmed
Hemoglobin , blood cell morphology , serum protein - bound
iodine , serum proteins , serum lipids and serum total
cholesterol estimations , urinalyses , and agglutination studies for
brucellosis were carried out . -
pubmed
every 28 days for three cycles ; 2 ) anti -
CD20 chimeric monoclonal antibody (
rituximab ) 375 mg / m2 i.v . -
pubmed
RESULTS : In WKY , repeated administration of
ivabradine produced reduction of
MBP ,
DBP and HR , without change of
systolic blood pressure (
SBP ) . -
pubmed
Midazolam metabolism was also inhibited in vitro by typical chemical inhibitors of
CYP3A , such as
ketoconazole ,
erythromycin and
diltiazem , and the apparent K(i ) values for
ketoconazole ,
erythromycin and
diltiazem were 0.127 , 94.2 and 29.6 microM , respectively . -
pubmed
CYP3A inhibitors increased plasma
midazolam concentrations when
midazolam and
CYP3A inhibitors were co - administered orally . -
pubmed
However , the pharmacokinetic parameters of
midazolam were not changed by treatment with
CYP3A inhibitors when
midazolam was given intravenously . -
pubmed
The drug - drug interaction responsible for
CYP3A enzyme(s ) inhibition was observed when
midazolam and inhibitors were co - administrated orally . -
pubmed
Analysis of
sulfur and
nitrogen odorants using solid - phase microextraction and
GC -
MS . -
pubmed
OBJECTIVE : The only approved pharmacological approach for the symptomatic treatment of
Alzheimer ' s disease in Japan is the use of a
cholinesterase inhibitor ,
donepezil hydrochloride . -
pubmed
Upon analysis of covariance , where those confounding variables ( age , sex , disease duration , education , MRI interval ,
APOE genotype , and baseline
Alzheimer ' s Disease Assessment Scale score ) were entered into the model as covariates , the effect of
donepezil treatment on
hippocampal atrophy remained significant . -
pubmed
In the first parts , advances in three major methods , grafting (
post - synthetic modification ) , co - condensation ( direct synthesis ) , and techniques related with periodic mesoporous
organosilicates , are explained . -
pubmed
Heme binding by the N - terminal fragment 1 - 44 of
human growth hormone . -
pubmed
The binding property is specific for the N - terminal helix of
hGH , since
heme binding does not occur with fragment 45 - 191 or the entire
protein . -
pubmed
These residues can act as specific ligands for the
heme -
iron , as observed with
cytochromes . -
pubmed
The
CD4 /
CD8 ratio was positively correlated with the
Gln level ( r=0.704 , P<0.05 ) , while the monocyte
HLA - DR expression lowered as compared to control . -
pubmed
Pyruvate dehydrogenase kinase 4 (
PDK4 ) is a key regulatory enzyme involved in switching the energy source from
glucose to
fatty acids in response to physiological conditions . -
pubmed
The
enzyme may be important in
glycine and
serine synthesis . -
pubmed
A comparison between the enzymic activity of
isocitrate lyase and the amount of
glycine and
serine synthesized during logarithmic growth indicated that the activity was great enough to account for all of the
carbon entering these
amino acids during that stage of growth . -
pubmed
METHODS :
Arthritis was induced by immunisation with
human type II collagen in complete Freund 's
adjuvant and measured by footpad swelling , clinical score and histology . -
pubmed
Omega-3 polyunsaturated fatty acids affect lipopolysaccharide - induced maturation of dendritic cells through
mitogen - activated protein kinases p38 . -
pubmed
OBJECTIVE : The
omega-3 polyunsaturated fatty acids (
PUFAs ) play a key role as immune response modulators and suppressors of immunologic functions , such as lymphocyte proliferation ,
cytokine production , and cell surface molecular expression in T lymphocytes , monocytes , and natural killer cells . -
pubmed
RESULTS : The
omega-3 PUFAs reduced expression levels of
costimulatory molecules CD80 and
CD86 and
major histocompatibility complex HLA - DR . -
pubmed
IL-12 and
tumor necrosis factor - alpha levels decreased significantly in the
EPA and
DHA groups . -
pubmed
The
omega-3 PUFAs significantly inhibited LPS - induced
p38 phosphorylation . -
pubmed
CONCLUSION : The
omega-3 PUFAs may inhibit LPS - induced DC maturation and upregulate
cytokine production . -
pubmed
BACKGROUND / AIMS : The
polyunsaturated fatty acid arachidonic acid ( AA ) has been implicated in beta - cell defence mechanisms and
prostaglandin (
PG ) products of
cyclooxygenase ( COX ) 2 action confer resistance to
alloxan - induced apoptosis in
insulin - secreting RIN cells . -
pubmed
CONCLUSION : These data show that AA and its
COX2 - generated metabolite ,
PGE ( 2 ) , can protect beta - cells from apoptosis . -
pubmed
Respiratory plasticity following intermittent
hypoxia : roles of
protein phosphatases and reactive
oxygen species . -
pubmed
Here , we review accumulating evidence suggesting that the pattern - sensitivity of pLTF arises substantially from differential reactive
oxygen species formation and subsequent
protein phosphatase inhibition during intermittent compared with sustained
hypoxia in or near phrenic motor neurons . -
pubmed
Nitric oxide (
NO ) , a short - lived gaseous free radical , synthesized from
L - arginine by
NO synthases (
NOS ) , is a potent mediator of biologic responses involved in the pathogenesis of
autoimmune rheumatic diseases , such as
systemic lupus erythematosus (
SLE ) and
rheumatoid arthritis (
RA ) . -
pubmed
Most biological necessary
NO is produced by the family of three
NOS . -
pubmed
We constructed a single - chain variable fragment miniantibody (
G11 -
scFv ) directed toward the transactivation domain of
c - Myc , which is fused with the internalization domain Int of
Antennapedia at its
carboxyl terminus ( a cargo - carrier construct ) . -
pubmed
RESULTS : Although the
methacholine threshold was related to all parameters that represent airway caliber and lability , Min%Max PEF had the strongest correlation with
AHR . -
pubmed
RESULTS : Levels of
CEA protein and transcript were found to be higher in
FUL - treated cells than in controls . -
pubmed
However , when target cells were exposed to
OXA before but not after
FUL treatment , the up - regulation of
CEA was partially inhibited . -
pubmed
CONCLUSION : These results suggest that target cells must be exposed to
OXA after but not before treatment with the
fluoropyrimidine in order to exploit drug - induced up - regulation of
CEA . -
pubmed
This finding appears to provide useful information to design chemo - immunotherapy protocols based on
FUL +
OXA , combined with host 's immunity against
CEA directed
cancer vaccines . -
pubmed
Aromatase inhibitors have proven to have disease - free survival advantage when used after 5 years of
tamoxifen with a delay of up to 2.8 years . -
pubmed
SUMMARY : In summary , at least further 4 years of
aromatase inhibitor treatment after the first 5 years of adjuvant
tamoxifen is recommended in postmenopausal endocrine responsive early
breast cancer , nowadays . -
pubmed
Treatment of various types of cells with the
mitochondrial ATP - sensitive K (+ ) channel opener ,
diazoxide , preconditions cells to subsequent
injuries and inhibits apoptosis . -
pubmed
Here we demonstrate that
diazoxide inhibited the proliferation of
lung cancer cells as well as the transcription of cell cycle - related protein
Cyclin D1 . -
pubmed
Cyclin D1 overexpression inhibited the negative role of
diazoxide in cell cycle progression . -
pubmed
We further explored the mechanisms by which
diazoxide affected
Cyclin D1 transcription and found that the
beta - catenin transcription factor was downregulated by
diazoxide , with a corresponding decrease in
Cyclin D1 promoter activity . -
pubmed
O - GlcNAc modification of radial glial
vimentin filaments in the developing chick brain . -
pubmed
We examined the
post - translational modification of intracellular proteins by
beta - O - linked N - acetylglucosamine (
O - GlcNAc ) with regard to neurofilament phosphorylation in the developing chick optic tectum . -
pubmed
Labeling of brain sections together with Western blot analyses showed
O - GlcNAc modification of a few discrete proteins throughout development , and suggested
vimentin as the protein in radial glia . -
pubmed
These results indicate a regulated pattern of
O - GlcNAc modification of
vimentin filaments , which in turn suggests a role for
O - GlcNAc - modified intermediate filaments in radial glia , but not in neurons during brain development . -
pubmed
BACKGROUND : An association of the Tyr402His variant of the
complement factor H (
CFH ) gene with age - related
macular degeneration (
AMD ) has been shown in several Caucasian populations , while studies for an association with other single
nucleotide polymorphisms ( SNP ) of complement system genes have produced inconsistent results . -
pubmed
Biochemical and mechanical analysis revealed a significant increase of cell mass ,
4 - hydroxyproline ,
collagen , and strength in the hybrid heart valve leaflets compared to controls . -
pubmed
RESULTS : Adipose tissue recovery during the dynamic phase of catch - up fat is accompanied by increased adipocyte number with smaller diameter , increased expression of genes for adipogenesis and de novo lipogenesis , increased
fatty acid synthase activity , increased proportion of
saturated fatty acids in
triglyceride ( storage ) fraction but not in phospholipid ( membrane ) fraction , and no impairment in
insulin signaling . -
pubmed
CONCLUSIONS : These findings suggest that increased adipose tissue
insulin stimulation and consequential increase in intracellular
glucose flux play an important role in initiating catch - up fat . -
pubmed
Once activated , the machinery for lipogenesis and adipogenesis contribute to sustain an increased
insulin - stimulated
glucose flux toward fat storage . -
pubmed
Rational combination therapy combines medications with complementary mechanisms of action , such as a
calcium channel blocker ( CCB ) and a
renin -
angiotensin -
aldosterone system (
RAAS ) inhibitor ; it is often more efficacious than monotherapy and allows the use of lower doses of the individual components , which usually results in improved tolerability . -
pubmed
The results of histopathological examination of specimens from
CB1 receptor and
anandamide injected group indicate that I / R
injury has regressed . -
pubmed
CONCLUSION : The protective effect of endogenous
anandamide on I / R injury may take place through
CB2 receptors in the small intestine ;
NO and
prostaglandin , which are activated through the stimulation of
CB2 receptors may be responsible for this protective effect ( Fig . -
pubmed
After exclusion of the milk
proteins ,
SMX and
TMP were transferred to the analytical column ( an
octyl column ) and the separation of the compounds from one another and from other endogenous milk components was achieved . -
pubmed
This case illustrates that
TBc can develop in multiple organs during
adalimumab therapy , and thus , the awareness of serious complications of multiple organs and atypical extrapulmonary pattern of
TBc during anti -
TNF - alpha therapy needs to be increased . -
pubmed
For the primary study , we tested the effects of decreasing cholinergic neurotransmission by systemic administration of the
muscarinic antagonist
atropine . -
pubmed
The effect of
ellagic acid on platelet activation as measured by the quantification of
P - selectin using flow cytometry . -
pubmed
We assessed the similarities of
ellagic acid to known
COX-2 specific and nonspecific
COX inhibitors by examining their effects on platelet activation via use of
P - selectin flow cytometry . -
pubmed
Our findings suggest that
ellagic acid is likely a nonspecific
COX inhibitor . -
pubmed
It also suggests that combining
ellagic acid with a known nonspecific
COX inhibitor such as
ketorolac may cause a significant decrease in platelet activity and an increase in
blood loss . -
pubmed
The
Plasmodium falciparum Ca ( 2 +)- ATPase PfATP6 : insensitive to
artemisinin , but a potential drug target . -
pubmed
Since
artemisinin is a powerful anti - malarial drug which has been proposed to target a particular
Ca2 + - ATPase (
PfATP6 ) in the parasite , it has been important to characterize the molecular properties of this enzyme . -
pubmed
PfATP6 conserves all sequences and motifs that are important for the function and/or structure of a
SERCA , such as two high - affinity
Ca2 + -binding sites , a
nucleotide - binding site and a phosphorylation site . -
pubmed
Our data fully confirm that
PfATP6 is a
SERCA , but with a distinct pharmacological profile : compared with
SERCA1a , it has a lower affinity for
thapsigargin and much higher affinity for
cyclopiazonic acid . -
pubmed
Hydrogen - bonding capability of a templating
difluorotoluene nucleotide residue in an
RB69 DNA polymerase ternary complex . -
pubmed
Here , we report crystal structure of an
RB69 DNA polymerase L561A / S565G / Y567A triple mutant ternary complex with a templating dF opposite
dTTP at 1.8 A - resolution . -
pubmed
In this structure , direct
hydrogen bonds were observed between : ( i ) dF and the incoming
dTTP , ( ii ) dF and residue G568 of the
polymerase , and ( iii ) dF and ordered water molecules surrounding the nascent base pair . -
pubmed
Effect of
tetracyclines on
IgE allergic responses and
asthma . -
pubmed
We found that
tetracyclines and chemically modified
tetracyclines (
CMT ) that lack antibiotic activity prevent
IgE production , making them ideal candidates for anti -
allergy therapy . -
pubmed
Tetracyclines significantly suppressed expression of phosphorylated
p38 MAP kinase by
CD4 + and
CD8 + T cells but not B cells or monocytes in vivo and in vitro . -
pubmed
Enzyme closure and
nucleotide binding structurally lock
guanylate kinase . -
pubmed
The
GK enzyme is subject to large conformational changes , leading from an open to a closed form , which are further influenced by the presence of
nucleotides . -
pubmed
Our data emphasizes the role of
magnesium , highlights a
water channel permitting active site hydration , and reveals a structural lock that stabilizes the closed form of the enzyme . -
pubmed
Deciphering
hERG channels : molecular basis of the rapid component of the delayed rectifier
potassium current . -
pubmed
hERG1a channels are tetramers consisting of four 1159
amino acid long proteins and have electrophysiological properties similar , but not identical , to native I(
Kr ) . -
pubmed
Alternative transcription of
hERG1 leads to a protein with a 56
amino acid shorter N - terminus , known as
hERG1b . -
pubmed
Changes in tissue
oxygen concentrations have been shown to produce changes in relaxation rate
R1 of water . -
pubmed
The aim of the study was to improve the sensitivity of
oxygen enhanced R1 imaging by exploiting the higher solubility of
oxygen in lipids ( as compared with water ) to sensitively monitor changes in tissue
oxygen levels by selectively measuring the
R1 of lipids . -
pubmed
report the upregulation of
IL-6 and
IL-8 after the activation of the unfolded protein response ( UPR ) following exposure of melanocytes to
phenols . -
pubmed
Binding of
IGFBP7 and
IGF-1 to
IGF1R was mutually exclusive , and the N - terminal 97
amino acids of
IGFBP7 were important for binding to the extracellular portion of
IGF1R and for preventing its activation . -
pubmed
( 18 ) F - FECH - PET /
CT has been proved to be an imaging agent for
prostate carcinoma . -
pubmed
From 18 confirmed bone involvement cases ,
( 18 ) F - FECH - PET /
CT identified correctly the bone involvement in 15 cases with overall sensitivity of 83.3 % . -
pubmed
The lesion - related results show that the sensitivity of each investigation differs with the anatomical regions , and by comparing both results ,
( 18 ) F - FECH - PET /
CT was mostly superior to bone scan ; however , without a statistical significance ( P & gt ; 0.1 ) . -
pubmed
In conclusion , no significant gain in sensitivity was achieved using bone scan compared with
( 18 ) F - FECH - PET /
CT . -
pubmed
We evaluated the carriage of
chlorhexidine resistance genes and
chlorhexidine susceptibility in
MRSA before and after introduction of an institutional
MRSA control programme incorporating
chlorhexidine - based decolonization in 2004 . -
pubmed
Carriage of qacA and smr and having a
chlorhexidine MIC & gt;=2 mg / L did not increase overall amongst
MRSA BSI isolates ; however , qacA carriage increased in CC22 compared with in CC30 ( OR , 7.21 ; 95 % CI , 1.32 - 39.17 ) . -
pubmed
CONCLUSIONS : A successful
infection control programme was associated with the selection of qacA linked with a higher
chlorhexidine MIC in one dominant endemic
MRSA clone ( CC22 ) , but not another ( CC30 ) . -
pubmed
Ligand binding - induced stabilization of the
Gal-7 dimer was supported by several lines of evidence : MD - based calculations of interaction energies between ligand - loaded and ligand - free states , gel filtration data and hetero - FRET spectroscopy that indicate a highly reduced tendency for dimer dissociation in the presence of
lactose , CD - based thermal denaturation showing that the transition temperature of the
lectin is significantly increased in the presence of
lactose , and saturation transfer difference (
STD ) NMR using a molecular probe of the monomer state whose presence is diminished in the presence of
lactose . -
pubmed
EPR studies also allow direct identification of the monoreduced
PTCDI species and spectroscopic measurements confirm the delocalization of electronic density around the
carbonyl moieties of the reduced species . -
pubmed
STAT pathway in the regulation of
zoledronic acid - induced apoptosis in
chronic myeloid leukemia cells . -
pubmed
The COCs were morphologically evaluated and randomly allocated to be cultured in either a control maturation medium or supplemented with 400 ng / mL
eGH , for 30 h at 38.5 C in air with 5 %
CO2 . -
pubmed
Compared to control , COCs incubated with
eGH had : more oocytes that reached metaphase
II ( 35/72 , 48.6 % vs. 60/89 , 67.4 % , respectively ; P=0.02 ) ; greater concentrations of
testosterone ( 0.21 + - 0.04 vs. 0.06 + - 0.01 ng / mL ; P=0.01 ) ,
progesterone ( 0.05 + - 0.01 vs. 0.02 + - 0.00 ng / mL ; P=0.04 ) , and
oestradiol ( 76.80 + - 14.26 vs. 39.58 + - 8.87 pg / mL ; P=0.05 ) in the culture medium , but no significant differences in concentration of
androstenedione . -
pubmed
A hospital based cross - sectional survey including , demographic profile , history of
CKD -
MBD symptoms , measurement of serum
calcium ,
phosphate ,
parathyroid hormone ,
25 hydroxy vitamin D (
25 ( OH ) D ) and
alkaline phosphatase ; lateral abdominal X - rays for
abdominal aortic calcification ( AAC ) and echocardiography for
valvular calcification (
VC ) was carried out . -
pubmed
Indeed , genetic ablation of
Trp53 , or
pharmacological inhibition of its protein product
p53 , rescues significantly the
cardiovascular defects of
Tbx1 heterozygous and hypomorphic mutants . -
pubmed
Hexameric
ATP - dependent
proteases and protein remodeling machines use conserved loops that line the axial pore to apply force to substrates during the mechanical processes of protein unfolding and translocation . -
pubmed
Here we demonstrate that
DNAX - activating protein of 12 kDa (
DAP12 ) , a transmembrane adaptor protein that contains an immunoreceptor
tyrosine - based activation motif , is a critical regulator of microglial function after
nerve injury . -
pubmed
The effects of structural characteristics on the
chlorpyrifos microcapsule particle size , entrapment rate (
ER ) , pesticide loading (
PL ) , and release behaviors in
ethyl alcohol were investigated . -
pubmed
The
ER and
PL varied with the BA / St monomer ratio ,
chlorpyrifos / monomer core - to - shell ratio , and
EGDMA cross - linker content with consequence that suitable
PL was estimated to be smaller than 3.09 % and the highest
ER was observed as 96.74 % . -
pubmed
Influences of
polyethylene glycol molar mass ( M
PEG ) and tie line lengths (
TLL ) on purification factor ( PF ) , yields in top ( Y T ) and bottom ( Y B ) phases and partition coefficient ( K ) were evaluated . -
pubmed
First scheme resulted in 65.9 %
enzyme yield and PF of 2.16 in salt - enriched phase with clarified homogenate ( M
PEG 1500 g mol(-1 ) ,
TLL 40 % ) ; Y B of 75.2 % and PF B of 2.9 with unclarified homogenate ( M
PEG 1000 g mol(-1 ) ,
TLL 35 % ) . -
pubmed
The highest PF value of integrated process was 2.26 in bottom phase ( M
PEG 1500 g mol(-1 ) ,
TLL 40 % ) . -
pubmed
Maximum response achieved was PFB = 3.3 ( M
PEG 1500 g mol(-1 ) ,
TLL 40 % ) . -
pubmed
Enzyme characterization showed
G6P Michaelis - Menten constant ( K M ) equal 0.07 - 0.05 ,
NADP (+ ) K M 0.02 - 1.98 and optimum temperature 70 C , before and after recovery . -
pubmed
Anti -
fibrillation propensity of a
flavonoid baicalein against the fibrils of
hen egg white lysozyme : potential therapeutics for
lysozyme amyloidosis . -
pubmed
In vivo
lysozyme fibrillation was simulated under in vitro conditions using a strong denaturant
GdHCl at 3 M concentration . -
pubmed
Sharp decline in the ANS fluorescence intensity compared to the partially unfolded states , almost 20 - fold increase in
ThT fluorescence intensity , increase in absorbance at 450 nm suggesting turbidity , negative ellipticity peak in the far - UVCD at 217 nm , red shift of 50 nm compared to the native state in Congo red assay and appearance of a network of long rope - like fibrils in transmission electron microscope (
TEM ) analysis suggested HEWL
fibrillation . -
pubmed
Anti -
fibrillation potency of
baicalein against the preformed fibrils of
HEWL was investigated following
ThT assay in which there was a dose - dependent decrease in
ThT fluorescence intensity compared to the fibrillar state of
HEWL with the maximum effect observed at 150 - muM
baicalein concentration , loss of negative ellipticity peak in the far - UVCD region , dip in the Rayleigh scattering intensity and absorbance at 350 and 450 nm , respectively , together with a reduction in the density of fibrillar structure in
TEM imaging . -
pubmed
The injection of
helium did not change the
T2 spectra of the coals . -
pubmed
In contrast , the
T2 spectra peaks of micro - capillary water gradually decreased and those of macro - capillary and bulk water increased with time after the injection of
CO2 . -
pubmed
We report herein
perovskite solar cells using solution - processed
silver nanowires ( AgNWs ) as transparent top electrode with markedly enhanced device performance , as well as stability by evaporating an ultrathin transparent
Au (
UTA ) layer beneath the spin - coated AgNWs forming a composite transparent metallic electrode . -
pubmed
Disturbances of folate metabolism due to
genetic abnormalities or the presence of autoantibodies to
folate receptor alpha (
FRalpha ) can impair physiologic processes dependent on
folate , resulting in a variety of
developmental disorders including
cerebral folate deficiency syndrome and
autism spectrum disorders . -
pubmed
Pyrazolylamine Derivatives Reveal the Conformational Switching between Type I and Type
II Binding Modes of
Anaplastic Lymphoma Kinase (
ALK ) . -
pubmed
Herein , we present the structure of
ALK in complex with
N1 -( 3 - 4 -[([ 5 -( tert - butyl )- 3 - isoxazolyl ] aminocarbonyl ) amino ]- 3 - methylphenyl-1H-5 - pyrazolyl )- 4 -[( 4 - methylpiperazino ) methyl ] benzamide ( 5a ) , a novel
ALK inhibitor adopting a type
II binding mode . -
pubmed
At baseline and at 4 - week intervals , blood lipids ,
C - reactive protein , and fasting blood
glucose were evaluated . -
pubmed
Consumption of PTE was associated with improvements to lipid profile , including a mild reduction in
cholesterol and the
cholesterol :
high - density lipoprotein ratio after only 4 weeks , as well as a reduction in
triglycerides and
very small - density lipoproteins , where average blood levels reached normal range at 8 weeks and remained within normal range for the duration of the study ( P<0.08 ) . -
pubmed
No significant changes between the PTE group and the placebo group were seen for fasting
glucose or
C - reactive protein . -
pubmed
On the other hand , the
bis ( acenaphthyl ) species show a fluxional behavior , resulting in an AX to
A2 spin system transition in the
( 31 ) P {( 1 ) H } variable - temperature NMR spectra . -
pubmed
BACKGROUND /
AIM :
5 - Aminolevulinic acid (
5 - ALA ) , a precursor of
protoporphyrin IX (
PpIX ) , is now used for photodynamic therapy ( PDT ) of pre -
cancers of the skin and photodynamic diagnosis ( PDD ) of
brain tumors . -
pubmed
The effect of the cafeteria diet for 8 wk , which also increases adipose tissue , or conjugated
linoleic acid (
CLA ) supplementation for 4 wk , a model of
lipodystrophy , was evaluated 24 h after knee challenge with mBSA . -
pubmed
Additional measures comprised factors of the
metabolic syndrome ,
cortisol , relative
insulin resistance , and pro - inflammatory
cytokines (
interleukin-6 ;
IL-6 and
tumor necrosis factor - alpha ,
TNF - alpha ) . -
pubmed
Further findings comprised increased fasting
cortisol , increased
TNF - alpha concentration , and decreased physical activity level in
MDD compared to
CTRL . -
pubmed
In addition to routine biochemical analysis , fasting blood samples were studied for
prothrombin time ( PT ) , activated partial
thromboplastin time ( aPTT ) ,
fibrinogen ,
protein - C ,
protein - S ,
antithrombin III ,
factor VII ,
factor VIII , D - dimer ,
vitamin B12 ,
folic acid and
homocysteine . -
pubmed
Levels of
fibrinogen , D - dimer ,
protein C ,
factor VIII , total
homocystein and the number of platelets were found to be significantly higher in
Crohn ' s disease patients who were in active period of the disease . -
pubmed
The relationship between serum
serotonin concentration and
depression is well known , but there are no enough data regarding the serum change of
leptin and
adiponectin . -
pubmed
The mean serum
serotonin level was significantly lower in the group with
PPD ( p = 0.001 ) , while mean serum
adiponectin level was higher ( p = 0.001 ) . -
pubmed
The key strategies for the construction of the molecular complexity in the targets included an asymmetric Tsuji - Trost rearrangement to set the first quaternary
carbon center at C20 , an intramolecular cyclopropanation by
diazo decomposition to install the second and third quaternary
carbon centers at
C2 and
C7 , respectively , and a
SmI2 -promoted
acyloin condensation to assemble the
isokopsine core . -
pubmed
Salicylic acid (
SA ) is an important plant hormone that regulates many aspects of plant growth and development , as well as resistance to ( a)biotic stress . -
pubmed
We therefore studied the influence of the surface chemistry of mesoporous
silica nanoparticles ( MSNs ) carrying identical amounts of a specific antibody (
anti - B220 ) on the selectivity toward
B220 - positive
leukemia stem cells . -
pubmed
A significant
ROR value ( higher than 1 ) was only found for
citalopram ( 3.35 CI95 % [ 2.90 - 3.87 ] ) or
escitalopram ( 2.50 [ 2.11 - 2.95 ] ) . -
pubmed
Herein , we demonstrate that the three - dimensional porous
polyacrylamide -
phytic acid -
polydopamine ( termed as
PAAM /
PA /
PDA ) hydrogel can be served as reusable adsorbent with high efficiency for either anionic or cationic dyes . -
pubmed
Using
methyl blue (
MB ) ,
methylene blue (
YMB ) ,
methyl violet ( MV ) and
neutral red ( NR ) as model dyes , we investigate the effect of pH , temperature , dye concentration , and PAAM /
PA /
PDA hydrogel mass on the adsorption . -
pubmed
Adsorption kinetic and thermodynamic analysis suggests that the dyes are adsorbed on the
PAAM /
PA /
PDA hydrogel through the strong pi - pi stacking and anion - cation interaction , and the adsorption process satisfies a pseudo - second - order model . -
pubmed
Furthermore , the free - standing
PAAM /
PA /
PDA hydrogel can be easily removed from water after adsorption process , and regenerated by adjusting solution pH values . -
pubmed
Characterization of
DNA ADP - ribosyltransferase activities of
PARP2 and
PARP3 : new insights into DNA
ADP - ribosylation . -
pubmed
Poly ( ADP - ribose ) polymerases (
PARPs ) act as DNA break sensors and catalyze the synthesis of polymers of
ADP -
ribose (
PAR ) covalently attached to acceptor proteins at DNA damage sites . -
pubmed
According to DNA substrate specificity of
PARP3 and
PARP2 , we propose a putative mechanistic model of
PARP - catalyzed strand break - oriented
ADP - ribosylation of DNA termini . -
pubmed
Notably ,
PARP - mediated DNA
ADP - ribosylation can be more effective than
PARPs ' auto -
ADP - ribosylation depending on the DNA substrates and reaction conditions used . -
pubmed
These results suggest that certain types of complex DNA breaks can be effectively
ADP - ribosylated by
PARPs in cellular response to DNA damage . -
pubmed
In addition , the ultrasound energy density affected the physical properties of the beverage as the size distribution of fat globule and rheological behavior , as well as the chemical properties such as antioxidant activity ,
ACE inhibitory activity ,
fatty acid profile , and volatile profile . -
pubmed
Synthesis and Characterization of
Pyridine Compounds for Amperometric Measurements of
Leukocyte Esterase . -
pubmed
Compound III had the lowest
octanol / water partition coefficient ( log p=0.33 ) along with the highest topological surface area ( tPSA=222
A2 ) and the best aqueous solubility ( 4.0 mg mL-1 ) . -
pubmed
UCA1 confers
paclitaxel resistance to
ovarian cancer through miR-129 /
ABCB1 axis . -
pubmed
The objective of this work was to investigate the role and molecular mechanisms of
urothelial carcinoma associated 1 (
UCA1 ) in
paclitaxel (
PTX ) resistance in
OC . -
pubmed
Collectively , our study elaborated a novel
UCA1 / miR-129 /
ABCB1 regulatory axis underlying
PTX resistance of
OC cells , providing a potential therapeutic target for
OC . -
pubmed
Among nonnatural
amino acids ,
selenocysteine is the catalytic group of
glutathione peroxidase (
GPx ) . -
pubmed
Exposed groups were treated with the
Hedgehog pathway antagonist
GDC-0449 at a single dose 150 mg / kg by oral gavage from E8.5 to E15.5 . -
pubmed
Post - transplant
RCC was significantly associated with longer durations of pre - transplant hemodialysis ( p = 0.007 ) and
post - transplant immunosuppression with
cyclosporine ( p = 0.029 ) and/or
mycophenolate mofetil ( p = 0.020 ) and with larger proportions of
post - transplant time on
mycophenolate mofetil ( p = 0.046 ) and/or
prednisolone medication ( p = 0.042 ) . -
pubmed
gingivalis inhibited
Idh1 / 2 and
Gpt1 / 2 mRNA expression , and increased
Akgdh mRNA expression , thus decreasing the ratio of
alpha - KG /
succinate . -
pubmed
Although the
H2A.Bbd -
H2B dimer structure largely resembles that of
H2A -
H2B , substitution of
H2A alphaC helix residues by
H2A.Bbd counterparts lead to the transition of a long alphaC - helix to the short 310 - helix , likely owing to the rearrangement of the
hydrogen - bond network . -
pubmed
The
plsC /
plsX - overexpressing ( OX + XC )
strain produced high lipid content of about 24.3%w / dcw under normal condition and was further enhanced up to 39.1%w / dcw by
acetate induction . -
pubmed
This OX + XC engineered
strain was capable of decreasing
phaA transcript level which related to
poly-3 - hydroxybutyrate (
PHB ) synthesis under
acetate treatment . -
pubmed
Moreover , the expression level of gene transcripts revealed that the
plsX- and
plsC /
plsX - overexpression
strains had also increased
accA transcript amounts which involved in the irreversible carboxylation of
acetyl - CoA to
malonyl - CoA. -
pubmed
Mirogabalin , which is a novel ligand for the
alpha2delta subunit of voltage - gated calcium channels , is under development for the treatment of
pain associated with
diabetic peripheral neuropathy and
postherpetic neuralgia . -
pubmed
Buprenorphine shows its pharmacological effects by binding to
opioid receptors . -
pubmed
Kinetics of the
Hydrogen Abstraction
PAH +
OH PAH Radical +
H2O Reaction Class : An Application of the Reaction Class Transition State Theory ( RC -
TST ) and Structure - Activity Relationship (
SAR ) . -
pubmed
A reaction class transition state theory ( RC -
TST ) augmented with structure - activity relationship (
SAR ) methodology is applied to predict high - pressure limit thermal rate constants for
hydrogen abstraction by
OH radical from
polycyclic aromatic hydrocarbons (
PAHs ) reaction class in the temperature range of 300 - 3000 K. The rate constants for the reference reaction of
C6H6 +
OH C6H5 +
H2O is calculated by the canonical variational transition state theory ( CVT ) with small curvature tunneling (
SCT ) . -
pubmed
After adjusting for potential confounders , age , living in urban areas ,
alcohol consumption , central
obesity , elevated fasting plasma
glucose level , elevated blood pressure , lower
high - density lipoprotein cholesterol level and elevated
triglycerides level were associated with increased risk of
HUA . -
pubmed
Background :
Omentin , releasing by adipose - tissue may be related to
glucose metabolism . -
pubmed
The present study involved the measurements of oral -
glucose - tolerance - test and the levels of biochemical parameters , including blood
glucose ,
omentin ,
insulin , lipid - profile , as well as
aminotransferases . -
pubmed
The plasma
omentin levels were significantly higher in
type-1 diabetes group and lower in
type-2 diabetes with
NPD +
STZ ; however , the plasma
omentin levels were not changed in the HFD +
STZ group . -
pubmed
Conclusions : The
omentin expression may be affected by
insulin and
glucose levels in different types of
diabetes more than fat - mass , and due to the local activity , the serum
omentin may not comply with its gene expression . -
pubmed
AIM : Previous reports showed associations between
oxytocin induced
labor and
mental disorders in offspring . -
pubmed
We investigated the neonatal
brain damage with evaluating immediate early gene expression (
c - Fos ,
c - Jun and
JunB ) by quantitative
polymerase reaction and TdT - mediated
dUTP nick end labeling staining . -
pubmed
To investigate the injured brain cell types , we performed double - immunostaining with TdT - mediated
dUTP nick end labeling staining and each brain component specific protein , such as
Oligo2 ,
NeuN ,
GFAP and
Iba1 . -
pubmed
Expression levels of
oxytocin and
Oxtr in the fetal brain were not affected by the
oxytocin administered to mothers during induced labor . -
pubmed
We also showed that C /
Co@
polyMAPTAC could reduce the
cytotoxicity of the
Abeta aggregate solutions . -
pubmed
In the
post - operative phase , therefore after the surgical treatment of mammary quadrantectomy , or mastectomy associated with homolateral sentinel lymph node removal + - axillary dissection , based on the histopathological characteristics of the
tumor , the use of chemotherapy (
anthracycline +
taxane ) and/or hormone therapy , whether or not associated with anti -
HER2 therapy (
trastuzumab ) is the rule . -
pubmed
In this work , the
cytotoxic behavior of six
ruthenium ( II ) complexes of stoichiometry
[ ( eta6 - p - cymene ) RuCl2L ] ( I - VI ) , L =
4 - cyanopyridine ( I ) ,
2 - aminophenol (
II ) ,
4 - aminophenol ( III ) ,
pyridazine (
IV ) , and
[ ( eta6 - p - cymene ) RuClL2 ] PF6 ; L =
cyanopyridine ( V ) , L =
2 - aminophenol(VI ) towards three cell lines was studied . -
pubmed
The MIM2 - NMR method yields a mean absolute deviation (
MAD ) from unfragmented full molecule calculations of 0.01 ppm for 1H and 0.06 ppm for
13C chemical shifts . -
pubmed
The 1H and 13C NMR chemical shifts calculated using our protocol give excellent agreement with experiments for two larger
proteins , 2MC5 ( the helical part with 265 atoms ) and
3UMK ( 33 residue slice with 547 atoms ) . -
pubmed
Overall , our target accuracy of ~0.3 ppm for 1H and ~2 - 3 ppm for
13C has been achieved for the larger
proteins . -
pubmed
The
enzyme was eluted either at pH 6.8 in a concentration gradient of
NADP+ , or at pH 8.5 in the presence of
NADP+ in concentration gradients of either
dicarboxylates or
NaCl . -
pubmed
2 '- AMP and its derivatives ,
8 - bromoadenosine 2 '- phosphate and
8 -( l - aminohexyl ) aminoadenosine 2 '- phosphate , which were used in the synthesis of the adsorbent , all acted as
enzyme inhibitors competitive with
NADP+ . -
pubmed
The chromatographic properties of the wild - type
enzyme were compared with those of mutationally modified variants containing defined
amino acid substitutions . -
pubmed
The adsorbent showed a low capacity for the
enzyme from mutant
am1 (
Ser-336 replaced by
Phe ) , a variant that has a localized defect in
NADP binding , but an otherwise almost normal conformation , suggesting that non - specific interactions are at most weak . -
pubmed
This is consistent with measurements in free solution that showed the
am3 enzyme to have a higher Ki for
2 '- AMP than the wild - type enzyme . -
pubmed
Methioninyl adenylate is a specific and potent inhibitor of the enzyme
methionyl - tRNA synthetase and , consequently , of protein biosynthesis . -
pubmed
The effect of
NMMA requires functional
MCP ,
cheA gene product ,
cheB gene product , and possibly
cheX gene product . -
pubmed
sudden dilution ) was detected by
cheZ mutants with much higher sensitivity than that of a conventional repellent ,
indole , while both signals were rather poorly but equally detected by
cheB mutants . -
pubmed
The pulmonary lymphatics were shown to play an important role in the
wash - out of the
contrast media . -
pubmed
Compared with that of the controls , the 24 - hour basal
insulin secretion of islets cultured in the presence of
cyproheptadine was significantly reduced from the 4th day of the study onwards . -
pubmed
Labeling of
CK8 / 18 was not inhibited by
tunicamycin , suggesting that glycosylation was not N - linked . -
pubmed
Acid hydrolysis of
CK8 and
CK18 , purified from
[ 3H ] glucosamine - labeled cells , generated free
glucosamine . -
pubmed
In the presence of
UDP -[ 3H ] galactose ,
galactosyltransferase catalyzed the labeling of
cytokeratin 8 and
18 . -
pubmed
beta - Elimination of the
[ 3H ] galactose- labeled
CK8/18 generated the
disaccharide N - acetyllactosaminitol , indicating that
cytokeratin 8 and 18 contain single
O - linked N - acetylglucosamine residues . -
pubmed
Using chemical analysis , the stoichiometry of glycosylation was found to be 1.5 and 2 molecules of
N - acetylglucosamine / protein molecule of
CK8 and
CK18 , respectively . -
pubmed
Peptide maps of
[ 3H ] glucosamine - labeled
CK8 / 18 showed that multiple peptides were labeled with the
amino sugar . -
pubmed
The biosynthetic and degradation rates of the
carbohydrate moiety were faster than the
protein core as determined by metabolic radiolabeling or
pulse - chase experiments , respectively . -
pubmed
Our results show that
CK8 and 18 are glycosylated at multiple sites with a single O - linked
N - acetylglucosamine . -
pubmed
M3 - subtype muscarinic receptor that controls intracellular
calcium release and
inositol phosphate accumulation in gastric parietal cells . -
pubmed
Other M2 - agonists ,
oxotremorine and
arecoline , produced a partial [
Ca2 +]i increase , whereas
M1 - agonists ,
pilocarpine and
[ 4 - m - chlorophenylcarbamoyloxyl ]- 2 - butynyl - trimethylammonium , were without any significant effect . -
pubmed
[
Ca2+]i rise was inhibited by selective
muscarinic antagonists :
atropine greater than
4 - diphenylacetoxy - N - methyl - piperidine methbromide greater than
quinuclidinylbenzilate (
QNB ) greater than
pirenzepine greater than
11 -[[ 2 -[( diethylamino ) methyl ]- 1 - piperidinyl ] acetyl ]- 5,11 - dihydro-6H - pyrido [ 2,3 - b ][ 1,4 ] benzodiazepine-6 - one , this sequence being characteristic of the involvement of an
M3 - subtype . -
pubmed
The IC50 values for inhibition of [
Ca2+]i increase by muscarinic antagonists were in good agreement with those obtained for inhibition of
phospholipase C activation . -
pubmed
Basal
oxygen consumption fell from
16.1 + /- 0.72 ( + /- SEM ) to 10.1 + /- 0.47 ml / min per kg between 4 and 55 days of age , while breathing frequencies fell from 52.3 + /- 4.4 to 32.4 + /- 1.6 breaths / min over this period . -
pubmed
Inhibition of
neural phospholipase D activity by
aminoglycoside antibiotics . -
pubmed
These results indicate that
aminoglycoside antibiotics inhibit
phosphatidylcholine - PLD activity with comparable and sometimes greater potency than their well known inhibition of
phosphoinositide - phospholipase C. -
pubmed
The possibility that
PLD inhibition could mediate some of the toxic side effects of
aminoglycosides is suggested . -
pubmed
The increase of activity can be inhibited by administering the selective
dopamine D1 receptor agonist
SKF 38393 , but not the selective
D2 agonist
quinpirole , or
apomorphine . -
pubmed
Conversely , in the dark , enzyme activity can be enhanced by administering the selective
D1 antagonist
SCH 23390 or
haloperidol , but not the selective
D2 antagonist
( -)- sulpiride . -
pubmed
Kinetic analysis indicated that the apparent Vmax for the
enzyme increases with little change in the apparent Km for the substrate
3,4 - dihydroxyphenylalanine or the cofactor
pyridoxal-5 '- phosphate . -
pubmed
We suggest that
dopamine released in the dark tonically occupies
D1 receptors and suppresses AAAD activity . -
pubmed
The algorithm involved identification of ( a ) secondary
peritonitis associated with
gut perforation , based on previously proposed criteria in patients with
neutrocytic ascites ( ascitic fluid
total protein greater than 1 g / dl ,
glucose less than 50 mg / dl , and
lactate dehydrogenase greater than the upper limit of normal for serum ) and ( b ) separation of spontaneous from secondary
peritonitis ( unassociated with
perforation ) based on the response of the ascitic fluid cell count to antibiotic therapy . -
pubmed
Pentoxifylline has been shown to decrease endotoxin - induced
tumor necrosis factor alpha production and reverse the inflammatory actions of
interleukin-1 (
IL-1 ) and
tumor necrosis factor on leukocyte function . -
pubmed
Additionally ,
pentoxifylline attenuated meningeal inflammatory changes induced by intracisternal inoculation of 10 ng of
rabbit recombinant IL-1 beta compared with results in either
dexamethasone- or saline - treated animals . -
pubmed
In addition ,
periodate treatment of the
24,25 -( OH ) 2 - D3 product resulted in complete loss of the product as measured by
protein - binding assay . -
pubmed
Activity of the
24 - hydroxylase in the crude solubilized extract was
6.3 pmol / h per mg , but was undetectable after partial purification by a
p - chloroamphetamine - Sepharose column . -
pubmed
However , further fractionation of this material by
DEAE - Sepharose chromatography resulted in a further increase in
1 alpha - hydroxylase activity to 430 pmol / h per mg and detection of
24 - hydroxylase in a separate fraction at a level of 53 pmol / h per mg . -
pubmed
Production of
1,25 -( OH ) 2 - D3 was linear with time up to 2 h and was dependent upon
ferredoxin concentration as well as
cytochrome P-450 concentration in the range of 0 - 40 nM. -
pubmed
In the presence of excess
ferredoxin and adequate amounts of
cytochrome P-450 ,
1,25 -( OH ) 2 - D3 production was also dependent upon substrate concentrations in the range of 0.25 to 2.5 microM yielding an estimated Km of 1 microM. -
pubmed
This transcription appeared 2 to 4 h after addition of
DMSO when expression of the c -
myc gene was downregulated by blocking RNA elongation at the end of exon 1 . -
pubmed
The formation of
retinoyl - beta - glucuronide is catalyzed by a
UDP - glucuronosyltransferase with apparent Km 's of 54.7 microM for
all - trans - retinoic acid and 2.4 mM for
UDP - glucuronic acid . -
pubmed
The specific activity of the
enzyme is increased by
vitamin A deficiency . -
pubmed
The adult
lactase phenotype ,
lactose absorber or malabsorber , was determined using the
lactose tolerance test with breath
hydrogen assay in a group of Tuareg , a traditionally nomadic pastoralist population in the central Sahara . -
pubmed
AQ -
AH 208 produced a significant ( P less than 0.05 ) increase ( 35 % ) in transmural collateral perfusion whereas
propranolol had no effect . -
pubmed
Certain growth factors ( such as
epidermal growth factor ,
platelet - derived growth factor , and
transforming growth factors - alpha and - beta ) have been shown to stimulate the resorption of bone in organ culture by both
prostaglandin - dependent and
prostaglandin - independent pathways . -
pubmed
The in vivo
calcium - mobilizing activity of
epidermal growth factor and
transforming growth factor - alpha indicate that these or related growth factors need be considered as potential mediators of
tumor - induced
hypercalcemia . -
pubmed
The reduced
dexamethasone - suppressibility of plasma
cortisol , which is characteristic of pregnancy , extends into the
post - partum period . -
pubmed
Antagonistic effects of
apomorphine and
haloperidol on
rat striatal synaptosomal tyrosine hydroxylase . -
pubmed
Stereospecificity of the oxidation of
ethanol by
catalase . -
pubmed
[ Urinary
phenolic acids during
rest and muscular activity ] . -
pubmed
We have investigated the mechanisms of blockade of
calcium channel current by the
dihydropyridines , e.g. -
pubmed
Our results show that
calcium channel blockade by
dihydropyridine derivatives is strongly modulated by membrane potential . -
pubmed
Our results suggest that
dihydropyridines bind preferentially to the inactivated state of the
calcium channel , and that the development of use - dependent block is related to the ionization constants of the compounds . -
pubmed
This antisecretory action was blocked by
naloxone and not mediated via an effect on tissue
cyclic AMP level or
adenylate cyclase activity . -
pubmed
The first set of observations suggest that
loperamide 's antisecretory effect is mediated via
opiate receptors either distal to , or separate from the
adenylate cyclase /
cyclic AMP pathway . -
pubmed
Release of
sialic acid from substrates by
sialidase in the presence of
H2 [ 18O ] . -
pubmed
All three
kinases had molecular weights between 70 000 and 75 000 which increased to 170 000 - 200 000 in the presence of 2 mM
ATP . -
pubmed
Studies on the kinetic properties of the
enzymes with
thymidine and
ATP as the substrates and
dTTP as the inhibitor showed clear differences between
TK 1 and
TK 2 , but a close similarity between
TK 2 and TK 2N. -
pubmed
With
thymidine as the variable substrate ,
TK 1 showed Michaelis - Menten kinetics , whereas
TK 2 and TK 2N showed characteristic biphasic kinetics . -
pubmed
With
ATP as the variable substrate , all three
enzymes showed positive cooperative kinetics , but
TK 2 and TK 2N lost the cooperativity in the presence of
dTTP . -
pubmed
The results from inhibition studies showed , that
dTTP was a cooperative inhibitor of
TK 1 but a non - cooperative inhibitor of
TK 2 and TK 2N. -
pubmed
Ultrapure populations of G1- , S- , and
G2 - M - phase cells were obtained through sequential centrifugal elutriation and flow cytometric cell sorting of
Hoechst 33342 - stained cells . -
pubmed
Angiotensin II (
ANG II ) and
NaCl act on central receptors to cause pressor responses that may be mediated in part by the sympathetic nervous system . -
pubmed
These findings illustrate that stimulation of
sodium - sensitive and
angiotensin - sensitive CNS receptors produces differential responses in sympathetic outflow . -
pubmed
Inactivation of purified
rat liver cytochrome P-450 by
chloramphenicol . -
pubmed
BACKGROUND & amp ; AIMS :
Platelet - activating factor (
PAF ) is a potent inflammatory mediator implicated in the pathogenesis of
inflammatory bowel disease and
necrotizing enterocolitis . -
pubmed
Metabolism by
platelet - activating factor acetylhydrolase (
PAF - AH ) is the major pathway for
platelet - activating factor degradation . -
pubmed
Both intracellular and secreted
PAF - AHs are
calcium - independent and substrate - specific for
phospholipids similar to
PAF . -
pubmed
Nuclear multicatalytic
proteinase alpha subunit
RRC3 : differential size ,
tyrosine phosphorylation , and susceptibility to antisense
oligonucleotide treatment . -
pubmed
NS RRC3 showed a significantly smaller M(r ) ( 24,000 ) than the cytoplasmic form ( M(r ) 28,000 ) , and only the nuclear form contained
phosphotyrosine . -
pubmed
In metabolic labeling experiments with
[ 32P ] orthophosphate , the major nuclear and
NS form observed showed an M(r ) of 24,000 , whereas no labeling of cytosolic
RRC3 was observed . -
pubmed
A minor
32P - labeled band of M(r ) 28,000 was also observed in nuclei , and this M(r ) 28,000 form was found in the soluble nuclear extract within
MCP complexes . -
pubmed
These results suggest that
tyrosine phosphorylation of the cytosolic form ( M(r ) 28,000 ) rapidly triggers nuclear import , which is in turn quickly followed by conversion to the major M(r ) 24,000 form associated with
NS . -
pubmed
ECL cells store
histamine and are considered a key element in the mechanisms of gastric acid secretion as controlled by
gastrin stimulus . -
pubmed
Kinetic analysis of expressed transport activity revealed a saturable transport system shared by
amino beta - lactam antibiotics ,
dipeptides and selected
angiotensin converting enzyme inhibitors . -
pubmed
The present data indicate that
PAMP as well as
adrenomedullin is processed from an
adrenomedullin precursor . -
pubmed
The recommended dose for Phase
II studies is 80 mg m-2 of
CPT-11 and 60 mg m-2 of
cisplatin . -
pubmed
The charge density of renal interstitium was analysed from the volume of distribution of negative native
albumin as compared with neutralized
albumin , labelled with 125I and
131I , respectively . -
pubmed
The results of kinetic studies of the
enzyme activity indicated that
ascorbic acid /
Fe ( 2 +)-induced inhibition of the
enzyme activity is mainly due to an increased Km value for the substrate . -
pubmed
A decreased activity of the
microsomal G-6 - Pase was also observed when the microsomes were incubated with
aldehydes such as
malondialdehyde ,
n - heptaldehyde ,
acetaldehyde , and
trans-2 - nonenal . -
pubmed
Glucose-6 - phosphate (
G-6 - P)effectively prevented
ascorbic acid /
Fe ( 2 +)- or
trans-2 - nonenal - induced inhibition of the
enzyme activity , but the substrate failed to protect the protein
thiols in both systems . -
pubmed
The results of fluorescence anisotropy measurements of
diphenylhexatriene - labeled microsomes suggested that changes in the lipid dynamics are not directly related to peroxidation- mediated inhibition of the
enzyme activity . -
pubmed
Based on these results , a possible reason for the inhibition of the microsomal
G-6 - Pase activity associated with
ascorbic acid /
Fe ( 2 + ) treatment is discussed . -
pubmed
In T cells , overexpression of
wild - type SLP-76 results in a hyperactive receptor , while expression of a
SLP-76 molecule that is unable to be
tyrosine - phosphorylated attenuates receptor function . -
pubmed
In addition , the
SH2 domain of
SLP-76 is required for
T - cell receptor function , although its role is independent of the ability of
SLP-76 to undergo
tyrosine phosphorylation . -
pubmed
As
SLP-76 interacts with both
Grb2 and
phospholipase C - gamma1 , these data indicate that phosphorylation of
SLP-76 by
ZAP-70 provides an important functional link between the
T - cell receptor and activation of
ras and
calcium pathways . -
pubmed
The heart failed to generate contractile force and increased the resting tension markedly within 5 min of perfusion with
xanthine plus
xanthine oxidase . -
pubmed
The protective effects of
vanadate on the loss of developed tension and increased resting tension due to
xanthine plus
xanthine oxidase were dose - dependent ( 0.1 - 5 microM ) . -
pubmed
The
sarcolemmal Ca ( 2 +)- pump (
ATP - dependent
Ca2 + uptake and
Ca ( 2 +)- stimulated ATPase ) and Na
(+)-dependent
Ca2 + uptake activities were decreased upon perfusing the hearts with a medium containing
xanthine plus
xanthine oxidase for 5 min ; these effects were prevented by the addition of 2 - 4 microM
vanadate in the perfusion medium . -
pubmed
The signals for
superoxide radicals produced by
xanthine plus
xanthine oxidase , as detected by electron paramagnetic resonance spectroscopic technique , were inhibited by 5 - 100 microM
vanadate . -
pubmed
This reduced conductance was reflected in a 35 % decrease in binding of radiolabeled
saxitoxin , a
sodium channel - specific ligand , indicating expression of fewer channels in these neurons . -
pubmed
Thus , the altered regulation of voltage - gated
sodium channels in Ts16 neurons is apparently a
post - transcriptional event and possible mechanisms are discussed . -
pubmed
In the present study , we have examined , in vitro , the direct effects of
vinblastine (
VBL ) ,
5 - fluorouracil (
5 - FU ) ,
melphalan (
L - PAM ) and the novel
tubulin inhibitor
combretastatin A-1 (
CBS ) on endothelial permeability under normal and tumour simulated conditions . -
pubmed
The changes induced by
VBL and
CBS were accompanied by contraction of the endothelial
F - actin cytoskeleton . -
pubmed
Both
VBL and
CBS have vascular components in their mode of action which may lead to vascular collapse and
tumour necrosis . -
pubmed
In addition to a
TNF - alpha - mediated pathway , there is growing evidence that some particles such as
quartz and
crocidolite can directly activate lung epithelial cells to release
chemokines such as
macrophage inflammatory protein-2 ,
cytokine - induced neutrophil chemoattractant , and
interleukin-8 . -
pubmed
Six mutants , called LT7 K (
Arg to
Lys ) , LT61F (
Ser to
Phe ) , LT112 K (
Glu to
Lys ) , LT118E (
Gly to
Glu ) , LT146E (
Arg to
Glu ) and LT192 G (
Arg to
Gly ) were constructed by the replacement of one
amino acid at one position of the
A1 subunit to another using site - directed mutagenesis . -
pubmed
Importantly , the
rBAT 1.1 cDNA was derived from a 7.5 - kb messenger RNA ( mRNA ) and did not encode a functional
selenoenzyne unless an enabling selenocysteine insertion sequence was appended to the presumed coding region and this cDNA . -
pubmed
Direct analysis of the translation products programmed by the native
SeD2 mRNA in
cAMP - stimulated astrocytes was performed using antisense
deoxynucleotides and hybrid selection strategies . -
pubmed
However , no immunoreactive 30 - kDa
SeD2 protein was identified by Western analysis , immunoprecipitation , or immunocytochemistry , and the specific C - terminus antiserum failed to immunoprecipitate
deiodinase activity from
( Bu ) 2cAMP - stimulated astrocytes , brown adipose tissue or brain . -
pubmed
Instead , the native 7.5 - kb
SeD2 mRNA encoded a 15 - kDa protein that terminated at the first UGA codon and contained the catalytically inactive , N - terminal 129
amino acids of
SeD2 . -
pubmed
On the other hand , testing the
tyrosine phosphorylation of
FAK , revealed significant increases in
diabetes . -
pubmed
This demonstrated that the amounts of
FAK co - precipitated with
beta1 , as well as its
tyrosine - phosphorylation , are in fact reduced in
diabetic conditions . -
pubmed
The
bcl-2 transgene was strongly expressed in these cells , and resulted in protection of neuronal cultures from
death triggered by serum deprivation or exposure to
staurosporine . -
pubmed
Although specific DNA lesions that involve base loss or modification stimulate nucleic acid cleavage mediated by
type II topoisomerases , the effects of
deoxyribose sugar ring modification on enzyme activity have not been examined . -
pubmed
Therefore , the effects of incorporated
araC residues on the DNA cleavage / religation equilibrium of
human topoisomerase IIalpha and beta were characterized . -
pubmed
These findings broaden the range of DNA lesions known to alter
topoisomerase II function and raise the possibility that this enzyme may mediate some of the cellular effects of
araC. -
pubmed
Gastric antral G - cells process
progastrin at
Arg(94)Arg(95 ) and
Lys(74)
Lys(75 ) residues generating
gastrin heptadecapeptide (
G17 - NH(2 ) ) . -
pubmed
Conversely , duodenal G - cells process
progastrin to
gastrin tetratriacontapeptide ( G34 - NH(2 ) ) with little processing at
Lys(74)
Lys(75 ) . -
pubmed
Although mutation of the
Lys(74)
Lys(75 ) site within G34 - NH(2 ) to
Lys(74)Arg(75 ) resulted in the production of primarily
G17 - NH ( 2 ) rather than
G34 - NH ( 2 ) , inhibition of
PC1 /
PC3 did not significantly inhibit processing at the
Lys(74)Arg(75 ) site . -
pubmed
Endothelin-1 (
ET-1 ) is a 21
amino acid peptide that binds to
G - protein - coupled receptors to evoke biological responses . -
pubmed
ET-1 , but not
angiotensin - II (
A - II ) , stimulated
glucose uptake in a dose - dependent manner with an EC50 value at approximately 1 nM , and an approximately 2 - fold stimulation at 100 nM. -
pubmed
As a comparison ,
insulin stimulated
glucose uptake in a dose - dependent manner with an EC50 value at 1 nM , and a 2.5 - fold stimulation at 100 nM. -
pubmed
The effect of
ET-1 on
glucose uptake was blocked by
A-127722 , an antagonist selective for the
ET ( A )- receptor . -
pubmed
The effect of
ET-1 on
glucose uptake was not inhibited by inhibitors for
protein kinase C (
PKC ) ,
protein kinase A (
PKA ) and
phosphatidylinositol-3 - kinase (
PI3 '- kinase ) . -
pubmed
Aquaporin-2 (
AQP-2 ) is the
vasopressin - regulated
water channel expressed in the apical membrane of principal cells in the collecting duct and is involved in the urinary concentrating mechanism . -
pubmed
In addition , these results support the idea that
AQP-2 , and probably other
aquaporins , are involved in
water absorption in the colon . -
pubmed
The plasma
lactate , A and NA were analysed at
rest ( La0 , A0 and NA0 ) , immediately at the end of the exercise ( Amax and NA(max ) ) and after 5 min recovery (
La(max ) , A5 and NA5 ) . -
pubmed
Cell surface biotinylated
MPR and
( 125 ) I - labeled
transferrin are accumulated in
cellugyrin - positive vesicles and to a lesser extent in
cellugyrin - negative vesicles . -
pubmed
An average
cellugyrin - positive vesicle carries not more than one molecule of either
Glut4 ,
insulin - responsive aminopeptidase (
IRAP ) , or
transferrin receptor (
TfR ) , whereas
cellugyrin - negative vesicles contain five to six molecules of
Glut4 , more than 10
molecules of
IRAP , and still one molecule of
TfR per vesicle . -
pubmed
Thus hepatic
glucose production is depressed after LPS administration , which is , at least in part , due to the downregulation of
phosphoenolpyruvate carboxykinase (
PEPCK ) expression . -
pubmed
Nucleotide competition assays show the
kinase to be
ATP specific . -
pubmed
Cystathionine - beta - synthase (
CBS ) catalyzes the condensation of
serine and
homocysteine to form
cystathionine , an intermediate step in the synthesis of
cysteine . -
pubmed
The lipopolysaccharide - induced production of
IL-1 ,
TNF and
NO by peritoneal macrophages was significantly reduced . -
pubmed
Furthermore ,
RSG significantly recovered the
picryl chloride - induced delayed - type
hypersensitivity to almost normal levels from the higher or lower levels induced by different treatments of
cyclophosphamide with a normalization of
CD4 /
CD8 ratio . -
pubmed
This study was designed to compare measurements of cardiac output as produced by the partial
CO ( 2 ) rebreathing ( NICO ) , bolus (
BCO ) , and continuous thermodilution (
CCO ) methods of monitoring cardiac output . -
pubmed
[
Phosphotransferase action of
phosphomonoesterase in hepatic metabolism of
nucleosides ] . -
pubmed
Biological concentration by killer
clams of
cobalt-60 from radioactive fallout . -
pubmed
The activity of the mutant
p53 in
tumor cells is restorable based on the fact that
PAb241 antibody against the
carboxy - terminus of
p53 and peptides corresponding to the
p53 carboxy - terminus can restore specific DNA - binding ability to some mutant
p53 proteins . -
pubmed
High throughout screening of chemical libraries has led to the identification of a group of small synthetic molecules such as
CP-31398 , which can restore
p53 function to mutant
p53 by stabilizing the active conformation of the protein that is destabilized in many mutants . -
pubmed
Further understanding of the mechanisms by which
CP-31398 and
PRIMA-1 restore
p53 activity may not only lead to discovery of more potent analogs but may also suggest new strategies for
p53 - targeting in
tumor therapy . -
pubmed
OBJECTIVE :
11 beta - hydroxysteroid dehydrogenase type 1 (
11 beta HSD1 ) converts inactive
cortisone to active
cortisol . -
pubmed
RESULTS : Prior to
GH , the ratio Fm / Em was greater with
hydrocortisone compared with
cortisone acetate replacement ( 1.17 + /- 0.28 and 0.52 + /- 0.09 respectively , P & lt ; 0.001 ) or with normal subjects ( normal males : 0.81 + /- 0.24 , females 0.66 + /- 0.14 ) . -
pubmed
Conversely , the ratio of urinary free
cortisol /
cortisone did not change indicating unchanged
11 beta HSD2 activity . -
pubmed
Pioglitazone reduced the expression of
TIMP-1 and
TIMP-2 mRNA without changing
MMP-13 mRNA expression compared to the liver fed a CDAA diet alone . -
pubmed
In vitro ,
pioglitazone prevented the activation of hepatic stellate cells resulting in reducing the expression of
type I procollagen ,
MMP-2 ,
TIMP-1 , and
TIMP-2 mRNA with increased
MMP-13 mRNA expression . -
pubmed
Glutamate racemase (
MurI ) catalyzes the racemization of
glutamate ; two
cysteine residues serve as catalytic acid and base . -
pubmed
In what we term substrate flipping ,
Glu suddenly rotates into a binding pattern that resembles the
post - racemization state of the other enantiomer , i.e. -
pubmed
Peroxidative aggregation of
alpha - synuclein requires
tyrosines . -
pubmed
Although it is known that peroxidative chemistry leads to the aggregation of
alpha - synuclein in vitro , the specific
amino acid types of
alpha - synuclein involved in this type of aggregation have not been identified . -
pubmed
ABCA1 has been established to be required for the efflux of
cholesterol and
phospholipids to
apolipoproteins such as
apoA - I. -
pubmed
At present , it is unclear whether
ABCA1 - mediated lipid exposure is specific with regard to lipid headgroups and whether it requires
calcium activation and the presence of a lipid acceptor . -
pubmed
By using
C6 - N -( 7 - nitrobenz-2 - oxa-1,3 - diazol-4 - yl ) (
NBD)-labeled phospholipid analogues , we observed elevated redistribution of
phosphatidylserine and
phosphatidylethanolamine but not of
phosphatidylcholine analogues from the cytoplasmic to the exoplasmic leaflet of the plasma membrane of
ABCA1 - GFP expressing cells . -
pubmed
Whereas
glyburide affected neither the level of exofacial endogenous
PS nor the outward movement of the
amino phospholipid analogues , the latter was sensitive to intracellular
Ca2 + in
ABCA1 - GFP expressing cells , further enhancing outward analogue redistribution with respect to control cells . -
pubmed
Glyburide raised the level of receptor - mediated endocytosis in the
ABCA1 - GFP expressing cell to the level of control cells in the absence of
glyburide . -
pubmed
We report that
TA ( L ) activity participates in the control of
PAF levels in monocytes and macrophages and that its regulation differs in these two types of cells . -
pubmed
When 12 PBH was chosen as the time point for in vitro intervention with the application of specific
NF - kappaB inhibitor
pyrrolidine dithiocarbamate (
PDTC ) ,
PKC inhibitor H-7 and PNS , both TNF - alpha mRNA expression and
NF - kappaB activity decreased significantly . -
pubmed
This child is alive and well
today because of these well - trained
camp counselors . -
pubmed
Circulating total
testosterone and
insulin - like growth factor - I (
IGF - I ) experienced significant ( P & lt ; 0.05 ) declines , and
cortisol was significantly increased . -
pubmed
IGF - I and
cortisol , but not total
testosterone , were correlated with losses of tissue mass . -
pubmed
With severe
weight loss ( & gt;or=13 % of body mass ) ,
IGF - I and
cortisol correlate more closely with soft - tissue tissue adaptations than does
testosterone . -
pubmed
Serum
25 - OH - vitamin D was 18.2ng / ml ( normal , 20 - 40ng / ml ) and
serum parathyroid hormone was 620pg / ml ( normal , 15 - 65pg / ml ) , suggesting
secondary hyperparathyroidism . -
pubmed
Although the physiological role of
CP is uncertain , its role in
iron (
Fe ) homeostasis and protection against free radical - initiated cell
injury has been widely documented . -
pubmed
Previous studies showed the existence of two molecular isoforms of
CP : secreted
CP (
sCP ) and a membrane
glycosylphosphatidylinositol ( GPI)-anchored form of
CP (
GPI - CP ) . -
pubmed
Also , expression of
CP in huPBL is demonstrated by immunofluorescence with confocal microscopy and flow cytometry analysis using cells isolated from healthy blood donors with normal
Fe status . -
pubmed
In this context , the involvement of lymphocyte - derived
CP on host defense processes via its anti / prooxidant properties is proposed , giving further support for a close functional interaction between the immune system and the
Fe metabolism . -
pubmed
Hydrodynamic characterization of the
SufBC and
SufCD complexes and their interaction with fluorescent
adenosine nucleotides . -
pubmed
Genetic experiments in some organisms found that the
ATPase SufC is essential , though its role in
Fe - S biogenesis remains unclear . -
pubmed
Both
SufBC and
SufCD bound
mantATP and
mantADP much more tightly than does
SufC alone . -
pubmed
Compared with
heparin plus
GPI ,
bivalirudin monotherapy resulted in similar protection from
ischemic events with less major
bleeding . -
pubmed
Compared with treatment with
heparin plus
GPI ,
bivalirudin monotherapy provides similar protection from
ischemic events with less major
bleeding , resulting in a significant reduction in net adverse clinical outcomes . -
pubmed
Mutation on
Ikappa kinase beta cysteine 179 markedly diminishes the effect of A(4)-NP , suggesting that A(4)-NP acts via
thiol modification at this residue . -
pubmed
Accordingly , the effects of A(4)-NP are independent of
peroxisome proliferator - activated receptor - gamma and are dependent on an intact reactive
cyclopentenone ring . -
pubmed
Thus , we have demonstrated that A(4)/J(4)-NPs , formed via the oxidation of
DHA , are potent inhibitors of
NF - kappaB signaling and may contribute to the anti - inflammatory actions of
DHA . -
pubmed
The results obtained were as follows : an extraction yield from 2.64 to 4.69 g/100 g ; an
AGA content between 49.8 and 64.06 g/100 g ; a content of
metoxil between 4.72 and 7.18 g/100 g ; a degree of esterification between 37.94 and 52.20 % ; an equivalent weight from 385.47 to 464.61 g / equivalent of H+ , and a degree of gelation between 28.64 and 806.03 g force . -
pubmed
Activities of
tryptophan decar - boxylate (
TDC ) and
anthranilate synthase (
AS ) , two
enzymes In the pathway leading to
ajmalicine , were higher after induction with 40 g / L
glucose than after induction with 60 or 80 g / L
glucose . -
pubmed
Experiments with different media containing mixtures of
glucose and the nonpermeating osmotic agent
xylose , and using an already induced culture as inoculum , revealed that a minimum amount of
glucose is required to support
ajmalicine production after
enzyme induction . -
pubmed
Differences in
enzyme induction and
ajmalicine production at different
glucose levels could not be explained by the intracellular concentrations of
glucose ,
fructose ,
sucrose , or starch . -
pubmed
Older antihypertensive drugs ( diuretics , beta - blockers , centrally acting ) exert negative results , whereas newer drugs have either neutral (
calcium antagonists ,
angiotensin - converting enzyme inhibitors ) or beneficial effects (
angiotensin receptor blockers ) . -
pubmed
Most of the
calcium that activates cardiac contraction comes from the sarcoplasmic reticulum (
SR ) from where it is released through the
RyR. -
pubmed
The amplitude of the systolic
Ca transient depends steeply on the
SR Ca content and it is therefore important that
SR content be regulated . -
pubmed
This regulation occurs via changes of
SR Ca content affecting systolic
Ca and thence sarcolemmal
Ca fluxes . -
pubmed
The main part of the review considers the effects of modulating the
RyR on systolic
Ca . -
pubmed
Potentiation of
RyR opening produces an increase of the amplitude of the
Ca transient but this effect disappears within a few beats because the increased sarcolemmal efflux of
Ca decreases
SR Ca content . -
pubmed
Some
cardiac arrhythmias result from release of
Ca from the
SR in the form of waves . -
pubmed
This is best known to occur when the
SR is overloaded with
calcium . -
pubmed
Mutations in the
RyR also produce
cardiac arrhythmias attributed to
Ca waves due to leaky
RyRs and a similar leak has been suggested to contribute to
arrhythmias in
heart failure . -
pubmed
We show that , due to compensatory changes of
SR Ca content , simply making the
RyR leaky does not produce
Ca waves in the steady state and that
SR Ca content is critical in determining whether
Ca waves occur . -
pubmed
AIM : The aim of this study was to determine if topical
atropine , used to retard axial length elongation and
myopia progression , had any effect on
ocular astigmatism . -
pubmed
AR mutants bind promiscuous
steroids , and may convert
AR antagonists to agonists . -
pubmed
Various non - hormonal growth factor receptors transactivate the
AR , even in absence of
androgens ( outlaw pathway ) . -
pubmed
Degenerating
somas ( neuronal
death ) and axonal / synaptic endings ( terminal degeneration ) were depicted by a
silver technique , and functionally affected cortical neuronal subpopulations by
Egr-1 ,
c - Fos , and
FosB /
DeltaFosB - immunolabeling . -
pubmed
In intact males ,
MK801 triggered a
c - Fos induction that remained high for more than 24 h in selected layers of the retrosplenial , somatosensory and entorhinal cortices . -
pubmed
MK801 - induced
neurodegeneration reached its peak at 72 h. Degenerating
somas were restricted to layer
IV of the granular subdivision of the retrosplenial cortex , and were accompanied by suppression of
Egr-1 immunolabeling . -
pubmed
Induction of
FosB /
DeltaFosB by
MK801 also extended to the same cortical layers affected by terminal degeneration , likely reflecting the damage of synaptic connectivity . -
pubmed
Degenerative
somas in layer
IV of the retrosplenial cortex significantly increased , with a parallel enhancement of terminal degeneration and
FosB /
DeltaFosB - expression in the mentioned cortical structures , but no additional areas were affected . -
pubmed
This article aims to focus on the effect of
H ( 2 ) O ( 2 )-redox homeostasis on hair follicle pigmentation via
tyrosinase , its substrate supply and signal transduction as well as the role of
methionine sulfoxide repair via
methionine sulfoxide reductases A and B (
MSRA and B ) . -
pubmed
PPBD was mapped to the highly conserved 54
amino acids at the C - terminal end of
tubby and
Tulp1 . -
pubmed
In addition , exercise disrupted the clustering of
cytokine expression and improved
glucose tolerance , without reducing body fat or
cytokine levels . -
pubmed
Exercise improves
glucose tolerance and disrupts the coexpression of proinflammatory
cytokines , suggesting that increased aerobic exercise may act independently of
weight loss in promoting joint health . -
pubmed
RESULTS : Le
antigen phenotyping revealed a significant ( p & lt ; .0001 ) association between type 1 (
Le ( a ) and
Le ( b ) ) expression and
strains of East Asian origin . -
pubmed
Tyrosine ammonia lyase catalyzes the deamination of
L : - tyrosine to
trans - coumaric acid . -
pubmed
The disruption of the
bagA gene abolished
trans - coumaric acid and
bagremycins production . -
pubmed
Thus ,
trans - coumaric acid is a precursor for biosynthesis of
bagremycins and the
bagA gene codes for
tyrosine ammonia lyase to synthesize
trans - coumaric acid . -
pubmed
A hypothalamic neuropeptide ,
gonadotropin - releasing hormone (
GnRH ) , is the primary factor regulating
gonadotropin secretion . -
pubmed
An inhibitory hypothalamic neuropeptide for
gonadotropin secretion was , until recently , unknown , although gonadal sex
steroids and inhibin can modulate
gonadotropin secretion . -
pubmed
GnIH acts on the pituitary and on GnRH neurons in the hypothalamus via a novel
G protein - coupled receptor (
GPR147 ) . -
pubmed
GnIH decreases
gonadotropin synthesis and release , inhibiting gonadal development and maintenance . -
pubmed
More recent evidence suggests that
GnIH also acts both upstream of the
GnRH system and at the level of the gonads to appropriately regulate reproductive activity across the seasons and during times of stress . -
pubmed
Tumor cell repopulation was quantified by
bromodeoxyuridine incorporation and
Ki67 by immunohistochemistry and/or flow cytometry . -
pubmed
In vitro cell killing was determined by classic
chromium - released assay , and reverse transcription PCR ( RT - PCR ) was carried out to determine the gene expression of associated
cytokines . -
pubmed
Tumor cell repopulation during the intervals of
cisplatin was inhibited by
CTLA-4 blockade . -
pubmed
When comparing roots of fully
sulfate - starved plants with
sulfate - starved split - root roots , expression of several potentially
OAS responsive genes was attenuated in split - roots depending on the shoot
sulfate status and the local root
O - acetylserine concentration . -
pubmed
In contrast , high - affinity
sulfate transporters displayed similar expression in
sulphate - starved split - roots and the corresponding controls . -
pubmed
Sucrose preference was not observed in
TS group . -
pubmed
Moreover , patients with constitutive
IFN - stimulated gene ( ISG ) expression have a poor response to treatment with pegylated
IFN - alpha (
PegIFN - alpha ) and
ribavirin . -
pubmed
HLA - DP and
IL28B polymorphisms : influence of host genome on
hepatitis B surface antigen seroclearance in
chronic hepatitis B. -
pubmed
BACKGROUND : The roles of single - nucleotide polymorphisms ( SNPs ) at
HLA - DP and
IL28B loci on
hepatitis B surface antigen (
HBsAg ) seroclearance in
chronic hepatitis B ( CHB ) infection are unknown . -
pubmed
METHODS : We compared the
HLA - DP ( rs3077 , rs9277378 , rs3128917 ) and
IL28B ( rs12979860 , rs8099917 ) polymorphisms of 203
CHB patients achieving spontaneous
HBsAg seroclearance with 203 age- and sex - matched
CHB patients without
HBsAg seroclearance ( controls ) . -
pubmed
HLA - DP rs3077 was associated with
HBsAg seroclearance in terms of allelic frequency ( minor allele A vs major allele G , P = .035 ; odds ratio [ OR ] , 0.699 ; 95 % confidence interval [ CI ] , .501-.976 ) and genotypic frequency ( AA vs GG /
GA , P = .014 ; OR , 0.295 ; 95 % CI , .106-.822 ) . -
pubmed
Haplotype analysis of
HLA - DP polymorphisms showed haplotype block GAT ( rs3077 / rs9277378 / rs3128917 ) to be associated with
HBsAg seroclearance ( OR , 2.17 ; 95 % CI , 1.06 - 4.45 , P = .034 ) . -
pubmed
Influence of
HLA - DP polymorphisms on
HBsAg seroclearance was more pronounced in younger patients , with the OR for rs3077 minor allele A and haplotype block GAT being 0.560 and 2.68 , respectively , among patients aged & lt;50 years ( P = .027 and P = .047 , respectively ) . -
pubmed
IL28B haplotype block CG ( rs12979860 / rs8099917 ) was associated with
HBsAg seroclearance ( OR , 10.5 , P = .026 ) . -
pubmed
CONCLUSIONS : Specific SNPs in
HLA - DP and
IL28B locus , through individual and haplotype analysis , were associated with a higher chance of
HBsAg seroclearance in
CHB infection . -
pubmed
METHODS AND RESULTS : We conducted metabolite analyses , qRT - PCR of the
glycerol reductive pathway [
glycerol dehydratase (
DhaB ) and
1,3 - PDO dehydrogenase (
DhaT ) ] and
DhaT activity assays at different pH , temperature and initial
glycerol concentrations . -
pubmed
The decreased
DhaT activity eventually caused the shutdown of
1,3 - PDO production . -
pubmed
In this review , we highlighted the recent progress in multinuclear
platinum complexes and
ruthenium complexes as anticancer agents , and their novel DNA binding properties such as
phosphate clamps , long range DNA cross links , bisintercalation , interduplex cross links and DNA - protein cross - links were summarized to shed light on the rational design of polynuclear complexes as anticancer agents . -
pubmed
Here we investigated the effect of
tanshinone II A on
Tat - induced HIV-1 transactivation and the redox signaling pathway involved in it . -
pubmed
Tanshinone II A reversed
Tat - induced inhibition of
SIRT1 activity but not
SIRT1 protein expression . -
pubmed
Tanshinone II A - evoked
Nampt expression was mediated by
AMPK signaling pathway . -
pubmed
PURPOSE : To evaluate the topical instillation of
K-115 , a selective
Rho - associated coiled coil - containing protein kinase (
ROCK ) inhibitor , on intraocular pressure ( IOP ) , ocular distribution , and aqueous humor dynamics in experimental animals . -
pubmed
METHODS :
Kinase inhibition by
K-115 was measured by biochemical assay . -
pubmed
RESULTS : Biochemical assay showed that
K-115 had selective and potent inhibitory effects on
ROCKs . -
pubmed
CONCLUSIONS : These results indicated that
K-115 ophthalmic solution , a selective and potent
ROCK inhibitor , is a novel and potent antiglaucoma agent . -
pubmed
Isopentenyl diphosphate isomerase (
IDI ) is a key enzyme in the
isoprenoid biosynthetic pathway and is required for all organisms that synthesize
isoprenoid metabolites from
mevalonate . -
pubmed
Steady - state kinetic studies of the
enzyme indicated that
FMNH2 ( KM = 0.3 muM ) bound before
isopentenyl diphosphate ( KM = 40 muM ) in an ordered binding mechanism . -
pubmed
At 30 days ,
TRA compared to
TFA was associated with a significant reduction of cardiovascular mortality ( 5.2 % vs 10.5 % ; P<.001 ) , significant MACE reduction ( 7.3 % vs 12.5 % ; P<.001 ) , fewer access - site complications ( 0.9 % vs 8.2 % ; P<.001 ) , and lower TIMI major
bleeding ( 1.1 % vs 4.3 % ; P<.001 ) . -
pubmed
We report here a facile approach to fabricating
poly ( l - lactic acid )-grafted
hydroxyapatite ( g - HAp)/
poly ( lactide - co - glycolide ) (
PLGA ) nanocomposite (
NC ) porous scaffolds by solvent evaporation of Pickering high internal phase emulsion ( HIPE ) templates , where g - HAp nanoparticles act as particulate stabilizers . -
pubmed
Enzymatic Studies of
Isoflavonoids as Selective and Potent Inhibitors of
Human Leukocyte 5 - Lipo - Oxygenase . -
pubmed
Continuing our search to find more potent and selective
5 - LOX inhibitors , we present now the enzymatic evaluation of seventeen
isoflavones ( IR ) and nine
isoflavans (
HIR ) , and their in vitro and in cellulo potency against
human leukocyte 5 - LOX . -
pubmed
Of the 26 compounds tested , 10
isoflavones and 9
isoflavans possessed micromolar potency , but only three were selective against
5 - LOX (
IR-2 ,
HIR-303 , and
HIR-309 ) , with IC50 values at least 10 times lower than those of
12 - LOX , 15 -
LOX-1 , and
15 - LOX-2 . -
pubmed
Of these three ,
IR-2 (
6,7 - dihydroxy-4 - methoxy - isoflavone , known as
texasin ) was the most selective
5 - LOX inhibitor , with over 80 - fold potency difference compared with other
isozymes ; Steered Molecular Dynamics ( SMD ) studies supported these findings . -
pubmed
The presence of the
catechol group on ring A (
6,7 - dihydroxy versus
7,8 - dihydroxy ) correlated with their biological activity , but the reduction of ring C , converting the
isoflavones to
isoflavans , and the substituent positions on ring B did not affect their potency against
5 - LOX . -
pubmed
Cognitive functioning , leisure - time physical activity ( LTPA ) , serum trace elements -
Fe ,
Cu ,
Zn ,
Zn /
Cu , and GADAs were assessed using LOTCA battery , pre - validated physical activity (
PA ) questionnaire , atomic absorption , and immunoassay techniques , respectively . -
pubmed
LOTCA - cognitive scores correlated positively with sex , HbA(1c ) ,
Fe ,
Cu ,
Zn , and
Zn /
Cu ratio , and negatively with age ,
PA ,
body mass index , and anti - GADAs . -
pubmed
In this study , we used affinity purification coupled with liquid chromatography and mass spectrometric analysis of the digested protein for consistent and simultaneous detection of the full
amino acid sequence of a therapeutic
IgG4 monoclonal antibody , MAB1 . -
pubmed
Macronutrient meal ingestion significantly reduced
FMD , an effect that was moderated by
body mass index , sex and two - way interactions between disease status and both baseline
FMD and baseline blood
glucose levels . -
pubmed
Using
protease cleavage - predicting software and site - directed mutagenesis , we identified that
calpain-1 cleaves
hERG at position
Gly-603 in the
S5 - pore linker of
hERG . -
pubmed
The aim of this study was to evaluate the consumption , performance , feed efficiency ,
glucose concentration , and the cost per kilogram of
weight gain of crossbred Holstein - Gyr heifers ( n = 33 ) subjected to one of the three waste milk step - down strategies : 6L-30d/4L-60d/2L-90d (
T1 ) , 6L-45d/4L-60d/2L-90d (
T2 ) , and 8L-30d/6L-60d/3L-90d ( T3 ) . -
pubmed
Insufficient improvement on oral antihyperglycemic therapy and poor feasibility of daily subcutaneous
insulin therapy led to an investigation into an alternative therapy with extended - release
exenatide , a
glucagon - like peptide-1 (
GLP-1 ) mimetic , at a dosage of 0.13 mg / kg subcutaneously once per month . -
pubmed
This defect is in part due to increased apoptosis of
HSPCs with reduced
Erg dosage , a phenotype that becomes more drastic during
5 - FU - induced stress hematopoiesis . -
pubmed
, psychoactive drugs such as
LSD and
psilocybin that exert their effects primarily through agonist activity at
serotonin 2A ( 5HT2A ) receptors . -
pubmed
Cardiopulmonary bypass (
CPB ) surgery , despite
heparin administration , elicits activation of coagulation system resulting in
coagulopathy . -
pubmed
Intracellular
Calcium Mobilization Is Required for
Sonic Hedgehog Signaling . -
pubmed
Reduced expression or modified activity of
ryanodine receptor (
RyR ) intracellular
calcium release channels shifted the allocation of
Shh - dependent cell fates in the somitic muscle and neural tube . -
pubmed
Mosaic analysis revealed that
RyR - mediated
calcium mobilization is required specifically in
Shh ligand - receiving cells . -
pubmed
As modulation of
RyR activity modifies tissue patterning , we hypothesize that alterations in intracellular
calcium mobilization contribute to both
birth defects and evolutionary modifications of morphology . -
pubmed
We found a positive correlation between
sucrose preference and
osteoprotegerin /
receptor activator of nuclear factor kappaB ligand ratio . -
pubmed
The phosphorylation of
MAP2c ,
stathmin , and
JNK1 in the male pup hippocampus was significantly decreased in
DEHP treatment groups compared to controls . -
pubmed
Transmission electron microscopy (
TEM ) analysis demonstrated that the
ZIF-8 nanocrystals were gradually etched out in the aqueous media within 48 h , resulting in hollow nanocapsules . -
pubmed
Study data and outcome measures ( fasting blood
glucose ,
glycated haemoglobin and serum
insulin ) were extracted from included studies . -
pubmed
Vitamin D supplementation was associated with a decrease in fasting blood
glucose by a mean of 0.46 mmol / L ( -0.68 , -0.25 ) ( p & lt ; 0.001 ) ,
glycated haemoglobin by a mean of 0.37 % ( -0.65 , -0.08 ) ( p & lt ; 0.01 ) and serum
insulin concentration by mean of 4.10 microIU / mL ( -5.50 , -2.71 ) ( p & lt ; 0.001 ) compared to controls . -
pubmed
This study aimed to analyze the effect of natural antioxidants , 6.5 %
proanthocyanidin (
PA ) and 25 % bamboo salt ( BS ) on the reversal of
NaOCl - induced reduced bond strength of an
epoxy resin sealer to dentin . -
pubmed
Both 6.5 % PA and 25 % BS were capable of reversing the compromised
PBS of
AH Plus to
NaOCl - treated dentin . -
pubmed
Conclusion : Final irrigation with antioxidants such as
PA and
BS eliminates the risk of reduced bond strength of AH Plus to root canal walls , which ensues following the use of
NaOCl as an irrigant . -
pubmed
In contrast , prior studies reported that plasmalemmal
NMDA GluN1 increased in
ERbeta - expressing PVN dendrites in males and early , but not late stage AOF females . -
pubmed
Its unique catalytic mechanism involving the two - electron reduction of
quinone - based compounds has made it a useful target to exploit in the design of
hNQO1 fluorescent chemosensors and
hNQO1 - activatable - prodrugs . -
pubmed
Native
phenalenone is liberated in the presence of
hNQO1 resulting in the production of cytotoxic singlet
oxygen upon irradiation . -
pubmed
Plant
K + uptake typically consists low - affinity mechanisms mediated by
Shaker K + channels (
AKT /
KAT /
KC ) and high - affinity mechanisms regulated by
HAK / KUP / KT transporters , which are extensively studied . -
pubmed
HvAKT2 and
HvHAK1 are both localized to the plasma membrane and have high selectivity to K+ and
Rb + over other tested cations . -
pubmed
Overexpression of
HvAKT2 and
HvHAK1 enhanced
K + uptake and
H + homeostasis leading to drought tolerance in these transgenic lines . -
pubmed
Moreover ,
HvAKT2- and
HvHAK1 - overexpressing lines showed distinct response of
K + ,
H + , and
Ca2 + fluxes across plasma membrane and production of
nitric oxide and
hydrogen peroxide in leaves as compared to the wild type and silenced lines . -
pubmed
Upon tissue disruption ,
glucosinolates come into contact with
myrosinase , which initiates their breakdown to biologically active compounds . -
pubmed
Among these , the formation of
epithionitriles is triggered by the presence of
epithiospecifier protein (
ESP ) and a terminal double bond in the
glucosinolate side chain . -
pubmed
Biochemical characterization of heterologous expressed
BoESP isoforms revealed different substrate specificities towards seven
glucosinolates : all isoforms showed epithiospecifier activity on
alkenyl glucosinolates , but not on
non - alkenyl glucosinolates . -
pubmed
oleracea
ESPs in
glucosinolate breakdown . -
pubmed
In conclusion , the most important bioactive components -
anthraquinone ,
kaempferol , and
vanillic acid - can alleviate
depression symptoms by regulating
MAOA ,
MAOB , and
ESR1 . -
pubmed
A novel highly frequent single -
nucleotide polymorphism site of
cadherin 23 in
clear cell renal cell carcinoma with sarcomatoid differentiation based on whole exome sequencing . -
pubmed
A highly frequent mutation of
CDH23 ( rs3802711 ) was observed in
CCRCCS that resulted in an alteration in the highly conserved
calcium - binding site in the three - dimensional ( 3D ) structure mediating the functions of
cadherins . -
pubmed
Inhibition of
semaphorin 4D enhances chemosensitivity by increasing
5 - fluorouracile - induced apoptosis in
colorectal cancer cells . -
pubmed
In this study , we explored the relationship between silencing
SEMA4D expression and
5 - fluorouracil (
5 - FU ) response in the
colorectal cancer cell line . -
pubmed
Our data demonstrate that
SEMA4D silencing results in strikingly elevated apoptosis in response to
5 - FU treatment and leads to down - regulation of
Bcl-2 and overexpression of
Bax ,
P53 , and
caspase-3 in protein levels . -
pubmed
However , mRNA expression of
E - cadherin as an epithelial marker was significantly increased in
5 - FU treatment combined with siRNA
SEMA4D. -
pubmed
We demonstrate that
infection of wt macrophages results in activation of
Asm , which increases reactive
oxygen species ( ROS ) via stimulation of
nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase . -
pubmed
RIFM fragrance ingredient safety assessment ,
2 - nonanone ,
CAS Registry Number 821 - 55 - 6 . -
pubmed
TSLP concentration was measured by ELISA after
acetone precipitation of the samples . -
pubmed
ACE2 is part of the counter - regulatory
renin -
angiotensin -
aldosterone system and is also expressed in the lower respiratory tract along the alveolar epithelium . -
pubmed
WSD exposure also altered ovarian cycles , delaying the time to the
E2 surge , decreasing
progesterone and anti - Mullerian hormone levels and increasing the number of antral follicles present by ultrasound . -
pubmed
H2O2 was supplied exogenously by
glucose oxidase , and
glucose was produced at a level which was itself noninhibitory . -
pubmed
Two phases were distinguished : the first phase was dependent on the oxidation of
SCN (- ) by
lactoperoxidase and
H2O2 , which was reversed by reducing agent , and the second phase was dependent on the presence of accumulated
H2O2 , which was reversed by
catalase . -
pubmed
Pre - operative replacement of haemodialysis by peritoneal dialysis is advisable to facilitate the treatment of
cerebral oedema , maintain the
electrolyte and water balance and ensure adequate
post - operative enteral and parenteral feeding . -
pubmed
Specific inhibitors of the
endoplasmic - reticulum Ca2 + pump will deplete intracellular stores and are therefore useful to study the role of store depletion on plasma - membrane
Ca2 + permeability . -
pubmed
There was no significant change in the RNA levels of several other starch biosynthetic enzymes , but there was a great increase in the RNA level of the major
sucrose synthesizing enzyme
sucrose phosphate synthase . -
pubmed
For each stage of the estrous cycle , the enzymatic activities were quantified by means of the
3H2O - release assay in the case of
P450AROM and thin - layer chromatography in the case of
5 alpha - reductase . -
pubmed
The proportion of
L - tryptophan metabolized via the latter flux increased over 10 - fold ( 75 % of total
tryptophan metabolized ) as the concentration of
L - tryptophan was raised from 5 x 10(-5 ) to 5 x 10(-4 ) M.
L - Tryptophan metabolized via the
kynureninase flux was less than 5 % of total
tryptophan metabolized . -
pubmed
2 - 3H liberation increased dose - dependently at
tryptophan concentration higher than 10(-5)M , while the
kynureninase flux reached its plateau at 10(-5)M. Formation of
tryptophol and
indole-3 - acetic acid via
indole-3 - pyruvic acid and
indole-3 - acetaldehyde with
indole aldehyde as a by - product was confirmed using exogenous
tryptophan metabolites with
indole rings . -
pubmed
The following findings characterized the poly(ADPR ) reaction : ( 1 ) pretreatment of nuclear extracts with snake venom
phosphodiesterase which removes
poly ( ADPR ) chains strongly reduced the material which was retained by
phenylboronate ; ( 2 ) the
ADPR transferase inhibitor
benzamide ( 100 microM ) suppressed
AO - induced poly(ADP - ribosylation ) ; ( 3 ) poly(ADP - ribosylation ) reduced the electrophoretic mobility of the modified
histones . -
pubmed
The extent of substitution 30 min after exposure to AO generated by 50 micrograms / mL
xanthine and 5 micrograms / mL
xanthine oxidase was 0.8 % for
A24 greater than 0.3 % for
H4 greater than 0.1 % for H3.1 = 0.1 % for
H3.2 = 0.1 % for
H2B.2 greater than 0.09 % for
H2A. -
pubmed
Within 60 min ,
poly ( ADPR ) substitution had decreased to control levels for H3 and
H4 and below control levels for
H2A and
H2B.(ABSTRACT TRUNCATED AT 250 WORDS ) -
pubmed
The dyes
ethidium bromide ( EB ) ,
propidium iodide (
PI ) , and
acridine orange ( AO ) were used in this study . -
pubmed
Hormonal concentrations (
thyrotropin [
TSH ] ,
prolactin [
PRL ] ,
thyroxin ,
triiodothyronine , and reverse
triiodothyronine ) before and during a
TRH test were assessed in the 2 groups before ( D0 ) and after ( D7 ) each treatment . -
pubmed
The
TSH ( peak values and Kr ) and
PRL ( peak values and AUC ) responses to
TRH were significantly decreased in the
nifedipine group compared to the placebo group . -
pubmed
No relationship existed between urinary
NAG and serum
creatinine ,
beta-2 - microglobulin , and degree of metabolic control ( HbA7 ) . -
pubmed
p24 is a thyroid protein ( Mr 24,000 ) identified by two - dimensional gel electrophoresis on the basis that its synthesis and phosphorylation are up - regulated by
thyrotropin and
cyclic AMP agonists . -
pubmed
The encoded polypeptide ( 189 residues ) displays a putative target - site for phosphorylation by
cyclic AMP - dependent protein kinase and belongs to the superfamily of proteins binding
Ca2 + through ' EF hand ' domains . -
pubmed
The ability of
p24 to bind
Ca2 + has been directly confirmed on Western blots . -
pubmed
The ubiquitous nature of
p24 , together with its regulatory and sequence characteristics suggest that it constitutes an important target common to the
cyclic AMP and
Ca2 + -
phosphatidylinositol cascades . -
pubmed
The
metR gene was subcloned from pRSE562 , and this plasmid , pMRIII , relieved the
methionine auxotrophy of a
metR mutant after transformation . -
pubmed
The in vitro expression of both genes was inhibited by the
MetJ protein and
S - adenosylmethionine in the presence or absence of
MetR protein . -
pubmed
The concentration of
protein kinase C was also determined by using
[ 3H ] phorbol-12,13 - dibutyrate (
PDBu ) . -
pubmed
We found that
calcium influx was unaltered under basal conditions but that the ability of
norepinephrine ( NE ) to augment influx was significantly depressed ( P less than .05 ; [ control vs. septic , 572 + /- 54 [
SE ] vs. 428 + /- 30 mumol
Ca2+/kg dry wt . -
pubmed
In conclusion , we found that during
sepsis alpha 1 - adrenergic receptor activation of both
calcium influx and efflux by NE is decreased ; these alterations could be related to the depressed aortic contractility observed in
sepsis . -
pubmed
The initial GM PC20
histamine for the group was 22.9 mg / ml , which did not change significantly with two further
histamine challenges or when the airways were refractory to
AMP , suggesting that loss of
AMP effect was not related to loss of contractility of airway smooth muscle or down regulation of
H1 - histamine receptors.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
The
corticotropin - releasing factor is the predominant component of a complex control system of adrenal cortex secretion , which also includes
catecholamines and the
antidiuretic hormone . -
pubmed
Treatment of cells with
dibutyryl adenosine 3 ' : 5 '- cyclic monophosphate ( 1 mmol / l ) or
forskolin ( 5 mumol / l ) increased the levels of cytosolic
GH mRNA by between 1.6- and 4.7 - fold . -
pubmed
These data demonstrate that
cyclic adenosine nucleotides may mediate the
GRF induction of
GH gene transcription . -
pubmed
Cerebrospinal fluid (
CSF )
glycine levels did not change during treatment , suggesting inadequate penetration into the central nervous system of
glycine at the dosage used . -
pubmed
Before , during and after this treatment , serum levels of
aMT ,
PRL ,
LH ,
FSH and
testosterone were examined . -
pubmed
Although
aMT increased at least 1,500 - fold over basal levels , only
PRL was significantly and consistently elevated after
aMT treatment , whereas serum levels of the other hormones were not altered . -
pubmed
Serum
PRL levels were significantly higher after
aMT than after placebo ;
GH showed a slight but not significant trend towards elevation after
aMT , whereas other hormones were not altered . -
pubmed
An acute pharmacological dose of
aMT causes isolated elevation of serum
PRL levels and may slightly increase
GH . -
pubmed
Effects of
bromocriptine on
prolactin cellular
hypertrophy , proliferation and secretory activity in
diethylstilbestrol - induced
pituitary tumors . -
pubmed
BC treatment of
tumors reversed the effects of
DES on pituitary weight ,
PRL content and in vitro
PRL release . -
pubmed
The results establish that
PRL cell
hypertrophy , as well as
hyperplasia , results from
DES treatment . -
pubmed
Bromocriptine treatment reverses this
hypertrophy concomitant with inhibiting
PRL synthesis and release . -
pubmed
The distribution of
Copper -
Zinc containing and
Manganese - containing
superoxide dismutase in the liver , kidney , interscapular brown adipose tissue (
IBAT ) and brain of the ground squirrel , as well as the effect of hibernation , was studied . -
pubmed
TEER was inversely correlated with
22Na transport ( r2 = 0.93 , P = less than 0.001 ) but not with 125I -
LDL transport . -
pubmed
Removal of
beta - VLDL from the culture medium resulted in the return of cellular
cholesterol content and
LDL transport to control values . -
pubmed
The
permanganate method , the
immunoperoxidase method , and a newly developed autoclave method were used to distinguish different types of amyloid fibril proteins in
formalin - fixed , paraffin - embedded tissue sections . -
pubmed
On the other hand ,
prealbumin type systemic
amyloidosis and
senile plaques of
SDAT were resistant to both
permanganate oxidation and prolonged autoclaving . -
pubmed
[ Studies on the structure of
bovine serum kininogen , using
cyanogen bromide and
carboxypeptidase B ] . -
pubmed
Maximal
oxygen consumption ,
pulmonary function , body composition , and anthropometry of adolescent female athletes . -
pubmed
In vivo staining of
cytoskeletal actin by autointernalization of nontoxic concentrations of
nitrobenzoxadiazole -
phallacidin . -
pubmed
Although cells tend to exclude the fluorescent toxin , reducing the internal concentration by approximately 1,000 times , some of it enters the cells , probably by pinocytosis , and stains
actin structures at low intracellular
NBD - Ph concentrations ( approximately 5 - 15 nM ) , where
cell toxicity was negligible or at least not detectable by phase - contrast microscopy . -
pubmed
Derivatives of
cytochrome c having
S - methyl cysteine and
ethionine substituted for
methionine residue 80 have been synthesized in order to study the effects of structural perturbations near the
heme on the biological function of
cytochrome c. -
pubmed
The
etionine derivative has 96 % as much activity as native
cytochrome c in the
succinate oxidase system , whereas the
S - methyl cysteine analog is totally inactive . -
pubmed
Epinephrine acutely mediates skeletal muscle
insulin resistance . -
pubmed
In this study we investigated the influence of
epinephrine on
insulin - mediated
glucose uptake by peripheral tissue . -
pubmed
Epinephrine - induced skeletal muscle
insulin resistance may play a major role in
insulin - resistant states and may contribute to accelerated protein catabolism seen following
injury . -
pubmed
The polymorphisms of
alcohol and
aldehyde dehydrogenase and their significance for
acetaldehyde toxicity . -
pubmed
The feasibility of quantifying end point histochemistry and relating oxidative staining density ( reduced
nicotinomide adenine dinucleotide diaphorase :
NADH - D ) to whole body maximal
oxygen consumption ( VO2 max ) was also investigated , while muscle fiber types , classified according to
alkaline APTase stains , were studied and related to muscle oxidative capacity (
succinate dehydrogenase :
SDH ) , VO2 max and " in vivo " torque - velocity properties . -
pubmed
While results confirm earlier reports on skeletal muscle properties and performance it was concluded that end point histochemistry could be reliably quantified and that an " oxidative " stain such as
NADH - D correlates extremely well with VO2 max ( r = 0.86 , p less than 0.001 ) whereas correlations between % slow twitch fibres (
Alkaline ATPase stain ) and VO2 max were lower ( r = 0.44 , p less than 0.05 ) . -
pubmed
Furthermore , in type
IV allergic model , CM - ext had the inhibitory effects on induction phase and effector phase in
picryl chloride - induced
contact dermatitis ( PC -
CD ) . -
pubmed
It solved the analytical problem that artefact
TBC and
MTBC are formed during analysis . -
pubmed
The calibration graphs showed a good linearity in the range 0.1 - 50.0 ng ml-1 of urine samples spiked with standard
TBC and
MTBC . -
pubmed
Protein purity was assessed by
silver - stained native and
sodium dodecyl sulfate polyacrylamide gel electrophoresis (
SDS PAGE ) . -
pubmed
The curve relating Vt to conditioning potential had a negative slope ( which was insensitive to addition of 1 mM
cobalt or replacement of
calcium with 20 mM
CaEGTA ) between -60 and -35 mV and a steep positive slope with further depolarization Unexpectedly , fibers became inactivatable with less conditioning depolarization in soleus than in
EDL when Vt was measured with 50 msec test
pulses . -
pubmed
The aim of this study was to know about the plasmatic variations of
insulin and its potential origin in the
obesity with or without abnormal tolerance to
glucose . -
pubmed
Early transient incapacity and
cerebral radiation syndrome were shown to result from the de - energizing of the brain due to
NAD and
ATP neuron pools depletion since DNA breaks stimulate the repair enzyme ,
ADP - ribosyltransferase , that consumes
NAD as a substrate . -
pubmed
In contrast to thermolabile
prenyltransferases , which have four to six
cysteine residues , the thermostable
farnesyl diphosphate synthase carries only two
cysteine residues . -
pubmed
This
enzyme is also unique in that some of the
amino acids that are fully conserved in equivalents from other sources are replaced by functionally different
amino acids . -
pubmed
GAL4 -
p53 chimeras constructed using naturally occurring
p53 mutations at either codon 141 (
Tyr-141 ) or 175 (
His-175 ) of
p53 had little ability to activate the reporter gene ; in contrast , mutations at either codon 248 ( Trp-248 ) or 273 (
His-273 ) produced greater transcriptional activities than did wild - type
p53 . -
pubmed
Surprisingly , coexpression of either
p53 ( Trp-248 ) or ( His-273 ) with the GALA -
p53 ( wild - type ,
His-273 ,
Trp-248 ,
His-175 ,
Tyr-141 ) effectors conferred an increase in transcriptional activation as compared with the
effector alone . -
pubmed
p53 ( Tyr-141 /
His-273 ) behaved as a dominant negative mutant and inhibited the ability of the combination of
p53 ( His-273 ) and
GAL4 -
p53 ( C - terminus ) to stimulate the reporter gene . -
pubmed
Double immunoprecipitation by sequentially using
GAL4 and
p53 antibodies showed that
p53 ( His-273 ) and (
Tyr-141 /
His-273 ) , but not
p53 ( Tyr-141 ) , can efficiently oligomerize in vivo to the C - terminal region of
p53 . -
pubmed
Transcriptional activating function of
p53 may be modulated by oligomerization ; some mutations , such as
His-273 and
Trp-248 , participate in these functions. -
pubmed
Proof of this reaction is found in addition of
superoxide dismutase (
SOD ) to the system , which totally eliminates the contribution of the
Trp . -
pubmed
The entrainment with
melatonin was restricted to a narrow window for
melatonin to be applied , since injections at
CT8 did not result in entrainment . -
pubmed
Lack of association between polymorphisms in the
5 - HT2A receptor gene and the antipsychotic response to
clozapine . -
pubmed
OBJECTIVE : The authors ' goal was to determine whether either of two
5 - HT2A receptor polymorphisms , 102 - T / C and 452 -
His /
Tyr , are associated with
clozapine response . -
pubmed
Our study revealed that surgical interventions in
lung adenocarcinomas resulted in a marked ( P & lt ; or = 0.01 ) and long - lasting ( 5 days ) release of
neopterin and
sIL-2 - R postoperatively . -
pubmed
Regulation of
tyrosine hydroxylase gene expression during
hypoxia : role of
Ca2 + and
PKC . -
pubmed
Gene expression for
tyrosine hydroxylase (
TH ) , the rate - limiting enzyme in
catecholamine biosynthesis , is regulated by reductions in
oxygen tension (
hypoxia ) . -
pubmed
Based on the evidence that the transcription factors that bind to the
TH promoter during
hypoxia can also be induced by elevations in cytosolic
Ca2 + , the role of
Ca2 + in the hypoxic regulation of the
TH gene was explored . -
pubmed
The addition of the
L - type calcium channel blockers
nifedipine or
verapamil caused partial inhibition of the
hypoxia - induced increase in
TH mRNA . -
pubmed
Importantly , chelation of extracellular
Ca2 + completely inhibited the increase in
TH mRNA by
hypoxia . -
pubmed
Addition of
chelerythrine chloride (
CHL ) , a
protein kinase C inhibitor , to the media before exposure to
hypoxia also resulted in an inhibition of
TH induction by
hypoxia . -
pubmed
These results suggest that
hypoxia regulates
TH gene expression by a mechanism that is dependent on influx of
calcium from the extracellular stores , partially but not exclusively through the
L - type calcium channels . -
pubmed
RBCK2 , a cDNA fragment related to
RBCK1 was obtained , that lacks the 161 - bp sequence of
RBCK1 and encodes 260
amino acid residues . -
pubmed
The 240 -
amino acid sequence in the NH2 - terminal of
RBCK2 is identical with
RBCK1 and contains two coiled -
coil regions but no other structural motifs , whereas the 20 -
amino acid sequence in the COOH - terminal is distinct from
RBCK1 . -
pubmed
Biexciton formation in
GaAs /
AlAs type -
II superlattices under extremely low excitation powers . -
pubmed
PAI-1 antigen levels measured in conditioned media after a 19 - hour incubation period of adipose tissue explants were significantly correlated with plasma
PAI-1 antigen levels ( r=0.54 , P=0.004 ) and with systemic lipid parameters such as
triglycerides and
high density lipoprotein cholesterol ( r=0 . -
pubmed
Phage display was used to identify peptides that interact specifically with either
estradiol- or
tamoxifen - activated
estrogen receptor alpha . -
pubmed
When these peptides were coexpressed with
estrogen receptor alpha in cells , they functioned as ligand - specific antagonists , indicating that
estradiol - agonist and
tamoxifen - partial agonist activities do not occur by the same mechanism . -
pubmed
The ability to regulate
estrogen receptor alpha transcriptional activity by targeting sites outside of the ligand - binding pocket has implications for the development of
estrogen receptor alpha antagonists for the treatment of
tamoxifen - refractory
breast cancers . -
pubmed
Virologic and
CD4 + cell responses to new
nucleoside regimens : switching to
stavudine or adding
lamivudine after prolonged
zidovudine treatment of
human immunodeficiency virus infection . -
pubmed
Both regimens significantly increased
CD4 + cell numbers , the means of weeks 40 and 48 rose to 49 and 36
CD4 + cells per cubic millimeter among
zidovudine plus
lamivudine and
stavudine recipients , respectively . -
pubmed
It has been suggested that the
cystic fibrosis transmembrane conductance regulator (
CFTR )
chloride channel may utilize a novel gating mechanism in which open and closed states are not in thermodynamic equilibrium . -
pubmed
We demonstrate that
CFTR channel gating occurs in the absence of
ATP hydrolysis and hence does not depend on an input of free energy from this source . -
pubmed
These observations demonstrate that phosphorylated
CFTR behaves as a conventional ligand - gated channel employing cytoplasmic
ATP as a readily available cytoplasmic ligand ; under physiological conditions ligand hydrolysis provides efficient reversibility of channel opening . -
pubmed
Allopregnanolone is a well - established allosteric modulator of the
GABAA receptor but its physiological roles within the nervous system remain unclear . -
pubmed
Protein kinase C and
guanosine triphosphate combine to potentiate
calcium - dependent membrane fusion driven by
annexin 7 . -
pubmed
Exocytotic secretion is promoted by the concerted action of
calcium ,
guanine nucleotide , and
protein kinase C. -
pubmed
We now show that the
calcium - dependent membrane fusion activity of
annexin 7 in vitro is further potentiated by the combined addition of
guanine nucleotide and
protein kinase C. -
pubmed
Guanosine triphosphate (
GTP ) and its non - hydrolyzable analogues optimally enhance the phosphorylation of
annexin 7 by
protein kinase C in vitro . -
pubmed
Reciprocally , phosphorylation by
protein kinase C significantly potentiates the binding and hydrolysis of
GTP by
annexin 7 . -
pubmed
In vivo , the ratio of bound
GDP /
GTP and phosphorylation of
annexin 7 change in direct proportion to the extent of
catecholamine release from chromaffin cells in response to stimulation by
carbachol , or to inhibition by various
protein kinase C inhibitors . -
pubmed
These results thus lead us to hypothesize that
annexin 7 may serve as a common site of action for
calcium ,
guanine nucleotide , and
protein kinase C in the exocytotic membrane fusion process in chromaffin cells . -
pubmed
The State Scale of Dissociation (
SSD ) was administered with other psychiatric scales (
DES , BDI , BAI , SCI - PANSS ) to 130 participants with DSM -
IV major
depressive disorder schizophrenia ,
alcohol withdrawal ,
dissociative disorders and controls . -
pubmed
All three forms of structural remodeling of the hippocampus are mediated by hormones working in concert with excitatory
amino acids (
EAA ) and
N - methyl - D - aspartate ( NMDA ) receptors . -
pubmed
EAA and
NMDA receptors are also involved in neuronal
death that is caused in pyramidal neurons by
seizures and by
ischemia and prolonged psychosocial stress . -
pubmed
FK506 is an immunophilin - binding ligand that inhibits
calcineurin and decreases
nitric oxide (
NO ) production in the nervous tissues . -
pubmed
tolerance and dependence and compared them with the effects of the non - specific
NO synthase inhibitor ,
NG - nitro - L - arginine methyl ester (
L - NAME ) , and specific inducible
NO synthase inhibitor ,
aminoguanidine . -
pubmed
Decarboxylases involved in
polyamine biosynthesis and their inactivation by
nitric oxide . -
pubmed
Ornithine decarboxylase is a
PLP - requiring
decarboxylase , whereas
S - adenosylmethionine decarboxylase (
AdoMetDC ) contains a covalently bound
pyruvate prosthetic group . -
pubmed
These residues ,
Cys360 in
ornithine decarboxylase (
ODC ) and
Cys82 in
AdoMetDC , react readily with
nitric oxide (
NO ) , which is therefore a potent inactivator of
polyamine synthesis . -
pubmed
oryzae
beta - galactosidase ) in the initial content of
SH groups in the
lactases . -
pubmed
lactis
beta - galactosidase with
thiopropyl - agarose than for the same reduction with
DTT . -
pubmed
oryzae
beta - galactosidase , nearly twice as many micromoles of
SH groups per milligram of
protein were needed with
thiopropylagarose than with
DTT . -
pubmed
Disulfide bonds in
beta - lactoglobulin were not accessible to
thiopropyl - agarose , since this reduction was only possible in the presence of 6 M
urea . -
pubmed
These results proved that highly substituted
thiopropyl - agarose is as good a reducing agent as
DTT , for the reduction of
disulfide bonds in
proteins . -
pubmed
Free
fatty acid (
FFA ) concentrations in cerebrospinal fluid (
CSF ) are recognized as markers of
brain damage in animal studies . -
pubmed
Higher concentrations of total
polyunsaturated fatty acids ( P<0.001 ) and of
arachidonic , myristic and palmitic acids measured individually in
CSF ( P<0.01 ) obtained 1 week after the insult were associated with a worse outcome at the time of hospital discharge using the Glasgow Outcome Scale . -
pubmed
This preliminary investigation suggests that
CSF FFA concentrations may be useful as a predictive marker of outcome following
TBI . -
pubmed
Alkaline phosphatase in leucocytes by an
azo dye method . -
pubmed
Serum and erythrocyte
folate and serum
vitamin B12 was determined by radioassay ;
serum ferritin by enzimoimmunoassay ;
hemoglobin were performed by semi - automated method . -
pubmed
Improved HPLC method for
carbohydrate - deficient
transferrin in serum . -
pubmed
BACKGROUND : There is need for a reference method for
transferrin glycoforms in serum to which routine immunologic methods for the
alcohol marker
carbohydrate - deficient
transferrin (
CDT ) can be traceable . -
pubmed
METHODS :
Transferrin was
iron - saturated by mixing the serum with
ferric nitrilotriacetic acid , and lipoproteins were precipitated with
dextran sulfate and
calcium chloride . -
pubmed
Quantification relied on selective absorbance of the
iron -
transferrin complex at 470 nm . -
pubmed
Serum and the pretreated samples were stable for at least 2 days at 22 or 4 degrees C. Sera from 132 healthy controls contained [ mean ( SD ) ] 1.16 ( 0.25)%
disialotransferrin , 4.77 ( 1.36)%
trisialotransferrin , 80.18 ( 2.01)% tetrasialotransferrin , and 13.88 ( 1.69)%
pentasialo - + hexasialotransferrin . -
pubmed
BDCM treatment reduced DC and
GAL activities and elevated
NR and
AR activity . -
pubmed
GAL ,
AR , and
NR activities were significantly different after treatment with
bromate ,
chloroform ,
BDCM , and MX , but not the mixture . -
pubmed
They have shown that high concentrations of
ethanol lead to
necrosis , while low concentrations induce apoptosis due to activation of
Fas - receptors . -
pubmed
Triggering of apoptosis through
Fas - receptors represents a mechanism of action different from that observed with high concentrations of
ethanol , thus , reducing the complications that follow the inflammatory process due to
necrosis . -
pubmed
Mean
TRPV6 expression was the same , but there was evidence of different responsiveness to
1,25 ( OH )( 2 ) D ( 3 ) . -
pubmed
In the GG genotype group , but not the AG , duodenal
TRPV6 expression increased with
1,25 ( OH )( 2 ) D ( 3 ) . -
pubmed
We postulate that lower levels of expression of
VDR in the GG group produce greater sensitivity to
1,25 ( OH )( 2 ) D ( 3 ) . -
pubmed
We then explored why these cells with
arsenite - induced DNA damage were arrested at mitosis instead of
G2 stage . -
pubmed
Immunoblot analysis confirmed that
arsenite treatment reduced the X - irradiation - induced phosphorylation of both
ataxia - telangiectasia , mutated at
serine 1981 and
Cdc25C at
serine 216 , events which are crucial for G2 checkpoint activation and G2 arrest . -
pubmed
Synthesis , inhibition properties , and theoretical study of the new nanomolar
trehalase inhibitor
1 - thiatrehazolin : towards a structural understanding of
trehazolin inhibition . -
pubmed
1 - Thiatrehazolin is a very potent , slow , tight - binding
trehalase inhibitor . -
pubmed
A structural model for
trehalase inhibition by
trehazolin and its analogues , based on the experimental results and supported by theoretical calculations , is proposed . -
pubmed
Time - resolved fluorescence experiments using
tryptophan excitation have shown that
RET is manifested by a distinct growing in of acceptor fluorescence at a rate characteristic for this process . -
pubmed
The method is based on
methanol - modified
carbon dioxide as the mobile phase at flow rate of 3.0 ml / min with elution through a JASCO Finepak SIL-5 , [
C18 ( 5 - micron , 25 cm x 4.6 mm , i.d . ) -
pubmed
Nifedipine gastrointestinal therapeutic system ( GITS ) is a long - acting formulation of a
calcium channel blocker . -
pubmed
The combination of
vasostatin gene therapy and
cyclophosphamide administration improved therapeutic effects in
melanoma tumours . -
pubmed
These observations led the authors to conclude that the identity of
BIC is dubious , most probably it will represent a subtype of
BPEI . -
pubmed
Current data now suggest that
caveolin-1 plays an essential role in the regulation of liver regeneration and that it is implicated in the regulation of
triglyceride accumulation , which is an essential process that plays a critical role in the regulation of liver regeneration . -
pubmed
Methylation of
estrogen receptor alpha and
mutL homolog 1 in
normal colonic mucosa : association with
folate and
vitamin B-12 status in subjects with and without
colorectal neoplasia . -
pubmed
OBJECTIVE : We tested the hypothesis that biomarkers of
folate and
vitamin B-12 status are associated with
estrogen receptor alpha (
ERalpha ) and
mutL homolog 1 (
MLH1 ) promoter methylation in subjects with and without
neoplasia . -
pubmed
DESIGN : Biopsies of
normal - appearing colorectal mucosa from 156 subjects with and without
colorectal neoplasia (
disease free , n = 76 ;
cancer , n = 28 ;
adenoma , n = 35 ;
hyperplastic polyps , n = 17 ) were obtained at colonoscopy and used to evaluate methylation in 7
CpG sites in the
ERalpha promoter and 13
CpG sites in the
MLH1 promoter . -
pubmed
Methylation indexes were generated to reflect an average methylation value across all
CpG dinucleotides in both
ERalpha and
MLH1 . -
pubmed
The
ERalpha methylation index correlated negatively with serum
vitamin B-12 ( r = -0.239 , P = 0.003 ) and positively with plasma
homocysteine ( r = 0.188 , P = 0.021 ) . -
pubmed
Disease status ( P & lt ; 0.005 ) , age ( P & lt ; 0.001 ) , and serum
vitamin B-12 concentrations ( P = 0.006 ) were independent determinants of
ERalpha promoter methylation . -
pubmed
Serum and red cell
folate concentrations had no influence on
ERalpha promoter methylation . -
pubmed
CONCLUSION : Serum
vitamin B-12 but not
folate status may be associated with
ERalpha promoter methylation in
normal - appearing colorectal mucosa . -
pubmed
Five variables including the Child - Pugh score ,
albumin ,
bilirubin ,
ascites , and HV waveform significantly correlated with the survival in a univariate analysis . -
pubmed
RESULTS : Mutation frequencies for
rifampicin ( MIC & gt;or= 0.2 mg / L ) did not differ significantly between serovars L(2 ) and D ( 5.7 x 10(-7 ) versus
6.3 x 10(-7 ) ) . -
pubmed
Moxifloxacin resistance of all serovar L(2 ) clones depended on single - nucleotide point mutations in the
quinolone resistance - determining region of the
gyrA , whereas no additional mutations were found in the
gyrB ,
parC or
parE genes . -
pubmed
We examined whether the risk of
TGCC is associated with serum levels of 11
organochlorine pesticides , including
p , p '- DDE , and whether the
p , p '- DDE - TGCC association is modified by CAG or
GGN repeat polymorphisms in the
androgen receptor gene . -
pubmed
We observed no clear patterns between
TGCC risk and concentrations of any of the
organochlorines measured , nor did we observe that the risk associated with
p , p '- DDE was modified by
androgen receptor CAG ( & lt;23 versus & gt ; or = 23 repeats ) or
GGN ( & lt;17 versus & gt ; or = 17 repeats ) genotype . -
pubmed
Ferritin formation was the highest in cells treated with SFP compared to those treated with other
iron compounds or chelates . -
pubmed
Adding
ascorbic acid ( AA ) and
cysteine to SFP in a 20:1 molar ratio increased
ferritin formation by 3- and 2 - fold , respectively , whereas adding
citrate had no significant effect on the bioavailability of SFP . -
pubmed
The proposed KDRI includes 14 donor and transplant factors , each found to be independently associated with graft failure or
death : donor age , race , history of
hypertension , history of
diabetes , serum
creatinine , cerebrovascular cause of
death , height , weight , donation after
cardiac death ,
hepatitis C virus status ,
human leukocyte antigen - B and
DR mismatch ,
cold ischemia time , and double or en bloc transplant . -
pubmed
The effects of
beta - elemene on the expression of
BCL-2 ,
caspase-3 ,
DR-4 and
NF - kappaB P65 proteins were analyzed by Western blot . -
pubmed
A variable basic cleft on the top of the dimer interface binds to
phosphatidylinositide and regulates the intracellular localization of
PDCD10 . -
pubmed
Of these genes ,
valB , which encodes a
sugar nucleotidyltransferase , was found through inactivation study to be essential for
validamycin biosynthesis , but its role was unclear . -
pubmed
To characterize the role of
ValB in
validamycin biosynthesis , four
carbasugar phosphate analogues were synthesized and tested as substrate for
ValB. -
pubmed
The results showed that
ValB efficiently catalyzes the conversion of
valienol 1 - phosphate to its
nucleotidyl diphosphate derivatives , whereas other
unsaturated carbasugar phosphates were found to be not the preferred substrate . -
pubmed
ValB requires
Mg ( 2 + ) ,
Mn ( 2 + ) , or
Co ( 2 + ) for its optimal activity and uses the
purine - based
nucleotidyltriphosphates (
ATP and
GTP ) more efficiently than the
pyrimidine - based
NTPs (
CTP ,
dTTP , and
UTP ) as
nucleotidyl donor . -
pubmed
While
LPA exerted no significant effect on the cell migration , the prior treatment of the cells with LPS or
IL-1beta resulted in the appearance of migration activity in response to
LPA . -
pubmed
The LPS induction of the migration response to
LPA was associated with the production of
IL-1beta precursor protein and inhibited by the
IL-1 receptor antagonist . -
pubmed
The
IL-1beta treatment also allowed
LPA to activate
Rac1 . -
pubmed
The
LPA - induced
Rac1 activation and migration were inhibited by
pertussis toxin , a small interfering RNA specific to
LPA ( 1 ) receptors , and
LPA ( 1 ) receptor antagonists , including
Ki16425 . -
pubmed
However , the
IL-1beta treatment had no appreciable effect on LPA(1 ) receptor mRNA expression and
LPA - induced activation of
ERK ,
Akt , and proliferation . -
pubmed
The induction of the migration response to
LPA by
IL-1beta was inhibited by a constitutively active
RhoA. -
pubmed
The
aminolevulinic acid dehydratase activity , hematocrit , and
hemoglobin tended to be higher in group E than S. The
iron content in the flexor hallucis longus muscle was significantly higher in E than S , whereas the content in the liver , spleen , kidney , and heart did not significantly differ between the groups . -
pubmed
We measured serum concentrations of
insulin like growth factor - I (
IGF - I ) in 20
thalassemic males with
short stature ( height
SDS & lt;-2 ) and/or slow growth velocity ( GV & lt;-1
SD ) throughout their childhood and adolescence , compared these data with normal reference data validated in our lab , and evaluated their growth hormone secretion in response to
clonidine and
glucagon stimulation . -
pubmed
The method was evaluated by investigating the effect of roll speed for two pharmaceutical excipients with distinctive material properties : microcrystalline cellulose (
MCC ) and
di - calcium phosphate dihydrate (
DCPD ) . -
pubmed
It was found that the maximum pressure and nip angle for
DCPD , which is a cohesive powder , decrease sharply with increasing roll speed whereas they are essentially independent of roll speed for
MCC , which is an easy flowing powder . -
pubmed
Sialoadhesin is a macrophage adhesion molecule containing 17 extracellular
immunoglobulin - like domains , and is an
I - type lectin which binds to
sialic acid ligands expressed on hematopoietic cells . -
pubmed
In this study , a new treatment strategy was proposed consisting of a combination of
5,7 - dimethoxycoumarin (
DMC ) , an activator of melanogenesis , and
valproic acid (
VPA ) , a well - known drug that is one of the
histone deacetylase inhibitors ( HDACis ) . -
pubmed
VPA and
DMC also induced the synthesis of melanin and the formation of dendrite and
star - shaped cells . -
pubmed
Tyrosinase gene expression and
tyrosinase activity significantly increased in response to
VPA treatment . -
pubmed
CNTs with linear
poly ( ethylene glycol ) amphiphiles trigger the
lectin pathway of the complement through both
L - ficolin and
mannan - binding lectin recognition . -
pubmed
An amphiphile with branched
poly ( ethylene glycol ) architecture also activated the
lectin pathway but only through
L - ficolin recognition . -
pubmed
High temperature / light and freeze - thaw treatments verified direct emissions of the
isoprene oxidation products
MVK and MACR together with the first observations of
3 - methyl furan ( 3 - MF ) and
2 - methyl-3 - buten-2 - ol (
MBO ) as putative novel
isoprene oxidation products . -
pubmed
Mechanical wounding also stimulated emissions of
MVK and
MACR . -
pubmed
Three viruses ( 0.9 % ) were found to be
oseltamivir resistant due to an H275Y mutation in the
neuraminidase gene . -
pubmed
In addition ,
GRb1 also increased the activity of
lactate dehydrogenase and
superoxide dismutase in POFS . -
pubmed
Palonosetron hydrochloride is a longer - acting selective
5 - HT3 receptor antagonist that has been approved for the prevention of chemotherapy - induced
nausea and
vomiting and is being evaluated for the prevention of
postoperative nausea and vomiting . -
pubmed
These
hydride transfers are mediated by
B ( C6F5 ) 3 via two
SN2 processes without involving 1 . -
pubmed
Comparison of the deduced
amino acid sequences of
NptA among
strains resulted in
strain grouping similar to that based on transcript abundance . -
pubmed
Contribution of hot
spring bacterial consortium in
cadmium and lead bioremediation through quadratic programming model . -
pubmed
In vitro ,
IQGAP1 mRNA and protein expression were observed to decline under HG media at 48 h. But phosphorylation of
ERK1 / 2 was activated under HG media at 24 h and 48 h. However , HG - induced downregulation of
IQGAP1 protein was attenuated by specific
ERK1 / 2 activation inhibitor
PD98059 . -
pubmed
The controlled release of low - dose ( 1 mu g and 5 mu g )
G - CSF significantly enhanced new bone formation when combined with a
beta - TCP disc . -
pubmed
Moreover , administration of 5 mu g
G - CSF using a controlled release system significantly promoted the biodegradable properties of
beta - TCP . -
pubmed
In conclusion , the controlled release of 5 mu g
G - CSF significantly enhanced the osteoconductive and biodegradable properties of
beta - TCP . -
pubmed
The combination of
G - CSF slow - release and
beta - TCP is a novel and promising approach for treating pediatric
craniofacial bone defects . -
pubmed
During brain development , polysialylated (
polySia ) neural cell adhesion molecules (
polySia - NCAMs ) modulate cell - cell adhesive interactions involved in synaptogenesis , neural plasticity , myelination , and neural stem cell ( NSC ) proliferation and differentiation . -
pubmed
These differentiated cells were immunoreactive with mAb 's to
polySia ,
NCAM ,
beta - III tubulin ,
nestin ,
S-100 and stained with
BrdU. -
pubmed
Autosomal dominant hypercholesterolemia (
ADH ) characterized by markedly elevated
LDL cholesterol levels that leads to premature morbidity and mortality from
atherosclerotic cardiovascular disease (
ASCVD ) . -
pubmed
Familial combined hyperlipidemia (
FCHL ) characterized by variable elevations of total
cholesterol ,
triglycerides , or
LDL cholesterol and a high risk of premature
ASCVD . -
pubmed
Pregnancy management : In a woman with the
APOE p. Leu167del variant ,
triglyceride levels should be measured when pregnancy is identified and at least one to two times later in pregnancy as severe
hypertriglyceridemia ( & gt;500 mg / dL ) in pregnancy increases the risk for
pancreatitis and fetal and
maternal death . -
pubmed
Acetylcholinesterase is a
serine protease that rapidly hydrolyzes neurotransmitter
acetylcholine . -
pubmed
Their implications were studied using specific inhibitors (
U0126 ,
mitogen - activated protein kinase kinase ,
MEK , inhibitor and C compound ,
AMPK inhibitor ) . -
pubmed
Both
U0126 and C compound almost completely prevented LPS - induced
TNF - alpha production . -
pubmed
INTRODUCTION : Both
testosterone therapy and chronic treatment with
phosphodiesterase type 5 inhibitors ( PDE5Is ) have positive effects on the histology of penile corpora and erectile function . -
pubmed
Inhibition of calpain prevents
manganese - induced cell
injury and
alpha - synuclein oligomerization in organotypic brain slice cultures . -
pubmed
Overexposure to
manganese has been known to promote
alpha - synuclein oligomerization and enhance cellular toxicity . -
pubmed
However , the exact mechanism of
Mn - induced
alpha - synuclein oligomerization is unclear . -
pubmed
After slices were treated with 400 muM
Mn for 24 h , there were significant increases in the percentage of apoptotic cells ,
lactate dehydrogenase release , intracellular [
Ca2+]i ,
calpain activity , and the mRNA and protein expression of
calpain 1 and
alpha - synuclein . -
pubmed
These results also showed that
calpain inhibitor II pretreatment could reduce
Mn - induced
nerve cell injury and
alpha - synuclein oligomerization . -
pubmed
These findings revealed that
Mn induced the cleavage of
alpha - synuclein protein via overactivation of
calpain and subsequent
alpha - synuclein oligomerization in cultured slices . -
pubmed
Moreover , the cleavage of
alpha - synuclein by
calpain 1 is an important signaling event in
Mn - induced
alpha - synuclein oligomerization . -
pubmed
The Full Scale IQ was not significantly different between groups ( high -
DHA 98.3 , SD 14.0 , standard -
DHA 98.5 ,
SD 14.9 ; mean difference adjusted for sex , birthweight strata and hospital -0.3 , 95 % CI -2.9 to 2.2 ; p=0.79 ) . -
pubmed
Click Chemistry in Lead Optimization of
Boronic Acids as
beta - Lactamase Inhibitors . -
pubmed
Boronic acid transition - state inhibitors ( BATSIs ) represent one of the most promising classes of
beta - lactamase inhibitors . -
pubmed
This synthetic pathway , which avoids intermediate purifications , proved to be flexible and efficient , affording in good yields a panel of 14 BATSIs bearing three different
R1 amide side chains (
acetamido ,
benzylamido , and
2 - thienylacetamido ) and several R substituents on the
triazole . -
pubmed
Homology modeled structure analysis demonstrated that
cecropin - TY1 adopted two alpha - helices (
Leu3 -
Thr24 ,
Ile27 -
Leu38 ) linked by a hinge (
Leu25 -
Pro26 ) and the structure surface was partly positively charged . -
pubmed
METHODS : Fourteen population clusters of approximately 1000 residents each in Zambia 's Luangwa and Nyimba districts , which had high pre - existing usage rates ( 81.7 % ) of
pyrethroid - impregnated LLINs were quasi - randomly assigned to receive IRS with either of two
pyrethroids , namely
deltamethrin [ Wetable granules (
WG ) ] and
lambdacyhalothrin [ capsule suspension (
CS ) ] , with an emulsifiable concentrate ( EC ) or
CS formulation of the
organophosphate pirimiphos methyl (
PM ) , or with no supplementary vector control measure . -
pubmed
RESULTS : Supplementation of LLINs with PM
CS offered the greatest initial level of protection against
malaria in the first 3 months of application ( incremental protective efficacy ( IPE ) [ 95 % confidence interval ( CI ) ] = 0.63 [ CI 0.57 , 0.69 ] , P & lt ; 0.001 ) , followed by
lambdacyhalothrin (
IPE [ 95 % CI ] = 0.31 [ 0.10 , 0.47 ] , P = 0.006 ) and PM EC ( IPE , 0.23 [ CI 0.15 , 0.31 ] , P & lt ; 0.001 ) and then by
deltamethrin ( IPE [ 95 % CI ] = 0.19 [ -0.01 , 0.35 ] , P = 0.064 ) . -
pubmed
Neither
pyrethroid formulation provided protection beyond 3 months after spraying , but the protection provided by both PM formulations persisted undiminished for longer periods : 6 months for
CS and 12 months for EC . -
pubmed
The
CS formulation of PM provided greater protection than the combined
pyrethroid IRS formulations throughout its effective life IPE [ 95 % CI ] = 0.79 [ 0.75 , 0.83 ] over 6 months . -
pubmed
A key drug for treatment of
EGFR mutation - positive
non - small cell lung cancer is
epidermal growth factor receptor -
tyrosine kinase inhibitor (
EGFR - TKI ) . -
pubmed
The median progression - free survival in the
gefitinib group and the
erlotinib group was 22.4 months and
14.1 months , respectively , and the median overall survival was 30.5 months and 32.4 months , respectively . -
pubmed
Although
SOD and
GSH were significantly higher in spermatic vein compared to median cubital vein , TAC was significantly higher in median cubital vein . -
pubmed
Involvement of
GABAA receptors in
5 - HT1A and
sigma1 receptor synergism on prefrontal dopaminergic transmission under circulating
neurosteroid deficiency . -
pubmed
RATIONALE : We previously reported that the
fluvoxamine - induced increase in prefrontal
dopamine levels is enhanced by adrenalectomy / castration ( which results in circulating
neurosteroid deficiency ) , via combined activation of
serotonin1A ( 5 - HT1A ) and sigma1 receptors . -
pubmed
Picrotoxin also potentiated the increase in prefrontal
dopamine levels mediated by co - administration of the
5 - HT1A receptor agonist
osemozotan and the
sigma1 receptor agonist
( + ) - SKF-10,047 , while it did not affect the co - administration - induced changes in
noradrenaline and
serotonin levels . -
pubmed
Conversely , the
GABAA receptor agonist
diazepam ( 1 mg / kg ) blocked the effect of adrenalectomy / castration on the
fluvoxamine - induced increase in prefrontal
dopamine levels . -
pubmed
CONCLUSIONS : These results suggest that the
GABAA receptor plays a key role in mediating the synergistic effects of
5 - HT1A and
sigma1 receptor activation on prefrontal
dopamine neurotransmission . -
pubmed
In vivo PET / CLI imaging clearly reveals SLNs as early as 1 h
post PEG - RIe - AuNP - injection , with peak signals achieved at 6 h postinjection , which is consistent with the biodistribution results . -
pubmed
In vitro , like ERS inhibitor
4 - phenylbutyric acid (
PBA ) , HDL inhibited ox - LDL- or
tunicamycin (
TM , an ERS inducer)-induced increase in
visfatin and
resistin secretion . -
pubmed
Moreover ,
HDL inhibited ox - LDL - induced free
cholesterol ( FC ) accumulation in whole cell lysate and in the endoplasmic reticulum . -
pubmed
Additionally , like
PBA ,
HDL inhibited ox - LDL- or
TM - induced activation of ERS response as assessed by the decreased phosphorylation of
protein kinase - like ER kinase and
eukaryotic translation initiation factor 2alpha and reduced nuclear translocation of
activating transcription factor 6 as well as the downregulation of
Bip and
CHOP . -
pubmed
YB1 and
NOD2 co - migrated on
sucrose gradient centrifugation , and
GMDP addition led to the formation of higher molecular mass complexes containing both
YB1 and
NOD2 . -
pubmed
Co - immunoprecipitation demonstrated a direct interaction between
YB1 and
NOD2 , a major recombinant fragment of
NOD2 (
NACHT - LRR ) bound to
YB1 , and complex formation was stimulated by
GMDP . -
pubmed
A method based on the analysis of
trimethylsilyl (
TMS ) derivatives by capillary gas chromatography electrospray ionization mass spectrometry (
GC - ESI /
MS ) was proposed . -
pubmed
BACKGROUND :
Artemisinin combination therapy (
ACT ) is used worldwide as the first - line treatment against
uncomplicated Plasmodium falciparum malaria . -
pubmed
Despite the success of
ACT in reducing the global burden of
malaria , the emerging of resistance to
artemisinin threatens its use . -
pubmed
flux (
Y1 ) , lag time ( Y2 ) , diffusion coefficient (
Y3 ) , permeability coefficient (
Y4 ) , and input rate (
Y5 ) of
clotrimazole gel formulations . -
pubmed
Moreover ,
PTS was able to increase capillary densities and enhance angiogenesis in ischemic boundary zones and up - regulate
vascular endothelial growth factor (
VEGF ) and
Angiopoietin-1 (
Ang-1 ) expression by activating the
Shh signaling pathway . -
pubmed
Here , structure - activity relationship of
rMnSODSeq was evaluated on the basis of its activity and stability in the presence of inhibitors ,
NaCl , denaturants , detergents , reducing agents , and at different pH values . -
pubmed
The distribution of brominated flame retardants ( BFRs ) including
8PBDEs ,
DBDPE ,
BTBPE , EH - TBB ,
BEH - TEBP and
PBEB in road dust (
RD ) collected in Xinxiang , China was characterized . -
pubmed
Of the 13 analyzed substances ,
BDE-209 and
BEH - TEBP were the most abundant components in
RD from Xinxiang . -
pubmed
Similar amounts of
BDEs excluding
BDE-209 were found at different types of sampling sites , and thus , atmospheric deposition is also a probable source of BFRs in
RD which can be subject to air transportation . -
pubmed
Our results confirm that the use of
deca - BDE commercial mixture is a major source of
PBDE contamination in
RD . -
pubmed
Cathepsins are lysosomal peptidases belonging to the papain family , and based on their catalytic sites , these enzymes can be divided into
serine ,
cysteine and
aspartic proteases . -
pubmed
The wear resistance of the
Zr -
Al -
Fe -
Nb alloys in air and
phosphate buffer saline (
PBS ) solution is superior to that of pure
Zr . -
pubmed
The wear mechanism of
Zr - based alloys sliding in air is controlled by oxidation and abrasive wear whereas that sliding in
PBS is controlled by synergistic effects of the abrasive and corrosive wear . -
pubmed
Electrochemical measurements demonstrate that the
Zr - based alloys are corrosion resistant in
PBS . -
pubmed
The
Zr75Al5Fe10Nb10 exhibits the best corrosion resistance in
PBS , which contributes to its superior wear resistance in a simulated body environment . -
pubmed
The potencies of
PFASs to inhibit
SRD5A1 are
PFOS & gt ;
PFOA & gt ;
PFHxS =
PFBS , with IC50 values of
PFOS and
PFOA of 1.92 + - 0.07 and 14.24 + - 0.07 muM and no effects for
PFHxS and
PFBS at 100 muM , respectively . -
pubmed
The potencies of
PFASs to inhibit
AKR1C9 and
RoDH2 are
PFOS & gt ;
PFOA & gt ;
PFHxS =
PFBS , with IC50 values of
PFOS and
PFOA on these two enzymes about 100 muM and no effects for
PFHxS and
PFBS at 100 muM , respectively . -
pubmed
Upon identifying a lead hit from this screen KEA1 - 97 , we used activity - based protein profiling ( ABPP)-based chemoproteomic platforms to identify that this compound targets
lysine 72 of
thioredoxin - a site previously shown to be important in protein interactions with
caspase 3 to inhibit
caspase 3 activity and suppress apoptosis . -
pubmed
It is classified as
MPS type III , though it is caused by four different
genetic defects , determining subtypes A , B , C and D. In each subtype of
MPS III , the primary storage GAG is
heparan sulfate ( HS ) , but mutations leading to
A , B , C , and D subtypes are located in genes coding for
heparan N - sulfatase ( the
SGSH gene ) ,
alpha - N - acetylglucosaminidase ( the
NAGLU gene ) ,
acetyl - CoA : alpha - glucosaminide acetyltransferase ( the
HGSNAT gene ) , and
N - acetylglucosamine-6 - sulfatase ( the
GNS gene ) , respectively . -
pubmed
DiR " hotspots " were specifically associated with
CD206 - expressing M2 macrophages in the spinal cord and this was confirmed by co - localization with anti -
CD63 antibodies labeling a tetraspanin characteristically expressed on exosomes . -
pubmed
Herein , we show that single intra - medial forebrain bundle ( MFB ) injection of
6 - hydroxydopamine (
6 - OHDA ) efficiently induced long - term activation of
GSK-3beta and reduced NSC self - renewal , proliferation ,
neuronal migration , and neuronal differentiation accompanied with increased astrogenesis in subventricular zone ( SVZ ) and hippocampal dentate gyrus ( DG ) . -
pubmed
Ethanol exposure inhibits hepatocyte lipophagy by inactivating the
small guanosine triphosphatase Rab7 . -
pubmed
Nutrient
starvation results in
Rab7 activation on the surface of the
LD and lysosomal compartments , resulting in the mobilization of
triglycerides stored within the LDs for energy production . -
pubmed
Here , we examine whether the steatotic effects of
alcohol exposure are a result of perturbations to the
Rab7 - mediated lipophagic pathway . -
pubmed
Conclusion : A mechanism is supported whereby
ethanol exposure inhibits
Rab7 activity , resulting in the impaired transport , targeting , and fusion of the autophagic machinery with LDs , leading to an accumulation of hepatocellular
lipids and
hepatic steatosis . -
pubmed
The interaction mechanism of
phenol and
PAHs in aqueous and sludge phases was systematically studied in two full - scale engineering projects composed of anaerobic - oxic - oxic ( A -
O1 -
O2 ) and anaerobic - oxic - hydrolytic - oxic ( A -
O1 - H - O2 ) sequences . -
pubmed
Thus , these results suggested that in vivo1H
MRS can be used to reliably investigate the
glutamate system . -
pubmed
The size of the
Protein Data Bank (
PDB ) has increased more than 15 - fold since 1999 , which enabled the expansion of models that aim at predicting enzymatic function via their
amino acid composition . -
pubmed
One such system is the standard genetic code (
SGC ) , which generally minimizes the cost of
amino acid replacements resulting from mutations or mistranslations . -
pubmed
This approach allowed us to place the
SGC in the global space of possible codes , which is a more appropriate and unbiased comparison than that with randomly generated codes because they are characterized by relatively uniform
amino acid assignments to codons . -
pubmed
Hyoscyamine is converted to
6beta - hydroxyhyoscyamine (
anisodamine ) and
scopolamine by
hyoscyamine 6beta - hydroxylase (
H6H ) , a
2 - oxoglutarate dependent dioxygenase . -
pubmed
Furthermore , four critical hormones of
salicylic acid (
SA ) ,
jasmonic acid ( JA ) ,
ethylene (
ET ) and
abscisic acid (
ABA ) had higher levels in 1/3TP , 2/3TP and
TP than those in
NP . -
pubmed
CONCLUSIONS : Tx -
ESRD patients have worse quality metrics related to
anemia ,
phosphorus ,
albumin , and vascular access compared to Nat -
ESRD patients . -
pubmed
He presented to us one year later with a right neck draining
sinus tract , reaching the carotid sheath on
CT scan . -
pubmed
RESULTS : All
melatonin treatments reduced
chilling injury symptoms , reduced
electrolyte leakage ,
malondialdehyde (
MDA ) content ,
H2 O2 and
superoxide anion (
O2 - ) , and increased
proline content and the activity of
superoxide dismutase (
SOD ) ,
catalase (
CAT ) , and
g - aminobutyric acid (
GABA ) , and reduced the activities of
phospholipase D (
PLD ) and
lipoxygenase (
LOX ) compared to the control . -
pubmed
Treatment with
oridonin for 24 h increased apoptosis by 4.1 % , and increased the protein levels of
Bax and
cleaved caspase-3 but significantly decreased the levels of
IL-1beta in the culture supernatant ( p & lt ; 0.05 ) . -
pubmed
In addition , 6 h of
oridonin treatment significantly decreased the number of GFP -
LC3 punctate dots and inhibited the protein levels of
ATG5 and
Beclin1 by 80.01 % and 42.12 % , respectively . -
pubmed
Acute inflammatory
pericarditis secondary to
cardiac sarcoidosis evaluated by
18F - fluorodeoxyglucose PET /
CT imaging . -
pubmed
DOX increased the
malondialdehyde level and decreased the
catalase ,
superoxide dismutase activities and
glutathione levels ; MP prevented these alterations . -
pubmed
The control of extracellular
ATP and
adenosine levels is done by
ectonucleotidases , which represent a potential target to be evaluated in the progression of
cardiovascular diseases . -
pubmed
AREAS COVERED : The fetal safety / risks of
clomiphene citrate ,
aromatase inhibitors ,
metformin ,
gonadotropins and
progestins are discussed . -
pubmed
In the multivariate analysis , increasing largest
tumor dimension ( p < 0.01 ) and
sinus invasion ( p < 0.01 ) predicted Simpson grade
IV resection , whereas skull base location predicted neurological morbidity ( p = 0.02 ) . -
pubmed
A RESPONSE TO " THE POTENTIAL LONG - TERM COMPARATIVE EFFECTIVENESS OF
LAROTRECTINIB AND
ENTRECTINIB FOR SECOND - LINE TREATMENT OF
TRK FUSION - POSITIVE
METASTATIC LUNG CANCER " . -
pubmed
These responses were secondary to
sPRR -
His evoked elevations in
Nox4 - derived
H2O2 production that resulted in
inflammation , apoptosis and reduced
NO production . -
pubmed
Each of these
sPRR - His - evoked responses was attenuated by
AT1R inhibition using
Losartan (
Los ) but not
ACE inhibition using
captopril (
Cap ) . -
pubmed
Further mechanistic exploration revealed that
sPRR -
His activated
AT1R downstream
Gq signaling pathway . -
pubmed
Immunoprecipitation coupled with autoradiography experiments and radioactive ligand competitive binding assays indicate
sPRR directly interacts with
AT1R via
Lysine199 and
Asparagine295 . -
pubmed
SRC family kinase (
SFK ) inhibitor
dasatinib improves the antitumor activity of
anti - PD-1 in
NSCLC models by inhibiting Treg cell conversion and proliferation . -
pubmed
In in vitro assays , we demonstrated that
dasatinib blocks proliferation and
transforming growth factor beta - driven conversion of effector
CD4 + cells into Tregs through targeting of
phospholymphocyte - specific protein tyrosine kinase and downstream effectors
pSTAT5 and
pSMAD3 . -
pubmed
Dasatinib synergizes with
anti - PD-1 to impair
tumor growth in
NSCLC experimental models . -
pubmed
A comparison of the substrate and electron - donor specificities of the
methane mono - oxygenases from three
strains of
methane - oxidizing bacteria . -
pubmed
The optimized energies of seven beta -
bends , repeating
C5 and
C7 , and right- and left - handed alpha - helical conformations for each of eight
tetrapeptides have been computed using empirical methods . -
pubmed
Activation of
bovine factor X in the presence of
calcium ,
magnesium ,
barium or
manganese ion . -
pubmed
Inhibition by
acetylene of conventional
hydrogenase in
nitrogen - fixing bacteria . -
pubmed
It outflowed from the determination of selected
serum proteins (
Ig and acute phase proteins ) and salivary
lysozyme that the group under the combined influence of
formaldehyde and
toluene showed significantly lower
IgG and higher
alpha-1 - antitrypsin (
A1AT ) . -
pubmed
The group at risk of
toluene was characteristical in elevated concentrations of
alpha-2 - macroglobulin (
A2 M ) and
A1AT . -
pubmed
Salivary
lysozyme concentration of apprentice environmentally exposed to
formaldehyde in the noon showed the decrease , whereas its increase occurred in controls with the difference on 5 % significancy level . -
pubmed
Serum
triglycerides and lipoprotein
cholesterol were measured before and after the
trip . -
pubmed
With the four different diets used , a standard diet ( 3800 kcal day-1 , 26 % fat , 260 mg
cholesterol day-1 ) , a low - energy diet ( 2300 kcal day-1 , 21 % fat , 110 mg
cholesterol day-1 , a high - fat - high -
cholesterol diet ( 3800 kcal day-1 , 52 % fat , 480 mg
cholesterol day-1 ) and a high -
cholesterol diet ( 3800 kcal day-1 , 29 % fat , 410 mg
cholesterol day-1 ) , a decrease in
very low - density lipoprotein -
low - density lipoprotein (
VLDL - LDL ) fraction by 38 % , 50 % , 41 % , and 54 % , respectively , was obtained . -
pubmed
No significant increase in
high - density lipoprotein (
HDL )
cholesterol was found in the experiments with the standard diet and the low - energy diet , but in the high - fat - high -
cholesterol diet experiment the
HDL cholesterol increased by 19 % and the ratio
HDL cholesterol : total
cholesterol from 0.327 to 0.490 . -
pubmed
With the high -
cholesterol diet an increase in
HDL cholesterol of about the same extent was obtained . -
pubmed
Titration of the average
Ca2 + concentration showed that the
Ca2 + affinity for
cholecystokinin-8 secretion was higher than that for
amino acids . -
pubmed
The predicted
v - rel protein (
p59v -
rel ) has 14
amino acid substitutions and 3
amino acid deletions relative to the predicted
turkey c - rel protein . -
pubmed
The 5 '
Rev env - derived amino - terminus of
p59v -
rel also has
amino acid substitutions in codons 3 , 6 , and 9 relative to
wild - type Rev - A env . -
pubmed
To distinguish the critical alterations from the neutral ones , we made a mutation that resulted in a substitution of the
5 ' Rev env - derived amino - terminus by a single
methionine , as well as several back - mutations , in the context of an otherwise wild - type
v - rel and tested the transforming , immortalizing , and tumorigenic properties of the resulting proteins . -
pubmed
Absence of the
5 ' Rev env - derived amino - terminus , as well as back - mutations of six of the
amino acid changes present in the
rel - derived
amino - terminal and central regions of
v - rel , reduced transformation efficiency . -
pubmed
IL-1 beta ( 0.3 micrograms / kg ) administered 24 h before induction of
colitis increased
PGE2 ( 231 + /- 36 to 1,299 + /- 572 pg / ml , P less than 0.01 ) and reduced subsequent inflammatory cell infiltration index ( from 2.8 + /- 0.3 to 1.4 + /- 0.3 , P less than 0.02 ) and
edema ( from 2.5 + /- 0.3 to 1.3 + /- 0.3 , P less than 0.01 ) compared with vehicle - matched animals . -
pubmed
together with
IL-1 beta prevented the stimulation of
PGE2 and the reduction in
inflammation . -
pubmed
These data demonstrate that pretreatment with
IL-1 beta 24 h before the induction of
colitis reduces
inflammation by a mechanism that requires
prostaglandin synthesis . -
pubmed
Since BM 42.304 decreased mitochondrial
acylcarnitine and increased the cytosolic concentration significantly , BM 42.304 was presumed to inhibit mitochondrial
acylcarnitine translocase . -
pubmed
This effect of
aprotinin may be a consequence of : renal
kallikrein inhibition which prevents augmentation of
renal kinins in response to increased
vasopressin levels , or other unrecognized properties of
aprotinin . -
pubmed
2 urine samples collected 10 and 13 days after
Glucantime treatment revealed only
antibody (
IgG ) , not soluble antigen . -
pubmed
Direct measurements of phosphorylation of the
Ca2 + ATPase of the sarcoplasmic reticulum (
SR ) have shown that the lifetime of the first phosphorylated intermediate in the
Ca2 + transport cycle ,
E1 approximately P , increases with decreasing [
Mg2 + ] ( Dupont , Y. -
pubmed
54:669 - 678 ) under high [
Mg2 + ] conditions ( 25 mM ) indicated that changes in the profile structure of the
SR membrane could be responsible for the low - temperature transient trapping of
E1 approximately P that occurs at temperatures below 2 - 3 degrees C , the upper characteristic temperature th for
lipid lateral phase separation in the membrane . -
pubmed
We now present results of our study of the
Ca2 + uptake kinetics and of the structure of the
SR membrane at low [
Mg2 + ] ( less than or equal to 100 microM ) . -
pubmed
Our results show a slowing in the kinetics of both phases of the
Ca2 + uptake process and an increase in the duration of the plateau of the fast phase before the onset of the slow phase , indicating an increase in the lifetime ( transient trapping ) of
E1 approximately P.
Calcium uptake kinetics at low [
Mg2 + ] and moderately low temperature ( approximately 0 degree C ) are similar to those observed at much lower temperatures ( approximately -10 degrees C ) at high [
Mg2 + ] . -
pubmed
Also , at the lower [
Mg2 + ] the upper characteristic temperature th for lipid lateral phase separation was found to be higher , at approximately 8 - 10 degrees C. Our studies indicate that both temperature and [
Mg2 + ] affect the structure and the functionality ( as measured by changes in the kinetics of
Ca2 + uptake ) of the
SR membrane . -
pubmed
Membrane lipid phase behavior and changes in the
Ca2 + ATPase profile structure seem to be related , and we have found that structural changes are responsible for the slowing of the kinetics of the fast phase of
Ca2 + uptake , and could also mediate the effect that [
Mg2 + ] has on
E1 approximately P lifetime . -
pubmed
Urine was collected in several fractions on each study day and the urine volume , excretion of
ofloxacin , and excretion of
creatinine ,
alanine aminopeptidase ,
N - acetyl - beta - D - glucosaminidase , and
gamma - glutamyltransferase were calculated in each fraction collected . -
pubmed
The effects of
hydroxyurea have been investigated on three events of the cell cycle , S - phase , mitosis , and the cyclic synthesis of
thymidine kinase , in the synchronous plasmodium of the myxomycete Physarum . -
pubmed
Hydrolysis with
phospholipase established that
phytanate was confined to the 1 - position of
CGP . -
pubmed
The effect of
urea on salt - induced changes in
histone IV . -
pubmed
The effect on
actin ATPase of
phalloidin and
tetramethylrhodamine phalloidin . -
pubmed
Actin polymerization has been studied in the absence of excess
nucleotide . -
pubmed
Using
G - actin ATP monomers , it was shown that mechanical shearing stimulates
ATP hydrolysis . -
pubmed
The procedures used enabled the detection of differential effects of
phalloidin and
tetramethylrhodamine - phalloidin , on the P(i)-release step of the
actin ATPase . -
pubmed
It is concluded that
tetramethylrhodamine , in contrast to
phalloidin , accelerates P(i)-release from
actin filaments . -
pubmed
These tissues require
insulin for facilitated transport of
glucose and express the
insulin - responsive transporter
GLUT4 . -
pubmed
The present results indicate that simultaneous measurement of the levels of urinary
gamma - Gla and plasma
prothrombin and
protein C is a useful tool for the diagnosis of
liver diseases and
diabetes mellitus . -
pubmed
This in vitro release of
LAC was abolished when samples were stored on ice until centrifugation within 5 h. In contrast , a statistically significant increase in
ELA - PI of 10 % was observed following storage on ice for 2 h of blood specimens drawn from healthy persons . -
pubmed
EDTA - plasma obtained by venous puncture following minimal stasis contained 10 % higher concentrations of
LAC compared to samples drawn from intravenous catheters , while no difference was observed in the case of ELA -
PI . -
pubmed
A structure - activity study of
fatty acid interaction with
mitochondrial uncoupling protein . -
pubmed
Fatty acid (
FA ) uniport via mitochondrial
uncoupling protein (
UcP ) was detected fluorometrically with
PBFI ,
potassium - binding
benzofuran phthalate and
SPQ ,
6 - methoxy - N -( 3 - sulfopropyl )- quinolinium , indicating K+ and
H + , respectively . -
pubmed
The
FA structural patterns required for
FA flip - flop ,
UcP - mediated FA uniport , activation of
UcP - mediated
H + transport in proteoliposomes , and inhibition of
UcP - mediated
Cl- uniport by
FA , were identical . -
pubmed
DMXB ( 100 microM ) activated
alpha7 homo - oligomeric receptors , without significant activity at alpha2- , alpha3- and
alpha4 - containing subtypes . -
pubmed
Iron contents of the brain , activities of
monoamine oxidase (
MAO ) in the corpus striatum , and the contents of
monoamine neurotransmitter and its metabolite in the cerebral cortex and hippocampus were determined by DCP - AES technique , enzyme histochemical method , and high performance liquid chromatography with electrochemical detection ( HPLC -
ECD ) , respectively . -
pubmed
Modulation of the cellular metabolism of
cytarabine and
fludarabine by
granulocyte - colony - stimulating factor during therapy of
acute myelogenous leukemia . -
pubmed
Previous in vitro investigations demonstrated that
human leukemia cells , when incubated with
hematopoietic growth factors such as
granulocyte - colony - stimulating factor (
G - CSF ) , augment the accumulation of the
triphosphate 1 - beta - D - arabinofuranosylcytosine (
ara - C cytarabine ) . -
pubmed
To test whether
G - CSF infusion prior to
ara - C infusion would biologically modulate the accumulation of
ara-9 - beta - D - arabinofuranosylcytosine 5 '- triphosphate (
ara - CTP ) and other
ara nucleotides in the
leukemia blasts during therapy , protocols were designed to infuse
G - CSF prior to
fludarabine (
9 - beta - D - arabinofuranosyl-2 - fluoroadenine monophosphate ) and
ara - C to increase the accumulation of the active
triphosphates [
9 - beta - D - arabinofuranosyl-2 - fluoroadenine 5 '- triphosphate (
F - ara - ATP ) and
ara - CTP ] in
acute myelogenous leukemia (
AML ) blasts during therapy . -
pubmed
To complement these in vivo studies , ex vivo accumulation of
ara - CTP was also investigated before and after
G - CSF infusion . -
pubmed
The AUC of
ara - CTP , on the other hand , was not affected ( median , 1.0 - fold ; range , 1.0 - 1.2 ; P = 0.571 ) after
G - CSF infusion . -
pubmed
Consistent with these in vivo investigations , ex vivo
ara - CTP accumulation was decreased in the
AML blasts after
G - CSF infusion . -
pubmed
Based on these data it could be concluded that ( a ) infusion of
G - CSF before
fludarabine augmented the rate of
F - ara - ATP synthesis in circulating
AML blasts during therapy , suggesting that
G - CSF may benefit
fludarabine therapy by biological modulation ; ( b )
G - CSF did not increase
ara - CTP accumulation , rather it may have caused it to decrease ; and ( c ) these data imply that when
G - CSF and
ara - C are used in combination , administration of
fludarabine prior to
ara - C may maintain the
ara - CTP AUC . -
pubmed
The structure of
tubulin shows
paclitaxel (
Taxol(R ) ) binding to a pocket in
beta tubulin on the microtubule 's inner surface , which counteracts the effects of
GTP hydrolysis occurring on the other side of the monomer . -
pubmed
The re - activated
I ( min ) had the same kinetic properties as I(min ) when it is activated by
Ca ( 2 +)-mobilizing agonists in intact cells and by
IP ( 3 ) in excised plasma membrane patches and it was inhibited by the
I ( crac ) inhibitor
SKF95365 . -
pubmed
These data support the possible role of
SHMT in the fetal control of plasma
serine levels . -
pubmed
While
cSHMT may contribute to fetal hepatic
serine production , its activity pattern does not support a primary role in the control of fetal hepatic
serine biosynthesis . -
pubmed
In the placenta ,
mSHMT may be important for
glycine production from
serine . -
pubmed
The twin
arginine motif in its signal peptide and its sole integration requirement of a
DeltapH suggested that plastid fusion / protein translocation factor employs the
DeltapH pathway . -
pubmed
Thus ,
DeltapH pathway machinery facilitates both signal peptide - directed and N - tail - mediated membrane integration and does not strictly require the twin
arginine motif . -
pubmed
Carbamoyl phosphate synthetase (
CPS ) catalyzes one of the most remarkable reactions ever described in biological chemistry , in which
carbamoyl phosphate is produced from one molecule of
bicarbonate , two molecules of
Mg2 +
ATP , and one molecule of either
glutamine or
ammonia . -
pubmed
Instead it is the composition of the
coat protein domain from
amino acid 123 to 149 , as a minimal transmission domain , with the contribution of
amino acids 149 to 174 for efficient transmission . -
pubmed
UL97 is able to autophosphorylate and to phosphorylate certain exogenous substrates , including
nucleoside analogs such as
ganciclovir . -
pubmed
It has previously been concluded that phosphorylation of
UL97 is essential for its phosphorylation of
ganciclovir . -
pubmed
Glutathione S - transferase -
UL97 fusion protein purified from insect cells was found to be already partially phosphorylated , but neither extensive autophosphorylation nor
phosphatase treatment meaningfully altered the time course of its phosphorylation of the exogenous substrate ,
histone H2B. Sequencing and mass spectrometric analyses of
( 32 ) P - labeled tryptic peptides of the
UL97 fusion protein identified nine sites of autophosphorylation , all within the first 200 residues of the protein , outside of conserved protein kinase subdomains . -
pubmed
Types 1 and 2
11beta - HSD messenger RNA ( mRNA ) and protein in placentas obtained at 121 to 185 days ' gestation ( dGA , term approximately 185 dGA , n = 16 ) , during labor between 141 and 193 dGA ( n = 8) , and after maternal administration of four doses of 87.5 microg / kg
betamethasone ( n = 5 ) at 12 - hour intervals at 121 to 135 dGA were analyzed by Northern and Western blot . -
pubmed
Maternal
betamethasone administration dramatically increased ( P & lt;.05 )
11beta - HSD 2 mRNA as well as protein without effect on
11beta - HSD 1 mRNA and protein expression . -
pubmed
Decreased placental
11beta - HSD 2 may enhance maternal
cortisol passage to the fetus at the end of gestation , thereby contributing to
cortisol - mediated changes within the placenta and
cortisol in fetal plasma at this stage of fetal development . -
pubmed
The positive effect of
betamethasone on placental
11beta - HSD 2 induction further suggests an ability of the placenta to regulate glucocorticoid transfer in the presence of elevated maternal glucocorticoid . -
pubmed
27 and 28 were analyzed for
2BTCA using a solid - phase microextraction -
GC -
MS method . -
pubmed
The increase in
invertase activity coincided with the rapid accumulation of
glucose and
fructose that reached 8 - fold the control leaf value . -
pubmed
In addition , acid vacuolar
invertase activity appeared to be highly correlated with xylem sap
ABA concentration . -
pubmed
In order to investigate the nature of the relationship between
ABA and
invertase activity , and to disconnect
ABA from a likely
sucrose side - effect , excised leaves were supplied with
ABA or
sucrose . -
pubmed
As a consequence of
ABA supply , a peak in leaf
ABA appeared 4 h later which was followed by an enhancement of
vacuolar invertase activity . -
pubmed
The transcript level of the
Ivr2 gene encoding one
vacuolar invertase presented the same two peaks pattern as leaf
ABA , with a 2 h lag . -
pubmed
Thus ,
ABA appeared to be a powerful enhancer of the
IVR2 vacuolar invertase activity and expression . -
pubmed
A COMPONENT SPLITTING
DIISOPROPYLFLUOROPHOSPHATE IN PREPARATIONS OF
SENDAI AND NEWCASTLE DISEASE VIRUSES AND IN A FRACTION FROM NORMAL CHORIOALLANTOIC MEMBRANE CELLS ; ITS POSSIBLE IDENTITY WITH
HAEMOLYSIN . -
pubmed
AIMS : To determine the cost to the
NHS of prescribed low - dose
aspirin . -
pubmed
Serious adverse events were uncommon but despite this
aspirin cost the
NHS between 6 and 25 times the cost of
aspirin tablets due to dispensing costs and the cost of managing adverse effects . -
pubmed
The effects of
GABA and
GABAa receptor antagonist were observed in this experimental study . -
pubmed
Numerous histological and electrophysiological studies have been performed on the contribution of
GABA to the auditory pathway ; however , the spatio - temporal patterns of excitatory propagation and the relationships between
GABA receptor and excitatory propagation have yet to be reported . -
pubmed
Bicuculline methiodide (
BMI ) , a competitive antagonist against
GABAa receptor , partially reversed the effects of
GABA on the optical signals . -
pubmed
4,4 '- Dithiodipyridine (
PySSPy ) monolayers on
Au ( 111 ) were investigated by cyclic voltammetry , X - ray photoelectron spectroscopy ( XPS ) and in situ scanning tunneling microscopy (
STM ) . -
pubmed
Metoprolol and
propranolol (
BCS class I ) as well as
hydrochlorothiazide (
BCS class III ) were selected as model drugs . -
pubmed
The effect of
methyl viologen on the flux of the intermediate compounds was investigated by measuring the activity of
enzymes related to
PHB biosynthesis ,
glucose 6 - phosphate dehydrogenase (
G6PD ) , and
NADPH level . -
pubmed
Instead , a
threonine residue at position 15 in the
hKv1.5 N - terminus is critically important . -
pubmed
Mutation of this residue , which lies within a consensus site for phosphorylation by
protein kinase C , to an
alanine , completely abrogated the effect of
SAP97 on channel expression . -
pubmed
Although we were unable to detect phosphorylation of this residue , specific inhibition of
kinase C by
Calphostin C eliminated the increase in wild - type
hKv1.5 currents associated with
SAP97 overexpression suggesting a role for this
kinase in the response . -
pubmed
Dye - coupling analysis revealed that each mutant exhibits a dominant - negative effect on wild - type
Cx43 . -
pubmed
Caspase-3- and calpain - mediated
tau cleavage are differentially prevented by
estrogen and
testosterone in
beta - amyloid - treated hippocampal neurons . -
pubmed
Our results indicated that
estrogen and
testosterone prevented
caspase-3- and calpain - mediated
tau cleavage , respectively . -
pubmed
Thus ,
estrogen decreased the levels of
caspase-3 - cleaved 50 - kDa truncated
tau , while
testosterone prevented the generation of a
calpain - cleaved 17 - kDa
tau fragment . -
pubmed
Furthermore , our findings indicated that
testosterone was more effective than
estrogen in protecting hippocampal neurons against
Abeta - induced cell
death . -
pubmed
Collectively , our data suggest that preventing the decline of
estrogen and
testosterone associated with normal aging might reduce the susceptibility of central neurons to
Abeta - induced
toxicity . -
pubmed
Association between
cutaneous melanoma , Breslow thickness and
vitamin D receptor
BsmI polymorphism . -
pubmed
BrdU incorporation indicated that cell expansion occurred at multiple stages except
SP1 . -
pubmed
In addition ,
PCA also significantly decreased the activity of elevated
caspase-3 , indicating that
PCA may inhibit apoptosis of NSCs via suppression of the
caspase cascade . -
pubmed
Knockdown of
TxnIP with small interference RNA suggested that
TxnIP mediates the
glucose - induced impairment of
thioredoxin activity . -
pubmed
Knockdown of
TxnIP also abrogated both
glucose - induced
3H - proline incorporation ( a marker of
collagen production ) and oxidative stress . -
pubmed
Arginine methylation is a widespread
post - translational modification of proteins catalyzed by a family of
protein arginine methyltransferases (
PRMTs ) . -
pubmed
Trypanosomatids lack gene regulation at the level of transcription , instead relying on
post - transcriptional control mechanisms that act at the levels of RNA turnover , translation , and editing , all processes that likely involve multiple RNA - binding proteins , which are common targets of
arginine methylation . -
pubmed
High resolution ion exchange chromatography analysis of methylated substrates reveals that
TbPRMT7 is a
type III PRMT , catalyzing the formation of only
monomethylarginine , thereby representing the only exclusively type III
PRMT identified to date . -
pubmed
RESULTS :
Steatosis was associated significantly with donor age , increased
body mass index (
BMI ) , presence of
type II diabetes , ultrasonographic features , heavy
alcohol consumption , transaminases [
aspartate - aminotransferase and
alanine - aminotransferase (
ALT ) ] ,
gamma - glutamyl - transpeptidase , and
glucose blood levels . -
pubmed
The combination of
BMI , elevation of
ALT , presence of
type II diabetes , history of heavy
alcohol consumption , and ultrasonography signs of
steatosis could identify
steatosis & gt;30 % accurately with an AUC of 0.86 ( 95 % CI = 0.81 - 0.91 ) . -
pubmed
The effect of
dobutamine on AFC and the expression of
aquaporin-1 and
aquaporin-5 were examined . -
pubmed
Lipopolysaccharide administration results in significant
lung injury with impaired AFC , while
dobutamine improves alveolar fluid reabsorption with elevation of
aquaporin-1 and
aquaporin-5 . -
pubmed
Our study indicates that
dobutamine may enhance alveolar fluid reabsorption by increasing the expression of
aquaporin-1 and
aquaporin-5 . -
pubmed
Rosuvastatin ,
C - reactive protein ,
LDL cholesterol , and the JUPITER trial . -
pubmed
Pumilio 2 controls translation by competing with
eIF4E for
7 - methyl guanosine cap recognition . -
pubmed
Using deletion analysis , we have mapped the cap interaction domain of
Pum2 to the amino terminus of the protein and identified a conserved
tryptophan residue that mediates this specific interaction . -
pubmed
Human serum albumin consists of a single polypeptide of 585
amino acid residues with 1
Trp residue . -
pubmed
CONCLUSION : Despite the selection of
tumors expressing
c - Kit ,
imatinib did not appear to delay
disease progression after response to chemotherapy . -
pubmed
Structural role of the conserved
cysteines in the dimerization of the viral transmembrane oncoprotein
E5 . -
pubmed
For productive interaction ,
E5 needs to dimerize via a C - terminal pair of
cysteines , though a recent study suggested that its truncated transmembrane segment can dimerize on its own . -
pubmed
Comparison by circular dichroism in detergent micelles and lipid vesicular dispersion and by NMR in
trifluoroethanol demonstrates that the absence of one or both
cysteines does not influence the highly alpha - helical secondary structure , nor does it impair the ability of
E5 to dimerize , observations that are further supported by
sodium dodecylsulfate polyacrylamide gel electrophoresis . -
pubmed
Oriented circular dichroism in lipid bilayers shows that
E5 is aligned as a transmembrane helix with a slight tilt angle , and that this membrane alignment is also independent of any
cysteines . -
pubmed
Vitamin A directly correlated with
pre - albumin levels at 6 weeks ( r = 0.67 , P & lt ; 0.001 ) and 1 - year ( r = 0.67 , P & lt ; 0.0001 ) . -
pubmed
There was no correlation between the roux limb length measurement and
pre - albumin or
vitamin A serum concentrations at these
post - operative follow - ups . -
pubmed
Administration of
Gd - DPTA resulted in margin enhancement on
T1 - weighted images . -
pubmed
Mitoxantrone prior to
interferon beta-1b in aggressive
relapsing multiple sclerosis : a 3 - year randomised trial . -
pubmed
RESULTS : The time to worsen by at least one Expanded Disability Status Scale point confirmed at 3 months was delayed by 18 months in the
MITOX group compared with the
IFN group ( p<0.012 ) . -
pubmed
The 3 - year risk of worsening disability was reduced by 65 % in the
MITOX group relative to the
IFN group ( 11.8 % vs 33.6 % ) . -
pubmed
CONCLUSIONS : Although there were limitations in this investigator - academic - driven study , the data do suggest that
mitoxantrone induction therapy prior to
INF beta-1b may have a role in aggressive disease . -
pubmed
We fabricated [
Co /
Cu ] multilayers revealing a giant magnetoresistance (
GMR ) effect on free - standing elastic
poly ( dimethylsiloxane ) (
PDMS ) membranes . -
pubmed
The
GMR performance of [
Co /
Cu ] multilayers on rigid
silicon and on free - standing
PDMS is similar and does not change with tensile deformations up to 4.5 % . -
pubmed
This remarkable performance upon stretching relies on a wrinkling of
GMR layers on top of the
PDMS membrane . -
pubmed
The stimulatory
G protein alpha - subunit (
G ( s ) alpha ) couples hormone and other receptors to the generation of intracellular
cAMP . -
pubmed
Thus , excess GLP-1 action ( or at least through
GLP-1R ) does not contribute to the LGsKO metabolic phenotype , and other unknown factors involved in the cross talk between the liver G(s)alpha /
cAMP pathway and pancreatic islet function need to be further elucidated . -
pubmed
Taken together ,
MD-2 is a molecular target in the anti - inflammatory action of
1D10G. -
pubmed
The lowest cut - off value derived from receiving operator characteristic discriminating Serie A - B from
Lega - Pro was 1300 m. The ARIET performance was significantly correlated with VO(2max ) ( r = 0.78 , P & lt ; 0.001 ) , % HR(max ) after 4 min of ARIET ( r = - 0.81 , P & lt ; 0.001 ) and Yo - Yo
IR1 performance ( r = 0.95 , P & lt ; 0.001 ) , but not sprint performance ( r = -0.15 ; P = 0.58 ) . -
pubmed
Furthermore , middle and high
NaF significantly reduced the mRNA expressions of
P1 and
P2 , and
P1 / P2 ratio , whereas the sperm histones level was increased , suggesting the abnormal
histone - protamine replacement . -
pubmed
When the inhibitory role of
chemerin on steroidogenesis was further examined in vitro ,
chemerin suppressed
FSH - induced
progesterone and
estradiol secretion in cultured preantral follicles and granulosa cells . -
pubmed
( + ) - SKF 10047 (
N - allyl - normetazocine ) is a prototypic and specific
sigma-1 receptor agonist that has been used extensively to study the function of
sigma-1 receptors . -
pubmed
( + ) - SKF 10047 inhibits
K (+ ) ,
Na (+ ) and
Ca2 + channels via
sigma-1 receptor activation . -
pubmed
We found that
( + ) - SKF 10047 inhibited
Na ( V ) 1.2 and
Na ( V ) 1.4 channels independently of
sigma-1 receptor activation . -
pubmed
The
sigma-1 receptor antagonists
BD 1063,BD 1047 and
NE-100 did not block the inhibitory effects of
( + ) - SKF-10047 . -
pubmed
Blocking of the
PKA ,
PKC and
G - protein pathways did not affect
( + ) - SKF 10047 inhibition of
Na ( V ) 1.2 channel currents . -
pubmed
The
sigma-1 receptor agonists
Dextromethorphan ( DM ) and
1,3 - di - o - tolyl - guanidine (
DTG ) also inhibited
Na ( V ) 1.2 currents through a
sigma-1 receptor - independent pathway . -
pubmed
The
( + ) - SKF 10047 inhibition of
Na ( V ) 1.2 currents was use- and frequency - dependent . -
pubmed
Point mutations demonstrated the importance of
Phe(1764 ) and
Tyr(1771 ) in the
IV - segment 6 domain of the
Na ( V ) 1.2 channel and
Phe(1579 ) in the
Na ( V ) 1.4 channel for
( + ) - SKF 10047 inhibition . -
pubmed
AIM : Previous studies have shown that
uric acid ( UA ) has strong anti - oxidant properties , and that high circulating levels of UA are prospectively associated with improved muscle function and cognitive performances in elderly Caucasians . -
pubmed
CONCLUSION : The observed differences among the
cytotoxicity of
AH Plus ,
EndoREZ , and Epiphany did not reach a significant level at comparable time points after exposure . -
pubmed
The
CYP2C19 * 17 variant is not independently associated with
clopidogrel response . -
pubmed
BACKGROUND :
Cytochrome P450 2C19 (
CYP2C19 ) is the principal enzyme responsible for converting
clopidogrel into its active metabolite , and common genetic variants have been identified , most notably
CYP2C19 * 2 and
CYP2C19 * 17 , that are believed to alter its activity and expression , respectively . -
pubmed
OBJECTIVE : We evaluated whether the consequences of the
CYP2C19 * 2 and
CYP2C19 * 17 variants on
clopidogrel response were independent of each other or genetically linked through linkage disequilibrium (
LD ) . -
pubmed
PATIENTS / METHODS : We genotyped the
CYP2C19 * 2 and
CYP2C19 * 17 variants in 621 members of the Pharmacogenomics of Anti - Platelet Intervention ( PAPI ) Study and evaluated the effects of these polymorphisms singly and then jointly , taking into account
LD , on
clopidogrel prodrug level ,
clopidogrel active metabolite level , and
adenosine 5 '- diphosphate (
ADP)-stimulated
platelet aggregation before and after
clopidogrel exposure . -
pubmed
In association analyses that did and did not account for the effects of
CYP2C19 * 17 ,
CYP2C19 * 2 was strongly associated with levels of
clopidogrel active metabolite ( beta = -5.24 , P = 3.0 x 10(-9 ) and beta = -5.36 , P = 3.3 x 10(-14 ) , respectively ) and posttreatment
ADP - stimulated
platelet aggregation ( beta = 7.55 , P = 2.9 x 10(-16 ) and beta = 7.51 , P = 7.0 x 10(-15 ) , respectively ) . -
pubmed
In contrast ,
CYP2C19 * 17 was marginally associated with
clopidogrel active metabolite levels and
ADP - stimulated
platelet aggregation before ( beta = 1.57 , P = 0.04 and beta = -1.98 , P = 0.01 , respectively ) but not after ( beta = 0.40 , P = 0.59 and beta = -0.13 , P = 0.69 , respectively ) adjustment for the
CYP2C19 * 2 variant . -
pubmed
Stratified analyses of
CYP2C19 * 2 /
CYP2C19 * 17 genotype combinations revealed that
CYP2C19 * 2 , and not
CYP2C19 * 17 , was the primary determinant in altering
clopidogrel response . -
pubmed
CONCLUSIONS : Our results suggest that
CYP2C19 * 17 has a small ( if any ) effect on
clopidogrel - related traits and that the observed effect of this variant is due to
LD with the
CYP2C19 * 2 loss - of - function variant . -
pubmed
Magnetic particle imaging (
MPI ) is a novel tracer - based imaging method detecting the distribution of superparamagnetic
iron oxide ( SPIO ) nanoparticles in vivo in three dimensions and in real time . -
pubmed
The purpose of this study was to evaluate the angiogenic effect of topical application of three types of
ONO-1301 - loaded
poly ( lactide - co - glycolide ) microspheres ( ONO-1301
PLGA MS ) . -
pubmed
ONO-1301
PLGA MS were prepared with
PLGA 5010 , 5020 and 5050 ( with molecular weights of 10 K , 20 K and 50 K , respectively ) , using the solvent evaporation method . -
pubmed
Additional in vivo fluorescence imaging showed that
PLGA MS remained in the sponge for 28 days . -
pubmed
Luminal nutrient sensing by
G - protein - coupled receptors (
GPCR ) expressed on the apical domain of enteroendocrine cells activates intracellular pathways leading to secretion of gut hormones that control vital physiological processes such as digestion , absorption , food intake and
glucose homeostasis . -
pubmed
It has been shown that
T1R2 -
T1R3 , in association with
G - protein gustducin , is expressed in intestinal K and L endocrine cells , where it acts as the intestinal
glucose ( sweet ) sensor . -
pubmed
A number of studies have demonstrated that activation of
T1R2 -
T1R3 by natural
sugars and artificial sweeteners leads to secretion of
glucagon - like peptides 1&2 (
GLP-1 and GLP-2 ) and
glucose dependent insulinotropic peptide (
GIP ) . -
pubmed
GLP-1 and
GIP enhance
insulin secretion ;
GLP-2 increases intestinal growth and
glucose absorption . -
pubmed
T1R1 -
T1R3 combination co - expressed on the apical domain of cholecystokinin (
CCK ) expressing cells is a luminal sensor for a number of
L - amino acids ; with
amino acid - activation of the receptor eliciting
CCK secretion . -
pubmed
This article focuses on the role of the gut - expressed
T1R1 ,
T1R2 and
T1R3 in intestinal sweet and
L - amino acid sensing . -
pubmed
Assessment of differences in the conformational flexibility of
hepatitis B virus core - antigen and
e - antigen by
hydrogen deuterium exchange - mass spectrometry . -
pubmed
Here we use
hydrogen deuterium exchange - mass spectrometry (
HDX -
MS ) to study their structural properties . -
pubmed
Cp (- 10 ) 149d undergoes a drastic structural change when the intermolecular
disulphide bridge is reduced , adopting a
Cp149d -like structure , as evidenced by the detected HDX dynamics being more consistent with
Cp149d in many , albeit not all , regions . -
pubmed
Whilst
Fab E1 binds to
Cp149c and
Cp149d , it does not bind non - reduced and reduced
Cp (- 10 ) 149d , despite unhindered access to the epitope . -
pubmed
AIM : The author reported outcomes of a new simplified off - midline technique for closure of the defect after complete excision of the
sinus tracts . -
pubmed
The most important of these mechanisms is the presence of an inducible
erythromycin methylase (
erm ) gene , because it confers
macrolide resistance . -
pubmed
After that , blood samples were collected to determine blood viscosity , plasma viscosity , haematocrit ( HCT ) ,
fibrinogen (
FIB ) , blood lipid profile ( including total
cholesterol (
TC ) ,
low - density lipoprotein -
cholesterol (
LDL - C ) ,
triglyceride (
TG ) and
high - density lipoprotein -
cholesterol (
HDL - C ) ) ,
nitric oxide (
NO ) ,
6 - keto - prostaglandin ( PG ) F1alpha ,
endothelin (
ET ) and
thromboxane ( TX ) B2 . -
pubmed
The joint effects of YXC and swimming showed significant changes in the examined parameters : ( 1 ) decreases in plasma viscosity , blood viscosity and FIB ; ( 2 ) increases in
NO and
6 - keto - PGF1alpha ; ( 3 ) decreases in
ET and
TXB2 ; and ( 4 ) decreases in LDL - C and
TG . -
pubmed
Endovascular treatment consisted of
coil and
Onyx embolization for sacrifice of the parent vessel proximal to the lesion . -
pubmed
Ribosomal S6 kinase 1 (
S6K1 ) phosphorylation , a downstream target of
rapamycin (
mTOR)-phosphorylation kinase , increased following ECC +
BFR200 as well as ECC . -
pubmed
Anti - HA was mixed and interacted with
FAM - labeled HA tag ( FAM -
E4 ) inside the capillary . -
pubmed
The addition of excess
E4 or
TAMRA - E4 could lead to partial dissociation of the complex and take a two - step mechanism including dissociation and association . -
pubmed
In the last few weeks , the FDA approved three new therapies for
multiple myeloma :
ixazomib , the first oral proteasome inhibitor ; and
daratumumab and
elotuzumab , two monoclonal antibodies that target
CD38 and
SLAMF7 , respectively . -
pubmed
Design and synthesis of novel Y - shaped
barbituric acid derivatives as
PPARgamma activators . -
pubmed
Fourteen novel compounds were synthesized using
hydroxyl group protection - deprotection strategies for
PPARgamma activation . -
pubmed
BACKGROUND : New Delhi
metallo - beta - lactamase-1 (
NDM-1 ) is a relatively recent carbapenemase enzyme that inactivates all
beta - lactam antibiotics with the exception of
aztreonam . -
pubmed
METHODS : Over a period of one year , all Enterobacteriaceae isolates from all clinical specimens with reduced susceptibility to at least one
carbapenem and resistance to at least one third generation
cephalosporin were subjected to antibiotic susceptibility testing by disk diffusion and molecular detection of the
NDM-1 gene by single - target PCR followed by gel electrophoresis . -
pubmed
Our results demonstrated that pretreatment with
CGA ameliorated the intestinal
mitochondrial injury induced by
H2O2 ; membrane potential was increased , mitochondrial swelling , levels of reactive
oxygen species , contents of
8 - hydroxy-2 - deoxyguanosine , and
cytochrome c released were decreased . -
pubmed
The beneficial effects of
CGA were accompanied by an increase in antioxidant and
respiratory - chain complex I , IV , and V activities . -
pubmed
Fatty acid - binding proteins (
Fabp ) belong to a multigene family that are highly conserved in sequence and function , but differ in their gene regulation , suggesting selective pressure is exerted via regulatory elements in the promoter . -
pubmed
In vivo comparison of endometrial breakdown induced by
progesterone withdrawal to that seen during sustained
progesterone exposure , in the presence of
NF - kappaB inhibitors , revealed that
NF - kappaB - mediated functional
progesterone withdrawal is involved in endometrial breakdown in this implant model . -
pubmed
The hypervernalization response is achieved by increased enrichments of
VIN3 and trimethylation of
Histone H3 Lys 27 at the
FLC locus without invoking the increased enrichment of
Polycomb Repressive Complex 2 . -
pubmed
Correction :
Dasatinib , a
Src inhibitor , sensitizes
liver metastatic colorectal carcinoma to
oxaliplatin in
tumors with high levels of
phospho - Src . -
pubmed
SEM ,
TEM , EDX , FTIR , XRD , UV - vis and XPS analyses were employed to characterize the composition , structure , and morphology of
ZrO2 -
TiO2 hollow spheres . -
pubmed
A voltammetric sensor was designed to analyze
thiosulfate (
TS ) ,
4 - chlorophenol ( 4 -
CP ) and
nitrite (
NT ) , which are water pollutants , in mixed samples by combining the high conductive properties of
NiO nanoparticle (
NiO / NPs ) and the excellent mediator properties of novel
catechol derivative (
2,4 - dimethyl - N '-[ 1 -( 2,3 - dihydroxyphenyl ) methylidene ] aniline ) . -
pubmed
2,4 - Dimethyl - N '-[ 1 -( 2,3 - dihydroxyphenyl ) methylidene ] aniline was synthesized for the first time and proposed as a high - quality modifier for electrocatalytic analysis of
TS and its simultaneous analysis in the presence of
4 - CP and
NT . -
pubmed
In recent clinical trials , the \"quality\ " of
warfarin management has been characterized by the time in therapeutic range (
TTR ) -- with the therapeutic range being an INR between 2.0 and 3.0 . -
pubmed
Resolution of acute
inflammation is governed , in part , by lipid mediator class switching from proinflammatory
eicosanoids to specialized proresolving mediators , including a recently identified new pathway of mediators , termed
maresin conjugates in tissue regeneration ( MCTR ) , which includes
MCTR1 ,
MCTR2 , and
MCTR3 . -
pubmed
Here , we addressed whether each
MCTR can impact the known vascular actions of
cysteinyl leukotrienes . -
pubmed
Sirt5 + cells in wild - type
Kras CRCs are resistant to either chemotherapeutic agents or
cetuximab and serve as a reservoir for
recurrence . -
pubmed
Sirt5 demalonylates and inactivates
succinate dehydrogenase complex subunit A (
SDHA ) , leading to an accumulation of the oncometabolite
succinate . -
pubmed
Succinate binds to and activates a reactive
oxygen species - scavenging enzyme ,
thioredoxin reductase 2 (
TrxR2 ) , to confer chemotherapy resistance . -
pubmed
In contrast ,
Sirt5 + cells exhibit an elevated
succinate - to -
aKG ratio that inhibits
aKG - dependent
dioxygenases to maintain
cetuximab resistance . -
pubmed
CCK - receptor transcripts were also detected and
Ca2 + imaging showed that
CCK stimulation increased [
Ca2 +]cyt in a dose - responsive manner that was sensitive to CRAC - channel inhibitors . -
pubmed
These results provide the first evidence of a potential regulatory system for SOCE in enamel cells and so strengthen the
Ca2 + transcytosis paradigm for
ER - based transport of bulk
Ca2 + . -
pubmed
Our findings also implicate enamel cells as a new physiological target of
CCK and raise the possibility of an auto / paracrine system for regulating
Ca2 + transport . -
pubmed
Ca2 + - sensing receptor (
CaSR ) represents a potential therapeutic target for
inflammatory bowel diseases and strongly prefers
aromatic amino acid ligands . -
pubmed
We investigated the regulatory effects of dietary supplementation with
aromatic amino acids -
tryptophan ,
phenylalanine and
tyrosine (
TPT ) - on the
CaSR signalling pathway and intestinal inflammatory response . -
pubmed
Dietary supplementation of
TPT alleviated
histopathological injury and decreased
myeloperoxidase activity in intestine challenged with lipopolysaccharide . -
pubmed
Supplementation of
TPT increased
CaSR and
phospholipase Cbeta2 protein levels , but decreased inhibitor of
NF - kappaB kinase alpha / beta and
inhibitor of NF - kappaB (
IkappaB ) protein levels in the lipopolysaccharide - challenged piglets . -
pubmed
To conclude , supplementation of
aromatic amino acids alleviated intestinal
inflammation as mediated through the
CaSR signalling pathway . -
pubmed
This microfluidic bioanalysis platform was used in conjunction with commercial " mix - detect " assays for
glucose , total
bile acids , and
lactate dehydrogenase (
LDH ) . -
pubmed
To highlight this application , hepatocyte spheroids were established in microfluidic devices , injured on - chip by exposure to lipotoxic agent (
palmitate ) , and then connected to the bioanalysis module for daily monitoring of changes in
cytotoxicity (
LDH ) , energy metabolism (
glucose ) , and liver function ( total
bile acids ) . -
pubmed
Microfluidic in - droplet assays revealed increased levels of
LDH as well as reduction in
bile acid synthesis - results that were consistent with
hepatic injury . -
pubmed
We have developed a clustering of
kinase conformations based on the location of the
Phe side chain ( DFGin , DFGout , and DFGinter or intermediate ) and the backbone dihedral angles of the sequence X - D - F , where X is the residue before the DFGmotif , and the DFG -
Phe side - chain rotamer , utilizing a density - based clustering algorithm . -
pubmed
The
beta - propeller phytase (
BPP ) is an enzyme that hydrolyzes phyate to release inorganic
phosphorus . -
pubmed
Banked bone flaps had the highest overall complication rates ( 51 percent ; p & lt ; 0.001 ) ,
bone resorption ( 39.7 percent ; p & lt ; 0.001 ) , and failure rates ( 40.2 percent ; p & lt ; 0.001 ) , whereas
polyetherether ketone had the highest rates of
infection (
16.1 percent ; p & lt ; 0.001 ) . -
pubmed
METHODS : Specimens ( N = 288 ; n = 96 per material ) with a thickness of 1 + - 0.03 mm of the following
CAD / CAM materials were fabricated : resin composite ( Lava Ultimate , 3 M ) , polymer - infiltrated ceramic ( VITA Enamic , VITA Zahnfabrik ) , and
leucite ceramic (
IPS Empress
CAD , Ivoclar Vivadent ) . -
pubmed
Resin composite Lava Ultimate showed larger E values compared with the resin hybrid ceramic VITA Enamic and
leucite ceramic
IPS Empress
CAD . -
pubmed
Editor 's Note :
PrLZ Protects
Prostate Cancer Cells from Apoptosis Induced by
Androgen Deprivation via the Activation of
Stat3 /
Bcl-2 Pathway . -
pubmed
Hormonal assessment included total
testosterone ( TT ) ,
sex hormone binding globulin (
SHBG ) , free
androgen index ( FAI ) , Anti
Mullerian Hormone (
AMH ) ,
17 - hydroxyprogesterone (
17 - OHP ) ,
dehydroepiandrosterone sulfate (
DHEAS ) ,
follicle stimulating hormone (
FSH ) ,
luteinizing hormone (
LH ) ,
thyroid stimulating hormone (
TSH ) and
prolactin . -
pubmed
VIP and muscarinic synergistic mucin secretion by salivary mucous cells is mediated by enhanced
PKC activity via
VIP - induced release of an intracellular
Ca2 + pool . -
pubmed
Mucin secretion by salivary mucous glands is mediated predominantly by parasympathetic
acetylcholine activation of cholinergic
muscarinic receptors via increased intracellular free
calcium ( [
Ca2+]i ) and activation of conventional
protein kinase C isozymes (
cPKC ) . -
pubmed
VIP - induced secretion is mediated by
cAMP - activated
protein kinase A (
PKA ) , independently of increased [
Ca2+]i . -
pubmed
Synergistic secretion between
VIP and the muscarinic agonist ,
carbachol , was demonstrated but only with submaximal
carbachol . -
pubmed
Instead ,
PKA activated by
VIP releases
Ca2 + from an intracellular pool maintained by the
sarco / endoplasmic reticulum Ca2 + - ATPase pump . -
pubmed
Calcium release was independent of
phospholipase C activity . -
pubmed
Synergistic mucin secretion was mimicked by
VIP plus either
phorbol 12 - myristate 13 - acetate or 0.01 muM
thapsigargin , and blocked by the
PKC inhibitor ,
Go6976 . -
pubmed
Synergism is therefore driven by
VIP - mediated [
Ca2 +]i augmenting
cPKC activity to enhance
muscarinic mucin secretion . -
pubmed
Additional data suggest
ryanodine receptors control
VIP /
PKA - mediated
Ca2 + release from a
Ca2 + pool also responsive to maximal
carbachol . -
pubmed
One such etiology found in pregnancy is
HL , in which high levels of
beta - human chorionic gonadotropin can stimulate production of benign theca
lutein cysts , leading to marked
hyperandrogenism and
virilizing symptoms . -
pubmed
Ulinastatin , a
urine trypsin inhibitor , relieves
systemic inflammation and improves coagulation profiles with however sparse evidence of its effects on
blood loss and allogeneic transfusion in this specific population . -
pubmed
RESULTS : Compared with placebo ,
ulinastatin significantly reduced the volume of
post - operative blood loss within 24 h ( 688.39 + - 393.55 ml vs 854.33 + - 434.03 ml MD - 165.95 ml , 95%CI - 262.88 ml to - 69.01 ml , p < 0.001 ) and the volume of allogeneic erythrocyte transfusion ( 2.57 + - 3.15 unit vs 3.73 + - 4.21 unit , MD-1.16 unit , 95%CI - 2.06 units to - 0.26 units , p = 0.002 ) . -
pubmed
CONCLUSIONS :
Ulinastatin reduced
post - operative blood loss and allogeneic erythrocyte transfusion in heart surgery with
CPB . -
pubmed
Bioreducible
Phosphonoamidate Pro - drug Inhibitor of
Enolase : Proof of Concept Study . -
pubmed
We recently described a promising
phosphonate inhibitor of
enolase for
cancers harboring homozygous deletions of
ENO1 . -
pubmed
We demonstrated that this mutation caused impairment of mitochondrial translation ,
respiratory deficiency , overproduction of reactive
oxygen species ( ROS ) , and decreased mitochondrial membrane potential (
MMP ) , which ultimately led to severe
mitochondrial myopathy . -
pubmed
Interactions between
carbohydrates ( glycans ) and glycan - binding proteins (
GBPs ) regulate a wide variety of important biological processes . -
pubmed
The general suppression of nonspecific adducts ( including nonvolatile buffers and salts ) achieved with these tips enables ESI -
MS glycan affinity measurements to be performed on
C - type lectins , a class of
GBPs that bind glycans in a
calcium - dependent manner and are important regulators of immune response . -
pubmed
What is the best method to remove
2,3 - diphosphoglycerate from
hemoglobin ? -
pubmed
The
gamma - carboxyglutamic acid and
epidermal growth factor - like domains of
factor X. -
pubmed
We have isolated fragments , from tryptic digests of
factor X , that consists of the
gamma - carboxyglutamic acid (
Gla ) region linked to one or two
epidermal growth factor (
EGF)-like domains . -
pubmed
The same conclusion was reached when
thrombin generation was studied in a system of purified components (
factor Xa ,
factor Va ,
prothrombin , phospholipid , and
Ca2 + ) . -
pubmed
However , experiments in the purified system without phospholipid indicated a direct , albeit weak , interaction between the Gla region of
factor Xa and
factor Va and between the
COOH - terminal
EGF - like domain of
factor Xa and
factor Va. -
pubmed
Using domain - specific Fab fragments , we have confirmed that the conformation of the
serine protease region alters dramatically upon activation of
factor X. -
pubmed
Using
T7 gene 6 exonuclease , the 3'termini of the fragments were exposed and then hybridized in
solution to a biotinylated oligonucleotide complementary to one end of the SUEHGR fragment . -
pubmed
Tamoxifen and
trifluoroperazine (
Stelazine ) potentiate cytostatic /
cytotoxic effects of
P-30 protein , a novel protein possessing anti -
tumor activity . -
pubmed
This
reproductive sterility has been attributed to
chlordecone 's apparent " estrogenicity " and its ability to inhibit the release of
luteinizing hormone (
LH ) from the anterior pituitary . -
pubmed
Chlordecone ( 25 - 75 mg / kg ) administration at the time of
PMS exposure did not inhibit the ovulatory response to
HCG . -
pubmed
The specific effect of
chloroquine in ' stripping
HLA ' from RBC had been assumed to support the concept that
HLA class I was adsorbed from plasma . -
pubmed
We suggest that the action of
chloroquine is to remove
beta 2 m alone , which prevents normal class I expression and also results in conformational changes to the
class I heavy chain , but that it is not capable of removing the membrane - bound heavy chain . -
pubmed
Serum levels of
3 - keto - desogestrel and
SHBG during 12 cycles of treatment with 30 micrograms
ethinylestradiol and 150 micrograms
desogestrel . -
pubmed
There was a significant correlation between the
SHBG levels and the area under the KDG - concentration - versus - time curves ( AUC ) indicating a pronounced influence of the
serum steroid - binding protein upon the pharmacokinetics of
KDG . -
pubmed
This study , which was carried out as part of an in vitro fertilisation programme (
IVF ) was performed during 162 cycles of which 22 were spontaneous and 140 were stimulated by
Clomiphene and
HMG . -
pubmed
There is a correlation between the levels of
progesterone in the blood and the thickness of the endometrium on the 2nd
post - laparoscopy day . -
pubmed
Ketoconazole , a known
cytochrome P-450 inhibitor , inhibited both
progesterone ring hydroxylation and side - chain oxidation to
steroidal acids . -
pubmed
These findings strongly suggest that
AM blocks slow inward current inflow via
Ca2 + channels in the ventricular muscle . -
pubmed
Serum
osteocalcin was elevated , but there was little effect on serum
alkaline phosphatase ,
Ca , P , or
calcitonin . -
pubmed
Pretreatment of animals with
5 - azacytidine results in a different response of
tryptophan oxygenase to
tryptophan and
cortisone administration . -
pubmed
Tryptophan affects also the level of
thymidine kinase at various stages of liver regeneration . -
pubmed
5 - azacytidine given 16 - 40 hours prior to partial hepatectomy resulted in the enhancement of
thymidine kinase activity at 24 hours of regeneration . -
pubmed
ACTH - secreting medullary
carcinoma of the thyroid : monitoring of clinical course with
calcitonin and
cortisol assays and immunohistochemical studies . -
pubmed
This
tumor inhibitory factor co - purified with
transforming growth factor activity through acid /
ethanol extraction and Bio - Gel chromatography . -
pubmed
The
tumor inhibitory factor is heat stable and requires
disulfide bonds for bioactivity . -
pubmed
The heat - stable polypeptide (
APF-1 ) required for
ATP - dependent proteolysis in reticulocytes enters into high molecular weight conjugates upon incubation with the fraction of reticulocytes that is retained by
DEAE - cellulose . -
pubmed
The
APF-1 conjugates are stable in
sodium dodecyl sulfate /
polyacrylamide gel electrophoresis and Sephadex G-75 isolation and are resistant to mild acid , alkali , heat denaturation , and reduction ; the conjugates are therefore covalent . -
pubmed
1 The effects of pretreatment with various inhibitors of anaphylactic mediators on antigen - induced bronchoconstriction were studied in anaesthetized guinea - pigs , actively sensitized according to two different regimens ( one producing
IgE- and
IgG - like antibodies and the other producing exclusively
IgG antibodies).2 The
phospholipase A(2)-inhibitors
mepacrine and
p - bromphenacylbromide caused a dose - dependent inhibition of the antigen - induced bronchoconstriction in guinea - pigs sensitized to produce both
IgE and
IgG antibodies . -
pubmed
In this model , the residual bronchoconstriction evident after combined pretreatment with
indomethacin and
mepyramine was prevented by additional pretreatment with
mepacrine ,
FPL 55712 or
budesonide.5 Arachidonic acid given intravenously caused a marked
bronchoconstriction that was prevented by
indomethacin but not by
budesonide ,
FPL 55712 or
p - bromphenacylbromide.6 Although the same pattern of anaphylactic mediators is released in the two models of
anaphylactic bronchoconstriction , a different activation mechanism is indicated by the results obtained with
phospholipase A ( 2 ) inhibitors . -
pubmed
This contrasts with the primary action of
gamma - vinyl - GABA on
GABA - transaminase activity . -
pubmed
Oxazepam is determined at 254 nm by using a
C18 column . -
pubmed
In the presence of
FBS ,
calcein leakage was increased with
PEG - Chol percentage incorporation and an increase in
FBS concentration . -
pubmed
Exposure of mesangial cells to
platelet - derived growth factor ( PDGF ) BB caused a significant stimulation of cell proliferation and protein synthesis , as measured by
[ 3H ] thymidine incorporation and
[ 3H ] leucine incorporation respectively . -
pubmed
Treatment of mesangial cells with
PDGF and
angiotensin II rapidly and dose - dependently stimulated
mitogen - activated protein ( MAP ) kinase activity , as shown by an assay for activity in vitro using
myelin basic protein as a substrate , and by immunoprecipitation of
32P - labelled cells with specific antibodies against the 42 kDa and 44 kDa
mitogen - activated protein kinases p42mapk and
p44mapk , respectively . -
pubmed
Whereas stimulation with
PDGF - BB caused a potent and sustained ( for more than 30 min ) phosphorylation and activation of
p42mapk and
p44mapk , as well as of the upstream activators
MAP kinase kinase and
c - Raf , the effect of
angiotensin II was less potent , reaching a peak at 5 - 10 min and thereafter declining rapidly . -
pubmed
In summary , these results suggest that
PDGF - BB and
angiotensin II differ in their potency and duration of activation of the
MAP kinase cascade , which may explain why
PDGF - BB is a potent mitogen for mesangial cells , whereas
angiotensin II only triggers mesangial - cell
hypertrophy . -
pubmed
A sustained increase ( P & lt ; 0.05 ) in
stroke volume accompanied the prompt and sustained decrease in
plasma renin activity and sustained increase ( P & lt ; 0.05 ) in plasma
atrial natriuretic peptide during
Eu - H2O and
Hypo - H2O. -
pubmed
We feel that there is now compelling evidence that the
GTP - binding proteins play more than just a coordinating role in the actions of both
tyrosine kinase and
nontyrosine kinase receptor signal transduction . -
pubmed
Tumour markers in
uraemia :
carbohydrate antigen
CA 72 - 4 ,
carcinoembryonic antigen and
carbohydrate antigen
CA 19 - 9 . -
pubmed
Thus , the
retinoic acid response mediated by
RAR alpha is weak in these cells . -
pubmed
Glutamate responses and spontaneous excitatory
post - synaptic potentials in DE2 were reversibly blocked in
sodium - free saline . -
pubmed
Steady - state potentials reached during
glutamate superfusion were similar to the reversal potentials for both the spontaneous
post - synaptic potentials and
glutamate , also suggesting that these potentials may be glutamatergic . -
pubmed
Non - N - methyl - D - aspartate receptor antagonists partially blocked spontaneous DE2 excitatory potentials and responses elicited by exogenous
glutamate and
kainate . -
pubmed
The thermal antinociceptive effect induced by
morphine administered in the periaqueductal gray was significantly attenuated by
neuropeptide FF , whereas that induced by systemically administered
morphine was not . -
pubmed
The interaction of
neuropeptide FF with intracerebrally or systemically administered
morphine in a test of
tactile allodynia was not significant . -
pubmed
This antiallodynic effect is at least partly independent of
naloxone - sensitive
opioid receptors . -
pubmed
Furthermore ,
neuropeptide FF in the periaqueductal gray attenuates antinociception induced by intracerebrally but not systemically administered
morphine . -
pubmed
Endocrinological contributions to oncology pertain in particular to the discovery of active substances which inhibit neovascularization (
endostatins ) and some new aspects of the action of antioestrogens and
oestrogens . -
pubmed
The possible action of hormonal factors in neurodegenerative processes are obvious in
ubiquitine ,
CREB and " heat
shock proteins " . -
pubmed
Subsequently attention is paid to
ryanodine receptors and compounds acting as
Ca on the sensor for
Ca in the parathyroid bodies and inhibition parathormone secretion . -
pubmed
The purpose of this study was to demonstrate the immunological effects on the
IgG - antibody repertoire in
EAU after topical and systemic
allopurinol and
steroids . -
pubmed
Morphological changes of the small bowel mucosa and activities of daily life (
ADL ) after
PEG were also investigated . -
pubmed
RESULTS : In the TPN - to -
PEG group , the serum level of
total protein and
albumin , length of duodenal villi , ration of positive proliferative cells , width of duodenal microvilli and
ADL markedly increased . -
pubmed
In the NG - to -
PEG group ,
body weight and
ADL were markedly improved after
PEG . -
pubmed
Calcium influx through
voltage - dependent calcium channels ( VDCCs ) mediates a variety of functions in neurons and other excitable cells , but excessive
calcium influx through these channels can contribute to neuronal
death in pathological settings . -
pubmed
Prolonged exposure to
4HN results in neuronal
death , which is prevented by treatment with
glutathione and attenuated by the
L - type Ca2 + channel blocker
nimodipine . -
pubmed
Tyrosine phosphorylation of alpha1 VDCC subunits is increased in neurons exposed to
4HN , and studies using inhibitors of
tyrosine kinases and phosphatases indicate a requirement for
tyrosine phosphorylation in the enhancement of VDCC activity in response to
4HN . -
pubmed
At
rest , the
phosphorus -
creatinine indexes were similar in all groups ; pH values at
rest did not vary either . -
pubmed
Expression of
Sox9 in granulosa cells lacking the
estrogen receptors ,
ERalpha and
ERbeta . -
pubmed
These findings suggest that functional Sertoli cells can transdifferentiate from mature granulosa cells devoid of
estrogen receptors as a result of
Sox9 expression . -
pubmed
Bioassay of
Fluometuron for Possible Carcinogenicity (
CAS No . -
pubmed
The effect of
carotene on the
lactic acid production and
hexokinase activity of hemolysates from
cancerous and noncancerous persons . -
pubmed
Both
Ero1 -
Lalpha and
Ero1 - Lbeta interact via
disulfide bonds with
PDI and support the oxidation of immunoglobulin light chains . -
pubmed
In contrast , extracellular
HbO2 is highly vasoconstrictive , and the resultant
hypertension is a significant side effect of most
hemoglobin - based blood substitutes . -
pubmed
This interpretation is supported by a strong linear correlation between the magnitude of the blood pressure effect caused by infusion of cross - linked recombinant
hemoglobin tetramers in vivo and the rate of
NO dioxygenation by these proteins measured in vitro . -
pubmed
Calreticulin , a
Ca ( 2 + ) chaperone , is found in many different locations in various eukaryotic cells , including lumen of the endoplasmic reticulum , the cell surface , perinuclear areas and cytosolic granules . -
pubmed
In parallel with these changes , exendin-4 decreased the
cytokine - induced activation of
caspase-3 ( by 46 % , p<0.05 ) , and decreased levels of
inducible nitric oxide synthase ( by 71 % , p<0.05 ) and reactive
oxygen species ( by 27 % , p<0.05 ) . -
pubmed
Recently , we proved that
tobacco CND41 protease is involved in
Rubisco degradation and the translocation of
nitrogen during senescence . -
pubmed
Using very specific antibodies that were prepared against
CND41 - specific peptide ( anti - Val 186 to
Ser 206 ) , immunoblot analysis clearly indicated a change in the accumulation and processing of
CND41 during the maturation of leaves in whole plants . -
pubmed
Further studies of seedlings under senescence induced by combined treatment with
nitrogen -
starvation and high
sucrose confirmed that the processing of
CND41 was important for
protease activity and senescence . -
pubmed
Use of
ACTH and
prednisolone in
infantile spasms : experience from a developing country . -
pubmed
BACKGROUND :
Adrenocorticotrophic hormone (
ACTH ) and
prednisone are both used to treat
infantile spasms (
IS ) in
West syndrome . -
pubmed
ACTH was expensive and difficult to obtain whereas
prednisolone was cheap and easily available . -
pubmed
The cost of
ACTH injection was more than 100 times the cost of oral
prednisolone . -
pubmed
Although the protein
GlmD(
Tk ) was predicted as a probable
sugar isomerase related to the C - terminal
sugar isomerase domain of
GlcN6P synthase , the recombinant
GlmD(
Tk ) clearly exhibited
GlcN6P deaminase activity , generating
Fru6P and
ammonia from
GlcN6P. -
pubmed
This enzyme also catalyzed the reverse reaction , the
ammonia - dependent amination / isomerization of
Fru6P to
GlcN6P , whereas no
GlcN6P synthase activity dependent on
glutamine was observed . -
pubmed
kodakaraensis cells ,
glmD(
Tk ) was polycistronically transcribed together with upstream genes encoding an
ABC transporter and a downstream
exo - beta - glucosaminidase gene (
glmA(
Tk ) ) within the gene cluster , and their expression was induced by the chitin degradation intermediate ,
diacetylchitobiose . -
pubmed
The results presented here indicate that
GlmD(Tk ) is actually a
GlcN6P deaminase functioning in the entry of chitin - derived
monosaccharides to glycolysis in this hyperthermophile . -
pubmed
A comparative study was carried out for the simultaneous determination of 11
pyrethroid insecticides in soil by gas chromatography ( GC)--ion trap ( IT)--mass spectrometry (
MS ) , by means of two different ionization modes ; electron impact and negative chemical ionization and three data acquisition procedures ; full scan , selected ion monitoring and
MS /
MS . -
pubmed
The mutual diffusion coefficients ( D ) of monomeric
actin were accurately determined in a G - buffer with a low concentration of
KCl from 0 to 10 mM. -
pubmed
The Hamaker constant of
actin in the
Ca ( 2 +)- ATP bound state was determined for the first time as A(H ) = 10.4 + /- 0.6 k(B)T. -
pubmed
Tissue total antioxidant capacity ( T - AOC ) ,
superoxide dismutase (
SOD ) , and
thiobarbituric acid reaction substance ( TBARS ) were determined on the 2nd , 4th , and 6th day . -
pubmed
BACKGROUND : Intermittent dosing of
calcitriol for
secondary hyperparathyroidism (
SHPT ) has been associated with greater
parathyroid hormone (
PTH ) reduction with fewer calcemic and phosphatemic effects than daily ( QD ) dosing . -
pubmed
These cells had an increased sensitivity to IGF-1 and to
cyclic AMP , which required
phosphatidylinositol-3 ( PI3 )- kinase and the
mitogen - activated protein kinase ,
extracellular signal - regulated protein kinase (
ERK ) for enhanced growth . -
pubmed
METHODS : In the present study , the association between
ARHI and polymorphisms in genes that contribute to the defence against reactive
oxygen species , including
GSTT1 ,
GSTM1 and
NAT2 , was tested . -
pubmed
Such action is mediated by interaction of
androgen receptor (
AR ) promoter with
nuclear proteins , which are involved in transcriptional regulation of
androgen responsive genes . -
pubmed
The
cyclin - dependent kinase inhibitor
p27 ( Kip1 ) arrests cell cycle progression through
G1 / S phases and is regulated by phosphorylation of
serine /
threonine residues . -
pubmed
Recently , we identified the
serine / threonine kinase ,
KIS , which phosphorylates
p27 ( Kip1 ) on
serine 10 leading to nuclear export of
p27 ( Kip1 ) and protein degradation . -
pubmed
Signaling by the peptide ligand
apelin and its cognate
G protein - coupled receptor APJ has a potent inotropic effect on cardiac contractility and modulates systemic vascular resistance through
nitric oxide - dependent signaling . -
pubmed
In addition , there is evidence for counterregulation of the
angiotensin and
vasopressin pathways . -
pubmed
The QSAR is an alternative method for the research of new and better
Vitamin D analogues with affinity for the
VDR receptor . -
pubmed
This paper describes the results of applying the Radial Distribution Function ( RDF descriptors ) approach for predicting the
VDR affinity of 38
vitamin D analogues . -
pubmed
CONCLUSION : Repeated determination of the BASDAI and BASFI by the patients ( using paper forms or personal digital assessments ) may help to identify candidates for treatment intensification , to optimize
infliximab injection regimens in good responders , and to avoid unnecessary switching from one
TNFalpha antagonist to another . -
pubmed
Theoretical studies on the thermochemistry of stable closed - shell
C1 and
C2 brominated hydrocarbons . -
pubmed
The enthalpies of formation of stable closed shell
C1 and
C2 brominated
hydrocarbons have been predicted using Gaussian-3X model chemistry . -
pubmed
124 , 064106 ( 2006 ) ] , which requires only a small number of fast nonequilibrium MD simulations of the system in both forward and time reversed directions along the RC , we reconstruct the
PMF : ( 1 ) of
deca - alanine as a function of its end - to - end distance , and ( 2 ) that guides the motion of
potassium ions through the
gramicidin A channel . -
pubmed
Serine endopeptidases ,
cutinases ,
acetylcholinesterases ,
cellulases , among other enzymes , contain these
triads . -
pubmed
The optimisation of the most important operating conditions affecting
lipase - catalysed transesterification and the yield of
alkyl esters , such as the type and form of
lipase , the type of
alcohol , the presence of organic solvents , the content of water in the oil , temperature and the presence of
glycerol , are discussed . -
pubmed
hERG1 is a member of the
cyclic nucleotide binding domain family of
K (+ ) channels . -
pubmed
Alignment of
cyclic nucleotide binding domain channels revealed an evolutionary conserved sequence HwX(A / G)C in the
S5 domain . -
pubmed
We reasoned that
histidine 562 in
hERG1 could play an important structure - function role . -
pubmed
To explore this role , we created in
silica models of the
hERG1 pore domain based on the
KvAP crystal structure with Rosetta - membrane modeling and molecular - dynamics simulations . -
pubmed
Simulations indicate that the H562 residue in the
S5 helix spans the gap between the
S5 helix and the pore helix , stabilizing the pore domain , and that mutation at the H562 residue leads to a disruption of the
hydrogen bonding to T618 and S621 , resulting in distortion of the selectivity filter . -
pubmed
In conclusion , interactions between H562 in the
S5 helix and
amino acids in the pore helix are important determinants of
hERG1 potassium channel function , as confirmed by theory and experiment . -
pubmed
Other PFCs such as
perfluorohexanesulfonate (
PFHxS ) ,
perfluorooctanoic acid (
PFOA ) , and acids with chain lengths up to
C11 were also detected , with mean levels ranging between 0.50 and 1.45 ng / g wet weight . -
pubmed
Accumulation in liver is suggested for
PFOS and the
perfluorocarboxylic acids with
carbon chain lengths
C9 ,
C10 , and
C11 . -
pubmed
Newer promising strategies along with recent patented inventions may also include
thiazolidinediones ,
folates ,
tetrahydrobiopterin ,
cyclopentone prostaglandins and
aldose reductase inhibitors with well known effects on vascular redox , but with still unclear results on clinical outcome . -
pubmed
In experimentally exploring the adaptive landscape of the evolution of resistance to
beta - lactam antibiotics in enteric bacteria , we noted that a regulatory mutation that increases
beta - lactamase expression by about 2 - fold has a very strong tendency to be fixed at or near the end of the evolutionary pathway . -
pubmed
To understand the basis of the difference , we carried out experiments in which the expression of
beta - lactamase was under the control of a tunable
arabinose promoter . -
pubmed
Best medical treatment is defined as optimal drug therapy for control of the major risk factors ( blood pressure & lt ; or = 125/80 mmHg ,
LDL cholesterol & lt ; or = 100 mg / dL , HbA1c & lt ; or = 6.5 % ) . -
pubmed
A theoretical study on binding orientations and quantitative structure - activity relationship of
thiophene derivatives as inhibitors towards
tubulin has been carried out by using the docking analysis and the comparative molecular field analysis . -
pubmed
The conjugation reaction was effected by
squaric acid chemistry and monitored in virtually real time by SELDI -
TOF MS . -
pubmed
In samples stimulated with 0.3 microm
ADP ,
fibrinogen binding decreased from 41 + /- 4 % to 31 + /- 3 % ( P & lt ; 0.01 , n = 11 ) after
acetylcholine administration . -
pubmed
The vasodilator response to
acetylcholine was significantly impaired during infusions of
ET-1 and
l - NMMA . -
pubmed
ET-1 did not affect platelet activity per se , whereas
l - NMMA increased platelet
P - selectin expression . -
pubmed
Both
ET-1 and
l - NMMA attenuated the
acetylcholine - induced inhibition of platelet activity . -
pubmed
CONCLUSIONS : Our study indicates that , further to inhibiting endothelium - dependent vasodilatation ,
ET-1 may also attenuate endothelium - dependent inhibition of platelet activation induced by
acetylcholine . -
pubmed
The novel roles of
glucagon - like peptide-1 ,
angiotensin II , and
vitamin D in islet function . -
pubmed
As such , the islets perform critical biological activities in synthesizing metabolic peptide hormones , notably
insulin and regulating body
glucose homeostasis . -
pubmed
Of great interest in this context ,
glucagon - like peptide-1 can improve glycemic control by regulating
insulin secretion and islet cell mass ;
vitamin D can regulate islet physiology directly by binding its receptors ; in addition , the peptide hormone
angiotensin II has been implicated in islet function and exhibits effects on islet cell secretion as well as cell mass . -
pubmed
Microbial respiration was positively correlated with the degradation rates of
metalaxyl ,
azoxystrobin and
chlorpyrifos , compared to
phenoloxidase which showed no correlation . -
pubmed
Pazopanib is an orally administered , multi -
tyrosine kinase inhibitor that interrupts
tumor growth in
renal cell carcinoma . -
pubmed
We directly compared predominant viral sequences derived from 52 mother - child transmission pairs following
vertical infection and identified 1,475 sites of mother - infant
amino acid divergence within
Nef ,
Gag , and
Pol . -
pubmed
Clearances of small solutes like
urea are higher than in hemofiltration and of middle solutes like
beta ( 2 )- microglobulin are higher than in hf -
HD . -
pubmed
Glucose and
fatty acids synergize to promote B - cell apoptosis through activation of
glycogen synthase kinase 3beta independent of
JNK activation . -
pubmed
Jun NH2 - terminal kinase (
JNK ) activation peaked at the lowest
glucose concentration , in contrast to a progressive reduction in
IRS2 protein and impairment of
insulin receptor substrate signaling . -
pubmed
CONCLUSIONS / SIGNIFICANCE :
Glucose in the presence of
FFA results in synergistic effects on
ER stress , impaired
insulin receptor substrate signaling and
Gsk3beta activation . -
pubmed
The first NMR structure of a
Cu ( I )-bound
metallochaperone model with the conserved sequence MT / HCXXC revealed that at pH ~3.0 and ~6.8
Cu ( I ) binds through one
Cys and the
Met rather than the two
Cys residues , differently than at pH ~8.5 . -
pubmed
Many of them rely on the
Hsp90 chaperone system for full function and are further fine - tuned by
Hsp90 - associated peptidyl -
prolyl isomerases such as
FK506 - binding proteins 51 and 52 . -
pubmed
The peptidyl -
prolyl isomerase domain of
FKBP51 contains a high - affinity binding site for the natural products
FK506 and
rapamycin and has further been shown to convey most of the inhibitory activity on the
glucocorticoid receptor . -
pubmed
Here , a series of high - resolution structures of the
peptidyl - prolyl isomerase domain of
FKBP51 as well as a cocrystal structure with the prototypic ligand
FK506 are described . -
pubmed
The results obtained using the computational methods gave valuable information about the probable metabolites of ( 1 ) ( qualitatively ) and also about the important interactions of this lead compound with the
amino acid residues of the active site of
CYP2C9 . -
pubmed
RESULTS : Of the 10 AVS criteria identified in the literature , one criterion (
ACTH stimulation , positioning :
cortisol [ adrenal]/
cortisol [ periphery ] [
Ca /
Cp ] & gt ; 5.0 and lateralization :
aldosterone /
cortisol [ A / C ] [ dominant { D } ] : A / C [ nondominant { ND } ] & gt ; 4:1 ) was the most accurate in identifying and correctly predicting lateralization of disease ( P value range : & lt ; 0.001 - 0.0369 ) . -
pubmed
The second most accurate criterion was no
ACTH stimulation , positioning
Ca / Cp & gt ; 1.1 and lateralization : A / C ( D ): A / C ( ND ) & gt ; 2:1 . -
pubmed
The
Ca ( 2 + ) /
calmodulin (
CaM ) signaling pathway mediates the heat stress ( HS ) response and acquisition of thermotolerance in plants . -
pubmed
Ca ( 2 + ) and
A23187 treatments under HS generated an intense and sustained increase in [
Ca ( 2 + ) ] cyt and accelerated the expression of
CaM1 - 1 and
sHSPC / N genes , which suggests that HS - induced apoplastic
Ca ( 2 + ) influx was responsible for the [
Ca ( 2 + ) ] cyt transient and downstream HS signaling . -
pubmed
The chromatographic separation was carried out on a Hypersil BDS
C18 column , and gradient elution was employed with a mobile phase containing
acetonitrile and water . -
pubmed
CYP1A1 and
CYP1B1 - mediated biotransformation of the antitrypanosomal
methamidoxime prodrug
DB844 forms novel metabolites through intramolecular rearrangement . -
pubmed
In the current study , we compared the metabolism of
DB844 by hepatic and extrahepatic
cytochrome P450s to determine whether differences in metabolite formation underlie the observed GI
toxicity . -
pubmed
In addition , MY was confirmed with a synthetic standard and detection of
nitric oxide (
NO ) release when
DB844 was incubated with
CYP1A1 . -
pubmed
dabigatran ) and
factor Xa inhibitors ( e.g. -
pubmed
In contrast , treadmill exercise and
MPH alleviated the
ADHD - induced the decrease of balance and the number of calbindine - positive cells , and the increase of
GFAP expression and
Bax /
Bcl-2 ratio in the cerebellum ( P< ; 0.05 , respectively ) . -
pubmed
Androgens ,
17 - beta - estradiol ( 17 beta -E2 ) , and
insulin levels were measured in plasma and/or serum and
leptin and
visfatin levels were assayed in both serum and follicular fluid ( FF -
leptin , FF -
visfatin ) . -
pubmed
Previously , we have shown that intermittent
parathyroid hormone (
PTH ) bone anabolic therapy involves
SOST expression reduction by inhibiting
myocyte enhancer factor 2 (
MEF2 ) , which activates a distant bone enhancer . -
pubmed
PTH - induced
Sost suppression was associated with specific rapid nuclear accumulation of
HDAC5 and co - localization with
MEF2s in nuclear speckles requiring
serine residues 259 and 498 , whose phosphorylations control nucleocytoplasmic shuttling . -
pubmed
Curcumin protects renal tubular epithelial cells from high
glucose - induced epithelial - to - mesenchymal transition through
Nrf2 - mediated upregulation of
heme oxygenase-1 . -
pubmed
This process may occur through abrogation of high
glucose ( HG)-induced oxidative stress via activation of
nuclear factor ( erythroid - derived 2 )- like 2 (
Nrf2 ) and
heme oxygenase-1 (
HO-1 ) in kidney tubular epithelial cells . -
pubmed
The present study revealed that
curcumin was able to prevent events associated with
EMT , including the downregulation of
E - cadherin and the increased expression of
alpha - smooth muscle actin . -
pubmed
Notably , knockdown of
Nrf2 with small interfering RNA prevented the
curcumin - induced elevation in expression of
HO-1 and the associated anti - fibrotic effects . -
pubmed
Clinical Research on
Albumin - Bound
Paclitaxel - Based Chemotherapy for Advanced
Esophageal Cancer . -
pubmed
Both
PBF and PAbdF were associated with
HDL and
LDL , but have opposite correlation with
triglyceride . -
pubmed
Men with GOb had lower levels of percent lean mass ( PLM ) ,
testosterone and bioavailable
testosterone , and higher
insulin and
glucose levels . -
pubmed
Men with AbO had lower arm and leg fat , higher
insulin levels and
triglycerides . -
pubmed
RESULTS :
KfoC has broad donor substrate specificity and acceptor promiscuity , making it an attractive tool enzyme for use in structurally - defined chimeric glycosaminoglycan
oligosaccharide synthesis in vitro . -
pubmed
CONCLUSION AND GENERAL SIGNIFICANCE : These results assist in the development of enzymatic synthesis approaches toward chimeric glycosaminoglycan
oligosaccharides and designing future strategies for directed evolution of
KfoC in order to create mutants toward user - defined goals . -
pubmed
We demonstrate the immobilization of
formate ,
formaldehyde , and
alcohol dehydrogenases on one - and - the - same electrode for direct
CO2 reduction . -
pubmed
This method of immobilization of
enzymes on electrodes offers the opportunity for electrochemical application of enzymatic electrodes to many reactions in which a substitution of the expensive sacrificial co - enzyme
NADH is desired . -
pubmed
Isoprene Side - chain of SMTP is Essential for
Soluble Epoxide Hydrolase Inhibition and Cellular Localization . -
pubmed
Here , we investigate the yet - to - be - characterized function of the
isoprene side- chain of
SMTPs in
sEH inhibition and cellular distribution . -
pubmed
The introduction of a terminal
hydroxy group exceptionally unaffected
epoxide hydrolase , but led to impaired cellular localization , resulting in diminution of cellular
epoxide hydrolase inhibition . -
pubmed
Thus , the
isoprene side - chain of
SMTP is an important pharmacophore for
epoxide hydrolase inhibition and cellular localization . -
pubmed
Women receiving
mifepristone and
misoprostol could choose office follow - up with an ultrasound evaluation one to two weeks after
mifepristone or telephone follow - up with a scheduled telephone interview at one week
post abortion and a second telephone call at four weeks to review the results of a home urine pregnancy test . -
pubmed
Soluble Receptors for
Tumor Necrosis Factor and
Neopterin as Parameters of Cell - Mediated Immune Activation . -
pubmed
In
cancer and
autoimmune diseases , measurement of
sTNF - Rs and
neopterin provides useful information for monitoring . -
pubmed
The quantification of
sTNF - Rs and
neopterin concentrations often provide even superior information than that obtained with classical disease markers , probably due to the direct involvement of the "
interferon - gamma / tumor necrosis factor - alpha system " in the pathogenetic mechanisms of the diseases . -
pubmed
Data imply that measurement of
sTNF - Rs , especially of the 75kD - type , is a useful adjunct for quantification of the Th-1 type immune response , sharing many characteristics with
neopterin . -
pubmed
MicroRNA-21 drives severe ,
steroid - insensitive experimental
asthma by amplifying
phosphoinositide 3 - kinase - mediated suppression of
histone deacetylase 2 . -
pubmed
Treatment with
Ant-21 , or the pan -
PI3 K inhibitor
LY294002 , reduced
PI3 K activity and restored
HDAC2 levels . -
pubmed
BACKGROUND :
Alkaptonuria (
AKU ) is an
ultra - rare inborn error of metabolism characterized by
homogentisic acid (
HGA ) accumulation due to a deficient activity of the
homogentisate 1.2 - dioxygenase (
HGD ) enzyme . -
pubmed
METHODS : Gel electrophoresis , Western Blot , Congo Red - based assays and electron microscopy were used to investigate the effects of
HGA on the aggregation and fibrillation propensity of amyloidogenic proteins and peptides [
Abeta ( 1 - 42 ) ,
transthyretin ,
atrial natriuretic peptide ,
alpha - synuclein and
SAA ] . -
pubmed
LC /
MS and in silico analyses were undertaken to identify possible binding sites for
HGA ( or its oxidative metabolite , a
benzoquinone acetate or
BQA ) in
SAA . -
pubmed
RESULTS : We found that
HGA might act as an amyloid aggregation enhancer in vitro for all the tested proteins and peptides in a time- and dose- dependent fashion , and identified a small crevice at the interface between two
HGD subunits as a candidate binding site for
HGA /
BQA . -
pubmed
Influence of
HDL particles on cell -
cholesterol efflux under various pathological conditions . -
pubmed
In controls , there were strong positive correlations between
ABCA1 - dependent
cholesterol efflux and small prebeta-1 concentrations (
R2 = 0.317 ) and
SR - BI - dependent
cholesterol efflux and large ( alpha-1 + alpha-2 )
HDL particle concentrations (
R2 = 0.774 ) . -
pubmed
HDL anti - oxidative capacity was negatively influenced by high plasma
beta - sitosterol levels , but not by the concentrations of
HDL particles ,
TG ,
SAA ,
fibrinogen , or
MPO . -
pubmed
BIIB021 : A novel inhibitor to
heat shock protein 90 - addicted oncology . -
pubmed
BIIB021 is an orally available , fully synthetic novel small - molecule
heat shock protein 90 inhibitor that has shown strong antitumor activities in a large number of preclinical models and is now under clinical investigation . -
pubmed
Body composition , physical fitness ( 1,200 running , sit - ups , back strength , sit and reach , side - steps ) and blood variables (
low - density lipoprotein cholesterol ,
glucose , reactive
oxygen species , and
malondialdehyde ) were examined before and after 16 weeks of training . -
pubmed
The rationale is based on the assumption that
DMSO supplementation should induce long - term negative effects on both pre- and
post - implantation embryo development . -
pubmed
Methods :
DMSO induced oxidative stress ,
ER stress , autophagy , mitophagy , signaling responsible genes and proteins were determined by RT - qPCR , Western blotting , immunofluorescence , and confocal microscopy . -
pubmed
Notably ,
DMSO - treated 2 - cell embryos showed increased expression of unfolded protein response genes such as
Hspa5 ,
Hsp90b1 ,
Ddit3 ,
Atf4 , and
Xbp1 . -
pubmed
Further ,
DMSO - induced endoplasmic reticulum stress increased mitochondrial
Ca2 + levels , induced mitochondrial depolarization / dysfunction , and induced apoptotic cell
death via the
JNK /
ATF2 - dependent pathway . -
pubmed
Consequently , treatment with
DMSO increased the expression of autophagy initiation- , phagophore elongation- , and autophagosome formation - related genes , as well as localization of
PINK1 /
Parkin , which are the main mediators of mitophagy , in mitochondria . -
pubmed
Finally , exogenous
lactate administration can significantly attenuate cell
death in neuronal cultures following exposure to lethal
OGD . -
pubmed
l - NAME administration led to increased blood pressure ( BP ) ( 30 % ) , decreased
nitric oxide synthase (
NOS ) activity ( 50 % ) , and increases in
NADPH oxidase (
NOX)-dependent
superoxide anion production ( 60 % ) and
p47phox protein level ( 65 % ) . -
pubmed
The co - administration of
quercetin prevented the increase in BP and the activation of
NOX but did not modify the decrease in
NOS activity caused by
l - NAME . -
pubmed
In addition ,
quercetin affected oxidative stress parameters as
glutathione oxidation , and the activities of oxidant detoxifying enzymes
superoxide dismutase ,
glutathione peroxidase , and
catalase . -
pubmed
Thus ,
quercetin administration counteracts
l - NAME effects on
NO bioavailability determinants in vivo , essentially through controlling
NOX - mediated
superoxide anion production . -
pubmed
This study uses an in vitro system , consisting of monoculture and direct co - cultures of monocytes and
MSC on screw - shaped machined and oxidized
titanium implants in combination with scanning electron microscopy , enzyme - linked immunosorbent assay , flow cytometry , cell sorting , and quantitative
polymerase chain reaction . -
pubmed
Functional roles of
tyrosine 185 during the
bacteriorhodopsin photocycle as revealed by in situ spectroscopic studies . -
pubmed
Tyrosine 185 ( Y185 ) , one of the aromatic residues within the
retinal (
Ret ) chromophore binding pocket in helix F of
bacteriorhodopsin (
bR ) , is highly conserved among the microbial
rhodopsin family proteins . -
pubmed
In the state - of - the - art red QLED , an ultrathin insulating layer inserted between the QD layer and the
oxide electron - transporting layer (
ETL ) is crucial for both optimizing charge balance and preserving the QDs ' emissive properties . -
pubmed
Suppressing exciton quenching at the
ETL - QD interface , which is identified as being obligatory for high - performance devices , is achieved by adopting
Zn0.9 Mg0.1 O nanocrystals , instead of
ZnO nanocrystals , as ETLs . -
pubmed
Optimizing charge balance is readily addressed by other device engineering approaches , such as controlling the
oxide ETL / cathode interface and adjusting the thickness of the
oxide ETL . -
pubmed
Results :
Hesperidin improved self - renewal ability and chondrogenesis of MSCs , inhibited secretion of pro - inflammatory
cytokines IFN - gamma ,
IL-2 ,
IL-4 and
IL-10 , and suppressed the expression of
p65 . -
pubmed
Overexpression of
p65 was able to reverse the
hesperidin inhibited secretions of pro - inflammatory
cytokines , and abolish the enhancing effect of
hesperidin on chondrogenesis of MSCs . -
pubmed
In this study , the OmniChange multi - site mutagenesis method was applied to reengineer the
P450 BM3 substrate specificity towards the hydroxylation of
CTAB by simultaneous mutagenesis of four previously reported positions ( R47 , Y51 , F87 , and L188 ) . -
pubmed
1740 clones from the
P450 BM3 OmniChange library were screened with the
NADPH depletion assay . -
pubmed
A total of 696 clones were rescreened with the
NADPH depletion and an
Ampliflu Red/
horseradish peroxidase based
H2 O2 detection assay . -
pubmed
Several improved
P450 BM3 variants were identified and finally four were kinetically characterized with respect to
CTAB hydroxylation , based on both performance and coupling efficiency . -
pubmed
Based on
NADPH consumption , the
P450 BM3 variant
P3A8 ( R47E / Y51M / F87V / L188E ) displayed an initial activity ( 64.9 + -4.8 s-1 , 13.5 - fold increased activity compared with wild - type
P450 BM3 ) , which nearly matches the specific activity for its natural
fatty acid substrate (
palmitic acid ( 32 - 122 s-1 ) ) . -
pubmed
HPLC -
MS /
MS detection confirmed that
P3A8 and P2E7 ( R47D / Y51L / F87V / L188A ) form 13 and 35 times more
2 - hydroxylated CTAB than
P450 BM3 . -
pubmed
In addition ,
di - hydroxylated CTAB products were detected for all four investigated
P450 BM3 variants ( up to a yield of 77 % ; P3A8 ) . -
pubmed
The present study assessed the effect of Contingency Management ( CM ) combined with Cognitive - Behavioral Treatment (
CBT ) on certain in - treatment behaviors ( treatment retention , in - treatment smoking abstinence , and weekly decrease of
cotinine levels ) and examined the effects of these in - treatment behaviors on smoking status at a 6 - month follow - up . -
pubmed
A
CT scan revealed an enhanced hypertrophied wall of the short segment of the small intestine , and
99 m Tc - labeled
human serum albumin scintigraphy revealed protein leakage into the thickened wall of the small intestine . -
pubmed
CIH also resulted in hippocampus neuron apoptosis with increased
caspase 3 level , dUIP nick end labeling ( TUNEL)-positive neurons number and
cognitive impairment verified by prolonged latency and shortened time in the target quadrant in Morris water maze test . -
pubmed
However , pretreatment of
NF - kappaB inhibitor
BAY 11 - 7082 inhibited the activation of
NF - kappaB under CIH condition and also significantly reduced the phosphorylation of
JNK as well as
c - Jun ,
ATF2 , and
JunD. -
pubmed
AlkB monooxygenases in bacteria are responsible for the hydroxylation of medium- and long - chain
n - alkanes . -
pubmed
Hexadecyl CoA and
octadecyl CoA could effectively release the
CrgA binding and start the transcription of
alkB2 gene , implying a positive regulation of metabolic intermediate . -
pubmed
18F - Fludeoxyglucose PET /
CT in
SCLC : Analysis of the CONVERT Randomized Controlled Trial . -
pubmed
INTRODUCTION : We used phase-3 CONVERT trial data to investigate the impact of
fludeoxyglucose F 18 (
18F - FDG ) positron emission tomography ( PET)/computed tomography (
CT ) in
SCLC . -
pubmed
However , this analysis can not support the use or omission of
18F - FDG PET /
CT owing to study limitations . -
pubmed
Novel
btk inhibitors such as
acalabrutinib ,
zanubrutinib , and
tirabrutinib are designed to improve on the safety and efficacy of first - generation
btk inhibitors such as
ibrutinib . -
pubmed
Data from single - arm clinical trials suggest that , compared with
ibrutinib , second - generation
btk inhibitors have comparable efficacy and might have a more favourable toxicity profile . -
pubmed
Heat shock protein 70 (
HSP70 ) is an
ATP - dependent molecular chaperone , and it has been shown that its levels increase after exposure to various types of stress , including
ischemia . -
pubmed
In addition ,
Tat -
HSP70 treatment significantly reduced lipid peroxidation and increased
Cu ,
Zn - superoxide dismutase activities in the spinal cord , but
glutathione peroxidase and
Mn - superoxide dismutase activities remained unchanged . -
pubmed
Salicylic acid (
SA ) mediated defence response was up - regulated in symptomless roots , compared with root gall tissue . -
pubmed
The
PbBSMT derived
Methyl - SA potentially leads to increased pathogen tolerance in uninfected roots . -
pubmed
When cultivated on
M1 , all isolates produced EPS exhibiting almost the same
monosaccharide profile with
mannose ,
glucose and
arabinose being the main monomers . -
pubmed
Various characterization techniques would demonstrate the successful preparation of resultant
CNC -
PhE - PEGMA with uniform nanoscale size , remarkable fluorescent properties and extremely low
cytotoxicity . -
pubmed
In this work , the interfacial friction angle of the polymer pellets in the partially plasticized samples of
polymethyl methacrylate (
PMMA ) ,
polypropylene (
PP ) , and
nylon66 (
PA66 ) were characterized by a super - high magnification lens zoom 3D microscope . -
pubmed
While allosensitization can occur when a patient comes in contact with foreign antigens , this problem appears more common in female recipients of haploidentical stem cell transplants , due to the development of
anti - HLA antibodies during pregnancy against offspring 's
HLA antigens . -
pubmed
The incidence of DSAs in haploidentical transplants is approximately 15 - 20%;1 however , can be as high as 42.9 % in parous females.2 Until now , only allosensitization of the recipient against donor
HLA antigens has been studied . -
pubmed
Nevertheless , multiparous female donors are not uncommon in haploidentical transplant setting , and may carry a similar risk of developing anti -
HLA antibodies against recipient 's
HLA antigens on the unshared haplotype . -
pubmed
To investigate if these genes are involved in defense against the
macrocyclic lactone insecticide
ivermectin in this insect , each gene was silenced using RNA interference or
MDR protein activity was inhibited using a chemical inhibitor ,
verapamil , before challenging the insects with a dose of
ivermectin . -
pubmed
BACKGROUND :
Tislelizumab is an investigational , humanized ,
IgG4 monoclonal antibody with high affinity and binding specificity for
programmed cell death-1 (
PD-1 ) that was engineered to minimize binding to
FcgammaR on macrophages in order to abrogate antibody - dependent phagocytosis , a mechanism of T - cell clearance and potential resistance to anti -
PD-1 therapy . -
pubmed
Relaxed hair was treated with different MW peptides derived from
keratin protein and an
amino acid ,
L - Leucine . -
pubmed
Iso - C15 : 0 ,
C15 : 0 anteiso , and summed feature 3 ( C16 : 1 omega6c and/or C16 : 1 omega7c ) were found to be the primary cellular
fatty acids in
strain C33T. -
pubmed
Male residents with low education levels suffer from larger effects of
PM1 and
SO2 on the incidence rate of
lung cancer . -
pubmed
Both gain- and loss - of - function studies , including CCK-8 ,
EdU assay , transwell experiment and
wound - healing assay were used to investigate the role of
ANXA1 in
PTC progression . -
pubmed
Besides , the combination of
cobicistat -
abacavir -
rilpivirine HIV drugs demonstrated the highest in silico efficacy of inhibiting
SARS - CoV-2 spike glycoprotein . -
pubmed
In order to establish standardized
MS - based screening assays , we studied 28 sequences with documented NMR structures in ( usually ~100 mM )
potassium , and report here their circular dichroism (
CD ) , melting temperature ( Tm ) , NMR spectra and electrospray mass spectra in 1 mM
KCl/100 mM
trimethylammonium acetate . -
pubmed
In group I the basal body temperature ( BBT ) ( P less than 0.001 ) and the plasma
progesterone concentrations ( P less than 0.05 ) were significantly elevated on the 1st day following the
luteinizing hormone (
LH ) peak . -
pubmed
In group
II , THE BBT was slightly elevated on the 1st day following the
LH peak ( P less than 0.05 ) , but the increase in the plasma
progesterone concentrations occurred only on the 2nd day following the
LH peak . -
pubmed
A method for the determination of
estradiol metabolic clearance rate ( MCR ) and production rate (
PR ) in blood , using radioactive isotope dilution techniques , has been described . -
pubmed
Hemodynamic profiles , arterial blood gases , plasma
glucose , and
serum amylase were obtained before laparotomy , at bile injection , and at hourly intervals . -
pubmed
C8H10N2O3 , Mr = 182.18 , monoclinic , P2(1 ) , a = 6.6405 ( 7 ) , b = 7.9493 ( 9 ) , c = 8.3662 ( 9 ) A , beta = 103.07 ( 1 ) degrees , V = 430.18 ( 8)
A3 , Z = 2 , Dx = 1.41 Mg m-3 , lambda (
Cu K alpha ) = 1.54184 A , mu = 0.879 mm-1 , F(000 ) = 192 , T = 298 K , R = 0.037 for 1247 reflections with Fo greater than or equal to 4 sigma ( Fo ) . -
pubmed
The configuration at
C7 is
S. -
pubmed
Under
LD 12:12 and reversed
LD 12:12 cycles , superfused pineal glands showed a rhythmic
melatonin secretion : Scotophase was associated with high titers and photophase with low titers . -
pubmed
However , the forward transmission of
Nd - YAG energy through the sapphires varied ( SMTR , 85 % ;
MTR , 83 % ; MTRL , 75 % ; OS , 54 % ; LT , 69 % ) . -
pubmed
Substance P ( 1 microM ) reduced basal secretion of
SLI in agreement with earlier studies . -
pubmed
Addition of
pyrilamine during
substance P perfusion increased
IRG secretion whereas addition of
cimetidine resulted in a delayed decrease on removal of both compounds . -
pubmed
Histamine ( 1 and 10 microM ) increased
SLI secretion and reduced
IRG secretion . -
pubmed
Pyrilamine increased and
cimetidine decreased
IRG secretion but neither drug influenced
SLI secretion . -
pubmed
Pyrilamine had no effect on
histamine - stimulated
SLI secretion but inhibition of
IRG secretion by
histamine was converted to stimulation . -
pubmed
Cimetidine potentiated
histamine stimulation of
SLI secretion and inhibition of
IRG secretion . -
pubmed
IN CONCLUSION : ( 1 )
substance P inhibition of
SLI secretion is unlikely to be mediated via release of
histamine . -
pubmed
( 3 )
Histamine weakly stimulates
SLI secretion but there may be both inhibitory and stimulatory pathways acting via
H2 - and H1 - receptors , respectively . -
pubmed
These data show that intracellular
methicillin selection facilitates isolation of mutations in genes required for intracellular growth and strengthens the premise that
listeriolysin O is essential for intracellular growth . -
pubmed
Peak A did not correspond to either
bradykinin or other known
kinins , such as
lysyl - bradykinin and
T - kinin , whereas peak B corresponded to
bradykinin . -
pubmed
After hydrophobic chromatography on
Octyl Sepharose CL 6B , separation from other contaminating
maltases was achieved by gel filtration on Ultrogel ACA 22 . -
pubmed
Transient induction of single
GST - P positive hepatocytes by
DEN . -
pubmed
Neither
acyclovir , FIAU , nor the
herpes thymidine kinase alone is harmful to cells . -
pubmed
The urinary
gamma - glutamyl transpeptidase - to -
creatinine ratio in
spot samples was simple and quick to measure and was correlated with the 24 - hour enzyme excretion . -
pubmed
Lubrol PX inhibited
enzyme activity , and
phospholipid reversed this inhibition . -
pubmed
Kinetic and spectroscopic data on the reaction of
selenite radicals with some
proteins and parallel inactivation studies generally reflect knowledge on the
amino acid residues mainly involved in the radical attack . -
pubmed
The investigations at different pH values on the reactivity of
selenite radicals with
amino acids and
proteins and on the transient spectra of the reaction products exhibit different behaviour for the various acid - base structures of the
selenite radicals , reflecting the influence of particular ionizable groups in the reacting molecules and the structure modifications at the level of proteins . -
pubmed
Characterization and mechanism of formation of reactive products formed during
peroxidase - catalyzed oxidation of
p - phenetidine . -
pubmed
OFF products of
horseradish peroxidase (
EC 1.11.1.7)-catalyzed oxidation of
p - phenetidine were isolated and reactive species were trapped with
reduced glutathione (
GSH ) and
butylated hydroxyanisole (
BHA ) . -
pubmed
Butorphanol , an exogenous opiate which preferentially generalizes to the
kappa - sigma opiate receptors , is a potent initiator of feeding . -
pubmed
In these studies , we examined the effect of peripherally administered putative satiety substances , cholecystokininoctapeptide ,
somatostatin ,
bombesin ,
gastrin - releasing peptide , thyrotropin - releasing hormone , calcitonin and glucagon on
butorphanol induced feeding . -
pubmed
This effect is mimicked by the incubation of normal fibroblasts with
dehydroepiandrosterone , a strong inhibitor of
glucose-6 - phosphate dehydrogenase . -
pubmed
Acetylcholinesterase is functional at tonic fibre endplates , but the absence of the
collagen - tailed form may account for the lower efficiency of the enzymic removal of
acetylcholine there . -
pubmed
Our approach was to combine
desmin staining with identification of
vitamin A ( autofluorescence ) , lipid droplets ( Sudan III ) ,
vimentin ,
laminin and
tenascin , using cryostat sections : Immunofluorescence , double - immunofluorescence or immunoperoxidase techniques were used . -
pubmed
The activation of
porcine pancreatic lipase by
cis - unsaturated fatty acids . -
pubmed
The overall angle of the
fatty acid , the position of its double bond and the presence of a
carboxyl group were critical factors in whether the
fatty acid effectively increased
lipase activity . -
pubmed
When the substrate is emulsified by
taurodeoxycholate , the pH optimum for
lipase ranges from 6.2 to 7.0 . -
pubmed
In the presence of
cis - unsaturated fatty acids , the overall activity of
lipase increases , the pH optimum shifts , and the pH - activity curve becomes biphasic , with one optimum around pH 7.7 , and the other around pH 8.8 . -
pubmed
Fluorescence studies indicate that
fatty acids bind near aromatic residues in
lipase , particularly
tryptophan . -
pubmed
10(-4 ) M. The far - UV CD data also show that
cis - unsaturated fatty acids change the conformation of
lipase . -
pubmed
Because the three - dimensional crystal structure of
lipase is known , a model is proposed to describe how
cis - unsaturated fatty acids increase
lipase activity . -
pubmed
Through the release of a variety of relaxing and contracting factors , including
nitric oxide and
endothelin-1 , the endothelium exerts a complex paracrine influence on vascular smooth muscle cells . -
pubmed
column packed with 4 - microns Nova Pack
C18 using a mobile phase of
water -
acetonitrile -
triethylamine ( 700:300:4 , v / v / v ) ( adjusted to pH 7.5 with
orthophosphoric acid ) . -
pubmed
Effect of intense
angiotensin II suppression on the diuretic response to
furosemide during chronic
ACE inhibition . -
pubmed
Similarly , urine volumes , in response to
furosemide , increased after
captopril ( 238 + /- 90 versus 283 + /- 111 mL ,
P & lt ; .05 , and 245 + /- 78 versus 311 + /- 92 mL , P & lt ; .01 , 1 and 2 hours after dosing ) . -
pubmed
Oxidative stress can result in
glutathione depletion , lipid peroxidation , membrane damage and DNA strand breaks as well as activation of
proteases ,
nucleases and
protein kinases . -
pubmed
Analyse hat purified the toxin protein , we find that its
amino acid composition was similar with 135 - kDa
P1 protein , and its sequence of N - terminal 18
amino acid was same with that of part of the
P1 protein . -
pubmed
From these data we can suggest that
mGlu receptors act inversely to the
NMDA receptors in the dorso - lateral periaqueductal gray area and that
L - AP-3 is a partial agonist rather than an antagonist of
mGlu receptors within the periaqueductal gray area . -
pubmed
S - Hexylglutathione or the xenobiotic substrate analogue ,
2,4 - dinitrophenol , protects against inactivation of the
enzyme by
4 - FSB , whereas
S - methylglutathione has little effect on the reaction . -
pubmed
These experiments indicate that reaction occurs within the active site of the
enzyme , probably in the binding site of the xenobiotic substrate , close to the
glutathione binding site . -
pubmed
Incorporation of
[ 3,5 - 3H ]- 4 - FSB into the
enzyme in the absence and presence of
S - hexylglutathione suggests that modification of one residue is responsible for the partial loss of
enzyme activity . -
pubmed
DTT regenerates
cysteine from the reaction product of
cysteine and
4 - FSB , but does not reactivate the
enzyme . -
pubmed
These results show that modification of
Tyr 8 by
4 - FSB causes the partial inactivation of the
enzyme . -
pubmed
The pH dependence of the
enzyme - catalyzed reaction of
glutathione with
CDNB for the modified
enzyme , as compared with the native
enzyme , reveals an increase of about 0.9 in the apparent pKa , which has been interpreted as representing the ionization of
enzyme - bound
glutathione ; however , this pKa of about 7.4 for modified
enzyme remains far below the pK of 9.1 for the -
SH of free
glutathione . -
pubmed
Previously , it was considered that
Tyr 8 was essential for
GST catalysis . -
pubmed
In contrast , we conclude that
Tyr 8 facilitates the ionization of the
thiol group of
glutathione bound to
glutathione S - transferase , but is not required for enzyme activity . -
pubmed
The substitution at nucleotide 1168 ( G-->C ) generates one
amino acid difference at residue 390 ( Asp-->
His ) of the
envelope protein ( E ) . -
pubmed
Ancillary analyses of these trials implicate certain risk factors for the continued progression of
CAD assessed by coronary angiography ; although standard lipids such as total
cholesterol and
LDL - cholesterol are important risk factors , recent evidence suggests that
triglycride - rich lipoproteins are also important contributors to progression of
CAD , both in treated and untreated trial subjects . -
pubmed
Staphylococci express a 42 kDa cell - wall - associated protein which functions as a receptor for the mammalian
iron - binding glycoprotein
transferrin . -
pubmed
However , small intestinal RNA content in the infected protein - free dietary group administered
nucleoside - nucleotide mixture was higher ( P & lt ; 0.05 ) and tended to be higher relative to the infected
nucleotide - free 20 %
casein group administered
nucleoside - nucleotide mixture compared with the
rest of the groups . -
pubmed
Formation of the
ADP - insensitive phosphoenzyme intermediate in the
sarcoplasmic reticulum Ca2 + - ATPase of which both
Cys344 and
Cys364 are modified by
N - ethylmaleimide . -
pubmed
Sarcoplasmic reticulum vesicles were pretreated with
N - ethylmaleimide under the conditions in which both
Cys344 and
Cys364 (
SHD ) of the
Ca2 + - ATPase are selectively modified . -
pubmed
Effects of the modification on the transition of the phosphoenzyme intermediate (
EP ) from
ADP - sensitive form to
ADP - insensitive form and on the formation of
ADP - insensitive
EP from Pi were examined without added K+ . -
pubmed
At pH 7.0 - 8.0 in totally aqueous media , the
EP transition and the
EP formation from
Pi were almost completely inhibited by the
SHD modification . -
pubmed
The formation of
ADP - insensitive
EP from
ATP and from Pi in the
SHD - modified
enzyme occurred to some extent at pH 6.0 - 6.5 and were greatly increased by addition of
dimethyl sulfoxide at pH 6 . -
pubmed
The inhibition by the
SHD modification of the
EP formation from Pi in the absence of
dimethyl sulfoxide was attributed to a decrease in the equilibrium constant for the
EP formation from the
enzyme -
Pi - Mg complex . -
pubmed
When 40 % ( v / v )
dimethyl sulfoxide was present , almost all the phosphorylation sites in the
SHD - modified
enzyme were phosphorylated with
ATP at pH 6.0 or with Pi at pH 6.0 - 7.0 , and all the
EP formed was
ADP - insensitive . -
pubmed
These results lead to the possibility that the previously reported exclusion of water from the catalytic site upon the
EP transition and upon the
EP formation from the
enzyme -
Pi - Mg complex is inhibited by the
SHD modification . -
pubmed
The modification of
SHD , which brackets the phosphorylation site (
Asp351 ) , may provide a useful tool for the analysis of conformational changes at the phosphorylation site occurring in the catalytic cycle . -
pubmed
In contrast to the relatively stable
PTH1R mRNA expression , the
cAMP response to
PTH varies markedly with no response at day 5 and a marked response ( 80 - fold versus control ) by day 10 . -
pubmed
Since
cAMP signaling was relatively unchanged during this interval ( day 10 - 30 ) and stimulation of
adenylate cyclase only partially mimicked the
PTH effect on increased mineralization , other signaling pathways are likely to be involved in order to determine the specific anabolic response to short - term
PTH treatment during the differentiation process . -
pubmed
Also ,
major basic protein ,
nitric oxide , and
eotaxin were found in the tissue , and
eosinophil cationic protein and
eosinophil protein X levels were elevated in sera . -
pubmed
Putative fusogenic activity of
NSF is restricted to a
lipid mixture whose coalescence is also triggered by other factors . -
pubmed
It has recently been reported that
N - ethylmaleimide - sensitive fusion ATPase (
NSF ) can fuse protein - free liposomes containing substantial amounts of
1,2 - dioleoylphosphatidylserine (
DOPS ) and
1 , 2 - dioleoyl - phosphatidyl - ethanolamine (
DOPE ) ( Otter - Nilsson et al . -
pubmed
However , this activity of
NSF is resistant to inactivation by
N - ethylmaleimide and does not depend on the presence of
alpha - SNAP ( soluble
NSF - attachment protein ) . -
pubmed
Moreover , under the same conditions , either
alpha - SNAP , other proteins apparently unrelated to vesicular transport (
glyceraldehyde-3 - phosphate dehydrogenase or
lactic dehydrogenase ) or even 3 mM
magnesium ions can also cause lipid mixing . -
pubmed
This transition can be mimicked in vitro , by
F9 embryonal carcinoma ( EC ) cells treated with
retinoic acid , to form ( epithelial ) primitive or visceral endoderm , and then with
parathyroid hormone - related peptide (
PTHrP ) to induce the transition to ( mesenchymal ) parietal endoderm . -
pubmed
Dye injection showed that this complex regulation of
connexin43 is correlated with functional gap junctional communication . -
pubmed
This mature phenotype is reached gradually during development through phases in which A - type
potassium channels and
T - type calcium channels are transiently expressed . -
pubmed
A clear association between head
pain and the release of
calcitonin gene - related peptide (
CGRP ) was demonstrated . -
pubmed
Structure - activity relationship of
cinnamic acylsulfonamide analogues on the
human EP3 prostanoid receptor . -
pubmed
In Experiment 1 , the sipper tube conditioned stimulus (
CS ) contained
saccharin /
ethanol solution and was repeatedly paired with food as an unconditioned stimulus ( US ) . -
pubmed
The
CS - US paired group consumed more of the 0.1 %
saccharin-6 %
ethanol solution than did the
CS - US random group , revealing that autoshaping conditioned responses (
CR ) induce
ethanol drinking not attributable to pseudo - conditioning . -
pubmed
Peptide
antigen priming of naive , but not memory ,
CD8 T cells requires a third signal that can be provided by
IL-12 . -
pubmed
Challenge with
peptide Ag in the absence of adjuvant results in tolerance of
CD8 T cells specific for the
Ag . -
pubmed
Crystal structure of a
clavaminate synthase -
Fe ( II )- 2 - oxoglutarate - substrate -
NO complex : evidence for metal centered rearrangements . -
pubmed
Clavaminate synthase (
CAS ) , a
2 - oxoglutarate ( 2OG ) dependent dioxygenase , catalyses three steps in the biosynthesis of
clavulanic acid . -
pubmed
Decay products of the S(3 ) state of the
oxygen - evolving complex of
photosystem II at cryogenic temperatures . -
pubmed
The water - oxidizing complex of
photosystem II cycles through five oxidation states , denoted S(i ) ( ) ( i = 0 - 4 ) , during water oxidation to molecular
oxygen , which appears at the ( transient ) S(4 ) state . -
pubmed
Excitation of the
S ( 3 ) state by near - IR light at cryogenic temperatures induces among other signals a derivative - shaped
EPR signal at g= 5 [ Ioannidis , N. , and Petrouleas , V. ( 2000 ) Biochemistry 39 , 5246 - 5254 ] . -
pubmed
A
sorbitol dehydrogenase inhibitor of exceptional in vivo potency with a long duration of action :
1 -( R )-[ 4 -[ 4 -( 4,6 - dimethyl [ 1,3,5 ] triazin-2 - yl )- 2R,6S - dimethylpiperazin-1 - yl ] pyrimidin-2 - yl ] ethanol . -
pubmed
These results suggest that
eugenol may act by blocking
Abeta - induced -
Ca ( 2 + ) intake and provide a strong case for further pursuit of the therapeutic and prophylactic potentials of
RAG and its active principles for the management of
Alzheimer ' s disease . -
pubmed
In this paper , it was studied if osmotic shrinkage of platelets affects the changes in intracellular
calcium concentration ( [
Ca(2+)](i ) ) induced by
thrombin or
adenosine 5 ' diphosphate (
ADP ) . -
pubmed
The osmolarity of the solution was elevated by the addition of 200 mM
mannitol or
sucrose and after the stimulation with 0.1 IU / ml of
thrombin or 100 microM
ADP , [
Ca ( 2 +)]( i ) increases were compared . -
pubmed
RESULTS : It was found that hypertonic solutions decrease the [
Ca(2+)](i ) peak induced by the agonist : The increase of [
Ca ( 2 +)]( i ) in the presence of 200 mM
mannitol produced by 100 microM
ADP was 62+/-6 % and response to 0.1 IU / ml
thrombin was 74+/-7 % of the increase in isotonic control solution . -
pubmed
CONCLUSIONS : In spite of the stimulatory effect of hyperosmolarity on capacitative
calcium entry observed in platelets in which the
calcium stores were depleted with
thapsigargin , the full response of intracellular
calcium to the agonists tested (
ADP and
thrombin ) was reduced by an increase in osmolarity . -
pubmed
In a prospective , randomized , placebo - controlled 12 - wk study , we investigated the effect of oral hormone replacement therapy on
asymmetric dimethylarginine (
ADMA ) , an endogenous inhibitor of
nitric oxide synthase (
NOS ) , and an independent risk factor for
coronary heart disease . -
pubmed
In conclusion , unopposed oral
estradiol and
estradiol combined with
dydrogesterone or
trimegestone reduced plasma levels of the
NOS inhibitor
ADMA . -
pubmed
Whether the reduction of the
NOS substrate
arginine in the
E ( 2 )+ T group counteracts the potentially beneficial effect of
ADMA reduction or reflects increased
NO production remains to be investigated . -
pubmed
[ Study of an X
antigen complex from rhinopharyngeal exudate by means of an
antigen -
antibody reaction supported by erythrocytes ( passive hemagglutination reaction of the Cantacuzino - Angelescu type ) . -
pubmed
Aldolase and
glyceraldehyde-3 - phosphate dehydrogenase activity in
primaquine - sensitive erythrocytes . -
pubmed
mutans
LraI operon ,
sloABCR , which encodes components of a putative metal uptake system composed of
SloA , an
ATP - binding protein ,
SloB , an integral membrane protein , and
SloC , a solute - binding lipoprotein , as well as a metal - dependent regulator ,
SloR. -
pubmed
By Western blotting , addition of
Mn to the growth medium repressed
SloC expression in a wild - type
strain but not in a
sloR mutant . -
pubmed
Fe at 10 micro M supported growth of the wild - type
strain but not of a
sloA or
sloC mutant . -
pubmed
Mn at 0.1 micro M supported growth of the wild - type
strain and
sloR mutant but not of
sloA or
sloC mutants . -
pubmed
The combined results suggest that the
SloABC proteins transport both metals , although the
SloR protein represses this system only in response to
Mn . -
pubmed
When tested for their sensitivity towards a series of antibiotics , null
yts mutants were found to be sensitive to
bacitracin . -
pubmed
Real - time
polymerase chain reaction ( PCR ) experiments demonstrated that the presence of
bacitracin in the growth medium strongly stimulates the expression of the
ytsCD genes encoding the
ABC transporter and that this stimulation strictly depends on the
YtsA response regulator . -
pubmed
The
ywoA gene encodes a protein known to confer some resistance to
bacitracin on the bacterium . -
pubmed
We propose that ( i ) the
YtsCD ABC transporter exports the
bacitracin ; ( ii )
YwoA , the protein that contains an
acidPPc (
PAP2 or
PgpB ) domain , is not part of an
ABC transporter but competes with
bacitracin for the dephosphorylation of the
C55 - isoprenyl pyrophosphate (
IPP ) ; ( iii ) the two resistance mechanisms are independent and complementary . -
pubmed
Long - term therapeutic regimes with
ACTH and
cortisone in chronic
nonendocrine dyscrasias and the control of side reactions by intermittent and alternate low dosage with these pharmaceuticals . -
pubmed
Correlation between
oxygen - deficient center formation and volume compaction in synthetic
SiO2 glass upon
ArF or
F2 excimer - laser irradiation . -
pubmed
Correlations between the refractive - index change and the concentration of an
oxygen - deficient center (
ODC ) induced by thermal treatments and laser irradiation are examined to clarify the origin of laser - induced volume compaction in synthetic
SiO2 glasses . -
pubmed
A linear correlation between them was clearly observed for thermally induced
ODC , whereas no correlation was found for
ArF or
F2 laser irradiation . -
pubmed
Transfection of
phosphorothioate CAGA oligonucleotide , a CAGA antisense analog , inhibited Gly - BSA - induced
PAI-1 mRNA expression . -
pubmed
Cotransfection of
phosphorothioate CAGA oligonucleotides with
PAI-1 reporter vector also blocked Gly - BSA - induced
PAI-1 promoter
luciferase activity . -
pubmed
We propose that
Wun2 in
pole cells competes with somatic
Wun and
Wun2 for a common lipid
phosphate substrate , which is required by
pole cells to produce their survival signal . -
pubmed
On a quantitative basis the population of
molecules used as
immunogen always reacted most effectively with the homologous
anti - idiotypic antiserum . -
pubmed
Each cyanobacterial protein contains just one
arginyl residue , similarly located between sites 1 and 2 , that is essential for the redox interaction with
Photosystem I. -
pubmed
Recruited macrophages specifically upregulated mRNA encoding the guidance
molecule EphB3 and expressed
EphB proteins capable of binding
Ephrin B molecules in vivo and in vitro . -
pubmed
In vitro ,
EphB3 supported adult RGC axon outgrowth , and axons turned toward a source of this guidance
molecule . -
pubmed
We show instead that STDP at layer 4 to layer 2/3 synapses in somatosensory (
S1 ) cortex involves separate
calcium sources and coincidence detection mechanisms for LTP and LTD . -
pubmed
LTD was independent of postsynaptic
NMDA receptors and instead required group I metabotropic
glutamate receptors and
calcium from voltage - sensitive channels and
IP3 receptor - gated stores . -
pubmed
Downstream of postsynaptic
calcium , LTD required retrograde endocannabinoid signaling , leading to presynaptic LTD expression , and also required activation of apparently presynaptic
NMDA receptors . -
pubmed
The
enzyme showed essentially no activity toward
hydroxypyruvate ,
pyruvate , or
NADH . -
pubmed
The K(m ) of the
enzyme for
glyoxylate was 0.11 millimolar . -
pubmed
S - nitrosoglutathione (
SNOG ) accumulates
hypoxia inducible factor - l alpha in main pulmonary artery endothelial cells but not in micro pulmonary vessel endothelial cells . -
pubmed
A density functional theory ( DFT ) study was carried out to calculate
( 17 ) O ,
( 14 ) N and
( 2 ) H electric field gradient (
EFG ) tensors in accurate neutron diffraction structures of
alpha - glycine at 288 and 427 K. B3LYP is the used method and 6 - 311+G ( * ) and 6 - 311++G ( * * ) are the basis sets in the calculations of
EFG tensors at the sites of
( 17 ) O ,
( 14 ) N and
( 2 ) H nuclei in the monomer and the octameric cluster of
alpha - glycine at two temperatures . -
pubmed
The thermal decomposition of
poly ( beta , l - malic acid ) ,
poly ( alpha - methyl beta , l - malate ) , and ionic complexes of the
polyacid with
alkyltrimethylammonium salts was studied by
TGA , GPC , and FTIR and NMR spectroscopy . -
pubmed
In this study , the effect of
( S )- 3,4 - dicarboxyphenylglycine (
DCPG ) , a selective
mGlu8 receptor agonist , has been investigated in inflammatory and
neuropathic pain models in order to elucidate the role of
mGlu8 receptor in modulating
pain perception . -
pubmed
Intra - lateral
PAG ( S )- 3,4 - DCPG reduced
pain behaviour when administered 10 min before
formalin or carrageenan ; both the effects were blocked by intra - lateral
PAG MSOP . -
pubmed
Taken together these results suggest that stimulation of
mGlu8 receptors relieve
formalin and carrageenan - induced
hyperalgesia in inflammatory
pain , whereas it would seem less effective in established inflammatory or
neuropathic pain . -
pubmed
This article reviews the evidence of efficacy ( or lack thereof ) for two types of agents touted as antiaging therapies : antioxidants (
vitamin E ,
vitamin C , and
carotenoids ) and hormones (
growth hormone ,
testosterone ,
dehydroepiandrosterone , and
vitamin D ) . -
pubmed
The
luciferase was biotinylated with conventional biotinylation reagents , and the biotinylated
lysine sites were determined by liquid chromatography - tandem mass spectrometry . -
pubmed
The biotinylated
luciferase was stable when stored at 4 degrees C. The stability of synthetic
S - Cypridina luciferin was significantly improved by adding antioxidants to
Tris - HCl buffer . -
pubmed
The biotinylated
luciferase and
S - Cypridina luciferin were used in a model study of the immunoassay for
interferon - alpha . -
pubmed
Structural elucidation of this impurity by spectral data (
1H NMR ,
13C NMR ,
MS and IR ) has been discussed . -
pubmed
In conclusion ,
ligustrazine inhibits OVA induced
airway inflammation by modulating key master switches
GATA-3 and
T - bet that result in reversing the Th2
cytokine patterns in
asthma . -
pubmed
A
13C - detected
N - H dipolar coupling technique is introduced for uniaxially mobile membrane
proteins for orientation determination using unoriented samples . -
pubmed
LXR activation causes upregulation of genes involved in reverse
cholesterol transport ( RCT ) , including
ABCA1 and
ABCG1 transporters , in macrophage and intestine . -
pubmed
Meyer ) through repeated
silica gel column chromatographies and its chemical structure was determined as
( 20S )- protopanaxadiol 3 - O -[ 6 ''- O - acetyl - beta - D - glucopyranosyl ( 1 - -& gt ; 2 )- beta - D - glucopyranoside on the basis of several spectral and physical evidences including HMBC and FAB -
MS . -
pubmed
The antiradical capacities of 13 carotenoids (
CAR ) and
vitamin E are explored , by assessing CAR
- H bond dissociation energy . -
pubmed
Norfloxacin ,
trimethoprim /
sulfamethoxazole ,
cytokines ,
nitric oxide , expression levels of
nuclear factor ( NF )- kappaB and
inhibitor of NF - kappaB (
IkB - alpha ) , neutrophil oxidative burst , and rate of apoptotic events were determined . -
pubmed
Serum and ascitic levels of
tumor necrosis factor - alpha ,
interferon - gamma ,
interleukin-12 , and
nitric oxide were significantly lower in peak than in trough samples . -
pubmed
A correlation was present between serum
norfloxacins concentrations and
tumor necrosis factor - alpha ( r = -0.68 ; P & lt ; .001 ) ,
interferon - gamma ( r = -0.66 ; P & lt ; .001 ) ,
interleukin-12 ( r = -0.66 ; P & lt ; .001 ) , and
nitric oxide ( r = -0.68 ; P & lt ; .001 ) . -
pubmed
Serum
norfloxacin 's highest concentrations ( 1 + /- 0.5 microg / mL ) were achieved at 1 - 2 hours and concurred in time with the lower levels of
cytokines and
nitric oxide . -
pubmed
Intracellular
norfloxacin 's highest levels ( 2 + /- 1 microg / mL/10(7 ) cells ) were observed at 2 hours and concurred with a lower
NF - kappaB expression , a reduced anion
superoxide generation , and apoptotic rate in response to
phorbol myristate acetate . -
pubmed
Trimethoprim /
sulfamethoxazole did not significantly modulate
cytokine expression . -
pubmed
AIM : To discuss current concepts in the use of
fluoride and to determine how much
fluoride is sufficient for
caries prevention but also how much is too much . -
pubmed
Local availability of
glycine near
N - methyl - D - aspartate receptors ( NMDARs ) is partly regulated by
neuronal glycine transporter 1 (
GlyT1 ) , which can therefore modulate NMDAR function because binding to the
glycine site of the NMDAR is necessary for channel activation . -
pubmed
PKCbetaII , but not
PKCbetaI , was localized to the mitochondria , and inhibition of
PKCbeta significantly reduced UV - induced reactive
oxygen species ( ROS ) in MCs with high
PKCbeta expression . -
pubmed
Geldanamycin selectively targets the nascent form of
ERBB3 for degradation . -
pubmed
Cellular studies indicate that , similar to
EGFR , the
geldanamycin (
GA ) sensitivity of
ERBB3 and
HSP90 binding resides in the nascent state and is dependent on the presence of the kinase domain of
ERBB3 . -
pubmed
Geldanamycin disrupts the interaction of
ERBB3 and
HSP90 and inhibits
ERBB3 maturation at an early stage of synthesis , prior to export from the ER . -
pubmed
Studies with a photo - convertible fusion protein of
ERBB3 suggest
geldanamycin sensitivity at a later stage in maturation , possibly through the putative role of
HSP90 in structural proofreading . -
pubmed
As
TXNIP is strongly induced by
glucose , this molecule could be of consequence to disordered angiogenesis manifest in
diabetes mellitus . -
pubmed
Magnetic resonance ( MR ) guided optical breast imaging is a promising modality to improve the specificity of breast imaging , because it provides high - resolution quantitative maps of total
hemoglobin ,
oxygen saturation , water content , and optical scattering . -
pubmed
We also identify
Pellino 1 in a two - hybrid screen using
IKKepsilon as bait , and show that
IKKepsilon /
TBK1 activate
Pellino 1 in vitro by phosphorylating
Ser76 ,
Thr288 and
Ser293 . -
pubmed
Proteins were covalently immobilized onto mesoporous
TiO ( 2 ) film via
3 - aminopropyltriethoxysilane (
APTES ) , and protein loading onto
TiO ( 2 ) films was about four times greater than on planar glass substrates , which consequently improved the protein activity . -
pubmed
Specific binding assay between streptavidin /
biotin and between
PSA / anti -
PSA demonstrated that the mesoporous
TiO ( 2 )-based protein microarrays yielded higher fluorescence signals and were more sensitive with lower detection limits than microarrays based on planar glass slides . -
pubmed
To overcome these limitations , high - dose intrathecal
morphine was combined with a continuous intravenous (
IV ) postoperative
naloxone infusion . -
pubmed
The aim of the present chart analysis was to investigate the safety and efficacy of high - dose
ITOs combined with
IV naloxone compared with
IV opioid analgesia alone . -
pubmed
In the first 24 h , the
ITO group used 6.8 + -10.2
morphine equivalents ( mg
IV ) versus 76.1 + - 44.4 in the
IVM group ( P<0.05 ) . -
pubmed
CONCLUSIONS : High - dose
ITOs combined with a postoperative
IV naloxone infusion provided excellent analgesia for major pelvic surgery . -
pubmed
The
IV naloxone infusion combined with high - dose ITOs appeared to control opioid side effects without affecting analgesia . -
pubmed
A HBSP has been designed as a novel bifunctional enzyme model with
SOD and
GPx activity by host / guest - directed self - assembly of
MnTPyP - M - Ad and
6 - Te - diCD . -
pubmed
In the bifunctional
enzyme model , the
Mn ( III ) porphyrins act as efficient
SOD active sites and the
tellurol moieties endow
GPx activity . -
pubmed
RESULTS :
PI , BT and BV of biofilms treated by various concentrations of
ZnCl ( 2 ) were reduced significantly when compared with the DDW group ( p<0.05 ) . -
pubmed
PI , BT and BV of the 2.5mM
ZnCl ( 2 ) group was significantly higher than groups of 5 , 10 , 20mM
ZnCl ( 2 ) ( p<0.05 ) . -
pubmed
Relationship between dose , drug levels , and
D2 receptor occupancy for the atypical antipsychotics
risperidone and
paliperidone . -
pubmed
Repeated 2 - week treatment with
risperidone or
paliperidone did not produce lasting changes in
D2R binding potential in any region examined . -
pubmed
We found dose - dependent
D2R occupancy for both
risperidone and
paliperidone in both basal ganglia and cortical regions of interest . -
pubmed
High resolution crystal structure of
rat long chain hydroxy acid oxidase in complex with the inhibitor
4 - carboxy-5 -[( 4 - chlorophenyl ) sulfanyl ]- 1 , 2 , 3 - thiadiazole . -
pubmed
Its isozyme
glycolate oxidase (
GOX ) , has a role in the formation of
oxalate , which can lead to pathological deposits of
calcium oxalate , in particular in the disease
primary hyperoxaluria . -
pubmed
In comparison with a lower resolution structure of this
enzyme , binding of the inhibitor induces a conformational change in part of the
TIM barrel loop 4 , as well as protonation of the active site
histidine . -
pubmed
Plasma
nicotine was measured by
GC . -
pubmed
LC -
MS /
MS was used to measure the plasma levels of
cotinine and
trans-3 '- hydroxycotinine , and urine levels of
nicotine and its metabolites , total
4 -( methylnitrosamino )- 1 -( 3 - pyridyl )- 1 - butanol (
NNAL ) and
PAH metabolites (
2 - naphthol ,
1 - , 2 - , and
3 - hydroxyfluorenes ,
1 - , 2 - , 3 - , and
4 - hydroxyphenanthrenes , and
1 - hydroxypyrene ) . -
pubmed
Furthermore , biochemical parameters of the redox status (
malondialdehyde ,
oxLDL and TEAC ) and
C - reactive protein (
CRP ) in plasma and the concentrations of toxic gases and endotoxins in the air were determined . -
pubmed
MDA ,
oxLDL and
CRC levels were in both study groups similar . -
pubmed
Synthesis and biological evaluation of
ortho - aryl N - hydroxycinnamides as potent
histone deacetylase ( HDAC ) 8 isoform - selective inhibitors . -
pubmed
Enzyme inhibitory analysis demonstrated that some of the resulting compounds ( 22 b , 22 d , 22 f , and 22 g ) exhibited anti -
HDAC8 activity superior to
PCI34051 , a known
HDAC8 - specific inhibitor , with IC(50 ) values in the range of 5 - 50 nM. -
pubmed
A crossover from negative to positive junction magnetoresistance ( JMR ) is observed in p -
La0.7Ca0.3MnO3 /
SiO2 / n -
Si heterostructure in current perpendicular to film plane (
CPP ) geometry . -
pubmed
The specific aim was to demonstrate the involvement of the caspase family and to identify specific
caspases which are activated by
6 - hydroxydopamine (
6OHD ) treatment leading to
death of dDCN . -
pubmed
Western blot ( WB ) and double immunofluorescent analyses of
caspases-2 , - 3 , and - 8 were carried out in untreated and
6OHD - treated dDCN . -
pubmed
zVADfmk was used to determine if it could inhibit activation of
caspases-2 , - 3 , and - 8 in dDCN following
6OHD - mediated stress . -
pubmed
RESULTS : Our immunofluorescent and WB data showed that
6OHD triggered
caspases-2 and - 8 activation which in turn activated
caspase-3 leading to
death of dDCN . -
pubmed
Additionally , WB analysis revealed that
caspases-2 , - 3 , and - 8 activation was reduced by
zVADfmk in
6OHD - treated cells . -
pubmed
DISCUSSION : The study showed that
6OHD - induced toxicity triggered
caspase mediated
death of dDCN . -
pubmed
This finding is in support of previous studies using different
PD model showing that
6OHD can induce
caspases-2 and - 3 activation through apoptotic pathway and that both
caspases can activate
caspase-3 in
PD . -
pubmed
Anatoxin - a is one of the common and major cyanobacterial neurotoxins acting as a powerful agonist at
nicotinic acetylcholine receptors (
nAChR ) . -
pubmed
DESIGN , SETTING , PARTICIPANTS , & amp ; MEASUREMENTS : The prevalence of apparent treatment - resistant
hypertension among Reasons for Geographic and Racial Differences in
Stroke study participants treated for
hypertension ( n=10,700 ) was determined by level of estimated
GFR and
albumin - to -
creatinine ratio , and correlates of apparent treatment - resistant
hypertension among those participants with
CKD were evaluated . -
pubmed
CKD was defined as an
albumin - to -
creatinine ratio & gt;= 30 mg / g or estimated GFR<60 ml / min per 1.73 m(2 ) . -
pubmed
RESULTS : The prevalence of apparent treatment - resistant
hypertension was 15.8 % , 24.9 % , and 33.4 % for those participants with estimated
GFR & gt;= 60 , 45 - 59 , and & lt;45 ml / min per 1.73 m(2 ) , respectively , and 12.1 % , 20.8 % , 27.7 % , and 48.3 % for
albumin - to -
creatinine ratio<10 , 10 - 29 , 30 - 299 , and & gt;= 300 mg / g , respectively . -
pubmed
The multivariable - adjusted prevalence ratios ( 95 % confidence intervals ) for apparent treatment - resistant
hypertension were 1.25 ( 1.11 to 1.41 ) and 1.20 ( 1.04 to 1.37 ) for estimated
GFR levels of 45 - 59 and & lt;45 ml / min per 1.73 m(2 ) , respectively , versus & gt;= 60 ml / min per 1.73 m(2 ) and 1.54 ( 1.39 to 1.71 ) , 1.76 ( 1.57 to 1.97 ) , and 2.44 ( 2.12 to 2.81 ) for
albumin - to -
creatinine ratio levels of 10 - 29 , 30 - 299 , and & gt;= 300 mg / g , respectively , versus
albumin - to -
creatinine ratio<10 mg / g. After multivariable adjustment , men , black race , larger waist circumference ,
diabetes , history of
myocardial infarction or
stroke , statin use , and lower estimated
GFR and higher
albumin - to -
creatinine ratio levels were associated with apparent treatment - resistant
hypertension among individuals with
CKD . -
pubmed
Logistic regression showed that compared with
FTF , pregnant women interviewed via ACASI were significantly more likely to self - report themselves as single ( 14 % versus 6 % ) , having & gt;5 sexual partners ( 35 % versus 29 % ) , having oral sex ( 42 % versus 35 % ) , using intravenous drugs ( 5 % versus 0 ) , smoking cigarettes ( 23 % versus 16 % ) , drinking
alcohol ( 13 % versus 8 % ) and using condoms during pregnancy ( 32 % versus 17 % ) . -
pubmed
Relationship of
apolipoproteins A-1 and B , and
lipoprotein ( a ) to cardiovascular outcomes : the
AIM - HIGH trial (
Atherothrombosis Intervention in
Metabolic Syndrome with Low HDL / High
Triglyceride and Impact on Global Health Outcomes ) . -
pubmed
OBJECTIVES : This study sought to examine the relationship between baseline and on - study
apolipoproteins ( apo ) A-1 and B and
lipoprotein ( a ) [
Lp ( a ) ] levels and the development of subsequent cardiovascular ( CV ) events in the
AIM - HIGH (
Atherothrombosis Intervention in
Metabolic Syndrome with Low HDL / High
Triglyceride and Impact on Global Health Outcomes ) trial . -
pubmed
BACKGROUND :
Niacin has been reported to lower
apoB and
Lp ( a ) and to raise
apoA-1 . -
pubmed
Baseline and on - study
Lp ( a ) were predictive of CV events in both
simvastatin plus placebo ( baseline HR : 1.24 [ p = 0.002 ] and on - study HR : 1.21 [ p = 0.017 ] ) and the
simvastatin plus
ERN group ( baseline HR : 1.25 [ p = 0.001 ] and on - study HR : 1.18 [ p = 0.028 ] ) . -
pubmed
The photoluminescence (
PL ) quenching and blue shift in the peak of the emission spectra occur due to the energy transfer from
Si NPs to
Au NPs . -
pubmed
Stress and withdrawal from
d - amphetamine alter
5 - HT2A receptor mRNA expression in the prefrontal cortex . -
pubmed
5 - HT2A receptor (
5 - HT2AR ) mRNA expression in the prefrontal cortex ( PFC ) is diminished following withdrawal from
d - amphetamine (
AMPH ) and may underlie the emotional and cognitive impairments observed in psychostimulant withdrawal , but whether stress affects
5 - HT2AR mRNA expression during psychostimulant withdrawal is unknown . -
pubmed
The goal of this study was to examine the impact of forced swim test ( FST ) exposure during
AMPH withdrawal on
5 - HT2AR mRNA expression in PFC . -
pubmed
These data indicate that stress and short - term
AMPH withdrawal affect prefrontal
5 - HT2AR mRNA expression to a similar degree , and stress experienced during long - term
AMPH withdrawal can diminish the recovery of
5 - HT2AR mRNA expression . -
pubmed
Together , these data suggest that exposure to stress during extended
AMPH withdrawal could prolong withdrawal - induced , 5 - HT2AR mRNA expression which could be related to
5 - HT2AR mediated deficits . -
pubmed
Here , we identified the
methyl -
CpG - binding domain protein 1
MBD1 as a physical and functional partner of the
H19 long noncoding RNA ( lncRNA ) . -
pubmed
The
H19 lncRNA -
MBD1 complex , through its interaction with histone
lysine methyltransferases , therefore acts by bringing repressive histone marks on the differentially methylated regions of these three direct targets of the
H19 gene . -
pubmed
[ Anti -
atherosclerosis role of
N - oleoylethanolamine in
CB2 ] . -
pubmed
As the results shown ,
OEA ( 10 and 50 micromol x L(-1 ) ) could induce the
CB2 protein and mRNA expression , but not 100 micromol x L(-1 ) . -
pubmed
The anti -
inflammation effect of
OEA is induced by
PPAR - alpha and
CB2 , suggesting that
CB2 signaling could be a target for anti -
atherosclerosis ,
OEA have wide effect in anti -
inflammation , it may have better therapeutic potential in anti -
inflammation in HUVECs , thus achieving anti -
atherosclerosis effect . -
pubmed
The diagnostic
phospholipid detected was
phosphatidylcholine and MK-9 (
H4 ) was found to be the predominant
menaquinone . -
pubmed
Gene expression measurements revealed that
paracetamol decreased the expression of antioxidant genes and increased the expression of
interleukin-1beta (
IL-1beta ) ,
IL-8 ,
tumor necrosis factor - alpha (
TNF - alpha ) and acute phase proteins . -
pubmed
Curcumin administration ameliorated
paracetamol - induced alterations in genes expression of antioxidant and inflammatory
cytokines . -
pubmed
Gymnemic acid has a
CAS number but its structure is not specified . -
pubmed
We describe a one -
pot strategy for the high yielding , operationally simple synthesis of fluorescent probes for
Zn2 + that bear biological targeting groups and exemplify the utility of our method through the preparation of a small library of sensors . -
pubmed
In both single and repetitive DE exposure studies ,
MaR1 significantly decreased bronchoalveolar lavage neutrophil infiltration ,
interleukin 6 ,
tumor necrosis factor alpha , and
chemokine C - X - C motif ligand 1 levels without altering repetitive DE - induced
bronchioalveolar inflammation or lymphoid aggregate formation . -
pubmed
RESULTS : No significant differences for the modelled increase in
CD4 + T - cell count ( placebo 15.3
CD4 + T cells / muL ( 95 % confidence interval ( CI ) [ 1.0 , 29.5 ] versus
maraviroc arm 22.9
CD4 + T cells / muL ( 95 % CI [ 7.4 , 38.5 ] p = 0.51 ) or alterations in the expression of markers for T - cell activation , proliferation and microbial translocation were found between the arms . -
pubmed
However ,
maraviroc intensification did increase the percentage of
CCR5 expressing
CD4 + and
CD8 + T - cells , and the plasma levels of the
CCR5 ligand
MIP-1beta . -
pubmed
In contrast , the percentage of ex - vivo apoptotic
CD8 + and
CD4 + T - cells decreased in the
maraviroc arm . -
pubmed
CONCLUSIONS :
Maraviroc intensification of cART did not increase
CD4 + T - cell restoration or decrease immune activation as compared to placebo . -
pubmed
Amyloid deposition in
AD dementia showed a preference for the
DMN , but high effect sizes were also observed for other neocortical ICNs , most notably the frontoparietal - control network . -
pubmed
The mechanisms involved included reduced oxidative stress , enhanced antioxidant capacity and restored
vascular endothelial growth factor expression , and
NO bioavailability . -
pubmed
Sequential extractions were applied to determine the forms of P in different particle - size fractions and to assess the potential bioavailability of particulate
phosphorus (
PP ) . -
pubmed
The
BAPP fluxes associated with the " truly suspended " fraction was approximately 200 times larger than the dissolved inorganic
phosphorus (
DIP ) flux . -
pubmed
Thus , the transfer of fine particles to the open sea is most probably accompanied by
BAPP release to the
DIP and can support greater primary and secondary production . -
pubmed
Zinc sensing by
metal - responsive transcription factor 1 (
MTF1 ) controls
metallothionein and
ZnT1 expression to buffer the sensitivity of the transcriptome response to
zinc . -
pubmed
Surprisingly , a greater number of genes were regulated by
zinc following
MFT1 knockdown , and most genes that responded to
zinc under both control and
MTF1 - depleted conditions had an augmented response in the latter condition . -
pubmed
Exceptions were the zinc effluxer
ZnT1 and a suite of metallothionein genes , suggesting that responses of other genes to
zinc are usually buffered by increases in these proteins . -
pubmed
We propose that
MTF1 heads a hierarchy of
zinc sensors , and through controlling the expression of a raft of metallothioneins and other key proteins involved in controlling intracellular
zinc levels ( e.g. -
pubmed
ZnT1 ) alters
zinc buffering capacity and total cellular
zinc content . -
pubmed
We tested and validated this model by overexpressing
metallothionein and observing the predicted curtailment in response of the
zinc - repressed
SLC30A5 (
ZnT5 ) promoter . -
pubmed
venezuelensis were obtained in
phosphate saline (
SS and SM ) and in
Tris -
HCl buffer (
TS and
TM ) , and were analysed by Western blotting . -
pubmed
Xanthine oxidase inhibitory activity of
nicotino /
isonicotinohydrazides : A systematic approach from in vitro , in silico to in vivo studies . -
pubmed
Among the different factors involved in regulation of serum
uric acid ,
xanthine oxidase (
XO ) is the best pharmacological target to control the levels of serum
uric acid as it catalyzes the final steps in
uric acid production . -
pubmed
Benzylidene nicotino /
isonicotinohydrazides ( 1 - 54 ) were synthesized by treating
nicotinic / isonicotinic hydrazides with substituted
aromatic aldehyde , and characterized by EI -
MS and
1H NMR . -
pubmed
Further in silico molecular docking was carried out to study the interactions of these inhibitors with catalytically important
amino acid residues in
XO . -
pubmed
HMS and drug - loaded HMS were characterized by differential scanning calorimetry ( DSC ) , X - ray powder diffraction ( XRPD ) , Fourier transform infrared spectroscopy ( FTIR ) , scanning electron microscopy ( SEM ) , transmission electron microscopy (
TEM ) and contact angle study . -
pubmed
The
NBD - Pen method is highly sensitive as well as selective and is suitable for targeting minimally modified
LDL compared with other existing methods . -
pubmed
Here , we focus on the invasive margin as defined by the region with
5 - aminolevulinic acid (
5ALA ) (
GliolanTM ) fluorescence at surgery beyond the
T1 enhancing region on magnetic resonance imaging ( MRI ) . -
pubmed
We conducted
hematoxylin and
eosin ( H&E ) , array real time
polymerase chain reaction ( PCR ) , and immunohistochemistry staining on various intra -
tumour regions of
glioblastoma to determine molecular heterogeneity between regions . -
pubmed
Mangiferin prevents the growth of
gastric carcinoma by blocking the
PI3K -
Akt signalling pathway . -
pubmed
Taken together , our findings indicate that
mangiferin effectively inhibits cell growth and induces apoptosis of
gastric cancer cells through inhibiting the
PI3K /
Akt pathways with relative safety , and may be used as a novel chemotherapeutic agent against
gastric cancer . -
pubmed
RESULT(S ): An implantation rate of 69.9 % , clinical pregnancy rate per transfer of 70.6 % , and live birth rate per transfer of 64.5 % were observed in 1,621 nondonor frozen cycles with the use of
SNP - based
PGT - A. -
pubmed
No statistically significant differences in pregnancy outcomes were found for single - embryo transfers (
SET ) versus double - embryo transfers with
SNP - based
PGT - A. -
pubmed
CONCLUSION(S ): Our findings that
SNP - based
PGT - A can mitigate the negative effects of maternal age on
IVF outcomes in cycles with transfer , and that pregnancy outcomes from
SET cycles are not significantly different from those of double - embryo transfer cycles , support the use of
SET when transfers are combined with
SNP - based
PGT - A. -
pubmed
BACKGROUND : Toxicological studies highlight the potential
neurotoxicity of
perfluoroalkyl substances (
PFAS ) during fetal development . -
pubmed
RESULTS : Each ln - increase in
perfluorononanoate (
PFNA ) at 8 years was associated with a 3.4 - point increase ( 95 % CI 0.4 ,
6.3 ) in metacognition score , indicating poorer function . -
pubmed
16 4903 ; Gu et al 2017
ACS Nano 11 4943 ) , here we study the
strain performance for zigzag
BlueP nanoribbons ( zBluePNRs ) with different edge chemical modifications . -
pubmed
The limit of detection ( LOD ) and limit of quantification ( LOQ ) of the sensor were 1.76 pM and 5.87 pM for
TNT and 1.74 pM and 5.80 pM for
tetryl , respectively . -
pubmed
The sensor could be selectively applied to various mixtures of
TNT with common energetic materials such as
RDX ,
HMX , and
PETN . -
pubmed
Here we report , for the first time , large volume analytical serial sectioning tomography of an organic composite coating using a
xenon Plasma Focused Ion Beam ( PFIB ) combined with secondary electron imaging , energy dispersive X - ray ( EDX ) spectrum imaging (
SI ) and electron backscattered diffraction ( EBSD ) . -
pubmed
During the seed - filling stage , the
AMF - host PC with a reduced P application rate increased the uptake of most nutrients compared to the non - AMF - host PC , and improved the remobilization efficiency of all nutrients except
Mn ,
Fe , and
Se , compared to the optimal P application rate . -
pubmed
Formaldehyde (
FA ) is involved in many biological processes and is closely connected with many diseases including
Alzheimer ' s disease and
cancer . -
pubmed
In this manuscript , the first lysosome - targeted CDs for ratiometric fluorescence detection of
FA were efficiently prepared from
dexamethasone and
1,2,4,5 - tetraaminobenzene through the microwave - assisted hydrothermal method . -
pubmed
With ISEL as our primary exposure variable , we performed multivariable regression models to evaluate the association between social support and prevalent
CKD [ estimated
glomerular filtration rate ( eGFR ) & lt ; 60 ml / min/1.73 m2 or
urine albumin -
creatinine ratio (
ACR ) & gt;=30 mg / g ] ,
eGFR decline , and
rapid renal function decline ( RRFD ) ( & gt ; 30 % decrease in
eGFR over approximately 10 years ) . -
pubmed
While induction of testicular T / D remarkably increased the level of
TNF - alpha in the ipsilateral ( torted ) and contralateral ( non - torted ) testes , intraperitoneal ( i.p ) administration of
dapsone ( 12.5 mg / kg ) significantly lowered the
TNF - alpha level ( p & lt ; 0.001 ) . -
pubmed
Additionally , after induction of T / D ,
SOD activity was notably decreased , whereas administration of
dapsone ( 12.5 mg / kg , i.p . ) -
pubmed
Significant
drug - AR - AE pair signals involving
gabapentin included :
drug abuser ,
multiple drug overdose , and substance - induced
psychotic disorder . -
pubmed
Significant drug
AR - AE signals involving
gabapentin and
pregabalin , but not
duloxetine , were :
ataxia , dependence ,
drug abuse , increased
drug tolerance , and
overdose . -
pubmed
Compared to
duloxetine ,
gabapentin had significantly greater odds of a co - report for an
AS - AE with
drug withdrawal syndrome ( OR : 6.55 ) ,
auditory hallucinations ( OR : 4.57 ) ,
delusions ( OR : 2.36 ) ,
euphoric mood ( OR : 5.45 ) ,
ataxia ( OR : 2.85 ) ,
drug abuser ( OR : 3.01 ) ,
aggression ( OR : 1.98 ) ,
psychotic disorder ( OR : 1.96 ) , and feeling abnormal ( OR : 1.31 ) . -
pubmed
Prevention of Bone Resorption by HA /
beta - TCP +
Collagen Composite after Tooth Extraction : A Case Series . -
pubmed
The purpose of this study was to investigate the clinical efficacy of the application of a
hydroxyapatite /
beta - tricalcium plus
collagen ( HA / beta - TCP +
collagen ) dental bone graft in dental sockets immediately after tooth extraction , so as to prevent
socket resorption . -
pubmed
HA /
beta - TCP +
collagen was inserted into all of the dental sockets immediately after extraction , and was covered with a flap . -
pubmed
In conclusion , the HA /
beta - TCP +
collagen graft demonstrated adequate safety and efficacy in dental socket preservation following tooth extraction . -
pubmed
Then , the rewired
TCA cycle was optimized by designing various strengths of
pyruvate carboxylase and
isocitrate dehydrogenase expression cassettes , resulting in a large increase in
alpha - KG production ( 24.66 g / L ) . -
pubmed
Correlation Between Single
Nucleotide Polymorphisms of an miRNA Binding Site in the 3'UTR of
PTEN and Risk of
Cervical Cancer Among the Han Chinese . -
pubmed
Results : After adjusting for age , body - mass index ,
alcohol consumption , smoking , and familial history of
cancer , the
PTEN rs34140758 A allele carriers were 1.47 times more likely to suffer from
cervical cancer than the C allele carriers ( odds ratio [ OR ] = 1.47 , 95 % confidence interval [ CI ] : 1.17 - 1.72 , p = 0.001 ) . -
pubmed
It has been established that the action of
MutT1 on
8 - oxo - dGTP ,
8 - oxo - GTP and
diadenosine polyphosphates is modulated by intermolecular interactions . -
pubmed
In order to further explore this and to elucidate the structural basis of its differential action on
8 - oxo - NTPs and unsubstituted
NTPs , the crystal structures of complexes of
MsMutT1 with
8 - oxo - dGTP ,
GMPPNP and
GMPPCP have been determined . -
pubmed
Analysis of the structural data led to the elucidation of a relationship between the arrangements of molecules observed in the crystals , molecular plasticity and the action of the
enzyme on
nucleotides . -
pubmed
Testosterone ,
dihydrotestosterone (
DHT ) ,
dehydroepiandrosterone (
DHEA ) and
androstenedione were measured by IA and LC -
MS /
MS before and 7 days after
rhCG injection . -
pubmed
Using the
PB method , all hormones showed a linear correlation , but proportional and systematic concordance errors were detected for
androstenedione , systematic errors for
testosterone and no errors for
DHEA and
DHT . -
pubmed
By the BA method , there was a trend of IA to overestimate
testosterone and
androstenedione and underestimate
DHEA and
DHT when compared to LC -
MS /
MS . -
pubmed
CONTEXT :
11beta - Hydroxysteroid dehydrogenase 1 (
11betaHSD1 ) reduces inert
cortisone into active
cortisol but also catalyzes
reverse dehydrogenase activity . -
pubmed
MAIN OUTCOME MEASURE(S ):
11beta - Reductase and
11beta - dehydrogenase activities were measured during infusion of
9,11,12,12 - [ 2H ] 4 - cortisol and
1,2 - [ 2H ] 2 - cortisone , respectively , on two occasions , once during saline infusion and once during a hyperinsulinemic - euglycemic
clamp . -
pubmed
CONCLUSIONS : Regeneration of
cortisol is up - regulated by
insulin in adipose tissue but not skeletal muscle . -
pubmed
In
obesity , the equilibrium between
11beta - reductase and
11beta - dehydrogenase activities likely promotes
cortisol accumulation in adipose , which may lead to adverse metabolic consequences . -
pubmed
We show here that the sandwiched - fusion strategy , which provides robust protection against proteolysis , affords an economical method to obtain large quantities of pure five MDPs and the transcription factor
Guy1 , in sharp contrast to otherwise unsuccessful recovery using the traditional
amino - fusion method . -
pubmed
The partial
amino acid sequence of the light chain of one of these antibodies was identical with the dominant antibody light chain sequence , while the light chain of the other antibody , also partially established , showed significant variations in the framework - associated regions with identical CDRI and
II . -
pubmed
This report severe to document the histologic features of fatal exposure to
sulfur dioxide and stresses the need to follow parameters of
pulmonary function in the nonfatal cases . -
pubmed
All demonstrable
enzymes and toxins of encapsulated staphylococci (
KS ) were removed by extraction with
guanidinium chloride . -
pubmed
Angiotensin converting enzyme (
ACE ) is a
halide activated peptidase produced mainly by endothelial cells and by activated monocytes . -
pubmed
These results suggest that
L - lactate is translocated through
CAD - inhibitable new pathways induced in the host cell membrane by parasite activity , probably by diffusion of the acid form and through a modified native
monocarboxylate : H + symporter . -
pubmed
Continuous monitoring of extracellular pH changes occurring upon suspension of infected cells in isoosmotic
Na - lactate solutions indicates that part of the
lactate egress is mediated by anionic exchange through the constitutive , but modified ,
anion exchanger . -
pubmed
Anesthesia was induced with
SF , 6 micrograms / kg , while breathing 100 %
oxygen . -
pubmed
The structure and stereochemistry of
16 - hydroxytriptolide was established as
L2 on the basis of spectral data ( IR ,
MS , UV , 1H - NMR , 13C - NMR , 2d - NMR , NOE ) and X - ray crystallographic analysis . -
pubmed
Net mass transfer of
cholesteryl esters from
low density lipoproteins to
high density lipoproteins in plasma from normolipidemic subjects . -
pubmed
All plasma samples showed an increase in high density lipoprotein (
HDL )
cholesteryl esters during 1 - hour incubations . -
pubmed
Transfer of
triglycerides occurred from
VLDL +
LDL to
HDL in plasma from all subjects . -
pubmed
The
cholesteryl ester transfer ( measured after 1 hour ) is not related to the activity of
cholesteryl ester transfer protein . -
pubmed
Ultracentrifugal separation of
plasma lipoproteins revealed that both
HDL and
VLDL are acceptors of
cholesteryl esters and that
VLDL donates
triglycerides to both
LDL and
HDL . -
pubmed
Removal of
VLDL from plasma by ultracentrifugation did not affect the
cholesteryl ester transfer from
LDL to
HDL . -
pubmed
We conclude that
LDL may act as a donor of
cholesteryl esters during incubation of normolipidemic plasma . -
pubmed
Studies by flow cytometry have shown that these
antigens are found on the surface of epidermal and endothelial cells , and are modulated by
gamma interferon . -
pubmed
The first step is characterized by ( i ) destabilization of the secondary acceptor
semiquinone anion , Q - B , bound to the
D1 protein . -
pubmed
However , the slow phase ( microsecond ) of P+680 dark reduction increased , and the amplitude of signal
II was reduced by 20 - 30 % , indicating that in a fraction of RCII , electron donation from Z to
P + 680 was impaired without losing primary photochemistry . -
pubmed
In conclusion , reducing
catecholamine content or modifying
beta - noradrenergic receptor activity was not able to alter the
convulsive activity which occurs after acute
PINX . -
pubmed
[ Observation of oral
glucose -
insulin releasing test in
liver cirrhosis ] . -
pubmed
Blood
glucose and immunoreactive
insulin were determined in both groups at 60 min intervals for 180 min . -
pubmed
To attain complete
rest it is advisable to administer as premedication a psychopharmaceutical preparation and the neuroleptic preparation
Haloperidol in adequate doses . -
pubmed
Thermal stability of
myosin subfragment-1 decreases upon tryptic digestion in the presence of
nucleotides . -
pubmed
Myosin subfragment-1 ( S-1 ) , digested with
trypsin in the presence of
ATP , rapidly loses its
ATPase activity upon mild heat treatment even if
ATP or
ADP is present . -
pubmed
Direct measurement of
tubulin and bulk message distributions on polysomes of growing , starved and deciliated Tetrahymena using RNA gel blots of
sucrose gradients containing
acrylamide . -
pubmed
The major translation - level process regulating
tubulin synthesis in Tetrahymena appears to be a change in
message loading mediated by a non - specific message recruitment or unmasking factor . -
pubmed
Both
HP6069 or
HP6070 (
anti - IgG1 Fc ) worked well alone or together as capture antibodies in the
IgG1 IEMA : WR = 20 - 1250 ng / ml , S = 15 ng / ml . -
pubmed
HP6002 (
anti - gG2 Fc ) alone or in combination with
HP6014 (
anti - IgG2 Fd ) produced an
IgG2 IEMA with a
WR of 5 - 200 ng / ml and S of 5 ng / ml . -
pubmed
HP6047 ( anti -
IgG3 hinge ) alone generated a sensitive
IgG3 IEMA with a narrow working range : WR = 2 - 50 ng / ml , S = 1.6 ng / ml . -
pubmed
Both
HP6025 and
HP6023 (
anti - IgG4 Fc ) worked equally well alone and together to produce a useful
IgG4 IEMA : WR = 8 - 250 ng / ml , S = 7.8 ng / ml . -
pubmed
HP6017 (
anti - IgG PAN Fc ) was combined in an equal molar ratio with
HP6046 (
anti - IgG PAN Fd ) to produce a total
IgG PAN IEMA with a
WR of 5 - 530 ng / ml and a sensitivity of 5 ng / ml . -
pubmed
Propionyl - CoA carboxylase [
PCC ,
propanoyl - CoA : carbon - dioxide ligase (
ADP - forming ) ,
EC 6.4.1.3 ] is a
biotin - dependent enzyme involved in the degradation of branched - chain
amino acids ,
fatty acids with odd - numbered chain lengths , and other metabolites . -
pubmed
One class contained the anticipated
Ala - Met - Lys - Met sequence , corresponding to the
biotin binding site found in several
biotin - dependent
carboxylases , thus confirming the alpha - chain assignment of these clones . -
pubmed
All three parasite lines required
sialic acid for optimal invasion , but Thai-2 parasites cultured in Tn erythrocytes invaded
neuraminidase - treated erythrocytes with 45 % efficiency whereas
Camp parasites invaded
neuraminidase - treated erythrocytes with less than 10 % efficiency . -
pubmed
The experiments again call attention to the phenomenon that the
steroid hormones , even though specific for their target cells , are able to induce "
alien " imprinting when administered in the neonatal period . -
pubmed
Cell - bound isozymes of
penicillinase are distinguished from extracellular enzyme by their capacity to bind
deoxycholate and to elute with an apparent molecular weight of 45,000 on gel filtration in its presence . -
pubmed
In the case of plasma membrane
penicillinase , addition of 25 per cent
potassium phosphate at pH 9.0 leads to a 65 per cent conversion in 20 minutes at 30 degrees . -
pubmed
One epitope was located between
amino acid residues 145 - 168 of
VP1 and the other between
amino acids 169 - 179 . -
pubmed
Incubations of horizontal sections through the hippocampus with
[ 3H ] spiperone ( 1 nM ) resulted in dense labeling restricted to the pyramid cell layer in
CA1 , the parasubiculum and layers I and II of the entorhinal area ( EA ) , while the other hippocampal subfields contained moderate to low binding . -
pubmed
Complete traslocation of
IF from the cytoplasm to the nuclear compartment was evident in all normal endometrial cells exposed to
E2 ,
DES or
OH - TX for two hours . -
pubmed
While cells from the
endometrial cancer 'S ' , like the normal cells , translocated
IF to the nucleus , cells of another
cancer ( ' KLE ' ) failed to translocate when exposed to E2 or
OH - TX . -
pubmed
The results revealed the suitability of
anti - ER antibodies for investigating the intracellular dynamics of
ER in target cells responding to
estrogens or antiestrogens . -
pubmed
Reductive methylation of the
lysyl residues in the
fd gene 5 DNA - binding protein :
CD and
13C NMR results on the modified protein . -
pubmed
The
epsilon - amino groups of the six
lysyl residues of the
fd gene 5 DNA - binding protein have been modified by reductive methylation to form
N epsilon , N epsilon - dimethyl lysyl derivatives containing
13C - labeled
methyl groups . -
pubmed
13C NMR spectra at 50.1 MHz of
[ 13C ] methylated gene 5 protein show five distinct resonances between 43.30 and 42.76 ppm originating from the six N epsilon ,
N epsilon - dimethyl lysyl residues . -
pubmed
The optimal pH and
phosphate concentration for the separation of
hemoglobin from the membranes were found to be 7.5 and 5 mM , respectively . -
pubmed
Sodium dodecyl sulphate (
SDS ) electrophoretic analysis of the membrane
proteins prepared by centrifugation and by this new method shows similar
protein compositions . -
pubmed
The isolated cDNA was identical in both nucleotide and
amino acid sequences with the recently cloned liver
dihydrodiol dehydrogenase ( Stolz , A. , Hammond , L. , Lou , H. , Takikawa , H. , Ronk , M. , and Shively , J.E. -
pubmed
The
glycosylphosphatidylinositol ( GPI)-anchored membrane protein
urokinase plasminogen activator - receptor (
uPA - R ;
CD87 ) is one of the key molecules involved in migration of leukocytes and
tumor cells . -
pubmed
Activation of monocytes even with enzymatically inactivated
uPA resulted in induction of
tyrosine phosphorylation , suggesting modulation of
uPA - R - associated PTKs upon ligand binding . -
pubmed
[ The
DEC-205 receptor expressed by dendritic cells and thymic epithelial cells is implicated in
antigen processing ] . -
pubmed
Acetylcholinesterase inhibition by
zifrosilone : pharmacokinetics and pharmacodynamics . -
pubmed
Serial blood samples were obtained for
zifrosilone plasma concentrations and red blood cell
acetylcholinesterase and
butyrylcholinesterase activities . -
pubmed
Butyrylcholinesterase activity was relatively unaffected by
zifrosilone ( & lt ; 20 % inhibition at 300 mg ) . -
pubmed
For doses & gt ; or = 200 mg , an Emax pharmacodynamic model was used to describe the relationship between
zifrosilone plasma concentration and red blood cell
acetylcholinesterase inhibition ( Emax = 83.8 % ; EC50 = 0.65 ng / ml ) . -
pubmed
CONCLUSIONS :
Zifrosilone showed dose - dependent pharmacokinetics after oral administration and was effective in causing selective inhibition of
red blood cell acetylcholinesterase . -
pubmed
Metabolism of
cholecystokinin-33 in vivo : effect of
L-364,718 , a
CCK receptor antagonist . -
pubmed
Pretreatment of animals with
erythro-9 -) 2 - hydroxy-3 - nonyl ) adenine (
EHNA ) , an inhibitor of
adenosine deaminase , also potentiated the increased concentrations of
adenosine in venous blood by 6 - fold and raised arterial
adenosine concentrations by 3 - fold . -
pubmed
These data clearly suggest that
hemorrhage induced extensive
adenosine release and the released endogenous
adenosine was eliminated quickly from plasma by the uptake mechanism and
adenosine deaminase . -
pubmed
Immunogold labeling of thylakoid vesicles , with antibodies to synthetic peptides , shows that residues 413 - 421 of
PSI - A are exposed on the stromal surface of the membrane , and that the accessibility of this region is enhanced by
NaSCN treatment , which removes extrinsic polypeptides . -
pubmed
Assuming that the transmembrane regions are correctly predicted by the 11 - helix model , the N - terminal domain , as well as the conserved region proposed to bind the
iron - sulfur center
FX , would be expected to be on the stromal surface . -
pubmed
This level of interaction is in agreement with the functional behaviour of natural
haemoglobin mutants with mutations at the
2,3 - diphosphoglycerate binding site . -
pubmed
It was observed that during growth of this organism ,
sucrose in the medium is hydrolysed to
glucose and
fructose , suggesting the formation of an extracellular
invertase . -
pubmed
The
invertase was found to be the only predominant extracellular protein in the culture broth and could be purified in a single step by precipitation at 90 %
ammonium sulphate saturation . -
pubmed
It not only showed
invertase activity , but also a
fructosyltransferase activity as it could convert
sucrose to
beta-1,2 - difructose , as well as to
glucose and
fructose . -
pubmed
The power spectrum analysis of R - R interval variability ( RRV ) has been estimated by means of an autoregressive method in six men in supine ( S ) and sitting ( C ) postures at
rest and during steady - state cycle exercise at about 14 % , 28 % , 45 % , 67 % of the maximal
oxygen consumption ( % VO2max ) . -
pubmed
Substitution of
alanine543 with a
threonine residue at the
carboxy terminal end of the beta - chain is associated with thermolabile
hexosaminidase B in a Jewish family of Oriental ancestry . -
pubmed
The beta - chain thermolability was found to be associated with the presence of a G --> ; A transition at nucleotide 1627 of the
HEX B gene causing the substitution of
Ala543 with a
threonine . -
pubmed
Cb1 , the protein product of c - cbl proto - oncogene , has been reported to be phosphorylated on
tyrosine residues upon
T cell receptor (
TCR ) engagement . -
pubmed
However , in lymphocytes activated through the
TCR ,
Cbl loses its ability to bind to
Grb2 precipitated either with anti -
Grb2 antibody or with an immobilized
tyrosine phosphopeptide , Y1068 -
P , derived from the epidermal growth factor receptor . -
pubmed
Additionally , high levels of hydrophobic
amino acids were present , accounting for the hydrophobic nature of
Bac1829 . -
pubmed
The same effect was seen in the
tps1 (
trehalose-6 - phosphate synthase )
hsp104 double mutant , suggesting that the extreme heat
shock sensitivity was due mainly to a lack of
trehalose rather than to the presence of
trehalose-6 - phosphate . -
pubmed
However , accumulation of
trehalose-6 - phosphate did cause some phenotypes in the
tps2 mutant , such as temperature sensitivity for growth . -
pubmed
Finally , we isolated a high copy number suppressor of the temperature sensitivity of
tps2 , which we call
PMU1 , which reduced the levels of
trehalose-6 - phosphate in
tps2 mutants . -
pubmed
CR3 may function to activate cells by presenting
LPS to a downstream signal transducer . -
pubmed
We conclude that in a selected group of patients without
CC resistance and normal ovarian response following
CC stimulation [ maximum of three follicles with a diameter of & gt;16 mm at the time of administration of
human chorionic gonadotrophin (
HCG ) ] , IUI combined with
CC - HCG can be offered as a very safe and non - expensive first - line treatment , at least with an IMC of & gt;1 x 10(6 ) spermatozoa . -
pubmed
Only beta - sheet , positive ion salt bridges between
phosphates , and hydrophobic association can create tangles of just
AD P - tau . -
pubmed
We have correlated transmission electron microscope (
TEM ) images of
tau aggregation with different percentages of beta - sheet in aqueous suspensions of
tau while using buffers that block dispositive or tripositive ionic bridges between intermolecular
phosphates . -
pubmed
Each preparation except one demonstrated an inverse temperature transition ,
Ti , in the
CD at 197 nm . -
pubmed
Occupation of five to nine
phosphate sites on
AD P - tau appears sufficient to reduce or neutralize tau 's basic character . -
pubmed
The
Ti for
AD P - tau was 24.5 degrees C or 28 degrees C , whereas for
B tau with three
phosphates it was 32 degrees C , for unphosphorylated
tau R-39 it was 38 degrees C , and for dephosphorylated
HF AD P - tau it was 37.5 degrees C. The hydrophobic protein
elastin and its analogs coalesce and precipitate at their
Ti of 24 - 29 degrees C , well below body temperature . -
pubmed
Combination therapy with
thymosin alpha1 and
interferon for the treatment of
chronic hepatitis C infection : a randomized , placebo - controlled double - blind trial . -
pubmed
We evaluated the addition of
thymosin alpha1 (
TA1 ) , an immunomodulatory peptide , to the standard treatment regimen for
hepatitis C to determine if combination therapy shows biological activity using outcome measures including normalization of
alanine aminotransferase levels , histological activity , and viral load during treatment . -
pubmed
The
polyrotaxanes regulated
thrombin - induced
calcium increase in platelets although constituent molecules of the polyrotaxanes showed fewer effects on the intracellular metabolism . -
pubmed
The mutation , a single A-->G base substitution at nucleotide 5519 of the
SCN5A cDNA , is expected to cause a nonconservative change from an
aspartate to a
glycine at position 1790 ( D1790 G ) of the
SCN5A gene product . -
pubmed
Induction of the cell cycle regulatory gene
p21 (
Waf1 ,
Cip1 ) following
methylmercury exposure in vitro and in vivo . -
pubmed
Both embryonic LB and CNS cells responded to
MeHg exposure with a concentration - dependent increase in
p21 mRNA . -
pubmed
The
ethanol extraction procedure concentrated
CP , gross energy , and TMEn . -
pubmed
7alpha,15alpha - Dihydroxyderivative was the only product isolated from the
5 - androsten-3beta - ol post - transformation mixture . -
pubmed
The ( -)-
nicotine ( 3x10(-5 ) M)-induced
noradrenaline release was abolished by
tetrodotoxin and
hexamethonium and partially blocked by
dihydro - beta - erythroidine ( up to 10(-5 ) M ) ( a relatively selective antagonist of
alpha4beta2 nicotinic receptors ) and abolished by
mecamylamine ( 10(-5 ) M ) ( a relatively selective antagonist of
alpha3beta4 nicotinic receptors ) , but not influenced by alpha - bungarotoxin ( 3x10(-7 ) M ) or alpha - conotoxin ImI ( 10(-6 ) M ) ( antagonists of
alpha7 nicotinic receptors ) . -
pubmed
( + /-)- Epibatidine ( 3x10(-7 ) M ) ( a very potent , but non - selective agonist ) and
( -)- cytisine ( 3x10(-4 ) M ) ( an agonist of
beta4 nicotinic receptors ) effectively evoked the release of
noradrenaline , while
( E )- N - methyl-4 -( 3 - pyridinyl )- 3 - butene-1 - amine (
RJR-2403 ) ( up to 10(-4 ) M ) ( an agonist of
alpha4beta2 nicotinic receptors ) had no effect . -
pubmed
These subjects with low hematocrit had also higher values of the following parameters : aerobic working capacity ( p & lt ; 0.01 ) ; isometric adductor strength ( p = 0.02 ) ; crossover point of
carbohydrate oxidation ( 70 %
carbohydrates/30 % lipids ) ( p & lt ; 0.05 ) ;
insulin like growth factor binding protein 1 ( p & lt ; 0.0001 ) . -
pubmed
parasiticus resulted in the loss of
fluP transcript , a 3- to 4 - fold reduction in hyphal growth rate , the appearance of a fluffy , cotton - like hyphal morphology , reduction or elimination of asexual spores and spore - bearing structures , and a twofold reduction in
aflatoxin accumulation . -
pubmed
The role of antiganglioside
IgM is to eliminate the immunosuppressive
gangliosides shed from tissues during ageing , degeneration of neural and extraneural tissues , and
tumor growth and
necrosis . -
pubmed
Cyr61 is overexpressed ,
estrogen - inducible , and associated with more advanced disease . -
pubmed
Differential screening of the library isolated the growth factor - inducible immediate - early gene
Cyr61 , a secreted ,
cysteine - rich , heparin binding protein that promotes endothelial cell adhesion , migration , and neovascularization . -
pubmed
Induction of
Cyr61 mRNA was blocked by
tamoxifen and
ICI182,780 , inhibitors of the
estrogen receptor . -
pubmed
In summary , these results suggest that
Cyr61 may play a role in the progression of
breast cancer and may be involved in
estrogen - mediated
tumor development . -
pubmed
Kinetic analysis of
[ ( 11 ) C ] MP4A using a high - radioactivity brain region that represents an integrated input function for measurement of cerebral
acetylcholinesterase activity without arterial blood sampling . -
pubmed
[ ( 11 ) C ] MP4A is specifically hydrolyzed by
AChE in the brain to a hydrophilic metabolite , which is irreversibly trapped locally in the brain . -
pubmed
These regions , because of their high
AChE activity , act as a biologic integrator of plasma input function during PET scanning , when regional metabolic rates of
[ ( 11 ) C ] MP4A through
AChE ( k(3 ) ; an
AChE index ) are calculated by using Blomqvist 's linear least squares analysis . -
pubmed
The sensitivity of
RLS analysis for detecting cortical regions with abnormally low k 3 in the 26 patients with
AD ( 138 of 312 regions , 44 % ) was somewhat less than NLS analysis ( 52 % ) , but was greater than shape analysis ( 33 % ) , another method of
[ ( 11 ) C ] MP4A kinetic analysis without blood sampling . -
pubmed
The authors conclude that
RLS analysis is practical and useful for routine analysis of clinical
[ ( 11 ) C ] MP4A studies . -
pubmed
Pycnogenol protects neurons from
amyloid - beta peptide - induced apoptosis . -
pubmed
Reactive
oxygen species ( ROS ) has been proposed to be involved in the apoptotic mechanism of
Abeta - mediated
neurotoxicity . -
pubmed
We further found that
PYC not only suppressed the generation of ROS but also attenuated
caspase-3 activation , DNA fragmentation ,
PARP cleavage , and eventually protected against
Abeta - induced apoptosis . -
pubmed
Vitamin E also suppressed cell
death and
caspase-3 activation induced by
Abeta(25 - 35 ) . -
pubmed
A novel subclass of
thalidomide analogue with anti - solid
tumor activity in which
caspase - dependent apoptosis is associated with altered expression of
bcl-2 family proteins . -
pubmed
The antitumor activity was independent of known
PDE4 inhibitory activity and did not involve
cAMP elevation . -
pubmed
Thyrotropin (
TSH , 10 mU / ml ) and free
calcium ( 0.17~1.95 mM ) , which are associated with the biological behavior of thyrocytes and C cells , respectively , did not affect the events described above . -
pubmed
The data indicate that thyrocytes , but not C cells , actively undergo growth and three types of mother follicle - derived folliculogenesis at the tissue periphery in a
TSH- or free
calcium - independent manner . -
pubmed
Detection of
nitric oxide (
NO ) by the cellular biosensing system was used as a readout for assessing the immunomodulating effects of various chemicals , because some are known to induce
NO synthase (
iNOS ) activity thereby increasing
NO production . -
pubmed
The result of preoperative stress -
rest Tc-99m - MIBI SPECT myocardial perfusion study is an exact predictor of the state of perfusion measured early ( & lt ; 5 months ) after CABG ; the postoperative regional perfusion improvement is most dependent upon reversibility and also upon severity of stress defect . -
pubmed
Preoperative viability assessment on the basis of
Tc-99m - MIBI study performed solely in
rest is unjustified : at the similar perfusion defect at
rest , the presence of even minimal inducible
ischaemia is associated with increased probability of perfusion improvement after CABG . -
pubmed
Induction by
hypoxia combined with low
glucose or low
bicarbonate and high posttranslational stability upon reoxygenation contribute to
carbonic anhydrase IX expression in
cancer cells . -
pubmed
We found that both
hypoxia - induced transcription and
CA IX protein level are further increased by reduced
glucose or
bicarbonate concentrations . -
pubmed
However , by birth , the
SN DA neurons were labeled exclusively by
DiA placed in the dorsolateral striatum , and the VTA DA neurons were labeled only by
DiI injected into the ventromedial striatum . -
pubmed
Compared with
CCK2R ,
CCK2i4svR contains an additional 69
amino acids within its third intracellular loop ( 3il ) domain . -
pubmed
The peptides
P1 ,
P2 , and
P3 revealed a significant stimulatory effect on [
35S]GTPgammaS binding in control temporal cortex . -
pubmed
The most potent stimulator ,
P3 , at 10 microM concentration enhanced
[ 35S ] GTPgammaS binding by 500 % . -
pubmed
Glutathione ,
desferrioxamine (
DFO ) , and
17beta - estradiol markedly decreased the strong stimulatory effect by
P3 on
[ 35S ] GTPgammaS binding to control temporal cortex , with the protective effect by
DFO being most potent . -
pubmed
An
IV bolus of
lidocaine 2 mg / kg was then administrated and venous plasma
lidocaine concentrations were measured to determine pharmacokinetic values . -
pubmed
,
OxyR ) and a
low - molecular - weight heat shock protein (
HSP33 ) , whose activity increases upon oxidation of critical
sulfhydryl residues . -
pubmed
Depletion of cellular
reduced glutathione may act as a signal for
HO-1 transcriptional activation . -
pubmed
HO-1 protein expression can occur in the lung in response to oxidative stress associated with
infection , altered
oxygen tension , and
inflammatory diseases . -
pubmed
Thiol - reactive
clenbuterol analogues conjugated to
bovine serum albumin . -
pubmed
Several novel
thiol - reactive
clenbuterol analogues were coupled in high yield with
bovine serum albumin (
BSA ) . -
pubmed
After labelling of unreacted
cysteines with
maleimide spin label ( MiSL ) , the yield of the coupling reaction was determined by electron paramagnetic resonance (
EPR ) spectroscopy and spectral analysis . -
pubmed
Molecular dynamics was used to model the structure of
clenbuterol analogues and spin label conjugated to
BSA and recognition of conjugates by anti -
clenbuterol antibodies was demonstrated . -
pubmed
The recognition of
BSA - A ,
BSA - C and BSA - S conjugates with monoclonal and polyclonal anti -
clenbuterol (
mCLB - Ab and rCLB - Ab ) antibodies was an indication , that
chlorine substituents on the aromatic ring of
clenbuterol derivatives are not necessary for the binding of
antibodies to the conjugates . -
pubmed
We show that an identical toeprint was found for the
chloramphenicol acetyltransferase transcript using this nonradioactive method , which is in agreement with the previously published
32P - labeled method . -
pubmed
Optimization of the safety and efficacy of
interferon beta 1b and
azathioprine combination therapy in
multiple sclerosis . -
pubmed
Linopirdine was developed as a cognitive enhancing molecule and demonstrated to specifically block the
potassium current generated by the brain specific
KCNQ2 -
KCNQ3 proteins (
M - channel ) . -
pubmed
The testing of
CYP450 ligands on the
M - channel activity , using a
Rb(+ ) efflux assay on cells expressing the
KCNQ2 -
KCNQ3 proteins , demonstrated that
[ ( 3 ) H ] linopirdine binding results can not be correlated to
M - channel inhibition . -
pubmed
Thermal dissipation of excess absorbed light energy , measured as non - photochemical quenching (
NPQ ) of
chlorophyll fluorescence , is induced rapidly in response to excess light conditions , and it is known that xanthophylls such as
zeaxanthin and
lutein , the transthylakoid pH gradient , and the
PsbS protein are involved in this mechanism . -
pubmed
reinhardtii genes encoding
PsbS and
lycopene varepsilon - cyclase , and we show that the
lor1 mutation , which affects
lutein synthesis , is located within the
lycopene varepsilon - cyclase gene . -
pubmed
Pulmonary function tests were as follows ( mean+/-sd ): total lung capacity 88%+/-17 , forced vital capacity ( FVC ) 88%+/-18 , forced expiratory volume in one second ( FEV1 ) 80%+/-21 ( % predicted ) , FEV1 / FVC 69%+/-13 ,
carbon monoxide diffusion capacity of the lung 37%+/-16 ( % predicted ) ,
carbon monoxide transfer coefficient 46%+/-19 . -
pubmed
The aim of this study was to enhance the efficiency of transfection and expression in BCECs of DNA / polymer nanoparticles with the modification of membrane - penetrating peptide ,
Antennapedia peptide ( Antp )
polyethylenimine (
PEI ) and
polyamidoamine (
PAMAM ) were chosen to prepare
Antp - modified DNA - loaded nanoparticles with a complex coacervation technique . -
pubmed
After a 20 - min transfection , the efficiency , in terms of transfection and expression , of DNA /
PEI NP or DNA /
PAMAM NP was enhanced significantly with the modification of
Antp . -
pubmed
After a 3 - h transfection of DNA /
Antp /
PEI NP , there was no difference in cellular uptake but an enhancement in gene expression , compared to DNA /
PEI NP alone . -
pubmed
However , both the transfection and expression efficiency of DNA /
PAMAM NP were enhanced using
Antp . -
pubmed
Cyanidase is a new enzyme preparation capable of degrading
cyanide in industrial wastewaters to
ammonia and
formate in an apparently one - step reaction , down to very low concentrations . -
pubmed
This
enzyme has both a high selectivity and affinity toward
cyanide . -
pubmed
The kinetics of the enzymatic degradation of
cyanide were studied in a batch reactor using the powdered immobilized
enzyme preparation and modeled using a simple Michaelis - Menten equation . -
pubmed
Heme - binding to the nuclear receptor
retinoid X receptor alpha (
RXRalpha ) leads to the inhibition of the transcriptional activity . -
pubmed
Heme acts as a ligand for
transcription factors and regulates the expression of several genes . -
pubmed
We here show that
heme binds to
RXRalpha and impairs its DNA - binding activity . -
pubmed
The DNA - binding activity using the DR-1 consensus sequence of
RXRalpha in electrophoretic mobility shift assays was inhibited by
heme . -
pubmed
However , the inductions of adipogenic factor mRNAs including
PPAR - gamma ,
fatty acid binding protein-4 and
glucose transporter-4 were markedly repressed by
heme during adipocyte differentiation . -
pubmed
Fast pressure jumps can perturb
calcium and
magnesium binding to
troponin C F29W. -
pubmed
We have used rapid pressure jump and stopped - flow fluorometry to investigate
calcium and
magnesium binding to F29W
chicken skeletal troponin C. -
pubmed
We sequenced the encoded complex I units :
ND2 ,
ND3 ,
ND4 ,
ND5 and
ND6 genes and the
mitochondrial ATPase 6 ,
tRNA ( Val ) , tRNA(
Leu(UUR ) ) , tRNA(Trp ) and tRNA(
Lys ) genes in 10 unrelated patients with
Leigh syndrome . -
pubmed
Although the mechanism of idiosyncratic
hepatotoxicity remains unknown , it is proposed that metabolic activation of
nevirapine and subsequent covalently binding of reactive metabolites to cellular
proteins play a causative role . -
pubmed
These findings are consistent with a bioactivation sequence involving initial
P450 - catalyzed dehydrogenation of the aromatic nucleus with a
4 - methyl group in
nevirapine to an electrophilic
quinone methide intermediate , which is subsequently attacked by
glutathione yielding the
sulfydryl conjugate . -
pubmed
Formation of the
nevirapine GSH conjugate was primarily catalyzed by heterologously expressed recombinant
CYP3A4 and , to a lesser extent ,
CYP2D6 ,
CYP2C19 , and
CYP2A6 . -
pubmed
In addition , the
quinone methide reactive metabolite was a mechanism - based inactivator of
CYP3A4 , with inactivation parameters K(I ) = 31 microM and k(inact ) = 0.029 min(-1 ) , respectively . -
pubmed
Phosphorylation of
myristoylated alanine - rich C kinase substrate is involved in the
cAMP - dependent
amylase release in parotid acinar cells . -
pubmed
The involvement of
MARCKS phosphorylation in secretory function has been reported in
Ca ( 2 +)-mediated exocytosis . -
pubmed
The beta - adrenergic agonist
isoproterenol (
IPR ) induced
MARCKS phosphorylation in a time - dependent manner . -
pubmed
Translocation of a part of phosphorylated
MARCKS from the membrane to the cytosol and enhancement of
MARCKS phosphorylation at the apical membrane site induced by
IPR were observed by immunohistochemistry . -
pubmed
H89 , a
cAMP - dependent protein kinase (
PKA ) inhibitor , inhibited the
IPR - induced
MARCKS phosphorylation . -
pubmed
The
PKCdelta inhibitor
rottlerin inhibited the
IPR - induced
MARCKS phosphorylation and
amylase release . -
pubmed
IPR activated
PKCdelta , and the effects of
IPR were inhibited by the
PKA inhibitors . -
pubmed
A
MARCKS - related peptide partially inhibited the
IPR - induced
amylase release . -
pubmed
These findings suggest that
MARCKS phosphorylation via the activation of
PKCdelta , which is downstream of
PKA activation , is involved in the
cAMP - dependent
amylase release in parotid acinar cells . -
pubmed
R (+) XK469 inhibits hydroxylation of
S - warfarin by
CYP2C9 . -
pubmed
INTRODUCTION :
XK469 is a novel
topoisomerase II inhibitor structurally akin to several
propionic acid derivatives , such as
ibuprofen and
diclofenac , which are metabolised by
CYP2C9 . -
pubmed
The aim of the study is to investigate whether
R (+) XK469 interacts with
S - warfarin by inhibition of
CYP2C9 . -
pubmed
This suggests that coadministration of
R (+) XK469 and
warfarin results in a clinically significant pharmacokinetic interaction due to
CYP2C9 inhibition by
R (+) XK469 . -
pubmed
Although there was a significant correlation between
BMD and SUV in the lumbar spine at baseline ( P & lt ; 0.05 ) , there was no correlation between the 2 variables at 12 mo of treatment with
alendronate . -
pubmed
Aerobic
ammonia- and
nitrite - oxidising bacteria were verified in water column samples through Most Probable Number (
MPN ) analysis , but they were not detected in sediment samples by FISH . -
pubmed
The
SRL interacts with translation factors that hydrolyze
GTP , and it is important for their binding to the ribosome , but its precise role is not yet understood . -
pubmed
alpha - Sarcin - treated ribosomes showed no defects in mRNA and
tRNA binding , peptide - bond formation and
sparsomycin - dependent translocation . -
pubmed
Importantly , cleavage of
SRL inhibited
EF - G binding , and consequently
GTP hydrolysis and mRNA -
tRNA translocation . -
pubmed
We report on a screening of 23 surgically removed ,
cortisol - secreting ATs for the expression of receptors for
luteinizing hormone (
LH ) ,
gastric - inhibitory polypeptide (
GIP ) , and
vasopressin (
V ( 1a ) , V ( 1b ) , and
V ( 2 ) ) . -
pubmed
In addition , polymorphisms have been described in modulatory systems involving the NMDAR , including the enzymes
serine racemase and
d - amino acid oxidase / G72 that regulate brain
d - serine synthesis . -
pubmed
BACKGROUND / AIMS : We have previously demonstrated that a transcription factor ,
upstream stimulatory factor 2 (
USF2 ) , regulates
glucose - induced
thrombospondin 1 expression and
transforming growth factor - beta activity in mesangial cells , and plays an important role in
diabetic glomerulopathy . -
pubmed
In this study , we determined whether
USF2 expression in renal proximal tubular cells is regulated by
glucose and contributes to
diabetic tubulointerstitial fibrosis . -
pubmed
In addition , high
glucose stimulated
angiotensinogen and
renin expression , increased
renin activity , and resulted in increased
angiotensin II formation . -
pubmed
AluYb8 insertion in the
MUTYH gene is related to increased
8 - OHdG in genomic DNA and could be a risk factor for
type 2 diabetes in a Chinese population . -
pubmed
Therefore , the
AluYb8MUTYH polymorphism could be a novel genetic risk factor for
T2DM , and accumulated
8 - OHdG could contribute to this disease . -
pubmed
Inverse probability weighting to control for censoring in a
post hoc analysis of quality - adjusted survival data from a clinical trial of
temsirolimus for
renal cell carcinoma . -
pubmed
Differences in mean QAS between
temsirolimus and
IFN - alfa were evaluated with t - statistics at a two - sided alpha = 0.05 . -
pubmed
Overall weighted mean ( standard deviation ) QAS during progression - free survival was 111.9 ( 5.3 ) days with
temsirolimus ( n = 204 ) and 75.7 (
6.3 ) days with
IFN - alfa ( n = 196 ) . -
pubmed
In group 2 , however , there was a significant decrease in
post - PCNL
99m - Tc DMSA uptake 2 ( -2.4 + -0.3 % , from 50.1 + -13.5 % to 47.7 + -13.8 % ; P=0.001 ; 95 % CI : 1.13 - 3.66 ) . -
pubmed
As a regulator of
PD ,
PDLP5 was also essential for conferring enhanced innate immunity against bacterial pathogens in a
salicylic acid - dependent manner . -
pubmed
This effect was almost completely abolished by
glibenclamide , a blocker of the
ATP - sensitive potassium channel , but the cardioprotective activity of
SRG was not influenced by the
phosphatidylinositol 3 - kinase (
PI3 K ) inhibitor
LY294002 . -
pubmed
SRG also significantly reduced the
Na ( 2 ) S ( 2 ) O ( 4 )-induced increase in intracellular
calcium , as shown by
Fluo-3 /
AM probes with flow cytometry . -
pubmed
Moreover ,
SRG significantly reduced the level of cardiac
Troponin I (
cTnI ) in the serum , which suggests that
cTnI , a protein component of the
troponin regulatory complex involved in cardiac contractility , contributes to the
SRG - mediated recovery of cardiac systolic function . -
pubmed
SRG significantly attenuated
myocardial ischemic injury by improving cardiac systole function , partly by reducing
cTnI secretion and improving cardiac diastolic function . -
pubmed
Also ,
SRG attenuated the
Ca ( 2 + ) overload in cardiomyocytes and modulated the K(
ATP ) , but not
PI3 K , signaling pathway ; taken together , these mechanisms synergistically reduced
infarct size . -
pubmed
In order to investigate
titanium surfaces , SEM ,
AFM and EDS analyses were carried out . -
pubmed
Transmission Electron Microscopy (
TEM ) was utilized for
Al / CNT agglomerated indicating that the ink paper based on nanocomposite shows good performance as a thermo - piezoresistive sensor . -
pubmed
We segmented the anatomical regions of six different muscles from the lower leg , namely the gastrocnemius [ lateral (
GL ) and medial ( GM ) ] , the tibialis [ anterior ( TA ) and posterior (
TP ) ] , the soleus ( S ) and the peroneus ( P ) and measured the local
PCr resynthesis rate constants . -
pubmed
During the same examination , we also acquired unlocalized ( 31P -
MRS data at a temporal resolution of 6 s. At 3 T , the
PCr resynthesis rate constants were measured at 25.4 + - 3.7 s [ n = 4 , mean + - standard deviation ( SD ) ] using the
MRS method and 25.6 + - 4.4 s using the MRI method . -
pubmed
The successful implementation of our 3D - method suggests that imaging is possible at both fields with a relatively high spatial resolution ( voxel size : 4.2 mL at 3 T and 1.6 mL at 7 T ) using volume
coils and that local
PCr resynthesis rates can be obtained in a single measurement . -
pubmed
Maternal and foetal
triglycerides , total
cholesterol ,
LDL cholesterol and
HDL cholesterol were measured . -
pubmed
Tyr(52 ) to
Trp ) enthalpically contributed for overcoming the energetic barrier to the antigen - antibody interaction , which was a major hurdle for the in vitro affinity maturation of
h528 . -
pubmed
Dystrophin - positive cardiomyocytes and increased proliferation of endogenous
Nestin(+ ) cardiac stem cells were detected in
ADM - injected heart . -
pubmed
No
dystrophin protein , increase in
CD31 expression , or increase in
Nestin(+ ) cell proliferation was detected following
ADM injection in aged mdx heart . -
pubmed
Ribulose-1,5 - bisphosphate carboxylase / oxygenase (
RuBisCO ) is the most abundant protein on the planet and in addition to its central role in photosynthesis it is thought to function as a
nitrogen ( N)-storage protein and a potential source of N for defense biosynthesis in plants . -
pubmed
The decrease of CSAR caused by IMD was prevented by pretreatment with
AM receptor antagonist
AM22 - 52 , but not
CGRP receptor antagonist
CGRP8 - 37 . -
pubmed
PVN
superoxide anions involve in the effect of
IMD on attenuating
Ang II - induced
CSAR response . -
pubmed
Schistosome tegumental ecto - apyrase (
SmATPDase1 ) degrades exogenous pro - inflammatory and pro - thrombotic
nucleotides . -
pubmed
We find that only
SmATPDase1 - suppressed parasites are significantly impaired in their ability to degrade these
nucleotides . -
pubmed
The enzyme is a true
apyrase ;
SmATPDase1 degrades
ATP and
ADP in a cation dependent manner . -
pubmed
The Km of
SmATPDase1 for
ATP is 0.4 + - 0.02 mM and for
ADP , 0.252 + - 0.02 mM. -
pubmed
The results confirm the role of tegumental
SmATPDase1 in the degradation of the exogenous pro - inflammatory and pro - thrombotic
nucleotides ATP and
ADP by live intravascular stages of the parasite . -
pubmed
ZNF165 protein expression was demonstrated on archival
formalin - fixed , paraffin - embedded ( FFPE ) bladder tissues using IHC and nuclear staining pattern was detected . -
pubmed
These laboratories have analysed during the 3 cycles , 2 levels ( medium and high ) of serum concentration of
urea ,
glucose ,
creatinine and
serum aminotransferases . -
pubmed
Angiotensin - converting enzyme ( ACE ) inhibitors and
angiotensin II ( AT II ) receptor blockers ( ARBs ) are widely used antihypertensives with well - recognized renoprotective and cardioprotective effects . -
pubmed
Highly efficient usage of the hydrothermal technique through the one -
pot method to construct four
Keggin - based compounds containing pendent ligands . -
pubmed
Ginsenoside Ro ( Ro ) , an
oleanolic acid - type
ginsenoside , exhibited suppressive activities on reactive
oxygen species ( ROS ) and
matrix metalloproteinase-2 (
MMP-2 ) elevation in UV - B - irradiated fibroblasts . -
pubmed
Perfusion variables included
PI , arterial
lactate level , central venous
oxygen saturation , and the difference between central venous
carbon dioxide and arterial
carbon dioxide pressures , and were recorded before resuscitation and 6 h thereafter . -
pubmed
Influence of
weight reduction on blood levels of
C - reactive protein ,
tumor necrosis factor - alpha ,
interleukin-6 , and
oxylipins in obese subjects . -
pubmed
Plasma Levels of free ( unesterified )
hydroxy ,
epoxy , and
dihydroxy FAs as well as several
prostanoids were analyzed in plasma by means of LC -
MS - based targeted metabolomics . -
pubmed
For most
oxylipins no differences were found between the low and high
hsCRP groups before and after
weight reduction . -
pubmed
OBJECTIVE : To evaluate the safety and efficacy of the oral selective
JAK1 inhibitor
INCB039110 in stable ,
chronic plaque psoriasis . -
pubmed
Therefore we compared the predictive value of the neonatal ( test I ) versus the 48 - h
BH4 loading test ( test
II ) and long - term
BH4 responsiveness . -
pubmed
Hemoglobin ,
xanthine oxidoreductase and
carbonic anhydrase (
CA ) have been reported to convert
nitrite to
NO . -
pubmed
The chemical reaction of
NO2 (- ) with
CO2 and its modulation by
CA isoforms may play important roles in the transport of
nitrite in red blood cells , the kidney and other cells and organs . -
pubmed
Two genes (
pta and
poxB ) in the competitive pathways were deleted from this microorganism to inhibit
pyruvate consumption . -
pubmed
RESULTS : The levels of total
cholesterol (
TC ) ,
high - density lipoprotein cholesterol (
HDL - C ) ,
low - density lipoprotein cholesterol (
LDL - C ) ,
tumor necrosis factor (
TNF ) ,
interleukin-6 (
IL - 6 ) , high - sensitivity
C - reactive protein (
hs - CRP ) and left atrial diameter ( LAD ) all differed significantly between the two groups ( P<0.05 ) . -
pubmed
The kidneys were used for lipid peroxidation (
LP ) and reactive
oxygen species ( ROS ) quantifications . -
pubmed
The canola oil - supplemented diet decreased plasmatic concentrations of free
fatty acids ,
triglycerides , and
cholesterol ; showed improved osmolarity , water clearances , and
creatinine depuration ; and had decreased
LP and ROS . -
pubmed
We found that both the
CpG /
GC dense DNA content and the repetitive nature of
DUX4p arrays are important for their repressive ability . -
pubmed
Using standardized scores for
SCZ and
BPD scaled to a unit increase doubling the risk of the corresponding disorder , the odds ratios for
alcohol and
substance use disorders range from 1.19 to 1.31 for the
SCZ -
PRS , and from 1.07 to 1.29 for the BPD -
PRS . -
pubmed
In multivariate linear regression analysis ,
diastolic blood pressure ( beta = -0.129 , P = 0.024 ) and
high - density lipoprotein cholesterol (
HDL - C ) ( beta = 0.127 , P = 0.036 ) were related to serum
RNase - L. -
pubmed
PN regimens are contaminated with
Mn in amounts likely to meet requirements . -
pubmed
Consequently , it is debated whether
PN should be routinely supplemented with
Mn . -
pubmed
The metabolic flux to
acetic acid in
phosphoketolase pathway was re - directed to
pentose phosphate pathway by substituting the endogenous
phosphoketolase gene (
pkt ) with the heterologous
transketolase (
tkt ) and
transaldolase (
tal ) genes . -
pubmed
BACKGROUND /
AIM : We have previously reported that
caffeine (
CAF ) can enhance chemotherapy efficacy of
bone and soft - tissue sarcoma established cell lines via cell - cycle perturbation . -
pubmed
We subsequently tested the combination of
valproic acid (
VPA ) , a
histone deacetylase (
HDAC ) inhibitor , with
caffeine on established
human osteosarcoma cells in vitro . -
pubmed
Dominance of the hypothalamus - pituitary - adrenal axis over the
renin -
angiotensin -
aldosterone system is a risk factor for decreased
insulin secretion . -
pubmed
How the association between the hypothalamus - pituitary - adrenal (
HPA ) axis and the
renin -
angiotensin -
aldosterone system ( RAAS ) affects
glucose metabolism were not well examined in a general population . -
pubmed
Principal component ( PC ) analysis identified two
PCs :
PC1 represented levels of the
HPA axis ( serum
cortisol ) and the RAAS ( plasma
aldosterone ) as a whole , and PC2 represented the
HPA axis relative to the RAAS (
HPA axis dominance ) . -
pubmed
We examined the association between these PCs and
glucose metabolism using homeostasis model assessment indices of reduced
insulin sensitivity ( HOMA - R ) and secretion (
HOMA - beta ) . -
pubmed
AIM : To compare the efficacy and safety of a single loading low dose
MgSo4 regimen with the Pritchard 's regimen . -
pubmed
Iodinated
CT contrast agents are likely safer than
gadolinium - based MRI contrast agents because of
gadolinium accumulation in the amniotic fluid and fetal tissue , although no harmful effects of tissue
gadolinium accumulation are known . -
pubmed
We previously demonstrated an active
guanylate cyclase (
GC ) catalytic center in the
brassinosteroid insensitive receptor (
AtBRI1 ) within an active intracellular kinase domain resulting in dual enzymatic activity . -
pubmed
This may be through a
cGMP -
AtBRI1 complex forming that may induce a negative feedback mechanism leading to desensitisation of the receptor , regulated through the
cGMP production pathway . -
pubmed
We further argue that the comparatively low but highly localized
cGMP generated by the
GC in response to a ligand is sufficient to modulate the
kinase activity . -
pubmed
MALDI -
MS analysis along with NMR spectroscopic studies of a model reaction of
hydroxy - phenanthriplatin with
acetate establish the formation of stable covalent adducts . -
pubmed
Electron transfer dissociation
MS /
MS fragmentation , a technique that can prevent mass - condition - vulnerable modification of proteins , reveals that
Glu97 preferentially participates over
Glu106 in covalent bond formation to the
platinum center . -
pubmed
The Fc conformation of the
FcRn - binding sites was similar for wild - type and all mutants of hu3S193 Fc , which suggests that
FcRn interactions were directly affected by the
amino acid substitutions . -
pubmed
The analyte and
tramadol(IS ) were extracted from plasma by
protein precipitation with
methanol , and chromatographied on a XDB C(18)(50 mm x 4.6 mm , 1.8 mum ) column using a mobile phase of
methanol and 5 mmol L(-1 )
ammonium acetate(0.01 %
ammonia ) . -
pubmed
HPQ - PDG is able to detect
GGT activity with high spatial resolution and good signal - stability . -
pubmed
In this study , identification and preparation of two recombinant
sulfatases able to catalyze the cleavage of
sulfate groups from fragments of fucoidan molecules is described for the first time . -
pubmed
Detailed specificity and catalytic features of
sulfatases were determined using various
sulfated fucooligosaccharides . -
pubmed
Development of
CDX-1140 , an agonist
CD40 antibody for
cancer immunotherapy . -
pubmed
CDX-1140 binds outside of the
CD40L binding site , and addition of recombinant
CD40L greatly enhances DC and B activation by
CDX-1140 , suggesting that
CDX-1140 may act synergistically with naturally expressed
CD40L. -
pubmed
Our outcomes of interest were daily intakes of
vitamin C ,
magnesium , dietary fiber , and
potassium as estimated by the Block Fruit - Vegetable - Fiber Screener and
kidney disease defined as estimated
glomerular filtration rate & lt;60 mL / min per 1.73 m2 or urinary
albumin - to -
creatinine ratio & gt;=30 mg / g. SETTING AND SUBJECTS : Baseline data from the Achieving Blood Pressure Control Together study , a randomized controlled trial of 159 African Americans ( 117 females ) with uncontrolled
hypertension , were obtained . -
pubmed
MAIN OUTCOME MEASURES : Our outcomes of interest were daily intakes of
vitamin C ,
magnesium , dietary fiber , and
potassium as estimated by the Block Fruit - Vegetable - Fiber Screener and
kidney disease defined as estimated
glomerular filtration rate & lt;60 mL / min per 1.73 m2 or urinary
albumin - to -
creatinine ratio & gt;=30 mg / g. RESULTS : Overall , compared to Institute of Medicine recommendations , participants had lower intakes of
magnesium , fiber , and
potassium but higher
vitamin C intakes . -
pubmed
Below - median intake was significantly associated with
albumin - to -
creatinine ratio & gt;=30 ( adjusted odds ratio [ 95 % confidence interval ] : 3.4 [ 1.5 , 7.8 ] for
vitamin C ; 3.6 [ 1.6 , 8.4 ] for
magnesium ; 2.9 [ 1.3 , 6.5 ] for fiber ; 3.6 [ 1.6 , 8.4 ] for
potassium ) , but not with estimated
glomerular filtration rate & lt;60 . -
pubmed
After positive poling , the depolarization field across the whole volume of
BFO film drove electrons into the
electrolyte solution , inducing a significant anodic shift , Vop of 1.01 V vs.
RHE , together with a significantly enhanced photocurrent density of -91 muA / cm2 at 0 V vs.
RHE under 100 mW / cm2 illumination . -
pubmed
Herein , an integrated Photocatalytic Chitosan -
La3 + - Graphite composite adsorbent was synthesized and the resultant composites were characterized by FTIR , XRD ,
TGA , EDX and SEM analysis . -
pubmed
Arginine deiminase (
ADI ) is a therapeutic protein for
cancer therapy of
arginine - auxotrophic
tumors . -
pubmed
Four
Arg residues on the surface of PpADI
M31 were selected through three criteria and subsequently substituted to
Lys , aiming for providing
primary amines for PEGylation . -
pubmed
The
strain S17 produced 297.85 mg / L of higher
alcohols at 20 C , and the production did not increase at 25 C , reaching about 297.43 mg / L. Metabolite analysis and transcriptome sequencing showed that the metabolic pathways of branched - chain
amino acids ,
pyruvate ,
phenylalanine , and
proline were the decisive factors that affected the formation of higher
alcohols . -
pubmed
The total production of higher
alcohols by the mutants
Deltatir1 and
Deltagap1 was reduced by 23.5 and 19.66 % compared with the parent strain
S17 , respectively . -
pubmed
In this study , thin - layered black
phosphorus ( TLBP ) was used as a unique supporting substrate for the deposition of metal nanoparticles ( MNPs , M =
Pt ,
Ag ,
Au ) , and the resulting M -
TLBP nanocomposites were found to exhibit apparent ORR activity that was readily manipulated by interfacial charge transfer from
TLBP to MNPs . -
pubmed
The ultrasonic sensitivity of a sensor ( T :
EVA and R :
EVA ) significantly enhanced the acoustic impedance in a
methane gas space as pressure increases . -
pubmed
Here , we systematically knocked out TJ components by genome editing and show that while
ZO-1 /
ZO-2 - deficient cells lacked TJ structures and epithelial barriers ,
claudin - deficient cells lacked TJ strands and an
electrolyte permeability barrier but formed membrane appositions and a macromolecule permeability barrier . -
pubmed
Compared with intracavernous ( IC ) injection of
phosphate - buffered saline after
artery injury , of note , we observed that both mesenchymal stem cells ( MSCs ) and
MSC - Exos through IC injection could improve the erectile function to varying degrees . -
pubmed
More specifically , IC injection
MSC - Exos could promote cavernous
sinus endothelial formation , reduce the organization oxidative stress damage , and improve the
nitric oxide synthase and smooth muscle content in the corpus cavernosum . -
pubmed
Selected gene candidates from these groups were verified by qRT - PCR as targets of
EVNorm in
CCl4 - injured livers . -
pubmed
Additionally , EVNorm administration resulted in reduced activation of
p53 , a predicted upstream regulator of 40 % of the genes for which expression was altered by EVNorm following
CCl4 liver injury . -
pubmed
Currently , the available treatment options are very restricted and
colistin - based therapies are last - line treatments of these
infections , even though
colistin resistant (
COLR ) Ab have rarely been isolated yet . -
pubmed
CNP3 was expressed in several endocrine cells :
prolactin - producing cells ( pituitary ) ,
glucagon - producing cells ( pancreas ) , and cardiomyocytes ( heart ) . -
pubmed
We also showed the negative correlation between
CNP3 /
prolactin expression in the pituitary and plasma
Cl- concentration , but not for plasma
Na+ concentration . -
pubmed
In this work , we used
polyacrylamide (
PA ) gels with varying stiffness ( 6.5 - 92.5 kPa ) as the substrate to construct a mechanical microenvironment for cardiomyocytes . -
pubmed
The extensive surface remodeling induced by the deposition of the
apatite - like layer lead to a 3 - fold increase in surface area , a 5 - fold increase in the roughness , and 4 - fold increase in the hardness of the
PBS - immersed scaffolds when compared to the as - printed counterpart . -
pubmed
Hepcidin is a key molecule that regulates
iron metabolism in the body . -
pubmed
The results showed that WBPPS-1 , WBPPS-2 and
WBPPS-3 were composed of
mannose ,
rhamnose ,
galacturonic acid ,
glucose ,
galactose ,
xylose and
arabinose with different ratio and their average molecular weights were 1016.3 , 878.7 and 901.6 KDa , respectively . -
pubmed
An ab initio chemical kinetics study of the reactions of
methyl butanoate (
MB ) with
hydroperoxyl radical (
HO2 ) is presented in this paper . -
pubmed
The transition state theory (
TST ) is used to calculate the high - pressure limit rate constants of the
hydrogen abstraction reactions over a board range of temperature ( 500 - 2000 K ) . -
pubmed
Risk assessment of
osteoradionecrosis associated with
periodontitis using
18F - FDG PET /
CT . -
pubmed
Different with majority of reported hybrid photochromic compounds driven by photochromic active units , the photochromism in 1 and 2 is derived from the electron transfer (
ET ) between
phosphite and non - photochromic
triimidazole - derivative ligand TIPA . -
pubmed
Association of time in range with
hemoglobin A1c ,
glycated albumin , and
1,5 - anhydro - D - glucitol . -
pubmed
AIMS :
Hemoglobin A1c (
HbA1c ) ,
glycated albumin (
GA ) , and
1,5 - anhydro - D - glucitol (
1,5 - AG ) are used as indicators of glycemic control , whereas continuous
glucose monitoring ( CGM ) is used to assess daily
glucose profiles . -
pubmed
RESULTS : In
T1DM and
T2DM , HbA1c and
GA were negatively correlated with TIR , whereas
1,5 - AG was positively correlated with TIR . -
pubmed
The
amino lipid choice ( cationic -
DOTAP and
DDAB ; ionisable -
MC3 ) , buffer choice (
citrate buffer pH 6 or
TRIS pH 7.4 ) and type of nucleic acid payload ( PolyA , ssDNA or mRNA ) have also been shown to have an impact on the characteristics of these LNPs . -
pubmed
Many
APOBEC cytidine deaminase members are known to induce ' off - target '
cytidine deaminations in 5'TC motifs in genomic DNA that contribute to
cancer evolution . -
pubmed
BRET data suggested substantial inhibition of
CYP1A2 - mediated
7 - ethoxyresorufin de - ethylation in the presence of
HO-1 , whereas its activity was actually stimulated at subsaturating
POR . -
pubmed
In contrast ,
HO-1 - mediated
heme metabolism was inhibited at subsaturating
POR . -
pubmed
Regarding
alcohol use , at nine months
post detox , the average units of
alcohol consumption by participants was 18.7 units per week compared to 130.6 units per week before the detox . -
pubmed
Two labeled products were formed ; one was a glycogen - like polysaccharide and the other was a glycoprotein with
glucosyl chains bound to
protein through an acid - labile bond . -
pubmed
After mild treatment of the glucoprotein with acid , the product liberated from the
protein behaved as a mixture of
malto - oligosaccharides and alpha-1,4 - glucan with branches . -
pubmed
The
carbohydrate moiety of the glucoprotein seemed to be released upon prolonged incubation with the
enzyme preparation . -
pubmed
5 - HT antagonists inhibit neuroleptic and
morphine antagonism of the
hyperactivity induced by
DA from the nucleus accumbens [ proceedings ] . -
pubmed
The hydrophobic - rich NH2 - terminal 34
amino acids of a
tetracycline resistance determinant (
TetC ) were fused to the COOH - terminal 240
amino acids of the
hemolysin transporter ,
HlyB , which contains a putative
ATP - binding domain . -
pubmed
Severe
head injury often causes an increase in intracranial pressure ( ICP ) and decreases in cerebral blood flow (
CBF ) and cerebral
oxygen delivery ( CO2del ) . -
pubmed
The role of
carbohydrates in the antigenic properties of
influenza C viral glycoproteins . -
pubmed
The antigenic properties of
influenza C viral glycoprotein gp88 were compared with those of its nonglycosylated counterpart T76 synthesized in infected cells treated with
tunicamycin . -
pubmed
It is unlikely , however , that the antigenic determinant recognized by
Q-5 is
carbohydrate moiety since the ability of the
antibody to bind to
gp88 varied depending on the virus
strain , and
trypsin - treatment of
gp88 eliminated its reactivity with
Q-5 . -
pubmed
Gel electrophoretic analysis under nonreducing conditions showed that T76 underwent the formation of
disulfide - linked multimers in the absence of reducing agent while
gp88 behaved as monomers , suggesting that glycosylation is required for
gp88 molecules to attain an appropriate conformation . -
pubmed
Energy requirements were found to be suppressed , protein metabolism impaired , loss of nitrogenous products increased , fluid and
electrolyte excretion accelerated , vitamin elimination was enhanced ,
corticosteroid blood concentration decreased , and
hemoglobin and cell counts increased . -
pubmed
The limiting factor was found to be the activity of a microsomal
deamidase (
pyrazinoic acid formation from
pyrazinamide and
5 - hydroxy - pyrazinoic acid formation from
5 - hydroxy - pyrazinamide ) . -
pubmed
In contrast , oxidation by
xanthine oxidase occurred very rapidly (
5 - hydroxy - pyrazinamide formation and
pyrazinoic acid catabolism to
5 - hydroxy - pyrazinoic acid ) . -
pubmed
Different factors that give the maximum iodination of
proteins by
iodogen method have been evaluated . -
pubmed
The quantity of
iodogen was varied from 2 to 20 micrograms ; pH from 3 to 9 ; the quantity of
bovine serum albumin (
BSA ) from 25 micrograms to 2 mg ; the 131I activity from 200 microCi to 5 mCi and the reaction time from 2 to 30 min . -
pubmed
The maximum iodination of
proteins is achieved with
iodogen - to -
iodide molar ratio of 100 - 200 and
iodogen - to -
protein molar ratio of 1 - 2 at pH 6 - 8 for 10 - 15 min at room temperature . -
pubmed
Lipid levels did not change , except that
low - density lipoprotein levels decreased from 148 to 127 mg / dl with
prazosin . -
pubmed
Chiral nature of covalent
methylphosphonyl conjugates of
acetylcholinesterase . -
pubmed
This paper examines the chiral nature of the covalent conjugates formed upon reaction of
acetylcholinesterase (
AchE ) with enantiomeric
cycloheptyl ,
isopropyl , and
3,3 - dimethylbutyl methylphosphonyl thiocholines . -
pubmed
Equilibrium titrations with
decidium , a fluorescent bisquaternary competitive inhibitor of
AchE , provided an index of aging and enantiomeric configuration of the conjugates independent of
enzyme activity . -
pubmed
These studies indicate that the active center of
AchE comprises at least two kinetically distinct environments separate from the esteratic region but located within 5 A of the nucleophilic
serine and differing in dipolar characteristics that promote charge separation and general acid catalysis . -
pubmed
Treatment of
prolactin or
bromocriptine failed to induce any change in esterified and free
cholesterol content of the testis . -
pubmed
On the other hand ,
bromocriptine treatment resulted in an increase in the activity of
Mg2 + - ATPase and phospholipid : DNA ratio of the testis with a concomitant decrease in its DNA content . -
pubmed
A reevaluation of the
amino acid sequence of
human follitropin beta - subunit . -
pubmed
Toluene ( 1 g / kg , lh ) did not alter total brain microsomal phospholipid (
PL ) or
cholesterol ( CL ) content . -
pubmed
The
PL / CL ratio , an indirect index of membrane fluidity , did not change , suggesting that
toluene did not affect membrane fluidity . -
pubmed
Dose - response and time - course studies showed that
toluene maximally decreased synaptosomal PL after 1 g / kg , 1 h. The dose - response and time - course studies also showed that the
toluene - induced decreases in
PL were a result of specific decreases in
phosphatidylethanolamine (
PE ) . -
pubmed
Since
PE was decreased , we assessed whether
toluene altered synaptosomal membrane function by investigating phospholipid methylation , a reaction which uses
PE as its initial substrate . -
pubmed
Active site - directed irreversible inhibition of
glutathione S - transferases by the
glutathione conjugate of
tetrachloro-1,4 - benzoquinone . -
pubmed
The derivative bound to the
enzyme still possesses a
quinone structure and is able to react with
thiol - containing compounds . -
pubmed
Reduction of the
enzyme - bound
quinone abolishes its reactivity but does not decrease the inhibition . -
pubmed
At 0 degrees C , the
glutathione conjugate of
tetrachloro-1,4 - benzoquinone inhibits the
glutathione S - transferases at a much higher rate than the corresponding
beta - mercaptoethanol conjugate , indicating a distinct targetting effect of the
glutathione moiety . -
pubmed
However , the parent compound ,
tetrachloro-1,4 - benzoquinone , also has a considerable affinity for the
enzymes . -
pubmed
Tumor necrosis factor augments the immunogenicity and the production of
L - ornithine by peritoneal macrophages . -
pubmed
The release of
ornithine by macrophages and its correlation with their immunogenicity after treatment with
recombinant human tumor necrosis factor - alpha (
TNF ) were analyzed . -
pubmed
TNF was found to augment the capacity of peritoneal macrophages to convert
14C - labeled arginine into
L - ornithine and to release
L - ornithine into the culture medium . -
pubmed
T1 measured in a tubule suspension ( 22 % of exchangeable water in the intracellular compartment ) containing 20 mM extracellular
MnCl2 was biexponential with time constants 1.8 + /- 0.1 ms and 8.3 + /- 0.2 ms ( mean + /- SD , n = 8 , 37 degrees C , 10 MHz ) . -
pubmed
The model predicted that the slow
T1 was relatively insensitive to apical membrane
Pd because of low luminal / cell volume ratio . -
pubmed
Individual cells in heterogeneous populations can be tested for
cell surface antigens using monoclonal antibodies conjugated to
fluorescein - containing microspheres and subsequently stained for
myeloperoxidase ,
Sudan black B ,
chloroacetate esterase , or
alpha naphthyl butyrate . -
pubmed
Collagen content was measured in 38 needle liver biopsies ( 8
steatosis , 8
chronic hepatitis , 7
fibrosis and 15
cirrhosis ) by a new colorimetric method based on the selective capacity of Sirius red and
Fast green to bind to
collagen and noncollagenous proteins , respectively . -
pubmed
Globin from hemoglobin A and
hemes with altered 2,4 - substituents gave reconstituted
hemoglobins that autooxidize at rates that decrease with the electron - withdrawing power of the substituent :
acetyl greater than
vinyl greater than
hydrogen greater than
ethyl . -
pubmed
Acetylcholine release from growth cones detected with patches of
acetylcholine receptor - rich membranes . -
pubmed
To examine the development of
ACh release we have used isolated patches of
acetylcholine receptor(
AChR)-rich membrane as sensitive detectors of
ACh . -
pubmed
Cytochrome P-450 oxidation of
alkanes originating as scission products during lipid peroxidation . -
pubmed
Administration of drugs which inhibit or induce
cytochrome P-450 indicated that a particular
isoenzyme might be involved in the oxidation of small
alkanes . -
pubmed
CCl4 destroys
cytochrome P-450 dose dependently , so that it loses its capacity to oxidize
pentane , whereas
paracetamol does not inactivate the
mono - oxygenase . -
pubmed
Monitoring the elimination rate of injected
pentane is recommended as a reliable non - invasive procedure for testing the functional state of hepatic
cytochrome P-450 . -
pubmed
These findings demonstrate that the formation of GSSQ is catalyzed by a
GSSG specific enzyme which is supposed to be
thioltransferase (
Glutathione : Disulfide Oxidoreductase ) . -
pubmed
A specific inhibitor of
gamma - glutamyltranspeptidase ,
anthglutin , inhibited the hydrolysis . -
pubmed
The most sensitive UV detection of trace amounts of
CT was achieved with
tetrabutylammonium phosphate buffer (
TBAP ) . -
pubmed
We recommend for RP - HPLC of
CT a
C18 - bonded
silica column and elution with a 20 - min linear gradient of
methanol - water ( 20:80 to 80:20 , v / v ) containing 0.005 M
TBAP . -
pubmed
Combined with appropriate extraction procedures , such as
silica adsorption or immuno - adsorbant chromatography , this paired - ion RP - HPLC method can be an important aid in achieving more accurate and extensive information about
CT moieties in biological samples . -
pubmed
Phosphoamino acid analysis of the autophosphorylated
kinase shows that
ADK1 phosphorylates
serine ,
threonine , and
tyrosine . -
pubmed
Using
poly ( Glu / Tyr ) as a substrate , we confirm that
ADK1 is capable of phosphorylating
tyrosine residues . -
pubmed
METHODS AND RESULTS : To evaluate the presence of reinnervation , we measured the power of RR - LF and RR -
HF in 26 heart transplant recipients and 16 control subjects before and during sinusoidal neck suction at 0.1 Hz and 0.20 Hz ( similar to but distinct from that of controlled respiration , 0.25 Hz ) and before and during administration of
atropine or beta - blocker (
esmolol hydrochloride ) by spectral analysis . -
pubmed
The organisation of the cytoskeleton was determined by double fluorescence labeling with antibody anti -
vimentin , anti -
vinculin , anti -
tubulin , and with
rhodamine - labeled
phalloidin . -
pubmed
The only
CpG island found in this region was within the
N - myc gene . -
pubmed
LAZ3 is transcribed as a 3.8 kb
message predominantly in normal
adult skeletal muscle and in several
NHL carrying 3q27
chromosomal defects . -
pubmed
Gossypol (
GP ) , a naturally occurring toxin , inhibits the growth of various
carcinoma cells . -
pubmed
Decreased
[ 3H ] thymidine incorporation was observed in
post - confluent cultures of both cell types relative to pre - confluent counterparts . -
pubmed
Light production was dependent on phage
infection , expression of the
luciferase gene , and the level of cellular
adenosine triphosphate . -
pubmed
Disintegrins , a group of
Arg - Gly - Asp ( RGD)-containing peptides from viper venom , are potential anti - adhesion agents that interfere with
integrin - ECM binding . -
pubmed
The cytotoxic effect of
disintegrins was examined with a cell proliferation assay using the
alamar blue method . -
pubmed
Disintegrins , echistatin , and
flavoridin inhibited
RPE cell attachment to the ECM . -
pubmed
The potency of
disintegrins was 150 to 300 times higher than that of
Gly - Arg - Gly - Asp - Ser ( GRGDS ) peptide . -
pubmed
In the in vivo experiment , echistatin ( 50 microgram / ml ) or
flavoridin ( 80 microgram / ml ) significantly inhibited
RPE cell - induced tractional
retinal detachment compared with the control group at week 2 ( P< ; 0.05 ) and week 4 ( P< ; 0.01 ) after surgery . -
pubmed
We propose that
nedocromil sodium and
cromolyn sodium can affect water transport into and out of the epithelial cell by an action on
chloride ion channels . -
pubmed
GK mRNA was induced by
insulin maximally under venous
O2 partial pressure ( pO2 ) and only half - maximally under arterial pO2 . -
pubmed
CoCl2 and
desferrioxamine mimicked venous pO2 and enhanced the
insulin - dependent induction of
GK mRNA under arterial pO2 . -
pubmed
H2O2 mimicked arterial pO2 and reduced
insulin - induced
GK mRNA under venous pO2 to the lower arterial levels . -
pubmed
Thus the zonal
O2 gradient in liver seems to have a key role in the heterogenous expression of the
GK gene . -
pubmed
Since the presence of the
TNFa6 allele correlates with low
TNF alpha production in response to
lipopolysaccharide , it could be speculated that
DR2 - negative MS patients have an increased risk of being high
TNF alpha producers in response to exogenous stimuli . -
pubmed
Increased expression of
c - jun , but not
retinoic acid receptor beta , is associated with
F9 teratocarcinoma stem cell differentiation induced by
polyamine depletion . -
pubmed
alpha - Difluoromethylornithine (
DFMO ) , an enzyme - activated irreversible inhibitor of
ornithine decarboxylase , and
all - trans - retinoic acid ( RA ) are known to induce
F9 teratocarcinoma stem cell differentiation . -
pubmed
Thus ,
retinoic acid receptor (
RAR ) alpha mRNA is weakly expressed during
DFMO treatment , but strongly induced during an early phase of
RA treatment . -
pubmed
RAR gamma mRNA is relatively strongly expressed in untreated control cells and remains at approximately the same level during
DFMO - induced differentiation . -
pubmed
Hevin , a gene closely related to the extracellular matrix protein
SPARC , is an acidic
cysteine - rich glycoprotein shown to be important for the adhesion and trafficking of cells through the endothelium . -
pubmed
In situ hybridization with an
35S - labeled antisense riboprobe demonstrated the loss of
Hevin expression in
metastatic prostate carcinoma . -
pubmed
Mo accumulated in all organs including brain and pituitary ( P & lt ; 0.02 ) in all
TTM trial groups and was retained after cessation of treatment , except in liver , kidney and skeletal muscle . -
pubmed
Brain
Cu v.
Mo concentrations showed a strongly positive correlation ( r. 0.7 ) in the high -
Cu Ronaldsay group 7 months after
TTM treatment . -
pubmed
In addition
TTM may redistribute some displaced excess liver
Cu (
Cu - TTM ) to the brain . -
pubmed
Activation of postsynaptic
D-1 and D-2 dopamine ( DA ) receptors with
apomorphine administered 30 min before stress attenuated stress - induced immunosuppression as well . -
pubmed
RESULTS : The Vmean and heart rate increased significantly with increasing concentrations of
desflurane ( Vmean from 68 + /- 27 to 106 + /- 30 cm.s-1 and heart rate from 109 + /- 17 to 136 + /- 15 b.min-1 between
T1 and T7 ) . -
pubmed
Calcium ,
phosphate ,
25 - hydroxyvitamin D , and
osteocalcin increased significantly from midpregnancy to weaning whereas
1,25 - dihydroxyvitamin D values showed significant decreases . -
pubmed
The vitamin supplemented lipopolysaccharide ( LPS ) vaccinated group exhibited greater immune ( both humoral and cell mediated ) responses than its
formalin killed ( FK ) and heat killed (
HK ) bacterin vaccinated counterparts . -
pubmed
These actions of
NOHA were abolished in the presence of exogenous
L - ornithine suggesting that a reduction in the intracellular
polyamine content might be responsible for the activation of
caspase-3 and apoptotic actions of
NOHA . -
pubmed
In order to further explore this possibility , we used
SAM-486A and
alpha - difluoromethylornithine (
DFMO ) , which are inhibitors of
S - adenosylmethionine decarboxylase (
SAMDC ) , and
ornithine decarboxylase (
ODC ) , respectively , either alone or in combination to reduce the intracellular
polyamine levels . -
pubmed
We then assessed whether a reduction in
polyamine levels by these two compounds to a similar degree to that produced by
NOHA activated
caspase-3 which occurs prior to the onset of apoptosis . -
pubmed
We observed that both
SAM-486A and
DFMO , either alone or in combination , inhibited cell proliferation , induced
p21 and arrested cells in the G(0)-G(1 ) phase of the cell cycle but failed to activate
caspase-3 as assessed by enzymatic assay of
caspase-3 , western blot analysis of the proteolytic cleavage of
caspase-3 protein as well as TUNEL assay . -
pubmed
Furthermore , pre - incubation of the cells with
SAM-486A and
DFMO for 4 days , either alone or in combination significantly inhibited the activation of
caspase-3 and apoptosis by
NOHA when compared with that observed with cells treated with
NOHA alone . -
pubmed
Our results , therefore , indicate that the activation of
caspase-3 and apoptosis observed with
NOHA can not be solely explained by a reduction in intracellular
polyamine levels and that other mechanisms need to be also considered . -
pubmed
The
HLA class I gene
HFE seemingly no longer participates in immunity because it has lost its ability to bind peptides and it has acquired the ability to form complex with the
receptor for iron - binding transferrin by regulating
iron uptake by intestinal cells . -
pubmed
The usefulness of many
beta - lactams , including the third - generation
cephalosporins , has been threatened in recent years by the dissemination of
beta - lactamase - mediated resistance . -
pubmed
Due to the unique arrangement of the moieties around the
carbapenem beta - lactam ring , these agents possess unrivaled stability to almost all
serine beta - lactamases , including the mutant extended - spectrum types produced by Enterobacteriaceae which hydrolyze third - generation
cephalosporins . -
pubmed
The presence of
CO , however , further increased both pulmonary vascular resistance (
PVR ) and the number of small muscular vessels compared with
HH alone . -
pubmed
CO +
HH also increased vascular
PCNA and nuclear ssDNA expression compared with
hypoxia , suggesting accelerated cell turnover . -
pubmed
CO in
hypoxia downregulated
sm - alpha - actin and strongly upregulated
beta - actin . -
pubmed
CO also increased lung
HO activity and
HO-1 mRNA and protein expression in small pulmonary arteries during
hypoxia . -
pubmed
These data indicate an overall propensity of
CO in
HH to promote
vascular remodeling and increase
PVR in vivo . -
pubmed
As a prodrug
leflunomide is completely converted to its active metabolite
A 77 1726 (
M1 ) which blocks the
dihydroorotate dehydrogenase , a key enzyme of the
pyrimidine de novo synthesis . -
pubmed
This study investigated the contribution of the entorhinal cortex in this reinnervation by comparing the distribution of the entorhinal axons in the
CA1 subfield between the intact hippocampus and the
CA3 - lesioned hippocampus at 3 months after administration of
kainic acid . -
pubmed
However , after
kainic acid - induced
CA3 lesion , the density of entorhinal axons increased significantly in the
CA1 stratum radiatum ( 375 % of the intact hippocampus ) , as a large number of axons emanating from the entorhinal fiber plexus in the stratum lacunosum moleculare invaded the stratum radiatum . -
pubmed
BFA induced a rapid regression of the Golgi stack into rudimentary Golgi clusters composed of tubulovesicules , in parallel with a redistribution of the Golgi - resident proteins and a
coat protein (
beta - COP ) into the region of the rough endoplasmic reticulum ( rER ) or cytosol . -
pubmed
[ Pharmacokinetic / pharmacodynamic analysis of anti -
hyperprolactinemic effect of
terguride based on
dopamine D2 receptor occupancy ] . -
pubmed
Terguride has been widely used for the treatment of
hyperprolactinemia via partial agonistic action on
dopamine D2 receptors in the pituitary . -
pubmed
The present study analyzed retrospectively the
dopamine D2 receptor binding occupancy ( phi ) of
terguride . -
pubmed
The average phi value was estimated to be
14.1 % after oral administration of the average / standard therapeutic dose of
terguride . -
pubmed
Furthermore , we developed a pharmacokinetic / pharmacodynamic model for ascertaining the serum
prolactin - lowering effect of
terguride , considering both the reversible binding to
D2 receptors and the effect on the increase rate in the
prolactin level . -
pubmed
Thus , conditions in which
methanol would not be produced in the headspace -
GC method were investigated . -
pubmed
melanogaster species subgroup , the
histone gene regions as well as 3rd codon position of the coding region showed nearly the same
GC contents . -
pubmed
These results suggested that the previous conclusion on analysis of the
H3 gene regions , the gene family evolution in a concerted fashion , holds true for the whole
histone gene repeating unit . -
pubmed
Pavlovian fear conditioning regulates
Thr286 autophosphorylation of
Ca2 +/ calmodulin - dependent protein kinase II at lateral amygdala synapses . -
pubmed
Next , we demonstrate that intra - amygdala infusion of a
CaMK inhibitor ,
1 -[ NO - bis-1,5 - isoquinolinesulfonyl ]- N - methyl - l - tyrosyl-4 - phenylpiperazine ,
KN-62 , dose - dependently impairs the acquisition , but not the expression , of auditory and contextual fear conditioning . -
pubmed
Finally , in electrophysiological experiments , we demonstrate that an
NMDA receptor - dependent form of long - term potentiation at thalamic input synapses to the LA is impaired by bath application of
KN-62 in vitro . -
pubmed
Reducing the number of treatments to twice a week and reducing the UVA dose from 1
MPD to 0.75 or 0.5
MPD per treatment only slightly affected intermediate therapeutic efficacy ( between the second and seventh weeks of therapy ) but had no effect on final clearance rates . -
pubmed
During
tissue damage ,
S - alk ( en ) yl cysteine sulfoxides are converted to their respective
thiosulfinates or
propanethial - S - oxide by the action of the enzyme
alliinase (
EC 4.4.1.4 ) . -
pubmed
Low - dose
STI571 monotherapy was not effective in
extra - osseous disease , apparently due to a site - specific failure to up - regulate
beta - platelet - derived growth factor receptor expression in
prostate cancer cells and associated
tumour stroma . -
pubmed
Both these effects were blocked by the TK inhibitor
tyrphostin-23 , while the
MAPK p42 /
p44 inhibitor
PD-98059 annulled only
MAPK p42 /
p44 activation . -
pubmed
The growth - stimulating effect of 10(-6 ) M
NPB and
NPW were not affected by either the
PKA and
PKC inhibitors
H-89 and
calphostin - C or the
MAPK p38 antagonist
SB-293580 , but were abolished by both
tyrphostin-23 and
PD-98059 . -
pubmed
Addition of 0.15 g / L
MgSO ( 4 ) was the most favorable for
levansucrase production . -
pubmed
The results indicated that high
enzyme concentrations produced increasing amounts of levan , and hence conversion of
fructose to levan reached 84 % using 1,000 microg / ml
enzyme protein . -
pubmed
A sensitive assay for
lipase using
tetra sulfonatophenyl porphyrin . -
pubmed
The coupling of NSAIDs with a
NO - donating moiety has led to the birth of a new class of anti - inflammatory drugs , called the
COX - inhibiting
nitric oxide donators ( CINODs ) . -
pubmed
Rate constants for reactions of secondary
alkyl radicals with
triethylborane and its complexes with water ,
deuterium oxide ,
methanol , and
THF at ambient temperature were determined by radical
clock methods . -
pubmed
Cyclization of the
1 - undecyl-5 - hexenyl radical and ring opening of the
1 - cyclobutyldodecyl radical were evaluated as
clock reactions . -
pubmed
L and D isomers of
amino acids were employed as inhibitors to determine the stereo - selectivity of
LAT . -
pubmed
RESULTS : Retinal uptake of
L - Phe was significantly inhibited in presence of a specific
LAT inhibitor ,
2 - aminobicyclo -[ 2,2,1 ]- heptane-2 - carboxylic acid (
BCH ) , L isomers of large neutral
amino acids and
LAT1 specific but not by
LAT2 specific and charged
amino acids . -
pubmed
No significant inhibition of
L - Ala retinal uptake was observed with
LAT substrates . -
pubmed
The mean residence time ( MRT ) and area under curve ( AUC ) values of
L - Phe following intravitreal administration were significantly increased in the presence of
D - methionine , a
LAT substrate . -
pubmed
Furthermore , based on these studies it can be concluded that
LAT is involved in the retinal uptake and intravitreal elimination of
L - Phe . -
pubmed
Serum proprotein convertase subtilisin kexin type 9 is correlated directly with serum LDL
cholesterol . -
pubmed
BACKGROUND :
Proprotein convertase subtilisin kexin type 9 (
PCSK9 ) is gaining attention as a key regulator of serum LDL -
cholesterol (
LDLC ) . -
pubmed
RESULTS : Serum concentrations of
PCSK9 ranged from 11 to 115 microg / L and were directly correlated with serum concentrations of
LDLC ( r = 0.45 , P = 0.001 ) and total
cholesterol ( r = 0.50 , P = 0.0003 ) , but not with
triglycerides ( r = 0.15 , P = 0.28 ) or
HDL cholesterol concentrations ( r = 0.13 , P = 0.36 ) . -
pubmed
Forced expression of the
alpha7 integrin or use of the
mitogen - activated protein kinase kinase 1 inhibitor
U0126 in
alpha7 integrin - deficient VSMCs suppressed
extracellular signal - regulated kinase activation and restored the differentiated phenotype to
alpha7 integrin - null cells in a manner dependent on
Ras signaling . -
pubmed
2 - Methoxyestradiol (
2 - ME ) is an endogenous metabolite of
estradiol with no affinity for
estrogen receptors . -
pubmed
The goal of this round - robin study is to investigate the capability of a novel solid - phase microextraction - gas chromatographic ( SPME -
GC ) method for trace - level
EtO residuals analysis : three independent laboratories conducted a guided experiment using this SPME -
GC method , in assessing method performance , ruggedness and the feasibility of SPME fibers . -
pubmed
SPME is a feasible alternative for quantifying trace - level
EtO residuals in sterilized medical devices , thereby lowering the limit of quantification ( LOQ ) by as much as two to three orders of magnitude over the current
GC methodology of direct liquid injection . -
pubmed
Mutations in different codons causing resistance to
isoniazide in the gene of
catalase peroxides (
katG ) in Belarusian
strains was determined . -
pubmed
This indicated that
METH induced an increase in the phosphorylation of
ERK and
JNK , but not of
p38 . -
pubmed
Moreover , a
JNK - specific inhibitor ,
SP600125 , partially but significantly rescued
METH - induced cell
death , while
PD98059 ( an
ERK kinase inhibitor ) and
SB203580 ( a
p38 inhibitor ) had no protective effect . -
pubmed
We also found that
vitamin E (
Vit E ) prevented
METH - induced
JNK phosporylation and
SP600125 inhibited
METH - induced
c - Jun phosphorylation . -
pubmed
Furthermore ,
METH - activated
caspase-3 activity was significantly repressed by
Vit E and in
SP600125 treated cells . -
pubmed
We suggest that the oxidative stress - activated
JNK signaling pathway is involved in
METH - induced cell
death . -
pubmed
Metal selective fluorescent peptide probes (
dansyl - Cys - X - Gly - His - X - Gly - Glu - NH2 , X =
Pro or
Gly ) were developed by synthesizing peptides containing
His ,
Cys , and
Glu residues with
Pro - Gly sequence to stabilize a turn structure and
Gly - Gly sequence to adopt a random
coil . -
pubmed
The probe containing two
Gly - Gly sequences exhibited marked selectivity only for
Cu2 + over 13 metal ions including competitive transition and Group I and
II metal ions under physiological buffer condition . -
pubmed
The purified protein showed a single
protein band and the molecular weight was about 110kDa on
sodium dodecyl sulfate - polyacrylamide gel electrophoresis (
SDS - PAGE ) under reducing conditions . -
pubmed
Vitamin D2 and
ergosterol were measured by HPLC -
MS /
MS . -
pubmed
Early outcomes of
thymoglobulin and
basiliximab induction in kidney transplantation : application of statistical approaches to reduce bias in observational comparisons . -
pubmed
RESULTS : All statistical approaches demonstrated lower rates of the 6 - month triple endpoint with
thymoglobulin compared with
basiliximab when
steroids were present , with approximately 22 % adjusted , relative reduction by logistic regression analysis and 3 % absolute reductions by matching approaches . -
pubmed
CONCLUSIONS : Consistency across statistical approaches suggests superiority of thymoglobulin compared with
basiliximab or no antibody induction therapy for 6 - month kidney transplant outcomes in the modern immunosuppression
era . -
pubmed
Baseline
albuterol use , total daily
asthma score , and nighttime awakenings were maintained after
CIC treatments ( p & gt ; 0.25 ) , but increased after placebo ( p & lt ; or = 0.002 ) ; the difference between CIC80 b.i.d . -
pubmed
An NMR study on
nickel binding sites in
Cap43 protein fragments . -
pubmed
NMR spectroscopy was used to study the interaction of
Ni ( II ) ions with C - terminal sequence of
Cap43 protein where , from
Thr341 to
Gly360 residue , a
T1R2S3R4S5H6T7S8E9G10 ten -
amino acid fragment is consecutively repeated three times . -
pubmed
The behaviour of ends - blocked Ac - RSRSHTSEG - Am (
pept1 ) , Ac - TRSRSHTSEG - Am (
pept2 ) , and the three repeats Ac - TRSRSHTSEG - TRSRSHTSEG - TRSRSHTSEG - Am ( pept3 ) peptides towards
Ni ( II ) ions was examined at different pH values and , for pept3 , at different ligand - to - metal molar ratios . -
pubmed
In the present work four new ,
tyrosine containing HN - derivatives were synthesized ( Y - PAGASRLLLTGEIDLP , peptide I ; Y - PAGASRLLLLTGEIDLP , peptide II ; Y - SALLRSIPAPAGASRLLLTGEIDLP , peptide III ; Y - SALLRSIPAPAGASRLLLLTGEIDLP , peptide
IV ) . -
pubmed
[ Myoanabolic
steroids and selective
androgen receptor modulators : mechanism of action and perspectives ] . -
pubmed
Interest in anabolic
steroids has been renewed in the last decade with the discovery of tissue - selective
androgen receptor modulators exhibiting high myotropic and small androgenic activity . -
pubmed
An explanation put forward by us in 1982 for the mechanism of the preferential myotropic effect of
nandrolone (
19 - nortestosterone ) exploits the fundamental difference between the
5alpha - reductase concentrations in skeletal muscle and androgenic target tissue . -
pubmed
Transcapillary ultrafiltration and fluid absorption rates were assessed using radiolabeled
albumin , and free water transport by kinetics of
sodium concentration in dialysis fluid . -
pubmed
Unexpectedly severe acute radiotherapy side effects are associated with single
nucleotide polymorphisms of the
melanocortin-1 receptor . -
pubmed
Deoxyribonucleic acid sequence variants in the form of single nucleotide polymorphisms ( SNPs ) of
MC1R affect
melanin expression and are linked to skin phenotype . -
pubmed
Diminution of
AHR and
inflammation was associated with reduced
IL-4 ,
IL-5 , and
RANTES production in the
BAL fluid at the 30 microg
ITE dose , while OVA specific
IgE and
eotaxin serum levels remained unchanged . -
pubmed
CONCLUSION :
ITE , which has been reported inhibiting
COX-2 and
5 - LOX , reduced
allergic airway inflammation and
AHR by inhibiting the production of the Th2
cytokines IL-4 and
IL-5 , and
RANTES . -
pubmed
Syndecan-1 - integral membrane
heparan sulfate proteoglycan and
cathepsins D and K - lysosomal proteases are important factors influencing different aspects of these processes . -
pubmed
Microcystins ( MCs ) represent a group of & gt ; 80 cyclic heptapeptides , mediating
cytotoxicity via specific
protein phosphatase (
PP ) inhibition at equimolar concentrations ( comparable toxicodynamics ) . -
pubmed
Further clinical studies evaluating the ideal moment of
fish oil LE infusion to modulate leukocyte functions may contribute to a better understanding of its immune modulatory properties . -
pubmed
Flavopiridol protects against
inflammation by attenuating leukocyte - endothelial interaction via inhibition of
cyclin - dependent kinase 9 . -
pubmed
OBJECTIVE : The
cyclin - dependent kinase (
CDK ) inhibitor
flavopiridol is currently being tested in clinical trials as anticancer drug . -
pubmed
Endothelial cells were found to be the major target of
flavopiridol , which blocked the expression of
endothelial cell adhesion molecules (
intercellular adhesion molecule-1 ,
vascular cell adhesion molecule-1 , and
E - selectin ) , as well as
NF - kappaB - dependent transcription . -
pubmed
Flavopiridol did not affect
inhibitor of kappaB ( IkappaB ) kinase , the degradation and phosphorylation of
IkappaBalpha , nuclear translocation of
p65 , or
nuclear factor - kappaB (
NF - kappaB ) DNA - binding activity . -
pubmed
By performing a cellular kinome array and a
kinase activity panel , we found
LIM domain kinase-1 (
LIMK1 ) ,
casein kinase 2 ,
c - Jun N - terminal kinase (
JNK ) ,
protein kinase C (
PKC ) ,
CDK4 ,
CDK6 ,
CDK8 , and
CDK9 to be influenced by
flavopiridol . -
pubmed
Using specific inhibitors , as well as RNA interference ( RNAi ) , we revealed that only
CDK9 is responsible for the action of
flavopiridol . -
pubmed
CONCLUSIONS : Our study highlights
flavopiridol as a promising antiinflammatory compound and inhibition of
CDK9 as a novel approach for the treatment of
inflammation - associated diseases . -
pubmed
Serum
estradiol levels increased from 20.9 pg / mL at 0 h to 461 pg / mL at 48 h. Northern analysis was performed for analysis of
TIMP-1 ,
TIMP-2 , and
TIMP-3 expression ( N = 4 ) . -
pubmed
Continuum source
tungsten coil atomic fluorescence spectrometry . -
pubmed
A simple continuum source
tungsten coil atomic fluorescence spectrometer is constructed and evaluated . -
pubmed
A 25 muL sample aliquot is pipetted onto the
tungsten coil , the liquid is dried at low current , and then the atomic vapor is produced by applying a current in the range 3.5 - 5.5 A. -
pubmed
Orally - administered
prostanoid DP receptor antagonists
S-5751 and
pinagladin significantly suppressed not only
bronchial hyperresponsiveness and
lung eosinophilia but also
neutrophilia and mucus secretion in the lung , while oral
prednisolone inhibited only
bronchial hyperresponsiveness and eosinophil infiltration . -
pubmed
In addition ,
prostanoid DP receptor antagonists significantly suppressed interleukin ( IL)-1beta ,
IL-6 and
CXCL1 mRNA in contrast to suppression of
IL-4 and CCL11 mRNA by
prednisolone . -
pubmed
Batch and repeated batch cultivation using
calcium alginate immobilized cells were studied for
alkaline protease production in submerged fermentation . -
pubmed
During the long development of
hemoglobin (
Hb)-based
O ( 2 ) carriers , many side effects of cell - free
Hb molecules have arisen , and have implied the physiological importance of the cellular structure of red blood cells ( RBCs ) . -
pubmed
However , following rumen biohydrogenations , these
FAs could still be metabolised in various tissues / organs involved in the
FA metabolism such as the liver and adipose tissues ( ATs ) , thus affecting their subsequent deposition in muscles . -
pubmed
Hepatic secretion of
ALA as part of the
very - low - density lipoprotein particles was lower than that of LA ( -58 % and -23 % for C and LR diets respectively , P = 0.02 ) . -
pubmed
They were efficiently taken up by
ATs ( & gt;68 % of
FA present in the medium ) , with uptake being higher for IM than for SC
AT ( +12 % , P = 0.01 ) . -
pubmed
Both
FAs were mainly esterified ( & gt;97 % of
FA present in adipocytes ) into neutral lipids ( & gt;85 % of
esterified FA ) . -
pubmed
Glucose -
aspirin (
GA ) was synthesized by conjugating
aspirin (
ASA ) to
glucose . -
pubmed
The water solubility and biological activity of
GA was studied in comparison to
aspirin . -
pubmed
The
human serum protease activity on the
ester showed a slower hydrolysis rate , compared to
ASA . -
pubmed
In conclusion ,
GA is a highly water soluble derivative of
aspirin . -
pubmed
Stable
Smad7 overexpression significantly inhibited the absorbance in the
MTT - dye assay and inhibited the levels of
PCNA compared with those in empty vector - transfected cells . -
pubmed
Endogenous over - production of the required precursor ,
lysine , was first achieved through metabolic deregulation of its biosynthesis pathway by introducing feedback resistant mutants of
aspartate kinase III and
dihydrodipicolinate synthase . -
pubmed
Further disruption of native
lysine decarboxylase activity ( by deleting
cadA and
ldcC ) limited
cadaverine by - product formation , enabling
lysine production to 2.25 g / L at a
glucose yield of 138 mmol / mol ( 18 % of theoretical ) . -
pubmed
Co - expression of
lysine monooxygenase and
5 - aminovaleramide amidohydrolase ( encoded by
davBA ) then resulted in the production of 0.86 g / L AMV in 48 h. Finally , the additional co - expression of
glutaric semialdehyde dehydrogenase and
5 - aminovalerate aminotransferase ( encoded by
davDT ) led to the production of 0.82 g / L
glutarate under the same conditions . -
pubmed
A SCOS fabricated with
potassium titanyl phosphate crystal as the slab waveguide is shown to have resonance tuning ranges of 6.67 and 22.24 nm , respectively , for
TM and TE polarized modes . -
pubmed
Their structures were elucidated as
( 25R )- 26 - O - beta - d - glucopyranosyl - furost-5 - ene-12 - one-3beta,22alpha,26 - triol-3 - O - beta - d - glucopyranosyl -( 1 2 )-[ beta - d - xylopyranosyl -( 1 3 )]- beta - d - glucopyranosyl -( 1 4 )- beta - d - galactopyranoside ( 1 ,
typaspidoside A ) ,
( 25S )- 26 - O - beta - d - glucopyranosyl - furost-5 - ene-12 - one-3beta,22alpha,26 - triol-3 - O - beta - d - glucopyranosyl -( 1 2 )-[ beta - d - xylopyranosyl -( 1 3 )]- beta - d - glucopyranosyl -( 1 4 )- beta - d - galactopyranoside (
2 , 25S - typaspidoside A ) , and
timosaponin H1 ( 3 ) , based on the integrative spectroscopic analysis of 1D- and 2D - NMR experiments , ESI -
MS data and chemical evidence . -
pubmed
To get a vivid description of the microenvironment , we added
hydrogen - bond - donor (
HBD ) as well as non -
hydrogen - bond - donor ( NBD ) core solvents . -
pubmed
For experimental purposes , we used water ,
ethylene glycol ( EG ) ,
glycerol ( GY ) as
HBD solvents and
N , N - dimethyl formamide (
DMF ) as a NBD solvent . -
pubmed
With increasing amount of core solvents , irrespective of
HBD or
NBD , the fluorescence intensity and lifetime of
curcumin increase with remarkable red - shift inside the reverse micelle . -
pubmed
RESULTS : Immunohistochemistry analysis showed significant increase of
GSK-3beta in the cerebral cortex of 7 - month - old
APP group ( compare to control group P = 0.003 ) , while the
GSK-3beta expression of
donepezil or GEPT group were all significantly decreased (
Donepezil vs
APP : P = 0.041 ; GI vs
APP : P = 0.049 ; Gm vs
APP : P = 0.029 ; Gh vs
APP : P = 0.036 ) . -
pubmed
And the
GSK-3beta expression in
donepezil and GEPT groups were all decreased . -
pubmed
Short - term
cuprizone exposure resulted in a selective decrease of
alanine ,
glycine , and
proline plasma levels , which was paralleled by an increase of apoptotic cells in the liver and a decrease of
alanine aminotransferase in the serum . -
pubmed
Our results suggest that
cuprizone - induced
liver dysfunction results in
amino acid starvation and in consequence to the activation of an
AAR . -
pubmed
Furthermore , the membrane
toxicity of all applied
saponins was analyzed using extracellular
LDH quantification and the general
cytotoxicity was analyzed using a colorimetric
MTT - assay and DNA quantification . -
pubmed
The sampled particles were extracted from the
polyurethane foam (
PUF ) impaction substrates using a previously developed and validated protocol , and used in a cellular assay to assess oxidative stress . -
pubmed
Attenuation of skeletal muscle and
renal injury to the lower limb following
ischemia - reperfusion using
mPTP inhibitor
NIM-811 . -
pubmed
Renal
peroxynitrite concentration , serum
TNF - alpha and
IL-6 levels were measured . -
pubmed
Serum K+ and necroenzyme levels were significantly lower in the
NIM - IR group than in the IR group (
LDH : p<0.001 ;
CK : p<0.001 ; K+ : p = 0.017 ) . -
pubmed
Serum
TNF - alpha and
IL-6 levels were significantly lower (
TNF - alpha : p = 0.003 ,
IL-6 : p = 0.040 ) as well as W / D ratio and
peroxynitrite concentration were significantly lower ( p = 0.014 ; p<0.001 ) in the
NIM - IR group than in the IR group . -
pubmed
The industrialisation of lignocellulose conversion is impeded by expensive
cellulase enzymes required for saccharification in
bioethanol production . -
pubmed
Addition of surfactant ,
Tween 80 and metal ion
Mn (+ 2 ) , significantly enhanced
cellulase yield . -
pubmed
Here , we describe the implementation of a picosecond time - resolved X - ray photoelectron spectroscopy ( TRXPS ) technique at the Advanced Light Source (
ALS ) and its application to monitor photoinduced electron dynamics at the technologically pertinent interface formed by
N3 dye molecules anchored to nanoporous
ZnO. -
pubmed
In the present study , a microporous
silicone rubber membrane bilayer (
SRM - B ) composed of two layers with different pore sizes was prepared . -
pubmed
Morphological control revealed a 70 % and 35 % decreases in the amount of
tyrosine hydroxylase positive neurons in substancia nigra / pars compacta after 48 and 24 mg / kg of
MPTP , respectively , as compared to the saline group . -
pubmed
Impact of
estrogen and
progesterone receptor expression on the clinical and molecular features of
papillary thyroid cancer . -
pubmed
INTRODUCTION :
Maraviroc is a first - in - class antiretroviral ( ARV ) drug acting on a host cell target (
CCR5 ) , which blocks the entry of the HIV virus into the cell . -
pubmed
AREAS COVERED : This drug discovery case history focuses on the key studies that led to the discovery and approval of
maraviroc , as well as on
post - launch clinical reports . -
pubmed
The
CCR5 - antagonist
mariviroc , with its unique mode of action and excellent safety profile , is an important therapeutic option for
HIV patients . -
pubmed
In this study , we used
kinase profiling analysis to demonstrate that
2 '- hydroxycinnamicaldehyde (
2 '- HCA ) , a compound found in cinnamon , specifically inhibits
Pim-1 activity . -
pubmed
Cocrystallography studies determined the
hydrogen bonding pattern between 2'-HCA and
Pim-1 . -
pubmed
Notably ,
2 '- HCA binding altered the
apo kinase structure in a manner that shielded the ligand from solvent , thereby acting as a gatekeeper loop . -
pubmed
Overall , our results offered preclinical proof of concept for
2 '- HCA as a potent anticancer principle arising from direct targeting of the
Pim-1 kinase . -
pubmed
CONCLUSION : Uncomplicated soft - tissue
wounds caused by an automatic M70AB2 rifle may be closed primarily with delayed suture without the risk of developing complications if on revision , 24 h after primary surgery , there were no present
necrotic tissues ,
hematoma , and any signs of
infection when
fibrin glue with antibiotics (
ceftriaxone and
clindamycin ) was applied . -
pubmed
However , the
Cd concentrations reached up to 2.5905 , 3.1628 , and 4.8593 mg / kg in the
protein isolates , respectively . -
pubmed
Therefore , 10 common acids in food industry were investigated to remove
Cd from
protein isolate by washing process . -
pubmed
Polo - like kinase 1 (
PLK1 ) is a
serine /
threonine kinase that plays multiple and essential roles during the cell division cycle . -
pubmed
SIRT2 activates
G6PD to enhance
NADPH production and promote
leukaemia cell proliferation . -
pubmed
Knockdown of
G6PD reduces
NADPH level in
acute myeloid leukaemia (
AML ) cell lines . -
pubmed
Deacetylase
SIRT2 promotes
NADPH production through deacetylating
G6PD at
lysine 403 ( K403 ) . -
pubmed
Influence of filtration velocity on
DON variation in
BAF for micropolluted surface water treatment . -
pubmed
Therefore , to control
DON in
BAF effluent , it is necessary to study the influence of
BAF operation parameters on
DON production . -
pubmed
Although plasma polymerization is traditionally considered as a substrate - independent process , we present evidence that the propensity of a substrate to form
carbide bonds regulates the growth mechanisms of plasma polymer (
PP ) films . -
pubmed
Zirconium ,
titanium , and
silicon substrates were used to study the early stages of
PP film formation from a mixture of
acetylene ,
nitrogen , and
argon precursor gases . -
pubmed
On the contrary , the lower propensity of the
silicon surface to form
carbides leads to a 3D , island - like ( Volmer - Weber ) growth mode that creates a sponge - like interphase near the substrate , resulting in inferior adhesion and poor film stability in
SBF . -
pubmed
[
18F - FDG PET /
CT findings of primary intestinal
lymphoma : analysis of 23 cases ] . -
pubmed
CONCLUSION :
PIL presents with characteristic imaging features in
18F - FDG PET /
CT . -
pubmed
18F - FDG PET /
CT is a sensitive imaging modality for detecting inter- and extra - intestinal
lesions of
PIL and displays characteristic imaging features of the disease . -
pubmed
Expression of the
NtEXGT gene in leaves was induced by cytokinins ,
auxins ,
brassinosteroids and
gibberellins . -
pubmed
NtEXGT gene was also up - regulated by salinity , drought , cold , cadmium and 10 muM
abscisic acid treatments and down - regulated in response to 0 C and 100 muM
abscisic acid . -
pubmed
Pretreatment of leaves with
fluridone contributed to smaller increase in the level of
NtEXGT transcripts in response to drought stress . -
pubmed
These data suggest that
NtEXGT gene is
ABA - regulated and probably implicated in
ABA - dependent signaling in response to stress factors . -
pubmed
These results support a novel connection between
HIF-1alpha and
Pak4 in hypoxic
cancer cells , and provide insights into mechanisms whereby
tumors respond to and thrive under
oxygen - deficient conditions . -
pubmed
We show that after a
tumor antigen contact the
IL-2 activated
CD8 + lymphocytes acquire ability to lyse
tumor cells bearing this
antigen . -
pubmed
However , activation of the
CD8 + lymphocytes in the absence of
antigen causes appearance of a cytotoxic population of
CD8 +
NKG2D+ lymphocytes , which are able to lyse HLA - negative
cancer cells that have lost the classic mechanism of
antigen presentation . -
pubmed
Increased
type 2 diabetes risk in
HFE hemochromatosis is associated with one or more factors , including abnormal
iron homeostasis and
iron overload , decreased
insulin secretion ,
cirrhosis ,
diabetes in first - degree relatives , increased
body mass index ,
insulin resistance , and
metabolic syndrome . -
pubmed
In p. C282Y homozygotes , serum
ferritin , usually elevated at
hemochromatosis diagnosis , largely reflects body
iron stores but not
diabetes risk . -
pubmed
Routine
iron phenotyping or
HFE genotyping of patients with
type 2 diabetes is not recommended . -
pubmed
These results suggest that
paraquat degradation can be attributed to the presence of extracellular
enzymes from white rot fungi . -
pubmed
PURPOSE OF REVIEW : It is widely accepted that
sugar sweetened beverages (
SSB ) are implicated in
weight gain and adverse cardiometabolic heath . -
pubmed
Findings from short - term trials of
SSB and markers of cardiometabolic risk including lipids ,
glucose , blood pressure , and inflammatory
cytokines provide mechanistic support for associations with
T2D and
CHD . -
pubmed
These include anterior segment photography , specular microscopy , in vivo confocal microscopy , anterior segment optical coherence tomography (
AS - OCT ) , ultrasound biomicroscopy ( UBM ) , laser flare photometry , and less commonly used techniques such as anterior segment
fluorescein and
indocyanine green angiography , iris autofluorescence , and infrared imaging . -
pubmed
Recombinant
OsPKS2 catalyzed the condensation of
fatty acyl - CoA with
malonyl - CoA to generate
triketide and
tetraketide alpha - pyrones , the main components of pollen exine . -
pubmed
However , unlike
pksb /
lap5 , in which most detected
phenolics were substantially decreased ,
ospks2 accumulated higher levels of
phenolics . -
pubmed
Furthermore , the study also permitted the identification of ten novel prospective allosteric sites named
NP1 to
NP10 , involving in most of the cases protein structural elements that control
kinase activation including the activation loop , the catalytic loop , the alphaC helix , the
L16 loop , and the
glycine - rich loop . -
pubmed
Dual expression of constitutively active
Galphas - protein - coupled receptors differentially establishes the resting activity of the
cAMP - gated
HCN2 channel in a single compartment . -
pubmed
Therefore , some proportion of
HCN2 channels opens in response to the basal
cAMP pool size produced by constitutively active
GPCRs . -
pubmed
Thus , we conclude that the dual expression of constitutively active
GPCRs establishes the diverse range of the basal
cAMP pool size in resting cells through mutual additive or suppressive interactions , even in the absence of external stimulation . -
pubmed
Outer retinal reflectivity was measured on volumetric spectral - domain ( SD)-OCT scans acquired 3 months after
DME resolution , from the photoreceptor ellipsoid zone ( EZ ) and the retinal pigment epithelium (
RPE ) . -
pubmed
Each 1 ng / ml decrease in
sMMP-9 after
NAC was shown to result in a 0.3 % increase in pCR rate . -
pubmed
The methanolic extract ( ME ) was rich in
flavonoids , while the powdered leaves (
PL ) contained considerable amounts of macro- and microelements . -
pubmed
PL , ME and
GUA did not significantly alter the plasma levels of
triglycerides ,
high - density lipoproteins ,
very low - density lipoproteins ,
creatinine and
aspartate transaminase , and the
total protein levels in the kidney and liver tissues . -
pubmed
The
glyoxalase pathway evolved early in phylogeny to bring about rapid catabolism of
methylglyoxal , and an understanding of the role of
methylglyoxal and the
glyoxalases in many diseases is beginning to emerge . -
pubmed
Bonferroni
post - hoc testing showed there was statistically significant increases in metabolite ratios between 0700 and 1700 and these include different moieties of
N - acetylaspartate ,
creatine ,
choline ,
myo - inositol ,
lipids ,
fucose ,
glutathione , and
homocarnosine . -
pubmed
The production of reactive
oxygen species ( ROS ) as well as the response to oxidative stress and
ER stress were investigated . -
pubmed
Huntington ' s disease (
HD ) is a
monogenic neurodegenerative disorder caused by an expansion of the CAG trinucleotide repeat domain in the
huntingtin (
HTT ) gene , leading to an expanded poly -
glutamine (
polyQ ) stretch in the
HTT protein . -
pubmed
The results of
alizarin red staining and
alkaline phosphatase activity tests showed that
VD3 could not induce osteogenic differentiation in HUCMSCs . -
pubmed
IgG - conjugated AuNP were treated with
NaCN to dissolve the AuNP and restore the fluorescence emission that was quenched in the presence of the metallic colloids , followed by quantification of
fluorescein by spectrofluorimetry . -
pubmed
SKAP2 functions in reactive
oxygen species ( ROS ) production , phagocytosis , and
integrin - mediated migration by neutrophils . -
pubmed
Foliar
anthocyanins are hypothesised to function as photoprotective visible light screens , preventing over - excitation of the photosynthetic system , and decreasing the likelihood of photo - oxidative stress by absorbing green light and reducing the amount of light available to be absorbed by
chloroplasts in deeper tissue layers . -
pubmed
However , profiles of green light absorption by
chlorophyll within anthocyanic leaves were not shifted to reflect reduced absorption of green light by spongy mesophyll
chloroplasts . -
pubmed
Natural products can inhibit different coronavirus targets such as
S protein (
emodin ,
baicalin ) and viral enzymes replication such as
3CLpro (
Iguesterin ) ,
PLpro (
Cryptotanshinone ) ,
helicase (
Silvestrol ) , and
RdRp (
Sotetsuflavone ) . -
pubmed
Curcuminoids have been shown to exert their anti - GBM effects through regulation of angiogenesis , apoptosis , autophagy ,
metastasis , invasion as well as potential molecular targets , including
receptor tyrosine kinases ,
Sonic Hedgehog , and
NF - kappaB. -
pubmed
Pooled risk ratio ( RR ) of having any
sperm abnormality and
Testosterone ,
FSH , and
LH standardized mean differences among male cannabis users and non - users , and meta - regression analysis according to age , and year of publication . -
pubmed
Notably , we identified that
17 - DMAG , which targets the
ATP - binding site of
heat shock protein 90 (
Hsp90 ) , has a very high ( sub - micromolar range ) selective inhibition of the parasite growth . -
pubmed
It could be shown that the
5S RNA synthesized in vitro differed only very slightly in size from the mature species labeled in vivo and contained a few extra
nucleotides in some of the molecules . -
pubmed
Digestion of the in vitro product with
ribonuclease T1 and analysis of the
oligonucleotides on
DEAE - Sephadex A-25 in the presence of 7 M
urea revealed that the molar yield of the internal fragments agreed well with the expected theoretical values . -
pubmed
Neither
atropine nor
pramiverine were able to exert a tone depressing effect as pronounced as that of
SSB . -
pubmed
A significant correlation between the urinary
hEGF levels and
creatinine clearance ( r = 0.712 , p less than 0.001 ) and an inverse correlation between urinary
hEGF levels and fractional excretion of
sodium ( r = -0.406 , p less than 0.05 ) was demonstrated . -
pubmed
Pretreating the cells with
pertussis toxin to enhance
cAMP responses via inhibition of
Gi potentiated the effect of
bPTH -( 1 - 34 ) and
bPTH -( 2 - 34 ) and disclosed weak but detectable agonist action of
pbPTH -( 2 - 34 ) . -
pubmed
These results indicate that specific domains of the
PTH molecule are linked to activation of different second messenger pathways ; while the first two
amino acids are indispensable for activating the
cAMP system , generation of the [
Ca2+]i signal appears to involve a longer domain , including the
amino acid residue in position 3 . -
pubmed
[ Effects of
EGF and
retinoic acid on differentiation of
squamous cell carcinoma cells ] . -
pubmed
Modification of
gizzard myosin and reconstituted
actomyosin with
7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole at low and high ionic strength . -
pubmed
Treatment of reconstituted
gizzard actomyosin at 0.15 M or 0.6 M
KCl with the fluorescent
adenine analog
7 - chloro-4 - nitrobenzo-2 - oxa-1,3 - diazole ,
NBD - Cl , resulted in a significant decrease in the labeling of the
myosin from
actomyosin compared to that of
myosin alone . -
pubmed
The reagent was able to detect changes in the conformation of
myosin as the distribution of the label in the heavy and light chains of
myosin and
actin was different at 0.15 M and 0.6 M
KCl . -
pubmed
Jejunal mucosa is responsible for the absorption of
triglycerides and the production of lipoproteins [ chylomicrons ,
very - low - density lipoprotein (
VLDL ) ,
high - density lipoprotein (
HDL ) ] and
apolipoproteins (
B-48 ,
A - I , A - II ,
A - IV ,
C - II ) . -
pubmed
Statistically significant differences were found in the following parameters ( celiac versus control ): plasma levels of
triglycerides ( 70 versus 119 mg / dl ) ,
cholesterol content in
LDL ( 107 versus 67.7 mg / dl ) , protein content in
VLDL ( 6 versus 10 mg / dl ) , and level of
apoprotein A - I ( 112 versus 140 mg / dl ) . -
pubmed
No significant differences were found between the two groups in the serum levels of total
cholesterol , the
cholesterol content in
VLDL and
HDL , the protein content in
LDL and
HDL , and the level of
apoprotein B. -
pubmed
The complete
nucleotide sequence of
PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters . -
pubmed
On all five tobraviral RNA2
molecules sequenced to date , these sequences were found upstream of the
coat protein ORF in association with a strongly - conserved potential secondary structural element . -
pubmed
Upon stimulation with the antigen
ovalbumin (
OA ) of cells passively sensitized with
antiovalbumin (
anti - OA ) antibody , both cell types released
histamine . -
pubmed
Both lung mast cells and blood basophils released
histamine in response to the secretagogues
calcium ionophore
A23187 and
12 - O - tetradecanoylphorbol-13 - acetate (
TPA ) ;
TPA - induced
HR from mast cells was independent of added
calcium . -
pubmed
In basophils ,
TPA - induced
HR was only partially independent of added
calcium . -
pubmed
Phosphatidylserine had no effect on
HR provoked by antigen ,
Con - A , or
compound 48 / 80 . -
pubmed
For the latter
strains , the data suggested an important role of the
nucleotide at , and perhaps around , position 472 in determining a response to the addition of
ICF . -
pubmed
Amiloride selectively inhibits the
urokinase - type plasminogen activator . -
pubmed
The diuretic drug
amiloride , an inhibitor of
Na + uptake , competitively inhibits the catalytic activity of the
urokinase - type plasminogen activator (
u - PA ) , with a Ki of 7 X 10(-6 ) M. Generation of
plasmin , cleavage of peptide substrates , and interaction of
u - PA with a specific macromolecular
proteinase inhibitor are all prevented in the presence of the drug . -
pubmed
In contrast ,
amiloride does not affect the activity of either
tissue - type plasminogen activator ,
plasmin ,
plasma kallikrein or
thrombin . -
pubmed
The inhibition of
u - PA by
amiloride may be related to the previously reported inhibition of
u - PA - type enzymes by
Na + . -
pubmed
Amiloride or related compounds could prove useful in selectively controlling
u - PA - catalyzed extracellular proteolysis . -
pubmed
The microsomal
enzyme activity reduced free and bound
retinaldehyde to approximately the same extent , although the
aldehyde function of
retinaldehyde bound to
CRBP ( II ) was less accessible to chemical reducing agents than that of free
retinaldehyde . -
pubmed
Retinol bound to
CRBP ( II ) could not be oxidized by the microsomal activity in the presence of
NAD + , while free
retinol or
retinol bound to
bovine serum albumin was oxidized to
retinaldehyde . -
pubmed
A rise of serum
potassium ( mean + /- SD mmol / L ) from 4.1 + /- 0.3 to 4.4 + /- 0.4 ( p less than 0.05 ) in the placebo group and from 4.0 + /- 0.4 to 4.5 + /- 0.5 ( p less than 0.05 ) in the
timolol group was in multivariate analysis associated with
infarct size , estimated as cumulative
creatine kinase release , in the placebo group , and with the mean individual plasma
adrenaline concentrations in the
timolol group . -
pubmed
All the preparations were shown to contain a
ribonuclease which , in the presence of
ethylenediaminetetraacetic acid , led to ribosome breakdown as measured by the increase in acid - soluble
nucleotides . -
pubmed
In conjunction with a changed capacity for RNA synthesis it was observed that
abscisic acid - treated segments had a lowered soluble DNA - dependent
RNA polymerase level in comparison to
gibberellic acid - treated or illuminated segments . -
pubmed
Interaction of helper T cells and Lyb5 + B cells responding to
phosphorylcholine is
MHC - restricted . -
pubmed
[ Inhibition of
IgE antibody formation by tubercle bacilli and
muramyl peptide ] . -
pubmed
Seven major tryptic peptides of the
enzyme were found to be cross - linked to
tRNAPhe whereas six major
T1 - oligonucleotides were covalently linked to the protein , among these , the three cross - linked oligonucleotides previously described by Shoemaker and Schimmel ( J. Biol . -
pubmed
The occurrence of covalent cross - linking upon irradiation in the
tryptophan absorption band ( 302 nm ) strongly suggests the participation of this residue in the stabilization of the
tRNA enzyme complex . -
pubmed
Response of
insulin receptors to oral
glucose in normal subjects . -
pubmed
Effect of
celiprolol and
metoprolol on lipids ,
fibrinogen and airways function in
hyperlipidaemic hypertensives : a randomised double - blind long - term parallel group trial . -
pubmed
Significant
decreases in systolic and diastolic blood pressure and heart rate were observed compared with baseline (
DBP :
celiprolol -13.3 mm Hg , P & lt ; 0.0001 ;
metoprolol -14.3 mm Hg , P & lt ; 0.0001 ;
SBP :
celiprolol -18.2 mm Hg , P & lt ; 0.0001 ;
metoprolol -20.5 mm Hg , P & lt ; 0.0001 ; heart rate
celiprolol -4 beats / min , P & lt ; 0.003 ;
metoprolol -12 beats / min , P & lt ; 0.0001 ) . -
pubmed
When compared with baseline values
celiprolol significantly reduced serum
low density lipoprotein cholesterol (
LDL - C ) ( -5.8 % , P = 0.0401 ) and produced a slight increase in
high density lipoprotein cholesterol (
HDL - C ) which approached statistical significance ( 4.1 % , P = 0.0659 ) . -
pubmed
Metoprolol significantly increased serum
triglycerides ( 32 % , P = 0.0001 ) and the total /
HDL - C ratio ( 7.4 % , P = 0.0192 ) . -
pubmed
Compared with
metoprolol ,
celiprolol significantly reduced
LDL - C ( -7.3 % , P = 0.0062 ) , total
cholesterol ( -4.5 % , P = 0.0085 ) ,
apoliproprotein B ( -10.1 % , P = 0.0001 ) , the
apolipoprotein B / A1 ratio ( -10.9 % , P = 0.0001 ) , the total
cholesterol /
HDL - C ratio ( -10.8 % , P = 0.0001 ) and
triglycerides ( -24.8 % , P = 0.0001 ) , and significantly increased
HDL - C ( 6.0 % , P = 0.0043). -
pubmed
Serotonin antagonism did not noticeably affect the
LH pulse attributes ( frequencies , interpulse intervals , amplitudes ) . -
pubmed
Gonadotrophin and
PRL release in response to
GnRH administration was unaltered by
ondansetron treatment . -
pubmed
The identical effects of
glycine with that of
glutamate in certain concentrations is consistent with previous reports that
glycine and
N - methyl - D - aspartate (
NMDA ) act as coagonists of a common
excitatory amino acid receptor . -
pubmed
Effect of chronic central treatment with the
acetylcholine analog
methylcarbamylcholine on
cortical nicotinic receptors : correlation between receptor changes and behavioral function . -
pubmed
Chronic administration of
nicotine results in increased numbers of specific
nicotinic acetylcholine receptors (
nAChRs ) within the central nervous system . -
pubmed
Chronic administration of
MCC reversibly increased the apparent Bmax and Kd of cortical
nAChRs in a dose- and time - dependent manner . -
pubmed
Like chronic
MCC treatment , chronic
DHBE increased the number of
[ 3H ] cytisine binding sites but without concomitant development of behavioral tolerance. -
pubmed
In 0.1 M
KCl between pH
6.3 and pH 7.3 , filtered samples were turbid , suggesting the presence of high molecular weight aggregates ( mesophase ) , which could be removed by ultrafiltration . -
pubmed
METHODS : Blood pressure , demographic factors ,
plasma renin activity ( PRA ) , urinary
electrolyte excretion and bioassayable
PHF - like activity were determined in the fasted state on basal and altered dietary salt intakes . -
pubmed
This latter finding provides a strong warning that
p53 immunochemistry with available
reagents can not be used to determine which
tumors have mutations of
p53 . -
pubmed
Nitric oxide (
NO ) , a
free radical gas that acts as a neural messenger , is produced from
L - arginine by the enzyme
nitric oxide synthase (
NOS ) . -
pubmed
[ 3H ] L - NOARG binding was enriched in discrete neuronal populations and the distribution observed was identical to that previously described for
NOS using immunohistochemistry , in situ hybridization and
NADPH - diaphorase staining . -
pubmed
[ 3H ] L - NOARG autoradiography should be a useful method for the further characterization and quantification of
NOS in brain and other tissues . -
pubmed
Bradykinin analogs were synthesized by the solid phase method , using Boc strategy on
PAM or Merrifield resins . -
pubmed
The same analog completely inhibits
bradykinin - evoked
cytokine expression by mononuclear cells . -
pubmed
Pulse oximetry has become the leading technique for monitoring
hemoglobin oxygen saturation whenever a risk of
hypoxia exists . -
pubmed
Since it uses 2 wavelengths , it can only measure
oxyhemoglobin and reduced
hemoglobin . -
pubmed
Ethanol feeding impairs innate immunity and alters the expression of Th1- and Th2 - phenotype
cytokines in murine Klebsiella
pneumonia . -
pubmed
The prolonged and excessive consumption of
alcohol has been shown to predispose the host to a variety of
infectious complications , which may be due , in part , to the inability to produce important activating
cytokines . -
pubmed
In summary , our studies indicate that
ethanol ingestion results in a profound suppression of lung bacterial clearance and decreased survival in Klebsiella
pneumonia , which occurs in association with a shift in the balance of lung cytokine mRNA and protein expression favoring Th2- rather than Th1 - phenotype
cytokines . -
pubmed
Understanding transcription initiation of the
endothelial nitric - oxide synthase (
eNOS ) gene appears pivotal to gaining a comprehensive view of
NO biology in the blood vessel wall . -
pubmed
EPR of a VO2 + -doped
Mg ( ND4 ) 2 ( SO4 ) 2 -
pubmed
Of the 10 models generated by the program Catalyst /
Hypo , hypotheses including
hydrogen - bond acceptor (
HBA ) , hydrophobic ( Hp ) , and hydrophobic aliphatic (
HpAl ) features were considered important and predictive in evaluating OA agonists . -
pubmed
An
HBA and four hydrophobic features are the minimum components of an effective OA agonist - binding hypothesis , which resembles the results of binding activity to
locust OAR3 . -
pubmed
Active agonists mapped well onto all of the features of the hypothesis , such as
HBA , Hp , and
HpAl features . -
pubmed
Enoxaparin was administered 2 h
post -
TBI at 0.5 mg / kg i.v . -
pubmed
The
neurological deficit evaluated with a 9 - point scale was also improved with
enoxaparin at 0.5 + 4 x 1 mg / kg 1 week
post -
TBI ( p & lt ; 0.05 ) . -
pubmed
The
cognitive impairment evaluated with a Lashley maze task was improved with
enoxaparin ( 0.5 + 4 x 1 mg / kg ) from 48 h ( p & lt ; 0.05 ) to 2 weeks
post -
TBI ( p & lt ; 0.01 ) . -
pubmed
Aza analogues of
thalidomide : synthesis and evaluation as inhibitors of
tumor necrosis factor - alpha production in vitro . -
pubmed
These
5 - phthalimidopyrimidines as well as
phthalimido-2,4 - difluorobenzenes were designed as analogues of
thalidomide , a well known inhibitor of
TNF - alpha production . -
pubmed
HT ,
DM , low levels of
high - density lipoprotein cholesterol (
HDL - C ) and
hypertriglyceridemia remained as significant
coronary artery disease (
CAD ) risk factors even after controlling for age , sex , hospital , smoking ,
alcohol use ,
body mass index and leisure time physical activity . -
pubmed
On multivariate analysis , preischemic FAC(AUC ) was mainly affected by sex , peak FAC(AUC ) was affected by sex and
systolic BP , percent changes were affected by plasma high - density and
low - density lipoprotein cholesterol , peak time was affected by age and
body mass index , and descent time was affected by plasma
triglyceride levels . -
pubmed
The enzyme
L - phenylalanine ammonia - lyase (
PAL ) catalyzes the non - oxidative deamination of
L - phenylalanine to form
trans - cinnamic acid and
ammonia . -
pubmed
X - ray diffraction ( XRD ) , Brunauer Emmett Teller method ( BET ) , electron probe X - ray micro analysis ( EPMA ) and temperature programmed reduction (
TPR ) by
H2 were used to characterize a series of supported
copper catalysts . -
pubmed
Inflammation induces an up - regulation of neuropeptide
tyrosine (
NPY ) and its receptors in the dorsal horn , suggesting an important role in nociceptive transmission . -
pubmed
The ipsilateral effect of
NPY was completely inhibited with the potent
Y1 antagonist ,
BIBO 3304 ( 3 microg ) , but not the
Y2 antagonist ,
BIIE 0246 . -
pubmed
When administered alone ,
BIBO 3304 ( but not
BIIE 0246 ) slightly decreased thermal PWL on the side ipsilateral ( 25 % change ) , but not contralateral , to CFA injection ; this suggests that
inflammation strengthens inhibitory
NPY tone . -
pubmed
Sleep attacks in
Parkinson ' s disease induced by
Entacapone , a
COMT - inhibitor . -
pubmed
Entacapone , a
catechol - O - methyl transferase inhibitor , alters the pharmacokinetics of
levodopa , leading to increase of
levodopa concentration in plasma and brain . -
pubmed
They were submitted to clinical history , laboratory studies ( blood count cell ,
serum IgE levels , eosinophils of nasal mucosa ) , roentgenograms ( paranasal
sinus and esophagus - gastroduodenal series ) and
allergy skin tests with 32 allergens . -
pubmed
Testosterone significantly increased
claudin-11 expression in cultured Sertoli cells . -
pubmed
Angiotensin II blocks
nicotine - mediated neuroprotection against
beta - amyloid ( 1 - 42 ) via activation of the
tyrosine phosphatase SHP-1 . -
pubmed
We also showed that preincubation with
angiotensin II (
Ang II ) , functioning via the
angiotensin II type 2 ( AT2 ) receptor , blocked both the
nicotine - induced activation of
JAK2 and its neuroprotection against
Abeta ( 1 - 42 ) . -
pubmed
Therefore , the potential biological significance of
AT2 receptor - induced effects on both the
nicotine - induced activation of
JAK2 and its neuroprotection against
Abeta ( 1 - 42 ) led us to investigate whether
SHP-1 activation could be involved in this process . -
pubmed
We found that
Ang II induced the activation of
SHP-1 and that an antisense against
SHP-1 not only augmented the
nicotine - induced
tyrosine phosphorylation of
JAK2 but also blocked the
Ang II neutralization of the
nicotine - induced neuroprotection . -
pubmed
The adsorption of 3
barbiturates --
phenobarbital ,
mephobarbital , and
primidone -- from simulated intestinal fluid ( SIF ) , without
pancreatin , by activated
carbon was studied using the rotating bottle method . -
pubmed
rAd MEK1EE
infection induced morphological changes and DNA synthesis which were inhibited by the
MEK1,2 inhibitor
PD184352 . -
pubmed
This secretion is inhibited by
PD184352 , but not by
ErbB inhibitors . -
pubmed
As UV - B had no influence on
CYP1A content in larval pike ,
retene ( 9 - 82 microg / l ) induced this protein substantially with and without UV - B. -
pubmed
Neither UV nor
retene changed
SOD activity significantly . -
pubmed
Changing the extracellular matrix protein to
laminin - rich Matrigel , the monolayers re - formed islet - like clusters that secreted
insulin in a
glucose - responsive fashion . -
pubmed
Myotoxic
bupivacaine was injected into SOL and
EDL and the muscles were either denervated or neuromuscularly blocked by
tetrodotoxin (
TTX ) on the sciatic nerve . -
pubmed
Three to 10 or 30 days later , denervated SOL or
EDL , or innervated but neuromuscularly blocked
EDL received a slow 20 Hz stimulus pattern through electrodes implanted on the muscles or along the fibular nerve to
EDL below the
TTX block . -
pubmed
Stimulated denervated and stimulated innervated (
TTX blocked )
EDL expressed the same amounts of type 1 ,
2A , 2X and
2B MyHC proteins . -
pubmed
ADC and NADC TUNEL positive dying cells in resin sections observed by light microscopy were examined again by transmission
electron microscopy using a re - embedding procedure . -
pubmed
High - affinity capture of
proteins by
diamond nanoparticles for mass spectrometric analysis . -
pubmed
Sodium nitroprusside led to an increase in
CBF and CBV ( +30 % and +37 % , respectively , P<0.05 ) , whereas no change in CMRO2 was noted . -
pubmed
Linear regression analysis of
CBF values as a function of MABP confirmed that
CBF increases when MABP is reduced by
SNP . -
pubmed
Carbonyl content in
RPE cells increased with increasing concentrations of oxidants or increasing duration of exposure with high reproducibility , validating ELISA for
carbonyl content as a valuable quantitative measure of oxidative damage . -
pubmed
Compared to other cell types ,
RPE cells were able to survive exposure to
H2O2 quite well and exposure to
paraquat extremely well . -
pubmed
This study suggests that multiple types of oxidant stress should be used to probe the vulnerabilities of the retina and
RPE in vivo , and that ELISA for
carbonyl content provides a valuable tool for quantitative assessment of oxidative damage for such studies . -
pubmed
Despite limited data ,
polymyxin B (
PB ) is increasingly used clinically as the last therapeutic option for multidrug - resistant ( MDR ) gram - negative
bacterial infections . -
pubmed
Mixture and concentration - response experiments demonstrated a suppression of phagocytosis by non - coplanar
PCBs suggesting a previously unrecognized
aryl hydrocarbon receptor (
AhR)-independent pathway . -
pubmed
Our results are cause for concern as they suggest an
AhR - independent pathway through which non - coplanar
PCBs modulate phagocytosis , the immune system 's first line of defense , possibly increasing the risk to developing
infectious disease . -
pubmed
Treatment with 0.22 mg of
MGA / head / d for 14 d had a detrimental effect on fertility when insemination was carried out during the first estrus
post treatment . -
pubmed
A reciprocal substitution of
amino - acid residues with a
hydroxyl group at these positions , T141S or S150 T , produced a broadened cation selectivity of the
antiporter for K+ , in addition to
Na + and
Li+ . -
pubmed
Site - directed mutagenesis of
Ser150 showed that while the replacement of
Ser150 with a small hydrophobic (
valine ) or negatively charged (
aspartate )
amino acid did not produce a significant change in ZrSod2 - 22p substrate specificity , the introduction of a positive charge at this position stopped the activity of the
antiporter . -
pubmed
Mean seasonally adjusted
25 ( OH ) D concentration was 71.1 nmol / L. Serum
25 ( OH ) D also exhibited the expected inverse curvilinear relationship with
serum parathyroid hormone (
PTH ) , with the inflection point of the curve located at approximately 80 nmol / L. Supplements containing
vitamin D were regularly taken by 59 % of the cohort ( median dose : 200 IU / d ) . -
pubmed
Natural peptide agonists of
corticotrophin - releasing factor ( CRF ) receptors bind to the receptor by a two - site mechanism as follows : the
carboxyl end of the ligand binds the N - terminal extracellular domain (
ECD ) of the receptor and the
amino portion of the ligand binds the extracellular
face of the seven transmembrane region . -
pubmed
Two hydrophobic residues (
Met(38 ) and
Ile(41 ) ) as well as two
amide groups (
Asn(34 ) and the C - terminal
amide ) on one
face of the helix defined the binding epitope of the antagonist . -
pubmed
Serum
zinc levels of diseased calves were lower than healthy ones and this difference were found to be important statistically ( p<0.001 ) , whereas there is no statistical difference on the levels of lymphocyte , monocyte , granulocyte , erythrocyte ,
hemoglobin , hematocrit , and mean corpuscular volume between groups . -
pubmed
Transcription factors , including
PPAR coactivator ( PGC )- 1beta ,
PGC-1alpha ,
estrogen - related receptor alpha , and
PPARalpha , were suppressed in both models and induced by
RSG . -
pubmed
The effects of
RSG on adipose mitochondrial genes were confirmed by quantitative RT - PCR and further supported by mitochondrial staining , mitochondrial DNA quantification , and
citrate synthase activity . -
pubmed
Published data on the concentration dependence of osmotic pressure of solutions of
bovine serum albumin in 0.15 M
NaCl at concentrations up to greater than 400 g / L are shown to be described to within experimental uncertainty by a simple one - parameter model in which protein molecules are represented by effective hard spherical particles . -
pubmed
We show that
emerin interacts with
tubulin , and that
nocadozole - treated wild - type cells phenocopy the detached centrosome characteristic of
emerin - null / deficient cells . -
pubmed
Phorbol 12 - myristate 13 - acetate - activated
protein kinase C increased migratory activity of subconjunctival fibroblasts via
stress - activated protein kinase pathways . -
pubmed
The fibroblasts were treated with
phorbol 12 - myristate 13 - acetate (
PMA ) to activate
PKC . -
pubmed
PMA treatment in the subconjunctival fibroblasts elicited phosphorylation of
SAPKs , including
c - jun N - terminal kinase (
JNK ) and
p38 mitogen - activated protein (
MAPK ) . -
pubmed
Furthermore , specific inhibitors of
JNK and
p38MAPK blocked
PMA - induced migration of the subconjunctival fibroblasts . -
pubmed
The phosphorylation of
c - jun and
heat shock protein 27 , downstream effectors of
JNK and
p38MAPK , respectively , was upregulated by
PMA treatment . -
pubmed
CONCLUSIONS :
PMA - activated
PKC increased migratory activity , and
SAPK pathways were critical for migration of subconjunctival fibroblasts . -
pubmed
We assayed fasting total ,
high - density lipoprotein (
HDL ) , and
low - density lipoprotein (
LDL )
cholesterol ;
LDL /
HDL cholesterol ratio ;
triacylglycerols (
TG ) ; and
C - reactive protein (
CRP ) . -
pubmed
Dietary GI was significantly associated with
HDL and
LDL cholesterol ,
LDL /
HDL cholesterol ratio , TG , and
CRP ( comparing top to bottom quintile difference in
HDL cholesterol = -2.6 mg / dL ,
LDL cholesterol = 2.2 mg / dL ,
LDL /
HDL cholesterol ratio = 0.16 ,
TG = 12 mg / dL , and
CRP = 0.21 mg / L ) . -
pubmed
Dietary
GL was associated with
HDL cholesterol ,
LDL /
HDL cholesterol ratio , and
TG ( comparing top to bottom quintile
HDL cholesterol = -4.9 mg / dL ,
LDL /
HDL cholesterol ratio = 0.24 , and
TG = 13 mg / dL ) . -
pubmed
Upon stimulation by growth factors and chemokine ligands ,
integrin - linked kinase mediates the phosphorylation of
Akt Ser473 , and
glycogen synthase kinase-3 . -
pubmed
A ternary complex of
hydroxycinnamoyl - CoA hydratase - lyase (
HCHL ) with
acetyl - CoA and
vanillin gives insights into substrate specificity and mechanism . -
pubmed
HCHL (
hydroxycinnamoyl - CoA hydratase - lyase ) catalyses the biotransformation of
feruloyl - CoA to
acetyl - CoA and the important flavour - fragrance compound
vanillin (
4 - hydroxy-3 - methoxybenzaldehyde ) and is exploited in whole - cell systems for the bioconversion of
ferulic acid into natural equivalent
vanillin . -
pubmed
The reaction catalysed by
HCHL has been thought to proceed by a two - step process involving first the hydration of the double bond of
feruloyl - CoA and then the cleavage of the resultant
beta - hydroxy thioester by retro - aldol reaction to yield the products . -
pubmed
Kinetic analysis of active - site residues identified using the crystal structure of
HCHL revealed that while
Glu-143 was essential for activity ,
Ser-123 played no major role in catalysis . -
pubmed
Structures of WT ( wild - type )
HCHL and of the S123A mutant , each of which had been co - crystallized with
feruloyl - CoA , reveal a subtle helix movement upon ligand binding , the consequence of which is to bring the
phenolic hydroxyl of
Tyr-239 into close proximity to
Tyr-75 from a neighbouring subunit in order to bind the
phenolic hydroxyl of the product
vanillin , for which electron density was observed . -
pubmed
The active - site residues of ligand - bound
HCHL display a remarkable three - dimensional overlap with those of a structurally unrelated
enzyme ,
vanillyl alcohol oxidase , that also recognizes p - hydroxylated aromatic substrates related to
vanillin . -
pubmed
The data both explain the observed substrate specificity of
HCHL for
p - hydroxylated cinnamate derivatives and illustrate a remarkable convergence of the molecular determinants of ligand recognition between the two otherwise unrelated
enzymes . -
pubmed
Fasting blood
glucose ,
HDL ,
LDL and
TSH levels , age ,
glucose intolerance low serum
folic acid and significantly increased
polyneuropathy risk . -
pubmed
Age , weight ,
body mass index , high fasting , 30 , 60- , 90- , 120 - min serum
glucose ,
insulin and
HgA1c levels were risk factors for
glucose intolerance . -
pubmed
Potassium channel interacting proteins (
KChIPs ) are members of a family of
calcium binding proteins that interact with Kv4
potassium ( K(+ ) ) channel primary subunits and also act as transcription factors . -
pubmed
To address this question , we studied the association of
AnxA6 with model phospholipid membranes by combining the techniques of
quartz crystal microbalance with dissipation monitoring ( QCM - D ) , ( cryo- ) transmission electron microscopy (
TEM ) and atomic force microscopy (
AFM ) . -
pubmed
We show that
AnxA6 presents two modes of association with lipid membranes depending on
Ca ( 2 +)-concentration . -
pubmed
At low
Ca ( 2 +)-concentration ( approximately 60 - 150microM ) ,
AnxA6 binds to membranes via its two coplanar annexin modules and is not able to associate two separate membranes . -
pubmed
At high
Ca ( 2 +)-concentration ( approximately 2mM ) ,
AnxA6 molecules are able to bind two adjacent phospholipid membranes and present a conformation similar to the
AnxA6 3D crystallographic structure . -
pubmed
Primary cutaneous
CD30 +
anaplastic large - cell lymphoma with generalized
skin involvement and involvement of one peripheral lymph node , successfully treated with low - dose oral
etoposide . -
pubmed
Lactate and attentional measurements were performed before ( Block 1 ,
B1 ) and after the fatiguing exercise ( B2 ) and at the end of a recovery period (
B3 ) . -
pubmed
In this study , archival
formalin - fixed , paraffin - embedded
breast cancer specimens at various developmental stages were prepared as tissue microarrays ( TMAs ) and examined for the expressions of
piwil2 ,
estrogen receptor (
ER ) ,
progesterone receptor (
PR ) and a cell proliferation marker
Ki67 by immunohistochemical ( IHC ) staining and
human epidermal growth factor receptor 2 (
HER2 ) by fluorescence in situ hybridization ( FISH ) . -
pubmed
Three
opioid receptors (
mu , micro ; delta , delta ; and
kappa ,
kappa ) that mediate opioid effects have been identified by molecular cloning : delta (
enkephalin - preferring ) , kappa (
dynorphin - preferring ) , and micro (
morphine and ss - endorphin - preferring ) ( 1 ) . -
pubmed
Carfentanil (
CFN ) is a highly potent and selective
micro opioid receptor agonist with subnanomolar affinity ( Kd , 0.08 nM ) . -
pubmed
[ ( 11 ) C ] CFN is being developed as a positron emission tomography ( PET ) agent for the non - invasive studies of
micro opioid receptors in the brain . -
pubmed
On the other hand ,
N1 '- methylnaltrindole (
MeNTI ) is a potent and selective
delta opioid receptor antagonist ( Ki , 0.02 nM ) . -
pubmed
[ ( 11 ) C ] MeNTI has been prepared for studying the role of
delta opioid receptor in health and disease by PET imaging . -
pubmed
[ The influence of live ammunition manoeuvres on the levels of serum hormones (
cortisol ,
testosterone , gastrin , and
insulin ) ] . -
pubmed
HDACi also decreased the expression of
SGLT1 , an active
glucose transporter found to be stabilized by
EGFR , and suppressed the
glucose uptake of
cancer cells . -
pubmed
This article describes the adaptation of a simple colorimetric assay for inorganic
pyrophosphate to the enzyme
3 - deoxy - d - manno - octulosonate cytidylyltransferase (
CMP - KDO synthetase ,
KdsB ,
EC 2.7.7.38 ) , a key enzyme in the biosynthesis of lipopolysaccharide ( LPS ) in Gram - negative organisms . -
pubmed
This assay is particularly useful because it can be combined with the
malachite green ( MG ) assay for inorganic
phosphate to form an assay system capable of determining inorganic
phosphate and inorganic
pyrophosphate in the same solution ( the MG /
EK ( eikonogen reagent ) assay ) . -
pubmed
We tested this potential using two enzymes ,
KdsB and
KdsC , involved in the biosynthesis and use of the key bacterial
8 - carbon sugar ,
KDO . -
pubmed
They hydrolysed
aesculin , starch and
urea , but not
casein or DNA . -
pubmed
The dorsal medulla was exposed , and
phenylephrine ( 2 mM , 10 nl ) and then the
alpha ( 1 )- adrenoceptor antagonist
prazosin ( 0.2 mM , 3 x 40 nl ) were microinjected into the XII nucleus while XII nerve activity ( XIIa ) was recorded . -
pubmed
poly ( SMA - co - PEGMEMA - co - EDMA ) with 15 % ( w / w )
PEGMEMA ) demonstrated nearly 35 % reduction in retention of
polycyclic aromatic compounds and greater than 40 % increase in retention of
phenols compared to a hydrophobic
C18 monolithic column . -
pubmed
Again , incorporation of 15 % ( w / w )
PEGMEMA in the monolith increased the extraction efficiency of
phenols in water from approximately 20 % to 67 - 92 % compared to a hydrophobic
C18 monolithic material . -
pubmed
The
OTC loading rates at between 22.22 and 133.33 g
OTC m(-3 ) d(-1 ) improved the hydrolysis of molasses - COD ( k ) , the maximum specific utilization of molasses - COD ( k(mh ) ) and the maximum specific utilization rate of
TVFA ( k(
TVFA ) ) . -
pubmed
Therefore , we examined whether
TFDG and
EGCG affect
MMP activity and osteoclast formation and differentiation in vitro . -
pubmed
Numbers of multinucleated osteoclasts and
actin rings decreased in
polyphenol - treated cultures relative to control cultures . -
pubmed
MMP-9 mRNA levels declined significantly in
TFDG - treated osteoclasts in comparison to control osteoclasts . -
pubmed
TFDG and
EGCG inhibited the formation and differentiation of osteoclasts via inhibition of
MMPs . -
pubmed
TFDG may suppress
actin ring formation more effectively than
EGCG . -
pubmed
In silico analyses and genetic complementation tests showed that
entA locates next to an
entB ortholog , which codes for a putative protein that contains the
isochorismatase lyase domain , which is needed for
DHBA biosynthesis from
isochorismic acid , but lacks the
aryl carrier protein domain , which is needed for tethering activated
DHBA and completion of
siderophore biosynthesis . -
pubmed
Spermatocytes lacking
zw10 or
rint1 function assemble regular central spindles and
acto -
myosin rings , but furrow ingression halts prematurely due to defective plasma membrane addition . -
pubmed
In order to conduct the mentioned evaluation the restriction fragment length polymorphisms (
ApaI ,
BsmI and
TaqI ) in the
Vitamin D receptor gene were researched in three groups of patients : patients suffering only from
psoriasis vulgaris , patients suffering only from
diabetes mellitus , and patients suffering at the same time from both diseases . -
pubmed
The
ATP2C1 gene encodes for the secretory pathway
calcium (
Ca2 +)-ATPase pump (
SPCA1 ) , which localizes along the secretory pathway , mainly in the trans - Golgi . -
pubmed
If dysregulated , the composition of the
SPCA1 isoform pool could diverge from the physiological status , affecting cytosolic
Ca2 + -signaling , and in turn perturbing cell division , leading to cell
death or to neoplastic transformation . -
pubmed
The reaction of
( NBu4 )[ ReOCl4 ] with
H2L1 and two equivalents of
ammonium thiocyanate , however , gives in a one -
pot reaction [
ReO ( NCS ) 2 ( HL1 ) ] ( 8) , in which the pro - ligand
H2L1 is only singly deprotonated . -
pubmed
These results suggest that highly malignant differentiated
thyroid cancer can be detected by monitoring
HER2 status and telomere shortening , and that
trastuzumab therapy may be effective for
refractory thyroid cancer . -
pubmed
H2 S had little effect on
eNOS protein expression in ECs . -
pubmed
L - cysteine , a precursor of
H2 S , stimulated
NO production whereas blockage of the activity of
H2 S - generating enzyme ,
cystathionine gamma - lyase (
CSE ) , inhibited this action . -
pubmed
LY294002 (
Akt /
PI3 - K inhibitor ) or
SB203580 (
p38 MAPK inhibitor ) abolished the effects of
H2 S on
eNOS phosphorylation ,
NO production , cell proliferation and tube formation . -
pubmed
Blockade of
NO production by
eNOS - specific siRNA or
nitro - L - arginine methyl ester (
L - NAME ) reversed , but
eNOS overexpression potentiated , the proliferative effect of
H2 S on ECs . -
pubmed
Our results suggest that
H2 S stimulates the phosphorylation of
eNOS through a
p38 MAPK and
Akt - dependent pathway , thus increasing
NO production in ECs and vascular tissues and contributing to
H2 S - induced angiogenesis . -
pubmed
Here , we demonstrate that
Pds5 proteins are essential for cohesion establishment by allowing
Smc3 acetylation by the cohesin
acetyl transferases (
CoATs )
Esco1/2 and binding of
Sororin . -
pubmed
NaHS pretreatment restored both the
DDAH activity and intracellular
l - arginine levels . -
pubmed
Extra- and intra - cellular partitions of
ADMA seem to depend on transport proteins as well as the
DDAH activity . -
pubmed
METHODS : The effects of
PGD2 ,
IL-25 , and
IL-33 on the cell migration ,
cytokine production , gene regulation , and receptor expression of ILC2s were measured with chemotaxis , ELISA , Luminex , flow cytometry , quantitative RT - PCR , and QuantiGene assays . -
pubmed
RESULTS :
PGD2 binding to
CRTH2 induced ILC2 migration and production of
type 2 cytokines and many other
cytokines . -
pubmed
CONCLUSIONS :
PGD2 is an important and potent activator of ILC2s through
CRTH2 mediating strong proallergic inflammatory responses . -
pubmed
Thus , the photocatalytic degradation of
4 - chlorophenol ( 4 -
CP ) , a potent endocrine disrupting chemical in aqueous medium was investigated by both pure and
Mg - doped
ZnO NPs under UV - light irradiation in the present study . -
pubmed
The influence of the
Mg content on the structure , morphology ,
PL character and photocatalytic activity of
ZnO NPs were investigated systematically . -
pubmed
The search terms were '
gout ' , '
gouty arthritis ' , '
hyperuricemia ' , '
uric acid ' , '
xanthine oxidase (
XO ) inhibitor ' , ' uricosuric ' , '
urate transporter 1(
URAT1 ) ' and '
glucose transporter 9 (
GLUT9 ) ' . -
pubmed
In in vitro studies ,
flavonoids , alkaloids , essential oils ,
phenolic compounds ,
tannins ,
iridoid glucosides , and
coumarins show the potential of anti -
gout effects by their
XO inhibitory action , while
lignans ,
triterpenoids and
xanthophyll are acting through their anti - inflammatory effects . -
pubmed
Adrenomedullin (
AM ) belongs to a
calcitonin gene - related peptide ( CGRP ) family and has been demonstrated to recruit
CGRP following chronic use of
morphine and
neuronal nitric oxide synthase (
nNOS ) in
inflammation . -
pubmed
The present study investigated the possibility that
AM initiates the changes of other molecules contributing to the development of
morphine tolerance in its chronic use . -
pubmed
co - administration of the
AM receptor antagonist
AM22 - 52 ( 35.8 mug ) inhibited tolerance to
morphine - induced analgesia while a daily injection of the
AM receptor agonist
AM1 - 50 ( 8 mug , i.t . -
pubmed
Persistent exposure of cultured dorsal root
ganglion ( DRG ) explants to
morphine ( 3.3 muM ) for 4 days resulted in an increase in
AM and
CGRP mRNA levels . -
pubmed
Particularly , the co - administration of
AM22 - 52 ( 35.8 mug ) inhibited the
morphine - induced alterations in
nNOS and
BAM22 . -
pubmed
These results indicated that the increase in
nNOS and
CGRP expressions and the decrease in
BAM22 were attributed to the increased
AM receptor signaling induced by chronic
morphine . -
pubmed
The present study supports the hypothesis that the enhancement of
AM bioactivity triggered upregulation of pronociceptive mediators and downregulation of
pain - inhibiting molecule in a cascade contributing to the development of
morphine tolerance . -
pubmed
Concentrations of
prostaglandin E2 (
PGE2 ) ,
transforming growth factor beta (
TGF - beta ) and
interleukin-10 (
IL-10 ) were measured using enzyme - linked immunosorbent assay ( ELISA ) kits . -
pubmed
difficile carriage and chemotherapy regimen , antibiotic drug use , or immunosuppressive mediators , such as
prostaglandin E2 (
PGE2 ) ,
transforming growth factor beta (
TGF - beta ) , or
interleukin-10 (
IL-10 ) . -
pubmed
Septin6 and
Septin7 GTP binding proteins regulate
AP-3- and
ESCRT - dependent multivesicular body biogenesis . -
pubmed
Septins (
SEPTs ) form a family of
GTP - binding proteins implicated in cytoskeleton and membrane organization , cell division and host / pathogen interactions . -
pubmed
Characterization of the mutants showed that
PAT2 and
ACK1 activity in
chloroplasts plays a dominant role ( relative to
ACK2 and
PAT1 in mitochondria ) in producing
acetate under dark , anoxic conditions and , surprisingly , also suggested that Chlamydomonas has other pathways that generate
acetate in the absence of
ACK activity . -
pubmed
The
strains were cultured on chocolate agar plates in a 5 %
CO2 atmosphere at 37 C and identified by colony morphology , Gram staining and
oxidase reaction , followed by
carbohydrate utilization test . -
pubmed
Anti - nociceptive effects of
GL were assessed by using
acetic acid - induced writhing , hot plate test and
formalin test , as well as evaluation of spontaneous locomotor activity and motor performance . -
pubmed
The results showed that
GL significantly reduced
xylene - induced
ear edema , carrageenan - induced paw
edema , and
acetic acid - induced vascular permeation . -
pubmed
Additionally ,
GL significantly inhibited the
nociceptions induced by
acetic acid and
formalin . -
pubmed
The
CCKA - receptor requires the
tyrosyl residue in the C - terminal bioactive site of
CCK to be O - sulfated , whereas , the
CCKB - receptor binds irrespective of sulfation . -
pubmed
BACKGROUND : Dietary factors such as high -
sodium or high - fat (
HF ) diets have been shown to induce a proinflammatory phenotype . -
pubmed
[ ( 18 ) F ]- FET PET was quantitated by calculation of maximum and mean standardized uptake values within a universal volume - of - interest ( VOI ) corrected for healthy background ( SUVmax /
BG , SUVmean /
BG ) . -
pubmed
Linezolid use was a risk for mortality in patients with APACHE
II scores of & gt;=14 . -
pubmed
Reduced actions of
NO and
brain hypoperfusion trigger increased production of
amyloid - beta that inhibits endothelial function , thus establishing a vicious cycle for impairing brain circulation . -
pubmed
In contrast , the Y146S / Y154S dimerization - defective mutant displays a severe
dNTPase defect in vitro , but is indistinguishable from WT in its ability to deplete cellular
dNTP pools and to restrict HIV replication . -
pubmed
The effect of
H2O2 initial concentration and dosage on
IMZ degradation rate , average oxidation state (
AOS ) , organic acid concentration , oxidation , and mineralization percentage after photo - Fenton process was determined . -
pubmed
The effect of the oxidation degree , limited by
H2O2 concentration and dosage , on the percentage of mineralization obtained after the biological treatment was determined and explained in terms of changes in
AOS . -
pubmed
We have paired the
gfap promoter region with the
glutamate reporter molecule ,
iGluSnFR , to drive expression in glial cells throughout the nervous system . -
pubmed
Tg(
gfap :
iGluSnFR ) is expressed on the glial membrane of Muller glia cells in the retina , which rapidly respond to stimulation and the release of extracellular
glutamate . -
pubmed
As glial cells are associated with most , if not all , synapses , Tg(
gfap : iGluSnFR ) is a novel and exciting tool to measure neuronal activity and extracellular
glutamate dynamics in many regions of the nervous system . -
pubmed
Cryogenian cap
carbonates that overlie Sturtian
glacial deposits were formed during a
post -
glacial transgression . -
pubmed
Here , we describe microfossils from the Kakontwe Formation of Zambia and the Taishir Formation of Mongolia - both Cryogenian age ,
post - Sturtian cap
carbonates - and investigate processes involved in their formation and preservation . -
pubmed
Effect of the
GSTM1 genotype on the biomarkers of exposure to
polycyclic aromatic hydrocarbons : Meta - analysis . -
pubmed
The role of
glutathione S - transferase Mu 1 (
GSTM1 ) in the biomonitoring of
polycyclic aromatic hydrocarbons (
PAHs ) is not clear . -
pubmed
Our purpose has been to evaluate the influence of
GSTM1 genotypes on
1 - hydroxypyrene (
1 - OHP ) , deoxyribonucleic acid ( DNA ) adducts , and micronucleus frequency in both occupational and non - occupational populations of null and active
GSTM1 carriers . -
pubmed
In the non - occupationally exposed general population ,
1 - OHP and micronucleus frequency were significantly higher in the null
GSTM1 carriers . -
pubmed
In conclusion ,
GSTM1 genotypes may affect the urinary
1 - OHP in the non - occupationally exposed general population , and micronucleus frequency in both occupational workers and non - occupational population . -
pubmed
How the resulting
amino - acid substitutions impact
PrPC and
PrPSc structure and propagation is poorly understood . -
pubmed
Simultaneous measurement of salivary
cortisol and
alpha - amylase : Application and recommendations . -
pubmed
Salivary
cortisol ( sCort ) and salivary
alpha - amylase (
sAA ) constitute proxy measures of the two major stress response systems , i.e. -
pubmed
The combination of
BRAF inhibitor
dabrafenib with
MEK inhibitor
trametinib has shown its superiority to single agent therapy and is characterized by a tolerable spectrum of adverse events which shows a decrease in incidence over time on treatment . -
pubmed
Analysis of the 48 h absorption kinetics of
lutein showed that the time to peak level of
lutein ( 12 h ) was the same for SAF and
MDG groups , but the change in plasma
lutein at 12 and 48 h were 129 and 320 % higher , respectively , for
MDG compared with
SAF . -
pubmed
The strongest QTL was associated with flower color and localized to a SNP in the
anthocyanin biosynthesis pathway gene ,
dihydroflavonol-4 - reductase (
DFR ) . -
pubmed
PxylOR41 was tuned to two minor pheromone components Z9 - 14 : Ac , Z9 - 14 :
OH , and their analog Z9 - 14 :
Ald . -
pubmed
A 22 -
amino acid COOH - terminal cytoplasmic tail containing a potential site for phosphorylation by
protein kinase A was also found . -
pubmed
At long wavelengths the
Lewis acid complex is exclusively excited ; within the complex , one enantiotopic
face of the
aromatic aldehyde is efficiently shielded . -
pubmed
Curcumin (
diferuloylmethane ) , well known for its anti - inflammatory potential , was administered through intranasal route 1 h before LPS and OVA (
ovalbumin ) exposure to evaluate its efficacy against airway structural changes . -
pubmed
Hydroxyproline level and masson 's trichrome staining were used to evaluate
collagen deposition in lung . -
pubmed
Adenylyl cyclases (
ACs ) catalyze the formation of the second messenger
cAMP from
ATP . -
pubmed
Using an AC - specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N - terminus and demonstrate that
AtLRRAC1 can generate
cAMP in vitro . -
pubmed
Role of
Insulin - Mediated Antinatriuresis in
Sodium Homeostasis and
Hypertension . -
pubmed
Herein , the
BSR activities and
SMP generation / utilization were simultaneously investigated under different conditions , i.e. -
pubmed
Aromatase inhibitor and
tamoxifen use and the risk of
venous thromboembolism in
breast cancer survivors . -
pubmed
The aim of this study is to examine
VTE risk following long - term use of
aromatase inhibitor ( AI ) compared with
tamoxifen use among
breast cancer survivors . -
pubmed
Genetically predicted higher serum LA was associated with lower
diabetes ( odds ratio ( OR ) 0.97 per percentage in total
fatty acid increase in LA , 95 % confidence interval ( CI ) 0.96 to 0.99 ) and lower lipids (
low - density lipoprotein ,
high - density lipoprotein , and total
cholesterol ) , but may be associated with higher
diastolic blood pressure . -
pubmed
The molecular mass determined by
SDS - PAGE in the absence or presence of
beta - mercaptoethanol and ESI -
MS were 16.63 kDa and 18.362 kDa , respectively . -
pubmed
18F - DCFPyL PET /
CT scans were reviewed for sites of disease and compared to conventional imaging . -
pubmed
18F - DCFPyL PET /
CT detected 29 sites of
metastatic disease and 3 primary
tumors . -
pubmed
The detection rates of conventional imaging and
18F - DCFPyL PET /
CT for identifying sites of disease were 66.7 % and 88.9 % , respectively . -
pubmed
AIM : The aim of the present study was to compare and evaluate the flexural strength of heat - polymerized
Lucitone 199 and
SR Ivocap denture base resin materials which uses polymerization techniques of compression molding and injection molding respectively and effect of artificial saliva and distilled water on long - term . -
pubmed
The higher flexural strength was seen in
SR Ivocap compared to that of
Lucitone199 with the p value less than 0.05 which showed significant statistically . -
pubmed
In
overweight , the area under curve ( AUC ) for FT3 and postprandial
sugar showed 0.621 and 0.531 with cutoff values of 2.02 pg / ml and 147.5 mg / dl , respectively , whereas for
aspartate aminotransferase /
alanine aminotransferase ( De Ritis ratio ) , the AUC was 0.583 . -
pubmed
As regards ,
obese diabetics and the AUC for
insulin and homeostatic model assessment IR were 0.657 and 0.709 , respectively , with cutoff values of 16.06 mIU / L and 7.274 , respectively , and for postprandial
sugar 0.727 , in the same group (
obese ) with cutoff value of 208.5 mg / dl . -
pubmed
OBJECTIVE :
Tofacitinib is an oral
JAK inhibitor for the treatment of
rheumatoid arthritis (
RA ) . -
pubmed
In subsequent multivariable analyses , baseline age ,
hypertension , and the total
cholesterol to
high - density lipoprotein (
HDL )
cholesterol ratio remained significantly associated with risk of
MACE . -
pubmed
After 24 weeks of treatment , an increase in
HDL cholesterol and a decrease in the total to
HDL cholesterol were associated with decreased MACE risk ; changes in total
cholesterol ,
low - density lipoprotein (
LDL )
cholesterol , and disease activity measures were not . -
pubmed
CONCLUSION : In this
post hoc analysis , after 24 weeks of
tofacitinib treatment , increased
HDL cholesterol , but not increased
LDL cholesterol or total
cholesterol , appeared to be associated with lower future
MACE risk . -
pubmed
All doses of
galloyl - HHDP - glucose inhibited LPS - induced
JNK and
NF - kappaB activation . -
pubmed
Exclusion criteria were
allergy to
pregabalin ;
calcium channel blockers , antiepileptic drugs , antidepressant drugs , any analgesics , sedatives , or oral hypoglycemic agents . -
pubmed
Chronic treatment with
apelin ,
losartan and their combination reduces
myocardial infarct size and improves cardiac mechanical function . -
pubmed
Determination of four priority
polycyclic aromatic hydrocarbons (
4PAHs ) by
GC -
MS in traditional Turkish yoghurts . -
pubmed
Therefore , the concentration and types of four
polycyclic aromatic hydrocarbons were determined by gas chromatography - mass spectrometry (
GC -
MS ) . -
pubmed
Reversal of NOR deficits by systemic
S18841 was prevented by the
NMDA receptor antagonist ,
CPP ( 20 mg / kg ) , and the
glycine modulatory site antagonist ,
L701,324 ( 10 mg / kg ) . -
pubmed
The Impact of Aerobic Exercise and Badminton on
HDL Cholesterol Levels in Taiwanese Adults . -
pubmed
Elevated levels of
high - density lipoprotein cholesterol (
HDL - C ) have been associated with a decreased risk of
coronary heart disease (
CHD ) . -
pubmed
Mitochondrial mass and mitochondrial DNA ( mtDNA ) content were significantly increased with the upregulated expression of mitochondrial biogenesis regulator
PGC1 - A upon DE differentiation , accompanied by the rise of the amount of
ATP ( 2.5 - fold ) and its by - product reactive
oxygen species ( 2.0 - fold ) . -
pubmed
Here we analyzed the basal
CpG methylation of 11
KRAS - mutant and dependent
pancreatic cancer cell lines and observed strikingly similar methylation patterns . -
pubmed
This study presents the performance of a hybrid constructed wetland ( Bp(VF + HF)2:1 ) system which consists of an unsaturated vertical flow ( VF ) unit followed by a saturated down - flow unit simulating horizontal flow (
HF ) with
HF / VF area ratio of 0.5 and influent bypass to the
HF unit . -
pubmed
Treating synthetic wastewater simulating municipal wastewater , optimum total
nitrogen (
TN ) removal ( 57 % ) was reached at 39 % bypass and surface loading rate ( SLR ) of 33 g BOD5 / m2 day and 9.7 g
TN / m2 day ( overall system ) . -
pubmed
Overall greenhouse gas emissions were 0.11 (
N2O ) and 0.41 (
CH4 ) g / m2 day which corresponded to emissions factors ( relative to total organic
carbon and
TN influent ) of 0.7 % (
N2O ) and 3.6 % (
CH4 ) . -
pubmed
Compared with a similar system with a different
HF / VF area ratio of 2.0 , organic matter and
nitrogen removal efficiency was similar , but surface removal rates were about 3 times higher . -
pubmed
The results showed that the LPS - induced mammary histopathological changes and
MPO activity were attenuated by
piperine . -
pubmed
LPS - induced inflammatory
cytokines TNF - alpha andIL-1beta were also inhibited by
piperine . -
pubmed
Furthermore , LPS - induced
NF - kappaB activation was suppressed by the treatment with
piperine . -
pubmed
In addition , we found
piperine dose - dependently increased the expression of
PPARgamma . -
pubmed
All of these results suggested that
piperine had protective effects against LPS - induced
mastitis and that the mechanism may be mediated through the activation of
PPARgamma . -
pubmed
In this study , we identified a novel regulatory phosphorylation site (
Ser-262 ) on
OX1R that abolished its capability to interact with
GRK2 , but did not affect its interaction with G proteins ,
GRK5 , or
beta - arrestin1 / 2 activation , indicating that
Ser-262 is a key
amino acid for
OX1R internalization that contributes to induction of
GRK2 - dependent biased signaling via
orexin A. -
pubmed
P2X7 receptors become activated under pathological conditions of
ATP release like
hypoxia or cell destruction . -
pubmed
We found that
hP2X7 receptors become activated by
ATP within a few milliseconds and are permeable only to cations . -
pubmed
Petroleum - derived ( petro-)polymers such as
polyethylene (
PE ) ,
polyethylene terephthalate (
PET ) ,
polyurethane ( PU ) ,
polystyrene ( PS ) ,
polypropylene (
PP ) , and
polyvinyl chloride (
PVC ) are extremely recalcitrant to natural biodegradation pathways . -
pubmed
Among the several
alcohol dehydrogenases ,
PQQ - dependent enzymes are mainly found in the alpha , beta , and gamma - proteobacteria . -
pubmed
Type I ADHs are localized in the periplasm and contain one
Ca2 + - PQQ moiety , being the
methanol dehydrogenase (
MDH ) the most representative . -
pubmed
In recent years , several
lanthanide - dependent
MDHs have been discovered exploding the understanding of the natural role of
lanthanide ions . -
pubmed
Type II ADHs are localized in the periplasm and possess one
Ca2 + - PQQ moiety and one
heme c group . -
pubmed
Finally ,
type III ADHs are complexes of two or three subunits localized in the cytoplasmic membrane and possess one
Ca2 + - PQQ moiety and four
heme c groups , and in one of these proteins , an additional [
2Fe-2S ] cluster has been discovered recently . -
pubmed
From the bioinorganic point of view ,
PQQ - dependent alcohol dehydrogenases have been revived recently mainly due to the discovery of the
lanthanide - dependent enzymes . -
pubmed
Here , we review the three types of
PQQ - dependent
ADHs with special focus on their structural features and electron transfer processes . -
pubmed
ADH - I have a
Ca - PQQ or a
Ln - PQQ ,
ADH - II a
Ca - PQQ and one
heme - c and
ADH - III a
Ca - PQQ and four hemes - c. This review focuses on their structural features and electron transfer processes . -
pubmed
It is suggested that the effectiveness of
pyridostigmine pretreatment is due to the carbamoylation of a portion of the tissue
acetylcholinesterase , which protects it against irreversible inhibition by soman : after
poisoning spontaneous decarbamoylation produces sufficient free
acetylcholinesterase to restore normal function . -
pubmed
[ Role of
cyclic adenosine-3 ' , 5 '- monophosphate in the regulation of
histidase in the skin in the process of ontogeny ] . -
pubmed
Concentration of
urocaninic acid in skin correlated with the
histidase activity . -
pubmed
Content of
urocaninic acid in skin and the
histidase activity were altered under effect of administration of
cylic-3 ' , 5 '- AMP ,
dibutyryl cyclic-3 ' , 5 '- AMP ,
glucagon ,
sodium fluoride ,
theophylline ,
actinomycin D and
cycloheximide . -
pubmed
The plasma and pituitary
growth hormone and thyrotrophic hormone levels were also determined , as well as the thyroidal
127I content and the plasma
PBI . -
pubmed
Results are discussed in terms of the possible relationships between
thyroid hormone and
GH economy during a critical developmental period . -
pubmed
Immunoreactivity was greatly enhanced by pretreatment of tissue with
hyaluronidase , presumably due to removal of hyaluronan bound to the
HABP , and was abolished on treatment with hyaluronan
hexasaccharide , presumably due to inhibition of
HABP - antibody interaction . -
pubmed
Calcium - dependent cell - cell adhesion among embryonic chick neural retina cells is mediated by
N - cadherin , a 130,000 - Da integral membrane protein . -
pubmed
We have reported that the ability of chick neural retina cells to form
calcium - dependent cell - cell adhesion is also correlated with the presence of an
N - acetylgalactosaminyltransferase at the cell surface ( Balsamo , J. , and Lilien , J. -
pubmed
Antibodies prepared against
N - cadherin precipitate the
transferase containing complexes from neutral detergent extracts ; however , when the complexes are dissociated by treatment with
sodium dodecyl sulfate prior to immunoprecipitation , only
N - cadherin is precipitated . -
pubmed
Similarly ,
anti - transferase antibodies immunoprecipitate
N - cadherin containing complexes prior to disruption of the complex with
sodium dodecyl sulfate . -
pubmed
Catalysis of the
transferase reaction in the complex results in the transfer of
N - acetylgalactosamine phosphate to
oligosaccharide chains on
N - cadherin . -
pubmed
Glycosylation of
N - cadherin by the
transferase also occurs in situ ; labeling of retina cells with
[ 32P ] orthophosphate results in
N - cadherin molecules containing terminal
N - acetylgalactosamine linked to an
oligosaccharide chain via a
phosphodiester bond . -
pubmed
Initially , unoccupied
PR was isolated from cytosol extracts by
steroid affinity chromatography , followed by chromatography on
diethylaminoethyl Bio - Gel . -
pubmed
The partially pure ( 3 - 15 % )
PR consisted of two
steroid - binding components that migrated at 89 kDa and 109 kDa in reducing
sodium dodecyl sulfate gels after being photoaffinity labeled with the synthetic
progestin [
3H]R5020 . -
pubmed
Highly purified ( 30 - 70 % )
PR migrated as 93 kDa and 119 kDa
progestin - binding proteins in
sodium dodecyl sulfate gels . -
pubmed
Synaptosomes were labeled with
( 3H ) 5 - HT and superfused with physiological buffers containing selective concentrations of a type A (
clorgyline ) or a type B (
deprenyl )
MAO inhibitor . -
pubmed
In order to elevate cytoplasmic concentrations of
( 3H ) 5 - HT and therefore increase its chances for interaction with nerve terminal
MAO ,
reserpine was added to the superfusion buffer . -
pubmed
LHRH release is dependent on the availability of
calcium , and
prostaglandin E2 is a potent releaser of
LHRH . -
pubmed
PLA2 induced
LHRH release in a dose - related manner at amounts of 2 , 10 , and 50 U. Omission of
Ca ++ from the medium using
EGTA eliminated the
PLA2 effect . -
pubmed
Indomethacin treatment increased rather than diminished the
PLA2 stimulation . -
pubmed
These results are interpreted as a demonstration that
PLA2 has a role in the release of
LHRH and that a different route of the
cyclooxygenase may be involved besides the well known mediation of
prostaglandin E2 . -
pubmed
Allosteric
nucleotide specificity of
phosphorylase kinase : correlation of binding , conformational transitions , and activation . -
pubmed
Comparisons are made of the abilities of
5 '- diphosphate analogs of
ADP , including
phosphorothioates , to activate , to bind , and to induce in the
enzyme 's beta subunits conformational changes associated with activation . -
pubmed
Two of eight dysfunctional
C1 (-)- INH proteins were cleaved to lower mol wt forms that were not seen following the reaction of normal
C1 (-)- INH with equimolar amounts , or less , of
plasma kallikrein . -
pubmed
When a preparation of normal
C1 (-)- INH containing a homogeneous single band of
C1 (-)- INH was exposed to
C1 - s or
kallikrein , a " doublet " form evolved in which the heaviest band was in the original position of native
C1 (-)- INH ;
C1 - s cleavage provided a second band of 96,000 ; and cleavage by
kallikrein , a second band of 94,000 mol wt . -
pubmed
The doublet form of
C1 (-)- INH , which purified preparations frequently demonstrate , may be due to prior cleavage by
C1 - s or
kallikrein . -
pubmed
The effect of
RET on PG release required protein synthesis , inasmuch as it was lost in cultures maintained in media without
amino acids or in a low volume of media . -
pubmed
More
PG was released after
RET than after
IL-1 or LPS , and only
RET produced an effect that was evident by day 1 . -
pubmed
The amount of
CMP that produced the same size effect on PG release as these stimulators was below the detection level of
PG protease assays . -
pubmed
These inhibitors did not block another action of
RET on chondrocytes , namely the inhibition of
PG synthesis by
RET immediately after treatment . -
pubmed
The inhibitors did not act by reducing cell viability , because recovery of the rate of
PG synthesis 3 days
post - treatment occurred in inhibitor - treated cultures . -
pubmed
These studies suggest that
CMP is involved in cartilage
autolysis that is stimulated by
RET and
IL-1 . -
pubmed
Treatment of hepatocytes with
colcemid or other
microtubule - depolymerizing drugs reduced the half - life of
tubulin from 50 to 2 h , whereas
taxol , which stabilizes microtubules , increased the half - life . -
pubmed
Combination of
alkyl isonitriles with
cytochrome a. -
pubmed
A ' working ' model of the
haemoglobin molecule as a receptor for
2,3 - diphosphoglycerate . -
pubmed
The addition of
hydrocortisone and
insulin to the medium significantly shortens ( 25 + /- 2 PDL ) the lifespan of 10 day chick fibroblasts . -
pubmed
Hydrocortisone and
insulin have no effect on the growth kinetics or lifespan of retinal pigment cells in culture . -
pubmed
Modification of membrane
phospholipid structure can affect membrane viscosity , membrane - bound
enzyme activity , and the distribution and lateral mobility of integral proteins . -
pubmed
One hundred individual 1 - g doses of
cephalothin sodium in 50 - ml minibags were prepared by each of three methods ; the PharmAide Fluid Dispensing System ( PAFDS ) , the Valleylab
IV Formulator , and the Viavac vacuum unit , using 20 - g , 20 - g , and 1 - g vials of
cephalothin sodium , respectively . -
pubmed
Serum concentrations of total
cholesterol (
TC ) ,
triacylglycerol (
TAG ) ,
high density lipoprotein cholesterol (
HDL - C ) and the subfraction
HDL2 - C free
fatty acids (
FFA ) and free
glycerol were measured in venous blood samples drawn before exercise ( after a 12 - h fast ) , during walking and after 1 h and 24 h of recovery . -
pubmed
Analytical methods were developed and validated for the determination of
enloplatin ( an anticancer agent ) in plasma by reversed - phase LC and for
platinum ( an elemental component of
enloplatin ) in plasma , plasma ultrafiltrate (
PUF ) and whole blood by flameless atomic absorption spectrometry ( FAAS ) . -
pubmed
A
C18 or a
cyano column was used , depending on sample matrix , with UV detection at 230 nm . -
pubmed
An aliquot of plasma ,
PUF , or whole blood was mixed with a solution of
Triton X-100 and Antifoam - B and injected into the FAAS system . -
pubmed
Synthesis and structure - activity relationship of
methyl - substituted indolo [ 2,3 - b ] quinolines : novel cytotoxic ,
DNA topoisomerase II inhibitors . -
pubmed
In furtherance of our
SAR study on the chemistry and antitumor activity of fused
nitrogen heteroaromatic compounds , a series of linear ,
methyl - substituted derivatives of 5H- and
6H - indolo [ 2,3 - b ] quinolines were synthesized according to the modified Graebe - Ullmann reaction . -
pubmed
To establish the relationship between the physicochemical and biological activities of
indolo [ 2,3 - b ] quinolines , their lipophilic properties , cytotoxic and antimicrobial activity , and ability to induce
topoisomerase II dependent
pSP65 DNA cleavage in vitro were investigated . -
pubmed
Disulfiram (
DS , 0.50 - 2.00 mmol / kg ) , a corresponding
disulfide form , also exhibited similar protection . -
pubmed
The maximum effect of
DEDC was found by dosing at 0.5 hr prior to
alloxan , and the effect afforded by
DEDC pretreatment persisted up to 3 hr , whereas the effect of
DS was exhibited when the compound was given 0.5 hr prior to
alloxan . -
pubmed
At 0.5 hr after injection ,
DEDC alone had a potent increasing ability on blood
glucose in a dose - dependent manner , but
DS was less potent . -
pubmed
BW 284C51 and
ethopropazine , inhibiting
acetylcholinesterase (
AChE ) and
butyrylcholinesterase (
BChE ) , respectively , strongly decrease neurite growth in a dose - dependent manner . -
pubmed
However ,
echothiophate which inhibits both
cholinesterases , does not change neuritic growth . -
pubmed
Uronic - acid - rich protein (
UAP ) is a new urinary macromolecule which strongly inhibits
calcium oxalate crystal formation . -
pubmed
Glycosylphosphatidylinositol (
GPI)-anchored proteins are generally absent from the surface of cells that are defective in
GPI biosynthesis . -
pubmed
An
aspartic acid residue (
Asp316 ) within the Q7b
GPI anchoring signal , previously found to be essential for
GPI anchor addition ( Waneck , G. L. , Stein , M. E. , and Flavell , R. A. -
pubmed
Analysis by sedimentation on
sucrose gradients shows that Q7b species retained in the
ER are multimeric , whereas species that exit the
ER are monomeric . -
pubmed
Modulation of the higher - order folding of chromatin by deletion of
histone H3 and
H4 terminal domains . -
pubmed
The ' tails ' of
histones H3 and
H4 were removed by light in situ
trypsin digestion of the nuclei . -
pubmed
Phosphorylation of
Ga14p at a single C - terminal residue is necessary for
galactose - inducible transcription . -
pubmed
In this study , we show that phosphorylation occurs rapidly on a limited fraction of overexpressed
Gal4p present in a
sodium dodecyl sulfate - extractable subcellular fraction while a significant fraction remains stably unphosphorylated . -
pubmed
We demonstrate that
Gal4p is multiply phosphorylated at both the C and N termini , and we identify the precise locations of three sites of phosphorylation at
serines 691 , 696 , and 699 . -
pubmed
Serine 699 phosphorylation requires
Gal4p 's DNA binding function and is influenced by the function of the
RNA polymerase II holoenzyme component
Gal11p . -
pubmed
We have recently found that the
deoxynojirimycin - derivative
emiglitate , a recognized inhibitor of intestinal
alpha - glucosidehydrolase activity , is a powerful inhibitor of
glucose - induced
insulin release . -
pubmed
Treatment of freshly isolated islets with
pertussis toxin (
PTX ) , which is known to inactivate the
G ( i )- proteins , abolished the inhibitory effect of the
alpha ( 2 )- adrenoceptor agonist
clonidine on
insulin release stimulated by the
phosphodiesterase inhibitor
IBMX in the presence of the
protein kinase C activator
TPA and even changed it into an increase . -
pubmed
Emiglitate did not display any inhibitory action on
insulin release induced by these secretagogues . -
pubmed
Similarly ,
clonidine - induced inhibition of
glucose stimulated
insulin release was reversed by
PTX . -
pubmed
However ,
PTX did not influence the suppressive action of
emiglitate on
glucose - induced
insulin secretion . -
pubmed
In contrast , the
adenylate cyclase activator
forskolin totally abolished the inhibitory effect of
emiglitate , but not that of the
glucose analogue
mannoheptulose , on
glucose - induced
insulin release . -
pubmed
Moreover , the stimulatory effect of
forskolin and
cholera toxin (
CTX ) ( activator of
G ( s )- proteins ) on the secretion of
insulin was markedly enhanced in the presence of emiglitate . -
pubmed
In conclusion , our results suggest that the inhibitory effect of
emiglitate on
glucose - induced
insulin release is not directly related to the
G ( s )- proteins , but most likely exerted solely through the selective suppression of
lysosomal aglucosidehydrolase activity , a step in between the proximal and the distal
G ( i )- proteins , in
glucose induced stimulus - secretion mechanisms . -
pubmed
Our data also suggests that the inhibitory action of
emiglitate on
glucose stimulated
insulin release can be compensated for by an increased sensitivity of the
cyclic AMP - protein kinase A pathway . -
pubmed
Hence ,
emiglitate might indirectly elicit an increased activity of the
G ( s )- proteins to facilitate the secretory process . -
pubmed
To obtain evidence that
mAbs in each of these preliminary clusters detected the same
antigen , they were tested for their capacity to compete with each other for binding to a target cell and the molecular weights of their
antigens were estimated after immunoprecipitation . -
pubmed
Most preliminary clusters in the non - lineage panel contained control mAbs that had been characterised in one of the previous workshops , and this study therefore increased the number of
mAbs available to each of these non - lineage
antigens . -
pubmed
An additional cluster , which did not fit into any other panel , contained
mAbs specific for an
antigen restricted to the erythroid lineage and received the nomenclature BoWC15 ( ANA8 , IL -
A135 , IL - A137 , IL - A138 and
IL - A160 ) . -
pubmed
Subplantar injection of the non isoform- selective inhibitors of
nitric oxide ( NO ) synthase ,
N ( G )- nitro - L - Arginine methyl ester and
N ( G )- methyl - L - Arginine , and of a scavenger of
NO ,
haemoglobin , inhibited the early phase of
oedema development . -
pubmed
Inhibition of
cyclooxygenase activity by
indomethacin reduced the late increase in paw volume after the injection of zymosan - activated plasma . -
pubmed
Methylene blue , an inhibitor of the soluble
guanylate cyclase , had no effect . -
pubmed
Suppression of plasma
cholesteryl ester transfer protein activity in acute
hyperinsulinemia and effect of plasma nonesterified
fatty acid . -
pubmed
Cholesteryl ester transfer protein (
CETP ) is a major determinant of the plasma
high - density lipoprotein cholesterol ( HDL - C ) level and plays an important role in the reverse
cholesterol transport system . -
pubmed
CETP activity was determined as the transfer of
radiolabeled cholesterol in
HDL3 to acceptor lipoprotein . -
pubmed
Regression analysis showed that this suppression was correlated with plasma nonesterified
fatty acid (
NEFA ) levels after the
clamp and with the magnitude of the
NEFA decrease ( r = .318 , P & lt ; .02 and r = .292 , P & lt ; .05 , respectively ) . -
pubmed
The crystal structure of recombinant
enzyme complexed with
beta - cyclodextrin was refined at 2 . -
pubmed
[
Nitric oxide and
hemoglobin ] . -
pubmed
Nitric oxide (
NO ) is produced by
nitric oxide synthases (
cNOS and
iNOS ) in endothelial cells upon stimulation by various agents like
Ca ( 2 +)-calmodulin ,
cytokines and
TNF . -
pubmed
It acts as a paracrine on adjacent cells to activate soluble
guanylyl cyclase in the production of
cGMP , a second messenger in signal transduction cascades , leading to various cellular responses . -
pubmed
This homeostasis of
NO in the rapidly moving blood must be maintained by a delicate balance between its production by
NOSs and its instant scavenging by
hemoglobin (
Hb ) in the erythrocytes . -
pubmed
Upon binding
NO to the alpha - subunbits ,
Hb shifts its conformation to a T-(low - affinity extreme ) state and its beta - subunits become an efficient
O2 carrier . -
pubmed
The same molecular mechanism of
NO - induced conformation change operates in both
Hb and soluble
guanylyl cyclase . -
pubmed
This bond cleavage mechanism allows
Hb to survive as an effective
O2 carrier even after sequestration of
NO . -
pubmed
The
NO sequestered in
Hb is eventually oxidized aerobically to
NO3 - in the reaction of
Fe - NO +
O2 - ->
Fe (+ ) +
NO3 . -
pubmed
Met Hb (
Fe + ) so formed is cycled back to
deoxy Hb (
Fe ) by
intra - erythrocyte Hb reductase to complete the
NO scavenging . -
pubmed
Thus , the
NO in the blood acts on soluble
guanylyl cyclase in vascular smooth muscles to dilate the blood vessels to increase blood delivery , whereas excess
NO in the blood , which is sequestered by
Hb , could help
Hb to deliver
O2 more efficiently in peripheral tissues . -
pubmed
This was consistent with removal of the signal peptide from the
chitinase and an
ER localization motif ( KDEL ) at the
carboxyl end of the protein . -
pubmed
At the end of the experiment , mean arterial pressure ( MAP ) and heart rate at
rest and in response to ganglionic blockade by intravenous
hexamethonium ( 30 mg / kg ) were assessed . -
pubmed
pH gradients in immobilized
amidases and their influence on rates and yields of
beta - lactam hydrolysis . -
pubmed
Studies in neuronal cells and other cells have shown that
ethanol can alter intracellular
calcium levels and affect
voltage and receptor - operated calcium channels , as well as
G protein - mediated
calcium responses . -
pubmed
UTP and
UDP ( 1 - 500 microM ) were equipotent in inducing concentration - dependent vasoconstriction , unaffected by the
P2 receptor antagonists
suramin ( 100 microM ) and
Reactive blue 2 ( 50 microM ) ;
ATP ( 10 - 500 microM ) produced weaker vasoconstriction . -
pubmed
Furthermore , the pharmacological profile of the native
UTP / UDP receptor differs from that of the known
P2Y2 ,
P2Y4 and
P2Y6 recombinant receptors for
pyrimidine nucleotides . -
pubmed
METHODS :
Mucins were extracted in a denaturing medium ( to prevent endogenous proteolysis ) and purified by
caesium chloride density gradient ultracentrifugation . -
pubmed
Analysis of
mucin was by gel filtration ,
SDS - PAGE and Western blotting methods . -
pubmed
We identified by
SDS - PAGE a glycoprotein which co - fractionates in a
caesium chloride density gradient with mucins isolated from gastrectomy specimens resected for
carcinoma and
peptic ulceration and which was absent from
mucins of the transplant donor control group . -
pubmed
This
neuraminidase - sensitive glycoprotein resisted dissociation from
mucin during purification in a 3.5 M
CsCl density gradient but was partially separable by Sepharose 2B gel chromatography and heat treatment ( 100 degrees C , 2.0 min ) in
SDS . -
pubmed
Dextromethorphan ( DM ) is metabolized in the body to
dextrophan (
DT ) and
3 - methoxymorphinan ( 3 - MM ) by
cytochrome P450 ( CYP ) 2D6 and
3A4 , respectively , and
cyclosporine (
CsA ) is a known substrate of
CYP3A4 . -
pubmed
Metabolic ratios of DM:3 - MM based on 24 - hour cumulative urine collection did not appear to have clinical utility in predicting
CYP3A activity measured by
CsA clearance . -
pubmed
Selection in the presence of either the herbicide
Basta or the
adenosine analogue
2 '- deoxyadenosine resulted in transgenic cultures that expressed
GUS and accumulated a 41 - kD protein that immunoprecipated with an
ADA - specific polyclonal antibody . -
pubmed
Culltures expressing
ADA grew in the presence of 200 mg / l
2 '- deoxyadenosine , a concentration which completely inhibited the growth of non - transgenic cultures . -
pubmed
This epitope loss was observed following either direct stimulation of
protein kinase C (
PKC ) with
phorbol 12 - myristate 13 - acetate (
PMA ) or after multiple receptor stimulation using a combination of the agonist
N - formylmethionyl - leucyl - phenylalanine (
FMLP ) and the priming agents
granulocyte macrophage - colony stimulating factor (
GM - CSF ) and
platelet factor ( PF ) 4 . -
pubmed
The increases and subsequent decreases in
CD11b expression induced by
PMA were paralleled by an increase and a decrease in PMN adhesion to
CD11b - specific ligands ,
fibrinogen and
intercellular adhesion molecule ( ICAM )- 1 . -
pubmed
Sodium dodecyl sulphate -
polyacrylamide gel electrophoresis (
SDS - PAGE ) and Western blot analysis showed that this epitope loss of
PMN CD11b was the result of proteolytic degradation of the
N - terminal region of the molecule . -
pubmed
The exogenous
IL-2 completely restored the inhibitory effect of
xylazine in vivo on thymocyte proliferation . -
pubmed
However , a minimal influence of the
cytokine on the
xylazine - inhibited thymocyte proliferation in vitro was observed . -
pubmed
Stimulatory effect of
xylazine on proliferation of thymocytes was probably mediated through
alpha 2 - adrenoreceptors since it was blocked by
yohimbine , an
alpha 2 - adrenoreceptor antagonist . -
pubmed
In material stained with the
post - embedding immunogold method , approximately one - third of plasma membrane - bound gold particles labeling in axon terminals and spines is associated with asymmetric synapses , thereby representing synaptic
kainate receptor subunits . -
pubmed
In conclusion , these data demonstrate that
kainate receptors in the striatum display a pattern of subcellular distribution different from other ionotropic
glutamate receptor subtypes , but consistent with their metabotropic - like functions recently shown in the hippocampus . -
pubmed
The 3 ' portion of the
VIT1 message is complementary to the 3 ' end of
hMSH6 mRNA , enabling the formation of RNA - RNA hybrids , which may interfere with G / T mismatch repair function . -
pubmed
These results suggest that
RNH-6270 is a potent
AT1 receptor antagonist and has anti - proliferative effects on VSMCs from SHR , which was not seen with an
ACE inhibitor . -
pubmed
The growth inhibitory effect of
RNH-6270 may be associated with the inhibition of growth factors via antagonism to
AT1 receptors . -
pubmed
Control of
oxygen free radical formation from mitochondrial complex I : roles for
protein kinase A and
pyruvate dehydrogenase kinase . -
pubmed
Phosphorylation of both subunits was enhanced by
cAMP derivatives and
protein kinase A (
PKA ) and was inhibited by
PKA inhibitors ( PKAi ) . -
pubmed
NADH cytochrome c reductase activity was decreased and
superoxide production rates with
NADH as substrate were increased . -
pubmed
On the other hand , with
protein kinase A - driven phosphorylation ,
NADH cytochrome c reductase was increased and
superoxide production decreased . -
pubmed
In light of these observations we discuss a potential model for the dual regulation of
complex I and the production of
oxygen free radicals by both
PKA and
PDH kinase . -
pubmed
In the RR spectra , the
nu ( Fe - CO ) bands were observed at 506 , 489 cm(-1 ) ( TCP ) , 465 cm(-1 ) ( TCP - PY ) , 458 , 437 cm(-1 ) ( TCP -
TG ) and 429 cm(-1 ) ( TCP - TB ) . -
pubmed
Compared with the reported
nu ( Fe - CO ) frequencies of
hemoproteins and their model systems , these observed values are unusually low . -
pubmed
This prediction is further supported by the observation of significant
13C shieldings exhibited by
TCP - CO ( delta = 202.6 ppm ) and
TCP - PY - CO ( delta = 202.3 ppm ) , in comparison to
hemoproteins and other
heme models . -
pubmed
We examined the role of histone deacetylation in
cyclin Bl regulation using the
histone deacetylase inhibitor
trichostatin A (
TSA ) . -
pubmed
Promoter activity increased only 2 - fold following
TSA treatment of G(0 ) cells containing the construct pCycB(MUT - E - Box)-
LUC with an E - box mutation , and a decrease in activity was detected during G(2 ) . -
pubmed
TSA reduction of
cyclin B1 promoter activity in G(2 ) , however , involves sequences within the first 119 bp . -
pubmed
The native cholinergic receptor that mediates synaptic transmission between olivocochlear fibers and outer hair cells of the cochlea is permeable to
Ca ( 2 + ) and is thought to be composed of both the
alpha 9 and the alpha 10 cholinergic nicotinic subunits . -
pubmed
The aim of the present work was to study the permeability of the recombinant
alpha 9 alpha 10 nicotinic acetylcholine receptor to
Ca ( 2 + ) ,
Ba ( 2 + ) and
Mg ( 2 + ) and its modulation by these divalent cations . -
pubmed
In the absence of
Ca ( 2 + ) , the EC(50 ) for
ACh was higher ( 48 microM ) than that obtained with 1.8 mM
Ca ( 2 + ) ( 14.3 microM ) , suggesting that potentiation by
Ca ( 2 + ) involves changes in the apparent affinity of the
alpha 9 alpha 10 receptor for
ACh . -
pubmed
We assessed the methylation status of the
CpG islands in the promoter region of the
p73 gene by methylation - specific PCR in 117
glioma biopsies . -
pubmed
The nuclear localization signal ( NLS ) sequence of
topo II is a non - classical bipartite type containing two interdependent , positively charged clusters separated by 15
amino acids . -
pubmed
Serum
paraoxonase (
PON ) is a high - density lipoprotein - bound enzyme that can prevent oxidation of low - density lipoprotein by hydrolyzing lipid
peroxides and thus exert an anti - atherogenic effect . -
pubmed
Recent studies have suggested that
glutamine(Q isoform)/
arginine(R isoform ) polymorphism at position 192 of
PON ( 1 ) gene is associated with
macrovascular disease of
type 2 diabetes mellitus (
T2DM ) . -
pubmed
[ Critical survey of
cortisone and
ACTH therapy of
bronchial asthma ] . -
pubmed
Objectives in this study were : ( 1 ) to describe the effects of
adrenomedullin and anti -
adrenomedullin antibodies on
ovarian carcinoma cell proliferation , and ( 2 ) to assess the
estradiol regulation of
adrenomedullin metabolism . -
pubmed
Adrenomedullin mRNA expression and secretion were not under
estrogen control . -
pubmed
CONCLUSION :
Adrenomedullin metabolism is not under
estradiol control . -
pubmed
Our results show that
NTN expression is relatively low in the striatum , the target of
dopamine ( DA ) neurons , and there is no apparent pattern of developmental regulation in
SN . -
pubmed
Polymorphisms in the
methylenetetrahydrofolate reductase and
methionine synthase reductase genes and
homocysteine levels in Brazilian children . -
pubmed
Hyperhomocysteinemia is a risk factor for
thrombosis , and
methylenetetrahydrofolate reductase (
MTHFR ) and
methionine synthase reductase (
MTRR ) polymorphisms ,
folate , and B12 levels could contribute to plasma
homocysteine (
Hcy ) variation . -
pubmed
In this study , we investigated association of polymorphisms C677 T and A1298C in the
MTHFR gene and A66 G in the
MTRR gene with
Hcy levels in children . -
pubmed
The
folate levels demonstrated a statistically significant decrease ( P = 0.0477 ) from the C677 T mutation in the
MTHFR gene ( TT genotype ) when compared to the other groups . -
pubmed
CRP peak was lower with
statins ( 3.8 versus 7.0 mg / dl ; p = 0.0446 ) . -
pubmed
Fibrates were only associated with lower
CRP levels at admission ( 0.6 versus 0.9 mg / dl ; p = 0.0010 ) . -
pubmed
The effect of
statins and
fibrates on
CRP levels in
AMI should be studied further . -
pubmed
Here , we use both in vivo and in vitro studies to investigate the potential roles of two prominent factors ,
vascular endothelial growth factor (
VEGF ) and
brain - derived neurotrophic factor (
BDNF ) , in mediating brain plasticity after treatment of
stroke with
atorvastatin . -
pubmed
Atorvastatin increases
VEGF ,
VEGFR2 and
BDNF expression in the ischemic border . -
pubmed
Numbers of migrating neurons , developmental neurons and
synaptophysin - positive cells as well as indices of angiogenesis were significantly increased in the
atorvastatin treatment group , compared with controls . -
pubmed
Anti -
BDNF antibody significantly inhibited
atorvastatin - induced SVZ explant cell migration , indicating a prominent role for
BDNF in progenitor cell migration . -
pubmed
Antioxidant status was evaluated by measuring the red cell
superoxide dismutase and
catalase activity , total blood
glutathione ,
glutathione peroxidase activity in red cell and plasma , and total antioxidant capacity ( assessed as
ferric reducing antioxidant power ) in plasma . -
pubmed
No significant differences were found in plasma
glutathione peroxidase , protein
carbonyls , and total
nitrates and
nitrites , red cell antioxidative enzyme activities ,
superoxide anion release from leukocytes , and total blood
glutathione between the two groups ( p & gt ; 0.05 ) . -
pubmed
Subsequent experiments have shown that
TEC - tolerance is dominant and mediated by
CD4 regulatory T cells ( Treg ) that are generated intrathymically by recognition of
antigens expressed on TECs ( Modigliani et al . -
pubmed
1996b ) , with two central propositions : ( 1 )
T cell receptor (
TCR)-dependent sorting of emergent repertoires generates
TEC - specific Treg displaying the highest
TCR self - affinities below deletion thresholds , thus isolating repertoires undergoing positive and negative selection ; ( 2 ) Treg are intrathymically committed ( and activated ) for a unique differentiative pathway with regulatory
effector functions . -
pubmed
2003 ) , which is adopted by
CD4 T cells with high avidity for
TEC -
antigens ( Bensinger et al . -
pubmed
1996a ) might add to this process , it would seem that the establishment of tissue - specific tolerance essentially stems from the " promiscuous expression of tissue
antigens " by
TEC . -
pubmed
The first targeted therapy for
lung cancer is
gefitinib , an
EGFR inhibitor , which was approved in several countries in 2003 . -
pubmed
Decoy
androgen - responsive element DNA can inhibit
androgen receptor transactivation of the
PSA promoter gene . -
pubmed
Androgen receptors (
ARs ) in the cytoplasm are activated by binding with
androgen . -
pubmed
Androgen - activated
ARs bind to a specific genomic DNA sequence , the
androgen - responsive element ( ARE ) , and initiate gene expression at the transcriptional level . -
pubmed
Even without
androgen activation ,
ARs may have a role in
androgen - refractory prostate cancer . -
pubmed
After 24 hr exposure to
androgens , the cells were lysed and
luciferase activity measured to determine the ARE decoy inhibitory effect on the function of
ARs . -
pubmed
The results demonstrate that ARE decoy DNA can effectively suppress
androgen - activated
ARs in
prostate cancer cells and indicate the potential utility of decoy DNA for developing a novel therapy for
prostate cancer . -
pubmed
The extensive toxin :
ARF -
GTP interface surface mimics
ARF -
GTP recognition of normal cellular protein partners , which suggests that the toxin has evolved to exploit promiscuous binding properties of
ARFs . -
pubmed
Five months after surgery , combination chemotherapy with
TS-1 ( 100 mg / body / day , 3 weeks ) and
CDDP ( 10 mg / body / day , day 1 , 8 , 15 drip infusion ) in 1 course was performed , and complete response (
CR ) was noted . -
pubmed
In all cases , ( a ) intestinal
metaplasia ( IM ) extension ( H&E ) , ( b ) IM phenotype ( high -
iron diamine [ HID ] ) , and ( c )
Cdx2 immunohistochemical expression were histologically scored in the biopsy material obtained before and after fundoplication . -
pubmed
The fraction sedimenting at 1,200 g comprised mostly intact
chloroplasts and contained more than half the
chlorophyll but only 5 % of the
3 - phosphoglycerate phosphatase activity present in the homogenate . -
pubmed
Further purification of the 1,200 g pellet fraction on a Percoll step gradient yielded greater than 95 % intact
chloroplasts , yet the
phosphatase activity was reduced more than 15 - fold on a
chlorophyll basis by this purification . -
pubmed
When the intact
chloroplast and cytoplasmic fractions of mesophyll protoplasts were separated by
silicone oil filtering centrifugation , the
chloroplast fraction contained more than 90 % of the
chlorophyll but had less than 12 % of the
3 - phosphoglycerate phosphatase activity . -
pubmed
Pancreatic blood flow was detected by intravital microscope ;
malondialdehyde (
MDA ) content and
superoxide dismutase (
SOD ) activity were measured in pancreatic tissues for assessment of oxidative stress and
alpha - smooth muscle actin (
alpha - SMA ) expression was determined by immunohistochemical staining and RT - PCR . -
pubmed
Zn ( II ) in activating concentrations ( 100 microM ) acted as an allosteric enhancer as it increased the B(max ) ( 7.1 - fold ) , the potency ( 9.9 - fold ) , the affinity ( 1.7- and 6.1 - fold in competition against agonist and inverse agonist , respectively ) , and the efficacy ( 2.5 - fold ) of
CXCL1 /
GROalpha . -
pubmed
The activating properties of
Zn ( II ) were not due to a metal ion site between the ligand and the receptor because
CXCL1 /
GROalpha analogs in which the putative metal - ion binding residues had been substituted-[H19A ] and
[ H34A ]-acted like wild - type
CXCL1 /
GROalpha . -
pubmed
Based on the complex action of
Zn ( II ) and on the
chemokine interaction for [ R208H;R212H]-
ORF74 , we conclude that the extracellular end of
TM - V is important for the activation of this
CXC - chemokine receptor . -
pubmed
CONCLUSIONS :
ALA - PDT was shown to be an effective and safe therapy for the treatment of
AKs of the
face and scalp in skin types I - V , with an acceptable rate of
recurrence over 12 months of histologically confirmed
AKs of 19 % . -
pubmed
Leptin mRNA expression levels in the subcutaneous and omental adipose tissues were measured using
SYBR Green I quantitative real - time PCR . -
pubmed
Strategies of replacing
protease inhibitors with
nonnucleoside reverse transcriptase inhibitors or
abacavir have been shown to improve
metabolic abnormalities , particularly by decreasing
cholesterol and
triglyceride levels , and reducing cardiovascular risk . -
pubmed
Nonnucleoside reverse transcriptase inhibitors , particularly
nevirapine , result in a better lipid profile with a greater increase in HDL
cholesterol and in the HDUtotal
cholesterol ratio , one of the most important parameters associated with a reduction in cardiovascular risk . -
pubmed
Although
protease inhibitor compounds as a family have been linked to metabolic and body fat alterations , new drugs such as
atazanavir seem to be associated with a more favorable lipid profile . -
pubmed
Macrolide antibiotics , such as
clarithromycin , have in vitro efficacy against
IL-8 and neutrophils , key inflammatory mediators in
noneosinophilic asthma . -
pubmed
Clarithromycin therapy significantly reduced airway concentrations of
IL-8 and neutrophil numbers and improved quality - of - life scores compared with placebo . -
pubmed
From the atoms in molecules (
AIM ) theoretical analysis , common electronic features for the planar and puckered
cyclobutenyl dication derivatives were observed . -
pubmed
Transcription of many genes including
GDNF is directed by the
cAMP responsive element ( CRE ) and its cognate transcription factor
CRE binding protein (
CREB ) . -
pubmed
Different classes of antidepressants as well as
amitriptyline acutely increased phosphorylation of
CREB , but
haloperidol and
diazepam did not . -
pubmed
The
amitriptyline - induced phosphorylation of
CREB was completely blocked by
U0126 [ a
mitogen - activated protein ( MAP ) kinase kinase 1 inhibitor ] and
genistein ( a
PTK inhibitor ) , but not by inhibitors of
protein kinase A ,
p38 MAP kinase , or
Ca ( 2 +)/ calmodulin - dependent kinase . -
pubmed
Amitriptyline treatment also increased the expression of
luciferase reporter gene regulated by CRE elements . -
pubmed
The
amitriptyline - induced
luciferase activity was completely inhibited by
U0126 in the same as phosphorylation of
CREB . -
pubmed
[ Carbo - dehydrated
transferrin (
CDT ) as a marker of
alcohol intake : problems with analysis and interpretation . -
pubmed
Apigenin - mediated dephosphorylation of
Akt resulted in inhibition of its
kinase activity , which was confirmed by reduced phosphorylation of proapoptotic proteins
BAD and
glycogen synthase kinase-3 , essential downstream targets of
Akt . -
pubmed
These results suggest that
Akt inactivation and dephosphorylation of
BAD is a critical event , at least in part , in
apigenin - induced decreased cell survival and apoptosis . -
pubmed
5 - Lipoxygenase inhibitors potentiate
1alpha,25 - dihydroxyvitamin D3 - induced monocytic differentiation by activating
p38 MAPK pathway . -
pubmed
The inhibition of differentiation by SB was demonstrated by a reduction of
CD14 expression and by a decrease in
OZP - activated ROS production . -
pubmed
The levels of total
cholesterol ,
high - density lipoprotein cholesterol ,
triglyceride and
homocysteine were measured . -
pubmed
Total
cholesterol was decreased , while
high - density lipoprotein cholesterol was increased in heparin compared with that in control group ( P=0.001 and 0.002 ) . -
pubmed
Coincidently , the expression of
MMP-2 in the
atherosclerotic lesions in the
heparin group was 49.3 % lower than that in the control group ( P<0.001 ) . -
pubmed
Only high activity of
gastritis ( neutrophil infiltration ) and low bacterial load in gastric mucosal specimens as well as drinking
alcohol and the use of
histamine H2 receptor blockers reduced culture efficacy in infected subjects . -
pubmed
On the self - assembly of a highly selective
benzothiazole - based
TIM inhibitor in aqueous solution . -
pubmed
In these cells ,
estradiol ( E(2 ) ) /
ER and synthetic
androgen (
R1881)/
AR trans - activate
EGFR leading to the downstream signaling and to
ERK activation . -
pubmed
Structurally ,
FI is a 88kDa heterodimer of a heavy chain consisting of one
FI - membrane attack complex ( FIMAC ) domain , one
CD5 domain and two
low - density lipoprotein receptor domains (
LDLr ) , and a light chain which is a
serine protease domain ( SP ) , linked to the heavy chain by a
disulfide bond . -
pubmed
The pathophysiology of
iron - induced compromised fertility in women with
thalassemia major (
TM ) was evaluated in 26 adult
TM females . -
pubmed
AMH correlated with non -
transferrin - bound
iron ( NTBI ) , suggesting a role of labile
iron in the pathogenesis of
decreased reproductive capacity , possibly occurring in parallel to
cardiac iron toxicity , as cardiac
iron was associated with the presence of
amenorrhea and with NTBI levels . -
pubmed
The optimal conditions for the
AR induction and modulation were determined as : 2 h - intertreatment time ( in
PBS , at 4 C ) given after a priming dose ( 50 microg / ml ) of
zeocin treatment . -
pubmed
These extracts enhanced the
AR and also decreased DNA damage caused by
zeocin ( after 0 , 1 and 4 h - recovery time after the test dose of
zeocin application ) to more than 50 % . -
pubmed
Sublethal LPS transiently increased the expression of critical components of the mitochondrial transcriptional machinery , including
nuclear respiratory factor 1 (
NRF1 ) and
mitochondrial transcription factor A (
TFAM ) , as well as mtDNA copy number , mitochondrial protein levels , and markers of functional mitochondria , such as increased cellular
ATP content ,
citrate synthase activity , and maximal respiration capacity . -
pubmed
Development of cross - reactive antibodies to the
proline - rich region of
pneumococcal surface protein A in children . -
pubmed
In this study , sera of children with history of
pneumococcal colonization were analyzed for presence of
IgG antibodies to the conserved
proline - rich region (
PRR ) of
PspA. -
pubmed
A new bifunctional compound
NCC , which undergoes
thiol - mediated
disulfide cleavage after cell entry , produces a red - shifted fluorescent emission in the cytosol and releases free active DNA alkylating agent
CLB into the nucleus , and finally leads to DNA damage and cell
death . -
pubmed
Serum free
triiodothyronine and free
thyroxine levels were elevated and
TSH level was suppressed , suggesting the existence of
hyperthyroidism . -
pubmed
These results suggest that
CS exposure initiates an
Ag - specific response that leads to pulmonary destruction and
inflammation that involves both
CD8(+ ) and
CD4(+ ) T cells . -
pubmed
The results showed that
IdeR binds 2 equiv of
Fe ( II ) with dissociation constants that differ by a factor of 25 . -
pubmed
The high- and low - affinity
iron binding sites were assigned to physical binding sites I and
II , respectively , using metal binding site mutants . -
pubmed
IdeR was also found to contain a high - affinity
Zn ( II ) binding site that was assigned to physical metal binding site
II through the use of binding site mutants and metal competition assays . -
pubmed
Together , these results suggest that
IdeR is a mixed - metal repressor , where
Zn ( II ) acts as a structural metal and
Fe ( II ) acts to trigger the physiologically relevant promoter binding . -
pubmed
CONCLUSIONS : We concluded that the atlantoaxial transarticular screw fixation and posterior fusion using
polyester cable can be used for
C1 - 2 fusion with a high fusion rate and less complications in various cases . -
pubmed
The
antithrombin (
AT ) binding properties of heparin and low molecular weight heparins are strongly associated to the presence of the
pentasaccharide sequence
AGA * IA ( A ( NAc,6S )- GlcUA - A ( NS,3,6S )- I ( 2S )- A ( NS,6S ) ) . -
pubmed
This novel
oligosaccharide was characterized by NMR spectroscopy , and its binding with
AT was determined by fluorescence titration , NMR , and LC -
MS . -
pubmed
These results establish alterations of the gene encoding the
NMDA receptor
NR2A subunit as a major genetic risk factor for
IFE . -
pubmed
Synthesis of novel
pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine derivatives : potent and selective
adenosine A3 receptor antagonists . -
pubmed
Compounds
2 -( 3 - fluorophenyl )- 8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5b ) ,
2 -( furan-2 - yl )- 8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5d ) , and
2 -( furan-2 - yl )- 5 - methyl-8 - phenyl-10 -( trifluoromethyl ) pyrido [ 3,2 - e ][ 1,2,4 ] triazolo [ 1,5 - c ] pyrimidine ( 5j ) showed high affinity for the
A3 receptors , with Ki values of 8.1 , 10.4 , and 12.1 nM , respectively , and were & gt;1000 - fold selective versus all other
adenosine receptor subtypes . -
pubmed
Spermidine stimulates
T cell protein - tyrosine phosphatase - mediated protection of intestinal epithelial barrier function . -
pubmed
A previous study demonstrated that the small molecule
spermidine is a selective activator of
TCPTP in vitro . -
pubmed
On a functional level ,
spermidine protected barrier function in the setting of
inflammation , restricting the decrease in transepithelial electrical resistance and the increase in epithelial permeability induced by
IFN - gamma in coincubation experiments . -
pubmed
These data implicate
spermidine as a potential therapeutic agent to treat conditions associated with elevated
IFN - gamma signaling and a faulty mucosal barrier . -
pubmed
Heme is a suggested limiting factor in
peroxidase production by Aspergillus spp . -
pubmed
In this study , the role of genes coding for
coproporphyrinogen III oxidase (
hemF ) and
ferrochelatase (
hemH ) was analyzed by means of deletion and overexpression to obtain more insight in fungal
heme biosynthesis and regulation . -
pubmed
Nevertheless , both gene deletion mutants showed an extremely impaired growth even with
hemin supplementation which could be slightly improved by media modifications and the use of
hemoglobin as
heme source . -
pubmed
Overexpression of
hemH , but not
hemF or the
aminolevulinic acid synthase encoding
hemA , modestly increased the cellular
heme content , which was apparently insufficient to increase activity of endogenous
peroxidase and
cytochrome P450 enzyme activities . -
pubmed
Second trimester
thyroid - stimulating hormone , total and free
thyroxine reference intervals for the Beckman Coulter Access 2 platform . -
pubmed
The association between the
ERCC1 / 2 polymorphisms and the clinical outcomes of the
platinum - based chemotherapy in
non - small cell lung cancer (
NSCLC ): a systematic review and meta - analysis . -
pubmed
OBJECTIVE : To prospectively investigate the association between serum
uric acid ( SUA ) level and incidence of
impaired fasting glucose (
IFG ) in adult Chinese . -
pubmed
Indeed ,
omalizumab has been reported to be effective in a number of
IgE - mediated and non -
IgE - mediated disorders . -
pubmed
Concerning non -
IgE - mediated disorders ,
omalizumab has been reported to be effective in
nasal polyposis ,
autoimmune urticaria ,
chronic idiopathic urticaria ,
physical urticaria ,
idiopathic angioedema , and
mastocytosis . -
pubmed
Then P.e LPS was treated with
Ca ( OH ) 2 for 30 mins or 60 mins at 37 degrees centigrade in vitro and the activity of P.e LPS was evaluated by Chromogenic End - point Tachypleus Amebocyte Lysate ( CE
TAL ) test . -
pubmed
P.e LPS treated with 10 % and 15 %
Ca ( OH ) 2 both presented the best results by CE
TAL and significant difference compared with P.e LPS control group . -
pubmed
Post radiopacity was measured in millimetres of
aluminium ( mmAl ) with reference to an
aluminium step wedge . -
pubmed
Significant inverse correlation between
high - density lipoprotein cholesterol and
apoA with angiographic score was identified in women ( r = -0.23 , p = 0.028 and r = -0.26 , p = 0.025 ) , but not in men . -
pubmed
One -
pot preparation of
glutathione -
silica hybrid monolith for mixed - mode capillary liquid chromatography based on "
thiol - ene " click chemistry . -
pubmed
A novel
glutathione (
GSH)-
silica hybrid monolithic column synthesized via a combination of
thiol - ene click reaction and one -
pot process was described , where
thiol - end
GSH organic monomer and
2,2 - azobisisobutyronitrile (
AIBN ) were mixed with hydrolyzed
tetramethyloxysilane (
TMOS ) and
gamma - methacryloxypropyltrimethoxysilane (
gamma - MAPS ) and then introduced into a fused -
silica capillary for simultaneous polycondensation and "
thiol - ene " click reaction to form the
GSH -
silica hybrid monolith . -
pubmed
In addition , the
GSH -
silica hybrid monolith was applied to the separation of
nucleotides , peptides and
protein tryptic digests , respectively . -
pubmed
Effects of the
hydroxyl group on
phenyl based ligand /
ERRgamma protein binding . -
pubmed
We used the implicit Molecular Mechanics / Poisson - Boltzmann Surface Area ( MM / PBSA ) method to estimate the free energies of binding for the
phenyl based compound /
ERRgamma systems . -
pubmed
Free binding energies of
BPA - D to the
ERRgamma were found to be considerably less than those of
BPA - A and
BPA - C to the
ERRgamma . -
pubmed
A number of
molecules have been identified as regulators of this process , including
p63 ( also known as
tumor protein 63 ) and
Notch1 . -
pubmed
Mutation of
EpCAM leads to intestinal barrier and
ion transport dysfunction . -
pubmed
Cells with
EpCAM deficiency exhibited decreased electrical resistance , increased permeability , and decreased
ion transport . -
pubmed
Cells with
EpCAM deficiency demonstrated barrier and
ion transport dysfunction . -
pubmed
There is increasing evidence that local
thyroid hormone (
TH ) availability changes profoundly in inflammatory conditions due to altered expression of
deiodinases that metabolize
TH . -
pubmed
Wide - angle X - ray scattering in conjunction with
pole figure technique was used to study the texture of
poly ( vinylidene fluoride ) (
PVDF ) alpha and gamma phase crystals in nanolayered
polysulfone /
poly ( vinylidene fluoride ) films (
PSF /
PVDF ) produced by layer - multiplying coextrusion . -
pubmed
A large fraction of those crystals was oriented with macromolecular chains perpendicular to the
PSF /
PVDF interface as evidenced from the ( 021 )
pole figures . -
pubmed
The gamma crystals orientation was probed with the ( 004 )
pole figures showing the c - axis of
PVDF gamma crystals perpendicular to the
PSF /
PVDF interface . -
pubmed
Herein , atom - based 3D - QSAR analysis was performed using receptor - guided alignment of 46
flavonoid inhibitors of
aldose reductase (
ALR2 ) enzyme . -
pubmed
6 products derived from
sulfonamide S -- N bond cleavage ,
aniline moiety oxidation and
Smiles - type rearrangement were identified , and transformation pathways of
SMZ oxidation were proposed . -
pubmed
Multinomial conditional logistic regressions showed that elderly individuals with
T2DM and
PD were more likely to have HbA1c and lipid (
HbA1c & lt ; 8 % ;
LDL - C & lt;100 mg / dl ;
HDL - C & gt;= 50 mg / dl ;
triglyceride & lt;150 mg / dl ; and total
cholesterol & lt;200 mg / dl ) control . -
pubmed
The analyses resulted in several important findings : 1 ) Main components are additive and additive - by - additive effects for
GCA and dominance - related effects for
SCA and
MPH , and additive - by - dominant effect for
MPH was partly identified as additive effect ; 2 ) the ranking of factors affecting heterosis was dominance & gt ; dominance - by - dominance & gt ; over - dominance & gt ; complete dominance ; and 3 ) increasing the proportion of F1 hybrids in the population could significantly increase the power to detect dominance - related effects , and slightly reduce the power to detect additive and additive - by - additive effects . -
pubmed
Here we studied the
calcium channels that contribute to fast - gated BK channel activation and increased excitability of
beta4 knockout neurons . -
pubmed
In addition to chemical analyses in samples of effluents , surface waters , sediments , and fish , ( 1 )
dioxin - like potentials were measured in paired samples of effluents , surface waters , and sediments by use of an in vitro biotest ( reporter gene assay ) and ( 2 )
dioxin - like effects were investigated in exposed fish by use of in vivo activity of the mixed - function ,
monooxygenase enzyme ,
ethoxyresorufin O - deethylase (
EROD ) in liver . -
pubmed
All advanced technologies studied , based on degradation or adsorption , significantly reduced
dioxin - like potentials in samples and resulted in lesser
EROD activity in livers of fish . -
pubmed
We also evaluated the effect of
vemurafenib in four V600
BRAF melanoma cell lines with / without
MC1R variants . -
pubmed
Data in vitro showed a significant different phosphorylation of
Erk1 / 2 and
p38 MAPK during treatment , associated with a greater increase in
vemurafenib IC50 in
MC1R variant cell lines . -
pubmed
Therefore , the elevated catalytic activity toward the EOR at
PtSn - based electrodes is mainly associated with the improved
CO tolerance and the incomplete oxidation of
ethanol to form
acetic acid and
acetaldehyde species , causing the formation of a higher amount of both
C2 products with the rise of
Sn loading . -
pubmed
While it induced a significant increase of serum
insulin ( 3.41 + - 0.08 and 3.28 + - 0.08 vs. 2.39 + - 0.27 ) ,
HDL -
cholesterol , total antioxidant capacity levels , beta cell function percent , and pancreatic reduced
glutathione (
GSH ) content ( p & lt ; 0.05 ) and improved histopathological changes of the pancreas . -
pubmed
We experimentally present the radiative heat induced thermo - optic action detectable by
SPR shift through imaging of a thermal source onto a bulk metal grating substrate with IR - absorptive
silicon nitride coating . -
pubmed
Rhein exhibits antitumorigenic effects by interfering with the interaction between
prolyl isomerase Pin1 and
c - Jun. The
Pin1 protein ( or
peptidyl - prolyl cis / trans isomerase ) specifically catalyzes the cis / trans isomerization of phosphorylated
serine /
threonine -
proline (
Ser /
Thr -
Pro ) bonds and plays an important role in many cellular events through the effects of conformational change in the function of
c - Jun , its biological substrate . -
pubmed
Pin1 phosphorylation has been regarded as a marker of
Pin1 isomerase activity , and the phosphorylation of
Ser /
Thr -
Pro on protein substrates is prerequisite for its binding activity with
Pin1 and subsequent isomerization . -
pubmed
Since phosphorylation of proteins on
Ser /
Thr - Pro is a key regulatory mechanism in the control of cell proliferation and transformation ,
Pin1 has become an attractive molecule in
cancer research . -
pubmed
Here , we demonstrated the antitumorigenic effect of
rhein using cell proliferation assay , anchorage - independent cell transformation , pull - down assay ,
luciferase promoter activity , fluorescence - activated cell sorting and western blot analysis . -
pubmed
The
rhein /
Pin1 association was found to play a regulatory role in cell proliferation and
neoplastic cell transformation and the binding of phosphorylated
c - Jun (
Ser73 ) with
Pin1 was markedly decreased and inhibited
activator protein 1 or
NF - kappaB reporter activity by
rhein . -
pubmed
Overall , our findings and the accompanying biochemical data demonstrated the antitumorigenic effect of
rhein through its interference in
Pin1 /
c - Jun interaction and suggest the possible use of
rhein in suppressing the
tumor - promoting effects of
Pin1 . -
pubmed
Expert opinion : The phase
II open label trial suggested that
edaravone is safe and effective in
ALS , markedly reducing
3 - nitrotyrosine levels in the cerebrospinal fluid . -
pubmed
Volume overload and activation of the
renin -
angiotensin -
aldosterone system play a major role in the pathophysiology of
hypertension . -
pubmed
The hydrochemistries and
carbon isotopic signatures of dissolved inorganic
carbon from groundwater were measured during the rainy season ( July ; 41 samples ) and
post - rainy season ( October ; 26 samples ) . -
pubmed
The mean contribution of atmospheric / pedospheric
CO2 to the total aquatic
HCO3 - decreased by 15.67 % ( rainy season ) and 14.17 % (
post - rainy season ) due to the contributions made by these acids . -
pubmed
BACKGROUND : The use of
GnRH analogue medication is essential in reproductive medicine to avoid premature ovulation by pituitary suppression for the duration of ovarian stimulation by
gonadotrophins . -
pubmed
Changing the chirality of one of the residues has consequences on the
CH pi interaction , which is of CalphaH pi nature for
LD , while LL shows a
CbetaH pi interaction . -
pubmed
Antitumor effects of
metformin are a result of inhibiting
nuclear factor kappa B nuclear translocation in
esophageal squamous cell carcinoma . -
pubmed
Metformin exerts antitumor effects in various respects , such as inhibiting
inflammation ,
tumor growth and epithelial - mesenchymal transition (
EMT ) . -
pubmed
We found that localization of
NF - kappaB in the nucleus was reduced after
metformin treatment . -
pubmed
This suggests that
metformin inhibited the activation of
NF - kappaB. -
pubmed
Metformin inhibited cell motility and induced
E - cadherin expression . -
pubmed
In conclusion ,
metformin showed multiple antitumor effects such as growth suppression , invasion inhibition , and control of
EMT by inhibiting
NF - kappaB localization on
ESCC . -
pubmed
The as - synthesized
Ag NPs were characterized by using UV - Visible spectroscopy , X - ray diffraction and transmission electron microscopy (
TEM ) . -
pubmed
The
TEM micrographs have revealed that spherical shaped
Ag NPs with polydisperse in size were obtained . -
pubmed
Brain
glucose consumption abnormalities in
neuro - Behcet ' s disease : a preliminary
18F - FDG PET /
CT study . -
pubmed
Through an integrated analysis of
RCC metabolic pathways , we showed that
methylthioadenosine phosphorylase (
MTAP ) and its substrate
methylthioadenosine (
MTA ) are dysregulated in aggressive
RCC . -
pubmed
Interestingly , we found a decrease in the protein methylation level with a concomitant increase in
tyrosine phosphorylation after
MTAP knockout . -
pubmed
A
phospho - kinase array screen identified the
type 1 insulin - like growth factor-1 receptor (
IGF1R ) as the candidate with the highest upregulation in
tyrosine phosphorylation in response to
MTAP loss . -
pubmed
IGF1R suppression by a selective inhibitor of
IGF1R ,
linsitinib , impaired the cell migration and invasion capability of
MTAP - deleted cells . -
pubmed
Surprisingly , an increase in
linsitinib - mediated
cytotoxicity occurred in
RCC cells with
MTAP deficiency . -
pubmed
Polyethylene Glycol Acts as a Mechanistic Stabilizer of
L - asparaginase : A Computational Probing . -
pubmed
BACKGROUND :
L - asparaginase (
L - ASN ) is an anti -
cancer enzyme therapeutic drug that exerts
cytotoxicity via inhibition of protein synthesis through depletion of
L - asparagine in the
tumor microenvironment . -
pubmed
OBJECTIVE : In this study , we attempted the modification of recombinant
L - asparaginase with
PEG and an integrated computational strategy to probe the PEGylation in the protein to understand the biological stability / activity imparted by
PEG . -
pubmed
The molecular dynamics analysis revealed intricate interactions required in the coupling of
PEG to
L - asparaginase to bestow stronger binding affinity of
L - asparagine moiety towards
L - asparaginase . -
pubmed
PEG mechanistically stabilized
L - asparaginase through inducing pocket modification at the receptor to adapt to the cavity . -
pubmed
Thiamethoxam /
clothianidin significantly up - regulated the expression of
cyp17 and
cyp19 genes in the testis , which ultimately led to a significant decrease in
testosterone levels and a significant increase in the
17 - estradiol concentrations in plasma . -
pubmed
Inspired by these views , we synthesized
VO xQDs herein via a one -
pot top - down
ethanol - thermal method using bulk
VO2 as the precursor . -
pubmed
The
VO xQDs showed not only
oxidase- and
peroxidase - like activities in
ethanol as the main background solution (
ethanol -
BGS ) , but also exhibited additional
superoxide dismutase mimetic activity in
phosphate buffer solution . -
pubmed
The applicability of the
VO xQDs based 3D -
CS was further proved via the facile and accurate
H2O2 assays in three different practical samples . -
pubmed
RTT cells showed increased levels of nuclear
p65 and
ASC proteins , pro - IL-1beta mRNA , and
NLRP3 /
ASC interaction in basal condition , without any further response upon the LPS +
ATP stimuli . -
pubmed
Furthermore , the imaging of cellulose lattices in a C -
NCF /
TiO2 nanohybrid system is accomplished in the presence of
Ag+ ions in a medium revealing the mode of association of C - NCFs in the system , which validates the feasibility of the presented strategy . -
pubmed
The content of
malondialdehyde (
MDA ) in leaf exposed to
Cd was higher , and the
POD and
CAT activity exhibited a positive response to the low level of
Cd addition ( 5 mg L-1 ) . -
pubmed
Assisted by ab initio calculations , confirming that the agglomeration of
oxygen vacancies significantly change the local dielectric constants of the material , the results provide direct evidence that reduced dislocations can be classified as
alien matter embedded in the
SrTiO3 matrix . -
pubmed
Experiments with endothelial cells further confirm that a mix of
flavanol - derived colonic metabolites effectively down - regulate the levels of
p - p65 - NF - kappaB and the
cell adhesion molecules ICAM-1 and
VCAM-1 , preventing thus the increase of monocyte - endothelial adhesion induced by
TNF - alpha . -
pubmed
A total of 9324 unigenes were identified as differentially expressed genes (
DEGs ) , of which 5380 and 3944 genes were up- and down - regulated in the group treated with
bile acids , respectively . -
pubmed
Among them , '
AMPK signaling pathway ' which is critical in balancing cellular energy , was significantly enriched after
bile acids activation . -
pubmed
In contrast to aging kidneys ,
adenine - induced
nephropathy showed prominent expression of the genes related to
cytokines , T cell activation , and
fibrosis and decreased expression of the genes implicated in transporter activity and metabolism . -
pubmed
Recent NHP studies have found that ( 1 ) mixed mu opioid and
nociceptin /
orphanin FQ peptide receptor partial agonists appear to be safe , non - addictive analgesics and ( 2 )
mu opioid receptor- and mixed opioid receptor subtype - based medications remain the only two classes of drugs that are effective in alleviating opioid - induced adverse effects . -
pubmed
The majority of studies evaluating parathyroidectomy ( PTX ) for
SHPT use the criteria of
parathyroid hormone level (
PTH ) > 800 pg / ml with
hypercalcemia and/or
hyperphosphatemia , which may be accompanied by symptoms such as
bone pain and
pruritis that can improve after surgery . -
pubmed
,
99m - technetium - sestamibi scintigraphy with computed tomography [ SPECT /
CT ] ,
CT , or ultrasound ) is highly variable in
SHPT , SPECT /
CT appears to be the most sensitive . -
pubmed
Dermatan sulphate was correlated with soluble
syndecan-1 , and inversely correlated with blood pressure and activated partial
thromboplastin time . -
pubmed
The results show that an interplay between
syndecan-1 and
dermatan sulphate contributes to
sFlt1 induced blood pressure elevation in pre -
eclampsia . -
pubmed
The influence of
insulin upon the metabolism of
glucose by the brain . -
pubmed
The increased content of
stearic acid increased the density of the lipoproteins so that a larger proportion of the lipoproteins were in the
LDL and a smaller proportion were in the
VLDL than in lipoproteins from normal plasma and eggs . -
pubmed
The peptides studied had the general formula A
Leu Val - His - B. Hydrolysis at 37 degrees C and pH 2.07 occurred at the
amino side of the
leucine residue for all the
enzymes and all the peptides . -
pubmed
The preovulatory rise in the
oestradiol-17beta secretion rate , the
LH surge and the display of oestrus all occurred earlier , with respect to the
PGF-2alpha infusion , in November than in February , suggesting a greater stimulation of folliculogenesis , and therefore a greater availability of maturing follicles , in November than in February . -
pubmed
We raised high - titre antisera to two
LSD - bovine serum albumin conjugates , one linked via the
indole nitrogen , the other via the
amide side - chain . -
pubmed
The
antisera were specific for different parts of the LSD molecule , as demonstrated by cross - reactivity studies with
LSD , its metabolites ,
ergot alkoloids , and closely related compounds . -
pubmed
The
antisera were used to develop a double - antibody radioimmunoassay with a detection limit of about 0.4 mug of
LSD per liter of unextracted urine or serum . -
pubmed
From measurements of total ventilation ( ml min-1 kg-1 ) and of
halothane concentrations in inspired ( F1 ) end - tidal (
FE ' ) and ' mixed - spill ' ( FS ) gases , the following parameters were calculated for 5 - min intervals from 20 to 40 min after induction ; the rate of uptake of
halothane per percent inspired concentration ( Vha1 ml min-1%-1 ) and the degree of equilibrium achieved with the inspired concentration , calculated as FE'/F1 expressed as a percentage . -
pubmed
We engineered four expression vectors in which a synthetic oligomer encoding
gp120 residues 306 to 328 (
amino acids YNKRKRIHIGP GRAFYTTKNIIG ) from the V3 loop of the
MN viral isolate was inserted at various positions within the
endogenous HIV-1LAI env gene . -
pubmed
Thus ,
calcium - calmodulin dependent cellular processes are involved in both induction of
IFN and its action . -
pubmed
The several patterns response to
TFP and
W-7 may reflect different ligand - binding sites on
calmodulin . -
pubmed
The level of
DNA polymerase alpha activity was found to gradually decrease by 60 % ( 2.3 to 0.8 nmol of
[ 3H ] dCMP incorporated / mg protein / h ) between 9- and 19 - day - old
ECB . -
pubmed
Analysis of
DNA polymerase alpha from 11- and 19 - day - old ECB by a 10 to 30 %
glycerol density gradient revealed a high molecular weight peak sedimenting near
catalase ( 11.3 S ) with activity at the 11th day being approximately 3 - fold greater than activity at the 19th day . -
pubmed
The data are consistent with the view that brain
anoxia initially increases
FFA by receptor - mediated
polyphosphoinositide breakdown and that the
alpha-1 adrenergic receptor is one of the receptors involved . -
pubmed
The data also indicate that the fight and flight reaction is dissociated from the events that lead to
FFA release , and may involve the stimulation of
glutaminergic NMDA receptors . -
pubmed
To investigate the mechanism involved in the
PDH responsiveness to
insulin in circulating lymphocytes and the role of
Ca2 + and
Mg2 + in this process , the
PDH activity was assayed in lymphocytes combined with
insulin and/or a number of substances whose mechanism of action is partially known . -
pubmed
Relation of
amiodarone hepatic and
pulmonary toxicity to serum drug concentrations and
superoxide dismutase activity . -
pubmed
Specific effects of
GM3 on
tyrosine phosphorylation of the
epidermal growth factor receptor . -
pubmed
Neither
GM3 nor
GM1 had any affect on the binding of 125I -
EGF to its
cell surface receptor . -
pubmed
( i )
EGF - dependent
tyrosine phosphorylation of the
EGF receptor and its inhibition by
GM3 were also demonstrated on isolated
EGF receptor after adsorption on the
anti - receptor antibody - Sepharose complex , and the receptor phosphorylation was enhanced on addition of
phosphatidylethanolamine . -
pubmed
( ii )
Phosphoamino acid analysis of the
EGF receptor indicated that the reduction of phosphorylation induced by
GM3 was entirely in the
phosphotyrosine and not in the
phosphoserine nor
phosphothreonine content . -
pubmed
These results suggest that membrane lipids , especially
GM3 , can modulate
EGF receptor phosphorylation in vitro as well as in situ . -
pubmed
The occupational groups studied were not standardized for factors known to affect
bilirubin ,
GGT ,
cholesterol and HDL . -
pubmed
Quaternary ammonium sulfanilamide : a membrane - impermeant
carbonic anhydrase inhibitor . -
pubmed
A novel
carbonic anhydrase (
CA ) inhibitor ,
quaternary ammonium sulfanilamide (
QAS ) , was tested for potency as a
CA inhibitor and for its ability to be excluded from permeating biological membranes . -
pubmed
Mechanism of
azide binding to
chloroperoxidase and
horseradish peroxidase : use of an
iodine laser temperature - jump apparatus . -
pubmed
The kinetics of
azide binding to
chloroperoxidase have been studied at eight pH values ranging from 3.0 to 6.6 at 9.5 + /- 0.2 degrees C and ionic strength of 0.4 M in
H2O. -
pubmed
In addition , results were obtained on
azide binding to
horseradish peroxidase at pD 4.36 and pH 4.56 . -
pubmed
The H / D solvent isotope effects indicate proton transfer is partially rate controlling and is more important in the dissociation of
azide from the
enzyme - ligand complex . -
pubmed
A mechanism is proposed in which
hydrazoic acid binds to
chloroperoxidase in a concerted process in which its proton is transferred to a distal basic group . -
pubmed
The binding rate constant data , kon , can be fit to the equation kon = k3/(1 + KA/[H+ ] ) , where k3 = 7.6 X 10(7 ) M-1 S-1 and KA , the dissociation constant of
hydrazoic acid , is 2.5 X 10(-5 ) M. The same mechanism probably is valid for the ligand binding to
horseradish peroxidase . -
pubmed
Following acid hydrolysis and
diethylether extraction ,
phenol is separated from other urinary components within 3 minutes using the proposed capillary
GC method . -
pubmed
General abdominal symptoms , haematological status , basal ( BAO ) and maximal ( MAO )
hydrochloric acid secretion were studied before ( I ) and 1 year (
II ) and 14 years ( III ) after the operation . -
pubmed
It was therefore concluded that
histamine blocks not only the specific
ACh binding site but also interacts with the
ACh - channel site . -
pubmed
The effects of cations on the inhibition of
sodium and potassium activated adenosinetriphosphatase by
beryllium . -
pubmed
The characteristics of
alkaline phosphatase of the cells treated or untreated with
cycloheximide or
actinomycin D were similar to each other ; they were heat labile and the
enzyme reaction was strongly inhibited by
L - homoarginine , but weakly by
L - phenylalanine . -
pubmed
The increase in
alkaline phosphatase activity with
cycloheximide has been termed a ' superactivation ' of
alkaline phosphatase . -
pubmed
Epithelial endocrine cells containing
5 - hydroxytryptamine ,
substance P ,
somatostatin , enteroglucagon and
vasoactive intestinal polypeptide - immunoreactivity were localized by immunocytochemistry in the mucosa of normal appendices , ileum and proximal colon , and in
neurogenic appendicopathy . -
pubmed
To examine the role in neurite growth of
actin - mediated tensions within growth cones , we cultured chick embryo dorsal root
ganglion cells on various substrata in the presence of
cytochalasin B. -
pubmed
On highly adhesive substrata , nerve cells were observed to extend numerous ( though peculiarly oriented ) neurites in the presence of
cytochalasin , despite their lack of both filopodia and lamellipodia or the orderly
actin networks characteristic of typical growth cones . -
pubmed
In
primary biliary cirrhosis the hepatic
copper content was correlated with
bilirubin and
alkaline phosphatases but not with
ceruloplasmin ,
coagulation factors , or
aminotransferases . -
pubmed
We conclude that the hepatic
copper content and
serum ceruloplasmin were remarkably constant during 3 years of observation in various
liver disorders . -
pubmed
Retinoids modulate cellular proliferation and mediate gene function through a series of
nuclear receptors . -
pubmed
In contrast to the
RAR beta - expressing cells , colony formation by the
RAR alpha - expressing cells was similar to the mock - transfected controls and the addition of 1 microM
RA to the
RAR alpha - transfected cells inhibited colony formation . -
pubmed
In a preceding study , we established the tolerance and pharmacokinetic behavior of
5 - fluoro-2 '- deoxyuridine (
FdUrd ) given by the intraperitoneal (
IP ) route . -
pubmed
Pharmacologic determinations of
IP FdUrd and
5 - Fluorouracil (
FUra ) derived from it and LV were obtained by HPLC methods on 11 occasions . -
pubmed
We conclude that
IP FdUrd 3 g and
LV in doses of up to 640 mg x 3 days are well tolerated . -
pubmed
Since
FdUrd is more potent , has an even greater hepatic clearance and shows greater potential for modulation with LV than
FUra , it may be the preferred
fluoropyrimidine for subsequent studies via the
IP route in the treatment of
carcinomas with prominent peritoneal spread . -
pubmed
Analysis of the resulting
drug - resistant populations revealed that cells producing
Asp95 DHFR dominated with an overall frequency of 3:1 , and therefore , under these growth conditions , display a selective advantage over those producing Asn95
DHFR . -
pubmed
Refolding of the soluble recombinant binding - active extracellular domain of the
murine erythropoietin receptor (
sEPO - R ) was achieved with greater than 90 % recovery either from
urea /
NaCI ( 1.5/0.5 M ) or
guanidine HCI ( 1 M ) . -
pubmed
Methyl groups are sequentially eliminated as
HCHO by incorporation of
O2 catalyzed by
DMS monooxygenase and
methanethiol oxidase . -
pubmed
H2O2 formed during
CH3SH oxidation is destroyed by
catalase . -
pubmed
The inhibition was completely abolished by
Sch23390 , a selective
D1 antagonist ( 0.5 - 2 mg / kg ) , but partially reversed by
SPD ( 1 - 16 mg / kg ) . -
pubmed
Treatment of synovial cells with
N - acetyl - L - cysteine (
NAC ) , an antioxidant agent , inhibited
TNFalpha - induced
NF - kappaB activation and transcription . -
pubmed
Moreover ,
NAC also inhibited synovial cell proliferation induced by
TNFalpha . -
pubmed
In the
troglitazone group , FPG and
HbA(1c ) decreased significantly after the treatment ( before vs after , FPG : 10.8 + /- 2.0 mmol I(-1 ) vs 9.2 + /- 2.5 mmol I(-1 ) , p< ; 0.001 ;
HbA(1c ): 9.2 + /- 1.4 % vs 8.5 + /- 1.5 % , p< ; 0.001 ) . -
pubmed
Serum total
cholesterol and
HDL - cholesterol did not change in either group , however , serum
triglyceride significantly decreased in the
troglitazone group . -
pubmed
In order to evaluate the relationship between primary colorectal
adenocarcinomas and their
metastases with regard to
AgNOR proteins expression , we have performed a morphometric analysis of AgNOR
silver precipitates ( AgNORs ) in advanced colorectal
carcinomas ( stage III -
IV ) as well as in corresponding lymph node and distant
metastases . -
pubmed
Therein ,
PGE2 and
IL-1 production was noted to coincide with the onset of
fever and the
coagulation disorders typical of
CSF . -
pubmed
Red blood cells have been labeled with an anti -
renin pharmacophore using the activated labeling agent
Boc - Phe - His - ACHPA - Ile-6 - NH ( CH2 ) 5CO - NHS ( 4 ) and the corresponding
sulfo - NHS - ester ( 5 ) . -
pubmed
Inhibitory activity of labeled red cells is clearly associated with the cells themselves , and does not require prior release of an inhibitory entity :
renin inhibition increases as a function of the concentration of
NHS - ester used to label cells suspended in buffer , and with cell density ; on the other hand , the separated supernatant portions of the medium make only minor contributions to the observed inhibitory activities . -
pubmed
The aims of this study were : ( 1 ) to analyze the effects of oral irrigation ( Water Pik Oral Irrigator ) on the clinical signs of adult
periodontitis (
AP ) and on the levels of
interleukin-1 beta (
IL - beta ) ,
prostaglandin - E2 (
PGE2 ) ,
interleukin-10 (
IL-10 ) and
interferon - gamma (
IFN - gamma ) in GCF , and ( 2 ) to analyze the influence of the
periodontitis - related
IL-1 genotype (
IL-1GT ) on these variables . -
pubmed
RESULTS : Oral irrigation plus
ROH resulted in a significant reduction in
PPD ,
BOP , GI and
PI , as well as
IL - beta levels by 7 days and
PGE2 levels by 14 days , relative to
ROH or no oral hygiene . -
pubmed
ROH reduced GI ,
BOP and
PI , and
PGE2 levels by 14 days , but had no effect on
IL-1 beta or
IL-10 levels relative to no oral hygiene . -
pubmed
However , limitations of this isomerization are as follows : ( i ) only
N -( p - methoxyphenyl )- beta - lactams can be used , and ( ii ) transformation is less compatible with heteroatomic substituents bonded to the
C3 position of the
2 - azetidinone ring . -
pubmed
Acanthoxin B and C showed lower enzymatic activities than
acanthoxin A ( 4.0 , 13.7 and 23.9 micromol of phospholipid hydrolyzed / min / mg
protein , respectively ) . -
pubmed
Other pesticides also dissolved in
DMSO --
mecoprop ,
simazine or
MCPA ( each up to 1 microM)--or dissolved in
phosphate - buffered saline --
diuron ( up to 1 microM ) ,
isoproturon ( up to 3 microM ) ,
metoxuron ( up to 8 microM ) or
metamitron ( up to 80 microM)--showed no concentration - related effects on
cytokine production . -
pubmed
There was , however , an inhibition of
IFN - gamma and
TNF - alpha production by
simazine ,
metoxuron and
mecoprop and of all three
cytokines tested by
diuron . -
pubmed
MCPA ( 0.01 and 0.1 microM ) stimulated the production of
TNF - alpha . -
pubmed
The binding of
methadone to plasma
proteins was enantioselective with the active ( -)-(R ) enantiomer having the highest free fraction ( mean + /- SD : 21.2+/-7.6 % vs. 13.3+/-6.2 % for
( + ) -( S )- methadone , n = 8) . -
pubmed
Recent clinical studies have documented the efficacy of
trastuzumab ( Herceptin ; Genentech , Inc , South San Francisco , CA ) as a new biologically targeted therapy for
erbB-2 receptor - positive forms of
breast cancer . -
pubmed
This mini - review highlights these recent findings and suggests the possibility that the loss of
erbB-2 receptor - dependent myocyte survival pathways may create a susceptibility for the onset of
heart failure in response to the
cardiotoxicity of
anthracycline treatment . -
pubmed
Myocardial protein levels of
CK isoforms ( Western blot ) ,
HEP levels , and
CK kinetics (
( 31 ) P MR spectroscopy ) were measured under basal conditions . -
pubmed
The rank order of activity with respect to
actin polymerization and release of toxic reactive
oxygen species was
eotaxin-3 =
eotaxin =
eotaxin-2 and
eotaxin =
eotaxin-2 & gt ;
eotaxin-3 =
monocyte chemotactic protein-3 =
monocyte chemotactic protein-4 =
RANTES , respectively . -
pubmed
Is a role of
phospholipase A ( 2 ) in
cholesterol gallstone formation phospholipid species - dependent ? -
pubmed
Phospholipase A ( 2 ) plays a role in
cholesterol gallstone formation by hydrolyzing
bile phospholipids into
lysolecithin and
free fatty acids . -
pubmed
Then the effect of digestion of
phosphatidylcholine by
phospholipase A ( 2 ) on bile metastability was assessed by spectrophotometry and video - enhanced differential contrast microscopy . -
pubmed
Addition of
phospholipase A ( 2 ) caused the release of free
fatty acids in a time - dependent manner . -
pubmed
Therefore , the role of
phospholipase A ( 2 ) in
cholesterol gallstone formation depends on the phospholipid species present in bile , so that
phospholipid species selection during hepatic excretion is , in part , crucial to the
cholesterol stone formation . -
pubmed
Neuroprotection of striatal neurons against
kainate excitotoxicity by
neurotrophins and
GDNF family members . -
pubmed
Here , we analysed the protective effects of
brain - derived neurotrophic factor ,
neurotrophin-3 ,
glial cell line - derived neurotrophic factor and
neurturin against the
excitotoxic damage induced by
kainate in striatal neurons in vitro and in vivo . -
pubmed
Our results show that the decrease in the number of cultured striatal
calbindin - positive neurons induced by
kainate was prevented by treatment with any of these factors . -
pubmed
We found that the numbers of striatal projection neurons (
calbindin - positive ) and striatal interneurons (
parvalbumin- or
choline acetyltransferase - positive ) were differentially decreased after
kainate lesion . -
pubmed
Thymosin beta4 is the most abundant member ; it is expressed in most cell types and is regarded as the main intracellular
G - actin sequestering peptide . -
pubmed
Here we show that
thymosin beta4 can be cross - linked to proteins such as
fibrin and
collagen by
tissue transglutaminase . -
pubmed
Thymosin beta4 is not cross - linked to many other proteins and its cross - linking to
fibrin is competed by another family member , thymosin beta10 . -
pubmed
We suggest that
thymosin beta4 cross - linking is mediated by
factor XIIIa , a
transglutaminase that is coreleased from stimulated platelets . -
pubmed
The effect of different grades of hydroxyethyl cellulose (
HEC ) and
hydroxypropyl methllcellulose ( HPMC ) on the film - formation and taste - masking ability for
ibuprofen granules was evaluated . -
pubmed
Because
irinotecan (
CPT-11 ,
Camptosar ) is a
topoisomerase I inhibitor with a broad spectrum of antitumor clinical activity , we investigated its activity in relapsed or refractory
non - Hodgkin ' s lymphomas (
NHLs ) . -
pubmed
The purpose of this article is to outline and review the five major clinical trials of beta - blocker therapy in chronic
heart failure ; the US
Carvedilol heart failure Program ( USCP ) , the
Cardiac Insufficiency Bisoprolol Study
II ( CIBIS -
II ) , the
Metoprolol CR / XL Randomized Intervention Trial in
chronic Heart Failure ( MERIT -
HF ) , the Beta - blocker Evaluation of Survival Trial ( BEST ) and the
Carvedilol Prospective Randomized Cumulative Survival trial ( COPERNICUS ) , and to aid the reader in the selection of appropriate candidates for beta - blocker therapy . -
pubmed
Conformationally rigid
N - acyl-5 - alkyl - L - prolyl - pyrrolidines as
prolyl oligopeptidase inhibitors . -
pubmed
In the
N - acyl - L - prolyl - pyrrolidine type of
prolyl oligopeptidase inhibitors the
L - prolyl group was replaced by different
5 - alkyl - L - prolyl groups , resulting in a series of
N - acyl-5 - alkyl - L - prolyl - pyrrolidines . -
pubmed
Since
N - amides of
5 - alkyl - L - prolines are conformationally more rigid than those of
L - proline , the main objective was to make more rigid
prolyl oligopeptidase inhibitors . -
pubmed
Microsomal
cytochrome p450 and/or
p450 reductase appear to be involved in the reductive metabolism of
1,2 - benzisoxazole moiety under hypoxic as well as oxic conditions . -
pubmed
Detailed kinetic analysis showed
SULT1C1 was more prolific in that it was able to
sulfate dopamine ,
tyramine , and
apomorphine , which
SULT1B1 was not . -
pubmed
F - actin arrangement and the distribution of
laminin ,
fibronectin , several
integrins and phosphorylated
Focal Adhesion Kinase (
FAK ) were studied using
rhodamine -
phalloidin and immunofluorescence . -
pubmed
Inactivation of the toxin with
1,10 phenanthroline showed that the catalytic activity is important for the effect of
jararhagin on cell migration ,
FAK phosphorylation and for the recruitment of alphav , but not as much for the anti - adhesive effect . -
pubmed
Allergy - like reactions may occur following administration of iodinated
contrast media ( CM ) , mostly in at - risk patients ( patients with history of previous reaction , history of
allergy , co - treated with
interleukin-2 or beta - blockers , etc . ) -
pubmed
This periplasmic process requires at least 10 essential components for
apo - Cyt c chaperoning , thio - oxidoreduction , and the delivery of
heme and its covalent ligation . -
pubmed
Previously , we have shown that overproduction of the putative
heme ligation components
CcmF and
CcmH(
Rc ) ( also called
Ccl1 and
Ccl2 ) can partially bypass the function of
CcmI on minimal , but not on enriched , media . -
pubmed
These findings indicate that , in the absence of
CcmI , interactions between the
heme ligation and thioreduction pathways become restricted for sufficient
Cyt c production . -
pubmed
We therefore suggest that
CcmI , along with its apo -
Cyt chaperoning function , is also critical for the efficacy of
holo - Cyt c formation , possibly via its close interactions with other components performing the final
heme ligation steps during
Cyt c biogenesis . -
pubmed
Reduction of
alachlor - induced
olfactory mucosal neoplasms by the
matrix metalloproteinase inhibitor
Ro 28 - 2653 . -
pubmed
The present studies were designed to confirm this latter finding and to explore the potential role of
MMPs in
alachlor - induced
olfactory carcinogenesis . -
pubmed
The morphology of
alachlor - induced
olfactory tumors was similar whether or not
Ro 28 - 2653 had been given ; the
MMP inhibitor itself had no impact on olfactory mucosal histology . -
pubmed
The
proteins were digested by the action of
trypsin ,
pepsin or
chemotrypsin as revealed by gel filtration and
SDS - PAGE analysis . -
pubmed
Philanthotoxins are uncompetitive antagonists of
Ca2 + -permeable
AMPA receptors presumed to bind to the pore - forming region , but a detailed molecular mechanism for this interaction is missing . -
pubmed
Together these studies provide strong evidence for a molecular mechanism by which
polyamine toxins antagonize the
AMPA receptor ion channel and provide the basis for rational development of uncompetitive antagonists of
AMPA receptors . -
pubmed
N -( 5 - chloro-1,3 - benzodioxol-4 - yl )- 7 -[ 2 -( 4 - methylpiperazin-1 - yl ) ethoxy ]- 5 - ( tetrahydro-2H - pyran-4 - yloxy ) quinazolin-4 - amine , a novel , highly selective , orally available , dual - specific
c - Src /
Abl kinase inhibitor . -
pubmed
We report here a novel subseries of
C-5 - substituted anilinoquinazolines that display high affinity and specificity for the
tyrosine kinase domain of the
c - Src and
Abl enzymes . -
pubmed
N -( 5 - Chloro-1,3 - benzodioxol-4 - yl )- 7 -[ 2 -( 4 - methylpiperazin-1 - yl ) ethoxy ]- 5 -( tetrahydro-2H - pyran-4 - yloxy ) quinazolin-4 - amine (
AZD0530 ) inhibits
c - Src and
Abl enzymes at low nanomolar concentrations and is highly selective over a range of
kinases . -
pubmed
Oxidative metabolism of
polyunsaturated fatty acids through
cyclooxygenases or
lipoxygenases can generate various lipid
peroxides and bioactive lipids , and regulate cellular proliferation , apoptosis , differentiation and senescence . -
pubmed
The
Se compound also significantly ( p<0.01 ) reduced microsomal lipid peroxidation and induced
glutathione - S - transferase activity in liver and lung when measured after 8th and 22nd week . -
pubmed
These findings will further strengthen the view on
organoselenocyanate as an effective
cancer chemopreventive agent against lung
carcinogenesis when applied at the
post - initiation phase . -
pubmed
Although it is not widely recognized clinically , functional forms of
hypothalamic hypogonadism are more than an isolated disruption of
gonadotropin - releasing hormone (
GnRH ) drive and reproductive compromise . -
pubmed
Spectrin maintains the lateral order in
phosphatidylserine monolayers . -
pubmed
We investigate the effect of the skeletal protein
spectrin on the lateral order in
dipalmitoyl phosphatidylserine monolayers spread on aqueous surfaces using grazing incidence X - ray diffraction . -
pubmed
Without
spectrin , the condensed lipid monolayer exhibits two - dimensional hexagonal packing , characterized by monotonic decrease in the d - spacing and increase in the degree of order with increasing surface pressure between 17 and 36 mN / m. Addition of
spectrin to the aqueous subphase at high pressures preserves the monolayers structural parameters unchanged from 36 to 25 mN / m. These results demonstrate for the first time that
spectrin could participate in sustaining the two - dimensional order in lipid domains through a direct interaction with
phosphatidylserine species . -
pubmed
Fructose feeding increased the blood pressure and levels of
insulin ,
triglyceride and
fatty acid . -
pubmed
A giant solid
tumor in the left maxillary
sinus was detected on head
CT and MRI , and this
tumor was destroying the maxilla and extending into the orbit , pterygoid muscle and posterior paranasal
sinuses . -
pubmed
An experimental procedure was used based on selective quenching of both
1,3 - di ( 1 - pyrenyl ) propane (
Py-3 - Py ) and
1,6 - diphenyl-1,3,5 - hexatriene (
DPH ) by
trinitrophenyl groups , and radiationless energy transfer (
RET ) from the
tryptophans of membrane proteins to
Py-3 - Py . -
pubmed
In addition , the interaction of
KBA and
AKBA with the organic anion transporter
OATP1B3 and the multi drug resistant proteins
P - glycoprotein and
MRP2 was evaluated using partly fluorescent - based assays . -
pubmed
Neither
KBA nor
AKBA could be identified as substrates of
P - glycoprotein . -
pubmed
However , both
KBA and
AKBA modulated the activity of
OATP1B3 and
MRP2 , indicating that therapeutic relevant interactions with other anionic drugs may be expected . -
pubmed
When using
p - nitrophenyl - beta-1,4 - N , N '- diacetyl - chitobiose as substrate , the overall catalytic efficiency , k(cat)/K(m ) of the improved
chitinase was 2.7- and 2.3 - fold higher than the average k(cat)/K(m ) of wild types at pH 3.0 and 6.0 , respectively . -
pubmed
In adjusted analyses , older age was associated with less appropriate treatment ( odds ratio [ OR ] per 5 years , 0.91 ; P=.01 ) , and patients with
LDL cholesterol & lt;or=130 mg / dL ( OR , 18.8 ; P<.001 ) and the low - risk group ( OR , 27.5 ; P<.001 ) were more likely to be managed appropriately compared with patients with
LDL & gt;or=190 mg / dL and those at high risk . -
pubmed
In vitro release of
dexamethasone or
bFGF from chitosan /
hydroxyapatite scaffolds . -
pubmed
Chitosan scaffolds containing
dexamethasone (
Dex ) or
basic fibroblast growth factor (
bFGF ) were developed to create alternative drug - delivery systems for possible tissue - engineering applications such as periodontal bone regeneration . -
pubmed
Dex and
bFGF were incorporated into scaffolds by embedding method ( solvent sorption method ) . -
pubmed
The initial loading amounts were varied as 300 , 600 and 900 ng
Dex per dry scaffold ( average dry weight is 3 mg ) and 50 or 100 ng
bFGF per dry scaffold to a range of deliverable doses . -
pubmed
However ,
bFGF release from all scaffolds with both loading amounts ( 50 ng or 100 ng ) was completed in 10 or 20 h. In order to prolong the release period of
bFGF , composite scaffolds were fabricated in the presence of
hydroxyapatite ( HA ) beads with average particle size of 40 mum . -
pubmed
These results suggested that chitosan scaffolds can be suitable for
Dex release ; however , the presence of HA in the chitosan scaffold is necessary to achieve the desired release period for
bFGF . -
pubmed
During
deoxycorticosterone acetate - salt
hypertension ,
CO was maintained , but
TPR was elevated compared with the baseline period . -
pubmed
Acute
cholinesterase inhibition assays with
carbofuran were performed on culture days 5 , 10 and 17 . -
pubmed
In a 14 - day subchronic study with
fenitrothion ,
total protein levels in the cultures were significantly lower than control values at all doses tested ( 4 , 8 and 20 mug / ml medium ) while
cholinesterase activity was unaffected at all but the highest dose . -
pubmed
We suggest that at high concentrations ,
L - serine decreases synthesis of
UDP - N - acetylmuramate - L - alanine by the
murC - encoded
ligase , weakening the cell wall and producing misshapen cells and lysis . -
pubmed
The inhibition by high
L - serine is overcome in several ways : by a large concentration of
L - alanine , by overproducing
MurC together with a low concentration of
L - alanine , and by overproducing
FtsW , thus promoting septal assembly and also by overexpression of the
glycine cleavage operon . -
pubmed
They secrete a wide range of peptide hormones and biogenic amine
serotonin and express neuronal markers such as
chromogranins A , B , C (
CgA , B , C ) and
neuron specific enolase (
NSE ) together with
synaptophysin . -
pubmed
The propensity of
prostate cancer cells to undergo a transdifferentiation pathway towards NE phenotype is due to several microenvironmental conditions such as
androgen depletion ( induced by
LH - RH analogs or antagonists , antiandrogens ,
5 - alpha - reductase inhibitors ) , ionizing - radiation therapy , adrenergic factors , increase in
interleukin-6 signaling cascade . -
pubmed
Aberrantly activated
glutamic acid decarboxylase - independent pathway for production of
GABA (
gamma - amino - butyric acid ) appears to be a constant feature of invasive NE
tumors . -
pubmed
Intriguingly , intermittent
androgen deprivation therapy , by preventing NE differentiation , significantly reduces the risk of a rise in serum
CgA levels meanwhile delaying the time of
cancer progression due to hormone - independence . -
pubmed
Low amounts of
N - glucuronides were generated by
HLM , HIM , and HKM , as well as by
MLM and
CLM , but not by
RLM , according to the observed species specificity of this metabolic pathway . -
pubmed
We identified 6
molecules consistently present on
CD133 + cells :
CD9 ,
CD29 ,
CD49b ,
CD59 ,
CD151 , and
CD326 . -
pubmed
By contrast ,
CD24 ,
CD26 ,
CD54 ,
CD66c ,
CD81 ,
CD90 ,
CD99 ,
CD112 ,
CD164 ,
CD166 , and
CD200 showed a discontinuous behavior , which led us to identify cell type - specific surface
antigen mosaics . -
pubmed
RESULTS : Although the DP group had greater water ( p & lt ; 0.01 ) and feed ( p & lt ; 0.001 ) consumption , the DH group had greater
body weight ( p & lt ; 0.5 ) and adipose tissue gain ( p & lt ; 0.001 ) , favoring higher adiposity index ( p & lt ; 0.001 ) ,
glucose ( p & lt ; 0.01 ) , and area under the curve in the
insulin ( p & lt ; 0.001 ) and
glucose ( p & lt ; 0.01 ) tolerance tests . -
pubmed
OBJECTIVE : The use of
technetium-99 m methylene diphosphonate single - photon emission tomography / computed tomography (
99mTc - MDP SPECT /
CT ) of the spine and sacrum has increased over the past years ; however , there is little information about the optimal clinical role and true clinical impact of this modality . -
pubmed
METHODS : All
99mTc - MDP SPECT /
CT examinations of the spine and sacrum performed at our large multispecialty institution between 1 January 2008 and 12 April 2012 were identified . -
pubmed
RESULTS : Of the 212
99mTc - MDP SPECT /
CT examinations of the spine and sacrum identified , 191 ( 90 % ) were for
pain evaluation , 14 ( 7 % ) were to assess
infection , and seven ( 3 % ) were to evaluate a potential
malignancy . -
pubmed
CONCLUSION :
99mTc - MDP SPECT /
CT of the spine and sacrum is most frequently used for the diagnosis and management of
pain . -
pubmed
Although inflammatory nodules are more common with permanent fillers such as
silicone , inflammatory nodule development following administration of temporary fillers such as
hyaluronic acid and
collagen has also been reported . -
pubmed
Serum
25 ( OH ) D ,
IL-17 and
IL-23 levels were detected by ELISA . -
pubmed
While
25 ( OH ) D levels were not associated with radiographic bone erosions of
RA , they were significantly lower in those with
osteopenia and
osteoporosis than in those with normal
BMD ( P & lt ; 0.01 ) . -
pubmed
These data suggest that disruption of
chloride homeostasis in the
ER disrupts the protein - folding capacity of the
ER , leading to eventual neuron
death . -
pubmed
PURPOSE : To evaluate the efficacy of two
silicone hydrogel bandage lenses ( SiHy ) on epithelial healing following photorefractive keratectomy (
PRK ) with anterior segment optical coherence tomography (
AS - OCT ) . -
pubmed
The mRNA expression , protein levels and enzyme activities of key enzymes involved in
taurine biosynthesis ,
cysteine dioxygenase (
CDO ) and
cysteine sulfinate decarboxylase (
CSD ) , were analyzed . -
pubmed
RB and
PB showed lower MIC than AA and
TBO . -
pubmed
funiculosum and the results showed the increasing inhibition rate in the order of AA & lt ;
TBO & lt ;
PB & lt ; RB . -
pubmed
The efficacy of the solution containing an anionic detergent agent (
SDS ) was shown to be generally higher than that of IPA and , after the
SDS cleaning procedure , biosafety cabinets demonstrated acceptable contamination levels . -
pubmed
Here , we investigated the role of
IL-33 , a novel
interleukin ( IL )- 1 family member , and its receptor
ST2 in the analgesic effects of electroacupuncture ( EA ) on
formalin - induced inflammatory
pain . -
pubmed
These data suggest that EA alleviates
formalin - induced inflammatory
pain , at least partially , by inhibiting of spinal
IL-33 /
ST2 signaling and the downstream
ERK and
JNK pathways . -
pubmed
Reverse transcription -
polymerase chain reaction ( RT - PCR ) revealed that
TREM-1 mRNA was constitutively expressed at a low level in resting cells , and that its expression was upregulated by treatment with
vitamin D3 (
VitD3 ) , but not by LPS . -
pubmed
In addition , a
luciferase reporter assay revealed that the serum response element ( SRE ) was involved in
VitD3 - induced promoter activity , whereas the
activator protein-1 ( AP-1 ) sites participated in the
VitD3- and LPS - induced promoter activity . -
pubmed
Of note , the
CCAAT - enhancer - binding protein ( C /
EBP ) site contributed not only to basal , but also to
VitD3- and LPS - induced promoter activity . -
pubmed
Transfection with transcription factor oligodeoxynucleotide ( ODN ) decoys indicated that
transcription factors of the
C / EBP and AP-1 families are likely involved in the basal , as well as in the
VitD3- and LPS - induced
TREM-1 transcription . -
pubmed
Western blot analysis indicated that , of the members of the
C / EBP family ,
C / EBPalpha was constitutively expressed in resting cells ; its expression was enhanced by treatment with
VitD3 and was further increased by treatment with
VitD3 and
LPS . -
pubmed
Moreover , the expression of
c - Fos and
c - Jun ( members of the
AP-1 family ) was augmented by treatment with both
VitD3 and
LPS . -
pubmed
Our previous study showed that the downstream of
kinase 3 (
DOK3 ) is degraded during macrophage stimulation with
CpG. -
pubmed
In this study , we demonstrate that
CpG treatment leads to
ubiquitin - mediated degradation of
DOK3 via interaction with an
E3 ligase
TNFR - associated factor 6 (
TRAF6 ) . -
pubmed
We also identified the 27th
amino acid (
lysine ) of
DOK3 is responsible for
Ly48 polyubiquitination of
DOK3 . -
pubmed
Furthermore , reintroduction of
DOK3 ( K27R ) into
DOK3 - deficient macrophages abolishes
DOK3 degradation induced by
CpG and suppresses the production of
IL-6 and
TNFalpha . -
pubmed
More importantly , our study uncovers a novel role of an
E3 ligase
TRAF6 , namely ,
TRAF6 is also able to catalyse
Lys 48 polyubiquitylation of target protein except for
Lys 63 polyubiquitylation . -
pubmed
Anti - inflammatory Effects of
Poly - L - lysine in Intestinal Mucosal System Mediated by
Calcium - Sensing Receptor Activation . -
pubmed
Poly - L - lysine (
PL ) is a basic polypeptide identified for its role in the activation of
CaSR through allosteric binding . -
pubmed
As shown by the results from both in vitro and in vivo studies , the reduction of inflammatory
cytokine production caused by
PL was reversed by
NPS-2143 pretreatment . -
pubmed
A new type of
siloxane core modified
phthalide cardo chain based
polyimide (
PI ) was successfully prepared from
siloxane core
dianhydride and
ether linked
phenolphthalein diamine moiety . -
pubmed
This
PI was further modified with different weight percentages of multi - walled
carbon nanotubes ( MWCNTs ) to obtain MWCNT reinforced
PI nanocomposites . -
pubmed
Analogs of
farnesyl diphosphate alter
CaaX substrate specificity and reactions rates of
protein farnesyltransferase . -
pubmed
We characterized the reactivity of four
alkyne - containing analogs of
FPP with purified
protein farnesyltransferase and a small library of dansylated peptides using an in vitro continuous spectrofluorimetric assay . -
pubmed
The interaction of two
steroidal saponins ,
dioscin and
pseudo - protodioscin , from a botanical drug was studied for their equilibrium time and equilibrium amount by re - ligand fishing using
HSA functionalized magnetic nanoparticles . -
pubmed
Pigment composition of beads was determined by HPLC -
MS -
MS and degradation products were also analysed after storage ;
betalamic acid being the major one . -
pubmed
Metformin stimulates
IGFBP-2 gene expression through
PPARalpha in
diabetic states . -
pubmed
The anti - diabetic drug ,
metformin , exerts its action through
AMP - activated protein kinase (
AMPK ) , and
Sirtuin (
Sirt1 ) signaling . -
pubmed
In this study , we demonstrate that
metformin upregulates
Igfbp-2 expression through the
AMPK -
Sirt1 -
PPARalpha cascade pathway . -
pubmed
Notably , activation of
IGF-1 receptor (
IGF-1R)-dependent signaling by IGF-1 was inhibited by
metformin . -
pubmed
Taken together , these findings indicate that
IGFBP-2 might be a new target of
metformin action in
diabetes and the
metformin -
AMPK -
Sirt1 -
PPARalpha -
IGFBP-2 network may provide a novel pathway that could be applied to ameliorate
metabolic syndromes by controlling IGF-1 bioavailability . -
pubmed
GC -
MS results showed that SFWP had a backbone composed of ( 1 2)-linked
Glc , ( 1 2,6)-inkedGal and ( 1 3,6)-inked Man residues , which were terminated with ( 1 ) -inked
Xyl and ( 1 ) -inked
Ara at O-6 of ( 1 2,6)-inkedGal and ( 1 3,6)-inked
Man along the main chain , in the ratio of 2.0 : 1.02 : 1.09 : 1.10 : 0.98 . -
pubmed
2,3,7,8 - Tetrachlorodibenzo - p - dioxin (
TCDD ) is a persistent environmental contaminant and high - affinity ligand for the
aryl hydrocarbon receptor (
AhR ) . -
pubmed
Likewise ,
TCDD treatment increased mRNA expression of HSC activation and fibrogenesis genes , namely
alpha - smooth muscle actin ,
desmin ,
delta - like homolog-1 ,
TGF - beta1 , and
collagen type I. -
pubmed
However ,
TCDD treatment did not exacerbate
fibrosis , nor did it increase the
collagen content of the liver . -
pubmed
Instead ,
TCDD increased hepatic
collagenase activity and increased expression of
matrix metalloproteinase ( MMP )- 13 and the matrix regulatory proteins ,
TIMP-1 and
PAI-1 . -
pubmed
These results support the conclusion that
TCDD increases
CCl4 - induced
liver damage and exacerbates HSC activation , yet
collagen deposition and the development of
fibrosis may be limited by
TCDD - mediated changes in extracellular matrix remodeling . -
pubmed
Incorporation of
GP increased roughly 2 - to-3 - fold the
phenolic compounds contents of bread . -
pubmed
Breads incorporated with
GP presented a richer volatile profile than CB , especially due to the presence of
terpenes . -
pubmed
GP added aroma compounds and
phenolic antioxidants , and seemed to be an interesting approach to enhance bread bioactivity and acceptability . -
pubmed
An efficient and facile protocol for the synthesis of
quinazolinone - fused tetracyclic compounds through an
iodine - mediated one -
pot cascade reaction of
2 '- bromoacetophenones with
2 - aminobenzohydrazides or
2 - aminobenzamides is reported . -
pubmed
Our recent data reveal that
LPA induces matricellular protein
CCN1 ( also known as
Cyr61 ) expression in aortic smooth muscle cells ( SMCs ) and that
CCN1 bridges
LPA and
integrin signaling pathways leading to SMC migration . -
pubmed
Whether and how
LPA regulates the transcriptional machinery of the
CCN1 gene are unknown . -
pubmed
In this study , we found that
LPA markedly induces
CCN1 mRNA expression in SMCs . -
pubmed
Luciferase assays of a series of deleted , mutated
CCN1 promoter - reporter gene constructs and dominant negative construct revealed the distal SRE and the proximal CRE in the
CCN1 promoter are required for
LPA - induced
CCN1 gene expression . -
pubmed
Our results imply that elevated
LPA levels may trigger SMC migration and exacerbate
restenosis and
atherosclerotic lesions through the induced
CCN1 , which communicates with a set of plasma membrane proteins and intracellular kinases . -
pubmed
OBJECTIVE : To determine the effect of lipid /
heparin versus saline infusion , with or without concurrent euglycemic
hyperinsulinemia , on
serum follicle - stimulating hormone (
FSH ) and
luteinizing hormone (
LH ) . -
pubmed
It was hypothesized that acutely elevated
fatty acids and
insulin would impair
gonadotropin secretion . -
pubmed
Myo - inositol ( MI ) , the precursor of the second messenger
phosphoinositide (
PI ) , mediates multiple cellular events . -
pubmed
Moreover , we found that concentrations of
7 - oxo - DHEA positively correlated with absolute
CD4 + T cell counts , nadir
CD4 + T cell values and with individuals who presented
TB restricted to the lungs . -
pubmed
Mitomycin C (
MMC ) has been utilized for its antifibrotic properties in nonmalignant
tracheobronchial stenosis but its application is not well studied in
post - lung transplant
stenosis . -
pubmed
B cell depletion with the anti -
CD20 - antibody
rituximab is widely considered treatment of choice for long - term immunotherapy in
aquaporin-4 (
AQP4)-antibody positive
neuromyelitis optica spectrum disorder (
NMOSD ) . -
pubmed
In these cases , the
IL6 (
interleukin-6 )- receptor blocking antibody
tocilizumab has been suggested as an alternative . -
pubmed
The results indicated that the highest retention of total phenolic content (
TPC ) and total
flavonoid content ( TFC ) was obtained after drying at 80 C. Six
phenolic compounds were identified and quantified by liquid chromatography . -
pubmed
Pulmonary fibrosis was evaluated by
Picrosirius red staining , immunohistochemistry for
alpha - SMA and
TGF - beta1 , and
hydroxyproline assay and RT - qPCR for
Col1alpha1 and
Col3alpha1 . -
pubmed
The recombinant
FcCCD1A enzyme showed specificity for the 9,10 ( 9',10 ' ) double bond position of
cyclic carotenoids to generate
alpha - ionone and
beta - ionone , while
FcCCD1B cleaved
lycopene and an acyclic moiety of
delta - carotene , producing
6 - methyl-5 - hepten-2 - one . -
pubmed
We hypothesized that
vorinostat , a
histone deacetylase (
HDAC ) inhibitor with antiangiogenic effects , would prevent acquired resistance to
bevacizumab . -
pubmed
Multi - DNA -
Ag nanoclusters : reassembly mechanism and sensing the change of
HIF in cells . -
pubmed
Traditional Chinese medicine plays a cholagogic role mainly by promoting bile secretion , regulating
SCP2 mRNA ,
FXR ,
BSEP and efflux transporter protein , dissolving
cholesterol , promoting the relaxation of Oddi 's sphincter and changing the composition of bile , etc . -
pubmed
Enhanced Electrochemical Characteristics of the
Glucose Oxidase Bioelectrode Constructed by
Carboxyl - Functionalized Mesoporous
Carbon . -
pubmed
This research revealed the effect of
carboxyl - functionalization on the mesoporous
carbon ( MC)-fixed
glucose oxidase (
GOx ) for promoting the properties of bioelectrodes . -
pubmed
Furthermore , the GOx immobilization and
enzyme activity in
MC - COOH increased 140.72 % and 252.74 % , leading to the enhanced electroactive
GOx surface coverage of
Nafion / GOx /
MC - COOH electrode ( 22.92 % higher , 1.29 x 10 - 8 mol cm-2 ) than the control electrode . -
pubmed
1 - Deoxydihydroceramide causes anoxic
death by impairing
chaperonin - mediated protein folding . -
pubmed
SOCC is activated by depletion of
Ca2 + stores in the sarco / endoplasmic reticulum , which communicates with plasma membrane SOCC via the
Ca2 + sensor
Stromal Interaction Molecule 1 (
STIM1 ) . -
pubmed
Orai proteins alone can form highly
Ca2 + selective SOCC channels . -
pubmed
Also , members of the Transient Receptor Potential Canonical ( TRPC ) channel family are proposed to form heteromeric complexes with
Orai1 subunits , forming SOCC with low
Ca2 + selectivity . -
pubmed
Recently ,
Ca2 + entry through
SOCC , known as store - operated
Ca2 + entry ( SOCE ) , was identified in glomerular mesangial cells , tubular epithelium , and renovascular smooth muscle cells . -
pubmed
The discovery of the
EML4 -
ALK fusion gene in a limited subset of patients affected by
NSCLC and the subsequent clinical development of
crizotinib in 2011 has been an impressive milestone in
lung cancer research . -
pubmed
Afterward , modern
tyrosine kinase inhibitors ( TKIs ) , such as
ceritinib ,
alectinib ,
brigatinib , and
lorlatinib , have been approved by the Food and Drug Administration ( FDA ) for the management of
anaplastic lymphoma kinase ( ALK )- positive NSCLCs . -
pubmed
Oyster EF resident communities were predicted to be enriched for dissimilatory
nitrate reduction ,
nitrogen fixation , nitrification , and
sulfite reductase genes . -
pubmed
The expression levels of most
ThSOS genes significantly changed after
NaCl ,
PEG6000 , and
abscisic acid (
ABA ) treatment in at least one organ . -
pubmed
Together , these data suggest that overexpression of
ThSOS3 confers salt stress tolerance on plants by enhancing antioxidant enzyme activity , improving ROS - scavenging capability , and decreasing the
MDA content and lipid peroxidation of cell membranes . -
pubmed
FORMATION OF H - addition radicals in
adenine derivatives : part
II . -
pubmed
P1 - Adenosine 5 '- P2 - o - nitrobenzyl pyrophosphate (
nbzlppA ) has been synthesized as a substrate for
T4 RNA ligase catalyzed 3'-phosphorylation . -
pubmed
Incubation of
oligoribonucleotides and nbzlppA with
RNA ligase yielded
oligoribonucleotides having a
3 '- o -( o - nitrobenzyl ) phosphate . -
pubmed
A trinucleotide U - A - G was converted to
U - A - Gpnbzl and phosphorylated with
polynucleotide kinase . -
pubmed
and a
beta 1 - selective (
atenolol 50 mg o.d . ) -
pubmed
Resting saliva was assessed for flow rate ,
amylase activity and concentration of
total protein ,
hexosamine and
sialic acid . -
pubmed
Stimulated saliva was assessed for flow rate , pH , buffer pH ,
amylase activity and concentration of
total protein ,
Ca2 + ,
Mg2 + ,
Na + ,
K + ,
Cl - and
PO4 ( 2 - ) . -
pubmed
Virtually all of the
CGRP - IR postganglionic neurons exhibit colocalization with
somatostatin (
SOM ) , and , some of them with neuropeptide
tyrosine (
NPY ) . -
pubmed
[ The role of
insulin on renal
sodium handling and sympathetic nerve activity in
overweight normotensive subjects ] . -
pubmed
However , the interrelationship between
insulin ,
obesity , renal
sodium metabolism and sympathetic nerve activity in normotensive subjects (
NT ) still remains unclear . -
pubmed
The present study , therefore , was undertaken to clarify the role of
insulin on renal
sodium handling and sympatho - adrenal function in
overweight NT . -
pubmed
During the two - hour clearance period , mean arterial pressure ( MAP ) , heart rate (
HR ) , endogenous
creatinine clearance(CCr ) , urinary excretion of
sodium ( UNaV ) , fractional excretion of
sodium ( FENa ) , plasma immunoreactive
insulin ( IRI ) , plasma
norepinephrine concentration ( pNE ) , and plasma
epinephrine concentration ( pE ) were determined . -
pubmed
The stimulatory effect of
albumin on
luteinizing hormone - stimulated Leydig cell
steroid production depends on its
fatty acid content and correlates with conformational changes . -
pubmed
The pH - dependent conformational changes of
albumin and fragments thereof occurred in parallel with a change in the binding affinity for
testosterone and
pregnenolone . -
pubmed
The
fatty acid oleic acid and the drug
suramin , only when present in a molar ligand - to -
albumin ratio equal to or higher than 2 , inhibited the
albumin - mediated stimulation of
steroid production . -
pubmed
These data show that the stimulatory effects of
albumin species on
LH - induced Leydig cell
pregnenolone production depend on their
fatty acid content and correlate with the potential of these molecules to undergo conformational changes . -
pubmed
It is unknown via which mechanisms
albumin exerts its stimulatory effect , but the
LH action through the
cyclic AMP pathway seems not to be affected . -
pubmed
Inhibition of a
cell surface protease after
cisplatin chemotherapy . -
pubmed
The active centre of
GB binds the fluorescent probe
9 - aminoacridine (
9 - AA ) enabling cells containing active
GB to be visualised by fluorescent microscopy . -
pubmed
It was observed that chemotherapy with
cisplatin resulted in a marked decrease in cell surface
GB activity and this decrease was due to the formation of an enzyme - inhibitor complex . -
pubmed
Formation of a
dehydroalanyl residue from
S - benzylcysteine upon
HF cleavage of a
[ Sar1 , Cys8 ]- angiotensin II peptide resin . -
pubmed
Because
5 - HT1A receptor - mediated regulation of
5 - HT synthesis appears to be mediated by somatodendritic
autoreceptors on 5 -
HT neurons in the midbrain raphe nuclei , the results suggest that these
autoreceptors possess a large receptor reserve for agonists . -
pubmed
The pituitary
gonadotropin releasing capacity of
ketoconazole , ( KC , p.o . -
pubmed
It was found that KC resulted in a significant increase in serum
LH ( from 8.94 + /- 1.05 to 17.55 + /- 1.32 U / l ) ,
FSH ( from 6.68 + /- 1.05 to 9.82 + /- 1.28 U / l ) ,
17 - OH - progesterone ( from 4.51 + /- 0.34 to 11.35 + /- 0.68 nmol / l ) and a decrease in serum
testosterone ( from 16.62 + /- 1.28 to 11.66 + /- 1.59 nmol / l ) and
dehydroepiandrosterone ( from 6.56 + /- 0.39 to 4.82 + /- 0.37 mumol / l ) levels . -
pubmed
Furthermore , it has been established that the amount of
LH released by KC was midway between that of
GnRH and CL . -
pubmed
The same conditional pattern was observed for the concentration of cytochrome components , the membrane - associated oxidation of
NADH and
formate , and
formate phenazine methosulfate ( PMS ) reductase activity ;
succinate oxidase and
PMS reductase activities were not exhibited by membranes under any growth condition due to the suc- mutation . -
pubmed
R25PC , a spontaneous
lct+
suc- partial revertant of R25 , did not exhibit the conditional pattern of R25 . -
pubmed
Two distinct classes of R25
P1kc transductants were isolated that differed in both their growth response on
succinate and
DL - lactate and their
oxidase activities . -
pubmed
The
TPA :
B1 antibody reacted predominantly with duct cells and to a lesser extent with acinar and squamous cells . -
pubmed
The anti -
ischemic effects of
SQ 31,765 or its relatively inactive enantiomer
SQ 32,189 ( to test for effects not related to
calcium channel blockade ) were determined in a model of
stable angina . -
pubmed
SQ 32,189 , which is a 10 - fold less potent
calcium channel blocker in coronary arteries , did not affect ST - elevation ( 11.8 + /- 2.1 mV ) . -
pubmed
18O isotope exchange experiments on
phospholipase A2 determined by
13C - NMR : monomeric
phosphatidylcholine and micellar
phosphatidylethanolamine substrates . -
pubmed
Phospholipase A2 - catalyzed exchange of
H2 ( 18 ) O into
1 - alkyl-2 -[ 1 ( 13 ) C ] lauroyl - sn - glycero-3 - phosphorylethanolamine and into
1 - hexanoyl-2 -[ 1 - 13C ] hexanoyl - sn - glycero-3 - phosphorylcholine were examined . -
pubmed
The results show that there are two types of
PNMT - containing cells : those containing
PNMT exclusively and those containing
PNMT with two other
catecholamine - synthesizing enzymes ,
tyrosine hydroxylase (
TH ) and
aromatic L - amino acid decarboxylase (
AADC ) , but not
dopamine beta - hydroxylase (
DBH ) . -
pubmed
To test this hypothesis we chronically disrupted NE activity by transecting the ascending NE pathway (
ANP ) and acutely disrupted NE activity by administering the
alpha - adrenergic receptor blocking agent
phenoxybenzamine (
PBZ ) ; and we evaluated the effect of these treatments on the pattern of pulsatile
LH secretion . -
pubmed
MAGP is an acidic glycoprotein with a distinctive
amino acid composition , being exceptionally rich in
glutamic acid , rich in
cystine , and low in
glycine . -
pubmed
MAGP was extractable from tissue homogenates using
NaCl ,
urea , or
guanidine hydrochloride solutions , only if a strong reducing agent was present . -
pubmed
Thus ,
disulfide bonding is important for the strong association of
MAGP with elastic fibers . -
pubmed
The vasorelaxation to
ANF is associated with elevations of
cyclic GMP via an activation of the particulate form of
guanylate cyclase . -
pubmed
One , containing the closely related
PD1 and PD14 genes , encodes the classical transplantation
antigens . -
pubmed
SBF was estimated by local clearance of
133Xenon registered by portable
cadmium telluride detectors . -
pubmed
Cancer risk appears to be associated primarily with low concentrations of total
cholesterol and of
low - density lipoprotein cholesterol but not of
high - density lipoprotein cholesterol (
HDL ) . -
pubmed
Significant
carotene -
HDL relationships were observed among black men ( r = 0.31 ; P less than 0.05 ) and among women ( r = 0.35 ; P less than 0.05 and r = 0.31 ; P less than 0.05 , black and white , respectively ) . -
pubmed
Furthermore , the women also demonstrated a significant
carotene -
HDL2 association . -
pubmed
From 128 pronuclear eggs , each injected with 180 or 770 cubic microns of
bovine gamma globulin at a concentration of 25 milligrams per milliliter in
citrate - Locke 's
solution and then transferred to the oviducts of pregnant foster mothers , 18 living fetuses developed ; from 74 eggs , each injected with 2730 cubic microns , 5 fetuses survived . -
pubmed
There was a significant inverse correlation between plasma
bromocriptine concentration and total BPRS score at 60 minutes
post -
bromocriptine administration . -
pubmed
This late
CCRA - induced right coronary vasoconstriction was not potentiated by
beta - adrenergic receptor blockade but was abolished ( P less than 0.01 ) by
alpha - adrenergic receptor blockade . -
pubmed
Antibodies to three major antigens of the non -
histone or
saline - extractable nuclear antigen (
ENA ) complex were sought by counterimmunoelectrophoresis ( CEP ) in three groups of sera which gave different patterns in the immunofluorescence test for antinuclear antibodies ( ANA ) . -
pubmed
An enzyme immunoassay ( EIA ) , specific for
anti - SS - B was more sensitive than
CEP and identified this antibody in 28 sera , compared with 18 for
CEP . -
pubmed
The increased release and intracellular concentration of
BCAA was supported by decreased
BCAA transaminase activity in the wounded muscle tissue . -
pubmed
Non - REMS was not influenced by 3 , 30 , or 300 pmol
PACAP Sleep and
Tbr were not influenced by 3 or 30 pmol
PACAP injected at light onset . -
pubmed
The first endocrine event of puberty is an increase in the amplitude of
gonadotropin pulses at night due to increased pulsatile release of hypothalamic
gonadotropin - releasing hormone (
GnRH ) into the hypothalamic - pituitary portal system . -
pubmed
Gonadal
steroids are produced due to increased
gonadotropin stimulation , and secondary sexual development follows a well - described pattern of changes . -
pubmed
On the other hand , replacing
Gly38 with
alanine or
serine results in striking decreases in kcat to 5 and 0.6 % , respectively , of the value observed for the wild - type
enzyme , as well as in considerable increases in Km values . -
pubmed
We conclude that
Gly38 is probably required for proper function of the
enzyme because it permits a high level of flexibility for the 36 - 39 peptide , which in turn allows the essential substrate - induced movement of the small domain . -
pubmed
Bis azo dyes -- studies on the mechanism of complex formation with
IgG modulated by heating or antigen binding . -
pubmed
The mechanism of binding of
azo dyes (
bis azo ) to
immunoglobulin G of altered conformation , induced by heating or interaction with antigen was analysed in this work . -
pubmed
The peptide loop composed of
amino acids 68 - 88 originating from VL
IgG domain , was selected to represent
protein . -
pubmed
The future of
NaF should involve cyclical administration , in cautious initial dosage ( 50 - 60 mg / day ) of enteric - coated
NaF , in conjunction with a potent inhibitor of resorption such as hormone replacement ,
bisphosphonates or
calcitonin . -
pubmed
Assessment of
plasma renin activity ( PRA ) related to urinary
sodium excretion was used to define subgroups with high ( n = 12 ) , medium ( n = 16 ) and low
renin profiles ( n = 8) . -
pubmed
Glucose ,
insulin and
C - peptide responses to standard oral
glucose tolerance test ( OGTT ) were measured . -
pubmed
HDL -
cholesterol levels were lower in the high
renin group than in the low
renin group ( 1.05 + /- 0.04 versus 1.26 + /- 0.09 mmol L-1 , P & lt ; 0.05 ) . -
pubmed
In the
amide I , clear changes in the bands at 1666 cm-1 , assigned to alpha II helices , 1659 cm-1 , assigned to alpha I and alpha
II helices , and 1652 cm-1 , assigned to both alpha I helices and unordered structures , were found . -
pubmed
The side chains of
Tyr ( band at 1517 cm-1 ) and
Phe ( band at 1498 cm-1 ) were found to change in going from
BR to N. In the
carboxylate region , no band was detected at 1737 cm-1 in the deconvoluted spectra that could correspond to the peak observed in the difference spectrum . -
pubmed
The significant case - control difference in
LDL size was independent of levels of
high - density lipoprotein cholesterol (
HDL - C ) , non -
HDL cholesterol ( non -
HDL - C ) ,
triglyceride , smoking ,
systolic blood pressure , and
body mass index , but was not significant after adjusting for the ratio of total
cholesterol ( TC ) to
HDL - C ( TC :
HDL - C ) . -
pubmed
A fragment containing
C5 - C7 also blocks uptake and degradation of
125I - RAP by fibroblasts in a concentration - dependent manner . -
pubmed
Binding competition experiments show that
RAP ,
PAI-1 , and
lactoferrin each inhibit the binding of the others , suggesting that at this site in
LRP ,
RAP acts as a competitive , rather than an allosteric , inhibitor of
PAI-1 and
lactoferrin binding . -
pubmed
Replacement of
Na + with
K + in the acidified medium canceled the effect of
EGF . -
pubmed
Indomethacin and
W-7 ( a
Ca - calmodulin inhibitor ) did not alter the protective effect of
EGF . -
pubmed
In contrast ,
genistein ( a
tyrosine kinase inhibitor ) ,
amiloride ( a
Na +/ H + exchangers I and II inhibitor ) , and
wortmannin ( a
phosphatidylinositol 3 - kinase inhibitor ) significantly decreased the effect of
EGF . -
pubmed
GM - CSF levels were measured by ELISA and
melatonin levels by radioimmunoassay ( RIA ) . -
pubmed
We also analyzed the activity of
calpain I ,
calpain II , and
calpastatin by the conventional
DEAE chromatographic method for comparison . -
pubmed
The results indicated that the
RPA is more repeatable than the
DEAE method . -
pubmed
Special features of the RPA as compared with
DEAE chromatography are as follows ; 1 ) the RPA is a reliable method for quantifying the expression of calpains in all tissues because it is not affected by the presence of inhibitors or activators , 2 ) the RPA method can be expanded to analyze the expression of the tissue - specific calpains simply by designing specific probes for them , and 3 ) the
RPA requires a small amount of tissue . -
pubmed
Mitogen - activated protein kinase and
abscisic acid signal transduction . -
pubmed
It was found that
ABA signal transduction in plants can involve the activity of
type 2C - phosphatases (
PP2C ) ,
calcium ,
potassium , pH and a transient activation of
MAP kinase . -
pubmed
However ,
type 2C phosphatases are involved in the induction of most
ABA responses , as shown by the
PP2C - deficient
abi - mutants . -
pubmed
In addition , the role of
farnesyl transferase as a negative regulator of
ABA responses also indicates towards involvement of
MAP kinase in
ABA signal transduction . -
pubmed
Further establishment of the involvement of
MAP kinase in
ABA signal transduction and its role therein , still awaits more study . -
pubmed
In one of the cell lines ( Mut F ) , two unmethylated
CpG islands in the double minute chromosomes are readily cleaved by methylation - sensitive rare - cutting
restriction endonucleases . -
pubmed
The partially methylated
CpG islands can be demethylated by
azacytidine treatment or naturally by extended time in culture , and become sensitive to cleavage with the rare - cutting
restriction endonucleases . -
pubmed
Whole blood chemiluminescence ( CL ) , which allows for the evaluation of both the
oxygen radical release by phagocytes and the
serum opsonin activity using very small amounts of blood , is considered to be a useful means of assessing the host defense against
infection . -
pubmed
As early as 11 days after C225 treatment , the median percentage of
proliferating cell nuclear antigen - positive cells was substantially reduced compared with
gemcitabine treatment alone ( 26 % versus 73 % , respectively ) versus controls ( 92 % ) , correlating with in vivo blockade of
EGF - R activation . -
pubmed
Roles of
A beta and
carboxyl terminal peptide fragments of
amyloid precursor protein in
Alzheimer disease . -
pubmed
We focused on the amyloidegenic
carboxyl terminal fragments of
betaAPP containing the full length of
A beta ( CT105 ) . -
pubmed
Our results suggest that the cytotoxic and channel inducing effects of
CT105 are much more potent than that of A beta and toxic mechanisms of
CT105 are different from those of A beta . -
pubmed
It is known that
nitric oxide (
NO ) influences the activities of
glycolytic enzymes , resulting in alteration of glycolysis rate . -
pubmed
RESULTS :
Glibenclamide infusion had a high proarrhythmic action ( ASI :
glibenclamide 143 , control 54 and vehicle 23 ; ANOVA
P & lt ; 0.001 drug vs. control and vehicle ) and a detrimental effect on regional systolic ( % WTH : glibenclamide 26.9 + /- 6.7 , control 65.7 + /- 3.5 and vehicle 68.6 + /- 5.6 , ANOVA P & lt ; 0.01 drug vs. control and vehicle ) and diastolic function ( CR : glibenclamide 76.2 + /- 7.8 , control 104.7 + /- 4.2 and vehicle 106 + /- 4.9 , ANOVA P & lt ; 0.05 drug vs. control and vehicle ) during reperfusion . -
pubmed
Structural requirements of
sphingosine molecules for inhibition of
DNA primase : biochemical and computational analyses . -
pubmed
Using 28 chemically well - defined compounds containing
D - erythro - sphingosine and its analogues , we analyzed structure - activity relationships for
DNA primase inhibition . -
pubmed
Biochemical studies demonstrated a positively charged
amino group at
C2 and a long aliphatic chain to be absolutely required for inhibition . -
pubmed
This result could be due to cancellation of positive charge of the
amino group by the interaction with negatively charged
C1 - phosphate , since simulations with the software INSIGHT
II showed these two groups to be close enough to interact . -
pubmed
The
hydroxyl group at
C3 and trans - double bond at
C4 -
C5 were also found to be important for the inhibition . -
pubmed
Despite saturation of the double bond , introduction of a
hydroxyl group into
C4 of
dihydrosphingosine resulted in restoration of inhibition . -
pubmed
Modifications such as deletion of the
hydroxyl group at C3 or saturation of the
C4 - C5 double bond caused shifts in the dihedral angle of
C2 - 3 - 4 - 5 , with concomitant decrease in inhibitory activity . -
pubmed
The AF10 gene encodes a putative
transcription factor containing an N - terminal
LAP / PHD zinc finger motif , a functional nuclear localization signal , an AT - hook domain , and a
leucine zipper toward the C - terminus . -
pubmed
The chimeric fusion proteins
MLL / AF10 and
CALM /
AF10 , resulting from the t(10;11)(p12;q23 ) and the t(10;11)(p12;q14 ) , respectively , consistently retain the
leucine zipper motif of
AF10 . -
pubmed
The
leucine zipper interacted with
GAS41 , a protein previously identified as the product of an amplified gene in a
glioblastoma . -
pubmed
The retention of the
leucine zipper in the
MLL and
CALM fusions suggests that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the
adenosine triphosphate - dependent chromatinremodeling complexes . -
pubmed
The effects of
GTP gamma S could be antagonised by pre - treatment of hyphae with
GDP beta S , a non - phosphorylatable form of
GDP . -
pubmed
This could be a
dynamin - like
GTP - ase , since
GTP gamma S - stimulated tubule formation has only been reported previously in cases where a
dynamin is involved . -
pubmed
Brefeldin A antagonised
GTP gamma S , and inhibited tubule formation in the tip cell . -
pubmed
However ,
Exendin-4 treatment prevented
hyperglycaemia ( fasting : 6.1+/-1.0 mmol / l , p<0.01 ) , with reduced plasma
insulin concentrations ( p<0.001 ) and improved
glucose tolerance ( p<0.05 ) . -
pubmed
Regulation of
equine lymphocyte beta - adrenoceptors under the influence of
clenbuterol and
dexamethasone . -
pubmed
for 12 days , decreased significantly
ICYP binding sites by approximately 30 - 40 % ; concomitantly , lymphocyte
cAMP response to ( -)-
isoprenaline was reduced . -
pubmed
After withdrawal of
clenbuterol ,
beta2 - adrenoceptor density and responsiveness gradually increased , reaching predrug levels after 4 days . -
pubmed
for 5 days ) immediately after
clenbuterol withdrawal accelerated
beta2 - adrenoceptor recovery : only 24 h after administration
dexamethasone restored the number of
binding sites and
cAMP response to ( -)-
isoprenaline to levels statistically indistinguishable from values before
clenbuterol treatment . -
pubmed
Three days after
dexamethasone administration ,
lymphocyte beta2 - adrenoceptors were further increased about 2 - fold the pretreatment values , and this increase declined gradually after
dexamethasone withdrawal , reaching baseline values after 4 days . -
pubmed
Furthermore , in groups exposed simultaneously to both drugs ,
dexamethasone completely prevented
clenbuterol - induced decrease in lymphocyte
beta2 - adrenergic receptor density and responsiveness . -
pubmed
METHODS :
Polytetrafluoroethylene (
PTFE ) grafts pretreated with
fibronectin were seeded with SMC followed by ECs one day later and exposure to an in vitro flow system . -
pubmed
Several studies have attempted to use biomarkers of biologically effective dose ( BBED ) such as
polycyclic aromatic hydrocarbon - DNA and
alfatoxin -
albumin adducts to assess possible GxE interactions . -
pubmed
[ Effectiveness of
post - tonsillectomy aerosol
penicillin therapy ] . -
pubmed
Although it has been demonstrated exhaustively that potent and structurally diverse
ATP - antagonistic small molecule
kinase inhibitors can be found through mass screening and structure - guided design , the question of biochemical , cellular , and in vivo selectivity of such inhibitors remains much less clear . -
pubmed
The
adenosine A ( 2A ) receptor antagonist ,
1,3,7 - trimethyl-8 -( 3 - chlorostyryl ) xanthine ( 0.5 or 1.0 mg / kg , i.p . ) -
pubmed
, significantly reversed the pharmacological action of
PAP 9704 in a dose related manner , but the
adenosine A ( 1 ) receptor antagonist
8 - cyclopentyl-1,3 - dimethylxanthine ( 0.5 or 1.0 mg / kg , i.p . ) -
pubmed
and the
A ( 2B ) receptor antagonist
alloxazine ( 1.5 or 3.0 mg / kg , i.p . ) -
pubmed
Our results suggest that
PAP 9704 prevents MA - induced
hyperlocomotion , at least in part , via the stimulation of the
adenosine A ( 2A ) receptor . -
pubmed
Because mutations in both receptor genes appear to affect some parameters of cardiac function and because cardiac functional activity and energy metabolism are linked , we measured heart
glucose utilization ( HMR(
Glc ) ) in both the
TRbeta(
PV /
PV ) and
TRalpha(
PV/+ ) mutants . -
pubmed
6 -( 2 - Furanyl )- 9H - purin-2 - amine derivatives as
A2A adenosine antagonists . -
pubmed
Structure - activity relationships have been investigated through substitutions at the 9 - position of the
2 - amino-6 -( 2 - furanyl ) purine ( 5 ) to identify novel and selective
A ( 2A ) adenosine receptor antagonists . -
pubmed
Neither ovariectomy nor chronic treatment with
melatonin affected plasma concentrations of
leptin or the circadian pattern of secretion . -
pubmed
Comparing different inducers , the best protection was achieved with
BABA followed by
jasmonic acid ( JA ) , whereas
benzo ( 1,2,3 )- thiadiazole-7 - carbothionic acid - S - methyl ester ( a
salicylic acid [
SA ] analog ) and
abscisic acid (
ABA ) treatment did not increase the resistance significantly . -
pubmed
Marker genes for the
SA and JA pathways showed potentiated expression patterns in
BABA - treated plants following
infection . -
pubmed
Application of the
phenylalanine ammonia lyase inhibitor
2 - aminoindan-2 - phosphonic acid and the
lipoxygenase (
LOX ) inhibitor
5 , 8 , 11 , 14 - eicosatetraynoic acid (
ETYA ) also led to a reduction of
BABA - induced resistance ( BABA - IR ) , suggesting that callose deposition as well as defense mechanisms depending on
phenylpropanoids and the JA pathways all contribute to
BABA - IR . -
pubmed
The results strongly suggest that the initial oxidation of
PVA is mediated via a
PVA oxidase , and not a
PQQ - dependent
dehydrogenase . -
pubmed
Clinical outcomes after multivessel versus single - vessel percutaneous coronary intervention ( PCI ) in the
era of stents ,
glycoprotein IIb / IIIa inhibitors , and
clopidogrel pretreatment have not been well studied . -
pubmed
Prolactin production in these extrapituitary sites is not governed by
dopamine agonists , a finding that has spurred the production of
prolactin receptor antagonists . -
pubmed
The
kappa - opioid receptor agonist
nalfurafine suppressed
herpetic and postherpetic pain to similar degrees . -
pubmed
These results suggest that specific down - regulation of the
mu - opioid receptor in the primary sensory neurons is responsible for the reduced analgesic action of
morphine on
postherpetic pain . -
pubmed
Apoptotic morphology was completely prevented by the non - selective
caspase inhibitor
z - VAD - fmk , indicating an important role for
caspases in neurodegeneration induced by street
heroin . -
pubmed
Nonetheless , in street
heroin - treated cortical neurons ,
cytochrome c was released , accompanied by a decrease in mitochondrial potential and
Bcl-2 /
Bax . -
pubmed
Pure
heroin hydrochloride similarly decreased metabolic viability but only slightly activated
caspase 3 . -
pubmed
The phosphorylation of
Akt occurred downstream of
PI3 K and
phospho -
Akt was found to accumulate in the nucleus of
IGF-1 - treated cells . -
pubmed
Tetrahydrobiopterin shows chaperone activity for
tyrosine hydroxylase . -
pubmed
Tyrosine hydroxylase (
TH ) is the rate - limiting enzyme in the synthesis of
catecholamine neurotransmitters . -
pubmed
Primary inherited defects in
TH have been associated with
l - DOPA responsive and non - responsive
dystonia and
infantile parkinsonism . -
pubmed
In this study , we show that both the cofactor
( 6R )- l - erythro-5,6,7,8 - tetrahydrobiopterin (
BH ( 4 ) ) and the feedback inhibitor and
catecholamine product
dopamine increase the kinetic stability of
human TH isoform 1 in vitro . -
pubmed
Our results also establish that orally administered
BH ( 4 ) crosses the blood - brain barrier and therapeutic regimes based on
BH ( 4 ) supplementation should thus consider the effect on
TH . -
pubmed
OBJECT : Transvenous embolization ( TVE ) for the treatment of a cavernous
sinus (
CS )
dural arteriovenous fistula (
DAVF ) occasionally causes
cranial nerve palsy (
CNP ) . -
pubmed
INTRODUCTION : The action of
cortistatin (
CST ) , a novel cyclic neuropeptide , as an anti - inflammatory factor has been studied , but few investigations have explored the immunomodulatory role of
CST in transplantation . -
pubmed
SMS 201 - 995 at the same concentrations did not have a significant effect on allograft survival (
MST , 8 days for both groups ) . -
pubmed
AIM : This was to carry out a review of the literature concerning
mineral trioxide aggregate ( MTA ) and Portland cement with regards to clinical , biological and mechanical findings and a possible substitution of
MTA through Portland cement for endodontic use . -
pubmed
Acyl - HSLs are synthesized by the
LuxI family of synthases , and the signal response is mediated by members of the
LuxR family of transcriptional regulators . -
pubmed
thailandensis quorum - sensing mutants , and we used these mutants to show that
BtaI1 is responsible for C(8)-HSL production and
BtaI3 is responsible for
3OHC ( 8 )- HSL production . -
pubmed
Importantly , we show that the BaffrDelta168 - 175 deletion mutant lacking the retrotransposon - displaced residues , and a
BaffrT170A mutant lacking a critical
threonine , supported B cell survival but failed to support optimal
CD21 / 35 expression . -
pubmed
Namely , pressurized liquid extraction ( PLE ) , using water and
ethanol as solvents , supercritical fluid extraction (
SFE ) , using neat
CO ( 2 ) and supercritical
CO ( 2 ) modified with
ethanol , as well as a novel extraction process called Water Extraction and Particle formation On - line ( WEPO ) are directly compared . -
pubmed
Glyoxalase I [
lactoylglutathione lyase (
EC 4.4.1.5 ) encoded by
GLO1 ] is a ubiquitous cellular defense enzyme involved in the detoxification of
methylglyoxal , a cytotoxic byproduct of glycolysis . -
pubmed
Accumulative evidence suggests an important role of
GLO1 expression in protection against
methylglyoxal - dependent protein adduction and cellular damage associated with
diabetes ,
cancer , and chronological aging . -
pubmed
Using two - dimensional proteomics followed by mass spectrometry the
methylglyoxal - adducted protein was identified as
heat shock protein 27 (
Hsp27 ;
HSPB1 ) . -
pubmed
Taken together , our data suggest a function of
GLO1 in the regulation of detoxification and target adduction by the glycolytic byproduct
methylglyoxal in
malignant melanoma . -
pubmed
In this paper , a chitosan -
methotrexate covalently conjugated nanoparticles (
CS -
MTX -
TPP NPs ) has been developed as a potential delivery system for
methotrexate (
MTX ) . -
pubmed
MTX was first conjugated to
CS by using
glutaraldehyde as cross - linked agent , and followed by the process of ionic gelation between
MTX - conjugated
CS and
sodium tripolyphosphate (
TPP ) to form
CS -
MTX -
TPP NPs at mild reaction conditions . -
pubmed
The hydrodynamic diameter of
CS -
MTX -
TPP NPs and the encapsulation efficiency of
MTX were affected by the weight ratio of
MTX /
CS . -
pubmed
At the ratio of 1/11 , the mean hydrodynamic diameter of
CS -
MTX -
TPP NPs was the lowest ( 187.9 + /- 9.0 nm ) and the nanoparticles presented the encapsulation efficiency of
MTX with 53.0 + /- 3.2 % . -
pubmed
The characterizations by atomic force microscopy (
AFM ) and photon correlation spectroscopy ( PCS ) showed the
CS -
MTX -
TPP NPs had a spherical shape and good dispersion with diameter of sub-200 - nm and zeta potential of 30 mV. -
pubmed
Additionally , in vitro release test revealed that the stable covalent bonding of
CS and
MTX was beneficial for providing slow release for
MTX . -
pubmed
Extremely accurate X - ray data were obtained for the explosive
RDX (
hexahydro-1,3,5 - trinitro-1,3,5 - triazine ) at three different temperatures ( 20 , 120 and 298 K ) . -
pubmed
For
1,3,5,7 - tetranitro-1,3,5,7 - tetraazacyclooctane (
HMX ) , old data collected at 20 and 120 K were re - integrated with the new version of VIIPP and refined in the same manner as for
RDX . -
pubmed
Limiting the refinement to a harmonic model of the atomic displacements may result in a biased and erroneous electron density , especially when atomic vibrations are significant ( as in
RDX ) and at temperatures higher than obtained by using liquid
helium . -
pubmed
For
RDX it was observed that the
oxygen lone - pair concentrations of electrons are located close to perpendicular to the
N - O bond vectors , which is typical for explosive materials . -
pubmed
Blocks from 27 necropsy cases of
prostatic carcinoma were sectioned and stained with H+E , fifteen biotinylated
lectins chosen to probe for a wide range of
oligosaccharide sequences within several categories of glycoprotein glycans , using a
lectin - biotin avidin - peroxidase method , and monoclonal antibodies against
Lewisa ,
sialyl Lewisa and
sialyl Lewisx antigens . -
pubmed
For discovery and replication we used more than 3,300 independent individuals from KORA
F3 and F4 with metabolite measurements of 131 metabolites , including
amino acids ,
phosphatidylcholines ,
sphingomyelins ,
acylcarnitines , and
C6 - sugars . -
pubmed
Sex - specific genome - wide association studies ( GWAS ) showed genome - wide significant differences in beta - estimates for SNPs in the
CPS1 locus (
carbamoyl - phosphate synthase 1 , significance level : p<3.8x10(-10 ) ; Bonferroni - corrected threshold ) for
glycine . -
pubmed
Cytoskeletal changes and phosphorylation events mediated by
Fyn activity were reversed by
TM4SF10 overexpression , including a decrease in the activating
tyrosine phosphorylation of
Fyn ( Y(421 ) ) , suggesting
TM4SF10 may have a regulatory role in suppressing
Fyn activity . -
pubmed
In addition ,
TM4SF10 was reexpressed following podocyte
injury by
puromycin aminonucleoside treatment , and its expression enhanced the abundance of high - molecular - weight forms of
nephrin indicating it may participate in a mechanism controlling
nephrin ' s appearance at the plasma membrane . -
pubmed
The most potent
PDE5 inhibitor was
5,7 - dimethoxyflavone ( IC(50 ) = 10.64 + - 2.09 muM , selectivity on
PDE5 over
PDE6 = 3.71 ) . -
pubmed
Structure activity relationship showed that the
methoxyl group at C-5 position of
7 - methoxyflavones was necessary for
PDE5 inhibition . -
pubmed
Moreover ,
5,7 - dimethoxyflavones should make a useful lead compound to further develop clinically efficacious
PDE5 inhibitors . -
pubmed
In this work , a novel TSMP system comprised of both permanent covalent cross - links and supramolecular
hydrogen bonding cross - links has been synthesized via a one -
pot method . -
pubmed
The in vivo role of
nuclear receptor corepressors in
thyroid hormone action . -
pubmed
No significant decreases in the levels of
GSH or total antioxidation capacity were found in the
SR X - ray - irradiated testes . -
pubmed
However , the
SR X - ray at 40 Gy induced a marked increase in phosphorylated
H2AX - a marker of double - strand DNA damage , which was significantly decreased by the antioxidant
N - acetyl cysteine (
NAC ) . -
pubmed
NAC also attenuated the
SR X - ray - induced decreases in the cell layer number of seminiferous tubules . -
pubmed
A skincare containing
retinol adenosine and
hyaluronic acid optimises the benefits from a type A
botulinum toxin injection . -
pubmed
SPA showed a positive correlation with high - sensitive
C - reactive protein and a negative correlation with
high - density lipoprotein cholesterol levels ( r = 0.313 , p = 0.021 and r = -0.233 , p = 0.036 , respectively ) . -
pubmed
A combination of conventional
TEM and PCR imaging allows one to detect complementary transmission and topographical information with nanometer precision of the same area of
carbon - based samples . -
pubmed
Functional gene categorization showed that genes associated with
salicylic acid ( SA)-associated defenses , signal transduction , transport , and the secretory machinery are overrepresented in the group of
SAR(+ ) genes , and that the group of
SAR(- ) genes is enriched in genes activated via the
jasmonate ( JA)/
ethylene ( ET)-defense pathway , as well as in genes associated with cell wall remodeling and biosynthesis of constitutively produced secondary metabolites . -
pubmed
Cluster
II genes can be expressed independently of
SA , and this group is moderately enriched in
H2O2- and
abscisic acid (
ABA)-responsive genes . -
pubmed
In WHR ,
relaxin stimulated flow - mediated vasodilation ( 2.67 fold , from 48 + - 9 to 18 + - 4 mul / min , p = 0.001 ) , inhibited myogenic reactivity ( from -1 + - 2 to 7 + - 3 mum/10 mmHg , p = 0.01 ) , and decreased sensitivity to
phenylephrine ( 28 % , from 1.39 + - 0.08 to 1.78 + - 0.10 muM , p<0.01 ) , but left compliance and RPFF unchanged . -
pubmed
NO - blockade by
L - NAME diminished most
relaxin - mediated responses . -
pubmed
In SHR , the vasodilator effects of
relaxin were blunted for myogenic reactivity and sensitivity to
phenylephrine , with similar effects on flow - mediated vasodilation , compliance , RPFF and equal
Rxfp1 (
relaxin family peptide receptor ) gene expression , as compared to WHR . -
pubmed
Three independent
glc1 mutations were shown to have the same pleiotropic phenotype :
catalase T deficiency , defective glycogen synthesis and defective
trehalose accumulation . -
pubmed
Following 1 - week
rosiglitazone (
Rosi ) therapy , urinary
PGE2 , but not other
prostanoids , was reduced by 57 % in parallel with significant reduction of
mPGES-1 protein and
EP4 mRNA expressions . -
pubmed
In line with the reduction of glomerular
mPGES-1 , the
glomerular injury score showed a tendency of improvement after 1 week of
Rosi therapy . -
pubmed
Collectively , the present study demonstrated an inhibitory effect of
PPAR gamma activation on renal
mPGES-1 /
PGE2 /
EP4 pathway in
type-2 diabetes and suggested that
mPGES-1 may potentially serve as a therapeutic target for treating
type-2 diabetes - associated
DN . -
pubmed
Control of
NF - kB activity in
human melanoma by bromodomain and extra - terminal protein inhibitor I -
BET151 . -
pubmed
We show that I -
BET151 is a potent , selective inhibitor of a number of
NF - kB target genes involved in induction of
inflammation and cell cycle regulation and downregulates production of
cytokines such as
IL-6 and
IL-8 . -
pubmed
A2B adenosine receptor stimulation decreased phosphorylation of
extracellular signal - regulated kinases 1 and 2 (
ERK1 / 2 ) and
stress - activated protein kinase /
Jun - amino - terminal kinase (
SAPK /
JNK ) at all three
O2 concentrations . -
pubmed
Adenosine receptor A2B stimulation decreased
VEGF expression at 2 % and 8 %
O2 but led to increased levels at 21 %
O2 . -
pubmed
RESULTS : After adjusting for age , sex , ethnicity ,
vitamin D , waist circumference , family poverty income ratio , total
cholesterol , ever
asthma ,
total protein , and survey weighting , abnormal
gamma glutamyl transpeptidase was significantly associated with
food sensitisation ( peanut : OR 2.17 , 95%CI 1.60 - 2.94 , P<0.001 ; egg : OR 2.55 , 95%CI 1.32 - 4.90 , P=0.008 ; milk : OR 2.59 , 95%CI 1.56 - 4.31 , P=0.001 ; shrimp : OR 1.81 , 95%CI 1.29 - 2.55 , P=0.002 ) . -
pubmed
HG activates
MMP-9 production ,
MMP-9 activity and
MUC5AC overproduction , which is inhibited by
nPG ,
DMSO and
DPI ( inhibitors of ROS and
NADPH ) , suggesting that HG - activated mucin synthesis is mediated by
NADPH / ROS in 16HBE cells . -
pubmed
These observations demonstrate an important role for
MMP-9 activated by
NADPH / ROS signaling pathways in regulating HG - induced
MUC5AC expression . -
pubmed
Epigallocatechin-3 - gallate enhances clearance of phosphorylated
tau in primary neurons . -
pubmed
EGCG is a potent antioxidant and an activator of the
Nrf2 transcriptional pathway . -
pubmed
Nrf2 activators including
EGCG have shown promise in mitigating amyloid pathology in vitro and in vivo . -
pubmed
RESULTS :
EGCG treatment led to a significant decrease in the protein levels of three
AD - relevant
phospho -
tau epitopes . -
pubmed
Unexpectedly ,
EGCG does not appear to be facilitating this effect through the
Nrf2 pathway or by increasing autophagy in general . -
pubmed
However ,
EGCG did significantly increase mRNA expression of the key autophagy adaptor proteins
NDP52 and
p62 . -
pubmed
DISCUSSION : In this study , we show that
EGCG enhances the clearance of
AD - relevant phosphorylated
tau species in primary neurons . -
pubmed
Taken together , these results demonstrate that
EGCG has the ability to increase the clearance of phosphorylated
tau species in a highly specific manner , likely through increasing adaptor protein expression . -
pubmed
BACKGROUND : Certain
proton pump inhibitors ( PPIs ) interfere with
clopidogrel metabolism , potentially attenuating
P2Y12 receptor inhibition . -
pubmed
METHODS AND RESULTS : On - treatment
P2Y12 platelet reactivity testing was performed using the VerifyNow assay after
clopidogrel loading and successful drug - eluting stent implantation at 11 sites in the United States and Germany . -
pubmed
In particular ,
human haemochromatosis protein (
HFE ) is involved in
iron metabolism , and
HFE H63D polymorphism has been related to the risk of
amyotrophic lateral sclerosis and
Alzheimer ' s disease . -
pubmed
The
bronchospasm related with
remifentanil ,
post - ECT
bradycardia ,
hypertensive crisis and
oligohydramnios were also reported in one case each . -
pubmed
Peroxidase (
POD ) catalyzed the polymerization of naturally occurring
phenolics to give a variety of bioactive products . -
pubmed
The
guaiacol readily reacted in the
POD system and afforded potential anti - microbial compounds . -
pubmed
Secondary outcomes included the correlation of SctO2 with other relevant physiological variables , the dependence of SctO2 on baseline
hemoglobin and transfusion , and the effect of red blood cell transfusion on fractional tissue
oxygen extraction . -
pubmed
Medical therapies including anticoagulation , statin therapy , and
angiotensin - renin -
aldosterone axis antagonists may reduce
dementia risk . -
pubmed
SL Dams and MSG Dams presented an increase in WAT content and higher plasma levels of
glucose ,
triglycerides , and
insulin , in relation to NL Dams and CON Dams , respectively . -
pubmed
Therefore , we evaluated
HD cell responses to visual cue rotations in a familiar arena , and during navigation between familiar and novel arenas , after
muscarinic receptor blockade with systemic
atropine . -
pubmed
Protein arginine methyltransferase 1 (
PRMT1 ) catalyses the methylation of substrate
arginine by transferring the
methyl group from
SAM (
S - adenosyl - l - methionine ) , which leads to the formation of
S - adenosyl homocysteine (
SAH ) and
methylated arginine . -
pubmed
We have shown previously that the
Asp84 on
PRMT1 could be a potential inhibitor binding site . -
pubmed
Our results show for the first time that
PRMT1 inhibitors can target the
Asp84 binding site , which will be helpful for future drug discovery studies . -
pubmed
Levels of serum
creatinine (
Cr ) in each
EGCG - treated group were decreased but not significant ; the activities of hepatic
xanthine oxidase (
XOD ) and
adenosine deaminase (
ADA ) in high dose groups '
EGCG were notably lower than those of model group . -
pubmed
Ultrasound-(US ) propelled nanowires consisting of
Au /
Ni /
Au /
PEDOT -
PPy -
COOH segments are modified with
asparaginase enzyme and applied as an effective anti -
cancer agent . -
pubmed
The fast motion of the motor - carrying
asparaginase leads to greatly accelerated biocatalytic depletion of
asparagine and hence to a significantly enhanced inhibition efficacy against El4
lymphoma cancer cells ( 92 % ) as compared to free enzyme counterpart ( 17 % ) and other control groups . -
pubmed
BACKGROUND : Recent guidelines recommend
riociguat , a soluble
guanylate cyclase (
sGC ) stimulator , and the
type 5 phosphodiesterase inhibitor ( PDE5i )
tadalafil or
sildenafil as treatments for
pulmonary arterial hypertension . -
pubmed
These conjugates have different DNA surface densities and thus exhibit different levels of
nuclease resistance , cellular uptake , and gene regulation capabilities ; the properties displayed by the
C60 SNA conjugate are closer to those of conventional and prototypical gold nanoparticle SNAs . -
pubmed
Proline substitutions in a
Mip - like
peptidyl - prolyl cis - trans isomerase severely affect its structure , stability , shape and activity . -
pubmed
Unlike
rFKBP22 , the three mutants were unfolded by a non - two state mechanism in the presence of
urea . -
pubmed
Conversely , the
rapamycin binding affinity of no mutant was different from that of
rFKBP22 . -
pubmed
The inhibitor
[ BDA - Fe ( III ) ] was designed to possess two functional groups : the
anthraquinone moiety , as recognition element by
hGSTP1 - 1 and a metal chelated complex [
iminodiacetic acid - Fe ( III ) ] as a reactive moiety , able to generate reactive
oxygen species ( ROS ) , through Fenton reaction . -
pubmed
Upon binding of the
BDA - Fe ( III ) to
hGSTP1 - 1 in the presence of
hydrogen peroxide , reactive
oxygen species ( ROS ) are generated , which promoted the specific cleavage of
hGSTP1 - 1 in a time and concentration - dependent manner . -
pubmed
In this study , the effects of two weeks of daily subcutaneous exposure to the OP - nerve agent
diisopropylfluorophosphate (
DFP ) in doses ranging from 0.125 - 0.500 mg / kg on whole brain volume , white matter , and gray matter integrity were evaluated in
post mortem tissues using histology and magnetic resonance imaging ( MRI ) methods . -
pubmed
DFP was associated with dose - dependent impairments in red blood cell and brain
AChE ( down to 29 and 18 % of control , respectively at the highest dose ) , 24 h after the last injection . -
pubmed
Moreover , histological and MRI analysis of
post mortem brains did not reveal any pronounced alterations of whole brain , white matter , or gray matter volumes associated with
DFP . -
pubmed
OBJECTIVES : The most challenging stage of cardiac resynchronization therapy (
CRT ) is coronary
sinus cannulation (
CS ) . -
pubmed
METHODS : Twelve well - trained , male triathletes ( aged 21 - 47 yr ) were assigned in a randomized , double - blind , crossover design to receive 70 ml of BJ ( 6.5 mmol
NO3- ) or placebo (
PL ) . -
pubmed
For 40 dpf ( days
post - fertilisation ) F0 generations ,
estradiol hormone and
vitellogenin (
Vtg ) contents , the expression levels of
Vtg1 ,
P450 aromatase (
Cyp19a and
Cyp19b ) ,
17beta - hydroxysteroid dehydrogenase (
Hsd17b1 ,
Hsd17b3 ) ,
estrogen receptor - alpha and
progesterone receptor in all concentration groups decreased significantly , while
androgen receptor increased significantly in 10 and 100 mug / L exposure groups compared with the corresponding control group , showing anti -
estrogen and
androgen effects . -
pubmed
For 120 dpf F0 generations ,
acetylcholinesterase activity was significantly decreased and
glutathione and
malondialdehyde levels ,
superoxide dismutase ,
catalase and
glutathione reductase activities were significantly increased in all treatment groups compared with the corresponding control group . -
pubmed
Data on lifetime history of
alcohol use , DSM -
IV use disorders , and remission were collected from 1,459 adults using
face - to -
face interviewers based on the Composite International Diagnostic Interview ( CIDI 3.0 ) . -
pubmed
By using one of the
sulfate oxygen atoms as a third donor in addition to the bidentate
pyridinone moiety ,
BAL30072 can form a
L2 M complex , which was predicted to be the one with the best binding affinity to
PiuA. -
pubmed
Vitamin D and probiotic co - supplementation was associated with a significant reduction in total
testosterone ( beta - 0.19 ng / mL ; 95 % CI , - 0.28 , - 0.10 ; P & lt ; 0.001 ) ,
hirsutism ( beta - 0.95 ; 95 % CI , - 1.39 , - 0.51 ; P & lt ; 0.001 ) , high - sensitivity
C - reactive protein (
hs - CRP ) ( beta - 0.67 mg / L ; 95 % CI , - 0.97 , - 0.38 ; P & lt ; 0.001 ) and
malondialdehyde (
MDA ) levels ( beta - 0.25 mumol / L ; 95 % CI , - 0.40 , - 0.10 ; P = 0.001 ) , and a significant increase in total antioxidant capacity ( TAC ) ( beta 82.81 mmol / L ; 95 % CI , 42.86 , 122.75 ; P & lt ; 0.001 ) and total
glutathione (
GSH ) levels ( beta 40.42 mumol / L ; 95 % CI , 4.69 , 76.19 ; P = 0.02 ) , compared with the placebo . -
pubmed
A total of 868 FET cycles were included and randomized into two groups : Group A ( n = 434 ) received
GnRHa 0.1 mg subcutaneous injection on day 3 after embryo transfer (
ET ) ; Group B ( n = 434 ) did not receive GnRHa . -
pubmed
In conclusion , single dose of GnRHa ( 0.1 mg
triptorelin acetate ) supplementation 3 days after
ET in AC - FET cycles did not show significant benefit on pregnancy outcomes as a whole . -
pubmed
Consequently , the selected antigenic
amino acids sequences ( B - cell epitopic regions ) were analyzed for T - cell epitopes determination (
MHC I and
MHC II alleles binding sequence ) performing the ProPred 1 and ProPred server respectively . -
pubmed
albicans , whilst a low
voriconazole concentration ( 0.25 microg / mL ) blocked their interactions similar to the effect of the
erg11Delta / Delta mutant . -
pubmed
Instead , the intron - retained enzyme
BGL1B had a better thermostability with a higher optimal temperature and a longer half - life under 50 C. Also it exhibited a little higher kcat for
4 - nitrophenyl - beta - d - glucopyranoside (
PNPG ) and a noticeable higher hydrolysis efficiency towards
geniposide . -
pubmed
Other serum parameters including
alanine aminotransferase ,
aspartate aminotransferase ,
alkaline phosphatase , total
bilirubin , blood
urea nitrogen and
interleukin-6 were significantly higher in the overgrazing group . -
pubmed
Two - year CW - free survival was 81 % and 58 % , respectively (
HR CW
macitentan 2.16 [ 0.962 - 4.87 ] , p = 0.06 ) . -
pubmed
RAC - alpha serine / threonine - protein kinase (
AKT1 ) ,
caspase-3 (
CASP3 ) ,
caspase-8 (
CASP8 ) ,
mitogen - activated protein kinase 8 (
MAPK8 ) , and
mitogen - activated protein kinase 14 (
MAPK14 ) are the core targets regulated by
stemazole and play a central role in its anti - apoptosis effects . -
pubmed
AIM AND OBJECTIVE : In this work , water was used as solvent for the eco - friendly synthesis of
imines under microwave irradiation . -
pubmed
Active
vitamin D and
vitamin D analogs stimulate
fibroblast growth factor 23 production in osteocyte - like cells via the
vitamin D receptor . -
pubmed
Osteocytes play an important role in the regulation of serum
phosphorus by producing
fibroblast growth factor 23 (
FGF23 ) . -
pubmed
FGF23 production is stimulated by
1alpha,25 - dihydroxyvitamin D in osteocytes . -
pubmed
However , it is unclear whether
vitamin D induces
FGF23 production in osteocytes directly . -
pubmed
We also investigated differences in the induction of
FGF23 by
1alpha,25 - dihydroxyvitamin D and various
vitamin D analogs . -
pubmed
MCT3 -
E1 - OLCs were stimulated with
1alpha,25 - dihydroxyvitamin D3 and subsequent
FGF23 gene expression was 2631 + - 605 times higher compared with untreated cells . -
pubmed
Therefore , the induction of
FGF23 in osteocytes by
vitamin D may be primarily mediated via
VDR . -
pubmed
The potential of
25 ( OH ) vitamin D3 ,
paricalcitol , and
maxacalcitol to induce
FGF23 production was almost the same as that of
1alpha,25 - dihydroxyvitamin D3 . -
pubmed
However ,
falecalcitriol and
eldecalcitol demonstrated a reduced potential to induce
FGF23 compared with
1alpha,25 - dihydroxyvitamin D3 . -
pubmed
Our results demonstrate that
FGF23 induction is different among the analogs of
1alpha,25 - dihydroxyvitamin D3 . -
pubmed
Self - focusing
Au@
SiO2 nanorods with
rhodamine 6 G as highly sensitive
SERS substrate for
carcinoembryonic antigen detection . -
pubmed
A highly sensitive self - focusing surface - enhanced Raman scattering ( SERS ) methodology has been developed using
Au@
SiO2 core - shell nanorods for
carcinoembryonic antigen (
CEA ) detection . -
pubmed
The SERS enhancement factor was evaluated for anisotropic
Au@
SiO2 nanorods with
silica shells of various thicknesses , upon which
Rhodamine 6 G (
R6 G ) dye was applied as a reporter molecule for the quantitative determination of
CEA . -
pubmed
METHODS : In a large cohort study of the German Neonatal Network (
GNN ; n = 16 967 VLBWI ) we assessed the association of
vancomycin treatment and pathological hearing tests at discharge and at 5 year follow - up . -
pubmed
RESULTS : In the
GNN cohort , 28 % ( n = 4739 ) were exposed to
IV vancomycin therapy . -
pubmed
Reactive
oxygen species produced from PVAT can be originated from mitochondria ,
nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidases , and uncoupled
endothelial nitric oxide synthase . -
pubmed
Association Between Hypertriglyceridemic Waist Phenotype and Increased Urinary
Albumin -
Creatinine Ratio in Chinese Adults : The REACTION Study . -
pubmed
Additionally , strong evidence indicates that elevated urinary
albumin -
creatinine ratio ( UACR ) is associated with increased prevalence of
CVD . -
pubmed
Furthermore , the stratified analysis showed that the OR for high - risk significantly increased in individuals in the HTGW group aged below 65 years , with 5.6 < = fasting blood
glucose < 7.0 or 7.8 < =
post - load blood
glucose < 11.1 mmol / L , 120 < =
systolic blood pressure < 140 or 80 < = diastolic blood pressure < 90 , 24 < =
body mass index < 28 kg / m2 , and estimated globular filtration rate > 90 mL / min per 1.73 m2 . -
pubmed
FGF significantly inhibited
Abeta fibrillogenesis and disintegrated the mature fibrils as evidenced by
thioflavin T fluorescence and atomic force microscopy studies . -
pubmed
Molecular dynamics simulations further showed that
FGF could synergistically interact with the Abeta17 - 42 pentamer via electrostatic interactions ,
hydrogen bonds and pi - pi interactions , which reduced the beta - sheet content , and disordered random
coils and bend structures of the Abeta17 - 42 pentamer . -
pubmed
Functional enrichment analysis found several biological processes , including protein targeting to membrane , ribosome and negative regulation of programmed cell
death , were over - represented exclusively among up - regulated
DEGs in the resistant reaction , whereas significant enrichment of
salicylic acid (
SA ) and
jasmonic acid ( JA ) pathways observed for down - regulated
DEGs occurred only in the susceptible reaction . -
pubmed
In addition , a functionally - related
calcium channel , the
transient receptor potential vanilloid type 4 (
TRPV4 ) channel , has recently been linked to purinergic signaling and has also been shown to mediate lung IRI . -
pubmed
The nine analytes selected for the program were
amylase ,
calcium ,
phosphorus ,
sodium ,
potassium ,
creatinine , osmolality ,
17 - ketosteroids and
total protein . -
pubmed
Data obtained from more than 230 enrolled laboratories indicate deficiencies in assay performance for
amylase ,
17 - ketosteroids and
total protein as measured by consensus evaluation . -
pubmed
Of these isolates 12.6 % produced
beta - lactamase and were resistant to
ampicillin . -
pubmed
Haloperidol reduced the flash fusion threshold frequency at 6 h
post - dose . -
pubmed
Sulpiride prolonged the duration of the manipulative motor task , particularly at 48 h
post - dose . -
pubmed
Chlorpromazine significantly reduced the number of correct letter pair identifications at 2 , 4 and 6 h
post - dose ,
haloperidol at 4 , 6 , 8 , 24 and 48 h
post - dose , and
sulpiride at 24 h
post - dose. -
pubmed
The in vivo and in vitro oxidation of molecular form 1 of
biliverdin reductase to molecular form 3 by
diamide . -
pubmed
Although hepatic levels of
GSH also decreased , no mixed
disulfides of the
reductase and
GSH could be detected . -
pubmed
The
diamide also oxidized molecular form 1 of
biliverdin reductase in vitro and molecular form 3 was formed . -
pubmed
A competitive assay in which the labelled antigen was irradiated at 254 nm was found to be specific for
Pyr ( 6 - 4 ) Pyo adducts , the
antibody - binding sites being sensitive to a secondary photolytic dose of 320 - nm light . -
pubmed
On the horizon are
rimantadine and the
interferons . -
pubmed
When correlated with the known structure of the
HLA - B27 subtypes , these results demonstrate the critical relevance of
amino acid changes within residues 77 - 81 and at position 152 in modulating allospecific CTL recognition of
HLA - B27.1 and suggest that these residues could be involved in the structure of immunodominant regions of this
antigen . -
pubmed
The observed cross - reactions with
HLA - B40 , differing from B27.1 in 16
amino acid residues , suggest that the simultaneous occurrence of multiple
amino acid changes could have mutually compensatory effects , so that a cross - reactive epitope might result from various combinations of polymorphic residues . -
pubmed
Stimulation of postsynaptic
D2 - dopamine receptors by B -
HT 958 is revealed by co - treatment with the
D1 - receptor agonist
SKF 38393 . -
pubmed
The motor activity was only slightly stimulated by B -
HT 958 or by the
D1 - receptor agonist
SKF 38393 but it was markedly increased by the two drugs given in combination . -
pubmed
The enhanced motor activity was inhibited by the
D2 - receptor antagonist
sulpiride or the
D1 - receptor antagonist
SCH 23390 , indicating that it was caused by stimulation of both receptor types . -
pubmed
Isolated
light chain of tetanus toxin inhibits exocytosis : studies in
digitonin - permeabilized cells . -
pubmed
It has been concluded that the paradox response of the muscle membrane to changes in [ K+]o can be attributed to the K - dependent conductance changes of the
inward rectifier K channel providing an explanation for the plateau - formation of SMPO and for the existence of two stable levels of membrane potential of the skeletal muscle bathed in
Cl- -free medium . -
pubmed
Millisecond kinetics of
ATP synthesis driven by externally imposed electrochemical potentials in
chloroplasts . -
pubmed
We have used rapid mixing and quenching techniques to measure the initial
ATP synthesis rates and the duration of the
ATP synthetic capacity derived from artificially imposed proton gradients and
valinomycin - mediated K+ diffusion potentials in
chloroplasts . -
pubmed
The
serum alkaline phosphatase was significantly more accurate than total
bilirubin and SGOT in detecting
liver metastasis . -
pubmed
The sensitivity , specificity , and accuracy of the imaging studies in the presence of an abnormal
alkaline phosphatase ,
SGOT , or total
bilirubin were not significantly different than in the presence of normal biochemical tests . -
pubmed
Blood -
glucose estimations were performed on this sample by an automated
glucose - oxidase method and by a reflectance meter system ( Reflocheck , BCL ( London ) Ltd. , Lewes , East Sussex , UK ) . -
pubmed
Blood -
glucose values measured by the meter were found to be higher than those assayed by the
glucose - oxidase method by a mean ratio of 1.16:1 . -
pubmed
We conclude that hypertrophic chondrocytes respond directly to
ICF and CCF by an increased deposition of
calcium - phosphate mineral in the matrix . -
pubmed
Cirazoline produced a postsynaptic
alpha 1 - adrenoceptor mediated potentiation of the isometric contraction to single
pulse field stimulation in both prostatic and epididymal portions . -
pubmed
In epididymal portions ,
nifedipine ( 10 microM ) greatly attenuated the postsynaptic
alpha 1 - receptor mediated potentiation of nerve mediated contractions , uncovering a presynaptic inhibitory action of
cirazoline . -
pubmed
No evidence was found for
alpha 2 - antagonism by
cirazoline . -
pubmed
Glucose uptake was similar in both groups being 65.4 + /- 24.1 % of control in the
CS group and 73.9 + /- 29.8 % in the
AM group . -
pubmed
Fixation in
formol acetic acid was unsatisfactory for demonstration of
fibronectin ; prolonged fixation in
formol sublimate was unsatisfactory for demonstration of
laminin . -
pubmed
Trypsin was completely ineffective for unmasking
laminin antigens except after fixation in
ethanol acetic acid ; it was only partially effective for showing
fibronectin antigens . -
pubmed
Synthetic peptide
tyrosine phosphorylation was stimulated by
epidermal growth factor but not by
insulin or
relaxin . -
pubmed
The following components were tested :
T protein ,
M protein ,
opacity factor , lipoteichoic acid ,
hyaluronic acid ,
streptolysin S ,
streptolysin O ,
DNase ,
hyaluronidase and
NADase . -
pubmed
They increased the amount of
hyaluronic acid and
hyaluronidase , decreased the amount of
M protein , and enhanced phagocytosis and the release of lipoteichoic acid . -
pubmed
In contrast ,
scopolamine impaired working memory in both
MTS and NMTS tasks . -
pubmed
Individual
neomycin resistant colonies were screened for
alkaline phosphatase (
AP)-specific staining . -
pubmed
Both
AP activity and
osteocalcin (
OC ) secretion increased in a dose - dependent manner following
dihydroxyvitamin D3 (
1,25 - D3 ) treatment when cultured at either temperature . -
pubmed
Serum samples of autochthonous individuals from the Garrotxa prepyrenean region , and the Pallars Sobira valley were screened for
HP (
Haptoglobin ) and
ORM ( Orosomucoid ) types by
polyacrylamide isoelectric focusing and electrophoresis . -
pubmed
For immunostaining , the
avidin -
biotin complex technique was performed on paraffin sections of cell blocks , using a standardized automatic immunostainer . -
pubmed
Crystal structure at 1.9 - A resolution of
human immunodeficiency virus ( HIV ) II protease complexed with
L-735,524 , an orally bioavailable inhibitor of the
HIV proteases . -
pubmed
L-735,524 is a potent , orally bioavailable inhibitor of
human immunodeficiency virus ( HIV ) protease currently in a Phase
II clinical trial . -
pubmed
Both Fourier and difference Fourier maps reveal clearly the closure of the flap domains of the
protease upon
L-735,524 binding . -
pubmed
Specific interactions between the
enzyme and the inhibitor include the
hydroxy group of the
hydroxyaminopentane amide moiety of L-735,524 ligating to the
carboxyl groups of the essential
Asp-25 and
Asp-25 ' enzymic residues and the
amide oxygens of the inhibitor
hydrogen bonding to the backbone
amide nitrogen of
Ile-50 and
Ile-50 ' via an intervening water molecule . -
pubmed
Although other
hydrogen bonds also add to binding , an equally significant contribution to affinity arises from hydrophobic interactions between the
protease and the inhibitor throughout the pseudo - symmetric
S1 /
S1 ' ,
S2 /
S2 ' , and
S3 /
S3 ' regions of the
enzyme . -
pubmed
The mean ( + /- standard deviation ) rates of penetration into the cerebrospinal fluid (
CSF ) of infected animals were 6.7 + /- 3.9 % for
piperacillin given alone and 36.3 + /- 21.9 % for
tazobactam given alone . -
pubmed
The study shows that
tazobactam is able to provide effective protection against
piperacillin hydrolysis by the TEM-3 enzyme within the
CSF . -
pubmed
Appropriate dosage regimens of various
beta - lactam - tazobactam combinations may deserve comparative studies in experimental
meningitis caused by organisms producing extended - spectrum
beta - lactamases . -
pubmed
Biochemical analysis revealed normal values for
carnitines ,
carnitine palmityl transferase in serum and striated muscle , and beta - oxidation enzymes in striated muscles . -
pubmed
dose of
benzodiazepine ( BZD ) receptor ligand
methyl beta - carboline-3 - carboxylate (
beta - CCM ) , whether or not preceded by administration of two lower doses of
beta - CCM ( 0.5 and 1 mg / kg i.p . ) -
pubmed
In the other 4
strains , such injections induced either tolerance (
CPB - K , NZB ) , or sensitization ( C57BL/6J , XLII ) , whatever the compound subsequently administered (
beta - CCM or saline ) . -
pubmed
When
BMR is titrated with
NADPH or
sodium dithionite under anaerobic conditions , addition of 2 electron equivalents per mole of
BMR results in the reduction of the high potential
flavin (
FMN ) without the accumulation of
semiquinone intermediates . -
pubmed
Our results indicate that the kinetic and thermodynamic control of the
flavins in
BMR is significantly different from that in microsomal
P450 reductase . -
pubmed
The low potential of the anionic
FMN semiquinone can be utilized to reduce the
P450 heme . -
pubmed
When the anionic
semiquinone becomes protonated , its potential becomes more positive and it is readily reduced to
FMNH2 , which is not capable of reducing
P450 . -
pubmed
In the activation of adrenergic neurotransmission by a ganglionic nicotinic agonist , elevation of plasma
NPY - ir was also reduced by
propofol indicating the direct effect on peripheral adrenergic nerve terminals . -
pubmed
Plasma level of
NPY - ir reversed in parallel with the recovery of anaesthesia induced by
propofol . -
pubmed
Thus , a central nervous system effect of
propofol can also be considered in its effect on plasma
NPY - ir . -
pubmed
[ Combined therapy of
malignant tumors of the upper jaw and nasal cavity using pre- and
post - operative radiation with
metronidazol radiomodification ] . -
pubmed
These structures are of increasing interest since they may function as adhesion molecules ; adhesion of
tumour cells to endothelial cells of blood vessels may be mediated by an interaction between
sialosyl - Le ( x ) and
E - selectin and studies indicate that
Le ( y ) is related to cell motility . -
pubmed
The
TCA extract inhibited the activity of
type 1 protein phosphatase by 20 + /- 4 % ( n = 3 expts ) . -
pubmed
Dephosphorylation of the phosphorylated
TCA extract by
type 2 protein phosphatase reduced inhibition to 2 + /- 0.2 % ( n = 3 expts ) . -
pubmed
To determine whether
isoproterenol increases phosphorylation of
PPI-1 in cardiomyocytes , the
TCA extracts were prepared from cardiomyocytes treated with 1 microM
isoproterenol and from untreated cardiomyocytes . -
pubmed
With the use of a
rabbit skeletal muscle PPI-1 antibody , immunoblots of the
TCA extract of cardiomyocytes identified a 28 - kDa protein . -
pubmed
Hepatic
low density lipoprotein ( LDL ) receptor expression was not affected by
pravastatin throughout the experimental period . -
pubmed
Further analysis of
G protein cytoplasmic domain deletion mutants showed that a cytoplasmic domain of only 1
amino acid did not drive efficient budding , whereas 9
amino acids did . -
pubmed
Post - allo BMT bone loss correlated best with the cumulative
prednisolone dose at the LS and FN , and with average daily
prednisolone dose for TBBMC . -
pubmed
When the
rhG - CSF was secreted to extracellular broth in the presence of a non - ionic surfactant (
Tween 80 ) in the culture media , the multimerization of the secreted
rhG - CSF was efficiently prevented in the fed - batch cultures . -
pubmed
The
catecholamine - mediated modulation of the
cytokine network has primarily been demonstrated for leukocytes . -
pubmed
Whereas
catecholamines decrease the LPS - induced production of
IL-6 by leukocytes , serum levels of
IL-6 are dramatically increased by the
catecholamine epinephrine in animal
endotoxemia models . -
pubmed
Furthermore , these
catecholamines could even potentiate the LPS - induced
IL-6 protein production . -
pubmed
The
catecholamine - induced
IL-6 stimulation is based on increased IL-6 mRNA levels . -
pubmed
Thus , endothelial cells might be a possible source of increased
IL-6 production observed in situations such as stress or
septic shock , in which
catecholamines are elevated due to endogenous production or exogenous application . -
pubmed
Use of multivitamin supplements containing
folic acid diminished the adverse effects of
dihydrofolate reductase inhibitors , but not that of antiepileptic drugs . -
pubmed
The mechanisms involved in this selection process are not completely defined but appear to involve acquisition of
LH receptors on granulosa cells of the dominant follicle , increased
estradiol production by the dominant follicle , and inhibition of circulating
FSH concentrations . -
pubmed
At the time of selection of the dominant follicle , the normal increase in circulating
estradiol concentrations and subsequent
depression in circulating
FSH is blunted due to
estradiol metabolism . -
pubmed
In the case of the immune response to the
hapten (
4 - hydroxy-3 - nitrophenyl ) acetyl (
NP ) , as with several other designed immunogens , the process is dominated by a single key mutation , which greatly simplifies the modeling of and analysis of data . -
pubmed
CYP3A4 expression was assessed by Western blotting and by immunohistochemistry ; activity was measured by the rate of formation of
6beta - hydroxytestosterone from
testosterone . -
pubmed
Ondansetron : a selective
5 - HT ( 3 ) receptor antagonist and its applications in
CNS - related disorders . -
pubmed
Ondansetron is a selective
5 - hydroxytryptamine ( 3 ) ( 5 - HT ( 3 ) ) receptor antagonist that has been introduced to clinical practice as an antiemetic for
cancer treatment - induced and anesthesia - related
nausea and
vomiting . -
pubmed
OBJECTIVE : The aim of the present study was to investigate the relationship between the changes in lean and fat mass during
gonadotropin - releasing hormone agonist (
GnRH agonist ) therapy . -
pubmed
They were given
GnRH agonist (
leuprorelin acetate , 3.75 mg ) monthly for 4 months . -
pubmed
The present study analyzed using immunohistochemical labeling the distribution and co - localization of
nitric oxide synthase (
NOS ) ,
cocaine- and
amphetamine - regulated transcript peptide (
CARTp ) and
pituitary adenylate cyclase activating polypeptide (
PACAP ) with
choline acetyltransferase (
ChAT)-immunoreactive fibers in the guinea - pig stellate ganglia . -
pubmed
As
calcium and
phosphorus increased in the aortic valve , the mass ratios
Mg /
Ca and
Mg /
P decreased reversely in the aortic valve . -
pubmed
FS and
PME pretreatments produced a dose - dependent protection against
CCl 4 hepatotoxicity , with the effect of
FS being more potent . -
pubmed
The ensemble of results indicates that
FS /
PME produced a more potent in vivo antioxidant action than
alpha - Toc by virtue of their ability to enhance hepatic mitochondrial
glutathione antioxidant status and that the differential potency of
FS and
PME can be attributed to the difference in in vivo antioxidant potential between
Sch B and
emodin . -
pubmed
The addition of
tocopherol ,
ascorbate ,
prostaglandin E2 and
lipoxygenase inhibitors delayed cell
death , whereas the inhibition of
cyclooxygenase promoted AA - induced cell
death . -
pubmed
The mechanism did not involve blockage of the
interleukin-1 receptor , because
interleukin-12 did not suppress
interleukin-1 - mediated induction of
collagenase mRNA . -
pubmed
Here we describe a paralogue of
EBA-175 and show that this protein (
EBA-181 /
JESEBL ) binds in a
sialic acid - dependent manner to erythrocytes . -
pubmed
EM574 , a
motilide , has an orexigenic activity with affinity for
growth - hormone secretagogue receptor . -
pubmed
The most commonly employed humanized and chimeric MoAbs are
rituximab ,
alemtuzumab ( Campath-1H , Ilex Pharmaceuticals , San Antonio , TX ) , and gemtuzumab -
ozogamicin ( Mylotarg , Wyeth - Ayerst Laboratories , St Davids ,
PA ) . -
pubmed
Dihydrodipicolinate reductase (
DHPR ) catalyzes the reduced
pyridine nucleotide - dependent reduction of the
alpha , beta - unsaturated cyclic imine ,
dihydrodipicolinate , to generate
tetrahydrodipicolinate . -
pubmed
tuberculosis
DHPR uses
NADH and
NADPH with nearly equal efficiency based on V / K values . -
pubmed
Insulin sensitivity ( M - value ) was evaluated by the euglycaemic hyperinsulinaemic
glucose clamp technique . -
pubmed
The EHT - R had significantly higher levels of fasting
insulin and
triglyceride than did the
NT and the
EHT - N. The
EHT - R had higher levels of free
fatty acid and lower levels of
high - density lipoprotein cholesterol than did the
EHT - N. The M - value was negatively correlated with fasting
insulin ,
free fatty acid , and
triglyceride . -
pubmed
Approaches that have proven to be effective in animal models include the use of multi - epitope vaccines incorporating highly variable
amino terminal serotypic determinants , those based on the
carboxy terminal conserved region and combination vaccines incorporating both serotypic and conserved region determinants of the
M protein . -
pubmed
Standard REV of
phosphatidylcholine ( PC):
cholesterol (
CHOL ) in 7:3 molar ratio , and photoactive REV of PC :
CHOL :
SPF , and
DPPC :
CHOL :
SPF in 7:3:3 molar ratio were prepared . -
pubmed
DPPC led to a higher liposomal membrane stability ( lower
CAL release ) than PC . -
pubmed
Dietary intake and serum
calcium ,
magnesium ,
iron ,
retinol ,
alpha - tocopherol ,
albumin ,
cholesterol ,
glucose and hematological parameters were determined . -
pubmed
Mean serum
alpha - tocopherol /
cholesterol ratio in the whole population was
6.3 + /- 2.3 mmol / mol without gender differences . -
pubmed
Interaction of
aldehydes with
collagen : effect on thermal , enzymatic and conformational stability . -
pubmed
formaldehyde ,
gluteraldehyde ,
glyoxal and
crotanaldehyde was studied to understand the effect of each on the thermal , enzymatic and conformational stability of
collagen . -
pubmed
The variation in the thermal stability of
collagen brought about by
aldehydes was in the order of
formaldehyde & gt ;
gluteraldehyde & gt ;
glyoxal & gt ;
crotanaldehdye . -
pubmed
The
aldehydes also impart a high degree of stability to
collagen against the activity of the degrading enzyme ,
collagenase . -
pubmed
The effect of various
aldehydes on the secondary structure of
collagen was studied using circular dichroism and it was found that the
aldehydes lead to changes in the amplitude of the circular dichroic ( CD ) spectrum but did not alter the triple helical conformation of
collagen . -
pubmed
The secondary structure of
collagen is not significantly altered on interaction with different
aldehydes . -
pubmed
BACKGROUND : The associations of
proteinuria and an elevated
creatinine level with progression to
acquired immunodeficiency syndrome (
AIDS ) and
death in the
era of highly antiretroviral therapy ( HAART ) have not been fully described . -
pubmed
We suggest the
[ ( 99m ) Tc]
OKT3 scans may be useful for the monitoring of renal transplants to detect acute rejection . -
pubmed
These results show that
mGluR4 may play a role in locomotor activity in general and also display specificity for mediation of the motor stimulant effect of
ethanol . -
pubmed
Activation of
NF - kappaB signalling and
TNFalpha - expression in THP-1 macrophages by TiAlV- and
polyethylene - wear particles . -
pubmed
In this study we evaluated the effects of
polyethylene- and
TiAlV - particles on activation of
NF - kappaB signalling pathways and
TNFalpha biosynthesis and release in monocytic cells with respect to periprosthetic osteoclastogenesis . -
pubmed
A significant
TNFalpha release was detected after 360 min exposure to
polyethylene- and
TiAlV - particles in a dose dependent manner . -
pubmed
Muscle
cytokine mRNA changes after 2.5 h of cycling : influence of
carbohydrate . -
pubmed
Blood cell counts were determined , and plasma was analyzed for
interleukin ( IL )- 6 ,
IL-10 ,
IL-1 receptor antagonist ( ra ) ,
IL-8 ,
cortisol ,
epinephrine ,
glucose , and
insulin . -
pubmed
RESULTS : Plasma
glucose and
insulin were higher , and
epinephrine ,
cortisol ,
IL-6 ,
IL-10 , and
IL-1ra , but not
IL-8 , were significantly lower postexercise in CHO versus PLA . -
pubmed
The presence of five TAMs in the 41 , 215 pathway , at codons 41 , 67 , 210 , 215 , and 219 of
reverse transcriptase (
RT ) , produced a median 1.8 - fold reduction in SPD754 susceptibility , compared with fold reductions to
zidovudine ,
lamivudine ,
abacavir ,
didanosine and
tenofovir of 438 , 4.8 , 4.5 , 1.4 and 3.6 , respectively . -
pubmed
This 2 - part , double - blind , placebo - controlled study was conducted to determine the safety and efficacy of
etoricoxib , a
COX-2 selective inhibitor , for the treatment of
hemophilic arthropathy . -
pubmed
Studies have provided evidence that
HSL , though possessing
triacylglycerol lipase activity , appears to be the rate - limiting enzyme for
cholesteryl ester and
diacylglycerol hydrolysis in adipose tissue and is essential for complete hormone stimulated lipolysis , but other
triacylglycerol lipases are important in mediating
triacylglycerol hydrolysis in lipolysis . -
pubmed
HSL knockouts are resistant to both high fat diet - induced and genetic
obesity , displaying reduced quantities of white with increased amounts of brown adipose tissue , increased numbers of adipose macrophages , and have multiple alterations in the expression of genes involved in adipose differentiation , including transcription factors , markers of adipocyte differentiation , and enzymes of
fatty acid and
triglyceride synthesis . -
pubmed
Significant increases in mRNA expressions of some metabolism- and oxidative stress - related genes with a formation of
gamma - glutamyltranspeptidase (
GGT ) positive foci were observed in the
DMN + DC + PH group by the treatment of DC for 13 and 26 weeks . -
pubmed
RESULTS : There were significant differences in the age , male ,
alcohol - consuming , over - salt intake , average
systolic pressure , average
diastolic pressure , waist - to - hip - ratio , total
cholesterol ,
high - density lipoprotein , and
low density lipoprotein between the
hypertensive sib and the normotensive sib ( P & lt ; 0.05 ) . -
pubmed
Furthermore ,
IFN - gamma pretreatment of
NB cells increased
doxorubicin - induced apoptosis nearly 3 - fold . -
pubmed
Methylenetetrahydrofolate reductase (
MTHFR ) , a key regulatory enzyme in the metabolism of
folate , is suspected to play a role in the etiology of
cancer , via its effects on DNA methylation and
nucleotide synthesis . -
pubmed
One of 2 lines displayed a decrease in the number of osteoblasts , but in vivo
bromodeoxyuridine labeling demonstrated that
CHOP overexpression did not have an effect on osteoblastic cell replication . -
pubmed
BACKGROUND , AIMS AND SCOPE : In a previous study , we explored the use of
acetate ,
lactate , molasses ,
Hydrogen Release Compound (
HRC , which is based on a biodegradable
poly - lactate ester ) ,
methanol and
ethanol as
carbon source and electron donor to promote bacterial
sulfate reduction in batch experiments , this with regards to applying an in situ metal precipitation ( ISMP ) process as a remediation tool to treat heavy metal contaminated groundwater at the site of a nonferrous metal work company . -
pubmed
Therefore , the biological
sulfate reduction process in the column experiments was evaluated by combining classical analytical methods [ measuring heavy metal concentration ,
SO4 ( 2 -)-concentration , pH , dissolved organic
carbon ( DOC ) ] with molecular methods [ denaturing gradient gel electrophoresis ( DGGE ) fingerprinting and phylogenetic sequence analysis ] based on either the 16S rRNA - gene or the
dsr (
dissimilatory sulfite reductase ) gene , the latter being a specific biomarker for SRB . -
pubmed
Intentional disruption of the
lactate and
HRC supplies resulted in an immediate stagnation of the ISMP processes and in a rapid release of precipitated metals into the column effluents . -
pubmed
DGGE on the
dsrB genes was successfully used to link the results of the ISMP process to the community composition of the
sulfate reducing bacteria . -
pubmed
Uric acid ( UA ) ,
peroxidase and total antioxidant status ( TAS ) were studied in both saliva and serum ;
superoxide dismutase (
SOD ) was evaluated only in saliva . -
pubmed
RESULTS : Twenty one weeks after
SAS : mass index ( SG - HF>SG>CG , p<0.05 ) ;
systolic pressure ( SG - HF= SG>CG , p<0.05 ) ;
diastolic pressure ( SG - HF>SG>CG , p<0.05 ) ; systolic and diastolic meridional stress ( SG - HF>SG>CG , p<0.05 ) ; LV myocyte cross - sectional area ( SG - HF>SG>CG , p<0.05 ) and
hydroxyproline content ( SG - HF>SG>CG , p<0.05 ) . -
pubmed
DEVELOPMENT : Clinical trials that study the use of
alteplase in the
acute stroke treatment , especially the two phase III clinical trials carried out by the National Institute of
Neurological Disorders and
Stroke rt -
PA Stroke Study Group , are described . -
pubmed
Prior studies indicate that
hLa is activated by phosphorylation of
serine-366 by protein kinase
CK2 , neutralizing a negative effect of a short basic motif ( SBM ) . -
pubmed
These mutational oncogenic differences could lead to different therapeutic responses : nonmucinous BAC has been found to be sensitive to
EGFR tyrosine kinase inhibitors , while
mucinous BAC might be more responsive to
taxane - based chemotherapy . -
pubmed
Apoptosis was detected by flow cytometry , using
annexin V and by cytological detection with
fluorescein diacetate /
propidium iodide staining . -
pubmed
Without using density - gradient centrifugation , the bone marrow aspirate was poured into a dish ( 6.0 \times 10(5 ) nucleated cells / cm(2 ) ) with DMEM medium containing 10 % serum ( FCS or donor serum ) and
basic fibroblast growth factor , and incubated at 37 degrees C under a 5 %
CO ( 2 ) atmosphere . -
pubmed
Herein , we have explored the role of 3 key components (
Apc ,
beta - catenin , and
c - Myc ) of the Wnt pathway in the zonation of
ammonia metabolizing enzymes . -
pubmed
RESULTS : Under normal circumstances , the
urea cycle enzyme
carbamoylphosphate synthetase I (
CPS I ) is present in the periportal , intermediate , and the first few layers of the perivenous zone . -
pubmed
Bile acids inhibit
duodenal secretin expression via
orphan nuclear receptor small heterodimer partner (
SHP ) . -
pubmed
Small heterodimer partner (
SHP ) is an
orphan nuclear receptor in which gene expression can be upregulated by
bile acids . -
pubmed
Here , we evaluated the regulation on duodenal
secretin gene expression by
SHP and selected
bile acids ,
cholic acid ( CA ) and
chenodeoxycholic acid (
CDCA ) . -
pubmed
In vitro treatment of
CDCA or
fexaramine elevated the
SHP transcript level and occupancy on
secretin promoter . -
pubmed
The increase in the
SHP level , induced by
bile acid treatment or overexpression , reduced
secretin gene expression , whereas this gene inhibitory effect was reversed by silencing of endogenous
SHP . -
pubmed
A diet enriched with 5 %
cholestyramine led to a decrease in
SHP level and a corresponding increase in
secretin expression . -
pubmed
Overall , this study showed that
bile acids via
SHP inhibit duodenal
secretin gene expression . -
pubmed
Because
secretin is a key hormone that stimulates bile flow in cholangiocytes , this pathway thus provides a novel means to modulate
secretin - stimulated choleresis in response to intraduodenal
bile acids . -
pubmed
Role of
phosphatidylinositol mannosides in the interaction between mycobacteria and
DC - SIGN . -
pubmed
Recently , we have shown that although the
mannose caps of the mycobacterial surface glycolipid lipoarabinomannan ( ManLAM ) are essential for the binding to
DC - SIGN , genetic removal of these caps did not diminish the interaction of whole mycobacteria with
DC - SIGN and DCs . -
pubmed
Here we investigated the role of the structurally related glycolipids
phosphatidylinositol mannosides (
PIMs ) as possible ligands for
DC - SIGN . -
pubmed
In a binding assay with both synthetic and natural PIMs ,
DC - SIGN exhibited a high affinity for hexamannosylated PIM(6 ) , which contains terminal alpha(1 - ->2)-linked
mannosyl residues identical to the
mannose cap on
ManLAM , but not for di- and tetramannosylated PIM(2 ) and PIM(4 ) , respectively . -
pubmed
bovis bacillus Calmette - Guerin deficient in the production of
PIM(6 ) ( Delta pimE ) was created , as well as a double knockout deficient in the production of both PIM(6 ) and the
mannose caps on
LAM ( Delta pimE Delta
capA ) . -
pubmed
The nematode eukaryotic
translation initiation factor 4E / G complex works with a trans - spliced leader stem - loop to enable efficient translation of
trimethylguanosine - capped RNAs . -
pubmed
Heme oxygenase-1 (
HO-1 ) , an inducible enzyme , degrades
heme to
carbon monoxide (
CO ) ,
iron , and
bilirubin . -
pubmed
HO enzyme activity was reduced immediately following
CO exposure , while expression of
HO-1 protein was consistently upregulated in a time - dependent manner . -
pubmed
We suggest that
CO initially inhibits
HO enzyme activity , whereas at later time points the inhibition is released and local
CO generation is maintained by the activity of the endogenous
HO enzyme . -
pubmed
And the noninducible form of
heme oxygenase ,
HO-2 , was not altered following
CO administration . -
pubmed
Patterns of
iodine intake and urinary
iodine concentrations during pregnancy and blood
thyroid - stimulating hormone concentrations in the newborn progeny . -
pubmed
Iodine nutrition was assessed by measuring urinary
iodine concentration ( UIC ) and neonatal
thyrotropin (
TSH ) . -
pubmed
Plasma determinations included
TSH , free
thyroxine ,
thyroid peroxidase antibody , and
thyroglobulin antibody . -
pubmed
In addition , neonate
TSH screening is not the best tool to assess whether the
iodine status in populations is ideal . -
pubmed
Maternal blood was collected at both necropsies and at weekly intervals for assay of
glucose ,
insulin and
leptin . -
pubmed
Improvement in
metformin and
insulin utilisation in the Australian veteran population associated with quality use of medicines intervention programs . -
pubmed
PURPOSE : To examine the impact of 2001 and 2005 quality use of medicines ( QUMs )
diabetes programs implemented by National Prescribing Service (
NPS ) on the prevalence of utilisation of
metformin and
insulin among the Australian
diabetes veteran population . -
pubmed
Prevalence of utilisation of
metformin and
insulin were assessed . -
pubmed
RESULTS : Of the
diabetes population , over 55 % has been dispensed
metformin , and around 20 %
insulin . -
pubmed
Twenty - four months post the onset of second
NPS diabetes intervention , there was 4.2 % relative increase in
metformin use , and 13.5 % relative increase in
insulin used concurrently with oral hypoglycaemics , compared to the estimates without the interventions . -
pubmed
Density functional calculations were carried out to ascertain the origin of enantioselectivity in the
brucine N - oxide (
BNO)-assisted enantioselective Pauson - Khand reaction (
PKR ) of
norbornene with
2 - methyl-3 - butyn-2 - ol . -
pubmed
Delaying
ART initiation by at least 2 weeks after
amphotericin B treatment may decrease the incidence of
IRIS . -
pubmed
Rst2 and
Ste11 proteins were induced and localized to the nucleus of
sam5 and
sam7 mutants even under rich
glucose conditions , indicating that the function of
Pka1 was completely abolished by mutations . -
pubmed
Pka1 - G441E and
Pka1 - G441R mutant proteins reside in the cytoplasm , even under
glucose - rich conditions , while wild - type
Pka1 resides in the nucleus , indicating that the functionality of
Pka1 is important for its nuclear localization . -
pubmed
This is supported by the observation that the
Pka1 - T356A mutant , which partially lacks
Pka1 function , was localized to both the cytoplasm and the nucleus , but an active phosphomimetic
Pka1 - T356D mutant
prtotein was localized to the nucleus under
glucose - rich conditions . -
pubmed
In addition to the basal phosphorylation of
Pka1 at T356 , hyperphosphorylation of
Pka1 was observed under
glucose - starved conditions , and such hyperphosphorylation was not observed in
pka1 - G441E ,
pka1 - G441R ,
pka1 - T356A or
pka1 - T356D mutants . -
pubmed
Multiple cells of Deltaproteobacteria cluster SAR324 , Gammaproteobacteria clusters ARCTIC96BD-19 and Agg47 , and some Oceanospirillales from the lower mesopelagic contained
ribulose-1,5 - bisphosphate carboxylase - oxygenase and
sulfur oxidation genes . -
pubmed
This research employed
( 29 ) Si and
( 13 ) C Cross - Polarisation / Magic Angle Spinning (
CP / MAS ) NMR spectroscopy to characterise the nature and amount of surface species of
di - and trifunctional mercaptopropylsilane (
MPS ) bonded
silica using supercritical
carbon dioxide ( sc -
CO ( 2 ) ) as a reaction solvent without additives ( co - solvent ) or catalysts . -
pubmed
Sirius red staining was employed to detect the expression of myocardial
collagen , and the myocardial
collagen volume fraction ( CVF ) and perivascular
collagen volume area ( PVCA ) were calculated . -
pubmed
In the
DOC group and
ALI group , the CVF and PVCA were significantly increased ; the RA and
Ang II levels in the plasma and ventricle were remarkably lowered when compared with the CON group . -
pubmed
The RA and
Ang II levels in the ventricle of the ALI group were significantly lower than those in the
DOC group . -
pubmed
Moreover , the expressions of
ERK1 / 2 ,
PERK1 / 2 and
MMP9 were the lowest in the CON group , but those in the ALI group were significantly reduced as compared to the
DOC group . -
pubmed
ALI can inhibit the
DOCA induced
myocardial fibrosis independent of its pressure - lowing effect , which may be related to the suppression of RA and
Ang II production , inhibition of
ERK1 / 2 phosphorylation and
MMP9 expression in the heart . -
pubmed
Approved drugs include
tyrosine kinase inhibitors ( TKI ) such as
sunitinib ,
sorafenib and
pazopanib ,
vascular endothelial growth factor inhibitors such as
bevacizumab , and
mammalian target of rapamycin (
mTOR ) inhibitors such as
temsirolimus and
everolimus . -
pubmed
Pharmacological inhibition of
GSK-3 by
SB216763 dose - dependently attenuated
TGF - beta1 - induced expression of these myofibroblasts markers . -
pubmed
Moreover , silencing of
GSK-3 by siRNA or pharmacological inhibition by
CT /
CHIR99021 fully inhibited the
TGF - beta1 - induced expression of
alpha - sm - actin and
fibronectin . -
pubmed
Neither
smad ,
NF - kappaB nor
ERK1 / 2 were involved in the inhibitory actions of
GSK-3 inhibition by
SB126763 on myofibroblast differentiation . -
pubmed
Rather ,
SB216763 increased the phosphorylation of
CREB , which in its phosphorylated form acts as a functional antagonist of
TGF - beta /
smad signalling . -
pubmed
OBJECTIVES :
Hepcidin , a peptide hormone released by hepatocytes into circulation is the main regulator of dietary
iron absorption and cellular
iron release . -
pubmed
The effects of ( -)-
noradrenaline , mediated through
beta1 adrenoceptors (
beta2 adrenoceptors blocked with
ICI118551 ) , and ( -)-
adrenaline , mediated through
beta2 adrenoceptors (
beta1 adrenoceptors blocked with
CGP20712A ) , were assessed in the absence and presence of
PDE inhibitors . -
pubmed
Concurrent therapy with a
PDE3 blocker and
metoprolol could conceivably facilitate cardiostimulation evoked by
adrenaline through
beta2 adrenoceptors . -
pubmed
RESULTS : Both the doses of
vanillin reduced the immobility duration in
TST as well as in
FST . -
pubmed
But the reduction of immobility in
FST did not show statistically significant reduction in immobility in the groups treated with
vanillin when compared with control . -
pubmed
To further study the functional process of
DHS regulated bone metabolism , the objective of this study was to evaluate the effects of
DHS on cortical bone and its alterations on gene expressions of osteogenic growth factors and transcription factors as a function of time . -
pubmed
A lincRNA -
DYNLRB2 - 2 /
GPR119 /
GLP-1R /
ABCA1 - dependent signal transduction pathway is essential for the regulation of
cholesterol homeostasis . -
pubmed
Accumulated evidence shows that
G protein - coupled receptor 119 (
GPR119 ) plays a key role in
glucose and lipid metabolism . -
pubmed
In hd - PS matched cohorts , the overall
MI rate difference (
RD ) comparing
prasugrel with
clopidogrel was -23.1 ( 95 % confidence interval [ CI ] -62.8 - 16.7 ) events per 1,000 person - years and RDs were -0.5 ( -12.9 - 11.9 ) and -2.8 ( -13.2 - 7.6 ) for a composite
bleed event outcome and
death from any cause , respectively . -
pubmed
RATIONALE :
Dihydropyridines are widely used for the treatment of several
cardiac diseases due to their blocking activity on
L - type Ca ( 2 + ) channels and their renowned antioxidant properties . -
pubmed
METHODS : We synthesized six novel
dihydropyridine molecules and performed docking studies on the binding site of the
L - type Ca ( 2 + ) channel . -
pubmed
The
Ca ( 2 + ) channel - blocking activity was evaluated by confocal microscopy on
fluo-3AM loaded cardiomyocytes , as well as using patch
clamp experiments . -
pubmed
RESULTS : Our docking studies show that a novel compound with
3 - OH substitution inserts into the active binding site of the
L - type Ca ( 2 + ) channel previously described for
nitrendipine . -
pubmed
In biochemical assays , the novel
meta - OH group in the
aryl in C4 showed a high blocking effect on
L - type Ca ( 2 + ) channel as opposed to para - substituted compounds . -
pubmed
CONCLUSIONS : Only substitutions in
C2 , C3 and
C5 of the
aryl ring render
dihydropyridine compounds with the capacity of blocking
LTCC . -
pubmed
Based on our docking studies , we postulate that the antioxidant activity requires a larger group than the
meta - OH substitution in
C2 ,
C3 or
C5 of the
dihydropyridine ring . -
pubmed
In the current study , we measured the correlations among physical activity , nutrient intake , smoking , body - mass index (
BMI ) , and age with the concentration of
triglycerides ,
cholesterol ,
phospholipids , and
apolipoproteins (
ApoA1 ,
ApoA2 and
ApoB ) in subfractions of
LDL and
HDL in 265 healthy working men . -
pubmed
Concentrations of
cholesterol , phospholipids , and
ApoB in small , dense atherogenic
LDL particles (
sdLDL ) correlated negatively ( p<0.001 ) with those of
cholesterol ,
phospholipids , and
ApoA1 in
HDL2 , respectively . -
pubmed
Age correlated positively with
sdLDL while increasing
BMI correlated with an atherogenic shift of
cholesterol ,
phospholipids , and
ApoB from large , buoyant
LDL (
lbLDL ) to
sdLDL and decreasing concentrations of
HDL2 constituents . -
pubmed
Physical activity and
alcohol intake correlated negatively with
sdLDL constituents and positively with
HDL2 components . -
pubmed
Consumption of
monounsaturated fatty acids (
MUFA ) correlated with a lower ratio of
sdLDL to
HDL2 cholesterol . -
pubmed
A multivariate model based on the product age - by - MELD ( OR = 0.81 ; P = 0.013 ) , delta eosinophils ( OR = 1.5 ; P = 0.002 ) and mean
tacrolimus trough concentrations & lt;6 ng / ml within the prior 4 days ( OR = 11.4 ; P = 0.047 ) had an AUROC = 0.84 to diagnose moderate - severe histological
ACR . -
pubmed
Long - Term Effects of Novel Combination Coating Anti -
CD34 Antibody Applied on
Sirolimus - Eluting Stents . -
pubmed
Moreover , we showed that
propyl pyrazole triol ( an
ERalpha agonist ) stimulates the
phosphatidyl inositol 3 - kinase (
PI3 K ) pathway specifically in
POMC progenitor neurons , and that blockade of
PI3 K attenuates
propyl pyrazole triol - induced activation of POMC neurons . -
pubmed
From the morula stage onward , in vitro fertilized embryos were cultured in the presence of cell - permeable small molecules targeting nine principal signaling pathway components , including
TGFbeta1 ,
BMP ,
EGF ,
VEGF ,
PDGF ,
FGF ,
cAMP ,
PI3 K , and
JAK signals . -
pubmed
TGFbeta1 ,
BMP , and
cAMP and combined
VEGF /
PDGF / FGF signals did not affect blastocyst development while
PI3 K was important for ICM growth but did not alter lineage - specific gene expression . -
pubmed
Stimulating
cAMP specifically increased
NANOG expression , while combined
VEGF /
PDGF /
FGF inhibition up - regulated epiblast and hypoblast markers . -
pubmed
The strongest effects were observed by suppressing
JAK1 / 2 signaling with
AZD1480 . -
pubmed
We found that
tyrosine ( Y ) 705 - phosphorylated
STAT3 ( pSTAT3(Y705 ) ) was restricted to ICM nuclei , where it colocalized with
SOX2 and
NANOG . -
pubmed
The concentration - dependent internalization / bio - uptake of
Cr2O3 NPs may have contributed to the enhanced production of anti oxidant enzyme ,
superoxide dismutase to counteract the oxidative stress , which in turn resulted in observed
chromosomal aberrations and cytogenetic effects . -
pubmed
To produce red pigments , birds ingest yellow
carotenoids and endogenously convert them into red
ketocarotenoids via an oxidation reaction catalyzed by a previously unknown
ketolase [ 4 - 6 ] . -
pubmed
Lastly , we present evidence that
CYP2J19 is involved in
ketocarotenoid formation in the retina . -
pubmed
Without
betamethasone , PEEP increased FRC recruitment and
CDL Surfactant did not further increase FRC , but significantly increased
CDL Betamethasone abolished the benefit of PEEP on FRC , but surfactant counteracted this effect of
betamethasone . -
pubmed
Procaine also inhibited
JAK2 and
STAT3 expression in both mRNA ( p<0.05 ) and protein levels . -
pubmed
Overexpression of
JAK2 increased
STAT3 level and reversed the improvement effects of
procaine in
pain behaviors ( p<0.01 ) . -
pubmed
OBJECTIVE : The
toxicity of anti -
cancer chemotherapeutic agents can be reduced by associating these compounds , such as the anti - proliferative agent
paclitaxel , with a
cholesterol - rich nanoemulsion ( LDE ) that mimics the lipid composition of
low - density lipoprotein (
LDL ) . -
pubmed
Familial Hyperaldosteronism type 1 (
FH1 ) or
dexamethasone suppressible
hyperaldosteronism , FH2 , the most common form of as yet unknown cause(s ) , and
FH3 . -
pubmed
FH3 is due to activating mutations of the
potassium channel gene
KCNJ5 that increase constitutive and
angiotensin II - induced
aldosterone synthesis . -
pubmed
The
enzyme was purified to homogeneity by
ammonium sulfate precipitation , Sephadex G-75 gel filtration ,
Mono - Q anion - exchange chromatography and ultrafiltration , with a 12.70 - fold increase in specific activity and 20.29 % recovery . -
pubmed
In
photosystem II (
PSII ) , water oxidation occurs at a
Mn4CaO5 cluster and results in production of molecular
oxygen . -
pubmed
The calculated frequencies were compared to
S2 - minus -
S1 reaction - induced Fourier transform infrared spectra acquired from
chloride- ,
bromide- , or
nitrate - containing PSII samples , which were active in
oxygen evolution . -
pubmed
These results support the conclusion that an internal
hydronium ion and
chloride play a direct role in an internal proton transfer event during the
S1 - to - S2 transition . -
pubmed
Phase -
II sulfate conjugation of
phenolic compounds is generally thought to result in their inactivation . -
pubmed
Calixarene - Based
Copper ( I ) Complexes as Models for
Monocopper Sites in
Enzymes . -
pubmed
When substoichiometric rhombohedral
alpha - SrUO4 - x , in space group R3 m with disordered in - plane
oxygen defects , is heated above 200 C in a
hydrogen atmosphere it undergoes a first order phase transformation to a ( disordered ) triclinic polymorph ,
delta - SrUO4 - x , in space group
P1 . -
pubmed
Although CCK8 and colony - formation assays showed that
SIRT2 overexpression marginally promoted proliferation in
GC cell lines ,
SIRT2 knockdown or treatment with
SirReal2 decreased the migration and invasion of
GC cells . -
pubmed
We demonstrated both in vitro and in vivo that
SirReal2 could inhibit the deacetylation activity of
SIRT2 and its downstream target
PEPCK1 , which is related to mitochondrial metabolism and
RAS /
ERK /
JNK /
MMP-9 pathway . -
pubmed
SirReal2 therefore shows promise as a new drug candidate for
GC therapy . -
pubmed
In particular , the transition pore opening induced by
AGE was due to the rearrangement of the mitochondrial membranes following the increased activity of the
K +/ H + exchanger . -
pubmed
Pab1 consists of four RNA recognition motifs ( RRM ) , a
proline - rich domain ( P ) and a
carboxy - terminal ( C ) domain . -
pubmed
However ,
hyperoxia decreased cardiac tissue three -
nitrotyrosine formation ( P & lt ; 0.001 ) and
inducible nitric oxide synthase expression ( P = 0.016 ) . -
pubmed
Gemcitabine plus S-1 (
GS ) is commonly used to treat advanced
pancreatic cancer (
APC ) in Asia . -
pubmed
Here we demonstrated forty - seven filamentous fungal isolates were obtained from eight soil samples using a new screening strategy with both
triphenyltetrazolium chloride (
TTC ) , a redox indicator used for testing oil presence , and
cerulenin , an inhibitor of
fatty acid synthase (
FAS ) , supplemented in screening medium . -
pubmed
1 ) We found that the administration of recombinant
fetuin B aggravated hepatic lipid accumulation caused by
free fatty acids ( FFAs ) or high fat diet , in vivo and in vitro . -
pubmed
It lowered the phosphorylated
AMPK levels and activated the
LXR -
SREBP1c pathway , accompanied by the downregulation of
fatty acid oxidation and upregulation of lipogenesis . -
pubmed
It enhanced phosphorylated
AMPK and depressed the
LXR -
SREBP1c pathway , accompanied by upregulation of
fatty acid oxidation and downregulation of lipogenesis . -
pubmed
On one hand ,
mycolactone induces analgesia by targeting
type-2 angiotensin II receptors (
AT2R ) , causing cellular hyperpolarization and neuron desensitization . -
pubmed
Her
serum creatine kinase was 6,300 U / L.
Pitavastatin was stopped , but her symptoms deteriorated , and
cardiac congestion appeared . -
pubmed
Three SNPs caused
amino acid changes in
NAD - ME1 in CSR12906 , N299S , S488N and S544N. -
pubmed
Ribavirin plasma trough concentrations were determined at week 4 of therapy using a liquid chromatography / tandem mass spectrometry ( LC -
MS /
MS ) . -
pubmed
For clinical outcomes , sustained virological response ( SVR ) , liver function tests (
ALT and
AST ) , total
bilirubin ,
albumin , serum
creatinine ,
hemoglobin , leukocyte count , and platelet count were measured . -
pubmed
RESULTS : Concerning
RBV pharmacokinetics ,
ABCB1 2677 G > T SNP and
ABCB11 1331 T > C SNP were statistically associated with RBV Ctrough levels after 4 weeks of therapy . -
pubmed
SLC28A2 - 146 A > T SNP has not showed any statistically significant association with
RBV plasma levels or response . -
pubmed
CONCLUSION : SNP genotyping for
ABCB1 and
ABCB11 genes can help in better personalized medicine for maximizing response for
ribavirin as explored by the significant association between polymorphism in
ABCB1 and
ABCB11 genes and
ribavirin pharmacokinetics and the significant association of
ABCB11 1331 T > C SNP with clinical response . -
pubmed
The aim of this study was to examine whether combining a novel
BRD4 inhibitor ,
ITH-47 , with the antimitotic
estradiol analogue ,
ESE-15 - ol , would have a synergistic effect on inhibiting the growth of two different
breast cancer cell lines in vitro . -
pubmed
Our docking and molecular dynamics studies showed that compared to
JQ1 ,
ITH-47 showed a similar binding mode with
hydrogen bonds forming between the ligand
nitrogens of the
pyrazole ,
ASN99 , and water of the
BRD4 protein . -
pubmed
Of these pendant - bearing GNGs ( P - GNGs ) , three detergents (
GNG-2,14 ,
GNG-3,13 and
GNG-3,14 ) were not only notably better than both
DDM ( a gold standard detergent ) and the previously described GNGs at stabilizing all six membrane proteins tested here , but were also as efficient as
DDM at membrane
protein extraction . -
pubmed
The regression model showing an acceptable fit to the experimental data ( adjusted
R2 0.84 ) for
AFB1 as a function of grains steeping degree , temperature and time of germination . -
pubmed
An
amino - terminal point mutation increases
EAAT2 anion currents without affecting
glutamate transport rates . -
pubmed
Excitatory
amino acid transporters (
EAATs ) are prototypic dual function proteins that function as coupled
glutamate /
Na+/H
+/K+ transporters and as anion - selective channels . -
pubmed
In contrast , changes in selectivity made
gluconate permeant in L46P
EAAT2 , and non - stationary noise analysis revealed slightly increased unitary current amplitudes in mutant
EAAT2 anion channels . -
pubmed
We used unitary current amplitudes and individual transport rates to quantify absolute open probabilities of
EAAT2 anion channels from ratios of anion currents by
glutamate uptake currents . -
pubmed
In the present study , we evaluated four small molecule affinity - based probes based on
agarose - immobilized
benzamidine (
ABA ) ,
O - Phospho - L - Tyrosine (
pTYR ) ,
8 - Amino - hexyl - cAMP (
cAMP ) , or
8 - Amino - hexyl - ATP (
ATP ) for their ability to remove high - abundant proteins such as
serum albumin from plasma samples thereby enabling the detection of medium - to - low abundant proteins in plasma samples by mass spectrometry - based proteomics . -
pubmed
To clarify whether
PXR and
CAR regulate various genes involved in drug metabolism of high - altitude
hypoxia , we investigated the expression of
CYP1A2 ,
CYP2C9 ,
CYP2E1 ,
CYP3A4 , and
UGT1A1 using a dual -
luciferase reporter assay after treatment with
Ketoconazole (
KCZ ) and
Retinoic acid ( RA ) , or silenced
PXR and
CAR gene expression . -
pubmed
Activation of
PXR and
CAR in cells treated with
6 -( 4 - chlorophenyl )- imidazo ( 2,1 - b ) thiazole-5 - carbaldehyde (
CITCO ) and
rifampicin (
Rif ) resulted in the enhancement of
CYP450 isoforms ,
UGT1A1 ,
PXR , and
CAR . -
pubmed
The level of membrane
phosphatidylserine , a target of the
scavenger receptors , which initiates removal of damaged cells and modified lipoproteins from the circulation was also elevated . -
pubmed
BACKGROUND : Prophylactic pancreatic stent placement (
PSP ) and rectal
indomethacin suppository are recommended to prevent
post - endoscopic retrograde cholangiopancreatography ( ERCP )
pancreatitis (
PEP ) in high - risk cases . -
pubmed
Clinical trials on the use of
nitroglycerin to reduce
PEP have reached no definitive conclusion . -
pubmed
The three treatment groups were : the placebo group , the
indomethacin +
nitroglycerin group , and the
PSP group . -
pubmed
The rate of
PEP in the three study groups placebo group ,
indomethacin +
nitroglycerin group and the
PSP group was 19.3 % , 5.1 % , and 12.1 % , respectively . -
pubmed
CONCLUSIONS : The risk of
post - ERCP
pancreatitis in the
indomethacin +
nitroglycerin group was 7 % lower than that in the
PSP . -
pubmed
Indomethacin plus
nitroglycerin can avoid the need for
PSP in the prevention of
post - ERCP
pancreatitis . -
pubmed
Growth in
methanol - containing medium results in dramatic induction of genes in the
alcohol oxidation pathway including
alcohol oxidase (
AOX ) ,
formaldehyde dehydrogenase (
FLD ) , and
dihydroxyacetone synthase (
DHAS ) . -
pubmed
17beta - Estradiol (
E2 ) confers neuroprotection in preclinical models of
spinal cord injury when administered systemically . -
pubmed
The goal of this study was to apply
E2 locally to the injured spinal cord for a sustained duration using
poly ( pro - E2 ) film biomaterials . -
pubmed
To increase the
E2 dose released , bolus - releasing
poly ( pro - E2 ) films were fabricated by incorporating unbound
E2 into the
poly ( pro - E2 ) films . -
pubmed
However , compared to the injured controls , bolus - releasing
poly ( pro - E2 ) films did not significantly enhance neuroprotection or limit
inflammation at either 7 or 21 days
post -
injury . -
pubmed
The fluorescent peptide substrate
KKRAARAC(
sulfobenzo - furazan ) SNVFS - amide was used to measure
kinase activity . -
pubmed
We were able to remove the contaminating antibody from the anti -
calmodulin by passing the affinity - purified mixture over an
antigen - free Affi - Prep 10 column . -
pubmed
The antipruritic effect of 3 days of treatment with the non - sedative
H1 antagonist
terfenadine ( 60 mg b.i.d . ) -
pubmed
Amiloride inhibits the
protein tyrosine kinases associated with the cellular and the transforming
src - gene products . -
pubmed
In immunocomplexes ,
TBR - IgG serves as an artificial but specific
phosphate acceptor . -
pubmed
The
phosphate incorporation into
TBR - IgG is a time- and temperature - dependent process . -
pubmed
In the presence of
amiloride the TBR -
IgG phosphorylation is reduced . -
pubmed
Half - maximal inhibition of the
tyrosine kinase occurs at 300 microM
amiloride and is competitive with respect to
ATP . -
pubmed
Viral pp60src kinase of transformed cells is more sensitive to
amiloride ( IC50 : 50 - 100 microM ) . -
pubmed
Furthermore , normal cellular
tyrosine kinases are to a lesser extent inhibited by
amiloride as compared to the transforming viral
pp60src kinase . -
pubmed
These results may indicate different
amiloride - sensitive forms of cellular
pp60src kinases . -
pubmed
Furthermore ,
MAL transplantation is associated with less increased levels of plasma
ammonia during
acute liver ischemia . -
pubmed
The compound
CY 222 was administered as 1.4 mg / kg ( group A ) by
IV injection immediately after start of
t - PA infusion ( 0.125 and 0.250 mg / kg ) and then hourly during the 4 hours of infusion of the thrombolytic ; results were compared with those obtained in the absence of
CY 222 ( group B ) . -
pubmed
The conclusion is reached of the interest of
CY 222 in combination with
t - PA , the
CY 222 acting not only on coagulation by also on
thrombolysis by potentiating the effect of
t - PA . -
pubmed
All alpha subunits bind
guanine nucleotides and are
ADP - ribosylated by either
pertussis toxin (
Gi ,
transducin ,
Go ) or
cholera toxin (
Gs ,
Gi ,
transducin ) . -
pubmed
Thus ,
transducin and
Gi , which have the highest degree of sequence homology , are also
ADP - ribosylated by both toxins . -
pubmed
While the gamma subunits of
Gs , Gi , and Go have identical electrophoretic mobility in
SDS gels , they differ significantly in this respect from the gamma subunit of
transducin . -
pubmed
[
Auranofin and
aurothiomalate sodium : a comparative review ( and
II ) Efficacy , tolerance and safety ] . -
pubmed
Eight responders were monitored for
F - AT throughout
cyclophosphamide chemotherapy . -
pubmed
Failure to accumulate protein
methyl esters , either in vitro in in vivo , was most likely due to the presence of a very active
protein methylesterase . -
pubmed
The turnover of
protein methyl esters was primarily enzymatic , since no
[ 3H ] methanol was formed when
ACTH was incubated with
AdoMet and purified
protein carboxyl methyltransferase . -
pubmed
Utilizing
[ 3H ] methanol formation as an index of the rate of protein methylation - demethylation would yield minimal values , since this compound was oxidized via
alcohol dehydrogenase or
oxidase in these cells . -
pubmed
The isolated
homoaconitic acid was also identified as a substrate for a purified
enzyme preparation of
homoaconitase . -
pubmed
These three
HMG ' s from embryonic skeletal muscle and erythrocytes also comigrated on two - dimensional gels , employing an acid -
urea system in the first dimension and an
NaDodSO4 system in the second . -
pubmed
Interpretation of the two - dimensional gels suggested that the two low molecular weight proteins of this triplet arose from the
HMG 2 band of the acid -
urea gels . -
pubmed
On the other hand ,
HLA - Bw52 - Dw12 haplotype which was reported to be in strong linkage disequilibrium with an immune suppression gene for schistosomal adult worm
antigen was significantly decreased ( corrected P less than 0.005 ) . -
pubmed
Influence of endotoxin treatment on
dexamethasone induction of hepatic
phosphoenolpyruvate carboxykinase . -
pubmed
Furthermore , the induction of hepatic
PEPCK by
dexamethasone was inhibited by 1 h after endotoxin treatment , with enzyme activity falling to basal , noninduced levels by 6 h posttreatment . -
pubmed
Additional , but fewer
zinc - containing neurons were located in layers
II , III and deep layer VI of the ipsilateral neocortex . -
pubmed
In the cingulate cortex ,
zinc - containing neurons with striatal projections were found predominantly in the ipsilateral layers
II and III , with only few neurons in the ipsilateral layer VI and in the contralateral layers
II , III and VI . -
pubmed
Zinc has been found to modulate the response of many ligand- and voltage - gated ion channels , including both
GABA receptors and
NMDA- ,
AMPA- and
kainate - type glutamate receptors . -
pubmed
In this report , we demonstrate that a single
amino acid mutation of a
Tyr to a
Cys at position 723 ( Y723C ) in the
TM cytoplasmic domain of
CP - MAC is the principal determinant for the increased expression of
envelope glycoproteins on the cell surface . -
pubmed
Substituting other
amino acids , including
Ala ,
Ile , and
Ser , at this position produced increases in
surface envelope glycoproteins that were similar to that observed for the
Cys substitution , while a
Tyr - to -
Phe mutation produced a smaller increase . -
pubmed
To test this hypothesis , confluent endothelial cell monolayers were exposed for 24 h to 500 or 1,000 U of
TNF per milliliter of culture medium together with 20 microCi
Na2 35SO4 . -
pubmed
HPLC anion - exchange separation of proteoglycans secreted into media of control as well as
TNF - treated cultures revealed one major peak ( representing 95 % of total radioactivity ) and one minor peak ( representing 5 % of total radioactivity ) , which eluted at 0.6 and 0.9 M
NaCl , respectively . -
pubmed
TNF treatment caused a dose - dependent decrease in mRNA levels for the core proteins of
perlecan , a major
heparan sulfate proteoglycan (
HSPG ) , and
biglycan in endothelial cultures . -
pubmed
The
carboxyl terminus - truncated
cadherin (
nonfunctional cadherin ) has no cell adhesion activity probably because of its failure to associate with
cytoplasmic proteins called
alpha and beta catenin . -
pubmed
Taken together , we conclude that in the formation of the
cadherin - catenin complex , the mechanical association of
alpha catenin , especially its
carboxy - terminal half , with
E - cadherin is a key step for the
cadherin - mediated cell adhesion . -
pubmed
This article reports the complete structure of X , which was determined from the FAB -
MS and 2D NMR analysis of the
peracetylated LOS - III . -
pubmed
Sequential extraction was performed utilizing 1 ) 0.1 %
Triton X-100 , 2 ) 4 M
guanidine - HCl , and 3 )
papain digestion , to release species of hyaluronan progressively more tightly associated with tissue . -
pubmed
Hemimandibles were osteotomized perpendicular to the long axis between the third and fourth premolars , and segments were stabilized with 1 of 5 interdental fixation apparatuses : Erich arch bar ( EAB , n = 6 ) ; Stout loop ( SL , n = 6 ) ;
acrylic ( A , n = 6 ) ; Stout loop and acrylic (
SLA , n = 24 ) ; and Erich arch bar and acrylic ( EABA , n = 6 ) . -
pubmed
DNAase I and
micrococcal nuclease reveal drug - induced footprints with the central sequences ATAT , TATA and TTAA , but not AATT , suggesting that the ligand binds to the
dinucleotide TpA. -
pubmed
Intrinsic activity was demonstrated in vitro against a variety of
phosphotyrosine - containing substrates including
BIRK , the autophosphorylated cytoplasmic kinase domain of the
insulin receptor beta subunit . -
pubmed
The major criteria for purity of the
bmv fraction are : ( i ) It is enriched 15 - fold for a known plasma membrane marker ( immobilization
antigen ) while the marker activities for other membranes were all decreased . -
pubmed
The protein banding pattern of
bmv is generally similar to
cmv on
SDS - PAGE . -
pubmed
( ii ) It contains a
vanadate - sensitive Ca ( 2 +)- ATPase activity that has been suggested to be a plasma membrane
Ca2 + pump . -
pubmed
( iii ) The phospholipid ,
fatty acid , and
sterol composition of the bmv fraction are indicative of plasma membranes because they are qualitatively similar to
cmv . -
pubmed
This purified
bmv preparation is useful for studying the role of the body plasma membrane in
Ca2 + regulation , sensory transduction , protein and lipid trafficking , and plasma membrane fusion events . -
pubmed
The effect of L - RNA was dependent of the integrity of the polynucleotide chain and was not due to LPS contamination since its ability to induce MNCF and
IL-8 release was strongly reduced by
RNase but was not affected by
DNase or
polymyxin B. -
pubmed
The demonstration that
actinomycin - D and
cycloheximide inhibited the release of
MNCF and
IL-8 by L - RNA - stimulated macrophages confirms this assumption . -
pubmed
Fractionation of the total L - RNA by centrifugation on a 5 - 20 %
sucrose gradient showed that the L - RNA which sediments in the 4 - 5S region of the gradient is the only fraction capable of inducing the release of
MNCF from naive macrophages . -
pubmed
High - resolution crystal structure of
magnesium (
MgII)-
iron (
FeII ) hybrid
hemoglobin with liganded beta subunits . -
pubmed
The
deoxy hybrid has virtually the same structure as that of the native deoxy
HbA. -
pubmed
Both the
deoxy and
CO - liganded hybrids assumed a T quaternary structure characteristic of native
deoxy HbA. -
pubmed
No significant structural difference was found between the alpha subunits of the
CO - liganded hybrid and
deoxy HbA , while in the beta subunit , significant tertiary structural changes were confined to the
heme pocket . -
pubmed
On the other hand , when the two structures are compared with superposition of hemes , the nearest neighbors of
CO (
Fe ,
E11 Val and E7
His ) have shifted nearly to the same positions as those in
COHbA. -
pubmed
The persistence of this photoproduct and its high neuroactivity , resulting from blocking the
lambda - aminobutyric acid - gated chloride channel , suggest that it may be a significant contributor to the effectiveness of
fipronil . -
pubmed
Based upon the
CAPS , a standardized
post - traumatic stress disorder (
PTSD ) interview , findings from a cohort involving both subchronic and chronic
traumatic stress marginally favored
brofaromine over placebo ; however , not to a statistically significant degree . -
pubmed
Optical densities of
5HT IR and
VIP IR were increased by
MAOI treatment . -
pubmed
Phospholipase C - delta1 and
oxytocin receptor signalling : evidence of its role as an
effector . -
pubmed
Although the
oxytocin receptor modulates intracellular
Ca2 + ion levels in myometrium , the identities of signal
molecules have not been clearly clarified . -
pubmed
PLC - delta1 activity in the three - component preparation , as well as
PLC activity in solubilized membranes , was increased by
oxytocin in the presence of
Ca2 + and activated
Ghalpha (
GTP - bound Ghalpha ) . -
pubmed
Furthermore the stimulated
PLC - delta1 activity resulting from activation of
Ghalpha via the
oxytocin receptor was significantly attenuated by the selective
oxytocin antagonist
desGly - NH2d ( CH2 ) 5 [ Tyr ( Me ) 2,Thr4 ] ornithine vasotocin or
GDP . -
pubmed
These results indicate that
PLC - delta1 is the
effector for
Ghalpha - mediated
oxytocin receptor signalling . -
pubmed
Local delivery of
cefazolin is desired in conditions such as
osteomyelitis ,
soft - tissue infection and for prevention of
post - surgical
infections . -
pubmed
Prolonged reversible nerve blockade required in a number of clinical situations involving acute or chronic
pain such as
post - surgical
pain following herniorrhaphy and thoracotomy can be achieved with local delivery of
bupivacaine . -
pubmed
Therefore , P(FAD -
SA , 50:50 w / w ) may not be undergoing surface erosion , and the diffusion and dissolution properties of the drug in addition to erosion characteristics of the
polyanhydride appear to play a role in drug release . -
pubmed
Cefazolin implants released the drug for a sufficient duration for
osteomyelitis and
soft - tissue infection but the release was more prolonged than required for prevention of
post - surgical
wound infection . -
pubmed
, one encoding the
G6PD , a major enzyme regenerating anti - alkylating
GSH , and the other encoding
ALDH1 , which has a high activity for oxidation of
aldophosphamide derived from
4HC . -
pubmed
We believe that there are sufficient arguments to support controlled
iron supplementation in all athletes with low serum
ferritin levels . -
pubmed
Serum
leptin levels rose 2- to 10 - fold , with peak increases seen 8 - 16 h after injection of
CPA ( P & lt ; 0.05 ) . -
pubmed
In contrast ,
CPA did not alter steady state levels of adipose tissue
leptin mRNA . -
pubmed
Supporting the idea that stimulation of
leptin production is
A1AR mediated , pretreatment with the
A1AR antagonist
8 - cyclopentyl-1,3 - dipropylxanthine completely blocked increases in
leptin levels after
DPY treatment . -
pubmed
In cultures ,
CPA treatment increased
leptin secretion by 37 % ( P & lt ; 0.05 ) . -
pubmed
Carboxyatractylate (
CAtr ) , an inhibitor of
AAC , completely inhibits
oleate- or
laurate - stimulated
oxygen consumption linked to
succinate oxidation , while
GDP , an inhibitor of
PUMP , caused only a 30 % inhibition . -
pubmed
These results show that the
succinate - dependent
H ( 2 ) O ( 2 ) formation occurs at the level of
Complex I by a reverse electron transport . -
pubmed
The anionic form of
FFA appears to be shuttled by
AAC rather than
PUMP . -
pubmed
Galactosylation of
thiol group by
beta - galactosidase . -
pubmed
beta - Galactosidase catalyzed beta - galactosylation not only of a
hydroxyl group but also of a
thiol group in the condensation reaction of
D - galactose and
2 - mercaptoethanol . -
pubmed
Serum
aspartate aminotransferase activity , serum and milk
betahydroxybutyric acid concentrations , and milk fat - to - protein ratios were often elevated before the diagnosis of
DA , and these variables may be used to predict
DA . -
pubmed
Two novel macromolecular MRI contrast agents based upon generation-6
polyamidoamine dendrimers (
G6 ) of presumed similar molecular size , but of different molecular weight , were compared in terms of their blood retention , tissue distribution , and renal excretion . -
pubmed
The
G6A --( 1B4M - Gd )( 192 ) showed higher signal intensity in the kidney on the dynamic MR images and
brighter kidney images than
G6E --( 1B4M - Gd )( 256 ) . -
pubmed
In conclusion , the
G6A --( 1B4M - Gd )( 192 ) was observed to go through glomerular filtration more efficiently than
G6E --( 1B4M - Gd )( 256 ) resulting faster clearance from the blood and higher renal accumulation , even though both of
G6 - -1B4 M conjugates have almost similar molecular size and same chemical structure . -
pubmed
Role of
estrogen receptor beta in
estrogen action . -
pubmed
The importance of
androgen in females was never in doubt , it is after all the precursor of
estrogen as the substrate for
aromatase , the enzyme that produces
estrogen . -
pubmed
In addition , the tissue distribution of
androgen receptors suggests that
androgens themselves are important in the ovary , uterus , breast , and brain . -
pubmed
OBJECTIVES : Tapping mode atomic force microscopy (
AFM ) was used to investigate the surface changes of
collagen exposed to
phosphoric acid treatment . -
pubmed
Delayed
cerebral edema and fatal
coma after minor
head trauma : role of the
CACNA1A calcium channel subunit gene and relationship with
familial hemiplegic migraine . -
pubmed
Mutations in the
CACNA1A calcium channel subunit gene on chromosome 19 are associated with a wide spectrum of mutation - specific episodic and chronic
neurological disorders , including
FHM with or without
coma . -
pubmed
In all three subjects with delayed severe
edema , we found a C - to - T substitution resulting in the substitution of
serine for
lysine at codon 218 ( S218L ) in the
CACNA1A gene . -
pubmed
We conclude that the novel S218L mutation in the
CACNA1A calcium channel subunit gene is involved in
FHM and delayed fatal
cerebral edema and
coma after minor
head trauma . -
pubmed
Magnetic resonance imaging ( MRI ) demonstrated a reduction in the size of
T2 high lesions and
gadolinium enhancement in the thigh muscles in one case . -
pubmed
The in vitro responsiveness of PMNs stimulated with fMLP or
PAF was inversely correlated with indices of in vivo glycemic control ( fasting plasma
glucose and
glycated - hemoglobin levels ) . -
pubmed
The applicability of this hybrid approach was evaluated by calculating the absolute free energy of binding and free - energy changes for
amino acid substitutions in the complex between the
U1A spliceosomal protein and its cognate RNA hairpin . -
pubmed
Cyclooxygenase-2 (
COX-2 ) is an inducible enzyme homologous to
COX-1 that is responsible for production of
prostaglandins at sites of
inflammation . -
pubmed
PURPOSE : To determine if an association exists between postdilation restenosis and
heme oxygenase-1 (
HO-1 ) , which is induced by
balloon injury and inhibits neointimal formation through the action of endogenous
carbon monoxide . -
pubmed
A
dinucleotide repeat in the promoter region of the
HO-1 gene shows a length polymorphism that modulates the level of gene transcription . -
pubmed
Blood levels of
Ca , P ,
alkaline phosphatase ,
aluminium , iPTH and dialysis doses were measured every 4 months during the two years of follow - up . -
pubmed
Here we report that
APE1 has a
DNA exonuclease activity on mismatched
deoxyribonucleotides at the 3 ' termini of nicked or gapped DNA molecules . -
pubmed
The
exonuclease activity of
APE1 can remove the anti - HIV
nucleoside analogues
3 '- azido-3 '- deoxythymidine and
2 ' , 3 '- didehydro-2 ' , 3 '- dideoxythymidine from DNA , suggesting that
APE1 might have an impact on the therapeutic index of antiviral compounds in this category . -
pubmed
This oscillation parallels the 24 - min periodicity observed for the oxidation of
NADH by the external plasma membrane
NADH oxidase . -
pubmed
The
serotonin transporter (
5HTT ) is the molecule responsible for the high - affinity reuptake of
5HT from the synaptic cleft . -
pubmed
Among the 37 cases , 35 ( 94.6 % ) had total
bile acids elevation , 32 ( 86.5 % ) had serum
cholesterol elevation and 32 ( 86.5 % ) serum
IgM elevation , 34 ( 91.9 % ) were positive for
anti - mitochondrial antibody (
AMA ) and/or
AMA - M2 . -
pubmed
NONOates regulate
KCl cotransporter-1 and - 3 mRNA expression in vascular smooth muscle cells . -
pubmed
In this study , we used
NONOates as rapid and slow
NO releasers to provide direct evidence implicating
NO as a regulator of
KCC3 gene expression at the mRNA level . -
pubmed
In addition , we used the expression of
KCC3 mRNA to further investigate the mechanism of action of these
NO donors at the cellular level . -
pubmed
Treatment of VSMCs with rapid
NO releasers , like
NOC-5 and
NOC-9 , as well as with the direct
NO - independent soluble
guanylyl cyclase (
sGC ) stimulator
YC-1 , acutely increased
KCC3 mRNA expression in a concentration- and time - dependent manner . -
pubmed
The slow
NO releaser
NOC-18 had no effect on
KCC3 gene expression . -
pubmed
A specific
NO scavenger completely prevented the
NONOate - induced
KCC3 mRNA expression . -
pubmed
Inhibition of
sGC with
LY-83583 blocked the
NONOate- and
YC-1 - induced
KCC3 mRNA expression . -
pubmed
Lovastatin inhibits bone marrow - derived dendritic cell maturation and upregulates proinflammatory
cytokine production . -
pubmed
Lovastatin inhibited
MHC class II and
CD40 expression on DC in a dose - dependent manner , but had lesser effects on
CD16 ,
CD80 ,
CD86 , and
CD11b expression . -
pubmed
Nuclear extracts of
lovastatin treated DC had decreased
NF - kappaB DNA binding activity . -
pubmed
Lovastatin up - regulated DC pro - inflammatory
cytokine production induced by LPS as measured by intracellular cytokine staining , ELISA and cDNA microarrays . -
pubmed
These results indicate that
lovastatin may inhibit BM - DC maturation and up - regulate
cytokine production through a
mevalonate dependent pathway , and may cause adverse effects on either innate or adaptive immunity . -
pubmed
Also , the use of the radiopharmaceuticals , radioactive
thallium chloride-201 ,
methylene diphosphonate labelled with
technetium-99 m ,
somatostatin receptors labelled with
indium-111 , diagnostic procedures with the PET camera , the labelled antibodies to
CEA with
technetium-99 m and also the importance of the MRI and the US techniques are mentioned . -
pubmed
After
rhG - CSF , there were increases both in
TF antigen (
TF : Ag ) ( P=0.01 ) and
TF procoagulant activity ( TF : PCA ) ( P=0.06 ) plasma levels and in
TF :
Ag cytofluorimetric expression on
CD33 ( + ) cells ( P=0.04 ) . -
pubmed
Arachidonic acid ( AA ) metabolites derived from both the
lipoxygenase (
LOX ) and
cyclooxygenase (
COX ) pathways transduce a variety of signals related to cell growth . -
pubmed
The catalytic activity of
PARP was inhibited by the potent
isoindolinone - based
PARP inhibitor
INO-1001 or by
PARP-1 genetic deficiency . -
pubmed
The ability of the inhibitor to block the catalytic activity of
PARP was confirmed by immunohistochemical detection of
poly ( ADP - ribose ) , the product of the enzyme in the heart . -
pubmed
Nectin - like molecule-1 /
TSLL1 /
SynCAM3 : a neural tissue - specific immunoglobulin - like cell - cell adhesion
molecule localizing at non - junctional contact sites of presynaptic nerve terminals , axons and glia cell processes . -
pubmed
Nectins are
Ca2 + -independent
immunoglobulin - like cell - cell adhesion
molecules and comprise a family of four members . -
pubmed
Five
nectin - like
molecules (
Necls ) which have domain structures similar to those of
nectins have been identified and here we characterize
Necl-1 /
TSLL1 /
SynCAM3 , from now on referred to as
Necl-1 . -
pubmed
Necl-1 showed
Ca2 + -independent homophilic cell - cell adhesion activity . -
pubmed
It furthermore showed
Ca2 + -independent heterophilic cell - cell adhesion activity with
Necl-2 /
IGSF4 /
RA175 /
SgIGSF /
TSLC1 /
SynCAM1 from now on referred to as
Necl-2 ,
nectin-1 and
nectin-3 , but not with
Necl-5 or
nectin-2 . -
pubmed
These results indicate that
Necl-1 is a neural - tissue - specific
Ca2 + -independent
immunoglobulin - like cell - cell adhesion molecule which potentially has membrane - associated
guanylate kinase subfamily member - binding activity and localizes at the non - junctional cell - cell contact sites . -
pubmed
This defective
IL-10 production could be explained by a reduction in the
cAMP - dependent signalling pathway , which mediates
IL-10 production . -
pubmed
Fluorescence quenching experiments determined that purified
FhuD bound
hydroxamate - type siderophores . -
pubmed
The role of
Fhu in siderophore -
iron transport was also characterized . -
pubmed
A
fhu mutant ,
ACFhu1 , was equally sensitive to the
iron - dependent antibiotic
streptonigrin as the wild - type
strain , suggesting that
ACFhu1 was not reduced for intracellular
iron concentrations in the absence of exogenous siderophore . -
pubmed
However ,
ACFhu1 transported significantly less siderophore - bound
iron in
( 55 ) Fe accumulation assays . -
pubmed
The
small GTPase Rac cycles between the membrane and the cytosol as it is activated by
nucleotide exchange factors (
GEFs ) and inactivated by
GTPase - activating proteins (
GAPs ) . -
pubmed
Solubility in the cytosol is conferred by binding of
Rac to
guanine -
nucleotide dissociation inhibitors (
GDIs ) . -
pubmed
Thus , the major route for dissociation is conversion of membrane - bound
GTP -
Rac to
GDP -
Rac ; however , dissociation of
GTP -
Rac occurs at a detectable rate . -
pubmed
Overexpression of the
GEF Tiam1 unexpectedly decreased k(off ) for
wtRac , most likely by converting membrane - bound
GDP -
Rac back to
GTP - Rac . -
pubmed
We hereby present evidence that prolonged exposure to
CO ( 2 ) impairs developmental
retinal neovascularization through a mechanism involving increased
endothelial nitric oxide synthase and induction of a nitrative stress ; effects of
hypercapnia are independent of its
hyperaemic effects . -
pubmed
Moreover , we demonstrate that an in vivo nitrative stress associated with
retinal vasoobliteration results in nitration of
arachidonic acids into
trans - arachidonic acids ( TAAs ) , which can act as mediators of nitrative stress by causing
microvascular degeneration by inducing expression of the antiangiogenic factor
thrombospondin-1 . -
pubmed
Characterization and tissue - specific expression of two lepidopteran
farnesyl diphosphate synthase homologs : implications for the biosynthesis of
ethyl - substituted juvenile hormones . -
pubmed
The biogenesis of these JHs requires the synthesis of
ethyl - substituted farnesyl diphosphate (
FPP ) by
FPP synthase (
FPPS ) . -
pubmed
Type - I FPPSs display unique active site substitutions , including several in and near the first
aspartate - rich motif , whereas type - II proteins have a more " conventional " catalytic cavity . -
pubmed
Increased generation of
oxygen - derived free radicals resulted in the
Ras mutant cells accumulation of lipofuscin - like pigments during ageing . -
pubmed
In contrast , the
Ras mutant cells contained decreased levels of
alpha tocopherol even in the young cells . -
pubmed
Protein
nitrotyrosine , a marker of the reactive
nitrogen species , has significantly increased in the senescent
Ras mutant cells . -
pubmed
Overexpression of
antiapoptosis protein Bcl-2 in
AML cells was directly correlated with
p53 expression and phosphorylation on
serine residues 15 , 46 , and 392 . -
pubmed
Use of integrated
FDG PET /
CT imaging in
pulmonary carcinoid tumours . -
pubmed
OBJECTIVE : To evaluate the usefulness of integrated
FDG PET /
CT in
pulmonary carcinoid tumours . -
pubmed
CONCLUSIONS :
( 18 ) F - fluorodeoxyglucose PET /
CT imaging improves accurate localization of metabolic activity and thus the interpretation of
pulmonary lesions on
CT . -
pubmed
In 37 % of patients ,
nonnucleoside reverse transcriptase inhibitor drug concentrations were still detectable 1 week after stopping . -
pubmed
The
type IIa Na (+)/ P ( i ) cotransporter (
NaPi - IIa ) plays a key role in the reabsorption of inorganic
phosphate (
P ( i ) ) in the renal proximal tubule . -
pubmed
Laboratory parameters measured were blood
glucose , urinary
ketones ,
electrolytes ,
urea creatinine , arterial blood gas (
ABG ) and infectious screen . -
pubmed
The median time to normalize
ABG was 19 hr ( 5.3 - 39 ) while the median time for the urinary
ketones to disappear was 1day ( 1 - 3 ) . -
pubmed
Pael receptor is involved in
dopamine metabolism in the nigrostriatal system . -
pubmed
We report here the X - ray structures of the periplasmic sensor domain of
CitA in the
citrate - free and
citrate - bound states . -
pubmed
A screen of candidate cell adhesion
molecules revealed that the expression of
connexin ( Cx )- 43 correlated with the age - dependent effect of cell contact in cell pair experiments . -
pubmed
IMCL levels and skeletal muscle oxidative capacity were determined in vivo , using localized
( 1 ) H magnetic resonance spectroscopy (
MRS ) and dynamic
( 31 ) P MRS , respectively . -
pubmed
strain
GIN1 ) was previously isolated on the basis of its strong adherence to
coal fly ash ( CFA ) and
titanium dioxide particles from CFA sedimentation ponds of an electrical power plant in Israel . -
pubmed
The interaction of the bacterium with
oxides has been shown to be mediated by a cell surface protein designated
TiBP (
titanium binding protein ) involving primarily strong , non - electrostatic forces . -
pubmed
Vision - related adverse effects of commonly used medications , such as
amiodarone or
phosphodiesterase inhibitors , should be considered when evaluating vision problems . -
pubmed
Root - end cavities in groups A and B were filled with
MTA and NEC , respectively , and were then stored in 100 % humidity for 24 h. The samples of groups 1 and 2 were , respectively , immersed in normal saline (
NS ) and phosphate buffer saline solutions for 1 week . -
pubmed
Contraction of both veins and arteries were significantly inhibited by the
5 - HT ( 2A ) receptor antagonist
ketanserin . -
pubmed
No effect on contraction was seen with the
cyclooxygenase inhibitor
indomethacin , the
alpha ( 1 ) receptor antagonist
prazosin or the
angiotensin AT ( 1 ) receptor antagonist
saralasin neither in veins nor arteries . -
pubmed
We have identified the periplasmic lipoprotein
HbpA , a protein hitherto implicated in
heme acquisition , as the cognate
PBP that specifically binds reduced (
GSH ) and
oxidized glutathione (
GSSG ) forms of
glutathione with physiologically relevant affinity , while it exhibits marginal binding to
hemin . -
pubmed
Dissection of the ligand preferences of
HbpA showed that
HbpA does not recognize bulky
glutathione S conjugates or
glutathione derivatives with C - terminal modifications , consistent with the need for selective import of useful forms of
glutathione and the concomitant exclusion of potentially toxic
glutathione adducts . -
pubmed
In addition to several clinical variables , several biomarkers were significant predictors of
death , including log - transformed concentrations of
hemoglobin ( hazard ratio=0.77 ; P & lt ; 0.001 ) , soluble
ST2 ( hazard ratio=1.38 ; P & lt ; 0.001 ) , and
amino - terminal pro - B - type natriuretic peptide ( hazard ratio=1.19 ; P & lt ; 0.001 ) . -
pubmed
Reaction with the substrate quantitatively affords a monolayer of electronically intact clusters doubly linked to the surface via
Au - S bonds , as demonstrated by a combination of
STM , XAS / XMCD and XPS studies . -
pubmed
Our sequence analysis showed that
B12 - dependent and B12 - independent
glycerol dehydratases are not related , suggesting that they evolved from different ancestors . -
pubmed
Arachidonoyl ethanolamide (
AEA)-induced apoptosis is mediated by J - series
prostaglandins and is enhanced by
fatty acid amide hydrolase (
FAAH ) blockade . -
pubmed
A previous report from our laboratory showed that
AEA induced cell
death in a
cyclooxygenase-2 (
COX-2)-dependent manner and in this report our data indicate that
AEA - induced apoptosis is mediated by
COX-2 metabolic products of the J - series . -
pubmed
Using antagonists of
cannabinoid receptor 1 ,
cannabinoid receptor 2 , or
transient receptor potential cation channel , subfamily V , member 1 , it was observed that cannabinoid receptor inhibition did not block
AEA - mediated cell
death . -
pubmed
In contrast , an inhibitor of
fatty acid amide hydrolase (
FAAH ) potentiated
AEA - induced J - series PG synthesis and apoptosis . -
pubmed
These results suggest that the metabolism of
AEA to
J - series PGs regulates the induction of apoptosis in cells with elevated
COX-2 levels . -
pubmed
Our data further indicate that the proapoptotic activity of
AEA can be enhanced by combining it with an inhibitor of
FAAH . -
pubmed
As such ,
AEA may be an effective agent to eliminate
tumor cells that over - express
COX-2 . -
pubmed
The structures of these compounds were confirmed by a chemical analysis and spectrophotometric methods ( UV ,
1H- ,
13C - NMR , ESI -
MS ) . -
pubmed
No
amino acid substitutions predicted to be functionally damaging were found in
TLR7 . -
pubmed
In the case of
dioxin - like compounds ( DLCs ) , it has been hypothesized that single
nucleotide polymorphisms ( SNPs ) in genes associated with
aryl hydrocarbon receptor (
AHR)-regulated pathways may result in greater susceptibility to
DLC toxicity . -
pubmed
Confocal
calcium imaging was used to detect spontaneous
calcium release (
calcium sparks and waves ) from the
SR . -
pubmed
Inhibition of
SR calcium uptake reduced the
calcium transient ( F / F(0 ) ) from 1.77 + -0.17 to 1.12 + -0.18 ( p = 0.002 ) and abolished
calcium sparks and waves . -
pubmed
An N - linked glycosylation system encoded by the
pgl gene cluster mediates the synthesis of a rigidly conserved
heptasaccharide that is attached to protein substrates or released as free
oligosaccharide in the periplasm . -
pubmed
coli
pEtN transferases can mediate the addition of
pEtN to
N - glycans . -
pubmed
The emission shows a decrease in intensity with an increase in external temperature , however contrary to the normal behavior of
Ho ( 3 + ) , no significant change in the
FIR ( fluorescence intensity ratio ) of ( 5)F(4 ) ( 5)I(8 ) and
( 5 ) S ( 2 ) ( 5 ) I ( 8 ) transitions is noted in the present host . -
pubmed
CONCLUSIONS : These data suggest an important role for
CD44 in the context of
tamoxifen - resistance where it may augment cellular response to
erbB ligands and HA , factors that are reported to be present within the
tumour microenvironment in vivo . -
pubmed
Epac2 mediates cardiac beta1 - adrenergic - dependent sarcoplasmic reticulum
Ca2 + leak and
arrhythmia . -
pubmed
BACKGROUND :
beta - Adrenergic receptor (
beta - AR ) activation can provoke
cardiac arrhythmias mediated by
cAMP - dependent alterations of
Ca ( 2 + ) signaling . -
pubmed
However ,
cAMP can activate both
protein kinase A and an exchange protein directly activated by
cAMP (
Epac ) , but their functional interaction is unclear . -
pubmed
In heart , selective
Epac activation can induce potentially arrhythmogenic sarcoplasmic reticulum (
SR )
Ca ( 2 + ) release that involves
Ca ( 2 +)/ calmodulin - dependent protein kinase II (
CaMKII ) effects on the
ryanodine receptor (
RyR ) . -
pubmed
METHODS AND RESULTS : We tested whether physiological
beta - AR activation causes
Epac - mediated
SR Ca ( 2 + ) leak and
arrhythmias and whether it requires
Epac1 versus
Epac2 ,
beta ( 1 )- AR versus
beta ( 2 )- AR , and
CaMKIIdelta - dependent phosphorylation of
RyR2 - S2814 . -
pubmed
beta - AR activation with
protein kinase A inhibition mimicked
8 - CPT effects on
SR Ca ( 2 + ) leak and was prevented by blockade of
beta ( 1 )- AR but not
beta ( 2 )- AR . -
pubmed
CaMKII inhibition (
KN93 ) and genetic ablation of either
CaMKIIdelta or
CaMKII phosphorylation on
RyR2 - S2814 prevented
8 - CPT - induced
SR Ca ( 2 + ) leak . -
pubmed
CONCLUSIONS :
beta ( 1 )- AR activates
Epac2 to induce
SR Ca ( 2 + ) leak via
CaMKIIdelta - dependent phosphorylation of
RyR2 - S2814 . -
pubmed
Impaired glycolytic metabolism causes
chondrocyte hypertrophy - like changes via promotion of
phospho -
Smad1 / 5 / 8 translocation into nucleus . -
pubmed
Interestingly , combinational treatment with
NaF and
lactate enhanced translocation of
phospho -
Smad1 / 5 / 8 to the nucleus , as well as gene expression of
ALP ,
VEGF ,
COL10a1 , and
matrix metalloproteinase13 (
MMP13 ) , which were the markers of late mature and hypertrophic chondrocytes . -
pubmed
CONCLUSIONS : These findings suggest that decreased
ATP production by
NaF promotes
hypertrophy - like changes via activation of
phospho -
Smad1 / 5 / 8 in the presence of
lactate . -
pubmed
Azithromycin reduces and prevents broncholaveolar lavage (
BAL )
neutrophilia after lung transplantation ( LTx ) . -
pubmed
Azithromycin reduced both %
BAL neutrophilia and
IL-17 + cells ( both p = 0.016 ) in the LB group . -
pubmed
Moreover ,
azithromycin significantly decreased the number of
IL-17 + cells in the airway wall , which may further explain its effect on
BAL neutrophilia . -
pubmed
Over - activation of microglia cells in the brain contributes to neurodegenerative processes promoted by the production of various
neurotoxic factors including pro - inflammatory
cytokines and
nitric oxide . -
pubmed
Furthermore , mitochondrial localization of
dynamin - related protein 1 (
Drp1 ) ( a key regulator of mitochondrial fission ) was increased and accompanied by de - phosphorylation of
Ser637 in
Drp1 . -
pubmed
Interestingly , inhibition of LPS - induced mitochondrial fission and reactive
oxygen species ( ROS ) generation by Mdivi-1 and
Drp1 knock - down attenuated the production of pro - inflammatory mediators via reduced
nuclear factor kappa - light - chain - enhancer of activated B cells (
NF - kappaB ) and
mitogen - activated protein kinase (
MAPK ) signaling . -
pubmed
Likewise ,
ERK phosphorylation was markedly enhanced in PAF - stimulated VSMCs , and this was attenuated by
WEB2086 , but not by
EGF receptor inhibitor , demonstrating the specificity of
PAF receptor (
PAFR ) in
PAF - induced
ERK phosphorylation . -
pubmed
In addition ,
PAF - induced
ERK phosphorylation and
MMP-2 production were significantly attenuated by
beta - arrestin2 depletion . -
pubmed
Taken together , the study shows that
PAF enhances
MMP-2 production in VSMCs via a
beta - arrestin2 - dependent
ERK signaling pathway . -
pubmed
We assessed the temporal profile of
alanine aminotransferase (
ALT ) ,
bilirubin (
Bil ) , and
alkaline phosphatase (
ALP ) . -
pubmed
Sox2 promotes
tamoxifen resistance in
breast cancer cells . -
pubmed
Here , we examined their role in the development of resistance to the
oestrogen receptor antagonist
tamoxifen . -
pubmed
Tamoxifen - resistant cells were enriched for stem / progenitors and expressed high levels of the stem cell marker
Sox2 . -
pubmed
Silencing of the
SOX2 gene reduced the size of the stem / progenitor cell population and restored sensitivity to
tamoxifen . -
pubmed
Conversely , ectopic expression of
Sox2 reduced
tamoxifen sensitivity in vitro and in vivo . -
pubmed
Gene expression profiling revealed activation of the Wnt signalling pathway in
Sox2 - expressing cells , and inhibition of Wnt signalling sensitized resistant cells to
tamoxifen . -
pubmed
Together , these results suggest that development of
tamoxifen resistance is driven by
Sox2 - dependent activation of Wnt signalling in
cancer stem / progenitor cells . -
pubmed
However , some
cancer cells lack
asparagine synthetase (
ASNS ) , the key enzyme for
asparagine synthesis . -
pubmed
Asparaginase (
ASNase ) contributes to eradication of
acute leukemia by decreasing
asparagine levels in serum and cerebrospinal fluid . -
pubmed
ASNase cotreatment significantly enhanced
gemcitabine or
etoposide cytotoxicity against
glioblastoma cells . -
pubmed
However , combinatorial therapy with
ASNase and
temozolomide resulted in significant growth suppression for an extended duration of time . -
pubmed
Bis - styrylbenzenes have been previously reported as potential
Abeta imaging agents . -
pubmed
To further explore their potency as
( 19 ) F MRI contrast agents we synthetized several novel
fluorinated bis - styrylbenzenes and studied their fluorescent properties and
amyloid - beta binding characteristics . -
pubmed
Unfortunately initial
post - mortem
( 19 ) F NMR studies showed that these compounds as yet do not warrant further MRI studies due to the reduction of the
( 19 ) F signal in the environment of the brain . -
pubmed
Twenty pairs of
Zn - contaminated soils ( contaminated and reference soils ) were collected on the field , and their enzymatic
FS (
arylsulfatase ,
protease ,
phosphatase and
urease ) and metal fractions ( total and bioavailable ) were analyzed . -
pubmed
New RSSI - based and existing
FS scores were calculated for each
enzyme and correlated to the
Zn fractions . -
pubmed
One of the new RSSI - based scores was well correlated with the bioavailable labile
Zn concentration for the
arylsulfatase ,
phosphatase and
urease ( coefficients of regression higher than 0.50 ) . -
pubmed
Other
FS scores were correlated to labile
Zn for only one
enzyme , which varied according to the score . -
pubmed
The new RSSI - based score thus better attributed
Zn toxicity to field - collected soils than other
FS scores . -
pubmed
Alanine substitution of the conserved residue H98 in
prM disrupts the switch by inhibiting dissociation of M from E at low pH , resulting in impaired
prM processing and decreased virus infectivity . -
pubmed
Selenadiazole derivatives as potent
thioredoxin reductase inhibitors that enhance the radiosensitivity of
cancer cells . -
pubmed
The redox enzyme
thioredoxin reductase (
TrxR ) plays a vital role in restoring cellular
thiol redox balance disrupted by radiation - induced reactive
oxygens species ( ROS ) generation and oxidative damage . -
pubmed
In this study , a series of
1,2,5 - selenadiazoles have been synthesized and identified as highly effective inhibitors of
TrxR to disrupt the intracellular redox balance , and thus significantly enhanced the sensitivity of
cancer cells to X - ray . -
pubmed
Interestingly , we also found the interaction sites between
1,2,5 - selenadiazole and the model peptide of
TrxR , which can be confirmed by MALDI - ToF -
MS . -
pubmed
These results clearly demonstrate
TrxR as a potential target for therapy of radioresistant
cancers , and
selenadiazole derivatives may be attractive radiosensitizing agent by targeting
TrxR. -
pubmed
AIM :
Oral contraceptive use influences the risk for certain
cancers . -
pubmed
We discuss the current understanding of
IPF and
BPF disease development , from the contribution of alveolar epithelial cells and
inflammation to the role of fibroblasts and
cytokines , and draw conclusions about what we have learned from the intratracheal
bleomycin model of
lung fibrosis . -
pubmed
It was seen that
CDHA coatings remarkably reduced the mass loss of
AZ31 alloy after 7days of immersion in
SBF . -
pubmed
Mutagenesis of the
cysteine and
glutamate residues in the N - terminal metal - binding domain ( N - MBD ) impaired the function of
TaHMA2 . -
pubmed
These data suggest that
cysteine and
glutamate residues are important for the metal - binding / translocation function of
TaHMA2 . -
pubmed
The GO was coated with hydrophilic branched
polyethyleneimine (
BPEI ) , while Concanavalin A ( Con A ) as a model
lectin protein was employed to fabricate the functionalized composites to evaluate the feasibility of this strategy . -
pubmed
Cell Polarity Kinase
MST4 Cooperates with
cAMP - dependent Kinase to Orchestrate
Histamine - stimulated Acid Secretion in Gastric Parietal Cells . -
pubmed
A coupling protein is
ezrin , whose phosphorylation at
Ser-66 by
PKA is required for parietal cell activation . -
pubmed
Here we show that
PKA cooperates with
MST4 to orchestrate
histamine - elicited acid secretion by phosphorylating
ezrin at
Ser-66 and
Thr-567 . -
pubmed
Histamine stimulation activates
PKA , which phosphorylates
MST4 at
Thr-178 and then promotes
MST4 kinase activity . -
pubmed
Importantly ,
MST4 is important for acid secretion in parietal cells because either suppression of
MST4 or overexpression of non - phosphorylatable
MST4 prevents the apical membrane reorganization and
proton pump translocation elicited by
histamine stimulation . -
pubmed
Ribavirin alone , or in combination with
interferon , has been the mainstream therapy for
HCV infection after transplantation . -
pubmed
Finally , the effect of
piperine on
Na (+ ) and Ca ( 2 + ) channels were evaluated using the whole cell patch
clamp techniqueOur results revealed that
piperine decreased mortality in the
MES - induced
seizure model . -
pubmed
Finally , whole cell patch
clamp analysis suggested an inhibitory effect of
piperine on
Na (+ ) channels . -
pubmed
Together , our data suggest
Na (+ ) channel antagonist activity as a contributor to the complex anticonvulsant mechanisms of
piperine . -
pubmed
We identified a series of conserved
amino acid residues and motifs that are likely to play a major role for the function of
MLO proteins . -
pubmed
Possibly , four conserved water molecules stabilize
oxyanion hole in unliganded state , but they vacate these positions when the
enzyme (
hGMPS)-substrate complex is formed . -
pubmed
The phase of oscillation in per expression was found to commence 3.5h earlier under
WC 25.5:24.5 C as compared to WC 29:21 C. This difference was in close agreement with the eclosion time difference between the two temperature cycles , suggesting that the mechanism that responds to the temperature amplitude involves the circadian
clock . -
pubmed
The present work considers the possibility of doing the same by using the basic
C2 units of
carbo - meric architectures . -
pubmed
The
C2 units are shown to stabilize the singlet biradical by spin delocalization , two of them playing approximately the same role as one radical - insulating
1,4 phenylene moiety . -
pubmed
Identification of Toxoplasma
TgPH1 , a
pleckstrin homology domain - containing protein that binds to the
phosphoinositide PI ( 3,5 ) P2 . -
pubmed
Here , by using a
phosphoinositide pull - down assay , we identified
TgPH1 in Toxoplasma a protein conserved in many apicomplexan parasites . -
pubmed
TgPH1 binds specifically to
PI ( 3,5 ) P2 , shows punctate intracellular localization , but plays no vital role for tachyzoite growth in vitro . -
pubmed
Therefore , our study reveals an unusual feature of
TgPH1 which binds preferentially to
PI ( 3,5 ) P2 . -
pubmed
Rapa was released from the
PLGA nanospheres incorporating
rapamycin (
Rapa - PLGA - NS ) with time while the Rapa -
PLGA -
NS were hydrolytically degraded . -
pubmed
The
microtubule - associated protein 1 light chain (
LC3 ) of an autophagy marker was significantly high for the
Rapa -
PLGA -
NS group compared with the free
Rapa group even after four days incubation . -
pubmed
RESULTS : Diet - tissue isotope enrichment ( trophic enrichment factor ,
TEF ) values between dietary herring and seal tissues for
carbon ( Delta13 C ) were +0.7 % for red blood cells , +1.9 % for hair and +1.1 % for muscle . -
pubmed
An HRAM LC -
MS method was validated for quantitative analysis of 26
steroid metabolites in hydrolyzed urine samples . -
pubmed
CONCLUSIONS : Our HRAM LC -
MS assay successfully quantifies 26
steroids in urine . -
pubmed
Further investigation revealed that
HDAC1 , a
histone deacetylase that was abnormally overexpressed in
hepatocellular carcinoma , could bind to the miR-195 promoter via
Smad3 and cause hypoacetylation in the
histones associated with the miR-195 promoter in
hepatoma cells . -
pubmed
BACKGROUND : Nutritional choices , which include the source of dietary
fatty acids (
FA ) , have an important significant impact on
coronary artery disease (
CAD ) . -
pubmed
On the other hand ,
catalase activity ,
conjugated dienes and
malondialdehyde were increased . -
pubmed
Furthermore , divergent associations of these
Trans FA accumulations were observed with
low - density lipoprotein -
cholesterol/
high - density lipoprotein -
cholesterol (
LDL - C /
HDL - C ) ratio ,
Apolipoprotein B (
ApoB ) , lipid peroxidation parameters , high - sensitivity
C Reactive Protein (
hs - CRP ) ,
Interleukin 6 (
IL-6 ) ,
tumor necrosis factor alpha (
TNF - alpha ) and Gensini score . -
pubmed
We aimed to evaluate whether pretransplant
aortic arch calcification ( AoAC ) can predict
post - transplant cardiovascular or cerebrovascular events ( CVEs ) , and to assess its association with
post - transplant plasma
calcium levels and renal function in one - year follow - up . -
pubmed
Zhang and colleagues ' study sheds light on the development of
magnesium pills or
calcitonin gene - related peptide - delivery system for the prevention or treatment of
osteoporosis . -
pubmed
Ag accumulation and
bacterial infection in PAT demonstrate that
PAT not only provides energy and regulatory factors , but can also directly participate in immune protection , indicating a new immune function of
PAT for host immunity . -
pubmed
Ombitasvir /
paritaprevir /
ritonavir +
dasabuvir +
ribavirin in
HCV genotype 1 infected patients who failed previous
protease inhibitor therapy . -
pubmed
Accordingly ,
PARP3 interacts with and
ADP - ribosylates
GSK3beta , a positive regulator of
Rictor ubiquitination and degradation . -
pubmed
Here we show that
Takinib , a highly discriminatory inhibitor of
transforming growth factor Beta - activated kinase 1 (
TAK1 ) , selectively and potently reduces
TNF production in pro - inflammatory THP-1 macrophages . -
pubmed
A complete survey of 110
cytokines , showed robust loss of proinflammatory
cytokine responsiveness to lipopolysaccharide ( LPS ) and
interferon gamma (
IFNgamma ) challenge in response to
Takinib . -
pubmed
The mechanisms of action of
Takinib was recapitulated in
TAK1 KO macrophages . -
pubmed
Furthermore ,
Takinib blocked the ability of exogenously added LPS to promote phosphorylation of ,
c - Jun ,
p38 protein kinases as well as downstream
transcription factors regulated by
nuclear factor kappa - light - chain - enhancer of activated B cells (
NFkappaB ) . -
pubmed
Our findings demonstrate that
Takinib has utility in the treatment inflammatory disease by locally suppressing
TNF production from invading macrophages . -
pubmed
The group treated with
meloxicam presented the highest rise in the
GPx level ( p = 0.052 ) . -
pubmed
Histone demethylase KDM6B regulates
1,25 - dihydroxyvitamin D3 - induced senescence in
glioma cells . -
pubmed
In this study , we show that
1,25 - dihydroxyvitamin D3 ( the active form of
vitamin D3 ) induces senescence of
glioma cells and increases the expression of senescence markers ,
INK4A and
cyclin - dependent kinase inhibitor 1A (
CDKN1A ) . -
pubmed
1,25 - Dihydroxyvitamin D3 also upregulates the expression of
histone demethylase ,
KDM6B. -
pubmed
Knockdown of
KDM6B attenuates
1,25 - dihydroxyvitamin D3 - induced senescence and upregulation of
INK4A and
CDKN1A. -
pubmed
PURPOSE OF REVIEW : This review outlines recent discoveries on the crosstalk between
oxygen metabolism and
iron homeostasis , focusing on the role of
HIF-2 (
hypoxia inducible factor-2 ) in the regulation of
iron metabolism under physiopathological conditions . -
pubmed
RECENT FINDINGS : The importance of the
hepcidin /
ferroportin axis in the modulation of intestinal
HIF-2 to regulate
iron absorption has been recently highlighted . -
pubmed
Latest advances also reveal a direct titration of the
bone morphogenetic proteins by the
erythroferrone contributing to liver
hepcidin suppression to increase
iron availability . -
pubmed
Materials and methods : The
sinus defects were grafted with
rhBMP-2+ACS ( Group A ) ,
rhBMP-2 - coated TCP ( Group B ) , and platelet - rich
fibrin - mixed TCP ( Group C ) . -
pubmed
On the converse , stimuli that suppress growth such as
testosterone depletion or acute aerobic exercise increased
Arrdc2 and
Arrdc3 expression in skeletal muscle . -
pubmed
These data supplement important technical and biological information on immune function of
Rap2a in macrophages activated by
LPS , recently reported by us ( Carvalho et al . -
pubmed
In sum , we report alteration of
UNC45A localization in the setting of chemotherapeutic treatment of cells with
paclitaxel , and localization of
UNC45A to MTs both in vitro and in vivo . -
pubmed
The opioid epidemic currently plaguing the United States has been exacerbated by an alarming rise in fatal
overdoses as a result of the proliferated abuse of synthetic
mu opioid receptor (
MOR ) agonists , such as
fentanyl and its related analogues . -
pubmed
The study discusses thermodynamic parameters , including changes in Gibbs free energy , entropy , and enthalpy , for the adsorption of
Ni2 + and
Pb2 + on
DSP , and revealed that the adsorption process was spontaneous and exothermic under natural conditions . -
pubmed
The maximum
Ni2 + and
Pb2 + adsorption capacities of
DSP were 33.02 and 36.50 mg / g , respectively . -
pubmed
The primary result was the percentage of bone - to - implant contact (
BIC ) around the
titanium implants with or without a
strontium - coated surface . -
pubmed
CONCLUSION : Nine of 11 studies reported that
titanium implants coated with
strontium showed significantly higher
BIC ( P & lt ; .001 to P & lt ; .05 ) . -
pubmed
A bio - inspired ,
ATP - driven nucleation growth assembly is demonstrated using an amphiphilic
naphthalene diimide (
NDI ) derivative appended with
guanidinium receptors to promote specific salt - bridge type interaction with
nucleotide phosphates . -
pubmed
Furthermore , overexpression of a
phospho - mimetic S175E
AMOT mutant , which has been established to enhance
AMOT -
YAP binding , increases
beta - catenin activity and rescues neurogenesis on stiff substrates . -
pubmed
SP - D showed a negative correlation with percutaneous
oxygen saturation and positive correlations with serum
lactate dehydrogenase , Krebs von den Lungen ( KL)-6 , and percentage of lymphocytes in bronchoalveolar lavage (
BAL ) . -
pubmed
According to the World Health Organization , there will be a return to the pre -
penicillin era by 2050 if no new antimicrobials are discovered . -
pubmed
As such ,
bilirubin may be marker for elevated risk of poor
post - operative outcomes and should be more frequently measured after cardiac surgery . -
pubmed
Some clinical investigations demonstrated that
rifampin pretreatment decreased exposure of
OATP1B substrates , while other studies found no differences , and the potential involvement of other mechanisms in these observed DDIs can not be definitively ruled out . -
pubmed
Gt1 could only function to maintain
zinc homeostasis in
glycerol , while the block of
Gt1 function might lead to
Zrt1 upregulation in
glucose . -
pubmed
CONCLUSIONS : The
zinc transport capabilities of
Gt1 and
Zrt1 were identified in vivo and in vitro . -
pubmed
As a crucial regulator of
ABA signaling ,
ABSCISIC ACID - INSENSITIVE5 (
ABI5 ) is involved in many aspects of plant growth and development . -
pubmed
Nevertheless , whether
ABI5 regulates
anthocyanin biosynthesis has not been reported . -
pubmed
A series of biochemical tests showed that
MdABI5 specifically interacts with
MdbHLH3 , a positive regulator of
anthocyanin biosynthesis . -
pubmed
Moreover ,
MdABI5 acted as an enhancer in the
ABA - promoted interaction between
MdMYB1 and
MdbHLH3 . -
pubmed
Finally , antisense suppression of
MdbHLH3 significantly reduced
anthocyanin biosynthesis promoted by
MdABI5 , indicating that
MdABI5 - promoted
anthocyanin biosynthesis was dependent on
MdbHLH3 . -
pubmed
Taken together , our data suggest that
MdABI5 plays a positive role in
ABA - induced
anthocyanin biosynthesis by modulating the
MdbHLH3 -
MdMYB1 complex . -
pubmed
Our work enriches the functional studies of
ABI5 and further broadens the regulatory network of
ABA - mediated
anthocyanin biosynthesis , providing new insights for further study of the transcriptional regulatory mechanisms of
anthocyanin biosynthesis . -
pubmed
We assessed the number of filled prescriptions of inhaled
corticosteroids ( ICS ) , ICS / long - acting
beta2 agonists ( LABA ) , and short - acting
beta2 agonists ( SABA ) , as well as the number of days ' supply of oral
corticosteroid (
OCS ) from the 20th week of pregnancy until delivery . -
pubmed
Astrocytes release molecules such as
thrombospondin ,
hevin , secreted protein acidic rich in
cysteine , etc . -
pubmed
After bilateral ureteral ligation , serum
granulocyte - macrophage colony - stimulating factor levels were increased and in vitro
tritiated thymidine suicide studies showed an increased proliferative rate of the CFU - c. Since
azathioprine is a predominantly cell cycle - specific agent , we suggest that increased sensitivity to
azathioprine is related to the increased proliferative rate of the CFU - c. The findings provide a rationale for a clinical policy of
azathioprine reduction when there is
depressed renal function . -
pubmed
At 10(-7 ) M ,
Iso produced approximately maximal responses at all ages in the ECH but elicited only minimal responses at all ages in the
ERH , approximately ten times this concentration being required to produce maximal responses in the
ERH . -
pubmed
Thyroidectomy and
thyroxine - injection markedly altered
fibrinogen metabolism : thyroid hormone accelerated
fibrinogen catabolism but also stimulated synthesis . -
pubmed
Further observations on the effects of
S - triazine derivatives on
purine metabolizing enzymes . -
pubmed
Elution volume from a Sephadex LH-20 column , development in three ascending paper chromatographic (
APC ) systems , acetylation and development in a fourth
APC system followed by gas liquid chromatography ( GLC ) of the acetylated products tentatively identified the radioactive - metabolites as
5alpha - androstan-17beta - ol-3 - one (
DHT ) ,
5alpha - androstan-3alpha,17beta - diol (
3alphaol ) and
5alpha - androstan-3beta,17beta - diol (
3beta - ol ) . -
pubmed
At 40 degrees , pH 8.0 , and in the presence of 10 mM
Ca2 + , V was found to be about 4 X 10(3 ) mumol min(-1 ) mg of
protein(-1 ) . -
pubmed
Each component was inactive by itself , but
cobaltochelatase activity was reconstituted upon mixing
CobN and
CobST . -
pubmed
Spectroscopic data revealed that the reaction product was
cob ( II ) yrinic acid a ,
c - diamide , and experiments with a coupled - enzyme incubation system containing both
cobaltochelatase and
cob ( II ) yrinic acid a , c - diamide reductase ( F. Blanche , L. Maton , L. Debussche , and D. Thibaut , J. Bacteriol . -
pubmed
She was initially treated with
cytokines plus
5 - fluorouracil derivative , resulting in a complete remission lasting over a year . -
pubmed
Values for the fractional excretion of
glucose were low and consistent , with or without
angiotensin II . -
pubmed
Although the unbound fraction ( fu ) of
chlorothiazide was unchanged between treatments , the renal ( CLr ) and the secretion clearances were reduced by about 50 % in the presence of
angiotensin II ; the excretion ratio [
ER = CLr/(fu . -
pubmed
Transport parameters ( + /- SD ) were obtained and the corrected intrinsic secretory clearance [ ( Vmax / GFR)/Km ] of
chlorothiazide was 123 + /- 18 without
angiotensin II vs. 72.8 + /- 30.0 with
angiotensin II . -
pubmed
From the study of absorption and fluorescence spectra of the complex "
Hoechst-33258"--DNA at different pH it is shown that
AT -- specific complex with DNA is formed by the neutral dye molecule , whereas the cationic state of the dye molecule forms the nonspecific complex . -
pubmed
A secondary - structure comparison between the
nucleotide - binding domain of
NAD(+)-dependent enzymes and that of
NADP (+)- dependent enzymes also suggests that
Asp38 could play an important role in cofactor specificity . -
pubmed
The
steroid receptor level was assayed by hormone binding techniques and specific mRNAs analyzed by solution hybridization using
35S - labelled RNA probes complementary to the ligand - binding domain of the
ER receptor gene and a 160 bp
PanI - Pvul fragment of the
IGF - I gene . -
pubmed
One hour after a single injection of
oestradiol ( 1 micrograms / kg BW ) , the
nuclear oestrogen receptor (
ERn ) mean level was increased 3 - fold whereas the
ER mRNA content had not changed significantly . -
pubmed
Cytochrome P450IA1 mRNA was induced in colon following a single oral administration of
I3C or
5,6BF . -
pubmed
Both inducers caused an approximately 2 - fold increase in
P450IA1 mRNA at 4 h and a 10 - fold increase at 16 h. In contrast , both
cytochrome P450IA1 and
IA2 mRNAs was increased over the control between 4 and 24 h. The total amount of
P450IA mRNAs in liver at 4 and 16 h was increased about 2- and 4 - fold respectively by
I3C ;
5,6BF induced the
P450IA mRNAs 4- and 5 - fold respectively . -
pubmed
1 -( 5 - Isoquinolinesulfonyl )- 2 - methylpiperazine , a putative inhibitor of
protein kinase C , does not modify the respiratory response to SP . -
pubmed
Rostrally , the heavily labeled
enkephalin areas coincide with areas strongly immunostained for
calcium - binding protein and with lightly stained areas in the
dopamine and
substance P immunoreactivity patterns . -
pubmed
In the same region lightly stained areas in the
enkephalin distribution match heavily labeled
substance P areas . -
pubmed
The moderately labeled
enkephalin areas caudomedially in the nucleus accumbens are in register with the heavily labeled regions in the distribution of
substance P and with the extremely heavily labeled regions in the distribution of
dopamine . -
pubmed
Altered
ATP function of a
vesicular stomatitis virus mutant detected by kinetic analysis of the
transcriptase using phosphorylated
ribavirin . -
pubmed
D2 agonists lowered the binding of
[ 3H ] raclopride to
D2 receptors in striatal and anterior pituitary tissues . -
pubmed
Pretreating the tissue with the
D1 - selective antagonist
SCH 23390 prevented the agonist - induced decrease in
[ 3H ] raclopride binding to
D2 sites in the striatum but not in the anterior pituitary , which has no
D1 receptors . -
pubmed
Conversely , a
dopamine - induced reduction in the binding of
[ 3H ] SCH 23390 to
D1 receptors could be prevented by the
D2 - selective antagonist
eticlopride . -
pubmed
The blocking effect by
SCH 23390 was similar to that produced by a nonhydrolyzable
guanine nucleotide analogue , and
SCH 23390 reduced the number of agonist - labeled
D2 receptors in the high - affinity state . -
pubmed
The effect of SK&
F 94482 (
BMY-25368 ) on 24 - hour intragastric acidity and
plasma gastrin concentration in healthy subjects . -
pubmed
There was also a sustained and significant 80 % rise in median 24 - h integrated
plasma - gastrin concentration during dosing with SK&
F 94482 ( 364 pmol h / L ) when compared with placebo ( 202 pmol h / L ; P = 0.003 ) . -
pubmed
The HAPE fluids also contained detectable amounts of
leukotriene B4 and other
lipoxygenase products of
arachidonic acid metabolism ,
complement fragments (
C5a ) , inhibitors of neutrophil chemotaxis , and
acid proteases but not
hydroxyproline , a constituent of
collagen . -
pubmed
It had reduced electrophoretic mobility , reacted normally with specific
albumin antiserum and , in contrast to normal
albumin , did not bind
nickel . -
pubmed
Sequence analysis showed that the minor band contained components with
Ala - His - Lys- and
His - Lys- N - terminal sequences , indicating removal of
Asp and
Asp - Ala respectively from the parent
albumin which was found to have the expected N - terminal sequence of
Asp - Ala - His - Lys . -
pubmed
Both normal
albumin and the minor component had an average of 0.32 residues of
glucose bound as
fructosamine . -
pubmed
The role of
arginyl residues in
porphyrin binding to
ferrochelatase . -
pubmed
The data obtained show that modification of
arginyl , but not
lysyl , residues results in the rapid inactivation of
ferrochelatase . -
pubmed
Reaction with
radiolabeled phenylglyoxal shows that modification of 1
arginyl residue causes maximum inhibition of
enzyme activity . -
pubmed
Comparison of the
enzyme kinetics for modified versus unmodified
enzyme show that modification with
camphorquinone-10 - sulfonic acid has no effect on the Km for
iron but does alter the Km for
porphyrin . -
pubmed
MPRP contains 115
amino acid residues including
phosphorylated serine at residues 1 , 2 , 6 , 12 , and 15 , and 6
O - glycosidic carbohydrate units at residues 69 , 75 , 87 (
threonine ) and 96 , 103 , and 106 (
serine ) . -
pubmed
MPRP has 5
phosphoserines ,
PRPs have 2 . -
pubmed
MPRP inhibits the spontaneous precipitation ( primary precipitation ) of
calcium phosphate salts from supersaturated solutions in addition to inhibiting seeded crystal growth ( secondary precipitation ) ( Oppenheim , F. G. , Offner , G. D. , and Troxler , R. F. ( 1982 ) J. Biol . -
pubmed
Synthesis of a copolymer containing
adenylic and deoxyadenylic acid residues with
polynucleotide phosphorylase . -
pubmed
ALA - dehydratase activation by
zinc . -
pubmed
The responses to
2 - chloroadenosine were affected to a lesser extent than were those to the other purines.6
Adenosine transport inhibitors ,
dipyridamole and
dilazep , potentiated responses to
adenosine , did not affect those to
NCPCA ,
NECA ,
L - PIA and
D - PIA but significantly reduced the responses to high doses of
2 - chloroadenosine.7 Relaxations evoked by
9 - beta - D - xylofuranosyladenosine which can activate intracellular but not extracellular
adenosine receptors , were attenuated by
dipyridamole but unaffected by
8 - phenyltheophylline.8 The results support the existence of an extracellular
A ( 2 )/ R ( a ) subtype of adenosine receptor and an intracellular
purine - sensitive site , both of which mediate relaxation . -
pubmed
Concentrations of 0.5 mM
acetaldehyde and 0.1 U / ml
xanthine oxidase caused reversible dilations , whereas higher concentrations produced initial constrictions followed by reversible dilations . -
pubmed
The following free radical scavengers inhibited the dilation when added to the lower concentrations of reactants :
superoxide dismutase , a
superoxide scavenger ;
catalase , an
H2O2 scavenger ; and
mannitol , a
hydroxyl scavenger . -
pubmed
The data support the hypothesis that
hydroxyl radicals can dilate pial arterioles , since all the scavengers can ultimately reduce levels of
hydroxyl generated by
acetaldehyde plus
xanthine oxidase . -
pubmed
Among the neurotransmitter bindings tested , only those of
GABA and
benzodiazepine in the cerebellum and beta - adrenergic ligands in the cerebral cortex are enhanced upon stimulation of
phospholipid methyltransferase activity . -
pubmed
Subsequent reduction of arterial pressure was observed with
prazosin hydrochloride , an
alpha - receptor antagonist . -
pubmed
In the presence of vascular
beta 2 - receptor blockade by
propranolol , the
hypertensive action of
epinephrine was mediated by
vascular alpha - receptors . -
pubmed
Experiments that employed labeled
FSH , reduced exposure time to purified
FSH , or decreased
calcium concentration in the medium proved that the increased
FSH secretory rate was not due to contamination of the medium with the purified
FSH used to stimulate the pituitary . -
pubmed
Estradiol-17 beta concentrations were always greater on the day of the coitus - induced ovulatory
LH surge ( mean = 51 + /- 5 pg / ml ) as compared to the previous day ( mean = 34 + /- 4 pg / ml ) when coitus did not result in a significant
LH release . -
pubmed
These data suggest that the ability to release
LH in response to coitus is in part a function of the duration of exposure to
estrogen by the hypothalamus and/or anterior pituitary . -
pubmed
Gel electrophoresis analysis indicates there is at least one intrachain
disulfide bond in
beta - dystroglycan . -
pubmed
Three - dimensional solution structure of the
calcium channel antagonist
omega - agatoxin IVA : consensus molecular folding of
calcium channel blockers . -
pubmed
The three - dimensional solution structure of
omega - agatoxin IVA , which is a specific blocker of the
P - type calcium channel isolated from funnel web spider venom and has a molecular mass of 5.2 kDa , was determined by two dimensional
1H NMR spectroscopy , combined with simulated annealing calculations . -
pubmed
We report a patient who developed Philadelphia chromosome negative
acute myeloblastic leukaemia with
trisomy 8 and
trisomy 11 after receiving treatment with
alkylating agents and
interferon for
chronic myelocytic leukaemia positive for Philadelphia chromosome . -
pubmed
We conclude that the leukaemic cells negative for Philadelphia chromosome may have developed as a result of treatment with
alkylating agents and
interferon in the present case . -
pubmed
Reduced dose
PVB treatment of stage IIC -
IV testicular seminoma . -
pubmed
Despite the information on the possible
cancer hazard among
SS - welders , current epidemiological data on
cancer incidence and mortality do not offer clear evidence to determine whether exposure to
nickel or
CrVI compounds constitutes the more important risk factor for
lung cancer . -
pubmed
2 - and
4 - fluorinated 16 alpha (-)[ 125I ] iodoestradiol derivatives : synthesis and effect on
estrogen receptor binding and receptor - mediated target tissue uptake . -
pubmed
The effect of
2 - and 4 - fluoro substitution on the
estrogen receptor - mediated tissue localization of
radioiodinated 16 alpha - iodoestradiol (
16 alpha - IE2 ) and its
11 beta - methoxy analogue (
11 beta - OMe-16 alpha - IE2 ) was evaluated . -
pubmed
The
4 - F analogue exhibited higher affinity for
estrogen receptors than the corresponding
2 - F analogue , and these differences were more pronounced at higher incubation temperatures . -
pubmed
Data in six components (
bile acids , esterified
cholesterol in whole plasma , and free
cholesterol in blood cells , bile ,
alpha lipoproteins , and
beta lipoproteins ) were modeled simultaneously with the SAAM program . -
pubmed
The analysis revealed extensive exchange of free
cholesterol between
HDL and liver , blood cells , and other tissues . -
pubmed
There was net free
cholesterol transport from
HDL to the liver in most subjects . -
pubmed
A large portion ( 4,211 mumol ) of total hepatic
cholesterol comprised a pool that turned over rapidly ( t1/2 of 72 min ) by exchanging mainly with plasma
HDL and was the major source of
bile acids and biliary
cholesterol . -
pubmed
HDL plays a central role in free
cholesterol exchange / transport between all tissues and plasma . -
pubmed
As well , the relative potency of
salicylic acid (
SA ) as compared with
ASA for these reproductive effects has not been well investigated . -
pubmed
The data failed to demonstrate a substantial potency difference between
ASA and
SA but some differences in toxicity were observed . -
pubmed
Both the highest administered dose of
SA and
ASA treatment contributed to increased maternal
peripartum death . -
pubmed
Lungs treated immediately before reperfusion with
catalase ( 5,000 units ) ,
desferrioxamine ( 2 mg / kg ) ,
NG - nitro - L - arginine methyl ester ( 5 mmol / l ) or saline showed a significant augmentation in pre -
ischaemia - reperfusion
hypoxic pulmonary vasoconstriction ( 57.7 + /- 6.0 % , 82.7 + /- 28.8 % , 95.2 + /- 36.6 % and 45.95 + /- 10.53 % respectively ) , an increase in
albumin flux ( 0.35 + /- 0.04 , 0.31 + /- 0.06 , 0.29 + /- 0.04 and 0.33 + /- 0.02 ) and an increase in pre -
ischaemia - reperfusion
palmitic :
linoleic acid ratio ( 0.64 + /- 0.08 , 0.51 + /- 0.19 , 0.5 + /- 0.04 and 0.17 + /- 0.07 ) . -
pubmed
administered immediately before reperfusion prevented completely the changes in hypoxic pulmonary vasoconstriction ( -0.3 + /- 5.4 % ) ,
albumin flux ( 0.09 + /- 0.11 ) and
palmitic :
linoleic acid ratio ( -0.06 + /- 0.12 ) . -
pubmed
In control lungs ( 2h of continuous perfusion ) ,
superoxide dismutase ,
catalase ,
desferrioxamine and saline did not affect
hypoxic pulmonary vasoconstriction ( 5.5 + /- 4.9 % , 1.0 + /- 3.1 % , -5.1 + /- 1.8 % and 3.0 + /- 6.6 % ) . -
pubmed
There was no effect of
superoxide dismutase ,
catalase ,
desferrioxamine ,
NG - nitro - L - arginine methyl ester or saline in control lungs on
albumin flux ( 0.10 + /- 0.04 , 0.11 + /- 0.01 , 0.1 + /- 0.01 , 0.12 + /- 0.01 and 0.11 + /- 0.01 respectively ) or
palmitic :
linoleic acid ratio ( -0.08 + /- 0.08 , 0.73 + /- 0.76 , -0.03 + /- 0.12 , 0.01 + /- 0.17 and 0.00 + /- 0.0 respectively ) . -
pubmed
Flavocytochrome b558 of the
NADPH oxidase which generates
superoxide in phagocytic cells , is a alpha1 beta1 heterodimer of
gp91phox and
p22phox , which together form a membrane - spanning electron - transport chain that transfers electrons from
NADPH in the cytosol to
oxygen . -
pubmed
The results indicated that while a temporary immunosuppression with
CsA achieved an indefinite islet allograft survival ,
FA administered to recipients daily was not able to prevent islet allograft rejection across a major histocompatibility barrier . -
pubmed
It is shown that mechanisms of
reperfusion fibrillations (
RF ) are more sensitive than ones of
RTS to changes of the intracellular
calcium concentration . -
pubmed
For example , RF have been easily suppressed by
calcium concentration decreasing at any stage of the experiment : upon reperfusion , or during previous
ischemia , whereas
RTS have not been suppressed even under total removal of
calcium from solutions either before initiation of
ischemia or during following reperfusion . -
pubmed
The analysis of RNAs from the virus - infected and
PAP - treated protoplasts demonstrated that 25S rRNA was depurinated by
PAP in the infected cells . -
pubmed
These responses were blocked by inhibition of
tyrosine kinase activity with
genistein , suggesting that the increases in
Ca2 + and
tyrosine phosphorylation are functionally coupled . -
pubmed
The transient was inhibited 25 - 40 % by
L - type Ca2 + channel antagonists and inhibited 90 - 95 % by
genistein . -
pubmed
The sustained response was unaffected by
L - channel antagonists and only slightly inhibited by
genistein . -
pubmed
The influx pathway during the transient probably involves an L - type
Ca2 + channel that is regulated by
tyrosine kinase activity . -
pubmed
The pathways that participate in the sustained response are different because they are insensitive to
l - channel antagonists and only slightly inhibited by
genistein . -
pubmed
Therefore ,
thapsigargin - mediated suppression of
serotonin - activated release probably reflects depletion of
Ca2 + from the sarcoplasmic reticulum , whereas
genistein - mediated suppression probably reflects inhibition of
tyrosine kinase linked release . -
pubmed
In the inner membrane of liver mitochondria , three major
45Ca2 + -binding polypeptides : a protein of approximately 130 kDa (
carbamoyl - phosphate synthetase ) , a glycoprotein of 43 - 44 kDa ( previously considered as the
calcium uniporter ) , and 29 - 30 kDa protein were found . -
pubmed
One of them might be the natural
Ca2 + -binding inhibitor of
H +- ATPase . -
pubmed
DNA adduct levels in lymphocytes were determined by the highly sensitive
nuclease P1 - enhanced
32P - postlabeling assay . -
pubmed
Hydrolytic cleavage of CAV to
canaline (
CAN ) and
urea is mediated by
arginase . -
pubmed
Modulation of
GT-1 DNA - binding activity by
calcium - dependent phosphorylation . -
pubmed
Taken together , our results suggest that
GT-1 may act as a molecular switch modulated by
calcium - dependent phosphorylation and dephosphorylation in response to light signals . -
pubmed
Serum
Zn ( 2 +)-stimulated
sphingomyelinase deficiency in type B
Niemann - Pick disease . -
pubmed
Influence of a
lysine 331 counterion on the pK(a ) of
aspartic acid 125 : evidence for a salt - bridge interaction and role in
alpha ( 1b )- adrenergic receptor activation . -
pubmed
The
amino acids , K331 and D125 , which comprise this salt - bridge , should be closely associated with each other in the unbound form of the
alpha ( 1b )- AR . -
pubmed
If the charged state of D125 is important for agonist binding , then changing the type of
amino acid at position 331 should decrease the acid strength of D125 , leading to
epinephrine affinity changes for the
alpha ( 1b )- AR . -
pubmed
For all mutations of the
alpha ( 1b )- AR where the positive charge at position 331 was eliminated , there was a significant increase in the pK(a ) ( congruent with 0.73 ) of an acidic
amino acid(s ) . -
pubmed
In this model , the binding capacity of lignin was equal to hemicellulose for PER ,
AZM and
CLP , but it was significantly higher for CKL . -
pubmed
Hemicellulose bound more
CKL ,
AZM and
CLP than did cellulose . -
pubmed
In addition , the effect on
aromatase expression of
charcoal - stripped fetal calf serum , of
quercetin and
genistein and of
tamoxifen and
raloxifene was investigated in both cell lines . -
pubmed
Cell
death is significantly blocked by the
caspase inhibitor
zVAD - fmk and when coexpressed with
E1B19 K ,
Bcl-2 or the
retinoblastoma protein (
RB ) . -
pubmed
Activation of
MAP kinases requires phosphorylation of both
Thr and
Tyr in the catalytic domain by a family of dual - specificity kinases , called
MEKs (
MAP kinase /
ERK kinase ) . -
pubmed
MEK1 is activated by phosphorylation at
Ser218 and
Ser222 by
Raf . -
pubmed
Mutation of these two sites to acidic residues , specifically [
Asp218 ] , [
Asp218 ,
Asp222 ] , and [
Glu218 ,
Glu222 ] , results in constitutively active
MEK1 . -
pubmed
PTX3 is a prototypic long
pentraxin consisting of a C terminal 203 -
amino acid pentraxin - like domain coupled with an N - terminal 178 -
amino acid unrelated portion . -
pubmed
In part these effects may involve a
nucleotide - induced release of
adenosine and the simultaneous activation of
P1 and
P2 receptors . -
pubmed
A membrane - bound
glucose dehydrogenase quinoenzyme [ which contains
pyrroloquinoline quinone (
PQQ ) as the prosthetic group ] is involved in that oxidation . -
pubmed
diazotrophicus
PAL3 was grown in a chemostat with atmospheric
nitrogen as the sole N source provided that the dissolved
oxygen was maintained at 1.0 - 2.0 % air saturation . -
pubmed
Here , the effects of
db - cAMP in lung preservation were ultrastructurally investigated , and the ultrastructural changes before reperfusion were correlated with
pulmonary function after reperfusion . -
pubmed
In the fresh group ( n = 6 ) , the lungs were flushed with extracellular - type
trehalose - containing (
ET - K ) solution and were reperfused immediately . -
pubmed
In the control group ( n = 6 ) and db -
cAMP group ( n = 5 ) , the lungs were flushed with
ET - K solution and
ET - K solution plus
db - cAMP ( 2 mM ) , respectively , and were reperfused after cold preservation at 4 degrees C for 15 h. Before reperfusion , tissue was sampled and ultrastructurally analyzed by transmission electron microscopy . -
pubmed
Dosing preparations included uncooked fresh sprouts ( with active
myrosinase ) as well as homogenates of boiled sprouts that were devoid of
myrosinase activity and contained either
glucosinolates only or
isothiocyanates only . -
pubmed
Essential role of
NHE3 in facilitating
formate - dependent
NaCl absorption in the proximal tubule . -
pubmed
The virtually complete dependence of
formate - induced
NaCl absorption on
NHE3 activity raises the possibility that
NHE3 and the
formate transporters are functionally coupled in the brush border membrane . -
pubmed
dose - dependently suppressed neutrophil infiltration and
nitric oxide ( but not
KC and
IL-1beta ) production in
peritonitis . -
pubmed
In a model of systemic
endotoxemia ,
PJ34 pretreatment significantly reduced plasma levels of
TNF - alpha ,
IL-1beta and
nitrite /
nitrate ( breakdown products of
nitric oxide ) production . -
pubmed
Delaying the start of
PJ34 administration in the
colitis model conferred significant protective effects , while in the
arthritis model the
post - treatment paradigm lacked protective effects . -
pubmed
A series of novel synthetic derivatives , comprising
monohydroxylated benzoperimidines and
2 - aminobenzoperimidines , allowed the establishment of an in vitro structure - activity relationship for a panel of
leukemia sensitive , as well as
P - gp dependent multidrug resistance ( MDR ) and multidrug resistance associated protein dependent resistance (
MRP ) resistant cell lines . -
pubmed
The aim of this study was to investigate the role of
inducible nitric oxide ( NO ) synthase (
iNOS ) and
NO on the modulation of the inflammatory response caused by splanchnic
ischemia and reperfusion . -
pubmed
These results suggest that the induction of
iNOS and
NO production are essential for the upregulation of the inflammatory response in splanchnic
ischemia / reperfusion and participate in end organ damage under these conditions . -
pubmed
Combinations using an
alpha2 - adrenoceptor agonist and
ketamine have been shown to be effective for immobilization but are not reversible and can produce severe
hypertension and prolonged or rough recoveries . -
pubmed
We assessed the effect of endogenous
GABA on ictal activity and neuronal firing rate in hippocampal slices from postnatal day 1 (
P1 ) to P30 . -
pubmed
In extracellular recordings , there was a strong correlation between the postnatal age at which
GABA ( A )- R antagonists decreased action potential frequency (
P23 ) and the age at which ictal activity could be induced by elevated
potassium (
P23 ) . -
pubmed
In addition , there was a strong correlation between the fraction of slices in which ictal activity was induced by elevated
potassium concentrations and the fractional decrease in action potential firing when
GABA(A)-Rs were blocked in the presence of
ionotropic glutamate receptor antagonists . -
pubmed
Finally , ictal activity induced by elevated
potassium was blocked by the
GABA ( A )- R antagonists
bicuculline and
SR-95531 (
gabazine ) and increased in frequency and duration by
GABA ( A )- R agonists
isoguvacine and
muscimol . -
pubmed
In bipolar cells , the number of
Ca entry sites and
ribeye - positive fluorescent
spots is also strikingly similar to that of exocytic active zones but significantly less than the number of total exocytic sites including solitary fusion events outside active zones . -
pubmed
[
Mg ( 2 +)]( i ) was increased after the stimulation with 100 microU / mL of
insulin . -
pubmed
After 60 seconds of
insulin stimulation , the value of [
Mg ( 2 +)]( i ) was lower in the type 1
DM group compared with the control group ( 729 + /- 85 micromol / L v 1,078 + /- 67 micromol / L , P & lt;.005 ) . -
pubmed
The activity of
cytochrome oxidase (
COX ) , coupled to
ATP production , reflects long - term plasticity in metabolic capacity . -
pubmed
TUDCA inhibition of
cholangiocarcinoma growth was blocked by
BAPTA - AM , an intracellular
Ca ( 2 + ) concentration ( [
Ca ( 2 +)]( i ) ) chelator , and H7 , a
PKC - alpha inhibitor . -
pubmed
TUDCA inhibited the activity of
MAPK , and this inhibitory effect of
TUDCA was abrogated by
BAPTA - AM and H7 . -
pubmed
TUDCA did not alter the activity of
Raf-1 and
B - Raf and the phosphorylation of
MAPK p38 and
JNK /
stress - activated protein kinase . -
pubmed
TUDCA inhibits Mz - ChA-1 growth through a signal - transduction pathway involving
MAPK p42 / 44 and
PKC - alpha but independent from
Raf proteins and
MAPK p38 and
JNK /
stress - activated protein kinases . -
pubmed
A number of drugs are used in
neurodegenerative disorders including antidepressants ,
benzodiazepines , antipsychotics ,
acetylcholinesterase inhibitors and
levodopa . -
pubmed
Co - administration of
monoamine oxidase inhibitors and
opioids should be avoided as it can cause
agitation ,
muscular rigidity ,
sweating and
hyperpyrexia . -
pubmed
Four broad groups of investigational therapeutic agents appear promising for future therapy : modified
interferons and
ribavirins , immunomodulators , viral life - cycle targets , and antifibrotic agents . -
pubmed
Heme oxygenase , steering
dioxygen activation toward
heme hydroxylation . -
pubmed
The activation of
dioxygen by
heme oxygenase proceeds via formation of an obligatory
ferric hydroperoxide intermediate (
FeIII - OOH ) , as is the case in the activation of
dioxygen by
monooxygenase enzymes . -
pubmed
This review summarizes current understanding of the structural and dynamic properties in
heme oxygenase that channel the reactivity of the
FeIII - OOH intermediate toward
heme hydroxylation rather than
oxoferryl formation . -
pubmed
Differences in mechanism between
heme hydroxylation , as carried out by
heme oxygenase , and the coupled oxidation process , are also addressed . -
pubmed
Photochromic agents as tools for protein structure study :
lapachenole is a photoaffinity ligand of
cytochrome P450 3A4 . -
pubmed
These attributes of the
chromenes inspired testing of their potential to label
cytochrome P450 3A4 in a light - dependent fashion . -
pubmed
Access to the
protein active site by
lapachenole was demonstrated with the molecule 's ability to competitively inhibit
P450 3A4 - mediated oxidative metabolism of
midazolam with an IC(50 ) value of 71 microM. -
pubmed
These results clearly demonstrate that
chromenes are effective photoaffinity reagents for the
cytochrome P450 superfamily of
enzymes and probably other proteins as well . -
pubmed
Kahalalide F induces
necrosis - like cell
death that involves depletion of
ErbB3 and inhibition of
Akt signaling . -
pubmed
Kahalalide F (
KF ) is a novel marine - derived antitumor agent that is currently undergoing phase II clinical trials . -
pubmed
Several markers of
caspase - dependent apoptosis , such as
phosphatidyl - serine externalization ,
cytochrome c release , and
caspase-3 and
poly -( ADP - ribose ) polymerase cleavage were negative after
KF exposure . -
pubmed
EPA505 and related
strains in
PAH - contaminated soils , a new
16S rRNA gene - based primer set was designed . -
pubmed
Applying the new primer set ,
16S rRNA gene fragments which were 99 - 100 % similar to the corresponding gene of
strain EPA505 were amplified from four of five
PAH - contaminated soils . -
pubmed
The proteins bound on the interacting RNA are eluted and subjected to
SDS - PAGE , and the Coomassie - stained protein bands are cut and subjected to mass spectrometry (
MS ) analysis . -
pubmed
Exogenous NE ( 3 micromol / l ) ,
alpha , beta - methylene ATP ( 1 micromol / l ) ,
neuropeptide Y (
NPY , 0.1 micromol / l ) ,
CGRP ( 0.1 micromol / l ) ,
vasoactive intestinal peptide (
VIP , 0.1 micromol / l ) , and
substance P ( SP , 0.1 micromol / l ) had no effect on the basal release of
beta - NAD , suggesting that the overflow of
beta - NAD is evoked by neither the sympathetic neurotransmitters NE ,
ATP , and
NPY , nor the neuropeptides
CGRP , VIP , and
SP . -
pubmed
Botulinum neurotoxin A ( BoNTA , 0.1 micromol / l ) abolished the evoked release of NE ,
ATP , and
beta - NAD at 4 Hz , suggesting that at low levels of neural activity , release of these neurotransmitters results from
N - ethylmaleimide - sensitive factor attachment protein receptor / synaptosomal - associated protein of 25 kDa - mediated exocytosis . -
pubmed
Immune reconstitution was noted to occur at a significantly faster rate in the secondary recipients , and without the occurrence of
graft - versus - host disease (
GVHD ) , despite the presence of donor cells mismatched for 1 to 3
HLA antigens . -
pubmed
Nanosensor - expressing plants can be used to assess
glucose flux differences between cells ,
invertase - mediated
sucrose hydrolysis in vivo , delivery of assimilates to roots , and
glucose flux in mutants affected in
sugar transport , metabolism , and signaling . -
pubmed
[ Labeling of
CDTPA - dianhydride - coupled
CD45 monoclonal antibody with
yttrium-90 ] . -
pubmed
OBJECTIVE : To explore the methods for labeling CDTPA - coupled
CD45 monoclonal antibody ( mAb ) with
yttrium-90 (
( 90 ) Y ) for potential acute myeloid therapy . -
pubmed
METHODS :
CD45 mAb was labeled with
( 90 ) Y by
CDTPA and the labeling rate , radiochemical purity , final specific activity , and immunological activity of the mAb were detected . -
pubmed
CONCLUSION :
( 90 ) Y - CDTPA -
CD45 mAb possesses good properties as an ideal targetting therapeutic agent for
acute leukemia . -
pubmed
This is due to the distinct content of the X and Y - chromosomes in both genes and regulatory sequences ,
SRY being the key regulator Hormones (
AMH ,
testosterone ,
Insl3 ) secreted by the foetal testis ( gonadal sexual dimorphism ) , impede Muller duct development , masculinize Wolff duct derivatives and are involved in testicular descent ( hormonal sexual dimorphism ) . -
pubmed
In addition , the correlation of both
carbohydrate antigens expression was evaluated with microvessel density (
MVD ) . -
pubmed
RESULTS : Expressions of
sialyl Lewis antigens and
MVD were associated with several clinicopathological features that reflect the
tumor aggressiveness in
esophageal cancer . -
pubmed
Essential role of
protein kinase C zeta in transducing a motility signal induced by
superoxide and a chemotactic peptide , fMLP . -
pubmed
Amino acid homology to other
LFYs is 43 - 55 % ; conserved and variable regions were similar to others . -
pubmed
Known features of
transcription factors exhibited by
ScLFY were an acidic domain in the central variable region , a
proline - rich variable - region , and glutamate - rich regions . -
pubmed
Independent predictors of drug resistance were the earlier calendar year , prior use of suboptimal
nucleoside analogue therapy , male sex and higher
CD4 levels at testing . -
pubmed
Cd and
Pb(
EDTA ) produce the strongest increases of
OASTL activity , with the higher enhancement seen in roots , in parallel with the higher metal accumulation . -
pubmed
Arsenic produced an increase of root
enzyme activity , whereas
Cu produced a decrease , mainly in shoots . -
pubmed
OASTL transcripts were increased by
As ,
Cd , and
Pb , especially in roots , where metal accumulation was maximal , while
Cu produced a decrease in the transcript levels . -
pubmed
More than 20 % of all subjects were using their inhaler device incorrectly (
Turbuhaler 37.1 % ; Metered - dose inhaler 28.1 % ;
Diskus 21.3 % ; Aerolizer 7.4 % ) and this was associated with a 0.84 - point increase in
ACS . -
pubmed
Exercise - induced protection against myocardial apoptosis and
necrosis :
MnSOD ,
calcium - handling proteins , and calpain . -
pubmed
We hypothesized that an exercise - induced increase in
MnSOD would provide cardioprotection by attenuating IR - induced oxidative modification to critical
Ca ( 2 +)-handling proteins , thereby decreasing calpain - mediated cleavage of these and other proteins attenuating cardiomyocyte
death . -
pubmed
In addition , exercise prevented IR - induced calpain activation as well as the oxidative modification and calpain - mediated degradation of myocardial
Ca ( 2 +)-handling proteins (
L - type Ca ( 2 + ) channels ,
phospholamban , and
sarcoplasmic / endoplasmic reticulum calcium ATPase ) . -
pubmed
These results suggest that
MnSOD provides cardioprotection by attenuating IR - induced oxidation and calpain - mediated degradation of myocardial
Ca ( 2 +)-handling proteins , thereby preventing myocardial apoptosis and
necrosis . -
pubmed
There are seven related
NADPH oxidases (
NOX 1 - 5 ,
DuoNOX 1,2 ) which can be activated in various ways to produce
superoxide and
hydrogen peroxide at the plasma membrane . -
pubmed
For this purpose , a
post hoc subanalysis of the randomized , placebo - controlled CAPRAF (
Candesartan in the Prevention of Relapsing
Atrial Fibrillation ) trial was performed . -
pubmed
OBJECTIVE : The purpose of our study was to use the total
choline - containing compound ( tCho ) peak integral as a marker of
malignancy in breast MR spectroscopy (
MRS ) . -
pubmed
Identification of
amino acid residues of the
pheromone - binding domain of the
transcription factor TraR that are required for positive control . -
pubmed
Here , we constructed 117 mutants , altering 103 surface - exposed
amino acid residues of the
TraR N - terminal domain . -
pubmed
Amino acid residues required for activation of both promoters probably contact the C - terminal domain of the
RNA polymerase alpha subunit , while residues required only for
traI promoter activation may contact another
RNA polymerase component . -
pubmed
For instance , the high -
zinc brass alloys ( with the highest levels of
tin and
zinc and the lowest melting temperature ) correspond to most of the Picasso sculptures , correlate with the Valsuani foundry , and are associated with the most recent sculptures (
post - WWII ) and with the lost - wax casting method . -
pubmed
The accumulative penetration of
DXM and AB within skin flap were also detected at 2 and 6 hours after application of 2 g of compound gel containing 3%AZ/2%PG ( group
A2 ) and peritoneal injection AB ( 2 mg / kg ) /
DXM ( 5 mg / kg ) ( group B2 ) . -
pubmed
After 2 and 6 hours , the quantities of
DXM and AB in skin flap of group
A2 were significantly higher than that of group B2 ( P & lt ; 0.05 ) . -
pubmed
Activity of the
multikinase inhibitor
sorafenib in combination with
cytarabine in
acute myeloid leukemia . -
pubmed
We investigated a treatment strategy combining
sorafenib , a
multikinase inhibitor with limited single - agent activity in
AML , and
cytarabine , a key component of
AML chemotherapy . -
pubmed
RESULTS :
Sorafenib at 10 muM inhibited cell viability , proliferation and
ERK1 / 2 signaling , and induced apoptosis in all cell lines studied . -
pubmed
In this work a protocol for the
enzyme - assisted removal of non - triple helical polypeptide entities in atelocollagen solutions was conceived , using
pronase as scavenger , in the presence of
poly ( ethyleneglycol ) (
PEG 10000 ) as a crowding agent ,
trimethylamine - N - oxide dihydrate , (
TMAO ) as kosmotropic agent and
anhydrous sodium sulfate as anti - chaotrope salt . -
pubmed
Total
cAMP response element binding protein (
CREB ) concentrations ranged from 0.8 ng / ml to 1 ng / ml , whereas the proportions of
phospho -
CREB versus total
CREB differed between the cell lines . -
pubmed
Basic fibroblast growth factor (
FGF-2 ) and
epidermal growth factor (
EGF ) stimulated
cAMP generation , whereas
leukaemia inhibiting factor reduced some of their effects . -
pubmed
Forskolin ( 0.05 and 1 mM ) acted in synergy with
FGF-2 and
EGF ; however , it caused pronounced donor to donor differences in the increase of
cAMP and
phospho -
CREB levels . -
pubmed
Heterogeneity of donor cell responses to stimulation of the
cAMP pathway indicates the need for wide safety margins for S -
MSC use in drug screening ; nevertheless , knowledge of this heterogeneity might be useful for the design of donor - specific cell therapy . -
pubmed
Salinomycin can effectively kill
ALDH(high ) stem - like cells on
gastric cancer . -
pubmed
Importantly ,
ALDH(high )
cancer cells are relatively sensitive to
salinomycin when compared to
ALDH(low )
cancer cells ( P<0.01 ) . -
pubmed
Hemoglobin (
Hb)-based
oxygen carriers ( HBOCs ) are being developed as resuscitative fluids for use in multiple medical applications and in lieu of blood transfusion . -
pubmed
We evaluated
thyroid hormone levels , ultrasound examination of thyroid and urine
iodine concentration ( UIE ) at each trimester and at 3 - month
post - partum period (
PP ) . -
pubmed
During weight recovery , OC and
CTX were independently and positively associated with weight ,
weight gain , IGF-1 and
oestradiol . -
pubmed
Lyophilisomes were prepared by a freezing , annealing , and lyophilization method and a
cystein - elongated
TAT peptide was conjugated to the lyophilisomes using a heterobifunctional linker . -
pubmed
Here we place the
FLT3 - ITD upstream of
BTK in
AML and show that the
BTK inhibitor
ibrutinib inhibits the survival and proliferation of
FLT3 - ITD primary
AML blasts and
AML cell lines . -
pubmed
Furthermore
ibrutinib inhibits the activation of downstream
kinases including
MAPK ,
AKT and
STAT5 . -
pubmed
Finally we report that
ibrutinib reverses the cyto - protective role of BMSC on
FLT3 - ITD
AML survival . -
pubmed
Progesterone concentration was not different ( P & gt ; 0.05 ) in
S1 compared to
S2 . -
pubmed
This report analyses the potential of
[ ( 68 ) Ga ] Glu - urea - Lys ( Ahx )- HBED - CC (
( 68 ) Ga - PSMA-11 ) , a new positron emission tomography ( PET ) tracer targeting
prostate - specific membrane antigen (
PSMA ) for
prostate cancer staging and individualized radiotherapy planning . -
pubmed
Staging results of conventional imaging , including bone scintigraphy ,
CT or MRI , were compared with
( 68 ) Ga - PSMA ligand PET /
CT results and the influence on radiotherapeutic management was quantified . -
pubmed
RESULTS :
( 68 ) Ga - PSMA ligand PET /
CT had a dramatic impact on radiotherapy application in the presented cohort . -
pubmed
Diabetic status was monitored using blood and urine , and at the end , harvested kidneys were used to study the amelioration of glycoprotiens (
collagen ) and HS by enzymatic digestion , spectrophotometric ,
hydroxyproline and
agarose electrophoretic methods . -
pubmed
Increased
collagen content in
diabetic group was observed by
hydroxyproline estimation ( 24 % ) and
periodic acid - Schiff 's ( PAS ) staining . -
pubmed
Results of
monosaccharide composition indicated that
CSAP and its neutral fractions mainly consisted of
mannose and
galactose ( 79.45 - 97.52 % ) with M / G ratios of 0.94 - 1.48 , typical for galactomannans , which were demonstrated by FT - IR . -
pubmed
Both
Shank3(e4 - 9 )
HET and KO demonstrated a significant reduction in
NMDA /
AMPA ratio at excitatory synapses onto striatal medium spiny neurons . -
pubmed
In
HSF-1(-/-)/
LDLr(-/- ) liver , higher
cholesterol 7alpha - hydroxylase (
CYP7A1 ) and
multidrug transporter [
MDR1 /
P - glycoprotein (
P - gp ) ] gene expressions were observed , consistent with higher
bile acid transport and larger hepatic bile ducts . -
pubmed
The molecular weight and the Michaelis constants were determined ; the effect of
hydrogen ions on the forward and reverse
MDH reaction was studied . -
pubmed
The results of the study demonstrated that the
enzyme consists of subunits ; the molecular weight of subunits was determined by
SDS - PAGE . -
pubmed
The model takes the synergistically acting light / dark cycles and feeding rhythms as inputs and incorporates the activity of
sirtuin 1 , a cellular energy sensor and a metabolic enzyme activated by
nicotinamide adenine dinucleotide . -
pubmed
Other abnormalities include high
renin ,
secondary hyperaldosteronism , and elevated levels of
prostaglandin E2 . -
pubmed
FMD was higher in the
isoflavone group than in the control group after 24 - week therapy ( treatment effect 2.0 % , 95 % CI 0.18 - 3.0 , P = 0.01 ) . -
pubmed
The baseline differences were adjusted in
FMD and the
isoflavone therapies were closely associated with the reduction of
FMD impairment at 24 weeks ( odds ratio 0.30 , 95 % CI 0.14 - 0.85 , P = 0.01 ) . -
pubmed
The effects of
isoflavone on brachial
FMD were negatively associated with base
FMD ( r = -0.65 , P & lt ; 0.01 ) . -
pubmed
Furthermore ,
isoflavone therapy caused a significant increase in the levels of
erythroid - derived 2 - like 2 (
Nrf2 ) ,
superoxide dismutase (
SOD ) and a significant decrease in serum levels of
C - reactive protein ,
8 - isoprostane ,
malondialdehyde ,
interleukin-6 and
tumor necrosis factor alpha . -
pubmed
In contrast , the
isoflavones had no effects on the levels of oxidation - related molecules when
Nrf2 was silenced . -
pubmed
PURPOSE : In the phase III EMILIA study ,
trastuzumab emtansine ( T -
DM1 ) significantly improved progression - free survival ( PFS ) and overall survival ( OS ) versus
capecitabine plus
lapatinib ( control ) in previously treated
human epidermal growth factor receptor 2 - positive advanced
breast cancer . -
pubmed
Plasma was analysed for
triglycerides ,
glucose ,
insulin , and non - esterified
fatty acids (
NEFA ) . -
pubmed
34.7 % of patients never took
vitamin D within 12 months after surgery , 26.2 % took
vitamin D inconsistently , and
39.1 % took
vitamin D consistently . -
pubmed
In this study , we have elucidated and verified the chemical structure of two major equine
arylpropionamide - based SARM metabolites using a combination of chemical synthesis and liquid chromatography - mass spectrometry ( LC -
MS ) analysis . -
pubmed
Cannabinoid - induced cell
death in
endometrial cancer cells : involvement of
TRPV1 receptors in apoptosis . -
pubmed
Finally , these effects were dependent on
TRPV1 activation and intracellular
calcium levels . -
pubmed
Selective targeting of
TPRV1 by
AEA ,
CBD , or other stable analogues may be an attractive research area for the treatment of
estrogen - dependent endometrial
carcinoma . -
pubmed
The WT
MRP4 molecular structure confirmed and ameliorated the general knowledge about the transmembrane helices and the two
nucleotide binding domains . -
pubmed
They were then randomly divided into 6 groups : control group ( I ) ,
chlorhexidine (
II ) , Khadiradi vati ( III ) , Dashansanskar churana (
IV ) , Neem ( V ) , and Apamarga ( VI ) ; based on the drugs used by patients for the maintenance of oral hygiene after oral prophylaxis . -
pubmed
PYT and some of its derivatives , including
phytanic acid (
PA ) , exert a wide range of biological effects . -
pubmed
PPARs- and
NF - kappaB - mediated activities are also discussed as mechanisms responsible for some of the bioactivities of
PYT . -
pubmed
Carbamazepine (
CBZ ) ,
oxcarbazepine (
OXC ) , and
eslicarbazepine acetate (
ESL ) belong to the
dibenzazepine family of antiepileptic drugs and are all thought to primarily act as
sodium channel blockers ( SCBs ) . -
pubmed
Negative Impacts of Biochars on
Urease Activity : High pH , Heavy Metals ,
Polycyclic Aromatic Hydrocarbons , or Free Radicals ? -
pubmed
The results showed that biochars inhibited
urease activity ( indicated by the
NH4 + generation ) . -
pubmed
Released heavy metals and
polyaromatic hydrocarbons explained the 20 % inhibition of
urease activity in biochar supernatants ( 200 C ) but could not explain the 70 % inhibition in particle systems ( 400 C ) . -
pubmed
The levels of protein
carbonyl and
nitrotyrosine , common oxidative damagers of
urease , were high in particle systems but very low in biochar supernatants . -
pubmed
The observed inhibition of
urease activity may be the result of oxidative reactions with free radicals on the biochar surface or oxidative reactions with reactive
oxygen species promoted by free radicals . -
pubmed
This minireview summarizes what is currently known about the sources and fate of
TMA in marine environments and the different pathways and
enzymes involved in
TMA metabolism in marine bacteria . -
pubmed
The formation of 2a is initiated by the one - electron reduction of [ 1a]+ , followed by a reversible intramolecular rearrangement terminating with the irreversible release of
CpH ( NMR and gas chromatography - mass spectrometry ) and
Fe [ electron paramagnetic resonance (
EPR ) and magnetometry ] . -
pubmed
The key intermediate
iron ( I ) ferraferrocene ( 3 ) was detected by
EPR and IR spectroelectrochemistry , while the related species 3 - H-3 was isolated after the addition of a
hydrogen source and then identified by X - ray diffraction . -
pubmed
The functionalized
diiron compounds 4 ( 52 % yield ) and 5 ( 62 % ) were afforded through the activation of
O2 and
S8 by a radical intermediate along the reductive pathway of [ 1a]+ . -
pubmed
Rapid Layer Technology ( RLT ) uses computer - aided design / computer - aided manufacturing (
CAD / CAM ) to manufacture a veneer layer that is adhesively bonded to the
zirconia framework , avoiding firing steps during the fabrication process and thus preventing build - up of residual stresses . -
pubmed
For the latter , the veneers were
CAD / CAM fabricated using a feldspathic reinforced - glass ( Vitablocs Mark
II ; n = 16 ) or a polymer - infiltrated reinforced - glass network ( Enamic ; n = 16 ) and adhesively bonded to the
zirconia frameworks . -
pubmed
Recent findings have identified
salmon calcitonin (
sCT ) , an
amylin receptor agonist and analogue of endogenous
amylin , as a potential regulator of
alcohol - induced activation of the mesolimbic
dopamine system and
alcohol consumption . -
pubmed
Providing that the role of
amylin signalling in
alcohol - related behaviours remains unknown , the present experiments investigate the effect of
sCT on these behaviours and the mechanisms involved . -
pubmed
Collectively , our data suggest that
amylin signalling , especially in the NAc , may contribute to reduction of various
alcohol - related behaviours . -
pubmed
Correlation analysis corrected for the presence of confounding variables (
riluzole , age at onset , site of onset , gender ) was tested between
PRL ( Progression Rate at the Last visit ) and LMVs , and a statistically significant value was found ( Pearson partial correlation r = 0.407 , p = 0.006 ) . -
pubmed
Activated
PPARs participate in the regulation of lipid and
glucose metabolism and also affect cellular proliferation , differentiation , and apoptosis , and the immune responses . -
pubmed
Also , the serum levels of fasting blood
sugar ( P & lt ; 0.001 ) , HbA1c ( P = 0.04 ) ,
cholesterol ( P = 0.03 ) ,
triglycerides ( P = 0.01 ) , blood
urea nitrogen ( P = 0.03 ) , and pro - inflammatory
cytokines including
IL-1beta ,
IL-6 , and
TNF - alpha were significantly higher in BN candidates for LSG ( P & lt;= 0.001 ) . -
pubmed
Efficacy and safety of
TNF blockers and of
ustekinumab in
palmoplantar pustulosis and in
acrodermatitis continua of Hallopeau . -
pubmed
OBJECTIVES : To evaluate the real - life efficacy of
tumor necrosis factor blockers and
ustekinumab in
PPP and in
ACH . -
pubmed
CONCLUSIONS : We found no difference in efficacy between
TNF blockers and
ustekinumab and among the three different
TNF blockers in real life for
PPP or ACH , which reveals the heterogeneity of clinical response to biologics in
pustular psoriasis as compared with
plaque - psoriasis . -
pubmed
In vitro effect of some
nucleoside reverse transcriptase inhibitors against HSV-1 replication . -
pubmed
OBJECTIVE : The current study was designed to investigate the effects of some
nucleoside reverse transcriptase inhibitors ( NRTIs ) on
HSV-1 infection . -
pubmed
MATERIALS AND METHODS : Initially , the SwissTargetPrediction server was used to predict the interactions between
HSV-1 thymidine kinase and
acyclovir ,
stavudine ,
zidovudine ,
didanosine , and
entecavir . -
pubmed
Transmission
electron microscopy could detect no disruption of normal endocytosis at the apical surface of
Tmc1Delta;
Tmc2Delta hair cells in vitro . -
pubmed
Besides complement inhibition ,
C1 - INH also prevents
FXII activation , a very early event of contact phase activation at the crossroads of coagulation and
inflammation . -
pubmed
The analysis of various blood and plasma parameters revealed that
C1 - INH - containing coatings were able to markedly reduce
FXIIa activity compared to heparin coating . -
pubmed
Combined
C1 - INH / heparin coatings yielded similarly low levels of
thrombin -
antithrombin III complex formation as heparin coating . -
pubmed
Significant differences ( p < 0.05 ) were only found in the expression of
glutathione transferase , which was increased almost 3 times in the group treated with beta - glucans as compared with the control group , and 1.5 times as compared with the group treated with
acrylamide . -
pubmed
,
catalase (
CAT ) ,
peroxidase (
POD ) , and
super oxide dismutase (
SOD ) ) ,
malondialdehyde (
MDA ) , and
Pb concentration in plants subjected to different
Pb concentrations . -
pubmed
Si NPs minimized accumulation of
MDA in plant tissues and adjusted the activities of
POD ,
CAT , and
SOD in plants under
Pb stress . -
pubmed
Overall ,
Si NP foliar application might be a suitable approach in reducing the
Pb concentrations in plants . -
pubmed
Twelve healthy males ( stature : 1.75 + - 0.08 m ; body mass : 67.5 + -
6.3 kg ; age : 21.0 + - 1.4 years ; maximal
oxygen consumption : 45.1 + - 7.0 ml.kg.min-1 ) attended four separate laboratory visits . -
pubmed
Cycling TTE increased for
SBC2 ( +20.2 s ; p = 0.045 ) and SBC3 ( +31.9 s ; p = 0.004 ) compared to
PLA . -
pubmed
GI upset was exacerbated
post - exercise after SBC3 for
nausea compared to
SBC2 and PLA ( p < 0.05 ) , whilst
SBC2 was not significantly different to
PLA for any symptom ( p > 0.05 ) . -
pubmed
Biochemical methods were used ( RNA , DNA ,
total protein content , liver antioxidant activity ,
insulin and
glucose levels in the blood ) , transmission electron microscopy , morphometric analysis of mitochondria ( number , average and total area ) . -
pubmed
Regulation of mitochondrial and microsomal
phospholipid synthesis by
liver fatty acid - binding protein . -
pubmed
Recently we have detected and partially purified a 15 - kDa cytosolic
L - alpha - lysophosphatidic acid ( LPA )- binding protein (
LPABP ) , which stimulates export of
LPA from mitochondria ( Vancura , A. , Carroll , M. A. , and Haldar , D. ( 1991 ) Biochem . -
pubmed
By Western immunoblot analysis ,
amino acid sequence analysis , and binding characteristics we have shown that
LPABP is identical with
liver fatty acid - binding protein (
L - FABP ) . -
pubmed
This protein binds
LPA , and stimulates mitochondrial and microsomal
glycerophosphate acyltransferase (
GAT ) and the export of
LPA from both the organelles . -
pubmed
Taken together , these findings are suggestive that
L - FABP plays a major role in mitochondrial and microsomal phospholipid metabolism by regulating both the synthesis and utilization of
LPA . -
pubmed
Three analogues with restricted flexibility were designed to study the active conformation of
verapamil during interaction with the
slow calcium channel . -
pubmed
Interferon alpha and
etretinate combination treatment of
cutaneous T - cell lymphoma . -
pubmed
A birth control vaccine incorporating a synthetic peptide antigen representing the
aminoacid sequence 109 - 145 of the C - terminal region of the beta subunit of
human chorionic gonadotropin (
hCG - beta ) was submitted to a phase 1 clinical trial . -
pubmed
We conclude that the acutely preovulatory expression of
CA-125 in serum and
FF as determined by EIA is low and not significantly enhanced by
OH . -
pubmed
Serum aminoterminal propeptide of type III procollagen (
PIIINP ) was studied in 73
psoriatics receiving
methotrexate and in 11 selected for trial with
methotrexate or
etretinate . -
pubmed
PIIINP is not specific for the liver , but the study indicates that the number of liver biopsies can be reduced in
psoriatics on
methotrexate who have normal levels of
PIIINP . -
pubmed
Pharmacological activity of
N - methyl - carbamylcholine , a novel
acetylcholine receptor agonist with selectivity for
nicotinic receptors . -
pubmed
N - Methyl - carbamylcholine ( also called
N - methyl - carbachol ) is an analogue of the mixed
muscarinic - nicotinic acetylcholine receptor agonist ,
carbachol . -
pubmed
To determine whether
N - methyl - carbachol acts as an agonist or an antagonist at
nicotine and/or
muscarinic receptor sites , the present study examined the pharmacological activity of this compound on some cholinergically innervated tissues . -
pubmed
The results suggest that the
cytotoxicity ,
clastogenicity and
mutagenic activity of the
amsacrine analogues is mediated by similar mechanisms , probably involving the enzyme
DNA topoisomerase II . -
pubmed
Compared with the control group ,
lovastatin alone decreased the plasma total C value by 36 % ( p =
NS ) , the
LDL - C level by 35 % ( p =
NS ) , hepatic C content by 29 % ( p =
NS ) , and hepatic C synthesis by 52 % ( p =
NS ) . -
pubmed
Compared with the control group , the combination of PIB and
lovastatin decreased the plasma total C level by 78 % ( p less than 0.005 ) , the
LDL - C level by 74 % ( p less than 0.025 ) , and hepatic C content by 58 % ( p less than 0.05 ) ; however , the hepatic C synthesis increased by 490 % ( p less than 0.005 ) compared with the control group and by 110 % ( p less than 0.05 ) compared with PIB alone . -
pubmed
We conclude that both
PIB and
lovastatin lower plasma total C and
lipoprotein C fractions . -
pubmed
The polar head group that was released by treating an
insulin - sensitive glycophospholipid with a
phosphatidylinositol - specific
phospholipase C (
PI - PLC ) stimulated
pyruvate dehydrogenase (
PDH ) in both subcellular and whole cell assays . -
pubmed
The effect of the polar head group on
PDH in the subcellular assay was blocked by
sodium fluoride , suggesting that the polar head group activated the
PDH phosphatase . -
pubmed
The
PDH - stimulating activity eluted after
bacitracin but ahead of
ATP during gel filtration chromatography , and it was destroyed by exposure to
NH4OH or
alkaline phosphatase and by boiling in water . -
pubmed
Glucagon -( 1 - 21 )- peptide stimulated
[ 3H ] thymidine incorporation of mucosal cells significantly in a dose - dependent manner . -
pubmed
Then
glucagon - related peptides which have common sequences with
glucagon -( 1 - 21 )- peptide were also tested . -
pubmed
The
sulfonamides are separated from each other and from co - extracted materials on a C-18 reverse - phase column and detected at 450 nm following
post - column derivatization with
dimethylaminobenzaldehyde . -
pubmed
Sulfamerazine is a good internal standard for
sulfamethazine ; neither is ideal for
sulfathiazole.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
L364718 ( 20 nmol / kg ) caused a potent inhibition of
CCK-8 stimulated pancreatic protein ,
amylase and
trypsin secretion but not of volume and
bicarbonate secretion . -
pubmed
Release of
PP by
CCK was also significantly suppressed by
L364718 . -
pubmed
The possible mechanism of
phosphatidic acid - mediated cell stimulation and its relation to other
growth factors are discussed . -
pubmed
Based on
SDS PAGE and the action of
neuraminidase , the
acetylcholine receptor - inducing activity (
ARIA ) appears to be a 42,000 - D glycoprotein . -
pubmed
ARIA was extracted in a
trifluoroacetic acid - containing cocktail and purified to homogeneity by reverse - phase , ion exchange , and size exclusion high pressure liquid chromatography . -
pubmed
The 42 - kD
ARIA is extremely stable : it was not destroyed by exposure to intact myotubes , low pH , organic solvents , or
SDS . -
pubmed
The effect of
ARIA is apparently independent of the state of activity of the target myotubes as its effect on receptor incorporation added to that of maximal concentrations of
tetrodotoxin . -
pubmed
Esterases of the polymorphonuclear leukocyte capable of hydrolyzing
acetyl DL - phenyl - alanine beta - naphthyl ester . -
pubmed
Two of these
esterases ,
esterase 1 and
esterase 2 , are inhibited by various
p - nitrophenyl ethyl phosphonate esters . -
pubmed
The third
esterase ,
esterase 3 , differs from the other two by its inability to be inactivated by any of the
phosphonates no matter how high the concentration of
phosphonate or prolonged the period of incubation of cell with
phosphonate . -
pubmed
The inhibition profiles of
esterase 1 are distinctly different from those of
esterase 2 when the two
esterases are tested with the
phenylalkylphosphonates ,
chloroalkylphosphonates , and
alkylphosphonates . -
pubmed
The inhibition profile of
esterase 1 is essentially the same as that of the activatable
esterase of chemotaxis obtained previously when the same three homologous series of
phosphonates were tested for their ability to protect against deactivation by the chemotactic factor or give chemotactic - dependent inhibition . -
pubmed
After sedimentation of virus - containing sera through a
sucrose density gradient , colony inhibitory activity is present in the particulate fraction and separate from
serum immunoglobulins . -
pubmed
The components of the supernatant responsible for these activities have a molecular weight between 30,000 and 60,000 , and have the characteristics of
non - specific factors : they are genetically unrestricted , and do not bind to either
antigen or
anti - DR affinity columns . -
pubmed
While removing
iron and labile
sulphur from
ferredoxin reaction center the reduction wave of
Fe - S bonds with E 1/2 = -0.33 V ( N. H. E. ) transforms into the reduction wave of
S - S bonds with E 1/2 = -0,39 V at pH = 7 . -
pubmed
ZAP-70 binding specificity to
T cell receptor tyrosine - based activation motifs : the tandem
SH2 domains of
ZAP-70 bind distinct
tyrosine - based activation motifs with varying affinity . -
pubmed
Engagement of the
T cell antigen receptor (
TCR ) results in activation of several
tyrosine kinases leading to
tyrosine phosphorylation of
protein substrates and activation of multiple biochemical pathways . -
pubmed
TCR - mediated activation of the
src - family kinases ,
Lck and
Fyn , results in
tyrosine phosphorylation of the
TCR zeta and
CD3 chains . -
pubmed
The site of phosphorylation in these chains is the
tyrosine - based activation motif (
TAM ) , a 15 - 16
amino acid module containing two
tyrosine residues . -
pubmed
Tyrosine - phosphorylated TAMs serve as targets for binding of the
zeta - associated protein ( ZAP-70 ) tyrosine kinase via its tandem
SH2 domains . -
pubmed
Similar studies demonstrated that the
ZAP-70 tandem SH2 domain can bind a
TCR zeta 3 TAM peptide in which both
tyrosine residues are phosphorylated : Little or no binding was observed with peptides phosphorylated at only one
tyrosine residue , or a nonphosphorylated peptide . -
pubmed
All four doubly
tyrosine phosphorylated
TAM peptides cross - compete with each other for binding to the tandem
SH2 domains of
ZAP-70 . -
pubmed
The results provide further evidence that the
ZAP-70 interaction with the
TCR requires prior phosphorylation of both
tyrosine residues within a
TAM motif . -
pubmed
Binding of
ZAP-70 to
phospho - TAMs is notable for the high level of cooperativity between the two
SH2 domains , which individually demonstrate low affinity interaction with the ligand . -
pubmed
Affinity differences of as much as 30 - fold indicates a significant specificity of interaction of
ZAP-70 SH2 domains for different
phospho - TAMs . -
pubmed
In another example involving targeted
cytosine deaminase for the generation of
5 - fluorouracil , it is shown that as much as 17 times more drug can be delivered within a
tumor compared to when
5 - fluorouracil is administered alone . -
pubmed
The in vivo effect of
doxycycline treatment on
matrix metalloproteinases in
reactive arthritis . -
pubmed
The functional implications of these genomic footprinting data and the tissue - specific
CpG methylation patterns of the beta - like
globin promoters we obtained by genomic sequencing are discussed in terms of positive and negative regulation of the
human beta - like globin switch during erythroid development . -
pubmed
RESULTS : There were inverse relationships between survival time on one hand and plasma values of
noradrenaline ( r = -0.49 ; P & lt ; 0.01 ) and
atrial natriuretic peptide ( r = -0.49 ; P & lt ; 0.01 ) on the other hand . -
pubmed
There was a significant correlation between the plasma values of
noradrenaline and
atrial natriuretic peptide ( r = 0.50 ; P & lt ; 0.01 ) . -
pubmed
No significant differences were observed in the peak serum concentrations of
amylase between the groups treated with and treated without
ONO-5046 . -
pubmed
ONO-5046 significantly decreased the peak
serum lipase concentration . -
pubmed
However , pretreatment with
ONO-5046 significantly inhibited the rise in serum
CINC concentrations after reperfusion . -
pubmed
Pretreatment with
ONO-5046 also significantly inhibited the expression of
CINC mRNA transcripts in the graft . -
pubmed
However ,
ONO-5046 decreased
CINC production by peritoneal macrophages in response to
neutrophil elastase . -
pubmed
Preclinical profile of the mixed
5 - HT1A / 5 - HT2A receptor antagonist
S 21,357 . -
pubmed
This study evaluated the pharmacological and behavioral effects of
S 21,357 , a drug with high affinity for both
5 - HT1A and
5 - HT2A receptors . -
pubmed
The drug behaved as antagonist at both
5 - HT1A autoreceptors and postsynaptic
5 - HT1A receptors , as it prevented the inhibitory effect of
lesopitron on the electrical discharge of the dorsal raphe nucleus ( DRN ) 5 -
HT neurons and the activity of
forskolin - stimulated
adenylate cyclase in hippocampal homogenates . -
pubmed
When probed with
CsC1 gradients the relative
chloride permeability (
PC1 / PCs ) was estimated as 0.14 for
GluR - B(R ) and 0.74 for
GluR-6 ( R )- subunit channels . -
pubmed
Trans - FA have consistently been shown to elevate LDL -
cholesterol and
apolipoprotein B to a similar extent as
C12 - C16 saturated fatty acids . -
pubmed
[ 3H ] WIN 35,428 is a
cocaine analog that is useful for studying the distribution and density of the
dopamine transporter in striatum and other brain regions . -
pubmed
We have previously shown that fibroblasts from
ultra - violet ( UV ) hypersensitive xeroderma pigmentosum patients ( XP ) are markedly deficient in
catalase activity resulting in high intracellular levels of
hydrogen peroxide (
H2O2 ) following UV irradiation . -
pubmed
No direct correlation between
catalase activity and repair ability was found since XP variant cells which are proficient in
nucleotide excision repair (
NER ) showed activities as low as those found in
NER deficient classical XP groups A and D. However , in contrast to the
skin cancer prone
XP patients , another
NER deficient syndrome ,
trichothiodystrophy (
TTD ) , which does not exhibit any
cancer predisposition , was found to present normal
catalase activity . -
pubmed
These results incited us to examine the structure / function relationship of the tetrameric active enzyme form of
catalase ( which is the only one able to carry out
H2O2 dismutation ) with its cofactor
NADPH . -
pubmed
In the present study , we have measured the effects on
catalase activity after adding
NADPH either to acellular extracts or during cell culture of the different cell types . -
pubmed
Our results show a clear relationship between low
catalase activity and striking decrease in intracellular
NADPH levels . -
pubmed
Hopefully new agents such as
lazaroids ,
endothelins inhibitors and
nitric oxyde modulators will become available for clinical use in the next future . -
pubmed
BTK was activated in a dose- and time - dependent manner by treatment with the
tyrosine phosphatase inhibitor
pervanadate . -
pubmed
NAD +- dependent 15 - hydroxy - PG dehydrogenase (
PGDH ) is the major enzyme involved in the initial inactivation of PGs , and its activity is reduced by glucocorticoids ,
cortisol ( F ) , and
dexamethasone (
DEX ) . -
pubmed
In the placenta ,
11beta - HSD2 is the dominant isoform , acting as a
dehydrogenase [ F to
cortisone ( E ) ] ; and in chorion ,
11beta - HSD1 predominates as a
reductase ( E to F ) . -
pubmed
CBX alone had no effect on
PGDH activity in either placenta or chorion trophoblast cells . -
pubmed
In chorion , E significantly inhibited
PGDH activity , and this effect was reversed by addition of
CBX . -
pubmed
F and
DEX significantly inhibited
PGDH , and this effect was unaltered by coadministration of
CBX . -
pubmed
In contrast , in placenta , there was no effect of E , or of E with
CBX , on
PGDH activity . -
pubmed
However , F and
DEX inhibited
PGDH , and the effect of F ( but not
DEX ) was greater in the presence of
CBX . -
pubmed
When data from the
Neoral and
Sandimmune groups were combined , measurements of C0 but not
C2 or
C6 were associated with the risk of acute lung allograft rejection . -
pubmed
A chimeric form of
osteoprotegerin inhibits
hypercalcemia and
bone resorption induced by
IL-1beta ,
TNF - alpha ,
PTH ,
PTHrP , and 1 ,
25 ( OH ) 2D3 . -
pubmed
BDNF and its
tyrosine kinase receptor ,
TrkB , are expressed in hypothalamic nuclei associated with satiety and locomotor activity . -
pubmed
A 17 year old girl with active
Crohn ' s colitis developed
idiopathic thrombocytopenic purpura that was managed with intravenous
immune globulins and
cyclosporin A. -
pubmed
Phospholipase A2 , which is linked to a
protein kinase C pathway , hydrolyzes
phosphatidylcholine into
cis - unsaturated free fatty acids and
lysophosphatidylcholine (
lysoPC ) . -
pubmed
The results of the present study , thus , demonstrate that
phospholipase A2 - linked free
fatty acids and
lysoPC are employed in the sustained facilitation of hippocampal neurotransmission , suggesting a significant role of a
phospholipase A2 signaling pathway in the neuroplasticity . -
pubmed
Myeloperoxidase (
MPO ) has been involved in the pathogenesis of several diseases through excessive production of reactive
oxygen species ( ROS ) as well as through its genetic polymorphism . -
pubmed
The factors affecting
MPO levels were age , the number of white cells , smoking in fathers and
oral contraceptive intake in mothers . -
pubmed
The putative plasma membrane
Na (+)/ H (+ ) antiporter SOS1 controls long - distance
Na (+ ) transport in plants . -
pubmed
In this study , we examined the tissue - specific pattern of gene expression as well as the
Na (+ ) transport activity and subcellular localization of
SOS1 . -
pubmed
Under mild salt stress ( 25 mM
NaCl ) ,
sos1 mutant shoot accumulated less
Na(+ ) than did the wild - type shoot . -
pubmed
However , under severe salt stress ( 100 mM
NaCl ) ,
sos1 mutant plants accumulated more
Na(+ ) than did the wild type . -
pubmed
There also was greater
Na (+ ) content in the xylem sap of
sos1 mutant plants exposed to 100 mM
NaCl . -
pubmed
These results suggest that
SOS1 is critical for controlling long - distance
Na (+ ) transport from root to shoot . -
pubmed
We present a model in which
SOS1 functions in retrieving
Na (+ ) from the xylem stream under severe salt stress , whereas under mild salt stress it may function in loading
Na (+ ) into the xylem . -
pubmed
Statins and
low - density lipoprotein cholesterol levels . -
pubmed
In embryonic fibroblasts ,
epidermal growth factor and
12 - O - tetradecanoylphorbol-13 - acetate activated the
MAPK cascade to a similar extent , yet only
c - Raf-1 activation by
epidermal growth factor depended on
KSR1 . -
pubmed
RECOMBINATION OF
PROTOPORPHYRINOGEN WITH
CYTOCHROME C APOPROTEIN . -
pubmed
Analysis of conformational properties of the chimeric protein showed that all six conformational
mAbs interacted with the recombinant protein were reduction - sensitive , indicating that fusion of the two
cysteine - rich proteins retains critical conformational epitopes . -
pubmed
It was shown that the inhibition is dependent on the presence of
Abs to the chimeric protein and their
disulfide bond - dependent conformations . -
pubmed
Part
II : surface - enhanced Raman spectroscopy investigation of
methionine containing
heterodipeptides adsorbed on colloidal
silver . -
pubmed
Glutaric aciduria type I and
kynurenine pathway metabolites : a modified hypothesis . -
pubmed
Deficiency of glutaryl - CoA dehydrogenase blocking the
glutarate pathway and activation of
indoleamine 2,3 - dioxygenase in macrophages / monocytes by intercurrent
inflammation may increase flux down the
kynurenine pathway towards the production of
quinolinic acid . -
pubmed
Quinolinic acid is
neurotoxic and is an endogenous agonist at
N - methyl - D - aspartate receptors . -
pubmed
The two
Cr ( tfa ) 3 isomers extracted can be efficiently separated by a DB-210
GC column within 9 min . -
pubmed
This
depression was partially reversed by
8 - cyclopentlyl-1,3 - dimethylxanthine (
CPT ) , a selective
A1 adenosine receptor antagonist . -
pubmed
Accumulation of extracellular
adenosine and inhibition of
adenosine kinase activity were caused by hypercapnic
acidosis and isohydric
hypercapnia , but not isohydric
acidosis . -
pubmed
The inhibition of
adenosine kinase activity is suggested to cause the accumulation of extracellular
adenosine during
hypercapnia . -
pubmed
A laser - ablation molecular - beam Fourier transform microwave ( LA -
MB - FTMW ) spectrometer has been successfully applied to the structural study of
alpha - aminobutyric acid . -
pubmed
It was found that the former assimilated
formaldehyde via the
serine pathway whereas the latter did so via the
ribulose - monophosphate pathway from the measurement of the key
enzyme activities in each assimilation pathway . -
pubmed
We report the identification and characterization by thin - layer chromatography ( TLC ) of
N - acyl homoserine lactone ( HSL ) quorum sensing signal molecules by
PGP Burkholderia . -
pubmed
strain CBMB40 produced an additional molecule that migrated along
decanoyl ( C(10)- )
HSL . -
pubmed
The catalyzed signal amplification (
CSA ) technique , based on the peroxidase - mediated deposition of haptenized
tyramide and also known as
tyramide signal amplification and catalyzed reportor deposition systems , is widely accepted as a signal amplification method for immunohistochemistry and in situ hybridization . -
pubmed
In this study , we examined the applicability of a new simplified
CSA system employing
fluorescyl - tyramide ( FT ) to pathologic testing and research with
formalin - fixed ,
paraffin - embedded tissues . -
pubmed
By using the FT , instead of
biotinyl - tyramide ( BT ) that is commonly employed in the
CSA system with chromogen , nonspecific staining caused by endogenous
biotin was completely avoided . -
pubmed
Although
Kir4.1 channels are the major inwardly rectifying channels in glial cells and are widely accepted to support
K +- and
glutamate - uptake in the nervous system , the properties of
Kir4.1 channels during vital changes of
K + and
polyamines remain poorly understood . -
pubmed
The heterodimer of the
ecdysone receptor (
EcR ) and
ultraspiracle (
Usp ) , members of the
nuclear receptor superfamily , is considered to be functional receptor for the
ecdysteroids that coordinate essential biological processes in insects . -
pubmed
Ischemia /
reperfusion injury resulted in a decrease in the expression of
thioredoxin , while
tetramethylpyrazine administration greatly elevated the expression of
thioredoxin-1 /
thioredoxin-2 mRNA and
thioredoxin reductase-1 /
thioredoxin reductase-2 mRNA . -
pubmed
These findings suggest that administration of
tetramethylpyrazine , within a 4h time period
post - transient focal
stroke , may reduce
cerebral ischemic reperfusion damage . -
pubmed
Moreover , the neuroprotective effect of
tetramethylpyrazine may be mediated , in part , by an increase in genetic transcription of
thioredoxin . -
pubmed
RESULTS : Elementary modes analysis (
EMA ) of a set of
enzymes corresponding to the Krebs cycle , glycolysis and gluconeogenesis supports the scientific evidence showing that there is no pathway capable of converting
acetyl - CoA to
glucose at steady state . -
pubmed
This conversion is possible after the addition of
isocitrate lyase and
malate synthase ( forming the
glyoxylate shunt ) to the system . -
pubmed
Reducing the risk of
prostate cancer has gained increasing coverage in recent years , with proof of principle shown in the
Prostate Cancer Prevention Trial with the
type 2 5alpha - reductase (
5AR ) inhibitor ,
finasteride . -
pubmed
Several agents are under investigation for reducing the risk of
prostate cancer , including
selenium /
vitamin E and selective
oestrogen receptors modulators ( e.g. -
pubmed
In addition , the Reduction by
Dutasteride of
Prostate Cancer Events trial , involving & gt;8000 men , is evaluating the effect of the dual
5AR inhibitor ,
dutasteride , on the risk of developing
prostate cancer . -
pubmed
PURPOSE :
Vandetanib is a once - daily oral inhibitor of
vascular endothelial growth factor receptor (
VEGFR ) and
epidermal growth factor receptor (
EGFR ) signaling . -
pubmed
In this two - part phase II study , the efficacy and safety of
vandetanib was compared with that of
gefitinib , an inhibitor of
EGFR signaling . -
pubmed
AIM :
Heparanase , a
glycohydrolase enzyme , cleaves
heparan sulfate in the tissue matrix . -
pubmed
On further investigation he had grade
II hepatocellular
iron staining and a hepatic index of 5.4 leading to a diagnosis of
hereditary hemochromatosis . -
pubmed
Exogenous soluble
VEGF receptor-1 (
sFlt-1 ) regulates Th1 / Th2
cytokine production from normal placental explants via intracellular
calcium . -
pubmed
The aim of this study is to examine if exogenous
sFlt-1 can regulate Th1 / Th2
cytokines IL-10 and
TNF - alpha production from normal placental explants via intracellular
calcium release . -
pubmed
Intracellular
calcium blocker ,
1,2 - bis -( o - aminophenoxy )- ethane - N , N , N ' , N '- tetraacetic acid ,
tetra ( acetoxymethyl )- ester (
BAPTA / AM ) was applied to investigate whether the changes in
cytokine concentration were mediated by intracellular free
calcium . -
pubmed
The increase in
IL-10 can be inhibited by
BAPTA /
AM . -
pubmed
The change in Th1 / Th2
cytokine level may be mediated by intracellular free
calcium . -
pubmed
Moreover , the co - purified catalytic domain plus site - directed mutants of the
D123 protein lacking these aromatic residues showed that W366 was key to the apparent affinity for the polysaccharide substrate of
starch synthase III , whereas either of these
amino acid residues altered
ADP -
glucose kinetics . -
pubmed
The sensitivity of the direct wet smear was 45.8 % , that of the prolonged May - Grunwald Giemsa staining was 58.3 % , and that of the
SAF /
methylene blue method was 62.5 % . -
pubmed
Both
ATP and
adenosine suppress cardiac pacemakers ' automaticity and atrioventricular
nodal conduction , albeit via the different mechanism of actions . -
pubmed
Canonical
Wnt3a modulates intracellular
calcium and enhances excitatory neurotransmission in hippocampal neurons . -
pubmed
Our results identify the
Wnt3a protein and a member of its complex receptor at the membrane , the
low density lipoprotein receptor - related protein 6 (
LRP6 ) coreceptor , as key molecules in neurotransmission modulation and suggest cross - talk between canonical and Wnt /
Ca ( 2 + ) signaling in central neurons . -
pubmed
Three sterically crowded
peri - substituted naphthalene phosphines ,
Nap [ PPh ( 2 )][ ER ] (
Nap =
naphthalene-1,8 - diyl ;
ER =
SEt ,
SPh ,
SePh ) 1 - 3 , which contain
phosphorus and
chalcogen functional groups at the peri positions have been prepared . -
pubmed
M and further pharmacological experiments suggested that the
receptors involved in this inhibitory effect are of the
H ( 3 ) type . -
pubmed
Also in view of data reported by others it is suggested tentatively that in general presynaptic modulation of neurotransmitter release by
histamine , if it occurs , involves activation of
H ( 3 ) receptors . -
pubmed
Immunoblotting and immunohistochemistry were used to determine the
phospho -
Akt ( pAkt
Ser473 ) ,
phospho -
eNOS ( peNOS Ser1177 ) , and
eNOS levels and immunofluorescence to determine the cellular distribution of
eNOS and pAkt
Ser473 . -
pubmed
Wortmannin treatment before LPS preconditioning significantly reduced
eNOS expression in neurons and endothelial cells , which inhibited LPS - induced protection against
HI . -
pubmed
Phosphatidylinositol 5 - phosphate links
dehydration stress to the activity of
ARABIDOPSIS TRITHORAX - LIKE factor ATX1 . -
pubmed
Interestingly ,
ATX1 contains a
PtdIns5P interaction domain ( PHD finger ) and thus ,
phosphoinositide signaling , may link environmental stress to changes in gene transcription . -
pubmed
HVOD occurred during chemotherapy protocols of CAM (
CTX +
Ara - C + 6 - TG ) or maintenance period (
MTX + 6 -
TG ) . -
pubmed
Predicting the outcome of grade
II glioma treated with
temozolomide using proton magnetic resonance spectroscopy . -
pubmed
BACKGROUND : This study was designed to evaluate proton magnetic resonance spectroscopy (
( 1 ) H -
MRS ) for monitoring the WHO grade II
glioma (
low - grade glioma ( LGG ) ) treated with
temozolomide (
TMZ ) . -
pubmed
Response to
TMZ was evaluated by monthly MRI examinations that included MRI with volumetric calculations and
( 1 ) H -
MRS for assessing Cho /
Cr and
Cho /
NAA ratios . -
pubmed
Thus ,
( 1 ) H -
MRS may be a promising , non - invasive tool for predicting and monitoring the clinical response to
TMZ . -
pubmed
Among the 10
DNA polymerase mutations selected with
ganciclovir , 7 ( P488R , C539R , L545S , V787L , V812L , P829S , and L862F ) were associated with
ganciclovir resistance , whereas 2 ( F595I and V946L ) conferred only
foscarnet resistance . -
pubmed
Moreover , compared with
KCl and NE ,
ANG II contractions were diminished & gt;= 5 - fold . -
pubmed
Whereas
KCl and
ANG II contracted third generation>>precaruncular / placental UVSM , NE - induced contractions were similar throughout the UVB . -
pubmed
Expanded
polytetrafluoroethylene (
ePTFE ) is unique in that it can be visualized on computed tomography (
CT ) . -
pubmed
Our findings suggest that
CR activates mitochondrial energy metabolism and
fatty acid biosynthesis in WAT . -
pubmed
In contrast , in BAT
CR either had no effect on , or down - regulated , the mitochondrial electron transport chain , but enhanced
fatty acid biosynthesis . -
pubmed
O - GlcNAcylation is an unusual form of protein glycosylation , where a single -
sugar [
GlcNAc (
N - acetylglucosamine ) ] is added ( via beta - attachment ) to the
hydroxyl moiety of
serine and
threonine residues of nuclear and
cytoplasmic proteins . -
pubmed
We have previously described a novel
mGlu5 positive allosteric modulator (
PAM ) ,
LSN2463359 and provided evidence of its ability to attenuate aspects of the behavioural response to administration of the competitive
NMDA receptor antagonist ,
SDZ 220,581 . -
pubmed
In the present study , the interactions between this
mGlu5 PAM and the
NMDA receptor were explored further by assessing the effects of
LSN2463359 against some of the motor , instrumental and cognitive effects induced by the non - competitive
NMDA receptor antagonists
PCP and
MK-801 , the competitive
NMDA receptor antagonist
SDZ 220,581 and the
GluN2B selective
NMDA receptor antagonist ,
Ro 63 - 1908 . -
pubmed
Of several chemical chaperones tested , the addition of
betaine resulted in increased secretion of
FVIII , by increasing solubility of intracellular
FVIII aggregates and improving transport from endoplasmic reticulum to Golgi . -
pubmed
Moreover , in vitro and in vivo applications of
betaine were also able to rescue a trafficking - defective
FVIII mutant (
FVIIIQ305P ) . -
pubmed
In addition ,
megakaryocyte - specific cell surface markers showed a dramatic increase in expression in response to
( R )- TEMOSPho treatment . -
pubmed
We detected
caspase - dependent cleavage substrates by quantifying protein intensities before and after
TRAIL induction in
SDS gel slices . -
pubmed
RESULTS : the
ICER of the adding - on regimens range from negative values for
Gabapentin ,
Levetiracetam and
Zonisamide to low values for
Lamotrigine ( ~ 6.4 million Rials / consequence [ mil Rls / consq ] ) and
Oxcarbazepine ( ~7.7 mil Rls / consq ) and followed by high values for
Topiramate ( ~21 mil Rls / consq ) and
Vigabatrin ( ~43.7 mil Rls / consq ) considering the three months of remaining on regimen . -
pubmed
The results showed that KP - SMEDDS and
KP-2 - HP - beta - CD complex improved the dissolution rate of
methoxyflavones in both 0.1N
HCl and 0.2 M
PBS pH 6.8 compared to KP dissolved in a solution of
propylene glycol ,
PEG 400 ,
ethanol , and water . -
pubmed
The oral bioavailability values of KP - SMEDDS formulations were higher than those of KP ( 25.38- , 42.00- , and 26.01 - fold for
PMF ,
TMF , and
DMF , respectively ) . -
pubmed
Zinc as mediator of
ubiquitin conjugation following
traumatic brain injury . -
pubmed
Here we assessed the effect of
zinc on
ubiquitin conjugation and subsequent neurodegeration following
traumatic brain injury (
TBI ) . -
pubmed
Our previous study has shown that
zinc can induce
ubiquitin conjugation in cultured hippocampal neurons . -
pubmed
RESULTS : In the ISOR sample ,
APOE epsilon4 allele carriers had higher plasma
triglyceride ( p=0.0002 ) , total
cholesterol ( p=0.009 ) and
LDL -
cholesterol ( p=0.003 ) levels than epsilon3 allele carriers . -
pubmed
Linkage disequilibrium and haplotype analyses confirmed the respectively deleterious and protective impacts of the epsilon4 and epsilon2 alleles on
LDL -
cholesterol levels and showed that the G allele of the rs4420638 polymorphism may exert a protective effect on
LDL -
cholesterol levels in subjects bearing the
APOE epsilon 4 allele . -
pubmed
CONCLUSION : Our results showed that ( i ) the
APOE epsilon polymorphism has the expected impact on the plasma lipid profile and ( ii ) the rs4420638 G allele may counterbalance the deleterious effect of the epsilon4 allele on
LDL -
cholesterol levels in an Algerian population . -
pubmed
The
potassium chloride cotransporters ( KCC ) family of proteins are widely expressed and are involved in the transepithelial movement of
potassium and
chloride ions and the regulation of cell volume . -
pubmed
RESULTS : While the maximum specific growth rates of the mutants and the parental
strain were similar at both temperatures ( 0.33 + - 0.02 h(-1 ) and 0.34 + - 0.03 h(-1 ) , respectively ) , DNA microarray analyses revealed that the
CtsR or
HrcA deficient
strains displayed altered transcription patterns of genes encoding functions involved in transport and binding of
sugars and other compounds , primary metabolism , transcription regulation , capsular polysaccharide biosynthesis , as well as
fatty acid metabolism . -
pubmed
The
ANP group ( n = 40 ) was injected with 4.5 %
sodium taurocholate into the pancreatic duct to induce
ANP . -
pubmed
RESULTS :
IAP showed a continuous upward trend in the
ANP group , with values 2 to 3 times higher than those in the SO group at the corresponding time points and the rising rate was peaking at 6 h. The levels of
plasma amylase ,
TNF - alpha ,
Cr , TB ,
DAO , and
D - lactate also gradually increased in the
ANP group over time and were significantly higher than in the SO group at 3 , 6 , 12 and 24 h ( all P<0.05 ) . -
pubmed
The protocol also allows the one -
pot transformation of
aromatic nitro compounds into
dimethylated amines in the presence of a simple
iron catalyst . -
pubmed
We have demonstrated that deleting a dispensable gene involved in
galactose metabolism (
ugeA ) and one involved in biosynthesis of a minor cell wall component ( ugmA ) led to changes in hyphal elemental distribution that may have resulted from compromised wall composition . -
pubmed
RESULTS : In the probability of target attainment (
PTA ) analyses , only 2 g of iv
vancomycin every 24 h conferred & gt;90 % probability of achieving an AUC / MIC ratio of & gt;400 for MIC values & lt;2 mg / L in the serum . -
pubmed
CVD risk factors included
diabetes ( self - reported diagnosis ,
glycated hemoglobin & gt;=6.5 % , or fasting plasma
glucose & gt;=126mg / dl ) ,
hypertension ( treatment or blood pressure & gt;=140/90 mmHg ) ,
dyslipidemia ( treatment or non -
HDL cholesterol & gt;=160 mg / dl ) , and smoking ( self - report or
cotinine levels & gt;=10 ng / ml ) . -
pubmed
The short - term incorporation of
13C (
H13CO3 ) in phytoplankton and bacterial lipid biomarkers was measured as well as the total bacterial
carbon production ( BP ) , viral lysis and the microbial community structure under three experimental conditions : ( 1 ) High inorganic Nutrient and High Grazing ( HN + HG ) , ( 2 ) High inorganic Nutrient and Low Grazing ( HN + LG ) and ( 3 ) under natural in situ conditions with Low inorganic Nutrient and High Grazing ( LN + HG ) during
spring . -
pubmed
Theanine improves the function of dendritic cells via the downregulation of
cyclooxygenase-2 expression . -
pubmed
We sought to measure
cyclooxygenase-2 (
COX-2 ) expression in bombesin - inhibited DCs treated with
theanine in vitro and to explore the protection and activation effects of
theanine on DCs . -
pubmed
METHODS : Enzyme - linked immunosorbent assay ( ELISA ) , reverse transcription -
polymerase chain reaction ( RT - PCR ) , and Western blotting were used to analyze the effects of
theanine on
COX-2 expression and
interleukin ( IL )- 12 /
IL-10 secretion of
bombesin - treated DCs . -
pubmed
Theanine increased the expression of
mature DC surface molecules . -
pubmed
COX-2 expression in
bombesin - treated DCs was inhibited by
theanine in a dose - dependent manner . -
pubmed
Theanine promoted DC secretion of
IL-12 . -
pubmed
IL-12 levels reached ( 137.4 + - 4.9 ) pg / ml with
theanine at 200 micromol / L. However ,
theanine inhibited the secretion of
IL-10 in a dose - dependent manner . -
pubmed
IL-10 levels were only ( 58.4 + - 6.9 ) pg / ml with
theanine at 200 micromol / L. CONCLUSION :
Theanine inhibits the transcription and translation of
COX-2 and regulates the balance of
IL-10 /
IL-12 secretion in
bombesin - inhibited DCs , leading to the recovery of a state of activation in DCs . -
pubmed
Dynamic and coordinated regulation of
KEAP1 -
NRF2 - ARE and
p53 /
p21 signaling pathways is associated with
acetaminophen injury responsive liver regeneration . -
pubmed
The expression and nuclear accumulation of
NRF2 was increased after
APAP treatment . -
pubmed
The content of
p53 and its downstream target
p21 were significantly increased upon
APAP exposure and subsequently decreased to normal levels at 48 hours . -
pubmed
These results demonstrated that dynamic and coordinated regulation of
KEAP1 -
NRF2 - ARE and
p53 /
p21 signaling pathways was associated with compensatory liver regeneration after
APAP - induced
acute liver injury . -
pubmed
RECENT FINDINGS : Since the landmark discovery that
bone morphogenetic protein receptor type II (
BMPR2 ) mutations cause the majority of cases of
familial PAH , investigators have discovered mutations in genes that cause
PAH in families without
BMPR2 mutations , including the type I receptor
ACVRL1 and the type III receptor
ENG ( both associated with
hereditary hemorrhagic telangiectasia ) ,
caveolin-1 (
CAV1 ) , and a gene (
KCNK3 ) encoding a two - pore
potassium channel . -
pubmed
We describe the use of LC -
MS to analyse DNA and RNA
oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light - directed in situ chemistry . -
pubmed
The aim of this work was to evaluate the influence of the adsorption process with granular activated
carbon (
GAC ) on the
chlorine dioxide consumption in final oxidation / disinfection . -
pubmed
Moreover , a good correlation was found between the
ClO2 - D and UV absorbance at wavelength 254 nm using mineral
carbons ; therefore , the use of a mineral mesoporous
GAC is an effective solution to control the high
ClO2 - D in the disinfection stage of a DWTP . -
pubmed
Cocrystal structures of central region of
PfAMA1 containing
disulfide - linked clusters ( domains I and II ) with
R1 peptide and a peptide derived from
PfRON2 showed strong structural similarity in binding . -
pubmed
The cross - linking capacity of homodimeric
lectins was enhanced by covalent connection of the two
carbohydrate - binding sites . -
pubmed
The bone mineral density (
BMD ) of the lumbar vertebra and hip joint , and the level of
serum bone gla protein (
S - BGP ) and
hydroxyproline /
creatinine (
Hyp /
Cr ) were observed before and after treatment in the two groups . -
pubmed
( 2)After treatment , the index of bone metabolism in the control group was increased , and the serum S -
BGP , the
Hyp /
Cr in the control group were higher than those in the observation group ( both P<0 . -
pubmed
plantarum
maltose - producing
alpha - amylase (
LpMA ) belongs to
glycoside hydrolase family 13 . -
pubmed
It produced only G2 from the hydrolysis of various substrates , including
maltotriose (
G3 ) ,
maltopentaose (
G5 ) ,
maltosyl beta - cyclodextrin (
G2 - beta - CD ) , amylose , amylopectin , and starch . -
pubmed
Reaction pattern analysis using
4 - nitrophenyl - alpha - d - maltopentaoside (
pNPG5 ) demonstrated that
LpMA hydrolyzed
pNPG5 from the nonreducing end , indicating that LpMA is an
exotype alpha - amylase . -
pubmed
Kinetic analysis revealed that
LpMA had the highest catalytic efficiency ( kcat / Km ratio ) toward
G2 - beta - CD . -
pubmed
BAEO was isolated by hydrodistillation , and gas chromatography - mass spectrometry (
GC -
MS ) analysis identified
benzaldehyde ( 75.35 % ) ,
benzoic acid ( 6.21 % ) and
mandelonitrile ( 5.38 % ) . -
pubmed
Z - VAD - FMK , a broad - spectrum
caspase inhibitor , attenuated
BAEO - induced apoptosis . -
pubmed
Also , increased
Bax and decreased
Bcl-2 levels suggest that
BAEO - induced apoptosis is mediated through both
death receptor and mitochondrial pathways . -
pubmed
Moreover , reduced
Rel / NF - kappaB levels suggest that
BAEO - mediated apoptosis is also associated with inhibition of the
NF - kappaB pathway . -
pubmed
NAC was diluted in
MHB to a starting concentration of 160 mg / mL and serial two - fold microdilution assays were performed in triplicate with negative controls for all isolates tested . -
pubmed
RESULTS : There were significant reduction in the levels of the
interleukin-6 and
cardiac troponin - I in the
trimetazidine - treated group ( group 2 ) compared to the control group ( group 1 ) ( P<0.001 ) , after 12h and 24h
post - operative . -
pubmed
beta - Catenin signaling positively regulates
glutamate uptake and metabolism in astrocytes . -
pubmed
CONCLUSIONS : These studies confirm that
beta - catenin regulates key proteins responsible for excitatory
glutamate neurotransmission in vitro and in vivo and reveal the therapeutic potential of
beta - catenin modulation in treating diseases with abnormal glutamatergic neurotransmission and excitotoxicity . -
pubmed
Purpose : The purpose of this study was to evaluate the antitumor efficacy of the reduced immunogenicity anti -
mesothelin immunotoxin RG7787 plus nab -
paclitaxel against primary
mesothelioma cell lines and tumor xenografts and the utility of
mesothelin as a biomarker of
tumor response . -
pubmed
Conclusions : RG7787 plus nab -
paclitaxel is very active against primary
human mesothelioma cells in vitro as well as in vivo , with serum
mesothelin levels correlating with
tumor response . -
pubmed
To induce neuroprogenitor cells , EBs were cultured in the presence of
retinoic acid , N2 supplement and
fibroblast growth factor followed by culturing in a neurobasal medium containing
B27 ,
N2 supplements for additional 10 days , to allow the maturation and development of neuronal progenitor cells . -
pubmed
In particular , the
tumor microenvironment includes elevated levels of
ATP required for activation of
P2X7R and the sustained
tumor and immune cell
P2X7R - mediated responses which in total contribute to overall
tumor growth and viability . -
pubmed
Supplementation of
RLP significantly decreased semen
Malondialdehyde (
MDA ) concentration . -
pubmed
Androgen Triggers the Pro - Migratory
CXCL12 /
CXCR4 Axis in
AR - Positive
Breast Cancer Cell Lines : Underlying Mechanism and Possible Implications for the Use of
Aromatase Inhibitors in
Breast Cancer . -
pubmed
We have shown that
androgen induces
C - X - C motif chemokine 12 (
CXCL12 ) in
BC cell lines . -
pubmed
Our aim was to identify the mechanisms regulating the phenotypic effects of
androgen - induced
CXCL12 on
Androgen Receptor (
AR ) positive
BC cell lines . -
pubmed
The
steroid receptor co - regulator
NCOA1 is critical for this effect . -
pubmed
A synbiotic - containing
amino - acid - based formula improves gut microbiota in non -
IgE - mediated
allergic infants . -
pubmed
Annexin V /
PI and cell cycle analysis showed the maximum apoptosis - inducing effect of the
dichloromethane fraction at 200 mug / mL. -
pubmed
The Effect of the Oral Administration of
Leucine on Endothelial Function ,
Glucose and
Insulin Concentrations in Healthy Subjects . -
pubmed
Serum
glucose and
insulin were measured at baseline and every 10 min after
glucose infusion for 1 h. Endothelial function was evaluated by measurement of flow mediated vasodilatation (
FMD ) at baseline , 10 and 60 min after
glucose infusion . -
pubmed
RESULTS : In both visits ,
glucose levels increased to the same degree , whereas
insulin response was significantly higher after
leucine administration . -
pubmed
Leucine administration prevents
hyperglycaemia - mediated
endothelial dysfunction probably due to enhanced
insulin secretion . -
pubmed
Previous studies have found that
renin -
angiotensin -
aldosterone system ( RAAS ) played vital and bidirectional roles in
ALI . -
pubmed
The expression levels of
angiotensinII (
AngII ) and
angiotensin -( 1 - 7 ) (
Ang -( 1 - 7 ) ) were measured through ELISA . -
pubmed
There were notable seasonal changes in
PAH concentrations : winter & gt ; autumn & gt ;
spring & gt ; summer . -
pubmed
CONCLUSIONS : For teeth restored with adhesive procedures and
lithium disilicate onlays , the presence of build - up with fiber
post to provide retention and resistance form does not influence the
fracture strength . -
pubmed
In the present study , using
GFP -
ATG8 gene as a marker for tracking putative autophagosomes , we confirmed that autophagic vacuolization may lead to autophagic cell
death in the
DBA - treated parasites . -
pubmed
Saliva samples were plated in
Mannitol salt , and isolates were identified by
DnaJ gene sequencing . -
pubmed
But we blaze a trail by amalgamating the pharmacological mechanistic studies of
KYNA in kinship with
alpha7nAChRs , NMDARs and
GABA which lends aid in favour of KA . -
pubmed
We report a crystal structure of this divergent
Cas6 , identify
amino acids required for
Cas6 activity , show that the Cas6 domain is required for
RT activity and RNA spacer acquisition , and demonstrate that CRISPR - repeat binding to
Cas6 regulates
RT activity . -
pubmed
But none of the treatments either in in vitro or ex vivo cardiac models , induced a modulation of
desmin , phosphorylated and O - GlcNAcylated
desmin expression , despite the presence of
O - GlcNAc moities in cardiac
desmin . -
pubmed
Here we design and synthesize two D - A polymers which both carry a fluorinated
quinoxaline acceptor unit , but while
P1 includes a
benzodithiophene donor moiety with
thiophene side - groups ( 2D - BDT ) , the
P2 polymer lacks 2D conjugation in its simpler
pentathiophene donor segment . -
pubmed
However , poor fill factors limit the preliminary power conversion efficiencies to 3.3 % for
P1 and 1.0 % for
P2 as blended with
PCBM[70 ] in a 1 : 1.5 ( w / w ) ratio . -
pubmed
UH3 - 1 , a newly isolated anaerobic gut fungus , grows on diverse untreated substrates through production of a broad range of
carbohydrate active
enzymes that are robust to variations in substrate composition . -
pubmed
Neither
tacrolimus ( OR , 0.75 ; 95 % CI , 0.47 - 1.21 ; p = 0.24 ) nor
cyclosporine ( OR , 1.40 ; 95 % CI , 0.89 - 2.18 ; p = 0.14 ) were related significantly with
post - LTx
obesity . -
pubmed
One of the most common mechanisms attributed to spectral shifts in
FP is excited state proton transfer ( ESPT ) ,
hydroxyl moiety protonation and deprotonation , along with chromophore cis - trans isomerism . -
pubmed
Tamoxifen 's interference potential may be countered by using higher iCR doses or iCR +
HP . -
pubmed
The present study demonstrated that
cyclic adenosine monophosphate signaling in a wounded cell was induced by autocrine purinergic signaling , and
protein kinase A potentiates membrane resealing for repeated
wounds in those cells . -
pubmed
A new coordination polymer (
CP ) , namely
poly [( mu-4,4 '- bipyridine )( mu3 - 3,4 '- oxydibenzoato ) cobalt ( II ) ] ,
[ Co ( C14H8O5 )( C10H8N2 )] n or
[ Co ( 3,4 '- obb )( 4,4 '- bipy )] n ( 1 ) , was prepared by the self - assembly of
Co ( NO3 ) 2 6H2O with the rarely used
3,4 '- oxydibenzoic acid ( 3,4 '- obbH2 ) ligand and
4,4 '- bipyridine ( 4,4 '- bipy ) under solvothermal conditions , and has been structurally characterized by elemental analysis , IR spectroscopy , single - crystal X - ray crystallography and powder X - ray diffraction ( PXRD ) . -
pubmed
Circulating levels of
glucose ,
prolactin ,
testosterone ,
gonadotropins , thyrotropin , and free thyroid hormones were measured , while the structure parameters of thyroid homeostasis and the degree of
insulin sensitivity were calculated at baseline and 16 weeks later . -
pubmed
In both study groups ,
metformin decreased plasma
glucose levels and improved
insulin sensitivity . -
pubmed
Metformin - induced changes in
thyrotropin and Jostel 's index correlated with their baseline values , baseline levels of
testosterone , and with the effect of treatment on
insulin sensitivity . -
pubmed
Redox regulation of hepatic
NLRP3 inflammasome activation and immune dysregulation in
trichloroethene - mediated
autoimmunity . -
pubmed
In addition ,
TCE exposure led to prominent lobular
inflammation with sinusoid dilation , increased sinusoidal cellularity and increased staining for
proliferating cell nuclear antigen (
PCNA ) , confirming inflammatory and hepatocellular cell proliferation . -
pubmed
Importantly ,
TCE exposure resulted in the activation of hepatic inflammasome (
NLRP3 and
caspase-1 ) and up - regulation of pro - inflammatory cytokine
IL-1beta , and these changes were attenuated by
NAC supplementation . -
pubmed
Antileukemic Efficacy in Vitro of
Talazoparib and
APE1 Inhibitor III Combined with
Decitabine in
Myeloid Malignancies . -
pubmed
Here , we investigated the cytotoxic efficacy of
talazoparib and
APE1 inhibitor III , inhibitors of
PARP1 / 2 and
APE1 , in primary
CD34 +
MDS /
CMML cell samples ( n = 8 ; 4
MDS and 4
CMML ) and in primary
CD34 + or
CD34- AML cell samples ( n = 18 ) in comparison to healthy
CD34 + donor cell samples ( n = 8) . -
pubmed
Strikingly ,
talazoparib and
APE1 inhibitor III demonstrated critical antileukemic efficacy in selected
MDS /
CMML and
AML cell samples . -
pubmed
Low doses of
talazoparib and
APE1 inhibitor III further increased the cytotoxic efficacy of
decitabine in
MDS /
CMML and
AML cells . -
pubmed
Moreover , low doses of
APE1 inhibitor III increased the cytotoxic efficacy of
talazoparib in
MDS /
CMML and
AML cells . -
pubmed
In summary ,
talazoparib and
APE1 inhibitor III demonstrated substantial antileukemic efficacy as single agents , in combination with
decitabine , and combined with each other . -
pubmed
Nonetheless ,
T2 * -based methods become challenging when fat and
iron are simultaneously present . -
pubmed
This study proposes a phantom design concomitantly containing various concentrations of
iron and fat suitable for devising accurate simultaneous
T2 * and fat quantification technique . -
pubmed
MATERIALS AND METHODS : A 46 - vial
iron - fat - water phantom with various
iron concentrations covering clinically relevant
T2 * relaxation time values , from healthy to severely overloaded liver and wide fat percentages ranges from 0 to 100 % was prepared . -
pubmed
T2 * relaxation times and fat fractions were extracted from the MR signals to explore the effects of fat and
iron overload . -
pubmed
Therefore ,
T2 * values from the mono - exponential signal equation were used and expressed the relationship between relaxation time value across all
iron (
Fe ) and fat concentration as [ Formula : see text ] , with R - squared = 0.89 . -
pubmed
DISCUSSION : The proposed phantom design with microsphere
iron particles closely simulated the single -
T2 * behavior of
fatty iron - overloaded liver in vivo . -
pubmed
Cetuximab enhances the efficiency of
irinotecan through simultaneously inhibiting the
MAPK signaling and
ABCG2 in
colorectal cancer cells . -
pubmed
RESULTS : Cetuximab was found to increase intracellular concentrations of
irinotecan as well as
cytotoxicity by inhibiting the
epidermal growth factor receptor and , by extension , the downstream
RAS -
RAF -
MEK -
ERK signaling pathway . -
pubmed
It was also shown that
cetuximab inhibited the drug efflux activity of
ABCG2 . -
pubmed
In combination with
irinotecan ,
cetuximab can both significantly induce cell apoptosis by inhibiting the
RAS -
RAF -
MEK -
ERK signaling pathway and improve the effects of
irinotecan by decreasing drug efflux through the inhibition of
ABCG2 . -
pubmed
Terrestrial Fluxes of
Carbon in
GCP Carbon Budgets . -
pubmed
The Global
Carbon Project (
GCP ) has published global
carbon budgets annually since 2007 ( Raupach et al . -
pubmed
The
GCP budget is composed of annual perturbations from pre - industrial conditions , driven by additions of
carbon to the system from combustion of fossil fuels and by transfers of
carbon from land to the atmosphere as a result of land use . -
pubmed
Although the
GCP budgets have focused on the two net terrestrial fluxes , they have paid little attention to the gross components , which are important for a number of reasons , including understanding the potential for land management to remove
CO2 from the atmosphere and understanding the processes responsible for the sink for
carbon on land . -
pubmed
In this study , analytical techniques were combined with enzymatic assays to develop a novel method for the detection of
snake venom protease activity by using
rhodamine-110 - peptide substrate . -
pubmed
The application of the
rhodamine-110 - peptide substrate assay showed significantly greater sensitivity compared to prior assays using
casein -
FITC as the substrate . -
pubmed
Nicotine - induced degradation of
elastin and
collagen fibers were significantly suppressed in BN group . -
pubmed
These results suggest that
proanthocyanidins - rich BSSCE can prevent the weakening of the aortic wall via inhibiting
MMP-2 upregulation . -
pubmed
Two
tyrosine kinase inhibitors ,
vandetanib and
cabozantinib , have been approved for treatment of advanced
MTC , but options beyond those are limited . -
pubmed
Sulfanylmethyldimethylaminopyridine as a Useful
Thiol Additive for Ligation Chemistry in Peptide /
Protein Synthesis . -
pubmed
The
alkyl thiol in 2 allows it to be used for the one -
pot native chemical ligation - desulfurization protocol in peptide synthesis . -
pubmed
The
levodopa and Bushen Zhichan recipe group exhibited decreased
glutamate receptors and increased
gamma - aminobutyric acid receptors in the striatum . -
pubmed
This translocation of
TrkB depends on co - factors and modulators of
cAMP levels , N - glycosylation , and receptor transactivation . -
pubmed
Personalized
warfarin treatment based on the
PITX2 single
nucleotide polymorphism rs6843082 . -
pubmed
In therapies targeting
EGFR , the development of resistance owing to mutations and single
nucleotide polymorphisms , and the expression of the receptor ligands themselves are very serious issues . -
pubmed
However ,
neonatal jaundice (
hyperbilirubinemia ) derived from
HO overexpression led to the development of inhibitors , such as those based on
metaloproto- and
meso - porphyrins inhibitors with competitive activity . -
pubmed
In vitro , 10 microM
norepinephrine markedly downregulated the expressions of
prolactin ,
IGFBP1 , and
PLZF , which are the specifical markers of decidual stromal cells during decidualization . -
pubmed
The
alpha1b - adrenergic receptor expression was upregulated by
norepinephrine . -
pubmed
Interestingly ,
norepinephrine did not inhibit the expression of
IGFBP1 in endometrial stromal cells after silencing
alpha1b - adrenergic receptor , while significantly suppressed the induced decidualization with overexpression of
alpha1b - adrenergic receptor . -
pubmed
When
alpha1b - adrenergic receptor was activated , endometrial p -
PKC was significantly increased under
post - treatment with
norepinephrine in vivo and in vitro . -
pubmed
Therefore ,
norepinephrine exposure inhibited endometrial decidualization through the activation of the
PKC signaling pathway by upregulating
alpha1b - adrenergic receptor . -
pubmed
While few findings were replicated by multiple strong studies ,
brain derived neurotrophic factor (
BDNF ) and
gamma aminobutyric acid (
GABA ) in
depression ,
BDNF in
post -
stroke deficits , and
beta - endorphin in
chronic pain may be altered by rTMS . -
pubmed
CONCLUSION :
BDNF ,
GABA and
beta - endorphin were identified as potential molecular markers of rTMS and warrant further exploration . -
pubmed
RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of
cholesterol moved from RBCs to
HDL , while
cholesterol from
LDL moved in the opposite direction . -
pubmed
However , specific
cholesterol efflux from RBCs to isolated
apoA - I was negligible , and
ABCA1 silencing with siRNA or inhibition with
vanadate and
Probucol did not inhibit the efflux to
apoA - I ,
HDL , or plasma . -
pubmed
Bioinformatics analysis identified
ABCA7 ,
ABCG5 ,
lipoprotein lipase , and mitochondrial
translocator protein as possible candidates that may mediate the
cholesterol flux . -
pubmed
Pre - incubation of sections in media containing
formaldehyde and
glycine or with buffers of different pH , followed by incubation for
myosin - ATPase , also differentiated the muscle fibres into a specific distribution pattern within the myotomes , resembling that of the oxidative enzymes to some extent . -
pubmed
After 2 - -3 additional days in vitro these aggregates were shown by three different criteria to have developed such a conductance mechanism : ( a ) action potential upstroke velocities greater than 90 V / sec ; ( b ) suppression of spontaneous activity by
tetrodotoxin ; and ( c ) presence of a fast component of inward current recorded during voltage
clamp . -
pubmed
These include
hydrolases and nonenzymatic substances ordinarily contained within lysosomes , as well as
oxygen - derived free radicals . -
pubmed
The method depends on the enzymatic decolourization of the blue dye
dichlorophenolindophenol by
lactate in the presence of the enzyme
L - lactate cytochrome c oxidoreductase (
EC 1.1.2.3 ) . -
pubmed
Kinetics of
superoxide scavenging by
dismutase enzymes and
manganese mimics determined by electron spin resonance . -
pubmed
Enzymic reactions were studied in which
Cu ,
Zn - SOD ,
Mn - SOD and
Fe - SOD each competed with the spin trap
5,5 - dimethyl-1 - pyrroline 1 - oxide (
DMPO ) for
superoxide anion (
O2- ) at pH 7.8
O2- from dissolved
KO2 (
potassium superoxide ) in
dimethyl sulphoxide was added directly to the
enzyme solutions containing
DMPO . -
pubmed
The results show that , in this competition reaction system , the kinetics of the reactions between the
enzymes and
O2- follow a function y = f [ ( SOD]0.5 ) . -
pubmed
S-1 , determined for
Cu ,
Zn - SOD is approximately an order of magnitude larger than those for
Mn - SOD and
Fe - SOD . -
pubmed
A comparative study of reported
SOD mimics , including
Mn2 + ,
MnO2 - desferrioxamine mesylate (
Desferal ) and
MnO2 - Desferal - ascorbate , was done . -
pubmed
A last generation drug belonging to this family is
pravastatin which differs from the other substances inhibiting the activity of the key enzyme of
cholesterol metabolism ,
HMGCaA reductase , because of certain features of the molecule , such as
hydrophilia and the fact it is already pharmacologically active at the moment of oral administration . -
pubmed
Pravastatin , which is probably a special category of
HMGCoA reductase inhibitor , has shown , in numerous experimental studies and controlled clinical trials , a notable effectiveness in reducing in a highly selective fashion the synthesis of
cholesterol in the liver cells and consequently the number of
cholesterol - rich lipoproteins in the systemic circulation , without also determining significant biologically negative side - effects . -
pubmed
The binding of
A1 to the arm - like projections of the high ( H)- and medium ( M)-molecular - weight neurofilament subunits causes a conformational change of the molecule ( intrafilamentous reaction ) , characterized by an altered migration on
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis (
SDS - PAGE ) . -
pubmed
The possibility that
phosphate groups of the H and M sidearms are involved in binding
A1 ions is discussed with regard to the migration on
SDS - PAGE of dephosphorylated neurofilaments incubated with
A1 compounds and the alteration by
A1 ions of
neurofilament phosphorylation in vitro by the associated
kinase . -
pubmed
Hb Coimbra or alpha 2 beta ( 2)99(G1)Asp----Glu , a newly discovered
highoxygen affinity variant . -
pubmed
We have identified a new high
oxygen affinity
hemoglobin variant in members of a Portuguese family ; it is characterized by an
Asp ---- Glu replacement at codon 99 of the beta chain which is in the alpha 1
beta 2 interface . -
pubmed
The altered functional properties of
Hb Coimbra likely result from the inability to form a
hydrogen bond between beta 99Glu and alpha 42Tyr ; such a bond is formed in deoxy Hb A between the normally occurring beta 99Asp and alpha 42Tyr . -
pubmed
A review of the many variants of the alpha and beta chains identifies primarily
aspartic acid and
glutamic acid residues as being most frequently replaced ; it is speculated that codons
GAC and GAT ( for
Asp ) , and GAG and
GAA ( for
Glu ) are most susceptible to mutational events . -
pubmed
Previous pKa determinations indicated that
histidine 134 , present in the catalytic site of
aspartate transcarbamylase , might be the group involved in the binding of the substrate
carbamyl phosphate and , possibly , in the catalytic efficiency of this
enzyme . -
pubmed
The results obtained show that
histidine 134 is indeed the group of the
enzyme whose deprotonation increases the affinity of the catalytic site for
carbamyl phosphate . -
pubmed
In the wild - type
enzyme this group can be titrated only by those
carbamyl phosphate analogues that bear the
carbonyl group . -
pubmed
In the modified
enzyme the group whose deprotonation increases the catalytic efficiency is still present , indicating that this group is not the
imidazole ring of
histidine 134 ( pKa =
6.3 ) . -
pubmed
Out of 22 medullary respiratory neurones consisting of 14
post - inspiratory and 8 expiratory neurones , 12 neurones were depolarized by
ethanol and 10 neurones were hyperpolarized . -
pubmed
Ethanol depressed the spike activity during stage I expiration of the
post - inspiratory neurones . -
pubmed
The present results demonstrate that
ethanol reduces the expiratory period mainly through the depression of the
post - inspiratory neuronal activity in the bulbar respiratory control mechanism . -
pubmed
Clinical pharmacology of
pinacidil , a prototype for drugs that affect
potassium channels . -
pubmed
The clinical pharmacology of
potassium channel openers has been reviewed using
pinacidil as a prototype drug . -
pubmed
Plasma renin activity , however , was not increased during chronic therapy with
pinacidil monotherapy . -
pubmed
, similar efficacy relative to placebo was noted with a change of
post - dose supine
diastolic blood pressure in the
pinacidil group of 13.5 + /- 0.8 mm Hg and 7.3 + /- 0.9 mm Hg in the placebo group. -
pubmed
Serum creatine kinase ,
aspartate aminotransferase and plasma
progestogen concentrations were measured . -
pubmed
Plasma
PBI concentration , plasma and pituitary
TSH , contents , and hypothalamic
TRH content were measured by RIA in basal and stimulated conditions . -
pubmed
Cross - reactivity of the antisera raised against isolated subunits of the
kinase was assessed by immunodiffusion analysis and by measuring the
cAMP binding and
phosphotransferase activities of the subunits after immunoprecipitation . -
pubmed
Calmodulin and
Ca2 + in normal and transformed cells . -
pubmed
Numerous lines of evidence implicate
calcium and
calmodulin (
CaM ) as regulators of cell growth and functional differentiation . -
pubmed
This paper summarizes the evidence linking
calcium and
CaM to the regulation of cell growth and critically examines the evidence that increases in
CaM levels occur in transformed versus normal cells . -
pubmed
URF1 , encoding an Mr 13500 polypeptide at genome coordinate 17 , is predominantly conserved in nucleotide and
amino acid sequence , but contains one half as many
arginine amino acids as does
URF1 of
Ad2 . -
pubmed
URF2 , encoding an Mr 13600 protein which lies within the i - leader region , is not well conserved in either nucleotide or
amino acid sequence . -
pubmed
CaP were produced in fibers superfused with a
Na +-free ( substituted by
tetraethylammonium chloride ) high
Ca ++ (
16.2 mM ) Tyrode 's solution ( pH 7.4 or pH 6.0 ) . -
pubmed
It was possible to block low pH , CaP with
verapamil alone , while high
RMP , high pH , CaP could be totally blocked only with a combination of
verapamil plus
tetrodotoxin . -
pubmed
As the blood
glucose content ( indicating experimental
diabetes gravity ) rises , the
aldose reductase activity in the tissues increases , the content of
sorbitol and
fructose ascends . -
pubmed
An increase of
aldose reductase and a decrease in the
sorbitol - dehydrogenase activity occur within the first two weeks after
diabetes induction , which correlates with the maximal rate of
sorbitol and
fructose accumulation . -
pubmed
These pressor responses could be significantly attenuated by pretreatment with the
dopamine D2 receptor antagonist
haloperidol , but not the
D1 receptor antagonist
SCH 23390 . -
pubmed
Chemical stimulation of the VTA , by microinjection of 10 nmol of the
substance P analogue
DiMe - C7 , produced a sustained increase in blood pressure ( max 10 - 15 mmHg ) , which could be completely prevented by pretreatment with
haloperidol . -
pubmed
The
Ca ( 2 +)-independent specific
NOS activity is about 30 % of the
Ca ( 2 +)-dependent microsomal activity . -
pubmed
The microsomal
Ca ( 2 +)-dependent
NOS activity is inhibited by 50 % in the presence of 0.5 mM
aminoguanidine ( AG ) , whereas the
Ca ( 2 +)-independent activity does not respond to this concentration of AG , suggesting that it is not the inducible isoform of
NOS . -
pubmed
Our results indicate that ( I )
NOS activity is present from an early phase of placental development , (
II ) the
NOS activity is of trophoblastic origin , since the primordial placenta is avascular and ( III )
NO - production by the primordial placenta can proceed in the absence of any
Ca ( 2 +)-mobilizing agonist . -
pubmed
Plasma concentrations of
FSH ,
LH ,
progesterone ,
testosterone and
oestradiol-17 beta were also determined by RIAs . -
pubmed
The changes in plasma concentrations of
FSH after injection of antisera to
oestradiol-17 beta (
oestradiol - AS ) and
inhibin (
inhibin - AS ) on the morning of day 2 ( day 1 = day of ovulation ) were also determined . -
pubmed
Plasma concentrations of
oestradiol-17 beta showed three increase phases and these changes differed from those of
inhibin . -
pubmed
During repair of
crush injury , however , the level of
antigen drops concomitant with the ingrowth of regenerating axons , suggesting axonal control of
A-1 antigen expression . -
pubmed
[ 18F ] Benperidol (
[ 18F ] BP ) , a positron - emitting analogue of the
dopaminergic D2 antagonist
benperidol , was evaluated as a radiopharmaceutical for use with positron emission tomography ( PET ) . -
pubmed
[ 18F ] BP bound to
D2 receptors in a reversible manner ; unlabeled
eticlopride displaced
D2 receptor - bound
[ 18F ] BP in vivo . -
pubmed
[ 18F ] BP has advantages over other tracers as a radiopharmaceutical for PET study of central
D2 receptor activity , and can be applied for noninvasive evaluation of the interaction of unlabeled drugs with central
D2 receptor sites . -
pubmed
These results demonstrate that
PF4 and
TGF beta 1 inhibit MK development from CD34 + CB cells by different mechanisms and suggest that
PF4 , unlike
TGF beta 1 , exerts its inhibitory effect on cell growth in a reversible and S phase - specific manner by which it protects stem cells and
MK progenitor cells from
5 - FU cytotoxicity . -
pubmed
NMR characterization of structure , backbone dynamics , and
glutathione binding of the
human macrophage migration inhibitory factor (
MIF ) . -
pubmed
Human macrophage migration inhibitory factor is a 114
amino acid protein that belongs to the family of immunologic
cytokines . -
pubmed
From the total of three
cysteines present in the primary structure of
MIF , none was found to form
disulfide bridges . -
pubmed
The capacity to bind
glutathione was investigated by titration of a uniform
15N - labeled sample and led us to conclude that
MIF has , at best , very low affinity for
glutathione . -
pubmed
OBJECTIVE : To investigate the role of
platelet activating factor (
PAF ) in the
hypoxic pulmonary hypertension . -
pubmed
Discrimination of conformational states of
mitochondrial cytochrome P-450scc by selective modification of several
Lys residues . -
pubmed
Selective
serotonin reuptake inhibitors in
affective disorders --
II . -
pubmed
CB1 message expression in all three brain areas returned to vehicle control levels by day 21 of
Delta9 - THC treatment , a time at which behavioral tolerance has been previously reported . -
pubmed
Comparisons suggests that
CB1 message may be regulated by different
effector systems in each of the three areas during chronic
Delta9 - THC exposure . -
pubmed
However , methylation of the
estrogen receptor - alpha gene (
ERalpha ) promoter blocks the expression of
ERalpha , and thereby can antagonize the regulatory effect of
estrogen on cell proliferation . -
pubmed
Using a screening technique , RLGS - M , we show that alteration in methylation associated with the smooth muscle cell phenotypic switch does not seem to require heightened activity of the
methyltransferase enzyme , and appears to be selective for the
ERalpha and a limited pool of genes whose
CpG island becomes either demethylated or de novo methylated . -
pubmed
contains two
malate dehydrogenase isoforms , a mitochondrial isoenzyme with a high specificity for
oxaloacetate and a glycosomal isozyme that acts on a broad range of substrates ( Uttaro , A. D. , and Opperdoes , F.R. -
pubmed
One isoform behaved as a real
malate dehydrogenase with a high specificity for
oxaloacetate , whereas the other showed no activity with
oxaloacetate but was able to reduce other
oxoacids , such as
phenyl pyruvate ,
2 - oxoisocaproate ,
2 - oxovalerate ,
2 - oxobutyrate ,
2 - oxo-4 - methiolbutyrate , and
pyruvate . -
pubmed
SB202190 , a
p38 - specific inhibitor , inhibits UVB - induced
p38 activation and c -
fos gene expression . -
pubmed
Furthermore ,
SB202190 markedly inhibited UVB - induced
AP-1 DNA binding as determined by mobility shift analyses . -
pubmed
Alanine racemases are ubiquitous , almost uniquely prokaryotic enzymes catalyzing the racemization between
l - and
d - alanine . -
pubmed
Water immersion stress prevents
caerulein - induced pancreatic acinar cell
nf - kappa b activation by attenuating
caerulein - induced intracellular
Ca2 + changes . -
pubmed
NF - kappaB is a proinflammatory
transcription factor activated during
caerulein pancreatitis . -
pubmed
Chelation of intracellular
Ca ( 2 + ) by
1,2 - bis ( 2 - aminophenoxy ) ethane - N , N , N ' , N '- tetraacetate /
acetoxymethyl ester has similar effects to water immersion in preventing caerulein but not
TNF - alpha - induced
NF - kappaB activation in pancreas . -
pubmed
Our findings indicate that , in addition to
Ca ( 2 +)-dependent mechanisms ,
Ca ( 2 +)-independent signaling events also may lead to
NF - kappaB activation in pancreatic acinar cells . -
pubmed
Water immersion stress prevents supramaximal
caerulein - induced
NF - kappaB activation in pancreas in vivo and in vitro by affecting intracellular
Ca ( 2 + ) homeostasis . -
pubmed
The resulting
triacylglycerols were analyzed by gas chromatography (
GC ) with a 65 %
phenyl methyl silicone capillary column and separated on the basis of both
fatty acid composition and regiospecific position . -
pubmed
The results showed that this method compares favorably to the existing methods for analysis of
oleic , petroselinic , and cis - vaccenic fatty acids by
GC with respect to peak separation and accuracy , and it also provides information on the regiospecific distribution of the
fatty acids . -
pubmed
Without the vitamin , membranes may have an abnormally high permeability and they may be subject to degradation by endogenous
phospholipases in vivo , as well as being abnormally susceptible to damage in vitro by
dialuric acid and
hydrogen peroxide . -
pubmed
In contrast , purified
human Pol mu alone could only add three
dGMPs as predicted from the sequence of the template . -
pubmed
The production of the
dGMP ladder at the primer terminus located opposite the
AAF adduct reveals an unusual ability of
Pol mu ( in conjunction with its cofactor ) to perform DNA synthesis from a slipped intermediate containing several unpaired bases . -
pubmed
The differences in
amino acid residues of
HLA - B27 subtypes are minor , but may play role in pathogenesis of
ankylosing spondylitis (
AS ) . -
pubmed
Oral contraceptive use and increased plasma concentration of
C - reactive protein . -
pubmed
We examined , in young adult women , the association between current low dose
oral contraceptive ( OC ) use and plasma levels of
C - reactive protein (
CRP ) , an acute phase reactant predictive of
cardiovascular disease risk . -
pubmed
Mutations of charged
amino acids in or near the transmembrane helices of the second membrane spanning domain differentially affect the substrate specificity and transport activity of the multidrug resistance protein
MRP1 (
ABCC1 ) . -
pubmed
Multidrug resistance protein 1 (
MRP1 ) belongs to the
ATP - binding cassette superfamily of transport proteins . -
pubmed
MRP1 consists of two membrane - spanning domains ( MSD2 and MSD3 ) each followed by a
nucleotide binding domain plus a third NH2 - terminal
MSD1 . -
pubmed
MSD2 contains transmembrane (
TM ) helices 6 through 11 , and previously , we identified two charged residues in TM6 as having important but markedly different roles in
MRP1 transport activity and substrate specificity by characterizing mutants containing nonconservative substitutions of
Lys332 and
Asp336 . -
pubmed
We have now extended these studies and found that the same - charge TM6 mutant K332R , like the nonconservatively substituted
Lys332 mutants , exhibits a selective decrease in
leukotriene C4 ( LTC4 ) transport , associated with substantial changes in both Km and Vmax and LTC4 binding . -
pubmed
However , unlike TM6
Asp336 , wild - type transport activity could be reestablished in these
MRP1 mutants by conservative substitutions . -
pubmed
Decreasing
oxygen concentrations cause strong attenuation of
PEDF release resulting in enhanced
VEGF /
PEDF ratios . -
pubmed
However , it caused a
GDP - inhibition of state 4 respiration and a
GDP - induced re - polarization of the inner mitochondrial membrane in the presence of
fatty acids , in agreement with its expected physiological behavior as an
uncoupling protein (
UCP ) . -
pubmed
Finally , the induction of
uncoupling protein-3 also increased the sensitivity of mitochondria to open the permeability transition pore in response to
calcium . -
pubmed
Consumption of
nitric oxide by endothelial cells : evidence for the involvement of a
NAD ( P ) H - , flavin - and heme - dependent dioxygenase reaction . -
pubmed
All experiments were performed in the presence of
superoxide dismutase to minimize the
peroxynitrite reaction . -
pubmed
Both
NO consumption and
nitrate formation were largely inhibited by the
heme poisons
NaCN and
phenylhydrazine as well as the
flavoenzyme inhibitor
diphenylene iodonium . -
pubmed
We found that : ( 1 ) the ratio between the enrichment of
alanine C3 and that of
glutamate C4 ,
gamma - aminobutyric acid (
GABA )
C2 ,
glutamine C4 , or
aspartate C3 decreased from
pentobarbital to
alphachloralose and
morphine whatever the labeled precursor , indicating a link between metabolic and cerebral activity ; ( 2 ) under
glucose +
[ 3 -( 13 ) C ] lactate infusion ,
alanine C3 and
acetyl - CoA C2 enrichments were higher than that of
lactate C3 , revealing the occurrence of an isotopic dilution of the brain exogenous
lactate ( arising from the blood ) by
lactate from the brain ( endogenous
lactate ) , and that the latter was synthesized from glycolysis in a compartment other than neurons ; and ( 3 ) the contribution of labeled
glucose and
lactate to
acetyl - CoA and
amino acid enrichment indicated that the involvement of blood
glucose relative to that of blood
lactate to brain metabolism was correlated with cerebral activity . -
pubmed
Acetyl - coenzyme A carboxylases (
ACCs ) have crucial roles in
fatty acid metabolism . -
pubmed
Unexpectedly , our structures suggest that
soraphen may bind in the BC dimer interface and inhibit the
BC activity by disrupting the oligomerization of this domain . -
pubmed
Cyclosporine A ,
alpha - lnterferon and
interleukin-2 following chemotherapy with
BCNU ,
DTIC ,
cisplatin , and
tamoxifen : a phase II study in advanced
melanoma . -
pubmed
Preclinical data suggest that one method of inducing
autoimmunity to
tumor is the administration and subsequent withdrawal of
cyclosporine A following chemotherapy and that this effect may be enhanced with
interferon and
interleukin-2 . -
pubmed
Cyclosporine A at 3 mg / kg / day in two divided doses was given on days 4 - 21 ,
alpha - interferon 1 million units / m2 subcutaneously every other day on days 4 - 21 and
interleukin-2 1 million units / m2
BID subcutaneously days 21 - 28 were also given . -
pubmed
Enzyme - catalyzed regio- and enantioselective
ketone reductions . -
pubmed
The resulting
propargylic alcohols can be obtained in either enantiomeric form , since ( R)- and ( S)-specific
oxidoreductases can be applied . -
pubmed
When used in treatment of
postoperative nausea and vomiting , a situation where prolongation of the QTc interval seems to occur , the safety of
5 - hydroxytryptamine type 3 antagonists may not be superior to that of low - dose
droperidol . -
pubmed
Is
relaxin a
calcium transporter / buffer ? -
pubmed
Relaxin inhibits smooth muscle contractions by binding
Ca2 + and making it unavailable to the contractile elements . -
pubmed
In the presence of
relaxin , only 4 % of the
45Ca was on the filter with the
SR as compared to that measured on the filter with
SR alone or in the presence of proteins of similar isoelectric point (
cytochrome C ) and size (
insulin ) . -
pubmed
A peak of
45Ca ( & gt;600 K cpm ) was found with the eluted
relaxin . -
pubmed
No peak of
45Ca was associated with
cytochrome C or
insulin under the same conditions . -
pubmed
The eluted fractions containing
45Ca and
relaxin were combined . -
pubmed
Thus ,
45Ca was not tightly bound to
relaxin or the antibodies knocked the 45Ca off . -
pubmed
Is
relaxin a
calcium transporter or buffer ? -
pubmed
AIM :
Adenosine has many actions potentially useful as adjunct to a cardioplegia . -
pubmed
RESULTS : No cross - reactivity of the
antibody was observed with other
aminoglycosides based on competitive direct ELISA methods , indicating that the antibody is highly specific for
neomycin . -
pubmed
Through this method , the detection limits were estimated to be about 10 ng / ml of
neomycin in
PBS , plasma , and milk . -
pubmed
It hydrolyzed the synthetic substrates containing
arginine residue in the
P1 position but did not hydrolyze synthetic substrates containing other
amino acid residues , including
lysine residue in the
P1 position . -
pubmed
Among the synthetic substrates tested , a substrate of
plasminogen activator (
Pyr - Gly - Arg - MCA ) was hydrolyzed most effectively with the
enzyme ( Km = 5.5 x 10(-6 ) M ) , and
fibrin gel was solubilized via activation of intrinsic
plasminogen to
plasmin with the
enzyme . -
pubmed
The serum
LDL level ,
LDL /
HDL ratio ,
ApoB and
MDA in the Huajiao volatile oil group were significantly reduced , compared with those in model group . -
pubmed
Novel genes for
nitrite reductase and
Amo - related proteins indicate a role of uncultivated mesophilic crenarchaeota in
nitrogen cycling . -
pubmed
The insert encoded a homologue of a
copper - containing
nitrite reductase with an unusual C - terminus that encoded a potential amicyanin - like electron transfer domain as well as two proteins related to subunits of
ammonia monooxygenases or particulate
methane monooxygenases (
AmoAB /
PmoAB ) respectively . -
pubmed
Expression of
nirK and the
amoA - like gene was shown by reverse transcription
polymerase chain reaction ( PCR ) analyses in soil samples , the latter being found at higher levels when the soil was incubated with
ammonia ( measured by quantitative PCR ) . -
pubmed
The molecular design made use of a genetically engineered
chaperonin GroEL bearing , at both entrance parts of its cylindrical cavity ,
cysteine residues , which were functionalized by an
azobenzene derivative to construct photoresponsive mechanical gates ( azo -
GroEL ) . -
pubmed
However , when hosting
azo -
GroEL detected
ATP ( input stimulus 1 ) and UV light ( input stimulus 2 ) at the same time , it quickly released GFP(denat ) to allow its refolding . -
pubmed
Activities of
ribulose bisphosphate carboxylase and
phosphoenolpyruvate carboxylase ( expressed in micromoles
CO ( 2 ) per milligram
protein per hour ) averaged 1.95 and 8.87 for the flower buds , and 28.5 and 3.6 for the leaves , respectively . -
pubmed
Protein - bound
ribulose bisphosphate correlates with deactivation of
ribulose bisphosphate carboxylase in leaves . -
pubmed
Previous reports indicate that
ribulose 1,5 - bisphosphate (
RuBP ) binds very tightly to inactive
ribulose bisphosphate carboxylase (
rubisco ) in vitro . -
pubmed
Therefore , we decided to investigate whether there was evidence for tight binding of
RuBP associated with deactivation of
rubisco in vivo . -
pubmed
Bound
RuBP was significantly correlated with the estimated number of inactive
rubisco sites , with a ratio of about 1:1 . -
pubmed
After a step increase in irradiance ,
rubisco activation increased and total
RuBP increased transiently , but steady levels of both occurred by 10 minutes . -
pubmed
The amount of bound
RuBP decreased with a similar time course to the estimated decrease in inactive
rubisco sites . -
pubmed
Bound
RuBP increased gradually but did so more slowly than the estimated increase in inactive
rubisco sites . -
pubmed
The identification of the twin - pore channel ,
TASK , in pulmonary artery smooth muscle , along with reports that it is inhibited by
hypoxia , raises the possibility that a member of this family of channels acts as an
O2 sensor in pulmonary artery . -
pubmed
Increasing evidence points to the importance of achieving low blood
glucose variability and also a low
hemoglobin A1c ( HbA1c ) to prevent
diabetic late complications . -
pubmed
Our data demonstrate that the nuclear interaction of
Smac /
DIABLO with
Survivin is an important step for suppressing the anti - apoptotic function of
Survivin in
Doc - induced apoptosis . -
pubmed
This suggests that the balance between cellular
Smac /
DIABLO and
Survivin levels could be critical for cellular destiny in
taxane - treated
cancer cells . -
pubmed
Effects of
AM281 , a
cannabinoid antagonist , on circulatory deterioration and
cytokine production in an endotoxin
shock model : comparison with
norepinephrine . -
pubmed
Both
AM281 and NE inhibited endotoxin - induced increases in
cytokine production , with significant differences observed among the three groups at 1 and 3 h after treatment . -
pubmed
CONCLUSIONS : Although NE and
AM281 both prevented endotoxin - induced deterioration of systemic hemodynamics ,
AM281 yielded better preservation of mesenteric blood flow and attenuation of
cytokine production than NE . -
pubmed
PURPOSE :
RPE65 , a membrane - associated protein predominantly expressed in the retinal
pigment epithelium , is the
isomerohydrolase in the
retinoid visual cycle . -
pubmed
Three
cysteine (
Cys ) residues ,
Cys231 ,
Cys329 , and
Cys330 , in
RPE65 have been shown to be palmitoylated and have been suggested to be responsible for its membrane association . -
pubmed
The purpose of this study was to define the role of these
Cys residues in palmitoylation , membrane association , and
isomerohydrolase activity of
RPE65 . -
pubmed
METHODS : The three
Cys residues in
RPE65 were replaced by
Alanine (
Ala ) with site - directed mutagenesis . -
pubmed
RESULTS : Mutation of any single residue of these three
Cys significantly reduced protein levels of
RPE65 . -
pubmed
Similar to wild - type
RPE65 , however , all three single
Cys mutants were predominantly present in the membrane fraction . -
pubmed
Mutations of any one or two of these
Cys substantially weakened the
isomerohydrolase activity of
RPE65 , whereas mutations of all three
Cys ( triple mutant ) completely abolished the enzymatic activity . -
pubmed
The structural distortions induced by the
Cys mutations may be responsible for the mislocalization and decreased
isomerohydrolase activities of
RPE65 . -
pubmed
WNK4 , as
thiazides , shuts off
NaCl reabsorption to stimulate
Na / K exchange . -
pubmed
BACKGROUND :
Prostaglandins modulate
cytokine release though increases in
cAMP , regulating
interleukin-6 and
interleukin-10 . -
pubmed
Ketoprofen inhibits
cyclo - oxygenase activity and hence
prostaglandin production . -
pubmed
AIM : We hypothesized that
ketoprofen would affect release of
IL-6 and
IL-10 and modulate the immune response . -
pubmed
At the end of the experimental period , animals were sacrificed and the assay of the activity of
alkaline phosphatase ,
myeloperoxidase , and antioxidants and the estimation of lipid
peroxides were performed . -
pubmed
burgdorferi genome for a canonical integrin binding motif , the RGD (
Arg - Gly - Asp ) tripeptide , revealed several candidate ligands for
integrins . -
pubmed
The transformation to
amino derivatives decreased at high concentrations of
TNT , indicative of inhibition of microbial
TNT transformation . -
pubmed
However ,
methylprednisolone and
Tac promoted increased expression levels of
Bcl-2 in all groups . -
pubmed
This communication is mediated by
cytokines , growth factors and
prostaglandins and dissecting the role of these signaling molecules might be the key to positively manipulate muscle regeneration in the future . -
pubmed
In addition ,
Rpd3p interacted directly with chromatin at boundary regions to deacetylate histone
H4 at
lysine 5 and at
lysine 12 . -
pubmed
Either the mutation of histone
H4 at
lysine 5 or a decrease in the
histone acetyltransferase (
HAT ) activity of
Esa1p abrogated the silencing phenotype associated with
rpd3 mutation , suggesting a novel role for the
H4 amino terminus in
Rpd3p - mediated heterochromatin boundary regulation . -
pubmed
A 6 - mer photocontrolled
oligonucleotide as an effective
telomerase inhibitor . -
pubmed
The small , photocontrolled
oligonucleotide shows effective
telomerase inhibition , both in vitro and in vivo , only when light irradiated . -
pubmed
Mechanism of
glycoside hydrolysis : A comparative QM / MM molecular dynamics analysis for wild type and Y69F mutant retaining
xylanases . -
pubmed
Two - dimensional potential - of - mean - force calculations based upon molecular dynamics with the AM1 / OPLS method for wild - type BCX with a
p - nitrophenyl xylobioside substrate in water clearly indicates a stepwise mechanism for glycosylation : the rate - determining step is nucleophilic substitution by
Glu78 to form the covalently bonded
enzyme - substrate intermediate without protonation of the leaving group by
Glu172 . -
pubmed
The intracellular
calcium oscillations in response to extracellular
Ca ( 2 + ) were of sinusoidal pattern and mediated by the
phospholipase C /
diacylglycerol /
inositol 1,4,5 - trisphosphate pathway as revealed by a biosensor that detects the accumulation of
diacylglycerol in the plasma membrane . -
pubmed
The intracellular
calcium oscillations in response to
aromatic amino acids were of transient type , that is ,
Ca ( 2 + ) spikes that returned to baseline levels , and required an intact
actin cytoskeleton , a functional
Rho ,
Filamin A and the ion channel
TRPC1 . -
pubmed
The pretreatment of cells with
forsythoside A , adding
forsythoside A post infection of cells , and treatment of virus with
forsythoside A were analysed . -
pubmed
Additional experiment joining
DiI and
synaptophysin ( a pre - synaptic protein ) labeling suggested synaptic sites on dendritic shafts and spines . -
pubmed
Eph
tyrosine kinase receptors and their
ephrin ligands are expressed in most adult stem cell niches and in many types of
tumors . -
pubmed
Optical resolution of
n - butyl D - and
L - lactates using immobilized
lipase catalyst . -
pubmed
n - Butyl D - and
L - lactates (
BuDLa and
BuLLa ) were incubated with immobilized
lipase . -
pubmed
The
enzyme was shown to be specific for the
UDP - Gal donor substrate and required
pyrophosphate in the acceptor substrate . -
pubmed
Anti -
thrombotic effects of selective
estrogen receptor modulator
tamoxifen . -
pubmed
Tamoxifen is a known anti -
cancer drug and established
estrogen receptor modulator . -
pubmed
The mass flux of
PFAAs from stormwater runoff in the Twin Cities ( Minneapolis and St. Paul ,
MN ) metropolitan area is estimated to be about 7.86 kg / year . -
pubmed
falciparum infections acquired in Honduras carried
pfcrt ,
pfmdr1 ,
pfdhps and
pfdhfr alleles associated with
chloroquine ,
amodiaquine and
sulphadoxine -
pyrimethamine sensitivity only . -
pubmed
BACKGROUND : Electrophysiological studies of
L - type Ca2 + channels in isolated vascular smooth muscle cells revealed that depolarization of these cells evoked a transient and a time - independent
Ca2 + current . -
pubmed
The
Ca2 +/Mn2 + ion - pump
PMR1 links elevation of cytosolic
Ca ( 2 + ) levels to
alpha - synuclein toxicity in
Parkinson ' s disease models . -
pubmed
The protein
alpha - synuclein (
alphaSyn ) , the principle toxic effector in
PD , has been shown to interfere with neuronal
Ca ( 2 + ) fluxes , arguing for an involvement of deregulated
Ca ( 2 + ) homeostasis in this neuronal demise . -
pubmed
Here , we identify the
Golgi - resident Ca ( 2 +)/ Mn ( 2 + ) ATPase PMR1 (
plasma membrane - related Ca ( 2 +)- ATPase 1 ) as a phylogenetically conserved mediator of
alphaSyn - driven changes in
Ca ( 2 + ) homeostasis and
cytotoxicity . -
pubmed
In conclusion , the
alphaSyn - driven rise of cytosolic
Ca ( 2 + ) levels is pivotal for its
cytotoxicity and requires
PMR1 . -
pubmed
Insulin sensitivity was assessed with the oral
glucose insulin sensitivity ( OGIS ) index . -
pubmed
Insulin secretion was estimated using three model - based parameters of
insulin secretion ( beta - cell
glucose sensitivity , the potentiation factor ratio , and beta - cell rate sensitivity ) . -
pubmed
After complete digestion of the nucleic acids with the
RNases and
DNases , the newly added DNA - modified with the
dNTP analogs , bonds firmly to the NRNs . -
pubmed
Fibrin clot extension on
zirconia surface for dental implants : a quantitative in vitro study . -
pubmed
The
glucagon - stimulated
C - peptide significantly increased in treated cases compared to controls ( p = 0.036 ) . -
pubmed
This result suggests that truncated
Myo52p lacking cargo , or single
molecules of wild - type
Myo52p with small cargoes , can undergo processive movement along
actin -
Cdc8p cables in vivo . -
pubmed
BACKGROUND :
Artemisinin - based combination therapy (
ACT ) is widely recommended as first - line therapy for
uncomplicated Plasmodium falciparum malaria worldwide . -
pubmed
Central to the formation of tertiary structure in membrane protein folding is the presence of
amino acid sequence motifs ( such as " small - XXX - small " segments ) in the
TM segments that promote interaction - compatible surfaces through which the
TM alpha - helices interact . -
pubmed
In this context , we used
proteolipid protein (
PLP ) , the major protein from central nervous system myelin for which mutant - dependent non - native oligomerization has been implicated in
neurological disorders , to explore the specific effects of
TM boundary residues ( the membrane entry and exit points ) , keying on the secondary structure and self - association of peptides corresponding to the
PLP TM2 alpha - helix ( wild - type sequence 66AFQYVIYGTASFFFLYGALLLAEGF90 ) . -
pubmed
The increase in proteinogenic
amino acid concentrations together with the increase in
glutamate dehydrogenase activity suggests a requirement for
nitrogen metabolism . -
pubmed
After 7 days ,
alizarin red staining ,
alkaline phosphatase (
ALP ) assays , and qRT - PCR were used to assess the mineralization , expression of
ALP , and gene expression (
collagen type 1 and
osteocalcin ) , respectively . -
pubmed
Alizarin red and
alkaline phosphatase staining showed mineralization in all three groups . -
pubmed
At termination , CAN was assessed in the renal tissue by light and electron microscopy ,
TG2 and epsilon(
gamma - glutamyl )- lysine by immunofluorescence , and the urinary epsilon(
gamma - glutamyl )- lysine by high performance liquid chromatography . -
pubmed
Extracellular
TG2 ( glomerulus : 64.55 + - 17.61 versus 2.11 + - 0.17 , P & lt ; 0.001 ; interstitium : 13.72 + - 1.62 versus 3.19 + - 0.44 , P & lt ; 0.001 ) , epsilon(
gamma - glutamyl )- lysine ( glomerulus : 21.74 + - 2.71 versus 1.98 + - 0.37 , P & lt ; 0.01 ; interstitium : 37.96 + - 17.06 versus 0.42 + - 0.11 , P & lt ; 0.05 ) ,
TG2 enzyme activity ( 1.09 + - 0.13 versus 0.41 + - 0.03 nmol / h / mg protein , P & lt ; 0.05 ) ,
TG2 mRNA ( 20 - fold rise ) , and urinary epsilon(
gamma - glutamyl )- lysine ( 534.2 + - 198.4 nmol/24 h versus 57.2 + - 4.1 nmol/24 h , P & lt ; 0.05 ) levels were significantly elevated in the allografts and showed a positive linear correlation with tubulointerstitial
fibrosis . -
pubmed
CONCLUSION :
CAN was associated with upregulation of renal
TG2 pathway , which has a potential for pharmacological intervention . -
pubmed
HIF-1alpha signaling activation by
post -
ischemia treatment with
astragaloside IV attenuates
myocardial ischemia -
reperfusion injury . -
pubmed
In this study , we evaluated the effect of
astragaloside IV (
Ast IV )
post -
ischemia treatment on
myocardial ischemia - reperfusion ( IR )
injury (
IRI ) . -
pubmed
Additionally , in cardiomyocytes ,
2 - MeOE2 and
HIF-1alpha siRNA treatment each not only abolished the anti - apoptotic effect of
post -
ischemia treatment with
Ast IV but also reversed the upregulation of
HIF-1alpha and
iNOS expression . -
pubmed
2 - MeOE2 reversed these effects of
Ast IV on IR - injured hearts . -
pubmed
A
NELL-1 isoform with the N - terminal 240
amino acid ( aa ) residues truncated was identified . -
pubmed
Parthenolide induces autophagy via the depletion of
4E - BP1 . -
pubmed
Inhibition of autophagy by knocking out autophagy associated gene
atg5 blocked
PTL - induced apoptosis . -
pubmed
Surprisingly ,
PTL decreased the level of
translation initiation factor eIF4E binding protein 1 (
4E - BP1 ) in correlation with autophagy . -
pubmed
Ectopic expression or shRNA knockdown of
4E - BP1 further verified the effect of
4E - BP1 on
PTL - induced autophagy . -
pubmed
Meanwhile ,
PTL elevated the cellular reactive
oxygen species ( ROS ) which located upstream of the depletion of
4E - BP1 , and contributed to the consequent autophagy . -
pubmed
This study revealed
4E - BP1 as a trigger for
PTL - induced autophagy and may lead to therapeutic strategy to enhance the efficacy of anticancer drugs . -
pubmed
Using data from a randomized controlled trial evaluating computerized
CBT ( CBT4CBT ) , we evaluated treatment response by
COMT genotype , with the a priori hypothesis that
Val carriers would have improved response to computerized delivery of
CBT . -
pubmed
There was a significant treatment condition by genotype interactions for rates of participants attaining 21 or more days of continuous abstinence as well as self - reported percent days of abstinence , suggesting less
cocaine use among
Val carriers when assigned to
CBT compared to standard treatment . -
pubmed
Exploration of possible mechanisms using measures of attentional biased also pointed to greater change over time in these measures among the
Val carriers assigned to
CBT . -
pubmed
CONCLUSION : These are the first data from a randomized controlled trial indicating significant interactions of
COMT polymorphism and behavioral therapy condition on treatment outcome , where
Val carriers appeared to respond particularly well to computerized
CBT . -
pubmed
Our aim was to study the effect of the non - selective
MMP and
TNF - alpha converting enzyme (
TACE ) inhibitor
GM6001 ( 30 mg / kg ) on anastomosis repair in obstructed left colon . -
pubmed
In vitro ,
GM6001 also delayed ( p=0.026 ) epithelialization of denuded intestinal epithelium grown on
type I collagen . -
pubmed
CONCLUSIONS : Non - selective
MMP /
TACE inhibition with
GM6001 increased the anastomotic complications following
colon obstruction . -
pubmed
The effect of
SPRY4 -
IT1 on proliferation was evaluated by
MTT and colony formation assays . -
pubmed
Olive Oil - derived
Oleocanthal as Potent Inhibitor of
Mammalian Target of Rapamycin : Biological Evaluation and Molecular Modeling Studies . -
pubmed
The established anticancer and neuroprotective properties of
oleocanthal combined with the reported role of
mammalian target of rapamycin (
mTOR ) in
cancer and
Alzheimer ' s disease development encouraged us to examine the possibility that
oleocanthal inhibits
mTOR . -
pubmed
To validate this hypothesis , we docked
oleocanthal into the
adenosine triphosphate binding pocket of a close
mTOR protein homologue , namely ,
PI3K - gamma . -
pubmed
Apparently ,
oleocanthal shared nine out of ten critical binding interactions with a potent dual
PIK3 - gamma /
mTOR natural inhibitor . -
pubmed
Subsequent experimental validation indicated that
oleocanthal indeed inhibited the enzymatic activity of
mTOR with an IC50 value of 708 nM. -
pubmed
These results strongly indicate that
mTOR inhibition is at least one of the factors of the reported anticancer and neuroprotective properties of
oleocanthal . -
pubmed
Predictors of
PDB included older age , lower baseline
hemoglobin , lower platelet reactivity on
clopidogrel , and use of chronic oral anticoagulation therapy . -
pubmed
The receptor for
gonadotropin - releasing hormone (
GnRH ) belongs to the
G protein - coupled receptors (
GPCRs ) , and its stimulation activates
extracellular signal - regulated protein kinase (
ERK ) . -
pubmed
We found also that
GnRH induced the cleavage of
ErbB4 . -
pubmed
In the present study , we examined signal transduction for the activation of
ERK and the cleavage of
ErbB4 after
GnRH treatment . -
pubmed
Both
ERK activation and
ErbB4 cleavage were completely inhibited by
YM-254890 , an inhibitor of
Gq / 11 proteins . -
pubmed
Experiments with two types of
PKC inhibitors ,
Go 6976 and
bisindolylmaleimide I , indicated that novel
PKC isoforms but not conventional
PKC isoforms were involved in
ERK activation and
ErbB4 cleavage . -
pubmed
Our experiments indicated that the novel
PKC isoforms activated
protein kinase D (
PKD ) after
GnRH treatment . -
pubmed
After
Cu exposure ,
SOD (
superoxide dismutase ) ,
GPx (
glutathione peroxidase ) ,
GR (
glutathione reductase ) and
GST (
glutathione S - transferase ) activities increased and
CAT (
catalase ) activity decreased in the liver at water at pH 4.5 and 8.0 . -
pubmed
SOD and
CAT activities increased in the white muscle after
Cu exposure at pH 8.0 and
GPx ,
GR and
GST activities decreased after
Cu exposure at pH 4.5 and 8.0 . -
pubmed
The
PAM stabilizes favorable agonist interactions , while
Na (+ ) tends to disrupt agonist binding . -
pubmed
We identified two residues involved in allosteric signal transmission :
Trp 7.35 at the top and
Tyr 7.53 at the bottom of
TM VII . -
pubmed
Gallic acid at 30 mg kg(-1 ) decreased the levels of protein expression of
caspase-3 and
iNOS induced by I / R
injury . -
pubmed
This effect of
gallic acid was mainly mediated by reducing protein expression of
iNOS and
caspase-3 . -
pubmed
Proto - oncogene tyrosine - protein kinase (
Src ) was identified as its membrane target and the dissociation constant ( Kd ) between
Src and
isorhamnetin was 3.81 muM. -
pubmed
Furthermore , anti -
leukemia effects of
isorhamnetin were mediated by
Src through inducing
G2 / M cell cycle arrest . -
pubmed
In this framework , by first processing the time - varying
TRs , a finite number of uncertain but time - constant
TR matrices are obtained . -
pubmed
AIM : The changes of the hypothalamic pituitary adrenal (
HPA ) axis response to a long distance transportation results in increase of
adrenocorticotropic hormone (
ACTH ) and
cortisol levels . -
pubmed
RESULTS : One - way RM - ANOVA showed significant effects of transport on
ACTH changes in Groups B and C and on
cortisol changes in both Groups A and B. -
pubmed
In this study , we hypothesized that
serotonin ( 5 -
HT ) , acting in the retrotrapezoid nucleus / parafacial respiratory group ( RTN / pFRG ) , is able to generate active expiration . -
pubmed
In the
H - H sigma - bond activation of a
dihydrogen molecule by a
nickel ( 0 )- borane complex , the empty p orbital of the
borane moiety interacts with the
H - H sigma bonding MO to form charge transfer (
CT ) from the
dihydrogen molecule to the
borane moiety to accelerate the reaction . -
pubmed
In the B - F sigma - bond activation of
BF3 by a
platinum ( 0 )- bisphosphine complex , the second
BF3 molecule interacts with the F atom that is dissociating from the B atom to stabilize the transition state and product by the
CT from the F atom to the second
BF3 . -
pubmed
The
n - alkanes of all samples had a bimodal distribution with the 1st peak at
n - alkane with 17
carbons (
C17 ) and the 2nd predominant peak at C29 . -
pubmed
SOM received a mixed contribution of plankton ( I ) , low - latitude terrestrial high - grade plants (
II ) and microbial material ( III ) as indicated by isotopic compositions of
long - chain n - alkane . -
pubmed
Tafazzin is involved in the biosynthesis of the unique phospholipid
cardiolipin ( CL ) , which is almost exclusively found in mitochondrial membranes . -
pubmed
Extended dialysis with low
calcium dialysate concentration and
citrate anticoagulation , and administration of
teriparatide led to a further increase in bone - specific
alkaline phosphatase activity and most importantly , resulted in an
activated bone turnover as confirmed by a second bone biopsy 11 weeks later . -
pubmed
PURPOSE : Urine
prostaglandin E2 (
PGE2 ) levels have shown to be a risk factor of
breast cancer , and the use of nonsteroidal anti - inflammatory drugs ( NSAIDs ) is known to be beneficial in preventing
breast cancer risk and/or recurrence with or without
aromatase inhibitors . -
pubmed
We hypothesized that the use of an
aromatase inhibitor triggers the activation of the inflammatory pathway via release of
PGE2 . -
pubmed
Adults with higher
vitamin C levels exhibited lower weight ,
BMI and waist circumference , and better measures of metabolic health , including HbA1c ,
insulin and
triglycerides , all risk factors for
type 2 diabetes . -
pubmed
However , the EPS concentration and
dehydrogenase activity decreased constantly with increasing
AMP dosage . -
pubmed
Treatment with
corticosteroids and
cyclosporine (
CsA ) resulted in a decrease of the methylation level of
GATA3 and
TGF - beta in inactive
BD patients . -
pubmed
Compared with the control and saline groups , IOF of CrPyr increased the plasma
creatine concentration on the day of hatch , and the plasma
pyruvate concentration on the day of hatch and 3 day
post - hatch ( p & lt ; .05 ) . -
pubmed
Enhanced
hemoglobin -
oxygen unloading in migratory salmonids . -
pubmed
Recent findings indicate that some teleost fishes may be able to greatly enhance
hemoglobin -
oxygen ( Hb -
O2 ) unloading at the tissues under conditions that result in
catecholamine release . -
pubmed
Together , these results indicate that a system to enhance
Hb -
O2 unloading may be common among salmonids and may be operational even under routine conditions . -
pubmed
The EGFP expression was attenuated by treatment with an inhibitor for
ERK ,
TGF - beta or reactive
oxygen species ( ROS ) signaling . -
pubmed
Resveratrol protects early
brain injury after
subarachnoid hemorrhage by activating autophagy and inhibiting apoptosis mediated by the
Akt /
mTOR pathway . -
pubmed
In the present study , we examined the effect of
resveratrol on EBI and their potential relationship with the
Akt /
mTOR pathway , autophagy , and apoptosis . -
pubmed
Immunohistochemistry analysis of
beclin-1 ,
LC3 - B treated with
resveratrol alone or in combination with
3 - methyladenine ( autophagy inhibitor ) suggested that
resveratrol induced the autophagy process and the inhibitor blocked the occurrence of autophagy , and also increased the number of terminal deoxynucleotidyl transferase - mediated
digoxigenin - DUTP -
biotin nick - end labeling ( + ) cells . -
pubmed
Taken together , these findings indicate that
resveratrol exerts neuroprotective effects on EBI after
SAH by regulating autophagy and apoptosis mediated by the
Akt /
mTOR pathway . -
pubmed
To test this hypothesis , we evaluated DNA damage along with comprehensive quantification of RNA transcripts ( RNA - seq ) , identification of accessible chromatin (
ATAC - seq ) , and characterization of regions with histone modifications associated with active transcription ( ChIP - seq for acetylation at histone 3
lysine 27 , H3K27ac ) . -
pubmed
At follow - up , no significant differences in cortical or medullary
R2 * values between
AVT and AnonVT were observed , and the
GFR decline was similar in the two groups ( 3.0 vs. 3.3 ml / min per 1.73 m ) . -
pubmed
Ectopic expression of
OsWRKY42 results in reduced expression of cell wall damage and salt stress induced
jasmonic acid biosynthesis and response genes . -
pubmed
CONCLUSION : The results presented here suggest that
OsWRKY42 regulates plant responses to either cell wall damage or salinity stress by acting as a negative regulator of
jasmonic acid mediated responses . -
pubmed
This work provides a rigorous procedure , within the framework of the Reaction Class Transition State Theory ( RC -
TST ) and the Structure - Activity Relationship (
SAR ) , for predicting reliable thermal rate constants on - the - fly for
hydrogen abstraction reactions by
methyl /
ethyl radicals from
Polycyclic Aromatic Hydrocarbons (
PAHs ) in a temperature range of 300 - 3000 K. All necessary RC -
TST parameters were derived from ab initio calculations for a representative set of 36 reactions on which different error analyses and comparisons with available literature data were carried out . -
pubmed
Therefore , the constructed RC -
TST procedure can be confidently used to obtain reliable rate constants on the fly in an attempt to effectively construct detailed kinetic mechanisms for
PAH - related fuels . -
pubmed
According to the sequential extraction procedure (
SEP ) the loosely bounds
Cr ( 90.28 % ) was converted to the relatively strong bound (
Fe - Mn oxides fraction , 92.09 % ) revealed the significant decrease in risk of release and availability of
chromium after immobilization procedure . -
pubmed
The microstructure and microhardness evolution of a
Ti-15(wt.%)
Mo /
TiB metal - matrix composite ( MMC ) during high - pressure torsion (
HPT ) at 400 C was studied . -
pubmed
An increase in dislocation density , a considerable decrease in a grain size in the
bcc Ti matrix , and breaking / rearrangement of the
TiB whiskers were observed during
HPT . -
pubmed
Its versatility lies in the capacity to form eight different inter -
ubiquitin linkages through the seven
lysine residues of
ubiquitin and through its N - terminal
methionine (
M1 ) . -
pubmed
Here , we show that the
histone deacetylase inhibitor ,
trichostatin A (
TSA ) , suppresses
HER2 - overexpressing
breast cancer via upregulation of miR-146a and the resultant repression of its oncogenic targets ,
interleukin-1 receptor - associated kinase 1 and the chemokine receptor
CXCR4 . -
pubmed
Consequently ,
TSA synergizes with
lapatinib , a
tyrosine kinase inhibitor of
HER2 , to suppress
breast cancer in vitro and in rodent models . -
pubmed
Targeting
lactate dehydrogenase - A promotes
docetaxel - induced
cytotoxicity predominantly in castration - resistant
prostate cancer cells . -
pubmed
LDH controls the conversion of
pyruvate to
lactate , with
LDH - A , one of the predominant isoforms of
LDH , controlling this metabolic process . -
pubmed
Sodium oxamate (
SO ) was used as a specific
LDH - A inhibitor . -
pubmed
Cell cycle progression and apoptotic inducibility were evaluated by flow cytometry using
propidium iodide and
Annexin V staining . -
pubmed
LDH expression was strongly associated with
DOC sensitivity in PC cells . -
pubmed
The results strongly indicated that
LDH - A serves an important role in
DOC resistance in advanced
PC cells and inhibition of
LDH - A expression promotes susceptibility to
DOC , particularly in
CRPC cells . -
pubmed
Differences of
cyclin - dependent kinase 4 / 6 inhibitor ,
palbociclib and
abemaciclib , in
breast cancer . -
pubmed
Both
palbociclib and
abemaciclib are , oral , highly selective inhibitors of
cyclin - dependent kinase 4 and 6 , which are proteins involved in cell differentiation and growth . -
pubmed
In pivotal phase III trials ( PALOMA and MONARCH ) , they demonstrated a significant improvement in median progression - free survival in combination with a nonsteroidal
aromatase inhibitor in the first - line , and with a
fulvestrant in the second - line in
hormone receptor - positive and
HER2 - negative metastatic
breast cancer , respectively . -
pubmed
Both
palbociclib and
abemaciclib were approved , however ,
ribociclib , the third
cyclin - dependent kinase 4 / 6 inhibitor , has not been approved in Japan . -
pubmed
In contrast , additional benefits from
abemaciclib in combination with nonsteroidal
aromatase inhibitor or
fulvestrant seem to have a relationship with
visceral disease ,
liver metastasis , primary resistant to endocrine therapy , and short treatment - free interval . -
pubmed
Filtrate analysis after
NaOH ,
NH4OH and
H2SO4 pretreatments yielded release of soluble reducing
sugars (
SRS ) in range of ~0.17 - 0.44 % , ~0.38 - 0.75 % and ~2.9 - 8.4 % respectively . -
pubmed
Release of soluble components other than
SRS , majorly reported to be soluble lignins , were observed highest for
NaOH followed by
H2SO4 and
NH4OH pretreatments . -
pubmed
Application of commercial
cellulase increased raw SCB saccharification from 1.93 % to 38.84 % , 25.56 % and 9.61 % after
NaOH ,
H2SO4 and
NH4OH pretreatments . -
pubmed
The Tx100 - only protocol retained 1.5 times more
collagen and 2.5 times more
sGAG than the livers decellularized with Tx100 +
SDS . -
pubmed
X - ray diffraction studies confirmed that complexes [ 1]-[4 ] feature a
theophyllinato ligand coordinated to the metal center in the rarely observed , chelating fashion via the N7 and
O1 atoms . -
pubmed
We aimed to evaluate the additive and prognostic value of pre - treatment
2 -[ 18F ] fluoro-2 - deoxy - d - glucose positron emission tomography / computed tomography (
2 -[ 18F ] FDG PET /
CT ) in the staging and management of ACa compared to magnetic resonance imaging ( MRI ) . -
pubmed
Pre - treatment
2 -[ 18F ] FDG PET /
CT studies and MRI reports were compared considering the primary
tumor , pelvic lymph nodes , and
metastatic lesions . -
pubmed
The impact of
2 -[ 18F ] FDG PET /
CT in the management and its prognostic value , using maximum standardized uptake value ( SUVmax ) , were assessed . -
pubmed
Insulin clearance has recently been highlighted as a fundamental aspect of
glucose metabolism , as it has been hypothesized that its impairment could be related to an increased risk of developing
type 2 diabetes . -
pubmed
The most common
PCP - related NACDG allergens for both sexes were
methylisothiazolinone(MI ) (
MPCP:28.8 % ,
FPCP:21.5 % ) , fragrance mix I ( 22.3 % , 20.1 % ) , balsam of Peru ( 18.5 % ,
14.1 % ) ,
quaternium-15 (
16.1 % , 12.3 % ) , and
paraphenylenediamine ( 11.5 % , 11.1 % ) . -
pubmed
The
bd quinol oxidase with a higher binding affinity for
O2 than the
aa 3 oxidase is known to play an important role in aerobic respiration under
oxygen - limiting conditions . -
pubmed
Above - 31 cm depth in the core ,
TN ,
TP , TOC , C / N , and
delta13C increased significantly , indicating that eutrophication has occurred since the 1980s . -
pubmed
The binding site for
rabbit skeletal alpha - tropomyosin on
troponin - T has been localized to the
cyanogen bromide fragment ,
CB2 ( residues 71 - 151 ) , using affinity chromatography . -
pubmed
It is concluded that the helical region of
CB2 , and perhaps the
carboxyl side chains of
aspartic and glutamic acids , may be involved in binding over a limited surface area of the double - stranded coiled
coil of
alpha - tropomyosin . -
pubmed
Mu - chain protein GLI is a pentameric molecule with an
amino - terminal deletion comprising 130 residues . -
pubmed
The half -
cysteine residue ( position 140 ) which forms the H - L
disulfide bridge in normal
IgM is present . -
pubmed
It is concluded that after
dbcAMP stimulation , Sertoli cells produce 29 - kDa
inhibin and the alpha - subunit precursor proteins
pro - alpha C and
alpha N. -
pubmed
The production of the alpha - subunit precursor in addition to
inhibin provides an explanation for the decrease in the
inhibin B : I ratio following
dbcAMP stimulation of Sertoli cells in culture . -
pubmed
The seed
proteins are rich in most of the essential
amino acids , and they are deficient only in
cystine and
threonine , when compared to the WHO / FAO requirement pattern . -
pubmed
The antinutritional factors ( total free
phenols ,
tannins ,
trypsin inhibitor activity and haemagglutinating activity ) were also investigated . -
pubmed
In pancreatic islets the bulk of
phosphoinositide - specific
phospholipase C (
PI - PLC ) activity was cytosolic . -
pubmed
The soluble enzyme was activated by submicromolar concentrations of
Ca2 + , independent of
calmodulin . -
pubmed
All four
pyridine nucleotides stimulated
PI - PLC . -
pubmed
The
diglyceride lipase inhibitor ,
RHC 80267 , and the
cyclooxygenase inhibitor ,
indomethacin , had no effect on
PI - PLC activity . -
pubmed
Platelet adhesivity and aggregation induced by
ADP ,
adrenaline and
collagen , and
malondialdehyde production , are tests in which
dipyridamole and
aspirin shows a potentiation synergism . -
pubmed
Trunk plasma
corticosterone ( B ) and plasma T , and B production by
collagenase - isolated adrenocortical cells incubated briefly ( 2 hr ) with or without steroidogenic agents were measured by radioimmunoassay . -
pubmed
Orchiectomy with implantation of the inert
sterol ,
cholesterol ( hereafter referred to as orchiectomy ) , did not alter plasma B concentrations and did not affect basal cellular B production or cellular B production induced by a maximal steroidogenic concentration of
ACTH or that maximally supported by
25 - hydroxycholesterol . -
pubmed
Low - implant doses of
A4 ( 1 - cm implant ) and T ( 0.3 - cm implant ) , that maintained comb growth , lowered plasma B concentrations by 24 - 42 % , whereas a high - implant dose of T ( 3 - cm implant ) and all implant doses of
DHT had no effect on plasma B concentrations . -
pubmed
In contrast ,
androgen replacement consistently suppressed basal and maximal
ACTH - induced cellular B production regardless of the type of
androgen . -
pubmed
The method is based on the fact that the
anti - factor VIII antibodies were predominantly of the
IgG4 subclass ;
anti - human IgG4 antibodies were covalently linked to
agarose and large amounts of
anti - factor VIII antibodies can be eliminated . -
pubmed
Xylosyltransferase activity was determined in synovial fluid and serum by a radiochemical method , based on the incorporation of
[ 14C ] xylose from
UDP -[ 14C ] xylose into an exogenous acceptor protein . -
pubmed
BCNU and
CDDP were least active in the monolayer assay , whereas
HME showed both the lowest ID90 and steepest slope using this technique . -
pubmed
The
MK-0787 /
MK-0791 is a combination of
imipenem ( a
carbapenem antibiotic ) and
cilastatin sodium ( a
dehydro peptidase - I inhibitor ) in a 1 to 1 ratio , which produces a higher urinary recovery of
imipenem than
imipenem alone . -
pubmed
The determination of the saturation of
serum albumin with
bilirubin . -
pubmed
Maternal and umbilical cord serum were checked for
copper and
zinc by the proton - induced X - ray emission ( PIXE ) method ; maternal serum protein ,
hemoglobin and
estriol were determined as well . -
pubmed
Relaxation to
histamine was potentiated by inhibition of
histamine H1 - receptors with
chlorpheniramine . -
pubmed
Effects of
Ca2 + and
Mg2 + on the
actin - activated ATP hydrolysis by phosphorylated
heavy meromyosin from arterial smooth muscle . -
pubmed
Actin - activated
ATP hydrolysis by phosphorylated
arterial myosin is
Ca2 + -dependent at
Mg2 + concentrations which allow
myosin to bind 2 mol of
Ca2 + per mol ( S. Chacko and A. Rosenfeld ( 1982 ) Proc . -
pubmed
The possible effects of
myosin filament formation and conformational change involving the rod portion of the
myosin molecule on
actin -
myosin interaction and
actin - activated ATP hydrolysis was eliminated by utilizing soluble
heavy meromyosin (
HMM ) . -
pubmed
Actin - activated
ATPase activity of the phosphorylated
HMM was measured either at constant
Mg2 + and variable
Ca2 + or at constant
Ca2 + and variable
Mg2 + concentrations . -
pubmed
At constant ( 0.05 M ) ionic strength and pCa 5 , the
actin - activated
ATPase activity increased until the free
Mg2 + reached between 0.5 - 1 mM ; this was followed by a decrease in activity to a very low level at 7 mM free
Mg2 + . -
pubmed
Removal of
Ca2 + at 1 mM free
Mg2 + lowered the
actin - activated
ATPase activity ( 40 - 60 % inhibition ) . -
pubmed
These experiments demonstrated that
Mg2 + and
Ca2 + had a direct effect on
actin - activated ATP hydrolysis . -
pubmed
It is concluded that combined treatment with
glucocorticoid and
beta 2 - adrenoceptor agonist may result in a synergistic interaction on severe
airway obstruction without significant influence on cardiovascular system . -
pubmed
Thymopoietin ( originally termed
TP-5 and now called
thymopentin ) is a synthetic pentapeptide that can reproduce the biological activity of the 49
amino acid thymic hormone
thymopoietin . -
pubmed
Treatment of plasma with specific
enzyme inhibitors followed by incubation with
thymopentin or
thymopoietin confirmed that
serine protease and
aminopeptidase M - like enzymes are responsible for the rapid inactivation of
thymopentin in plasma , as measured by the electromyographic response . -
pubmed
During the first few turnovers of the
enzyme catalytic cycle , when an appreciable amount of E approximately P is still present , the observed heat is much smaller than the heat expected from the amount of
ATP hydrolyzed . -
pubmed
IgG - titers in all ELISA assays were between 10 - fold to 100 - fold higher when
formalin - heat inactivated preparations were employed as compared to
phenol - heat inactivated . -
pubmed
It is different from the binding fo
Co2 + , which binds to the base and
phosphate of residue
G15 . -
pubmed
Cimetidine in a low dose of 10 mg given intravenously increases significantly the gastric potential difference (
GPD ) without any inhibitory effect on gastric acid secretion . -
pubmed
The increase of
GPD after 200 mg
cimetidine is higher than after 10 mg . -
pubmed
[ Effect of
lincomycin ,
chymotrypsin and their combinations on the splenic plasmacytic reaction and the antibody titer in experimental
staphylococcal infection ] . -
pubmed
Lincomycin and
chymotrypsin were used in doses of 150 and 2 mg / kg respectively . -
pubmed
It was shown that the treatment of the experimental
staphylococcal infection with
lincomycin resulted in decreased proliferation of the plasmatic cells and
antibody formation . -
pubmed
The use of
chymotrypsin in conjunction with
lincomycin lowered the suppressing effect of the latter on the above indices . -
pubmed
We analysed the effects of
morphine microinjections ( 0.2 microliter ) into the subnucleus oralis (
SNO ) , i.e. -
pubmed
This effect was dose - dependent , reversed by
naloxone , and also site - specific to the
SNO since no effect was observed after microinjection of
morphine into areas adjacent to the
SNO . -
pubmed
A hypoplastic cortical plate was induced and histological analysis and
BrdU - immunohistochemistry revealed that the RGD sequence is involved in
neuronal migration and proliferation in the telencephalon during formation of the cortical plate . -
pubmed
The energy provision from
glutamine also plays a role in the process of platelet activation , known to require an extra supply of
ATP , since
thrombin increased the rate of
glutamine utilization in platelets . -
pubmed
Upon cell activation an increase in
aspartate and
CO2 formation was observed and the stimulatory effect on
glutamine oxidation by
thrombin was also maintained in the presence of
glucose . -
pubmed
In this way the regulation of hepatic
glutaminase expression is similar to that seen for other enzymes involved in hepatic
amino acid catabolism but differs markedly from that of renal
glutaminase , in which changes in transcription rate are not observed and alterations of mRNA turnover are the principle mechanism of long - term regulation . -
pubmed
Gonadal function was monitored by
serum LH ,
FSH ,
testosterone ,
SHBG and
inhibin levels . -
pubmed
In
haemophiliacs with less than 1 %
factor VIII level , rise was maximum ( 3 - 6 folds ) ; mean
factor - VIII level at 7th and 14th day of
danazol therapy was 2.3 + /- 0.6 % and 4.8 + /- 1.1 % , respectively . -
pubmed
There was a significant positive correlation between the increases in
Cx-43 transcripts and the increase in the ratio of plasma
estradiol to
progesterone . -
pubmed
Our data demonstrate : 1 ) that transcripts encoding the gap junction protein ,
Cx-43 , are at maximal levels during delivery and that this increase is temporally associated with increases in
Cx-43 protein and the appearance of gap junctions ; and 2 ) that these data , in association with changes in plasma
steroid concentrations , are consistent with myometrial
Cx-43 transcript levels being regulated positively by
estrogen and negatively by
progesterone during pregnancy . -
pubmed
Three putative
cAMP responsive elements ( CRE ) and one putative
TPA responsive element ( TRE ) were identified upstream of the
GC rich region . -
pubmed
The results of this study suggest that corrective intertrochanteric osteotomy is a successful treatment for Ficat and Arlet stage -
II or III disease if the patient has a small or medium lesion ( a combined
necrotic angle of less than 200 degrees ) and is not receiving continuous high doses of
corticosteroids . -
pubmed
SEB was chemically conjugated to
tumor cells using a heterobifunctional cross - linking agent acting through
NH2 and
SH groups . -
pubmed
It is shown that
formylphenyl ester of
ATP is not the substrate in the reaction of elongation catalyzed by
primase . -
pubmed
Similarly ,
interferon alpha-2b did not inhibit the culture of mast cells from
CD34 + bone marrow - derived cells in the presence of
SCF . -
pubmed
Intact
Kat4c and
F ( ab ') 2 fragments were found to augment fMLP - induced oxidation of
1,2,3 - dihydrorhodamine ( oxidant species production ) and
beta 2 integrin - mediated adhesion to
fibrinogen but did not promote
beta 2 integrin - mediated binding of
albumin coated latex beads . -
pubmed
Biochemical and antiproliferative properties of
4 -[ ar ( alk ) ylamino ] pyridopyrimidines , a new chemical class of potent and specific
epidermal growth factor receptor tyrosine kinase inhibitor . -
pubmed
The
tyrosine kinase inhibitors
PD 69896 , 153717 , and
158780 , which belong to the chemical class
4 -[ ar ( alk ) ylamino ] pyridopyrimidines , have been characterized with respect to enzymology , target specificity , and antiproliferative effects in
tumor cells . -
pubmed
PD 158780 reduced clone formation in soft agar of fibroblasts transformed by
EGF ,
EGF receptor , or the
neu oncogene but not
ras or
raf , further demonstrating its high degree of specificity . -
pubmed
The
sechiumin cDNA has 951 nucleotides , encoding a protein with 285
amino acids . -
pubmed
Two
amino acid residues ,
Glu160 and
Arg163 , at the putative active site of
sechiumin , are known to be catalytically active in
ricin and
abrin . -
pubmed
Submicellar
bile salts stimulate
phosphatidylcholine transfer activity of
sterol carrier protein 2 . -
pubmed
To explore a potential role for
sterol carrier protein 2 (
SCP2 , also known as
non - specific lipid transfer protein ) in hepatocellular phospholipid trafficking , we examined the influence of submicellar
bile salt concentrations on
phosphatidylcholine ( PC ) transfer activity of
SCP2 . -
pubmed
Taurine- and
glycine - conjugated
bile salts ( anionic
steroid detergent - like molecules ) , at concentrations well below their critical micellar concentrations , stimulated PC transfer activity of
SCP2 80- to 140 - fold . -
pubmed
Thermodynamic and kinetic analyses of PC transfer support a mechanism in which
bile salts stimulate
SCP2 activity by partitioning into donor vesicles and enhancing membrane association of
SCP2 . -
pubmed
Myocardial blood flow ( MBF ) was measured during
dipyridamole loading and at baseline using positron emission tomography and
13N - ammonia , after which
MVD was calculated before and after lipid - lowering therapy . -
pubmed
There was a significant relationship between the percent change of total
cholesterol and the percent change of
MVD before and after lipid - lowering therapy ( r=-0 . -
pubmed
Gas chromatography (
GC ) is used for the analysis of
trans - fatty acids in partially hydrogenated vegetable oils . -
pubmed
The isolated
trans - monoenes are quantitatively analyzed by capillary
GC . -
pubmed
The technique of argentation TLC with
GC analysis of isolated
methyl esters is highly reproducible with 4.8 % variation ( i.e. -
pubmed
The mutation is located in the
S4 voltage sensor of domain
IV , predicting substitution of
histidine for the fifth of eight arginines ( R1460H in
hSkm1 ) . -
pubmed
However , there was no increase in persistent
sodium current as observed for
SCN4A mutations causing
myotonia or periodic
paralysis . -
pubmed
The present review deals with various aspects of such regulations , emphasizing the role of
calcium / calmodulin in activating
AC1 and
AC8 in the central nervous system , the potential inhibitory effect of
calcium on
AC5 and
AC6 , and the changes in the expression pattern of the isoforms during development . -
pubmed
Spectroscopy of polymer / drug formulations processed with supercritical fluids : in situ
ATR - IR and Raman study of impregnation of
ibuprofen into
PVP . -
pubmed
ATR - IR spectroscopy has also revealed specific interactions between the
C = O groups of
PVP and
CO2 . -
pubmed
Fludarabine (
FLU , a
fluorinated purine analog ) and
idarubicin (
IDA , a
DNA - topoisomerase II poison ) are frequently used in
cancer chemotherapy . -
pubmed
Changes in respiratory rate ( RR ) , heart rate (
HR ) ,
oxygen saturation ( SpO(2 ) ) , Respiratory Distress Assessment Instrument ( RDAI ) , and activity levels were assessed at 20 minutes intervals at times -20 , 0 , 20 , 40 , and 60 minutes around treatment . -
pubmed
casseliflavus and 47/47
vancomycin susceptible enterococci were negative for the
vanA and
vanB genes in both PCR assays . -
pubmed
Abietadiene synthase from
grand fir catalyzes two sequential , mechanistically distinct cyclizations , of
geranylgeranyl diphosphate and of
copalyl diphosphate , in the formation of a mixture of
abietadiene isomers as the committed step of
diterpenoid resin acid biosynthesis . -
pubmed
To obtain an insight into fluorophores formed in
proteins during lipid peroxidation , a
lysine residue analogue (
N ( alpha )- hippuryllysine ) was exposed to autoxidation of
linoleic acid catalyzed by
iron ( III )- EDTA and
L - ascorbic acid . -
pubmed
The reaction predominantly produced two fluorescent products ,
N , N '- bis [ 5 -( N - benzoylglycylamino )- 5 - carboxypentyl ]- 2 - hydroxy-2 - pentyl-3 - imino - l,2 - dihydropyrrole (
II ) and
N , N '- bis [ 5 -( N - benzoylglycylamino )- 5 - carboxypentyl ]- 2 - hydroxy-2 -( 7 - carboxyheptyl )- 3 - imino-1,2 - dihydropyrolle ( I ) . -
pubmed
Studies on
serum oxidase (
ceruloplasmin ) inhibition by
tryptophan metabolities . -
pubmed
The appearance of
antibody of higher specific activity was demonstrated to be a specific response to the
antigen which was reinjected . -
pubmed
In term of
carbohydrate (
CHO ) , the ratio of slow digestible starch (
SDS ) and resistant starch (
RS ) to total
CHO for two biscuits ( & gt ; 60 % ) were higher than bread ( 18.3 % ) , although the
sugar content were also higher . -
pubmed
Contrast to GI and II value of
glucose ( as 100 % ) , the GI and
II for bread was 96.2 % and 105.2 % , respectively . -
pubmed
Glucocorticoid (
GC ) sensitivity was measured by
dexamethasone (
DEX ) inhibition of lipopolysaccharide ( LPS)-induced
interleukin-6 (
IL-6 ) and
tumor necrosis factor - alpha (
TNF - alpha ) production in whole blood . -
pubmed
We compared
trysin - digested protein samples desalted by ZipTip(C18 ) reverse - phase microcolumns with on - plate washing of peptides deposited either on paraffin - coated plates (
PCP ) ,
Teflon - based AnchorChip plates , or stainless steel plates , before analysis by matrix - assisted laser desorption / ionization - time of flight - mass spectrometry ( MALDI -
TOF -
MS ) . -
pubmed
We demonstrate that the method of washing of
protein samples on
paraffin - coated plates provides an easy , reproducible , inexpensive , and high - throughput alternative to ZipTip(C18)-based purification of
protein prior to MALDI -
TOF -
MS analysis . -
pubmed
Oral ( OGTTs ) and intravenous
glucose tolerance tests ( IVGTTs ) and a euglycaemic hyperinsulinaemic
clamp were performed before and at 6 and 12 months of treatment . -
pubmed
In OGTTs , serum
insulin and
C - peptide concentrations 2 h after the oral
glucose load were increased ( P<0.001 and P=0.05 respectively ) at 12 months of treatment with TIB , but no changes in the areas under the curve ( AUC ) of
insulin or
C - peptide were observed . -
pubmed
CONCLUSIONS : Despite the impairment in peripheral
insulin sensitivity ,
TIB treatment had a neutral effect on
glucose tolerance , possibly due to a compensatory decrease in endogenous
glucose production . -
pubmed
The increased demand on
insulin induced by ED , due to both a stimulatory effect on pancreatic beta cells and increased
insulin metabolism , may explain the slightly detrimental effect on
glucose tolerance with this treatment . -
pubmed
Oximes - induced reactivation of
rat brain acetylcholinesterase inhibited by VX agent . -
pubmed
Insulin - secreting beta - cells , located within the pancreatic islets of Langerhans , are excitable cells that produce regular bursts of action potentials when stimulated by
glucose . -
pubmed
Neonates of & lt;33 weeks
GA with
MUL showed significantly increased
PTS at term . -
pubmed
In a systematic screen of brain - expressed genes from this region in 210 families with
XLMR , we identified seven different mutations in
JARID1C , including one frameshift mutation and two nonsense mutations that introduce premature stop codons , as well as four missense mutations that alter evolutionarily conserved
amino acids . -
pubmed
[ The dynamics of the domains of the
IP3 - binding site of the
inositol-1,4,5 - triphosphate - sensitive calcium channel , induced by
inositol-1,4,5 - triphosphate and
calcium ] . -
pubmed
The dynamics of the
inositol-1,4,5 - triphosphate - sensitive calcium channel after binding of
inositol-1,4,5 - triphosphate and
Ca2 + was analyzed by the Monte Carlo minimization technique . -
pubmed
In the study we explored ( 1 ) the effect of the length of
acyl chains of lipids (
C16:1 - C24:1 ) and
alkanes (
C6 - C16 ) , ( 2 ) the role of the
carbonyl group of lipids , and ( 3 ) the effect of molecular structure of the sarcoplasmic reticulum membrane on TeCP partitioning . -
pubmed
present in vivo evidence for 2 important functions of
CHGA : the regulation of
catecholamine - containing dense - core chromaffin granule biogenesis in the adrenal gland and the control of blood pressure . -
pubmed
These results suggest a coupled relationship between
CHGA - mediated chromaffin granule biogenesis , necessary for
catecholamine storage , and
catestatin - induced inhibition of cholinergic - stimulated
catecholamine release , which regulates autonomic control of blood pressure . -
pubmed
A quantitative structure - activity relationship study on some
aromatic / heterocyclic sulfonamides and their charged derivatives acting as
carbonic anhydrase inhibitors . -
pubmed
A quantitative structure - activity relationship ( QSAR ) study is made on a series of
aromatic / heterocyclic sulfonamides and their charged derivatives acting as
carbonic anhydrase (
CA ) inhibitors . -
pubmed
2000 ; 43 : 292 ) for the selective inhibition of
CAs -- sulfonamides generally do not discriminate between different
CA isozymes and hence exhibit many undesirable side effects when used as drugs against a particular disease . -
pubmed
Acute
estradiol significantly increased the number of surviving
CA1 neurons , assessed either at 7 days after
ischemia or after the completion of behavioral testing 9 days after
ischemia . -
pubmed
In contrast , chronic
estradiol did not reduce
CA1 cell
death 9 days after
ischemia . -
pubmed
In an in vitro study , we also examined the potential role of
butyrate in modifying the generation of the profibrotic
cytokine transforming growth factor - beta (
TGF - beta1 ) by renal epithelial cells . -
pubmed
In vitro work demonstrated that exposure of renal epithelial cells to elevated concentrations of
butyrate suppressed both basal and stimulated
TGF - beta1 synthesis . -
pubmed
The action of
butyrate was mediated by suppression of the
extracellular signal - regulated kinase /
mitogen - activated protein kinase signalling pathway . -
pubmed
In addition ,
butyrate exposures reduced the response of renal epithelial cells to
TGF - beta1 as assessed by
luciferase activity of a TGF - beta - responsive reporter construct . -
pubmed
In conclusion , the data demonstrate that dietary supplementation with SUPERGU increased serum
butyrate , which at least in vitro has beneficial effects on renal pro - fibrotic
cytokine generation . -
pubmed
Whereas all treatments affected levels of
GH mRNA , only
CORT ,
GHRH , and
SRIF significantly altered
GHRH - R mRNA levels . -
pubmed
Furthermore , there was no change in the colocalization of
EphA2 and
VE - cadherin at cell - cell adhesion sites when
EphA2 was phosphorylated on
tyrosine residues . -
pubmed
The inhibitors of the mitochondrial
ATPase ,
oligomycin and
azide , and also
rotenone and
antimycin A , were all without effect . -
pubmed
Our results indicate that elemental semiconductors from group 14 of the periodic table can be made to adopt mesostructured forms such as
MSU -
Ge-1 , which features two three - dimensional labyrinthine tunnels obeying Ia3d space group symmetry and separated by a continuous
germanium minimal surface that is otherwise amorphous . -
pubmed
The depletion of platelet
GSH and the increase in
S - glutathionylated proteins occurred at concentrations of
disulfiram that inhibited
platelet aggregation , suggesting that protein S - glutathionylation is involved in the inhibition of
platelet aggregation caused by
disulfiram . -
pubmed
BACKGROUND : Few data are currently available investigating
neurosteroids (
NS ) in
Alzheimer ' s disease (
AD ) . -
pubmed
subtilis mutator
strain (
mutS ,
mutM ,
mutY ) , two thermo - adaptants were isolated from the
groEL substituted
strain at 52 degrees C. They contained novel
amino acid alterations in their
ATP binding motif ( T93I ) and the inter - monomer contact ( R285H ) region of
GroEL . -
pubmed
The presented simulation algorithm allows to model two experimentally observed instances of the
metal oxide /
electrolyte interface : one possessing a common intersection point (
CIP ) at pH =
PZC and the other one with
CIP not equal PZC . -
pubmed
Olmesartan prevented vascular
eNOS dimer disruption or the downregulation of
dihydrofolate reductase (
DHFR ) more than
pravastatin , whereas
Akt phosphorylation was enhanced by
pravastatin but not
olmesartan , indicating differential pleiotropic effects between
olmesartan and
pravastatin . -
pubmed
Moreover ,
pravastatin enhanced the increase in
eNOS activity by
olmesartan , being associated with additive effects of
pravastatin on phosphorylation of
Akt and
eNOS . -
pubmed
Density functional theory ( B3LYP ) and ab initio (
MP2 ) methods with the 6 - 31G(d , p ) basis set are used to study the mechanisms for the hydrolysis of
N -( 2 - oxo-1,2 - dihydro - pyrimidinyl ) formamide (
PFA ) in the gas phase . -
pubmed
pretreatment with the
mu - opioid receptor antagonist
beta - funaltrexamine , the
kappa - opioid receptor antagonist
nor - binaltorphimine and the
delta - opioid receptor antagonist
naltrindole . -
pubmed
pretreatment with antisera against the endogenous kappa - opioid peptides
dynorphin A , dynorphin B and alpha - neo - endorphin ; and the endogenous delta - opioid peptide [
Leu(5)]enkephalin . -
pubmed
The present results suggest that the spinal antinociception induced by the
mu - opioid receptor selective peptide
amidino - TAPA is mediated in part by the release of endogenous opioid peptides in the spinal cord , which is caused by the direct stimulation of
mu - opioid receptors . -
pubmed
Light is thought to be absorbed by mitochondrial chromophores leading to an increase in
adenosine triphosphate (
ATP ) , reactive
oxygen species and/or
cyclic AMP production and consequent gene transcription via activation of
transcription factors . -
pubmed
A natural material ,
collagen , was then immobilized onto the nanofiber surface by
N -( 3 - dimethylaminopropyl )- N '- ethylcarbodiimide hydrochloride (
EDC)/
N - Hydroxysuccinimide (
NHS ) activation process . -
pubmed
The stability of the
cTnI level was best in the serum ,
lithium - heparin plasma , and
lithium - heparin whole blood samples ( n = 10 , decrease & lt ; 10 % in 24 hours at +20 ( degrees ) C and at +4 ( degrees ) C. There was no remarkable influence of hemodialysis on the
cTnI release . -
pubmed
A new series of neutral
5 - substituted 4 - anilinoquinazolines as potent , orally active inhibitors of
erbB2 receptor tyrosine kinase . -
pubmed
Starting from initial lead 1 containing a basic 5 - substituent , optimisation of the
glycolamide - derived neutral 5 - substituent led to potent inhibitors of
erbB2 with good pharmacokinetics . -
pubmed
RESULTS : Based on receiver operating characteristic ( ROC ) curve analysis , the CCCR was only marginally better , as judged by the area under curve ( AUC = 0.923 + /- 0.021 (
SE ) ) , than the 24 - h
calcium /
creatinine excretion ratio ( AUC = 0.903 + /- 0.027 ) and the 24 - h
calcium excretion ( AUC = 0.876 + /- 0.029 ) . -
pubmed
Glial - gonadotrophin hormone (
GnRH ) neurone interactions in the median eminence and the control of
GnRH secretion . -
pubmed
This function , initially described for astrocytes apposing magnocellular neurones , has been more recently characterised for neurones secreting
gonadotrophin hormone - releasing hormone (
GnRH ) . -
pubmed
Last , an isotopic labeling technique applied to the
rhodamine 6 G ( R6G )/ silver system serves as an additional proof of the existence of single - molecule
SERS and explores the dynamical features of this process . -
pubmed
The
ST6Gal - I glycosyltransferase , which adds alpha2 - 6 - linked
sialic acids to glycoproteins , is overexpressed in colon
adenocarcinoma , and enzyme activity is correlated with
tumor cell invasiveness . -
pubmed
Correspondingly ,
collagen binding is reduced by enzymatic removal of cell surface
sialic acids from
ras - expressors with high
ST6Gal - I levels ( i.e. -
pubmed
Improvement of charge - transfer indices for multifunctional
amino acids : application to
lysozyme . -
pubmed
The use of
CT / valence
CT indices { G(k ) , J(k ) , G(k)(V ) , J(k)(V ) } gives limited results for modelling
amino acid pI. -
pubmed
The fitting line for 21
amino acids is used to estimate
lysozyme pI replacing ( 1 + Delta n / n(T ) ) with ( M + Delta n)/n(T ) . -
pubmed
The increase in [
Ca(2+)](i ) of both cultures was inhibited by
pyridoxalphosphate-6 - azophenyl-2 ' , 4 '- disulphonic acid , a
P2 receptor antagonist . -
pubmed
U73122 ,
phospholipase C inhibitor and
Thapsigargin , a
calcium - pump inhibitor , significantly inhibited the increase in [
Ca ( 2 +)]( i ) at both stages . -
pubmed
CbpF shows a new modular structure composed both of consensus and non - consensus
choline - binding repeats , distributed along its length , which markedly alter its shape , charge distribution and binding ability , and organizing the protein into two well - defined modules . -
pubmed
Effect of different application depths of
mitomycin - C in deep sclerectomy with
collagen implant : a randomized controlled trial . -
pubmed
PURPOSE : To evaluate effects of
mitomycin - C (
MMC ) in deep sclerectomy with
collagen implant applied under the superficial scleral flap or under the deep scleral flap . -
pubmed
Randomized controlled trials ( RCTs ) on the treatment of
FMS with
GPT and
PGB were analyzed . -
pubmed
Intermittent
hypoxia - mediated plasticity of acute
O2 sensing requires altered red - ox regulation by
HIF-1 and
HIF-2 . -
pubmed
This article provides a brief review of recent studies addressing the effects of chronic intermittent
hypoxia (
IH ) on acute
O2 sensing in carotid bodies ( CBs ) and adrenal medullary chromaffin cells ( AMCs ) and the underlying mechanisms . -
pubmed
Thus , the studies with chronic IH suggest novel , hitherto uncharacterized , roles for
HIF-1 and
HIF-2 in regulating red - ox status leading to plasticity of
O2 sensing in CBs and AMCs . -
pubmed
Mg2+/
Ca2 + cation binding cycle of
guanylyl cyclase activating proteins (
GCAPs ): role in regulation of
photoreceptor guanylyl cyclase . -
pubmed
Suppression of
Ca ( 2 + ) influx through the
cGMP channel by light activates
retinal guanylyl cyclase through
guanylyl cyclase activating proteins (
GCAPs ) and thus expedites photoreceptors recovery from excitation and restores their
light sensitivity . -
pubmed
GCAP1 and
GCAP2 , two ubiquitous among vertebrate species isoforms of
GCAPs that activate
retGC during rod response to light , are myristoylated
Ca ( 2 +)/ Mg ( 2 +)-binding proteins of the EF - hand superfamily . -
pubmed
In the metal binding EF - hands of
GCAP1 , different point mutations can selectively block binding of
Ca ( 2 + ) or both
Ca ( 2 + ) and
Mg ( 2 + ) altogether . -
pubmed
Activation of
retGC at low
Ca ( 2 + ) ( light adaptation ) or its inhibition at high
Ca ( 2 + ) ( dark adaptation ) follows a cycle of
Ca ( 2 +)/ Mg ( 2 + ) exchange in
GCAPs , rather than release of
Ca ( 2 + ) and its binding by
apo - GCAPs . -
pubmed
The
Mg ( 2 + ) binding in two of the EF - hands controls docking of
GCAP1 with
retGC1 in the conditions of light adaptation and is essential for activation of
retGC . -
pubmed
Mg ( 2 + ) binding in a C - terminal EF - hand contributes to neither
retGC1 docking with the
cyclase nor its subsequent activation in the light , but is specifically required for switching the cyclase off in the conditions of dark adaptation by binding
Ca ( 2 + ) . -
pubmed
The
Mg ( 2 +)/ Ca ( 2 + ) exchange in
GCAP1 and 2 operates within different range of intracellular
Ca ( 2 + ) concentrations and provides a two - step activation of the
cyclase during rod recovery . -
pubmed
Additionally ,
alcohol - dependent patients were interviewed with the
Alcohol Use Disorders Identification Test ( AUDIT ) and were subtyped according to Lesch 's
Alcohol Typology (
LAT ) . -
pubmed
There were no significant gender differences in the AUDIT and
LAT between
alcohol - dependent men and women . -
pubmed
Because
VEGF and its downstream mediator
nitric oxide have a well - established cardioprotective role , however , it can be argued that the beneficial effects of
VEGF antagonism in the eye may come at the cost of adverse systemic effects , particularly
myocardial infarction and
stroke . -
pubmed
CONCLUSION : Results suggest the combination of a
PBI and a peer - delivered
BMI is an appropriate and efficacious way to reduce drinking and related consequences among individuals who initiated drinking earlier in adolescence and are at an increased risk of experiencing
alcohol problems . -
pubmed
OxyVita - zero - link polymerized
hemoglobin ( OxyVita Hb ) is a novel generation
hemoglobin based
oxygen carrier ( HBOC ) . -
pubmed
The enzyme
homogentisate phytyltransferase (
HPT ) performs the first committed step of the
vitamin E biosynthetic pathway . -
pubmed
The cDNA , designated as
LsHPT , was 1,670 bp long containing an open reading frame ( ORF ) of 1,185 bp which encoded a protein of 395
amino acids . -
pubmed
Transient expression of
LsHPT via agroinfiltration resulted in 9 - fold increase in
LsHPT mRNA level and nearly 18 - fold enhancement in
alpha - tocopherol content compared with the negative controls . -
pubmed
Our research showed that concentrations of
lactate ,
phosphates and pH value
post - mortem depended on the perimortal situations . -
pubmed
Next , we use these novel inhibitors to pinpoint the time of
Hog1 action during recovery from G(1 ) checkpoint arrest , providing further evidence for a specific role of
Hog1 in regulating cell cycle resumption during
arsenite stress . -
pubmed
RESULTS : We have developed an improved kinetic model featured with the incorporation of
butyryl - phosphate , inclusion of net effects of complex metabolic regulations , and quantification of endogenous
enzyme activity variations caused by these regulations . -
pubmed
Through parameter perturbation analysis , it was found that
butyrate kinase has large and positive influence on
butanol production while
CoA transferase has negative effect on
butanol production , suggesting that
butyrate kinase has more efficiency in converting
butyrate to
butanol than
CoA transferase . -
pubmed
This clinical evidence leads to the hypothesis that
GSH conjugation catalyzed by
GSTT1 and
GSTM1 has a role in the elimination of reactive metabolites of
troglitazone . -
pubmed
It is of interest that one of the reactive metabolites with a
quinone structure was predominantly conjugated with
GSH by
GSTM1 . -
pubmed
Thus , we demonstrated that the
GST isoforms contributed differently to the
GSH conjugation of individual reactive metabolites of
troglitazone , and
GSTM1 is the most important
GST isoform in the
GSH conjugation of a specific reactive metabolite produced from the cytotoxic ,
quinone - form metabolite of
troglitazone . -
pubmed
Finally , cerebral
phospho -
ERK expression was measured by western blot . -
pubmed
Furthermore , the elevated
caspase-3 activity seen following
burn injury was remarkably reduced by high dose
gelsolin treatment along with down - regulation of
phospho -
ERK expression . -
pubmed
Idraparinux is a polymethylated synthetic
pentasaccharide that binds to
antithrombin with high affinity . -
pubmed
By studying the expression of
ER specific hallmarks , such as
GRP78 ,
GRP94 and
GADD153 /
CHOP , we found a possible pivotal role of
ER signaling modulation in the crosstalk between autophagy and apoptosis induced by
cisPt . -
pubmed
To the best of our knowledge , this is the first demonstration that
taurine enhances autophagic protection against apoptosis by reducing
ER stress , thus making it possible to develop new strategies to reduce severe
cisPt - induced side - effects such as
nephrotoxicity . -
pubmed
Treg and
IL-6 levels decreased following
GEM monochemotherapy ,
IL-17A levels decreased after GEMOX , and
IL-6 levels were reduced subsequent to BEV+
CAPE+RT treatment . -
pubmed
A regulatory path associated with
X - linked intellectual disability and
epilepsy links
KDM5C to the
polyalanine expansions in
ARX . -
pubmed
Polyalanine ( polyA)-expansion - encoding mutations of
aristaless - related homeobox (
ARX ) cause a spectrum of
X - linked ID ( XLID ) diseases and
chronic epilepsy , including
infantile spasms . -
pubmed
In this study , the effects of
Rho - inactivating toxins on the concentrations of cellular
F - actin were investigated using the
rhodamine -
phalloidin - based
F - actin ELISA . -
pubmed
The immunosuppressive agent
mizoribine monophosphate is an inhibitor of the
human RNA capping enzyme . -
pubmed
Mizoribine monophosphate (
MZP ) is a specific inhibitor of the cellular
inosine-5 '- monophosphate dehydrogenase (
IMPDH ) , the enzyme catalyzing the rate - limiting step of de novo
guanine nucleotide biosynthesis . -
pubmed
MZP is a highly potent antagonistic inhibitor of
IMPDH that blocks the proliferation of T and B lymphocytes that use the de novo pathway of
guanine nucleotide synthesis almost exclusively . -
pubmed
In the present study , we investigated the ability of
MZP to directly inhibit the
human RNA capping enzyme (
HCE ) , a protein harboring both
RNA 5 '- triphosphatase and
RNA guanylyltransferase activities . -
pubmed
Our biochemical studies provide the first insight that
MZP can inhibit the formation of the RNA cap structure catalyzed by
HCE . -
pubmed
In the presence of
MZP , the
RNA 5 '- triphosphatase activity appears to be relatively unaffected while the
RNA guanylyltransferase activity is inhibited , indicating that the
RNA guanylyltransferase activity is the main target of
MZP inhibition . -
pubmed
Kinetic studies reveal that
MZP is a non - competitive inhibitor that likely targets an allosteric site on
HCE . -
pubmed
RESULTS : On the basis of this evidence we selected
Rifampicin , a bacterial
RNA polymerase inhibitor , and then AMBER 12 was used to simulate the behavior of the RPO -
Rifampicin complex after a set of 5000 ps and up to 300 K in water . -
pubmed
The second group contained 40 teeth , restored with pure
titanium casting
post and core . -
pubmed
pylori strains 26695 and
SS1 and followed a sequential linear assembly of the alpha-1,6 - glucan ,
dd - heptan , and O - chain components . -
pubmed
Cat ,
Gpx7 ,
Gad1 ,
Hspa12A ,
Foxn1 ,
adenosine A1 receptor ,
Ca ( 2 + ) adaptor and homeostasis proteins ,
Cacnb4 ,
Atp2b2 , anti - apoptotic
Bcl-2 gene members ,
intracellular trafficking protein ,
Grasp and
suppressor of cytokine signaling (
Socs3 ) ] . -
pubmed
The observed differential transcriptional responses may contribute to early - life
seizure - induced pre - conditioning and neuroprotection by reducing
glutamate receptor - mediated
Ca ( 2 + ) permeability of the hippocampus and redirecting inflammatory and apoptotic pathways . -
pubmed
Potassium iodide , systemic and intralesional
corticosteroids , non - steroidal anti - inflammatory drugs ,
salicylates ,
tumor necrosis factor - alpha inhibitors ,
hydroxychloroquine ,
colchicine , and
dapsone are other treatment options available , but some of these drugs are contraindicated in pregnancy while others are considered safe . -
pubmed
The
tumors were diagnosed as
HCC by typical imaging findings and elevated serum
alpha - fetoprotein (
AFP , 1,825.0 ng / ml ) and protein induced by
vitamin K absence
II (
PIVKA II , 3,043 mAU / ml ) . -
pubmed
The effect of
Sirt3 on cell proliferation was observed with
Edu (
5 - ethynyl - 2 '- deoxyuridine ) kit after
Sirt3 silencing by small interference RNA . -
pubmed
Interestingly , unlike
MECP ,
EFCP and
AFCP did not have any effect at lower concentrations ( & lt;40microg / ml ) but induced pore opening at 60microg / ml , 80microg/ ml , 100microg / ml and 120microg / ml by 1.6 , 3.1 , 12.7 , 16.7folds , respectively for
EFCP and 1.4 , 5.4 , 7 and 10 folds respectively , for AFCP . -
pubmed
The effects of
magnesium sulfate on
placental vascular endothelial growth factor expression in
preeclampsia . -
pubmed
OBJECTIVE : To evaluate the effect of
magnesium sulfate (
MgSO ( 4 ) ) on placental expression levels of
vascular endothelial growth factor (
VEGF ) . -
pubmed
Exposure of
preeclamptic placentas to
MgSO ( 4 ) resulted in decreased
VEGF levels by the amnion ( 57 + - 26 pg / g protein ) , as compared with the control group ( 153 + - 62 pg / g protein ) ( p & lt ; 0.05 ) . -
pubmed
On the other hand ,
MgSO ( 4 ) significantly increased
VEGF levels by the placental villous and the decidua ( 58 + - 15 pg / g protein , 70 + - 29 pg / g protein ; respectively ) , as compared with the control group ( 29 + - 11 pg / g protein , 33 + - 14 pg / g protein ; respectively ) ( p & lt ; 0.01 , p & lt ; 0.05 ; respectively ) . -
pubmed
Exposure to
MgSO ( 4 ) did not affect
VEGF levels in normotensive placentas . -
pubmed
Perfusion with
MgSO ( 4 ) affects
VEGF expression differently by preeclamptic and normotensive placentas . -
pubmed
Increased production of placental
VEGF in
preeclampsia may play a role in the therapeutic action of
MgSO ( 4 ) . -
pubmed
This study was performed to investigate the effects of
acteoside on various cellular functions such as , intracellular
Ca ( 2 + ) mobilization ,
phospholipase C activity , and exocytosis induced by melittin . -
pubmed
Melittin ( 0.1 - 1 muM ) dose - dependently increased intracellular
Ca ( 2 + ) mobilization in the presence of extracellular
Ca ( 2 + ) , but was not affected by 1 muM
U73122 , a specific
PLC inhibitor . -
pubmed
Correlating cation ordering and voltage fade in a
lithium -
manganese - rich
lithium - ion battery cathode
oxide : a joint magnetic susceptibility and
TEM study . -
pubmed
[ Effect of
integrin beta8 on
TGF - beta1 activation in astrocytes with
oxygen glucose deprivation ] . -
pubmed
OBJECTIVE : To study the effect of
beta8 expression on
transforming growth factor beta1(
TGF - beta1 ) activation in astrocytes with
oxygen glucose deprivation (
OGD ) . -
pubmed
Online HILIC / mass spectrometry (
MS ) with a quadrupole time - of - flight mass spectrometer provides accurate mass identifications of the separated ,
2 - AB - labeled N - glycans . -
pubmed
The use of
TFA provided distinct advantages over conventional
ammonium formate as a mobile phase additive , such as , optimal elution order for
sialylated N - glycans , reproducible chromatographic profiles , and matching total ion current chromatograms , as well as minimal signal splitting , analyte adduction , and fragmentation during HILIC /
MS , maximizing sensitivity for trace - level species . -
pubmed
Application of this HILIC /
MS method to the detailed characterization of a marketed therapeutic mAb ,
Rituxan ( ) , is described . -
pubmed
Inhibition of autophagy potentiates the efficacy of
Gli inhibitor
GANT-61 in
MYCN - amplified
neuroblastoma cells . -
pubmed
However ,
NB with
MYCN amplification is less sensitive to
GANT-61 than those without
MYCN amplification . -
pubmed
METHODS : Autophagic process was examined in two
MYCN amplified and two
MYCN non - amplified
NB cells treated with
GANT-61 . -
pubmed
RESULTS : Here we show that
GANT-61 enhanced autophagy in
MYCN amplified
NB cells . -
pubmed
Both an autophagic inhibitor
3 - methyladenine (
3 - MA ) and genetic disruption of
ATG5 or
ATG7 expression suppressed
GANT-61 induced autophagy and significantly increased apoptotic cell
death , whereas pre - treatment with an apoptotic inhibitor ,
Z - VAD - FMK , rescued
GANT-61 induced cell
death and had no effect on the autophagic process . -
pubmed
In the other hand ,
GANT-61 barely induced autophagy in
MYCN non - amplified
NB cells , but overexpression of
MYCN in
MYCN non - amplified
NB cells recapitulated
GANT-61 induced autophagy seen in
MYCN amplified
NB cells , suggesting that the level of
GANT-61 induced autophagy in
NB cells is related to
MYCN expression level in cells . -
pubmed
To define the functional role of
DES1 and the role that the
sulfide molecule may play in the regulation of physiological processes in plants , we studied the localization of the expression of this gene at the tissue level . -
pubmed
An increase in
DES1 expression in response to
ABA was recently demonstrated ; in the present work , we observe that in vitro
auxin treatment significantly repressed the expression of
DES1 . -
pubmed
Since aggregation due to contaminating
nucleic acids may have occurred during the sample concentration step , anion - exchange column chromatography was performed immediately after affinity chromatography , to separate the intact
AdipoR1Delta88 from the aggregating species . -
pubmed
The purified
AdipoR1Delta88 and
AdipoR2Delta99 proteins were characterized by thermostability assays with
7 - diethylamino-3 -( 4 - maleimidophenyl )- 4 - methyl coumarin , thin layer chromatography of bound lipids , and surface plasmon resonance analysis of ligand binding , demonstrating their structural integrities . -
pubmed
Differential effects of
oxycodone ,
hydrocodone , and
morphine on the responses of
D2 / D3 dopamine receptors . -
pubmed
Here , we report that
TUBB3 directly interacted with another
Netrin-1 receptor
Down syndrome cell adhesion
molecule (
DSCAM ) and
Netrin-1 increased this interaction in primary neurons . -
pubmed
It is suggested that
Ca2 + signal transduction and redox seem to be involved in differentiation , while
PGA and
EIF are associated with the division of cambium initials for
COR - induced secondary laticifer differentiation in the rubber tree . -
pubmed
The immobilization relies on the ultra high affinity of
calmodulin to N5A mutant
CBP ( RWKKNFIAVSAANRFKKIS ) in presence of
calcium ( KD~2 pM ) , which can be reversed by
EDTA allowing controlled " capture and release " of the specific binders . -
pubmed
The MC calculated photon fluence in detector arrays was used for image reconstruction of a simple water phantom as well as
polyacrylamide polymer gel (
PAG ) used for radiation therapy . -
pubmed
Lck association with
CRIF1 in Jurkat mitochondria was confirmed biochemically and by microscopy , but did not lead to
CRIF1 tyrosine phosphorylation . -
pubmed
AIM : Decreasing
cholesterol levels in Western populations is the main reason for decreasing mortality due to
coronary heart disease . -
pubmed
Blocking the
PI3K -
Akt pathway by
LY294002 led to an enhancement of
IL-12 production . -
pubmed
PI3 K signalling pathway appears to be involved in the decreased
IL-12 secretion by
HBeAg treated DCs . -
pubmed
By analyzing different variables of the formulation ,
sodium hydrogen carbonate is introduced as pH modifier to improve the release of GLZ , while ethyl cellulose acts as release retardant to reduce the burst release phase of
MTF . -
pubmed
Data also shows EOP tablet is able to release
MTF and
GLZ in a synchronized and sustained manner both in vitro and in vivo . -
pubmed
Expression of both the
gr1 and
gr2 genes was induced by reactive
oxygen species . -
pubmed
Additionally , when the anterograde neurotracer
biotinylated dextran amine (
BDA ) was deposited in the dmVMH subsequent stimulation of the dmVMH produced
BDA - labelled neural fibres with terminal boutons surrounding
Fos - labelled neurons in the dPAG , suggesting synaptic contacts between dmVMH and dPAG neurons for eliciting panic - like behavioural responses . -
pubmed
The commonest clinically significant major resistance mutations associated with the highest levels of reduced susceptibility or virological response to the relevant
Nucleoside Reverse Transcriptase Inhibitor ( NRTI ) were ; the Non -
thymidine analogue mutations ( Non - TAMS ) M184V-20.7 % and K65R-8.0 % ; and the TAMs M41L and K70R ( both 8.0 % ) . -
pubmed
Of the 7 patients with major
PI DRMs , five had high level resistance to
ritonavir boosted
Lopinavir and
Atazanavir , with
Darunavir as the only susceptible
PI tested . -
pubmed
An extension to the build - up method and an analysis of the
post - exercise recovery period that can increase
CO2 generation rates are presented . -
pubmed
RESULTS : Supplementation of mothers with 4400 IU of
vitamin D3 resulted in an enhanced broad - spectrum proinflammatory
cytokine response of cord blood mononuclear cells to innate and mitogenic stimuli ( P = .0009 ) , with an average 1.7- to 2.1 - fold increase in levels of several
proinflammatory cytokines (
GM - CSF ,
IFN - gamma ,
IL-1beta ,
IL-6 , and
IL-8 ) across stimuli , a higher gene expression level of
TLR2 ( P = .02 ) and
TLR9 ( P = .02 ) , a greater than 4 - fold increase in
IL-17A ( P = .03 ) production after polyclonal T - cell stimulation , and an enhanced
IL-10 response of cord blood mononuclear cells to
dexamethasone treatment in culture ( P = .018 ) . -
pubmed
Selenomethionine was subsequently introduced into the crystals to obtain the phases for the
MRF DBD structure . -
pubmed
Data from Biolog
carbon source utilization tests and
nucleotide sequence data from 16S and
gyrB loci suggested that both pathogenic and non - pathogenic
strains were related to , yet distinct from , previously described Xanthomonas species . -
pubmed
Dextran
sodium sulfate was given either on
post - operative day 9 or day 24 to evaluate immune responses in the intestine both immediately following surgery and after a period of healing . -
pubmed
Catecholamine doses and SAPS -
II scores decreased significantly . -
pubmed
Selenium (
Se ) is an essential trace element that is involved in numerous biological processes in the form of a selenoprotein such as
iodothyronine deiodinase ( DIO ) . -
pubmed
Increases in free T4 levels during
Se deficiency were reduced with
Se supplementation ; however , neither free T3 nor
TSH levels were affected . -
pubmed
X - linked hypophosphatemia (
XLH ) , the most common form of
inheritable rickets , is caused by inactivation of
phosphate - regulating gene with homologies to
endopeptidases on the X chromosome ( PHEX ) and leads to
fibroblast growth factor ( FGF ) 23 - dependent renal inorganic
phosphate ( Pi ) wasting . -
pubmed
Fifty - nine elite runners and triathletes were assessed for the following measurements : Th1 , Th2 and lymphocyte phenotyping and plasma levels of
cortisol ,
chemokines ,
inflammatory cytokines and specific
immunoglobulin E (
IgE ) . -
pubmed
Ivacaftor is a drug used to treat
cystic fibrosis (
CF ) patients carrying specific gating
CFTR mutations . -
pubmed
In an independent and larger cohort , we aimed to evaluate whether
SLC26A9 variants contribute to the variability of the lung phenotype and if they influence the lung response to
ivacaftor . -
pubmed
Other
SLC26A9 SNPs also contributed to the
ivacaftor - response . -
pubmed
Interindividual variability in lung response to
ivacaftor is associated with
SLC26A9 variants in French
CF patients . -
pubmed
This high yielding , alpha - selective process was found to be independent on the nature or configuration
[ ( R )- C ( sp3 )- F ,
( S )- C ( sp3 )- F ] of the substituent at
C2 . -
pubmed
With plasma
glucose concentrations clamped between 6 and 9 nmol / l ,
GLP-1 or saline was infused for 240 min during and after a meal . -
pubmed
This review is a short survey of the atomistic computational investigations of K+ and
Na+ ion channels , focusing on
KcsA and several voltage - gated channels from the
KV and
NaV families , which have garnered many successes and engendered several long - standing controversies regarding the nature of their structure - function relationship . -
pubmed
Incidence of
VAs , serum levels of
malondialdehyde (
MDA ) and
superoxide dismutase (
SOD ) , expression of
connexin (
Cx43 ) ,
Bcl-2 ,
Bax ,
caspase-3 ,
tumor necrosis factor ( TNF )- alpha ,
interleukin-6 (
IL-6 ) , and
high mobility group box ( HMGB ) 1 were compared . -
pubmed
Compared with the SO group ,
Cx43 ,
SOD , and
Bcl-2 were decreased ,
MDA ,
Bax ,
caspase-3 ,
TNF - alpha ,
IL-6 , and
HMGB1 were increased in the
MI group , and all the alterations were significantly restrained in the
LOMD+
AMI group . -
pubmed
The charge distribution and electrostatic potential (
ESP ) of 3D - GN@
Cu0 further illustrated the distribution and transfer of electrons on the catalyst surface , which predicted a micro - electrolysis - promoted Fenton - like reaction mechanism . -
pubmed
Nicorandil , the first clinically applied
ATP - sensitive K + channel ( K+ATP ) opener with nitrate property , has demonstrated cardioprotective effects in patients with multiples of heart diseases . -
pubmed
CRISPR and stable gene transfer showed that
PGC1alpha knockout tubular cells were sensitized to the genotoxic stressor
cisplatin whereas transgenic cells were protected . -
pubmed
PGC1alpha not only counteracted this effect but also raised basal mitophagy , as did the downstream mediator
nicotinamide adenine dinucleotide (
NAD + ) . -
pubmed
PGC1alpha did not consistently affect known autophagy pathways modulated by
cisplatin . -
pubmed
These results uncover an unexpected effect of
cisplatin on mitophagy and
PGC1alpha 's exquisite reliance on lysosomes for kidney protection . -
pubmed
Nine
ACE types , childhood relationships and six health outcomes ( early
alcohol initiation , problem
alcohol use , smoking , drug use , therapy , suicide attempt ) were explored . -
pubmed
Serum albumin saturation test based on non - esterified
fatty acids imbalance for clinical employment . -
pubmed
Since physiologically
fatty acids are transported bound to
albumin , we propose here a simple and cheap test that consists of
albumin isoelectric focusing determination to measure the potential systemic
NEFA cytotoxicity . -
pubmed
The
albumin saturation test can point out
serum albumin -
NEFA saturation through a cheap assay that could be performed by any care facility . -
pubmed
In the group that used
DRT plus NPWT , the mean
DRT take was 99.8 % , with a mean maturation time of 16.68days and the mean of the skin graft take rate was 89.1 % . -
pubmed
Also , the mean Q computed in the left
PA ( QLPA - n ) is within 10 % of the mean QLPA - e. Finally , the compliance cMPA - n is computed to be 27 % lower than cMPA - e , while the
cLPA - n is computed to be 20.4 % lower than
cLPA - e. Importantly , the
PA shows significant intra - vascular variation in compliance , with the
MPA showing higher overall compliance compared to the
LPA ( 3.5 - 4 times ) . -
pubmed
RESULTS :
NO2 exposure for 75 days was associated with a significant decrease in FEV100 / FVC% , an increased in functional residual capacity ( FRC ) , and histologic evidence of
emphysema , moreover ;
NO2 exposure led to elevated
triglyceride (
TG ) , red blood cell ( RBC ) ,
hemoglobin (
HGB ) , and hematocrit ( HCT ) levels . -
pubmed
NaHS could alleviate the
PF decline as possible indicated by an elevation of
HDL - C levels and leukocyte . -
pubmed
coli O157 : H7 - infected macrophage ; and these effects of
DHEA were heightened when the cells were pre - treated with
p38 MAPK inhibitor
SB203580 . -
pubmed
RESULTS : Multivariate analyses revealed that for participants aged 8 to 11 , daily
sodium intake was significantly and positively associated with femoral neck
BMD ( B = 0.9 x 10- 5 , p = 0.031 ) ; in particular , subgroup analyses by sex found that in male participants aged 8 - 11 , daily total
cholesterol intake ( B = 5.3 x 10- 5 , p = 0.030 ) and
calcium intake ( B = - 2.0 x 10- 5 , p & lt ; 0.05 ) were significantly associated with femoral neck
BMD in a positive and negative manner , respectively , but neither were observed in female participants of this age group . -
pubmed
In contrast , daily intakes of
vitamin D and
magnesium were significantly and positively associated with femoral neck
BMD in female participants aged 8 - 11 ( B = 246.8 x 10- 5 and 16.3 x 10- 5 , p = 0.017 and 0.033 , respectively ) . -
pubmed
For participants aged 16 to 19 , daily total fat intake was significantly and negatively associated with femoral neck
BMD ( B = - 58 x 10- 5 , p = 0.048 ) ; further stratification by sex found that
magnesium and
sodium intakes were significantly and positively associated with femoral neck
BMD only in females of this age group ( B = 26.9 x 10- 5 and 2.1 x 10- 5 , respectively ; both p & lt ; 0.05 ) . -
pubmed
fossilis did not increase
urea excretion or accumulation during 6 days of
ammonia exposure , and lacked detectable
carbamoyl phosphate synthetase I or III activity in its liver . -
pubmed
Exposure to 30 mmol l-1
NH4Cl led to significant increases in the transcript levels of
nkaalpha1b ( on day 6 ) and
nkaalpha1c1 ( on day 1 and 3 ) as compared with the control . -
pubmed
fossilis is attributable partly to its ability to actively excrete
ammonia with the aid of
Nka . -
pubmed
Design , synthesis , and molecular docking study of new
monastrol analogues as
kinesin spindle protein inhibitors . -
pubmed
A series of 18
3,4 - dihydropyrimidine analogues bearing a
1,2 - methylenedioxybenzene component at position 4 with diverse side chains at positions 5 and 6 was designed and synthesized as inhibitors of the
Eg5 kinesin enzyme . -
pubmed
A molecular docking study was performed for some demonstrative compounds as well as
monastrol docked into the allosteric binding site of the
kinesin spindle protein . -
pubmed
This systematic review (
SR ) proposed the following question : Can the
toxicity of
TiO2 nanoparticles be evaluated in a 3D skin model ? -
pubmed
In this
SR , 7 articles were selected , and it was concluded that the 3D skin model has shown to be promising to evaluate the
toxicity of
TiO2 NPs . -
pubmed
A strategy to limit this degradation was developed and tested for model ECDs based on a sidechain - modified
poly ( 3,4 - ethylene dioxythiophene ) (
PEDOT ) derivative and
Prussian blue (
PB ) . -
pubmed
Copper is essential for many metabolic processes but must be sequestrated by
copper chaperones . -
pubmed
It is well known that plant
copper chaperones regulate various physiological processes . -
pubmed
However , functions of
copper chaperones in plant nucleus remain largely unknown . -
pubmed
CCP harbors a classical MXCXXC
Cu binding site (
CBS ) at its N terminus and a nuclear localization signal ( NLS ) at the C terminus . -
pubmed
Collectively , our results have identified a putative nuclear
copper chaperone required for plant immunity and provided evidence for potential function of
Cu in the
SA pathway . -
pubmed
,
angiotensin II ,
endothelin 1 ,
aldosterone ) impairs the balance between vasoconstrictors and vasodilators and , in turn , detrimentally impacts vascular and erectile structures . -
pubmed
Widely studied mechanisms of action , such as complex I inhibition leading to
AMPK activation , have only been observed in the context of supra - pharmacological ( > 1mM )
metformin concentrations which do not occur in the clinical setting . -
pubmed
Efficacy measures included changes from baseline in
glycated hemoglobin (
A1C ) , fasting plasma
glucose ( FPG ) , and
body weight . -
pubmed
Venous Air Embolism Identified by
18F - Fluorocholine Dual - Phase PET /
CT . -
pubmed
F - FCH PET /
CT with dual time point was performed , acquiring immediately and 60 minutes after
F - FCH administration . -
pubmed
A method for the determination of
oxoglutarate binding to the external
face of the inner membrane is described . -
pubmed
Recoveries of individual subunits were 75 % (
S1 ) , 70 % (
S2 ) , greater than 90 % (
S3 ) , greater than 90 % (
S4 ) , and 50 % (
S5 ) , as judged by
SDS - PAGE and
amino acid analysis . -
pubmed
Juvenile GM2 gangliosidosis caused by substitution of
histidine for
arginine at position 499 or 504 of the alpha - subunit of
beta - hexosaminidase . -
pubmed
Response of
rat liver glutaminase to
magnesium ion . -
pubmed
The activity of
rat liver glutaminase from sedimented fractions of freeze - thawed mitochondria is strongly affected by variation in the
Mg2 + concentration within the approximate physiological range of activators . -
pubmed
A rise in the
Mg2 + concentration stimulates
glutaminase by increasing the apparent affinity of the enzyme for its positive modifier
phosphate . -
pubmed
The result is an increase in the apparent affinity of the
enzyme for
glutamine . -
pubmed
With the addition of 4 mM
Mg2 + the S0.5 of
glutaminase for
glutamine decreased from 24 to 13 mM at pH 7.1 , 14 to 9.6 mM at pH 7.4 , and remained unchanged at 8.2 mM at pH 7.7 . -
pubmed
Since
Mg2 + stimulates
glutaminase , as does a rise in pH ( Szweda , L.I. -
pubmed
264 , 15357 - 15360 ) , by increasing the apparent affinity of the
enzyme for
phosphate , it reduces the inhibitory effect of a decrease in pH and/or
phosphate concentration over a physiologically relevant range . -
pubmed
Increases in heart rate ( HR ) and mean arterial pressure ( MAP ) in response to
TMX (
16.1 - 28.6 m / min ) were similar after icv administration of saline or no treatment . -
pubmed
After icv administration of alpha - helical
CRF9 - 41 , HR and MAP responses during
TMX were significantly attenuated . -
pubmed
In addition ,
TMX - induced elevations of estimated mesenteric vascular resistance and iliac blood flow velocity were blunted after
CRF receptor blockade . -
pubmed
Fasting blood
glucose declined from 12.3 + /- 0.4 to 10.5 + /- 0.7 mmol / L ( p less than 0.05 ) but mean diurnal
glucose and glycosylated
hemoglobin A1c did not change significantly . -
pubmed
The
high - density lipoprotein (
HDL )
cholesterol increased after
weight reduction ( 0.96 + /- 0.06 vs 1.11 + /- 0.05 mmol / L , p = 0.009 ) . -
pubmed
Inhibition of
tumor growth is correlated with
spermidine depletion and an increase of
decarboxylated - S - adenosylmethionine , the
aminopropyl donor in the
spermidine and
spermine synthase reactions . -
pubmed
Finally , this study demonstrates the advantage of using prodrugs of
delta - MFMO , an inhibitor of
ODC , since they possess longer duration of action , higher potency , and in some cases better antitumor efficiency than the parent direct inhibitor of
ODC . -
pubmed
This phase is characterized by increased
FSH and
LH and decreased
progesterone /
estradiol ratio resulting in
irregular bleeding ,
endometrial hyperplasia ,
polyps ,
fibromas ,
mammary dystrophies , disturbance of mood , appetite and thermoregulation . -
pubmed
Lastly , the sensitivity of ovarian follicles to
FSH and
LH is lost with a decline of E2 below 20 pg / ml produced almost exclusively from peripheral conversion from circulating
androgens . -
pubmed
The beneficial effects of
E2 are lost resulting in
atrophy of the sensitive tissues , decreased
calcium absorption , increased
bone resorption , accelerated
bone loss and
osteoporosis , rise in serum
triglycerides , increased
VLDL and
LDL lipoproteins , increased LDL / HDL
cholesterol , a profile which favors
atherosclerosis . -
pubmed
Local and systemic antifungal prophylaxis with oral
amphotericin B plus
itraconazole was given throughout the immediate
post - transplant period and there was no evidence of recurrent
mycosis . -
pubmed
Thermotropic behavior of
dimyristoylphosphatidylcholine in the presence of
cytochrome c oxidase . -
pubmed
Chronic
clonidine treatment produces desensitisation of post- but not presynaptic
alpha 2 - adrenoceptors . -
pubmed
Sympathetic hyperactivity following withdrawal of chronic
clonidine treatment may be mediated by down - regulation of postsynaptic
alpha 2 - adrenoceptors in the CNS . -
pubmed
Stimulation of
insulin secretion by
glucose , the
phorbol ester 12 - O - tetradecanoylphorbol 13 - acetate (
TPA ) or the
sulphonylurea glibenclamide did not affect these
polyamine contents . -
pubmed
In accordance with a role for
protein kinase C in
insulin secretion ,
TPA stimulated both
protein kinase C activity and
insulin secretion . -
pubmed
Spermidine ( 5 mM ) and
spermine ( 1.5 mM ) at concentrations found in islets inhibited
protein kinase C stimulated by
TPA +
phosphatidylserine by 55 % and 45 % respectively . -
pubmed
Inhibition of
protein kinase C by
polyamines showed competition with
Ca2 + , and
Ca2 + influx in response to
glucose or
glibenclamide prevented inhibition of
insulin secretion by exogenous
polyamines at concentrations where they did not affect
glucose oxidation . -
pubmed
It is suggested that inhibition of
protein kinase C by
polyamines may be of significance for regulation of
insulin secretion in vivo and that
Ca2 + influx may function by displacing inhibitory
polyamines bound to
phosphatidylserine in membranes . -
pubmed
Light causes a 4 - -5 - fold decrease in
NAT activity in static organ culture , and inhibits
melatonin release in flow - through organ culture . -
pubmed
Drugs that increase
cyclic nucleotide levels in cells (
cholera toxin ,
RO 20 - 1724 ,
monobutyryl cyclic AMP ,
monobutyryl cyclic GMP ) block the
NAT decrease by light , whereas high
potassium or
EGTA do not block this light - induced
NAT inactivation . -
pubmed
When added to milk
casein and heated ,
histidine appeared to be the most toxic . -
pubmed
Even after treated with
papain , the heated
casein -
histidine mixture was still effective to induce gizzard erosion . -
pubmed
The IC50 were 30 microM and 29 microM for
taurine efflux and 26 and 27 microM for
C1 efflux for NMD and
NTD , respectively . -
pubmed
An analysis of receiver operating characteristic curves revealed that the highest diagnostic accuracies in distinguishing benign from
malignant lung diseases were achieved with TPM ( 81 % ) ,
CYFRA 21 - 1 ( 72 % ) ,
CEA ( 78 % ) or
TPA ( 78 % ) when using cut - off values of 46 Ul-1 , 3.0 micrograms l-1 , 2.0 micrograms l-1 and 75 Ul-1 respectively . -
pubmed
The dorsal , median and pontine raphe nuclei as well as the supralemniscal nucleus ( the
B9 group ) contained very few double - labelled cells , which comprised a mean of 0.1 - 0.7 % of all
serotonin - immunoreactive cells in each of these nuclei . -
pubmed
We suggested the name
Ep - CAM for this
molecule ( Litvinov et al . -
pubmed
The data presented demonstrate that the
Ep - CAM molecules are functionally active in the epithelial and
carcinoma cells tested , are capable of mediating
Ca(++)-independent intercellular adhesions , and are not likely to be involved in cell - substrate adhesion . -
pubmed
We show that
forskolin and
8 - bromo - cAMP block the
basic Fibroblast Growth Factor - induced DNA synthesis , do not inhibit
mitogen activated protein kinase activation whereas they reduce
G1 cyclin ( E and
D1 ) expression without modification of
cyclin A level . -
pubmed
These results suggest that
cAMP may exert its antiproliferative effects through the regulation of
cyclin synthesis and
cyclin - dependent kinase activation . -
pubmed
The expression of the
ATPase gene (
PMA1 ) is regulated by
glucose ( Rao , R. , Drummond - Barbosa , D. , and Slayman , C. W. ( 1993 ) Yeast 9 , 1075 - 1084 ) and by the
TUF /
RAP1 /
GRF1 transcription factor ( Capieaux , E. , Vignais , M.-L. , Sentenac , A. , and Goffeau , A. -
pubmed
Expression of
APA1 gene is regulated by the
carbon source and requires the protein
GCR1 . -
pubmed
Deletion of
APA1 causes a defective regulation of the
PMA1 expression by
glucose but has not noticeable effect on the expression of other
TUF - regulated genes . -
pubmed
Nevertheless the expression of
glucose - repressible
HXT3 and
SNF3 genes is significantly reduced . -
pubmed
These results suggest a model in which
APA1 acts on a
glucose - signaling pathway that controls the expression of several genes that are transcriptionally regulated by
glucose . -
pubmed
The
glutamine synthetase (
GS ) activity of both cell lines was elevated ( up to x 9 ) as
glutamine was depleted from normal media ( GMEM + gmine ) . -
pubmed
However , there was no apparent significant difference between the two cell lines in the pattern of changes of
GS activity in response to
glutamine . -
pubmed
The discrepancy between this value and the molecular mass of 15,456 + /- 1.5 Da for the
copper - free protein determined by electrospray mass spectrometry can be explained by a
post - translational processing of the gene product . -
pubmed
Subsequently , the precursor is cleaved by a
signal peptidase II - like
protease and then acetylated at its NH2 - terminal
alpha - amino group . -
pubmed
A comparison of the primary structure of
halocyanin with a number of other blue
copper proteins places it into the
plastocyanin - related group . -
pubmed
After the heart rate was doubled from 60 to 120 beats / min ,
lactate efflux increased from 0.23 + /- 0.10 (
SE ) to 0.45 + /- 0.12 mumol.min-1.g-1 dry weight with a mean response time of 21.3 s but without an overshoot . -
pubmed
DNA sequencing of the
V beta RT - PCR products reveals a heterogeneous population of VDJ recombinations and deduced
CDR3 amino acid sequences . -
pubmed
Plasma
LH levels were not altered by the injection of
amphetamine . -
pubmed
Gas chromatography (
GC ) of free
amines is generally unsatisfactory owing to the adsorption and decomposition of the solutes on the column . -
pubmed
Derivatization of
amines is employed to reduce the polarity and to improve the
GC properties . -
pubmed
Derivatization reactions for the determination of
amines by
GC are reviewed with respect to reactivity , selectivity and sensitivity . -
pubmed
Blocking of
glioma proliferation in vitro and in vivo and potentiating the effects of
BCNU and
cisplatin :
UCN-01 , a selective
protein kinase C inhibitor . -
pubmed
Seven - hydroxystaurosporine (
UCN-01 ) is a derivative of the nonselective
protein kinase inhibitor
staurosporine that exhibits significant selectivity for
protein kinase C (
PKC ) in comparison to a variety of other intracellular
kinases and appears to be well tolerated in vivo at concentrations sufficient to achieve effective inhibition of
PKC . -
pubmed
The base substitution observed in the BstNI site of Bio -
Burs and
Bursine-2 was responsible for changing the
amino acid at position 222 to
serine . -
pubmed
The
amino acid at this position has been reported to influence a neutralizing epitope on
VP2 . -
pubmed
Upregulated protein
spots were cut from multiple 2 - D gels collected and run on a modified funnel gel to concentrate the
proteins and remove the
sodium dodecyl sulfate before digestion . -
pubmed
A digest can be reanalyzed after
deuterium exchange to obtain a second , orthogonal data set to increase the confidence level of
protein identification . -
pubmed
Kinetics of
calcium release from
manganese peroxidase during thermal inactivation . -
pubmed
In this work we determined that four
Ca2 + ions are present in the
enzyme as isolated but this was reduced to 2 mol / mol upon treatment with
Ca2 + -chelating agents or extensive dialysis of dilute
enzyme . -
pubmed
One of two relatively tightly bound
Ca2 + remaining in the
enzyme was released during thermal inactivation at pH 7.2 . -
pubmed
Inactive
enzyme contained one
Ca2 + which could be removed in
acidic conditions . -
pubmed
The weakly associated
Ca2 + normally present in the
enzyme did not affect
enzyme activity and did not seem to protect the
enzyme from thermal inactivation at submicromolar
enzyme concentrations . -
pubmed
Excess
Ca2 + or
Mn2 + decreased the rate of the thermal inactivation and
Mn2 + stabilized the
enzyme more efficiently than
Ca2 + at higher temperature . -
pubmed
Enzyme stabilization by
Mn2 + was proposed to be due to binding of
Mn2 + to the
Mn2 + substrate binding site . -
pubmed
Moreover ,
Ca2 + was a poor inhibitor of
manganese peroxidase activity at pH 4.5 . -
pubmed
The present studies examined the distribution and density of the
mu and delta receptors in specific brain regions that may mediate the rewarding and reinforcing effects of
ethanol , using quantitative autoradiography and the specific mu agonist
FK 33 - 824 and delta agonist
DPDPE , in their iodinated form . -
pubmed
GTP cyclohydrolase I (
GTPCH ) is the rate - limiting enzyme in the formation of
tetrahydrobiopterin , the cofactor for
catecholamine ,
indolamine and
nitric oxide biosynthesis . -
pubmed
DESIGN : The nocturnal ( 2230 h-0200 h )
serum TSH surge , the
TSH response to
TRH ( 200 microgram as an i.v . -
pubmed
Serum TSH levels were measured in samples taken just before ( time 0 ) and 30 minutes , after
TRH administration . -
pubmed
A role for
NO as a mediator of
N - formyl - methionyl - leucyl - phenylalanine (
fMLP)-induced chemotaxis was supported by the finding that the
NO synthase (
NOS ) inhibitor
L - NMMA ( 500 microM ) inhibited chemotaxis ; EC50 for fMLP 28.76 + /- 5.62 and 41.13 + /- 4.77 pmol/10(6 ) cells with and without
L - NMMA , respectively . -
pubmed
A role for
cyclic GMP as a mediator of chemotaxis was supported by the finding that the
guanylyl cyclase inhibitor
LY 83583 ( 100 microM ) completely inhibited chemotaxis and suppressed the maximal response ; EC50 for fMLP 32.53 + /- 11.18 and 85.21 + /- 15.14 pmol/10(6 ) cells with and without
LY 83583 , respectively . -
pubmed
The same pattern of inhibition was observed with the
G - kinase inhibitor
KT 5823 ( 10 microM ) ; EC50 for fMLP 32.16 + /- 11.35 and & gt ; 135 pmol/10(6 ) cells with and without
KT 5823 , respectively . -
pubmed
The
phosphatase inhibitor ,
2,3 - diphosphoglyceric acid (
DPG ) ( 100 microM ) which inhibits
phospholipase D , attenuated fMLP - induced chemotaxis ; EC50 for fMLP 19.15 + /- 4.36 and 61.52 + /-
16.2 pmol/10(6 ) cells with and without
DPG , respectively . -
pubmed
Although the
NOS inhibitors
L - NMMA and
L - canavanine ( 500 microM ) failed to inhibit fMLP - induced
SAG ,
carboxy - PTIO caused significant inhibition ( EC50 for fMLP 36.15 + /- 7.43 and 86.31 + /- 14.06 nM and reduced the maximal response from 22.14 + /- 1.5 to 9.8 + /- 1.6 nmol
O2-/10(6 ) cells/10 min with and without
carboxy - PTIO , respectively ) . -
pubmed
This suggests
NO is a mediator of fMLP - induced
SAG . -
pubmed
A role for
cyclic GMP as a mediator of
SAG was supported by the effects of
G - kinase inhibitors
KT 5823 ( 10 microM ) and
Rp-8 - pCPT - cGMPS ( 100 microM ) which inhibited
SAG giving EC50 for fMLP of 36.26 + /- 8.77 and 200.01 + /- 43.26 nM with and without
KT 5823 , and 28.35 + /- 10.8 and 49.25 + /- 16.79 nM with and without
Rp-8 - pCTP - cGMPS . -
pubmed
The
phosphatase inhibitor
DPG ( 500 microM ) inhibited
SAG ; EC50 for fMLP 33.93 + /- 4.23 and 61.12 + /- 14.43 nM with and without
DPG , respectively . -
pubmed
In conclusion , chemotaxis and
SAG induced by fMLP can be attenuated by inhibitors of
phospholipase D ,
NO and
cyclic GMP , suggesting a role for these agents in neutrophil activation . -
pubmed
The
HDL cholesterol concentrations and the ratios of
HDL cholesterol to total
cholesterol were significantly decreased in the subjects with
chronic infection ( 1.24 versus 1.36 mmol / L , P = .026 ; and 0.19 versus 0.22 , P = .018 , respectively ) . -
pubmed
In animals , NSAIDs inhibit the
cyclooxygenase (
COX ) activity of
prostaglandin endoperoxide H synthase , which ultimately blocks the formation of
prostaglandins . -
pubmed
In this study we show that NSAIDs act as competitive inhibitors of
allene oxide synthase (
AOS ) , the
cytochrome P450 that initiates plant
oxylipin synthesis . -
pubmed
We also show that
aspirin causes the time - dependent inhibition and acetylation of
AOS , which leads the irreversible inactivation of this
enzyme . -
pubmed
It is possible that
aspirin and related NSAIDs could inhibit other
P450s that have motifs similar to
AOS and consequently serve as potential biochemical targets for this class of drugs . -
pubmed
The first database was obtained from eight endurance - trained men dehydrated to four different levels ( 1.1 , 2.3 , 3.4 , and 4.2 % of body wt ) during 120 min of cycling at a power output of 62 - 67 % maximum
O2 consumption ( VO2 max ) in the heat [ 33 degrees C and 50 % relative humidity (
RH ) ] . -
pubmed
Secretin and other hormones raising intracellular
cyclic adenosine monophosphate (
cAMP ) concentrations promote biliary
HCO3 secretion by stimulating apical
Cl- channels and
Cl-/
HCO3- exchange (
AE2 ) . -
pubmed
Suppression by
anti - CD44v6 apparently functioned distinctly to
cyclosporine and was most effective in combination with presensitization . -
pubmed
Selenium concentrations in blood , milk , casein , and liver and
glutathione peroxidase activity in blood and liver are reported as responses per milligram of
Se intake . -
pubmed
Although
Linomide has unacceptable side effects , it seems to have a modest effect on
MS disease activity , as measured by MRI . -
pubmed
This
hepatomegaly can not be explained solely by lipid accumulation and is accompanied by significant increases in hepatocyte proliferative activity ( as evidenced by increased
Erk activation , cell - cycle related gene expression ,
bromodeoxyuridine incorporation , and hepatic DNA content ) with concomitant inhibition of hepatocyte apoptosis ( as evidenced by decreased numbers of apoptotic hepatocytes , induction of several antiapoptotic mechanisms , and decreased activation of
procaspase 3 ) . -
pubmed
BACKGROUND : One mechanism that directs the action of the second messengers ,
cAMP and
diacylglycerol , is the compartmentalization of
protein kinase A (
PKA ) and
protein kinase C (
PKC ) . -
pubmed
Phenylalanine-24 in the N - terminal region of
ammodytoxins is important for both enzymic activity and presynaptic
toxicity . -
pubmed
To address this problem , a surface - exposed aromatic residue ,
Phe(24 ) , in the N - terminal region of the most potent
Atx ,
AtxA , was replaced by other aromatic (
tyrosine ,
tryptophan ) , hydrophobic (
alanine ) and polar uncharged (
serine ,
asparagine ) residues . -
pubmed
Our results suggest a critical involvement of
Phe(24 ) in the
neurotoxicity of
AtxA , apparently at a stage which does not involve the interaction with the known
Atx - binding neuronal proteins and catalytic activity . -
pubmed
GABA has been reported to stimulate feeding via both ionotropic
GABA(A ) and
metabotropic GABA ( B ) receptors . -
pubmed
Within the heterodimer , the
GABA - binding site is localized to
GABA ( B ) R1 . -
pubmed
In the ventrolateral part of the arcuate nucleus ,
GABA ( B ) R1 - immunoreactive cell bodies were shown to contain pro - opiomelanocortin and
cocaine- and
amphetamine - regulated transcript . -
pubmed
In the tuberomammillary nucleus ,
GABA ( B ) R1 - immunoreactive cell bodies expressed
histidine decarboxylase , a marker for
histamine - containing neurones . -
pubmed
The results may provide a morphological basis for the understanding of how
GABA regulates the hypothalamic control of food intake and
body weight via
GABA ( B ) receptors . -
pubmed
Gasless VTEM involves a modification of transanal endoscopic microsurgery (
TEM ) that incorporates a standard laparoscopic video camera without a
CO ( 2 ) insufflation system . -
pubmed
The
strain GR4 , which was less competitive than strain
S24 , was mutagenized with
N - methyl - N '- nitro - N - nitrosoguanidine to obtain pigment - diverse mutants and six selected mutants were screened for symbiotic parameters . -
pubmed
Since the drug regimens were different , summed
PI for the first
acetaminophen dose interval ( SUMPI3.5 - 6 ) and the first
naproxen dose interval ( SUMPI3.5 - 9 ) was calculated . -
pubmed
In addition , a number of genes were identified which predicted integral membrane proteins with extracellular
C - type lectin or
immunoglobulin domains and intracellular immunoreceptor
tyrosine - based inhibitory motifs ( ITIMs ) and immunoreceptor
tyrosine - based activation motifs ( ITAMs ) ( plus their associated signal transduction molecules ) , suggesting that activating and inhibitory receptors have an
MHC - independent function and an early evolutionary origin . -
pubmed
Furthermore the most potent compounds 2 g and 3 g from each series were screened in vitro for inhibition of both
COX-2 and
COX-1 catalysed
prostaglandin biosynthesis ( radiochemical assay ) . -
pubmed
Like most of the commercially available non - steroidal anti - inflammatory drugs ( NSAIDs ) , in the in vitro study , compounds 2 g and 3 g showed anti - inflammatory activity by blocking the metabolism of
arachidonic acid to
prostaglandin via the
cyclooxygenase pathways . -
pubmed
Subunit - dependent effects of
nickel on
NMDA receptor channels . -
pubmed
Application of
Ni2 + showed that stimulation of
NR1a -
NR2B receptor channels was dependent on external pH , while voltage - independent inhibition of
NR1a -
NR2A was less sensitive to pH change . -
pubmed
These results indicate that
Ni2 + has multiple and complex effects on
NMDA channels , which are largely dependent on the
NR2 subunit . -
pubmed
farciminosum isolates were evaluated for
urease activity on Christensen 's
urea agar slants and for other qualitative and quantitative
urease assays of activity . -
pubmed
2.9)% ,
cholesterol 4.8 ( 0.8)mmol / l ,
triglycerides 2.7 ( 1.6)mmol / l ,
HDL 0.83 ( 0.2)mmol / l ; 60 % had
albuminuria ( micro 38 % , macro 22 % ) , 47 % were
hypertensive , 7 % ( n = 2 ) had
retinopathy , 24 % had
peripheral neuropathy , none had
peripheral vascular disease , 14 % had documented coronary vascular and one participant
cerebrovascular disease . -
pubmed
superoxide dismutase and
catalase activity , mucus and total gastric tissue
sulfhydryl group were increased . -
pubmed
It is interesting to note that the metal poison , coordinating anions
cyanide and
azide are also rather weak
Cab inhibitors ( Ki in the range of 27 - 55 mM ) , whereas
sulfamide is a very weak
Cab inhibitor ( Ki of 103 mM ) , although it strongly inhibits Cam ( Ki of 70 microM ) . -
pubmed
Surprisingly ,
phenylboronic and phenylarsonic acids , which have been investigated for the inhibition of all these
CAs for the first time , showed very weak activity against the
alpha - CA isozymes , but were effective
Cab and
Cam inhibitors . -
pubmed
The best
Cab inhibitors were just these two compounds ( Ki 's of 0.20 - 0.33 mM ) , whereas the best
Cam inhibitor was
sulfamic acid ( Ki of 96 nM ) . -
pubmed
These major differences of behavior between the diverse
CAs investigated here toward anion inhibitors can be difficultly explained considering the convergent evolution of so diverse enzymes for the binding and turnover of small molecules such as
carbon dioxide and anions . -
pubmed
RESULTS : Pathologists , by detecting blue dye or
carbon particles or alterations in
nodal cell populations should attempt to confirm that a node submitted as sentinel is truly sentinel . -
pubmed
Ryanodine receptors are found especially within the cerebellum , and alterations in
calcium signaling within the cerebellum could impair long - term
depression and subsequent motor learning . -
pubmed
Biochemical characterization in vitro showed that both
ACPs can be loaded with a
malonate extender unit by the
LnmG acyl transferase ; however , ACP(6 - 2 ) appears to be preferred because the loading efficiency is about 5 - fold that of ACP(6 - 1 ) . -
pubmed
These findings provide new insights into the
polyketide chain skipping mechanism for modular
PKSs . -
pubmed
EPR spectroscopy of
Cu ( I )- NO adsorption complexes formed over
Cu -
ZSM-5 and
Cu -
MCM-22 zeolites . -
pubmed
The
Cu ( I )- NO adsorption complexes were formed over
copper exchanged and autoreduced high siliceous
Cu -
ZSM-5 and
Cu - MCM-22 zeolites and studied by
EPR spectroscopy at X- , Q- , and W - band frequencies . -
pubmed
The
EPR signal intensity of the
Cu ( I )- NO species was studied as a function of the
NO loading , and the formation of diamagnetic
Cu ( I )-( NO )( 2 ) species with rising
NO pressure at the expense of paramagnetic
Cu ( I )- NO monomers could be demonstrated for both systems at low temperatures . -
pubmed
In the present study , we attempted to find out whether metabolic and physiologic characteristics of fibres are important in determining skeletal muscle response to
clenbuterol ( an
adrenergic receptor agonist ) therapy , as proposed in the treatment of
wasting disorders . -
pubmed
A significant decline in the
succinic dehydrogenase activity was observed , thereby suggesting abnormality in oxidative activity of skeletal muscles in response to
clenbuterol therapy . -
pubmed
The highest
amylase production were attained with
lactose ,
maltose ,
xylose and starch as
carbon sources . -
pubmed
Other
carbohydrates supported growth but did not induce
amylase synthesis and
glucose repressed it , indicating catabolite repression in this microorganism . -
pubmed
Of the
nitrogen sources tested ,
casaminoacids ,
ammonium nitrate and
sodium nitrate stimulated the highest yield of
amylase . -
pubmed
No study has evaluated the association of
NHL with postload plasma
glucose (
PLG ) levels , which are positively associated with
BMI . -
pubmed
The study compared the net decline of intramyocellular lipids ( IMCL ) during exercise ( n = 18 ) measured by biochemical assay ( BIO ) and
Oil Red O ( ORO ) staining on biopsy samples from vastus lateralis muscle and by
( 1 ) H - MR spectroscopy (
MRS ) sampled in an 11 x 11 x 18 - mm(3 ) voxel in the same muscle . -
pubmed
Group I , control , was injected intraperitoneally ( ip ) with 1 ml of saline solution ; group
II , received DL50 of PQ , ip ; groups III and
IV , DL50 of PQ , and simultaneously the first dose of
MLT ( 15 mg / kg , ip ) or
STS ( 1,5 g / kg , i.p . ) -
pubmed
Thirty minutes later , groups III and
IV received a second similar dose of
MLT and TSS . -
pubmed
METHODS :
Ala ( GTT ) or
Val ( GCT ) polymorphism in the signal peptide of
Mn - SOD gene was evaluated using a primer pair to amplify a 107 - bp fragment followed by digestion with restriction enzyme NgoM
IV . -
pubmed
Intrathecal administration of
NAGly ( 700 nmol ) and the
pan - cannabinoid receptor agonist
HU-210 ( 30 nmol ) reduced the mechanical
allodynia induced by partial ligation of the sciatic nerve . -
pubmed
The
NAGly induced anti -
allodynia was dose dependent and , unlike
HU-210 , was unaffected by the cannabinoid CB(1 ) and
CB ( 2 ) receptor antagonists ,
AM251 and
SR144528 ( 30 nmol ) . -
pubmed
NTS1 monomers activate
G alpha q beta ( 1 ) gamma ( 2 ) , whereas receptor dimers catalyze
nucleotide exchange with lower affinity . -
pubmed
CONTEXT : Evidence suggests that
testosterone ( T ) influences
insulin sensitivity in men . -
pubmed
MAIN OUTCOME MEASURES : Each evaluation comprised a 75 - g oral
glucose tolerance test with assessment of
insulin sensitivity ( fasting
insulin levels , homeostatic model assessment for
insulin resistance , and Matsuda
insulin sensitivity index ) and
insulin secretion ( corrected
insulin response ) . -
pubmed
Serum
cortisol ,
leptin ,
adiponectin , free
fatty acids ,
IL-6 ,
C - reactive protein , and
TNF - alpha levels were also measured . -
pubmed
CONCLUSIONS : 1 ) Acute sex
steroid withdrawal reduces
insulin sensitivity in young healthy
IHH men . -
pubmed
2 ) The acuity of the
hypogonadism and absence of changes in
body mass index or
leptin levels suggest that sex
steroids modulate
insulin sensitivity in the absence of apparent or detectable changes in body composition . -
pubmed
WT1 silencing significantly reduced the expression of
Nestin and
Zyxin and resulted in inhibition of
melanoma cell proliferation as determined by a reduced
BrdU incorporation . -
pubmed
Neurosteroid modulation of
GABAA receptors : molecular determinants and significance in health and disease . -
pubmed
Identified molecular targets underlying modulation of brain excitability include both the inhibitory
GABA(A ) and the excitatory
NMDA receptor . -
pubmed
A range of molecular mechanisms including the subunit composition of the receptor(s ) , phosphorylation and local
steroid metabolism , underpin the region- and neuronal selectivity of action of
neurosteroids at synaptic and extrasynaptic
GABA ( A ) receptors . -
pubmed
GABA - based therapeutic approaches :
GABA ( A ) receptor subtype functions . -
pubmed
Here , we will review recent progress in identifying the
neurosteroid binding site on the
GABA ( A ) receptor , and discuss how these discoveries will impact on our understanding of the role of
neurosteroids in health and disease . -
pubmed
Moreover , when NEC lesions were restricted to the colon , small intestinal tissues that seemed clinically healthy had decreased in vitro
glucose absorption due to reduced uptake via the
sodium - dependent glucose transporter with little or no involvement of the apical facilitative
glucose carrier . -
pubmed
Conversely , the hormone
gibberellin (
GA ) can antagonise the effects of
KNOX overexpression and reduce leaflet number , suggesting that
GA may be a negative regulator of leaflet formation . -
pubmed
The aim of this paper was to measure the changing desorbable fraction and bioaccessibility of
phenanthrene in two different soils with increasing soil -
phenanthrene contact time using supercritical fluid extractions (
SFE ) . -
pubmed
Desorption profiles were measured every 7d using selective
SFE conditions and the results were compared to
14C - phenanthrene mineralisation assays . -
pubmed
2H2O (
sodium pentacyanonitrosylferrate ( II ) ,
nitroprusside ,
SNP ) and
MeN ( H ) OH (
N - methylhydroxylamine ,
MeHA ) has been studied by means of UV - vis spectroscopy , using complementary solution techniques : FTIR /
ATR ,
EPR , mass spectrometry and isotopic labeling (
15NO ) , in the pH range 7.1 - 9.3 , I=1 M (
NaCl ) . -
pubmed
In the C - terminal
reductase domain , the complex structure with the substrate at 2.56 - A resolution unexpectedly showed a
ribitylimino intermediate bound at the active site , and hence suggested that the
ribosyl reduction occurs through a
Schiff base pathway . -
pubmed
Lys151 seems to have evolved to ensure specific recognition of the
deaminase product rather than the substrate . -
pubmed
A detailed comparison reveals that the
reductase and the pharmaceutically important enzyme ,
dihydrofolate reductase involved in the
riboflavin and
folate biosyntheses , share strong conservation of the core structure , cofactor binding , catalytic mechanism , even the substrate binding architecture . -
pubmed
Two stronger
EGFR ligands ,
Spitz and
Keren , are expressed by the AMPs themselves and provide an additional , autocrine
mitogenic stimulus to the AMPs during late larval stages . -
pubmed
Nucleoside diphosphate kinase (
NDPK ) (
EC 2.7.4.6 ) , the enzyme transferring the
phosphate residue from
ATP to
nucleoside diphosphates , is localized mainly in the cytoplasm and mitochondria and in smaller amounts in cell nuclei and the microsomal fraction . -
pubmed
Both
phytochrome A - activated
NDPKs purified to electrophoretic homogeneity have the same molecular mass ( 17 - 18 kDa ) determined by
SDS / PAGE . -
pubmed
These data suggest that
Gpx4 differentially regulates apoptogenic protein release owing to its inner membrane location in mitochondria and its ability to repair
cardiolipin peroxidation . -
pubmed
Single
nucleotide polymorphism ( SNP ) sites for
IFN - gamma ( +874 T / A ) ,
IL-10 ( -1,082 G / A , -819 C / T , -592 C / A ) ,
TNF - alpha ( -308 G / A ) , and
TGF - beta1 ( codon 10 T / C , codon 25 G / C ) genes were observed with the TaqMan allelic discrimination assay . -
pubmed
Hourly blood sampling from 8 h before and until 24 h after the birth of the first piglet was used for investigation of temporal changes in plasma concentrations of
oxytocin ,
cortisol , and
ACTH . -
pubmed
There was a clear temporal development in sow behavior and hormone concentrations (
ACTH ,
cortisol , and
oxytocin ) across parturition ( P & lt ; 0.001 ) , independent of treatment . -
pubmed
Floor heating increased the mean concentration of
cortisol ( P = 0.02 ; estimated as 29 % greater than in controls ) and tended to increase the mean concentration of
ACTH ( P = 0.08 ; estimated as 17 % greater than in controls ) , but we did not find any treatment effect on mean
oxytocin concentrations , the course of parturition , or the behavior of sows . -
pubmed
In addition , exposure to heat decreased the between - sow variation of plasma
oxytocin ( approximately 31 % less relative to control ) and
ACTH ( approximately 46 % less relative to control ) . -
pubmed
Ventilation in response to exercise was increased in PG ( 49.4+/-6.6L / min ) compared to
NP ( 39.8+/-5.4 L / min , at 100W ; p<0.05 ) women , and was further augmented by
obesity ( 56.7+/-9.3 L / min , at 100W ; p<0.05 versus PG ) secondary to an elevated metabolic cost of exercise as indicated by no further increase in .V(E)/.V(
CO2 ) and .V(E)/.V(
O2 ) in PGOB compared to PG women . -
pubmed
Docking studies support that 2 binds to the
ATP - noncompetitive pocket of
glycogen synthesis kinase-3beta (
GSK-3beta ) , which is a putative target of
manzamines . -
pubmed
It indicates that
IL11 , along with its specific receptor ,
IL11R alpha , and downstream
signalling molecules ,
STAT3 and
SOCS3 , are likely to play a role in the progression of
endometrial carcinoma . -
pubmed
We determined the effects of
vitamin D deficiency on bone metabolism :
calcium ,
phosphate and
PTH levels . -
pubmed
We also analyzed biochemical markers of bone remodeling and bone mineral density (
BMD ) before and 6 months after
vitamin D replacement . -
pubmed
Angiotensin - converting enzyme 2 (
ACE2 ) catabolizes
angiotensin II (
Ang II ) to
angiotensin 1 - 7 (
Ang -( 1 - 7 ) ) to limit effects of the
renin -
angiotensin system . -
pubmed
OBJECTIVES : We conducted a prospective randomized trial to compare the clinical impact of conventional risk factor modification to that associated with the addition of coronary artery
calcium (
CAC ) scanning . -
pubmed
RESULTS : Compared with the no - scan group , the scan group showed a net favorable change in
systolic blood pressure ( p = 0.02 ) ,
low - density lipoprotein cholesterol ( p = 0.04 ) , and waist circumference for those with increased abdominal girth ( p = 0.01 ) , and tendency to
weight loss among
overweight subjects ( p = 0.07 ) . -
pubmed
Within the scan group , increasing baseline
CAC score was associated with a dose - response improvement in systolic and diastolic blood pressure ( p & lt ; 0.001 ) , total
cholesterol ( p & lt ; 0.001 ) ,
low - density lipoprotein cholesterol ( p & lt ; 0.001 ) ,
triglycerides ( p & lt ; 0.001 ) , weight ( p & lt ; 0.001 ) , and Framingham Risk Score ( p = 0.003 ) . -
pubmed
TEM analysis and X - ray diffraction ( XRD ) results indicate that the
Bi2O3 - core /
SnO2 - shell nanowires consist of pure tetragonal alpha -
Bi2O3 - phase momocrystalline cores and tetragonal
SnO2 - phase polycrystalline shells . -
pubmed
Glutamate dehydrogenase (
GDH ) is a mitochondrial enzyme linking the Krebs cycle to the multifunctional
amino acid glutamate . -
pubmed
Thereby ,
GDH plays a pivotal role between
carbohydrate and protein metabolisms , controlling production and consumption of the messenger molecule
glutamate in neuroendocrine cells . -
pubmed
Indeed ,
GDH directly depends on the provision of the co - factor
NADH /
NAD (+ ) , rendering the enzyme sensitive to the redox status of the cell . -
pubmed
Moreover ,
GDH is allosterically regulated by
GTP and
ADP . -
pubmed
GDH is also regulated by
ADP - ribosylation , mediated by a member of the energy - sensor family sirtuins , namely
SIRT4 . -
pubmed
In the brain ,
GDH ensures the cycling of the neurotransmitter
glutamate between neurons and astrocytes . -
pubmed
GDH also controls
ammonia metabolism and detoxification , mainly in the liver and kidney . -
pubmed
We found that exposure to
oxaliplatin induced a significant increase in
LC3 lipidation and subsequent
LC3 puncta formation . -
pubmed
While the proliferation of
HCC cells was inhibited upon
oxaliplatin exposure , inhibition of autophagy by
ATG7 interference and
chloroquine pre - treatment further increased the sensitivity to chemotherapy . -
pubmed
All samples were characterized by SEM ,
AFM and thermogravimetric analysis , and the mechanical properties of the
PAA grafted sample were determined . -
pubmed
At a concentration of 100 muM , 42 natural products inhibited
OATP1B1 - mediated
[ ( 3 ) H ] E3S uptake by more than 50 % , and five of them significantly inhibited
OATP1B1 - mediated
[ ( 3 ) H ] E3S by more than 80 % with the following rank order of potency :
quercetin & gt ;
astragaloside IV & gt ;
icariin & gt ;
glycyrrhizic acid & gt ;
ginsenoside Rc . -
pubmed
We performed
sevoflurane anaesthesia induction , the end - tidal was kept at 3 and 4 % , during 15 min at each concentration , with no analgesic drug and no neuromuscular blocking agent , and we applied a nociceptive stimulus :
tetanus 100 Hz , during 5 s. We set the standard monitorization , BIS , RE , and state entropy (
SE ) along the study . -
pubmed
There was a significant difference between RE and
SE post - noxious stimulus values at 3 and 4 % end - tidal
sevoflurane ( p & lt ; 0.05 ) . -
pubmed
The hypothesis that
letrozole would arrest growth of the dominant follicle , resulting in emergence of a new follicular wave at a predictable
post - treatment interval , was tested . -
pubmed
Plasma concentrations of
LH and
FSH were measured by radioimmunoassay ; plasma concentrations of
letrozole were determined by high - performance liquid chromatography tandem mass spectrometry . -
pubmed
A single intravenous dose of
letrozole did not induce regression of the dominant follicle present at the time of treatment , nor did it directly affect
FSH release . -
pubmed
Conversely , treatment with
letrozole increased endogenous concentrations of
LH and extended the lifespan of the dominant follicle , which delayed the next
FSH surge and subsequent follicular wave emergence . -
pubmed
The proposed biosynthesis of
mirubactin comprises the iterative use of a stand - alone carrier - protein - bound substrate , as well as an
ester - bond - forming step catalyzed by a C - terminal condensation domain , thus revealing an interesting system for further biochemical studies to gain a deeper understanding of
nonribosomal peptide synthetase - catalyzed
siderophore biosynthesis . -
pubmed
Molecular mechanisms involved in the synergistic interaction of the
EZH2 inhibitor
3 - deazaneplanocin A with
gemcitabine in
pancreatic cancer cells . -
pubmed
Pancreatic ductal adenocarcinoma (
PDAC ) is characterized by overexpression of
enhancer of Zeste homolog-2 (
EZH2 ) , which plays a pivotal role in
cancer stem cell ( CSC ) self - renewal through methylation of
histone H3 lysine-27 ( H3K27me3 ) . -
pubmed
Against this background ,
EZH2 was identified as an attractive target , and we investigated the interaction of the
EZH2 inhibitor
DZNeP with
gemcitabine . -
pubmed
Moreover ,
DZNeP enhanced the mRNA and protein expression of the
nucleoside transporters
hENT1 /
hCNT1 , possibly because of the significant reduction of
deoxynucleotide content ( e.g. -
pubmed
Furthermore ,
DZNeP and
DZNeP /
gemcitabine combination significantly reduced the volume of
PDAC spheroids growing in CSC - selective medium and decreased the proportion of
CD133 + cells . -
pubmed
The role of
fluorine in stabilizing the bioactive conformation of
dihydroorotate dehydrogenase inhibitors . -
pubmed
Dihydroorotate dehydrogenase (
DHODH ) is an important drug target due to its prominent role in
pyrimidine biosynthesis . -
pubmed
Leflunomide and
brequinar are two well - known
DHODH inhibitors , which bind to the enzyme in the same pocket with different binding modes . -
pubmed
We have recently realized a series of new inhibitors based on the
4 - hydroxy-1,2,5 - oxadiazole ring , whose activity profile was found to be closely dependent on the degree of
fluorine substitution at the
phenyl ring adjacent to the
oxadiazole moiety ; a positive influence of
fluorine on the
DHODH inhibitory potency was observed previously [ Baumgartner et al . -
pubmed
For
pilin antigenic variation ( Av ) to occur , a
guanine quartet (
G4 ) structure must form upstream of
pilE. -
pubmed
Different studies4 , 5 relate that stress increases in children and adolescents with
overweight and
obesity , and consequently their salivary physiological responses ( AEA salivary
alpha - amylase ,
cortisol ,
cytokines ,
leptin ) , so in this study we relate these two parameters to see their progress through a program of physical activity . -
pubmed
These results show that
PVA -
SNO films might emerge as a new material with potential for the topical treatment of
microvascular skin disorders . -
pubmed
In vivo efficacy of
HD0471953 : a novel
GPR119 agonist for the treatment of
type 2 diabetes mellitus . -
pubmed
We examined the relationship between childhood measures of
PAH exposure and prevalence of
attention deficit / hyperactivity disorder (
ADHD ) ,
learning disability (
LD ) , and special education (
SE ) in a nationally representative sample of 1,257 U.S. children 6 - 15 years of age . -
pubmed
Multivariate logistic regression accounting for survey sampling was used to determine the associations between
PAH metabolites and
ADHD ,
LD , and
SE . -
pubmed
Finally , for a more radical approach towards a safe
MEK inhibitor , we validate the potential of multi - kinase inhibitor
curcumin , especially the nano -
curcumin made out of pure
curcumin with greater bioavailability ; in repealing tumour - shed
TGF - beta - induced Treg cell augmentation . -
pubmed
Decrease in
ubiquitin -
HIF-1alpha was also shown , and leads to higher
HIF-1alpha protein levels in
DZN group . -
pubmed
We showed that
LOX3 activity and its ability to produce
9 - hydroperoxyoctadecadienoic acid (
9 - HPOD ) from
linoleic acid were significantly lower in transgenic seeds than in wild - type seeds by measuring the ultraviolet absorption of
9 - HPOD at 234 nm and by high - performance liquid chromatography . -
pubmed
In a
post - hoc analysis , non - depressed non - obese women were found to have significantly higher
cortisol levels compared to women with
depression ,
obesity or both ( p & lt ; 0.05 ) . -
pubmed
The major cellular
fatty acids were
C18 : 1omega7c /
C18 :
1omega6c , C16 : 0 and C16 : 1omega7c / C16 :
1omega6c . -
pubmed
H2S is synthesized from
L - cysteine and/or
L - homocysteine by
cystathionine beta - synthase ,
cystathionine gamma - lyase and
cysteine aminotransferase together with
3 - mercaptopyruvate sulfurtransferase . -
pubmed
H2S is enzymatically oxidized in mitochondria to
thiosulfate and
sulfate by specific enzymes ,
sulfide :
quinone oxidoreductase ,
persulfide dioxygenase ,
rhodanese and
sulfite oxidase . -
pubmed
To explore the role of the canonical
BMP4 /
SMAD signaling pathway in the pathogenesis of
chordoma , we investigated , in 40
skull base chordomas , the expression of three major components of the signaling axis :
BMP4 ,
phospho -
SMAD5 and
SMAD4 . -
pubmed
BACKGROUND :
Acute kidney injury due to
glomerular bleeding has been described with
IgA nephropathy and supratherapeutic
warfarin anticoagulation . -
pubmed
When exposed to low intensities of light that modify the redox state of the
plastoquinone pool , the so - called state transitions redistribute energy between
photosystem I and II . -
pubmed
The
phospholipid cardiolipin is indispensable for eukaryotes to activate mitochondria , and it was previously reported that two phylogenetically distinct types of enzyme synthesizing
cardiolipin , one with two
phospholipase D domains (
CLS_
pld ) and the other with a
CDP - alcohol phosphatidyltransferase domain (
CLS _ cap ) , are patchily and complementarily distributed at higher taxonomic ( e.g. -
pubmed
albolabris venom elicited strong antibody responses , and from their egg yolk
IgY was isolated and purified , which exhibited a single protein band on
SDS - PAGE and two bands ( about 65 and 35 kDa , respectively ) under reduced conditions . -
pubmed
A total of 11 transformation products (
TP ) ( mainly phase - I ) were quantified by liquid chromatography - tandem mass spectrometry ( LC -
MS /
MS ) and their total amount corresponded to 33 % of the
CBZ taken up . -
pubmed
Further 10 TPs ( phase - I and
II ) were identified by LC - high resolution mass spectrometry screening , likely comprising another 12 % of
CBZ . -
pubmed
Effect of Conjugated
Linoleic Acid Supplementation on Serum
Leptin Concentration : A Systematic Review and Meta - Analysis . -
pubmed
BACKGROUND : There are controversies regarding the effect of conjugated
linoleic acid (
CLA ) on serum
leptin . -
pubmed
METHODS : Eleven 2 - piece
ZrO2 implants , each mounted with
ZrO2 abutments plus bonded monolithic
lithium disilicate (
LS2 ) restorations , were grouped for 3.3 mm ( A ) and 4.1 mm ( B ) diameter samples . -
pubmed
CONCLUSIONS : The examined 2 - piece
ZrO2 implant system mounted to
LS2 - restorations seems to be a stable unit under in - vitro conditions with mechanical properties compared to loading capacity of physiological force . -
pubmed
A method for the quantitative determination of
dibutyl phthalate (
DBP ) ,
benzyl butyl phthalate (
BBP ) ,
bis ( 2 - ethylhexyl ) adipate (
DEHA ) ,
bis ( 2 - ethylhexyl ) phthalate (
DEHP ) ,
di - n - octyl phthalate (
DNOP ) ,
dioctyl terephthalate (
DOTP ) ,
diisononyl phthalate (
DINP ) , and
diisodecyl phthalate (
DIDP ) in medical infusion sets was developed and validated using gas chromatography coupled with triple quadrupole mass spectrometry (
GC -
MS /
MS ) in the multiple reaction monitoring ( MRM ) mode . -
pubmed
Chemically , strengthening the previous hypothesis , the collected particles were mainly carbonaceous ( 68%-93 % ) with the maximum budget of the
polyaromatic hydrocarbons being recorded near the surface ( 1st/5th floor : 1.84 , 1.07 , 1.15 for PM10 , PM2.5 and
PM1 , respectively ) . -
pubmed
With respect to the health hazards , the calculation of the
Benzo [ a ] Pyrene toxicity equivalency factors underlined the importance of the height of residence in buildings for the level of the exposure ( 1st/5th floor : B[a]PTEQ : 1.82 , 1.12 , 1.10 , B[a]PMEQ : 1.85 , 1.13 , 1.09 for PM10 , PM2.5 and
PM1 , respectively ) . -
pubmed
Twenty - seven unique UAA alleles identified from the
MHC I genes of these breeds were analyzed concerning
amino acid composition , homology , and phylogenetic relationships . -
pubmed
By systematic mutational analysis , we define conserved dual bi -
arginine ( RXR ) motifs that are required for
NGF- and
phosphoinositide - regulated
DOR export from intracellular compartments in neuroendocrine cells . -
pubmed
Sodium - glucose co - transporter 2 (
SGLT2 ) inhibitors inhibit
glucose re - absorption in the proximal renal tubules . -
pubmed
The search of the literature revealed many possible cardioprotective mechanisms , because
SGLT2 inhibitors ( i ) increase natriuresis and act as diuretics with unique properties leading to a reduction in preload and myocardial stretch ( the diuretic hypothesis ) ; ( ii ) decrease blood pressure and afterload ( the blood pressure lowering hypothesis ) , ( iii ) favor the production of
ketones , which can act as a ' superfuel ' in the cardiac and renal tissue ( the ' thrifty substrate ' hypothesis ) , ( iv ) improve many metabolic variables ( the metabolic effects hypothesis ) , ( v ) exert many anti - inflammatory effects ( the anti - inflammatory effects hypothesis ) , ( vi ) can act through the
angiotensin II type II receptors in the context of simultaneous
renin -
angiotensin -
aldosterone - system ( RAAS ) blockade leading to vasodilation and positive inotropic effects ( the RAAS hypothesis ) , ( vii ) directly decrease the activity of the upregulated in
heart failure Na +- H + exchanger in myocardial cells leading to restoration of mitochondrial
calcium handling in cardiomyocytes ( the
sodium hypothesis ) . -
pubmed
PATIENTS AND METHODS : : In this prospective comparative study , 32 eyes with
silicone oil tamponade of high viscosity ( 5700
CS ) and 32 normal fellow eyes were included . -
pubmed
CONCLUSIONS : : It seems that GAT overestimates IOP in eyes with high - viscosity
silicone oil compared with
NT , while both IOP measurement techniques in normal eyes provide similar values . -
pubmed
AChE activity in the cerebral cortex was lower in the infected group than in the control ; there was an increase in the activity of
NTPDase ,
5 '- nucleotidase and
ADA , possibly indicating greater hydrolysis of
ATP ( P & lt ; 0.001 ) ,
ADP ( P & lt ; 0.01 ) and
AMP ( P & lt ; 0.01 ) , followed by increased
adenosine deamination ( P & lt ; 0.001 ) . -
pubmed
In this project , an N - terminal labeling mass spectrometry method , terminal
amine isotopic labeling of substrates ( TAILS ) , was optimized for the in - depth analysis of
PHG and validated using a test
protease (
trypsin ) with known cleavage specificity . -
pubmed
,
aprepitant ) are known to exert , via the
NK-1R , an antitumor action ;
TNBC cells die by apoptosis . -
pubmed
At last , we detected stimulated by
retinoic acid gene 6 homolog (
STRA6 ) , a
cell surface receptor formerly known as a gene preventing podocytes from over - proliferative lesion induced by
HIV infection and was up - regulated by
retinoic acid , expressed at a much higher level in
SRNS kidneys . -
pubmed
Unfortunately , none of the biomarkers assessed were indicative of passing training , however ,
glucose , blood
urea nitrogen , and
creatine kinase (
CK ) levels stood out as abnormal . -
pubmed
Lipids in milk are secreted as a
triacylglycerol core surrounded by a trilayer membrane , the milk fat globule membrane (
MFGM ) . -
pubmed
We evaluated fasting blood -
glucose ( FBG ) , urinary
albumin /
creatinine ratio ( UACR ) ,
body weight , and kidney index ( KI ) in the different groups . -
pubmed
Initially , the use of Adam - Gibbs approach revealed that RIV 's molecular mobility ( below its glass transition temperature ) is significantly reduced in the presence of
SOL , while the use of
ATR - FTIR spectroscopy showed the formation of
hydrogen bonds ( HBs ) between the two
ASD components , indicating that these two mechanisms can be considered as responsible for system 's physical stability . -
pubmed
radiata , and found that
gibberellic acid (
GA ) significantly inhibited , whereas
paclobutrazol (
PBZ ) ,
abscisic acid (
ABA ) , and
ethrel promoted bulblet development , especially
PBZ , a
GA biosynthesis inhibitor . -
pubmed
Furthermore ,
GA reduced endogenous cytokinin (
CK ) content , as well as the activities of
carbohydrate metabolism enzymes , including
sucrose synthase (
SUS ) and
glucose-1 - phosphate adenylyltransferase (
AGPase ) , by downregulating the expression levels of
LrSUS1 ,
LrSUS2 , and genes encoding
AGPase large and small subunits . -
pubmed
PBZ had the opposite effect to
GA on
carbohydrate metabolism ; it decreased endogenous GA15 and
GA24 , thereby promoting bulblet development . -
pubmed
ABA promoted endogenous
auxin content and the activities of starch synthesis enzymes , especially soluble
starch synthase (
SSS ) and
granule - bound SS (
GBSS ) , through the up - regulation of the expression levels of
LrSS1 ,
LrSS2 , and
LrGBSS1 genes , which could also result in the accumulation of
carbohydrates in the bulblets and promote their development . -
pubmed
In addition ,
ethrel application partly promoted bulblet development by promoting endogenous
CK content . -
pubmed
Treatment of the
enzyme with
sodium dodecyl sulfate followed by
polyacrylamide gel electrophoresis gave a single band with a relative mobility suggesting a molecular weight of 76,000 plus and minus 2000 . -
pubmed
Sulfhydryl group modification of
thymidylate synthetase and its effect on activity and ternary complex formation . -
pubmed
Saturated and monoenoic iso ( C15 - C19 ) and
anteiso ( C15 , C17 ) fatty acids and saturated normal ( C14 -
C18 ) and
monoenoic normal ( C16 , C18 ) fatty acids were shown to be shown to be present by capillary gas chromatography - mass spectrometry . -
pubmed
[ Effect of
4 - hydroxypyrazolo [ 3,4 - d ] pyrimidine on
uricase activity ( author 's transl ) ] . -
pubmed
We investigated the ultrastructural localization of
annexin V a
Ca ( 2 +)-dependent phospholipid- and membrane - binding protein in the nervous system , heart , and skeletal muscles . -
pubmed
The observation that plasma membranes in cells expressing
annexin V have the protein associated with them is in agreement with previous data on
Ca ( 2 +)-dependent binding of the protein to brain and heart membranes , and on existence of both
EGTA- and
Triton X-100 - extractable and resistant fractions of
annexin V in these membranes . -
pubmed
An increase in CNS
dopamine neurotransmission , resulting from a competitive blockade of high - affinity
dopamine uptake mediated by both
D1 and
D2 dopamine receptors , is a primary determinant of the behavioral effects of
cocaine . -
pubmed
Anantin -- a peptide antagonist of the
atrial natriuretic factor (
ANF ) . -
pubmed
Protein chemical data and FAB -
MS have identified
anantin to be a cyclic polypeptide consisting of 17 common
L - amino acids . -
pubmed
The apparent differences between the
glutamate - induced current and nerve - evoked synaptic response revealed by
TI-233 can be explained by open - channel block of the
glutamate - activated ion - channel , and do not confute the hypothesis that
glutamate is the natural transmitter substance at this junction . -
pubmed
However , it is likely that
Ca channel in smooth muscle is not controlled by
cyclic AMP , in contrast to the cardiac muscle . -
pubmed
There are also different ( voltage- and
Ca - activated ) types of
K channel . -
pubmed
Endotoxaemia increased the rates of
lactate formation , at all levels of
insulin , both at 24 h ( approximately 32 % ) and 48 h ( approximately 26 % ) . -
pubmed
There are two
methionyl tRNA species in the cytoplasmic and mitochondrial fractions , one of which is unique to each fraction , indicating that the KtRNA fraction is free of CtRNA contamination . -
pubmed
KtRNA isolated by
DEAE chromatography or
agarose gel electrophoresis contains additional small RNAs which hybridize with both minicircle and maxicircle DNA . -
pubmed
The
anti - CD2 stimulation strongly potentiates the
PGE2 - induced
cAMP production while both
PMA and
A23187 produced a less potent effect . -
pubmed
On the opposite the
anti - CD2 treatment is without any effect on the basal
cAMP level contrasting with a marked increase of intracellular
cAMP concentrations with
A23187 or the combination of
A23187 and
PMA . -
pubmed
Phosphorylation of
chicken gizzard myosin :
myosin filament hypothesis of
calcium regulation . -
pubmed
ii )
ATP also induced disassembling of gizzard
myosin filaments . -
pubmed
iii ) Enzymic phosphorylation of
gizzard myosin light chains rendered both the
myosin conformation and the
myosin filaments resistant to the actions of
ATP . -
pubmed
iv ) Very high concentrations of
magnesium were required for formation of the
ATP - resistant filaments as well as for superprecipitation ( a model contraction ) of
actomyosin suspensions . -
pubmed
v )
ITP was a very poor substrate for
MLCK , and was accordingly incapable of inducing "
Ca - tension " in glycerinated fibers of gizzard muscle , but it did induce "
Mg - tension . " -
pubmed
Primarily from these findings , it was proposed that the mechanism of gizzard muscle contraction involves
ATP - induced changes in the morphology of
myosin filaments which are reversibly altered by enzymic phosphorylation and dephosphorylation of
myosin light chains in the presence of relatively high concentrations of
magnesium . -
pubmed
CR1 belongs to a group of proteins that contain a structural element characterized by its size of 60 - 65
amino acids , and four conservatively positioned
cysteines , which engage in a self - contained 1 - 3 , 2 - 4
disulphide arrangement . -
pubmed
This paper reports further optimization of the
PEI bonding chemistry in the preparation of
HP - IEC columns for
oligonucleotides and
tRNA species . -
pubmed
Synthesis of
chicken calcitonin - gene - related peptide (
cCGRP ) by application of
sulfoxide - directed
disulfide - bond - forming reaction . -
pubmed
Hepatitis B surface antigen (
HBsAg ) was detectable up to 3 weeks , while
hepatitis B e antigen (
HBeAg ) persisted only for 3 days in mosquitoes after feeding on
hepatitis B viral antigens - positive blood . -
pubmed
The
racemase has one
pyridoxal phosphate molecule per 42 - kDa subunit , has a Vmax of 3570 units / mg and a Km of 7.8 mM in the L to D direction , and has a Vmax of 1210 units / mg and a Km of 2.2 mM in the D to L direction . -
pubmed
The purified
enzyme is inhibited in a time - dependent manner by both
L - and
D -( l - aminoethyl ) phosphonates (
Ala - P ) , confirming observations of Atherton et al . -
pubmed
Thus ,
enzyme after exposure to
[ 14C ] Ala - P or
[ alpha-3H ] Ala - P and gel filtration contains stoichiometric amounts of radioactive label , but denaturation quantitatively releases intact
Ala - P into solution as revealed by high - performance liquid chromatography and cocrystallization with authentic material . -
pubmed
The
Ala - P isomers are slow binding inhibitors of this
racemase as is the
alpha , alpha '- dimethyl analogue but not the D or
L isomers of the corresponding
phosphinate.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
Properties of
SV 40 rescued from
actinomycin D - sensitive and
actinomycin D - resistant transformed hamster cells . -
pubmed
The potential effect of maternal
caffeine ingestion upon total brain protein and the concentration of two prototype neuropeptides ,
thyrotropin - releasing hormone (
TRH ) and its derivative ,
cyclo ( His - Pro ) in neonates was examined during the nursing period in the context of variable maternal protein intake . -
pubmed
By contrast , no significant alterations were observed in
TRH concentrations between
caffeine and control groups . -
pubmed
The
enzyme - inhibitor complex was not dissociated by 4 M
KCl . -
pubmed
To determine if this effect was due to
Ca2 + buffering , a mutant form of
calmodulin that has an eight -
amino acid deletion in the central helix ( CaM-8 ) was used . -
pubmed
The mutated
calmodulin binds
Ca2 + normally , but alters the ability to interact with known
Ca2 +/calmodulin - activated enzymes . -
pubmed
In vitro competition analysis using HIT cell extracts verified that in the presence of
Ca2 + ,
CaM-8 exhibited at least a 100 - fold lower affinity for
calmodulin - binding proteins than did normal
CaM in this model beta - cell . -
pubmed
This condition was progressive and characterized by elevated blood
glucose that coincided with reduced levels of pancreatic
insulin and low circulating serum
insulin levels . -
pubmed
Carbachol , which is thought to act in beta - cells through the release of intracellular
Ca2 + stores and activation of
protein kinase - C , restored both phases of secretion to normal levels . -
pubmed
These results suggest that disruption of intracellular
Ca2 + homeostasis alone is sufficient to interfere with the
insulin secretion pathway . -
pubmed
Dose linearity of the pharmacokinetics of the new
H +/ K (+)- ATPase inhibitor
pantoprazole after single intravenous administration . -
pubmed
Pantoprazole is a specific inhibitor of the
H +/ K (+)- ATPase of the gastric parietal cell . -
pubmed
The dose - independent elimination of
pantoprazole was attributed to the lack of interaction of the drug with
cytochrome P450 . -
pubmed
[ Clinical significance of
thyrotropin measurement by immunoradiometric assay and
thyrotropin - releasing hormone test ] . -
pubmed
The
GABAB receptor antagonist
p -( 3 - aminopropyl )- p - diethoxymethyl - phosphinic acid (
CGP 35348 ) abolished the inhibitory effect of an increase in
D - glucose by 60 and 100 mM on
[ 3H ] NE release but only tended to diminish the inhibition caused by an increase by 320 mM. -
pubmed
Arc -
L1 -
Arc protects operator DNA in a manner indistinguishable from that of wild - type
Arc in
DNase I and
copper -
phenanthroline footprinting studies , but the covalent attachment of the two monomers results in enhanced affinity for operator DNA . -
pubmed
INTERVENTION : Stepped - care therapy (
pravastatin ,
nicotinic acid ,
cholestyramine , and
gemfibrozil ) to decrease total
cholesterol levels to less than 4.1 mmol / L ( 160 mg / dL ) and the ratio of LDL
cholesterol to
HDL cholesterol to less than 2.0 . -
pubmed
RESULTS : Mean lipid levels at baseline were as follows : total
cholesterol , 5.5 mmol / L ( 214 mg / dL ) ;
LDL cholesterol , 3.6 mmol / L ( 140 mg / dL ) ;
HDL cholesterol , 1.1 mmol / L ( 42 mg / dL ) ; and
triglycerides , 1.8 mmol / L ( 159 mg / dL ) . -
pubmed
With
pravastatin , changes in levels from baseline were -22 % for total
cholesterol , -32 % for
LDL cholesterol +8 % for
HDL cholesterol , and -15 % for
triglycerides ( P & lt ; 0.001 for all comparisons ) . -
pubmed
-11 % for
LDL cholesterol , +8 % for
HDL cholesterol , and -10 % for
triglycerides ( P & lt ; 0.001 for all comparisons ) . -
pubmed
With 2.25 to 3 g of
nicotinic acid , these changes were -7 % for total
cholesterol ( P = 0.007 ) , -14 % for
LDL cholesterol ( P & lt ; 0.001 ) , +6 % for
HDL cholesterol ( P = 0.02 ) , and -13 % for
triglycerides ( P = 0.03 ) . -
pubmed
With
cholestyramine , total
cholesterol and
LDL cholesterol levels were unchanged compared with the previous step ; the change in
HDL cholesterol level was -8 % ( P = 0.03 ) ; and the change in
triglyceride level was +46 % ( P & lt ; 0.001 ) . -
pubmed
With
gemfibrozil , total
cholesterol level was unchanged ; the additional change in
LDL cholesterol level was +12 % ( P = 0.09 ) ; the change in
HDL cholesterol level was +12 % ( P = 0.03 ) ; and the change in
triglyceride level was -37 % ( P & lt ; 0.001 ) . -
pubmed
Pravastatin with
nicotinic acid and
pravastatin with
gemfibrozil are well - tolerated combinations that can maintain target
LDL cholesterol levels , decrease
triglyceride levels , and increase HDL
cholesterol levels . -
pubmed
In case 3 , the
plasma adrenocorticotropin level was unchanged during treatment in the first week , despite marked reductions in plasma and 24 - hour urinary free
cortisol levels . -
pubmed
Inhibition was found to require the coaggregation of the two receptors and to depend on the presence of a
tyrosine - based inhibition motif ( ITIM ) in the
intracytoplasmic domain of
Fc gammaRIIB . -
pubmed
We report here that the coaggregation with
Fc gammaRIIB does not prevent
Fc epsilonRI from triggering activation signals in BMMC and induces the
tyrosine phosphorylation of
Fc gammaRIIB . -
pubmed
At 1 week after
MI , circulating plasma levels of
adrenaline ,
angiotensin II ,
atrial natriuretic factor (
ANF ) and
vasopressin were significantly elevated . -
pubmed
For example , one
molecule that we have previously identified as a component of the IPM ,
laminin beta2 ( formerly known as
s - laminin ) , is implicated in the differentiation of rod photoreceptor cells . -
pubmed
Adrenal
steroids act through two receptor subtypes , the
glucocorticoid receptor (
GR ) and the
mineralocorticoid receptor , and activation of each receptor subtype has distinct biochemical and physiological consequences . -
pubmed
We have examined adrenal
steroid regulation of the
neurotrophins brain - derived neurotrophic factor ,
neurotrophin-3 , and
basic fibroblast growth factor , as well as the growth associated protein
GAP-43 , through activation of
GR or
mineralocorticoid receptor with selective agonists . -
pubmed
Thus the extent to which adrenal
steroids regulate hippocampal neurotrophic and growth - associated factors , appears to be dependent both on the
adrenal steroid receptor subtype activated and on the hippocampal subregion examined . -
pubmed
Multiple doses of
nicotine were tested and 0.5 mg / kg
nicotine , given on both training and testing days , improved contextual learning but had no effect on formation of an auditory
CS - US association . -
pubmed
The
nicotinic receptor antagonist
mecamylamine ( 1 and 2 mg / kg ) did not alter contextual fear conditioning but
mecamylamine did prevent the
nicotine - associated
increase in contextual learning . -
pubmed
A higher dose of
nicotine ( 1 mg / kg , training day only ) interfered with contextual conditioning when the context was paired with both the
CS and US , but had no effect on the auditory
CS - US association . -
pubmed
Here we have constructed
GFP - fusions of N- and C - domain and found that the N - terminal domain showed
CAP - specific enrichment at the anterior and posterior ends of cells like endogenous
CAP irrespective of the presence of the
proline rich region . -
pubmed
Effect of food on the pharmacokinetics of oral
MMI270B (
CGS 27023A ) , a novel
matrix metalloproteinase inhibitor . -
pubmed
MMI270B is a
matrix metalloproteinase inhibitor ( MMPI ) with in vitro and in vivo activity . -
pubmed
Functional studies demonstrate that
cAMP represses whereas
tumor necrosis factor - alpha , an activator of
NF - kappaB , stimulates promoter activity . -
pubmed
Consistently , inhibition by
cAMP is reverted by increasing intracellular
HATs and is completely abolished by site - specific mutagenesis of the
cAMP response element . -
pubmed
In contrast , specific mutations in the NF - kappaB response element abolish responsiveness to
p65 / RelA and
HATs without affecting
cAMP - dependent repression . -
pubmed
Niemann - Pick type C disease is characterized by the accumulation of
cholesterol and other lipids within the lysosomal compartment , a process that is often accompanied by a reduction in
acid sphingomyelinase activity . -
pubmed
These data suggest that
cholesterol - induced inhibition is a posttranslational event , perhaps involving cofactor mediated modulation of enzymatic activity or alterations in
acid sphingomyelinase protein trafficking and maturation . -
pubmed
This study investigated whether the exaggerated response to
carbon dioxide in
panic disorder (
PD ) can be modulated by antagonists of
muscarinic cholinergic receptors . -
pubmed
The
KOR gene encodes kappa(1)-sites , which specifically bind
arylacetamide compounds , and the possible existence of
kappa - opioid receptor subtypes derived from another kappa - opioid - receptor gene , yet to be characterized , remains a very contentious issue . -
pubmed
kappa ( 2 )- Opioid receptors are described as binding sites typically labelled by the non - selective
benzomorphan ligand
[ 3H ] bremazocine in the presence of
mu - , delta - and kappa ( 1 )- opioid receptor blocking ligands . -
pubmed
Scatchard analysis showed that 68+/-12 % of the binding sites arise from the
MOR gene , 27+/-1 % from the
DOR gene and 14.5+/-0.2 % from the
KOR gene , indicating that the three known genes account for total [ 3H]
bremazocine binding . -
pubmed
MEASUREMENTS AND MAIN RESULTS : We measured changes in haemodynamic variables , dose of
norepinephrine required to maintain a mean arterial pressure greater than 70 mmHg and plasma concentrations of
complement anaphylatoxins and several
cytokines . -
pubmed
Salivary non - immunoglobulin agglutinin inhibits
human leukocyte elastase digestion of acidic
proline - rich salivary proteins . -
pubmed
However ,
human leukocyte elastase , arriving in gingival exudates from inflamed periodontal tissues , degrades the acidic
proline - rich salivary proteins , preventing binding to
hydroxylapatite surfaces . -
pubmed
Here it is reported that high - molecular - weight non - immunoglobulin salivary
agglutinin inhibited the proteolytic action of
human leukocyte elastase on purified acidic
proline - rich salivary proteins . -
pubmed
The addition of antibody against salivary
agglutinin blocked the inhibitory effect of parotid saliva on exogenously applied
human leukocyte elastase , allowing for the
elastase - mediated digestion of the salivary acidic
proline - rich salivary proteins . -
pubmed
Salivary
agglutinin , therefore , is a physiologically important inhibitor of
human leukocyte elastase and is able to inhibit
elastase - mediated digestion of salivary acidic
proline - rich proteins . -
pubmed
IgE - mediated
anaphylaxis after first intravenous infusion of
cyclosporine . -
pubmed
Gamma - TQ released mitochondrial
cytochrome c to the cytosol , induced the cleavage of
poly ( ADP - ribose ) polymerase , and depleted intracellular
glutathione . -
pubmed
Unlike xenobiotic electrophiles ,
gamma - TQ is a highly cytotoxic arylating electrophile that stimulates apoptosis in several
cancer cell lines including cells that express
MDR through both
P - glycoprotein and
MRP - associated proteins . -
pubmed
Serial
Dexamethasone Suppression Tests and Clinical Outcome in
ECT . -
pubmed
The
Ca ( 2 +)/ calmodulin - dependent protein phosphatase ,
calcineurin , modulates a number of key
Ca ( 2 + ) signaling pathways in neurons , and has been implicated in
Ca ( 2 +)-dependent negative feedback inactivation of
N - methyl - D - aspartate receptors and voltage - sensitive
Ca ( 2 + ) channels . -
pubmed
In contrast , we report here that three mechanistically disparate
calcineurin inhibitors ,
FK-506 ,
cyclosporin A , and the
calcineurin autoinhibitory peptide , inhibited high - voltage - activated
Ca ( 2 + ) channel currents by up to 40 % in cultured hippocampal neurons , suggesting that
calcineurin acts to enhance
Ca ( 2 + ) currents . -
pubmed
The immunosuppressant ,
rapamycin , and the
protein phosphatase 1 / 2A inhibitor ,
okadaic acid , did not decrease
Ca ( 2 + ) channel current , showing specificity for effects on
calcineurin . -
pubmed
FK-506 also inhibited substantially more
Ca ( 2 + ) channel current in 4 - week - old vs. 2 - week - old cultures , an effect paralleled by an increase in
calcineurin A mRNA levels . -
pubmed
Identification of a non - canonical
tyrosine - based endocytic motif in an
ionotropic receptor . -
pubmed
Three
amino acids in the C terminus of
P2X ( 4 ) (
Tyr(378 ) ,
Gly(381 ) , and
Leu(382 ) ) compose a non - canonical
tyrosine - based sorting signal of the form YXXGL . -
pubmed
Mutation of either the
tyrosine - binding pocket of the
mu2 subunit of
adaptor protein-2 or the YXXGL motif in the receptor C terminus caused a decrease in receptor internalization and a dramatic increase in the surface expression of
P2X ( 4 ) receptors . -
pubmed
The YXXGL motif represents a non - canonical
tyrosine - based sorting signal that is necessary for efficient endocytosis of the
P2X ( 4 ) receptor . -
pubmed
Preclinical evaluation of a radiosensitizing effect of
gemcitabine in
p53 mutant and
p53 wild type
bladder cancer cells . -
pubmed
DNA damage was evaluated by flow cytometric assessment of
poly ( ADP - ribose ) polymerase , and apoptosis was determined by terminal - deoxynucleotidyltransferase - mediated
dUTP nick - end labeling and flow cytometric assessment after
annexin - V and
propidium iodide labeling . -
pubmed
Gemcitabine alone had a dose - dependent cytotoxic and apoptosis inducing effect on all TCC cell lines independent of
p53 status . -
pubmed
CONCLUSIONS :
Gemcitabine is effective in TCC cell lines independent of
p53 status . -
pubmed
Both chains were labeled with two different tags : a (
His)(6 ) was introduced at the C - terminal end of
alpha chain , while beta chain was terminated by
c - myc . -
pubmed
Since an isolated
alpha chain is unstable unless it is associated with a beta chain , this design permits rapid separation of alpha , beta - heterodimer from unpaired beta chain in a single step of
Ni - NTA Agarose chromatography yielding 90 % pure
alpha , beta - TCR . -
pubmed
Regular drug use ( use at least once a month ) other than cannabis whilst a
VDP was found to be highest among males , with regular
ecstasy use reported by 4 % and regular
cocaine use reported by 2 % . -
pubmed
The total
cholesterol concentration and
CK ,
AST and
LDH activities in the serum showed higher values than those of control calves . -
pubmed
Additional specimens were prepared and examined with
TEM using a
silver nitrate - staining technique . -
pubmed
When bonded according to manufacturer 's directions ,
Panavia F produced bond strengths that were significantly lower than Bistite
II DC ( p<0.05 ) . -
pubmed
The placement of an additional layer of a LVBR improved significantly the bond strengths of
Panavia F ( p<0.05 ) , but not of Bistite
II DC ( p>0.05 ) . -
pubmed
Phosphorylated amino acids : model compounds for solid - state
31P NMR spectroscopic studies of
proteins . -
pubmed
The cross - polarization buildup rates and longitudinal relaxation times of
31P and
1H were - determined and compared with the values measured for a
triphosphate (
GppCH2p ) bound to a crystalline
protein (
Ras ) . -
pubmed
Both
mGluR1 and
mGluR5 mediate
Ca2 + release and inward currents in hippocampal
CA1 pyramidal neurons . -
pubmed
The selective
group I mGluR agonist ,
( RS )- 3,5 - dihydroxyphenylglycine (
DHPG ) , evoked a transient increase in intracellular
Ca2 + levels ( [
Ca2+]i ) , within neuronal
somas and apical dendrites , together with a relatively long lasting inward current ( I(
DHPG ) ) . -
pubmed
The selective
mGluR1 and
mGluR5 antagonists ,
( S )-(+)- alpha - amino - a - methylbenzeneacetic acid (
LY367385 ; 100 microM ) and
2 - methyl-6 -( phenylethynyl )- pyridine (
MPEP;10 microM ) , respectively , markedly inhibited both I(
DHPG ) and the
DHPG - evoked increase in [
Ca2+]i . -
pubmed
Moreover , these antagonists inhibited the
Ca2 + response by more than 50 % suggesting a synergistic interaction between
mGluR1 and
mGluR5 . -
pubmed
This study demonstrates that in
CA1 pyramidal neurons group I mGluR - mediated inward currents and
Ca2 + release from intracellular stores are enhanced under depolarising conditions and that
mGluR1 and
mGluR5 both contribute to these phenomena . -
pubmed
N -
SasA adopts a canonical
thioredoxin fold but lacks the catalytic
cysteine residues . -
pubmed
Our analysis suggests that the structural differences between N -
SasA and
KaiB are the result of only a few critical
amino acid substitutions . -
pubmed
[ Influences of
FVP1 on the curative and negative effects of
CTX ] . -
pubmed
OBJECTIVE : To observe the influences of
FVP1 on both curative and negative effects of
CTX . -
pubmed
Site - specific vectorial immobilization of
YCC via
Cys102 on single - walled
carbon nanotubes ( SWNT ) thus provides a selective interface between nanoscopic electronic devices and complex enzymes . -
pubmed
Cytokine - induced
prostaglandin E2 production and
cyclooxygenase-2 expression in dental pulp cells : downstream
calcium signalling via activation of
prostaglandin EP receptor . -
pubmed
AIM : To determine whether ( i ) proinflammatory
cytokines stimulate
prostaglandin E ( 2 ) (
PGE ( 2 ) ) production and
cyclooxygenase (
COX ) gene expression in dental pulp cells , and ( ii ) pulp cells that express different
prostaglandin E ( 2 ) receptor (
EP ) isoforms and their activation by
PGE ( 2 ) leads to downstream
Ca ( 2 + ) signalling . -
pubmed
Expression of
prostaglandin EP receptor isoforms was studied by RT - PCR , whereas
fura-2 fluorescence was used to measure
calcium mobilization . -
pubmed
CONCLUSIONS :
Interleukin-1beta and
TNF - alpha may stimulate
PGE ( 2 ) production in dental pulp cells . -
pubmed
Activation of
prostaglandin EP receptors in dental pulp cells by
PGE ( 2 ) may induce
Ca ( 2 + ) signalling to regulate cellular biological activity during
inflammation . -
pubmed
BACKGROUND : The integral synaptic vesicle protein and putative
calcium sensor ,
synaptotagmin 1 (
STG ) , has also been implicated in synaptic vesicle ( SV ) recovery . -
pubmed
The candidate biomarker was then obtained from
tricine one - dimensional
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis by matching the molecular weight with peaks on WCX2 chips and was identified as
Serum Amyloid A protein (
SAA ) by MALDI / MS - MS and database searching . -
pubmed
Urinary
trefoil factor 1 is a novel potent inhibitor of
calcium oxalate crystal growth and aggregation . -
pubmed
We searched for direct evidence to define the inhibitory activity of urinary
trefoil factor 1 against
calcium oxalate crystal growth . -
pubmed
Moreover , we also evaluated whether urinary
trefoil factor 1 has any effects on
calcium oxalate crystal aggregation and transformation . -
pubmed
MATERIALS AND METHODS : Isolated and aggregated forms of
calcium oxalate monohydrate crystals were produced in the absence or presence of 7 , 70 and 700 ng / ml urinary
trefoil factor 1 ,
nephrocalcin as a positive control or
lysozyme ( Sigma - Aldrich ) as a negative control . -
pubmed
RESULTS : The data clearly indicated that urinary
trefoil factor 1 and
nephrocalcin at physiological levels could effectively inhibit
calcium oxalate monohydrate crystal growth and aggregation , whereas
lysozyme did not affect the growth and aggregation of
calcium oxalate monohydrate crystals . -
pubmed
At a supraphysiological concentration of 4 microg / ml urinary
trefoil factor 1 and
nephrocalcin could transform
calcium oxalate monohydrate crystals to the
dihydrate type , which has much less adsorptive capability . -
pubmed
CONCLUSIONS : To our knowledge these data provide the first direct evidence that urinary
trefoil factor 1 is a novel potent inhibitor of
calcium oxalate crystal growth and aggregation , and can transform
calcium oxalate monohydrate crystals to the
dihydrate type . -
pubmed
Norepinephrine increased maternal heart rate (
HR ) , mean arterial pressure ( MAP ) and uterine vascular resistance ( R(UtA ) ) , and decreased uterine volume blood flow ( Q(UtA ) ) . -
pubmed
The authors have investigated whether
glaucoma and filtration surgery are associated with increased levels of
vascular endothelial growth factor (
VEGF ) , and whether a humanized monoclonal antibody against
VEGF ,
bevacizumab , can reduce postoperative
scar formation and improve surgical outcome . -
pubmed
The effect of
VEGF and
bevacizumab on Tenon fibroblasts in vitro was determined using a proliferation assay . -
pubmed
Fibroblast proliferation in vitro was stimulated by delivery of
VEGF , and was inhibited by administration of
bevacizumab . -
pubmed
The helical propensity of a peptide corresponding to
beta2 - strand of
salivary SA cystatin analyzed by CD display high helical propensity in aqueous solution , whereas peptides matching the
beta2 - strand
amino acid sequence of
cystatins S and
SN , display random
coil conformation in aqueous solution but acquire alpha - helical conformation in the presence of
trifluoroethanol (
TFE ) . -
pubmed
The specific inhibitor of
VAP-1 ,
UV-002 , was administered by daily i.p . -
pubmed
,effects on
insulin secretion and sensitivity and blood
glucose levels were assessed by means of a
hyperglycaemic glucose clamp , meal tolerance test , homeostasis model assessment -
insulin resistance score , and daily self - monitoring of blood
glucose . -
pubmed
RESULTS :
Palosuran did not affect second - phase
insulin response ( primary end - point ) during the
hyperglycaemic glucose clamp in comparison with placebo [ paired difference of -1.8 microU ml(-1 ) , 95 % confidence interval ( CI ) -7.8 , 4.2 ] . -
pubmed
Likewise , no effects of
palosuran were detected on the first - phase
insulin response , or on
insulin secretion and blood
glucose levels during the meal tolerance test or on homeostasis model assessment -
insulin resistance score . -
pubmed
The fingerprinting atlas indicated that , compared with those without
SCI , the
SCI group demonstrated the following characteristics with regard to second principal component : it is made up of
fatty acids ,
myc - inositol ,
arginine ,
very low - density lipoprotein (
VLDL ) ,
low - density lipoprotein (
LDL ) ,
triglyceride (
TG ) ,
glucose , and
3 - methyl - histamine . -
pubmed
Both mitochondrial membrane potential ( MMP ) and
oxygen consumption were assessed in conjunction with quantification of mitochondrial DNA and mitochondrial biogenesis - related factors , such as
peroxisome proliferator - activated receptor - gamma coactivator 1 alpha (
PGC1alpha ) ,
nuclear respiratory factor 1 (
NRF1 ) and
mitochondrial transcription factor A (
Tfam ) , by real - time
polymerase chain reaction . -
pubmed
[ Effect of
rapamycin combined with
cisplatin on head and neck squamous
cancer cells regulated by
CCL19 ] . -
pubmed
OBJECTIVE : To investigate the synergistic effects of
rapamycin and
cisplatin on head and neck squamous
cancer cells regulated by
chemokine ( C - C motif ) ligand 19 (
CCL19 ) . -
pubmed
RESULTS :
Rapamycin and
cisplatin could respectively increase the growth arrest , the proportion of G(1 ) phase and apoptosis of
CCL19 induced
cancer cells ( P & lt ; 0.05 ) . -
pubmed
AIM : The aim of this study was to assess a spray formulation containing colloidal
silver and
hyaluronic acid . -
pubmed
The fact that the K(m ) of OTxA for the efflux transporter
hNPT4 was much higher than those for the uptake transporters
hOAT1 and
hOAT3 may favor the accumulation of
OTxA in the tubular cell and lead to
nephrotoxicity . -
pubmed
The results indicated that : ( 1 ) the decay in fluorescence was linearly dependent on the concentration of
NO ( 2 ) , indicating that protein oxidation was first order with respect to
NO ( 2 ) concentration in both BALF and in buffered
albumin solution ; ( 2 ) the depletion kinetics of
protein in BALF was non - linear with respect to substrate concentration ; ( 3 ) the rate of protein depletion was much slower in BALF than in a buffered solution of
albumin , suggesting that the presence of antioxidants in BALF protected
proteins from being oxidized by
NO ( 2 ) ; and ( 4 ) whereas the addition of
ascorbic acid to buffered
albumin solution significantly attenuated
albumin depletion , the addition of
glutathione had no effect . -
pubmed
For
gp120 sequence analysis ,
nucleotide mixtures were considered when the second highest electropherogram peak was & gt;25 % ; sequences were translated into all possible permutations and classified as X4 , dual / mixed ( DM ) , and R5 based on coincident ESTA results . -
pubmed
According to multivariate analysis ,
amino acid changes at 9 and 7 positions within the
C1 to
C4 and
V1 to V5 regions , respectively , maintained a statistical significance , as did the net charge of V3 and
C4 . -
pubmed
Furthermore , using quantitative electrophoretic mobility - shift assays we validated the positional interdependence of two
nucleotides and the presence of a secondary Sox motif in the affinity landscape of
Sox4 . -
pubmed
These results suggest that a concerted rearrangement of two interface
amino acids enables
Sox4 to accommodate primary and secondary motifs . -
pubmed
Phosphorylation of mycobacterial
PcaA inhibits
mycolic acid cyclopropanation : consequences for intracellular survival and for phagosome maturation block . -
pubmed
Here , we demonstrated that
mycolic acid cyclopropane synthase PcaA , but not
MmaA2 , was phosphorylated by
mycobacterial Ser / Thr kinases at
Thr-168 and
Thr-183 both in vitro and in mycobacteria . -
pubmed
In contrast to the wild - type and phosphoablative
pcaA alleles , introduction of the phosphomimetic
pcaA allele in the
DeltapcaA mutant failed to restore the parental
mycolic acid profile and cording morphotype . -
pubmed
Chronic treatment with
AG825 or
AG1478 , selective inhibitors of
erbB2 and
EGFR respectively , did not affect
hyperglycemia but led to an exacerbation whereas acute administration of the
EGFR ligand ,
epidermal growth factor (
EGF ) , led to an improvement in cardiac recovery in
diabetic hearts . -
pubmed
Losartan treatment improved cardiac function in
diabetes but also impaired
EGFR phosphorylation in
diabetic heart . -
pubmed
Co - administration of
EGF rescued
Losartan - mediated reduction in
EGFR phosphorylation and significantly improved cardiac recovery more than with either agent alone . -
pubmed
The sensor responded to
maltose and
maltotriose and the response was completely abolished by introduction of a single point mutation in the BRET(2 ) tagged
MBP protein . -
pubmed
The biosensor 's estimate of
maltose in beer matched that of a commercial
enzyme - linked assay but was quicker and more precise , demonstrating its applicability to real - world samples . -
pubmed
kansui led to the isolation of 12 diverse
terpenoids whose structures were identified by
( 1 ) H ,
( 13 ) C NMR spectroscopy and ESI -
MS as
kansuinine A ( 1 ) ,
kansuinine B ( 2 ) ,
kansuinine C ( 3 ) ,
kansuiphorin C ( 4 ) ,
3 - O -( 2'E,4'Z - decadienoyl )- 20 - O - acetylingenol ( 5 ) ,
3 - O -( 2'E,4'Edecadienoyl )- 20 - O - acetylingenol ( 6 ) ,
3 - O -( 2'E,4'Z - decadienoyl )- 20 - deoxyingenol ( 7 ) ,
3 - O - benzoyl-20 - deoxyingenol ( 8) ,
5 - O - benzoyl-20 - deoxyingenol ( 9 ) ,
kansenone ( 10 ) ,
epi - kansenone ( 11 ) ,
euphol ( 12 ) . -
pubmed
Specifically , we report formulation optimisation of SLMH microparticles and tablets using
coumarin 102 ( log P = 4.09 ) as a model Biopharmaceutics Classification System class
II drug . -
pubmed
Binding of the antibody to bone marrow - derived mast cells triggered activation events that included cell degranulation ,
Ca ( 2 + ) response , dephosphorylation of
ezrin / radixin / moesin ( ERM ) family proteins , and potent
tyrosine phosphorylation of the
non - T cell activation linker (
NTAL ) but only weak phosphorylation of the linker for activation of T cells (
LAT ) . -
pubmed
To construct such an in situ amplification system , streptavidin - labeled gold nanoparticles ( AuNP -
SA ) were initially used for the labelling of initiator strands ( S0 ) and detection antibody ( mAb2 ) with a large ratio ( mAb2 - AuNP - S0 ) , and then two auxiliary DNA strands
S1 and
S2 were designed for in situ propagation of DNAzyme concatamers with the
hemin / G - quadruplex format . -
pubmed
Myocardial blood flow and high - energy
nucleotides were obtained in the
LAD region and normalized to remote regions . -
pubmed
Despite reduced regional blood flows in HIB hearts ,
ATP content in the
LAD region , when normalized to the remote region was similar in HIB versus SWOP ( 1.06 + -0.06 and 1.02 + -0.05 respectively ;
NS ) as was the transmural
phosphocreatine (
PCr ) to
ATP ratio ( 2.1 + -0.2 and 2.2 + -0.2 respectively ;
NS ) . -
pubmed
Emerging
low - density lipoprotein (
LDL ) therapies : Management of severely elevated LDL
cholesterol -- the role of LDL - apheresis . -
pubmed
Based on electrochemical principles , it selectively removes
apolipoprotein B - containing lipoproteins through extracorporeal precipitation with either
heparin (
Heparin - induced Extracorporeal LDL Precipitation , ie , HELP ) or
dextran sulfate ( Liposorber ) . -
pubmed
LDL - apheresis can lead to an acute decrease in
LDL cholesterol (
LDL - C ) of 70%-80 % , but there is a rapid rebound to baseline levels within approximately 2 weeks . -
pubmed
Reducing the production cost of
cellulase as the key enzyme for cellulose hydrolysis to fermentable
sugars remains a major challenge for biofuel production . -
pubmed
The requirement for long - lasting artificial ventilation can be reduced with an
oxime therapy that is optimized by using the
cholinesterase status for guidance or by measures ( e.g. -
pubmed
CONCLUSION : Our economic analysis shows that although the costs of the two
trastuzumab plus
taxane regimens are similar , they may contribute to the on - going debate about the availability and use of innovative chemotherapy drugs , in particular in
human epidermal growth factor receptor 2 - positive metastatic
breast cancer with new therapies such as
trastuzumab - DM1 and
pertuzumab . -
pubmed
miR-181b restricted
cAMP production by
post - transcriptionally downregulating
AC9 expression . -
pubmed
Herein , we have successfully formulated insoluble iodinated
cisplatin (
CDDP - I ) into an
LPI NPs ( lipid - coated iodinated
CDDP nanoparticles ) . -
pubmed
On days 14 , 28 and 42 of exposure to
ZEN ( 100 mug kg(-1 ) feed day(-1 ) ) , apoptosis in
IPP GC was evaluated histologically in
HE - stained specimens , immunohistochemically by
active caspase-3 staining and in mononucleosome and oligonucleosome detection - based ELISA . -
pubmed
Proliferation was evaluated histologically by mitosis detection in
HE - stained specimens , immunohistochemically by
PCNA staining and in the
MTT tetrazolium salt colorimetric assay detecting mitogenic responses of B cells to LPS . -
pubmed
Exposure to low doses of
ZEN for several weeks intensified apoptosis and weakened proliferation in
IPP lymphocytes . -
pubmed
ZEN influences gut - associated lymphoid tissue (
GALT ) by decreasing the expression of
CD21 + on B cells and by increasing the percentage of B1 cell populations . -
pubmed
Tiotropium is a
muscarinic receptor antagonist , which is taken once daily , in maintenance therapy , in every stage of the disease progress . -
pubmed
The putative
diol synthase from G. cingulate was purified by
His - Trap affinity chromatography with a specific activity of 0.87 U mg(-1 ) , an eightfold purification , and a yield of 28 % . -
pubmed
One unit of activity was defined as the amount of
enzyme required to produce 1 mumol of
7,8 - dihydroxy-9,12 ( Z , Z )- octadecadienoic acid (
7,8 - DiHODE ) per min . -
pubmed
The
enzyme converted
linoleic acid to a product , identified as
7S,8S - DiHODE by liquid chromatography - mass spectrometry / mass spectrometry ( LC -
MS /
MS ) and nuclear magnetic resonance ( NMR ) spectroscopy . -
pubmed
The specific activity and catalytic efficiency ( k cat / K m ) of
7,8 - diol synthase from G. cingulate for the conversion of
fatty acid to
dihydroxy fatty acid followed the order
linoleic acid & gt ;
alpha - linolenic acid & gt ;
oleic acid & gt ;
palmitoleic acid , indicating that the
enzyme is a
7,8 - linoleate diol synthase (
7,8 - LDS ) . -
pubmed
The activity of the
enzyme for the conversion of
7,8 - DiHODE from
linoleic acid was maximal at pH 6.5 , 40 C , and 2.5 % ( v / v )
dimethyl sulfoxide (
DMSO ) . -
pubmed
The accumulation of
8 - HPODE was due to a higher
8 - dioxygenase activity in the N - terminal domain than
hydroperoxide isomerase activity in the C - terminal domain . -
pubmed
Stabilization of
HIF-1alpha by
FG-4592 promotes functional recovery and neural protection in experimental
spinal cord injury . -
pubmed
Combination therapy including the specific
HIF-1alpha blocker
YC-1 down - regulated the
HIF-1alpha expression and partially abolished the protective effect of
FG-4592 . -
pubmed
Taken together , our results revealed that the role of
FG-4592 in
SCI recovery is related to the stabilization of
HIF-1alpha and inhibition of apoptosis . -
pubmed
Modulation of
Cav3.2 T - type
calcium channel permeability by
asparagine - linked glycosylation . -
pubmed
Notably ,
Lys47 , a key residue in the bioactive peptide
lactoferricin , was particularly susceptible to modification . -
pubmed
The second study was a Phase
II dose - finding study of
senicapoc ( a Gardos channel blocker ) compared to placebo . -
pubmed
Compared to the placebo group the high dose
senicapoc showed significant improvement in change in
hemoglobin level , number and proportion of dense red blood cells , red blood cell count and indices and hematocrit . -
pubmed
While the Phase
II and the prematurely terminated phase III studies of
senicapoc showed that the drug improved red cell survival ( depending on dose ) , this did not lead to fewer painful crises . -
pubmed
In most cases , the anti -
TNF drug involved was
etanercept . -
pubmed
In 71 cases there was at least a partial resolution by discontinuation of the anti -
TNF treatment , initiation of
steroids or both . -
pubmed
A 90 - day repeated - dose oral toxicological evaluation was conducted according to
GLP and OECD guidelines on the
methylurate purine alkaloid theacrine , which is found naturally in certain plants . -
pubmed
Site - directed mutagenesis of eight transcription factor binding sites within
PGhGDSL demonstrated that the MYB1AT motif ( AAACCA ) at -603/-598 was critical for the 19
DPA - specific reporter gene expressions . -
pubmed
Analysis of genes co - expressed with
GhMYB1 showed that it potentially regulates a number of other 19 - 25
DPA - specific genes in networks including those functioning in the cell wall and precursor synthesis , but not the major polysaccharide and protein components of the fibre SCW . -
pubmed
The capabilities of
C18 and
styrene divinylbenzene reversed - phase sulfonated ( SDB - RPS ) Empore disks for capturing non - polar and polar metabolites , respectively , were compared . -
pubmed
High -
sucrose diet (
HSD ) fed adult females developed systemic
insulin resistance and exhibited an ovarian phenotype characterized by excess accumulation of lipids and
cholesterol in the ovary , decreased ovary size , and impaired egg maturation . -
pubmed
In this study , we prepared alginate microparticles loaded with the model antigen
fluorescein isothiocyanate conjugated -
bovine serum albumin . -
pubmed
The use of ovarian suppression combined with an
aromatase inhibitor
exemestane for 5 years , resulted in further reduction of
recurrence . -
pubmed
The results revealed drug - dependent and vegetable - dependent pharmacokinetic differences and indicated that ephemeral vegetables could have high capacity accumulating antibiotics ( up to 160 mug g-1 for
TC and 38 mug g-1 for
SMX ) within hours . -
pubmed
Based on the volume of distribution ( Vss ) ,
SMX was 3 - 6 times more extensively distributed than
TC . -
pubmed
Detailed analyses revealed that the
mTORC1 and
p53 signaling pathways are main targets of
curcumin . -
pubmed
In this study , the effect of therapeutic
hypervolemia ,
hemodilution , and
hypertension (
HHH - therapy ) on cerebral blood flow (
CBF ) was assessed by
xenon - enhanced computerized tomography ( XeCT ) hypothesizing an increase in
CBF in poorly perfused regions . -
pubmed
CBS treatment also significantly decreased the levels of
triglyceride , total
cholesterol , and free
fatty acid in the liver . -
pubmed
The activity of
superoxide dismutase in the liver was increased after treatment with
CBS , however , levels of
malondialdehyde and reactive
oxygen species decreased . -
pubmed
The expression of
fatty acid synthase , nuclear factor kappa - light - chain - enhancer of activated B cells ,
Cysteinyl aspartate - specific proteinase-3 , and synonyms
B - cell leukemia / lymphoma-2 gene - associated X protein were downregulated after treatment with
CBS , whereas the expression of
nuclear factor erythroid-2 - related factor 2 was upregulated . -
pubmed
Bioinspired ,
Manganese - Chelated Alginate -
Polydopamine Nanomaterials for Efficient in Vivo
T1 - Weighted Magnetic Resonance Imaging . -
pubmed
Hence , the
T1 relaxivity measurement showed that
PDA - complexed synthetic NGs reveal a strong positive contrast enhancement with r1 = 12.54 mM-1 s-1 in 7.0 T MRI images , whereas DA - complexed synthetic NGs showed a relatively lower
T1 relaxivity effect with r1 = 10.13 mM-1 s-1 . -
pubmed
Furthermore ,
T1 - weighted MRI results revealed that
PEGylated AlgPDA(
Ca /
Mn ) NGs significantly enhanced the signals from liver and
tumor tissues . -
pubmed
Therefore , owing to the enhanced permeability and retention effect , significantly enhanced in vitro and in vivo imagings , low cost , and one -
pot synthesis method , the
Mn - based biomimetic approach used in this study provides a promising and competitive alternative for noninvasive
tumor detection and comprehensive anatomical diagnosis . -
pubmed
Subsequent
periodic acid - Schiff ( PAS ) staining after diastase digestion confirmed
GH . -
pubmed
In this context , we have developed a hypothesis that describes the evolution of
schizophrenia - from the premorbid through the
prodromal stages to
syndromal psychosis - and posits dysregulation of
glutamate neurotransmission beginning in the
CA1 region of the hippocampus as inducing attenuated
psychotic symptoms and initiating the transition to
syndromal psychosis . -
pubmed
The
curcuminoid - imprinted polymer ( Cur -
MIP ) was synthesized from 0.0237 mmol of
styrene , 47.0 g of
acetonitrile , 1.0238 mmol of
ethylene glycol dimethacrylate , 0.0325 mmol of
curcuminoids , and 0.2480 mmol of
benzoyl peroxide . -
pubmed
The sample solution was separated using the Cur -
MIP via solid - phase extraction and analyzed on a Brownlee analytical
C18 column ( 150 mm x 6 mm , 5 microm ) using an isocratic elution consisting of
acetonitrile and 0.1 %
trichloroacetic acid ( 40:60 , v / v ) . -
pubmed
Thus , we concluded that the
Cur -
MIP and high - performance liquid chromatographic - fluorescence method could be applied to selective extraction and could be used as a rapid tool for the determination of
curcuminoids in medicinal herbal extracts . -
pubmed
In this study , we demonstrated that there are large species differences in the kinetics of interactions of a key class of antiviral drugs ,
acyclic nucleoside phosphonates (
ANPs ) , with
organic anion transporter 1 [ (
OAT1 )
SLC22A6 ] and identified a key
amino acid residue responsible for these differences . -
pubmed
Chimeric and site - directed mutagenesis studies along with comparative structure modeling identified
serine at position 203 ( S203 ) in
hOAT1 as a determinant of its lower Km value . -
pubmed
The studied parameters were air pollutants ,
ascorbic acid , photosynthetic pigments , leave 's pH , relative water content ,
proline ,
carbohydrates , and
hydrogen peroxide , in three chosen
spots , near the pollution source (
P1 ) , opposite the latter
spot (
P2 ) , and in an area relatively far from the source of contamination and which was chosen as the control site ( Ctrl ) . -
pubmed
The results showed in
P1 detection of air pollutants higher of about 80 % than in Ctrl , modifications in stress markers : increased concentration of the reactive
oxygen species "
hydrogen peroxide , " rise in the concentration of the osmoregulator
amino acid "
proline , " and depletion in
chlorophyll content , in contrast to an increase in
pheophytin . -
pubmed
Association between circadian gene
CLOCK and
cisplatin resistance in
ovarian cancer cells : A preliminary study . -
pubmed
The present study aimed to observe the expression of circadian gene
clock circadian regulator (
CLOCK ) in
ovarian cancer cells and the effects of circadian gene
CLOCK on
cis - dichlorodiamine platinum (
cisplatin ) resistance in
ovarian cancer cells . -
pubmed
The expression of circadian gene
CLOCK in
ovarian cancer cells was strongly associated with
cisplatin resistance . -
pubmed
The upregulation of circadian gene
CLOCK in
ovarian cancer cells may reduce its sensitivity to
cisplatin treatment . -
pubmed
Imatinib , a small molecule
tyrosine kinase inhibitor , induces rapid and sustained clinical benefit by inhibiting a number of signaling pathways , including
BCR -
ABL and
c - KIT . -
pubmed
RESULTS : There were 791 patients with the following characteristics : mean age 56.2 , male 52.3 % , known
diabetic 61.6 % , on
insulin 46.1 % , mean initial
glucose 50.0 dl / mg ( 2.8 mmol / L ) , from home 56.3 % . -
pubmed
Results : In HFD maternal plasma / serum , concentrations of
glucose ,
beta - hydroxybutyrate ,
branched - chain amino acids , and
leptin were increased , whereas those of
triacylglycerol ,
cholesterol , and
palmitic , oleic , linoleic , and alpha - linolenic acids were decreased . -
pubmed
Two new
indium sulfate tellurites , namely ,
In2 ( SO4 )( TeO3 )( OH ) 2 ( H2O ) and
In3 ( SO4 )( TeO3 ) 2F3 ( H2O ) , were synthesized by hydrothermal method in a one -
pot reaction . -
pubmed
In2 ( SO4 )( TeO3 )( OH ) 2(
H2O ) crystallized in centrosymmetric (
CS ) space group
P21 / n , while
In3 ( SO4 )( TeO3 ) 2F3 ( H2O ) formed a non - centrosymmetric ( NCS ) and chiral space group P212121 . -
pubmed
The
CS compound features a 2D layered structure composed of 2D
indium oxide layers decorated by
sulfate tetrahedra and
tellurite groups . -
pubmed
Liver inflammation was assessed by plasma
sCD163 and
sMR levels ( ELISA ) ,
fibrosis by liver stiffness ( transient elastography ) , function by
galactose elimination capacity ( GEC ) , and cognitive performance by continuous reaction time ( CRT ) . -
pubmed
During
DAA - therapy , we observed a rapid
sCD163 decline from baseline to end of treatment ( EOT ) ( 6.9 vs. 3.8 mg / L , p<0.0001 ) , whereas the change in sMR was more subtle ( 0.37 vs. 0.30 mg / L , p<0.0001 ) . -
pubmed
Moreover , we found that the putative cation - binding site of
TMEM180 is conserved among orthologs , and that its position is similar to that of
melibiose transporter
MelB. -
pubmed
BACKGROUND : There is great interest in understanding whether interventions on
sugar - sweetened beverage (
SSB ) consumption through pregnancy and early childhood affect adolescent
body mass index (
BMI ) . -
pubmed
Results : The
PEG - Lipo - beta - E showed good stability and was characterized as 83.31 + - 0.181 nm in size , 0.279 + - 0.004 in polydispersity index (
PDI ) , -21.4 + - 1.06 mV in zeta potential , 6.65 + - 0.02 in pH , 5.024 + - 0.107 mg / mL in
beta - elemene ( beta - E ) content , and 95.53 + - 1.712 % in average EE . -
pubmed
We previously obtained the
AcCHMOM6 mutant , which oxidizes
omeprazole sulfide (
OPS ) to the chiral
sulfoxide drug
esomeprazole . -
pubmed
To further improve the catalytic efficiency of the
AcCHMOM6 mutant , a focused mutagenesis strategy was adopted at the intersections of the
FAD - binding domain ,
NADPH - binding domain and alpha - helical domain based on structural characteristics of
AcCHMO . -
pubmed
AcCHMOM7 ( L55Y / P497S ) was obtained by combining the two mutations , which increased the specific activity from 18.5 ( M6 ) to 108 U / g , and an increase in the K i of the substrate
OPS from 34 to 265 muM. -
pubmed
Many
BDA - labeled axonal fibers and terminals from the OPC were ipsilaterally observed in the rostral and rostroventral regions of the primary somatosensory cortex (
S1 ) , the rostral region of the secondary somatosensory cortex (
S2 ) , and the most rostrocaudal levels of the granular insular cortex ( GI ) . -
pubmed
Furthermore , injections of a retrograde tracer ,
Fluorogold , into these
S1 ,
S2 , and GI regions , resulted in preferential labeling of neurons in the ipsilateral OPC among the intralaminar and sensory thalamic nuclei . -
pubmed
The steady - state levels of intracellular
cyclic AMP were higher than those observed in unstimulated cells , and
cyclic AMP - dependent protein phosphokinase was in a highly activated state as compared to controls . -
pubmed
In contrast , inhibition of growth , apparent cell
death , and unusual morphological changes were observed in both normal and transformed cells when high concentrations of either
PGE1 ( 10 micron ) or the
phosphodiesterase inhibitor
1 - methyl , 3 - isobutylxanthine ( 0.5 mM to 2 mM ) were used , which was indicative of toxic effects of the drugs . -
pubmed
Ribulose diphosphate carboxylase was present in the
methanol -
bicarbonate - grown organism at six times the specific activity of that in the
succinate - grown organism . -
pubmed
Reactivity of two anti -
galactosyl ceramide antibodies towards
myelin basic protein . -
pubmed
Two anti -
galactosyl ceramide antibodies ( polyclonal Ab142 and a monoclonal antibody ) were characterized in terms of their reactivity towards purified lipids and
myelin basic protein . -
pubmed
Binding of
MAb - Gal C to
Gal - C was equally inhibited by
ceramide and
galactose to approximately 50 % , indicating that both groups are important for antibody recognition . -
pubmed
MAb - Gal C was also shown to be reactive with the structurally related
lipids ,
sphingomyelin and
sulfatide . -
pubmed
Polyclonal
Ab142 , although raised against
Gal C , was shown to be 3 - fold more reactive with component 8 ( C-8 ) of
myelin basic protein than
Gal C. On the other hand , the
MAb - Gal C which also reacted with C-8 was 2 - fold less reactive with C-8 than with
Gal C. Neither of these antibodies were reactive with component 1 ( C-1 ) of
myelin basic protein . -
pubmed
An
anti - MBP IgG was shown to be reactive with C-1 and C-8 but unreactive with
Gal C. In competitive inhibition ELISA , C-8 was able to compete out 44 % and 41 % of
Gal C binding to
polyclonal Ab142 and
MAb , respectively . -
pubmed
D - galactose was unable to inhibit C-8 binding to either antibody , whereas
ceramide was as efficient as
Gal C.(ABSTRACT TRUNCATED
AT 250 WORDS ) -
pubmed
Anti -( 14 - 3 - 3 protein ) antibody inhibits stimulation of
noradrenaline (
norepinephrine ) secretion by chromaffin - cell cytosolic proteins . -
pubmed
The
9 trans , 12 cis isomer of linoleic acid is a poor substrate and the
9 cis , 12 trans isomer is a good substrate for the
enzyme that desaturates
dienoic acids at position six . -
pubmed
The therapeutic application of recombinant
NEP protects the airways from the adverse actions of stimuli that release inflammatory peptides , and induction of the
NEP gene expression by glucocorticoids suggest a possible mechanism for the action of these
steroids in treating
airway diseases such as
asthma ,
chronic bronchitis , or
cystic fibrosis . -
pubmed
The method involves the first dimension electrophoresis in
agarose gel containing concanavalin A ( Con A ) or
lentil lectin (
LCH ) , the second dimension immunoelectrophoresis in
agarose gel containing polyclonal antibody against
AFP , reaction with peroxidase - conjugated
Protein A and staining with
4 - methoxy-1 - naphthol . -
pubmed
Binding to Site 1 was characterized by : being displaced by
dextran sulphate or heparin ; being dependent on
Ca2 + ; having a Kd value of about 15 micrograms of protein / ml ; not being significantly displaced by a 20 - fold excess unlabelled
low - density lipoprotein that had been reductively methylated ; being displaced by approx . -
pubmed
As much as 60 % of this
exonuclease activity was similar to that associated with
DNA polymerase delta based upon its sensitivity to inhibition by
GMP and by
butyl - phenyl - deoxyguanosine triphosphate (
BuPdGTP ) . -
pubmed
Association of
3 '---- 5 ' exonuclease activity with the
DNA polymerase in the replitase complex was also demonstrated by analyzing
dTTP turnover to
dTMP in an in vitro
DNA polymerase assay system . -
pubmed
The
DNA polymerase activity in
replitase complex exhibited a sensitivity to
BuPdGTP which both was similar to that of DNA replication in permeable cells and was intermediate between the
BuPdGTP inhibition of purified
DNA polymerases alpha and delta . -
pubmed
A hormone - dependent dissociation of
hsp90 from the cytosolic form of the receptor complex was observed within the first hour of in vivo
progesterone treatment , which could explain the lack of
hsp90 in nuclear receptor complexes . -
pubmed
In a cell - free system ,
hsp90 binding to receptor was stabilized by
molybdate but disrupted by high salt . -
pubmed
The anorectic effect of 4 mg kg-1
quipazine was antagonized by the nonselective
5 - HT - receptor antagonist
methysergide ( 5 mg kg-1 , i.p . ) -
pubmed
and by the selective
5 - HT2 - receptor antagonists
ketanserin ( 1 mg kg-1 and 2.5 mg kg-1 , i.p . ) -
pubmed
The selective
5 - HT3 - receptor antagonist
GR38032F ( 1 mg kg-1 , i.p . ) -
pubmed
, which blocks some of the effects mediated at
5 - HT1 - like receptors , did not block the reduction in food intake produced by this dose of
quipazine . -
pubmed
None of the
5 - HT - receptor antagonists had any effect on food intake when they were administered alone , suggesting that endogenous
5 - HT is not involved in the tonic control of food intake under the conditions of these experiments . -
pubmed
It is concluded that the anorectic action of
quipazine is mediated , at least in part , by activation of
5 - HT2 - receptors . -
pubmed
The
32P incorporation rate into phospholipids ( PC ,
phosphatidylinositol (
PI ) ,
phosphatidylethanolamine (
PE ) ) was affected basicly different analyzing either PC ,
PI or
PE . -
pubmed
Histamine and
propranolol provoked the highest incorporation of
32P ( 240 % increase in
PI labeling ) . -
pubmed
Isoproterenol and
adrenalin decreased PC labeling ( 45 % and 18 % ) whereas
isoproterenol decreased
32P incorporation into
PI ( 18 % ) , and
adrenalin led to an increase ( 37 % ) . -
pubmed
A decrease in
hexadecanoic acid content of
PI was found after administration of either
isoproterenol ,
adrenalin or
histamine . -
pubmed
The
tumor shows tubular structures with
hyaluronic acid , " retinal rosettes " , and areas with
ganglion and
GFAP - positive glial cells . -
pubmed
The detection of
venous thrombosis of the legs using
125 - I - labelled
fibrinogen . -
pubmed
Intravertebral artery injection of
rauwolscine , 5 micrograms enhanced the
BCO response but had no effect on basal blood pressure . -
pubmed
It is concluded that enhancement of the
BCO response by
yohimbine and
rauwolscine is caused by blockade of central
alpha 2 - adrenoceptors . -
pubmed
Platelet density distribution after isopycnic centrifugation on a discontinuous stractan density gradient , dense granule markers (
serotonin ,
ATP and
ADP ) and alpha granule markers ( intraplatelet
beta - thromboglobulin and
platelet factor 4 ) were simultaneously determined . -
pubmed
We have found that the
gamma - carboxyglutamic acid (
GLA)-containing protein from bone (
BGP ,
osteocalcin ) has chemotactic activity in vitro for a number of cells which are found adjacent to endosteal bone surfaces in vivo . -
pubmed
A synthetic peptide (
Phe - Tyr - Gly - Pro - Val ) , which is identical to the
carboxy - terminal peptide cleaved from
BGP when digested by
trypsin , is also chemotactic for the same cells . -
pubmed
BGP retains its chemotactic activity after conversion of the
gamma - carboxyglutamic acid residues to
glutamic acid , indicating that this biological effect requires neither
gamma - carboxyglutamate nor the ability of
BGP to bind
calcium . -
pubmed
Computer analysis of the data for total radioactivity (
PCNU equivalents ) , based upon an open two - compartment model , yielded values of the pharmacokinetic parameters
K12 , K21 , and
K10 of 1.49 hr-1 , 0.25 hr-1 , and 0.19 hr-1 , respectively . -
pubmed
In the groups receiving 8.2 of
omega 3 fatty acids , there was a significant decrease in serum
triglyceride and
very low - density lipoprotein levels , which is in accord with earlier observations . -
pubmed
In the other parameters , including
cholesterol and
high - density lipoprotein cholesterol , no decrease or increase was observed . -
pubmed
Identification of major
tyrosine - phosphorylated proteins in
Csk - deficient cells . -
pubmed
Csk is a non - receptor protein -
tyrosine kinase that acts as a negative regulator of
Src family tyrosine kinases . -
pubmed
In these embryos , an accumulation of
tyrosine phosphorylated proteins was observed as a consequence of constitutive activation of
Src family kinases . -
pubmed
In order to identify those
tyrosine phosphorylated proteins , we established a
Csk - deficient cell line from embryos lacking both
Csk and the anti - oncogene product
p53 . -
pubmed
It also induces in parallel the early
tyrosine phosphorylation of numerous proteins , including
phospholipase C gamma-1 . -
pubmed
A marked conversion of the
T cell - specific tyrosine kinase p56lck to a slow migrating 60 - kDa form is also found following
GM1 addition . -
pubmed
wts of 14,500 and 15,000 , which slightly differ in their isoelectric point ( 4.9 and 5.3 ) and
amino acid composition . -
pubmed
Low
serum albumin and the increased risk of
amikacin nephrotoxicity . -
pubmed
Furthermore , low
serum albumin concentration was also associated with
amikacin accumulation in plasma . -
pubmed
The group of patients with
hypoalbuminemia ( & lt ; or = 3.0 g / dL ) had a significantly higher trough
amikacin plasma level ( assessed at 48 and 96 hours of the initiation of treatment ) than those with normal
serum albumin , with no differences among the age , sex , baseline renal function and received
amikacin doses . -
pubmed
CONCLUSIONS : We conclude that
serum albumin concentration is the most powerful predictor of
amikacin nephrotoxicity . -
pubmed
The data suggest that a structural feature of
FGF3 involving the
amino - terminal region and glycosylation site has a significant bearing on its passage through the Golgi complex and may regulate the secretion of the ligand . -
pubmed
Condensed chromatin can form protruding patches , which eventually bud from the nucleus in sealed vesicles through a process which is
actin - dependent , since it could be blocked by
cytochalasins . -
pubmed
Antigen levels of
tissue plasminogen activator (
t - PA ) ,
urokinase - type plasminogen activator (
u - PA ) ,
plasminogen activator inhibitors type 1 (
PAI-1 ) and
PAI-2 in GCF were determined with ELISAs and
17 beta - oestradiol and
progesterone in serum with radioimmunoassays . -
pubmed
A statistically significant correlation between
delta progesterone and delta
PAI-2 was noted : the higher
delta progesterone , the lower delta
PAI-2 . -
pubmed
Antibody quantitation (
anti - D ) had a positive predictive value of 54.5 per cent and negative predictive value of 85.7 per cent while
MMA had a positive predictive value of 75 per cent and a negative predictive value of 100 per cent . -
pubmed
Methotrexate is partly oxidised by hepatic
aldehyde oxidase to
7 - hydroxymethotrexate . -
pubmed
A gene transcribed from the bidirectional
ATM promoter coding for a
serine rich protein :
amino acid sequence , structure and expression studies . -
pubmed
In an earlier report we showed that the 5 ' end of the gene for
ataxia telangiectasia ATM is within 700 bp of the 5 ' end of a novel gene
E14 , and suggested that the
CpG island that separates these genes functions as a bidirectional promoter . -
pubmed
We have now determined the complete
amino acid sequence of the
E14 protein , defined the exon / intron structure of the gene and estimate that the complete gene is more than 55 kb in length . -
pubmed
The compound under study in serum samples was extracted with Sep - Pak
C18 cartridge and the extract was applied to an
octadecylsilane - bonded column ( Nucleosil 5C18 , 150 x 4 mm I.D. ) -
pubmed
Disturbances in neuronal
calcium homeostasis may result in the activation of several
calcium - sensitive enzymes , including
lipases ,
kinases ,
phosphatases , and
proteases . -
pubmed
One potential pathogenic event in a number of acute CNS insults , including
TBI , is the activation of the calpains ,
calcium - activated intracellular
proteases . -
pubmed
AIM : To investigate separately the effects of
sucralfate on acute
gastric and duodenal injury and on changes in
intragastric bleeding induced by
aspirin . -
pubmed
In this report , using
biotinylated dextran amine (
BDA ) and
cholera toxin subunit B (
CTB ) for anterograde and retrograde labelling , respectively , we investigated projections from the nBOR and LM to the dorsal thalamus . -
pubmed
Two regression models were developed that were based on the age - dependent , relative abundance of 2 cuticular
hydrocarbons ,
pentacosane (
GC peak 1 ) and
nonacosane (
GC peak 5 ) . -
pubmed
Two new regression models were developed that also use the relative abundance of a 3rd cuticular
hydrocarbon ,
octacosane (
GC peak 4 ) . -
pubmed
Furthermore , an inhibitor of the
hypusine modification ,
GC7 (
N ( 1 )- guanyl-1 , 7 - diaminoheptane ) , prevents
CD83 surface expression by apparently interfering with nucleocytoplasmic translocation of the
CD83 mRNA and , importantly , significantly inhibits DC - mediated T lymphocyte activation . -
pubmed
The data presented suggest that
CD83 mRNA is transported from the nucleus to the cytoplasm via a specific nuclear export pathway and that
hypusine formation appears to be essential for the maturation of functional DCs . -
pubmed
ET-1 promotes the apoptosis through an increase in the intracellular
calcium in the CL cells and affects the physiological function of CL cells by inhibiting
progesterone production . -
pubmed
It is concluded that reductions in renal
Cu contents caused by
Cd or LPS administration may be a result of
Cd and
NO displacement of
Cu previously bound to
metallothionein . -
pubmed
Postoperative data I ( first follow - up : n = 66 ):
sinus rhythm ( yes ): 4/7 vs. 47/59 (
NS ) ; pacemaker ( PM ) in AAI mode ( yes ): 1/7 vs. 3/59 (
NS ) ; inducible
atrial fibrillation ( yes ): 2/7 vs. 5/59 (
NS ) ; Bradycardie Tachycardie Syndrome with the need of PM implantation 0/7 vs. 4/59 (
NS ) . -
pubmed
Postoperative data
II ( second follow - up : n = 55 ):
sinus rhythm ( yes ): 5/7 vs. 34/48 (
NS ) ; PM in AAI mode ( yes ): 0/7 vs. 4/48 (
NS ) ; Inducible
atrial fibrillation ( yes ): 2/7 vs. 5/48 (
NS ) ;
Bradycardia Tachycardia Syndrome with the need of PM Implantation 0/7 vs. 5/48 . -
pubmed
All serum samples were analyzed initially by fluorescence polarization immunoassay ( FPIA , TDx ) ( Abbot Diagnostics ; Irving , TX ) and again by
enzyme multiplied immunoassay ( EMIT
Vancomycin Assay ) ( Dade Behring ; San Jose , CA ) . -
pubmed
Mapping the ligand - binding site on the
C5a receptor :
arginine74 of
C5a contacts
aspartate282 of the
C5a receptor . -
pubmed
Mutation of D(282 ) to A decreases the sensitivity of the receptor to activation by intact
C5a but not by its less potent metabolite , C5adR(74 ) , which lacks the C - terminal
arginine(74 ) . -
pubmed
RESULTS :
Propofol inhibited both
IL-6 and
IL-10 production at 0.5 microg / mL , 5 microg / mL and 50 microg / mL. -
pubmed
Conversely ,
thiopental induced
IL-10 production at 2 microg / mL and 20 microg / mL. -
pubmed
CONCLUSION :
Propofol appears to inhibit both
IL-6 and
IL-10 production by LPS - stimulated PBMCs in vitro . -
pubmed
Identification of the functionally important
cysteinyl residue in
pig heart aspartate aminotransferase . -
pubmed
Thalidomide has antiangiogenic and immunomodulatory properties and is an effective inhibitor of
TNF - alpha . -
pubmed
CONCLUSIONS : Detection of
p53 gene mutation with PCR - SSCP -
silver stain combined with cytological examination of sputum samples can improve the diagnosis of
lung cancer . -
pubmed
Preclinical studies provided evidence of preferential conversion of inactive
5 '- DFUR to active
5 - FU in
solid tumours due to the relative overexpression of the final anabolising enzyme ,
thymidine phosphorylase (
TP ) , in neoplastic tissues more than in normal counterparts . -
pubmed
AIM : To establish the differential scanning calorimetric ( DSC ) methodology for controlling the crystal - type B form of
nateglinide . -
pubmed
METHODS : Accurately weighed pure dried (
P2O5 as desiccant for 4 h at 80 degrees C in vacuum ) fine powder of crystal - type B and H of
nateglinide were measured dQ / dT by DSC at heating rate of 10 degrees C. min-1 and temperature between 100 degrees C and 200 degrees C to calculate the enthalpy delta HB and delta
HH . -
pubmed
Pharmacokinetic study of
cisplatin and infusional
etoposide phosphate in advanced
breast cancer with correlation of response to
topoisomerase IIalpha expression . -
pubmed
This study evaluated the combination of
cisplatin with a prolonged infusion of
etoposide phosphate (
EP ) in advanced
breast cancer and correlated response to
topoisomerase II expression . -
pubmed
The
protein solution was mixed with
trichloroacetic acid (
TCA ) ,
perchloric acid (
PCA ) or
guanidine - HCl ( GHA ) , and then exposed in distilled water . -
pubmed
The protein was directly exposed to the solution containing
TCA ,
PCA ,
GHA ,
HCl ,
H ( 2 ) SO ( 4 ) or
acetate buffer (
Post - cast method ) . -
pubmed
All drugs that directly or indirectly increase central
serotonin neurotransmission at postsynaptic
5 - HT ( 1A ) and
5 - HT ( 2A ) receptors can produce
SS . -
pubmed
It is likely that the activation of
5 - HT ( 1A ) receptors by
mirtazapine , the combined
serotonin reuptake inhibition by
venlafaxine and
tramadol , as well as possible
serotonin release by
tramadol , contributed to the development of
SS in this case . -
pubmed
The crystal structure of the title compound ,
C ( 28 ) H ( 26 ) , in the monoclinic space group
C2 / c has a columnar packing arrangement with a 7.20 A axis , a feature common to several
tetraphenylmethanes . -
pubmed
Lansoprazole is extensively metabolized by
CYP2C19 and
CYP3A4 in the liver , whereas
rabeprazole is primarily converted non - enzymatically to
rabeprazole -
thioether , with only some being oxidized by
CYP2C19 and
CYP3A4 . -
pubmed
This article reviews the pharmacokinetic differences between enantiomers of
lansoprazole and
rabeprazole in relation to the
CYP2C19 genotypes . -
pubmed
CYP2C19 influenced the disposition of
( S )- lansoprazole to a greater extent than the ( R)-enantiomer . -
pubmed
The R / S ratios for the AUC of
lansoprazole in
CYP2C19 homEMs ,
hetEMs and
PMs was 12.7 , 8.5 and 5.8 , respectively . -
pubmed
On the other hand ,
( R )- rabeprazole disposition was influenced to a greater degree by
CYP2C19 genetic polymorphisms than
( S )- rabeprazole . -
pubmed
However , the R / S ratios for the AUC of
rabeprazole in
CYP2C19 homEMs ,
hetEMs and PMs was only 1.8 , 2.2 and 2.4 , respectively , suggesting a lesser effect of
CYP2C19 polymorphisms on the stereoselective disposition of
rabeprazole compared to
lansoprazole . -
pubmed
Such a difference in the AUC between
rabeprazole enantiomers is likely to be dependent on stereoselectivity in the
CYP3A4 - mediated metabolic conversion from
rabeprazole -
thioether to
rabeprazole . -
pubmed
We have analyzed the expression of
Fn14 and its ligand
TWEAK in
carotid atherosclerotic plaques and its potential modulation by
atorvastatin in vivo . -
pubmed
Geranylgeranyl transferase inhibitor ,
toxin B (
Rac and
Rho inhibitor ) ,
C3 exoenzyme (
Rho inhibitor ) , and
Y-27632 (
Rho kinase inhibitor ) also decreased
Fn14 expression , implicating the
Rho / Rho kinase pathway in the regulation of
Fn14 expression . -
pubmed
Finally ,
atorvastatin treatment reduced
Fn14 expression in vivo . -
pubmed
Atorvastatin diminishes
Fn14 expression in vitro and in vivo providing novel information of the beneficial properties of statins . -
pubmed
Precise mapping of the putative origin suggested that the replication initiated from a fixed site close to the
AT - rich region and proceeded unidirectionally toward the downstream
rep201 gene , which was further confirmed by
electron microscopic analysis of the ClaI - digested replication intermediates . -
pubmed
OBJECTIVE : To evaluate the effect of
verapamil on the proliferation of normal gingival fibroblast (
NGF ) in vitro . -
pubmed
The 5th passage of
NGF was incubated with 0 , 0.1 , 1 , 10 , 100 micromol / L
verapamil respectively . -
pubmed
An increased whole body
calcium uptake partially compensated dietary
calcium deficiency and was associated with increased gill
chloride cell number in lamellae and filaments in parallel with increased gill
Na (+) K (+)- ATPase activity . -
pubmed
In addition , a single injection of piscine
PTHrP(1 - 34 ) significantly increased whole body
calcium uptake and decreased whole body
calcium efflux . -
pubmed
Administration of
PTHrP significantly increased circulating plasma
calcium 4 - 24 h postinjection . -
pubmed
Genome - wide expression profiles of
pog1Delta and other mtDNA - less cells reveal that many genes involved in response to stimulus , energy derivation by oxidation of organic compounds , cellular
carbohydrate metabolism , and energy reserve metabolism are induced . -
pubmed
DEAD - box proteins catalyze
ATP - driven , local structural changes in RNA or RNA - protein complexes (
RNP ) during which only few RNA base pairs are separated . -
pubmed
The usefulness of the algorithm is demonstrated for the Muller - Brown potential ,
amide hydrolysis , and an 89 atom cluster taken from the active site of
4 - oxalocrotonate tautomerase for the reaction which catalyzes
2 - oxo-4 - hexenedioate to the intermediate
2 - hydroxy-2,4 - hexadienedioate . -
pubmed
OAF was generated and distributed in a bioaerosol test chamber by delivery of
monoterpenes into ozonated air ( 0.1 ppm ) at concentrations of 2.0 ( high ) , 0.75 ( medium ) , or 0.3 ( low ) mgm(-3 ) h(-1 ) . -
pubmed
When exposed to
OAF ( high and medium concentrations in 0.1 ppm
ozone ) there were significant differences after 20 min . -
pubmed
Female astrocytes sustain less cell
death from
oxygen -
glucose deprivation (
OGD ) than male astrocytes . -
pubmed
Arimidex , an
aromatase inhibitor , abolishes these sex differences . -
pubmed
Midazolam was administered at 0.3 mg kg(-1 )
IV as pre - medication at least 1 minute prior to induction . -
pubmed
The
amide I vibrational mode , primarily associated with peptide - bond
carbonyl stretches , has long been used to probe the structures and dynamics of peptides and
proteins by infrared ( IR ) spectroscopy . -
pubmed
MATERIAL AND METHODS : Systemic 3 -
NP ( 10 mg / kg ) administration for 14 days significantly reduced locomotor activity ,
body weight , grip strength , oxidative defense ( raised levels of lipid peroxidation ,
nitrite concentration , depletion of antioxidant enzyme ) , and mitochondrial enzymes activity in striatum , cortex , and hippocampal regions of the brain . -
pubmed
Here , we establish a high - resolution contact map of
RutR carP1 complexes for backbone and base - specific contacts , analyze DNA bending , determine the DNA sequence specificity of
RutR binding by saturation mutagenesis , demonstrate that
uracil but not
thymine is the physiologically relevant ligand that inhibits the DNA binding capacity of
RutR and build a model of the
RutR operator DNA complex based on the crystal structures of
RutR and of the DNA - bound family member
QacR. -
pubmed
Changes in left ventricular mass index ( LVMI ) measured by cardiac ultrasonic indices , HRT parameters , including the original heart rate ( TO ) and slope coeffificient (
TS ) , systolic and
diastolic blood pressures (
SBP and
DBP ) , as well as blood
cholesterol level (
TC ) were measured before and after treatment . -
pubmed
Quartz crystal microbalance - based biosensor for the detection of alpha -
thalassemia 1 (
SEA deletion ) . -
pubmed
Here , a DNA piezoelectric biosensor based on a
quartz crystal microbalance ( QCM ) was created to identify abnormal genes causing
alpha - thalassemia 1 (
SEA deletion ) . -
pubmed
METHODS : The functionalized gold electrode of the
quartz crystal was coated with
avidin and the biotinylated DNA probe was attached . -
pubmed
We have demonstrated that cyclodextrin (
CD ) ( a non - polymeric system ) can be electrospun into nanofibers by itself ; methyl - beta - cyclodextrin (
MbetaCD ) nanofibers were electrospun and it was observed that the success of the electrospinning of the
CD nanofibers strongly depends on ( i ) type of solvent , ( ii ) CD solution concentration and ( iii ) intermolecular interactions between the CD molecules . -
pubmed
There is also an optimism that new approaches , including response prediction based on sequential
18FDG - PET scanning following induction chemotherapy , and novel drugs targeted at
EGF ,
EGFR ,
VEGF , and
tyrosine kinase inhibition may improve treatment pathways and outcomes . -
pubmed
Dioxins exert their toxic actions by acting on phase I and phase
II enzymes , such as
cytochromes P450 ,
superoxide dismutase , and
glutathione peroxidase , promoting cell proliferation , growth arrest , and apoptosis , affecting
cancer homeostasis and neuronal function . -
pubmed
Furthermore , the amplitude of postsynaptic
G protein - coupled inwardly rectifying
K (+ ) channel (
GIRK ) currents elicited by
UK-14304 was significantly larger in the
diabetic group than in the control group . -
pubmed
In addition , the regulation of
budR transcription was elucidated and found to be subject to negative control by
BudR itself and to positive control by external stimuli such as
N -( 3 - oxohexanoyl )- L - homoserine lactone (
OHHL ) quorum sensing signaling molecules and
acetate . -
pubmed
Interestingly , however , we observed that induction of
budR transcription by
OHHL or
acetate did not require
BudR , indicating the involvement of additional regulatory factors in relaying these environmental signals to the
budR promoter . -
pubmed
Zinc chelation with
hydroxamate in
histone deacetylases modulated by water access to the linker binding channel . -
pubmed
Using Born - Oppenheimer ab initio QM / MM MD simulations , a state - of - the - art approach to simulating metallo - enzymes , we have found that the
hydroxamic acid remains to be protonated upon its binding to
HDAC8 , and thus disproved the mechanistic hypothesis that the distinct
zinc -
hydroxamate chelation modes between two
HDAC subclasses come from different protonation states of the
hydroxamic acid . -
pubmed
Instead , our simulations suggest a novel mechanism in which the chelation mode of
hydroxamate with the
zinc ion in
HDACs is modulated by water access to the linker binding channel . -
pubmed
This new insight into the interplay between the linker binding and the
zinc chelation emphasizes its importance and gives guidance regarding linker design for the development of new class - IIa - specific
HDAC inhibitors . -
pubmed
[ Successful treatment of
post circumcision
glanular ischemia -
necrosis with
hyperbaric oxygen and intravenous
pentoxifylline ] . -
pubmed
Univariate and multivariate analyses showed that intravenous
sufentanil significantly reduced
BRF survival rate , hazard ratio 7.78 [ 95 % confidence interval ( CI ) 5.79 , 9.78 ) , for
extracapsular extension stage pT 2 or less , hazard ratio 0.44 ( 95 % CI 0.12 , 0.75 ) , Gleason score at least 7 , hazard ratio 1.96 ( 95 % CI 1.65 , 2.26 ) , positive margin , hazard ratio 1.87 ( 95 % CI 1.58 , 2.02 ) and lymph node involvement , hazard ratio 1.77 ( 95 % CI 1.27 , 2.27 , P & gt ; 0.05 ) . -
pubmed
CONCLUSION : This retrospective analysis suggests that intraoperative
sufentanil administration is associated with an increased risk of
cancer relapse after
RRP , whereas epidural analgesia , with local anaesthetic and opioid , was not associated with a significant effect . -
pubmed
The signalling enzymes
PI3 K ,
TorC2 ,
PLA2 and
sGC are not required for
Ras activation and chemotaxis to
folate or to steep gradients of
cAMP , but they provide a memory of direction and improved orientation of the cell , which together increase the sensitivity about 150 - fold for chemotaxis in shallow
cAMP gradients . -
pubmed
The presence of short - lived radionuclides in the early solar system , especially
( 60 ) Fe , ( 26)
Al , and
( 41 ) Ca , requires a nearby supernova shortly before our solar system was formed , suggesting that the Sun was formed in a massive
star - forming region similar to Orion or Carina . -
pubmed
Because the Sun formed in a region where many other
stars were forming more or less contemporaneously , the bi - polar outflows from all such
stars enriched the local region in interstellar
silicate and
oxide dust . -
pubmed
Surrogate markers of myocardial (
troponin T or I and
CK - MB ) and renal ( serum
creatinine )
injury for primary outcomes were abstracted . -
pubmed
The original
pyrogallol (
1,2,3 - trihydroxybenzene ) method , which was developed specifically for
superoxide dismutase , is now widely used for measuring
superoxide - scavenging of other antioxidants . -
pubmed
Post - digestion
18O exchange / labeling for quantitative shotgun proteomics of membrane proteins . -
pubmed
Post - digestion
( 18 ) O exchange is a simple and efficient method for differential
( 18 ) O /( 16 ) O stable isotope labeling of two biologically distinct specimens , allowing relative quantitation of proteins in complex mixtures when coupled with shotgun
MS - based proteomics . -
pubmed
Whilst this protocol depicts solubilization of detergent - insoluble membrane proteins coupled with
post - digestion
( 18 ) O labeling , it is amenable to any complex membrane
protein mixture . -
pubmed
Described approach relies on solubilization and tryptic digestion of membrane proteins in a buffer containing 60 % ( v / v )
methanol followed by differential
( 18 ) O /( 16 ) O labeling of
protein digests in 20 % ( v / v )
methanol buffer . -
pubmed
The use of
methanol - based buffers in the context of the
post - digestion
( 18 ) O exchange / labeling eliminates the need for detergents or chaotropes that interfere with LC separations and peptide ionization . -
pubmed
, 2012 ) and suggests a possible role for
Chk1 Ser317 phosphorylation as a biological marker for future analyses of complex mixtures of
PAHs . -
pubmed
Prognostic impact of
( 18 ) FDG - PET -
CT findings in clinical stage III and IIB
breast cancer . -
pubmed
RESULTS : With increasing decoction time , the acute
toxicity of detoxicated Fuzi became decreased in the following order :
dBfp-30 ( LD50 of 145.1g / kg ; MTD of 70g / kg ;
MLD of 100g / kg ; NOAEL of 70g / kg ) & gt;dBfp-60 ( too large LD50 ; MTD of 160g / kg ;
MLD of 190g / kg ; NOAEL of 100g / kg ) & gt;dBfp-120 ( no LD50 ; unlimited MTD ; unlimited
MLD ; NOAEL of 130g / kg ) . -
pubmed
Glucose ,
insulin , appetite regulatory hormones ,
GLP-1 ,
GLP-2 , appetite sensations , and markers of
inflammation were measured at a following standardised breakfast , that is at 11 to 14 h
post the evening meals . -
pubmed
TCBQ reductase (
PcpD ) , an
FMN- and
NADH - dependent reductase , catalyzes the reduction of
TCBQ to
tetrachlorohydroquinone . -
pubmed
In the presence of
PcpD ,
TCBQ formed by
pentachlorophenol hydroxylase (
PcpB ) is sequestered until it is reduced to the less toxic
tetrachlorohydroquinone , protecting the bacterium from the toxic effects of
TCBQ and maintaining flux through the pathway . -
pubmed
The toxicity of
TCBQ may have exerted selective pressure to maintain slow turnover of
PcpB ( 0.02 s(-1 ) ) so that a transient interaction between
PcpB and
PcpD can occur before
TCBQ is released from the active site of
PcpB. -
pubmed
Recent progress has begun to reveal the often complex and changing roles of
phosphotyrosine and
phosphoinositide phosphatases in regulation of immunoreceptor signaling . -
pubmed
Quantitative assessment of global
lung inflammation following radiation therapy using
FDG PET /
CT : a pilot study . -
pubmed
The aim of this study was to quantify global
lung inflammation following radiation therapy using
FDG PET /
CT . -
pubmed
Minocycline modulates
cytokine and gene expression profiles in the brain after whole - body exposure to radiation . -
pubmed
Minocycline treatment significantly increased the levels of
interleukin ( IL )- 10 ,
IL-15 and
vascular endothelial growth factor (
VEGF ) in the brain on day 4 in one or more irradiated groups compared to radiation - alone ( p<0.05 ) . -
pubmed
Microarray analysis of brains on day 32 showed that while radiation increased expression of inflammatory genes such as
Il1f10 ,
Il17 ,
Tnfrsf11b ,
Tnfsf12 ,
Il12b and
Il1f8 , these were no longer up - regulated in the
minocycline - treated groups . -
pubmed
Pathway analysis based on gene data suggested that
catenin - beta1 and
tumor suppressor - related transcription regulation were significantly activated by radiation and/or
minocycline ( activation z - score & gt;2.0 ) . -
pubmed
The addition of the outer membrane phospholipase gene
pldA on a multicopy plasmid in a
vpsC or
vacJ mutant restored its resistance to
SDS , suggesting a restoration of lipid asymmetry to the outer membrane . -
pubmed
Increased
PldA levels also failed to restore the mutant 's
phospholipid profile to that of the wild type . -
pubmed
To determine if treatment with
ACTH , a drug widely used to treat early - life
seizures , altered outcome we administered
ACTH once per day subcutaneously during the time of the induced interictal spike activity . -
pubmed
We demonstrate an increase in the abundance of
PHEOPHORBIDE a OXYGENASE (
PAO ) , a key enzyme of
Chl breakdown , together with an accumulation of phyllobilins , that is , products of
PAO - dependent
Chl breakdown , in both species . -
pubmed
The roles of the
PAO / phyllobilin pathway during different plant developmental processes that involve
Chl breakdown , such as leaf senescence and desiccation , fruit ripening and seed maturation , are discussed . -
pubmed
Sulfonamides and their isosters as
carbonic anhydrase inhibitors . -
pubmed
Molecules containing the
sulfonamide group (
R - SO2NH2 ) as well as its structurally related isosters ,
sulfamido (
R - NH - SO2NH2 ) and
sulfamato (
R - O - SO2NH2 ) , constitute the most important class of inhibitors acting on the
metalloenzyme carbonic anhydrase (
EC 4.2.1.1 ) . -
pubmed
The current review is intended to highlight the structural basis of the interactions of
sulfonamide - like groups within the active site of the
carbonic anhydrases and will summarize the clinical use of the most interesting molecules for the treatment of relevant pathologies , such as
glaucoma ,
obesity ,
cancer and CNS - affecting diseases . -
pubmed
They survived and retained dopaminergic function following stereotaxic injection into the
MPTP - lesioned right - side substantia nigra as indicated by SPECT measurement of
DAT activity . -
pubmed
Injected cells preserved and supplemented the remaining endogenous population of
dopamine neurons (
TH - positive cell ipsilateral / contralateral ratio was 56.81 % + - 7.28 % vs. 3.86%+-1.22 % in vehicle - injected controls ; p<0.05 ) . -
pubmed
EKB-569 - treated infected gerbils had significantly decreased abundance of
Cox-2 ,
Adam17 and
Egfr gastric transcripts relative to infected animals on control diet . -
pubmed
Serum total
testosterone , free
testosterone ,
interleukin 1 ( IL ) alpha and beta ,
IL6 ,
tumor necrosis factor ( TNF ) alpha ,
orexin ,
galanin ,
neuropeptide Y ,
tumor necrosis factor - like weak inducer of apoptosis and
tumor necrosis factor receptor - associated factor 6 , and
C - reactive protein (
CRP ) levels were investigated . -
pubmed
In addition to
cachexia , serum
CRP ,
testosterone , and
TNF alpha levels were statistically significantly correlated with OS in refractory
cachexia . -
pubmed
This end - stage term of
cancer duration may be predicted by decreasing serum
testosterone and increasing serum
TNF alpha levels , as well as serum
CRP levels . -
pubmed
Comparison of single intra operative versus an intra operative and two
post operative injections of the
triamcinolone after wedge excision of
keloids of helix . -
pubmed
OBJECTIVE : To compare single intra - operative versus an intra - operative and two
post - operative injections of
triamcinolone after wedge excision of
keloids of helix . -
pubmed
The performance of DFT methodology to predict with accuracy the isotropic hyperfine coupling constants ( hfccs ) of aromatic radicals containing
( 14 ) N nucleus is investigated by an extensive study in which 165 hfccs , belonging to 38 radical species , are obtained from calculations with B3LYP and PBE0 functionals combined with 6 - 31 G * , N07D , TZVP , and
EPR - III basis sets , and are compared to the reported experimental data . -
pubmed
The contribution of
K (+ ) channels ,
sGC /
cGMP pathway ,
phosphodiesterases , and extra and intracellular
Ca ( 2 + ) sources were analyzed . -
pubmed
In the control group ,
TERPY relaxation was induced by the activation of
K (+ ) channels and reduction of the
calcium influx , while in the OVA group , these same effects were also brought about by
TERPY , but with participation of the
sGC /
cGMP pathway . -
pubmed
In both groups ,
SNP - induced relaxation occurred through the activation of
K (+ ) channels ,
sGC /
cGMP pathway and reduction of
calcium influx . -
pubmed
RESULTS : The mean size of the liposomal
DHA was 137 + - 12 nm with a slightly negative charge ; the encapsulation efficiency of
DHA in the liposomes as determined by LC -
MS /
MS ranged from 60 to 80 % ; our formulation is resistant to oxidation and stable over a range of pH ( 1.0 - 7.4 ) at 37 C for a duration of two hours . -
pubmed
It altered proteins involved in cell growth , cell cycle , and apoptosis more effectively than free
DHA in both cell lines ; it up - regulated
p21 and
cleaved PARP , while
P - AKT and
P - S6 were down - regulated . -
pubmed
Lithocholic acid (
LCA ) , a
hepatotoxic and carcinogenic
bile acid , is metabolized to
3 - ketocholanoic acid (
3 - KCA ) by
cytochrome P450 3A (
CYP3A ) . -
pubmed
Chromatographic separation was achieved on a Waters ACQUITY UPLC
C18 column ( 50x2.1 mm , 1.7mum ) with a gradient system consisting of 0.1 % v / v
formic acid in
water ( solvent A ) and 0.1 % v / v
formic acid in
acetonitrile ( solvent B ) . -
pubmed
Enzyme kinetics experiment indicated that
LCA 3 - oxidation followed the Michaelis - Menten model with an apparent Km of 26 + -7muM and Vmax of 303 + -50pmol / min / mg
protein . -
pubmed
This novel UPLC -
MS /
MS method for quantifying
3 - KCA offers a specific , sensitive , and fast approach to determine liver microsomal
LCA 3 - oxidation . -
pubmed
INTRODUCTION :
Bupivacaine is the most commonly used local anaesthetic for spinal anaesthesia (
SA ) . -
pubmed
Transcriptomics and methylomics of
CD4 - positive T cells in
arsenic - exposed women . -
pubmed
We therefore analyzed the transcriptomes ( by RNA sequencing ) and methylomes ( by target - enrichment next - generation sequencing ) of primary
CD4 - positive T cells from matched groups of four women each in the Argentinean Andes , with fivefold differences in urinary
arsenic concentrations ( median concentrations of urinary
arsenic in the lower- and high -
arsenic groups : 65 and 276 mug / l , respectively ) . -
pubmed
Key genes regulating the immune system , such as
tumor necrosis factor alpha and
interferon gamma , as well as genes related to the
NF - kappa - beta complex , were significantly downregulated in the high -
arsenic group . -
pubmed
Differentially methylated regions that were hyper - methylated in the high -
arsenic group showed enrichment for immune - related gene ontologies that constitute the basic functions of
CD4 - positive T cells , such as isotype switching and lymphocyte activation and differentiation . -
pubmed
In conclusion , chronic
arsenic exposure from drinking water was related to changes in the transcriptome and methylome of
CD4 - positive T cells , both genome wide and in specific genes , supporting the hypothesis that
arsenic causes
immunotoxicity by interfering with gene expression and regulation . -
pubmed
By screening subclones from each of the epigenetically heterogeneous transfectant clones , we finally obtained three optimal subclones that expressed
luciferase and EGFP after
5 - aza - dC treatment with high signal - to - noise ratios . -
pubmed
They share homology to
type B glycosyltransferases and
TagB , an enzyme catalyzing
glycerol transfer to an
N - acetylmannosamine residue in
teichoic acid biosynthesis . -
pubmed
Analysis of a cellular pool of
nucleotide - activated
sugars confirmed the presence of the
CMP - activated
nonulosonic acid derivatives , which are most likely serving as substrates for the corresponding
transferase . -
pubmed
Single gene knock - out mutants targeted at either
transferase were analyzed for S - layer O - glycan composition by ESI -
MS , confirming the loss of the
nonulosonic acid derivative . -
pubmed
Ambroxol treatment did not affect expression of
SP - B and
SP - C. -
pubmed
Post carbon removal nitrifying MBBR operation at high loading and exposure to
starvation conditions . -
pubmed
This study investigates the performance of MBBR nitrifying biofilm
post carbon removal at high loading and
starvation conditions . -
pubmed
coli as well as
pepsin ,
bovine serum albumin and
lysozyme were the analytes studied in contact with the polymer nanolayers in
phosphate buffer solution , pH 7.2 . -
pubmed
In contact with
polyaramides , the
proteins of pI & gt ; 4 reversibly formed the 0.2 - 2.5 nm - thick adsorption layers decomposing on further rinsing with the protein - free eluent . -
pubmed
In contact with
PANI , the
proteins formed stable adsorption layers at pH 7.2 , which needed the pH 3.0 to be eluted . -
pubmed
Thus , in a neutral aqueous medium optimal for separation of biopolymers ,
polyaramides , although did not retain DNA , had a weaker affinity to
proteins as compared to
PANI . -
pubmed
Background & amp ; Objective :
Catalase (
CAT ) is an important endogenous antioxidant enzyme that detoxifies
H2O2 into
water and
oxygen , consequently limiting the deleterious effects of
reactive oxygen species . -
pubmed
The products show two - step
dehydration behaviour from thermogravimetric analysis (
TGA ) , and a higher degree of purity and homogeniety than commercial
zinc phosphate pigment . -
pubmed
We report the vibronic structure of the X+ 2A '' ( D0 ) , A+ 2A ' (
D1 ) and B+ 2A '' (
D2 ) electronic states of the 9H
adenine cation , from the adiabatic ionization energy ( AIE ) up to AIE + 1.8 eV. -
pubmed
CYP2C9 and
VKORC1 polymorphisms are essential determinants in the genotype - guided dosing of
warfarin and may distinguish patients who would benefit from switching to direct oral anticoagulants ( DOACs ) . -
pubmed
RESULTS : We found that treatment of all
HNSCC cells with
Gefitinib and
Erlotinib , two Food Drug Administration - approved
EGFR inhibitors , blocked the activity of
Akt /
mammalian target of the rapamycin (
mTOR ) and
extracellular signal - regulated kinase , two crucial downstream effectors of
EGFR , but up - regulated
IKKbeta /
NF - kappaB signalling . -
pubmed
In keeping with these ,
IKKbeta inhibitor
CmpdA synergistically enhanced the efficacy of
EGFR inhibitors to further inhibit in vitro
HNSCC cell growth . -
pubmed
CONCLUSION : Our data demonstrated that co - targeting
EGFR and
IKKbeta with
Gefitinib and
IKKbeta inhibitors could provide a potential novel therapy for
head and neck squamous cell cancer . -
pubmed
Certain anti - polymerization agents such as
colchicine appear to down - regulate most immune cell types , while inducing dendritic cell maturation and increasing
M1 macrophage population . -
pubmed
After 2 h stimuli of 10 - 7 M
dexamethasone (
DX ) , the osteogenic genes ' expressions were detected by real - time
polymerase chain reaction ( RT - PCR ) at Zeitgeber times 0 , 4 , 8 , 12 , 16 , 20 , and 24 . -
pubmed
Induced by
DX , the mRNA expression of
Col-1 ,
OPN , and
IBSP within the loaded / unloaded rPDLCs oscillated as that of the main
clock gene Per-1 . -
pubmed
Stimulation of natural killer cells with a
CD137 - specific antibody enhances
trastuzumab efficacy in xenotransplant models of
breast cancer . -
pubmed
Overcoming the materials integration challenge for complex
oxides on
silicon using virtual substrates opens a path to develop new electronic devices in the More than Moore
era and
silicon integrated quantum computation hardware . -
pubmed
OBJECTIVE : To assess the therapeutic effect of a novel tension band using 3.0 mm cannulated screw combined with a
titanium cable and specific shims comparatively with patellotibial tubercle cerclage in comminuted
fractures of the inferior
pole of the patella . -
pubmed
In this study , experiments were conducted to investigate the mode of occurrence and the leaching behavior of valuable trace metals ( U , V , and
Ga ) from CA and
( NH4 ) 2SO4 - treated CA (
NCA ) , based on the recovery of
aluminum . -
pubmed
On the other side , a higher liquidus temperature , contributing to the dissolutions of
Al3 + and
Ca2 + , could promote the leaching of U from
NCA as well , whereas the water - leaching behaviors of V and
Ga involved a sophisticated trend with temperature > 40C. -
pubmed
RESULTS : Based on the neutral theory of molecular evolution , we propose a methylation - driven model ( MDM ) that allows predicting the changes in frequencies / proportions of the 16
dinucleotides and in the
GC content of a genome given the known number of
CpG to
TpG /
CpA mutations . -
pubmed
The application of MDM to the 10 published vertebrate genomes shows that , for most of the 16
dinucleotides and the
GC content , a good consistency is achieved between the predicted and observed trends of changes in the frequencies and content relative to the assumed initial values , and that the model performs better on the mammalian genomes than it does on the lower - vertebrate genomes . -
pubmed
In the presence of target protein
lysozyme , the interaction of
lysozyme with aptamer led to the dissociation of dsDNA and release of the aptamer -
lysozyme complex to solution , with DNA - c retaining on the electrode ; then , the photosensitizer
phloxine B (
PB ) was assembled on the electrode since GO can simultaneously adsorb DNA - c and
PB molecules . -
pubmed
Upon irradiation by a green LED ,
1O2 was generated by photocatalysis of
PB molecules and then cleaved the DNA - c , leading to remarkably decreased impedance signals that linearly respond with the logarithm of
lysozyme concentration . -
pubmed
Graphical abstract An on - line assembly of photosensitizers and DNA on electrode was developed using
graphene oxide a cartridge and the photocatalytic electrosensor can be used for label - free detection of
lysozyme as low as 1 pM. -
pubmed
Similarly to
Levetiracetam (
LEV ) , BRV works by binding
SV2A vesicles with a high affinity and a linear pharmacokinetic profile . -
pubmed
The
Cd concentrations in the above - ground portion of the plants were significantly decreased as a result of the incorporation of all biochars compared to the unamended soil , with reduction ratios following the order PM ( 78 % ) > >
WS ( 31 % ) RH ( 29 % )
MB ( 28 % ) CY ( 26 % ) > CE ( 19 % ) . -
pubmed
Finally , ubiquitination of
proliferating cell nuclear antigen (
PCNA ) is induced during
CCl4 - induced
liver fibrosis and associated with the DNA damage response ( DDR ) . -
pubmed
While mRNA levels remained unaffected by
AgNO3 or cit - AgNP , the enzyme activity of
GPx was inhibited by
AgNO3 ( 1 microM ) and cit - AgNP ( 5 microM ) and
TrxR activity was inhibited by
AgNO3 ( 0.4 microM ) and
cit - AgNP ( 1 , 5 microM ) . -
pubmed
The comparison of
immunoglobulin levels with
HLA antigens , was less striking , although
HLA - A2 appeared to be associated with low levels of
IgE. -
pubmed
Effect upon serum
LH ,
FSH ,
estradiol and
progesterone . -
pubmed
Interaction of
alloxan and anomers of
D - glucose on
glucose - induced
insulin secretion and biosynthesis in vitro . -
pubmed
Prior exposure to
alloxan alone produced marked inhibition of subsequent
glucose - induced
insulin secretion and biosynthesis . -
pubmed
The anomeric preference of
D - glucose for protecting islet cells from the inhibitory effect of
alloxan on
glucose - induced
insulin secretion and biosynthesis was similar to that for triggering
insulin secretion . -
pubmed
It is suggested that a glucoreceptor , stereospecific to the alpha anomer of
D - glucose , may exist for both
insulin secretion and biosynthesis . -
pubmed
The
HO mRNA level increased to a maximum 15 h after
heme injection . -
pubmed
In contrast , expression of
HO was maximal about 45 h after
heme injection . -
pubmed
Effects of the preferential
dopamine autoreceptor antagonist
( + ) - AJ76 in the intracranial self - stimulation paradigm . -
pubmed
If metastatic
tumor cells have decreased levels of active homotypic cell adhesion
molecules (
CAMs ) , this might aid their escape from the primary
tumor . -
pubmed
Dissection of helix capping in
T4 lysozyme by structural and thermodynamic analysis of six
amino acid substitutions at
Thr 59 . -
pubmed
Threonine 59 , a helix - capping residue at the
amino terminus of the longest helix in T4 phage
lysozyme , was substituted with
valine ,
alanine ,
glycine ,
serine ,
asparagine , and
aspartic acid . -
pubmed
The crystal structures of the different variants showed that changes in conformation occurred at the site of substitution , including
Asp 61 , which is nearby , as well as displacement of a
solvent molecule that is
hydrogen - bonded to the
gamma - oxygen of
Thr 59 in wild - type
lysozyme . -
pubmed
This suggests a structural basis for the heretofore puzzling observations that
asparagine is more frequently observed as a helix - capping residue than
threonine [ Richardson , J. S. , & amp ; Richardson , D. C. ( 1988 ) Science 240 , 1648 - 1652 ] yet
Thr ---- Asn replacements at N - cap positions in
barnase were found to be destabilizing [ Serrano , L. , & amp ; Fersht , A. R. ( 1989 ) Nature 342 , 296 - 299]. -
pubmed
The duration of this
hyperalgesia was prolonged by the
phosphodiesterase inhibitors ,
isobutylmethylxanthine and
rolipram . -
pubmed
Forskolin hyperalgesia was antagonized by the Rp isomer of
cyclic adenosine-3'5 '- monophosphothioate , an analog of
cAMP that prevents the phosphorylation of the
cAMP protein kinase . -
pubmed
Although the
cAMP second messenger system contributes to primary afferent
hyperalgesia , we found no evidence for a contribution of
protein kinase C. -
pubmed
Thus ,
hyperalgesia induced by
prostaglandin E2 ,
prostacyclin (
prostaglandin I2 ) ,
( 8R,15S )- dihydroxyicosa ( 5E-9,11,13Z ) tetraenoic acid , the
adenosine A2 - agonist
2 - phenylaminoadenosine ,
8 - bromocyclic adenosine monophosphate and the direct activator of
adenyl cyclase ,
forskolin , were not significantly attenuated by the selective inhibition of
protein kinase C by the 19 - 31 fragment of
protein kinase C. -
pubmed
Two other inhibitors of
protein kinase C ,
sphingosine and
staurosporine , also failed to attenuate
prostaglandin E2 - induced
hyperalgesia . -
pubmed
The three thrombolytic agents currently available for the management of
AMI are
streptokinase ,
alteplase , and anistreplase . -
pubmed
The
amino acid sequence deduced from its coding base sequence resembles that of
T cell activating protein , most conspicuously in
cysteine residues , 10 out of 11 of which occupy identical positions in an overall sequence homology of 24.8 % . -
pubmed
The assay that has been developed using these modifications and a solid - phase second
antibody separation step , is both sensitive ( with a lower detection limit of 0.5 pg / tube ) , reliable and simple and has the advantage that
methyl oximation of the sample protects the
PGE from degrading prior to and during the assay . -
pubmed
At the end of the 12 - week treatment period , the mean number of pustules ( + /- SEM ) had decreased from 57.8 ( + /- 8.6 ) to 3.9 ( + /- 1.6 ) in the
acitretin group and from 57.1 ( + /-
14.1 ) to 5.7 ( + /- 2.7 ) in the
etretinate group . -
pubmed
Substance P mimics the increase in vascular permeability caused by vagal nerve stimulation and
neurokinin A mimics noncholinergic bronchoconstriction by vagal nerve stimulation . -
pubmed
Incubation with micromolar levels of
ATP dissociates this complex in vitro into
p53 and
hsc70 110 - kDa components . -
pubmed
Incubation of the immunopurified
p53 -
dnaK complex with
ATP resulted in the dissociation of the
p53 -
dnaK complex as it did with the
p53 -
hsc70 complex . -
pubmed
Neither inhibition of
ethanol metabolism with
4 - methylpyrazole , nor a 10 - fold elevation of
acetaldehyde levels by inhibition of
aldehyde dehydrogenase with
cyanamide or
disulfiram , affected the magnitude of the
GSH depletion observed in vivo . -
pubmed
This finding suggests that
antigen presentation by mature M phi is a reversible function regulated by
T cell factors . -
pubmed
The fractionation of
arginine - rich
histones from fetal calf thymus by exclusion chromatography . -
pubmed
Addition of
urate to anaerobic neutrophils causes the reduction of a
b - type cytochrome , a likely component of the neutrophil
superoxide generating system . -
pubmed
Bone resorption as measured by increases in the marrow ( inner ) diameter of the midshaft of the femur and humerus and by the weekly losses of total
[ ( 3 ) H ] tetracycline and
[ ( 3 ) H]
collagen per whole bone was not significantly different among any of the groups studied . -
pubmed
Hill coefficients and IC50 values derived from the inhibition of specific
[ 3H ] L - quinuclidinyl benzilate receptor binding suggest the presence of two
muscarinic binding sites , differing both in affinity for
pirenzepine and in tissue distribution . -
pubmed
Special equipment for the reaction was made with a uv - irradiation lamp and a reaction
coil of
Teflon tubing inside a temperature - controlled reaction box . -
pubmed
When
18 - hydroxy-11 - deoxycorticosterone was incubated with adrenocortical mitochondria fortified by adding exogenously
adrenodoxin reductase and
adrenodoxin in the presence of an
NADPH - generating system , the
steroid was converted to two products which were separated by high performance liquid chromatography . -
pubmed
Production of the unidentified substance was also shown when
18 - hydroxy-11 - deoxycorticosterone was incubated with purified
cytochrome P-45011 beta and its electron transport system . -
pubmed
The enzyme system effecting this conversion is an
NADPH - requiring microsomal
oxygenase found in the liver . -
pubmed
Thus , these two alkaloids appear to be new
sodium channel neurotoxins acting through different mechanisms ,
dibromosceptrin modifying the channel inactivation characteristics and
clathrodin probably influencing channel ionic conductance . -
pubmed
Differential modulation by
cyclothiazide and concanavalin A of desensitization at native
alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionic acid- and
kainate - preferring
glutamate receptors . -
pubmed
Concanavalin A ,
cyclothiazide , and
aniracetam , ligands that modulate desensitization at
glutamate receptors , were tested for their actions on responses at
kainate - preferring receptors in dorsal root
ganglion ( DRG ) neurons and at
alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionic acid (
AMPA)-preferring receptors in hippocampal neurons . -
pubmed
However , in hippocampal neurons concanavalin A produced only weak potentiation of responses to
kainate and
5 - chlorowillardiine , and after treatment with
lectin responses to
5 - chlorowillardiine remained strongly desensitizing . -
pubmed
The different sensitivities of native
AMPA- and
kainate - preferring
glutamate receptors to
cyclothiazide and concanavalin A should prove useful for the differentiation of
glutamate receptor subtypes in other areas of the central nervous system . -
pubmed
In order to achieve isolation of
endo - alpha - D - mannosidase , a Golgi - located processing enzyme that accomplishes deglucosylation of
glycoproteins with N - linked
carbohydrate units by cleaving the linkage between the
glucose - substituted
mannose residue and the remainder of the
oligosaccharide , we have prepared an affinity matrix ( Glc alpha 1 - ->
3Man - O -( CH2 ) 8CONH - Affi - Gel 102 ) containing the derivative of the characteristic
disaccharide product of this enzyme . -
pubmed
This purified
enzyme was free of other processing
glycosidases and was completely inhibited by
Glc alpha 1 - ->
3 ( 1 - deoxy ) mannojirimycin . -
pubmed
Examination of the
endomannosidase by
SDS - polyacrylamide gel electrophoresis revealed a doublet ( M(r ) 60,000 and 56,000 ) with the bands being of approximately equal density . -
pubmed
Degradation of
c - fos protein (
c - FOS ) in the cytoplasm is very rapid in vivo and constitutes a crucial regulation of the nuclear steady - state level through the control of the amount of full - length
molecules available for nuclear transport . -
pubmed
Using cytoplasmic extracts from various origins , we report herein that
c - FOS degradation can be initiated in a
calcium - dependent manner which involves
cysteine proteases called milli- and micro - calpain . -
pubmed
K1 ( Glu-107 - ->
Lys ) point mutants of
SVLT , which are unable to bind to the
Rb protein family or induce neoplastic transformation , are defective for blocking differentiation in the case of
SVLT1 - 121 but retain the ability to block differentiation in the case of full - length
SVLT . -
pubmed
Blood was taken at
rest and 0 and 2 min after exercise for measurement of
lactate and
ammonia . -
pubmed
Resting
ammonia ( mumol / l ) was higher in NYHA III ( 65.7 ( 6.0 ) ) than in NYHA
II ( 48.0 ( 3.7 ) , P = 0.016 ) ; no difference was found between controls ( 48.0 ( 7.1 ) ) and patients . -
pubmed
The overall
lactate and
ammonia response to exercise was greater in NYHA III than in NYHA
II and controls ( P & lt ; 0.05 ) . -
pubmed
At volitional exhaustion , peak
lactate ( mmol / l : NYHA III 3.31 ( 0.26 ) ; NYHA
II 2.56 ( 0.16 ) ; controls 2.71 ( 0.22 ) ; P = 0.022 NYHA III v NYHA
II ) and
ammonia ( mumol / l : NYHA III ) 126.4 ( 8.97 ) ; NYHA
II 92.9 ( 7.23 ) ; controls 109 ( 16.3 ) ; P = 0.006 NYHA III v NYHA
II ) were higher in severe
congestive heart failure . -
pubmed
Ribonucleotide reductase (
RNR ) catalyzes the rate limiting step in the de novo synthesis of
deoxyribonucleotides by directly reducing
ribonucleotides to the corresponding
deoxyribonucleotides . -
pubmed
To keep balanced pools of
deoxyribonucleotides , all nonviral
RNRs studied so far are allosterically regulated . -
pubmed
The
amino acid sequence identities with the
mouse R1 and
R2 subunits are 58 % and 63 % , respectively . -
pubmed
The
R2 protein contains an
iron -
tyrosyl free radical center verified by
EPR spectroscopy and
iron analyses . -
pubmed
Measurement of
cytidine 5 '- diphosphate reduction by the trypanosome
RNR in the presence of various allosteric effectors showed that the activity is highest with
dTTP ,
dGTP , or
dATP and considerably lower with
ATP . -
pubmed
The presentation of
P12 - 25 is sensitive to
cytochalasin B and D ,
brefeldin A and gelonin , a ribosome - inactivating protein synthesis inhibitor , but less sensitive or resistant to
lactacystin , a highly specific inhibitor of the proteasome . -
pubmed
Interestingly ,
CA-074 , a selective inhibitor of
cathepsin B , inhibited presentation of the polypeptide , indicating its involvement in the degradation of the P12 - 25 polypeptide . -
pubmed
Amino acid residues 420 - 434 of the first
disulfide loop of the sex hormone binding globulinlike domain of
protein S are involved in the interaction of
protein S with
C4BP . -
pubmed
These results suggest that
Lys-423 ,
Gln-427 and
Lys-429 of
protein S are important for normal binding to
C4BP . -
pubmed
This suggests that both in the peptide
PSP-420 and in
protein S ,
Lys-423 and
Lys-429 significantly contribute to binding to
antibody LJ-56 . -
pubmed
When peptides
PSP 420 and SL-6 ( residues 447 - 460 ) with carboxyterminal
amide or
carboxylate moieties were compared to their ability to inhibit
C4BP -
protein S complexation ,
PSP-420 - amide was the most potent . -
pubmed
Among them , the bridged
sulfactams ( X =
OSO3H ) were found to be effective
beta - lactamase inhibitors . -
pubmed
External guide sequences ( EGSs ) are short
oligoribonucleotides , which are designed to bind to a given RNA target and form a precursor
tRNA - like complex . -
pubmed
COUP - TF factors , which are able to dimerize with the
RXRs and to compete with the
retinoid receptors for their DNA binding sites , are suspected to modulate the
retinoid signal transduction pathway . -
pubmed
The aim of this work is to assess whether
RA - induced modifications in the expression of
Krox-20 and Hox genes correlate with alterations of the expression of COUP -
TF genes . -
pubmed
In addition to spatial modifications in the expression patterns of
Krox-20 and
Hox genes , we report here an upregulation of the expression level of
COUP - TFI after
RA exposure . -
pubmed
These data suggest an involvement of
COUP - TFI in the generation of
RA - induced abnormalities , but do not support the hypothesis of an involvement of this factor in the regulation of the expression of
Hox or
Krox-20 genes . -
pubmed
The administration of
GBL increased CRE- and
AP-1 DNA - binding activities in the cerebral cortex and thalamus , but not in other regions such as the hippocampus , cerebellum or pons + medulla oblongata , at doses which induced
absence seizures . -
pubmed
Not only the absence -
seizure behavior but also the increased CRE- and
AP-1 DNA - binding activities in the thalamocortical regions were reversibly inhibited by
ethosuximide , a typical anti - absence drug , and the GABAB antagonists
CGP 35348 and
CGP 46381 . -
pubmed
A gel - supershift assay revealed that the
GBL - induced CRE - binding activity was supershifted by an
anti - CRE - binding protein (
CREB ) antibody , and that
AP-1 DNA - binding activity was blocked by anti -
c - Jun and anti -
c - Fos antibodies . -
pubmed
These results suggest that increased CRE- and
AP-1 DNA - binding activities in the cerebral cortex and thalamus are related to the pathogenesis of generalized
absence seizures and that these increases in DNA - binding activity are related to
ethosuximide- and
GABAB antagonist - sensitive abnormal neuronal activity in the thalamocortical circuit . -
pubmed
Time - resolved
EPR of spin - polarized mobile H atoms in amorphous
silica : The involvement of small polarons . -
pubmed
OBJECT : The possible role of the
polyamine interconversion pathway on
edema formation ,
traumatic injury volume , and tissue
polyamine levels after
traumatic brain injury (
TBI ) was studied using an inhibitor of the interconversion pathway enzyme ,
polyamine oxidase . -
pubmed
CONCLUSIONS : These results demonstrate , for the first time , that the
polyamine interconversion pathway has an important role in the increase of
putrescine levels after
TBI and that the
polyamine oxidase inhibitors , blockers of the interconversion pathway , can be neuroprotective against
edema formation and necrotic cavitation after
TBI . -
pubmed
Three days following MCA occlusion ,
terminal deoxynucleotidyltransferase - mediated
dUTP -
biotin nick end labeling ( TUNEL staining ) revealed a distinct pattern of nuclear staining in many neurons around the ischemic core . -
pubmed
The levels of
calcium ,
phosphorus and
alkaline phosphatase in their sera , as well as their feed intake and
body weight , were evaluated weekly . -
pubmed
The parallel increase of both marker molecules indicates increased systemic
NO production during
GH treatment . -
pubmed
All kidneys were randomized to receive 3.0 mM supplemental
GSH to perfusate or no supplementation ( control ) and were preserved by either cold storage (
CS ) in UW or machine perfused ( MP ) in UW - machine perfusate solution ( MPS ) . -
pubmed
An inhibitor of
Janus kinase 3:4-(4 - hydroxyphenylamino)-6 ,
7 - dimethoxyquinazolin-1 - ium chloride methanol solvate . -
pubmed
CH ( 4 ) O ( WHI - P131 , an inhibitor of
Janus kinase 3 ) , contains four
hydrogen bonds . -
pubmed
Cleavage of
SNAP-25 by
botulinum toxin type A requires receptor - mediated endocytosis , pH - dependent translocation , and
zinc . -
pubmed
Methylamine hydrochloride , an agent that prevents pH - dependent translocation , also antagonized toxin - induced
paralysis and
SNAP-25 cleavage . -
pubmed
Furthermore ,
zinc chelation antagonized toxin - induced
paralysis and
SNAP-25 cleavage . -
pubmed
These results demonstrate that cleavage of
SNAP-25 by botulinum serotype A fulfills the requirements of the multistep model of
botulinum toxin action that includes receptor - mediated endocytosis , pH - dependent translocation , and
zinc - dependent proteolysis . -
pubmed
The described effects are more pronounced in the E194Q mutant than in the E204Q mutant demonstrating different roles of these two
glutamates /
glutamic acids at least in the final stages of the catalytic cycle of
bacteriorhodopsin . -
pubmed
The cloning of the affected genes has now provided novel insights into the role and regulation of signaling by
BMP ,
Nodal ,
Wnt ,
FGF ,
Hedgehog ,
Delta ,
Slit ,
retinoic acid and
lipids . -
pubmed
Inductively coupled plasma mass spectrometry ( ICP /
MS ) with direct injection nebulization ( DIN ) was used to evaluate novel impinger solution compositions capable of capturing elemental
mercury (
Hg0 ) in EPA Method 5 type sampling . -
pubmed
An
iodine based impinger solution proved to be very efficient for
Hg0 capture and was amenable to direct analysis by DIN - ICP /
MS . -
pubmed
Using DIN - ICP /
MS , it was demonstrated for the first time that
iodine can be generated just prior to impinger sampling for efficiently oxidizing
Hg0 and retaining it in solution as
HgI4 ( 2 - ) . -
pubmed
Due to the increased interest in
Hg speciation from combustion sources and the potential for using DIN - ICP /
MS for multiple metals analyses , an impinger sampling train for gaseous
Hg speciation and multiple metals analyses using DIN - ICP /
MS analyses is presented . -
pubmed
The DIN - ICP /
MS instrumental detection limit was 0.003 ppb , and MDLs ranged from 0.007 to 0.116 microg / L ( ppb ) in a variety of impinger solutions used for
Hg capture . -
pubmed
Substitution of
Phe by
leucine in a
ZFY peptide permits
Zn ( 2 +)-dependent folding . -
pubmed
In the present study , we examined which features of the
flavonoid structures were associated with induction of
UGT1A1 and whether common drug - metabolizing enzyme inducers also produce this induction . -
pubmed
We also determined whether
flavonoid treatment affected
sulfate conjugation and
CYP1A1 activity . -
pubmed
In contrast , all tested
5,7 - dihydroxyflavones and -
flavonols induced
CYP1A1 activity (
ethoxyresorufin deethylation ) . -
pubmed
Together , these results strongly suggest that the
flavonoid induction of
UGT1A1 is through a novel
nonaryl hydrocarbon receptor - mediated mechanism . -
pubmed
Therefore , we can conclude that long - lived
DA receptor sensitization , produced by repeated
DA D2 agonist treatments in ontogeny , is associated with enhanced
AMPH - induced DA release in the neostriatum in adulthood , but is not accompanied by evident alteration in
quinpirole - induced DA release . -
pubmed
Nitric oxide ,
vasoactive intestinal polypeptide , and/or
neuropeptide Y are co - localised with
cholinesterase and/or
acetylcholine transporter in some of the nerve fibres supplying the prostate . -
pubmed
A dense network of
cholinesterase - staining fibres supplies both prostate epithelium and stroma , suggesting a role of
acetylcholine and/or co - localised neuropeptides in the modulation of prostatic secretions , as well as smooth muscle tone . -
pubmed
A predominantly epithelial location for
prostate muscarinic receptors indicated a major secretomotor role for
acetylcholine . -
pubmed
He underwent total gastrectomy with splenectomy , as well as
D3 dissection , and received postoperative chemotherapy combining oral
uracil and
futrafur (
UFT ) with
cisplatin (
CDDP ) , but results showed
recurrence of multiple abdominal lymph node
metastases around the aorta . -
pubmed
Electrophysiological ( resting membrane potential , V(m ) ) and biochemical (
neuron - specific enolase activity [ NSE ] ) measurements were taken every 2 d for a period of 16 d. A voltage - sensitive
oxonol dye together with flow cytometry was used to measure relative changes in V(m ) . -
pubmed
Neuron - specific
enolase activity was measured by following the production of
phosphoenolpyruvate from
2 - phospho - d - glycerate at 240 nm . -
pubmed
The
HFE mutation is common and , when homozygous , can lead to a morbid accumulation of body
iron and the disease
hereditary hemochromatosis . -
pubmed
Dietary
iron content was hypothesized to interact with the
HFE genotype to influence oxidative damage in mammary and colon tissue . -
pubmed
Neuroprotective effect of
ACTH ( 4 - 9 ) in degeneration of hippocampal nerve cells caused by
dexamethasone : morphological , immunocytochemical and ultrastructural studies . -
pubmed
The purpose of this research was an assessment of the neuroprotective effect of
ACTH ( 4 - 9 ) in degenerative changes of hippocampal neurons induced by synthetic
GC -
dexamethasone . -
pubmed
It was shown that
dexamethasone administered in toxic doses evokes neuronal
death in layer
CA3 of the hippocampus . -
pubmed
Dexamethasone - induced damage to hippocampal pyramidal neurons ( assessed by cell counts , immunocytochemical analysis of cytoskeletal alterations and ultrastructural studies ) was significantly reduced in animals administered
ACTH ( 4 - 9 ) . -
pubmed
Deubiquitination of
type 2 iodothyronine deiodinase by
von Hippel - Lindau protein - interacting deubiquitinating enzymes regulates
thyroid hormone activation . -
pubmed
The
type 2 iodothyronine deiodinase (
D2 ) is an integral membrane ER - resident selenoenzyme that activates the pro - hormone
thyroxine ( T4 ) and supplies most of the
3,5,3 '- triiodothyronine ( T3 ) that is essential for brain development . -
pubmed
VDU1 , but not
VDU2 , is markedly increased in brown adipocytes by
norepinephrine or cold exposure , further amplifying the increase in
D2 activity that results from
catecholamine - stimulated de novo synthesis . -
pubmed
The present results show that the blockade of
adenosine A2A receptors potentiates the antiparkinsonian effect of
L - DOPA . -
pubmed
OBJECTIVES : To test the sensitivities of recently published American recommendations predicting occult
intracranial lesion (
OICL ) in girls with
central precocious puberty (
CPP ) , and to validate a previously derived diagnosis rule predicting
OICL based on age at puberty onset and
estradiol (
E2 ) level . -
pubmed
RESULTS : Ischemic storage per se did not provoke any changes of the surfactant system ; however , severe alterations occurred within 6 hours of reperfusion , resulting in a severe loss of surface activity , including a decrease in the percentage of the large surfactant aggregate fraction , reduction of the
surfactant apoproteins SP - B and
SP - C , the
dipalmitoyl molecular species of
phosphatidylcholine and
phosphatidylglycerol within the large surfactant aggregate fraction . -
pubmed
Calpains are
calcium - dependent
cysteine proteases and have been implicated in
post - ischemic cell
death . -
pubmed
Whereas
leupeptin , CAI-1 or
Ac - DEVD - CHO did not modify
post - ischemic function , they significantly reduced
infarct size and cardiomyocyte positive TUNEL staining . -
pubmed
A voltammetric study of direct electron transfer to
cytochrome c using a very large assembly of
carbon microelectrodes . -
pubmed
A very large assembly of more than 8000
carbon fibre microdisk electrodes was used to study direct electron transfer to
cytochrome c. -
pubmed
Recently described in this context was the
dUTPase , which may confer resistance to
fluoropyrimidine drugs such as
5 - fluorouracil (
5 - FU ) . -
pubmed
At 28 d of storage , there was a linear ( P & lt ; 0.01 ) increase in
Na , K , and
Mg content in egg yolks as dietary
CLA increased . -
pubmed
On d 28 , there was a linear decrease ( P & lt ; 0.01 ) in the
Na content of albumen with increasing
CLA . -
pubmed
Tamoxifen is a well - known antiestrogen used for the hormonotherapy of
estrogen receptor positive
breast cancer . -
pubmed
In addition to its high affinity binding to the
estrogen receptor (
ER ) ,
tamoxifen binds with comparable affinity to the microsomal antiestrogen binding site ( AEBS ) , and inhibits with a micromolar efficiency ,
protein kinase C (
PKC ) ,
calmodulin (
CaM)-dependent enzymes and
Acyl CoenzymeA : Cholesterol Acyl Transferase (
ACAT ) . -
pubmed
These data provide a useful guide for the conception of selective ligands of
ERs ,
AEBS ,
PKC ,
CaM or
ACAT based on the chemical structure of
tamoxifen . -
pubmed
The relative activity factor approach assumed that
NE-098 formation is predominantly catalysed by
CYP3A4 and
CYP2C9 and the
NE-152 + 163mix ( a mixture of two hydroxylated metabolites ,
NE-152 and
NE-163 ) formation is only catalysed by
CYP3A4 . -
pubmed
The intrinsic clearance of
NE-100 predicted from
rCYPs ( the predicted CLint -
HLM - total ) corresponded to those observed from EM- and PM -
HLM ( the observed CLint -
HLM ) . -
pubmed
The in vivo oral clearance ( CLoral ) of
NE-100 in extensive metabolizers and poor metabolizers of
CYP2D6 was predicted to be 50times higher in extensive metabolizers than poor metabolizers using in vitro - in vivo scaling method based on the dispersion model . -
pubmed
These data suggest that polymorphism of
CYP2D6 might greatly affect
NE-100 metabolism in vivo . -
pubmed
PURPOSE :
14 - 3 - 3sigma is an intracellular , dimeric ,
phosphoserine binding protein that is expressed in epithelial cells and involved in
cancer development . -
pubmed
Here , we describe a new
AR variant found in a hormone - refractory metastatic
PCa , in which
threonine 575 in the DNA binding domain , and threonine 877 in the ligand - binding domain , were both replaced by an alanine . -
pubmed
In vitro effect of the potent
poly ( ADP - ribose ) polymerase (
PARP ) inhibitor
INO-1001 alone and in combination with
aspirin ,
eptifibatide ,
tirofiban ,
enoxaparin or
alteplase on haemostatic parameters . -
pubmed
Since the pathophysiology of acute
ischemic cardiac diseases involves
haemostatic impairment and the therapeutic regimen includes antithrombotic drugs , we investigated the effect of the potent
poly ( ADP - ribose ) polymerase (
PARP ) inhibitor
INO-1001 alone and in combination with platelet aggregation inhibitors (
aspirin ,
eptifibatide and
tirofiban ) , unfractionated
heparin ,
low molecular weight heparin (
enoxaparin ) or the recombinant fibrinolytic drug (
alteplase ) , on various haemostatic parameters in vitro . -
pubmed
Activated partial
thromboplastin time ,
Anti - Xa activity and
euglobulin lysis time were determined in the presence or absence of different concentrations of
INO-1001 , in combination with
sodium heparin ,
enoxaparin or
alteplase , respectively . -
pubmed
Consequently , the
PARP inhibitor
INO-1001 can be safely applied together with the drugs tested . -
pubmed
Immunohistochemistry for
PCNA ,
phospho -
IkappaBalpha , and
phospho -
Erk was also performed . -
pubmed
OBJECTIVE : To determine whether
clomiphene citrate (
CC ) inhibited
gonadotropin - induced ovulation by reducing
cyclic adenosine 3 ' , 5 '- cyclic monophosphate (
cAMP ) and
prostaglandin E2 (
PGE2 ) levels in ovary . -
pubmed
If so , to determine whether
E2 coadministration could protect against these effects of
CC . -
pubmed
MAIN OUTCOME MEASURE(S ): Number of superovulated COCs , ovary and uterus weight ,
FSH and
LH levels in serum , and
cAMP and
PGE2 levels in ovary . -
pubmed
RESULT(S ): The superovulatory dose of
gonadotropins significantly increased ovary and uterus weights ,
cAMP and
PGE2 levels in ovary , and serum levels of
FSH and
LH . -
pubmed
Coadministration of CC ( 10 mg / kg
body weight ) significantly reduced levels of
cAMP ,
PGE2 in the ovary , ovary and uterus weights , and ovulation rate , whereas
FSH and
LH levels were not significantly altered . -
pubmed
In its center , the chromophore , formed from the tripeptide
Gln ( 63 )- Tyr ( 64 )- Gly(65 ) , is tightly held by multiple
hydrogen bonds in a polar cage that is structurally quite dissimilar to that of
avGFP . -
pubmed
In this work we have searched for experimental evidence that would corroborate the idea that
nodal threshold (
NT ) is the minimum electric field strength able to promote direct
sinus node reset that , in turn , can be verified by a decrease in corrected
sinus node recovery time ( CSNRT ) . -
pubmed
Immobilization of
uricase within
polystyrene using
polymaleimidostyrene as a stabilizer and its application to
uric acid sensor . -
pubmed
A novel amperometric
uric acid ( UA ) sensor has been developed by coating the surface of a gold electrode with a
polystyrene ( PS ) membrane formed by 30 microL of a 30 mg mL(-1 ) PS
chloroform solution combined with 30 microL of a 5 mg mL(-1 )
polymaleimidostyrene (
PMS ) solution as a dispersant for enzyme ,
uricase ; this membrane has been successfully employed as an immobilization support for
uricase . -
pubmed
In the PS membrane ,
PMS forms micelle - like structures containing
uricase in an active state . -
pubmed
This immobilized
uricase membrane permits the permeation of
oxygen , which is consumed by the
uricase reaction . -
pubmed
To point out the analytical methodology the
dehydration process of
l - malic acid , in the presence of
Fumarase enzyme , has been used . -
pubmed
At the same time hepatic function and blood fat indexes including
AST ,
ALT ,
Alb ,
Chol and
LDL - C were measured also . -
pubmed
On the other hand , LRAE showed some effects in improving the liver function and reducing blood lipids by decreasing the levels of
AST ,
ALT ,
Chol and
LDL - C. All these changes had a significant dose - effect and time - effect relationship . -
pubmed
Much of the immune function of
S1P results from the engagement of a family of
G - protein - coupled receptors (
S1PR1 -
S1PR5 ) . -
pubmed
Recent findings on the role of
S1P in immunosurveillance , the discovery of regulatory mechanisms in
S1P - mediated immune - cell trafficking and new advances in understanding the mechanism by which
S1P affects immune - cell function indicate that the alliance between
S1P and its receptors has a fundamental role in immunity . -
pubmed
BACKGROUND & amp ; OBJECTIVE : The present investigation was undertaken to study the
iodine nutritional status of school children of Imphal east district in Manipur where endemic
goitre persists during
post - salt iodization phase along with the investigation of the factors responsible for the occurrence of
goitre endemicity . -
pubmed
Thus , the existing dietary supplies of
thiocyanate in relation to
iodine may be a possible aetiological factor for the persistence of endemic
goitre in the study region during
post salt iodization period . -
pubmed
Significant inhibition of viral adsorption onto red blood cells with minimum inhibitory concentrations ranging from 5.3 to 16.8 mM suggested that
AL-1 was capable of directly interfering with viral
hemagglutinin to block binding to cellular receptors . -
pubmed
ADAR1 (
adenosine deaminase acting on RNA ) catalyzes the conversion of
adenosine to
inosine , a process known as A - to - I editing . -
pubmed
Phospho -
Stat3 expression was examined by Western blotting and immunofluorescence analysis . -
pubmed
PURPOSE : Dynamic contrast - enhanced magnetic resonance imaging ( DCE - MRI ) measured the early vascular changes after administration of
TRA-8 ,
bevacizumab , or
TRA-8 combined with
bevacizumab in
breast tumor xenografts . -
pubmed
CONCLUSION : DCE - MRI detected significantly lower K ( trans ) levels in
breast tumor xenografts following
TRA-8 monotherapy or combined therapy with
bevacizumab . -
pubmed
In our previous work ,
hydrogen peroxide is a product of
methanol detoxification through
alcohol oxidase and
NADPH oxidase activation , and acts as a secondary messenger for the activation of
ascorbate - related genes . -
pubmed
Based on our study , transfusion of
ER RBCs before completion of routine blood bank testing carries a low risk of non - ABO alloantibody - mediated
HTRs ( 1/265 [ 0.4 % ER episodes ] ) and receipt of
antigen - incompatible RBCs ( 7/265 [ 2.6 %
ER episodes ] ) . -
pubmed
Flash pyrolysis
GC -
MS was used to examine
nitrogen and
phenol containing compounds ( M.W.=35 to 450 g / mol ) in the particulate fraction of the milk condensate , combined clean wastewater and aerobic bioreactor effluent . -
pubmed
Compounds detected by pyrolysis
GC -
MS were found mostly in the bioreactor with the
amino acid arginine ( 220 mg / kg ) and the
amino acid derivative
1 - methyl-5 - oxo - L - proline methyl ester ( 130 mg / kg ) found at the highest concentrations . -
pubmed
In comparison ,
sterols detected in the effluent were found at higher concentrations when using solvent extraction indicating some degradation with pyrolysis
GC -
MS . -
pubmed
METHODS : Fasting plasma
glucose ( FPG ) , oral
glucose tolerance test ( OGTT ) overload 2 h
glucose ( 2hPG ) , glycosylated
hemoglobin A1c (
HbA1c ) and fasting
insulin ( FIns ) level were measured , respectively . -
pubmed
This mutation induced lower protein yield [ ( 12.7 + -1.72 ) mg / L vs (
16.2 + -2.65 ) mg / L , P<0.01 ] , lower appetency for
glucose [ S0.5 : ( 13.96 + -1.89 ) mmol / L vs ( 5.92 + -0.99 ) mmol / L , P<0.001 ] and
ATP [ Km:(3.27 + -1.14 ) mmol / L vs ( 0.30 + -0.09 ) mmol / L , P<0.001 ] , lower catalytic ability [ Kcat : ( 1.62 + -0.35)/s vs ( 25.18 + -2.10)/s , P<0.001 ] . -
pubmed
Most classical AEDs (
PB ,
PHT ,
CBZ , and SV ) have some untoward effect on cognition , particularly ,
PB . -
pubmed
We found a G2203A
EGFR mutation , resulting in a G735S
amino acid change , in one of 21 ( 5 % )
papillary thyroid cancer samples . -
pubmed
The structure of the active site features the facial
triad of protein ligands consisting of the side chains of two
histidine residues and one
aspartate residue that is common to many
non - heme Fe ( II ) oxygenases . -
pubmed
X - ray absorption spectroscopy ( XAS ) of metallated (
Fe and
Ni ) samples of
ABH2 was used to investigate the mechanism of
ABH2 and its inhibition by
Ni ( II ) ions . -
pubmed
This aspect is not altered in the
Ni ( II )-substituted
enzyme , and both metals are shown to bind the cofactor . -
pubmed
falciparum induces alterations to the infected RBC morphology and bio - mechanical properties to eventually rupture the host cells through parasitic and host derived
proteases of
cysteine and
serine families . -
pubmed
We used previously reported broad - spectrum inhibitors (
E64d ,
EGTA - AM and
chymostatin ) to inhibit these
proteases and impede
rupture to analyze mechanical signatures associated with parasite escape . -
pubmed
Treatment of late - stage iRBCs with
E64d and
EGTA -
AM prevented
rupture , resulted in no major RBC cytoskeletal reconfiguration but altered schizont morphology followed by dramatic re - distribution of three - dimensional refractive index ( 3D - RI ) within the iRBC . -
pubmed
We show that
E64d and
EGTA -
AM supported PV breakdown and the resulting elevated fluctuations followed non - Gaussian pattern that resulted from direct merozoite impingement against the iRBC membrane . -
pubmed
ER - directed treatments include lowering ligand concentration by using
aromatase inhibitors ( AI ) and blocking the receptor with agents like
tamoxifen (
TAM ) or
fulvestrant (
FUL ) . -
pubmed
RESULTS : As expected ,
tumor FES uptake declined more markedly on
ER blockers (
TAM and
FUL , average 54 % decline ) compared with a less than 15 % average decline on
estrogen - depleting AIs ( P & lt ; 0.001 ) . -
pubmed
INTERVENTION(S ): Daily
gonadotropin - releasing hormone antagonist (
GnRH , 0.25 mg ) used from day 6 of stimulation onward , final oocyte maturation triggered by administration of 10,000 IU of
human chorionic gonadotropin (
hCG ) as soon as & gt;= 3 follicles & gt;= 17 mm were present , and hormone assessment performed at initiation of stimulation , on the first day after menstruation had stopped , on the day of antagonist initiation , and on the day of
hCG administration . -
pubmed
CONCLUSION(S ): In recombinant
follicle - stimulating hormone /
gonadotropin - releasing hormone antagonist in vitro fertilization / intracytoplasmic sperm injection cycles , a lower probability of pregnancy is associated with a higher exposure to
progesterone during the menstruation - free interval . -
pubmed
However , membrane
cholesterol also plays a role in the formation and aggregation of
amyloid - beta . -
pubmed
During the fifth year of follow up , incident
cardiovascular diseases , ABI , and mortality were documented , and related to
ACE genotype adjusting for age , smoking ,
alcohol , dietary intake , physical activity ,
body mass index , and use of
ACE inhibitors . -
pubmed
A reporter gene construct employing a synthetic
oestrogen response element predicted to specifically interact with
oestrogen receptors (
ER ) was constructed . -
pubmed
Porous and trigonal
TiO ( 2 ) nanoflakes ( p -
TiO ( 2 ) ) have been synthesized via a simple hydrothermal calcination process , and characterized by X - ray diffraction ( XRD ) , transmission electron microscopy (
TEM ) , and UV - vis absorption / reflection spectroscopy ( UV - vis ) . -
pubmed
Inhibition of
mitochondrial complex I by various non - steroidal anti - inflammatory drugs and its protection by
quercetin via a coenzyme Q - like action . -
pubmed
A major objective of the present study was to investigate whether in vitro the NSAIDs ,
aspirin ,
indomethacin ,
diclofenac ,
piroxicam and
ibuprofen , which feature different chemical structures , are able to inhibit
mitochondrial complex I. -
pubmed
In addition , the present study shows that
quercetin ( 5 - 10 muM ) can behave as a " coenzyme Q - mimetic " molecule , allowing a normal electron flow along the whole electron transporting chain ( complexes I ,
II , III and
IV ) . -
pubmed
The organic
hydroperoxide resistance protein
Ohr has been identified in numerous bacteria where it functions in the detoxification of organic
hydroperoxides , and expression of
ohr is often regulated by a
MarR - type regulator called
OhrR. -
pubmed
The effect of oscillatory decrease in the heart rate and
hemoglobin oxygen saturation ( SaO2 ) during the submaximal workload ( bicycle ergometry test ) was demonstrated in high - performance athletes and volunteers practicing healthy life style . -
pubmed
Absence of both
MDR1 (
ABCB1 ) and
breast cancer resistance protein (
ABCG2 ) transporters significantly alters
rivaroxaban disposition and central nervous system entry . -
pubmed
Rivaroxaban is a novel
factor 10a inhibitor , where hepatic metabolism and renal clearance account for its overall disposition . -
pubmed
As renal
rivaroxaban clearance exceeds glomerular filtration rate , we suggested that active secretion by efflux transporters
P - glycoprotein (
MDR1 ) and
breast cancer resistance protein (
BCRP ) contributes to
rivaroxaban clearance . -
pubmed
The ability of
MDR1 and
BCRP efflux transporters to mediate
rivaroxaban transport in vitro was assessed in polarized cell monolayers . -
pubmed
Rivaroxaban is a shared substrate of
MDR1 and
BCRP . -
pubmed
In vivo ,
MDR and
BCRP function synergistically to modulate
rivaroxaban disposition and appear to be particularly relevant to limiting its central nervous system entry . -
pubmed
These data have important implications for safety and efficacy of anticoagulation therapy with
rivaroxaban as many drugs in clinical use are known
MDR1 inhibitors and loss - of - function polymorphisms in
BCRP are common . -
pubmed
We have here demonstrated for the first time that
nitrate not only accelerates testa
rupture of non-
AR seeds but also modifies expression pattern of the cell - wall remodeling proteins (
mannanases ;
SoMAN6 and
SoMAN7 ) and key genes belonging to metabolism and signaling of ABA (
SoNCED6 ,
SoNCED9 ,
SoCYP707A2 and
SoABI5 ) and
GAs (
SoGA3ox ,
SoGA20ox ,
SoGA2ox and
SoRGL2 ) . -
pubmed
The expression of
SoMAN6 and
SoMAN7 were positively altered by
nitrate . -
pubmed
Although
ABA synthesis seems apparent at the start of non -
AR seed imbibition , taken together the results of
SoNCED6 ,
SoNCED9 and
SoCYP707A2 expression seem to suggest that
nitrate leads to a strong net
ABA decrease . -
pubmed
Likewise ,
nitrate positively affected the
SoABI5 expression when the
SoNCED9 expression was also stimulated . -
pubmed
By contrast , at the early and final of imbibition ,
nitrate clearly inhibited the
SoABI5 expression . -
pubmed
The expression of
SoGA2ox6 and
SoGA3ox2 are strongly inhibited by
nitrate whereas of
SoGA20ox6 was stimulated . -
pubmed
On the other hand ,
SoRGL2 transcript level decreased in the presence of
nitrate . -
pubmed
The
nitrate , in cross - talk with the
AR network likely increases the favorable molecular conditions that trigger germination . -
pubmed
Using the nano - porous pseudo
carbon paste electrode ( Nano - PPCPE ) as the working electrode , mixing
L - glutamate oxidase (
L - GLOD ) ,
catalase (
Cat ) and
bovine serum albumin (
BSA ) with
phosphate buffer (
PB , pH = 7.4 ) , followed by cross - linking with
glutaraldehyde , a novel
L - glutamate electrochemical biosensor was successfully formed . -
pubmed
Cytotoxicity was evaluated using
MTT reduction ,
lactate dehydrogenase leakage and
neutral red uptake assays , for different concentrations and combinations of both agents , at 24 and 48 h. Apoptosis was also assessed using fluorescence microscopy and through the evaluation of
caspases 8 ,
9 , and 3 activities . -
pubmed
Administration of either
bovine thyrotropic hormone (
bTSH ) or
ovine growth hormone (
oGH ) had no effect on
thyroid hormone content of larvae challenged during the yolk absorption period , suggesting that the thyroid tissue was not responsive to exogenous
bTSH challenge at this time , and that
oGH - sensitive 5'-monodeiodination was either not present or at levels that were too low to cause an elevation in total T3 content , or that the substrate levels were insufficient to permit a measureable increase in whole body T3 content . -
pubmed
In this regard , the purpose of this Forum Article is to more visibly highlight the significant contributions of inorganic chemistry to nuclear imaging by detailing the development of five metal - based imaging agents :
( 64 ) Cu - ATSM ,
( 68 ) Ga - DOTATOC ,
( 89 ) Zr -
transferrin ,
( 99m ) Tc - sestamibi , and
( 99m ) Tc - colloids . -
pubmed
In general the substances which enhanced cell elongation also enhanced the development of
invertase , but generally they did not enhance the increase in
peroxidase activity or have any effect on the decrease in RNA content.
[( 14 ) C ] cinnamic acid was continuously incorporated into the cell walls of elongating segments from which it could only be partially removed with
NaOH , pronase , or
HClO4 . -
pubmed
Here , we present a new strategy applying MALDI -
TOF MS in positive ion mode for semi - qualitative and quantitative analysis of
CS /
DS derived
disaccharide units . -
pubmed
The levels of
alanine aminotransferase (
ALT ) ,
aspartate aminotransferase (
AST ) , total
cholesterol (
TC ) ,
triglyceride (
TG ) ,
high density lipoprotein cholesterol (
HDL - C ) ,
low density lipoprotein cholesterol (
LDL - C ) were detected in the serum and liver tissue ; Changes in fat deposition in liver tissue were determined by oil red O staining . -
pubmed
The molecular mechanisms underlying the targets of
curcumin are diverse and involve combinations of multiple signaling pathways , including
NF - kappaB and
STAT3 signaling . -
pubmed
It was established that in all experimental versions rapidly absorbed from the culture medium
amino acids :
cysteine and
cystine ,
serine and
aspartic acid ,
valine and
tryptophan ,
proline and
hydroxyproline , which are involved in the synthesis of proteins , in particular
collagen . -
pubmed
This mutation ( ' DeltaE ' ) removes a single
glutamic acid from the encoded protein ,
torsinA. -
pubmed
Based on the PQA adherence measure (
PDC & gt;=0.8 ) , a significantly higher proportion of
rivaroxaban users ( 72.7 % ) was found to be adherent compared to
dabigatran ( 67.2 % : p & lt ; 0.001 ) and
apixaban ( 69.5 % : p & lt ; 0.001 ) users . -
pubmed
Adipocyte - like differentiation was confirmed by a combination of positive
Oil Red O and
adipophilin immunohistochemical staining . -
pubmed
Substitution of
phenylalanine residues by
tryptophan introduced endotoxin neutralization capacity in inactive truncated
CATH-2 derived peptides . -
pubmed
In contrast ,
amino acid substitution of
phenylalanine by
tyrosine abrogated endotoxin neutralization activity of
CATH-2 analogs . -
pubmed
The
anthrax lethal factor (
LF ) is a
zinc dependent metalloproteinase that cleaves the majority of
mitogen - activated protein kinase kinases and a member of
NOD - like receptor proteins , inducing cell apoptosis . -
pubmed
Tannase - mediated biotransformation assisted separation and purification of
theaflavin and
epigallocatechin by high speed counter current chromatography and preparative high performance liquid chromatography : A comparative study . -
pubmed
A large scale isolation and purification of
theaflavin (
TF ) and
epigallocatechin (
EGC ) has been successfully developed by
tannase - mediated biotransformation combining high - speed countercurrent chromatography . -
pubmed
After
tannase hydrolysis of a commercially available
theaflavins extract ( TE ) , the content of
TF and
EGC in
tannase - mediated biotransformation product (
TBP ) achieved approximately 3 times enrichment . -
pubmed
OBJECTIVE : The objective of this study was to investigate the relationship between level of
18F - fluorodeoxyglucose (
18F - FDG ) accumulation in primary
breast tumour assessed by positron emission tomography / computed tomography ( PET /
CT ) and histopathological and clinical prognostic factors . -
pubmed
Transient receptor potential cation channel , subfamily C , member 1 (
TRPC1 ) activates
Ca2 + entry upon store depletion in a variety of cell types . -
pubmed
Moreover , knockdown of
TRPC1 decreased
Ca2 + entry to the cytoplasm of hDPCs . -
pubmed
In wild - type reaction centers , the triplet energy transfer efficiency from P to the carotenoid was essentially unity at room temperature and at 77 K. However , in the
M182HL mutant reaction centers , both the rate and efficiency of triplet energy transfer were decreased at room temperature , and at 77 K , no triplet energy transfer was observed , attributable to a higher triplet state energy of the
bacteriopheophytin that replaces
bacteriochlorophyll in this mutant . -
pubmed
In this work , a fully
tin - based , mixed - organic - cation
perovskite absorber (
FA ) x ( MA)1 - x
SnI3 ( FA =
NH2CH =
NH2 + , MA =
CH3NH3 + ) for lead - free
perovskite solar cells ( PSCs ) with inverted structure is presented . -
pubmed
By optimizing the ratio of
FA and MA cations , a maximum power conversion efficiency of 8.12 % is achieved for the ( FA)0.75(MA)0.25SnI3 - based device along with a high open - circuit voltage of 0.61 V , which originates from improved
perovskite film morphology and inhibits recombination process in the device . -
pubmed
An
insulin tolerance test was used unless contraindicated , then the
GHRH -
arginine test and Synachten test were used . -
pubmed
GABA and
malate appear to interact with
ALMTs in a complex manner to regulate each other 's transport , suggestive of a role for
ALMTs in communicating metabolic status . -
pubmed
Finally , the
alkaline phosphatase activity of the cells was found to increase with increasing content of TCP , which provides an encouraging proof of concept for the use of
curcumin - loaded synthetic biomaterials in bone remodeling . -
pubmed
Over - expression of
AtPAP2 resulted in faster growth and increased biomass in several plant species , indicating its great potential for crop improvement of
phosphate use and yield . -
pubmed
Glutathione superoxide dismutase , antioxidant capacity , and total
superoxide dismutase were higher , whereas
malondialdehyde was lower in F group than in C and U groups from 1 to 21 days and from 22 to 42 days ( P & lt ; 0.05 ) . -
pubmed
Nitrogenase - like enzymes play a vital role in the reduction of the conjugated ring systems of diverse
tetrapyrrole molecules . -
pubmed
The biosynthesis of all bacteriochlorophylls involves the two - electron reduction of the
C7 - C8 double bond of the green pigment
chlorophyllide , which is catalyzed by the
nitrogenase - like two - component
metalloenzyme chlorophyllide oxidoreductase (
COR ) ; whereas in all methanogenic microbes , another
nitrogenase - like system ,
CfbC / D , is responsible for the sophisticated six - electron reduction of
Ni2 + - sirohydrochlorin a ,
c - diamide in the course of
coenzyme F430 biosynthesis . -
pubmed
The first part of this chapter describes the production and purification of the
COR components (
BchY / BchZ ) 2 and
BchX2 , the measurement of
COR activity , and the trapping of the ternary COR complex ; and the second part describes the strategy for obtaining homogenous and catalytically active preparations of
CfbC2 and
CfbD2 and a suitable method for extracting the reaction product
Ni2 + - hexahydrosirohydrochlorin a ,
c - diamide . -
pubmed
The development of
somatostatin analogs for the treatment of pituitary
Cushing ' s disease has been based on
somatostatin receptor expression analyses of small cohorts of
pituitary adenomas . -
pubmed
SSTR2A ,
SSTR3 , and
SSTR5 seem to be most suitable biomarkers for a targeted therapy with
somatostatin analogs . -
pubmed
Compound
alpha - keto acid tablet supplementation alleviates
chronic kidney disease progression via inhibition of the
NF - kB and
MAPK pathways . -
pubmed
7 - Ethyl-10 - hydroxy - camptothecin (
SN-38 ) , an efficient
topoisomerase inhibitor , is the biological metabolite of
irinotecan used as the first - line chemotherapy drug for
colon cancer . -
pubmed
SN-38 - BOC micelles could accumulate in
tumors due to the
EPR effect and exhibit a sustained release behavior , which was rapidly transformed to active
SN-38 by the acidic environment of
tumor tissues ( pH 5.5 - 6.8 ) , thus achieving efficient anti -
tumor activity . -
pubmed
Up - regulation of
HDACs , a harbinger of
uraemic endothelial dysfunction , is prevented by
defibrotide . -
pubmed
Both the
HDACs inhibitor
trichostatin A and DF prevented the up - regulation of the endothelial dysfunction markers induced by the uraemic milieu :
intercellular adhesion molecule-1 , surface
Toll - like receptor-4 ,
von Willebrand Factor and reactive
oxygen species . -
pubmed
HDACs appear as key modulators of the
CKD - induced
endothelial dysfunction as specific blockade by
trichostatin A or by DF prevents endothelial dysfunction responses to the
CKD insult . -
pubmed
Previous studies have shown that the
carbonic anhydrase inhibitor ( CAI )
dorzolamide can induce immediate dilatation of retinal arterioles and a sustained increase in retinal blood flow in
primary open - angle glaucoma . -
pubmed
Li81 has the unusual feature that it contains two
LINGO-1 binding sites : a classical site utilizing its complementarity - determining regions and a cryptic secondary site involving Li81 light chain framework residues that recruits a second
LINGO-1 molecule only after engagement of the primary binding site . -
pubmed
tuberculosis and/or
HIV - infection and investigated
TB antibody reactivities , function , and other host protein biomarkers in simultaneously obtained serum and plasma from serum separation ,
EDTA ,
heparin ,
acid citrate dextrose (
ACD ) , or mononuclear cell preparation (
CPT ) tubes which contain heparin and Ficoll . -
pubmed
However , the presence of
ACD resulted in slightly lower values than those obtained with serum in both indirect ( antibody reactivities to mycobacterial antigens ) and Sandwich ELISAs ( soluble
CD14 measurements ) . -
pubmed
The results revealed the adsorption equilibrium of
rhodamine B (
RhB ) and
Congo red (
CR ) onto RFB550 with initial concentrations of 30 mg L-1 at 25 C and 210 min , and the best adsorption was observed when the pH of the
RhB and
CR solutions was 3 and 5 , respectively . -
pubmed
Pseudo - second - order kinetics was the most suitable model for describing the adsorption of
RhB and
CR onto
RFB550 , indicating that the rate - limiting step was mainly chemical adsorption . -
pubmed
Thermodynamic parameters revealed the spontaneous adsorption of
RhB and
CR onto
RFB550 . -
pubmed
According to the results of the FTIR analysis , the
oxygen - containing functional groups and aromatic structures on the surface of RFB550 provided abundant adsorption sites for
RhB and
CR , and the adsorption mechanism was potentially related to the
hydrogen bonds and pi - pi bonds . -
pubmed
We show here that during the early stage of
viral infection , the acetylation of
TBK1 was increased , and the acetylation of
TBK1 at
Lys241 enhanced the recruitment of
IRF3 to
TBK1 . -
pubmed
HDAC3 directly deacetylated
TBK1 at
Lys241 and
Lys692 , which resulted in the activation of
TBK1 . -
pubmed
Deacetylation at
Lys241 and
Lys692 was critical for the kinase activity and dimerization of
TBK1 respectively . -
pubmed
Here , we developed a two - part repair strategy comprising a dual - modified ( oxidized and methacrylated ) glycosaminoglycan that can chemically adsorb an injectable interpenetrating network hydrogel composed of
fibronectin - conjugated
fibrin and
poly ( ethylene glycol ) diacrylate (
PEGDA ) to covalently bond the hydrogel to
AF tissue . -
pubmed
The concentrations of exposed PM2.5 , PM10 ,
NO2 and
O3 in the
PE group were significantly higher than those in the non - PE group , and they were risk factors of the
PE group in the first and second trimester of pregnancy respectively . -
pubmed
As a result , the adsorption capacity of
Cd2 + on MFB ( 17.4 mg g-1 ) was more than two times higher than that on
FB ( 7.2 mg g-1 ) , and the adsorption of
Cd2 + on MFB was controlled by physisorption and chemisorption . -
pubmed
The effect of MFB on the bioavailability of
Cd was investigated using a leaching test ( the European standard EN 12457 - 2 ) combined with a typical sequential extraction procedure ( the community bureau of reference ,
BCR ) . -
pubmed
,
thiazole ) should be introduced to
P1 ' and P4 sites in order to enhance H - bonds and hydrophobic interactions , respectively . -
pubmed
By contrast , individuals with
MetS had lower
high - density - lipoprotein cholesterol (
HDL - C ) counts and FPIS . -
pubmed
It appears that immunoreactivity of
beta - endorphin resides in the
COOH - terminal segment of residues ( 22 - -31 ) . -
pubmed
The data reported here indicate the presence of
B - cell epitopes within the
carboxy - terminal third of the
gp340 polypeptide chain . -
pubmed
Occurrence of an allosteric transition in the modification of
papain with
L-1 - acetyl-2,3 - dihydropyrrolo [ 2,3 - b ]- indole-2 - carboxamide . -
pubmed
When
papain was reacted with
L-1 - acetyl-2,3 - dihydropyrrolo [ 2,3 - b ] indole - 2 - carboxamide at pH 8.0 , inactivation occurred accompanied by modification of
Cys-25 in the active site . -
pubmed
This is supported by the fact that the presence of a moderate concentration ( a twenty - fold molar excess ) of
N alpha - acetyl - L - tryptophanamide over
papain accelerated the inactivation . -
pubmed
The extracytoplasmic region of the 270 - kDa
mannose 6 - phosphate /
IGF - II receptor is composed of 15 repeating domains and is capable of binding 2 mol of
mannose 6 - phosphate (
Man-6 - P ) . -
pubmed
These adrenal responses were blocked by
dexamethasone (
DEX ) suggesting that the effect of 4 -
APP occurred indirectly via stimulation of the adrenal gland by endogenous adrenocorticotrophic ( ACTH ) . -
pubmed
In all the above experiments , adrenal gland
cholesterol content and
apoE mRNA concentration were positively correlated ( r = 0.78 , P = 0.0001 ) . -
pubmed
In contrast ,
aminoglutethimide treatment , which blocks adrenal gland steroidogenesis and greatly increases adrenal gland
cholesterol content , was without effect on
apoE mRNA concentration . -
pubmed
This uncoupling of adrenal gland
cholesterol content and
apoE mRNA concentration suggests that
apoE mRNA expression and cellular
cholesterol are regulated independently by
ACTH . -
pubmed
Phosphoaminoacid analysis indicated that
pp19 is dephosphorylated on
serine residues . -
pubmed
DDC forms chelates with
copper and the microbicidal effectiveness of this drug is enhanced greatly by small amounts of
copper . -
pubmed
E loss , and
LDH leakage induced by
Ca2 + omission from the incubation medium . -
pubmed
Anaemia of
rheumatoid arthritis :
serum erythropoietin concentrations and red cell distribution width in relation to
iron status . -
pubmed
The mean
serum erythropoietin level ( 57.6 ( SD ) 27.3 ) U / l ) was sufficiently raised for the degree of
anaemia irrespective of the size of the marrow
iron stores . -
pubmed
The plasma
lactoferrin concentration ( mean ( SD ) 137.6 ( 109.9 ) micrograms / l ) was normal and did not differ significantly between the
iron deficient patients and those with adequate
iron . -
pubmed
The positive inotropic effects of the
bipyridine derivatives
amrinone and
milrinone and of the
benzimidazole compounds
sulmazole ,
pimobendane , and
UD - CG 212 Cl are due at least in part to inhibition of cardiac
phosphodiesterase activity and hence to an increase in myocardial
cyclic AMP (
cAMP ) content . -
pubmed
Influence of sympathetic discharge pattern on
norepinephrine and
neuropeptide Y release . -
pubmed
After blockade of
alpha - and beta - adrenoceptors by
phenoxybenzamine and
propranolol , respectively , which enhanced nerve stimulation - evoked overflow of both NE and
NPY - LI , the NE overflow from the muscle evoked by the irregular activity was slightly larger at 0.59 Hz but smaller at higher frequencies compared with that evoked by regular activity , whereas the detectable overflow of
NPY - LI was not largely influenced by the stimulation pattern . -
pubmed
The
hydroxyl radical has been spin trapped in microsomal and purified
NADPH - cytochrome P-450 reductase systems in the presence of
adriamycin ,
daunomycin and
mitomycin C. -
pubmed
The
hydroxy radical generation has been stimulated by the
Fe - EDTA complex and completely inhibited by
catalase . -
pubmed
Strain differences in
pituitary prolactin content : relationship to number of hypothalamic
dopamine neurons . -
pubmed
Strain differences in the synthesis and release of
prolactin would also be predicted since
dopamine released from the tuberoinfundibular neurons is the prolactin inhibitory factor which plays a role in the regulation of both
prolactin synthesis and release . -
pubmed
We conclude that strain differences in the number of tuberoinfundibular
dopamine neurons are inversely related to the number of immunocytochemically demonstrable
prolactin - containing cells in the anterior pituitary . -
pubmed
[ Effect of the humic and
fulvic acids of sapropel on the
NAD.H - oxidase activity of the liver mitochondria ] . -
pubmed
Nuclease P1 - mediated enhancement of sensitivity of
32P - postlabeling test for structurally diverse DNA adducts . -
pubmed
In the standard procedure , DNA is enzymatically digested to 3'-phosphorylated normal and adducted
mononucleotides , which are quantitatively
32P - labeled at their
5 '- hydroxyl groups by
T4 polynucleotide kinase - catalyzed [
32P]
phosphate transfer from
[ gamma-32P ] ATP . -
pubmed
Nuclease P1 cleaves
deoxyribonucleoside 3 '- monophosphates of normal
nucleotides to
deoxyribonucleosides which do not serve as substrates for
polynucleotide kinase , while most adducted
nucleotides were found to be totally or partially resistant to the 3'-dephosphorylating action of
nuclease P1 . -
pubmed
Amino acid sequencing of the N termini of the pre -
hGH and
pho -
hGH gene products revealed that both were processed correctly . -
pubmed
Electrophoretic analysis of these polypeptides on reducing and nonreducing
sodium dodecyl sulfate (
SDS)-
polyacrylamide (
PA ) gels indicates that periplasmic
hGH is monomeric and contains the same two
disulfide bonds as authentic
hGH . -
pubmed
Assignments of the six sets of aromatic ring protons and four high - field - shifted
methyl group protons of the C - terminal fragment of
calmodulin , residues 78 - 148 , was achieved by a combination of one and two - dimensional NMR spectroscopic methods . -
pubmed
In the glomerular layer ,
methionine - enkephalin immunoreactivity is observed in the
somata and dendrites of periglomerular cells whereas
substance P immunoreactivity is present in the
somata and dendrites of external tufted cells . -
pubmed
In the deeper layers ,
methionine - enkephalin immunoreactivity is observed in a mixed population of neurons which appear to be primarily granule cells , whereas
somatostatin immunoreactivity is present only in deep short axon cells . -
pubmed
Methionine - enkephalin- ,
substance P- , and
somatostatin - positive fibers of central origin are also observed in the main olfactory bulb . -
pubmed
The cells and culture medium were collected , and radioactive
proline incorporated into
collagen and
noncollagen protein was determined . -
pubmed
Prolyl hydroxylase activity was also increased significantly in the
bleomycin - treated cells . -
pubmed
Free and peptide - bound radioactive
hydroxyproline in the cells and medium was greatly increased in
bleomycin - treated cells , which indicates increased
collagen degradation . -
pubmed
The results demonstrate that , although
collagen synthesis in lung fibroblasts is increased by
bleomycin , the newly synthesized
collagen is rapidly degraded in both the cell layer and the medium . -
pubmed
All hand
wounds should be cultured aerobically and anaerobically and empiric antibiotic therapy should include a
penicillinase - resistant
penicillin or
cephalosporin in combination with
penicillin G. -
pubmed
Prolonged combined in vivo pre - treatment with
luteinizing hormone - releasing hormone (
LRH ) and
oestradiol benzoate causes long - lasting suppression of the autonomous and the
LRH - stimulated secretion of
luteinizing hormone and
follicle stimulating hormone . -
pubmed
The in vivo distribution of
iron between the binding sites of
transferrin was examined . -
pubmed
Independent methods , including measurement of the
transferrin profile after isoelectric focusing and cross immunoelectrophoresis , and determination of the
iron content in the separated fractions were in agreement that there was a random distribution of
iron on binding sites . -
pubmed
The data indicate that the distribution of
apo - , monoferric , and diferric transferrins is predictable on the basis of the plasma
transferrin saturation and negate the concept that
iron loading of
transferrin in vitro is a selective process with possible functional consequences in tissue
iron delivery . -
pubmed
The
proteinase activity was completely inhibited by
di - isopropyl fluorophosphate ,
leupeptin and antipain , indicating that the
proteinase bound to
alpha 1 - macroglobulin is a
serine proteinase . -
pubmed
Two protein bands ( 62 and 59 kDa ) of the complex were labelled with
[ 3H ] diisopropyl fluorophosphate and both bands cross - reacted with
anti -( mast - cell tryptase ) antibody . -
pubmed
In the lean subjects ,
tyrosine phosphorylation of the
insulin receptor and
insulin receptor substrate-1 (
IRS-1 ) , measured by immunoblotting with anti - phosphotyrosine antibodies , was significantly increased by
insulin at all time points . -
pubmed
RESULTS : All compounds examined in this study showed high and similar affinity for
alpha 1 adrenoceptor subtypes , with the exception of
5 - Methyl - urapidil , which was selective for
alpha 1C ( pKi = 9.3 ) over
alpha 1B ( pKi = 7.2 ) and
alpha 1A ( pKi = 8.1 ) . -
pubmed
Doxazosin ,
terazosin ,
alfuzosin , and
tamsulosin were potent antagonists of
phenylephrine responses and in vivo derived " pseudo pA2 " determinations showed that the drugs did not discriminate between
prostatic and vascular receptors . -
pubmed
Facilitation of ICa was not altered ( when present ) or favoured ( when absent ) by the
cAMP - dependent phosphorylation of
Ca2 + channels promoted by
isoprenaline or by intracellular application of
cAMP or of the catalytic subunit of
protein kinase A ( C - sub ) . -
pubmed
Following intracellular application of inhibitors of
protein kinase A in combination with a non - hydrolysable
ATP analogue , ICa consisted predominantly of ICa , sc and no facilitation was observed . -
pubmed
The
calmodulin antagonist
naphthalenesulphonamide had no effect on facilitation . -
pubmed
Inositol trisphosphate /
Ca2 + as a major signal transduction pathway from
bradykinin receptors . -
pubmed
The initial signal transduction from
bradykinin receptors to
acetylcholine secretion is composed of the
inositol-1,4,5 - trisphosphate - dependent
Ca2 + pathway despite multiple pathways in the late phase . -
pubmed
The interaction of the B1 domain with
IgGFc was investigated by
1H-15N correlation spectroscopy . -
pubmed
diabetes ( RR = 1.67 and 1.53 in men and women , respectively ) , serum
cholesterol ,
low density lipoprotein cholesterol and serum
apo B in men ( RR = 1.24 ) , serum
triglycerides in women ( RR = 1.23 ) ,
high density lipoprotein cholesterol in men ( RR = 0.82 ) ,
lipoprotein ( a ) in women ( RR = 1.99 ) , and poorer self - rating of health ( RR = 1.48 and 1.93 in men and women , respectively ) . -
pubmed
Approach to genotyping errors caused by nontemplated
nucleotide addition by
Taq DNA polymerase . -
pubmed
Thermostable
DNA polymerases can catalyze nontemplated addition of a
nucleotide to the 3 ' end of amplification products . -
pubmed
These findings indicate that the mutation is specific for
cytochrome c oxidase and does not affect the biosynthesis of
heme A. -
pubmed
We found that the
BrdU in vivo method , coupled with bivariate
FCM for measurements , is a valuable approach for a ' dynamic ' assessment of
tumour cell proliferation in the clinical setting . -
pubmed
Galactose exposed lenses both in vitro and in vivo showed a 5 - 9 - fold increase in
crystallin bound
Schiff base - linked radioactivity when incubated with
1 - 14C - labeled ASA or
DHA . -
pubmed
The mutagenic effect was found only in
acetone extracts of urinary filtrate in the presence of promutagenic activating fraction
S9 . -
pubmed
Based on these findings ,
rhizoxin , a microbial metabolite that contains two
epoxide groups and exhibits anti -
tubulin activity , was tested for anti - angiogenic activity in a CAM assay system . -
pubmed
The two
migration inhibitory factor - ( MIF )- related protein-8 (
MRP8 ;
S100A8 ) and
MRP14 (
S100A9 ) are two
calcium - binding proteins of the
S100 family . -
pubmed
This review addresses recent developments regarding the
MRP14 - mediated promotion of leukocyte - endothelial cell - interactions and the characterization of
MRP8 / 14 heterodimers as a
fatty acid binding protein complex . -
pubmed
Sample lipooligosaccharides ( LOS ) , was prepared by a modification of the hot
phenol - water method from which
de - O - acetylated LOS and
oligosaccharide ( OS ) components were analyzed by ES -
MS - CID -
MS and ES - MSnin a triple quadrupole and an ion trap mass spectrometer , respectively . -
pubmed
Mild
acid hydrolysis to release OS also hydrolyzed the beta(1 - ->6 ) glycosidic linkage of lipid A. OS structures were determined by collisional and resonance excitation combined with
MS and multistep MSn which provided sequence information from both neutral loss , and nonreducing terminal fragments . -
pubmed
With intrapipette ( -)Bay
K 8644 ( 1 microM ) , mean open time was best fit by two exponentials , tau(of ) = 0.9+/-0.2 msec ( n = 10 ) and
tau(os ) = 13.4+/-2.6 msec ( n = 10 ) ; mean close time values were
tau(cf ) = 0.6+/-0.1 msec ( n = 10 ) and
tau(cs ) = 9.8+/-1.9 msec ( n = 10 ) , respectively . -
pubmed
However ,
CGP 2881 consistently inhibited
CuSO4 (
Cu2 +)- and macrophage ( MO)-induced oxidation of
human low density lipoproteins (
LDL ) more potently than equimolar concentrations of
probucol . -
pubmed
CGP 2881 ( 1 mumol / l ) prolonged the lag phase of
diene formation during
Cu ( 2 +)-induced
LDL oxidation by 3.4 versus 1.5 - fold prolongation with 1 mumol / l
probucol ( P & lt ; 0.05 vs
CGP 2881 ) . -
pubmed
The IC50 for MO - induced oxidation of
LDL ( TBARS ) was 0.64 mumol / l. In contrast , 1 mumol / l
probucol failed to inhibit MO - induced oxidation of LDL . -
pubmed
Five days of orally administered
CGP 2881 reduced plasma total
cholesterol and
LDL cholesterol levels to 55 and 54 % of vehicle - treated controls , respectively ( P & lt ; 0.05 ) . -
pubmed
In contrast ,
probucol had no appreciable effect on plasma total
cholesterol or
LDL cholesterol levels , unless administered for longer than 5 days . -
pubmed
CGP 2881 may be useful as a new antioxidant tool in the effort to better understand the atherogenicity of oxidized LDL (
oxLDL ) . -
pubmed
Photoregulation of the
DNA Polymerase Reaction by
Oligonucleotides Bearing an
Azobenzene We should like to thank Professor Kazunari Taira for valuable comments . -
pubmed
Computer simulations are presented that resolve the paradoxical finding that while
ACE inhibitors are effective , genetic variations in the expression of the
ACE gene do not affect blood pressure . -
pubmed
The
DES - induced change in
AR immunoexpression was confirmed by Western analysis for the testis . -
pubmed
Treatment - induced changes in
AR expression were paralleled by changes in Leydig cell volume per testis ( 91 % reduction in the 10 - microg
DES group ; no change in the 1 - microg
DES group ) . -
pubmed
Coadministration of TE with
DES prevented
DES - induced loss of
AR immunoexpression ( confirmed for testis by Western blot analysis ) . -
pubmed
The
peroxiredoxins (
Prx ) are a family of 25 kDa peroxidases that can reduce
H2O2 using an electron from
thioredoxin (
Trx ) or other substances . -
pubmed
Expression of the
insulin gene is highly specific to pancreatic beta cells and is upregulated mainly by
PDX-1 and
BETA2 /
NeuroD depending on the extracellular
glucose concentration . -
pubmed
Reporter gene analyses using pancreatic HIT - T15 cells revealed that the
glucose - dependent
insulin promoter activity was blocked by glucocorticoids ,
dexamethasone (
DEX ) and
hydrocortisone , in a dose - dependent manner . -
pubmed
Moreover , because
CGP 52608 is a ligand of
RZR / ROR receptors and the latter are considered by some investigators as nuclear binding sites for
melatonin , our data suggest the involvement of these receptors in the pro - apoptotic effect of
melatonin . -
pubmed
Structural basis for the recognition of
isoleucyl - adenylate and an antibiotic ,
mupirocin , by
isoleucyl - tRNA synthetase . -
pubmed
A structural comparison of the IleRS.Ile - AMS complex with the
adenylate complexes of other
aminoacyl - tRNA synthetases revealed the common recognition mode of
aminoacyl - adenylate by the
class I aminoacyl - tRNA synthetases . -
pubmed
The
Ile - AMS and
mupirocin , which have significantly different chemical structures , are recognized by many of the same
amino acid residues of the
IleRS , suggesting that the antibiotic inhibits the enzymatic activity by blocking the binding site of the high energy intermediate ,
Ile - AMP . -
pubmed
In contrast , the two
amino acid residues that concomitantly recognize
Ile - AMS and
mupirocin are different between the eubacterial / archaeal
IleRSs and the eukaryotic
IleRSs . -
pubmed
AChE staining was located in the endoplasmic reticulum of geniculate
ganglion neuronal
somata , and extracellularly , surrounding labeled chorda tympani terminal fibers and boutons in the NST . -
pubmed
AChE staining was reduced in the
dextran - labeled chorda tympani fibers and terminals as well as adjacent non - labeled profiles 2 weeks following nerve transection and
dextran application . -
pubmed
Within the composition of the ration , the levels of rumen degradable protein (
RDP ) and non - structural
carbohydrates ( NSC ) were changed ( diet H : 74 % RDP and 38 % NSC ; diet M : 57 % RDP and 32 % NSC ; diet L : 48 % RDP and 23 % NSC ) . -
pubmed
Different test agents : muscle stimulation (
MS ) , arterial injection of
KCl or
lactic acid and tendon vibrations , were used to activate muscle afferents . -
pubmed
Both acute and chronic
hypoxaemia suppressed the
post - MS activation of groups III and
IV muscle afferents and significantly depressed the response of these afferents to
KCl and
lactic acid . -
pubmed
Penzo ( a ) pyrene ,
7,12 - dimethyl benz ( a ) anthracene and
pentacene effectively inhibit the specific
3H - estradial binding to
rat uterine estrogen receptor(P & lt ; 0.01 ) . -
pubmed
Whereas
benz ( a ) anthracene and
chrysene can not inhibit the specific
3H - estradial binding to
rat uterine estrogen receptor(P & gt ; 0.05 ) . -
pubmed
Insulin affects not only
glucose metabolism , but also protein synthesis and cell growth . -
pubmed
Insulin - induced
tyrosine phosphorylation of
insulin receptor beta subunit (
IRbeta ) and
insulin receptor substrate-1 (
IRS-1 ) and
tyrosine /
threonine phosphorylation of
p44 / 42 MAPK (
ERK-1 / 2 ) were evaluated . -
pubmed
However ,
tyrosine phosphorylation of
IRS-1 in the soleus muscle of
FFR was significantly reduced to 80 % ( p<0.001 ) of that in controls . -
pubmed
Gelatin - containing native - PAGE gels indicated the presence of several
aspartyl ,
cysteine , and
serine protease forms and confirmed the spatial organization of the proteolytic digestive process . -
pubmed
Serum
parathyroid hormone was suppressed ,
25 - OH vitamin D was within the normal range , and
1,25 -( OH )( 2 ) vitamin D was elevated . -
pubmed
This study evaluated the effect of
fenoldopam , a selective
dopamine (
DA1 ) agonist , on renal blood flow and renal tubular function following
renal ischemia induced by suprarenal aortic cross - clamping . -
pubmed
The mean
creatinine increase from baseline at 6 hr and 18 hr after cross -
clamp removal for the
fenoldopam - treated group was significantly less than that in the control group ( p & lt ; 0.001 ) . -
pubmed
METHODS : Claims data from a large US managed care organization from July 1 , 1998 , through June 30 , 2000 , were analyzed for adult members with continuous enrollment , & gt;or=1 prescription drug claim , & gt;or=2 sets of fasting
low - density lipoprotein cholesterol (
LDL - C ) laboratory results , and no lipid - lowering prescription claims at any time & lt;or=12 months before the date of the first set of
LDL - C laboratory results . -
pubmed
Similar results were observed for subanalyses of subjects with
diabetes mellitus or
coronary heart disease (
CHD ) ; individuals who received more efficacious statin regimens were more likely to reach the National
Cholesterol Education Program Adult Treatment Panel
II LDL - C goal of & lt;or=100 mg / dL ( P & lt ; 0.05 for moderate- or low - efficacy regimens vs high - efficacy statins in each model ) . -
pubmed
This phenotype , and the fact that this mutation confers resistance to
concanamycin , suggests that
V - ATPase is the main ( or only ) target for the antibiotics . -
pubmed
The role of recombinant
lysine -
plasminogen and recombinant
urokinase and
sulfur hexafluoride combination in inducing
posterior vitreous detachment . -
pubmed
PURPOSE : To determine the optimal method of generating
plasmin in vitreous using recombinant
lysine -
plasminogen and recombinant
urokinase and to determine its efficacy in inducing
posterior vitreous detachment when combined with
sulfur hexafluoride . -
pubmed
Intravitreal administration of recombinant
lysine -
plasminogen and recombinant
urokinase did not cause any
toxicity findings up to concentrations of 100 microg and 200 IU , respectively , on funduscopy , electroretinography , and histopathologic studies . -
pubmed
When combined with
sulfur hexafluoride injection , separate intravitreal administrations of 75 microg/0.1 mL of recombinant
lysine -
plasminogen and 15 IU/0.1 mL of recombinant
urokinase induced complete
posterior vitreous detachment in 75 % of the eyes compared with 13 % in eyes that received
sulfur hexafluoride injection combined with
balanced salt solution , recombinant
lysine -
plasminogen , or recombinant
urokinase . -
pubmed
CONCLUSIONS :
Plasmin was effectively generated in the vitreous after separate intravitreal administrations of recombinant
lysine -
plasminogen and recombinant
urokinase . -
pubmed
Intrastriatal administration of the
succinate dehydrogenase (
SDH ) inhibitor
malonate produces
neuronal injury by a " secondary excitotoxic " mechanism involving the generation of reactive
oxygen species ( ROS ) . -
pubmed
Inhibition of the catabolic enzyme
monoamine oxidase (
MAO ) also attenuates
striatal lesions induced by
malonate . -
pubmed
In addition to forming
3,4 - dihydroxyphenylacetic acid , metabolism of
dopamine by
MAO generates
H2O2 , suggesting that
dopamine metabolism may be a source of ROS in
malonate toxicity . -
pubmed
These findings indicate that normal levels of
MAO - A participate in expression of
malonate toxicity by a mechanism involving oxidative stress . -
pubmed
AIM : To investigate the importance of direct contact between Kupffer cells ( KCs ) and hepatocytes ( HCs ) during hepatic inflammatory responses , and the effect of
leflunomide 's active metabolite , A(771726 ) , on
cytokines in KCs , HCs and KC cocultures ( DC cocultures ) . -
pubmed
Leflunomide 's active metabolite , A(771726 ) , had significantly inhibitory effect on
TNF - alpha and
IL-1 at protein and transcription levels , and the reduced production of
IL-1 by A(771726 ) was associated with the inhibitory action of A(771726 ) on
TNF - alpha . -
pubmed
CONCLUSION :
Leflunomide can inhibit hepatocyte damage by inhibiting proinflammatory
cytokine release from KCs . -
pubmed
Reactive
oxygen species - dependent
TNF - alpha converting enzyme activation through stimulation of
5 - HT2B and
alpha1D autoreceptors in neuronal cells . -
pubmed
This was associated with a dose - dependent ( 10 - 40 micromol / L ) upregulation of cytosolic
phospholipase A ( 2 ) (
cPLA ( 2 ) ) expression and
arachidonic acid release . -
pubmed
In contrast ,
vitamin E dose dependently ( 10 - 60 micromol / L ) inhibited
cyclooxygenase (
COX ) activity but did not affect the expression of either
COX-1 or
COX-2 , indicating that the effect of
vitamin E on
COX activity was
post - translational . -
pubmed
BACKGROUND :
Sildenafil inhibits
phosphodiesterase 5 (
PDE5A ) to elevate intracellular
cGMP and to induce vasodilation . -
pubmed
In the initial
dobutamine test , systolic and diastolic function improved similarly in both treatment groups ( eg , peak power index rose 80+/-28 % in the placebo group and 82+/-31 % in the
sildenafil group ; P =
NS ) . -
pubmed
CONCLUSIONS :
PDE5A inhibition by
sildenafil blunts systolic responses to beta - adrenergic stimulation . -
pubmed
The expression of
CA IV ,
CA VII and
CA - RP VIII was somewhat similar : they were expressed in the cortex , hippocampus and midbrain structures and their expression did not change after the
kainic acid treatment . -
pubmed
Kainic acid stimulated
CA XII expression throughout the cortical layer I. -
pubmed
Low - temperature Fourier transform infrared ( FTIR ) spectroscopy is used to study squid
rhodopsin at 77 K in investigating structural changes in the
Schiff base region upon photoisomerization . -
pubmed
We report here a novel mechanism for
glucose - mediated activation of
carbohydrate response element binding protein (
ChREBP ) , a basic helix - loop - helix /
leucine zipper ( bHLH /
ZIP ) transcription factor of
Mondo family that binds to
carbohydrate response element in the promoter of some
glucose - regulated genes and activates their expression upon
glucose stimulation . -
pubmed
Structure - function analysis of
ChREBP in a highly
glucose - sensitive system using
GAL4 -
ChREBP fusion constructs revealed a
glucose - sensing module ( GSM ) that mediates
glucose responsiveness of
ChREBP . -
pubmed
We have identified a new mechanism accounting for
glucose responsiveness of
ChREBP that involves specific inhibition of the transactivation activity of
GRACE by LID under low
glucose concentration and reversal of this inhibition by
glucose in an orientation - sensitive manner . -
pubmed
Chronic
fluoxetine treatment increases the expression of
PSA -
NCAM in the medial prefrontal cortex . -
pubmed
As the expression of
PSA -
NCAM is increased by
serotonin in other cerebral regions , antidepressants acting on
serotonin reuptake may influence
PSA -
NCAM expression and thus counteract the effects of
depression by modulating neuronal structural plasticity . -
pubmed
The effects of
fluoxetine treatment for 14 days on mPFC
PSA -
NCAM expression have also been analyzed . -
pubmed
In general , chronic
fluoxetine treatment induces significant increases in the number of
PSA -
NCAM immunoreactive neurons and in neuropil immunostaining and coadministration of the
5 - HT3 antagonist
ondansetron blocks the effects of
fluoxetine on
PSA -
NCAM expression . -
pubmed
These results indicate that
fluoxetine , acting through
5 - HT3 receptors , can modulate
PSA -
NCAM expression in the mPFC . -
pubmed
The peptides were designed to interact electrostatically with siRNA , selecting the
TAT peptide domain of HIV ( RKKRRQRRR ) and an
oligolysine (
Lys(9 ) ) . -
pubmed
In another series of experiments , the geometric mean IC(50)s ( n = 33 ) for
fosmidomycin and
TH II46 were 413 nM and 249 nM , respectively . -
pubmed
FR900098 and
TH II46 were 2.6 and 1.7 times more potent , respectively , than
fosmidomycin . -
pubmed
RESULTS : The women classified as having IR had a significantly higher
body mass index ( BMI ) and free
androgen index ( FAI ) and showed significantly lower
SHBG and
high - density lipoprotein (
HDL ) levels , regardless of the indices used . -
pubmed
Leptin has been shown to modulate
deiodinase type 1 ( D1 ) and type 2 ( D2 ) enzymes responsible for
thyroxine ( T4 ) to
triiodothyronine ( T3 ) conversion . -
pubmed
The AN - HDL treated cells showed an increased production of
NO at 3 h versus control -
HDL treated cells and a decreased production at 24 h. Regarding
LDL , a significant increase of
NO production was obtained both at 3 and 24 h. The
AN -
HDL and AN - LDL treated cells showed an increased production of
peroxynitrite both at 3 and 24 h compared to control lipoproteins . -
pubmed
Densitometric analysis of bands indicated that both
iNOS and
nNOS protein levels were significantly higher in the cells incubated with
AN lipoproteins compared to cells incubated with control lipoproteins both at 3 and 24 h. Although the pathogenesis of
AN remains uncertain , evidence exists that modifications to the lipoprotein profile and
cholesterol , structural alterations of phospholipids and integral constituents of myelin and synaptosomes may be related to
psychotic disorders and body image distortion common to
AN . -
pubmed
The multivariate analysis for biochemical
relapse - free survival revealed that
prostate - specific antigen ( relative risk , 1.002 ; 95 % CI , 1.001 - 1.003 ; P = 0.0041 ) , Gleason score ( relative risk , 1.166 ; 95 % CI , 1.046 - 1.302 ; P = 0.0055 ) , T classification ( relative risk , 2.897 ; 95 % CI , 1.999 - 4.230 ; P = 0.0000 ) , pelvic irradiation ( relative risk , 2.042 ; 95 % CI , 1.328 - 3.273 ; P = 0.0008 ) , and
androgen abletion ( relative risk , 0.321 ; 95 % CI , 0.240 - 0.427 ; P = 0.0000 ) were significant prognostic factors . -
pubmed
Physical nature of intermolecular interactions within
cAMP - dependent protein kinase active site : differential transition state stabilization in
phosphoryl transfer reaction . -
pubmed
Differential transition state stabilization ( DTSS ) methodology has been applied herein to the
phosphoryl transfer reaction catalyzed by
cAMP - dependent protein kinase (
PKA ) . -
pubmed
The
MP2 results correlate well with the available experimental data and theoretical findings indicating that
Lys72 ,
Asp166 , and the two
magnesium ions contribute -22.7 , -13.3 , -32.4 , and -15.2 kcal / mol to differential transition state stabilization , respectively . -
pubmed
Using scanning tunneling microscopy (
STM ) and density functional theory simulations , we have studied the diffusion of
alkoxy species formed by the dissociation of
alcohols on bridge - bonded
oxygen (
BBO ) vacancies (
BBO ( V ) 's ) on
TiO2 ( 110 ) . -
pubmed
At elevated temperatures ( & gt;or=400 K ) the sequential isothermal
STM images show that mobile
BBO ( V ) 's mediate the diffusion of
alkoxy species by providing space for
alkyl - group - bearing
BBO atom to diffuse into . -
pubmed
TEM images and thermogravimetric analysis reveal that the microemulsion interface and the
PEG400 agent have a synergistic effect on the morphology and crystalline size transition of
ZnO nanostructures . -
pubmed
Immersion experiments in a simulated body fluid (
SBF ) were carried out to examine the
Ca - P phase forming ability of the modified surfaces . -
pubmed
Ectopic expression of
beta - catenin also decreased the expression of
GLI1 in the
butyrate treated
cancer cells . -
pubmed
The peak temperature of the temperature - programmed desorption profiles of
toluene from the CC microspheres was higher than that from granular activated
carbon (
GAC ) with numerous micropores , indicating that
toluene is adsorbed more strongly on CC microspheres than on
GAC . -
pubmed
P2Y12 receptor - mediated
integrin - beta1 activation regulates microglial process extension induced by
ATP . -
pubmed
Microglia rapidly extend their processes toward the damaged areas in response to stimulation of the
metabotropic ATP receptor P2Y ( 12 ) by
ATP released from damaged tissue . -
pubmed
In this study , we showed that
ATP increased microglial adhesion to
collagen gel , and that the
ATP - induced process extension and increase in microglial adhesion were inhibited by
integrin blocking peptides , RGD , and a functional blocking antibody against
integrin - beta1 . -
pubmed
An immunoprecipitation analysis with an antibody against the active form of
integrin - beta1 showed that
P2Y ( 12 ) mediated the
integrin - beta1 activation by
ATP . -
pubmed
These findings indicate that
ATP induces the
integrin - beta1 activation in microglia through
P2Y ( 12 ) and suggest that the
integrin - beta1 activation is involved in the directional process extension by microglia in brain tissue . -
pubmed
The decapeptide
gonadotropin - releasing hormone (
GnRH ) is the primary factor responsible for the hypothalamic control of
gonadotropin secretion . -
pubmed
GnIH acts on the pituitary and on GnRH neurons in the hypothalamus via a novel
G protein - coupled receptor (
GPR147 ) . -
pubmed
GnIH decreases
gonadotropin synthesis and release , inhibiting gonadal development and maintenance . -
pubmed
Melatonin stimulates the expression and release of
GnIH via
melatonin receptors expressed by
GnIH neurons . -
pubmed
Data indicate that
serine /
threonine protein kinase
Akt (
Akt ) signaling cooperates with
Wingless (
Wnt ) to activate
beta - catenin in intestinal stem and progenitor cells through phosphorylation at
Ser552 (
P - beta - catenin(552 ) ) . -
pubmed
The midpoint
cytotoxicity values for a 24 - hr exposure to
dichlone was about 0.02 mm when evaluated with the
neutral red ,
acid phosphatase , and
XTT tetrazolium assays . -
pubmed
Lactic acid dehydrogenase leakage , after a 4 - hr exposure , occurred initially at 0.05 mm
dichlone . -
pubmed
We first identified that HLA - B*1502 is strongly associated with
carbamazepine (
CBZ)-induced
SJS /
TEN and
HLA - B*5801 with
allopurinol -
SJS /
TEN in Han Chinese . -
pubmed
We used
MG-132 treatment of
HBE cells as an in vitro model to standardize the efficacy of
salubrinal ( inhibitor of
eIF2alpha de - phosphorylation ) in controlling the accumulation of ubiquitinated proteins and the
NFkappaB levels . -
pubmed
Moreover , our data suggest the therapeutic efficacy of
salubrinal in restraining
NFkappaB mediated
inflammation in the adult or older subjects . -
pubmed
The elevated
cdc2 had inactivating phosphorylation on
Thr(14 ) or
Tyr(15 ) , and possibly both , due to an increase in the responsible
Wee1 and
Myt1 kinases . -
pubmed
In cells that harbored cytoplasmic
cyclin B1 or
cdc2 , there was also an accumulation of the
phosphatase - inactive
cdc25C phosphorylated on
Ser(216 ) , unable to activate
cdc2 . -
pubmed
Moreover ,
E7 expression induced elevated expression of phosphorylated
ATM (
Ser(1981 ) ) and the downstream phosphorylated
Chk1 ,
Chk2 , and
JNKs ,
kinases known to inactivate
cdc25C. -
pubmed
A single percutaneous IT injection of
BoNT - B 0.5 U at 2 or 5 days prior to IPLT
formalin reduced
NK1 - R internalization and
C - Fos expression . -
pubmed
IT
BoNT - B also produced a corresponding reduction in phase 2 of
formalin - evoked flinching behavior for over 30 days after IT injection . -
pubmed
Romidepsin : a novel
histone deacetylase inhibitor for
cancer . -
pubmed
INTRODUCTION :
Romidepsin is a novel
histone deacetylase (
HDAC ) inhibitor , with a recent approval for treatment of
cutaneous T - cell lymphoma (
CTCL ) . -
pubmed
In addition , the Phase
II trial in
CTCL leading to FDA approval of
romidepsin is reviewed in detail . -
pubmed
Literature search was performed using PubMed ; keywords and concepts used included
romidepsin , T - cell
lymphoma and
HDAC inhibitors . -
pubmed
EXPERT OPINION :
Romidepsin is a potent
HDAC inhibitor with demonstrable activity in
T - cell lymphoma . -
pubmed
PURPOSE : Knowing the glycolytic phenotype of
cancers is important for the appropriate use of
F-18 FDG PET /
CT imaging . -
pubmed
The
F-18 FDG PET /
CT uptake was determined as maximum standard uptake value ( SUVmax ) at the pathologically confirmed site of disease or in the most active lesion . -
pubmed
SmCI is a 165 - residue glycoprotein with a molecular mass of 19.69 kDa ( mass spectrometry ) and 18
cysteine residues forming nine
disulfide bonds . -
pubmed
SmCI is the first proteinaceous inhibitor of
metallocarboxypeptidases able to act as well on another mechanistic class of
proteases (
serine - type ) and is the first of this kind identified in nature . -
pubmed
These
PAHs changed the studied gene transcriptions differently , but not significantly , except for
CYP1A , which was induced only by
RET . -
pubmed
MRSA cell counts on the surface of the non - film - coated control sheet were high ( 6,600 - 11,000 colony forming units ( cfu ) ) , but those on the
copper film were considerably lower ( 20 - 130 cfu ) . -
pubmed
Use of metallic
copper on the bed sheets of patients who are likely to be a source of
MRSA contamination may help to prevent the spread of
MRSA contamination in hospital wards . -
pubmed
We analyzed the amounts of some noteworthy proteins in these two samples including
lectin ,
superoxide dismutase ,
glycoside hydrolase and proteins involved in
cordycepin metabolism , providing important information for further protein studies . -
pubmed
This study investigated the extent of
carbon capture in a wet - dumped ash dam and the mineralogical changes promoting
CO2 capture , comparing this natural phenomenon with accelerated ex - situ mineral carbonation of fresh fly ash (
FA ) . -
pubmed
In view of the predicted frequencies and relative clinical significance of both private
antigens and high - prevalence
antigens absent , the most relevant assays for individuals of African descent in a transfusion setting are for 1 ) partial
RH2 in the patient and 2 ) RH54 ( DAK ) in the donor . -
pubmed
Experimental measurements indicate that , when compared to the commonly known
face - centered cubic ( 3C ) form of
silver , the
4H - Ag form shows a 130 - fold higher , strongly anisotropic , in - plane resistivity and a much lower optical reflectance with a pronounced surface plasmon contribution that imparts a distinctive golden hue to the material . -
pubmed
The pressure swing adsorption (
PSA ) , physical absorption ( Selexol ) , and chemical looping combustion (
CLC ) technologies were separately analyzed for processes with
CO2 capture . -
pubmed
The results show that , when only electricity production in considered , the case using
CLC technology has an electrical efficiency 1.3 % and 2.3 % higher than the
PSA and
Selexol based cases , respectively . -
pubmed
The
CLC based process achieves an overall
CO2 capture efficiency of 99.9 % in contrast to 89.9 % for
PSA and 93.5 % for
Selexol based processes . -
pubmed
The overall efficiency of the
CLC case for combined electricity and
H2 production is marginally higher ( by 0.3 % ) than
Selexol and lower ( by 0.6 % ) than PSA cases . -
pubmed
These
plasmanylinositols respond acutely to stimulation of cells with chemoattractants , and their levels are regulated by
PIPKs ,
PI3Ks and
PTEN . -
pubmed
( R )- 3 - amino-1 -(( 3aS,7aS )- octahydro-1H - indol-1 - yl )- 4 -( 2,4,5 - trifluorophenyl ) butan-1 - one derivatives as potent inhibitors of
dipeptidyl peptidase-4 : design , synthesis , biological evaluation , and molecular modeling . -
pubmed
A series of
( R )- 3 - amino-1 -(( 3aS,7aS )- octahydro-1H - indol-1 - yl )- 4 -( 2,4,5 - trifluorophenyl ) butan-1 - one derivatives was designed , synthesized , and evaluated as novel inhibitors of
dipeptidyl peptidase-4 (
DPP-4 ) for the treatment of
type 2 diabetes . -
pubmed
Thus , a highly sensitive and selective ECL probe for the recognition of
Co ( 2 + ) was developed with a stable response over a concentration range of 0.9 muM to
6.3 muM and a detection limit as low as 21 nM. -
pubmed
Moreover , the taxonomic analysis based on high through - put
16S rRNA gene sequencing revealed that the high abundance of denitrifying bacteria and
nitrite - oxidizing bacteria ( NOB ) was responsible for low
nitrite accumulations and consequent low
N2O emissions . -
pubmed
The meta - analysis also revealed decreased
CK - MB ,
cTnI and
MDA , and increased content of
SOD . -
pubmed
Vpac2a is activated exclusively by peptide
histidine isoleucine (
PHI ) , which suggests that
Pac1a mediates the melanin aggregating effect of
PACAP on melanophores . -
pubmed
Efficacy of
Lapatinib in Therapy - Resistant
HER2 - Positive Circulating
Tumor Cells in Metastatic
Breast Cancer . -
pubmed
BACKGROUND : To evaluate the efficacy of
lapatinib , a dual
EGFR and
HER2 tyrosine kinase inhibitor , in therapy - resistant
HER2 - positive
CTCs in metastatic
breast cancer (
MBC ) . -
pubmed
Multifactorial resistance to
aminopeptidase inhibitor prodrug
CHR2863 in
myeloid leukemia cells : down - regulation of
carboxylesterase 1 , drug sequestration in lipid droplets and pro - survival activation
ERK /
Akt /
mTOR . -
pubmed
Here we identified the molecular basis of acquired resistance to
CHR2863 , an orally available hydrophobic aminopeptidase inhibitor prodrug with an
esterase - sensitive motif , in
myeloid leukemia cells . -
pubmed
CHR2863 enters cells by diffusion and is retained therein upon
esterase activity - mediated conversion to its hydrophilic active metabolite drug
CHR6768 , thereby exerting
amino acid depletion . -
pubmed
Remarkably , the latter feature coincided with a gain of sensitivity to the
mTOR inhibitor
rapamycin . -
pubmed
The electronic structures show that the large
Li adsorption energy and fast diffusion ability of the
P / G origin from the interfacial synergy effect . -
pubmed
The scaffolding and hub protein
postsynaptic density protein-95 (
PSD-95 ) is a major element of central chemical synapses and interacts with
glutamate receptors , cell adhesion molecules , and cytoskeletal elements . -
pubmed
Pharmacological activities are mainly mediated via
opioid receptors as well as neuronal
Ca2 + channels , expression of
cAMP and
CREB protein and via descending monoaminergic system . -
pubmed
Osimertinib (
AZD9291 ) , a Mutant - Selective
EGFR Inhibitor , Reverses
ABCB1 - Mediated Drug Resistance in
Cancer Cells . -
pubmed
In this study , we determine whether
osimertinib , a novel selective , irreversible
EGFR (
epidermal growth factor receptor ) TKI , could reverse
ABC transporter - mediated MDR . -
pubmed
The results showed that , at non - toxic concentrations ,
osimertinib significantly sensitized both
ABCB1 - transfected and drug - selected cell lines to substrate anticancer drugs
colchicine ,
paclitaxel , and
vincristine . -
pubmed
Osimertinib significantly increased the accumulation of
[ 3H ]- paclitaxel in
ABCB1 overexpressing cells by blocking the efflux function of
ABCB1 transporter . -
pubmed
In contrast , no significant alteration in the expression levels and localization pattern of
ABCB1 was observed when
ABCB1 overexpressing cells were exposed to 0.3 microM
osimertinib for 72 h. In addition ,
ATPase assay showed
osimertinib stimulated
ABCB1 ATPase activity . -
pubmed
Taken together , our findings suggest that
osimertinib , a clinically - approved third - generation
EGFR TKI , can reverse
ABCB1 - mediated MDR , which supports the combination therapy with
osimertinib and
ABCB1 substrates may potentially be a novel therapeutic stategy in
ABCB1 - positive
drug resistant cancers . -
pubmed
Hemodilution was produced by mixing the blood with 40 %
Tris / saline solution , fibrinolysis by treating the blood with 160 ng / mL
tPA ,
acidosis by adding 1.2 mg / mL
lactic acid achieving pH 7.0 to 7.1 , and
hypothermia by running the assay at 31 C. Intact blood tested at 37 C served as control . -
pubmed
In 2014 ( 2015 , 2016 ) , 7 % ( 14 % , 19 % ) , 17 % ( 27 % , 34 % ) , 51 % ( 67 % , 70 % ) and 88 % ( 97 % , 98 % ) of the population in China lived in areas that meet the level of annual PM2.5 , PM10 ,
NO2 , and
SO2 standard metrics from Chinese Ambient Air Quality Standards - Grade
II . -
pubmed
Here , we characterized the liver macrophage subsets during
methionine /
choline deficient (
MCD ) diet - induced
NASH and recovery . -
pubmed
IL - MG was synthesized by a simple and time - saving one -
pot strategy where the synthesis of magnetic
Fe3O4 , the modification with an IL , and the reduction of
graphene oxide to
graphene were conducted at the same time . -
pubmed
Moreover , 1064 AG patients had significantly higher frequencies of
microcytosis ,
macrocytosis ,
hemoglobin ,
iron ,
vitamin B12 , and
folic acid deficiencies ,
hyperhomocysteinemia , and serum
GPCA positivity than 532 healthy control subjects ( all P - values & lt ; 0.005 ) . -
pubmed
OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low
Nitrogen and
Phosphate . -
pubmed
Auxin ,
nitric oxide (
NO ) , and
strigolactones ( SLs ) are important regulators of root growth under low -
nitrogen and -
phosphate ( LN and
LP ) conditions . -
pubmed
qRT - PCR analysis of
OsPIN family genes showed that the expression of
OsPIN1b is most abundant in root tip and is significantly downregulated by LN ,
LP ,
sodium nitroprusside (
SNP ,
NO donor ) , and
GR24 ( analogue of SLs ) treatments . -
pubmed
Seminal roots in
ospin1b mutants were shorter than those of the wild type ; and the seminal root ,
[ 3H ] IAA transport , and
IAA concentration responses to LN ,
LP ,
SNP , and
GR24 application were attenuated in
ospin1b-1 mutants . -
pubmed
Between baseline and month 6 , mean serum
bicarbonate increased from 23.1 to 24.1 mmol / L ( P = 0.01 ) , mean
serum albumin increased from 36.8 to 37.8 g / L ( P = 0.03 ) , mean
serum C - reactive protein increased from 7.3 to 12.4 mg / L ( P = 0.05 ) , and mean serum
potassium decreased from 4.80 to 4.59 mmol / L ( P = 0.01 ) . -
pubmed
Despite showing clinical activity in
BRAF - mutant
melanoma , the
MEK inhibitor ( MEKi )
trametinib has failed to show clinical benefit in
KRAS - mutant
colorectal cancer . -
pubmed
The bromodomain inhibitor
JQ1 suppressed interferon - stimulated gene (
ISG ) expression and in combination with
MEK inhibitors displayed synergistic effects and induced apoptosis in MEKi - resistant
colorectal cancer cell lines . -
pubmed
ISG expression was confirmed in patient - derived organoid models , which displayed resistance to
trametinib and were resensitized by
JQ1 co - treatment . -
pubmed
Since the
PGE2 receptor has four subtypes ,
EP1 - EP4 , this study was aimed to challenge the hypothesis that at least one of the four subtypes is responsible for the pathogenesis of
dioxin - induced
hydronephrosis . -
pubmed
To expose the pups to
2,3,7,8 - tetrachlorodibenzo - p - dioxin (
TCDD ) via lactation , the dams were administered
TCDD at an oral dose of 20 mug / kg on
PND 1 . -
pubmed
We found that the incidence of
hydronephrosis was 80 % in the
EP1+/+ group , but was markedly reduced to 28.6 % in the
EP1-/- group despite the fact that
PGE2 concentration in the urine was similarly increased in the both groups . -
pubmed
With regard to
EP4 , the incidence of
hydronephrosis in vehicle ( saline)-treated groups and
ONO - treated was 88.9 % and 100 % , respectively . -
pubmed
Tyrosinase based amperometric biosensor for determination of
tyramine in fermented food and beverages with gold nanoparticle doped
poly ( 8 - anilino-1 - naphthalene sulphonic acid ) modified electrode . -
pubmed
The biosensor architecture is based on
tyrosinase (
Tyrase ) immobilization on
glassy carbon electrode modified by a nanocomposite consisting of gold nanoparticles ( AuNP ) synthesized by a green method and
poly ( 8 - anilino-1 - naphthalene sulphonic acid ) modified
glassy carbon electrode . -
pubmed
OBJECTIVES : To determine the proportion of
rifampicin - resistant
tuberculosis (
RR - TB ) patients who accessed second - line drug susceptibility testing ( SL -
DST ) results following introduction of the Hain technology in southern provinces , Zimbabwe . -
pubmed
We , for the first time , prospectively evaluated the healed status in
TB spine by
fluorodeoxyglucose - positron emission tomography / computed tomography (
FDG - PET /
CT ) and contrast magnetic resonance imaging ( MRI ) with the objective to define end - point of treatment in
TB spine . -
pubmed
ATT was stopped on contrast MRI - based healing or absence of
FDG uptake on PET -
CT . -
pubmed
FDG - PET /
CT showed healed bone lesion in 28/28 ( 100 % ) and on MRI 13/19 ( 68.42 % ) , respectively . -
pubmed
Conclusions :
FDG - PET /
CT is more useful to demonstrate the healed status than MRI and is the only imaging to demonstrate healed status when MRI could not be performed due to metallic implants . -
pubmed
Oxylipins include hundreds of structurally and stereochemically distinct lipid species which
today are most commonly analyzed by ( ultra ) high performance liquid chromatography - mass spectrometry based ( ( U)HPLC -
MS ) methods . -
pubmed
The meta - analysis showed significantly better fasting blood
glucose and
hemoglobin A1c (
HbA1c ) level . -
pubmed
In secondary analyses , there was a remarkable reduction in total
cholesterol and
low - density lipoprotein postintervention . -
pubmed
IMPACT : Existing reviews are limited to narrative synthesis , lacking critical appraisal , heterogenous outcomes , nor any report of fasting blood
glucose and HbA1c . -
pubmed
Then , three electrodes of
ITO /
VO2(M)/
Au were prepared : electrode ( I ) , electrode (
II ) , and electrode ( III ) at annealing temperatures of 550 , 650 , and 750 C , respectively . -
pubmed
Independent predictors of
PMV included age , intraoperative maximum vasoactive - inotropic score , minimal temperature during cardiopulmonary bypass , postoperative
prothrombin time ,
alkaline phosphatase and total
bilirubin levels , and postoperative fluid balance . -
pubmed
CONCLUSIONS : Age at surgery , maximal vasoactive - inotropic score and minimal temperature during cardiopulmonary bypass , postoperative
prothrombin time ,
alkaline phosphatase and total
bilirubin levels , and postoperative fluid balance were demonstrated to be independent predictors of
PMV . -
pubmed
Accordingly , IM -
MS experiments performed in
Ag+-doped
methanol revealed the presence of both
( + ) - and ( -)- alpha - bisabolol in the EO , while molecular simulations provided the structures of the two
alpha - bisabolol enantiomer
silver ( I ) adducts . -
pubmed
Methods : Two adult patients with
type 1 diabetes (
T1D ): one new onset and the other one with established
T1D are presented where telehealth facilitated by Clarity Software and the " Share " feature with the use of Dexcom
G6 continuous
glucose monitoring ( CGM ) for management of
diabetic ketosis and
hyperglycemia . -
pubmed
Shared
glucose data through CGM facilitated frequent
insulin dose adjustments , increased fluid and
carbohydrate intake , and prevented hospital admissions in both cases . -
pubmed
The reduction in the gene expression levels of
Opa1 and
Drp1 was also reversed by
MA-5 , suggesting the modification of the fusion / fission process . -
pubmed
At the end of 60 days on these diets , blood
glucose , total blood
cholesterol ,
triglycerides (
TGs ) , and
high density lipoprotein (
HDL ) were quantitated . -
pubmed
MATERIALS AND METHODS : The
cytotoxic activity of the compound ( at 0.1 - 20.0 mug / mL ) on the cells was determined by Real - Time Cell Analysis ( RTCA ) system for 24 h. The estimation of cell cycle arrest , reactive
oxygen species ( ROS ) production , reduction of mitochondrial membrane potential (
MMP ) , and
caspases-3/
7/9 activity in the HeLa cells treated with the compound was done by flow cytometry and luminometric technique . -
pubmed
Demographic data and imaging data of fundus photography , fundus autofluorescence (
FAF ) , confocal blue - light reflectance ( CBR ) ,
fluorescein angiography ( FAG ) , and optical coherence tomography ( OCT ) were collected . -
pubmed
ATPase activity of
cardiac myosin was stimulated as the
Ca2 + concentration in the incubation medium increased . -
pubmed
No difference was found in
Ca2 + -activation , salt sensitivity or
ATPase activity of unreacted and
sulphydrylmodified cardiac myosins from euthyroid or
hyperthyroid groups . -
pubmed
The
TCN was also phosphorylated in a cellfree extract containing
adenosine kinase activity at about the same rate as was
adenosine , but not further phosphorylation of the analogue MP occurred . -
pubmed
Added protein reduced the effectiveness of
Cu ( II ) as a catalyst of the reaction between
ascorbate and
MNNG , which suggested that the microsomal
protein necessary to activate
DMN , may reduce the concentration of free
Cu ( II ) and thereby lower its catalytic efficiency . -
pubmed
Serum
Ca was 1.35 mmol / l , P = 0.99 mmol / l with high
alkaline phosphatases ( A.P. ) -
pubmed
Serum
Cu was low = 3.14 mumol / l and serum immunoreactive
parathormone (
iPTH ) level was elevated : 520 mulEq / ml ( N less than or equal to 100 ) . -
pubmed
Administration of
vitamin D2 ( 15 mg ) induced an immediate normalization of serum
Ca , normal serum
iPTH ( 68 mulEq / ml ) in one month , normal X - rays in two months and normal A.P. -
pubmed
We have made optical detection of magnetic resonance ( ODMR ) measurements on the enzyme
alpha - chymotrypsin , as well as on its complex with the dye ,
proflavin . -
pubmed
The luminescence decay kinetics of the complex indicates that nontrivial triplet - singlet transfer originates from several ( at least three )
tryptophan residues of the
enzyme . -
pubmed
The method is very simple as sample preparation is minimal , and the isocratic elution of the
C18 column with pure
methanol does not necessitate a sophisticated instrumental set - up . -
pubmed
cruzi causes in the expression of
TcR ,
CD4 ,
CD8 and
IL-2R -- all
molecules playing important roles in lymphocyte activation -- could contribute to the development of the immunosuppression observed during the acute phase of
Chagas ' disease . -
pubmed
All of them are non - enzymatically induced by 0.1 mM
calcium ion , which is the ultimate concentration of sarcoplasmic
calcium ion in
post mortem muscles . -
pubmed
Transnasal
butorphanol : a new method for
pain relief in
post - cesarean section
pain . -
pubmed
It is concluded that transnasal
butorphanol represents a safe and effective alternative to injectable
butorphanol for
post - cesarean section
pain and offers a better and longer duration of analgesia compared to
IV butorphanol . -
pubmed
The optimum dose seems to be 2 mg
TN butorphanol and it is tolerated better when divided into 1 mg increments , given 1 h apart . -
pubmed
The effect of
ATP is apparently mediated by the activation of
cytosolic protein kinases and requires the influx of
Ca2 + through the plasma membrane . -
pubmed
All sera were tested for total
immunoglobulin (
Ig ) levels ;
IgG ,
IgM , and
IgA isotypes of autoantibodies to six
phospholipids ; total
histone and four
histone subfractions ; and four polynucleotides . -
pubmed
Only a few autoantibodies were increased in early pregnancy , including
IgG antiphosphatidylinositol and
H 2B ,
IgM antiphosphatidylinositol and
phosphatidic acid , and
IgA antiphosphatidylinositol and H 4 . -
pubmed
Amino acid substitutions are nonrandomly distributed throughout the
apolipoprotein A - I primary structure . -
pubmed
These substitutions are the result of transversions of
cytosines contained within stretches of at least 5 consecutive
cytosines in the
apoA - I gene . -
pubmed
The observed hypervariability of the
apoA - I amino terminus , therefore , might be caused by a hot
spot for mutation formed by the 7 subsequent
cytosines in codons 3 , 4 , and 5 . -
pubmed
The hypervariability of the
apoA - I alpha - helical domain might therefore be caused by
CpG dinucleotides predominantly occurring in codons 120 - 208 of
apoA - I ( 82 out of 125 ) . -
pubmed
The isosteric
CH ---- N substitution in the class of
2 - arylpenems results in improved antibacterial activity , with retention of the favorable characteristic of stability towards
renal dehydropeptidase . -
pubmed
The aggregational state and conformation of the
protein were affected by ( 1 ) the method of coat
protein isolation (
phenol extraction vs
cholate isolation ) , ( 2 ) the nature of amphiphiles used ( variation in phospholipid headgroups and
acyl chains ) , and ( 3 ) the ratio of amphiphiles and protein . -
pubmed
Under all conditions ,
phenol - extracted
coat protein was in a predominantly beta - structure and in a highly aggregated polymeric form . -
pubmed
Cholate - isolated
coat protein was initially oligomeric and contained a substantial amount of alpha - helix . -
pubmed
This effect was observed upon uptake in phospholipids at low lipid to protein molar ratios ( L / P ratios ) and with
phosphatidylcholines (
PC ) and
phosphatidic acids (
PA ) containing saturated
acyl chains . -
pubmed
After reconstitution in phospholipids with unsaturated
acyl chains and with
phosphatidylglycerols ( PG ) at high L / P ratios , the original alpha - helix - containing state of the
coat protein was maintained . -
pubmed
Ontogenetic changes in the
cyclic adenosine 3 ' , 5 '- monophosphate - stimulatable phosphorylation of cat visual cortex proteins , particularly of
microtubule - associated protein 2 (
MAP 2 ): effects of normal and dark rearing and of the exposure to light . -
pubmed
In vitro phosphorylation was assayed with and without
cAMP and the
cAMP - dependent protein kinase , and the phosphoproteins separated by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis were counted for
32P incorporated from
[ gamma-32P ] ATP . -
pubmed
By contrast , the
cAMP - stimulatable phosphorylation of
microtubule - associated protein 2 (
MAP 2 ) , which under normal rearing conditions increases from birth to the second month , is strongly modulated by the presence of light in the environment . -
pubmed
The
cAMP - dependent phosphorylation of
MAP 2 ( and its dephosphorylation ) may be an important factor for determining the state of plasticity in the
CP through its affecting the dendritic cytoskeletal organization involving
tubulin and
actin . -
pubmed
Needle biopsy evaluation of
class II major histocompatibility complex antigen expression for the differential diagnosis of
cyclosporine nephrotoxicity from kidney graft rejection . -
pubmed
2 - Deoxyglucose uptake was about 95 % depressed in
insulin - treated , but not in ' basal ' , cells preincubated with
epinephrine plus
adenosine deaminase for 60 min in broad agreement with Green 's report . -
pubmed
In similarly incubated cells , transport of
3 - O - methylglucose , a
sugar analogue not phosphorylated in the adipocytes , was not affected by
catecholamine plus
adenosine deaminase . -
pubmed
However , a decrease in transport of about 60 % was observed both in the absence and the presence of
insulin when the
albumin concentration was high enough and the cell concentration low enough to prevent accumulation of free
fatty acids in the medium . -
pubmed
In addition , the
insulin sensitivity with regard to
hexose transport was markedly reduced . -
pubmed
Transport was approximately doubled in cells incubated with 5 microM
epinephrine in the absence of
adenosine deaminase . -
pubmed
Thus ,
epinephrine at a high concentration stimulates
hexose transport in the absence of
adenosine deaminase ( presence of
adenosine ) whereas it inhibits both basal and
insulin - stimulated transport in the presence of
adenosine deaminase ( absence of
adenosine ) . -
pubmed
Serial
prolactin growth hormone ,
insulin , blood
sugar ,
cortisol ,
glucagon , thyrotropin stimulating hormone ,
thyroxine , and
triiodothyronine were obtained by RIA . -
pubmed
Requirement of substrate for
adenosine 5 '- monophosphate binding to
liver fructose 1,6 - diphosphatase . -
pubmed
Breakage of DNA in
rec+ and
Rec- bacteria by disintegration of
radiophosphorus atoms in DNA and possible cause of pleiotropic effects of
RecA mutation . -
pubmed
Glutamine showed increased rates of uptake to the sensorimotor cortex during stimulation periods , suggesting an accelerated breakdown via
glutaminase . -
pubmed
Biochemical studies showed only trivial increases in levels of
glutamic - pyruvic transaminase (
GPT ) ,
glutamic - oxalacetic transaminase (
GOT ) , and moderate but temporary increases in
isocitrate dehydrogenase (
ICD ) after
CCl4 alone . -
pubmed
[
LH - RH agonist
buserelin ] . -
pubmed
The regulation of
acetoacetyl - CoA synthetase activity by modulators of
cholesterol synthesis in vivo and the utilization of
acetoacetate for cholesterogenesis . -
pubmed
Acetoacetyl - CoA synthetase activity was depressed by feeding
cholesterol or
mevalonate administration and was enhanced by the addition to the diet of
mevinolin and/or
cholestyramine . -
pubmed
The evidence suggests that the regulation of
acetoacetyl - CoA synthetase activity and the utilization of
acetoacetate for lipogenesis is closely linked to the regulation of cholesterogenesis . -
pubmed
Turnover of
low density lipoproteins during inhibition of
cholesterol absorption by
neomycin . -
pubmed
During treatment with
neomycin , the plasma total
cholesterol fell by an average of 20 % ,
low density lipoproteins (
LDL ) fell by 25 % , and
high density lipoproteins , by 16 % . -
pubmed
Neomycin decreased synthesis of
apoLDL by 28 % . -
pubmed
Differences between
HbA and Chromatium high - spin
hemoprotein spectra can be assigned to either the effects of a covalent linkage of the
heme vinyls to the
protein matrix or alterations in the
heme - proximal ligand bonding interaction . -
pubmed
The behavior of
heme core - size sensitive modes and low - frequency modes in Chromatium high - spin
hemoprotein may be an indication of distortions in the
heme geometry of Chromatium high - spin hemoprotein relative to
HbA. -
pubmed
Re - chromatography of
CWI peak fractions on
SPS using
NaCl - gradient elution showed the presence of a 17 - kDa peptide (
p17 ) in fractions with low
CWI activity but strong
CWI immunosignal ( Weil and Rausch 1994 , Planta 193 , 430 - 437 ) . -
pubmed
When separating
CWI from
p17 by Concanavalin A ( Con A)-Sepharose chromatography , inhibition could be restored by incubating the inhibitor - containing fraction with inhibitor - free
CWI . -
pubmed
The presence of divalent metal ions (
Ca2 + ,
Mg2 + ,
Zn2 + ) during pre - incubation of
CWI with
p17 reduced
CWI inhibition substantially . -
pubmed
Also ,
sucrose protected CWI against inhibition by
p17 ( half - maximum protection at 1.3 mM ) . -
pubmed
When fractions containing
CWI and
p17 were analyzed by
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis (
SDS - PAGE ) and subsequent Western blotting a diffuse immunosignal of 86 - 90 kDa was observed ( in addition to the prominent CWI signal at 69 kDa). -
pubmed
The
pyridylcyanoguanidine pinacidil exerts its
hypotensive effect by opening
ATP - sensitive potassium channels ( K+ATP ) in vascular smooth muscle . -
pubmed
Methylation analysis and 1 and 2D NMR spectroscopy showed that the
K10 capsular polysaccharide has the structure [ formula : see text ] -
pubmed
Treatment of the cells with
L - DOPS increased the
NGF content in the L - M cell medium by approximately 3 - fold . -
pubmed
The antioxidants ,
ascorbic acid and
sodium pyrosulfite , completely prevented the stimulatory effect of
L - DOPS , and radical scavengers (
superoxide dismutase plus
catalase ) caused a significant partial inhibition of the response to
L - DOPS . -
pubmed
Quinone derivatives (
adrenochrome ,
4 - n - propyl-1,2 - benzoquinone ) , which are the oxidative products of the
catechol derivatives , increased the
NGF content in the medium , and their potency was greater than that of the
catechol derivatives themselves . -
pubmed
However ,
PMA significantly abolished these effects of
SSb2 in including proliferation inhibition and apoptosis induction . -
pubmed
Down - regulation of the
PKC activity may be involved in the effect of
SSb2 . -
pubmed
The proportion of sperm with active
acrosin were determined using the
biotinylated isocoumarin serine protease inhibitor ,
Bi - Aca - Aca - OMe - IC (
BIC ) . -
pubmed
The presence of bound inhibitor on sperm was then determined by secondary labeling with
avidin fluorescein conjugate . -
pubmed
Histamine induced significant
MGP secretion from both explants and isolated glands . -
pubmed
The inhibitory action of
SKF 91488 in airway explants was blocked by
indomethacin and was significantly reduced by a
prostanoid EP4 receptor antagonist (
AH23848B ) . -
pubmed
Similar
calcium , conductance , and capacitance responses could be activated by
2 - methylthio - ATP and
ADP , consistent with mediation by a
P2 type purinergic receptor . -
pubmed
These myriad responses to extracellular
ATP suggest that
P2 receptor - mediated signaling is important in brown adipocyte physiology and that sympathetic stimulation may normally activate purinergic as well as adrenergic pathways in brown fat . -
pubmed
Using a
DIG - labeled
aMAG antisense RNA as a probe , we have investigated the developmental and tissue - specific expression of this repair gene . -
pubmed
Antral biopsy samples were taken for
hematoxylin - eosin stain (
HE ) , rapid urease test , and culture and corpus samples for culture and HE . -
pubmed
This leader presents striking similarity to ' T - box ' leaders found upstream of many Gram - positive
aminoacyl - tRNA synthetase genes and some
amino acid biosynthesis operons , which are controlled by antitermination through interaction of the leader transcript with cognate uncharged
tRNA . -
pubmed
Surprisingly ,
RAD52(-/- ) cells were not hypersensitive to DNA damages induced by gamma - irradiation ,
methyl methanesulfonate , or
cis - platinum ( II ) diammine dichloride (
cisplatin ) . -
pubmed
A comparative
SAR study of
thrombin receptor derived non peptide mimetics : importance of
phenyl /
guanidino proximity for activity . -
pubmed
Synthetic
thrombin receptor peptides (
TRAPs ) comprising the first 5
amino acids ( SFLLR and SFLLR - NH2 ) of the new N - terminus tethered ligand of the
thrombin receptor that is generated by
thrombin 's proteolytic activity were found to cause full
platelet aggregation . -
pubmed
During the screening of novel
thrombin receptor derived non - peptide mimetics in the
platelet aggregation assay we found that
1 - phenylacetyl-4 -( 6 - guanidohexanoyl )- piperazine ( 1 ) and
1 -( 6 - guanidohexanoyl )- 4 -( phenylacetylamidomethyl )- piperidine ( 2 ) exerted in vitro antagonist activities ( 56 % and 40 % correspondingly ) as it is depicted by the
platelet aggregation assay . -
pubmed
Correlation between
polymerase chain reaction analysis of the
histidine biosynthesis operon , randomly amplified polymorphic DNA analysis and phenotypic characterization of dairy Lactococcus isolates . -
pubmed
The
O - oxime derivatives from
aldehyde reaction with
PFBHA were desorbed with
hexane , and quantified by capillary gas chromatography / mass spectrometry (
GC /
MS ) or gas chromatography / electron capture detection (
GC /
ECD ) . -
pubmed
Other data on
valeraldehyde and
acrolein have shown that the sampling constants were independent of
face velocity between 0.1 to 0.35 m / s ( 20 to 70 fpm ) , temperatures between 9 to 48 degrees C ,
RH between 3 to 79 percent , and intermittent sampling exposure pattern . -
pubmed
In all cases , the cells were present in
nodal sinuses and appeared as single cells or small clusters . -
pubmed
Parathion induced significant
depression of both
AChE and
LDH activities . -
pubmed
No significant assay interference was caused by a high concentration of
triglyceride ,
hemoglobin ,
bilirubin ,
uric acid , or
creatinine . -
pubmed
Neuronal apoptosis induced by pharmacological concentrations of
3 - hydroxykynurenine : characterization and protection by
dantrolene and
Bcl-2 overexpression . -
pubmed
In cerebellar granule cells , the
3 - HK neurotoxicity was unaffected by
xanthine oxidase inhibitors but markedly potentiated by
superoxide dismutase and its hemelike mimetic ,
MnTBAP [
manganese ( III ) tetrakis ( benzoic acid ) porphyrin chloride ] . -
pubmed
Catalase blocked
3 - HK neurotoxicity in the absence and presence of
superoxide dismutase or
MnTBAP . -
pubmed
Taken together , our results suggest that
neurotoxicity induced by pharmacological concentrations of
3 - HK in these cell types is mediated primarily by
H ( 2 ) O ( 2 ) , which is formed most likely by auto - oxidation of 3 -
HK in extracellular compartments . -
pubmed
The protection by
dantrolene was associated with a marked increase in the protein level of
Bcl-2 , a prominent antiapoptotic gene product . -
pubmed
Thus ,
dantrolene may elicit its neuroprotective effects by mechanisms involving up - regulation of the level and function of
Bcl-2 protein . -
pubmed
The pathophysiology of
IPS secondary to high - dose
busulfan - containing myeloablative regimens is not known , but cell - mediated immune reactions and release of
cytokines may contribute to the
lung injury . -
pubmed
Our data showed that the expression of
MRP1 - EGFP results in significantly decreased cellular accumulation of
tetramethylrhodamine ethyl ester (
TMRE ) and
daunorubicin , mildly decreased cellular accumulation of
mitoxantrone , and decreased nuclear accumulation of
doxorubicin . -
pubmed
Additionally ,
MRP1 - EGFP expression protected cells from the microtubule depolymerization caused by
vincristine and
colchicine , but not by
vinblastine . -
pubmed
In fact ,
angiotensinogen is up - regulated particularly by oral administration of
estrogen , whereas
renin ,
angiotensin - converting enzyme (
ACE ) , and
AT-1 receptor are down - regulated by the hormone . -
pubmed
While under well - defined experimental conditions the net effect of
estrogen appears to result in suppression of the
renin -
angiotensin system , the clinical situation may be more complex . -
pubmed
Nevertheless , the undisputable , profound biochemical changes in the
renin -
angiotensin system related to the
estrogen status allow speculation that such interaction explains some of the differences in the cardiovascular system of men and women . -
pubmed
Naloxone blocked
morphine - induced apoptosis suggesting involvement of an
opiate receptor mechanism . -
pubmed
Potent inhibition ( & gt;70 % ) of apoptosis by an inhibitor of
caspase-3 as well as co - localization of
active caspase-3 and DNA fragmentation in microglia or neurons treated with
morphine indicated that
caspase-3 is involved in the execution phase of
morphine - induced apoptosis . -
pubmed
In the presence of
Na2HPO4 /
NaH2PO4 , nanoparticles are agglutinated with
phosphate ions as a glue to grow in size up to 60 - 100 nm , as revealed by DLS as well as microscopy (
TEM and
AFM ) . -
pubmed
The
phosphate - induced agglutination processes can be followed by surface plasmon resonance (
SPR ) . -
pubmed
Amphiphile 2n is readily immobilized on the hydrophobized sensor chip of
SPR to give a closely packed monolayer with
oligosaccharide moieties exposed to bulk water . -
pubmed
The
ATP - supported treadmilling of
actin filaments , regulated by
Actin Depolymerizing Factor (
ADF /
cofilin ) ,
profilin and capping proteins provides the thermodynamic basis for sustained
actin - based movement . -
pubmed
Understanding the mechanism of
actin - based movement requires elucidation of the biochemical properties and mode of action of
Arp2 / 3 complex in filament branching , in particular the role of
ATP binding and hydrolysis in
Arp2 / 3 , and a physical analysis of the movement of functionalised particles . -
pubmed
The Pearson coefficient was also found to correlate significantly and negatively with the indices of vagal modulation and positively with the indices of sympatho - vagal balance and
rennin -
angiotensin -
aldosterone modulation in both normal controls and patients with
AMI . -
pubmed
CONCLUSIONS : Review indicated that ( 1 ) most published literature on fibre - based
posts took the form of laboratory analyses ; ( 2 ) evidence for
carbon - fibre
posts far exceeds that for
quartz - fibre
posts ; ( 3 ) laboratory evidence was contradictory and could not be used to inform practice reliably ; ( 4 ) few clinical studies have been carried out though these have suggested fibre based
posts may be clinically appropriate for restoration of the endodontically treated tooth ; and ( 5 ) controlled prospective clinical trials evaluating fibre - based
posts should be undertaken to inform use for clinical practice . -
pubmed
PMA , PA sup , and LPS increased
MUC5AC gene expression and
mucin protein production , effects that were prevented by pretreatment with
AG1478 , a selective inhibitor of
EGFR phosphorylation and by preincubation with an
EGFR - neutralizing Ab or with a
TGF - alpha - neutralizing Ab , implicating ligand (
TGF - alpha)-dependent
EGFR phosphorylation in mucin production . -
pubmed
Knockdown of
TACE inhibited
PMA- ,
PA sup- , and LPS - induced
TGF - alpha shedding ,
EGFR phosphorylation , and mucin production . -
pubmed
[ THE BEHAVIOR OF THE
INSULIN ACTIVITY OF THE BLOOD AFTER ORAL
GLUCOSE LOADING IN SUBJECTS WITH NORMAL METABOLISM AND IN DIABETICS ] . -
pubmed
Therefore , possible involvement of lysosomal
protease leakage caused by
hydroxyl radical formation in lysosomes ( possibly resulting in
mitochondrial membrane dysfunction ) is considered to play an important role in radiation - induced T cell apoptosis . -
pubmed
INTERVENTIONS : First , a morning blood sample was obtained to measure baseline
cortisol ,
corticotropin ,
interleukin-6 , and
tumor necrosis factor alpha . -
pubmed
RESULTS :
PARP inhibition with the
phenanthridinone - based
PARP inhibitor
PJ34 suppressed inflammatory cell migration . -
pubmed
The unusual primary structure of this component was confirmed by mass spectrometric and
amino acid sequence analyses of
cytoplasmic protein extracts . -
pubmed
Mechanism of
cardiac depression after
trauma -
hemorrhage : increased cardiomyocyte
IL-6 and effect of sex
steroids on
IL-6 regulation and cardiac function . -
pubmed
Cardiomyocytes were isolated and divided into two parts : one was used for measurements of intracellular
IL-6 levels using
fluorescein - activated cell sorting , and the other was used to isolate RNA to determine
IL-6 gene expression by quantitative real - time PCR . -
pubmed
Our approach , which requires no experimental input apart from knowledge of bulk crystal structures , is demonstrated for
palladium - copper alloys , which show nontrivial behavior due to the existence of
face - centered cubic and body - centered cubic crystal structures and have the potential to resist
sulfur poisoning . -
pubmed
In contrast , responses of the
diabetic ileum to the
Ca2 + channel agonist
Bay K8644 were greater than those of the controls , whilst the agonist failed to contract the colon . -
pubmed
Similarly , the
Ca2 + - ATPase inhibitors ,
thapsigargin and
cyclopiazonic acid , produced contractions which were greater in
diabetic ileal tissues . -
pubmed
Furthermore , knockdown of
p53 by pU6 -
p53 siRNA vector suggests the involvement of
MAP kinases in the process of
5 - aza - CdR - mediated activation of
p53 pathway to inhibit cell proliferation and induce apoptosis . -
pubmed
Finally , the comet or SCGE assay and methylation - sensitive restriction analysis demonstrated that
5 - aza - CdR induced
p53 and
p21Waf1 /
Cip1 expression as a consequence of DNA damage and independent of DNA demethylation . -
pubmed
Our findings suggest that
5 - aza - CdR induces anti - neoplastic activity primarily through the activation of
p53 pathway in response to DNA damage and subsequently leads to inhibition of cell proliferation as well as induction of apoptosis . -
pubmed
Therefore , our data indicate that
p53 status in
tumor cells may be critical for the clinical efficacy and toxicity of
5 - aza - CdR. -
pubmed
Resistance to
fluconazole and
flucytosine was observed infrequently ( 3.7 % and 3.0 % ) ;
16.2 % of isolates were resistant to
itraconazole ( MIC & gt ; or = 1 microg / ml ) . -
pubmed
krusei ( 50 % S -
DD , 41.7 % R , considered intrinsically
fluconazole resistant ) . -
pubmed
It is concluded that
IRL 1620 , an
ET ( B ) receptor agonist , does not alter
paclitaxel pharmacokinetics and can selectively augment the delivery of
paclitaxel to the
tumour tissue . -
pubmed
Breast cancer cell growth may be stimulated by
17beta - estradiol ( E2 ) or growth factors like
epidermal growth factor (
EGF ) . -
pubmed
Introduction of
ERalpha and treatment with
E2 decreased
EGF - induced
tyrosine phosphorylation of
STAT5b , basal and
EGF - induced
STAT5 - mediated transcription , and
EGF - stimulated DNA synthesis in these cells . -
pubmed
Phenserine reduces the production of the
amyloid precursor protein and
beta - amyloid in vitro . -
pubmed
Heat shock protein 70 as an indicator of early
lung injury caused by exposure to
arsenic . -
pubmed
Hsp70 protein , mRNA and reactive
oxygen species ( ROS ) generation were measured after exposing cells to
arsenic as markers of
injury . -
pubmed
Hsp70 protein expression showed significant 7.9 - fold and 31.5 - fold increase using Western blotting and ELISA assay , respectively , at a 50 microM
As ( III ) with a 12 h exposure and an 12 h recovery time . -
pubmed
As ( III )-induced
Hsp70 protein expression was inhibited significantly by
catalase and
NAC , indicating mediation of ROS in
Hsp70 expression . -
pubmed
These results suggest that
cellular injury by
arsenic is mediated through ROS generation resulting in the expression of
Hsp70 . -
pubmed
Freshly isolated UCB cells were cultured with
IL-3 ,
IL-5 and
GM - CSF in the presence or absence of a
gamma - secretase inhibitor
L-685,458 , and examined for the expression of
major basic protein (
MBP ) . -
pubmed
HbA(1c ) was 4.8 % and plasma
aldosterone :
renin ratios were elevated suggestive of increased
aldosterone secretion . -
pubmed
The adrenals were normal on
CT scanning and blood pressure responded dramatically to the addition of
spironolactone on day 212 ( 125/83 mmHg ) . -
pubmed
CONCLUSIONS : The prevalence of
primary hyperaldosteronism in the
hypertensive population , based on elevation of
plasma renin :
aldosterone ratio , is approximately 6 % . -
pubmed
Using the protein synthesis inhibitor
cycloheximide we demonstrate that
Hemolin up - regulation by 20E requires ongoing protein synthesis . -
pubmed
CONCLUSION : The most common pattern of injectate spread seen on MRI with both approaches to
PCB was spread within the body of the psoas muscle around the lumbar branches (
L2 - 4 ) , with cephalad spread to the lumbar nerve roots . -
pubmed
We show that the S - phase - specific
mec1 - 100 allele , like the
sgs1Delta mutation , partially destabilizes
DNA polymerases at stalled forks , yet combining the
mec1 - 100 and
sgs1Delta mutations leads to complete disassociation of the replisome , loss of
RPA , irreversible termination of
nucleotide incorporation , and compromised recovery from
hydroxyurea ( HU ) arrest . -
pubmed
Previous studies have shown that incubation of fibroblasts with
leptomycin B resulted in the accumulation of
galectin-3 in the nucleus , suggesting that the export of
galectin-3 from the nucleus may be mediated by the
CRM1 receptor . -
pubmed
Rev ( 1.4 )- galectin-3(240 - 255 ) exhibited nuclear export activity that was sensitive to inhibition by
leptomycin B. -
pubmed
Site - directed mutagenesis of
Leu247 and
Ile249 in the
galectin-3 nuclear export signal decreased nuclear export activity , consistent with the notion that these two positions correspond to the critical residues identified in the nuclear export signal of the
cAMP - dependent protein kinase inhibitor . -
pubmed
The nuclear export signal activity was also analyzed in the context of a full - length
galectin-3 fusion protein ;
galectin-3(1 - 263 ; L247A ) showed more nuclear localization than wild - type , implicating
Leu247 as critical to the function of the nuclear export signal . -
pubmed
These results indicate that residues 240 - 255 of the
galectin-3 polypeptide contain a
leucine - rich nuclear export signal that overlaps with the region ( residues 252 - 258 ) identified as important for nuclear localization . -
pubmed
The majority of the
adenosine 5 '- triphosphate (
ATP ) hydrolytic activity of purified vacuoles was associated with nonspecific
acid phosphatase and not with a transport
ATPase . -
pubmed
As judged by
acid phosphatase distribution and electron microscopy , the effective density of vacuoles in a
sucrose gradient was low ( less than 1.1 grams per cubic centimeter ) , although an unequivocal estimate of the vacuole or
tonoplast density was not possible from the experiments conducted . -
pubmed
Furthermore , low
O ( 2 ) prevented the rise in
acid phosphatase activities and this suppression was not reversed by the inclusion of 100 microliters per liter
ethylene in 2.5 %
O ( 2 ) atmosphere . -
pubmed
Low
O ( 2 ) suppressed the appearance of all
isoenzymes , and the addition of 500 microliters per liter
ethylene to the low
oxygen atmosphere did not reverse this effect . -
pubmed
Similarly , the decline in
pectin methylesterase that was observed in air - ripened fruits was prevented by 2.5 %
O ( 2 ) alone and in combination with 500 microliters per liter
ethylene . -
pubmed
In this virus - host system , the extents of
NF - kappaBeta - regulated gene expression and nuclear translocation were reduced in the presence of either
PD 98059 or
U0126 , two compounds capable of blocking
ERK1 and
ERK2 phosphorylation . -
pubmed
First , ectopic
M2L expression hampered
ERK2 phosphorylation induced by exposure to
phorbol myristate acetate . -
pubmed
Further , altered cell growth resulting from serum withdrawal and the induction of
caspase-3 activity by ultraviolet radiation were unchanged in the absence of
PPARbeta / delta expression and/or the presence of
GW0742 . -
pubmed
While no changes in the expression of mRNAs encoding cell cycle control proteins were found in response to
GW0742 , a significant decrease in the level of
ERK phosphorylation was observed . -
pubmed
KP-392 demonstrated increased
tumor necrosis and decreased nuclear
phospho - protein kinase /
Akt but did not change the levels of
phospho - extracellular signal - regulated kinase 1 / 2 . -
pubmed
Why does the disorder of R - pn and
rac - pn ligands in the quasi - one - dimensional
bromo - bridged NiIII complexes ,
[ Ni ( pn ) 2Br ] Br2 ( pn = 1,2 - diaminopropane ) afford similar
STM patterns ? -
pubmed
The disordered patterns of R- and
rac-1,2 - diaminopropane ( pn ) in quasi - one - dimensional
bromo - bridged
Ni ( III ) complexes ,
[ NiIII ( pn ) 2Br ] Br2 , have been investigated by single - crystal X - ray structure determination and scanning tunneling microscopy (
STM ) . -
pubmed
We have concluded that tunnel current from the
STM tip to metal ions are detected via
methyl groups of pn ligands . -
pubmed
This frameshift results in a truncated protein of 300
amino acids lacking the B30.2(SPRY ) domain , which we have named
TRIM5theta . -
pubmed
The activity of the
enzyme , like that of most
glycohydrolases , depends on a deprotonated
carboxylate ( nucleophile ) and a
protonated carboxylic acid for optimal activity . -
pubmed
The resulting pH - profile of k(cat)/K(m ) for the
beta - glucosidase - catalyzed hydrolysis of
p - nitrophenyl glucoside is characterized by a width at half height that is strongly sensitive to the nature and concentration of the salt . -
pubmed
There is a similar shift in the pK(a)s when the
LiCl concentration is increased to 1.0 M. The results of these salt - induced pK(a ) shifts rule out a significant contribution of reverse protonation to the catalytic efficiency of the
enzyme . -
pubmed
Here we show that
F - actin , detected by
dye - coupled phalloidin , is a major molecular component of the karyosome capsule in Neuroptera . -
pubmed
Vasodilation was measured by
venous occlusion plethysmography after intra - arterial infusions of
sodium nitroprusside and
acetylcholine and
insulin sensitivity by the intravenous
glucose tolerance test using the minimal model technique . -
pubmed
Lower feed intake , and therefore lower intake of the indispensable
amino acids , may have resulted in lower
pituitary GH and lower
IGF - I mRNA levels in muscle tissues . -
pubmed
In this study , we examined the role of
catecholamines via
alpha2 - adrenoreceptor in
acute murine colitis . -
pubmed
TH and
DBH are induced in LPMCs of inflamed colon , the evidence of
catecholamine synthesis during the process of
colitis . -
pubmed
RX821002 down - regulates the production of proinflammatory
cytokines from LPMCs , while
UK14304 leads to exacerbation of
colitis . -
pubmed
Together , our data show a critical role of
catecholamines via
alpha2 - adrenoreceptors in the progress of
acute colitis , and suggest that use of the
alpha2 - adrenoceptor antagonist represents a novel therapeutic approach for the management of
colitis . -
pubmed
Repetitive
alcohol ingestion and its resulting dependence is associated with false euphoria triggered by an inhibition of
glutamate receptors and other brain neurotransmitters , namely
dopamine and
serotonine . -
pubmed
The QSRR model and DFT /
MP2 studies suggest that retention times of
alkylnaphthyl ketones on
silver - containing stationary phases are determined primarily by the dipole moment , length of the
alkyl substituent and concentration of modifier in the mobile phase . -
pubmed
METHODS : Lipids were extracted from tears and worn contact lenses using 2:1
chloroform :
methanol and the extract washed with aqueous
ammonium acetate , before analysis by electrospray ionization tandem mass spectrometry ( ESI -
MS /
MS ) . -
pubmed
Blockade of
[ 18F ] MK-1312 uptake with
mGluR1 allosteric antagonist
MK-5435 dose - dependently reduced tracer uptake in all regions of gray matter to a similarly low level of tracer uptake . -
pubmed
Taken together , these results are promising for clinical PET studies with
[ 18F ] MK-1312 to determine
mGluR1 occupancy of
MK-5435 . -
pubmed
tRNA : m ( 5 ) C methyltransferase Trm4 generates the modified nucleotide
5 - methylcytidine in archaeal and eukaryotic
tRNA molecules , using
S - adenosyl - l - methionine (
AdoMet ) as
methyl donor . -
pubmed
jannaschii
Trm4 and its complex with
sinefungin at 1.27 A and 2.3 A resolutions , respectively . -
pubmed
Adjacent to the
sinefungin - bound pocket , highly conserved residues form a large , positively charged surface , which seems to be suitable for
tRNA binding . -
pubmed
The short - term success of
imiquimod 5 % cream in randomized controlled trials comparing different treatment regimes and dosing as a treatment for small
superficial basal cell carcinoma (
BCC ) not on the
face or neck is in the range of 82 % for 5 times per week application . -
pubmed
Paracingulin is a 160 - kDa protein localized in the cytoplasmic region of epithelial tight and adherens junctions , where it regulates
RhoA and
Rac1 activities by interacting with
guanine nucleotide exchange factors . -
pubmed
The proportions of NK cells (
CD3 -
CD56 + ) and hematopoetic stem cells (
CD34+lin- ) were increased after
natalizumab treatment . -
pubmed
Drug regimens were categorized into 4 mutually exclusive groups : ( 1 )
tumor necrosis factor (
TNF ) inhibitors with or without other DMARDs ; ( 2 )
methotrexate without
TNF inhibitors or
hydroxychloroquine ; ( 3 )
hydroxychloroquine without
TNF inhibitors or
methotrexate ; or ( 4 ) other nonbiologic DMARDs without
TNF inhibitors ,
methotrexate , or
hydroxychloroquine ( reference exposure ) . -
pubmed
New
diabetes cases and respective incidence rates per 1000 person - years were : other nonbiologic DMARDs ( 55 cases among 3993 treatment episodes ; rate , 50.2 ; 95 % confidence interval [ CI ] , 47.3 - 53.2 ) ;
TNF inhibitors ( 80 cases among 4623 treatment episodes ; rate , 19.7 ; 95 % CI , 19.1 - 20.3 ) ;
methotrexate ( 82 cases among 8195 treatment episodes ; rate , 23.8 ; 95 % CI , 23.0 - 24.6 ) ; and
hydroxychloroquine ( 50 cases among 5682 treatment episodes ; rate , 22.2 ; 95 % CI , 21.3 - 23.1 ) . -
pubmed
The multivariate adjusted hazard ratios for
DM were 0.62 ( 95 % CI , 0.42 - 0.91 ) for
TNF inhibitors , 0.77 ( 95 % CI , 0.53 - 1.13 ) for
methotrexate , and 0.54 ( 95 % CI , 0.36 - 0.80 ) for
hydroxychloroquine compared with other nonbiologic DMARDS . -
pubmed
CONCLUSION : Among patients with
RA or
psoriasis , the adjusted risk of
DM was lower for individuals starting a
TNF inhibitor or
hydroxychloroquine compared with initiation of other nonbiologic DMARDs . -
pubmed
Hippocampi were processed for spectrophotometric measurements of the products of oxidative lipid damage ,
thiobarbituric acid - reactive substances ( TBARS ) levels , as well as the activities of antioxidant enzymes ,
superoxide dismutase (
SOD ) , and
glutathione peroxidase (
GSH - Px ) . -
pubmed
Enoxaparin significantly decreased the hippocampal TBARS and oxidized protein levels ,
COX-2 overexpression and reactive
gliosis , but it did not influence the
SOD and
GSH - Px activities , pro - IL-1beta and active
caspase-3 overexpressions as well as neurodegeneration following
TBI . -
pubmed
METHODS : I have reviewed the literature bases of Google Scholar and the National Library of Medicine using the key words : history , opium , spinal analgesia , spinal
morphine , intrathecal ( IT ) ,
opioid receptors , endogenous opioids , IT delivery of opioids , and IT side - effects / complications . -
pubmed
The depletion of endoplasmic reticulum (
ER )
Ca2 + is sensed by
stromal interaction molecule 1 (
STIM1 ) . -
pubmed
On store depletion ,
STIM1 aggregates and moves to areas where the
ER comes close to the plasma membrane ( PM ; within 25 nm ) to interact with
Orai1 channels and activate
Ca2 + entry . -
pubmed
Greater levels of
GH are induced in T cell - enriched populations compared to B cell - enriched populations after treatment with
CoCl ( 2 ) or
TMA . -
pubmed
Using cultured basal forebrain ( BF ) neurons , this study demonstrates selective internalization of
amyloid beta ( Abeta ) 1 - 42 in conjunction with
p75 ( NTR ) ( labelled with
IgG192 - Cy3 ) by cholinergic cells . -
pubmed
Active under resting conditions , this process was enhanced by high
K (+ ) stimulation and was insensitive to inhibitors of regulated synaptic activity -
tetrodotoxin or
botulinum neurotoxins (
BoNT type / A and / B ) . -
pubmed
Blockade of
sarco - endoplasmic reticulum ( SERCA ) Ca ( 2 + ) ATPase with
thapsigargin and
CPA or chelation of
Ca ( 2 + ) with
EGTA - AM strongly suppressed the endocytosis of
p75 ( NTR ) , implicating the role of
ER released
Ca ( 2 + ) . -
pubmed
The uptake of
IgG192 -
Cy3 was also reduced by
T - type Ca ( 2 + ) channel blocker
mibefradil but not
Cd ( 2 + ) , an indiscriminate blocker of high voltage - activated
Ca ( 2 + ) currents . -
pubmed
A strong co - localization of
IgG192 -
Cy3 with late endosome (
Rab7 ) or lysosome (
Lamp1 ) qualifier proteins suggest these compartments as the primary destination for internalized
IgG192 and
Abeta . -
pubmed
Females then experienced 10 alternating trials of distributed
CLS or no
CLS paired with one side of a non - biased
CPP box under the same hormone - priming regimen . -
pubmed
OBJECTIVES :
Casein phosphopeptide - amorphous
calcium phosphate (
CPP - ACP ) has the potential to induce the biomimetic mineralisation of dentine
collagen fibrils . -
pubmed
This study aimed to demonstrate in vitro the ability of
CPP - ACP to form
apatite crystals on phosphorylated dentine
collagen fibrils . -
pubmed
The slices were etched with
phosphoric acid to expose the
collagen fibres . -
pubmed
Sodium trimetaphosphate was then used to phosphorylate the exposed
collagen fibres . -
pubmed
CPP -
ACP paste was topically applied to the surface of the phosphorylated slices , which were then immersed in a metastable
calcium phosphate remineralising solution and incubated at 37 C for 10 days . -
pubmed
CONCLUSIONS :
CPP - ACP can induce the biomimetic mineralisation of dentine through
apatite formation along and between the phosphorylated dentine
collagen fibres . -
pubmed
Plasma
25 -( OH ) D ,
RBP-4 and
NGAL concentrations were measured with specific assays . -
pubmed
In the entire
BMI range , Spearman correlations revealed strong positive associations between
25 -( OH ) D and
RBP-4 ( r=0.349 , p=0.002 ) and between
25 -( OH ) D and
NGAL ( r=0.338 , p=0.003 ) . -
pubmed
The probes were aggregated nonspecifically to form large clusters , which showed a strong resonance light scattering (
RLS ) peak at 520 nm , under preparation conditions ( pH 7.6
Na2HPO4 -
NaH2PO4 buffer and ultrasonication ) . -
pubmed
Upon addition of
BPA , the probe reacted specifically to form dispersed
BPA - Apt - AuNP conjugates that exhibited strong catalysis of the two particle reactions of
glucose -
Cu ( II ) and
hydrazine hydrochloride -
Cu ( II ) with a strong
RLS peak at 360 nm and 510 nm respectively . -
pubmed
When the
BPA concentration increased , the
RLS intensity at 360 nm and 510 nm increased respectively . -
pubmed
Accordingly , two new and highly - sensitive
RLS methods were established for the detection of
BPA , using the Apt - AuNP catalytic amplification . -
pubmed
AIMS : To examine the associations between antihypertensive treatment with
angiotensin - converting enzyme inhibitors ( ACEis ) or
angiotensin receptor blockers ( ARBs ) , beta - blockers , diuretics , or
calcium - antagonists , and risk of
atrial fibrillation . -
pubmed
The review is presented on the infusion of
sterile water ( hypotonic fluid ) to lower serum
sodium level in those circumstances when enteral supplementation of water is not possible , such as in postoperative patients or when other isotonic fluids ( such as 5 %
dextrose in water infusion ) are less than ideal - for example ,
hyperglycaemic patients on an
insulin infusion . -
pubmed
The significant technical improvement on state - of - the - art is the demonstration of consecutive , spatio - temporally profiling of glycans on a single cell by sequential elution of the previous
lectin probe using their corresponding free
sugar . -
pubmed
METHODS : We present a 4 - year study with 262 unselected consecutive high - risk episodes , prospectively managed with
posaconazole primary prophylaxis and a uniform diagnostic algorithm , including biweekly serum
GM quantification for early detection of invasive
aspergillosis . -
pubmed
The synthesis of the metal - chelator
phytochelatin ( PCs ) and relative levels of transcripts encoding the enzymes
gamma - glutamylcysteine synthetase (
gamma - GCS ) ,
glutathione synthase (
GS ) and
phytochelatin synthase (
PCS ) that participate in the PC biosynthetic pathway were measured , along with the effects on growth , and adsorption and uptake of
Cu . -
pubmed
In Es524 there was an increase in the transcripts of
gamma - GCS ,
GS and
PCS , particularly under high
Cu exposure , whereas in LIA4 transcripts of
gamma - GCS1 increased only slightly ,
gamma - GCS2 and
GS decreased and
PCS did not change . -
pubmed
ABT-639 is a peripherally acting highly selective
T - type Ca ( v ) 3.2 calcium channel blocker that has demonstrated analgesic efficacy in preclinical models and may have the potential to reduce spontaneous fiber activity . -
pubmed
Sudan III fat staining , viability and proliferation assays , electric cell - substrate impedance sensing , real - time PCR for gene expression of
cyclooxygenase-2 and
15 - lipoxygenase and ELISAs for
resolvin D1 (
RvD1 ) ,
IFNgamma ,
TNFalpha and
IL-6 were applied . -
pubmed
IL-6 and
IFNgamma were downregulated in HMGECs treated for 72 h with
DHA and
EPA . -
pubmed
In general ,
TNFalpha ,
IFNgamma and
IL-6 levels were decreased after 72 h compared to 24 h in serum containing medium with or without
DHA or
EPA . -
pubmed
Cyclooxygenase-2 gene expression decreased compared to controls during
DHA stimulation after 72 h. Treatment with
DHA and
ASA revealed a decreased
15 - lipoxygenase gene expression which was reduced after three days of
DHA incubation . -
pubmed
DHA and
EPA supplementation affected HMGECs in vitro and supported anti - inflammatory effects by influencing
cytokine levels , decreasing
COX-2 expression and increasing the production of
RvD1 . -
pubmed
The results showed significant differences between the three
strains which may be explained , at least in part , by variations in the distribution of
carbon source between two glycolytic pathways during the bacterial growth : the
phosphoketolase or heterolactic pathway ( PKP ) and the Embden - Meyerhof pathway ( EMP ) . -
pubmed
Recently , we have provided evidence for therapeutic efficacy of anti -
IGF1R - targeted , surface modified
doxorubicin loaded liposomes . -
pubmed
In the current study ,
post - modification of sterically stabilized liposomal
Dox was thoroughly investigated including the influence of different modification techniques ( PIT , SPIT , SPIT60 ) , lipid composition ( SPC /
Chol , HSPC /
Chol ) , and buffers ( HBS , SH ) . -
pubmed
Prohibitin promotes apoptosis of
promyelocytic leukemia induced by
arsenic sulfide . -
pubmed
Prohibitin (
PHB ) was selected for its diverse role and its increased expression in the cells treated with
As4S4 . -
pubmed
PML -
RARalpha fusion protein was found to be reduced with
PHB - overexpression , and following
As4S4 treatment , a greater reduction of
promyelocytic leukemia - retinoic acid receptor - alpha (
PML - RARalpha ) fusion protein was seen in
PHB - overexpression than that in parental cells . -
pubmed
Niemann - Pick disease type A (
NP - A ) and type B (
NP - B ) are
lysosomal storage diseases (
LSDs ) caused by
sphingomyelin accumulation in lysosomes relying on reduced or absent
acid sphingomyelinase . -
pubmed
AIM OF THE STUDY : Extended spectrum
beta lactamase (
ESBL ) producing bacteria are resistant to most
beta - lactam antibiotics including third - generation
cephalosporins ,
quinolones and
aminoglycosides . -
pubmed
Relationship between plasma and salivary
melatonin and
cortisol investigated by LC -
MS /
MS . -
pubmed
METHODS : Here we describe the validation and application of a high - throughput liquid chromatography in combination with mass spectrometry ( LC -
MS /
MS ) method for the combined analysis of
melatonin and
cortisol in plasma and saliva . -
pubmed
In this study , five
DBS leads were modified using a cost - effective and materials - efficient process for applying an ultra - low impedance
platinum -
iridium alloy coating . -
pubmed
The
platinum -
iridium alloy significantly improved the electrical properties of the
DBS electrodes and was robust to insertion into brain and to 12 weeks of chronic pulsing . -
pubmed
All 18
imipenem - resistant
ceftazidime - susceptible isolates showed decreased mRNA expression of
oprD , and overexpression of
mexB was observed in 13 isolates . -
pubmed
Antibiotics like
bacitracin methylene disalicylate (
BMD ) and
avilamycin (
AVL ) are commonly used antibiotic growth promoters (
AGP ) in poultry feed to control
necrotic enteritis in birds . -
pubmed
As the number of paced beats increased from 1 to 5 ,
SR Ca content ( assessed with
caffeine pulses ) increased , the number of
Ca wave initiation sites increased , integrated
Ca transients and DADs became larger and shorter in duration , and the latency period to the onset of
Ca waves shortened with reduced variance . -
pubmed
In silico analysis using a computer model of ventricular tissue incorporating these experimental measurements revealed that whereas all of these factors promoted larger DADs with higher probability of generating triggered activity , the latency period variance and
SR Ca load had the greatest influences . -
pubmed
Therefore , incorporating quantitative experimental data into tissue level simulations reveals that increased intracellular
Ca promotes DAD - mediated triggered activity in tissue predominantly by increasing both the synchrony ( decreasing latency variance ) of
Ca waves in nearby myocytes and
SR Ca load , whereas the number of
Ca wave initiation sites per myocyte is less important . -
pubmed
Epidermal growth factor signals attenuate phenotypic and functional development of neocortical
GABA neurons . -
pubmed
Phenotypic development of neocortical
GABA neurons is highly plastic and promoted by various
neurotrophic factors such as
neuregulin-1 . -
pubmed
A subpopulation of
GABA neurons expresses not only
neuregulin receptor (
ErbB4 ) but also
epidermal growth factor ( EGF ) receptor (
ErbB1 ) during development , but the neurobiological action of
EGF on this cell population is less understood than that of
neuregulin-1 . -
pubmed
Here , we examined the effects of exogenous
EGF on immature
GABA neurons both in culture and in vivo and also explored physiological consequences in adults . -
pubmed
A
Fe3O4@
Au - basedpseudo - homogeneous electrochemical immunosensor for
AFP measurement using
AFP antibody - GNPs -
HRP as detection probe . -
pubmed
In this study , a
Fe3O4@
Au - based pseudo - homogeneous electrochemical immunosensor was prepared for detection of
alpha fetoprotein (
AFP ) , a well - known
hepatocellular carcinoma biomarker . -
pubmed
The primary antibody (
Ab1 ) was immobilized on
Fe3O4@
Au NPs as the capture probe . -
pubmed
In the presence of
AFP , a sandwich immunocomplex was formed via specific recognition of antigen - antibody in a
Fe3O4@
Au - basedpseudo - homogeneousreaction system . -
pubmed
After the immunocomplex was captured to the surface of magnetic
glassy carbon electrode ( MGCE ) , the labeling
HRP catalyzed the decomposition of
H2O2 , resulting in a substantial current for the quantitative detection of
AFP . -
pubmed
The amperometric ( i - t ) method was employed to record the response signal of the immunosensor based on the catalysis of the immobilized
HRP toward the reduction of
H2O2 with
hydroquinone ( HQ ) as the redox mediator . -
pubmed
On
L1 and
L2 larval stages after 24 h of exposure to extracts , the mortality rates of 100 % , 54.76 % and 96.77 % against 98 % , 51.44 % and 100 % were obtained for
MCM As ,
MCM Nl and
MCM As - Nl respectively at the highest concentration . -
pubmed
Here , we review some of the molecular mechanisms through which
insulin modulates hepatic gluconeogenesis , thus controlling
glucose production by the liver to ultimately maintain normoglycemia . -
pubmed
Codelivery of
doxorubicin and
MDR1 - siRNA by mesoporous
silica nanoparticles -
polymerpolyethylenimine to improve
oral squamous carcinoma treatment . -
pubmed
In this study , we tried to fabricate a novel nanoparticle that could carry both
MDR1 - siRNA to block
MDR1 expression and
doxorubicin (
DOX ) , a chemotherapy drug , into cancer cells in order to directly kill the cells with little or no effect of multidrug resistance . -
pubmed
Results showed that mesoporous
silica nanoparticles ( MSNP ) can be modified by cationic
polymerpolyethylenimine (
PEI ) to obtain positive charges on the surface , which could enable the MSNP to carry
MDR1 - siRNA and
DOX . -
pubmed
Importantly , MSNP -
PEI -
DOX /
MDR1 - siRNA dramatically reduced the
tumor size ( 81.64 % decrease after 28 days posttreatment ) and slowed down
tumor growth rate compared to the control group in vivo ( P<0.05 ) . -
pubmed
In the aggregate , newly synthesized MSNP -
PEI -
DOX /
MDR1 - siRNA improves
cancer chemotherapy effect in terms of treating multidrug - resistant
cancer compared to
DOX only , clearly demonstrating that MSNP -
PEI -
DOX /
MDR1 - siRNA has potential therapeutic application for multidrug - resistant
cancer in the future . -
pubmed
Kyoto Encyclopedia of Gene and Genome analysis showed that the target genes are involved in metabolic pathways ,
mitogen - activated protein kinase signaling pathway ,
calcium signaling pathway , and
nuclear factor - kappaB signaling pathway . -
pubmed
Conclusion :
ESP block is easy to perform and can serve as a promising alternative to
TEA in optimal perioperative
pain management in cardiac surgery . -
pubmed
The resulting
hypercalcemia is a result of
1 - alpha hydroxylase overexpression in
granulomas with increased
1,25 - dihydroxy vitamin D levels . -
pubmed
1,25 - dihydroxy vitamin D levels were markedly elevated with low normal
25 - hydroxy vitamin D levels ,
angiotensin - converting enzyme levels were also high , and chest computed tomography (
CT ) imaging was negative for any
lymphadenopathy ( including perihilar
lymphadenopathy ) . -
pubmed
Consequently , the
CHOP downstream targets such as
phospho -
JNK and
death receptor 5 were also elevated , along with the cleavage of
caspase-8 , - 3 , and the
ER stress - related
caspase-4 . -
pubmed
We have now investigated whether depletion of the recently identified third
galanin receptor ,
GAL3 - R , and that of
GAL2 - R , alters the threshold to the systemically applied
gamma - aminobutyric acid (
GABA ) antagonist
pentylenetetrazole (
PTZ ) or to intrahippocampally administered
kainic acid (
KA ) . -
pubmed
Amplicon sequencing of the hypervariable V3 - V4 regions of the
16S rRNA gene revealed that microbial communities remained stable across
CO2 seep sites ( pH = 7.44 - 7.85 ) and adjacent control sites ( ambient pH = 8.0 - 8.1 ) . -
pubmed
Parameters of
glucose and lipid metabolisms were measured for the pups on
post - natal day 21 , 26 and 60 . -
pubmed
At weaning ,
insulin secretion upon
glucose stimulation was unchanged , but the removal of circulating
glucose was slower for pups born from the lowest impregnated dams ( Cd1 ) . -
pubmed
Furthermore , pups from dams accumulating the highest levels of
Cd (
Cd2 ) exhibited a compensatory increased
insulin pancreatic secretion , together with increased circulating non - esterified
fatty acids , indicating the establishment of
insulin resistance , 2 months after birth . -
pubmed
In this paper , a facile one -
pot freeze - drying self - assembly approach combined with in - situ thermal decomposition - reduction method is proposed to construct 3D porous aerogels with
MoS2 active materials anchored on
graphene backbone , making a highly interconnected network with desirable structural stability . -
pubmed
We compared
DON with a state - of - art disease phenotype network , disease manifestation network (
DMN ) , that we have recently constructed , and demonstrated that
DON contains complementary knowledge for
disease genetics understanding . -
pubmed
OBJECTIVES : Primary objectivesTo determine the effectiveness and safety of
ibuprofen compared to placebo / no intervention , or other
cyclo - oxygenase inhibitor drugs in the prevention of
PDA in
preterm infants . -
pubmed
SELECTION CRITERIA : Randomised and quasi - randomised controlled trials comparing
ibuprofen with placebo / no intervention or other
cyclo - oxygenase inhibitor drugs to prevent
PDA in preterm or low
birth weight infants . -
pubmed
Ibuprofen (
IV or oral ) probably decreases the risk of
PDA on day 3 or 4 ( typical RR 0.39 , 95 % CI 0.31 to 0.48 ; typical
RD -0.26 , 95 % CI -0.31 to -0.21 ; NNTB 4 , 95 % CI 3 to 5 ; 9 trials ; N = 1029 ) ( moderate - quality evidence ) . -
pubmed
In addition ,
ibuprofen probably decreases the need for rescue treatment with
cyclo - oxygenase inhibitors ( typical RR 0.17 , 95 % CI 0.11 to 0.26 ; typical
RD -0.27 , 95 % CI -0.32 to -0.22 ; NNTB 4 ; 95 % CI 3 to 5),and the need for surgical ductal ligation ( typical RR 0.46 , 95 % CI 0.22 to 0.96 ; typical
RD -0.03 , 95 % CI -0.05 to -0.00 ; NNTB 33 , 95 % CI 20 to infinity ; 7 trials ; N = 925 ) ( moderate - quality evidence ) . -
pubmed
AUTHORS ' CONCLUSIONS : This review shows that prophylactic use of
ibuprofen , compared to placebo or no intervention , probably decreases the incidence of
patent ductus arteriosus , the need for rescue treatment with
cyclo - oxygenase inhibitors , and for surgical ductal closure . -
pubmed
Adverse effects associated with
ibuprofen (
IV or oral ) included increased risks for
oliguria , increase in serum
creatinine levels , and increased risk of
gastrointestinal haemorrhage . -
pubmed
Transient
tyrosine and
tryptophan radicals play key roles in the electron transfer (
ET ) reactions of
photosystem ( PS ) II ,
ribonucleotide reductase (
RNR ) ,
photolyase and many other proteins . -
pubmed
Cytochrome c peroxidase (
CcP ) employs a
Trp191 + radical to oxidize reduced
cytochrome c ( Cc ) . -
pubmed
Here we probe the redox properties of
CcP Y191 by non - natural
amino acid substitution , altering the
ET driving force , and manipulating the protic environment of Y191 . -
pubmed
Taken together , studies of inhibition of hemagglutinating activity , fluorescence spectroscopy and molecular docking revealed that
gentamicin can interact with
DVL via the
carbohydrate recognition domain (
CRD ) , suggesting that the results obtained in this study may be directly related to the interaction of
DVL -
gentamicin and with the ability of the
lectin to interact with glycans present in the cells of the peritoneum . -
pubmed
Trends in HbA1c and
LDL Cholesterol in Patients With
Type 2 Diabetes Receiving First - Time Treatment in Northern Denmark , 2000 - 2017 : Population - Based Sequential Cross - Sectional Analysis . -
pubmed
We first use a
B12 - responsive transcription factor to enable
B12 - dependent output in a cell - free reaction , but the resulting sensor responds to
B12 far above clinically relevant concentrations . -
pubmed
Blood samples were probed for LPS - mediated
cytokine production by incubating them with LPS inhibitor
polymyxin B (
PMB ) for 24 h at 37 C besides the Limulus amebocyte lysate ( LAL ) assay . -
pubmed
PMB suppressed
IL-1beta ( P = 0.035 ) and
IL-6 ( P = 0.0487 ) production in the 3 h PPB of the PS after 24 h incubation at 37 C compared to the vehicle control , suggesting the presence of LPS . -
pubmed
Sulfated
vizantin disrupted the localization balance between cell - associated
glucosyltransferase and cell - free
glucosyltransferase , resulting in inhibited biofilm maturation . -
pubmed
The
KIRs and
HLA ligands were identified in 235 healthy controls (
HC ) and 253
dengue patients ( DEN ) using
polymerase chain reaction with sequence specific primer ( PCR - SSP ) ; moreover ,
DEN was classified to 100
dengue fever (
DF ) and 153
dengue haemorrhagic fever (
DHF ) . -
pubmed
Other quality indexes of soy sauce , including
RS , TA , and
AN , were not greatly affected . -
pubmed
The results illustrate the accuracy of the Figaro
TGS 2600 sensor when
methane is quantified from raw signals using the techniques described . -
pubmed
A possibility that the anticataleptic action of
chlorcyclizine ,
cyproheptadine ,
diphenhydramine ,
mepyrymine and
danitracen depends on the blockade of the
histamine receptors in the brain is discussed . -
pubmed
The effect of
delagil (
chloroquine ) and of
hydrocortisone taken in different concentration on the lymphocytes blast - transformation reaction in donors , stimulated with
streptococcal nucleoprotein , was enquired into . -
pubmed
The excretion profile of the combined hydrolysis products was compared with the excretion profile of a different group of metabolites of
sulfur mustard derived from the
glutathione / beta - lyase pathway . -
pubmed
The excretion of
beta - lyase products showed a much sharper decline than was observed for the hydrolysis products of
sulfur mustard . -
pubmed
Mutant
Era - V17 purified from the
strain was cold - sensitive for
GTP - hydrolytic activity , suggesting that the
GTPase activity of
Era is required for cell growth since the P17V mutation resulted in both cold - sensitive growth of cells and cold - labile
GTPase activity of the purified protein . -
pubmed
Interstitial cells were prepared by
collagenase digestion and used to measure
125I - labelled
human chorionic gonadotrophin (
hCG ) binding capacity and
androgen production in the presence or absence of
hCG or
dibutyryl cyclic AMP (
dbcAMP ) . -
pubmed
At day 2 after
EDS treatment ,
125I - labelled
hCG binding capacity was reduced to 10 % of control values , while the production of
testosterone and
5 alpha - androstane-3 alpha , 17 beta - diol (
adiol ) were non - detectable . -
pubmed
Between days 24 and 40
post - treatment , Leydig cell regeneration occurred , as indicated by a rise in
125I - labelled
hCG binding capacity , increased
androgen production and the presence of histologically identifiable Leydig cells . -
pubmed
Western blots with anti -
tubulin antibody showed that the
albendazole resistant
strain had an altered
tubulin . -
pubmed
It is postulated that
bldA specifies a
tRNA that would recognize the codon UUA ( for
leucine ) . -
pubmed
Previous studies have demonstrated that near - complete immunosuppression is obtained with FK at 10(-8 ) M and
CsA at 10(-6 ) M. When cultured up to 72 h throughout the concentration range , neither FK nor
CsA altered HFP cellular architecture , and indirect immunoperoxidase staining for
insulin demonstrated no change in quantity or distribution of
insulin - producing beta - cells compared with control tissue . -
pubmed
Drug - cultured HFP also retained the ability to release
insulin in response to a
glucose /
theophylline challenge . -
pubmed
In
calcium containing buffer ,
endothelin-1 induced a concentration - dependent
45Ca2 + efflux response over the range of 10 pM to 100 nM with an EC50 of approximately 60 pM. -
pubmed
Maximum
endothelin - stimulated
45Ca2 + efflux was not affected by the absence of extracellular
calcium or the presence of 1 microM
verapamil . -
pubmed
Endothelin-1 did not induce transplasmalemmal
45Ca2 + uptake at times up to 30 min . -
pubmed
Staining was minimal or absent for
protein C ,
protein S ,
plasminogen activator inhibitors 1 - 3 ,
factor VII ,
factor X , and
fibrin ( the antigenic site on the
amino - terminal portion of
B beta chain that is exposed following
thrombin cleavage of
fibrinopeptide B was not detected ) . -
pubmed
The precursor contains three functional domains , the
amino - terminal leader which is assumed to initiate the inner membrane transport of the precursor , the
protease , and a
carboxyl - terminal ' helper ' domain apparently required for extracellular secretion ( excretion ) . -
pubmed
Unaccounted for by an increase in viable ovarian cell numbers and independent of the
hCG dose ( 0.1 - 10 ng / ml ) used , the Sm - C /
IGF - I effect proved time and dose dependent , with a projected minimal effective dose of 3 ng / ml and a minimal time requirement of 72 h.
[ 125I ] Iodo - Sm - C /
IGF - I binding to untreated highly enriched thecal - interstitial cells proved saturable , with a single class ( Hill coefficient = 0.98 + /- 0.01 ) of high affinity ( Kd = 3.0 nM ) , low capacity ( maximum binding = 10,840 + /- 2,108 sites / cell ) binding sites . -
pubmed
Taken together , these findings suggest that 1 ) the thecal - interstitial cell , like the granulosa cell , may be a site of Sm - C /
IGF - I reception and action , and 2 ) the ability of high dose
insulin to stimulate ovarian
androgen biosynthesis may be due to its capacity to act as a Sm - C /
IGF - I surrogate , its high dose requirements reflecting cross - interaction with the type I receptor. -
pubmed
PAMP-1 has generally been regarded as an '
oestrogen - inducible ' protein regulated by the
oestrogen /
androgen balance . -
pubmed
Lipolysis was measured by
glycerol release after either incubation or perifusion with the following concentrations :
epinephrine (
EPI ) , 0.01 - 0.1 microM ;
theophylline , 0.01 - 1.0 mg / ml ;
adenosine deaminase (
ADA ) , and
bovine GH (
bGH ) , 0.01 - 1.0 microgram / ml . -
pubmed
In a dose - response study comparing
glycerol release from control and
STZ - DM IFC , IFC were preincubated with 1.0 microgram / ml
bGH and then incubated with varying concentrations of
EPI or
bGH . -
pubmed
In
STZ - DM , we noted increased lipolytic sensitivity to low concentrations of
EPI or
bGH . -
pubmed
Furthermore , in perifusion ,
STZ - DM IFC did not require obligatory preincubation with
bGH for optimal
glycerol release . -
pubmed
In both systems an enhanced lipolytic response to
theophylline was seen in the presence of
bGH in control and
STZ - DM . -
pubmed
Preincubation with
GH increases the lipolytic response of IFC from
STZ - DM to all lipolytic agents compared to control responses . -
pubmed
One of these , the
cytochrome - based electron transport system , produced anaerobic conditions by the reduction of
oxygen dissolved in the medium . -
pubmed
The reactions of the
sulfhydryl groups on the
elongation factors Tu and
Ts . -
pubmed
The observed changes in enzymic activities are consistent with the possibility that
RFP induces a special form of
cytochrome P-450 , responsible for the metabolism of the antibiotic ( demethylation and reduction of
rifampicin quinone ) . -
pubmed
Cytochrome P-450 concentration was not greatly affected , with only a 40 % increase by
clofibrate ( dose 200 mg / kg / day ) and by
F1379 at the lower dose . -
pubmed
It is of interest that all the hypolipidaemic agents tested enhanced the activity of
epoxide hydrolase with
4 , 5 benzo ( a ) pyrene oxide as the substrate . -
pubmed
We also measured
UDP - glucuronosyltransferase activities using two substrates belonging to group I (
4 - nitrophenol ) and group
II (
4 - hydroxybiphenyl ) . -
pubmed
This study showed that hypolipidaemic drugs which are chemically related to
clofibrate could greatly modify the activity of
drug metabolizing enzymes and therefore alter the transformation of
drugs administered concomitantly. -
pubmed
Membrane modification ( administration of
cholesterol ,
trypsin ) influences the PC exchange ; changes of microviscosity brings about the exchange , but the interrelation is of very complicated character . -
pubmed
D. Kallikrein -
kinin system and
prostaglandins system ] . -
pubmed
Commercially available
concanavalin A binds
Ca2 + with high apparent affinity . -
pubmed
The
concanavalin A stimulated uptake of
45Ca2 + occurred within minutes , and required concentrations of concanavalin A which promoted
[ 3H ] thymidine uptake into these cells . -
pubmed
Although the concanavalin A - promoted
Ca2 + uptake occurs at
lectin concentrations that cause lymphocyte proliferation as measured by
3H - thymidine incorporation , the role of
Ca2 + in this event remains unclear . -
pubmed
CONCLUSIONS : The phase
II dose of
doxorubicin in this combination therapy has been established at 165 mg / m2 , with dose - limiting
toxicity defined as
mucositis . -
pubmed
The two alleles were found to differ by a single TA to
GC transversion , altering one
amino acid in the deduced
amino acid sequence . -
pubmed
We tested the hypothesis that muscle cell types at
rest have intrinsically different contents of
PCr ,
ATP and Pi . -
pubmed
The
retinol level below 30
MK2 / dl ( lower reference level ) was revealed in 6 % of the adults , 19 % of the children and 6 % of the pregnant women . -
pubmed
The level of
carotene below 80
MK2 / dl took place in 39 % of the adults , 28 % of the children and 18 % of the pregnant women . -
pubmed
Effects of
HMG - CoA reductase inhibition on erythrocyte membrane
cholesterol and
acyl chain composition . -
pubmed
These results suggest that the chemical potential of
cholesterol in serum is not markedly altered by
HMG - CoA reductase inhibition . -
pubmed
Variation in sulfation pattern on
aggrecan chondroitin
sulfate side chains is also observed in the
hypertrophic region with an increasing proportion of unsulfated residues present , which may play a role in the initiation of mineralization . -
pubmed
The existence of
cryptic genes , responsible for growth on alternative substrates , was previously deduced from the analysis of other mutants obtained as a response to the restrictive condition on media containing non - assimilating
carbon sources . -
pubmed
Regulation of the
adenohypophyseal thyrotropin - releasing hormone - degrading ectoenzyme by
estradiol . -
pubmed
We describe here gender - related differences and the effect of
estradiol (
E2 ) on the expression of this enzyme in the anterior pituitary . -
pubmed
Because the expression of the
adenohypophyseal TRH - degrading ectoenzyme is tightly regulated by both T3 and
E2 with adequate dynamics , we conclude that this peptidase serves integrative functions for the control of
TRH - stimulated hormone secretion . -
pubmed
In contrast to
PEC-60 and
somatostatin , it has no established inhibitory effect on
insulin secretion . -
pubmed
Like gastrointestinal trefoil peptides ,
dopuin has three
disulphide bridges ,
Ala - Pro segments , and many charged residues , but they are differently distributed and
dopuin belongs to a separate , apparently novel family . -
pubmed
However , the tension - time integrals indicated an equivalent capacity of sequestrable
Ca2 + in
SR from both tissues . -
pubmed
In atrial , as in ventricular myocardium , the
SR Ca2 + uptake was more efficiently supported by
ATP produced by the SR - bound MM form of
creatine kinase (
CK ; MM -
CK ) than by externally added
ATP , suggesting a tight functional coupling between the
SR Ca2 + adenosinetriphosphatase (
ATPase ) and
MM - CK . -
pubmed
CaMKIIPase showed apparent molecular weights of 54,000 and 65,000 , on
SDS - polyacrylamide gel electrophoresis and gel - filtration analysis , respectively . -
pubmed
The stimulated
Ca ( 2 +)-independent activity of autophosphorylated
CaMKII was reversed by the action of
CaMKIIPase . -
pubmed
Insulin secretion in response to an oral
glucose tolerance test ( OGTT ) and a
GH stimulation test by
L - dopa ,
growth hormone - binding protein (
GHBP ) and
IGF - I were measured . -
pubmed
The
insulin and free
fatty acid ( FFA ) areas under curves ( AUC ) during the OGTT , and the
IGF - I level , were also positively correlated with
GHBP levels ( r = 0.474 , P<0.005 ; r = 0.572 , P<0.005 ; r = 0.453 . -
pubmed
GH - AUC to the
L - dopa stimulation test was negatively correlated with
GHBP levels ( r = -0.432 . -
pubmed
This activation is dependent on the presence of high molecular weight
kininogen and an optimal
zinc (
Zn2 + ) concentration . -
pubmed
Further
kallikrein formed on endothelial cell membranes is capable of cleaving its receptor and native substrate , high molecular weight
kininogen , liberating
bradykinin and terminating activation . -
pubmed
h(-1)provided an anti -
thrombotic effect which is at least comparable to an activated partial
thromboplastin time adjusted
heparin infusion . -
pubmed
The level of
thrombin inhibition by
efegatran , as measured by activated partial
thromboplastin time , appeared to be more stable than with
heparin . -
pubmed
Thus , like other
thrombin inhibitors ,
efegatran sulphate is easier to administer than
heparin . -
pubmed
In the present study , the effects of
macrophage CSF (
M - CSF ) ,
G - CSF ,
GM - CSF and
interleukin-3 (
IL-3 ) ( 10 ng / ml ) on the production of
nitric oxide and
prostaglandin E2 (
PGE2 ) by cartilage explants were examined over 24 and 48 h. The effects of these
CSFs were also measured in combination with
IL-1 beta ( 10 ng / ml ) . -
pubmed
M - CSF ,
GM - CSF and
IL-3 had no effect on
nitrite production . -
pubmed
However , both
IL-1 beta and
G - CSF caused a significant increase ( p & lt ; 0.05 ) in
nitrite levels at 48 h.
NG - L - arginine - methyl - ester was used to inhibit
nitrite production induced by
G - CSF and this implicated
nitric oxide synthase activity . -
pubmed
When
G - CSF and
IL-1 beta were used in a combined treatment ,
nitrite levels were significantly increased ( p & lt ; 0.05 ) at both 24 and 48 h. Both
IL-3 alone and in combination with
IL-1 beta caused elevated
PGE2 production in this model . -
pubmed
PGE2 levels were also significantly increased by stimulation with
GM - CSF and
IL-3 combined with
IL-1 beta . -
pubmed
These findings demonstrate that
GM - CSF ,
G - CSF and
IL-3 may induce changes in the production of inflammatory mediators such
nitric oxide and
PGE2 in cartilage chondrocytes . -
pubmed
Serotonin 5 - HT ( 2 ) receptors activate local
GABA inhibitory inputs to serotonergic neurons of the dorsal raphe nucleus . -
pubmed
The purpose of the present study was to characterize the synaptic currents induced by bath - applied
serotonin (
5 - HT ) in 5 - HT cells of the dorsal raphe nucleus ( DRN ) and to determine which
5 - HT receptor subtypes mediate these effects . -
pubmed
These effects were blocked by the
GABA ( A ) receptor antagonist ,
bicuculline ( 10 microM ) and by the fast
sodium channel blocker ,
TTX , suggesting that
5 - HT had increased impulse flow in local GABAergic neurons . -
pubmed
A near maximal concentration of the selective
5 - HT ( 2A ) antagonist ,
MDL100,907 ( 30 nM ) , produced a large reduction ( approximately 70 % ) in the increase in IPSC frequency induced by 100 microM
5 - HT ;
SB242,084 ( 30 nM ) , a selective
5 - HT ( 2C ) antagonist , was less effective ( approximately 24 % reduction ) . -
pubmed
Combined drug application suppressed the increase in
5 - HT - induced IPSC frequency almost completely , suggesting involvement of both
5 - HT ( 2A ) and
5 - HT ( 2C ) receptors . -
pubmed
Unexpectedly , the
phenethylamine hallucinogen ,
DOI , a partial agonist at
5 - HT ( 2A / 2C ) receptors , caused a greater increase ( +334 % ) in IPSC frequency than did 5 - HT 100 microM ( +80 % ) . -
pubmed
This result may be explained by an opposing
5 - HT ( 1A ) inhibitory effect since the selective
5 - HT ( 1A ) antagonist ,
WAY-100635 , enhanced the 5 - HT - induced increase in IPSCs . -
pubmed
These results indicate that within the DRN -
PAG area there may be a negative feedback loop in which
5 - HT induces an increase in IPSC frequency in 5 - HT cells by exciting GABAergic interneurons in the DRN via
5 - HT ( 2A ) and , to a lesser extent ,
5 - HT ( 2C ) receptors . -
pubmed
The Endologix device , currently being acquired by
CR Bard , Incorporated , is a new , one - piece self - expanding
polytetrafluoroethylene prosthesis that offers an alternative to current modular and fabric devices . -
pubmed
He was found to have a subnormal
cortisol level of 2.3 microg / dL at baseline , which increased to only 5.6 microg / dL 60 minutes after a 250 - microg
corticotropin intravenous stimulation test . -
pubmed
Binding of the
C4BP - protein S complex to apoptotic cells was
calcium - dependent and could be blocked with
Abs directed against the phospholipid - binding domain in
protein S. -
pubmed
Annexin V , which binds to exposed
phosphatidylserine on the apoptotic cell surface , could inhibit the binding of
protein S. -
pubmed
Both species showed increased
ferric reductase activity upon
Fe starvation , but they differed in the maximum rates . -
pubmed
Changes in the distribution of
saturated and unsaturated fatty acids (
FA ) at 30 and 37 degrees C were considered as one of possible causes of the
TDS phenomenon . -
pubmed
At identical temperatures , no statistically significant differences in the distribution of major
FA (
iso-15:0 ,
anteiso-15:0 , 16:0 and 16:1 ) were registered between individual groups . -
pubmed
BACKGROUND : The cardiovascular effects of
angiotensin II are mediated by the
angiotensin II type 1 receptor (
AGT1R ) ; one polymorphism of the
AGT1R gene , A1166 - ->C , has been associated with
hypertension . -
pubmed
The hemodynamic response to
angiotensin II is blunted in women compared to men , but interactions between gender , blood pressure , and
AGT1R gene polymorphisms are unclear . -
pubmed
In addition , the renal hemodynamic response to
angiotensin II infusion is a function of both gender and the
AGT1R genotype . -
pubmed
Interaction of
chlorisondamine with the
neuronal nicotinic acetylcholine receptor . -
pubmed
An epitope was found on the alpha2 - nicotinic isoform of the
neuronal nicotinic acetylcholine receptor that would likely form salt bridges with
quaternary ammonium compounds and a cation - pi interaction with the pi - cloud of an aromatic ring . -
pubmed
The third complementarity - determining region (
CDR ) of the
TCR alpha and beta chains forms loops that engage
amino acid residues of peptides complexed with
MHC . -
pubmed
The joining of these gene segments is imprecise , leading to significant variability in the
TCRbeta chain
CDR3 loop length and
amino acid composition . -
pubmed
Moreover , biochemical analyses (
G6PD ,
CAT ,
SOD ,
GSH and
MDA ) were determined in supernatant of kidney tissue . -
pubmed
GLYCINE - SERUM INTERCONVERSION AND
COLLAGEN BIOSYNTHESIS IN VIVO . -
pubmed
POSSIBLE DIFFERENCE IN THE
AMINO ACID SUPPLYING SYSTEM BETWEEN SKIN
COLLAGEN AND
SERUM ALBUMIN IN GUINEA PIGS . -
pubmed
AIM : The aim of the present experiment was to study the effect of different
chlorhexidine regimens on the number of bacteria in saliva , and on de novo
plaque formation . -
pubmed
In
breast cancer patients ,
tamoxifen or
aromatase inhibitors can be used for ovarian stimulation prior to oocyte or embryo cryopreservation . -
pubmed
NE , NPY ,
ATP , and a
P2 - receptor analogue were added before
S2 . -
pubmed
NPY ( 0.05 - 1.0 micromol / l ) concentration dependently inhibited
acetylcholine release . -
pubmed
This effect was prevented by the
NPY - Y ( 2 )- receptor antagonist
BIIE 0246 ( 0.1 micromol / l ) but not by the
NPY - Y ( 1 )- receptor antagonist
BIBP 3226 ( 10 micromol / l ) . -
pubmed
Unprecedented
olefin - dependent histidine - kinase inhibitory of
zerumbone ring - opening material . -
pubmed
After withdrawing
tetracycline , the
C - JNK fragment expression was induced and cell growth was dramatically inhibited 24 h later . -
pubmed
Pre - incubation of cells with blocking agents of epithelial
Na (+ ) channel (
ENaC ) for 15 min ( normal [
NaCl ] throughout ) caused
Cu accumulation rates to increase by 40 - fold ( 100 micromol l(-1 )
phenamil ) , 21 - fold ( 10 micromol l(-1 ) CDPC ) or 12 - fold ( 2 mmol l(-1 )
amiloride ) when
Cu ( o ) was 800 micromol l(-1 ) compared to those in drug - free controls . -
pubmed
We conclude that
Cu accumulation by intestinal cells is mainly
Na(+)-insensitive and more characteristic of a pH- and
K (+)-sensitive
Ctr1 - like pathway than
Cu uptake through
ENaCs . -
pubmed
Membrane - associated
NADPH oxidase complexes catalyse the production of the
superoxide anion radical from
oxygen and
NADPH . -
pubmed
Simvastatin significantly improved
CLP - induced mortality and
AKI . -
pubmed
Simvastatin attenuated
CLP - induced
tubular damage and reversed
CLP - induced reduction of intrarenal microvascular perfusion and renal tubular
hypoxia at 24 h.
Simvastatin also restored towards normal CLP - induced renal vascular protein leak and serum
TNF - alpha . -
pubmed
Neither delayed
simvastatin therapy nor
TNF - alpha neutralizing antibody improved
CLP - induced
AKI . -
pubmed
We present synthetic examples that validate our approach and show a comparison between existing segmentation techniques of paranasal
sinus CT data . -
pubmed
RESULTS : The levels of
superoxide dismutase (
SOD ) and
nitric oxide (
NO ) increased more significantly in the Shenfu group than in the control group ( P<0.05 ) . -
pubmed
The levels of serum liver enzymes ,
hyaluronic acid ( HA ) ,
malondialdehyde (
MDA ) ,
tumor necrosis factor - alpha (
TNF - alpha ) ,
interleukin-1 (
IL-1 ) ,
endothelin - 1 (
ET-1 ) and liver cell apoptosis index were lower in the Shenfu group than in the control group ( P<0.05 ) . -
pubmed
Bioisosteric modification of
salvinorin A , a potent and selective
kappa - opioid receptor agonist . -
pubmed
Salvinorin A (
( 2S,4aR,6aR,7R,9S,10aS , 10bR )- 2H - naphtho [ 2,1 - c ] pyran-7 - carboxylic acid ,
9 -( acetyloxy )- 2 -( 3 - furanyl ) dodecahydro-6a,10b - dimethyl-4,10 - dioxo methyl ester , 1 ,
CAS 83729 - 01 - 5 ) has been shown to bind with high affinity and selectivity to the
kappa - opioid receptor (
KOR ) as an agonist . -
pubmed
It is now apparent that these and likely other additional
molecules are required along with
ORs to orchestrate the complex pattern of convergence and divergence that is unique to the olfactory system . -
pubmed
The expression of the
haemolysin also increased under
iron -
starvation conditions . -
pubmed
Identification of the target compound was performed by
( 1 ) H NMR ,
( 13 ) C NMR , two - dimensional NMR , electrospray ionization
MS , IR and UV . -
pubmed
Association of
ABCB1 /
MDR1 and
OPRM1 gene polymorphisms with
morphine pain relief . -
pubmed
We focused on two such genes :
ABCB1 /
MDR1 , a major determinant of
morphine bioavailability , and
OPRM1 , which encodes for the
mu - opioid receptor , the primary site of action for
morphine . -
pubmed
The isolation procedure described here is relatively brief and relies on the powerful proteolytic activity of
proteinase K combined with the denaturing ability of the ionic detergent
SDS . -
pubmed
The
[ H - C ] and
[ C - P ] bond lengths of
HCP are set to their values at the linear equilibrium ground vibrational level of the molecule . -
pubmed
, T<or=70 K. These quantities are significantly magnified in comparison with their counterparts for the
HCP -
He collisions . -
pubmed
Interactions among
type I and type II interferon ,
tumor necrosis factor , and
beta - estradiol in the regulation of immune response - related gene expressions in
systemic lupus erythematosus . -
pubmed
Since interactions among
TNF ,
IFNgamma ,
beta - estradiol (
E2 ) , and
IFNalpha may regulate the expression of interferon - inducible ( IFI ) genes , stimulating and co - stimulating experiments were carried out on peripheral blood mononuclear cells followed by analysis using quantitative RT - PCR . -
pubmed
A subset of 35 children underwent a meal test with blood draws to measure
insulin and
glucose . -
pubmed
CYP2D6 and
CYP3A5 genotypes were determined , and the plasma levels of
RIS and
9 - OH - RIS were measured using high - performance liquid chromatography . -
pubmed
The dose - normalized plasma concentrations of
RIS ,
9 - OH - RIS , and the active moiety were compared according to the
CYP2D6 and
CYP3A5 genotypes . -
pubmed
The plasma levels of
RIS ( 2.03 ng / mL per milligram for EMs vs 5.57 ng / mL per milligram for PMs , P & lt ; 0.001 ) and
9 - OH - RIS ( 5.06 ng / mL per milligram for EMs vs 0.22 ng / mL per milligram for PMs , P & lt ; 0.001 ) were significantly different among
CYP2D6 genotype groups , but the
CYP2D6 EMs ( 7.09 ng / mL per milligram ) and PMs ( 5.79 ng / mL per milligram ) did not show no difference in the levels of the active moiety ( P = 0.470 ) . -
pubmed
CYP3A5 nonexpressors exhibited higher plasma concentrations of both
RIS and
9 - OH - RIS than its expressors . -
pubmed
In the case of
9 - OH - RIS ,
CYP3A5 nonexpressors exhibited significantly higher concentrations than
CYP3A5 expressors ( 5.42 vs 3.51 ng / mL per milligram , P = 0.022 ) . -
pubmed
In conclusion , both
CYP2D6 and
CYP3A5 genotypes affected plasma levels of
RIS and
9 - OH - RIS , whereas the active moiety levels were influenced only by the
CYP3A5 genotype but not by the
CYP2D6 genotype . -
pubmed
The VOC - responsive proteins contain
ATP synthase CF1 ,
ribulose-1,5 - bisphosphate carboxylase / oxygenase ,
photosystem II light harvesting complex , and
enolase , which are components of photosynthesis and
carbohydrate metabolism . -
pubmed
Based on
RTS analysis for various temperatures , gate bias and optical power we determined the characteristics of these single photogenerated traps : the energy position within the
silicon bandgap , capture cross section and the position within the
Si /
SiO(x = 1.5 ) interfaces . -
pubmed
A novel method has been developed to detect two organic
arsenic animal feed additives including
roxarsone and
p - arsanilic acid , as well as other
arsenic species such as
arsenite ,
dimethylarsinic acid ,
monomethylarsonic acid ,
arsenate , and
4 - hydroxyphenylarsonic acid , by using high - performance liquid chromatography coupled to an inductively coupled plasma mass spectrometer ( HPLC - ICP -
MS ) . -
pubmed
Liquid chromatography coupled to tandem mass spectrometry ( LC -
MS /
MS ) was used as an assistant tool to screen
arsenobetain (
AsB ) in the feed samples by monitoring the reaction at m / z 179 - ->120 . -
pubmed
The ion - pair reversed phase HPLC - ICP -
MS method was then successfully applied to the speciation of
arsenic in feedstuff and formula feed samples . -
pubmed
Physiological parameters measured were
pulse oxygen saturation ( SpO(2 ) ) , end - tidal
CO ( 2 ) ( EtCO ( 2 ) ) , heart rate (
HR ) , and respiratory rate ( RR ) . -
pubmed
Flavin mononucleotide - based fluorescent reporter proteins outperform
green fluorescent protein - like proteins as quantitative in vivo real - time reporters . -
pubmed
We identify more than 100 metabolites , including
orsellinic acid derivatives , accumulating preferentially in the
csnE mutant . -
pubmed
Bronchoscopic Nd : YAG laser irradiation , argon plasma coagulation (
APC ) and drugs administration such as
steroids and
colchicines were reported to be effective in some patients . -
pubmed
The
multikinase inhibitor
sorafenib (
Nexavar , Bayer ) , used in the treatment of
renal and hepatocellular carcinoma , suppresses
tumor angiogenesis and promotes autophagy in
tumor cells . -
pubmed
Tumor cell types that displayed high levels of cell killing after combination treatment showed elevated levels of
AKT ,
p70 S6 K , and/or phosphorylated
mTOR , in addition to
class III receptor tyrosine kinases such as
platelet - derived growth factor receptor beta and
VEGF receptors , known in vivo targets of
sorafenib . -
pubmed
Importantly , slices responded to the application of
E-4031 ,
chromanol and
4 - aminopyridine , three
potassium channel blockers known to affect action potential duration , with an increase in FPD . -
pubmed
In vitro selection of variants resistant to
beta - lactams plus
beta - lactamase inhibitors in
CTX - M beta - lactamases : predicting the in vivo scenario ? -
pubmed
The therapeutic options for
CTX - M - carrying isolates are scarce , forcing the reexamination of the therapeutic possibilities of
beta - lactams plus
beta - lactamase inhibitors ( BBLIs ) . -
pubmed
Fourteen
CTX - M beta - lactamases belonging to the three most representative clusters (
CTX - M-1 , -2 , and -9 ) and the two main phenotypes (
cefotaxime resistance and
cefotaxime -
ceftazidime resistance ) were studied . -
pubmed
The most frequently recovered mutation was S130 G , which conferred the highest resistance levels to BBLIs ( reaching 12 mug / ml for
amoxicillin - clavulanate and 96 mug / ml for
piperacillin - tazobactam when acquired by
CTX - M-1 cluster enzymes ) . -
pubmed
The
glmS ribozyme tunes the catalytically critical pK(a ) of its coenzyme
glucosamine-6 - phosphate . -
pubmed
Here we used Raman crystallography directly to determine the pK(a ) 's of
GlcN6P bound to the
glmS ribozyme . -
pubmed
Moreover , the anionic
glmS ribozyme in effect stabilizes the neutral
amine of
GlcN6P by lowering its pK(a ) . -
pubmed
Lipid peroxidation , glycogen content of liver and skeletal muscles ,
insulin ,
superoxide dismutase (
SOD ) ,
catalase (
CAT ) ,
glutathione (
GSH ) ,
oxidized glutathione (
GSSG ) levels were determined . -
pubmed
Chloroform extract from AI shows increased levels of
SOD ,
GSH ,
GSSG and
CAT , hepatic glycogen content ,
glucose-6 - phosphatase and
insulin plasma levels , which also decreased the
glucokinase (
GK ) , lipid peroxidation and
insulin resistance . -
pubmed
Increased serum
PCSK9 concentrations are associated with
periodontal infection but do not correlate with
LDL cholesterol concentration . -
pubmed
Recent studies demonstrated that
proprotein convertase subtilisin / kexin type 9 (
PCSK9 ) plays a critical role in circulating
LDL cholesterol concentrations . -
pubmed
Rapid Disruption of Cellular Integrity of
Zinc - treated Astroglia Is Regulated by
p38 MAPK and
Ca - dependent Mechanisms . -
pubmed
Among the early cellular events occurring in
zinc - treated astroglia , strong activation of
p38 MAPK and
JNK was evident . -
pubmed
Although inhibitors of
p38 (
SB203580 and
SB202190 ) or
JNK (
SP600125 ) did not protect
zinc - insulted astroglia from cell
death , the
p38 inhibitors , but not the
JNK inhibitor , suppressed
actin filament and cell morphology disruption . -
pubmed
The
Ca ( 2 + ) ionophore ,
A23187 , also suppressed
actin filament and cell morphology disruption , but not cell
death , of
zinc - insulted astroglia . -
pubmed
However ,
A23187 did not inhibit
p38 MAPK activation in
zinc - treated astroglia . -
pubmed
Together these results suggest that
zinc influx in astroglia results in rapid loss of the morphologic integrity via mechanisms regulated by
p38 kinase and/or
Ca ( 2 + ) signaling . -
pubmed
The core electron temperature ( T(e0 ) ) of neutral beam heated plasmas is determined in TJ -
II stellarator by using soft x ray detectors with
beryllium filters of different thickness , based on the method known as the foil absorption technique . -
pubmed
Barium swallow and
CT scan of the chest were inconclusive . -
pubmed
To evaluate chemical
oxygen demand ( COD ) removal efficiency and granule formation of the novel reactor (
R1 ) , in comparison a conventional
SBAR ( R2 ) was operated under similar conditions during the experimental period . -
pubmed
Experiments were done at different organic loading rates ( OLRs ) ranging from 0.6 - 4.5 kg
COD / m3.d for
R1 and from 0.72 - 5.4 kg
COD / m3.d for
R2 . -
pubmed
In the second part of the experiment , the efficiency of removal of a toxic substance by aerobic granules was investigated using
aniline as a
carbon source with a concentration in the range 1.2 - 6.6 kg
COD / m3.d and 1.44 - 7.92 kg COD / m3.d in
R1 and
R2 , respectively . -
pubmed
Increased
SIRT1 expression in HUVECs by
syringaresinol treatment delayed cellular senescence and improved various markers of endothelial functions in a
FOXO3 dependent manner . -
pubmed
The
MTT and
wound - healing assays showed that the
TF - siRNA substantially inhibited the proliferation and migration of HUVECs . -
pubmed
Catabolism of
triglyceride - rich lipoproteins creates free
fatty acids that are readily taken up by endothelial cells , and , likely through the action of
acyl - CoA synthetases , exacerbate inflammatory processes . -
pubmed
A novel hollow urchin - like
alpha - FeOOH hierarchical structure was fabricated via a one -
pot hydrothermal procedure without using any templates , surfactants or organic raw materials . -
pubmed
Hospitalization , use of
sirolimus , low
hemoglobin level , and use of
renin - angiotensin system inhibitors were independently associated with these events . -
pubmed
Conversely , over the 3 - year study , the number of positive
Epstein - Barr virus viremias over the number of
Epstein - Barr virus polymerase chain reactions performed was significantly higher in the
belatacept group ( 76 % vs 50 % ; P = .047 ) . -
pubmed
There was a greater age- , sex- and ethnicity - adjusted odds of
AN among those :
overweight ( 3.98 ; 2.10 , 7.55 ) or
obese ( 8.31 ; 3.84 , 18.00 ) versus normal
BMI ; centrally
obese ( 4.72 ; 2.65 , 8.43 ) ; with history of
hypertension (
2.19 ; 1.27 , 3.79 ) or history of
hypercholesterolemia ( 1.72 ; 1.02 , 2.90 ) , but there was no evidence of this demographic - adjusted association ( p & gt ; 0.4 ) between
AN and history of previous
MI or
CVA , family history of
T2DM ,
T2DM treatment regimen , duration of
T2DM or random blood
glucose . -
pubmed
Opening and closing of the
cystic fibrosis transmembrane conductance regulator are controlled by
ATP binding and hydrolysis by the cytoplasmic
nucleotide - binding domains . -
pubmed
Renal function was assessed by measurement of
sCysC levels and
albuminuria ( calculated as the urine
albumin - to -
creatinine ratio ( uACR ) ) . -
pubmed
The presence of
EMH was evaluated based on steady - state free precession sequences ; cardiac and liver
iron overloads were quantified using a multiecho
T2 * approach ; cardiac function parameters and pulmonary diameter were quantified using the steady - state free precession sequences ; and
myocardial fibrosis was evaluated using the late
gadolinium enhancement technique . -
pubmed
Spiral Bragg grating waveguides for
TM mode
silicon photonics . -
pubmed
We demonstrate spiral Bragg grating waveguides ( BGWs ) on the
silicon - on - insulator ( SOI ) platform for the fundamental transverse magnetic (
TM ) mode . -
pubmed
Cell cycling and apoptosis were assessed by
propidium iodide (
PI ) and
annexin V -
FITC /
PI flow cytometry , respectively . -
pubmed
In this study we characterize the anti -
glioma effect of
VCN-01 , an improved
hyaluronidase - armed
pRB - pathway - selective oncolytic adenovirus that has proven safe and effective in the treatment of several
solid tumors . -
pubmed
METHODS : Six reagents Clearfil(TM )
SE Bond primer ( SP ) , Clearfil(TM )
SE Bond adhesive ( SA),Clearfil(TM ) Protect Bond primer (
PP ) , which contained antibacterial monomer
methacryloyloxydodecylpyridinium bromide (
MDPB ) , ClearfilTM Protect Bond adhesive (
PA ) , positive control
chlorhexidine acetate [
CHX , 1 % ( mass fraction ) ] , and negative control
phosphate buffer solution (
PBS ) were selected . -
pubmed
mutans ( P<0.05 ) ; SP and
PP showed stronger antibacterial effect than
PA ,
SA and
CHX ( P<0.05 ) . -
pubmed
CHX , SP and
PP presented inhibition zones , while
PBS ,
SA and
PA did not . -
pubmed
Compared with
PBS , the
CHX , SP and
PP could lower the ratio of the live to dead bacteria significantly ( P<0.05 ) . -
pubmed
However , systemic
letrozole treatment did not augment the stimulus - evoked release of the neuropeptide
calcitonin gene - related peptide (
CGRP ) from spinal cord slices , suggesting that the enhanced nociceptive responses were not secondary to an increase in peptide release from sensory endings in the spinal cord . -
pubmed
Contribution of
Matrix Metallopeptidase-1 Genotypes , Smoking ,
Alcohol Drinking and Areca Chewing to
Nasopharyngeal Carcinoma Susceptibility . -
pubmed
In addition , individuals carrying the 1 G allele at
MMP-1 promoter -1607 were less susceptible to
NPC ( OR=0.73 ; 95%CI=0.59 to 0.96 , p=0.0418 ) after adjustment for age , gender , cigarette ,
alcohol and areca consumption . -
pubmed
CONCLUSION : The 1G/1 G genotype of
MMP-1 promoter -1607 may independently have a protective effect on
NPC risk , without interaction with behavioral factors , including cigarette ,
alcohol and areca consumption . -
pubmed
The rapid uptake of
68Ga prostate - specific membrane antigen
HBED - CC positron emission tomography (
PSMA PET ) imaging for
prostate cancer staging has led to concerns regarding its specificity , with uptake in both malignant and nonmalignant tissues . -
pubmed
But also
today ,
EPP is still a valuable option in selected cases and the entire treatment concept requires a nuanced view , which will be discussed in this review . -
pubmed
Selectivities of 70 - 80 % for
C2 and C3 products with a
hydrocarbon ratio of C>=2 /
CH4 significantly greater than 100 have been observed with several additives . -
pubmed
The complete repertoire of endothelial functions elicited by
FGD5 , a
guanine nucleotide exchange factor activating the Rho GTPase
Cdc42 , has yet to be elucidated . -
pubmed
Furthermore , depletion of
FGD5 resulted in accelerated
VEGFR2 degradation , which was reverted by
lactacystin - mediated proteasomal inhibition . -
pubmed
Editorial
SI :
Octamethylcyclotetrasiloxane . -
pubmed
Retigabine (
RTG ) is a first - in - class antiepileptic drug that suppresses neuronal excitability through the activation of voltage - gated
KCNQ2 - 5 potassium channels . -
pubmed
To elucidate how the
retigabine binding site is coupled to changes in voltage sensing , we used voltage -
clamp fluorometry to track conformational changes of the
KCNQ3 voltage - sensing domains ( VSDs ) in response to voltage ,
retigabine , and
PIP2 . -
pubmed
We identify a polybasic motif in the proximal C terminus of
retigabine - sensitive
KCNQ channels that contributes to VSD - pore coupling via
PIP2 , and thereby influences the unique gating effects of
retigabine . -
pubmed
The synthesis of
phosphatidylcholine ( PC ) , the most abundant cellular phospholipid , occurs redundantly via the
ER - localized Kennedy pathway and a pathway that traverses the
ER and mitochondria via membrane contact sites . -
pubmed
The basis of the
ER - mitochondrial PC synthesis pathway is the exclusive mitochondrial localization of a key pathway enzyme ,
phosphatidylserine decarboxylase Psd1 , which generates
phosphatidylethanolamine (
PE ) . -
pubmed
Renal cells with upregulated miR-146 had lower plasma levels of blood
urea nitrogen (
BUN ) and
creatinine , decreased apoptosis and active
caspase-3 protein expressions . -
pubmed
In the former ,
P2Y and possibly some
P2X receptors - ion channels regulate
insulin secretion , but it is still debated whether excessive
ATP can via
P2X receptors impair beta - cell function directly or whether cell damage is due to an excessive systemic release of
cytokines . -
pubmed
The Detection of
Uterine Leiomyoma (
Fibroid )
Calcifications on
18F - NaF PET /
CT . -
pubmed
We are presenting a calcified
uterine leiomyoma in a 61 - year - old woman as incidentally detected on
F - NaF PET /
CT scanning . -
pubmed
The mature
XynC has 454
amino acid residues and a calculated molecular weight of 49 240 . -
pubmed
It liberates a set of
oligosaccharides , which have been detected by thin - layer chromatography , thus indicating that it is an endo - acting
xylanase . -
pubmed
Then to catalyze the
zeolite Y
wash - coated monolith with the
cobalt precursor , the dip coating technique was used . -
pubmed
Though Pusa
Basmati1 (
PB1 ) showed higher tolerance to
As , IR64 overcomes
As toxicity more efficiently than
PB1 in the presence of
Se , which highlights that
IR64 is a better performer in the presence of
Se . -
pubmed
Our EGA - IAMS results obtained from the thermal decomposition of
vitamin C were compared with a previous study employing pyrolysis - gas chromatography - mass spectrometry ( Pyr -
GC -
MS ) . -
pubmed
The observed quantitative and qualitative differences of the pyrolysis products obtained by the two techniques ( EGA - IAMS vs.
Pyr -
GC -
MS ) are in part due to the difference in transportation time of the products out of the pyrolysis chamber . -
pubmed
PDH is central to metabolism because it steers most of the
glucose - derived flux into the
TCA cycle . -
pubmed
Here , we provide evidence that the RNA editing enzyme
adenosine deaminase acting on
RNA 2 (
ADAR2 ) is mislocalized in
C9orf72 repeat expansion mediated
ALS /
FTD . -
pubmed
ADAR2 is responsible for
adenosine ( A ) to
inosine ( I ) editing of double - stranded RNA , and its function has been shown to be essential for survival . -
pubmed
The clinical effectiveness rate in the
TEC group was significantly higher than that in the
CEF group ( p<0.05 ) . -
pubmed
There was no difference in the pathology grade between the two groups ( p>0.05 ) ; however , the pathological effective rate in the
TEC group was significantly higher than that in the
CEF group ( p<0.05 ) . -
pubmed
While there was no difference in the relative expression of
ANXA3 between the two groups before chemotherapy , the relative expression of
ANXA3 in the
TEC group was lower than that in the
CEF group after
NAC ( p<0.05 ) . -
pubmed
In this study , we investigated the role of
TRIM33 in myeloid cells during dextran sulfate
sodium ( DSS)-induced
colitis . -
pubmed
Methotrexate (
MTX ) was administered in a 5 - day regimen : 0.3 - 0.5mg / kg intravenously (
IV ) daily for 5 days every 2 weeks ( maximum 25 mg per dose ) . -
pubmed
Actinomycin D (
ActD ) was administered 1.25mg / m2 pulsed
IV every 2 weeks . -
pubmed
Up - regulation of
sirtuin 1 induced by
resveratrol also diminished the
TNF - alpha - induced inflammatory response in BMSCs . -
pubmed
Thus ,
betaOHB may regulate epigenetics by modulating
HDACs . -
pubmed
The aim of this study was to investigate whether
betaOHB enhances
cisplatin - induced apoptosis in
hepatocellular carcinoma (
HCC ) cells by modulating activity and/or expression of
HDACs . -
pubmed
We found that
betaOHB significantly enhanced
cisplatin - induced apoptosis and cleavage of
caspase-3 and - 8 in
HCC cells . -
pubmed
Acid sphingomyelinase (
ASM ) is a lysosomal hydrolase that degrades
sphingomyelin into
ceramide and
phosphocholine . -
pubmed
The
Na / H exchange regulatory factor 1 (
NHERF1 ) , a member of a PDZ scaffolding protein family , was first identified as an organizer of membrane - bound protein complexes composed of
hormone receptors , signal transduction pathways , and
electrolyte and mineral transporters and channels . -
pubmed
, 0 , 120 , and 240 mg kg-1 of soil ( for
pot experiment ) and 0 , 300 , and 600 kg ha-1 ( for field experiment ) regarded as LSe , MSe and
HSe , respectively and two
Si levels i.e. -
pubmed
, 0 and 60 mg kg-1 of soil ( for
pot experiment ) and 0 and 150 kg ha-1 ( for field experiment ) regarded as -
Si and +
Si , respectively . -
pubmed
Using different behavioral tests , we found that a single intravenous injection of
methylene blue (
MB , 1 mg / kg ) 30 min after the
injury reduced the impairment of the motor functions of the limbs from 7 to 120 days after
TBI . -
pubmed
We observed that
protease complexed with
Cyclocytidine hydrochloride ( anti - viral and anti -
cancer ) ,
Trifluridine ( anti - viral ) ,
Adonitol , and
Meropenem ( anti - bacterial ) , and
Penciclovir ( anti - viral ) bound with a good docking score ranging from -6.8 to -5.1 ( Kcal / mol ) . -
pubmed
Further ,
NSP10 /
NSP16 methyltransferase complexed with
Telbivudine ,
Oxytetracycline dihydrate ( anti - viral ) ,
Methylgallate ( anti - malarial ) ,
2 - deoxyglucose and
Daphnetin ( anti -
cancer ) from the docking score of -7.0 to -5.7 ( Kcal / mol ) . -
pubmed
The EDC values correlated well (
R2 = 0.93 ) with the 24 h
Cl2 demand of the model compounds ( except for
phenol and
tannic acid ) . -
pubmed
Our results showed that
azosemide and
torasemide caused a significant reduction in
brain natriuretic peptide (
BNP ) level . -
pubmed
Torasemide also caused a significant decrease in
collagen volume fraction ( CVF ) and
edema . -
pubmed
We showed that
KY1044 induced sustained depletion of ICOShigh T cells but was also associated with increased secretion of pro - inflammatory
cytokines from ICOSlow TEFF cells . -
pubmed
KY1044 demonstrated monotherapy antitumor efficacy and improved anti -
PD - L1 efficacy . -
pubmed
In summary , we demonstrated that using
KY1044 , one can exploit the differential expression of
ICOS on T cell subtypes to improve the intratumoral immune contexture and restore an antitumor immune response . -
pubmed
LRAT ,
retinoic acid ,
renin angiotensin system (
RAS ) biomarkers , and the structure and function of the heart for SHRs were measured . -
pubmed
Compared with
VAN - treated SHRs ,
LRAT ,
retinoic acid receptor alpha (
RARalpha ) ,
ACE2 , and
Ang ( 1 - 7 ) protein expression levels were decreased , while
ACE and
AT1R expression levels were increased in VAD SHRs . -
pubmed
Catalytic Synthesis of
Potassium Acyltrifluoroborates (
KATs ) from
Boronic Acids and the
Thioimidate KAT Transfer Reagent . -
pubmed
We report the synthesis of
potassium acyltrifluoroborates (
KATs ) by a
palladium - catalyzed cross - coupling of
boronic acids and the
thioimidate KAT transfer reagent . -
pubmed
The combination of widely available
aryl - and vinylboronic acids with commercially available
thioimidate 1 using catalytic Pd
II and a
Cu II additive enables the preparation of KATs in high yields and with good functional group tolerance . -
pubmed
The cross - coupling can be telescoped into the one -
pot synthesis of
amides and a -
aminotrifluoroborates by exploiting the unique chemistry of KATs and their
trifluoroborate iminium (
TIM ) derivatives . -
pubmed
One hundred eighty trough levels were first measured , and their median value was
16.2 mug / mL ( range , 2.3 - 100 mug / mL ) and the median interval between initial
teicoplanin injection and 1st trough level was 96.5 hours ( range 47.6 - 179.3 hours ) . -
pubmed
Deciphering the Molecular Details of the
Lipoxin Formation Mechanism in the
5 ( S ) , 15 ( S )- DiHpETE Biosynthetic Pathway Catalyzed by
Reticulocyte 15 - Lipoxygenase-1 . -
pubmed
The biosynthesis of
lipoxins is catalyzed by several
lipoxygenases (
LOXs ) from
arachidonic acid . -
pubmed
In this paper , we have combined molecular dynamics ( MD ) simulations and quantum mechanics / molecular mechanics ( QM / MM ) calculations to analyze how reticulocyte
15 - LOX-1 catalyzes the production of
lipoxins from
5 ( S ) , 15 ( S )- diHpETE . -
pubmed
Conversely , the oxygenation of
5 ( S ) , 15 ( S )- diHpETE at C14 is feasible because there is an open channel connecting the
protein surface with this
carbon atom , and the energy barrier for
oxygen addition through this channel is small . -
pubmed
The analysis of the following steps of this mechanism , leading to the corresponding
hydroperoxide at the 15 -
LOX-1 active site , indicates that the oxygenation mechanism will lead to the formation of
lipoxinB4 after the final action of a
reductase . -
pubmed
In contrast , our calculations are in agreement with experiments that
lipoxinA4 can not derive from
5 ( S ) , 15 ( S )- diHpETE by either of the two proposed mechanisms and that
5 ( S ) , 15 ( S )- diHETE is not an intermediate of
lipoxin biosynthesis catalyzed by
15 - LOX-1 . -
pubmed
Pharmacogenetic Variants in the
DPYD and
TYMS Genes are Clinically Significant Predictors of
Fluoropyrimidine Toxicity : Are We Ready for Use in our Clinical Practice . -
pubmed
Dantrolene Potentiates the Antineoplastic Effect of
Sorafenib in
Hepatocellular Carcinoma via Targeting
Ca + 2 /
PI3 K Signaling Pathway . -
pubmed
OBJECTIVE : This study was conducted to evaluate the role of
dantrolene as a possible antineoplastic agent in
HCC , and in chemo - sensitization of
sorafenib via targeting
Ca + 2 /
PI3 K pathway . -
pubmed
RESULTS :
Dantrolene exhibited synergistic effect when used in combination with
sorafenib compared to either drug alone ( p < 0.05 ) through decreasing
p53 ,
VEGF ,
MMP-9 ,
Cyclin D1 , and
Ki-67 . -
pubmed
In addition ,
dantrolene was evidenced to have an inhibitory effect on
Ca + 2 /
PI3 K pathway that mediates its antineoplastic action when used alone or in combination with
sorafenib . -
pubmed
CONCLUSION :
Dantrolene exerted antineoplastic effect as well as augmented
sorafenib antineoplastic activity via intervention of
Ca + 2 /
PI3 K pathway , manifested by ameliorating angiogenesis , apoptosis , proliferation and
metastasis . -
pubmed
Although
insulin , by itself , had a stimulatory effect on
[ 125I ] UdR incorporation in cultured prostate , the magnitude of the response did not differ in the 6- and 26 - week - old prostate tissue . -
pubmed
This spontaneous surge in activity during culture tended to mask the stimulatory effects of
insulin and
testosterone at concentrations of 3 mug / ml and 10(-7 ) mol / l respectively . -
pubmed
They do not modify the
prostate 5alpha - reductase activity ; however , they do inhibit the binding of
androgens to their receptors . -
pubmed
All these fractions , except one
casein kinase of the cytosol , have been studied with respect to protein and
nucleotide specificity , effect of salts and of
cyclic nucleotides , sedimentation , etc . -
pubmed
The content of
CGRP-2 ,
substance P , and
somatostatin in
adenomas correlated directly with that of
parathyroid hormone . -
pubmed
In addition , concentrations of
VIP versus
substance P and
somatostatin versus
CGRP-2 in
adenomas were directly correlated . -
pubmed
In
hyperplasias , content of
substance P correlated directly with that of
gastrin - releasing peptide . -
pubmed
Thus ,
dopamine effectively inhibited
PRL secretion during the later lactational stage , but could not do so during the earlier stage when there were mechanisms other than
dopamine stimulating
PRL secretion . -
pubmed
The mechanism of ovulation blockade during lactation thus seems to shift from being
progesterone independent to
progesterone dependent at a similar period when the neuroendocrine control of
PRL secretion shifts from
dopamine independent to dependent . -
pubmed
Endonuclease V recognizes ultraviolet radiation ( UVR)-induced
pyrimidine dimers and produces single - strand breaks adjacent to the dimers . -
pubmed
When removal of UVR - induced photoproducts was initiated by endogenous excision repair mechanisms , an average of 38
nucleotides were replaced per dimer removed , as determined by
bromouracil photolysis ;
denV - initiated excision repair , on the other hand , resulted in removal of an average of 6
nucleotides per dimer repaired . -
pubmed
The enhanced
pyrimidine dimer repair capabilities conferred by
denV gene expression did not appear to improve post - UVR survival . -
pubmed
Internally trapped
cytochrome c can be reduced by the membrane - permeable reductants
2,3,5,6 - tetramethyl - p - phenylenediamine (
DAD ) or
N , N , N ' , N '- tetramethyl - p - phenylenediamine (
TMPD ) . -
pubmed
Respiration on internal
cytochrome c generated a membrane potential of 53 mV ( positive inside ) and a pH gradient of 0.2 ( acid inside ) as monitored by the optical probes
oxonol V and
pyranine , respectively . -
pubmed
The steady - state internal acidification induced by turnover of the internally facing enzyme is probably maintained by both
cytochrome oxidase proton translocation and a
TMPD+/H+ antiport present in these vesicles [ Cooper , C. E. , & amp ; Nicholls , P. ( 1987 ) FEBS . -
pubmed
The heart to lung ratio both at
rest and exercise for
99mTc - MIBI is significantly higher than
201Tl ( P less than 0.01 and less than 0.001 respectively ) . -
pubmed
Analysis with
restriction enzymes shows that both
methyl and
ethyl groups influence the restriction patterns of the enzymes
HpaII and
MspI that recognize specific endogenous DNA methylation . -
pubmed
Intravenous infusion of natural peptide YY at 1 and 0.2 microgram / kg / hr caused 60 and 40 % inhibition of
secretin- and cholecystokinin - stimulated secretion , respectively , and 40 - 50 and 20 - 40 % inhibition of intraduodenal
oleate stimulated secretion , respectively . -
pubmed
A significant but transient decrease in the plasma cholecystokinin level was observed in response to
peptide YY under
oleate stimulation . -
pubmed
The levels of both
factor VIII : C and
factor VIIIR :
Ag increased , the former less than the latter resulting in a rise of the
FVIIIR :
Ag /
FVIII : C ratio . -
pubmed
FVIIIR :
Ag /
FVIII : C ratio was increased both in mild ( n = 7 ) and severe ( n = 9 )
preeclampsia , whereas
beta - thromboglobulin was increased and
AT III was decreased only in severe
preeclampsia . -
pubmed
Platelet count and lifespan , plasma and serum
thromboxane B2 as well as
FVIII : C were normal in severe
preeclampsia . -
pubmed
Stable
acyl - derivatives of
tissue - type plasminogen activator . -
pubmed
Injections of
morphine - HCl ( MP ) ( 25 , 50 , 100 and 150 micrograms / g ) were administered and the DR was examined at 2 , 7.5 , 15 , 30 , 45 and 75 min
post - injection . -
pubmed
( b ) A 100 micrograms / g dose of MP caused a 50 % response inhibition with an injection -
shock interval of 30 minutes , but no inhibition occurred when the same dose was administered with 1.6 micrograms / g of
naloxone -
HCl , suggesting that MP acts through an
opiate receptor . -
pubmed
After training , each patient exceeded FVC with one or more resistors ; " optimum " FVCPEP was 124 + /- 16 % FVC at a
SEP of 61 + /- 21 cm
H2O. -
pubmed
Hypoxia induced a decline in cardiac contractile force , a decrease in myocardial high - energy
phosphates and a release of
ATP metabolites and
creatine phosphokinase from the perfused heart . -
pubmed
Upon reoxygenation the rate of release of
ATP metabolites subsided , but no appreciable recovery of the loss of contractile force and the reduction of myocardial
ATP content was seen , and the release of
creatine phosphokinase was increased further . -
pubmed
Both analyses showed low activity of the enzymes studied and a decrease of the
androgen level in
E2 treated cultures . -
pubmed
When the dose of
estradiol preceded
LH addition the
androgen response to
LH was attenuated . -
pubmed
Recent studies suggest keyroles for terminal components of complement system , products of
lipoxygenase pathway of
arachidonic acid and
oxygen radicals - all of which are not readily influenced by available therapeutic modulaties . -
pubmed
The effect of
dexamethasone on
corneal neovascularization induced by
epidermal growth factor . -
pubmed
The divalent cation - selective ionophore
A23187 has been used to characterize cytoplasmic
Ca and
Mg buffering ,
Ca2 + -pump parameters and the properties of a
Ca2 + - activated K +- channel in intact red cells . -
pubmed
Phosphatidic acid ,
phosphatidyl ethanolamine ,
phosphatidyl serine ,
phosphatidyl inositol ,
phosphatidyl glycerol and
cardiolipin were found to activate greatly the
cathepsin D. -
pubmed
The
ABH " blood - group "
antigens are present on differentiated epidermal cell surfaces and identify the grafted epidermis according to the blood groups of the donor . -
pubmed
3 The SSPC of
PL before exposure to
DPH varied from 1.2 to 4.4 microgram ml-1 ( mean + /- s.e.mean 2.7 + /- 0.13 ) , and following exposure from 1.1 to 3.8 microgram ml-1 ( mean + /- s.e.mean 2.2 + /- 0.13 ) . -
pubmed
4 SSPCs of AA and of
PL decreased significantly during
DPH administration ( P less than 0.01 for AA , P less than 0.001 for
PL ) . -
pubmed
6 Similarity was observed between the changes in plasma levels of
AA and
PL following
DPH ingestion . -
pubmed
Pericytes and endothelial cells were studied by immunohistochemistry ,
lectin labeling , electron microscopy ,
3H - thymidine labeling followed by autoradiography , and time - lapse video microscopy . -
pubmed
We report here that there is an increase of
tyrosine kinase activity immunoprecipitable with
anti - phosphotyrosine antibodies specifically in response to
calcium -- and a number of other divalent cations -- within 2 min of exposure . -
pubmed
A second
tyrosine kinase activity is induced in response to both
calcium and
TPA , and has been identified as
fyn , a
nonreceptor tyrosine kinase of the
src family . -
pubmed
fyn activation is induced in keratinocytes within 6 hr of
calcium exposure , but already within 2 min of
TPA treatment . -
pubmed
Cortactin , a
p80 - 85 substrate of
src- and
fyn - related kinases that localizes with
actin at cell adhesion sites , is increasingly
tyrosine phosphorylated in
calcium- and
TPA - induced differentiation , with a time course which parallels that of
fyn activation . -
pubmed
Autoregulation of
glucocorticoid receptor (
GR ) concentration in vivo may be an important determinant of
steroid sensitivity . -
pubmed
Receptor and
TAT message levels were determined by quantitative Northern hybridization . -
pubmed
Methylprednisolone plasma kinetics showed a half - life of 0.6 h. Receptor occupancy occurred rapidly and
cytosolic GR reappeared over 2 - 12 h.
TAT activity rose between 2 and 6 h and then dissipated . -
pubmed
In contrast ,
TAT message induction occurred with a sharp peak ; maximal induction occurred between 5 - 6 h and return to baseline at approx . -
pubmed
This study shows that unlike
TAT induction ,
GR message repression in vivo does not require continual presence of hormone . -
pubmed
Activators of
protein kinase C mimic
serotonin - induced modulation of a voltage - dependent
potassium current in pleural sensory neurons of Aplysia . -
pubmed
In the pleural mechanoafferent sensory neurons of Aplysia ,
serotonin ( 5 - HT)-induced spike broadening consists of at least two components : a
cAMP and
protein kinase A (
PKA)-dependent , rapidly developing component and a
protein kinase C (
PKC)-dependent , slowly developing component . -
pubmed
Voltage -
clamp experiments were conducted to identify currents that are modulated by
PKC and thus may contribute to the slowly developing component of
5 - HT - induced spike broadening . -
pubmed
We compared the effects of
phorbol esters , activators of
PKC , on membrane currents with those of 5 - HT . -
pubmed
Previous work has shown that these effects represent , in part , the modulation of a large , voltage - dependent
K + current (
IK , V ) by 5 - HT . -
pubmed
Active
phorbol esters mimicked some of the actions of
5 - HT on membrane currents in that they slowed activation and inactivation kinetics of current responses to voltage -
clamp pulses more positive than 0 mV. -
pubmed
Because inactive
phorbols did not mimic the actions of
5 - HT , the effects of active
phorbol esters appeared to be
PKC specific . -
pubmed
In addition , preexposure of the sensory neurons to active
phorbol esters appeared to occlude the modulatory actions of
5 - HT on
IK , V. Thus it is likely that modulation of
IK , V by
5 - HT is mediated , at lease in part , by
PKC . -
pubmed
To further characterize which currents were modulated by
PKC , low concentrations of
tetraethylammonium (
TEA , 2 mM ) were used to block
Ca ( 2 +)-activated
K + current (
IK ,
Ca ) . -
pubmed
Low
TEA partially blocked the
phorbol ester - induced increase of the outward current at the end of voltage -
clamp pulses . -
pubmed
These results agreed with previous reports that activation of
PKC enhanced a fast component of
IK ,
Ca in these sensory neurons . -
pubmed
Low
TEA , however , did not affect
phorbol ester - induced decrease of the outward current at the beginning of
pulse , where the predominant current is
IK , V , which is less sensitive to TEA. -
pubmed
In addition , biochemical data including fasting plasma
glucose ,
hemoglobin ( HD)Alc ,
cholesterol ,
triacylglycerol , high- and
low - density lipoprotein cholesterol ,
uric acid , blood
urea nitrogen (
BUN ) and
creatinine (
Cr ) were studied . -
pubmed
Moreover ,
topoisomerase activities were not affected by
docetaxel . -
pubmed
The
acetylcholine - related enzymes
acetylcholinesterase (
AChE ) and
butyrylcholinesterase (
BuChE ) were measured in plasma , erythrocytes , platelets and lymphocytes . -
pubmed
Electron microscopic immunolocalization of
GABA and
glutamic acid decarboxylase (
GAD ) in cerebellar capillaries and their microenvironment . -
pubmed
The objective of the present study was to determine the influence of
bombesin , a potent mitogen , supplemented alone or in combination with
insulin ,
transferrin and/or
EGF . -
pubmed
When compared with 5 day controls ( L-15 medium only ) ,
bombesin generated a maximal though weak effect on DNA synthesis at a concentration of 0.3 nM , corresponding to a stimulation index (
SI ) of 22 % . -
pubmed
When combined with either
transferrin or
EGF , or with
transferrin plus
EGF ,
bombesin did not alter the
SI of individual factors . -
pubmed
Insulin , in turn , greatly increased DNA synthesis (
SI = 169 % ) , while
bombesin strongly potentiated this effect (
SI = 275 % ) . -
pubmed
When added as a third factor ,
bombesin further potentiated the effectiveness (
SI = 338 % ) of the combination
insulin plus
transferrin . -
pubmed
In a test of working memory using a three - panel runway task , the
5 - HT1A receptor agonist ,
8 - hydroxy-2 -( di - n - propylamino ) tetralin (
8 - OH - DPAT ) , at 1.0 mg / kg i.p . -
pubmed
The effect of intrahippocampal
8 - OH - DPAT ( 10 micrograms / side ) on working memory was blocked by the
5 - HT1A receptor antagonist , ( -)-
propranolol , at 5 mg / kg i.p . -
pubmed
S-100 - positive neurons are distinguished by spontaneous discharge activity in a beating or bursting mode and a prominent
Ca ( 2 +)-activated
potassium outward current . -
pubmed
The intent of this retrospective study was to perform a risk - benefit analysis between treatment strategies with bolus
steroids only versus
anti - thymocyte globulins ( RATG ; 1.5 mg / kg q 4 days ) . -
pubmed
Heparin inhibitable
lectins are physiologically important because of their interactions with extracellular matrix and with other cell surface
glycoconjugates . -
pubmed
When
proteins were loaded on to a
phosphate - buffered saline (
PBS ) equilibrated column and eluted with salt gradient , only one
lectin peak was obtained . -
pubmed
Lectin activity was stimulated by
Mn ++ ;
amino acid composition was different from other known
lectins . -
pubmed
The
lectin was particularly rich in
acidic amino acids . -
pubmed
Hepatic
cytochrome P-450 enzymes mediate at least two important biotransformation pathways of
codeine and
ethylmorphine starting with either N - demethylation or O - dealkylation , producing polar metabolites which are then subsequently glucuronidated . -
pubmed
Butyrate and colonocytes :
Bax to the front . -
pubmed
A truncated version of the gene which lacks 381 bp of 5'-sequence also has
pullulanase activity and it appears that the
amino - terminal portion of the gene is not essential for either activity or thermostability . -
pubmed
Amino acid sequence comparisons with other published
amylases and
pullulanases showed that it possesses homology to the four key regions common to these enzymes . -
pubmed
The effect of age on
cholinesterase activity , the infusion rate depressing twitch tension by 90 % ( IR90 ) , C90 ,
Cl ( trans - trans ) , and
Cl ( cis - trans ) was determined using linear regression . -
pubmed
mGluR activation reveals a tonic
NMDA component in inflammatory
hyperalgesia . -
pubmed
After auto - radiographic in situ hybridization , counting
silver grains over cells was used to quantitate and compare levels of expression of the
N - methyl - D - aspartate receptor-2 subunits . -
pubmed
Thus , these data provide clear functional support for the hypothesis that the C3H / HeJ defect exerts a dominant negative effect on LPS sensitivity ; however , expression of a normal
Toll - like receptor 4 molecule is apparently not required . -
pubmed
Labeling of adjacent sections for
enkephalin or
substance P showed that the dynorphinergic terminals overlapped those for
substance P in the medial half of the internal pallidal segment , but showed only slight overlap with enkephalinergic terminals in the external pallidal segment . -
pubmed
Telmisartan did not have consistent effects on either plasma
norepinephrine or plasma
atrial natriuretic peptide levels , although it did cause significant increases in both
plasma renin activity and
angiotensin II levels at higher doses . -
pubmed
CONCLUSIONS : The acute administration of the
angiotensin II antagonist
telmisartan was associated with significant dose - dependent
reductions in systemic arterial blood pressure and pulmonary pressures . -
pubmed
Although
L - N ( G )- nitroarginine methylester (
L - NAME ) ( 100 microM , n=8 ) had no effect ,
superoxide dismutase ( 10 U ml(-1))+
catalase ( 100 U ml(-1 ) ) increased the number of
ventricular ectopic beats from 91+/-32 to 286+/-83 ( n=5 ) and augmented the incidence of irreversible
ventricular fibrillation from 0 % to 60 % . -
pubmed
The finding that some
B and T cell epitopes were localized to regions with
amino acid substitutions may account for the putative differential responsiveness to this antigen in
tuberculosis and
leprosy . -
pubmed
A blood pressure decrease was observed with both
L - arginine - rich diets ( Diet 2 v 1 ,
SBP : -6.2 mm Hg [ 95 % CI : -0.5 to -11.8 ] ,
DBP : -5.0 mm Hg [ -2.8 to -7.2 ] ; Diet 3 v 1 ,
SBP : -6.2 mm Hg [ -1.8 to -10.5 ] , DBP : -6.8 mm Hg [ -3.0 to -10.6 ] ) . -
pubmed
Serum total
cholesterol ( P = .06 ) and
triglyceride ( P = .009 ) decreased and
HDL cholesterol increased ( P = .04 ) after Diet 2 , but not after Diet 3 . -
pubmed
The
HDMF levels determined in the wines studied using a stable isotope dilution assay ( SIDA ) method were consistent with the results found by sensory analysis and
GC / O ; i.e. -
pubmed
Exposure of HBECs of
asthmatic subjects to both 50 to 100 ppb
O3 and 200 to 400 ppb
NO2 significantly increased the release of
IL-8 ,
GM - CSF ,
RANTES , and
sICAM-1 from these cells after 24 hours of incubation . -
pubmed
However , 50 to 100 ppb
O3 and 200 to 400 ppb
NO2 led to a significant increase in release of only
IL-8 and
sICAM-1 from HBECs of nonasthmatic subjects after 24 hours ' incubation . -
pubmed
CONCLUSION : These results suggest that
O3 and
NO2 may modulate
airway diseases , such as
asthma , by increasing the release of
inflammatory mediators from bronchial epithelial cells and that the cells of
asthmatic subjects may be more susceptible to the adverse effects of these pollutants . -
pubmed
In contrast , at 1 week after the
STZ injection only plasma
ET-1 and
glucose levels were elevated , suggesting that the
hyperglycaemia might trigger the excess production of
ET-1 . -
pubmed
Incubation with high
glucose promoted the release of
ET-1 from the isolated mesenteric arterial bed . -
pubmed
PvuII and XbaI restriction fragment length polymorphisms ( RFLPs ) for the
estrogen receptor gene (
ERG ) and its relation to bone mineral density (
BMD ) were examined in 454 postmenopausal Chinese women , aged 55 - 79 years . -
pubmed
Two important factors have been taken into account in compiling these recommendations : ( 1 ) the lack of level I evidence to convincingly demonstrate the value of
HER2 as a predictive marker for resistance or sensitivity to chemo- and hormonal therapy , and ( 2 ) the recently published pivotal phase
II and III trial data proving the efficacy of
trastuzumab as a single agent and in combination with chemotherapy in
HER2 - positive metastatic
breast cancer . -
pubmed
Because nonselective
cycloooxygenase (
COX ) inhibition attenuated
anorexia after lipopolysaccharide ( LPS ) administration , we tested the ability of
resveratrol ( 2.5 , 10 , and 40 mg / kg ) and
NS-398 ( 2.5 , 10 , and 40 mg / kg ) , selective inhibitors of the two
COX isoforms
COX-1 and - 2 , respectively , to attenuate LPS ( 100 microg / kg ip)-induced
anorexia . -
pubmed
Because
prostaglandin ( PG ) E ( 2 ) is considered the major metabolite synthesized by
COX , we measured plasma and cerebrospinal fluid (
CSF )
PGE ( 2 ) levels after LPS administration . -
pubmed
LPS induced a time - dependent increase of
PGE ( 2 ) in
CSF but not in plasma . -
pubmed
NS-398 ( 5 , 10 , and 40 mg / kg ) blocked the LPS - induced increase in
CSF PGE ( 2 ) , whereas
resveratrol ( 10 mg / kg ) did not . -
pubmed
These results support a role of
COX-2 in mediating the anorectic response to peripheral LPS and point at
PGE ( 2 ) as a potential neuromodulator involved in this response . -
pubmed
The main results can be summarized as follows : ( 1 ) urinary excretion of
adrenaline ,
noradrenaline and
cortisol was facilitated , and the amounts of urinary excretion were significantly higher when
TC were worn . -
pubmed
Heart rate was significantly higher in the
TC group ; ( 2 ) nocturnal urinary
melatonin excretion was significantly greater in the
TC group . -
pubmed
Functional
copper at the
acetyl - CoA synthase active site . -
pubmed
The bifunctional
CO dehydrogenase /
acetyl - CoA synthase (
CODH /
ACS ) plays a central role in the Wood - Ljungdahl pathway of autotrophic
CO ( 2 ) fixation . -
pubmed
Depletion of
copper correlates with reduction in
ACS activity and in intensity of the "
NiFeC " EPR signal without affecting either the activity or the
EPR spectroscopic properties associated with
CODH . -
pubmed
In contrast ,
Zn content is negatively correlated with
ACS activity without any apparent relationship to
CODH activity . -
pubmed
XAS studies are consistent with a distorted
Cu ( I )- S ( 3 ) site in the fully active
enzyme in solution . -
pubmed
These results indicate an essential and functional role for
copper in the
CODH /
ACS from acetogenic and methanogenic organisms . -
pubmed
8024 - 37 - 1 ) ( Major Component 79%-85 %
Curcumin ,
CAS No . -
pubmed
THE EFFECT OF
PROTEIN LEVEL AND
AMINO ACID BALANCE UPON THYROID ACTIVITY IN THE CHICK . -
pubmed
The cumulative incidence of severe ( grade III -
IV ) acute
GVHD and extensive chronic
GVHD was 4 % , 0 % , 44 % and 11 % , 8 % , 44 % in groups receiving
ATG in a dose of 8 mg / kg , 6 mg / kg , and 4 mg / kg , respectively ( severe acute
GVHD : p<0.001 ; extensive chronic
GVHD : p=0.05 ) . -
pubmed
CONCLUSION : Based on these findings , we recommend when
ATG is used in the setting of stem cell transplantation with ( partially )
TCD grafts from MUDs , to give a total dose of 6 to 8 mg / kg . -
pubmed
Both the activation of
DNases and chromatin fragmentation are suppressed in the presence of the
caspase 3 / 7 inhibitor
Ac - DEVD - CHO . -
pubmed
Topoisomerase I - mediated DNA cleavage as a guide to the development of antitumor agents derived from the marine alkaloid
lamellarin D :
triester derivatives incorporating
amino acid residues . -
pubmed
The marine
alkaloid lamellarin D (
LAM - D ) has been recently characterized as a potent poison of
human topoisomerase I endowed with remarkable cytotoxic activities against
tumor cells . -
pubmed
Two groups of
triester compounds incorporating various substituents on the three
phenolic OH at positions 8 , 14 and 20 of
6H -[ 1 ] benzopyrano [ 4 ' , 3 ' : 4,5 ] pyrrolo [ 2,1 - a ] isoquinolin-6 - one pentacyclic planar chromophore typical of the parent alkaloid were tested as
topoisomerase I inhibitors . -
pubmed
The
non - amino compounds in group A showed no activity against
topoisomerase I and were essentially non cytotoxic . -
pubmed
In sharp contrast , compounds in group B incorporating
amino acid residues strongly promoted DNA cleavage by
human topoisomerase I. -
pubmed
LAM - D derivatives tri - substituted with
leucine ,
valine ,
proline ,
phenylalanine or
alanine residues , or a related
amino side chain , stabilize
topoisomerase I - DNA complexes . -
pubmed
The DNA cleavage sites detected at T downward arrow G or C downward arrow G
dinucleotides with these molecules were identical to that of
LAM - D but slightly different from those seen with
camptothecin which stimulates
topoisomerase I - mediated cleavage at T downward arrow G only . -
pubmed
In the DNA relaxation and cleavage assays , the corresponding
Boc - protected compounds and the analogues of the non - planar
LAM-501 derivative lacking the 5 - 6 double bond in the
quinoline B - ring showed no effect on
topoisomerase I and were considerably less cytotoxic than the corresponding cationic compounds in the
LAM - D series . -
pubmed
LAM - D is the lead compound of a new promising family of antitumor agents targeting
topoisomerase I and the
amino acid derivatives appear to be excellent candidates for a preclinical development . -
pubmed
is likely but has to be confirmed ; ( ii ) in male
infertility ,
COH with
gonadotropins in conjunction with i.u.i . -
pubmed
improves the pregnancy rate but stimulation with
clomifene citrate appears to be less effective than
gonadotropins administration . -
pubmed
The
Akt /
PKB kinase is an important cell survival protein which is regulated by
D3 -
phosphoinositides . -
pubmed
[ Wide - spectrum
beta - lactamases : are they a serious problem
today in Spain ? ] -
pubmed
Exposure of cells to
ALA or its reduced form
dihydrolipoic acid (
DHLA ) for 24 h dose dependently increased
caspase-3 - like activity and was associated with DNA - fragmentation . -
pubmed
Increased mitochondrial
O2 -*-production was preceded by an increased influx of
lactate or
pyruvate into mitochondria and resulted in the down - regulation of the anti - apoptotic protein
bcl - X ( L ) . -
pubmed
Moreover , when the
lactate / pyruvate transporter was inhibited by
5 - nitro-2 -( 3 - phenylpropylamino ) benzoate ,
ALA- and
DHLA - induced mitochondrial ROS - production and apoptosis were blocked . -
pubmed
Variations in serum parameters like
osteocalcin , c - telopeptide , levels of elements like
Calcium ,
Magnesium ,
Phosphorus , concentration of
vitamin - D metabolites ,
lactate levels ,
bicarbonate concentrations , amount of
alkaline phosphatase are demonstrated in the course of development of
osteoporosis . -
pubmed
Mixed lysate assays demonstrate that this is the result of endogenous
inhibitors present in early embryos , suggesting a model of matrix remodeling regulated by spatially heterogeneous
MMP inhibition . -
pubmed
Isomerization of
L - aspartate and deamidation of
L - asparagine in
proteins or peptides dominantly give rise to
L - isoaspartate by a non - enzymatic reaction via
succinimide as a intermediate under physiological conditions . -
pubmed
We described here that the formation of
isoaspartate is enhanced in amyloid - beta (
Abeta ) peptides in
Alzheimer ' s disease (
AD ) . -
pubmed
Specific antibodies recognizing
isoaspartate of
Abeta revealed that isomerized
Abeta peptides were deposited in
senile plaques as well as amyloid - bearing vessels . -
pubmed
Furthermore , systematic
proline substitution analyses revealed that the beta - turn structure at positions 22 and 23 of
Abeta42 plays a crucial role in the aggregation and
neurotoxicity of
Abeta peptides . -
pubmed
Protein L - isoaspartyl methyltransferase (
PIMT ) is a putative protein repair enzyme , which converts
L - isoaspartyl residues in damaged proteins to normal
L - aspartyl residues . -
pubmed
The one -
pot conversion of
( + /-)- citronellal to
menthol can be selectively catalysed by either a bifunctional
Ni /
Zr - zeolite beta catalyst or a dual catalyst system of Zr - beta and
Ni /
MCM-41 , giving a high diastereoselectivity to
( + /-)- menthol of 90 - 94 % . -
pubmed
We introduced
methionines one - at - a - time into the region 163 - 176 of the
parathyroid hormone receptor , and find that crosslinking occurs to the side - chain of
methionine over a range of 11
amino acids . -
pubmed
The
enzyme had a K(m ) of 190 micromolar for its substrate ,
12 - oxo - phytodienoic acid . -
pubmed
The preferred reductant was
NADPH , for which the
enzyme exhibited a K(m ) of 13 micromolar , compared with 4.2 millimolar for
NADH . -
pubmed
Recently , postsource decay ( PSD ) fragment spectra obtained by matrix - assisted laser desorption - ionization time - of - flight mass spectrometry ( MALDI -
TOF MS ) have provided critical information on complex
carbohydrates . -
pubmed
On the basis of the MALDI -
PSD spectra , we deduced fragmentation rules for
phosphorylated N - glycans that will be valuable for distinguishing the position of phosphorylation . -
pubmed
In the present study , this first - shot phenomenon has been investigated for the hexameric protein complex
allophycocyanine (
APC ) by two independent methods , MALDI -
MS with a ( nearly ) pH - neutral matrix
6 - aza-2 - thiothymine (
6 - ATT ) and by imaging the fluorescence of the complex in
APC-6 - ATT preparations by confocal laser scan microscopy ( CLSM ) . -
pubmed
STUDY OBJECTIVE : To test the hypothesis that
dexmedetomidine , a selective alpha-2 agonist , enhances urine flow rate and perioperative renal function , a
post hoc analysis was conducted on a recently completed study of
dexmedetomidine used as an adjunct to epidural analgesia after thoracotomy . -
pubmed
Melatonin increases stress fibers and focal adhesions in MDCK cells : participation of
Rho - associated kinase and
protein kinase C. -
pubmed
An optimal increase in water transport , concomitant with elevated stress fiber (
SF ) formation , occurs at nocturnal plasma
melatonin concentrations ( 1 nm ) after 6 hr of incubation . -
pubmed
Blockage in
melatonin - elicited dome formation was observed with
protein kinase C (
PKC ) inhibitors . -
pubmed
Despite , this information on the precise mechanism by which
melatonin increases
SF formation involved in water transport is not known . -
pubmed
SF organization and
vinculin phosphorylation are involved in FAC assembly and both processes are mediated by
PKC , an enzyme stimulated by
melatonin ; in these processes also involved is
Rho - associated kinase (
ROCK ) . -
pubmed
Thus , we studied FAC formation and the
ROCK /
PKC pathway as the mechanism by which
melatonin increases
SF formation and water transport . -
pubmed
The results showed that 1 nM
melatonin and the
PKC agonist
phorbol-12 - miristate-13 - acetate increased
FAC . -
pubmed
The
PKC inhibitor
GF109203x , and the
ROCK inhibitor
Y27632 , blocked increased
FAC caused by
melatonin . -
pubmed
ROCK and
PKC activities ,
vinculin phosphorylation and FAC formation were increased with
melatonin . -
pubmed
The
PKC inhibitor ,
GF109203x , abolished both
melatonin stimulated
FAC in whole cells and
ROCK activity , indicating that
ROCK is a downstream
kinase in the
melatonin - stimulated
PKC pathway in MDCK cultured cells that causes an increase in
SF and FAC formation . -
pubmed
The intercalative interaction of the
Cu ( II ) complexes with calf - thymus DNA (
CT - DNA ) was probed by UV / VIS and fluorescence titration , as well as by thermal - denaturation experiments , and the intrinsic binding constants ( K(b ) ) for the complexes with 1 and 2 were 4.2x10(3 ) and 4.9x10(3 ) M(-1 ) , resp . -
pubmed
Hemoglobin vesicle ( HbV ) , which is also called liposome - encapsulated
hemoglobin , functions as a
hemoglobin - based
oxygen carrier and is expected to be utilized in emergency situations including
hemorrhagic shock and several kinds of
ischemic diseases . -
pubmed
Endovascular therapy was performed with
coils in seven cases ,
n - butyl-2 - cyanoacrylate (
NBCA ) in two cases and with both in one case . -
pubmed
We did not notice change in the levels of
creatinine phosphokinase , serum
creatinine or lover enzymes . -
pubmed
Role of
ceramide in membrane protein organization investigated by combined
AFM and FCS . -
pubmed
Our results show that components with poor affinity toward the liquid - ordered phase , such as several fluorescent lipid analogues or the synaptic protein
Synaptobrevin 2 , are excluded from
ceramide - rich domains . -
pubmed
Conversely , we show for the first time that the raft - associated protein
placental alkaline phosphatase (
GPI - PLAP ) and the
ganglioside GM1 are enriched in such domains , while exhibiting a strong decrease in lateral diffusion . -
pubmed
Labeled target DNA was prepared by
polymerase chain reaction amplification of
16S rRNA genes using a
Cy5 - labeled universal bacterial forward primer and a universal reverse primer . -
pubmed
RESULTS : Physical activity , BMI ,
high - density lipoprotein cholesterol , smoking ,
depression , and
hypertension were directly associated with
CRP and
IL-6 and inversely associated with one or more HRV variables . -
pubmed
The
3 -( 4 - cyano-3 - trifluoromethylphenyl )- 5,5 - dimethylthiohydantoin derivatives which have
carboxy - terminal side chains were synthesized and their agonistic / antagonistic activities against
androgen receptor (
AR ) measured . -
pubmed
Three ionic liquid ( IL)-based aggregates ,
1 - hexadecyl-3 - methylimidazolium bromide (
HDMIm - Br ) ,
1 - hexadecyl-3 - butylimidazolium bromide (
HDBIm - Br ) , and
1,3 - didodecylimidazolium bromide (
DDDDIm - Br ) have been applied to the development of a quantitative solid - phase microextraction ( SPME)-gas chromatography ( GC)-mass spectrometry (
MS ) method . -
pubmed
A sensitivity factor (
SF ) is defined and introduced for the first time to quantitatively compare the efficiency of the IL - based aggregates by SPME -
GC -
MS and to evaluate the partitioning strength of several
polycyclic aromatic hydrocarbons (
PAHs ) to the three IL - aggregates . -
pubmed
The ILs
HDBIm - Br and
HDMIm - Br have been used successfully to extract seven
PAHs from the certified reference sediment BCR-535 using focused microwave - assisted extraction followed by SPME -
GC -
MS . -
pubmed
When the association of
cyclin B1 with positive
MIB1 is also considered , our results support the role of cell proliferation in
FDG uptake by the
tumour . -
pubmed
Oleanolic acid is a selective
farnesoid X receptor modulator . -
pubmed
Bile acid biosynthesis and catabolism are in part controlled in the liver by
transcription factor farnesoid X receptor (
FXR ) . -
pubmed
OA also dose - dependently suppressed the activity of
FXR - LBD induced by its endogenous ligand
chenodoxycholic acid (
CDCA ) . -
pubmed
Consistently , OA partially blocked the ability of
CDCA to induce a
FXR target gene
bile salt export protein (
BSEP ) . -
pubmed
An enzymatic method to distinguish
tetrahydrobiopterin from
oxidized biopterins using
UDP - glucose : tetrahydrobiopterin glucosyltransferase . -
pubmed
We developed an enzymatic method to distinguish
BH4 from the oxidized forms by employing
BH4 : UDP - glucose alpha - glucosyltransferase (
BGluT ) , which catalyzes
glucosyl transfer from
UDP - glucose to
BH4 . -
pubmed
The
BGluT - catalyzed reaction not only would reduce the burden of chromatographic separation but also would promise non - HPLC analysis of
BH4 . -
pubmed
RESULTS : Serum free T4 and T3 levels , adjusted for
levothyroxine dose per kilogram
body weight , decreased by 11 and 18 % , respectively , whereas
TSH levels increased . -
pubmed
Studies have shown that cytosolic
GS isoforms are involved in
nitrogen remobilization during leaf senescence and emphasized a role in seed production particularly in small grain crop species . -
pubmed
It is expected that understanding the regulation of
GS under varied climatic conditions will emerge as an important new area considering the results from recent studies that have shown
nitrogen assimilation to be critical to plant acclimation to high
CO ( 2 ) concentrations . -
pubmed
An
EPR study on powdered samples confirms the presence of
Mn ( II ) ,
Fe ( III ) and
Cr ( III ) in the minerals . -
pubmed
Role of
STIM1 /
Orai1 - mediated store - operated
Ca2 + entry in airway smooth muscle cell proliferation . -
pubmed
Store - operated
Ca ( 2 + ) ( SOC ) entry ( SOCE ) occurs in response to the intracellular sarcoplasma reticulum ( SR)/endoplasmic reticulum (
ER )
Ca ( 2 + ) store depletion . -
pubmed
Stromal interaction molecule ( STIM ) 1 has been proposed as an
ER /
SR Ca ( 2 + ) sensor and translocates to the
ER underneath the plasma membrane upon depletion of the
ER Ca ( 2 + ) store , where it interacts with
Orai1 , the molecular component of SOC channels , and brings about SOCE . -
pubmed
We found that
SOCE was upregulated during ASMC proliferation accompanied by a mild increase of
STIM1 and a significant increase of
Orai1 mRNA expression , whereas the proliferation of ASMCs was partially inhibited by the SOC channel blockers
SKF-96365 ,
NiCl ( 2 ) , and
BTP-2 . -
pubmed
Moreover , after knockdown of
STIM1 or
Orai1 , the SOC channel blocker
SKF-96365 had no inhibitory effect on the proliferation of ASMCs anymore . -
pubmed
The existence of a stable pi - electron complex ( called
A1 ) between a
tert - butyl cation and a
benzene molecule has been confirmed . -
pubmed
XPG is a structure - specific
endonuclease required for
nucleotide excision repair (
NER ) . -
pubmed
The parameters for comparison included
body mass index (
BMI ) , the distance from the renal hilum to scrotum (
DRS ) , semen quality and pH value , serum concentration of
follicle - stimulating hormone (
FSH ) ,
luteinizing hormone (
LH ) ,
prolactin ,
testosterone ,
alkaline phosphatase (
Alk - p ) ,
lactate dehydrogenase (
LDH ) , testicular volume and discrepancy ( % ) , grade of
varicocoele and peak retrograde flow ( PRF ) and
spontaneous venous reflux ( SVR ) by colour Doppler ultrasound and scrotal temperature ( ST ) . -
pubmed
A2 is an anaerobic gram - negative
sulfate - reducing bacterium with remarkable tolerance to
copper . -
pubmed
The DB extract effectively reversed the HFD - induced elevations in plasma
glucose and
insulin levels , and the homeostasis model assessment for
insulin resistance and oral
glucose tolerance test values . -
pubmed
These studies indicate that
MLN0905 modulates the pharmacodynamic biomarker phosphorylated
histone H3 (
pHisH3 ) in
tumor tissue . -
pubmed
If ovulation can not be induced by
CC , then either laparoscopic ovarian drilling ( LOD ) or
gonadotropin therapy is selected as a subsequent treatment . -
pubmed
IUI with administration of
CC plus recombinant
follicle - stimulating hormone (
rFSH ) was carried out ; however , pregnancy was not achieved . -
pubmed
In the first attempt , the development of several follicles was observed under the
gonadotropin releasing hormone (
GnRH ) agonist long protocol . -
pubmed
Similarly ,
CAB - E4 was also found to decrease the production of EPS ( 43 - 57 % ) ,
hemolysin ( 43 - 57 % ) and biofilms ( 80 - 85 % ) of test pathogens . -
pubmed
A drug for the treatment of
sexual dysfunction ,
sildenafil , enhances the electrically evoked release of
OT from the posterior pituitary . -
pubmed
Copeptin as a marker of relative
arginine vasopressin deficiency after pediatric cardiac surgery . -
pubmed
The breakdown of
L - arginine to
ornithine and
urea by host
arginase supports Leishmania proliferation in macrophages . -
pubmed
OBJECTIVE : To evaluate the difference in time until medical clearance when comparing tincture of opium ( TO ) to oral
morphine (
OM ) in the treatment of
neonatal abstinence syndrome (
NAS ) . -
pubmed
MAIN OUTCOME MEASURE(S ): The primary outcome is to compare the time it takes for a baby being treated with
TO versus
OM for NAS to be medically cleared for discharge . -
pubmed
RESULTS : The median time until medical clearance for those treated with
TO was 29.5 days compared to 37 days for those treated with
OM ( p = 0.14 ) . -
pubmed
CONCLUSION : There was no statistically significant difference in the time it takes for a baby being treated with
TO versus
OM for NAS to be medically cleared for discharge . -
pubmed
There are a number of safety benefits in using
OM compared to
TO . -
pubmed
PURPOSE : The purpose of this study was to investigate the possible association between single
nucleotide polymorphisms ( SNPs ) rs1800629 (
TNF - alpha -308 ) and rs361525 (
TNF - alpha -238 ) of the
tumour necrosis factor ( TNF)-alpha gene and susceptibility to
osteoarthritis (
OA ) in the Han Chinese population . -
pubmed
After the separation of
proteins under these first metal - free conditions , the metal ions in the gel fractions are eluted , followed by derivatization of
copper ions into the metal probe complexes to be separated and determined by fluorescence detection in the second PAGE . -
pubmed
In this PAGE - based method , the
copper ions bound to
ceruloplasmin and
superoxide dismutase were quantitatively determined , in addition to the exchangeable
albumin - bound
copper ions . -
pubmed
INTERVENTIONS : ISI was estimated from serial measurements of plasma
insulin and
glucose during an iv
glucose tolerance test . -
pubmed
Polo - like kinase 2 (
PLK2 ) was previously proposed as an important
kinase in
alpha - synuclein phosphorylation at
Ser129 . -
pubmed
Whereas
PLK2 knockdown had no effect on Total -
alpha - synuclein brain levels , it resulted in a gene - dosage dependent , albeit incomplete , reduction of endogenous
Phospho - alpha - Syn levels in all brain regions investigated . -
pubmed
These results support a significant role for a
PLK kinase in phosphorylating
alpha - synuclein at
Ser129 in the brain , and suggest that
PLK2 is responsible for this activity under physiological conditions . -
pubmed
Dutasteride treatment contributed to a significant increase in
BMD in group A. -
pubmed
Asp(112 ) , in the middle of the
S1 transmembrane helix , is an essential part of the selectivity filter in wild - type ( WT ) channels . -
pubmed
After neutralizing
Asp(112 ) by mutating it to
Ala ( D112A ) , we introduced
Asp at each position along
S1 from 108 to 118 , searching for " second site suppressor " activity . -
pubmed
The
S4 segment of this mutant has similar accessibility to WT in open channels , because R211H / D112V / V116D was inhibited by internally applied
Zn ( 2 + ) . -
pubmed
At both positions where it produces proton selectivity ,
Asp frequently engages in salt linkage with one or more
Arg residues from
S4 . -
pubmed
The type 4 P - type
ATPases are phospholipid flippases that generate such membrane
phospholipid asymmetry . -
pubmed
Here we show that the
carboxyl - terminal cytoplasmic region of
Drs2p directly binds to
Rcy1p , an F - box protein that is also required for endocytic recycling . -
pubmed
The
Drs2p - binding region was mapped to the
amino acids 574 - 778 region of
Rcy1p and a mutant
Rcy1p lacking this region was defective in endocytic recycling of a
v - SNARE Snc1p . -
pubmed
In this work , we propose a novel
glucose binding mechanism on a highly sensitive
SERS substrate , in order to overcome challenges in specific
glucose detection in bio - fluids . -
pubmed
Chloroethene degradation and expression of
Dehalococcoides dehalogenase genes in cultures originating from Yangtze sediments . -
pubmed
Computational & amp ; experimental evaluation of the structure / activity relationship of
beta - carbolines as
DYRK1A inhibitors . -
pubmed
AOM was first found to be coupled with
sulfate reduction and mediated by anaerobic methanotrophic archaea ( ANME ) and
sulfate - reducing bacteria ( SRB ) . -
pubmed
Moreover ,
manganese (
Mn ( 4 + ) ) and
iron (
Fe ( 3 + ) ) can also be used as electron acceptors of
AOM . -
pubmed
Analogous to a eukaryotic cap , 5'-
NAD modification is shown in vitro to stabilize RNA against 5'-processing by the
RNA - pyrophosphohydrolase RppH and against endonucleolytic cleavage by
ribonuclease ( RNase ) E. -
pubmed
The
nudix phosphohydrolase NudC decaps
NAD - RNA and thereby triggers
RNase - E - mediated RNA decay , while being inactive against
triphosphate - RNA . -
pubmed
In vivo , ~13 % of the abundant sRNA RNAI is
NAD - capped in the presence , and ~26 % in the absence , of functional
NudC. -
pubmed
Ethanol and
corticotropin releasing factor receptor modulation of central amygdala neurocircuitry : An update and future directions . -
pubmed
Much of the work examining the mechanisms by which the central amygdala mediates the development of
alcohol dependence has focused on the interaction of acute and chronic
ethanol with central amygdala
corticotropin releasing factor signaling . -
pubmed
Much of this work has primarily focused on the hypothesis that
ethanol utilizes endogenous
corticotropin releasing factor signaling to upregulate inhibitory GABAergic transmission in the central amygdala . -
pubmed
Work that is more recent suggests that
corticotropin releasing factor also plays an important role in mediating
anxiety - like behaviors via the enhancement of central amygdala glutamatergic transmission , implying that
ethanol /
corticotropin releasing factor interactions may modulate excitatory neurotransmission in this brain region . -
pubmed
The micelle would be successful in increasing drug solubility , however it rather decreased permeability of model drug
progesterone ( Biopharmaceutics Classfication System (
BCS ) Class
II ) as an overall flux . -
pubmed
Thirdly , in the case of injection of
paclitaxel (
BCS Class II)formulation , the drug was adsorbed to
albumin . -
pubmed
However , it was observed that some possible
n - butanol tolerance mechanisms did not occurred upon the
ATF expression , especially the membrane - related functions such as the homeoviscous adaptation ,
iron uptaking system , and efflux pump system . -
pubmed
The purpose of this study was to establish a population pharmacokinetic ( PK ) model of
tacrolimus and evaluate the influence of clinical covariates , including the genetic polymorphisms of the
cytochrome P450 3A5 gene (
CYP3A5 ) and gene - encoding
P - glycoprotein (
ABCB1 ) , on the
PK parameters in Chinese adult liver transplant recipients . -
pubmed
In the final model ,
CYP3A5 genotype ,
post - operative day ,
alanine aminotransferase , total
bilirubin , hematocrit and blood
urea nitrogen were found to significantly influence the CL / F , whereas POD and HB influence V / F. 4 . -
pubmed
Population PK analysis of
tacrolimus in Chinese adult liver transplant patients resulted in identification of the
CYP3A5 genotype , POD ,
BUN ,
ALP , HCT , TBIL and HB as significant covariates on the PK parameters of
tacrolimus . -
pubmed
MATERIALS AND METHODS : The number of primary AECIIs , their viability , and cell cycle and apoptosis were studied under
KGF treatment using a hemocytometer ,
trypan blue exclusion , and flow cytometry , respectively . -
pubmed
The advantage of this design is that the targeted knockin retains the intact endogenous
aggrecan locus and expresses the
tamoxifen - inducible CreERT2 protein from a second IRES - driven open reading frame . -
pubmed
Hereditary 1 , 25 - dihydroxyvitamin D - resistant rickets (
HVDRR ) , a rare
recessive disease , is caused by mutation in the
VDR gene encoding the
vitamin D receptor leading to the resistance to
vitamin D. -
pubmed
Insulin Resistance in Nondiabetic Peritoneal Dialysis Patients : Associations with Body Composition , Peritoneal Transport , and Peritoneal
Glucose Absorption . -
pubmed
Leptin ,
adiponectin ,
insulin - like growth factor - binding protein 1 (
IGFBP-1 ) , and daily
glucose absorption were also measured . -
pubmed
FM ( 7.3 [ 12.3 , interquartile range ] versus 0.7 [ 1.4 , interquartile range ] , P<0.001 , and 39.4 + - 10.1 % versus 27.2 + - 11.5 % , P=0.002 , respectively ) , lower
IGFBP-1 ( 8.2 + - 7.2 versus 21.0 + - 16.3 ng / ml , P=0.002 ) , but similar
glucose absorption and small - solute transport compared with patients without
insulin resistance . -
pubmed
Urinary and serum levels of
NGAL have been investigated as a new biomarker of
acute kidney injury (
AKI ) , for an earlier and more accurate detection method than with
creatinine level . -
pubmed
Moreover , we purified the
NGAL with
Ni - Sepharose column . -
pubmed
The dynamics and the vibrational signatures are used to interpret IR -
MPD spectra recorded in the
NH /
OH stretch region . -
pubmed
Indole based
tubulin polymerization inhibitors : An update on recent developments . -
pubmed
Diverse classes of naturally occurring as well as synthetic and semi - synthetic compounds with an
indole nucleus induce microtubule polymerization and depolymerization and thereby change
tubulin dynamics . -
pubmed
The report mainly focuses on the brief synthetic methodology with the relevant
SAR studies of different
indole derived molecules that have been reported in the past few years as potential inhibitors of
tubulin polymerization is discussed . -
pubmed
The cellular and molecular effects of
oxysterols were then evaluated on primary cultures of ASCs focusing on cell viability , adipogenic differentiation , and " canonical " WNT and
MAPK signaling pathways . -
pubmed
Notably , the accumulation of
oxysterols in adipocytes was predicted by the adipose cell size of the donor (
R2 = 0.582 ; P & lt ; .01 ) . -
pubmed
Challenging ASCs with free radical - derived type I ( 7kappa - C ) and type
II (
5,6 - Secosterol ) oxysterols led to a time- and concentration - dependent decrease of cell viability . -
pubmed
We found a statistically significant increase ( nominal p & lt ; 0.05 ) in the expression of
UGT1A1 ,
UGT1A3 ,
UGT1A4 ,
UGT1A5 ,
UGT1A6 ,
UGT2B7 , and
UGT2B17 , as well as glucuronidation activities of
serotonin ,
testosterone , and
vorinostat during the first 25 years of life . -
pubmed
Greater
RPE was reported in
4vs4 - NO ( p & lt ; 0.01 ) and 4vs4 + 2IW - NO ( p & lt ; 0.01 ) compared with 4vs4 + 2IW+2IEW -
NO , and during 4vs4 -
GK ( p & lt ; 0.01 ) than 4vs4 + 2IW+2IEW -
GK . -
pubmed
Pterostilbene attenuates high
glucose - induced oxidative
injury in hippocampal neuronal cells by activating
nuclear factor erythroid 2 - related factor 2 . -
pubmed
High
glucose medium ( 100mM ) decreased cellular viability after 24h incubation which was evidenced by : ( i ) reduced mitochondrial
complex I and III activities ; ( ii ) reduced mitochondrial
cytochrome C ; ( iii ) increased reactive
oxygen species ( ROS ) generation ; ( iv ) decreased mitochondrial membrane potential ( DeltaPsim ) ; and ( v ) increased
lactate dehydrogenase (
LDH ) levels . -
pubmed
PTE ( 2.5 , 5 , and 10muM for 24h ) was nontoxic and induced the nuclear transition of
Nrf2 . -
pubmed
Pretreatment of
PTE ( 2.5 , 5 , and 10muM for 2h ) displayed a dose - dependently neuroprotective effect , as indicated by significantly prevented high
glucose - induced loss of cellular viability , generation of ROS , reduced mitochondrial
complex I and III activities , reduced mitochondrial
cytochrome C , decreased DeltaPsim , and increased
LDH levels . -
pubmed
In addition , the elevation of nuclear
Nrf2 by
PTE treatment ( 10muM for 2h ) was abolished by
Nrf2 siRNA . -
pubmed
Overall , the present findings were the first to show that
pterostilbene attenuated high
glucose - induced
central nervous system injury in vitro through the activation of
Nrf2 signaling , displaying protective effects against
mitochondrial dysfunction - derived oxidative stress . -
pubmed
The plant - specific
PIN - FORMED (
PIN ) auxin efflux proteins have been well characterized in many plant species , where they are crucial in the regulation of
auxin transport in various aspects of plant development . -
pubmed
NtPIN4 expression was strongly induced by the application of exogenous
indole-3 - acetic acid (
IAA ) , but was downregulated by the application of
ABA , a
strigolactone analogue , and
cytokinin , as well as by
decapitation treatments , suggesting that the
NtPIN4 expression level is likely positively regulated by auxin . -
pubmed
Therefore ,
NtPIN4 offers an opportunity for studying
auxin - dependent branching processes . -
pubmed
Resveratrol protects
bupivacaine - induced neuro - apoptosis in dorsal root
ganglion neurons via activation on
tropomyosin receptor kinase A. -
pubmed
QRT - PCR and western blot assays were also conducted to evaluate gene and protein expressions of
tropomyosin receptor kinase A / B / C (
TrkA / B / C ) and activated ( phosphorylated )
Trk receptors ,
phospho -
TrkA / B / C. -
pubmed
qRT - PCR and western blot showed that
RSV did not alter
TrkA / B / C gene or protein expression , but significantly upregulated
phospho -
TrkA. -
pubmed
CONCLUSION :
Resveratrol may protect anesthesia - induced DRG neuro - apoptosis , and activation of
TrkA signaling pathway may be the underlying mechanism in this process . -
pubmed
Acrylamide and
N , N ' - methylene bisacrylamide was used as carrier materials and the N - terminal epitope
ofnicotinic acetylcholine receptor alpha7 (
nAchR alpha7 ) was tested as a template molecule , and the polymer nanoparticleswere obtained after polymerization and template removal . -
pubmed
Specifically ,
P7C3 promoted the expression of neurogenesis - associated proteins , including
doublecortin ,
beta tubulin III (
beta - tub3 ) ,
adam11 and
adamts20 , near the peri -
infarct cortex , accompanied by
glycogen synthase kinase 3 (
GSK-3 ) inhibition and
beta - catenin upregulation . -
pubmed
The application of a specific inhibitor against
glucagon - like peptide 1 receptor (
GLP-1R ) ,
exendin ( 9 - 39 ) , revealed that the beneficial effects of
P7C3 involved triggering the activation of
GLP-1R - associated
PKA /
Akt signaling . -
pubmed
P7C3 elicited the
GLP-1R - dependent intracellular
cAMP increment and the
insulin secretion in cellular models . -
pubmed
Our findings indicate that
P7C3 promotes the expression of neurogenesis proteins by activation of the
cAMP /
PKA - dependent and
Akt /
GSK3 - associated
beta - catenin through positive allosteric stimulation of
GLP-1R. -
pubmed
UV - vis of
Cu - NPLs showed an
SPR characteristic peak at 563 nm ( energy bandgap = 2.1 eV ) . -
pubmed
Morphology and size of the as - prepared
Cu - NPLs were determined using SEM and
TEM studies . -
pubmed
TEM observations show that the produced
Cu - NPLs are hemispherical in shape with different diameters in the range 4.7 - 17.4 nm . -
pubmed
Cell proliferation in
FXR1 - deficient cells was evaluated by cell counting and
MTT assays . -
pubmed
The
ESR1 open reading frame ( ORF ) was 1 , 767 bp encoding 588
amino acids . -
pubmed
For
BMPR1B , the ORF was 1599 bp encoding 532
amino acids , and an ORF of 906 bp encoding 301
amino acids was identified in
FOXL2 . -
pubmed
Methods Nine
fentanyl analogues (
2 , 2 '- difluorofentanyl ,
acetyl fentanyl ,
fentanyl ,
butyl fentanyl ,
valeryl fentanyl ,
acryloyl fentanyl ,
furan fentanyl ,
4 - fluorine isobutyl fentanyl ,
carfentanyl ) were selected and analyzed with ultra - high performance liquid chromatography - quadrupole time - of - flight - mass spectrometry ( UHPLC - QTOF -
MS ) and gas chromatography - mass spectrometry (
GC -
MS ) . -
pubmed
Hence , we attempted to carry out in silico screening of
benzene sulfonamide substituted
pyrazole - pyrazoline series against
Histo aspartic protease . -
pubmed
Molecular dynamic simulation study of free
enzyme and the
enzyme complex with
4 -( 5 -( 4 - methoxyphenyl )- 1'phenyl-3 '-( p - tolyl )- 3,4 - 1'H,2H -[ 3,4 '- bipyrazol ]- 2 - yl ) benezenesulfonamide indicated structural stability . -
pubmed
Tandem RNA isolation reveals functional rearrangement of RNA - binding proteins on
CDKN1B /
p27Kip1 3'UTRs in
cisplatin treated cells . -
pubmed
Here we introduce a novel biochemical approach , termed
tobramycin - based tandem RNA isolation procedure ( tobTRIP ) , to quantify proteins associated with the 3'UTRs of
cyclin - dependent kinase inhibitor 1B (
CDKN1B /
p27Kip1 ) mRNAs in vivo . -
pubmed
P27Kip1 plays an important role in mediating a cell 's response to
cisplatin (
CP ) , a widely used chemotherapeutic
cancer drug that induces DNA damage and cell cycle arrest . -
pubmed
RESULTS : A high expression of
PRDX-1 was observed in 20 % of cases , and was associated with worse compliance to chemotherapy protocol ( P<0.002 ) , worse response to chemotherapy ( P<0.04 ) , and higher levels of
CA 125 after the
1 < sup > st sup > line treatment ( P<0.004 ) . -
pubmed
This study explores the simultaneous application of fly ash (
FA ) generated from the thermal treatment of municipal solid waste as a
CO2 sequester through aqueous mineral carbonation and as a supplementary cementitious material ( SCM ) for the development of green construction materials . -
pubmed
Stratifying analyses by the levels of measurement of
LAN exposures ( macro- , meso- and micro - levels ) and time of
LAN measurement ( including before and while sleeping ) consistently produced robust estimates , with higher exposure to
LAN being positively associated with poorer weight outcomes . -
pubmed
Luteolin affects
keloid fibroblast proliferation and apoptosis by regulating
FRAT1 gene expression . -
pubmed
The current experiment was performed to investigate whether
luteolin affects the proliferation and apoptosis of
keloid fibroblasts by regulating the expression of
FRAT1 gene . -
pubmed
MTT method , western blot , flow cytometry , and real - time quantitative PCR ( qPCR ) were used to detect cell proliferation ,
cyclin D1 (
CyclinD1 ) ,
p21 ,
B - cell lymphoma / leukemia-2 (
Bcl-2 ) ,
Bcl-2 related X protein (
Bax ) ,
FRAT1 protein expression , apoptosis and
ARHI mRNA expression . -
pubmed
Keloid fibroblasts were transfected with si -
FRAT1 , or pcDNA -
FRAT1 and treated with
luteolin to observe their roles in cell proliferation and apoptosis . -
pubmed
Compared with the control group ,
luteolin significantly reduced the
keloid fibroblast activity ,
CyclinD1 ,
Bcl-2 , and
FRAT1 protein levels , and obviously improved the cell apoptosis rate ,
p21 and
Bax protein expression ( P<0.05 ) . -
pubmed
FRAT1 overexpression reversed the inhibitory effect of
luteolin on
keloid fibroblast activity ,
FRAT1 ,
CyclinD1 , and
Bcl-2 protein expression , and promotion of apoptosis ,
p21 and
Bax protein expression . -
pubmed
Luteolin can inhibit the proliferation and induce apoptosis of
keloid fibroblasts by regulating the expression of
FRAT1 gene . -
pubmed
: Bar charts ( top ) show the percentage of
BrdU ( a ) and
Ki67 ( b ) positive cells relative to the percentage of
DAPI stained nuclei ( y - axis ) in cells at baseline and serially passaged cells ( x - axis ) . -
pubmed
: The bar charts ( right ) show the percentage of
MAP-2 ( a ) ,
CC3 ( b ) ,
S100beta ( c ) and
doublecortin (
DCX ) ( d ) -positive cells relative to the percentage of
DAPI stained nuclei in cells at baseline and serially passaged cells . -
pubmed
The mean value of diastolic and
systolic blood pressures ,
pulse rate , and mean arterial pressure
dioxide were significantly decreased in both PF and
PP groups ( P Time < 0.001 ) , although there was no significant difference between groups . -
pubmed
Cynaropicrin Shows Antitumor Progression Potential in
Colorectal Cancer Through Mediation of the
LIFR /
STATs Axis . -
pubmed
Western blotting results suggested that the antitumor effects of
cynaropicrin might be mediated by inhibition of the
LIFR /
STATs axis . -
pubmed
Cynaropicrin reduced the formation of
STAT3 /
STAT4 heterodimers and blocked their entry into the nucleus . -
pubmed
Exposure to
NPS ( 1 - 100 mug / L ) increased the
glb-10 expression , and the
glb-10(RNAi ) worm was susceptible to
NPS toxicity in inducing reactive
oxygen species ( ROS ) production and in decreasing locomotion behavior . -
pubmed
Moreover , a signaling cascade of
HRG-7 -
HRG-5 required for the control of
heme homeostasis was identified to function downstream of neuronal
GLB-10 to regulate the
NPS toxicity . -
pubmed
Our results highlight the crucial function of
heme homeostasis related signaling in regulating the
NPS toxicity in organisms . -
pubmed
The accumulation of
dopa and
5 - hydroxytryptophan after inhibition of the
aromatic amino acid decarboxylase with
3 - hydroxybenzylhyrazine HCl , served as a measure of
catecholamine and
5 - hydroxytryptamine synthesis in vivo . -
pubmed
The data are compatible with the view that
dibutyryl cAMP stimulates
tyrosine and
tryptophan hydroxylase directly . -
pubmed
The reaction of
tris ( hydroxymethyl ) aminomethane with
calf intestinal alkaline phosphatase . -
pubmed
The effect of
tris ( hydroxymethyl ) aminomethane concentrations on the rate of calf intestinal
alkaline phosphatase - catalyzed hydrolysis of
p - nitrophenyl phosphate was studied , in the pH range 8 - 10 , where no transphosphorylation reaction could be detected . -
pubmed
Kinetic analysis of the results permitted description of the effect of
Tris concentrations T on the rate of
enzyme catalyzed hydrolysis ( V ) by the following equation : ( see article ) . -
pubmed
The rate - accelerating effect of
Tris concentrations can be ascribed to two different mechanisms : At moderate
Tris concentrations ( 0.01 - 0.20 M ) the
enzyme forms a reversible addition complex with a
Tris molecule . -
pubmed
We suggest that the binding of
Tris to the
enzyme could potentiate a second active site of the
enzyme , due to its ionization effect upon an acidic group of the
enzyme of pK = 8.9 . -
pubmed
The modest linear rate accelerating effect of
Tris at high concentrations ( 0.20 - 0.60 M ) could be ascribed to the change of the dielectric constant of the medium , the degree of solvation of the
protein , or change in the tertiary structure of the
enzyme . -
pubmed
The mechanism of antisecretory activity currently remains unknown as
UP 5145 - 52 fails to bind to receptors involved in gastric secretion (
H2 or muscarinic ) and to inhibit
H +/ K + adenosine triphosphatase . -
pubmed
The reaction required an
NADPH - generating system and molecular
oxygen and was inhibited by
carbon monoxide , suggesting that a
cytochrome P450 - linked mono - oxygenase system is prerequisite for the deactivation reaction . -
pubmed
Intraperitoneal
IL-2 and
LAK cells led to elevations in the concentrations of
SP and
CGRP to respective maximal means of 319 and 175 pM after 8 hr , which were maintained for 24 - 48 hr without alterations in the levels of
vasoactive intestinal peptide or
somatostatin . -
pubmed
Circadian rhythm of
atrial natriuretic peptide ,
plasmatic renin activity , and plasma
aldosterone in compensated
cirrhosis of the liver . -
pubmed
The activities of
rat brain protein kinase C isoenzymic fractions separated by
hydroxyapatite chromatography were measured with
histone H1 or the oligopeptide
Ala - Ala - Ala - Ser - Phe - Lys - Ala - Lys - Lys - amide as substrates . -
pubmed
In some brain extracts a phospholipid - dependent but
Ca2 + -inhibited
protein kinase was also observed which was eluted from the
hydroxyapatite column between type
II and III isoenzymes of
protein kinase C. -
pubmed
Amino acids and peptides XII : synthetic peptides related to the N - terminal portion of
fibrin alpha - chain and their inhibitory effects on
fibrinogen /
thrombin clotting . -
pubmed
Of the seven sites at which nucleotide substitutions were confirmed using RNA templates , four resulted in
amino acid changes in three different proteins , the leader peptide , 1B ( VP2 ) , and
1D (
VP1 ) . -
pubmed
SOD derivatized with
polyethylene glycol , which increases circulating half - life , was more effective , increasing survival to 80 % . -
pubmed
Desferal -
Fe3 + , which lacks in vitro
SOD activity , or
Mn2 + alone did not affect the survival of skin flaps , but
Desferal alone was nearly as effective as the
Desferal -
Mn2 + mixture . -
pubmed
The cleansing and application of a molluscicide (
copper sulphate ) to the raceways in
spring resulted in a 56 % reduction in the numbers of metacercariae infecting trout during the following summer . -
pubmed
The subunit structure has been analysed by
SDS polyacrylamide gel electrophoresis of the highly purified preparation and by immunoprecipitation with a monoclonal antibody directed to the
DNA polymerase . -
pubmed
The present results indicate that an inhibition of
beta 2 - mediated hepatic glycogenolysis by
propranolol may also influence blood
glucose homeostasis during exercise . -
pubmed
Naloxone has been used as a pharmacological tool to investigate the role of endorphins and
opiate receptors in the cardiovascular pathophysiology of
shock . -
pubmed
It would appear that endorphins act on
opiate receptors to contribute to the abnormalities found and that
naloxone improves survival as well as cardiovascular function in
shock . -
pubmed
Naloxone has served us well as a key to unlock the involvement of endorphins and
opiate receptors in
shock . -
pubmed
Naloxone 's disadvantage of increasing
pain awareness may limit its clinical usefulness but might be overcome by using
drugs that reverse the behavioral and neuroendocrine changes produced by
beta - endorphin without altering
pain relief . -
pubmed
Thyrotropin - releasing hormone (
TRH ) is just such a " physiological " opiate antagonist which has been shown to increase MAP in experimental
endotoxic and hemorrhagic shock [ 32 ] . -
pubmed
Calcium restriction and
parathyroid hormone enhance renal compensatory growth . -
pubmed
The data support the hypothesis that the inhibition of
glucagon release from the
A2 - cell is regulated via an intracellular , energy - dependent mechanism . -
pubmed
Refolding of reduced and denatured
hTSHR - ecd occurred in polyacrylamide gels treated with 0.25 M
KCl for visualization of protein bands . -
pubmed
Under conditions of partial renaturation , at least three forms of the
hTSHR -
ecd were resolved by reelectrophoresis using
sodium dodecyl sulfate - polyacrylamide gel electrophoresis : 1 ) unfolded monomers , 2 ) folded monomers , and 3 ) tetramers . -
pubmed
A natural variant of the
hTSHR ( v1.3 ) , sharing 231 N - terminal
amino acids with
hTSHR -
ecd , formed folded monomers and dimers on renaturation , but not tetramers , implicating one or more of the five
cysteine residues residing between positions 231 - 415 in the association of dimers into tetramers . -
pubmed
Na + , K (+)- ATPase ( the
sodium pump ) is a ubiquitous enzyme that consumes
ATP to maintain an adequate neuronal transmembrane electrical potential necessary for brain function and to dissipate ionic transients . -
pubmed
To assess whether abnormalities in the
sodium pump occur in this disease , we used [
3H]
ouabain to determine the density of
Na + , K (+)- ATPase for each anatomic region of hippocampus by in vitro autoradiography . -
pubmed
We estimated regional differences in capacity for
ATP generation by determining
cytochrome c oxidase ( CO ) activity . -
pubmed
Both
epilepsy groups exhibited slight but significant increases in
sodium pump density / unit mass of protein in the dentate molecular layer ,
CA2 , and subiculum as compared with autopsy controls . -
pubmed
Results suggest that although
sodium pump protein in surviving neurons appears to be upregulated in
epilepsy ,
sodium pump capacity may be limited by the reduced levels of
CO activity . -
pubmed
By far the most abundant
message was that for
amphiglycan ,
CD44 message was next in relative abundance , and the messages for
fibroglycan ,
glypican and
betaglycan were all expressed at low levels . -
pubmed
Syndecan message could not be detected by this technique . -
pubmed
In contrast with the fibroblasts , where the
amphiglycan message exhibits no size polymorphism , the chondrocyte
amphiglycan message is present in three polymorphic forms , due to the use of alternative polyadenylation signals . -
pubmed
When the newly isolated chondrocytes are maintained in monolayer culture for several passages , the
amphiglycan message heterogeneity reverts to that characteristic of the fibroblasts . -
pubmed
Dichain - TeTx , with its light chain linked to the heavy chain by means of a
disulfide bond , causes the block of exocytosis to develop more slowly than does the purified light chain . -
pubmed
The
disulfide bonds , which in both toxins hold the subunits together , were cleaved by the intrinsic
thioredoxin - reductase system . -
pubmed
Previous studies have shown that this plasmid , designated as pSMB 74 , encodes genes associated with the production of
prepediocin , its
post - translation processing to
pediocin AcH , transmembrane translocation of these molecules , and immunity of producer cells against
pediocin AcH. -
pubmed
The four genes , each with independent ribosome binding sites (
rbs ) , initiation and termination codons and spacer sequences in between , were designated as
pap A ,
pap B ,
pap C and
pap D and encode respectively for proteins of 62 , 112 , 174 and 724
amino acids . -
pubmed
Ca2 + efflux from cultured vascular smooth muscle cells ( VSMC ) consists of two mechanisms : one is dependent on extracellular
sodium , mediated by the
Na(+)-
Ca2 + exchange (
Na+o - dependent
Ca2 + efflux ) , and the other is independent of extracellular
sodium but is mediated by the
Ca ( 2 +)- pump (
Na+o - independent
Ca2 + efflux ) . -
pubmed
An increase in intracellular [
Ca2+]i may be compensated for by enhanced
Ca ( 2 +)- pump and
Na(+)-
Ca2 + exchange activities in VSMC in
hypertension . -
pubmed
These responses can also be observed after exposure to
forskolin , which activates
cyclic AMP - dependent protein kinase A or after treatment with known activators of
protein kinase C (
PKC ) such as
phorbol-12 - myristate 13 - acetate (
PMA ) . -
pubmed
The responses elicited by exposure to
8 - OH - DPAT or
PMA can be blocked completely by inhibitors of
PKC and also by 24 - hr exposure to
PMA . -
pubmed
Inhibition of
phospholipase A2 with
quinacrine or by removal of extracellular
Ca ++ blocks the agonist - mediated loss of
5 - HT1A receptor binding sites . -
pubmed
The
carbohydrates recognized by
mAbs 2B8 and 9 - OE were also abundantly expressed in the ducts and acini of the lingual salivary glands . -
pubmed
These cells were normal in other parameters and could fire throughout a depolarizing current injection in the presence of inorganic
calcium channel blockers or
norepinephrine ( NE ) , suggesting that the inability to fire was not due to
injury . -
pubmed
NE decreased the slow
Ca - dependent AHP via
beta - adrenergic receptors . -
pubmed
Serum - induced membrane depolarization in quiescent fibroblasts : activation of a
chloride conductance through the
G protein - coupled LPA receptor . -
pubmed
We find that this serum activity is attributable to
albumin - bound
lysophosphatidic acid (
LPA ) acting on its own
G protein - coupled receptor , and that membrane depolarization is due to activation of an anion conductance mediating
Cl- efflux . -
pubmed
This depolarizing
Cl- current can also be activated by
thrombin and
neuropeptide receptors ; it is distinct from volume - regulated
Cl- currents . -
pubmed
Activation of the
Cl- current consistently follows stimulation of
phospholipase C and coincides with remodelling of the
actin cytoskeleton , which is regulated by the
Ras - related GTPase
Rho . -
pubmed
The results indicate that in quiescent fibroblasts ,
LPA and other
G protein - coupled receptor agonists evoke membrane depolarization by activating a new type of
Cl - channel through a signalling pathway that is closely associated with
phosphoinositide hydrolysis , yet independent of known second messengers . -
pubmed
Mean 24 h ambulatory
SBP /
DBP decreases were 6.6 + /- 18.0/8.4 + /- 8.5 mm Hg in the
trandolapril group ( P & lt ; 0.001 ) and 5.7 + /- 11.1/7.2 + /-9.6 mm Hg in the
nitrendipine group ( P & lt ; 0.001 ) . -
pubmed
Mutation to
Phe of all four cytoplasmic
Tyr residues together (
ALL F mutant ) greatly reduced the ability of
mCD28 to augment
IL-2 production . -
pubmed
Isolated re - constitution of
Tyr 188 , but not 170 , 185 , or 197 , restored the ability of
ALL F mCD28 to deliver a costimulus . -
pubmed
The
transposon Tn10 - encoded metal - tetracycline / H + antiporter (
TetA(B ) ) was preferentially photolabeled when
[ 3H ] tetracycline was irradiated in the presence of energized inverted membrane vesicles containing the
TetA protein . -
pubmed
Photolabeling was not observed in vesicles containing the
Asp-285 --> ;
Asn mutant
TetA protein , indicating that
Asp-285 participates in the substrate binding or the step(s ) prior to substrate binding . -
pubmed
After hospitalization , urinary
C - peptide excretion (
UCP ) and 24 - hour serum profiles for
glucose were measured before and after administration of NPH
insulin 0.1 , 0.2 , and 0.3 U x kg
body weight per day subcutaneously in a single dose on 4 consecutive days . -
pubmed
To our knowledge no transcription factors have previously been shown to depend on
testosterone or
LH for expression in Sertoli cells in vivo . -
pubmed
Collectively , the data implicate
Pem as a candidate to regulate a subset of
androgen - dependent genes in the male reproductive system . -
pubmed
Concordance of
nucleotide sequences from different tissue compartments obtained concurrently from individual patients was high : 98 % in the
protease and 94 % in the
reverse transcriptase regions . -
pubmed
The prevalence of minimal or mild
endometriosis was higher in women age 25 years or older , in those who reported menarche at the age of 13 years [ prevalence odds ratio (
POR ) = 1.63 ; 95 % confidence interval ( CI ) = 1.02 - 2.60 ] or older ( POR = 1.73 ; 95 % CI = 1.07 - 2.78 ) , menstrual cycles of 27 days or less (
POR = 1.63 ; 95 % CI = 1.02 - 2.60 ) , or
caffeine intake of 300 mg per day or more ( POR = 1.33 ; 95 % CI = 0.91 - 1.94 ) . -
pubmed
In the search for an alternative to chemical halogenation reactions using the free
halogens , a novel type of halogenating
enzymes was detected . -
pubmed
Tryptophan halogenase which catalyses the regioselective chlorination of
tryptophan to
7 - chlorotryptophan can also
chlorinate tryptamine ,
tryptophol ,
indole-3 - acetonitrile , and
3 - methylindole . -
pubmed
Presently , six members have been reported , these are :
neprilysin ,
endothelin - converting enzyme ( ECE )- 1 and
ECE-2 , the
Kell blood group protein ,
PHEX ( product of the
phosphate - regulating gene with homologies to
endopeptidase on the X chromosome ) and
X - converting enzyme (
XCE ) . -
pubmed
In order to identify new members of this important family of peptidases , we designed a
reverse transcriptase - PCR strategy based on conserved
amino acid sequences of
neprilysin ,
ECE-1 and
PHEX . -
pubmed
The recombinant enzyme exhibited
neprilysin - like peptidase activity and was efficiently inhibited by
phosphoramidon and
thiorphan , two inhibitors of
neprilysin . -
pubmed
Both in vitro and in vivo studies of
scavenger receptor class B type I (
SR - BI ) have implicated it as a likely participant in the metabolism of HDL
cholesterol . -
pubmed
The prominent anti - atherogenic effect of moderate
SR - BI expression provides in vivo support for the hypothesis that
HDL functions to inhibit
atherogenesis through its interactions with
SR - BI in facilitating reverse
cholesterol transport . -
pubmed
The amphipathic
polyacid ,
poly ( 2 - ethylacrylic acid ) (
PEAA ) has recently been shown to form fluctuating channels in patch -
clamp measurements of phospholipid bilayers ? -
pubmed
The reaction path starting at the excited n - pi state of
DMD is predicted to occur via a nonadiabatic mechanism , giving
carbene and molecular
dinitrogen ( both in their singlet ground states ) as the main products ; the computed barrier height ( 1.0 kcal mol(-)(1 ) ) agrees well with the experimental estimate of the activation energy in the singlet excited state ( 0.0 - 1.5 kcal mol(-)(1 ) ) . -
pubmed
Linear
IgA bullous dermatosis associated with
vancomycin and disseminated
varicella - zoster infection . -
pubmed
It is known that
NO is formed from
L - arginine by
NO synthase which is localized in neurons in the enteric nervous system . -
pubmed
Although the primary stimulus regulating
vasopressin (
VP ) release is a change in systemic osmolality , other physiological parameters are known to affect
VP secretion or modulate the osmotic control over its release . -
pubmed
The prominant features of
uremic dyslipidemia are an increase in serum
triglyceride levels ( due to elevated
very low density lipoprotein [
VLDL]-remnants and
intermediate - density lipoprotein [
IDL ] ) and low
high - density lipoprotein (
HDL )
cholesterol . -
pubmed
Low - density lipoprotein (
LDL )
cholesterol often is normal , but the
cholesterol may originate from the atherogenic small and dense
LDL subclass (
sdLDL ) . -
pubmed
While
LDL particles undergo a vicious cycle of accumulation and modification , reverse
cholesterol transport is also impaired due to low
lecithin : cholesterol acyltransferase (
LCAT ) and
paraoxonase activity . -
pubmed
Therefore , discoid
HDL particles are structurally altered and hepatic
cholesterol clearance is limited . -
pubmed
Upon a light shift to PSII light ,
PSII , determined as
Cyt b(559 ) per
PBS , decreased rapidly , following the photoinactivation , down to the level a half of that before the light shift , and remained constant . -
pubmed
Photoinactivation of
PSII , and also decrease in
Cyt b(559 ) , was accelerated , but only slightly , by the addition of
chloramphenicol (
CAP ) at a moderate concentration while
CAP at the same concentration significantly suppressed the increment of
PSI determined as P700 . -
pubmed
Mean
glucose levels were significantly higher during the high - dose compared with the low - dose
glucose infusion [ 9.5 + /- 0.8 vs. 6.8 + /- 0.2 mM ( P & lt ; 0.01 ) ] , resulting in increased mean
insulin secretion rates [ ISRs ; 469.1 + /- 43.8 vs. 268.4 + /- 29 pmol / min ( P & lt ; 0.001 ) ] and mean
insulin levels [ 213.6 + /- 46 vs. 67.9 + /- 10.9 pmol / l ( P & lt ; 0.008 ) ] . -
pubmed
Spectral analysis evaluates the regularity of oscillations in
glucose ,
insulin secretion , and
insulin at a predetermined frequency . -
pubmed
Spectral power for
glucose , ISR , and
insulin was reduced during the high - dose
glucose infusion [ 11.8 + /- 1.4 to 7.0 + /- 1.6 ( P & lt ; 0.02 ) , 7.6 + /- 1.5 to 3.2 + /- 0.5 ( P & lt ; 0.04 ) , and 10.5 + /- 1.6 to 4.6 + /- 0.7 ( P & lt ; 0.01 ) , respectively ] . -
pubmed
In conclusion , short - term infusion of high - dose
glucose to obtain
glucose levels similar to those previously seen in
IGT subjects results in reduced spectral power for
glucose , ISR , and
insulin . -
pubmed
Isolation of the
protease nepenthacin . -
pubmed
There was a positive correlation between the net output of
lactate during the aortic cross - clamping period and postoperative Myo /
CAIII ratio . -
pubmed
Trichostatin A (
TSA ; 0 - 5 microM ) , a
histone deacetylase inhibitor , was used to increase histone acetylation and
5 - aza-2 '- deoxycytidine (
5 - aza - dC ; 0 - 5 microM ) , a
DNA methyl - transferase inhibitor , was used to decrease methylation of chromatin in donor cells in an attempt to improve their reprogrammability . -
pubmed
Adult fibroblast cells treated with 1.25 or 5 microM
TSA had elevated
histone H3 acetylation compared to untreated controls . -
pubmed
In addition , 5 methods were used to test the construct validity of the RASS , including correlation with an attention screening examination ( r = 0.78 , P<.001 ) ,
GCS scores ( r = 0.91 , P<.001 ) , quantity of different psychoactive medication dosages 8 hours prior to assessment ( eg ,
lorazepam : r = - 0.31 , P<.001 ) , successful extubation ( P = .07 ) , and bispectral electroencephalography ( r = 0.63 , P<.001 ) . -
pubmed
Molecular dissection of the
S - adenosylmethionine - binding site of
phosphatidylethanolamine N - methyltransferase . -
pubmed
Phosphatidylethanolamine N - methyltransferase (
PEMT ) is a quatrotopic membrane protein that catalyzes the conversion of
phosphatidylethanolamine to
phosphatidylcholine through three sequential methylation reactions . -
pubmed
Homocysteine is generated by the hydrolysis of
S - adenosylhomocysteine , which is also a product of the
PEMT reaction . -
pubmed
To gain insight into the
PEMT transmethylation reaction and the mechanism by which
PEMT regulates
homocysteine levels , we sought to define residues that are required for binding of the
methyl group donor ,
S - adenosylmethionine (
AdoMet ) . -
pubmed
Analysis of the
AdoMet binding ability of mutant recombinant
PEMT derivatives established that residues
Gly100 and
Glu180 are essential for binding of the
AdoMet moiety . -
pubmed
A model positions two separate
AdoMet - binding motifs of
PEMT in close proximity at the external leaflet of the endoplasmic reticulum membrane . -
pubmed
Maximum growth of the tissue , as measured by dry weight , with the formation of
collagen protein , based on the estimation of
hydroxyproline , was obtained in periods of up to 6 days . -
pubmed
Appreciable amounts of protein - bound
hydroxyproline were formed during the first 24 hour growth period , but electron microscopy of portions of the same cultures failed to demonstrate the presence of any typical
collagen fibrils . -
pubmed
The subsequent formation of many characteristic
collagen fibrils was not associated with a significant rise in the mean
hydroxyproline content of the tissue . -
pubmed
It is suggested that collagen - forming cells synthesise and secrete a
hydroxyproline - rich precursor of protein or large peptide nature , which subsequently becomes directly transformed into typical
collagen fibrils . -
pubmed
[ Effect of
histaminase on the secretion of gastric juice induced by
histamine ] . -
pubmed
Clone 7 - 3 - 5 - 5 was sequenced and found to contain a 672bp ORF encoding a 224
amino acid protein having a 19
amino acid signal sequence on the N - terminus and a 15
amino acid C - terminal
GPI anchor site . -
pubmed
Those agents that inhibit
beta - adrenergic receptors ( class
II beta - blockers ,
sotalol , and
amiodarone ) have been demonstrated to be successful prophylaxis against
postoperative AF . -
pubmed
Preincubation with
LY-294002 , a
PI 3 - kinase inhibitor , or expression of dominant - negative
Akt prevented
ANG II - stimulated increase in
VEGF translation . -
pubmed
AIM : Despite the belief that
silica (
Si ) is an inert and non - toxic ingredient , latest studies indicated that it is a potent mitochondria activator and
Si - induced ROS generation is involved in the inflammatory reactions of silicotic lungs . -
pubmed
GTP cyclohydrolase I (
GTPCH1 ) catalyzes the conversion of
GTP to
dihydroneopterin 3 '- triphosphate . -
pubmed
These results suggest that
8 - oxo - GTP is a transition state analogue of
GTPCH1 . -
pubmed
We have solved the X - ray crystal structures of
tGTPCH1 complexed with
8 - oxo - GTP and
8 - oxo - dGTP at 2.0 and 1.8 A resolution , respectively , as well as the free form of the
enzyme at 2.2 A resolution . -
pubmed
In the structure of
tGTPCH1 complexed with
8 - oxo - GTP or
8 - oxo - dGTP , the
oxygen atoms at
O8 of the
8 - oxoguanine groups , together with residues
Cys108 ,
His111 and
Cys179 , are coordinated to the
zinc ion . -
pubmed
Superimpositioning of the structures indicates the
imidazole ring of
His110 is rotated , implying concomitant proton transfer to the
ribose ring
O4 ' . -
pubmed
Both
PrP(C ) and
PrP(Sc ) have
glycosylphospatidylinositol ( GPI ) anchors through which the protein is tethered to cell membranes . -
pubmed
In order to study the effects of membranes on the structure of
PrP , we synthesized a GPI anchor mimetic ( GPIm ) , which we have covalently coupled to a genetically engineered
cysteine residue at the C - terminus of recombinant
PrP. -
pubmed
We show that the structure of
PrP - GPIm reconstituted in
phosphatidylcholine and raft membranes resembles that of
PrP , without a
GPI anchor , in solution . -
pubmed
Increases in astrocytic
Ca ( 2 + ) were a function of the frequency of stimulation , occurred within several seconds and were inhibited by
metabotropic glutamate receptor antagonists . -
pubmed
To distinguish between synaptic input and output , local synaptic activity in cortical layer 2 was silenced by iontophoresis of
AMPA and
NMDA receptor antagonists . -
pubmed
Mutations of the
BCR -
ABL kinase domain are a common mechanism of resistance to
imatinib in
chronic myeloid leukemia . -
pubmed
By multivariate analysis , factors associated with development of mutations were older age ( P=0.026 ) prior
interferon therapy ( P=0.026 ) , and accelerated phase or
blast phase at time of
imatinib failure ( P=0.001 ) . -
pubmed
The crystal structure determined by Rietveld analysis with the NPD data is of a distorted
rutile type , similar to that of
MoO2 ; space group
P21 / c , a = 5.6891(1 ) , b = 4.7546(1 ) , c = 5.5195(1 ) A , and beta = 121.453(1 ) degrees . -
pubmed
The
NPD analysis also establishes a new neutron scattering length of 6.00(3 ) fm for
99Tc . -
pubmed
The
Tc - Tc bond was found to be 2.622(1 ) A from
NPD profile analysis and 2.59 A from first - principles DFT calculations . -
pubmed
The
NPD results supported by the DFT calculations suggest that the filling of antibonding orbitals and the influence of the crystal field stabilization of the d3 Tc cations lead to more regular
TcO6 octahedra and diminish the metal - metal bond strength compared with closely related
oxides such
MoO2 and
WO2 . -
pubmed
Synthesis of
pyrrolo [ 2,3 - d ] pyridazinones as potent , subtype selective
PDE4 inhibitors . -
pubmed
A series of
pyrrolo [ 2,3 - d ] pyridazinones was synthesized and tested for their inhibitory activity on
PDE4 subtypes A , B and D and selectivity toward
Rolipram high affinity binding site ( HARBS ) . -
pubmed
Moreover the ratio HARBS /
PDE4B was particularly favourable for 9e ( 147 ) , suggesting that the best arranged groups around the
pyrrolopyridazinone core are an
isopropyl at position-1 , an
ethoxycarbonyl at position-2 , together with an
ethyl group at position-6 . -
pubmed
ParF , which exemplifies one clade of the ubiquitous
ParA superfamily of segregation proteins , polymerizes extensively in response to
ATP binding . -
pubmed
Here , we establish that the
ParF homologues of pTAR and
pB171 filament modestly with
ATP , and that
nucleotide hydrolysis is not required for this polymerization , which is more prodigious when the cognate
CBF is also present . -
pubmed
By contrast , the
ParF homologue of plasmid pVT745 did not respond appreciably to
ATP alone , but polymerized extensively in the presence of both its cognate
CBF and
ATP . -
pubmed
The co - factors also stimulated
nucleotide - independent polymerization of cognate
ParF proteins . -
pubmed
2 - Aminoresorcinol is a potent
alpha - glucosidase inhibitor . -
pubmed
Glutamic acid decarboxylase (
GAD ) is the rate - limiting enzyme for
gamma - aminobutyric acid (
GABA ) biosynthesis . -
pubmed
To overcome these problems , this paper evaluates resistance and recovery , quantified by using a relative soil stability index ( RSSI ) , of the
beta - glucosidase and
protease activities towards an additional heat disturbance ( 17 h at 60 degrees C ) in soils where soil organic matter , pH and
Cu content were modified in a factorial setup . -
pubmed
Chemical analyses ( dissolved
Cu ,
pCu ( 2 + ) , dissolved organic
carbon , pH ) were performed both before the heat - perturbation and after the
enzyme activity monitoring period . -
pubmed
beta - glucosidase RSSI scores were scarcely affected by
copper , making it inappropriate for evaluating
copper - induced stress to soils . -
pubmed
Thus , the
protease RSSI score seems a good indicator for soil health relative to
copper contamination given that all samples were affected by the presence of
copper and high correlations were observed between RSSI scores and the different
copper forms . -
pubmed
Effect of charge distribution over a
chlorophyll dimer on the redox potential of P680 in
photosystem II as studied by density functional theory calculations . -
pubmed
The effect of charge distribution over a
chlorophyll dimer on the redox potential of P680 in
photosystem II was studied by density functional theory calculations using the P680 coordinates in the X - ray structure . -
pubmed
The novel
HSP90 inhibitor
STA-9090 exhibits activity against
Kit - dependent and -independent
malignant mast cell tumors . -
pubmed
MATERIALS AND METHODS : BMCMCs , cell lines , and fresh malignant mast cells were treated with
STA-9090 ,
17 - AAG , and
SU11654 and evaluated for loss in cell viability , cell
death , alterations in
HSP90 and
Kit expression / signaling , and
Kit mutation . -
pubmed
RESULTS : Treatment of BMCMCs , cell lines , and fresh malignant cells with
STA-9090 induced growth inhibition , apoptosis that was
caspase-3 / 7 - dependent , and downregulation of
phospho / total
Kit and
Akt , but not
extracellular signal - regulated kinase (
ERK ) or
phosphoinositide-3 kinase (
PI-3 K ) . -
pubmed
Furthermore ,
STA-9090 exhibited superior activity to
17 - AAG and
SU11654 , and was effective against malignant mast cells expressing either WT or mutant
Kit . -
pubmed
CONCLUSIONS :
STA-9090 exhibits broad activity against mast cells expressing WT or mutant
Kit , suggesting it may be an effective agent in the clinical setting against
mast cell malignancies . -
pubmed
Synthesis of
collagen - like sequential polypeptides containing
O - phospho - L - hydroxyproline and preparation of electrospun composite fibers for possible dental application . -
pubmed
A synthetic route is described for
collagen - like polypeptides constructed from
O - phospho - L - hydroxyproline [
Hyp ( PO ( 3 ) H ( 2 ) ) ] residues . -
pubmed
The initial rate and inhibitor analyses revealed that the mechanism of binding of three substrates ,
glutamate ,
ATP , and
tubulin , to the enzyme was a random sequential pathway . -
pubmed
Articular chondrocytes were subjected to several cell culture passages and treated with or without a
Notch inhibitor ,
N -[ N -( 3 , 5 - difluorophenacetyl - L - alanyl )]- S - phenylglycine t - Butyl Ester (
DAPT ) . -
pubmed
Furthermore , even though this
TEM is driven by
CXCR4 , the
CXCR7 ligand
CCX771 is substantially more potent at inhibiting it than the
CXCR4 antagonist
AMD3100 , which is more than 100 times weaker at inhibiting
TEM when compared with its ability to block bare filter chemotaxis . -
pubmed
Remarkably , the synthetic
CXCR7 ligand
CCX771 also potently stimulates
beta - arrestin2 recruitment to
CXCR7 , with greater potency and efficacy than the endogenous
chemokine ligands . -
pubmed
CXCR7 ligands , such as the
chemokine CXCL11 and the newly described synthetic molecule
CCX771 , may represent novel therapeutic opportunities for the control of such cells . -
pubmed
In vitro , neurite outgrowth triggered by the Lewis(x-)carrying adhesion molecule
CD24 was abolished alike by the
Lewis ( x ) glycan and the glycomimetic peptide , while no influence of the glycomimetic peptide was seen in regeneration . -
pubmed
If germination problems are bypassed by the addition of
gibberellins , the seedlings of the quintuple
phytochrome mutant exposed to red light produce
chlorophyll , indicating that
phytochromes are not the sole red - light photoreceptors , but they become developmentally arrested shortly after the cotyledon stage . -
pubmed
We conclude that the degradation of the
V - ATPases , electrogenic
proton pumps on the
tonoplast and the membranes of the Golgi- dependent secretory pathway , may be the cause for the cessation of growth in turgid coleoptiles and the associated loss of
auxin sensitivity . -
pubmed
Steaks were individually packaged in either vacuum (
VP ) , high -
oxygen MAP ( HIOX ; 80 %
O ( 2 )+20 %
CO ( 2 ) ) , or
carbon monoxide MAP (
CO ; 0.4 %
CO+19.6 %
CO ( 2 )+80 %
N ( 2 ) ) , and stored for 0 , 5 , or 9 days at 1 C. At the end of storage , surface and internal colour ( visual and instrumental ) was measured on raw steaks . -
pubmed
Interior cooked redness decreased ( P<0.05 ) during storage for steaks in
VP and HIOX , whereas it was stable for steaks in
CO . -
pubmed
Besides hydrophobic interactions , the ligand
9 - cis retinoic acid interacts with
RXRgamma through a
hydrogen bond with
Ala106 , a salt bridge with
Arg95 and the pi - pi interactions with
Phe217 and
Phe218 . -
pubmed
The binding modes exhibit some similarities among
RXRs , such as the interactions with
Arg95 and
Ala106 . -
pubmed
Nonetheless , owing to the absence of
Ile47 ,
Cys48 ,
Ala50 ,
Ala51 and residues 225~237 in the active site , the binding pocket in
RXRgamma is two times larger than those of
RXRalpha and
RXRbeta . -
pubmed
Meanwhile , spatial effects of
Trp84 ,
Arg95 ,
Ala106 ,
Phe217 and
Phe218 help to create a differently shaped binding pocket as compared to those of
RXRalpha and
RXRbeta . -
pubmed
GDF-15 levels correlated positively with estimated right ventricular
systolic pressure on echocardiogram and plasma levels of the
amino terminal propeptide form of
brain natriuretic peptide . -
pubmed
There was an inverse correlation between circulating
GDF-15 and diffusing capacity of the lung for
carbon monoxide ( Dlco ) and a positive correlation with the FVC to Dlco ratio on
pulmonary function test . -
pubmed
Upon subjecting the patterned
silicon surfaces to solvothermal conditions , MOF thin films were obtained and characterized subsequently by
AFM , SEM , and grazing - incidence XRD ( GIXRD ) . -
pubmed
INTRODUCTION :
Sirolimus is a potent immunosuppressant with a mechanism of action different from
calcineurin inhibitors ( CNIs ) . -
pubmed
METHODS : Between 2007 and 2009 , 250 newly diagnosed , histologically confirmed
ccRCC cases and 299 sex- , age - matched healthy controls provided complete information including consumption of tea and
alcohol , smoking , occupational exposure ,
body mass index (
BMI ) ,
hypertension ,
diabetes , and
urolithiasis by
face - to - face interview in Shanghai . -
pubmed
Cannabidiol has been reported as a
GPR55 antagonist in the literature to
GPR55 activation by
O1062 , but this could not be confirmed our laboratory ( Dr. Abood & amp ; Dr. Barak ) . -
pubmed
There are no other known small molecule antagonists of
GPR55 reported to date , only the
CB1 inverse agonist / antagonists
SR141716A ( 3.9 muM ) and
AM251 ( 9.6 muM ) . -
pubmed
We have identified 3 potent and selective antagonists for
GPR55 , that represent 3 different chemical core scaffolds : 1 ) a
quinoline aryl sulfonamide ,
ML193 (
CID1261822 ) with a 221 nM potency for
GPR55 and & gt;145 - fold , & gt;27 - fold and & gt;145 - fold antagonist selectivity against
GPR35 ,
CB1 and
CB2 , respectively and & gt;145 - fold agonist selectivity against all of these counter - receptors ; 2 ) a
thienopyrimidine ,
ML192 (
CID1434953 ) with 1080 nM potency for
GPR55 and & gt;45 - fold antagonist and agonist selectivity against
GPR35 ,
CB1 and
CB2 ; and 3 ) a
piperadinyloxadiazolone ,
ML191 ( 23612552 ) with 160 nM potency for
GPR55 and & gt;100 - fold selectivity against
GPR35 ,
CB1 and
CB2 . -
pubmed
When
Taxol was locally injected near the germinal vesicle ( GV ) or the mitotic apparatus during meiosis I ,
PB formation was inhibited as mentioned below . -
pubmed
In the oocytes , which were injected with
Taxol after spindle formation , the spindle became large , and then the volume of the first
PB also increased more than that of the control . -
pubmed
Tissular
hypoxia and its impact were quantified using
pimonidazole immunostaining and mRNA of
hypoxic inducible factor ,
vascular endothelial growth factor receptors 1 and 2 ,
Tie-2 ,
endothelial nitric oxide synthase , and
inducible nitric oxide synthase . -
pubmed
Fasting plasma
glucose ( FPG ) , 2 - h plasma
glucose ( 2hPG ) ,
HbA1c ,
insulin resistance ( HOMA2 - IR ) and beta - cell function ( HOMA2 - B ) were considered at 5 - year follow - up . -
pubmed
We find that cells depleted of
RECQL5 are sensitive to oxidative stress , accumulate endogenous DNA damage , and increase the cellular
poly ( ADP - ribosyl )ate response . -
pubmed
We recently identified a UVA phototransduction pathway in melanocytes that is mediated by
G protein - coupled receptors and leads to rapid
calcium mobilization . -
pubmed
pullorum , denoted
HPMG439 /
HPMG440 , is involved in the control of
nitrogen metabolism by regulating the expression of
glutamate dehydrogenase , an
AmtB ammonium transporter and a
PII protein . -
pubmed
Glucagon - like peptide-1 receptor agonist
liraglutide inhibits
endothelin-1 in endothelial cell by repressing
nuclear factor - kappa B activation . -
pubmed
To ascertain the role of
NF - kappaB activation in the altered expression of
ET-1 and
eNOS , we treated HUVECs with
phorbol 12 - myristate 13 - acetate (
PMA ) . -
pubmed
PMA activated
NF - kappaB and reversed the effects of
liraglutide on
eNOS and
ET-1 expression . -
pubmed
The effects of
PMA on
eNOS and
ET-1 expression were reproduced in experiments wherein cells were treated with
TNF - alpha . -
pubmed
PMA increased
IL-6 generation , and this effect was blocked by
liraglutide . -
pubmed
Genistein induces
oestrogen receptor - alpha gene expression in osteoblasts through the activation of
mitogen - activated protein kinases /
NF - kappaB /
activator protein-1 and promotes cell mineralisation . -
pubmed
Oestrogen and
oestrogen receptors (
ER ) play critical roles in the maintenance of bone remodelling . -
pubmed
Sequentially ,
genistein increased the translocation of
NF - kappaB and
c - Jun from the cytoplasm to the nucleus . -
pubmed
Therefore , the present study showed that
genistein can induce
ERalpha gene expression via the activation of
MAPK /
NF - kappaB /
activator protein-1 and accordingly stimulates differentiation - related gene expressions and osteoblast mineralisation . -
pubmed
Pre - treatment with the inhibitor ,
CRP - i2 , resulted in a significant reduction in
CRP induced
superoxide anion ,
NFkappab activity and biomarkers of
inflammation . -
pubmed
[ Detection of
tumors in the central zone of the prostate with
11C - Choline PET /
CT ] . -
pubmed
In both ,
( 11 ) C - Choline PET /
CT allowed detection of the
tumor focus in the central zone of the prostate , with negative complementary diagnostic test and biopsies . -
pubmed
Part
II continues this research by delving deeper into the
amino acid ,
fatty acid and mineral components . -
pubmed
Nanoporous (
NP )
gallium nitride (
GaN ) as a new class of
GaN material has many interesting properties that the conventional
GaN material does not have . -
pubmed
In this paper , we focus on the mechanical properties of
NP GaN , and the detailed physical mechanism of porous
GaN in the application of liftoff . -
pubmed
A decrease in elastic modulus and hardness was identified in
NP GaN compared to the conventional
GaN film . -
pubmed
The promising application of
NP GaN as release layers in the mechanical liftoff of
GaN thin films and devices was systematically studied . -
pubmed
A phase diagram was generated to correlate the initial
NP GaN profiles with the as - overgrown morphologies of the
NP structures . -
pubmed
The fracture toughness of the
NP GaN release layer was studied in terms of the voided - space - ratio . -
pubmed
It is shown that the transformed morphologies and fracture toughness of the
NP GaN layer after overgrowth strongly depends on the initial porosity of
NP GaN templates . -
pubmed
Exposure to chemotherapy prior to cell line initiation correlated with drug resistance of EFT cell lines in 5/8 tested agents at clinically achievable concentrations (
CAC ) or the lower tested concentration ( LTC ): (
cyclophosphamide ( as
4 - HC ) and
doxorubicin at
CAC ,
etoposide ,
irinotecan ( as
SN-38 ) and
melphalan at LTC ; P<0.1 for one agent , and P<0.05 for four agents . -
pubmed
Neutron diffraction shows that the
hydrides are best described as edge bridging rather than
face bridging . -
pubmed
By MD simulations we further investigated how
VWD 2A / IID mutations might alter the ability of
VWF to form
carboxy - terminal dimers . -
pubmed
PK 11195 , an
isoquinoline analogue , is one of the ligands of highest
TSPO binding affinity . -
pubmed
The main
water channel in the brain ,
aquaporin-4 (
AQP4 ) is involved in maintaining homeostasis and
water exchange in the brain . -
pubmed
In addition we used freeze - fracture
electron microscopy to detect
AQP4 in the form of orthogonal arrays of particles ( OAPs ) on the ultrastructural level . -
pubmed
The
cytochrome P450 monooxygenases are known to play a major role in
pyrethroid resistance , by means of increased rate of insecticide detoxification as a result of their overexpression . -
pubmed
leaves and roots was examined against
benzyloxyresorufin O - debenzylation ( BROD ) of
CYP6AA3 and
CYP6P7 . -
pubmed
Docking of
flavones onto
enzyme homology models reinforced relationships on
flavone structures and inhibition modes . -
pubmed
The
SE - UBR group increased time spent in
LPA by +20.68 + -29.30 minutes / day and the other groups decreased time spent in
LPA by -22.43 + -47.88 minutes / day and -25.73 + -51.76 minutes / day . -
pubmed
The Pn , stomatal limitation value ( Ls ) , maximal photochemical efficiency of PS
II ( Fv / Fm ) , photochemical quenching coefficient ( q(p ) ) , content of
malondialdehyde (
MDA ) , relative electric conductivity and
superoxide dismutase (
SOD ) activity of leaves were very significantly correlated with the drought resistance coefficient . -
pubmed
The self - propelled Janus
silica micromotor catalytically decomposes a
hydrogen peroxide fuel and moves along the direction of the catalyst
face at a speed of 126.3 mum s(-1 ) . -
pubmed
Sequence analysis of 5 isolates from the 4 provinces showed that they belonged to a relatively independent cluster that shared 2 insertions of a single
amino acid in the
gE gene and 1 insertion of 7
amino acids in the
gC gene . -
pubmed
The title compounds ,
C13H8ClN3O6 , ( I ) , and
C13H9N3O6 , (
II ) , differ in the orientation of the two
aromatic rings . -
pubmed
The two
nitro groups are essentially coplanar with the attached
benzene rings , as indicated by the dihedral angles of 1.4 ( 2 ) and 2.3 ( 2 ) in ( I ) , and 4.96 ( 18 ) and 5.4 ( 2 ) in (
II ) . -
pubmed
By following our previous introduction of
PRM to targeting
histone modifications ( Tang , H. ; Fang , H. ; Yin , E. ; Brasier , A. R. ; Sowers , L. C. ; Zhang , K. Multiplexed parallel reaction monitoring targeting
histone modifications on the QExactive mass spectrometer . -
pubmed
All received isonitrogenous , isocaloric
PN [ 1.5 g / kg / d
amino acids ( AAs ) and energy at 1.3x estimated basal energy expenditure ] . -
pubmed
Controls ( n = 75 ) received standard GLN - free
PN (
STD -
PN ) ; the GLN group ( n = 75 ) received
PN containing
alanyl - GLN dipeptide ( 0.5 g / kg / d ) , proportionally replacing AA in
PN ( GLN -
PN ) . -
pubmed
There were 11 hospital
deaths ( 14.7 % ) in the
GLN -
PN group and 13
deaths in the
STD -
PN group ( 17.3 % ; difference , -2.6 % ; 95 % confidence interval , -14.6 % to 9.3 % ; P = 0.66 ) . -
pubmed
The 6 - month cumulative mortality was 31.4 % in the
GLN -
PN group and 29.7 % in the
STD -
PN group ( P = 0.88 ) . -
pubmed
Incident
bloodstream infection rate was 9.6 and 8.4 per 1000 hospital days in the
GLN -
PN and
STD -
PN groups , respectively ( P = 0.73 ) . -
pubmed
Orthovanadate ( OVA ) , a
protein tyrosine phosphatase inhibitor , induces vasoconstriction in a
Rho kinase - dependent manner . -
pubmed
OVA - induced constriction of mesenteric arterial rings treated with
N ( G )- nitro - L - arginine methyl ester (
L - NAME , 0.1 mM ) , a
nitric oxide synthase inhibitor , was significantly blocked by the
Rho kinase inhibitor
Y-27632 (
R -(+)- trans - N -( 4 - pyridyl )- 4 -( 1 - aminoethyl )- cyclohexanecarboxamide , 10 microM ) ,
extracellular signal - regulated kinase 1 and 2 (
Erk1 / 2 ) inhibitor
FR180204 (
5 -( 2 - phenyl - pyrazolo [ 1,5 - a ] pyridin-3 - yl )- 1H - pyrazolo [ 3,4 - c ] pyridazin-3 - ylamine , 10 microM ) ,
Erk1 / 2 kinase (
MEK ) inhibitor
PD98059 (
2 '- amino-3 '- methoxyflavone , 10 microM ) ,
epidermal growth factor receptor (
EGFR ) inhibitor
AG1478 (
4 -( 3 - chloroanilino )- 6,7 - dimethoxyquinazoline , 10 microM ) , and
Src inhibitor
PP2 (
4 - amino-3 -( 4 - chlorophenyl )- 1 -( t - butyl )- 1H - pyrazolo [ 3,4 - d ] pyrimidine , 3 microM ) . -
pubmed
However , the
myosin light chain kinase inhibitor
ML-7 (
1 -( 5 - iodonaphthalene-1 - sulfonyl )- homopiperazine , 10 microM ) did not affect OVA - induced constriction . -
pubmed
The degree of formylation evaluated by
( 1 ) H NMR spectra was varied from
14.1 % to 37.2 % as a function of the reaction temperature . -
pubmed
Effects of specific
imidazoline receptor (
IR ) antagonists on the
agmatine - induced relaxation were studied . -
pubmed
The relaxation was inhibited by
BU224 , a selective
I2 IR antagonist ; but not by
efaroxan (
I1 IR antagonist ) or KU14R (
I3 IR antagonist ) . -
pubmed
Moreover , the
agmatine - induced relaxation was attenuated by
glibenclamide ( inhibitor of
KATP channel ) and
H-89 ( inhibitor of
protein kinase A ) , but enhanced by
3 - isobutyl-1 - methylxanthine (
IBMX , inhibitor of
cyclic AMP phosphodiesterase ) . -
pubmed
BACKGROUND :
Everolimus (
EVL)-based immunosuppressive strategies may permit the reduction of
calcineurin inhibitors ( CNI ) and their side effects , while offering a safe and efficient treatment . -
pubmed
omega - Imidazolyl - and omega - Tetrazolylalkylcarbamates as Inhibitors of
Fatty Acid Amide Hydrolase : Biological Activity and in vitro Metabolic Stability . -
pubmed
Fatty acid amide hydrolase (
FAAH ) is a
serine hydrolase that terminates the analgesic and anti - inflammatory effects of endocannabinoids such as
anandamide . -
pubmed
Herein , structure - activity relationship studies on a new series of
aryl N -( omega - imidazolyl - and omega - tetrazolylalkyl ) carbamate inhibitors of
FAAH were investigated . -
pubmed
Moreover , it was found that highly active
pyridin-3 - yl carbamates reacted with
albumin , which led to covalent
albumin adducts . -
pubmed
Most phenotypic correlations among
Ca , P ,
Mg , and K concentrations were positive and low ( 0.09 - 0.16 ) , whereas the correlation between
P and Mg was negative and low ( -0.16 ) . -
pubmed
In this study , the differences in formation of
Cu - mediated metal - organic coordination networks based on two
pyridyl- and
cyano - bearing free - base
porphyrins on
Ag ( 111 ) are elucidated by use of low - temperature scanning tunneling microscopy (
STM ) . -
pubmed
Prior to the frozen donor egg transfer cycle ,
prednisone and intravenous
immunoglobulin G (
IVIg ) treatment was initiated and patient achieved dichorionic diamniotic twin pregnancies . -
pubmed
The present study was carried out to evaluate the capability of a
polyethylene glycol mimetic technology as a biological alternative to improve pharmaceutical properties of
human recombinant EPO . -
pubmed
In silico models of
EPO fused to 200
amino acids of
proline ,
alanine , and
serine (
PAS ) were initially generated and assessed by molecular dynamic ( MD ) simulation . -
pubmed
Preclinical predictors that the orthosteric
mGlu2 / 3 receptor antagonist
LY3020371 will not engender
ketamine - associated
neurotoxic , motor , cognitive , subjective , or abuse - liability - related effects . -
pubmed
The novel
mGlu2 / 3 receptor antagonist ,
LY3020371 , has been shown to produce antidepressant - like effects comparable to that of the clinically - effective antidepressant
ketamine . -
pubmed
The robust
polylysine protective shell makes the nanoparticles very stable under conditions of chemical etching , in the presence of
glutathione , and at different pH values , without gold core deshielding or alteration of the
SPR - like band . -
pubmed
Susceptibility to
rheumatoid arthritis (
RA ) is associated with
HLA - DRB1 alleles coding a 5 -
amino acid sequence motif called the shared epitope (
SE ) . -
pubmed
The results also showed that CRME can reduce the levels of
IL-1 ,
IL-6 ,
TNF - alpha , and
PGE2 and inhibit the expression of
NF - kappaB p65 . -
pubmed
Compounds 3 and 7 exhibited anti - inflammatory activity through lowering the levels of
TNF - alpha while 1 , 3 and 5 decreased the levels of
neopterin better than the positive controls . -
pubmed
The voltage - sensing residues in
potassium channels have been extensively characterized , but current functional approaches do not allow a distinction between the electrostatic and steric contributions of the
arginine side chain . -
pubmed
Here we use chemical misacylation and in vivo nonsense suppression to encode
citrulline , a neutral and nearly isosteric analogue of
arginine , into the voltage sensor of the
Shaker potassium channel . -
pubmed
In contrast , substitution of the fourth
S4 arginine with
citrulline causes substantial changes in the conductance - voltage relationship and the kinetics of the channel , which suggests that a positive charge is required at this position for efficient voltage sensor deactivation and channel closure . -
pubmed
In Functional magnetic resonance imaging ( fMRI ) , the blood
oxygen level dependent ( BOLD ) signal is modeled as a convolution of the hemodynamic response function (
HRF ) and the unmeasured latent neural signal . -
pubmed
Estrogen induces cell proliferation by promoting
ABCG2 - mediated efflux in
endometrial cancer cells . -
pubmed
The present study aimed at understanding the regulatory mechanism underlying
17 - beta - estradiol ( E2)-induced cell proliferation under the control of
ABCG2 . -
pubmed
E2 - induced cell proliferation was inhibited by
reserpine , an inhibitor of
ABCG2 , and the
ABCG2 siRNA treatment . -
pubmed
Mitochondrial ultrastructure was observed by transmission electron microscope and
adenosine triphosphate (
ATP ) content ,
sodium potassium ATPase (
Na / K - ATPase ) activity , and mitochondrial respiratory chain complexes activities in hippocampus and gastrocnemius muscle were measured at the 14th and 42nd day . -
pubmed
CUMS reduced
ATP content in hippocampus , decreased
Na / K - ATPase activity and
respiratory chain complex I , III , and IV activities in hippocampus and gastrocnemius muscle , and damaged mitochondrial ultrastructure of hippocampus and gastrocnemius muscle . -
pubmed
XPKYF could partly reverse alterations in
ATP ,
Na / K - ATPase , and respiratory chain complexes of hippocampus and gastrocnemius muscle and protect mitochondria from ultrastructural damage . -
pubmed
Using recombinant adenoviruses with
amino acid substitutions or a deletion in the ribonucleoprotein motif the interaction of
E1B 55kDa with RNA was found to correlate with timely and efficient viral DNA replication and viral late mRNA accumulation and splicing . -
pubmed
Development of chitosan /
glycerophosphate /
collagen thermo - sensitive hydrogel for endoscopic treatment of mucosectomy - induced
ulcer . -
pubmed
In this study , we developed the chitosan /
beta - glycerophosphate /
collagen (
CS /
GP / Col ) thermo - sensitive systems to satisfy the requirements for the endoscopic treatment for
ESD - induced
ulcer . -
pubmed
In - silico design , ADMET screening , MM - GBSA binding free energy of some novel
isoxazole substituted
9 - anilinoacridines as
HER2 inhibitors targeting
breast cancer . -
pubmed
OBJECTIVE : A progression of novel
isoxazole substituted
9 - anilinoacridines(1a - z ) were structured by in - silico technique for their
HER2 inhibitory movement . -
pubmed
RESULTS : Many compounds indicated solid hydrophobic communications and
hydrogen holding associations to hinder
HER2 . -
pubmed
CONCLUSION : The outcomes reveals that , this examination gives proof to thought of profitable ligands in
isoxazole substituted
9 - anilinoacridines as potential
HER2 inhibitor and the compounds , 1s , x , v , a , j , r with noteworthy Glide scores may deliver critical enemy of bosom malignant growth movement for further examinations may demonstrate their remedial potential . -
pubmed
Here , we tested the hypothesis that sedentary conditions , a major risk factor for
CVD , increase
GABAA receptors in RVLM , including its rostral extension ( RVLMRE ) , both of which contain bulbospinal
catecholamine (
C1 ) and non - C1 neurons . -
pubmed
These results suggest a complex interplay between
GABAA receptor expression by spinally - projecting
C1 and non - C1 neurons and sedentary versus physically active conditions . -
pubmed
The released levels of phosphorylated -
HSP27 was correlated with the levels of phosphorylated -
HSP27 stimulated by
ADP or
collagen . -
pubmed
The levels of
ADP - induced phosphorylated -
HSP27 were correlated with those of both phosphorylated -
protein kinase B (
Akt ) and phosphorylatd -
p38 mitogen - activated protein kinase ; however , the levels of phosphorylated -
HSP27 stimulated by
collagen were correlated with phosphorylated -
Akt levels only . -
pubmed
The ED50 value of
ADP on the
platelet aggregation in
OSAS ( 1.067 + - 0.128 muM ) was lower than that in healthy subjects ( 1.778 + - 0.122 muM ) and was inversely correlated with both the value of minimum SpO2 and the released level of phosphorylated -
HSP27 stimulated by
ADP . -
pubmed
Finally , we found
aspirin alone had a lower live birth rate compared to
LMWH plus
aspirin ( OR=0.37 ; 95%CrI , 0.17 , 0.71 ) , and
UFH plus
aspirin showed a higher live birth rate than
aspirin alone ( OR= 2.63 ; 95%CrI , 1.04 , 5.39 ) in
NMA , Treating with UFH plus
aspirin or
LMWH plus
aspirin did not have difference on live birth . -
pubmed
Furthermore , LDA alone resulted in a lower
birth weight compared to
heparin plus
aspirin , while higher
birth weight was found when compared UFH plus
aspirin to
LMWH plus
aspirin ( MD= 895.40 ; 95%CrI , 817.40 , 988.57 ) in
NMA . -
pubmed
Biocompatible long - circulating
star carboxybetaine polymers . -
pubmed
Here , we prepared four
star carboxybetaine polymers of different molecular weights via atom transfer radical polymerization ( ATRP ) from a beta - cyclodextrin ( beta -
CD ) initiator for investigating the biocompatibility of
carboxybetaine polymer , a typical zwitterionic polymer . -
pubmed
The internalization of the
star carboxybetaine polymers by macrophage cells was a relatively slow process . -
pubmed
The high cell viability in the presence of
star carboxybetaine polymers up to 2 mg mL-1 was maintained . -
pubmed
The hemolytic activity of the
star carboxybetaine polymers at 5 mg mL-1 was almost undetectable . -
pubmed
The anti -
obesity effects of
baicalin might be due to the up - regulation of
solute carrier family 2 member 1 (
SLC2A1 ) and down - regulation of
tumor necrosis factor (
TNF ) ,
nuclear factor kappa B subunit 1 (
NFKB1 ) ,
sterol regulatory element binding transcription factor 1 (
SREBF1 ) ,
peroxisome proliferator activated receptor gamma and
caspase 3 (
CASP3 ) . -
pubmed
Sirtuins ( SIRTs ) , a family of
NAD+-dependent deacetylase , play an important role in regulating mitochondrial homeostasis and antioxidant defense by deacetylating transcription factors and enzymes such as
PGC-1alpha ,
p65 ,
GCN5 , and
SOD2 . -
pubmed
However , age - related DNA damage and increased
SASP activate
PARP-1 and
CD38 , the enzymes competing with
SIRTs for
NAD + . -
pubmed
Aging increased the protein content of
PARP-1 and
CD38 , whereas decreased
NAD + levels in both skeletal and heart muscles . -
pubmed
Taken together , our data suggest that despite the upregulation of SIRTs , aged muscles suffered from
NAD + deficit partly due to the competition of elevated
CD38 and
PARP-1 . -
pubmed
A great preference for ordered binding is supported by stopped - flow double mixing experiments such that premixed
CrAT with
acetyl - CoA or
CoA demonstrated a biphasic decrease in initial rate that produces about a 100 - fold attenuation in catalysis . -
pubmed
Double mixing experiments also revealed that the
CrAT initial rate is inhibited by 50 % in approximately 8 s by either
acetyl - CoA or
CoA premixing . -
pubmed
Analysis of available
CrAT structures support a substrate conformational change between
acetyl - CoA /
CoA binary versus ternary complexes . -
pubmed
Enzyme LIP05 , belonging to the
alpha / beta hydrolase family , was identified to synthesize short - chain
fatty acid esters under aqueous phase . -
pubmed
After deleting the lid domain of
LIP05 , the synthesis of
ethyl pentanoate ,
ethyl hexanoate ,
ethyl octanoate , or
ethyl decanoate was achieved . -
pubmed
Economic evaluation of adjuvant
trastuzumab therapy for
HER2 - positive early - stage
breast cancer : systematic review and quality assessment . -
pubmed
INTRODUCTION : As the availability of new economic evaluations ( EE ) on adjuvant
trastuzumab therapy for early - stage
breast cancer (
EBC ) with
HER2 - positive since last search and other EEs missed warrant a more extensive review , this study aimed to systematically review EEs of adjuvant
trastuzumab compared with chemotherapy alone for
HER2 - positive
EBC . -
pubmed
Evidence shows cost - effectiveness of
trastuzumab for
HER2 - positive
EBC in HICs . -
pubmed
Therefore , to differentiate between these conditions , several dynamic tests , including those with
desmopressin (
DDAVP ) ,
corticotrophin - releasing hormone (
CRH ) , and
Dex /
CRH have been developed . -
pubmed
N - Ethylmaleimide prevents destruction of
corticotropin (
ACTH ) in plasma . -
pubmed
Addition of
N - ethylmaleimide , an inhibitor of proteolytic enzymes , to samples for
plasma corticotropin determinations prevents destruction of the hormone at room temperature for at least 72 h. A concentration of 125 mg per liter of blood is effective . -
pubmed
N - Ethylmaleimide is not so effective in preventing
corticotropin degradation in whole blood . -
pubmed
CALLA reactivity and
periodic acid Schiff ( PAS ) staining correlate poorly , with
CALLA reacting with cells from 86 % ( 64 of 74 ) of patients with PAS - positive and 76 % ( 29 of 38 ) of those with PAS - negative
non - T - cell ALL . -
pubmed
The
amino acid sequence of
bovine cardiac tamponin - C. -
pubmed
The factors related to increased fatality included
hepatic cirrhosis , a combination of
pneumonia and
meningitis , complications such as
shock ,
respiratory insufficiency ,
central nervous system disorders and
circulatory acidosis , and laboratory findings such as
thrombocytopenia , absence of
leucocytosis and increased amounts of serum
creatinine ,
aspartate aminotransferase and
alanine aminotransferase on admission to the hospital . -
pubmed
Impact of
apo E phenotype on the regulation of
cholesterol metabolism . -
pubmed
E2/2 = 1 , E2/3 = 2 , E2/4 = 3 ... ) was negatively associated with
cholesterol synthesis and fractional removal of
LDL apo B and positively with the total and
LDL cholesterol and
LDL apo B concentrations . -
pubmed
Reduction of fat and
cholesterol consumption lowered while an increase of dietary
cholesterol enhanced the
LDL cholesterol concentration proportionately to the
apo E subscript when
cholesterol absorption efficiency was reduced in proportion to fat intake . -
pubmed
Low absorption , effective elimination and high synthesis of
cholesterol associated with low synthesis and effective removal of LDL
apo B seem to be factors keeping serum
cholesterol low during different diets . -
pubmed
The findings also suggest that the
apo E phenotypes regulate
cholesterol metabolism during basal conditions and serum
cholesterol responses to dietary modifications . -
pubmed
Association of
birdshot retinochoroidopathy and
HLA - A29 antigen . -
pubmed
However , this study seemed to confirm the need for a new approach to the intensive care of brain - dead subjects during lung procurement : the time elapsed before procurement should be shortened , the amount of medicinal solutions administered should be reduced , Ringer
lactate and packed cells should be used in preference to
albumin derivatives , and arterial blood pressure , rather than diuresis , should be maintained . -
pubmed
We suggest that the
hypotensive action of
ketanserin is in part related to an interaction at
alpha 1 - adrenoceptors . -
pubmed
In contrast , contractions elicited during prolonged
hypoxia were less sensitive to
verapamil which at a concentration of 1 microM only caused a
16.2 + 2.2 % ( n 12 ) reduction of these responses . -
pubmed
The
human cytochrome Cyp1A2 gene contains regulatory elements responsive to
3 - methylcholanthrene . -
pubmed
Deletion of flanking sequence to -1079 resulted in a loss of
3 - methylcholanthrene - induced
chloramphenicol acetyltransferase activity . -
pubmed
The strongest
3 - methylcholanthrene - induced
chloramphenicol acetyltransferase activity , a 4 - fold increase , was observed for a DNA fragment located at -3202 to -1595 . -
pubmed
These results suggest that the
3 - methylcholanthrene - responsive
Cyp1A2 element may be regulated in a tissue - specific manner . -
pubmed
Degradation and activation of
parathion analogs by microsomal
enzymes . -
pubmed
Copper content in cervical mucus of women wearing plain and
copper - bearing
spring coils . -
pubmed
Interaction of
clonidine and
clonidine analogs with
human platelet alpha 2 - adrenergic receptors . -
pubmed
The effects of
clonidine and its
p - amino analogs on
platelet adenylate cyclase were also evaluated . -
pubmed
This enzyme is inhibited by
epinephrine acting via
alpha 2 - adrenergic receptors . -
pubmed
Both
clonidine and
p - aminoclonidine cause slight inhibition of basal
adenylate cyclase and reverse the inhibition induced by
epinephrine . -
pubmed
These observations indicate that
clonidine is a partial agonist for
platelet alpha 2 - adrenergic receptors . -
pubmed
If preparations of membrane fragments are incubated with
[ gamma 32P ] ATP in the absence of
type a1 protein kinase , until all membrane bound protein acceptor sites are saturated with
phosphate addition of
type a1 protein kinase causes the incorporation of more
phosphate into the membrane proteins . -
pubmed
The nature of the membrane proteins phosphorylated by
type a1 protein kinase and the intrinsic
protein kinase of the preparation was compared by
SDS gel electrophoresis . -
pubmed
Membrane preparations were phosphorylated with
[ gamma 32P ] ATP in the absence of added
type a1 protein kinase and with
[ gamma 33P ] ATP in the presence of the enzyme . -
pubmed
A comparison of the distribution of protein bound
33P and
32P made it quite clear that
type a1 protein kinase catalysed the phosphorylation of different membrane proteins from those which are phosphorylated by the intrinsic
protein kinase activity of the preparations . -
pubmed
Vasopressin - dependent and -independent control of the release of
adrenocorticotropin . -
pubmed
We tested the possibility that
vasopressin mediates the responses of
adrenocorticotropin (
ACTH ) to electrical stimulation of various areas of the hypothalamus . -
pubmed
Intraventricular administration of antiserum to
vasopressin blocked the release of
ACTH induced by electrical stimulation of the paraventricular nucleus ( PVN ) , suggesting a role for the vasopressinergic projection from PVN to the external zone of the median eminence . -
pubmed
Thus , there is at least one
corticotropin releasing factor released from nuclei other than PVN that is distinct from
vasopressin . -
pubmed
The cleavage of
insulin by the partially purified
insulin protease was studied using the four
[ 125I ] tyrosine - monoiodoinsulins (
tyrosine A-14 and A-19 of the A - chain ;
tyrosine B-16 and B-26 of the B - chain ) . -
pubmed
Thus , the
insulin protease appears to first degrade
insulin to multiple products with molecular sizes slightly smaller than
insulin and subsequently to small peptides ( e.g containing
tyrosine A-19 ) and
amino acids ( e.g. -
pubmed
To assess the contribution of serum
total proteins ,
albumin ,
cholesterol and
immunoglobulin levels to low anion gap , a coefficient of correlation was calculated . -
pubmed
The low anion gap correlated with low serum
total proteins ( less than 4 g / dl ) and
serum albumin levels ( less than 1.5 g / dl ) but not to serum
cholesterol or
immunoglobulin levels . -
pubmed
An increase in
ferritin concentration in serum was concurrent with decreased
iron concentration . -
pubmed
On the day the ovulatory dose of
hCG was given , as determined by the first urinary
estrogen value greater than 50 microgram/24 hours ,
estrogen excretion was similar whether single or multiple follicles of potential ovulatory size were present , but mean follicular diameters were significantly smaller when there was more than 1 follicle . -
pubmed
Although ultrasound scan does not supplant
estrogen monitoring , it can assist in the early detection of
hyperstimulation , in the more accurate timing of the ovulatory dose of
hCG , and in the withholding of
hCG when 3 or more ovulations are possible . -
pubmed
The
formalin - fixed and paraffin - embedded buccal pouch sections were stained immunohistochemically with use of monoclonal antibodies for
cytokeratins . -
pubmed
Since the terminal
carbohydrate moieties of some but not all of the plaques , which mainly consist of
beta A4 protein , are identical to those of the microglial cells it is concluded that glycoproteins within these plaques have been synthesised by the microglial cell . -
pubmed
As part of a program to prepare
delta 8 ( 14 )- 15 - ketosterols that can not readily be metabolized to
cholesterol or side - chain oxygenated species , we have prepared
3 beta - hydroxy-7 alpha - fluoro-5 alpha - cholest-8 ( 14 )- en-15 - one ( VII ) and the
9 alpha - hydroxy (
IV ) ,
9 alpha - fluoro ( VI ) and
7 alpha - fluoro ( VIII ) derivatives of
3 beta - hydroxy-25,26,26,26,27,27,27 - heptafluoro-5 alpha - cholest-8 ( 14 )- en-15 - one (
II ) . -
pubmed
Sterol IV was prepared by oxidation of the
delta 8,14 dienol ethyl ether of the
3 beta - acetate of
II with
m - chloroperbenzoic acid , followed by mild alkaline hydrolysis of the
3 beta - acetate derivative of
IV . -
pubmed
Treatment of
IV with
hydrogen fluoride - pyridine gave VI . -
pubmed
Chemical , biochemical , pharmacokinetic , and biological properties of
L-680,833 : a potent , orally active
monocyclic beta - lactam inhibitor of
human polymorphonuclear leukocyte elastase . -
pubmed
A series of potent and highly selective time - dependent
monocyclic beta - lactam inhibitors of
human polymorphonuclear leukocyte elastase (
PMNE ,
EC 3.4.21.37 ) is described . -
pubmed
The intrinsic potency of these compounds , as exemplified by
L-680,833 ( k(inactivation)/K(i ) of 622,000 M-1.s-1 ) , is reflected at the cellular level where it inhibits generation of the specific N - terminal cleavage product A alpha-(1 - 21 ) from the A
alpha chain of
fibrinogen by enzyme released from isolated polymorphonuclear leukocytes stimulated with
fMet - Leu - Phe with an IC50 of 0.06 microM. -
pubmed
The inhibitory activity of
L-680,833 is also apparent in whole blood stimulated with
A23187 , where it inhibits formation of
A alpha -( 1 - 21 ) and
PMNE - alpha 1 - proteinase inhibitor complex formation with IC50 values of 9 microM. -
pubmed
The properties of
L-680,833 allow it to effectively supplement the activity of natural inhibitors of
PMNE in vivo , suggesting that this type of low - molecular - weight synthetic inhibitor could have therapeutic value in diseases where PMNE damages tissue . -
pubmed
Moreover , a bivariate distribution of cells stained for DNA and
cyclin B1 showed that , following
melphalan and
hyperthermia treatment , the fraction of
cyclin B1 - expressing cells paralleled the fraction of G2+M phase cells , thus indicating that the inability of cells to enter mitosis was not ascribable to a reduction of
cyclin B1 expression . -
pubmed
After
PDGF BB treatment , a 100 - kDa
tyrosine phosphorylated species was detected in anti -
Stat6 immunoprecipitates . -
pubmed
Cycloheximide had little effect on
Stat6 tyrosine phosphorylation . -
pubmed
In addition to
Stat6 ,
Stat5a , and
Stat5b ,
PDGF BB also induced
Jak1 tyrosine phosphorylation suggesting a potential pathway for
Stat activation . -
pubmed
Strikingly , the concurrent addition of
IL-4 enhanced
PDGF BB - induced
Iepsilon binding activity ,
Jak1 tyrosine phosphorylation , and
[ 3H ] thymidine incorporation . -
pubmed
Heteromeric channel formation and
Ca ( 2 +)-free media reduce the toxic effect of the weaver
Kir 3.2 allele . -
pubmed
We also demonstrate that the cell
death induced by weaver expression may be prevented by elimination of
calcium from the extracellular solution as well as by coexpression with the wild - type
Kir 3.2 allele , or other members of the
Kir 3.0 subfamily . -
pubmed
These results suggest that the weaver defect in
Kir 3.2 may cause cerebellar cell
death by cell swelling and
calcium overload . -
pubmed
Myocardial
beta - adrenoceptor density and plasma
catecholamines in
syndrome X. -
pubmed
We performed positron emission tomography with
( 11 ) C - CGP-12177 to measure
beta - adrenoceptor density . -
pubmed
The
C2 domain , also encoded by two exons , has an extra pair of
cysteines . -
pubmed
Inhibitory effects of anions and active site
amino acid sequence of
chicken liver L-2 - hydroxyacid oxidase A , a member of the
FMN - dependent alpha - hydroxyacid oxidizing enzyme family . -
pubmed
Monocarboxylic acids with aliphatic chains were found to be mixed inhibitors of
chicken liver L-2 - hydroxyacid oxidase A when
L-2 - hydroxy-4 - methylthiobutanoic acid was used as the substrate . -
pubmed
The finding that the binding affinity of the
enzyme for
monocarboxylic acids was directly proportional to the number of
carbon atoms in the chain strongly suggests that in addition to the electrostatic interaction due to the
carboxyl moiety , hydrophobic forces may also be involved in the binding affinity of
monocarboxylic acids to the
enzyme 's active site . -
pubmed
Oxalate , a
dicarboxylic acid , also resulted in a mixed - type inhibition of
chicken liver L-2 - hydroxyacid oxidase A , and , surprisingly , its binding affinity to the
enzyme was found to be quite high as compared with
monocarboxylic acids . -
pubmed
Oxamate was found however to be a novel type of potent inhibitor of the
enzyme . -
pubmed
Blockage of mitotic apparatus formation by
nocodazole also blocks the concentration of
hsp70 molecules observed in nontreated eggs . -
pubmed
Second , the percentage of
CB- ,
CR- , and
PV - ir neurons receiving multiple contacts on their
somata and proximal dendrites from other
calcium - binding protein neurons varied between 22 % and 85 % . -
pubmed
Identification of an essential
tyrosine residue in
nitroalkane oxidase by modification with
tetranitromethane . -
pubmed
The
enzyme is irreversibly inactivated by incubation with
tetranitromethane , a
tyrosine - directed reagent , at pH 7.3 . -
pubmed
A saturation kinetic pattern is observed when the rate of inactivation is determined versus the concentration of
tetranitromethane , indicating that a reversible
enzyme - inhibitor complex is formed before irreversible inactivation occurs . -
pubmed
The competitive inhibitor
valerate protects the
enzyme from inactivation by
tetranitromethane , suggesting an active - site - directed inactivation . -
pubmed
The UV - visible absorbance spectrum of the inactivated
enzyme is perturbed with respect to that of the native
enzyme , suggesting that treatment with
tetranitromethane resulted in nitration of the
enzyme . -
pubmed
Comparison of tryptic maps of
nitroalkane oxidase treated with
tetranitromethane in the presence and absence of
valerate shows a single peptide differentially labeled in the inactivated
enzyme . -
pubmed
After a confluency of 70 - 80 % was reached , cell proliferation was suppressed by
arginine - deficient medium (
ADM ) , and UDS was assessed autoradiographically by
3H - thymidine incorporation upon exposure to
OTA ( 10 - 2000 nM ) ,
ethyl methanesulfonate (
EMS , 5 mM , positive control ) , or
dimethyl sulfoxide (
DMSO , 0.2 % , solvent control ) . -
pubmed
In two
HUC cultures derived from adults , a significant induction of
UDS was observed in the concentration range of 50 - 500 nM
OTA . -
pubmed
The highest fraction of cells in repair (
CIR ) was found at 50 nM
OTA for the
HUC from the older male ( 50 %
CIR ) . -
pubmed
Liver hyperplasia and paradoxical regulation of glycogen metabolism and
glucose - sensitive gene expression in
GLUT2 - null hepatocytes . -
pubmed
We investigated the impact of
GLUT2 gene inactivation on the regulation of hepatic
glucose metabolism during the fed to fast transition . -
pubmed
This was correlated with
glucose-6 - phosphate (
G6P ) levels , which remained at the fed values , indicating an important allosteric stimulation of
glycogen synthase by
G6P. -
pubmed
These
G6P levels were also accompanied by a paradoxical elevation of the mRNAs for
L - pyruvate kinase . -
pubmed
Together , these data indicate that in the absence of
GLUT2 , the
G6P levels can not decrease during a fasting period . -
pubmed
Therefore , in fasting ,
GLUT2 is not required for quantitative normal
glucose output but is necessary to equilibrate cytosolic
glucose with the extracellular space . -
pubmed
By examining the subcellular localization of
GluRdelta2 mutants with various deletions in the
carboxyl - terminal cytoplasmic region , we identified J segment adjacent to transmembrane segment M4 as a key region for the efficient targeting of
GluRdelta2 to the plasma membrane . -
pubmed
Our results suggest that the juxtamembrane segment and the PDZ domain target site at the
carboxyl terminus of
GluRdelta2 play differential roles in plasma membrane targeting , synapse localization and signal transduction . -
pubmed
In three isolates , a mixture of resistant and susceptible subpopulations ( heteroresistance ) prohibited the detection of
rpoB mutations in the initial analysis ; however , in these isolates , cluster I mutations could be verified after a passage on
RIF - containing medium . -
pubmed
The serum total
triglyceride level was ( mean + /-
SE ) 188.85 + /- 5.91 mg / dl at entry , and was also significantly decreased to 158.57 + /- 4.48 mg / dl at day 90 , this reduction was found statistically significant ( P & lt ; 0.01 ) . -
pubmed
Whereas
very - low density lipoprotein cholesterol reduced significantly from 33.08 + /- 1.09 mg / dl at day 0 to 31.02 + /- 0.95 mg / dl at day 90 ( P & lt ; 0.01 ) . -
pubmed
Acarbose had no significant effect on serum total
cholesterol ,
low - density lipoprotein cholesterol , and
High - density lipoprotein cholesterol concentrations . -
pubmed
Unlike
Bcl-2 , however , the C - terminal membrane anchor region is preceded by a unique 250
amino acid insertion containing two tandem repeats . -
pubmed
The
AMPA - specific antagonist ,
GYKI 53655 , prevented a
kainate - induced increase in
ERG a- and d - waves , but was without effect on an increase in the b - wave . -
pubmed
( 2 ) The
kainate - evoked increase in
ERG a- , b- , and d - waves probably results , in part , from an excitotoxic swelling of inner retinal processes . -
pubmed
Sodium hypochlorite (
NaOCl ) addition was applied by most facilities as the temporary
post - polishment step to comply with the color discharge standard . -
pubmed
Clinic records were used to obtain patient characteristics , as well as independent variables including
CD4 + count , coexisting
hepatitis C and current
alcohol use . -
pubmed
A thyroid profile performed when he was admitted to our hospital showed several marked abnormalities :
serum thyroid stimulating hormone (
TSH ) was elevated ( 69.4 microIU / ml : normal , 0.4 - 4.2 mIU / ml ) , free
thyroxine level ( T4 ) ( 0.01 ng / dl ; normal , 0.70 - 1.60 ng / dl ) and free
triiodothyronine level ( Ts ) ( 0.60 pg / ml ; normal , 2.3 - 4.1 pg / ml ) were low . -
pubmed
However , pretreatment with
N ( ) - nitro - L - arginine - methyl ester (
L - NAME ) , an inhibitor of
nitric oxide ( NO ) synthase , significantly attenuated the inhibitory effect of E(2 ) . -
pubmed
These results suggest that E(2 ) may inhibit spontaneous electrical activity of RVLM neurons , an effect which is mediated by the activation of
NOS with the resultant of
NO release via nongenomic actions . -
pubmed
The full length ORF encodes a putative phosphoprotein containing a
serine stretch and a bipartite nuclear localization signal , which we call
Srsp1 ( "
serin - rich spermatogenic protein 1 " ) . -
pubmed
Development of an " electroptode " immunosensor :
indium tin oxide - coated optical fiber tips conjugated with an electropolymerized thin film with conjugated
cholera toxin B subunit . -
pubmed
Avidin -
biotin interactions were used to modify the fiber optics with
biotin - conjugated
cholera toxin B subunit molecules , for the construction of an immunosensor to detect
cholera antitoxin antibodies . -
pubmed
Identification of two
histidines necessary for
reovirus mRNA guanylyltransferase activity . -
pubmed
The positive effect of low pH was detected for both autoguanylylation and
GMP transfer , the two steps in the
guanylyltransferase reaction . -
pubmed
The effect of pH on enzymatic activity suggested that
histidine protonation is responsible for the observed increase in
guanylyltransferase activity . -
pubmed
Mutagenesis of the two
histidines conserved among the orthoreovirus and aquareovirus
guanylyltransferases demonstrated that they are necessary for activity . -
pubmed
The
LNG - IUS down - regulates endometrial
progesterone and
estrogen receptors and this may play a role in the mechanism responsible for BTB . -
pubmed
LNG is an androgenic
progestogen and so we examined the regulation of the
androgen receptor (
AR ) in endometrium exposed to intrauterine
LNG . -
pubmed
Furthermore , as the enzyme
17beta - hydroxysteroid dehydrogenase type 2 (
17betaHSD2 ) regulates intracellular levels of
estrogens ,
progestins and
androgens , we evaluated the changes in expression of
17betaHSD2 in the same tissue endometrial samples . -
pubmed
RESULTS : Immunohistochemistry showed that
AR and
17betaHSD2 , which were immunolocalized to the stroma and glands of endometrium respectively , were both suppressed by
LNG - IUS treatment , though moderate staining of
17betaHSD2 was evident 1 month after insertion of the
LNG - IUS . -
pubmed
AR mRNA expression was down - regulated in
LNG - exposed endometrium when compared with the proliferative phase of the menstrual cycle . -
pubmed
17betaHSD2 mRNA was significantly increased 3 months ( but not 6 - 12 months ) after
LNG - IUS insertion . -
pubmed
CONCLUSIONS : Endometrial intracellular
estradiol levels would have been suppressed by
17betaHSD2 during the first few , but not the later , months of
LNG - IUS action , and the lowered endometrial
estradiol level may contribute to the frequent BTB evident in the early months of
LNG - IUS use . -
pubmed
The subsequent decline in
17betaHSD2 would lead to elevated local intracellular
estradiol in the later months , when the BTB tends to subside . -
pubmed
The suppression of
AR by the
LNG - IUS may also play a role in BTB , as elevated
AR has been associated with
amenorrhoea . -
pubmed
Ribonucleotide reductase subunit M1 (
RRM1 ) is involved in DNA synthesis and repair and in
gemcitabine metabolism , and the
excision repair cross - complementing group 1 (
ERCC1 ) gene has been related to
cisplatin activity . -
pubmed
Thioacetamide - induced liver regeneration involves the expression of
cyclooxygenase 2 and
nitric oxide synthase 2 in hepatocytes . -
pubmed
Analysis of
cyclin D1 , E and
PCNA correlated with hepatocytes entering into the S phase of cell cycle by the effect of
TAM . -
pubmed
However , the absence of
NO delays hepatocyte regeneration , whereas
COX-2 - inhibition appears to decrease
liver damage . -
pubmed
Effects of
idoxifene on antioxidant enzyme levels of
copper ,
zinc - dependent superoxide dismutase (
CuZn - SOD ) , and cellular
glutathione peroxidase (
GSHPx ) were measured by ELISA . -
pubmed
A treatment of 0.4 mg x kg(-1 ) x d(-1 ) of
idoxifene reduced the protein levels of
collagen in the
DMN model by 41.19 % ( P<0.05 ) . -
pubmed
Protein level of
CuZn - SOD and activitiy of
GSHPx in liver treated with
DMN plus 0.4 mg / kg / d of
idoxifene were 2.65 times ( P<0.05 ) and 2.08 times greater ( P<0.05 ) than that of liver treated with
DMN alone respectively . -
pubmed
We have shown that
nocturnin acts as a
deadenylase in vitro , removing the poly(A ) tail from a mature messenger RNA in a process that either leads to degradation or translational silencing of a
message . -
pubmed
The endoplasmic reticulum (
ER ) is a fundamental organelle required for protein assembly , lipid biosynthesis , and vesicular traffic , as well as
calcium storage and the controlled release of
calcium from the
ER lumen into the cytosol . -
pubmed
The increased expression of
eNOS in dura mater and
eNOS and
nNOS in pia mater seen after stress could not be antagonized by treatment with the
migraine drug
sumatriptan . -
pubmed
The effect of mold temperature on castability of
CP Ti and
Ti-6Al-4V castings into
phosphate bonded investment materials . -
pubmed
OBJECTIVE : The aim of this study was to evaluate the castability of
CP titanium and
Ti-6Al-4V alloy castings into Rematitan Plus investment at three different mold temperatures . -
pubmed
RESULTS : The
Ti-6Al-4V alloy ( 60.86 % ) presented a better castability index than
CP Ti ( 48.44 % ) ( p & lt ; 0.000001 ) . -
pubmed
For
CP Ti , the temperature of 530 degrees C ( 23.96 % ) presented better castability than at other temperatures , 480 degrees C ( 14.66 % ) and 430 degrees C ( 12.54 % ) , with no difference between them ( p & lt ; 0.001 ) . -
pubmed
SIGNIFICANCE : Within the limitations of this study , the increase in the mold temperature of the
Rematitan Plus investment resulted in a better castability index for both materials , and
Ti-6Al-4V presented a better castability index than
CP Ti . -
pubmed
Vasopressors : 1 mg
epinephrine every 3 - 5 min
IV . -
pubmed
Epinephrine 10 microg / kg
IV or intraosseously , or 100 microg ( endobronchially ) every 3 - 5 min . -
pubmed
In parallel to
pain therapy ,
aspirin ( 160 - 325 mg PO or
IV ) and in addition
clopidogrel ( 300 mg PO ) . -
pubmed
As
antithrombin ,
heparin ( 60 IU / kg , max . -
pubmed
After
hemorrhage exclusion , thrombolysis within 3 h of symptom onset ( 0.9 mg / kg
rt - PA IV ; max 90 mg within 60 min , 10 % of the entire dosage as initial bolus , no
aspirin , no
heparin within the first 24 h ) . -
pubmed
Using this in vitro assembly model , it was found that at a molar ratio of 1:1 ;
LDS47 :
EtxB , 50 % of reassembly was inhibited , and that this inhibition increased to 90 % at a ratio of 2:1 . -
pubmed
Perfusion with the
GABA ( A ) receptor antagonist
picrotoxin ( 100 microM ) increased
KCl - evoked effect , whereas
NMDA and non -
NMDA glutamate receptor antagonists (
D - AP5 100 microM and
CNQX 30 microM , respectively ) were able to decrease
KCl - evoked effect only in the presence of
picrotoxin ( 100 microM ) . -
pubmed
Bath application of
WIN 55212 - 2 ( 10 microM ) inhibited
KCl - evoked effect ; this inhibition was blocked by the
CB ( 1 ) receptor antagonist
AM 251 ( 1 microM ) . -
pubmed
In conclusion , GABAergic and glutamatergic components are both involved in
KCl - evoked excitation of LC neurons , although
CB ( 1 ) receptors only seem to inhibit the glutamatergic component of
KCl effect in the locus coeruleus . -
pubmed
RT - PCR and immunoblotting experiments showed that almost all the
PDE4D isoforms , known as the main
cAMP - regulated
rolipram - sensitive
PDE in Sertoli cells , were expressed throughout the early postpartum period , whereas only the short
PDE4D isoforms (
PDE4D1 and
PDE4D2 ) were transcriptionally regulated by
FSH . -
pubmed
Resensitization of the
cAMP response to
FSH in 20 - day - old Sertoli cells was also associated with the highest
FSH - induced transient increase in both soluble and particulate
PDE4 activities , which suggests developmental changes in the
PKA - mediated upregulation of the catalytic activities of long
PDE4D. -
pubmed
Nineteen well characterised cases were further submitted to immunohistochemical studies for expression of
YF antigen ,
hepatitis B surface antigen (
HBsAg ) and delta virus antigen . -
pubmed
This study focused on the behavior of
topoisomerase IIalpha in response to oxidative stress induced by
hydrogen peroxide (
H ( 2 ) O ( 2 ) ) . -
pubmed
In whole cell voltage
clamp , activation of
PKC by application of
1 - oleoyl-2 - acetyl - sn - glycerol (
OAG , 10 - 30 microM ) resulted in 1 ) a reduction of I(T ) amplitude by 33 % accompanied an increase in half - width and a decrease in the maximal rise and decay rates of the I(T ) ; 2 ) a reduction of PIC amplitude by 49 % , with a depolarization of PIC onset by 4.5 mV. -
pubmed
Inclusion of
PKCI19 - 36 ( 10 - 30 microM ) , a
PKC inhibitor , in the recording pipette could block the
OAG effects on I(T ) and PIC . -
pubmed
The ability of
serotonin to hyperpolarize Vth was not altered by
PKC activation or inhibition . -
pubmed
Nylon-6 capillary - channeled polymer ( C -
CP ) fibers as a hydrophobic interaction chromatography stationary phase for the separation of
proteins . -
pubmed
Nylon-6 capillary - channeled polymer ( C -
CP ) fibers are used as the stationary phase for the hydrophobic interaction chromatography ( HIC ) separation of a synthetic protein mixture composed of
ribonuclease A ,
lysozyme , and
holotransferrin . -
pubmed
In conclusion , voluntary wheel running failed to modify sensitivity to cardiovascular action of
oxytocin but resulted in increased gene expression of
PNMT in left , but not right , heart atrium in a running activity - dependent manner . -
pubmed
Drugs that slow conduction through the AV node ( such as
digoxin , beta blockers and
calcium channel blockers ) have been used as adjuvants to therapy for the prevention of
atrial fibrillation . -
pubmed
Membrane
gamma - glutamyl transferase activity promotes
iron - dependent oxidative DNA damage in
melanoma cells . -
pubmed
A number of recent observations have suggested a potential role for membrane - bound
gamma - glutamyltransferase (
GGT ) in
tumor progression and appearance of more aggressive and resistant phenotypes , through redox interactions leading to production of reactive
oxygen species . -
pubmed
Our results indicate that higher levels of
GGT activity are associated with higher levels of background DNA damage and
oxidized bases . -
pubmed
GGT - over - expressing cells also presented with a markedly higher
glucose uptake , a phenomenon potentially leading to higher metabolic rate and oxidative DNA damage . -
pubmed
Anyway , when
GGT - over - expressing cells were incubated in the presence of
GGT substrates and a source of catalytic
iron , increased levels of DNA damage and
oxidized bases were observed , an effect completely prevented in the presence of
GGT inhibitors or various antioxidants . -
pubmed
The findings reported indicate that
GGT activity is able to promote
iron - dependent DNA oxidative damage , thus potentially representing an important mechanism in initiation / progression of neoplastic transformation . -
pubmed
To investigate the influence of the different linkage groups on the final monolayer structure , organic monolayers were prepared from
1 - alkenes and
1 - alkynes with chain lengths from
C ( 12 ) to
C ( 18 ) , and the final monolayer structures were studied in detail by static water contact angles measurements , ellipsometry , attenuated total reflectance infrared (
ATR - IR ) spectroscopy , and X - ray photoelectron spectroscopy ( XPS ) . -
pubmed
Penehyclidine hydrochloride alone has no effect on learning and memory abilities and the
synaptophysin expression . -
pubmed
PREDICTOR : Serum
glucose and
HbA ( 1c ) levels . -
pubmed
LIMITATIONS : Registry data ; casual serum
glucose measurements ;
HbA ( 1c ) values available for only a subset of participants . -
pubmed
CONCLUSIONS : Higher casual
glucose and
HbA ( 1c ) levels were not associated with mortality in maintenance hemodialysis patients with or without
diabetes . -
pubmed
Based on their
surface markers ,
cytokine production , and ability to prime naive allogenic T cells , we found that purified
BDCA-2(+)
BDCA-4(+ ) pDCs consist of at least two separate populations , which differed in their response to
oligodeoxynucleotides and
IFNs (
IFN - beta ) , and differently induced
IL-17- or
IL-10 - producing T cells . -
pubmed
Retrospective , observation study : Quantitative and qualitative effect of
ezetimibe and
HMG - CoA reductase inhibitors on LDL -
cholesterol : are there disappearance thresholds for small , dense
LDL and IDL ? -
pubmed
The correlation was between
LDL - C and small , dense
LDL as well as
mid - band lipoprotein cholesterol . -
pubmed
BACKGROUND :
EphrinA5 is one of the best - studied members of the Eph - ephrin family of guidance
molecules , known to be involved in brain developmental processes . -
pubmed
Considering that this membrane - anchored
molecule may act far from the neuron
soma expressing the transcript , it is of a crucial interest to localize
ephrinA5 protein to better understand its function . -
pubmed
Plasminogen is a precursor to the fibrinolytic enzyme
plasmin and is known to undergo large conformational changes when subjected to low molecular
lysine analogues such as
tranexamic acid ( TA ) or
epsilon - amino - n - caproic acid (
EACA ) . -
pubmed
The interaction was studied by the
quartz crystal microbalance with dissipation monitoring ( QCM - D ) technique as well as by surface plasmon resonance (
SPR ) based sensing . -
pubmed
Upon binding of TA or
EACA , changes in the
plasminogen structure were recorded as distinct , although small , DeltaD responses which were used to determine affinity constants . -
pubmed
BACKGROUND /
AIM : this study evaluated esophageal radioprotection by the
Gramicidin S (
GS ) derived -
nitroxide ,
JP4 - 039 , a mitochondrial targeting peptide - isostere covalently - linked to
4 - amino - Tempo , delivered in a novel swallowed oil - based ( F15 ) formulation . -
pubmed
RESULTS :
JP4 - 039 / F15 or
MnSOD - PL increased survival compared to irradiated controls ( p<0.0001 for either ) . -
pubmed
Among the genes altered in expression level in
AD brains was a transcript encoding a novel protein ,
SDIM1 , that contains 146
amino acids , including a typical signal peptide and two transmembrane domains . -
pubmed
HLA class I antigens were typed serologically using microlymphocytotoxicity technique . -
pubmed
Under the optimized conditions [ ECL detection at 1.15 V , 25 mmol / L
phosphate buffer solution (
PBS ) , pH 8.0 , as running buffer , separation voltage 12.5 kV , 5 mmol / L
Ru ( bpy ) 32 + with 60 mmol / L
PBS , pH 8.5 , in the detection cell ] linear correlation ( r = 0.9987 ) between ECL intensity and
ephedrine concentration was obtained in the range 6.0 x 10 - 8 - 6.0 x 10 - 6 g / mL. -
pubmed
The number of binding sites and the binding constants between
ephedrine and
BSA ,
Cyt - C and Mb were 8.52 , 12.60 , 10.66 and 1.55 x 104 mol / L , 6.58 x 103 mol / L and 1.59 x 104 mol / L , respectively . -
pubmed
Structure and function of
glutamate receptor amino terminal domains . -
pubmed
The
amino terminal domain (
ATD ) of
ionotropic glutamate receptor (
iGluR ) subunits resides at the extracellular region distal to the membrane . -
pubmed
In this study , we generated a
caspase-1 - ESC line transfected with a
mifepristone - regulated
caspase-1 expression system . -
pubmed
Mifepristone induced
caspase-1 overexpression both in differentiated and undifferentiated
caspase-1 - ESCs . -
pubmed
All the undifferentiated
caspase-1 - ESCs were induced to
death after
mifepristone treatment . -
pubmed
Tumors derived from undifferentiated
caspase-1 - ESCs were eliminated following 3 weeks of
mifepristone treatment in vivo . -
pubmed
However , differentiated
caspase-1 - ESCs survived well under the condition of
mifepristone - induced
caspase-1 overexpression . -
pubmed
These data demonstrate that
mifepristone - induced
caspase-1 overexpression in ESCs can eliminate the potential
tumor formation meanwhile spares the differentiated cells in the host brains . -
pubmed
Reference measurement procedure for total
glycerides by isotope dilution
GC -
MS . -
pubmed
BACKGROUND : The CDC 's Lipid Standardization Program established the
chromotropic acid ( CA ) reference measurement procedure (
RMP ) as the accuracy base for standardization and metrological traceability for
triglyceride testing . -
pubmed
To overcome these problems the CDC developed an isotope dilution
GC -
MS ( ID -
GC -
MS )
RMP for total
glycerides in serum . -
pubmed
The samples were dried , derivatized with
acetic anhydride and
pyridine , extracted with
ethyl acetate , and analyzed by ID -
GC -
MS . -
pubmed
CONCLUSIONS : This ID -
GC -
MS RMP has the specificity and ruggedness to accurately quantify total
glycerides in the serum pools used in the CDC 's Lipid Standardization Program and demonstrates sufficiently acceptable agreement with the NIST primary
RMP for total
glyceride measurement . -
pubmed
Effects of
ethanol on
CYP2E1 levels and related oxidative stress using a standard balanced diet . -
pubmed
Expression of
cytochrome P4502E1 (
CYP2E1 ) is very much influenced by nutritional factors , especially
carbohydrate consumption , and various results concerning the expression of
CYP2E1 were obtained with a low -
carbohydrate diet . -
pubmed
This study describes the effects of
ethanol treatment on
CYP2E1 levels and its relationship with oxidative stress using a balanced standard diet to avoid low or high
carbohydrate consumption . -
pubmed
The results have shown that
ethanol administration was associated with
CYP2E1 induction and stabilization without related oxidative stress . -
pubmed
Our findings suggest that experimental models with a low -
carbohydrate / high - fat diet produce some undesirable
CYP2E1 changes that are not present when a balanced standard diet is given . -
pubmed
Protein
hydrogen /
deuterium exchange (
HDX ) followed by
protease digestion and mass spectrometric (
MS ) analysis is accepted as a standard method for studying protein conformation and conformational dynamics . -
pubmed
The utility of the
HDX MS platform is demonstrated by exploring the conformational transition of a monoclonal antibody by increasing concentrations of
guanidine . -
pubmed
Based on spatial interpolation data from 2003 to 2010 , combined with almost 30 years of
nitrogen and
phosphorus pollutant data , this research analyzed the variations in total
nitrogen (
TN ) ,
nitrate nitrogen (
NO ( 3 )- N ) , total
phosphorus (
TP ) and
phosphate - P (
PO ( 4 )- P ) in the Yangtze River Estuary ( YRE ) . -
pubmed
Role of the
phosphatidylinositol 3 - kinase and
extracellular signal - regulated kinase pathways in the neuroprotective effects of
cilnidipine against
hypoxia in a primary culture of cortical neurons . -
pubmed
Cilnidipine , a
calcium channel blocker , has been reported to have neuroprotective effects . -
pubmed
We investigated whether
cilnidipine could protect neurons from
hypoxia and explored the role of the
phosphatidylinositol 3 - kinase (
PI3 K ) and
extracellular signal - related kinase (
ERK ) pathways in the neuroprotective effect of
cilnidipine . -
pubmed
The viability of a primary culture of cortical neurons injured by
hypoxia , measured by
trypan blue staining and
lactate dehydrogenase (
LDH ) assay , was dramatically restored by
cilnidipine treatment . -
pubmed
Survival signaling proteins associated with the
PI3 K and
ERK pathways were significantly increased while
death signaling proteins were markedly decreased in the primary culture of cortical neurons simultaneously exposed to
cilnidipine and
hypoxia when compared with the neurons exposed only to
hypoxia . -
pubmed
These neuroprotective effects of
cilnidipine were blocked by treatment with a
PI3 K inhibitor or an
ERK inhibitor . -
pubmed
These results show that
cilnidipine protects primary cultured cortical neurons from
hypoxia by reducing free radical stress ,
calcium influx , and
death - related signaling proteins and by increasing survival - related proteins associated with the
PI3 K and
ERK pathways , and that activation of those pathways plays an important role in the neuroprotective effects of
cilnidipine against
hypoxia . -
pubmed
We showed
EcdI activates
linoleate as
linoleyl - AMP and installs it on the first thiolation domain of
EcdA. -
pubmed
We have also established through
ATP - PP(i ) exchange assay that
EcdA loads
L - ornithine in the first module . -
pubmed
A separate
hty gene cluster encodes four enzymes for de novo generation of
L - homotyrosine from
acetyl - CoA and
4 - hydroxyphenyl - pyruvate is found from the sequenced genome . -
pubmed
Deletions in the
ecdA , and
htyA genes validate their essential roles in
echinocandin B production . -
pubmed
Five predicted
iron - centered
oxygenase genes ,
ecdG ,
ecdH ,
ecdK ,
htyE , and
htyF , in the two separate
ecd and
hty clusters are likely to be the tailoring
oxygenases for maturation of the nascent
NRPS lipohexapeptidolactam product . -
pubmed
Urinary
chromium excretion in response to an
insulin challenge is not a biomarker for
chromium status . -
pubmed
Cr is known to be mobilized in the body in response to
insulin ( or
insulin release in response to a
glucose challenge ) , resulting in an increase in urinary
Cr excretion . -
pubmed
Urine samples were collected before and after
insulin and
glucose challenges ( 0 , 2 , 6 , and 12 h postinjection ) . -
pubmed
Overall , the study demonstrated that
insulin - stimulated urinary
Cr excretion can not be used as a biomarker for
Cr status . -
pubmed
The
androgen metabolite ,
5alpha - androstane-3beta,17beta - diol (
3beta - diol ) , activates the
oxytocin promoter through an
estrogen receptor - beta pathway . -
pubmed
Therefore , a possibility is that the PVN target neurons respond to a metabolite in the
testosterone catabolic pathway via an
AR - independent mechanism . -
pubmed
The
dihydrotestosterone metabolite ,
5alpha - androstane-3beta,17beta - diol (
3beta - diol ) , binds and activates
estrogen receptor - beta (
ER - beta ) , the predominant
ER in the PVN . -
pubmed
In the PVN ,
ER - beta is coexpressed with
oxytocin ( OT ) . -
pubmed
Therefore , we tested the hypothesis that
3beta - diol regulates
OT expression through
ER - beta activation . -
pubmed
In a dose - dependent manner ,
3beta - diol treatment increased OT -
luc activity when cells were cotransfected with
ER - beta , but not
ER - alpha . -
pubmed
The
3beta - diol - induced OT -
luc activity was reduced by deletion of the promoter region containing the composite hormone response element ( cHRE ) . -
pubmed
Point mutations of the cHRE also prevented OT -
luc activation by
3beta - diol . -
pubmed
These results indicate that
3beta - diol induces OT promoter activity via
ER - beta - cHRE interactions . -
pubmed
Potential prognostic factors ( pre- and
post - implant dosimetric indices ,
GS 3 + 4 versus 4 + 3 ,
androgen deprivation therapy ( ADT ) ) were analysed . -
pubmed
insulin and
glucose ) as an independent concentration ( of e.g. -
pubmed
, the largest possible decrease in
glucose predicted by the model is smaller than reported decreases , and the model predicts no decrease whatsoever in
glucose xinsulin , in contrast to large observed decreases in homeostatic model assessment
insulin resistance ( HOMA - IR ) . -
pubmed
On the other hand , both effects can be accounted for if the surgery leads to a substantial increase in some substance that opens an alternative
insulin - independent pathway for
glucose transport into muscle cells , which perhaps uses the same intracellular pool of
GLUT-4 that is employed in an established
insulin - independent pathway stimulated by muscle contraction during exercise . -
pubmed
However , the present results suggest that another possible mechanism should be added to the list of candidates : enhanced production in the lower intestine of a substance which opens an alternative
insulin - independent pathway for
glucose transport . -
pubmed
The production of
nitric oxide (
NO ) , the expression of
metalloprotease-2 (
MMP-2)/
tissue inhibitor metalloprotease-2 (
TIMP-2 ) , and the activation of
MMP-2 were measured under the different levels of cyclic equibiaxial deformation either in the presence or absence of
TNF - alpha . -
pubmed
Our study indicated that cyclic equibiaxial deformation - induced production of
NO and
MMP-2 /
TIMP-2 . -
pubmed
An N - terminal - domain (
NTD ) and adjacent catalytic body ( CB ) make up subunit - alpha of
ribonucleotide reductase (
RNR ) , the rate - limiting enzyme for de novo
dNTP biosynthesis . -
pubmed
A strong linkage exists between ligand binding at the
NTD and oligomerization - coupled
RNR inhibition , inducible by both
dATP and
nucleotide chemotherapeutics . -
pubmed
Both the
NTD and the CB of the
HE bind
dATP . -
pubmed
However , although the
NTD is the sole physical tether to support alpha2 and/or
beta2 associations in the
dATP - bound
alpha6 or
alpha4beta4 fully inhibited
RNR complexes , the binding of
dATP to the
HE NTD only partially suppresses
HE activity and fully precludes formation of higher - order
HE oligomers . -
pubmed
Efflux of
rhodamine 123 or
calcein - acetoxymethylester was used to study the
P - gp profile . -
pubmed
P - gp - overexpressing National Cancer Institute /
Adriamycin - Resistant cells were also sensitive to
B014 . -
pubmed
B014 - induced loss of
Mcl-1 was accompanied by activation of
caspases-3 , - 7 , - 8 and - 9 , and initiation of apoptosis . -
pubmed
Inspection of these residues ' spatial arrangement in the active site , allowed us to conclude that
PncC is a
serine - amidohydrolase , employing a
Ser /
Lys dyad for catalysis . -
pubmed
Analysis of the
PncC structure in complex with a modeled
NMN substrate supported our conclusion , and enabled us to propose the catalytic mechanism . -
pubmed
In adipose , the most activated pathways by SCE were
nicotinate and
nicotinamide metabolism , long - chain
fatty acid transport , oxidative phosphorylation ,
inflammation , T cell and B cell receptor signaling , and
mTOR signaling . -
pubmed
TiNT were synthesized via hydrothermal treatment of
titania powders in
NaOH solution at 130 C for 48 h. The obtained samples were characterized by UV - Vis absorption spectroscopy , Brunauer - Emmett - Teller ( BET ) surface area , XRD ,
TEM , XPS , and
TG analysis . -
pubmed
MEK is very resistant to photocatalytic degradation with the prepared materials ; , however , the results show that modification of the
TiNT with
nitrogen in a proportion of 1 to 1 (
TiNT to
urea weight ratio ) and calcination at 400 C lead to materials with high photocatalytic activity under ultraviolet radiation and moderate photocatalytic activity under solar radiation for degradation of
H2S. -
pubmed
EGFR modulates DNA synthesis and repair through
Tyr phosphorylation of histone
H4 . -
pubmed
Here , we identify a
tyrosine phosphorylation site at Y72 of histone
H4 , which facilitates recruitment of histone methyltransferases (
HMTases ) ,
SET8 and
SUV4 - 20H , to enhance its
K20 methylation , thereby promoting DNA synthesis and repair . -
pubmed
We designed an experimental , open , prospective and comparative study , divided in seven groups : 1 -
BMP-2+
calcium phosphate ( BT ) ; 2 -
BMP-2+acellular dermal matrix ( BM ) ; 3 -
BMP-2+
calcium alginate ( BA ) ; 4 - TCP ; 5 - MDM ; 6 - ALG ; 7 - Bone autograft ( BAG ) . -
pubmed
CONCLUSIONS :
Bone morphogenetic protein type 2 increased bone regeneration in experimental skull reconstruction , especially when combined to
calcium phosphate . -
pubmed
AIM : The aim of this study was to determine whether the sphenovomerine suture ( SVS ) can be used as a landmark to localize the sphenoidal
sinus ostium . -
pubmed
Based on the new model , the polarity of the electronic ground state ( S0 ) in
indoles is very similar to that of the first electronic excited state (
S1 ) producing this structured emission ; however , this excited state can lead to a highly dipolar excited state (
S1 ' ) with largely structureless emission under the influence of the polarity of the medium , and also , very likely , of its viscosity . -
pubmed
Thus , the polarized emission spectra for
indole in
glycerol at 283 K and 223 K showed the transition dipole moments for the emission from the first two excited states in a polar medium ,
S1 and
S1 ' , to differ by less than 20 . -
pubmed
Structure - activity relationship in a
purine - scaffold compound series with selectivity for the endoplasmic reticulum
Hsp90 paralog
Grp94 . -
pubmed
PCC7120 carries three genes ( all4978 , all7016 , and alr7522 ) encoding putative
heme - binding
GAF (
cGMP - specific phosphodiesterases ,
adenylyl cyclases , and
FhlA ) proteins that were annotated as transcriptional regulators . -
pubmed
The " as isolated " form of All4978 , identified by Soret band ( lambdamax = 427 nm ) , was assigned by electronic absorption ,
EPR , and resonance Raman spectroscopy as a hexa - coordinated low spin
Fe ( III ) heme with a distal
cysteine ligand ( absorption of delta - band around 360 nm ) . -
pubmed
Cys -
His ligand switches have been reported as putative signaling mechanisms in other
heme - binding proteins ; however , these novel cyanobacterial proteins are the first where such a ligand - switch mechanism has been observed in a
GAF domain . -
pubmed
In the low - dose
PEG - b - PLA - treated group , a significantly reduced number of regular estrous cycles , increased pituitary weight due to
hyperemia ,
vascular dilatation and
congestion , altered course of hypothalamic
gonadotropin - releasing hormone - stimulated
luteinizing hormone secretion , and increased
progesterone serum levels were observed . -
pubmed
Acute RV apical pacing decreased LV longitudinal
strain from
16.1 + - 3.7 % in
sinus rhythm to 14.4 + - 3.3 % ( p = 0.03 ) and LV base rotation from -8.4 + - 3.6 to -6.4 + - 4.0 ( p = 0.04 ) . -
pubmed
Daily 2 %
chlorhexidine gluconate bath
wash in a tertiary adult intensive care and high dependency units to reduce risk of hospital acquired multi resistant organisms : a best practice implementation project . -
pubmed
In an effort to combat increasing
nosocomial infections rates within the intensive care / high dependency unit setting , Canberra Hospital has implemented a daily 2 %
chlorhexidine gluconate bath
wash in combination as part of a best practice policy to reduce hospital acquired multi resistant organism rates of colonization . -
pubmed
METHODS : The project used the Joanna Briggs Institute 's Practical Application of Clinical Evidence System and Getting Research into Practice audit tools for promoting change in 2 %
chlorhexidine gluconate wash health practice . -
pubmed
CONCLUSIONS : The project was successful in increasing knowledge surrounding 2 %
chlorhexidine gluconate wash administration and has provided a future direction for sustaining evidence - based practice change . -
pubmed
Prophylactic measures in the form of
GnRH antagonist ,
cabergolin and plasma expanders were given after oocyte retrieval . -
pubmed
Scanning tunneling microscopy (
STM ) measurements reveal areas with reconstruction lines resulting from uniaxial
strain relief due to the lattice mismatch of
Fe and
Ir atoms , as well as pseudomorphic strained areas . -
pubmed
Magnetic field - dependent spin - polarized
STM measurements of the reconstructed
Fe double layer reveal cycloidal spin spirals with a period on the nm scale . -
pubmed
We investigated the effects of
PVA concentration ( X1 ) and solvent to antisolvent ratio ( X2 ) on the quality attributes like mean particle size (
Y1 ) ; % drug content ( Y2 ) ; and time required to 90 % drug release (
Y3 ) via 3(2 ) factorial design . -
pubmed
A significant decrease in the expression of
CD54 ,
CD80 ,
CD86 , and
HLA - DR membrane antigens versus controls was observed after 24 hours of treatment with the different
clodronate doses assayed ( P & lt ; .05 ) . -
pubmed
A significant decrease ( P = .004 ) in
ALP activity was found after 24 hours of treatment with the lowest dose ( 10(-9 ) mol / L ) , and a significant decrease in
calcium deposition was found after 15 and 21 days of treatment ( P & lt ; .05 ) . -
pubmed
Phosphatase
Rtr1 Regulates Global Levels of
Serine 5
RNA Polymerase II C - Terminal Domain Phosphorylation and Cotranscriptional Histone Methylation . -
pubmed
Rtr1 is a conserved protein that colocalizes with
RNA polymerase II (
RNAPII ) and has been shown to be important for the transition from elongation to termination during transcription by removing
RNAPII CTD serine 5 phosphorylation ( Ser5 - P ) at a selection of target genes . -
pubmed
Clinical Accuracy Study of an
GDH -
NAD Blood
Glucose Monitoring System Using the Performance Criteria of ISO 15197:2013 . -
pubmed
Studies have shown that the
deficiency of cTnI or mutations in
cTnI ( particularly in the C - terminus of
cTnI ) results in
diastolic dysfunction ( impaired relaxation ) due to an increased myofibril sensitivity to
calcium . -
pubmed
Furthermore ,
calcium desensitizing in correction of impaired relaxation in myocardial cells due to
cTnI mutations is discussed . -
pubmed
,
adiponectin levels ,
vitamin B12 levels ) , polysomnography ( abnormalities in delta sleep ratio ) , neurochemistry ( i.e. -
pubmed
Single
nucleotide polymorphisms ( SNPs ) in
frizzled - related protein (
FRZB ) have been associated with
osteoarthritis , but their association with the risk of
skeletal fluorosis has not been reported . -
pubmed
While it is well established that centrally injected
angiotensin II (
Ang II ) has potent actions on sympathetic nervous activity ( SNA ) , it is less clear whether peripheral
Ang II can immediately stimulate SNA . -
pubmed
This study shows that
o - orsellinaldehyde presents anti - inflammatory and pro - apoptotic properties by acting as
IKK-2 inhibitor . -
pubmed
Secreted
LOXL2 promotes extracellular matrix cross - linking by catalyzing the oxidative deamination of
peptidyl lysine . -
pubmed
We discovered that upon secretion in cell culture ,
LOXL2 undergoes proteolytic processing of the first two of four scavenger receptor
cysteine - rich domains at the N - terminus . -
pubmed
Furthermore ,
DEHP exposure remarkably impaired the localization and protein level of
Juno , the
sperm receptor on the membrane of oocytes . -
pubmed
The influence of
oxytocin and
vasopressin on men 's judgments of social dominance and trustworthiness : An fMRI study of neutral
faces . -
pubmed
AVP attenuated left temporoparietal junction activity as
face ratings increased , a result consistent with
AVP influencing mentalization . -
pubmed
Ferumoxytol and
CpG oligodeoxynucleotide 2395 synergistically enhance antitumor activity of macrophages against
NSCLC with
EGFRL858R / T790 M mutation . -
pubmed
Purpose : Drug resistance is a major challenge for
epidermal growth factor receptor (
EGFR)-
tyrosine kinase inhibitors ( TKIs ) treatment of
lung cancer . -
pubmed
Ferumoxytol (
FMT ) drives macrophage ( MPhi ) transformation towards a
M1 - like phenotype and thereby inhibits
tumor growth . -
pubmed
CpG oligodeoxynucleotide 2395 ( CpG ) , a
toll - like receptor 9 (
TLR9 ) agonist , is an effective therapeutic agent to induce anticancer immune responses . -
pubmed
Results : FMT and
CpG synergistically enhanced
M1 - like gene expression in MPhi , including
tumor necrosis factor - alpha ,
interleukin ( IL )- 12 ,
IL-1alpha ,
IL-1beta ,
IL-6 and
inducible nitric oxide synthase (
iNOS ) . -
pubmed
Conclusion :
FMT acted synergistically with
CpG to activate MPhi for suppressed proliferation and promoted apoptosis of
NSCLC cells via
EGFR signaling . -
pubmed
Thus , combining FMT and
CpG is an effective strategy for the treatment of
NSCLC with
EGFRL858R / T790 M mutation . -
pubmed
, intragastric ) during this period attenuated the histological signs of
steatohepatitis and lowered the serum and liver
triglyceride contents , as well as the serum activities of
aspartate aminotransferase ,
alanine aminotransferase , and
alkaline phosphatase . -
pubmed
Betulin ( 100 mg / kg ) decreased the liver /
body weight ratio and inhibited the increase in the serum levels of
TNFalpha ,
IL-1beta ,
TGFbeta , and
hyaluronic acid , demonstrating hepatoprotective , anti - inflammatory , and antifibrotic potential . -
pubmed
In addition , the expression of
IDH3 significantly decreased in the
carbendazim - resistant mutant nt167 (
Fgbeta2 F167Y ) . -
pubmed
Furthermore , we found that
carbendazim - resistance associated
beta2 -
tubulin substitutions reducing the interaction intensity between
beta2 - tubulin and
IDH3 . -
pubmed
Interestingly , we demonstrated that
beta2 -
tubulin inhibitor
carbendazim can disrupt the interaction between
beta2 - tubulin and
IDH3 . -
pubmed
Estrogen and
progesterone receptor (
ER ,
PR ) positivity was more common ( 47.6 % and 28.2 % , respectively ) in endometrioid
carcinomas as compared with serous . -
pubmed
The aim of this study was to determine the effect of
DON , administered alone or with bentonite , on the histological structure of the skin and the content of
collagen and
elastin . -
pubmed
A dose of 3.7 mg / kg
DON in feed without or with 0.05 % bentonite causes the absence of immature
collagen in the dermis , but the addition of 0.2 %
bentonite in the feed reveals the presence of immature
collagen and increase the percentage of the
elastin . -
pubmed
cGAS /
STING - dependent apoptotic effects are required for
paclitaxel response in vivo , and they are amplified by sequential , but not synchronous , administration of BH3 mimetics . -
pubmed
SDA ( 50 muM ) decreased both production and cell - surface expression of
intercellular adhesion molecule ( ICAM )- 1 ; however
EPA and
DHA resulted in greater reduction of
ICAM-1 production and expression . -
pubmed
EPA and
DHA also significantly lowered production of
monocyte chemoattractant protein 1 ,
interleukin ( IL )- 6 and
IL-8 . -
pubmed
DHA significantly decreased nuclear factor kappa - light - chain - enhancer of activated B cells (
NFkappaB)p105 gene expression and phosphorylated
NFkappaBp65 protein . -
pubmed
Both
EPA and
DHA ( 50 muM ) significantly decreased
cyclooxygenase ( COX )- 2 protein . -
pubmed
Nanoformulated
quinacrine regulates
NECTIN-4 domain specific functions in
cervical cancer stem cells . -
pubmed
were used to delineate the function of each domain of
NECTIN-4 in
cancer and their regulation by nano - formulated
quinacrine (
NQC ) . -
pubmed
NQC down - regulated these phenomena by modulating the endo - domain and ecto - domain of
NECTIN-4 . -
pubmed
Thus , current study suggested that endo - domain of
NECTIN-4 translocated into nucleus and increased the DNA repair and ecto - domain of
NECTIN-4 enhanced the angiogenesis , whereas
NQC inhibits these processes . -
pubmed
The effect of EEG on macrophage
cholesterol efflux and
ABCA1 /
G1 expression was observed under silenced
LXRalpha expression . -
pubmed
RESULTS : EEG reduced serum
cholesterol ( 20.33 + - 3.62 mmol / L vs 34.56 + - 8.27 mmol / L for the model group ) and
LDL - C , reduced the area of arterial plaques ( 24.8 + - 10 % vs 53.9 + - 15.2 % for the model group ) and Intima / Medium thickness ratio , increased faecal
bile acid content , upregulated
LXRalpha ,
CYP7A1 ,
ABCA1 / G1 ,
ABCG5 /
G8 expression in the liver , small intestine and macrophages . -
pubmed
After silencing
LXRalpha in macrophages , the ability of EEG to promote
cholesterol efflux was inhibited . -
pubmed
DISCUSSION AND CONCLUSION :
EEG exert lipid - lowering and anti - atherosclerotic effects via upregulating expression of
LXRalpha and downstream genes associated with reverse
cholesterol transport and metabolism . -
pubmed
This protocol is for isolating total RNA from either larvae (
L1 -
L4 ) or adult worms using a monophasic lysis
reagent . -
pubmed
More importantly ,
chidamide was proved effective in
MM by targeting
SDHA , and expression of
SDHA was increased by
chidamide through acetylating H3K27 site of
SDHA . -
pubmed
The structures of the new compounds were established as
3 - O - beta - D - galactopyranosyl ( 20S , 24 R )- 3beta,16beta,25,29 - tetrahydroxy-20,24 - epoxycycloartane-29 - O - beta - D - glucopyranoside ( 1 ) ,
3 - O - beta - D - glucopyranosyl -( 1 2 )- alpha - arabinopyranosyl-3beta,22xi,30 - trihydroxycycloart-24 - en-21 - oic acid alpha - L - arabinopyranosyl -( 1 6 )- beta - D - glucopyranoside ( 2 ) and
3 - O -[ alpha - L - rhamnopyranosyl -( 1 3 )- beta - D - xylopyranosyl -( 1 3 )- alpha - L - rhamnopyranosyl -( 1 2 )- alpha - L - arabinopyranosyl ]- oleanolic acid 28 - O - beta - D - glucopyranosyl ester ( 3 ) on the basis of extensive NMR and HR - ESI -
MS analyses , along with acid hydrolysis . -
pubmed
Besides , supplementation of
PQQ Na2 within diet decreased ( P < 0.05 ) the mRNA expressions of
IL-1beta and
IL-10 in the duodenal mucosa of birds on day 20 . -
pubmed
However , birds fed with
PQQ Na2 showed higher ( P < 0.05 )
GSH - Px activity in serum and higher ( P < 0.05 )
T - SOD activities in liver on day 21 and day 42 . -
pubmed
The 22
tRNA genes ranged from 65 bp ( tRNA -
Cys ) to 75 bp ( tRNA - Leu1 ) . -
pubmed
The chain - extension of
BCP seeds by an amorphous
coil - type polymer within the seed core composed of semifluorinated liquid - crystalline blocks triggers the deforming , stretching , and directional growth of the seeds along the long axis , eventually leads to nanorods . -
pubmed
METHODS : six samples of PM10 , PM2.5 and
PM1 were collected between the May 4th and 22nd 2020 in Madrid , on
quartz fiber filters by using MCV high volume samplers ( 30 m3 h-1 flow ) with three inlets ( Digitel DHA-80 ) for sampling PM10 , PM2.5 and
PM1 . -
pubmed
These results suggest that the lower accumulation of glycogen in the muscle after repeated administrations of
glucocorticoid may be primarily due to an abnormality in the
glycogen synthetase system . -
pubmed
In this particular osmotic regulation , phosphorylation of
OmpR at an
aspartate residue (
Asp-55 ) by
EnvZ plays an important role . -
pubmed
The previously isolated mutant ,
ompR55Q , with the
amino acid replacement of
Asp-55 to
Gln , exhibits an
OmpF- and
OmpC- phenotype . -
pubmed
The intragenic suppressor mutation we isolated results in the
amino acid replacement of
Tyr-102 to
Cys in the N - terminal domain of
OmpR , and exhibits an
OmpF+ and
OmpC+ phenotype in response to the medium osmolarity in an
EnvZ - independent manner . -
pubmed
It was revealed that this
amino acid replacement in
OmpR enhances the in vitro DNA - binding ability to the cognate DNAs . -
pubmed
Responses to exogenously applied
glycine and
GABA were recorded using the whole - cell voltage
clamp method ( pipette
solution contained 121 mM -
Cl- ) . -
pubmed
Because glycosaminoglycans and particularly hyaluronan (
hyaluronic acid , HA ) , may play an important role in the extracellular matrix response to
early injury and subsequent
fibrosis , this study was undertaken to define the early time course of changes in HA and
hyaluronidase . -
pubmed
The specific activity of lysosomal
hyaluronidase was the same in
bleomycin - treated lungs and control lungs . -
pubmed
The
Ca channel blockers
nifedipine ( 30 microM ) and
verapamil ( 10 microM ) abolished the increase in
Ca uptake produced by
1,25 - dihydroxyvitamin D3 . -
pubmed
These results further support a direct action of
1,25 - dihydroxyvitamin D3 in the regulation of cardiac muscle
Ca metabolism which may involve activation of
Ca channels . -
pubmed
Isoenzyme 2 was the predominant elevated isoenzyme of
LDH , suggesting that the source of
LDH is either
hemolysis of RBCs , which occurs during freezing and thawing of bone marrow in the bags and in vivo following infusion of residual
dimethyl sulfoxide . -
pubmed
Beta - endorphin C - terminal peptide evokes
arachidonic acid release from cortical neurones . -
pubmed
Gly - Gln but not the related
dipeptide ,
glycyl - glutamic acid , caused a 2 - fold elevation of AAM release which was blocked in the absence of extracellular
calcium , in the presence of 5 mM
magnesium and by the
phospholipase A2 (
PLA2 ) inhibitor ,
mepacrine . -
pubmed
The response to
Gly - Gln was unaffected by
D - amino-2 - phospho-5 - valeric acid (
AP5 ) and
7 - chlorokynurenic acid (
7 - ClKY ) , antagonists respectively at the ligand and allosteric
glycine binding sites of the
NMDA glutamate receptor subtype . -
pubmed
The results suggest that
Gly - Gln causes AAM release by activating
PLA2 through the mediation of a
PCP / sigma - like receptor . -
pubmed
Glucose reduces
PDGF production and cell proliferation of cultured vascular endothelial cells . -
pubmed
Both
PDGF production and
thymidine incorporation were significantly reduced in the endothelial cells cultured with high concentrations of
glucose . -
pubmed
Biochemical and thrombolytic properties of a low molecular weight form ( comprising
Leu144 through
Leu411 ) of
recombinant single - chain urokinase - type plasminogen activator . -
pubmed
rscu - PA-32k is very similar to intact recombinant single - chain
urokinase - type plasminogen activator in terms of its very low activity ( 120 IU / mg ) on a chromogenic substrate for
urokinase (
pyroglutamylglycylarginine p - nitroanilide ) , its
plasminogen - dependent fibrinolytic activity on
fibrin plates ( specific activity = 170,000 IU / mg ) , its
plasminogen activating potential , and the lack of specific binding to
fibrin . -
pubmed
In contrast , although 0.1 mM
chloroquine , an endosomal - lysosomal inhibitor , also depressed degradation ( by 57 % ) , intracellular
insulin increased fourfold , indicating failure of intracellular processing . -
pubmed
Furthermore , the intracellular degradative processing of
insulin by these cells involves a
chloroquine - sensitive pathway . -
pubmed
With perfusates containing 0.83 - 1.21 mM
Ca2 + ( equivalent to 8 - 11 mg / dl serum
calcium ) , the number of type - I storage granules ( large core ) [ NSG - I ] and that of type - II storage granules ( small core ) [ NSG -
II ] remained unchanged . -
pubmed
On the contrary , with perfusates containing 1.27 mM
Ca2 + ( 11.5 mg / dl ) or more , NSG -
II increased and the ratio of NSG - I to NSG -
II was changed reversely . -
pubmed
We concluded that a threshold value of CC required for the release of storage granules may be present between 0.88 and 0.83 mM
Ca2 + ( 8 and 7.5 mg / dl ) and that a threshold value of CC for accelerating the transformation of type - I granules into type -
II , the degradation of storage granules , may be situated at about 1.27 mM
Ca2 + ( 11.5 mg / dl ) . -
pubmed
The tissue level of endogenous
prostaglandins seems to exert protection since administration of
cyclooxygenase inhibitors ,
indomethacin and
aspirin , markedly increased the incidence of
cecitis . -
pubmed
The following agents were inactive :
loperamide , an antidiarrheic agent ;
carbachol , a cholinergic and diarrheogenic agent ,
atropine , an anticholinergic agent ; and
acetazolamide , a
carbonic anhydrase inhibitor . -
pubmed
The amount of this extra
Phe -
tRNA form is greatest in slowly growing cells ( 0.8 generations / h ) , and becomes negligible in media supporting a rapid growth rate ( 2.14 generations / h ) . -
pubmed
The specific
enzyme activities in microsomes prepared by ultracentrifugation and by
calcium aggregation were not significantly different , although the ultracentrifugation method resulted in a higher yield of hepatic microsomal
protein . -
pubmed
The
enzyme activities in microsomes prepared by ultracentrifugation / or
calcium aggregation and frozen at -20 degrees C up to 22 days were not significantly different from those of freshly prepared microsomes . -
pubmed
dDAVP , a non - pressor antidiuretic analogue to
ADH , induced 1 ) a high urine concentration ( 2,645 + /- 44 ( SEM ) in group E vs 131 + /- 6 mosmol / kg
H2O in group C ) , P less than 0.001 ; 2 ) no significant change in plasma osmolality ( 288 + /- 2 vs 297 + /- mosmol / kg
H2O respectively ) and in plasma concentration of major
electrolytes ,
Na , K ,
Cl ,
Mg , and
Ca ; 3 ) a large decrease in urinary excretion of
calcium and
magnesium and no change in other
electrolyte or total osmolar excretion . -
pubmed
Testosterone levels before and after
HCG administration and
LH and
FSH responses to
LH - RH were measured at the first visit and a few years later . -
pubmed
Incubation for 48 - 72 h in culture medium supplemented with 2 mM -
fructose and 0.1 microM -
insulin induced the synthesis of
L - type pyruvate kinase , as judged by increased enzyme activity and the increased incorporation of
[ 3H ] leucine into immunoprecipitable
L - type pyruvate kinase . -
pubmed
The activated first component of complement ( CI ) possesses an
esterase activity in vitro which will hydrolyse an
ester of
tyrosine to release H+ . -
pubmed
Disulfide mutants of
barnase . -
pubmed
In this series of papers , we examine the effects of introducing
disulfide bonds on the properties , structure and thermodynamics of a small globular protein ,
barnase . -
pubmed
Two of the
disulfide bonds , between residues 43 and 80 ( 43 - 80 ) and between residues 85 and 102 ( 85 - 102 ) , stabilise the
protein , relative to both wild - type and the corresponding ( reduced )
dithiol forms : 85 - 102 is more stable than predicted from the entropic destabilisation of the unfolded state ; 43 - 80 is less stable than predicted . -
pubmed
The third
disulfide bond , between residues 70 and 92 ( 70 - 92 ) destabilises the
protein relative to both wild - type and the corresponding
dithiol form , implying significant disruption of the folded protein on formation of the
disulfide bond . -
pubmed
The
disulfide bond at 43 - 80 induces small structural rearrangements close to the site of the
disulfide bond , associated with some local disorder : the crosslink appears to decrease the stability of the native form of the
protein . -
pubmed
Inhibition of
nitric oxide synthase activity in cerebral cortical synaptosomes by
nitric oxide donors : evidence for feedback autoregulation . -
pubmed
,
NOS activity ) was found to be highly
calcium - dependent and strongly inhibited ( up to 60 percent ) by
NO donors , including
sodium nitroprusside ,
hydroxylamine and
nitroglycerin . -
pubmed
L - Citrulline , the other major end - product from
NOS , had no apparent effect on synaptosomal
NOS activity . -
pubmed
Taken together , these results indicate that neuronal
NOS can be inhibited by
NO released from exogenous donors and , therefore , may be subject to end - product feedback inhibition by
NO that is formed locally within neurons or released from proximal cells . -
pubmed
Investigation of the role of signal transduction in attachment of ocular
melanoma cells to matrix proteins : inhibition of attachment by
calmodulin antagonists including
tamoxifen . -
pubmed
Preferred substrates of attachment were
collagens type I , III and IV and
fibronectin rather than
laminin ,
gelatin ,
arginine -
glycine -
aspartine ,
vitronectin ,
poly - L - lysine or plastic . -
pubmed
Manipulation of intracellular
cyclic AMP or
protein kinase C activity had relatively little effect on cell attachment . -
pubmed
In contrast , attachment was significantly reduced by manipulating either intracellular
calcium or
calmodulin . -
pubmed
The experimental
calmodulin antagonist J8 ( 25 microM ) , a substituted
naphthalene sulphonamide , reduced attachment to 40 % . -
pubmed
Similarly
tamoxifen ( 25 microM ) , which has
calmodulin antagonist activity in vitro , reduced attachment to 55 % . -
pubmed
Both J8 and
tamoxifen inhibited cell attachment to a wide range of matrix proteins , suggesting that this effect on attachment is not dependent on the presence of specific
adhesion receptors . -
pubmed
Reduction of ocular
melanoma tumour cell / matrix interactions through manipulation of intracellular
calcium or
calmodulin may therefore merit further investigation as a possible approach to reducing metastatic spread . -
pubmed
Bilirubin concentrations higher than 376 mumol / L for
apo AI and 444 mumol / L for
apo B , produce a negative interference . -
pubmed
In a second approach , we have determined the capability of three toxic or
metabolic injuries to induce
LNGFR expression without any direct
trauma of the nerve ( experimental diabetogenesis , botulinum and
alpha - bungarotoxin intoxication and
2,5 - hexanedione intoxication ) . -
pubmed
In a third approach , we have investigated the effect of the block of the axoplasmic transport on the
LNGFR expression following different topical applications of
vincristine combined with a nerve crush . -
pubmed
When
glucose is present ,
GABA caused a dose dependent inhibition of the first phase of
arginine - induced
insulin secretion during the range of 10 - 1000 microM , but
GABA did not affect
arginine - induced
insulin secretion in the absence of
glucose . -
pubmed
GABA inhibited not only the first phase but also the second phase of
glucose - induced
insulin secretion . -
pubmed
A
GABAB - receptor agonist ,
baclofen , also inhibited both phases of
insulin secretion induced by 16.7 mM
glucose . -
pubmed
These inhibitory effects of
GABA on
insulin secretion may be mediated through
GABAB - receptor and the inhibition of the rise in cytoplasmic
Ca2 + . -
pubmed
In 10 mM
sodium phosphate , pH 7.6 , containing 0.1 mM
ethylenediaminetetraacetic acid , ions correspondings to the non - calent , four - stranded oligonucleotide , d(CGCG4GCG)4 , were detected by negative ion electrospray ionization ( ESI ) mass spectrometry at a low nozzle - skimmer ( delta
NS ) bias ( -150 V ) , but not at a higher delta
NS bias ( & gt ; -250 V ) . -
pubmed
The postoperative course was uneventful , with complete recovery after a long period of treatment with
penicillin (
IV and PO ) . -
pubmed
Neither intimal nor medical SMC replication rates were decreased by
GM 6001 treatment , supporting our hypothesis that the decrease in lesion size was due to inhibition of
MMP - mediated migration and not inhibition of replication . -
pubmed
Elevation of the markers of fibrinolysis ,
plasmin plasmin inhibitor complex (
PIC ) and
fibrin degradation products ( FDP ) , was slower than that of
TAT , peaking 7 days after treatment in both groups , the plasma
PIC being significantly enhanced 7 days after treatment in the sclerotherapy group . -
pubmed
The structure supports the hypothesis that the highly conserved
tripeptide could play a key role in the interaction of
Hsp40 with the molecular
chaperone ,
Hsp70 . -
pubmed
AML samples were studied with the
MDR1 - specific MAbs MRK16 and 4E3 , and the efflux function was analyzed using
Rh-123 retention in the absence or presence of
verapamil . -
pubmed
The
MDR1 /
beta 2 - M mRNA ratios for
32P - PCR were 0.41 , 0.40 and 0.12 , respectively , and were 0.127 , 0.097 and 0.028 , respectively , for QPCR . -
pubmed
Peroxisomal beta - oxidation and
carnitine acetyltransferase activities were increased 11- and 20 - fold , respectively , after 5 days of treatment with
DHEAS ( 40 microM ) . -
pubmed
Immunoblot and Northern blot analyses of
acyl - CoA oxidase revealed that suppression by
nicardipine of the induction of peroxisomal beta - oxidation activity would be responsible for an increase in the amount of mRNA . -
pubmed
In addition , the manner in which
nicardipine suppressed the induction of peroxisomal beta - oxidation and
carnitine acetyltransferase activity , was similar to that of
clofibric acid . -
pubmed
A direct effect of
CO on the activity of
calcium - activated K ( KCa ) channels in vascular smooth muscle cells ( SMCs ) and the underlying mechanisms were investigated in the present study . -
pubmed
Furthermore , the effect of
CO on KCa channels was not mediated by
cGMP or
guanine nucleotide - binding proteins (
G proteins ,
Gi / Go or Gs ) in excised membrane patches . -
pubmed
An
alanine - scanning study for an
NS5A / 5B substrate ( P6P4 ' ) revealed that
P1 Cys and
P3 Val were critical . -
pubmed
Finally , substitutions at the scissile
P1 Cys residue by
homocysteine (
Hcy ) ,
S - methylcysteine (
Mcy ) ,
Ala ,
S - ethylcysteine (
Ecy ) ,
Thr ,
Met ,
D - Cys ,
Ser , and
penicillamine (
Pen ) produced progressively less efficient substrates , revealing a stringent stereochemical requirement for a
Cys residue at this position . -
pubmed
Both
ir - ACTH and ir - beta E levels increased 5 and 10 min after
ether inhalation , but the increase in the ir - beta E concentration was only half that of the
ir - ACTH . -
pubmed
Compounds with
-( CH2 ) 2- side chains terminating in basic groups such as
piperidine show inhibition of
telomerase at telIC50 levels of 4 - 11 microM. -
pubmed
Implications for
amidoanthracene-9,10 - dione telomerase inhibitors as potential anticancer agents are discussed . -
pubmed
The following deductions can be made : ( 1 ) Cell
injury activates enzymes :
lipoxygenases generate lipid
hydroperoxides (
LOOHs ) ,
proteases liberate
Fe ions -- these two processes are prerequisites to produce radicals . -
pubmed
LOHs derived from
LA ,
hydroxyoctadecadienoic acids (
HODEs ) , surmount other markers of
LPO . -
pubmed
Special problems associated with the use of non - B - cell somatic cells include the processing of
proinsulin to
insulin , and the conferment of sensitivity to
glucose - stimulated
proinsulin biosynthesis and regulated
insulin release . -
pubmed
Regional population - specific influences have been found to affect the
vitamin - D - endocrinologic - system , diminishing the influence of
VDR polymorphism on
BMD and on bone turnover . -
pubmed
The morning following initial specimen collections , both urinary excretion rate of
pyridinoline crosslinks (
Pyr ) and serum levels of
bone alkaline phosphatase (
BAP ) were determined . -
pubmed
The
beta ( 1 )/ beta ( 2 )- chimeric receptors showed the importance of the second and seventh transmembrane domains ( TM2 and TM7 ) of the
beta ( 2 ) AR for the binding of the beta(2)-selective agonists such as
formoterol and
procaterol . -
pubmed
Alanine - substituted mutants of TM7 of the
beta ( 2 ) AR showed that
Tyr(308 , ) located at the top of TM7 , mainly contributed to
beta ( 2 ) selectivity . -
pubmed
In contrast to
beta ( 2 ) AR , TM2 is a major determinant that beta(1)-selective agonists such as
denopamine and
T-0509 bound the
beta ( 1 ) AR with high affinity . -
pubmed
Three
amino acids (
Leu(110 ) ,
Thr(117 ) , and
Val(120 ) ) in TM2 of the
beta ( 1 ) AR were identified as major determinants for beta(1)-selective binding of these agonists . -
pubmed
Three - dimensional models built on the basis of the predicted structure of
rhodopsin showed that
Tyr(308 ) of the
beta ( 2 ) AR covered the binding pocket formed by TM2 and TM7 from the upper side , and
Thr(117 ) of the
beta ( 1 ) AR located in the middle of the binding pocket to provide a
hydrogen bonding for the beta(1)-selective agonists . -
pubmed
Phase
II / III trials testing these new agents in combination with
cisplatin have been carried out in recent years with inconsistent results . -
pubmed
Structural implications of the chemical modification of
Cys(10 ) on
actin . -
pubmed
Cys(10 ) is located in subdomain 1 of
actin , which has an important role in the interaction of
actin with
myosin- and
actin - binding proteins . -
pubmed
The specificity of
Cys(10 ) modification was verified by showing that the 33 - kDa
subtilisin fragment of
actin ( residues 48 - 375 ) , which contains all of the
actin thiols but
Cys(10 ) , is not fluorescent . -
pubmed
Cys(10 ) modification exposed a new site on
actin to
subtilisin cleavage . -
pubmed
The modification slightly increased the rate of
epsilonATP -
ATP exchange and decreased the rates of
G - actin ATPase and polymerization . -
pubmed
The activation of
S1 ATPase by
Cys(10)-modified
F - actin showed small probe - dependent changes in the values of V(max ) and K(M ) . -
pubmed
The sliding speed of
actin filaments in the in vitro motility assay remained unchanged upon modification of
Cys(10 ) . -
pubmed
These results indicate that although the labeling of
Cys(10 ) perturbs the structure of subdomain 1 , the modified
actin remains fully functional . -
pubmed
The binding of
S1 to
actin filaments decreases the accessibility of
Cys(10 ) probes to
acrylamide and
nitromethane quenchers . -
pubmed
Because
Cys(10 ) does not participate directly in either
actin polymerization or
S1 binding , our results indicate that
actin - actin and
actin -
myosin interactions induce dynamic , allosteric changes in
actin structure . -
pubmed
No significant correlations were found between
plasma vasopressin and plasma
sodium or osmolality levels , but there were significant correlations between
plasma vasopressin and urine osmolality , 36 ( r=0.74 , p=0 . -
pubmed
Blockade of STN neuronal activity by
muscimol (
GABA ( A ) receptor agonist ) injection resulted in abolishment of both the early and late excitations evoked in
GP neurons by cortical stimulation . -
pubmed
Injection of
( + /-)- 3 -( 2 - carboxypiperazin-4 - yl )- propyl-1 - phosphonic acid (
CPP )
[ N - methyl - D - aspartate ( NMDA ) receptor antagonist ] , but not
1,2,3 , 4 - tetrahydro-6 - nitro-2,3 - dioxo - benzo [ f ] quinoxaline-7 - sulfonamide disodium [
NBQX ( non -
NMDA receptor antagonist ) ] , into the STN attenuated the early and late excitations in
GP neurons , suggesting that cortico - subthalamic transmission is mediated mainly by
NMDA receptors . -
pubmed
Interference with the pallido - subthalamic transmission by
bicuculline (
GABA ( A ) receptor antagonist ) injection into the STN made the inhibition distinct without affecting the early excitation . -
pubmed
Radioligand binding assays with
( 3 ) H-1 -( 2 -( diphenylmethoxy )- ethyl )- 4 -( 3 - phenylpropyl ) piperazine (
( 3 ) H - GBR12935 ) demonstrated that
iron deficiency did not alter the affinity of the ligand for the
DAT but did significantly decrease the density of the transporter by 30 % in caudate putamen and 20 % in nucleus accumbens . -
pubmed
RESULTS : Loss of
p53 function (
p53 - LOF ) , defined as a failure to induce
p21 and/or
MDM2 in response to
melphalan , was seen in 1/8 drug - sensitive and 6/10 drug - resistant cell lines . -
pubmed
These results suggest that
desaturase genes could have , at least in part , independent control mechanisms and that
Delta-6 desaturase impairment is not specific to any particular step of the
fatty acid metabolic pathways , which may diminish the rationale for the existence of at least two distinct enzymes . -
pubmed
We studied effects of haplotypes comprising 3 loci ( 5 ' to 3 ' : PvuII ,
HindIII , and
Ser 447 -Stop ) in the
lipoprotein lipase (
LPL ) gene on plasma lipid levels and
LPL activity , in 807 Dutch males with
coronary atherosclerosis . -
pubmed
BFnon - HCO3 was 35 + /- 4 mM pH-1 for the
EDL ( n = 14 ) and 86 /- 16 mM pH-1 for the soleus ( n = 14 ) . -
pubmed
In soleus , 10 mM
cinnamate inhibited
lactate influx by 44 % and efflux by 30 % ; in
EDL , it inhibited
lactate influx by 37 % and efflux by 20 % . -
pubmed
Cinnamate decreased [
lactate]i , in soleus by 36 % and in
EDL by 45 % . -
pubmed
In
EDL ,
DIDS lowered the influx by 27 % but had almost no effect on efflux . -
pubmed
DIDS reduced [
lactate]i by 20 % in soleus and by 26 % in
EDL . -
pubmed
However , substitution of
A9 leads to relatively small changes in folding properties and cleavage activity , and the double variant G+1DAP / C25U (
DAP is
2,6 - diaminopurine ) , which could form an isosteric triple - base interaction , exhibits folding and cleavage activities that are both very impaired compared to those of the natural sequence . -
pubmed
The results are interpreted in terms of an interaction between the
ribose ring of
C25 and the
ribose and base of
A10 , in agreement with the proposal of Ryder and Strobel [ Ryder , S. P. , and Strobel , S. A. -
pubmed
An exception to this is provided by
G8 , for which substitution with
uridine leads to severe impairment of cleavage but folding characteristics that are virtually unaltered from those of the natural species . -
pubmed
This is consistent with a direct role for the
nucleobase of
G8 in the chemistry of cleavage . -
pubmed
Bradykinin (
BK ) and
vascular endothelial growth factor ( VEGF )- 165 stimulate vasodilatation , microvascular permeability , and angiogenesis via the activation of the
B2 - type and KDR / Flk-1 receptors . -
pubmed
The two mediators induced a similar cytoskeletal reorganization and both the translocation and activation of
eNOS , leading to
NO release within the first minutes of cell exposure . -
pubmed
At the same time ,
BK produced the
tyrosine phosphorylation and internalization of
KDR /
Flk-1 as did
VEGF itself . -
pubmed
The selective inhibitor of
VEGF receptor tyrosine kinase activity totally prevented the effects of
VEGF but only partially inhibited
NO release induced by
BK without affecting the concomitant cytoskeletal reorganization . -
pubmed
Minor effects of
ACEA on
Abeta - fiber - evoked responses were observed . -
pubmed
Spinal pre - administration of the selective
CB1 receptor antagonist
SR141716A ( 0.01 microg/50 microl ) significantly reduced effects of
ACEA ( 500 ng/50 microl ) on postdischarge responses of dorsal horn neurons . -
pubmed
On the basis of polarity , we presumed that the unidentified lipid was a product of the
lipoxygenase (
LOX ) pathway and that inclusion of the characteristic
LOX inhibitors
nordihydroguaiaretic acid and
eicosatetraynoic acid reduced its formation in vitro and in vivo . -
pubmed
Cell fractionation studies showed that the
NAE amidohydrolase , responsible for
FFA production , was associated mostly with microsomes , whereas
LOX , responsible for
NAE 18:2 - oxylipin production , was distributed in cytosol - enriched fractions and microsomes . -
pubmed
The highest activity toward
NAE by
amidohydrolase was observed 4 to 8 h after imbibition and by
LOX 8 h after imbibition . -
pubmed
Investigation of
pulmonary function among employees exposed to low levels of monomeric
isocyanates and solvents at an automobile finishings plant . -
pubmed
There have been reports in the literature of decrements in
pulmonary function associated with long - term , low - level monomeric
isocyanate exposure combined with solvent exposure . -
pubmed
The results of the analysis demonstrated no statistically significant relationship between combined
isocyanate and solvent exposure and decline in
pulmonary function . -
pubmed
Four of 13 patients with definite
recurrence and 14 of 18 patients with no
recurrence were administered
azathioprine (
AZA ) as part of their
post - LT therapy ( P = .01 ) . -
pubmed
No significant differences were seen with donor - recipient group A , group B , group O blood type , sex , or
HLA mismatches ; native liver histological characteristics ; or
tacrolimus - based therapy . -
pubmed
Catalase ,
glycolate oxidase and
hydroxypyruvate reductase as well as enzymes of the
fatty acid beta - oxidation pathway were demonstrated to be constituents of the microbodies in Chara indicating that they are similar to those in green leaves . -
pubmed
BACKGROUND AND OBJECTIVES : Serum soluble
intercellular adhesion molecule-1 (
sICAM-1 ) and total
sialic acid (
TSA ) are related to the metastatic potential of
cancer cells . -
pubmed
The purpose of the present investigation was to determine
sICAM-1 and
TSA levels in colorectal
carcinoma and correlate their levels with the
cancer stage . -
pubmed
The concentrations of
sICAM-1 and
TSA were measured by enzyme - linked immunoassay and the
thiobarbituric acid method , respectively , and compared with those from a healthy control group ( n = 42 ) . -
pubmed
CONCLUSIONS : The results of this investigation indicate that
sICAM-1 and
TSA are the best of the tested markers . -
pubmed
Qunatitative determination of the minor
hemoglobin component
Hb - A2 by
DEAE - cellulose chromatography . -
pubmed
STUDIES OF SERUM
CHOLESTEROL IN 4,244 MEN AND WOMEN : AN EPIDEMIOLOGICAL AND PATHOGENETIC INTERPRETATION . -
pubmed
2NMP.3H ( 2 ) O ( Ln = Ce ( IV ) ( 1 ) ,
Pr ( IV ) ( 2 ) , x = 0 ;
Ln =
Nd ( III ) ( 3 ) , x = 1 ;
NMP = N - methyl-2 - pyrrolidone ) have been prepared in aqueous solution and characterized by elemental analyses , IR , UV - vis , and
TG analyses . -
pubmed
Adiponectin may also be responsible for the effectiveness of
thiazolidinediones , given that these drugs promote
adiponectin secretion . -
pubmed
Mobility of
Zn ,
Cd and
Pb in soils as affected by poultry litter extract --
II . -
pubmed
After leaching , the soil samples in the columns were sequentially extracted for water soluble (
WS ) , exchangeable ( EXC ) , organic matter (
OM ) ,
Mn oxide ( MNO ) , amorphous
Fe oxide ( AFEO ) , crystalline
Fe oxide ( CFEO ) and residual ( RES ) fractions . -
pubmed
The
OM fraction showed high retention for
Zn from the PLE . -
pubmed
The
EDTA redistributed
Zn ,
Cd and
Pb from the EXC ,
OM and
MNO fractions to the WS fraction . -
pubmed
Samples were analyzed for total
cholesterol ,
triacylglycerides ,
high - density lipoprotein -
cholesterol , and
low - density lipoprotein -
cholesterol . -
pubmed
The production of
estrogen from
androgen via the
estrogen biosynthesis pathway is catalyzed by
aromatase P450 (
CYP19 ) . -
pubmed
There was no association between
CYP19 gene polymorphism and
breast cancer risk in the study group as a whole , but homozygous and heterozygous carriers of the variant
Arg allele showed a significantly increased risk of
breast cancer among premenopausal women with a late age at first full - term pregnancy ( OR 7.31 , 95 % CI 1.88 - 28.5 ) or a high
body mass index ( OR 2.77 , 95 % CI 1.12 - 6.87 ) . -
pubmed
Likewise , both the
ether extract and
alpha - amyrin given topically dose - dependently prevented the increase of the proinflammatory
cytokine interleukin-1beta levels in response to topical application of
TPA . -
pubmed
The exposure of VSMC to
ursolic acid results in a chemotaxis inhibition , in a reduction of the expression of
proliferating cell nuclear antigen (
PCNA ) and in a disorganization of
beta - tubulin and
vimentin cytoskeletal proteins . -
pubmed
Pentachlorophenol degradation occurred in both planted and unplanted
pots , but a higher rate of degradation was observed in the planted
pots . -
pubmed
In pure
DMF , all complexes except the
Zn ( II ) compounds exhibit metal - centered M(III /
II ) (
Mn ,
Fe ,
Co ,
Ni ) or
M ( II / I ) ( Cu ) redox processes . -
pubmed
Fluorescence anisotropy indicated that
NaCl decreased the fluidity of the external
face of the plasmalemma but increased the fluidity of cell membranes in general . -
pubmed
Initial isolation of whole
APP was performed by immunoprecipitation , using AB10 , a monoclonal antibody raised to
amino acids 1 - 17 of the beta - amyloid peptide sequence , which is present in all three
alpha secretase - cleaved isoforms of interest . -
pubmed
Here we will discuss data supporting the view that
steroids bring about meiotic maturation through functionally redundant pathways involving synthesis of
Mos or of
cyclin proteins , reinforcing the robustness of the system . -
pubmed
Ion trap
MS /
MS of intact
testosterone and
epitestosterone conjugates -- adducts , fragile ions and the advantages of derivatisation . -
pubmed
Both compounds form multiple adducts by ESI -
MS , namely protonation ,
ammonium and
sodium , however , the mass spectrum of EG displays a more intense
ammonium adduct peak than
TG . -
pubmed
Ammonium adduction was localised to the
glucuronide ring
oxygens and while EG has eight possible adduction sites , only seven were located for
TG explaining the increased
ammonium adduct abundance with EG . -
pubmed
AG014699 and
temozolomide pharmacokinetics ,
PARP activity , DNA strand single - strand breaks , response , and toxicity were evaluated . -
pubmed
METHODS :
PBC was classified as early ( pretreatment
bilirubin and
albumin levels normal ) , moderately advanced ( one level abnormal ) , or advanced ( both levels abnormal ) . -
pubmed
Biochemical response was defined as proposed by Pares ( decrease in
alkaline phosphatase [
ALP ] level>40 % of baseline level or normal level ) , Corpechot (
ALP level<3 - fold the upper limit of normal [ ULN ] ,
aspartate aminotransferase level&
lt;2 - fold the ULN ,
bilirubin level<1 - fold the ULN ) , and our group ( Rotterdam ; normalization of abnormal
bilirubin and/or
albumin levels ) . -
pubmed
Prognostic information , based on
bilirubin and
albumin levels , is superior to that provided by
ALP levels . -
pubmed
Surprisingly , loss of the
Git3 glucose receptor , a G protein - coupled receptor , also increased life span in conditions where
glucose consumption was not affected . -
pubmed
A similar pro - aging effect of
glucose was documented in mutants of
hexokinase , which can not metabolize
glucose and , therefore , are exposed to constitutive
glucose signaling . -
pubmed
Likewise , the anti - aging effect of both calorie restriction and the
Deltagit3 mutation was accompanied by increased respiration and lower reactive
oxygen species production . -
pubmed
By immunohistochemistry , we also show that both
transforming growth factor beta and the mammalian target of
rapamycin are induced by constitutive activation of
beta - catenin . -
pubmed
In subjects in group A ,
glucose excursions were induced on one study day and those in group B underwent a
glucose clamp ( eu- , hypo- or
hyperglycaemic ) on one study day . -
pubmed
CRT data were calibrated once with a retrospective calibration model based on a run - in time of 24 h and three blood
glucose measurements per day . -
pubmed
RESULTS : All analysable experiments , covering a broad range of blood
glucose values , yielded highly accurate data for the complete experimental time with a mean relative absolute difference of 12.8 + /- 6.0 % and a predictive residual error sum of squares of 15.6 + /-
6.3 ( mean + /- SD ) . -
pubmed
The
CBL - CIPK
Ca ( 2 +)-decoding signaling network : function and perspectives . -
pubmed
The family of
calcineurin B - like (
CBL ) proteins represents a unique group of
calcium sensors and contributes to the decoding of
calcium transients by interacting with and regulating the family of
CBL - interacting protein kinases (
CIPKs ) . -
pubmed
Impact of phospholipid bilayer saturation on
amyloid - beta protein aggregation intermediate growth : a
quartz crystal microbalance analysis . -
pubmed
In the current study , a
quartz crystal microbalance ( QCM ) was employed to examine the growth of
Abeta(1 - 40 ) aggregation intermediates on supported phospholipid bilayers ( SPBs ) assembled at the crystal surface . -
pubmed
Growth - capable
Abeta ( 1 - 40 ) aggregation intermediates more readily bind SPBs composed of phospholipids with a greater degree of
carbon saturation . -
pubmed
CUS ( 5 - 6 - week exposure to stressors ) , but not sub - CUS ( 1 week exposure to stressors ) , induces
depression - like behaviors and impairs these forms of eCB /
CB1 receptor - mediated plasticity examined in the NAc core . -
pubmed
However , exposure to
CUS , but not to sub - CUS , attenuated the
depression of fEPSPs induced by the
CB1 receptor agonist
WIN 55 212 - 2 . -
pubmed
Thus , impaired
CB1 receptor function could account for
CUS - induced deficiency in eCB signaling in the NAc . -
pubmed
We investigated the effects of a high
TF diet on
TGF - beta responsiveness in aortic endothelium and integration of
cholesterol in tissues . -
pubmed
Despite drastic effects on phenotype and survival ,
nodularin did not disturb the photosynthetic performance of plants or the structure of the photosynthetic machinery in the
chloroplast thylakoid membrane . -
pubmed
Moreover , the high level of
cytochrome oxidase (
COX II ) , a typical marker for mitochondrial respiratory protein complexes , suggests that the respiratory capacity is increased in the leaves of
nodularin - exposed plants . -
pubmed
Sphingosine 1 - phosphate (
S1P ) initiates T and B cell exit from lymphoid tissues by activating the
S1P ( 1 ) receptor on lymphocytes . -
pubmed
Plasma - borne
S1P was obviously not sufficiently inactivated by
Sphingomab to account for the observed
lymphopenia . -
pubmed
Therefore , we addressed the local
S1P - blocking activity of
Sphingomab in spleen and peripheral lymph nodes ( pLNs ) as a potential cause of PBL depletion . -
pubmed
In addition , exposure to
rGal-1 markedly accelerated the development of apical bile canaliculi as shown by
TRITC -
phalloidin labeling and immunostaining for
multidrug resistance associated - protein 2 (
MRP2 ) . -
pubmed
Notably ,
rGal-1 did not interfere with
multidrug resistance protein 1 /
P - glycoprotein or
MRP2 apical localization , neither with transfer nor secretion of
5 - chloromethylfluorescein diacetate through
MRP2 . -
pubmed
Stimulation of cell adhesion and polarization by rGal-1 was abrogated in the presence of
thiodigalactoside , a
galectin - specific
sugar , suggesting the involvement of protein -
carbohydrate interactions in these effects . -
pubmed
Additionally ,
Gal-1 effects were abrogated in the presence of
wortmmanin ,
PD98059 or
H89 , suggesting involvement of
phosphoinositide 3 - kinase (
PI3 K ) ,
mitogen - activated protein kinase and
cyclic adenosine monophosphate - dependent protein kinase signaling pathways in these functions . -
pubmed
The deduced
amino acid sequence of
AbnS1 had 70 % identity with that of
Abnc . -
pubmed
In contrast , expression of the
abnc gene encoding
Abnc was strongly induced by
arabinose ,
arabinitol , and arabinan . -
pubmed
Using debranched arabinan as substrate , recombinant
AbnS1 (
rAbnS1 ) accumulated
arabinobiose and
arabinotriose as the major products . -
pubmed
Recombinant
Abnc (
rAbnc ) released mainly
arabinotriose and lesser amounts of
arabinose and
arabinobiose than did
rAbnS1 . -
pubmed
Branched arabinan was completely degraded to
arabinose by the action of
rAbnS1 or
rAbnc in combination with
alpha - L : - arabinofuranosidase . -
pubmed
Immune function test ( Immuknow ) is a measure of cell - mediated immunity based on peripheral
CD4 + T cell
adenosine triphosphate activity ( desired range , 225 - 525 ng / mL ) . -
pubmed
A randomized , double - blind , phase III study of
Docetaxel and Ramucirumab versus
Docetaxel and placebo in the treatment of stage
IV non - small - cell lung cancer after
disease progression after 1 previous
platinum - based therapy ( REVEL ): treatment rationale and study design . -
pubmed
This article describes the treatment rationale and study - related procedures for the A Randomized , Double - Blind , Phase 3 Study of
Docetaxel and Ramucirumab Versus
Docetaxel and Placebo in the Treatment of Stage
IV Non - Small - Cell Lung Cancer Following Disease Progression after One Prior
Platinum - Based Therapy ( REVEL ) study ( I4T - MC - JVBA ; ClinicalTrials.govNCT01168973 ) . -
pubmed
The successful formation of DA - stabilized
copper particles was demonstrated by ultraviolet - visible spectroscopy ( UV - Vis ) , transmission electron microscopy (
TEM ) , Zeta potential measurement , and Fourier transform infrared spectroscopy ( FT - IR ) . -
pubmed
Quercetin enhances the effects of
5 - fluorouracil - mediated growth inhibition and apoptosis of
esophageal cancer cells by inhibiting
NF - kappaB. -
pubmed
Annexin V -
FITC /
propidium iodide ( PI)-stained fluorescence - activated cell sorter ( FACS ) analysis was used to detect the apoptotic fraction of treated cells , and western blot analysis was used to examine the protein levels . -
pubmed
HEK -
CaSR stimulation with
PA or
Ca ( 2 + ) resulted in greater
pERK activation than that of stimulated
HEK - WT cells . -
pubmed
In addition , the
DgWRKY3 transgenic plants reduced accumulation of
malondialdehyde (
MDA ) and
hydrogen peroxide (
H2O2 ) compared with WT plants , accompanied by higher activities of antioxidant enzymes including
superoxide dismutase (
SOD ) ,
peroxidase (
POD ) ,
catalase (
CAT ) , and
ascorbate peroxidase (
APX ) and the greater accumulation of antioxidants including
ascorbate (
AsA ) and
glutathione (
GSH ) under salt stress . -
pubmed
AIM : Increased body
iron is associated with
insulin resistance . -
pubmed
Hepcidin is the key hormone that negatively regulates
iron homeostasis . -
pubmed
Reduced
hepcidin concentrations may cause increased body
iron stores in
insulin - resistant states . -
pubmed
Finally induction of apoptosis by
3 - MA in
CuO NP treated cells was observed by
TEM . -
pubmed
RESULTS : This study indicates that
CuO NPs are a potent inducer of autophagy which may be a cellular defense against the
CuO NP mediated toxicity and inhibition of autophagy switches the cellular response into apoptosis . -
pubmed
The high concentrations of TOC ,
TN and
BSi indicate the high nutrient level and diatom productivity in Zhelin Bay . -
pubmed
Laboratory tests revealed the presence of a subgenotype
A2 carrying at least seven different mutations within the
S protein and high
lopinavir plasma concentrations . -
pubmed
The effect of thermal treatment of radiation - induced
EPR signals of different polymorphic forms of
trehalose . -
pubmed
Electron paramagnetic resonance (
EPR ) signals induced by gamma - radiation in different polymorphic forms of
trehalose were studied with dosimetry applications in view . -
pubmed
Also , thermal stability and decay kinetics of the
EPR signals of the different forms of
trehalose were studied in isothermal annealing experiments . -
pubmed
The kinetic parameters , which had been derived by fitting the Arrhenius function to the measured decay rate constants , indicated that the fading of the
EPR signals varied from one polymorphic form of
trehalose to another . -
pubmed
Tolvaptan plus
pasireotide shows enhanced efficacy in a
PKD1 model . -
pubmed
Compounds such as
tolvaptan and
pasireotide , which indirectly reduce
adenylyl cyclase 6 (
AC6 ) activity , have hence proven effective in slowing cyst progression . -
pubmed
Initially , the
Pkd1(RC / RC ) model was inbred into the C57BL/6 background , minimizing disease variability , and the pathogenic effect of elevating
cAMP was confirmed by treatment with the
AC6 stimulant
desmopressin . -
pubmed
The river - reservoir transition zone emitted
CH4 at rates an order of magnitude higher than the
rest of the reservoir , and total
carbon emissions ( i.e. -
pubmed
FT - IR , NBO , HOMO - LUMO ,
MEP analysis and molecular docking study of
1 -[ 3 -( 4 - Fluorophenyl )- 5 - phenyl-4,5 - dihydro-1H - pyrazol-1 - yl ] ethanone . -
pubmed
From the
MEP it is evident that the negative charge covers the
carbonyl group and the positive region is over the remaining groups . -
pubmed
From the molecular docking studies , it is evident that the
fluorine atom attached to
benzene ring and
ethanone attached to the
pyrazoline ring are crucial for binding and the compound might exhibit inhibitory activity against
TPII and may act as anti - neoplastic agent . -
pubmed
Electrochemical signals are generated by differential
pulse voltammetry interrogation of
methylene blue (
MB ) that quantitatively binds to surface - confined hairpin probe via electrostatic interactions . -
pubmed
The founding member of
Ca ( 2 + ) binding proteins is
calmodulin (
CaM ) , a small , acidic , modular protein endowed with gymnastic - like flexibility and E - F hand motifs that chelate
Ca ( 2 + ) ions . -
pubmed
In this review , I will trace the history that led to the realization that
CaM serves as the
Ca ( 2 +)-gating cue for SK channels , the experiments that revealed that
CaM is an intrinsic subunit of SK channels , and itself a target of regulation . -
pubmed
We have recently reported on the efficacy of an
N - methyl - d - aspartate ( NMDA ) receptor partial antagonist ,
S - Methyl - N , N - diethylthiolcarbamate sulfoxide (
DETC - MeSO ) , in improving outcome following
stroke , including reduced
infarct size and
calcium influx , suppressing the endoplasmic reticulum (
ER ) stress - induced apoptosis as well as improving behavioral outcome . -
pubmed
DETC - MeSO was shown to suppress the
protein kinase R - like endoplasmic reticulum kinase (
PERK ) pathway , one of the major
ER stress pathways . -
pubmed
Several studies including ours have provided evidence that
taurine also has neuroprotective effects through reducing apoptosis and inhibiting
activating transcription factor 6 (
ATF6 ) and
inositol requiring enzyme 1 (
IRE-1 ) pathways . -
pubmed
We hypothesized that a combined treatment with
DETC -
MeSO and
taurine would ameliorate
ischemia - induced
brain injury by inhibiting all three
ER stress pathways . -
pubmed
Our study showed that combined
DETC -
MeSO and
taurine , but not
DETC - MeSO alone at the dose used , greatly reduced the
infarct size , improved performance on the neuro - score test and attenuated proteolysis of
alphaII - spectrin . -
pubmed
NeuN expression levels indicated that more neurons were protected in the presence of
DETC - MeSO and
taurine . -
pubmed
Therefore , we conclude that combined treatment with both
DETC -
MeSO and
taurine synergistically inhibits all three
ER stress pathways and apoptosis and therefore can be a novel and effective treatment after
ischemic stroke . -
pubmed
The results indicated that the addition of
ALA to the extender resulted in a higher percentage of motile spermatozoa
post - thaw ( P & lt ; 0.05 ) . -
pubmed
The activities of
superoxide dismutase ,
lactate dehydrogenase ,
glutamic - oxaloacetic transaminase and
catalase improved after adding
ALA to the extender ( P & lt ; 0.05 ) . -
pubmed
At the highest tested concentration level ( 5000 mug L(-1 ) ) , following effects were noted : ( 1 ) inhibition on algae growth at the end of the experiment as indicated by the
chlorophyll a and total biovolume ; ( 2 ) inhibition of photosynthetic efficiency on day 24 as suggested by the maximal
Photosystem II quantum yield ( Fv / Fm ) ; ( 3 ) decrease of the algal diversity on day 24 and 32 as reflected by the Pielou and Shannon - Weiner indices . -
pubmed
GIP response to
MMT decreased to a similar extent after the 2 interventions ( p=0.977 ) . -
pubmed
AIM : To investigate the relationship between serum
uric acid levels and
cardiovascular disease in Asians . -
pubmed
Sugar phosphates were selectively captured onto the surface of the
SiO2@PD -
Ti ( 4 + ) microspheres by chelating with
phosphate groups , and then reacted with
3 - amino-9 - ethylcarbazole via reductive amination based on solid - phase derivatization , which could not only increase the retention and resolution of
sugar phosphates on reversed - phase liquid chromatography ( RPLC ) , but also improve the mass spectrometry (
MS ) sensitivity of
sugar phosphates . -
pubmed
Finally , the
SiO2@PD -
Ti ( 4 + ) microspheres combined with RPLC -
MS were successfully applied to the determination of
sugar phosphates from
hepatocarcinoma cell lines and could even detect the trace
sugar phosphates in thousands of cells . -
pubmed
Colitis was induced by dextran sulfate
sodium (
DSS , 2 % ) ;
alpha2 - AR ligands were injected i.p . -
pubmed
The nonselective
alpha2 - AR agonist
clonidine induced a modest aggravation of DSS - induced
colitis . -
pubmed
In contrast , selective inhibition of
alpha2A - ARs by
BRL 44408 significantly delayed the development of
colitis ; reduced the colonic levels of
MPO and
chemokine ( C - C motif ) ligand 3 ,
chemokine ( C - X - C motif ) ligand 2 (
CXCL2 ) ,
CXCL13 , and
granulocyte - colony stimulating factor ; and elevated that of
tissue inhibitor of metalloproteinases-1 . -
pubmed
In study 2 ( n = 106 ) , mean ( SD ) time - weighted sum of
pain relief and
pain intensity difference scores were 18.2 ( 20.0 ) versus 41.5 ( 21.0 ) at 0 - 12 hours and 10.3 ( 12.0 ) versus 18.4 ( 12.1 ) at 8 - 12 hours for placebo versus
ibuprofen IR /
ER , respectively ( P & lt ; .001 for both ) ; efficacy was sustained over each of the four 12 - hour dosing intervals with
ibuprofen . -
pubmed
Ibuprofen 600 mg IR /
ER provided safe and effective analgesia after single and multiple doses . -
pubmed
Phenylpyrrole - based
HDAC inhibitors : synthesis , molecular modeling and biological studies . -
pubmed
Currently , three
hydroxamate - containing
HDAC pan - inhibitors have been approved as antitumor agents . -
pubmed
RESULTS : We herein present the development of a series of novel
phenylpyrrole - based derivatives stemmed from combined computational and medicinal chemistry efforts to rationally modulate
HDAC1 / 6 isoform selectivity . -
pubmed
We also characterized
ECP neutralizing activity using progressively truncated LPS mutants , and conclude that the polysaccharide moiety and
lipid A portions are required for LPS - mediated neutralization . -
pubmed
cAMP /
PKA pathway appears to mediate short - term inhibitory effect of
TLR2 on
SERT ; however ,
p38 MAPK pathway has been shown to be involved in both short- and long - term
TLR2 effect . -
pubmed
Recognition of
polyproline and a number of noncanonical sequences by
SH3 domains has been extensively studied by crystallography , nuclear magnetic resonance and other methods . -
pubmed
ART administration significantly decreased the average number of
ACF / microscopic field . -
pubmed
Similarly ,
LPO level was decreased and antioxidant activities were enhanced after
ART treatment . -
pubmed
As a result , gate - tunable photoluminescence (
PL ) effect , which is rarely achievable for
MoS2 samples prepared by common CVD or mechanical exfoliation , is demonstrated . -
pubmed
This study provides a simple versatile approach to fabricating monolayered crystals of
MoS2 and other high - quality
transition metal dichalcogenides and could lead to new optoelectronic devices based on gate - tunable
PL effect . -
pubmed
STEM performs the best for gold nanoparticles for all layer thicknesses , while
carbon is best imaged with phase - contrast
TEM for thin layers but
bright field STEM is preferred for thicker layers . -
pubmed
We focused on
glucosylceramide synthase (
GCS ) to demonstrate that the
GCS inhibitor
DL - threo - PDMP resensitizes
cisplatin - resistant germline - derived orthoxenografts to
cisplatin . -
pubmed
Even newly released anti -
arrhythmic drugs , like
ivabradine with
HCN channel as a primary target , block the
hERG potassium current in overlapping concentration interval . -
pubmed
Promiscuous drug block to
hERG channel may potentially lead to perturbation of the action potential duration ( APD ) and
TdP , especially when with combined with polypharmacy and/or
electrolyte disturbances . -
pubmed
Based on this tear instability concept , the
P2Y2 antagonist
Diquas and the mucin secretagogue Mucosta were developed and launched in Japan over the last 5 years , and they have been widely prescribed to patients with
dry eye . -
pubmed
Baseline
18F - FDG PET /
CT as predictor of the pathological response to neoadjuvant therapy in
esophageal cancer : A retrospective study . -
pubmed
Exponential - phase
AOM contained a greater high - MW fraction and more
carbohydrates , and the exponential cells were smaller cells and had a higher EPS content ; these characteristics resulted in a reduced average flux . -
pubmed
The selected physiological parameters were plasma concentrations of thyroid hormones (
thyroxine , T4 and
triiodothyronine , T3 ) , plasma proteins (
prealbumin ,
albumin ,
alpha1 - , alpha2 - , beta - and gamma - globulins ) and selected blood clinical chemical parameters ( BCCPs ) associated with liver and kidney functioning . -
pubmed
Our statistical models indicated that
perfluoroalkyl substances ( PFASs ) and legacy OHCs , such as
polychlorinated biphenyls , organochlorinated pesticides and
polybrominated diphenyl ethers , influenced only a minor fraction of the variation of plasma thyroid hormones ,
prealbumin and CORTf ( 5 - 15 % ) , and partly explained the selected BCCPs ( & lt;26 % ) . -
pubmed
Studies must have reported baseline ( pre- ) and
post - intervention ( or change of )
BMI -
SDS and either fasting
glucose , homeostatic model of
insulin resistance (
HOMA - IR ) ,
alanine aminotransferase (
ALT ) ,
C - reactive protein (
CRP ) , or
interleukin-6 (
IL-6 ) . -
pubmed
From the meta - regression analyses , across all study subsets , greater mean falls in all four parameters , ( HOMA - IR ,
Glucose ,
ALT and
CRP ) were observed with greater mean loss of
BMI -
SDS , but the trends were only statistically significant for HOMA - IR and
CRP ( P=0.003 ; P=0.021 ) . -
pubmed
Active
oxygen species (
AOS ) play key roles in many important catalytic reactions relevant to clean energy and environment . -
pubmed
BACKGROUND : Dietary Guidelines for Americans 2015 - 20 recommend choosing water in place of
sugar - sweetened beverages (
SSB ) . -
pubmed
OBJECTIVES : The
carbohydrate -
insulin model ( CIM ) predicts that increases in fasting and
post - prandial
insulin in response to dietary
carbohydrates stimulate energy intake and lower energy expenditures , leading to positive energy balance and
weight gain . -
pubmed
Their fasting and
post - prandial
glucose and
insulin levels were also measured . -
pubmed
RESULTS : Only the
post - prandial
insulin and fasting
glucose levels followed the CIM 's predictions . -
pubmed
However , because higher fasting
insulin was unrelated to
carbohydrate intake , the overall pattern did not support the model . -
pubmed
Activation of
GPR120 by
TUG891 ameliorated
cisplatin - induced
acute kidney injury via repressing
ER stress and apoptosis . -
pubmed
However , whether
GPR120 agonism exerted potential for
cisplatin - induced
acute kidney injury and the involved mechanisms remained unknown . -
pubmed
In our study , pharmacological activation of
GPR120 by
TUG891 treatment remarkably reduced the elevated serum
creatinine level and attenuated
tubular injury . -
pubmed
Cisplatin triggered
ATF6 ,
PERK and
IRE1 pathways of unfolded protein response ( UPR ) of
ER stress in the injured kidney tissue , as well as the downstream molecules
eIF2alpha ,
ATF4 and
XBP1 . -
pubmed
Protein of
ER stress - mediated apoptosis ,
CHOP , was overexpressed in the
cisplatin group . -
pubmed
Oral application of
TUG891 displayed effective inhibition of
ER stress and apoptosis . -
pubmed
Collectively , activation of
GPR120 by
TUG891 exhibited renal protection against
cisplatin - induced
AKI via suppressing
ER - associated apoptosis in tubular epithelial cells . -
pubmed
The activity of
phenoloxidase decreased significantly when dietary
copper level was 86 - 162 mg / kg , and the expression of
hemocyanin was up - regulated in crab fed the diets with 28 - 162 mg / kg
copper . -
pubmed
The main chain of AER -
A3 was unambiguously determined to be smooth region and hairy region with a chain length ratio of 1:1 characterized by occurrence of ( 1 2)-alpha - Rhap ,
( 1 2,3 )- alpha - Rhap ,
( 1 2,4 )- alpha - Rhap ,
( 1 2,3,4 )- alpha - Rhap , and
( 1 4 )- alpha - GalpA , whereas the branched chain included
T - alpha - Araf ,
( 1 5 )- alpha - Araf ,
( 1 3,5 )- alpha - Araf , T - beta - Galp ,
( 1 3 )- beta - Galp ,
( 1 3,4,6 )- beta - Galp ,
( 1 4 )- Glcp ,
( 1 3 )- Glcp , and
( 1 3 )- Manp . -
pubmed
AER -
A3 had good scavenging activities against
DPPH ,
hydroxyl , and
superoxide radicals . -
pubmed
Women with
hypertension are more often prescribed
angiotensin receptor blockers ( ARB ) ,
thiazide and
thiazide - like diuretics . -
pubmed
Insufficient prescription of medication which could improve the prognosis of the disease (
ACE inhibitors /ARB ,
BB ,
mineralocorticoid receptor antagonist ) have been identified in patients with
hypertension and
CAD ,
MI ,
CHF . -
pubmed
Since
Prxs are known to alter ROS levels by using them as electron acceptor or producing them in their catalytic activity , the impact of this overexpression in redox homeostasis was studied by analyzing the metabolites and enzymes of the
ascorbate -
glutathione cycle . -
pubmed
In relation to the modification induced by
ZePrx overexpression in lignin composition and cellular metabolism , the
carbohydrate composition of the cell wall as well as overall gene expression through RNA - Seq were analyzed . -
pubmed
The obtained results indicate that the overexpression of
ZePrx caused an increase in
syringyl lignin in cell wall stems , suggesting that
ZePrx is relevant for the oxidation of
sinapyl alcohol during lignin biosynthesis , coherently with its
S - peroxidase nature . -
pubmed
The perturbation of cellular redox homeostasis occurring as a consequence of
ZePrx overexpression was kept under control by an increase in
APX activity and a reduction in
ascorbate redox state . -
pubmed
NSCs from embryonic day 14 (
E14 ) were propagated at the presence of
basic fibroblast growth factor and
platelet derived growth factor alpha , and then differentiated in the medium containing
triiodothyronine . -
pubmed
Four extracellular matrix ( ECM ) ,
poly - D - lysine ( PDL ) ,
PDL - laminin ,
fibronectin , and matrigel , were examined for NSC differentiation . -
pubmed
Belantamab mafodotin (
belamaf ;
GSK2857916 ) is a first - in - class antibody - drug conjugate (
ADC ; or immunoconjugate ) that delivers a cytotoxic payload ,
monomethyl auristatin F (
MMAF ) , to
myeloma cells . -
pubmed
The
metabotropic gamma - aminobutyric acid B receptor (
GABABR ) is a G protein - coupled receptor that mediates neuronal inhibition by the neurotransmitter
GABA . -
pubmed
Of the identified features , eight metabolites were significantly perturbed in the
IgG4 -
RD group , including
glyceric acid 1,3 - biphosphate (
1,3 - BPG ) ,
uridine triphosphate (
UTP ) ,
uridine diphosphate glucose (
UDP -
Glc ) or
uridine diphosphate galactose (
UDP - Gal ) ,
lysophospholipids ,
linoleic acid derivatives and
ceramides . -
pubmed
The interplay between a
GC - rich
oligonucleotide and
copper ions on
prion protein conformational and phase transitions . -
pubmed
The
prion protein (
PrP ) misfolding to its infectious form is critical to the development of
prion diseases , whereby various ligands are suggested to participate , such as
copper and nucleic acids ( NA ) . -
pubmed
Since
Cu ( II ) is a physiological ligand of
PrP , we argue whether it modulates phase separation altogether with nucleic acids . -
pubmed
Using recombinant
PrP , we investigate the effects of
Cu ( II ) ( at 6 M equivalents ) and a previously described
PrP - binding CG - rich DNA ( equimolarly to protein ) on
PrP conformation , oligomerization , and phase transitions using a range of biophysical techniques . -
pubmed
Our results show that DNA can be an adjuvant , leading to the structural conversion of
PrP , even in the presence of an endogenous ligand ,
copper . -
pubmed
These results provide new insights into the role of
Cu ( II ) and NA on the phase separation , structural conversion , and aggregation of
PrP , which are critical events leading to neurodegeneration . -
pubmed
Serotonylation of
glutamine 5 on
histone H3 ( H3Q5ser ) was recently identified as a permissive posttranslational modification that coexists with adjacent
lysine 4 trimethylation ( H3K4me3 ) . -
pubmed
Removal and culture of
post - partum ligaments , which undergo a 75 % reduction in length and
hydroxyproline content by the fifth
post - partum day , allowed the demonstration of high
collagenase levels released into the media . -
pubmed
The rapid
post - partum ligament resorption could be partially inhibited if
estrogen was injected immediately following parturition , whereas
progesterone or
relaxin significantly impaired ligament resorption . -
pubmed
In a corresponding fashion , when the
collagenase levels of these ligaments were measured
progesterone treatment was shown to inhibit
collagenase activity markedly while
estrogen was less effective . -
pubmed
Other investigations for small - bowel disease may include the breath test with
carbon-14 - labelled
glycocholic acid , the
lactose tolerance test , duodenal aspiration for
giardiasis , analysis of
serum immunoglobulins and , on occasion , isolation of vasoactive intestinal polypeptide hormone ( which may aid the diagnosis of functioning
tumours of the pancreas or small bowel ) . -
pubmed
This latter step seems to be related to the binding mode of
protochlorophyllide to a
holochrome protein or to conformational changes in the
protochlorophyllide - holochrome . -
pubmed
Modulation of
acetylcholine metabolism in the hippocampal cholinergic pathway by intraseptally injected
substance P. -
pubmed
Local injection of
substance P into the medial nuclei of the septum decreased significantly the hippocampal
TRACh . -
pubmed
The incorporation of
( 3H )- uridine increases to a highly significant extent in third dorsal root
ganglia following
NGF treatment or limb amputation . -
pubmed
When
NGF treatment and limb amputation were combined , no further increase in
( 3H )- uridine incorporation was noted . -
pubmed
The possibility was raised that
prolactin exhibits a diurnal variation in sensitivity to stress which varies as a function of circulating levels of
corticosterone . -
pubmed
Electrophysiological studies show that the central nervous sympathetic activity at
rest and after central excitation is clearly reduced during anaesthesia with 2 vol%
halothane ; 70 min after
narcosis it returned to normal . -
pubmed
The consensus of studies in
non - insulin - dependent diabetes mellitus (
NIDDM ) seems to be that exercise has beneficial effects not only on
glucose metabolism and
insulin sensitivity , but also on elevated plasma lipids , blood pressure , and
obesity frequently following
NIDDM . -
pubmed
Serum
LH and
testosterone levels were significantly different only in the bilaterally castrate group , compared to control levels . -
pubmed
Both the normal and cryptorchid testes of all ages studied were capable of producing equal levels of
testosterone in vitro , both basally and with a
human chorionic gonadotropin (
hCG ) dose of 700 mIU / ml ( maximum stimulatory dose for both the normal and the 28 day cryptorchid testes ) . -
pubmed
Serum
FSH levels increase , but no changes occur in serum
LH and
testosterone levels . -
pubmed
For extraocular cases we recommend color coding the
thrombin with some
methylene blue . -
pubmed
alpha - Gal A mRNA was deficient in the mRNA preparation from the lymphoblastoid cells derived from the patient , and a faulty transcription resulting in an unstable
alpha - Gal A message was suggested in this case . -
pubmed
Urinary
albumin excretion rates , expressed as
albumin -
creatinine ratios ( ACR , mg / mmol ) were measured before and after exercise in 23 claudicants and 10 controls . -
pubmed
Furthermore , both tDNA and anti - tDNA are cleaved by
HhaI and
CfoI restriction endonucleases at two
GCG / C sites which would be in double - stranded regions ( the acceptor and
dihydrouridine stem ) , if the two molecules adopt the
tRNA cloverleaf structure . -
pubmed
In this view the
2 '- hydroxyl group , in
tRNA at least , is an auxiliary structural feature whose role is limited to fostering local interactions , which increase the stability of a given conformation . -
pubmed
The film contains
NAD ,
nitro blue tetrazolium , 3 - OHB dehydrogenase , and
diaphorase , and the surface is coated with modified biomembrane and can detect 50 - 1500 microM
3 - OHB within 2 - 3 min . -
pubmed
The data suggest that the reduction of serum
thyroxine levels after transplantation is attributable , at least in part , to inhibition of
thyrotropin secretion . -
pubmed
In addition to this , we calculated the count ratio of radioactivity of
99mTc - PYP (
CR ) in the infarcted myocardium to sternum to evaluate intracellular uptake of
calcium during reperfusion . -
pubmed
Noradrenaline caused contraction of the basilar artery , middle cerebral artery and small pial arteries by stimulation of
alpha 1 - adrenoceptors . -
pubmed
Ligation of
fibrinogen by
factor XIIIa with
dithiothreitol : mechanical properties of ligated
fibrinogen gels . -
pubmed
Guar did not reduce the excessive postprandial glycemic excursion , glycosylated
hemoglobin values , basal plasma
glucose concentrations , basal or incremental plasma
C - peptide values , or
body weight . -
pubmed
Mean
low - density lipoprotein cholesterol levels were significantly reduced ( P less than .001 ) by guar administration ( 116 + /- 23 vs. 104 + /- 19 mg / dl ) . -
pubmed
The present results indicate that 5 -
HT mechanisms in the CNS are important mediators of the analgesic action of
clonidine . -
pubmed
GABA - receptor agonists that are poor substrates for
GABA uptake (
muscimol ,
thiomuscimol ,
piperidine-4 - sulphonic acid ,
isoguvacine and
4,5,6,7 - tetrahydroisoxazolo [ 5,4 - c ] pyridine-3 - ol (
THIP ) evoked very long conductance changes that were not further prolonged by uptake inhibitors . -
pubmed
Preparation of
calcium phosphate for
protein chromatography . -
pubmed
It is concluded that the number of hippocampal
corticosterone receptor sites is a sensitive index of brain aging and effectiveness of
ORG 2766 . -
pubmed
Systemic doses of
capsaicin as low as 2.5 mg / kg depleted
substance P ( SP ) in dorsal roots plus ganglia ( DRG ) whereas a 10 - mg / kg dose depleted the peptide maximally in DRG and in the dorsal spinal cord . -
pubmed
High doses of
capsaicin had no consistent effects on levels of
cholecystokinin (
CCK ) ,
vasoactive intestinal polypeptide , or
somatostatin , although a transient decrease in
CCK levels was observed 4 days after dosing . -
pubmed
In the absence of
1,25 ( OH ) 2D3 , treatment of cultures for 20 - 24 h with 10(-5 ) and 10(-6 ) M
estradiol led to inhibition of
25 - hydroxyvitamin D3 [ 25 ( OH ) D3 ]- 1 - hydroxylase activity . -
pubmed
When the
1 - hydroxylase was suppressed and
25 ( OH ) 2D3 - 24 - hydroxylase was induced by treatment with
1,25 ( OH ) 2D3 ,
estradiol in concentrations of 10(-9 ) and 10(-5 ) M either had no effect or slightly inhibited
1,25 ( OH ) 2D3 production . -
pubmed
These results were unaltered when either
testosterone ( 10(-6 ) M ) or
insulin ( 5 micrograms / ml ) was present in the medium . -
pubmed
On this basis it was suggested that the normally low concentrations of
amino acids in the cerebrospinal fluid (
CSF ) , by comparison with those in plasma , were due to an active transport from the
CSF to the blood . -
pubmed
An
H1 histamine blocker markedly diminished the skin reaction to
insulin , and her plasma
glucose and glycosylated
hemoglobin AIc became well controlled . -
pubmed
CNTF proteins with 14
amino acid residues removed from the N - terminus were biologically active whereas the removal of 23
amino acids resulted in an inactive protein . -
pubmed
In addition , 18
amino acid residues could be removed from the C - terminus of the
CNTF protein without apparent loss of bioactivity , but further truncation at the C - terminus yielded biologically inactive proteins . -
pubmed
Brain
iron ,
transferrin and
ferritin concentrations in cytosolic and microsomal fractions of either whole brain or pons and cerebellum were then determined . -
pubmed
This study extends previous research by demonstrating that the brain responds to changes in body
iron status with a change in
transferrin concentration . -
pubmed
This study also notes that brain
ferritin decreases with decreasing body
iron status , though it was less responsive than
nonheme iron in liver . -
pubmed
Estrogen use has been reported to decrease
triglyceride and
low - density lipoprotein cholesterol (
LDL - C ) and increase
high - density lipoprotein cholesterol (
HDL - C ) . -
pubmed
In contrast to women , increased
androgen concentrations in men do not seem to be associated with increased cardiovascular risk factors , although
testosterone concentrations are associated with increased HDL - C and decreased
insulin concentrations . -
pubmed
Compared with results obtained when endotoxin was given alone , pretreatment with
polymyxin B resulted in significantly ( P & lt ; 0.05 ) lower maximal plasma
TNF and
IL-6 activities , and significantly lower rectal temperature and respiratory rate . -
pubmed
In view of the importance of
cholesterol as a risk factor of
cardiovascular disease , the present study analyzes the influence of the diet on blood
cholesterol levels in a group of 156 teen -
ager 14 to 18 . -
pubmed
GF109203X , a specific
PKC inhibitor , abrogates
anti - CD3 antibody - induced upregulation of
CD4 + T cell adhesion to B cells . -
pubmed
Studies using the nonspecific inhibitor
staurosporine have suggested that this phenomenon is dependent on
protein kinase C activation . -
pubmed
We found that the upregulation of
anti - CD3 - activated
CD4 + T cell adhesion was inhibited strongly and in a concentration - dependent manner by
GF109203X , a compound described as a potent and selective inhibitor of
PKC . -
pubmed
Comparative studies showed that
GF109203X and
staurosporine had similar inhibitory effects on the upregulation of activated
CD4 + T cell adhesion . -
pubmed
PMA - activated
CD4 + T cell adhesion was also inhibited by
GF109203X. -
pubmed
In contrast , passive enhancement of adhesion by treatment with the
CD11a - specific antibody NKI - L16 was unaffected by
GF109203X. -
pubmed
Hemolysin B (
HlyB ) is a membrane - bound transport protein composed of an
amino - terminal multiple membrane - spanning portion followed by a conserved
ATP binding sequence . -
pubmed
We have isolated transport - deficient mutants of
HlyA altered in the C - terminal signal sequence and used one of these , a deletion of 29
amino acids , to select compensatory mutants in the transporter protein
HlyB. -
pubmed
Fifteen mutants located at six different sites , all mapping within the
amino - terminal multiple membrane - spanning domain of
HlyB , were identified . -
pubmed
The
diadenosine polyphosphates Ap3A and
Ap4A and
adenosine triphosphate interact with
granulocyte - macrophage colony - stimulating factor to delay neutrophil apoptosis : implications for neutrophil : platelet interactions during
inflammation . -
pubmed
Adenosine triphosphate (
ATP ) and the
diadenosine polyphosphates Ap4A and
AP3A were almost as effective as
GM - CSF in delaying neutrophil apoptosis . -
pubmed
The
nucleotides could thus preserve cellular morphology , protect against chromatin fragmentation , and preserve functions such as
NADPH oxidase activity and expression of
CD16 . -
pubmed
Moreover , addition of
ATP ,
AP3A and
AP4A together with
GM - CSF resulted in more pronounced protection from apoptosis than was observed during incubation with either the
cytokine or the
nucleotides alone . -
pubmed
Because
ATP , Ap3A , and
AP4A may be secreted from activated platelets , these observations suggest that platelet - derived products , perhaps acting in combination with
endothelial - derived or immune cell - derived cytokines , can regulate neutrophil function during certain types of
inflammation . -
pubmed
Use dependence of
tetrodotoxin block of
sodium channels : a revival of the trapped - ion mechanism . -
pubmed
Nitric oxide (
NO ) , synthesized by
nitric oxide synthase (
NOS ) , has negative inotropic and cytotoxic effects on cardiomyocytes . -
pubmed
Expression of
iNOS leads to excessive production of
NO in the myocardium and may modulate cardiac contractility and ventricular morphology . -
pubmed
CRT was found to be predominantly in the sera of
SLE patients associated with immune complexes and
C1q , and only bound to the surfaces of neutrophils in the presence of low levels of
calcium and
magnesium . -
pubmed
In the retinal
pigment epithelium , choroid plexus , adrenal gland and tongue , expression begins at
E12 and in the testis and the adenohypophysis at E13 . -
pubmed
In the present study , a wide range of single doses of
trovafloxacin (
TR ) and twice - daily doses of
ciprofloxacin ( CI ) were mimicked in an in vitro dynamic model . -
pubmed
We show in this report that
NPI2 , a second gene required for
NH4 + -induced down - regulation of
Gap1p , codes for the ubiquitin hydrolase
Doa4p /
Ubp4p /
Ssv7p and that
NH4 + -induced
Gap1p ubiquitination is strongly reduced in
npi2 cells . -
pubmed
The
npi2 mutation results in substitution of an aromatic
amino acid located in a 33 - residue sequence shared by some
ubiquitin hydrolases of the
Ubp family . -
pubmed
In
proline - grown wild - type and
npi2 /
doa4 cells overproducing
ubiquitin ,
Gap1p appears to be mono - ubiquitinated at two
lysine acceptor sites . -
pubmed
Addition of
NH4 + triggers rapid poly - ubiquitination of
Gap1p , the poly - ubiquitin chains being specifically formed by linkage through the
lysine 63 residue of
ubiquitin . -
pubmed
Gap1p is thus ubiquitinated differently from the proteins targeted by ubiquitination for proteolysis by the proteasome , but in the same manner as the
uracil permease , also subject to
ubiquitin - dependent endocytosis . -
pubmed
When poly - ubiquitination through
Lys63 is blocked , the
Gap1p permease still undergoes
NH4 + -induced down - regulation , but to a lesser extent . -
pubmed
Here we report the effects of
levcromakalim (
LCRK , a channel opener ) and
glibenclamide (
GBC , a blocker ) on membrane potential , whole - cell current and the cytoplasmic
Ca2 + concentration of
renin - secreting cells ( RSC ) . -
pubmed
As monitored with the fluorescent
oxonol dye
DiBAC4 ( 3 ) ,
LCRK ( 0.3 and 1 microM ) induced a hyperpolarization of approximately 15 mV which was abolished by
GBC ( 1 microM ) . -
pubmed
Application of
GBC ( 1 microM ) produced no effect ; however , when applied after
LCRK ( 300 nM ) ,
GBC inhibited the opener - induced hyperpolarizing current with an IC50 of 103 + /- 36 nM. -
pubmed
LCRK ( 0.3 and 1 microM ) did not significantly affect the cytoplasmic
Ca2 + concentration either at
rest or after stimulation by
angiotensin II . -
pubmed
The stimulatory effect of KATP channel opening on
renin secretion is not mediated by a decrease in intracellular
Ca2 + concentration . -
pubmed
Kinetics of
synaptotagmin responses to
Ca2 + and assembly with the core SNARE complex onto membranes . -
pubmed
The synaptic vesicle protein
synaptotagmin I binds
Ca2 + and is required for efficient neurotransmitter release . -
pubmed
Here , we measure the response time of the
C2 domains of
synaptotagmin to determine whether
synaptotagmin is fast enough to function as a
Ca2 + sensor for rapid exocytosis . -
pubmed
We report that
synaptotagmin is " tuned " to sense
Ca2 + concentrations that trigger neuronal exocytosis . -
pubmed
We further demonstrate that
Ca2 + triggers penetration of
synaptotagmin into membranes and simultaneously drives assembly of
synaptotagmin onto the base of the ternary SNARE ( soluble
N - ethylmaleimide - sensitive fusion protein [
NSF ] attachment receptor ) complex , near the transmembrane anchor of
syntaxin . -
pubmed
New chemotherapeutic agents , such as
taxanes (
paclitaxel ,
docetaxel ) ,
topoisomerase inhibitors (
topotecan ,
irinotecan ) , and novel analogs (
gemcitabine ,
vinorelbine ) , may offer the promise of improved outcome , but have not yet been tested in phase III trials . -
pubmed
Molecular therapeutics , such as gene therapy , drugs that target specific oncogene activation ( such as
Ki - ras inactivation by
farnesyl transferase inhibitors ) , and hypoxic cell toxins ( such as
tirapazamine ) , are in clinical trials . -
pubmed
The survival effect of high concentration
Ang2 was blocked by pre - treatment with soluble
Tie2 receptor and the
PI 3 '- kinase - specific inhibitors ,
wortmannin and
LY294002 . -
pubmed
Accordingly , 800 ng / ml of
Ang2 induced phosphorylation of
Tie2 , the
p85 subunit of
phosphatidylinositol 3 '- kinase (
PI 3 '- kinase ) , and
serine - threonine kinase Akt at
Ser473 in the human umbilical vein endothelial cells ; lower concentrations of
Ang2 ( 50 - 400 ng / ml ) did not produce notable effects . -
pubmed
Ascorbic acid and
D - gluconic acid lactone inhibited the hydrolysis of
p - nitrophenyl beta - glucoside , but did not affect
octyl - and octylthioglucoside hydrolase activity . -
pubmed
Besides
OTG , various other
thioglucosides were hydrolysed by the novel
thioglucosidase , with almost the same activities regardless of the nature of the aglycone , including the
myrosinase model substrate
sinigrin ( a
glucosinolate ) . -
pubmed
We used Brownian dynamics techniques to compute the diffusion limited association rate constants between the negatively charged
phosphate ligand and several open forms of
PBP ( wild - type and several mutants based on an x - ray structure of open - form
PBP , mutant T141D ) . -
pubmed
Oligonucleotide fishing for
STAT6 : cross - talk between
IL-4 and
chemokines . -
pubmed
Also the
tyrosine - kinase inhibitor
herbimycin inhibited
fibrinogen mediated secretion of
chemokines . -
pubmed
The anxiolytic drugs
diazepam and
buspirone ( 1 and 5 mg / kg ) suppressed vocalization in the intertrial and
SS periods . -
pubmed
Anxiogenic
pentyletetrazole ( 1 and 5 mg / kg ) enhanced intertrial vocalization , but did not affect its reoccurrence during the
SS . -
pubmed
Anxiogenic
FG7142 ( 5 mg / kg ) did not affect intertrial vocalization , but blocked its reappearance on the
SS . -
pubmed
Quantitative assessment of contaminating
tumor cells in autologous peripheral blood stem cells of
B - cell non - Hodgkin lymphomas using immunoglobulin heavy chain gene allele - specific
oligonucleotide real - time quantitative -
polymerase chain reaction . -
pubmed
Here we demonstrate that
CT also promotes the generation of regulatory T ( Tr ) cells against bystander
Ag . -
pubmed
Furthermore , adoptive transfer of bone marrow - derived dendritic cells ( DC ) incubated with
Ag and
CT induced T cells that secreted
IL-4 and
IL-10 and low concentrations of
IL-5 . -
pubmed
Acivicin - induced alterations in renal and hepatic
glutathione concentrations and in
gamma - glutamyltransferase activities . -
pubmed
gamma - Glutamyltransferase (
gamma - GT ) catalyzes the hydrolysis of
glutathione ,
glutathione S - conjugates , and
gamma - substituted l - glutamate derivatives . -
pubmed
Acivicin is an irreversible inhibitor of
gamma - GT that has been used to study the role of gamma -
GT in
glutathione homeostasis and
glutathione - dependent bioactivation reactions . -
pubmed
Hence , diurnal and
acivicin - induced changes in renal and hepatic
glutathione concentrations along with renal and hepatic
gamma - GT activities were investigated . -
pubmed
Renal
gamma - GT activities decreased within 2 hr after giving
acivicin and remained decreased for 24 hr . -
pubmed
Molecular mechanisms of
TNF - alpha - induced
ceramide formation in
human glioma cells :
P53 - mediated oxidant stress - dependent and -independent pathways . -
pubmed
The present study was designed to examine the roles of
p53 , reactive
oxygen species ( ROS ) , and
ceramide , and to determine their mutual relationships during
tumor necrosis factor ( TNF )- alpha - induced apoptosis of
human glioma cells . -
pubmed
In cells possessing wild - type
p53 ,
TNF - alpha stimulated
ceramide formation via the activation of both neutral and
acid sphingomyelinases (
SMases ) , accompanied by
superoxide anion (
O2 - * ) production , and induced mitochondrial depolarization and
cytochrome c release , whereas
p53 - deficient cells were partially resistant to
TNF - alpha and lacked
O2 - * generation and neutral
SMase activation . -
pubmed
Restoration of functional
p53 sensitized
glioma cells expressing mutant
p53 to
TNF - alpha by accumulation of
O2 - * . -
pubmed
z - IETD - fmk (
benzyloxycarbonyl - Ile - Glu - Thr - Asp fluoromethyl ketone ) , but not
z - DEVD - fmk (
benzyloxycarbonyl - Asp - Glu - Val - Asp fluoromethyl ketone ) , blocked
TNF - alpha - induced
ceramide formation through both
SMases as well as
O2 - * generation . -
pubmed
Two separate signaling cascades ,
p53 - mediated ROS - dependent and -independent pathways , both of which are initiated by
caspase-8 activation , thus contribute to
ceramide formation in
TNF - alpha - induced apoptosis of
human glioma cells . -
pubmed
A combination of
CSF tau and
MHPG discriminated
PD from
MSA ( adjusted odds ratios : tau , 27.2 ; MHPG , 0.14 ) . -
pubmed
Ethanol inhibits palmitoylation of
G protein G alpha ( s ) . -
pubmed
Ethanol - induced alterations in neuronal expression of
G proteins G ( s ) and
G ( i ) have been invoked as a cause of heterologous desensitization . -
pubmed
However , effects of
ethanol on
G protein expression vary considerably among different experimental protocols , various brain regions and diverse neuronal cell types . -
pubmed
We studied the effect of
ethanol on
G alpha ( s ) palmitoylation . -
pubmed
In summary , the findings identify a novel effect of
ethanol on
post - translational lipid modification of
G alpha ( s ) , and represent a mechanism by which
ethanol might affect
adenylyl cyclase activity . -
pubmed
Evidence indicates that both the
Rho / Rho kinase signaling pathway and reactive
oxygen species ( ROS ) such as
superoxide and
H ( 2 ) O ( 2 ) are involved in the pathogenesis of
hypertension . -
pubmed
The antioxidants
tempol ( 3 mM ) and
catalase ( 1,200 U / ml ) or the
XO inhibitor
allopurinol ( 400 microM ) significantly reduced X / XO - induced contraction . -
pubmed
A
Rho kinase inhibitor ,
( + ) -( R )- trans-4 -( 1 - aminoethyl - N-4 - pyridil ) cyclohexanecarboxamide dihydrochloride (
Y-27632 ) , decreased the contraction in a concentration - dependent manner ; however , the
Ca ( 2 +)-independent
protein kinase C inhibitor
rottlerin did not have an effect on X / XO - induced contraction . -
pubmed
Phosphorylation of the
myosin light chain phosphatase target subunit (
MYPT1 ) was increased by ROS , and preincubation with
Y-27632 blocked this increased phosphorylation . -
pubmed
These observations demonstrate that ROS - induced
Ca ( 2 + ) sensitization is through activation of
Rho and a subsequent increase in
Rho kinase activity but not
Ca ( 2 +)-independent
PKC . -
pubmed
CYP7B1 is the enzyme responsible for hydroxylation and termination of the estrogenic actions of the
androgen metabolite ,
5alpha - androstane-3beta , 17beta - diol (
3betaAdiol ) . -
pubmed
3betaAdiol is estrogenic in
ERalpha or
ERbeta positive cells only if they do not express
CYP7B1 . -
pubmed
We conclude that , by cell - specific regulation of the estrogenicity of
3betaAdiol ,
CYP7B1 performs two major tasks : ( i ) it allows
3betaAdiol to have growth inhibitory effects through
ERbeta and ( ii ) it permits
estradiol - specific activation of
estrogen receptors by protection of certain cells from the estrogenic effects of
3betaAdiol . -
pubmed
When
CYP7B1 is inactivated ,
3betaAdiol activates
estrogen receptors indiscriminately , and the overall effect is prolonged and inappropriate exposure to
estrogen . -
pubmed
The effects of these compounds on the release of
adenine nucleotides , proteins , and
beta - N - acetyl - glycosaminidase ( a marker of lysosomal secretion ) from blood platelets activated by
thrombin were also studied . -
pubmed
Here we found that most
B - cell malignancies except
plasmacytoma respond to
CpG oligodeoxynucleotides by up - regulating expression of
costimulatory and antigen - presenting molecules , by increasing expression of
CD20 , and by proliferation . -
pubmed
Reactive
oxygen species retain a key role during both growth factor and
integrin receptor signaling , and these second messengers are recognized to be a synergistic point of confluence for anchorage - dependent growth signaling . -
pubmed
Conversely , oxidation of
PTKs leads to their activation , either by direct
SH modification or , indirectly , by concomitant inhibition of
PTPs that leads to sustained activation of
PTKs . -
pubmed
The
GSTP1 gene displays a polymorphism at codon 105 resulting in an
Ile to
Val substitution , which alters the enzymatic activity of the protein , and this has been suggested as a putative high - risk genotype in various
cancers . -
pubmed
Enhanced
post - denitrification without addition of an external
carbon source in membrane bioreactors . -
pubmed
This study investigates a
post - denitrification process without the addition of an external
carbon source combined with an enhanced biological
phosphorus removal ( EBPR ) in a membrane bioreactor (
MBR ) . -
pubmed
It was shown that even without dosing of an external
carbon source , denitrification rates ( DNR ) much above endogenous rates could be obtained in
post - denitrification systems . -
pubmed
Given these surprising results , the project team decided to identify the
carbon source used by the microorganisms in the
post - denitrification process . -
pubmed
The following hypothesis was proposed to explain the observations : the glycogen , internally stored by the substrate accumulating bacteria , if anaerobic conditions are followed by aerobic conditions could act as
carbon source for denitrification in
post - denitrification system . -
pubmed
A single
amino acid substitution in
VP7 antigenic region A , at position 96 ( Asp-->Asn ) , was consistently associated with the loss of antigenic reactivity . -
pubmed
While it is undisputed that
estrogens (
1 beta - estradiol ,
E2 ) are mainly involved in skin physiology and operate as potent hair growth modulators , our knowledge about the
estrogen target cells in skin and exact signaling pathways is still very limited . -
pubmed
The current review provides an overview of
estrogen effects on hair follicle cycling , cutaneous expression of
estrogen receptors , and potential functions of
estrogens in hair biology . -
pubmed
We discuss potential target genes of
estrogen receptor - mediated signaling in the skin , explore the interplay of
estrogens with other hormones , growth factors and enzymes , and define major open questions in this intriguing and far too long neglected area of hair research . -
pubmed
Serum
calcium - independent
phospholipase A2 activity in
bipolar affective disorder . -
pubmed
However ,
calcium - independent
PLA2 activity was significantly ( p & lt ; 0.05 ) higher in patients with a history of
psychosis compared with those with no history of
psychosis ( by 55 % ) or to
HVs ( by 31 % ) . -
pubmed
Here , we first review data evaluating the neuroprotective effects of
estrogens , a selective
estrogen receptor modulator ( SERM ) , and
estrogen receptor alpha - and beta - selective ligands in animal models of focal and global
ischemia . -
pubmed
The gerbil global
ischemia model was used to evaluate the neuroprotective effects of
estrogen , SERMs , and
ERalpha- and
ERbeta - selective compounds in the hippocampus . -
pubmed
Analysis of
neurogranin mRNA , a marker of viability of hippocampal neurons , with in situ hybridization , revealed that
estrogen treatment protected the dorsal
CA1 regions not only when administered before , but also when given 1 h after occlusion . -
pubmed
Region - dependent regulation of mesoaccumbens
dopamine neurons in vivo by the constitutive activity of central
serotonin2C receptors . -
pubmed
Central
serotonin2C receptors (
5 - HT ( 2C ) Rs ) control the mesoaccumbens
dopamine ( DA ) pathway . -
pubmed
Intra - VTA injections of the selective
5 - HT ( 2C ) R antagonists
SB 242084 and/or
SB 243213 ( 0.1 - 0.5 microg/0.2 microL ) prevented the decrease in accumbal DA outflow induced by the
5 - HT ( 2C ) R agonist
Ro 60 - 0175 ( 3 mg / kg , i.p ) , but did not affect the increase in DA outflow induced by the
5 - HT ( 2C ) R inverse agonist
SB 206553 ( 5 mg / kg , i.p ) . -
pubmed
In the perfusion model , maximal absorption and metabolism of
paeoniflorin occurred in duodenum and jejunum , which were significantly decreased by a
glucosidase inhibitor
gluconolactone ( 20 mM ) . -
pubmed
In contrast ,
MRP inhibitors
MK-571 and
leukotriene C4 did not affect transport of
paeoniflorin . -
pubmed
Lastly ,
sinomenine was also shown to significantly increase the absorptive transport of
digoxin ( a prototypical
p - glycoprotein substrate ) and to significantly decrease its secretory transport . -
pubmed
CONCLUSIONS : Poor permeation , p - gp - mediated efflux , and hydrolysis via a
glucosidase contributed to the poor bioavailability of
paeoniflorin . -
pubmed
Sinomenine ( an inhibitor of the
p - gp - mediated
digoxin efflux ) increased
paeoniflorin 's bioavailability via the inhibition of
p - gp - mediated
paeoniflorin efflux in the intestine . -
pubmed
BaP induces
COX-2 expression , and an interaction between
BaP and
estrogen in relation to
COX-2 expression is suspected . -
pubmed
However , co - treatment with
BaP and E(2 ) potentiated
COX-2 mRNA expression and significantly elevated
PGE2 secretion . -
pubmed
Utilizing specific inhibitors and reporter assays , we further investigated the potentiation mechanisms of E(2 ) on
BaP - induced
COX-2 expression . -
pubmed
First , E(2 ) activated
estrogen receptor to increase
PGE2 secretion , which directly increased
COX-2 expression . -
pubmed
Second , E(2 ) potentiated
BaP - induced
nuclear factor - kappaB (
NF - kappaB ) activation , which regulates
COX-2 expression . -
pubmed
Third , although the
aryl hydrocarbon receptor (
AhR ) did not play a role in
BaP - induced
COX-2 expression , the potentiation effect of E(2 ) itself was
AhR dependent . -
pubmed
We further demonstrated that
BaP induced the production of genotoxic E(2 ) metabolites (
2 - and 4 - hydroxyestradiols ) via
AhR - up - regulated
cytochromes P450 1A1 and 1B1 . -
pubmed
Our findings that the
BaP - E ( 2 ) interaction enhanced
COX-2 expression and
hydroxyestradiol accumulation in the media of cultivated lung cells and tissues provide the needed scientific basis for higher risk of
BaP - associated
lung cancer in females . -
pubmed
On the other hand , blood
glucose level , serum
NO ,
malondialdehyde (
MDA ) , a marker of lipid peroxidation , protein oxidation indices including protein
carbonyl ( PCO ) and
advanced oxidation protein products ( AOPP ) were significantly elevated in pancreas of the
diabetic group . -
pubmed
CONCLUSIONS : A significant reduction in
PD occurred in two of the three groups with
alpha-1 adrenergic blockers (
tamsulosin and
doxazosin ) , but this effect was not sustained at 30 days . -
pubmed
The polymorphisms Asn130Asp and Val174Ala in
OATP1B1 and the
CYP2C9 allele * 3 independently affect
torsemide pharmacokinetics and pharmacodynamics . -
pubmed
The IC(50 ) ( the concentration causing 50 % inhibition of cell growth ) of
adriamycin (
ADR ) were assayed by
MTT method , the expression of
MDR1 mRNA was measured by RT - PCR , the concentration of
p - glycoprotein (
P - gp ) and intracellular
ADR were detected by flow cytometry . -
pubmed
The following studies were performed : measurement of
( 14 ) C - labelled
telavancin cellular uptake and subcellular distribution ( cell fractionation ) , determination of pericellular membrane integrity (
lactate dehydrogenase release ) , electron microscopy with morphometric analysis of changes in lysosome size and determination of total phospholipid and
cholesterol content . -
pubmed
Telavancin subcellular distribution was superimposable on that of a lysosomal marker (
N - acetyl - beta - hexosaminidase ) . -
pubmed
It did not cause an increase in the release of
lactate dehydrogenase and did not induce significant increases in total phospholipid or
cholesterol content . -
pubmed
Commercially available microbial biopolymers of xanthan gum and guar gum were used to encapsulate
CRT wastes , reducing
Pb leachability as measured by standard USEPA methods . -
pubmed
Determinants of
cytochrome P450 2C8 substrate binding : structures of complexes with
montelukast ,
troglitazone ,
felodipine , and
9 - cis - retinoic acid . -
pubmed
X - ray crystal structures of
P450 2C8 complexed with
montelukast ( 2.8 A ) ,
troglitazone ( 2.7 A ) ,
felodipine ( 2.3 A ) , and
9 - cis - retinoic acid ( 2.6 A ) were determined to examine ligand - protein interactions for these chemically diverse compounds . -
pubmed
The structure of the
9 - cis - retinoic acid complex reveals that two substrate molecules bind simultaneously in the active site of
P450 2C8 . -
pubmed
In conclusion ,
caffeine produced useful numbers of seeds for all clones ;
gliadin banding patterns and chromosome counts indicated that genetic and cytogenetic stability of the doubling process were equal or superior for
caffeine compared with
colchicine . -
pubmed
Important aspects of bone mineral density (
BMD ) regulation are endocrine systems , notably hormonal regulation of adrenal
corticoids , as indicated by clinical knowledge of glucocorticoid - induced
osteoporosis . -
pubmed
We studied single
nucleotide polymorphisms ( SNPs ) in genes encoding
thrombospondin , type 1 , domain - containing 4 and 7A (
THSD4 and
THSD7A ) for possible association with lumbar and femoral BMD among 337 Japanese women with
osteoporosis who participated in the BioBank Japan project . -
pubmed
The transcription factor
DeltaFosB accumulates in the denervated striatum and dimerizes primarily with
JunD upon repeated
L-3,4 - dihydroxyphenylalanine (
L - DOPA ) administration . -
pubmed
Low
Mannan - binding lectin serum levels are associated with complicated
Crohn ' s disease and reactivity to
oligomannan ( ASCA ) . -
pubmed
Natural polysaccharides such as amylose (
AMY ) ,
alginate sodium ( ALG ) , and chitosan ( CHI ) have been noncovalently wrapped onto single - wall
carbon nanotubes ( SWCNTs ) to give a series of SWCNT scaffolds , termed as AMY - SWCNT , ALG - SWCNT , CHI - SWCNT , and CHI / ALG - SWCNT scaffolds . -
pubmed
Considering all properties , the relatively electrical neutral and hydrophilic
AMY - SWCNT scaffolds bearing only -
OH groups are able to sustain the highest cell viability after 72 h culturing . -
pubmed
True
catalases are
tyrosine - liganded , usually tetrameric , hemoproteins with subunit sizes of approximately 55 - 84 kDa . -
pubmed
paratuberculosis ( annotated as
MAP-2744c ) , that retains the core elements of the
catalase fold and exhibits an organic
peroxide - dependent
peroxidase activity . -
pubmed
MAP-2744c exhibits negligible catalatic activity , weak peroxidatic activity using
hydrogen peroxide ( 20 / s ) and strong
peroxidase activity ( approximately 300 / s ) using organic
hydroperoxides as co - substrate . -
pubmed
Key
amino acid differences significantly impact prosthetic group conformation and placement and confer a distinct activity to this prototypical member of a group of conserved bacterial "
minicatalases " . -
pubmed
Its structural features and the result of the enzyme assays support a role for
MAP-2744c and its close homologues in mitigating challenge by a variety of reactive
oxygen species . -
pubmed
This clay was characterized according to the following techniques : Energy Dispersive Spectroscopy ( EDS ) , Thermogravimetry (
TG ) , Differential Scanning Calorimetry ( DSC ) , X - ray Diffraction ( XRD ) , Fourier Transformed Infrared Spectroscopy ( FTIR ) , Physisorption of
N ( 2 ) ( BET ) ,
Helium Picnometry and Scanning Electron Microscope ( SEM ) with metal mapping . -
pubmed
Breed differences for serum
leptin ,
creatinine , and
cholesterol were found throughout the study with AG being greater than RO . -
pubmed
Although there were no breed differences ( P = 0.21 ) at
TN , only AG steers exhibited a HS - induced increase ( P & lt ; 0.05 ) in
prolactin ,
creatinine , and
cholesterol concentrations to suggest that an increase in rectal temperature is required for this effect . -
pubmed
The aim of our studies was to determine activity of these
enzymes after induction by
benzoic acids in cometabolic systems with
nitrophenols . -
pubmed
From cometabolic systems with
nitrophenols as cometabolites and
3,4 - dihydroxybenzoate as a growth substrate ,
dioxygenases with the highest activity of
protocatechuate 3,4 - dioxygenase were isolated . -
pubmed
Catechol 2,3 - dioxygenase was active only in cultures with
4 - nitrophenol . -
pubmed
Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas - chromatography coupled to a flame ionization detector (
GC - FID ) . -
pubmed
The aim of this study was to develop , validate and apply a methodology based on gas chromatography coupled to optical fiber detection (
GC - OF ) for screening five
fatty acids used as indicators of fungal and bacterial communities in urban soils . -
pubmed
Additionally , the
GC -
OF showed a great potential as alternative for determination of eleven or more
fatty acids in urban soils . -
pubmed
DrcA is related to
glutathione S - transferases , but with a key
asparagine - to -
cysteine substitution in the catalytic pocket . -
pubmed
Mercury induces the expression of
cyclooxygenase-2 and
inducible nitric oxide synthase . -
pubmed
Here , we report biochemical evidence that
mercury alone induces
NF - kappaB activation , resulting in the induced expression of
COX-2 and
iNOS . -
pubmed
GC -
MS of amaryllidaceous
galanthamine - type alkaloids . -
pubmed
In the present study , we focused on
imipramine , a widely used antidepressant , and investigated whether it might protect against the neuronal cell
death induced by
tunicamycin , an
ER stress inducer . -
pubmed
Interestingly , in such cells application of
imipramine normalized the expression of the
sigma-1 receptor , which was decreased by treatment with
tunicamycin alone . -
pubmed
Additionally ,
NE-100 , a selective
sigma-1 receptor antagonist , abolished the protective effect of
imipramine against such
tunicamycin - induced cell
death . -
pubmed
These findings suggest that
imipramine protects against
ER stress - induced hippocampal neuronal cell
death both in vitro and in vivo . -
pubmed
Large scale preparation of
midkine antisense
oligonucleotides nanoliposomes by a cross - flow injection technique combined with ultrafiltration and high - pressure extrusion procedures . -
pubmed
The
midkine antisense
oligonucleotide (
MK - ASODN , 5'-CCC CGG GCC GCC CTT CTT CA-3 ' ) nanoliposomes have been identified to suppress
hepatocellular carcinoma (
HCC ) growth effectively , and have a great potential to be an effective target drug for
HCC . -
pubmed
Enzymatic loss in
alkaptonuria (
AKU ) , an
autosomal recessive disorder , is caused by mutations in the
homogentisate 1,2 dioxygenase (
HGD ) gene , which decrease or completely inactivate the function of the
HGD protein to metabolize
homogentisic acid (
HGA ) . -
pubmed
Two new
platinum ( II ) complexes ,
trans -[ Pt ( 2 - mpy )( 2 ) ] 4H ( 2 ) O ( 1 ) and
[ PtCl ( 2 - pyc )( 2 - hmpy ) ] H ( 2 ) O ( 2 ) , where 2 - hmpy=
2 -( hydroxymethyl ) pyridine , 2 -
mpy = deprotonated
2 - hmpy and 2 - pyc =
pyridine-2 - carboxylate , have been synthesized and characterized by elemental analysis , IR , NMR , and X - ray crystallography . -
pubmed
3 - Hydroxypyridin-2 - thione as novel
zinc binding group for selective
histone deacetylase inhibition . -
pubmed
Small molecules bearing
hydroxamic acid as the
zinc binding group ( ZBG ) have been the most effective
histone deacetylase inhibitors ( HDACi ) to date . -
pubmed
We have identified
3 - hydroxypyridin-2 - thione (
3 - HPT ) as a novel
ZBG that is compatible with
HDAC inhibition . -
pubmed
Remarkably ,
3 - HPT gives no inhibition of
HDAC 1 . -
pubmed
Subsequent optimization led to several novel
3HPT - based HDACi that are selective for
HDAC 6 and
HDAC 8 . -
pubmed
The discovery of the ribosomal origin of
thiopeptides revealed a unifying theme , showing that the structural complexity arises from
post - translational modifications ( PTMs ) of precursor peptides . -
pubmed
Utilizing
nosiheptide biosynthesis as a model system , we herein consider how nature coordinates a number of highly interwined , common and specific
PTMs to accomplish the complexity of ribosomally synthesized and
post - translationally modified peptides . -
pubmed
After adjustment of age , sex , and
BMI ,
FGF21 was positively correlated with total
cholesterol ( P & lt ; 0.05 ) and
triglyceride ( P & lt ; 0.01 ) . -
pubmed
Cisplatin - induced downregulation of
SOX1 increases drug resistance by activating autophagy in
non - small cell lung cancer cell . -
pubmed
Long - term
cisplatin exposure promotes methylation of
SOX1 in
ovarian cancer cell , suggesting that
SOX1 may be involved in
cisplatin resistance . -
pubmed
Our aim was to test the hypothesis that
cisplatin resistance is associated with alteration of
SOX1 expression in
NSCLC . -
pubmed
Expression of levels of
SOX1 was examined using RT - PCR in
cisplatin resistance cells and parental cells . -
pubmed
The level of
SOX1 mRNA in
cisplatin resistance cells was markedly reduced when compared to parental cells . -
pubmed
Promoter methylation of
SOX1 was induced in
cisplatin resistance cells . -
pubmed
We also found that
SOX1 silencing enhanced the
cisplatin - mediated autophagy in
NSCLC . -
pubmed
The footprint periphery , including filopodia , undergoes extensive
F - actin remodeling , an effect abolished by the
actomyosin inhibitors
cytochalasin D and
blebbistatin . -
pubmed
Isoproterenol induces vascular oxidative stress and
endothelial dysfunction via a
Gialpha - coupled
beta2 - adrenoceptor signaling pathway . -
pubmed
CONCLUSIONS : The
beta2 - adrenoceptor / Gialpha signaling pathway is implicated in the enhanced vasoconstrictor response and
eNOS uncoupling - mediated oxidative stress due to
ISO treatment . -
pubmed
Mlh1 -
Mlh2 foci were similar to
Mlh1 -
Pms1 foci : they required mispair recognition by
Msh2 -
Msh6 , increased in response to increased mispairs or downstream defects in
MMR , and formed after induction of DNA damage by
phleomycin but not double - stranded breaks by
I - SceI. -
pubmed
No particular
p53 genotype at exon 4 of codon 72 was found to be associated with
oral leukoplakia , but " C " allele (
proline ) at exon 4 of codon 72 was significantly raised in these patients . -
pubmed
RESULTS : Participants with pre - transition HbA1c & lt;6.5 % were older with shorter duration
diabetes and took less
insulin but more non -
insulin glucose - lowering agents than those with higher HbA1c . -
pubmed
A total of 823 participants achieved a final HbA1c & lt;6.5 % , and had greater
post - transition reductions in
BMI ,
insulin dose and secretagogue and
acarbose use than those with higher HbA1c ( p & lt ; 0.0001 ) . -
pubmed
HbA1c & lt;6.5 % at transition predicted final HbA1c & lt;6.5 % ( crude RR 4.9 [ 95 % CI 4.0 , 5.9 ] ; RR 3.9 [ 95 % CI 3.2 , 4.8 ] adjusted for demographics , co - interventions , pre - intervention HbA1c ,
BMI and
glucose - lowering medication , and
post - transition change in both
BMI and
glucose - lowering medication ) . -
pubmed
By means of intracellular microelectrode recordings we found that
ANG II reduced gCl in the nanomolar range and in a concentration - dependent manner ( EC50 = 0.06 muM ) meanwhile increasing
potassium conductance ( gK ) . -
pubmed
Both effects were inhibited by the
ANG II receptors type 1 ( AT1 )- receptor antagonist
losartan and the
protein kinase C inhibitor
chelerythrine ; no antagonism was observed with the
AT2 antagonist
PD123,319 . -
pubmed
The scavenger of reactive
oxygen species ( ROS )
N - acetyl cysteine and the
NADPH - oxidase (
NOX ) inhibitor
apocynin also antagonized
ANG II effects on resting ionic conductances ; the
ANG II - dependent
gK increase was blocked by
iberiotoxin , an inhibitor of
calcium - activated potassium channels . -
pubmed
Both
ANG II and the
AT1 agonist
L162,313 increased the intracellular
calcium transients , measured by
fura-2 , with a two - step pattern . -
pubmed
These latter effects were not observed in the presence of
losartan and of the
phospholipase C inhibitor
U73122 and the in absence of extracellular
calcium , disclosing a
Gq - mediated
calcium entry mechanism . -
pubmed
The data show for the first time that the
AT1 - mediated
ANG II pathway , also involving
NOX and ROS , directly modulates ion channels and
calcium homeostasis in adult myofibers . -
pubmed
Predicting scleral
GP lens entrapped tear layer
oxygen tensions . -
pubmed
Oxygen delivery to the cornea in the presence of a contact lens system continues to be a subject of interest , and scleral
GP lenses are no exception . -
pubmed
This paper utilizes an existing model based on simultaneous two lens systems ( piggyback lenses ) as a resistance to
oxygen in series , and applies this model to scleral
GP lens systems . -
pubmed
Our results may have important implications in host defense , especially the immunopotentiation function of
OBP on the
cyclophosphamide - induced
epithelial cell injury , as well as
intestinal disorders involving
inflammation and
infection . -
pubmed
Conflicting evidence suggests that administration of
allopurinol , a
xanthine oxidase inhibitor can prevent
CIN . -
pubmed
Malondialdehyde (
MDA ) and
glutathione (
GSH ) levels and
catalase (
CAT ) and
superoxide dismutase (
SOD ) activities in the tissues were determined . -
pubmed
The activity of
CAT increased only in the kidney tissue of the
nebivolol+
hHcy group ( P<0.01 ) , and the activity of
SOD was significantly increased in all the tissues in this group . -
pubmed
RESULTS : Effects of
PKC activators ,
phorbol-12,13 - dibutyrate (
PDBu ) , and
phorbol-12,13 - myristate (
PMA ) , were concentration - dependent , with high concentrations increasing frequency of micturition , and sensitivity of intramural nerves to electrical field stimulation (
EFS ) , in vitro , while lower concentrations had no effect on BMS sensitivity to
EFS . -
pubmed
The
PKC inhibitors ,
bisindolylmaleimide1 ( Bim-1 ) , ( 28 nM ) , and
Ro318220 ( 50 muM ) triggered an increase in the number of non - voiding contractions ( NVC ) , and a decrease in the voided volume associated with reduced ability to maintain contractile force upon
EFS , but did not affect peak force in vitro . -
pubmed
Application of a low concentration of
PDBu inhibited spontaneous contractions , in vitro , and
Bim-1 - induced NVC , and restored normal voiding frequency during urodynamic recordings in vivo . -
pubmed
Tissue reactivity of both Cultrex coated and uncoated
glucose sensors was evaluated at 7 , 14 , 21 and 28 days
post - sensor implantation with standard histological techniques . -
pubmed
Bioanalysis of
emixustat (
ACU-4429 ) in whole blood collected with volumetric absorptive microsampling by LC -
MS /
MS . -
pubmed
VC collected at birth from 156 newborns within the ALADDIN birth cohort was analyzed and 3 different groups of lipid mediators (
eicosanoids and related
oxylipin analogs ,
endocannabinoids and
sphingolipids ) were screened using LC -
MS /
MS . -
pubmed
Somatostatin receptor ligands ( SRLs ) were the most common drug class used first line ( 57.2 % ) ;
cabergoline ( 27.8 % ) was the most common treatment , followed by
octreotide LAR ( 22.3 % ) and
lanreotide ( 19.7 % ) . -
pubmed
Investigation of selective
JAK1 inhibitor
GSK2586184 for the treatment of
psoriasis in a randomized placebo - controlled phase IIa study . -
pubmed
BACKGROUND :
GSK2586184 is a selective oral
Janus kinase ( JAK ) 1 inhibitor being evaluated as a treatment for moderate - to - severe
plaque - type psoriasis . -
pubmed
The maximum
xylanase activity of optimized (
xynB -
opt ) gene was 33500 U / mL after
methanol induction for 144 h in 50 L bioreactor , which was 59 % higher than that by wild - type (
xynB ) gene . -
pubmed
Effects of academic exam stress on nasal
leukotriene B4 and
vascular endothelial growth factor in
asthma and health . -
pubmed
We determined
LTB4 and
VEGF concentrations using Enzyme - Linked Immunoassays . -
pubmed
Changes in nasal
LTB4 and
VEGF were not associated with salivary
cortisol , exhaled
nitric oxide , or spirometric lung function . -
pubmed
Corticosterone ,
insulin and
glucose concentrations all increased with temperature , but with different thermal response curves , suggesting that high temperatures differently affect energy - regulation pathways . -
pubmed
Involvement of the
NADPH Oxidase NOX2 - Derived Brain Oxidative Stress in an Unusual Fatal Case of
Cocaine - Related
Neurotoxicity Associated With Excited
Delirium Syndrome . -
pubmed
Here , we investigated the possible role of the
Nicotinamide Adenine Dinucleotide Phosphate oxidase NOX2 - derived brain oxidative stress in a fatal case of
cocaine - related
neurotoxicity , associated to excited
delirium syndrome . -
pubmed
These results support a crucial role of
NOX2 - derived brain oxidative stress in
cocaine - induced
brain dysfunctions and
neurotoxicity . -
pubmed
A binary logistic regression analysis was conducted to predict the presence or absence of
MAT in the treatment plan of pregnant women using the following independent variables : state prenatal child abuse law , referral source , geographical region , and Medicaid coverage of
methadone . -
pubmed
Conversion of
vinyl pyranosylamine and
vinyl furanosylamines to
2,6 - and 2,5 - disubstituted pyrrolidine and
piperidine iminosugars , respectively , in one
pot was developed using Kimura and Tamaru 's procedure , where a
Pd salt in the presence of
Et2Zn was used for the domino reaction . -
pubmed
Photosynthesis -
nitrogen relations in Amazonian tree species :
II . -
pubmed
Mothers / caregivers visiting health facilities obtained mainly oral rehydration salt ( ORS ) ( 39.8 % ) and home - recommended fluids (
HRF ) ( 40.3 % ) , but few of them ( 11.9 % ) obtained ORS plus
zinc supplementation . -
pubmed
Symptomatic responses did not differ significantly between regimens ; both gave
clavulanate levels sufficient to inhibit
beta - lactamase activity . -
pubmed
Different treatment approaches were observed with
corticosteroids administered in 87 % ,
cyclosporine in 49 % ,
etoposide (
VP-16 ) in 39 % , and monoclonal anti -
TNF in 6 % of cases . -
pubmed
Interestingly we observed differences in the role of molecules involved in damage sensing (
TOLL - like receptors ) , damage signalling (
MyD88 ) and immune regulation (
cytokines ,
CD73 , lymphocytes ) for the pathogenesis and progression of DNA damage - induced
pneumopathy between the models of
pneumopathy induced by whole thorax irradiation or treatment with the radiomimetic drug
BLM . -
pubmed
While
miltefosine inhibits the synthesis of
phosphatidylcholine and also affects the parasite mitochondrion , inhibiting the
cytochrome c oxidase , it is to be expected that this potent drug also produces its effect through other targets . -
pubmed
Grade
IV -
titanium threaded implants were placed at 4 weeks
post - extraction in maxillary first molars . -
pubmed
For instance , in the control group of the fluid - mechanical model , MRI - TIFP was a strong predictor of
WIN - TIFP (
R2 = 0.76 , p & lt ; .0001 ) . -
pubmed
A similar result was found in the
bevacizumab - treated group of the empirical model (
R2 = 0.93 , p = .014 ) . -
pubmed
CNO treatment induced pro - inflammatory mediator production per se from cells expressing
Gq - DREADDs , and inhibited lipopolysaccharide- and
CCL2 - induced inflammatory signaling from cells expressing
Gi - DREADDs . -
pubmed
Protective role of
rosmarinic acid on
amyloid beta 42 - induced echoic
memory decline : Implication of oxidative stress and
cholinergic impairment . -
pubmed
In the present study , we examined whether
rosmarinic acid ( RA ) reverses
amyloid beta (
Abeta ) induced reductions in antioxidant defense , lipid peroxidation , cholinergic damage as well as the central
auditory deficits . -
pubmed
Abeta42 injection led to a significant increase in the levels of
thiobarbituric acid reactive substances ( TBARS ) and
4 - Hydroxy-2 - nonenal (
4 - HNE ) but decreased the activity of antioxidant enzymes (
SOD ,
CAT ,
GSH - Px ) and
glutathione levels . -
pubmed
Moreover ,
Abeta42 injection resulted in a reduction in the
acetylcholine content and
acetylcholine esterase activity . -
pubmed
The existence of a so - called brain
renin -
angiotensin system (
RAS ) is controversial . -
pubmed
Given the presence of the blood - brain barrier ,
angiotensin generation in the brain , if occurring , should depend on local synthesis of
renin and
angiotensinogen . -
pubmed
RESULTS : When
methanol fixation protocol was broken up into three steps : fixation , storage and rehydration , we found that PBMC RNA was degraded during rehydration with
PBS , not at cell fixation and up to 3 - month storage steps . -
pubmed
Resuspension but not rehydration in 3x saline
sodium citrate ( SSC ) buffer instead of
PBS preserved PBMC RNA integrity and prevented RNA leakage . -
pubmed
Lead Optimization of
5 - Aryl Benzimidazolone- and
Oxindole - Based
AMPA Receptor Modulators Selective for
TARP gamma-8 . -
pubmed
Herein , we describe the discovery , lead optimization , and preclinical characterization of
5 - arylbenzimidazolone and
oxindole - based negative modulators of AMPARs associated with
TARP gamma-8 , the primary
TARP found in hippocampus . -
pubmed
High - throughput screen lead 4 was optimized for potency and brain penetration to provide
benzimidazolone 3 ,
JNJ-55511118.1 Replacement of the
benzimidazolone core in 3 with an
oxindole mitigated reactive metabolite formation and led to the identification of 18 (
GluA1 / gamma-8 pIC50 = 9.7 ) . -
pubmed
Urea was shown to promote the deregulation of
STL1 expression , overcoming
glucose repression . -
pubmed
The consistency between
Fps1 - mediated
ethanol secretion and
ethanol in the extracellular media reinforces previous suggestions that
ethanol might exit the cell through the
Fps1 channel . -
pubmed
The
hormone - sensitive lipase (
HSL ) and
Ser563 - pHSL levels were also significantly reduced by treatment with 5 mg / mL of
ABG extract . -
pubmed
Upon incorporation of the additives we achieve space - charge limited current characteristics that resemble molecular single crystals such as
rubrene with high , trap - free
SCLC mobilities up to 0.2 cm2 / Vs and a width of the residual tail state distribution comparable to kBT . -
pubmed
Both theoretical prediction and experimental photophysical data indicate a small
S1 -
T1 energy gap ( DeltaE ST ) and a relative large fluorescence rate ( k F ) in an
o - phenylene - bridged 2 - cyanopyrazine/
3,6 - di - tert - butylcarbazole compound (
TCzPZCN ) . -
pubmed
A sky - blue device containing a derivative of
TCzPZCN achieves an improved EQE maximum of 12.2 % by suppressing the non - radiative decay at
T1 . -
pubmed
Can
carbene decorated
[ FeFe ]- hydrogenase model complexes catalytically produce
dihydrogen ? -
pubmed
Cyclic alkyl amino carbene (
CAAC ) anchored [
FeFe]-
hydrogenase model complex featuring rotated conformation at one of the
iron centers are found to be promising candidate for effective production of
dihydrogen . -
pubmed
Our results indicated that after knockdown of
NF - kappaB , the autophagy was significantly increased and the expression of p -
AKT and p -
mTOR protein markedly decreased , but the level of autophagy was inhibited after treatment with
AKT activator
SC79 , suggesting the involvement of
AKT /
mTOR - mediated autophagy was under autophagy activation . -
pubmed
As for co - AD of two feedstocks , introduction of
SS ( 25 % , VS basis ) in FW significantly improved the process stability and archaea / total microbe ratio ( from 0.4 % to 17.1 % ) , which might be due to the regulating effect of abundant trace metals in
SS ; co - AD of
SS ( 25 % , VS basis ) with YW improved the
methane yield by 2.04 times than AD of YW only together with higher
methane contents ( 57.4 + - 1.3 % vs. 50.9 + - 2.2 % ) ; in co - AD of FW and YW , synergistic effects in terms of increased
methane production ( 3.4 - 19.1 % ) were observed , which was correlated with more robust growth of both bacteria and archaea . -
pubmed
Resection of the
thymoma treated her
myasthenia gravis , but led to an
aplastic anaemia resistant to
granulocyte - colony stimulating factor ,
cyclosporin and
horse anti - thymocyte globulin treatment . -
pubmed
Herein , a multi - arm
star - branched polymer was prepared by conjugating
oligolysine onto a rigid hyperbranched
poly ( amido amine ) core via click chemistry , followed by covalent modification with
Gd chelate and
folic acid (
FA - OLL - g - HBPAMAM - DTPA - Gd ) . -
pubmed
This multi - arm
star - branched polymer contrast agent exhibited much higher longitudinal relaxivity ( r1 = 13.34 mM-1 s-1 ) as compared to a commercial contrast agent (
Gd - DTPA , r1 = 4.2 mM-1 s-1 ) . -
pubmed
The study results indicated that compared to
PDS ,
PRP - loading
PDS exhibited the enhanced mechanical properties and released growth factors in a slow and sustained manner to supplement the loss of growth factors during decellularization . -
pubmed
When compared with
PDS ,
PRP - loading
PDS significantly promoted the colonization , proliferation and pro - angiogenic genes expression of cells on scaffolds . -
pubmed
RESULTS : The central administration of
galanin enhanced
2 - deoxy -[ 3H ]- D - glucose ,
peroxisome proliferator - activated receptor gamma and
adiponectin levels , food intake and
body weight ,
GLUT4 mRNA expression and
GLUT4 concentration in plasma membranes , as well as homeostasis model assessment -
insulin resistance index . -
pubmed
Those effects of
galanin may be blocked by M817 , and imitated by
M1145 except for food intake and
body weight . -
pubmed
Stomatal closure induced by darkness or
ABA was inhibited by the
H2O2 - scavenging enzyme
catalase or the antioxidant
N - acetyl cysteine (
NAC ) , or by
diphenylene iodonium (
DPI ) , an inhibitor of the
H2O2 - generating enzyme
NADPH oxidase . -
pubmed
Such increases were inhibited by
catalase ,
NAC or
DPI , consistent with the effects of these compounds on stomatal apertures . -
pubmed
The bladders were dissected for the tissue antioxidant enzyme activities and lipid peroxidation , including
malondialdehyde (
MDA ) ,
superoxide dismutase (
SOD ) , total antioxidant status ( TAS ) and total oxidant status ( TOS ) . -
pubmed
Moreover , we reveal that this H7N9 protein prevents the interaction between
TRAF3 and
cIAP2 that mediates
Lys48 - linked polyubiquitination of
TRAF3 for degradation . -
pubmed
During photosynthesis , an enzyme
Ribulose 1,5 bisphosphate carboxylase / oxygenase (
Rubisco ) combines its substrate
Ribulose 1,5 bisphosphate (
RuBP ) with
CO2 to produce
phosphoglycerate (
PGA ) . -
pubmed
Alongside ,
Rubisco also takes up
O2 and produce
2 - phosphoglycolate (
2 - PG ) , a toxic compound broken down into
PGA through photorespiration . -
pubmed
The
C3 model at elevated level of inorganic
phosphate ( Pi ) , exhibited a significant change in
RuBP ( + 355 % ) and
PGA ( - 98 % ) which is attributable to the low availability of
Ci . -
pubmed
Post - synthetic ' click ' synthesis of RAFT polymers with pendant self - immolative
triazoles . -
pubmed
We investigate the polymerization kinetics ,
post - synthetic modification , and self - immolation mechanism of a model polymer system , and discuss avenues for future studies on poly - pendant self - immolative
triazoles as a modular , stimuli - responsive macromolecule platform . -
pubmed
In the presence of an
ATPase (
adenosinetriphosphatase ;
ATP phosphohydrolase ;
EC 3.6.1.3 ) inhibitor
dicyclohexylcarbodiimide , pHin remained equal to pHex even in the presence of
glucose . -
pubmed
Ethanol - induced inhibition of
guanylate cyclase in liver , pancreas , stomach and intestine . -
pubmed
Ethanol decreases hepatic protein and
albumin synthesis , and inhibits pancreatic water ,
bicarbonate , and protein secretion . -
pubmed
Since these actions of
ethanol are opposite to those reported for
secretin , cholecystokinin - pancreozymin , and
pentagastrin which may be mediated through increases in
cyclic GMP , it appeared possible that the inhibitory actions of
ethanol might be mediated through inhibition of
guanylate cyclase , the enzyme that catalyzes the production of
cyclic GMP . -
pubmed
The inhibitory effects of
ethanol on the
guanylate cyclase -
cyclic GMP system of these tissues provide a possible explanation for some of the diverse effects of
ethanol on these tissues . -
pubmed
Markers of this group controlling the regulation of
riboflavin biosynthesis were mapped between markers of resistance to
acriflavin and
streptomycin (
strC group ) . -
pubmed
The value of cotransfer index between
acriflavin - resistance markers and
ribC markers was found to be 26 - -32 % . -
pubmed
Three subgroups were identified : One was unrease - positive , produced
porphyrin from
delta - aminolevulinic acid , and grew on infusion mediums supplemented only with
V factor ; the 2nd was unrease - negative ,
porphyrin - positive , and grew only on serum - enriched mediums with added
V factor ; and the 3rd was unrease - negative ,
porphyrin - negative , and grew only on serum - enriched mediums with added V and X factors . -
pubmed
Other tests , such as
beta - galactosidase ,
hemolysis , and fermentation of
carbohydrates were of secondary value in differentiating between these species . -
pubmed
The core peptide derived from
cyanogen bromide cleavage have been further digested with
trypsin and the resulting peptides have been separated on Dowex 1X2 . -
pubmed
All fragments have been characterized by their
amino acid composition , by determination of their N - terminal sequence using automatic Edman degradation and of their C - terminal sequence following the kinetics of
amino acid cleavage by
carboxypeptidases A and B. -
pubmed
In contrast to
guanethidine - treated parotid glands , no retention of
alpha - amylase was observed . -
pubmed
Level of plasma
prekallikrein and its inhibitors in reactors and nonreactors during intravenous enhancement with
contrast media . -
pubmed
No effect was seen on plasma
triglycerides ,
cholesterol ,
HDL -
cholesterol and
gamma - glutamyltranspeptidase at any stage of the trial . -
pubmed
No other plasma free essential
fatty acid changed during the trial , although the
HDL :
cholesterol increased slightly but non - significantly with an increase in
EPA and
DHA . -
pubmed
Upon sperm incubation in the presence of free
insulin , a dose - dependent release of phospholipid
phosphorus occurred , with a concomitant derangement of head cell membrane . -
pubmed
Analyses of the levels of
HMGr and
LDLr mRNAs under a variety of culture conditions that perturb intracellular
sterol metabolism , or which differ in the levels of extracellular
sterols , indicated that the
hepatoma cells and fibroblasts responded similarly in terms of the repression or induction ratios of both mRNAs . -
pubmed
A
ruthenium complex (
ICR ) had a higher activity in the resistant line . -
pubmed
In the presence of extracellular
Ca2 + ( CaDT -
PA added ) , the rise in [
Ca2 +] i was due to a combination of
Ca2 + release from internal stores and influx of extracellular
Ca2 + . -
pubmed
In the absence of extracellular
Ca2 + ,
LXA4 dose - dependently reduced the subsequent response of neutrophils to fmlp ; this is presumably because
LXA4 discharges the store , and so reduces the amount of
Ca2 + available for subsequent release by
fmlp . -
pubmed
Three of these peptides ,
vasoactive intestinal peptide ,
substance P , and
somatostatin , regulate the function of immune effector cells in gut - associated lymphoid tissue . -
pubmed
Vasoactive intestinal peptide modulates lymphocyte migration and natural killer cell activity by a
cyclic adenosine monophosphate (
cAMP)-dependent mechanism , whereas
substance P induces mediator release by a
cAMP - independent mechanism . -
pubmed
Somatostatin antagonizes the effects of both
vasoactive intestinal peptide and
substance P by a mechanism that appears to involve inhibitory
guanine nucleotide binding proteins . -
pubmed
We describe here a limiting dilution analysis to determine cytotoxic T lymphocyte precursor ( CTLp ) frequencies against individual
HLA - A or -B
antigens . -
pubmed
This LDA is a practicable tool for a systematic analysis of CTLp response against selected individual
HLA - A or -B
antigens and can be used for the selection of
HLA mismatched donors for transplantation patients . -
pubmed
( + /-)- Cyanopindolol increased the evoked
3H overflow ; this effect was prevented by preexposure to the previously characterized
serotonin receptor antagonist
metitepin . -
pubmed
The present results show that the presynaptic
serotonin autoreceptor is blocked by
cyanopindolol in a stereoselective way . -
pubmed
This drug is 20 times more potent than
metitepin as an antagonist at the presynaptic
serotonin autoreceptor , and , in contrast to the latter , it does not act as an antagonist at the presynaptic
alpha 2 - adrenoceptor on the serotoninergic neurone . -
pubmed
A possibility of increasing the survival of cells by the pre- and
post - irradiation treatment with a
mesoporphyrin - IX derivative was demonstrated . -
pubmed
A single exon encodes the carboxyl - terminal 26
amino acids of the
ssd chain and the 3 ' untranslated region of its mRNA , ending with a poly(A)-addition site . -
pubmed
Normal serum and
CSF chloride concentrations were restored . -
pubmed
Calculations made on the basis of rate of
CSF production , CSF
chloride concentration , and duration of
anorexia provided supportive evidence for an active transport system for
chloride from plasma to
CSF . -
pubmed
In contrast , hepatic
cholesteryl ester levels , rates of hepatic
LDL transport , and concentrations of plasma
LDL -
cholesterol were not affected by
bile acid feeding in this species . -
pubmed
Ursodeoxycholic acid , in contrast , does not affect these processes either directly or indirectly and so causes no change in plasma
LDL levels . -
pubmed
The
carbohydrate moiety of
human platelet glycocalicin : the structures of the major
Asn - linked
sugar chains . -
pubmed
The released
carbohydrate chains were found to be of the complex - type from their interaction with immobilized
lectin columns . -
pubmed
The structures of the two major
oligosaccharide -
alditols separated by ion - exchange chromatography on a Mono Q column were investigated by means of methylation analysis ,
glycosidase digestion , and Smith
periodate degradation , and they were assigned as typical di- and trisialylated complex - type
oligosaccharide - alditols with two and three peripheral chains consisting of
Gal - GlcNAc sequences , respectively . -
pubmed
Results demonstrated that neonatal hearts had a significantly shorter ( p less than .05 )
TIC ( 29.5 + /- 1.7 min ) than adult hearts ( 43.0 + /- 2.9 min ) and exhibited more rapid
lactate accumulation ( 2.5 + /- 0.5 to 22.1 + /- 3.0 , p less than .001 ) and an increase in G-6 - P ( 0.07 + /- 0.03 to 0.34 + /- 0.07 , p less than .01 ) with a nonsignificant decline in myocardial
glycogen ( 23.3 + /- 12.1 to 17.4 + /- 4.0 , p greater than .05 ) . -
pubmed
The lysosomal degradation of
ganglioside GM2 by
hexosaminidase A depends on the presence of the specific activator protein which mediates the interaction between micellar or membrane - bound
ganglioside and
water - soluble hydrolase . -
pubmed
In the absence of enzyme the activator acts in vitro as
glycolipid transfer protein , transporting
glycolipids from donor to acceptor membranes . -
pubmed
Three duodenal juice samples ( at 30 , 60 and 90 mins of hormonal infusion ) were taken in each subject for
CEA , cytology ,
bicarbonate and
trypsin determinations . -
pubmed
Sequential treatment of
gelatinase A with
4 - aminophenylmercuric acetate (
APMA ) and
neutrophil elastase yielded an active
gelatinase with a 4 - fold increase in gelatinolytic activity . -
pubmed
In the presence of
gelatin , these two inactivating cleavage sites were protected , and cleavage at a site within the
hemopexin - like
carboxyl - terminal domain resulted in a truncated yet active
gelatinase . -
pubmed
We assessed the efficacy and safety of the lipid - lowering drugs ,
nicotinic acid ( short acting ) and
lovastatin , the
3 - hydroxy-3 - methylglutaryl coenzyme A reductase inhibitor . -
pubmed
At 16 weeks , when compared with control values ,
nicotinic acid ( & gt ; or = 1.5 g twice a day ) significantly reduced the total
cholesterol ( from 312 + /- 18 [ + /- SEM ] mg / dL to 229 + /- 19 mg / dL ; P = 0.03 ) and the
low - density lipoprotein cholesterol ( from 218 + /- 15 mg / dL to 142 + /- 13 mg / dL ; P = 0.03 ) and significantly increased the
high - density lipoprotein cholesterol ( from 44 + /- 3 mg / dL to 58 + /- 5 mg / dL ; P = 0.03 ) . -
pubmed
At 16 weeks ,
lovastatin therapy ( 40 mg / d ) significantly reduced the total
cholesterol ( from 285 + /- 13 mg / dL to 233 + /- 10 mg / dL ; P = 0.005 ) and the
low - density lipoprotein cholesterol ( from 201 + /- 11 mg / dL to 147 + /- 7 mg / dL ; P = 0.001 ) . -
pubmed
There were no significant changes in the
triglyceride and
high - density lipoprotein cholesterol levels . -
pubmed
Whole blood samples were collected in
EDTA and assayed using a commercially available radioimmunoassay kit that employs a
monoclonal antibody specific for Cy . -
pubmed
Can tests for
IgA ,
IgG , or
IgM antibodies to
cardiolipin or
phosphatidylserine substitute for lupus anticoagulant assays in screening for antiphospholipid antibodies ? -
pubmed
We evaluated ELISA tests for
IgG ,
IgM , and
IgA isotopes of antibodies binding
cardiolipin (
CL ) and
phosphatidylserine (
PS ) in samples from
LA patients presenting with
recurrent miscarriages . -
pubmed
Neither the use of
PS as the
antigen nor the addition of
IgA assays improved the correlation of ELISA results with the presence of LA . -
pubmed
TNF - alpha - induced adherence to
fibronectin was suppressed from 69 % + /- 5 % of the
phorbol myristate acetate response to 38 % + /- 7 % ( P = 0.0154 ) . -
pubmed
The
androgen receptor (
AR ) gene contains a polymorphic CAG microsatellite that codes for a variable length of
glutamine repeats in the
AR protein . -
pubmed
The deduced
amino acid sequences of the
alpha , beta 2 , beta 3 , beta 4 and gamma isoforms predict molecular weights of 57,600 , 56,500 , 60,900 , 52,500 , and 68,000 , respectively . -
pubmed
Thus both
gonadotropins contribute , directly for
LH and indirectly , through paracrine mecanisms , for
FSH , to
testosterone production . -
pubmed
Concomitantly ,
cocaine prevented the maximal producible
HTR frequency via simultaneous indirect stimulation of the inhibitory presynaptic
5 - HT1A and postsynaptic
alpha 2 receptors . -
pubmed
In the present study , we have investigated the effects of
cocaine and the selective
5 - HT (
sertraline ) ,
norepinephrine (
nisoxetine ) and
dopamine (
GBR 12935 ) uptake inhibitors on the
L - tryptophan - induced
HTR in the presence of a nonselective
monoamine oxidase inhibitor ,
tranylcypromine . -
pubmed
Cocaine potentiated the ability of
L - tryptophan to induce
HTR to a greater extent in protocol 1 , whereas
sertraline induced a greater effect in protocol 2 . -
pubmed
However , in our earlier study
cocaine ( and also
sertraline ) up to 10 mg / kg produced a similar degree of potentiation in both experimental protocols on the
5 - HTP - induced
HTR . -
pubmed
Furthermore , as in the latter study on the
5 - HTP - induced
HTR , in the present investigation
nisoxetine potently attenuated whereas
GBR 12935 did not modulate the induced
HTR . -
pubmed
The results show that the respective serotonergic and noradrenergic effects of
cocaine also operate on the
L - tryptophan - induced
HTR . -
pubmed
The differential effects of
cocaine and
sertraline in experimental protocols 1 and 2 on the
L - tryptophan- versus
5 - HTP - induced
HTRs suggest that
cocaine has additional effects on the conversion of
L - tryptophan to
5 - HT . -
pubmed
The catalytic properties of the
proteases were analyzed by their kinetic constants and by a combination of two - dimensional gelatin -
sodium dodecyl sulfate -
polyacrylamide gel electrophoresis and enzyme overlay membranes . -
pubmed
Both
Pr2 isoforms preferentially cleave at the
carboxyl sides of positively charged
amino acids , preferring
arginine ; the pI 4.4 Pr2 isoform also possessed significant activity against
lysine . -
pubmed
The mean ( + /- SD ) values of serum total
cholesterol ,
low - density - lipoprotein (
LDL )
cholesterol ,
high - density - lipoprotein (
HDL )
cholesterol and
triglycerides in the hypercholesterolemic subjects were 7.2 + /- 1.1 mmol / L , 5.1 + /- 1.1 mmol / L , 1.0 + /- 0.1 mmol / L and 2.4 + /- 0.9 mmol / L , respectively . -
pubmed
Exposure to
MBC resulted in an increased number of spindle
poles associated with chromosomes displaced from the metaphase plate . -
pubmed
Since
platelet - activating factor (
PAF ) is a potent mediator of inflammatory responses , we investigated the potential of
PAF to modulate
IL-6 and
GM - CSF production by EC . -
pubmed
Exposure of these cells to
PAF resulted in a concentration - dependent increase in
IL-6 production , with a maximum at 10(-10 ) M PAF . -
pubmed
This effect was specific for
PAF since it was prevented by preincubation with the
PAF receptor antagonist ,
WEB 2086 . -
pubmed
Pretreatment of EC with the protein synthesis inhibitor
cycloheximide before their exposure to
PAF resulted , after washout of the
cycloheximide , in a markedly augmented production of
IL-6 , suggesting a synergy between augmented IL-6 mRNA accumulation by
PAF and IL-6 mRNA superinduction by
cycloheximide . -
pubmed
GM - CSF production by EC was also stimulated by the combined effects of
PAF and
TNF alpha , but
PAF alone did not affect
GM - CSF production . -
pubmed
Taken together , our data suggest that
PAF can stimulate EC to synthesize
cytokines , including
IL-6 and
GM - CSF , which may contribute to local and , possibly , systemic responses during
inflammation . -
pubmed
0.10 , P & lt ; 0.05 ) , significantly higher levels of
oestradiol post - partum ( 0.7 vs. 0.19 ng / ml , + /-
azaperone ; S.E.D. -
pubmed
gamma - glutamylcysteine synthetase : mRNA stabilization and independent subunit transcription by
4 - hydroxy-2 - nonenal . -
pubmed
gamma - Glutamylcysteine synthetase (
GCS ) , the rate - limiting enzyme in de novo
glutathione (
GSH ) synthesis , is composed of one catalytic ( heavy ) and one regulatory ( light ) subunit . -
pubmed
4HNE increased both the transcription rates and the stability of mRNA for both
GCS subunits , resulting in an increased mRNA content for both subunits . -
pubmed
Emetine , a protein synthesis inhibitor , blocked the increase in
GCS light subunit mRNA but not the increase in
GCS heavy subunit mRNA . -
pubmed
This suggested that although
4HNE increased transcription and stabilization of both
GCS subunit mRNAs , the signaling pathways involved in the induction of the two
GCS subunits differed . -
pubmed
These findings indicate that
IkappaBalpha is imported in the nucleus by a piggy - back mechanism that involves additional protein(s ) containing a
basic NLS and able to interact with
ankyrin repeats of
IkappaBalpha . -
pubmed
The purified
Ftf exhibited maximum activity at pH 6.0 and 37 degrees C , was activated by
Ca2 + , but inhibited by the metal ions
Cu2 + ,
Zn2 + ,
Hg2 + and
Fe3 + . -
pubmed
The
enzyme catalysed the transfer of the
fructosyl moiety of
sucrose to a number of acceptors , including water ,
glucose and
sucrose via a Ping Pong mechanism involving a
fructosyl - enzyme intermediate . -
pubmed
In
diabetes , activation of the
aldose reductase (
AR ) pathway and alterations of
glucose - sensitive signal transduction pathways have been implicated in depletion of intracellular
taurine , an endogenous antioxidant and compatible osmolyte . -
pubmed
Cellular
taurine accumulation occurs by an osmotically induced ,
protein kinase C ( PKC)-regulated
Na (+)- taurine cotransporter (
hTT ) . -
pubmed
In low
AR - expressing cells , 20 mM
glucose decreased
taurine content ,
hTT transporter activity , and mRNA levels , and these effects were unaffected by
AR inhibition ( ARI ) . -
pubmed
In these cells , the inhibitory effects of high
glucose on
hTT appeared to be posttranscriptionally mediated , because 20 mM
glucose decreased hTT mRNA stability without affecting
hTT transcriptional rate . -
pubmed
Inhibition of
PKC overcame the decrease in
hTT activity in high
glucose - exposed cells . -
pubmed
In high
AR - expressing cells , prolonged exposure to 20 mM
glucose resulted in intracellular
taurine depletion , which paralleled
sorbitol accumulation and was prevented by
ARI . -
pubmed
In 5 mM
glucose ,
hTT transcriptional rate was markedly decreased in high
AR - expressing cells , did not decline further in 20 mM
glucose , but was increased by ARI to levels above those observed in low
AR - expressing cells . -
pubmed
Therefore ,
glucose rapidly and specifically decreases
taurine content ,
hTT activity , and mRNA abundance by
AR - unrelated and
AR - related posttranscriptional and transcriptional mechanisms . -
pubmed
Data including age , gender , cause of
end - stage renal disease (
ESRD ) , time on dialysis ,
body mass index (
BMI ) , blood levels of midweek predialysis
albumin ,
prealbumin , and
bicarbonate were collected . -
pubmed
Average values ( + /- 1 SD ) were age 61 + /- 16 years ,
BMI 23.3 + /- 4.6 kg / m2 , dialysis time 12.4 + /- 2.7 h / week ,
HCO3 22.8 + /- 3.5 mmol / L ,
albumin 38.7 + /- 5.3 g / L ,
prealbumin 340 + /- 90 mg / L , Kt / V 1.36 + /- 0.36 , nPCR 1.13 + /- 0.32 g / kg BW / day , and LBM 0.86 + /- 0.21 % of ideal LBM . -
pubmed
Bicarbonate was also negatively correlated with
albumin ,
prealbumin ,
BMI , and LBM . -
pubmed
Chalcone isomerase (
CHI ) catalyzes the intramolecular cyclization of
chalcone synthesized by
chalcone synthase (
CHS ) into
( 2S )- naringenin , an essential compound in the biosynthesis of
anthocyanin pigments , inducers of Rhizobium nodulation genes , and antimicrobial
phytoalexins . -
pubmed
The lower pressure limit of RBF autoregulation was examined before and after infusion of the
NOS inhibitor
N ( G )- monometyl - L - arginine (
L - NMMA ; 500 microg . -
pubmed
As the
GFR was unchanged during
L - NMMA infusion , these observations suggest that postglomerular contraction during
NOS inhibition may be involved in the regulation of
GFR in 70 - week - old SHR . -
pubmed
Immunoblotting experiments showed that
kainate induced more potent expression of
c - Fos protein in nuclear fractions obtained 2h after injection than those obtained 18h later , with similar expression between cytosolic fractions obtained 2 and 18h after administration . -
pubmed
Two - dimensional electrophoresis revealed expression of several proteins immunoreactive to the anti -
c - Fos antibody with different isoelectric points in response to
kainate in nuclear and cytosolic fractions of the hippocampus for 2 - 18h after a single injection . -
pubmed
Immunoprecipitation analysis using the anti -
c - Fos antibody showed phosphorylation of
c - Fos protein on
serine residues in hippocampal nuclear fractions 2h after administration , with additional phosphorylation on
tyrosine residues 18h later . -
pubmed
Prior treatment of the protein synthesis inhibitor
cycloheximide prevented the expression of immunoreactivities to the anti -
c - Fos antibody detected on two - dimensional electrophoresis in hippocampal nuclear fractions obtained 2h after administration . -
pubmed
These results suggest that in vivo
kainate signals may lead to persistent expression of the
transcription factor activator protein-1 that consists of different
Fos family members , as well as of
c - Fos protein phosphorylated on
serine and/or
tyrosine residues , at an early stage after administration . -
pubmed
The heterotrophic and autotrophic alkaliphiles that grew with
thiocyanate as an N source possessed a relatively high level of
cyanase activity which converted
cyanate (
CNO- ) to
ammonia and
CO2 . -
pubmed
On the other hand ,
cyanase activity either was absent or was present at very low levels in the autotrophic
strains grown on
thiocyanate as the sole energy and N source . -
pubmed
keratinocyte growth factor was tested by
[ 3H ] thymidine assay . -
pubmed
The difference in
keratinocyte growth factor receptor and
androgen receptor gene expression after treatment with and without
keratinocyte growth factor and
flutamide were determined by quantitative multiplex PCR and quantitated using densitometry analysis . -
pubmed
Epithelial cells showed a significant time and dose dependent stimulation of cell proliferation with
keratinocyte growth factor and
dihydrotestosterone ( p & lt;0.05 ) . -
pubmed
keratinocyte growth factor quantitative multiplex PCR revealed stimulated
keratinocyte growth factor receptor and
androgen receptor messenger RNA expression . -
pubmed
CONCLUSIONS : These results show that
keratinocyte growth factor up - regulates the
keratinocyte growth factor and
androgen receptors in the absence of
androgen . -
pubmed
Thus , the
androgen signaling pathway may be activated by growth factors such as
keratinocyte growth factor in an
androgen deficient environment . -
pubmed
Treatment with
calcium channel blocker ( CCB)s ,
dihydropyridines and others , is frequently complicated by dependent
oedema in the absence of
sodium retention or
cardiac failure , a bothersome side effect of unclear aetiology . -
pubmed
Differential signalling of both wild - type and
Thr(343)Arg
dopamine D ( 2short ) receptor by partial agonists in a G - protein - dependent manner . -
pubmed
Most of the partial agonists , but not
bromocriptine , displayed an enhanced response at the
mutant D ( 2short ) receptor , suggesting that the mutation brings the receptor in a more active state . -
pubmed
This enhancement was apparent both at the level of
G - protein activation (
[ 35S ] GTPgammaS binding ) and at the effector (
Ca ( 2 + ) response ) and occurred with different
G ( alpha )- proteins . -
pubmed
Lisuride and in particular
bromocriptine produced a more potent response in the co - presence of a
G ( alphao ) protein instead of a
chimeric G ( alphaq / o )- or a promiscuous
G ( alpha15 )- protein . -
pubmed
S (+)- propylnorapomorphine showed a similar partial response irrespective of the combined
G ( alpha )- protein . -
pubmed
Bromerguride and
( + ) - UH 232 induced weak ( 16 to 21 % versus
dopamine ) intrinsic activity in the co - presence of a
G ( alphaq / o )- protein in contrast to their silent properties with a G(alpha15)- or a
G ( alphao ) Cys ( 351 ) Ile - protein . -
pubmed
Chemical modification of mitochondria with the
arginine - specific reagents
phenylglyoxal (
PGO ) and
2,3 - butanedione (
BAD ) decreases the
Ca ( 2 + ) sensitivity of the permeability transition pore (
PTP ) and stabilizes it in the closed conformation ( Eriksson , O. , Fontaine , E. , and Bernardi , P. ( 1998 ) J. Biol . -
pubmed
Unexpectedly , modification of mitochondria with the
arginine - specific reagent
p - hydroxyphenylglyoxal (
OH - PGO ) resulted instead in
PTP opening . -
pubmed
Sequential modification with
OH - PGO and
PGO ( or
BAD ) revealed that the effects on the
PTP depended on the order of the additions . -
pubmed
PTP opening was observed when
OH - PGO preceded , and
PTP closing was observed when
OH - PGO followed , the addition of
PGO ( or
BAD ) . -
pubmed
The differential effects of
OH - PGO and
PGO on the
PTP open probability ( i ) were not modified by the conformation - specific ligands of the
adenine nucleotide translocase bongkrekate and
atractylate ; and ( ii ) were also observed in de - energized mitochondria , indicating that the effect is exerted directly on the
PTP . -
pubmed
OH - PGO dramatically sensitized
PTP opening , which was triggered by depolarization even in the presence of
EGTA . -
pubmed
These data show that
arginine modification modulates the
PTP conformation in a ligand - selective fashion and suggest that the effects of
OH - PGO ,
PGO , and
BAD are mediated by the same
arginine residues . -
pubmed
The results indicate that both
OH - PGO and
PGO react with
arginine at a stoichiometry of 2:1 and form stable adducts that may be feasible to identify the
PTP at the molecular level . -
pubmed
The minimal requirements for
COP I and
COP II vesicle formation from liposomes are the
small GTPases Arf1 and
Sar1 ,
GTP and the coat complexes themselves . -
pubmed
Coat - nucleating factors including specific
GTP -
GDP exchange factors ,
SNAREs and other cargo molecules are likely to determine the site of vesicle formation . -
pubmed
For two - step addition , previously embryos were 2 min pre - equilibrated in 0.5 ml of ( 1:1 )
PBS plus 20 % FCS : vitrification solution without
sucrose . -
pubmed
In experiment 2 , we attempted to substitute the use of
PBS by
HEPES - buffered Ham 's F-10 ( h - CM ) in all cryoprotective solutions or media . -
pubmed
When h - CM was used , a significant reduction in the in vitro embryo development was observed when the
HEPES - buffered groups were compared with one - step ( 20EG-20DMSO+S ) group in s -
PBS ( 35 - 45 % versus 73 % ) . -
pubmed
AIMS : To evaluate the suitability of
chrome azurol S (
CAS ) agar plate assay as a quantitative methodology for siderophore production . -
pubmed
Blood was extracted from the heart to examine the level of
LDL -
cholesterol . -
pubmed
RESULTS : The level of
LDL -
cholesterol in the high fat diet group was 26.5 + /- 7.49 mmol / L , significantly higher than that in the normal diet group ( 0.71 + /- 0.65 mmol / L , P & lt ; 0.05 ) . -
pubmed
OBJECTIVES : To provide context in oncology for the significance of the benefits and cost of combined
androgen blockade (
CAB ) in the treatment of advanced
prostate cancer . -
pubmed
The survival benefit with
anastrozole or
exemestane in advanced
breast cancer , or with the addition of
trastuzumab to standard chemotherapy in metastatic
breast cancer that overexpresses
human epidermal growth factor receptor 2 (
HER2 ) , is approximately 4 to 5 months . -
pubmed
The calculated cost per month of survival benefit with
bicalutamide in
CAB for
prostate cancer is 437 US dollars to 1107 US dollars . -
pubmed
At steady state , it shows alterations which are not detected by the measurement of the
oxygen saturation in the longitudinal
sinus or that of
CBF . -
pubmed
These
SRCs interact directly with liganded
TR and function as adapter
molecules to recruit other coactivators such as
p300 /
CBP . -
pubmed
A combination of chromatographic mobility , immunoassays specific for
digoxin and
dihydrodigoxin , and deglycosylation using
5 - sulfosalicylic acid were used to identify the
DLIF and Dh - DLIF components . -
pubmed
RESULTS : With
cholesterol as precursor , the cells produced
DLIF ( 7.5 mCi / mmol ) with a labeling efficiency of 10 % , whereas with
acetate the cells produced
DLIF ( 72.2 mCi / mmol ) with a labeling efficiency of 0.08 % of the total
DLIF produced . -
pubmed
Mevastatin ( 5 micromol ) , an inhibitor of the enzyme
hydroxymethylglutaryl - CoA reductase and thus of
cholesterol biosynthesis , gave an 85 % decrease in the production of
( 14 ) C - DLIF and
progesterone , but only a modest 15 % decrease in
( 14 ) C - Dh - DLIF production . -
pubmed
Thioredoxin is associated with proliferation ,
p53 expression and negative
estrogen and
progesterone receptor status in
breast carcinoma . -
pubmed
Positive nuclear immunostaining was associated with negative
estrogen and
progesterone receptor status . -
pubmed
Another is
Tim11p , which is required for oligomeric assembly of
F1Fo - ATP synthase , which generates
ATP through oxidative phosphorylation . -
pubmed
Ceramide - induced apoptosis : role of
catalase and
hepatocyte growth factor . -
pubmed
The aim of this study was to elucidate cellular mechanisms involved in
ceramide - induced apoptosis and its attenuation by
hepatocyte growth factor (
HGF ) . -
pubmed
Ceramide - treated cells showed increased
caspase-3 activation and an increase in mitochondrial membrane permeability transition ( MPT ) . -
pubmed
Furthermore ,
ceramide treatment significantly decreased
catalase enzymatic activity and protein expression . -
pubmed
HGF pretreatment ( 20 ng / ml ) significantly inhibited
ceramide - induced apoptosis and reduced the accumulation of ROS , the activation of
caspase-3 , and the increase in
MPT and prevented the reduction in
catalase activity and expression . -
pubmed
Together , the data suggest that
ceramide induces apoptosis in
RPE cells by increasing ROS production ,
MPT , and
caspase-3 activation . -
pubmed
The
ceramide effect is potentiated by
H2O2 and associated with a reduction in
catalase activity , suggesting that
catalase plays a central role in regulating this apoptotic response . -
pubmed
Examples are :
genistein and
EGCG , which effect signaling through the
IGF-1 /
AKT pathway and
resveratrol and
limonen , which do so through the
RAS pathway . -
pubmed
Phenylacetate activates
p53 and
p21 through inhibition of
methyltransferase and farnesylation of the
RAS protein . -
pubmed
Losartan increased blood levels of BK-(1 - 9 ) and hydroxylated
BK-(1 - 9 ) by approximately 2 - fold and reduced the BK-(1 - 7)/BK-(1 - 9 ) ratio by 55 % . -
pubmed
Both
losartan and
eprosartan increased plasma levels of
Ang I ,
Ang II , and
Ang -( 2 - 8 ) , and
eprosartan increased
Ang -( 3 - 8 ) levels . -
pubmed
Ang -( 1 - 7 ) and
Ang -( 1 - 9 ) levels were unchanged . -
pubmed
The reductions in BK-(1 - 7)/BK-(1 - 9 ) ,
Ang II /
Ang I , and
Ang -( 1 - 7 )/ Ang I ratios suggest that the increased
bradykinin levels were the result of reduced metabolism by
ACE and
neutral endopeptidase . -
pubmed
Increased
bradykinin levels may represent a class effect of
AT1 receptor blockers that contributes to their therapeutic actions and may also contribute to the
angioedema that may accompany this therapy . -
pubmed
Mycobacteria produce
beta - lactamases and are intrinsically resistant to
beta - lactam antibiotics . -
pubmed
In addition to the
beta - lactamases , cell envelope permeability and variations in certain peptidoglycan biosynthetic enzymes are believed to contribute to
beta - lactam resistance in these organisms . -
pubmed
Immunofluorescence and ultrathin - section
electron microscopy reveals that in
Odf2(-/- ) cells , distal / subdistal appendages disappear from mother centrioles , making it difficult to distinguish mother from daughter centrioles . -
pubmed
Biochemistry variables were also considered : fasting blood
sugar , total
cholesterol ,
HDL - C ,
LDL - C ,
triglycerides ,
fibrinogen ,
C reactive protein and
homocysteine . -
pubmed
The overall objective was to compare the efficacy of
GnRH ,
porcine LH (
pLH ) and
estradiol cypionate (
ECP ) , in a modified Ovsynch / fixed - time AI ( FTAI ) protocol that included a controlled internal drug [
progesterone ] release ( CIDR ) device . -
pubmed
The first and second hormonal treatments ( with an intervening
PGF treatment on Day -3 ) were
GnRH /
GnRH ( 100 microg ) ,
ECP /
ECP ( 0.5 mg ) ,
pLH /
pLH ( 12.5 mg ) or
GnRH /
ECP , respectively ; pregnancy rates were 58.7 , 66.1 , 45.9 and 48.3 % , respectively (
ECP / ECP>both
pLH /
pLH and
GnRH /
ECP ; P<or=0.05 ) . -
pubmed
The cellular
ATP level at 3 h after the start of
SI was increased compared with that at 2 h , and was almost the same as that before the start of
SI . -
pubmed
The cellular
ATP level at 3 h
SI was significantly reduced by either the inhibition of
nitric oxide synthase (
NOS ) or scavenging of
NO . -
pubmed
Either the inhibition of
NOS or the scavenging of
NO during
SI for 3 h also resulted in a significant decrease in the survival rate of myocytes . -
pubmed
The fluorescent intensity of
DAF - FM was significantly increased at 3 h
SI as compared with that at 2 h
SI , and the increase in
DAF fluorescence during
SI was almost completely suppressed by treatment with
vinyl - L - NIO (
L - VNIO ) , a potent specific inhibitor of
nNOS . -
pubmed
The
inositol 1,4,5 - trisphosphate receptor (
IP ( 3 ) R ) , which forms a homotetrameric
Ca2 + release channel in the endoplasmic reticulum , is one of the key molecules responsible for this regulation . -
pubmed
To promote the
Ca2 + -channel gating and release from the endoplasmic reticulum , IP(3 ) binds to the
amino - terminal region of
IP ( 3 ) R. -
pubmed
OBJECTIVE : To investigate the importance of the phosphorylation of
Ser16 and
Thr17 sites of
phospholamban (
PLN ) on intracellular
Ca2 + (
Cai2 + ) handling and contractile recovery of the
stunned myocardium . -
pubmed
In
PLN - WT , a transient increase in
Thr17 phosphorylation at early reperfusion preceded a recovery of
Ca2 + transient amplitude , virtually completed by the end of reperfusion . -
pubmed
In contrast , in
PLN - DM , there was a poor recovery of
Cai2 + transient amplitude and LVDP was significantly lower ( 28.3+/-6.7 % , n=11 , 30 min reperfusion ) than in
PLN - WT hearts . -
pubmed
Although myofilament
Ca2 + responsiveness and
troponin I (
TnI ) degradation did not differ between groups , the episodes of mechanical alternans , typical of
Cai2 + overload , were significantly prolonged in
PLN - DM vs.
PLN - WT hearts . -
pubmed
CONCLUSIONS :
PLN phosphorylation appears to be crucial for the mechanical and
Cai2 + recovery during stunning and protective against the mechanical abnormalities typical of
Cai2 + overload . -
pubmed
The importance of
PLN phosphorylation would primarily reside in the
Thr17 residue , which is phosphorylated during the critical early phase of reperfusion . -
pubmed
Our results emphasize that , although ablation of
PLN phosphorylation does not affect basal contractility , it does alter
Ca2 + handling and mechanical performance under stress situations . -
pubmed
MEASURES OF OUTCOME : At baseline , and after six and twelve months of supplementation , body composition , bone mineral density , serum
25 OH vitamin D , intact
parathyroid hormone (
iPTH ) ,
osteocalcin ,
decarboxylated osteocalcin , urinary
aminoterminal telopeptide of type I collagen (
NTX ) ,
deoxypyridoline cross links ( Dpd ) and
equol were measured . -
pubmed
The group receiving the special supplement had a significant increase in serum
25 OH vitamin D and a significant decrease in serum
iPTH and decarboxylated
osteocalcin . -
pubmed
Polyurethane foam unit ( PFU ) systems were collected from 11 lakes and three rivers in the Yunnan Plateau , China and , the PFU extrusion liquids were analyzed for
organochlorine pesticides ( OCPs ) by gas chromatography with electron capture detection (
GC -
ECD ) . -
pubmed
At the end of reperfusion , histopathological changes of intestine were observed , and
malondialdehyde (
MDA ) levels ,
superoxide dismutase (
SOD ) ,
catalase (
CAT ) , and
glutathione peroxidase (
GSH - Px ) activities in intestinal tissues were also detected . -
pubmed
RESULTS : The H group showed significantly lower
MDA levels and higher activities of
SOD ,
CAT , and
GSH - Px in the intestinal tissue compared with the IR group . -
pubmed
G quadruplex - based FRET probes with the
thrombin - binding aptamer ( TBA ) sequence designed for the efficient fluorometric detection of the
potassium ion . -
pubmed
The dual - labeled
oligonucleotide derivative , FAT-0 , carrying
6 - carboxyfluorescein (
FAM ) and
6 - carboxytetramethylrhodamine (
TAMRA ) labels at the 5 ' and 3 ' termini of the
thrombin - binding aptamer ( TBA ) sequence 5'-GGT TGG
TGT GGT TGG-3 ' , and its derivatives ,
FAT - n ( n=3 , 5 , and 7 ) with a spacer at the 5'-end of a
TBA sequence of T(m)A ( m=2 , 4 , and 6 ) have been designed and synthesized . -
pubmed
Although larger studies are needed , this case demonstrates that pulsed - dosing with
OSPB enhances
HbF synthesis . -
pubmed
To deliver and retain viable repair cells in a surgically prepared
cartilage lesion , we previously developed an adhesive in situ - gelling cell carrier by suspending cells in a solution of hydroxyethyl cellulose (
HEC ) , which was then mixed with chitosan -
glycerol phosphate to form a chitosan -
GP /
HEC gel . -
pubmed
Monolayer cells survived and proliferated in up to 1 mM of
glyoxal , however only a very narrow range of
glyoxal concentration in chitosan -
GP /
HEC , 0.1 - 0.15 mM , permitted gel formation , cell survival , and cell proliferation . -
pubmed
Chitosan gels containing
HEC required slightly less
glyoxal to solidify . -
pubmed
Rin is activated following stimulation of both
Gsalpha and
Gialpha cascades but does not rely upon
cyclic AMP (
cAMP)- ,
Ca ( 2 +)- , or
Epac - dependent signaling pathways . -
pubmed
RNA interference - mediated
HSP27 silencing suppresses both
PACAP38- and
Rin - mediated neurite outgrowth , while expression of a constitutively active
Rin mutant increases both
HSP27 protein and
phospho -
HSP27 levels , supporting a role for
Rin -
HSP27 signaling in neuronal differentiation . -
pubmed
A novel method of determining portal systemic shunting using biodegradable
99Tcm labelled
albumin microspheres . -
pubmed
The clinical effects of two groups were evaluated after one - week treatment and
uric acid ( UA ) and
C - reactive protein (
CRP ) levels in blood were determined after 1 - month treatment . -
pubmed
This review summarizes evidence that dendritic cells are a part of the mononuclear phagocyte system and are derived from a common precursor , responsive to the same
growth factors ( including
CSF-1 ) , express the same
surface markers ( including
CD11c ) , and have no unique adaptation for
Ag presentation that is not shared by other macrophages . -
pubmed
Moreover , the expression of Bcl-2 mRNA and the protein expressions of
MDR1 ,
LRP , and
P - gp were decreased when compared with those of
DDP groups , respectively . -
pubmed
The effects may be associated with over - expression of
MDR1 ,
LRP ,
P - gp , and
Bcl-2 , which can increase the intracellular
platinum accumulation and induce the cell apoptosis . -
pubmed
We recently showed that
Slc26a9 has both
nCl (-)- HCO ( 3 )(- ) exchanger and
Cl (- ) channel function . -
pubmed
Deletion of the
Slc26a9 STAS domain ( a9 - DeltaSTAS ) virtually eliminated the
Cl (- ) currents with only a modest affect on nCl(-)-
HCO ( 3 )(- ) exchange activity . -
pubmed
These results imply that
Slc26 - STAS domains may all interact with ( R)
CFTR but that the physiological outcome is specific to differing
Slc26 proteins , allowing for dynamic and acute fine tuning of
ion transport for various epithelia . -
pubmed
Although
methyl bromide (
MB ) has been phased out in many agricultural industries , it is still the preferred treatment in the perennial nursery industry and is used under Critical Use Exemptions and Quarantine / Preshipment provisions of the Montreal Protocol . -
pubmed
RESULTS : Reduced rates ( 100 - 260 kg ha(-1 ) ) of MB applied in combination with
chloropicrin (
Pic ) and sealed with a low - permeability plastic film provided weed and nematode control similar to the industry standard rate of 392 kg ha(-1 )
MB : Pic ( 98:2 ) sealed with high - density
polyethylene ( HDPE ) film . -
pubmed
While the water extract of viscous material showed a higher
ACE inhibitory activity than the respective
ethanol extract . -
pubmed
INTERVENTION(S ):
Cetrorelix is used as a
GnRH - antagonist in
ART treatments . -
pubmed
To assess the effect of delayed ovulation on embryonic development ,
cetrorelix was applied concomitantly with follicle stimulation by
pregnant mare serum gonadotropin . -
pubmed
The origin of
NO signaling can be traceable back to the origin of life with the large scale of parallel evolution of
NO synthases (
NOSs ) . -
pubmed
While in early preconditioning
NO is produced by the endothelial and neuronal isoform of
NO synthase , in delayed preconditioning
NO is synthesized by the inducible or ' immunological ' isoform of
NO synthase . -
pubmed
Furthermore , in early preconditioning ,
NO acts through the canonical
cGMP pathway , possibly through
protein kinase G and opening of mitochondrial K(
ATP ) channels . -
pubmed
In late preconditioning , the protection is mediated by
peroxynitrite formed by the reaction of
NO with
superoxide derived from the enzyme
NADPH oxidase . -
pubmed
The mechanisms by which
peroxynitrite exerts its protective effect may include improvement of
post - ischaemic cerebrovascular function , leading to enhancement of blood flow to the ischaemic territory , and expression of prosurvival genes resulting in cytoprotection . -
pubmed
The levels of
parathyroid hormone ,
calcitonin ,
osteocalcin ,
procollagen carboxy - terminal propeptide and
bone alkaline phosphatase were significantly lower in the
Al - treated group than in the control group from days 90 , 30 , 60 , 60 and 90 , respectively . -
pubmed
Argiotoxin in the closed
AMPA receptor channel : experimental and modeling study . -
pubmed
Binding of
argiotoxin in the closed state of
Ca ( 2 +)-permeable
AMPA receptor channels was studied using electrophysiological and molecular modeling approaches . -
pubmed
Experimental study unambiguously revealed that
argiotoxin is trapped in the closed
AMPA receptor channels after agonist dissociation . -
pubmed
The unique sea urchin - type morphology ,
bright and sharply visible emission bands of
lanthanide , and large aspect ratio of
ZnO crystalline nanotips make this novel composite an excellent signal receiver , waveguide , and emitter . -
pubmed
In addition , patients on the
etravirine arm had fewer
AIDS - defining conditions , hospitalizations , and lower mortality compared with the
OBR control arm . -
pubmed
Hydrogen sulfide inhibits the translational expression of
hypoxia - inducible factor-1alpha . -
pubmed
BACKGROUND AND PURPOSE : The accumulation of
hypoxia - inducible factor-1alpha (
HIF-1alpha ) is under the influence of
hydrogen sulfide (
H ( 2 ) S ) , which regulates
hypoxia responses . -
pubmed
The regulation of
HIF-1alpha accumulation by
H ( 2 ) S has been shown , but the mechanisms for this effect are largely elusive and controversial . -
pubmed
This study aimed at addressing the controversial mechanisms for and the functional importance of the interaction of
H ( 2 ) S and
HIF-1alpha protein . -
pubmed
The mechanisms for
H ( 2 ) S - regulated
HIF-1alpha protein levels were determined using short interfering RNA transfection , co - immunoprecipitation and
7 - methyl - GTP sepharose 4B pull - down assay . -
pubmed
KEY RESULTS : The accumulation of
HIF-1alpha protein under
hypoxia ( 1 %
O ( 2 ) ) or
hypoxia - mimetic conditions was reversed by
sodium hydrosulfide (
NaHS ) . -
pubmed
This effect of
NaHS was not altered after blocking the
ubiquitin - proteasomal pathway for
HIF-1alpha degradation ; however , blockade of protein translation with
cycloheximide abolished the effect of
NaHS on the half - life of
HIF-1alpha protein . -
pubmed
Knockdown of
eukaryotic translation initiation factor 2alpha (
eIF2alpha ) suppressed the effect of
NaHS on
HIF-1alpha protein accumulation under
hypoxia . -
pubmed
NaHS inhibited the expression of
VEGF under
hypoxia . -
pubmed
CONCLUSIONS AND IMPLICATIONS :
H ( 2 ) S suppresses
HIF-1alpha translation by enhancing
eIF2alpha phosphorylation under
hypoxia . -
pubmed
The interaction of
H ( 2 ) S and
HIF-1alpha inhibits the angiogenic activity of vascular endothelial cells under
hypoxia through the down - regulation of
VEGF . -
pubmed
In addition to the provision of correct information and the correction of misinformation , clinicians must be aware of and must address barriers to adherence with
AD therapy , especially parent
anxiety about the safety of topical medications (
corticosteroids and topical
calcineurin inhibitors ) . -
pubmed
Genetic studies , however , have allowed to clarify which genetic variations underlie the adverse response to some antiretroviral drugs ( such as
HLA - B*5701 in the treatment with
abacavir ) or the occurrence of
renal complications as the disease progresses . -
pubmed
Altered
nucleotide - microtubule coupling and increased mechanical output by a
kinesin mutant . -
pubmed
Kinesin motors hydrolyze
ATP to produce force and do work in the cell -- how the motors do this is not fully understood , but is thought to depend on the coupling of
ATP hydrolysis to microtubule binding by the motor . -
pubmed
Mutants show weak -
ADP / tight - microtubule binding , instead of tight -
ADP / weak - microtubule binding like wild type -- they hydrolyze
ATP faster than wild type , move faster in motility assays , and assemble long spindles with greatly elongated
poles , which are also produced by simulations of assembly with tighter microtubule binding and faster sliding . -
pubmed
The plasma sample was pretreated by
protein precipitation with acidified
acetonitrile . -
pubmed
Since these
tumors are dependent upon the
PI3 K pathway , we investigated the potential for
tumor response by the targeting of this pathway with
rapamycin , an
mTOR inhibitor . -
pubmed
FDG dual hybrid PET /
CT imaging demonstrated a dramatic
tumor response in the
rapamycin arm and this was confirmed on necropsy . -
pubmed
The
tumor tissue remaining after treatment with
rapamycin demonstrated increased
pERK1 / 2 or persistent phosphorylated
ribosomal protein S6 (
pS6 ) , indicating potential resistance mechanisms . -
pubmed
EGFR tyrosine kinase inhibitors ( TKIs ) such as
gefitinib induce apoptosis of
cancer cells , but prolonged treatment is often associated with acquired resistance . -
pubmed
Here , we identified a novel
MEK1 / 2 inhibitor ,
CZ0775 , and compared its
cytotoxic effects to those of
AZD6244 (
selumetinib ) in
non - small cell lung cancer (
NSCLC ) cell lines harboring
EGFR mutations . -
pubmed
Our results suggest that combination treatment with
CZ0774 and
EGFR inhibitors is a promising therapeutic approach for the treatment of
EGFR - TKI - resistant lung cancers and its effect is mediated by the inhibition of
ERK and the induction of
BIM . -
pubmed
Comparison of the effects of
albumin 5 % , hydroxyethyl starch 130/0.4 6 % , and
Ringer ' s lactate on
blood loss and coagulation after cardiac surgery . -
pubmed
INTRODUCTION :
Cariprazine is an orally active and potent
D3 and
D2 partial agonist with preferential binding to
D3 receptors in development for the treatment of
schizophrenia and
bipolar mania . -
pubmed
Next , using oocytes subjected to
IVM for 12 - 30 hr , sperm penetration was examined after 4 - 18 hr of in vitro fertilization (
IVF ) . -
pubmed
A significant negative correlation between
IVM duration and the timing when 50 % of oocytes were penetrated by sperm after
IVF start was observed ( P<0.01 ) . -
pubmed
In conclusion , under the present IVM /
IVF conditions , it was estimated that oocytes acquired their highest developmental competence at about 30 hr after
IVM start , and thus , the optimal IVM duration was calculated to be about 21 hr . -
pubmed
Treatment with 50 - 100 nmol / L
ZD 7288 significantly inhibited EMG responses ( 16%-62 % , 80 - 20 mmHg
CRD , P & lt ; 0.05 ) and AWR scores ( 24%-37 % , 40 - 20 mmHg
CRD , P & lt ; 0.05 ; 12%-61 % , 80 - 20 mmHg CRD , P & lt ; 0.05 , respectively ) , and significantly increased
pain thresholds ( 32%-77 % , P & lt ; 0.05 ) . -
pubmed
Renin -
angiotensin -
aldosterone system (
RAAS ) activation occurs in
hyperthyroidism , which could cause peri - tubular
hypoxia ,
tubular damage and the development of
aCKD . -
pubmed
Urinary
vascular endothelial growth factor :
creatinine ratio ( VEGFCR ) is postulated to be a marker of tubular
hypoxia . -
pubmed
Direct evidence of spatially selective
iron mineralization using an immobilized
ferritin protein cage . -
pubmed
Here , we conducted quantitative and kinetic analyses of the mineralization behavior of immobilized ( apo)
ferritin on a polyelectrolyte multilayer ( PEM ) using
quartz crystal microbalance ( QCM ) , scanning electron microscopy ( SEM ) , and X - ray photoelectron spectroscopy ( XPS ) techniques . -
pubmed
We demonstrated that the ( apo)
ferritin immobilized on a substrate synthesizes a
ferrihydrite core within the confines of the protein cage ; similar to a solution dispersed system . -
pubmed
In addition , we applied a
ferritin /
apoferritin blended monolayer to the study of
iron mineralization and revealed that biomineralization in this system is spatially selective . -
pubmed
Pyridine analogues of spirocyclic
sigma1 receptor ligands . -
pubmed
Spirocyclic benzopyrans 2 interact with high affinity and selectivity with
sigma1 receptors . -
pubmed
Bioisosteric replacement of the
benzene ring of the
benzopyran substructure with the electron rich
thiophene ring ( 3 ) led to increased
sigma1 affinity . -
pubmed
The most promising sigma1 ligand within this class of compounds is the
pyranopyridine 15 with a double bond in the
pyran ring revealing a Ki - value of 4.6 nM and a very high selectivity ( & gt;217 - fold ) over the
sigma2 subtype . -
pubmed
CLA and
CLNA have isomer - specific , health - promoting properties , including anticarcinogenic , antiatherogenic , anti - inflammatory , and antidiabetic activity , as well as the ability to reduce body fat . -
pubmed
macrocarpon ( fruits as well as leaves ) resulted in a statistically significant decrease in total
cholesterol ,
LDL -
cholesterol ,
VLDL -
cholesterol , and
triglycerides in the treated groups compared with the untreated
hypercholesterolemic group , regardless of the administrated doses . -
pubmed
A significant increase in
HDL -
cholesterol was observed in the treated groups . -
pubmed
RESULTS : In
JN PVP group , mean of 3.26 cc of
polymethylmethacrylate (
PMMA ) were injected and 4.07 cc in BVF
PVP group ( p<0.001 ) . -
pubmed
No significant difference in improvement of VAS score was observed between
JN and BVF
PVP groups ( p=0.43 ) . -
pubmed
Usage of BVF for
PVP may be an alternative to
JN PVP in selected cases . -
pubmed
alcohol challenge ( final target breath
alcohol concentration = 0.06 g / dl ) and reported on
SR and craving . -
pubmed
At baseline , LMD and
AD reported greater stimulation than HD , which carried over
post -
alcohol administration . -
pubmed
Urinary
albumin to
creatinine (
ACR ) and
beta2 microglobulin to
creatinine ratios (
BCR ) are the surrogate and robust markers of
renal glomerulopathy and
tubulopathy , respectively . -
pubmed
ACR & gt ; 300 mg / g
creatinine and
BCR & gt ; 300 mug / g
creatinine were used to define the
renal glomerular and tubular damages , respectively . -
pubmed
Ethanol - induced
GABAA receptor alpha4 subunit plasticity involves phosphorylation and neuroactive
steroids . -
pubmed
GABAA receptors containing
alpha4 subunits are widely implicated in acute
ethanol sensitivity , and their spatial and temporal regulation prominently contributes to
ethanol - induced neuroplasticity in hippocampus and cortex . -
pubmed
However , it is unknown if
alpha4 - containing GABAA receptors in the thalamus , an area of high
alpha4 expression , display similar regulatory patterns following
ethanol administration , and if so , by which molecular mechanisms . -
pubmed
In the current study , thalamic
GABAA receptor alpha4 subunit levels were increased following a 6 - week- , but not a 2 - week chronic
ethanol diet . -
pubmed
Following acute high - dose
ethanol administration , thalamic
GABAA receptor alpha4 subunit levels were regulated in a temporal fashion , as a decrease was observed at 2h followed by a delayed transient increase . -
pubmed
PKCgamma and
PKCdelta levels paralleled
alpha4 temporal expression patterns following
ethanol exposure . -
pubmed
Initial decreases in
alpha4 subunit expression were associated with reduced
serine phosphorylation . -
pubmed
Delayed increases in expression were not associated with a change in phosphorylation state , but were prevented by inhibiting neuroactive
steroid production with the
5alpha - reductase inhibitor
finasteride . -
pubmed
Overall , these studies indicate that thalamic
GABAA receptor alpha4 subunit expression following acute and chronic
ethanol administration exhibits similar regulatory patterns as other regions and that transient expression patterns following acute exposure in vivo are likely dependent on both subunit phosphorylation state and neuroactive
steroids . -
pubmed
Complete refolding of
beta1 - adrenergic receptor occurs in
n - decyl - beta - D - maltoside ( DM ) micelles from a
urea - denatured state , as shown by regain of its original helical structure , ligand binding and protein fluorescence . -
pubmed
G protein - coupled receptor kinases (
GRKs ) are a family of
serine /
threonine protein kinases that terminate
G protein - coupled receptor (
GPCR ) signaling by phosphorylating the receptor and inducing its internalization . -
pubmed
Clinical and genetic features of Argentinian children with
diabetes - onset before 12months of age : Successful transfer from
insulin to oral
sulfonylurea . -
pubmed
Among children with
diabetes diagnosed between 6 and 12months , 1 patient had an
INS mutation and one patient was homozygous for an
SLC19A2 mutation which confirmed a diagnosis of
thiamine - responsive
megaloblastic anaemia syndrome . -
pubmed
Five of the patients with an
ABCC8 or
KCNJ11 mutation have successfully transferred from
insulin to
glibenclamide whist 1 child demonstrated a partial response to
sulfonylurea treatment . -
pubmed
Moreover ,
L - pGlu -( 1 - benzyl )- l - His - l - Pro - NH2 resulted in a substantial reduction in
CA1 ( Cornus Ammonis 1 ) hippocampal neuronal cell
death , inflammatory
cytokines ,
TNF - alpha ,
IL-6 and oxidative stress in hippocampus . -
pubmed
Mechanistically , phosphorylation of
MEK1 / 2 and
ERK1 / 2 in NPCs was enhanced upon
LXR agonist treatment , while abrogation of
MEK /
ERK phosphorylation by the inhibitors
PD98059 and
U0126 impaired the proliferation of wildtype NPCs in the presence or absence of
LXR agonists . -
pubmed
Collectively , our findings suggest that
LXR agonists
GW3965 and
LXR623 can stimulate the NPC proliferation in
LXR- and
MEK /
ERK - dependent manner . -
pubmed
Endothelial function was also improved , as measured by increased
nitric oxide (
NO ) formation and decreased
endothelin (
ET-1 ) protein expression . -
pubmed
Although the incorporation of
AZH was minimally invasive for
PDZ3 , structural analysis of
NNAA - modified proteins through the methodology presented herein should be performed to ensure structural integrity of the studied target . -
pubmed
A positive lymphocyte transformation test ( LTT ) confirmed the implication of
amlodipine in the reaction and showed positivity to another
calcium channel blocker ,
nifedipine , demonstrating cross reactivity . -
pubmed
CONCLUSION : This is the first reported case of
hypersensitivity to
amlodipine in which the involvement of the drug is confirmed by a positive
TTL . -
pubmed
It has been shown that the optimum condition for the modification of MNPs is the reaction at 40 C with an equimolar amount of
PMIDA for 3.5 h. The modified MNPs were characterized by X - ray diffraction , transmission electron microscopy , Fourier transform infrared spectroscopy , thermogravimetric , and
CHN elemental analyses . -
pubmed
The possibility of using colloidal solutions of
PMIDA - modified MNPs as a
T2 contrast agent for liver studies in vivo ( at a dose of 0.6 mg kg-1 ) was demonstrated for the first time . -
pubmed
To experimentally prove the theoretical prediction ,
iodine - doped graphene materials were prepared by one -
pot hydrothermal and ball - milling approaches to realize different doping - sites . -
pubmed
Eight
PFASs were measured in plasma using isotope dilution ultra - performance liquid chromatography / tandem mass spectrometry ( UPLC -
MS /
MS ) . -
pubmed
Bilirubin and hepatic enzymes
alanine aminotransferase (
ALT ) ,
alkaline phosphatase (
ALP ) , and
gamma - glutamyltransferase (
GGT ) were determined in serum using an immunoassay methodology . -
pubmed
RESULTS : The changes in majority of
PFAS concentrations were positively associated with the changes in activity of
ALT ,
ALP , and
GGT and inversely associated with the changes in circulating
bilirubin after adjustment for gender and the time - updated covariates
LDL- and
HDL - cholesterol , serum
triglycerides ,
BMI , statin use , smoking , fasting
glucose levels and correction for multiple testing . -
pubmed
RESULTS : The
LP and MIX silages had lowest pH ( p<0.001 ) on 7 and 48 days , while MIX and CON silages had greatest
lactate concentrations ( p<0.05 ) on 7 and 48 days , respectively . -
pubmed
Moreover , introducing
LDHA mRNA into cells over - expressing miR-33b attenuated the inhibitory effect of miR-33b on the growth and
glucose metabolism in
NSCLC cells . -
pubmed
Taken together , these results confirm that miR-33b is an anti - oncogenic miRNA , which inhibits
NSCLC cell growth by targeting
LDHA through reprogramming
glucose metabolism . -
pubmed
The first one -
pot synthesis of undoped and
Eu doped beta - NaYF4 nanocrystals and their evaluation as efficient dyes for nanomedicine . -
pubmed
The expression of the
tumor necrosis factor - ( TNF - ) alpha ,
interleukin - ( IL - ) 1beta ,
IL-6 ,
cyclic adenosine monophosphate (
cAMP ) ,
cyclic guanosine monophosphate (
cGMP ) ,
phosphodiesterase 2A (
PDE2A ) , and
nuclear factor - ( NF - ) kappaB /
p65 in spinal dorsal horns was measured by enzyme - linked immunosorbent assay ,
polymerase chain reaction , and western blot on day 7 after surgery . -
pubmed
The determined optimum conditions for cell growth of
strain FA - HZ1 were a temperature of 30 C , pH 5.0 , rotation rate of 200 rpm and 0.1 mM
DBF as a
carbon source . -
pubmed
The biochemical and physiological features as well as usage of different
carbon sources by
FA - HZ1 were studied . -
pubmed
The new
strain was positive for
arginine double hydrolase ,
gelatinase and
citric acid , while it was negative for
urease and
lysine decarboxylase . -
pubmed
FA - HZ1 metabolizes
DBF by both the angular and lateral dioxygenation pathways . -
pubmed
In addition , the
strain FA - HZ1 can also degrade halogenated analogues such as
2 , 8 - dibromo dibenzofuran and
4 -( 4 - bromophenyl ) dibenzofuran . -
pubmed
METHODS : The
gonadotropin - releasing hormone (
GnRH ) stimulation test was used to determine the HPG axis status and categorize subjects into two groups ( HPG+ or HPG- group ) . -
pubmed
In this study , two novel composite membranes containing nanoscale
ZIF-8 and
polyphenylene sulfide (
PPS ) nonwoven fabric were prepared via hydrothermal (
PPS -
ZIF-8 ) and biomimetic mineralization (
PPS -
ZIF-8 - BSA ; BSA ,
bovine serum albumin ) approaches . -
pubmed
As for poorly soluble low toxic ( PSLT ) nanomaterials , the US National Institute for Occupational Safety and Health estimated the occupational exposure limit ( OEL ) for
titanium dioxide (
TiO2 ) based on a particle surface area (
SA ) metric . -
pubmed
Severe arterial
hypertension and
hyperandrogenism in a boy : a rare case of
catecholamine- and
beta - HCG - secreting
pheochromocytoma . -
pubmed
Increased
beta - human chorionic gonadotropin (
beta - HCG ) and pubertal
testosterone values with suppressed values of
luteinizing hormone (
LH ) and
follicle - stimulating hormone (
FSH ) suggested an
androgenization secondary to
HCG hyperproduction . -
pubmed
Postoperative resolution of symptoms of
beta - HCG and
catecholamine excess and normalization of hormonal levels confirmed the diagnosis . -
pubmed
Blood samples were taken to assess
glucose ,
insulin ,
estrogen , and
progesterone levels . -
pubmed
Despite significant increases in blood
glucose and
insulin ( P & lt ; 0.001 ) , both central and peripheral arm PWV remained unchanged across time and phase , indicating that neither acute
hyperglycemia nor menstrual phase had an impact on central or peripheral arm arterial stiffness . -
pubmed
The most frequently prescribed regimens were
fludarabine +
cyclophosphamide + rituximab ( FCR , 29.3 % ) ,
bendamustine + rituximab ( BR , 17.7 % ) , and other
rituximab - containing regimens ( 22.0 % ) in the treatment - naive setting ( n = 604 ) , other non - FCR /
BR rituximab - containing regimens ( 38.7 % ) and non -
rituximab - containing regimens ( 28.5 % ) in the relapsed / refractory setting ( n = 945 ) . -
pubmed
METHODS : In this cross - sectional study , 310 patients with newly diagnosed
cancer who were referred to a university hospital in Turkey over a 6 - month period and 218 age - matched healthy individuals as controls were evaluated in terms of
anemia : complete blood count ( CBC ) ,
ferritin ,
transferrin saturation ( TS% ) , serum
iron (
SI ) ,
cobalamin (
B12 ) , and
folate levels . -
pubmed
Folate and
B12 deficiencies were seen more frequently in the
cancer group than in the controls [ 6.5 % and 0.9 % ( p < 0.001 ) ; 39.3 % and 18.9 % ( p < 0.05 ) , respectively ] . -
pubmed
Thus , re - expression of
NAA80 , which leads to re - establishment of
actin 's N - terminal
acetyl group , rescued the Golgi fragmentation , whereas a catalytic dead
NAA80 mutant could neither restore
actin Nt - acetylation nor Golgi structure . -
pubmed
After
quercetin treatment , cellular senescence and apoptosis were evaluated by TUNEL staining ,
X - Gal staining and indirect
peroxidase technique for immunocytochemical analysis of related proteins such as
Bax ,
Bcl-2 ,
caspase-3 ,
Hsp27 ,
Lamin B1 ,
p16 ,
cyclin B1 . -
pubmed
Cell viability was assessed by WTS-1 assay while apoptotic studies were carried out by
DAPI ,
acridine orange ( AO)/
ethidium bromide ( EB ) ,
annexin V +
propidium iodide (
PI ) staining using fluorescence microscopy and flow cytometry . -
pubmed
7 - Methoxyheptaphylline also caused S - phase cell cycle arrest with concomitant concentration - dependent decline in the expression levels of
cyclin A , E and D1 . -
pubmed
It was also seen that increasing doses of
7 - Methoxyheptaphylline led to a dose - dependent decline in the expression levels of
wnt-13a and
beta - catenin . -
pubmed
The implementation of trans - fucosylation patterns investigated in this paper via well - designed
fucosidase mutants and proper reaction conditions may lead to development of an excellent platform , serving both fundamental studies and industrial - scale processes , for valuable
carbohydrates synthesis . -
pubmed
Expression of growth factors (
insulin - like growth factor ( IGF ) 1 ,
IGF2 ,
epidermal growth factor and
transforming growth factor - alpha ) and their receptors was evaluated , with similar expression patterns seen for embryos in sealed culture ( 5 %
O2 , embryo density of 1:2 ) compared with the control group ( embryos cultured in microdrops and placed in a 37 C , 5 %
CO2 water - jacketed incubator ; P>0.05 ) . -
pubmed
In general , intranasal
steroid (
INS ) and oral
steroid for short time help improve the recovery of the olfactory function in case of
olfactory dysfunction after
virus infection . -
pubmed
After the third dose of
durvalumab , his serum
CK level was elevated , and soon thereafter
myalgia of the proximal muscles and
blepharoptosis were observed . -
pubmed
In aortic rings , CmV caused a direct vasoconstrictor effect mediated by
alpha-1 adrenergic receptors and counteracted by
nitric oxide . -
pubmed
While a role for
hypoxia and
hypoxia inducible factor 1alpha has been demonstrated , the contribution of lipid microenvironment and more specifically
oxysterols is yet to be explored . -
pubmed
Chromatin immunoprecipitation analysis confirms the implication of
LXR in the regulation of
GLUT1 and
HK2 CONCLUSIONS AND IMPLICATIONS : While our work supports a role of
oxysterols and
LXR in the modulation of macrophage metabolism in
atheroma plaques , it also highlights some
LXR - independent effects of plaques samples . -
pubmed
Stable microtubules ( as defined by resistance to
Ca2 + ,
drug or cold temperature induced disassembly ) form in abundance during
tubulin assembly in brain crude extracts . -
pubmed
All activity binds to
cation exchangers and to
Ca ( 2 +)-calmodulin affinity columns . -
pubmed
Microtubule stabilization by
MBP and
STOP220 is abolished in the presence of
Ca(2+)-
calmodulin , and inhibition curves are similar for both proteins. -
pubmed
These data suggest that
calcium ions , both those present in the extracellular medium and those bound to the plasma membrane , are required for
isoproterenol - stimulation of
adenylate cyclase . -
pubmed
In addition , it appears that neither the presence of extracellular
calcium ions nor full activation of
adenylate cyclase are required for desensitization . -
pubmed
The development of
ACTH - induced
hypertension was prevented by both
nisoldipine , a
calcium channel blocker , and
minoxidil , a vascular smooth muscle relaxant . -
pubmed
Nisoldipine administration was also found to reverse established
ACTH hypertension . -
pubmed
Expression of the
nifA gene is induced in the symbiotic state and site - directed mutagenesis experiments indicate that
nifA expression is essential for symbiotic
nitrogen fixation . -
pubmed
Non - deleterious inhibition of
endogenous peroxidase activity ( EPA ) by
cyclopropanone hydrate : a definitive approach . -
pubmed
Endogenous
peroxidase activity ( EPA ) poses a serious problem in
immunoperoxidase localization of antigens unable to withstand deleterious effects of
aldehyde fixatives ,
alcohols , and various oxidative reagents . -
pubmed
However , the precise mechanism of the action of these compounds on
EPA generating
proteins is not understood . -
pubmed
Cyclopropanone hydrate is a compound whose inhibitory action on the
heme moiety of
horseradish peroxidase is well understood . -
pubmed
In addition , any gross deleteriousness of
cyclopropanone hydrate towards
immunoperoxidase immunolocalization of three of the most delicate
lymphocyte surface antigens was investigated . -
pubmed
Plasma
ACTH followed the same trend although it reached significance after
ether stress only . -
pubmed
Levels of
corticosteroid binding globulin were also increased , whereas daily circadian rhythm of serum
CORT was blunted . -
pubmed
No significant modifications in plasma
insulin , plasma
glucose and
transglutaminase activity in red cells was induced by
glibenclamide perfusion . -
pubmed
Nevertheless ,
glibenclamide induced a significant decrease ( p less than 0.005 ) in
transglutaminase activity after 20 min of perfusion ( 629.83 + /- 53.08 and 521.18 + /- 43.92 , mean + /-
SE , at 0 and 20 min ) . -
pubmed
No correlation was observed between
glucose or
insulin plasma levels and
transglutaminase activity . -
pubmed
Participation of previously described
carcinogen - binding proteins such as the
Ah receptor ,
h2 - 5S protein ,
4 - 5S receptor for
3 - methylcholanthrene and the
Z - protein fraction was discounted on the grounds of competition binding studies and gel - permeation chromatography . -
pubmed
Aflatoxin B1 co - eluted with the
glutathione S - transferases during gel - permeation and separation of the various
isozymes by cation - exchange chromatography indicated interactions with the
YaYa and
YaYc - forms . -
pubmed
The experiments described here were carried out in order to investigate whether or not the
lectin plays any role in mediating the haemocytic response , by measuring the in vivo cellular response to
galactose- or
sialic acid - rich glycoproteins , either in solution or conjugated to Sepharose beads . -
pubmed
Comparison of the ability of injected solutions to stimulate haemocytic aggregates ( nodules ) showed that
PSM , whether native , desialylated or
pronase - digested to produce small
galactose - containing fragments , stimulated large numbers of nodules , in contrast to either
sialic acid - rich glycoproteins such as
bovine submaxillary mucin (
BSM ) and
fetuin , or the
protein bovine serum albumen (
BSA ) . -
pubmed
Multicellular capsules formed around Sepharose conjugated to
galactose - rich molecules such as
PSM ,
asialo - PSM , asialo - fetuin or asialo - BSM were highly significantly thicker than those formed around control untreated beads , whereas capsules around
BSA ,
fetuin or BSM conjugates were significantly thinner . -
pubmed
We postulate that a
very long chain fatty acyl CoA ( VLCFA CoA ) synthetase , which is different from
long chain fatty acyl CoA synthetase , is required for the effective conversion of
C24:0 acid to
C24:0 CoA. -
pubmed
The kinetic mechanism of the binding and release of
calcium by
troponin and by the complexes
troponin :
tropomyosin ,
troponin :
tropomyosin :
actin , and
troponin (
TN)-
tropomyosin (
TM)-
actin :
myosin subfraction 1 (
SF-1 ) was investigated using
troponin labeled on the
TN - I subunit with the fluorophore
4 -( N - iodoac etoxyethyl - N - methyl )- 7 - nitrobenz-2 - oxa-1,3 - diazole . -
pubmed
Kinetic measurements on
TN or
TN :
TM fitted a single rate process for association or dissociation which is consistent with a model in which the
calcium sites are equivalent and independent and each
calcium induces a change in structure of the complex . -
pubmed
TN :
TM :
actin gave biphasic transients for association and dissociation of
calcium . -
pubmed
The evidence is interpreted in terms of a model in which regulated
actin can exist in two states and the binding of each
calcium and
SF-1 displaces the equilibrium between states . -
pubmed
Saturation of
actin sites with
SF-1 had no effect on the rate constant for association with
TN :
TM in the presence of
calcium . -
pubmed
A specific and sensitive determination of
gamma aminobutyric acid in
CSF and brain tissue by gas chromatography - mass spectrometry . -
pubmed
The accurate measurement of the kinetics of binding of
5 alpha - dihydrotestosterone to the Sertoli cell specific protein ,
androgen binding protein (
ABP ) , has been frustrated by the extremely rapid rate of dissociation of the
ABP -
dihydrotestosterone complex . -
pubmed
We describe a rapid and highly sensitive assay suitable for
ABP quantitation which utilizes
DEAE Bio - Gel and
[ 3H ] dihydrotestosterone . -
pubmed
The assay has been used to accurately measure the rate of dissociation ( 8.25 X 10(-4 ) s-1 , t1/2 14 min ) and the rate of association ( 2.04 X 10(5 ) M s-1 ) of the binding of
[ 3H ] dihydrotestosterone to
rat ABP . -
pubmed
This multipoint assay is extremely rapid such that binding can be measured at equilibrium , it has high precision ( coefficient of variation 3 % ) , and is particularly useful at low
protein concentrations ( 50 ng / ml ) ; furthermore , the assay background of nonspecific
3H - binding is extremely low ( 0.2 % ) . -
pubmed
Since at such low
protein concentrations a 10 point Scatchard analysis can be performed on 1 ml culture medium containing as little as 3 fmol
ABP , the assay is suitable for monitoring changes in ABP secretion resulting from manipulations of cells in culture . -
pubmed
Progesterone ( P ) and
oestradiol-17 beta (
E2 ) were determined from the blood samples by RIA . -
pubmed
No changes in blood pressure were observed after
vasopressin administration , although the ovarian blood flow quickly decreased in parallel with the secretion of P and
E2 . -
pubmed
A similar decapeptide ,
neurokinin B , was also synthesized and conjugated onto
BSA using an alternative
SH - introducing reagent ,
3 -( S - p - methoxybenzyl - thiopropionyl )- thiazolidine-2 - thione , and the above heterobifunctional conjugating reagent . -
pubmed
Effect of
17 - estradiol on pituitary
gonadotropins and luteal function in gilts . -
pubmed
Comparison of the distribution of the proteins in
sucrose versus
metrizamide gradients suggested that
P105 was removed from the free ribonucleoprotein particles before complexing with the ribosomes . -
pubmed
These
apolipoproteins were purified by
DEAE - cellulose chromatography in buffers containing
Triton X-100 . -
pubmed
In addition , the
apolipoproteins showed one band with an apparent molecular weight of 72 000 - 73 000 on
SDS - polyacrylamide gel electrophoresis . -
pubmed
Even though the
apolipoproteins of both species had very similar
amino acid compositions , including a relatively high
glycine content , no immunologic cross - reactivity was observed . -
pubmed
Changes in serum
hydroxyproline ,
hydroxylysine and
collagen - like protein levels , and
collagen peptidase activity were observed in clinical investigations . -
pubmed
Effect of intravenous
digitoxin on inotropy , haemodynamics and
P - Q interval in ischaemic heart disease . -
pubmed
Supernatant fractions ( 100,000 X g ) from isolated nuclei are enriched in complexes containing histones
H3 and
H4 , which are of distinct size ( 5.6S by
sucrose gradient centrifugation , approximate molecular weight of 270,000 by gel filtration ) and negatively charged ( isoelectric at pH 4.4 ) . -
pubmed
These complexes bind to
DEAE - Sephacel and can be separated from
nucleoplasmin . -
pubmed
Bacitracin inhibited degradation in both compartments and , although not eliminating
insulin trafficking , may impair the transfer of
insulin from endosomes to lysosomes . -
pubmed
The chimeric
PrP , designated
MHu2MPrP , differs from
MoPrP by 9
amino acids between residues 96 and 167 . -
pubmed
Glycation of
albumin : reaction with
glucose ,
fructose ,
galactose ,
ribose or
glyceraldehyde measured using four methods . -
pubmed
Albumin was glycated ( nonenzymatically glycosylated ) with
glucose ,
fructose ,
galactose ,
ribose or
glyceraldehyde for 5 , 9 , 15 and 19 days . -
pubmed
The present study determined the effect of genetic
obesity and
phenobarbital (
PB ) treatment on the expression and regulation of the hepatic
cytochrome P450 enzyme (
CYP2C11 ) in Fa/ ? -
pubmed
Treatment with
PB further suppressed hepatic
CYP2C11 protein levels and activities in both fa / fa and Fa/ ? -
pubmed
In animal studies , acute interruption of the activity of renal
alpha-1 adrenoceptors by renal denervation results in an increase in
sodium and water excretion . -
pubmed
Chronic selective blockade of
alpha-1 adrenoceptors by
prazosin in clinical practice has been associated with
sodium retention , however . -
pubmed
, hour 3 , 7.8 + /- .7 versus
6.3 + /- 2.0 mL / min ; P & lt ; or = .05 ) and fractional excretion of
sodium ( e.g. -
pubmed
Although
EAAs can acutely stimulate the neurocircuitry that controls
LH - releasing hormone secretion , the present findings suggest that
EAAs might also exert a long - term stimulatory action by acting further upstream in the photoneuroendocrine pathway . -
pubmed
Two types of
ChAT - immunoreactive synaptic profiles were observed within the ZL and upper SGL using the
electron microscope . -
pubmed
Suramin induces deoligomerization of
human tumor necrosis factor alpha . -
pubmed
Suramin inhibits the biological activity of
human tumor necrosis factor alpha (
TNF ) through a direct action on the ligand rather than on its receptors ( Grazioli , L. , Alzani , R. , Ciomei , M. , Mariani , M. , Restivo , A. , Cozzi , E. , and Marcucci , F. ( 1992 ) Int . -
pubmed
In order to clarify the mechanism whereby
suramin leads to inhibition of
TNF , we investigated the possibility that
suramin might modify the quaternary structure of
TNF which is biologically active as a trimer . -
pubmed
For this purpose we used a new assay ( double streptavidin sandwich assay ) designed for the rapid detection of
oligomer - monomer conversion of
proteins . -
pubmed
Taking advantage of this assay we observed , upon incubation with
suramin , dissociation of
TNF . -
pubmed
Suramin - induced dissociation of
TNF was confirmed by gel filtration chromatography . -
pubmed
We conclude that this
familial AD may originate from the missense mutation 717Val -- >
Ile in the
amyloid precursor protein gene and that the clinical picture is typical of
AD , except for normal - pressure
hydrocephalus and
psychiatric phenomena . -
pubmed
Merbarone is a catalytic inhibitor of
DNA topoisomerase ( topo ) II that does not stabilize DNA -
topo II cleavable complexes . -
pubmed
Although the
cytotoxicity of and resistance to complex - stabilizing
topo II inhibitors , such as
etoposide , is thought to be mediated through stabilization of these complexes , the mechanisms of
cytotoxicity and resistance to catalytic inhibitors are not well known . -
pubmed
These novel cell lines are cross - resistant to complex - stabilizing
topo II inhibitors ,
etoposide ,
teniposide ,
amsacrine , and
doxorubicin , but not to other catalytic inhibitors ,
aclarubicin or
SN-22995 . -
pubmed
Of considerable interest , these cell lines are cross - resistant to
SN-38 , a putative
topo I inhibitor , but cross - resistance to other
topo I inhibitors (
camptothecin and
topotecan ) was lower and not seen in every cell line . -
pubmed
By contrast , there was a low correlation between
merbarone and
etoposide and between
SN-38 and other
topo I inhibitors . -
pubmed
We propose the hypothesis that decreased
topo IIalpha may play a role in the resistance to
merbarone that is different from that to complex - stabilizing drugs . -
pubmed
We also found that
DNA - protein complexes stabilized by
SN-38 might be different from those stabilized by
topo II inhibitors and blocked by
merbarone . -
pubmed
Morphine analgesia elicited from the
PAG is respectively decreased by selective serotonergic and opioid receptor antagonists administered into the RVM , and increased by RVM
neurotensin antagonists . -
pubmed
Since
glutamate and
excitatory amino acid ( EAA ) receptors are also active in the RVM , the present study evaluated whether either competitive (
AP7 ) or non - competitive (
MK-801 )
N - methyl - D - aspartate (
NMDA ) antagonists or a
kainate /
AMPA (
CNQX ) antagonist microinjected into the RVM altered
morphine ( 2.5 micrograms ) analgesia elicited from the
PAG as measured by the tail - flick and jump tests . -
pubmed
A comparison of this messenger site with the
S15 ribosomal binding site was conducted by analysing
hydroxyl radical footprintings of these two sites . -
pubmed
It has several functions : firm and compliant structural sustenance of capillaries , regulation of the ultrafiltration coefficient by means of the capillary surface area control , phagocytosis and removal of macromolecules and immune complexes , hormone secretion and production of bioactive substances (
endothelin ,
prostaglandins ,
renin ,
cytokines , immunomodulatory peptides , components of the extracellular matrix etc . ) -
pubmed
As previously observed , inhibition of AC activity by
diazepam was biphasic , with the first phase being receptor - mediated , whereas the second phase involves a direct action on the
enzyme itself . -
pubmed
An examination of [
3H]
diazepam binding revealed a significant increase in the density of
binding sites in denervated striata , with no change in affinity . -
pubmed
A time - dependent increase in
[ alpha-32P ] GTP labeling of two distinct striatal proteins with apparent molecular masses of 40 and 45 kDa , suggestive of the alpha subunits of
Gi and
Gs , respectively , was observed . -
pubmed
These data indicate that the sensitization of the receptor - mediated inhibitory effect of
diazepam on AC activity in denervated striata may involve up - regulation of
BZ receptors as well as enhanced functional coupling of these receptors to inhibitory
G proteins . -
pubmed
The infant was additionally investigated by whole - body scintigraphy with
99mTc - labeled red blood cells to determine the possibility of coexistence of other
visceral hemangiomas and by MR , in which the
tumor manifested as multiple well - circumscribed space - occupying nodules of high signal intensity on
T2 - weighted images with evidence of fast flow . -
pubmed
When vesicles composed of an equimolar mixture of
sphingomyelin ,
phosphatidylcholine ,
phosphatidylethanolamine , and
cholesterol are treated with
phospholipase C , phospholipid hydrolysis occurs without major changes in vesicle architecture . -
pubmed
In the same way , addition of
sphingomyelinase leads only to
sphingomyelin cleavage . -
pubmed
The contribution of both enzymes is unequal , the main role of
sphingomyelinase being the production of relatively large amounts of
ceramide that will facilitate the lamellar - to - nonlamellar transition in the formation of the fusion pore , whereas
phospholipase C provides mainly a localized , asymmetric , high concentration of
diacylglycerol that constitutes the trigger for the fusion process . -
pubmed
GTP cyclohydrolase I controls the de novo biosynthetic pathway of
tetrahydrobiopterin and
folic acid . -
pubmed
Nonphotochemical
chlorophyll fluorescence quenching increased with leaf temperature in a manner consistent with inhibited
CER and
Rubisco activation . -
pubmed
Heat stress led to decreased
3 - phosphoglyceric acid content and increased
ribulose-1 , 5 - bisphosphate content , which is indicative of inhibited metabolite flow through
Rubisco . -
pubmed
Conceptual translation of the
SRK cDNA revealed that the catalytic domain of
SRK was highly homologous to that of
p70S6 K , and that the treatment of
wortmannin or
rapamycin strongly inhibited the phosphorylation and the activation of
SRK , as in
p70S6K. -
pubmed
The
CD4 lymphocyte counts did not differ significantly between
TB and
DMAC patients ( 26 x 10(6)/L in
TB patients vs 5 x 10(6)/L in DMAC patients ) . -
pubmed
The chiral labeled
cystine (
( 2 ) H ( beta2 )- cystine ) was prepared by selective
hydrogen exchange catalyzed by
cystathionine gamma - synthase . -
pubmed
Treatment of either cell line with the
methyltransferase inhibitor
adenosine dialdehyde dramatically shifts
hnRNP A2 localization from the nuclear to the cytoplasmic compartment , as shown both by immunoblotting and by immunocytochemistry . -
pubmed
In vitro radiolabeling with
[ ( 3 ) H ] AdoMet of
GST - tagged
hnRNP A2 RGG mutants , using recombinant
protein arginine methyltransferase (
PRMT1 ) , shows ( i ) that
hnRNP A2 is a substrate for
PRMT1 and ( ii ) that methylated residues are found only in the RGG domain . -
pubmed
The data suggest that
hnRNP A2 may contain a novel nuclear localization sequence , regulated by
arginine methylation , that lies in the R191 - G253 region and may function independently of the
M9 transportin-1 - binding region in
hnRNP A2 . -
pubmed
This study was aimed at determining whether disruption of the Golgi - vesicular traffic by
brefeldin A would change the
acetylcholine release from these cells and affect proteins involved in transmitter release like the 15 kDa proteolipid , common to
V - ATPase and mediatophore . -
pubmed
Physiological characterization showed that
rep1 mutations greatly reduced a subset of
phytochrome A - regulated responses , including the inhibition of hypocotyl elongation , cotyledon expansion , modulation of gravitropic growth of hypocotyl , and induction of the
CAB ( encoding
chlorophyll a / b binding protein ) gene , without affecting the accumulation of
anthocyanin , far - red - preconditioned blocking of greening , induction of germination , and induction of
CHS ( encoding
chalcone synthase ) and
FNR ( encoding
ferredoxin - NADP (+ ) oxidoreductase ) genes . -
pubmed
Molecular cloning and characterization of the
REP1 gene revealed that it encodes a light - inducible , putative transcription factor containing the
basic helix - loop - helix motif . -
pubmed
Citrate accumulation and secretion are physiological functions of the prostate gland that are regulated by
testosterone and
prolactin . -
pubmed
The metabolic pathway for
citrate production in the prostate involves the activity of mitochondrial
aspartate aminotransferase (
mAAT ) . -
pubmed
To measure
acetylcholine release , the cells on the coverslips were pre - incubated with
[ 3H ] choline ( 0.1 micromol / L ) , superfused with modified Krebs - Henseleit buffer at 25 degrees C and electrically stimulated twice for 2 min (
S1 , S2 ; 3 Hz , 0.5 ms , 90 - 100 mA ) . -
pubmed
Anti - phosphotyrosine fluorescence intensity in muscle cells of arterioles maintained at 100 mmHg was reduced by the
tyrosine kinase inhibitor
tyrphostin A47 ( 30 microM ) and increased by the
tyrosine phosphatase inhibitor
pervanadate ( 100 microM ) . -
pubmed
A mutagenesis of the chromosomal
glnD gene was carried out by insertion of an interposon carrying the
kanamycin resistance gene of Tn5 . -
pubmed
Amidated and
glycine - extended
gastrins were detected with specific antisera and their structures determined by mass spectrometry . -
pubmed
These data suggest that two enzymes process the
amino terminus of
gastrin-17 . -
pubmed
Pyrrolidone carboxylic acid peptidase removes the
amino terminal
pyrrolidone carboxylic acid (
pyroGlu ) , forming
gastrin-16 . -
pubmed
Glycine - extended forms of
gastrin-16 and
gastrin-17 comprises 45 % of the total
gastrin immunoreactivity . -
pubmed
Increases of 24 - h
SBP and 24 - h
PP after salt loading correlated directly with changes in
ADMA ( partial R2 = 0.16 for 24 - h
SBP and 0.17 for 24 - h
PP , P & lt ; 0.05 for both ) and inversely with changes in EDD ( partial
R2 = 0.13 , P = 0.09 for 24 h SBP and partial
R2 = 0.15 , P = 0.07 for 24 - h
PP ) , after adjustment for age and
cholesterol . -
pubmed
The key genes
nahAc and
xylE of the
naphthalene catabolism of fluorescent Pseudomonas spp . -
pubmed
Catalytic properties of
D - amino acid oxidase in
cephalosporin C bioconversion : a comparison between
proteins from different sources . -
pubmed
Lacking an efficient process to produce
7 - aminocephalosporanic acid from
cephalosporin C in a single step ,
d - amino acid oxidase (
DAAO ) is of foremost importance in the industrial , two - step process used for this purpose . -
pubmed
gracilis
enzyme when the bioconversion is carried out at saturating
oxygen concentration ) . -
pubmed
We also provide evidence that relief of the negative effect of the AML1 sites is important for
Ca ( 2 + ) activation of the GM -
CSF promoter / enhancer and that
AML1 overexpression increases this
Ca ( 2 + ) activation . -
pubmed
Purified
ATM was phosphorylated on
serine 1981 and was active towards a variety of known
ATM substrates , including
p53 and the
Bloom Syndrome helicase ,
BLM . -
pubmed
The protein kinase activity of
ATM was selectively inhibited by
wortmannin ,
caffeine and
LY294002 and was stimulated by charged biological polymers , including single - stranded M13 DNA ( ssDNA ) , sheared double - stranded calf thymus DNA ,
heparin sulfate and
poly ADP - ribose (
PAR ) , raising the possibility that charged structures may contribute to regulation of
ATM activity . -
pubmed
However , chemical inhibition of the formation of
poly ADP - ribose in cells had no effect on the activation of
ATM - dependent pathways by ionizing radiation . -
pubmed
Moreover , dephosphorylation of
serine 1981 did not affect the apparent molecular weight of
ATM in irradiated extracts . -
pubmed
Our results suggest that phosphorylation of
serine 1981 alone may not directly regulate the subunit composition of
ATM . -
pubmed
Women carrying
BRCA1 and
BRCA2 mutations face difficult and confusing reproductive decisions that fall into three categories : issues relating to risk - reducing surgeries , issues relating to use of
oral contraceptives / tubal ligation , and issues relating to pregnancy and breastfeeding . -
pubmed
Oral contraceptives potentially protect mutation carriers against
ovarian cancer but increase the risk of early - onset
breast cancer , and evidence for the efficacy of tubal ligation in reducing
ovarian cancer risk in
BRCA mutation carriers is contradictory . -
pubmed
A DFT study of the mechanism of
Ni superoxide dismutase (
NiSOD ): role of the active site
cysteine-6 residue in the oxidative half - reaction . -
pubmed
From the
Ni ( III )- O2H species ,
H2O2 formation occurs through a proton donation by
His1 via
Tyr9 , which relative to the resting position of the
enzyme is exothermic by 4.9 kcal / mol . -
pubmed
On the other hand , however , the geometry of the base pair between
5 - methylaminomethyluridine and
G ( III ) in a crystal of the ribosomal 30S subunit was quite different from the predicted one [ Murphy
IV et al . -
pubmed
Salicylic acid (
SA ) participates in the local and systemic response , but
SAR does not require long - distance translocation of
SA . -
pubmed
SAR can be mimicked by foliar
JA application and is abrogated in mutants impaired in
jasmonate synthesis or response . -
pubmed
One of these shRNAs strongly protected cells from
tert - BHP and
H ( 2 ) O ( 2 ) by specifically reducing the expression of
retinol saturase , an enzyme that converts
all - trans - retinol (
vitamin A ) to
all - trans-13,14 - dihydroretinol . -
pubmed
Under basal conditions ,
perilipin restricts the access of cytosolic
lipases to lipid droplets and thus promotes
triacylglycerol storage . -
pubmed
Quantitative analyses by LC /
MS in multiple reaction monitoring mode revealed that
resveratrol diglucoside and
cis - resveratrol acetyhexoside accumulated up to 2.79 and 10.38 microg / g , respectively , while
trans - resveratrol acetylhexoside was barely detectable . -
pubmed
To begin to understand the mechanisms for the development of acute
insulin resistance ,
serine phosphorylation of
insulin receptor substrate ( IRS )- 1 and
c - Jun N - terminal kinase phosphorylation / activation was investigated . -
pubmed
This study was performed to determine whether
dexamethasone (
DEX ) had an effect on
calcium - activated potassium channels ( K(Ca ) channels ) and Occludin protein in blood - brain tumor barrier ( BTB ) . -
pubmed
We compare the adsorption behavior of wild and surface - modified
ferritin on a homopolymer blend of
poly ( desaminotyrosyl tyrosine dodecyl ester carbonate ) (
PDTD ) and
poly ( epsilon - caprolactone ) (
PCL ) . -
pubmed
Wild
ferritin was alkylated by zero length cross - linking to surface
carboxylate groups activated by water soluble
carbodiimide (
EDC ) . -
pubmed
Transmission electron microscopy shows a clear selectivity of alkylated
ferritin adsorption onto the
PCL phase while wild
ferritin predominantly adsorbs onto the
PDTD phase . -
pubmed
However , we demonstrate that
PKR and
eIF2alpha phosphorylation at
serine 51 are not downstream of
p53 in response to
doxorubicin treatment suggesting that
PKR is not a universal target of
p53 as proposed . -
pubmed
However , several recent developments have seriously threatened the prominence of this drug class : ( i ) the adoption of an early invasive strategy , frequently leading to percutaneous coronary intervention ( PCI ) where the dosing and monitoring of LMWH is unfamiliar to most operators , ( ii ) the results of the SYNERGY trial , which not only failed to establish the superiority of
enoxaparin over unfractionated heparin with respect to efficacy , but also demonstrated more
bleeding with LMWH , and ( iii ) the results of the REPLACE-2 and ACUITY trials , which have demonstrated the advantages of an
ACS and PCI treatment strategy based on direct
thrombin inhibition with
bivalirudin . -
pubmed
A key concept that is presented is that the
ACT is a more accurate measure of
LMWH - induced anticoagulation than of
UFH - induced anticoagulation . -
pubmed
These guidelines may facilitate continued use of
LMWH , which has the potential to reduce cost ( less expensive than
bivalirudin ) , diminish the need for intravenous medication ( can be administered subcutaneously in the noninvasive setting with minimal to no monitoring ) , and provide an ideal anticoagulant during PCI ( easy to monitor with the
ACT , at least partially reversible with protamine in the event of
coronary perforation , effective
antithrombin with no platelet activation , thereby potentially reducing the need for routine adjunctive IIb / IIIa inhibition ) . -
pubmed
TBT shifts the Th1 / Th2 balance to Th2 through reduced
IL-12 production by dendritic cells and augmentation of
IL-10 production by B cells , indicating that
TBT contributes to the increase in the number of
allergic diseases . -
pubmed
Given that
azanucleosides affect
human leukocyte antigen expression and lymphocyte reactivity , we conducted a retrospective study to define the impact of pre - HCT
azanucleoside therapy on
post - HCT donor chimerism . -
pubmed
DUSP1 /
MKP-1 is implicated in cellular protection against a variety of genotoxic insults including
hydrogen peroxide , ionizing radiation , and
cisplatin , but its role in the interplay between different
MAPK pathways in determining cell
death and survival is not fully understood . -
pubmed
The one -
pot difunctionalization of
dibromopyridines was also realized using the reagent stoichiometrically . -
pubmed
The
lectin haemagglutinating activity was optimum between pH 8.0 and 9.0 and inhibited after incubation with
D - galactose and its derivatives , especially
alpha - methyl - D - galactopyranoside . -
pubmed
We noted no apparent interaction of
1,25 ( OH )( 2 ) D ( 3 ) with
epidermal growth factor ,
insulin - like growth factor ,
cholera toxin , or
transforming growth factor - beta , but we did find synergistic inhibitory effects of
1,25 ( OH )( 2 ) D ( 3 ) with
suramin and
retinoic acid . -
pubmed
Using computer simulations to determine the limitations of dynamic
clamp stimuli applied at the
soma in mimicking distributed conductance sources . -
pubmed
The data from
3 -( 4,5 - dimethylthiazol-2 - yl )- 2,5 - diphenyltetrazolium bromide test ,
Hoechst33342 staining , terminal deoxynucleotidyl transferase
dUTP - mediated nicked end labeling assay , and
Bax /
Bcl-2 ratio analysis indicated that
salidroside pretreatment attenuated
hypoxia - induced apoptotic cell
death of primary cultured cortical neurons in a dose - dependent manner . -
pubmed
Moreover , preliminary exploration of the possible mechanisms suggested that the protective effects of
salidroside , shown in our experimental setting , might probably be mediated by enhancing the expression of
hypoxia - inducible factor-1alpha , alleviating the increase of intracellular reactive
oxygen species levels , and inhibiting over - expression of
nuclear factor - kappa B protein . -
pubmed
Homology modeling of mosquito
cytochrome P450 enzymes involved in
pyrethroid metabolism : insights into differences in substrate selectivity . -
pubmed
The
CYP6AA3 model has a large active site allowing it to accommodate multiple conformations of
pyrethroids . -
pubmed
The predicted
CYP6P7 active site is more constrained and less accessible to binding of
pyrethroids . -
pubmed
The absence of
CYP6P8 activity toward
pyrethroids appears to be due to its small substrate access channel and the presence of R114 and R216 that may prevent access of
pyrethroids to the
enzyme heme center . -
pubmed
Information obtained using homology models has the potential to enhance the understanding of
pyrethroid metabolism and detoxification mediated by
P450 enzymes . -
pubmed
We have identified and synthesized a series of
imidazole containing dimerization inhibitors of
inducible nitric oxide synthase (
iNOS ) . -
pubmed
The
SLC45A3 -
ELK4 fusion , but not wild - type ,
ELK4 plays important roles in regulating cell growth in both
androgen - dependent and -independent
prostate cancer cells . -
pubmed
We extend our previous work on the conformation switching of polyzwitterionic brushes grafted onto
ECP to the case of ABC random polyampholyte brushes : here , a statistical near - neutral copolymer of
methyl methacrylate (
MMA ) ,
methacryloxyethyltrimethylammonium chloride (
METAC ) , and
3 - sulfopropyl methacrylate potassium salt (
SPMA ) . -
pubmed
The behavior can be contrasted with that of zwitterionic brushes , which show a switch between collapsed with
ECP oxidized and expanded with
ECP reduced , independent of salt concentration over the same range ( 10(-3)-2 M
NaCl ) as that studied here , and that of zwitterionic - hydrophobic block copolymers where the switch is suppressed at low salt concentration . -
pubmed
What have we learnt about
high - density lipoprotein cholesterol measurements during 32 years ? -
pubmed
BACKGROUND :
High - density lipoprotein cholesterol (
HDL - C ) is important in risk assessment for
cardiovascular disease or
metabolic syndrome ; however , different direct
HDL - C assays may lead to erroneous risk estimates and potentially misclassify people . -
pubmed
The formation of a polar pocket involving
Asn202 in the lumen of the tetrameric
TM bundle is crucial for the assembly and solvation of the domain . -
pubmed
Amino acid variation within the
P2 domain of the major
capsid protein ,
VP1 , was followed longitudinally within the GII.4 lineage . -
pubmed
The configuration at
C6 in the
7 - carba analogs does not influence the rate of acid - catalyzed hydrolysis but has a minor influence on the rate of spontaneous hydrolysis of the
2,4 - dinitrophenyl galactosides , confirming earlier studies on the role played by the
hydroxymethyl group conformation on
glycoside reactivity . -
pubmed
Results show that 31 % and 0.5 % of the total aboveground
carbon storage in the LS and
PB , respectively was located in high forest damage risk ( HR ) zones . -
pubmed
RESULTS :
Titanium (
Ti ) or
polymethylmethacrylate (
PMMA ) particles ( 0.1 mg / mL ) did not significantly change the viability of BMMs , but twice increased the differentiation of BMMs into mature osteoclasts , and markedly increased
TNF - alpha production . -
pubmed
The
TNF - alpha level in the
PMMA group was significantly higher than in the
Ti group ( 96 h ) . -
pubmed
Furthermore , VIVIT reduced
TNF - alpha production much more efficiently in the
PMMA group than in the
Ti group ( 96 h ) . -
pubmed
It is concluded that
MDP can significantly enhance the NOD2 mRNA and protein expression in sensitized DC , promote the expression of
HLA - DR , synergistic
costimulatory molecules and
adhesion molecules of DC , at the same time ,
MDP can increase secretion of
inflammatory factors IL-12p40 . -
pubmed
Hemoglobin A1c ( HbA1c ) is widely used in the general population for
diabetes diagnosis and monitoring as it highly correlates with blood
glucose levels and outcomes . -
pubmed
Autophagy induced by
EEF2 K silencing promotes cell survival and does not potentiate the anticancer efficacy of the
AKT inhibitor
MK-2206 . -
pubmed
N - terminal sequencing of the
trypsin - activated
Cry7Aa fragments revealed that processing occurs at
Glu(47 ) for the 70 - kDa form or
Ile(88 ) for the 65 - kDa form . -
pubmed
Here we report engineered variants of the photoconvertible
Eos fluorescent protein that fluoresce and photoconvert normally in heavily fixed ( 0.5 - 1 %
OsO4 ) , plastic resin - embedded samples , enabling correlative super - resolution fluorescence imaging and high - quality electron microscopy . -
pubmed
Discovery of
DS-5272 as a promising candidate : A potent and orally active
p53 -
MDM2 interaction inhibitor . -
pubmed
We have published
p53 -
MDM2 interaction inhibitors possessing a novel
dihydroimidazothiazole scaffold . -
pubmed
APCcy and
APCox induced a robust T cell
BrdU incorporation . -
pubmed
Acetylcholine enhances keratocyte proliferation through
muscarinic receptor activation . -
pubmed
Activation of the
MAPK /
Erk signaling pathway and its involvement in
ACh - enhanced proliferation was determined by western blot analysis ,
MTS , and
BrdU ELISA . -
pubmed
Western blot analysis revealed that
ACh stimulation of keratocytes upregulated the expression of
PCNA and
Ki-67 , and
Ki-67 immunocytochemistry showed that
ACh - treated cells were in an active phase of the cell cycle . -
pubmed
ACh activated
MAPK signaling , and this step was crucial for the
ACh - enhanced proliferation , as inhibition of the
MAPK pathway resulted in
ACh having no proliferative effect . -
pubmed
Accumulating evidence has suggested that
DES with
cobalt chromium stent platform , modified biodegradable polymer coatings , and
rapamycin derivative drugs are associated with improved clinical outcomes . -
pubmed
Short - term exposure to PM2.5 and
dLAN upregulated neuroinflammatory
cytokines and altered
CA1 hippocampal structural changes , as well as provoked
depressive - like responses (
anhedonia ) . -
pubmed
The Vivartana treated
CTX induced group shows increase in the
hemoglobin level compared with the
CTX induced group . -
pubmed
Similarly the lowered bone marrow cellularity and number of
alpha - esterase positive cells in
CTX induced group were restored in the Vivartana treatment . -
pubmed
Treatment with vivartana also exhibits hepatoprotective activity by regulating the
SGOT and
SGPT levels in
CTX induced group . -
pubmed
The two
isoflavones were effective in enhancing the activity of NSAID activated gene (
NAG-1 ) at 2.5 pg / mL ( 1.5 - 1.8 fold increase ) and inhibiting
iNOS and
NF - kappaB activity with IC50 values in the range of 6 - 13 microg / mL. -
pubmed
As observed by transmission electron microscopy (
TEM ) , MPE ,
OSA and
POM also inhibited
arachidonic acid - induced
tau fibrillization in a concentration - dependent manner . -
pubmed
Meanwhile , the changes of
LC3 - II and
P62 levels and formation of autophagosome suggested that
resveratrol in combination with
cisplatin triggered autophagy . -
pubmed
Significant RAS activates the
renin -
angiotensin -
aldosterone system and is associated with three major clinical
syndromes :
ischemic nephropathy ,
hypertension , and destabilizing
cardiac syndromes . -
pubmed
The longitudinal and transverse axes of 4 experimental groups (
Tic1 , Tic2 , Zrc1 , and Zrc2 ) were analyzed ( vertical / horizontal adjustment ) using a scanning
electron microscope at x1000 magnification . -
pubmed
Acetazolamide enhances the release of urinary exosomal
aquaporin-1 . -
pubmed
A previous study has demonstrated that
acetazolamide ( AZ ) , a diuretic that inhibits
carbonic anhydrases , alters the expression level of
AQP1 in cultured cells . -
pubmed
Treatment with
furosemide did not change , whereas
NaHCO3 and
NH4Cl decreased the exosomal release of
AQP1 . -
pubmed
Anti -
Ro60 were purified by elution from native
Ro60 - coated ELISA plates and subjected to combined de novo
amino acid sequencing and database matching . -
pubmed
Specific
IgV amino acid substitutions stratified anti -
Ro60 from anti -
Ro60 /
La responses , providing a molecular fingerprint of
Ro60 / La determinant spreading and suggesting that different forms of
Ro60 antigen drive these responses . -
pubmed
After 8 - week treatment , the increase in roughness caused by 16 %
CP was significantly higher ( p & lt ; 0.05 ) than the one caused by 40 %
H2O2 . -
pubmed
Bleaching with
CP 16 % resulted in higher roughness than bleaching with
H2O2 , while 40 %
H2O2 caused the higher microhardness increase . -
pubmed
Effects of endurance exercise and
doxorubicin on
skeletal muscle myogenic regulatory factor expression . -
pubmed
Endurance exercise enhances
MRF expression and thereby may mitigate
DOX - induced MRF disruptions . -
pubmed
This study examined the effects of endurance training and
DOX treatment on
myogenic regulatory factor (
MRF ) expression . -
pubmed
Results : Significant
Myf5 drug and activity effects were observed in the soleus with EXER+
DOX expressing higher
Myf5 than SED+
DOX . -
pubmed
No main effects or interactions were observed in the
EDL , but in the diaphragm , a significant activity effect was observed for
Myf5 with EXER+
DOX expressing higher levels than SED+
DOX . -
pubmed
D - Dimer Levels and Effect of
Rivaroxaban on Those Levels and Outcomes in Patients With
Acute Coronary Syndrome ( An ATLAS
ACS - TIMI 46 Trial Substudy ) . -
pubmed
It was hypothesized that elevated baseline D - dimer would be associated with poor clinical outcomes in
ACS , and that
Factor Xa inhibition with
Rivaroxaban would reduce D - dimer acutely and chronically . -
pubmed
In conclusion , elevated baseline D - dimer was associated with an increased risk of the composite outcome within 6 months of the
ACS event and administration of the
Factor Xa inhibitor
rivaroxaban was associated with lower D - dimer levels compared with placebo after the first dose , at day 30 and day 180 . -
pubmed
Sunitinib is an orally administered
tyrosine kinase inhibitor . -
pubmed
The aim of the present study was to examine the light stability of
sunitinib and confirm the effects of light exposure on
sunitinib measurements by LC -
MS /
MS . -
pubmed
Ribosome - inactivating proteins (
RIPs ) are enzymes with
N - glycosylase activity that remove
adenine bases from the ribosomal RNA . -
pubmed
It is noteworthy that this is the first study presenting the complete
amino acid sequence of a
RIP from a Gypsophila species . -
pubmed
The full
amino acid sequence of
gypsophilin - S was assembled by MALDI -
TOF -
MS -
MS and PCR . -
pubmed
The
isophthalate - induced proteins include a
phenylacetate : CoA ligase , a
UbiD - like decarboxylase , a
UbiX - like
flavin prenyltransferase , and a hypothetical protein . -
pubmed
Subsequently ,
benzoyl - CoA is metabolized by the
enzymes of the anaerobic
benzoate degradation pathway that were identified in the proteomic analysis . -
pubmed
In vitro
enzyme assays with cell - free extracts of
isophthalate - grown cells indicated that
isophthalate is activated to
isophthalyl - CoA by an
ATP - dependent isophthalate : CoA ligase (
IPCL ) , and subsequently decarboxylated to
benzoyl - CoA by a
UbiD family isophthalyl - CoA decarboxylase (
IPCD ) that requires a
prenylated flavin mononucleotide (
prFMN ) cofactor supplied by
UbiX to effect decarboxylation . -
pubmed
Homologs of the
IPCD encoding genes are found in several other bacteria , such as aromatic compound - degrading denitrifiers , marine
sulfate - reducers , and methanogenic communities in a
terephthalate - degrading reactor . -
pubmed
Both
PQQ-42 and PQQ-44 were found to degrade a wide range of
N - acylhomoserine lactone (
AHL ) QS signals , possibly due to the presence of an
aac gene which encodes an
AHL amidohydrolase . -
pubmed
The Correlation between
Malondialdehyde and
Nerve Growth Factor Serum Level with
Diabetic Peripheral Neuropathy Score . -
pubmed
AIM : This study was conducted to identify
malondialdehyde (
MDA ) serum level ,
nerve growth factor (
NGF ) serum level ,
diabetic peripheral neuropathy score and the correlation between
MDA and
NGF serum level with
diabetic peripheral neuropathy score . -
pubmed
There were significant correlations between the
MDA serum level and the
diabetic peripheral neuropathy score ( r = 0.364 , p = 0.048 ) , and between the
NGF serum level with the
diabetic peripheral neuropathy score ( r = -0.59 , p = 0.001 ) . -
pubmed
Low
NGF serum level plays a bigger role than high
MDA serum level in
diabetic peripheral neuropathy . -
pubmed
BaB2
O3 F2 : A
Barium Fluorooxoborate with a Unique
2 [ B2 O3 F]- Layer and Short Cutoff Edge . -
pubmed
BaB2 O3 F2 exhibits a unique
oxyfluoride layer 2 [ B2
O3 F]- and a deep - ultraviolet cutoff edge below 180 nm . -
pubmed
Octreotide - LAR in later - stage
autosomal dominant polycystic kidney disease (
ALADIN 2 ): A randomized , double - blind , placebo - controlled , multicenter trial . -
pubmed
This review summarizes novel imaging in the management of
prostate cancer including multiparametric MRI , PET -
CT scans with different radiotracers including
11C - acetate ,
11C - choline ,
18F - choline ,
18F sodium fluoride ,
prostate - specific membrane antigen , and
anti-1 - amino-3 -[ 18F ] fluorocyclobutane-1 - carboxylic acid (
fluciclovine ) . -
pubmed
The PCSK9i that are currently licensed for clinical use ,
evolocumab and
alirocumab , have shown high potency in lowering LDL -
cholesterol , which can exceed 60 % , and other favourable effects on lipid profiles , including a very marked reduction of non - HDL
cholesterol and
apolipoproteinB. -
pubmed
CONCLUSION : Nonstudy oral
PrEP use in the first phase 2 study of
tenofovir reduced -
glycerin 1 % gel was high at a single site in
SF where community
PrEP availability and use was expanding . -
pubmed
The VPP can be used to evaluate automated
insulin and
glucagon delivery algorithms , so - called artificial pancreas ( AP ) algorithms that are currently being used to help people with
T1D better manage their
glucose levels . -
pubmed
Consequently , persisting adaptive changes may occur in the
glucose -
insulin metabolism , including reduced capacity for
insulin secretion and
insulin resistance . -
pubmed
Doxorubicin conjugated AuNP / biopolymer composites facilitate cell cycle regulation and exhibit superior
tumor suppression potential in
KRAS mutant
colorectal cancer . -
pubmed
The degradation of physiological
sAbeta was prevented by a highly selective
insulin - degrading enzyme inhibitor ( Ii1 ) but not by a phagocytosis inhibitor (
polyinosinic acid ) or pinocytosis inhibitor (
cytochalasin B ) in vitro . -
pubmed
Materials and Methods : Here , in our designed synthesis route , the traditional
FeCl3 precursor was replaced with
Fe2O3 nanospheres , and then the
Prussian blue (
PB ) nanoparticles were engineered into hollow - structured
PB ( HPB ) nanospheres through an interface reaction , where the
Fe2O3 colloidal template provides
Fe3 + ions . -
pubmed
Meanwhile ,
HPB nanospheres also demonstrated a high
DOX loading capacity of 440 mg g-1 as a drug carrier , and the release of the drug can be regulated by the heat from
PB shell under the exposure of an NIR laser . -
pubmed
Blood tests were executed to assess the blood
glucose and lipid indicators , including HbA1c , fasting plasma
glucose ( FPG ) ,
triglyceride ( TG ) , total
cholesterol ( TC ) ,
low - density lipoprotein cholesterol (
LDL - C ) ,
high - density lipoprotein cholesterol (
HDL - C ) ,
apolipoprotein B100 (
apoB100 ) ,
prebeta1 - HDL ,
HDL3b ,
apoB100 / AI ,
apolipoprotein AI (
apoAI ) ,
HDL2b and
HDL2a . -
pubmed
Subsequently , four decades later , Ingram and colleagues characterized the A to T substitution in DNA producing the GAG to
GTG codon and replacement of
glutamic acid with
valine in the sixth position of the
betaS - globin chain . -
pubmed
In this mini review , we will critically review previous and ongoing clinical trials of anti -
CD20 monoclonal antibodies in
MS , including
rituximab ,
ocrelizumab ,
ofatumumab , and ublituximab . -
pubmed
Studies on
calcium ion - induced conformation changes in the
actin - tropomyosin -
troponin system by fluorimetry . -
pubmed
Conformational changes in the region containing fluorescence - labeled
sulfhydryl group(s ) of
troponin . -
pubmed
Conformational changes associated with the functional states of the molecule of
troponin were studied using
SH - direct fluorogenic reagents ,
N -( p -( 2 - benzimidazolyl ) phenyl ) maleimide (
BIPM ) and
N -( 1 - anilinonaphthyl-4 ) maleimide (
ANM ) . -
pubmed
On addition of
Ca2 + , the fluorescence characteristics of the two systems ,
ANM -
troponin . -
pubmed
When
BIPM was used in place of
ANM , similar changes were observed : a simple decrease in the intensity when pCa was decreased from 7.4 to 5.5 in the system without
F - actin and a sigmoidal increase in the range from pCa 7 to 6 in the system with
F - actin . -
pubmed
Heavy
meromyosin , when added to the latter complex ( the reconstituted thin filaments ) , made the profile of its
Ca2 + concentration dependence of fluorescence similar to that of the former complex . -
pubmed
The data obtained imply that the
Ca2 + -induced conformational changes of
troponin are markedly modified when detached from
actin , and that
heavy meromyosin weakens the interaction of the
troponin . -
pubmed
A
phosphodiesterase inhibitor ,
diazoxide (
Dx ) , caused insignificant increase in the basal
cAMP , without affecting the force of contraction or CF . -
pubmed
The activity of this
enzyme was manifested mainly in the conditions of continuous cultivation at a rate of 0.1 - -0.2 hr-1 and a final
nitrogen concentration of 0.006 - -0.025 g per litre of the medium . -
pubmed
If the concentration of
nitrogen was increased in these conditions , biosynthesis of
L - glutaminase -
asparaginase I was stimulated while the activity of
L - glutaminase -
asparaginase II was not detected at all . -
pubmed
Under conditions favourable for
phospholipase - induced mitochondrial swelling on account of
phospholipase activation ( low
Mg2 + concentration , and
Ca2 + present in the incubation medium ) the hormone proved to accelerate the swelling of the organellae . -
pubmed
When
phospholipase - induced mechanism of mitochondrial swelling was inhibited ( high
Mg2 + concentration )
thyroxin depressed considerably the swelling of organellae induced by lipid peroxidation due to its antioxidant action . -
pubmed
The extent of the inhibitory effect of
ricin in
polyphenylalanine synthesis by eukaryotic ribosomes is strongly dependent upon the concentration of ribosomes and the
elongation factors EF 1 and
EF2 . -
pubmed
Similarly , the enzymatic binding of
Phe - tRNA to ribosomes is drastically blocked in ricin - treated ribosomes when low
EF 1 concentrations are added to the reaction mixture , but there is no inhibition when
EF 1 is at saturating concentrations . -
pubmed
Furthermore , formation of the complex
EF 2 -
guanosine triphosphate - ribosome , using free ribosomes pretreated with
ricin , is strongly inhibited at limiting concentrations of
EF2 , but is not affected at saturating concentrations of this factor . -
pubmed
However ,
ricin does not inhibit the
EF 2 - dependent translocation of peptidyl - tRNA by polysomes , although the toxin is very active in preventing
amino acid incorporation by polysomes . -
pubmed
Maximal expression of
Ia antigen and release of
interleukin 1 (
IL1 ) were observed during the first week of pregnancy ( implantation ) , when the highest peak of
estradiol was produced . -
pubmed
Although
PGE2 production had no evident relation with
Ia antigen expression and
IL1 activity during the first and second weeks of pregnancy , the increment in
PGE2 values and percentages of NBT+ cells could indicate a different stage of macrophage activation . -
pubmed
Insulin - like growth factor I modulates voltage - dependent
Ca2 + channels in neuronal cells . -
pubmed
The effect of
IGF - I occurred after 1 - 2 h incubation and reversed within 24 h.
Ca2 + channel currents recorded in control cells were mainly of a low - threshold fast inactivating type and showed a mean density of 5.9 + /- 0.3 pA / pF. -
pubmed
Ca2 + channel currents in cells treated with
IGF - I showed an enhanced amount of a high - threshold slowly inactivating
Ca2 + current type sensitive to the
dihydropyridine isradipine and the snail toxin omega - conotoxin . -
pubmed
The effect of
IGF - I was suppressed by coincubation with the
PKC inhibitors
1 -( 5 - isoquinolinylsulfonyl )- 2 - methyl - piperazine (
H-7 ) and
staurosporin which were both without effect on current density in control cells . -
pubmed
The effects of
IGF - I and
phorbol ester were not additive . -
pubmed
Post - aSNA responded with a tendency to increase after the
2 - DG injection . -
pubmed
The increase in adipocyte differentiation in response to
Dex was associated with a decrease in c - fos and actin RNA expression whereas the decrease in c - fos RNA expression in response to
Dex was small ( approximately 40 % ) ; immunocytochemical analysis indicated that induction of
Fos protein occurred only in undifferentiated cells . -
pubmed
Expression of
IGF - I RNA and secretion of
IGF - I and
PGE2 were also decreased in response to
Dex treatment . -
pubmed
Deletion of the
carboxyl - terminal tail domain of either protein indicated that this region was not absolutely essential for co - assembly into the endogenous
vimentin cytoskeleton . -
pubmed
Thus , an intact alpha - helical rod domain was essential for normal IF co - assembly whereas
carboxyl - terminal deletions into this region resulted in dramatic alterations of the existing type III and
IV intermediate filament cytoskeletons in vivo . -
pubmed
The interaction between
EF - Tu and mitochondrial
Phe - tRNA was investigated by using the ability of
EF - Tu to protect the
aminoacyl - tRNA bond from hydrolysis . -
pubmed
coli
EF -
Tus are capable of interacting with mitochondrial
Phe - tRNA . -
pubmed
However , ribosomal A - site binding assays demonstrated that efficient binding of the mitochondrial
Phe - tRNA to the ribosomal A - site was only obtained with the homologous
mitochondrial EF - Tu . -
pubmed
Serum immunoglobulins (
IgG ,
IgA ,
IgM ) were measured as well as total and active
E - RFC and cellular immunity was evaluated using the intradermal testing of
PPD ,
candidin D-7 ,
trichophytin D-5 and
tetanus anatoxin . -
pubmed
Early afterdepolarizations and delayed afterdepolarizations can both be suppressed by low external
Ca2 + concentrations or by
Ca2 + channel blockers . -
pubmed
Comparison of
proline transport and
oxygen uptake by membrane vesicles of the wild - type transductant and the mutant (
ttr-3 ) transductant showed that membrane vesicles of the mutant exhibited temperature - sensitive decrease of
proline transport and increase of
oxygen uptake at the restrictive temperature ( 42 degrees C ) , compatible with depression of growth of the mutant at this temperature . -
pubmed
Significant interaction between low - spin
iron ( III ) site and
pyrroloquinoline quinone in active center of
nitrile hydratase . -
pubmed
Nitrile hydratase , a unique non -
heme iron enzyme , contains low - spin
Fe ( III ) site and
pyrroloquinoline quinone in the active center . -
pubmed
The enzymatic activity of
nitrile hydratase is strongly inhibited by typical
carbonyl reagents such as
phenylhydrazine and
semicarbazide . -
pubmed
Of special interest is the fact that these
carbonyl reagents induced significant alteration of the ESR features of the native
iron ( III ) enzyme at pH 7.2 . -
pubmed
In addition , the
isobutyronitrile ( inhibitor )- bound enzyme was also remarkably affected by
phenylhydrazine and then the transformed
ESR characteristics were different from those of
phenylhydrazine - treated enzyme . -
pubmed
The present results reveal the first evidence for an important interaction between the low - spin
Fe ( III ) site and
pyrroloquinoline quinone which is essential for the activity of
nitrile hydratase . -
pubmed
Molecular biology of
gelsolin , a
calcium - regulated
actin filament severing protein . -
pubmed
In the presence of micromolar
Ca2 + ,
gelsolin severs
actin filaments , causing profound changes in the consistency of
actin polymer networks . -
pubmed
A variant of
gelsolin containing a 25 -
amino acid extension at the NH2 - terminus is present in plasma where it may be involved in the clearance of
actin filaments released during tissue damage . -
pubmed
The NH2 - terminal half of the molecule contains a
Ca2 + -insensitive
actin severing domain while the COOH - terminal half contains a
Ca2 + -sensitive
actin binding domain which does not sever filaments . -
pubmed
These data suggest that the NH2 - terminal severing domain in intact
gelsolin is influenced by the
Ca2 + -regulated COOH - terminal half of the molecule . -
pubmed
These cells are known to respond to
estradiol by increasing
progesterone receptor levels ,
alkaline phosphatase activity , and cell density . -
pubmed
Nuclease S1 was used to recognize and cleave regions made permanently single - stranded due to
osmium recognize and cleave regions made permanently single - stranded due to
osmium modification . -
pubmed
In linearized plasmids treatment with
Os , py produced no
S1 - detectable site - specific modification . -
pubmed
This
H2O2 - dependent
mutagenicity of
DCB was prevented by
horseradish peroxidase . -
pubmed
These results suggest that mechanisms , perhaps
enzymes endogenous to tester
strains TA98 , may play a role in the activation of
DCB . -
pubmed
Elimination of hippocampal
CA3 and
CA4 pyramidal cells with
kainic acid ( KA ) induces a performance deficit on a forced - choice alternation task , which recovers over time . -
pubmed
The three activities appear to be different since
cysteine inhibited
casein digestion ,
aurothiomalate inhibited
histone digestion , and neither inhibited proteoglycan digestion . -
pubmed
A total of 215 of these were tested for
hepatitis B antigen (
HB Ag ) by radioimmunoassay and 32 ( 15 % ) were positive . -
pubmed
There is a relatively small , but constant , amount of
superoxide released from alveolar macrophages at
rest . -
pubmed
This
syndrome is characterized by excess
triglyceride (
TG ) accumulation in the liver and apparent decreased hepatic lipoprotein output . -
pubmed
Phytic acid is undigestible by the monogastric but rumen
phytases are assumed to adequately hydrolyze it . -
pubmed
In above three species the synthesis of longer chain
mycolic acids ( longer than C44 or C46 ) was inhibited more significantly than shorter homologues such as C38 or
C40 . -
pubmed
Furthermore , mass fragmentographic analysis , monitoring fragment ions ( A ) and ( B ) , due to straight chain and branched chain
alkyl units , respectively , demonstrated the inhibition of
mycolic acids was not attributed to the shortening of
alpha - alkyl chain , but to the inhibition of chain elongation of C28 to
C32 straight chain
meromycolic acids . -
pubmed
Furthermore our data demonstrate the presence of
thioesterase activity associated with liver , lung , kidney and brain microsomes capable of hydrolysing
TTPG and thus liberating free
thiol groups . -
pubmed
Role of intracellular
Ca2 + levels in the regulation of
CD11a / CD18 mediated cell adhesion . -
pubmed
The results demonstrate that , in addition to
protein tyrosine kinase (
PTK ) and
protein kinase C (
PKC ) mediated signalling , increase in cytosolic - free
calcium ( [
Ca2+]i ) levels play a major role in the activation of
LFA-1 . -
pubmed
The
calcium ionophore
Ionomycin , which increases [
Ca2 +]i is capable of directly activating
LFA-1 . -
pubmed
Furthermore , activation of
LFA-1 by triggering through
CD2 ,
CD3 or
MHC class II is associated with an increase in [
Ca2 +]i levels , with kinetics that directly correlate with cell adhesiveness . -
pubmed
Moreover , entry of extracellular
Ca2 + via
Ca - channels is involved in both the
CD3- and
MHC class II , as well as part of the
CD2 induced
LFA-1 activation . -
pubmed
Depletion of intracellular
calcium results in unresponsiveness of
LFA-1 to these stimuli , further demonstrating a regulatory role for [
Ca2 +]i in
LFA-1 mediated adhesion . -
pubmed
We also investigated the effects of
superoxide dismutase conjugated to
polyethylene glycol and
catalase , but the number of
vomiting episodes and latency did not change significantly when these agents were intraperitoneally injected 30 min prior to or 20 min after the administration of
cisplatin . -
pubmed
The
CKII target sites have been mapped to multiple
serine and
threonine residues on 4 tryptic fragments within the C - terminal 350
amino acids of
yeast topoisomerase II . -
pubmed
Because both
cytokines and neurohormones may influence the macrophage system , the current study was done to evaluate the effects of
IL-3 and of the pineal hormone
melatonin (
MLT ) on monocyte response to
IL-2 administration . -
pubmed
CONCLUSIONS : These results suggest that
IL-3 alone or in association with
MLT may modulate macrophage functions during
cancer immunotherapy with
IL-2 and decrease the
IL-2 - induced macrophage activation . -
pubmed
In order to determine the role of the C - terminal region of
DSR - S in dextran or
oligosaccharide synthesis , four
DSR - S genes with deletions at the 3 ' end were constructed . -
pubmed
In fact , it seems that the role of the C - terminal domain of
DSR - S may be to facilitate the translation of dextran and
oligosaccharides from the catalytic site . -
pubmed
Three fractions were recovered : UF was
NT non - retained in immobilised
AMP ; F - I was bound
enzyme eluted with
beta - glycerophosphate , and F -
II was bound
NT released with
AMP . -
pubmed
eNT was 80000 - fold purified in F -
II , this fraction showing proteins of 74 , 68 and 51 kDa after immunoblotting . -
pubmed
NT in UF migrated at 6.7S after centrifugation in
sucrose gradients with
Triton X-100 , the peak being split into two of 6.7S and
4.4S in gradients with
Brij 96 . -
pubmed
Ecto -
NT in F - I or F -
II migrated at 5.8S in
Triton X-100- , or 4.4S in
Brij 96 - containing gradients . -
pubmed
A
zinc / protein molar ratio of 2.2 was determined for
eNT in F -
II . -
pubmed
NT activity was decreased in assays made in
imidazole buffer , and was partly restored with 10 microM
Zn2 + or 100 microM
Mn2 + . -
pubmed
In assays with
Tris buffer ,
NT showed a Km for
AMP of 12 microM , and was competitively inhibited by
ATP or
ADP . -
pubmed
A novel mutation at a probable
heme - binding ligand in
neutrophil cytochrome b558 in atypical
X - linked chronic granulomatous disease . -
pubmed
A membrane - bound
cytochrome b558 , a heterodimer consisting of
gp91 - phox and
p22 - phox , is a critical component of the
superoxide (
O2-)-generating reduced
nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase in phagocytes . -
pubmed
We report here an atypical case of
X - linked CGD in which neutrophils showed a complete absence of
O2 - -forming
NADPH oxidase activity , but a small amount ( about 10 % of control ) of both subunits was detected by immunoblot analysis . -
pubmed
Spectrophotometric studies of the neutrophils with a recently developed sensitive method gave no evidence for the
heme spectrum in the
cytochrome b558 , of this
CGD . -
pubmed
Reverse transcription /
polymerase chain reaction and sequence analysis revealed a C to T transition replacing
histidine at
amino acid position 101 (
His101 ) by
tyrosine in
gp91 - phox . -
pubmed
These results provide evidence that
His101 of
gp91 - phox is the one of the
heme - binding ligands of
cytochrome b558 . -
pubmed
The contractions of normal uterine smooth muscles to
oxytocin " 0.1 , 1 and 10 mU " and to
ACh " 0.1 , 1 and 10 uM " were significantly reduced in the presence of
EAC cells . -
pubmed
Metallated species (
Cd ,
Zn and mixed
Cd -
Zn complexes ) were decomposed on - line to produce
apo metallothioneins of which the molecular masses were determined by
MS . -
pubmed
Carbachol and
endothelin-1 increased
GTP - bound
p21 ( ras ) in a
pertussis toxin - sensitive manner [ ratio of
[ 32P ] GTP to ( [
32P]GTP +
[ 32P ] GDP ): control , 30 + /- 1.7 ; 3 min of 1 microM
carbachol , 39 + /- 1.1 ; 3 min of 1 microM
endothelin-1 , 40 + /- 1.2 ] , whereas
histamine ,
bradykinin , and
KCl were without effect . -
pubmed
Transfection of an inhibitor of the
G protein betagamma - subunit [ the
carboxy terminus (
Gly495 -
Leu689 ) of the
beta - adrenoceptor kinase 1 ] failed to inhibit the
carbachol - induced activation of
p21 ( ras ) . -
pubmed
They are
cysteine - rich glycoproteins predominantly made of multiple repeats homologous to the
calcium - binding
epidermal growth factor module , and are translated as precursor proteins cleaved by furine / PACE - like activities . -
pubmed
Ionization of this
glutamic acid side chain causes a pH - dependent decrease in the thermal stability of leishmania
TIM . -
pubmed
The presence of this
glutamate , also in its protonated state , disrupts to some extent the conserved
hydrogen bond network , as seen in all other
TIMs . -
pubmed
METHODS : Venous blood samples were drawn every 2 h over 25 h for measurements of
atrial natriuretic peptide (
ANP ) ,
brain natriuretic peptide (
BNP ) ,
norepinephrine ,
renin activity , and
aldosterone and two urine collections ( 12 h each ) for stable
prostaglandin metabolite measurements . -
pubmed
Plasma
renin activity and
aldosterone did not differ between the groups . -
pubmed
CONCLUSIONS : We conclude that mild
preeclampsia is associated with elevated plasma
norepinephrine levels , lower systemic daytime production of
prostacyclin , and blunting of the normal diurnal variation for a number of indices including plasma levels of
BNP ,
ANP ,
norepinephrine , and
aldosterone , and urinary
prostacyclin metabolites . -
pubmed
Recombinant
epoetin therapy and correction of the chronic
anemia of
renal failure have greatly reduced the number of red cell transfusions and hence the propensity to
iron overload . -
pubmed
Iron overload before the availability of
epoetin constituted a serious problem ; our review of the literature does not decisively conclude that these patients had more serious
bacterial infections or increased mortality when compared with their non -
iron overloaded counterparts , unless chronic transfusion - related
hepatic disease was superimposed . -
pubmed
Optimized
anemia therapy in
ESRD requires individualized and specific application of
epoetin and
iron for each patient , and significant cost savings can result from such a strategy . -
pubmed
Whether
ferritin should be supplanted by another parameter and whether
iron itself poses an increased risk to those patients it has so beneficially served are issues that must be resolved . -
pubmed
Here , we show that cross - linking of the
beta ( 2 ) (
CD18 ) or beta(3 ) (
CD61 ) , but not
beta ( 1 ) ( CD 29 ) integrins in the presence of a low dose of
formyl - Methionyl - Leucyl - Phenylalanine (
fMLP ) enables similar 8 - s periodic RALS oscillations in suspended PMN in response to stimulation with two consecutive doses of chemoattractants . -
pubmed
However , the
A1 receptor agonist
2 - Chloro - N6 - cyclopentyladenosine and the
A3 receptor agonists
N6 - Benzyl - NECA and
1 - deoxy-1 -[ 6 -[[( 3 - iodophenyl ) methyl ] amino ]- 9H - purin-9 - yl ]- N - methyl - be ta - d - ribofuranuronamide expressed only weak inhibitory effects . -
pubmed
On the other hand ,
NECA and
CGS-21680 dose - dependently potentiated the production of
IL-10 . -
pubmed
The differential effect of these drugs on monocyte
IL-12 and
IL-10 production implies that these effects are mediated by
A2a receptor signaling rather than by intracellular toxicity of
ADO analogue 's metabolites . -
pubmed
Moreover ,
CGS-21680 inhibited
IL-12 production independently of endogenous
IL-10 induction , because
anti - IL-10 Abs failed to prevent its effect . -
pubmed
The selective
A2a antagonist
8 -( 3 - Chlorostyryl ) caffeine prevented the inhibitory effect of
CGS-21680 on
IL-12 production . -
pubmed
The
phosphodiesterase inhibitor
Ro 20 - 1724 dose - dependently potentiated the inhibitory effect of
CGS-21680 and , furthermore , Rp - cAMPS , a
protein kinase A inhibitor , reversed the inhibitory effect of
CGS-21680 , implicating a
cAMP /
protein kinase A pathway in its action . -
pubmed
Activated neutrophils release a lot of mediators including toxic
oxygen metabolites ,
elastase and
cytokines which damage brain tissue . -
pubmed
Therefore , we investigated roles of
neutrophil elastase in the development of
cerebral damage using an
elastase inhibitor ,
ONO-5046 . -
pubmed
NR1 subunit pore mutations that block
Ca ( 2 + ) influx , and deletion of the
NR1 cytoplasmic C - terminal domain , which functions in
Ca ( 2 + ) regulation of receptor currents , decreased
NMDAR mediated cell killing . -
pubmed
These results implicate positive actions of the selectivity filter and of the
NR1 C - terminal domain in a
Ca ( 2 +)-dependent mechanism for NMDAR excitotoxicity . -
pubmed
The abnormality appears to inhibit the binding of
vitamin B6 to the enzyme
glutamic acid decarboxylase-1 , which is needed for the biosynthesis of
gamma - aminobutyric acid (
GABA ) . -
pubmed
Research has shown that the emetic response is linked to
serotonin and the stimulation of the
5 - HT3 receptors located in several areas of the body . -
pubmed
METHODS : cDNA - expressed
CYP isoforms were used to identify the isoforms that are able to mediate the N - dealkylation of
perphenazine , which is considered a major metabolic pathway for the drug . -
pubmed
RESULTS :
CYP isoforms 1A2 ,
3A4 ,
2C8 ,
2C9 ,
2C18 ,
2C19 and
2D6 were able to mediate the N - dealkylation of
perphenazine . -
pubmed
Ketoconazole inhibition of N - dealkylation mediated by a mixed
HLM indicated that
CYP3A4 accounted for about 40 % of
perphenazine N - dealkylation at therapeutically relevant concentrations . -
pubmed
The contribution of the
CYP isoforms 1A2 ,
2C19 and
2D6 amounted to 20 - 25 % each as measured by the percentage inhibition obtained by addition of
furafylline ,
fluvoxamine or
quinidine , respectively . -
pubmed
HLM - mediated N - dealkylation of
perphenazine accounted for 57 % of the total amount of substrate consumed during incubation . -
pubmed
CONCLUSIONS : The present in vitro study suggests that
CYP isoforms 1A2 ,
3A4 ,
2C19 and
2CD6 are primarily involved in the N - dealkylation of
perphenazine . -
pubmed
When
R - HA was administered concomitantly with
Thiop infusion ,
R - HA failed to attenuate the
RNS - induced antidiuretic action and increases in NEOR . -
pubmed
However , in the presence of
pyrilamine ( a selective
H1 - receptor antagonist ) or
cimetidine ( a selective
H2 - receptor antagonist ) infusion ,
R - HA attenuated the
RNS - induced actions , similarly to the case without these antagonists . -
pubmed
With the use of
PKC alpha -- specific inhibitors (
safingol ,
Go6976 ) as well as the
PKC delta -- specific inhibitor
rottlerin , we showed that only
PKC alpha plays a major role in the regulation of
tumor cell migration . -
pubmed
After stimulation with
phorbol ester we observed a translocation and a colocalization of the activated
PKC alpha at the plasma membrane to the surrounding extracellular matrix . -
pubmed
The
AtPLDdelta protein , of 868
amino acids , has a putative catalytic domain and a
C2 domain that is involved in
Ca2 +/phospholipid binding . -
pubmed
The accumulation of
PtdOH in response to
dehydration was significantly suppressed in
AtPLDdelta antisense transgenic plants . -
pubmed
These results suggest that
AtPLDdelta may be involved in
PtdOH accumulation in the
dehydration stress response . -
pubmed
The lines secreted
TPA ,
IL-6 ,
IL-8 and few or no thyroid - related hormones in the culture supernatant . -
pubmed
METHODS : The changes of
calcium channel dynamics in BSMCs of 4
asthma guinea - pig models ( 22 cells ) and 5 normal guinea - pigs ( 25 cells ) , and the inner peak
calcium current in BSMCs of 5
asthma guinea - pig models ( 25 cells ) and 6 normal guinea - pigs ( 24 cells ) were determined by patch -
clamp techniques . -
pubmed
RESULTS : ( 1 ) The open time of
calcium channel in BSMCs of
asthma group was ( 1.70 + /- 0.40 ) ms , 2.32 times as much as that of control group ( 0.70 + /- 0.10 ) ms ; the close time of
calcium channel in BSMCs of
asthma group ( 5.7 + /- 0.6 ) ms , was significantly shorter that that of control group ( 7.9 + /- 0.4 ) ms ; The open probability in
asthma group ( 0.40 + /- 0.10)% was significantly increased than that of control group ( 0.20 + /- 0.20)% , ( P & lt ; 0.01 ) ; ( 2 ) The inner peak
calcium current in BSMCs of
asthma group was ( -54 + /- 23 )
PA , 1.17 times as muth as that of control group ( -25 + /- 8)
PA , ( P & lt ; 0.01 ) . -
pubmed
It was found that
nicotine self - administration profoundly decreased , in a dose - dependent manner , the expression of
PSA -
NCAM in the DG ; a significant effect was observed at all the doses tested ( 0.02 , 0.04 , and 0.08 mg / kg per infusion ) . -
pubmed
Detailed aggregation studies revealed a dose - dependent inhibition of
platelet aggregation with
ristocetin by DXS 5000 , whereas aggregation with
ADP ,
collagen , and
arachidonate was unaffected . -
pubmed
A direct impairment of erythrocyte membrane
MRP by
hypochlorous acid was shown by electrophoresis and immunoblotting ( c(50%)=478+/-36 microM
HOCl ) . -
pubmed
The stoichiometry of the
MRP /
HOCl reaction was 1:1 . -
pubmed
These results demonstrate that
MRP can be one of the cellular targets for the inflammatory mediator
hypochlorous acid . -
pubmed
The results show that
glutaraldehyde combinative solution (
LacZ ) produced the most consistent and reliable results . -
pubmed
Liposem significantly raised
high - density lipoprotein ( HDL )
cholesterol and the
HDL /
low density lipoprotein +
very low - density lipoprotein (
VLDL+
LDL ) ratio . -
pubmed
Finally , early maternal separation failed to alter expression of messenger ribonucleic acids coding for
5 - hydroxytryptamine1A or
alpha1B receptors in the dorsal raphe nucleus as measured by in situ hybridization histochemistry , suggesting that functional changes in receptor sensitivity observed are not due to changes in receptor gene transcription . -
pubmed
Cardiac allograft rejection is a focal
inflammation and soluble markers are released into coronary
sinus (
CS ) . -
pubmed
In conclusion , NA and
5 - HT exerted a similar action on
MN neurons ; in contrast , the effects of
5 - HT on IN and LN were more differentiated than those exerted by NA . -
pubmed
Aging is associated with a rise in intracellular reactive
oxygen species ( ROS ) and a loss of
telomerase reverse transcriptase activity . -
pubmed
Incubation with
H2O2 induced the nuclear export of
telomerase reverse transcriptase (
TERT ) into the cytosol in a
Src - family kinase - dependent manner . -
pubmed
Therefore , we investigated the hypothesis that age - related increase in reactive
oxygen species ( ROS ) may induce the nuclear export of
TERT and contribute to endothelial cell senescence . -
pubmed
Moreover , the induction of premature senescence by low concentrations of
H2O2 was completely blocked with the
Src - family kinase inhibitor
PP2 , suggesting a crucial role for
Src - family kinases in the induction of endothelial cell aging . -
pubmed
Likewise , nuclear export of
TERT protein , loss in the overall
TERT activity , and the onset of replicative senescence were delayed by incubation with
N - acetylcysteine . -
pubmed
The tests were performed at
rest and while exhaling hard through a mouthpiece connected to a
mercury manometer . -
pubmed
Peak
cortisol responses to all
bCRH treatments were greater ( P & lt ; 0.05 ) than those to pretreatment concentrations . -
pubmed
The ratio of mean
cortisol to mean
ACTH for all
bCRH doses tested differed ( P & lt ; 0.05 ) from control values , indicating reactivity of the adrenals . -
pubmed
Pharmacokinetic modeling of species - dependent enhanced bioavailability of
trifluorothymidine by
thymidine phosphorylase inhibitor . -
pubmed
pyruvate kinase ,
alpha - enolase ,
triosephosphate isomerase ) ,
glucose oxidation ( e.g. -
pubmed
Control of
rHuEPO biological activity : the role of
carbohydrate . -
pubmed
Glycosylation analogs with altered N - linked
carbohydrate content were compared with
rHuEPO to elucidate the relationship between
carbohydrate content and activity . -
pubmed
Assays were performed to compare in vitro receptor binding and in vivo activity of
rHuEPO ,
darbepoetin alfa , and analogs . -
pubmed
CONCLUSION :
Carbohydrate increases persistence of
EPO , resulting in a prolonged and increased biological response in vivo , and overcoming reduced receptor - binding activity . -
pubmed
[ Expression , purification , and characterization of single - chain
disulfide - bond Fv ( ScdsFv ) antibody fused with targeted superantigen
SEA ( D227A ) ] . -
pubmed
coli BL21plusS by
IPTG and purified by Q Sepharose
HP column and Hiprep 26/60 Sephacryl S-200
HR column . -
pubmed
Administration of
MK886 , a
5LO - activity inhibitor , or
MK571 , a selective
cysteinyl - LT ( 1 ) receptor antagonist , largely prevented
ventilator - induced lung injury . -
pubmed
The AP maquette variants ,
AP2 and
AP3 , removed
heme - ligating
histidines from the
HP domain and included
heme - ligating
histidines in
LP domains by selecting the b(H )
heme binding sequence from the membrane - spanning d - helix of
respiratory cytochrome bc ( 1 ) . -
pubmed
These represent the first examples of AP maquettes with
heme and
bacteriochlorophyll binding sites located within the
LP domains . -
pubmed
The absence of any other phycoerythrin gene in the fully sequenced genome of this organism , the previous inability to detect a gene product , and the mutation of two out of four
cysteine residues , normally involved in binding chromophores , suggested that
MED4 -
cpeB might not code for a functional protein . -
pubmed
Microsomal
glutathione peroxidase (
GPx ) activity was determined as the consuming rate of
NADPH . -
pubmed
Athletes can return to their normal activity once a successful management strategy has addressed all components of the female athlete
triad while providing adequate
rest . -
pubmed
Assessment of solvent residues accessibility using three Sulfo -
NHS -
biotin reagents in parallel : application to footprint changes of a
methyltransferase upon binding its substrate . -
pubmed
We present a strategy based on usage in parallel of three commercially available reagents (
Sulfo - NHS - biotin , Sulfo -
NHS - LC -
biotin , and Sulfo -
NHS - LC - LC -
biotin ) to efficiently assess the solvent accessibility of
amino acids using MALDI -
TOF mass spectrometry . -
pubmed
Fusing
octreotide tomographic construction with
CT images allowed accurate determination of the area of increased uptake to be in the right transverse abdominal muscle . -
pubmed
With respect to
heme proteins , the corresponding visible
ECD spectra , which probe the chirality of the
heme environment , have been used to explore functionally relevant structural changes in the
heme vicinity . -
pubmed
While the physical basis of the obtained
ECD signal has been analyzed by Woody and co - workers in terms of multiple electronic coupling mechanism between the electronic transitions of the
heme chromophore and of the
protein ( Hsu , M.C. -
pubmed
Inhibition of
heme oxygenase (
HO ) , the rate - limiting enzyme in
heme catabolism , may be an ideal strategy for preventing
neonatal jaundice . -
pubmed
Although natural and synthetic
heme analogs , called
metalloporphyrins ( Mps ) , have been extensively investigated for this purpose , some Mps are phototoxic , affect the activity of other enzymes , or induce
HO-1 transcription - properties that may limit their clinical use . -
pubmed
Another class of compounds ,
imidazole - dioxolanes , has been shown to selectively inhibit the inducible isozyme
HO-1 . -
pubmed
significantly inhibited
HO activity and reduced in vivo
bilirubin production . -
pubmed
We conclude that
AZA effectively inhibits in vivo
HO activity only at a high dose and that it also induces spleen
HO-1 gene transcription . -
pubmed
Acquiring nutrients from the environment is essential for all microbes , and the
ATP - binding cassette (
ABC ) transporters are one of the major routes by which bacteria achieve it . -
pubmed
, but their persistence in fully determining the properties of this system led to the experimental demonstration that
QAC uptake utilizes three different substrate - binding proteins (
SBPs ) , two of which are encoded at remote locations on the genome as ' orphan '
SBPs that are each able to function with a single core
ABC transporter . -
pubmed
Setting time ( ST ) and compressive strength (
CS ) were measured after 1 day , 1 week and 4 weeks in
phosphate buffered saline (
PBS ) solution , and the corresponding microstructure was evaluated using electron microscopy and X - ray diffraction . -
pubmed
Protein C is a
vitamin K - dependent anticoagulant
serine protease zymogen in plasma which upon activation by the
thrombin - thrombomodulin complex down - regulates the coagulation cascade by degrading cofactors Va and VIIIa by limited proteolysis . -
pubmed
Recent structural and mutagenesis data have indicated that basic residues of three exposed surface loops known as 39 - loop (
Lys-37 ,
Lys-38 , and
Lys-39 ) , 60 - loop (
Lys-62 ,
Lys- 63 , and
Arg-67 ) , and 70 - 80 - loop (
Arg-74 ,
Arg-75 , and
Lys-78 ) (
chymotrypsin numbering ) constitute an anion binding exosite in
APC that interacts with the procoagulant cofactors Va and VIIIa in the anticoagulant pathway . -
pubmed
To gain more insight in the role of galactosylation of
lysines in the Gly - X -
Lys repeats of collagenous proteins , we investigated the subcellular localization of
GLT25D1 . -
pubmed
Additionally we examined co - localization of
GLT25D1 with
MBL and
lysyl hydroxylase 3 (
LH3 ,
PLOD3 ) , which is a protein able to catalyze hydroxylation of
lysine residues before they can be glycosylated . -
pubmed
Comparable rescue results were obtained after elevating endogenous
BDNF protein levels in hippocampus with 4 daily injections of a short half - life
ampakine ( positive modulator of
alpha - amino-3 - hydroxy-5 - methyl-4 - isoxazolepropionate [ AMPA ]- type glutamate receptors ) . -
pubmed
The A subunits of
Stx1 (
Stx1A ) and
Stx2 (
Stx2A ) are ribosome inactivating proteins (
RIPs ) that inhibit translation by removing an
adenine from the highly conserved alpha - sarcin ricin loop (
SRL ) of the large rRNA . -
pubmed
However ,
Asn75 and
Tyr77 were more critical for the depurination activity of
Stx2A , while
Arg176 was more critical for the depurination activity of
Stx1A. -
pubmed
Analysis of solvent accessible surface areas indicated that
Asn75 and
Tyr77 are more exposed in
Stx2A , while
Arg176 is more exposed in
Stx1A , indicating that residues with higher surface exposure were more critical for enzymatic activity . -
pubmed
Mental disorders were assessed using the
Alcohol Use Disorder and Associated Disabilities Interview Schedule -
IV version . -
pubmed
At present , the described
mannosidases specific for
alpha1,6 - mannose linkages are very limited in number . -
pubmed
Analysis of
SpGH125 and
CpGH125 reveal them to have
exo - alpha1,6 - mannosidase activity consistent with specificity for
N - linked glycans having their
alpha1,3 - mannose branches removed . -
pubmed
An
EPR study has been carried out to investigate the structure and behaviour of the free radical formed gamma - irradiated
l - alaninamide hydrochloride ,
dl - glutamic acid monohydrate and
N -( 2 - carboxyethyl ) iminodiacetic acid powders at room temperature . -
pubmed
The main target is thereby the early proximal tubule proximal tubule where secondary active transport secondary active transport of the
sugar is mediated by the
sodium - D : - glucose D - glucose cotransporter SGLT2 SGLT2 . -
pubmed
The properties of these binding sites differ between
SGLT2 and
SGLT1 SGLT1 , which mediates
sugar absorption
sugar absorption in the intestine intestine . -
pubmed
Various O- , C- , N- and
S - glucosides have been synthesized with high affinity affinity and high specificity specificity for
SGLT2 SGLT2 . -
pubmed
Fungal
ribotoxins are toxic secreted
ribonucleases that cleave a conserved single
phosphodiester bond located at the sarcin / ricin loop of the larger rRNA . -
pubmed
Although several mediators including reactive
oxygen species ,
platelet derived growth factor ,
transforming growth factor - beta (
TGF - beta ) and
tumour necrosis factor - alpha (
TNF - alpha ) were implicated in HSC activation , the cellular signalling pathways that regulate this process remain incompletely defined . -
pubmed
The
TGF - beta - induced
alpha - SMA expression was blocked by
AACOCF ( 3 ) . -
pubmed
In the current investigation , immunoblot analysis intriguingly demonstrated that
Pfnek3 , a plasmodial
mitogen - activated protein kinase kinase (
MAPKK ) , displayed both
serine /
threonine and
tyrosine kinase activities in autophosphorylation reactions as well as in phosphorylation of the exogenous
myelin basic protein substrate . -
pubmed
Further probing into the regulatory mechanism of
Pfnek3 also revealed
tyrosine phosphorylation to be a crucial factor that stimulates its
kinase activity . -
pubmed
Through biocomputational analyses and functional assays ,
tyrosine residues Y117 , Y122 , Y172 , and Y238 were proposed as phosphorylation sites essential for mediating the catalytic activities of
Pfnek3 . -
pubmed
In addition to the expected HR adaptation , the VR response to
beta - adrenoceptor stimulation with
Iso and to
muscarinic receptor blockade with
atropine thus included alterations previously observed in congenital and acquired
long QT syndromes , demonstrating substantial overlap between physiological and pathophysiological electrophysiology . -
pubmed
Importantly ,
N - acetyl cysteine (
NAC ) treatment effectively blocked apoptosis via the
caspase-9 and
caspase-3 pathways while
NAC attenuated the inhibition of mitochondrial complex I activity as well as the oxidative metabolism of
dopamine ( DA ) . -
pubmed
In the response of cellular antioxidant systems to
CPF , we found that
CPF treatment increased
HO-1 expression while the expression of
CuZnSOD and
MnSOD was reduced . -
pubmed
In addition , we found that
CPF treatment activated
MAPK pathways , including
ERK 1 / 2 , the
JNK , and the
p38 MAP kinase in a time - dependent manner . -
pubmed
NAC treatment abolished
MAPK phosphorylation caused by
CPF , indicating that ROS are upstream signals of
MAPK . -
pubmed
Interestingly ,
MAPK inhibitors abolished
cytotoxicity and reduced ROS generation by
CPF treatment . -
pubmed
Our results demonstrate that
CPF induced neuronal cell
death in part through
MAPK activation via ROS generation , suggesting its potential to generate oxidative stress via
mitochondrial damage and its involvement in oxidative stress - related
neurodegenerative disease . -
pubmed
The aim of this study was to compare the concordance of immunophenotype established with routine cytopathology ( slides stained with Giemsa
solution ) and immunocytochemistry according to
CD3 and
CD79 alpha immunoreactivity . -
pubmed
Cytosolic
phospholipase A2 alpha /
arachidonic acid signaling mediates depolarization - induced suppression of excitation in the cerebellum . -
pubmed
Intracellular
Ca ( 2 + ) elevation is critical for the endocannabinoid production and
DSE . -
pubmed
Nevertheless , how elevated
Ca ( 2 + ) leads to
DSE is unclear . -
pubmed
The degradation enzyme of
2 - arachidonoylglycerol (
2 - AG ) ,
monoacylglycerol lipase (
MAGL ) , blocked
DSE , while another catabolism enzyme for
N - arachidonoylethanolamine (
AEA ) ,
fatty acid amide hydrolase (
FAAH ) , did not affect
DSE . -
pubmed
Co - application of
paxilline reversed the blockade of
DSE by internal K(+ ) , indicating that
large conductance Ca ( 2 +)- activated potassium channel (
BK ) is sufficient to inhibit cPLA(2)alpha /
arachidonic acid - mediated
DSE . -
pubmed
In addition , we showed that the release of
2 - AG was independent of soluble
NSF attachment protein receptor (
SNARE ) ,
protein kinase C and
protein kinase A. -
pubmed
,
rivaroxaban ,
apixaban and
edoxaban ) and
thrombin inhibitors ( e.g. -
pubmed
Despite
coils are mostly used
embolic material in
pseudoaneurysm , embolization with
histoacrylate glue can be highly effective and quick treatment modality for
post - traumatic hepatic artery pseudoaneurysm , especially in cases when it is impossible to use standard materials . -
pubmed
Predicting the highest intensity ion in multiple charging envelopes observed for denatured
proteins during electrospray ionization mass spectrometry by inspection of the
amino acid sequence . -
pubmed
A simple , manual method for predicting the highest intensity charge states ( HICS ) of denatured
protein ions generated by electrospray ionization based on inspection of the
proteins '
amino acid sequence is proposed . -
pubmed
Upon reduction of the
disulfide bonds of these
proteins , the experimental HICS became closer to the predicted values , suggesting that charging lower than the prediction can be attributed to conformational inflexibility of those proteins . -
pubmed
This paper reviews the discovery and history of the use of irreversible
monoamine oxidase (
MAO ) inhibitors ( MAOIs ) such as
phenelzine ,
tranylcypromine and
isocarboxazid , as well as the second generation selective and reversible MAOIs such as the
MAO - A inhibitor ,
moclobemide and the
MAO - B inhibitor ,
selegiline . -
pubmed
CM - HP - beta - CD showed the strongest
insulin - binding ability and the best transport properties compared with the other beta - cyclodextrin derivatives we evaluated . -
pubmed
RESULTS : After PCR correction , ACPR at D28 was 100 % for A - L , 96.55 % for
AQ - SP , and 100 % for AQ -
AS , with no significant difference between the three combinations ( p = 0.36 ) . -
pubmed
Some
NR activity ( 15 % ) could be restored by adding unphysiologically high concentrations of
molybdate to the culture medium in two of the
cnx - lines ( lines 3 and 4 ) . -
pubmed
Could pro - BNP ,
uric acid ,
bilirubin ,
albumin and
transferrin be used in making the distinction between
stroke subtypes ? -
pubmed
We observed significantly higher values within 24 hours of
uric acid ( p & lt ; 0.05 ) , significantly lower values within 24 hours of
transferrin ( p & lt ; 0.05 ) , significantly lower values at 7 days of
albumin and
transferrin ( p & lt ; 0.001 ) , significantly higher values at 7 days of
uric acid and
bilirubin ( p & lt ; 0.001 ) . -
pubmed
( 18 ) FDG - PET and
( 18 ) FDG - PET /
CT might be useful to confirm suspected
pancreatic cancer . -
pubmed
Pooled estimates for
( 18 ) FDG - PET /
CT were : sensitivity 90 % , specificity 76 % , PPV 89 % , NPV 78 % and accuracy 86 % . -
pubmed
CONCLUSION : Both
( 18 ) FDG - PET and
( 18 ) FDG - PET /
CT offer no benefit over the current primary diagnostic tools in diagnosing
pancreatic cancer . -
pubmed
However , the
( 18 ) FDG - PET /
CT systems are still improving . -
pubmed
Yet ,
( 18 ) FDG - PET /
CT may have a role in the staging of
pancreatic cancer , in survival prediction , and may add to other diagnostic information , like histology . -
pubmed
Evaluation of two commercial
omalizumab / free
IgE immunoassays : implications of use during therapy . -
pubmed
The oxidative breakdown of carotenoid evidences the formation of
apocarotenoids through
carotenoid cleavage dioxygenases (
CCDs ) . -
pubmed
Comprehensive identification of
disulfide bonds using non - specific
proteinase K digestion and CID - cleavable crosslinking analysis methodology for Orbitrap LC / ESI -
MS /
MS data . -
pubmed
Here , we describe an updated application of our CID - cleavable crosslink analysis software and our crosslinking analysis with non - specific digestion methodology for the robust and comprehensive determination of
disulfide bonds in proteins , using Orbitrap LC / ESI -
MS /
MS data . -
pubmed
Anthropometric measurements , as well as dietary intake , selected vital signs , resting energy expenditure , blood lipids ,
glucose ,
insulin , and selected adipose - derived hormones were measured at baseline , and weeks 2 , 12 , and 24 . -
pubmed
Both groups experienced significant decreases in
body mass index , fat - free soft tissue mass , body fat percentage , waist and hip circumferences and serum
triglycerides , total
cholesterol , and
leptin ( all P & lt ; 0.001 ) . -
pubmed
PURPOSE : To investigate the association between the single
nucleotide polymorphisms ( SNPs ) of the
adiponectin gene and
nonalcoholic fatty liver disease (
NAFLD ) as well as the impact of the interaction of multiple SNPs on
NAFLD risk , based on a Chinese population study . -
pubmed
Exendin-4 protects HUVECs from
t - BHP - induced apoptosis via
PI3K /
Akt -
Bcl-2 -
caspase-3 signaling . -
pubmed
METHODS : HUVECs were treated with 100 micromol / L
t - BHP for 4 h , following pretreatment with 2.5 - 25 nmol / L
exendin-4 . -
pubmed
The percentage of apoptotic cells was determined by fluorescence microscopy after
Hoechst /
PI staining . -
pubmed
Exendin-4 reversed
t - BHP - mediated inhibition of
Akt phosphorylation , which was abrogated by the
PI3 K inhibitor ,
wortmannin . -
pubmed
Sirtuins (
SIRT1 - 7 ) are a family of
nicotine adenine dinucleotide (
NAD+)-dependent enzymes that catalyze
post - translational modifications of proteins . -
pubmed
Ovalbumin with Glycated
Carboxyl Groups Shows Membrane - Damaging Activity . -
pubmed
Our results show a significant role for RNA transcription : 50 % of upregulated transcription factors were related to
hypoxia responses , differentiated modules were significantly enriched for
oxygen transport , and importantly , divergent
EPAS1 functional variants with a refined co - expression network were identified . -
pubmed
Aminopeptidases are
exopeptidases that catalyze the hydrolysis of
amino acid residues from the N terminus of peptides and proteins . -
pubmed
The complex formation of the ion - pair formed between the anionic chelate of
Ge ( IV )- 4 - nitrocatechol ( 4 -
NC ) and the cation of
1,4 - diphenyl-3 -( phenylamino )- 1H-1,2,4 - triazole (
Nitron ,
Nt ) in the liquid - liquid extraction system
Ge ( IV )- 4 - NC - Nt - H2O - CHCl3 was studied by spectrophotometry . -
pubmed
Herein is reported the design , fabrication , and test of flexible , thin film
silicon photodetectors ( PDs ) bonded to a flexible substrate designed for use in conformal
DRS . -
pubmed
Furthermore , antibiotic resistance phenotypes , including
macrolide and
vancomycin resistance , as well as virulence factor - encoding genes [
asa1 ,
esp - fm ,
esp - fs ,
hyl , and
cyl ( B ) ] were investigated in all isolates . -
pubmed
METHODS AND RESULTS : The association between gestational age ( in weeks ) obtained from Child Health Services records and
CRF , estimated from field and laboratory tests and expressed by maximal
oxygen uptake level through adolescence to young adulthood , was examined in 791 participants in the Northern Ireland Young Hearts Study , all singletons born at term . -
pubmed
In this study , we demonstrated that sustained stimulation of
beta - ARs significantly induced synthesis and secretion of growth factors , including
connective tissue growth factor (
CTGF ) and
vascular endothelial growth factor (
VEGF ) , via the
cAMP - dependent and
protein kinase A (
PKA)-dependent pathways . -
pubmed
In addition ,
isoproterenol (
ISO)-mediated synthesis and secretion of
CTGF and
VEGF through the
beta1 - AR and
beta2 - AR subtypes . -
pubmed
The probability based LiDAR samples capture variations of structure and
carbon across edaphic and climate conditions , and provide an alternative approach to national ground inventory for efficient and precise assessment of forest
carbon resources for emission reduction (
ER ) programs . -
pubmed
The UBLRPA products were identified visually using the PLFD through dual hybridization ( a PNA probe on the gold nanoparticle (
Au -
NP ) was combined with a universal linker on the end of the products ; a PNA capture probe was used on the test line and a universal linker on the other end of the products ) . -
pubmed
The targets of highly connected miRNA ( = 12 ) indicated the significant biological pathways and gene ontology ( GO ) terms , such as
MAPK and Wnt signaling and
magnesium - ion binding , which are associated with apoptotic activities . -
pubmed
We performed histological examination ( with
hematoxylin and eosin staining ) , immunohistochemical examination ( with use monoclonal antibodies
CD90 ( 1:25 ; Abcam , UK ) , SD105 ( 1:500 ; Abcam , UK ) ,
CD44 ( 1:25 ; Dako ) , SD73 ( 1:200 , Abcam , UK ) , and
electron microscopy ( by microscope Philips / FEI Corporation , Eindhoven , Holland ) . -
pubmed
Nongenomic actions of thyroid hormone are initiated by the hormone at receptors in the plasma membrane , in cytoplasm , or in mitochondria and do not require the interaction of
nuclear thyroid hormone receptors (
TRs ) with their primary ligand ,
3,5,3 '- triiodo - l - thyronine ( T3 ) . -
pubmed
Reviewed here are nongenomic effects of the hormone - T3 or , in some cases ,
l - thyroxine ( T4)-that are initiated at ( a ) truncated
TRalpha isoforms , e.g. -
pubmed
Loaded
KOH and/or
Ba ( OH ) 2 were identified by SEM - EDX and BET analysis , respectively , and the
CO2 adsorption performances were confirmed using a gas flow system with
GC . -
pubmed
The T7
RNA polymerase / T7 promoter overexpression system , which is an
isopropyl - beta - d - thiogalactopyranoside (
IPTG)-inducible gene overexpression system ( T7 - dependent inducible overexpression system ) , was modified to be a constitutive overexpression system . -
pubmed
The F1.5 scores of the first 12 key indicators peaked at iteration ( 0.893 ) , including
hemoglobin (
Hb ) ,
calcium ( CA ) , red blood cell count ( RBC ) , mean platelet volume ( MPV ) , mean erythrocyte
hemoglobin concentration ( MCH ) ,
systolic blood pressure (
SBP ) , platelet count ( PLT ) ,
magnesium ( MG ) , lymphocyte ( LYM ) ,
glucose ( GLU , blood gas analysis ) ,
glucose ( GLU , blood biochemistry ) and diastolic blood pressure (
DBP ) . -
pubmed
This effect was reversed by the
cannabinoid 1 ( CB1 ) receptor antagonist ,
AM251 ( 1 mg / kg , ip ) , but not the
PPARalpha antagonist ,
MK886 ( 1 mg / kg , ip ) . -
pubmed
MWD did not modify levels of the endocannabinoids
2 - AG and
AEA , nor those of the
PPARalpha ligands ,
OEA and
PEA , in any region evaluated . -
pubmed
Spiperone elicited inverse agonist inhibitory sigmoid isotherms in all membrane preparations while
WAY100635 was mostly ' silent ' for
Galphai3 activation . -
pubmed
Divergent roles of Kupffer cell
TLR2 / 3 signaling in
alcoholic liver disease and the protective role of
EGCG . -
pubmed
We also identified a novel
ALD - protective agent
EGCG which directly interacted with Kupffer cell
TLR2 / 3 to induce
IL-10 production . -
pubmed
Deficiency of
IL-10 aggravated
ALD injury and blunted
EGCG - mediated hepatoprotection while depletion of Kupffer cells partially recovered
liver injury but abolished
EGCG 's actions . -
pubmed
A 3 months 40 mg
pantoprazole therapy was ineffective in reduction of
VP complications in our study group . -
pubmed
Treatment with a low - dose combination of one antiandrogen and antimineralocorticoid drug (
spironolactone ) and two
insulin sensitizers (
pioglitazone /
metformin ) ( SPIOMET ) is particularly effective in improving these metabolic derangements . -
pubmed
This is consistent with the effects of
pioglitazone and
spironolactone inducing
CXCL14 expression and promoting a brown - like phenotype in adipocytes . -
pubmed
Infarct size was not significantly different between the two groups ; however , the
DFP group demonstrated earlier resolution of
hemorrhage ( by
T2 * imaging ) and
edema ( by
T2 imaging ) . -
pubmed
Squalene and
cholesterol in the balance at the
ER membrane . -
pubmed
The treatments were as follows : 0 , 25 , 50 , and 75 mg / kg / day of
cadmium chloride (
CdCl2 ) by oral gavage from
PND 21 to observation of first vaginal opening ( VO ) . -
pubmed
Holstein heifers ( n = 51 ; 10.5 to 14.5 mo , and 325.0 + - 21.1 kg of
body weight ) were superovulated ( n = 69 events ) for the collection of embryos using a protocol based on sequential administration of
FSH for follicle superstimulation and
GnRH to induce ovulation . -
pubmed
A synthesized
thio - anthraquinone analogue ( 3 ) was purified by column chromatography , and its chemical structure was characterized by spectroscopic methods such as Fourier - transform infrared spectroscopy ( FT - IR ) , nuclear magnetic resonance ( NMR ) spectroscopy , mass spectrometry (
MS ) , and ultraviolet ( UV)-visible spectrophotometry . -
pubmed
However , after the reaction with
H2O2 and the 30 mM
trisodium citrate dihydrate mixture in the presence of an
iron sulfate (
II ) solution , a single peak was observed , producing a stable and reddish / brownish homogenous solution ( lambda max = 304 nm ) . -
pubmed
METHODS : Part 1 : An in vitro catalytic reaction comprising the
ppGalNAc T2 enzyme ,
uridine-5 '- diphospho - N - galactosamine (
UDP - GalNAc ) and an
11 - amino acid peptide substrate , was used to assess the enzyme activity of the
ppGalNAc T2 enzyme in macromolecular crowding environment respectively with
bovine serum albumin (
BSA ) ,
polyethylene glycol (
PEG2000 ) ,
Dextran70 and
Ficoll70 at different concentration and temperature . -
pubmed
Macromolecular crowding was simulated using
PEG2000 at the concentrations of 50 , 100 , and 200 g / L. Deglycosylated -
MUC5AC ( d -
MUC5AC ) and glycosylated
MUC5AC ( g -
MUC5AC ) were denatured by
GdnHCl and renatured by dilution in a refolding buffer . -
pubmed
RESULTS :
Enzyme activityof
ppGalNAc T2 was observed to increase with increasing crowding agent concentration , with highest
enzyme activity at 200 g / L. Compared with the group in the absence of crowding reagent , the refolding yield of
g - MUC5AC and
d - MUC5AC were reduced significantly in the presence of different concentrations of
PEG2000 ( 200 , 100 , and 50 g / L ) . -
pubmed
Compared with the dilute solution , aggregation increased significantly in the presence of
PEG2000 , especially at 200 g / L. Moreover , in the crowded reagent with the same concentration , the refolding yield of
d - MUC5AC was higher than that of
g - MUC5AC , whereas the degree of aggregation of
d - MUC5AC was lower than that of
g - MUC5AC . -
pubmed
Chitinases play an important role in many industrial processes , including the preparation of
oligosaccharides with potential applications . -
pubmed
The hydrolytic products derived from colloidal chitins comprised mainly
( GlcNAc ) 2 and
GlcNAc , indicating that rChi1602 is a GH18 processive
chitinase in conformity with its hypothetical structure . -
pubmed
However ,
rChi1602 showed traces of
beta - N - acetylglucosaminidase activity on substrates such as powder chitin , chitosan , and
ethylene glycol chitin . -
pubmed
Myelin Oligodendrocyte Glycoprotein Antibody Disease (
MOGAD ) represents a
demyelinating disorder for which
tocilizumab , an
anti - IL6 receptor , has been tested to prevent disabling
relapses . -
pubmed
The
enzyme can utilize the A - particle endogenous RNA as template as shown by analysis of the early and late DNA products of the endogenous reaction by
CsSO4 isopycnic gradient centrifuation and hybridization of purified 70S or
35S A - particle RNA with the purified complementary DNA product . -
pubmed
Only one infant developed
HMD out of 170 with
amniotic fluid PAPase specific activity equal to or greater than 50 nmoles of
orthophosphate released X mg.-1 of
protein X hr. - pubmed
A similar binding pattern is produced in control membranes by treatment with
Triton X-100 ,
phospholipase C , or
glycerophosphoethanolamine . -
pubmed
Substrate activity of
( adenosine triphosphato ) tetraamminecobalt ( III ) with
yeast hexokinase and separation of diastereomers using the enzyme . -
pubmed
When an efficient
SOD mimic operating in vitro is introduced into cells , the following requirements are needed in order that this compound will catalyze
O2- dismutation efficiently : it should be non toxic , stable , has a long metabolic half life , does not form ternary complexes with the cell components , its reduced form reacts slowly with molecular
oxygen , should be able to cross cell membranes and also to reach lipophilic or hydrophobic regions . -
pubmed
The mean proportion of
bilirubin removed by routine plasma exchange was 29 % with a mean of plasma exchange volume of 2.1 L. In the plasma perfusion with Bespore and
BR , the removal rates were 37 % and 42 % , respectively . -
pubmed
From the results of
tunicamycin treatment and
N - glycosidase F digestion , it was demonstrated that Namalwa - derived
hGM - CSF was highly glycosylated at two potential N - glycosylation sites and several O - glycosylation sites as previously shown for naturally occurring
hGM - CSF . -
pubmed
To investigate the role of
carbohydrate moieties of
hGM - CSF , we isolated each form of
hGM - CSF and examined its biological properties . -
pubmed
From these findings , we concluded that N - linked
carbohydrate moieties of
hGM - CSF play conflicting physiological roles in the efficacy of the protein in vivo but that O - linked
carbohydrate moieties do not have such effects . -
pubmed
Optimization of techniques in screening
CT of the
sinuses . -
pubmed
The number of screening examinations of the
sinuses performed with
CT has markedly increased owing to the widespread and increasing use of endoscopic sinonasal surgery . -
pubmed
Three aspects of direct coronal imaging of the paranasal
sinuses were investigated : ( 1 ) preparation of the patient prior to the examination ; ( 2 ) technical factors of the
CT study , including positioning of the patient , optimal coronal angle , slice thickness , and
CT exposure factors ; and ( 3 ) data display . -
pubmed
Forskolin is often used to activate
adenylate cyclase in studies relating
adenosine 3 ' , 5 '- cyclic monophosphate (
cAMP ) to the modulation of membrane current . -
pubmed
Furthermore , both IA and IK.V were reduced by
1,9 - dideoxy - forskolin , a derivative of
forskolin that does not activate
adenylate cyclase . -
pubmed
Bath application of a modified form of
forskolin (
7 - deacetyl-6 -[ N - acetylglycyl ]- forskolin ) , which has enhanced water solubility and activates
adenylate cyclase , reduced IK.S , but did not alter either IA or IK.V. -
pubmed
Thus it appears that certain derivatives of
forskolin can be used to activate
adenylate cyclase and avoid some of the nonspecific actions on membrane current that are associated with
forskolin . -
pubmed
During early follicular development ,
FSH binds to granulosa cells of primary follicles to stimulate production of
estradiol by the induction or enhancement of
aromatase synthetase ( 37 , 336 , 337 ) . -
pubmed
Estradiol , in turn , induces proliferation of granulosa cells ( 338 - 344 ) and increases the sensitivity of the follicle to further
gonadotropin stimulation ( 12 , 339 , 345 - 349 ) . -
pubmed
Estradiol can synergize with
gonadotropins to increase ovarian weight , enhance proliferation of granulosa cells , and promote growth of preantral follicles and antrum formation ( 345 , 347 , 350 - 352 ) . -
pubmed
In addition ,
estradiol enhanced the responsiveness of granulosa cells to
FSH and
LH by increasing synthesis of
progesterone ( 353 , 354 ) . -
pubmed
The generalized enhancement of
gonadotropin action by
estradiol is partially mediated by
FSH - induced accumulation of
cAMP . -
pubmed
Estradiol not only enhances
gonadotropin stimulation of
LH and FSH receptors in granulosa cells ( 357 , 348 ) but is required for
FSH induction of
FSH receptors ( 359 , 360 ) . -
pubmed
Estradiol alone can increase numbers of its own receptor in granulosa cells ( 350 ) as well as increase its own production by stimulating
aromatase activity ( 361 ) . -
pubmed
Estradiol secreted by the dominant follicle has a positive feedback effect on the hypothalamus and pituitary , enhancing
gonadotropin secretion and ensuring the preovulatory
gonadotropin surges ( 362 ) . -
pubmed
The increased
gonadotropins can further increase the production of
estradiol which , in turn , enhances its own production . -
pubmed
A
compartment syndrome developed the day after heparinization and was adequately treated by discontinuation of
heparin , aspiration and
rest . -
pubmed
N - ethoxycarbonyl-2 - ethoxy-1,2 - dihydroquinoline ,
amiloride analogues , and renal
Na +/ H + antiporter . -
pubmed
N - ethoxycarbonyl-2 - ethoxy-1,2 - dihydroquinoline (
EEDQ ) is a
carboxyl - activating agent and has been shown to inhibit the renal
Na +/ H + antiporter . -
pubmed
The purposes of the present studies were to characterize the kinetics of inhibition of the
Na +/ H + antiporter by
EEDQ and to determine whether
amiloride analogues affect the ability of
EEDQ to inhibit the rate of
Na+/
H+ exchange . -
pubmed
Cyclosporine functions in the early sequence of T cell activation by regulating the production of messenger RNA for
lymphokines synthesis . -
pubmed
Cyclosporine does not inhibit the synthesis of
lymphokine once the
lymphokine message is present in the cell . -
pubmed
Prevention of
post - tonsillectomy
pain with analgesic doses of
ketamine . -
pubmed
In serum - free medium the
monoclonal antibody produced a 45 % reduction of cells in S phase by
thymidine labeling index without modification of the growth fraction as determined by primer - dependent
alpha - DNA polymerase labeling index . -
pubmed
They have been identified as
1 - monomethylphosphoinositol 4,5 - bisphosphate and
1 - monomethylphosphoinositol 4 - phosphate by comparison to a synthesized standard using HPLC chromatography , paper electrophoresis , and enzymatic dephosphorylation with
inositol polyphosphate 5 - phosphomonoesterase and
intestinal alkaline phosphatase [
orthophosphoric - monoester phosphohydrolase (
alkaline optimum ) ,
EC 3.1.3.1 ] . -
pubmed
[ Effect of
actinomycin D on the stimulation of
water and
sodium transport with
antidiuretic hormone ] . -
pubmed
Rat liver phenylalanine hydroxylase , an
iron enzyme . -
pubmed
Dopamine and noradrenaline receptor stimulation : reversal of
reserpine - induced suppression of motor activity . -
pubmed
What
lectins and
carbohydrates are made for ? -
pubmed
The
nucleotide sequence of
rabbit embryonic globin gene beta 3 . -
pubmed
Increase of
L - lysine concentration in the luminal perfusate to 20 mM did not reduce
JHCO3 further nor did it influence Jv.l When 10 mM
L - lysine was added to the capillary perfusate , a 13 % reduction in
JHCO3 was observed (
NS ) . -
pubmed
The monolayer culture was used in morphologic , cytochemical and cytoenzymatic studies , and the culture medium was used for assay of
thyroglobulin and
thyroxin . -
pubmed
We found that addition of
aminoacids with
SH or
S -- S groups to the culture medium causes stimulation of protein synthesis overstrain on the
oxydases system at the expense of
dehydrogenases . -
pubmed
These included ultraviolet ( UV ) absorption , isoelectric points ,
sulfhydryl content , extinction coefficients , and circular dichroism (
CD ) . -
pubmed
The effect of
glycerol on the conformation of
sigma , core , and
holoenzyme was monitored by circular dichroism measurements . -
pubmed
In the far - ultraviolet , the residue ellipticity at 220 nm ( [ theta]220 ) increased approximately 15 % from 0 to 10 %
glycerol for both core and
holoenzyme . -
pubmed
In the near - ultraviolet , the residue ellipticity at a peak near 280 nm also varied with
glycerol concentration , decreasing in intensity by about 50 % with
holoenzyme , when
glycerol was raised from 5 to 10 % , then increasing at still higher
glycerol contents . -
pubmed
The presence of 10 %
glycerol in the assay buffer increased the activity of the
enzyme . -
pubmed
Development of dyes that can be exited at 488 nm and have emission maximum in the far red area has made three - colour protocols , together with
fluorescein isothiocyanate (
FITC ) and
R - phycoerythrin (
PE ) , possible in most clinical flow cytometers . -
pubmed
Neuroendocrine and haemodynamic responses to abdominal aortic cross
clamp and release during high - dose opiate -
oxygen -
isoflurane anaesthesia . -
pubmed
FSH - induced
estradiol production by granulosa cells from large follicles ( expressed as pg
estradiol/10(5 ) cells/24 h ) was not affected ( p & gt ; 0.05 ) by 0.1 , 1.0 , or 10 ng / ml of
interleukin ( IL )- 6 , whereas 30 and 100 ng / ml of
IL-6 inhibited ( p & lt ; 0.05 )
FSH - induced
estradiol production by 32 % in cells from large follicles . -
pubmed
In comparison , all doses of
IL-6 inhibited ( p & lt ; 0.05 )
FSH - induced
estradiol production in cultures of cells from small follicles ; maximal inhibition was achieved ( 75 % decrease ) with & gt ; or = 10 ng / ml . -
pubmed
Ribonucleotide reductase is expressed in actively dividing cells and generates
deoxyribonucleotides for DNA synthesis . -
pubmed
These sera recognized different
P450IID6 peptide sequences consisting of
amino acids ( aa ) 200 - 214 and aa 271 - 339 . -
pubmed
Discovery of potent
isoxazoline glycoprotein IIb / IIIa receptor antagonists . -
pubmed
Using the
isoxazoline as a common structural feature , three series of
glycoprotein IIb / IIIa receptor antagonists were evaluated , culminating in the discovery of
XR299 ( 30 ) . -
pubmed
Nicks generated by the enzyme were resealable with
T4 DNA ligase , indicating that the enzyme produces 5'-p and 3'-
OH ends . -
pubmed
Influence of
n-3 fatty acid supplementation on the endogenous activities of
plasma lipases . -
pubmed
Low - density - lipoprotein -
cholesterol concentrations increased by 25 % in the latter group ( P = 0.06 ) . -
pubmed
An SH3 - domain - containing protein has been identified that interacts with sequences in the
cABL carboxyl terminus . -
pubmed
Additionally , these results provide further evidence for the importance of the
cABL carboxyl terminus and its binding proteins in the regulation of
cABL function . -
pubmed
The gene product of the
yeast VPS1 gene is a member of a family of high - molecular - weight
GTP - binding proteins that are involved in diverse cellular processes . -
pubmed
DNA sequence analysis of the mutant
vps1 allele (
vps1d-293 ) revealed a single point mutation , resulting in an
amino acid exchange at position 293 from
Ala to
Asp . -
pubmed
Following earlier work on
cystine - bridged peptides , cyclic phosphopeptides containing nonreducible mimics of
cystine were synthesized that show high affinity and specificity toward the
Src homology ( SH2 ) domain of the
growth factor receptor - binding protein (
Grb2 ) . -
pubmed
The
enzyme was solubilized by detergent treatment ( 2 % [ w / v ]
Triton X-100 ) and purified by
heparin - Sepharose and Q - Sepharose chromatography . -
pubmed
While
Mg ( 2 + ) was the preferred divalent cation ,
Mn ( 2 + ) could partly substitute in the reaction catalyzed by the QII
enzyme but not in that catalyzed by QI . -
pubmed
Mn ( 2 + ) acted synergistically with suboptimal
Mg ( 2 + ) concentrations to activate not only the
QII enzyme , but also to some extent QI . -
pubmed
Both
enzymes were inhibited by millimolar concentrations of
Ca ( 2 + ) and micromolar concentrations of
wortmannin . -
pubmed
The apparent molecular mass for QI was 120 kD , which was determined by
SDS - PAGE and western blotting using an antibody against a peptide unique for
lipid kinases and the binding of
( 3 ) H - wortmannin , and for QII 65 kD as determined by immunodetection and renaturation of
PtdIns kinase activity in the 65 - kD region of
polyacrylamide gels . -
pubmed
Modulation of apoptotic and inflammatory genes by
bioflavonoids and
angiotensin II inhibition in
ureteral obstruction . -
pubmed
We investigated the effect of
bioflavonoids and
angiotensin II inhibition on apoptotic and inflammatory gene expression in a model of
unilateral ureteral obstruction (
UUO ) . -
pubmed
Treatment with
curcumin increased
HO-1 expression fourfold ( P & lt;0.05 ) . -
pubmed
Bioflavonoids and
angiotensin inhibitors are able to attenuate the expression of these genes and thus may be beneficial in renal protection . -
pubmed
Trace - level determination of
1,4 - dioxane in water by isotopic dilution
GC and
GC -
MS . -
pubmed
This investigation established that
1,4 - dioxane can be determined in water by various techniques including direct aqueous injection (
DAI ) gas chromatography (
GC ) and purge and trap
GC - mass spectrometry (
MS ) . -
pubmed
Purge and trap
GC -
MS is limited by
1,4 - dioxane 's poor purge efficiency , resulting in detection limits up to 100 times greater than the efficiently purged volatile organic compounds . -
pubmed
Isotopic dilution quantification was also possible using a non - selective
GC detector owing to the high efficiency of capillary
GC columns that resolve the
deuterium - labeled solvent from the natural isotopes . -
pubmed
The ligated
benzonitriles in the
platinum ( II ) complex
[ PtCl2 ( PhCN ) 2 ] undergo metal - mediated [ 2 + 3 ] cycloaddition with
nitrones - ON +( R3 )= C ( R1 )( R2 ) [
R1 /
R2 /
R3 =
H / Ph / Me , H / p - MeC6H4 / Me , H / Ph / CH2Ph ] to give delta 4 - 1,2,4 - oxadiazoline complexes , [ PtCl2(N = C(Ph)O - N(R3)-C(R1)(R2))2 ] ( 2a , 4a , 6a ) , as a 1:1 mixture of two diastereoisomers , in 60 - 75 % yields , while [ PtCl2(MeCN)2 ] is inactive toward the addition . -
pubmed
METHODS : The Fluent 4.4.7 code was run on a
Silicon Graphic Inc. computer ( 4 - CPUx185 MHz ) in the
CFD simulation . -
pubmed
Shifting from
E1 to
E2 form is induced by replacing
Na + by K+ ions . -
pubmed
The detection threshold allowed the possibility to detect a change affecting five
amino acids out of the 1324 that compose the
H +/ K +- ATPase molecule . -
pubmed
Evidence that conformational changes occur in an identical system was brought by monitoring the fluorescence of
fluorescein isothiocyanate - labeled
H +/ K +- ATPase in similar conditions . -
pubmed
In addition to the expected
post - transplantation and
hematologic malignancy cases , other risk groups identified included those with chronic
asthma and
steroid use ,
acquired immunodeficiency syndrome ,
thermal burn ,
hepatic failure , and
postoperative infection . -
pubmed
The complication group showed a smaller
HPD ( P = 0.022 ) , reduced pupillary responses in the
cocaine ( P = 0.037 ) and
pholedrine tests ( P & lt ; 0.001 ) , and anisocor pupil sizes after instillation of the eye drops ( P = 0.034 ) . -
pubmed
Oxygen consumption ( VO2 ) ,
carbon dioxide production ( VCO2 ) , and respiratory exchange ratio (
RER ) were measured at 15 - min intervals . -
pubmed
Compared to the CON diet ,
FAT diet consumption for 5 - 10 weeks was associated with an altered metabolic response to low - intensity exercise , as evidenced by a more than 30 % reduction in the production and utilisation of
glucose ; a decrease in
RER ; a decrease in the estimated rate of whole - body
carbohydrate utilisation ; and an increase in the whole - body rate of lipid oxidation during exercise . -
pubmed
Subsequently , we found
AmpG to be a
permease required for recycling of murein tripeptide and uptake of
anhydro - muropeptides . -
pubmed
These unique substrates for
AmpG , which contain murein peptides linked to
GlcNAc - anhMurNAc , are produced by turnover of the cell wall during logarithmic growth . -
pubmed
AmpG permease is sensitive to
carbonylcyanide m - chlorophenylhydrazone , demonstrating that
AmpG permease is a single - component permease and that transport is dependent on the proton motive force . -
pubmed
The
leucine 7 to
proline 7 (
Leu7Pro ) polymorphism in the signal peptide of
prepro - NPY is associated with increased blood lipid levels , accelerated
atherosclerosis , and
diabetic retinopathy . -
pubmed
Subjects with the Leu7Pro polymorphism had significantly lower plasma
NPY and
norepinephrine concentrations , lower
insulin concentrations , higher
glucose concentrations , and lower
insulin -
glucose ratio in plasma than the controls . -
pubmed
Systolic and diastolic blood pressure , serum free
fatty acid and plasma
leptin ,
ACTH ,
cortisol ,
LH ,
FSH , TSH , free
thyroxin , and
melatonin concentrations were similar during the 24 - h period , compared with controls . -
pubmed
Thioredoxin 2 , an oxidative stress - induced protein , contains a high affinity
zinc binding site . -
pubmed
Both
thioredoxins are capable of reducing
disulfide bonds using a conserved pair of
cysteine residues present in a WCGPC motif . -
pubmed
We show here that
Trx2 contains
zinc in a 1:1 stoichiometry , making it the first identified
zinc - binding
thioredoxin . -
pubmed
The
zinc center of
Trx2 binds
zinc with a very high affinity ( Ka of & gt;1018 m-1 ) . -
pubmed
The
zinc - free protein conserves its
reductase activity . -
pubmed
Previous studies have focused mainly on elucidating the cellular mechanisms responsible for membrane recycling under conditions similar to the stress - activated state and indicate a
clathrin /
dephosphin - mediated retrieval via coated pits . -
pubmed
We show that block of the
calcium - mediated
protein phosphatase 2B , a common step in
clathrin - mediated processes , did not alter endocytosis elicited at basal firing levels . -
pubmed
After
HP test results were confirmed to be positive , anti - HP therapy consisting of
omeprazole ,
clarithromycin , and
amoxicillin was administered for 2 weeks . -
pubmed
2,6 - Pyridinedicarboxylic acid (
PDC ) and
n - hexadecyltrimethylammonium hydroxide (
CTAOH ) were selected to compose a background
electrolyte ( BGE ) , where
PDC was used as chromophore and
CTAOH functioned as electroosmotic flow ( EOF ) modifier to reduce / eliminate EOF . -
pubmed
Beryllium - induced
tumor necrosis factor - alpha production by
CD4 + T cells is mediated by
HLA - DP . -
pubmed
B7 - 1 and
B7 - 2 are closely related
molecules expressed on antigen - presenting cells that provide costimulatory signals for T - cell activation . -
pubmed
Halogenated aromatic hydrocarbons (
HAHs ) , such as
2,3,7,8 - tetrachlorodibenzo - p - dioxin (
TCDD ) , produce a wide variety of biological and toxic effects mainly through the
aryl hydrocarbon receptor (
AhR)-dependent mechanism . -
pubmed
After the binding of
HAHs , the
AhR subsequently transforms its form in order to interact with a specific DNA sequence , the
dioxin responsive element ( DRE ) . -
pubmed
In this study , we have developed a simple method for quantitative assessment of the transformation state of
AhR based on an enzyme - linked immunosorbent assay ( ELISA ) combined with southwestern chemistry technique ( SW - ELISA ) that detects the complex of transformed
AhR :
fluorescein isothiocyanate (
FITC)-labeled DRE probe . -
pubmed
Furthermore , SW - ELISA provides the confirmation that
flavonoids , the potent antagonists for
AhR as reported previously , show the inhibitory effects on
TCDD - induced
AhR transformation . -
pubmed
The model is consistent with current biophysical , biochemical , and structural data ( notably , mass - per - unit - length measurements by scanning transmission electron microscopy that gave one subunit rise per 0.47 nm ) and is readily adaptable to other amyloids , for instance the core of
Sup35p filaments and
glutamine expansions in
huntingtin . -
pubmed
EPR study has shown that the anticancer agent
vanadocene dichloride (
Cp2VCl2 ) interacts with
carbonate contained in physiological solutions . -
pubmed
Both of the expression systems produced alpha - apoprotein in a fully functional form as can judged by its ability to form a structural subunit with native
beta - apoprotein and the pigment molecule
bacteriochlorophyll a. -
pubmed
Although
CT or MRI findings of
adrenal histoplasmosis have been documented , there are no reports regarding
18F - fluorodeoxyglocose (
FDG ) positron emission tomography ( PET ) findings . -
pubmed
The deduced
amino acid sequences encoded by the cDNAs contained characteristic conserved elements of vacuolar
invertases , including the sequence R[G / A / P]xxxGVS[E / D / M]K[S / T / A / R ] , located in the prepeptide region , Wxxx[M / I / V]
LxWQ , located around the starting site of the mature protein , and an intact
beta - fructosidase motif . -
pubmed
Chemical double mutant cycles have been used to measure the effect of a range of substituents on
face - to -
face stacking interactions with
phenyl and
pentafluorophenyl rings . -
pubmed
Previous data show that flow stimulates
SHP2 translocation to cell membranes and binding to
phosphotyrosine proteins . -
pubmed
Flow - induced
ERK1 / 2 phosphorylation depends on
SHP2 phosphatase activity and
SHP2 binding to
phospho -
PECAM1 (
platelet endothelial adhesion molecule 1 ) , suggesting that
SHP2 forms a signaling module with
PECAM1 . -
pubmed
SHP2 - associated proteins were analyzed by
SDS - PAGE and identified by mass spectrometry . -
pubmed
Flow significantly increased
tyrosine phosphorylation of both
Tie2 and
PECAM1 and their association with
SHP2 . -
pubmed
The results show that ACTH , but not
corticosterone , responses to novel restraint are lower in chronically cold stressed females compared to non - stressed control females but no effect of
5 - HT-3A receptor deletion was observed . -
pubmed
The absence of the
PsaC subunit in the
photosystem I ( PSI ) complex ( native PSI complex ) by mutagenesis or chemical manipulation yields a PSI core ( P700 - F(X ) core ) that also lacks subunits
PsaD and
PsaE and the two
iron - sulfur clusters F(A ) and F(B ) , which constitute an integral part of
PsaC. -
pubmed
Sphingosylphosphorylcholine and
lysophosphatidylcholine are ligands for the G protein - coupled receptor
GPR4 . -
pubmed
Although protein expression did not consistently match mRNA levels , a role for the two cell cycle regulators in the
IFN - alpha signaling pathway is suggested as well as a relation with the resistance to
IFN - alpha or
imatinib therapy . -
pubmed
Laboratory findings showed
renal dysfunction (
creatinine clearance , 52 ml / min ) , a moderate range of urinary protein ( 1.51 g / day ) , and increased
serum lysozymes ( 20.7 microg / ml ; normal range , 3.4 - 8.6 microg / ml ) . -
pubmed
Renal biopsy revealed
immune complex glomerulonephritis (
IgM deposition type ) with a membranoproliferative pattern , without
granuloma or
calcium deposition . -
pubmed
The mechanism of DNA cytosine-5 - methylation catalyzed by the bacterial
M.HhaI enzyme has been considered as a stepwise nucleophilic addition of
Cys-81 - S- to
cytosine C6 followed by
C5 nucleophilic replacement of the
methyl of
S - adenosyl - L - methionine to produce
5 - methyl-6 - Cys-81 - S-5,6 - dihydrocytosine . -
pubmed
The pH - dependent titration of wild - type reveals a spectrum characteristic of
family I aminotransferases with an
aldimine pK(a ) of 7.22 . -
pubmed
I249A mutant
hTATase exhibits an unusual spectrum with a similar
aldimine pK(a ) ( 6.85 ) . -
pubmed
hTATase has very narrow substrate specificity with the highest enzymatic activity for the
Tyr /
alpha - ketoglutarate substrate pair , which gives a steady state k(cat ) value of 83 s(-1 ) . -
pubmed
The present findings show that
hTATase is the only known
aminotransferase that discriminates significantly between
Tyr and
Phe : the k(cat)/K(m ) value for
Tyr is about four orders of magnitude greater than that for
Phe . -
pubmed
A comparison of substrate specificities of representative
Ialpha and Igamma aminotransferases is described along with the physiological significance of the discrimination between
Tyr and
Phe by
hTATase as applied to the understanding of the molecular basis of
phenylketonuria . -
pubmed
Acetylcholinesterase inhibitors for
Alzheimer ' s disease : anti - inflammatories in
acetylcholine clothing ! -
pubmed
These observations are supported by evidence showing a role for
acetylcholine in suppression of
cytokine release through a ' cholinergic anti - inflammatory pathway ' . -
pubmed
Taken together , these results indicate that
inulin intake ameliorates
PB - induced
liver injury , associated with a decline in lipid accumulation and
PB - induced expression of
CYP2B and
CYP3A , which may be related by a reduction in the nuclear expression of
HNF4alpha . -
pubmed
The surface properties of treated
ITO substrates were studied by atomic force microscopy (
AFM ) , X - ray photoelectron spectroscopy ( XPS ) , sheet resistance , contact angle , and surface energy measurements . -
pubmed
Osteopontin protects the islets and beta - cells from
interleukin-1 beta - mediated
cytotoxicity through negative feedback regulation of
nitric oxide . -
pubmed
Osteopontin (
OPN ) , a phosphorylated glycoprotein that binds to an integrin - binding motif , has been shown to regulate
nitric oxide (
NO ) production via inhibition of induced
NO synthase (
iNOS ) synthesis . -
pubmed
In the transplanted islets ,
iNOS and toxic amounts of
NO are produced as a result of islets infiltration with inflammatory cells and production of
proinflammatory cytokines . -
pubmed
OPN promoter activity was increased in the presence of
IL-1beta ,
IL-1beta - induced
NO , and an inducer of
NO synthesis . -
pubmed
These data suggest the presence of a cross talk between the
IL-1beta and
OPN pathways and a unique trans - regulatory mechanism in which
IL-1beta - induced
NO synthesis feedback regulates itself through up - regulation of
OPN gene transcription . -
pubmed
OBJECTIVES : We investigated the effects of the orally bioavailable non - immunosuppressive immunophilin ligand
GPI 1046 (
GPI ) on erectile function and cavernous nerve ( CN ) histology following unilateral or bilateral
crush injury ( UCI , BCI , respectively ) of the CNs . -
pubmed
Intra - operative quick
insulin assay to confirm complete resection of
insulinomas guided by selective arterial
calcium injection ( SACI ) . -
pubmed
PMA and
cytokines stimulate binding of
C - Jun ,
C - Fos ,
CCAAT / enhancer binding protein beta (
C / EBPbeta ) , or
NF - kappaB to their respective regulatory elements on
COX-2 promoter . -
pubmed
To further investigate the cellular effects of these drugs in the context of bone healing , we examined the effects of
COX-1 inhibitor
indomethacin and
COX-2 inhibitors ,
parecoxib and
NS398 on osteoclast and osteoblast differentiation and activity in vitro . -
pubmed
We discovered that all tested
COX - inhibitors significantly inhibited osteoclast differentiation , by 93 % , 94 % and 74 % of control for 100 microM
indomethacin , 100 microM
parecoxib and 3 microM
NS398 , respectively . -
pubmed
As this was an integrated reactor / analyzer , the
GC column was exposed to temperatures beyond the degradation point of the column , and so selective ion storage RF waveform was used to remove the
siloxane masses from the spectra . -
pubmed
OBJECTIVES : To determine if
ghrelin and adipocytokine (
leptin ,
adiponectin ,
resistin ) levels vary with menopause stage or with
estradiol (
E2 ) ,
testosterone ( T ) ,
follicle - stimulating hormone (
FSH ) and
sex hormone - binding globulin (
SHBG ) concentrations measured in three stages of the menopause transition . -
pubmed
Downregulation of
BST-2 requires both the transmembrane /
ion channel domain and conserved
serines in the cytoplasmic domain of
Vpu . -
pubmed
Treatment with
SN-38 for 24 h suppressed
TS protein expression and this low level of
TS was maintained for at least 72 h. Pretreatment with
SN-38 inhibited the
5 - FU - induced increase of
TS protein . -
pubmed
The synergistic effect induced by the combination of
SN-38 and
5 - FU may be attributable to the
SN-38 - induced suppression of
TS protein . -
pubmed
Furthermore ,
uracil and
5 - chloro-2,4 - hydroxypyridine , which are clinically available
dihydropyrimidine dehydrogenase inhibitors , enhanced
5 - FU - induced growth inhibition . -
pubmed
This may be the reason why Tk -
GK is not inhibited by
fructose 1,6 - bisphosphate , because the
fructose 1,6 - bisphosphate binding site is produced only when a tetrameric structure is formed . -
pubmed
Anticoagulant surface modification of
titanium via layer - by - layer assembly of
collagen and sulfated chitosan multilayers . -
pubmed
In the present work ,
collagen ( Col ) and sulfated chitosan ( SCS ) multilayers were coated on pure
titanium using a layer - by - layer ( LBL ) self - assembly technique . -
pubmed
Platelet adhesion in vitro , partial activated
thromboplastin time ( APTT ) and
prothrombin time ( PT ) assays were used to evaluate the hemocompatibility of the Col - SCS multilayer coated
titanium . -
pubmed
An agonist - antagonist interaction model for
prolactin release following
risperidone and
paliperidone treatment . -
pubmed
A competitive agonist - antagonist interaction model described the competition between these drugs and
dopamine for the
D ( 2 ) receptors that regulate
prolactin release . -
pubmed
Tolerance development was explained by a feedback loop with
prolactin stimulating
dopamine release , whereas models wherein tolerance is described in terms of depletion of a
prolactin pool did not explain the data well . -
pubmed
It is originally indicated that the key mediator of the cholinergic anti - inflammatory pathway ,
acetylcholine (
ACh ) , inhibits
cytokine release directly via the
alpha7 nicotinic ACh receptor (
nAChR ) expressed on macrophages . -
pubmed
OTX2 may play a role in development of the retinal
pigment epithelium and photoreceptor differentiation , whereas
OTX1 may be important in ciliary body development . -
pubmed
By in situ hybridization and immunohistochemistry , we show that
PAX6 was primarily localized to nuclei of the neural retina ,
OTX2 was localized to the nuclei of retinal
pigment epithelium and
OTX1 expression was confined to anterior retina . -
pubmed
Using cell lines and measurement procedures to isolate the effects on individual membrane transport proteins ,
crofelemer at 50 microM had little or no effect on the activity of epithelial
Na (+ ) or K (+ ) channels or on
cAMP or
calcium signaling . -
pubmed
Crofelemer inhibited the
cystic fibrosis transmembrane regulator (
CFTR )
Cl (- ) channel with maximum inhibition of approximately 60 % and an IC(50 ) approximately 7 microM. -
pubmed
Crofelemer action at an extracellular site on
CFTR produced voltage - independent block with stabilization of the channel closed state . -
pubmed
Crofelemer did not affect the potency of
glycine hydrazide or
thiazolidinone CFTR inhibitors . -
pubmed
Crofelemer action resisted washout , with & lt;50 % reversal of
CFTR inhibition after 4 h.
Crofelemer was also found to strongly inhibit the intestinal
calcium - activated
Cl (- ) channel
TMEM16A by a voltage - independent inhibition mechanism with maximum inhibition & gt;90 % and IC(50 ) approximately 6.5 microM. -
pubmed
In this paper , we investigate by Surface Plasmon Resonance (
SPR ) if
captavidin , a recently described
biotin - binding regenerable protein , could be used to bind and release biotinylated biocomponents for the development of regenerable biosensors . -
pubmed
Because
pyrazinoic acid is the active moiety , it should also increase overall
pyrazinamide activity and , because most resistance arises in the
pncA gene that converts
pyrazinamide to
pyrazinoic acid , it should act on most
pyrazinamide resistant
strains . -
pubmed
On multivariable analysis , maximum , average , or standard deviation of
glucose values predicted OS and maximum or average
glucose values predicted nonrelapse mortality (
NRM ) . -
pubmed
The
oxysterol 27 - hydroxycholesterol increases
beta - amyloid and oxidative stress in retinal pigment epithelial cells . -
pubmed
Levels of
Abeta peptide , mitochondrial and endoplasmic reticulum (
ER ) stress markers ,
Ca2 + homeostasis ,
glutathione depletion , reactive
oxygen species ( ROS ) generation ,
inflammation and cell
death were assessed using ELISA , Western blot , immunocytochemistry , and specific assays . -
pubmed
RESULTS :
27 - OHC dose - dependently increased
Abeta peptide production , increased levels of
ER stress specific markers
caspase 12 and
gadd153 ( also called
CHOP ) , reduced mitochondrial membrane potential , triggered
Ca2 + dyshomeostasis , increased levels of the
nuclear factor kappaB (
NFkappaB ) and
heme - oxygenase 1 (
HO-1 ) , two proteins activated by oxidative stress . -
pubmed
CONCLUSIONS : The
cholesterol metabolite
27 - OHC is toxic to
RPE cells . -
pubmed
The deleterious effects of this
oxysterol ranged from
Abeta accumulation to oxidative cell damage . -
pubmed
The
CP and the CMT markedly decrease in the presence of
NaCl due to the
dehydration of the
polyethylene oxide shell . -
pubmed
[
Granulocyte colony stimulating factor - producing
spindle cell renal cell carcinoma successfully treated by chemotherapy consisting of
gemcitabine and
doxorubicin ] . -
pubmed
Nafion coating the
ferrocenylalkanethiol and encapsulated
glucose oxidase electrode for amperometric
glucose detection . -
pubmed
This paper describes the fabrication and application of a complex electrode --
Nafion film coating
ferrocenylalkanethiol (
FcC ( 11 ) SH ) and encapsulated
glucose oxidase ( GOD ) on a gold electrode . -
pubmed
There were no significant
amino acid mutations of the
hemagglutinin (
HA ) and
neuraminidase (
NA ) sites of the viruses , including the receptor binding , glycosylation , antigenic and catalytic sites and
NA inhibitor targets . -
pubmed
All the viruses had polybasic
amino acids at the
HA cleavage site . -
pubmed
Platelet response to agonists (
TRAP ,
collagen ,
ADP ) and to hypotonic
shock decreased ,
CD62P expression increased in both storage media over time . -
pubmed
These data suggest that
HCQ , a common medication used to treat
RA , possesses beneficial effects upon
insulin sensitization . -
pubmed
This result was associated with increased
sFlt-1 ,
VEGF receptor-2 (
VEGFR-2 ) ,
VEGFR-3 and
endoglin levels but decreased
NO metabolites . -
pubmed
MgSO4 therapy ameliorated systolic
hypertension and
proteinuria and decreased
sFlt-1 ,
VEGFR-2 ,
VEGFR-3 and
endoglin levels but increased
NO metabolites in the treated group . -
pubmed
RESULTS :
ATO treatment induced up - regulation of some pro - apoptotic genes , such as
HRK ,
BAK1 ,
CASPASE-5 ,
BAD ,
TNFRSF1A , and
BCL2L14 and down - regulation of
ICEBERG . -
pubmed
CONCLUSIONS : These results represent the basis for a possible association of
ATO with other biological compounds able to modify the apoptotic pathways , such as inhibitors of the
BCL2 family . -
pubmed
The base substitutions change a
glycine to arginine in the
fibronectin type 3 domain of
nephrin and a
proline to arginine in a conserved
proline - rich region in
Neph3 . -
pubmed
Survival analysis to verify the impact of these gene polymorphisms on the clinical outcome of
lung cancer showed that
lung squamous cell carcinoma patients with the Thr /
Met genotype at
XRCC3 had a significantly shorter survival time than those with the Thr / Thr genotype ( 13 months versus 48 months ; log - rank test , p & lt ; 0.0001 ) . -
pubmed
PPi hydrolysis by soluble
inorganic pyrophosphatase (
iPPase ) has been considered as an important homeostatic mechanism . -
pubmed
Furthermore , our results revealed that
COE inhibited the transcriptional activity of
MMP-9 by suppression of the binding activity of
NF - kappaB in the MMP-9 promoter , and inhibited
IkappaBalpha phosphorylation and nuclear translocation of
NF - kappaB. -
pubmed
CONCLUSIONS :
COE inhibits
IL-1beta and
TNF - alpha combination - induced FLSs invasion by suppressing
NF - kappaB - mediated
MMP-9 expression . -
pubmed
Immunocytochemisty demonstrated
RPE - specific expression profiles in cells after 12 - weeks of co - culture on RWSF /
PCL / Gt membranes . -
pubmed
Methionine sulfoxide reductase (
MSR ) repairs oxidized
Met and protects organisms from oxidative damage . -
pubmed
We found that
ZmMSR genes were induced by
polyethylene glycol (
PEG ) and
NaCl , both known to generate oxidative stress . -
pubmed
( 99m ) Tc - MDP SPECT /
CT as the one - stop imaging modality for the diagnosis of early setting of
Kienbock ' s disease . -
pubmed
( 99m ) Tc - Methylene diphosphonate (
MDP ) triple phase bone scintigraphy (
BS ) has a role in early diagnosis of
Kienbock ' s disease , especially when the X - ray is negative . -
pubmed
Well - defined depth profiles of
delta ( 18 ) O ,
Al , and sea - salt -
Na (+ ) allowed the determination of chronology of the snow pit that spanned approximately 6 years from
spring 2003 to summer 2009 . -
pubmed
Se concentrations were at a low pg / g level , ranging from 7.2 to 45 pg / g , and exhibited high variability with generally higher values during winter and
spring and lower values during summer and fall . -
pubmed
High
Se / MSA ratios are generally observed in the winter and
spring snow layers , in which the
Se concentrations were relatively high ( & gt;20 pg / g ) . -
pubmed
This study tests the hypothesis that preservation of
GLP-1 by the
GLP-1 receptor agonist
liraglutide or the
dipeptidyl peptidase-4 (
DPP-4 ) inhibitor
linagliptin is associated with a reduction of
angiotensin ( Ang ) II - induced
cardiac fibrosis . -
pubmed
Liraglutide ( 0.3 mg / kg ) was subcutaneously injected twice daily or
linagliptin ( 8 mg / kg ) was administered via oral gavage daily during
Ang II infusion . -
pubmed
Liraglutide and
linagliptin comparatively reduced the protein level of the
Ang II AT1 receptor and up - regulated the
AT2 receptor as identified by a reduced
AT1 /
AT2 ratio ( 0.4 + - 0.02 and 0.7 + - 0.01 vs. 1.4 + - 0.2 in control , p & lt ; 0.05 ) , coincident with the less locally - expressed
AT1 receptor and enhanced
AT2 receptor in the myocardium and peri - coronary vessels . -
pubmed
Both drugs significantly reduced the populations of macrophages ( 16 + - 6 and 19 + - 7 vs. 61 + - 29 number / HPF in control , p & lt ; 0.05 ) and
alpha - SMA expressing myofibroblasts ( 17 + - 7 and 13 + - 4 vs. 66 + - 29 number / HPF in control , p & lt ; 0.05 ) , consistent with the reduction in expression of
TGFbeta1 and
phospho -
Smad2 / 3 , and up - regulation of
Smad7 . -
pubmed
Here , we show that
pHnuc dynamics can be imaged using the DNA - binding dye
Hoechst 33342 . -
pubmed
Cardiac myocyte
pHnuc changed in response to maneuvers that alter nuclear
Ca ( 2 + ) signals . -
pubmed
Blocking
Ca ( 2 + ) release from
inositol-1,4,5 - trisphosphate receptors stably alkalinized the nucleus . -
pubmed
The results also showed a weak positive correlation between HbA1c and
TSH ( r = 0.212 , P = 0.034 ) and insignificant correlation with
thyroxin T4 ( r = -0.018 , P = 0.855 ) . -
pubmed
There was a weak positive correlation between HbA1c and total
cholesterol and
low density lipoprotein ( r = 0.258 , P = 0.001 ) , ( r = 0.297 , P = 0.003 ) , respectively . -
pubmed
CONCLUSION : It is concluded that increased blood
glucose could trigger anterior pituitary gland to increase secretion of
TSH , whereas there was no direct correlation between increased glycemic index and the rate of
thyroxine secretion . -
pubmed
Key strategies included providing
alcohol - based handrub dispensers at points of care , designing educational programs tailored to the needs of different health care workers , placement of general and individual reminders in the workplace , and establishment of evaluation and feedback for
HH compliance and
infection rates . -
pubmed
A combination of X - ray diffraction , thermal analysis , FTIR , observations of isotropic chemical shifts of
( 1 ) H in distinct solvents , their temperature dependence and spin - lattice relaxation time constants (
T1 ) , solution ( 1D and 2D )
( 13 ) C nuclear magnetic resonance ( NMR ) , and solid - state
( 13 ) C NMR ( CPMAS NMR ) provides valuable structural information and structural elucidation of micronized
EFZ and
heptane - recrystallized polymorphs ( EFZ / HEPT ) . -
pubmed
Following 24h of incubation , cells were fixed and stained for migration and fluorescence analyses and the supernatant collected for quantification of
total protein ,
hydroxyproline ,
bFGF ,
IL-6 and
IL-10 . -
pubmed
Synthesis and Biological Evaluation of
1 - Methyl-1H - indole - Pyrazoline Hybrids as Potential
Tubulin Polymerization Inhibitors . -
pubmed
A series of
1 - methyl-1H - indole - pyrazoline hybrids were designed , synthesized , and biologically evaluated as potential
tubulin polymerization inhibitors . -
pubmed
Cryogenic transmission electron microscopy ( Cryo -
TEM ) images showed a substantial number of liposomes in addition to cubosomes in the dispersion containing 4 - 1 ( w / w )
phytantriol - to - F127 prepared by the BU approach compared to very low liposome content with the TD approach . -
pubmed
In control group the
HK ,
PFK and
ALD activities showed strong positive correlation with blood
sugar level while
PGI activity did not show any correlation . -
pubmed
The
LDH activity only in the control group showed positive correlation with marginal increase with increasing concentrations of
glucose . -
pubmed
The effect of
1,25 - dihydroxyvitamin D3 on
TSLP ,
IL-33 and
IL-25 expression in respiratory epithelium . -
pubmed
1,25D3 increased
TSLP concentration in unstimulated nasal epithelial cells , but did not influence
IL-33 and
IL-25 expression . -
pubmed
Simultaneously
1,25D3 augmented
IL-25 production in the same model of stimulation . -
pubmed
We also found that physical activity levels negatively affect BMI ( -0.148 ) and positively affect
high - density lipoprotein (
HDL ) ( 0.106 ) ; sociodemographic background negatively affects diet ( -0.522 ) and
BMI ( -0.352 ) ;
HDL positively affects total
cholesterol ( 0.230 ) and has a negative effect on
BMI ( -0.108 ) ,
triglycerides ( -0.128 ) and waist circumference ( -0.104 ) . -
pubmed
BMI and
HDL ( -0.002 ) together mediate the effect of physical activity on
diabetes status and similarly
HDL and
tryglycerides ( -0.005 ) also mediate the effect of physical activity on
diabetes status . -
pubmed
ARII exhibits a photocycle including a quasi - equilibrium state of M , N , and
O ( M N O ) at near neutral and above pH values . -
pubmed
These photoproducts , termed
O1 and
O2 , may be even produced at physiological pH , although they are not clearly observed under this condition due to the existence of a long M - N - O equilibrium . -
pubmed
Effects of
Triflusal and
Clopidogrel on the Secondary Prevention of
Stroke Based on
Cytochrome P450 2C19 Genotyping . -
pubmed
Moreover , we directly demonstrated that nano -
ZnO was adsorbed on the algal B - EPS and entered into the algal cells by transmission electron microscope coupled with energy dispersive X - ray (
TEM - EDX ) . -
pubmed
Rolipram , a specific
phosphodiesterase-4 inhibitor which is traditionally used as an anti - depressant drug , has been recently proven to exert neuroprotective effects in several central nervous system insults . -
pubmed
Rolipram treatment remarkably promoted the expression of
Sirtuin 1 (
SIRT1 ) while inhibited
NF - kappaB activation . -
pubmed
Moreover ,
rolipram significantly inhibited the activation of microglia as well as down - regulated the expression of pro - inflammatory
cytokines TNF - alpha ,
IL-1ss , and
IL-6 . -
pubmed
In addition ,
rolipram increased the expression of protective
cytokine IL-10 . -
pubmed
The neuroprotective effects of
rolipram were associated with regulating the
SIRT1 /
NF - kappaB pathway . -
pubmed
Alcohol dehydrogenase (
ADH ) is the principal enzyme responsible for the metabolism of
ethanol . -
pubmed
The quantitative contributions of
ADH isozymes and ethnically distinct allozymes to cellular
ethanol metabolism remain poorly understood . -
pubmed
The organ simulations indicate that in homozygous
ADH1B * 1 /* 1 livers , a representative genotype among ethnically distinct populations due to high prevalence of the allele , major contributors at 1 to 10 mM
ethanol are
ADH1B1 ( 45 % to 24 % ) and the
ADH1C allozymes ( 54 % to 40 % ) . -
pubmed
The model simulations suggest that slightly higher or similar
ethanol elimination rates for
ADH1B * 2 /* 2 and
ADH1B * 3 /* 3 individuals compared with those for
ADH1B * 1 /* 1 individuals may result from higher hepatocellular
acetaldehyde . -
pubmed
FcalphaRI binding at the
IgA1 CH2 - CH3 interface induces long - range conformational changes that are transmitted to the hinge region . -
pubmed
FcalphaRI binds to the
IgA1 - Fc domain (
Fcalpha ) at the
CH2 - CH3 junction and , except for CH2 L257 and L258 , all side - chain contacts are contributed by the CH3 domain . -
pubmed
The energetic contribution of each
IgA residue in the binding interface was assessed by
alanine - scanning mutagenesis and equilibrium surface plasmon resonance (
SPR ) . -
pubmed
Surprisingly , individual mutation of
CH2 residues L257 and L258 , found at the periphery of the
FcalphaRI binding site , dramatically reduced binding affinity . -
pubmed
Comparison of antibody : receptor complexes involving
IgA or its precursor
IgY revealed a conserved receptor binding site at the
CH2 - CH3 junction ( or its equivalent ) . -
pubmed
Given the importance of residues near the
CH2 - CH3 junction , we used coarse - grained Langevin dynamics simulations to understand the functional dynamics in
Fcalpha . -
pubmed
Subsequent
SPR experiments confirmed that
FcalphaRI binding to the
Fcalpha CH2 - CH3 junction altered the kinetics of
HAA lectin binding at the
IgA1 hinge . -
pubmed
Compared to
ApKir2 ,
ApKir1 mediated currents that were larger in magnitude , more sensitive to
barium , and less inhibited by small molecule
VU041 . -
pubmed
Shogaol but not
gingerol has a neuroprotective effect on
hemorrhagic brain injury : Contribution of the
alpha , beta - unsaturated carbonyl to
heme oxygenase-1 expression . -
pubmed
We investigated the effects of
shogaol , which has an
alpha , beta - unsaturated carbonyl group , and
gingerol , which does not , on primary - cultured microglia to understand how the
alpha , beta - unsaturated carbonyl interacts with
Kelch - like ECH - associated protein ( Keap ) 1 . -
pubmed
Shogaol ( 1 muM ) but not the same concentration of
gingerol significantly increased
heme oxygenase ( HO )- 1 protein levels in cultured microglia without
cytotoxicity . -
pubmed
In addition ,
shogaol suppressed the release of the
inflammation marker
nitric oxide induced by 30 U / ml
thrombin treatment . -
pubmed
A docking simulation suggested that the
alpha , beta - unsaturated carbonyl of shogaol but not
gingerol interacts with
Keap1 . -
pubmed
The transcription inhibitor
actinomycin D ( 0.1 mug / ml ) markedly blocked the increase of
HO-1 mRNA levels by
shogaol . -
pubmed
Intracerebroventricular injection of 0.2 nmol
shogaol increased striatal
HO-1 protein levels and rescued
ICH - induced
neuron loss . -
pubmed
Thus , the
alpha , beta - unsaturated carbonyl is necessary for the interaction of compounds , such as
shogaol , with
Keap1 , and these findings may be useful for screening novel
ICH therapeutic agents that increase
HO-1 expression . -
pubmed
Nicotine functions as a discriminative stimulus in modulating operant behavior as well as Pavlovian feature stimuli in modulating the conditional responding (
CR ) to exteroceptive
CS US contingencies . -
pubmed
This experiment evaluated the interaction among Pavlovian feature positive (
FP ) and feature negative ( FN ) effects of
nicotine on concurrently occurring operant SD and SDelta effects . -
pubmed
For the other group ( n = 8)
nicotine served as the SDelta ( lever pressing non - reinforced ) and
FP for the
CS , with saline serving in the reverse roles . -
pubmed
Consecutive brief non - reinforcement tests revealed that : A ) rates of lever pressing were significantly greater in SD than SDelta with
nicotine and saline suggesting strong operant discriminative stimulus control ; B )
FP responding to the light
CS with
nicotine and saline was evident ; and C ) FN suppression of the
CR with
nicotine was not evident but weak under saline . -
pubmed
The heteroblastic switch optimizes
carbon efficiency in the juvenile phase ( by sterile shoots ) in the mesic
post - fire conditions . -
pubmed
Blood
glucose ,
insulin levels , lipid profiles , and oxidative stress markers were measured . -
pubmed
Ca and dairy product intakes may be inversely associated with all - cause and cause - specific mortality , and non -
Ca components of dairy products , such as
insulin - like growth factor-1 , may be independently associated with mortality . -
pubmed
The MM - PB(GB)SA ( molecular mechanics - Poisson Boltzmann / Generalized Born ) Surface Area ) binding free energies calculated using the Amber force fields bsc0 and OL15 correlate well with the NMR titration and binding affinity measurements , with both calculations correctly identifying the
EPI as the strongest binder to the hybrid-2 telomeric
G4 wtTel26 . -
pubmed
We have also performed binding free energy calculation using the more rigorous double decoupling method ( DDM ) ; however , the results correlate less well with the experimental trend , likely due to the difficulty of adequately sampling the very large conformational reorganization in the
G4 induced by the
protoberberine binding . -
pubmed
Here , we investigated the occurrence of 21
organochlorine pesticides ( OCPs ) , 14 current use pesticides ( CUPs ) , 47 halogenated flame retardants ( HFRs ) , and 19
organophosphate esters (
OPEs ) in air around Las Cruces ( LC ) and La Selva ( LS ) Biological Stations , Costa Rica , and Kibale National Park ( KNP ) , Uganda using passive air samplers ( PAS ) with
polyurethane foam (
PUF ) discs ( PAS -
PUF ) . -
pubmed
In this paper , astrocytic
calcium oscillations and neuronal firing dynamics are presented by De Pitta and
IF ( Integrated & amp ; Fire ) models , respectively . -
pubmed
This low - cost hardware architecture for the astrocyte model is able to show the essential features of different types of
Ca2 + modulation such as amplitude modulation (
AM ) , frequency modulation ( FM ) , or both modes (
AFM ) . -
pubmed
This new neuromorphic circuit of astrocyte is able to successfully demonstrate
AM / FM /
AFM calcium signaling in its real operation on FPGA and has applications in self - repairing systems . -
pubmed
AxyA hydrolyzes
isoprimeverose (
alpha - D - xylopyranosyl -( 1 6 )- D - glucopyranose ) into
D - xylose and
D - glucose and shows hydrolytic activity with other xyloglucan
oligosaccharides such as
XXXG (
heptasaccharide ,
Glc4Xyl3 ) and
XLLG (
nonasaccharide ,
Glc4Xyl3Gal2 ) . -
pubmed
The intracellular localization of
AxyA may help determine the order of
hydrolases acting on xyloglucan
oligosaccharides . -
pubmed
Fingolimod and
Dimethyl - Fumarate - Derived
Lymphopenia is not Associated with Short - Term Treatment Response and Risk of Infections in a Real - Life
MS Population . -
pubmed
Mean
glucose values ,
insulin values given for correction per hospitalization , and the number of
hypoglycemic events (
glucose < 70 mg / dL ) were documented . -
pubmed
No significant differences were found regarding
glucose level ( P = 0.462 ) and administered
insulin units ( P = 0.639 ) . -
pubmed
In addition ,
trastuzumab ( 10 muM ) induced SAN - PV conduction block and burst firings , which were blocked by
wortmannin ( a
PI3 K inhibitor , 100 nM ) . -
pubmed
Settings and Design : Matched paired comparison between the TACE -
apatinib and TACE alone group using 1:2
PSM was utilized . -
pubmed
Towards integration of 64Cu - DOTA -
trastuzumab PET -
CT and MRI with mathematical modeling to predict response to neoadjuvant therapy in
HER2 + breast cancer . -
pubmed
After randomization , the first group ( mask then BVGA , n = 12 ) began breathing room air through the
face mask , followed by 100 %
O2 . -
pubmed
By contrast , while breathing through a
face mask , there were 8 of 25 ( 32 % ) and 5 of 25 ( 20 % ) cases of suspected leak in air and 100 %
O2 , respectively ( air : p = 0.002 ; 100 % O2 : p = 0.014 by McNemar test ) . -
pubmed
The standard
molybdenum blue method , APHA 4500
PE has a detection limit of 30 mugL-1
phosphate ( 10 mugL-1
phosphorus ) in freshwater with a 5 cm cuvette . -
pubmed
The second - most - soluble material ,
NiO D , and the third ,
NiO A , caused evident
pulmonary inflammation , and the responses persisted for at least 91 days with
collagen proliferation . -
pubmed
A useful intravital sign of the formation of a
postinfarction scar is provided by the examination of
collagen metabolites --
oxyproline bound with
collagen - like plasma protein and free serum
oxyproline and urine excretion of total
oxyproline . -
pubmed
A sensitive , fluorometric method for the assay of
microsomal hydroxylase ; N - demethylation of
p - chloro - N - methylaniline . -
pubmed
A peptide corresponding to the 11
amino acid residues of the NH2 - terminal portion in the sequence of
carcinoembryonic antigen(
CNTHETIC CEA ( 1 - 11 ) peptide was attached by means of a water - soluble
carbodiimide reagent to multichain
poly ( DL - alanine ( as well as to
bovine serum albumin . -
pubmed
3
Gastrin release and acid secretion produced by
litorin was more rapid in onset but less intense and less sustained than that elicited by
bombesin . -
pubmed
4 Gall bladder contraction stimulated by
litorin was probably caused by a double action of the peptide , directly on the bladder smooth muscle , and indirectly by
cholecystokinin release . -
pubmed
The immunosuppressive protocol included
thymoglobulin during the induction period and a classical 3 - drug therapy , with a rapid tapering off of
prednisone over 3 weeks . -
pubmed
The rate of consumption of
oxygen was 1.2 + /- 0.1 mmol / min , the respiratory quotient was close to unity , and close to half of the
PDH was in its active form ; therefore , virtually all
ATP synthesis should require flux through
PDH . -
pubmed
Both
ACTH and
cortisol exhibit complex secretory patterns demonstrating ultradian and circadian rhythms . -
pubmed
Immunoreactive
ACTH and
cortisol were measured in all samples and
glucose in selected samples . -
pubmed
Plasma
glucose was lower than
preinsulin levels at 10 min , declined rapidly between 10 and 30 min , and remained low 30 - 60 min after
insulin injection in both treatment groups . -
pubmed
The increase in mean plasma hormone levels during
hypoglycemia was predominantly due to an increase in amplitude of secretory
pulses for
ACTH and
cortisol compared with the 30 min before
insulin . -
pubmed
Dexamethasone significantly lowered mean ACTH and
cortisol levels and prevented alteration in plasma
ACTH and
cortisol secretion during
hypoglycemia but did not totally ablate pulsatile activity of either hormone . -
pubmed
The amplitude of
ACTH and
cortisol pulses was significantly decreased by
dexamethasone treatment . -
pubmed
The frequency of
cortisol but not
ACTH pulses was also significantly decreased. -
pubmed
However , reduction of
interleukin-2 (
IL-2 ) production and mitogen responses induced by GvHR were not rescued by
prodigiosin 25 - C treatment . -
pubmed
Prodigiosin 25 - C moderately prolonged survival of major histocompatibility (
MHC)-mismatched skin grafts . -
pubmed
Nine different tissues were extracted with an
acid acetate - buffer ( lung , heart , brain , adrenal , aorta , skeletal muscle , spleen and liver ) or with
KSCN - buffer ( kidney ) , and
antiplasmin activity was evaluated by a spectrophotometric method . -
pubmed
The results indicate that an appropriate sympathetic neural environment is needed for
cyclosporine to have an effect on
ornithine decarboxylase activity in lymphoid tissue . -
pubmed
The
amino acid sequence deduced from the exons matches perfectly the one previously determined from the
T - cell cyclophilin cDNA . -
pubmed
The assignment of compartmental designation to the
T2 component remains a problem except for homogeneous structures easily identifiable anatomically ( ventricles , superior sagittal
sinus , globe of the eye ) . -
pubmed
The predominance of the interstitial and plasma compartment , the invisibility of part of the intracellular
sodium , and the difficulty in imaging the very fast
T2 component of visible intracellular
sodium make the usual
Na spin - echo image essentially an image of the interstitial and plasma space . -
pubmed
Unlike conventional
barium studies
CT is able to demonstrate completely the
intussusception complex . -
pubmed
Tissue patterns of gene expression were analyzed by measuring mRNA levels and incorporation of radioactive
amino acids for
cystatin C and
beta 2 - microglobulin , the two extracellular proteins in the brain with the highest ratio of concentration in cerebrospinal fluid over that in blood plasma . -
pubmed
The primary structure of
rat cystatin C mRNA from choroid plexus was determined by
nucleotide sequencing of cloned cDNA and the tissue patterns of gene expression were analysed by RNA blot analysis and in situ hybridization . -
pubmed
Cystatin C was found to be composed of 120
amino acids and to contain a potential site for N - linked glycosylation . -
pubmed
Choroid plexus pieces in culture secreted radioactive
cystatin C when incubated with radioactive
leucine . -
pubmed
Changes in organ and glandular weights and
serum thyrotropin levels reflected changes in serum
thyroid hormone concentrations . -
pubmed
In addition , the
zinc - deficient state was found to be protective against
thiouracil - induced suppression of the
microsomal - monooxygenase and
thyroxine-5 '- monodeiodinase enzyme complex . -
pubmed
Effect of
cimetidine on
enzyme actuation .... -
pubmed
The highly water - soluble , quaternarized derivatives of
ellipticine have no bactericidal effect and do not induce the synthesis of
rec A protein . -
pubmed
Groups of four
ganglia were incubated for 4 h with 20 nM [ 125I]
alpha - bungarotoxin alone or with radioactive
alpha - bungarotoxin plus either 1 microM unlabeled
alpha - bungarotoxin or 100 microM
d - tubocurarine . -
pubmed
The
EPR spectra of the
SH - groups spin - labeled with
maleimide or
iodoacetamide show the existence of two populations of
sulfhydryl groups , differing in their mobility ( one weakly , the other strongly immobilized ) . -
pubmed
The
phospholipase activity , subordinate to
hypoxia , decreased by 1/5 compared to its value without treatment and the phospholipid composition of the broncho - alveolar lung lavage remained unchanged whereas after
hypoxia without treatment the
phosphatidylcholines level decreases by 26 % . -
pubmed
We also showed that this effect could not be due to the only action of reduced
sulphur : different concentrations of
sulphur solutions had no action on the
phospholipase A activity subordinate to
hypoxia . -
pubmed
This effect was demonstrable only in the presence of
insulin and
dexamethasone in cultural media . -
pubmed
However , the use of
monoclonal anti - Thy 1.2 antibodies failed to prove that
antigen in question was
structural virion protein or
Thy 1.2 antigen . -
pubmed
Effects of
5 - halopyrimidinones with antiviral and antineoplastic activity on
murine cytochrome P-450 . -
pubmed
These results suggest that
5 - halopyrimidinones can depress
cytochrome P-450 levels and that this
depression may affect the metabolism of other drugs by
cytochrome P-450 . -
pubmed
Sodium dodecyl sulfate -
polyacrylamide gel electrophoresis analysis of the
Sarkosyl - resistant proteins revealed a composition similar to that of the proteins exposed on the surfaces of viable cells , as determined by the
lactoperoxidase-125I radioiodination method . -
pubmed
In vitro , the
diphosphate of
azidocytidine was earlier shown to inhibit
mammalian ribonucleotide reductase , while the
triphosphate did not inhibit DNA replication in isolated cell nuclei . -
pubmed
In intact cells , however ,
deoxyribonucleoside triphosphate pools were not decreased by
azidocytidine in a manner expected from an inhibitor of
ribonucleotide reductase . -
pubmed
Azidocytidine was transformed to an active metabolite by phosphorylation with
deoxycytidine kinase . -
pubmed
By days 15 - -16 ,
dieldrin had reduced the
pentobarbital sleep time by 54 % ( P & lt ; 0.05 ) , increased the liver mass by 8 % ( P & lt ; 0.05 ) , and increased hepatic microsomal
cytochrome P-450 by 66 % ( P & lt ; 0.05 ) . -
pubmed
Orally active and long - term acting
insulin - mimetic
vanadyl complex :
bis ( picolinato ) oxovanadium ( IV ) . -
pubmed
It is well known that excess amounts of
antigen modulate
TCR / CD3 complex on antigen - specific T lymphocytes . -
pubmed
Single - base substitutions in the immediate 5'-flanking region of the fetal G gamma and A gamma
globin genes have been associated with non - deletional forms of hereditary persistence of
fetal haemoglobin (
HPFH ) . -
pubmed
Previously , the sole promoter mutation associated with
HPFH in British individuals has been the T to C substitution at position -198 relative to the A gamma
globin gene
CAP site . -
pubmed
The mutation was first detected by examining the amplified 5 ' regions of both the
G gamma and A gamma
globin genes for heteroduplex formation after electrophoresis in a Hydrolink gel . -
pubmed
The final step in the synthesis of
PtdIns ( 4,5 ) P2 is the phosphorylation of
PtdIns ( 4 ) P- by
PtdIns ( 4 ) P 5 - kinase (
PIP5 K ) . -
pubmed
STE4sd mutations resulted in
amino acid substitutions in the N - terminal region of
Ste4p , proximal to the first of seven repeat units conserved in
G protein beta subunits . -
pubmed
Wild - type cells that overexpressed
STE4sd alleles displayed apparently normal initial responses to
pheromone as judged by quantitative mating ,
G1 arrest and transcriptional assays . -
pubmed
The entry criteria were serum total
cholesterol ( TCHOL ) between 6.2 and 7.8 mM and
low - density lipoprotein (
LDL )
cholesterol between 4.3 and 50 mM on a standard diet and after the 2 - week run - in period of placebo treatment . -
pubmed
There was a significant linear dose response with regard to the decrease in
LDL cholesterol , TCHOL , and
triglycerides (
TG ) and the increase in
high - density lipoprotein (
HDL )
cholesterol after 8 weeks of therapy . -
pubmed
At each dose level ,
simvastatin reduced TCHOL and
LDL cholesterol . -
pubmed
Doses of
simvastatin & gt ; or = 5 mg moderately increased
HDL cholesterol and reduced serum
TG . -
pubmed
Substrate cycling from
phosphoenolpyruvate to
pyruvate [ via
pyruvate kinase (
PK ) ] and from
oxaloacetate to
pyruvate [ via
malic enzyme ( ME ) ] relative to the
pyruvate carboxylase ( PC ) flux [ i.e. -
pubmed
MARCKS specifically binds to
phosphatidylserine but not
phosphatidylcholine . -
pubmed
The binding of
MARCKS to
phosphatidylserine was abolished on
protein kinase C - dependent phosphorylation . -
pubmed
These data suggest that
phosphatidylserine is a membranous target molecule of
MARCKS . -
pubmed
Phosphoramidon and the
neutral endopeptidase (
NEP ) inhibitors
thiorphan and
candoxatrilat blocked the vasoconstriction caused by big
ET-1 in endothelium - denuded RSA . -
pubmed
We have determined that the DNA sequence downstream of the well - characterized gonococcal
fbp gene contains two open reading frames : one designated
fbpB , which encodes a protein proposed to function as a cytoplasmic permease , and one designated
fbpC , which encodes a protein proposed to function as a
nucleotide - binding protein . -
pubmed
Four isolates , previously shown to be unrelated to each other by pulsed - field gel electrophoresis analysis , were positive for both genes by PCR , although
beta - lactamase production was not induced with
methicillin . -
pubmed
A
methionine at
amino acid position 11 of the
HLA class II DP alpha 1 chain correlates with the occurring disease after
infection ( relative risk 3.3 ) . -
pubmed
The lack of
adhesion molecule expression on CNS endothelium
molecules may be an explanation for the poor leukocyte recruitment during
Wallerian degeneration in CNS when compared with PNS . -
pubmed
campestris in deduced
amino acid sequence ) suggests that
SLG diversification predates speciation . -
pubmed
TPO induced
tyrosine phosphorylation of platelet proteins was completely absent ( 3 of 4 ) or markedly decreased ( 1 of 4 ) . -
pubmed
The catalytic effect of natural and non - natural
amino acid substitutions at the
P1 position in a 5A/5B peptide substrate was investigated . -
pubmed
NS3 -
NS4A demonstrated a marked catalytic preference for the
cysteine residue commonly found in authentic substrates . -
pubmed
A second class of inhibitors is
NS4A - dependent and contains a natural or non - natural cyclic
amino acid substituted for the commonly found
P1 ' residue
serine . -
pubmed
Here we show that
cholesterol feeding also reduces the levels of the nuclear NH2 - terminal domains of
sterol regulatory element binding proteins (
SREBPs ) , which activate transcription of
sterol - regulated genes . -
pubmed
Cholesterol also appeared to reduce the proteolytic processing of
SREBP-2 . -
pubmed
In addition , at high levels of dietary
cholesterol the mRNA encoding
SREBP-2 declined and the amount of the precursor also fell , suggesting that
cholesterol accumulation also may inhibit transcription of the
SREBP-2 gene . -
pubmed
The high -
cholesterol diets reduced the amount of
low density lipoprotein receptor mRNA by 30 % and produced a more profound 70 - 90 % reduction in mRNAs encoding
3 - hydroxy-3 - methylglutaryl CoA synthase and reductase . -
pubmed
Treatment with
lovastatin and
Colestipol , which increases hepatic demands for
cholesterol , increased the amount of
SREBP-2 mRNA as well as the precursor and nuclear forms of the protein . -
pubmed
The percentage of large B cells from the male BXSB that expressed
CD40L was increased to 43 % by stimulation with
LPS . -
pubmed
However , resistance to commonly used antibiotics was very high , 94 % isolates were resistant to
ampicillin which corresponds to the
betalactamase production . -
pubmed
Intravenous administration of
H3 - receptor agonists significantly decreased
noradrenaline release by the heart ( R - HA at 2 micromol kg(-1 ) h(-1 ): +77 + /- 25 vs +405 + /- 82 ; BP 2.94 at 1 mg kg(-1 ): +12 + /- 11 vs +330 + /- 100 pg ml(-1 ) in control conditions , P & lt ; or = 0.05 ) , and increases in heart rate ( R - HA at 2 micromol kg(-1 ) h(-1 ): +26 + /- 8 vs +65 + /- 10 and BP 2.94 at 1 mg kg(-1 ): +30 + /- 8 vs 75 + /- 6 beats min(-1 ) , in control conditions
P & lt ; or = 0.05 ) , left ventricular pressure , and contractility . -
pubmed
Pyridostigmine which is widely used as pretreatment of
organophosphate poisoning protects
cholinesterases of peripheral nervous system . -
pubmed
Binding of
lectin appeared strongest on the adluminal membrane of these cell populations suggesting a role for the
carbohydrate moieties in nutrient transfer . -
pubmed
Protease inhibitors , inhibitors of the endothelial cell proliferation , suppressors of angiogenic growth factors ,
copper chelators , and other compounds interfering with the process of angiogenesis were screened for inhibition of
tumor angiogenesis and some of them are in clinical trials . -
pubmed
PURPOSE : We review our technique of ureteroscopic management of lower
pole renal calculi with
Nitinol basket displacement and
holmium laser stone fragmentation . -
pubmed
RESULTS : The
Nitinol device can be passed into the lower
pole through the fully deflected ureteroscope without any loss of deflection . -
pubmed
We also examined cells expressing this variant for sensitivity to the interstrand cross - linking agent ,
mitomycin C (
MMC ) , as
HDR mutant cell lines , including the
XRCC3 mutant , have been found to be hypersensitive to this DNA damaging agent . -
pubmed
Exposure of erythrocytes to
methylene blue shows the active role of
catalase in removing
hydrogen peroxide . -
pubmed
Methylene blue (
MB ) is a powerful reducing agent that is widely used in clinical practice as well as for metabolic studies of the erythrocyte . -
pubmed
In the presence of MB ,
catalase underwent inactivation even with the co - existence of active generation of
NADPH , leaving the
glutathione concentration unaffected . -
pubmed
The data obtained in the present investigation show , using a different tool (
MB ) , that
catalase is the active enzyme in
H2O2 detoxification and that its integrity is largely dependent on an adequate generation of
NADPH . -
pubmed
Stimulation with
testosterone of cells grown in depleted medium or exposed to
tamoxifen , resulted in the aggregation of the expanded
androgen receptor into characteristic cytoplasmic inclusions . -
pubmed
Differential modulation of
polyglutamine - expanded
androgen receptor aggregation by ligands in neuronal cells may partly explain the absence of an overt disease phenotype in female carriers of the
SBMA mutation and define conditions to explore further the role of
androgen receptor aggregation in the pathogenesis of
SBMA . -
pubmed
As a result , antibody titers detected by
PA were in parallel to the titers which detected by Nt test , and it was concluded that the differences in antibody titers would arise from the differences of test principles and viral
antigens . -
pubmed
Data obtained with the fpRFDR -
CT , DQCSA , and 2D MAS exchange solid - state NMR techniques , which provide independent constraints on the phi and psi backbone torsion angles between the labeled
carbonyl sites , indicate non - beta - strand conformations at G25 ,
S26 , and G29 . -
pubmed
Cortisol treatment had no apparent effect on placental mRNA expression for the
glucose transporters ,
GLUT-1 and
GLUT-3 . -
pubmed
Active peptides also activated
protein kinase B and
extracellular signal - regulated kinase (
ERK ) in vivo in a
wortmannin - sensitive manner while reducing recoverable cellular
p85 levels . -
pubmed
Prolactin releasing peptides modulate background firing rate and milk - ejection related burst of
oxytocin cells in the supraoptic nucleus . -
pubmed
Existence of
prolactin releasing peptide - immunoreactive cells in the dorsomedial nucleus and synaptic contact of
prolactin releasing peptide - immunoreactive terminals with
oxytocin cells was reported . -
pubmed
Experiments were done to test the effect of
prolactin releasing peptide on the electrical activity of
oxytocin cells in the supraoptic nucleus . -
pubmed
These results suggest that
prolactin releasing peptide may modulate electrical activity of
oxytocin cells not through its direct action on
oxytocin cells but through its action on area other than supraoptic nucleus . -
pubmed
It is conceivable that Tg polymorphisms , combined with the explosive mix of
iodine ,
TPO and
H2O2 necessary for
thyroid hormone synthesis , inadvertently provide the trigger for the
autoimmune thyroid response . -
pubmed
The inhibition of
caspases that target the
amino acid sequence DXXD abrogated
calnexin cleavage in apoptotic stimulus - treated cells . -
pubmed
The obstacle facing
Exo + polymerases for single
nucleotide polymorphism ( SNP ) detection could be bypassed by using primer-3'-termini modification . -
pubmed
ADP - ribosyl cyclase couples to
cyclic AMP signaling in the cardiomyocytes . -
pubmed
ADP - ribosyl cyclase (
ADPR - cyclase ) produces a
Ca ( 2 +)-mobilizing second messenger
cyclic ADP - ribose (
cADPR ) from
beta - NAD (+ ) . -
pubmed
In this study , we examined the molecular basis of which
beta - adrenergic receptor (
betaAR ) stimulation induces
cADPR formation and characterized cardiac
ADPR - cyclase . -
pubmed
Moreover , incubation of ventricular lysates with
isoproterenol ,
forskolin or
cAMP resulted in activation of
ADPR - cyclase that was inhibited by pretreatment with
H89 . -
pubmed
Characterization of partially purified cardiac
ADPR - cyclase showed a molecular mass of approximately 42 kDa and no cross - activity with
CD38 antibodies , and the enzyme activity was inhibited by
Zn ( 2 + ) but not
dithiothreitol . -
pubmed
Istradefylline , a novel
adenosine A2A receptor antagonist , for the treatment of
Parkinson ' s disease . -
pubmed
1 - Substituted pyrazolo [ 1,5 - c ] quinazolines as novel
Gly / NMDA receptor antagonists : synthesis , biological evaluation , and molecular modeling study . -
pubmed
A new set of
5,6 - dihydro - pyrazolo [ 1,5 - c ] quinazoline-2 - carboxylates ( 2 - 18 ) , bearing different substituents (
COOEt ,
Cl ,
Br ,
CH ( 3 ) , and
COOH ) at position-1 , were synthesized in order to investigate the influence of various groups at this specific position on
Gly / NMDA receptor affinity and/or selectivity . -
pubmed
The results obtained in this study have highlighted that a C-1 lipophilic substituent on the
pyrazolo [ 1,5 - c ] quinazoline-2 - carboxylate core shifts selectivity toward the
Gly / NMDA receptor , while a C-1 anionic
carboxylate residue is able to increase affinity toward this receptor subtype . -
pubmed
Furthermore , the
1,2 - dicarboxylic acids 13 and 14 are endowed with the highest
Gly / NMDA receptor binding activity ( K(i)=0.09 and 0.059muM , respectively ) , among the
pyrazoloquinazoline series of derivatives . -
pubmed
Administration of
secretin for
autism alters
dopamine metabolism in the central nervous system . -
pubmed
In addition , we investigated the association between improvement in symptoms and changes in the cerebrospinal fluid (
CSF )
homovanillic acid (
HVA),
5 - hydroxyindole-3 - acetic acid (
5 - HIAA ) , and
6R-5,6,7,8 - tetrahydro - L - biopterin (
BH ( 4 ) ) levels after administration . -
pubmed
These findings suggest that
secretin activated metabolic turnover of
dopamine in the central nervous system via BH(4 ) , improving symptoms . -
pubmed
As the conditions of successful use of the NLS properties to enhance gene transfer are not clear , and with the aim of detecting and defining the requirements of
NLS - enhanced transfection , we investigated gene delivery and expression into various cell lines with various DNA complexes ( lipoplexes or polyplexes ) that were formulated for various N / P ratios from various preformed
Acr - spacer - NLS / DNA complexes ( 1:1 , 5:1 and 10:1 molar ratio ) . -
pubmed
The peripherally restricted
mu - opioid receptor (
MOR ) antagonist
naloxone methiodide dose - dependently attenuated the effects of systemic
morphine . -
pubmed
BACKGROUND :
CYP2C9 is one of the major drug metabolizing enzymes for many drugs including
warfarin , NSAIDs and
losartan . -
pubmed
Diethylstilbestrol inhibits the
proton pump and the passive
H (+ ) conductance with about the same time course , at concentrations that have no effect on cytoplasmic streaming . -
pubmed
The vitrification solution contained 40 %
ethylene glycol , 18 %
Ficoll , and 0.3 M
sucrose in
PBS . -
pubmed
The embryos were placed for 1 to 2 min in vitrification
solution ( Group 1 ) or following exposure to 20 %
ethylene glycol in
PBS for 10 to 20 min ( Groups 2 and 3 ) . -
pubmed
Straws were warmed in
water ( 20 degrees C , 20 sec ) , and the contents were expelled with 0.5 M
sucrose in
PBS . -
pubmed
The activity of
milin is inhibited by
serine proteases inhibitors like
PMSF , APMSF and
DFP , but not by any other
protease inhibitors such as
E-64 and
PCMB . -
pubmed
The
enzyme is a
glycoprotein with detectable
carbohydrate moiety ( 7 - 8 % ) in its constitution , which is essential for the activity . -
pubmed
The numbers of
tryptophan ,
tyrosine and
cysteine residues in the sequence of
milin were estimated chemically and are 23 , 14 and 14 , respectively . -
pubmed
ChREBP binding to
fatty acid synthase and
L - type pyruvate kinase genes is stimulated by
glucose in pancreatic beta - cells . -
pubmed
The relative importance of the transcription factors
carbohydrate - responsive element binding protein (
ChREBP ) ,
sterol - responsive element binding protein-1c (
SREBP-1c ) , and
upstream stimulatory factor (
USF ) in the induction of lipogenic genes by
glucose remains unclear . -
pubmed
Both
ChREBP and
SREBP-1c were required for the induction of the
fatty acid synthase (
FAS ) promoter by
glucose , and chromatin immunoprecipitation ( ChIP ) assay revealed that
glucose induced the binding of both
ChREBP and
SREBP-1c to the
FAS promoter without affecting
USF2 binding . -
pubmed
By contrast , ChIP assay revealed that high
glucose prompted direct binding of
ChREBP , but not
SREBP-1c or
USF2 , to the
liver - type pyruvate kinase (
L - PK ) promoter . -
pubmed
This event was indispensable for the induction of the
L - PK gene by
glucose , as demonstrated by RNA silencing , single - cell promoter analysis , and quantitative real - time PCR . -
pubmed
Identification of a
protein hydrolysate responsive
G protein - coupled receptor in enterocytes . -
pubmed
Although such a role for
GPCRs has been implicated in the intestinal response to
protein hydrolysate , no
GPCR directly involved in this process has been previously identified . -
pubmed
In the present study , for the first time , we identified
GPR93 expression in enterocytes and demonstrated its activation in these cells by
protein hydrolysate with EC50 of 10.6 mg / ml as determined by the induction of intracellular free
Ca2 + . -
pubmed
In enterocytes ,
GPR93 was synergistically activated by
protein hydrolysate in combination with an agonist ,
oleoyl - l - alpha - lysophosphatidic acid (
LPA ) , which activated the receptor in these enterocytes with EC50 of 7.9 nM. -
pubmed
The increased intracellular
Ca2 + by
GPR93 activation was observed without the addition of a promiscuous
Galpha protein and was
pertussis toxin sensitive , which suggests
Galpha ( q )- and
Galpha ( i )-mediated pathways . -
pubmed
Both
nuclear factor of activated T cells and
12 - O - tetradecanoylphorbol 13 - acetate responsive elements reporter activities were induced by
protein hydrolysate in cells exogenously expressing
GPR93 . -
pubmed
The peptidomimetic
cefaclor by itself did not activate
GPR93 but potentiated the
protein hydrolysate response and further amplified the synergistic enhancement of
GPR93 activation by
protein hydrolysate and
LPA . -
pubmed
Insulin did not affect its
serotonin content , whereas
serotonin increased its
insulin content at each concentration studied ( down to 10(-21)M ) . -
pubmed
We determined if
serotonin ,
glutamate decarboxylase (
GAD67 ) , or the
kappa opioid receptor (
KOR ) were differentially located within these cell types as predicted by previous studies . -
pubmed
Fixed sections were processed for detection of
biotinamide and immunfluorescence either for
serotonin or for
KOR and
GAD67 . -
pubmed
The product analysis using HPLC , fluorimetry , and
MS revealed the formation of
thiol and
nitric oxide as the major end products . -
pubmed
The study of the interactions between these oligonucleotides and
ACTD at 25 degrees C showed the formation of an interaction complex with a stoichiometry of 1:1 (
ACTD : duplex ) and values for the log(formation constant ) of 5.1+/-0.3 , 6.4+/-0.2 , and 5.6+/-0.2 , respectively . -
pubmed
The aim of this study was to investigate whether
L - Cys supplementation prevents modulation of the activities of erythrocyte membrane
acetylcholinesterase (
AChE ) ,
Na (+) , K (+)- ATPase and
Mg ( 2 +)- ATPase induced by free radicals in basketball players during training . -
pubmed
Na(+),
K (+)- ATPase activity was also higher
post - exercise ( 1.27+/-0.05 micromol Pi / h x mg protein ) than that pre - exercise ( 0.58+/-0.04 micromol Pi / h x mg protein , p<0.001 ) . -
pubmed
When the players were supplemented with
L - Cys , both
AChE and
Na(+),
K (+)- ATPase activities remained unaltered
post - exercise . -
pubmed
Endothelial progenitor cells stimulate cerebrovascular production of
prostacyclin by paracrine activation of
cyclooxygenase-2 . -
pubmed
The increased production of
PGI ( 2 ) and arterial content of
cAMP were inhibited only by a selective
cyclooxygenase-2 inhibitor ,
NS-398 . -
pubmed
In vitro or in vivo treatment of basilar artery with conditioned media from EPCs also caused increase in
cyclooxygenase-2 and
PGI ( 2 ) synthase protein expression associated with elevation of
cAMP . -
pubmed
This effect appears to be mediated by activation of
arachidonic acid metabolism via stimulation of
cyclooxygenase-2 /
PGI ( 2 ) synthase pathway . -
pubmed
The median ( interquartile range ) of the highest total
bilirubin level was 18 ( 15.8 - 20 ) mg / dL and 18.4 ( 16.3 - 19.5 ) mg / dL in normal
G6PD and
G6PD - deficient newborns , respectively ( p - value equals 0.7 ) . -
pubmed
Cultured bacteria differentially expressed
IpLA7 in response to changes in temperature , pH and concentration of
4,5 - dihydroxy-2,3 - pentanedione , the precursor of autoinducer 2 , indicative of mechanisms governing
IpLA7 expression . -
pubmed
This
phosphatase was characterized as a marker for cellular stimulation by its high correlations with induced changes of extracellular pH : 10microM
nigericin ( correlation coefficient r=0.91 ) , 100microM
salicylic acid ( r=0.84 ) , heat
shock 5min 37 degrees C ( r=0.79 ) , and heat
shock after pre - treatment with 5microM
fusicoccin ( r=0.92 ) or 0.5 %
ethanol ( r=0.90 ) . -
pubmed
The Golgi apparatus inhibitor
Brefeldin A ( 200microM ) reduced the heat -
shock - induced
phosphatase ( -33 % ) . -
pubmed
Thus ,
heat - shock - induced phosphatase was characterized by alkaline activity with inhibitors ( 10microM
molybdate : -52 % , 2.5mM
phosphate : -64 % , 10microM
ZnCl ( 2 ): -82 % ) , substrates ( 2.5mM ,
tyrosine phosphate : 255pkat g(-1 ) ,
p - nitrophenyl phosphate : 92pkat g(-1 ) ,
serine phosphate : 0 ,
threonine phosphate : 0 ) , Hill coefficient ( nH=1.4 ) indicating two binding sites , and the extent of heat -
shock stimulation (
p - nitrophenyl phosphate : +190 % ,
tyrosine phosphate : +180 % ) . -
pubmed
SDS - PAGE showed a correlation of
alkaline phosphatase with the heat -
shock - induced release of highly N - glycosylated 53kDa protein , detected by
peroxidase - labeled concanavalin A affinoblotting after
endoglycosidase H treatment . -
pubmed
The 53kDa protein showed no in - gel
phosphatase activity after
SDS - PAGE and regeneration treatment , in contrast to a putative dimer ( 105kDa ) . -
pubmed
Leading models propose that the reduction in photosynthesis at elevated temperature is a function of either declining capacity of electron transport to regenerate
RuBP , or reductions in the capacity of
Rubisco activase to maintain
Rubisco in an active configuration . -
pubmed
It is proposed that measurement of the initial slope of the
CO ( 2 ) response of photosynthesis ( the A / C(i ) response ) can be a useful means to screen for
Rubisco activase limitations . -
pubmed
This is because a reduction in the
Rubisco activation state should be most apparent at low
CO ( 2 ) when
Rubisco capacity is generally limiting . -
pubmed
[ A patient with early
Alzheimer ' s disease who showed improvement of cognitive function and cerebral perfusion by combined therapy of
nilvadipine and
PPAR gamma agonists ] . -
pubmed
Nilvadipine ( a
Ca - channel blocker ) ,
telmisartan ( an
angiotension II receptor blocker ) , and
pioglitazone ( an
insulin sensitizer ) were administered for the control of the
hypertension and
diabetes . -
pubmed
Body condition score was greatest for MB and
NM , least for
HF , and intermediate for
NRF ,
MBX , and NMX . -
pubmed
The
NRF survived the longest in the herd , the
HF survived the shortest , and the
NM ,
MB ,
MBX , and NMX were intermediate . -
pubmed
Effects of
tryptophan microenvironment , soluble domain , and vesicle size on the thermodynamics of membrane protein folding : lessons from the transmembrane protein
OmpA. -
pubmed
Wild - type
OmpA exhibits a free energy of unfolding ( DeltaG degrees
H2O ) of 10.5 kcal / mol . -
pubmed
Mutants , containing a single
tryptophan residue at the native positions 7 , 15 , 57 , 102 , or 143 , are less stable than wild - type
OmpA , with DeltaG degrees
H2O values of 6.7 , 4.8 , 2.4 , 4.7 , and 2.8 kcal / mol , respectively . -
pubmed
The effect of the soluble tail on the conformational stability of the transmembrane domain of
OmpA was also investigated via truncated single -
Trp mutants ; DeltaG degrees
H2O values for four of the five truncated mutants are greater by & gt;2.7 kcal / mol relative to the full - length versions , suggesting that the absence of the soluble domain may destabilize the unfolded transmembrane domain . -
pubmed
LC -
MS and NMR spectroscopic studies demonstrate that the process involves a
pyrone tetraene ( 10 ) as key intermediate , whereas the levels of the final
polypropionates ( 6 , 7 and 9 ) are related to each other and show a significant dependence upon light conditions . -
pubmed
TRPs can be grouped into 7 main subfamilies according to structural homology : the
TRPC ( canonical ) ,
TRPV (
vanilloid ) ,
TRPM ( melastatin ) ,
TRPP (
polycystin ) ,
TRPML (
mucolipin ) ,
TRPA (
ankyrin ) , and
TRPN (
NO mechanopotential ) . -
pubmed
In the present study , we tested whether
leptin can protect gut epithelial cells from
methotrexate (
MTX)-induced
intestinal damage . -
pubmed
The AUC after oral
glucose was related to ethnicity , age and
triglycerides ( r 2=0.27 ) ; after WB to ethnicity , age ,
triglycerides , sex and
CRP ( r 2=0.43 ) ; after CCC to age and weight ( r 2=0.18 ) ; and after FL to age and
CRP ( r 2=0.12 ) . -
pubmed
Activity of
creatine kinase (
CK ) ,
lactate dehydrogenase (
LDH ) ,
myeloperoxidase (
MPO )
superoxide dismutase (
SOD ) and level of
malondialdehyde (
MDA ) in plasma and culture medium were measured to assess the degree of
injury and
inflammation of myocardial tissues and cells . -
pubmed
RESULTS :
Calcium channel blockers (
verapamil ,
diltiazem and
nifedipine ) significantly attenuated
myocardial injury , as shown by reduced release of
CK and
LDH , preserved
SOD activity and decreased
MDA production and
MPO activity . -
pubmed
Thinning or
dehiscence of the superior semicircular canal may occur on the middle cranial fossa floor or adjacent to the superior petrosal
sinus (
SPS ) . -
pubmed
Levels of
HbA and
HbB ,
Hb and
hemopexin , a transporter of
heme , were measured . -
pubmed
Hemin increased
HbA ,
HbB and
hemopexin mRNA levels in cultured neurons . -
pubmed
Deferoxamine reduced
hemin - induced neuronal
Hb expression . -
pubmed
ICH increased
HbA and
HbB expression in the brain , which may potentially serve to buffer the
heme released during clot resolution . -
pubmed
Desmosine (
DES ) and
isodesmosine (
IDES ) are both
pyridinium amino acid isomers that serve as cross - linking molecules binding the polymeric chains of
amino acids into
elastin . -
pubmed
In this study , we aimed to evaluate the
resistin levels in premature neonates and the effect of events such as
preterm prelabour rupture of the membranes ( PPROMs ) and the use of antenatal
steroids on these levels . -
pubmed
Babies with PPROMs had significantly higher levels of
resistin ( ( n=30 ) ; 70.7 ( 7.8 to 568.4 ) ng ml(-1 ) ) than babies without
PPROM ( ( n=88 ) ; 25.9 ( 5.5 to 528.9 ) ng ml(-1 ) ) ( P=0.005 ) , and the babies of mothers who received antenatal
steroids had significantly lower
resistin levels ( ( n=44 ) ; 20.8 ( 5.5 to 159.9 ) ng ml(-1 ) ) than the babies of mothers who did not ( ( n=66 ) ; 34.6 ( 7.2 to 568.4 ) ng ml(-1 ) ) ( P=0.015 ) . -
pubmed
There were significant correlations between
resistin and
IL-6 levels and between
IL-6 and
procalcitonin and
CRP levels in babies whose mothers did not receive antenatal
steroids . -
pubmed
Elevated
resistin levels in babies with
PPROM and suppressed levels in babies whose mothers received antenatal
steroids reported in this study might have been observed as a result of the effects of
fetal inflammation on
resistin levels . -
pubmed
Mutations in the
Plasmodium falciparum chloroquine ( CQ ) resistance transporter (
PfCRT ) are major determinants of
verapamil ( VP)-reversible CQ resistance ( CQR ) . -
pubmed
In western blot analysis , the expression of
cleaved caspase-3 and
heat shock protein 70 declined in
Estradiol pretreated group compared to ischemic control group . -
pubmed
Interestingly ,
Estradiol respectively increased and decreased the expression of
Cyclooxygenase ( COX )- 1 and
COX-2 , to a significant extent . -
pubmed
As for compounds affecting endocannabinoid levels in living tissues , many classes of selective and , in some cases , drug - like inhibitors of
FAAH are available , while only compounds with poor selectivity or in vivo activity are known to inhibit other enzymes involved in endocannabinoid catabolism , such as
NAAA or
MGL , and in endocannabinoid biosynthesis . -
pubmed
Mitochondrial targeting by use of lipid nanocapsules loaded with SV30 , an analogue of the small - molecule
Bcl-2 inhibitor
HA14 - 1 . -
pubmed
The screen also identified broad - spectrum
importin alpha / beta inhibitors such as
ivermectin , which may represent useful tools for nuclear transport research in the future . -
pubmed
The authors validate the activity and specificity of
mifepristone and
ivermectin in inhibiting
nuclear protein import in living cells , underlining the utility of the screening approach . -
pubmed
influenzae were collected and
beta - lactamase - negative isolates were screened for reduced
ampicillin susceptibility using an
ampicillin 2 mug disc ( breakpoint & lt;17 mm ) and Etest ( breakpoint & gt;=0.25 mg / L ) . -
pubmed
All screen - positive isolates had their
ampicillin MICs determined by reference broth microdilution and their
ftsI genes were sequenced . -
pubmed
Enantiomeric , ferromagnetically coupled decanuclear {
Ni6Fe4 } cages with
adamantane - like cores were synthesized around templating
tetraethylammonium cations , as shown by crystallographic analysis and CSI -
MS , and their homochiral nature was confirmed by circular dichroism measurements . -
pubmed
The multiple branching was achieved by breaking the
face - centered - cubic lattice symmetry of gold through
copper - induced formation of single or double twins , and the resulting gold nanocrosses exhibited pronounced near - IR absorption with a great extension to the mid - IR region . -
pubmed
GLYX-13 , a
GluN2B - preferring , NMDAR
glycine - site partial agonist ( 1 mg / kg , i.v . ) -
pubmed
significantly increased positive emotional learning whereas the
GluN2B receptor - specific antagonist ,
ifenprodil ( 10 mg / kg , i.p . ) -
pubmed
Using the RAFT polymerization technique in combination with the reactive ester approach , here we have synthesized well - defined and narrowly distributed
N -( 2 - hydroxypropyl ) methacrylamide homopolymers (
pHPMA ) (
P1 * and
P2 * ) and random
HPMA copolymers consisting of hydrophilic
HPMA and hydrophobic
lauryl methacrylate comonomers (
P3 * and P4 * ) . -
pubmed
Western blot analysis followed by quantitative analysis of respective immunoblot data for
AANAT protein , radioimmunoassay of
melatonin , and spectrophotometric analysis of
Ca ( 2 + ) in the pineal revealed stimulatory effects of both adrenergic (
alpha ( 1 ) and beta ( 1 ) ) and dopaminergic ( D(1 ) ) agonists and cholinergic ( both nicotinic and muscarinic ) antagonists , inhibition by both adrenergic and dopaminergic antagonists and cholinergic agonists , but independent of the influence of any agonists or antagonists of
alpha ( 2 )- adrenergic receptors . -
pubmed
Band intensity of
AANAT and concentration of
melatonin in the pineal were also enhanced by the intracellular
calcium - releasing agent , activators of both
calcium channel and
adenylate cyclase , and
phophodiesterase inhibitor , but suppressed by inhibitor of
calcium channel and
adenylate cyclase as well as activator of
phophodiesterase . -
pubmed
Moreover , an inhibitory effect of light on the pineal
AANAT and
melatonin was blocked by both
cAMP and proteasomal proteolysis inhibitor
MG132 . -
pubmed
Cytokine levels were determined in blood and saliva using ELISA , three times after the administration of
methotrexate and only once in the control group . -
pubmed
RESULTS : The following statistically significant differences were seen : control group pre- vs
post - treatment wave I -
IV interval values ( p & lt ; 0.05 ) ; control vs
vitamin E and B groups ' I -
IV interval values ( p & lt ; 0.05 ) ; control vs other groups ' hearing thresholds ;
vitamin E vs
vitamin B and C and
L - carnitine groups ' hearing thresholds ( p & lt ; 0.05 ) ; and
vitamin B vs
vitamin C and
L - carnitine groups ' hearing thresholds ( p & lt ; 0.05 ) . -
pubmed
Kinetics of advanced glycation end products formation on
bovine serum albumin with various reducing
sugars and
dicarbonyl compounds in equimolar ratios . -
pubmed
Comparing different concentrations of
BSA , we applied various reducing
sugars and reactive
dicarbonyl compounds as AGE - inducing agents at different concentrations . -
pubmed
The NACE -
MS procedure was successfully applied for the analysis of real samples from
seizures in illegal
fentanyl laboratories . -
pubmed
We found that 3 - month
L - arginine supplementation resulted in significant decrease of HOMA - IR and
insulin concentration . -
pubmed
Since the depolarization is initiated by the inhibition of a
TASK - like
K (+ ) channel , we studied if
CIH may increase the amplitude of the
hypoxic - induced depolarization in the chemoreceptor cells , due to an enhanced inhibition of the
TASK - like current . -
pubmed
Present results show that acute hypoxic inhibition of
TASK - like
K (+ ) channel is potentiated by CIH exposure , suggesting that the enhancing effect of CIH on CB chemosensory responsiveness to
hypoxia occurs at the initial step of the
oxygen transduction in the CB chemoreceptor cells . -
pubmed
The
COMT rs4680
Met /
Met genotype was associated with higher impulsivity on the BIS-11 second - order factor Non - planning . -
pubmed
The results suggest that a reduction in
anthocyanin concentration causes the
MRB - G sport . -
pubmed
Transcript levels of
PcUFGT ( for
UDP - glucose : flavonoid 3 - O - glucosyltransferase ) , the key structural gene in
anthocyanin biosynthesis , paralleled the change of
anthocyanin concentration in both
MRB and
MRB - G fruit . -
pubmed
The infiltrated fruits showed blocked
anthocyanin biosynthesis , higher methylation of the
PcMYB10 promoter , and lower expression of
PcMYB10 and
PcUFGT . -
pubmed
Vitamin D exerts its effect through
vitamin D receptor (
VDR ) . -
pubmed
Our data suggest that single
nucleotide polymorphisms ( SNPs ) in
FokI may be possibly involved in longevity and cognitive function . -
pubmed
The purpose of our investigation was to compare the effect of RYGB , vertical sleeve gastrectomy ( VSG ) , and duodenal switch (
DS ) on
insulin and
glucose response to
carbohydrate challenge . -
pubmed
Homoeostatic Model Assessment ( HOMA)-IR , fasting to 1 - h and 1- to 2 - h ratios of
glucose and
insulin , were calculated . -
pubmed
All operations caused
weight loss (
BMI 47.7 + - 10 - 30.7 + - 6.4 kg / m(2 ) in RYGB ; 45.7 + - 8.5 - 31.1 + - 5.5 kg / m(2 ) in VSG ; 55.9 + - 11.4 - 27.5 + - 5.6 kg / m(2 ) in
DS ) , reduction of fasting
glucose , and improved
insulin sensitivity . -
pubmed
CONCLUSIONS : Compared to gastric bypass ,
DS results in greater
weight loss and improves
insulin sensitivity and
glucose homeostasis without causing a
hyperinsulinemic response . -
pubmed
DPMA inhibited microtubule formation and induced expression of
Bax ,
cleaved caspase-3 ,
p53 and ROS , and inhibited
Bcl-2 expression . -
pubmed
DPMA also affected
cyclinB1 ,
cdc2 and
p - cdc2 expression , inducing cell cycle arrest . -
pubmed
These studies demonstrate that
DPMA inhibits
p53 /
cdc2 /
Bax signaling , thereby inhibiting cell growth / angiogenesis and inducing apoptosis . -
pubmed
In contrast , mutant
p62 expression in OCL precursors promotes
receptor activator of NF - kappaB ligand (
RANKL ) hyperresponsivity and increased OCL production , but OCLs that form have normal morphology , are not hyperresponsive to
1,25 - dihydroxyvitamin D3 (
1,25 -( OH ) 2 D3 ) , nor produce elevated levels of
IL-6 . -
pubmed
The main outcome measures were most preferred concentration of salt in broth and crackers ; most preferred concentration of
sucrose in water and jelly ; reported dietary intake of salty and sweet foods ; levels of a bone growth marker ; anthropometric measurements such as height , weight , and percent body fat ; and
TAS1R3 (
sweet taste receptor ) genotype . -
pubmed
The
neurosteroid allopregnanolone ( AP ) is a
GABAA receptor agonist that influences fetal brain development and has neuroprotective properties . -
pubmed
Co - administration of the AP analogue analog
alfaxalone with BM prevented this change in
MBP expression . -
pubmed
The structure - activity relationship (
SAR ) of these
arylpiperazine derivatives was also discussed based on the obtained experimental data . -
pubmed
They showed resistance or intermediate susceptibility to expanded - spectrum
cephalosporins , other
beta - lactams , a
beta - lactam /
beta - lactamase inhibitor combination , and
gentamicin . -
pubmed
ALA concentration and lipid peroxidation in
post - thawed sperm was higher in all treated groups when compared to the control group . -
pubmed
Alkylating agent
melphalan augments the efficacy of adoptive immunotherapy using
tumor - specific
CD4 + T cells . -
pubmed
We showed that
melphalan treatment resulted in a rapid burst of inflammatory
cytokines and
chemokines during the cellular recovery phase after
melphalan - induced myelodepletion and leukodepletion . -
pubmed
After
melphalan treatment ,
tumor cells exhibited characteristics of immunogenic cell
death , including membrane translocation of the endoplasmic reticulum - resident
calreticulin and extracellular release of
high - mobility group box 1 . -
pubmed
These findings provide mechanistic insights into
melphalan 's immunostimulatory effects and demonstrate the therapeutic potential of combining
melphalan with adoptive cell therapy utilizing antitumor
CD4(+ ) T cells . -
pubmed
In addition , multiple linear regression analysis revealed that
ABCG2 rs2231142 had a remarkable effect on
LTG concentrations which is stated to be 4.8 % of the variability of
LTG and may also help to interpret ethnic difference in
LTG pharmacokinetics . -
pubmed
MK-801 ( 100 muM ) , a specific non - competitive antagonist to
NMDA - receptor complex , prevented this effect . -
pubmed
Thus , depending on concentration ,
NMDA can inhibit the synaptic transmission in Schaffer collaterals - hippocampal
CA1 pyramidal neurons axis without triggering the
epileptiform activity . -
pubmed
D - AP5 ( 50 muM ) , a competitive antagonist to
NMDA - receptor complex , completely prevented the inhibitory effect of
NMDA ( 40 muM ) . -
pubmed
Thus ,
MK-801 can prevent the inhibitory action of
NMDA on synaptic transmission in Schaffer collaterals - hippocampal
CA1 pyramidal neurons axis via blocking the channel of
NMDA - receptor complex , while
NMDA exerts its effect only via activation of
NMDA receptors . -
pubmed
Folylpolyglutamate synthetase splicing alterations in
acute lymphoblastic leukemia are provoked by
methotrexate and other chemotherapeutics and mediate chemoresistance . -
pubmed
Anti - HBs antibody development rate by HB vaccination was similar between
HBcrAg - positive and negative patients ( p = 0.325 ) . -
pubmed
The results showed that
ABA concentrations in seeds were low during early development and increased later , peaking at about 50
DAP . -
pubmed
ABA concentrations in
rin accession were similar to those of the control cultivar and thus are not directly associated with the occurrence of vivipary . -
pubmed
SSEA-1 is a marker that detects
malignant thyroid neoplasms in
formalin - fixed paraffin - embedded thyroid tissue sections and may be a useful marker for
thyroid cancer stem - like cells . -
pubmed
The conversion of
arginine to
citrulline residues on histones is catalyzed by
peptidylarginine deiminase 4 (
PAD4 ) . -
pubmed
Here we demonstrate that
Cl - amidine treatment prior to
CLP improves overall survival in
sepsis and the abrogation of
PAD4 has minimal effects on the proinflammatory immune response to
sepsis , while it has no effect on overall
neutrophil migration to the peritoneum . -
pubmed
In addition , both
Si and
Cu ions were released from the
Cu -
BGN in a sustained manner for at least 14days in cell culture medium , indicating the potential of the
BGN as promising carriers for delivering therapeutic
Cu ions . -
pubmed
Release of the
glucose - regulating hormone
insulin from pancreatic islet beta cells is critical for whole - body metabolic homeostasis . -
pubmed
Recent insights indicate a convergence of metabolism - driven signals , such as lipid - derived messengers and redox - dependent deSUMOylation , at the plasma membrane to augment
Ca2 + -dependent
insulin exocytosis . -
pubmed
( ChemRU330 / PPRI 13195 ) had the highest relative
enzyme activity and was tested along with isolates belonging to Hyloscyphaceae ( EdRU083 / PPRI 17284 ) and Leotiomycetes ( EdRU002 / PPRI 17261 ) for
endoglucanase production under different pH and nutritional conditions that included :
carbon sources ,
nitrogen sources and metal ions , at an optimum temperature of 28 C. An optimal of pH 5.0 produced
enzyme activity of 3.99 , 2.18 and 4.31 ( U / mg
protein ) for isolates EdRU083 , EdRU002 and Leohumicola sp . -
pubmed
Increased
enzyme activities and improved mycelial biomass production were obtained in the presence of supplements such as
potassium ,
sodium ,
glucose ,
maltose ,
cellobiose ,
tryptone and
peptone . -
pubmed
The catalytic activity of these NSs towards the PSCB was followed up by thermogravimetric analysis (
TGA ) , where they increased the percentage of total
weight loss , and lowered the decomposition temperatures of
PSCB . -
pubmed
Depupylase Dop Requires Inorganic
Phosphate in the Active Site for Catalysis . -
pubmed
Analogous to eukaryotic ubiquitination , proteins in actinobacteria can be
post - translationally modified in a process referred to as pupylation , the covalent attachment of prokaryotic
ubiquitin - like protein
Pup to
lysine side chains of the target protein via an
isopeptide bond . -
pubmed
However , the enzymes involved in pupylation and depupylation have evolved independently of ubiquitination and are related to the family of
ATP - binding and hydrolyzing
carboxylate -
amine ligases of the
glutamine synthetase type . -
pubmed
Here , we investigate the role played by the
nucleotide in the active site of the
depupylase Dop using a combination of biochemical experiments and X - ray crystallographic studies . -
pubmed
Evidence is presented for a mechanism where the inorganic
phosphate acts as the nucleophilic species in
amide bond cleavage and implications for
Dop function are discussed . -
pubmed
Observed average concentrations of PM2.5 ,
CO , percent relative humidity ( %
RH ) and temperature ( T ) in five different kitchen types were 549.6mug / m3 , 4.2ppm , 70.2 % and 20 C respectively . -
pubmed
Urinary
prednisolone excretion is a determinant of serum
hepcidin levels in renal transplant recipients . -
pubmed
PURPOSE : Recent reports indicate that
histamine and its novel , high - affinity
histamine H4 receptor (
H4R ) play a role in carcinogenesis , and thus
H4R signalling has become a focus of increasing interest in the pathogenesis of many
cancers . -
pubmed
In the periplasm ,
Fdh2 and
hydrogenase Hyn play an important role in reverse electron transport associated with
succinate oxidation . -
pubmed
The
sulfate reduction pathway was poorly regulated and many enzymes associated with
sulfate reduction (
Sat ,
HppA ,
AprAB ,
DsrAB and
DsrC ) were abundant even at conditions where
sulfate was not present . -
pubmed
Hdr /
Flox was detected in all conditions while
HdrABC /
HdrL was exclusively detected when
sulfate was available ; these complexes most likely confurcate electrons . -
pubmed
Nitric oxide (
NO ) production was also significantly suppressed in a dose - dependent manner ( P & lt ; 0.05 ) with an IC50 , the phosphorylation level of
JNK of 3.5 microg / mL. -
pubmed
In the anti - inflammatory pathway studies ,
ERK ,
p38 and
IkappaBalpha were also decreased by
shikonofuran E at 10 muM , in spite of the total levels of the
MAPK isoforms and
IkappaBalpha did not differ significantly . -
pubmed
Amino acid substitution in the C - terminal domain of
collagen XVII reduces
laminin-332 interaction causing mild
skin fragility with
atrophic scarring . -
pubmed
Indeed , the pR1303Q
collagen XVII ectodomain showed decreased binding capability to
laminin-332 and was less co - localized with pericellular
laminin-332 molecules in cell culture . -
pubmed
Compared to high - level serum
zinc group , the risk of
SGA ( OR ( 95%CI ) in low - level serum
zinc group during first trimesters was 1.51 ( 1.05 -
2.19 ) ) . -
pubmed
Density functional theory and
MP2 theoretical approximations predict the existence of two conformers ,
syn - gauche and syn - anti , depending on the value adopted by the dihedral angles
phi ( Cl - C - O - S ) and
phi ( C - O - S - Cl ) . -
pubmed
Early conservative therapy such as bed
rest , legs massage , low fat diet with medium chain
triglycerides , diuretics , bronchodilator inhaler was not be able to satisfy us (
chylous out put & gt ; 330 cc / d ) . -
pubmed
Therefore the catheter replacement with chest tube was carried out followed by pleurodesis using
Talc and
doxycycline besides transition of oral intake to total parentral nutrition and
vitamine E supplement , on
post - operative day = 41 . -
pubmed
Two experimental dentifrices with the same ingredients but different
SnF2 concentrations ( Group
II , 0.4 % w / w ; Group III , 0.454 % w / w ) were used ; distilled water was used in the control group ( Group I ) . -
pubmed
Neuroprotective Effect of Natural Alkaloid
Fangchinoline Against Oxidative
Glutamate Toxicity : Involvement of
Keap1 -
Nrf2 Axis Regulation . -
pubmed
The protective effects of
FAN were almost completely antagonized by inhibitor of
Nrf2 . -
pubmed
Subsequent studies revealed that
FAN could down - regulate
Kelch - like ECH - associated protein 1 (
Keap1 ) in both mRNA level and protein level . -
pubmed
To sum up , our result demonstrated the protective effects of
FAN against
glutamate - induced oxidative
neuronal damage , and for the first time clarified the anti - oxidative mechanisms of
FAN involve activating endogenous antioxidant defense system including enhancing
SOD activity and regulating
Keap1 /
Nrf-2 antioxidation signaling through modulation of
Keap1 expression . -
pubmed
The X - ray crystallographic study confirmed that the title compound crystallizes in the monoclinic
P21 / c space group and possesses a bridging
butyl - ene group that connects two identical
phenyl tetra - zole moieties . -
pubmed
Especially ,
cloquintocet , as a
TP of
cloquintocet - mexyl , had a concentration ratio
TP / parent = 14,809 in effluent . -
pubmed
Survival analyses showed that low
PNI was an independent predictor of short time to treatment failure (
TTF ) [ hazard ratio ( HR ) = 6.87 , P=0.0052 ] , and high NLR ( HR = 3.53 , P=0.0375 ) and high mGPS ( HR = 23.2 , P=0.0038 ) were independent prognostic factors for overall survival ( OS ) after
atezolizumab . -
pubmed
Vascular endothelial growth factor and
pigment epithelial - derived factor in the peripheral response to
ketamine . -
pubmed
There was a significant group x time interaction for
VEGFA mRNA levels ( F(1 , 21 ) = 5.207 , p = 0.029 ) , with
ketamine increasing
VEGFA levels . -
pubmed
There was no significant effect of either
ketamine or
midazolam on
PEDF levels . -
pubmed
There was a significant group x time interaction for
VEGFA /
PEDF mRNA ratio , with
ketamine alone increasing this ratio ( F(1 , 11 ) = 12.085 , p = 0.005 ) . -
pubmed
CONCLUSIONS : These preliminary results support a role for
VEGF in the action of
ketamine and suggest a novel role for
VEGF /
PEDF in the molecular response to
ketamine . -
pubmed
Here we estimate maximal in vivo catalytic rates for 168 plant
enzymes , including
photosystems I and II ,
cytochrome - b6f complex ,
ATP - citrate synthase ,
sucrose - phosphate synthase as well as enzymes from
amino acid synthesis with previously undocumented enzyme kinetic data in
BRENDA . -
pubmed
Binary Logistic regression analysis of SUA /
Cr and
MS and its components correlation were also performed . -
pubmed
A restricted cubic spline model was used to analyze the dose - response relationship between SUA /
Cr and
MS morbidity . -
pubmed
SUA /
Cr positively correlated with the number of
MS components(P<0 . -
pubmed
The correlation intensity between SUA /
Cr value and
MS showed a linear dose - response relationship in euthyroid population(P<0 . -
pubmed
Next , the
oxazoline ring could be broken via the nucleophilic attacking of a water molecular at
C1 position , which generates the final product . -
pubmed
Primary aldosteronism causes secondary erythropoiesis through activation of the
renin -
aldosterone system , but the mechanism is not clear . -
pubmed
Red blood cells ( RBC ) were incubated with the fluorescent
Laurdan probe and median values of Generalized Polarization (
GP ) , representative of membrane fluidity , were compared between the two groups . -
pubmed
After recovery , no differences were observed between
Tris and
ACP -116c that provided 515.4 x 106 sperm / mL and 561.6 x 106 sperm / mL , presenting > 65 % motile sperm , respectively . -
pubmed
Seven patients ( 26.9 % ) had a TE result > = 7.1kPa , which was associated with higher
triglyceride levels , FIB4 score and
CAP values . -
pubmed
An alternative explanation was suggested by the finding that inhibition of locomotion by aggregated
IgG was dependent on the presence of divalent cations at the time the
IgG was added , and did not occur in the presence of the
calcium ionophore
A23187 . -
pubmed
Addition of aggregated
IgG or
chemotactic factors to lymphocytes thus may lead to a gated entry of
calcium , and following closure of the
calcium gate , the cells become relatively unresponsive to further stimulation . -
pubmed
Provisions were made to differentiate ME from
LE by the use of
cyanogen bromide (
CNBr ) to destroy
methionine , the
carboxy terminal
amino acid in the ME sequence . -
pubmed
A comparison of the affinities of
eukaryotic initiation factor 2 and
eukaryotic elongation factor 1 for
GTP and
GDP , and of the responses of initiation and elongation complex formation to various
GTP mol fractions indicated that the initiation reaction was more sensitive to changes in the
GTP :
GDP ratio . -
pubmed
Unexpected occurrence of an aminoacylated
nucleoside in mammalian
tRNATyr . -
pubmed
Each wave is characterized by the emergence , from a pool of growing follicles , of a large dominant follicle which reaches a mature stage of development and may be induced to ovulate and form a functional corpus luteum with a single treatment of either
human chorionic gonadotrophin (
hCG ) or
gonadotrophin - releasing hormone (
GnRH ) . -
pubmed
Such thyroid
antigen - specific T cells have now been shown to recognize one or other of the three
major thyroid - specific antigens ;
thyroglobulin ,
thyroid peroxidase , or the
TSH receptor and efforts are currently in progress to characterize the
T - cell epitopes of these
major thyroid autoantigens . -
pubmed
There is compelling evidence that the thyroid cell itself , by expressing
HLA molecules , and presenting
antigen directly to the T cells , may initiate disease , perhaps after an external insult . -
pubmed
Long - term reduction of
vasopressin excretion induced by the central injection of an immunoconjugate ( antibody to
vasopressin linked to
ricin A chain ) . -
pubmed
In order to investigate the increase in both duration and amplitude of the biological effects seen after the injection of
vasopressin - monoclonal antibody , an immunoconjugate was constructed with the
vasopressin - monoclonal antibody IgG1k isotype and the cytotoxic part of the
ricin molecule , the
ricin A chain . -
pubmed
When non - specific
IgG replaced specific antibody ,
vasopressin excretion , diuresis as well as urine osmolality were unchanged . -
pubmed
However , it acts subsequent to
retinoic acid , and at least one early
retinoic acid - induced event is unaltered in the presence of
LIF . -
pubmed
The events occurring during mammary epithelial cell reorganization on the matrix were shown to be mediated by cell surface
beta-1,4 - galactosyltransferase (
GalTase ) , a receptor that binds
N - acetylglucosamine residues on glycosylated proteins . -
pubmed
The protein
alpha - lactalbumin (
alpha - LA ) inhibits the association of
GalTase with
N - acetylglucosamine . -
pubmed
Cells cultured on plastic , which lack surface
GalTase activity , were unperturbed by incubation with
alpha - LA . -
pubmed
Between nucleotide position 116343 and 116344 two nucleotides -AG- are inserted which prolong the ORF of the
UL56 gene to 233
amino acids with a predicted molecular weight of 30 kDa . -
pubmed
HFI patients exhibit a
deficiency of fructose 1 - phosphate aldolase (
aldolase B ) , the isozyme expressed in tissues that metabolize
fructose . -
pubmed
The viability of the cells was assessed biochemically [ cellular
catecholamine content and the catalytic activities of
tyrosine hydroxylase (
TH ) and
lactate dehydrogenase (
LDH ) ] and anatomically ( by electron microscopy ) . -
pubmed
When cultures of BAMC cells were exposed to
MPTP or
MPP + for 3 days , a marked loss of cellular
catecholamines and
TH activity was observed . -
pubmed
The addition of an inhibitor of
monoamine oxidase ( MAO ) B (
Ro 19 - 6327 ) , but not
MAO A (
clorgyline ) , prevented the
toxicity of
MPTP but not that of
MPP + . -
pubmed
The
toxicity of
MPP + was time dependent , with losses of
TH and the release of cellular
LDH occurring after 48 h in culture . -
pubmed
The rate of
glucose consumption was markedly accelerated by
MPP + treatment and the losses in cell
TH and the release of
LDH into the media were preceded by a 99 % depletion of
glucose from the media . -
pubmed
Culturing cells in
glucose - free medium greatly potentiated the effects of
MPP + on cellular
TH and
catecholamines . -
pubmed
Lithium (
Li ) was shown in therapeutic doses to be a specific inhibitor of the enzyme
inositol monophosphate esterase Li reduces the turnover of
PIP2 and diminishes the cell responses . -
pubmed
In order to investigate this new mode of action of
Li the authors studied the influence of acute dose of
Li on the response of
prolactin (
PRL ) and
thyrotropin (
TSH ) to
thyrotropin stimulating hormone (
TRH ) , a stimulation mainly mediated through the activation of the turnover of
PIP2 . -
pubmed
One such suppressor ,
MSI1 , was found to encode a putative protein of 422
amino acids that shows homology to the beta subunit of the mammalian
guanine nucleotide - binding regulatory proteins . -
pubmed
Analysis of the
psoralen - crosslinking pattern in chromatin DNA by
exonuclease digestion . -
pubmed
The pattern of these crosslinks can be analysed by
exonuclease digestion of random DNA fragments , since the
exonucleases tested stop at sites of
psoralen - crosslinks . -
pubmed
Further digestion of these fragments with
S1 - nuclease leads to DNA fragments of nucleosomal and polynucleosomal size , which presumably carry
psoralen - crosslinks at both ends . -
pubmed
The assay for
UR phosphorylase appeared to be comparable to existing methods with
radiolabeled UR as substrate regarding sensitivity and linearity . -
pubmed
Application of
carbachol or
substance P produced contraction and the adrenergic agonist
isoprenaline or
vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens . -
pubmed
Serum lysozyme and
vitamin B 12 binding capacity in
myeloproliferative disorders . -
pubmed
However , the ratio of
[ 14C ] DMA - labelled
hemoglobin in the sol phase to that in the gel phase in the unfractionated hemolysate was 1.17 at pH 7.4 and 1.25 at pH 8.4 , suggesting that part of the cross - linked
hemoglobin tetramers was incorporated into the gel . -
pubmed
In experiment
II the unit activity of both cortical areas was compared during the free head movement towards
food and during the movement hampered with the rubber thread . -
pubmed
There are several possible explanations for the preincubation effects , such as new synthesis of
carrier protein , altered fluidity of the membrane or stimulation of the
sodium - dependent
taurocholate uptake via a change in the cation distribution . -
pubmed
Since the development peak eluted ahead of the vegetative peak in all but one case , and since levels of
amino acid acceptance and
tRNA chromatographic profiles were otherwise quite similar , it was suggested that a common change or a very small number of changes in base modification were occurring during development . -
pubmed
Indeed , the
erythromycin - resistance marker (
erm ) , originating in
pAM beta 1 , has been used as an indicator of local transcription on several occasions . -
pubmed
To overcome this limitation , new
drug - resistance cassettes were designed and constructed , using pA as a model for synthetic promoters for the
erm and
cat genes . -
pubmed
Erythrocyte filterability ( 5 microns pore size ) was significantly lower ,
fibrinogen concentration significantly higher , and
HDL -
cholesterol concentration significantly lower in the patient group . -
pubmed
No significant differences were found regarding hematocrit , mean corpuscular volume ,
hemoglobin concentration , leukocyte count and filterability ( 8 microns pore size ) , plasma viscosity , and total
cholesterol concentration . -
pubmed
Selective binding of
gelsolin to
actin monomers containing
ADP . -
pubmed
The rate of reaction and the stoichiometry of binding between
gelsolin and
actin monomers depends on
adenine nucleotides . -
pubmed
In the presence of
Ca2 + but not
Mg2 + ,
gelsolin retains the ability to sever
actin filaments when incubated for more than 20 min with an excess of
G - actin in the presence of
ATP but loses severing activity within seconds when mixed with
G - actin in
ADP . -
pubmed
Immunoprecipitation of
gelsolin removes more
actin from
ADP than from
ATP solutions . -
pubmed
Monomeric
ATP -
actin in 2 mM
MgCl2 and 150 mM
KCl slowly destroys the filament - severing activity of
gelsolin with kinetics that are first order in
actin concentration and with an apparent bimolecular rate constant of 0.021 + /- 0.007 microM-1 s-1 . -
pubmed
Coincident with the slow complex formation in
MgCl2 , the
actin bound to the
calcium - sensitive
actin binding domain of
gelsolin hydrolyzes its
ATP to
ADP . -
pubmed
These results suggest a further level of
gelsolin regulation and a functional similarity between
actin and
GTP - binding proteins . -
pubmed
Each of the exon products 7 (
Lys236 - Gln292 ) , 9 (
Val293 - Gly328 ) , and 8 (
Gln329 -
Met357 ) , and their combinations were evaluated for the ability to inhibit
thrombin induced
platelet aggregation . -
pubmed
A deletion mutant of exon 7 product , polypeptide 7A product (
Lys236 -
Lys270 ) did not block
thrombin - induced
platelet aggregation , while
7B product (
Thr255 -
Gln292 ) and 7C product (
Leu271 -
Gln292 ) inhibited aggregation . -
pubmed
Peptides
Phe279 -
Ile283 and
Phe281 -
Gln292 did not block
thrombin , and
Asn275 -
Phe279 had only minimal inhibitory activity . -
pubmed
A heptapeptide
Leu271 -
Ala277 inhibited
thrombin - induced aggregation of platelets with an IC50 of 65 microM. -
pubmed
The effect is specific for the activation of platelets by
thrombin but not
ADP or
collagen . -
pubmed
G - CSF - treatment significantly shortened the optimal time interval of
CPA doses when compared with treatment with the control vehicle . -
pubmed
These results suggest that
G - CSF shortens the optimal time interval between
CPA doses not only by accelerating the recovery of G -
CFC but also by affecting the hemopoietic environment . -
pubmed
DNA sequence determination of the fusion joints revealed an open reading frame (
ORF1 ) , encoding a gene product of 137
amino acids with a typical N - terminal hydrophobic signal sequence . -
pubmed
ORF1 was designated the
glo gene (
G protein - like ORF ) because its
amino acid sequence shows similarity to
eukaryotic Gs ( alpha ) protein ( 34.5 % identity over an 81 - amino - acid overlap ) and its C - terminus displays the consensus motif ( CAAX ) which is found in many small eukaryotic
GTP - binding proteins . -
pubmed
First , the enhancer complex overcomes an inhibitory effect of the
sigma 54 leucine patch and unlocks the melting activity of the holoenzyme . -
pubmed
The
GH receptor (
GHR ) is a protein of 620
amino acids ; the extracellular domain of 246
amino acids is made of two subdomains , one being the domain of interaction with the ligand , the second one being the region of association with another receptor resulting in a
homodimer . -
pubmed
GH ,
insulin and sex
steroids also influence plasma
GHBP levels . -
pubmed
This inhibitory action of
interferon - alpha in
dopamine neurons may be involved in adverse central effects , such as
parkinsonism and
depression with suicidal potential . -
pubmed
Based on this study ,
Poloxamer 407 was found to be the most effective stabilizer for
rhGH for protection against interfacial and thermal stress . -
pubmed
Thyroid hormones also decrease the expression of
phospholamban , but increase the expression of
sarcoplasmic reticulum Ca2 + - pump . -
pubmed
As a result , the basal activity of sarcoplasmic reticulum
Ca2 + -pump increases , but its beta - adrenergic activation through phosphorylation of
phospholamban decreases . -
pubmed
Dopamine and the
P50 . -
pubmed
The swimming training also resulted in an approximately 20 % increase in the
insulin - stimulated
glucose transport activity ( p & lt ; 0.05 ) of soleus muscle strips and an approximately 3 - fold increase in the plasma membrane
GLUT4 protein expression ( p & lt ; 0.01 ) in SHRSP . -
pubmed
However , basal and
insulin - stimulated
glucose transport activity and
GLUT4 contents were not significantly different between WKY and SHRSP . -
pubmed
Uptake of
Tc-99 m sestamibi and
Tc-99 m MDP in
anaplastic carcinoma of the thyroid ( nondiagnostic
CT and ultrasound scans ) . -
pubmed
Clavulanic acid restored the
ceftazidime activity , thus suggesting an extended spectrum
beta - lactamase (
ESBL ) . -
pubmed
After cloning and sequencing , the ESBL ( TEM-29B ) showed one
amino acid replacement with respect to the
TEM-1 sequence ,
Arg-164 to
His . -
pubmed
The objectives were 1 ) to investigate the effects of oocyte maturation in serum - free and
amino acid - supplemented defined media on oocyte transcript levels , blastocyst cell number , and apoptosis ; 2 ) to investigate the influence of oocyte maturation culture atmosphere on blastocyst development , total cell number , and apoptosis ; and 3 ) to examine the influence of
epidermal growth factor (
EGF ) during oocyte maturation on blastocyst cell number and apoptosis . -
pubmed
In this study , we used an inhibitor of
S - adenosylmethionine decarboxylase ,
diethylglyoxal bis ( guanylhydrazone)(
DEGBG ) , to block the synthesis of
spermidine and
spermine from
putrescine . -
pubmed
In addition , the normal distribution of
actin filaments required for migration , which is disrupted in
polyamine - deficient cells , could be achieved by adding
spermine but not
putrescine along with
DFMO and
DEGBG . -
pubmed
Since
IFN - gamma also stimulates the release of reactive
oxygen species ( ROS ) from immunocompetents cells , the amount of
neopterin produced also serves as an indirect estimate of oxidative stress . -
pubmed
In parallel ,
IFN - gamma activates the degradation of
tryptophan , which appears to limit the growth of intracellular pathogens and the proliferation of cells , including T lymphocytes . -
pubmed
VEPs were performed in each case before and after 6 months treatment with either intravenous immunoglobulins (
IVIG ) or
steroids . -
pubmed
The radiation exposure inside the spacecraft in low earth orbit was investigated with a telescope based on two
silicon planar detectors during three NASA shuttle - to -
MIR missions ( inclination 51.6
deg , altitude about 380 km ) . -
pubmed
Measured mission averaged dose rates in
silicon are in the range 98 - 108 microGy d-1 and 137 - 178 microGy d-1 for the GCR and
SAA contributions , respectively . -
pubmed
The specific aim of this study was to determine the basal
cortisol values predicting a normal or impaired response to the
insulin hypoglycemia test ( ITT ) . -
pubmed
The capability of antibodies to
myosin II in abrogating the secretory response from populations of
digitonin - permeabilized cells compared with the modest effect caused by anti -
myosin V suggests that
myosin II plays a fundamental role in the active transport of vesicles occurring in the sub - plasmalemmal area during chromaffin cell secretory activity . -
pubmed
The resulting
amino acid substitution ( K42N ) in
ribosomal protein S12 causes an increased rate of ribosomal proofreading and , as a result , the rate of protein synthesis , bacterial growth and virulence are decreased . -
pubmed
Most of the compensatory mutations , resulting in at least 35 different
amino acid substitutions , were novel single - nucleotide substitutions in the
rpsD ,
rpsE ,
rpsL or
rplS genes encoding the ribosomal proteins
S4 ,
S5 ,
S12 and
L19 respectively . -
pubmed
Effects of
erythropoietin and
pentoxifylline on the oxidant and antioxidant systems in the experimental
short bowel syndrome . -
pubmed
In this study , we investigated the effects of
erythropoietin (
Epo ) , and
pentoxifylline (
Ptx ) on the oxidant and antioxidant systems in the experimental
short bowel syndrome . -
pubmed
Malondialdehyde (
MDA ) ,
superoxide dismutase (
SOD ) and
glutathione peroxidase (
GSH - Px ) levels were determined in ileum homogenates . -
pubmed
When compared to group C , the
MDA and
GSH - Px levels were significantly decreased ( p & lt ; 0.05 ) , but
SOD activity was not changed ( p & gt ; 0.05 ) in groups P and E+P , whereas both
MDA and
SOD and also
GSH - Px activities were not changed significantly in group E ( p & gt ; 0.05 ) . -
pubmed
Therefore , our study indicates that
Ptx may be more effective than
Epo in reducing lipid peroxidation . -
pubmed
PI3K -
Akt is an important survival signal pathway , that has been shown to be the target of various antitumor drugs , including
UCN-01 and
geldanamycin , new anticancer drugs under clinical evaluation . -
pubmed
Dopamine transporter change in drug - naive
schizophrenia : an imaging study with
99mTc - TRODAT-1 . -
pubmed
Correlation of specific
MPZ mutations with their phenotypes demonstrated that addition of either a charged
amino acid or altering a
cysteine residue in the extracellular domain caused a severe early onset
neuropathy . -
pubmed
The effectiveness and efficiency of the tunable incremental factor algorithm is exemplified in the experimental results on clinical
indocyanine green angiograms , and the convexity of the
SSD function under ideal imaging condition is also illustrated in the paper . -
pubmed
Long chain
acyl - CoA dehydrogenase (
LCAD ) has recently been shown to be the mitochondrial enzyme responsible for the beta - oxidation of branched chain and
unsaturated fatty acids [ Biochim . -
pubmed
To identify the generators of the aSSR ,
15O - water PET imaging was used to locate the regions of the brain activated by a steady 1 kHz pure tone , the same tone amplitude modulated (
AM ) at 40 Hz and the specific regions of the brain responsive to the
AM component of the stimulus relative to the continuous tone . -
pubmed
The chemical mechanism by which the
enzyme nitrogenase effects the remarkable reduction of
N ( 2 ) to
NH ( 3 ) under ambient conditions continues to be enigmatic , because no intermediate has been observed directly . -
pubmed
Recent experimental investigation of the enzymatic consequences of the
valine --> ;
alanine modification of residue
alpha-70 of the component
MoFe protein on the reduction of
alkynes , together with
EPR and ENDOR spectroscopic characterization of a trappable intermediate in the reduction of
propargyl alcohol or
propargyl amine (
HCC [ triple bond ] C - CH ( 2 ) OH /
NH ( 2 ) ) , has localized the site of binding and reduction of these substrates on the
FeMo - cofactor and led to proposed
eta ( 2 )- Fe coordination geometry . -
pubmed
Here these experimental data are modeled using density functional calculations of the
allyl alcohol /
amine intermediates and the
propargyl alcohol /
amine reactants coordinated to the
FeMo - cofactor , together with force - field calculations of the interactions of these models with the surrounding
MoFe protein . -
pubmed
Bradykinin mediates acute
inflammation by increasing microvascular permeability , vasodilation , leukocyte migration and accumulation , and the production of
arachidonic acid via
phospholipase A2 activation . -
pubmed
Bradykinin exerts its inflammatory effects via the
bradykinin B2 receptor . -
pubmed
The aim of this study was to evaluate the effect of a
bradykinin B2 receptor antagonist ,
FR173657 ( FR ) , on intestinal
ischemia -
reperfusion ( I / R ) injury . -
pubmed
The serum
thromboxane B2 and
6 - keto - prostagland in F1alpha levels were significantly lower in Group
II than in Group I ( P & lt ; 0.05 ) . -
pubmed
We conclude that
FR treatment appears to have clear protective effects on small bowel I / R
injury by inhibiting the release of
eicosanoids . -
pubmed
BACKGROUND AND
AIM : So far , no studies have assessed whether there is an association between
iron status and the incidence of major adverse cardiac events or restenosis after coronary stenting . -
pubmed
The
soluble transferring receptor /
ferritin ratio (
sTfR /
ferritin ratio ) was used as an index of
iron status . -
pubmed
The authors assessed 15 - year ( 1985 - 2001 ) incident
diabetes ( 148 cases ) ,
insulin concentration , and
insulin resistance ( homeostasis model assessment ) in smokers and nonsmokers according to baseline levels of serum
alpha - carotene ,
beta - carotene ,
zeaxanthin ,
beta - cryptoxanthin , and
lycopene . -
pubmed
It was found that in this model , all formulations of HA and the majority of DPCC lubricants reduced friction (
HA 5 and 10 mg ml(-1 ) ,
DPPC 200 mg ml(-1 ) reductions of 51.9 % , 46.7 % and 46.5 % respectively ) , compared to a Ringers solution control . -
pubmed
By combining HA and
DPCC ( HA /
DPPC ; 5 mg ml(-1)/100 mg ml(-1 ) and 10 mg ml(-1)/200 mg ml(-1 ) ) , a further improvement was noted ( 69.5 % and 61.9 % , respectively ) as the mean levels of friction were reduced by up to a further 17 % than the most effective individual formulation (
HA 5 mg ml(-1 ) ) . -
pubmed
Cyclooxygenase-2 selective NSAIDs such as
celecoxib may be less likely to promote
gastrointestinal bleeding ; however , there are concerns that they could potentiate the anticoagulation effect of
warfarin . -
pubmed
Using site - directed mutagenesis , two
cysteine residues were introduced in close spatial proximity (
P5C / A135C ) . -
pubmed
Chlorophenols ( CPs ) were employed as model compounds to assess the extraction procedure and were determined by gas chromatography - electron - capture detection (
GC -
ECD ) . -
pubmed
In solid - phase extraction - dispersive liquid - liquid microextraction ( SPE - DLLME ) , CPs were adsorbed from a large volume of aqueous samples ( 100 mL ) into 100 mg functionalized
styrene -
divinylbenzene polymer (
PPL ) sorbent . -
pubmed
This study developed and validated a positive - ion mode liquid chromatography / tandem mass spectrometry ( LC /
MS /
MS ) method for the quantitative determination of the major (
synephrine ) and minor (
tyramine ,
N - methyltyramine ,
octopamine , and
hordenine ) adrenergic protoalkaloids in a suite of National Institute of Standards and Technology ( NIST ) bitter orange Standard Reference Materials ( SRMs ):
SRM 3258 Bitter Orange Fruit ,
SRM 3259 Bitter Orange Extract , and
SRM 3260 Bitter Orange Solid Oral Dosage Form . -
pubmed
LC /
MS /
MS analysis of the
protoalkaloids in the SRMs resulted in mean measurement imprecision levels of & lt ; or = 10 % coefficient of variation ( % CV ) . -
pubmed
The
PfEMP1(
SM ) are encoded by group A
var genes that are composed of a more constrained range of
amino acid sequences than groups B and C
var genes encoding
PfEMP1(
UM ) associated with
uncomplicated malaria . -
pubmed
Aldosterone increases oxidant stress to impair
guanylyl cyclase activity by
cysteinyl thiol oxidation in vascular smooth muscle cells . -
pubmed
Soluble
guanylyl cyclase (
GC ) is a heterodimer that is activated by
NO ( . ) -
pubmed
In VSMC treated with
aldosterone , Western immunoblotting detected evidence of
GC beta(1)-subunit
disulfide bonding , whereas mass spectrometry analysis of a homologous peptide containing the
Cys-122 - bearing sequence exposed to conditions of increased oxidant stress confirmed
cysteinyl sulfinic acid ( m / z 435 ) ,
sulfonic acid ( m / z 443 ) , and
disulfide ( m / z 836 ) bond formation . -
pubmed
Exposure to
aldosterone or
hydrogen peroxide (
H ( 2 ) O ( 2 ) ) significantly decreased
cGMP levels in cells expressing wild - type
GC . -
pubmed
In contrast ,
aldosterone or
H ( 2 ) O ( 2 ) did not influence
cGMP levels in cells expressing the mutant C122A
GC , confirming that oxidative modification of
Cys-122 specifically impairs GC activity . -
pubmed
These findings demonstrate that pathophysiologically relevant concentrations of
aldosterone increase oxidant stress to convert
GC to an
NO(. -
pubmed
Six
14C - radiolabeled chemicals ranging in lipophilicity were tested , including
benzo [ a ] pyrene (
BaP ) ,
ethylene glycol ( EG ) ,
methyl parathion ( MP ) ,
naphthalene (
Nap ) ,
nonyl phenol (
NP ) , and
toluene (
Tol ) . -
pubmed
Recently , the blockade of
acid - sensing ion channels (
ASICs ) with
amiloride and
A-316567 attenuated the reflex . -
pubmed
Moreover ,
amiloride had no effect on the mechanoreceptor component of the reflex , prompting us to determine whether
ASICs contributed to the metaboreceptor component of the exercise pressor reflex . -
pubmed
Also ,
TCDD group had significantly lower thymic and splenic weights , decreased percentages of
CD4(+)
CD8(+ ) thymocytes and splenic
CD4(+ ) T cells , increased percentage of splenic
B220(+)
sIgM(+ ) B cells and higher serum
interferon gamma concentration . -
pubmed
METHODS : We established a quantitative method to determine covalent binding mediated by
aldehydes via
hemiaminal or
hemiacetal using non - radiolabeled compound and
[ ( 14 ) C ] semicarbazide as a hard - trap agent with unique
post - incubation . -
pubmed
RESULTS : The trapped
aldehyde obtained from the
post - incubation was almost equivalent to the covalent binding of the radiolabeled tool compound . -
pubmed
DISCUSSION : The
post - incubation method is useful for screening newly synthesized compounds to quantitatively assess the bioactivation of
aldehydes descending from
alicyclic amines . -
pubmed
In addition to a questionnaire ,
body mass index ( BMI ) , blood pressure , fasting blood
glucose , lipids ,
albumin and serum
DHEA - S levels were measured for each participant . -
pubmed
Using multivariate logistic regression analyses with adjustments for age , smoking ,
alcohol , physical activities ,
albumin and
BMI , there was a positive relationship between serum
DHEA - S level and
metabolic syndrome . -
pubmed
Post - translational modification of
histone such as acetylation of N - terminal of
lysine residues influences gene expression by modulating the accessibility of specific transcription factors to the promoter region , and is essential for a wide variety of cellular processes in the development of individual tissues , including the brain . -
pubmed
Systematic examination of protein depositions revealed parenchymal
amyloid - beta in 53.8 % ,
amyloid angiopathy (
Abeta ) in 23.1 % ,
phospho -
tau immunoreactive neuritic profiles in 92.3 % ,
neurofibrillary degeneration in 38.4 % , new types of
tau pathology in 33.3 % , and Lewy - type
alpha - synuclein pathology in 15.4 % . -
pubmed
These results show that the extracts , fractions , and isolated
C - glycosylflavones have an antihyperglycemic action that was reinforced by the stimulation on in vivo
insulin secretion . -
pubmed
Along with the elevated
ASM activity , there also was an elevation of
acid ceramidase (
AC ) activity , which was sustained for up to 14 days
post -
bleomycin treatment . -
pubmed
CONCLUSIONS : These data demonstrate that the
sphingomyelin /
ceramide signaling pathway is involved in the pathogenesis of
bleomycin - induced
pulmonary fibrosis , and suggest that inhibition of
ASM may potentially slow the fibrotic process in the lung . -
pubmed
An alternative , one -
pot route that avoids use of the highly air - sensitive complex 1 is described for the synthesis of the
iron ( II )- lithium complex
[ { ( Me ( 3 ) Si )( 2 ) N }( 2 ) Fe { Li ( bta )}]( 2 ) ( 3 ) ( where btaH =
benzotriazole ) , in which both
iron ( II ) centers reside in 3 - coordinated pyramidal environments . -
pubmed
Suspect
TBM if there is a
CSF leucocytosis ( predominantly lymphocytes ) , the
CSF protein is raised , and the CSF plasma
glucose is & lt;50 % . -
pubmed
As expected , surgery was accompanied by a reduction in serum
calcium ( from 2.77 + - 0.2 to 2.25 + - 0.1 mmol / L , p<0.001 ) and
parathyroid hormone ( from 29.6 + - 10 to 3.3 + - 2 pmol / L , p<0.001 ) . -
pubmed
Inhibitory analysis with
TG2 - specific inhibitor
KCC-009 suggests that relative contribution of
TG2 in total transamidating activity differs in various tissues . -
pubmed
The study of
PITPs is fundamental to understanding of how the
phosphoinositide signal transduction network is regulated and integrated to the larger lipid metabolome in diverse cellular processes . -
pubmed
To comprehend how the
PITPs integrate
phosphoinositide signaling to broader lipid metabolome in diverse cellular processes , it is necessary to devise methods that can correlate the biochemical properties of these non - enzymatic proteins to biologically relevant functional insights . -
pubmed
Multimodular
polyketide synthases (
PKSs ) have an assembly line architecture in which a set of protein domains , known as a module , participates in one round of
polyketide chain elongation and associated chemical modifications , after which the growing chain is translocated to the next
PKS module . -
pubmed
RecQ5 interacts with
Rad51 and is involved in resistance of Drosophila to
cisplatin treatment . -
pubmed
Moreover , the C - terminal region of the
RecQ5 protein and the central region of
Rad51 interacted directly and specifically when examined by the
glutathione - S - transferase pull - down method . -
pubmed
Furthermore ,
RecQ5 - deficient flies (
recq5 ) were more sensitive to the chemotherapeutic agent
cisplatin compared with wild - type ones . -
pubmed
Also ,
Rad51 mutants (
rad51 ) were more sensitive to
cisplatin , with sensitivity similar to that of
recq5 rad51 double mutants . -
pubmed
Sensitive layer - by - layer ( LbL ) coatings for optical detection of gaseous
NH ( 3 ) and
HCl were prepared by self - assembly of oppositely charged polysaccharides ( chitosan and lambda - carrageenan ) followed by doping LbLs with pH - sensitive dyes -
bromothymol blue (
BTB ) and
Congo red (
CR ) . -
pubmed
CR - doped OWG sensors have shown high sensitivity to
HCl vapor but slow relaxation time ( up to several hours for 12 DL LbL films ) . -
pubmed
After each 3 - week period , a standardized fat - rich meal was provided , and the effects of
liraglutide on
triglyceride ( primary endpoint AUC(0 - 8h ) ) ,
apolipoprotein B48 , non - esterified
fatty acids , glycaemic responses and gastric emptying were assessed . -
pubmed
FUNDING : Novo Nordisk A / S. RESULTS : After 3 weeks , mean postprandial
triglyceride ( AUC(0 - 8h )
liraglutide / placebo treatment - ratio 0.72 , 95 % CI [ 0.62 - 0.83 ] , p = 0.0004 ) and
apolipoprotein B48 ( AUC(0 - 8h ) ratio 0.65 [ 0.58 - 0.73 ] , p & lt ; 0.0001 ) significantly decreased with
liraglutide 1.8 mg versus placebo , as did iAUC(0 - 8h ) and C(max ) ( p & lt ; 0.001 ) . -
pubmed
Mean
low - density lipoprotein and total
cholesterol decreased significantly with
liraglutide versus placebo . -
pubmed
X - ray diffraction ( XRD ) and transmission electron microscopic (
TEM ) results establish formation of partially intercalated and partially exfoliated HPMC /
MMT nanocomposite films . -
pubmed
Using Molecular Mechanics and Molecular Dynamics methods , we investigated at the atomistic level the topography effects both on physisorption on different crystalline planes of
TiO2 anatase and on the competitive adsorption when three different crystallographic
faces were simultaneously present in an idealized nanosized crystal interacting with a simple
heteroaromatic molecule experimentally used in sunlight - induced photosynthetic reaction . -
pubmed
Re - irradiation was performed a median of 5.8 years after initial RT , and delivered using lateral opposed beams ( n = 1 ) , IMRT ( n = 4 ) , linear - accelerator based
SRS ( n = 3 ) , FSRT ( n = 3 ) , gamma knife surgery ( n = 2 ) , and
yttrium-90 brachytherapy ( n = 1 ) . -
pubmed
Simulations of
EPR spectra have revealed the presence of several types of radicals (
carbon - centred ,
tyrosyl and
semiquinone ) localized in starch and protein fractions of flours . -
pubmed
It has been proposed that the canonical 30 nucleotide single - stranded DNA gap generated by
nucleotide excision repair is the signal that activates the
ATR - mediated DNA damage checkpoint response and that the signal is enhanced by gap enlargement by
EXO1 (
exonuclease 1 ) 5 ' to
3 ' exonuclease activity . -
pubmed
Here we have used purified core
nucleotide excision repair factors (
RPA ,
XPA ,
XPC ,
TFIIH ,
XPG , and
XPF -
ERCC1 ) , core DNA damage checkpoint proteins (
ATR -
ATRIP ,
TopBP1 ,
RPA ) , and DNA damaged by a UV - mimetic agent to analyze the basic steps of DNA damage checkpoint response in a biochemically defined system . -
pubmed
[ Design , synthesis and evaluation of
bis - nicotine derivatives as inhibitors of
cholinesterases and
beta - amyloid aggregation ] . -
pubmed
The pharmacological results indicated that compounds 3e-3i inhibited both
acetylcholinesterase (
AChE ) and
butyrylcholinesterase (
BChE ) in the micromolar range ( IC50 , 2.28 - 117.86 micromol x L(-1 ) for
AChE and 1.67 - 125 micromol x L(-1 ) for
BChE ) , which was at the same potency as
rivastigmine . -
pubmed
Meth directly stimulated the excitatory node , an inhibitory drive targeted the medullary node , and , in high doses , an additional excitatory drive affected the
SPN node . -
pubmed
While
CtBP1 and
CtBP2 share significant
amino acid sequence homology ,
CtBP2 possesses a unique N - terminal domain that is modified by acetylation and contributes to exclusive nuclear localization . -
pubmed
The purified
RpXDH preferred
NAD(+ ) as its coenzyme and showed substrate specificity for
xylitol ,
d - sorbitol , and
ribitol . -
pubmed
Our results indicate that
FRbeta is a promising target for CAR T - cell therapy of
AML , which may be augmented by combination with
ATRA . -
pubmed
Apolipoprotein C - II (
apoC - II ) is the co - factor for
lipoprotein lipase (
LPL ) at the surface of
triacylglycerol - rich lipoproteins . -
pubmed
LPL hydrolyzes
triacylglycerol , which increases local surface pressure as surface area decreases and amphipathic products transiently accumulate at the lipoprotein surface . -
pubmed
ApoC - II adsorption to a
triacylglycerol / water interface resulted in large increases in surface pressure . -
pubmed
We characterized
apoC - II at phospholipid /
triacylglycerol / water interfaces , which more closely mimic lipoprotein surfaces . -
pubmed
ApoC - II is provided as a component of
triacylglycerol - rich lipoproteins and is the co - factor for
LPL as pressure increases . -
pubmed
The serum ionic concentration in each group was tested by turbidimetric method ;
hematoxylin - eosin staining was used to measure the severity of
cochlea hydrops ; immunohistochemical method was used to observe the expression of
AQP1 in the cochlea . -
pubmed
Combination of UV - B and metals seems more detrimental to the
NH4 (+ ) ,
PO4 ( 3 - ) uptake ,
GS and APA activity . -
pubmed
A significant stimulation in
NO3 (- ) uptake and
NR activity following exposure to UV - B in all the three cyanobacteria suggests UV - B - induced structural change(s ) in the
enzyme / carriers . -
pubmed
BACKGROUND :
Anoctamin 5 (
ANO5 ) is a member of a conserved gene family (
TMEM16 ) , which codes for proteins predicted to have eight transmembrane domains and putative
Ca ( 2 +)-activated
chloride channel (
CaCC ) activity . -
pubmed
The fo properties were correlated to the average serum levels of bioavailable
estradiol (
E2 ) ( mean
E2 /
sex hormone - binding globulin [
SHBG ] ) and
testosterone ( T ) ( mean T /
SHBG ) across the second month of life . -
pubmed
AIM : The growing implementation of
iron oxide nanoparticles in medicine requires a thorough investigation of their physiological influence . -
pubmed
These include the alteration of neuronal excitability via specific
antigens such as the
voltage - gated potassium channel complexes or by mediating bone destruction in
rheumatoid arthritis . -
pubmed
Mechanisms to explain these
cholesterol - related changes are multiple , including alterations in tenocyte gene and protein expression , matrix turnover , tissue vascularity , and
cytokine production . -
pubmed
BACKGROUND :
BAG3 is a 575
amino acid protein that has pleotropic functions in the cell including pro - autophagy and anti - apoptosis . -
pubmed
The salutary benefits of rAAV9 -
BAG3 were also observed in myocytes isolated from
MI hearts including improved cell shortening ( p<0.05 ) , increased systolic [
Ca2+]i , increased [
Ca2+]i transient amplitudes and increased maximal ICa amplitude . -
pubmed
decreased activities of anti - oxidant armory (
SOD ,
CAT ,
GPx ,
GR ) and
GSH content . -
pubmed
MTX significantly causes upregulation of
iNOS ,
Cox-2 ,
Bax and downregulation of
Bcl-2 expressions , it causes higher
caspase 3 , 9 activities . -
pubmed
CGA inhibited
Cox-2 ,
iNOS ,
Bax ,
Bcl-2 and
Caspases 3 ,
9 mediated inflammation and apoptosis , and improve the histology induced by MTX . -
pubmed
The inhibition of
thrombin and
factor X activities , mediated through
antithrombin III and heparin cofactor
II of fCSs
oligosaccharides were affected by their molecular weight and
fucose branches . -
pubmed
Oligosaccharides with different sulfation patterns of the
fucose branching had a similar ability to inhibit the
FXa by the intrinsic
factor Xase (
factor IXa - VIIIa complex ) . -
pubmed
Furthermore , a
heptasaccharide is the minimum size
oligosaccharide required for anticoagulation and
FXII activation . -
pubmed
Molecular size and
fucose branch sulfation are important for anticoagulant activity and reduction of size can reverse the activation of
FXII caused by native fCSs . -
pubmed
CYP2D6 and
Endoxifen in
Tamoxifen Therapy : A Tribute to David A. Flockhart . -
pubmed
Post - Exercise Ingestion of Low or High Molecular Weight
Glucose Polymer Solution Does Not Improve Cycle Performance in Female Athletes . -
pubmed
The current study sought to evaluate the effects of
post - exercise ingestion of a high molecular weight
glucose polymer solution ( HMW ) compared to an isocaloric low molecular weight solution ( LMW ) or placebo ( PLA ) on subsequent cycling performance in female athletes . -
pubmed
The purpose of this paper is to review studies that examine conditions impacting the degree to which electronic
nicotine delivery system ( ENDS ) products substitute for conventional cigarettes in the Experimental Tobacco Marketplace (
ETM ) . -
pubmed
Overall , the
ETM provides a unique method to explore and identify the conditions by which various
nicotine products may interact with one another that mimics the real world . -
pubmed
In addition , the
ETM permits the efficacy of a broad range of potential
nicotine policies and regulations to be measured prior to governmental implementation . -
pubmed
Abiraterone could not bind and inhibit the
17 & amp;
alpha ;- hydroxylase activity of the
CYP17A1 - V366 M mutant . -
pubmed
The V366 M mutant also explained the effectiveness of the anti -
prostate cancer drug
abiraterone as a potent inhibitor of
CYP17A1 by binding tightly at the active site in the WT enzyme . -
pubmed
The major cellular
fatty acid was summed feature 8 (
C18 : 1omega7c and/or
C18 : 1omega6c ) , representing 70 % of the total
fatty acids . -
pubmed
We developed a structure - activity relationship (
SAR ) study of the
thiophene molecule 5A. -
pubmed
major promastigotes was associated with generation of reactive
oxygen species ( ROS ) , and in silico docking studies suggested that 5D may play a role in inhibiting
trypanothione reductase . -
pubmed
Copper levels affect targeting of
hypoxia - inducible factor 1alpha to the promoters of
hypoxia - regulated genes . -
pubmed
Previous studies have demonstrated that in
hypoxia ,
copper is required for the expression of
BNIP3 but not for that of
IGF2 Here , using ChIP assays , computational analyses ,
luciferase reporter assays , and real - time quantitative RT - PCR , we sought to better understand how
copper regulates the differential target gene selectivity of
HIF-1alpha . -
pubmed
The binding of
HIF-1alpha to
hypoxia - responsive element ( HRE ) sites in the
BNIP3 or
IGF2 gene promoter in high- or low -
copper conditions was examined . -
pubmed
Tetraethelenepentamine (
TEPA)-mediated reduction in
copper concentration did not affect
hypoxia- or
CoCl2 - induced
HIF-1alpha accumulation . -
pubmed
However , the
copper reduction did suppress the binding of
HIF-1alpha to the HRE ( -412/-404 ) in
BNIP3 but not the binding of
HIF-1alpha to the HRE ( -354/-347 ) in
IGF2 In summary , our findings uncovered the mechanistic basis for differential
HIF-1alpha - mediated regulation of
BNIP3 and
IGF2 , indicating that
copper regulates target gene selectivity of
HIF-1alpha at least in part by affecting
HIF-1alpha binding to its cognate HRE in the promoters of these two genes . -
pubmed
Hepatitis B serological test for
HBsAg ,
HBcAb ,
HBsAb ,
HBeAg and
HbeAb was undertaken using
Hepatitis B test kit and capillary blood from the participants . -
pubmed
The general prevalence of
HBcAb ,
HBsAg and
HBsAb was found to be 3.6 , 2.6 and 19.8 % respectively . -
pubmed
BMP2 - loaded
titania nanotubes coating with pH - responsive multilayers for
bacterial infections inhibition and osteogenic activity improvement . -
pubmed